title: Vaccination against the nematode Haemonchus contortus with a thiol-binding fraction from the excretory/secretory products (ES).
abstract: Fractionated excretory/secretory products (ES) of adult Haemonchus contortus were evaluated as protective antigens. The proteins were successively eluted from a Thiol Sepharose column using 25 mM cysteine followed by 25 mM Dl-dithiothreitol (DTT). Sheep were vaccinated three times and challenged with 5000 third stage infective larvae (L3) of H. contortus. Highest level of protection was found in sheep vaccinated with the DTT-eluted fraction in which egg output and worm burden were reduced by 52 and 50%, respectively, compared to the adjuvant control group. There was a positive correlation between fecundity (number of eggs per female) and the cumulative EPG or worm burden. Serum and mucus antibody levels of ES-specific immunoglobulins increased after immunizations and after challenge for IgG, IgA and IgE. The harvesting of H. contortus from animals clustered per group revealed the presence of cysteine protease activity in the ES of all groups but in addition to that, metalloprotease activity was also detected in the groups vaccinated with the DTT-eluted fraction, total ES and adjuvant only, in contrast to previous batches of ES (completely inhibited by E64) obtained from non vaccinated animals.
authors.affiliation: Division of Parasitology and Tropical Veterinary Medicine, Department of Infectious Diseases and Immunology, Utrecht University, PO Box 80165, 3508 TD Utrecht, The Netherlands.
authors.forename: N
authors.initials: N
authors.lastname: Bakker
authors.forename: L
authors.initials: L
authors.lastname: Vervelde
authors.forename: K
authors.initials: K
authors.lastname: Kanobana
authors.forename: D P
authors.initials: DP
authors.lastname: Knox
authors.forename: A W C A
authors.initials: AW
authors.lastname: Cornelissen
authors.forename: E
authors.initials: E
authors.lastname: de Vries
authors.forename: A P
authors.initials: AP
authors.lastname: Yatsuda
mesh_terms: D000018:Abomasum / Q000469:parasitology; D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000737:chemistry* / Q000276:immunology*; D002847:Chromatography, Agarose; D003546:Cysteine Endopeptidases / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D006188:Haemonchiasis / Q000276:immunology / Q000517:prevention & control* / Q000662:veterinary*; D006189:Haemonchus / Q000737:chemistry* / Q000276:immunology*; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D018655:Lymphocyte Count; D009093:Mucus / Q000276:immunology; D010270:Parasite Egg Count; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology* / Q000469:parasitology / Q000517:prevention & control*; D013438:Sulfhydryl Compounds / Q000378:metabolism
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D013438:Sulfhydryl Compounds; D003546:Cysteine Endopeptidases