title: Assessment of Chlamydia trachomatis prevalence by cell culture and transcription-mediated amplification in symptomatic women.
abstract: OBJECTIVE
  The frequency of Chlamydia trachomatis in women with mucopurulent discharge was determined by a cell culture technique and a transcription-mediated amplification (TMA) assay in endocervical swab specimens.
  
  
  METHODS
  Endocervical swab specimens were obtained from 116 symptomatic patients with genitourinary complaints or abdominal pain. All of the women were married, with an age range of between 19 and 44 (median 29) years. The cell culture assay was used in all specimens. For 75 specimens the TMA assay was also performed.
  
  
  RESULTS
  Positive cell culture test results were obtained in 6 (5.2%) patients. Among 75 specimens, 2 were positive by both TMA and culture assays, while 1 specimen was positive only by the culture assay. Of those positive for C. trachomatis, 5 were in the 19- to 25-year age group, and 1 was in the >25-year age group. All of the patients with positive results were of low socioeconomic status.
  
  
  CONCLUSIONS
  This study revealed a relatively low rate of C. trachomatis infections in symptomatic married women in Turkey. A commercial TMA assay failed to identify all positive patients, in contrast to a 'gold standard' culture assay used in patients having such infections.
authors.affiliation: Department of Microbiology and Clinical Microbiology, Ege University Medical Faculty, Izmir, Turkey. candanc2001@yahoo.com
authors.forename: Candan
authors.initials: C
authors.lastname: Cicek
authors.forename: Imre
authors.initials: I
authors.lastname: Altuglu
authors.forename: Tijen
authors.initials: T
authors.lastname: Ozacar
authors.forename: Kahraman
authors.initials: K
authors.lastname: Kolday
authors.forename: Namik
authors.initials: N
authors.lastname: Demir
authors.forename: Altinay
authors.initials: A
authors.lastname: Bilgic
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D006801:Humans; D014158:Transcription, Genetic; D014421:Turkey; D014626:Vaginal Smears
chemical_list: D004269:DNA, Bacterial