Anoxia induces matrix shrinkage accompanied by an increase in light scattering in isolated brain mitochondria.
It is important to monitor mitochondrial conditions, and light scattering (LS) measurements have been applied to the detection of morphological changes in mitochondria in vivo. Little is known about the morphological and LS responses of brain mitochondria to oxygen withdrawal, a critical factor in cell death. We have therefore investigated the morphological and LS responses of isolated brain mitochondria to anoxia. Anoxia induced an increase in LS, reflecting mitochondrial matrix shrinkage. This response was reversible, but was reduced by adding digitonin, which disrupted the outer membrane selectively. This suggested that integrity of the outer membrane was necessary for the matrix response. We further examined the effects of Mg2+ and ATP on the responses because both exist in cells and modulate the changes in matrix volume. Although Mg2+ and ATP reduced the rates of increase and decrease in LS, respectively, the magnitudes of the increases in LS caused by anoxia stayed at over 80% of the control level (no Mg2+) in the presence of Mg2+ and ATP. This suggested that the increase in LS occurred in cells containing Mg2+ and ATP during anoxia. In contrast, that caused by inhibitors of the electron transport chain was reduced to below 30% of the control level in the presence of Mg2+. The present in vitro study provides a basis for interpretation of LS signals from mitochondria in brain research during oxygen withdrawal.
Fujii
Fumihiko
F
Biophysics, Research Institute for Electronic Science, Hokkaido University, Sapporo 060-0812, Japan. ffujii@imd.es.hokudai.ac.jp
Nodasaka
Yoshinobu
Y
Nishimura
Goro
G
Tamura
Mamoru
M
D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D001923:Brain Chemistry; D003593:Cytoplasm / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D004072:Digitonin / Q000494:pharmacology; D004579:Electron Transport / Q000187:drug effects / Q000502:physiology; D004734:Energy Metabolism / Q000187:drug effects / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D002534:Hypoxia, Brain / Q000378:metabolism* / Q000473:pathology*; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D008029:Lighting; D008274:Magnesium / Q000378:metabolism / Q000494:pharmacology; D008297:Male; D008854:Microscopy, Electron; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology* / Q000648:ultrastructure; D010101:Oxygen Consumption / Q000187:drug effects; D010775:Photic Stimulation; D051381:Rats; D017208:Rats, Wistar; D013347:Subcellular Fractions / Q000737:chemistry / Q000378:metabolism
D004791:Enzyme Inhibitors; D000255:Adenosine Triphosphate; D008274:Magnesium; D004072:Digitonin