Imaging of adenovirus-mediated expression of human sodium iodide symporter gene by 99mTcO4 scintigraphy in mice.
We have evaluated the feasibility of human sodium iodide symporter (hNIS) as a reporter gene in vitro and in vivo. Recombinant adenovirus encoding hNIS (Rad-hNIS) was introduced to FRO cell for 48 hours. Western blotting and 99mTcO4 uptake study revealed functional hNIS expression in the cell. Rad-hNIS was injected to BALB/c mice via tail vein. 99mTcO4 gamma scintigraphy, biodistribution study, and RT-PCR analysis demonstrated a preferential hepatic uptake of 99mTcO4, which was observed for up to one week. Thus, hNIS can be utilized as an effective reporter gene for noninvasive/repeated imaging, in combination with 99mTcO4.
Lee
Won Woo
WW
Department of Microbiology, University of Ulsan College of Medicine, Seoul, South Korea.
Moon
Dae Hyuk
DH
Park
So Yeon
SY
Jin
Jungsun
J
Kim
Sung Jin
SJ
Lee
Heuiran
H
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D005786:Gene Expression Regulation / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D007668:Kidney / Q000000981:diagnostic imaging / Q000196:embryology / Q000378:metabolism*; D008297:Male; D008657:Metabolic Clearance Rate; D051379:Mice; D008807:Mice, Inbred BALB C; D009928:Organ Specificity; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D011994:Recombinant Proteins / Q000378:metabolism; D013670:Sodium Pertechnetate Tc 99m / Q000493:pharmacokinetics*; D027981:Symporters / Q000235:genetics / Q000378:metabolism*; D013961:Thyroid Gland / Q000000981:diagnostic imaging / Q000378:metabolism*; D014018:Tissue Distribution; D014170:Transformation, Genetic / Q000235:genetics; D000099209:Sodium-Iodide Symporters
D019275:Radiopharmaceuticals; D011994:Recombinant Proteins; D013670:Sodium Pertechnetate Tc 99m; D027981:Symporters; D000099209:Sodium-Iodide Symporters