Catalytic Reaction of Basidiomycete Lentinula edodes Cytochrome P450, Le.CYP1 Enzyme Produced in Yeast.
The basidiomycete Lentinula edodes (Le.) cytochrome P450, Le.CYP1 was functionally expressed in Saccharomyces cerevisiae. The microsomal fraction containing Le.CYP1 was prepared from the recombinant yeast and the Le.CYP1 was analyzed. The 7-ethoxycoumarin and benzo(a)pyrene were found to be the substrates of Le.CYP1 enzyme. Le.CYP1 converted 7-ethoxycoumarin to 7-hydroxycoumarin.
Akiyama
Ryoko
R
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Kajiwara
Susumu
S
Shishido
Kazuo
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D001564:Benzo(a)pyrene / Q000378:metabolism; D002384:Catalysis; D003374:Coumarins / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism*; D018076:DNA, Complementary; D008861:Microsomes / Q000378:metabolism; D008969:Molecular Sequence Data; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D020942:Shiitake Mushrooms / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity
D003374:Coumarins; D018076:DNA, Complementary; D011994:Recombinant Proteins; C017299:7-ethoxycoumarin; D001564:Benzo(a)pyrene; D003577:Cytochrome P-450 Enzyme System