title: ORESTES are enriched in rare exon usage variants affecting the encoded proteins.
abstract: A significant fraction of the variability found in the human transcriptome is due to alternative splicing, including alternative exon usage (AEU), intron retention and use of cryptic splice sites. We present a comparison of a large-scale analysis of AEU in the human transcriptome through genome mapping of Open Reading Frame ESTs (ORESTES) and conventional ESTs. It is shown here that ORESTES probe low abundant messages more efficiently. In addition, most of the variants detected by ORESTES affect the structure of the corresponding proteins.
authors.affiliation: Ludwig Institute for Cancer Research, Sao Paulo Branch, Rua Prof Antonio Prudente 109, 4(o) andar, 01509-010, Sao Paulo, Brazil.
authors.forename: Noboru Jo
authors.initials: NJ
authors.lastname: Sakabe
authors.forename: Jorge E S
authors.initials: JE
authors.lastname: de Souza
authors.forename: Pedro A F
authors.initials: PA
authors.lastname: Galante
authors.forename: Paulo S L
authors.initials: PS
authors.lastname: de Oliveira
authors.forename: Fábio
authors.initials: F
authors.lastname: Passetti
authors.forename: Helena
authors.initials: H
authors.lastname: Brentani
authors.forename: Elisson C
authors.initials: EC
authors.lastname: Osório
authors.forename: André C
authors.initials: AC
authors.lastname: Zaiats
authors.forename: Maarten R
authors.initials: MR
authors.lastname: Leerkes
authors.forename: João Paulo
authors.initials: JP
authors.lastname: Kitajima
authors.forename: Ricardo R
authors.initials: RR
authors.lastname: Brentani
authors.forename: Robert L
authors.initials: RL
authors.lastname: Strausberg
authors.forename: Andrew J G
authors.initials: AJ
authors.lastname: Simpson
authors.forename: Sandro José
authors.initials: SJ
authors.lastname: de Souza
mesh_terms: D017398:Alternative Splicing; D005091:Exons / Q000235:genetics*; D015894:Genome, Human; D006801:Humans; D016366:Open Reading Frames / Q000235:genetics*; D014158:Transcription, Genetic