Expression of platelet-derived growth factor-BB in liver tissues of patients with chronic hepatitis B.
OBJECTIVE
To study the relationship between expression of platelet-derived growth factor-BB (PDGF-BB) and fibrogenesis in chronic hepatitis B.


METHODS
Hepatic tissues from 43 patients with chronic hepatitis B were embedded in paraffin. The sections were stained with HE and picric acid-sirius red to determine inflammatory activity and fibrosis stages. PDGF-BB expression was detected by immunohistochemistry and assessed semiquantatively. Levels of serum hyaluronic acid (HA), pro-collagen III (PCIII), collagen IV (IV-C) and laminin (LN) were examined by radioimmunoassay (RIA).


RESULTS
The expression level of PDGF-BB was found to be positively correlated with inflammatory activity, fibrosis stage and grade of histological findings (tau=0.58, 0.55, 0.55, P<0.01). The positive correlation was also observed between tissue level of PDGF-BB expression and contents of HA, PCIII, IV-C and LN in the circulation (r=0.52, 0.32, 0.40, 0.33, P<0.05).


CONCLUSIONS
PDGF-BB may play some role in the development and progression of liver fibrosis.
Lou
Song-Mei
SM
Department of Gastroenterology, Hangzhou First People's Hospital, Hangzhou 310006, Zhejiang Province, China. lousm_72@hotmail.com
Li
You-Ming
YM
Wang
Kai-Ming
KM
Cai
Wei-Min
WM
Weng
Hong-Lei
HL
D000077214:Becaplermin; D001706:Biopsy; D019694:Hepatitis B, Chronic / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008103:Liver Cirrhosis / Q000097:blood; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D020574:Proto-Oncogene Proteins c-sis
D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin