title: Kinetic properties and ammonium-dependent regulation of cytosolic isoenzymes of glutamine synthetase in Arabidopsis.
abstract: Glutamine synthetase (GS; EC 6.3.1.2) is a key enzyme of nitrogen assimilation, catalyzing the synthesis of glutamine from ammonium and glutamate. In Arabidopsis, cytosolic GS (GS1) was accumulated in roots when plants were excessively supplied with ammonium; however, the GS activity was controlled at a constant level. The discrepancy between the protein content and enzyme activity of GS1 was attributable to the kinetic properties and expression of four distinct isoenzymes encoded by GLN1;1, GLN1;2, GLN1;3 and GLN1;4, genes that function complementary to each other in Arabidopsis roots. GLN1;2 was the only isoenzyme significantly up-regulated by ammonium, which correlated with the rapid increase in total GS1 protein. GLN1;2 was localized in the vasculature and exhibited low affinities to ammonium (Km = 2450 +/- 150 microm) and glutamate (Km = 3.8 +/- 0.2 mm). The expression of the counterpart vascular tissue-localizing low affinity isoenzyme, GLN1;3, was not stimulated by ammonium; however, the enzyme activity of GLN1;3 was significantly inhibited by a high concentration of glutamate. By contrast, the high affinity isoenzyme, GLN1;1 (Km for ammonium < 10 microm; Km for glutamate = 1.1 +/- 0.4 mm) was abundantly accumulated in the surface layers of roots during nitrogen limitation and was down-regulated by ammonium excess. GLN1;4 was another high affinity-type GS1 expressed in nitrogen-starved plants but was 10-fold less abundant than GLN1;1. These results suggested that dynamic regulations of high and low affinity GS1 isoenzymes at the levels of mRNA and enzyme activities are dependent on nitrogen availabilities and may contribute to the homeostatic control of glutamine synthesis in Arabidopsis roots.
authors.affiliation: RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
authors.forename: Keiki
authors.initials: K
authors.lastname: Ishiyama
authors.forename: Eri
authors.initials: E
authors.lastname: Inoue
authors.forename: Akiko
authors.initials: A
authors.lastname: Watanabe-Takahashi
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Obara
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Yamaya
authors.forename: Hideki
authors.initials: H
authors.lastname: Takahashi
mesh_terms: D017360:Arabidopsis / Q000201:enzymology*; D015536:Down-Regulation; D004789:Enzyme Activation; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D018517:Plant Roots / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D007527:Isoenzymes; D000644:Quaternary Ammonium Compounds; D005974:Glutamate-Ammonia Ligase