The primary structure of a novel goose-type lysozyme from rhea egg white.
G-type lysozyme is a hydrolytic enzyme sharing a similar tertiary structure with plant chitinase. To discover the relation of function and structure, we analyzed the primary structure of new G-type lysozyme. The complete 185 amino acid residues of lysozyme from rhea egg white were sequenced using the peptides hydrolyzed by trypsin, V8 protease, and cyanogen bromide. Rhea lysozyme had sequence similarity to ostrich, cassowary, goose, and black swan, with 93%, 90%, 83%, and 82%, respectively. The six substituted positions were newly found at positions 3 (Asn), 9 (Ser), 43 (Arg), 114 (Ile), 127 (Met), and 129 (Arg) when compared with ostrich, cassowary, goose, and black swan lysozymes. The amino acid substitutions of rhea lysozyme at subsite B were the same as ostrich and cassowary lysozymes (Ser122 and Met123). This study was also constructed in a phylogenetic tree of G-type lysozyme that can be classified into at least three groups of this enzyme, namely, group 1; rhea, ostrich, and cassowary, group 2; goose, black swan, and chicken, and group 3; Japanese flounder. The amino acid sequences in assembled three alpha-helices found in this enzyme group (Thammasirirak, S., Torikata, T., Takami, K., Murata, K., and Araki, T., Biosci. Biotechnol. Biochem., 66, 147-156 (2002)) were also highly conserved, so that they were considered to be important for the formation of the hydrophobic core structure of the catalytic site and for maintaining a similar three-dimensional structure in this enzyme group.
Pooart
Jureerut
J
Department of Biochemistry, School of Agriculture, Kyushu Tokai University, Kumamoto, Japan.
Torikata
Takao
T
Araki
Tomohiro
T
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001717:Birds; D002688:Chitinases / Q000737:chemistry; D004527:Egg Proteins / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009113:Muramidase / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry; D011487:Protein Conformation; D020311:Rheiformes; D017386:Sequence Homology, Amino Acid; D040681:Structural Homology, Protein
D000596:Amino Acids; D004527:Egg Proteins; D010940:Plant Proteins; D002688:Chitinases; D009113:Muramidase