title: Evolution of the gene encoding mitochondrial intermediate peptidase and its cosegregation with the A mating-type locus of mushroom fungi.
abstract: The high level of DNA polymorphism at the mating-type loci of mushroom fungi has made the cloning of mating-type genes difficult. As an alternative to strategies that employ sequence conservation, an approach utilizing conserved gene order could facilitate the cloning of A mating-type genes from mushroom fungi. It has been shown that a gene encoding a mitochondrial intermediate peptidase (MIP) is very close ( < 1 kbp) to the A mating-type locus of two model mushroom species. In this study, the cosegregation of MIP and the A mating-type locus was studied by genotyping progeny of seven additional mushroom species using PCR and genetic crosses. No evidence of any recombination between MIP and the A mating-type locus was detected among all seven species. Phylogenetic analysis of MIP sequences from diverse mushroom species agrees with the current organismal phylogeny, suggesting the sequences are generally orthologous.
authors.affiliation: Department of Biology, Box 90338, Duke University, Durham, NC 27708, USA. tyj2@duke.edu
authors.forename: Timothy Y
authors.initials: TY
authors.lastname: James
authors.forename: Ursula
authors.initials: U
authors.lastname: Kües
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Rehner
authors.forename: Rytas
authors.initials: R
authors.lastname: Vilgalys
mesh_terms: D000363:Agaricales / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D014644:Genetic Variation; D000072235:Mating Factor; D008666:Metalloendopeptidases / Q000235:genetics*; D010455:Peptides / Q000235:genetics*; D010802:Phylogeny
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; D010455:Peptides; D000072235:Mating Factor; D008666:Metalloendopeptidases; C068038:mitochondrial intermediate peptidase