E2 quasispecies specificity of hepatitis C virus association with allografts immediately after liver transplantation.
It is unknown whether all hepatitis C virus (HCV) quasispecies variants found within patient serum have equal capacity to associate with the liver after transplantation; however, in vitro models of HCV infection suggest that variations in the hypervariable region 1 (HVR1) of the second envelope protein (E2) may be important in infectivity. The hypothesis of the current study is that the two hypervariable regions (HVR1 and HVR2) within E2 are important in the initial virus-liver interaction, and, therefore, certain HCV quasispecies variants will be isolated from the liver after reperfusion. In 8 patients with end-stage liver disease secondary to HCV infection, HCV envelope quasispecies were determined from intraoperative serum samples obtained before the anhepatic phase of transplantation and from liver biopsies 1.5 to 2.5 hours after the transplanted liver was perfused. Explanted (native) liver biopsies were taken as a control. Sequence analysis was performed on clones of specific HCV reverse transcriptase-polymerase chain reaction products spanning HVR1 and HVR2 of the E2 protein. HVR1 was more variable than HVR2 for all samples. Quasispecies isolated from postperfusion liver differed more from serum than did explanted liver quasispecies at HVR1 (P = 0.03) but not at HVR2 (P = 0.2). Comparison of HVR1 sequences from postperfusion liver versus serum revealed significantly less HVR1 genetic complexity and diversity (P = 0.02 and P = 0.04, respectively). Immediately after transplantation but before actual infection, liver allografts select out from the infecting serum inoculum a less heterogeneous, more closely related population of quasispecies variants.
Hughes
Michael G
MG
University of Virginia, Department of Surgery, Surgical Infectious Disease Laboratory, Charlottesville, VA, USA. mgh6a@virginia.edu
Rudy
Christine K
CK
Chong
Tae W
TW
Smith
Robert L
RL
Evans
Heather L
HL
Iezzoni
Julia C
JC
Sawyer
Robert G
RG
Pruett
Timothy L
TL
D000595:Amino Acid Sequence; D001773:Blood Cells / Q000378:metabolism; D016384:Consensus Sequence; D014644:Genetic Variation; D016174:Hepacivirus / Q000378:metabolism*; D006526:Hepatitis C / Q000150:complications; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000821:virology*; D017093:Liver Failure / Q000821:virology; D016031:Liver Transplantation; D008969:Molecular Sequence Data; D011184:Postoperative Period; D013997:Time Factors; D014184:Transplantation, Homologous; D014759:Viral Envelope Proteins / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000097:blood / Q000235:genetics / Q000378:metabolism
C400562:HVR1 protein, Hepatitis C virus; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C091015:glycoprotein E2, Hepatitis C virus