title: [A study of injectable autogenous tissue-engineered bone].
abstract: OBJECTIVE
  To investigate the utilization of carrier for delivering osteoblasts and creating autogenous bone tissue in ectopic site of animal via injection.
  
  
  METHODS
  Bone marrow cells harvested from iliac bone of New Zealand rabbits were induced to differentiate into marrow stromal osteoblasts. The osteoblasts were mixed with 1.5% alginate sodium solution to generate osteoblasts/alginate composites with final cellular density of 4 x 10(9)/L. Calcium chloride was used as cross-linking agent to gel aqueous alginate solution. The marrow stromal osteoblasts/alginate composites were injected into the dorsal subcutaneous tissue of rabbits with autogenous cells transplantation. The samples were examined with X-ray and histological analysis.
  
  
  RESULTS
  Four, eight and twelve weeks after injection, the hard knobbles were easily palpated under the dorsal skin of animals. On X-ray photograph the samples showed calcified image with more density than surrounding soft tissue, new bone formation was observed in the osteoblasts/alginate composites in histological analysis. The osteogenesis was in association with regenerated hematopoietic bone marrow.
  
  
  CONCLUSIONS
  These results demonstrate that new bone tissue could be created through the injection of alginate sodium treated with autogenous marrow stromal osteoblasts.
authors.affiliation: Department of Oral and Maxillofacial Surgery, College of Stomatology, The Fourth Military Medical University, Xi'an, China.
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Yang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Cao
authors.forename: Qi-chun
authors.initials: QC
authors.lastname: Qian
authors.forename: Li-hui
authors.initials: LH
authors.lastname: Tang
authors.forename: Lian-jia
authors.initials: LJ
authors.lastname: Yang
authors.forename: Tian-qiu
authors.initials: TQ
authors.lastname: Mao
mesh_terms: D000464:Alginates / Q000008:administration & dosage*; D000818:Animals; D020723:Glucuronic Acid / Q000008:administration & dosage*; D006603:Hexuronic Acids / Q000008:administration & dosage*; D010006:Osteoblasts / Q000637:transplantation*; D010012:Osteogenesis; D011817:Rabbits; D023822:Tissue Engineering
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid