Improved survival of orthotopic liver allograft in swine by addition of trophic factors to University of Wisconsin solution.
Serum-free preservation media such as University of Wisconsin (UW) may cause tissue damage through trophic factor (TF) deprivation. This study evaluated whether the addition of TFs to UW solution improves liver graft quality after extended cold preservation time in pigs. UW solution was supplemented with epidermal growth factor, insulin-like growth factor-1, nerve growth factor-beta, bactenecin, and substance P to create TF-supplemented (TFS) UW. Orthotopic liver transplantation was performed after 18 hr of static cold storage at 4 degrees C in UW (n=7) or TFS-UW (n=7) solution. Recipients of grafts preserved with TFS-UW demonstrated significantly better 5-day survival (57%) than those preserved with UW alone (14%) (P<0.05). Adenosine triphosphate content in grafts preserved in TFS-UW was significantly higher than in grafts preserved in UW (17.4+/-5.0 vs. 4.8+/-1.2 nmol/mg protein, respectively) (P<0.05). This study showed that the addition of TFs to UW solution allowed a significant extension of cold ischemic time in pigs.
Ambiru
Satoshi
S
Laboratory of Experimental Surgery, Faculté de Médecine, Université Catholique de Louvain, Brussels, Belgium.
Uryuhara
Kenji
K
Talpe
Stéphanie
S
Dehoux
Jean-Paul
JP
Jacobbi
Louise
L
Murphy
Christopher J
CJ
McAnulty
Jonathan F
JF
Gianello
Pierre
P
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005978:Glutathione; D006085:Graft Survival / Q000187:drug effects / Q000502:physiology*; D006133:Growth Substances / Q000494:pharmacology*; D007328:Insulin; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D008099:Liver / Q000187:drug effects; D016031:Liver Transplantation / Q000502:physiology*; D023421:Models, Animal; D020932:Nerve Growth Factor / Q000494:pharmacology; D019188:Organ Preservation Solutions; D010456:Peptides, Cyclic / Q000494:pharmacology; D011887:Raffinose; D013373:Substance P / Q000494:pharmacology; D013552:Swine
D006133:Growth Substances; D007328:Insulin; D019188:Organ Preservation Solutions; D010456:Peptides, Cyclic; C055206:University of Wisconsin-lactobionate solution; C056454:bactenecin; D013373:Substance P; D004815:Epidermal Growth Factor; D000493:Allopurinol; D007334:Insulin-Like Growth Factor I; D020932:Nerve Growth Factor; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose