The NIA cDNA project in mouse stem cells and early embryos.
A catalog of mouse genes expressed in early embryos, embryonic and adult stem cells was assembled, including 250000 ESTs, representing approximately 39000 unique transcripts. The cDNA libraries, enriched in full-length clones, were condensed into the NIA 15 and 7.4K clone sets, freely distributed to the research community, providing a standard platform for expression studies using microarrays. They are essential tools for studying mammalian development and stem cell biology, and to provide hints about the differential nature of embryonic and adult stem cells.
Carter
Mark G
MG
Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
Piao
Yulan
Y
Dudekula
Dawood B
DB
Qian
Yong
Y
VanBuren
Vincent
V
Sharov
Alexei A
AA
Tanaka
Tetsuya S
TS
Martin
Patrick R
PR
Bassey
Uwem C
UC
Stagg
Carole A
CA
Aiba
Kazuhiro
K
Hamatani
Toshio
T
Matoba
Ryo
R
Kargul
George J
GJ
Ko
Minoru S H
MS
D000818:Animals; D003001:Cloning, Molecular; D004622:Embryo, Mammalian; D015723:Gene Library; D023281:Genomics; D006801:Humans; D051379:Mice / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D013234:Stem Cells