title: Concatenation cDNA sequencing for transcriptome analysis.
abstract: We describe a high-throughput cDNA sequencing pipeline (http://www.hgsc.bcm.tmc.edu/projects/cdna) built in response to the emerging need for rapid sequencing of large cDNA collections. Using this strategy cDNA inserts are purified and joined through concatenation into large molecules. These 'pseudo-BACs' are subjected to random shotgun sequencing whereby the majority of cDNA inserts in the pool are sequenced. Using this concatenation cDNA sequencing platform, we have contributed more than 13000 full-length cDNA sequences from human and mouse to the Mammalian Gene Collection (MGC).
authors.affiliation: Human Genome Sequencing Center, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA. preethig@bcm.tmc.edu
authors.forename: Preethi H
authors.initials: PH
authors.lastname: Gunaratne
authors.forename: Jia Qian
authors.initials: JQ
authors.lastname: Wu
authors.forename: Angela M
authors.initials: AM
authors.lastname: Garcia
authors.forename: Steven
authors.initials: S
authors.lastname: Hulyk
authors.forename: Kim C
authors.initials: KC
authors.lastname: Worley
authors.forename: Judith F
authors.initials: JF
authors.lastname: Margolin
authors.forename: Richard A
authors.initials: RA
authors.lastname: Gibbs
mesh_terms: D003001:Cloning, Molecular; D018076:DNA, Complementary; D044708:DNA, Concatenated; D005821:Genetic Techniques; D006801:Humans; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D044708:DNA, Concatenated