Special features of the DNA-hydrolyzing activity of the antibodies in systemic lupus erythematosus.
Two types of IgG anti-DNA antibodies exhibiting DNA-hydrolyzing activity have been isolated from blood serum of patients with systemic lupus erythematosus. This DNase activity of antibodies differs from serum DNases by the non-processive mode, temperature resistance, pH optimum, and the rate of DNA hydrolysis. It is suggested that the anti-DNA antibody molecule possessing DNase activity contains two sites: one site determines specificity of antibody-DNA interaction, whereas the other is responsible for manifestation of the catalytic activity.
Nevzorova
T A
TA
Kazan State University, Kazan 420008, Russia. Tatyana.Nevzorova@ksu.ru
Temnikov
D A
DA
Vinter
V G
VG
D000974:Antibodies, Antinuclear / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007074:Immunoglobulin G / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008180:Lupus Erythematosus, Systemic / Q000276:immunology* / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013696:Temperature
D000974:Antibodies, Antinuclear; D007074:Immunoglobulin G; D004247:DNA; D003851:Deoxyribonucleases