Homologous and heterologous antibody responses to lipopolysaccharide after enterohemorrhagic Escherichia coli infection.
To evaluate antibody responses against lipopolysaccharide (LPS: O157, O26, and O111) in enterohemorrhagic Escherichia coli(EHEC) infection, sera of 24 schoolchildren associated with the Morioka outbreak in 1997 and of 74 sporadic patients suspected of having EHEC infection were examined. Using a positive standard serum, quantitative evaluation of LPS antibodies by an enzyme-linked immunosorbent assay (ELISA) was established. High levels of specific IgM and IgA antibodies against homologous E. coli LPS were present in the acute period and are characteristic of EHEC. This could be used for the serological diagnosis of EHEC infection, except for early infants and the elderly. In addition to the specific homologous response, multiple antibody responses against different serotypes other than those isolated were demonstrated in many cases by qualitative analysis using Western blotting.
Tsutsumi
Reiko
R
Department of Microbiology, School of Medicine, Iwate Medical University, USA. ssato@iwate-med.ac.jp
Ichinohe
Naoko
N
Shimooki
Osamu
O
Obata
Fumiko
F
Takahashi
Kiomi
K
Inada
Katsuya
K
Sasaki
Mika
M
Sato
Shigehiro
S
Chida
Shoichi
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood*; D015153:Blotting, Western; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000276:immunology*; D019453:Escherichia coli O157 / Q000276:immunology* / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007223:Infant; D007700:Kinetics; D008070:Lipopolysaccharides / Q000276:immunology*; D008875:Middle Aged; D019081:O Antigens / Q000276:immunology*
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D019081:O Antigens