title: Tailoring biomaterial compatibility: in vivo tissue response versus in vitro cell behavior.
abstract: Biocompatibility relies essentially on surface phenomena, represented by cell-cell, cell-material and material (polymer)-protein interactions. An in vivo and in vitro experimental investigation was carried out on the biomaterials of two different classes with a good potential for in situ utilisation. Non-resorbable (Polypyrrole, Polyaniline, Polyimide) and resorbable (PLLA-PDXO-PLLA) materials for tissue engineering were studied for their overall tissue tolerance and cellular interactions. These non-resorbable polymers conceived for biosensor applications and implantable drug-delivery systems are intrinsically conductive. The PLLA-PDXO-PLLA triblock copolymer showed interesting tensile properties for bone and cartilage tissue engineering due to the presence of 1,5-dioxepan-2-one. In vitro and in vivo parallel studies showed an interesting correspondence: a) the cells in contact with the resorbable material that appeared to be capable of migratory-regenerative aspects in vitro exhibited good compatibility in vivo; whereas b) the non-resorbable materials, which are designed to remain in situ in vivo, were seen to have the potential to represent an adverse factor (inflammation, fibrotic reactions) that correlated with some aspects of cell behaviour in vitro.
authors.affiliation: Institute of Normal Human Morphology, Polytechnic University of Marche, Ancona, Italy. belmonte@med.univpm.it
authors.forename: M
authors.initials: M
authors.lastname: Mattioli-Belmonte
authors.forename: G
authors.initials: G
authors.lastname: Giavaresi
authors.forename: G
authors.initials: G
authors.lastname: Biagini
authors.forename: L
authors.initials: L
authors.lastname: Virgili
authors.forename: M
authors.initials: M
authors.lastname: Giacomini
authors.forename: M
authors.initials: M
authors.lastname: Fini
authors.forename: F
authors.initials: F
authors.lastname: Giantomassi
authors.forename: D
authors.initials: D
authors.lastname: Natali
authors.forename: P
authors.initials: P
authors.lastname: Torricelli
authors.forename: R
authors.initials: R
authors.lastname: Giardino
mesh_terms: D020341:Absorbable Implants; D000814:Aniline Compounds / Q000494:pharmacology; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D018929:Cell Culture Techniques; D002460:Cell Line; D005260:Female; D006571:Heterocyclic Compounds / Q000494:pharmacology; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D008422:Materials Testing; D011091:Polyesters / Q000494:pharmacology; D011108:Polymers / Q000494:pharmacology; D011758:Pyrroles / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012117:Resins, Synthetic / Q000494:pharmacology; D013718:Tensile Strength
chemical_list: D000814:Aniline Compounds; D001672:Biocompatible Materials; D006571:Heterocyclic Compounds; D011091:Polyesters; D011108:Polymers; D011758:Pyrroles; D012117:Resins, Synthetic; C416807:polyaniline; C067635:polypyrrole; C091881:1,5-dioxepan-2-one; C029945:polyimide resin; C033616:poly(lactide)