Detection of genetic variation in Taenia solium.
Genetic variability among Taenia solium isolates was studied in 160 cysticerci from 6 pigs, 4 from Mexico, 1 from Honduras, and 1 from Tanzania. Random amplified polymorphic DNA (RAPD) analysis performed with 4 commercial primers showed 88% polymorphic loci and an average heterozygosity of 0.077; however, several alleles were fixed within each isolate. Linkage disequilibrium analysis indicated that 3 of the 6 isolates had a random association of alleles, whereas the other 3 had a clonal structure. These results suggest the existence of local lineages in T. solium, with events of genetic recombination within them.
Maravilla
Pablo
P
Direccion de Investigacion, Hospital General Dr. Manuel Gea Gonzalez, SSA, 11400 Mexico, DF, Mexico.
Souza
Valeria
V
Valera
Aldo
A
Romero-Valdovinos
Mirza
M
Lopez-Vidal
Yolanda
Y
Dominguez-Alpizar
Jose Luis
JL
Ambrosio
Javier
J
Kawa
Simon
S
Flisser
Ana
A
D000818:Animals; D016000:Cluster Analysis; D003551:Cysticercosis / Q000469:parasitology / Q000662:veterinary; D003552:Cysticercus / Q000235:genetics; D018509:DNA, Helminth / Q000032:analysis; D014644:Genetic Variation; D006721:Honduras; D015810:Linkage Disequilibrium; D008800:Mexico; D009010:Monte Carlo Method; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology; D041201:Taenia solium / Q000145:classification / Q000235:genetics*; D013636:Tanzania
D018509:DNA, Helminth