A technical trick for studying proteomics in parallel to transcriptomics in symbiotic root-fungus interactions.
We have developed a protocol in which proteins and mRNA can be analyzed from single root samples. This experimental design was validated in arbuscular mycorrhiza by comparing the proteins profiles obtained with those from a classical protein extraction process. It is a step forward to make simultaneous proteome and transcriptiome profiling possible.
Dumas-Gaudot
Eliane
E
UMR 1088 INRA/CNRS 5184/U, Bourgogne (Plante-Microbe- Environnement) INRA-CMSE, Dijon, France. dumas@epoisses.inra.fr
Amiour
Nardjis
N
Weidmann
Stéphanie
S
Bestel-Corre
Gwénaëlle
G
Valot
Benoit
B
Lenogue
Sébastien
S
Gianinazzi-Pearson
Vivienne
V
Gianinazzi
Silvio
S
D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000032:analysis; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D029902:Medicago / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D038821:Mycorrhizae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis*; D013559:Symbiosis / Q000502:physiology
D005656:Fungal Proteins; D020543:Proteome; D012333:RNA, Messenger