title: Production of a calibrant certified reference material for determination of the estrogenic mycotoxin zearalenone.
abstract: Several previous interlaboratory studies in the field of mycotoxin analysis have revealed considerable problems, apparent as high between-laboratory standard deviations, or rather non-comparable and non-traceable results. A major reason is lack of proper calibrants for external calibration. Public awareness of substances that mimic or interfere with the activity of natural hormones (endocrine disrupters) has led to increased interest in mycotoxins with estrogenic potential, e.g. zearalenone (ZON). During a large-scale standard measurement and testing (SMT) project of the European Commission (EC) dealing with the preparation and certification of reference materials for determination of the mycotoxin ZON in maize, a ZON calibrant in acetonitrile was prepared and intensively checked for purity, homogeneity, and stability. Preparation of the material, study of its homogeneity and stability, and characterisation of the calibrant on the basis of its preparation, with discussion of the results obtained, are described in this paper. The certified value of 9.95 micro g mL(-1) for ZON in acetonitrile and its corresponding expanded uncertainty of +/-0.30 micro g mL(-1) were calculated in compliance with the Guide to the Expression of Uncertainty in Measurement (GUM).
authors.affiliation: Center for Analytical Chemistry, Institute for Agrobiotechnology (IFA-Tulln), Konrad Lorenz Strasse 20, 3430, Tulln, Austria.
authors.forename: Ralf Dieter
authors.initials: RD
authors.lastname: Josephs
authors.forename: Rudolf
authors.initials: R
authors.lastname: Krska
authors.forename: Susan
authors.initials: S
authors.lastname: MacDonald
authors.forename: Pete
authors.initials: P
authors.lastname: Wilson
authors.forename: Hans
authors.initials: H
authors.lastname: Pettersson
mesh_terms: D000704:Analysis of Variance; D002138:Calibration; D002568:Certification; D004355:Drug Stability; D004967:Estrogens / Q000737:chemistry*; D016014:Linear Models; D009183:Mycotoxins / Q000032:analysis* / Q000737:chemistry; D011786:Quality Control; D012015:Reference Standards; D003313:Zea mays / Q000737:chemistry; D015025:Zearalenone / Q000032:analysis* / Q000737:chemistry
chemical_list: D004967:Estrogens; D009183:Mycotoxins; D015025:Zearalenone