Analysis of benzene ethylamine derivatives in urine using the programmable dynamic liquid-phase microextraction (LPME) device.
An automated LPME device for a dynamic LPME method was manufactured and its extraction efficiency was tested using spiked urine samples. The developed home-made LPME device was a programmable automated syringe dispenser to overcome deteriorating precision and difficulties in manually manipulating the plunger repeatedly. To establish the optimum parameters for benzene ethylamines, the effects of sampling volume, solvent volume, pH, salt-effect, choice of solvents, plunger speed, and number of samplings were investigated. Good repeatabilities for the extraction of mephentermine, ephedrine, methoxyphenamine, selegiline, and bupropion were obtained and the RSD values were 2.4, 1.9, 1.3, 1.6 and 1.5% at a concentration of 3 microg mL(-1) in spiked urine samples, respectively. The limit of detection was below 0.05 microg mL(-1) for the investigated drugs. This developed device for LPME analysis gave good validation results and improved convenience.
Myung
Seung-Woon
SW
Doping Control Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul 136-791, Korea. swmyung@kist.re.kr
Yoon
Sung-Ho
SH
Kim
Myungsoo
M
D001555:Benzene Derivatives / Q000652:urine*; D016642:Bupropion / Q000652:urine; D001330:Electronic Data Processing; D004809:Ephedrine / Q000652:urine; D005021:Ethylamines / Q000652:urine*; D006801:Humans; D008616:Mephentermine / Q000652:urine; D008694:Methamphetamine / Q000031:analogs & derivatives* / Q000652:urine; D008832:Microchemistry / Q000379:methods; D015203:Reproducibility of Results; D012642:Selegiline / Q000652:urine; D012680:Sensitivity and Specificity; D015813:Substance Abuse Detection
D001555:Benzene Derivatives; D005021:Ethylamines; D016642:Bupropion; D012642:Selegiline; D008694:Methamphetamine; D004809:Ephedrine; C005157:methoxyphenamine; D008616:Mephentermine