title: Determination of butein in rat serum by high performance liquid chromatography.
abstract: A simple high performance liquid chromatography (HPLC) method was developed for the determination of butein in rat serum. The method involved the deproteinization followed by injection into a Luna C8 column. Butein was eluted at 3.8 min at a flow rate of 0.2 ml/min with the mobile phase of acetonitrile-ammonium formate (10mM, pH 3.0) (35:65, v/v). The standard curve was linear (r(2)=0.995) over the concentration range of 0.1-10 microg/ml. The coefficient of variation (CV) of intra- and inter-assay ranged from 2.7 to 7.5% and 6.0 to 7.5%, respectively. The limit of quantification was 0.1 microg/ml using a serum sample of 50 microl. This method was applied to a pharmacokinetic study after intravenous injection of butein (5mg/kg) to rats.
authors.affiliation: College of Pharmacy and Medicinal Resources Research Center, Wonkwang University, Shinyongdong, Iksan 570-749, South Korea.
authors.forename: Hye Won
authors.initials: HW
authors.lastname: Lee
authors.forename: Hye Young
authors.initials: HY
authors.lastname: Ji
authors.forename: Hong Il
authors.initials: HI
authors.lastname: Lee
authors.forename: Hae-Kyoung
authors.initials: HK
authors.lastname: Kim
authors.forename: Dong Hwan
authors.initials: DH
authors.lastname: Sohn
authors.forename: Youn-Chul
authors.initials: YC
authors.lastname: Kim
authors.forename: Hun Taeg
authors.initials: HT
authors.lastname: Chung
authors.forename: Hye Suk
authors.initials: HS
authors.lastname: Lee
mesh_terms: D000818:Animals; D002599:Chalcone / Q000031:analogs & derivatives* / Q000097:blood*; D047188:Chalcones; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
chemical_list: D047188:Chalcones; C040918:butein; D002599:Chalcone