title: Two digestive trypsins occur in three species of neotropical anophelines.
abstract: Trypsin activity increases in the midgut of Anopheles aquasalis, Anopheles albitarsis, and Anopheles darlingi after a bloodmeal. The activity returns to basal levels at the time the blood is completely digested. Affinity chromatography, reversed-phase high performance liquid chromatography (HPLC), and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were used to sequentially purify the mosquito trypsins found in the midguts at 24 h after feeding. Aminoterminal sequencing of the purified trypsins showed the occurrence of two distinct trypsins in the midgut of each of the mosquitoes studied. The sequences obtained are similar to those of the trypsins of other hematophagous insects.
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, CEP 05,508-900, Brazil.
authors.forename: A S
authors.initials: AS
authors.lastname: Caroci
authors.forename: E
authors.initials: E
authors.lastname: Calvo
authors.forename: P E
authors.initials: PE
authors.lastname: Ribolla
authors.forename: A G
authors.initials: AG
authors.lastname: De Bianchi
authors.forename: O
authors.initials: O
authors.lastname: Marinotti
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000145:classification / Q000201:enzymology* / Q000502:physiology; D001769:Blood / Q000469:parasitology*; D001938:Brazil; D017124:Conserved Sequence; D004063:Digestion / Q000502:physiology*; D005247:Feeding Behavior; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014357:Trypsin / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D014357:Trypsin