Characterization of Borrelia burgdorferi sensu lato from Novosibirsk region (West Siberia, Russia) based on direct PCR.
Borrelia burgdorferi sensu lato infecting Ixodes persulcatus ticks near Novosibirsk, Russia were detected using PCR with primers specific to 5S and 23S rRNA genes. Two genospecies, B. afzelii and B. garinii, were identified by the PCR-based restriction fragment length polymorphism analysis with Tru9-I restriction endonuclease. Comparison of the corresponding nucleotide sequences revealed considerable diversity of the 5S-23S intergenic spacer structure among B. garinii.
Livanova
Nnatalya Nikolaevna
NN
Institute of Systematics and Ecology of Animals, Novosibirsk, Russia. dob@eco.nsc.ru
Morozova
O'lga Vladimirovich
OV
Morozov
Igor' Vladimirovich
IV
Beklemishev
Anatoly Bonsovich
AB
Cabello
Felipe C
FC
Dobrotvorsky
Andrey Kirovich
AK
D000818:Animals; D001483:Base Sequence; D015748:Borrelia burgdorferi Group / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers; D018884:Ixodes / Q000382:microbiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012341:RNA, Ribosomal, 5S; D012689:Sequence Homology, Nucleic Acid; D012800:Siberia
D017931:DNA Primers; D012338:RNA, Ribosomal, 23S; D012341:RNA, Ribosomal, 5S