Dynamic ultrasound-assisted leaching of essential macro and micronutrient metal elements from animal feeds prior to flame atomic absorption spectrometry.
A method for routine analysis of essential metal elements in animal feeds using a dynamic and automated ultrasound-assisted extractor is proposed here. Owing to the different concentration of the essential metal elements in the samples, two macronutrients-namely, Ca and Mg-and three micronutrients or trace elements-namely, Fe, Cu and Zn-were selected as models for the optimisation and characterisation of the proposed method by using experimental design methodology. Moreover, the extraction kinetics and influence of the particle size on the extraction efficiency were studied. The detection and quantification limits ranged between 2.9 and 40 microg kg(-1) and between 7.9 and 95.5 microg kg(-1), respectively. The precision, expressed as repeatability relative standard deviation (RSD) and as within-laboratory RSD, ranged between 1.86 and 5.66% and between 5.06 and 6.15%, respectively. The proposed approach allows the extraction of these metal elements from animal feeds with extraction efficiencies similar to those provided by the AOAC Official Method 968.08, but with a drastic reduction in both the extraction time (18 min versus 4.5 h) and sample handling, and using smaller volumes of extractant (an acid aqueous solution).
Priego-Capote
F
F
Analytical Chemistry Division, University of Córdoba, Annex C-3 Building, Campus of Rabanales, 14071, Córdoba, Spain.
Luque de Castro
M D
MD
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D007700:Kinetics; D008670:Metals / Q000032:analysis*; D018977:Micronutrients / Q000032:analysis*; D010316:Particle Size; D015203:Reproducibility of Results; D013010:Sonication / Q000295:instrumentation; D013054:Spectrophotometry, Atomic / Q000379:methods*
D008670:Metals; D018977:Micronutrients