title: Integrated high capacity solid phase extraction-MS/MS system for pharmaceutical profiling in drug discovery.
abstract: A method is described for use in analysis of samples from pharmaceutical profiling of early drug discovery compounds. The method consists of a high capacity autosampler which injects samples into one of two solid phase extraction columns operated in parallel for alternating trapping, washing and elution into a tandem quadrupole mass spectrometer operated in multiple reaction monitoring (MRM) MS/MS mode. A primary method, which is useful for 80-90% of compounds, and a secondary method, which is useful for a majority of the remaining compounds, are described. No analytical HPLC column is used and the analysis rate is approximately 50 samples/h. Specificity is obtained using MRM analysis. Application of the method for high capacity analysis of metabolic stability samples is described.
authors.affiliation: Wyeth Research, CN8000, Princeton, NJ 08543-8000, USA. kernse@wyeth.com
authors.forename: Edward H
authors.initials: EH
authors.lastname: Kerns
authors.forename: Teresa
authors.initials: T
authors.lastname: Kleintop
authors.forename: David
authors.initials: D
authors.lastname: Little
authors.forename: Thomas
authors.initials: T
authors.lastname: Tobien
authors.forename: Larry
authors.initials: L
authors.lastname: Mallis
authors.forename: Li
authors.initials: L
authors.lastname: Di
authors.forename: Mei
authors.initials: M
authors.lastname: Hu
authors.forename: Yan
authors.initials: Y
authors.lastname: Hong
authors.forename: Oliver J
authors.initials: OJ
authors.lastname: McConnell
mesh_terms: D015195:Drug Design; D004355:Drug Stability; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000295:instrumentation / Q000379:methods*
chemical_list: D004364:Pharmaceutical Preparations