title: Specific detection of the toxigenic species Fusarium proliferatum and F. oxysporum from asparagus plants using primers based on calmodulin gene sequences.
abstract: Fusarium proliferatum and Fusarium oxysporum are the causal agents of a destructive disease of asparagus called Fusarium crown and root rot. F. proliferatum from asparagus produces fumonisin B1 and B2, which have been detected as natural contaminants in infected asparagus plants. Polymerase chain reaction (PCR) assays were developed for the rapid identification of F. proliferatum and F. oxysporum in asparagus plants. The primer pairs are based on calmodulin gene sequences. The PCR products from F. proliferatum and F. oxysporum were 526 and 534 bp long, respectively. The assays were successfully applied to identify both species from the vegetative part of the plants.
authors.affiliation: Institute of Sciences of Food Production, CNR, Viale Einaudi, 51, 70125 Bari, Italy. giuseppina.mule@ispa.cnr.it
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Mulè
authors.forename: Antonia
authors.initials: A
authors.lastname: Susca
authors.forename: Gaetano
authors.initials: G
authors.lastname: Stea
authors.forename: Antonio
authors.initials: A
authors.lastname: Moretti
mesh_terms: D027761:Asparagus Plant / Q000382:microbiology*; D001483:Base Sequence; D002147:Calmodulin / Q000235:genetics*; D017931:DNA Primers; D005670:Fusarium / Q000302:isolation & purification* / Q000472:pathogenicity*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity
chemical_list: D002147:Calmodulin; D017931:DNA Primers