sigma marY1, the LTR of the gypsy-type retroelement marY1 from the basidiomycete tricholoma matsutake, allows multicopy DNA integration in Lentinula edodes.
sigma marY1 is the LTR of the retroelement marY1 from the homobasidiomycete Tricholoma matsutake. Upon integration through transformation, pLC1-hph carrying a sigma marY1 derivative, sigma* marY1, conferred the hygromycin-resistant phenotype stronger than the vector without sigma* marY1 on Lentinula edodes. Based on the densitometric analysis after Southern hybridization, a copy number of the former construct integrated in the genome is much higher than that of the latter. We conclude that sigma marY1 allows multicopy DNA integration and will be useful in the genetic research on this fungal group.
Murata
Hitoshi
H
Department of Applied Microbiology and Mushroom Sciences, Forestry & Forest Products Research Institute, Ibaraki, Japan. murmur@ffpri.affrc.go.jp
Miyazaki
Yasumasa
Y
D000363:Agaricales / Q000235:genetics*; D004271:DNA, Fungal / Q000378:metabolism; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D018626:Retroelements; D020942:Shiitake Mushrooms / Q000235:genetics*; D020079:Terminal Repeat Sequences; D014170:Transformation, Genetic
D004271:DNA, Fungal; D018626:Retroelements