title: Temperature-dependent Raman spectra of collagen and DNA.
abstract: Raman spectra of collagen and DNA were discussed at different temperatures. The temperature-dependence of Raman intensity was obtained in the region from -150 to 200 degrees C. Four denaturation points at 0, 40, 68 and 90 degrees C of collagen and two peaks at 38 and 82 degrees C for DNA were obtained. The wavenumbers of many vibrational modes were found to increase for lower temperature, but the peak at 1302 cm(-1) of collagen and the peak at 1101 cm(-1) of DNA showed the opposite trend. In all of the vibrational modes of DNA, the bases showed the most sensitive to different temperatures and there is a pronounced shift of bands at 70 degrees C, the starting point of denaturation.
authors.affiliation: School of Physical Electronics, University of Electronic Science and Technology of China, Chengdu, Sichuan 610054, China. ruixindong@163.com
authors.forename: Ruixin
authors.initials: R
authors.lastname: Dong
authors.forename: Xunling
authors.initials: X
authors.lastname: Yan
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Pang
authors.forename: Shenggang
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D002417:Cattle; D003094:Collagen / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D013059:Spectrum Analysis, Raman; D013696:Temperature; D013950:Thymus Gland / Q000737:chemistry
chemical_list: D003094:Collagen; D004247:DNA