title: Determination of oxidative protein and lipid damage in adult hypopituitary patients with GH deficiency.
abstract: The aim of this study is to determine oxidative protein and lipid damage in adult hypopituitary GH-deficient patients. Eighteen hypopituitary GH-deficient--otherwise healthy-adults on conventional replacement therapy other than GH (9 male, 9 female, age 41.8 +/- 16.4 yr) and 18 healthy subjects (6 male, 12 female, age 40.3 +/- 16.2 yr) participated in the study. Plasma products of oxidative protein damage [protein carbonyl (PCO) and nitrotyrozine (NT)], plasma oxidized LDL (oxLDL), plasma product of oxidative lipid damage [lipid hydroperoxide (LHP)] and antioxidant status of the plasma [total thiol (T-SH)] were measured. Body fat percentage, total and LDL-cholesterol concentrations were significantly higher in the hypopituitary group. Plasma PCO, NT, LHP and T-SH concentrations did not differ significantly between patients and controls. OxLDL concentration was significantly higher in the hypopituitary patients (62.4 +/- 17.8 vs 43.1 +/- 11.3 U/l, p = 0.001). In the patients, oxLDL correlated significantly with the duration of hypopituitarism (r = 0.6323, p = 0.01). In the controls, oxLDL correlated significantly with blood pressure, total and VLDL-cholesterol concentrations. Increased oxLDL concentration may indicate increased oxidative stress within the vascular compartment and may contribute to the proatherogenic state in GH-deficient hypopituitary patients independent from conventional risk factors.
authors.affiliation: Department of Internal Medicine, Division of Endocrinology, Faculty of Medicine, Istanbul University, Capa, Istanbul, Turkey. nozbey@hotmail.com
authors.forename: N
authors.initials: N
authors.lastname: Ozbey
authors.forename: A
authors.initials: A
authors.lastname: Telci
authors.forename: U
authors.initials: U
authors.lastname: Cakatay
authors.forename: A
authors.initials: A
authors.lastname: Yurci
authors.forename: S
authors.initials: S
authors.lastname: Molvalilar
mesh_terms: D000328:Adult; D000886:Anthropometry; D001823:Body Composition / Q000502:physiology; D005260:Female; D019382:Human Growth Hormone / Q000097:blood / Q000172:deficiency*; D006801:Humans; D007018:Hypopituitarism / Q000097:blood*; D008054:Lipid Peroxides / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D018709:Statistics, Nonparametric; D013438:Sulfhydryl Compounds / Q000097:blood; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: D008054:Lipid Peroxides; D008077:Lipoproteins, LDL; D013438:Sulfhydryl Compounds; C099252:oxidized low density lipoprotein; D019382:Human Growth Hormone; C002744:3-nitrotyrosine; D014443:Tyrosine