title: The solution structure of the SODD BAG domain reveals additional electrostatic interactions in the HSP70 complexes of SODD subfamily BAG domains.
abstract: The solution structure of an N-terminally extended construct of the SODD BAG domain was determined by nuclear magnetic resonance spectroscopy. A homology model of the SODD-BAG/HSP70 complex reveals additional possible interactions that are specific for the SODD subfamily of BAG domains while the overall geometry of the complex remains the same. Relaxation rate measurements show that amino acids N358-S375 of SODD which were previously assigned to its BAG domain are not structured in our construct. The SODD BAG domain is thus indeed smaller than the homologous domain in Bag1 defining a new subfamily of BAG domains.
authors.affiliation: Forschungsinstitut für Molekulare Pharmakologie, Robert-Rössle-Str. 10, D-13125 Berlin, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Brockmann
authors.forename: Dietmar
authors.initials: D
authors.lastname: Leitner
authors.forename: Dirk
authors.initials: D
authors.lastname: Labudde
authors.forename: Annette
authors.initials: A
authors.lastname: Diehl
authors.forename: Volker
authors.initials: V
authors.lastname: Sievert
authors.forename: Konrad
authors.initials: K
authors.lastname: Büssow
authors.forename: Ronald
authors.initials: R
authors.lastname: Kühne
authors.forename: Hartmut
authors.initials: H
authors.lastname: Oschkinat
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D003198:Computer Simulation; D017124:Conserved Sequence; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008962:Models, Theoretical; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D012996:Solutions; D055672:Static Electricity
chemical_list: D018840:HSP70 Heat-Shock Proteins; D012996:Solutions