Small open reading frames in 5' untranslated regions of mRnas.
Using the 5'-end sequence data from 'oligo-capped' cDNAs, we generated a representative full-length cDNA dataset for 4870 RefSeq entries, and analyzed the 5' untranslated region (UTR) of these genes. To our surprise, about half of the 4870 genes had an upstream ATG before the ATG that starts the longest open reading frame (ORF), suggesting that about half of them have small ORFs in their 5' UTR of average length of 31 amino acids. They require attention for further analysis to identify their biological role.
Yamashita
Riu
R
Laboratory of Genome Database, Human Genome Center, The Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokane-dai, Minato-ku, Tokyo 108-8639, Japan.
Suzuki
Yutaka
Y
Nakai
Kenta
K
Sugano
Sumio
S
D020121:5' Untranslated Regions / Q000235:genetics*; D018076:DNA, Complementary; D006801:Humans; D016366:Open Reading Frames / Q000235:genetics*
D020121:5' Untranslated Regions; D018076:DNA, Complementary