title: Similar molecular interactions of factor VII and factor VIIa with the tissue factor region that allosterically regulates enzyme activity.
abstract: Tissue factor (TF) binds the zymogen (VII) and activated (VIIa) forms of coagulation factor VII with high affinity. The structure determined for the sTF-VIIa complex [Banner, D. W., et al. (1996) Nature 380, 41-46] shows that all four domains of VIIa (Gla, EGF-1, EGF-2, and protease) are in contact with TF. Although a structure is not available for the TF-VII complex, the structure determined for free VII [Eigenbrot, C., et al. (2001) Structure 9, 675-682] suggests a significant conformational change for the zymogen to enzyme transition. In particular, the region of the protease domain that must contact TF has a conformation that is altered from that of VIIa, suggesting that the VII protease domain interacts with TF in a manner different from that of VIIa. To test this hypothesis, a panel of 12 single-site sTF mutants, having substitutions of residues observed to contact the proteolytic domain of VIIa, have been evaluated for binding to both zymogen VII and VIIa. Affinities were determined by surface plasmon resonance measurements using a noninterfering anti-TF monoclonal antibody to capture TF on the sensor chip surface. Dissociation constants (K(D)) measured for binding to wild-type sTF are 7.5 +/- 2.4 nM for VII and 5.1 +/- 2.3 nM for VIIa. All of the sTF mutants except S39A and E95A exhibited a significant decrease (>2-fold) in affinity for VIIa. The changes in affinity measured for VII or VIIa binding with substitution in sTF were comparable in magnitude. We conclude that the proteolytic domain of both VII and VIIa interacts with this region of sTF in a nearly identical fashion. Therefore, zymogen VII can readily adopt a VIIa-like conformation required for binding to TF.
authors.affiliation: Departments of Protein Engineering and Physiology, Genentech Inc., 1 DNA Way, South San Francisco, California 94080, USA. rk@gene.com
authors.forename: Robert F
authors.initials: RF
authors.lastname: Kelley
authors.forename: Jihong
authors.initials: J
authors.lastname: Yang
authors.forename: Charles
authors.initials: C
authors.lastname: Eigenbrot
authors.forename: Paul
authors.initials: P
authors.lastname: Moran
authors.forename: Mark
authors.initials: M
authors.lastname: Peek
authors.forename: Michael T
authors.initials: MT
authors.lastname: Lipari
authors.forename: Daniel
authors.initials: D
authors.lastname: Kirchhofer
mesh_terms: D000494:Allosteric Regulation / Q000235:genetics; D019943:Amino Acid Substitution / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D010450:Endopeptidases / Q000737:chemistry / Q000378:metabolism; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000302:isolation & purification / Q000378:metabolism; D005167:Factor VII / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D015942:Factor VIIa / Q000737:chemistry* / Q000378:metabolism; D005170:Factor X / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D016297:Mutagenesis, Site-Directed; D011485:Protein Binding / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012995:Solubility; D020349:Surface Plasmon Resonance; D013925:Thromboplastin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D004792:Enzyme Precursors; D011994:Recombinant Proteins; D005167:Factor VII; D005170:Factor X; D013925:Thromboplastin; D010450:Endopeptidases; D015942:Factor VIIa