LNCIB human full-length cDNAs collection: towards a better comprehension of the human transcriptome.
LNCIB has been producing a variety of human full-length-enriched, normalized and subtracted cDNA libraries from various cell lines and tissues in different developmental stages by using the CAP-Trapper method. By sequencing 23000 clones of these libraries we identified a pool of about 5800 good quality unique cDNAs. After BLAST analysis on Human RefSeq/Unigene databases, 1717 of these sequences remained with no or poor annotation. We show that cross-species comparative BLAST resulted as a valid tool for the annotation of orthologous genes.
Dalla
Emiliano
E
Laboratorio Nazionale CIB, Functional Genomics Group, AREA Science Park, Padriciano 99, Trieste 34012, Italy.
Verardo
Roberto
R
Lazarević
Dejan
D
Marchionni
Luigi
L
Reid
James F
JF
Bahar
Nabil
N
Klarić
Enio
E
Marcuzzi
Giacomo
G
Marzio
Riccardo
R
Belgrano
Anna
A
Licastro
Danilo
D
Schneider
Claudio
C
D016208:Databases, Factual; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D014158:Transcription, Genetic