The effects of non-weight bearing on skeletal muscle in older rats: an interrupted bout versus an uninterrupted bout.
Age-related changes in skeletal muscle, in combination with bed rest, may result in a poorer rehabilitation potential for an elderly patient. The purpose of this study was to determine the effects of non-weight bearing (hind limb unweighting [HU]) on the soleus and extensor digitorum longus (EDL) in older rats. Two non-weight bearing conditions were used: an uninterrupted bout of HU and an interrupted bout of HU. Twenty-one rats were randomly placed into 1 of 3 groups: control, interrupted HU (2 phases of 7 days of HU, separated by a 4-day weight-bearing phase) and an uninterrupted HU (18 uninterrupted days of HU). Following non-weight bearing, the soleus and EDL muscles were removed. Fiber type identification was performed by myofibrillar ATPase and cross-sectional area was determined. The findings suggest that any period of non-weight bearing leads to a decrease in muscle wet weight (19%-45%). Both type I and type II fibers of the soleus showed atrophy (decrease in cross-sectional area, 35%-44%) with an uninterrupted bout of non-weight bearing. Only the type II fibers of the soleus showed recovery with an interrupted bout of weight bearing. In the EDL, type II fibers were more affected by an uninterrupted bout of non-weight bearing (15% decrease in fiber size) compared to the type I fibers. EDL type II fibers showed more atrophy with interrupted bouts of non-weight bearing than with a single bout (a 40% compared to a 15% decrease). This study shows that initial weight bearing after an episode of non-weight bearing may be damaging to type II fibers of the EDL.
Gehrke
Alissa Guildner
AG
Program in Physical Therapy, University of Minnesota, Minneapolis, MN 55455, USA.
Krull
Margaret Sheie
MS
McDonald
Robin Shotwell
RS
Sparby
Tracy
T
Thoele
Jessica
J
Troje
Sara Willen
SW
ZumBerge
Jaime
J
Thompson
LaDora V
LV
D000251:Adenosine Triphosphatases / Q000032:analysis; D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D001510:Bed Rest / Q000009:adverse effects*; D001835:Body Weight; D004195:Disease Models, Animal; D019417:Hindlimb Suspension / Q000009:adverse effects*; D018485:Muscle Fibers, Skeletal / Q000201:enzymology / Q000473:pathology; D018482:Muscle, Skeletal; D009133:Muscular Atrophy / Q000209:etiology* / Q000473:pathology / Q000517:prevention & control* / Q000534:rehabilitation; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D013997:Time Factors; D016474:Weight-Bearing
D000251:Adenosine Triphosphatases