Camptothecin-induced apoptosis in non-small cell lung cancer is independent of cyclooxygenase expression.
Recent observations show a positive correlation between the expression of cyclooxygenase (COX), especially COX-2), and cancer development. Here we tested the hypothesis that expression of COX-2 could influence apoptosis in lung cancer cell lines. To address this question, we determined the effects of camptothecin-induced apoptosis on three lung cancer cell lines which over express COX-1 (CORL23), COX-2 (MOR-P) and neither isoform (H-460), and determine if these effects were prostaglandin mediated. We also compared the effects of non-selective and isoenzyme selective COX-2 inhibitors on camptothecin-induced apoptosis in these three cell lines. Camptothecin induced apoptosis in all three cell lines independently of COX-1 or COX-2 expression. Indomethacin, a non-selective COX inhibitor and NS398, a selective COX-2 inhibitor had no effect on camptothecin-induced apoptosis at concentrations that abolished prostaglandin production. In conclusion, these finding suggest that the COX pathway is not involved in camptothecin-induced apoptosis of non-small cell lung cancer cell lines.
Sánchez-Alcázar
J A
JA
Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide, Sevilla 41013, Spain. jasanalc@dex.upo.es
Bradbury
D A
DA
Brea-Calvo
G
G
Navas
P
P
Knox
A J
AJ
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002166:Camptothecin / Q000494:pharmacology* / Q000633:toxicity; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy*; D007213:Indomethacin / Q000494:pharmacology; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008175:Lung Neoplasms; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis / Q000235:genetics*; D011453:Prostaglandins / Q000096:biosynthesis
D000972:Antineoplastic Agents, Phytogenic; D007527:Isoenzymes; D011453:Prostaglandins; D011451:Prostaglandin-Endoperoxide Synthases; D002166:Camptothecin; D007213:Indomethacin