title: Suppression of proliferation and neurite extension of human neuroblastoma SH-SY5Y cells on immobilized Psathyrella velutina lectin.
abstract: Glycoproteins from mammalian brain tissues contain unique N-linked oligosaccharides terminating with beta-N-acetylglucosamine residues. Lectin blot analysis of membrane glycoprotein samples from human neuroblastoma SH-SY5Y cells showed that several protein bands bind to Psathylera velutina lectin (PVL), which interacts with beta-N-acetylglucosamine-terminating oligosaccharides. No lectin positive bands were detected by digestion with jack bean beta-N-acetyl-hexosaminidase or N-glycanase before incubation with the lectin, indicating that the cells contain beta-N-acetylglucosamine-terminating N-linked oligosaccharides. When cells were cultured in dishes with different concentrations of PVL, the cell proliferation was inhibited in a dose-dependent manner. Similarly, the neurite extension, which was stimulated with nerve growth factor, was also inhibited in a manner dependent on the lectin dose. Cell proliferation and neurite extension were recovered by the addition of 10 mM N-acetylglucosamine into the medium. Immunoblot analysis of the activation of mitogen-activated protein (MAP) kinases and protein kinase C revealed that phosphorylation of 42-kDa and 44-kDa MAP kinases and 80-kDa protein kinase C are inhibited when SH-SY5Y cells are cultured in PVL-coated dishes, but are restored by the addition of the haptenic sugar into the medium, indicating that MAP kinase and protein kinase C pathways are inhibited by interaction with immobilized PVL. These results indicate that beta-N-acetylglucosamine-terminating N-linked oligosaccharides expressed on neural cells can induce intracellular signals upon binding to extracellular receptors, and are important for growth regulation of neural cells.
authors.affiliation: Growth and Differentiation Research Group, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.
authors.forename: Noriaki
authors.initials: N
authors.lastname: Kitamura
authors.forename: Masahiko
authors.initials: M
authors.lastname: Ikekita
authors.forename: Satoru
authors.initials: S
authors.lastname: Hayakawa
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Funahashi
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Furukawa
mesh_terms: D000117:Acetylglucosamine / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D000363:Agaricales / Q000737:chemistry; D000818:Animals; D018929:Cell Culture Techniques; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D006801:Humans; D015151:Immunoblotting; D037102:Lectins / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D020932:Nerve Growth Factor / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects / Q000502:physiology*; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism
chemical_list: D037102:Lectins; D008562:Membrane Glycoproteins; D009844:Oligosaccharides; D020932:Nerve Growth Factor; D011493:Protein Kinase C; D020928:Mitogen-Activated Protein Kinases; D000117:Acetylglucosamine