Cytokine and adhesion molecule concentrations in blood of patients with B-cell chronic lymphocytic leukaemia with regard to disease progression.
OBJECTIVE
To examine concentrations of IL-8, E-selectin and VCAM-1 in blood plasma, culture supernatant and isolated broken lymphocytes in patients with chronic lymphocytic leukaemia (CLL), at I and III stage of the disease according to Rai's classification and in healthy individuals constituting controls. Two types of lymphocyte cultures were carried out--nonstimulated and stimulated with mitogen.


METHODS
A concentration assay of substances mentioned above was made using ready immuno-enzymatic sets of the ELISA type.


RESULTS
In all cases, plasma concentrations of IL-8, E-selectin and VCAM-1 were the highest in III stage of CLL and moderately increased in I stage comparing to control. The concentrations of IL-8 in culture supernatants and in broken lymphocytes and concentration of VCAM-1 in lymphocytes were increased in both stages of CLL. In contrast concentrations of E-selectin were decreased in lymphocytes in both types of culture. Significant decrease of E-selectin and VCAM-1 concentrations were observed in supernatants of nonstimulated cultures.


CONCLUSIONS
Significant increase of E-selectin, IL-8 and VCAM-1 concentration in blood plasma may support higher activity and suggest the higher ability of leukaemic lymphocytes to migration. Increased IL-8 concentration in isolated, broken, stimulated lymphocytes may be a characteristic feature of the biochemical processes of leukaemic lymphocytes. The lowest values of E-selectin and VCAM-1 concentrations in culture supernatants and broken lymphocytes may suggest their degradation during in culture.
Parfieńczyk
A
A
Departments of Nuclear Medicine and Haematology, Medical University of Białystok, Poland. aparf@poczta.fm
Kiersnowska-Rogowska
B
B
Rogowski
F
F
D015815:Cell Adhesion Molecules / Q000032:analysis*; D016207:Cytokines / Q000032:analysis*; D018450:Disease Progression; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood / Q000276:immunology* / Q000503:physiopathology*; D009367:Neoplasm Staging
D015815:Cell Adhesion Molecules; D016207:Cytokines