title: Pluripotency of cryopreserved blastomeres of the goldfish.
abstract: To examine the pluripotency of cryopreserved blastomeres, we transplanted them into blastula. Donor blastomeres were prepared from blastula of goldfish (Carassius auratus) and cryopreserved in liquid nitrogen for two months. Fifty-five percent and 44% of blastomeres survived after thawing. Cryopreserved blastomeres were transplanted to the blastula of triploid crucian carp (C. a. longsdorfii), which reproduces gynogenetically in nature. At four days after the operation, resultant chimeric embryos transplanted with cryopreserved blastomeres showed a survival rate (41.6%) lower than that of embryos transplanted with unfrozen blastomeres (57.1%). Transplanted blastomeres were histologically identified in various organs derived from all three germ layers. A primordial germ cell differentiated from a cryopreserved blastomere was detected in one of the 32 chimeric fish examined. These results suggest blastomeres that survive after cryopreservation retain their pluripotency and are able to differentiate into both somatic and germ cell lines.
authors.affiliation: Hokkaido Fish Hatchery, Kitakashiwagi, Eniwa, Hokkaido 061-1433, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kusuda
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Teranishi
authors.forename: Nobuhisa
authors.initials: N
authors.lastname: Koide
authors.forename: Terumi
authors.initials: T
authors.lastname: Nagai
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Arai
authors.forename: Etsuro
authors.initials: E
authors.lastname: Yamaha
mesh_terms: D000818:Animals; D001757:Blastomeres / Q000502:physiology / Q000637:transplantation*; D036703:Blastula / Q000502:physiology; D002347:Carps / Q000502:physiology; D015925:Cryopreservation; D006054:Goldfish / Q000196:embryology*; D006652:Histological Techniques; D018183:Transplantation Chimera / Q000033:anatomy & histology / Q000502:physiology*; D014183:Transplantation, Heterologous / Q000379:methods*