title: [Preliminary study on the gene expression profiles of ependymomas with cDNA array].
abstract: OBJECTIVE
  To investigate the differential gene expression of ependymomas.
  
  
  METHODS
  Four fresh samples of ependymomas and 1 of normal brain tissue were collected during operation. The extracted total RNAs were converted as (32)P tagged cDNA probes, which were then hybridized with the Atlas Human Cancer Array, producing the array based hybridization maps following the protocol provided with the kit. A set of special software was applied to the analysis and RT-PCR was performed to test the result.
  
  
  RESULTS
  In comparison with the normal brain tissue, there were 31 upregulated gene and 1 downregulated gene in ependymomas, most of which were firstly found to be differentially expressed in this kind of tumor.
  
  
  CONCLUSIONS
  The discrepancy of gene expression profiles between ependymomas and normal brain tissues is highly put through and effectively detected with cDNA array, which provides new information for the further research on the molecular mechanisms of this lesion.
authors.affiliation: Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Rong-cai
authors.initials: RC
authors.lastname: Jiang
authors.forename: Pei-yu
authors.initials: PY
authors.lastname: Pu
authors.forename: Chang-hong
authors.initials: CH
authors.lastname: Shen
authors.forename: Bao-hua
authors.initials: BH
authors.lastname: Jiao
authors.forename: Shi-zhu
authors.initials: SZ
authors.lastname: Yu
authors.forename: Chun-sheng
authors.initials: CS
authors.lastname: Kang
authors.forename: Chun-yan
authors.initials: CY
authors.lastname: Wang
authors.forename: Guang-xiu
authors.initials: GX
authors.lastname: Wang
mesh_terms: D001921:Brain / Q000378:metabolism; D001932:Brain Neoplasms / Q000235:genetics*; D004806:Ependymoma / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction