title: Mitochondria-dependent pathway is involved in heat-induced male germ cell death: lessons from mutant mice.
abstract: The signaling events leading to apoptosis can be divided into two major pathways, involving either mitochondria (intrinsic) or death receptors (extrinsic). In a recent study, we have shown the involvement of the mitochondria-dependent apoptotic pathway in heat-induced male germ cell apoptosis in the rat. In additional studies, using the gld (generalized lymphoproliferation disease) and lprcg (lymphoproliferation complementing gld) mice, which harbor loss-of-function mutations in Fas L and Fas, respectively, we have shown that heat-induced germ cell apoptosis is not blocked, thus providing evidence that the Fas signaling system is not required for heat-induced germ cell apoptosis in the testis. In the present study, we have found that the initiation of apoptosis in wild-type mice was preceded by a redistribution of Bax from a cytoplasmic to paranuclear localization in heat-susceptible germ cells. The relocation of Bax is accompanied by sequestration of ultracondensed mitochondria into paranuclear areas of apoptotic germ cells, cytosolic translocation of mitochondrial cytochrome c and DIABLO, and is associated with activation of the initiator caspase 9 and the executioner caspase 3. Similar events were also noted in both gld and lprcg mice. Taken together, these results indicate that the mitochondria-dependent pathway is the key apoptotic pathway for heat-induced male germ cell death in mice.
authors.affiliation: Division of Endocrinology, Department of Medicine, Harbor-UCLA Medical Center and Research and Education Institute, Torrance, California 90509, USA.
authors.forename: Yanira
authors.initials: Y
authors.lastname: Vera
authors.forename: Maruja
authors.initials: M
authors.lastname: Diaz-Romero
authors.forename: Susana
authors.initials: S
authors.lastname: Rodriguez
authors.forename: Yanhe
authors.initials: Y
authors.lastname: Lue
authors.forename: Christina
authors.initials: C
authors.lastname: Wang
authors.forename: Ronald S
authors.initials: RS
authors.lastname: Swerdloff
authors.forename: Amiya P
authors.initials: AP
authors.lastname: Sinha Hikim
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism; D053148:Caspase 3; D053453:Caspase 9; D020169:Caspases / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D045304:Cytochromes c / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004789:Enzyme Activation; D053222:Fas Ligand Protein; D006358:Hot Temperature; D008232:Lymphoproliferative Disorders / Q000235:genetics; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics; D051379:Mice; D019463:Mice, Inbred MRL lpr; D008815:Mice, Inbred Strains / Q000235:genetics; D008928:Mitochondria / Q000378:metabolism / Q000502:physiology*; D024101:Mitochondrial Proteins / Q000378:metabolism; D009154:Mutation; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D013094:Spermatozoa / Q000502:physiology*; D014018:Tissue Distribution; D051028:bcl-2-Associated X Protein; D019014:fas Receptor / Q000235:genetics
chemical_list: D051017:Apoptosis Regulatory Proteins; C494827:Bax protein, mouse; D002352:Carrier Proteins; C499605:Diablo protein, mouse; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; C505662:Faslg protein, rat; D008562:Membrane Glycoproteins; D024101:Mitochondrial Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D019014:fas Receptor; D045304:Cytochromes c; C505409:Casp3 protein, mouse; C505410:Casp3 protein, rat; C506434:Casp9 protein, mouse; C506435:Casp9 protein, rat; D053148:Caspase 3; D053453:Caspase 9; D020169:Caspases