title: [Significance of serological markers and virological marker for hepatitis E in rhesus monkey model].
abstract: OBJECTIVE
  To evaluate the serological markers and biological marker in the diagnosis of hepatitis E infection in a rhesus monkey model.
  
  
  METHODS
  86 rhesus monkeys had been infected with different doses of HEV. Hence, they were taken sequential blood samples at intervals up to 86 weeks for 4 hepatitis E virus (HEV) specific antibody assays (E2-IgM, E2-IgG, GL-IgG, and YES-IgG), and nucleic acid assay.
  
  
  RESULTS
  All the animals produced E2-IgG and all but one also produced E2-IgM and excreted the virus in stool, whereas positive rate of GL-IgG and YES IgG were low and correlated with virus level. Hepatitis occurred over a period of 4 weeks (between 3 an 7 weeks) after infection. Virological marker occurred mainly during incubation period and declined rapidly after onset of hepatitis. Seroconversion of E2-IgM occurred before onset of hepatitis in 70% monkeys and declined rapidly up to 50% of peak value after 4 weeks. E2-IgM seroconversion was closely paralleled by E2-IgG; however, E2-IgG persisted in all animals for the entire duration of experiment of up to 86 weeks. Production of GL-IgG and YES-IgG was delayed by one week after the E2 antibodies, these antibodies showed a transient occurrence and seroprevalence declined to 50% of the peak value over a period of 12 weeks.
  
  
  CONCLUSIONS
  E2-IgM might be used as a suitable acute hepatitis E marker, and E2-IgG as a suitable epidemiological marker. The seroconversion or titer elevation of GL-IgG and YES-IgG antibodies probably used to confirm the infection. The viral markers are optional for early diagnosis.
authors.affiliation: The Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Sheng-xiang
authors.initials: SX
authors.lastname: Ge
authors.forename: Guo-yong
authors.initials: GY
authors.lastname: Huang
authors.forename: Shao-wei
authors.initials: SW
authors.lastname: Li
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: He
authors.forename: Ying-bing
authors.initials: YB
authors.lastname: Wang
authors.forename: Ying-jie
authors.initials: YJ
authors.lastname: Zheng
authors.forename: Ying
authors.initials: Y
authors.lastname: Gu
authors.forename: Mun-hon
authors.initials: MH
authors.lastname: Ng
authors.forename: Ning-shao
authors.initials: NS
authors.lastname: Xia
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D015415:Biomarkers; D005838:Genotype; D006508:Hepatitis Antibodies / Q000097:blood*; D016751:Hepatitis E / Q000175:diagnosis*; D016752:Hepatitis E virus / Q000145:classification / Q000235:genetics / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008253:Macaca mulatta
chemical_list: D015415:Biomarkers; D006508:Hepatitis Antibodies; D007075:Immunoglobulin M; D007073:Immunoglobulin E; D000410:Alanine Transaminase