In vitro anti-inflammatory activity of panduratin A isolated from Kaempferia pandurata in RAW264.7 cells.
An active compound identified as panduratin A was isolated from a methanol extract of Kaempferia pandurata (Zingiberaceae). We examined the effect of panduratin A on nitric oxide (NO) and prostaglandin E (2) (PGE (2)) production induced by lipopolysaccharide (LPS) in RAW264.7 cells. Modulations of iNOS and COX-2 enzyme expression were evaluated by Western blotting. Panduratin A strongly inhibited both NO (IC (50): 0.175 microM) and PGE (2) (IC (50): 0.0195 microM) production and suppressed both iNOS and COX-2 enzyme expression without any appreciable cytotoxic effect on RAW264.7 cells in a dose-dependent manner. Panduratin A also suppressed the phosphorylation of inhibitor kappaBalpha (IkappaBalpha) and degradation of IkappaBalpha associated with nuclear factor kappaB (NF-kappaB) activation. Furthermore, panduratin A inhibited LPS-induced NF-kappaB transcriptional activity in a dose-dependent manner. These results suggest that panduratin A could exert its inhibitory effects on the production of NO and PGE (2) through the suppression of NF-kappaB activation, indicating its potential for use as an anti-inflammatory agent.
Yun
Jung-Mi
JM
Bioproducts Research Center, Yonsei University, Seoul, Korea.
Kwon
Hoonjeong
H
Hwang
Jae-Kwan
JK
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology*; D015153:Blotting, Western; D047188:Chalcones; D051546:Cyclooxygenase 2; D015232:Dinoprostone / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D020128:Inhibitory Concentration 50; D007527:Isoenzymes / Q000187:drug effects / Q000378:metabolism; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000187:drug effects / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D011451:Prostaglandin-Endoperoxide Synthases / Q000187:drug effects / Q000378:metabolism; D030521:Zingiberaceae
D000894:Anti-Inflammatory Agents, Non-Steroidal; D047188:Chalcones; D007527:Isoenzymes; D008070:Lipopolysaccharides; D010936:Plant Extracts; C482884:panduratin A; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone