Identification and characterization of Se-methyl selenomethionine in Brassica juncea roots.
The present work shows the identification and characterization of Se-methyl selenomethionine (SeMMet) as an important Se species in Brassica juncea roots when grown in the presence of Se-methionine (SeMet) as the Se source. SeMMet was isolated by liquid chromatography employing two different liquid chromatographic mechanisms: reversed-phase ion-pairing using heptafluorobutyric acid as counter ion and cation exchange using a pyridinium formate gradient (pH 3). Inductively coupled plasma mass spectrometry was used for the detection of Se. SeMMet was characterized by electrospray quadrupole time-of-flight MS in both a synthesized standard and in the roots extract using collision-induced dissociation of the selected ion. Preliminary evidence suggests that Brassica juncea may also produce dimethylselenonium propionate, although to a much lesser extent.
Grant
Tyre D
TD
Department of Chemistry, University of Cincinnati, PO Box 0172, Cincinnati, OH 45221-0172, USA.
Montes-Bayón
María
M
LeDuc
Danika
D
Fricke
Michael W
MW
Terry
Norman
N
Caruso
Joseph A
JA
D002138:Calibration; D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D009149:Mustard Plant / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry*; D012015:Reference Standards; D012643:Selenium / Q000032:analysis* / Q000737:chemistry; D012645:Selenomethionine / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
D010936:Plant Extracts; D012645:Selenomethionine; D012643:Selenium