title: In vitro lesion repair by human amnion epithelial and mesenchymal cells.
abstract: OBJECTIVE
  The purpose of this study was to compare wound healing by human amnion epithelial and mesenchymal cells from preterm and term placenta with the use of an in vitro lesion repair assay.
  
  
  METHODS
  Lesions were created in confluent monolayers of amnion epithelial and mesenchymal cells from preterm and term placentas. The repair was monitored by the measurement of the lesion area and the response to potential stimulants (platelet-derived growth factor, tumor necrosis factor-alpha, fibrinogen, and phorbol myristate acetate). Cell proliferation was detected with 5-bromodeoxyuridine staining.
  
  
  RESULTS
  Lesion repair was complete within 40 hours in control epithelial cultures from preterm and term placenta but incomplete in mesenchymal cultures (preterm cells, 80%; term cells, 40%). Platelet-derived growth factor, tumor necrosis factor-alpha, fibrinogen, and phorbol myristate acetate did not accelerate repair in either cell type.
  
  
  CONCLUSIONS
  An in vitro lesion repair assay revealed the differences in lesion repair capacity between amnion epithelial and mesenchymal cells and between mesenchymal cells from preterm and term placenta.
authors.affiliation: Department of Obstetrics, Unit of Perinatal Physiology, Zurich University Hospital, Switzerland.
authors.forename: Grozdana
authors.initials: G
authors.lastname: Bilic
authors.forename: Nicole
authors.initials: N
authors.lastname: Ochsenbein-Kölble
authors.forename: Heike
authors.initials: H
authors.lastname: Hall
authors.forename: Renate
authors.initials: R
authors.lastname: Huch
authors.forename: Roland
authors.initials: R
authors.lastname: Zimmermann
mesh_terms: D000650:Amnion / Q000293:injuries* / Q000503:physiopathology*; D002455:Cell Division; D002478:Cells, Cultured; D004847:Epithelial Cells; D005260:Female; D005340:Fibrinogen / Q000494:pharmacology; D005865:Gestational Age; D006801:Humans; D008648:Mesoderm; D010920:Placenta; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D012421:Rupture / Q000473:pathology / Q000503:physiopathology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects
chemical_list: D010982:Platelet-Derived Growth Factor; D014409:Tumor Necrosis Factor-alpha; D005340:Fibrinogen; D013755:Tetradecanoylphorbol Acetate