Apoptosis of human gastric adenocarcinoma cells induced by beta-ionone.
OBJECTIVE
To investigate the effect of beta-ionone on the growth and apoptosis of gastric adenocarcinoma cell line SGC-7901.


METHODS
Using MTT, fluorescence dye (Hoechst-33258), transmission electron microscopy and the TUNEL assay, we examined growth and apoptosis of SGC-7901 cells treated with beta-ionone at various concentrations (i.e. 25, 50, 100 and 200 micromol/L) for 24 h, 48 h.


RESULTS
The growth of SGC-7901 cells was inhibited by beta-ionone. Seven days after treatment with beta-ionone at four concentrations, the inhibition rates were 12.04%, 30.59%, 78.25% and 94.15%, respectively. The IC(50) value of beta-ionone for SGC-7901 cells was estimated to be 89 micromol/L. The apoptotic morphology was demonstrated in SGC-7901 cells treated with beta-ionone by Hoechst-33258 staining and electron microscopy. Apoptosis was also shown in beta-ionone-treated SGC-7901 cells by the TUNEL assay.


CONCLUSIONS
beta-ionone can inhibit cell proliferation and induce apoptosis of SGC-7901 cells. However, the mechanism needs to be further investigated.
Liu
Jia-Ren
JR
Public Health College, Harbin Medical University, Harbin 15001, Heilongjiang Province, China. jiarl@ems.hrbmu.edu.cn
Chen
Bing-Qing
BQ
Yang
Bao-Feng
BF
Dong
Hong-Wei
HW
Sun
Chang-Hao
CH
Wang
Qi
Q
Song
Guo
G
Song
You-Qiang
YQ
D000230:Adenocarcinoma / Q000503:physiopathology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D006801:Humans; D045792:Norisoprenoids / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000503:physiopathology*
D045792:Norisoprenoids; C008157:beta-ionone