Evaluation of the nitric oxide radical scavenging activity of manganese complexes of curcumin and its derivative.
Curcumin manganese complex (CpCpx) and diacetylcurcumin manganese complex (AcylCpCpx) were determined as to their effect on the nitric oxide (NO) radical scavenging in vitro method using a sodium nitroprusside generating NO system compared with their parent compound and astaxanthin, an extreme antioxidant. All compounds effectively reduced the generation of NO radicals in a dose dependent manner. They exhibited strong NO radical scavenging activity with low IC(50) values. The IC(50) values of curcumin, diacetylcurcumin, CpCpx and AcylCpCpx obtained are 20.39+/-4.10 microM, 28.76+/-1.48 microM, 9.79+/-1.50 microM and 8.09+/-0.99 microM, respectively. CpCpx and AcylCpCpx show greater NO radical scavenging than their parent compounds, curcumin and acetylcurcumin, respectively. However, the IC(50) values of curcumin and related compounds were found to be less than astaxanthin, an extreme antioxidant, with the lower IC(50) value of 3.42+/-0.50 microM.
Sumanont
Yaowared
Y
Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan.
Murakami
Yukihisa
Y
Tohda
Michihisa
M
Vajragupta
Opa
O
Matsumoto
Kinzo
K
Watanabe
Hiroshi
H
D003474:Curcumin / Q000031:analogs & derivatives* / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry*; D008345:Manganese / Q000737:chemistry*; D009569:Nitric Oxide / Q000737:chemistry*; D009599:Nitroprusside / Q000737:chemistry; D011794:Quercetin / Q000737:chemistry; D013329:Structure-Activity Relationship; D024341:Xanthophylls; D019207:beta Carotene / Q000031:analogs & derivatives* / Q000737:chemistry
D016166:Free Radical Scavengers; D024341:Xanthophylls; D019207:beta Carotene; D009599:Nitroprusside; D009569:Nitric Oxide; D008345:Manganese; C493686:diacetylcurcumin; C005948:astaxanthine; D011794:Quercetin; D003474:Curcumin