Thirteen-week repeated dose toxicity study of wormwood (Artemisia absinthium) extract in rats.
Wormwood, Artemisia absinthium, is a very bitter plant, and its extract has been used as food additives such as seasonings for food and drinks. A 13-week repeated dose toxicity study of wormwood extract was performed in both sexes of Wistar Hannover (GALAS) rats. Rats were divided into 4 groups consisting of 10 males and 10 females each, and were given water containing 0, 0.125, 0.5, or 2% wormwood extract. All rats had survived at the end of the study, and no changes indicating obvious toxicities that are attributable to the treatment of wormwood extract were observed in the body weights, hematological and serum biochemical examinations, organ weights, and histopathological examinations. Based on the results of the present study, the NOAEL (no-observed-adverse-effect-level) of wormwood extract of Wistar Hannover rats was estimated to be 2% (equivalent to 1.27 g/kg/day in males and 2.06 g/kg/day in females) or more.
Muto
Tomoko
T
Laboratory of Veterinary Pathology, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.
Watanabe
Takao
T
Okamura
Miwa
M
Moto
Mitsuyoshi
M
Kashida
Yoko
Y
Mitsumori
Kunitoshi
K
D000284:Administration, Oral; D000818:Animals; D031171:Artemisia absinthium / Q000633:toxicity*; D005260:Female; D005503:Food Additives / Q000633:toxicity; D008297:Male; D019055:No-Observed-Adverse-Effect Level; D010936:Plant Extracts / Q000633:toxicity; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D018675:Toxicity Tests; D014867:Water / Q000008:administration & dosage
D005503:Food Additives; D010936:Plant Extracts; D014867:Water