Hydrogen sulfide induces calcium waves in astrocytes.
Hydrogen sulfide (H2S) modifies hippocampal long-term potentiation (LTP) and functions as a neuromodulator. Here, we show that H2S increases intracellular Ca2+ and induces Ca2+ waves in primary cultures of astrocytes as well as hippocampal slices. H2S increases the influx of Ca2+ and to a lesser extent causes the release from intracellular Ca2+ stores. Ca2+ waves induced by neuronal excitation as well as responses to exogenously applied H2S are potently blocked by La3+ and Gd3+, inhibitors of Ca2+ channels. These observations suggest that H2S induces Ca2+ waves that propagate to neighboring astrocytes.
Nagai
Yasuo
Y
National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, Japan.
Tsugane
Mamiko
M
Oka
Jun-Ichiro
J
Kimura
Hideo
H
D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020013:Calcium Signaling / Q000187:drug effects*; D002478:Cells, Cultured / Q000187:drug effects; D018920:Coculture Techniques; D045604:Extracellular Fluid / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D005682:Gadolinium / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D007424:Intracellular Fluid / Q000378:metabolism; D007811:Lanthanum / Q000494:pharmacology; D017774:Long-Term Potentiation / Q000187:drug effects; D016202:N-Methylaspartate / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects; D009928:Organ Specificity; D009930:Organic Chemicals; D051381:Rats; D017207:Rats, Sprague-Dawley; D012430:Ruthenium Red / Q000494:pharmacology; D026941:Sodium Channel Blockers / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology
D002121:Calcium Channel Blockers; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D026941:Sodium Channel Blockers; D012430:Ruthenium Red; C071336:calcium green; D013779:Tetrodotoxin; D016202:N-Methylaspartate; D007811:Lanthanum; D005682:Gadolinium; D002118:Calcium; D006862:Hydrogen Sulfide