title: Gene expression pattern and immunoreactive protein localization of LGR7 receptor in human endometrium throughout the menstrual cycle.
abstract: Relaxin (RLX) is a pregnancy-associated polypeptide hormone. In non-pregnant women, the peak of circulating relaxin coincides with the window of endometrial receptivity and both in vivo and in vitro experiments showed that it plays a role in the decidualization process. Recently, two receptors, LGR7 and LGR8, have been identified as high affinity receptors for relaxin. Here we describe LGR7 mRNA and protein expression in human endometrium using semi-quantitative and quantitative fluorescent PCR (Q-PCR) and immunohistochemical analyses. Three different experimental designs were used. First, endometrial biopsies from five different phases of the menstrual cycle were analysed. Secondly, we assessed the early luteal phase in more detail. Finally we analysed the expression at LH+2 (2 days after the natural LH surge, pre-receptive endometrium) versus LH+7 (receptive endometrium) within the same menstrual cycle from the same patient to avoid inter-cycle or inter-person variations in gene expression. Our results indicate that there is no consistent regulation of LGR7 mRNA expression, neither during the menstrual cycle nor during the early-mid-luteal phase. In general, we observed a large degree of variation in LGR7 mRNA expression levels between patients. LGR7 immunoreactive protein was identified in all stages of the menstrual cycle. LGR7 protein was localized in both the epithelial and the stromal compartments, except for the mid-luteal phase when the expression was restricted to the endometrial epithelium. We conclude that no consistent regulation of LGR7 mRNA expression can be detected in human endometrium during the menstrual cycle.
authors.affiliation: Foundation of the Instituto Valenciano de Infertilidad, Valencia University School of Medicine, Valencia, Spain.
authors.forename: José Juan
authors.initials: JJ
authors.lastname: Luna
authors.forename: Anne
authors.initials: A
authors.lastname: Riesewijk
authors.forename: José A
authors.initials: JA
authors.lastname: Horcajadas
authors.forename: Roselin de
authors.initials: Rd
authors.lastname: Van Os Rd
authors.forename: Francisco
authors.initials: F
authors.lastname: Domínguez
authors.forename: Sietse
authors.initials: S
authors.lastname: Mosselman
authors.forename: Antonio
authors.initials: A
authors.lastname: Pellicer
authors.forename: Carlos
authors.initials: C
authors.lastname: Simón
mesh_terms: D002460:Cell Line; D004717:Endometrium / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism*; D005260:Female; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D007120:Immunochemistry; D008183:Luteal Phase / Q000378:metabolism; D008565:Membrane Proteins / Q000032:analysis* / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D008597:Menstrual Cycle / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000032:analysis* / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D018000:Receptors, Peptide; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D008565:Membrane Proteins; D012333:RNA, Messenger; C417543:RXFP1 protein, human; D043562:Receptors, G-Protein-Coupled; D018000:Receptors, Peptide