Comparative analysis of genetic diversity and expression of amoA in wastewater treatment processes.
The genetic diversity and expression of amoA of autotrophic ammonia oxidizers in wastewater treatment processes were investigated by RT-PCR and denaturing gradient gel electrophoresis (DGGE) in order to identify active components of ammonia-oxidizer populations in a such processes. Ammonia oxidizers, evidenced by the presence of amoA mRNA, were regarded as metabolically active. The DGGE profiles derived from amoA mRNA and from its gene, which were amplified by RT-PCR or PCR using samples collected from a bench-scale reactor treating high concentration of inorganic ammonia, were similar. In contrast, RNA and DNA-derived DGGE profiles from three domestic wastewater treatment facilities were different from each other. These data indicate that the dominant ammonia oxidizers in the bench-scale reactor exhibited ammonia-oxidizing activity, whereas some ammonia oxidizers in the domestic wastewater treatment facilities apparently did not express high levels of amoA mRNA.
Ebie
Y
Y
Institute of Applied Biochemistry, University of Tsukuba, 305-0006 Ibaraki, Japan. ebie.yoshitaka@nies.go.jp
Noda
N
N
Miura
H
H
Matsumura
M
M
Tsuneda
S
S
Hirata
A
A
Inamori
Y
Y
D000641:Ammonia / Q000378:metabolism*; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D043370:Nitrosomonas europaea / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D010088:Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics; D010802:Phylogeny; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D018508:Water Purification / Q000379:methods
D012333:RNA, Messenger; D012722:Sewage; D000641:Ammonia; D010088:Oxidoreductases; C045582:ammonia monooxygenase