Solution conformations of cyclosporins and magnesium-cyclosporin complexes determined by vibrational circular dichroism.
Vibrational circular dichroism (VCD) spectroscopy was used to investigate the solution conformations of cyclosporins A, C, D, G, and H in CDCl(3), in the amide I and NH/OH-stretching regions, and their corresponding magnesium complexes in CD(3)CN, in the amide I region. VCD spectra are sensitive to the chiral arrangement of Cdbond;O and NH bonds in this cyclic undecapeptide. Calculations of molecular geometries, as well as IR and VCD intensities of model cyclosporin fragments that include the intramolecular hydrogen bonds of the crystal conformations of cyclosporins A and H (CsA and CsH), were carried out at the density functional theory (DFT; BPW91 functional/6-31G* basis set) level. The good agreement between IR and VCD spectra from experiment and DFT calculations provides evidence that the crystal conformation of CsA is dominant in CDCl(3) solution; CsH, however, assumes both an intramolecularly hydrogen-bonded crystal conformation and more open forms in solution. Comparisons of the experimental and calculated VCD spectra in the NH/OH-stretching region of the noncomplexed cyclosporins indicate that conformers with both free and hydrogen-bonded NH and OH groups are present in solution. Differences between the IR and VCD spectra for the metal-free and magnesium-complexed cyclosporins are indicative of strong interactions between cyclosporins and magnesium ions.
Bodack
Louise A
LA
Department of Chemistry, Syracuse University, Syracuse, NY 13244-4100, USA.
Freedman
Teresa B
TB
Chowdhry
Babur Z
BZ
Nafie
Laurence A
LA
D002942:Circular Dichroism; D003524:Cyclosporins / Q000737:chemistry*; D006801:Humans; D008274:Magnesium / Q000737:chemistry*; D008958:Models, Molecular; D009942:Organometallic Compounds / Q000737:chemistry; D011487:Protein Conformation; D013055:Spectrophotometry, Infrared
D003524:Cyclosporins; D009942:Organometallic Compounds; D008274:Magnesium