title: Challenges and opportunities in the analysis of raffinose oligosaccharides, pentosans, phytate, and glucosinolates.
abstract: In this paper, the status of the analytical technologies for assaying animal antinutritional compounds, such as raffinose oligosaccharides, pentosans, phytic acid, and glucosinolates, is reviewed in terms of selectivity, sensitivity, and sample throughput. The implementation of simplified sample preparation schemes, use of novel separation approaches, and alternate detector technologies are discussed. The challenges and opportunities posed by these assays are highlighted along with the recommendations for best analytical practices.
authors.affiliation: Crop Analytics, Monsanto Company, St. Louis, MO 63167, USA. dvvinj@monsanto.com
authors.forename: D V
authors.initials: DV
authors.lastname: Vinjamoori
authors.forename: J R
authors.initials: JR
authors.lastname: Byrum
authors.forename: T
authors.initials: T
authors.lastname: Hayes
authors.forename: P K
authors.initials: PK
authors.lastname: Das
mesh_terms: D000821:Animal Feed / Q000032:analysis* / Q000592:standards; D000818:Animals; D002845:Chromatography / Q000379:methods / Q000662:veterinary; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000662:veterinary; D003124:Colorimetry / Q000662:veterinary; D005961:Glucosinolates / Q000032:analysis*; D009753:Nutritive Value; D009844:Oligosaccharides / Q000032:analysis*; D010833:Phytic Acid / Q000032:analysis*; D011887:Raffinose / Q000032:analysis; D012680:Sensitivity and Specificity
chemical_list: D005961:Glucosinolates; D009844:Oligosaccharides; D010833:Phytic Acid; D011887:Raffinose