title: Possible role of dentin matrix in region-specific deposition of cellular and acellular extrinsic fibre cementum.
abstract: The mechanism whereby a region-specific deposition of the two types of cementum (cellular cementum and acellular extrinsic fibre cementum) is regulated on the growing root surface was tested using bisphosphonate-affected teeth of young rats and guinea pigs. The animals were injected subcutaneously with 8 or 10 mg P x kg body weight(-1) x day(-1) of 1-hydroxyethylidene-1,1-bisphosphonate (HEBP) for 1 or 2 weeks. In rat molars, HEBP prevented mineralization of newly formed root dentin matrix and totally inhibited de novo deposition of acellular extrinsic fibre cementum. Instead, thick cellular cementum was induced on the non-mineralized root dentin surface, irrespective of the position of the root. In both animals, cellular cementum was also induced on the non-mineralized surface of root analogue dentin in HEBP-affected incisors, where only acellular extrinsic fibre cementum is deposited under normal conditions. In normal rat molars, dentin sialoprotein (DSP) was concentrated along the dentin-cellular cementum border, but not that of dentin and acellular extrinsic fibre cementum. In HEBP-affected rat incisors, DSP was shown to penetrate through the non-mineralized dentin into the surrounding tissues, but not through the mineralized portions. These data suggest that, at the site of cellular cementum formation, putative inducing factors for cellular cementum might diffuse into the periodontal space through the newly deposited mantle dentin matrix before it is mineralized. At earlier stages of root formation, mantle dentin might mineralize more promptly not to allow such diffusion. The timing of mineralization of mantle dentin matrix might be the key determinant of the types of the cementum deposited on the growing root surface.
authors.affiliation: Division of Biostructural Science, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan. takanoy.bss@tmd.ac.jp
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Takano
authors.forename: Hideo
authors.initials: H
authors.lastname: Sakai
authors.forename: Eiko
authors.initials: E
authors.lastname: Watanabe
authors.forename: Noriko
authors.initials: N
authors.lastname: Ideguchi-Ohma
authors.forename: Chantha K
authors.initials: CK
authors.lastname: Jayawardena
authors.forename: Kazumi
authors.initials: K
authors.lastname: Arai
authors.forename: Yukiyo
authors.initials: Y
authors.lastname: Asawa
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Nakano
authors.forename: Yoko
authors.initials: Y
authors.lastname: Shuda
authors.forename: Yujiro
authors.initials: Y
authors.lastname: Sakamoto
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Terashima
mesh_terms: D000818:Animals; D025021:Cementogenesis; D003739:Dental Cementum / Q000378:metabolism*; D003804:Dentin / Q000737:chemistry / Q000378:metabolism*; D004164:Diphosphonates / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006168:Guinea Pigs; D007180:Incisor / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D008297:Male; D008963:Molar / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D014092:Tooth Root
chemical_list: D004164:Diphosphonates