Destruction of Cucumber green mottle mosaic virus by heat treatment and rapid detection of virus inactivation by RT-PCR.
Heat treatment is commonly used to control viral contamination of seeds. To study virus inactivation, virus was purified from seeds contaminated with Cucumber green mottle mosaic virus (CGMMV) after various heat treatments. CGMMV particles were observed to be physically disrupted by high temperature. Analysis of viral RNA revealed that the 5' and 3' termini of the genome were protected whereas regions between 2-2.5, 3.2-3.7 and 4-4.8 kb from the 5' terminus were not. Heat inactivation of virus on seeds was confirmed by RT-PCR using primers for a heat-sensitive region. The RT-PCR approach developed here may prove preferable to time- and labor-intensive bioassays for assessing virus heat inactivation.
Kim
Sang-Min
SM
School of Agricultural Biotechnology, Center for Plant Molecular Genetics and Breeding Research, Seoul National University, Seoul 151-742, Korea.
Nam
Sang-Hyun
SH
Lee
Jung-Myung
JM
Yim
Kyu-Ock
KO
Kim
Kook-Hyung
KH
D018553:Cucumis sativus / Q000821:virology*; D004203:Disinfection / Q000379:methods*; D006358:Hot Temperature; D008854:Microscopy, Electron; D012639:Seeds / Q000821:virology; D017898:Tobamovirus / Q000235:genetics / Q000378:metabolism*