N-nitrosation and denitrosation on lysine residues of histones.
Increased immunoactivity of (-nitrosolysine was detected in Western blot in peroxynitrite-reacted histones H2A, H3 and H4. The N-nitrosation may be reversible as indicated by the decreased immunoactivity via either subsequent incubation with reduced glutathione or 48-hour dialysis and 48-hour storage at 4 degrees C. Nonetheless, the biological significance of N-nitrosation of protein lysine is yet to be proven.
Kuo
Wu-Nan
WN
Division of Science and Mathematics, Bethune-Cookman College, Daytona Beach, Florida 32114, USA. kuow@cookman.edu
Ivy
Dynisha
D
Robinson
Marvin J
MJ
D000818:Animals; D015153:Blotting, Western; D006657:Histones / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism*; D015538:Nitrosation; D011817:Rabbits
D006657:Histones; D008239:Lysine