title: Electrothermal atomic absorption spectrometric-determination of cobalt in human serum and urine.
abstract: Problems and possibilities of the determination of Co in serum and urine samples by electrothermal atomic absorption spectrometry (ETAAS) are described. Optimal instrumental parameters as well as a suitable atomizer, calibration procedure and hydrogen peroxide as modifier are proposed for direct ETAAS measurement of Co in serum and urine. The detection limit achieved was 0.1 microg L(-1) for both matrices and relative standard deviations varied in the range 5-20% depending on the Co concentration in the sample. Validity of the method was verified by the analyses of standard reference materials. For serum samples with a Co content lower than the detection limit, a separation and preconcentration procedure based on liquid/liquid extraction is suggested prior to determination of Co in the organic phase by ETAAS. This procedure permits determination of 0.02 microg L(-1) Co in serum samples with a relative standard deviation of 10-18%.
authors.affiliation: Institute of Preventive Medical Care and Toxicology, Military Health Institution Center, 1000 Skopje, Macedonia.
authors.forename: Nadica
authors.initials: N
authors.lastname: Todorovska
authors.forename: Irina
authors.initials: I
authors.lastname: Karadjova
authors.forename: Sonja
authors.initials: S
authors.lastname: Arpadjan
authors.forename: Trajce
authors.initials: T
authors.lastname: Stafilov
mesh_terms: D002138:Calibration; D003035:Cobalt / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D007202:Indicators and Reagents; D011392:Proline / Q000031:analogs & derivatives* / Q000737:chemistry; D011786:Quality Control; D012044:Regression Analysis; D013054:Spectrophotometry, Atomic; D013696:Temperature; D013861:Thiocyanates
chemical_list: C097299:4-aminopyrrolidine-2,4-dicarboxylic acid; D007202:Indicators and Reagents; D013861:Thiocyanates; D003035:Cobalt; D011392:Proline; C026976:thiocyanic acid