title: Interaction of checkpoint kinase 1 and the X-linked inhibitor of apoptosis during mitosis.
abstract: We report here that the checkpoint kinase Chk1 and the inhibitor of apoptosis protein (IAP) family member XIAP can be found in a complex in association with condensed chromosomes aligned at the metaphase plate during mitosis. The interaction between Chk1 and XIAP was transient and followed the breakdown of the nuclear envelope. Chk1 and XIAP also formed a complex in vitro and in coimmunoprecipitation experiments. The interaction between Chk1 and the BIR3 domain of XIAP in vitro required an N-terminal sequence in Chk1 that is identical to the BIR-binding motif at the N-terminus of HID. An interaction of Chk1 and XIAP may imply a mechanism of coupling between the regulatory networks that control cell cycle progression and apoptosis during mitosis.
authors.affiliation: Buck Institute for Age Research, 8001 Redwood Blvd., Novato, CA 94945, USA.
authors.forename: V
authors.initials: V
authors.lastname: Galvan
authors.forename: A V
authors.initials: AV
authors.lastname: Kurakin
authors.forename: D E
authors.initials: DE
authors.lastname: Bredesen
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects; D000071877:Checkpoint Kinase 1; D004847:Epithelial Cells / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D005982:Glutathione Transferase / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007739:L Cells; D051379:Mice; D008938:Mitosis; D009154:Mutation; D015739:Nocodazole / Q000494:pharmacology; D011233:Precipitin Tests; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D051636:X-Linked Inhibitor of Apoptosis Protein
chemical_list: D011506:Proteins; D011993:Recombinant Fusion Proteins; D051636:X-Linked Inhibitor of Apoptosis Protein; C496866:XIAP protein, human; D005982:Glutathione Transferase; D011494:Protein Kinases; C000605867:CHEK1 protein, human; D000071877:Checkpoint Kinase 1; C000605869:Chek1 protein, mouse; D015739:Nocodazole