title: A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay.
abstract: Reliable quantitative evaluation of molecular pathways is critical for both drug discovery and treatment monitoring. We have modified the CAM assay to quantitatively measure vascular density, endothelial proliferation, and changes in protein expression in response to anti-angiogenic and pro-angiogenic agents. This improved CAM assay can correlate changes in vascular density with changes seen on a molecular level. We expect that these described modifications will result in a single in vivo assay system, which will improve the ability to investigate molecular mechanisms underlying the angiogenic response.
authors.affiliation: Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA. Steven_Libutti@nih.gov
authors.forename: Walter J
authors.initials: WJ
authors.lastname: Miller
authors.forename: Mark L
authors.initials: ML
authors.lastname: Kayton
authors.forename: Angela
authors.initials: A
authors.lastname: Patton
authors.forename: Sarah
authors.initials: S
authors.lastname: O'Connor
authors.forename: Mei
authors.initials: M
authors.lastname: He
authors.forename: Huan
authors.initials: H
authors.lastname: Vu
authors.forename: Galina
authors.initials: G
authors.lastname: Baibakov
authors.forename: Dominique
authors.initials: D
authors.lastname: Lorang
authors.forename: Vladimir
authors.initials: V
authors.lastname: Knezevic
authors.forename: Elise
authors.initials: E
authors.lastname: Kohn
authors.forename: H Richard
authors.initials: HR
authors.lastname: Alexander
authors.forename: David
authors.initials: D
authors.lastname: Stirling
authors.forename: Faribourz
authors.initials: F
authors.lastname: Payvandi
authors.forename: George W
authors.initials: GW
authors.lastname: Muller
authors.forename: Steven K
authors.initials: SK
authors.lastname: Libutti