title: Alterations in the expression of lipid and mechano-gated two-pore domain potassium channel genes in rat brain following chronic cerebral ischemia.
abstract: To determine the gene expression level of the lipid-sensitive mechano-gated 2P domain K(+) channels in rat cortex and hippocampus after chronic cerebral ischemia, TREK-1, TREK-2 and TRAAK were detected in rats at 3 and 30 days after permanent bilateral carotid artery ligation (BCAL). By using reverse transcription-polymerase chain reaction, we found that TREK-1 and TREK-2 mRNA had no obvious changes in cortex after ischemia, while TRAAK mRNA was significantly increased by 70% after 30 days. In hippocampus, the gene expressions of TREK-1 and TRAAK were increased markedly at 3 days (97% and 87%, respectively) and 30 days (63% and 47%, respectively) after ischemia. However, TREK-2 gene expression level had no change. In-situ hybridization results also showed that TREK-1 gene expressing-positive neurons were increased significantly in cortex and hippocampus after permanent BCAL for 3 and 30 days. These molecular studies provide evidence for an involvement of the lipid-sensitive mechano-gated 2P domain K(+) channels in the BCAL model, which might have neuroprotective effects in cerebral ischemia.
authors.affiliation: Department of Pharmacology, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Xiannongtan Street, 100050, Beijing, China. wangxl@imm.ac.cn
authors.forename: Xianghua
authors.initials: X
authors.lastname: Xu
authors.forename: Yaping
authors.initials: Y
authors.lastname: Pan
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Wang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002545:Brain Ischemia / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002452:Cell Count / Q000379:methods; D002525:Cerebellar Cortex / Q000378:metabolism* / Q000473:pathology; D002908:Chronic Disease; D004195:Disease Models, Animal; D015870:Gene Expression; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D015640:Ion Channel Gating / Q000502:physiology; D008055:Lipids; D008297:Male; D015221:Potassium Channels / Q000096:biosynthesis* / Q000235:genetics; D024683:Potassium Channels, Tandem Pore Domain; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013997:Time Factors
chemical_list: C503444:Kcnk10 protein, rat; C503434:Kcnk4 protein, rat; D008055:Lipids; D015221:Potassium Channels; D024683:Potassium Channels, Tandem Pore Domain; D012333:RNA, Messenger