title: Tanshinone IIA from Salvia miltiorrhiza inhibits inducible nitric oxide synthase expression and production of TNF-alpha, IL-1beta and IL-6 in activated RAW 264.7 cells.
abstract: The inhibitory effects of tanshinone IIA, a diterpene isolated from Salvia miltiorrhiza root, on the production of nitric oxide (NO), interleukin-1beta (IL-1beta), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha), and the expression of inducible nitric oxide synthase (iNOS) were investigated in activated RAW 264.7 cells. This compound markedly inhibited the production of NO, IL-1beta and TNF-alpha, and suppressed the expression of iNOS in a dose-dependent manner. These results suggest that the traditional use of S. miltiorrhiza as an anti-inflammatory herbal medicine may be explained, in part, by the inhibition of NO, IL-1beta, IL-6 and TNF-alpha production, and expression of iNOS.
authors.affiliation: Department of Oral Microbiology, School of Dentistry, Wonkwang University, Iksan, Chonbuk, South Korea.
authors.forename: Seon-Il
authors.initials: SI
authors.lastname: Jang
authors.forename: Seung-Il
authors.initials: SI
authors.lastname: Jeong
authors.forename: Kang-Ju
authors.initials: KJ
authors.lastname: Kim
authors.forename: Hyung-Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Hyeon-Hee
authors.initials: HH
authors.lastname: Yu
authors.forename: Raekil
authors.initials: R
authors.lastname: Park
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Yong-Ouk
authors.initials: YO
authors.lastname: You
mesh_terms: D045784:Abietanes; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007375:Interleukin-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000187:drug effects; D052247:Nitric Oxide Synthase Type II; D010616:Phenanthrenes / Q000737:chemistry / Q000494:pharmacology*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots; D027561:Salvia miltiorrhiza; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000378:metabolism
chemical_list: D045784:Abietanes; D000894:Anti-Inflammatory Agents, Non-Steroidal; D007375:Interleukin-1; D015850:Interleukin-6; D010616:Phenanthrenes; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; C021751:tanshinone; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse