title: Proteomic analysis of lung biopsies: Differential protein expression profile between peritumoral and tumoral tissue.
abstract: The cellular proteome shows a dynamic profile and is subjected to changes in response to various stimuli and disease progression. Lung cancer remains the leading cause of cancer death in industrialized countries. In an attempt to find new disease markers, patients suffering from lung carcinoma have been selected to achieve differential protein expression patterns between normal and neoplasic tissue. After two-dimensional electrophoresis, the spots of interest were digested and identified by matrix-assisted laser desorption/ionization (MALDI) peptide mass fingerprinting. This information will provide a more comprehensive understanding of the disease progression and might constitute a method to complement histopathological diagnosis.
authors.affiliation: Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain. palfonso@cnio.es
authors.forename: Patricia
authors.initials: P
authors.lastname: Alfonso
authors.forename: Myriam
authors.initials: M
authors.lastname: Catalá
authors.forename: María Luisa
authors.initials: ML
authors.lastname: Rico-Morales
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Durante-Rodríguez
authors.forename: Ernesto
authors.initials: E
authors.lastname: Moro-Rodríguez
authors.forename: Héctor
authors.initials: H
authors.lastname: Fernández-García
authors.forename: José M
authors.initials: JM
authors.lastname: Escribano
authors.forename: Emilio
authors.initials: E
authors.lastname: Alvarez-Fernández
authors.forename: Eduardo
authors.initials: E
authors.lastname: García-Poblete
mesh_terms: D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D010449:Peptide Mapping / Q000379:methods*; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020543:Proteome