Effect of astrocytic energy metabolism depressant on 14C-acetate uptake in intact rat brain.
Fluorocitrate, a selective astrocytic toxin, was microinjected into the right striatum of rat brain, and the regional distribution of 14C-acetate was measured using autoradiography. A significant reduction (more than 80%) in 14C-acetate uptake over a 5-minute period was observed in the right striatum, compared with that in the left striatum (saline infused), 4 hours after fluorocitrate (1 nmol/microL) infusion. This effect was transient, and 14C-acetate uptake had almost returned to normal at 24 hours after the fluorocitrate infusion. In contrast, the regional blood flow in the striatum, as determined using 14C-iodoamphetamine, was significantly increased by the fluorocitrate infusion. The present observations indicate that 14C-acetate uptake might be a useful characteristic for examining astrocytic energy metabolism in the intact brain.
Hosoi
Rie
R
School of Allied Health Sciences, Faculty of Medicine, Osaka University, Suita, Osaka, Japan.
Okada
Maki
M
Hatazawa
Jun
J
Gee
Antony
A
Inoue
Osamu
O
D000085:Acetates / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001345:Autoradiography; D001921:Brain / Q000166:cytology / Q000378:metabolism; D002250:Carbon Radioisotopes / Q000378:metabolism; D002951:Citrates / Q000378:metabolism; D004734:Energy Metabolism; D008297:Male; D008845:Microinjections; D051381:Rats; D017208:Rats, Wistar
D000085:Acetates; D002250:Carbon Radioisotopes; D002951:Citrates; C007744:fluorocitrate