Protein stability in nanocages: a novel approach for influencing protein stability by molecular confinement.
Confinement of a protein in a small inert space and microviscosity are known to increase its thermodynamic stability in a way similar to the mechanisms that stabilize protein fold in the cell. Here, to examine the influence of confinement on protein stability we choose four test cases of single domain proteins characterized by a wide range of melting temperatures, from approximately 73 degrees C of titin I27 to approximately 36 degrees C of yeast frataxin. All proteins are stabilized when confined in the gel, the most dramatic stabilization being that of yeast frataxin, whose melting temperature increased by almost 5 degrees C in the gel. In addition to being simple to use, this approach allows us to change the viscosity of the solvent without changing its composition or altering the structure of the proteins. The dimensions of the pores of the gels fall in the nanometer range, hence they are similar to those of the chaperone cavity. This method could therefore be used as a novel and powerful approach for protein folding studies.
Bolis
Dimos
D
Laboratory of Biological Chemistry, Medical School, University of Ioannina, Ioannina 45110, Greece.
Politou
Anastasia S
AS
Kelly
Geoff
G
Pastore
Annalisa
A
Temussi
Piero Andrea
PA
D000180:Acrylic Resins / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D064211:Connectin; D029968:Escherichia coli Proteins; D006801:Humans; D033862:Iron-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D009124:Muscle Proteins / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D011494:Protein Kinases / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature; D013816:Thermodynamics; D014783:Viscosity; D000096923:Frataxin
D000180:Acrylic Resins; D001426:Bacterial Proteins; D064211:Connectin; D029968:Escherichia coli Proteins; D033862:Iron-Binding Proteins; D009124:Muscle Proteins; D011494:Protein Kinases; D000096923:Frataxin; C120906:CyaY protein, E coli; C577285:TTN protein, human; C016680:polyacrylamide gels