title: Determination of chlorophyll in plant samples by liquid chromatography using zinc-phthalocyanine as an internal standard.
abstract: Chlorophyll analysis at high precision and accuracy is limited by the lack of suitable, commercially available internal standards for HPLC analysis. Here, the commercially available dye zinc-phthalocyanine is presented as a new internal standard to quantify chlorophylls in vegetable foods and to detect chlorophyll degradation products. The technique was applied to chlorophyll analysis of a selection of vegetable foods. Pigments were extracted with N,N-dimethylformamide from the vegetables and purified by solid phase extraction. Chlorophyll a, a', b, b', corresponding pheophytins, and zinc-phthalocyanine were separated by HPLC using a C18 reverse-phase column and fluorescence detection.
authors.affiliation: Swiss Federal Institute of Technology (ETH) Zurich, Laboratory of Human Nutrition, Seestrasse 72, 8803 Rüschlikon, Switzerland.
authors.forename: Torsten
authors.initials: T
authors.lastname: Bohn
authors.forename: Thomas
authors.initials: T
authors.lastname: Walczyk
mesh_terms: D002734:Chlorophyll / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007211:Indoles / Q000032:analysis*; D054833:Isoindoles; D009942:Organometallic Compounds / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D017967:Zinc Compounds
chemical_list: D007211:Indoles; D054833:Isoindoles; D009942:Organometallic Compounds; D017967:Zinc Compounds; D002734:Chlorophyll; C052159:Zn(II)-phthalocyanine