title: Comparison between immunomagnetic separation, coupled with immunofluorescence, and the techniques of Faust et al. and of Lutz for the diagnosis of Giardia lamblia cysts in human feces.
abstract: In the present study, the performance of Immunomagnetic Separation technique, coupled with Immunofluorescence (IMS-IFA), was compared with the FAUST et al. and Lutz parasitological techniques for the detection of Giardia lamblia cysts in human feces. One hundred and twenty-seven samples were evaluated by the three techniques at the same time showing a rate of cyst detection of 27.5% by IMS-IFA and 15.7% by both Faust et al. and Lutz techniques. Data analysis showed a higher sensitivity of IMS-IFA for the detection of G. lamblia cysts in comparison with the techniques of FAUST et al. and Lutz. The use of this methodology as a routine procedure enables the processing of many samples simultaneously, in order to increase recovery rate of G. lamblia cysts and reduce the time of sample storage.
authors.affiliation: Universidade Federal de Santa Catarina, Brasil. ccblcrb@ccb.ufsc.br
authors.forename: Doris Sobral Marques
authors.initials: DS
authors.lastname: Souza
authors.forename: Juliana Teixeira
authors.initials: JT
authors.lastname: Barreiros
authors.forename: Karina Maria
authors.initials: KM
authors.lastname: Papp
authors.forename: Mário
authors.initials: M
authors.lastname: Steindel
authors.forename: Cláudia Maria Oliveira
authors.initials: CM
authors.lastname: Simões
authors.forename: Célia Regina Monte
authors.initials: CR
authors.lastname: Barardi
mesh_terms: D000818:Animals; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000469:parasitology; D005455:Fluorescent Antibody Technique / Q000379:methods; D016829:Giardia lamblia / Q000276:immunology* / Q000302:isolation & purification; D005873:Giardiasis / Q000175:diagnosis*; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D010274:Parasitology / Q000379:methods; D012680:Sensitivity and Specificity