title: Flavonoids and a sesquiterpene lactone from Tanacetum microphyllum inhibit anti-inflammatory mediators in LPS-stimulated mouse peritoneal macrophages.
abstract: In our ongoing research into anti-inflammatory compounds from Tanacetum microphyllum, four naturally occurring flavonoids (santin, ermanin, centaureidin and 5,3'-dihydroxy-4'-methoxy-7-methoxycarbonylflavonol) and one sesquiterpene lactone (hydroxyachillin) isolated from this plant, were evaluated as potential inhibitors of some macrophage functions involved in the inflammatory process. These five compounds significantly inhibited lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin E (2) (PGE (2)) in a concentration-dependent manner. In the tumour necrosis factor-alpha (TNF-alpha) assay, only centaureidin and hydroxyachillin significantly inhibited the accumulation of TNF-alpha. These results indicate that these compounds may contribute to the anti-inflammatory properties of T. microphyllum.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University Complutense, Madrid, Spain. mjabad@farm.ucm.es
authors.forename: María José
authors.initials: MJ
authors.lastname: Abad
authors.forename: Paulina
authors.initials: P
authors.lastname: Bermejo
authors.forename: María
authors.initials: M
authors.lastname: Alvarez
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Guerra
authors.forename: Ana María
authors.initials: AM
authors.lastname: Silván
authors.forename: Angel Maria
authors.initials: AM
authors.lastname: Villar
mesh_terms: D000818:Animals; D015232:Dinoprostone / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D007783:Lactones / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D008070:Lipopolysaccharides; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D008517:Phytotherapy; D035261:Plant Components, Aerial; D012717:Sesquiterpenes / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D031203:Tanacetum; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000378:metabolism
chemical_list: D005419:Flavonoids; D007783:Lactones; D008070:Lipopolysaccharides; D012717:Sesquiterpenes; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D015232:Dinoprostone