Role of the PLA2-independent peroxiredoxin VI activity in the survival of immortalized fibroblasts exposed to cytotoxic oxidative stress.
Peroxiredoxin VI (PrxVI) is a bifunctional enzyme with non-selenium glutathione peroxidase and Ca2+-independent acidic phospholipase A2 activities. We demonstrate that transfection-mediated PrxVI overexpression protects immortalized human WI-38 and murine NIH3T3 fibroblasts against cytotoxic doses of tert-butylhydroperoxide and H2O2. Mutants for either glutathione peroxidase or phospholipase A2 activity show that glutathione peroxidase but not phospholipase A2 activity is required to promote cell survival after stress. Also, ectopic PrxVI overexpression does not protect telomerase-stabilized WI-38 fibroblasts against stress-induced premature senescence.
Salmon
Michel
M
Research Unit of Cellular Biology, University of Namur (FUNDP), rue de Bruxelles 61, B-5000 Namur, Belgium.
Dedessus Le Moutier
Jérémie
J
Wenders
Frédéric
F
Chiarizia
Sophie
S
Eliaers
François
F
Remacle
José
J
Royer
Véronique
V
Pascal
Thierry
T
Toussaint
Olivier
O
D016475:3T3 Cells; D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D016922:Cellular Senescence / Q000187:drug effects / Q000502:physiology*; D005347:Fibroblasts / Q000166:cytology* / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D051379:Mice; D018384:Oxidative Stress / Q000502:physiology*; D010544:Peroxidases / Q000235:genetics* / Q000378:metabolism; D054465:Peroxiredoxin VI; D054464:Peroxiredoxins; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection; D020122:tert-Butylhydroperoxide / Q000633:toxicity*
D011994:Recombinant Proteins; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D010544:Peroxidases; D054465:Peroxiredoxin VI; D054464:Peroxiredoxins; D010741:Phospholipases A; D054467:Phospholipases A2