title: A single WAP domain-containing protein from Litopenaeus vannamei hemocytes.
abstract: A cDNA clone coding for a single WAP domain (SWD) protein was isolated from a hemocyte cDNA library of Litopenaeus vannamei. The full-length cDNA sequence is 0.4kb long and encodes a 93-amino acid protein. Using this sequence as a probe a similar clone coding for a 92-amino acids protein was found in a cDNA library from Penaeus monodon hemocytes. The mRNA size was confirmed by Northern blot as well as that gene is expressed in hemocytes, but not in hepatopancreas. mRNA levels of the shrimp SWD protein were modified after injection of Vibrio alginolyticus, indicating the probable role of this protein in the immune response. Although amino acid sequence seems to be similar to those of other WAP domain-containing proteins, shrimp SWD protein does not have any other functional domain, similar to a mouse single WAP motif (SWAM) protein reported in mouse; however, the phylogenetic analysis shows that shrimp SWD is more related to other WAP proteins than to mouse SWAM.
authors.affiliation: CIBNOR, PO Box 128, 23000, La Paz, BCS, Mexico.
authors.forename: Florinda
authors.initials: F
authors.lastname: Jiménez-Vega
authors.forename: Gloria
authors.initials: G
authors.lastname: Yepiz-Plascencia
authors.forename: Kenneth
authors.initials: K
authors.lastname: Söderhäll
authors.forename: Francisco
authors.initials: F
authors.lastname: Vargas-Albores
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000276:immunology; D015723:Gene Library; D006434:Hemocytes / Q000378:metabolism*; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D044149:Vibrio alginolyticus / Q000276:immunology
chemical_list: D011506:Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins