title: Biological rhythmicity in expressed proteins of the marine dinoflagellate Lingulodinium polyedrum demonstrated by chronological proteomics.
abstract: The dinoflagellate Lingulodinium polyedrum shows circadian expression of proteins. Analysis of the expression profile of ca. 900 proteins (pI range 5-8; MW 22,000-120,000) by chronological proteomics in 12:12h light-dark ('day-night') cycles revealed 28 with quantitative circadian changes. The patterns were classified into three types: Phase 1 ('evening'), Phase 2 ('night'), and Phase 3 ('midnight'). Peptide mass fingerprinting identified luciferin-binding protein in Phase 2 and two components of the tricarboxylic acid cycle in Phases 1 and 2. Thus, rhythmicity appears to be related to physiological states and to control the metabolic pathway in L. polyedrum.
authors.affiliation: Light and Control Research Area, PRESTO, Japan Science and Technology Corporation, 1-3-1 Kashiwadaiminami, Chitose, Hokkaido, Japan. h-akimoto@aist.go.jp
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Akimoto
authors.forename: Chun
authors.initials: C
authors.lastname: Wu
authors.forename: Tomoya
authors.initials: T
authors.lastname: Kinumi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ohmiya
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D002940:Circadian Rhythm; D002952:Citric Acid Cycle; D004141:Dinoflagellida / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D049454:Firefly Luciferin / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements; D013058:Mass Spectrometry; D008954:Models, Biological; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry; D015800:Protozoan Proteins; D013997:Time Factors
chemical_list: D002135:Calcium-Binding Proteins; D010455:Peptides; D015800:Protozoan Proteins; C065397:luciferin-binding protein, dinoflagellate; D049454:Firefly Luciferin