title: [Chemical and chemico-enzymatic synthesis of alpha-thiotriphosphate nucleosides].
abstract: New methods of chemical and chemoenzymatic synthesis of nucleoside 5'-thiophosphates and 5'-alpha-thiotriphosphates are developed. The 5'-alpha-thiotriphosphates are used as substrates both in template-dependent enzymatic PCR synthesis and in a T7-RNA transcription polymerase system. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2003, vol. 29, no. 6; see also http://www.maik.ru.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP Moscow, 117997 Russia. kid@ibch.ru
authors.forename: K V
authors.initials: KV
authors.lastname: Antonov
authors.forename: R S
authors.initials: RS
authors.lastname: Esipov
authors.forename: A I
authors.initials: AI
authors.lastname: Gurevich
authors.forename: D V
authors.initials: DV
authors.lastname: Chuvikovskiĭ
authors.forename: G V
authors.initials: GV
authors.lastname: Mikulinskaia
authors.forename: S A
authors.initials: SA
authors.lastname: Feofanov
authors.forename: A I
authors.initials: AI
authors.lastname: Miroshnikov
mesh_terms: D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D019906:Nuclear Magnetic Resonance, Biomolecular; D009705:Nucleosides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000737:chemistry*; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D014764:Viral Proteins
chemical_list: D009705:Nucleosides; D009943:Organophosphorus Compounds; D013438:Sulfhydryl Compounds; D014764:Viral Proteins; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases