title: Epistatic effects of genes encoding immunoglobulin GM allotypes and interleukin-6 on the production of autoantibodies to 60- and 65-kDa heat-shock proteins.
abstract: Immunoglobulin GM and KM genes have been associated with antibody responses to a variety of antigens. A promoter-region polymorphism of interleukin-6 (IL-6) gene (-174 G/C) has been shown to be associated with antibody responses to heat-shock proteins (hsp) 60 and hsp65. To examine the possible epistatic effects of these unlinked genetic systems on the autoimmune responses to hsp60 and hsp65, 176 healthy Caucasian subjects from Finland were genotyped for several allelic determinants of GM, KM, and IL-6 genes by PCR-RFLP methods. IgG antibodies to hsp60 and hsp65 were measured by an ELISA. Significant interactive effects of GM f,z and IL-6-174 genotypes were noted for both anti-hsp60 (P=0.002) and anti-hsp65 (P=0.038) antibody levels. Since these autoantibodies have been implicated in susceptibility to coronary heart disease and carotid atherosclerosis, the associations reported here might be relevant to the etiology of these diseases.
authors.affiliation: Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 29425, USA. pandeyj@musc.edu
authors.forename: J P
authors.initials: JP
authors.lastname: Pandey
authors.forename: Z
authors.initials: Z
authors.lastname: Prohászka
authors.forename: A
authors.initials: A
authors.lastname: Veres
authors.forename: G
authors.initials: G
authors.lastname: Füst
authors.forename: M
authors.initials: M
authors.lastname: Hurme
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000097:blood / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D002340:Carotid Artery Diseases / Q000209:etiology / Q000235:genetics / Q000276:immunology; D018834:Chaperonin 60 / Q000276:immunology*; D018833:Chaperonins / Q000276:immunology*; D003327:Coronary Disease / Q000209:etiology / Q000235:genetics / Q000276:immunology; D004843:Epistasis, Genetic; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D020022:Genetic Predisposition to Disease; D006801:Humans; D007129:Immunoglobulin Gm Allotypes / Q000097:blood / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D001323:Autoantibodies; D001426:Bacterial Proteins; D018834:Chaperonin 60; D000939:Epitopes; D007129:Immunoglobulin Gm Allotypes; D015850:Interleukin-6; D011994:Recombinant Proteins; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins