title: Association of IL-1beta, IL-1ra, and TNF-alpha gene polymorphisms in childhood nephrotic syndrome.
abstract: We investigated the association between IL-1beta, IL-1ra, and TNF-alpha gene polymorphisms and childhood nephrotic syndrome (NS). We analyzed the genetic polymorphism of IL-1beta, IL-1ra, and TNF-alpha genes in 152 patients with childhood NS and 292 healthy adult controls. The C to T exchange at position -511 of IL-1beta and the G to A at -308 of the TNF-alpha gene were genotyped. Five alleles of the IL-1ra gene were identified and designated as IL1RN*1, IL1RN*2, IL1RN*3, IL1RN*4, and IL1RN*5, according to the variable number of tandem repeats in intron 2. The allele frequencies of IL-1beta1 (-511C), IL-1beta2 (-511T), TNF1 (-308G), and TNF2 (-308A) were 53.0, 47.0, 92.1, and 7.9%, respectively, in the childhood NS group. This was not significantly different from normal controls. In the childhood NS group, the allele frequencies of IL1RN*1, IL1RN*2, IL1RN*3, IL1RN*4, and IL1RN*5 were 90.8, 7.6, 1.6, 0, and 0% [IL1RN*1 odds ratio (OR)=0.296, P=0.0001, IL1RN*2 OR=3.902, P=0.0002]. A high allele frequency of IL1RN*2 and a lower allele frequency of IL1RN*1 were found in childhood NS, although there was no association with IL-1beta and TNF-alpha. A high allele frequency of the IL1RN*2 allele may affect disease susceptibility in childhood NS.
authors.affiliation: East West Kidney Disease Research Institute, College of Medicine, Kyung Hee University Hospital, Seoul, Korea.
authors.forename: Sung-Do
authors.initials: SD
authors.lastname: Kim
authors.forename: Jae-Man
authors.initials: JM
authors.lastname: Park
authors.forename: Il-Soo
authors.initials: IS
authors.lastname: Kim
authors.forename: Kang-Duk
authors.initials: KD
authors.lastname: Choi
authors.forename: Byung-Cheol
authors.initials: BC
authors.lastname: Lee
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Seung-Jae
authors.initials: SJ
authors.lastname: Hong
authors.forename: Sheng-Yu
authors.initials: SY
authors.lastname: Jin
authors.forename: Hee-Jae
authors.initials: HJ
authors.lastname: Lee
authors.forename: Mee-Suk
authors.initials: MS
authors.lastname: Hong
authors.forename: Joo-Ho
authors.initials: JH
authors.lastname: Chung
authors.forename: Tae-Won
authors.initials: TW
authors.lastname: Lee
authors.forename: Chun-Gyoo
authors.initials: CG
authors.lastname: Ihm
authors.forename: Byoung-Soo
authors.initials: BS
authors.lastname: Cho
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009404:Nephrotic Syndrome / Q000235:genetics*; D011110:Polymorphism, Genetic; D012795:Sialoglycoproteins / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D012795:Sialoglycoproteins; D014409:Tumor Necrosis Factor-alpha