title: Proteomic evaluation of core biopsy specimens from breast lesions.
abstract: Analysis of tumour samples by a proteomic technology, which combines two-dimensional gel electrophoresis and mass spectrometry analysis, is a promising approach for molecular characterization of cancer. Proteomic analysis of neoplasms is usually performed on surgical material. The possibility to perform proteomic analysis on pre-operative samples might be useful for diagnostic purposes or for determination of tumour sensitivity to therapy. In this study, we report how tissues from core biopsy of breast lesions can be routinely used to obtain accurate protein expression profiles by proteomic analysis. Protein profiles from fibroadenomas were compared to those from ductal infiltrating carcinomas. By using mass spectrometry, identification of proteins overexpressed in carcinomas with respect to fibroadenomas was obtained. Thus, our study provides a methodology to perform proteomic analysis on pre-operative samples of breast lesions.
authors.affiliation: Dipartimento di Scienze e Tecnologie Biomediche, Centro M.A.T.I., Università di Udine, Piazzale Kolbe 1, Udine 33100, Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Bisca
authors.forename: Chiara
authors.initials: C
authors.lastname: D'Ambrosio
authors.forename: Andrea
authors.initials: A
authors.lastname: Scaloni
authors.forename: Fabio
authors.initials: F
authors.lastname: Puglisi
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Aprile
authors.forename: Andrea
authors.initials: A
authors.lastname: Piga
authors.forename: Chiara
authors.initials: C
authors.lastname: Zuiani
authors.forename: Massimo
authors.initials: M
authors.lastname: Bazzocchi
authors.forename: Carla
authors.initials: C
authors.lastname: Di Loreto
authors.forename: Igor
authors.initials: I
authors.lastname: Paron
authors.forename: Gianluca
authors.initials: G
authors.lastname: Tell
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Damante
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D001706:Biopsy; D001943:Breast Neoplasms / Q000737:chemistry* / Q000175:diagnosis; D018270:Carcinoma, Ductal, Breast / Q000737:chemistry* / Q000175:diagnosis; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D018226:Fibroadenoma / Q000737:chemistry* / Q000175:diagnosis; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis*; D010446:Peptide Fragments / Q000737:chemistry; D010449:Peptide Mapping; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D010446:Peptide Fragments