title: LNCIB human full-length cDNAs collection: towards a better comprehension of the human transcriptome.
abstract: LNCIB has been producing a variety of human full-length-enriched, normalized and subtracted cDNA libraries from various cell lines and tissues in different developmental stages by using the CAP-Trapper method. By sequencing 23000 clones of these libraries we identified a pool of about 5800 good quality unique cDNAs. After BLAST analysis on Human RefSeq/Unigene databases, 1717 of these sequences remained with no or poor annotation. We show that cross-species comparative BLAST resulted as a valid tool for the annotation of orthologous genes.
authors.affiliation: Laboratorio Nazionale CIB, Functional Genomics Group, AREA Science Park, Padriciano 99, Trieste 34012, Italy.
authors.forename: Emiliano
authors.initials: E
authors.lastname: Dalla
authors.forename: Roberto
authors.initials: R
authors.lastname: Verardo
authors.forename: Dejan
authors.initials: D
authors.lastname: Lazarević
authors.forename: Luigi
authors.initials: L
authors.lastname: Marchionni
authors.forename: James F
authors.initials: JF
authors.lastname: Reid
authors.forename: Nabil
authors.initials: N
authors.lastname: Bahar
authors.forename: Enio
authors.initials: E
authors.lastname: Klarić
authors.forename: Giacomo
authors.initials: G
authors.lastname: Marcuzzi
authors.forename: Riccardo
authors.initials: R
authors.lastname: Marzio
authors.forename: Anna
authors.initials: A
authors.lastname: Belgrano
authors.forename: Danilo
authors.initials: D
authors.lastname: Licastro
authors.forename: Claudio
authors.initials: C
authors.lastname: Schneider
mesh_terms: D016208:Databases, Factual; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D014158:Transcription, Genetic