Crystallization and preliminary X-ray analysis of xylanase B from Clostridium stercorarium.
Recombinant mature xylanase B from Clostridium stercorarium has been prepared and crystallized by the sitting-drop vapour-diffusion method using 4 mg ml(-1) purified enzyme, 10.3%(w/v) polyethylene glycol 1500, 8.6%(v/v) glycerol and 0.34 M non-detergent sulfobetaine 195. A suitable crystal grew after incubation for ten weeks at 293 K. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 64.76, b = 96.60, c = 138.44 A. X-ray diffraction data were collected to 1.80 A resolution.
Nishimoto
Mamoru
M
National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Fushinobu
Shinya
S
Miyanaga
Akimasa
A
Wakagi
Takayoshi
T
Shoun
Hirofumi
H
Sakka
Kazuo
K
Ohmiya
Kunio
K
Nirasawa
Satoru
S
Kitaoka
Motomitsu
M
Hayashi
Kiyoshi
K
D003013:Clostridium / Q000201:enzymology*; D018360:Crystallography, X-Ray; D003902:Detergents / Q000494:pharmacology; D004058:Diffusion; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry*; D004926:Escherichia coli / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D013696:Temperature
D003902:Detergents; D011994:Recombinant Proteins; D011092:Polyethylene Glycols; D043364:Endo-1,4-beta Xylanases