title: Expression of the chondromodulin-I gene in chondrosarcomas.
abstract: We investigated the expression of the Chondromodulin-I (ChM-I) gene, a putative tumor suppressor gene in cartilaginous tumors, by quantitative RT-PCR in 15 chondrosarcomas (CSs). Eight CSs expressed the ChM-I gene at the level higher than those in articular cartilage (positive cases), whereas the expression of the ChM-I gene in the remaining seven CSs was lower than those in articular cartilage (negative cases). All of five peripheral CS were positive, and the ChM-I positive tumors shared expression profiles of cartilage-related genes with articular cartilage cells. On the other hand, all of four central CSs without extramedullary lesions were negative, and the ChM-I negative tumors expressed the parathyroid hormone-related peptide gene at the lower level and the COL10A1 genes at the higher level than articular cartilage cells. Neither the histological grade nor the rate of recurrence showed clear association with the level of ChM-I gene expression. These results suggested that the expression of ChM-I gene in CS has no direct role in tumorigenesis but rather reflects the site of tumor development and therefore precursor of tumor cells.
authors.affiliation: Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Aoyama
authors.forename: Takeshi
authors.initials: T
authors.lastname: Okamoto
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nagayama
authors.forename: Koichi
authors.initials: K
authors.lastname: Nishijo
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Ishibe
authors.forename: Ko
authors.initials: K
authors.lastname: Yasura
authors.forename: Tadao
authors.initials: T
authors.lastname: Tsuboyama
authors.forename: Tomitaka
authors.initials: T
authors.lastname: Nakayama
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Nakashima
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakamura
authors.forename: Junya
authors.initials: J
authors.lastname: Toguchida
mesh_terms: D053674:Aggrecans; D020533:Angiogenesis Inhibitors / Q000235:genetics* / Q000378:metabolism; D001859:Bone Neoplasms / Q000235:genetics*; D002356:Cartilage / Q000378:metabolism; D002813:Chondrosarcoma / Q000235:genetics*; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D000090602:Collagen Type I, alpha 1 Chain; D024081:Collagen Type IX / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D044162:Parathyroid Hormone-Related Protein / Q000235:genetics / Q000378:metabolism; D011509:Proteoglycans / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D053674:Aggrecans; D020533:Angiogenesis Inhibitors; C439970:COL9A1 protein, human; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D024081:Collagen Type IX; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D044162:Parathyroid Hormone-Related Protein; D011509:Proteoglycans; D012333:RNA, Messenger; C068675:CNMD protein, human