Microcontact printing of proteins on oxygen plasma-activated poly(methyl methacrylate).
This paper describes a method for microcontact printing protein solutions onto polymer substrates temporarily activated by oxygen plasma. Following plasma treatment, poly(dimethyl siloxane) (PDMS) stamps were coated with an aqueous laminin solution then placed in direct contact with plasma-treated poly(methyl methacrylate) (PMMA) substrates. This process resulted in well defined laminin stripes on the PMMA surface when printing was performed within 45min of the plasma treatment. Axonal outgrowth from embryonic chick dorsal root ganglia (DRG) was largely confined to the stamped pattern, while over 90% of primary rat Schwann cells adhered to the protein stamped areas on the PMMA substrates. Oxygen-plasma treatment of the PMMA surface was necessary to deposit proteins that direct axonal outgrowth from chick DRG and Schwann cell adherence.
Schmalenberg
K E
KE
Department of Chemistry and Chemical Biology, 610 Taylor Road, Rutgers University, Piscataway, NJ 08854, USA.
Buettner
H M
HM
Uhrich
K E
KE
D000327:Adsorption; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000502:physiology; D016764:Cell Polarity / Q000502:physiology; D002478:Cells, Cultured; D002642:Chick Embryo; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000378:metabolism; D006358:Hot Temperature; D007797:Laminin / Q000737:chemistry / Q000378:metabolism*; D008422:Materials Testing; D008832:Microchemistry / Q000379:methods*; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D010100:Oxygen / Q000737:chemistry; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D011327:Printing / Q000379:methods; D051381:Rats; D012583:Schwann Cells / Q000166:cytology* / Q000502:physiology; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D007797:Laminin; D019904:Polymethyl Methacrylate; D010100:Oxygen