title: Regulation of interleukin-6 fetoplacental levels could be involved in the protective effect of low-molecular weight heparin treatment on murine spontaneous abortion.
abstract: OBJECTIVE
  CBA/J x DBA/2 abortion rate could be the consequence of a deficient local production of T helper (Th2) cytokines, which cause fetal wastage via fgl2 prothrombinase. Heparin reduces significantly the abortion rate in mice and recurrent spontaneous abortion (RSA) patients. We proposed to determine the effect of enoxaparin on the levels of local interleukin (IL)-6 during murine pregnancy.
  
  
  METHODS
  Recombinant human IL-6 (rhIL-6) or enoxaparin were inoculated in CBA/J x DBA/2 pregnant mice on days 6.5-12.5. IL-6 levels in sera as well as in culture supernatants of day 9.5 fetoplacental units of CBA/J x BALB/c control mice or CBA/J x DBA/2 abortion combination were determined by enzyme-linked immunosorbent assay (ELISA) test.
  
  
  RESULTS
  CBA/J x DBA/2 fetoplacental units secreted significantly lower levels of IL-6 with regard to CBA/J x BALB/c normal units. rhIL-6h and enoxaparin treatments decreased the resorption rate and regulated IL-6 fetoplacental levels.
  
  
  CONCLUSIONS
  This study suggests that regulation of IL-6 fetoplacental levels could be involved in heparin-mediated anticoagulation protection against abortion.
authors.affiliation: Institute of Immunology, IDEHU, Univerity of Buenos Aires, Buenos Aires, Argentina. gabgutie@ffyb.uba.ar
authors.forename: G
authors.initials: G
authors.lastname: Gutiérrez
authors.forename: A
authors.initials: A
authors.lastname: Sarto
authors.forename: L
authors.initials: L
authors.lastname: Berod
authors.forename: A
authors.initials: A
authors.lastname: Canellada
authors.forename: T
authors.initials: T
authors.lastname: Gentile
authors.forename: S
authors.initials: S
authors.lastname: Pasqualini
authors.forename: R A
authors.initials: RA
authors.lastname: Margni
mesh_terms: D000022:Abortion, Spontaneous / Q000188:drug therapy* / Q000378:metabolism; D000818:Animals; D046508:Culture Techniques; D005260:Female; D005327:Fetal Resorption / Q000188:drug therapy; D006495:Heparin, Low-Molecular-Weight / Q000627:therapeutic use*; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000627:therapeutic use*; D051379:Mice; D010920:Placenta / Q000166:cytology / Q000378:metabolism*; D011247:Pregnancy
chemical_list: D006495:Heparin, Low-Molecular-Weight; D015850:Interleukin-6