title: Long-term morphometric and immunohistochemical findings in human free microvascular muscle flaps.
abstract: Reinnervation, muscle regeneration, density of microvessels, and muscle-type specific atrophy were studied 3-4 years after surgery in surgically nonreinnervated free microvascular muscle flaps to 13 patients transplanted to the upper or lower extremities. Routine histology and immunohistochemistry for PGP 9.5 and S-100 (neuronal markers), Ki-67 (cell proliferation), myosin (muscle fiber types), and CD-31 (endothelium) were carried out, and results were analyzed morphometrically. Three to 4 years after surgery, severe atrophy of predominantly slow-type fibers was seen in 9 cases. In 4 cases, muscle-fiber diameter and fiber-type distribution were close to normal. Long intraoperative muscle ischemia and postoperative immobilization were associated with poor muscle bulk in flaps. The density of microvessels in flaps did not differ from control muscles. PGP 9.5 and S-100 immunopositive nerve fibers were detected in 7 patients. Reinnervation was associated with good muscle bulk. In 4 patients, activation of satellite cells was evident. The results suggest that in some cases, spontaneous reinnervation may occur in free muscle flaps, and that several years after microvascular free flap transfer, the muscle still attempts to regenerate.
authors.affiliation: Department of Plastic Surgery, Töölö Hospital, Helsinki University Hospital, Helsinki, Finland. susanna.kauhanen@hus.fi
authors.forename: M Susanna C
authors.initials: MS
authors.lastname: Kauhanen
authors.forename: Fulvio
authors.initials: F
authors.lastname: Lorenzetti
authors.forename: Ilmo V V
authors.initials: IV
authors.lastname: Leivo
authors.forename: Erkki
authors.initials: E
authors.lastname: Tukiainen
authors.forename: Sirpa L
authors.initials: SL
authors.lastname: Asko-Seljavaara
mesh_terms: D000328:Adult; D002455:Cell Division; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000502:physiology; D018482:Muscle, Skeletal / Q000502:physiology / Q000601:surgery; D009133:Muscular Atrophy / Q000473:pathology; D012038:Regeneration; D013524:Surgical Flaps / Q000098:blood supply / Q000294:innervation / Q000502:physiology; D013997:Time Factors