Neural cell fate analysis in zebrafish using olig2 BAC transgenics.
We describe here simple methods for producing transgenic zebrafish reporter lines using BAC clones. The use of BAC clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Combined with recent advances in live embryo image analysis, this strategy has the potential to greatly advance the investigation of neural cell behavior during development.
Shin
Jimann
J
Vanderbilt University, Department of Biological Sciences, Nashville, TN 37232, USA.
Park
Hae-Chul
HC
Topczewska
Jolanta M
JM
Mawdsley
David J
DJ
Appel
Bruce
B
D000818:Animals; D003001:Cloning, Molecular; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D008845:Microinjections; D009046:Motor Neurons / Q000166:cytology / Q000378:metabolism*; D017933:Peripheral Nervous System / Q000166:cytology / Q000378:metabolism*; D011689:Purkinje Cells / Q000166:cytology / Q000378:metabolism*; D015027:Zebrafish / Q000033:anatomy & histology / Q000235:genetics* / Q000378:metabolism
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins