Identification and assay of wild-type and modified nucleoside mixtures by turbo ionspray ionization tandem mass spectrometry.
A method is presented which allows the identification and assay of a nucleoside in the presence of other analogues and homologues. The method is based on the conventional multiple reaction monitoring approach performed on the [M + H]+ ions of wild-type and modified nucleosides produced by the turbo ionspray ionization method on a triple-quadrupole mass spectrometer. The accuracy of the quantitative determination relies on the evaluation of a response factor rho, which takes into account the kinetics of dissociation of the parent ions into the protonated [B + 2H]+ nucleobase ions. The evaluation of the absolute concentration of each analyte in the examined mixture does not require any previous chromatographic separation.
Benincasa
Cinzia
C
Dipartimento di Chimica, Università della Calabria, via P. Bucci, cubo 15/C I-87030 Arcavacata di Rende (CS), Italy.
Maiuolo
Loredana
L
Mazzotti
Fabio
F
Perri
Enzo
E
Sindona
Giovanni
G
Urso
Elena
E
D015394:Molecular Structure; D009705:Nucleosides / Q000032:analysis* / Q000737:chemistry; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D009705:Nucleosides