title: Active components from Artemisia iwayomogi displaying ONOO(-) scavenging activity.
abstract: Peroxynitrite (ONOO(-)) is one of cytotoxic species produced by the reaction between superoxide (*O(2) (-)) and nitric oxide (NO). The main aim of this study was to identify ONOO(-) scavenging constituents from herbs. Methanolic extracts derived from one hundred thirty eight herbs were screened out for their ONOO(-) scavenging activities. Twenty herbs showed excellent ONOO(-) scavenging activity in a dose dependent manner. Of them, Artemisia iwayomogi, which showed the highest activity, was fractioned with several solvents. The ONOO(-) scavenging activity of fractions was in order of ethyl acetate (EtOAc) > n-butanol (BuOH) > dichloromethane (CH(2)Cl(2)) > water (H(2)O) fraction. The EtOAc and the n-BuOH fractions were further purified to isolate active principles by column chromatography. Apigenin 7-methylether (genkwanin), apigenin 7,4'-di-O methylether, jaceosidin, and scopoletin from the EtOAc fraction and chlorogenic acid, 2,4-dihydroxy 6-methoxy acetophenone 4-O-beta-D-glucoside, quebrachitol, and scopolin from the n-BuOH fraction were identified. The scavenging activities of chlorogenic acid (IC(50) = 0.52 +/- 0.04 microM), genkwanin (IC(50) = 1.01 +/- 0.10 microM), and scopoletin (IC(50) = 1.03 +/- 0.15 micro M) were higher than or comparable to a well-known ONOO(-) scavenger, penicillamine (IC(50) = 1.76 +/- 0.18 microM), suggesting that those compounds might be developed as effective ONOO(-) scavengers for, prevention of the ONOO(-)-involved diseases.
authors.affiliation: College of Pharmacy, Pusan National University, Pusan, Korea.
authors.forename: Ae Ra
authors.initials: AR
authors.lastname: Kim
authors.forename: Ya Ni
authors.initials: YN
authors.lastname: Zou
authors.forename: Tae Hyun
authors.initials: TH
authors.lastname: Park
authors.forename: Kyung Hee
authors.initials: KH
authors.lastname: Shim
authors.forename: Min Sun
authors.initials: MS
authors.lastname: Kim
authors.forename: Nam Deuk
authors.initials: ND
authors.lastname: Kim
authors.forename: Jong Deog
authors.initials: JD
authors.lastname: Kim
authors.forename: Song Ja
authors.initials: SJ
authors.lastname: Bae
authors.forename: Jae Sue
authors.initials: JS
authors.lastname: Choi
authors.forename: Hae Young
authors.initials: HY
authors.lastname: Chung
mesh_terms: D019306:Artemisia; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D020128:Inhibitory Concentration 50; D030421:Peroxynitrous Acid / Q000737:chemistry*; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D016166:Free Radical Scavengers; D010936:Plant Extracts; D030421:Peroxynitrous Acid