Effect of iron limitation on the growth and cytotoxin production of Salmonella choleraesuis SC-5.
This study investigated the effect of iron limitation on the growth and cytotoxin production of Salmonella choleraesuis and examined the iron-accruing capability of various Salmonella strains. It was found that the growth of S. choleraesuis SC-5 was retarded by the presence of iron-chelating agents, 2,2'-dipyridyl or ethylenediamine di-o-hydroxyphenylactic acid (EDDA). Addition of 2,2'-dipyridyl to Trypticase soy broth (TSB) resulted in a smaller maximum population of S. choleraesuis noted at the stationary phase, while addition of EDDA in TSB only caused an extended period of lag phase. A significant increase in cytotoxin production was found when S. choleraesuis SC-5 was cultured in iron-limited TSB containing 2,2'-dipyridyl. On the other hand, pre-culture in an iron-limited medium increased the growth of S. choleraesuis SC-5 in mouse serum with or without complement inactivated. Testing with a plate assay method revealed that the ability and efficiency of Salmonella to acquire iron under iron-limited condition varied with strains, and the kinds and dosages of iron-containing compounds present.
Ho
Wei-Li
WL
Department of Food Science, Ching Kuo Institute of Management and Health, Keelung, Taiwan.
Yu
Roch-Chui
RC
Chou
Cheng-Chun
CC
D015082:2,2'-Dipyridyl / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry; D003603:Cytotoxins / Q000096:biosynthesis*; D004492:Edetic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D005519:Food Preservation; D007501:Iron / Q000378:metabolism* / Q000494:pharmacology; D007502:Iron Chelating Agents / Q000494:pharmacology*; D012475:Salmonella / Q000254:growth & development* / Q000378:metabolism*; D013045:Species Specificity
D003470:Culture Media; D003603:Cytotoxins; D007502:Iron Chelating Agents; D015082:2,2'-Dipyridyl; C026058:EDDA; D004492:Edetic Acid; D007501:Iron