Platelet dysfunction in cardiopulmonary bypass: an experimental comparative study between a centrifugal and a new pulsatile pump.
The aim of this investigation was to study the effect of a new pulsatile pump for extracorporeal circulation (ECC) on platelet count and platelet function with respect to a Biomedicus centrifugal pump. Thirteen pigs, 8 in the pulsatile group (PG) and 5 in the centrifugal group (CG), underwent a partial extracorporeal circulation lasting 3 h. The animals were sacrificed 3 h post-ECC. The platelet study was both quantitative (platelet count) and qualitative (platelet function analysis) by assessing the closure time (CT) with a PFA-100 system. The decrease in platelet number from basal to 3 h post ECC was only significant in CG (p = 0.009). The platelet function was impaired in both groups, but the value of CT with col/ADP increased significantly only in CG (p < 0.001). The increase of CT with col/EPI was greater in CG (p = 0.07) than in PG (p = 0.2). The results indicated that the new pulsatile pump preserves platelets quantitatively and qualitatively well compared to a Biomedicus pump.
Herreros
J
J
Cardiovascular Surgery Department, Clinica Universitaria, University of Navarra, Pamplona, Spain.
Berjano
E J
EJ
Más
P
P
Padrós
C
C
Sales-Nebot
L
L
Vlaanderen
W
W
Díaz
P
P
Páramo
J A
JA
Rábago
G
G
Mercé
S
S
D000136:Acid-Base Equilibrium / Q000502:physiology; D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D002315:Cardiopulmonary Bypass; D002498:Centrifugation; D005112:Extracorporeal Circulation; D006400:Hematocrit; D006452:Hemoglobinometry; D018056:Hemorheology; D007958:Leukocyte Count; D015539:Platelet Activation / Q000502:physiology*; D010976:Platelet Count; D011673:Pulsatile Flow; D013552:Swine