Inhibitory effect of tellimagrandin I on chemically induced differentiation of human leukemia K562 cells.
Tellimagrandin I is a hydrolysable tannin compound widely present in plants. In this study, the effect of tellimagrandin I on chemically induced erythroid and megakaryocytic differentiation was investigated using K562 cells as differentiation model. It was found that tellimagrandin I not only inhibited the hemoglobin synthesis in butyric acid (BA)- and hemin-induced K562 cells with IC50 of 3 and 40microM, respectively, but also inhibited other erythroid differentiation marker including acetylcholinesterase (AChE) and glycophorin A (GPA) in BA-induced K562 cells. Tellimagrandin I also inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced expression of CD61 protein, a megakaryocytic marker. RT-PCR analysis showed that tellimagrandin I decreased the expression of erythroid genes (gamma-globin and porphobilinogen deaminase (PBGD)) and related transcription factors (GATA-1 and NF-E2) in BA-induced K562 cells, whereas tellimagrandin I induced the overexpresison of GATA-2 transcription factor that played negative regulation on erythroid differentiation. These results indicated that tellimagrandin I had inhibitory effects on erythroid and megakaryocytic differentiation, which suggested that tannins like tellimagrandin I might influence the anti-tumor efficiency of some drugs and the hematopoiesis processes.
Yi
Zongchun
Z
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
Wang
Zhao
Z
Li
Haixia
H
Liu
Mingjie
M
D000110:Acetylcholinesterase / Q000378:metabolism; D001560:Benzidines / Q000737:chemistry; D002454:Cell Differentiation / Q000187:drug effects*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000502:physiology; D015672:Erythroid Precursor Cells / Q000187:drug effects; D050981:Erythroid-Specific DNA-Binding Factors; D005434:Flow Cytometry; D050982:GATA1 Transcription Factor; D050989:GATA2 Transcription Factor; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D005960:Glucosides / Q000494:pharmacology*; D006021:Glycophorins / Q000378:metabolism; D006410:Hematopoiesis / Q000187:drug effects; D006454:Hemoglobins / Q000096:biosynthesis; D006801:Humans; D011163:Hydroxymethylbilane Synthase / Q000378:metabolism; D020014:K562 Cells; D008533:Megakaryocytes / Q000187:drug effects; D050983:NF-E2 Transcription Factor; D050984:NF-E2 Transcription Factor, p45 Subunit; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014157:Transcription Factors / Q000502:physiology
D001560:Benzidines; D002800:Cholinesterase Inhibitors; D017931:DNA Primers; D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; C494688:GATA1 protein, human; D050989:GATA2 Transcription Factor; C494711:GATA2 protein, human; D005960:Glucosides; D006021:Glycophorins; D006454:Hemoglobins; D050983:NF-E2 Transcription Factor; D050984:NF-E2 Transcription Factor, p45 Subunit; C494692:NFE2 protein, human; D012333:RNA, Messenger; D014157:Transcription Factors; C029876:benzidine; D005707:Gallic Acid; C087926:tellimagrandin I; D011163:Hydroxymethylbilane Synthase; D000110:Acetylcholinesterase; D013755:Tetradecanoylphorbol Acetate