Hemoglobin-vesicles suspended in recombinant human serum albumin for resuscitation from hemorrhagic shock in anesthetized rats.
OBJECTIVE
Hemoglobin-vesicle (HbV) has been developed to provide oxygen-carrying ability to plasma expanders. Its ability to restore the systemic condition after hemorrhagic shock was evaluated in anesthetized Wistar rats for 6 hrs after resuscitation. The HbV was suspended in 5 g/dL recombinant human serum albumin (HbV/rHSA) at an Hb concentration of 8.6 g/dL.


METHODS
Prospective, randomized, controlled trial.


METHODS
Department of Surgery, School of Medicine, Keio University.


METHODS
Forty male Wistar rats.


METHODS
The rats were anesthetized with 1.5% sevoflurane inhalation throughout the experiment. Polyethylene catheters were introduced through the right jugular vein into the right atrium for infusion and into the right common carotid artery for blood withdrawal and mean arterial pressure monitoring.


RESULTS
Shock was induced by 50% blood withdrawal. The rats showed hypotension (mean arterial pressure = 32 +/- 10 mm Hg) and significant metabolic acidosis and hyperventilation. After 15 mins, they received HbV/rHSA, shed autologous blood (SAB), washed homologous red blood cells (wRBC) suspended in rHSA (wRBC/rHSA, [Hb] = 8.6 g/dL), or rHSA alone. The HbV/rHSA group restored mean arterial pressure to 93 +/- 8 mm Hg at 1 hr, similar to the SAB group (92 +/- 9 mm Hg), which was significantly higher compared with the rHSA (74 +/- 9 mm Hg) and wRBC/rHSA (79 +/- 8 mm Hg) groups. There was no remarkable difference in the blood gas variables between the resuscitated groups; however, two of eight rats in the rHSA group died before 6 hrs. After 6 hrs, the rHSA group showed significant ischemic changes in the right cerebral hemisphere relating to the ligation of the right carotid artery followed by cannulation, whereas the HbV/rHSA, SAB, and wRBC/rHSA groups showed less changes.


CONCLUSIONS
HbV suspended in recombinant human serum albumin provides restoration from hemorrhagic shock that is comparable with that using shed autologous blood.
Sakai
Hiromi
H
Advanced Research Institute for Science and Engineering, Waseda University, Tokyo, Japan.
Masada
Yohei
Y
Horinouchi
Hirohisa
H
Yamamoto
Manabu
M
Ikeda
Eiji
E
Takeoka
Shinji
S
Kobayashi
Koichi
K
Tsuchida
Eishun
E
D000758:Anesthesia; D000818:Animals; D001802:Blood Substitutes / Q000627:therapeutic use*; D006454:Hemoglobins / Q000008:administration & dosage*; D006801:Humans; D008081:Liposomes; D008297:Male; D011446:Prospective Studies; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins; D012151:Resuscitation; D012709:Serum Albumin; D012771:Shock, Hemorrhagic / Q000628:therapy*; D013535:Suspensions
D001802:Blood Substitutes; D006454:Hemoglobins; D008081:Liposomes; D011994:Recombinant Proteins; D012709:Serum Albumin; D013535:Suspensions