Quantitative determination of alginic acid in pharmaceutical formulations using capillary electrophoresis.
A capillary electrophoresis (CE) method has been developed and validated for the quantitative determination of alginic acid, which is used as a rafting agent in complex antacid formulations. The method involves a preliminary separation of the alginic acid from the formulation by washing the sample matrix with methanol, diluted HCl and water. This is followed by electrophoresis within a fused silica capillary using borate/boric acid buffer as the electrolyte, and the quantification is performed by a UV detector monitoring at 200 nm, where the intrinsic absorption of alginic acid is measured. An assay precision of better than 3% was achieved in intra- and interday determinations. No interference was found from the matrix of the antacid formulations.
Moore
Douglas E
DE
Faculty of Pharmacy, The University of Sydney, Sydney 2006, Australia. demoore@pharm.usyd.edu.au
Miao
William G
WG
Benikos
Con
C
D000464:Alginates / Q000032:analysis*; D000536:Aluminum Hydroxide / Q000032:analysis; D002626:Chemistry, Pharmaceutical; D004338:Drug Combinations; D004353:Drug Evaluation, Preclinical / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods; D020723:Glucuronic Acid / Q000032:analysis*; D006603:Hexuronic Acids / Q000032:analysis*; D008276:Magnesium Hydroxide / Q000032:analysis; D012841:Simethicone / Q000032:analysis
D000464:Alginates; D004338:Drug Combinations; D006603:Hexuronic Acids; C008570:aluminum hydroxide, magnesium hydroxide, simethicone drug combination; D000536:Aluminum Hydroxide; D012841:Simethicone; D020723:Glucuronic Acid; D008276:Magnesium Hydroxide