Quantification of bile acids directly from plasma by MALDI-TOF-MS.
Matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry (MS) has proved to be a useful method for the quantification of bile acids directly from plasma. Six cholic acid derivatives were selected for analysis: taurocholic acid, taurochenodeoxycholic acid, taurolithocholic acid, glycocholic acid, glycochenodeoxycholic acid, and glycolithocholic acid. Solid-phase extraction (SPE) columns were used to preconcentrate and purify the plasma samples. Calibration curves averaged from 3 days were obtained for the bile acids, and then tested for their ability to accurately determine concentrations from one measurement. In summary, a simple, rapid method has been developed for the quantification of bile salts from plasma by MALDI-MS with SPE cleanup.
Mims
DebraLynn
D
Department of Chemistry, Vanderbilt University, Nashville, TN 37214, USA.
Hercules
David
D
D001647:Bile Acids and Salts / Q000097:blood*; D002138:Calibration; D006801:Humans; D008968:Molecular Conformation; D008970:Molecular Weight; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D001647:Bile Acids and Salts