title: Tyrosine phosphorylation of BIT on photic stimulation in the rat retina.
abstract: BIT is a transmembrane glycoprotein with three immunoglobulin-like domains in its extracellular region and tyrosine phosphorylation sites in its cytosolic region. We have previously shown that BIT was tyrosine phosphorylated in the hypothalamic suprachiasmatic nucleus in response to light exposure during the dark period, and suggested that it was involved in the light entrainment of the circadian clock. To further investigate the function of BIT in the nervous system, we examined the effect of photic stimulation on its tyrosine phosphorylation in the rat retina. It was found that the tyrosine phosphorylation level of BIT in the retina was higher in the light period than in the dark period. In addition, a light stimulation during the dark period resulted in a rapid phosphorylation of BIT and a subsequent association of BIT with SHP-2. The phosphorylation state was quickly reverted when the light was turned off. The light-dependent phosphorylation of BIT was also observed in isolated cultured retinas, and this was blocked by a specific Src-family inhibitor, PP-2. Immunohistochemical study showed that BIT was highly enriched in the inner and outer plexiform layers in the retina, where the immunoreactivity to anti-SHP-2 antibody was also detected. These results suggest that tyrosine phosphorylation of BIT is involved in neuronal transmission in the retina.
authors.affiliation: Division of Protein Metabolism, Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Juri
authors.initials: J
authors.lastname: Hamada
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Okumura
authors.forename: Miho
authors.initials: M
authors.lastname: Inagaki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Taniguchi
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Nakahata
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Sano
authors.forename: Katsuya
authors.initials: K
authors.lastname: Nagai
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation; D003208:Concanavalin A; D003624:Darkness; D007150:Immunohistochemistry; D008027:Light; D008562:Membrane Glycoproteins / Q000378:metabolism*; D039842:Neural Cell Adhesion Molecule L1 / Q000378:metabolism*; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism; D010775:Photic Stimulation; D051381:Rats; D011971:Receptors, Immunologic / Q000378:metabolism*; D012160:Retina / Q000166:cytology / Q000378:metabolism / Q000502:physiology* / Q000528:radiation effects; D014443:Tyrosine / Q000378:metabolism*; D018909:src Homology Domains
chemical_list: D000943:Antigens, Differentiation; D008562:Membrane Glycoproteins; D039842:Neural Cell Adhesion Molecule L1; D011971:Receptors, Immunologic; D003208:Concanavalin A; D019000:Phosphotyrosine; D014443:Tyrosine