Quantitative analysis of strychnine and Brucine in Strychnos nux-vomica using 1H-NMR.
A quantitative analysis using (1)H-NMR (Q-NMR) has been developed for the determination of strychnine and brucine in Strychnos nux-vomica seeds and stems. The advantages of the method are that no reference alkaloids are needed for calibration curves, the quantification could be directly realized on a crude extract, strychnine and brucine could easily be distinguished, an overall profile of the preparation (including non alkaloid compounds) could be directly obtained, and a very significant time-gain could be achieved, in comparison to conventional HPLC methods, for instance.
Frédérich
Michel
M
Division of Pharmacognosy, Leiden University, Institute of Biology, Section Metabolomics, Leiden, The Netherlands.
Choi
Young Hae
YH
Verpoorte
Robert
R
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008517:Phytotherapy; D018547:Plant Stems; D012639:Seeds; D013331:Strychnine / Q000031:analogs & derivatives* / Q000737:chemistry*; D031461:Strychnos nux-vomica
C083806:brucine; D013331:Strychnine