title: Identification and subcellular localization of neuronal calcium sensor-1 (NCS-1) in human neutrophils and HL-60 cells.
abstract: Secretion in neutrophils is thought to be regulated in different ways for the different granule types. Specific granules are endowed with proteins which are related to docking and fusion events and are absent on azurophilic granules. Furthermore, even if secretion of content from all neutrophil granules is a Ca(2+)-dependent process, a higher concentration of cytosolic calcium is required for azurophilic than for specific granule secretion. In this paper we show that human neutrophils and promyelocitic cells express neuronal calcium sensor-1 (NCS-1), a calcium binding protein involved in exocytosis in various cell types. Both mRNA and protein were found in mature cells and precursors. NCS-1 is shown to be mainly associated with azurophilic granules and, therefore could play an instrumental role in the calcium-dependent secretion of azurophilic granules.
authors.affiliation: Dipartimento di Fisiologia e Patologia, Università di Trieste, Italy.
authors.forename: Cristiana
authors.initials: C
authors.lastname: Brochetta
authors.forename: Maria Giovanna
authors.initials: MG
authors.lastname: Perrotta
authors.forename: Andreas
authors.initials: A
authors.lastname: Jeromin
authors.forename: Maurizio
authors.initials: M
authors.lastname: Romano
authors.forename: Francesca
authors.initials: F
authors.lastname: Vita
authors.forename: Maria Rosa
authors.initials: MR
authors.lastname: Soranzo
authors.forename: Violetta
authors.initials: V
authors.lastname: Borelli
authors.forename: John
authors.initials: J
authors.lastname: Roder
authors.forename: Giuliano
authors.initials: G
authors.lastname: Zabucchi
mesh_terms: D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D002451:Cell Compartmentation; D002458:Cell Fractionation; D002478:Cells, Cultured; D003594:Cytoplasmic Granules / Q000737:chemistry; D018922:HL-60 Cells / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D008853:Microscopy; D051580:Neuronal Calcium-Sensor Proteins; D009479:Neuropeptides / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D009504:Neutrophils / Q000737:chemistry* / Q000648:ultrastructure; D012333:RNA, Messenger / Q000032:analysis; D014158:Transcription, Genetic
chemical_list: D002135:Calcium-Binding Proteins; D051580:Neuronal Calcium-Sensor Proteins; D009479:Neuropeptides; D012333:RNA, Messenger; C101464:frequenin calcium sensor proteins