Transgenic ginseng cell lines that produce high levels of a human lactoferrin.
In order to produce a human lactoferrin (hLf) protein in cultured plant cells, we developed Korean ginseng (Panax ginseng) cell line using an oxidative stress-inducible peroxidase (SWPA2) promoter and characterized the production of human lactoferrin in cultured cells. A construct containing a targeting signal peptide from tobacco endoplasmic reticulum fused to human lactoferrin cDNA under the control of SWPA2 promoter was engineered. Transgenic Korean ginseng cell lines that produced a recombinant hLf protein were successfully generated and confirmed by PCR and Southern blot analyses. Western blot and ELISA analyses showed that hLf protein was synthesized in the transgenic cells. The production of hLf showed a maximal level (up to 3.0% of total soluble protein) in the stationary phase of callus cultures. These results suggest that the transgenic cell lines in this study will be biotechnologically useful for the commercial production of hLf protein in cell cultures, with no need for purification.
Kwon
Suk-Yoon
SY
Laboratory of Plant Cell Biotechnology, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea.
Jo
Seung-Hyun
SH
Lee
Ok-Sun
OS
Choi
Sun-Mee
SM
Kwak
Sang-Soo
SS
Lee
Haeng-Soon
HS
D002478:Cells, Cultured; D006801:Humans; D007781:Lactoferrin / Q000096:biosynthesis* / Q000235:genetics*; D005894:Panax; D008517:Phytotherapy; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism*
D011994:Recombinant Proteins; D007781:Lactoferrin