Needle-free skin patch vaccination method for anthrax.
Three immunizations of mice with recombinant protective antigen (rPA) by transcutaneous immunization (TCI) induced long-term neutralizing antibody titers that were superior to those obtained with aluminum-adsorbed rPA. In addition, rPA alone exhibited adjuvant activity for TCI. Forty-six weeks after completion of TCI, 100% protection was observed against lethal anthrax challenge.
Matyas
Gary R
GR
Department of Membrane Biochemistry, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA. gary.matyas@na.amed.army.mil
Friedlander
Arthur M
AM
Glenn
Gregory M
GM
Little
Stephen
S
Yu
Jianmei
J
Alving
Carl R
CR
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000279:Administration, Cutaneous; D000818:Animals; D022122:Anthrax Vaccines / Q000008:administration & dosage* / Q000276:immunology; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial; D001427:Bacterial Toxins / Q000276:immunology* / Q000494:pharmacology; D004768:Enterotoxins / Q000494:pharmacology; D029968:Escherichia coli Proteins; D005260:Female; D051379:Mice; D014611:Vaccination / Q000379:methods*; D014614:Vaccines, Synthetic / Q000008:administration & dosage* / Q000276:immunology
D000276:Adjuvants, Immunologic; D022122:Anthrax Vaccines; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D014614:Vaccines, Synthetic; C030325:anthrax toxin; C027538:heat-labile enterotoxin, E coli