High levels of soluble herpes virus entry mediator in sera of patients with allergic and autoimmune diseases.
Herpes virus entry mediator (HVEM) is a newly discovered member of the tumor necrosis factor receptor (TNFR) superfamily that has a role in herpes simplex virus entry, in T cell activation and in tumor immunity. We generated mAb against HVEM and detected soluble HVEM (SHVEM) in the sera of patients with various autoimmune diseases. HVEM was constitutively expressed on CD4(+) and CD8(+) T cells, CD19(+) B cells, CD14(+) monocytes, neutrophils and dendritic cells. In three-way MLR, mAb 122 and 139 were agonists and mAb 108 had blocking activity. An ELISA was developed to detect sHVEM in patient sera. sHVEM levels were elevated in sera of patients with allergic asthma, atopic dermatitis and rheumatoid arthritis. The mAbs discussed here may be useful for studies of the role of HVEM in immune responses. Detection of soluble HVEM might have diagnostic and prognostic value in certain immunological disorders.
Jung
Hyo Won
HW
The Immunomodulation Research Center and Department of Biological Sciences, University of Ulsan, Ulsan 680-749, Korea.
La
Su Jin
SJ
Kim
Ji Young
JY
Heo
Suk Kyeung
SK
Kim
Ju Yang
JY
Wang
Sa
S
Kim
Kack Kyun
KK
Lee
Ki Man
KM
Cho
Hong Rae
HR
Lee
Hyeon Woo
HW
Kwon
Byungsuk
B
Kim
Byung Sam
BS
Kwon
Byoung Se
BS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology; D001249:Asthma / Q000097:blood / Q000276:immunology; D001327:Autoimmune Diseases / Q000097:blood* / Q000276:immunology; D002455:Cell Division; D002460:Cell Line; D003876:Dermatitis, Atopic / Q000097:blood / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006967:Hypersensitivity / Q000097:blood* / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D018124:Receptors, Tumor Necrosis Factor / Q000097:blood* / Q000276:immunology; D053240:Receptors, Tumor Necrosis Factor, Member 14; D011991:Receptors, Virus / Q000097:blood* / Q000276:immunology; D012995:Solubility
D000911:Antibodies, Monoclonal; D018124:Receptors, Tumor Necrosis Factor; D053240:Receptors, Tumor Necrosis Factor, Member 14; D011991:Receptors, Virus; C506028:TNFRSF14 protein, human; C506029:Tnfrsf14 protein, mouse