title: The distributions of mitochondria and sodium channels reflect the specific energy requirements and conduction properties of the human optic nerve head.
abstract: OBJECTIVE
  To study the normal distributions of mitochondria and voltage gated Na+ channels in the human optic nerve head in order to gain insight into the potential mechanisms of optic nerve dysfunction seen in the inherited optic neuropathies.
  
  
  METHODS
  Five fresh frozen human optic nerves were studied. Longitudinally orientated, serial cryosections of optic nerve head were cut for mitochondrial enzyme histochemistry and immunolabelling for cytochrome c oxidase (COX) subunits and voltage gated Na+ channel subtypes (Na(v) 1.1, 1.2, 1.3, and 1.6).
  
  
  RESULTS
  A high density of voltage gated Na+ channels (subtypes Na(v) 1.1, 1.3, and 1.6) in the unmyelinated, prelaminar, and laminar optic nerve was found. This distribution co-localised both with areas of high COX activity and strong immunolabelling for COX subunits I and IV.
  
  
  CONCLUSIONS
  Increased numbers of mitochondria in the prelaminar optic nerve have previously been interpreted as indicating a mechanical hold up of axoplasmic flow at the lamina cribrosa. These results suggest that this increased mitochondrial density serves the higher energy requirements for electrical conduction in unmyelinated axons in the prelaminar and laminar optic nerve and is not a reflection of any mechanical restriction. This could explain why optic neuropathies typically occur in primary inherited mitochondrial diseases such as Leber's hereditary optic neuropathy, myoclonic epilepsy with ragged red fibres (MERRF), and Leigh's syndrome. Secondary mitochondrial dysfunction has also been reported in dominant optic atrophy, Friedreich's ataxia, tobacco alcohol amblyopia, Cuban epidemic optic neuropathy, and chloramphenicol optic neuropathy. These diseases are rare but these findings challenge the traditional theories of optic nerve structure and function and may suggest an alternative approach to the study of commoner optic neuropathies such as glaucoma.
authors.affiliation: Department of Neurology, School of Neurology, Neurobiology and Psychiatry, University of Newcastle Upon Tyne, Newcastle Upon Tyne NE2 4HH, UK. m.j.barron@ncl.ac.uk
authors.forename: M J
authors.initials: MJ
authors.lastname: Barron
authors.forename: P
authors.initials: P
authors.lastname: Griffiths
authors.forename: D M
authors.initials: DM
authors.lastname: Turnbull
authors.forename: D
authors.initials: D
authors.lastname: Bates
authors.forename: P
authors.initials: P
authors.lastname: Nichols
mesh_terms: D000328:Adult; D000368:Aged; D003576:Electron Transport Complex IV / Q000378:metabolism; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D015640:Ion Channel Gating / Q000502:physiology; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism*; D036421:Nerve Fibers, Unmyelinated / Q000502:physiology; D009431:Neural Conduction / Q000502:physiology*; D009898:Optic Disk / Q000378:metabolism* / Q000502:physiology; D015222:Sodium Channels / Q000378:metabolism*
chemical_list: D015222:Sodium Channels; D003576:Electron Transport Complex IV