title: Ultrastructural study of the in vitro interaction between Biomphalaria glabrata hemocytes and Schistosoma mansoni miracidia.
abstract: Biomphalaria glabrata and Schistosoma mansoni relationship was studied by light microscopy (LM) and freeze-fracture replica technique (FFR). We observed very thin cytoplasmic extensions of hemocytes in the LM, which then surround immobilize the miracidia. FFR images showed that the contact site between hemocytes cytoplasmic extensions and the external tegumentary coat involved only superficial layers of miracidia. Numerous vacuoles and filopodia were observed in the hemocyte cytoplasm, the latter binding with those from neighboring cells. In spite of the close interfilopodia contact, no cellular junctions were seen at these sites nor between filopodia-miracidia contact areas. The observed migration of hemocytes and their disposition in layers surrounding the miracidia in vitro correspond to previous studies.
authors.affiliation: Laboratorio de Parásitos Intestinales, Centro BioMolP, Facultad de Ciencias de la Salud, Universidad de Carabobo, Brasil. waraque@uc.edu.ve
authors.forename: Wolfan
authors.initials: W
authors.lastname: Araque
authors.forename: Emilia E
authors.initials: EE
authors.lastname: Barrios
authors.forename: Pedro
authors.initials: P
authors.lastname: Rodríguez
authors.forename: Víctor S
authors.initials: VS
authors.lastname: Delgado
authors.forename: Héctor J
authors.initials: HJ
authors.lastname: Finol
mesh_terms: D000818:Animals; D001700:Biomphalaria / Q000469:parasitology*; D002465:Cell Movement; D006224:Cricetinae; D005614:Freeze Fracturing; D006434:Hemocytes / Q000469:parasitology* / Q000502:physiology / Q000648:ultrastructure; D006790:Host-Parasite Interactions; D008854:Microscopy, Electron; D012550:Schistosoma mansoni / Q000648:ultrastructure*