Ginsenosides enhance the transduction of tat-superoxide dismutase into mammalian cells and skin.
We previously reported that Tat-Cu,Zn-superoxide dismutase (Tat-SOD), a major antioxidant enzyme, can be directly transduced into mammalian cells and skin [Kwon et al. (2000); Park et al. (2002)]. To enhance the therapeutic potential of Tat-SOD in the treatment of various disorders, we screened a number of natural products for their ability to increase transduction efficiency. Ginsenosides were effective with cultured HeLa cells and enhanced the penetration of Tat-SOD into both the epidermis and the dermis of the subcutaneous layer when sprayed on mice skin. Although their mechanism of action is not fully understood we believe that ginsenosides may be useful cofactors with this antioxidant enzyme in anti-aging cosmetics or as a therapeutic protein in disorders related to reactive-oxygen species.
Kim
Dae Won
DW
Division of Life Sciences, Hallym University, Chunchon 200-702, Korea.
Eum
Won Sik
WS
Jang
Sang Ho
SH
Yoon
Chang Sik
CS
Choi
Hee Soon
HS
Choi
Soo Hyun
SH
Kim
Young Hoon
YH
Kim
So Young
SY
Lee
Eun Shil
ES
Baek
Nam-In
NI
Kwon
Hyeok Yil
HY
Choi
Jin Hi
JH
Choi
Yoon Chul
YC
Kwon
Oh-Shin
OS
Cho
Sung-Woo
SW
Han
Kyuhyung
K
Lee
Kil Soo
KS
Park
Jinseu
J
Choi
Soo Young
SY
D000818:Animals; D015696:Gene Products, tat / Q000235:genetics / Q000378:metabolism*; D036145:Ginsenosides / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D021381:Protein Transport / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000378:metabolism; D012867:Skin / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*
D015696:Gene Products, tat; D036145:Ginsenosides; D011993:Recombinant Fusion Proteins; D013482:Superoxide Dismutase