title: Difference in average survival between F344/Du and F344/N rats is not due to genetic contamination.
abstract: The average survival was found to be different between substrains of rats, F344/Du and F344/N. One of the causes of this difference could have been genetic contamination by crossbreeding with other strain(s) after the divergence of substrains. These two strains were examined by PCR-SSLP (simple sequence length polymorphism) procedure using microsatellite (Mit) markers to identify the contamination. All 288 Mit markers distributed throughout the genome exhibited no size polymorphisms between these two strains. This indicated that there was no genetic contamination between these two strains. The phenotypic difference in average survival, thus, is not due to genetic contamination.
authors.affiliation: Laboratory Animal Research Facilities, National Institute for Longevity Sciences, 36-3 Gengo, Morioka-cho, Obu 474-8522, Japan. sitanaka@nils.go.jp
authors.forename: Shin
authors.initials: S
authors.lastname: Tanaka
authors.forename: Akihito
authors.initials: A
authors.lastname: Shito
authors.forename: Norika
authors.initials: N
authors.lastname: Tamaya
authors.forename: Osamu
authors.initials: O
authors.lastname: Miyaishi
authors.forename: Masahiko
authors.initials: M
authors.lastname: Nishimura
authors.forename: Tamio
authors.initials: T
authors.lastname: Ohno