title: A digital method of sperm immobilization test: comparison to the conventional method.
abstract: Antisperm antibodies have been found in infertile patients and those causing immobilization of sperm are considered to be closely related to unexplained infertility. These antibodies are usually identified by a sperm immobilization test which involves counting motile sperm under microscope. This test is subjective as it relies on the judgement of the examiner with respect to sperm motility. In this study, we analyzed motile sperm by a digital method using Sperm Quality Analyzer. The results were compared with those obtained by the conventional method. We found that the two methods yielded identical results, with 14 of 66 samples tested being positive and 52 negative for sperm immobilizing antibodies. These results show that the digital method is objective and of value in the measurement of motile sperm in determination of sperm immobilizing antibodies.
authors.affiliation: Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan. komor615@hyo-med.ac.jp
authors.forename: Shinji
authors.initials: S
authors.lastname: Komori
authors.forename: Yukari
authors.initials: Y
authors.lastname: Hamada
authors.forename: Akiko
authors.initials: A
authors.lastname: Hasegawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsubamoto
authors.forename: Isao
authors.initials: I
authors.lastname: Horiuchi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tanaka
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kasumi
authors.forename: Minoru
authors.initials: M
authors.lastname: Shigeta
authors.forename: Koji
authors.initials: K
authors.lastname: Koyama
mesh_terms: D000372:Agglutination Tests / Q000379:methods*; D000906:Antibodies / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D009716:Numerical Analysis, Computer-Assisted; D013073:Sperm Agglutination; D013081:Sperm Motility; D013094:Spermatozoa / Q000276:immunology
chemical_list: D000906:Antibodies