Cell death evaluation in benzo[a]pyrene-transformed human breast epithelial cells after microcell-mediated transfer of chromosomes 11 and 17.
The incidence of apoptosis and nuclear instability, including the incidence of catastrophic death, were investigated in benzo[a]pyrene (BP)-transformed human breast epithelial cells (BP1-E cell line) after microcell-mediated transfer of chromosomes 11 and 17. Since the introduction of normal chromosomes 11 and 17 into tumorigenic human breast BP1-E cells reverts some of these cells' characteristics (especially those affected by microsatellite instabilities and loss of heterozygosity) to those of parental non-transformed MCF-10F cells, it was expected that the cell death rates would also be affected by this treatment. The transfer of the mentioned chromosomes, especially chromosome 17, to tumorigenic BP1-E cells increased the apoptotic ratios and decreased the nuclear instability ratios, thus showing that the microsatellite instability and loss of heterozygosity induced by BP in these chromosomes of MCF-10F cells affect the control of cell death mechanisms.
Mello
Maria Luiza S
ML
Department of Cell Biology, Institute of Biology, UNICAMP, 13084-971 Campinas (SP), Brazil. mlsmello@unicamp.br
Barbisan
Luis Fernando
LF
Lareef
Mohamed H
MH
Russo
Jose
J
Vidal
Benedicto de Campos
Bde C
D017209:Apoptosis / Q000187:drug effects*; D001564:Benzo(a)pyrene / Q000494:pharmacology*; D001940:Breast / Q000166:cytology / Q000187:drug effects*; D002461:Cell Line, Transformed; D002880:Chromosomes, Human, Pair 11; D002886:Chromosomes, Human, Pair 17; D004847:Epithelial Cells / Q000187:drug effects; D006801:Humans
D001564:Benzo(a)pyrene