title: Trypanocidal constituents in plants 4. Withanolides from the aerial parts of Physalis angulata.
abstract: The constituents of the aerial parts of Physalis angulata (Solanaceae) were investigated based on the plant's trypanocidal activity against epimastigotes of Trypanosoma cruzi, the etiologic agent for Chagas' disease. Four new withanolides were isolated, along with six known ones, from the active fraction. Their structures were determined by spectroscopic analysis. Trypanocidal activity against trypomastigotes, an infectious form of T. cruzi, was also estimated, as well as cytotoxic activity against human uterine carcinoma (HeLa) cells in vitro. Evaluation of trypanocidal activity using the colorimetric reagent Cell Counting Kit-8 was also examined.
authors.affiliation: Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Nagafuji
authors.forename: Hikaru
authors.initials: H
authors.lastname: Okabe
authors.forename: Hiroshige
authors.initials: H
authors.lastname: Akahane
authors.forename: Fumiko
authors.initials: F
authors.lastname: Abe
mesh_terms: D000818:Animals; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000494:pharmacology; D066298:In Vitro Techniques; D020128:Inhibitory Concentration 50; D032321:Physalis / Q000737:chemistry*; D035261:Plant Components, Aerial / Q000737:chemistry*; D028321:Plant Preparations / Q000627:therapeutic use; D013329:Structure-Activity Relationship; D014344:Trypanocidal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000187:drug effects
chemical_list: D028321:Plant Preparations; D014344:Trypanocidal Agents; C433552:withanolide 2; C493691:withanolide-10; C493689:withanolide-5; C493690:withanolide-9; D004875:Ergosterol