[The inhibitory effect of Lu-Duo-Wei on carbon disulfide-induced generation of hydroxyl radicals].
OBJECTIVE
To study the scavenging effects of Lu-Duo-Wei, thiourea, superoxide dismutase, and sodium azide on carbon disulfide-induced generation of hydroxyl radicals.


METHODS
Phenanthroline-CuSO(4)-Vit C-H(2)O(2) chemiluminescence system (PHEN system) containing alcohol was established to probe the influence of various concentrations of carbon disulfide on hydroxyl radicals emission intensity and the scavenging effects of Lu-Duo-Wei and other antioxidants on carbon disulfide-induced hydroxyl radicals were observed.


RESULTS
The average emission intensity of PHEN system containing alcohol appeared lower luminescence [91.03 x 10(3) (cp6s)] and longer time (75 s) to get the peak than the system without alcohol [96.11 x 10(3) (cp6s), 55 s]. The specific scavenger of hydroxyl radical, thiourea, showed clear inhibitory effect on the system. Carbon disulfide in the range of 40 - 160 mmol/L promoted the generation of hydroxyl radical, however, this effect could be efficiently inhibited by thiourea. 160 mmol/L carbon disulfide in PHEN system without copper seemed as an activator to promote the luminescence, while in PHEN system withdrawing phenanthroline appeared some weak action of luminescence agent at low concentration. Meanwhile, Lu-Duo-Wei may efficiently scavenge hydroxyl radicals induced by carbon disulfide in PHEN system but superoxide dismutase and sodium azide had little effects on the system.


CONCLUSIONS
Carbon disulfide may induce PHEN system to generate hydroxyl radicals and Lu-Duo-Wei may efficiently scavenge these free radicals and play an important role in protection against oxidative injury induced by carbon disulfide.
Jian
Le
L
Institute of Occupational and Environmental Health, Zhejiang University, Hangzhou 310006, China.
Lang
Hui-qing
HQ
D002246:Carbon Disulfide / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D008163:Luminescent Measurements; D010618:Phenanthrolines / Q000737:chemistry; D013482:Superoxide Dismutase / Q000494:pharmacology; D013890:Thiourea / Q000494:pharmacology
D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D010618:Phenanthrolines; C403260:lu-duo-wei; D017665:Hydroxyl Radical; D013482:Superoxide Dismutase; D013890:Thiourea; D002246:Carbon Disulfide