title: [Effect of handportable mobiletelephone microwave radiation on rat central neuron apoptosis].
abstract: OBJECTIVE
  To investigate the effect of handportable mobiletelephone microwave radiation on rat central nervous system by setting up rat model.
  
  
  METHODS
  80 healthy male SD rats (weighed about 200 g) were divided into 4 groups at random: control, radiation, decranium, decranium + radiation. TUNEL method was adopted used to detect the apoptosis of neurons after irradiation, then immunohistochemistry was used to detect Bcl-2, Bax expression in all brain tissue.
  
  
  RESULTS
  TUNEL positive rate, Bax and Bcl-2 positive cell numbers could be found in decranium + radiation group [(26.45 +/- 9.27)%, (23.5 +/- 3.58), (11.1 +/- 2.55) respectively]. There were significant differences among control [(9.59 +/- 2.55)%, 14.2 +/- 2.46, 7.0 +/- 1.14 respectively], decranium group [(9.52 +/- 1.93)%, 15.5 +/- 1.77, 7.4 +/- 1.76], radiation group [(10.04 +/- 3.62)%, 15.9 +/- 2.02, 7.2 +/- 1.07] (P < 0.01). But the difference was not found in the ratio of Bax to Bcl-2.
  
  
  CONCLUSIONS
  Microwave radiation did not affect the intact rat, but did promote the occurrence of neuron apoptosis in cranial defect rat. Bax, Bcl-2 gene participated in regulation of apoptosis. The intact cranium may be an important factor to protect the neurons against handportable mobiletelephone microwave radiation to some extent.
authors.affiliation: The Second Hospital, College of Medical Science, Zhejiang University, Hangzhou 310009, China.
authors.forename: Wei-guo
authors.initials: WG
authors.lastname: Liu
authors.forename: Xiao-feng
authors.initials: XF
authors.lastname: Yang
authors.forename: Yong-jian
authors.initials: YJ
authors.lastname: Zhu
authors.forename: Hong
authors.initials: H
authors.lastname: Shen
authors.forename: Xiu-yi
authors.initials: XY
authors.lastname: Jiang
authors.forename: Shi-ting
authors.initials: ST
authors.lastname: Lu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects*; D001921:Brain / Q000473:pathology / Q000528:radiation effects*; D020287:In Situ Nick-End Labeling; D008297:Male; D008872:Microwaves / Q000009:adverse effects*; D009474:Neurons / Q000473:pathology / Q000528:radiation effects*; D011518:Proto-Oncogene Proteins / Q000032:analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D051028:bcl-2-Associated X Protein
chemical_list: C494828:Bax protein, rat; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein