title: MALDI-TOF mass spectrometry: a versatile tool for high-performance DNA analysis.
abstract: Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) has developed during the past decade into a versatile tool for biopolymer analysis. The aim of this review is to summarize this development and outline the applications, which have been enabled for routine use in the field of nucleic acid analysis. These include the analysis of mutations, the resequencing of amplicons with a known reference sequence, and the quantitative analysis of gene expression and allelic frequencies in complex DNA mixtures.
authors.affiliation: Sequenom, Inc., Johns Hopkins Court, San Diego, CA 92121, USA. cjurinke@sequenom.com
authors.forename: Christian
authors.initials: C
authors.lastname: Jurinke
authors.forename: Paul
authors.initials: P
authors.lastname: Oeth
authors.forename: Dirk
authors.initials: D
authors.lastname: van den Boom
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D004252:DNA Mutational Analysis / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D004247:DNA