ydk1-D, an auxin-responsive GH3 mutant that is involved in hypocotyl and root elongation.
To study the GH3 gene family of Arabidopsis, we investigated a flanking sequence database of Arabidopsis activation-tagged lines. We found a dwarf mutant, named yadokari 1-D (ydk1-D), that had a T-DNA insertion proximal to a GH3 gene. ydk1-D is dominant and has a short hypocotyl not only in light but also in darkness. Moreover, ydk1-D has a short primary root, a reduced lateral root number, and reduced apical dominance. A GH3 gene, named YDK1, was upregulated in ydk1-D, and YDK1 transgenic plants showed the ydk1-D phenotype. YDK1 gene expression was induced by exogenously applied auxin and regulated by auxin-response factor (ARF)7. In addition, YDK1 gene expression was downregulated by blue and far-red (FR) lights. Strong promoter activity of YDK1 was observed in roots and flowers. These results suggest that YDK1 may function as a negative component in auxin signaling by regulating auxin activity.
Takase
Tomoyuki
T
Graduate School of Integrated Science, Yokohama City University, 22-2 Seto, Kanazawaku, Yokohama, Kanagawa 236-0027, Japan.
Nakazawa
Miki
M
Ishikawa
Akie
A
Kawashima
Mika
M
Ichikawa
Takanari
T
Takahashi
Naoki
N
Shimada
Hiroaki
H
Manabe
Katsushi
K
Matsui
Minami
M
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D018546:Hypocotyl / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008027:Light; D005810:Multigene Family; D010641:Phenotype; D010937:Plant Growth Regulators / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D030821:Plants, Genetically Modified
D029681:Arabidopsis Proteins; C425524:DFL1 protein, Arabidopsis; C481444:DFL2 protein, Arabidopsis; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; C486358:YDK1 protein, Arabidopsis