title: Quantitative mRNA analysis of IL-1 gene system in human testis.
abstract: OBJECTIVE
  There is a growing body of evidence that interleukins exhibit modulatory activity on development of reproductive cells. In this context, there appears to be a role for IL-1, which is also produced in human testis. We have analysed transcripts of IL-1 gene system (IL-1alpha, IL-1beta, IL-1RI, IL-1RII and IL-1RA) to evaluate the possible link between the level of gene(s) transcription and their function.
  
  
  METHODS
  To determine the activity of gene transcription, a quantitative PCR with isotopic and/or nonisotopic detection was applied.
  
  
  CONCLUSIONS
  We have detected differential expression of IL-1alpha and IL-1beta genes in separate functional compartments of a male gonad. A strong expression of IL-1alpha gene in an intratubular cell fraction was shown, while the IL-1beta expression seemed to be dominant in extratubular compartment of the male gonad. Abundant amounts of IL-1RA mRNA in gametogenic cells fraction slightly higher than in interstitium have also been found. IL-1RA is the most important regulatory molecule in IL-1 system, which down-regulates activity of both interleukins. Looking more closely at gene(s) differential expression it appears that IL-1alpha can be preferentially down-regulated by IL-1RA gene in intratubular fraction while the IL-1beta, through the "false" IL-1RII receptor in the interstitium. Genes coding for both receptors (IL-1RI and IL-1RII) showed, however, relatively low levels of transcription in both studied compartments. IL-1 genes system creates a complex intragonadal environment and the function of these genes is reflected by their respective distribution in the two main functional compartments of the testis.
authors.affiliation: Institute of Human Genetics, Polish Academy of Sciences, Poznań, Poland.
authors.forename: D
authors.initials: D
authors.lastname: Fiszer
authors.forename: N
authors.initials: N
authors.lastname: Rozwadowska
authors.forename: A
authors.initials: A
authors.lastname: Lukaszyk
authors.forename: R
authors.initials: R
authors.lastname: Słomski
authors.forename: M
authors.initials: M
authors.lastname: Kurpisz
mesh_terms: D001483:Base Sequence; D005786:Gene Expression Regulation; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D008297:Male; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger