Time-course study of SCG10 mRNA levels associated with LTP induction and maintenance in the rat Schaffer-CA1 pathway in vivo.
The maintenance of long-term potentiation (LTP) depends on altered gene expression. Previously, we found the expression of neuronal growth associated protein SCG10, which is involved in neurite outgrowth and neural regeneration, was up-regulated by LTP induction in the rat hippocampal Schaffer-collateral CA1 pathway. Here we studied the temporal expression pattern of SCG10 mRNA after LTP induction using permanently implanted electrodes in the same CA1 pathway. The real-time RT-PCR showed that both SCG10 mRNA 1 and 2 kb forms were increased at the 3 h, but not at 1 or 24 h. In situ hybridization revealed an increase of SCG10 2 kb mRNA level in ipsilateral CA3 and CA1 areas, but not their contralateral counterparts or either side of dentate gyrus. These results suggest that SCG10 may play a role in the maintenance of synaptic plasticity through a transient regulation of microtubule dynamics, which facilitates the structural remodeling of the presynaptic element during the consolidation period.
Peng
Haixiang
H
Cajal Neuroscience Institute, Department of Biology, University of Texas at San Antonio, 6900 North Loop 1604 West, San Antonio, TX 78249-0662, USA. hpeng@lonestar.utsa.edu
Derrick
Brian E
BE
Martinez
Joe L
JL
D000818:Animals; D001345:Autoradiography / Q000379:methods; D002352:Carrier Proteins; D004558:Electric Stimulation; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D007839:Functional Laterality; D006624:Hippocampus / Q000166:cytology*; D017403:In Situ Hybridization / Q000379:methods; D017774:Long-Term Potentiation / Q000502:physiology* / Q000528:radiation effects; D008297:Male; D008565:Membrane Proteins; D008868:Microtubule Proteins; D009414:Nerve Growth Factors / Q000378:metabolism*; D009434:Neural Pathways / Q000502:physiology*; D009474:Neurons / Q000378:metabolism* / Q000502:physiology; D010879:Piperazines / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013997:Time Factors
D002352:Carrier Proteins; D018691:Excitatory Amino Acid Antagonists; D008565:Membrane Proteins; D008868:Microtubule Proteins; D009414:Nerve Growth Factors; D010879:Piperazines; D012333:RNA, Messenger; C512848:Stmn2 protein, rat; C050592:3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid