Candidate regions of tumor suppressor locus on chromosome 9q31.1 in gastric cancer.
Loss of heterozygosity (LOH) is an important event of tumorigenesis. In gastric cancer, we found a novel region of LOH in chromosome 9q having about 800 kb deletions at 9q31.1. The microsatellite marker D9S938 in that region exhibiting the highest LOH frequency, 56.5%. In addition, the LOH at 9q31.1 did not show any relationship to either histologic types or stages of gastric cancers, and several genes were predicted in the remaining allele by in silico methods. These data suggest that the deletion at 9q31.1 would be common in both differentiated-type and undifferentiated-type gastric cancers. Furthermore, this deletion was found in the primary tumors of early-stage gastric cancer, indicating that loss of function of predicted genes appears to be associated with the tumorigenesis of gastric cancer.
Kakinuma
Naoto
N
Novartis Pharma Tsukuba Research Institute, Ibaraki, Japan.
Kohu
Kazuyoshi
K
Sato
Masaaki
M
Yamada
Tatsuya
T
Nakajima
Motowo
M
Akiyama
Tetsu
T
Ohwada
Susumu
S
Shibanaka
Yasuhiko
Y
D002454:Cell Differentiation; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9 / Q000648:ultrastructure*; D017931:DNA Primers / Q000494:pharmacology; D017353:Gene Deletion; D016147:Genes, Tumor Suppressor; D016678:Genome; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D008957:Models, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D012984:Software; D013274:Stomach Neoplasms / Q000235:genetics*
D017931:DNA Primers; D012333:RNA, Messenger