Characterization of Gicerin/MUC18/CD146 in the rat nervous system.
Gicerin is a cell adhesion molecule of an immunoglobulin (Ig) superfamily isolated from a chicken. It shows homophilic and heterophilic binding activities and has two isoforms. s-Gicerin which has small cytoplasmic domain and the same extracellular domain as l-gicerin shows stronger cell adhesion activity. In the chick nervous system, gicerin expression is only observed in the developmental stage when neurons extend neurites and migrate. In other tissues, gicerin participates in the tissue regeneration or oncogenesis. In this report, we identified two isoforms of rat gicerin corresponding to chicken and we concluded that gicerin is a homologue of human CD146/MUC18/MCAM. Next we generated antibody to characterize a rat gicerin in the nervous system. Gicerin is expressed in the hippocampal cells, Purkinje cells, and sensory neurons of a spinal chord of an adult rat, while expressed most abundantly in the lung. In addition to this, its expression in the hippocampus was increased by electroconvulsive shock, suggesting some role in the mature nervous system. And we also showed neurite promotion activity of gicerin from hippocampal neurons.
Taira
Eiichi
E
Department of Pharmacology, Osaka University School of Medicine, Suita, Osaka, Japan. etaira@pharma1.med.osaka-u.ac.jp
Kohama
Keiko
K
Tsukamoto
Yasuhiro
Y
Okumura
Shigeki
S
Miki
Naomasa
N
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D030161:Avian Proteins; D015152:Blotting, Northern; D015153:Blotting, Western; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D051929:CD146 Antigen; D002352:Carrier Proteins / Q000737:chemistry / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000502:physiology*; D017931:DNA Primers; D004622:Embryo, Mammalian; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008562:Membrane Glycoproteins / Q000737:chemistry; D008969:Molecular Sequence Data; D019006:Neural Cell Adhesion Molecules; D016501:Neurites / Q000378:metabolism; D016133:Polymerase Chain Reaction; D020033:Protein Isoforms / Q000737:chemistry / Q000502:physiology; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000502:physiology; D012640:Seizures / Q000503:physiopathology; D017386:Sequence Homology, Amino Acid
D015703:Antigens, CD; D030161:Avian Proteins; D051929:CD146 Antigen; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D017931:DNA Primers; C497902:MCAM protein, human; C512746:Mcam protein, rat; D008562:Membrane Glycoproteins; D019006:Neural Cell Adhesion Molecules; D020033:Protein Isoforms; D011993:Recombinant Fusion Proteins; C086935:gicerin protein, Gallus gallus