Bacteriocin of Enterococcus from lactoserum able to cause oxidative stress in Staphylococcus aureus.
The effect of a bacteriocin of Enterococcus on the oxidative metabolism of sensitive bacteria was investigated through the detection of oxidative stress by chemiluminescence (CL). The bacteriocin named EntB was purified to study the action on Staphylococcus aureus isolated from cosmetic. Chromatographic separation of EntB indicated different states of oligomerization with molecular weights multiple of 12,000Da monomeric form. The monomer purified by ion exchange was studied in its capacity to affect the oxidative metabolism of S. aureus, which showed increase of anion superoxide (O(2)(-)) when incubated with EntB. This effect was compared to the action of EntB on leukocytes as an assay of toxicity. EntB did not generate significant oxidative stress in leukocytes. Pyoverdin, a leukotoxic pigment of Pseudomonas fluorescens, was taken as reference, and it was found that this pigment caused similar oxidative stress to EntB in S. aureus; however, pyoverdin generated high production of anion superoxide (O(2)(-)) in leukocytes, while EntB did not increase the level of O(2)(-).
Eraso
Alberto Jorge
AJ
Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Fisicoquímica y Naturales, Universidad Nacional de Río Cuarto, Argentina. aeraso@exa.unrc.edu.ar
Inés
Albesa
A
D001430:Bacteriocins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D016983:Enterococcus / Q000737:chemistry*; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007106:Immune Sera / Q000737:chemistry*; D008163:Luminescent Measurements; D008970:Molecular Weight; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects; D009842:Oligopeptides; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D010860:Pigments, Biological / Q000494:pharmacology; D011551:Pseudomonas fluorescens / Q000737:chemistry / Q000235:genetics; D013211:Staphylococcus aureus / Q000187:drug effects / Q000378:metabolism*; D013481:Superoxides / Q000032:analysis / Q000378:metabolism
D001430:Bacteriocins; D007106:Immune Sera; D009842:Oligopeptides; D010860:Pigments, Biological; C018577:lactoserum; D013481:Superoxides; C042453:pyoverdin