Degradation of xenobiotic substances using sulfate-reducing bacteria in a UASB reactor.
An upflow anaerobic sludge blanket (UASB) reactor was successfully applied to continuous degradation of ferric ethylene diamine tetraacetate (Fe-EDTA) as a typical xenobiotic substance contained in photo-processing wastewater. The sludge in the UASB reactor had an abundance of sulfate-reducing bacteria (SRB), which had been anaerobically cultivated in a sulfate-rich culture medium including Fe-EDTA and yeast-extract as the carbon sources. Since the prominent reductions of Fe-EDTA and sulfate ion were observed, the contribution of SRB to Fe-EDTA degradation in the UASB reactor was confirmed. The aggregated sludge in the UASB reactor became gradually large reaching steady state with an equivalent diameter of 60-90 microm after 124 days operation. An increase of the amount of yeast extract addition to feed solution improved the Fe-EDTA removal efficiency up to 90%. Moreover, the combination of ozone treatment with SRB treatment further improved removal efficiency of total organic carbon (TOC) in an actual photo-processing wastewater composed of fixing and developing wastes.
Tsuneda
S
S
Department of Chemical Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan. stsuneda@waseda.jp
Shiono
T
T
Nakamura
K
K
Dogan
T
T
Hirata
A
A
D001419:Bacteria; D019149:Bioreactors; D002614:Chelating Agents / Q000737:chemistry; D004492:Edetic Acid / Q000737:chemistry; D007220:Industrial Waste; D007501:Iron / Q000737:chemistry; D010083:Oxidants, Photochemical / Q000737:chemistry; D010126:Ozone / Q000737:chemistry; D013455:Sulfur / Q000378:metabolism; D014865:Waste Disposal, Fluid / Q000379:methods*; D018508:Water Purification / Q000379:methods*; D015262:Xenobiotics / Q000378:metabolism*; D015003:Yeasts
D002614:Chelating Agents; D007220:Industrial Waste; D010083:Oxidants, Photochemical; D015262:Xenobiotics; D010126:Ozone; D013455:Sulfur; D004492:Edetic Acid; D007501:Iron