title: Evaluation of metallothionein as a biomarker of single and combined Cd/Cu exposure in Dreissena polymorpha.
abstract: The effects of metal mixture (Cd+Cu) versus single-metal exposure on total MT response and bioaccumulation were investigated in the freshwater bivalve Dreissena polymorpha. A two-month exposure period, including two levels of contamination, was chosen for each of the two metals: 5, 10 microg/L for Cu, and 2, 20 microg/L for Cd, with mixtures of, respectively, 5 microg/L Cu+2 microg/L Cd, 5 microg/L Cu+20 microg/L Cd, 10 microg/L Cu+2 microg/L Cd, and 10 microg/L Cu+20 microg/L Cd. Total MT contents were assessed by an Ag-saturation method, and metals contents were determined by atomic absorption spectrometry. Results at the whole-organism level showed a significant and early increase of total MT biosynthesis after exposure to Cd. This increase was significantly correlated with Cd bioaccumulation. By contrast, Cu did not modify total MT response, and mussels limited Cu bioaccumulation. The mixture either did not influence or only weakly influenced metal accumulation and MT response to Cu and Cd after long-term exposure. Our results suggest that the form of MT existing in D. polymorpha was not Cu-inducible. This could limit the use of MT in D. polymorpha as a biomarker of heavy metal pollution in freshwater ecosystems.
authors.affiliation: UMR INRA-DGER Métabolisme et Toxicologie Comparés des Xénobiotiques, Ecole NationaleVétérinaire de Lyon, 1, av. Bourgelat, BP 83, F-69280 Marcy l'Etoile, France. s.lecoeur@vet-lyon.fr
authors.forename: S
authors.initials: S
authors.lastname: Lecoeur
authors.forename: B
authors.initials: B
authors.lastname: Videmann
authors.forename: Ph
authors.initials: P
authors.lastname: Berny
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D049872:Bivalvia; D002104:Cadmium / Q000493:pharmacokinetics*; D002105:Cadmium Poisoning / Q000662:veterinary*; D003300:Copper / Q000493:pharmacokinetics / Q000506:poisoning*; D004347:Drug Interactions; D004781:Environmental Exposure; D008668:Metallothionein / Q000032:analysis*; D014018:Tissue Distribution; D014873:Water Pollutants / Q000493:pharmacokinetics / Q000506:poisoning*
chemical_list: D015415:Biomarkers; D014873:Water Pollutants; D002104:Cadmium; D003300:Copper; D008668:Metallothionein