title: B7-DC regulates asthmatic response by an IFN-gamma-dependent mechanism.
abstract: B7-H1 (PD-L1) and B7-DC (PD-L2) are the ligands for programmed death-1 (PD-1), which is a member of the CD28/CTLA-4 family and has been implicated in peripheral tolerance. We investigated the roles of B7-H1 and B7-DC in a murine OVA-induced allergic asthma model. B7-H1 was constitutively expressed on dendritic cells, macrophages, B cells, and T cells in the lungs of naive mice, and its expression could be dramatically increased after allergen challenge. In contrast, B7-DC expression was scarcely expressed on dendritic cells in naive mice, but was up-regulated after allergen challenge, although the up-regulation of B7-DC expression on macrophages was minimal. Treatment of mice with anti-B7-DC mAb at the time of allergen challenge, but not at the time of sensitization, significantly increased their airway hyper-reactivity and eosinophilia. Such treatment also resulted in the increased production of IL-5 and IL-13, and decreased IFN-gamma production in the lungs and draining lymph node cells. These changes were diminished when mice were depleted of IFN-gamma by anti-IFN-gamma mAb pretreatment. Interestingly, treatment with anti-B7-H1 or anti-PD-1 mAb did not significantly affect the asthmatic response. These results suggest a unique role for B7-DC in the regulation of asthmatic response through an IFN-gamma-dependent, but PD-1-independent, mechanism.
authors.affiliation: Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Koichiro
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Inoue
authors.forename: Takako
authors.initials: T
authors.lastname: Nakano
authors.forename: Miyuki
authors.initials: M
authors.lastname: Tsuda
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yoshiura
authors.forename: Satoru
authors.initials: S
authors.lastname: Fukuyama
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Tsushima
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Hoshino
authors.forename: Hisamichi
authors.initials: H
authors.lastname: Aizawa
authors.forename: Hisaya
authors.initials: H
authors.lastname: Akiba
authors.forename: Drew
authors.initials: D
authors.lastname: Pardoll
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hara
authors.forename: Hideo
authors.initials: H
authors.lastname: Yagita
authors.forename: Miyuki
authors.initials: M
authors.lastname: Azuma
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Nakanishi
mesh_terms: D000485:Allergens / Q000008:administration & dosage / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000943:Antigens, Differentiation / Q000096:biosynthesis / Q000276:immunology; D001249:Asthma / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D018122:B7-1 Antigen / Q000096:biosynthesis / Q000276:immunology / Q000494:pharmacology / Q000502:physiology*; D060890:B7-H1 Antigen; D001798:Blood Proteins; D016535:Bronchial Hyperreactivity / Q000276:immunology / Q000503:physiopathology; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D004195:Disease Models, Animal; D007371:Interferon-gamma / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000502:physiology*; D018793:Interleukin-13 / Q000096:biosynthesis; D015848:Interleukin-5 / Q000096:biosynthesis; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism; D008168:Lung / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D010455:Peptides; D061027:Programmed Cell Death 1 Ligand 2 Protein; D061026:Programmed Cell Death 1 Receptor; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D015854:Up-Regulation / Q000276:immunology
chemical_list: D000485:Allergens; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D018122:B7-1 Antigen; D060890:B7-H1 Antigen; D001798:Blood Proteins; C498919:Cd274 protein, mouse; D018793:Interleukin-13; D015848:Interleukin-5; D008562:Membrane Glycoproteins; C491383:Pdcd1 protein, mouse; C556978:Pdcd1lg2 protein, mouse; D010455:Peptides; D061027:Programmed Cell Death 1 Ligand 2 Protein; D061026:Programmed Cell Death 1 Receptor; D007371:Interferon-gamma; D010047:Ovalbumin