Determination of aliphatic amines using N-succinimidyl benzoate as a new derivatization reagent in gas chromatography combined with solid-phase microextraction.
A simple, selective and sensitive approach was developed for the quantitation of aliphatic amines in lake water applying a new reagent (N-succinimidyl benzoate, SIBA), synthesized in the laboratory of the authors. Derivatization of the n-C1-C6 aliphatic monoamines and dimethylamine in aqueous solution with SIBA was followed by headspace solid-phase microextraction (SPME). Derivatives were identified by gas chromatography-mass spectrometry and determined by gas chromatography-flame ionization detection. Both derivatization and SPME conditions have been optimized. Derivatizations were performed in borate buffer (pH 8.8), at 60 degrees C for 22 min. SPME was carried out from saturated sodium chloride solution, at 80 degrees C for 60 min, desorption at 250 degrees C for 2 min. Detection limit of derivatized amines proved to be 0.13-7.2 nmol/l, while recovery of amines from lake water samples, in the concentration range of 100-200 microg/l, varied from 94.1 to 102.7%.
Zhao
Yuan-Yuan
YY
College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072, China.
Cai
Ling-Shuang
LS
Jing
Zhi-Zhong
ZZ
Wang
Hong
H
Yu
Jian-Xin
JX
Zhang
Hua-Shan
HS
D000588:Amines / Q000032:analysis*; D001565:Benzoates / Q000737:chemistry*; D002849:Chromatography, Gas / Q000379:methods*; D007202:Indicators and Reagents / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D013388:Succinimides / Q000737:chemistry*
D000588:Amines; D001565:Benzoates; D007202:Indicators and Reagents; C488878:N-succinimidyl benzoate; D013388:Succinimides