HIV-1 cell to cell transfer across an Env-induced, actin-dependent synapse.
Direct cell-cell transfer is an efficient mechanism of viral dissemination within an infected host, and human immunodeficiency virus 1 (HIV-1) can exploit this mode of spread. Receptor recognition by HIV-1 occurs via interactions between the viral surface envelope glycoprotein (Env), gp120, and CD4 and a chemokine receptor, CCR5 or CXCR4. Here, we demonstrate that the binding of CXCR4-using HIV-1-infected effector T cells to primary CD4(+)/CXCR4(+) target T cells results in rapid recruitment to the interface of CD4, CXCR4, talin, and lymphocyte function-associated antigen 1 on the target cell, and of Env and Gag on the effector cell. Recruitment of these membrane molecules into polarized clusters was dependent on Env engagement of CD4 and CXCR4 and required remodelling of the actin cytoskeleton. Transfer of Gag from effector to target cell was observed by 1 h after conjugate formation, was independent of cell-cell fusion, and was probably mediated by directed virion fusion with the target cell. We propose that receptor engagement by Env directs the rapid, actin-dependent recruitment of HIV receptors and adhesion molecules to the interface, resulting in a stable adhesive junction across which HIV infects the target cell.
Jolly
Clare
C
The Sir William Dunn School of Pathology, University of Oxford, UK.
Kashefi
Kirk
K
Hollinshead
Michael
M
Sattentau
Quentin J
QJ
D000199:Actins / Q000378:metabolism*; D015704:CD4 Antigens / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism / Q000821:virology; D002460:Cell Line; D015699:HIV Envelope Protein gp120 / Q000502:physiology*; D015658:HIV Infections / Q000378:metabolism / Q000821:virology; D015497:HIV-1 / Q000472:pathogenicity* / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D007365:Intercellular Junctions / Q000378:metabolism* / Q000821:virology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D016253:Microscopy, Immunoelectron; D019718:Receptors, CXCR4 / Q000378:metabolism
D000199:Actins; D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D016169:Lymphocyte Function-Associated Antigen-1; D019718:Receptors, CXCR4