Morphological and molecular effects of 20-hydroxyecdysone and its agonist tebufenozide on CF-203, a midgut-derived cell line from the spruce budworm, Choristoneura fumiferana.
The morphological and molecular responses of a midgut-derived cell line of the spruce budworm, Choristoneura fumiferana, to 20-hydroxyecdysone (20E) and the nonsteroidal ecdysone agonist, tebufenozide (RH-5992), were investigated. The cells responded to these compounds by clumping, generating filamentous extensions, increased mortality and expression of the transcription factor, Choristoneura hormone receptor 3 (CHR3). This cell line can be used as a model system to study the mode of action of ecdysone and its agonists. With subsequent passaging in ecdysteroid-containing medium, the degree of clumping increased and the clumping could not be reversed by subculturing in ecdysteroid-free medium. Cell numbers of the adapted cell lines in 20E and RH-5992 containing media were not significantly decreased, compared to the control, but both cell lines accumulated less (14)C-labeled RH-5992 and lost the capability of expressing CHR3 in response to these compounds. Taken together, the cell lines appeared to develop a mechanism to adapt to the toxic effects of these compounds. Arch. Insect Biochem. Physiol. 55:68-78, 2004.
Hu
Wenqi
W
Great Lakes Forestry Centre, Canadian Forest Service, Natural Resources Canada, Sault Ste. Marie, Ontario, Canada.
Cook
Barbara J
BJ
Ampasala
Dinakara R
DR
Zheng
Sichun
S
Caputo
Guido
G
Krell
Peter J
PJ
Retnakaran
Arthur
A
Arif
Basil M
BM
Feng
Qili
Q
D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects; D002452:Cell Count; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D004268:DNA-Binding Proteins; D004064:Digestive System / Q000166:cytology; D004305:Dose-Response Relationship, Drug; D004441:Ecdysterone / Q000819:agonists* / Q000494:pharmacology*; D006834:Hydrazines / Q000378:metabolism / Q000494:pharmacology*; D007300:Insect Control; D019476:Insect Proteins; D007306:Insecticides / Q000494:pharmacology; D007915:Lepidoptera / Q000166:cytology* / Q000187:drug effects*; D008858:Microscopy, Phase-Contrast; D018010:Receptors, Invertebrate Peptide / Q000096:biosynthesis; D015534:Trans-Activators
C104701:CHR3 protein, Choristoneura fumiferana; D004268:DNA-Binding Proteins; D006834:Hydrazines; D019476:Insect Proteins; D007306:Insecticides; D018010:Receptors, Invertebrate Peptide; D015534:Trans-Activators; D004441:Ecdysterone; C082026:tebufenozide