Coronin-1 expression in T lymphocytes: insights into protein function during T cell development and activation.
Coronin has been described as an actin-binding protein of Dictyostelium discoideum, and it has been demonstrated to play a role in cell migration, cytokinesis and phagocytosis. Coronin-related proteins are found in many eukaryotic species, including Coronin-1 in mammals whose expression is enriched in the hematopoietic tissues. Here, we characterize Coronin-1 gene and protein expression in mouse embryonic and adult T lymphocytes. Coronin-1 is expressed throughout T cell ontogeny and in peripheral alphabeta T cells. Expression varies along thymic cell development, with maximum levels observed in embryonic early thymocytes and, in the adults, the selected TCRalphabeta(+) single-positive thymocytes. Subcellular localization analysis indicates that Coronin-1 is in equilibrium between the cytosol and the cell cortex, where it accumulates in F-actin-rich membrane protrusions induced by polarized activation of TCR-CD3-stimulated T cells. These data are consistent with a role of Coronin-1 in T cell differentiation/activation events involving membrane dynamisms and the cortical actin cytoskeleton.
Nal
Béatrice
B
Centre d'Immunologie de Marseille-Luminy, INSERM-CNRS-Université de la Méditerranée, Case 906, 13288 Marseille Cedex 9, France.
Carroll
Patrick
P
Mohr
Elodie
E
Verthuy
Christophe
C
Da Silva
Maria-Isabel
MI
Gayet
Odile
O
Guo
Xiao-Jun
XJ
He
Hai-Tao
HT
Alcover
Andrés
A
Ferrier
Pierre
P
D000199:Actins / Q000378:metabolism; D000818:Animals; D017252:CD3 Complex / Q000276:immunology / Q000378:metabolism; D015704:CD4 Antigens / Q000276:immunology / Q000378:metabolism; D002460:Cell Line; D004622:Embryo, Mammalian / Q000276:immunology / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000235:genetics* / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation / Q000235:genetics* / Q000276:immunology; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008840:Microfilament Proteins; D011506:Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology / Q000196:embryology / Q000276:immunology; D020696:rab5 GTP-Binding Proteins / Q000276:immunology / Q000378:metabolism
D000199:Actins; D017252:CD3 Complex; D015704:CD4 Antigens; D008840:Microfilament Proteins; D011506:Proteins; C072061:coronin proteins; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D020696:rab5 GTP-Binding Proteins