title: Lymphocyte egress from thymus and peripheral lymphoid organs is dependent on S1P receptor 1.
abstract: Adaptive immunity depends on T-cell exit from the thymus and T and B cells travelling between secondary lymphoid organs to survey for antigens. After activation in lymphoid organs, T cells must again return to circulation to reach sites of infection; however, the mechanisms regulating lymphoid organ exit are unknown. An immunosuppressant drug, FTY720, inhibits lymphocyte emigration from lymphoid organs, and phosphorylated FTY720 binds and activates four of the five known sphingosine-1-phosphate (S1P) receptors. However, the role of S1P receptors in normal immune cell trafficking is unclear. Here we show that in mice whose haematopoietic cells lack a single S1P receptor (S1P1; also known as Edg1) there are no T cells in the periphery because mature T cells are unable to exit the thymus. Although B cells are present in peripheral lymphoid organs, they are severely deficient in blood and lymph. Adoptive cell transfer experiments establish an intrinsic requirement for S1P1 in T and B cells for lymphoid organ egress. Furthermore, S1P1-dependent chemotactic responsiveness is strongly upregulated in T-cell development before exit from the thymus, whereas S1P1 is downregulated during peripheral lymphocyte activation, and this is associated with retention in lymphoid organs. We find that FTY720 treatment downregulates S1P1, creating a temporary pharmacological S1P1-null state in lymphocytes, providing an explanation for the mechanism of FTY720-induced lymphocyte sequestration. These findings establish that S1P1 is essential for lymphocyte recirculation and that it regulates egress from both thymus and peripheral lymphoid organs.
authors.affiliation: Howard Hughes Medical Institute and Department of Microbiology, University of California San Francisco, 513 Parnassus Avenue, San Francisco, California 94143-0414, USA.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Matloubian
authors.forename: Charles G
authors.initials: CG
authors.lastname: Lo
authors.forename: Guy
authors.initials: G
authors.lastname: Cinamon
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Lesneski
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.forename: Volker
authors.initials: V
authors.lastname: Brinkmann
authors.forename: Maria L
authors.initials: ML
authors.lastname: Allende
authors.forename: Richard L
authors.initials: RL
authors.lastname: Proia
authors.forename: Jason G
authors.initials: JG
authors.lastname: Cyster
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002633:Chemotaxis / Q000187:drug effects; D002678:Chimera / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D000068876:Fingolimod Hydrochloride; D017353:Gene Deletion; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D008221:Lymphoid Tissue / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D008246:Lysophospholipids; D051379:Mice; D018345:Mice, Knockout; D011409:Propylene Glycols / Q000494:pharmacology; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D049312:Receptors, Lysophospholipid; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D013950:Thymus Gland / Q000166:cytology* / Q000187:drug effects / Q000276:immunology
chemical_list: D008246:Lysophospholipids; D011409:Propylene Glycols; D043562:Receptors, G-Protein-Coupled; D049312:Receptors, Lysophospholipid; C060506:sphingosine 1-phosphate; D000068876:Fingolimod Hydrochloride; D013110:Sphingosine