title: [Comparison of the effects of rhEGF with rhbFGF on the acceleration of wound healing].
abstract: OBJECTIVE
  To investigate the mechanism and the accelerating effect of rhEGF and rhbFGF on wound healing.
  
  
  METHODS
  Twelve New Zealand rabbits with 72 incised wounds on ventral side of 24 ears were randomly divided into two therapeutic groups (rhEGF of 10 ug/cm(2) and rhbFGF of 100 AU/cm(2)) and a control group (1% silver sulfadiazine cream, SD-Ag). The general conditions of the wound healing was observed grossly. Biopsies were harvested at different time points for the pathomorphological examination, the electron microscopic examination, and for assessment of integrin beta1 mRNA expression by in situ hybridization.
  
  
  RESULTS
  The expressions of integrin beta 1 mRNA in two therapeutic groups were significantly higher than that of control group. The quality of the wound healing was improved in therapeutic group with its healing time shortened when compared with that in control group (P < 0.05). There was an obvious difference in the number of fibroblasts and capillary gemmules between the therapeutic and control groups (P < 0.05).
  
  
  CONCLUSIONS
  The wound healing and quality could be improved by both rhEGF and rhbFGF, but rhbFGF seemed better to be employed during the early and middle stages of the wound repair for the growth of granulation tissue, while rhEGF should be applied at the late stage of wound repair to accelerate the re-epithelialization of the wound. Combined application of rhEGF with rhbFGF according to time effect could be more beneficial to the wound repair.
authors.affiliation: Department of Burns, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, P.R. China.
authors.forename: Bang-rong
authors.initials: BR
authors.lastname: Xing
authors.forename: Tian-zeng
authors.initials: TZ
authors.lastname: Li
authors.forename: Hui-ning
authors.initials: HN
authors.lastname: Bian
authors.forename: Shao-hai
authors.initials: SH
authors.lastname: Qi
authors.forename: Ju-lin
authors.initials: JL
authors.lastname: Xie
authors.forename: Ying-bin
authors.initials: YB
authors.lastname: Xu
authors.forename: Xiao-gen
authors.initials: XG
authors.lastname: Hu
mesh_terms: D000818:Animals; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D019012:Integrin beta1 / Q000235:genetics; D008297:Male; D008854:Microscopy, Electron; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D019012:Integrin beta1; D012333:RNA, Messenger; D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor