Comparison of genome segments 2, 7 and 10 of bluetongue viruses serotype 2 for differentiation between field isolates and the vaccine strain.
Bluetongue (BT) virus serotype 2 (BTV 2) was first confirmed in Tunisia in February 2000 and has since spread northward and westward, infecting several other countries and islands, including Corsica, where clinical disease was reported in October 2000. BT was again reported on the Island in July 2001, some six months after a vaccination campaign against BTV 2. The molecular relationship between isolates of the BTV 2 Corsican wild-type viruses from 2000 and 2001, and the attenuated BTV 2 vaccine were determined by comparing corresponding sequences of genome segments 2, 7 and 10 with each other and with already published sequences available in the genome database. Complete genetic stability was observed between the isolates of the Corsican BTV 2. There was some divergence between the nucleotide sequences of segment 10 obtained from the wild-type and vaccine virus strains. Based on these differences, primers were selected that could be used in RT-PCR to differentiate between the wild-type and the vaccine viruses.
Bréard
Emmanuel
E
UMR 1161 AFSSA-INRA-ENVA, Agence Française de Sécurité Sanitaire des Aliments - Alfort, 22 rue Pierre Curie, 94703 Maisons-Alfort, France. e.breard@afssa.fr
Sailleau
Corinne
C
Coupier
Hervé
H
Mure-Ravaud
Karine
K
Hammoumi
Saliha
S
Gicquel
Bernard
B
Hamblin
Chris
C
Dubourget
Philippe
P
Zientara
Stéphan
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001819:Bluetongue / Q000175:diagnosis* / Q000453:epidemiology / Q000517:prevention & control / Q000821:virology; D001820:Bluetongue virus / Q000235:genetics* / Q000276:immunology; D002642:Chick Embryo; D005819:Genetic Markers; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009500:Neutralization Tests / Q000662:veterinary; D012367:RNA, Viral / Q000032:analysis* / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D012703:Serotyping / Q000662:veterinary; D012756:Sheep; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines / Q000235:genetics*
D005819:Genetic Markers; D012367:RNA, Viral; D014765:Viral Vaccines