Fragmentation of amidinated peptide ions.
The collision-induced dissociation characteristics of amidinated and unmodified tryptic peptides are compared using an ion trap mass spectrometer with both electrospray ionization and matrix-assisted laser/desorption ionization (MALDI). Several fragmentation pathways in a number of tryptic peptides of various precursor charge states are found to be enhanced. The additional information conveyed by the observed fragment ions should facilitate protein identifications.
Beardsley
Richard L
RL
Department of Chemistry, Indiana University, Bloomington, Indiana 47401, USA.
Reilly
James P
JP
D000577:Amides / Q000737:chemistry*; D000595:Amino Acid Sequence; D007477:Ions / Q000737:chemistry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010446:Peptide Fragments / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000577:Amides; D007477:Ions; D010446:Peptide Fragments; D010455:Peptides