Adaptor-tagged competitive PCR: study of the mammalian nervous system.
Adaptor-tagged competitive PCR (ATAC-PCR) is an advanced form of quantitative competitive PCR, and enables high-throughput analysis of gene expression. We applied this technique to the postnatal cerebellar development. Data analysis with terms representing reported functions revealed a correlation between gene expression and functions. We also analyzed the cell death induced by extended polyglutamine, a model of a neurodegenerative disorder. We identified genes with expression patterns specific to the cell death, and evaluated their functions by in vitro transfection experiments.
Kato
Kikuya
K
Taisho Lab of Functional Genomics, Graduate School of Biological Sciences, Nara Institute of Science & Technology, Takayama, Ikoma, Nara, Japan. kkato@bs.aist-nara.ac.jp
D000818:Animals; D016923:Cell Death / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000235:genetics; D008322:Mammals; D009420:Nervous System / Q000187:drug effects / Q000254:growth & development*; D010455:Peptides / Q000494:pharmacology; D016133:Polymerase Chain Reaction / Q000379:methods*; D016324:Sequence Tagged Sites
D010455:Peptides; C097188:polyglutamine