Up-regulation of thymosin beta 4 gene expression in experimentally-induced uterine adenomyosis in mice.
Degradation of the myometrium contributes to the development of adenomyosis and disruption of the actin cytoskeleton occurs in the myometrium adjacent to the invasive endometrium. A subtractive cDNA library that contained genes up-regulated in the uterus with adenomyosis induced by pituitary grafting was constructed in mice. Thymosin beta 4 (Tb4), an actin sequestering protein, was contained in the library and was confirmed to be abundant in the adenomyotic uteri. Tb4-immunoreactivity was shown in the myometrium, with a stronger signal being observed in the area closer to the tip of endometrial tissue that is invading the myometrium. Up-regulation of Tb4 gene expression may be responsible for the disintegration of the musculature during the development of adenomyosis.
Kawahara
Ryoka
R
Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan.
Matsuda
Manabu
M
Imaoka
Tatsuhiko
T
Mori
Takao
T
D000818:Animals; D004715:Endometriosis / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D004717:Endometrium / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D015870:Gene Expression; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009215:Myometrium / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D012333:RNA, Messenger / Q000032:analysis; D013947:Thymosin / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation; D000099201:Thymosin beta(4)
D012333:RNA, Messenger; D013947:Thymosin; D000099201:Thymosin beta(4)