Allicin (from garlic) induces caspase-mediated apoptosis in cancer cells.
Garlic (Allium sativum) has been used for centuries for treating various ailments, and its consumption is said to reduce cancer risk and its extracts and components effectively block experimentally induced tumors. Allicin, the major component present in freshly crushed garlic, is one of the most biologically active compounds of garlic. We found that allicin inhibited the growth of cancer cells of murine and human origin. Allicin induced the formation of apoptotic bodies, nuclear condensation and a typical DNA ladder in cancer cells. Furthermore, activation of caspases-3, -8 and -9 and cleavage of poly(ADP-ribose) polymerase were induced by allicin. The present results demonstrating allicin-induced apoptosis of cancer cells are novel since allicin has not been shown to induce apoptosis previously. Our results also provide a mechanistic basis for the antiproliferative effects of allicin and partly account for the chemopreventive action of garlic extracts reported by earlier workers.
Oommen
Suby
S
Division of Cancer Biology, Rajiv Gandhi Centre for Biotechnology, Poojappura, Thiruvananthapuram-695014, Kerala, India.
Anto
Ruby John
RJ
Srinivas
Gopal
G
Karunagaran
Devarajan
D
D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D020169:Caspases / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004220:Disulfides; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D005737:Garlic; D006801:Humans; D009369:Neoplasms / Q000201:enzymology* / Q000473:pathology* / Q000517:prevention & control; D013441:Sulfinic Acids / Q000494:pharmacology* / Q000627:therapeutic use
D000970:Antineoplastic Agents; D004220:Disulfides; D013441:Sulfinic Acids; C006452:allicin; D020169:Caspases