Quantitative analysis of triacylglycerol regioisomers in fats and oils using reversed-phase high-performance liquid chromatography and atmospheric pressure chemical ionization mass spectrometry.
Positional distribution of fatty acyl chains of triacylglycerols (TGs) in vegetable oils and fats (palm oil, cocoa butter) and animal fats (beef, pork and chicken fats) was examined by reversed-phase high-performance liquid chromatography (RP-HPLC) coupled to atmospheric pressure chemical ionization using a quadrupole mass spectrometer. Quantification of regioisomers was achieved for TGs containing two different fatty acyl chains (palmitic (P), stearic (S), oleic (O), and/or linoleic (L)). For seven pairs of 'AAB/ABA'-type TGs, namely PPS/PSP, PPO/POP, SSO/SOS, POO/OPO, SOO/OSO, PPL/PLP and LLS/LSL, calibration curves were established on the basis of the difference in relative abundances of the fragment ions produced by preferred losses of the fatty acid from the 1/3-position compared to the 2-position. In practice the positional isomers AAB and ABA yield mass spectra showing a significant difference in relative abundance ratios of the ions AA(+) to AB(+). Statistical analysis of the validation data obtained from analysis of TG standards and spiked oils showed that, under repeatability conditions, least-squares regression can be used to establish calibration curves for all pairs. The regression models show linear behavior that allow the determination of the proportion of each regioisomer in an AAB/ABA pair, within a working range from 10 to 1000 microg/mL and a 95% confidence interval of +/-3% for three replicates.
Fauconnot
Laëtitia
L
Nestlé Research Center, Nestec Ltd. Vers-chez-les-Blanc, P.O. Box 44, CH-1000 Lausanne 26, Switzerland. laetitia.fauconnot@rdls.nestle.com
Hau
Jörg
J
Aeschlimann
Jean-Marc
JM
Fay
Laurent-Bernard
LB
Dionisi
Fabiola
F
D000818:Animals; D001274:Atmospheric Pressure; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005223:Fats / Q000737:chemistry*; D007536:Isomerism; D013058:Mass Spectrometry / Q000379:methods*; D008460:Meat; D009821:Oils / Q000737:chemistry*; D012015:Reference Standards; D014280:Triglycerides / Q000032:analysis* / Q000737:chemistry*
D005223:Fats; D009821:Oils; D014280:Triglycerides