title: The main external alternative NAD(P)H dehydrogenase of Neurospora crassa mitochondria.
abstract: A DNA sequence homologous to non-proton-pumping NADH dehydrogenase genes was found in the genome of Neurospora crassa encoding a polypeptide of 577 amino acid residues, molecular mass of 64,656 Da, with a putative transmembrane domain. Analysis of fungal mitochondria fractionated with digitonin indicates that the protein is located at the outer face of the inner membrane of the organelle (external enzyme). The corresponding gene was inactivated by the generation of repeat-induced point mutations. Mitochondria from the resulting null-mutant nde2 are highly deficient in the oxidation of cytosolic NADH and NADPH. A triple mutant nde1/nde2/ndi1, lacking mitochondrial alternative NAD(P)H dehydrogenases, was obtained, indicating that these proteins are not essential in N. crassa. However, crosses between the nde2 mutant strain and complex I-deficient mutants yielded no viable double mutants. Transcription of the nde-2 gene, as well as of ndi-1 (internal enzyme), is repressed in the late exponential phase of fungal growth.
authors.affiliation: Instituto de Biologia Molecular e Celular, Rua do Campo Alegre 823, 4150-180 Oporto, Portugal.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Carneiro
authors.forename: Margarida
authors.initials: M
authors.lastname: Duarte
authors.forename: Arnaldo
authors.initials: A
authors.lastname: Videira
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D015966:Gene Expression Regulation, Fungal; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism; D008969:Molecular Sequence Data; D009252:NADPH Dehydrogenase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D009492:Neurospora crassa / Q000201:enzymology* / Q000254:growth & development; D010101:Oxygen Consumption
chemical_list: D009252:NADPH Dehydrogenase