title: Phage display of random peptide libraries: applications, limits, and potential.
abstract: The identification of ligands from large biological libraries by phage display has now been used for almost 15 years. Most of the successful reports on high-affinity ligand identification originated from work with different antibody libraries. In contrast, the progress of applying phage display to random peptide libraries was relatively slow. However, in the last few years several improvements have led to an increasing number of published peptide ligands identified by phage display from such libraries and which exhibited good biological activity and high affinity. This review summarizes the current state and the technical progress of the application of random peptide libraries using filamentous phage for ligand identification.
authors.affiliation: Institute of Biochemistry and Biotechnology, Technical University of Braunschweig, Braunschweig, Germany. msz@winet.de
authors.forename: Michael
authors.initials: M
authors.lastname: Szardenings
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry; D017104:Bacteriophage M13 / Q000378:metabolism; D001435:Bacteriophages / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015723:Gene Library; D006801:Humans; D008024:Ligands; D008957:Models, Genetic; D019151:Peptide Library; D013997:Time Factors
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D004791:Enzyme Inhibitors; D008024:Ligands; D019151:Peptide Library