[Gene expression of toll-like receptors in the liver, lungs and spleen in mice after endotoxin challenge].
OBJECTIVE
To investigate the gene expression of some lipopolysaccharide (LPS) receptors after LPS stimulation.


METHODS
The total RNA from normal and LPS-challenged mice was extracted by Trizol reagent and the gene expression of Toll-like receptor 2 (TLR2), TLR4, CD(14), LPS-binding protein (LBP) and tumor necrosis factor-alpha(TNF-alpha) were measured by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR).


RESULTS
The gene of TLR2 was expressed in normal lungs and spleen tissues, and TLR2, CD(14), LBP in liver. After challenged by LPS, the expressions of TLR2, TLR4, and TNF-alpha in lungs, TLR2, CD(14), LBP and TNF-alpha in liver, TLR2, TLR4, CD(14), and TNF-alpha in spleen were increased at 1, 3, and 5 hours.


CONCLUSIONS
LPS might alert the ability against pathogen-associated molecules by inducing or enhancing the expression of genes that involved in the LPS signal transduction.
Wan
Xing
X
Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, Guangdong, China.
Wang
Pei-Xun
PX
Zhou
Lian
L
Xiang
Qun
Q
D000209:Acute-Phase Proteins; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice; D020559:Monomeric GTP-Binding Proteins / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000378:metabolism; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D020931:ran GTP-Binding Protein; D000099231:Lipopolysaccharide-Binding Protein
D000209:Acute-Phase Proteins; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D020559:Monomeric GTP-Binding Proteins; D011956:Receptors, Cell Surface; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors; D014409:Tumor Necrosis Factor-alpha; D020931:ran GTP-Binding Protein; D000099231:Lipopolysaccharide-Binding Protein; C102460:Ran protein, mouse