title: Effects of methanol extract of Cirsium japonicum var. ussuriense and its principle, hispidulin-7-O-neohesperidoside on hepatic alcohol-metabolizing enzymes and lipid peroxidation in ethanol-treated rats.
abstract: Effects of the methanol extract of Cirsium japonicum var. ussuriense and hispidulin 7-O-neohesperidoside isolated from the plant on hepatic alcohol-metabolizing enzymes and lipid peroxidation were studied in rats treated with ethanol. Rats treated with 10% alcohol solution for 6 weeks were orally administered with 250 or 500 mg of methanol extract or 10 or 20 mg of hispidulin 7-O-neohesperidoside per kg body weight daily during the last week of ethanol treatment. The administration of the methanol extract of herbal plant and hispidulin 7-O-neohesperidoside in ethanol-treated rats significantly enhanced the activities of hepatic alcohol dehydrogenase, microsomal ethanol-oxidizing system and aldehyde dehydrogenase in a dose-dependent manner. The extract and the compound decreased hepatic lipid peroxidation along with an increase in hepatic content of reduced glutathione. The methanol extract and hispidulin 7-O-neohesperidoside of C. japonicum var. ussuriense also increased the activity of glutathione reductase, but had no effect on gamma-glutamylcysteine synthase. The results suggest that C. japonicum var. ussuriense may alleviate alcoholic toxicity by enhancing ethanol oxidation as well as inhibiting lipid peroxidation, and hispidulin 7-O-neohesperidoside is one of the active substances responsible for the protective effects of this plant.
authors.affiliation: Department of Oriental Medicine Resources, Sunchon National University, Suncheon, Jeonnam, Republic of Korea. flavonoid@empal.com
authors.forename: Jong Cheol
authors.initials: JC
authors.lastname: Park
authors.forename: Jong Moon
authors.initials: JM
authors.lastname: Hur
authors.forename: Ju Gwon
authors.initials: JG
authors.lastname: Park
authors.forename: Sang Cheol
authors.initials: SC
authors.lastname: Kim
authors.forename: Jeong Ro
authors.initials: JR
authors.lastname: Park
authors.forename: Seong Hee
authors.initials: SH
authors.lastname: Choi
authors.forename: Jong Won
authors.initials: JW
authors.lastname: Choi
mesh_terms: D000284:Administration, Oral; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology / Q000209:etiology / Q000517:prevention & control*; D031180:Cirsium; D004151:Dipeptides / Q000187:drug effects; D000431:Ethanol; D047309:Flavones; D005419:Flavonoids / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D005980:Glutathione Reductase / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects*; D008930:Mitochondria, Liver / Q000187:drug effects; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D020011:Protective Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D017392:Thiobarbituric Acid Reactive Substances
chemical_list: D004151:Dipeptides; D047309:Flavones; D005419:Flavonoids; D010936:Plant Extracts; D020011:Protective Agents; D017392:Thiobarbituric Acid Reactive Substances; D000431:Ethanol; D005980:Glutathione Reductase; C017341:gamma-glutamylcysteine; C055957:hispidulin