title: Microglia-derived glial cell line-derived neurotrophic factor could protect Sprague-Dawley rat astrocyte from in vitro ischemia-induced damage.
abstract: The interrelationship between microglia and astrocytes in cerebral ischemia was determined in vitro by adding in vitro ischemia-induced supernatant from microglia into astrocytes under the same conditions (glucose-, oxygen- and serum-free). The involvement of glial cell line-derived neurotrophic factor (GDNF) was further investigated by immunoblocking assay and Western blot analysis. Results showed that microglia-derived supernatant protected against in vitro ischemia-induced damage of astrocytes and this protection was pre-blocked by anti-GDNF but not normal rabbit serum. In addition, in vitro ischemia appeared to induce the expression of GDNF in microglia. These results indicate that microglia-derived protection on astrocytes during in vitro ischemia is GDNF-dependent.
authors.affiliation: Department of Life Sciences, National Chung Hsing University, Taichung 40227, Taiwan.
authors.forename: Gilbert A
authors.initials: GA
authors.lastname: Lee
authors.forename: Chi-Hsin
authors.initials: CH
authors.lastname: Lin
authors.forename: Hang-Hsiang
authors.initials: HH
authors.lastname: Jiang
authors.forename: Hsin-Ju
authors.initials: HJ
authors.lastname: Chao
authors.forename: Chieh-Liang
authors.initials: CL
authors.lastname: Wu
authors.forename: Chi-Mei
authors.initials: CM
authors.lastname: Hsueh
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects*; D015153:Blotting, Western; D002478:Cells, Cultured; D051100:Glial Cell Line-Derived Neurotrophic Factor; D066298:In Vitro Techniques; D007511:Ischemia / Q000188:drug therapy*; D017628:Microglia / Q000502:physiology*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D018696:Neuroprotective Agents