Effect of antisense oligodeoxynucleotide of telomerase RNA on telomerase activity and cell apoptosis in human colon cancer.
OBJECTIVE
To explore the effect of antisense oligodeoxynucleotide (As-ODN) of telomerase RNA on telomerase activity and cell apoptosis in human colon cancer.


METHODS
As-ODN was transfected into SW480 cells by liposomal transfection reagent. Telomerase activity of SW480 cells was examined by telomeric repeat amplification protocol (TRAP) and enzyme-linked immunosorbent assay (ELISA). Apoptosis was analyzed by morphology and flow cytometry.


RESULTS
The telomerase activity in SW480 cells transfected with 1.0 micromol/L of As-ODN for 2-5 days, was significantly decreased in a time-dependent manner, and the cells underwent apoptosis. The missense ODN (Ms-ODN) and the control group transfected with SW480 cells did not show these changes.


CONCLUSIONS
As-ODN can specifically inhibit the telomerase activity of SW480 cells and induce apoptosis.
Jiang
Ying-An
YA
Department of Gastroenterology, Renming Hospital of Wuhan University, Hubei Province, China.
Luo
He-Sheng
HS
Fan
Li-Fang
LF
Jiang
Chong-Qing
CQ
Chen
Wei-Jin
WJ
D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000201:enzymology / Q000503:physiopathology*; D006801:Humans; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D012313:RNA / Q000235:genetics*; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism*
D020319:Oligodeoxyribonucleotides, Antisense; C422952:telomerase RNA; D012313:RNA; D019098:Telomerase