title: [Relationship between programmed cell death mechanisms and neuronal necrosis induced by seizures].
abstract: OBJECTIVE
  To clarify if programmed cell death mechanisms induced by seizures take part in the necrotic process of neurons.
  
  
  METHODS
  Seizure was induced by pilocarpine (P) in Sprague-Dawley adult rats which were allowed to recover for 24 or 72 hours before perfusion-fixation. Neuronal death was assessed by light microscopy with the hematoxylin-eosin (HE) staining and with in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL). Bax and Bcl-2 protein expression were examined by histochemistry.
  
  
  RESULTS
  Twenty-four and 72 hours after seizures, neuronal death in hippocampus CA1 region was morphologically necrotic. TUNEL-positive and morphologically necrotic cells increased in the hippocampal CA1 region at 72 hours after seizures, there was significant difference compared with controls (P < 0.001). Bax expression was also increased in the hippocampal CA1 region at 72 hours after seizures (P < 0.001), but Bcl-2 expression did not increase, while Bcl-2/Bax ratio decreased.
  
  
  CONCLUSIONS
  Seizures induced late-onset neuronal necrosis was accompanied by programmed cell death mechanisms.
authors.affiliation: Department of Pediatrics, The Hospital Affiliated to The College of Medicine, Yanbian University, Yanji 133000, China.
authors.forename: Ren-zhe
authors.initials: RZ
authors.lastname: An
authors.forename: Yong-ri
authors.initials: YR
authors.lastname: Yin
authors.forename: Chun-ji
authors.initials: CJ
authors.lastname: Jin
authors.forename: Zheng
authors.initials: Z
authors.lastname: Jin
authors.forename: Gen-huan
authors.initials: GH
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D006624:Hippocampus / Q000737:chemistry / Q000473:pathology*; D020287:In Situ Nick-End Labeling; D008297:Male; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced / Q000503:physiopathology*; D051028:bcl-2-Associated X Protein
chemical_list: C494828:Bax protein, rat; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein