title: [A new membrane-permeable peptide from human].
abstract: OBJECTIVE
  To study the translocation ability of a new protein domain (Circadian locomotor output cycles kaput protein's DNA-binding peptide, hCLOCK's DNA_BIND) from human through the membrane.
  
  
  METHODS
  hCLOCK's DNA_BIND, which was chemically synthesized and labeled with FITC at N-terminal, was incubated with the vascular endothelial cell (ECV-304) and primary culture neuroglial cells in the culture medium, the distribution of it was observed on a fluorescence microscope.
  
  
  RESULTS
  hCLOCK's DNA_BIND has the ability to translocate through the cell membrane and accumulate in the nucleus. The quantification of cellular uptake of it increases with the increase of incubation time and concentration. However, the peptide was internalized at 4 degrees C as efficiently as that at 37 degrees C.
  
  
  CONCLUSIONS
  It is promising to provide a new safe carrier for the intracellular treatment.
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.forename: Chun-lei
authors.initials: CL
authors.lastname: Yang
authors.forename: Jing
authors.initials: J
authors.lastname: Xiao
authors.forename: Hui-ling
authors.initials: HL
authors.lastname: Guo
authors.forename: Ying-hui
authors.initials: YH
authors.lastname: Liu
authors.forename: Zheng-rong
authors.initials: ZR
authors.lastname: Wang
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D005456:Fluorescent Dyes; D006801:Humans; D008856:Microscopy, Fluorescence; D009457:Neuroglia / Q000166:cytology* / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D005456:Fluorescent Dyes; D010455:Peptides