title: Chromosome termini of the monocot plant Othocallis siberica are maintained by telomerase, which specifically synthesises vertebrate-type telomere sequences.
abstract: Lack of Arabidopsis-type T3AG3 telomere sequences has recently been reported for the majority of investigated taxa of the monocot order Asparagales. In order to investigate this phenomenon in more detail, we conducted extensive cytogenetic and molecular analyses of the telomeres in Othocallis siberica, a member of this order. Terminal restriction fragment analysis together with Bal31 exonuclease assay showed that chromosome termini in O. siberica are formed by long stretches (more than 10 kbp) of vertebrate-type T2AG3 repeats. In addition, telomerase activity specifically synthesising (T2AG3)n sequence was detected in O. siberica protein extracts by telomerase repeat amplification protocol (TRAP). Fluorescence in situ hybridisation (FISH) revealed the presence of the vertebrate-type T2AG3 telomere sequences at all chromosome termini and at a few additional regions of O. siberica chromosomes, whereas Arabidopsis-type T3AG3 DNA and peptide nucleic acid (PNA) probes did not hybridise to chromosomes of Othocallis, except for polymorphic blocks in chromosomes 2 (interstitial) and 4 (terminal). These interstitial/terminal regions are apparently composed of large blocks of (T2AG3)n and (T3AG3)n DNA and represent a unique example of interspersion of two types of telomeric repeats within one genome. This may be a reflection of the recent evolutionary switch from Arabidopsis- to vertebrate-type telomeric repeats in this plant group.
authors.affiliation: Department of Higher Plant Systematics and Evolution, Institute of Botany, University of Vienna, Rennweg 14, A-1030 Vienna, Austria. hanna.weiss@univie.ac.at
authors.forename: Hanna
authors.initials: H
authors.lastname: Weiss-Schneeweiss
authors.forename: Karel
authors.initials: K
authors.lastname: Riha
authors.forename: Chang Gee
authors.initials: CG
authors.lastname: Jang
authors.forename: Jasna
authors.initials: J
authors.lastname: Puizina
authors.forename: Harry
authors.initials: H
authors.lastname: Scherthan
authors.forename: Dieter
authors.initials: D
authors.lastname: Schweizer
mesh_terms: D001483:Base Sequence; D001937:Brassica / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods; D032461:Chromosomes, Plant / Q000235:genetics*; D020732:Cytogenetic Analysis / Q000379:methods; D016172:DNA Fingerprinting / Q000379:methods; D004706:Endodeoxyribonucleases / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D019668:Liliaceae / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism; D016615:Telomere / Q000201:enzymology / Q000235:genetics*
chemical_list: D019098:Telomerase; D004706:Endodeoxyribonucleases; C038407:exonuclease Bal 31