title: Monocyte cytokine secretion in patients with pulmonary tuberculosis differs from that of healthy infected subjects and correlates with clinical manifestations.
abstract: Cell-mediated immunity, leading to Mycobacterium tuberculosis (Mtb)-constraining granuloma formation, is the major component of host defense against tuberculosis and is regulated by the balance of cytokines secreted mostly by mononuclear phagocytes and lymphocytes. To better understand the role of monocytes in the regulation of the immune response against pulmonary tuberculosis, we examined IL-10, IL-12 and TNF-alpha release by monocytes from healthy purified protein derivative (PPD) reactors and pulmonary tuberculosis patients with or without systemic reactions (e.g., fever, weight loss, asthenia). Our study shows that, probably as a result of in vivo priming by circulating antigens, monocytes from patients, especially those with systemic manifestations, have a biased ex vivo cytokine secretion, with high IL-10 and TNF-alpha but low IL-12, in contrast with PPD reactors. Higher spontaneous IL-10 and TNF-alpha release persisted when monocytes were co-cultured with autologous lymphocytes. Challenge of patients' monocytes with a virulent Mtb strain led to a further enhancement of IL-10 and TNF-alpha, but not of IL-12. When lymphocytes were added to these cultures, IL-10 and TNF-alpha elevation persisted and, in the patients with a systemic reaction, both IL-12 and IFN-gamma were significantly reduced compared to PPD reactors. Intragroup comparisons revealed that in the patients with systemic reactions, the lymphocyte-monocyte interaction resulted in a positive feedback for IL-10 secretion, while in the patients without systemic reaction and PPD reactors, the feedback was positive for IL-12 secretion. Thus, in tuberculosis, there appears to exist a relationship between the immunological findings and the distinct clinical manifestations.
authors.affiliation: Laboratório de Investigação Médica, Unidade 56, Departmento de Dermatologia, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Claudia Barreto
authors.initials: CB
authors.lastname: Pereira
authors.forename: Moises
authors.initials: M
authors.lastname: Palaci
authors.forename: Olavo H M
authors.initials: OH
authors.lastname: Leite
authors.forename: Alberto J S
authors.initials: AJ
authors.lastname: Duarte
authors.forename: Gil
authors.initials: G
authors.lastname: Benard
mesh_terms: D000293:Adolescent; D000328:Adult; D001247:Asthenia / Q000473:pathology; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D005334:Fever / Q000473:pathology; D006801:Humans; D016753:Interleukin-10 / Q000032:analysis / Q000096:biosynthesis; D018664:Interleukin-12 / Q000032:analysis; D008214:Lymphocytes; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000097:blood / Q000276:immunology* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000096:biosynthesis; D015431:Weight Loss
chemical_list: D016207:Cytokines; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D018664:Interleukin-12