Cloning and characterization of human synaptotagmin 10 gene.
Synaptotagmin (Syt) is a membrane protein family of secretory vesicles, abundant in neural and some endocrine cells. All the members of this family contain one transmembrane region and two conserved C2 domains. Here we reported a new human Syt 10 gene isolated when screening a human fetal brain cDNA library. This cDNA clone is 3287 bp and contains an open reading frame from 299 to 1870 encoding a putative protein of 523 amino acids. It shares 94.6 and 94.8% homology to rat Syt 10 and mouse Syt 10 at protein level, respectively. RT-PCR result showed that it is expressed only in pancreas, lung and kidney.
Zhao
Enpeng
E
State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai 200433, People's Republic of China.
Li
Yao
Y
Fu
Xuping
X
Zeng
Li
L
Zeng
Huawu
H
Jin
Wei
W
Chen
Jinzhong
J
Yin
Gang
G
Qian
Ji
J
Ying
Kang
K
Xie
Yi
Y
Zhao
Robert Chunhua
RC
Mao
Yumin
Y
D000595:Amino Acid Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D006801:Humans; D007668:Kidney / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics*; D010179:Pancreas / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D050857:Synaptotagmins
D002135:Calcium-Binding Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C488378:SYT10 protein, human; D050857:Synaptotagmins