title: MG-63 human osteosarcoma cells grown in monolayer and as three-dimensional tumor spheroids present a different metabolic profile: a (1)H NMR study.
abstract: High resolution proton nuclear magnetic resonance ((1)H NMR) spectroscopy was used to determine if the same cell line (MG-63 human osteosarcoma cells) grown in monolayer or as small (about 50-80 microm in diameter), three-dimensional tumor spheroids with no hypoxic center has different metabolic characteristics. Consequently, the (1)H NMR spectra were obtained from both types of cultures and then compared. The results indicate that the type of cellular spatial array determines specific changes in MG-63 cells. In particular, small but significant differences in lactate and alanine indicating a perturbation in energy metabolism were observed in the two cell models. In addition, although variations in CH(2) and CH(3) groups were also seen, it is not possible at this time to establish if lipid metabolism is truly different in cells and spheroids.
authors.affiliation: Dipartimento di Tecnologie e Salute, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. santini@iss.it
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Santini
authors.forename: Gabriella
authors.initials: G
authors.lastname: Rainaldi
authors.forename: Rocco
authors.initials: R
authors.lastname: Romano
authors.forename: Antonella
authors.initials: A
authors.lastname: Ferrante
authors.forename: Stefania
authors.initials: S
authors.lastname: Clemente
authors.forename: Andrea
authors.initials: A
authors.lastname: Motta
authors.forename: Pietro Luigi
authors.initials: PL
authors.lastname: Indovina
mesh_terms: D000465:Algorithms; D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D018929:Cell Culture Techniques / Q000379:methods; D002453:Cell Cycle; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008855:Microscopy, Electron, Scanning; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D014407:Tumor Cells, Cultured