title: Characterization of the protein processing and secretion pathways in a comprehensive set of expressed sequence tags from Trichoderma reesei.
abstract: Trichoderma reesei is a filamentous fungus widely used as an efficient protein producer and known to secrete large quantities of biomass degrading enzymes. Much work has been done aimed at improving the secretion efficiency of this fungus. It is generally accepted that the major bottlenecks in secretion are protein folding and ornamentation steps in this pathway. In an attempt to identify genes involved in these steps, the 5' ends of 21888 cDNA clones were sequenced from which a unique set of over 5000 were also 3' sequenced. Using annotation tools Gene Ontology terms were assigned to 2732 of the sequences. Homologs to the majority of Aspergillus niger's Srg genes as well as a number of homologs to genes involved in protein folding and ornamentation pathways were identified.
authors.affiliation: Fungal Genomics Laboratory, North Carolina State University, Suite 1200, 840 Main Campus Drive, Raleigh, NC 27606, USA.
authors.forename: S E
authors.initials: SE
authors.lastname: Diener
authors.forename: N
authors.initials: N
authors.lastname: Dunn-Coleman
authors.forename: P
authors.initials: P
authors.lastname: Foreman
authors.forename: T D
authors.initials: TD
authors.lastname: Houfek
authors.forename: P J M
authors.initials: PJ
authors.lastname: Teunissen
authors.forename: P
authors.initials: P
authors.lastname: van Solingen
authors.forename: L
authors.initials: L
authors.lastname: Dankmeyer
authors.forename: T K
authors.initials: TK
authors.lastname: Mitchell
authors.forename: M
authors.initials: M
authors.lastname: Ward
authors.forename: R A
authors.initials: RA
authors.lastname: Dean
mesh_terms: D019295:Computational Biology; D020224:Expressed Sequence Tags; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015723:Gene Library; D011499:Protein Processing, Post-Translational; D021381:Protein Transport; D017422:Sequence Analysis, DNA; D014242:Trichoderma / Q000235:genetics*
chemical_list: D005656:Fungal Proteins