[Effects of low frequency pulsed magnetic field on the proliferation and differentiation of HepG2 cells].
OBJECTIVE
To study the effects of low frequency pulsed magnetic field on the proliferation and differentiation of HepG2 cells.


METHODS
3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) colorimetry method and ELISA assay of alpha-fetoprotein (AFP) were used to determine the cell proliferation and differentiation after the cells were exposed to pulsed magnetic fields with different frequency but the same field intensity.


RESULTS
There were no significant differences in cell proliferation between sham and treated groups exposed to the field of 80 Hz, 1.55 mT for 1, 4, 8, 12, 24 h (P > 0.05). There were also no significant differences in cell proliferation and AFP secretion between sham and treated groups exposed to 16 Hz, 1.55 mT pulsed magnetic fields for 1, 4, 8, 24 h (P > 0.05).


CONCLUSIONS
There were no "window effects" found in HepG2 cells proliferation or AFP secretion at 16 Hz and 80 Hz pulsed magnetic fields.
Zheng
Tao
T
Bioelectromagnetic Lab, Institute of Electrical Engineering, Chinese Academy of Sciences, Beijing 100080, China.
Yang
Wei
W
Song
Tao
T
Huo
Xiao-lin
XL
D002454:Cell Differentiation / Q000528:radiation effects*; D002455:Cell Division / Q000528:radiation effects; D045744:Cell Line, Tumor / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D004574:Electromagnetic Fields; D006801:Humans; D000509:alpha-Fetoproteins / Q000032:analysis
D000509:alpha-Fetoproteins