Inhibitory effects of manassantin A and B isolated from the roots of Saururus chinensis on PMA-induced ICAM-1 expression.
Cell adhesion inhibitors were isolated from the methanol extract of Saururus chinensis roots by bioactivity-guided fractionation. The active compounds were identified as manassantin A ( 1) and B ( 2), dineolignan compounds. Compounds 1 and 2 inhibited PMA-induced ICAM-1/LFA-1-mediated homotypic aggregation of the HL-60 cells without cytotoxicity with MIC values of 1.0 and 5.5 nM, respectively. Even though 1 and 2 did not affect the adhesion of ICAM-1 to LFA-1, these compounds inhibited PMA-induced ICAM-1 expression in HL-60 cells in a dose-dependent fashion. These results suggest that 1 and 2 inhibit cell aggregation through down-regulation of ICAM-1 expression.
Rho
Mun-Chual
MC
Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology, Taejon, Korea.
Kwon
Oh Eok
OE
Kim
Koanhoi
K
Lee
Seung Woong
SW
Chung
Mi Yeon
MY
Kim
Young Ho
YH
Hayashi
Masahiko
M
Lee
Hyun Sun
HS
Kim
Young-Kook
YK
D002449:Cell Aggregation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005663:Furans / Q000008:administration & dosage / Q000494:pharmacology*; D018922:HL-60 Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000187:drug effects* / Q000378:metabolism; D017705:Lignans; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D018517:Plant Roots; D032134:Saururaceae; D013755:Tetradecanoylphorbol Acetate
D005663:Furans; D017705:Lignans; D010936:Plant Extracts; C482885:manassantin B; D018799:Intercellular Adhesion Molecule-1; C054895:manassantin A; D013755:Tetradecanoylphorbol Acetate