title: Development and validation of a reversed-phase liquid chromatographic method for analysis of D4T (Stavudine) in rat plasma.
abstract: An improved and simplified high-performance liquid chromatographic (HPLC) method at UV detection 265 nm is presented for the determination of d4T in rat plasma. The mobile phase consists of methanol-distilled water-acetic acid in the 23:77:0.2 (v/v) ratio. Quantification is achieved by the peak-area ratio method with reference to the internal standard. This paper presents linearity, accuracy, precision, limit of quantification and limit of detection, specificity-selectivity and sample stability data. Based on the intra and inter-day validation, all coefficients of variation (CV) were found less than 15%. The assay is sufficiently rapid and sensitive and was applied in a pharmacokinetic study in rats.
authors.affiliation: Department of Analytical Chemistry, HPLC and Pharmacokinetics Laboratory, National Center for Scientific Research, PO Box 6412/6414, Havana, Cuba. contreras@diramic.cneuro.edu.cu
authors.forename: J
authors.initials: J
authors.lastname: Contreras
authors.forename: H Ma
authors.initials: HM
authors.lastname: González
authors.forename: R
authors.initials: R
authors.lastname: Menéndez
authors.forename: M
authors.initials: M
authors.lastname: López
mesh_terms: D000818:Animals; D019380:Anti-HIV Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018119:Stavudine / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D019380:Anti-HIV Agents; D018119:Stavudine