Retention of Cr(III) by high-performance chelation ion chromatography interfaced to inductively-coupled plasma mass spectrometric detection with collision cell.
High-performance chelation ion chromatography (HPCIC) was employed to retain cationic Cr(III) on an anion-exchange column and hence allow the separation of the two most prevalent forms of chromium, Cr(II) and Cr(VI). A mobile phase of nitric acid was utilized at pH = 1.5; additionally, 2,6-pyridinedicarboxylic acid was used at a concentration of 6 mM. Additives with different structural characteristics were used in an effort to elucidate retention mechanisms. Inductively-coupled plasma mass spectrometry was used for chromium detection. A collision cell was utilized to reduce chloride-based polyatomic ions that may interfere with the detection of Cr(III), and a detection limit study yielded levels in the low part-per-billion range. The newly developed method was applied to the chromatographic analysis of samples of an incubation medium containing Cr(VI) incubated with cell nuclei.
Vonderheide
Anne P
AP
Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221-0172, USA.
Meija
Juris
J
Tepperman
Katherine
K
Puga
Alvaro
A
Pinhas
Allan R
AR
States
J Christopher
JC
Caruso
Joseph A
JA
D000818:Animals; D045744:Cell Line, Tumor; D002614:Chelating Agents / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002857:Chromium / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D012680:Sensitivity and Specificity
D002614:Chelating Agents; D002857:Chromium