[Comparison of the effects of rhEGF with rhbFGF on the acceleration of wound healing].
OBJECTIVE
To investigate the mechanism and the accelerating effect of rhEGF and rhbFGF on wound healing.


METHODS
Twelve New Zealand rabbits with 72 incised wounds on ventral side of 24 ears were randomly divided into two therapeutic groups (rhEGF of 10 ug/cm(2) and rhbFGF of 100 AU/cm(2)) and a control group (1% silver sulfadiazine cream, SD-Ag). The general conditions of the wound healing was observed grossly. Biopsies were harvested at different time points for the pathomorphological examination, the electron microscopic examination, and for assessment of integrin beta1 mRNA expression by in situ hybridization.


RESULTS
The expressions of integrin beta 1 mRNA in two therapeutic groups were significantly higher than that of control group. The quality of the wound healing was improved in therapeutic group with its healing time shortened when compared with that in control group (P < 0.05). There was an obvious difference in the number of fibroblasts and capillary gemmules between the therapeutic and control groups (P < 0.05).


CONCLUSIONS
The wound healing and quality could be improved by both rhEGF and rhbFGF, but rhbFGF seemed better to be employed during the early and middle stages of the wound repair for the growth of granulation tissue, while rhEGF should be applied at the late stage of wound repair to accelerate the re-epithelialization of the wound. Combined application of rhEGF with rhbFGF according to time effect could be more beneficial to the wound repair.
Xing
Bang-rong
BR
Department of Burns, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, P.R. China.
Li
Tian-zeng
TZ
Bian
Hui-ning
HN
Qi
Shao-hai
SH
Xie
Ju-lin
JL
Xu
Ying-bin
YB
Hu
Xiao-gen
XG
D000818:Animals; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D019012:Integrin beta1 / Q000235:genetics; D008297:Male; D008854:Microscopy, Electron; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects*
D019012:Integrin beta1; D012333:RNA, Messenger; D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor