title: Regulatory light chain phosphorylation increases eccentric contraction-induced injury in skinned fast-twitch fibers.
abstract: During contraction, activation of Ca(2+)/calmodulin-dependent myosin light chain kinase (MLCK) results in phosphorylation of myosin's regulatory light chain (RLC), which potentiates force and increases speed of force development over a wide range of [Ca(2+)]. We tested the hypothesis that RLC phosphorylation by MLCK mediates the extent of eccentric contraction-induced injury as measured by force deficit in skinned fast-twitch skeletal muscle fibers. Results indicated that RLC phosphorylation in single skinned rat psoas fibers significantly increased Ca(2+) sensitivity of isometric force; isometric force from 50 +/- 16 to 59 +/- 18 kN/m(2) during maximal Ca(2+) activation; peak absolute power output from 38 +/- 15 to 48 +/- 14 nW during maximal Ca(2+) activation; and the magnitude of contraction-induced force deficit during maximal (pCa 4.5) activation from 26 +/- 9.8 to 35 +/- 9.6%. We conclude that RLC phosphorylation increases force deficits following eccentric contractions, perhaps by increasing the number of force-generating cross-bridges.
authors.affiliation: Department of Physical Medicine and Rehabilitation, DCO 46.00, One Hospital Drive, University of Missouri-Columbia, Columbia, Missouri 65212, USA. childersmk@health.missouri.edu
authors.forename: Martin K
authors.initials: MK
authors.lastname: Childers
authors.forename: Kerry S
authors.initials: KS
authors.lastname: McDonald
mesh_terms: D000818:Animals; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000502:physiology*; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism*; D018994:Myosin Light Chains / Q000378:metabolism*; D010766:Phosphorylation; D016658:Psoas Muscles / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018994:Myosin Light Chains