Differentially expressed genes in hypertensive rats developing cerebral ischemia.
The molecular events occurring after cerebral ischemia in hypertension may include de novo expression of numerous genes. Receptor genes are predominantly involved in the process of cell death, neuroprotection and reconstruction after ischemic injury. Ischemic stroke was observed in the non-genetic, non-surgical model of hypertension, the cold-induced hypertensive rat. In hypertensive rats suppression subtractive hybridization analysis was used to identify differentially expressed receptor genes in stroke-tissue compared to normal rat brain. We found 76 genes predominantly expressed in hypertensive rat stroke-tissue. These predominantly expressed genes included genes involved in energy metabolism, signal transduction/cell regulation, and replication/transcription/translation. For example, the T3 receptor alpha was predominantly expressed in stroke-tissue, indicating that regeneration of nerves in stroke tissue may be facilitated by increased T3 receptor alpha expression.
Zhu
Zhiming
Z
Department of Hypertension and Endocrinology, Daping Hospital, Third Military Medical University, Chongqing 400042, PR China. Zhuzm@yahoo.com
Zhao
Bin
B
Wang
Xianmei
X
Zhu
Shanjun
S
Zhang
Qian
Q
Xu
Youyuan
Y
Hui
Rutai
R
Tepel
Martin
M
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002545:Brain Ischemia / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D030541:Databases, Genetic; D005786:Gene Expression Regulation; D006973:Hypertension / Q000235:genetics* / Q000378:metabolism; D012313:RNA / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D011988:Receptors, Thyroid Hormone / Q000378:metabolism; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D018076:DNA, Complementary; D011988:Receptors, Thyroid Hormone; D012313:RNA