The endoproteolytic processing of alphavbeta5 integrin is involved in cytoskeleton remodelling and cell migration.
We previously showed that the post-translational cleavage of alphav subunit is essential for integrin-dependent signalling and cell adhesion. Here, we report that blocking alphav subunit cleavage by expression of alpha1-PDX, a convertase inhibitor, modified the capacity of cells to change shape, via a remodelling of the actin cytoskeleton upon cell attachment. These changes are associated with cell scattering and with a dramatic increase in cell migration to vitronectin. The alphav subunit cleavage is thus essential for integrin function and has a considerable impact on integrin-dependent events, especially those leading to cell migration.
Berthet
Virginie
V
CNRS UMR6032, Faculté de Pharmacie, 27 Bd J. Moulin, 13385 Marseille Cedex 5, France.
Rigot
Véronique
V
Nejjari
Mimoun
M
Marvaldi
Jacques
J
Luis
José
J
D000230:Adenocarcinoma; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D003599:Cytoskeleton / Q000502:physiology*; D006801:Humans; D016023:Integrins / Q000737:chemistry* / Q000378:metabolism*; D007797:Laminin / Q000494:pharmacology; D011499:Protein Processing, Post-Translational; D019030:Receptors, Vitronectin / Q000737:chemistry* / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014162:Transfection; D014407:Tumor Cells, Cultured; D019096:Vitronectin / Q000494:pharmacology
D016023:Integrins; D007797:Laminin; D019030:Receptors, Vitronectin; D019096:Vitronectin; C064660:integrin alphaVbeta5; D013755:Tetradecanoylphorbol Acetate