[Study on mitochondrial DNA damage in peripheral blood nucleate cells of the workers exposed to acrylonitrile].
OBJECTIVE
To study the potential aging effect on workers exposed to acrylonitrile (ACN).


METHODS
The deletion rates of mitochondrial DNA (mtDNA) in peripheral blood nucleate cells of 47 exposed workers and 47 non-exposed workers (as control), as well as 12 old people and 12 young people were measured with polymerase chain reaction (PCR).


RESULTS
The positive rates of mtDNA deletion in peripheral blood nucleate cells were 17.02% in the workers exposed to ACN and 25.00% in group of old people. However, the mtDNA deletion was not detected in the control group and young people.


CONCLUSIONS
ACN could induce mtDNA deletion in peripheral blood nucleate cells of the exposed workers. There may be a potential molecular effect of occupational ACN exposure on workers' aging.
Ding
Sheng
S
Department of occupational health, Jinshan hospital, Fudan university, Shanghai 200540, China.
Ma
Lai-ji
LJ
Fan
Wei
W
Zhu
Rui-juan
RJ
Ying
Qi
Q
Zhou
Yuan-ling
YL
Jin
Fu-sheng
FS
D000181:Acrylonitrile / Q000633:toxicity*; D000293:Adolescent; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000187:drug effects*; D001773:Blood Cells / Q000187:drug effects* / Q000648:ultrastructure; D004249:DNA Damage; D004272:DNA, Mitochondrial / Q000187:drug effects*; D006801:Humans; D016273:Occupational Exposure
D004272:DNA, Mitochondrial; D000181:Acrylonitrile