title: Phenotypic expression of bone-related genes in osteoblasts grown on calcium phosphate ceramics with different phase compositions.
abstract: Calcium phosphate ceramics with different hydroxyapatite (HA) and tricalcium phosphate (TCP) ratios have different chemical properties. Does the difference in phase composition affect osteoblast behavior? In this study, osteoblasts were cultured on 4 kinds of calcium phosphate ceramics, i.e. pure (HA), HT1 (HA/TCP, 70/30), HT2 (HA/TCP, 35/65), and pure TCP. Cell proliferation of SaOS-2 cells together with bone-related genes' mRNA expression and protein production in osteoblasts cultured on different calcium phosphate ceramics were detected at different time points. Data suggested that cell proliferation rate on TCP ceramics was lower than that on the other substrates tested. Generally, mRNA expressions for osteonectin and osteocalcin were similar among the four kinds of ceramics in most circumstances, whereas at six days, alkaline phosphatase mRNA expression was higher on HA and HT1 surfaces than on the other two materials. Collagen I mRNA expression was also affected by the phase composition of substrates. Osteocalcin and bone sialoprotein production in SaOS-2 cells was very similar no matter which ceramic surface the cells were grown upon. This study revealed that calcium phosphate ceramics substrate could support osteoblast growth and bone-related gene expression and its gene expression pattern explained the basis of the biocompatibility and bioactivity for calcium phosphate ceramics.
authors.affiliation: Engineering Research Center in Biomaterials, Sichuan University, Chengdu 610064, China. wangcyscu2001@yahoo.com
authors.forename: Chaoyuan
authors.initials: C
authors.lastname: Wang
authors.forename: Yourong
authors.initials: Y
authors.lastname: Duan
authors.forename: Boban
authors.initials: B
authors.lastname: Markovic
authors.forename: James
authors.initials: J
authors.lastname: Barbara
authors.forename: C Rolfe
authors.initials: CR
authors.lastname: Howlett
authors.forename: Xingdong
authors.initials: X
authors.lastname: Zhang
authors.forename: Hala
authors.initials: H
authors.lastname: Zreiqat
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000166:cytology / Q000378:metabolism*; D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D002516:Ceramics / Q000737:chemistry*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000378:metabolism*; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D002130:Calcium Phosphates; D012333:RNA, Messenger; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous