Effectiveness and limitations of uracil-DNA glycosylases in sensitive real-time PCR assays.
Pierce
Kenneth E
KE
Department of Biology MS-008, Brandeis University, Waltham, MA 02454-9110, USA. pierce@brandeis.edu
Wangh
Lawrence J
LJ
D016477:Artifacts; D045647:DNA Glycosylases / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D009687:Nuclear Proteins; D009862:Online Systems; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D051876:Sex-Determining Region Y Protein; D013696:Temperature; D014157:Transcription Factors; D051981:Uracil-DNA Glycosidase
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; D045647:DNA Glycosylases; D051981:Uracil-DNA Glycosidase