title: Polymorphous low-grade adenocarcinoma of the major salivary glands: report of three cases in an unusual location.
abstract: OBJECTIVE
  Polymorphous low-grade adenocarcinoma (PLGA) is the second most common type of malignant neoplasm in minor salivary glands. Its origin in major salivary glands is considered exceedingly rare. Herein, we present three cases of de novo PLGA arising in major salivary glands.
  
  
  RESULTS
  Three cases of PLGA were identified in a large series of primary tumours of major salivary glands. We investigated their clinicopathological profiles, including immunohistochemical features. The three patients (two men and one woman) were 51, 65, and 79 years old. The tumours were 20-30 mm large; two were in the parotid gland and one in the submandibular gland. Histologically, all the tumours had a polymorphous architectural pattern showing predominantly solid, tubular, and cribriform features and invasive growth. Papillary areas were observed focally in two tumours and an 'Indian-file' array in one. The tumour cells had a bland cytological appearance and low mitotic count. Two tumours showed perineural invasion. No preexisting pleomorphic adenoma component was identified. In all cases, tumour cells were positive for epithelial markers, S100 protein, and vimentin but negative for alpha-smooth muscle actin, muscle-specific actin, and glial fibrillary acidic protein. Proliferative activities assessed with the Ki67 labelling index were 4.3%, 7.1%, and 7.6%; no p53 overexpression was observed. Two patients had local recurrence, but none had metastasis or died of tumour.
  
  
  CONCLUSIONS
  PLGAs arising in major salivary glands and those in minor salivary glands have similar clinicopathological and immunohistochemical characteristics. It is important to recognize that PLGA can occur ab initio in the major salivary glands, although it is extremely rare.
authors.affiliation: Division of Anatomic Pathology, Mayo Clinic, Rochester, MN, USA.
authors.forename: T
authors.initials: T
authors.lastname: Nagao
authors.forename: T A
authors.initials: TA
authors.lastname: Gaffey
authors.forename: P A
authors.initials: PA
authors.lastname: Kay
authors.forename: H
authors.initials: H
authors.lastname: Minato
authors.forename: H
authors.initials: H
authors.lastname: Serizawa
authors.forename: J E
authors.initials: JE
authors.lastname: Lewis
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D000368:Aged; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010307:Parotid Neoplasms / Q000378:metabolism / Q000473:pathology*; D012189:Retrospective Studies; D013365:Submandibular Gland Neoplasms / Q000378:metabolism / Q000473:pathology*