title: [Human antibodies to cedar pollen secreted by human peripheral lymphocytes transformed by Epstein-Barr virus].
abstract: To establish immortalized human B-cells secreting antibodies to cedar pollen allergens, peripheral blood lymphocytes from 13 donors were transformed with Epstein-Barr virus. Of 5000 micro culture wells with transformed cell growth, supernatants from 88 wells were found to contain antibodies to pollen allergens. Fourteen supernatants reacted with a cedar allergen Cry j1 and 10 reacted with Cry j2. IgM class antibodies were predominant.
authors.affiliation: National Food Research Institute, Tokyo, Japan. shinmoto@affrc.go.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shinmoto
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Takahashi
authors.forename: Michie
authors.initials: M
authors.lastname: Shinmoto
mesh_terms: D000906:Antibodies / Q000097:blood*; D000921:Antibody-Producing Cells; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002472:Cell Transformation, Viral; D004854:Herpesvirus 4, Human; D006801:Humans; D011058:Pollen / Q000276:immunology*
chemical_list: D000906:Antibodies