title: Protective effect of Blue-M1 against oxidative stress on COS-1 cells.
abstract: Blue-M1 is a blue pigment formed from xylose and glycine in the Maillard reaction. Previous work revealed that Blue-M1 scavenged hydroxyl radicals, and prevented the autoxidation of linoleic acid in vitro. We investigated the protective effect of Blue-M1 for 2,2'-azobis(2-amidino-propane)dihydrochloride (AAPH)-induced toxicity in COS-1 cells. COS-1 cells were cultured in AAPH containing DMEM medium with or without Blue-M1 at 37 degrees C for 24 h. Blue-M1 decreased the AAPH-induced toxicity in COS-1 cells, and this effect was dose-dependent. Furthermore, COS-1 cells were treated with diphenyl-1-pyrenylphosphine (DPPP), as a reagent for the detection of lipid peroxide, and then were cultured in AAPH containing DMEM medium with or without Blue-M1 at 37 degrees C for 6 h. Blue-M1 prevented the AAPH-induced peroxidation of cell membrane on COS-1 cells, and this effect was also dose-dependent. These results suggest that Blue-M1 prevents the oxidative cell injury. Therefore, Blue-M1 will be an antioxidant, which protect against the oxidative stress in living systems.
authors.affiliation: Department of Agricultural Chemistry, Faculty of Agriculture, Meiji University.
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Usui
authors.forename: Satomi
authors.initials: S
authors.lastname: Shizuuchi
authors.forename: Hirohito
authors.initials: H
authors.lastname: Watanabe
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Hayase
mesh_terms: D000578:Amidines / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D019556:COS Cells / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005990:Glycerol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D015416:Maillard Reaction; D018384:Oxidative Stress; D011758:Pyrroles / Q000494:pharmacology*
chemical_list: C492601:5-((1,4-(dicarboxymethyl)-5-(2,3-dihydroxypropyl)-2-pyrrolo(3,2-b)pyrrolyl)methine)-1,4-(dicarboxymethyl)-2-(1,2,3-trihydroxypropyl)pyrrolo(3,2-b)pyrrolylium; D000578:Amidines; D000975:Antioxidants; D011758:Pyrroles; C046728:2,2'-azobis(2-amidinopropane); D005990:Glycerol