[Human antibodies to cedar pollen secreted by human peripheral lymphocytes transformed by Epstein-Barr virus].
To establish immortalized human B-cells secreting antibodies to cedar pollen allergens, peripheral blood lymphocytes from 13 donors were transformed with Epstein-Barr virus. Of 5000 micro culture wells with transformed cell growth, supernatants from 88 wells were found to contain antibodies to pollen allergens. Fourteen supernatants reacted with a cedar allergen Cry j1 and 10 reacted with Cry j2. IgM class antibodies were predominant.
Shinmoto
Hiroshi
H
National Food Research Institute, Tokyo, Japan. shinmoto@affrc.go.jp
Takahashi
Toshihiro
T
Shinmoto
Michie
M
D000906:Antibodies / Q000097:blood*; D000921:Antibody-Producing Cells; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002472:Cell Transformation, Viral; D004854:Herpesvirus 4, Human; D006801:Humans; D011058:Pollen / Q000276:immunology*
D000906:Antibodies