title: An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts.
abstract: Ultrathin sections of tissue cysts isolated from the brain of Toxoplasma gondii infected mice were submitted to two different methodologies derived from the periodic acid - Schiff's reagent (PAS) technique. The use of osmium tetroxide vapor as a developing agent of the aldehyde oxidation to reveal polysaccharides with periodic acid resulted in positive reaction in amylopectin granules in bradyzoites, as well as in the wall and matrix of the cysts, with excellent increment of the ultrastructural morphology. This technique can be used for study of T. gondii-host cell intracellular cycle, the differentiation tachyzoite-bradyzoite, and also for the formation of cysts into the host cells.
authors.affiliation: Laboratório de Ultra-estrutura Celular, Departamento de Ultra-estrutura e Biologia Celular, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, 21045-900, Brasil.
authors.forename: Erick Vaz
authors.initials: EV
authors.lastname: Guimarães
authors.forename: Laís
authors.initials: L
authors.lastname: de Carvalho
authors.forename: Helene Santos
authors.initials: HS
authors.lastname: Barbosa
mesh_terms: D000687:Amylopectin / Q000648:ultrastructure*; D000818:Animals; D001921:Brain / Q000469:parasitology*; D003560:Cysts / Q000737:chemistry / Q000469:parasitology* / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D009993:Osmium Tetroxide; D014122:Toxoplasma / Q000737:chemistry* / Q000648:ultrastructure; D014124:Toxoplasmosis, Animal / Q000469:parasitology* / Q000473:pathology
chemical_list: D000687:Amylopectin; D009993:Osmium Tetroxide