Comparison of RiboGreen and 18S rRNA quantitation for normalizing real-time RT-PCR expression analysis.
Hashimoto
Joel G
JG
Oregon Health & Science University, Portland, OR, USA.
Beadles-Bohling
Amy S
AS
Wiren
Kristine M
KM
D005456:Fluorescent Dyes / Q000737:chemistry*; D020869:Gene Expression Profiling / Q000379:methods* / Q000592:standards; D007202:Indicators and Reagents / Q000737:chemistry*; D009862:Online Systems / Q000592:standards; D012313:RNA / Q000032:analysis / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000032:analysis / Q000235:genetics*; D012015:Reference Standards; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods / Q000592:standards
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D012337:RNA, Ribosomal, 18S; D012313:RNA