Capn5 is expressed in a subset of T cells and is dispensable for development.
The Capn5 gene was inactivated by homologous recombination in ES cells that subsequently colonized the germ line of mice. The targeted mutation integrated a lacZ expression cassette into the Capn5 gene, allowing the expression of Capn5 mRNA to be examined in detail in heterozygous animals. Expression was observed in embryonic and newborn thymuses, in various epithelial tissues, and in tissues of the central nervous system. In the thymus, Capn5 was expressed mainly in relatively immature CD25(+) embryonic thymocytes. Despite the numerous expression sites of Capn5, the majority of Capn5-null mice were viable and fertile and appeared healthy. Histopathological analysis did not reveal any differences between Capn5-null and wild-type mice. There were no defects in the major T- or B-cell populations in the thymus, spleen, bone marrow, or peritoneum, nor did apoptosis appear abnormal in Capn5-null T cells. There was no evidence for the development of autoimmune disease in Capn5-null animals. However, a small proportion of homozygous null offspring from heterozygous matings were runted and most often did not survive to adulthood.
Franz
Tanna
T
Department of Developmental Immunology, Max Planck Institute for Immunobiology, D-79108 Freiburg, Germany.
Winckler
Lara
L
Boehm
Thomas
T
Dear
T Neil
TN
D000483:Alleles; D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism; D002154:Calpain / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000196:embryology / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D017353:Gene Deletion; D020869:Gene Expression Profiling; D006128:Growth / Q000502:physiology*; D006579:Heterozygote; D006720:Homozygote; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015996:Survival Rate; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology / Q000196:embryology / Q000378:metabolism
D012333:RNA, Messenger; D002154:Calpain; C403235:Capn5 protein, mouse