Transformation of NIH 3T3 cells by enhanced PAR expression.
Prostate androgen regulated (PAR) is a 1038bp novel gene located on chromosome 1 in epidermal differentiation complex. The gene is ubiquitously expressed in normal tissues and is overexpressed in most of their malignant counterparts. PAR cellular function is unknown. Here we report the effect of increased PAR expression induced by transfection of PAR cDNA on NIH3T3 cell phenotype. PAR-NIH3T3 transfectants expressing 3- to 4-fold higher PAR levels compared to controls grew faster in tissue cultures, formed colonies in soft agar, and exhibited a shortening of G1 and S phases of cell cycle and formed tumors in SCID mice. Transfection of NIH3T3 cells with increased ectopic PAR expression with a 22 mer oligonucleotide in antisense orientation with PAR mRNA abrogated their ability to form colonies in soft agar. The data presented here along with our previously reported results on DU145 cells transfected with antisense PAR cDNA suggest that PAR gene behaves like a proto-oncogene.
Platica
Micsunica
M
Department of Medicine, Mount Sinai Medical Center, New York, NY 10029, USA. mickey.platica@mssm.edu
Ivan
Elena
E
Ionescu
Alin
A
Holland
James F
JF
Mora
Gloria
G
Tindall
Donald J
DJ
Mandeli
John
J
Unger
Pamela D
PD
Platica
Ovid
O
D000818:Animals; D002455:Cell Division / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism*; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006651:Histocytochemistry; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D016513:Mice, SCID; D041681:NIH 3T3 Cells; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism; D000090063:Proto-Oncogene Mas; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
C407989:JTB protein, human; C000715470:MAS1 protein, human; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D000090063:Proto-Oncogene Mas; D011994:Recombinant Proteins