Cellular localization of Shab and Shaw potassium channels in the lobster stomatogastric ganglion.
The motor pattern generated by the 14 neurons composing the pyloric circuit in the stomatogastric ganglion (STG) of the spiny lobster, Panulirus interruptus, is organized not only by the synaptic connections between neurons, but also by the characteristic intrinsic electrophysiological properties of the individual cells. These cellular properties result from the unique complement of ion channels that each cell expresses, and the distribution of those channels in the cell membranes. We have mapped the STG expression of shab and shaw, two genes in the Shaker superfamily of potassium channel genes that encode voltage-dependent, non-inactivating channels. Using antibodies developed against peptide sequences from the two channel proteins, we explored the localization and cell-specific expression of the channels. Anti-Shab and anti-Shaw antibodies both stain all the pyloric neurons in the somata, as well as their primary neurites and branch points of large neurites, but to varying degrees between cell types. Staining was weak and irregular (Shaw) or absent (Shab) in the fine neuropil of pyloric neurons, where most synaptic interactions occur. There is a high degree of variability in the staining intensity among neurons of a single cell class. This supports Golowasch et al.'s [J Neurosci 19 (1999) RC33; Neural Comput 11 (1999) 1079] hypothesis that individual cells can have similar firing properties with varying compositions of ionic currents. Both antibodies stain the axons of the peripheral nerves as they enter foregut muscles. We conclude that both Shab and Shaw channels are appropriately localized to contribute to the noninactivating potassium current in the stomatogastric nervous system.
French
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Department of Neurobiology and Behavior, Seeley G. Mudd Hall, Cornell University, Ithaca, NY, USA. lfrench@allegheny.edu
Lanning
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Matly
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Harris-Warrick
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D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D051668:Delayed Rectifier Potassium Channels; D017952:Ganglia, Invertebrate / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D018613:Microscopy, Confocal / Q000379:methods; D009415:Nerve Net / Q000378:metabolism; D009469:Neuromuscular Junction / Q000378:metabolism; D033521:Palinuridae; D010455:Peptides / Q000276:immunology; D010525:Peripheral Nerves / Q000378:metabolism; D015221:Potassium Channels / Q000737:chemistry / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated; D011708:Pylorus / Q000294:innervation; D051669:Shab Potassium Channels; D051670:Shaw Potassium Channels; D013270:Stomach / Q000294:innervation*
D051668:Delayed Rectifier Potassium Channels; D010455:Peptides; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D051669:Shab Potassium Channels; D051670:Shaw Potassium Channels