Oxidative stress in abetalipoproteinemia patients receiving long-term vitamin E and vitamin A supplementation.
BACKGROUND
Patients with abetalipoproteinemia develop progressive ataxic neuropathy and retinopathy that are thought to be due, in part, to oxidative damage resulting from deficiencies of vitamins E and A.


OBJECTIVE
The goal was to determine the degree of oxidative stress in abetalipoproteinemia patients who had received vitamin E (100 mg/kg) and vitamin A (10 000-15 000 IU/d) since infancy.


METHODS
Ten patients aged 3-25 y were studied. Assessed were plasma carbonyl concentrations as a marker of oxidative damage to proteins; total plasma oxidizability, which was used to evaluate the susceptibility of plasma lipoproteins to oxidation; and cyclic voltammetry, which represents the overall reducing and antioxidant capacity stemming from low-molecular-weight antioxidants in plasma.


RESULTS
Concentrations of plasma carbonyls did not differ significantly between patients and control subjects ( +/- SE: 0.5670 +/- 0.031 and 0.5039 +/- 0.0134 nmol/mg protein, respectively). The lag phase of plasma oxidizability was 28.03 +/- 3.16 min in the patients and 24.0 +/- 2.79 min in healthy subjects in whom oxidizability of isolated HDL was measured (NS). Cyclic voltammetry showed a peak potential of 330 +/- 8.3 mV in all samples studied, denoting that the same antioxidants were present in the plasma of the patients and the control subjects. The anodic current of the samples, a measure of the concentration of hydrophilic low-molecular-weight antioxidants, was 5.227 +/- 0.25 and 5.38 +/- 0.20 micro A in the patients and the control subjects, respectively (NS).


CONCLUSIONS
Enhanced oxidative stress is not apparent in the plasma of abetalipoproteinemia patients receiving long-term supplementation with vitamins E and A.
Granot
Esther
E
Department of Pediatrics, Hadassah University Hospital, Jerusalem, Israel. etgranot@md.huji.ac.il
Kohen
Ron
R
D000012:Abetalipoproteinemia / Q000097:blood / Q000188:drug therapy*; D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D006801:Humans; D018384:Oxidative Stress; D012016:Reference Values; D014801:Vitamin A / Q000627:therapeutic use*; D014810:Vitamin E / Q000097:blood / Q000627:therapeutic use*; D019207:beta Carotene / Q000097:blood
D019207:beta Carotene; D014801:Vitamin A; D014810:Vitamin E