title: Changes in dietary fat intake alter plasma levels of oxidized low-density lipoprotein and lipoprotein(a).
abstract: OBJECTIVE
  To assess the effects of dietary modifications on oxidized low-density lipoprotein (LDL).
  
  
  RESULTS
  Thirty-seven healthy women were fed two diets. Both diets contained a reduced amount of total and saturated fat. In addition, one diet was low in vegetables and the other was high in vegetables, berries, and fruit. The dietary intake of total fat was 70 g per day at baseline and decreased to 56 g (low-fat, low-vegetable diet) and to 59 g (low-fat, high-vegetable diet). The saturated fat intake decreased from 28 g to 20 g and to 19 g, and the amount of polyunsaturated fat intake increased from 11 g to 13 g and to 19 g (baseline; low-fat, low-vegetable; low-fat, high-vegetable; respectively). The amount of oxidized LDL in plasma was determined as the content of oxidized phospholipid per ApoB-100 using a monoclonal antibody EO6 (OxLDL-EO6). The median plasma OxLDL-EO6 increased by 27% (P<0.01) in response to the low-fat, low-vegetable diet and 19% (P<0.01) in response to the low-fat, high-vegetable diet. Also, the Lp(a) concentration was increased by 7% (P<0.01) and 9% (P=0.01), respectively.
  
  
  CONCLUSIONS
  Alterations in the dietary fat intake resulted in increased plasma concentrations of lipoprotein(a) and OxLDL-EO6.
authors.affiliation: Department of Internal Medicine and Biocenter Oulu, University of Oulu, Finland. Marja-Leena.Silaste@oulu.fi
authors.forename: Marja-Leena
authors.initials: ML
authors.lastname: Silaste
authors.forename: Maire
authors.initials: M
authors.lastname: Rantala
authors.forename: Georg
authors.initials: G
authors.lastname: Alfthan
authors.forename: Antti
authors.initials: A
authors.lastname: Aro
authors.forename: Joseph L
authors.initials: JL
authors.lastname: Witztum
authors.forename: Y Antero
authors.initials: YA
authors.lastname: Kesäniemi
authors.forename: Sohvi
authors.initials: S
authors.lastname: Hörkkö
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000032:analysis*; D001205:Ascorbic Acid / Q000097:blood; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D002097:C-Reactive Protein / Q000032:analysis; D002338:Carotenoids / Q000097:blood; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D018752:Diet, Fat-Restricted / Q000009:adverse effects*; D004041:Dietary Fats / Q000494:pharmacology*; D005260:Female; D005638:Fruit; D006801:Humans; D017270:Lipoprotein(a) / Q000097:blood*; D008077:Lipoproteins, LDL / Q000097:blood* / Q000276:immunology; D008875:Middle Aged; D024505:Tocopherols / Q000097:blood; D014280:Triglycerides / Q000097:blood; D014675:Vegetables
chemical_list: D000975:Antioxidants; D001323:Autoantibodies; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D004041:Dietary Fats; D017270:Lipoprotein(a); D008077:Lipoproteins, LDL; D014280:Triglycerides; C099252:oxidized low density lipoprotein; D002338:Carotenoids; D002097:C-Reactive Protein; D002784:Cholesterol; D001205:Ascorbic Acid; D024505:Tocopherols