Antioxidant and neuroprotective activity of the extract from the seaweed, Halimeda incrassata (Ellis) Lamouroux, against in vitro and in vivo toxicity induced by methyl-mercury.
A growing body of evidence suggests oxidative stress as part of the toxicity mechanism of methyl-mercury (MeHg) in cell cultures and animal models and so justifies the use of natural antioxidants as therapeutic alternatives. This research examines the effect of an aqueous extract from the marine seaweed Halimeda incrassata (Hi) against the oxidative stress induced by MeHg on in vitro and in vivo models. In GT1-7 mouse hypothalamic cell cultures, the extract of Hi increased cell viability and reduced ROS production after 24-h exposure to MeHgCl. Wistar rats, acutely intoxicated with MeHgCl, had reduced levels of serum and brain thiobarbituric reactive substances when treated with the Hi extract. Similarly, animals exposed to repeated doses of MeHgCl were protected by the seaweed extract from variations in body weight, food consumption and the appearance of neurological effects. This research supports the notion that oxidative stress is directly involved in MeHg intoxication, so that natural antioxidants, particularly those in the extract of Hi, can be useful therapeutic alternatives.
Linares
Adyary Fallarero
AF
Pharmacology-Toxicology Group, Faculty of Biology, University of Havana, La Habana, Cuba.
Loikkanen
Jarkko
J
Jorge
Mancini-Filho
MF
Soria
René Barro
RB
Novoa
Alexis Vidal
AV
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D001921:Brain / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D004195:Disease Models, Animal; D007031:Hypothalamus / Q000166:cytology / Q000187:drug effects*; D008297:Male; D008767:Methylmercury Compounds / Q000633:toxicity*; D051379:Mice; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D012624:Seaweed; D017392:Thiobarbituric Acid Reactive Substances
D000975:Antioxidants; D008767:Methylmercury Compounds; D018696:Neuroprotective Agents; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances