Desulfatibacillum aliphaticivorans gen. nov., sp. nov., an n-alkane- and n-alkene-degrading, sulfate-reducing bacterium.
A novel marine sulfate-reducing bacterium, strain CV2803T, which is able to oxidize aliphatic hydrocarbons, was isolated from a hydrocarbon-polluted marine sediment (Gulf of Fos, France). The cells were rod-shaped and slightly curved, measuring 0.6x2.2-5.5 microm. Strain CV2803T stained Gram-negative and was non-motile and non-spore-forming. Optimum growth occurred in the presence of 24 g NaCl l(-1), at pH 7.5 and at a temperature between 28 and 35 degrees C. Strain CV2803T oxidized alkanes (from C13 to C18) and alkenes (from C7 to C23). The DNA G+C content was 41.4 mol%. Comparative sequence analyses of the 16S rRNA gene and dissimilatory sulfite reductase (dsrAB) gene and those of other sulfate-reducing bacteria, together with its phenotypic properties, indicated that strain CV2803T was a member of a distinct cluster that contained unnamed species. Therefore, strain CV2803T (=DSM 15576T=ATCC BAA-743T) is proposed as the type strain of a novel species in a new genus, Desulfatibacillum aliphaticivorans gen. nov., sp. nov.
Cravo-Laureau
Cristiana
C
Laboratoire de Microbiologie, IMEP CNRS UMR 6116, Faculté des Sciences et Techniques de Saint-Jérôme, case 452, 13397 Marseille cedex 20, France.
Matheron
Robert
R
Laboratoire de Microbiologie, IMEP CNRS UMR 6116, Faculté des Sciences et Techniques de Saint-Jérôme, case 452, 13397 Marseille cedex 20, France.
Cayol
Jean-Luc
JL
Laboratoire de Microbiologie IRD, IFR-BAIM case 925, Universités de Provence et de la Méditerranée, 163 avenue de Luminy, 13288 Marseille cedex 9, France.
Joulian
Catherine
C
Laboratoire de Microbiologie IRD, IFR-BAIM case 925, Universités de Provence et de la Méditerranée, 163 avenue de Luminy, 13288 Marseille cedex 9, France.
Hirschler-Réa
Agnès
A
Laboratoire de Microbiologie, IMEP CNRS UMR 6116, Faculté des Sciences et Techniques de Saint-Jérôme, case 452, 13397 Marseille cedex 20, France.
D000473:Alkanes / Q000493:pharmacokinetics; D000475:Alkenes / Q000493:pharmacokinetics; D001673:Biodegradation, Environmental; D020564:Deltaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D008658:Inactivation, Metabolic; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010802:Phylogeny; D012623:Seawater / Q000382:microbiology; D013431:Sulfates / Q000378:metabolism*; D014877:Water Pollution, Chemical
D000473:Alkanes; D000475:Alkenes; D013431:Sulfates