title: Aggregation and water-membrane partition as major determinants of the activity of the antibiotic peptide trichogin GA IV.
abstract: Water-membrane partition and aggregation behavior are fundamental aspects of the biological activity of antibiotic peptides, natural compounds causing the death of pathogenic organisms by perturbing the permeability of their membranes. A synthetic fluorescent analog of the natural lipopeptaibol trichogin GA IV was used to study its interaction with model membranes. Time-resolved fluorescence data show that in water, an equilibrium between monomers and small aggregates is present, the two species having different affinity for membranes. Therefore, association curves are strongly dependent on peptide concentration. A similar heterogeneity is present in the membrane phase, which strongly suggests the occurrence of a monomer-aggregate equilibrium in this case, too. The relative population of each species was determined and a strong correlation between the concentration of membrane-bound aggregates and membrane leakage was found, thereby suggesting that liposome perturbation is due to peptide aggregates only. Light-scattering measurements demonstrate that leakage is not due to liposome micellization. Moreover, experiments with markers of different sizes show that molecules with a diameter of approximately 4 nm are released only to a minor extent. Overall, these results suggest that, within the concentration range explored, pore formation by peptide aggregates is the most likely mechanism of action for trichogin in membranes.
authors.affiliation: Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata, 00133 Rome, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Stella
authors.forename: Claudia
authors.initials: C
authors.lastname: Mazzuca
authors.forename: Mariano
authors.initials: M
authors.lastname: Venanzi
authors.forename: Antonio
authors.initials: A
authors.lastname: Palleschi
authors.forename: Mara
authors.initials: M
authors.lastname: Didonè
authors.forename: Fernando
authors.initials: F
authors.lastname: Formaggio
authors.forename: Claudio
authors.initials: C
authors.lastname: Toniolo
authors.forename: Basilio
authors.initials: B
authors.lastname: Pispisa
mesh_terms: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry*; D019281:Dimerization; D006020:Glycopeptides; D055666:Lipopeptides; D008081:Liposomes / Q000737:chemistry*; D046911:Macromolecular Substances; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D008567:Membranes, Artificial; D008968:Molecular Conformation; D010455:Peptides / Q000737:chemistry*; D010539:Permeability; D016062:Porosity; D011485:Protein Binding; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D006020:Glycopeptides; D055666:Lipopeptides; D008081:Liposomes; D046911:Macromolecular Substances; D008563:Membrane Lipids; D008565:Membrane Proteins; D008567:Membranes, Artificial; D010455:Peptides; C116419:trichogin GA IV; D014867:Water