title: Establishment of a HPV and p53-mutation-negative human cell line (CA) derived from a squamous carcinoma of the uterine cervix.
abstract: OBJECTIVE
  Well-characterized human cancer cell lines are important research resources for studying cancer cell biology, as well as for developing new strategies against cancer cell growth and progression. We present a new cell line, CA, established from an invasive non-keratinizing squamous cell carcinoma of the uterine cervix in 36-year-old patient.
  
  
  METHODS
  We measured the doubling time of CA cells. To investigate the tumorigenicity of CA, cells were inoculated subcutaneously into the back of nude mice. Several tumor markers were analyzed using culture media by EIA. PCR-based analyses were performed to examine the human papillomavirus (HPV) status and telomerase activity. CA was also screened for p53 mutation using the sequencing technique.
  
  
  RESULTS
  The cells show rapid growth in culture with a doubling time of 14.3 h and high migration activity. Monolayer-cultured cells were polygonal, showing a pavement-like arrangement and a tendency to pile up without contact inhibition. Subcutaneous transplantation of the CA cells into nude mice formed solid tumors that were histologically diagnosed as squamous cell carcinoma, whereas no metastasis was observed. Cultured CA cells produced SCC, CEA, TPA, CA125 and SLX. Genetic and molecular analyses revealed high telomerase activity and the absence of HPV DNA. No p53 mutation was observed in this cell line.
  
  
  CONCLUSIONS
  These properties suggest that CA is an aggressive cervical carcinoma cell line and may serve as a useful experimental model for studying HPV role in cervical carcinogenesis.
authors.affiliation: Department of Obstetrics and Gynecology, Tokyo Medical University, Tokyo 160-0023, Japan. isaka@tokyo-med.ac.jp
authors.forename: Keiichi
authors.initials: K
authors.lastname: Isaka
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Nishi
authors.forename: Yumi
authors.initials: Y
authors.lastname: Osakabe
authors.forename: Machiko
authors.initials: M
authors.lastname: Miyata
authors.forename: Mitsuyasu
authors.initials: M
authors.lastname: Hokamura
authors.forename: Toshihide
authors.initials: T
authors.lastname: Nakada
authors.forename: Osamu
authors.initials: O
authors.lastname: Itokazu
authors.forename: Masaomi
authors.initials: M
authors.lastname: Takayama
mesh_terms: D000328:Adult; D000782:Aneuploidy; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D045744:Cell Line, Tumor; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D009154:Mutation; D009368:Neoplasm Transplantation; D027383:Papillomaviridae; D016133:Polymerase Chain Reaction; D014183:Transplantation, Heterologous; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000821:virology
chemical_list: D014408:Biomarkers, Tumor; D019941:Cyclin-Dependent Kinase Inhibitor p16