title: Probiotics inhibit TNF-alpha-induced interleukin-8 secretion of HT29 cells.
abstract: OBJECTIVE
  To study the effect of probiotics on interleukin-8 secretion in intestinal epithelia when stimulated by proinflammatory cytokines.
  
  
  METHODS
  Colonic adenocarcinoma HT29 cells were cultured and divided into four groups: control, TNF-alpha (group T in short), bifidobacterium (group B), lactobacillus (group L). B. Longum and L. bulgaricus were suspended in culture medium with a concentration of 1 x 10(8) cfu/ml and added into 24 wells respectively. One hour later TNF-alpha (10 ng/ml) was added into each well of groups T, B, L. The supernatants were collected and measured for IL-8 after 3 hours, nuclear factor-kappaB (NF-kappaB) p65 was also examined by Western blotting.
  
  
  RESULTS
  There was less interleukin-8 secretion in HT29 cells when preincubated with B. Longum or L. bulgaricus compared with group T. Less p65 appeared in nuclei in groups B and L compared with group T, as detected by Western blot.
  
  
  CONCLUSIONS
  Probiotics can suppress interleukin-8 secretion in intestinal epithelia when stimulated by proinflammatory cytokines, which is most likely mediated by NF-kappaB.
authors.affiliation: Department of Gastroenterology, Huaxi Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China. baiap@163.com
authors.forename: Ai-Ping
authors.initials: AP
authors.lastname: Bai
authors.forename: Qin
authors.initials: Q
authors.lastname: Ouyang
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Chun-Hui
authors.initials: CH
authors.lastname: Wang
authors.forename: Sheng-Fu
authors.initials: SF
authors.lastname: Li
mesh_terms: D019073:HT29 Cells / Q000378:metabolism*; D006801:Humans; D016209:Interleukin-8 / Q000037:antagonists & inhibitors*; D007413:Intestinal Mucosa / Q000378:metabolism; D019936:Probiotics / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha