title: [Effects of fluoride on proliferation and differentiation of rat osteoblasts in vitro and the antagonistic action of vitamin C].
abstract: OBJECTIVE
  To study the effects of fluoride on the proliferation and differentiation of osteoblasts in sucking rats and the antagonism of vitamin Cin vitro.
  
  
  METHODS
  The enzyme digesting method was used to isolate the rat osteoblasts; the proliferative response was determined by the percents of reduced alamarBlue; the activity of alkaline phosphatase (ALP) was measured by ELISA method.
  
  
  RESULTS
  The proliferation of sucking rat osteoblasts was increased at 0.10 - 1.00 mmol/L of NaF, whereas inhibited at >or= 2.00 mmol/L. ALP activity was increased at 0.01 - 0.05 mmol/L of NaF, and decreased at >or= 0.10 mmol/L. The inhibition on proliferation and differentiation at 2 mmol/L NaF was antagonized by vitamin C.
  
  
  CONCLUSIONS
  Fluoride had a two-phase effect on osteoblasts, vitamin C could antagonize the inhibitory effect of higher concentration of fluoride on proliferation and differentiation of osteoblasts.
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, China Medical University, Shenyang 110001, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Gui-fan
authors.initials: GF
authors.lastname: Sun
authors.forename: Ya-ping
authors.initials: YP
authors.lastname: Jin
authors.forename: Li-hong
authors.initials: LH
authors.lastname: Jia
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D000831:Animals, Newborn; D001205:Ascorbic Acid / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D005459:Fluorides / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000469:Alkaline Phosphatase; D001205:Ascorbic Acid; D005459:Fluorides