Effects of L-fucose and fucose-rich oligo- and polysaccharides (FROP-s) on collagen biosynthesis by human skin fibroblasts. Modulation of the effect of retinol, ascorbate and alpha-tocopherol.
The action of L-fucose and a fucose-rich oligosaccharide (FROP-3) on skin explant cultures and fibroblast cell cultures, alone or together with three vitamins (A, C, E, often used in topical preparations) was studied, using as criteria collagen accumulation and collagen and non-collagen protein biosynthesis. In order to confirm the results observed, several variants of the chemical procedure were used. Collagen accumulation in cell cultures and (3)H-proline incorporation in proteins and collagen gave comparable results. L-Fucose produced a slight but significant inhibition of collagen accumulation and of incorporation of radioactive proline in cell associated collagen and also modified the ratio of excreted (soluble) to cell- or tissue-bound collagen and non-collagen proteins. The oligosaccharide (FROP-3) did not produce a comparable inhibition of collagen biosynthesis. Both L-fucose and FROP-3 modulated the action of the two above-mentioned vitamins in most experimental conditions used. The combined action of the three vitamins (all-trans retinol, ascorbate, alpha-tocopherol) with L-fucose and even more so with FROP-3 can be considered as favourable for the modulation of the biosynthetic activity of fibroblasts.
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Laboratoire Universitaire de Recherche en Ophtalmologie, Faculte de Medecine Broussais-Hôtel Dieu, Universite Pierre et Marie Curie (Paris 6), 1, place du Parvis-Notre-Dame, 75181 cedex 4, Paris, France. broberts@wanadoo.fr
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D001205:Ascorbic Acid / Q000494:pharmacology*; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D004338:Drug Combinations; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005643:Fucose / Q000031:analogs & derivatives / Q000494:pharmacology*; D006801:Humans; D008875:Middle Aged; D009844:Oligosaccharides / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology; D014801:Vitamin A / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000494:pharmacology*
D004338:Drug Combinations; D009844:Oligosaccharides; D011134:Polysaccharides; D014801:Vitamin A; D005643:Fucose; D003094:Collagen; D024502:alpha-Tocopherol; D001205:Ascorbic Acid