title: Improved xylanase production by Trichoderma reesei grown on L-arabinose and lactose or D-glucose mixtures.
abstract: Trichoderma reesei Rut C-30 was grown on eight different natural or rare aldopentoses as the main carbon source and on mixtures of an aldopentose with D-glucose or lactose. The fungal cells consumed all aldopentoses tested, except L-xylose and L-ribose. The highest total xylanase and cellulase activities were achieved when cells were grown on L-arabinose as the main carbon source. The total xylanase activity produced by cells grown on L-arabinose was even higher than that produced by cells grown on an equal amount of lactose. In co-metabolism of D-glucose (15 g l(-1)) and L-arabinose (5 g l(-1)), the total volumetric and specific xylanase productivities were improved (derepressed) approximately 23- and 18-fold, respectively, compared to a cultivation on only D-glucose (20 g l(-1)). In a similar experiment, in which cells were grown on a mixture of lactose and L-arabinose, the xylanase productivity was approximately doubled, compared to a cultivation on only lactose. The cellulase productivities, however, were not improved by the addition of L-arabinose. Compared with a typical industrial fungal enzyme production process with lactose as the main carbon source, better volumetric and specific xylanase productivities were achieved both on a lactose/arabinose mixture and on a glucose/arabinose mixture.
authors.affiliation: Laboratory of Bioprocess Engineering, Helsinki University of Technology, P.O. Box 6100, 02015 Espoo, Finland.
authors.forename: H
authors.initials: H
authors.lastname: Xiong
authors.forename: O
authors.initials: O
authors.lastname: Turunen
authors.forename: O
authors.initials: O
authors.lastname: Pastinen
authors.forename: M
authors.initials: M
authors.lastname: Leisola
authors.forename: N
authors.initials: N
authors.lastname: von Weymarn
mesh_terms: D001089:Arabinose / Q000378:metabolism*; D018533:Biomass; D019149:Bioreactors; D002480:Cellulase / Q000096:biosynthesis / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis / Q000378:metabolism*; D005656:Fungal Proteins / Q000032:analysis / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism*; D007785:Lactose / Q000378:metabolism*; D012266:Ribose / Q000378:metabolism; D014242:Trichoderma / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D012266:Ribose; D014994:Xylose; D001089:Arabinose; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases; D005947:Glucose; D007785:Lactose