Integrated high capacity solid phase extraction-MS/MS system for pharmaceutical profiling in drug discovery.
A method is described for use in analysis of samples from pharmaceutical profiling of early drug discovery compounds. The method consists of a high capacity autosampler which injects samples into one of two solid phase extraction columns operated in parallel for alternating trapping, washing and elution into a tandem quadrupole mass spectrometer operated in multiple reaction monitoring (MRM) MS/MS mode. A primary method, which is useful for 80-90% of compounds, and a secondary method, which is useful for a majority of the remaining compounds, are described. No analytical HPLC column is used and the analysis rate is approximately 50 samples/h. Specificity is obtained using MRM analysis. Application of the method for high capacity analysis of metabolic stability samples is described.
Kerns
Edward H
EH
Wyeth Research, CN8000, Princeton, NJ 08543-8000, USA. kernse@wyeth.com
Kleintop
Teresa
T
Little
David
D
Tobien
Thomas
T
Mallis
Larry
L
Di
Li
L
Hu
Mei
M
Hong
Yan
Y
McConnell
Oliver J
OJ
D015195:Drug Design; D004355:Drug Stability; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000295:instrumentation / Q000379:methods*
D004364:Pharmaceutical Preparations