Demethylbellidifolin inhibits adhesion of monocytes to endothelial cells via reduction of tumor necrosis factor alpha and endogenous nitric oxide synthase inhibitor level.
The effect of demethylbellidifolin (DMB), a major compound of Swertia davidi Franch, on the adhesion of monocytes to endothelial cells induced by oxidized low-density lipoprotein (ox-LDL) was studied. Adhesion of monocytes to endothelial cells was induced by treatment with ox-LDL (100 microg/mL) for 48 h. Levels of tumor necrosis factor-alpha (TNF-alpha) and asymmetric dimethylarginine (ADMA, an endogenous inhibitor of NOS) in conditioned medium and the activity of dimethylarginine dimethylaminohydrolase (DDAH) in endothelial cells were measured. DMB (3 or 10 micromol/L) significantly inhibited the adhesion of monocytes to endothelial cells, attenuated an increase in levels of TNF-alpha and ADMA, and a decrease in the activity of DDAH by ox-LDL. The present results suggest that DMB inhibits the increased adhesion of monocytes to endothelial cells induced by ox-LDL, and that the effect of DMB is related to reduction of the ADMA concentration via reduction of TNF-alpha  production in cultured endothelial cells treated with ox-LDL.
Jiang
De-Jian
DJ
Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha, China.
Jiang
Jun-Lin
JL
Tan
Gui-Shan
GS
Huang
Zhi-Zhuang
ZZ
Deng
Han-Wu
HW
Li
Yuan-Jian
YJ
D002448:Cell Adhesion / Q000187:drug effects; D008078:Cholesterol, LDL; D042783:Endothelial Cells / Q000502:physiology*; D006801:Humans; D009000:Monocytes / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000187:drug effects* / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D029967:Swertia; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects* / Q000378:metabolism; D014966:Xanthenes / Q000008:administration & dosage / Q000494:pharmacology*
D008078:Cholesterol, LDL; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D014966:Xanthenes; C467829:demethylbellidifolin; D019001:Nitric Oxide Synthase