Sequential feeding of glucose and valerate in a fed-batch culture of Ralstonia eutropha for production of poly(hydroxybutyrate-co-hydroxyvalerate) with high 3-hydroxyvalerate fraction.
Several important properties of poly(3-hydroxybutyric-co-3-hydroxyvaleric acids) (P(3HB-co-3HV) depend mainly on the HV unit fraction of the copolymer. Sequential and simultaneous feeding of glucose and valerate were employed to produce P(3HB-co-3HV) in a fed-batch culture of Ralstonia eutropha, and the effects of feeding models on the cell growth, 3HV unit fraction, and copolymer productivity have been investigated. The sequential feeding of glucose and then valerate resulted in a cell density of 110.2 g/L, 3HV unit fraction of 62.7 mol %, and copolymer productivity of 0.56 g/(L.h), while the latter simultaneous feeding strategy never achieved the 3HV fraction of P(3HB-co-3HV) higher than 50%. A nuclear magnetic resonance study confirmed that the production of random copolymer P(3HB-co-3HV) with high 3HV unit fraction was possible even with sequential feeding of glucose and valerate.
Shang
Longan
L
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Korea. Lashang@hotmail.com
Yim
Seong Chun
SC
Park
Hyun Gyu
HG
Chang
Ho Nam
HN
D019149:Bioreactors / Q000382:microbiology*; D002455:Cell Division; D020118:Cupriavidus necator / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D010421:Pentanoic Acids / Q000302:isolation & purification / Q000378:metabolism; D011091:Polyesters / Q000302:isolation & purification / Q000378:metabolism*; D014631:Valerates / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
D005947:Glucose; D010421:Pentanoic Acids; D011091:Polyesters; D014631:Valerates; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C013056:beta-hydroxyvaleric acid