title: Ebselen-mediated protection from single and repeated noise exposure in rat.
abstract: OBJECTIVE
  Ebselen, a glutathione peroxidase mimic, is a candidate compound for the prevention of noise-induced hearing loss.
  
  
  METHODS
  Single-blinded, placebo-controlled study.
  
  
  METHODS
  Methods included single and repeated noise exposures on F-344 female rats given oral or injected ebselen or vehicle before and after noise, evoked auditory brainstem responses using click and pure-tone stimuli, light and fluorescence microscopy of cochleae stained with 4',6-Diamidino-2-phenylindole (DAPI) and fluorescein isothiocyanate-phalloidin, and statistical power determined by ANOVA.
  
  
  RESULTS
  Auditory brainstem response indicated that ebselen provided significant protection from both temporary and permanent threshold shifts following single and repeated noise exposure. On average, three times more outer hair cells were lost in control versus ebselen-treated animals.
  
  
  CONCLUSIONS
  Ebselen reduces noise-induced hearing loss in rats.
authors.affiliation: Sound Pharmaceuticals, Inc, Seattle, WA 98103, USA. elynch@soundpharmaceuticals.com
authors.forename: Eric D
authors.initials: ED
authors.lastname: Lynch
authors.forename: Rende
authors.initials: R
authors.lastname: Gu
authors.forename: Carol
authors.initials: C
authors.lastname: Pierce
authors.forename: Jonathan
authors.initials: J
authors.lastname: Kil
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001393:Azoles / Q000008:administration & dosage / Q000627:therapeutic use*; D003051:Cochlea / Q000166:cytology; D016057:Evoked Potentials, Auditory, Brain Stem; D005260:Female; D006198:Hair Cells, Auditory / Q000166:cytology; D006317:Hearing Loss, Noise-Induced / Q000517:prevention & control*; D054833:Isoindoles; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D016566:Organoselenium Compounds / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D001393:Azoles; D054833:Isoindoles; D018696:Neuroprotective Agents; D016566:Organoselenium Compounds; C042986:ebselen