title: Cancer chemopreventive activity of rotenoids from Derris trifoliata.
abstract: A study of the chemical constituents of the stems of Derris trifoliata Lour. (Leguminosae) led to the isolation and identification of one new rotenoid, 6aalpha,12aalpha-12a-hydroxyelliptone ( 3), together with five other known rotenoids. In a search for novel cancer chemopreventive agents (anti-tumor promoters), we carried out a primary screening of five of the rotenoids isolated from the plant for their inhibitory effects on Epstein-Barr virus early antigen (EBV-EA) activation induced by 12- O-tetradecanoylphorbol 13-acetate (TPA) in Raji cells. The inhibitory activity of 3 was found to be equivalent to that of beta-carotene without any cytotoxicity. Deguelin ( 4) and alpha-toxicarol ( 5) exhibited a marked inhibitory effect on mouse skin tumor promotion in an in vivo two-stage carcinogenesis test. This investigation indicated that rotenoids might be valuable anti-tumor promoters.
authors.affiliation: Faculty of Pharmacy, Meijo University, Nagoya, Japan.
authors.forename: Chihiro
authors.initials: C
authors.lastname: Ito
authors.forename: Masataka
authors.initials: M
authors.lastname: Itoigawa
authors.forename: Naoki
authors.initials: N
authors.lastname: Kojima
authors.forename: Hugh T-W
authors.initials: HT
authors.lastname: Tan
authors.forename: Junko
authors.initials: J
authors.lastname: Takayasu
authors.forename: Harukuni
authors.initials: H
authors.lastname: Tokuda
authors.forename: Hoyoku
authors.initials: H
authors.lastname: Nishino
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Furukawa
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D000956:Antigens, Viral / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D029866:Derris; D004354:Drug Screening Assays, Antitumor; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D018547:Plant Stems; D012402:Rotenone / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D012878:Skin Neoplasms / Q000517:prevention & control
chemical_list: D016588:Anticarcinogenic Agents; D000956:Antigens, Viral; C033305:Epstein-Barr virus early antigen; D010936:Plant Extracts; D012402:Rotenone