A fluorescent analogue of UDP-N-acetylglucosamine: application for FRET assay of peptidoglycan translocase II (MurG).
A direct continuous fluorescence assay for translocase II MurG based on fluorescence resonance energy transfer (FRET) has been developed using a 6-substituted fluorescent analogue of UDP-N-acetylglucosamine.
Li
Jian-Jun
JJ
Department of Chemistry, University of Warwick, Coventry, UK.
Bugg
Timothy D H
TD
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D002384:Catalysis; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D007700:Kinetics; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D015394:Molecular Structure; D017351:N-Acetylglucosaminyltransferases / Q000032:analysis*; D010457:Peptidoglycan / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000737:chemistry*
D001425:Bacterial Outer Membrane Proteins; D005456:Fluorescent Dyes; D008055:Lipids; D010457:Peptidoglycan; D014537:Uridine Diphosphate N-Acetylglucosamine; D017351:N-Acetylglucosaminyltransferases; C064057:UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase