IgE-binding components of cultured human keratinocytes in atopic eczema/dermatitis syndrome and their crossreactivity with Malassezia furfur.
BACKGROUND
Atopic eczema/dermatitis syndrome (AEDS) patients display immunoglobulin E (IgE) reactivity to several antigens, e.g. saprophytic yeasts as Malassezia furfur. AEDS patients also show IgE autoreactivity towards cells of their own tissue including epidermis.


OBJECTIVE
The aim of this study was to investigate the IgE autoreactivity of AEDS patients to cultured keratinocytes and to reveal potential crossreacting epitopes in cultured keratinocytes and M. furfur.


METHODS
Serum samples of 27 AEDS patients were analyzed, of these 13 were M. furfur radioallergosorbent test (RAST) positive and 14 negative. Four urticaria, three psoriasis, and seven nonatopic patients were included as controls. The studies were performed by using IgE immunoblotting and immunoblotting inhibition methods.


RESULTS
Ten IgE-binding protein bands were detected in cultured human keratinocytes by IgE immunoblotting using sera from adult AEDS patients. Anti-keratinocyte IgE antibodies were more associated with elevated S-IgE level than M. furfur RAST. Clear crossreactivity with M. furfur could not be shown.


CONCLUSIONS
The possible pathomechanism of anti-keratinocyte IgE antibodies is not due to IgE epitope mimicry of saprophytic yeast and local tissue in AEDS skin.
Kortekangas-Savolainen
O
O
Departments of Dermatology Medical Biochemistry Clinical Allergology, University of Turku, Turku, Finland.
Peltonen
S
S
Pummi
K
K
Kalimo
K
K
Savolainen
J
J
D000328:Adult; D000918:Antibody Specificity; D000946:Antigens, Fungal / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis; D002478:Cells, Cultured; D003429:Cross Reactions; D003876:Dermatitis, Atopic / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005260:Female; D037502:Galectin 3 / Q000032:analysis; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism*; D015603:Keratinocytes / Q000276:immunology*; D008290:Malassezia / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000276:immunology; D011852:Radioallergosorbent Test; D014581:Urticaria / Q000276:immunology
D000946:Antigens, Fungal; D001323:Autoantibodies; D000939:Epitopes; D037502:Galectin 3; D007073:Immunoglobulin E