title: Proliferation assay on a silicon chip applicable for tumors extirpated from mammalians.
abstract: We describe a novel anticancer drug sensitivity assay on a silicon chip applicable for tumors extirpated from in vivo mammalians. Human promyelocytic leukemia (HL-60) cells were subcutaneously (s.c.) inoculated in SCID mice, then removed 31 days after the inoculation. The cells were embedded in a small volume (18 nL) of a collagen-gel matrix on a pyramid-shaped silicon microstructure for further cultivation. The respiration activity of the cells on the chip was measured by scanning electrochemical microscopy (SECM). The proliferation behavior was continuously monitored for 6 days. It seemed that the proliferation rate of the cells removed from the mice was lower than that cultured in a flask and conformed to that in mice. The effects of cisplatin (CDDP) and etoposide (VP-16) on the HL-60 cultured in vivo were in good agreement with those obtained by a conventional colorimetric assay. Our results suggest that the SECM-based assay is appropriate for biopsy specimens in a relatively short-time evaluation.
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai, Japan.
authors.forename: Yu-suke
authors.initials: YS
authors.lastname: Torisawa
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shiku
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Kasai
authors.forename: Matsuhiko
authors.initials: M
authors.lastname: Nishizawa
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Matsue
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology; D003094:Collagen; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D004563:Electrochemistry / Q000295:instrumentation*; D005047:Etoposide / Q000494:pharmacology; D005260:Female; D018922:HL-60 Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D008838:Microcomputers; D008853:Microscopy; D009374:Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D012825:Silicon
chemical_list: D000970:Antineoplastic Agents; D005047:Etoposide; D003094:Collagen; D002945:Cisplatin; D012825:Silicon