Antibody detection of SARS-CoV spike and nucleocapsid protein.
Early detection and identification of SARS-CoV-infected patients and actions to prevent transmission are absolutely critical to prevent another SARS outbreak. Antibodies that specifically recognize the SARS-CoV spike and nucleocapsid proteins may provide a rapid screening method to allow accurate identification and isolation of patients with the virus early in their infection. For this reason, we raised peptide-induced polyclonal antibodies against SARS-CoV spike protein and polyclonal antibodies against SARS-CoV nucleocapsid protein using 6x His nucleocapsid recombinant protein. Western blot analysis and immunofluorescent staining showed that these antibodies specifically recognized SARS-CoV.
Chang
Mau-Sun
MS
Department of Medical Research, Mackay Memorial Hospital, ROC, Taipei, Taiwan.
Lu
Yen-Ta
YT
Ho
Shin-Tsung
ST
Wu
Chao-Chih
CC
Wei
Tsai-Yin
TY
Chen
Chia-Ju
CJ
Hsu
Yun-Ting
YT
Chu
Po-Chen
PC
Chen
Ching-Hsin
CH
Chu
Jien-Ming
JM
Jan
Ya-Lin
YL
Hung
Chia-Chien
CC
Fan
Chi-Chen
CC
Yang
Yuh-Cheng
YC
D000595:Amino Acid Sequence; D015153:Blotting, Western; D005455:Fluorescent Antibody Technique; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000737:chemistry / Q000276:immunology*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology / Q000302:isolation & purification*; D064370:Spike Glycoprotein, Coronavirus; D013347:Subcellular Fractions; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology*
D008562:Membrane Glycoproteins; D019590:Nucleocapsid Proteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins