A one-step immunochromatographic assay for detecting ginsenosides Rb1 and Rg1.
An immunochromatographic strip test has been developed for detecting ginsenosides Rb1 (G-Rb1) and Rg1 (G-Rg1). This qualitative assay system is useful as a rapid screening method for detecting G-Rb1 and G-Rg1 in plants and plant preparations. Our assay is a competitive immunoassay that uses anti-G-Rb1 and anti-G-Rg1 monoclonal antibodies (MAbs) and a detection reagent that contains colloidal gold particles coated with anti-G-Rb1 and anti-G-Rg1 MAbs. Detection limits are 2 microg mL(-1) for both G-Rb1 and G-Rg1.
Putalun
Waraporn
W
Faculty of Pharmaceutical Sciences, Khon Kaen University, 40002, Khon Kaen, Thailand.
Fukuda
Noriko
N
Tanaka
Hiroyuki
H
Shoyama
Yukihiro
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D000918:Antibody Specificity; D002138:Calibration; D002845:Chromatography / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D036145:Ginsenosides / Q000032:analysis* / Q000276:immunology; D006801:Humans; D008968:Molecular Conformation; D005894:Panax / Q000737:chemistry; D012709:Serum Albumin / Q000276:immunology; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D036145:Ginsenosides; D012709:Serum Albumin; C442759:ginsenoside Rb1; C035054:ginsenoside Rg1