[Comparative proteomics research of apoptosis initiation induced by homoharringtonine in HL-60 cells].
OBJECTIVE
To study the related proteins of apoptosis initiation induced by homoharringtonine (HHT) in HL-60 cells.


METHODS
After establishment of an apoptosis initiation model induced by HHT in HL-60 cells, proteins of untreated and HHT treated HL-60 cells were extracted, and the two-dimensional polyacrylamide gel electrophoresis (2-DE) maps of the extracted proteins were established by using the immobilized pH gradient (IPG) two-dimensional electrophoresis respectively. The alteration protein spots were identified with assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and database searching.


RESULTS
Proteomics analysis showed that proteins including MHC class I antigen, calbindin D-28K, chloride channel protein 6, oncoprotein 18, zinc finger protein Helios and apoptosis inhibitor like protein 2 were involved in apoptosis initiation induced by HHT.


CONCLUSIONS
The present study might conduce to the researches of HL-60 cells carcinogenesis and pave the way to exploit drug precursor related to HHT and initiation of apoptosis in HL-60 cells.
Chen
Chun-yan
CY
Department of Hematological Malignancy, the Second Hospital of Shandong University, Jinan 250033, China.
Jia
Ji-hui
JH
Pan
Xiang-lin
XL
Meng
Yue-sheng
YS
Tu
Zhen-hua
ZH
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D064026:Calbindins; D018118:Chloride Channels / Q000032:analysis; D004268:DNA-Binding Proteins / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D018922:HL-60 Cells; D006248:Harringtonines / Q000494:pharmacology*; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D000077863:Homoharringtonine; D006801:Humans; D051740:Ikaros Transcription Factor; D051034:Inhibitor of Apoptosis Proteins; D008868:Microtubule Proteins; D010750:Phosphoproteins / Q000032:analysis; D011506:Proteins / Q000032:analysis; D020543:Proteome / Q000032:analysis*; D064030:S100 Calcium Binding Protein G / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D050777:Stathmin; D014157:Transcription Factors / Q000032:analysis
D000972:Antineoplastic Agents, Phytogenic; C430870:BIRC8 protein, human; C480840:CLIC6 protein, human; D064026:Calbindins; D018118:Chloride Channels; D004268:DNA-Binding Proteins; D006248:Harringtonines; D015395:Histocompatibility Antigens Class I; C111431:IKZF2 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008868:Microtubule Proteins; D010750:Phosphoproteins; D011506:Proteins; D020543:Proteome; D064030:S100 Calcium Binding Protein G; C493995:STMN1 protein, human; D050777:Stathmin; D014157:Transcription Factors; D051740:Ikaros Transcription Factor; D000077863:Homoharringtonine