[Expression of osteoclast stimulating and differentiating factors in a murine model of localized inflammatory bone resorption].
BACKGROUND
The pathology associated to cholesteatoma is predominantly a consequence of osteoclast-mediated bone resorption within the middle ear. To assess its pathogenesis a murine model for dermal-implant induced osteolysis was evaluated for the expression of osteoclast stimulating and differentiating factors.


METHODS
Mouse calvaria were analysed for the expression of osteoprotegerin ligand (OPGL), osteoprotegerin (OPG) and macrophage-colony stimulating factor (M-CSF) using immunohistochemistry. The detection of osteoclast cell lineage was acquired by immunohistochemistry using markers CD 4, CD 11a, CD 11b, CD 14, CD 51, CD 68 and TRAP.


RESULTS
An increased expression of the investigated cytokines M-CSF, OPG and OPGL was demonstrated by immunohistochemistry. The presence of osteoclast precursor cells and mature resorbing osteoclasts was confirmed in time-dependent manner triggered by dermal implantation.


CONCLUSIONS
This study reveals the basic events in osteoclast biology in localized inflammatory bone resorption and provides new insights into the comprehension of cholesteatoma-induced bone resorption.
Sudhoff
H
H
Hals-Nasen-Ohrenklinik der Ruhr-Universität Bochum, St. Elisabeth Hospital, Bochum, Germany. Holger.Sudhoff@ruhr-uni-bochum.de
Liebehenz
Y
Y
Aschenbrenner
J
J
Euteneuer
S
S
Ebmeyer
J
J
Bernal-Sprekelsen
M
M
Stark
T
T
Dazert
S
S
D000818:Animals; D001862:Bone Resorption; D018424:Cholesteatoma, Middle Ear / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism; D007150:Immunohistochemistry; D008024:Ligands; D016173:Macrophage Colony-Stimulating Factor / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008855:Microscopy, Electron, Scanning; D010010:Osteoclasts; D053244:Osteoprotegerin; D018160:Receptors, Cytoplasmic and Nuclear / Q000276:immunology / Q000378:metabolism; D018124:Receptors, Tumor Necrosis Factor; D016038:Skin Transplantation; D014182:Transplantation, Autologous
D006023:Glycoproteins; D008024:Ligands; D053244:Osteoprotegerin; D018160:Receptors, Cytoplasmic and Nuclear; D018124:Receptors, Tumor Necrosis Factor; C506031:Tnfrsf11b protein, mouse; D016173:Macrophage Colony-Stimulating Factor