title: Two Arabidopsis thaliana genes encode functional pectin methylesterase inhibitors.
abstract: We have identified, expressed and characterized two genes from Arabidopsis thaliana (AtPMEI-1 and AtPMEI-2) encoding functional inhibitors of pectin methylesterases. AtPMEI-1 and AtPMEI-2 are cell wall proteins sharing many features with the only pectin methylesterase inhibitor (PMEI) characterized so far from kiwi fruit. Both Arabidopsis proteins interact with and inhibit plant-derived pectin methylesterases (PMEs) but not microbial enzymes. The occurrence of functional PMEIs in Arabidopsis indicates that a mechanism of controlling pectin esterification by inhibition of endogenous PMEs is present in different plant species.
authors.affiliation: Dipartimento di Biologia Vegetale, Università di Roma 'La Sapienza', Piazzale Aldo Moro 5, 00185 Roma, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Raiola
authors.forename: L
authors.initials: L
authors.lastname: Camardella
authors.forename: A
authors.initials: A
authors.lastname: Giovane
authors.forename: B
authors.initials: B
authors.lastname: Mattei
authors.forename: G
authors.initials: G
authors.lastname: De Lorenzo
authors.forename: F
authors.initials: F
authors.lastname: Cervone
authors.forename: D
authors.initials: D
authors.lastname: Bellincampi
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D001483:Base Sequence; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors*; D002473:Cell Wall / Q000378:metabolism; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D017343:Genes, Plant; D007700:Kinetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; D017931:DNA Primers; D018744:DNA, Plant; D004791:Enzyme Inhibitors; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase