Probiotics inhibit TNF-alpha-induced interleukin-8 secretion of HT29 cells.
OBJECTIVE
To study the effect of probiotics on interleukin-8 secretion in intestinal epithelia when stimulated by proinflammatory cytokines.


METHODS
Colonic adenocarcinoma HT29 cells were cultured and divided into four groups: control, TNF-alpha (group T in short), bifidobacterium (group B), lactobacillus (group L). B. Longum and L. bulgaricus were suspended in culture medium with a concentration of 1 x 10(8) cfu/ml and added into 24 wells respectively. One hour later TNF-alpha (10 ng/ml) was added into each well of groups T, B, L. The supernatants were collected and measured for IL-8 after 3 hours, nuclear factor-kappaB (NF-kappaB) p65 was also examined by Western blotting.


RESULTS
There was less interleukin-8 secretion in HT29 cells when preincubated with B. Longum or L. bulgaricus compared with group T. Less p65 appeared in nuclei in groups B and L compared with group T, as detected by Western blot.


CONCLUSIONS
Probiotics can suppress interleukin-8 secretion in intestinal epithelia when stimulated by proinflammatory cytokines, which is most likely mediated by NF-kappaB.
Bai
Ai-Ping
AP
Department of Gastroenterology, Huaxi Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China. baiap@163.com
Ouyang
Qin
Q
Zhang
Wen
W
Wang
Chun-Hui
CH
Li
Sheng-Fu
SF
D019073:HT29 Cells / Q000378:metabolism*; D006801:Humans; D016209:Interleukin-8 / Q000037:antagonists & inhibitors*; D007413:Intestinal Mucosa / Q000378:metabolism; D019936:Probiotics / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha