title: Rapid identification of Staphylococcus aureus from BacT/ALERT blood culture bottles by direct Gram stain characteristics.
abstract: The rapid identification of Staphylococcus aureus from positive blood cultures provides important clinical and therapeutic information. Using criteria based on direct Gram stain characteristics, an experienced microscopist was able to distinguish S aureus from other staphylococci isolated from BacT/ALERT blood culture bottles with an overall sensitivity of 89% and specificity of 98%. Furthermore, this method was readily taught to a clinical microbiologist who had not previously used the method first hand. Laboratories using the BacT/ALERT blood culture system should become familiar with these criteria so that S aureus bacteraemia can be identified rapidly.
authors.affiliation: Microbiology Unit, Canterbury Health Laboratories, Christchurch 8001, New Zealand. david.murdoch@cdhb.govt.nz
authors.forename: D R
authors.initials: DR
authors.lastname: Murdoch
authors.forename: R L
authors.initials: RL
authors.lastname: Greenlees
mesh_terms: D016470:Bacteremia / Q000175:diagnosis*; D015373:Bacterial Typing Techniques; D002983:Clinical Competence; D005840:Gentian Violet; D006801:Humans; D010619:Phenazines; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000302:isolation & purification
chemical_list: C035863:Gram's stain; D010619:Phenazines; D005840:Gentian Violet