Apc10.1: an ApcMin/+ intestinal cell line with retention of heterozygosity.
APC10.1 is a new intestinal cell line derived from ApcMin/+ mice that retains both the heterozygous Apc genotype and a nonactivated Wnt signaling pathway and displays an early neoplastic phenotype. Although tumorigenic both in immunodepressed and in immunocompetent syngeneic mice, it requires a high cell dose and a long latency. Its epithelial/intestinal origin is shown, in a gene expression profile, by the expression of epithelial transcripts (such as cytokeratin and laminin isoforms) and of developmental regulatory genes (such as Tcf-4, Hnf3beta, p21, Ihh, Hes1) necessary for, or involved in, the maintenance of intestinal stem cells. The lack of activation of the Wnt cascade in APC10.1 cells is shown both by the expression profile of Wnt target genes and by the standard TCF reporter assay. APC10.1 cell line is a novel in vitro model that can contribute to a better understanding of the clinical evolution of familial adenomatous polyposis and to finding of new prophylactic and therapeutic approaches. Supplementary material for this article can be found on the International Journal of Cancer website at http://www.interscience.wiley.com/jpages/0020-7136/suppmat/index.html.
De Giovanni
Carla
C
Cancer Research Section, Department of Experimental Pathology, University of Bologna, Bologna, Italy. degiovanni@cancer.unibo.it
Landuzzi
Lorena
L
Nicoletti
Giordano
G
Astolfi
Annalisa
A
Croci
Stefania
S
Micaroni
Massimo
M
Nanni
Patrizia
P
Lollini
Pier-Luigi
PL
D011125:Adenomatous Polyposis Coli / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D025601:Adenomatous Polyposis Coli Protein / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D017491:Genes, APC; D006579:Heterozygote; D007414:Intestinal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D020411:Oligonucleotide Array Sequence Analysis; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D012334:RNA, Neoplasm / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured; D051153:Wnt Proteins; D029961:Zebrafish Proteins
D025601:Adenomatous Polyposis Coli Protein; D003598:Cytoskeletal Proteins; D011518:Proto-Oncogene Proteins; D012334:RNA, Neoplasm; D014157:Transcription Factors; D051153:Wnt Proteins; D029961:Zebrafish Proteins