Determination of DNA by Rayleigh light scattering enhancement of molecular "light switches".
Base on the enhancement of Rayleigh light scattering signals of molecular "light switches" by DNA under acidic condition, a sensitive and convenient method for DNA determination was proposed. The experiments indicated that, under optimum conditions, good linear relationships were obtained between the Rayleigh light scattering intensity and the concentration of nucleic acids. The detect limits of calf thymus DNA (ctDNA) were 13.0 ng ml(-1), 4.2 ng ml(-1), 51.5 ng ml(-1) and 3.0 ng ml(-1) with four "light switches", respectively. Plasmid DNA extracted from Bacillus subtilis were determined by the proposed method with satisfactory results, and the recovery rates of calf thymus DNA were in the range of 94.6-110.7%.
Chen
Fang
F
College of Chemistry & Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
Huang
Jianping
J
Ai
Xinping
X
He
Zhike
Z
D000818:Animals; D001412:Bacillus subtilis / Q000235:genetics; D002417:Cattle; D004247:DNA / Q000032:analysis*; D004269:DNA, Bacterial / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D049449:Luminescence; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000379:methods
D004269:DNA, Bacterial; D004247:DNA