Structure-based functional discovery of proteins: structural proteomics.
The discovery of biochemical and cellular functions of unannotated gene products begins with a database search of proteins with structure/sequence homologues based on known genes. Very recently, a number of frontier groups in structural biology proposed a new paradigm to predict biological functions of an unknown protein on the basis of its three-dimensional structure on a genomic scale. Structural proteomics (genomics), a research area for structure-based functional discovery, aims to complete the protein-folding universe of all gene products in a cell. It would lead us to a complete understanding of a living organism from protein structure. Two major complementary experimental techniques, X-ray crystallography and NMR spectroscopy, combined with recently developed high throughput methods have played a central role in structural proteomics research; however, an integration of these methodologies together with comparative modeling and electron microscopy would speed up the goal for completing a full dictionary of protein folding space in the near future.
Jung
Jin-Won
JW
Department of Biochemistry and Protein Network Research Center, College of Science, Yonsei University, Seoul 120-749, Korea.
Lee
Weontae
W
D000818:Animals; D018360:Crystallography, X-Ray; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry* / Q000502:physiology* / Q000648:ultrastructure; D020543:Proteome / Q000235:genetics; D040901:Proteomics / Q000379:methods*; D013329:Structure-Activity Relationship
D011506:Proteins; D020543:Proteome