title: Effect of different sterilization processing methods on the mechanical properties of human cancellous bone allografts.
abstract: Use of new sterilization methods applied to human bone is likely to affect both the mechanical and biological properties of human cancellous grafts. The mechanical properties of the transplanted bone inevitably determine the short- and mid-term results of the orthopedic procedure performed. The aim of this study was to compare, under similar conditions, the mechanical effects of gamma irradiation, lipid extraction, and treatment with 6M urea on trabecular bone samples, through conventional mechanical tests and measurement of the ultrasound wave propagation rate. Deteriorations measured for gamma irradiation and lipid extraction were low: 2.4% and 2.5%, respectively, for ultrasound propagation wave measurements. They were clearly significant for protocol including 6M urea, corresponding to a loss of 30% in values measured in the control sample for the stress to failure, inciting prudence when grafted bone is used for support in orthopedic assembly. High consistency in the results obtained between travel time of the ultrasound wave, easily done, and measurement of stress to failure through conventional tests, favor the use of ultrasound protocol, described as a quality test performed on bone grafts in the tissue bank before distribution and implantation.
authors.affiliation: Bone Tissue Banking, Cochin AP-HP, Service de Chirurgie Orthopédique A, Hôpital Cochin, AP-HP, 28 rue du Faubourg Saint Jacques, 75014, Paris Cedex 14, France. laurent.vastel@cch.ap-hop-paris.fr
authors.forename: L
authors.initials: L
authors.lastname: Vastel
authors.forename: A
authors.initials: A
authors.lastname: Meunier
authors.forename: H
authors.initials: H
authors.lastname: Siney
authors.forename: L
authors.initials: L
authors.lastname: Sedel
authors.forename: J-P
authors.initials: JP
authors.lastname: Courpied
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001696:Biomechanical Phenomena / Q000379:methods; D015519:Bone Density / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D016025:Bone Transplantation; D019245:Compressive Strength; D004548:Elasticity; D005270:Femur Head / Q000000981:diagnostic imaging / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D005720:Gamma Rays; D006801:Humans; D066298:In Vitro Techniques; D008055:Lipids / Q000302:isolation & purification; D008875:Middle Aged; D013242:Sterilization / Q000379:methods*; D014021:Tissue Preservation / Q000379:methods*; D014184:Transplantation, Homologous; D014463:Ultrasonography; D014508:Urea / Q000494:pharmacology*
chemical_list: D008055:Lipids; D014508:Urea