title: Effects of various culture environments on expression of major outer membrane proteins from Porphyromonas gingivalis.
abstract: We examined the effects of various culture environments on major outer membrane proteins from Porphyromonas gingivalis ATCC 33277. Major outer membrane protein patterns on gel electrophoresis showed little difference over the culturable range of osmolarity and pH. With elevated temperature or prolonged culture, the intensities of the gingipain bands decreased; however, bands of RagA, RagB and the putative porins were relatively stable. Similar results were observed with several different culture media. Although the precise functions of RagA, RagB and the putative porins are unknown, these factors may be strongly related to the initiation and progression of adult periodontitis.
authors.affiliation: Department of Microbiology, School of Dentistry, Aichi-Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi 464-8650, Japan. yukimu@dpc.aichi-gakuin.ac.jp
authors.forename: Yukitaka
authors.initials: Y
authors.lastname: Murakami
authors.forename: Masashi
authors.initials: M
authors.lastname: Imai
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Mukai
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Ichihara
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakamura
authors.forename: Fuminobu
authors.initials: F
authors.lastname: Yoshimura
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D001426:Bacterial Proteins; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D020559:Monomeric GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D009994:Osmolar Concentration; D016966:Porphyromonas gingivalis / Q000254:growth & development* / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D003470:Culture Media; C484343:RagA protein, Porphyromonas gingivalis; C484346:RagB protein, bacteria; D020559:Monomeric GTP-Binding Proteins