Stem cell-mediated muscle regeneration is enhanced by local isoform of insulin-like growth factor 1.
We investigated the mechanism whereby expression of a transgene encoding a locally acting isoform of insulin-like growth factor 1 (mIGF-1) enhances repair of skeletal muscle damage. Increased recruitment of proliferating bone marrow cells to injured MLC/mIgf-1 transgenic muscles was accompanied by elevated bone marrow stem cell production in response to distal trauma. Regenerating MLC/mIgf-1 transgenic muscles contained increased cell populations expressing stem cell markers, exhibited accelerated myogenic differentiation, expressed markers of regeneration and readily converted cocultured bone marrow to muscle. These data implicate mIGF-1 as a powerful enhancer of the regeneration response, mediating the recruitment of bone marrow cells to sites of tissue damage and augmenting local repair mechanisms.
Musarò
Antonio
A
Department of Histology and Medical Embryology and Centro Eccellenza Biologia e Medicina Molecolare, University of Rome "La Sapienza," 00161 Rome, Italy.
Giacinti
Cristina
C
Borsellino
Giovanna
G
Dobrowolny
Gabriella
G
Pelosi
Laura
L
Cairns
Linda
L
Ottolenghi
Sergio
S
Cossu
Giulio
G
Bernardi
Giorgio
G
Battistini
Luca
L
Molinaro
Mario
M
Rosenthal
Nadia
N
D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology; D039481:CD11b Antigen / Q000032:analysis; D002465:Cell Movement; D007334:Insulin-Like Growth Factor I / Q000502:physiology*; D017493:Leukocyte Common Antigens / Q000032:analysis; D051379:Mice; D008822:Mice, Transgenic; D009132:Muscles / Q000502:physiology*; D020033:Protein Isoforms; D012038:Regeneration / Q000502:physiology*; D013234:Stem Cells / Q000502:physiology*
D039481:CD11b Antigen; D020033:Protein Isoforms; D007334:Insulin-Like Growth Factor I; D017493:Leukocyte Common Antigens