Evidence for acquisition of the lipooligosaccharide biosynthesis locus in Campylobacter jejuni GB11, a strain isolated from a patient with Guillain-Barré syndrome, by horizontal exchange.
Campylobacter jejuni GB11, a strain isolated from a patient with Guillain-Barré syndrome, has been shown to be genetically closely related to the completely sequenced strain C. jejuni NCTC 11168 by various molecular typing and serotyping methods. However, we observed that the lipooligosaccharide (LOS) biosynthesis genes strongly diverged between GB11 and NCTC 11168. We sequenced the LOS biosynthesis locus of GB11 and found that it was nearly identical to the class A LOS locus from the C. jejuni HS:19 Penner serotype strain (ATCC 43446). Analysis of the DNA sequencing data showed that a horizontal exchange event involving at least 14.26 kb had occurred in the LOS biosynthesis locus of GB11 between galE (Cj1131c in NCTC 11168) and gmhA (Cj1149 in NCTC 11168). Mass spectrometry of the GB11 LOS showed that GB11 expressed an LOS outer core that mimicked the carbohydrate portion of the gangliosides GM1a and GD1a, similar to C. jejuni ATCC 43446. The serum from the GB11-infected patient was shown to react with the LOS from both GB11 and ATCC 43446 but not with that from NCTC 11168. These data indicate that the antiganglioside response in the GB11-infected patient was raised against the structures synthesized by the acquired class A LOS locus.
Gilbert
Michel
M
Institute for Biological Sciences, National Research Council Canada, Ottawa, Ontario, Canada. michel.gilbert@nrc.cnrc.gc.ca
Godschalk
Peggy C R
PC
Karwaski
Marie-France
MF
Ang
C Wim
CW
van Belkum
Alex
A
Li
Jianjun
J
Wakarchuk
Warren W
WW
Endtz
Hubert P
HP
D001483:Base Sequence; D016123:Campylobacter jejuni / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D020275:Guillain-Barre Syndrome / Q000276:immunology / Q000382:microbiology*; D006801:Humans; D008070:Lipopolysaccharides / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology; D008969:Molecular Sequence Data
D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides