[Study on the injurious effect of a self designed micro-skin machine on the epithelia].
OBJECTIVE
To observe the injury on micro-skin induced by a self designed micro-skin machine.


METHODS
Micro-skin was produced either with the machine or by hand. Cells at the edge of micro-skin were observed by transmission electron microscope. succinic dehydrogenase activity in supernatant of cultivated cells was analyzed, and the cell proliferation of micro-skin was assessed by (3)H-TdR. Twenty patients were enrolled in the study for the observation of the wound healing time between the two groups of micro-skin after being grafted.


RESULTS
Transmission electron microscope examination revealed that the cellular injury at the edge of the micro-skin in machine-made group was mild compared with that in man-made group. (3)H-TdR rate was elevated but the activity of succinic dehydrogenase in the supernatant of cultured cells decreased in supernatant of cultured cells of machine produced micro-skin. Wound healing time was shortened in machine made group. (P < 0.05).


CONCLUSIONS
The cellular injury at the edge of micro-skin in the machine made group was mild when compared with that in the man-made group with cell proliferation accelerated and wound healing time shortened.
Chen
Jian-she
JS
Department of Burns, The 187th Hospital of PLA, Haikou 571159, Hainan Province, P.R. China.
Chen
Jin-song
JS
Liu
Xian-zhi
XZ
Zhang
Zong-ren
ZR
Shen
Guang-yu
GY
Duan
Hong-jie
HJ
Su
Yong-yue
YY
Liu
Yue-ming
YM
Lv
Gen-fa
GF
D002056:Burns / Q000601:surgery*; D002455:Cell Division; D004848:Epithelium / Q000473:pathology; D006801:Humans; D008854:Microscopy, Electron; D012867:Skin / Q000648:ultrastructure; D016038:Skin Transplantation / Q000379:methods*; D014945:Wound Healing