Global mapping of the yeast genetic interaction network.
A genetic interaction network containing approximately 1000 genes and approximately 4000 interactions was mapped by crossing mutations in 132 different query genes into a set of approximately 4700 viable gene yeast deletion mutants and scoring the double mutant progeny for fitness defects. Network connectivity was predictive of function because interactions often occurred among functionally related genes, and similar patterns of interactions tended to identify components of the same pathway. The genetic network exhibited dense local neighborhoods; therefore, the position of a gene on a partially mapped network is predictive of other genetic interactions. Because digenic interactions are common in yeast, similar networks may underlie the complex genetics associated with inherited phenotypes in other organisms.
Tong
Amy Hin Yan
AH
Banting and Best Department of Medical Research, University of Toronto, Toronto, ON, Canada M5G 1L6.
Lesage
Guillaume
G
Bader
Gary D
GD
Ding
Huiming
H
Xu
Hong
H
Xin
Xiaofeng
X
Young
James
J
Berriz
Gabriel F
GF
Brost
Renee L
RL
Chang
Michael
M
Chen
YiQun
Y
Cheng
Xin
X
Chua
Gordon
G
Friesen
Helena
H
Goldberg
Debra S
DS
Haynes
Jennifer
J
Humphries
Christine
C
He
Grace
G
Hussein
Shamiza
S
Ke
Lizhu
L
Krogan
Nevan
N
Li
Zhijian
Z
Levinson
Joshua N
JN
Lu
Hong
H
Ménard
Patrice
P
Munyana
Christella
C
Parsons
Ainslie B
AB
Ryan
Owen
O
Tonikian
Raffi
R
Roberts
Tania
T
Sdicu
Anne-Marie
AM
Shapiro
Jesse
J
Sheikh
Bilal
B
Suter
Bernhard
B
Wong
Sharyl L
SL
Zhang
Lan V
LV
Zhu
Hongwei
H
Burd
Christopher G
CG
Munro
Sean
S
Sander
Chris
C
Rine
Jasper
J
Greenblatt
Jack
J
Peter
Matthias
M
Bretscher
Anthony
A
Bell
Graham
G
Roth
Frederick P
FP
Brown
Grant W
GW
Andrews
Brenda
B
Bussey
Howard
H
Boone
Charles
C
D000595:Amino Acid Sequence; D019295:Computational Biology; D003550:Cystic Fibrosis / Q000235:genetics; D017353:Gene Deletion; D020043:Genes, Essential; D005800:Genes, Fungal; D030342:Genetic Diseases, Inborn / Q000235:genetics; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D020412:Multifactorial Inheritance; D009154:Mutation; D010641:Phenotype; D011110:Polymorphism, Genetic; D012174:Retinitis Pigmentosa / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D029701:Saccharomyces cerevisiae Proteins