Improved display of synthetic IgG-binding domains on the baculovirus surface.
Improved display of foreign protein moieties in combination with beneficial alteration of the viral surface properties should be of value for targeted and enhanced gene delivery. Here, we describe a vector based on Autographa californica multiple nucleopolyhedrovirus (AcMNPV) displaying synthetic IgG-binding domains (ZZ) of protein A fused to the transmembrane anchor of vesicular stomatitis virus (VSV) G protein. This display vector was equipped with a GFP/EGFP expression cassette enabling fluorescent detection in both insect and mammalian cells. The virus construct displayed the biologically active fusion protein efficiently and showed increased binding capacity to IgG. As the display is carried out using a membrane anchor of foreign origin, gp64 is left intact for virus entry, which may increase gene expression in the transduced mammalian cells. In addition, the viral vector can be targeted to any desired cell type via binding of ZZ domains when an appropriate IgG antibody is available.
Ojala
Kirsi
K
University of Jyvaskyla, Department of Biological and Environmental Science, University of Jyvaskyla, POB 35, FIN-40014, Finland.
Koski
Johanna
J
Ernst
Wolfgang
W
Grabherr
Reingard
R
Jones
Ian
I
Oker-Blom
Christian
C
D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000648:ultrastructure; D001666:Binding Sites, Antibody / Q000235:genetics* / Q000276:immunology*; D002460:Cell Line; D006224:Cricetinae; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics; D017924:Nucleopolyhedroviruses / Q000235:genetics; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000648:ultrastructure; D018411:Spodoptera; D014721:Vesicular stomatitis Indiana virus; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics
C028391:G protein, vesicular stomatitis virus; D007106:Immune Sera; D007074:Immunoglobulin G; D008562:Membrane Glycoproteins; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins