title: Abnormal response to ryanodine in oesophageal striated muscle of spontaneously hypertensive rats.
abstract: The effects of ryanodine on twitch contraction and basal tension of oesophageal striated muscle were compared between preparations from stroke-prone spontaneously hypertensive rats (SHRSP) and normotensive Wistar Kyoto rats (WKY). Ryanodine (3 x 10(-7) M) augmented the twitch contraction in WKY preparations, butt attenuated it in SHRSP preparations. Rates of contraction and relaxation of twitch contraction, normalized to developed tension, were slightly decreased by ryanodine in both preparations. The effect of ryanodine was not different between WKY and SHRSP preparations. Ryanodine elevated the basal tension in WKY preparations but not in SHRSP preparations. Ryanodine elevated the intracellular Ca(2+) level in both preparations, but the response was significantly less in SHRSP preparations. Resting and action potentials were not significantly different between WKY and SHRSP preparations, while the duration of the action potential was significantly longer in SHRSP preparations. Ryanodine did not alter the resting and action potentials of either preparation. These results suggest that the Ca(2+) handling properties, including the ryanodine receptor, of the sarcoplasmic reticulum are genetically altered in oesophageal striated muscle of SHRSP.
authors.affiliation: Division of Physiology and Pathophysiology, School of Pharmaceutical Sciences, Kinki University, 3-4-1 Kowakae, Higashi-Osaka, Osaka 577-8502, Japan. fmiko@phar.kindai.ac.jp
authors.forename: Fumiko
authors.initials: F
authors.lastname: Sekiguchi
authors.forename: Kyoko
authors.initials: K
authors.lastname: Kawata
authors.forename: Masako
authors.initials: M
authors.lastname: Yamazoe
authors.forename: Satoru
authors.initials: S
authors.lastname: Sunano
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D002110:Caffeine; D002118:Calcium / Q000378:metabolism; D004947:Esophagus / Q000187:drug effects* / Q000502:physiology; D006973:Hypertension / Q000503:physiopathology*; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012433:Ryanodine / Q000494:pharmacology*; D013045:Species Specificity
chemical_list: D012433:Ryanodine; D002110:Caffeine; D002118:Calcium