Use of group-specific and RAPD-PCR analyses for rapid differentiation of Lactobacillus strains from probiotic yogurts.
The increasing interest in probiotic lactobacilli implicates the requirement of techniques that allow a rapid and reliable identification of these organisms. In this study, group-specific PCR and RAPD-PCR analyses were used to identify strains of the Lactobacillus casei and Lactobacillus acidophilus groups most commonly used in probiotic yogurts. Group-specific PCR with primers for the L. casei and L. acidophilus groups, as well as L. gasseri/johnsonii, could differentiate between 20 Lactobacillus strains isolated from probiotic yogurts and assign these into the corresponding groups. For identification of these strains to species or strain level, RAPD profiles of the 20 Lactobacillus strains were compared with 11 reference strains of the L. acidophilus and L. casei group. All except one strain could be attributed unambigously to the species L. acidophilus, L. johnsonii, L. crispatus, L. casei, and L. paracasei. DNA reassociation analysis confirmed the classification resulting from the RAPD-PCR.
Schillinger
Ulrich
U
Federal Research Centre for Nutrition, Institute of Hygiene and Toxicology, Haid-und-Neu Strasse 9, D-76131 Karlsruhe, Germany. Ulrich.Schillinger@bfe.uni-karlsruhe.de
Yousif
Nuha M K
NM
Sesar
Lilijana
L
Franz
Charles M A P
CM
D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D005516:Food Microbiology; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D007779:Lactobacillus acidophilus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D007780:Lacticaseibacillus casei / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D019936:Probiotics; D019105:Random Amplified Polymorphic DNA Technique; D015014:Yogurt / Q000382:microbiology*
D004269:DNA, Bacterial