[Susceptibility change to HBV in primary culture of first trimester human fetal hepatocytes].
OBJECTIVE
By culturing primary early (8 to 12-weeks-old) human fetal hepatocytes with different conditions, to study the status of cell susceptibility to HBV.


METHODS
During primary culture of 10-weeks-old human fetal hepatocytes with serum-free medium adding different differentiation-induced ingredients, to inoculate cell with HBV at certain time. Cell shape, function and markers of HBV infection are measured.


RESULTS
6 days after seeding, markers of mature hepatocytes are observed in cells cultured with 2.5mmol/L phenobarbital sodium, and these cells show susceptibility to HBV. Other ingredients cannot render hepatocytes susceptible to HBV.


CONCLUSIONS
Phenobarbital sodium induces differentiation and susceptibility to HBV in primary culture of early human fetal hepatocytes.
Tang
Bo
B
Department of Infectious Diseases, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Wang
Yu-ming
YM
Wang
Fang
F
Liu
Jun
J
Zhang
Rui
R
D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D005333:Fetus / Q000821:virology*; D006515:Hepatitis B virus / Q000254:growth & development*; D022781:Hepatocytes / Q000166:cytology / Q000502:physiology / Q000821:virology*; D006801:Humans; D010634:Phenobarbital / Q000494:pharmacology; D011247:Pregnancy; D011261:Pregnancy Trimester, First
D010634:Phenobarbital