title: Characterization of Borrelia burgdorferi sensu lato from Novosibirsk region (West Siberia, Russia) based on direct PCR.
abstract: Borrelia burgdorferi sensu lato infecting Ixodes persulcatus ticks near Novosibirsk, Russia were detected using PCR with primers specific to 5S and 23S rRNA genes. Two genospecies, B. afzelii and B. garinii, were identified by the PCR-based restriction fragment length polymorphism analysis with Tru9-I restriction endonuclease. Comparison of the corresponding nucleotide sequences revealed considerable diversity of the 5S-23S intergenic spacer structure among B. garinii.
authors.affiliation: Institute of Systematics and Ecology of Animals, Novosibirsk, Russia. dob@eco.nsc.ru
authors.forename: Nnatalya Nikolaevna
authors.initials: NN
authors.lastname: Livanova
authors.forename: O'lga Vladimirovich
authors.initials: OV
authors.lastname: Morozova
authors.forename: Igor' Vladimirovich
authors.initials: IV
authors.lastname: Morozov
authors.forename: Anatoly Bonsovich
authors.initials: AB
authors.lastname: Beklemishev
authors.forename: Felipe C
authors.initials: FC
authors.lastname: Cabello
authors.forename: Andrey Kirovich
authors.initials: AK
authors.lastname: Dobrotvorsky
mesh_terms: D000818:Animals; D001483:Base Sequence; D015748:Borrelia burgdorferi Group / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers; D018884:Ixodes / Q000382:microbiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012341:RNA, Ribosomal, 5S; D012689:Sequence Homology, Nucleic Acid; D012800:Siberia
chemical_list: D017931:DNA Primers; D012338:RNA, Ribosomal, 23S; D012341:RNA, Ribosomal, 5S