title: Real-time 2D NMR identification of analytes undergoing continuous chromatographic separation.
abstract: We have recently proposed and demonstrated an approach that enables the acquisition of multidimensional nuclear magnetic resonance (NMR) spectra within a single scan. A promising application opened up by this new accelerated form of data acquisition concerns the possibility of monitoring in real time the chemical nature of analytes subject to a continuous flow. The present paper illustrates such potential, with the real-time acquisition of a series of 2D 1H NMR spectra arising from a mixture of compounds subject to a continuous liquid chromatography (LC) separation. This real-time 2D NMR identification of chemicals eluted minutes apart under usual LC-NMR conditions differs from the way in which LC-2D NMR has hitherto been carried out, which relies on stopped-flow modes of operations whereby fractions are first collected and then subject to individual, aliquot-by-aliquot analyses. The real-time LC-2D NMR experiment hereby introduced can be implemented in a straightforward manner using modern commercial LC-NMR hardware, thus opening up immediate possibilities in high-throughput characterizations of complex molecules.
authors.affiliation: Department of Chemical Physics, Weizmann Institute of Science, 76100 Rehovot, Israel.
authors.forename: Boaz
authors.initials: B
authors.lastname: Shapira
authors.forename: Amir
authors.initials: A
authors.lastname: Karton
authors.forename: Dina
authors.initials: D
authors.lastname: Aronzon
authors.forename: Lucio
authors.initials: L
authors.lastname: Frydman
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000295:instrumentation / Q000379:methods*