Cell micropatterning using photopolymerization with a liquid crystal device commercial projector.
Photopolymerization has been widely used for surface micropatterning. The technique often requires photomasks and light sources with appropriate energies or filters. For rapid prototyping of surface photo-micropatterning, we have developed a novel device by modifying a commercially available liquid crystal device projector. In place of the image expansion unit of the projector, we attached an image reduction unit, an adjustable stage, and an optical monitoring unit. The device projected computer-generated images onto surfaces and subjected these patterns to photopolymerization. Micropatterned images can be easily prepared with various software run on personal computers. With the developed photopolymerization device, micropatterning of poly(ethylene glycol) (PEG) was achieved with PEG-diacrylate and a visible light photopolymerization initiator, camphorquinone. Selective cell adhesion control was also achieved on the micropatterned surfaces.
Itoga
Kazuyoshi
K
Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-Cho, Shinjuku-ku, Tokyo 162-8666, Japan.
Yamato
Masayuki
M
Kobayashi
Jun
J
Kikuchi
Akihiko
A
Okano
Teruo
T
D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D016287:Computer Peripherals; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology*; D019544:Equipment Failure Analysis; D008422:Materials Testing; D010777:Photochemistry / Q000295:instrumentation* / Q000379:methods; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis; D013499:Surface Properties
D001672:Biocompatible Materials; D011108:Polymers; D011092:Polyethylene Glycols