title: Crystallization and preliminary crystallographic analysis of rat monoamine oxidase A complexed with clorgyline.
abstract: Monoamine oxidase (MAO) is an FAD-containing mitochondrial outer-membrane protein which catalyzes the degradation of several neurotransmitters in the central nervous system. The two subtypes of MAO, MAOA and MAOB, have similar primary sequences but different substrate and inhibitor specificities. The structure of human MAOB has recently been determined, but the structure of MAOA remains unknown. To clarify the mechanisms underlying their unique substrate and inhibitor recognition and thereby facilitate the development of new specific inhibitors to treat MAO-related neurological disorders, rat MAOA was crystallized in a complex with the specific inhibitor clorgyline. Diffraction data were collected to 3.2 A resolution. The crystal belongs to the space group P4(3)2(1)2, with unit-cell parameters a = b = 158.2, c = 258.4 A.
authors.affiliation: Institute for Protein Research, Osaka University, Japan.
authors.forename: Jichun
authors.initials: J
authors.lastname: Ma
authors.forename: Fumie
authors.initials: F
authors.lastname: Kubota
authors.forename: Masato
authors.initials: M
authors.lastname: Yoshimura
authors.forename: Eiki
authors.initials: E
authors.lastname: Yamashita
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nakagawa
authors.forename: Akio
authors.initials: A
authors.lastname: Ito
authors.forename: Tomitake
authors.initials: T
authors.lastname: Tsukihara
mesh_terms: D000818:Animals; D003010:Clorgyline / Q000737:chemistry* / Q000494:pharmacology; D018360:Crystallography, X-Ray; D003902:Detergents / Q000494:pharmacology; D008995:Monoamine Oxidase / Q000737:chemistry*; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D011485:Protein Binding; D011487:Protein Conformation; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D003902:Detergents; D008996:Monoamine Oxidase Inhibitors; D008995:Monoamine Oxidase; D003010:Clorgyline