title: Cellular accumulation of dietary anticarcinogenic isothiocyanates is followed by transporter-mediated export as dithiocarbamates.
abstract: Many dietary isothiocyanates (ITCs) are potent anticarcinogenic agents. ITCs rapidly accumulate to high concentrations in cells as a result of conjugation with intracellular thiols, especially glutathione (GSH). The anticarcinogenic activity of ITCs depends on, at least partly, their accumulation in cells. We report that three major anticarcinogenic ITCs, including allyl-ITC, benzyl-ITC, and phenethyl-ITC, were rapidly exported, upon accumulation in cells, mainly in the forms of GSH- and cysteinylglycine-conjugates, apparently involving MRP-1 and Pgp-1. These findings are consistent with our previous results regarding cellular export of another anticarcinogenic ITC, sulforaphane, and suggest a common cellular response to ITCs.
authors.affiliation: Arizona Cancer Center, College of Medicine, University of Arizona, Tucson, AZ 85724, USA.
authors.forename: Eileen C
authors.initials: EC
authors.lastname: Callaway
authors.forename: Yuesheng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wade
authors.initials: W
authors.lastname: Chew
authors.forename: H-H Sherry
authors.initials: HH
authors.lastname: Chow
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D016588:Anticarcinogenic Agents / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D018922:HL-60 Cells / Q000378:metabolism; D006801:Humans; D017879:Isothiocyanates / Q000378:metabolism*; D021381:Protein Transport; D013859:Thiocarbamates / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D016588:Anticarcinogenic Agents; D017879:Isothiocyanates; D013859:Thiocarbamates; D005982:Glutathione Transferase; D005978:Glutathione