title: Involvement of highly polysialylated neural cell adhesion molecule (PSA-NCAM)-positive granule cells in the amygdaloid-kindling-induced sprouting of a hippocampal mossy fiber trajectory.
abstract: The mossy fiber system in the hippocampus of amygdaloid-kindled rats was examined by using highly polysialylated neural cell adhesion molecule (PSA-NCAM) as a marker for immunohistochemical detection of immature dentate granule cells and mossy fibers in combination with bromodeoxyuridine (BrdU) labeling of newly generated granule cells. Statistically significant increases in BrdU-labeled cells and PSA-NCAM-positive cells occurred in the dentate gyrus following kindling. The increase in PSA-NCAM-immunoreactive neurites was confined to the entire stratum lucidum of CA3. Immunoelectron-microscopic examination also revealed that PSA-NCAM-positive immature synaptic terminals of the sprouting mossy fibers increased in the stratum lucidum of CA3 in the kindled rats. The increase in the numbers of PSA-NCAM-positive granule cells correlated well with the increase in the immunopositive neurites and synaptic terminals on the mossy fiber trajectory. The increase in these PSA-NCAM-immunopositive structures is thought to reflect the enhancement of sprouting and synaptogenesis of mossy fibers by a subset of granule cells newly generated during amygdaloid-kindling and suggests that the reorganization of the mossy fiber system on the normal trajectory at least in part contributes to the acquisition and maintenance of an epileptogenic state.
authors.affiliation: Department of Neurological Surgery, Graduate School of Medicine, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba 260-8670, Japan. saegusa-nsu@umin.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Saegusa
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Mine
authors.forename: Hiroto
authors.initials: H
authors.lastname: Iwasa
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Murai
authors.forename: Tatsunori
authors.initials: T
authors.lastname: Seki
authors.forename: Akira
authors.initials: A
authors.lastname: Yamaura
authors.forename: Shigeki
authors.initials: S
authors.lastname: Yuasa
mesh_terms: D000679:Amygdala / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D006624:Hippocampus / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D007696:Kindling, Neurologic / Q000378:metabolism*; D008297:Male; D019599:Mossy Fibers, Hippocampal / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*; D039842:Neural Cell Adhesion Molecule L1 / Q000032:analysis / Q000096:biosynthesis / Q000502:physiology*; D009434:Neural Pathways / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012794:Sialic Acids / Q000032:analysis / Q000096:biosynthesis / Q000502:physiology*
chemical_list: D039842:Neural Cell Adhesion Molecule L1; D012794:Sialic Acids; C103469:polysialyl neural cell adhesion molecule