title: Morphological characterization of mouse B-1 cells.
abstract: At least three B cell subsets, B-1a, B-1b and B-2 are present circulating peripherally in the mouse. In these animals, B-1 cells constitute a minor fraction of B cells in spleen and are absent in lymph nodes although they represent the main B cell population in peritoneal and pleural cavities. Currently these cells are identified by a surface phenotypic repertoire; they express Mac-1, IgM(high), and B220(low). B-1a cells express CDS. The aim of this work emerged from the fact that the morphology of B-1 cells is not fully characterized. Here we identified B-1 cells using colloidal gold immunocytochemical assays and purified B-1 cells from supernatants of adherent peritoneal cell cultures by a magnetic bead technique. These techniques lead us to demonstrate that, in mice, either B-1a or B-1b cells have a unique morphology distinct from that of B-2 cells.
authors.affiliation: Disciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia - Universidade Federal de São Paulo, Rua Botucatu, 862 Ed. de Ciências Biomédicas 4o andar, 04023-900 São Paulo, Brazil.
authors.forename: Thalita Balsamo
authors.initials: TB
authors.lastname: Abrahão
authors.forename: Edna
authors.initials: E
authors.lastname: Freymüller
authors.forename: Renato Arruda
authors.initials: RA
authors.lastname: Mortara
authors.forename: José Daniel
authors.initials: JD
authors.lastname: Lopes
authors.forename: Mario
authors.initials: M
authors.lastname: Mariano
mesh_terms: D000818:Animals; D016175:B-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology* / Q000648:ultrastructure; D018956:CD5 Antigens / Q000378:metabolism; D002448:Cell Adhesion; D002467:Cell Nucleus / Q000648:ultrastructure; D005260:Female; D007075:Immunoglobulin M / Q000378:metabolism; D007150:Immunohistochemistry; D018189:Immunomagnetic Separation; D017493:Leukocyte Common Antigens / Q000378:metabolism; D016177:Macrophage-1 Antigen / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D010529:Peritoneal Cavity / Q000166:cytology
chemical_list: D018956:CD5 Antigens; D007075:Immunoglobulin M; D016177:Macrophage-1 Antigen; D017493:Leukocyte Common Antigens