Cross-species reactivity of seven monoclonal antibodies with equine lymphocytes by flow cytometry.
The recognition of equine lymphocyte antigens by monoclonal antibodies (mAbs) directed against human CD11a, CD18, CD21, CD23, CD29 and DR, as well as mouse CD23 was studied by flow cytometry. Unlike anti-CD11a, -CD21, -CD23 and DR mAbs, anti-CD18 and CD29 mAbs labelled the same percentage of horse peripheral blood lymphocytes (PBL) as human PBL. Double-staining with anti-horse immunoglobulin antibodies showed that anti-CD21 and -CD23 mAbs are mainly bound to peripheral blood B lymphocytes. The seven mAbs were also tested on the lymph node and thymus cells. The molecular targets of anti-CD11a, CD18 and CD29 mAbs were confirmed by immunoprecipitation of the membrane proteins. Our results suggest that anti-CD18, -CD29 and -DR mAbs recognise similarly expressed molecular homologues on equine cells, but that anti-CD11a, -CD21 and -CD23 mAbs recognise either different molecules or homologues that are expressed at different levels on horse cells.
Mérant
Catherine
C
Laboratoire d'Immunopathologie, Service de Médecine, Département des Animaux de Compagnie, Ecole Vétérinaire de Lyon, BP 83, 1 avenue Bourgelat, 69280 Marcy-l'Etoile, France. c.merant@ploufragan.afssa.fr
Bonnefont
Catherine
C
Desbos
Agnès
A
Greenland
Timothy
T
Cadoré
Jean-Luc
JL
Monier
Jean-Claude
JC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015703:Antigens, CD / Q000276:immunology*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005434:Flow Cytometry / Q000379:methods / Q000662:veterinary; D006736:Horses / Q000097:blood / Q000276:immunology*; D006801:Humans; D016023:Integrins / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D011233:Precipitin Tests / Q000662:veterinary; D013045:Species Specificity; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D016023:Integrins