Application of electrophoretic methods for detection of protein-porphyrin complexes.
Simple methods for detection and isolation of protein-porphyrin complexes were elaborated in our laboratory. They are based on the separation of protein-porphyrin complexes in native polyacrylamide gel and measurement of their fluorescence, with the use of two detection systems: the commercially available Gel Doc(TM) 2000 system, and a system specially designed for the purpose of these investigations, concerning protein-porphyrin interactions. The fluorescent complexes can be electro-transferred from the gel onto PVDF membrane, eluted and analyzed in order to identify the protein interacting with porphyrins.
Kowalska
Agnieszka
A
Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland. agak@biotech.univ.gda.pl
Kwiek
Piotr
P
Miłosz
Ewa
E
Gondek
Grzegorz
G
Romiszewska
Anna
A
Graczyk
Alfreda
A
Podhajska
Anna J
AJ
D004591:Electrophoresis, Polyacrylamide Gel; D011145:Polyvinyls; D011166:Porphyrins / Q000032:analysis* / Q000378:metabolism; D011485:Protein Binding / Q000502:physiology; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D005465:Fluorocarbon Polymers
D011145:Polyvinyls; D011166:Porphyrins; D011506:Proteins; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers