Effect of tempol (4-hydroxy tempo) on gentamicin-induced nephrotoxicity in rats.
We investigated the effects of tempol (4-hydroxy tempo), a membrane-permeable radical scavenger, on gentamicin-induced renal failure in rats. The rats were given gentamicin (100 mg/kg/day, i.p., once a day); and gentamicin (100 mg/kg/day, i.p.) and tempol (3.5, 7 or 14 mg/kg/day, i.p., once a day). At the end of 7 days, the gentamicin group produced the remarkable nephrotoxicity, characterized by a significantly decreased creatinine clearance and increased serum creatinine, blood urea nitrogen (BUN) and daily urine volume when compared with controls. In control the BUN value was 21.2 +/- 0.07 (mg/100 mL); in comparison, it was 96.9 +/- 6.03 in gentamicin group (P < 0.05). Renal histopathologic examination confirmed acute tubular necrosis in this group. In rats treated with gentamicin and tempol a partial improvement in biochemical and histologic parameters was observed. BUN values were 96.9 +/- 6.03 and 36.3 +/- 2.39 in gentamicin, and gentamicin plus tempol (14 mg/kg) treated groups, respectively (P < 0.05). These results suggest that the administration of tempol may have a protective effect on gentamicin-induced nephrotoxicity in rats.
Karataş
Yusuf
Y
Department of Pharmacology, Medical Faculty, Cukurova University, 01330 Adana, Turkey. ykaratas@cu.edu.tr
Seçilmiş
M Ata
MA
Karayaylali
Ibrahim
I
Doran
Figen
F
Büyükafşar
Kansu
K
Singirik
Ergin
E
Sagliker
Yahya
Y
Dikmen
Atilla
A
D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D003497:Cyclic N-Oxides / Q000494:pharmacology*; D005839:Gentamicins / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D007668:Kidney / Q000473:pathology; D007676:Kidney Failure, Chronic / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013113:Spin Labels; D013482:Superoxide Dismutase / Q000378:metabolism; D016707:Tissue Fixation
D000900:Anti-Bacterial Agents; D003497:Cyclic N-Oxides; D005839:Gentamicins; D013113:Spin Labels; D003404:Creatinine; D013482:Superoxide Dismutase; C001803:tempol