title: Impaired T cell proliferation and zeta chain phosphorylation after stimulation with staphylococcal enterotoxin-B in hemodialysis patients.
abstract: BACKGROUND
  Patients on regular hemodialysis treatment are in an immunodeficiency state. Several studies have shown defective T cell proliferation after stimulation with various agents. Staphylococcal enterotoxin B (SEB) is a MHC-dependent superantigen that triggers proliferation of a large proportion of T cells. T cell activation after stimulation with SEB parallels normal T cell signal transduction. An important and early event in this transduction pathway is the phosphorylation of the zeta chain. In this study, T cell proliferation and zeta chain phosphorylation after stimulation with SEB were evaluated.
  
  
  METHODS
  Peripheral blood mononuclear cells (PBMCs) from 24 patients and 14 healthy individuals were isolated and cultured with or without stimulation with SEB (1 ng/ml). Cell proliferation was estimated by immunoenzymatic measurement of bromodeoxyuridine uptake. PBMCs from 8 patients and 6 healthy individuals were isolated and pulsed for 2 min with or without SEB (10 microg/ml). Zeta chain phosphorylation was estimated by immunoprecipitation and immunoblotting with antiphosphotyrosine antibody.
  
  
  RESULTS
  Lymphocyte proliferation index after SEB stimulation was lower in hemodialyzed patients. Stimulation of T cells with SEB also resulted in a lower zeta chain phosphorylation in hemodialyzed patients.
  
  
  CONCLUSIONS
  Lymphocyte proliferation after MHC-dependent stimulation is impaired in hemodialyzed patients. This proliferation defect is due to impaired zeta chain phosphorylation.
authors.affiliation: Department of Nephrology, B' IKA Hospital, Thessaloniki, Greece. elefthe@otenet.gr
authors.forename: Theodoros
authors.initials: T
authors.lastname: Eleftheriadis
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Papazisis
authors.forename: Alexandros
authors.initials: A
authors.lastname: Kortsaris
authors.forename: Georgios
authors.initials: G
authors.lastname: Vayonas
authors.forename: Sultana
authors.initials: S
authors.lastname: Voyatzi
authors.forename: Vasilios
authors.initials: V
authors.lastname: Vargemezis
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004768:Enterotoxins / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000276:immunology* / Q000628:therapy; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008213:Lymphocyte Activation; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008875:Middle Aged; D010766:Phosphorylation; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D006435:Renal Dialysis; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism
chemical_list: D004768:Enterotoxins; D008565:Membrane Proteins; D011948:Receptors, Antigen, T-Cell; C054979:antigen T cell receptor, zeta chain; C031818:enterotoxin B, staphylococcal