Stimulation of cells using EGF-coated magnetic beads.
The ability to determine the spatial localization of signals can assist in defining the regulation of specific signaling pathways within the cell. Localized application of a stimulus, followed by imaging of various proteins or their phosphorylated products, allows visualization of the propagation scales of responses to the stimulus. To obtain a localized stimulus of epidermal growth factor (EGF), we bound the ligand to magnetic beads, which could be rapidly pulled onto cell surfaces by a magnet. This method allows the spatial analysis of EGF-induced signaling pathways.
Kempiak
Stephan J
SJ
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, New York, NY 10461, USA.
Segall
Jeffrey E
JE
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D045744:Cell Line, Tumor; D002633:Chemotaxis / Q000187:drug effects / Q000502:physiology; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D008280:Magnetics; D015674:Mammary Neoplasms, Animal / Q000378:metabolism* / Q000473:pathology; D008863:Microspheres; D051381:Rats; D012107:Research Design; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology
D004815:Epidermal Growth Factor