Class 1 integrons increase trimethoprim-sulfamethoxazole MICs against epidemiologically unrelated Stenotrophomonas maltophilia isolates.
Twenty-five plasmid-specified antimicrobial resistance determinants common to gram-negative bacilli from nosocomial infection were investigated from 31 Stenotrophomonas maltophilia isolates. Twenty-four clones were identified by pulsed-field gel electrophoresis, and in three clones that exhibited an increased trimethoprim-sulfamethoxazole MIC, the sul1 determinant was found. These results support not only the higher spread of class 1 integrons compared to other mechanisms but also the potential limitation of using trimethoprim-sulfamethoxazole for therapy of severe S. maltophilia infections.
Barbolla
Raquel
R
Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
Catalano
Mariana
M
Orman
Betina E
BE
Famiglietti
Angela
A
Vay
Carlos
C
Smayevsky
Jorgelina
J
Centrón
Daniela
D
Piñeiro
Silvia A
SA
D003428:Cross Infection / Q000382:microbiology; D005798:Genes, Bacterial / Q000235:genetics; D005838:Genotype; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D032023:Integrons / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D005810:Multigene Family / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020615:Stenotrophomonas maltophilia / Q000187:drug effects* / Q000235:genetics*; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000494:pharmacology*
D015662:Trimethoprim, Sulfamethoxazole Drug Combination