Polyelectrolyte multilayer films with pegylated polypeptides as a new type of anti-microbial protection for biomaterials.
Adhesion of bacteria at the surface of implanted materials is the first step in microbial infection, leading to post-surgical complications. In order to reduce this adhesion, we show that poly(L-lysine)/poly(L-glutamic acid) (PLL/PGA) multilayers ending by several PLL/PGA-g-PEG bilayers can be used, PGA-g-PEG corresponding to PGA grafted by poly(ethylene glycol). Streaming potential and quartz crystal microbalance-dissipation measurements were used to characterize the buildup of these films. The multilayer films terminated by PGA and PGA-g-PEG were found to adsorb an extremely small amount of serum proteins as compared to a bare silica surface but the PGA ending films do not reduce bacterial adhesion. On the other hand, the adhesion of Escherichia coli bacteria is reduced by 72% on films ending by one (PLL/PGA-g-PEG) bilayer and by 92% for films ending by three (PLL/PGA-g-PEG) bilayers compared to bare substrate. Thus, our results show the ability of PGA-g-PEG to be inserted into multilayer films and to drastically reduce both protein adsorption and bacterial adhesion. This kind of anti-adhesive films represents a new and very simple method to coat any type of biomaterials for protection against bacterial adhesion and therefore limiting its pathological consequences.
Boulmedais
F
F
Institut Charles Sadron, UPR 22 CNRS, 6 rue Boussingault, 67083 Strasbourg Cedex, France.
Frisch
B
B
Etienne
O
O
Lavalle
Ph
P
Picart
C
C
Ogier
J
J
Voegel
J-C
JC
Schaaf
P
P
Egles
C
C
D000327:Adsorption; D001422:Bacterial Adhesion / Q000502:physiology; D001798:Blood Proteins / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004573:Electrolytes / Q000737:chemistry; D004926:Escherichia coli / Q000166:cytology* / Q000502:physiology*; D005026:Ethylene Glycols / Q000737:chemistry*; D008422:Materials Testing; D010455:Peptides / Q000737:chemistry; D011099:Polyglutamic Acid / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
D001798:Blood Proteins; D020099:Coated Materials, Biocompatible; D004573:Electrolytes; D005026:Ethylene Glycols; D010455:Peptides; D011108:Polymers; D011099:Polyglutamic Acid