Dermal wound healing processes with curcumin incorporated collagen films.
The wound healing process involves extensive oxidative stress to the system, which generally inhibits tissue remodeling. In the present study, an improvement in the quality of wound healing was attempted by slow delivery of antioxidants like curcumin from collagen, which also acts as a supportive matrix for the regenerative tissue. Curcumin incorporated collagen matrix (CICM) treated groups were compared with control and collagen treated rats. Biochemical parameters and histological analysis revealed that increased wound reduction, enhanced cell proliferation and efficient free radical scavenging in CICM group. The higher shrinkage temperature of CICM films suggests increased hydrothermal stability when compared to normal collagen films. Spectroscopic studies revealed that curcumin was bound to the collagen without affecting its triple helicity. Further we adopted the antioxidant assay using 2,2'-azobisisobutyronitrile to assess in vitro antioxidant activity of CICM. The antioxidant studies indicated that CICM quenches free radicals more efficiently. This study provides a rationale for the topical application of CICM as a feasible and productive approach to support dermal wound healing.
Gopinath
D
D
Bioorganic and Neurochemistry Laboratory, Central Leather Research Institute, Adyar, 600 020, Chennai, India.
Ahmed
M Rafiuddin
MR
Gomathi
K
K
Chitra
K
K
Sehgal
P K
PK
Jayakumar
R
R
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000737:chemistry; D002455:Cell Division; D003094:Collagen / Q000737:chemistry* / Q000627:therapeutic use; D003474:Curcumin / Q000008:administration & dosage* / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000008:administration & dosage; D016503:Drug Delivery Systems; D004355:Drug Stability; D004791:Enzyme Inhibitors / Q000008:administration & dosage; D005240:Feasibility Studies; D015227:Lipid Peroxidation; D008297:Male; D008422:Materials Testing; D008567:Membranes, Artificial; D008968:Molecular Conformation; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects / Q000293:injuries / Q000473:pathology; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*; D014950:Wounds, Penetrating / Q000188:drug therapy* / Q000473:pathology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D004791:Enzyme Inhibitors; D008567:Membranes, Artificial; D003094:Collagen; D003474:Curcumin