The significance of altered gelatinase expression in the synovium of patient with arthritic effusions.
In this study we quantified the levels of matrix metalloproteinase-2 and 9 (MMP-2 and 9) in effusions and serial synovial cultures of patients with arthritis of the knee in order to investigate the correlations between MMP and cell counts in effusions as well as the possible roles of the synovium. In 49 patients with arthritis of the knee (series I) we examined the cell counts and the amounts of MMP-2 and 9 in 51 effusions. In 20 knee samples of series I of patients who received arthroscopy (series II), we examined the amounts of MMP-2 and 9 in effusions and serial synovial organ cultures. We also compared the gene expressions of MMP-2 and 9 and MT1-MMP in serial synovial cultures using RT-PCR. In series I, significantly more proMMP-9 appeared in effusions from the inflammatory group than in the non-inflammatory and hemorrhagic group ( p <0.001). The levels of proMMP-9 correlated with the neutrophil counts in the effusions ( p <0.001). In series II synovial cultures, the activities of latent and activated forms of MMP-2 and 9 in lesional areas were all higher than that in paralesional ones ( p <0.05). In RT-PCR analysis, MMP-2, -9 and membrane type 1 MMP mRNA levels of lesional areas also showed increased expression. Our data suggest that the analysis of MMP-9 indicates the inflammatory condition of the joints and that additional synovectomy may be beneficial for patients with inflammatory synovitis, compared with non-inflammatory and hemorrhagic arthritis.
Lu
Ko-Hsiu
KH
Department of Orthopaedic Surgery, Chung Shan Medical University Hospital, 402, Taichung, Taiwan.
Yang
Shun-Fa
SF
Chu
Shu-Chen
SC
Chen
Pei-Ni
PN
Chou
Ming-Chih
MC
Hsiao
Tang-Yan
TY
Hsieh
Yih-Shou
YS
D000368:Aged; D000369:Aged, 80 and over; D001168:Arthritis / Q000201:enzymology* / Q000473:pathology; D001182:Arthroscopy; D002452:Cell Count; D002478:Cells, Cultured; D004789:Enzyme Activation; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007719:Knee Joint / Q000000981:diagnostic imaging / Q000201:enzymology / Q000473:pathology; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis* / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009504:Neutrophils / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D011859:Radiography; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013582:Synovial Fluid / Q000166:cytology / Q000201:enzymology*; D013583:Synovial Membrane / Q000201:enzymology* / Q000473:pathology
D012333:RNA, Messenger; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9