title: Cytotoxicity and mode of action of maleic hydrazide in root tips of Allium cepa L.
abstract: Maleic hydrazide (MH) is an herbicide and is a regulator of the growth of buds in vegetables during storage. It is used in agriculture-in despite its known effect as a mutagenic and clastogenic agent. In this research the effect of MH on the root tips of Allium cepa L. was determined; correlations between the effects of different concentrations and exposure times on the mitotic index (MI) and induction of chromosomal aberrations (ChA) were also examined. Experiments were carried out in triplicate, using aqueous solutions of MH to concentrations of 10(-6), 10(-5), 10(-4) and 10(-3)M, at intervals of 0, 4, 8, 12, 24, and 48 h, with a control for each combination (with the MH substituted by distilled water). The results revealed an inhibition of the MI linked to the concentration and time of treatment (F=845.51, P<0.01 and F=427.58, P<0.01, respectively). For all the concentrations used and exposure periods longer than 12 h, different types of ChA were present, with significantly increased frequencies with increases in the concentration and time of exposure (P<0.01). To determine the mechanism through which the herbicide exerts its toxicity, ultrastructural electron microscopy was conducted. The results reveal nucleolar alterations, suggesting an inhibitory effect of biosynthetic activity.
authors.affiliation: Urbanización Monte Bello, AV 12 con calle Q No. 12-21, Maracaibo, Estado Zulian, Venezuela. letty@iamnet.com
authors.forename: L
authors.initials: L
authors.lastname: Marcano
authors.forename: I
authors.initials: I
authors.lastname: Carruyo
authors.forename: A
authors.initials: A
authors.lastname: Del Campo
authors.forename: X
authors.initials: X
authors.lastname: Montiel
mesh_terms: D000490:Allium / Q000235:genetics*; D002869:Chromosome Aberrations / Q000187:drug effects*; D004249:DNA Damage; D004781:Environmental Exposure; D006540:Herbicides / Q000494:pharmacology / Q000633:toxicity*; D008300:Maleic Hydrazide / Q000494:pharmacology / Q000633:toxicity*; D008854:Microscopy, Electron; D009152:Mutagenicity Tests; D018517:Plant Roots
chemical_list: D006540:Herbicides; D008300:Maleic Hydrazide