Anti-coagulant rodenticide binding properties of human serum albumin: a biochromatographic approach.
In this paper, the anti-coagulant rodenticide-human serum albumin (HSA) binding was investigated using a perturbation method to calculate the solute distribution isotherms. It was shown that rodenticide can bound either on the benzodiazepine HSA site with low affinity (site I) or on the warfarin HSA site with high affinity (site II). The thermodynamic parameters of this association were calculated for the two HSA binding sites. For the site II, the rodenticide-HSA association was governed enthalpically whereas for the site I, this one was driven entropically. Moreover, the role of the magnesium (Mg(2+)) and calcium (Ca(2+)) on this association was carried out. It was clearly demonstrated that the rodenticide affinity for the site I was not affected by modifying the bulk solvent surface tension whereas for the site II the association constant increased strongly with the Mg(2+) or the Ca(2+) concentration in the bulk solvent. These results showed that the rodenticide-HSA affinity and thus the rodenticide toxicological effect depends on the Mg(2+) or Ca(2+) concentration.
André
Claire
C
Laboratoire de Chimie Analytique, Faculté Médecine Pharmacie, Universite de Franche-Comte, Place Saint-Jacques, 25030 Cedex, Besançon, France.
Guillaume
Yves Claude
YC
D000925:Anticoagulants / Q000378:metabolism*; D001665:Binding Sites; D002118:Calcium / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D008274:Magnesium / Q000494:pharmacology; D012378:Rodenticides / Q000378:metabolism*; D012709:Serum Albumin / Q000378:metabolism*; D013816:Thermodynamics
D000925:Anticoagulants; D007202:Indicators and Reagents; D012378:Rodenticides; D012709:Serum Albumin; D008274:Magnesium; D002118:Calcium