title: Differentially expressed genes in hypertensive rats developing cerebral ischemia.
abstract: The molecular events occurring after cerebral ischemia in hypertension may include de novo expression of numerous genes. Receptor genes are predominantly involved in the process of cell death, neuroprotection and reconstruction after ischemic injury. Ischemic stroke was observed in the non-genetic, non-surgical model of hypertension, the cold-induced hypertensive rat. In hypertensive rats suppression subtractive hybridization analysis was used to identify differentially expressed receptor genes in stroke-tissue compared to normal rat brain. We found 76 genes predominantly expressed in hypertensive rat stroke-tissue. These predominantly expressed genes included genes involved in energy metabolism, signal transduction/cell regulation, and replication/transcription/translation. For example, the T3 receptor alpha was predominantly expressed in stroke-tissue, indicating that regeneration of nerves in stroke tissue may be facilitated by increased T3 receptor alpha expression.
authors.affiliation: Department of Hypertension and Endocrinology, Daping Hospital, Third Military Medical University, Chongqing 400042, PR China. Zhuzm@yahoo.com
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Zhu
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.forename: Xianmei
authors.initials: X
authors.lastname: Wang
authors.forename: Shanjun
authors.initials: S
authors.lastname: Zhu
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Youyuan
authors.initials: Y
authors.lastname: Xu
authors.forename: Rutai
authors.initials: R
authors.lastname: Hui
authors.forename: Martin
authors.initials: M
authors.lastname: Tepel
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002545:Brain Ischemia / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D030541:Databases, Genetic; D005786:Gene Expression Regulation; D006973:Hypertension / Q000235:genetics* / Q000378:metabolism; D012313:RNA / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D011988:Receptors, Thyroid Hormone / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D011988:Receptors, Thyroid Hormone; D012313:RNA