[Study on effects of interleukin-1 and their inhibitors for proliferation and hyaluronic acid synthesis of cultured human orbital fibroblasts].
OBJECTIVE
To observe the proliferation and synthesis of hyaluronic acid (HA) of cultured human orbital fibroblasts (OFs) affected by interleukin-1 (IL-1), and their inhibitors interleukin-1 receptor antagonist(IL-1Ra).


METHODS
OFs from 5 patients with thyroid-associated ophthalmopathy (TAO) and 4 normal subjects were cultured in vitro. IL-1, IL-1Ra with different concentrations were put into media respectively, and IL-1(500 U/ml), IL-1Ra(5, 50 ng/ml) were put into media together. The proliferation and HA production of OFs were measured by MTT and radioimmunological technique.


RESULTS
IL-1(550,500 U/ml) can significantly increase the proliferation of OFs and synthesis of HA (P < 0.05), IL-1 (500 U/ml) stimulate more proliferation of OFs and HA production of OFs from TAO patients than that of OFs from normal subjects. While IL-1Ra alone had no inhibitive effects at any concentrations. IL1Ra (5.50 ng/ml) can block the proliferation and HA production of OFs when IL-1 with IL-1Ra incubated together.


CONCLUSIONS
IL-1 can stimulate the proliferation and HA synthesis of OFs. IL-1Ra is an effective inhibitor of proliferation and synthesis of OFs induced by IL-1. IL-1Ra may be useful in the prevention or treatment of TAO.
Luo
Qingli
Q
Department of Ophthalmology, West China Hospital of Sichuan University, Chengdu 610041, China.
Zhang
Xuejin
X
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000473:pathology*; D006111:Graves Disease / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000494:pharmacology*; D009915:Orbit / Q000473:pathology; D012795:Sialoglycoproteins / Q000494:pharmacology*
C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D012795:Sialoglycoproteins; D006820:Hyaluronic Acid