title: A technical trick for studying proteomics in parallel to transcriptomics in symbiotic root-fungus interactions.
abstract: We have developed a protocol in which proteins and mRNA can be analyzed from single root samples. This experimental design was validated in arbuscular mycorrhiza by comparing the proteins profiles obtained with those from a classical protein extraction process. It is a step forward to make simultaneous proteome and transcriptiome profiling possible.
authors.affiliation: UMR 1088 INRA/CNRS 5184/U, Bourgogne (Plante-Microbe- Environnement) INRA-CMSE, Dijon, France. dumas@epoisses.inra.fr
authors.forename: Eliane
authors.initials: E
authors.lastname: Dumas-Gaudot
authors.forename: Nardjis
authors.initials: N
authors.lastname: Amiour
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Weidmann
authors.forename: Gwénaëlle
authors.initials: G
authors.lastname: Bestel-Corre
authors.forename: Benoit
authors.initials: B
authors.lastname: Valot
authors.forename: Sébastien
authors.initials: S
authors.lastname: Lenogue
authors.forename: Vivienne
authors.initials: V
authors.lastname: Gianinazzi-Pearson
authors.forename: Silvio
authors.initials: S
authors.lastname: Gianinazzi
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000032:analysis; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D029902:Medicago / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D038821:Mycorrhizae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis*; D013559:Symbiosis / Q000502:physiology
chemical_list: D005656:Fungal Proteins; D020543:Proteome; D012333:RNA, Messenger