[Effects of basic fibroblast growth factor on the matrix metalloproteinase-2,7 and its tissue inhibitor on deep partial thickness burn wounds in rats].
OBJECTIVE
To observe the changes in matrix metalloproteinase-2,7 (MMP-2,7) and tissue inhibitor of metalloproteinase-2 (TIMP-2) in deep partial thickness burn during the process of wound healing, and the effects of bFGF on wound healing.


METHODS
The rats inflicted by 30% TBSA deep partial thickness burn were randomly divided into simple scald and bFGF treatment groups. Biopsies from wound skin were harvested at 3 and 6PBHs and 1, 3, 7, 14 PBDs for the detection of the epithelialization rate and collagen content. The above indices were also detected in the skin of another 6 normal rats as normal control.


RESULTS
(1) The epithelialization rate in bFGF treatment group was higher than that in simple scald group during 3PBH to 14 PBD. (2) The collagen contents in both bFGF treatment group and simple scald group were continually decreased during 3 PBH to 3 PBD, and increased from 7 to 14 PBD, but still lower than that in normal control (P < 0.05). (3) The expression of MMP-2,7 and TIMP-2 in simple scald group enhanced from 1 to 14 PBD, and peaked on 7 PBD. (4) The expression of MMP-2,7 in bFGF treatment group was similar to that in simple scald group from 3 to 6 PBH, while the expressions of MMP-2,7 and TIMP-2 was higher than those in simple scald group from 1 to 14 PBD.


CONCLUSIONS
The collagen deposition would be affected by the activities of extracellular matrix in scald wound in rats. Changes in MMP-2,7 and TIMP-2 expressions were an important process of wound repair, which was closely related to the acceleration of wound healing by the application of bFGF.
Cheng
Biao
B
Burn Institute of the 304th Hospital, PLA, Beijing 100037, P.R. China.
Fu
Xiao-bing
XB
Sheng
Zhi-yong
ZY
Sun
Tong-zhu
TZ
Gu
Xiao-man
XM
Sun
Xiao-qing
XQ
D000818:Animals; D002056:Burns / Q000378:metabolism*; D003094:Collagen / Q000032:analysis; D004848:Epithelium / Q000502:physiology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000032:analysis*; D020783:Matrix Metalloproteinase 7 / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000032:analysis*; D014945:Wound Healing
D016222:Fibroblast Growth Factor 2; D019716:Tissue Inhibitor of Metalloproteinase-2; D003094:Collagen; D020783:Matrix Metalloproteinase 7; D020778:Matrix Metalloproteinase 2