Polyozellin isolated from Polyozellus multiplex induces phase 2 enzymes in mouse hepatoma cells and differentiation in human myeloid leukaemic cell lines.
Induction of cellular phase 2 detoxifying enzymes is associated with cancer preventive potential. Quinone reductase (QR) has been used as a prototype for anticarcinogenic phase 2 enzymes because of its widespread distribution in mammalian systems, large amplitude of inducer response, and ease of measurement in murine hepatoma cells. Methanol extract of Polyozellus multiplex, which shows a strong QR induction potential, was subjected to bioassay-guided fractionation, and polyozellin (PM1) appeared to be a major active component. In in vitro cultured cells (hepa1c1c7 and BPRc1 cells), polyozellin enhanced QR, glutathione S-transferase (GST) activities, and glutathione (GSH) content in a dose-dependent manner. The compound also significantly promoted differentiation of HL-60 human promyelocytic emia cells. In conclusion, polyozellin deserves further in vivo study to evaluate its potential as a cancer preventive agent.
Kim
Jeong Hyun
JH
Department of Animal Science and Biotechnology, Kyungpook National University, Daegu 702-701, Korea.
Lee
Jeong Soon
JS
Song
Kyung-Sik
KS
Kwon
Chong-Suk
CS
Kim
Young Kyoon
YK
Kim
Jong-Sang
JS
D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D016588:Anticarcinogenic Agents; D002454:Cell Differentiation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D005663:Furans / Q000302:isolation & purification / Q000494:pharmacology*; D005978:Glutathione / Q000032:analysis; D005982:Glutathione Transferase / Q000096:biosynthesis; D006801:Humans; D007951:Leukemia, Myeloid / Q000473:pathology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D000432:Methanol; D051379:Mice; D014407:Tumor Cells, Cultured
D016588:Anticarcinogenic Agents; D005663:Furans; C109015:polyozellin; D005982:Glutathione Transferase; D005978:Glutathione; D000432:Methanol