In vitro lesion repair by human amnion epithelial and mesenchymal cells.
OBJECTIVE
The purpose of this study was to compare wound healing by human amnion epithelial and mesenchymal cells from preterm and term placenta with the use of an in vitro lesion repair assay.


METHODS
Lesions were created in confluent monolayers of amnion epithelial and mesenchymal cells from preterm and term placentas. The repair was monitored by the measurement of the lesion area and the response to potential stimulants (platelet-derived growth factor, tumor necrosis factor-alpha, fibrinogen, and phorbol myristate acetate). Cell proliferation was detected with 5-bromodeoxyuridine staining.


RESULTS
Lesion repair was complete within 40 hours in control epithelial cultures from preterm and term placenta but incomplete in mesenchymal cultures (preterm cells, 80%; term cells, 40%). Platelet-derived growth factor, tumor necrosis factor-alpha, fibrinogen, and phorbol myristate acetate did not accelerate repair in either cell type.


CONCLUSIONS
An in vitro lesion repair assay revealed the differences in lesion repair capacity between amnion epithelial and mesenchymal cells and between mesenchymal cells from preterm and term placenta.
Bilic
Grozdana
G
Department of Obstetrics, Unit of Perinatal Physiology, Zurich University Hospital, Switzerland.
Ochsenbein-Kölble
Nicole
N
Hall
Heike
H
Huch
Renate
R
Zimmermann
Roland
R
D000650:Amnion / Q000293:injuries* / Q000503:physiopathology*; D002455:Cell Division; D002478:Cells, Cultured; D004847:Epithelial Cells; D005260:Female; D005340:Fibrinogen / Q000494:pharmacology; D005865:Gestational Age; D006801:Humans; D008648:Mesoderm; D010920:Placenta; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D012421:Rupture / Q000473:pathology / Q000503:physiopathology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects
D010982:Platelet-Derived Growth Factor; D014409:Tumor Necrosis Factor-alpha; D005340:Fibrinogen; D013755:Tetradecanoylphorbol Acetate