Existence of GPR40 functioning in a human breast cancer cell line, MCF-7.
GPR40, which has recently been identified as a G-protein-coupled cell-surface receptor for long-chain fatty acids, was assessed in a human breast cancer cell line (MCF-7). We detected GPR40 mRNA by RT-PCR and found that oleate and linoleate, but not palmitate or stearate, caused an increase in cellular Ca(2+) concentrations, which was partially blocked by the pertussis toxin (PTX) treatment. We examined the expression of GPR40 mRNA by quantitative RT-PCR in the relation to cell number. It was significantly increased at the beginning and at the end of cell proliferation. These results indicate the possibility that GPR40 for long-chain fatty acids may be involved in cellular function such as cell proliferation, providing a new perspective for the action of long-chain fatty acids on mammary epithelial cells.
Yonezawa
T
T
Department of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Amamiyamachi, 981-8555, Sendai, Japan.
Katoh
K
K
Obara
Y
Y
D001943:Breast Neoplasms / Q000378:metabolism*; D002118:Calcium / Q000032:analysis / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D003600:Cytosol / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D018613:Microscopy, Confocal; D037342:Pertussis Toxin / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
C473034:FFAR1 protein, human; D005227:Fatty Acids; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D037342:Pertussis Toxin; D002118:Calcium