Chondroitin sulfate of appican, the proteoglycan form of amyloid precursor protein, produced by C6 glioma cells interacts with heparin-binding neuroregulatory factors.
Appican produced by rat C6 glioma cells, the chondroitin sulfate (CS) proteoglycan form of the amyloid precursor protein, contains an E disaccharide, -GlcUA-GalNAc(4,6-O-disulfate)-, in its CS chain. In this study, the appican CS chain from rat C6 glioma cells was shown to specifically bind several growth/differentiation factors including midkine (MK) and pleiotrophin (PTN). In contrast, the appican CS from SH-SY5Y neuroblastoma cells contained no E disaccharide and showed no binding to either MK or PTN. These findings indicate that the E motif is essential in the interaction of the appican CS chain with growth/differentiation factors, and suggest that glial appican may mediate the regulation of neuronal cell adhesion and migration and/or neurite outgrowth.
Umehara
Yuko
Y
Department of Biochemistry, Kobe Pharmaceutical University, 4-19-1 Motoyamakita-machi, Higashinada-ku, Kobe 658-8558, Japan.
Yamada
Shuhei
S
Nishimura
Shuji
S
Shioi
Junichi
J
Robakis
Nikolaos K
NK
Sugahara
Kazuyuki
K
D016564:Amyloid beta-Protein Precursor; D002240:Carbohydrate Sequence; D002352:Carrier Proteins / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D005910:Glioma; D006025:Glycosaminoglycans / Q000737:chemistry / Q000378:metabolism; D006493:Heparin / Q000378:metabolism; D006801:Humans; D000078792:Midkine; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009447:Neuroblastoma; D009844:Oligosaccharides / Q000737:chemistry; D011509:Proteoglycans / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D016564:Amyloid beta-Protein Precursor; D002352:Carrier Proteins; D016207:Cytokines; D006025:Glycosaminoglycans; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D009844:Oligosaccharides; D011509:Proteoglycans; C093487:appican; C066845:pleiotrophin; D000078792:Midkine; D006493:Heparin