title: Imaging of gliomas with Cis-4-[18F]fluoro-L-proline.
abstract: Tumor imaging with cis-4-[18F]fluoro-L-proline (cis-FPro) was compared to that of L-[3H]proline and L-[3H]methionine in F98 rat gliomas by dual-tracer autoradiography. All tracers exhibited high accumulation in the tumors but in the normal brain significant uptake was observed for L-[3H]methionine only. Tumor extent on autoradiograms with L-[3H]proline and L-[3H]methionine was identical to that of histological staining while autoradiograms of cis-FPro showed diffuse uptake in the penumbra of some tumors. First PET studies in 7 patients with cerebral gliomas demonstrated accumulation of cis-FPro in tumor areas with enhancement of Gd-DTPA on MR scans. Uptake of cis-FPro in normal brain tissue was negligible. In one patient with a glioblastoma accumulation of cis-FPro was also found in two brain areas without enhancement of Gd-DTPA on MR scans. Control of MRI suggested tumor growth in these areas at further follow up. Our results indicate that in most gliomas increased cis-FPro uptake is restricted to areas with disruption of the BBB which limits its clinical utility.
authors.affiliation: Institute of Medicine, Research Centre Jülich, Jülich, Germany. k.j.langen@fz-juelich.de
authors.forename: Karl J
authors.initials: KJ
authors.lastname: Langen
authors.forename: Michael
authors.initials: M
authors.lastname: Jarosch
authors.forename: Kurt
authors.initials: K
authors.lastname: Hamacher
authors.forename: Heinz
authors.initials: H
authors.lastname: Mühlensiepen
authors.forename: Friedrich
authors.initials: F
authors.lastname: Weber
authors.forename: Frank
authors.initials: F
authors.lastname: Floeth
authors.forename: Dirk
authors.initials: D
authors.lastname: Pauleit
authors.forename: Hans
authors.initials: H
authors.lastname: Herzog
authors.forename: Heinz H
authors.initials: HH
authors.lastname: Coenen
mesh_terms: D000328:Adult; D000818:Animals; D001345:Autoradiography; D001932:Brain Neoplasms / Q000175:diagnosis / Q000000981:diagnostic imaging*; D045744:Cell Line, Tumor; D005260:Female; D005910:Glioma / Q000175:diagnosis / Q000000981:diagnostic imaging*; D006801:Humans; D008297:Male; D008715:Methionine / Q000031:analogs & derivatives*; D008875:Middle Aged; D011392:Proline / Q000031:analogs & derivatives; D019275:Radiopharmaceuticals; D051381:Rats; D011916:Rats, Inbred F344; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014055:Tomography, Emission-Computed / Q000379:methods*
chemical_list: D019275:Radiopharmaceuticals; C431803:fluoro-proline; D011392:Proline; D008715:Methionine; C038344:methionine methyl ester