Presurgical antimicrobial prophylaxis: effect on ocular flora in healthy patients.
OBJECTIVE
Evaluation of presurgical antimicrobial prophylaxis for reduction of ocular flora.


METHODS
Ophthalmology Section, Department of Medicine and Surgery, University of Catania, Italy.


METHODS
Three days before photorefractive keratectomy (PRK), conjunctiva of 70 healthy patients (100 eyes) were swabbed. After 3 days of instillation of ofloxacin 0.3% (3 times daily), conjunctival swabs were taken again. Bacteria were isolated and identified from each swab.


RESULTS
A total of 191 independent isolates were obtained prior to antimicrobial treatment. Bacteria were isolated from all 100 eyes sampled. Gram-positive species predominated, with Staphylococcus epidermidis and Staphylococcus aureus cultured from 69% and 25% of eyes sampled, respectively. Species of the anaerobic genera Peptococcus and Peptostreptococcus were found in 22% and 14% of eyes, respectively. After prophylaxis with ofloxacin, bacteria could be cultured from only 7% of eyes (10 independent isolates). All isolates were sensitive to ofloxacin except 5 strains of S. epidermidis, which displayed intermediate sensitivity. No infections occurred after PRK.


CONCLUSIONS
Prophylaxis by instillation of ofloxacin 0.3% 3 times daily for 3 days substantially reduced the ocular flora of 100 healthy eyes prior to refractive surgery.
Chisari
G
G
Center for Ocular Microbiology, Ophthalmology Section, University of Catania, Italy. GChisari@UNICT.it
Cavallaro
G
G
Reibaldi
M
M
Biondi
S
S
D000287:Administration, Topical; D000328:Adult; D019072:Antibiotic Prophylaxis / Q000379:methods*; D015169:Colony Count, Microbial; D003470:Culture Media; D004334:Drug Administration Schedule; D024881:Drug Resistance, Bacterial; D005123:Eye / Q000187:drug effects* / Q000382:microbiology*; D005260:Female; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000187:drug effects / Q000302:isolation & purification; D006801:Humans; D007558:Italy; D054018:Lasers, Excimer; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D015242:Ofloxacin / Q000008:administration & dosage / Q000493:pharmacokinetics; D009883:Ophthalmic Solutions; D018903:Photorefractive Keratectomy / Q000379:methods; D011300:Preoperative Care / Q000379:methods*
D003470:Culture Media; D009883:Ophthalmic Solutions; D015242:Ofloxacin