[Effect of handportable mobiletelephone microwave radiation on rat central neuron apoptosis].
OBJECTIVE
To investigate the effect of handportable mobiletelephone microwave radiation on rat central nervous system by setting up rat model.


METHODS
80 healthy male SD rats (weighed about 200 g) were divided into 4 groups at random: control, radiation, decranium, decranium + radiation. TUNEL method was adopted used to detect the apoptosis of neurons after irradiation, then immunohistochemistry was used to detect Bcl-2, Bax expression in all brain tissue.


RESULTS
TUNEL positive rate, Bax and Bcl-2 positive cell numbers could be found in decranium + radiation group [(26.45 +/- 9.27)%, (23.5 +/- 3.58), (11.1 +/- 2.55) respectively]. There were significant differences among control [(9.59 +/- 2.55)%, 14.2 +/- 2.46, 7.0 +/- 1.14 respectively], decranium group [(9.52 +/- 1.93)%, 15.5 +/- 1.77, 7.4 +/- 1.76], radiation group [(10.04 +/- 3.62)%, 15.9 +/- 2.02, 7.2 +/- 1.07] (P < 0.01). But the difference was not found in the ratio of Bax to Bcl-2.


CONCLUSIONS
Microwave radiation did not affect the intact rat, but did promote the occurrence of neuron apoptosis in cranial defect rat. Bax, Bcl-2 gene participated in regulation of apoptosis. The intact cranium may be an important factor to protect the neurons against handportable mobiletelephone microwave radiation to some extent.
Liu
Wei-guo
WG
The Second Hospital, College of Medical Science, Zhejiang University, Hangzhou 310009, China.
Yang
Xiao-feng
XF
Zhu
Yong-jian
YJ
Shen
Hong
H
Jiang
Xiu-yi
XY
Lu
Shi-ting
ST
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects*; D001921:Brain / Q000473:pathology / Q000528:radiation effects*; D020287:In Situ Nick-End Labeling; D008297:Male; D008872:Microwaves / Q000009:adverse effects*; D009474:Neurons / Q000473:pathology / Q000528:radiation effects*; D011518:Proto-Oncogene Proteins / Q000032:analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D051028:bcl-2-Associated X Protein
C494828:Bax protein, rat; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein