Antioxidant supplementation preserves antioxidant response in physical training and low antioxidant intake.
The present controlled-training double-blind study (supplemented (S) group, n 7; placebo (P) group, n 10) was designed to investigate whether an antioxidant mixture (Se 150 microg, retinyl acetate mg, ascorbic acid 120 mg, alpha-tocopheryl succinate 20 mg) would allow overloaded triathletes to avoid adaptation failure in the antioxidant system [corrected]. Dietary intakes were recorded. The supplement of Se, and vitamins A and E provided 100 % of the French RDA. Four weeks of overloaded training (OT) followed 4 weeks of normal training (NT). After NT and OT, biological studies were conducted at rest and after a duathlon test (run 5 km, cycle 20 km, run 5 km). During the 4-week period of NT, blood levels of GSH levels increased in response to supplementation (P<0.05) and remained elevated during OT. Plasma glutathione peroxidase activity was significantly higher in the S group in all situations after NT and OT (P<0.01). The S group had increased erythrocyte Cu,Zn-superoxide dismutase activity in response to OT (P<0.05). Supplementation significantly reduced (P<0.05) the magnitude in duathlon-induced creatine kinase isoenzyme MB mass increase, which tended to be higher with OT (P=0.09). We conclude that the antioxidant mixture helped to preserve the antioxidant system during an OT-induced stress in subjects with initially low antioxidant intakes. Effects of supplementation during NT and/or OT are shown mostly by the alleviated muscle damage. The effects of the antioxidant mixture were observed for doses that can be provided by a diversified and well-balanced diet. The maintenance of normal nutritional status with regard to the antioxidant intake (Se, vitamins C and E) plays a key role in antioxidant adaptive effects during NT and OT.
Palazzetti
Stéphane
S
Laboratoire Physiologie des Adaptations, Performance Motrice et Santé, Université de Nice-Sophia-Antipolis, France.
Rousseau
Anne-Sophie
AS
Richard
Marie-Jeanne
MJ
Favier
Alain
A
Margaritis
Irène
I
D000222:Adaptation, Physiological / Q000502:physiology; D000273:Adipose Tissue / Q000378:metabolism; D000328:Adult; D000975:Antioxidants / Q000008:administration & dosage* / Q000378:metabolism; D015992:Body Mass Index; D019587:Dietary Supplements; D004311:Double-Blind Method; D004734:Energy Metabolism; D006801:Humans; D008297:Male; D009752:Nutritional Status / Q000502:physiology; D018384:Oxidative Stress / Q000502:physiology; D010809:Physical Fitness; D012643:Selenium / Q000008:administration & dosage / Q000378:metabolism
D000975:Antioxidants; D012643:Selenium