Multiplexed toxin analysis using four colors of quantum dot fluororeagents.
Quantum dots (QDs) have the potential to simplify the performance of multiplexed analysis. In this work, we prepared bioinorganic conjugates made with highly luminescent semiconductor nanocrystals (CdSe-ZnS core-shell QDs) and antibodies to perform multiplexed fluoroimmunoassays. Sandwich immunoassays for the detection of cholera toxin, ricin, shiga-like toxin 1, and staphylococcal enterotoxin B were performed simultaneously in single wells of a microtiter plate. Initially the assay performance for the detection of each toxin was examined. We then demonstrated the simultaneous detection of the four toxins from a single sample probed with a mixture of all four QD-antibody reagents. Using a simple linear equation-based algorithm, it was possible to deconvolute the signal from mixed toxin samples, which allowed quantitation of all four toxins simultaneously.
Goldman
Ellen R
ER
Center for Bio/Molecular Science and Engineering and Division of Optical Sciences, U.S. Naval Research Laboratory, Washington, D.C. 20375, USA. erg@cbmse.nrl.navy.mil
Clapp
Aaron R
AR
Anderson
George P
GP
Uyeda
H Tetsuo
HT
Mauro
J Matthew
JM
Medintz
Igor L
IL
Mattoussi
Hedi
H
D002104:Cadmium / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D005470:Fluorometry / Q000379:methods; D018796:Immunoconjugates / Q000737:chemistry; D007124:Immunoenzyme Techniques; D008163:Luminescent Measurements; D008954:Models, Biological; D015202:Protein Engineering / Q000379:methods; D045663:Quantum Dots; D012643:Selenium / Q000737:chemistry; D012666:Semiconductors; D013440:Sulfides / Q000737:chemistry; D014118:Toxins, Biological / Q000032:analysis* / Q000737:chemistry; D017967:Zinc Compounds / Q000737:chemistry
D018796:Immunoconjugates; D013440:Sulfides; D014118:Toxins, Biological; D017967:Zinc Compounds; D002104:Cadmium; D012643:Selenium; C031238:zinc sulfide