[Effect of low-intensity microwave radiation on proliferation of cultured epithelial cells of rabbit lens].
OBJECTIVE
To compare the effects of different doses of microwave on the proliferative activity and cell cycle of cultured epithelial cells of rabbit lens, and to investigate the limit tolerant of microwave exposure.


METHODS
Cultured epithelial cells of rabbit lens were exposed to microwave radiation with frequency of 2,450 MHz and power density of 0.10, 0.25, 0.50, 1.00, 2.00 mW/cm(2) for 8 h in vitro. HE staining was used to observe the morphological changes of lens epithelial cells, the proliferative activity and cell cycle were measured by MTT assay and PI fluorescent staining.


RESULTS
8 h after radiation, 0.50, 1.00 and 2.00 mW/cm(2) microwave could decrease the proliferation of lens epithelial cells, make the cells disordered arrangement, shrinkage, detachment, and inhibit the synthesis of cell DNA. The percentage of G(0)/G(1) phase cells were 71.95% +/- 2.12%, 75.68% +/- 3.35% and 82.40% +/- 8.68% respectively, which were higher than that in control group (61.68% +/- 5.76%, P < 0.05 or P < 0.01). The percentage of S phase cells were 19.32% +/- 3.07%, 16.08% +/- 4.91% and 12.98% +/- 8.08% respectively, which were lower than that in control group (28.05% +/- 5.12%, P < 0.05 or P < 0.01). No obvious changes could be detected in 0.10, 0.25 mW/cm(2) microwave groups (P > 0.05).


CONCLUSIONS
Microwave exceeding 0.50 mW/cm(2) may make injury to lens epithelial cells after 8 hour radiation, which may be related to the effect of microwave radiation on cell cycle.
Wang
Kai-jun
KJ
Department of Ophthalmology, The Second Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310009, China.
Yao
Ke
K
Lu
De-qiang
DQ
Jiang
Huai
H
Tan
Jian
J
Xu
Wen
W
D000818:Animals; D002453:Cell Cycle / Q000528:radiation effects*; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D007908:Lens, Crystalline / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D008872:Microwaves; D011817:Rabbits
D004247:DNA