title: PDX1, a cellular homeoprotein, binds to and regulates the activity of human cytomegalovirus immediate early promoter.
abstract: Cellular homeoproteins have been shown to regulate the transcription of several viruses, including herpes simplex viruses, human papillomaviruses, and mouse mammary tumor viruses. Previous studies investigating the anti-viral mechanisms of several cyclin-dependent kinase inhibitors showed that the homeoproteins, pre B-cell leukemia transcription factor 1 (PBX1) and PBX-regulating protein-1 (PREP1), function as transcriptional activators of Moloney murine leukemia virus. Here, we examined the involvement of cellular homeoproteins in regulating the activity of the human cytomegalovirus immediate early (CMV IE) promoter. We identified a 45-bp element located at position -593 to -549 upstream of the transcription start site of the CMV IE gene, which contains multiple putative homeoprotein binding motifs. Gel shift assays demonstrated the physical association between a homeodomain protein, pancreatic-duodenal homeobox factor-1 (PDX1) and the 45-bp cytomegalovirus (CMV) region. We further determined that PDX1 represses the CMV IE promoter activity in 293 cells. Overexpression of PDX1 resulted in a decrease in transcription of the CMV IE gene. Conversely, blocking PDX1 protein synthesis and mutating the PDX1 binding sites enhanced CMV IE-dependent transcription. Collectively, our results represent the first work demonstrating that a cellular homeoprotein, PDX1, may be a repressor involved in regulation of human CMV gene expression.
authors.affiliation: Genomics Institute of the Novartis Research Foundation, San Diego, California 92121, USA.
authors.forename: Sheng-Hao
authors.initials: SH
authors.lastname: Chao
authors.forename: Josephine N
authors.initials: JN
authors.lastname: Harada
authors.forename: Francie
authors.initials: F
authors.lastname: Hyndman
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Gao
authors.forename: Christian G
authors.initials: CG
authors.lastname: Nelson
authors.forename: Sumit K
authors.initials: SK
authors.lastname: Chanda
authors.forename: Jeremy S
authors.initials: JS
authors.lastname: Caldwell
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: D000956:Antigens, Viral; D018398:Homeodomain Proteins; D017874:Immediate-Early Proteins; C106682:PKNOX1 protein, human; C491412:Pknox1 protein, mouse; D015534:Trans-Activators; C044582:immediate-early proteins, cytomegalovirus; C084010:pancreatic and duodenal homeobox 1 protein