PCR-RFLP to identify Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis causing American cutaneous leishmaniasis.
A PCR-RFLP based method was developed to diagnose and identify the Leishmania species causing American cutaneous leishmaniasis (ACL) in a panel of clinical samples obtained from an endemic region of Brazil. The comparison of the results obtained by PCR-RFLP and PCR-hybridization in the identification of Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis were highly concordant (kappa=91.5%). The PCR-RFLP method was reliable, fast and easy to conduct on biopsies and presents potential value of utmost importance for the diagnosis and identification of Leishmania in clinical specimens, infected reservoirs and vectors.
Volpini
Angela C
AC
Lab. de Parasitologia Celular e Molecular, Centro de Pesquisas René Rachou-FIOCRUZ; Av. Augusto de Lima 1715, Barro Preto, CEP 30190-002, MG, Belo Horizonte, Brazil.
Passos
Valéria M A
VM
Oliveira
Guilherme Correa
GC
Romanha
Alvaro J
AJ
D000818:Animals; D001483:Base Sequence; D006801:Humans; D007891:Leishmania / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D007892:Leishmania braziliensis / Q000201:enzymology / Q000235:genetics*; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis / Q000469:parasitology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity