Binding of Bifidobacterium bifidum and Lactobacillus reuteri to the carbohydrate moieties of intestinal glycolipids recognized by peanut agglutinin.
We examined binding of Bifidobacterium bifidum and Lactobacillus reuteri to the carbohydrate moieties of glycolipids extracted from human enterocyte-like Caco-2 cells in this study. In binding assays to reference glycolipids of different carbohydrate compositions, B. bifidum EB102 bound strongly to gangliotetraosylceramide (asialo-GM1) and less strongly to gangliotriaosylceramide (asialo-GM2), lactosylceramide and sulfatide. The binding profile of B. bifidum EB102 was almost identical to that of L. reuteri JCM1081 described previously [Lett. Appl. Microbiol. 27 (1998) 130]. When we examined binding to neutral glycolipids extracted from Caco-2 cells, the binding profiles of B. bifidum EB102 and L. reuteri JCM1081 were very similar to that shown by peanut agglutinin (PNA). Binding of both strains to periodate-treated intestinal glycolipids was completely abolished, suggesting that the bacterial cells bind to carbohydrate moieties of the glycolipids. Furthermore, B. bifidum EB102 was found to express multiple glycolipid-binding proteinaceaous components on the cell surface. These results strongly suggested involvement of cell-surface proteinaceous components of B. bifidum in binding to the carbohydrate moieties of intestinal glycolipids recognized by PNA. Binding ability of B. bifidum and L. reuteri to intestinal glycolipids may play a crucial role for colonization on the mucosal surface of the intestine.
Mukai
Takao
T
School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan. mukai@vmas.kitasato-u.ac.jp
Kaneko
Shigenobu
S
Matsumoto
Mitsuyo
M
Ohori
Hitoshi
H
D001644:Bifidobacterium / Q000378:metabolism* / Q000502:physiology; D018938:Caco-2 Cells / Q000382:microbiology*; D050260:Carbohydrate Metabolism; D005677:G(M1) Ganglioside; D005732:Gangliosides; D006017:Glycolipids / Q000378:metabolism*; D006028:Glycosphingolipids; D006801:Humans; D007778:Lactobacillus / Q000378:metabolism* / Q000502:physiology; D019887:Peanut Agglutinin
D005732:Gangliosides; D006017:Glycolipids; D006028:Glycosphingolipids; D019887:Peanut Agglutinin; C038244:ganglio-N-triaosylceramide; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside