Natriuretic peptides inhibit adenylyl cyclase activity in dispersed eel gill cells.
The effect of natriuretic peptides on forskolin-evoked adenylyl cyclase activity was investigated in dispersed gill cells from the Australian short-finned eel (Anguilla australis). Molecular cloning techniques were employed to identify the putative G-protein-activating motif within the intracellular domain of the eel natriuretic peptide C receptor. Eel ANP, eel CNP and the NPR-C-specific C-ANF inhibited the forskolin-stimulated production of cyclic AMP. This effect was abolished by pretreatment of cells with pertussis toxin. Eel VNP was without effect on adenylyl cyclase activity. PCR and molecular cloning indicated that the intracellular domain of A. australis NPR-C has the same amino acid sequence as Anguilla japonica. Alignment of these sequences with Rattus norvegicus NPR-C indicated conservation of the putative G-protein-activating motif BB...BBXXB (B = basic, X = nonbasic residues). These data suggest that branchially-expressed NPR-C may play a physiological role additional to that of ligand clearance.
Callahan
W
W
School of Biological and Chemical Sciences, Deakin University, Vic 3217, 3217, Geelong, Vic, Australia.
Nankervis
S
S
Toop
T
T
D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000595:Amino Acid Sequence; D000811:Anguilla / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology / Q000502:physiology; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D005576:Colforsin / Q000494:pharmacology; D005880:Gills / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006162:Guanylate Cyclase / Q000378:metabolism / Q000502:physiology; D008969:Molecular Sequence Data; D020098:Natriuretic Peptide, C-Type / Q000494:pharmacology / Q000502:physiology; D045265:Natriuretic Peptides / Q000494:pharmacology* / Q000502:physiology; D010446:Peptide Fragments / Q000494:pharmacology / Q000502:physiology; D037342:Pertussis Toxin / Q000494:pharmacology; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D017461:Receptors, Atrial Natriuretic Factor / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D000067956:Adenylyl Cyclase Inhibitors; D045265:Natriuretic Peptides; D010446:Peptide Fragments; C065367:atrial natriuretic factor (4-23)NH2, de-Gln(18)-de-Ser(19)-de-Gly(20,22)-de-Leu(21)-; D020098:Natriuretic Peptide, C-Type; C068986:ventricular natriuretic peptide, eel; D005576:Colforsin; D012313:RNA; D009320:Atrial Natriuretic Factor; D037342:Pertussis Toxin; D000262:Adenylyl Cyclases; D006162:Guanylate Cyclase; D017461:Receptors, Atrial Natriuretic Factor; C073616:atrial natriuretic factor receptor C