title: Importance of integrin beta1-mediated cell adhesion on biodegradable polymers under serum depletion in mesenchymal stem cells and chondrocytes.
abstract: To evaluate the predominant mechanism of chondrogenic cell [mesenchymal stem cells (MSCs) and chondrocytes] adhesion under serum free conditions, we measured the surface roughness and wettability of poly(lactic acid:polyglycolic acid=75:25) (PLGA), poly(lactic acid) (PLA), and poly(-epsilon-caprolactone) (PCL)-coated glass plates. Also to evaluate the biological reactions involved in cell-polymer interactions, integrin beta1, one of the cell adhesion molecules, was blocked with monoclonal antibody. In cell attachment test, MSCs and chondrocytes adhesion to synthetic polymers in 1h were very low and ranged from 2.8% to 8.0%. In present study, the correlation between attachment rate and surface roughness, contact angle, or integrin beta1 blocking on PLGA, PLA and PCL-coated plates could not be proved. However, we found that L-arginine-coated PLA highly increased the attachment rates of MSCs (30.2%) and of chondrocytes (26%), whereas integrin beta1 blocking significantly decreased these attachment rates to 5.6% and 7.4%, respectively, suggesting that increased cell adhesion to L-arginine-coated plates is mediated by integrin beta1. In this study, we showed that polymer characteristics such as roughness and wettability did not play an important role in cell adhesion under serum free conditions, because there was no significant difference according to polymer characteristics, whereas biological interactions mediated by integrin beta1 were critical during the early period of cell adhesion. The results suggest that L-arginine could be useful for facilitating early cell adhesion to synthetic polymers in cartilage tissue engineering.
authors.affiliation: Department of Orthopaedic Surgery, Yonsei University College of Medicine, 120-752, Seoul, Republic of Korea. ljwos@yumc.yonsei.ac.kr
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Lee
authors.forename: Yun Hee
authors.initials: YH
authors.lastname: Kim
authors.forename: Ki Dong
authors.initials: KD
authors.lastname: Park
authors.forename: Kyoung Soo
authors.initials: KS
authors.lastname: Jee
authors.forename: Jung Woog
authors.initials: JW
authors.lastname: Shin
authors.forename: Soo Bong
authors.initials: SB
authors.lastname: Hahn
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000502:physiology*; D016895:Culture Media, Serum-Free; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism*; D019344:Lactic Acid / Q000737:chemistry; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D011091:Polyesters / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties; D013552:Swine; D023822:Tissue Engineering / Q000379:methods; D017155:Wettability
chemical_list: D001672:Biocompatible Materials; D016895:Culture Media, Serum-Free; D019012:Integrin beta1; D011091:Polyesters; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; C016240:polycaprolactone; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide); D001120:Arginine