Acceleration of callus maturation using rhOP-1 in mandibular distraction osteogenesis in a rat model.
The aim of this study was to assess if the application of rhOP-1 induces accelerated consolidation of the callus in mandibular distraction osteogenesis. In seven adult Wistar rats a bilateral osteotomy of the horizontal ramus of the mandible was performed in the molar region and a custom designed distractor was mounted to the mandible. With a rate of 0.7 mm per day the device was activated bilaterally after the seventh postoperative day. After seven days of distraction two times 50 microg rhOP-1 were injected on two subsequent days directly into the callus. The contralateral side received an injection of placebo solution. The animals were killed four weeks after the end of distraction. A three-point bending test revealed a significantly higher strength of the distracted mandible in the rhOP-1 side (66.3 N vs. 30.4 N, P=0.034, paired t-test). Undecalcified histological sections were examined using microradiography and fluorescence microscopy after sequential intravital polychromic labelling. A continuous bony bridging was seen in all rhOP-1 sites and in none of the control sites. The data indicate that rhOP-1 may be an option to accelerate callus maturation in mandibular distraction osteogenesis.
Terheyden
H
H
Department of Oral and Maxillofacial Surgery, University of Kiel, Arnold Heller Str. 16, 24105 Kiel, Germany. terheyden@mkg.uni-kiel.de
Wang
H
H
Warnke
P H
PH
Springer
I
I
Erxleben
A
A
Ludwig
K
K
Rueger
D C
DC
D000818:Animals; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000502:physiology*; D001861:Bone Regeneration / Q000502:physiology*; D002146:Bony Callus / Q000033:anatomy & histology / Q000000981:diagnostic imaging / Q000502:physiology*; D008297:Male; D008334:Mandible / Q000601:surgery; D019186:Mandibular Advancement / Q000379:methods; D010012:Osteogenesis / Q000502:physiology*; D019857:Osteogenesis, Distraction / Q000379:methods*; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D014945:Wound Healing / Q000502:physiology
C525821:Bmp7 protein, rat; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta