A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay.
Reliable quantitative evaluation of molecular pathways is critical for both drug discovery and treatment monitoring. We have modified the CAM assay to quantitatively measure vascular density, endothelial proliferation, and changes in protein expression in response to anti-angiogenic and pro-angiogenic agents. This improved CAM assay can correlate changes in vascular density with changes seen on a molecular level. We expect that these described modifications will result in a single in vivo assay system, which will improve the ability to investigate molecular mechanisms underlying the angiogenic response.
Miller
Walter J
WJ
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA. Steven_Libutti@nih.gov
Kayton
Mark L
ML
Patton
Angela
A
O'Connor
Sarah
S
He
Mei
M
Vu
Huan
H
Baibakov
Galina
G
Lorang
Dominique
D
Knezevic
Vladimir
V
Kohn
Elise
E
Alexander
H Richard
HR
Stirling
David
D
Payvandi
Faribourz
F
Muller
George W
GW
Libutti
Steven K
SK