Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus bataviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A grasslands.
A group of 42 isolates were isolated from the soil of several disused hay fields, in the Drentse A agricultural research area (The Netherlands), that were taken out of production at different times. The group represents hitherto-uncultured Bacillus lineages that have previously been found, by a non-cultural method, to be predominant in soil. The strains were subjected to a polyphasic taxonomic study, including (GTG)5-PCR, 16S rDNA sequence analysis, DNA-DNA hybridizations, DNA base-ratio determination, fatty acid analysis and morphological and biochemical characterization. By comparing the groupings obtained by (GTG)5-PCR and 16S rDNA sequence analysis, six clusters of similar strains could be recognized. A DNA-DNA relatedness study showed that these clusters represented five novel genospecies. Further analysis supported the proposal of five novel species in the genus Bacillus, namely Bacillus novalis sp. nov. (type strain IDA3307T=R-15439T=LMG 21837T=DSM 15603T), Bacillus vireti sp. nov. (type strain IDA3632T=R-15447T=LMG 21834T=DSM 15602T), Bacillus soli sp. nov. (type strain IDA0086T=R-16300T=LMG 21838T=DSM 15604T), Bacillus bataviensis sp. nov. (type strain IDA1115T=R-16315T=LMG 21833T=DSM 15601T) and Bacillus drentensis sp. nov. (type strain IDA1967T=R-16337T=LMG 21831T=DSM 15600T).
Heyrman
Jeroen
J
Ghent University, Department BFM (WE10V), Laboratory of Microbiology, K.-L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Vanparys
Bram
B
Ghent University, Department BFM (WE10V), Laboratory of Microbiology, K.-L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Logan
Niall A
NA
School of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 0BA, UK.
Balcaen
An
A
Ghent University, Department BFM (WE10V), Laboratory of Microbiology, K.-L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Rodríguez-Díaz
Marina
M
School of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 0BA, UK.
Felske
Andreas
A
GBF (German Research Centre for Biotechnology), Division of Microbiology, Mascheroder Weg 1, D-38124 Braunschweig, Germany.
De Vos
Paul
P
Ghent University, Department BFM (WE10V), Laboratory of Microbiology, K.-L. Ledeganckstraat 35, B-9000 Gent, Belgium.
D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002980:Climate; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005858:Germany; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D006109:Poaceae; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S