Potentiation of mitogenesis in adult rat chromaffin cell cultures by immunosuppressive agent FK506.
The immunosuppressive drugs FK506 and cyclosporin inhibit T- and B-lymphocyte proliferation and exert neuritogenic and/or cytoprotective effects on several types of neurons. While the immunosuppressive actions of both drugs are mediated in large part by inhibition of the Ca(2+)-dependent phosphatase, calcineurin, FK506 is known to exert additional effects. In the present study, FK506 is shown to potentiate mitogenic effects of the neurotrophic factor, neurturin, on normal adult rat adrenal chromaffin cells in culture. The effect is not seen with cyclosporin or with a non-immunosuppressive analog of FK506, GPI-1046. The finding of increased mitogenesis in response to FK506 may have applications to the study of normal and neoplastic neuroendocrine cells and to understanding the development of some types of tumors in transplant patients.
Powers
J F
JF
Department of Pathology, Tufts New England Medical Center and Tufts University School of Medicine, Boston, MA 02111, USA. jpowers1@tufts-nemc.org
Brachold
J M
JM
Schelling
K
K
Tischler
A S
AS
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D018929:Cell Culture Techniques; D019439:Chromaffin Cells / Q000166:cytology* / Q000187:drug effects*; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008934:Mitogens / Q000494:pharmacology*; D008938:Mitosis / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D011916:Rats, Inbred F344
D000276:Adjuvants, Immunologic; D007166:Immunosuppressive Agents; D008934:Mitogens