Maternal selenium deficiency increases hydrogen peroxide and total lipid peroxides in porcine fetal liver.
To investigate the role of selenium (Se) in the developing porcine fetus, prepubertal gilts (n = 42) were randomly assigned to either Se-adequate (0.39 ppm Se) or Se-deficient (0.05 ppm Se) gestation diets 6 wk prior to breeding. Maternal and fetal liver was collected at d 30, 45, 70, 90, and 114 of pregnancy. Concentrations of Se in maternal liver decreased during gestation in gilts fed the low-Se diet. The activity of cellular glutathione peroxidase (GPx) was decreased at d 30 and 45 of gestation in liver of gilts fed the low-Se diet. Concentrations of malondialdehyde (MDA) and hydrogen peroxide (H2O2) were greater in liver homogenates from gilts fed the low-Se diet. Within the fetuses, liver Se decreased in those fetuses of gilts fed the low-Se diet. Although the activity of GPx in fetal liver was not affected by the maternal diet, concentrations of H2O2 and MDA in fetal liver were greater in fetuses from gilts fed the low-Se diet. Maternal liver GPx activity was approx 12-fold greater than fetal liver GPx activity regardless of dietary treatment. These results indicate that maternal dietary Se intake affects fetal liver Se concentration and feeding a low-Se diet during gestation increases oxidative stress to the fetus, as measured by fetal liver H2O2 and MDA.
Hostetler
C E
CE
Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USA.
Kincaid
R L
RL
D000818:Animals; D004032:Diet; D005260:Female; D005333:Fetus / Q000378:metabolism; D005865:Gestational Age; D005979:Glutathione Peroxidase / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008099:Liver / Q000196:embryology / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D008431:Maternal-Fetal Exchange; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D012643:Selenium / Q000008:administration & dosage / Q000172:deficiency*; D013552:Swine
D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D012643:Selenium