Model-based analysis and optimization of an ISPR approach using reactive extraction for pilot-scale L-phenylalanine production.
Based on experimental data from fermentation runs, as well as from L-phenylalanine (l-Phe) separation studies, a simple model is presented that describes the total ISPR approach for on-line L-Phe separation. While fermentation process modeling via a macrokinetic model revealed an L-Phe inhibition constant of 20 +/- 1.35 g/L using recombinant E. coli cells, the reactive-extraction process modeling identified the L-Phe cation diffusion in the aqueous donor film and the transport of the lowly soluble carrier/L-Phe complex in the aqueous acceptor film as the most dominant transfer steps. The corresponding mass transfer coefficients were estimated as k(PheD) = 128 x 10(-7) cm/s (extraction) and k(CPheA) = 178 x 10(-5) cm/s (back-extraction). Simulation studies were performed for the total ISPR approach, which gave hints for strategies of further process optimization.
Takors
R
R
Institute of Biotechnology, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany. r.takors@fz-juelich.de
D000465:Algorithms; D019149:Bioreactors / Q000382:microbiology*; D003198:Computer Simulation; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D005590:Fractional Precipitation; D007218:Industrial Microbiology / Q000379:methods; D007700:Kinetics; D008954:Models, Biological; D008956:Models, Chemical; D010649:Phenylalanine / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D010865:Pilot Projects; D011786:Quality Control; D014462:Ultrafiltration / Q000379:methods
D010649:Phenylalanine