Structural analysis of neprilysin with various specific and potent inhibitors.
Neutral endopeptidase (NEP) is the major enzyme involved in the metabolic inactivation of a number of bioactive peptides including the enkephalins, substance P, endothelin, bradykinin and atrial natriuretic factor. Owing to the physiological importance of NEP in the modulation of nociceptive and pressor responses, there is considerable interest in inhibitors of this enzyme as novel analgesics and antihypertensive agents. Here, the crystal structures of the soluble extracellular domain of human NEP (residues 52-749) complexed with various potent and competitive inhibitors are described. The structures unambiguously reveal the binding mode of the different zinc-chelating groups and the subsite specificity of the enzyme.
Oefner
Christian
C
Morphochem AG, CH-4058 Basel, Switzerland.
Roques
Bernard P
BP
Fournie-Zaluski
Marie-Claude
MC
Dale
Glenn E
GE
D001667:Binding, Competitive; D018360:Crystallography, X-Ray; D004583:Electrons; D004745:Enkephalins / Q000737:chemistry; D006801:Humans; D008956:Models, Chemical; D008958:Models, Molecular; D015260:Neprilysin / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D015032:Zinc / Q000737:chemistry
D004745:Enkephalins; D010455:Peptides; D015260:Neprilysin; D015032:Zinc