Purification, crystallization and preliminary X-ray studies of sylvaticin, an elicitin-like protein from Pythium sylvaticum.
Sylvaticin belongs to the elicitin family. These 10 kDa oomycetous proteins induce a hypersensitive response in plants, including necrosis and cell death, but subsequently leading to a non-specific systemic acquired resistance (SAR) against other pathogens. Sylvaticin has been crystallized using PEG 2000 MME as a precipitant agent in the presence of nickel chloride. The crystals belong to space group C2, with unit-cell parameters a = 99.29, b = 25.67, c = 67.45 A, beta = 99.66 degrees. Diffraction data were recorded to 2.1 A resolution at a synchrotron-radiation source.
Lascombe
Marie-Bernard
MB
CNRS UMR 8015, Faculté de Pharmacie, 4 Avenue de l'Observatoire, 75270 Paris CEDEX 06, France. marie-bernard.lascombe@univ-paris5.fr
Ponchet
Michel
M
Cardin
Loic
L
Milat
Marie-Louise
ML
Blein
Jean-Pierre
JP
Prangé
Thierry
T
D020418:Algal Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D005663:Furans / Q000737:chemistry* / Q000302:isolation & purification; D009532:Nickel / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011487:Protein Conformation; D011506:Proteins; D011775:Pythium / Q000378:metabolism*; D012997:Solvents / Q000737:chemistry; D017356:Synchrotrons
D020418:Algal Proteins; D005663:Furans; D011506:Proteins; D012997:Solvents; C085101:elicitin, Phytophthora; C063434:sylvaticin; D011092:Polyethylene Glycols; C022838:nickel chloride; D009532:Nickel