Mitochondrial diabetes: molecular mechanisms and clinical presentation.
Mutations in mitochondrial DNA (mtDNA) associate with various disease states. A few mtDNA mutations strongly associate with diabetes, with the most common mutation being the A3243G mutation in the mitochondrial DNA-encoded tRNA(Leu,UUR) gene. This article describes clinical characteristics of mitochondrial diabetes and its molecular diagnosis. Furthermore, it outlines recent developments in the pathophysiological and molecular mechanisms leading to a diabetic state. A gradual development of pancreatic beta-cell dysfunction upon aging, rather than insulin resistance, is the main mechanism in developing glucose intolerance. Carriers of the A3243G mutation show during a hyperglycemic clamp at 10 mmol/l glucose a marked reduction in first- and second-phase insulin secretion compared with noncarriers. The molecular mechanism by which the A3243G mutation affects insulin secretion may involve an attenuation of cytosolic ADP/ATP levels leading to a resetting of the glucose sensor in the pancreatic beta-cell, such as in maturity-onset diabetes of the young (MODY)-2 patients with mutations in glucokinase. Unlike in MODY2, which is a nonprogressive form of diabetes, mitochondrial diabetes does show a pronounced age-dependent deterioration of pancreatic function indicating involvement of additional processes. Furthermore, one would expect that all mtDNA mutations that affect ATP synthesis lead to diabetes. This is in contrast to clinical observations. The origin of the age-dependent deterioration of pancreatic function in carriers of the A3243G mutation and the contribution of ATP and other mitochondrion-derived factors such as reactive oxygen species to the development of diabetes is discussed.
Maassen
J Antonie
JA
Department of Molecular Cell Biology, Leiden University Medical Centre, Leiden, the Netherlands. j.a.maassen@lumc.nl
'T Hart
Leen M
LM
Van Essen
Einar
E
Heine
Rob J
RJ
Nijpels
Giel
G
Jahangir Tafrechi
Roshan S
RS
Raap
Anton K
AK
Janssen
George M C
GM
Lemkes
Herman H P J
HH
D000818:Animals; D001483:Base Sequence; D003920:Diabetes Mellitus / Q000453:epidemiology / Q000235:genetics*; D018149:Glucose Intolerance / Q000235:genetics; D006801:Humans; D015994:Incidence; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012356:RNA, Transfer, Leu / Q000235:genetics*
D007328:Insulin; D012356:RNA, Transfer, Leu