Stability and solubility of integral membrane proteins from photosynthetic bacteria solubilized in different detergents.
As a first step toward the establishment of practical guidelines for the search for crystallization conditions, stability and solubility were examined for integral membrane proteins from photosynthetic bacteria in the presence of different detergents. The results obtained from their stability provided practical information on the proper choice of detergent type in the preparation process and the subsequent crystallization experiment. In addition, the determination of a solubility diagram provided a practical method for quantifying the correct choice of detergent concentration and for setting up the suitable precipitant concentration in the crystallization experiment.
Odahara
Takayuki
T
National Institute of Advanced Industrial Science and Technology, Tsukuba Central-6, 1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. odahara-takayuki@aist.go.jp
D011232:Chemical Precipitation; D003460:Crystallization; D003902:Detergents / Q000737:chemistry*; D008320:Maltose / Q000031:analogs & derivatives* / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry*; D016354:Rhodobacter capsulatus / Q000737:chemistry*; D012242:Rhodobacter sphaeroides / Q000737:chemistry*; D012995:Solubility; D014961:X-Ray Diffraction
D003902:Detergents; D008565:Membrane Proteins; C411362:dodecyl maltopyranoside; D008320:Maltose