title: Evidence of association of the CYP2E1 genetic polymorphism with micronuclei frequency in human peripheral blood.
abstract: Micronuclei (MN) are used as one of the cytogenetic biomarkers, and intra- and inter-individual variations in this frequency have been reported in human blood lymphocytes. Polymorphisms in a few metabolic enzyme genes seem to account for a proportion of this variability, but the impacts of specific genetic variants on the MN frequency have not yet been clarified. Here, we investigated the relationship between the MN frequency and several gene polymorphisms in 90 healthy Japanese men. The subjects with the CYP2E1(*)3 variant allele had a statistically lower mean MN frequency than subjects with the CYP2E1(*)1/(*)1 wild type. Furthermore, the adjusted odds ratio (OR) of the CYP2E1(*)3 variant with higher MN frequency levels was also significantly lower and calculated to be 0.25 (95% CI 0.07-0.83), when the OR for the subjects with the CYP2E1(*)1/(*)1 wild type was defined as 1.00. These data suggest that the CYP2E1(*)3 polymorphism may have the potential to influence the baseline frequency of MN.
authors.affiliation: Department of Public Health and Preventive Medicine, Mie University School of Medicine, Edobashi 2-174, Tsu 514-8507, Japan. jinishi@doc.medic.mie-u.ac.jp
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ishikawa
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Ying
authors.initials: Y
authors.lastname: Tian
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Kawano
authors.forename: Toru
authors.initials: T
authors.lastname: Yamauchi
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Yokoyama
mesh_terms: D000328:Adult; D001769:Blood; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D048629:Micronuclei, Chromosome-Defective; D008875:Middle Aged; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D012016:Reference Values
chemical_list: D019392:Cytochrome P-450 CYP2E1; D019363:Cytochrome P-450 CYP1A1; D005982:Glutathione Transferase