Real-time measurement of in vitro flow by Fourier-domain color Doppler optical coherence tomography.
The possibility of measuring a full Doppler flow depth profile in parallel by use of frequency-domain optical coherence tomography is demonstrated. The method is based on a local phase analysis of the backscattered signal and allows for imaging of bidirectional Doppler flow. The Doppler frequency limit is 5 kHz for the presented measurements and is set by half of the frame rate of the CCD detector array. We measured the flow of 0.3-microm microspheres suspended in distilled water at controlled flow rates and in vitro human blood flow through a 200-microm capillary with a real-time color-encoded Doppler tomogram rate of 2-3/s.
Leitgeb
Rainer A
RA
Department of Medical Physics, University of Vienna, Waehringer Strasse 13, Vienna A-1090, Austria. rainer.leitgeb@univie.ac.at
Schmetterer
Leopold
L
Hitzenberger
Christoph K
CK
Fercher
Adolf F
AF
Berisha
Fatma
F
Wojtkowski
Maciej
M
Bajraszewski
Tomasz
T
D001773:Blood Cells / Q000502:physiology / Q000528:radiation effects; D001808:Blood Vessels / Q000502:physiology*; D003199:Computer Systems; D005583:Fourier Analysis; D006801:Humans; D066298:In Vitro Techniques; D008863:Microspheres; D008955:Models, Cardiovascular; D012039:Regional Blood Flow; D012212:Rheology; D012542:Scattering, Radiation; D041623:Tomography, Optical Coherence; D018615:Ultrasonography, Doppler, Color