title: Collagenase-1 (MMP-1) activity in equine synovial fluid: influence of age, joint pathology, exercise and repeated arthrocentesis.
abstract: BACKGROUND
  Matrix metalloproteinases (MMPs) are considered candidate biomarkers for both physiological and pathological tissue remodelling because of their key role in articular cartilage homeostasis. As disruption of the collagenous architecture is thought to be pivotal in chronic degenerative diseases such as osteoarthritis (OA), the collagenases form an interesting subset of the MMPs. The significance of any biomarker in synovial fluid (SF) can be assessed properly only when fluctuations in patterns induced by physiological processes such as development and growth, and by external influences and interventions such as exercise and repeated arthrocentesis, are known and taken into account.
  
  
  OBJECTIVE
  To investigate the activity of MMP-1 in equine SF at different stages of development and in joints affected by OA, and the influence of exercise and repeated arthrocentesis thereon.
  
  
  METHODS
  MMP-1 activity was determined in SF of normal joints of fetal, juvenile and mature horses, and in SF of horses suffering from OA, using an internally quenched fluorogenic peptide substrate. MMP-1 activity was also measured in SF from horses subjected to an exercise regimen and those subjected to repeated arthrocentesis.
  
  
  RESULTS
  An age-related decline in the SF levels of active MMP-1 was observed. MMP-1 activity was 15-fold higher in fetal than in juvenile animals, which showed significantly higher MMP-1 activity levels than mature horses. In SF of OA joints, MMP-1 activity was increased. Exercise did not affect MMP-1 activity in SF, but repeated arthrocentesis (within 60 h) increased MMP-1 activity significantly.
  
  
  CONCLUSIONS
  The high MMP-1 activity in SF of young individuals parallels the high metabolic activity occurring during rapid growth and differentiation at early age. The elevated MMP-1 activity in SF of OA joints probably reflects pathological matrix degradation, confirming the potential of MMP-1 to serve as a biochemical marker for early joint disease. Moderate exercise is not likely to influence the outcome of MMP-1 activity measurements in equine SF, but arthrocentesis should be taken into account as a possible confounding factor.
  
  
  CONCLUSIONS
  Given the crucial role of the collagen matrix for tissue integrity, MMP-1 activity may be a useful tool in diagnostic, therapeutic or prognostic studies in horses suspected of OA. However, care should be taken to exclude fluctuations in MMP-1 activity induced by physiological processes such as development and growth, and by interventions such as repeated arthrocentesis.
authors.affiliation: Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht, The Netherlands.
authors.forename: P A J
authors.initials: PA
authors.lastname: Brama
authors.forename: R
authors.initials: R
authors.lastname: van den Boom
authors.forename: J
authors.initials: J
authors.lastname: DeGroott
authors.forename: G H
authors.initials: GH
authors.lastname: Kiers
authors.forename: P R
authors.initials: PR
authors.lastname: van Weeren
mesh_terms: D000367:Age Factors; D000375:Aging / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D005333:Fetus / Q000201:enzymology; D006734:Horse Diseases / Q000175:diagnosis / Q000201:enzymology*; D006736:Horses; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism*; D010003:Osteoarthritis / Q000201:enzymology / Q000662:veterinary*; D019152:Paracentesis / Q000009:adverse effects / Q000662:veterinary; D010805:Physical Conditioning, Animal / Q000502:physiology; D013582:Synovial Fluid / Q000201:enzymology*
chemical_list: D015415:Biomarkers; D020781:Matrix Metalloproteinase 1