Comparison of different adjuvants of protein and DNA vaccination for the prophylaxis of IgE antibody formation.
A high-molecular-weight mite allergen Der f11 that was hardly purified for immunotherapy was used to develop the DNA vaccine pDf11. We have shown that vaccination of mice with pDf11 induces Th1 responses characterized by suppression of IgE responses. In the present study, effects of different adjuvants on pDf11 were first studied. Mice receiving pDf11 +/- CpG, bestatin, and bupivacaine had better suppression of IgE responses than those receiving pDf11 +/- lipofectin or alum. Bestatin could greatly boost IgG2a responses. Immunomodulating effects of different adjuvants between protein and DNA vaccines were further elucidated. CpG was the best for both protein and DNA vaccines to profoundly suppress IgE responses, but alum, bestatin and lipofectin were useless for rDf11 to induce IgE inhibition. Neither did the combination of rDf11 and pDf11 have further IgE suppression. In conclusion, CpG is the unique adjuvant for the protein vaccine rDf11 to inhibit IgE responses. In contrast, the DNA vaccine pDf11 +/- CpG, bestatin, or bupivacaine induces profound suppression of IgE responses.
Peng
Ho-Jen
HJ
Department of Medical Research and Education, Veterans General Hospital-Taipei, Taipei 11217, Taiwan. hjpeng@vghtpe.gov.tw
Tsai
Lai-Chen
LC
Su
Song-Nan
SN
Chang
Zo-Nan
ZN
Shen
Horng-Der
HD
Chao
Pei-Ling
PL
Kuo
Shu-Wen
SW
Tsao
I-Yu
IY
Hung
Mei-Whey
MW
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000485:Allergens / Q000276:immunology; D000534:Alum Compounds / Q000494:pharmacology; D000818:Animals; D002626:Chemistry, Pharmaceutical; D018899:CpG Islands / Q000187:drug effects; D005260:Female; D007073:Immunoglobulin E / Q000032:analysis / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D007930:Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008925:Mites / Q000276:immunology; D010714:Phosphatidylethanolamines / Q000494:pharmacology; D010957:Plasmids / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D019444:Vaccines, DNA / Q000276:immunology*; D022223:Vaccines, Subunit / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000485:Allergens; D000534:Alum Compounds; D007074:Immunoglobulin G; D010714:Phosphatidylethanolamines; D019444:Vaccines, DNA; D022223:Vaccines, Subunit; C041524:aluminum sulfate; D007073:Immunoglobulin E; C020888:1,2-dielaidoylphosphatidylethanolamine; D007930:Leucine; C012211:ubenimex