title: Molecular cloning and characterization of a novel human putative transmembrane protein homologous to mouse sideroflexin associated with sideroblastic anemia.
abstract: Sideroflexin1 (Sfxn1), the prototype of a novel family of evolutionarily conserved proteins present in eukaryotes, has been found mutated in mice with siderocytic anemia. It is speculated that this protein facilitates the transport of a component required for iron utilization into mitochondrial. During the large-scale sequencing analysis of a human fetal brain cDNA library, we isolated a cDNA encoding a novel sideroflexin protein (SFXN4), which showed 59% identity and 71% similarity to mouse sideroflexin4. According to the search of the human genome database, SFXN4 gene is mapped to chromosome 10q25-26 and spans more than 24.7kb of the genomic DNA. It is 1428 base pair in length and the putative protein contains 305 amino acids with a conserved predicted five-transmembrane-domains structure. RT-PCR result shows that the SFXN4 gene is expressed in many tissues.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
authors.forename: Huarui
authors.initials: H
authors.lastname: Zheng
authors.forename: Chaoneng
authors.initials: C
authors.lastname: Ji
authors.forename: Xianqiong
authors.initials: X
authors.lastname: Zou
authors.forename: Maoqing
authors.initials: M
authors.lastname: Wu
authors.forename: Zhe
authors.initials: Z
authors.lastname: Jin
authors.forename: Gang
authors.initials: G
authors.lastname: Yin
authors.forename: Jixi
authors.initials: J
authors.lastname: Li
authors.forename: Congjing
authors.initials: C
authors.lastname: Feng
authors.forename: Haipeng
authors.initials: H
authors.lastname: Cheng
authors.forename: Shaohua
authors.initials: S
authors.lastname: Gu
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
authors.forename: Yumin
authors.initials: Y
authors.lastname: Mao
mesh_terms: D000595:Amino Acid Sequence; D000756:Anemia, Sideroblastic / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D027682:Cation Transport Proteins / Q000737:chemistry / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D015723:Gene Library; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D027682:Cation Transport Proteins; D018076:DNA, Complementary; D008565:Membrane Proteins; C488377:SFXN4 protein, human; C424902:Sfxn1 protein, mouse