[RT-PCR method for detecting the expression of transforming growth factor-beta1 gene in lung tissues of silica-treated mice].
OBJECTIVE
To investigate the gene expression of transforming growth factor-beta(1) (TGF-beta(1)) in lung tissues of silica-treated mice.


METHODS
The experimental mice were divided into control and silica group. 0.2 g/kg body weight of silica was injected intratracheally in silica group. Samples of lung tissue were collected 1, 3, 5, 7, 14 and 28 d after injection. RT-PCR method was used to analyze the gene expression of TGF-beta(1) in lung tissue of silica-treated mice.


RESULTS
The expression of TGF-beta(1) gene in lung tissue elevated from the 3rd day (1.20 +/- 0.15) and the peak value was on the 7th day (1.74 +/- 0.19). Then the expression decreased from the 14th to 28th day. But there was still higher than control until the 28th day.


CONCLUSIONS
TGF-beta(1) may play an important role in silica-induced pulmonary fibrosis.
Ji
Wen-jie
WJ
Institute of Occupational Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Yang
Lei
L
Wang
Zheng-lun
ZL
Ding
Jia-shun
JS
Liu
Cang
C
He
Han-zhen
HZ
D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D011658:Pulmonary Fibrosis / Q000209:etiology / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012822:Silicon Dioxide / Q000494:pharmacology* / Q000633:toxicity; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000235:genetics*
D016212:Transforming Growth Factor beta; D012822:Silicon Dioxide