Receptor-like protein tyrosine phosphatase zeta/RPTP beta is expressed on tangentially aligned neurons in early mouse neocortex.
Protein tyrosine phosphatase zeta (PTPzeta)/RPTPbeta is a chondroitin sulfate proteoglycan predominantly expressed in the brain. In this study, we examined immunohistochemical localisation of PTPzeta in the mouse telencephalon from embryonic day 9.5 (E9.5) to E15.5. During E10.5-E12.5, immunoreactivities for PTPzeta are specifically observed on the tangentially aligned neurons at the preplate (PP) of the neocortex, as well as on the neurons at the mantle layer (ML) of the ganglionic eminences (GEs). Likewise, neurons immunoreactive for CR50, a marker for Cajal-Retzius neurons, are aligned from the ML of the ganglionic eminences to the PP of the neocortex and co-express PTPzeta. During E13.5-E15.5, PTPzeta-positive neurons are present at the subplate (SP) as well as at the marginal zone (MZ) of the neocortex. These results indicate that PTPzeta is a useful marker for early-generated neocortical neurons in mice: Cajal-Retzius neurons as well as the subplate neurons.
Ohyama
Kyoji
K
Department of Anatomy, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan. K.Ohyama@Sheffield.ac.uk
Ikeda
Emi
E
Kawamura
Koki
K
Maeda
Nobuaki
N
Noda
Masaharu
M
D000375:Aging; D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D064026:Calbindins; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism; D004622:Embryo, Mammalian; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D008818:Mice, Neurologic Mutants; D019579:Neocortex / Q000166:cytology* / Q000196:embryology; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D054630:Receptor-Like Protein Tyrosine Phosphatases, Class 4; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5; D000091042:Reelin Protein; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D012697:Serine Endopeptidases; D014404:Tubulin / Q000378:metabolism
D064026:Calbindins; D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D018809:Proliferating Cell Nuclear Antigen; D000091042:Reelin Protein; D064030:S100 Calcium Binding Protein G; D014404:Tubulin; C462941:beta3 tubulin, mouse; D017027:Protein Tyrosine Phosphatases; C098067:Ptpre protein, mouse; C516896:Ptprz1 protein, mouse; D054630:Receptor-Like Protein Tyrosine Phosphatases, Class 4; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5; D012697:Serine Endopeptidases; D001973:Bromodeoxyuridine