title: Auditory brainstem evoked response in juvenile rats fed rat milk formulas with high docosahexaenoic acid.
abstract: Previous studies found that juvenile offspring of rats fed high docosahexaenoic acid (DHA; 22:6n-3) diets through gestation and lactation had longer auditory brainstem-evoked response (ABR) accompanied by higher 22:6n-3 and lower arachidonic acid (ARA; 20:4n-6) in brain. In the present study, ABR was assessed in juvenile rats fed high-DHA diets only postnatally.
  
  
  METHODS
  Rat pups were fed rat milk formulas with varying amounts of DHA and ARA to 19 days of age followed by diets with the corresponding fatty acids. The high-DHA group was fed 2.3% of fatty acids as DHA, the DHA + ARA group was fed DHA and ARA at 0.6 and 0.4% of fatty acids, levels similar to those in some infant formulas, and the unsupplemented group was fed no DHA or ARA. ABR and fatty acid and monoamine levels in brain were measured on postnatal days 26-28. Statistical analyses were measured by ANOVA.
  
  
  RESULTS
  ARA and DHA levels in brain increased with supplementation. ABR was shorter in the high-DHA group than the DHA + ARA group and not different from the unsupplemented or dam-reared suckling group. Norepinephrine levels in the inferior colliculus were lower in the high-DHA group than the DHA + ARA group and higher in all formula groups compared to the dam-reared group.
  
  
  CONCLUSIONS
  In contrast to the longer ABR in juvenile offspring of rats fed high-DHA through gestation and lactation, ABR was shorter in juvenile rats fed high-DHA diets only after birth than rats fed ARA + DHA. Further studies are needed to understand the relationship between dietary DHA, norepinephrine, and auditory system development over a range of DHA intakes and discrete periods of development.
authors.affiliation: 625 Cleveland Avenue, Ross Products Division, Abbott Laboratories, Columbus, OH 43215, USA. nancy.auestad@abbott.com
authors.forename: Nancy
authors.initials: N
authors.lastname: Auestad
authors.forename: Janet
authors.initials: J
authors.lastname: Stockard-Sullivan
authors.forename: Sheila M
authors.initials: SM
authors.lastname: Innis
authors.forename: Rose
authors.initials: R
authors.lastname: Korsak
authors.forename: John
authors.initials: J
authors.lastname: Edmond
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000008:administration & dosage / Q000032:analysis; D015306:Biogenic Monoamines / Q000032:analysis; D001921:Brain / Q000254:growth & development; D001923:Brain Chemistry; D004281:Docosahexaenoic Acids / Q000008:administration & dosage* / Q000032:analysis*; D016057:Evoked Potentials, Auditory, Brain Stem / Q000502:physiology*; D005227:Fatty Acids / Q000032:analysis; D005526:Food, Formulated / Q000032:analysis*; D007245:Inferior Colliculi / Q000737:chemistry; D008892:Milk; D009929:Organ Size; D010743:Phospholipids / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015430:Weight Gain
chemical_list: D015306:Biogenic Monoamines; D005227:Fatty Acids; D010743:Phospholipids; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid