Temperature-dependent Raman spectra of collagen and DNA.
Raman spectra of collagen and DNA were discussed at different temperatures. The temperature-dependence of Raman intensity was obtained in the region from -150 to 200 degrees C. Four denaturation points at 0, 40, 68 and 90 degrees C of collagen and two peaks at 38 and 82 degrees C for DNA were obtained. The wavenumbers of many vibrational modes were found to increase for lower temperature, but the peak at 1302 cm(-1) of collagen and the peak at 1101 cm(-1) of DNA showed the opposite trend. In all of the vibrational modes of DNA, the bases showed the most sensitive to different temperatures and there is a pronounced shift of bands at 70 degrees C, the starting point of denaturation.
Dong
Ruixin
R
School of Physical Electronics, University of Electronic Science and Technology of China, Chengdu, Sichuan 610054, China. ruixindong@163.com
Yan
Xunling
X
Pang
Xiaofeng
X
Liu
Shenggang
S
D000818:Animals; D002417:Cattle; D003094:Collagen / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D013059:Spectrum Analysis, Raman; D013696:Temperature; D013950:Thymus Gland / Q000737:chemistry
D003094:Collagen; D004247:DNA