title: Serotonin activity and liver dysfunction following hepatic ischemia and reperfusion.
abstract: Serotonin (5-hydroxytriptamine; 5-HT), which is stored in platelets, is known to induce vasoconstriction and promote platelet aggregation. More recent studies suggest that serotonin also plays a role in organ injury after ischemia and reperfusion. The purpose of this study was to characterize the role of 5-HT and platelet function in the pathogenesis of hepatic ischemia-reperfusion injury. Under the portocaval shunt, 60 or 90 min of complete warm ischemia of canine liver was induced by Pringle's maneuver, followed by reperfusion for 120 min. Time-matched, sham-operated animals served as controls. Hepatic tissue blood flow and various parameters of hepatic vein blood (ALT, LDH, platelet count and platelet aggregation) were measured before and after reperfusion. 5-HT levels in portal vein and hepatic vein were also assayed. Hepatic ischemia and reperfusion resulted in liver hypoperfusion, hepatocellular dysfunction, increased platelet aggregation, increased 5-HT levels, and hepatic microcirculation injury. These results suggest that the endogenous 5-HT released from platelet may contribute to liver tissue hypoperfusion following hepatic ischemia-reperfusion.
authors.affiliation: Second Department of Surgery, Faculty of Medicine, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-8520, Japan.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Murata
authors.forename: Nobuo
authors.initials: N
authors.lastname: Hamada
authors.forename: Noboru
authors.initials: N
authors.lastname: Nakamura
authors.forename: Akira
authors.initials: A
authors.lastname: Kobayashi
authors.forename: Mikio
authors.initials: M
authors.lastname: Fukueda
authors.forename: Akira
authors.initials: A
authors.lastname: Taira
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Sakata
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008102:Liver Circulation; D008107:Liver Diseases / Q000378:metabolism* / Q000473:pathology; D008297:Male; D010974:Platelet Aggregation; D010976:Platelet Count; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D012701:Serotonin / Q000097:blood*
chemical_list: D012701:Serotonin; D007770:L-Lactate Dehydrogenase; D000410:Alanine Transaminase