title: Acute and persistent experimental nodavirus infection in spotted wolffish Anarhichas minor.
abstract: Spotted wolffish Anarhichas minor (approx. 0.7 g) were found to be susceptible to infection with a nodavirus isolated from Atlantic halibut (AHNV) by bath-challenge. During the acute stage of infection, 4 to 8 wk post-challenge, viral encephalopathy and retinopathy (VER) were diagnosed by histopathology, immunohistochemistry (IHC) and reverse transcriptase-polymerase chain reaction (RT-PCR). Accumulated mortality was 52% in the challenged group. The surviving fish were sampled 16 wk post-challenge, by which time they had grown to approximately 17 g. No clinical signs of VER were observed in these fish. RT-PCR examination revealed the presence of nodavirus in several organs of the survivors, but no immunopositive cells were detected by IHC. Nodavirus was reisolated from fish at the last sampling in SSN-1 cells, showing that nodavirus retains virulence in persistently infected wolffish for at least 16 wk post-bath-challenge.
authors.affiliation: National Veterinary Institute, PO Box 8156, 0033 Oslo, Norway. renate.johansen@vetinst.no
authors.forename: R
authors.initials: R
authors.lastname: Johansen
authors.forename: M
authors.initials: M
authors.lastname: Amundsen
authors.forename: B H
authors.initials: BH
authors.lastname: Dannevig
authors.forename: A I
authors.initials: AI
authors.lastname: Sommer
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D017931:DNA Primers; D005393:Fish Diseases / Q000473:pathology / Q000821:virology*; D006652:Histological Techniques; D007150:Immunohistochemistry; D030381:Nodaviridae / Q000302:isolation & purification* / Q000472:pathogenicity; D009664:Norway; D010473:Perciformes / Q000821:virology*; D012327:RNA Virus Infections / Q000635:transmission / Q000662:veterinary*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers