Evolution of the gene encoding mitochondrial intermediate peptidase and its cosegregation with the A mating-type locus of mushroom fungi.
The high level of DNA polymorphism at the mating-type loci of mushroom fungi has made the cloning of mating-type genes difficult. As an alternative to strategies that employ sequence conservation, an approach utilizing conserved gene order could facilitate the cloning of A mating-type genes from mushroom fungi. It has been shown that a gene encoding a mitochondrial intermediate peptidase (MIP) is very close ( < 1 kbp) to the A mating-type locus of two model mushroom species. In this study, the cosegregation of MIP and the A mating-type locus was studied by genotyping progeny of seven additional mushroom species using PCR and genetic crosses. No evidence of any recombination between MIP and the A mating-type locus was detected among all seven species. Phylogenetic analysis of MIP sequences from diverse mushroom species agrees with the current organismal phylogeny, suggesting the sequences are generally orthologous.
James
Timothy Y
TY
Department of Biology, Box 90338, Duke University, Durham, NC 27708, USA. tyj2@duke.edu
Kües
Ursula
U
Rehner
Stephen A
SA
Vilgalys
Rytas
R
D000363:Agaricales / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D014644:Genetic Variation; D000072235:Mating Factor; D008666:Metalloendopeptidases / Q000235:genetics*; D010455:Peptides / Q000235:genetics*; D010802:Phylogeny
D017931:DNA Primers; D004271:DNA, Fungal; D010455:Peptides; D000072235:Mating Factor; D008666:Metalloendopeptidases; C068038:mitochondrial intermediate peptidase