p-Nonylphenol, 4-tert-octylphenol and bisphenol A increase the expression of progesterone receptor mRNA in the frontal cortex of adult ovariectomized rats.
Alkylphenols, such as p-nonylphenol (NP) and 4-tert-octylphenol (OP) and bisphenol A (BPA) are thought to mimic oestrogens in their action, and are called endocrine disrupters. We examined whether these endocrine disrupters affected progesterone receptor (PR) mRNA expression in the adult female rat neocortex. In one experiment, at 12.00 h, ovariectomized rats were given a subcutaneous injection of 10 mg of NP, 10 mg of OP or 10 mg of BPA, or sesame oil alone as control. Twenty-four hours after injection, the left side of the frontal cortex, parietal cortex and temporal cortex was collected. In a second experiment to study the time-course of the effects of BPA on PR mRNA, the ovariectomized rats were given a subcutaneous injection of 10 mg of BPA and killed 0, 6, 12 and 24 h after injection. In addition to the frontal cortex and temporal cortex, the occipital cortex was also collected. Northern blotting revealed that, in the first experiment, injection of NP, OP or BPA significantly increased PR mRNA expression in the frontal cortex but not in the parietal cortex. In the temporal cortex, BPA significantly decreased PR mRNA, but NP and OP produced no significant changes. The second experiment revealed that, in the frontal cortex, BPA induced a significant increase in PR mRNA expression at 6 h after injection, which lasted until 24 h after injection. In the temporal cortex, PR mRNA expression was significantly decreased 6 h after injection of BPA and was still significantly low 24 h after injection. No significant change was observed in the occipital cortex. These results suggest that, even in adult rats, endocrine disrupters alter the neocortical function by affecting the PR system, although the physiological significance of PR in the affected area is unknown.
Funabashi
T
T
Neuroendocrinology, Yokohama City University Graduate School of Medicine, Fukuura, Yokohama, Japan. toshiya@med.yokohama-cu.ac.jp
Nakamura
T J
TJ
Kimura
F
F
D000818:Animals; D001559:Benzhydryl Compounds; D015152:Blotting, Northern; D002540:Cerebral Cortex / Q000378:metabolism*; D004785:Environmental Pollutants / Q000633:toxicity*; D004967:Estrogens / Q000819:agonists; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D005260:Female; D005625:Frontal Lobe / Q000378:metabolism; D010052:Ovariectomy; D010296:Parietal Lobe / Q000378:metabolism; D010636:Phenols / Q000633:toxicity*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D011980:Receptors, Progesterone / Q000235:genetics / Q000378:metabolism*; D013702:Temporal Lobe / Q000378:metabolism; D013997:Time Factors; D014018:Tissue Distribution; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004785:Environmental Pollutants; D004967:Estrogens; D004968:Estrogens, Non-Steroidal; D010636:Phenols; D012333:RNA, Messenger; D011980:Receptors, Progesterone; C025256:nonylphenol; C105260:4-tert-octylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds