title: In vivo formation of glutamyl-tRNA(Gln) in Escherichia coli by heterologous glutamyl-tRNA synthetases.
abstract: Two types of glutamyl-tRNA synthetase exist: the discriminating enzyme (D-GluRS) forms only Glu-tRNA(Glu), while the non-discriminating one (ND-GluRS) also synthesizes Glu-tRNA(Gln), a required intermediate in protein synthesis in many organisms (but not in Escherichia coli). Testing the capacity to complement a thermosensitive E. coli gltX mutant and to suppress an E. coli trpA49 missense mutant we examined the properties of heterologous gltX genes. We demonstrate that while Acidithiobacillus ferrooxidans GluRS1 and Bacillus subtilis Q373R GluRS form Glu-tRNA(Glu), A. ferrooxidans and Helicobacter pylori GluRS2 form Glu-tRNA(Gln) in E. coli in vivo.
authors.affiliation: Programa de Biologi;a Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile, Casilla 70086, Santiago 838-0453, Chile.
authors.forename: Harold
authors.initials: H
authors.lastname: Núñez
authors.forename: Claudia
authors.initials: C
authors.lastname: Lefimil
authors.forename: Bokkee
authors.initials: B
authors.lastname: Min
authors.forename: Dieter
authors.initials: D
authors.lastname: Söll
authors.forename: Omar
authors.initials: O
authors.lastname: Orellana
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005975:Glutamate-tRNA Ligase / Q000235:genetics / Q000378:metabolism*; D020125:Mutation, Missense; D016133:Polymerase Chain Reaction; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D017931:DNA Primers; D029968:Escherichia coli Proteins; D012346:RNA, Transfer, Amino Acyl; C483187:glutamyl-tRNA(Gln); D005975:Glutamate-tRNA Ligase