title: Effects of enterally administering granulocyte colony-stimulating factor to suckling mice.
abstract: Gastrointestinal (GI) tract development is influenced by multiple growth factors, some of which are delivered directly to the GI lumen, as they are swallowed constituents of amniotic fluid, colostrum, and milk. Granulocyte colony-stimulating factor (G-CSF), traditionally known as a granulocytopoietic growth factor, is an example of one such factor. However, it is not clear whether the large amounts of G-CSF that are normally swallowed by the fetus and neonate have systemic effects on circulating neutrophils or local effects in the developing intestine. To assess this, we administered either active or heat-denatured (control) recombinant human G-CSF to 5- to 7-d-old C57BL/6 x 129SvJ mice. Pups received either a low dose (3 ng) that was calculated to approximate the amount of G-CSF swallowed in utero from amniotic fluid or an isovolemic high dose 100 times larger (300 ng). Oral dosing was performed daily for either 3 or 7 d, after which pups were killed and measurements were made on the blood and the GI tract. Absolute blood neutrophil counts and immature to total neutrophil ratios did not differ from controls in any of the test groups. However, intestinal villus area, perimeter, length, crypt depth, and proliferating cell nuclear antigen index increased significantly among those that were treated with active G-CSF. Thus, in suckling mice, enterally administered G-CSF had no effect on the concentration of circulating neutrophils but had trophic effects on the intestine. We speculate that the G-CSF present in amniotic fluid, colostrum, and milk acts as a topical intestinal growth factor and has little or no granulocytopoietic action.
authors.affiliation: Department of Pediatrics, University of Florida, Divsion of Neonatology, Gainesville, FL 32610, USA.
authors.forename: Jason A
authors.initials: JA
authors.lastname: Gersting
authors.forename: Robert D
authors.initials: RD
authors.lastname: Christensen
authors.forename: Darlene A
authors.initials: DA
authors.lastname: Calhoun
mesh_terms: D000284:Administration, Oral; D000653:Amniotic Fluid / Q000378:metabolism; D000818:Animals; D000833:Animals, Suckling; D002455:Cell Division; D003126:Colostrum / Q000378:metabolism; D004750:Enteral Nutrition; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage* / Q000627:therapeutic use*; D006358:Hot Temperature; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D020033:Protein Isoforms; D011994:Recombinant Proteins; D013997:Time Factors
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D020033:Protein Isoforms; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor