Gene expression profiling of breast carcinomas using nylon DNA arrays.
Clinically very heterogeneous, breast cancer prognosis and treatment response are difficult to predict with the current prognostic histoclinical parameters. Mammary oncogenesis remains poorly understood. DNA array technology allows the simultaneous analysis of the mRNA expression levels of thousands of genes in biological samples. Applied to breast tumours, expression profiles will boost our knowledge of oncogenesis, will offer new potential therapeutic targets and new prognostic and predictive markers. Today, the most accessible approach for academic research teams is that of Nylon DNA arrays with radioactive detection, which in addition allows profiling of small clinical samples.
Bertucci
François
F
Departement d'oncologie moléculaire, TAGC, Institut Paoli-Calmettes (IPC), IFR57, 232, bd Ste-Marguerite, 13273 Marseille cedex 9, France. bertuccif@marseille.fnclcc.fr
Loriod
Béatrice
B
Nasser
Valéry
V
Granjeaud
Samuel
S
Tagett
Rebecca
R
Braud
Anne-Chantal
AC
Patrice
Viens
V
Houlgatte
Rémi
R
Daniel
Birnbaum
B
Nguyen
Catherine
C
D001943:Breast Neoplasms / Q000145:classification / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D009757:Nylons; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011379:Prognosis
D009757:Nylons