title: Overexpression of DRG2 suppresses the growth of Jurkat T cells but does not induce apoptosis.
abstract: Developmentally regulated GTP-binding protein (DRG) is a new subfamily within the superfamily of GTP-binding proteins. Its expression is regulated during embryonic development. To investigate the effect of the expression of DRG2 on cell growth, we constructed a human Jurkat-T-cell line that overexpresses DRG2. Overexpression of DRG2 suppressed the growth and the aggregation of Jurkat cells but did not induce apoptotic cell death. We used cDNA microarray analysis to examine the global changes in gene expression induced by an overexpression of DRG2. DNA array analyses identified genes that may suppress cell growth at a number of levels in multiple signaling cascades in Jurkat cells and also several prosurvival genes that may protect cells from apoptosis.
authors.affiliation: Department of Biological Sciences and Immunomodulation Research Center, University of Ulsan, Ulsan 680-749, Republic of Korea.
authors.forename: Myoung Seok
authors.initials: MS
authors.lastname: Ko
authors.forename: Unn Hwa
authors.initials: UH
authors.lastname: Lee
authors.forename: Sin Il
authors.initials: SI
authors.lastname: Kim
authors.forename: Hyo Jeong
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jeong Jae
authors.initials: JJ
authors.lastname: Park
authors.forename: Seung Ju
authors.initials: SJ
authors.lastname: Cha
authors.forename: Sung Bum
authors.initials: SB
authors.lastname: Kim
authors.forename: Hebok
authors.initials: H
authors.lastname: Song
authors.forename: Dae Kyun
authors.initials: DK
authors.lastname: Chung
authors.forename: In Seob
authors.initials: IS
authors.lastname: Han
authors.forename: KyuBum
authors.initials: K
authors.lastname: Kwack
authors.forename: Jeong Woo
authors.initials: JW
authors.lastname: Park
mesh_terms: D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology*; D002449:Cell Aggregation; D002452:Cell Count; D002455:Cell Division / Q000502:physiology; D005434:Flow Cytometry; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D019169:Jurkat Cells; D020411:Oligonucleotide Array Sequence Analysis; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism / Q000502:physiology
chemical_list: D017304:Annexin A5; C409877:DRG2 protein, human; D011994:Recombinant Proteins; D019204:GTP-Binding Proteins