Isolation and expression pattern of two putative acyl-ACP desaturase cDNAs from Bassia scoparia.
The seed lipids of some higher plants contain unusual fatty acids with potentially valuable non-food uses. Seeds of Bassia scoparia contain one such monounsaturated fatty acid, 16:1Delta5. This fatty acid can be used for the production of an insect oviposition pheromone, which is potentially valuable in the control of the mosquito Culex quinquefasciatus, a vector of West Nile virus. Previous work has established that a number of unusual monounsaturated fatty acids are produced by variant forms of the ubiquitous acyl-ACP desaturases. The isolation and initial characterization of two putative acyl-ACP desaturases from B. scoparia, one of which is seed-specific, suggests that such a variant enzyme occurs in this species.
Whitney
Heather M
HM
Long Ashton Research Station, Long Ashton, Bristol BS41 9AF, UK.
Sayanova
Olga
O
Pickett
John A
JA
Napier
Johnathan A
JA
D019730:Carbon-Sulfur Ligases / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D029595:Orchidaceae / Q000201:enzymology / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification; D012639:Seeds / Q000201:enzymology / Q000235:genetics; D014158:Transcription, Genetic
D018749:RNA, Plant; D019730:Carbon-Sulfur Ligases; C018749:long-chain-fatty-acid-(acyl-carrier-protein) ligase