Nucleotide sequences of two Korean isolates of Cucumber green mottle mosaic virus.
The nucleotide sequences of the genomic RNAs of Cucumber green mottle mosaic virus Korean watermelon isolate (CGMMV-KW) and Korean oriental melon isolate (CGMMV-KOM) were determined and compared to the sequences of other tobamoviruses including CGMMV strains W and SH. Each CGMMV isolate had a genome of 6,424 nucleotides. Each also had 60 and 176 nucleotides of 5' and 3' untranslated regions (UTRs), respectively, and four open reading frames (ORF1-4). ORFs 1 to 4 encode proteins of 129, 186, 29, and 17.4 kDa, respectively. The nucleotide and deduced amino acid sequences of CGMMV-KOM and CGMMV-KW were more than 98.3% identical. When compared to other CGMMV strains in a phylogenetic analysis they were found to form a distinct virus clade, and were more distantly related to other tobamoviruses (23.5-56.7% identity).
Kim
Sang-Min
SM
School of Agricultural Biotechnology, Center for Plant Molecular Genetics and Breeding Research, Seoul National University, Seoul 151-742, Korea.
Lee
Jung-Myung
JM
Yim
Kyu-Ock
KO
Oh
Man-Ho
MH
Park
Jin-Woo
JW
Kim
Kook-Hyung
KH
D028463:Cucumis melo / Q000235:genetics / Q000821:virology; D018553:Cucumis sativus / Q000821:virology*; D007723:Korea; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D017898:Tobamovirus / Q000235:genetics*