Microglia-derived glial cell line-derived neurotrophic factor could protect Sprague-Dawley rat astrocyte from in vitro ischemia-induced damage.
The interrelationship between microglia and astrocytes in cerebral ischemia was determined in vitro by adding in vitro ischemia-induced supernatant from microglia into astrocytes under the same conditions (glucose-, oxygen- and serum-free). The involvement of glial cell line-derived neurotrophic factor (GDNF) was further investigated by immunoblocking assay and Western blot analysis. Results showed that microglia-derived supernatant protected against in vitro ischemia-induced damage of astrocytes and this protection was pre-blocked by anti-GDNF but not normal rabbit serum. In addition, in vitro ischemia appeared to induce the expression of GDNF in microglia. These results indicate that microglia-derived protection on astrocytes during in vitro ischemia is GDNF-dependent.
Lee
Gilbert A
GA
Department of Life Sciences, National Chung Hsing University, Taichung 40227, Taiwan.
Lin
Chi-Hsin
CH
Jiang
Hang-Hsiang
HH
Chao
Hsin-Ju
HJ
Wu
Chieh-Liang
CL
Hsueh
Chi-Mei
CM
D000818:Animals; D001253:Astrocytes / Q000187:drug effects*; D015153:Blotting, Western; D002478:Cells, Cultured; D051100:Glial Cell Line-Derived Neurotrophic Factor; D066298:In Vitro Techniques; D007511:Ischemia / Q000188:drug therapy*; D017628:Microglia / Q000502:physiology*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D018696:Neuroprotective Agents