Model of unidirectional transluminal gene transfer.
Gene transfer assays in vitro are poor indicators of transduction efficacy observed in vivo. We designed and optimized an intermediate model for assessing and quantifying unidirectional transduction ex vivo. The model enables simultaneous transmucosal evaluation of up to 96 different variables under the same tissue conditions. We show that the model is versatile and suitable for use with different vectors (adenovirus and AAV), different reporter genes (beta-galactosidase and green fluorescent protein), and viscera with various tissue features such as peritoneum and urothelium. Ex vivo transduction assays may correlate better with in vivo gene transfer results. Because the experimental model described here can be performed in small samples, it may enable translational applications in tissues of human origin.
Arap
Marco A
MA
Department of Genitourinary Medical Oncology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030, USA.
Lahdenranta
Johanna
J
Hajitou
Amin
A
Marini
Frank C
FC
Wood
Christopher G
CG
Wright
Kenneth C
KC
Fueyo
Juan
J
Arap
Wadih
W
Pasqualini
Renata
R
D000818:Animals; D015870:Gene Expression; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D010537:Peritoneum / Q000378:metabolism; D013552:Swine; D014161:Transduction, Genetic / Q000379:methods* / Q000592:standards; D019076:Transgenes / Q000235:genetics; D014513:Ureter / Q000378:metabolism
D006025:Glycosaminoglycans