[Studies on fluoride concentration and cholinesterase activity in rat hippocampus].
OBJECTIVE
To study the accumulation of fluoride in rat hippocampus and its effect on cholinesterase activity.


METHODS
Rats were subchronically exposed to NaF, and fluoride concentration and cholinesterase activity in rat hippocampus were determined.


RESULTS
Fluoride concentration in rat hippocampus was significantly correlated with the dosage of fluoride, and there were significant differences among high dosage group [(13.03 +/- 1.79) micro g/g], low dosage group [(9.83 +/- 0.92) micro g/g] and control [(8.27 +/- 1.11) micro g/g], P < 0.01. Acetylcholinesterase activities among three groups [(0.111 +/- 0.031) micro mol/mg, (0.143 +/- 0.025) micro mol/mg, (0.183 +/- 0.027) micro mol/mg] were also significantly different (P < 0.01), which was negatively correlated with fluoride concentration in rat hippocampus (r = -0.700, P < 0.01). The activity of butylcholinesterase in high dosage group [(0.041 +/- 0.010) micro mol/mg] was different from that of control [(0.067 +/- 0.025) micro mol/mg, P < 0.05], but the activity was not significantly related with fluoride concentration in rat hippocampus (r = -0.317, P = 0.094).


CONCLUSIONS
Fluoride may go through the blood-brain barrier and accumulate in rat hippocampus, and inhibit the activity of cholinesterase.
Zhai
Jin-xia
JX
School of Public Health, Anhui Medical University, Hefei 230032, China.
Guo
Zhi-yong
ZY
Hu
Chuan-lai
CL
Wang
Qi-nan
QN
Zhu
Qi-xing
QX
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001812:Blood-Brain Barrier; D002091:Butyrylcholinesterase / Q000378:metabolism*; D005458:Fluoride Poisoning / Q000378:metabolism*; D005459:Fluorides / Q000493:pharmacokinetics*; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D009929:Organ Size; D051381:Rats; D017207:Rats, Sprague-Dawley
D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D005459:Fluorides