Depletion of host reactive T cells by photodynamic cell purging and prevention of graft versus host disease.
Graft versus Host Disease (GVHD) is the principal cause of morbidity and mortality in patients undergoing allogeneic stem cell transplant. T cell depletion has been recognized as a method of reducing the incidence of GVHD in allogeneic transplants. Until recently, most T cell depletion methods were non-selective in reducing lymphocytes. Rhodamine purging is one method, which selectively reduces alloreactive T cells preventing GVHD. We review here the methods of non-selective and selective T cell depletion, particularly the newer method of photodynamic purging utilizing rhodamine.
Goggins
Timothy F
TF
Hematology-Oncology, Duke University Medical Center, 2400 Pratt Street, Ste. 1100, Durham, NC 27710, USA. goggi002@mc.duke.edu
Chao
Nelson
N
D000818:Animals; D006086:Graft vs Host Disease / Q000517:prevention & control*; D020544:Graft vs Leukemia Effect; D006801:Humans; D008212:Lymphocyte Depletion; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000494:pharmacology*; D012235:Rhodamines / Q000494:pharmacology*; D033581:Stem Cell Transplantation; D013601:T-Lymphocytes / Q000187:drug effects*; D014181:Transplantation Immunology; D014184:Transplantation, Homologous
D017319:Photosensitizing Agents; D012235:Rhodamines; C121365:4,5-dibromorhodamine 123