title: Crosstalk between the EGFR and LIN-12/Notch pathways in C. elegans vulval development.
abstract: The Caenorhabditis elegans vulva is an important paradigm for cell-cell interactions in animal development. The fates of six vulval precursor cells are patterned through the action of the epidermal growth factor receptor-mitogen-activated protein kinase (EGFR-MAPK) inductive signaling pathway, which specifies the 1 degrees fate, and the LIN-12/Notch lateral signaling pathway, which specifies the 2 degrees fate. Here, we provide evidence that the inductive signal is spatially graded and initially activates the EGFR-MAPK pathway in the prospective 2 degrees cells. Subsequently, this effect is counteracted by the expression of multiple new negative regulators of the EGFR-MAPK pathway, under direct transcriptional control of the LIN-12-mediated lateral signal.
authors.affiliation: Integrated Program in Cellular, Molecular, and Biophysical Studies, Howard Hughes Medical Institute, Columbia University, College of Physicians and Surgeons, 701 West 168th Street, Room 720, New York, NY 10032, USA.
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Yoo
authors.forename: Carlos
authors.initials: C
authors.lastname: Bais
authors.forename: Iva
authors.initials: I
authors.lastname: Greenwald
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D017238:Genes, Helminth; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D007763:Lac Operon; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D020935:MAP Kinase Signaling System; D008565:Membrane Proteins / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D008954:Models, Biological; D009024:Morphogenesis; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism; D051880:Receptors, Notch; D011993:Recombinant Fusion Proteins; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D014158:Transcription, Genetic; D014844:Vulva / Q000166:cytology / Q000254:growth & development / Q000378:metabolism
chemical_list: D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; C105157:Egl-17 protein, C elegans; D036341:Intercellular Signaling Peptides and Proteins; C082460:Lin-12 protein, C elegans; D008164:Luminescent Proteins; D008565:Membrane Proteins; D051880:Receptors, Notch; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D066246:ErbB Receptors; C085873:let-23 protein, C elegans; D020928:Mitogen-Activated Protein Kinases; C421904:lip-1 protein, C elegans; D017027:Protein Tyrosine Phosphatases