title: Evaluation of a urine antibody test for Helicobacter pylori in Japanese children.
abstract: OBJECTIVE
  To evaluate the accuracy of a urine-based enzyme-linked immunosorbent assay (ELISA) kit for anti-Helicobacter pylori immunoglobulin G antibody (urine-HpELISA) in children, we compared its sensitivity and specificity in reference to (13)C-urea-breath test (UBT) and H pylori stool antigen test (HpSA).
  
  
  METHODS
  Japanese children without significant upper abdominal symptoms were included (n=100; mean age, 7.0 years; range, 2 to 15). UBT, HpSA, and urine-HpELISA were performed.
  
  
  RESULTS
  Of 100 children, 36 and 64 were judged H pylori-positive and H pylori-negative, respectively, by UBT and HpSA. Thirty-four of 36 positive children were positive by urine-HpELISA, and 62 out of 64 negative children were negative by urine-HpELISA. Thus, the urine-HpELISA had 94.4% sensitivity and 96.9% specificity, with accuracy of 96.0%.
  
  
  CONCLUSIONS
  The urine-HpELISA is a rapid, inexpensive, reliable, and easy-to-perform method for the diagnosis of H pylori infection in children. It may be useful not only for diagnosis but also for mass screening for epidemiological studies in pediatric population.
authors.affiliation: Department of Pediatrics, Wakayama Rosai Hospital, Nachi-Katsuura Hot-Spring Hospital, Wakayama, Japan. okuda@naxnet.or.jp
authors.forename: Masumi
authors.initials: M
authors.lastname: Okuda
authors.forename: Teruko
authors.initials: T
authors.lastname: Nakazawa
authors.forename: Mina
authors.initials: M
authors.lastname: Booka
authors.forename: Eikichi
authors.initials: E
authors.lastname: Miyashiro
authors.forename: Norishige
authors.initials: N
authors.lastname: Yosikawa
mesh_terms: D000293:Adolescent; D000907:Antibodies, Bacterial / Q000652:urine*; D000942:Antigens, Bacterial / Q000032:analysis; D001944:Breath Tests; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005188:False Negative Reactions; D005189:False Positive Reactions; D005243:Feces / Q000382:microbiology; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology / Q000652:urine*; D007564:Japan; D008297:Male; D012680:Sensitivity and Specificity; D014508:Urea
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D014508:Urea