Proteomic fingerprinting of HIV-1-infected human monocyte-derived macrophages: a preliminary report.
Mononuclear phagocytes (MP; blood monocytes, alveolar, lymph node, and brain macrophages and microglia) are vehicles for dissemination and principle target cells for human immunodeficiency virus type 1 (HIV-1) infection. Notably, viral persistence in macrophages occurs despite ongoing phagocytic, intracellular killing, innate and adaptive immune responses. To assess potential pathways for how HIV-1 may bypass antiviral MP responses, we used proteomic tests to evaluate protein fingerprints of HIV-1-infected human monocyte-derived macrophages 7 days after viral infection. By using weak cation exchange chips, 58 proteins were found up- or down-regulated after HIV-1(ADA) infection. Several of these proteins were identified by microsequencing. It is probable that cellular proteins identified by proteomic fingerprinting could assist in unraveling how persistent viral infection occurs in MP lineage cells. Moreover, this evolving technology can be utilized to unravel changes in immune activities initiated by interactions between virus, environmental cues and drugs of abuse.
Carlson
Kimberly A
KA
Center for Neurovirology and Neurodegenerative Disorders, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-5215, USA.
Ciborowski
Pawel
P
Schellpeper
Courtney N
CN
Biskup
Toni M
TM
Shen
Rong Fong
RF
Luo
Xiaoguang
X
Destache
Christopher J
CJ
Gendelman
Howard E
HE
D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D015658:HIV Infections / Q000473:pathology*; D015497:HIV-1 / Q000472:pathogenicity; D006509:Hepatitis B / Q000097:blood; D006801:Humans; D008264:Macrophages / Q000737:chemistry / Q000378:metabolism* / Q000821:virology; D010449:Peptide Mapping / Q000379:methods*; D040081:Protein Array Analysis / Q000379:methods; D020543:Proteome; D020539:Sequence Analysis, Protein / Q000379:methods
D020543:Proteome