title: Sodium dodecyl sulfate-capillary gel electrophoresis of polyethylene glycolylated interferon alpha.
abstract: Sodium dodecyl sulfate-capillary gel electrophoresis (SDS-CGE) using a hydrophilic replaceable polymer network matrix was applied to characterize the polyethylene glycol(PEG)ylated interferon alpha (PEG-IFN). The SDS-CGE method resulted in a clearer resolution in both the PEG-IFN species and the native IFN species. The distribution profile of PEGylation determined by SDS-CGE was consistent with that obtained by SDS-polyacrylamide gel electrophoresis (PAGE) with Coomassie blue or barium iodide staining. The result was also compared using matrix-assisted laser desorption/ionization-time of flight-mass spectrometry. SDS-CGE was also useful for monitoring the PEGylation reaction to optimize the reaction conditions, such as reaction molar ratio. This study shows the potential of SDS-CGE as a new method for characterizing the PEGylated proteins with advantages of speed, minimal sample consumption and high resolution.
authors.affiliation: Drug Targeting Laboratory, College of Pharmacy, SungKyunKwan University, Suwon City, Korea.
authors.forename: Dong H
authors.initials: DH
authors.lastname: Na
authors.forename: Eun J
authors.initials: EJ
authors.lastname: Park
authors.forename: Yu S
authors.initials: YS
authors.lastname: Youn
authors.forename: Byung W
authors.initials: BW
authors.lastname: Moon
authors.forename: Yeong W
authors.initials: YW
authors.lastname: Jo
authors.forename: Sung H
authors.initials: SH
authors.lastname: Lee
authors.forename: Won-Bae
authors.initials: WB
authors.lastname: Kim
authors.forename: Yeowon
authors.initials: Y
authors.lastname: Sohn
authors.forename: Kang C
authors.initials: KC
authors.lastname: Lee
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D016898:Interferon-alpha / Q000032:analysis*; D011092:Polyethylene Glycols / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D016898:Interferon-alpha; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols