Analysis of microsatellite instability and loss of heterozygosity in breast cancer with the use of a well characterized multiplex system.
Analysis of microsatellite instability (MI) and loss of heterozygosity (LOH) is recommended for screening patients with sporadic and hereditary malignancies. This study shows an application of a fluorescent hexaplex PCR system for microsatellite typing on A.L.F. DNA Sequencer (Pharmacia Biotech). This technique detects changes in microsatellites providing a time-efficient, reliable and accurate method for MI and LOH analyses. The Fragment Manager software was used for automated size calculation and quantitation of DNA fragments, enabling rapid and precise measurement of allelic ratios. We examined 70 breast cancer and 70 control DNA specimens, classified all the patterns of microsatellite alterations, and set up MI and LOH assessment criteria for the automated multiplex fluorescent method.
Powierska-Czarny
Jolanta
J
Department of Human Biology, The Ludwik Rydygier Medical University of Bydgoszcz, Bydgoszcz, Poland. jolanta_powierska@o2.pl
Miścicka-Sliwka
Danuta
D
Czarny
Jakub
J
Grzybowski
Tomasz
T
Wozniak
Marcin
M
Drewa
Gerard
G
Czechowicz
Włodzimierz
W
Sir
Jan
J
D001943:Breast Neoplasms / Q000235:genetics*; D043171:Chromosomal Instability; D004586:Electrophoresis; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction