title: Biodegradation of DNA and nucleotides to nucleosides and free bases.
abstract: Thirty-two different microorganisms were examined in order to check their ability to degrade an exogenous DNA. Bacteria from species: Stenotrophomonas maltophilia, Brevundimonas diminuta, Bacillus subtilis, Mycobacterium butyricum and fungus Fusarium moniliforme were capable to degrade DNA to nucleic bases or their derivatives. Degradation of DNA by S. maltophilia resulted in formation of free bases, such as hypoxanthine, thymine, uracil and xanthine. The optimum concentration of DNA seemed to be 3 mg ml(-1). The mode of degradation of DNA nucleotides depended on the type of nucleotide and its concentration, but nucleic bases or their derivatives were always formed at the end of the reaction process.
authors.affiliation: National Institute of Public Health, 30/34 Chelmska, 00725, Warszawa, Poland. hanna@il.waw.pl
authors.forename: Hanna
authors.initials: H
authors.lastname: Kruszewska
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Misicka
authors.forename: Urszula
authors.initials: U
authors.lastname: Chmielowiec
mesh_terms: D001673:Biodegradation, Environmental; D004247:DNA / Q000378:metabolism*; D003838:Deoxyadenine Nucleotides / Q000378:metabolism; D003843:Deoxycytidine Monophosphate / Q000378:metabolism; D003848:Deoxyguanine Nucleotides / Q000378:metabolism; D006868:Hydrolysis; D009705:Nucleosides / Q000378:metabolism*; D009711:Nucleotides / Q000378:metabolism*; D020615:Stenotrophomonas maltophilia / Q000254:growth & development / Q000378:metabolism
chemical_list: D003838:Deoxyadenine Nucleotides; D003848:Deoxyguanine Nucleotides; D009705:Nucleosides; D009711:Nucleotides; D003843:Deoxycytidine Monophosphate; C007257:2'-deoxyguanosine 5'-phosphate; D004247:DNA