Transplantation of primary satellite cells improves properties of reinnervated skeletal muscles.
Skeletal muscle demonstrates a force deficit after repair of injured peripheral nerves. We tested the hypothesis that transplantation of satellite cells into reinnervated rabbit tibialis anterior (TA) muscles improves their properties. Adult rabbits underwent transection and immediate suture of the common peroneal nerve. In order to provide an environment favorable for cell transplantation, TA were then made to degenerate by cardiotoxin injection, either immediately or after a 2-month delay, which is sufficient for muscle reinnervation. In both cases, the injured TA were transplanted with cultured satellite cells 5 days after induction of muscle degeneration. When cells were transferred immediately after nerve repair, drastic morphological and functional muscle alterations were observed. However, when the muscles were allowed to become reinnervated before cell transplantation, muscles were heavier and developed a significantly higher maximal force compared to denervated-reinnervated muscles. Thus, application of the cell therapy protocol improved properties of denervated muscles only when they were allowed to become innervated. These results, which represent the application of cell therapy to improve force recovery of reinnervated muscles, will be of significant interest in certain clinical contexts, particularly after immediate or delayed muscle reinnervation.
Lazerges
Cyril
C
UMR 866 Différenciation Cellulaire et Croissance, Institut National de la Recherche Agronomique (INRA), 2 Place Pierre Viala, 34060 Montpellier, France.
Daussin
Paul-André
PA
Coulet
Bertrand
B
Boubaker el Andalousi
Ramzi
R
Micallef
Jean-Paul
JP
Chammas
Michel
M
Reyne
Yves
Y
Bacou
Francis
F
D000818:Animals; D017690:Cell Transplantation / Q000379:methods; D008297:Male; D009121:Muscle Denervation / Q000379:methods; D018482:Muscle, Skeletal / Q000294:innervation* / Q000502:physiology / Q000601:surgery*; D011817:Rabbits; D032496:Satellite Cells, Skeletal Muscle / Q000502:physiology / Q000637:transplantation*