title: A role for thyroid hormone in wound healing through keratin gene expression.
abstract: The importance of thyroid hormone (TH) in wound healing is not well understood. To gain insight, we evaluated the impact of TH deficiency on wound-healing genes in cultured keratinocytes. By RT-PCR, keratin 6a (K6a) and 16 (K16) gene expression in TH replete cells was 3.8- (P < 0.005) and 1.9-fold (P < 0.05) greater, respectively, than expression in TH-deficient cells. By real-time PCR, TH replete cell expression of K6a, K16, and K17 was greater than in deficient cells: 18- (P < 0.001), 10- (P < 0.001), and 4-fold (P < 0.005), respectively. To examine TH requirement for optimal wound healing, we contrasted TH-deficient vs. ip T(3)-treated mice. Four days after wounding, ip T(3)-treated mice had twice the degree of wound closure as hypothyroid mice (P < 0.001). By RT-PCR, K6a and K17 gene expression from control mouse skin was greater than from hypothyroid mouse skin: 5- (P < 0.001) and 1.7-fold (P < 0.05), respectively. T(3) is necessary for the keratinocyte proliferation required for optimal wound healing. T(3) exerts influence by stimulating expression of the wound-healing keratin genes. Thus, for hypothyroid patients undergoing surgery that cannot be delayed until euthyroidism is achieved, our data support T(3) treatment for the perioperative period.
authors.affiliation: Section of Endocrinology, Diabetes, and Nutrition, Boston University School of Medicine, 715 Albany Street, Room M-1022, Boston, Massachusetts 02118, USA. jsafer@bu.edu
authors.forename: Joshua D
authors.initials: JD
authors.lastname: Safer
authors.forename: Tara M
authors.initials: TM
authors.lastname: Crawford
authors.forename: Michael F
authors.initials: MF
authors.lastname: Holick
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D007037:Hypothyroidism / Q000378:metabolism; D015603:Keratinocytes / Q000737:chemistry / Q000166:cytology; D007633:Keratins / Q000235:genetics* / Q000502:physiology*; D051379:Mice; D016133:Polymerase Chain Reaction; D013965:Thyroidectomy; D014284:Triiodothyronine / Q000008:administration & dosage / Q000502:physiology*; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology*
chemical_list: D014284:Triiodothyronine; D007633:Keratins