title: Toxicovigilance: new biochemical tool used in sulfonylurea herbicides toxicology studies.
abstract: In vitro toxic effects of sulfonylurea herbicides (thifensulfuron-methyl and metsulfuron-methyl) were evaluated according to a new protocol. Physiological conditions were reproduced in order to boost toxicovigilance. Sulfonylureas and their hydrolysis products were added to biological substrates such as urea, alanine, aspartic acid, alpha-ketoglutarate, oxaloacetate, pyruvate and then incubated with some specific enzymes. Addition of these sulfonylureas and their degradation products did not significantly change the enzymatic activity of the urease, aspartate-aminotransferase, glutamate dehydrogenase, malate dehydrogenase and lactate dehydrogenase. However, the acid hydrolysis products inhibited up to 95% of the activity of the alanine-aminotransferase at low concentrations (0.27 micromol L(-1)). Inhibition did not affect the mitochondrial aspartate-aminotransferase.
authors.affiliation: Poison Control Center and Department of Toxicology, Purpan University Hospital Toulouse, France. belhadj.h@chu-toulouse.fr
authors.forename: Hafid
authors.initials: H
authors.lastname: Belhadj-Tahar
authors.forename: Nicolas
authors.initials: N
authors.lastname: Adamczewski
authors.forename: Bertrand
authors.initials: B
authors.lastname: Nassar
authors.forename: Yvon
authors.initials: Y
authors.lastname: Coulais
mesh_terms: D000409:Alanine / Q000378:metabolism; D000410:Alanine Transaminase / Q000378:metabolism; D000594:Amino Acid Oxidoreductases / Q000378:metabolism; D001190:Arylsulfonates / Q000737:chemistry / Q000633:toxicity; D002460:Cell Line; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D006540:Herbicides / Q000737:chemistry / Q000633:toxicity*; D006801:Humans; D006868:Hydrolysis; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008111:Liver Function Tests; D008291:Malate Dehydrogenase / Q000378:metabolism; D013453:Sulfonylurea Compounds / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D013876:Thiophenes / Q000737:chemistry / Q000633:toxicity; D014510:Urease / Q000378:metabolism
chemical_list: D001190:Arylsulfonates; D006540:Herbicides; D013453:Sulfonylurea Compounds; D013876:Thiophenes; C050296:metsulfuron methyl; C414314:thifensulfuron methyl; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D000594:Amino Acid Oxidoreductases; C025745:aspartate dehydrogenase; D005969:Glutamate Dehydrogenase; D000410:Alanine Transaminase; D014510:Urease; D000409:Alanine