Expression of axon guidance molecules and their related genes during development and sexual differentiation of the olfactory bulb in rats.
Axon guidance molecules and related proteins such as semaphorin 3A, neuropilin-1, plexin-1, netrin-1, growth-associated protein, olfactory marker protein, cypin and collapsin response mediator proteins guide the development of neural circuits in the olfactory bulb. In this study, transcriptions of these genes were examined in the olfactory bulb of female, male and neonatal testosterone propionate-treated female rats at the ages of 2, 5, 10, 15, 20, 25, 30 and 45 days. The semaphorin 3A, neuropilin-1, growth-associated protein and collapsin response mediator protein 1-5 genes were expressed significantly higher during the early development stages than in adulthood while the opposite is true for the olfactory marker protein. The expression profile of cypin and netrin-1 was relatively constant through development. A late effect of the neonatal testosterone propionate treatment on netrin-1, growth-associated protein, olfactory marker protein, collapsin response mediator proteins 1, 3, 4 and cypin gene expression was observed. The expression profiles of collapsin response mediator proteins and their related genes in the developing olfactory bulb confirmed most studies on the relationship between collapsin response mediator proteins and development in the brain. Sex differences of semaphorin 3A, neuropilin-1 as well as collapsin response mediator protein 3 at the early development stage and the late effect of neonatal testosterone propionate treatment on the expressions of netrin-1, growth-associated marker protein, cypin and collapsin response mediator proteins 1, 3 and 5 genes may indicate a possible role of these molecules on sexual differentiation of the olfactory bulb.
Tsim
T Y
TY
Department of Physiology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong.
Wong
E Y K
EY
Leung
M S
MS
Wong
C-C
CC
D048868:Adaptor Proteins, Signal Transducing; D000375:Aging; D000704:Analysis of Variance; D000818:Animals; D001369:Axons / Q000378:metabolism*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D005260:Female; D019922:GAP-43 Protein / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006148:Guanine Deaminase / Q000235:genetics / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D000075388:Netrin-1; D039942:Neuropilin-1 / Q000235:genetics / Q000378:metabolism; D009830:Olfactory Bulb / Q000254:growth & development* / Q000378:metabolism*; D051842:Olfactory Marker Protein; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D039961:Semaphorins / Q000235:genetics / Q000378:metabolism; D012733:Sex Differentiation / Q000502:physiology*; D025521:Tumor Suppressor Proteins; D000098855:Collapsin Response Mediator Protein 1
D048868:Adaptor Proteins, Signal Transducing; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; C485780:DPYSL4 protein, human; C449760:Dpysl3 protein, rat; C485779:Dpysl4 protein, rat; C413755:Dpysl5 protein, rat; D019922:GAP-43 Protein; D036341:Intercellular Signaling Peptides and Proteins; C000617351:NTN1 protein, human; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; C000617354:Ntn1 protein, rat; D051842:Olfactory Marker Protein; C497599:Omp protein, rat; D010750:Phosphoproteins; D012333:RNA, Messenger; D039961:Semaphorins; D025521:Tumor Suppressor Proteins; D000098855:Collapsin Response Mediator Protein 1; C095114:collapsin response mediator protein-2; C091529:plexin; D039942:Neuropilin-1; D000075388:Netrin-1; C485728:Gda protein, rat; D006148:Guanine Deaminase