title: Splicing enhances translation in mammalian cells: an additional function of the exon junction complex.
abstract: In mammalian cells, spliced mRNAs yield greater quantities of protein per mRNA molecule than do otherwise identical mRNAs not made by splicing. This increased translational yield correlates with enhanced cytoplasmic polysome association of spliced mRNAs, and is attributable to deposition of exon junction complexes (EJCs). Translational stimulation can be replicated by tethering the EJC proteins Y14, Magoh, and RNPS1 or the nonsense-mediated decay (NMD) factors Upf1, Upf2, and Upf3b to an intronless reporter mRNA. Thus, in addition to its previously characterized role in NMD, the EJC also promotes mRNA polysome association. Furthermore, the ability to stimulate translation when bound inside an open reading frame appears to be a general feature of factors required for NMD.
authors.affiliation: Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA.
authors.forename: Ajit
authors.initials: A
authors.lastname: Nott
authors.forename: Hervé
authors.initials: H
authors.lastname: Le Hir
authors.forename: Melissa J
authors.initials: MJ
authors.lastname: Moore
mesh_terms: D000818:Animals; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D046911:Macromolecular Substances; D014176:Protein Biosynthesis / Q000502:physiology*; D022821:RNA Splice Sites / Q000502:physiology*; D012326:RNA Splicing / Q000502:physiology*; D012333:RNA, Messenger / Q000502:physiology*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000378:metabolism; D014981:Xenopus
chemical_list: D046911:Macromolecular Substances; D022821:RNA Splice Sites; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell