Tomato paste fraction inhibiting the formation of advanced glycation end-products.
A water-soluble and low-molecular-weight fraction (SB) was obtained from tomato paste. The effects of SB on the formation of advanced glycation end-products (AGE) in protein glycation were studied by the methods of specific fluorescence, ELISA and a Western blot analysis, using the anti-AGE antibody after incubating protein with sugar. The results suggest that SB had strong inhibitory activity, in comparison with aminoguanidine as a positive control, and that the inhibitory mechanism of SB differed from that of aminoguanidine to involve trapping of reactive dicarbonyl intermediates in the early stage of glycation. SB contained an antioxidant, rutin, which showed potent inhibitory activity. The results also suggest that rutin chiefly contributed to inhibiting the formation of AGE, and that other compounds in SB may also have been related to the activity.
Kiho
Tadashi
T
Gifu Prefectural Institute of Health and Environmental Sciences, Gifu, Japan. kiho-tadashi@pref.gifu.lg.jp
Usui
Shigeyuki
S
Hirano
Kazuyuki
K
Aizawa
Koichi
K
Inakuma
Takahiro
T
D001120:Arginine / Q000031:analogs & derivatives* / Q000032:analysis / Q000494:pharmacology; D015153:Blotting, Western; D005591:Chemical Fractionation; D004797:Enzyme-Linked Immunosorbent Assay; D005502:Food; D005947:Glucose / Q000378:metabolism; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D018551:Solanum lycopersicum; D008239:Lysine / Q000031:analogs & derivatives* / Q000032:analysis / Q000494:pharmacology; D008970:Molecular Weight; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D009646:Norleucine / Q000031:analogs & derivatives* / Q000032:analysis; D010084:Oxidation-Reduction; D011758:Pyrroles / Q000032:analysis; D012431:Rutin / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D017127:Glycation End Products, Advanced; D011758:Pyrroles; D012710:Serum Albumin, Bovine; D009580:Nitroblue Tetrazolium; D012431:Rutin; C048496:N(6)-carboxymethyllysine; C059571:2-formyl-5-(hydroxymethyl)pyrrole-1-norleucine; D009646:Norleucine; D001120:Arginine; C062187:pentosidine; D005947:Glucose; D008239:Lysine