title: MATE transposable elements in Aspergillus nidulans: evidence of repeat-induced point mutation.
abstract: The sequences of five MATE transposable elements were retrieved from the Aspergillus nidulans genome sequence. These elements are 6.1 kb in length and are characterized by 9-10 bp target site duplications, paired approximately 40 bp palindromes close to each end, and in the unmutated elements, 57 clustered Spe-motifs (RWCTAGWY) scattered through their length. Short open reading frames have no known homology. Two of the MATE elements have numerous C --> T transitions on both DNA strands relative to the remaining three elements. These mutations have all the characteristics of repeat-induced point mutation (RIP) previously described in Neurospora crassa, but not experimentally demonstrated in A. nidulans. Ninety-eight percent of mutated cytosines are in CpG and CpA doublets, the former mutating at higher frequency.
authors.affiliation: Division of Molecular Genetics, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G11 6NU, Scotland, UK. j.clutterbuck@bio.gla.ac.uk
authors.forename: A John
authors.initials: AJ
authors.lastname: Clutterbuck
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D017354:Point Mutation; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004251:DNA Transposable Elements