Selective in vitro antioxidant properties of bisphosphonates.
The aim of this study was to investigate the in vitro antioxidant profile of different bisphosphonates. Bisphosphonates were tested for their xanthine oxidase and microsomal lipid peroxidation inhibiting capacity. Furthermore, the effect of these different compounds on DPPH, a stable radical, was investigated. Clodronate, risedronate, and pyrophosphate were further tested for their hydroxyl radical scavenging activity. None of the tested compounds showed xanthine oxidase inhibiting activity or DPPH scavenging activity. All the tested bisphosphonates exhibited inhibiting capacities on the microsomal lipid peroxidation. The hydroxyl radical scavenging activity was dependent on the order of adding the different reagents and was highest for risedronate. Bisphosphonates possess an inhibiting activity on the microsomal lipid peroxidation and the Fenton reaction. In these reactions iron plays an important role suggesting that the selective in vitro antioxidant properties of the bisphosphonates are due to their iron chelating characteristics.
Dombrecht
E J
EJ
Laboratory of Immunology, Allergology and Rheumatology, Department of Medicine, University of Antwerp, Universiteitsplein 1, B-2610, Antwerp, Belgium.
Cos
P
P
Vanden Berghe
D
D
Van Offel
J F
JF
Schuerwegh
A J
AJ
Bridts
C H
CH
Stevens
W J
WJ
De Clerck
L S
LS
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001713:Biphenyl Compounds; D011756:Diphosphates / Q000494:pharmacology; D004164:Diphosphonates / Q000737:chemistry / Q000494:pharmacology*; D005227:Fatty Acids / Q000737:chemistry / Q000494:pharmacology; D005296:Ferrous Compounds / Q000737:chemistry; D016166:Free Radical Scavengers / Q000494:pharmacology; D005609:Free Radicals / Q000037:antagonists & inhibitors; D017665:Hydroxyl Radical / Q000032:analysis / Q000037:antagonists & inhibitors / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D008862:Microsomes, Liver / Q000378:metabolism; D010851:Picrates / Q000037:antagonists & inhibitors; D051381:Rats; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors
D000975:Antioxidants; D001713:Biphenyl Compounds; D011756:Diphosphates; D004164:Diphosphonates; D005227:Fatty Acids; D005296:Ferrous Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D010851:Picrates; C013584:lipid peroxidation inhibitor; D017665:Hydroxyl Radical; C020748:ferrous sulfate; C004931:1,1-diphenyl-2-picrylhydrazyl; D014969:Xanthine Oxidase