title: Isolation of functional RNA from small amounts of different grape and apple tissues.
abstract: An efficient, simple, and small-scale procedure for isolating functional ribonucleic acid (RNA) was successfully applied to many different tissues of grape and apple. These woody plants are rich in polyphenolic compounds and polysaccharides that could impair the RNA extraction. The method chosen is based on the use of hot borate buffer at alkaline pH supplemented with several adjuvants and followed by selective precipitations. Starting with only 0.4 g of fresh tissue and working with small tubes (2 mL), we were able to obtain good yields of high-quality RNA suitable for further applications. The procedure can be proposed for many applications, and it is particularly highly recommended when isolating RNA from a large number of samples.
authors.affiliation: Istituto Agrario di S. Michele all'Adige, I-38010, S. Michele a/Adige, Trento-Italy. claudio.moser@mail.ismaa.it
authors.forename: Claudio
authors.initials: C
authors.lastname: Moser
authors.forename: Pamela
authors.initials: P
authors.lastname: Gatto
authors.forename: Mirko
authors.initials: M
authors.lastname: Moser
authors.forename: Massimo
authors.initials: M
authors.lastname: Pindo
authors.forename: Riccardo
authors.initials: R
authors.lastname: Velasco
mesh_terms: D011232:Chemical Precipitation; D027845:Malus / Q000235:genetics*; D008832:Microchemistry / Q000379:methods*; D010936:Plant Extracts / Q000235:genetics* / Q000302:isolation & purification*; D018749:RNA, Plant / Q000302:isolation & purification*; D014461:Ultracentrifugation / Q000379:methods*; D027843:Vitis / Q000235:genetics*
chemical_list: D010936:Plant Extracts; D018749:RNA, Plant