title: Improved survival of orthotopic liver allograft in swine by addition of trophic factors to University of Wisconsin solution.
abstract: Serum-free preservation media such as University of Wisconsin (UW) may cause tissue damage through trophic factor (TF) deprivation. This study evaluated whether the addition of TFs to UW solution improves liver graft quality after extended cold preservation time in pigs. UW solution was supplemented with epidermal growth factor, insulin-like growth factor-1, nerve growth factor-beta, bactenecin, and substance P to create TF-supplemented (TFS) UW. Orthotopic liver transplantation was performed after 18 hr of static cold storage at 4 degrees C in UW (n=7) or TFS-UW (n=7) solution. Recipients of grafts preserved with TFS-UW demonstrated significantly better 5-day survival (57%) than those preserved with UW alone (14%) (P<0.05). Adenosine triphosphate content in grafts preserved in TFS-UW was significantly higher than in grafts preserved in UW (17.4+/-5.0 vs. 4.8+/-1.2 nmol/mg protein, respectively) (P<0.05). This study showed that the addition of TFs to UW solution allowed a significant extension of cold ischemic time in pigs.
authors.affiliation: Laboratory of Experimental Surgery, Faculté de Médecine, Université Catholique de Louvain, Brussels, Belgium.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ambiru
authors.forename: Kenji
authors.initials: K
authors.lastname: Uryuhara
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Talpe
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Dehoux
authors.forename: Louise
authors.initials: L
authors.lastname: Jacobbi
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Murphy
authors.forename: Jonathan F
authors.initials: JF
authors.lastname: McAnulty
authors.forename: Pierre
authors.initials: P
authors.lastname: Gianello
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005978:Glutathione; D006085:Graft Survival / Q000187:drug effects / Q000502:physiology*; D006133:Growth Substances / Q000494:pharmacology*; D007328:Insulin; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D008099:Liver / Q000187:drug effects; D016031:Liver Transplantation / Q000502:physiology*; D023421:Models, Animal; D020932:Nerve Growth Factor / Q000494:pharmacology; D019188:Organ Preservation Solutions; D010456:Peptides, Cyclic / Q000494:pharmacology; D011887:Raffinose; D013373:Substance P / Q000494:pharmacology; D013552:Swine
chemical_list: D006133:Growth Substances; D007328:Insulin; D019188:Organ Preservation Solutions; D010456:Peptides, Cyclic; C055206:University of Wisconsin-lactobionate solution; C056454:bactenecin; D013373:Substance P; D004815:Epidermal Growth Factor; D000493:Allopurinol; D007334:Insulin-Like Growth Factor I; D020932:Nerve Growth Factor; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose