Specific detection of the toxigenic species Fusarium proliferatum and F. oxysporum from asparagus plants using primers based on calmodulin gene sequences.
Fusarium proliferatum and Fusarium oxysporum are the causal agents of a destructive disease of asparagus called Fusarium crown and root rot. F. proliferatum from asparagus produces fumonisin B1 and B2, which have been detected as natural contaminants in infected asparagus plants. Polymerase chain reaction (PCR) assays were developed for the rapid identification of F. proliferatum and F. oxysporum in asparagus plants. The primer pairs are based on calmodulin gene sequences. The PCR products from F. proliferatum and F. oxysporum were 526 and 534 bp long, respectively. The assays were successfully applied to identify both species from the vegetative part of the plants.
Mulè
Giuseppina
G
Institute of Sciences of Food Production, CNR, Viale Einaudi, 51, 70125 Bari, Italy. giuseppina.mule@ispa.cnr.it
Susca
Antonia
A
Stea
Gaetano
G
Moretti
Antonio
A
D027761:Asparagus Plant / Q000382:microbiology*; D001483:Base Sequence; D002147:Calmodulin / Q000235:genetics*; D017931:DNA Primers; D005670:Fusarium / Q000302:isolation & purification* / Q000472:pathogenicity*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity
D002147:Calmodulin; D017931:DNA Primers