Lack of association between human G-protein beta3 subunit variant and overweight in Japanese workers.
We examined the association of the G-protein beta3 subunit gene (GNB3) C825T polymorphism with overweight in Japanese workers. This cross-sectional study used multivariate analysis to investigate whether a polymorphism in the C825T polymorphism was associated independently with overweight when factors such as age and lifestyle were taken into account. The study in 1453 men and 1172 women involved identifying subjects with the C825T genotype using the polymerase chain reaction, followed by restriction fragment-length polymorphism analysis. Overweight was defined as a BMI > or = kg/m(2). Genotype distributions for C825T in overweight men (CC = 80, CT = 162, TT = 80) and women (CC = 52, CT = 91, TT = 40) were not significantly different from normal-weight men (CC = 278, CT = 588, TT = 265) and women (CC = 242, CT = 549, TT = 198). The allele distributions were also not significantly different between either sex. The power of the study was estimated as 98% in men and 81% in women based on the allelic frequencies reported in a previous positive study in Chinese subjects. Multiple logistic regression analysis showed that the genotype was not significantly associated with overweight. In conclusion, this study indicated that the GNB3 C825T polymorphism is not a significant factor for overweight in Japanese people.
Suwazono
Yasushi
Y
Department of Occupational and Environmental Medicine, Graduate School of Medicine, Chiba University, Chiba, Japan. suwa@faculty.chiba-u.jp
Okubo
Yasushi
Y
Kobayashi
Etsuko
E
Miura
Katsuyuki
K
Morikawa
Yuko
Y
Ishizaki
Masao
M
Kido
Teruhiko
T
Nakagawa
Hideaki
H
Nogawa
Koji
K
D000328:Adult; D000704:Analysis of Variance; D015992:Body Mass Index; D003430:Cross-Sectional Studies; D005260:Female; D005838:Genotype; D020962:Heterotrimeric GTP-Binding Proteins / Q000235:genetics*; D006801:Humans; D007564:Japan; D008019:Life Style; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
C454420:G-protein beta3 subunit; D020962:Heterotrimeric GTP-Binding Proteins