Development and validation of a reversed-phase liquid chromatographic method for analysis of D4T (Stavudine) in rat plasma.
An improved and simplified high-performance liquid chromatographic (HPLC) method at UV detection 265 nm is presented for the determination of d4T in rat plasma. The mobile phase consists of methanol-distilled water-acetic acid in the 23:77:0.2 (v/v) ratio. Quantification is achieved by the peak-area ratio method with reference to the internal standard. This paper presents linearity, accuracy, precision, limit of quantification and limit of detection, specificity-selectivity and sample stability data. Based on the intra and inter-day validation, all coefficients of variation (CV) were found less than 15%. The assay is sufficiently rapid and sensitive and was applied in a pharmacokinetic study in rats.
Contreras
J
J
Department of Analytical Chemistry, HPLC and Pharmacokinetics Laboratory, National Center for Scientific Research, PO Box 6412/6414, Havana, Cuba. contreras@diramic.cneuro.edu.cu
González
H Ma
HM
Menéndez
R
R
López
M
M
D000818:Animals; D019380:Anti-HIV Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018119:Stavudine / Q000097:blood* / Q000493:pharmacokinetics
D019380:Anti-HIV Agents; D018119:Stavudine