title: Crystallization and preliminary crystallographic data of a leucotoxin S component from Staphylococcus aureus.
abstract: Class S proteins of staphylococcal bicomponent pore-forming leucotoxins play an important role in membrane targetting and cell specificity. Wild-type and recombinant S components of the Panton-Valentine leucocidin (LukS-PV) were expressed in Staphylococcus aureus and Escherichia coli, respectively, and purified. Both proteins were crystallized in two crystal forms with Jeffamine M-600 as the precipitant at 285 K using the hanging-drop vapour-diffusion method and seeding techniques. Crystals belong to space group P2 (or P2(1)) and P4(1) (or P4(3)), with unit-cell parameters a = 72.3, b = 95.1, c = 108.1 A, beta = 106.4 degrees and a = b = 94.8, c = 306.2 A, respectively. A full set of X-ray diffraction data was collected to 2.1 A from a single tetragonal crystal of the wild-type protein at 100 K.
authors.affiliation: Groupe de Biophysique Structurale, Département 'Mécanismes Moléculaires des Infections Mycobactériennes', IPBS-CNRS, 205 Route de Narbonne, 31077 Toulouse CEDEX, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Guillet
authors.forename: Daniel
authors.initials: D
authors.lastname: Keller
authors.forename: Gilles
authors.initials: G
authors.lastname: Prévost
authors.forename: Lionel
authors.initials: L
authors.lastname: Mourey
mesh_terms: D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism; D005098:Exotoxins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D013211:Staphylococcus aureus / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014961:X-Ray Diffraction
chemical_list: D005098:Exotoxins; D011994:Recombinant Proteins; C018579:leukotoxin