title: Catalytic Reaction of Basidiomycete Lentinula edodes Cytochrome P450, Le.CYP1 Enzyme Produced in Yeast.
abstract: The basidiomycete Lentinula edodes (Le.) cytochrome P450, Le.CYP1 was functionally expressed in Saccharomyces cerevisiae. The microsomal fraction containing Le.CYP1 was prepared from the recombinant yeast and the Le.CYP1 was analyzed. The 7-ethoxycoumarin and benzo(a)pyrene were found to be the substrates of Le.CYP1 enzyme. Le.CYP1 converted 7-ethoxycoumarin to 7-hydroxycoumarin.
authors.affiliation: Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
authors.forename: Ryoko
authors.initials: R
authors.lastname: Akiyama
authors.forename: Susumu
authors.initials: S
authors.lastname: Kajiwara
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shishido
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001564:Benzo(a)pyrene / Q000378:metabolism; D002384:Catalysis; D003374:Coumarins / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism*; D018076:DNA, Complementary; D008861:Microsomes / Q000378:metabolism; D008969:Molecular Sequence Data; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D020942:Shiitake Mushrooms / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D003374:Coumarins; D018076:DNA, Complementary; D011994:Recombinant Proteins; C017299:7-ethoxycoumarin; D001564:Benzo(a)pyrene; D003577:Cytochrome P-450 Enzyme System