[Correlation between secretion of VEGF and proliferation of cultured bovine retinal pigment epithelium cells under hypoxic condition].
OBJECTIVE
To investigate the effect of hypoxia on bovine retinal pigment epithelial (RPE) cell secreting vascular endothelial growth factor (VEGF) and the correlation between VEGF and proliferation of the cultured bovine retinal pigment epithelial cell.


METHODS
Cells we harvested were divided into different groups with or without anti-VEGF monoclonal antibody (VEGF MAb), and then grew under hypoxic and normal (as a control) condition. After 24 h, cell supernatants were collected and used for ELISA VEGF protein assay. The proliferation of cells grown under different conditions was detected by MTT colorimetric assay.


RESULTS
Cell proliferation and concentration of VEGF protein: The hypoxia with VEGF MAb group (Group A) was (1.812 +/- 0.068), (55.07 +/- 19.18) pg/ml; the hypoxia without VEGF MAb group (Group B) was (2.292 +/- 0.197), (171.61 +/- 16.02) pg/ml; the normal with VEGF MAb group (Group C) was (1.350 +/- 0.185), (43.92 +/- 21.39) pg/ml; the normal without VEGF MAb group (Group D) was (1.435 +/- 0.157), (48.51 +/- 24.73) pg/ml. Group A was lower than Group B on cell proliferation and concentration of VEGF protein. There is obvious difference between the two groups on both sides (P < 0.05). Group A was higher than Group D on cell proliferation. There is obvious difference between them on cell proliferation (P < 0.05). Group B is higher than Group D on cell proliferation and concentration of VEGF protein. There is obvious difference between them on both sides (P < 0.05). There is a linear association between the concentration of VEGF protein and the proliferation of RPE cell under hypoxic condition by Pearson Correlation (r = 1.000, P < 0.05).


CONCLUSIONS
Hypoxia stimulates the secretion of vascular endothelium growth factor of bovine RPE cell, which contributes to the proliferation of bovine RPE cell.
Li
Yunping
Y
Department of Ophthalmology, 2nd Affiliated Hospital of XiangYa Medical College, Centra South University, Changsha, Hunan 410011, China.
Tang
Luosheng
L
Jia
Songbai
S
D000818:Animals; D002417:Cattle; D002455:Cell Division; D015687:Cell Hypoxia; D002478:Cells, Cultured; D010857:Pigment Epithelium of Eye / Q000166:cytology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D042461:Vascular Endothelial Growth Factor A