title: Moment analysis of retention equilibrium, mass transfer kinetics, and thermodynamic properties in reversed-phase liquid chromatography using phenyl bonded silica gel.
abstract: Information about retention equilibrium, mass transfer kinetics, and related thermodynamic properties of chromatography using phenyldimethylsilyl (Ph)-silica gel was derived by moment analysis of pulse response peak profiles. The results for the Ph-silica gel were compared with those for octadecyldimethylsilyl (C18)-silica gel. Some parameters characterizing the chromatographic behavior of the two stationary phases were correlated with the hydrophobic surface area of sample molecules. Surface diffusion had a predominant role for intraparticle diffusion. An enthalpy-entropy compensation was established for both the retention equilibrium and surface diffusion. A linear correlation was observed between the logarithm of surface diffusion coefficient (Ds) and that of retention equilibrium constant (Ka), suggesting the establishment of a linear free energy relationship. The ratio of Ds to molecular diffusivity (Dm) decreased with increasing Ka and was correlated by a single curved line. The value of Ds was of the same order of magnitude with Dm when Ka became negligible. These results suggest the presence of a sort of correlation between surface diffusion and molecular diffusion and the restriction of the molecular mobility by surface diffusion due to the retention strength.
authors.affiliation: Material Science and Technology, Nagoya Institute of Technology, Gokiso, Showa, Nagoya 466-8555, Japan.
authors.forename: Kanji
authors.initials: K
authors.lastname: Miyabe
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Sakai
authors.forename: Mina
authors.initials: M
authors.lastname: Sarashina
authors.forename: Chie
authors.initials: C
authors.lastname: Yokokawa
mesh_terms: D000393:Air Pollutants / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D005782:Gels; D011083:Polycyclic Compounds / Q000032:analysis*; D012822:Silicon Dioxide
chemical_list: D000393:Air Pollutants; D005782:Gels; D011083:Polycyclic Compounds; D012822:Silicon Dioxide