Effect of endodontic agents on cytotoxicity induction by sodium fluoride.
We investigated six endodontic agents for their ability to induce apoptosis and modify the cytotoxic activity of NaF against human squamous cell carcinoma (HSC-2) and human promyelocytic leukemia (HL-60) cell lines. Four Group I agents (Form Cresol, Cam Phenic, Eucaly Soft, GC Fuji Varnish), but not two Group II agents (Caviton, Canals-N), induced internucleosomal DNA fragmentation and activated caspases 3, 8 and 9 in HL-60 cells. Only Cam Phenic among these agents additively enhanced the cytotoxic activity of NaF in HSC-2 and HL-60 cells. Form Cresol and Cam Phenic reduced the glucose consumption at early stage, possibly due to their toxic effect. Amino acid analysis suggests that the higher cytotoxicity of Form Cresol may be derived, at least in part, from its oxidizing action.
Tokunaga
Takashi
T
Department of Dental Pharmacology, Meikai University School of Dentistry, Sakado, Saitama, 350-0283, Japan.
Morshed
Sufi Reza Md
SR
Otsuki
Sumiko
S
Takayama
Fumitoshi
F
Hashimoto
Ken
K
Kashimata
Masanori
M
Nakamura
Yuko
Y
Nishikawa
Hirofumi
H
Yasui
Toshikazu
T
Yokote
Yoshiko
Y
Akahane
Kiso
K
Sakagami
Hiroshi
H
D017209:Apoptosis / Q000187:drug effects*; D002133:Calcium Sulfate / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9; D020169:Caspases / Q000378:metabolism; D003738:Dental Cements; D004338:Drug Combinations; D005947:Glucose / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute; D008715:Methionine / Q000378:metabolism; D009062:Mouth Neoplasms; D009910:Oral Hygiene; D010084:Oxidation-Reduction; D012387:Root Canal Filling Materials; D012969:Sodium Fluoride / Q000494:pharmacology*; D014753:Vinyl Compounds / Q000494:pharmacology; D015034:Zinc Oxide / Q000494:pharmacology
D003738:Dental Cements; D004338:Drug Combinations; D012387:Root Canal Filling Materials; D014753:Vinyl Compounds; C086076:calcium sulfate, zinc oxide, vinyl acetate, zinc phosphate root canal filling; D012969:Sodium Fluoride; D008715:Methionine; C505407:CASP3 protein, human; C505530:CASP8 protein, human; C506433:CASP9 protein, human; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9; D020169:Caspases; D005947:Glucose; D015034:Zinc Oxide; D002133:Calcium Sulfate