title: Increased expression of erythropoietin receptor on blood vessels in the human epileptogenic hippocampus with sclerosis.
abstract: Microvascular (capillary) proliferation is a readily observed, but largely ignored phenomenon of the mesial temporal lobe epilepsy (MTLE) hippocampus. Here, we report that the proliferated capillaries in surgically resected MTLE hippocampi were strongly immunoreactive for erythropoietin receptor (EPO-r). Further, we found that these capillaries were most prominent in areas of the MTLE hippocampus with extensive neuronal loss and gliosis, i.e. the CA3, CA1, and dentate hilus. High-resolution immunogold electron microscopy revealed that the capillary EPO-r was localized to the luminal and abluminal plasma membrane of endothelial cells, to endosome-like structures of these cells, and to pericapillary astrocytic end-feet. Previous studies have shown that systemically administered EPO appears in the cerebrospinal fluid in experimental animals, and the present results are consistent with the idea that EPO enters the brain via receptor-mediated endocytosis. The enrichment of EPO-r shown here suggests a highly efficient uptake of plasma EPO into the MTLE hippocampus and a possible role for this cytokine in epileptogenesis. Moreover, the presence of EPO-r in the MTLE hippocampus may provide a new vehicle for highly efficient delivery of hitherto impermeable drugs into the epileptic brain.
authors.affiliation: Department of Neurosurgery, Yale University School of Medicine, New Haven, Connecticut 06520-8082, USA. tore.eid@yale.edu
authors.forename: Tore
authors.initials: T
authors.lastname: Eid
authors.forename: Michael L
authors.initials: ML
authors.lastname: Brines
authors.forename: Anthony
authors.initials: A
authors.lastname: Cerami
authors.forename: Dennis D
authors.initials: DD
authors.lastname: Spencer
authors.forename: Jung H
authors.initials: JH
authors.lastname: Kim
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Schweitzer
authors.forename: Ole P
authors.initials: OP
authors.lastname: Ottersen
authors.forename: Nihal C
authors.initials: NC
authors.lastname: de Lanerolle
mesh_terms: D000328:Adult; D000368:Aged; D001253:Astrocytes / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D004730:Endothelium, Vascular / Q000378:metabolism; D004833:Epilepsy, Temporal Lobe / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006624:Hippocampus / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D008297:Male; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D017467:Receptors, Erythropoietin / Q000096:biosynthesis* / Q000648:ultrastructure; D012598:Sclerosis / Q000378:metabolism / Q000473:pathology
chemical_list: D017467:Receptors, Erythropoietin