Are combined analyses better than single gene phylogenies? A case study using SSU rDNA and rbcL sequence comparisons in the Zygnematophyceae (Streptophyta).
Although the combination of different genes in phylogenetic analyses is a promising approach, the methodology is not well established and analyses often suffer from inadequate, noncongruent taxon sampling, long-branch attraction, or conflicting evolutionary models of the genes analyzed. Conflicts or congruence between multigene and single-gene phylogenies, as well as the assumed superiority of the multigene approach, are often difficult to assess solely because of incongruent taxon sampling. In the present study, a data set of 43 nuclear-encoded SSU rDNA and plastid-encoded rbcL gene sequences was generated from the same strains of conjugating green algae (Zygnematophyceae, Streptophyta). Phylogenetic analyses used the genes individually and in combination, either as concatenated sequences or with the log-likelihood summation method. Single-gene analyses, although mostly congruent, revealed some conflicting nodes and showed different patterns of statistical support. Combined analyses confidently resolved the conflicts between the single-gene analyses, enhanced phylogenetic resolution, and were better supported by morphological information. Long-branch taxa were not the same for the two genes analyzed, and, thus, their effect on phylogenetic resolution was minimized in the combined analyses.
Gontcharov
Andrey A
AA
Botanisches Institut, Lehrstuhl I, Universität zu Köln, Köln, Germany.
Marin
Birger
B
Melkonian
Michael
M
D000460:Chlorophyta / Q000145:classification / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D019143:Evolution, Molecular; D016013:Likelihood Functions; D009841:Oligonucleotides / Q000235:genetics; D010802:Phylogeny; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
D004275:DNA, Ribosomal; D009841:Oligonucleotides; C101625:RbcL protein, plastid; D012273:Ribulose-Bisphosphate Carboxylase