Differential regulation of host cellular genes by HIV-1 viral protein R (Vpr): cDNA microarray analysis using isogenic virus.
HIV-1 Vpr is a protein with multiple functions. It has been suggested that such pleiotropic effects by a viral protein may be mediated by its association with viral and cellular proteins or through modulation of expression of specific cellular genes. To address this, we used cDNA microarray techniques to analyze the regulation of a panel of host cellular genes by HIV-1 Vpr using isogenic HIV-1 either with or without Vpr expression. Results indicate that Vpr downregulated the expression of genes involved in cell cycle/proliferation regulation, DNA repair, tumor antigens, and immune activation factors, and upregulated many ribosomal and structural proteins. These results for the first time reveal the involvement of several potential cellular genes, which may be useful, both for understanding Vpr functions and for the development of therapeutics targeting the Vpr molecule.
Janket
Michelle L
ML
Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, PA 15261, USA.
Manickam
Pachiappan
P
Majumder
Biswanath
B
Thotala
Dineshkumar
D
Wagner
Marc
M
Schafer
Elizabeth A
EA
Collman
Ronald G
RG
Srinivasan
Alagarsamy
A
Ayyavoo
Velpandi
V
D015153:Blotting, Western; D018076:DNA, Complementary; D005786:Gene Expression Regulation / Q000502:physiology*; D016336:Gene Products, vpr / Q000502:physiology*; D015497:HIV-1 / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054325:vpr Gene Products, Human Immunodeficiency Virus
D018076:DNA, Complementary; D016336:Gene Products, vpr; D054325:vpr Gene Products, Human Immunodeficiency Virus