title: Protein profile of Acidithiobacillus ferrooxidans strains exhibiting different levels of tolerance to metal sulfates.
abstract: Strains of Acidithiobacillus ferrooxidans exhibited differences in the inhibition of Fe2+ oxidation in the presence of 250 mM of cadmium, zinc, and manganese sulfates in respirometric assays. Strains LR and 135 were practically not inhibited, whereas strains SSP and V3 showed significant inhibition (30-70%). Analysis by SDS-PAGE of total proteins from cells grown in the absence of metal sulfates showed different profiles between the more tolerant strains (LR and 135) and the more susceptible ones (SSP and V3). Total proteins of strains LR and V3 were also resolved by two-dimensional polyacrylamide gel electrophoresis (2-DE). A set of major proteins (40, 32, 22, and 20 kDa) could be identified only in the more tolerant strain LR. Our results show that protein profiles analysis could differentiate A. ferrooxidans strains that considerably differ in the tolerance to metal sulfates and present low genomic similarity as revealed by Random Amplified Polymorphic DNA (RAPD) data obtained previously in our laboratory.
authors.affiliation: Departamento de Genética e Evolução, Centro de Ciências Biológicas e da Saúde, Universidade Federal de São Carlos, Rodovia Washington Luiz, km 235, 13565-905 São Carlos, SP, Brazil. marinovo@iq.unesp.br
authors.forename: Maria Teresa Marques
authors.initials: MT
authors.lastname: Novo
authors.forename: Oswaldo
authors.initials: O
authors.lastname: Garcia Júnior
authors.forename: Laura Maria Mariscal
authors.initials: LM
authors.lastname: Ottoboni
mesh_terms: D042763:Acidithiobacillus / Q000737:chemistry* / Q000145:classification / Q000187:drug effects* / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis*; D002104:Cadmium / Q000494:pharmacology; D004269:DNA, Bacterial / Q000032:analysis; D024881:Drug Resistance, Bacterial; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005296:Ferrous Compounds / Q000378:metabolism; D008345:Manganese / Q000494:pharmacology; D019216:Metals, Heavy / Q000494:pharmacology*; D010084:Oxidation-Reduction; D020543:Proteome / Q000032:analysis; D019105:Random Amplified Polymorphic DNA Technique; D015032:Zinc / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005296:Ferrous Compounds; D019216:Metals, Heavy; D020543:Proteome; D002104:Cadmium; D008345:Manganese; D015032:Zinc