Measurement of the malondialdehyde-2'-deoxyguanosine adduct in human urine by immuno-extraction and liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry.
The major adduct of malondialdehyde with guanine, M(1)G, was measured in human urine from non-smoking healthy individuals. M1G is a mutagenic DNA lesion and a terminal product of lipid peroxidation in vivo that may be implicated in cancer related to lifestyle and diet. On the basis of a recently developed method for the quantification of M1G as an excreted deoxynucleoside using immuno-extraction purification, chemical NaBH4 reduction and liquid chromatography combined with atmospheric pressure chemical ionization tandem mass spectrometry, we demonstrate that the average 24 h excretion rate of M1G-dR is about 12 +/- 3.8 fmol kg(-1) (n = 5).
Hoberg
Anne-Mette
AM
Department of Clinical Pharmacology, Q-7642, Rigshospitalet, Copenhagen University Hospital, DK-2100 Copenhagen Ø, Denmark.
Otteneder
Michael
M
Marnett
Lawrence J
LJ
Poulsen
Henrik E
HE
D000328:Adult; D001274:Atmospheric Pressure; D002853:Chromatography, Liquid; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification / Q000652:urine*; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008875:Middle Aged
C089188:3-(2'-deoxyribofuranosyl)pyrimido(1,2-a)purin-10(3H)-one; D003849:Deoxyguanosine