title: Inhibitory effects of manassantin A and B isolated from the roots of Saururus chinensis on PMA-induced ICAM-1 expression.
abstract: Cell adhesion inhibitors were isolated from the methanol extract of Saururus chinensis roots by bioactivity-guided fractionation. The active compounds were identified as manassantin A ( 1) and B ( 2), dineolignan compounds. Compounds 1 and 2 inhibited PMA-induced ICAM-1/LFA-1-mediated homotypic aggregation of the HL-60 cells without cytotoxicity with MIC values of 1.0 and 5.5 nM, respectively. Even though 1 and 2 did not affect the adhesion of ICAM-1 to LFA-1, these compounds inhibited PMA-induced ICAM-1 expression in HL-60 cells in a dose-dependent fashion. These results suggest that 1 and 2 inhibit cell aggregation through down-regulation of ICAM-1 expression.
authors.affiliation: Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology, Taejon, Korea.
authors.forename: Mun-Chual
authors.initials: MC
authors.lastname: Rho
authors.forename: Oh Eok
authors.initials: OE
authors.lastname: Kwon
authors.forename: Koanhoi
authors.initials: K
authors.lastname: Kim
authors.forename: Seung Woong
authors.initials: SW
authors.lastname: Lee
authors.forename: Mi Yeon
authors.initials: MY
authors.lastname: Chung
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
authors.forename: Masahiko
authors.initials: M
authors.lastname: Hayashi
authors.forename: Hyun Sun
authors.initials: HS
authors.lastname: Lee
authors.forename: Young-Kook
authors.initials: YK
authors.lastname: Kim
mesh_terms: D002449:Cell Aggregation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005663:Furans / Q000008:administration & dosage / Q000494:pharmacology*; D018922:HL-60 Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000187:drug effects* / Q000378:metabolism; D017705:Lignans; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D018517:Plant Roots; D032134:Saururaceae; D013755:Tetradecanoylphorbol Acetate
chemical_list: D005663:Furans; D017705:Lignans; D010936:Plant Extracts; C482885:manassantin B; D018799:Intercellular Adhesion Molecule-1; C054895:manassantin A; D013755:Tetradecanoylphorbol Acetate