Effect of Coriolis force on counter-current chromatographic separation by centrifugal partition chromatography.
The effect of Coriolis force on the counter-current chromatographic separation was studied using centrifugal partition chromatography (CPC) with four different two-phase solvent systems including n-hexane-acetonitrile (ACN); tert-butyl methyl ether (MtBE)-aqueous 0.1% trifluoroacetic acid (TFA) (1:1); MtBE-ACN-aqueous 0.1% TFA (2:2:3); and 12.5% (w/w) polyethylene glycol (PEG) 1000-12.5% (w/w) dibasic potassium phosphate. Each separation was performed by eluting either the upper phase in the ascending mode or the lower phase in the descending mode, each in clockwise (CW) and counterclockwise column rotation. Better partition efficiencies were attained by the CW rotation in both mobile phases in all the two-phase solvent systems examined. The mathematical analysis also revealed the Coriolis force works favorably under the CW column rotation for both mobile phases. The overall results demonstrated that the Coriolis force produces substantial effects on CPC separation in both organic-aqueous and aqueous-aqueous two-phase systems.
Ikehata
Jun-Ichi
J
Division of Analytical Chemistry, College of Pharmacy, Nihon University, 7-7-1, Narashinodai 7-Chome, Funabashi-shi, Chiba 274-8555, Japan.
Shinomiya
Kazufusa
K
Kobayashi
Koji
K
Ohshima
Hisashi
H
Kitanaka
Susumu
S
Ito
Yoichiro
Y
D002498:Centrifugation; D003377:Countercurrent Distribution / Q000379:methods*