title: The effects of single doses of beta radiation on the wound healing behaviour of human Tenon's capsule fibroblasts.
abstract: OBJECTIVE
  To determine the effects of single doses of beta radiation on the wound healing functions of human Tenon's capsule fibroblasts (hTf).
  
  
  METHODS
  hTf were grown in tissue culture and irradiated with beta radiation using a strontium 90 source. The effects of beta radiation on fibroblast migration was studied using microporous transwell membranes. The effects of radiation on fibroblast contraction was investigated using a fibroblast populated collagen gels model. Production of extracellular matrix molecules (collagen I, collagen III, and fibronectin) by monolayers of irradiated fibroblasts was quantified for 14 days following single doses of beta radiation.
  
  
  RESULTS
  Growth inhibiting doses of beta radiation did not inhibit fibroblast migration or contraction at any time point. Levels of soluble fibronectin from irradiated populations were significantly reduced after >500 cGy beta radiation. Collagen I and III levels were not reduced after any dose of radiation, and increased following treatment with 1000 cGy beta radiation.
  
  
  CONCLUSIONS
  Growth arresting doses of beta radiation have unique effects on the wound healing behaviour of human Tenon's capsule fibroblasts. There was no significant effect on cellular migration or contraction, but ECM production was altered. Fibronectin production was inhibited following higher radiation doses, and collagen I and III production increased after 1000 cGy. The effects of single doses of beta radiation on ocular fibroblast wound healing behaviour are very different from those of 5-fluorouracil and mitomycin C, and these differences may be exploited clinically in the regulation of wound healing after glaucoma filtration surgery.
authors.affiliation: International Collaborative Wound Healing Research Group, Institute of Ophthalmology and Moorfields Eye Hospital, London, UK. Hadley@ision.co.uk
authors.forename: P H
authors.initials: PH
authors.lastname: Constable
authors.forename: J G
authors.initials: JG
authors.lastname: Crowston
authors.forename: N L
authors.initials: NL
authors.lastname: Occleston
authors.forename: P T
authors.initials: PT
authors.lastname: Khaw
mesh_terms: D001610:Beta Particles; D002455:Cell Division / Q000528:radiation effects; D002465:Cell Movement / Q000528:radiation effects; D048429:Cell Size / Q000528:radiation effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis; D024061:Collagen Type III / Q000096:biosynthesis; D003239:Connective Tissue Cells / Q000378:metabolism / Q000528:radiation effects*; D017077:Culture Media, Conditioned; D004307:Dose-Response Relationship, Radiation; D005109:Extracellular Matrix / Q000378:metabolism / Q000528:radiation effects; D005123:Eye / Q000378:metabolism / Q000528:radiation effects*; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects*; D005353:Fibronectins / Q000096:biosynthesis; D018463:Filtering Surgery; D006801:Humans; D013326:Strontium Radioisotopes; D014945:Wound Healing / Q000528:radiation effects*
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D017077:Culture Media, Conditioned; D005353:Fibronectins; D013326:Strontium Radioisotopes