title: Stem cell-mediated muscle regeneration is enhanced by local isoform of insulin-like growth factor 1.
abstract: We investigated the mechanism whereby expression of a transgene encoding a locally acting isoform of insulin-like growth factor 1 (mIGF-1) enhances repair of skeletal muscle damage. Increased recruitment of proliferating bone marrow cells to injured MLC/mIgf-1 transgenic muscles was accompanied by elevated bone marrow stem cell production in response to distal trauma. Regenerating MLC/mIgf-1 transgenic muscles contained increased cell populations expressing stem cell markers, exhibited accelerated myogenic differentiation, expressed markers of regeneration and readily converted cocultured bone marrow to muscle. These data implicate mIGF-1 as a powerful enhancer of the regeneration response, mediating the recruitment of bone marrow cells to sites of tissue damage and augmenting local repair mechanisms.
authors.affiliation: Department of Histology and Medical Embryology and Centro Eccellenza Biologia e Medicina Molecolare, University of Rome "La Sapienza," 00161 Rome, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Musarò
authors.forename: Cristina
authors.initials: C
authors.lastname: Giacinti
authors.forename: Giovanna
authors.initials: G
authors.lastname: Borsellino
authors.forename: Gabriella
authors.initials: G
authors.lastname: Dobrowolny
authors.forename: Laura
authors.initials: L
authors.lastname: Pelosi
authors.forename: Linda
authors.initials: L
authors.lastname: Cairns
authors.forename: Sergio
authors.initials: S
authors.lastname: Ottolenghi
authors.forename: Giulio
authors.initials: G
authors.lastname: Cossu
authors.forename: Giorgio
authors.initials: G
authors.lastname: Bernardi
authors.forename: Luca
authors.initials: L
authors.lastname: Battistini
authors.forename: Mario
authors.initials: M
authors.lastname: Molinaro
authors.forename: Nadia
authors.initials: N
authors.lastname: Rosenthal
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology; D039481:CD11b Antigen / Q000032:analysis; D002465:Cell Movement; D007334:Insulin-Like Growth Factor I / Q000502:physiology*; D017493:Leukocyte Common Antigens / Q000032:analysis; D051379:Mice; D008822:Mice, Transgenic; D009132:Muscles / Q000502:physiology*; D020033:Protein Isoforms; D012038:Regeneration / Q000502:physiology*; D013234:Stem Cells / Q000502:physiology*
chemical_list: D039481:CD11b Antigen; D020033:Protein Isoforms; D007334:Insulin-Like Growth Factor I; D017493:Leukocyte Common Antigens