Creatine increases IGF-I and myogenic regulatory factor mRNA in C(2)C(12) cells.
Addition of creatine to the differentiation medium of C(2)C(12) cells leads to hypertrophy of the myotubes. To investigate the implication of insulin-like growth factor I (IGF-I) and myogenic regulatory factors (MRFs) in this hypertrophy, their mRNA levels were assessed during the first 72 h of differentiation. Creatine significantly increased the IGF-I mRNA level over the whole investigated period of time, whereas the MRF mRNA levels were only augmented at precise moments, suggesting a general activation mechanism for IGF-I and a specifically regulated mechanism for MRF transcription. Our results suggest therefore that creatine-induced hypertrophy of C(2)C(12) cells is at least partially mediated by overexpression of IGF-I and MRFs.
Louis
Magali
M
Département d'Education Physique et de Réadaptation, Université catholique de Louvain, Place Pierre de Coubertin 1, B-1348 Louvain-la-Neuve, Belgium. magali.louis@edph.ucl.ac.be
Van Beneden
Ronald
R
Dehoux
Mischaël
M
Thissen
Jean Paul
JP
Francaux
Marc
M
D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003401:Creatine / Q000494:pharmacology*; D017931:DNA Primers; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics*; D007334:Insulin-Like Growth Factor I / Q000187:drug effects / Q000235:genetics*; D007700:Kinetics; D051379:Mice; D032446:Myoblasts; D018006:Myogenic Regulatory Factors / Q000235:genetics*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D018006:Myogenic Regulatory Factors; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D003401:Creatine