title: Influence of a maternal cholesterol-enriched diet on [1-14C]-linoleic acid and L-[4, 5-3H]-leucine entry in plasma of rabbit offspring.
abstract: Fetal development requires an important entry of essential free fatty acids (EFFA) and essential amino acids (EAA) into the fetal circulation. We have reported that a 0.2% enriched-cholesterol diet (ECD) during rabbit gestation significantly reduces fetus weight compared to control diet. It is known that dietary linoleic acid deficiency, an EFFA, during the fetal development induces an important impair to the somatic development. Moreover, intrauterine growth retardation induced a reduction of the flux of leucine, an EAA, from maternal to fetal circulation. Therefore, we hypothesized that the administration of an ECD induces modifications of placental lipid composition concomitant alterations of the transfer of linoleic acid and leucine in fetal circulation. Quantification of placental lipids revealed that in the ECD group a reduction of total-cholesterol (TC) and free-cholesterol (FC) is observed, however an increased in FFA and phospholipids is noticed when compared to the control group. In placenta from the ECD group, the FC/ TC ratio is significantly reduced compared to the control group. In the ECD group, the liver shows an increase of TC, FC and FFA compared to the control group. However, the quantity of triacylglycerol present in the liver from the ECD is significantly reduced compared to the control group. To evaluate the placental transfer of some essential nutrients, intravenous injection of [1-14C]-linoleic acid or L-[4, 5-3H]-leucine to term rabbit (control and ECD group) were done. Two hours later, rabbits were euthanized and we collected placenta, livers and blood from dams and offspring. The concentrations of both radiolabeled molecules (linoleic acid and its esterified form or leucine) were higher in the plasma of ECD offspring than those found in offspring from control diet. Despite such alteration of placental lipid composition, linoleic acid and leucine transfer by the placenta was not compromised but rather increased.
authors.affiliation: Laboratoire de Physiologie Materno-Foetale, Université du Québec à Montréal, Montréal, Canada, H3C 3P8.
authors.forename: Alain
authors.initials: A
authors.lastname: Montoudis
authors.forename: Lucie
authors.initials: L
authors.lastname: Simoneau
authors.forename: Julie
authors.initials: J
authors.lastname: Lafond
mesh_terms: D000818:Animals; D002250:Carbon Radioisotopes; D002791:Cholesterol, Dietary / Q000008:administration & dosage*; D005260:Female; D005312:Fetal Blood / Q000737:chemistry; D005333:Fetus / Q000187:drug effects* / Q000378:metabolism; D006937:Hypercholesterolemia / Q000378:metabolism*; D007275:Injections, Intravenous; D007930:Leucine / Q000008:administration & dosage / Q000493:pharmacokinetics*; D019787:Linoleic Acid / Q000008:administration & dosage / Q000493:pharmacokinetics*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008431:Maternal-Fetal Exchange / Q000187:drug effects*; D010920:Placenta / Q000187:drug effects / Q000378:metabolism; D011247:Pregnancy; D011817:Rabbits; D014316:Tritium
chemical_list: D002250:Carbon Radioisotopes; D002791:Cholesterol, Dietary; D014316:Tritium; D019787:Linoleic Acid; D007930:Leucine