title: Role of the PLA2-independent peroxiredoxin VI activity in the survival of immortalized fibroblasts exposed to cytotoxic oxidative stress.
abstract: Peroxiredoxin VI (PrxVI) is a bifunctional enzyme with non-selenium glutathione peroxidase and Ca2+-independent acidic phospholipase A2 activities. We demonstrate that transfection-mediated PrxVI overexpression protects immortalized human WI-38 and murine NIH3T3 fibroblasts against cytotoxic doses of tert-butylhydroperoxide and H2O2. Mutants for either glutathione peroxidase or phospholipase A2 activity show that glutathione peroxidase but not phospholipase A2 activity is required to promote cell survival after stress. Also, ectopic PrxVI overexpression does not protect telomerase-stabilized WI-38 fibroblasts against stress-induced premature senescence.
authors.affiliation: Research Unit of Cellular Biology, University of Namur (FUNDP), rue de Bruxelles 61, B-5000 Namur, Belgium.
authors.forename: Michel
authors.initials: M
authors.lastname: Salmon
authors.forename: Jérémie
authors.initials: J
authors.lastname: Dedessus Le Moutier
authors.forename: Frédéric
authors.initials: F
authors.lastname: Wenders
authors.forename: Sophie
authors.initials: S
authors.lastname: Chiarizia
authors.forename: François
authors.initials: F
authors.lastname: Eliaers
authors.forename: José
authors.initials: J
authors.lastname: Remacle
authors.forename: Véronique
authors.initials: V
authors.lastname: Royer
authors.forename: Thierry
authors.initials: T
authors.lastname: Pascal
authors.forename: Olivier
authors.initials: O
authors.lastname: Toussaint
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D016922:Cellular Senescence / Q000187:drug effects / Q000502:physiology*; D005347:Fibroblasts / Q000166:cytology* / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D051379:Mice; D018384:Oxidative Stress / Q000502:physiology*; D010544:Peroxidases / Q000235:genetics* / Q000378:metabolism; D054465:Peroxiredoxin VI; D054464:Peroxiredoxins; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection; D020122:tert-Butylhydroperoxide / Q000633:toxicity*
chemical_list: D011994:Recombinant Proteins; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D010544:Peroxidases; D054465:Peroxiredoxin VI; D054464:Peroxiredoxins; D010741:Phospholipases A; D054467:Phospholipases A2