Modulation of KCNQ4 channel activity by changes in cell volume.
KCNQ4 channels expressed in HEK 293 cells are sensitive to cell volume changes, being activated by swelling and inhibited by shrinkage, respectively. The KCNQ4 channels contribute significantly to the regulatory volume decrease (RVD) process following cell swelling. Under isoosmotic conditions, the KCNQ4 channel activity is modulated by protein kinases A and C, G protein activation, and a reduction in the intracellular Ca2+ concentration, but these signalling pathways are not responsible for the increased channel activity during cell swelling.
Hougaard
Charlotte
C
Biochemical Department, The August Krogh Institute, University of Copenhagen, Universitetsparken 13, DK-2100 Copenhagen, Denmark.
Klaerke
Dan A
DA
Hoffmann
Else K
EK
Olesen
Søren-Peter
SP
Jorgensen
Nanna K
NK
D002118:Calcium / Q000494:pharmacology; D002219:Carbamates / Q000494:pharmacology; D002460:Cell Line; D048429:Cell Size / Q000187:drug effects / Q000502:physiology*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000494:pharmacology; D006801:Humans; D007211:Indoles; D051656:KCNQ Potassium Channels; D051658:KCNQ2 Potassium Channel; D051659:KCNQ3 Potassium Channel; D009994:Osmolar Concentration; D018408:Patch-Clamp Techniques; D010655:Phenylenediamines / Q000494:pharmacology; D026902:Potassium Channel Blockers / Q000494:pharmacology; D015221:Potassium Channels / Q000096:biosynthesis / Q000187:drug effects / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated; D011493:Protein Kinase C / Q000494:pharmacology; D011725:Pyridines; D015398:Signal Transduction / Q000187:drug effects
D002219:Carbamates; D007211:Indoles; D051656:KCNQ Potassium Channels; D051658:KCNQ2 Potassium Channel; C496914:KCNQ2 protein, human; D051659:KCNQ3 Potassium Channel; C496916:KCNQ3 protein, human; C117367:KCNQ4 protein, human; D010655:Phenylenediamines; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D011725:Pyridines; C101866:ezogabine; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; C061017:linopirdine; D002118:Calcium