title: High levels of soluble herpes virus entry mediator in sera of patients with allergic and autoimmune diseases.
abstract: Herpes virus entry mediator (HVEM) is a newly discovered member of the tumor necrosis factor receptor (TNFR) superfamily that has a role in herpes simplex virus entry, in T cell activation and in tumor immunity. We generated mAb against HVEM and detected soluble HVEM (SHVEM) in the sera of patients with various autoimmune diseases. HVEM was constitutively expressed on CD4(+) and CD8(+) T cells, CD19(+) B cells, CD14(+) monocytes, neutrophils and dendritic cells. In three-way MLR, mAb 122 and 139 were agonists and mAb 108 had blocking activity. An ELISA was developed to detect sHVEM in patient sera. sHVEM levels were elevated in sera of patients with allergic asthma, atopic dermatitis and rheumatoid arthritis. The mAbs discussed here may be useful for studies of the role of HVEM in immune responses. Detection of soluble HVEM might have diagnostic and prognostic value in certain immunological disorders.
authors.affiliation: The Immunomodulation Research Center and Department of Biological Sciences, University of Ulsan, Ulsan 680-749, Korea.
authors.forename: Hyo Won
authors.initials: HW
authors.lastname: Jung
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: La
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Suk Kyeung
authors.initials: SK
authors.lastname: Heo
authors.forename: Ju Yang
authors.initials: JY
authors.lastname: Kim
authors.forename: Sa
authors.initials: S
authors.lastname: Wang
authors.forename: Kack Kyun
authors.initials: KK
authors.lastname: Kim
authors.forename: Ki Man
authors.initials: KM
authors.lastname: Lee
authors.forename: Hong Rae
authors.initials: HR
authors.lastname: Cho
authors.forename: Hyeon Woo
authors.initials: HW
authors.lastname: Lee
authors.forename: Byungsuk
authors.initials: B
authors.lastname: Kwon
authors.forename: Byung Sam
authors.initials: BS
authors.lastname: Kim
authors.forename: Byoung Se
authors.initials: BS
authors.lastname: Kwon
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology; D001249:Asthma / Q000097:blood / Q000276:immunology; D001327:Autoimmune Diseases / Q000097:blood* / Q000276:immunology; D002455:Cell Division; D002460:Cell Line; D003876:Dermatitis, Atopic / Q000097:blood / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006967:Hypersensitivity / Q000097:blood* / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D018124:Receptors, Tumor Necrosis Factor / Q000097:blood* / Q000276:immunology; D053240:Receptors, Tumor Necrosis Factor, Member 14; D011991:Receptors, Virus / Q000097:blood* / Q000276:immunology; D012995:Solubility
chemical_list: D000911:Antibodies, Monoclonal; D018124:Receptors, Tumor Necrosis Factor; D053240:Receptors, Tumor Necrosis Factor, Member 14; D011991:Receptors, Virus; C506028:TNFRSF14 protein, human; C506029:Tnfrsf14 protein, mouse