title: Chondrogenic potential of multipotential cells from human adipose tissue.
abstract: The use of stem cells for cell-based tissue-engineering strategies represents a promising alternative for the repair of cartilaginous defects. The multilineage potential of a population of putative mesodermal stem cells obtained from human lipoaspirates, termed processed lipoaspirate cells, was previously characterized. The chondrogenic potential of those cells was confirmed with a combination of histological and molecular approaches. Processed lipoaspirate cells under high-density micromass culture conditions, supplemented with transforming growth factor-beta1, insulin, transferrin, and ascorbic acid, formed well-defined nodules within 48 hours of induction and expressed the cartilaginous markers collagen type II, chondroitin-4-sulfate, and keratan sulfate. Reverse transcription polymerase chain reaction analysis confirmed the expression of collagen type II and the cartilage-specific proteoglycan aggrecan. In summary, human adipose tissue may represent a novel plentiful source of multipotential stem cells capable of undergoing chondrogenesis in vitro.
authors.affiliation: Department of Surgery, University of California, Los Angeles School of Medicine, 90095-6902, USA.
authors.forename: Jerry I
authors.initials: JI
authors.lastname: Huang
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Zuk
authors.forename: Neil F
authors.initials: NF
authors.lastname: Jones
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.forename: H Peter
authors.initials: HP
authors.lastname: Lorenz
authors.forename: Marc H
authors.initials: MH
authors.lastname: Hedrick
authors.forename: Prosper
authors.initials: P
authors.lastname: Benhaim
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D053674:Aggrecans; D002356:Cartilage / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D020219:Chondrogenesis; D002809:Chondroitin Sulfates / Q000378:metabolism; D024043:Collagen Type II / Q000378:metabolism; D004396:Coloring Agents; D006801:Humans; D007632:Keratan Sulfate / Q000378:metabolism; D008875:Middle Aged; D011509:Proteoglycans / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D053674:Aggrecans; D024043:Collagen Type II; D004396:Coloring Agents; D011509:Proteoglycans; D002809:Chondroitin Sulfates; D007632:Keratan Sulfate