Thioglycosynthases: double mutant glycosidases that serve as scaffolds for thioglycoside synthesis.
A double mutant, retaining glycosidase that lacks both the catalytic nucleophile and the catalytic acid/base residues efficiently catalyzes thioglycoside formation from a glycosyl fluoride donor and thiosugar acceptors.
Jahn
Michael
M
Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, B.C. V6T 1Z1, Canada.
Chen
Hongming
H
Müllegger
Johannes
J
Marles
Jennifer
J
Warren
R Antony J
RA
Withers
Stephen G
SG
D002942:Circular Dichroism; D004795:Enzyme Stability; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D009154:Mutation / Q000235:genetics*; D013696:Temperature; D013865:Thioglycosides / Q000096:biosynthesis* / Q000737:chemistry
D013865:Thioglycosides; D006026:Glycoside Hydrolases