State of chromatin sensitivity to DNase I in the rat Ig-beta/growth hormone locus determined by real-time PCR.
Using Ig-beta and growth hormone producing cells with liver-derived cells for controls, sensitivity of chromatin to DNase I was measured by real-time PCR at eleven targets in rat Ig-beta/growth hormone locus where four cell type-specific genes and two ubiquitously expressed genes are present in a compact 88-kb region. Chromatin situated at the promoter of actively-transcribed gene and placed at cell type-specific DNase I hypersensitive sites with enhancer activity was sensitive to DNase I. In the case of inactive gene, chromatin located in these regions was resistant to DNase I. Unexpectedly, however, chromatin placed in the transcribed intron was resistant to DNase I in two genes. DNase I sensitive chromatin was shown not to distribute locus-widely but rather to localize at the promoter and the enhancer of actively-transcribed genes in this locus.
Osano
Kyoichi
K
Department of Life Science, and Frontier Project Life's Adaptation Strategies to Environmental Changes, College of Science, Rikkyo University, Toshima-ku, Tokyo 171-8501, Japan.
Otsuka
Akira
A
Ono
Masao
M
D000818:Animals; D002478:Cells, Cultured; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D005091:Exons; D013006:Growth Hormone / Q000235:genetics / Q000378:metabolism*; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism*; D007438:Introns; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D051381:Rats
D002843:Chromatin; D007136:Immunoglobulins; C038767:immunoglobulin B; D013006:Growth Hormone; D003850:Deoxyribonuclease I