title: Improved display of synthetic IgG-binding domains on the baculovirus surface.
abstract: Improved display of foreign protein moieties in combination with beneficial alteration of the viral surface properties should be of value for targeted and enhanced gene delivery. Here, we describe a vector based on Autographa californica multiple nucleopolyhedrovirus (AcMNPV) displaying synthetic IgG-binding domains (ZZ) of protein A fused to the transmembrane anchor of vesicular stomatitis virus (VSV) G protein. This display vector was equipped with a GFP/EGFP expression cassette enabling fluorescent detection in both insect and mammalian cells. The virus construct displayed the biologically active fusion protein efficiently and showed increased binding capacity to IgG. As the display is carried out using a membrane anchor of foreign origin, gp64 is left intact for virus entry, which may increase gene expression in the transduced mammalian cells. In addition, the viral vector can be targeted to any desired cell type via binding of ZZ domains when an appropriate IgG antibody is available.
authors.affiliation: University of Jyvaskyla, Department of Biological and Environmental Science, University of Jyvaskyla, POB 35, FIN-40014, Finland.
authors.forename: Kirsi
authors.initials: K
authors.lastname: Ojala
authors.forename: Johanna
authors.initials: J
authors.lastname: Koski
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Ernst
authors.forename: Reingard
authors.initials: R
authors.lastname: Grabherr
authors.forename: Ian
authors.initials: I
authors.lastname: Jones
authors.forename: Christian
authors.initials: C
authors.lastname: Oker-Blom
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000648:ultrastructure; D001666:Binding Sites, Antibody / Q000235:genetics* / Q000276:immunology*; D002460:Cell Line; D006224:Cricetinae; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics; D017924:Nucleopolyhedroviruses / Q000235:genetics; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000648:ultrastructure; D018411:Spodoptera; D014721:Vesicular stomatitis Indiana virus; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: C028391:G protein, vesicular stomatitis virus; D007106:Immune Sera; D007074:Immunoglobulin G; D008562:Membrane Glycoproteins; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins