The Yersinia adhesin YadA collagen-binding domain structure is a novel left-handed parallel beta-roll.
The crystal structure of the recombinant collagen-binding domain of Yersinia adhesin YadA from Yersinia enterocolitica serotype O:3 was solved at 1.55 A resolution. The trimeric structure is composed of head and neck regions, and the collagen binding head region is a novel nine-coiled left-handed parallel beta-roll. Before the beta-roll, the polypeptide loops from one monomer to the rest, and after the beta-roll the neck region does the same, making the transition from the globular head region to the narrower stalk domain. This creates an intrinsically stable 'lock nut' structure. The trimeric form of YadA is required for collagen binding, and mutagenesis of its surface residues allowed identification of a putative collagen-binding surface. Furthermore, a new structure-sequence motif for YadA beta-roll was used to identify putative YadA-head-like domains in a variety of human and plant pathogens. Such domains may therefore be a common bacterial strategy for avoiding host response.
Nummelin
Heli
H
Macromolecular X-ray Crystallography Group, Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
Merckel
Michael C
MC
Leo
Jack C
JC
Lankinen
Hilkka
H
Skurnik
Mikael
M
Goldman
Adrian
A
D018829:Adhesins, Bacterial / Q000737:chemistry*; D000595:Amino Acid Sequence; D003094:Collagen / Q000737:chemistry*; D003460:Crystallization; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D015008:Yersinia enterocolitica / Q000737:chemistry* / Q000235:genetics
D018829:Adhesins, Bacterial; D011994:Recombinant Proteins; C064832:YadA protein, Yersinia; D003094:Collagen