title: Chalcone dimethylallyltransferase from Morus nigra cell cultures. Substrate specificity studies.
abstract: A new prenyltransferase (PT) enzyme derived from the microsomal fractions of cell cultures of Morus nigra was shown to be able to prenylate exclusively chalcones with a 2',4'-dihydroxy substitution and the isoflavone genistein. Computational studies were performed to shed some light on the relationship between the structure of the substrate and the enzymatic activity. PT requires divalent cations, particularly Mg(2+), to be effective. The apparent K(m) values for gamma,gamma-dimethylallyldiphosphate and 2',4'-dihydroxychalcone were 63 and 142 microM, respectively. The maximum activity of the enzyme was expressed during the first 10 days of cell growth.
authors.affiliation: Istituto di Chimica del Riconoscimento Molecolare, Sezione di Roma, Istituto di Biochimica e Biochimica Clinica, Università Cattolica del S. Cuore, Largo F. Vito 1, 00168 Rome, Italy. a.vitali@uniserve.ccr.rm.cnr.it
authors.forename: Alberto
authors.initials: A
authors.lastname: Vitali
authors.forename: Bruno
authors.initials: B
authors.lastname: Giardina
authors.forename: Giuliano
authors.initials: G
authors.lastname: Delle Monache
authors.forename: Filippo
authors.initials: F
authors.lastname: Rocca
authors.forename: Andrea
authors.initials: A
authors.lastname: Silvestrini
authors.forename: Andrea
authors.initials: A
authors.lastname: Tafi
authors.forename: Bruno
authors.initials: B
authors.lastname: Botta
mesh_terms: D002478:Cells, Cultured; D002599:Chalcone / Q000031:analogs & derivatives / Q000737:chemistry* / Q000378:metabolism*; D004122:Dimethylallyltranstransferase / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008861:Microsomes / Q000201:enzymology; D008968:Molecular Conformation; D031625:Morus / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D002599:Chalcone; D004122:Dimethylallyltranstransferase