AmpliBASE MT: a Mycobacterium tuberculosis diversity knowledgebase.
AmpliBASE MT is an online databank of high-resolution DNA fingerprints representing fluorescent amplified fragment length polymorphism (FAFLP) profiles or amplitypes developed for the Mycobacterium tuberculosis complex strains from 48 different countries. AmpliBASE MT is based on a relational database management system that is hyperlinked to visualize genotyping results in the form of DNA fingerprint images for individual strains. A flexible search system based on systematic comparisons of fragment sizes in base pairs allows inter-laboratory comparison of FAFLP profiles. Besides this, the database also displays previously published data on IS6110 profiles, spoligotypes, MIRU-VNTRs and large sequence polymorphisms along with the FAFLP records that will give the overall comparisons. Being the first of its kind, AmpliBASE MT is expected to be a very helpful tool in strengthening the concept of 'geographic genomics' and will be very helpful to molecular epidemiologists and those interested in diagnostic development for tuberculosis.
Majeed
Ahmed A
AA
Centre for DNA Fingerprinting and Diagnostics, Nacharam, Hyderabad 500076, India.
Ahmed
Niyaz
N
Rao
K Rajender
KR
Ghousunnissa
S
S
Kauser
Farhana
F
Bose
Buddhaditta
B
Nagarajaram
H A
HA
Katoch
V M
VM
Cousins
Debby V
DV
Sechi
Leonardo A
LA
Gilman
Robert H
RH
Hasnain
Seyed E
SE
D016172:DNA Fingerprinting / Q000379:methods*; D003628:Database Management Systems; D030561:Databases, Nucleic Acid; D020869:Gene Expression Profiling / Q000379:methods*; D014644:Genetic Variation / Q000235:genetics; D016247:Information Storage and Retrieval / Q000379:methods*; D020407:Internet; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D010641:Phenotype; D012984:Software; D014584:User-Computer Interface