[Epitope of the catalytic subunit of viscumin, exposed on its interaction with a lipid bilayer].
It was previously shown that the catalytic subunit of the plant toxin viscumin induces aggregation of small unilamellar liposomes and this process is inhibited by the mab_TA7 monoclonal antibody produced to the denatured catalytic subunit of viscumin (Agapov, I.I. et al., FEBS Lett., 1999, vol. 464, pp. 63-66). The interaction of the synthetic F101-T105 and A96-T105 fragments of the viscumin catalytic subunit with the mab_TA7 monoclonal antibody was studied by 1H NMR spectroscopy. The results of this study demonstrated that only the A96-T105 fragment is capable of binding to mab_TA7. A nuclear Overhauser effect observed in the antigen-antibody complex and registered on the resonances of the free peptide and exchanging between the free state and the antibody-bound state was analyzed; the mab_TA7 antigen determinant (H99-T105) was identified; and its conformation and orientation within the complex with the antibody were determined. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2003, vol. 29, no. 6; see also http://www.maik.ru.
Pashkov
V S
VS
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP Moscow, 117997 Russia. pavs@nmr.ru
Agapov
I I
II
Balashova
T A
TA
Egorova
N S
NS
Surovoĭ
A Iu
AIu
Pevzner
I B
IB
Tonevitskiĭ
A G
AG
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000737:chemistry; D002384:Catalysis; D000939:Epitopes / Q000737:chemistry*; D008051:Lipid Bilayers; D019906:Nuclear Magnetic Resonance, Biomolecular; D028321:Plant Preparations / Q000737:chemistry*; D010940:Plant Proteins; D011489:Protein Denaturation; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D008051:Lipid Bilayers; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C009674:ribosome inactivating protein, Viscum