Analysis of catecholamines and related substances using porous graphitic carbon as separation media in liquid chromatography-tandem mass spectrometry.
Capillary porous graphitic carbon (PGC) columns have been utilized for separation of several catecholamines and related compounds (i.e. L-tyrosine, L-DOPA, 3-O-methyl-DOPA, dopamine, 3,4-dihydroxy-phenyl-acetic acid (DOPAC), homovanillic acid, noradrenaline, vanillomandelic acid and adrenaline) on-line with electrospray ionization tandem mass spectrometry (ESI-MS/MS). The use of a mobile phase without ion-pairing agents and with high content of organic modifier facilitated the coupling to the selective and sensitive mass spectrometric detection. Minimum detectable sample concentration (MDC sample) for noradrenaline, dopamine and L-tyrosine in a standard solution was estimated to 3, 10 and 30 nM, respectively (3 S/N corresponds to MDQ for L-tyrosine of approximately 8 x 10(-14)mol). The developed strategy was applied for analysis of brain tissue, i.e. a substantia nigra (ns) sample.
Törnkvist
Anna
A
Department of Analytical Chemistry, Institute of Chemistry, Uppsala University, P.O. Box 599, SE 75124 Uppsala, Sweden.
Sjöberg
Per J R
PJ
Markides
Karin E
KE
Bergquist
Jonas
J
D015102:3,4-Dihydroxyphenylacetic Acid / Q000302:isolation & purification; D000818:Animals; D001923:Brain Chemistry; D002395:Catecholamines / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods*; D004298:Dopamine / Q000302:isolation & purification; D004837:Epinephrine / Q000302:isolation & purification; D006108:Graphite; D006863:Hydrogen-Ion Concentration; D007980:Levodopa / Q000302:isolation & purification; D009638:Norepinephrine / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013378:Substantia Nigra / Q000737:chemistry; D013552:Swine; D014443:Tyrosine / Q000302:isolation & purification; D014642:Vanilmandelic Acid / Q000302:isolation & purification
D002395:Catecholamines; D015102:3,4-Dihydroxyphenylacetic Acid; D014443:Tyrosine; D007980:Levodopa; D014642:Vanilmandelic Acid; D006108:Graphite; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine