title: Capillary isoelectric focusing in pseudo-closed channel coupled to matrix assisted laser desorption/ionization mass spectrometry for protein analysis.
abstract: Capillary isoelectric focusing (CIEF) was performed in pseudo-closed channel to separate proteins on a plastic chip. Pseudo-closed channel provided a novel way to couple protein separation by CIEF to MALDI mass spectrometry without eluting the focused proteins.
authors.affiliation: Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672, Singapore. szen@gis.a-star.edu.sg
authors.forename: Michelle L-S
authors.initials: ML
authors.lastname: Mok
authors.forename: Lin
authors.initials: L
authors.lastname: Hua
authors.forename: Jonathan B-C
authors.initials: JB
authors.lastname: Phua
authors.forename: Michael K-T
authors.initials: MK
authors.lastname: Wee
authors.forename: Newman S-K
authors.initials: NS
authors.lastname: Sze
mesh_terms: D000818:Animals; D002417:Cattle; D006736:Horses; D007525:Isoelectric Focusing / Q000379:methods; D009211:Myoglobin / Q000032:analysis; D040081:Protein Array Analysis / Q000379:methods; D011506:Proteins / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D009211:Myoglobin; D011506:Proteins