'Agglutination and flocculation' of stem cells collected by apheresis due to cryofibrinogen.
Collection of peripheral stem cells by apheresis is a well-described process. Here, investigations concerning 'agglutination and flocculation' of stem cells collected from two patients are described. In both cases, cryoproteins were observed and cryofibrinogen was identified using high-resolution two-dimensional electrophoresis. In one case, peripheral stem cells were collected after a second course of mobilization, and the cells were immediately washed at 37 degrees C before being frozen, allowing their use, despite the presence of cryofibrinogen. In the other case, 'agglutination' was reversed by warming the bag, and plasma was removed before freezing.
Siegenthaler
M A
MA
Service Régional Vaudois de Transfusion Sanguine, Lausanne, Switzerland.
Vu
D-H
DH
Ebnöther
M
M
Ketterer
N
N
Luthi
F
F
Schmid
P
P
Bargetzi
M
M
Gasparini
D
D
Tissot
J-D
JD
D000371:Agglutination / Q000187:drug effects; D001781:Blood Component Removal; D002448:Cell Adhesion / Q000187:drug effects; D003450:Cryoglobulins / Q000302:isolation & purification / Q000494:pharmacology*; D015925:Cryopreservation; D015180:Electrophoresis, Gel, Two-Dimensional; D015241:Fibrinogens, Abnormal / Q000302:isolation & purification / Q000494:pharmacology*; D005427:Flocculation; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D014182:Transplantation, Autologous
D003450:Cryoglobulins; D015241:Fibrinogens, Abnormal; C041874:cryofibrinogen