Variability of the progeny of a sequence variant Citrus bent leaf viroid (CBLVd).
A field isolate of CBLVd was previously shown to contain two dominant subpopulations (I and II), which differed by the presence or absence of a Sal I restriction site in the PCR product [10]. Here we demonstrate the infectivity and symptom expression of subpopulation II by inoculating Etrog citron with a single representative haplotype. The resulting progeny was characterised as an heterogeneous population of closely related variants with a new fitness peak represented by an haplotype that was not identified in the original isolate. This demonstrates that CBLVd conforms a "quasispecies" model. The progeny shared features of the two subpopulations of the original isolate indicating that the original isolate probably arose from a single CBLVd ancestor.
Gandía
M
M
Instituto Valenciano de Investigaciones Agrarias, Moncada, Valencia, Spain.
Duran-Vila
N
N
D001483:Base Sequence; D002957:Citrus / Q000821:virology*; D019295:Computational Biology; D014644:Genetic Variation / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D010942:Plant Viruses / Q000235:genetics; D018807:Polymorphism, Single-Stranded Conformational; D012367:RNA, Viral / Q000235:genetics; D012984:Software; D014772:Viroids / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication
D012367:RNA, Viral