Determination of ochratoxin A in wine using liquid-phase microextraction combined with liquid chromatography with fluorescence detection.
A liquid-liquid microextraction technique (LPME) has been applied to the extraction of ochratoxin A (OTA) from wine prior to its quantification by HPLC-fluorescence detection. OTA was extracted from wine, through 1-octanol immobilized in the pores of a porous hollow fiber, and introduced into 1-octanol inside the fiber. Recovery was 77%. The method was adequate for quantification of OTA in wine at levels within the range 0.25-10 ng/ml with a LOD of 0.2 ng/ml, and can be a simple and inexpensive alternative to the use of inmunoaffinity columns in order to quantify OTA levels in wine.
González-Peñas
E
E
Organic and Pharmaceutical Chemistry Department, Faculty of Pharmacy, University of Navarra, C/Irunlarrea s/n, 31008 Pamplona, Spain. mgpenas@unav.es
Leache
C
C
Viscarret
M
M
Pérez de Obanos
A
A
Araguás
C
C
López de Cerain
A
A
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009793:Ochratoxins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014920:Wine / Q000032:analysis*
D009793:Ochratoxins; C025589:ochratoxin A