Nuclear and mitochondrial DNA microsatellite instability in hepatocellular carcinoma in Chinese.
OBJECTIVE
To study the nuclear microsatellite instability (nMSI) at BAT26 and mitochondrial microsatellite instability (mtMSI) in the occurrence and development of hepatocellular carcinoma and the relationship between nMSI and mtMSI.


METHODS
nMSI was observed with PCR and mtMSI with PCR-SSCP in 52 cases of hepatocellular carcinoma.


RESULTS
mtMSI was detected in 11 out of the 52 cases of hepatocellular carcinoma (21.2%). Among the 11 cases of hepatocellular carcinoma with mtMSI, 7 occurred in one locus and 4 in 2 loci. The frequency of mtMSI in the 52 cases of hepatocellular carcinoma showed no correlation to sex, age, infection of hepatitis B, liver cirrhosis as well as positive AFP of the patients (P>0.05). In addition, nMSI was detected in 3 out of 52 cases of hepatocellular carcinoma (5.8%) and there was no correlation of the incidence of mtMSI to that of nMSI (P>0.05).


CONCLUSIONS
mtMSI may be involved in the coccurrence and development of hepatocellular carcinoma and it is independent of nMSI.
Fang
Dian-Chun
DC
Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China. fangdianchun@hotmail.com
Fang
Li
L
Wang
Rong-Quan
RQ
Yang
Shi-Ming
SM
D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002467:Cell Nucleus; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged
D004272:DNA, Mitochondrial