title: Identification of peptide sequences that induce the transport of phage across the gastrointestinal mucosal barrier.
abstract: To investigate whether specific peptide sequences could induce virion transport across the intestinal barrier, we used phage display to both identify signalling peptides capable of inducing trans-intestinal transport and also provide a suitable model of virion translocation. We utilised simple, single-round high input in vivo biopanning protocol using a 7-mer random amino acid phage display library. Phage were applied by gavage and translocation across the intestinal barrier assessed by phage recovery from the spleen 2h later. Following isolation, a number of phage were sequenced and several homologies with HIV gp120 were identified. Immunocytochemical analysis of phage translocation across the intestinal barrier by a phage bearing the peptide YPRLLTP demonstrated that phage were actively transported along specific channels. It is concluded that utilisation of in vivo phage display (IVPD) has provided evidence for a specific peptide-guided transport of undegraded cargo across the intestinal barrier, modelled by M13 phage.
authors.affiliation: Institute of Brain Research, University of Tuebingen, Calwer Str. 3, D-72076 Tuebingen, Germany.
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Duerr
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: White
authors.forename: Hermann J
authors.initials: HJ
authors.lastname: Schluesener
mesh_terms: D000818:Animals; D017104:Bacteriophage M13 / Q000302:isolation & purification / Q000502:physiology*; D005753:Gastric Mucosa / Q000821:virology*; D005772:Gastrointestinal Transit; D007413:Intestinal Mucosa / Q000821:virology*; D051381:Rats; D011917:Rats, Inbred Lew; D014773:Virology / Q000379:methods; D018406:Virus Physiological Phenomena