[Effects of acrylamide on DNA damage in human keratinocytes].
OBJECTIVE
To investigate the toxic and DNA damaging effect of acrylamide (AA) on human keratinocytes and its mechanism.


METHODS
(1) After the keratinocyte cell line HaCaT cells were exposed to AA with different concentrations for 44 hours, cell survival rate was detected by MTT method. (2) The effects of DNA damage of exposed cells were detected by comet assay. (3) After treating the cells with 2.00 mmol/L of AA plus 0.50 mmol/L of 1-aminobenzotriazole (1-ABT), an inhibitor of cytochrome P-450 enzymes (CYP-450), for 4 hours, the relationship between DNA damage and CYP-450 was studied.


RESULTS
(1) Cytotoxicity measurement of AA showed that cell survival rate decreased significantly after 44-hour treatment. (2) Cytotoxicity was not detected after 4-hour AA treatment, but significant DNA damage was observed in all treatment groups, and the degree of damage increased with the concentration of AA. Moreover, the tail lengths of comet cells were in dose-effect relationship. As for cells treated by 1-ABT with 2 mmol/L AA, comet rate and tail length were 15.4% and (8.2 +/- 2.0) micro m respectively, which were decreased significantly (P < 0.01) when compared with 2 mmol/L AA treatment group [80.6% and (44.3 +/- 4.0) micro m].


CONCLUSIONS
Acrylamide has significant cytotoxicity and genotoxicity on HaCaT cells. AA-induced DNA damage may be related to the oxidative metabolite(s) of AA through CYP-450.
Ma
Xiao-xia
XX
Institute of Occupational and Environmental Health, Zhejiang University, Hangzhou 310031, China.
Yao
Geng-dong
GD
Cheng
Hao
H
Zeng
Qun-li
QL
Chen
Qing
Q
D020106:Acrylamide / Q000633:toxicity*; D002478:Cells, Cultured; D065607:Cytochrome P-450 Enzyme Inhibitors; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000201:enzymology
D065607:Cytochrome P-450 Enzyme Inhibitors; D020106:Acrylamide