title: [Preparation of DNA-duplexes, containing internucleotide thiophosphate groups in various positions of the recognition site for the EcoRII restriction endonuclease].
abstract: Twenty-four 12-mer DNA duplexes, each containing a chiral phosphorothioate group successively replacing one of the internucleotide phosphate groups either in the EcoRII recognition site (5'CCA/TGG) or near to it, were obtained for studying the interaction of the restriction endonuclease EcoRII with internucleotide DNA phosphates. Twelve of the 12-mer oligonucleotides were synthesized as Rp and Sp diastereomeric mixtures. Six of them were separated by reversed-phase HPLC using various buffers. Homogeneous diastereomers of the other oligonucleotides were obtained by enzymatic ligation of the Rp and Sp diastereomers of 5- to 7-mer oligonucleotides preliminarily separated by HPLC with the corresponding short oligonucleotides on a complementary DNA template. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2003, vol. 29, no. 6; see also http://www.maik.ru.
authors.affiliation: Department of Chemistry, Belozersky Institute of Physicochemical Biology, Moscow State University, Vorob'evy gory, Moscow, 119992 Russia.
authors.forename: F B
authors.initials: FB
authors.lastname: Subach
authors.forename: S
authors.initials: S
authors.lastname: Miuller
authors.forename: V N
authors.initials: VN
authors.lastname: Tashlitskiĭ
authors.forename: O V
authors.initials: OV
authors.lastname: PIatrauskene
authors.forename: E S
authors.initials: ES
authors.lastname: Gromova
mesh_terms: D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism*
chemical_list: D009943:Organophosphorus Compounds; D004247:DNA; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific