title: Phase 1 studies of Walter Reed Army Institute of Research candidate attenuated dengue vaccines: selection of safe and immunogenic monovalent vaccines.
abstract: We describe the results of initial safety testing of 10 live-attenuated dengue virus (DENV) vaccine candidates modified by serial passage in primary dog kidney (PDK) cells at the Walter Reed Army Institute of Research. The Phase 1 studies, conducted in 65 volunteers, were designed to select an attenuated vaccine candidate for each DENV serotype. No recipient of the DENV candidate vaccines sustained serious injury or required treatment. Three vaccine candidates were associated with transient idiosyncratic reactions in one volunteer each, resulting in their withdrawal from further clinical development. Increasing PDK cell passage of DENV-1, DENV-2, and DENV-3 candidate vaccines increased attenuation for volunteers, yet also decreased infectivity and immunogenicity. This effect was less clear for DENV-4 candidate vaccines following 15 and 20 PDK cell passages. Only one passage level each of the tested DENV-2, -3, and -4 vaccine candidates was judged acceptably reactogenic and suitable for expanded clinical study. Subsequent studies with more recipients will further establish safety and immunogenicity of the four selected vaccine candidates: DENV-1 45AZ5 PDK 20, DENV-2 S16803 PDK 50, DENV-3 CH53489 PDK 20, and DENV-4 341750 PDK 20.
authors.affiliation: Walter Reed Army Institute of Research, Washington, District of Columbia, USA. niranjan.kanesa-thasan@det.amedd.army.mil
authors.forename: N
authors.initials: N
authors.lastname: Kanesa-Thasan
authors.forename: R
authors.initials: R
authors.lastname: Edelman
authors.forename: C O
authors.initials: CO
authors.lastname: Tacket
authors.forename: S S
authors.initials: SS
authors.lastname: Wasserman
authors.forename: D W
authors.initials: DW
authors.lastname: Vaughn
authors.forename: T S
authors.initials: TS
authors.lastname: Coster
authors.forename: G J
authors.initials: GJ
authors.lastname: Kim-Ahn
authors.forename: D R
authors.initials: DR
authors.lastname: Dubois
authors.forename: J R
authors.initials: JR
authors.lastname: Putnak
authors.forename: A
authors.initials: A
authors.lastname: King
authors.forename: P L
authors.initials: PL
authors.lastname: Summers
authors.forename: B L
authors.initials: BL
authors.lastname: Innis
authors.forename: K H
authors.initials: KH
authors.lastname: Eckels
authors.forename: C H
authors.initials: CH
authors.lastname: Hoke
mesh_terms: D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood; D002478:Cells, Cultured; D003715:Dengue / Q000517:prevention & control*; D003716:Dengue Virus / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008887:Military Medicine; D012692:Serial Passage; D016037:Single-Blind Method; D014481:United States; D014613:Vaccines, Attenuated / Q000009:adverse effects; D014765:Viral Vaccines / Q000009:adverse effects; D014766:Viremia
chemical_list: D000914:Antibodies, Viral; D014613:Vaccines, Attenuated; D014765:Viral Vaccines