title: Iron chelation can modulate UVA-induced lipid peroxidation and ferritin expression in human reconstructed epidermis.
abstract: OBJECTIVE
  As ferritin has been identified as an important factor in antioxidant defense in cultured human skin cells, we evaluated UVA-induced lipid hydroperoxides (LPO) production and ferritin expression in reconstructed human epidermis in vitro.
  
  
  RESULTS
  Ferritin is regularly present in the basal layer of unirradiated epidermis both in the human skin in vivo and in the reconstructed human epidermis in vitro. Following acute UVA exposure, ferritin expression increased in basal epidermal cells in both models. Quantitative analysis showed that, in reconstructed human epidermis, LPO and ferritin levels increased linearly with the UVA dose. An iron chelator, OR10141, inhibited these inductions.
  
  
  CONCLUSIONS
  These findings demonstrate that reconstructed human epidermis is a useful in vitro model to study UVA-induced oxidative stress and protection afforded by iron chelators, antioxidants or UVA absorbers.
authors.affiliation: L'Oréal Recherche, Clichy, France. sseite@recherche.loreal.com
authors.forename: Sophie
authors.initials: S
authors.lastname: Seité
authors.forename: Evelyne
authors.initials: E
authors.lastname: Popovic
authors.forename: Marie Paule
authors.initials: MP
authors.lastname: Verdier
authors.forename: Roland
authors.initials: R
authors.lastname: Roguet
authors.forename: Pascal
authors.initials: P
authors.lastname: Portes
authors.forename: Catherine
authors.initials: C
authors.lastname: Cohen
authors.forename: Anny
authors.initials: A
authors.lastname: Fourtanier
authors.forename: Jean Baptiste
authors.initials: JB
authors.lastname: Galey
mesh_terms: D000328:Adult; D046508:Culture Techniques; D004492:Edetic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005293:Ferritins / Q000378:metabolism*; D006801:Humans; D007502:Iron Chelating Agents / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects*; D008297:Male; D018384:Oxidative Stress / Q000528:radiation effects; D011829:Radiation Dosage; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D007502:Iron Chelating Agents; C097359:N,N'-bis(3,4,5-trimethoxybenzyl)ethylenediamine N,N'-diacetic acid; D005293:Ferritins; D004492:Edetic Acid