Effects of 4,4'-diisothiocyanato-stilbene-2,2'-disulfonic acid (DIDS) and chlorpromazine on NO3- transport via anion exchanger in erythrocytes: inertness of DIDS in whole blood.
We examined the effects of chlorpromazine on NO(3)(-) transport between erythrocytes (RBCs) and extracellular fluid. Chlorpromazine (10 microg/ml) did not influence NO(3)(-) movement in both whole blood and RBC suspension. Though an anion exchanger (AE1) inhibitor DIDS (4,4'-diisothiocyanato-stilbene-2,2'-disulfonic acid, 100 microM) did not alter NO(3)(-) movement in whole blood, it inhibited the movement in a concentration-dependent manner in the RBC suspension. The inhibition was abrogated by plasma and albumin concentration-dependently. Our results indicated that chlorpromazine had no effect on NO(3)(-) transport through AE1 and that the inertness of DIDS on AE1 in whole blood is due to interference by albumin in plasma.
Kubota
Kaname
K
Division of Cardiology, Niigata University Graduate School of Medical and Dental Sciences, Japan.
Ishibashi
Takaharu
T
Matsubara
Taku
T
Hori
Tomoyuki
T
Ozaki
Kazuyuki
K
Yamazoe
Masaru
M
Yoshida
Junko
J
Nishio
Matomo
M
Aizawa
Yoshifusa
Y
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology*; D000328:Adult; D001457:Anion Exchange Protein 1, Erythrocyte / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000838:Anions; D014150:Antipsychotic Agents / Q000494:pharmacology*; D001692:Biological Transport; D002746:Chlorpromazine / Q000494:pharmacology*; D004912:Erythrocytes / Q000378:metabolism*; D045604:Extracellular Fluid / Q000378:metabolism; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D009566:Nitrates / Q000097:blood*; D013997:Time Factors
D001457:Anion Exchange Protein 1, Erythrocyte; D000838:Anions; D014150:Antipsychotic Agents; D009566:Nitrates; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D002746:Chlorpromazine