Comparisons of the interactions between two analytes and two structurally similar chiral stationary phases using high-performance liquid chromatography, suspended-state high-resolution magic angle spinning nuclear magnetic resonance and solid-state nuclear magnetic resonance spectroscopy.
A study of the retention behaviour of the enantiomers (R)- and (S)-1,1'-binaphthyl-2,2'-diol as well as (+) and t-)-O,O'-dibenzoyl-tartaric acid was performed using the two chiral stationary phases (CSPs) Kromasil-DMB and Kromasil-TBB. Detailed information about the interactions between the analytes and the two CSPs was obtained from suspended-state HR/MAS transferred NOESY NMR experiments as well as suspended-state HR/MAS 1H NMR titration experiments. Good correlation between the suspended-state HR/MAS NMR experiments and the corresponding HPLC experiments was obtained. This shows that suspended-state HR/MAS NMR as well as solid-state CP/MAS NMR spectroscopy can be used to investigate interactions between stationary phases and analytes under conditions that are similar to those used in HPLC.
Skogsberg
Urban
U
Institut für Organische Chemie, Universität Tübingen, Af der Morgenstelle 18, D-72076 Tübingen, Germany.
Händel
Heidi
H
Sanchez
Domingo
D
Albert
Klaus
K
D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism