title: Imaging of adenovirus-mediated expression of human sodium iodide symporter gene by 99mTcO4 scintigraphy in mice.
abstract: We have evaluated the feasibility of human sodium iodide symporter (hNIS) as a reporter gene in vitro and in vivo. Recombinant adenovirus encoding hNIS (Rad-hNIS) was introduced to FRO cell for 48 hours. Western blotting and 99mTcO4 uptake study revealed functional hNIS expression in the cell. Rad-hNIS was injected to BALB/c mice via tail vein. 99mTcO4 gamma scintigraphy, biodistribution study, and RT-PCR analysis demonstrated a preferential hepatic uptake of 99mTcO4, which was observed for up to one week. Thus, hNIS can be utilized as an effective reporter gene for noninvasive/repeated imaging, in combination with 99mTcO4.
authors.affiliation: Department of Microbiology, University of Ulsan College of Medicine, Seoul, South Korea.
authors.forename: Won Woo
authors.initials: WW
authors.lastname: Lee
authors.forename: Dae Hyuk
authors.initials: DH
authors.lastname: Moon
authors.forename: So Yeon
authors.initials: SY
authors.lastname: Park
authors.forename: Jungsun
authors.initials: J
authors.lastname: Jin
authors.forename: Sung Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Heuiran
authors.initials: H
authors.lastname: Lee
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D005786:Gene Expression Regulation / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D007668:Kidney / Q000000981:diagnostic imaging / Q000196:embryology / Q000378:metabolism*; D008297:Male; D008657:Metabolic Clearance Rate; D051379:Mice; D008807:Mice, Inbred BALB C; D009928:Organ Specificity; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D011994:Recombinant Proteins / Q000378:metabolism; D013670:Sodium Pertechnetate Tc 99m / Q000493:pharmacokinetics*; D027981:Symporters / Q000235:genetics / Q000378:metabolism*; D013961:Thyroid Gland / Q000000981:diagnostic imaging / Q000378:metabolism*; D014018:Tissue Distribution; D014170:Transformation, Genetic / Q000235:genetics; D000099209:Sodium-Iodide Symporters
chemical_list: D019275:Radiopharmaceuticals; D011994:Recombinant Proteins; D013670:Sodium Pertechnetate Tc 99m; D027981:Symporters; D000099209:Sodium-Iodide Symporters