Analysis of genetic relationships and antimicrobial susceptibility of Escherichia coli isolated from Clethrionomys glareolus.
Eleven strains of Escherichia coli were isolated from 54 bank voles living in the LomZa Landscape Park of the Narew River Valley, indicating that E. coli is not common in the alimentary tract of these mammals. On the basis of pulsed-field gel electrophoresis and computer-assisted analysis, the isolates were grouped into six genotypes at similarities of 39%. Chromosome length of E. coli under study differed by as much as 900 kb, ranging 2.7-3.6 Mb. All strains were susceptible to amikacin and ciprofloxacin, whereas, for tetracycline, streptomycin, ampicillin, and cefonicid, different results were noted. No differences were detected among the plasmid complements of eight strains (73%), for which plasmid profiles revealed the presence of two plasmidic bands. One, three and four plasmids were observed in a plasmid pattern of single isolates. The observation from the study indicated the high genetic polymorphism among the isolates recovered from the animals of one species living in the same environment.
Swiecicka
Izabela
I
Department of Microbiology, Institute of Biology, University of Białystok, Swierkowa 20B, 15-950 Białystok, Poland. izabelas@uwb.edu.pl
Buczek
Jan
J
Iwaniuk
Agnieszka
A
D000818:Animals; D003411:Arvicolinae / Q000382:microbiology*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D016680:Genome, Bacterial; D005838:Genotype; D005843:Geography; D008826:Microbial Sensitivity Tests; D010802:Phylogeny; D011044:Poland
D004269:DNA, Bacterial