title: Transforming growth factor-beta1 accelerates bone bonding to a blended calcium phosphate ceramic coating: a dose-response study.
abstract: In vivo strain measurements can facilitate the study of the bone remodeling response to loading and load changes. Calcium phosphate ceramic (CPC) coatings have been used to attach strain gauges to bone for extended periods of time, but require up to 12 weeks for adequate CPC-to-bone bonding. Transforming growth factor beta 1 (TGF-beta1), an osteoinductive growth factor, was used as a surface enhancement to accelerate bone growth and bonding to CPC particles. The aim of this study was to find an optimal dosage of TGF-beta1 to accelerate the attachment process. CPC-coated strain gauges were enhanced with doses of 0.5, 1.0, or 2.0 microg of TGF-beta1 per gauge. Gauges were placed on the femora of dogs, which were exercised daily and fed ad libitum. After 3, 6, and 12 weeks, gauge attachment was quantitatively assessed using mechanical testing and histomorphometry. Gauge attachment was also qualitatively assessed using back scatter electron microscopy. Agreement of the mechanical test results with both the back scatter electron microscopy images and histomorphometry results showed that the 1.0 microg per gauge dose of TGF-beta1 is an optimal dose to accelerate bone formation and attachment to CPC-coated strain gauges.
authors.affiliation: Department of Orthopaedic Surgery, College of Medicine, University of Arizona, Tucson, Arizona 85724-5194, USA. szivek@arizona.edu
authors.forename: John A
authors.initials: JA
authors.lastname: Szivek
authors.forename: Ehren R
authors.initials: ER
authors.lastname: Nelson
authors.forename: Steven D
authors.initials: SD
authors.lastname: Hajdu
authors.forename: Kelly
authors.initials: K
authors.lastname: Yablonski
authors.forename: Don W
authors.initials: DW
authors.lastname: DeYoung
mesh_terms: D000818:Animals; D001843:Bone Cements; D001861:Bone Regeneration / Q000187:drug effects*; D002130:Calcium Phosphates; D002516:Ceramics / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D005269:Femur / Q000502:physiology / Q000601:surgery; D020007:Implants, Experimental; D007700:Kinetics; D008297:Male; D013314:Stress, Mechanical; D016212:Transforming Growth Factor beta / Q000008:administration & dosage / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1
chemical_list: D001843:Bone Cements; D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1