Expression of a foot-and-mouth disease virus immunodominant epitope by a filamentous bacteriophage vector.
We described the construction of a recombinant filamentous phage displaying on its surface the immunodominant site of VP1 protein of foot-and-mouth disease virus (FMDV). The coding sequence was inserted at the amino-terminus of the major coat protein pVIII via a spacer. The hybrid phage proved to be antigenic as it was recognized by polyclonal and monoclonal anti FMDV sera. In two experiments involving immunisation of guinea-pigs with the recombinant phage, a low antibody response was generated. This suggests a possible role for phage displayed peptides in inducing anti FMDV immunity and the possibility of further development is discussed.
Kim
Y J
YJ
Laboratoire d'études et de recherches en pathologie animale et zoonoses, AFSSA, Maisons-Alfort, France.
Lebreton
F
F
Kaiser
C
C
Crucière
C
C
Rémond
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D029544:Foot-and-Mouth Disease Virus / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D005822:Genetic Vectors / Q000235:genetics; D006168:Guinea Pigs; D007106:Immune Sera / Q000276:immunology; D016056:Immunodominant Epitopes / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D017912:Inovirus / Q000235:genetics*; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D016366:Open Reading Frames / Q000235:genetics; D011817:Rabbits
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D007106:Immune Sera; D016056:Immunodominant Epitopes