Methylocella tundrae sp. nov., a novel methanotrophic bacterium from acidic tundra peatlands.
A novel species, Methylocella tundrae, is proposed for three methanotrophic strains (T4T, TCh1 and TY1) isolated from acidic Sphagnum tundra peatlands. These strains are aerobic, Gram-negative, non-motile, dinitrogen-fixing rods that possess a soluble methane monooxygenase and utilize the serine pathway for carbon assimilation. Strains T4T, TCh1 and TY1 are moderately acidophilic organisms capable of growth between pH 4.2 and 7.5 (optimum 5.5-6.0) and between 5 and 30 degrees C (optimum 15 degrees C). The major phospholipid fatty acid is 18:1omega7c. The DNA G+C content of strain T4T is 63.3 mol%. The three strains possess almost identical 16S rRNA gene sequences and are most closely related to two previously identified species of Methylocella, Methylocella palustris (97% similarity) and Methylocella silvestris (97.5% similarity). DNA-DNA hybridization values of strain T4T with Methylocella palustris KT and Methylocella silvestris BL2T were respectively 27 and 36%. Thus, the tundra strains represent a novel species, for which the name Methylocella tundrae sp. nov. is proposed. Strain T4T (=DSM 15673T=NCIMB 13949T) is the type strain.
Dedysh
Svetlana N
SN
Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia.
Berestovskaya
Yulia Y
YY
Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia.
Vasylieva
Lina V
LV
Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia.
Belova
Svetlana E
SE
Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia.
Khmelenina
Valentina N
VN
Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia.
Suzina
Natalia E
NE
Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia.
Trotsenko
Yuri A
YA
Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia.
Liesack
Werner
W
Max-Planck-Institut für terrestrische Mikrobiologie, D-35043 Marburg, Germany.
Zavarzin
George A
GA
Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia.
D002244:Carbon / Q000378:metabolism; D003081:Cold Climate; D004798:Enzymes / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010802:Phylogeny; D012230:Rhizobiaceae / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification; D012426:Russia; D012694:Serine / Q000378:metabolism; D012988:Soil Microbiology
D004798:Enzymes; D005227:Fatty Acids; D012694:Serine; D002244:Carbon