Effect of 1-oxo-5beta, 6beta-epoxy-witha-2-ene-27-ethoxy-olide isolated from the roots of Withania somnifera on stress indices in Wistar rats.
OBJECTIVE
Isolation of biologically active fractions and compounds from the roots of Withania somnifera, a plant used extensively as a constituent of rasayana, in Ayurveda and to test their adaptogenic activity on stress indices using the cold-hypoxia-restraint (C-H-R) model.


METHODS
Bioactivity-guided fractionation of an aqueous extract of the roots of Withania somnifera led to the isolation of a new species of withanolide 1-oxo-5beta, 6beta-epoxy-witha-2-ene-27-ethoxy-olide. Structure elucidation, was carried out using proton nuclear magnetic resonance, infrared (IR), ultraviolet (UV), and mass spectroscopic analysis. Stress-related indices were evaluated, namely serum creatine phosphokinase (CPK) activity, serum lactate dehydrogenase (LDH) activity, serum corticosterone levels, and serum lipid peroxidation (LPO) levels.


RESULTS
There was a significant decrease in a serum CPK, LDH, and LPO levels in animals pretreated with (1) fraction-I (20 mg/kg body weight), (2) 1-oxo-5beta, 6beta-epoxy-witha-2-ene-27-ethoxy-olide (2.5 mg/kg body weight) in comparison to control when subjected to C-H-R stress.


CONCLUSIONS
The results show that the a new species of withanolide, 1-oxo-5beta, 6beta-epoxy-witha-2-ene-27-ethoxy-olide (compound-1) could prove to be an effective agent to counteract C-H-R stress.
Kaur
Parvinder
P
Dr. B.R. Ambedkar Center for Biomedical Research, University of Delhi, Delhi 110-007, India. acbdu@hotmail.com
Sharma
Meenakshi
M
Mathur
Sheenu
S
Tiwari
Manisha
M
Divekar
Harish M
HM
Kumar
Ratan
R
Srivastava
Kaushal K
KK
Chandra
Ramesh
R
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D003080:Cold Temperature; D003345:Corticosterone / Q000097:blood; D003402:Creatine Kinase / Q000097:blood; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004875:Ergosterol / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology*; D000860:Hypoxia; D007770:L-Lactate Dehydrogenase / Q000097:blood; D015227:Lipid Peroxidation; D009682:Magnetic Resonance Spectroscopy; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical; D013055:Spectrophotometry, Infrared; D013315:Stress, Psychological / Q000139:chemically induced / Q000188:drug therapy*; D032341:Withania / Q000737:chemistry
C485442:1-oxo-5,6-epoxy-witha-2-ene-27-ethoxyolide; D010936:Plant Extracts; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; D003345:Corticosterone; D004875:Ergosterol