Monitoring the formation of Maillard reaction products of glucosamine with fibrinogen and human serum albumin using capillary electrophoresis.
Formation of Maillard reaction products (MRP) of glucosamine (GlcN) with fibrinogen and human serum albumin (HSA), under simulated physiological conditions, was detected by fluorescence (excitation/emission: 340/420 nm) and UV/Vis (max. 275 nm) spectroscopy. The application of polyacrylamide gel electrophoresis demonstrated the generation of high-molecular-weight fibrinogen and HSA MRP by GlcN. A simple and rapid capillary electrophoresis method was developed to separate MRP formed by the reaction of GlcN with proteins from GlcN autocondensation products.
Zhang
Xinfeng
X
Department of Chemistry, University of Rhode Island, Kingston, RI 02881, USA.
Ma
Yue
Y
Liu
Hong
H
de Sa
Paula F G
PF
Brown
Phyllis R
PR
Dain
Joel A
JA
D019075:Electrophoresis, Capillary / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen / Q000378:metabolism*; D005944:Glucosamine / Q000378:metabolism*; D017127:Glycation End Products, Advanced / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D015416:Maillard Reaction; D012709:Serum Albumin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D017127:Glycation End Products, Advanced; D012709:Serum Albumin; D005340:Fibrinogen; D005944:Glucosamine