Establishment and characterization of an angiosarcoma-derived cell line, AS-M.
A novel human endothelial cell line, AS-M, has been established from a cutaneous angiosarcoma on the scalp. The cells expressing platelet endothelial cell adhesion molecule-1 (CD31) were isolated using magnetic beads and subsequently cultured for a year. To date, the cells have undergone more than 100 population doublings (PDs). The AS-M cells manifested endothelial characteristics, such as active uptake of acetylated low-density lipoprotein labeled with 1,1'-dioctadecyl 3,3,3',3'-tetramethylindocarbocyanine perchlorate (Dil-Ac-LDL), capacity to bind the Ulex europeaus agglutin-I (UEA-I), and expression of von Willebrand factor (vWF) and CD31. The single cell-derived clone, AS-M.5, showed a constitutive expression of CD31, vWF, angiotensin-converting enzyme (ACE), endoglin (CD105), and the endothelial cell receptor tyrosine kinases KDR and Tie-1. Similarly to freshly isolated endothelial cells, the AS-M.5 responded to induction by bacterial lipopolysaccharide (LPS) by increased transcription of cell adhesion molecules and cytokines. The AS-M.5 cultures required endothelial growth supplements for optimal growth and long-term propagation in vitro. However, in contrast to normal endothelial cells, p53 gene products were detected in nuclei of AS-M.5 cells. Cytogenetic analyses consistently revealed a hypodiploid karyotype with complete loss of one homologue of several chromosomes and a homogeneous pattern of distinct karyotypic changes. Although the AS-M.5 presented characteristics suggestive of tumor cells, they did not develop into tumors when inoculated subcutaneously into nude mice. The cell line AS-M.5 could be a useful model system to study endothelial pathobiology in vitro.
Krump-Konvalinkova
Vera
V
Institute of Pathology, Johannes Gutenberg University Mainz, Germany.
Bittinger
Fernando
F
Olert
Jürgen
J
Bräuninger
Wolfgang
W
Brunner
Joachim
J
Kirkpatrick
C James
CJ
D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002455:Cell Division; D045744:Cell Line, Tumor; D016207:Cytokines / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D019085:Fluorescent Antibody Technique, Direct; D006394:Hemangiosarcoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007621:Karyotyping; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction
D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D016207:Cytokines; D008077:Lipoproteins, LDL