In vivo filtering of in vitro expression data reveals MyoD targets.
A published set of downstream targets of MyoD defined in a well-controlled in vitro experiment was filtered for relevance to muscle regeneration using a 27-time-point in vivo murine regeneration series. Using interactive hierarchical and Bayes soft clustering, only a minority of the targets defined in vitro can be confirmed in vivo (approximately 50% of induced transcripts, and none of repressed transcripts). This approach provided strong support that 18 targets including of MyoD are biologically relevant during myoblast differentiation.
Zhao
Po
P
Research Center for Genetic Medicine, Children's National Medical Center, Washington, DC 20010, USA.
Seo
Jinwook
J
Wang
Zuyi
Z
Wang
Yue
Y
Shneiderman
Ben
B
Hoffman
Eric P
EP
D000818:Animals; D016000:Cluster Analysis; D005786:Gene Expression Regulation; D051379:Mice; D018482:Muscle, Skeletal / Q000502:physiology*; D017570:MyoD Protein / Q000235:genetics / Q000502:physiology*; D012038:Regeneration
D017570:MyoD Protein