title: Antibody detection of SARS-CoV spike and nucleocapsid protein.
abstract: Early detection and identification of SARS-CoV-infected patients and actions to prevent transmission are absolutely critical to prevent another SARS outbreak. Antibodies that specifically recognize the SARS-CoV spike and nucleocapsid proteins may provide a rapid screening method to allow accurate identification and isolation of patients with the virus early in their infection. For this reason, we raised peptide-induced polyclonal antibodies against SARS-CoV spike protein and polyclonal antibodies against SARS-CoV nucleocapsid protein using 6x His nucleocapsid recombinant protein. Western blot analysis and immunofluorescent staining showed that these antibodies specifically recognized SARS-CoV.
authors.affiliation: Department of Medical Research, Mackay Memorial Hospital, ROC, Taipei, Taiwan.
authors.forename: Mau-Sun
authors.initials: MS
authors.lastname: Chang
authors.forename: Yen-Ta
authors.initials: YT
authors.lastname: Lu
authors.forename: Shin-Tsung
authors.initials: ST
authors.lastname: Ho
authors.forename: Chao-Chih
authors.initials: CC
authors.lastname: Wu
authors.forename: Tsai-Yin
authors.initials: TY
authors.lastname: Wei
authors.forename: Chia-Ju
authors.initials: CJ
authors.lastname: Chen
authors.forename: Yun-Ting
authors.initials: YT
authors.lastname: Hsu
authors.forename: Po-Chen
authors.initials: PC
authors.lastname: Chu
authors.forename: Ching-Hsin
authors.initials: CH
authors.lastname: Chen
authors.forename: Jien-Ming
authors.initials: JM
authors.lastname: Chu
authors.forename: Ya-Lin
authors.initials: YL
authors.lastname: Jan
authors.forename: Chia-Chien
authors.initials: CC
authors.lastname: Hung
authors.forename: Chi-Chen
authors.initials: CC
authors.lastname: Fan
authors.forename: Yuh-Cheng
authors.initials: YC
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D015153:Blotting, Western; D005455:Fluorescent Antibody Technique; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000737:chemistry / Q000276:immunology*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology / Q000302:isolation & purification*; D064370:Spike Glycoprotein, Coronavirus; D013347:Subcellular Fractions; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology*
chemical_list: D008562:Membrane Glycoproteins; D019590:Nucleocapsid Proteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins