Genomic instability in oral squamous cell carcinoma: relationship to betel-quid chewing.
Genomic instability in repeated DNA sequences is exhibited by a variety of cancer types, including oral squamous cell carcinoma. Exposure to carcinogenic compounds may further increase the instability. We have used Inter-Simple Sequence Repeat (Inter-SSR) PCR methodology to detect genetic alterations in 37 oral cancer patients who had chewed betel-quid. Thirty-eight percent of DNA from tumors had genomic alterations in the sequences flanked by (CA)(8) and (GT)(8) repeats. Patients with tumor DNAs harboring genomic alterations had a two-fold higher consumption of betel-quid than patients without alterations in tumor DNA. Matched normal and tumor DNAs were also screened for microsatellite instability where four patients (10.8%) showed alterations in at least one microsatellite marker but there was no relationship between this phenotype and betel-quid chewing. These data indicate that exposure to carcinogens present in the betel-quid may contribute to genomic instability detected by inter-SSR PCR in a subset of oral tumors.
Zienolddiny
Shanbeh
S
International Agency for Research on Cancer 150, Cours Albert Thomas, 69372 Lyon Cedex 08, France.
Aguelon
Anne-Marie
AM
Mironov
Nikolai
N
Mathew
Babu
B
Thomas
Gigi
G
Sankaranarayanan
Rengaswamy
R
Yamasaki
Hiroshi
H
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001114:Areca / Q000009:adverse effects*; D002294:Carcinoma, Squamous Cell / Q000209:etiology / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D042822:Genomic Instability; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D008409:Mastication; D018895:Microsatellite Repeats; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000209:etiology / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods
D004273:DNA, Neoplasm