Flagellar quiescence in Chlamydomonas: Characterization and defective quiescence in cells carrying sup-pf-1 and sup-pf-2 outer dynein arm mutations.
Chlamydomonas reinhardtii can use their flagella for two distinct types of movement: swimming through liquid or gliding on a solid substrate. Cells switching from swimming to gliding motility undergo a reversible flagellar quiescence. This phenomenon appears to involve the outer dynein arms, since mutants having altered outer arm beta and gamma dyneins (sup-pf-1 and sup-pf-2) show a diminished ability to quiesce. Sup-pf-1 and sup-pf-2 were originally isolated as gain-of-function mutations that suppress the flagellar paralysis resulting from radial spoke or central pair defects. Defective quiescence is also a gain-of-function phenomenon, as cells completely lacking outer arm heavy chains show a normal quiescence phenotype. These data suggest that regulation of outer arm dynein activity is essential for flagellar quiescence and furthermore that regulation of quiescence involves a signal transduction pathway that shares elements with the radial spoke/central pair system.
Mitchell
Beth Ferro
BF
Department of Biology, Le Moyne College, Syracuse, New York 13214-1399. mitchell@lemoyne.edu
Grulich
Laurie E
LE
Mader
Michelle M
MM
D000818:Animals; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D016825:Chlamydomonas reinhardtii / Q000235:genetics / Q000502:physiology*; D004398:Dyneins / Q000235:genetics / Q000502:physiology*; D005407:Flagella / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D015398:Signal Transduction / Q000502:physiology
D004398:Dyneins