Haemophilus ducreyi strain ATCC 27722 contains a genetic element with homology to the vibrio RS1 element that can replicate as a plasmid and confer NAD independence on haemophilus influenzae.
The nucleotide sequence of pNAD1, a plasmid from Haemophilus ducreyi identified on the basis of its ability to confer NAD independence on Actinobacillus pleuropneumoniae and H. influenzae, has been determined. In addition to containing the nadV gene, the plasmid contains homologues of the rstR and rstA genes, genes encoding repressor and replication proteins, respectively, in the Vibrio CTXphi and the Vibrio RS1 element, suggesting a single-stranded bacteriophage origin for pNAD1. Tandem copies of the plasmid are integrated into the H. ducreyi 35000HP genome.
Munson
Robert S
RS
Division of Molecular Medicine, Children's Research Institute, and Department of Pediatrics, The Ohio State University, Columbus, Ohio 43205, USA. munsonr@pediatrics.ohio-state.edu
Zhong
Huachun
H
Mungur
Rachna
R
Ray
William C
WC
Shea
Robin J
RJ
Mahairas
Gregory G
GG
Mulks
Martha H
MH
D000595:Amino Acid Sequence; D016680:Genome, Bacterial; D006191:Haemophilus ducreyi / Q000235:genetics*; D006193:Haemophilus influenzae / Q000235:genetics*; D008969:Molecular Sequence Data; D009243:NAD / Q000235:genetics*; D016366:Open Reading Frames; D010957:Plasmids; D014733:Vibrio / Q000235:genetics*
D009243:NAD