The mechanisms of vesicle budding and fusion.
Genetic and biochemical analyses of the secretory pathway have produced a detailed picture of the molecular mechanisms involved in selective cargo transport between organelles. This transport occurs by means of vesicular intermediates that bud from a donor compartment and fuse with an acceptor compartment. Vesicle budding and cargo selection are mediated by protein coats, while vesicle targeting and fusion depend on a machinery that includes the SNARE proteins. Precise regulation of these two aspects of vesicular transport ensures efficient cargo transfer while preserving organelle identity.
Bonifacino
Juan S
JS
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA. juan@helix.nih.gov
Glick
Benjamin S
BS
D000818:Animals; D001692:Biological Transport; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D008561:Membrane Fusion; D008565:Membrane Proteins / Q000502:physiology*; D008954:Models, Biological; D008958:Models, Molecular; D015388:Organelles / Q000502:physiology; D050600:SNARE Proteins; D012441:Saccharomyces cerevisiae / Q000502:physiology / Q000648:ultrastructure; D013696:Temperature; D033921:Vesicular Transport Proteins
D008565:Membrane Proteins; D050600:SNARE Proteins; D033921:Vesicular Transport Proteins