Involvement of adenosine A1 receptors in forced walking stress-induced analgesia in mice.
We investigated the involvement of adenosine receptors on forced walking stress-induced analgesia using a formalin-induced paw-licking test in male mice. Exposure to forced walking stress for 6 h showed stress-induced analgesia in the second phase (10-30 min), but not in the first phase (0-10 min). In the second phase, forced walking stress-induced analgesia was blocked by theophylline, a nonselective adenosine-receptor antagonist and DPCPX, an adenosine A1-receptor antagonist, but not ZM 241385, an adenosine A2A-receptor antagonist. These findings suggest that adenosine A1 receptors are involved in the analgesic mechanism activated by the forced walking stress.
Furuta
S
S
Second Department of Physiology, Showa University, School of Medicine, Tokyo, Japan.
Onodera
K
K
Kumagai
M
M
Honma
I
I
Miyazaki
S
S
Sato
T
T
Sakurada
S
S
D058916:Adenosine A1 Receptor Antagonists; D000698:Analgesia; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology*; D005557:Formaldehyde / Q000633:toxicity; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010146:Pain / Q000139:chemically induced / Q000378:metabolism*; D043682:Receptor, Adenosine A1 / Q000378:metabolism*; D013315:Stress, Psychological / Q000378:metabolism* / Q000503:physiopathology; D013806:Theophylline / Q000494:pharmacology; D014227:Triazines / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology; D016138:Walking; D014970:Xanthines / Q000494:pharmacology
D058916:Adenosine A1 Receptor Antagonists; D043682:Receptor, Adenosine A1; D014227:Triazines; D014230:Triazoles; D014970:Xanthines; C097270:ZM 241385; D005557:Formaldehyde; C051360:1,3-dipropyl-8-cyclopentylxanthine; D013806:Theophylline