title: In vivo filtering of in vitro expression data reveals MyoD targets.
abstract: A published set of downstream targets of MyoD defined in a well-controlled in vitro experiment was filtered for relevance to muscle regeneration using a 27-time-point in vivo murine regeneration series. Using interactive hierarchical and Bayes soft clustering, only a minority of the targets defined in vitro can be confirmed in vivo (approximately 50% of induced transcripts, and none of repressed transcripts). This approach provided strong support that 18 targets including of MyoD are biologically relevant during myoblast differentiation.
authors.affiliation: Research Center for Genetic Medicine, Children's National Medical Center, Washington, DC 20010, USA.
authors.forename: Po
authors.initials: P
authors.lastname: Zhao
authors.forename: Jinwook
authors.initials: J
authors.lastname: Seo
authors.forename: Zuyi
authors.initials: Z
authors.lastname: Wang
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Ben
authors.initials: B
authors.lastname: Shneiderman
authors.forename: Eric P
authors.initials: EP
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D005786:Gene Expression Regulation; D051379:Mice; D018482:Muscle, Skeletal / Q000502:physiology*; D017570:MyoD Protein / Q000235:genetics / Q000502:physiology*; D012038:Regeneration
chemical_list: D017570:MyoD Protein