title: Crystallization and preliminary X-ray diffraction analysis of a dihaem cytochrome c peroxidase from Paracoccus denitrificans.
abstract: Cytochrome c peroxidase was isolated from Paracoccus denitrificans and purified to homogeneity in three steps prior to crystallization. Two different diffraction-quality crystal forms were obtained by the hanging-drop vapour-diffusion method using a number of screening conditions. The best (needle-shaped) crystal form is suitable for structural studies and was grown from solutions containing 20% PEG 8000, 0.1 M Tris pH 8.5 and 0.2 M MgCl(2). Crystals grew to a maximum length of approximately 0.7 mm and belong to the primitive monoclinic space group P2(1), with unit-cell parameters a = 78.3, b = 51.0, c = 167.2 A, beta = 97.9 degrees. After a dehydration step and extensive optimization of the cryocooling conditions, a complete data set was collected to 2.2 A from a native crystal of the fully oxidized form of the enzyme using synchrotron radiation.
authors.affiliation: Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, England.
authors.forename: Aude
authors.initials: A
authors.lastname: Echalier
authors.forename: Celia F
authors.initials: CF
authors.lastname: Goodhew
authors.forename: Graham W
authors.initials: GW
authors.lastname: Pettigrew
authors.forename: Vilmos
authors.initials: V
authors.lastname: Fülöp
mesh_terms: D003461:Crystallography; D003578:Cytochrome-c Peroxidase / Q000737:chemistry*; D006418:Heme / Q000737:chemistry*; D010100:Oxygen / Q000378:metabolism; D010231:Paracoccus denitrificans / Q000201:enzymology*; D011092:Polyethylene Glycols / Q000737:chemistry; D011487:Protein Conformation; D017356:Synchrotrons; D013696:Temperature; D013997:Time Factors; D014961:X-Ray Diffraction
chemical_list: D011092:Polyethylene Glycols; D006418:Heme; D003578:Cytochrome-c Peroxidase; D010100:Oxygen