title: [Effect of low-intensity microwave radiation on proliferation of cultured epithelial cells of rabbit lens].
abstract: OBJECTIVE
  To compare the effects of different doses of microwave on the proliferative activity and cell cycle of cultured epithelial cells of rabbit lens, and to investigate the limit tolerant of microwave exposure.
  
  
  METHODS
  Cultured epithelial cells of rabbit lens were exposed to microwave radiation with frequency of 2,450 MHz and power density of 0.10, 0.25, 0.50, 1.00, 2.00 mW/cm(2) for 8 h in vitro. HE staining was used to observe the morphological changes of lens epithelial cells, the proliferative activity and cell cycle were measured by MTT assay and PI fluorescent staining.
  
  
  RESULTS
  8 h after radiation, 0.50, 1.00 and 2.00 mW/cm(2) microwave could decrease the proliferation of lens epithelial cells, make the cells disordered arrangement, shrinkage, detachment, and inhibit the synthesis of cell DNA. The percentage of G(0)/G(1) phase cells were 71.95% +/- 2.12%, 75.68% +/- 3.35% and 82.40% +/- 8.68% respectively, which were higher than that in control group (61.68% +/- 5.76%, P < 0.05 or P < 0.01). The percentage of S phase cells were 19.32% +/- 3.07%, 16.08% +/- 4.91% and 12.98% +/- 8.08% respectively, which were lower than that in control group (28.05% +/- 5.12%, P < 0.05 or P < 0.01). No obvious changes could be detected in 0.10, 0.25 mW/cm(2) microwave groups (P > 0.05).
  
  
  CONCLUSIONS
  Microwave exceeding 0.50 mW/cm(2) may make injury to lens epithelial cells after 8 hour radiation, which may be related to the effect of microwave radiation on cell cycle.
authors.affiliation: Department of Ophthalmology, The Second Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310009, China.
authors.forename: Kai-jun
authors.initials: KJ
authors.lastname: Wang
authors.forename: Ke
authors.initials: K
authors.lastname: Yao
authors.forename: De-qiang
authors.initials: DQ
authors.lastname: Lu
authors.forename: Huai
authors.initials: H
authors.lastname: Jiang
authors.forename: Jian
authors.initials: J
authors.lastname: Tan
authors.forename: Wen
authors.initials: W
authors.lastname: Xu
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000528:radiation effects*; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D007908:Lens, Crystalline / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D008872:Microwaves; D011817:Rabbits
chemical_list: D004247:DNA