Colony aggregation and biofilm formation in xylem chemistry-based media for Xylella fastidiosa.
Two chemically defined media based on xylem fluid chemistry were developed for Xylella fastidiosa. These media were tested and compared to chemically defined media XDM2, XDM4 and XF-26. New media were evaluated for the Pierce's disease (PD) strain UCLA-PD. Our media either was similar to the concentration of some amino acids found in the xylem fluid of the PD-susceptible Vitis vinifera cv. Chardonnay (medium CHARD2) or incorporated the tripeptide glutathione found in xylem fluid composition (medium 3G10-R). CHARD2 and 3G10-R are among the simplest chemically defined media available. Xylem fluid chemistry-based media supported X. fastidiosa growth and especially stimulated aggregation and biofilm formation.
Leite
Breno
B
North Florida Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, 155 Research Road, Quincy, FL 32351, USA. bleite@mail.ufl.edu
Andersen
Peter Craig
PC
Ishida
Maria Lucia
ML
D000596:Amino Acids / Q000378:metabolism; D001422:Bacterial Adhesion; D001431:Bacteriological Techniques; D018441:Biofilms / Q000254:growth & development*; D003470:Culture Media / Q000737:chemistry; D005978:Glutathione / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D027843:Vitis / Q000737:chemistry / Q000382:microbiology; D044167:Xylella / Q000254:growth & development* / Q000502:physiology
D000596:Amino Acids; D003470:Culture Media; D005978:Glutathione