Prevalence of human herpesvirus-6B in Korean hematopoietic stem cell transplantation recipients.
Human herpesvirus-6 (HHV-6) is a major pathogen associated with diseases of recipients of hematopoietic stem cell transplants (HSCT). We have isolated HHV-6 in Korean HSCT recipients and carried out a prospective investigation of its prevalence. We obtained peripheral blood from HSCT recipients who had signs of HHV-6 infection. Cord blood mononuclear cells (CBMC) and Sup-T1 cells were used to culture the HHV-6. Indirect immunofluorescence assays (IFA), and the polymerase chain reaction (PCR) were employed to detect HHV-6. The prevalence of HHV-6 infection in HSCT recipients was calculated on the basis of the PCR results. HHV-6 was isolated from four clinical samples. After culturing the HHV-6 in CBMC, the standard strain and the four clinical isolates were propagated in Sup-T1 cells. The infected cells became grossly enlarged and multinucleate after 7-21 days. The virus was identified primarily on the basis of the morphological changes of the cultured cells, and confirmed by specific IFA with monoclonal antibody to HHV-6. HHV-6 was detected in each sample by PCR with primers specific for the major immediate early gene. Sequencing of the standard strain and PCR products confirmed identification of the HHV-6B variant. By PCR we detected 415 instances of HHV-6 in 3966 samples (14.6% of peripheral blood mononuclear cells and 6.3% of sera), and HHV-6 DNAemia was most frequent from the second to the fourth week after HSCT.
Lee
Dong Gun
DG
Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea.
Park
Sang Tae
ST
Choi
Su Mi
SM
Kim
Seung Han
SH
Choi
Jung Hyun
JH
Yoo
Jin Hong
JH
Park
Seung Won
SW
Lee
Gyu Cheol
GC
Paik
Soon Young
SY
Shin
Wan Shik
WS
Kim
Chun Choo
CC
D001483:Base Sequence; D004279:DNA, Viral / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D018380:Hematopoietic Stem Cell Transplantation; D015654:Herpesvirus 6, Human / Q000235:genetics; D006801:Humans; D007723:Korea / Q000453:epidemiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015995:Prevalence; D019349:Roseolovirus Infections / Q000453:epidemiology*
D004279:DNA, Viral