title: Naringenin from Citrus junos has an inhibitory effect on acetylcholinesterase and a mitigating effect on amnesia.
abstract: This study was performed to identify safe and more effective acetylcholinesterase (AChE) inhibitors in the treatment of Alzheimer's disease. The total methanol extract of Citrus junos had a significant inhibitory effect on AChE in vitro. By sequential fractionation of C.junos, the active component was finally identified as naringenin. Naringenin inhibited AChE activity in a dose-dependent manner. In this study, we also evaluated the anti-amnesic activity of naringenin, a major flavanone constituent isolated from C. junos, in vivo using ICR mice with amnesia induced by scopolamine (1 mg/kg body weight). Naringenin, when administered to mice at 4.5 mg/kg body weight, significantly ameliorated scopolamine-induced amnesia as measured in both the passive avoidance and the Y-maze test. These results suggest that naringenin may be a useful chemopreventive agent against Alzheimer's disease.
authors.affiliation: Graduate School of Biotechnology, Korea University, Seoul, Korea.
authors.forename: Ho Jin
authors.initials: HJ
authors.lastname: Heo
authors.forename: Mi-Jeong
authors.initials: MJ
authors.lastname: Kim
authors.forename: Jung-Min
authors.initials: JM
authors.lastname: Lee
authors.forename: Soo Jung
authors.initials: SJ
authors.lastname: Choi
authors.forename: Hong-Yon
authors.initials: HY
authors.lastname: Cho
authors.forename: Bumshik
authors.initials: B
authors.lastname: Hong
authors.forename: Hye-Kyung
authors.initials: HK
authors.lastname: Kim
authors.forename: Eunki
authors.initials: E
authors.lastname: Kim
authors.forename: Dong-Hoon
authors.initials: DH
authors.lastname: Shin
mesh_terms: D000110:Acetylcholinesterase / Q000032:analysis; D000647:Amnesia / Q000139:chemically induced / Q000188:drug therapy*; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D001522:Behavior, Animal / Q000187:drug effects; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D002855:Chromatography, Thin Layer; D002957:Citrus / Q000737:chemistry*; D044950:Flavanones / Q000494:pharmacology* / Q000627:therapeutic use*; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D000432:Methanol; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D012601:Scopolamine; D012997:Solvents
chemical_list: D002800:Cholinesterase Inhibitors; D044950:Flavanones; D010936:Plant Extracts; D012997:Solvents; D012601:Scopolamine; D000110:Acetylcholinesterase; C005273:naringenin; D000432:Methanol