title: Reversed-phase liquid chromatographic method for the determination of ochratoxin A in wine.
abstract: In this paper, we propose a new, rapid, highly sensitive and reproducible RP-HPLC-FLD method for the detection of ochratoxin A (OTA) in wine, by directly injecting the liquid in the chromatographic system without any extraction or clean-up. An alkaline mobile phase (NH4Cl:CH-CN 85:15 (v/v), 20 mM, pH 9.8) was used to obtain a distinct fluorescence enhancement. This improvement allows to reach, without an immunoaffinity clean-up or concentration, a detection limit of 0.05 ng/ml, which is similar to those commonly obtained after immunoaffinity purification and acidic elution. The method was statistically validated and directly applied to a series of wine samples.
authors.affiliation: Dipartimento di Chimica Organica e Industriale, Università degli Studi di Parma, Parco Area delle Scienze 17/A, I-43100 Parma, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Dall'Asta
authors.forename: G
authors.initials: G
authors.lastname: Galaverna
authors.forename: A
authors.initials: A
authors.lastname: Dossena
authors.forename: R
authors.initials: R
authors.lastname: Marchelli
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009793:Ochratoxins / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014920:Wine / Q000032:analysis*
chemical_list: D009793:Ochratoxins; C025589:ochratoxin A