title: Multiplexed toxin analysis using four colors of quantum dot fluororeagents.
abstract: Quantum dots (QDs) have the potential to simplify the performance of multiplexed analysis. In this work, we prepared bioinorganic conjugates made with highly luminescent semiconductor nanocrystals (CdSe-ZnS core-shell QDs) and antibodies to perform multiplexed fluoroimmunoassays. Sandwich immunoassays for the detection of cholera toxin, ricin, shiga-like toxin 1, and staphylococcal enterotoxin B were performed simultaneously in single wells of a microtiter plate. Initially the assay performance for the detection of each toxin was examined. We then demonstrated the simultaneous detection of the four toxins from a single sample probed with a mixture of all four QD-antibody reagents. Using a simple linear equation-based algorithm, it was possible to deconvolute the signal from mixed toxin samples, which allowed quantitation of all four toxins simultaneously.
authors.affiliation: Center for Bio/Molecular Science and Engineering and Division of Optical Sciences, U.S. Naval Research Laboratory, Washington, D.C. 20375, USA. erg@cbmse.nrl.navy.mil
authors.forename: Ellen R
authors.initials: ER
authors.lastname: Goldman
authors.forename: Aaron R
authors.initials: AR
authors.lastname: Clapp
authors.forename: George P
authors.initials: GP
authors.lastname: Anderson
authors.forename: H Tetsuo
authors.initials: HT
authors.lastname: Uyeda
authors.forename: J Matthew
authors.initials: JM
authors.lastname: Mauro
authors.forename: Igor L
authors.initials: IL
authors.lastname: Medintz
authors.forename: Hedi
authors.initials: H
authors.lastname: Mattoussi
mesh_terms: D002104:Cadmium / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D005470:Fluorometry / Q000379:methods; D018796:Immunoconjugates / Q000737:chemistry; D007124:Immunoenzyme Techniques; D008163:Luminescent Measurements; D008954:Models, Biological; D015202:Protein Engineering / Q000379:methods; D045663:Quantum Dots; D012643:Selenium / Q000737:chemistry; D012666:Semiconductors; D013440:Sulfides / Q000737:chemistry; D014118:Toxins, Biological / Q000032:analysis* / Q000737:chemistry; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D018796:Immunoconjugates; D013440:Sulfides; D014118:Toxins, Biological; D017967:Zinc Compounds; D002104:Cadmium; D012643:Selenium; C031238:zinc sulfide