title: Qualitative and quantitative analysis of low molecular weight compounds by ultraviolet matrix-assisted laser desorption/ionization mass spectrometry using ionic liquid matrices.
abstract: A major problem hampering the use of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry for quantitative measurements is the inhomogeneous distribution of analytes and matrices in solid sample preparations. The use of ionic liquids as matrices for the qualitative and quantitative analysis of low molecular weight compounds like amino acids, sugars and vitamins was investigated. The ionic liquid matrices are composed of equimolar combinations of classical MALDI matrices (sinapinic acid, alpha-cyano-4-hydroxycinnamic acid or 2,5-dihydroxybenzoic acid) with organic bases. These matrix systems allow a homogenous sample preparation with a thin ionic liquid layer having negligible vapour pressure. This leads to a facilitated qualitative and quantitative measurement of the analytes compared with classical solid matrices.
authors.affiliation: Technische Biochemie, Universität des Saarlandes, 66123 Saarbrücken, Germany.
authors.forename: Masoud
authors.initials: M
authors.lastname: Zabet-Moghaddam
authors.forename: Elmar
authors.initials: E
authors.lastname: Heinzle
authors.forename: Andreas
authors.initials: A
authors.lastname: Tholey
mesh_terms: D000596:Amino Acids / Q000032:analysis / Q000737:chemistry; D002138:Calibration; D007477:Ions / Q000737:chemistry; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry; D012015:Reference Standards; D012996:Solutions / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014466:Ultraviolet Rays
chemical_list: D000596:Amino Acids; D007477:Ions; D009844:Oligosaccharides; D012996:Solutions