Activation of the inflammatory reaction within minutes after birth in ventilated preterm lambs with neonatal respiratory distress syndrome.
To study the activation of the inflammatory reaction within minutes after birth, we measured parameters of inflammation before and immediately after birth. To assess whether respiratory distress syndrome (RDS) or birth itself initiates activation, we compared preterm ventilated lambs with term nonventilated lambs. Preterm lambs were delivered by cesarean section at 132 days gestational age (term 145 days) and were ventilated by conventional ventilation (n = 9). Before clamping the cord, 5, 10 and 15 min after birth, blood was sampled from umbilical catheters. Term lambs (n = 9) were born spontaneously after 140-145 days gestational age. Immediately after birth, a venous umbilical catheter was inserted. Blood was sampled before the first breath and 5, 10, 15 and 20 min after birth while the lamb was breathing spontaneously. Blood was analyzed for AP50 (complement activation), number of polymorphonuclear leukocytes (PMNs) and beta-glucuronidase (released from activated PMNs). In preterm lambs, we found a decreased number of PMNs and increased levels of beta-glucuronidase already at 5 min after birth. In the term lambs, we found only a short-term mild decrease in PMNs and short-term increase in beta-glucuronidase. We conclude that systemic activation of the inflammatory reaction can be found in ventilated preterm lambs with RDS within 5 min after birth. This very early activation is mild, transient and less pronounced in term-born spontaneously breathing lambs compared with preterm, ventilated lambs with RDS.
Jaarsma
Anneke S
AS
Department of Pediatrics, Division of Biocompatibility, University of Groningen, Groningen, The Netherlands. a.s.jaarsma@bkk.azg.nl
Braaksma
Margriethe A
MA
Geven
Wil B
WB
van Oeveren
Wim
W
Bambang Oetomo
Sidarto
S
D000818:Animals; D000831:Animals, Newborn; D003165:Complement System Proteins; D005312:Fetal Blood / Q000737:chemistry / Q000166:cytology; D005865:Gestational Age; D005966:Glucuronidase / Q000097:blood; D007249:Inflammation / Q000097:blood* / Q000150:complications; D007958:Leukocyte Count; D008171:Lung Diseases / Q000097:blood* / Q000150:complications; D009504:Neutrophils; D010100:Oxygen / Q000097:blood; D012121:Respiration, Artificial; D012756:Sheep; D013997:Time Factors
D003165:Complement System Proteins; D005966:Glucuronidase; D010100:Oxygen