On-chip electrochemical measurement of beta-galactosidase expression using a microbial chip.
[small beta]-Galactosidase expression in a small number of Escherichia coli cells has been measured in real time with an electrochemical sensor chip. E. coli cells were embedded using collagen gel within a micropore which was microfabricated onto a chip. The activity of the expressed [small beta]-galactosidase was determined using p-aminophenyl [small beta]-d-galactopyranoside (PAPG) as a substrate.
Kaya
Takatoshi
T
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai, Japan.
Nagamine
Kuniaki
K
Matsui
Nobuto
N
Yasukawa
Tomoyuki
T
Shiku
Hitoshi
H
Matsue
Tomokazu
T
D000627:Aminophenols / Q000737:chemistry; D003094:Collagen; D004563:Electrochemistry / Q000379:methods*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005697:Galactosides / Q000737:chemistry / Q000378:metabolism; D005782:Gels; D017930:Genes, Reporter; D008838:Microcomputers; D011809:Quinones / Q000737:chemistry; D013997:Time Factors; D001616:beta-Galactosidase / Q000032:analysis* / Q000235:genetics / Q000378:metabolism
D000627:Aminophenols; D005697:Galactosides; D005782:Gels; D011809:Quinones; C116897:iminoquinone; C010677:4-aminophenyl-beta-galactoside; D003094:Collagen; D001616:beta-Galactosidase