Two-dimensional protein database of human pancreas.
We report here the two-dimensional protein database of human pancreas. The proteins were analyzed by two-dimensional electrophoresis followed by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS). Totally, 302 proteins were identified, of which about 27% were enzymes with a broad range of catalytic activities. Several of these are specifically expressed in pancreas, such as pancreatic amylase, pancreatic stone protein, pancreatitis-associated protein, pancreatic lipase, pancreatic elastase, etc. Structural and cytoskeletal proteins are also strongly represented on the gels. Thus, the pancreatic proteome reflects the organ's function. This work paves the way for further studies on pancreatic protein expression in health and disease, such as diabetes and pancreatic cancer.
Hu
Liping
L
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, PR China.
Evers
Stefan
S
Lu
Zhao Hui
ZH
Shen
Yan
Y
Chen
Jie
J
D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D010179:Pancreas / Q000737:chemistry*; D000073718:Pancreatitis-Associated Proteins; D011506:Proteins / Q000032:analysis* / Q000145:classification; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000073718:Pancreatitis-Associated Proteins; D011506:Proteins; D020543:Proteome; C000612141:REG3A protein, human