Crystallization of YloQ, a GTPase of unknown function essential for Bacillus subtilis viability.
YloQ is a putative ATP/GTP-binding protein of unknown function identified from the complete sequence of the Bacillus subtilis genome. A gene-knockout programme established that yloQ is one of a set of some 270 indispensable genes for the viability of this organism. Crystals of YloQ have been grown from HEPES-buffered solutions at pH 7.5 containing polyethylene glycol and diffraction data have been collected extending to 2.5 A spacing.
Cladière
Lionel
L
Institut de Génétique et Microbiologie, UMR CNRS 8621, Université Paris-Sud, 91405 Orsay CEDEX, France.
Blagova
Elena
E
Levdikov
Vladimir M
VM
Brannigan
James A
JA
Séror
Simone J
SJ
Wilkinson
Anthony J
AJ
D000255:Adenosine Triphosphate / Q000737:chemistry; D001412:Bacillus subtilis / Q000201:enzymology* / Q000502:physiology*; D001426:Bacterial Proteins / Q000502:physiology*; D002470:Cell Survival; D018360:Crystallography, X-Ray; D020558:GTP Phosphohydrolases / Q000737:chemistry*; D006160:Guanosine Triphosphate / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D019076:Transgenes
D001426:Bacterial Proteins; D011092:Polyethylene Glycols; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D020558:GTP Phosphohydrolases