[A new membrane-permeable peptide from human].
OBJECTIVE
To study the translocation ability of a new protein domain (Circadian locomotor output cycles kaput protein's DNA-binding peptide, hCLOCK's DNA_BIND) from human through the membrane.


METHODS
hCLOCK's DNA_BIND, which was chemically synthesized and labeled with FITC at N-terminal, was incubated with the vascular endothelial cell (ECV-304) and primary culture neuroglial cells in the culture medium, the distribution of it was observed on a fluorescence microscope.


RESULTS
hCLOCK's DNA_BIND has the ability to translocate through the cell membrane and accumulate in the nucleus. The quantification of cellular uptake of it increases with the increase of incubation time and concentration. However, the peptide was internalized at 4 degrees C as efficiently as that at 37 degrees C.


CONCLUSIONS
It is promising to provide a new safe carrier for the intracellular treatment.
Peng
Tao
T
Yang
Chun-lei
CL
Xiao
Jing
J
Guo
Hui-ling
HL
Liu
Ying-hui
YH
Wang
Zheng-rong
ZR
D002462:Cell Membrane / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D005456:Fluorescent Dyes; D006801:Humans; D008856:Microscopy, Fluorescence; D009457:Neuroglia / Q000166:cytology* / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*
D004268:DNA-Binding Proteins; D005456:Fluorescent Dyes; D010455:Peptides