title: Characterization of a lympho-inhibitory peptide produced by Mycoplasma bovis.
abstract: Mycoplasma bovis is able to inhibit the mitogen-induced proliferation of bovine lymphocytes. Herein is described the isolation of an immuno-inhibitory peptide from M. bovis. Using size exclusion chromatography, three lympho-suppressive fractions were isolated from M. bovis free supernatant. MALDI-TOF analysis revealed a common peak throughout the suppressive fractions. The purest of these fractions was subjected to N-terminal sequencing, revealing an 84% homologous match with the C-terminus of the M. bovis surface protein VspL (variable surface protein-L). A recombinant of the 26 amino acid peptide was also able to suppress Concanavalin A (ConA)-induced proliferation of bovine lymphocytes. This describes a unique immunosuppressive peptide produced by the bovine respiratory pathogen, M. bovis.
authors.affiliation: Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
authors.forename: Tony J
authors.initials: TJ
authors.lastname: Vanden Bush
authors.forename: Ricardo F
authors.initials: RF
authors.lastname: Rosenbusch
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry; D015153:Blotting, Western; D002417:Cattle; D002455:Cell Division; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002845:Chromatography; D003208:Concanavalin A / Q000737:chemistry; D005982:Glutathione Transferase / Q000378:metabolism; D007166:Immunosuppressive Agents / Q000737:chemistry; D008214:Lymphocytes / Q000378:metabolism* / Q000382:microbiology*; D008969:Molecular Sequence Data; D045682:Mycoplasma bovis / Q000378:metabolism*; D009555:Ninhydrin / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry; D012825:Silicon / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007166:Immunosuppressive Agents; D010455:Peptides; D011994:Recombinant Proteins; C121437:Vsp protein, bacterial; D003208:Concanavalin A; D005982:Glutathione Transferase; D009555:Ninhydrin; D012825:Silicon