Characterisation of a beta-tubulin gene from the monogenean parasite, Gyrodactylus salaris Malmberg, 1957.
The cDNA and two partial genomic sequences of beta-tubulin genes have been isolated from the monogenean parasite Gyrodactylus salaris. The cDNA sequence is not represented by either of the genomic sequences, implying that at least three isotypes of the gene exist in G. salaris. The sequences show regions of high homology with other helminth beta-tubulin genes. This represents the first isolation of a beta-tubulin gene from a monogenean and contributes to the overall characterisation of these genes within the helminths. This is an important area, as anthelmintic resistance is increasing against benzimidazole drugs that target the beta-tubulin gene. Benzimidazole drugs have been tested successfully against Gyrodactylus parasites, but their use is not widespread. Should it increase, analysis of the beta-tubulin gene may provide a tool for monitoring resistance development and improving management practises. Use of the beta-tubulin gene in the identification of Gyrodactylus species may prove complex due to the presence of different isotypes.
Collins
C M
CM
FRS Marine Laboratory, Victoria Road, PO Box 101, Aberdeen, AB11 9DB, UK. collinsc@marlab.ac.uk
Miller
K A
KA
Cunningham
C O
CO
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018387:Codon, Initiator; D018388:Codon, Terminator / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D018509:DNA, Helminth / Q000737:chemistry / Q000302:isolation & purification; D017238:Genes, Helminth; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012315:RNA Caps / Q000235:genetics; D018510:RNA, Helminth / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014200:Trematoda / Q000235:genetics*; D014404:Tubulin / Q000737:chemistry / Q000235:genetics*
D018387:Codon, Initiator; D018388:Codon, Terminator; D018076:DNA, Complementary; D018509:DNA, Helminth; D012315:RNA Caps; D018510:RNA, Helminth; D012333:RNA, Messenger; D014404:Tubulin