title: Comparative genomic analysis of hyperthermophilic archaeal Fuselloviridae viruses.
abstract: The complete genome sequences of two Sulfolobus spindle-shaped viruses (SSVs) from acidic hot springs in Kamchatka (Russia) and Yellowstone National Park (United States) have been determined. These nonlytic temperate viruses were isolated from hyperthermophilic Sulfolobus hosts, and both viruses share the spindle-shaped morphology characteristic of the Fuselloviridae family. These two genomes, in combination with the previously determined SSV1 genome from Japan and the SSV2 genome from Iceland, have allowed us to carry out a phylogenetic comparison of these geographically distributed hyperthermal viruses. Each virus contains a circular double-stranded DNA genome of approximately 15 kbp with approximately 34 open reading frames (ORFs). These Fusellovirus ORFs show little or no similarity to genes in the public databases. In contrast, 18 ORFs are common to all four isolates and may represent the minimal gene set defining this viral group. In general, ORFs on one half of the genome are colinear and highly conserved, while ORFs on the other half are not. One shared ORF among all four genomes is an integrase of the tyrosine recombinase family. All four viral genomes integrate into their host tRNA genes. The specific tRNA gene used for integration varies, and one genome integrates into multiple loci. Several unique ORFs are found in the genome of each isolate.
authors.affiliation: Thermal Biology Institute and Department of Microbiology, Montana State University, Bozeman, Montana 59717, USA.
authors.forename: Blake
authors.initials: B
authors.lastname: Wiedenheft
authors.forename: Kenneth
authors.initials: K
authors.lastname: Stedman
authors.forename: Francisco
authors.initials: F
authors.lastname: Roberto
authors.forename: Deborah
authors.initials: D
authors.lastname: Willits
authors.forename: Anne-Kathrin
authors.initials: AK
authors.lastname: Gleske
authors.forename: Luisa
authors.initials: L
authors.lastname: Zoeller
authors.forename: Jamie
authors.initials: J
authors.lastname: Snyder
authors.forename: Trevor
authors.initials: T
authors.lastname: Douglas
authors.forename: Mark
authors.initials: M
authors.lastname: Young
mesh_terms: D023641:Archaeal Viruses / Q000235:genetics; D001287:Attachment Sites, Microbiological; D001483:Base Sequence; D005618:Fresh Water / Q000821:virology; D019241:Fuselloviridae / Q000235:genetics*; D016679:Genome, Viral; D023281:Genomics; D006358:Hot Temperature; D008969:Molecular Sequence Data; D012343:RNA, Transfer / Q000235:genetics; D016944:Sulfolobus / Q000821:virology*; D016662:Virus Integration
chemical_list: D012343:RNA, Transfer