title: Heterologous antigen extract in ELISA for the detection of human IgE anti-Strongyloides stercoralis.
abstract: Strongyloides ratti larval extract was used for the standardization of ELISA to detect genus-specific IgE in human strongyloidiasis. Forty serum samples from monoinfected patients shedding S. stercoralis larvae (Group I), 40 from patients with other intestinal parasites (Group II), and 40 from copronegative healthy subjects (Group III) were analyzed. Genus-specific IgE levels (ELISA Index: EI) were significantly higher in the group I (EI = 1.43) than groups II (EI = 0.70) and III (EI = 0.71), showing positivity rates of 55%, 2.5% and 0%, respectively. Similarly, sera from copropositive patients had significantly higher levels of total IgE (866 IU/mL) as compared to those from group II (302 IU/mL) and III (143 IU/mL). A significant positive correlation was found between levels of Strongyloides specific-IgE and total IgE in sera from patients with strongyloidiasis. In conclusion, S. ratti heterologous extract showed to be a useful tool for detecting genus-specific IgE by ELISA, contributing for a better characterization of the immune response profile in human strongyloidiasis.
authors.affiliation: Area de Imunologia, Microbiologia e Parasitologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brasil.
authors.forename: Julia Maria
authors.initials: JM
authors.lastname: Costa-Cruz
authors.forename: Joaquina
authors.initials: J
authors.lastname: Madalena
authors.forename: Deise Aparecida de Oliveira
authors.initials: DA
authors.lastname: Silva
authors.forename: Mônica Camargo
authors.initials: MC
authors.lastname: Sopelete
authors.forename: Dulcinéa Maria Barbosa
authors.initials: DM
authors.lastname: Campos
authors.forename: Ernesto Akio
authors.initials: EA
authors.lastname: Taketomi
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D015478:Antigens, Heterophile / Q000276:immunology* / Q000302:isolation & purification; D015415:Biomarkers / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D008297:Male; D012680:Sensitivity and Specificity; D017170:Strongyloides ratti / Q000276:immunology*; D013322:Strongyloidiasis / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015478:Antigens, Heterophile; D015415:Biomarkers; D007073:Immunoglobulin E