Growth responses of Escherichia coli to immunoglobulin G from cows immunized with ferric citrate receptor, FecA.
Effects of purified immunoglobulin (Ig) G from cows immunized with ferric citrate receptor, FecA, on the in vitro growth of Escherichia coli were investigated. Twenty-one cows were assigned to one of 3 treatments: 1) FecA immunization, 2) E. coli J5 bacterin immunization, and 3) unimmunized control. FecA was derived from E. coli UT5600/pSV66. Immunoglobulin G was purified from pooled colostral whey for each treatment group. The IgG from FecA immunized cows had higher titers against FecA compared with other treatment groups. Bacterial isolates tested were 14 E. coli from intramammary infections and E. coli UT5600/pSV66. Iron depletion decreased the growth of E. coli compared with growth in Fe-replete medium. The presence of IgG further decreased the growth compared with the growth under iron restriction alone. Bacterial growth did not differ among IgG sources nor between IgG concentrations. Replenishing media with exogenous iron overrode the inhibitory effects of the Fe-depletion and IgG. Vaccinating cows with FecA had little effect on the growth inhibitory properties of IgG toward E. coli mastitis isolates cultured in Fe-deplete media.
Takemura
K
K
Department of Animal Sciences, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster 44691, USA.
Hogan
J S
JS
Smith
K L
KL
D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology; D002352:Carrier Proteins / Q000276:immunology*; D002417:Cattle / Q000276:immunology; D004926:Escherichia coli / Q000254:growth & development*; D029968:Escherichia coli Proteins; D005260:Female; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology*; D008414:Mastitis, Bovine / Q000382:microbiology*; D011956:Receptors, Cell Surface
D001428:Bacterial Vaccines; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C028589:FecA protein, E coli; D007074:Immunoglobulin G; D011956:Receptors, Cell Surface