Naringenin from Citrus junos has an inhibitory effect on acetylcholinesterase and a mitigating effect on amnesia.
This study was performed to identify safe and more effective acetylcholinesterase (AChE) inhibitors in the treatment of Alzheimer's disease. The total methanol extract of Citrus junos had a significant inhibitory effect on AChE in vitro. By sequential fractionation of C.junos, the active component was finally identified as naringenin. Naringenin inhibited AChE activity in a dose-dependent manner. In this study, we also evaluated the anti-amnesic activity of naringenin, a major flavanone constituent isolated from C. junos, in vivo using ICR mice with amnesia induced by scopolamine (1 mg/kg body weight). Naringenin, when administered to mice at 4.5 mg/kg body weight, significantly ameliorated scopolamine-induced amnesia as measured in both the passive avoidance and the Y-maze test. These results suggest that naringenin may be a useful chemopreventive agent against Alzheimer's disease.
Heo
Ho Jin
HJ
Graduate School of Biotechnology, Korea University, Seoul, Korea.
Kim
Mi-Jeong
MJ
Lee
Jung-Min
JM
Choi
Soo Jung
SJ
Cho
Hong-Yon
HY
Hong
Bumshik
B
Kim
Hye-Kyung
HK
Kim
Eunki
E
Shin
Dong-Hoon
DH
D000110:Acetylcholinesterase / Q000032:analysis; D000647:Amnesia / Q000139:chemically induced / Q000188:drug therapy*; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D001522:Behavior, Animal / Q000187:drug effects; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D002855:Chromatography, Thin Layer; D002957:Citrus / Q000737:chemistry*; D044950:Flavanones / Q000494:pharmacology* / Q000627:therapeutic use*; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D000432:Methanol; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D012601:Scopolamine; D012997:Solvents
D002800:Cholinesterase Inhibitors; D044950:Flavanones; D010936:Plant Extracts; D012997:Solvents; D012601:Scopolamine; D000110:Acetylcholinesterase; C005273:naringenin; D000432:Methanol