title: Photodynamic effects of two water soluble porphyrins evaluated on human malignant melanoma cells in vitro.
abstract: Two water soluble porphyrins: meso-tetra-4-N-methylpyridyl-porphyrin iodide (P1) and 5,10-di-(4-acetamidophenyl)-15,20-di-(4-N-methylpyridyl) porphyrin (P2) were synthesised and evaluated in respect to their photochemical and photophysical properties as well as biological activity. Cytotoxic and phototoxic effects were evaluated in human malignant melanoma Me45 line using clonogenic assay, cytological study of micronuclei, apoptosis and necrosis frequency and inhibition of growth of megacolonies. Both porphyrins were characterised by high UV and low visible light absorptions. Dark toxicity measured on the basis of the clonogenic assay and inhibition of megacolony growth area indicated that P1 was non-toxic at concentrations up to 50 microg/ml (42.14 microM) and P2 at concentrations up to 20 microg/ml (16.86 microM). The photodynamic effect induced by red light above 630 nm indicated that both porphyrins were able to inhibit growth of melanoma megacolonies at non-toxic concentrations. Cytologic examination showed that the predominant mode of cell death was necrosis.
authors.affiliation: Institute of Physics, University of Silesia, Katowice, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Szurko
authors.forename: Gabriela
authors.initials: G
authors.lastname: Krämer-Marek
authors.forename: Maria
authors.initials: M
authors.lastname: Wideł
authors.forename: Alicja
authors.initials: A
authors.lastname: Ratuszna
authors.forename: Jan
authors.initials: J
authors.lastname: Habdas
authors.forename: Piotr
authors.initials: P
authors.lastname: Kuś
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D017209:Apoptosis / Q000502:physiology; D006801:Humans; D008545:Melanoma / Q000188:drug therapy / Q000378:metabolism*; D048629:Micronuclei, Chromosome-Defective / Q000378:metabolism; D009336:Necrosis; D010778:Photochemotherapy; D011166:Porphyrins / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D013053:Spectrophotometry
chemical_list: D000970:Antineoplastic Agents; D011166:Porphyrins