Gastric mucosal cytokine and epithelial cell responses to Helicobacter pylori infection in Mongolian gerbils.
Experimental infection with Helicobacter pylori in Mongolian gerbils results in chronic gastritis and gastric cancer. To investigate epithelial cell proliferation, apoptosis, and mucosal cytokine responses in gastritis, Mongolian gerbils were infected with the H pylori SS1 strain. At 4 weeks post-infection, gastritis was predominantly within the antrum, but extended to the corpus in approximately 50% of gerbils by 36 weeks. Epithelial cell proliferation and apoptosis in glandular epithelial cells were increased with infection. Antral cell proliferation, but not apoptosis, correlated significantly with gastric inflammation. In female gerbils, H pylori significantly increased expression of transcripts for IFN-gamma and IL-12p40, but not TGF-beta or IL-10, in the gastric mucosa. Significantly reduced IFN-gamma and IL-12p40 responses were observed in male gerbils infected with H pylori, but epithelial proliferative and apoptotic responses were comparable to those of females. These studies demonstrate that the female gerbil cytokine response to H pylori has a Th1 profile and that there are gender differences in the magnitude of the gastric cytokine responses to H pylori. The absence of a down-regulatory cytokine response may account for the more severe gastritis observed with H pylori infection in gerbils than in mice.
Crabtree
Jean E
JE
Molecular Medicine Unit, St James's University Hospital, Leeds, UK. j.crabtree@leeds.ac.uk
Court
Michelle
M
Aboshkiwa
Mohamed A
MA
Jeremy
Anthony H T
AH
Dixon
Michael F
MF
Robinson
Philip A
PA
D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D002455:Cell Division; D006224:Cricetinae; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D018450:Disease Progression; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D005753:Gastric Mucosa / Q000276:immunology* / Q000473:pathology; D005756:Gastritis / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D015870:Gene Expression; D005849:Gerbillinae; D016481:Helicobacter Infections / Q000276:immunology* / Q000473:pathology; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity
D016207:Cytokines