Heterologous antigen extract in ELISA for the detection of human IgE anti-Strongyloides stercoralis.
Strongyloides ratti larval extract was used for the standardization of ELISA to detect genus-specific IgE in human strongyloidiasis. Forty serum samples from monoinfected patients shedding S. stercoralis larvae (Group I), 40 from patients with other intestinal parasites (Group II), and 40 from copronegative healthy subjects (Group III) were analyzed. Genus-specific IgE levels (ELISA Index: EI) were significantly higher in the group I (EI = 1.43) than groups II (EI = 0.70) and III (EI = 0.71), showing positivity rates of 55%, 2.5% and 0%, respectively. Similarly, sera from copropositive patients had significantly higher levels of total IgE (866 IU/mL) as compared to those from group II (302 IU/mL) and III (143 IU/mL). A significant positive correlation was found between levels of Strongyloides specific-IgE and total IgE in sera from patients with strongyloidiasis. In conclusion, S. ratti heterologous extract showed to be a useful tool for detecting genus-specific IgE by ELISA, contributing for a better characterization of the immune response profile in human strongyloidiasis.
Costa-Cruz
Julia Maria
JM
Area de Imunologia, Microbiologia e Parasitologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brasil.
Madalena
Joaquina
J
Silva
Deise Aparecida de Oliveira
DA
Sopelete
Mônica Camargo
MC
Campos
Dulcinéa Maria Barbosa
DM
Taketomi
Ernesto Akio
EA
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D015478:Antigens, Heterophile / Q000276:immunology* / Q000302:isolation & purification; D015415:Biomarkers / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D008297:Male; D012680:Sensitivity and Specificity; D017170:Strongyloides ratti / Q000276:immunology*; D013322:Strongyloidiasis / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015478:Antigens, Heterophile; D015415:Biomarkers; D007073:Immunoglobulin E