title: Total antioxidant capacity of arginine-conjugated linoleic acid (CLA) complex.
abstract: Conjugated linoleic acids (CLA) have shown diverse biological activities, including anti-carcinogenic, anti-atherosclerotic, anti-adipogenic, and anti-diabetogenic effects. Recent reports also showed that CLA has free radical scavenging capacity, which may give health benefits to human beings. However, the application of CLA as a bioactive ingredient has been limited due to its insolubility in water. To overcome this problem, we investigated antioxidant activities of arginine (Arg)-CLA, a water-soluble CLA salt, using both 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. CLA, Arg, and Arg-CLA all exerted radical scavenging activities in a dose-dependent manner in both assays. Arg-CLA at 20 mM scavenged 89 and 55% of ABTS and DPPH radicals in 3 h, respectively, whereas CLA alone quenched only 48 and 26% of them under the same conditions. The antioxidant activity of the Arg-CLA complex was found to be synergistic in ABTS assay and comparable to that of vitamin E in DPPH assay.
authors.affiliation: Department of Molecular Biology and Genetics, Cornell University, New York, Ithaca 14853, USA.
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Kim
authors.forename: Ki Won
authors.initials: KW
authors.lastname: Lee
authors.forename: Hyong Joo
authors.initials: HJ
authors.lastname: Lee
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001120:Arginine / Q000031:analogs & derivatives* / Q000494:pharmacology; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D044243:Linoleic Acids, Conjugated / Q000737:chemistry / Q000494:pharmacology*; D010851:Picrates; D012995:Solubility; D013451:Sulfonic Acids; D014867:Water
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D044243:Linoleic Acids, Conjugated; D010851:Picrates; D013451:Sulfonic Acids; D014867:Water; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D001120:Arginine; C004931:1,1-diphenyl-2-picrylhydrazyl