title: Functional and structural characterization of a novel member of the natriuretic family of peptides from the venom of Pseudocerastes persicus.
abstract: A novel peptide, PNP (Pseudocerastes persicus natriuretic peptide), was isolated from the venom of the Iranian viper P. persicus. Amino acid sequencing revealed that the 37-residue peptide belongs to the family of natriuretic peptides. The physiological effects of intra-venously PNP infused into anesthetized rats on urine flow, sodium excretion and blood pressure were comparable to those of atrial natriuretic peptide (ANP). In PC12 cells that were treated with either PNP, ANP, or C-type natriuretic peptide, PNP induced a similar cGMP response as ANP. Since PC12 cells only express the natriuretic peptide receptor (NPR)-A receptor we conclude that PNP binds to the NPR-A receptor. The solution conformation of PNP was characterized using (1)H nuclear magnetic resonance spectroscopy and indicates a high degree of conformational flexibility.
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
authors.forename: Mehriar
authors.initials: M
authors.lastname: Amininasab
authors.forename: Maryam M
authors.initials: MM
authors.lastname: Elmi
authors.forename: Nicole
authors.initials: N
authors.lastname: Endlich
authors.forename: Karlhans
authors.initials: K
authors.lastname: Endlich
authors.forename: Niranjah
authors.initials: N
authors.lastname: Parekh
authors.forename: Hossein
authors.initials: H
authors.lastname: Naderi-Manesh
authors.forename: Johann
authors.initials: J
authors.lastname: Schaller
authors.forename: Hossein
authors.initials: H
authors.lastname: Mostafavi
authors.forename: Michael
authors.initials: M
authors.lastname: Sattler
authors.forename: Mohammad N
authors.initials: MN
authors.lastname: Sarbolouki
authors.forename: Claudia
authors.initials: C
authors.lastname: Muhle-Goll
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D017124:Conserved Sequence; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D045265:Natriuretic Peptides / Q000737:chemistry* / Q000494:pharmacology; D011487:Protein Conformation; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014757:Viper Venoms / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D017819:Viperidae
chemical_list: D045265:Natriuretic Peptides; D014757:Viper Venoms