title: Secreted brevican mRNA is expressed in the adult rat pituitary.
abstract: Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix (ECM) of the adult rat brain. It has been found only in the central nervous system (CNS). In this study, we found that secreted brevican transcript was detectable in the pituitary of both male and female adult rats by reverse transcriptase-polymerase chain reaction (RT-PCR) amplification. In posterior lobe of pituitary, pituicytes were heavily labelled. In anterior and intermediate lobes of pituitary, signals for brevican transcripts were observed in cells of various sizes. These data demonstrated that secreted brevican mRNA is expressed in the adult rat pituitary and brevican might not be a CNS-specific ECM.
authors.affiliation: Department of Neurosurgery, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, 200003, Shanghai, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Dong
authors.forename: Xi
authors.initials: X
authors.lastname: Han
authors.forename: Yajun
authors.initials: Y
authors.lastname: Xue
authors.forename: Bin
authors.initials: B
authors.lastname: Dong
authors.forename: Xingzhong
authors.initials: X
authors.lastname: Guo
authors.forename: Guohan
authors.initials: G
authors.lastname: Hu
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhu
authors.forename: Yicheng
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000818:Animals; D058581:Brevican; D011508:Chondroitin Sulfate Proteoglycans / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization; D037181:Lectins, C-Type; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*; D010902:Pituitary Gland / Q000378:metabolism*; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C546938:BCAN protein, human; C546939:Bcan protein, rat; D058581:Brevican; D011508:Chondroitin Sulfate Proteoglycans; D037181:Lectins, C-Type; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger