Adiponectin gene expression and plasma values in obese women during very-low-calorie diet. Relationship with cardiovascular risk factors and insulin resistance.
Adiponectin, a newly discovered adipose-tissue-specific protein, is thought to be involved in the regulation of insulin action. The aim of the present study was to determine whether adiponectin contributes to the improvement in insulin sensitivity during very-low-calorie diet (VLCD). Biopsies of sc abdominal adipose tissue and blood sampling for analysis of plasma adiponectin and related hormones and metabolites were performed before and at the end of a 4-wk VLCD in 33 nonmorbidly obese women (body mass index, 34.4 +/- 4.1 kg/m(2)). VLCD produced a decrease in weight (7.1 +/- 0.4 kg) and in insulin and leptin levels and led to an improvement in insulin sensitivity. Adiponectin gene expression and plasma levels were not modified during calorie restriction. Before VLCD, we found negative correlations between plasma adiponectin and variables related to the metabolic syndrome. Adiponectin mRNA levels showed a negative correlation with lipoprotein a plasma values. The correlations observed before VLCD were not found after VLCD. The data suggest that adiponectin is related to the protection against the metabolic syndrome but is not involved in the regulation of VLCD-induced improvement of insulin sensitivity.
Garaulet
Marta
M
Franco-Czech Laboratory for Clinical Research on Obesity, French Institute of Health and Medical Research, Institut National de la Santé et d la Recherche Médicale U586 and 3rd Faculty of Medicine, Charles University, Prague, Czech Republic.
Viguerie
Nathalie
N
Porubsky
Stefan
S
Klimcakova
Eva
E
Clement
Karine
K
Langin
Dominique
D
Stich
Vladimir
V
D000005:Abdomen; D052242:Adiponectin; D000273:Adipose Tissue / Q000378:metabolism / Q000473:pathology; D000328:Adult; D002318:Cardiovascular Diseases / Q000209:etiology; D004038:Diet, Reducing; D002149:Energy Intake; D005260:Female; D005269:Femur; D015870:Gene Expression; D006801:Humans; D007333:Insulin Resistance; D036341:Intercellular Signaling Peptides and Proteins; D024821:Metabolic Syndrome / Q000503:physiopathology; D009765:Obesity / Q000178:diet therapy* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012307:Risk Factors; D040521:Subcutaneous Tissue / Q000378:metabolism
D052242:Adiponectin; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins; D012333:RNA, Messenger