title: The potential for gamete recovery from non-domestic canids and felids.
abstract: Species are becoming extinct at a rate 100 times the natural background rates. Considering all mammalian orders, 24% of all Carnivora species are threatened. The goal of carnivore conservation is to reverse the decline in populations and to secure remaining populations in ways that will assure enduring public support. In this context, biotechnology is a tool with tremendous potential for assisting the conservation of endangered canid and felid species. As the first step for biotechnology development is the gamete obtainment, this review will discuss the potential of gamete recovery from non-domestic canids and felids, based on learning how to apply these procedures in the domestic carnivores. Thus, electroejaculation and obtaining both epidydimal spermatozoon and spermatogonial germ cells are indicated as techniques for male gametes recovery. In the female gametes retrieval, different methods for oocyte recovery from both antral and preantral follicles, and the possibility for ovarian tissue transplantation are discussed. Furthermore, the study discusses the responsibilities involved in the use of assisted reproduction in endangered species conservation.
authors.affiliation: Laboratory of Carnivore Reproduction, PPGCV-UECE, Paranjana Ave. 1700, Itaperi, Fortaleza, Ceará 60740-000, Brazil. legio2000@yahoo.com
authors.forename: Alexandre R
authors.initials: AR
authors.lastname: Silva
authors.forename: Ronaldo G
authors.initials: RG
authors.lastname: Morato
authors.forename: Lúcia D M
authors.initials: LD
authors.lastname: Silva
mesh_terms: D000818:Animals; D002335:Carnivora; D003247:Conservation of Natural Resources; D004542:Ejaculation; D004822:Epididymis / Q000166:cytology; D005260:Female; D008297:Male; D009865:Oocytes; D006080:Ovarian Follicle / Q000166:cytology; D010053:Ovary / Q000166:cytology; D013093:Spermatogonia; D013094:Spermatozoa; D020858:Tissue and Organ Harvesting / Q000379:methods / Q000662:veterinary*