title: Pseudonocardia chloroethenivorans sp. nov., a chloroethene-degrading actinomycete.
abstract: A bacterial strain, SL-1T, capable of degrading trichloroethene was isolated from a laboratory enrichment in the Department of Civil and Environmental Engineering, University of Washington, USA. The material in the enrichments was derived from a soil sample from Seattle, WA, USA. Strain SL-1T was capable of using phenol as a source of carbon and energy. Chemotaxonomic, morphological, physiological and phylogenetic analyses showed that strain SL-1T is a member of the genus Pseudonocardia. The ability of strain SL-1T to utilize phenol and degrade trichloroethene, as well as other phenotypic properties and the results from a 16S rRNA phylogenetic analysis, led to the proposal of a novel species, Pseudonocardia chloroethenivorans sp. nov. The type strain is SL-1T (=ATCC BAA-742T=DSM 44698T). Trichloroethene and other chloroethenes are major pollutants at many environmental sites, and P. chloroethenivorans has biodegradation properties that should be of interest to environmental microbiologists and engineers.
authors.affiliation: Department of Civil and Environmental Engineering, Box 352700, University of Washington, Seattle, WA 98105-2700, USA.
authors.forename: Seung-Bong
authors.initials: SB
authors.lastname: Lee
authors.affiliation: College of Forest Resources, Box 352100, University of Washington, Seattle, WA 98105-2100, USA.
authors.forename: Stuart E
authors.initials: SE
authors.lastname: Strand
authors.affiliation: Department of Civil and Environmental Engineering, Box 352700, University of Washington, Seattle, WA 98105-2700, USA.
authors.forename: H David
authors.initials: HD
authors.lastname: Stensel
authors.affiliation: School of Aquatic and Fishery Sciences, Box 355020, University of Washington, Seattle, WA 98195-5020, USA.
authors.forename: Russell P
authors.initials: RP
authors.lastname: Herwig
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D001673:Biodegradation, Environmental; D018533:Biomass; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004785:Environmental Pollutants; D008969:Molecular Sequence Data; D019800:Phenol / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D014752:Vinyl Chloride / Q000493:pharmacokinetics*; D014861:Washington
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004785:Environmental Pollutants; D012336:RNA, Ribosomal, 16S; D019800:Phenol; D014752:Vinyl Chloride