title: Hepatitis B virus X protein modulates peroxisome proliferator-activated receptor gamma through protein-protein interaction.
abstract: Ligand activation of peroxisome proliferator-activated receptor gamma (PPARgamma) has been reported to induce growth inhibition and apoptosis in various cancers including hepatocellular carcinoma (HCC). However, the effect of hepatitis B virus X protein (HBx) on PPARgamma activation has not been characterized in hepatitis B virus (HBV)-associated HCC. Herein, we demonstrated that HBx counteracted growth inhibition caused by PPARgamma ligand in HBx-associated HCC cells. We found that HBx bound to DNA binding domain of PPARgamma and HBx/PPARgamma interaction blocked nuclear localization and binding to recognition site of PPARgamma. HBx significantly suppressed a PPARgamma-mediated transactivation. These results suggest that HBx modulates PPARgamma function through protein-protein interaction.
authors.affiliation: Department of Microbiology and Brain Korea 21 Project of Medical Sciences, Institute for Immunology and Immunological Diseases, Seoul, South Korea.
authors.forename: Youn-Hee
authors.initials: YH
authors.lastname: Choi
authors.forename: Ha-il
authors.initials: HI
authors.lastname: Kim
authors.forename: Je Kyung
authors.initials: JK
authors.lastname: Seong
authors.forename: Dae-Yeul
authors.initials: DY
authors.lastname: Yu
authors.forename: Hyeseong
authors.initials: H
authors.lastname: Cho
authors.forename: Mi-Ock
authors.initials: MO
authors.lastname: Lee
authors.forename: Jae Myun
authors.initials: JM
authors.lastname: Lee
authors.forename: Yong-ho
authors.initials: YH
authors.lastname: Ahn
authors.forename: Se Jong
authors.initials: SJ
authors.lastname: Kim
authors.forename: Jeon Han
authors.initials: JH
authors.lastname: Park
mesh_terms: D000256:Adenoviridae; D017209:Apoptosis / Q000187:drug effects; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000821:virology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D017931:DNA Primers; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D006511:Hepatitis B Antigens / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008024:Ligands; D008113:Liver Neoplasms / Q000473:pathology / Q000821:virology; D008164:Luminescent Proteins / Q000235:genetics; D021381:Protein Transport / Q000187:drug effects; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D014157:Transcription Factors / Q000737:chemistry / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D017931:DNA Primers; D006511:Hepatitis B Antigens; D008024:Ligands; D008164:Luminescent Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; D049452:Green Fluorescent Proteins