Determination of corticosterone and 17-hydroxycorticosterone in plasma and urine samples by sweeping techniques using micellar electrokinetic chromatography.
The analysis of corticosterone in mouse blood serum (metabolic-stress experiment) and 17-hydroxycorticosterone in human urine (exercise-stress experiment) samples by means of capillary electrophoresis/UV absorbance in conjunction with online sample concentration techniques is described. The use of normal MEKC had an analyte detection limit of 7 microg/ml (S/N=3); whereas when online sample concentration methods, including sweeping-micellar electrokinetic chromatography (Sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography (CSEI-sweep-MEKC) were used, the detection limits could be improved to 3 and 5 ng/ml, respectively. In the analysis of actual samples from animal metabolic-stress experiments (39 mouse), chronically stressed animals showed a higher level (552+/-152 ng/ml) and acute stressed animals showed an intermediate level (375+/-105 ng/ml). In comparison, normal animals show a lower concentration level of corticosterone (153+/-109 ng/ml). In addition, based on a human exercise-stress experiment (seven volunteers), the acute stressed humans (after exercise, 800 m of running) show a higher concentration of 17-hydroxycorticosterone (113+/-55 ng/ml for males; 128+/-25 for females) and the non-stressed humans (before exercise) show a lower concentration (63+/-37 ng/ml for male; 60+/-20 for female), respectively.
Chen
Mei-Chuan
MC
Department of Chemistry, National Taiwan Normal University, 88 Sec. 4 Tingchow Road, 116 Taipei, Taiwan.
Chou
Shiu-Huey
SH
Lin
Cheng-Huang
CH
D015069:18-Hydroxycorticosterone / Q000097:blood* / Q000652:urine*; D000818:Animals; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003345:Corticosterone / Q000097:blood* / Q000652:urine*; D015444:Exercise; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D012680:Sensitivity and Specificity; D013312:Stress, Physiological / Q000097:blood / Q000652:urine
D015069:18-Hydroxycorticosterone; D003345:Corticosterone