title: Retention of Cr(III) by high-performance chelation ion chromatography interfaced to inductively-coupled plasma mass spectrometric detection with collision cell.
abstract: High-performance chelation ion chromatography (HPCIC) was employed to retain cationic Cr(III) on an anion-exchange column and hence allow the separation of the two most prevalent forms of chromium, Cr(II) and Cr(VI). A mobile phase of nitric acid was utilized at pH = 1.5; additionally, 2,6-pyridinedicarboxylic acid was used at a concentration of 6 mM. Additives with different structural characteristics were used in an effort to elucidate retention mechanisms. Inductively-coupled plasma mass spectrometry was used for chromium detection. A collision cell was utilized to reduce chloride-based polyatomic ions that may interfere with the detection of Cr(III), and a detection limit study yielded levels in the low part-per-billion range. The newly developed method was applied to the chromatographic analysis of samples of an incubation medium containing Cr(VI) incubated with cell nuclei.
authors.affiliation: Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221-0172, USA.
authors.forename: Anne P
authors.initials: AP
authors.lastname: Vonderheide
authors.forename: Juris
authors.initials: J
authors.lastname: Meija
authors.forename: Katherine
authors.initials: K
authors.lastname: Tepperman
authors.forename: Alvaro
authors.initials: A
authors.lastname: Puga
authors.forename: Allan R
authors.initials: AR
authors.lastname: Pinhas
authors.forename: J Christopher
authors.initials: JC
authors.lastname: States
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Caruso
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002614:Chelating Agents / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002857:Chromium / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D012680:Sensitivity and Specificity
chemical_list: D002614:Chelating Agents; D002857:Chromium