High levels of IgG class antibodies to recombinant HSP60 kDa of Yersinia enterocolitica in sera of patients with uveitis.
OBJECTIVE
To determine the levels of IgG class antibodies to recombinant heat shock protein 60 kDa of Yersinia enterocolitica (rHSP60Ye), Klebsiella pneumoniae (rHSP60Kp), Escherichia coli (rHSP60Ec), Shigella flexneri (rHSP60Sf), and Streptococcus pyogenes (rHSP60Sp) in the serum of patients with HLA-B27 associated acute anterior uveitis (HLA-B27 associated AAU), idiopathic acute anterior uveitis (idiopathic AAU), pars planitis, Vogt-Koyanagi-Harada (VKH), and healthy subjects.


METHODS
The genes that code for HSP60Ye, HSP60Kp, HSP60Ec, HSP60Sf, and HSP60Sp were cloned by PCR from genomic DNA. The rHSPs were purified by affinity using a Ni-NTA resin. The serum levels of IgG class antibodies to rHSP60s were determined by ELISA in patients with uveitis (n = 42) and in healthy subjects (n = 25).


RESULTS
The majority of patients with uveitis had higher levels of IgG class antibodies to rHSP60Ye compared with levels of healthy subjects (p = 0.01), although these differences were only observed in the HLA-B27 associated AAU (p = 0.005) and in pars planitis patients (p = 0.001). The levels of IgG antibodies to the rHSP60Kp, rHSP60Sf, rHSP60Ec, and rHSP60Sp were similar in patients with uveitis and in healthy subjects (p>0.05).


CONCLUSIONS
The results suggest that HSP60Ye could be involved in the aetiology of HLA-B27 associated AAU and pars planitis.
Cancino-Diaz
J C
JC
Laboratorio de Inmunología ocular del Instituto de Oftalmología Fundación Conde de Valenciana, Mexico. mcancino@encb.ipn.mx
Vargas-Rodríguez
L
L
Grinberg-Zylberbaum
N
N
Reyes-López
M A
MA
Domínguez-López
M L
ML
Pablo-Velazquez
A
A
Cancino-Diaz
M E
ME
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D018834:Chaperonin 60 / Q000276:immunology*; D005260:Female; D020022:Genetic Predisposition to Disease; D015796:HLA-B27 Antigen / Q000097:blood; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D015868:Pars Planitis / Q000276:immunology / Q000382:microbiology*; D011994:Recombinant Proteins / Q000276:immunology; D012008:Recurrence; D014606:Uveitis, Anterior / Q000276:immunology / Q000382:microbiology*; D014607:Uveomeningoencephalitic Syndrome / Q000276:immunology / Q000382:microbiology; D015008:Yersinia enterocolitica / Q000276:immunology*
D000907:Antibodies, Bacterial; D018834:Chaperonin 60; D015796:HLA-B27 Antigen; D007074:Immunoglobulin G; D011994:Recombinant Proteins