Proteomic evaluation of core biopsy specimens from breast lesions.
Analysis of tumour samples by a proteomic technology, which combines two-dimensional gel electrophoresis and mass spectrometry analysis, is a promising approach for molecular characterization of cancer. Proteomic analysis of neoplasms is usually performed on surgical material. The possibility to perform proteomic analysis on pre-operative samples might be useful for diagnostic purposes or for determination of tumour sensitivity to therapy. In this study, we report how tissues from core biopsy of breast lesions can be routinely used to obtain accurate protein expression profiles by proteomic analysis. Protein profiles from fibroadenomas were compared to those from ductal infiltrating carcinomas. By using mass spectrometry, identification of proteins overexpressed in carcinomas with respect to fibroadenomas was obtained. Thus, our study provides a methodology to perform proteomic analysis on pre-operative samples of breast lesions.
Bisca
Alessia
A
Dipartimento di Scienze e Tecnologie Biomediche, Centro M.A.T.I., Università di Udine, Piazzale Kolbe 1, Udine 33100, Italy.
D'Ambrosio
Chiara
C
Scaloni
Andrea
A
Puglisi
Fabio
F
Aprile
Giuseppe
G
Piga
Andrea
A
Zuiani
Chiara
C
Bazzocchi
Massimo
M
Di Loreto
Carla
C
Paron
Igor
I
Tell
Gianluca
G
Damante
Giuseppe
G
D014408:Biomarkers, Tumor / Q000032:analysis*; D001706:Biopsy; D001943:Breast Neoplasms / Q000737:chemistry* / Q000175:diagnosis; D018270:Carcinoma, Ductal, Breast / Q000737:chemistry* / Q000175:diagnosis; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D018226:Fibroadenoma / Q000737:chemistry* / Q000175:diagnosis; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis*; D010446:Peptide Fragments / Q000737:chemistry; D010449:Peptide Mapping; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D010446:Peptide Fragments