Relationship between neuropeptide Y and luteinizing-hormone-releasing hormone immunoreactivities in the hypothalamus and preoptic region.
The purpose of this study was to examine the anatomical relationships of perikarya and fibers containing neuropeptide Y (NPY) and luteinizing-hormone-releasing hormone (LHRH) in the hypothalamus and preoptic region of female rats. In view of our previous report of stimulatory effects of estrogen on LHRH and NPY levels in the median eminence, animals were bilaterally ovariectomized and subsequently implanted subcutaneously with capsules containing estradiol benzoate in oil or vehicle. Following intracerebroventricular injection of colchicine, rats were perfused with fixative and their brains sectioned and processed for immunohistochemical visualization of NPY and LHRH in the same section and in consecutive sections. Estrogen treatment had no discernible effect on the distribution or relationship of these peptides. NPY-immunoreactive fibers were intimately associated with LHRH-labeled primary dendrites and perikarya in the medial preoptic region and horizontal limb of the diagonal band of Broca. Fibers containing NPY or LHRH overlapped extensively in the lateral palisade region of the median eminence and also in the subependymal and internal zones. The external zone of the median eminence displayed relatively less overlap of these peptide systems. LHRH-immunoreactive axons coursed among NPY-labeled perikarya in the arcuate nucleus and appeared to contact these cells. These results suggest that NPY-containing axons may influence LHRH-positive neurons at the cell body and also at the site of axon termination in the median eminence. LHRH-containing axons appear to contact NPY-immunoreactive perikarya in the arcuate nucleus and may interact with terminals in the median eminence. This arrangement may provide a mechanism for communication between NPY and LHRH neurons and for the neuroendocrine coordination of hypothalamic NPY and LHRH secretion before ovulation.
McDonald
J
J
Department of Anatomy and Cell Biology, Emory University School of Medicine, Atlanta, Ga 30322, USA.
Calka
J
J
D000818:Animals; D000906:Antibodies; D001111:Arcuate Nucleus of Hypothalamus / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D002450:Cell Communication; D020667:Diagonal Band of Broca / Q000737:chemistry / Q000166:cytology / Q000502:physiology; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D007150:Immunohistochemistry; D008473:Median Eminence / Q000737:chemistry / Q000166:cytology / Q000502:physiology; D009412:Nerve Fibers / Q000737:chemistry; D009478:Neuropeptide Y / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D011301:Preoptic Area / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000906:Antibodies; D009478:Neuropeptide Y; D007987:Gonadotropin-Releasing Hormone