Flow-through spectrophotometric sensor for the determination of saccharin in low-calorie products.
A simple, rapid and inexpensive monoparameter flow-through sensor has been developed for the determination of saccharin in low calorie and dietary products. The method is based on the transient adsorption of the sweetener on Sephadex G-25 solid phase packed to a height of 20 mm in the flow cell. The optimal transient retention of the synthetic sweetener, in terms of sensitivity and sampling frequency, was obtained when pH 2.75 citric acid-sodium citrate buffer 5 x 10(-3) M was used as a carrier at a flow-rate of 1.5 ml min(-1). Saccharin was determined measuring its intrinsic absorbance at 217 nm at its residence time. Calibration graphs for peak height and peak area were linear over the range 5.0-200.0 microg ml(-1), RSD 1.18%, and 1.0-200.0 microg ml(-1), RSD 0.78%, respectively. Saccharin was determined in several food samples measuring height or area peak, obtaining recoveries ranging between 98-104 and 99-102% for height and area peak, respectively. The procedure was validated for use in the determination of saccharin in low calorie and dietary products giving reproducible and accurate results.
Capitán-Vallvey
L F
LF
Department of Analytical Chemistry, Faculty of Science, University of Granada, Spain. lcapitan@ugr.es
Valencia
M C
MC
Nicolás
E Arana
EA
D001628:Beverages / Q000032:analysis; D004867:Equipment Design; D005504:Food Analysis / Q000379:methods; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D012439:Saccharin / Q000032:analysis*; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods*; D013549:Sweetening Agents / Q000032:analysis*
D013549:Sweetening Agents; D012439:Saccharin