title: Effect of acaricides on the activity of a Boophilus microplus glutathione S-transferase.
abstract: In the present study, we report the effect of several acaricides on the enzyme activity of a Boophilus microplus recombinant glutathione S-transferase (rGST). GST was expressed in Escherichia coli and was purified with glutathione (GSH) affinity column chromatography. The kinetic constants were determined by reacting GST with the substrates 1-chloro-2,4-dinitrobenzene (CDNB) and glutathione. We report the effect of several acaricides on the enzyme activity of rGST. Some acaricides (ethion, amitraz, chlorpyrifos, DDT, cypermethrin, diazinon, ivermectin, deltamethrin and flumethrin) inhibited rGST. Contrarily, coumaphos had an activating effect. Although the accurate mechanisms of the B. microplus resistance to acaricides remain elusive, this work helps in understanding how acaricides can interact with GST.
authors.affiliation: Centro de Biotecnologia do Estado do Rio Grande do Sul, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, C.P. 15005, 91501-970 Porto Alegre, RS, Brazil.
authors.forename: Itabajara
authors.initials: I
authors.lastname: da Silva Vaz
authors.forename: Tiago
authors.initials: T
authors.lastname: Torino Lermen
authors.forename: André
authors.initials: A
authors.lastname: Michelon
authors.forename: Carlos Alexandre
authors.initials: CA
authors.lastname: Sanchez Ferreira
authors.forename: Daniela Reis
authors.initials: DR
authors.lastname: Joaquim de Freitas
authors.forename: Carlos
authors.initials: C
authors.lastname: Termignoni
authors.forename: Aoi
authors.initials: A
authors.lastname: Masuda
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002846:Chromatography, Affinity; D004137:Dinitrochlorobenzene / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007306:Insecticides / Q000494:pharmacology*; D026863:Ixodidae / Q000201:enzymology* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D004137:Dinitrochlorobenzene; D007306:Insecticides; D011994:Recombinant Proteins; D005982:Glutathione Transferase