Lipid lateral diffusion in ordered and disordered phases in raft mixtures.
Lipid lateral diffusion coefficients in the quarternary system of dioleoylphosphatidylcholine (DOPC), sphingomyelin, cholesterol, and water were determined by the pulsed field gradient NMR technique on macroscopically aligned bilayers. The molar ratio between dioleoylphosphatidylcholine and sphingomyelin was set to 1:1, the cholesterol content was varied between 0 and 45 mol %, the water content was 40 wt %, and the temperature was varied between 293 and 333 K. The diffusion coefficients were separated into fast and slow spectral components by using the CORE method for global analysis of correlated spectral data. A large two-phase region, tentatively assigned to the liquid disordered (l(d)) and the liquid ordered (l(o)) phases, was present in the phase diagram. The l(d) phase was enriched in dioleoylphosphatidylcholine and exhibited diffusion coefficients that were about three to five times larger than for the l(o) phase. Both the diffusion coefficients and the apparent activation energies for the quarternary systems were compatible with earlier reports on ternary phospholipid/cholesterol/water systems. However, in contrast to the latter ternary systems, the exchange of lipids between the l(o) and the l(d) phases was slow on the timescale for the diffusion experiment for the quarternary ones. This means that on the millisecond timescale fluid, ordered domains are floating around in a sea of faster diffusing lipids, assigned to consist of mainly dioleoylphosphatidylcholine.
Filippov
Andrey
A
Department of Biophysical Chemistry, Umeå University, SE-901 87 Umeå, Sweden.
Orädd
Greger
G
Lindblom
Göran
G
D002784:Cholesterol / Q000737:chemistry*; D045424:Complex Mixtures / Q000737:chemistry; D004058:Diffusion; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000737:chemistry*; D021962:Membrane Microdomains / Q000737:chemistry* / Q000648:ultrastructure*; D008968:Molecular Conformation; D044367:Phase Transition; D010713:Phosphatidylcholines / Q000737:chemistry*; D013109:Sphingomyelins / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D045424:Complex Mixtures; D046911:Macromolecular Substances; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D013109:Sphingomyelins; D014867:Water; D002784:Cholesterol; C017251:1,2-oleoylphosphatidylcholine