title: Escherichia coli YidC is a membrane insertase for Sec-independent proteins.
abstract: YidC is a recently discovered bacterial membrane protein that is related to the mitochondrial Oxa1p and the Alb3 protein of chloroplasts. These proteins are required in the membrane integration process of newly synthesized proteins that do not require the classical Sec machinery. Here we demonstrate that YidC is sufficient for the membrane integration of a Sec-independent protein. Microgram amounts of the purified single-spanning Pf3 coat protein were efficiently inserted into proteoliposomes containing the purified YidC. A mutant Pf3 coat protein with an extended hydrophobic region was inserted independently of YidC into the membrane both in vivo and in vitro, but its insertion was accelerated by YidC. These results show that YidC can function separately from the Sec translocase to integrate membrane proteins into the lipid bilayer.
authors.affiliation: Institute of Microbiology and Molecular Biology, University of Hohenheim, Stuttgart, Germany.
authors.forename: Justyna
authors.initials: J
authors.lastname: Serek
authors.forename: Gabriele
authors.initials: G
authors.lastname: Bauer-Manz
authors.forename: Gabriele
authors.initials: G
authors.lastname: Struhalla
authors.forename: Lambertus
authors.initials: L
authors.lastname: van den Berg
authors.forename: Dorothee
authors.initials: D
authors.lastname: Kiefer
authors.forename: Ross
authors.initials: R
authors.lastname: Dalbey
authors.forename: Andreas
authors.initials: A
authors.lastname: Kuhn
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D036022:Capsid Proteins / Q000737:chemistry / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000502:physiology*; D008081:Liposomes / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000378:metabolism / Q000502:physiology*; D011510:Proteolipids / Q000378:metabolism; D000069816:SEC Translocation Channels; D000081416:SecA Proteins
chemical_list: D001426:Bacterial Proteins; D036022:Capsid Proteins; D029968:Escherichia coli Proteins; D008081:Liposomes; D026901:Membrane Transport Proteins; D011510:Proteolipids; D000069816:SEC Translocation Channels; C412443:YIDC protein, E coli; C020485:proteoliposomes; C084332:major coat protein, Pseudomonas phage Pf3; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins