title: To knockout in 129 or in C57BL/6: that is the question.
abstract: Traditionally, knockout experiments are performed in ES cells derived from the 129 mouse strain, followed by backcrossing with the more robust C57BL/6 strain. C57BL/6-derived ES cells have only occasionally been used in this process. We compared C57BL/6- with 129-derived ES cells directly and reviewed the literature. We found that, although some steps are less efficient, the advantages of C57BL/6 mice more than compensate for these drawbacks.
authors.affiliation: Mental Health Research Institute and Neuroscience Program, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: Eunju
authors.initials: E
authors.lastname: Seong
authors.forename: Thomas L
authors.initials: TL
authors.lastname: Saunders
authors.forename: Colin L
authors.initials: CL
authors.lastname: Stewart
authors.forename: Margit
authors.initials: M
authors.lastname: Burmeister
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D018014:Gene Transfer Techniques; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008845:Microinjections; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*