title: TRF2 is in neuroglial cytoplasm and induces neurite-like processes.
abstract: TRF2 is a ubiquitous protein that protects telomeres in the nucleus. We found that TRF2 was present at the peripheral nerve axons and the brain neuroglial cell processes extensively. It was in the cytoplasmic membrane as well as nuclear fractions, but not in the soluble cytoplasmic fraction of SH-SY5Y neuroblastoma cells. TRF2 was up-regulated in P19 embryonal carcinoma cells at the early stage of induced neural differentiation with retinoic acid treatment. Upon transfection, TRF2-expressing COS cells often produced neurite-like long cytoplasmic processes. TRF2 is a component of neuroglial cells and appears to be involved in the cytoplasmic process formation that is necessary for neural differentiation.
authors.affiliation: Asan Institute for Life Sciences, University of Ulsan College of Medicine, 388-1 Poongnap-dong, Songpa-Gu, Seoul 138-736, South Korea.
authors.forename: Yusun
authors.initials: Y
authors.lastname: Jung
authors.forename: Seungku
authors.initials: S
authors.lastname: Lee
authors.forename: Seunghyun
authors.initials: S
authors.lastname: Bang
authors.forename: Soonok
authors.initials: S
authors.lastname: Kim
authors.forename: Kyungho
authors.initials: K
authors.lastname: Choi
authors.forename: Changhee
authors.initials: C
authors.lastname: Lee
authors.forename: Seong-Gene
authors.initials: SG
authors.lastname: Lee
authors.forename: Chong Jai
authors.initials: CJ
authors.lastname: Kim
authors.forename: Kyuyoung
authors.initials: K
authors.lastname: Song
authors.forename: Inchul
authors.initials: I
authors.lastname: Lee
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology / Q000648:ultrastructure; D001921:Brain / Q000166:cytology; D019556:COS Cells; D002454:Cell Differentiation / Q000187:drug effects; D002467:Cell Nucleus / Q000502:physiology / Q000648:ultrastructure; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003593:Cytoplasm / Q000502:physiology; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D016501:Neurites / Q000502:physiology*; D009457:Neuroglia / Q000166:cytology / Q000502:physiology*; D009928:Organ Specificity; D035341:Telomeric Repeat Binding Protein 2 / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D035341:Telomeric Repeat Binding Protein 2; D014212:Tretinoin