title: ADAM (a disintegrin and metalloprotease) 12 is expressed in rat and human brain and localized to oligodendrocytes.
abstract: ADAM12 is a member of the large family of multidomain metalloprotease-disintegrins which possess cell-binding and metalloprotease properties. Typically, ADAM12 is expressed in mesenchymal cells, developing and regenerating heart and skeletal muscle, bone as well as in certain tumours. This report shows by means of reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry that the protease ADAM12 is detectable in human and rat brain tissue as well as in cultured cells derived from rat brain. With the exception of a very few immunopositive pyramidal neurons in the developing rat brain, the cellular localization of ADAM12 was exclusively confined to oligodendroglial cells. Thus, ADAM12 may be regarded a new suitable marker for this cell type.
authors.affiliation: Department of Psychiatry, University of Magdeburg, Magdeburg, Germany. Hans-Gert.Bernstein@medizin.uni-magdeburg.de
authors.forename: Hans-Gert
authors.initials: HG
authors.lastname: Bernstein
authors.forename: Gerburg
authors.initials: G
authors.lastname: Keilhoff
authors.forename: Alicja
authors.initials: A
authors.lastname: Bukowska
authors.forename: Anke
authors.initials: A
authors.lastname: Ziegeler
authors.forename: Sieglinde
authors.initials: S
authors.lastname: Funke
authors.forename: Henrik
authors.initials: H
authors.lastname: Dobrowolny
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Kanakis
authors.forename: Bernhard
authors.initials: B
authors.lastname: Bogerts
authors.forename: Uwe
authors.initials: U
authors.lastname: Lendeckel
mesh_terms: D051722:ADAM Proteins; D000072199:ADAM12 Protein; D000818:Animals; D015415:Biomarkers; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D008666:Metalloendopeptidases / Q000096:biosynthesis*; D008875:Middle Aged; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015415:Biomarkers; D008565:Membrane Proteins; D051722:ADAM Proteins; D000072199:ADAM12 Protein; C000606480:ADAM12 protein, human; D008666:Metalloendopeptidases