title: Cerebrosides of Candida lipolytica yeast.
abstract: Candida lipolytica yeast was grown batchwise on glucose medium. Cerebrosides were isolated from the sphingolipid fraction of total lipids using column chromatography and separated into two compounds by high-performance thin-layer chromatography. Glucose was detected as the sole sugar constituent in cerebrosides. The fatty acid composition of cerebrosides was characterised by a predominance of saturated fatty acids and by a high proportion of fatty acids with 16 carbon atoms. The dominant fatty acid was h16:0. The principal long-chain base components of both cerebroside species were trihydroxy bases, 18- and 20-phytosphinosine. The unique characteristic of cerebrosides was the presence of a high proportion of sphingosine (one-fourth of the total long-chain bases), which is a common characteristic of mammalian sphingolipids and rarely occurs in yeast cerebrosides. The ceramide moiety profile of cerebrosides is similar to that of epidermal ceramides, which implies a possibility for their application in care cosmetics.
authors.affiliation: Department of Chemistry and Biochemistry, Medical Faculty, University of Rijeka, B. Branchetta 20, HR-51000 Rijeka, Croatia. rjasmin@medri.hr
authors.forename: J
authors.initials: J
authors.lastname: Rupcić
authors.forename: V
authors.initials: V
authors.lastname: Marić
mesh_terms: D002175:Candida / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism*; D002518:Ceramides / Q000032:analysis; D002554:Cerebrosides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D002845:Chromatography; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D005947:Glucose / Q000378:metabolism; D013110:Sphingosine / Q000032:analysis
chemical_list: D002518:Ceramides; D002554:Cerebrosides; D005227:Fatty Acids; D005947:Glucose; D013110:Sphingosine