ORESTES are enriched in rare exon usage variants affecting the encoded proteins.
A significant fraction of the variability found in the human transcriptome is due to alternative splicing, including alternative exon usage (AEU), intron retention and use of cryptic splice sites. We present a comparison of a large-scale analysis of AEU in the human transcriptome through genome mapping of Open Reading Frame ESTs (ORESTES) and conventional ESTs. It is shown here that ORESTES probe low abundant messages more efficiently. In addition, most of the variants detected by ORESTES affect the structure of the corresponding proteins.
Sakabe
Noboru Jo
NJ
Ludwig Institute for Cancer Research, Sao Paulo Branch, Rua Prof Antonio Prudente 109, 4(o) andar, 01509-010, Sao Paulo, Brazil.
de Souza
Jorge E S
JE
Galante
Pedro A F
PA
de Oliveira
Paulo S L
PS
Passetti
Fábio
F
Brentani
Helena
H
Osório
Elisson C
EC
Zaiats
André C
AC
Leerkes
Maarten R
MR
Kitajima
João Paulo
JP
Brentani
Ricardo R
RR
Strausberg
Robert L
RL
Simpson
Andrew J G
AJ
de Souza
Sandro José
SJ
D017398:Alternative Splicing; D005091:Exons / Q000235:genetics*; D015894:Genome, Human; D006801:Humans; D016366:Open Reading Frames / Q000235:genetics*; D014158:Transcription, Genetic