Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Helicobacter pylori strain 26695.
Helicobacter pylori causes gastroduodenal disease, which is mediated in part by its outer membrane proteins (OMPs). To identify OMPs of H. pylori strain 26695, we performed a proteomic analysis. A sarcosine-insoluble outer membrane fraction was resolved by two-dimensional electrophoresis with immobilized pH gradient strips. Most of the protein spots, with molecular masses of 10 to 100 kDa, were visible on the gel in the alkaline pI regions (6.0 to 10.0). The proteome of the OMPs was analyzed by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. Of the 80 protein spots processed, 62 spots were identified; they represented 35 genes, including 16 kinds of OMP. Moreover, we identified 9 immunoreactive proteins by immunoblot analysis. This study contributes to the characterization of the H. pylori strain 26695 proteome and may help to further elucidate the biological function of H. pylori OMPs and the pathogenesis of H. pylori infection.
Baik
Seung-Chul
SC
Department of Microbiology, Gyeongsang Institute of Health Science, Gyeongsang National University College of Medicine, Jinju, Gyeongsangnam-do 660-751, Republic of Korea.
Kim
Kyung-Mi
KM
Song
Su-Min
SM
Kim
Do-Su
DS
Jun
Jin-Su
JS
Lee
Seung-Gyu
SG
Song
Jae-Young
JY
Park
Jeong-Uck
JU
Kang
Hyung-Lyun
HL
Lee
Woo-Kon
WK
Cho
Myung-Je
MJ
Youn
Hee-Shang
HS
Ko
Gyung-Hyuck
GH
Rhee
Kwang-Ho
KH
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000502:physiology; D015180:Electrophoresis, Gel, Two-Dimensional; D016480:Helicobacter pylori / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D040901:Proteomics; D012521:Sarcosine / Q000494:pharmacology*
D001425:Bacterial Outer Membrane Proteins; D012521:Sarcosine