[Basic principles of the antisense strategy].
The antisens strategy is based on specific inhibition of the mutant gene expression by oligodeoxynucleotides (OGN), capable of selectively hybridizing with target DNA or RNA. Molecular mechanisms of the antisense oligonucleotide comprise: inhibition of splicing, inhibition of 5'-capping and 3'-polyadenylation, activation of RN-ase H, small interfering RNA, ribozymes. The antisense strategy is applied to: rational design of potent, selective therapeutic agents, target validation and detection of pathologic gene expression in vivo. The main aim of the antisense oligonucleotide design is to improve the affinity for target RNA and the resistance to nucleolytic degradation.
Tauşer
Roxana Georgiana
RG
Disciplina de Chimie Farmaceutică, Facultatea de Farmacie, Universitatea de Medicină şi Farmacie Gr.T. Popa Iaşi.
Stoica
Ortansa
O
D004789:Enzyme Activation; D005786:Gene Expression Regulation; D006801:Humans; D009154:Mutation; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics*; D026723:Polyadenylation; D012326:RNA Splicing / Q000235:genetics; D016337:RNA, Catalytic / Q000378:metabolism; D012335:RNA, Ribosomal / Q000235:genetics; D034741:RNA, Small Interfering / Q000378:metabolism; D016914:Ribonuclease H / Q000378:metabolism
D020319:Oligodeoxyribonucleotides, Antisense; D016337:RNA, Catalytic; D012335:RNA, Ribosomal; D034741:RNA, Small Interfering; D016914:Ribonuclease H