Comparison of repair techniques in rat duodonal perforations: simple closure, simple closure and omentoplasty, and fibrin tissue adhesive.
BACKGROUND
We compared three repair techniques, namely, simple closure and omentoplasty, simple closure alone, and fibrin tissue adhesive, in the treatment of rat duodenal perforations induced in the postpyloric region.


METHODS
Thirty male Sprague-Dawley rats (210-240 g) were included. Under ketamine and ether anesthesia, duodenal perforations of 2 mm were induced in all rats in the postpyloric region. The rats were assigned to three groups equal in number, which underwent repair with the use of simple closure and omentoplasty, simple closure alone, and fibrin glue, respectively. All the animals were sacrificed under deep ether anesthesia on the fourth postoperative day. To assess wound strength, bursting pressure measurements were performed. In addition, hydroxyproline content of the wound site was measured by absorbance spectrophotometry at 560 nm.


RESULTS
The mean bursting pressure in the simple closure and omentoplasty group was significantly higher than those of the simple closure (p<0.05) and fibrin glue (p<0.001) groups. No significant difference existed between the simple closure and fibrin glue groups in this respect (p>0.05). Hydroxyproline values of the simple closure and omentoplasty (p<0.05) and simple closure (p<0.001) groups were significantly lower than that of the fibrin glue group.


CONCLUSIONS
Fibrin tissue adhesives may have an application in the treatment of duodenal ulcer perforations, as an adjunct to laparoscopic or open surgical methods.
Kaya
Cemal
C
Department of General Surgery, Sişli Etfal Research and Training Hospital, Istanbul, Turkey. cemal_kaya@mynet.com
Demir
Uygar
U
Coşkun
Halil
H
Kalyoncu
Ali
A
Gündüz
Burhan
B
Eroğlu
Tülay
T
Ağca
Birol
B
Mihmanli
Mehmet
M
D000818:Animals; D013505:Digestive System Surgical Procedures / Q000379:methods*; D008297:Male; D009852:Omentum / Q000601:surgery; D010439:Peptic Ulcer Perforation / Q000601:surgery*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014014:Tissue Adhesives; D014945:Wound Healing
D014014:Tissue Adhesives