[Determination of amanitatoxins by HPLC].
High-performance liquid chromatographic (HPLC) assay has been developed for the simultaneous determination of alpha-amanitin, beta-amanitin and phalloidin in serum. Three toxins were extracted by reflux in a water bath at 80 degrees C for one hour and purified by Sep-Pak Plus tC18 cartridges. The HPLC assay was performed under gradient conditions using Develosil RP AQUEOUS column. The moble phase consisted with a mixture of acetonitorile containing 0.01 M ammonium acetate(pH 5.0). The column effluence was monitored at 295 nm, 302 nm and 230 nm for 35 min. Detection limit of three toxins in serum were 0.2 microgram/ml respectively. High recovery yields in the range of 81.5-88.1% for toxins were obtained by using this method.
Nishizawa
Chiemi
C
Nagano Research Institute for Health and Pollution.
Yamaura
Yoshio
Y
D000546:Amanitins / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015925:Cryopreservation; D006801:Humans; D010590:Phalloidine / Q000097:blood*; D013048:Specimen Handling; D013997:Time Factors
D000546:Amanitins; D010590:Phalloidine; C049197:beta-amanitin