Induction of p53-independent apoptosis by the BH3-only protein ITM2Bs.
The p53 tumor suppressor protein is critically involved in cell cycle regulation and programmed cell death. Here we show that expression of the BH3-only protein ITM2Bs is able to induce apoptotic cell death in p53+/+, as well as in p53-/- cell lines. This cell death involves neither subcellular redistribution of p53 nor transcriptional regulation of p53 target genes such as Bax, Ras, Puma or Bcl-2. Together, our data provide evidence for a p53-independent apoptotic role of ITM2Bs.
Fleischer
Aarne
A
Laboratoire d'Immunologie Cellulaire et Tissulaire, INSERM U543, Bâtiment CERVI, Hôpital Pitié Salpêtrière, 83 Bd de l'Hôpital, 75013 Paris, France.
Rebollo
Angelita
A
D048868:Adaptor Proteins, Signal Transducing; D000682:Amyloid; D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D002467:Cell Nucleus / Q000502:physiology; D003600:Cytosol / Q000502:physiology; D006801:Humans; D020014:K562 Cells; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D008928:Mitochondria / Q000502:physiology; D011994:Recombinant Proteins / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency / Q000235:genetics / Q000502:physiology*
D048868:Adaptor Proteins, Signal Transducing; D000682:Amyloid; C120083:ITM2B protein, human; C492300:Itm2b protein, mouse; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; D016159:Tumor Suppressor Protein p53