Green fluorescent protein as a visual selection marker for papaya (Carica papaya L.) transformation.
Chemical-based selection for plant transformation is associated with a number of real and perceived problems that might be avoided through visual selection. We have used green fluorescent protein (GFP), as a visual selectable marker to produce transformed papaya ( Carica papaya) plants following microprojectile bombardment of embryogenic callus. GFP selection reduced the selection time from 3 months on a geneticin (G418) antibiotic-containing medium to 3-4 weeks. Moreover, GFP selection increased the number of transformed papaya plants by five-to eightfold compared to selection in the presence of antibiotics. Overall, the use of GFP for selecting transgenic papaya lines improved our throughput for transformation by 15- to 24-fold while avoiding the drawbacks associated with the use of antibiotic resistance-based selection markers.
Zhu
Y J
YJ
Hawaii Agriculture Research Center, 99-193 Aiea Heights Drive, Aiea, Hawaii 96701, USA. jzhu@harc-hspa.com
Agbayani
R
R
Moore
P H
PH
D029441:Carica / Q000235:genetics*; D002874:Chromosome Mapping; D003470:Culture Media; D005819:Genetic Markers; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008027:Light; D008164:Luminescent Proteins; D030821:Plants, Genetically Modified / Q000235:genetics*; D014170:Transformation, Genetic
D003470:Culture Media; D005819:Genetic Markers; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins