Identification and subcellular localization of neuronal calcium sensor-1 (NCS-1) in human neutrophils and HL-60 cells.
Secretion in neutrophils is thought to be regulated in different ways for the different granule types. Specific granules are endowed with proteins which are related to docking and fusion events and are absent on azurophilic granules. Furthermore, even if secretion of content from all neutrophil granules is a Ca(2+)-dependent process, a higher concentration of cytosolic calcium is required for azurophilic than for specific granule secretion. In this paper we show that human neutrophils and promyelocitic cells express neuronal calcium sensor-1 (NCS-1), a calcium binding protein involved in exocytosis in various cell types. Both mRNA and protein were found in mature cells and precursors. NCS-1 is shown to be mainly associated with azurophilic granules and, therefore could play an instrumental role in the calcium-dependent secretion of azurophilic granules.
Brochetta
Cristiana
C
Dipartimento di Fisiologia e Patologia, Università di Trieste, Italy.
Perrotta
Maria Giovanna
MG
Jeromin
Andreas
A
Romano
Maurizio
M
Vita
Francesca
F
Soranzo
Maria Rosa
MR
Borelli
Violetta
V
Roder
John
J
Zabucchi
Giuliano
G
D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D002451:Cell Compartmentation; D002458:Cell Fractionation; D002478:Cells, Cultured; D003594:Cytoplasmic Granules / Q000737:chemistry; D018922:HL-60 Cells / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D008853:Microscopy; D051580:Neuronal Calcium-Sensor Proteins; D009479:Neuropeptides / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D009504:Neutrophils / Q000737:chemistry* / Q000648:ultrastructure; D012333:RNA, Messenger / Q000032:analysis; D014158:Transcription, Genetic
D002135:Calcium-Binding Proteins; D051580:Neuronal Calcium-Sensor Proteins; D009479:Neuropeptides; D012333:RNA, Messenger; C101464:frequenin calcium sensor proteins