title: Interactions between light-induced currents, voltage-gated currents, and input signal properties in Drosophila photoreceptors.
abstract: Voltage-gated K(+) channels are important in neuronal signaling, but little is known of their interactions with receptor currents or their behavior during natural stimulation. We used nonparametric and parametric nonlinear modeling of experimental responses, combined with Hodgkin-Huxley style simulation, to examine the roles of K(+) channels in forming the responses of wild-type (WT) and Shaker mutant (Sh(14)) Drosophila photoreceptors to naturalistic stimulus sequences. Naturalistic stimuli gave results different from those of similar experiments with white noise stimuli. Sh(14) responses were larger and faster than WT. Simulation indicated that, in addition to eliminating the Shaker current, the mutation changed the current flowing through light-dependent channels [light-induced current (LIC)] and increased the delayed rectifier current. Part of the change in LIC could be attributed to direct feedback from the voltage-sensitive ion channels to the light-sensitive channels by the membrane potential. However, we argue that other changes occur in the light detecting machinery of Sh(14) mutants, possibly during photoreceptor development.
authors.affiliation: Physiological Laboratory, University of Cambridge, Cambridge CB2 1TN, United Kingdom.
authors.forename: Jeremy E
authors.initials: JE
authors.lastname: Niven
authors.forename: Mikko
authors.initials: M
authors.lastname: Vähäsöyrinki
authors.forename: Mikko
authors.initials: M
authors.lastname: Juusola
authors.forename: Andrew S
authors.initials: AS
authors.lastname: French
mesh_terms: D000818:Animals; D003624:Darkness; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology; D004331:Drosophila melanogaster; D008029:Lighting / Q000379:methods*; D020125:Mutation, Missense / Q000502:physiology; D017956:Photoreceptor Cells, Invertebrate / Q000502:physiology*; D015221:Potassium Channels / Q000235:genetics / Q000502:physiology*; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics / Q000502:physiology; D051637:Shaker Superfamily of Potassium Channels
chemical_list: D029721:Drosophila Proteins; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; C496929:Sh protein, Drosophila; D051637:Shaker Superfamily of Potassium Channels