Vibrio hispanicus sp. nov., isolated from Artemia sp. and sea water in Spain.
Three Gram-negative, small, motile, rod-shaped bacteria were isolated from Artemia sp. and sea water in Barcelona, Spain, during 1990 and 1991. They were fermentative, oxidase-positive, sensitive to vibriostatic agent O/129, arginine dihydrolase-positive, lysine and ornithine decarboxylase-negative and grew in the absence of NaCl. They differed from phenotypically related species by their ability to grow at 4 degrees C and utilize L-rhamnose. Cloning of the 16S rRNA gene of the type strain produced two different 16S rRNA gene sequences, which differed by 15 bases (0.99%); comparison of these sequences with those deposited in GenBank showed close relationships with Vibrio proteolyticus (97.6% similarity), Vibrio diazotrophicus (97.9%), Vibrio campbellii (96.8%) and Vibrio alginolyticus (96.8%), among others. DNA-DNA hybridization levels with the closest phylogenetically related Vibrio species were <26.4%. Sufficient evidence is provided to support the identity of the three strains analysed as members of a novel species of the genus Vibrio, for which the name Vibrio hispanicus sp. nov. is proposed, with the type strain LMG 13240T (=CAIM 525T=VIB 213T).
Gomez-Gil
B
B
CIAD/Mazatlán Unit for Aquaculture, AP. 711, Mazatlán Sinaloa, Mexico 82000.
Thompson
F L
FL
BCCMTM/LMG Bacteria Collection, Laboratory for Microbiology, Ghent University, Ghent 9000, Belgium.|Laboratory for Microbiology, Ghent University, Ghent 9000, Belgium.
Thompson
C C
CC
Laboratory for Microbiology, Ghent University, Ghent 9000, Belgium.
Garcia-Gasca
A
A
CIAD/Mazatlán Unit for Aquaculture, AP. 711, Mazatlán Sinaloa, Mexico 82000.
Roque
A
A
CIAD/Mazatlán Unit for Aquaculture, AP. 711, Mazatlán Sinaloa, Mexico 82000.
Swings
J
J
BCCMTM/LMG Bacteria Collection, Laboratory for Microbiology, Ghent University, Ghent 9000, Belgium.|Laboratory for Microbiology, Ghent University, Ghent 9000, Belgium.
D000818:Animals; D001156:Artemia / Q000382:microbiology*; D006090:Gram-Negative Bacteria / Q000145:classification / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009955:Ornithine Decarboxylase / Q000235:genetics; D010641:Phenotype; D010802:Phylogeny; D012623:Seawater / Q000382:microbiology*; D014733:Vibrio / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D009955:Ornithine Decarboxylase