title: Monitoring the formation of Maillard reaction products of glucosamine with fibrinogen and human serum albumin using capillary electrophoresis.
abstract: Formation of Maillard reaction products (MRP) of glucosamine (GlcN) with fibrinogen and human serum albumin (HSA), under simulated physiological conditions, was detected by fluorescence (excitation/emission: 340/420 nm) and UV/Vis (max. 275 nm) spectroscopy. The application of polyacrylamide gel electrophoresis demonstrated the generation of high-molecular-weight fibrinogen and HSA MRP by GlcN. A simple and rapid capillary electrophoresis method was developed to separate MRP formed by the reaction of GlcN with proteins from GlcN autocondensation products.
authors.affiliation: Department of Chemistry, University of Rhode Island, Kingston, RI 02881, USA.
authors.forename: Xinfeng
authors.initials: X
authors.lastname: Zhang
authors.forename: Yue
authors.initials: Y
authors.lastname: Ma
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Paula F G
authors.initials: PF
authors.lastname: de Sa
authors.forename: Phyllis R
authors.initials: PR
authors.lastname: Brown
authors.forename: Joel A
authors.initials: JA
authors.lastname: Dain
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen / Q000378:metabolism*; D005944:Glucosamine / Q000378:metabolism*; D017127:Glycation End Products, Advanced / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D015416:Maillard Reaction; D012709:Serum Albumin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D017127:Glycation End Products, Advanced; D012709:Serum Albumin; D005340:Fibrinogen; D005944:Glucosamine