[Evaluation of molecular weights of hyaluronate preparations by multi-angle laser light scattering].
Hyaluronate (HA), a glycosaminoglycan polysaccharide, has been used for osteoarthritis, periartritis of the shoulder and rheumatoid arthritis by intraarticular administration, and in ophthalmic surgery such as anterior segment surgery, and eye lotion. In this study, the molecular weight (Mw) of HA preparations were estimated by size-exclusion chromatography (SEC) system consisted of a refractometer (RI) and a multi-angle laser light scattering (MALS). From the results, it has been clarified that a successful characterization of HA samples with Mw up to 2 - 3 x 10(6) g/mol was possible by multidetector system.
Yomota
Chikako
C
yomota@nihs.go.jp
D002850:Chromatography, Gel / Q000295:instrumentation* / Q000379:methods; D004304:Dosage Forms; D006820:Hyaluronic Acid / Q000737:chemistry*; D007834:Lasers; D008970:Molecular Weight; D012031:Refractometry / Q000295:instrumentation; D012542:Scattering, Radiation
D004304:Dosage Forms; D006820:Hyaluronic Acid