title: Determination of ochratoxin A in wine using liquid-phase microextraction combined with liquid chromatography with fluorescence detection.
abstract: A liquid-liquid microextraction technique (LPME) has been applied to the extraction of ochratoxin A (OTA) from wine prior to its quantification by HPLC-fluorescence detection. OTA was extracted from wine, through 1-octanol immobilized in the pores of a porous hollow fiber, and introduced into 1-octanol inside the fiber. Recovery was 77%. The method was adequate for quantification of OTA in wine at levels within the range 0.25-10 ng/ml with a LOD of 0.2 ng/ml, and can be a simple and inexpensive alternative to the use of inmunoaffinity columns in order to quantify OTA levels in wine.
authors.affiliation: Organic and Pharmaceutical Chemistry Department, Faculty of Pharmacy, University of Navarra, C/Irunlarrea s/n, 31008 Pamplona, Spain. mgpenas@unav.es
authors.forename: E
authors.initials: E
authors.lastname: González-Peñas
authors.forename: C
authors.initials: C
authors.lastname: Leache
authors.forename: M
authors.initials: M
authors.lastname: Viscarret
authors.forename: A
authors.initials: A
authors.lastname: Pérez de Obanos
authors.forename: C
authors.initials: C
authors.lastname: Araguás
authors.forename: A
authors.initials: A
authors.lastname: López de Cerain
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009793:Ochratoxins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014920:Wine / Q000032:analysis*
chemical_list: D009793:Ochratoxins; C025589:ochratoxin A