title: Methods in functional proteomics: two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients, in-gel digestion and identification of proteins by mass spectrometry.
authors.affiliation: Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO, USA.
authors.forename: Karine R
authors.initials: KR
authors.lastname: Bernard
authors.forename: Karen R
authors.initials: KR
authors.lastname: Jonscher
authors.forename: Katheryn A
authors.initials: KA
authors.lastname: Resing
authors.forename: Natalie G
authors.initials: NG
authors.lastname: Ahn
mesh_terms: D000478:Alkylation; D002021:Buffers; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007091:Image Processing, Computer-Assisted; D007525:Isoelectric Focusing / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D010084:Oxidation-Reduction; D010449:Peptide Mapping / Q000379:methods; D011506:Proteins / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013194:Staining and Labeling / Q000379:methods; D014357:Trypsin
chemical_list: D002021:Buffers; D011506:Proteins; D014357:Trypsin