Pulmonary function in rats dying from long-term parenteral nutrition.
Infusion of Vamin or Intralipid causes death in a rat model of continuous parenteral nutrition. Morphological investigations have shown vascular injury and thrombus formation in the lungs. In this study, lung function in rats was examined before death due to parenteral nutrition. The rats were fed saline intravenously (group I); 100 mL kg(-1) day(-1) (controls); a 7% amino acid-glucose solution (Vamin-Glukos) (group II); 100 mL kg(-1) day(-1), or 20% fat emulsion (Intralipid) (group III); 40 mL kg(-1) day(-1). The infusion was stopped when the condition of the rats deteriorated. In a saline-perfused, isolated lung model, pulmonary arterial pressure (Ppa), transpulmonary pressure (Ptp), endothelial function, measured as inactivation of serotonin (bioassay), and the capillary filtration coefficient (CFC) were determined. Haematological parameters were also evaluated. Constant findings in group II and III were central thrombus formation, anaemia and thrombocytopenia. Ppa increased from 0.7 (0.04) kPa in group I to 1.4 (0.1) kPa and 1.7 (0.1) kPa in groups II and III, respectively (p<0.001). Inactivation of serotonin was reduced to 36% (2) in group II and 37% (2) in group III compared with 74% (5) in group I (p<0.002). CFC increased to 25 mg min(-1) (5) (group II) and 30 mg min(-1) (6) (group III) compared with 13 mg min(-1) (2) in controls (p=0.01). The study shows that major pulmonary hypertension and severe reduction of the endothelial function are present when rats deteriorate after infusion of parenteral nutrition substrates.
Dahl
P E
PE
Department of Surgery, Institute of Clinical Medicine, University Hospital of North Norway, Tromsø, Norway. per.erling.dahl@unn.no
Kjaeve
J C
JC
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D001772:Blood Cell Count; D003328:Coronary Thrombosis / Q000175:diagnosis; D004573:Electrolytes; D005217:Fat Emulsions, Intravenous / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D007263:Infusions, Parenteral; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D010288:Parenteral Nutrition / Q000009:adverse effects*; D057947:Parenteral Nutrition Solutions; D010976:Platelet Count; D051381:Rats; D017208:Rats, Wistar; D012129:Respiratory Function Tests; D012701:Serotonin / Q000493:pharmacokinetics; D012996:Solutions; D013997:Time Factors
D000596:Amino Acids; D004573:Electrolytes; D005217:Fat Emulsions, Intravenous; D057947:Parenteral Nutrition Solutions; D012996:Solutions; C545824:amino-acid, glucose, and electrolyte solution; D012701:Serotonin; D005947:Glucose