title: Higher vessel densities in retinoblastoma with local invasive growth and metastasis.
abstract: In this study, the importance of angiogenesis (the growth of new blood vessels from existing ones) for the growth of retinoblastoma was investigated by a retrospective immunohistochemical analysis. An individual vessel index for each tumor was determined using the endothelial-specific antibody CD 31 for vessel staining. The obtained data were correlated with clinical features, pathohistological characteristics, and the presence of metastasis. In 107 retinoblastomas collected between 1980 and 1990, we found no difference in the vessel densities between uni- and bilateral retinoblastomas (P = 0.41). However, tumors that had invaded the chorioid and/or the optic nerve statistically showed higher vessel densities than tumors without local invasive growth (P = 0.05 and P = 0.024). A tendency of higher vessel densities in retinoblastomas presenting with metastasis at the time of diagnosis was observed (P = 0.11). Based on this observation, we proceeded to examine all retinoblastomas presenting with metastasis at the time of diagnosis. These included patients that were treated between 1968 and 1993. The 18 investigated retinoblastomas had significantly higher vessel densities than all other retinoblastomas presenting without metastasis (P = 0.025). Our data indicate that in retinoblastoma, blood vessels are essential for local and systemic invasive growth. Therefore, an anti-angiogenic therapy could be considered in the multimodal therapy concept for retinoblastomas with invasive growth, both locally and systemically.
authors.affiliation: Department of Pediatric Hematology, Oncology and Endocrinology, University Hospital of Essen, Essen, Germany. roessler@kikli.ukl.uni-freiburg.de
authors.forename: Jochen
authors.initials: J
authors.lastname: Rössler
authors.forename: Torsten
authors.initials: T
authors.lastname: Dietrich
authors.forename: Helena
authors.initials: H
authors.lastname: Pavlakovic
authors.forename: Lothar
authors.initials: L
authors.lastname: Schweigerer
authors.forename: Werner
authors.initials: W
authors.lastname: Havers
authors.forename: Andreas
authors.initials: A
authors.lastname: Schüler
authors.forename: Norbert
authors.initials: N
authors.lastname: Bornfeld
authors.forename: Harald
authors.initials: H
authors.lastname: Schilling
mesh_terms: D004730:Endothelium, Vascular / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D009361:Neoplasm Invasiveness; D009389:Neovascularization, Pathologic; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D019572:Retinal Neoplasms / Q000098:blood supply* / Q000473:pathology*; D012175:Retinoblastoma / Q000098:blood supply* / Q000473:pathology*; D012189:Retrospective Studies
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1