Molecular cloning and characterization of a new Na+/H+ antiporter gene from Brassica napus.
A new Na+/H+ vacuolar antiporter gene from Brassica napus was cloned. The full-length cDNA of B. napus antiporter gene (BnNHX1, GenBank Acc. No. AY189676) was 1819 bp and contained a 1545 bp open reading frame encoding a protein of 515 amino acids. Homology analysis and molecular modeling revealed that BnNHX1 strongly resembled other Na+/H+ antiporter genes. Its encoded protein belonged to a typical sodium/hydrogen exchanger superfamily and shared consensus sequences within predicted transmemberane segments. Southern blot analysis indicated there were multi-copies of BnNHX1 gene present in B. napus genome, which was different from that in Oryza sativa previously reported. Northern blot analysis revealed that BnNHX1 was salt-inducible and its transcript level was most abundant after 24 h treatment with 200 mM sodium chloride. Our studies suggested that BnNHX1 was a new member of the family of recently cloned plant NHX-genes.
Wang
Jin
J
State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai 200433, People's Republic of China.
Zuo
Kaijing
K
Wu
Weisheng
W
Song
Jun
J
Sun
Xiaofen
X
Lin
Juan
J
Li
Xufeng
X
Tang
Kexuan
K
D000595:Amino Acid Sequence; D029688:Brassica napus / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics*
D017923:Sodium-Hydrogen Exchangers