title: Lactational exposure of Han/Wistar rats to 2,3,7,8-tetrachlorodibenzo-p-dioxin interferes with enamel maturation and retards dentin mineralization.
abstract: Exposure to environmental dioxins via mother's milk may be one causative factor of mineralization defects in children's teeth. A prerequisite for the completion of enamel mineralization is the removal of enamel matrix. To test the hypothesis that dioxins interfere with enamel maturation, we administered lactating Han/Wistar rats a single dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; 50 or 1000 micro g/kg) on the day after delivery and analyzed tissue sections of the pup heads at post-natal days (Pn) 9 and 22. By Pn22, the first and second molars of the exposed pups, but not controls, showed retention of enamel matrix. Predentin was thicker than normal. Immunostaining for the aryl hydrocarbon/dioxin receptor (AhR) and cytochrome P4501A1 (CYP1A1) in ameloblasts and odontoblasts was reduced, suggesting that TCDD interferes with tooth mineralization via AhR. Extinction of AhR may lead to abolition of CYP1A1 expression as a sign of impaired dental cell function.
authors.affiliation: Department of Pedodontics and Orthodontics, Institute of Dentistry, University of Helsinki, Finland.
authors.forename: Y
authors.initials: Y
authors.lastname: Gao
authors.forename: C
authors.initials: C
authors.lastname: Sahlberg
authors.forename: A
authors.initials: A
authors.lastname: Kiukkonen
authors.forename: S
authors.initials: S
authors.lastname: Alaluusua
authors.forename: R
authors.initials: R
authors.lastname: Pohjanvirta
authors.forename: J
authors.initials: J
authors.lastname: Tuomisto
authors.forename: P-L
authors.initials: PL
authors.lastname: Lukinmaa
mesh_terms: D000565:Ameloblasts / Q000187:drug effects; D000566:Amelogenesis / Q000187:drug effects; D000818:Animals; D016009:Chi-Square Distribution; D019363:Cytochrome P-450 CYP1A1 / Q000032:analysis; D003743:Dental Enamel / Q000187:drug effects*; D003804:Dentin / Q000187:drug effects*; D004785:Environmental Pollutants / Q000009:adverse effects*; D005260:Female; D007774:Lactation; D008892:Milk; D009804:Odontoblasts / Q000187:drug effects; D000072317:Polychlorinated Dibenzodioxins / Q000009:adverse effects*; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar; D018336:Receptors, Aryl Hydrocarbon / Q000032:analysis; D014074:Tooth Calcification / Q000187:drug effects*
chemical_list: D004785:Environmental Pollutants; D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon; D019363:Cytochrome P-450 CYP1A1