title: High-throughput classification of images of cells transfected with cDNA clones.
abstract: The sequence of the human genome has been determined. The next task is to determine the function of the genes. Classifying cellular forms of proteins encoded by human cDNA clones is a primary step toward understanding the biological role of proteins and their coding genes. We report here our ongoing work on an automatic system to facilitate this classification. Our system handles the transfection, incubation, acquisition of microscopic images of the cells, and the classification of forms there appearing in the images. Our system correctly classified proteins by their forms at a rate of 90% in feasibility studies.
authors.affiliation: The Tokyo Metropolitan Institute of Medical Science, Tokyo 113-8613, Japan. tachino@rinshoken.or.jp
authors.forename: Reiko
authors.initials: R
authors.lastname: Minamikawa-Tachino
authors.forename: Noriko
authors.initials: N
authors.lastname: Kabuyama
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Gotoh
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Kagei
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Naruse
authors.forename: Yasutomo
authors.initials: Y
authors.lastname: Kisu
authors.forename: Takushi
authors.initials: T
authors.lastname: Togashi
authors.forename: Sumio
authors.initials: S
authors.lastname: Sugano
authors.forename: Hitohide
authors.initials: H
authors.lastname: Usami
authors.forename: Nobuo
authors.initials: N
authors.lastname: Nomura
mesh_terms: D000465:Algorithms; D002477:Cells / Q000145:classification; D003001:Cloning, Molecular; D018076:DNA, Complementary; D007091:Image Processing, Computer-Assisted; D011506:Proteins; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D011506:Proteins