title: Isolation and expression of the homeobox gene Gbx1 during mouse development.
abstract: In zebrafish, gbx1 and otx2 are among the earliest genes expressed in the neuroectoderm, dividing it into an anterior and a posterior domain with a common border that marks the midbrain-hindbrain boundary (MHB) primordium. Here, we describe the sequence and expression pattern of Gbx1 in mouse. The first transcripts are found at embryonic day 7.75 in the hindbrain. Later on, expression of Gbx1 is detectable in the hindbrain (rhombomeres 2 to 7), spinal cord, optic vesicles, and in the ventral telencephalon. In mouse, Gbx1 expression is not observed at the MHB as is the case during early zebrafish development. We suggest that an evolutionary switch occurred: in mouse Gbx2 is involved in the early specification of the MHB primordium, whereas in zebrafish, gbx1 is required instead of gbx2.
authors.affiliation: Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Muriel
authors.initials: M
authors.lastname: Rhinn
authors.forename: Klaus
authors.initials: K
authors.lastname: Lun
authors.forename: Michaela
authors.initials: M
authors.lastname: Werner
authors.forename: Antonio
authors.initials: A
authors.lastname: Simeone
authors.forename: Michael
authors.initials: M
authors.lastname: Brand
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D051767:Early Growth Response Protein 2; D005314:Embryonic and Fetal Development / Q000235:genetics / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology*; D005801:Genes, Homeobox; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000235:genetics; D009928:Organ Specificity; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D029961:Zebrafish Proteins
chemical_list: D004268:DNA-Binding Proteins; D051767:Early Growth Response Protein 2; C497327:Egr2 protein, mouse; C490147:Gbx1 protein, mouse; C513182:Gbx2 protein, mouse; C101121:HOXB1 homeodomain protein; D018398:Homeodomain Proteins; D014157:Transcription Factors; D029961:Zebrafish Proteins; C513181:gbx2 protein, zebrafish