A selective intra-HN(CA)CO experiment for the backbone assignment of deuterated proteins.
A modified version of the backbone TROSY based 3D HN(CA)CO experiment is presented that provides uniquely intraresidue correlation shift information, by efficiently suppressing the sequential connectivity data. The resulting reduction in the number of peaks decreases overlap and removes ambiguities in situations where in the conventional HN(CA)CO i and i - 1 correlations are overlapped or are comparable in intensity. The sequential residue connection is obtained in combination with a 3D HNCO. MQ- and SQ-implementations of the experiment were tested on a deuterated protein with a correlation time of 28 ns ( approximately 60 kDa).
Nietlispach
Daniel
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Cambridge Centre for Molecular Recognition, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, UK. dn0@bioc.cam.ac.uk
D003903:Deuterium; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
D011506:Proteins; D003903:Deuterium