title: Blue light differentially modulates cell survival and growth.
abstract: Previous studies have reported that blue light (400-500 nm) inhibits cell mitochondrial activity. We investigated the hypothesis that cells with high energy consumption are most susceptible to blue-light-induced mitochondrial inhibition. We estimated cell energy consumption by population doubling time, and cell survival and growth by succinate dehydrogenase (SDH) activity. Six cell types were exposed to 5 or 60 J/cm(2) of blue light from quartz-tungsten-halogen (QTH), plasma-arc (PAC), or argon laser sources in monolayer culture. Post-light SDH activity correlated positively with population doubling time (R(2) = 0.91 for PAC, 0.76 for QTH, 0.68 for laser); SDH activity increased for cell types with the longest doubling times and was suppressed for cell types with shorter doubling times. Thus, light-induced exposure differentially affects SDH activity, cell survival, and growth, depending on cell energy consumption. Blue light may be useful as a therapeutic modulator of cell growth and survival.
authors.affiliation: Department of Oral Rehabilitation, Medical College of Georgia School of Dentistry, Augusta, GA 30912-1260, USA. watahaj@mail.mcg.edu
authors.forename: J C
authors.initials: JC
authors.lastname: Wataha
authors.forename: J B
authors.initials: JB
authors.lastname: Lewis
authors.forename: P E
authors.initials: PE
authors.lastname: Lockwood
authors.forename: S
authors.initials: S
authors.lastname: Hsu
authors.forename: R L
authors.initials: RL
authors.lastname: Messer
authors.forename: F A
authors.initials: FA
authors.lastname: Rueggeberg
authors.forename: S
authors.initials: S
authors.lastname: Bouillaguet
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology; D002277:Carcinoma / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002455:Cell Division / Q000528:radiation effects*; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000528:radiation effects*; D005260:Female; D005347:Fibroblasts / Q000201:enzymology / Q000528:radiation effects; D005881:Gingiva / Q000473:pathology; D006801:Humans; D015603:Keratinocytes / Q000201:enzymology / Q000528:radiation effects; D007834:Lasers; D008027:Light; D008029:Lighting / Q000295:instrumentation; D008168:Lung / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008928:Mitochondria / Q000528:radiation effects; D009062:Mouth Neoplasms / Q000473:pathology; D012867:Skin / Q000473:pathology; D013385:Succinate Dehydrogenase / Q000528:radiation effects
chemical_list: D013385:Succinate Dehydrogenase