Persistence of translocation frequencies in blood lymphocytes following radiotherapy: implications for retrospective radiation biodosimetry.
Chromosome aberration analysis using a G-banding technique was performed on peripheral blood lymphocyte cultures from eight individuals over a 5 year period following therapeutic radiation exposure. Samples were placed in three time periods comprising 0-12, 12-36 and 36-60 months post-treatment. The group was heterogeneous with respect to exposure and this resulted in wide differences in initial total translocation yields. Total translocation frequencies declined in seven of the eight cases, reaching significance in four cases. This decline was attributed to a decrease in cells, which in addition to translocations, also contained aberrations such as dicentrics which resulted in them being unstable. In all eight cases, when only stable cells were considered, no significant differences were observed in translocation frequencies between the different time periods post-treatment. Thus, although the frequency of translocations in stable cells is persistent over time, extrapolating to total initial yield, and using this to equate to dose, is not possible in cases where the exposure has been high and non-homogeneous. In practice, retrospective biological dosimetry is more often required in cases of historical, usually protracted, exposures which will have been essentially uniform and not of a sufficiently high dose for many cells to have acquired more than one aberration. In such cases the frequency of translocations observed some years after the exposure can be assumed to reflect induced frequencies and be used for dose estimation.
Tawn
E Janet
EJ
Genetics Unit, Westlakes Research Institute, Moor Row, Cumbria CA24 3JY, UK. Jan.Tawn@westlakes.ac.uk
Whitehouse
Caroline A
CA
D000328:Adult; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects*; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000532:radiotherapy*; D011874:Radiometry; D011879:Radiotherapy Dosage; D013997:Time Factors; D014178:Translocation, Genetic