title: Neural cell fate analysis in zebrafish using olig2 BAC transgenics.
abstract: We describe here simple methods for producing transgenic zebrafish reporter lines using BAC clones. The use of BAC clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Combined with recent advances in live embryo image analysis, this strategy has the potential to greatly advance the investigation of neural cell behavior during development.
authors.affiliation: Vanderbilt University, Department of Biological Sciences, Nashville, TN 37232, USA.
authors.forename: Jimann
authors.initials: J
authors.lastname: Shin
authors.forename: Hae-Chul
authors.initials: HC
authors.lastname: Park
authors.forename: Jolanta M
authors.initials: JM
authors.lastname: Topczewska
authors.forename: David J
authors.initials: DJ
authors.lastname: Mawdsley
authors.forename: Bruce
authors.initials: B
authors.lastname: Appel
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D008845:Microinjections; D009046:Motor Neurons / Q000166:cytology / Q000378:metabolism*; D017933:Peripheral Nervous System / Q000166:cytology / Q000378:metabolism*; D011689:Purkinje Cells / Q000166:cytology / Q000378:metabolism*; D015027:Zebrafish / Q000033:anatomy & histology / Q000235:genetics* / Q000378:metabolism
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins