title: Mineralization behaviour of collagen type I immobilized on different substrates.
abstract: Collagen type I as a robust fibre protein and main component of the extracellular matrix of most tissues is increasingly utilized for surface engineering of biomaterials using different immobilization methods. In the present work we studied the mineralization behaviour of fibrillar collagen type I in simulated body fluid as a measure for conformational changes caused by adsorptive immobilization or immobilization by partial incorporation into the anodic oxide layer on c.p.-titanium using microscopic and vibration spectroscopic methods. Adsorptive immobilization on highly oriented pyrolytic graphite (HOPG) and c.p.-titanium without collagen were used as references. In the initial phase (1-24 h) the kinetics of formation and the morphology of calcium phosphate phases (CPP) are strongly influenced both by the substrate and the immobilization method. Compared to HOPG both types of immobilization on titanium increasingly inhibit the formation of CPP. For longer times (30 d) these initial differences disappear-mineralization product on titanium, irrespective of the presence of collagen, is a mixture of amorphous calcium phosphate and octacalcium phosphate. Contrary to this the mineralization of HOPG substrates results in hydroxy apatite. This is discussed with respect to the conditions during the immobilization as well as the resulting interactions between substrate and immobilized collagen. It is shown that the mineralization process exhibits a high sensitivity with respect to conformational changes caused by these interactions. Possible cell biological relevance of these conformational changes is discussed.
authors.affiliation: Institute of Materials Science, Max-Bergmann-Zentrum fur Biomaterialien, TU Dresden, Budapester Strasse 27, Dresden 01069, Germany. dieter.scharnweber@mailbox.tu-dresden.de
authors.forename: Dieter
authors.initials: D
authors.lastname: Scharnweber
authors.forename: René
authors.initials: R
authors.lastname: Born
authors.forename: Katarina
authors.initials: K
authors.lastname: Flade
authors.forename: Sophie
authors.initials: S
authors.lastname: Roessler
authors.forename: Martina
authors.initials: M
authors.lastname: Stoelzel
authors.forename: Hartmut
authors.initials: H
authors.lastname: Worch
mesh_terms: D000327:Adsorption; D001826:Body Fluids / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D024042:Collagen Type I / Q000737:chemistry* / Q000648:ultrastructure*; D024022:Fibrillar Collagens / Q000737:chemistry / Q000648:ultrastructure; D006108:Graphite / Q000737:chemistry; D008422:Materials Testing; D008903:Minerals / Q000138:chemical synthesis*; D011487:Protein Conformation; D013329:Structure-Activity Relationship; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D024022:Fibrillar Collagens; D008903:Minerals; D006108:Graphite; D014025:Titanium