title: In vitro effects of enamel matrix proteins on rat bone marrow cells and gingival fibroblasts.
abstract: Emdogain (EMD), a formulation of Enamel Matrix Proteins (EMP), is used clinically for periodontal regeneration, where it stimulates cementum formation and promotes gingival healing. In this study, we investigated the in vitro effects of EMD on rat bone marrow stromal cells (BMSC) and gingival fibroblasts (GF). EMD (at 25 micro g/mL) increased the osteogenic capacity of bone marrow, as evidenced by approximately three-fold increase in BMSC cell number and approximately two-fold increase in alkaline phosphatase (ALP) activity and mineralized nodule formation. The presence of EMD in the initial stages (first 48 hrs) of the culture was crucial for this effect. In contrast, EMD did not induce osteoblastic differentiation of GF (evidenced by lack of mineralization or ALP activity) but increased up to two-fold both their number and the amount of matrix produced. These in vitro data on BMSC and GF could explain the promotive effect of EMD on bone formation and connective tissue regeneration, respectively.
authors.affiliation: Department of Oral Biology, The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Keila
authors.forename: C E
authors.initials: CE
authors.lastname: Nemcovsky
authors.forename: O
authors.initials: O
authors.lastname: Moses
authors.forename: Z
authors.initials: Z
authors.lastname: Artzi
authors.forename: M
authors.initials: M
authors.lastname: Weinreb
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects*; D001857:Bone Matrix / Q000187:drug effects; D002113:Calcification, Physiologic / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects*; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D008297:Male; D010012:Osteogenesis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000187:drug effects; D013997:Time Factors
chemical_list: D003746:Dental Enamel Proteins; C109069:enamel matrix proteins; D000469:Alkaline Phosphatase