title: Detection of iron(III)-binding ligands originating from marine phytoplankton using cathodic stripping voltammetry.
abstract: The sample preparation and analytical methodology are described for detecting biologically produced iron(III)-binding ligands in laboratory cultures of coastal marine phytoplankton. The iron(III)-binding ligands from the culture media were purified by passage through a column packing with a hydrophobic absorbent. The concentrations and stability constants of the ligands were determined by adsorptive cathodic stripping voltammetry with competitive ligand equilibration. The analytical results of the cultivated cultures suggest that eukaryotic phytoplankton would produce iron(III)-binding ligands in analogy with other microorganisms.
authors.affiliation: Faculty of Engineering, Kanazawa University, Kodatsuno, Kanazawa 920-8667, Japan. hhiroshi@t.kanazawa-u.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hasegawa
authors.forename: Teruya
authors.initials: T
authors.lastname: Maki
authors.forename: Kohnosuke
authors.initials: K
authors.lastname: Asano
authors.forename: Kentaro
authors.initials: K
authors.lastname: Ueda
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Ueda
mesh_terms: D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D007501:Iron / Q000378:metabolism*; D008024:Ligands; D008386:Marine Biology; D010839:Phytoplankton / Q000378:metabolism*
chemical_list: D008024:Ligands; D007501:Iron