Massive thymic deletion results in systemic autoimmunity through elimination of CD4+ CD25+ T regulatory cells.
Incomplete deletion of KRN T cells that recognize the ubiquitously expressed self-antigen glucose-6-phosphate-isomerase (GPI) initiates an anti-GPI autoimmune cascade in K/BxN mice resulting in a humorally mediated arthritis. Transgenic (Tg) expression of a KRN T cell receptor (TCR) agonist under the major histocompatibility complex class II promoter resulted in thymic deletion with loss of anti-GPI T and B cell responses and attenuated arthritis course. However, double Tg mice succumbed to systemic autoimmunity with multiorgan inflammation and autoantibody production. Extensive thymic deletion resulted in lymphopenia and elimination of CD4+ CD25+ regulatory T cells (Tregs), but spared some CD4+ T cells expressing endogenous TCR, which oligoclonally expanded in the periphery. Disease was transferred by these T cells and prevented by cotransfer of CD4+ CD25+ Tregs. Moreover, we extended our findings to another TCR system (anti-hen egg lysozyme [HEL] TCR/HEL mice) where similarly extensive thymic deletion also resulted in disease. Thus, our studies demonstrated that central tolerance can paradoxically result in systemic autoimmunity through differential susceptibility of Tregs and autoreactive T cells to thymic deletion. Therefore, too little or too much negative selection to a self-antigen can result in systemic autoimmunity and disease.
Shih
Fei F
FF
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Mandik-Nayak
Laura
L
Wipke
Brian T
BT
Allen
Paul M
PM
D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002645:Chickens; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000276:immunology; D007249:Inflammation / Q000276:immunology; D008212:Lymphocyte Depletion; D051379:Mice; D008822:Mice, Transgenic; D009113:Muramidase / Q000235:genetics; D016296:Mutagenesis; D016133:Polymerase Chain Reaction; D015375:Receptors, Interleukin-2 / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013934:Thymectomy
D015703:Antigens, CD; D017931:DNA Primers; D015375:Receptors, Interleukin-2; D009113:Muramidase