Comparative analysis of RCAS1 level in neoplasms and placenta.
The tumor associated antigen RCAS1 (receptor-binding cancer antigen expressed on SiSo cells) expressed with high frequency in various cancer and trophoblast cells, inhibits growth of estrogen receptor-expressing cells and induces apoptosis. Because previous reports demonstrated RCAS1 presence only by non-quantitative immunocytochemistry methods, we decided to use a Western blotting with anti-RCAS1 monoclonal antibodies for estimation of the relative content of the tumor-associated antigen. One hundred tissue samples were assayed (neoplasms, chronic inflammatory diseases, healthy tissues, trophoblasts and placentas at term). RCAS1 was present in all neoplastic, placental and trophoblast tissue samples and its level in malignant samples was statistically significantly higher than in benign neoplasms. The amount of RCAS1 in chronic inflammations was also significantly increased in immune mediated diseases, like allergic nasal polyps and sarcoidosis. The RCAS1 protein was not revealed in healthy mucous membrane and in muscle tissues. The presented results suggest that RCAS1 might play an important role in tumor escape from host immunological surveillance and carry weight in the down regulation of the maternal immune response, thereby maintaining pregnancy.
Wicherek
Lukasz
L
Department of Gynecology and Infertility Clinic, Jagiellonian University, Kraków, Poland. mowicher@cyf-kr.edu.pl
Dutsch
Magdalena
M
Mak
Pawel
P
Klimek
Marek
M
Skladzien
Jacek
J
Dubin
Adam
A
D000951:Antigens, Neoplasm / Q000097:blood / Q000378:metabolism*; D015153:Blotting, Western; D002277:Carcinoma / Q000378:metabolism; D005260:Female; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D009369:Neoplasms / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy
D000951:Antigens, Neoplasm; C120762:EBAG9 protein, human