title: Antibacterial titanium plate anodized by being discharged in NaCl solution exhibits cell compatibility.
abstract: Implant surfaces should be modified to achieve excellent cell compatibility as well as antibacterial activity. Our previous study demonstrated that titanium plates anodized by being discharged in NaCl (Ti-Cl) exhibited high antibacterial activity. Since Ti-Cl was prepared with a NaCl solution, we hypothesized that Ti-Cl would exhibit low toxicity toward cells. The aims of this study were to characterize the surface of Ti-Cl and investigate the cell compatibility (MC3T3-E1 and L929 cells) of Ti-Cl. The results demonstrated that, since the TiCl(3) formed on the Ti-Cl surface was hydrolyzed into HCl, HClO, and TiOH after immersion in pure distilled water, TiCl(3) contributed to the antibacterial activity of Ti-Cl. On the other hand, TiO formed on the Ti-Cl surface enhanced cell extension and cell growth through a larger adsorption of fibronectin compared with the pure titanium control. These findings suggest that antibacterial titanium is a promising material for use in dental implant systems.
authors.affiliation: Department of Oral Biomaterials and Technology, Showa University School of Dentistry, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo, 142-8555, Japan. yookun@dent.showa-u.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Shibata
authors.forename: H
authors.initials: H
authors.lastname: Kawai
authors.forename: H
authors.initials: H
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Igarashi
authors.forename: T
authors.initials: T
authors.lastname: Miyazaki
mesh_terms: D016475:3T3 Cells; D000327:Adsorption; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D005347:Fibroblasts / Q000166:cytology; D005353:Fibronectins / Q000737:chemistry; D006851:Hydrochloric Acid / Q000737:chemistry; D006868:Hydrolysis; D006997:Hypochlorous Acid / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000166:cytology; D012965:Sodium Chloride / Q000737:chemistry*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D005353:Fibronectins; C009495:titanium dioxide; D012965:Sodium Chloride; D006997:Hypochlorous Acid; D014025:Titanium; D006851:Hydrochloric Acid; C046804:titanium hydroxide