Application of preparative high-speed counter-current chromatography for separation of chlorogenic acid from Flos Lonicerae.
Chlorogenic acid, an ester formed between caffeic acid and quinic acid, is a major phenolic compound in the traditional Chinese medicinal herb Flos Lonicerae. The separation and purification of chlorogenic acid from the crude extract of Flos Lonicerae was achieved by high-speed counter-current chromatography (HSCCC). A high acid, highly polar two-phase solvent system containing n-butanol-acetic acid-water (4:1:5) was run on a preparative scale. The upper phase was used as the mobile phase in the head to tail elution mode. A 300-mg quantity of the crude extract containing 5.97% chlorogenic acid was loaded on a 342-ml HSCCC column. Double separations were performed with the same solvent system yielding 16.9 mg chlorogenic acid at 94.8% purity with approximately 90% recovery.
Lu
Hai-Tao
HT
Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
Jiang
Yue
Y
Chen
Feng
F
D002726:Chlorogenic Acid / Q000302:isolation & purification*; D003377:Countercurrent Distribution / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D012997:Solvents
D004365:Drugs, Chinese Herbal; D012997:Solvents; D002726:Chlorogenic Acid