title: [Effect of cyclin G1 antisense oligodeoxynucleotides (ASON) on the growth of HL-60 cells in nude mice].
abstract: OBJECTIVE
  To study the inhibition effect of cyclin G(1) antisense oligodeoxynucleotides (ASON) on the growth of HL-60 cells in nude mice.
  
  
  METHODS
  (1) Nude mice were divided into control group, sense oligodeoxynucleotides (SON) group and ASON group. After (60)Co radiation, with HL-60 cells SON group and ASON group were subcutaneously innoculated; (2) The weight and volume of tumors were continually measured; (3) The morphology of tumor cells was observed by microscope; (4) The protein and mRNA expression levels of cyclin G(1) were determined by flow cytometry (FCM) and reverse transcription polymerase chain reaction (RT-PCR); (5) The cell apoptosis was detected by electron microscopy and FCM.
  
  
  RESULTS
  (1) The inhibition rate of tumor in ASON group was 69.4%. In ASON group, the wight and volume of tumor were significantly lower than those in SON group and control group. (2) The HL-60 cells in ASON group showed morphologically smaller nuclei, less mitosis, less heteromorphosis and apoptosis.
  
  
  CONCLUSIONS
  The cyclin G(1) ASON can inhibit the growth of HL-60 cells in nude mice and induce apoptosis.
authors.affiliation: Department of Hematology, the Second Hospital, Hebei Medical University, Shijiazhuang 050000, China.
authors.forename: Jin-song
authors.initials: JS
authors.lastname: Jia
authors.forename: Shi-rong
authors.initials: SR
authors.lastname: Xu
authors.forename: Cun-rong
authors.initials: CR
authors.lastname: Jia
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.forename: Sen
authors.initials: S
authors.lastname: Ha
authors.forename: Yin-rong
authors.initials: YR
authors.lastname: Yao
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Cui-ying
authors.initials: CY
authors.lastname: Shi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D056746:Cyclin G; D056766:Cyclin G1; D016213:Cyclins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D023041:Xenograft Model Antitumor Assays / Q000379:methods
chemical_list: C534702:CCNG1 protein, human; C534703:Ccng1 protein, mouse; D056746:Cyclin G; D056766:Cyclin G1; D016213:Cyclins; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger