Protein kinase D regulates basolateral membrane protein exit from trans-Golgi network.
Protein kinase D (PKD) binds to diacylglycerol (DAG) in the trans-Golgi network (TGN) and is activated by trimeric G-protein subunits beta gamma. This complex then regulates the formation of transport carriers in the TGN that traffic to the plasma membrane in non-polarized cells. Here we report specificity of different PKD isoforms in regulating protein trafficking from the TGN. Kinase-inactive forms of PKD1, PKD2 and PKD3 localize to the TGN in polarized and non-polarized cells. PKD activity is required only for the transport of proteins containing basolateral sorting information, and seems to be cargo specific.
Yeaman
Charles
C
Department of Anatomy and Cell Biology, University of Iowa, Iowa City, IA 52242-1109, USA.
Ayala
M Inmaculada
MI
Wright
Jessica R
JR
Bard
Frederic
F
Bossard
Carine
C
Ang
Agnes
A
Maeda
Yusuke
Y
Seufferlein
Thomas
T
Mellman
Ira
I
Nelson
W James
WJ
Malhotra
Vivek
V
D000818:Animals; D002460:Cell Line; D016764:Cell Polarity; D004075:Diglycerides / Q000378:metabolism; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D000091283:Protein Kinase D2; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D021601:trans-Golgi Network / Q000378:metabolism*
D004075:Diglycerides; D007527:Isoenzymes; D000091283:Protein Kinase D2; D011993:Recombinant Fusion Proteins; D011494:Protein Kinases; C119190:protein kinase C nu; C089212:protein kinase D; D011493:Protein Kinase C