Characterisation of chicken TES and its role in cell spreading and motility.
Previously we identified TES as a candidate tumour suppressor gene that is located at human chromosome 7q31.1. More recently, we and others have shown TES to encode a novel LIM domain protein that localises to focal adhesions. Here, we present the cloning and functional analysis of the chicken orthologue of TES, cTES. The TES proteins are highly conserved between chicken and human, showing 89% identity at the amino acid level. We show that the cTES protein localised at focal adhesions, actin stress fibres, and sites of cell-cell contact, and GST-cTES can pull-down zyxin and actin. To investigate a functional role for cTES, we looked at the effect of its overexpression on cell spreading and cell motility. Cells overexpressing cTES showed increased cell spreading on fibronectin, and decreased cell motility, compared to RCAS vector transfected control cells. The data from our studies with cTES support our previous findings with human TES and further implicate TES as a member of a complex of proteins that function together to regulate cell adhesion and additionally demonstrate a role for TES in cell motility.
Griffith
Elen
E
Cancer Research UK Laboratories, Beatson Institute for Cancer Research, Bearsden, Glasgow, United Kingdom.
Coutts
Amanda S
AS
Black
Donald M
DM
D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins; D002448:Cell Adhesion; D002465:Cell Movement / Q000502:physiology*; D048429:Cell Size / Q000502:physiology*; D002645:Chickens; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics; D003598:Cytoskeletal Proteins; D022001:Focal Adhesions / Q000378:metabolism*; D016147:Genes, Tumor Suppressor / Q000502:physiology*; D006023:Glycoproteins; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D060588:LIM Domain Proteins; D008667:Metalloproteins / Q000378:metabolism; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D016601:RNA-Binding Proteins; D022502:Stress Fibers / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism; D060589:Zyxin
D000199:Actins; D030161:Avian Proteins; D003598:Cytoskeletal Proteins; D006023:Glycoproteins; D018398:Homeodomain Proteins; D060588:LIM Domain Proteins; D008667:Metalloproteins; D016601:RNA-Binding Proteins; C429688:TES protein, human; D025521:Tumor Suppressor Proteins; C092842:ZYX protein, human; D060589:Zyxin