Adipocyte differentiation of multipotent cells established from human adipose tissue.
In this study multipotent adipose-derived stem cells isolated from human adipose tissue (hMADS cells) were shown to differentiate into adipose cells in serum-free, chemically defined medium. During the differentiation process, hMADS cells exhibited a gene expression pattern similar to that described for rodent clonal preadipocytes and human primary preadipocytes. Differentiated cells displayed the key features of human adipocytes, i.e., expression of specific molecular markers, lipolytic response to agonists of beta-adrenoreceptors (beta2-AR agonist > beta1-AR agonist >> beta3-AR agonist) and to the atrial natriuretic peptide, insulin-stimulated glucose transport, and secretion of leptin and adiponectin. hMADS cells were able to respond to drugs as inhibition of adipocyte differentiation was observed in the presence of prostaglandin F2alpha, tumour necrosis factor-alpha, and nordihydroguaiaretic acid, a natural polyhydroxyphenolic antioxidant. Thus, for the first time, human adipose cells with normal karyotype and indefinite life span have been established. They represent a novel and valuable tool for studies of fat tissue development and metabolism.
Rodriguez
Anne-Marie
AM
Institut de Recherche Signalisation, Biologie du Développement et Cancer, UMR 6543 CNRS, Centre de Biochimie, Faculté des Sciences, Parc Valrose, 06108 Nice cedex 2, France.
Elabd
Christian
C
Delteil
Frédéric
F
Astier
Julien
J
Vernochet
Cécile
C
Saint-Marc
Perla
P
Guesnet
Joëlle
J
Guezennec
Anne
A
Amri
Ez-Zoubir
EZ
Dani
Christian
C
Ailhaud
Gérard
G
D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D058665:Adrenergic beta-1 Receptor Agonists; D058666:Adrenergic beta-2 Receptor Agonists; D058667:Adrenergic beta-3 Receptor Agonists; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001692:Biological Transport; D015152:Blotting, Northern; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D017931:DNA Primers / Q000737:chemistry; D015237:Dinoprost / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007621:Karyotyping; D020738:Leptin / Q000378:metabolism; D008297:Male; D009637:Masoprocol / Q000378:metabolism; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D058665:Adrenergic beta-1 Receptor Agonists; D058666:Adrenergic beta-2 Receptor Agonists; D058667:Adrenergic beta-3 Receptor Agonists; D000975:Antioxidants; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D017931:DNA Primers; D020738:Leptin; D014409:Tumor Necrosis Factor-alpha; D009637:Masoprocol; D015237:Dinoprost; D005947:Glucose