TMC-95A, a reversible proteasome inhibitor, induces neurite outgrowth in PC12 cells.
TMC-95A has been characterized as a potent proteasome inhibitor that binds to enzymes non-covalently at low nanomolar concentrations. Herein, the neuritogenic activity of TMC-95A in PC12 rat pheochromocytoma cells is reported for the first time. TMC-95A induced a positive neurite initiation of PC12 cells at concentration ranging from 1 to 20 microM.
Inoue
Masayuki
M
Department of Chemistry, Graduate School of Science, Tohoku University, Sendai 980-8578, Japan. inoue@ykbsc.chem.tohoku.ac.jp
Zhai
Haifeng
H
Sakazaki
Hayato
H
Furuyama
Hidetomo
H
Fukuyama
Yoshiyasu
Y
Hirama
Masahiro
M
D000818:Animals; D001203:Ascomycota / Q000737:chemistry*; D003546:Cysteine Endopeptidases / Q000187:drug effects*; D015394:Molecular Structure; D009097:Multienzyme Complexes / Q000187:drug effects*; D016501:Neurites / Q000187:drug effects* / Q000502:physiology; D016716:PC12 Cells; D010456:Peptides, Cyclic / Q000302:isolation & purification / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D046988:Proteasome Endopeptidase Complex; D051381:Rats
D009097:Multienzyme Complexes; D010456:Peptides, Cyclic; D011480:Protease Inhibitors; C408291:TMC-95A; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex