Subcellular localization of MAK-V/Hunk protein kinase expressed in COS-1 cells.
MAK-V/Hunk is a MARK/Par-1-related protein kinase, whose function is unknown. We studied the subcellular localization of MAK-V/Hunk in COS-1 cells by immunofluorescence. It has a nucleocytoplasmic distribution and is localized to the centrosome, as indicated by co-localization with gamma-tubulin. A putative kinase-deficient mutant, with a mutation in the invariant lysine residue in the catalytic domain, was not targeted to the nucleus or centrosome. These results suggest that the nuclear and centrosomal targeting of MAK-V/Hunk is specific, and is likely to be coupled to its catalytic activity.
Korobko
Elena V
EV
Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov Street, Moscow 119334, Russia.
Kiselev
Sergey L
SL
Korobko
Igor V
IV
D021581:Active Transport, Cell Nucleus; D019943:Amino Acid Substitution; D000818:Animals; D000911:Antibodies, Monoclonal; D019556:COS Cells; D020134:Catalytic Domain; D002460:Cell Line; D018385:Centrosome / Q000737:chemistry / Q000201:enzymology; D002522:Chlorocebus aethiops; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D051379:Mice; D010957:Plasmids; D011494:Protein Kinases / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases
D000911:Antibodies, Monoclonal; D011494:Protein Kinases; C417729:Hunk protein, mouse; D017346:Protein Serine-Threonine Kinases