Membrane properties of single muscle cells of the rhabdosphincter of the male urethra.
BACKGROUND
The electrophysiological properties of myoblast cultures established from the human and porcine rhabdosphincter (RS) and porcine lower limb muscle (LLSKM) were studied to elucidate their potential for tissue engineering applications in the lower urinary tract.


METHODS
Muscle biopsies were collected from the prostatic part of the RS, the RS of male pigs, and the porcine LLSKM. Ion channels were studied by means of the patch-clamp technique.


RESULTS
Only one subtype each of voltage gated Na+ and Ca2+ channels was observed in porcine RS and LLSKM. Two types of voltage gated Ca2+ channels were identified in human RS cells. The porcine RS and LLSKM myoblasts displayed similar fusion competence.


CONCLUSIONS
Porcine RS and LLSKM myoblasts and human RS and human skeletal muscle cells show a high degree of similarity. Injection of autologous skeletal muscle myoboblasts in the lower urinary tract might, therefore, represent a promising approach to treat stress incontinence after radical prostatectomy.
Berjukow
Stanislav
S
Institute for Biochemical Pharmacology, Peter Mayr Strasse 1, Innsbruck, Austria.
Margreiter
Eva
E
Marksteiner
Rainer
R
Strasser
Hannes
H
Bartsch
Georg
G
Hering
Steffen
S
D000818:Animals; D020746:Calcium Channels, L-Type / Q000502:physiology; D020747:Calcium Channels, T-Type / Q000502:physiology; D002455:Cell Division; D002459:Cell Fusion; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D002999:Clone Cells / Q000166:cytology; D004594:Electrophysiology; D006614:Hindlimb; D006801:Humans; D007866:Leg; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology / Q000502:physiology; D032446:Myoblasts / Q000166:cytology* / Q000502:physiology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000502:physiology*; D015222:Sodium Channels / Q000502:physiology; D013552:Swine; D014521:Urethra / Q000166:cytology* / Q000502:physiology*
D020746:Calcium Channels, L-Type; D020747:Calcium Channels, T-Type; D015222:Sodium Channels