Affinity-independent elution of antibody-displaying phages using cleavable DNA linker containing streptavidin beads.
The search for high-affinity antibodies from phage libraries by panning is often complicated by inefficient detachment of the captured phage particles from the antigen-coated solid phase. General elution methods, independent on the bioaffinity interaction, would be desirable for high-throughput antibody screening. We have developed a bioaffinity-independent elution method based on the use of commercially available streptavidin paramagnetic particles.
Santala
Ville
V
Department of Biotechnology, University of Turku, FIN-20520 Turku, Finland. ville.santala@utu.fi
Saviranta
Petri
P
D000906:Antibodies / Q000302:isolation & purification*; D019921:Biotinylation; D007118:Immunoassay; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry; D018189:Immunomagnetic Separation / Q000379:methods*; D019151:Peptide Library; D011994:Recombinant Proteins / Q000302:isolation & purification; D019809:Streptavidin / Q000737:chemistry
D000906:Antibodies; D007140:Immunoglobulin Fab Fragments; D019151:Peptide Library; D011994:Recombinant Proteins; D019809:Streptavidin