title: Expression of acyl-CoA synthetase 5 reflects the state of villus architecture in human small intestine.
abstract: Several disorders of the small intestine are associated with disturbances in villus architecture. Thus, an understanding of the molecular mechanisms associated with the differentiation of villi represents an important step in the improvement of the understanding of small intestinal pathology. Screening of antibodies from a hybridoma library led to the identification of an acyl-CoA synthetase 5-specific monoclonal antibody. Protein synthesis, mRNA expression, and the enzyme activity of acyl-CoA synthetase 5 were studied by several methods in human small intestinal tissues with Crohn's disease or coeliac disease, respectively. Acyl-CoA synthetase 5 mRNA and protein levels were substantially reduced in injured small intestinal mucosa. Moreover, impaired synthesis of the acyl-CoA synthetase 5 protein was reflected by a decrease in intramucosal enzyme activity. Subtle changes of the acyl-CoA synthetase 5 pattern correlate with conversion of intestinal epithelial cells to a gastric phenotype. These results suggest that deranged acyl-CoA synthetase 5 expression, synthesis, and activity are closely related to the state of villus architecture and epithelial homeostasis in human small intestine.
authors.affiliation: Department of Pathology, University of Heidelberg, Heidelberg, Germany. Nikolaus_Gassler@med.uni-heidelberg.de
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Gassler
authors.forename: Jürgen
authors.initials: J
authors.lastname: Kopitz
authors.forename: Arman
authors.initials: A
authors.lastname: Tehrani
authors.forename: Birgit
authors.initials: B
authors.lastname: Ottenwälder
authors.forename: Martina
authors.initials: M
authors.lastname: Schnölzer
authors.forename: Jürgen
authors.initials: J
authors.lastname: Kartenbeck
authors.forename: Stefan
authors.initials: S
authors.lastname: Lyer
authors.forename: Frank
authors.initials: F
authors.lastname: Autschbach
authors.forename: Annemarie
authors.initials: A
authors.lastname: Poustka
authors.forename: Herwart F
authors.initials: HF
authors.lastname: Otto
authors.forename: Jan
authors.initials: J
authors.lastname: Mollenhauer
mesh_terms: D000328:Adult; D000368:Aged; D015153:Blotting, Western; D002446:Celiac Disease / Q000201:enzymology*; D003066:Coenzyme A Ligases / Q000235:genetics / Q000378:metabolism*; D003424:Crohn Disease / Q000201:enzymology*; D015870:Gene Expression; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D007413:Intestinal Mucosa / Q000201:enzymology; D007421:Intestine, Small / Q000201:enzymology*; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D003066:Coenzyme A Ligases; C115795:acyl CoA synthetase 5; C485465:ACSL5 protein, human