title: Colony aggregation and biofilm formation in xylem chemistry-based media for Xylella fastidiosa.
abstract: Two chemically defined media based on xylem fluid chemistry were developed for Xylella fastidiosa. These media were tested and compared to chemically defined media XDM2, XDM4 and XF-26. New media were evaluated for the Pierce's disease (PD) strain UCLA-PD. Our media either was similar to the concentration of some amino acids found in the xylem fluid of the PD-susceptible Vitis vinifera cv. Chardonnay (medium CHARD2) or incorporated the tripeptide glutathione found in xylem fluid composition (medium 3G10-R). CHARD2 and 3G10-R are among the simplest chemically defined media available. Xylem fluid chemistry-based media supported X. fastidiosa growth and especially stimulated aggregation and biofilm formation.
authors.affiliation: North Florida Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, 155 Research Road, Quincy, FL 32351, USA. bleite@mail.ufl.edu
authors.forename: Breno
authors.initials: B
authors.lastname: Leite
authors.forename: Peter Craig
authors.initials: PC
authors.lastname: Andersen
authors.forename: Maria Lucia
authors.initials: ML
authors.lastname: Ishida
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D001422:Bacterial Adhesion; D001431:Bacteriological Techniques; D018441:Biofilms / Q000254:growth & development*; D003470:Culture Media / Q000737:chemistry; D005978:Glutathione / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D027843:Vitis / Q000737:chemistry / Q000382:microbiology; D044167:Xylella / Q000254:growth & development* / Q000502:physiology
chemical_list: D000596:Amino Acids; D003470:Culture Media; D005978:Glutathione