title: Increased pulmonary blood flow does not alter surfactant protein gene expression in lambs within the first week of life.
abstract: Neonates and infants with congenital heart disease with increased pulmonary blood flow suffer morbidity from poor oxygenation and decreased lung compliance. In a previous experiment involving 4-wk-old lambs with pulmonary hypertension secondary to increased pulmonary blood flow following an in utero placement of an aortopulmonary vascular graft, we found a decrease in surfactant protein (SP)-A gene expression as well as a decrease in SP-A and SP-B protein contents. To determine the timing of these changes, the objective of the present study was to characterize the effect of increased pulmonary blood flow and pulmonary hypertension on SP-A, -B, and -C gene expressions and protein contents within the first week of life. Of eight fetal lambs that underwent the in utero placement of the shunt, there was no difference in the expression of SP-A, -B, and -C mRNA levels or SP-A and -B protein contents compared with age-matched controls. The results showed that, in this model of congenital heart disease with pulmonary hypertension and increased pulmonary blood flow, the effect of the shunt on SP gene expression and protein content was not apparent within the first week of life.
authors.affiliation: Department of Anesthesiology, University of California, CA 94143, USA. leejw@anesthesia.ucsf.edu
authors.forename: Jae W
authors.initials: JW
authors.lastname: Lee
authors.forename: Boaz
authors.initials: B
authors.lastname: Ovadia
authors.forename: Anthony
authors.initials: A
authors.lastname: Azakie
authors.forename: Sonia
authors.initials: S
authors.lastname: Salas
authors.forename: Jon
authors.initials: J
authors.lastname: Goerke
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Fineman
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Gutierrez
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D019332:Endothelin-1 / Q000502:physiology; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D009569:Nitric Oxide / Q000502:physiology; D010713:Phosphatidylcholines / Q000378:metabolism; D011247:Pregnancy; D011652:Pulmonary Circulation / Q000502:physiology*; D037662:Pulmonary Surfactant-Associated Protein A / Q000235:genetics; D037701:Pulmonary Surfactant-Associated Protein B / Q000235:genetics; D037721:Pulmonary Surfactant-Associated Protein C / Q000235:genetics; D037661:Pulmonary Surfactant-Associated Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D012756:Sheep; D014655:Vascular Resistance / Q000502:physiology
chemical_list: D019332:Endothelin-1; D010713:Phosphatidylcholines; D037662:Pulmonary Surfactant-Associated Protein A; D037701:Pulmonary Surfactant-Associated Protein B; D037721:Pulmonary Surfactant-Associated Protein C; D037661:Pulmonary Surfactant-Associated Proteins; D012333:RNA, Messenger; D009569:Nitric Oxide