title: Depletion of host reactive T cells by photodynamic cell purging and prevention of graft versus host disease.
abstract: Graft versus Host Disease (GVHD) is the principal cause of morbidity and mortality in patients undergoing allogeneic stem cell transplant. T cell depletion has been recognized as a method of reducing the incidence of GVHD in allogeneic transplants. Until recently, most T cell depletion methods were non-selective in reducing lymphocytes. Rhodamine purging is one method, which selectively reduces alloreactive T cells preventing GVHD. We review here the methods of non-selective and selective T cell depletion, particularly the newer method of photodynamic purging utilizing rhodamine.
authors.affiliation: Hematology-Oncology, Duke University Medical Center, 2400 Pratt Street, Ste. 1100, Durham, NC 27710, USA. goggi002@mc.duke.edu
authors.forename: Timothy F
authors.initials: TF
authors.lastname: Goggins
authors.forename: Nelson
authors.initials: N
authors.lastname: Chao
mesh_terms: D000818:Animals; D006086:Graft vs Host Disease / Q000517:prevention & control*; D020544:Graft vs Leukemia Effect; D006801:Humans; D008212:Lymphocyte Depletion; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000494:pharmacology*; D012235:Rhodamines / Q000494:pharmacology*; D033581:Stem Cell Transplantation; D013601:T-Lymphocytes / Q000187:drug effects*; D014181:Transplantation Immunology; D014184:Transplantation, Homologous
chemical_list: D017319:Photosensitizing Agents; D012235:Rhodamines; C121365:4,5-dibromorhodamine 123