Analyzing protein-protein interactions in cell membranes.
Interactions among membrane proteins regulate numerous cellular processes, including cell growth, cell differentiation and apoptosis. We need to understand which proteins interact, where they interact and to which extent they interact. This article describes a set of novel approaches to measure, on the surface of living cells, the number of clusters of proteins, the number of proteins per cluster, the number of clusters or membrane domains that contain pairs of interacting proteins and the fraction of one protein species that interacts with another protein within these domains. These data can then be interpreted in terms of the function of the protein-protein interactions.
Nohe
Anja
A
Department of Chemistry, The University of Western Ontario, London, ON N6A 5B7, Canada.
Petersen
Nils O
NO
D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D002478:Cells, Cultured; D017261:Glycosylphosphatidylinositols; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008565:Membrane Proteins / Q000378:metabolism*; D025941:Protein Interaction Mapping / Q000379:methods*; D013223:Statistics as Topic
D017261:Glycosylphosphatidylinositols; D008565:Membrane Proteins