title: Protein kinase D regulates basolateral membrane protein exit from trans-Golgi network.
abstract: Protein kinase D (PKD) binds to diacylglycerol (DAG) in the trans-Golgi network (TGN) and is activated by trimeric G-protein subunits beta gamma. This complex then regulates the formation of transport carriers in the TGN that traffic to the plasma membrane in non-polarized cells. Here we report specificity of different PKD isoforms in regulating protein trafficking from the TGN. Kinase-inactive forms of PKD1, PKD2 and PKD3 localize to the TGN in polarized and non-polarized cells. PKD activity is required only for the transport of proteins containing basolateral sorting information, and seems to be cargo specific.
authors.affiliation: Department of Anatomy and Cell Biology, University of Iowa, Iowa City, IA 52242-1109, USA.
authors.forename: Charles
authors.initials: C
authors.lastname: Yeaman
authors.forename: M Inmaculada
authors.initials: MI
authors.lastname: Ayala
authors.forename: Jessica R
authors.initials: JR
authors.lastname: Wright
authors.forename: Frederic
authors.initials: F
authors.lastname: Bard
authors.forename: Carine
authors.initials: C
authors.lastname: Bossard
authors.forename: Agnes
authors.initials: A
authors.lastname: Ang
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Maeda
authors.forename: Thomas
authors.initials: T
authors.lastname: Seufferlein
authors.forename: Ira
authors.initials: I
authors.lastname: Mellman
authors.forename: W James
authors.initials: WJ
authors.lastname: Nelson
authors.forename: Vivek
authors.initials: V
authors.lastname: Malhotra
mesh_terms: D000818:Animals; D002460:Cell Line; D016764:Cell Polarity; D004075:Diglycerides / Q000378:metabolism; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D000091283:Protein Kinase D2; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D021601:trans-Golgi Network / Q000378:metabolism*
chemical_list: D004075:Diglycerides; D007527:Isoenzymes; D000091283:Protein Kinase D2; D011993:Recombinant Fusion Proteins; D011494:Protein Kinases; C119190:protein kinase C nu; C089212:protein kinase D; D011493:Protein Kinase C