A genomewide oscillation in transcription gates DNA replication and cell cycle.
Microarray analysis from a yeast continuous synchrony culture system shows a genomewide oscillation in transcription. Maximums in transcript levels occur at three nearly equally spaced intervals in this approximately 40-min cycle of respiration and reduction. Two temporal clusters (4,679 of 5,329) are maximally expressed during the reductive phase of the cycle, whereas a third cluster (650) is maximally expressed during the respiratory phase. Transcription is organized functionally into redox-state superclusters with genes known to be important in respiration or reduction being synthesized in opposite phases of the cycle. The transcriptional cycle gates synchronous bursts in DNA replication in a constant fraction of the population at 40-min intervals. Restriction of DNA synthesis to the reductive phase of the cycle may be an evolutionarily important mechanism for reducing oxidative damage to DNA during replication.
Klevecz
Robert R
RR
Dynamics Group, Department of Biology, Beckman Research Institute of the City of Hope Medical Center, Duarte, CA 91010, USA.
Bolen
James
J
Forrest
Gerald
G
Murray
Douglas B
DB
D015152:Blotting, Northern; D002453:Cell Cycle; D004261:DNA Replication; D005434:Flow Cytometry; D016681:Genome, Fungal; D020411:Oligonucleotide Array Sequence Analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic