Cellular accumulation of dietary anticarcinogenic isothiocyanates is followed by transporter-mediated export as dithiocarbamates.
Many dietary isothiocyanates (ITCs) are potent anticarcinogenic agents. ITCs rapidly accumulate to high concentrations in cells as a result of conjugation with intracellular thiols, especially glutathione (GSH). The anticarcinogenic activity of ITCs depends on, at least partly, their accumulation in cells. We report that three major anticarcinogenic ITCs, including allyl-ITC, benzyl-ITC, and phenethyl-ITC, were rapidly exported, upon accumulation in cells, mainly in the forms of GSH- and cysteinylglycine-conjugates, apparently involving MRP-1 and Pgp-1. These findings are consistent with our previous results regarding cellular export of another anticarcinogenic ITC, sulforaphane, and suggest a common cellular response to ITCs.
Callaway
Eileen C
EC
Arizona Cancer Center, College of Medicine, University of Arizona, Tucson, AZ 85724, USA.
Zhang
Yuesheng
Y
Chew
Wade
W
Chow
H-H Sherry
HH
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D016588:Anticarcinogenic Agents / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D018922:HL-60 Cells / Q000378:metabolism; D006801:Humans; D017879:Isothiocyanates / Q000378:metabolism*; D021381:Protein Transport; D013859:Thiocarbamates / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D016588:Anticarcinogenic Agents; D017879:Isothiocyanates; D013859:Thiocarbamates; D005982:Glutathione Transferase; D005978:Glutathione