Expression and significance of VEGF-C and FLT-4 in gastric cancer.
OBJECTIVE
To investigate the expression of pathological factors of VEGF-C and its receptor FLT-4 in primary gastric cancer and adjacent normal tissues.


METHODS
The expression of VEGF-C and FLT-4 was studied in 80 primary gastric cancers and adjacent normal tissues from the same patients by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and immumohistochemistry.


RESULTS
Both primary gastric cancer and adjacent normal tissue could express VEGF-C and FLT-4, and FLT-4 expression was also detected in endothelial cells of stromal blood vessels and lymphatic vessels. There was a significant difference in expression of VEGF-C between primary tumor and adjacent normal tissue samples (P=0.01), and a statistical correlation between VEGF-C and FLT-4 expression in tumors (P=0.00886). With regard to VEGF-C expression, there was a significant difference between moderate-poor differential type and high differential type (P=0.032), and a significant difference between positive and negative lymph node metastases (P=0.024). However, there was no significant difference between positive and negative serosal invasions (P=0.219).


CONCLUSIONS
VEGF-C and its receptor FLT-4 play a role in the development of gastric cancer, and the tumors with expression of VEGF-C and FLT-4 are more likely to have lymph node metastasis.
Liu
Xing-E
XE
Center of Oncology, the Affiliated Sir Run Run Shaw Hospital, Medical College, Zhejiang University, Hangzhou 310016, Zhejiang Province, China. xingel001@yahoo.com
Sun
Xiao-Dong
XD
Wu
Jin-Min
JM
D000328:Adult; D000368:Aged; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D042582:Vascular Endothelial Growth Factor C / Q000378:metabolism*; D040321:Vascular Endothelial Growth Factor Receptor-3 / Q000378:metabolism*
D042582:Vascular Endothelial Growth Factor C; D040321:Vascular Endothelial Growth Factor Receptor-3