[Co-detection of P21, P53 and HSP70 and their possible role in diagnosis of polycyclic aromatic hydrocarbons (PAHs)-related lung cancer].
OBJECTIVE
To explore the biomarkers of early diagnosis in patients with polycyclic aromatic hydrocarbons (PAHs)-related lung cancer for the application to detection of occupational lung cancer or related lung cancer.


METHODS
Western dot blotting was used to explore the expression of ras, p53 and heat stress protein 70 (HSP70) in 29 patients with PAHs-related lung cancer (LC), and 28 patients with non-cancerous pulmonary disease, and 30 healthy controls.


RESULTS
The positive detection rates of P21, P53, and HSP70 in LC group (58.62%, 34.48%, 41.38% respectively) were higher than those in non-cancerous pulmonary disease group (14.29%, 7.14%, 10.71% respectively, P < 0.01). The sensitivity of P21, P53 and HSP70 were 58.62%, 34.48% and 41.38% respectively, negative predictive value (NPV) were 68.42%, 78.05% and 63.04% respectively. The co-detection of the three proteins mentioned above produced a sensitivity of 82.76% with a NPV of 78.26% (P < 0.05). Of 18 cases of LC with negative cytology, 13 (72.22%) were found HSP21, P53 or HSP70 positive.


CONCLUSIONS
Co-detection of the P21, P53, and HSP70 may be used as the screening marker for diagnosis of PAHs-related lung cancer, and may supplement the diagnostic value of conventional cytology.
Lu
Qiao-fa
QF
Department of Internal Medicine, Wuhan No. 3 Hospital, Wuhan, 430060, China.
Bai
Ming
M
Zhang
Huan-jing
HJ
Li
Ji-chao
JC
Xiao
Cheng-feng
CF
Chen
Sheng
S
Wu
Tang-chun
TC
D000368:Aged; D015415:Biomarkers / Q000032:analysis*; D015153:Blotting, Western; D016022:Case-Control Studies; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis; D006801:Humans; D008175:Lung Neoplasms / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D008875:Middle Aged; D016273:Occupational Exposure; D011084:Polycyclic Aromatic Hydrocarbons / Q000506:poisoning*; D016283:Proto-Oncogene Proteins p21(ras) / Q000032:analysis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D011084:Polycyclic Aromatic Hydrocarbons; D016159:Tumor Suppressor Protein p53; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)