title: Rapid determination of benzo(a)pyrene in olive oil samples with solid-phase extraction and low-pressure, wide-bore gas chromatography-mass spectrometry and fast liquid chromatography with fluorescence detection.
abstract: Benzo(a)pyrene (B(a)P) was extracted from olive oil using solid-phase extraction on columns filled with Florisil and Nucleoprep C18. The extracts were analyzed with GC-MS, using standard capillary column and low-pressure wide-bore column (LP-GC-MS), as well as with HPLC on standard column and short donor-acceptor complex chromatography (DACC) column. Quantitation was done with isotope dilution method (GC-MS and LP-GC-MS) or with internal standard benzo(k)fluoranthene (HPLC). Limits of detection were 1 ng/g for GC-MS on standard column, 1.6 ng/g on LP-column, 0.5 ng/g for HPLC on standard column, and 0.3 ng/g on DACC column, respectively. The applied extraction method allowed handling over 50 samples per day and assured recovery over 80%. Matrix solid-phase dispersion, tried as an alternative isolation method, appeared less advantageous. Fast chromatographic methods (LP-GC-MS and HPLC on DACC) made it possible to reduce analysis time to 8 and 5 min, respectively. The method was applied to routine analysis of B(a)P in olive oil samples.
authors.affiliation: Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Centre, PO Box 3354, MBC 10, 11211 Riyadh, Saudi Arabia. mbogusz@web.de
authors.forename: Maciej J
authors.initials: MJ
authors.lastname: Bogusz
authors.forename: Samir Abu
authors.initials: SA
authors.lastname: El Hajj
authors.forename: Zeyad
authors.initials: Z
authors.lastname: Ehaideb
authors.forename: Huda
authors.initials: H
authors.lastname: Hassan
authors.forename: Mohammad
authors.initials: M
authors.lastname: Al-Tufail
mesh_terms: D001564:Benzo(a)pyrene / Q000032:analysis* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005453:Fluorescence; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry*; D011312:Pressure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000069463:Olive Oil; D010938:Plant Oils; D001564:Benzo(a)pyrene