Comparative pharmacokinetic behavior of glycyrrhetic acid after oral administration of glycyrrhizic acid and Gancao-Fuzi-Tang.
Comparative pharmacokinetic profiles of glycyrrhetic acid (GA), glycyrrhizic acid (GL) and Gancao-Fuzi-Tang (KF) after oral administration of GL and KF were studied. Plasma samples taken from rats were acidified with acetic acid and GA was extracted with isopropanol-ethyl ether (1 : 1). Separation of GA was performed on a C(18) column with the detection wavelength set at 254 nm. The mobile phase was methanol-acetonitrile-water-acetic acid (58 : 18 : 24 : 1 v/v). The results showed that the mean residence time and area under the curve of GA in KF-administered rats were 27.6+/-1.5 h and 122.8+/-46.7 microg.h/ml respectively, which were significantly different from those in GL-administered rats (15.0+/-2.0 h and 40.9+/-9.6 microg.h/ml, respectively). The results suggest the increased effect of GA after oral administration of KF in comparison with GL.
Gao
Qiu-Tao
QT
School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110-016, Liaoning Province, P.R. China.
Chen
Xiao-Hui
XH
Bi
Kai-Shun
KS
D031841:Aconitum / Q000737:chemistry; D000284:Administration, Oral; D000818:Animals; D031174:Atractylodes / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D032904:Cinnamomum aromaticum / Q000737:chemistry; D004347:Drug Interactions; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics / Q000494:pharmacology; D006034:Glycyrrhetinic Acid / Q000097:blood / Q000493:pharmacokinetics*; D029882:Glycyrrhiza uralensis / Q000737:chemistry; D019695:Glycyrrhizic Acid / Q000097:blood / Q000493:pharmacokinetics* / Q000494:pharmacology; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; D019695:Glycyrrhizic Acid; D006034:Glycyrrhetinic Acid