title: Proteome analysis for the identification of tumor-associated biomarkers in gastrointestinal cancer.
abstract: Gastrointestinal cancers are usually diagnosed at advanced stages, making a curative treatment difficult. Biomarkers can help to overcome this problem by allowing earlier diagnosis, and thus better therapy. Proteomics tools are novel technologies to identify such biomarkers. This review summarizes advances in biomarker detection using two-dimensional gel electrophoresis (2D-PAGE), chromatography and mass spectrometry technologies. 2D-PAGE combined with mass spectrometry has led to the identification of several differentially expressed proteins in cancer tissue. However, for serum analysis, 2D-PAGE has severe limitations. For serum-based cancer diagnosis, surface-enhanced laser desorption-ionization time-of-flight (SELDI-TOF) mass spectrometry is a promising new technology. The potential of proteins identified with this technology as novel cancer biomarkers still needs to be confirmed in clinical trials.
authors.affiliation: Europroteome AG, Berlin-Hennigsdorf, Germany. lfels@europroteome.com
authors.forename: Lüder M
authors.initials: LM
authors.lastname: Fels
authors.forename: Thomas
authors.initials: T
authors.lastname: Buschmann
authors.forename: Jörn
authors.initials: J
authors.lastname: Meuer
authors.forename: Marc A
authors.initials: MA
authors.lastname: Reymond
authors.forename: Stephanie
authors.initials: S
authors.lastname: Lamer
authors.forename: Christoph
authors.initials: C
authors.lastname: Röcken
authors.forename: Matthias P A
authors.initials: MP
authors.lastname: Ebert
mesh_terms: D014408:Biomarkers, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D005770:Gastrointestinal Neoplasms / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D009363:Neoplasm Proteins / Q000032:analysis*; D040081:Protein Array Analysis; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins