title: Quantification of risperidone and 9-hydroxyrisperidone in plasma and saliva from adult and pediatric patients by liquid chromatography-mass spectrometry.
abstract: A robust and validated LC-MS-MS quantitative method, using column switching and mutiple reaction monitoring was developed for the analysis of risperidone (RIS) and 9-hydroxyrisperidone in human plasma and saliva. The analytical range was 1-100 ng/ml. The method used 25 microl of sample precipitated with 75 microl of acetonitrile containing internal standard (R068808). Analyses were conducted on a PE Sciex API-III + triple quadrupole mass spectrometer fitted with a Turbo IonSpray source. The method was validated for human plasma using EDTA as the anticoagulant and cross-validated to heparinized human plasma and saliva. The recoveries of risperidone and 9-hydroxyrisperidone were 90-93 and 89-93%, respectively. The validated method was applied to clinical samples to study risperidone and 9-hydroxyrisperidone concentrations in plasma and saliva. Risperidone and 9-hydroxyrisperidone appear in the saliva of patients treated with risperidone. Their detection/quantification in saliva provides evidence for recent adherence with therapy.
authors.affiliation: Department of Chemistry and Chemical Biology, Barnett Institute, Northeastern University, 360 Huntington Avenue, 102 Hurtig Hall, Boston, MA 02115, USA. flarakosj@earthlink.net
authors.forename: J
authors.initials: J
authors.lastname: Flarakos
authors.forename: W
authors.initials: W
authors.lastname: Luo
authors.forename: M
authors.initials: M
authors.lastname: Aman
authors.forename: D
authors.initials: D
authors.lastname: Svinarov
authors.forename: N
authors.initials: N
authors.lastname: Gerber
authors.forename: P
authors.initials: P
authors.lastname: Vouros
mesh_terms: D000328:Adult; D002138:Calibration; D011232:Chemical Precipitation; D002648:Child; D002853:Chromatography, Liquid; D006801:Humans; D007555:Isoxazoles / Q000032:analysis* / Q000097:blood*; D013058:Mass Spectrometry; D015394:Molecular Structure; D000068882:Paliperidone Palmitate; D011743:Pyrimidines / Q000032:analysis* / Q000097:blood*; D015203:Reproducibility of Results; D018967:Risperidone / Q000032:analysis* / Q000097:blood*; D012463:Saliva / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D007555:Isoxazoles; D011743:Pyrimidines; D018967:Risperidone; D000068882:Paliperidone Palmitate