title: Biotin enhances ATP synthesis in pancreatic islets of the rat, resulting in reinforcement of glucose-induced insulin secretion.
abstract: Previous studies showed that biotin enhanced glucose-induced insulin secretion. Changes in the cytosolic ATP/ADP ratio in the pancreatic islets participate in the regulation of insulin secretion by glucose. In the present study we investigated whether biotin regulates the cytosolic ATP/ADP ratio in glucose-stimulated islets. When islets were stimulated with glucose plus biotin, the ATP/ADP ratio increased to approximately 160% of the ATP/ADP ratio in islets stimulated with glucose alone. The rate of glucose oxidation, assessed by CO(2) production, was also about 2-fold higher in islets treated with biotin. These increasing effects of biotin were proportional to the effects seen in insulin secretion. There are no previous reports of vitamins, such as biotin, directly affecting ATP synthesis. Our data indicate that biotin enhances ATP synthesis in islets following the increased rate of substrate oxidation in mitochondria and that, as a consequence of these events, glucose-induced insulin release is reinforced by biotin.
authors.affiliation: High Technology Research Center, Kagawa Nutrition University, 3-9-21 Chiyoda, Sakado, 350-0288, Saitama, Japan. hsone@eiyo.ac.jp
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Sone
authors.forename: Yuka
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Michio
authors.initials: M
authors.lastname: Komai
authors.forename: Masaaki
authors.initials: M
authors.lastname: Toyomizu
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Kagawa
authors.forename: Yuji
authors.initials: Y
authors.lastname: Furukawa
mesh_terms: D000244:Adenosine Diphosphate / Q000096:biosynthesis; D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives / Q000494:pharmacology*; D003600:Cytosol / Q000378:metabolism; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D008293:Malates / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism; D010084:Oxidation-Reduction; D019289:Pyruvic Acid / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012119:Respiration / Q000187:drug effects
chemical_list: D007328:Insulin; D008293:Malates; D000244:Adenosine Diphosphate; D001710:Biotin; C030298:malic acid; D019289:Pyruvic Acid; D000255:Adenosine Triphosphate; D005947:Glucose