title: On-chip electrochemical measurement of beta-galactosidase expression using a microbial chip.
abstract: [small beta]-Galactosidase expression in a small number of Escherichia coli cells has been measured in real time with an electrochemical sensor chip. E. coli cells were embedded using collagen gel within a micropore which was microfabricated onto a chip. The activity of the expressed [small beta]-galactosidase was determined using p-aminophenyl [small beta]-d-galactopyranoside (PAPG) as a substrate.
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai, Japan.
authors.forename: Takatoshi
authors.initials: T
authors.lastname: Kaya
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Nagamine
authors.forename: Nobuto
authors.initials: N
authors.lastname: Matsui
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Yasukawa
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shiku
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Matsue
mesh_terms: D000627:Aminophenols / Q000737:chemistry; D003094:Collagen; D004563:Electrochemistry / Q000379:methods*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005697:Galactosides / Q000737:chemistry / Q000378:metabolism; D005782:Gels; D017930:Genes, Reporter; D008838:Microcomputers; D011809:Quinones / Q000737:chemistry; D013997:Time Factors; D001616:beta-Galactosidase / Q000032:analysis* / Q000235:genetics / Q000378:metabolism
chemical_list: D000627:Aminophenols; D005697:Galactosides; D005782:Gels; D011809:Quinones; C116897:iminoquinone; C010677:4-aminophenyl-beta-galactoside; D003094:Collagen; D001616:beta-Galactosidase