Comparison of ELISA for the detection of porcine serum antibodies to non-structural proteins of foot-and-mouth disease virus.
Three foot-and-mouth disease virus non-structural protein antibody detection kits, CHEKIT FMD-3ABC, UBI FMD NS EIA and DVIVR NSP ELISA, were compared in the study. The results showed that the specificity of the kits ranged from 96.7 to 100% in nai;ve pigs and from 93.6 to 98.1% in vaccinated pigs, and that the DVIVR kit had the highest analytical sensitivity. The kappa statistics for the detection of 612 sera were 0.582, 0.447 and 0.658 for CHEKIT/UBI, CHEKIT/DVIVR and UBI/DVIVR, respectively. This study also revealed that measurable non-structural protein specific antibodies in some of infected pigs were sustained either for shorter periods or in intermittent patterns, thus aggravating the difficulties associated with the removal of pre-exposed pigs in the field.
Lee
Fan
F
Department of Hog Cholera Research, Animal Health Research institute, 376 Chung Cheng Road, Tamsui, Taipei County (251), Taiwan. fanlee@mail.nvri.gov.tw
Lin
Yeou-Liang
YL
Jong
Ming-Hwa
MH
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005536:Foot-and-Mouth Disease / Q000175:diagnosis*; D029544:Foot-and-Mouth Disease Virus / Q000145:classification / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D013552:Swine / Q000821:virology*; D017361:Viral Nonstructural Proteins / Q000032:analysis*
D000914:Antibodies, Viral; D011933:Reagent Kits, Diagnostic; D017361:Viral Nonstructural Proteins