title: Applicability of discovery science approach to determine biological effects of mobile phone radiation.
abstract: We argue that the use of high-throughput screening techniques, although expensive and laborious, is justified and necessary in studies that examine biological effects of mobile phone radiation. The "case of hsp27 protein" presented here suggests that even proteins with only modestly altered (by exposure to mobile phone radiation) expression and activity might have an impact on cell physiology. However, this short communication does not attempt to present the full scientific evidence that is far too large to be presented in a single article and that is being prepared for publication in three separate research articles. Examples of the experimental evidence presented here were designed to show the flow of experimental process demonstrating that the use of high-throughput screening techniques might help in rapid identification of the responding proteins. This, in turn, can help in speeding up of the process of determining whether these changes might affect human health.*
authors.affiliation: Bio-NIR Research Group at the Radiobiology Laboratory, STUK - Radiation and Nuclear Safety Authority, Helsinki, Finland. dariusz.leszczynski@stuk.fi
authors.forename: Dariusz
authors.initials: D
authors.lastname: Leszczynski
authors.forename: Reetta
authors.initials: R
authors.lastname: Nylund
authors.forename: Sakari
authors.initials: S
authors.lastname: Joenväärä
authors.forename: Jukka
authors.initials: J
authors.lastname: Reivinen
mesh_terms: D040421:Cell Phone; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000032:analysis* / Q000378:metabolism; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D004574:Electromagnetic Fields / Q000009:adverse effects*; D015180:Electrophoresis, Gel, Two-Dimensional; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000528:radiation effects; D008856:Microscopy, Fluorescence; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000032:analysis / Q000378:metabolism*; D011725:Pyridines / Q000494:pharmacology; D048051:p38 Mitogen-Activated Protein Kinases
chemical_list: D003598:Cytoskeletal Proteins; D004791:Enzyme Inhibitors; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D007093:Imidazoles; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; D011725:Pyridines; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580