title: Cloning and characterization of the promoter region of the human prostaglandin F2alpha receptor gene.
abstract: The promoter region of the human prostaglandin F2alpha receptor (FP) gene was isolated, sequenced, and characterized. The 5'-flanking region has minimal homology to the bovine, mouse, and rat FP promoters with the exception of a 100-bp region. The human promoter similarly lacks a canonical TATA-box and a CAAT-box. Potential binding sites for SP-1, GATA-1, STAT-1, and AP-1 are present in the 5'-flanking region. One major transcription start site was identified using 5' RLM-RACE analysis and mapped to an adenine residue 262 nucleotides upstream from the initiator codon in exon 2. Transfection of HeLa cells with FP promoter-GFP deletion constructs indicates that the -2437/-1946 region contains repressor activity. DNase I footprinting analysis of this region identifies a footprint over the GATA-like site at -2400. This suggests repression of basal FP transcription may be mediated by a GATA binding site.
authors.affiliation: Perinatal Research Centre, CIHR Group in Perinatal Health and Disease, Department of Obstetrics and Gynecology, University of Alberta, Edmonton, AB T6G 2S2, Canada. dean.zaragoza@ualberta.ca
authors.forename: Dean B
authors.initials: DB
authors.lastname: Zaragoza
authors.forename: Robyn
authors.initials: R
authors.lastname: Wilson
authors.forename: Kathleen
authors.initials: K
authors.lastname: Eyster
authors.forename: David M
authors.initials: DM
authors.lastname: Olson
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D018983:DNA Footprinting; D006367:HeLa Cells; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011982:Receptors, Prostaglandin / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D024363:Transcription Initiation Site
chemical_list: D011982:Receptors, Prostaglandin; C034671:prostaglandin F2alpha receptor