title: E. Coli sepsis induces profound mechanoenergetic inefficiency in the porcine left ventricle.
abstract: Myocardial dysfunction is believed to be a central part of septic multiorgan manifestations. The aim of the present study was to assess whether E. coli sepsis in an in vivo model would induce a dysfunction in the relationship between mechanical work and energy consumption in the left ventricle (LV). Accordingly, we measured hemodynamics, left ventricular pressure-volume area (PVA), and myocardial oxygen consumption (MVo2) in deeply anesthetized pigs. Eight pigs received 2.0 +/- 0.5 x 10(9) E. coli bacteria intravenously, and seven served as controls. Compared with baseline and the control group, no alternations were observed in LV diastolic function or indices of contractility in the septic group. The MVo2-PVA relationship was highly linear in both groups (all r2 = 0.96-0.99). At 5 h, the y-axis intercept of the MVo2-PVA relationship (nonmechanical MVo2) had increased in the sepsis group by 70% compared with baseline (P = 0.004) and by 60% compared with the control group (P = 0.003). Contractile efficiency (the inverse of the MVo2-PVA slope) remained unchanged over time and between groups. The study demonstrates a profound increase in nonmechanical oxygen consumption during E. coli sepsis in the LV.
authors.affiliation: Surgical Research Laboratory, Institute of Clinical Medicine, University of Tromsø, N-9038 Tromsø, Norway. ebrahim.aghajani@fagmed.uit.no
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Aghajani
authors.forename: Christian
authors.initials: C
authors.lastname: Korvald
authors.forename: Dag
authors.initials: D
authors.lastname: Nordhaug
authors.forename: George
authors.initials: G
authors.lastname: Sager
authors.forename: Arthur
authors.initials: A
authors.lastname: Revhaug
authors.forename: Truls
authors.initials: T
authors.lastname: Myrmel
mesh_terms: D000818:Animals; D001794:Blood Pressure; D002395:Catecholamines / Q000378:metabolism; D003971:Diastole; D004926:Escherichia coli / Q000378:metabolism; D004927:Escherichia coli Infections / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006352:Heart Ventricles / Q000382:microbiology* / Q000473:pathology*; D007773:Lactates / Q000378:metabolism; D008297:Male; D009200:Myocardial Contraction; D009206:Myocardium / Q000473:pathology; D010101:Oxygen Consumption; D011312:Pressure; D018805:Sepsis / Q000473:pathology*; D013552:Swine; D013696:Temperature; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D016277:Ventricular Function, Left
chemical_list: D002395:Catecholamines; D005230:Fatty Acids, Nonesterified; D007773:Lactates; D014409:Tumor Necrosis Factor-alpha; D005947:Glucose