Apoptosis, blood-brain barrier, and subarachnoid hemorrhage.
This study was undertaken to investigate the role of apoptosis in the integrity of blood-brain barrier (BBB) in subarachnoid hemorrhage (SAH). BBB permeability changes were examined and found increased on day 7 in a double hemorrhage rat model using Evans blue dye. The BBB permeability increase is coincidental to brain microvascular endothelial cell apoptosis (expression of caspase-8 and -9) occurring on Day 7. However, caspase-8 and caspase-9 inhibitors failed to protect the BBB. Considering that treatment did not completely inhibit apoptosis in brain microvascular endothelial cells, higher doses, earlier and/or multiple applications, and, possibly, more potent caspase inhibitors may be needed.
Gules
I
I
Department of Neurosurgery, University of Mississippi Medical Center, Jackson, Mississippi, USA.
Satoh
M
M
Nanda
A
A
Zhang
J H
JH
D000818:Animals; D017209:Apoptosis; D001812:Blood-Brain Barrier; D002199:Capillary Permeability; D004730:Endothelium, Vascular / Q000473:pathology / Q000503:physiopathology; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D013345:Subarachnoid Hemorrhage / Q000473:pathology / Q000503:physiopathology*