Liquid chromatographic separation of the enantiomers of trans-chlordane, cis-chlordane, heptachlor, heptachlor epoxide and alpha-hexachlorocyclohexane with application to small-scale preparative separation.
Analytical high-performance liquid chromatographic separations of the individual enantiomers of five polychlorinated compounds were obtained on polysaccharide stereoselective HPLC columns. The enantiomers of the pesticides trans-chlordane, cis-chlordane and heptachlor were separated on CHIRALCEL OD using a hexane mobile phase. The enantiomers of the heptachlor metabolite, heptachlor epoxide, were separated on CHIRALPAK AD using a methanol mobile phase. The enantiomers of alpha-hexachlorocyclohexane (alpha-HCH), were separated on CHIRALCEL OJ using a hexane/2-propanol mobile phase. Similar chromatographic conditions using preparative columns were used to isolate approximately 250 mg of each of the individual enantiomers. The purified individual enantiomers have been submitted for testing of their endocrine disruptor (ED) activity.
Champion
William L
WL
Chiral Technologies Inc., 730 Springdale Dr., P.O. Box 564, Exton, PA 19341, USA. bill.champion@us.rhodia.com
Lee
James
J
Garrison
A Wayne
AW
DiMarco
Jennifer C
JC
Matabe
Aki
A
Prickett
Keith B
KB
D002706:Chlordan / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006533:Heptachlor / Q000302:isolation & purification*; D006534:Heptachlor Epoxide / Q000302:isolation & purification*; D001556:Hexachlorocyclohexane / Q000302:isolation & purification*; D007536:Isomerism
D006534:Heptachlor Epoxide; D002706:Chlordan; C040534:alpha-hexachlorocyclohexane; D001556:Hexachlorocyclohexane; D006533:Heptachlor