Protein-protein interaction networks: from interactions to networks.
The goal of interaction proteomics that studies the protein-protein interactions of all expressed proteins is to understand biological processes that are strictly regulated by these interactions. The availability of entire genome sequences of many organisms and high-throughput analysis tools has led scientists to study the entire proteome (Pandey and Mann, 2000). There are various high-throughput methods for detecting protein interactions such as yeast two-hybrid approach and mass spectrometry to produce vast amounts of data that can be utilized to decipher protein functions in complicated biological networks. In this review, we discuss recent developments in analytical methods for large-scale protein interactions and the future direction of interaction proteomics.
Cho
Sayeon
S
Laboratory of Proteome Analysis, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yusong, Daejeon 305-600, South Korea. scho@kribb.re.kr
Park
Sung Goo
SG
Lee
Do Hee
DH
Park
Byoung Chul
BC
D000818:Animals; D019295:Computational Biology; D005544:Forecasting; D015894:Genome, Human; D023281:Genomics; D006801:Humans; D013058:Mass Spectrometry; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D020543:Proteome / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
D011506:Proteins; D020543:Proteome