title: Molecular cross-talk between the transcription, translation, and nonsense-mediated decay machineries.
abstract: It is widely believed that translation occurs only in the cytoplasm of eukaryotes, but recent results suggest some takes place in nuclei, coupled to transcription. Support for this heterodoxy comes from studies of the nonsense-mediated decay (NMD) pathway; this pathway probably uses ribosomes to proofread messenger RNAs. We find components of the machineries involved in transcription, translation and NMD colocalise, interact and copurify, and that interactions between them are probably mediated by the C-terminal domain of the catalytic subunit of RNA polymerase II. These results are simply explained if the NMD machinery uses nuclear ribosomes to translate - and so proofread - newly made transcripts; then, faulty transcripts and any truncated peptides produced by nuclear translation would be degraded.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Iborra
authors.forename: Alexandre E
authors.initials: AE
authors.lastname: Escargueil
authors.forename: Kon Y
authors.initials: KY
authors.lastname: Kwek
authors.forename: Alexandre
authors.initials: A
authors.lastname: Akoulitchev
authors.forename: Peter R
authors.initials: PR
authors.lastname: Cook
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D018801:CD2 Antigens / Q000235:genetics / Q000378:metabolism; D019556:COS Cells; D020134:Catalytic Domain / Q000502:physiology; D002467:Cell Nucleus / Q000378:metabolism; D018389:Codon, Nonsense / Q000378:metabolism*; D006224:Cricetinae; D003593:Cytoplasm / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D028884:Karyopherins / Q000037:antagonists & inhibitors / Q000378:metabolism; D008954:Models, Biological; D014176:Protein Biosynthesis / Q000502:physiology*; D012319:RNA Polymerase II / Q000302:isolation & purification / Q000378:metabolism*; D020871:RNA Stability / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic / Q000502:physiology*; D000097606:Exportin 1 Protein
chemical_list: D000903:Antibiotics, Antineoplastic; D018801:CD2 Antigens; D018389:Codon, Nonsense; D005231:Fatty Acids, Unsaturated; D028884:Karyopherins; D012319:RNA Polymerase II; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D011994:Recombinant Proteins; D014157:Transcription Factors; D000097606:Exportin 1 Protein; C038753:leptomycin B