Isolation and characterization of resident macrophages from the smooth muscle layers of murine small intestine.
Macrophages within the murine tunica muscularis were isolated and cultured for physiological studies. Following dispersion, macrophages were identified by phagocytotic activity of fluorescein isothiocyanate (FITC)-dextran. Immediately following isolation, macrophages were rounded and possessed fluorescent granula but developed a ramified shape after 3-4 days in culture. Resident and cultured macrophages were immunopositive for F4/80 and I-Ad/I-Ed. Greater than 90% of F4/80 positive cultured cells were FITC-dextran positive. Macrophages had resting membrane potentials (RMP) of -33.3 +/- 1.5 mV after 1 day in culture, which increased to -53.9 +/- 4.4 mV after 3-4 days. The change in RMP was associated with the development of an inward rectifying K+ current, and a decrease in a voltage-dependent, inactivating outward current. After 3-4 days in culture the inflammatory mediated substances adenosine triphosphate (ATP), platelet-activating factor and bacterial lipopolysaccharide induced increases in cytoplasmic Ca2+ ([Ca2+]i). Forskolin suppressed the ATP-induced increase in [Ca2+]i. Macrophages exhibited oxidative bursts, measured by oxidation of dihydrorhodamine-123 to rhodamine-123. Oxidative bursts coincided with a reduction in intracellular pH. Macrophages expressed a proton conductance that may participate in pH maintenance during reactive oxygen production. These results suggest that resident macrophages in the intestine may play a role in the immunological protection of the gut.
Ozaki
H
H
Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo, Japan. aozaki@mail.ecc.u-tokyo.ac.jp
Kawai
T
T
Shuttleworth
C W
CW
Won
K-J
KJ
Suzuki
T
T
Sato
K
K
Horiguchi
H
H
Hori
M
M
Karaki
H
H
Torihashi
S
S
Ward
S M
SM
Sanders
K M
KM
D000818:Animals; D002118:Calcium / Q000032:analysis; D002478:Cells, Cultured; D006863:Hydrogen-Ion Concentration; D007421:Intestine, Small / Q000166:cytology / Q000276:immunology*; D007424:Intracellular Fluid / Q000737:chemistry; D008264:Macrophages / Q000166:cytology / Q000502:physiology*; D008564:Membrane Potentials; D051379:Mice; D009130:Muscle, Smooth / Q000166:cytology / Q000276:immunology*; D018408:Patch-Clamp Techniques; D016897:Respiratory Burst / Q000502:physiology
D002118:Calcium