title: A simple and efficient method for testing Lettuce mosaic virus resistance in in vitro cultivated lettuce.
abstract: The potential of a new in vitro inoculation and propagation method developed for Lettuce mosaic virus (LMV) on lettuce (Lactuca sativa L.) was evaluated by studying LMV infection on in vitro cultivated seedlings or on newly regenerated plantlets. Lettuce cultivars differing by their LMV-resistance status were inoculated with various natural LMV isolates as well as with Green Fluorescent Protein (GFP)-tagged recombinant virus isolates. A good correlation was observed between the known resistance status of the cultivars and the results obtained by in vitro screening. The results show that this resistance assay can be greatly improved by the use of GFP-tagged virus isolates. The main advantages of this method are reduced space requirements and an improved environmental safety, especially for the handling of recombinant virus, of quarantine virus or of transgenic plants.
authors.affiliation: Unité de Génétique et d'Amélioration des Fruits et Légumes, INRA, Domaine Saint Maurice, BP94, 84143 Montfavet Cedex, France. mazier@avignon.inra.fr
authors.forename: Marianne
authors.initials: M
authors.lastname: Mazier
authors.forename: Sylvie
authors.initials: S
authors.lastname: German-Retana
authors.forename: Fabrice
authors.initials: F
authors.lastname: Flamain
authors.forename: Vincent
authors.initials: V
authors.lastname: Dubois
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Botton
authors.forename: Vérane
authors.initials: V
authors.lastname: Sarnette
authors.forename: Olivier
authors.initials: O
authors.lastname: Le Gall
authors.forename: Thierry
authors.initials: T
authors.lastname: Candresse
authors.forename: Brigitte
authors.initials: B
authors.lastname: Maisonneuve
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D049452:Green Fluorescent Proteins; D018545:Lactuca / Q000821:virology*; D008164:Luminescent Proteins / Q000032:analysis; D010935:Plant Diseases / Q000821:virology; D017800:Potyvirus / Q000254:growth & development* / Q000302:isolation & purification / Q000472:pathogenicity; D011994:Recombinant Proteins / Q000032:analysis; D014162:Transfection; D014773:Virology / Q000379:methods
chemical_list: D008164:Luminescent Proteins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins