[Expression of TGF-beta receptor II in periretinal membranes of proliferative vitreoretinopathy].
OBJECTIVE
To investigate the expression of transforming growth factor-beta receptor II (TGF-beta R II) in periretinal membranes of proliferative vitreoretinopathy (PVR).


METHODS
Immunohistochemistry and in situ hybridization were used to detect the expression of TGF-beta R II protein and mRNA in sixteen proliferative membranes of thirteen PVR patients.


RESULTS
Immunohistochemically staining showed that membranes labeled negative "-", weak positive "+", positive "2+" and strong positive staining "3+" were respectively noted one, one, three and four membranes in nine C2-C3 membranes. Total positive cell rate is 88.9%. Among seven D1-D3 membranes, one membrane was labeled "-", three were "+" and another three membranes were labeled "2+". Total positive cell rate is 85.7%. Positive staining cells were of a type of epithelial-like cells which frequently found pigment in or around them. Relation between TGF-beta R II expression and membrane grades appeared no correlation (P > 0.05). Results of in situ hybridization were almost consistent with that of immunohistochemistry.


CONCLUSIONS
Both TGF-beta R II protein and mRNA are highly expressed and up-regulated in PVR membranes by cytokine stimulation, moreover TGF-beta may play important role in the pathogenesis of PVR.
Guo
Changmei
C
Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Hui
Yannian
Y
Ma
Jixian
J
Han
Quanhong
Q
Yan
Feng
F
Qin
Xiangyang
X
Wang
Jianzhou
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010857:Pigment Epithelium of Eye / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics; D012163:Retinal Detachment / Q000150:complications / Q000378:metabolism; D018630:Vitreoretinopathy, Proliferative / Q000150:complications / Q000378:metabolism*
D012333:RNA, Messenger; D018125:Receptors, Transforming Growth Factor beta; D017346:Protein Serine-Threonine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II