Sodium dodecyl sulfate-capillary gel electrophoresis of polyethylene glycolylated interferon alpha.
Sodium dodecyl sulfate-capillary gel electrophoresis (SDS-CGE) using a hydrophilic replaceable polymer network matrix was applied to characterize the polyethylene glycol(PEG)ylated interferon alpha (PEG-IFN). The SDS-CGE method resulted in a clearer resolution in both the PEG-IFN species and the native IFN species. The distribution profile of PEGylation determined by SDS-CGE was consistent with that obtained by SDS-polyacrylamide gel electrophoresis (PAGE) with Coomassie blue or barium iodide staining. The result was also compared using matrix-assisted laser desorption/ionization-time of flight-mass spectrometry. SDS-CGE was also useful for monitoring the PEGylation reaction to optimize the reaction conditions, such as reaction molar ratio. This study shows the potential of SDS-CGE as a new method for characterizing the PEGylated proteins with advantages of speed, minimal sample consumption and high resolution.
Na
Dong H
DH
Drug Targeting Laboratory, College of Pharmacy, SungKyunKwan University, Suwon City, Korea.
Park
Eun J
EJ
Youn
Yu S
YS
Moon
Byung W
BW
Jo
Yeong W
YW
Lee
Sung H
SH
Kim
Won-Bae
WB
Sohn
Yeowon
Y
Lee
Kang C
KC
D019075:Electrophoresis, Capillary / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D016898:Interferon-alpha / Q000032:analysis*; D011092:Polyethylene Glycols / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D016898:Interferon-alpha; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols