title: Comparison of repair techniques in rat duodonal perforations: simple closure, simple closure and omentoplasty, and fibrin tissue adhesive.
abstract: BACKGROUND
  We compared three repair techniques, namely, simple closure and omentoplasty, simple closure alone, and fibrin tissue adhesive, in the treatment of rat duodenal perforations induced in the postpyloric region.
  
  
  METHODS
  Thirty male Sprague-Dawley rats (210-240 g) were included. Under ketamine and ether anesthesia, duodenal perforations of 2 mm were induced in all rats in the postpyloric region. The rats were assigned to three groups equal in number, which underwent repair with the use of simple closure and omentoplasty, simple closure alone, and fibrin glue, respectively. All the animals were sacrificed under deep ether anesthesia on the fourth postoperative day. To assess wound strength, bursting pressure measurements were performed. In addition, hydroxyproline content of the wound site was measured by absorbance spectrophotometry at 560 nm.
  
  
  RESULTS
  The mean bursting pressure in the simple closure and omentoplasty group was significantly higher than those of the simple closure (p<0.05) and fibrin glue (p<0.001) groups. No significant difference existed between the simple closure and fibrin glue groups in this respect (p>0.05). Hydroxyproline values of the simple closure and omentoplasty (p<0.05) and simple closure (p<0.001) groups were significantly lower than that of the fibrin glue group.
  
  
  CONCLUSIONS
  Fibrin tissue adhesives may have an application in the treatment of duodenal ulcer perforations, as an adjunct to laparoscopic or open surgical methods.
authors.affiliation: Department of General Surgery, Sişli Etfal Research and Training Hospital, Istanbul, Turkey. cemal_kaya@mynet.com
authors.forename: Cemal
authors.initials: C
authors.lastname: Kaya
authors.forename: Uygar
authors.initials: U
authors.lastname: Demir
authors.forename: Halil
authors.initials: H
authors.lastname: Coşkun
authors.forename: Ali
authors.initials: A
authors.lastname: Kalyoncu
authors.forename: Burhan
authors.initials: B
authors.lastname: Gündüz
authors.forename: Tülay
authors.initials: T
authors.lastname: Eroğlu
authors.forename: Birol
authors.initials: B
authors.lastname: Ağca
authors.forename: Mehmet
authors.initials: M
authors.lastname: Mihmanli
mesh_terms: D000818:Animals; D013505:Digestive System Surgical Procedures / Q000379:methods*; D008297:Male; D009852:Omentum / Q000601:surgery; D010439:Peptic Ulcer Perforation / Q000601:surgery*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014014:Tissue Adhesives; D014945:Wound Healing
chemical_list: D014014:Tissue Adhesives