Two-dimensional reference map of Candida albicans hyphal forms.
We have set up an in vitro model of culture of Candida albicans fungal cells that mimics the physiological conditions found in human beings in order to carry out studies of host-pathogen interaction. Under these conditions, C. albicans undergoes dimorphic transition (yeast-hyphae); this morphological change has been proposed as a virulence factor for this fungus. C. albicans cytoplasmic extract from hyphae cells was analyzed by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and a reference map was obtained. Protein identification was carried out by peptide mass fingerprinting or sequence tagging using a matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) or a MALDI-TOF/TOF mass spectrometer. A total of 106 spots, excised from 2-D gels, were analyzed. This resulted in the identification of 43 proteins involved in metabolism, 13 involved in transcription, protein synthesis, and fate, 8 involved in cell rescue, virulence, and defense, and 2 proteins of unknown function. This reference map is an important tool for future studies of protein differential expression after host-pathogen interaction.
Hernández
Rosa
R
Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Nombela
Cesar
C
Diez-Orejas
Rosalía
R
Gil
Concha
C
D002176:Candida albicans / Q000166:cytology / Q000378:metabolism* / Q000472:pathogenicity; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D025301:Hyphae / Q000166:cytology / Q000378:metabolism* / Q000472:pathogenicity; D010449:Peptide Mapping; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D020543:Proteome