p53 Protein interacts specifically with the meiosis-specific mammalian RecA-like protein DMC1 in meiosis.
The tumor suppressor protein p53 is specifically expressed during meiosis in spermatocytes. Subsets of p53 knockout mice exhibit testicular giant cell degenerative syndrome, which suggests p53 may be associated with meiotic cell cycle and/or DNA metabolism. Here, we show that p53 binds to the mouse meiosis-specific RecA-like protein Mus musculus DMC1 (MmDMC1). The C-terminal domain (amino acid 234-340) of MmDMC1 binds to DNA-binding domain of p53 protein. p53 might be involved in homologous recombination and/or checkpoint function by directly binding to DMC1 protein to repress genomic instability in meiotic germ cells.
Habu
Toshiyuki
T
Department of Molecular Embryology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565, Japan.
Wakabayashi
Nobunao
N
Yoshida
Kayo
K
Yomogida
Kenntaro
K
Nishimune
Yoshitake
Y
Morita
Takashi
T
D000251:Adenosine Triphosphatases / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000378:metabolism* / Q000502:physiology; D002460:Cell Line; D017931:DNA Primers; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D008540:Meiosis / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009687:Nuclear Proteins; D028044:Phosphate-Binding Proteins; D011485:Protein Binding; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism* / Q000502:physiology
D018797:Cell Cycle Proteins; D017931:DNA Primers; D004268:DNA-Binding Proteins; C513032:Dmc1 protein, mouse; D009687:Nuclear Proteins; D028044:Phosphate-Binding Proteins; D016159:Tumor Suppressor Protein p53; D000251:Adenosine Triphosphatases; C074556:DMC1 protein, human