title: HPLC electrospray mass spectrometric characterization of trimeric building blocks for oligonucleotide synthesis.
abstract: Trimeric nucleotide building blocks are valuable in synthesis of randomized oligonucleotides. In this study, we have developed HPLC-MS and HPLC-MS/MS methods for quality control of protected trinucleotides. C18 reversed-phase HPLC was used for purity evaluation, and base sequences were verified using negative ion electrospray ionization mass spectrometry (ESI-MS) and collision induced dissociation (CID) MS/MS. The principal dissociation pathway was formation of w-ions, which represent the 3'-5' direction. The other major fragments were d-ions, which are formed by cleavage of 5' C-O bonds of the sugars. The developed method was suitable for verification of purity and structure of the 29 trinucleotides studied.
authors.affiliation: Department of Pharmaceutical Chemistry, University of Kuopio, Fin-70211 Kuopio, Finland. mauriala@hytti.uku.fi
authors.forename: Timo
authors.initials: T
authors.lastname: Mauriala
authors.forename: Seppo
authors.initials: S
authors.lastname: Auriola
authors.forename: Alex
authors.initials: A
authors.lastname: Azhayev
authors.forename: Alexei
authors.initials: A
authors.lastname: Kayushin
authors.forename: Maria
authors.initials: M
authors.lastname: Korosteleva
authors.forename: Anatoly
authors.initials: A
authors.lastname: Miroshnikov
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D009841:Oligonucleotides / Q000032:analysis* / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D009841:Oligonucleotides