Antiproliferative activity of the main constituents from Phyllanthus emblica.
Eighteen main compounds, including four norsesquiterpenoids (1-4) and 14 phenolic compounds (5-18) isolated previously from Phyllanthus emblica, together with a main constituent, proanthocyanidin polymers (19) identified at this time from the roots, were estimated for their antiproliferative activities against MK-1 (human gastric adenocarcinoma), HeLa (human uterine carcinoma), and B16F10 (murine melanoma) cells using an MTT method. All of the phenolic compounds including the major components 5-8 from the fruit juice, 8, 9, and 12 from the branches and leaves, and 19 from the roots showed stronger inhibition against B16F10 cell growth than against HeLa and MK-1 cell growth. Norsesquiterpenoid glycosides 3 and 4 from the roots exhibited significant antiproliferative activities, although their aglycon 1 and monoglucoside 2 showed no inhibitory activity against these tumor cells.
Zhang
Ying-Jun
YJ
Faculty of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo Machi, Nagasaki 852-8521, Japan. zhangyj@mail.kib.ac.cn
Nagao
Tsuneatsu
T
Tanaka
Takashi
T
Yang
Chong-Ren
CR
Okabe
Hikaru
H
Kouno
Isao
I
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D005638:Fruit / Q000737:chemistry; D006131:Growth Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology; D029842:Phyllanthus emblica / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000494:pharmacology
D000972:Antineoplastic Agents, Phytogenic; D006131:Growth Inhibitors; D010636:Phenols; D012717:Sesquiterpenes