[The study of hBMP-4 gene modified tissue-engineered bone].
OBJECTIVE
Bone marrow stromal cells (bMSCs) of rabbits transferred with mammalian hBMP-4 expression plasmid were used to construct tissue-engineered bone. Gene therapy combined with tissue-engineering technique was explored to further improve osteogenesis.


METHODS
pEGFP-hBMP-4 plasmid was constructed by subcloning technique. bMSCs were then transferred with either pEGFP-hBMP-4, pEGFP plasmid by lipofectamine or left uninfected in vitro. The cells from the 3 groups were combined with natural non-organic bone (NNB) to construct tissue-engineered bones, which were subcutaneously implanted into nude mice (6 implants per group) for 4 weeks. Specimens were evaluated through histological and computerized new bone formation analysis.


RESULTS
pEGFP-hBMP-4 plasmid was successfully constructed. bMSCs could attach and proliferate on the surface on NNB. In vivo experiment showed that new bone formation in pEGFP-hBMP-4 group was higher than those of the control groups.


CONCLUSIONS
Tissue-engineered bone using hBMP-4 gene modified bMSCs might be an ideal alternative for the repair of bone.
Jiang
Xin-quan
XQ
Department of Oral and Maxillofacial Surgery, School of Stomatology, Shanghai Second Medical University, China.
Zhang
Zhi-yuan
ZY
Chen
Jian-guo
JG
Ma
Gui-xiang
GX
Chen
Chuan-jun
CJ
D000818:Animals; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins / Q000235:genetics*; D015316:Genetic Therapy; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D010012:Osteogenesis; D011817:Rabbits; D023822:Tissue Engineering
C525809:BMP4 protein, human; C525810:Bmp4 protein, mouse; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins