Blue light differentially modulates cell survival and growth.
Previous studies have reported that blue light (400-500 nm) inhibits cell mitochondrial activity. We investigated the hypothesis that cells with high energy consumption are most susceptible to blue-light-induced mitochondrial inhibition. We estimated cell energy consumption by population doubling time, and cell survival and growth by succinate dehydrogenase (SDH) activity. Six cell types were exposed to 5 or 60 J/cm(2) of blue light from quartz-tungsten-halogen (QTH), plasma-arc (PAC), or argon laser sources in monolayer culture. Post-light SDH activity correlated positively with population doubling time (R(2) = 0.91 for PAC, 0.76 for QTH, 0.68 for laser); SDH activity increased for cell types with the longest doubling times and was suppressed for cell types with shorter doubling times. Thus, light-induced exposure differentially affects SDH activity, cell survival, and growth, depending on cell energy consumption. Blue light may be useful as a therapeutic modulator of cell growth and survival.
Wataha
J C
JC
Department of Oral Rehabilitation, Medical College of Georgia School of Dentistry, Augusta, GA 30912-1260, USA. watahaj@mail.mcg.edu
Lewis
J B
JB
Lockwood
P E
PE
Hsu
S
S
Messer
R L
RL
Rueggeberg
F A
FA
Bouillaguet
S
S
D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology; D002277:Carcinoma / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002455:Cell Division / Q000528:radiation effects*; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000528:radiation effects*; D005260:Female; D005347:Fibroblasts / Q000201:enzymology / Q000528:radiation effects; D005881:Gingiva / Q000473:pathology; D006801:Humans; D015603:Keratinocytes / Q000201:enzymology / Q000528:radiation effects; D007834:Lasers; D008027:Light; D008029:Lighting / Q000295:instrumentation; D008168:Lung / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008928:Mitochondria / Q000528:radiation effects; D009062:Mouth Neoplasms / Q000473:pathology; D012867:Skin / Q000473:pathology; D013385:Succinate Dehydrogenase / Q000528:radiation effects
D013385:Succinate Dehydrogenase