title: T cell activation by lipopeptide antigens.
abstract: Unlike major histocompatibility proteins, which bind peptides, CD1 proteins display lipid antigens to T cells. Here, we report that CD1a presents a family of previously unknown lipopeptides from Mycobacterium tuberculosis, named didehydroxymycobactins because of their structural relation to mycobactin siderophores. T cell activation was mediated by the alphabeta T cell receptors and was specific for structure of the acyl and peptidic components of these antigens. These studies identify a means of intracellular pathogen detection and identify lipopeptides as a biochemical class of antigens for T cells, which, like conventional peptides, have a potential for marked structural diversity.
authors.affiliation: Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital and Harvard Medical School, Smith Building Room 514, 1 Jimmy Fund Way, Boston, MA 02115, USA. bmoody@rics.bwh.harvard.edu
authors.forename: D Branch
authors.initials: DB
authors.lastname: Moody
authors.forename: David C
authors.initials: DC
authors.lastname: Young
authors.forename: Tan-Yun
authors.initials: TY
authors.lastname: Cheng
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Rosat
authors.forename: Carme
authors.initials: C
authors.lastname: Roura-Mir
authors.forename: Peter B
authors.initials: PB
authors.lastname: O'Connor
authors.forename: Dirk M
authors.initials: DM
authors.lastname: Zajonc
authors.forename: Andrew
authors.initials: A
authors.lastname: Walz
authors.forename: Marvin J
authors.initials: MJ
authors.lastname: Miller
authors.forename: Steven B
authors.initials: SB
authors.lastname: Levery
authors.forename: Ian A
authors.initials: IA
authors.lastname: Wilson
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Costello
authors.forename: Michael B
authors.initials: MB
authors.lastname: Brenner
mesh_terms: D017951:Antigen Presentation; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D018949:Antigens, CD1 / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D006900:Hydroxylation; D008074:Lipoproteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation; D008958:Models, Molecular; D009169:Mycobacterium tuberculosis / Q000254:growth & development / Q000276:immunology*; D010080:Oxazoles / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D011487:Protein Conformation; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection
chemical_list: D000942:Antigens, Bacterial; D018949:Antigens, CD1; C419393:CD1a antigen; D008074:Lipoproteins; D010080:Oxazoles; D016693:Receptors, Antigen, T-Cell, alpha-beta; C018608:mycobactins