Iron chelation can modulate UVA-induced lipid peroxidation and ferritin expression in human reconstructed epidermis.
OBJECTIVE
As ferritin has been identified as an important factor in antioxidant defense in cultured human skin cells, we evaluated UVA-induced lipid hydroperoxides (LPO) production and ferritin expression in reconstructed human epidermis in vitro.


RESULTS
Ferritin is regularly present in the basal layer of unirradiated epidermis both in the human skin in vivo and in the reconstructed human epidermis in vitro. Following acute UVA exposure, ferritin expression increased in basal epidermal cells in both models. Quantitative analysis showed that, in reconstructed human epidermis, LPO and ferritin levels increased linearly with the UVA dose. An iron chelator, OR10141, inhibited these inductions.


CONCLUSIONS
These findings demonstrate that reconstructed human epidermis is a useful in vitro model to study UVA-induced oxidative stress and protection afforded by iron chelators, antioxidants or UVA absorbers.
Seité
Sophie
S
L'Oréal Recherche, Clichy, France. sseite@recherche.loreal.com
Popovic
Evelyne
E
Verdier
Marie Paule
MP
Roguet
Roland
R
Portes
Pascal
P
Cohen
Catherine
C
Fourtanier
Anny
A
Galey
Jean Baptiste
JB
D000328:Adult; D046508:Culture Techniques; D004492:Edetic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005293:Ferritins / Q000378:metabolism*; D006801:Humans; D007502:Iron Chelating Agents / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects*; D008297:Male; D018384:Oxidative Stress / Q000528:radiation effects; D011829:Radiation Dosage; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays
D007502:Iron Chelating Agents; C097359:N,N'-bis(3,4,5-trimethoxybenzyl)ethylenediamine N,N'-diacetic acid; D005293:Ferritins; D004492:Edetic Acid