[The expression of cytochrome P450 1A1 of endothelial cells induced by benzo(a)pyrene].
OBJECTIVE
To explore the effect of benzo(a)pyrene (BaP) on the expression and the activities of cytochrome P450 1A1 (CYP1A1) of porcine aortic endothelial cells.


METHODS
Porcine aortic endothelial cells were cultured in vitro, and treated with different concentrations of BaP (0, 0.5, 1.0, 5.0, 10.0 micro mol/L) for 24 hours, CYP1A1 expression was determined by Western blot and immunohistochemistry. At the same time, the ethoxyresorufin-o-deethylase (EROD) activities were measured by spectrofluorometer.


RESULTS
By Western blot, the expression of CYP1A1 of control cells was not found, but the expression of CYP1A1 of cells treated with BaP was found; By immunohistochemistry, only part of endothelial cells treated with BaP had positive expression of CYP1A1. The peak activities of EROD induced by BaP was at the concentration of 0.5 - 1.0 micro mol/L.


CONCLUSIONS
BaP could induce part of endothelial cells to synthesize CYP1A1. BaP of 0.5 - 1.0 micro mol/L could induce peak activities of EROD.
Yang
Jin-bo
JB
Department of Labor Health and Environment Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Jiang
Chang-zheng
CZ
Ke
Lei
L
Xu
Qian
Q
Yang
Miao
M
He
Han-zhen
HZ
Hu
Bing-chang
BC
Wu
Tang-chun
TC
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D015153:Blotting, Western; D002478:Cells, Cultured; D019363:Cytochrome P-450 CYP1A1 / Q000032:analysis*; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D007150:Immunohistochemistry; D013552:Swine
D001564:Benzo(a)pyrene; D019363:Cytochrome P-450 CYP1A1