[Cationic trypsinogen gene mutation in patients with chronic idiopathic pancreatitis].
OBJECTIVE
Mutation of Cationic trypsinogen gene is clearly associated with hereditary pancreatitis and plays an important role in the pathogenesis of pancreatitis. According to literature, this mutation is occasionally occurred in patients with pancreatitis in Western countries and Japan. The aim of this study was to find out whether the mutation was observed in Korean patients with chronic idiopathic pancreatitis.


METHODS
Peripheral blood samples of 11 patients with chronic idiopathic pancreatitis were collected consecutively, and DNA was extracted from the samples. Polymerase chain reaction was performed in exon 2 and 3 of cationic trypsinogen gene. Then, DNA products were purified and sequenced.


RESULTS
The mutation was not found in exon 2 and 3 of cationic trypsinogen gene in these patients.


CONCLUSIONS
There was no cationic trypsinogen mutation in Korean patients with chronic idiopathic pancreatitis. Further large sampled cohort study is needed.
Lee
Woo Jin
WJ
Center for Liver Cancer, National Cancer Center, Goyang, Korea.
Kim
Kyung-Ah
KA
Lee
June Sung
JS
Jeon
Young Bin
YB
Jeong
Ji Bong
JB
Ryu
Ji Kon
JK
Kim
Yong-Tae
YT
Yoon
Yong Bum
YB
Kim
Chung Yong
CY
D000293:Adolescent; D000328:Adult; D002908:Chronic Disease; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010195:Pancreatitis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014357:Trypsin / Q000235:genetics*; D014362:Trypsinogen / Q000235:genetics*
D014362:Trypsinogen; C489303:PRSS1 protein, human; D014357:Trypsin