A transgene encoding a blue-light receptor, phot1, restores blue-light responses in the Arabidopsis phot1 phot2 double mutant.
Phototropins (phot1 and phot2) are suggested to be multifunctional blue-light (BL) receptors mediating phototropism, chloroplast movement, stomatal opening, and leaf expansion. The Arabidpsis phot1 phot2 double mutant lacks all of these responses. To confirm the requirement of phototropins in BL responses, the Arabidopsis phot1 phot2 double mutant was transformed with PHOT1 cDNA and the phenotypic restoration was analysed in the transformants. It was found that all BL responses were restored, although differentially, by the transformation of the Arabidopsis phot1 phot2 double mutant with PHOT1 cDNA. The results showed that phot1 was an essential component for all these BL responses in planta, and that the cellular level of phot1 might determine the individual BL responses.
Doi
Michio
M
Research and Development Center for Higher Education, Kyushu University, Ropponmatsu, Fukuoka 810-8560, Japan.
Shigenaga
Ayako
A
Emi
Takashi
T
Kinoshita
Toshinori
T
Shimazaki
Ken-Ichiro
K
D017360:Arabidopsis / Q000235:genetics* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D007700:Kinetics; D008027:Light; D010641:Phenotype; D010750:Phosphoproteins / Q000235:genetics*; D018515:Plant Leaves / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases; D014170:Transformation, Genetic
D029681:Arabidopsis Proteins; D017931:DNA Primers; D018076:DNA, Complementary; C423903:PHOT2 protein, Arabidopsis; D010750:Phosphoproteins; C103646:NPH1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases