[Study on ribotyping of Lyme borreliosis spirochete in Guizhou province].
OBJECTIVE
To define the main genotypes in Guizhou agricultural areas by molecular epidemiologic investigation of 21 Borrelia burgdorferi sensu lato of Lyme disease spirochetes and to provide the scientific bases for formulating a preventive policy.


METHODS
Polymerase chain reaction (PCR) technique was used to amplify the 23S(rrl)-5S(rrf) intergenic spacer, and amplified products were analyzed by restriction fragment length polymorphism (RFLP) and nucleotide sequencing.


RESULTS
There were two genospecies in the strains: 20 strains belong to Borrelia valaisiana, 1 strain is Borelia sp.


CONCLUSIONS
Borrelia valaisiana was the main genotype in Guizhou agricultural areas. The harmness of B. valaisiana to human being has been confirmed. In order to efficiently prevent the harmness of agent to the people in Guizhou agriculture areas, we should study the risk further.
Wang
Ding-ming
DM
Guizhou Center for Disease Control and Prevention, Guiyang 550004, China.
Hao
Qin
Q
Cai
Xing-he
XH
Wan
Kang-lin
KL
Wang
Zhao-xiao
ZX
Chen
Jian
J
D001483:Base Sequence; D025065:Borrelia burgdorferi / Q000145:classification / Q000235:genetics*; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D006801:Humans; D008193:Lyme Disease / Q000382:microbiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D021521:Ribotyping; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D012338:RNA, Ribosomal, 23S; D012341:RNA, Ribosomal, 5S