Experimental diabetes accelerates accumulation of fluorescent pigments in rat trigeminal neurons.
The purpose of this study was to investigate autofluorescent pigment granules (lipofuscin, ceroid) in the trigeminal neurons (TN) during aging and streptozotocin-induced diabetes. Four young adult male rats were injected with streptozotocin (STZ; 50 mg/kg) to produce diabetes (DM), for comparison with four young uninjected control rats and four aged rats (90 weeks old). Eight weeks after STZ injection, all rats were fixed with 4% paraformaldehyde, and paraffin sections of TN were prepared and observed by fluorescence microscopy. We found the number of neurons with autofluorescent pigments had increased to 30.38% of the total in DM compared to 8.98% in the control group, and 25.36% in the aged rats. The area of autofluorescence within those neurons was 16.84% in aged rats, 13.02% in DM and 4.45% in the controls. Thus, DM caused accelerated accumulation of fluorescent granules in trigeminal neurons, and this change may show that premature aging contributes to neuronal functional decline and morphological change.
Sugaya
Akira
A
Department of Periodontology, Kanagawa Dental College, 82, Inaoka-cho, Yokosuka, 238-8580, Japan. sugayaak@kdcnet.ac.jp
Sugimioto
Hisashi
H
Mogi
Nobumichi
N
Tsujigami
Hiroshi
H
Deguchi
Shinji
S
D019588:Aging, Premature / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D000818:Animals; D002566:Ceroid / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D003929:Diabetic Neuropathies / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D004195:Disease Models, Animal; D005453:Fluorescence; D017127:Glycation End Products, Advanced / Q000378:metabolism; D008062:Lipofuscin / Q000378:metabolism*; D008297:Male; D009410:Nerve Degeneration / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009475:Neurons, Afferent / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D012668:Trigeminal Ganglion / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology
D002566:Ceroid; D017127:Glycation End Products, Advanced; D008062:Lipofuscin; D009419:Nerve Tissue Proteins