title: Antigen-specific CD8+ T cell responses in intestinal tissues during murine listeriosis.
abstract: Infection of mice with Listeria monocytogenes induces a strong CD8+ T cell response, which is critical for the control of bacteria and for protection against re-infection. We analyzed the CD8+ T cell response in different intestinal tissues following oral and intravenous (i.v.) L. monocytogenes infection. After oral infection, bacterial titers in small intestine and large intestine, and the listeria-specific CD8+ T cell response in the mucosa of both parts of the intestine, were highly correlated. Oral infection of CD28-deficient mice revealed that this response was strictly dependent on CD28 costimulation. Significant listeria-specific CD8+ T cell responses also occurred in all intestinal tissues analyzed after i.v. infection or after DNA vaccination, indicating that the accumulation of listeria-specific CD8+ T cells in these tissues only partially depends on local antigen presentation and inflammation.
authors.affiliation: Max-Planck Institute for Infection Biology, Schumannstr. 21/22, 10117, Berlin, Germany.
authors.forename: Mischo
authors.initials: M
authors.lastname: Kursar
authors.forename: Kerstin
authors.initials: K
authors.lastname: Bonhagen
authors.forename: Anne
authors.initials: A
authors.lastname: Köhler
authors.forename: Thomas
authors.initials: T
authors.lastname: Kamradt
authors.forename: Stefan H E
authors.initials: SH
authors.lastname: Kaufmann
authors.forename: Hans Willi
authors.initials: HW
authors.lastname: Mittrücker
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage; D018106:CD28 Antigens / Q000235:genetics / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D004195:Disease Models, Animal; D007413:Intestinal Mucosa / Q000276:immunology*; D008089:Listeria monocytogenes; D008088:Listeriosis / Q000276:immunology* / Q000517:prevention & control; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage
chemical_list: D001428:Bacterial Vaccines; D018106:CD28 Antigens; D019444:Vaccines, DNA