title: A microsatellite map of the African human malaria vector Anopheles funestus.
abstract: Microsatellite markers and chromosomal inversion polymorphisms are useful genetic markers for determining population structure in Anopheline mosquitoes. In Anopheles funestus (2N = 6), only chromosome arms 2R, 3R, and 3L are known to carry polymorphic inversions. The physical location of microsatellite markers with respect to polymorphic inversions is potentially important information for interpreting population genetic structure, yet none of the available marker sets have been physically mapped in this species. Accordingly, we mapped 32 polymorphic A. funestus microsatellite markers to the polytene chromosomes using fluorescent in situ hybridization (FISH) and identified 16 markers outside of known polymorphic inversions. Here we provide an integrated polytene chromosome map for A. funestus that includes the breakpoints of all known polymorphic inversions as well as the physical locations of microsatellite loci developed to date. Based on this map, we suggest a standard set of 16 polymorphic microsatellite markers that are distributed evenly across the chromosome complement, occur predominantly outside of inversions, and amplify reliably. Adoption of this set by researchers working in different regions of Africa will facilitate metapopulation analyses of this primary malaria vector.
authors.affiliation: Center for Tropical Disease Research and Training, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA.
authors.forename: I
authors.initials: I
authors.lastname: Sharakhov
authors.forename: O
authors.initials: O
authors.lastname: Braginets
authors.forename: O
authors.initials: O
authors.lastname: Grushko
authors.forename: A
authors.initials: A
authors.lastname: Cohuet
authors.forename: W M
authors.initials: WM
authors.lastname: Guelbeogo
authors.forename: D
authors.initials: D
authors.lastname: Boccolini
authors.forename: M
authors.initials: M
authors.lastname: Weill
authors.forename: C
authors.initials: C
authors.lastname: Costantini
authors.forename: N'F
authors.initials: N
authors.lastname: Sagnon
authors.forename: D
authors.initials: D
authors.lastname: Fontenille
authors.forename: G
authors.initials: G
authors.lastname: Yan
authors.forename: N J
authors.initials: NJ
authors.lastname: Besansky
mesh_terms: D000349:Africa; D000818:Animals; D000852:Anopheles / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D004199:Disease Vectors; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008288:Malaria / Q000469:parasitology; D018895:Microsatellite Repeats / Q000235:genetics*