title: The genome sequence of Mycoplasma mycoides subsp. mycoides SC type strain PG1T, the causative agent of contagious bovine pleuropneumonia (CBPP).
abstract: Mycoplasma mycoides subsp. mycoidesSC (MmymySC)is the etiological agent of contagious bovine pleuropneumonia (CBPP), a highly contagious respiratory disease in cattle. The genome of Mmymy SC type strain PG1(T) has been sequenced to map all the genes and to facilitate further studies regarding the cell function of the organism and CBPP. The genome is characterized by a single circular chromosome of 1211703 bp with the lowest G+C content (24 mole%)and the highest density of insertion sequences (13% of the genome size)of all sequenced bacterial genomes. The genome contains 985 putative genes, of which 72 are part of insertion sequences and encode transposases. Anomalies in the GC-skew pattern and the presence of large repetitive sequences indicate a high genomic plasticity. A variety of potential virulence factors was identified, including genes encoding putative variable surface proteins and enzymes and transport proteins responsible for the production of hydrogen peroxide and the capsule, which is believed to have toxic effects on the animal.
authors.affiliation: Department of Biotechnology, Royal Institute of Technology (KTH), SE-106 91 Stockholm, Sweden.
authors.forename: Joakim
authors.initials: J
authors.lastname: Westberg
authors.forename: Anja
authors.initials: A
authors.lastname: Persson
authors.forename: Anders
authors.initials: A
authors.lastname: Holmberg
authors.forename: Alexander
authors.initials: A
authors.lastname: Goesmann
authors.forename: Joakim
authors.initials: J
authors.lastname: Lundeberg
authors.forename: Karl-Erik
authors.initials: KE
authors.lastname: Johansson
authors.forename: Bertil
authors.initials: B
authors.lastname: Pettersson
authors.forename: Mathias
authors.initials: M
authors.lastname: Uhlén
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics; D001483:Base Sequence / Q000235:genetics; D001692:Biological Transport / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D004261:DNA Replication / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D005815:Genetic Code / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D016680:Genome, Bacterial; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009175:Mycoplasma Infections / Q000150:complications; D009176:Mycoplasma mycoides / Q000235:genetics*; D011002:Pleuropneumonia, Contagious / Q000382:microbiology*; D014176:Protein Biosynthesis / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D013045:Species Specificity; D014158:Transcription, Genetic; D037521:Virulence Factors / Q000235:genetics
chemical_list: D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D008565:Membrane Proteins; D037521:Virulence Factors