Polyphasic characterization of xanthomonads isolated from onion, garlic and Welsh onion (Allium spp.) and their relatedness to different Xanthomonas species.
Bacterial blight is an emerging disease that affects primarily onion, but also garlic and Welsh onion. The present study was undertaken to characterize the causative xanthomonad(s) by a polyphasic approach using a worldwide collection of 33 bacterial strains. Analysis of 16S rRNA gene sequence similarities indicated that the causal agent belongs to the campestris core in the genus Xanthomonas, which is in agreement with results of phenotypic characterization (analyses of carbon source utilization and fatty acid methyl esters). However, DNA-DNA hybridization, thermal stability of DNA reassociation and fluorescent amplified fragment length polymorphism analysis allowed the causal agent to be identified as a pathovar of Xanthomonas axonopodis.
Roumagnac
P
P
CIRAD, UMR Peuplement végétaux et Bioagresseurs en Milieu Tropical CIRAD/Université de la Réunion, Ligne Paradis, 97410 Saint Pierre, Réunion Island, France.
Gagnevin
L
L
CIRAD, UMR Peuplement végétaux et Bioagresseurs en Milieu Tropical CIRAD/Université de la Réunion, Ligne Paradis, 97410 Saint Pierre, Réunion Island, France.
Gardan
L
L
UMR de Pathologie Végétale, INRA, Station de Pathologie Végétale, 42 rue Georges Morel, BP 57, 49071 Beaucouzé Cedex, France.
Sutra
L
L
UMR de Pathologie Végétale, INRA, Station de Pathologie Végétale, 42 rue Georges Morel, BP 57, 49071 Beaucouzé Cedex, France.
Manceau
C
C
UMR de Pathologie Végétale, INRA, Station de Pathologie Végétale, 42 rue Georges Morel, BP 57, 49071 Beaucouzé Cedex, France.
Dickstein
E R
ER
Department of Plant Pathology, University of Florida, PO Box 110680, Gainesville, FL 32611-0680, USA.
Jones
J B
JB
Department of Plant Pathology, University of Florida, PO Box 110680, Gainesville, FL 32611-0680, USA.
Rott
P
P
UMR 385 ENSAM-INRA-CIRAD, Biologie et Génétique des Interactions Plante-Parasite, Avenue Agropolis, 34398 Montpellier Cedex 5, France.
Pruvost
O
O
CIRAD, UMR Peuplement végétaux et Bioagresseurs en Milieu Tropical CIRAD/Université de la Réunion, Ligne Paradis, 97410 Saint Pierre, Réunion Island, France.
D000490:Allium / Q000382:microbiology*; D017931:DNA Primers; D005737:Garlic / Q000382:microbiology*; D008969:Molecular Sequence Data; D019697:Onions / Q000382:microbiology*; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014974:Xanthomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity
D017931:DNA Primers; D012336:RNA, Ribosomal, 16S