title: Sodium chloride fixation of tissues under field conditions in tropical countries.
abstract: Fixation of skin and lymph node fragments in anhydric sodium chloride at room temperature for periods of weeks or months was found to preserve morphological structures and immunoreactivity. Fragments of a size of 6-8x6x6 mm were dried and placed in anhydric sodium chloride powder in sealed containers. Prior to further processing, the specimens were desalinated and rehydrated, then snap-frozen, sectioned, fixed in acetone and stained with monoclonal antibodies. The intensity of staining of molecular structures did not differ from that of primarily snap-frozen specimens. The method allows fixation of tissues under field conditions in tropical countries and at places lacking freezing facilities.
authors.affiliation: Department of Surgical Research and Transplantation, Medical Research Center, Polish Academy of Sciences, 5 Pawinskiego Str., 02106 Warsaw, Poland. wlo@cmdik.pan.pl
authors.forename: Waldemar L
authors.initials: WL
authors.lastname: Olszewski
authors.forename: Dorota
authors.initials: D
authors.lastname: Zolich
authors.forename: Gurusami
authors.initials: G
authors.lastname: Manokaran
authors.forename: Mani F
authors.initials: MF
authors.lastname: Tripathi
mesh_terms: D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000737:chemistry / Q000166:cytology*; D012867:Skin / Q000737:chemistry / Q000166:cytology*; D012965:Sodium Chloride / Q000737:chemistry*; D016707:Tissue Fixation / Q000379:methods*; D014329:Tropical Climate
chemical_list: D012965:Sodium Chloride