Overexpression of DRG2 suppresses the growth of Jurkat T cells but does not induce apoptosis.
Developmentally regulated GTP-binding protein (DRG) is a new subfamily within the superfamily of GTP-binding proteins. Its expression is regulated during embryonic development. To investigate the effect of the expression of DRG2 on cell growth, we constructed a human Jurkat-T-cell line that overexpresses DRG2. Overexpression of DRG2 suppressed the growth and the aggregation of Jurkat cells but did not induce apoptotic cell death. We used cDNA microarray analysis to examine the global changes in gene expression induced by an overexpression of DRG2. DNA array analyses identified genes that may suppress cell growth at a number of levels in multiple signaling cascades in Jurkat cells and also several prosurvival genes that may protect cells from apoptosis.
Ko
Myoung Seok
MS
Department of Biological Sciences and Immunomodulation Research Center, University of Ulsan, Ulsan 680-749, Republic of Korea.
Lee
Unn Hwa
UH
Kim
Sin Il
SI
Kim
Hyo Jeong
HJ
Park
Jeong Jae
JJ
Cha
Seung Ju
SJ
Kim
Sung Bum
SB
Song
Hebok
H
Chung
Dae Kyun
DK
Han
In Seob
IS
Kwack
KyuBum
K
Park
Jeong Woo
JW
D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology*; D002449:Cell Aggregation; D002452:Cell Count; D002455:Cell Division / Q000502:physiology; D005434:Flow Cytometry; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D019169:Jurkat Cells; D020411:Oligonucleotide Array Sequence Analysis; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism / Q000502:physiology
D017304:Annexin A5; C409877:DRG2 protein, human; D011994:Recombinant Proteins; D019204:GTP-Binding Proteins