title: Transfer of vitrified blastocysts from one or two superovulated Large White Hyperprolific donors to Meishan recipients: reproductive parameters at Day 30 of pregnancy.
abstract: The present study was designed to determine the effect of pooling embryos from two donors on the reproductive success of transfer of vitrified/warmed porcine blastocysts. Intact blastocysts were collected from superovulated Large White Hyperprolific gilts (n = 24) on Days 5-5.5 after artificial insemination. Embryos were recovered by flushing the uterine horns, and unhatched blastocysts were selected. Vitrification and warming were performed as described by Berthelot et al. [Cryobiology 41(2000) 116]. To evaluate in vitro development, 37 vitrified/warmed blastocysts were cultured, non-vitrified embryos (n = 48) were used as controls. Embryo transfers were conducted in asynchronous (-24 h) Meishan gilts (n = 20). Twenty vitrified/warmed blastocysts were surgically transferred into one uterine horn. Ten recipients received embryos from one donor (Group 1) and the other 10 transfers were performed with mixed embryos from two donors (Group 2). Pregnancy was assessed ultrasonographically at Day 25 after estrus and recipients were slaughtered at Day 30 after transfer. In vitro survival rate of the vitrified/warmed blastocysts was lower (P < 0.01) than that from control embryos (73.0% versus 93.7%). The pregnancy rate for Group 1 (70%) was not different (P > 0.05) than that from Group 2 (90%). No significant differences were detected between Groups 1 and 2 for in vivo embryo development (number fetuses/transferred embryos in pregnant recipients) or in vivo embryo survival (number viable fetuses/transferred embryos in pregnant recipients). However, the in vivo efficiency (number viable fetuses/total transferred embryos) was higher (P < 0.05) when transfers were performed with embryos from two donors (19.5% versus 30.5%). These results indicate that pooling embryos from two donors increases the in vivo efficiency after transfer of vitrified/warmed porcine blastocysts.
authors.affiliation: Department of Animal Medicine and Surgery, Veterinary Science (Reproducción y Obstetricia), Hospital Clínico Veterinario, University of Murcia, Spain.ccuello@um.es
authors.forename: Cristina
authors.initials: C
authors.lastname: Cuello
authors.forename: Françoise
authors.initials: F
authors.lastname: Berthelot
authors.forename: Françoise
authors.initials: F
authors.lastname: Martinat-Botté
authors.forename: Philippe
authors.initials: P
authors.lastname: Guillouet
authors.forename: Vincent
authors.initials: V
authors.lastname: Furstoss
authors.forename: Christian
authors.initials: C
authors.lastname: Boisseau
authors.forename: Patrick
authors.initials: P
authors.lastname: Manceau
authors.forename: Alain
authors.initials: A
authors.lastname: Locatelli
authors.forename: Emilio A
authors.initials: EA
authors.lastname: Martínez
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D015925:Cryopreservation / Q000662:veterinary*; D004624:Embryo Transfer / Q000662:veterinary*; D005314:Embryonic and Fetal Development; D005260:Female; D006358:Hot Temperature; D007315:Insemination, Artificial / Q000662:veterinary; D011247:Pregnancy; D013480:Superovulation; D013552:Swine / Q000502:physiology*; D020858:Tissue and Organ Harvesting / Q000662:veterinary