title: A relationship between the mevalonate pathway and isoprenoid production in actinomycetes.
abstract: Most Streptomyces strains are equipped with only the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for the formation of isopentenyl diphosphate. In addition to this pathway, some Strepromyces strains have the mevalonate pathway to produce terpenoid antibiotics. We have previously shown that a gene cluster for biosynthesis of terpentecin, a diterpene antibiotic, was located in adjacent the mevalonate pathway gene cluster. In this study, a mevalonate pathway gene cluster was cloned from Actinoplanes sp. strain A40644, an isoprenoid antibiotic BE-40644 producer, to examine whether the mevalonate pathway genes and isoprenoid biosynthetic genes are clustered in genomic DNA. By sequencing flanking regions a probable BE-40644 biosynthetic gene cluster was found in the downstream region of the mevalonate pathway gene cluster. Heterologous expression of a 9-kb fragment confirmed that a set of the BE-40644 biosynthetic genes was involved in the fragment. This result suggested that the presence of the mevalonate pathway might be a good landmark to detect the production of isoprenoid compounds by actinomycetes.
authors.affiliation: Biotechnology Research Center, Toyama Prefectural University, Toyama 939-0398, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kawasaki
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Kuzuyama
authors.forename: Kazuo
authors.initials: K
authors.lastname: Furihata
authors.forename: Nobuya
authors.initials: N
authors.lastname: Itoh
authors.forename: Haruo
authors.initials: H
authors.lastname: Seto
authors.forename: Tohru
authors.initials: T
authors.lastname: Dairi
mesh_terms: D039903:Actinobacteria / Q000235:genetics / Q000378:metabolism*; D000191:Actinomycetaceae / Q000235:genetics; D016227:Benzoquinones / Q000378:metabolism; D003001:Cloning, Molecular; D053938:DNA Fragmentation / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial / Q000235:genetics; D008798:Mevalonic Acid / Q000378:metabolism*; D005810:Multigene Family / Q000502:physiology; D017422:Sequence Analysis, DNA; D013729:Terpenes / Q000378:metabolism*
chemical_list: C095251:BE 40644; D016227:Benzoquinones; D013729:Terpenes; D008798:Mevalonic Acid