title: Feasibility and limits of bone marrow mononuclear cell expansion following irradiation.
abstract: OBJECTIVE
  To define the ability of bone marrow mononuclear cells (BMMNC) to expand after irradiation and to determine the amount of apoptosis in irradiated expanded cells.
  
  
  METHODS
  Non-human primate BMMNC were irradiated in vitro at doses ranging from 0 to 4 Gy and were cultured during 1 week in the presence of interleukin 3, interleukin 6, stem cell factor, thrombopoietin and fms-like tyrosine kinase-3 ligand. The expansion yield of BMMNC, colony-forming cells and CD34(+) cells were compared with non-irradiated control cultures. Apoptosis in expanded cells was also defined by annexin V/propidium iodine staining.
  
  
  RESULTS
  Irradiation of BMMNC up to 1 Gy did not modify the ability of haematopoietic cells to expand. At higher doses, expansion of haematopoietic cells is reduced as compared with non-irradiated cultures but it remains significant. This reduction in expansion of BMMNC was related to radiation-induced apoptosis.
  
  
  CONCLUSIONS
  The results suggest that it is possible to expand haematopoietic cells after irradiation doses at least up to 2 Gy. This suggests a possible use of cell therapy for the treatment of radiation accident victims.
authors.affiliation: Laboratoire de recherche sur les thérapies de l'irradiation, Institut de Radioprotection et de Sûreté Nucléaire, Fontenay aux roses, France. jean-marc.bertho@irsn.fr
authors.forename: J M
authors.initials: JM
authors.lastname: Bertho
authors.forename: E
authors.initials: E
authors.lastname: Mathieu
authors.forename: A
authors.initials: A
authors.lastname: Lauby
authors.forename: J
authors.initials: J
authors.lastname: Frick
authors.forename: C
authors.initials: C
authors.lastname: Demarquay
authors.forename: P
authors.initials: P
authors.lastname: Gourmelon
authors.forename: N C
authors.initials: NC
authors.lastname: Gorin
authors.forename: D
authors.initials: D
authors.lastname: Thierry
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D002454:Cell Differentiation / Q000528:radiation effects; D002455:Cell Division / Q000528:radiation effects; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D005240:Feasibility Studies; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology / Q000528:radiation effects; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D008252:Macaca fascicularis; D008297:Male; D011829:Radiation Dosage; D011836:Radiation Tolerance / Q000502:physiology*