[Preliminary study on the gene expression profiles of ependymomas with cDNA array].
OBJECTIVE
To investigate the differential gene expression of ependymomas.


METHODS
Four fresh samples of ependymomas and 1 of normal brain tissue were collected during operation. The extracted total RNAs were converted as (32)P tagged cDNA probes, which were then hybridized with the Atlas Human Cancer Array, producing the array based hybridization maps following the protocol provided with the kit. A set of special software was applied to the analysis and RT-PCR was performed to test the result.


RESULTS
In comparison with the normal brain tissue, there were 31 upregulated gene and 1 downregulated gene in ependymomas, most of which were firstly found to be differentially expressed in this kind of tumor.


CONCLUSIONS
The discrepancy of gene expression profiles between ependymomas and normal brain tissues is highly put through and effectively detected with cDNA array, which provides new information for the further research on the molecular mechanisms of this lesion.
Jiang
Rong-cai
RC
Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
Pu
Pei-yu
PY
Shen
Chang-hong
CH
Jiao
Bao-hua
BH
Yu
Shi-zhu
SZ
Kang
Chun-sheng
CS
Wang
Chun-yan
CY
Wang
Guang-xiu
GX
D001921:Brain / Q000378:metabolism; D001932:Brain Neoplasms / Q000235:genetics*; D004806:Ependymoma / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction