title: Analysis of microsatellite instability and loss of heterozygosity in breast cancer with the use of a well characterized multiplex system.
abstract: Analysis of microsatellite instability (MI) and loss of heterozygosity (LOH) is recommended for screening patients with sporadic and hereditary malignancies. This study shows an application of a fluorescent hexaplex PCR system for microsatellite typing on A.L.F. DNA Sequencer (Pharmacia Biotech). This technique detects changes in microsatellites providing a time-efficient, reliable and accurate method for MI and LOH analyses. The Fragment Manager software was used for automated size calculation and quantitation of DNA fragments, enabling rapid and precise measurement of allelic ratios. We examined 70 breast cancer and 70 control DNA specimens, classified all the patterns of microsatellite alterations, and set up MI and LOH assessment criteria for the automated multiplex fluorescent method.
authors.affiliation: Department of Human Biology, The Ludwik Rydygier Medical University of Bydgoszcz, Bydgoszcz, Poland. jolanta_powierska@o2.pl
authors.forename: Jolanta
authors.initials: J
authors.lastname: Powierska-Czarny
authors.forename: Danuta
authors.initials: D
authors.lastname: Miścicka-Sliwka
authors.forename: Jakub
authors.initials: J
authors.lastname: Czarny
authors.forename: Tomasz
authors.initials: T
authors.lastname: Grzybowski
authors.forename: Marcin
authors.initials: M
authors.lastname: Wozniak
authors.forename: Gerard
authors.initials: G
authors.lastname: Drewa
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Czechowicz
authors.forename: Jan
authors.initials: J
authors.lastname: Sir
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D043171:Chromosomal Instability; D004586:Electrophoresis; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction