Conjugate for efficient delivery of short interfering RNA (siRNA) into mammalian cells.
The efficient delivery of short interfering RNAs (siRNAs) into cells provides a powerful approach to study cellular functions. SiRNAs were coupled to the membrane permeant peptides (MPPs) penetratin and transportan to improve their uptake by cells. Thiol-containing siRNAs corresponding to luciferase, or green fluorescent protein (GFP) transgenes, were synthesized and conjugated to penetratin or transportan via a disulfide bond that is labile in the reducing environment of the cytoplasm. These MPP-siRNAs efficiently reduced transient and stable expression of reporter transgenes in several mammalian cell types in a high proportion of cells, and demonstrated equivalent or better delivery characteristics than cationic liposomes with fewer manipulations.
Muratovska
Aleksandra
A
MRC-Dunn Human Nutrition Unit, Wellcome Trust/MRC building, Hills Rd, Cambridge CB2 2XY, UK.
Eccles
Michael R
MR
D000818:Animals; D016466:CHO Cells; D019556:COS Cells; D002352:Carrier Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D057846:Cell-Penetrating Peptides; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D003593:Cytoplasm / Q000378:metabolism; D004220:Disulfides / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D019004:Galanin; D020868:Gene Silencing; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D008156:Luciferases / Q000378:metabolism; D008164:Luminescent Proteins / Q000378:metabolism; D051379:Mice; D010446:Peptide Fragments / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D019076:Transgenes; D014862:Wasp Venoms
D002352:Carrier Proteins; D057846:Cell-Penetrating Peptides; D004220:Disulfides; D008164:Luminescent Proteins; D010446:Peptide Fragments; D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins; D014862:Wasp Venoms; C110068:transportan; D049452:Green Fluorescent Proteins; D019004:Galanin; C414409:penetratin; D008156:Luciferases