title: Lack of association between human G-protein beta3 subunit variant and overweight in Japanese workers.
abstract: We examined the association of the G-protein beta3 subunit gene (GNB3) C825T polymorphism with overweight in Japanese workers. This cross-sectional study used multivariate analysis to investigate whether a polymorphism in the C825T polymorphism was associated independently with overweight when factors such as age and lifestyle were taken into account. The study in 1453 men and 1172 women involved identifying subjects with the C825T genotype using the polymerase chain reaction, followed by restriction fragment-length polymorphism analysis. Overweight was defined as a BMI > or = kg/m(2). Genotype distributions for C825T in overweight men (CC = 80, CT = 162, TT = 80) and women (CC = 52, CT = 91, TT = 40) were not significantly different from normal-weight men (CC = 278, CT = 588, TT = 265) and women (CC = 242, CT = 549, TT = 198). The allele distributions were also not significantly different between either sex. The power of the study was estimated as 98% in men and 81% in women based on the allelic frequencies reported in a previous positive study in Chinese subjects. Multiple logistic regression analysis showed that the genotype was not significantly associated with overweight. In conclusion, this study indicated that the GNB3 C825T polymorphism is not a significant factor for overweight in Japanese people.
authors.affiliation: Department of Occupational and Environmental Medicine, Graduate School of Medicine, Chiba University, Chiba, Japan. suwa@faculty.chiba-u.jp
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Suwazono
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Okubo
authors.forename: Etsuko
authors.initials: E
authors.lastname: Kobayashi
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Miura
authors.forename: Yuko
authors.initials: Y
authors.lastname: Morikawa
authors.forename: Masao
authors.initials: M
authors.lastname: Ishizaki
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Kido
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nakagawa
authors.forename: Koji
authors.initials: K
authors.lastname: Nogawa
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D015992:Body Mass Index; D003430:Cross-Sectional Studies; D005260:Female; D005838:Genotype; D020962:Heterotrimeric GTP-Binding Proteins / Q000235:genetics*; D006801:Humans; D007564:Japan; D008019:Life Style; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
chemical_list: C454420:G-protein beta3 subunit; D020962:Heterotrimeric GTP-Binding Proteins