The effect of taurine on cholesterol degradation in mice fed a high-cholesterol diet.
The hypocholesterolemic effect of taurine was examined in mice fed a high-cholesterol diet containing 1% cholesterol and 0.25% sodium cholate. Male C57BL/6 mice were divided into 3 groups: control group (HC), 1% taurine-supplemented group (HCT+), and taurine-deficient group (HCT-) produced by supplying 0.5% guanidinoethyl sulfonate (GES) solution ad libitum instead of water. After they were fed with the respective diet or drinking water for 4 weeks, the liver taurine level was reduced 80% in the HCT- group compared with that in the HC group, although there was no difference in the serum taurine amount between the two groups. The formation ratio of cholesterol gallstones increased from 71% to 100% by taurine deficiency, and decreased to 0% by taurine supplementation. Compared with the HC group, serum and liver cholesterol significantly decreased, and the excretion of fecal bile acid notably rose in the HCT+ group but tended to lower in the HCT- group. There were no differences in LDL receptor protein level among the three groups. In the subsequent experiment, triglycerides (TG) secretion rate was determined and found to be significantly suppressed by taurine supplementation. In conclusion, it is suggested that taurine does not up-regulate LDL receptor protein level, and the decrease in cholesterol in the circulation is mainly due to its suppressive effect on TG secretion from the liver.
Chen
Wen
W
Laboratory of Nutritional Biochemistry, School of Food and Nutritional Sciences, The University of Shizuoka, Shizuoka 422-8526, Japan. chenwenshizuoka@yahoo.com
Matuda
Keiko
K
Nishimura
Naomichi
N
Yokogoshi
Hidehiko
H
D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D002784:Cholesterol / Q000097:blood / Q000378:metabolism*; D002791:Cholesterol, Dietary; D042882:Gallstones / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011973:Receptors, LDL / Q000378:metabolism; D013654:Taurine / Q000097:blood / Q000378:metabolism / Q000494:pharmacology*; D014280:Triglycerides / Q000378:metabolism
D002791:Cholesterol, Dietary; D011973:Receptors, LDL; D014280:Triglycerides; D013654:Taurine; D002784:Cholesterol