title: Antiproliferative activity of Copaifera duckei oleoresin on liver regeneration in rats.
abstract: Copaiba oleoresin is widely used in folk medicine as an antiinflammatory, healing and antiseptic agent. Wistar rats received Copaifera duckei oleoresin (CDO) or saline solution (SS) from 7 days before surgery until killing. Partial hepatectomy (PH) or sham-operation was performed. Hepatocellular proliferation and liver mitochondrial respiration were evaluated 24 h after the procedure. The proliferating cell nuclear antigen labelling index was significantly lower in CDO-treated plus PH rats than in SS-treated plus PH rats, suggesting that CDO inhibited cell proliferation. The state 3 and state 4 respiration rates were greater for CDO-treated groups, and the stimulation of state 4 respiration was more expressive. These data resulted in a significant decrease in the respiratory control ratio observed for CDO-treated groups, demonstrating the ability of CDO to uncouple oxidative phosphorylation in mitochondria, a fact that may be one explanation for the inhibition of hepatocellular proliferation observed in the CDO-treated plus PH group.
authors.affiliation: Faculty of Medicine of Ribeirão Preto, University of São Paulo, São Paulo, Brazil. orlandocsj@hotmail.com
authors.forename: Orlando
authors.initials: O
authors.lastname: Castro-E-Silva
authors.forename: Sérgio
authors.initials: S
authors.lastname: Zucoloto
authors.forename: Fernando S
authors.initials: FS
authors.lastname: Ramalho
authors.forename: Leandra N Z
authors.initials: LN
authors.lastname: Ramalho
authors.forename: José M C
authors.initials: JM
authors.lastname: Reis
authors.forename: Alvaro A C
authors.initials: AA
authors.lastname: Bastos
authors.forename: Marcus V H
authors.initials: MV
authors.lastname: Brito
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D002455:Cell Division / Q000187:drug effects; D007887:Fabaceae; D006498:Hepatectomy; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; C018620:oleoresins