title: Effect of congestive heart failure on humoral and ex vivo cellular immune responses to influenza vaccination in older adults.
abstract: This study examined the effect of congestive heart failure (CHF) on immune responses to influenza vaccination (2000-2001 preparation) in three groups of older adults including healthy, Class II and Class III/IV CHF. Serum antibody titers measured by hemagglutination inhibition (HI), and interferon-gamma (IFN-gamma), interleukin-10 (IL-10) and granzyme B (GrzB) levels in ex vivo virus-activated mononuclear cell cultures showed significant responses from pre-vaccination to 4 and 12 weeks post-vaccination (P<0.01). There was a trend for lower GrzB and higher IFN-gamma and IL-10 levels in healthy versus CHF groups (P<0.06) for all viral strains at 4 weeks. HI titers did not differ between groups. In the regression model, Grz B levels were significantly predicted by the IFN-gamma:IL-10 ratio and performance on the 6 min Walk Test; age and CHF dropped out of the model. In conclusion, CHF in older adults predicts GrzB responses to influenza vaccination due to cytokine and physical ability differences.
authors.affiliation: Center for Immunotherapy of Cancer and Infectious Diseases, MC 1601, University of Connecticut, School of Medicine, 263 Farmington Avenue, Farmington, CT 06030-1601, USA. jmcelhaney@uchc.edu
authors.forename: Janet E
authors.initials: JE
authors.lastname: McElhaney
authors.forename: John M
authors.initials: JM
authors.lastname: Herre
authors.forename: M Louise
authors.initials: ML
authors.lastname: Lawson
authors.forename: Sharon K
authors.initials: SK
authors.lastname: Cole
authors.forename: Bonnie L
authors.initials: BL
authors.lastname: Burke
authors.forename: Jonathan W
authors.initials: JW
authors.lastname: Hooton
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis; D000917:Antibody Formation / Q000276:immunology* / Q000502:physiology*; D005080:Exercise Test; D005260:Female; D053804:Granzymes; D006333:Heart Failure / Q000276:immunology* / Q000503:physiopathology; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology* / Q000502:physiology*; D007252:Influenza Vaccines / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology / Q000517:prevention & control; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D012044:Regression Analysis; D012697:Serine Endopeptidases / Q000032:analysis / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014611:Vaccination
chemical_list: D000914:Antibodies, Viral; D007252:Influenza Vaccines; D016753:Interleukin-10; D007371:Interferon-gamma; C509213:GZMB protein, human; D053804:Granzymes; D012697:Serine Endopeptidases