Simultaneous determination of twelve inorganic and organic arsenic compounds by liquid chromatography-ultraviolet irradiation-hydride generation atomic fluorescence spectrometry.
A coupling between column liquid chromatography (LC) and atomic fluorescence spectrometry was developed for arsenic speciation. After separation, the compounds are oxidised on-line by UV irradiation, volatilised by hydride-generation and carried to the detector by a stream of argon. A combination of anion-exchange and hydrophobic interactions in a single column (Dionex AS7) was found suitable for the simultaneous separation of organic and inorganic species. Twelve compounds (arsenite, arsenate, monomethylarsonic acid, dimethylarsinic acid, arsenobetaine, arsenocholine, trimethylarsine oxide, tetramethylarsonium ion and four arsenosugars) were separated using an acetate buffer and a nitric acid solution as mobile phases. Limits of detection are 4-22 pg. The technique was applied to three marine samples. Arsenobetaine was detected as the main species in all samples, with concentrations varying from 59 to 1947 ng(As) g(-1) of fresh mass.
Simon
Stéphane
S
Laboratoire de Chimie Analytique Bio-Inorganique et Environnement, Université de Pau et des Pays de l' Adour, UMR 5034, Pau 64000, France.
Tran
Huong
H
Pannier
Florence
F
Potin-Gautier
Martine
M
D000818:Animals; D001152:Arsenicals / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D003445:Crustacea; D006859:Hydrogen / Q000737:chemistry*; D007287:Inorganic Chemicals / Q000032:analysis*; D009930:Organic Chemicals / Q000032:analysis*; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014466:Ultraviolet Rays
D001152:Arsenicals; D007287:Inorganic Chemicals; D009930:Organic Chemicals; D006859:Hydrogen