title: Site-directed mutagenesis of the Saccharomyces cerevisiae dihydropteroate synthase FOL1 gene to study Pneumocystis jirovecii mutations in the orthologue gene FAS.
authors.affiliation: Microbiology Institute, University Hospital, 1011 Lausanne, Switzerland.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Meneau
authors.forename: Dominique
authors.initials: D
authors.lastname: Sanglard
authors.forename: Jacques
authors.initials: J
authors.lastname: Bille
authors.forename: Philippe M
authors.initials: PM
authors.lastname: Hauser
mesh_terms: D003001:Cloning, Molecular; D003470:Culture Media; D004094:Dihydropteroate Synthase / Q000235:genetics* / Q000378:metabolism; D005784:Gene Amplification; D016297:Mutagenesis, Site-Directed; D045363:Pneumocystis carinii / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
chemical_list: D003470:Culture Media; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D004094:Dihydropteroate Synthase