Role of chemical interactions in bacterial adhesion to polymer surfaces.
Development of biomaterial-related infections is attracting an increasing interest due to the significant percentage of implant failure in the hospital care. Recent literature puts in evidence the dependence of the infection risk on the different biomaterials used, because of the different interactions between material surface and micro-organisms. Despite this, the mechanisms underlying the adhesion of bacteria to the biomaterial surface are still unclear. Aim of this work is to study the initial events of the processes responsible for the bacterial adhesion on polymers in order to prevent the development of bacterial infections and the consequent failure and replacement of biomedical devices. Electrostatic and Lifshitz-van der Waals forces are usually considered responsible for the interactions at the biomaterial interface. A new term that involves Lewis acid-base interactions is here introduced to better describe the bacterial adhesion to the polymer surface. Two requirements are needed to test this hypothesis: the development of an ideal polymeric surface in terms of chemical and morphological properties and the choice of a specific bacterial strain to be utilized as "probe". Experiments were worked out using an Escherichia coli (Gram-) strain that represent one of the principal isolates from infected biomaterial implants and its adhesion was investigated on polymers having different acid/basic character. The findings indicate that the bacterial adhesion is influenced by the chemical properties of the polymeric surface. These results may be interpreted taking into account a mechanism in which the acid/base (Lewis) interaction plays an important role.
Speranza
G
G
ITC-IRST, Centro per la Ricerca Scientifica e Tecnologica, Sommarive str 18, 38050 Povo, Trento, Italy. speranza@itc.it
Gottardi
G
G
Pederzolli
C
C
Lunelli
L
L
Canteri
R
R
Pasquardini
L
L
Carli
E
E
Lui
A
A
Maniglio
D
D
Brugnara
M
M
Anderle
M
M
D001422:Bacterial Adhesion; D001672:Biocompatible Materials / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry* / Q000166:cytology* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing / Q000379:methods; D020959:Polyethylene / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D011143:Polyvinyl Chloride / Q000737:chemistry*; D013499:Surface Properties
D001672:Biocompatible Materials; D011108:Polymers; D011143:Polyvinyl Chloride; D020959:Polyethylene; D019904:Polymethyl Methacrylate