[Histomorphologic and salivary gland scintigraphic findings in radiation-induced sialadenitis due to fractionated irradiation of the head and neck region of rats. A model for evaluating potentially radioprotective substances].
BACKGROUND
The aim of this study was to correlate structural, histomorphological damage of the salivary gland with scintigraphic findings during fractioned radiotherapy.


METHODS
The head and neck area of 27 WAG/RijH rats was irradiated with (60)Co-gamma-rays (60 Gy/30f/6 weeks). A port-system was implanted and (99m)Tc-pertechnetat applied at different stages of irradiation (0, 16, 30, 46, 60 Gy and 6 months post irradiation).


RESULTS
After the application of 16 Gy an intra- and extra-cellular oedema developed in the salivary glands. The progressive vacuolisation (30 Gy) passed over into lipomatosis (46 Gy) and necrosis (60 Gy) in the parotid and mandibular glands. Six months after irradiation treatment, the chronic histomorphological damage corresponded to stage II according to Seifert. The corresponding loss in gland function was 13% (16 Gy); 26% (30 Gy); 57% (46 Gy); 75% (60 Gy) and 66.5% (6 months post irradiation).


CONCLUSIONS
This animal model demonstrates the correlation between histomorphological and scintigraphic findings.
Sagowski
C
C
Klinik und Poliklinik für Hals-, Nasen- und Ohrenheilkunde, Universitätsklinikum Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg. sagowski@uke.uni-hamburg.de
Wenzel
S
S
Riehle
I
I
Tesche
S
S
Jenicke
L
L
Zywietz
F
F
Roeser
K
K
Metternich
F U
FU
D000818:Animals; D003037:Cobalt Radioisotopes; D004195:Disease Models, Animal; D019583:Dose Fractionation, Radiation; D004307:Dose-Response Relationship, Radiation; D008068:Lipomatosis / Q000473:pathology; D008297:Male; D010306:Parotid Gland / Q000000981:diagnostic imaging / Q000473:pathology / Q000528:radiation effects; D011833:Radiation Injuries, Experimental / Q000000981:diagnostic imaging* / Q000473:pathology; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D011867:Radioisotope Teletherapy / Q000009:adverse effects*; D011877:Radionuclide Imaging; D051381:Rats; D011919:Rats, Inbred Strains; D012469:Salivary Glands / Q000000981:diagnostic imaging / Q000473:pathology / Q000528:radiation effects*; D013670:Sodium Pertechnetate Tc 99m; D013223:Statistics as Topic; D013363:Submandibular Gland / Q000000981:diagnostic imaging / Q000473:pathology / Q000528:radiation effects
D003037:Cobalt Radioisotopes; D011837:Radiation-Protective Agents; D013670:Sodium Pertechnetate Tc 99m