Simultaneous determination of vitamin E homologs in chicken meat by liquid chromatography with fluorescence detection.
A simple and reliable method for the simultaneous determination of all eight homologs of Vitamin E in chicken meat is described. All analytes, including the internal standard (alpha-tocopherol acetate), were eluted within 35 min and detected using their native fluorescence (295 nm excitation and 330 nm emission). Chromatography using hexane based eluent on a normal phase silica column included an initial column conditioning step to prevent irreversible adsorption of tocopherols and tocotrienols on silica. Lowest detectable levels of alpha-tocopherol, gamma-tocopherol, alpha-tocotrienol, beta-tocotrienol, gamma-tocotrienol and delta-tocotrienol were 0.73, 0.86, 1.0, 1.2, 1.7 and 1.3 ng, respectively.
Hewavitharana
A K
AK
CSIRO, Food Science Australia, Cannon Hill, Qld. 4170, Australia. Amitha_Hewavitharana@health.qld.gov.au
Lanari
M C
MC
Becu
C
C
D000818:Animals; D002645:Chickens; D002853:Chromatography, Liquid / Q000379:methods*; D011202:Poultry Products / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014810:Vitamin E / Q000032:analysis*
D014810:Vitamin E