Recombinant endo-beta-1,4-xylanase from Penicillium canescens.
Recombinant endo-beta-1,4-xylanase (Xyl-31rec, 31 kD, pI 8.2-9.3, the tenth family of glycosyl hydrolases) was isolated from the culture liquid of Penicillium canescens (strain with the amplified homologous xylanase gene) by chromatofocusing on Mono P and hydrophobic chromatography on phenyl-Superose. It is shown that the biochemical and kinetic parameters, substrate specificity, stability, and other properties of the recombinant and native enzymes are almost the same. It was found that Xyl-31rec can be used for biobleaching of cellulose, the recombinant P. canescens strains providing a high yield of extracellular Xyl-31rec (up to 800-900 U/ml of culture liquid) and not secreting cellulases.
Sinitsyna
O A
OA
Faculty of Chemistry, Lomonosov Moscow State University, Moscow 199899, Russia. oasinitsyna@enzyme.chem.msu.ru
Gusakov
A V
AV
Okunev
O N
ON
Serebryany
V A
VA
Vavilova
E A
EA
Vinetsky
Yu P
YP
Sinitsyn
A P
AP
D000042:Absorption; D002482:Cellulose / Q000378:metabolism; D002845:Chromatography; D043364:Endo-1,4-beta Xylanases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature
D011994:Recombinant Proteins; D002482:Cellulose; D043364:Endo-1,4-beta Xylanases