title: Role of LBPA and Alix in multivesicular liposome formation and endosome organization.
abstract: What are the components that control the assembly of subcellular organelles in eukaryotic cells? Although membranes can clearly be distorted by cytosolic factors, very little is known about the intrinsic mechanisms that control the biogenesis, shape, and organization of organellar membranes. Here, we found that the unconventional phospholipid lysobisphosphatidic acid (LBPA) could induce the formation of multivesicular liposomes that resembled the multivesicular endosomes that exist where this lipid is found in vivo. This process depended on the same pH gradient that exists across endosome membranes in vivo and was selectively controlled by Alix. In turn, Alix regulated the organization of LBPA-containing endosomes in vivo.
authors.affiliation: Department of Biochemistry, University of Geneva, 30 quai Ernest Ansermet, 1211 Geneva 4, Switzerland.
authors.forename: Hirotami
authors.initials: H
authors.lastname: Matsuo
authors.forename: Julien
authors.initials: J
authors.lastname: Chevallier
authors.forename: Nathalie
authors.initials: N
authors.lastname: Mayran
authors.forename: Isabelle
authors.initials: I
authors.lastname: Le Blanc
authors.forename: Charles
authors.initials: C
authors.lastname: Ferguson
authors.forename: Julien
authors.initials: J
authors.lastname: Fauré
authors.forename: Nathalie Sartori
authors.initials: NS
authors.lastname: Blanc
authors.forename: Stefan
authors.initials: S
authors.lastname: Matile
authors.forename: Jacques
authors.initials: J
authors.lastname: Dubochet
authors.forename: Rémy
authors.initials: R
authors.lastname: Sadoul
authors.forename: Robert G
authors.initials: RG
authors.lastname: Parton
authors.forename: Francis
authors.initials: F
authors.lastname: Vilbois
authors.forename: Jean
authors.initials: J
authors.lastname: Gruenberg
mesh_terms: D000818:Animals; D017306:Annexin A2 / Q000378:metabolism; D001190:Arylsulfonates / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins; D002460:Cell Line; D004396:Coloring Agents / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004705:Endocytosis; D056827:Endosomal Sorting Complexes Required for Transport; D011992:Endosomes / Q000378:metabolism* / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008051:Lipid Bilayers; D008081:Liposomes / Q000378:metabolism*; D008246:Lysophospholipids / Q000737:chemistry / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism; D015394:Molecular Structure; D050178:Monoglycerides; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology; D014759:Viral Envelope Proteins / Q000378:metabolism
chemical_list: D017306:Annexin A2; D001190:Arylsulfonates; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D004396:Coloring Agents; D056827:Endosomal Sorting Complexes Required for Transport; C028391:G protein, vesicular stomatitis virus; D008051:Lipid Bilayers; D008081:Liposomes; D008246:Lysophospholipids; D008562:Membrane Glycoproteins; D050178:Monoglycerides; C117020:PDCD6IP protein, human; D034741:RNA, Small Interfering; D014759:Viral Envelope Proteins; C012786:bis(monoacylglyceryl)phosphate; C005047:pyranine