Induction of CD8+ T cells to an HIV-1 antigen upon oral immunization of mice with a simian E1-deleted adenoviral vector.
An E1-deleted adenoviral recombinant derived from the chimpanzee serotype 6 expressing a codon-optimized truncated form of gag of human immunodeficiency virus type 1 (HIV-1) was tested for induction of a transgene product-specific CD8+ T cell response upon oral immunization of mice. The vector was shown to induce gag-specific CD8+ T cells detectable at moderate frequencies of approximately 0.5-1.0% in the spleens and to provide partial protection in a surrogate challenge model based on intraperitoneal (i.p.) infection of mice with a vaccinia virus recombinant expressing gag (VVgag) of HIV-1. Frequencies of gag-specific CD8+ T cells could be augmented by using a different, i.e., heterologous, vaccine carrier based on a distinct recombinant virus or an alternative adenoviral serotype expressing the same form of gag for oral or systemic-booster immunization.
Pinto
A R
AR
The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104-4268, USA.
Fitzgerald
J C
JC
Gao
G P
GP
Wilson
J M
JM
Ertl
H C J
HC
D000261:Adenoviruses, Simian / Q000235:genetics*; D000284:Administration, Oral; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D015683:Gene Products, gag / Q000276:immunology; D005822:Genetic Vectors; D015488:HIV Antigens / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006367:HeLa Cells; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology*; D007114:Immunization; D007117:Immunization, Secondary; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology; D014616:Vaccinia virus / Q000276:immunology
D016207:Cytokines; D015683:Gene Products, gag; D015488:HIV Antigens; D010455:Peptides; D014614:Vaccines, Synthetic