title: Absence of mechanical allodynia and Abeta-fiber sprouting after sciatic nerve injury in mice lacking membrane-type 5 matrix metalloproteinase.
abstract: Matrix metalloproteinases (MMPs) are a family of endopeptidases that degrade extracellular matrix components. Membrane-type 5 MMP (MT5-MMP/MMP-24) was identified as neuron-specific, and is believed to contribute to neuronal circuit formation and plasticity. To elucidate its function in vivo, we have generated mice lacking MT5-MMP by gene targeting. MT5-MMP-deficient mice were born without obvious morphological abnormalities. No apparent histological defects were observed in the nervous system either. However, MT5-MMP-deficient mice did not develop neuropathic pain with mechanical allodynia after sciatic nerve injury, though responses to acute noxious stimuli were normal. Neuropathic pain induced by peripheral nerve lesions is known to accompany structural reorganization of the nervous system. Intraneural injection of cholera toxin B subunit, a transganglionic tracer, into the injured sciatic nerve of wild-type mice revealed that the myelinated Abeta-fiber primary afferents sprouted from laminae III-VI of the dorsal horn of the spinal cord and invaded lamina II. However, no such sprouting and invasion of Abeta-fibers were observed in MT5-MMP-deficient mice. These findings suggest that MT5-MMP is essential for the development of mechanical allodynia and plays an important role in neuronal plasticity in this mouse model.
authors.affiliation: Department of Cancer Cell Research, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokane-dai, Minato-ku, Tokyo 108-8639, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Komori
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nonaka
authors.forename: Akiko
authors.initials: A
authors.lastname: Okada
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kinoh
authors.forename: Hiromi
authors.initials: H
authors.lastname: Hayashita-Kinoh
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Yoshida
authors.forename: Ikuo
authors.initials: I
authors.lastname: Yana
authors.forename: Motoharu
authors.initials: M
authors.lastname: Seiki
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D015698:Genomic Library; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009437:Neuralgia / Q000235:genetics*; D009474:Neurons / Q000201:enzymology* / Q000502:physiology; D012584:Sciatic Nerve / Q000293:injuries*
chemical_list: D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases; C118502:Mmp24 protein, mouse