title: Crystallization and preliminary X-ray analysis of xylanase B from Clostridium stercorarium.
abstract: Recombinant mature xylanase B from Clostridium stercorarium has been prepared and crystallized by the sitting-drop vapour-diffusion method using 4 mg ml(-1) purified enzyme, 10.3%(w/v) polyethylene glycol 1500, 8.6%(v/v) glycerol and 0.34 M non-detergent sulfobetaine 195. A suitable crystal grew after incubation for ten weeks at 293 K. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 64.76, b = 96.60, c = 138.44 A. X-ray diffraction data were collected to 1.80 A resolution.
authors.affiliation: National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Nishimoto
authors.forename: Shinya
authors.initials: S
authors.lastname: Fushinobu
authors.forename: Akimasa
authors.initials: A
authors.lastname: Miyanaga
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Wakagi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Shoun
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sakka
authors.forename: Kunio
authors.initials: K
authors.lastname: Ohmiya
authors.forename: Satoru
authors.initials: S
authors.lastname: Nirasawa
authors.forename: Motomitsu
authors.initials: M
authors.lastname: Kitaoka
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Hayashi
mesh_terms: D003013:Clostridium / Q000201:enzymology*; D018360:Crystallography, X-Ray; D003902:Detergents / Q000494:pharmacology; D004058:Diffusion; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry*; D004926:Escherichia coli / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D013696:Temperature
chemical_list: D003902:Detergents; D011994:Recombinant Proteins; D011092:Polyethylene Glycols; D043364:Endo-1,4-beta Xylanases