Poly-3-hexyl thiophene Langmuir-Blodgett films for application to glucose biosensor.
Langmuir-Blodgett films of poly(3-hexyl thiophene) have been prepared by simultaneous entrapment of glucose oxidase and transferred onto the indium-tin-oxide coated glass plates. The films have been characterized by FTIR spectroscopy and detailed response studies have been performed with respect to glucose concentration, temperature, and storage time. These P3HT/SA/GOX electrodes have been utilized for glucose estimation from 100-500 mg/dL by amperometric method. The electrodes have been found to have sensitivity of 0.75 nA/mg/dL detection limit of 50 mg/dL and shelf life of about 75 days at 4 degrees C.
Singhal
Rahul
R
Biomolecular Electronics & Conducting Polymer Research Group, National Physical Laboratory, Dr. KS Krishnan Road, New Delhi- 110012, India.
Chaubey
Asha
A
Kaneto
Keiichi
K
Takashima
W
W
Malhotra
B D
BD
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004800:Enzymes, Immobilized; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis* / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D008422:Materials Testing; D008567:Membranes, Artificial; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013876:Thiophenes / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D011108:Polymers; D013876:Thiophenes; C066730:polythiophene; D005949:Glucose Oxidase; D005947:Glucose