title: [Study on the injurious effect of a self designed micro-skin machine on the epithelia].
abstract: OBJECTIVE
  To observe the injury on micro-skin induced by a self designed micro-skin machine.
  
  
  METHODS
  Micro-skin was produced either with the machine or by hand. Cells at the edge of micro-skin were observed by transmission electron microscope. succinic dehydrogenase activity in supernatant of cultivated cells was analyzed, and the cell proliferation of micro-skin was assessed by (3)H-TdR. Twenty patients were enrolled in the study for the observation of the wound healing time between the two groups of micro-skin after being grafted.
  
  
  RESULTS
  Transmission electron microscope examination revealed that the cellular injury at the edge of the micro-skin in machine-made group was mild compared with that in man-made group. (3)H-TdR rate was elevated but the activity of succinic dehydrogenase in the supernatant of cultured cells decreased in supernatant of cultured cells of machine produced micro-skin. Wound healing time was shortened in machine made group. (P < 0.05).
  
  
  CONCLUSIONS
  The cellular injury at the edge of micro-skin in the machine made group was mild when compared with that in the man-made group with cell proliferation accelerated and wound healing time shortened.
authors.affiliation: Department of Burns, The 187th Hospital of PLA, Haikou 571159, Hainan Province, P.R. China.
authors.forename: Jian-she
authors.initials: JS
authors.lastname: Chen
authors.forename: Jin-song
authors.initials: JS
authors.lastname: Chen
authors.forename: Xian-zhi
authors.initials: XZ
authors.lastname: Liu
authors.forename: Zong-ren
authors.initials: ZR
authors.lastname: Zhang
authors.forename: Guang-yu
authors.initials: GY
authors.lastname: Shen
authors.forename: Hong-jie
authors.initials: HJ
authors.lastname: Duan
authors.forename: Yong-yue
authors.initials: YY
authors.lastname: Su
authors.forename: Yue-ming
authors.initials: YM
authors.lastname: Liu
authors.forename: Gen-fa
authors.initials: GF
authors.lastname: Lv
mesh_terms: D002056:Burns / Q000601:surgery*; D002455:Cell Division; D004848:Epithelium / Q000473:pathology; D006801:Humans; D008854:Microscopy, Electron; D012867:Skin / Q000648:ultrastructure; D016038:Skin Transplantation / Q000379:methods*; D014945:Wound Healing