title: Thioglycosynthases: double mutant glycosidases that serve as scaffolds for thioglycoside synthesis.
abstract: A double mutant, retaining glycosidase that lacks both the catalytic nucleophile and the catalytic acid/base residues efficiently catalyzes thioglycoside formation from a glycosyl fluoride donor and thiosugar acceptors.
authors.affiliation: Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, B.C. V6T 1Z1, Canada.
authors.forename: Michael
authors.initials: M
authors.lastname: Jahn
authors.forename: Hongming
authors.initials: H
authors.lastname: Chen
authors.forename: Johannes
authors.initials: J
authors.lastname: Müllegger
authors.forename: Jennifer
authors.initials: J
authors.lastname: Marles
authors.forename: R Antony J
authors.initials: RA
authors.lastname: Warren
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Withers
mesh_terms: D002942:Circular Dichroism; D004795:Enzyme Stability; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D009154:Mutation / Q000235:genetics*; D013696:Temperature; D013865:Thioglycosides / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D013865:Thioglycosides; D006026:Glycoside Hydrolases