PriA is essential for viability of the Escherichia coli topoisomerase IV parE10(Ts) mutant.
The parE10(Ts) mutation, which renders Escherichia coli thermosensitive for growth by inactivation of the essential E. coli topoisomerase topo IV, is lethal at all temperatures when PriA, the main replication restart protein, is absent. This lethality is suppressed by the activation of a PriA-independent replication restart pathway (dnaC809 mutation). This result suggests that topo IV acts prior to full-chromosome replication completion.
Grompone
Gianfranco
G
Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, 78352 Jouy en Josas, France.
Bidnenko
Vladimir
V
Ehrlich
S Dusko
SD
Michel
Bénédicte
B
D000251:Adenosine Triphosphatases / Q000502:physiology*; D027081:DNA Gyrase / Q000502:physiology; D004265:DNA Helicases / Q000502:physiology*; D004261:DNA Replication; D027101:DNA Topoisomerase IV / Q000502:physiology*; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development*; D029968:Escherichia coli Proteins / Q000502:physiology*; D009154:Mutation; D011938:Rec A Recombinases / Q000378:metabolism
D029968:Escherichia coli Proteins; D011938:Rec A Recombinases; D000251:Adenosine Triphosphatases; C506743:priA protein, E coli; D004265:DNA Helicases; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase