The reproducibility of an enzyme-linked immunosorbent assay for detection of Chlamydia pneumoniae-specific antibodies.
With use of an enzyme-linked immunosorbent assay (ELISA), Chlamydia pneumoniae immunoglobulins were detected in a consecutive series of patients' sera (n = 122 for IgA and IgG; n = 138 for IgM). When the ELISA tests were repeated, the percentage disagreements were 12%, 16% and 10% for C. pneumoniae IgA, IgG and IgM, respectively. The reproducibility of the ELISA, expressed as kappa values, for IgA, IgG and IgM was 0.73, 0.60 and 0.53, respectively (p < 0.001). It was concluded that the ELISA had good reproducibility for detecting C. pneumoniae IgA, and moderately good reproducibility for detecting C. pneumoniae IgG and IgM.
Ngeh
J
J
Department of Medicine for Elderly People, Whipps Cross University Hospital, London, UK. JNgeh@aol.com
Gupta
S
S
Goodbourn
C
C
D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood*; D016022:Case-Control Studies; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000382:microbiology; D016993:Chlamydophila pneumoniae / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015203:Reproducibility of Results; D020521:Stroke / Q000150:complications
D000907:Antibodies, Bacterial