[Isolation and characterization of human placenta derived adherent cells and their hematopoietic growth factor expression].
OBJECTIVE
To isolate and culture human placenta derived adherent cells (hPDAC) and assay their hematopoietic growth factor expression.


METHODS
By enzyme digestion, hPDAC were isolated from human placenta tissue and cultured, and their biological characteristics were studied. The hematopoietic growth factor (HGF) mRNA expression of hPDAC was assayed by RT-PCR.


RESULTS
hPDAC was successfully isolated from human placenta tissue, which was further confirmed as mesenchymal stem cell-like cells. HGF including SCF, FL, G-CSF, GM-CSF, M-CSF and IL-6 were expressed in hPDAC.


CONCLUSIONS
hPDAC could be used as feeder layer for umbilical cord blood CD(34)(+) cells ex vivo expansion.
He
Jin
J
Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
Zhang
Yi
Y
Jiang
Xiao-xia
XX
Liu
Gang
G
Liu
Yuan-lin
YL
Li
He-lian
HL
Mao
Ning
N
D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression; D016179:Granulocyte Colony-Stimulating Factor / Q000235:genetics; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics; D016298:Hematopoietic Cell Growth Factors / Q000235:genetics*; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D010920:Placenta / Q000166:cytology / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019089:Stem Cell Factor / Q000235:genetics
D016298:Hematopoietic Cell Growth Factors; D015850:Interleukin-6; D008565:Membrane Proteins; D012333:RNA, Messenger; D019089:Stem Cell Factor; C084881:flt3 ligand protein; D016179:Granulocyte Colony-Stimulating Factor; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor