Adrenalectomy further suppresses the NT-3 mRNA response to traumatic brain injury but this effect is not reversed with corticosterone.
Fluid percussion injury (FPI) and in situ hybridisation were used to evaluate the expression of NT-3 mRNA in the hippocampus after traumatic brain injury (TBI) in adrenal-intact and adrenalectomised rats (with or without corticosterone replacement). FPI and adrenalectomy independently significantly reduced the expression of NT-3 mRNA in the dentate gyrus (DG) and CA2 region. The effects of adrenalectomy in the CA2 region were partially reversed with corticosterone. In adrenalectomised animals undergoing FPI, a further significant decrease in NT-3 mRNA was observed in the DG, but this was not reversed by corticosterone. Glucocorticoids may, therefore, play a role in the basal regulation of NT-3 in the hippocampus, but the role of glucocorticoids in the modulation of the NT-3 response to TBI is unclear.
Grundy
Paul L
PL
Department of Neurosurgery, Frenchay Hospital, Bristol, BS16 1LE, UK. p.l.grundy@doctors.org.uk
Patel
Nitin
N
Harbuz
Michael S
MS
Lightman
Stafford L
SL
Sharples
Peta M
PM
D000315:Adrenalectomy; D000704:Analysis of Variance; D000818:Animals; D001345:Autoradiography / Q000379:methods; D001930:Brain Injuries / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D003345:Corticosterone / Q000494:pharmacology* / Q000627:therapeutic use; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D017403:In Situ Hybridization; D008297:Male; D020933:Neurotrophin 3 / Q000235:genetics / Q000378:metabolism*; D010474:Percussion / Q000009:adverse effects; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D020933:Neurotrophin 3; D012333:RNA, Messenger; D003345:Corticosterone