title: Characterization of tumour necrosis factor-alpha-related apoptosis-inducing ligand and its receptors in the adult human testis.
abstract: Tumour necrosis factor-alpha-related apoptosis-inducing ligand (TRAIL) is a member of the tumour necrosis factor-alpha (TNF-alpha) family of cytokines which is known to induce apoptosis upon binding to its death domain-containing receptors, DR4/TRAIL-R1 and DR5/TRAIL-R2. Two additional TRAIL receptors, DcR1/TRAIL-R3 and DcR2/TRAIL-R4, lack functional death domains and act as decoy receptors for TRAIL. In this study, the presence of TRAIL and its receptors was investigated by immunohistochemistry in adult human testes. In addition, TRAIL and its receptors were studied in terms of protein and mRNA using western blot analysis and RT-PCR respectively. TRAIL and its receptors were immunodetected according to the different testicular cell types: TRAIL, DR5/TRAIL-R2 and DcR2/TRAIL-R4 were localized in Leydig cells, DR4/TRAIL-R1 was seen in peritubular and Sertoli cells whereas ligand and all receptors were detected in germ cells. Proteins and mRNA corresponding to TRAIL and its receptors were also identified in adult human testes. In conclusion, TRAIL and its receptors DR4/TRAIL-R1, DR5/TRAIL-R2, DcR1/TRAIL-R3 and DcR2/TRAIL-R4 are expressed in the human testis, and are predominantly localized in different germ cell types.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, INSERM U-407, Communications Cellulaires en Biologie de la Reproduction, Faculté de Médecine Lyon-Sud, BP 12, F-69921 Oullins Cedex, France. gratarol@lsgrisn1.univ-lyon1.fr
authors.forename: Renée
authors.initials: R
authors.lastname: Grataroli
authors.forename: David
authors.initials: D
authors.lastname: Vindrieux
authors.forename: Jaqueline
authors.initials: J
authors.lastname: Selva
authors.forename: Carole
authors.initials: C
authors.lastname: Felsenheld
authors.forename: Alain
authors.initials: A
authors.lastname: Ruffion
authors.forename: Myriem
authors.initials: M
authors.lastname: Decaussin
authors.forename: Mohamed
authors.initials: M
authors.lastname: Benahmed
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000502:physiology; D051017:Apoptosis Regulatory Proteins; D058851:GPI-Linked Proteins; D006367:HeLa Cells; D006801:Humans; D007120:Immunochemistry; D007985:Leydig Cells / Q000737:chemistry / Q000166:cytology; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000235:genetics; D008565:Membrane Proteins / Q000032:analysis; D008875:Middle Aged; D012333:RNA, Messenger / Q000737:chemistry; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D018124:Receptors, Tumor Necrosis Factor / Q000032:analysis* / Q000235:genetics; D053320:Receptors, Tumor Necrosis Factor, Member 10c; D012708:Sertoli Cells / Q000737:chemistry / Q000166:cytology; D013087:Spermatids / Q000737:chemistry / Q000166:cytology; D013090:Spermatocytes / Q000737:chemistry / Q000166:cytology; D053221:TNF-Related Apoptosis-Inducing Ligand; D013737:Testis / Q000737:chemistry* / Q000166:cytology; D053319:Tumor Necrosis Factor Decoy Receptors; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000235:genetics
chemical_list: D051017:Apoptosis Regulatory Proteins; D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D018124:Receptors, Tumor Necrosis Factor; D053320:Receptors, Tumor Necrosis Factor, Member 10c; D053221:TNF-Related Apoptosis-Inducing Ligand; C105190:TNFRSF10A protein, human; C505639:TNFRSF10B protein, human; C108661:TNFRSF10C protein, human; C451501:TNFRSF10D protein, human; C505653:TNFSF10 protein, human; D053319:Tumor Necrosis Factor Decoy Receptors; D014409:Tumor Necrosis Factor-alpha