Preparation and physicochemical properties of compression-molded keratin films.
The S-sulfo keratin was extracted from wool and was then spray-dried to give S-sulfo keratin powder. Differential scanning calorimetry analysis showed that the glass transition temperature of S-sulfo keratins became lowered with the increase of moisture content, while perfectly dried S-sulfo keratin powder did not give thermal transition in the temperature range 30-130 degrees C. The compression molding of the S-sulfo keratin powder supplemented with one-tenth weight of water afforded a plastic-like transparent proteinous film above the glass transition temperature. The film obtained from the powder without water addition or compression molded below glass transition temperature partly remained powdery. The film compression molded at 120 degrees C gave the maximum ultimate strength and Young's modulus, 27.8 +/- 2.9 and 1218 +/- 80 MPa, respectively. Obtained film was insoluble and slightly swelled in water, but, in the presence of reducing agent, the film significantly swelled at pH 7.0 and even dissolved at pH 9.0, suggesting the relevance of abundant disulfide linkage. The film supported the mammalian cell adhesion and proliferation, demonstrating the biocompatibility of S-sulfo keratin films.
Katoh
Kazunori
K
Department of Bioapplied Chemistry, Graduate School of Engineering, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan. katkaz@owaritex.jp
Shibayama
Mikio
M
Tanabe
Toshizumi
T
Yamauchi
Kiyoshi
K
D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D004548:Elasticity; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007633:Keratins / Q000138:chemical synthesis / Q000737:chemistry* / Q000648:ultrastructure*; D008420:Manufactured Materials; D008422:Materials Testing; D051379:Mice; D008968:Molecular Conformation; D009997:Osmotic Pressure; D011208:Powders; D011312:Pressure; D013718:Tensile Strength; D014935:Wool / Q000737:chemistry*
D001672:Biocompatible Materials; D011208:Powders; D007633:Keratins