Reversed-phase liquid chromatographic method for the determination of ochratoxin A in wine.
In this paper, we propose a new, rapid, highly sensitive and reproducible RP-HPLC-FLD method for the detection of ochratoxin A (OTA) in wine, by directly injecting the liquid in the chromatographic system without any extraction or clean-up. An alkaline mobile phase (NH4Cl:CH-CN 85:15 (v/v), 20 mM, pH 9.8) was used to obtain a distinct fluorescence enhancement. This improvement allows to reach, without an immunoaffinity clean-up or concentration, a detection limit of 0.05 ng/ml, which is similar to those commonly obtained after immunoaffinity purification and acidic elution. The method was statistically validated and directly applied to a series of wine samples.
Dall'Asta
C
C
Dipartimento di Chimica Organica e Industriale, Università degli Studi di Parma, Parco Area delle Scienze 17/A, I-43100 Parma, Italy.
Galaverna
G
G
Dossena
A
A
Marchelli
R
R
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009793:Ochratoxins / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014920:Wine / Q000032:analysis*
D009793:Ochratoxins; C025589:ochratoxin A