Troponin-T and brain natriuretic peptide as predictors for adriamycin-induced cardiomyopathy in rats.
BACKGROUND
Clinical methods for the early detection of doxorubicine (adriamycin; ADR) -induced cardiotoxicity have not been established. This study prospectively investigated whether atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP) and cardiac troponin T (TnT) are predictors for ADR-induced cardiotoxicity, and examined the correlations between the serum concentrations of these biomarkers and the functional alternations associated with ADR-induced myocardial damage.


RESULTS
Male Wistar rats were injected weekly with 2 mg/kg of ADR via the tail vein for 8 weeks to induce cardiotoxicity. Echocardiograms of each ether anesthetized rat were taken at 6, 8, 10 and 12 weeks after the first administration of ADR, and blood samples collected from the tail vein were used to quantify plasma ANP and BNP, and serum TnT after echocardiography. Plasma BNP and serum TnT significantly increased from 6 to 12 weeks (81.5 to 173.3 pg/ml (p<0.001), <0.01 to 1.09 ng/ml (p<0.05), respectively) with deterioration of left ventricular % fractional shortening (%FS) (58.6% to 36.8%). The %FS significantly correlated with TnT (r=-0.51, p<0.001) and BNP (r=-0.75, p<0.0001); however, the increase of TnT was antecedent to the increase of BNP and the deterioration of %FS.


CONCLUSIONS
Plasma BNP and serum TnT concentrations, especially TnT, measured by this highly sensitive method are useful predictors for ADR-induced cardiomyopathy.
Koh
Eikan
E
Department of Pediatrics, Kanazawa Medical University, Uchinada, Japan.
Nakamura
Tsuneyuki
T
Takahashi
Hiroaki
H
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000097:blood; D015415:Biomarkers / Q000097:blood; D009202:Cardiomyopathies / Q000097:blood* / Q000139:chemically induced / Q000175:diagnosis*; D018450:Disease Progression; D004317:Doxorubicin / Q000009:adverse effects*; D008297:Male; D020097:Natriuretic Peptide, Brain / Q000097:blood*; D011237:Predictive Value of Tests; D051381:Rats; D017208:Rats, Wistar; D020107:Troponin T / Q000097:blood*; D018487:Ventricular Dysfunction, Left
D015415:Biomarkers; D020107:Troponin T; D020097:Natriuretic Peptide, Brain; D004317:Doxorubicin; D009320:Atrial Natriuretic Factor