Recombinant human (rh) stem cell factor and rhIL-4 stimulate differentiation and proliferation of CD3+ cells from umbilical cord blood and CD3+ cells enhance FcepsilonR1 expression on fetal liver-derived mast cells in the presence of rhIL-4.
We previously reported that rhIL-4 induced apoptosis and rhIL-6 mediated protection of human mast cells derived from cord blood mononuclear cells. Based on the result, we attempted to obtain the phenotypes and differentiation of CD3+ cells from cord blood by investigating their cell surface markers in the presence of rhSCF plus rhIL-4. The effect of co-cultured CD3+ cells on fetal liver mast cells (FLMCs) was also determined. Phenotypes from cord blood-derived cells were analyzed by flow cytometry and cell numbers were determined. Fetal liver mast cells were cultured with cord blood-derived cells (mainly CD3+) in the presence of rhSCF and/or rhIL-4 and were analyzed to determine cell number and expression of Kit+ and FcepsilonR1. The percentage of CD3+ cells from cord blood-derived cells on day 0 was about 41 +/- 13.5%, following monocytes and granulocytes. CD3+ cells increased in number (1.5-fold) and purity (90%), whereas other cell types did not survive. More than 60% of CD3+ cells from cord blood at day 0 were CD4(-)CD8-. These double-negative cells dramatically decreased by 1 week of culture, while CD4+CD8+ cells increased in number and purity through 3 weeks of culture, and then decreased as greater numbers of single-positive T cells emerged. We also found that FcepsilonR expression on FLMC increased in the presence of rhIL-4, but was not affected by the T cells that developed from cord blood mononuclear cells. The results indicate that IL-4, a Th2 type cytokine, together with rhSCF, can induce T cell proliferations, differentiation, and maturation from cord blood progenitor cells.
Lee
Eunkyung
E
College of Pharmacy, Yeungnam University, Gyongsan 712-749, South Korea.
Min
Hae-Ki
HK
Oskeritzian
Carole A
CA
Kambe
Naotomo
N
Schwartz
Lawrence B
LB
Wook Chang
Hyeun
H
D017252:CD3 Complex / Q000276:immunology*; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002469:Cell Separation; D002478:Cells, Cultured; D018920:Coculture Techniques; D005312:Fetal Blood / Q000166:cytology*; D005333:Fetus / Q000033:anatomy & histology; D005434:Flow Cytometry; D022781:Hepatocytes / Q000276:immunology*; D006801:Humans; D015847:Interleukin-4 / Q000276:immunology*; D008407:Mast Cells / Q000166:cytology / Q000378:metabolism*; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D017455:Receptors, IgE / Q000378:metabolism*; D011994:Recombinant Proteins / Q000276:immunology*; D019089:Stem Cell Factor / Q000276:immunology*; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D017252:CD3 Complex; D017455:Receptors, IgE; D011994:Recombinant Proteins; D019089:Stem Cell Factor; D015847:Interleukin-4; D019009:Proto-Oncogene Proteins c-kit; D001619:beta-N-Acetylhexosaminidases