title: The effect of medicinal plants of Islamabad and Murree region of Pakistan on insulin secretion from INS-1 cells.
abstract: In vitro testing of the extracts of medicinal plants collected from Islamabad and the Murree region on insulin secretagogue activity was carried out. Dried ethanol extracts of all plants (ZH1-ZH19) were dissolved in ethanol and DMSO, and tested at various concentrations (between 1 and 40 microg/mL) for insulin release from INS-1 cells in the presence of 5.5 mM glucose. Glibenclamide was used as a control. Promising insulin secretagogue activity in various plant extracts at 1, 10, 20 and 40 microg/mL was found, while in some cases a decrease in insulin secretion was also observed. Artemisia roxburghiana, Salvia coccinia and Monstera deliciosa showed insulin secretagogue activity at 1 microg/mL (p < 0.05) while Abies pindrow, Centaurea iberica and Euphorbia helioscopia were active at 10 microg/mL (p < 0.05). Extracts of Bauhinia variegata and Bergenia himalacia showed effects at 20 microg/mL (p < 0.05), and Taraxacum officinale and Viburnum foetens at 40 microg/mL (p < 0.05). Insulin secretagogue activity could not be detected in the extracts of Adhatoda vasica, Cassia fistula, Chrysanthemum leucanthemum, Morus alba, Plectranthus rugosus, Peganum harmala and Olea ferruginea. The results suggest that medicinal plants of Islamabad and the Murree region of Pakistan may be potential natural resources for antidiabetic compounds.
authors.affiliation: Department of Chemistry, Quaid-i-Azam University, Islamabad Pakistan. chem63@yahoo.com
authors.forename: Zakir
authors.initials: Z
authors.lastname: Hussain
authors.forename: Abdul
authors.initials: A
authors.lastname: Waheed
authors.forename: Rizwana Aleem
authors.initials: RA
authors.lastname: Qureshi
authors.forename: Dadu Khan
authors.initials: DK
authors.lastname: Burdi
authors.forename: Eugen J
authors.initials: EJ
authors.lastname: Verspohl
authors.forename: Naeema
authors.initials: N
authors.lastname: Khan
authors.forename: Mashooda
authors.initials: M
authors.lastname: Hasan
mesh_terms: D000818:Animals; D002460:Cell Line / Q000187:drug effects / Q000378:metabolism; D003920:Diabetes Mellitus / Q000517:prevention & control; D004305:Dose-Response Relationship, Drug; D035264:Flowers; D005638:Fruit; D005905:Glyburide / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007340:Insulinoma / Q000473:pathology; D008519:Medicine, Traditional; D010154:Pakistan; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves; D018517:Plant Roots; D010946:Plants, Medicinal; D051381:Rats; D013552:Swine
chemical_list: D007004:Hypoglycemic Agents; D007328:Insulin; D010936:Plant Extracts; D005905:Glyburide