High-throughput classification of images of cells transfected with cDNA clones.
The sequence of the human genome has been determined. The next task is to determine the function of the genes. Classifying cellular forms of proteins encoded by human cDNA clones is a primary step toward understanding the biological role of proteins and their coding genes. We report here our ongoing work on an automatic system to facilitate this classification. Our system handles the transfection, incubation, acquisition of microscopic images of the cells, and the classification of forms there appearing in the images. Our system correctly classified proteins by their forms at a rate of 90% in feasibility studies.
Minamikawa-Tachino
Reiko
R
The Tokyo Metropolitan Institute of Medical Science, Tokyo 113-8613, Japan. tachino@rinshoken.or.jp
Kabuyama
Noriko
N
Gotoh
Toshiyuki
T
Kagei
Seiichiro
S
Naruse
Masatoshi
M
Kisu
Yasutomo
Y
Togashi
Takushi
T
Sugano
Sumio
S
Usami
Hitohide
H
Nomura
Nobuo
N
D000465:Algorithms; D002477:Cells / Q000145:classification; D003001:Cloning, Molecular; D018076:DNA, Complementary; D007091:Image Processing, Computer-Assisted; D011506:Proteins; D014162:Transfection
D018076:DNA, Complementary; D011506:Proteins