[Evaluation of the reliability of three different hepatitis E diagnosis reagents on the clinical acute hepatitis E].
OBJECTIVE
To evaluate the reliability of different hepatitis E diagnosis reagent tests on the acute hepatitis E.


METHODS
Three acute hepatitis E diagnosis tests, E2-IgM (Wantai, China), GL-IgM and GL-IgG (Genelabs, Singapore) were compared for their reliability in a sera panel composed by 273 healthy individuals and 525 hepatitis.


RESULTS
The specificity of E2-IgM on the diagnosis of acute hepatitis E was 100.0%, it was significantly higher than GL-IgM (96.7%) and GL-IgG (85.4%). The sensitivity of E2-IgM and GL-IgG were 97.9% and 93.8% respectively, both significantly higher than GL-IgM (72.9%). Among 65 acute hepatitis cases being positive on GL-IgM test but negative on E2-IgM, 58 (89.2%) cases were found to be positive with anti-hepatitis A virus IgM, it indicated that the GL-IgM test might be interfered by other IgM antibodies on serum.


CONCLUSIONS
E2-IgM is a good test for the diagnosis of acute hepatitis E.
Zheng
Ying-jie
YJ
The Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, Xiamen University, Xiamen 361005, China.
Jiang
Qing-wu
QW
Zhang
Jun
J
Zhao
Qi
Q
Ge
Sheng-xiang
SX
Gao
Mei-yang
MY
Lin
Jian
J
Ou
Shang-hai
SH
Xia
Ning-shao
NS
D000208:Acute Disease; D006508:Hepatitis Antibodies / Q000097:blood; D016751:Hepatitis E / Q000175:diagnosis*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D006508:Hepatitis Antibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic