title: Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus bataviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A grasslands.
abstract: A group of 42 isolates were isolated from the soil of several disused hay fields, in the Drentse A agricultural research area (The Netherlands), that were taken out of production at different times. The group represents hitherto-uncultured Bacillus lineages that have previously been found, by a non-cultural method, to be predominant in soil. The strains were subjected to a polyphasic taxonomic study, including (GTG)5-PCR, 16S rDNA sequence analysis, DNA-DNA hybridizations, DNA base-ratio determination, fatty acid analysis and morphological and biochemical characterization. By comparing the groupings obtained by (GTG)5-PCR and 16S rDNA sequence analysis, six clusters of similar strains could be recognized. A DNA-DNA relatedness study showed that these clusters represented five novel genospecies. Further analysis supported the proposal of five novel species in the genus Bacillus, namely Bacillus novalis sp. nov. (type strain IDA3307T=R-15439T=LMG 21837T=DSM 15603T), Bacillus vireti sp. nov. (type strain IDA3632T=R-15447T=LMG 21834T=DSM 15602T), Bacillus soli sp. nov. (type strain IDA0086T=R-16300T=LMG 21838T=DSM 15604T), Bacillus bataviensis sp. nov. (type strain IDA1115T=R-16315T=LMG 21833T=DSM 15601T) and Bacillus drentensis sp. nov. (type strain IDA1967T=R-16337T=LMG 21831T=DSM 15600T).
authors.affiliation: Ghent University, Department BFM (WE10V), Laboratory of Microbiology, K.-L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Jeroen
authors.initials: J
authors.lastname: Heyrman
authors.affiliation: Ghent University, Department BFM (WE10V), Laboratory of Microbiology, K.-L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Bram
authors.initials: B
authors.lastname: Vanparys
authors.affiliation: School of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 0BA, UK.
authors.forename: Niall A
authors.initials: NA
authors.lastname: Logan
authors.affiliation: Ghent University, Department BFM (WE10V), Laboratory of Microbiology, K.-L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: An
authors.initials: A
authors.lastname: Balcaen
authors.affiliation: School of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 0BA, UK.
authors.forename: Marina
authors.initials: M
authors.lastname: Rodríguez-Díaz
authors.affiliation: GBF (German Research Centre for Biotechnology), Division of Microbiology, Mascheroder Weg 1, D-38124 Braunschweig, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Felske
authors.affiliation: Ghent University, Department BFM (WE10V), Laboratory of Microbiology, K.-L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Paul
authors.initials: P
authors.lastname: De Vos
mesh_terms: D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002980:Climate; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005858:Germany; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D006109:Poaceae; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S