Determination of Astragaloside IV in rat plasma by liquid chromatography electrospray ionization mass spectrometry.
Astragaloside IV (AGS-IV) is an active constituent of Radix Astragali used in many Traditional Chinese Medicines. This paper describes a sensitive and specific assay for the quantitation of AGS-IV in rat plasma. After solid phase extraction (SPE), samples were analyzed by liquid chromatography electrospray ionization mass spectrometry using a reversed-phase C18 column. The assay was linear in the range 1-500 ng/ml with a limit of detection of 0.5 ng/ml. The recovery was 92.5% and within-day and between-day precision were 3.7-6.0 and 2.8-9.8%, respectively. The assay was applied to a pharmacokinetic study in rat after a single oral dose. The drug was rapidly absorbed and subsequently eliminated according to a biphasic concentration-time curve.
Gu
Yongchuan
Y
Center of Pharmacokinetics, China Pharmaceutical University, Nanjing, China. njguyc@hotmail.com
Wang
Guangji
G
Fawcett
J Paul
JP
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D014315:Triterpenes / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics
D004365:Drugs, Chinese Herbal; D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A