title: Apoptosis of human gastric adenocarcinoma cells induced by beta-ionone.
abstract: OBJECTIVE
  To investigate the effect of beta-ionone on the growth and apoptosis of gastric adenocarcinoma cell line SGC-7901.
  
  
  METHODS
  Using MTT, fluorescence dye (Hoechst-33258), transmission electron microscopy and the TUNEL assay, we examined growth and apoptosis of SGC-7901 cells treated with beta-ionone at various concentrations (i.e. 25, 50, 100 and 200 micromol/L) for 24 h, 48 h.
  
  
  RESULTS
  The growth of SGC-7901 cells was inhibited by beta-ionone. Seven days after treatment with beta-ionone at four concentrations, the inhibition rates were 12.04%, 30.59%, 78.25% and 94.15%, respectively. The IC(50) value of beta-ionone for SGC-7901 cells was estimated to be 89 micromol/L. The apoptotic morphology was demonstrated in SGC-7901 cells treated with beta-ionone by Hoechst-33258 staining and electron microscopy. Apoptosis was also shown in beta-ionone-treated SGC-7901 cells by the TUNEL assay.
  
  
  CONCLUSIONS
  beta-ionone can inhibit cell proliferation and induce apoptosis of SGC-7901 cells. However, the mechanism needs to be further investigated.
authors.affiliation: Public Health College, Harbin Medical University, Harbin 15001, Heilongjiang Province, China. jiarl@ems.hrbmu.edu.cn
authors.forename: Jia-Ren
authors.initials: JR
authors.lastname: Liu
authors.forename: Bing-Qing
authors.initials: BQ
authors.lastname: Chen
authors.forename: Bao-Feng
authors.initials: BF
authors.lastname: Yang
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Dong
authors.forename: Chang-Hao
authors.initials: CH
authors.lastname: Sun
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Guo
authors.initials: G
authors.lastname: Song
authors.forename: You-Qiang
authors.initials: YQ
authors.lastname: Song
mesh_terms: D000230:Adenocarcinoma / Q000503:physiopathology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D006801:Humans; D045792:Norisoprenoids / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000503:physiopathology*
chemical_list: D045792:Norisoprenoids; C008157:beta-ionone