The effect of medicinal plants of Islamabad and Murree region of Pakistan on insulin secretion from INS-1 cells.
In vitro testing of the extracts of medicinal plants collected from Islamabad and the Murree region on insulin secretagogue activity was carried out. Dried ethanol extracts of all plants (ZH1-ZH19) were dissolved in ethanol and DMSO, and tested at various concentrations (between 1 and 40 microg/mL) for insulin release from INS-1 cells in the presence of 5.5 mM glucose. Glibenclamide was used as a control. Promising insulin secretagogue activity in various plant extracts at 1, 10, 20 and 40 microg/mL was found, while in some cases a decrease in insulin secretion was also observed. Artemisia roxburghiana, Salvia coccinia and Monstera deliciosa showed insulin secretagogue activity at 1 microg/mL (p < 0.05) while Abies pindrow, Centaurea iberica and Euphorbia helioscopia were active at 10 microg/mL (p < 0.05). Extracts of Bauhinia variegata and Bergenia himalacia showed effects at 20 microg/mL (p < 0.05), and Taraxacum officinale and Viburnum foetens at 40 microg/mL (p < 0.05). Insulin secretagogue activity could not be detected in the extracts of Adhatoda vasica, Cassia fistula, Chrysanthemum leucanthemum, Morus alba, Plectranthus rugosus, Peganum harmala and Olea ferruginea. The results suggest that medicinal plants of Islamabad and the Murree region of Pakistan may be potential natural resources for antidiabetic compounds.
Hussain
Zakir
Z
Department of Chemistry, Quaid-i-Azam University, Islamabad Pakistan. chem63@yahoo.com
Waheed
Abdul
A
Qureshi
Rizwana Aleem
RA
Burdi
Dadu Khan
DK
Verspohl
Eugen J
EJ
Khan
Naeema
N
Hasan
Mashooda
M
D000818:Animals; D002460:Cell Line / Q000187:drug effects / Q000378:metabolism; D003920:Diabetes Mellitus / Q000517:prevention & control; D004305:Dose-Response Relationship, Drug; D035264:Flowers; D005638:Fruit; D005905:Glyburide / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007340:Insulinoma / Q000473:pathology; D008519:Medicine, Traditional; D010154:Pakistan; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves; D018517:Plant Roots; D010946:Plants, Medicinal; D051381:Rats; D013552:Swine
D007004:Hypoglycemic Agents; D007328:Insulin; D010936:Plant Extracts; D005905:Glyburide