MTS-conjugated-antiactive caspase 3 antibodies inhibit actinomycin D-induced apoptosis.
Caspase 3 is critically involved in the pathway of apoptosis. We have conjugated a MTS-transport-peptide to monoclonal and polyclonal anti-caspase-3 antibodies to suppress Actinomycin D-induced apoptosis in human lymphoma T cells. The advantage of using trans-membrane antibodies compared to conventional apoptosis inhibitors is their specific target recognition in the living cell and their lower toxicity compared to conventional apoptosis inhibitors. We could show that a MTS-transport-peptide modified monoclonal anti-caspase-3 antibody reduces Actinomycin D induced apoptosis, as shown by DNA ladder electrophoresis and cell death ELISA. These results indicate that antibodies have a therapeutic potential to inhibit apoptosis in a variety of diseases.
Zhao
Y
Y
Immpheron Inc., Lexington, KY 40503, USA.
Brown
T L
TL
Kohler
H
H
Müller
S
S
D000906:Antibodies / Q000276:immunology / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D053148:Caspase 3; D020169:Caspases / Q000276:immunology*; D002455:Cell Division; D053938:DNA Fragmentation / Q000187:drug effects; D003609:Dactinomycin / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D013049:Spectrin / Q000187:drug effects
D000906:Antibodies; D013049:Spectrin; D003609:Dactinomycin; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases