Concatenation cDNA sequencing for transcriptome analysis.
We describe a high-throughput cDNA sequencing pipeline (http://www.hgsc.bcm.tmc.edu/projects/cdna) built in response to the emerging need for rapid sequencing of large cDNA collections. Using this strategy cDNA inserts are purified and joined through concatenation into large molecules. These 'pseudo-BACs' are subjected to random shotgun sequencing whereby the majority of cDNA inserts in the pool are sequenced. Using this concatenation cDNA sequencing platform, we have contributed more than 13000 full-length cDNA sequences from human and mouse to the Mammalian Gene Collection (MGC).
Gunaratne
Preethi H
PH
Human Genome Sequencing Center, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA. preethig@bcm.tmc.edu
Wu
Jia Qian
JQ
Garcia
Angela M
AM
Hulyk
Steven
S
Worley
Kim C
KC
Margolin
Judith F
JF
Gibbs
Richard A
RA
D003001:Cloning, Molecular; D018076:DNA, Complementary; D044708:DNA, Concatenated; D005821:Genetic Techniques; D006801:Humans; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
D018076:DNA, Complementary; D044708:DNA, Concatenated