Separation and purification of puerarin using beta-cyclodextrin-coupled agarose gel media.
The isoflavonoid puerarin, a well-known traditional Chinese drug, has been purified in one step from an extract of Radix puerariae (root of the plant Pueralria lobata) by adsorption chromatography on an epichlorohydrin polymerized beta-cyclodextrin ligand coupled to brominated allyl-group substituted Sepharose HP. Acetic acid (10%) was used as the mobile phase and the optimum loading capacity was around 1.2 mg crude extract/ml packed gel. The purity of the collected puerarin was about 98% with a recovery of about 62%.
He
Xiangling
X
Department of Bioengineering, Beijing University of Chemical Technology, Beijing 100029, China.
Tan
Tianwei
T
Xu
Bingze
B
Janson
Jan-Christer
JC
D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins / Q000737:chemistry*; D007529:Isoflavones / Q000302:isolation & purification*; D012685:Sepharose / Q000737:chemistry*; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D007529:Isoflavones; D047392:beta-Cyclodextrins; D012685:Sepharose; C031215:betadex; C033607:puerarin