Ebselen-mediated protection from single and repeated noise exposure in rat.
OBJECTIVE
Ebselen, a glutathione peroxidase mimic, is a candidate compound for the prevention of noise-induced hearing loss.


METHODS
Single-blinded, placebo-controlled study.


METHODS
Methods included single and repeated noise exposures on F-344 female rats given oral or injected ebselen or vehicle before and after noise, evoked auditory brainstem responses using click and pure-tone stimuli, light and fluorescence microscopy of cochleae stained with 4',6-Diamidino-2-phenylindole (DAPI) and fluorescein isothiocyanate-phalloidin, and statistical power determined by ANOVA.


RESULTS
Auditory brainstem response indicated that ebselen provided significant protection from both temporary and permanent threshold shifts following single and repeated noise exposure. On average, three times more outer hair cells were lost in control versus ebselen-treated animals.


CONCLUSIONS
Ebselen reduces noise-induced hearing loss in rats.
Lynch
Eric D
ED
Sound Pharmaceuticals, Inc, Seattle, WA 98103, USA. elynch@soundpharmaceuticals.com
Gu
Rende
R
Pierce
Carol
C
Kil
Jonathan
J
D000284:Administration, Oral; D000818:Animals; D001393:Azoles / Q000008:administration & dosage / Q000627:therapeutic use*; D003051:Cochlea / Q000166:cytology; D016057:Evoked Potentials, Auditory, Brain Stem; D005260:Female; D006198:Hair Cells, Auditory / Q000166:cytology; D006317:Hearing Loss, Noise-Induced / Q000517:prevention & control*; D054833:Isoindoles; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D016566:Organoselenium Compounds / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats; D011916:Rats, Inbred F344
D001393:Azoles; D054833:Isoindoles; D018696:Neuroprotective Agents; D016566:Organoselenium Compounds; C042986:ebselen