Biochemical characterization of symbiosome membrane proteins from Medicago truncatula root nodules.
The symbiosome membrane represents a specialized plant membrane that forms both a structural and a functional interface between the legume plant and its bacterial counterpart. In this study, the symbiosome membrane protein profile from the model system Medicago truncatula and the corresponding bacterium Sinorhizobium meliloti was examined using two-dimensional electrophoresis and microcapillary high-performance liquid chromatography (HPLC) tandem mass spectrometry. The identities of 51 proteins were obtained and these proteins were categorized into functional classes to indicate biochemical roles. Symbiosome membrane proteins include an H(+)-ATPase, ENOD16, ENOD8, nodulin-25, BiP, HSP70, PDI, multifunctional aquaporin, a putative syntaxin, and other proteins of known and unknown identity and function. The majority of the proteins identified were involved with protein destination and storage. These results allow us to understand better the biochemical composition of the symbiosome membrane and thus provide a basis to hypothesize mechanisms of symbiosome membrane formation and function.
Catalano
Christina M
CM
Department of Plant and Soil Sciences and the Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, USA.
Lane
William S
WS
Sherrier
D Janine
DJ
D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D007887:Fabaceae / Q000737:chemistry / Q000382:microbiology; D029902:Medicago / Q000737:chemistry* / Q000382:microbiology; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000502:physiology; D010940:Plant Proteins / Q000302:isolation & purification* / Q000502:physiology; D018517:Plant Roots / Q000737:chemistry / Q000382:microbiology; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D016962:Sinorhizobium meliloti; D013559:Symbiosis
D008565:Membrane Proteins; D010940:Plant Proteins