[Effect of T-cell vaccination in murine experimental autoimmune hepatitis].
OBJECTIVE
To investigate the effect of T-cell vaccination in murine experimental autoimmune hepatitis (EAH).


METHODS
To induce the EAH model, the syngeneic S-100 antigen emulsified in complete Freud's adjuvant was injected intraperitoneally to C57Bl/6 at day 1 and day 7. For T-cell vaccination, splenocytes were removed from animal 2 weeks after induction of EAH and from control animals, and activated in vitro by mitogen stimulation with Concanavalin A (Con A), then inactivated by mitomycin and injected at 5 10(7) cells per animal as T-cell vaccination at 14 and 7 days before first induction of EAH.


RESULTS
The histological grade and serum ALT level of the mice who received T-cell vaccination were decrease significantly, compared with that of model group (1.44+/-0.88 vs. 2.33+/-0.87, t=2.24, P<0.05; 63.0U/L+/-23.4U/L vs. 115.0U/L1+/-39.6U/L, t=2.37, P<0.01, respectively); there was no significant change in mice who received irrelevant T-cell vaccination.


CONCLUSIONS
T-cell vaccination with T cells from EAH animals, but not with irrelevant T cells, was able to protect animals from EAH.
Ma
Xiong
X
Shanghai institute of Digestive Disease, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China.
Qiu
De-kai
DK
Li
En-ling
EL
Peng
Yan-shen
YS
Chen
Xiao-yu
XY
D000818:Animals; D019693:Hepatitis, Autoimmune / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology*; D014611:Vaccination