title: Experimental diabetes accelerates accumulation of fluorescent pigments in rat trigeminal neurons.
abstract: The purpose of this study was to investigate autofluorescent pigment granules (lipofuscin, ceroid) in the trigeminal neurons (TN) during aging and streptozotocin-induced diabetes. Four young adult male rats were injected with streptozotocin (STZ; 50 mg/kg) to produce diabetes (DM), for comparison with four young uninjected control rats and four aged rats (90 weeks old). Eight weeks after STZ injection, all rats were fixed with 4% paraformaldehyde, and paraffin sections of TN were prepared and observed by fluorescence microscopy. We found the number of neurons with autofluorescent pigments had increased to 30.38% of the total in DM compared to 8.98% in the control group, and 25.36% in the aged rats. The area of autofluorescence within those neurons was 16.84% in aged rats, 13.02% in DM and 4.45% in the controls. Thus, DM caused accelerated accumulation of fluorescent granules in trigeminal neurons, and this change may show that premature aging contributes to neuronal functional decline and morphological change.
authors.affiliation: Department of Periodontology, Kanagawa Dental College, 82, Inaoka-cho, Yokosuka, 238-8580, Japan. sugayaak@kdcnet.ac.jp
authors.forename: Akira
authors.initials: A
authors.lastname: Sugaya
authors.forename: Hisashi
authors.initials: H
authors.lastname: Sugimioto
authors.forename: Nobumichi
authors.initials: N
authors.lastname: Mogi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsujigami
authors.forename: Shinji
authors.initials: S
authors.lastname: Deguchi
mesh_terms: D019588:Aging, Premature / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D000818:Animals; D002566:Ceroid / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D003929:Diabetic Neuropathies / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D004195:Disease Models, Animal; D005453:Fluorescence; D017127:Glycation End Products, Advanced / Q000378:metabolism; D008062:Lipofuscin / Q000378:metabolism*; D008297:Male; D009410:Nerve Degeneration / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009475:Neurons, Afferent / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D012668:Trigeminal Ganglion / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology
chemical_list: D002566:Ceroid; D017127:Glycation End Products, Advanced; D008062:Lipofuscin; D009419:Nerve Tissue Proteins