Isolation of functional RNA from small amounts of different grape and apple tissues.
An efficient, simple, and small-scale procedure for isolating functional ribonucleic acid (RNA) was successfully applied to many different tissues of grape and apple. These woody plants are rich in polyphenolic compounds and polysaccharides that could impair the RNA extraction. The method chosen is based on the use of hot borate buffer at alkaline pH supplemented with several adjuvants and followed by selective precipitations. Starting with only 0.4 g of fresh tissue and working with small tubes (2 mL), we were able to obtain good yields of high-quality RNA suitable for further applications. The procedure can be proposed for many applications, and it is particularly highly recommended when isolating RNA from a large number of samples.
Moser
Claudio
C
Istituto Agrario di S. Michele all'Adige, I-38010, S. Michele a/Adige, Trento-Italy. claudio.moser@mail.ismaa.it
Gatto
Pamela
P
Moser
Mirko
M
Pindo
Massimo
M
Velasco
Riccardo
R
D011232:Chemical Precipitation; D027845:Malus / Q000235:genetics*; D008832:Microchemistry / Q000379:methods*; D010936:Plant Extracts / Q000235:genetics* / Q000302:isolation & purification*; D018749:RNA, Plant / Q000302:isolation & purification*; D014461:Ultracentrifugation / Q000379:methods*; D027843:Vitis / Q000235:genetics*
D010936:Plant Extracts; D018749:RNA, Plant