title: Novel method for cell immobilization and its application for production of oligosaccharides from sucrose.
abstract: OBJECTIVE
  The purpose of the present investigation was to develop a novel method for cell immobilization.
  
  
  RESULTS
  Aureobasidium pullulans cells were mixed with an alginate solution, and the mixture was extruded to form small gel beads as hydrated-immobilized cells. The beads were then placed at -15 degrees C for 6-24 h to induce freeze-dehydration. The freeze-dehydration resulted in shrinkage of beads as a result of water removal reducing bead volume by 82% and bead weight by 85%. The dehydrated beads were successfully used for the production of fructo-oligosaccharides in a model reactor system.
  
  
  CONCLUSIONS
  Dehydrated beads may provide some commercial advantages over conventional immobilized cells.
  
  
  CONCLUSIONS
  This study shows that bioreactor performance can be improved up to two times by the use of the dehydrated beads.
authors.affiliation: Faculty of Life Science and Technology, Sungkyunkwan University, Suwon, Kyunggi-do, Korea.
authors.forename: H T
authors.initials: HT
authors.lastname: Shin
authors.forename: K M
authors.initials: KM
authors.lastname: Park
authors.forename: K H
authors.initials: KH
authors.lastname: Kang
authors.forename: D J
authors.initials: DJ
authors.lastname: Oh
authors.forename: S W
authors.initials: SW
authors.lastname: Lee
authors.forename: S Y
authors.initials: SY
authors.lastname: Baig
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000464:Alginates / Q000378:metabolism; D001203:Ascomycota / Q000166:cytology / Q000378:metabolism*; D019149:Bioreactors; D002448:Cell Adhesion; D018914:Cells, Immobilized; D003681:Dehydration / Q000382:microbiology; D004795:Enzyme Stability; D005285:Fermentation; D005612:Freeze Drying; D020723:Glucuronic Acid / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D008863:Microspheres; D009844:Oligosaccharides / Q000096:biosynthesis; D013395:Sucrose / Q000378:metabolism; D013696:Temperature
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D009844:Oligosaccharides; C116580:fructooligosaccharide; D013395:Sucrose; D020723:Glucuronic Acid