Sphingosine may have cytotoxic effects via apoptosis on the growth of keloid fibroblasts.
Keloids are often resistant to treatment, causing much suffering to the patient. Our previous work found that ceramide (Cer) inhibits growth of fibroblasts via apoptosis. However, when compared to normal fibroblasts (NFs), which are quiescent, keloid fibroblasts (KFs) rapidly proliferate and are reported to be resistant to apoptosis via Cer. Sphingosine (Sph) is a metabolite product of ceramide that has some different biochemical properties. Thereofore, we investigated the cytotoxic effects of Sph on cultured fibroblasts from keloid lesions and normal skin in order to evaluate the possibility of using Sph in the treatment of keloid. We used the lactic dehydrogenase (LDH) method, MTT method, and propidium iodide (PI) method. Sph had cytotoxic effects via apoptosis on both the KFs and NFs. Our results indicate that Sph may be applicable to the future treatment of keloid.
Chang
Sung-Eun
SE
Department of Dermatology, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea.
Kim
Kyoung-Jin
KJ
Ro
Kyoung-Hyun
KH
Lim
Young-Jin
YJ
Choi
Jee-Ho
JH
Moon
Kee-Chan
KC
Sung
Kyung-Jeh
KJ
D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D001707:Biopsy, Needle; D016022:Case-Control Studies; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival; D002478:Cells, Cultured; D002518:Ceramides / Q000378:metabolism / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007627:Keloid / Q000188:drug therapy / Q000473:pathology*; D012016:Reference Values; D012307:Risk Factors; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D013110:Sphingosine / Q000494:pharmacology / Q000633:toxicity*
D002518:Ceramides; D013110:Sphingosine