title: Decrease of mitochondrial DNA content and energy metabolism in renal cell carcinoma.
abstract: To elucidate the relationship between tumor genesis and the mitochondrial energy metabolism in renal neoplasms, we studied three individual enzyme activities of the oxidative phosphorylation, two components of the Krebs cycle and the mitochondrial DNA content of renal carcinomas including 29 conventional, five papillary, two unclassified carcinomas with sarcomatoid features and one collecting duct carcinoma. A significant reduction of all mitochondrial enzyme activities including complex V, as well as of the mitochondrial DNA content was detected in 34 of 37 renal carcinoma tissues as compared with control kidney. Mitochondrial enzyme activities and mitochondrial DNA levels were not statistically different between the conventional, papillary and unclassified sarcomatoid type of renal carcinoma and did not correlate with tumour grade, metastasis, ploidy and proliferative activity as determined by Ki-67 staining. Taken together, our data indicate that a co-ordinated down-regulation of all components necessary for mitochondrial energy metabolism occurs in most renal carcinomas as an early event in carcinoma formation, which does not change with progression of the disease.
authors.affiliation: Department of Pediatrics, Paracelsus Private Medical University Salzburg, Muellner Hauptstr. 48, A-5020 Salzburg, Austria.
authors.forename: David
authors.initials: D
authors.lastname: Meierhofer
authors.forename: Johannes A
authors.initials: JA
authors.lastname: Mayr
authors.forename: Ulrike
authors.initials: U
authors.lastname: Foetschl
authors.forename: Alexandra
authors.initials: A
authors.lastname: Berger
authors.forename: Klaus
authors.initials: K
authors.lastname: Fink
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Schmeller
authors.forename: Gerhard W
authors.initials: GW
authors.lastname: Hacker
authors.forename: Cornelia
authors.initials: C
authors.lastname: Hauser-Kronberger
authors.forename: Barbara
authors.initials: B
authors.lastname: Kofler
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Sperl
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D002292:Carcinoma, Renal Cell / Q000378:metabolism*; D004272:DNA, Mitochondrial / Q000378:metabolism*; D004734:Energy Metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
chemical_list: D004272:DNA, Mitochondrial