Effects of various culture environments on expression of major outer membrane proteins from Porphyromonas gingivalis.
We examined the effects of various culture environments on major outer membrane proteins from Porphyromonas gingivalis ATCC 33277. Major outer membrane protein patterns on gel electrophoresis showed little difference over the culturable range of osmolarity and pH. With elevated temperature or prolonged culture, the intensities of the gingipain bands decreased; however, bands of RagA, RagB and the putative porins were relatively stable. Similar results were observed with several different culture media. Although the precise functions of RagA, RagB and the putative porins are unknown, these factors may be strongly related to the initiation and progression of adult periodontitis.
Murakami
Yukitaka
Y
Department of Microbiology, School of Dentistry, Aichi-Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi 464-8650, Japan. yukimu@dpc.aichi-gakuin.ac.jp
Imai
Masashi
M
Mukai
Yuichi
Y
Ichihara
Shigeyuki
S
Nakamura
Hiroshi
H
Yoshimura
Fuminobu
F
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D001426:Bacterial Proteins; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D020559:Monomeric GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D009994:Osmolar Concentration; D016966:Porphyromonas gingivalis / Q000254:growth & development* / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D003470:Culture Media; C484343:RagA protein, Porphyromonas gingivalis; C484346:RagB protein, bacteria; D020559:Monomeric GTP-Binding Proteins