title: Effect of endodontic agents on cytotoxicity induction by sodium fluoride.
abstract: We investigated six endodontic agents for their ability to induce apoptosis and modify the cytotoxic activity of NaF against human squamous cell carcinoma (HSC-2) and human promyelocytic leukemia (HL-60) cell lines. Four Group I agents (Form Cresol, Cam Phenic, Eucaly Soft, GC Fuji Varnish), but not two Group II agents (Caviton, Canals-N), induced internucleosomal DNA fragmentation and activated caspases 3, 8 and 9 in HL-60 cells. Only Cam Phenic among these agents additively enhanced the cytotoxic activity of NaF in HSC-2 and HL-60 cells. Form Cresol and Cam Phenic reduced the glucose consumption at early stage, possibly due to their toxic effect. Amino acid analysis suggests that the higher cytotoxicity of Form Cresol may be derived, at least in part, from its oxidizing action.
authors.affiliation: Department of Dental Pharmacology, Meikai University School of Dentistry, Sakado, Saitama, 350-0283, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Tokunaga
authors.forename: Sufi Reza Md
authors.initials: SR
authors.lastname: Morshed
authors.forename: Sumiko
authors.initials: S
authors.lastname: Otsuki
authors.forename: Fumitoshi
authors.initials: F
authors.lastname: Takayama
authors.forename: Ken
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Masanori
authors.initials: M
authors.lastname: Kashimata
authors.forename: Yuko
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Nishikawa
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Yasui
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Yokote
authors.forename: Kiso
authors.initials: K
authors.lastname: Akahane
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakagami
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002133:Calcium Sulfate / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9; D020169:Caspases / Q000378:metabolism; D003738:Dental Cements; D004338:Drug Combinations; D005947:Glucose / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute; D008715:Methionine / Q000378:metabolism; D009062:Mouth Neoplasms; D009910:Oral Hygiene; D010084:Oxidation-Reduction; D012387:Root Canal Filling Materials; D012969:Sodium Fluoride / Q000494:pharmacology*; D014753:Vinyl Compounds / Q000494:pharmacology; D015034:Zinc Oxide / Q000494:pharmacology
chemical_list: D003738:Dental Cements; D004338:Drug Combinations; D012387:Root Canal Filling Materials; D014753:Vinyl Compounds; C086076:calcium sulfate, zinc oxide, vinyl acetate, zinc phosphate root canal filling; D012969:Sodium Fluoride; D008715:Methionine; C505407:CASP3 protein, human; C505530:CASP8 protein, human; C506433:CASP9 protein, human; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9; D020169:Caspases; D005947:Glucose; D015034:Zinc Oxide; D002133:Calcium Sulfate