Capillary isoelectric focusing in pseudo-closed channel coupled to matrix assisted laser desorption/ionization mass spectrometry for protein analysis.
Capillary isoelectric focusing (CIEF) was performed in pseudo-closed channel to separate proteins on a plastic chip. Pseudo-closed channel provided a novel way to couple protein separation by CIEF to MALDI mass spectrometry without eluting the focused proteins.
Mok
Michelle L-S
ML
Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672, Singapore. szen@gis.a-star.edu.sg
Hua
Lin
L
Phua
Jonathan B-C
JB
Wee
Michael K-T
MK
Sze
Newman S-K
NS
D000818:Animals; D002417:Cattle; D006736:Horses; D007525:Isoelectric Focusing / Q000379:methods; D009211:Myoglobin / Q000032:analysis; D040081:Protein Array Analysis / Q000379:methods; D011506:Proteins / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D009211:Myoglobin; D011506:Proteins