Molecular architecture of photosynthetic membranes in Rhodobacter sphaeroides: the role of PufX.
The effects of the PufX polypeptide on membrane architecture were investigated by comparing the composition and structures of photosynthetic membranes from PufX+ and PufX- strains of Rhodobacter sphaeroides. We show that this single polypeptide profoundly affects membrane morphology, leading to highly elongated cells containing extended tubular membranes. Purified tubular membranes contain helical arrays composed solely of dimeric RC-LH1-PufX (RC, reaction centre; LH, light harvesting) complexes with apparently open LH1 rings. PufX- cells contain crystalline membranes with a pseudo-hexagonal packing of monomeric core complexes. Analysis of purified complexes by electron microscopy and atomic force microscopy shows that LH1 and PufX form a continuous ring of protein around each RC. A model of the tubular membrane is presented with PufX located adjacent to the stained region created by a vacant LH1beta. This arrangement, coupled with a flexible ring, would give the RC QB site transient access to the interstices in the lattice, which might be of functional importance. We discuss the implications of our data for the export of quinol from the RC, for eventual reduction of the cytochrome bc1 complex.
Siebert
C Alistair
CA
Department of Molecular Biology and Biotechnology, University of Sheffield, Krebs Institute for Biomolecular Research, Sheffield, UK.
Qian
Pu
P
Fotiadis
Dimitrios
D
Engel
Andreas
A
Hunter
C Neil
CN
Bullough
Per A
PA
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure*; D002499:Centrifugation, Density Gradient; D003461:Crystallography; D019281:Dimerization; D014450:Electron Transport Complex III / Q000235:genetics / Q000378:metabolism; D005583:Fourier Analysis; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D008854:Microscopy, Electron; D009154:Mutation; D012242:Rhodobacter sphaeroides / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure
D001426:Bacterial Proteins; D045342:Light-Harvesting Protein Complexes; C088749:PufX protein, Rhodobacter; D014450:Electron Transport Complex III