title: Prolonged outbreak of human parainfluenza virus 3 infection in a stem cell transplant outpatient department: insights from molecular epidemiologic analysis.
abstract: Human parainfluenza virus type 3 (hPIV3) infections cause considerable morbidity and mortality after stem cell transplantation, and inpatient nosocomial outbreaks are common. From September 1998 to July 1999, 93 stem cell transplantation recipients at our institution contracted hPIV3, of which 66 (71%) were being followed up in our outpatient department (OPD). The peak incidence was in September and October, when 39 cases were identified; thereafter, hPIV3 incidence decreased to approximately 5 cases per month. Nucleotide sequences (778 nucleotides from variable regions of the hemagglutinin-neuraminidase gene) from 46 patient and 8 community hPIV3 isolates were compared to determine epidemiologic relatedness. Sequence analysis of OPD isolates revealed that 18 of 19 isolates from September and October and 11 of 15 isolates from November 1998 to July 1999 were genetically similar. In contrast, 2 of 3 community isolates from September and October and 0 of 5 from November to July were linked to this cluster. Symptomatic surveillance and isolation were ineffective in terminating the outbreak, suggesting asymptomatic shedding among patients, staff, or visitors or viral persistence on environmental surfaces as possible explanations. The concept of nosocomial transmission should be expanded to include the OPD for immunosuppressed patients.
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-4417, USA. gnichols@fhcrc.org
authors.forename: W Garrett
authors.initials: WG
authors.lastname: Nichols
authors.forename: Dean D
authors.initials: DD
authors.lastname: Erdman
authors.forename: Alison
authors.initials: A
authors.lastname: Han
authors.forename: Carol
authors.initials: C
authors.lastname: Zukerman
authors.forename: Lawrence
authors.initials: L
authors.lastname: Corey
authors.forename: Michael
authors.initials: M
authors.lastname: Boeckh
mesh_terms: D003428:Cross Infection; D004196:Disease Outbreaks; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006748:Hospital Departments; D006801:Humans; D016867:Immunocompromised Host; D015994:Incidence; D017720:Molecular Epidemiology; D010224:Parainfluenza Virus 3, Human / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis; D010253:Respirovirus Infections / Q000453:epidemiology* / Q000209:etiology / Q000635:transmission; D017423:Sequence Analysis, RNA; D014861:Washington / Q000453:epidemiology
chemical_list: D012367:RNA, Viral