title: Direct vs. indirect effects of trypan blue on the ectodermal cells of the 11.5-day rat embryo?
abstract: The effect of trypan blue on the 11.5-day rat conceptus after intravitelline vessel administration is described. For comparison, conceptuses injected with varying volumes of Hanks' BSS have also been studied. Trypan blue significantly retarded the growth and development of conceptuses after 6 hours incubation in vitro. The SEM revealed rounded ectodermal cells, some of which appeared disrupted. These cells seemed to cause some of the intersomitic grooves to disappear, making a number of the somites indistinct from the outside. Unlike cells of uninjected embryos, the surfaces of the affected ectodermal cells lacked microvilli and their perimeters were lined with microvilli-like structures which appeared matted together. It was concluded that trypan blue affected the embryo directly probably by disturbing its fluid and ionic balance.
authors.affiliation: Department of Anatomy, University of Leicester, UK.
authors.forename: E P
authors.initials: EP
authors.lastname: Mensah-Brown
mesh_terms: D000818:Animals; D004475:Ectoderm / Q000166:cytology* / Q000187:drug effects*; D005260:Female; D005317:Fetal Growth Retardation / Q000139:chemically induced; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000187:drug effects / Q000648:ultrastructure; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D013723:Teratogens / Q000494:pharmacology*; D014343:Trypan Blue / Q000494:pharmacology*; D015017:Yolk Sac / Q000166:cytology / Q000187:drug effects
chemical_list: D013723:Teratogens; D014343:Trypan Blue