[Purification and physi-chemical properties of polysaccharides SJZPS-Vb-1-2 from immunocompetence parts of sijunzi decoction].
OBJECTIVE
To supply basic research materials for clarifying the immunocompetence materials of Sijunzi Decoction.


METHODS
Two polysaccharides(SJZPS-Vb-1-2) were purified with Sephadex DEAE A 25 and Sephadex G 200 from SJZPS-Vb part (with the highest immunocompetence). Their molecular weights were determined. The sugar compositions were analyzed with GC. The linkage positions of the component sugars were determined by methylation and GC/MS.


RESULTS
SJZPS-Vb-1-2 was uonic-containing heteropolysaccharides with the component sugars of glucose, galactose and mannose in different molar ratios. The molecular weight of SJZPS-VB-1 was 38,300, SJZPS-VB-2, 26,000. The molar ratio of SJZPS-Vb-1 was as glu:gal:man 1:046:2.15 and SJZPS-Vb-2, glu:gal:man 1:1.41:4.18.
Zhu
Chenchen
C
Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405.
Gui
Shuhua
S
Wang
Peixun
P
Chen
Weiwen
W
D000818:Animals; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D005260:Female; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006460:Hemolysin Proteins / Q000276:immunology*; D007121:Immunocompetence / Q000276:immunology; D008297:Male; D008358:Mannose / Q000032:analysis; D051379:Mice; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D006460:Hemolysin Proteins; D009005:Monosaccharides; D011134:Polysaccharides; D005947:Glucose; D008358:Mannose; D005690:Galactose