title: Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates.
abstract: DNA from over 300 Bacillus thuringiensis, Bacillus cereus, and Bacillus anthracis isolates was analyzed by fluorescent amplified fragment length polymorphism (AFLP). B. thuringiensis and B. cereus isolates were from diverse sources and locations, including soil, clinical isolates and food products causing diarrheal and emetic outbreaks, and type strains from the American Type Culture Collection, and over 200 B. thuringiensis isolates representing 36 serovars or subspecies were from the U.S. Department of Agriculture collection. Twenty-four diverse B. anthracis isolates were also included. Phylogenetic analysis of AFLP data revealed extensive diversity within B. thuringiensis and B. cereus compared to the monomorphic nature of B. anthracis. All of the B. anthracis strains were more closely related to each other than to any other Bacillus isolate, while B. cereus and B. thuringiensis strains populated the entire tree. Ten distinct branches were defined, with many branches containing both B. cereus and B. thuringiensis isolates. A single branch contained all the B. anthracis isolates plus an unusual B. thuringiensis isolate that is pathogenic in mice. In contrast, B. thuringiensis subsp. kurstaki (ATCC 33679) and other isolates used to prepare insecticides mapped distal to the B. anthracis isolates. The interspersion of B. cereus and B. thuringiensis isolates within the phylogenetic tree suggests that phenotypic traits used to distinguish between these two species do not reflect the genomic content of the different isolates and that horizontal gene transfer plays an important role in establishing the phenotype of each of these microbes. B. thuringiensis isolates of a particular subspecies tended to cluster together.
authors.affiliation: Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
authors.forename: Karen K
authors.initials: KK
authors.lastname: Hill
authors.forename: Lawrence O
authors.initials: LO
authors.lastname: Ticknor
authors.forename: Richard T
authors.initials: RT
authors.lastname: Okinaka
authors.forename: Michelle
authors.initials: M
authors.lastname: Asay
authors.forename: Heather
authors.initials: H
authors.lastname: Blair
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Bliss
authors.forename: Mariam
authors.initials: M
authors.lastname: Laker
authors.forename: Paige E
authors.initials: PE
authors.lastname: Pardington
authors.forename: Amber P
authors.initials: AP
authors.lastname: Richardson
authors.forename: Melinda
authors.initials: M
authors.lastname: Tonks
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Beecher
authors.forename: John D
authors.initials: JD
authors.lastname: Kemp
authors.forename: Anne-Brit
authors.initials: AB
authors.lastname: Kolstø
authors.forename: Amy C Lee
authors.initials: AC
authors.lastname: Wong
authors.forename: Paul
authors.initials: P
authors.lastname: Keim
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Jackson
mesh_terms: D000818:Animals; D001408:Bacillus anthracis / Q000145:classification* / Q000235:genetics; D001409:Bacillus cereus / Q000145:classification* / Q000235:genetics; D001413:Bacillus thuringiensis / Q000145:classification* / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005453:Fluorescence; D006801:Humans; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping
chemical_list: D004269:DNA, Bacterial; D015246:Deoxyribonuclease EcoRI; C055927:endodeoxyribonuclease MseI; D015252:Deoxyribonucleases, Type II Site-Specific