Extracellular chitinase production by Trichoderma harzianum in submerged fermentation.
Extra-cellular chitinase production by a chitinolytic fungus Trichoderma harzianum TUBF 966 using submerged fermentation was studied. Colloidal chitin (1.5% w/v) was used as sole carbon source. Maximum chitinase production (14.7 U/ml) was obtained when fermentation was carried out at 30 degrees C for 96 h using 72 h old mycelium in a medium containing colloidal chitin 1.5% (w/v) as carbon source and 0.42 (% w/v) peptone as nitrogen source (pH 5.5). Supplementation of additional carbon sources (0.75% w/v) showed no further enhancement in chitinase production while supplementation of nitrogen sources (0.42% w/v) such as peptone and tryptone in the fermentation medium showed a marked increase in production. The process parameters that controlled chitinase production by the fungus were studied and presented here.
Sandhya
C
C
Biotechnology Division, Regional Research Laboratory, CSIR, Trivandrum-695 019, India.
Adapa
Leela Krishna
LK
Nampoothiri
K Madhavan
KM
Binod
P
P
Szakacs
George
G
Pandey
Ashok
A
D002244:Carbon / Q000378:metabolism; D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000096:biosynthesis* / Q000378:metabolism; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D025282:Mycelium / Q000254:growth & development / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D010461:Peptones / Q000378:metabolism; D013172:Spores, Fungal / Q000254:growth & development; D013696:Temperature; D013997:Time Factors; D014242:Trichoderma / Q000201:enzymology* / Q000254:growth & development* / Q000378:metabolism
D010461:Peptones; D002686:Chitin; C001830:tryptones; D002244:Carbon; D002688:Chitinases; D009584:Nitrogen