title: [Application of sleeve anastomosis in development of cervical heart transplantation in rats].
abstract: OBJECTIVE
  To simplify surgical technique and increase postoperative survival rate, sleeve anastomosis technique combined cuff technique was used in developing the model of cervical heart transplantation in rats.
  
  
  METHODS
  In this model, the hearts from 25 male SD rats were transplanted into the neck of Wistar rats by anastomosing the donor innominate artery to the recipient right common carotid artery by use of sleeve technique, and the donor pulmonary artery to the recipient right external jugular vein by use of cuff technique. After operation, the rats were treated with cyclosporine A (1.5 mg/kg, q.d.), transplanted hearts were followed by daily inspection or palpation and the allograft survival time was more than 3 days as the standard of successful operation.
  
  
  RESULTS
  The mean operative time was (48.7 +/- 3.4) min, with a successful rate of 88% (22/25). Complications were anastomotic hemorrhage (1 case) and thrombosis(2 cases). During the follow-up period, 6 rats died of pulmonary infection, abscess in the neck, liver or bladder tumor. The remaining 16 transplanted hearts survived more than 3 months.
  
  
  CONCLUSIONS
  The modified operation have advantages of less operative procedure, shorter operation and ischemia time and easier monitoring of graft function.
authors.affiliation: Department of Surgery, Medical College, Shantou University, Shantou, Guangdong, P. R. China 515041.
authors.forename: Zhong-min
authors.initials: ZM
authors.lastname: Liu
authors.forename: Zhao-jie
authors.initials: ZJ
authors.lastname: Guan
authors.forename: Wen-lv
authors.initials: WL
authors.lastname: Shen
mesh_terms: D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D017536:Carotid Artery, Common / Q000601:surgery; D004195:Disease Models, Animal; D006085:Graft Survival; D016027:Heart Transplantation / Q000379:methods*; D007601:Jugular Veins / Q000601:surgery; D008297:Male; D009333:Neck; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013536:Suture Techniques; D016042:Transplantation, Heterotopic / Q000379:methods*; D014656:Vascular Surgical Procedures