Combination of alpha-fetoprotein mRNA-based detection of hematogenously disseminating hepatocellular carcinoma cells and analysis of cancer cell membrane fluidity is more accurate in screening patients at risk of postoperative recurrence.
We developed an mRNA-based, highly specific and sensitive method to detect hepatocellular carcinoma cells present in blood. However, the reason for some patients being positive for blood analysis and negative for recurrence has yet to be found. We recently established a method to measure membrane fluidity of hepatocellular carcinoma cells, and used it to analyze the actual membrane fluidity of human hepatocellular carcinoma cells. We found that patients with carcinoma cells with lower membrane fluidity less frequently developed recurrence. The analysis of both membrane fluidity and alpha-fetoprotein mRNA thus greatly increased the accuracy of the prediction of postoperative recurrence.
Funaki
Naomi
N
Department of Surgery, Shiga Medical Center for Adults, Moriyama City, Shiga 524-8524, Japan.
Tanaka
Junji
J
Kono
Yukihiro
Y
Nonaka
Atsushi
A
Yotsumoto
Fumiaki
F
Lee
Jeon-Uk
JU
Imamura
Masayuki
M
D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000401:mortality; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D018076:DNA, Complementary / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000378:metabolism* / Q000401:mortality; D008856:Microscopy, Fluorescence; D016133:Polymerase Chain Reaction; D011379:Prognosis; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012008:Recurrence; D016896:Treatment Outcome; D000509:alpha-Fetoproteins / Q000378:metabolism*
D018076:DNA, Complementary; D012333:RNA, Messenger; D000509:alpha-Fetoproteins; D012313:RNA