title: Isolation and characterization of a novel single-stranded RNA virus infecting the bloom-forming diatom Rhizosolenia setigera.
abstract: A novel single-stranded RNA (ssRNA) virus specifically infecting the bloom-forming diatom Rhizosolenia setigera (R. setigera RNA virus [RsRNAV]) was isolated from Ariake Sea, Japan. Viral replication occurred within the cytoplasm, and the virus particle was icosahedral, lacked a tail, and was 32 nm in diameter on average. The major nucleic acid extracted from the RsRNAV particles was an ssRNA molecule 11.2 kb in length, although smaller RNA molecules (0.6, 1.2, and 1.5 kb) were occasionally observed. The major structural proteins of RsRNAV were 41.5, 41.0, and 29.5 kDa. Inter- and intraspecies host specificity tests revealed that RsRNAV is not only species specific but also strain specific and that its intraspecies host specificity is diverse among virus clones. The latent period of RsRNAV was 2 days, and the burst sizes were 3,100 and 1,010 viruses per host cell when viruses were inoculated into the host culture at the exponential and stationary growth phases, respectively, at 15 degrees C under a 12-h-12-h light-dark cycle of ca. 110 micro mol of photons m(-2) s(-1) with cool white fluorescent illumination. To our knowledge, this is the first report describing the biological properties of a virus infecting a diatom. Further studies on RsRNAV will be helpful in understanding the ecological relationship between diatoms and viruses in nature.
authors.affiliation: National Research Institute of Fisheries and Environment of Inland Sea, Hiroshima 739-0452. Japan. nagasaki@affrc.go.jp
authors.forename: Keizo
authors.initials: K
authors.lastname: Nagasaki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Tomaru
authors.forename: Noriaki
authors.initials: N
authors.lastname: Katanozaka
authors.forename: Yoko
authors.initials: Y
authors.lastname: Shirai
authors.forename: Kensho
authors.initials: K
authors.lastname: Nishida
authors.forename: Shigeru
authors.initials: S
authors.lastname: Itakura
authors.forename: Mineo
authors.initials: M
authors.lastname: Yamaguchi
mesh_terms: D000818:Animals; D017377:Diatoms / Q000821:virology*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D012328:RNA Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D012623:Seawater; D013045:Species Specificity; D014779:Virus Replication
chemical_list: D012367:RNA, Viral