title: Age-associated increase in oxidative stress and nuclear factor kappaB activation are attenuated in rat liver by regular exercise.
abstract: The combined effects of aging and regular physical exercise was investigated on the production of reactive oxygen species (ROS), lipid peroxidation, glutathione status, and the activity of nuclear factor-kappaB (NF-kappaB) in rat liver. A group of 24 male F344 rats was divided into the following categories: adult control (18 months), adult exercised (18 months), and aged control (28 months) and aged exercised (28 months). The ROS formation increased as a function of age and exercise training decreased the rate of ROS formation in the two age groups. Significant positive correlation was found between ROS production and lipid peroxidation (LIPOX). The reduced glutathione (GSH) level was higher and the oxidized glutathione (GSSG) level lower in exercised groups compared with the sedentary controls (P<0.05). An age-associated increase in NF-kappaB activity was attenuated by the regular exercise. The content of p50 and p65 subunits of NF-kappaB increased with age and decreased with exercise training. The content of inhibitory factor-kappaB was inversely related to NF-kappaB activation. Regular exercise-induced adaptive responses, including attenuation of an increase in ROS production, LIPOX level, NF-kappaB activation, and reduced GSH/GSSG ratio, appear to be capable, even in old age, of reducing increases in inflammatory and other detrimental consequences that are often associated with advancing age.
authors.affiliation: Laboratory of Exercise Physiology, School of Sport Science, Semmelweis University, Budapest, Hungary. radak@mail.hupe.hu
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Radák
authors.forename: Hae Young
authors.initials: HY
authors.lastname: Chung
authors.forename: Hisashi
authors.initials: H
authors.lastname: Naito
authors.forename: Ryoya
authors.initials: R
authors.lastname: Takahashi
authors.forename: Kyung Jin
authors.initials: KJ
authors.lastname: Jung
authors.forename: Hyon-Jeen
authors.initials: HJ
authors.lastname: Kim
authors.forename: Sataro
authors.initials: S
authors.lastname: Goto
mesh_terms: D000375:Aging; D000818:Animals; D008099:Liver / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010805:Physical Conditioning, Animal; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D016328:NF-kappa B; D017382:Reactive Oxygen Species