Reduction of genotoxic effects of the carcinogen N-methyl-N'-nitro-N-nitrosoguanidine by dietary lignin in mammalian cells cultured in vitro.
In the present study the protective effect of several lignin polymers against the genotoxic effect of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) was tested in hamster lung V79 cells and human colon Caco-2 cells. Preculturing of cells with sublethal, nongenotoxic concentrations of the lignins A, B, and C (50 microg/ml) was found to decrease significantly the level of DNA strand breaks in both hamster and human cells treated with MNNG. Lignin A also reduced MNNG-induced gene mutations in V79 cells. As in addition to alkyl lesions MNNG gives rise to hydroxyl free radicals (OH) and nitrogen-centered free radicals (NR), we tried to determine whether antimutagenicity of lignin A was connected only with the well-known ability of lignin to bind MNNG molecules or also with its antioxidative effects. The use of the modified comet assay technique proved that preculturing of hamster V79 cells with lignin A resulted in a significant decrease of the level of DNA strand breaks originating from oxidized DNA bases. Therefore, we suggest that the antimutagenic effect of lignin A against MNNG is associated with both adsorptive and antioxidative action. This study also showed that the presence of lignin A neither helped to renew DNA replication nor influenced the kinetics of DNA rejoining in MNNG-treated V79 cells.
Lábaj
Juraj
J
Laboratory of Mutagenesis and Carcinogenesis, Cancer Research Institute, Slovak Academy of Sciences, Vlárska 7, 833 91 Bratislava, Slovak Republic. juraj21@yahoo.com
Slamenová
Darina
D
Kosikova
Bozena
B
D000818:Animals; D018938:Caco-2 Cells; D002273:Carcinogens / Q000633:toxicity*; D002460:Cell Line; D006224:Cricetinae; D004247:DNA / Q000096:biosynthesis; D004249:DNA Damage / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects; D004032:Diet; D006801:Humans; D008031:Lignin / Q000008:administration & dosage*; D008168:Lung; D008769:Methylnitronitrosoguanidine / Q000633:toxicity*; D009154:Mutation / Q000187:drug effects; D018384:Oxidative Stress
D002273:Carcinogens; D008769:Methylnitronitrosoguanidine; D008031:Lignin; D004247:DNA