Evaluation of genital sites and sampling techniques for detection of human papillomavirus DNA in men.
To evaluate methods for detection of genital human papillomavirus (HPV) DNA in men, samples were obtained from 3 consecutive groups of 10 men attending a sexually transmitted disease clinic by use of (1) a saline-wetted Dacron swab alone, (2) a saline-wetted cytobrush, or (3) emery paper (600A-grit Wetordry Tri-M-ite; 3M) abrasion followed by a saline-wetted Dacron swab. By use of a polymerase chain reaction-based assay, 45% of emery-paper samples were found to be positive for beta-globin, compared with 23% of swab-alone and 0% of cytobrush samples. Subsequently, emery paper and saline-wetted Dacron swabs were used to obtain penile shaft, glans, foreskin, and scrotum samples from 318 male university students. Urine samples were also obtained. Of 1323 samples tested, 1288 (97%) were found to be positive for beta-globin. HPV DNA was detected in samples from 104 men (33%): 24% from the penile shaft, 16% from the glans, 28% from the foreskin, 17% from the scrotum, and 6% in urine. The HPV prevalence was similar for circumcised and uncircumcised men. Testing multiple sites increased the number of men for whom HPV DNA was detected.
Weaver
Bethany A
BA
Center for AIDS & STD Research, Department of Medicine, University of Washington, Harborview Medical Center, 325 9th Avenue, Box 359931, Seattle, WA 98104 USA. bethanyg@u.washington.edu
Feng
Qinghua
Q
Holmes
King K
KK
Kiviat
Nancy
N
Lee
Shu-Kuang
SK
Meyer
Christine
C
Stern
Mike
M
Koutsky
Laura A
LA
D000293:Adolescent; D000328:Adult; D002944:Circumcision, Male; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D008297:Male; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D010413:Penis / Q000821:virology; D012611:Scrotum / Q000821:virology; D012725:Sexual Behavior; D013048:Specimen Handling / Q000379:methods; D014412:Tumor Virus Infections / Q000175:diagnosis*; D014556:Urine / Q000821:virology
D004279:DNA, Viral