Biosynthesis of tannase and gallic acid from tannin rich substrates by Rhizopus oryzae and Aspergillus foetidus.
Modified solid-state fermentation (MSSF) of tannin-rich substrates for production of tannase and gallic acid was carried out using two fungal cultures, Rhizopus oryzae (RO IIT RB-13, NRRL 21498) and Aspergillus foetidus (GMRB013 MTCC 3557). The tannin rich substrates included powdered fruits of Terminalia chebula and Caesalpinia digyna pod cover powder. The different environmental parameters for the maximum production of tannase and gallic acid were optimized through media engineering. The highest yield of tannase and gallic acid was obtained after 60 h in case of Rhizopus oryzae and after 72 h by Aspergillus foetidus with 3 ml of induced inoculum. The optimum initial pH of the fermentation was found to be 4.5 in case of Rhizopus oryzae and 5.0 for Aspergillus foetidus. MSSF was carried out at the optimum conditions of 30 degrees C and 80% relative humidity. Collectively, the data reveal the potential of the modified solid-state fermentation process for the production of tannase and gallic acid from tannin-rich substrates with R. oryzae and A. foetidus.
Mukherjee
Gargi
G
Microbial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur-721302, West Bengal, India.
Banerjee
Rintu
R
D001230:Aspergillus / Q000254:growth & development / Q000378:metabolism*; D019149:Bioreactors; D029911:Caesalpinia / Q000737:chemistry / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis*; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D005638:Fruit / Q000737:chemistry / Q000378:metabolism; D005656:Fungal Proteins / Q000096:biosynthesis; D005707:Gallic Acid / Q000378:metabolism*; D006813:Humidity; D006863:Hydrogen-Ion Concentration; D047348:Hydrolyzable Tannins / Q000378:metabolism*; D007218:Industrial Microbiology; D012233:Rhizopus / Q000254:growth & development / Q000378:metabolism*; D013696:Temperature; D029766:Terminalia / Q000737:chemistry / Q000378:metabolism; D013997:Time Factors
D003470:Culture Media; D005656:Fungal Proteins; D047348:Hydrolyzable Tannins; D005707:Gallic Acid; D002265:Carboxylic Ester Hydrolases; C022711:tannase