Gamma interferon-based immunodiagnosis of tuberculosis: comparison between whole-blood and enzyme-linked immunospot methods.
The gamma interferon secretion levels of 52 adults whose T cells had been stimulated with the Mycobacterium tuberculosis antigens CFP-10 and ESAT-6 were measured by enzyme-linked immunospot methods and a whole-blood-based assay. The test results correlated well (r = 0.689, P < 0.0001). Its simple format and use of only small volumes of blood make the whole-blood assay suitable for pediatric practice and field trials.
Schölvinck
Elisabeth
E
Department of Pediatrics. Wellcome Centre for Research in Clinical Tropical Medicine, Faculty of Medicine, Imperial College London, London W2 1PG.
Wilkinson
Katalin A
KA
Whelan
Adam O
AO
Martineau
Adrian R
AR
Levin
Michael
M
Wilkinson
Robert J
RJ
D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007371:Interferon-gamma / Q000097:blood*; D008213:Lymphocyte Activation; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014376:Tuberculosis / Q000175:diagnosis* / Q000276:immunology*
D000942:Antigens, Bacterial; D007371:Interferon-gamma