title: [Usefulness of arbitrarily primed polymerase chain reaction (AP-PCR) method for DNA fingerprinting analysis of Mycobacterium tuberculosis (MTB); comparison between DNA restriction fragment-length polymorphism (RFLP) method and AP-PCR method].
abstract: We have been analyzing cases suspected as outbreak of Mycobacterium tuberculosis in Tokyo using RFLP method. This time we analyzed 27 strains of MTB from 5 cases in two hospitals, a family, member of social activity and stuff of a corporation using both RFLP and AP-PCR methods. At 4 cases, over 80% of strains were same pattern in each cases with RFLP and AP-PCR and were identified as a patient to patients transmission of MTB. At one case, in a hospital, each strains were completely different patterns at both methods, which showed it was not a outbreak case. Results of RFLP and AP-PCR were completely same, which indicates AP-PCR is also useful and rapid method for epidemiological analysis of MTB infection as well as RFLP.
authors.affiliation: Department of Microbiology, Tokyo Metropolitan Institute of Public Health.
authors.forename: Jun
authors.initials: J
authors.lastname: Mukaigawa
authors.forename: Yukako
authors.initials: Y
authors.lastname: Shimojima
authors.forename: Iwao
authors.initials: I
authors.lastname: Murata
authors.forename: Miyoko
authors.initials: M
authors.lastname: Endou
authors.forename: Yositoki
authors.initials: Y
authors.lastname: Yanagawa
authors.forename: Satosi
authors.initials: S
authors.lastname: Morozumi
mesh_terms: D016358:Contact Tracing / Q000379:methods; D016172:DNA Fingerprinting / Q000379:methods*; D004196:Disease Outbreaks; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length