title: Apoptosis of auditory neurons following central process injury.
abstract: Although apoptotic changes in auditory neurons induced by injury to peripheral processes (dendrites) have been intensively studied, apoptotic changes in auditory neurons induced by injury to central processes (axons of spiral ganglion cells, SGCs) have not been reported previously, probably due to lack of an experimental model. The present study reports for the first time the appearance, extent, and time course of SGC apoptosis following injury to the central processes. Apoptosis was studied in a rat model that consisted of compression of the auditory nerve in the cerebellopontine (CP) angle cistern with intraoperative recordings of auditory nerve compound action potentials (CAPs) to ensure highly reproducible results. Rats were killed between day 0 and day 14 after compression and apoptosis of SGCs was evaluated quantitatively as well as qualitatively by terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) staining, anti-activated caspase-3 immunostaining, Hoechst 33342 staining, and electron microscopy. The average number of TUNEL-positive apoptotic SGCs in each cochlear turn increased from day 1 to day 5 and then decreased gradually to an undetectable level on day 14 after compression. The average proportion of apoptotic SGCs identified in any cochlear turn on any day was always lower than 10%. The results of our present study should be useful in determining the therapeutic time window for rescuing auditory neurons undergoing apoptosis due to injury during surgery in the CP angle.
authors.affiliation: Neurosurgery Department, Hirosaki University School of Medicine, Hirosaki 036-8216, Japan. seky@cc.hirosaki-u.ac.jp
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Sekiya
authors.forename: Akinori
authors.initials: A
authors.lastname: Yagihashi
authors.forename: Norihito
authors.initials: N
authors.lastname: Shimamura
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Asano
authors.forename: Shigeharu
authors.initials: S
authors.lastname: Suzuki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Matsubara
authors.forename: Atsushi
authors.initials: A
authors.lastname: Namba
authors.forename: Hideichi
authors.initials: H
authors.lastname: Shinkawa
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002530:Cerebellopontine Angle / Q000502:physiology; D003056:Cochlear Nerve / Q000473:pathology* / Q000502:physiology; D005072:Evoked Potentials, Auditory; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008297:Male; D008854:Microscopy, Electron; D009409:Nerve Crush; D051381:Rats; D017207:Rats, Sprague-Dawley; D013136:Spiral Ganglion / Q000473:pathology* / Q000502:physiology / Q000648:ultrastructure; D013997:Time Factors
chemical_list: C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases