[Effects of yi-fu-ning soft capsule on sex hormone, NO, NOS, SOD and MDA levels in ovariectomized rat serum].
OBJECTIVE
Through investigating the changs of sex hormone, nitric oxide (NO), nitric oxide synthase (NOS), superoxide dismutase (SOD) and malonaldehyde (MDA) in the serum of ovariectomized, rats, the mechanism of cardiovascular disease occurring in post-menopause rats and the effect of Yi-Fu-Ning soft capsule (YFN) on above-menticned indexes were discussed.


METHODS
Fifty female mature Sprague-Dawley rats were randomly divided into 5 groups: sham operation, ovariectomy (OVX), OVX with diethylstilbestrol tables (DT), OVX with YFN (high dose and low dose). After administrating drugs of 4 weeks, sex hormone, NO, NOS, SOD and MDA levels in rat serum were detected.


RESULTS
In ovariectomized rats serum, with the drop of E2 concentration, NO, NOS, SOD levels decreased, while MDA content increased. And the E2 concentration was correlated with other index levels. YFN can obviously regulate above-mentioned indexes.


CONCLUSIONS
The cardiovascular disease occurring in post-menopause rats is possibly due to the decrease of serum sex hormone, which cause the disease through the loss of the NO and damage of the free radical. YFN can prevent and cure the cardiovascular disease by regulating above-mentioned indexes in post-menopause rats.
Xiao
Wei
W
First Military Medicine University, Guangzhou 510515.
Deng
Hongzhu
H
Ma
Yun
Y
Chen
Yuyao
Y
Yang
Jun
J
D000818:Animals; D002214:Capsules; D002318:Cardiovascular Diseases / Q000209:etiology / Q000517:prevention & control; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004967:Estrogens / Q000097:blood*; D005260:Female; D005609:Free Radicals / Q000097:blood*; D008315:Malondialdehyde / Q000097:blood; D008419:Materia Medica / Q000494:pharmacology*; D009569:Nitric Oxide / Q000097:blood*; D019001:Nitric Oxide Synthase / Q000097:blood; D010052:Ovariectomy; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
D002214:Capsules; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D004967:Estrogens; D005609:Free Radicals; D008419:Materia Medica; D009569:Nitric Oxide; D008315:Malondialdehyde; D019001:Nitric Oxide Synthase