New approach for specific determination of antibiotics by use of luminescent Escherichia coli and immune serum.
This paper describes a possible application of luminescent Escherichia coli activated by blood serum for high-sensitivity and high-specificity assays of antibiotics in solutions. Antibiotics inhibited luminescence of a genetically engineered E. coli strain; the system sensitivity to some antibiotics grew notably after the cells had been preactivated by blood serum. The highest level of sensitivity (2.8 +/- 0.6 ng/ml) of luminescent cells was obtained for aminoglycoside antibiotics (gentamicin and streptomycin). It is feasible to create the specific biosensor for antibiotics on the basis of bioluminescent E. coli strains by applying sera containing antibodies against the antibiotic under assay. The presence of antibodies specific for gentamicin in serum affects inhibition of luminescent cells by gentamicin but not inhibition by other antibiotics.
Vlasova
Irina I
II
Department of Microbiology, Faculty of Biology, M. V. Lomonosov Moscow State University, 119899 Moscow, Russia. Irina-Vlasova@newmail.ru
Asrieli
Tatyana V
TV
Gavrilova
Elisaveta M
EM
Danilov
Vadim S
VS
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D007106:Immune Sera; D008163:Luminescent Measurements; D008826:Microbial Sensitivity Tests / Q000379:methods; D011995:Recombination, Genetic; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D007106:Immune Sera