title: Leukemic plasmacytoid dendritic cells share phenotypic and functional features with their normal counterparts.
abstract: This work aims to further characterize the newly described leukemic plasmacytoid dendritic cells (LPDC), for which we had previously demonstrated their normal, PDC-like ability to produce IFN-alpha. In addition, LPDC also express the specific antigens BDCA-2 and BDCA-4. Importantly, they become fully competent antigen-presenting cells (APC) after a short maturation induced by IL-3 + CD40L or virus, exhibiting a characteristic APC phenotype (high expression of CD83 and of the costimulatory molecules CD40, CD80, CD86). Whereas IL-3 + CD40L-activated LPDC prime naive CD4(+) T cells towards a Th2 pathway (IL-4-secreting T cells), virus-activated LPDC drive a Th1 profile (IFN-gamma-secreting T cells). Moreover, we show in one case that LPDC are able to capture, process and present exogenous antigens, leading to the activation of both CD4(+) and CD8(+) T cell clones in an antigen-specific manner. This study further characterizes the phenotype and immunological functions of LPDC.
authors.affiliation: Department of Research and Development, EFS Rhône-Alpes Grenoble, La Tronche, France. laurence.chaperot@efs.sante.fr
authors.forename: Laurence
authors.initials: L
authors.lastname: Chaperot
authors.forename: Ivan
authors.initials: I
authors.lastname: Perrot
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Jacob
authors.forename: Dominique
authors.initials: D
authors.lastname: Blanchard
authors.forename: Véronique
authors.initials: V
authors.lastname: Salaun
authors.forename: Véronique
authors.initials: V
authors.lastname: Deneys
authors.forename: Serge
authors.initials: S
authors.lastname: Lebecque
authors.forename: Francine
authors.initials: F
authors.lastname: Brière
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Bensa
authors.forename: Joël
authors.initials: J
authors.lastname: Plumas
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017951:Antigen Presentation; D000954:Antigens, Surface / Q000276:immunology; D023201:CD40 Ligand / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D002648:Child; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D007377:Interleukin-3 / Q000276:immunology; D007938:Leukemia / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009975:Orthomyxoviridae / Q000276:immunology
chemical_list: D000954:Antigens, Surface; D016207:Cytokines; D007377:Interleukin-3; C478196:blood dendritic cell antigen 4, human; D023201:CD40 Ligand