Development of a serum-based Taqman real-time PCR assay for diagnosis of invasive aspergillosis.
We compared an Aspergillus fumigatus-specific Taqman real-time PCR assay for the diagnosis of invasive aspergillosis (IA) with the enzyme-linked immunosorbent assay Platelia Aspergillus method. Patients with proven or probable IA had positive results with at least one of the two methods. The PCR method seems to be more specific, and a combination of the two should improve the diagnosis of IA.
Challier
Svetlana
S
Laboratoire de Microbiologie, Hôpital Necker-Enfants Malades, Paris, France. svetlana.challier@nck.ap-hop.paris.fr
Boyer
Stéphanie
S
Abachin
Eric
E
Berche
Patrick
P
D000293:Adolescent; D000328:Adult; D000368:Aged; D001228:Aspergillosis / Q000175:diagnosis*; D001232:Aspergillus fumigatus / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results
D017931:DNA Primers