Inducible expression of FGF2 by a rat oligodendrocyte precursor cell line promotes CNS myelination in vitro.
Transplantation of glial cells into the central nervous system (CNS) may be a promising approach for the treatment of myelin disorders such as multiple sclerosis (MS). Myelination by transplantation of oligodendrocyte precursors has been obtained in different animal models of demyelination. A strategy to favor CNS remyelination is to enrich the lesioned areas in growth factors to stimulate the quiescent population of oligodendrocyte precursors. In this context, we have developed a genetically modified CG4 cell line (CG4-FGF2), which are able to release significant amounts of fibroblast growth factor 2 (FGF2) in a controlable fashion in vitro. The data presented here demonstrate that upon induction with Dox, CG4-FGF2 cells retain their capacity to differentiate in vitro. Additionally, we provide evidence that FGF2 release by engineered cells enhance proliferation and migration of cells of the oligodendrocyte lineage without preventing them to differentiate and myelinate axons in vitro.
Magy
Laurent
L
INSERM U546, Laboratoire des Affections de la Myéline et des Canaux Ioniques Musculaires, Faculté de Médecine Pitié-Salpêtrière, IFR 70, CHU Pitié-Salpêtrière, 75634 Paris Cedex 13, France.
Mertens
Caroline
C
Avellana-Adalid
Virginia
V
Keita
Mahamane
M
Lachapelle
François
F
Nait-Oumesmar
Brahim
B
Fontaine
Bertrand
B
Baron-Van Evercooren
Anne
A
D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D009186:Myelin Sheath / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects*; D009836:Oligodendroglia / Q000166:cytology* / Q000378:metabolism*; D051381:Rats; D013234:Stem Cells; D014162:Transfection; D019076:Transgenes
D016222:Fibroblast Growth Factor 2