title: Phosphatidylinositol 4-kinasebeta is critical for functional association of rab11 with the Golgi complex.
abstract: Phosphatidylinositol 4-kinasebeta (PI4Kbeta) plays an essential role in maintaining the structural integrity of the Golgi complex. In a search for PI4Kbeta-interacting proteins, we found that PI4Kbeta specifically interacts with the GTP-bound form of the small GTPase rab11. The PI4Kbeta-rab11 interaction is of functional significance because inhibition of rab11 binding to PI4Kbeta abolished the localization of rab11 to the Golgi complex and significantly inhibited transport of vesicular stomatitis virus G protein from the Golgi complex to the plasma membrane. We propose that a novel function of PI4Kbeta is to act as a docking protein for rab11 in the Golgi complex, which is important for biosynthetic membrane transport from the Golgi complex to the plasma membrane.
authors.affiliation: Department of Molecular Cell Biology and Institute of Biomembranes, Utrecht University, The Netherlands.
authors.forename: Petra
authors.initials: P
authors.lastname: de Graaf
authors.forename: Wilbert T
authors.initials: WT
authors.lastname: Zwart
authors.forename: Remco A J
authors.initials: RA
authors.lastname: van Dijken
authors.forename: Magdalena
authors.initials: M
authors.lastname: Deneka
authors.forename: Thomas K F
authors.initials: TK
authors.lastname: Schulz
authors.forename: Niels
authors.initials: N
authors.lastname: Geijsen
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Coffer
authors.forename: Bart M
authors.initials: BM
authors.lastname: Gadella
authors.forename: Arie J
authors.initials: AJ
authors.lastname: Verkleij
authors.forename: Peter
authors.initials: P
authors.lastname: van der Sluijs
authors.forename: Paul M P
authors.initials: PM
authors.lastname: van Bergen en Henegouwen
mesh_terms: D000818:Animals; D001665:Binding Sites; D001692:Biological Transport; D020126:Brefeldin A / Q000378:metabolism; D019556:COS Cells; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D004247:DNA / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D006160:Guanosine Triphosphate / Q000378:metabolism; D008164:Luminescent Proteins / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry / Q000502:physiology*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D014162:Transfection; D020798:Two-Hybrid System Techniques; D014759:Viral Envelope Proteins / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism; D020691:rab GTP-Binding Proteins / Q000378:metabolism*; D000099193:rab11 GTP-Binding Proteins
chemical_list: D020126:Brefeldin A; D004247:DNA; D018076:DNA, Complementary; D005982:Glutathione Transferase; D049452:Green Fluorescent Proteins; D006160:Guanosine Triphosphate; D008164:Luminescent Proteins; D008562:Membrane Glycoproteins; D017853:Phosphotransferases (Alcohol Group Acceptor); D014759:Viral Envelope Proteins; D001616:beta-Galactosidase; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins; C028391:G protein, vesicular stomatitis virus; C020077:1-phosphatidylinositol-4-phosphate 5-kinase