title: Tannin inhibits HIV-1 entry by targeting gp41.
abstract: OBJECTIVE
  To investigate the mechanism by which tannin inhibits HIV-1 entry into target cells.
  
  
  METHODS
  The inhibitory activity of tannin on HIV-1 replication and entry was detected by p24 production and HIV-1-mediated cell fusion, respectively. The inhibitory activity on the gp41 six-helix bundle formation was determined by an improved sandwich ELISA.
  
  
  RESULTS
  Tannins from different sources showed potent inhibitory activity on HIV-1 replication, HIV-1-mediated cell fusion, and the gp41 six-helix bundle formation.
  
  
  CONCLUSIONS
  Tannin inhibits HIV-1 entry into target cells by interfering with the gp41 six-helix bundle formation, thus blocking HIV-1 fusion with the target cell.
authors.affiliation: Institute of Pharmaceutical Sciences of the First Medical University of PLA, Key Lab of GD for Screening of New Drug, Guangzhou, Guangdong 510515, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Lü
authors.forename: Shu-wen
authors.initials: SW
authors.lastname: Liu
authors.forename: Shi-bo
authors.initials: SB
authors.lastname: Jiang
authors.forename: Shu-guang
authors.initials: SG
authors.lastname: Wu
mesh_terms: D019380:Anti-HIV Agents / Q000494:pharmacology; D002478:Cells, Cultured; D015700:HIV Envelope Protein gp41 / Q000096:biosynthesis*; D023581:HIV Fusion Inhibitors / Q000494:pharmacology*; D015497:HIV-1 / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D047348:Hydrolyzable Tannins / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D019380:Anti-HIV Agents; D015700:HIV Envelope Protein gp41; D023581:HIV Fusion Inhibitors; D047348:Hydrolyzable Tannins