Core-shell nanospheres for oligonucleotide delivery. V: adsorption/release behavior of 'stealth' nanospheres.
The adsorption/release behavior of oligodeoxynucleotides (ODNs) on new PEGylated core-shell polymethylmethacrylate nanospheres is described. The outer shell consists of alkyl chains containing quaternary ammonium groups and of poly(ethylene glycol) chains, both covalently bound to the inner core. Ion pair formation between negatively charged ODN phosphate groups and positively charged groups on the nanosphere surface is the main interaction mechanism. No cellular toxicity in HL60 cells is observed at nanosphere concentrations required for biologically active ODN delivery. These results indicate that these novel cationic polymeric nanoparticles are safe and represent promising vectors for oligonucleotide delivery.
Tondelli
Luisa
L
Consiglio Nazionale delle Ricerche (CNR), Istituto per la Sintesi Organica e la Fotoreattività (ISOF), Via Gobetti 101, 40129 Bologna, Italy. tondelli@isof.cnr.it
Ballestri
Marco
M
Magnani
Laura
L
Vivarelli
Daniela
D
Fini
Adamo
A
Cerasi
Aurora
A
Chiarantini
Laura
L
Sparnacci
Katia
K
Laus
Michele
M
D000327:Adsorption; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations / Q000737:chemistry* / Q000633:toxicity; D018922:HL-60 Cells; D006801:Humans; D008264:Macrophages / Q000166:cytology; D051379:Mice; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology / Q000379:methods; D043942:Nanotubes / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage* / Q000378:metabolism; D010587:Phagocytosis; D013329:Structure-Activity Relationship; D013499:Surface Properties
D003692:Delayed-Action Preparations; D016376:Oligonucleotides, Antisense