title: 3,4-oxo-isopropylidene-shikimic acid inhibits adhesion of polymorphonuclear leukocyte to TNF-alpha-induced endothelial cells in vitro.
abstract: OBJECTIVE
  To examine the effect of 3,4-oxo-isopropylidene-shikimic acid (ISA) on human polymorphonuclear leukocyte (PMN) adhesion to human umbilical vein endothelial cells (HUVEC) and explore its mechanism.
  
  
  METHODS
  Adhesion of PMN to HUVEC was measured by rose bengal staining assay. Cell-ELISA and RT-PCR methods were used to examine the expression of adhesion molecules ICAM-1. Cell viability was detected with MTT assay.
  
  
  RESULTS
  ISA (1-100 micromol/L) effectively reduced PMN adhesion to TNF-alpha-induced HUVEC with the inhibitory rate from 17.2 % to 53.5 %, and exerted no effect on PMN adhesion to normal HUVEC. Adhesion molecule ICAM-1 surface protein and mRNA expression induced by TNF-alpha (400 kU/L) were significantly inhibited by ISA. In addition, the cell viability of HUVEC was unchanged 48 h after treatment with ISA.
  
  
  CONCLUSIONS
  ISA inhibited TNF-alpha-stimulated PMN-HUVEC adhesion and expression of ICAM-1.
authors.affiliation: Department of Pharmacology, Beijing University of Chinese Traditional Medicine, Beijing 100102, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ma
authors.forename: Jian-ning
authors.initials: JN
authors.lastname: Sun
authors.forename: Qiu-ping
authors.initials: QP
authors.lastname: Xu
authors.forename: Zi-li
authors.initials: ZL
authors.lastname: You
authors.forename: Ya-jian
authors.initials: YJ
authors.lastname: Guo
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism / Q000502:physiology*; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000235:genetics; D009504:Neutrophils / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D012765:Shikimic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014471:Umbilical Veins / Q000166:cytology
chemical_list: C479295:3,4-oxoisopropylideneshikimic acid; D005343:Fibrinolytic Agents; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D012765:Shikimic Acid