Comparing behavioral and chronic endpoints to evaluate the response of Lumbriculus variegatus to 3,4,3',4'-tetrachlorobiphenyl sediment exposures.
The response of Lumbriculus variegatus to 3,4,3',4'-tetrachlorobiphenyl (TCBP) was examined with feeding behavior and changes in carbon assimilation by using stable carbon isotopes at 22 and 10 degrees C. The classical measure of feeding behavior determined on a subset of sediment for which the biological burial rate was determined in a companion study allowed direct method comparison. This comparison helped address relationships between biological burial rate, feeding rate, and bioaccumulation. The change in stable isotope composition reflects the total metabolic activity by measuring carbon assimilation rate and was compared to feeding rate, biological burial rate (as determined in the companion study), and reproduction. Decreasing the temperature from 22 to 10 degrees C resulted in a twofold reduction in feeding rate and carbon assimilation. The fractional decline in feeding rate relative to the control mimicked the decline in the biological burial rate with increasing TCBP concentration that was found in the companion study. The bioaccumulation factor declined with increasing TCBP sediment concentration, tracking the feeding rate decline. Stable isotope measures showed differences in metabolic rates between the exposure temperatures but did not distinguish a metabolic rate change at 22 degrees C among TCBP treatments. Likewise, reproduction declined from 22 to 10 degrees C, with no reproduction at 10 degrees C. Like the stable isotope measure, no dose response was found among TCBP treatments at 22 degrees C. The reduction in carbon assimilation rate tracked the reduction in reproduction with lower temperature.
Landrum
Peter F
PF
Great Lakes Environmental Research Laboratory, NOAA/GLERL, 2205 Commonwealth Boulevard, Ann Arbor, Michigan 48105, USA. peter.landrum@noaa.gov
Leppänen
Matti
M
Robinson
Sander D
SD
Gossiaux
Duane C
DC
Burton
G Allen
GA
Greenberg
Marc
M
Kukkonen
Jussi V K
JV
Eadie
Brian J
BJ
Lansing
Margaret B
MB
D000818:Animals; D002244:Carbon / Q000378:metabolism; D002247:Carbon Isotopes / Q000032:analysis; D023381:Endpoint Determination; D004781:Environmental Exposure; D004785:Environmental Pollutants / Q000633:toxicity*; D005247:Feeding Behavior / Q000187:drug effects*; D019015:Geologic Sediments; D009068:Movement; D009835:Oligochaeta; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D013696:Temperature; D014018:Tissue Distribution
D002247:Carbon Isotopes; D004785:Environmental Pollutants; D002244:Carbon; D011078:Polychlorinated Biphenyls; C028451:3,4,3',4'-tetrachlorobiphenyl