[A new regulatory function of the A-factor--stimulation of the streptomyces spore germination].
Spore germination in streptomycetes was shown to be stimulated by exogenously added A-factor. Agar medium either containing or not containing A-factor was inoculated with spore suspensions of three strains differing in their ability to produce regulators of the A-factor group: Streptomyces griseus 773, which produces A-factor and two its lower homologs, S. coelicolor A3(2), which forms six AcL-factors (A-factor analogues), and S. avermitilis JCM5070, which fails to form regulators of this group. The count of the grown colonies showed that exogenous A-factor stimulated spore germination in strains that were themselves able to synthesize regulators of the A-factor group. In S. griseus 773, the number of germinated spores increased by 67% on average after the addition A-factor to the medium in an amount 10 micrograms/ml. In strain S. coelicolor A3 (2), the number of germinated spores increased by 75% after the addition of 1 microgram/ml of A-factor. During germination of the S. avermitilis JCM5070 spores, no changes in the CFU number was observed after the addition of A-factor.
Gruzina
V D
VD
Gauze Institute of New Antibiotics, Russian Academy of Medical Sciences, ul. Bol'shaya Pirogovskaya 11, Moscow, Russia.
Gorbatiuk
E V
EV
Efremenkova
O V
OV
Filippova
S N
SN
El'-Registan
G I
GI
Dudnik
Iu V
IuV
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D013045:Species Specificity; D013171:Spores, Bacterial / Q000187:drug effects / Q000254:growth & development; D013306:Streptomycetaceae / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism
C025035:A-factor (Streptomyces); D015107:4-Butyrolactone