Genetic analysis of an ambler class A extended-spectrum beta-lactamase from Capnocytophaga ochracea.
A beta-lactamase gene (cfxA3, 966 bp) was isolated from a beta-lactam-resistant Capnocytophaga ochracea clinical isolate and amplified using primers from the cfxA gene of Bacteroides vulgatus. The MICs of third-generation cephalosporins were much higher than those of the transconjugant Escherichia coli strain. The deduced protein sequence, by comparison with CfxA2 of Prevotella intermedia, had a Y239D substitution and possessed the characteristics of a class A, group 2e beta-lactamase.
Jolivet-Gougeon
Anne
A
Laboratoire de Microbiologie Pharmaceutique, UPRES-EA 1254, Université de Rennes 1, France. anne.gougeon@univ-rennes1.fr
Tamanai-Shacoori
Zohreh
Z
Desbordes
Laurent
L
Burggraeve
Nathalie
N
Cormier
Michel
M
Bonnaure-Mallet
Martine
M
D019943:Amino Acid Substitution; D001483:Base Sequence; D017931:DNA Primers; D006090:Gram-Negative Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D016867:Immunocompromised Host; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping; D018805:Sepsis / Q000453:epidemiology / Q000382:microbiology; D001618:beta-Lactamases / Q000032:analysis / Q000235:genetics*
D017931:DNA Primers; C462979:cfxA protein, Prevotella; D001618:beta-Lactamases