Specific glycolipid antigen(s) in SV40-transformed cell membranes.
A glycolipid extract was prepared from an SV40-transformed hamster cell line (EH-SV) according to the Folch partition procedure. The glycolipids from the aqueous layer were incorporated in liposomal membranes composed of lecithin/sphingomyelin/cholesterol (1:1:2 by weight). This liposomal preparation was inoculated in Syrian hamsters to raise immune sera. The sera were absorbed with trypsinized "normal" hamster cells (EH-N) and tested on various cell lines by the indirect immunofluorescence technique. When used for staining living cells, the immune serum produced a distinct cell-surface fluorescence with SV40-transformed cell lines regardless of the cell origin (e.g., rat or hamster). No reaction was observed with heterologous Py-transformed cell lines, spontaneously transformed cells, or sera from non-immunized hamsters. When used for staining acetone-fixed cells, the antiglycolipid serum reacted specifically with a thermostable antigen in the nuclear envelope and the cytoplasm of SV40-transformed cells. The sera lack interfering SV40 T reactivity. The results indicate the presence of related SV40-specific glycolipid antigen(s) in the plasma membrane, the nuclear membrane and probably other endomembranes of SV40-transformed cells.
Ansel
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Institut de Recherches Scientifiques sur le Cancer, B.P. No. 8, 94800, Villejuif, France.
Huet
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D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D002461:Cell Line, Transformed; D002462:Cell Membrane / Q000648:ultrastructure*; D002472:Cell Transformation, Viral; D006224:Cricetinae; D019084:Fluorescent Antibody Technique, Indirect; D006017:Glycolipids / Q000032:analysis*; D006028:Glycosphingolipids / Q000032:analysis; D008081:Liposomes; D008563:Membrane Lipids / Q000032:analysis; D008647:Mesocricetus; D051381:Rats; D013539:Simian virus 40 / Q000235:genetics*
D000956:Antigens, Viral; D006017:Glycolipids; D006028:Glycosphingolipids; D008081:Liposomes; D008563:Membrane Lipids