title: Neuronal and glial differentiation within expanded glial cultures derived from the lateral and medial ganglionic eminences.
abstract: Attached glial-like cell cultures were established from the lateral and medial ganglionic eminences (LGE and MGE) and from the neocortex (Cx) of E13.5 mouse embryos, and expanded over four to five passages under epidermal growth factor (EGF) stimulation. Following removal of EGF and serum, we analysed the generation of neurons and glial cells within the cultures. Significant numbers of betaIII-tubulin-positive neurons were generated in both the LGE (about 7% of total cell numbers) and the MGE (around 2%). However, only few betaIII-tubulin-positive cells with neuronal morphologies were detected in the differentiated Cx cultures. The newly formed neurons were to a large extent GABAergic, and many of the MGE-derived, but not the LGE-derived, cells expressed the MGE-marker NKX2.1. Most cells in all cultures still appeared astroglial-like, expressing glial fibrillary acidic protein (GFAP), but in addition, CNPase-positive cells with oligodendroglial morphologies were present in the MGE (0.68%), and, to a lesser extent (0.2%), in the LGE cultures. The present results demonstrate that cells of expanded glial cultures from both the LGE and MGE can give rise to significant and, to a certain extent, region-specific neuronal and glial cell types under differentiating conditions.
authors.affiliation: Wallenberg Neuroscience Center, Division of Neurobiology, Lund University, Lund, Sweden. Cicci.Eriksson@mphy.lu.se
authors.forename: Cecilia
authors.initials: C
authors.lastname: Eriksson
authors.forename: Klas
authors.initials: K
authors.lastname: Wictorin
mesh_terms: D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D008473:Median Eminence / Q000166:cytology; D051379:Mice; D008822:Mice, Transgenic; D019579:Neocortex / Q000166:cytology; D009457:Neuroglia / Q000166:cytology*; D009474:Neurons / Q000166:cytology*; D009687:Nuclear Proteins / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology*; D013687:Telencephalon / Q000166:cytology*; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D005904:Glial Fibrillary Acidic Protein; C000614414:Nkx2-1 protein, mouse; D009687:Nuclear Proteins; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors; D014404:Tubulin; C462941:beta3 tubulin, mouse; D005680:gamma-Aminobutyric Acid; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases