[The detection of microsatellite instability by ion-pair reversed-phase high performance liquid chromatography].
OBJECTIVE
To set up a sensitive and stable technique which has high throughout to detect the instability of microsatellite DNA.


METHODS
Genomic DNA extracted from the cancer tissues and their normal tissues were subjected to microsatellite instability(MSI) analysis on five of DNA markers in 115 sporadic colorectal cancers by means of PCR and ion-pair reversed-phase high performance liquid chromatography. Genomic DNA extracted from lymphocytes in blood of 20 normal persons were analysed and used as the standard control.


RESULTS
Seventeen (14.8%) MSI-H and 23(20.0%) MSI-L were found in 115 sporadic colorectal cancers. The rates of MSI in the young patients and old patients were much higher than that in the middle-age patients (P<0.05). And the rate of MSI in low differentiation group was also much higher than that in high or middle differentiation groups (P<0.05).


CONCLUSIONS
The method the authors developed is a sensitive and accurate technique to detect MSI and has a high throughput.
Liu
Xiao-rong
XR
Department of Molecular Biology, Jiangsu Institute of Cancer Research, Nanjing, Jiangsu, PR China.
Wang
Ya-ping
YP
Zhang
Jun-ni
JN
Li
Jin-tian
JT
Zhang
Xiao-mei
XM
Zhu
Ming
M
Shan
Xiang-nian
XN
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology; D003123:Colorectal Neoplasms, Hereditary Nonpolyposis / Q000235:genetics / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics*; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged; D012004:Rectal Neoplasms / Q000235:genetics / Q000473:pathology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004273:DNA, Neoplasm