title: Core-shell nanospheres for oligonucleotide delivery. V: adsorption/release behavior of 'stealth' nanospheres.
abstract: The adsorption/release behavior of oligodeoxynucleotides (ODNs) on new PEGylated core-shell polymethylmethacrylate nanospheres is described. The outer shell consists of alkyl chains containing quaternary ammonium groups and of poly(ethylene glycol) chains, both covalently bound to the inner core. Ion pair formation between negatively charged ODN phosphate groups and positively charged groups on the nanosphere surface is the main interaction mechanism. No cellular toxicity in HL60 cells is observed at nanosphere concentrations required for biologically active ODN delivery. These results indicate that these novel cationic polymeric nanoparticles are safe and represent promising vectors for oligonucleotide delivery.
authors.affiliation: Consiglio Nazionale delle Ricerche (CNR), Istituto per la Sintesi Organica e la Fotoreattività (ISOF), Via Gobetti 101, 40129 Bologna, Italy. tondelli@isof.cnr.it
authors.forename: Luisa
authors.initials: L
authors.lastname: Tondelli
authors.forename: Marco
authors.initials: M
authors.lastname: Ballestri
authors.forename: Laura
authors.initials: L
authors.lastname: Magnani
authors.forename: Daniela
authors.initials: D
authors.lastname: Vivarelli
authors.forename: Adamo
authors.initials: A
authors.lastname: Fini
authors.forename: Aurora
authors.initials: A
authors.lastname: Cerasi
authors.forename: Laura
authors.initials: L
authors.lastname: Chiarantini
authors.forename: Katia
authors.initials: K
authors.lastname: Sparnacci
authors.forename: Michele
authors.initials: M
authors.lastname: Laus
mesh_terms: D000327:Adsorption; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations / Q000737:chemistry* / Q000633:toxicity; D018922:HL-60 Cells; D006801:Humans; D008264:Macrophages / Q000166:cytology; D051379:Mice; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology / Q000379:methods; D043942:Nanotubes / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage* / Q000378:metabolism; D010587:Phagocytosis; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D003692:Delayed-Action Preparations; D016376:Oligonucleotides, Antisense