[Heterocysts with reduced cell walls in populations of cycad cyanobionts].
The ultrastructure of the cyanobionts of the greenhouse-grown cycads Cycads circinalis, Ceratozamia mexicana, and Encephalartos villosus was studied. In addition to heterocysts with the typical ultrastructure, the cyanobiont microcolonies also contained altered heterocysts with reduced cell walls, which might dominate in all regions of the coralloid roots. The altered heterocysts represented a protoplast enclosed in a heterocyst-specific envelope with additional layers. Some heterocysts contained an additional reticular protoplast-enclosing sheath below the heterocyst-specific envelope, whereas the other heterocysts contained an additional electron-opaque outer layer. The substance of the inner sheath of the former heterocysts resembled the polysaccharides of mucilage, which fills the intercellular space of plant tissues, whereas the electron-opaque outer layer of the latter heterocysts probably had a protein nature. The substances that constitute the sheath and the outer layer are likely to be synthesized intracellularly and then released with the aid of membrane-bounded vesicles or by channels in the cytoplasmic membrane.
Baulina
O I
OI
Faculty of Biology, Moscow State University, Vorob'evy gory, Moscow, 119992 Russia.
Lobakova
E S
ES
D002473:Cell Wall; D000458:Cyanobacteria / Q000502:physiology* / Q000648:ultrastructure; D032462:Cycadopsida / Q000382:microbiology / Q000502:physiology*; D008854:Microscopy, Electron; D018517:Plant Roots / Q000382:microbiology; D013559:Symbiosis