[Effect of ligustrazine on cell cycle and prostacyclin of injured vascular endothelial cells induced by hypoxia and lack of glucose].
OBJECTIVE
To study the effects of ligustrazine on cell cycle and prostacyclin of injured human umbilical vein vascular endothelial cell line induced by hypoxia and lack of glucose.


METHODS
The experiments were performed by culturing vascular endothelial cell induced by hypoxia and lack of glucose in vitro. Cell viability was assessed by MTT assay and cell cycle was observed by flow cytometry. Radioimmunoassay (RIA) was used to assess the amount of prostacyclin (PGI2).


RESULTS
The vascular endothelial cell viability and PGI2 were decreased in condition of hypoxia and lack of glucose-injury. Preincubation of vascular endothelial cell with Ligustrazine for 24 h significantly increased the cell viability, S-Phase cell, G2M-phase cell and PGI2 level.


CONCLUSIONS
These results demonstrated that ligustrazine had protective effect on hypoxia and lack of glucose-injured vascular endothelial cell and the effect may be related to increasing cell viability, S-phase cell, G2M-phase cell and level of PGI2.
Lin
Rong
R
Medical School of Xi'an Jiaotong University, Xi'an 710061.
Liu
Juntian
J
Gan
Weijie
W
D002453:Cell Cycle / Q000187:drug effects*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D005947:Glucose; D044062:Prostaglandins I / Q000096:biosynthesis*; D011719:Pyrazines / Q000494:pharmacology*; D011863:Radioimmunoassay
D004365:Drugs, Chinese Herbal; D044062:Prostaglandins I; D011719:Pyrazines; D005947:Glucose; C017953:tetramethylpyrazine