Phosphatidylinositol 4-kinasebeta is critical for functional association of rab11 with the Golgi complex.
Phosphatidylinositol 4-kinasebeta (PI4Kbeta) plays an essential role in maintaining the structural integrity of the Golgi complex. In a search for PI4Kbeta-interacting proteins, we found that PI4Kbeta specifically interacts with the GTP-bound form of the small GTPase rab11. The PI4Kbeta-rab11 interaction is of functional significance because inhibition of rab11 binding to PI4Kbeta abolished the localization of rab11 to the Golgi complex and significantly inhibited transport of vesicular stomatitis virus G protein from the Golgi complex to the plasma membrane. We propose that a novel function of PI4Kbeta is to act as a docking protein for rab11 in the Golgi complex, which is important for biosynthetic membrane transport from the Golgi complex to the plasma membrane.
de Graaf
Petra
P
Department of Molecular Cell Biology and Institute of Biomembranes, Utrecht University, The Netherlands.
Zwart
Wilbert T
WT
van Dijken
Remco A J
RA
Deneka
Magdalena
M
Schulz
Thomas K F
TK
Geijsen
Niels
N
Coffer
Paul J
PJ
Gadella
Bart M
BM
Verkleij
Arie J
AJ
van der Sluijs
Peter
P
van Bergen en Henegouwen
Paul M P
PM
D000818:Animals; D001665:Binding Sites; D001692:Biological Transport; D020126:Brefeldin A / Q000378:metabolism; D019556:COS Cells; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D004247:DNA / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D006160:Guanosine Triphosphate / Q000378:metabolism; D008164:Luminescent Proteins / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry / Q000502:physiology*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D014162:Transfection; D020798:Two-Hybrid System Techniques; D014759:Viral Envelope Proteins / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism; D020691:rab GTP-Binding Proteins / Q000378:metabolism*; D000099193:rab11 GTP-Binding Proteins
D020126:Brefeldin A; D004247:DNA; D018076:DNA, Complementary; D005982:Glutathione Transferase; D049452:Green Fluorescent Proteins; D006160:Guanosine Triphosphate; D008164:Luminescent Proteins; D008562:Membrane Glycoproteins; D017853:Phosphotransferases (Alcohol Group Acceptor); D014759:Viral Envelope Proteins; D001616:beta-Galactosidase; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins; C028391:G protein, vesicular stomatitis virus; C020077:1-phosphatidylinositol-4-phosphate 5-kinase