Using single-strand conformational polymorphism gel electrophoresis to analyze mutually exclusive alternative splicing.
Single-strand conformational polymorphism analysis has been used successfully to identify single nucleotide changes within sequences based on the fact that multidetection enhancement gels will separate molecules based on their conformation rather than their size. We have expanded the utility of this technique to analyze easily the alternative splicing of pre-mRNAs containing multiple mutually exclusive exons of the same size. We have used this technique to study the Caenorhabditis elegans let-2 gene containing two alternative exons and the Drosophilia melanogaster Dscam gene, which contains 12 mutually exclusive exons. The ease and the quantitative nature of this technique should be very useful.
Celotto
Alicia M
AM
Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT, USA.
Graveley
Brenton R
BR
D017398:Alternative Splicing / Q000502:physiology*; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000378:metabolism; D015815:Cell Adhesion Molecules; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D004586:Electrophoresis / Q000379:methods*; D005091:Exons / Q000235:genetics; D017238:Genes, Helminth / Q000502:physiology*; D007438:Introns / Q000235:genetics; D018807:Polymorphism, Single-Stranded Conformational; D020033:Protein Isoforms; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification*; D012322:RNA Precursors / Q000235:genetics
D015815:Cell Adhesion Molecules; D029721:Drosophila Proteins; C491736:Dscam1 protein, Drosophila; D020033:Protein Isoforms; D011506:Proteins; D012322:RNA Precursors