In vivo effect of vitamin C with cobalt on oxidative stress in experimental diabetic rat kidney.
In the present study, kidney superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) activities, vitamin C and lipid peroxidation levels were investigated in diabetic rats. Diabetes was induced in rats by streptozotocin and the treated rats received 1 g/l vitamin C with 0.5 mM CoCl2 in drinking water at 2-week intervals for 6 weeks. Kidney SOD, GSH-Px, CAT activities and lipid peroxidation levels were significantly increased in diabetic rats at the end of the 2nd, 4th and 6th weeks (p < 0.05), whereas vitamin C level was decreased significantly (p < 0.05) at the end of the 6th week compared to those of controls. Vitamin C with cobalt treatment of diabetic rats resulted in partial restoration of SOD and CAT activities, thiobarbituric acid reactant substances and vitamin C levels at all times studied, whereas treatment did not change GSH-Px activity. These results suggest that vitamin C with cobalt effectively normalized hyperglycemia (at the end of the 6th week) but could not completely restore the altered endogenous defence systems in diabetic rat kidney.
Yildirim
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Faculty of Science, Department of Biology, Ankara University, Tandogan, Ankara, Turkey. ozlemesn@hotmail.com
Büyükbingöl
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D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage* / Q000097:blood; D002374:Catalase / Q000378:metabolism; D003035:Cobalt / Q000008:administration & dosage*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D017392:Thiobarbituric Acid Reactive Substances; D003035:Cobalt; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001205:Ascorbic Acid