Galectin-1 induces cell adhesion to the extracellular matrix and apoptosis of non-adherent human colon cancer Colo201 cells.
To isolate cDNAs for molecules involved in cell adhesion to the extracellular matrix, expression cloning with non-adherent colon cancer Colo201 cells was carried out. Four positive clones were isolated and, when sequenced, one was found to be galectin-1, a beta-galactoside-binding protein. When cultured on fibronectin-, laminin-, and collagen-coated and non-coated dishes, the adherent galectin-1 cDNA-transfected Colo201 cells increased and spread somewhat. Immunofluorescence staining revealed that galectin-1 was expressed inside and outside of Colo201 cells. The adhesion was dependent on the carbohydrate-recognition domain of galectin-1 since lactose inhibited the adhesion and exogenously-added galectin-1 caused the adhesion. PD58059, an inhibitor of mitogen-activated protein kinase, or LY294002, a phosphoinositide 3-OH kinase inhibitor, decreased the adhesion. Furthermore, the expression of galectin-1 in Colo201 cells induced apoptotic cell death, while exogenously-added galectin-1 did not cause apoptosis. These results indicate that galectin-1 plays a role in both cell-matrix interactions and the inhibition of Colo201 cell proliferation, and suggest that galectin-1 expressed in cells could be associated with apoptosis.
Horiguchi
Natsuko
N
Department of Biotechnology, Kyoto Institute of Technology, Kyoto 606-8585.
Arimoto
Kei-ichiro
K
Mizutani
Atsushi
A
Endo-Ichikawa
Yoko
Y
Nakada
Hiroshi
H
Taketani
Shigeru
S
D017209:Apoptosis / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000302:isolation & purification / Q000502:physiology; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism / Q000502:physiology; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D016326:Extracellular Matrix Proteins / Q000502:physiology*; D037483:Galectin 1 / Q000096:biosynthesis / Q000502:physiology*; D006131:Growth Inhibitors / Q000096:biosynthesis / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis / Q000502:physiology; D014162:Transfection
D015815:Cell Adhesion Molecules; D016326:Extracellular Matrix Proteins; D037483:Galectin 1; D006131:Growth Inhibitors; D008565:Membrane Proteins