title: Characterization of cyst cell formation in the purple photosynthetic bacterium Rhodospirillum centenum.
abstract: Rhodospirillum centenum is an anoxygenic photosynthetic bacterium that is capable of differentiating into several cell types. When grown phototrophically in liquid, cells exhibit a vibrioid shape and have a single polar flagellum. When grown on a solid surface, R. centenum will differentiate into rod-shaped swarm cells that display numerous lateral flagella. Upon starvation for nutrients, R. centenum also forms desiccation-resistant cysts. In this study, it was determined that R. centenum has heat- and desiccation-resistance properties similar to other cyst-forming species. In addition, microscopic analyses of the morphological changes that occur during cyst cell development were performed. It was observed that R. centenum typically forms multi-celled clusters of cysts that contain from four to more than 10 cells per cluster. It was also determined that cell density has a minor effect on the percentage of cyst cells formed, with cell densities of 10(5)-10(7) cells per 5 micro l spot yielding the highest percentage of cyst cells. The striking similarities between the life cycle of R. centenum and the life cycle exhibited by Azospirillum spp. are discussed.
authors.affiliation: Department of Biology, Indiana University, Jordan Hall, Bloomington, IN 47405, USA.
authors.forename: James E
authors.initials: JE
authors.lastname: Berleman
authors.affiliation: Department of Biology, Indiana University, Jordan Hall, Bloomington, IN 47405, USA.
authors.forename: Carl E
authors.initials: CE
authors.lastname: Bauer
mesh_terms: D000064:Acclimatization; D002473:Cell Wall / Q000502:physiology; D003890:Desiccation; D006358:Hot Temperature; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D010788:Photosynthesis; D042182:Rhodospirillum centenum / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D013997:Time Factors