Hydrogen bonding between histidine and lignin model compounds or redox mediators as calculated with the DFT method. Effects on the ease of oxidation.
Using the Density Functional Theory method, the effect of hydrogen bonding between imidazole (IM) and ten benzyl alcohol derivatives (BA) on the ionization potentials of the latter is calculated. IM is used as a model for histidine, which is found in the reaction sites of laccases and lignin peroxidases, and the BA-derivatives serve as lignin model compounds. A marked decrease ([similar]15 kcal mol(-1)) is found for the IP's of the BA-derivatives when paired with IM. This should facilitate the one-electron oxidation of BA in the reaction site of the enzyme. The same effect is found for the known redox mediators violuric acid, 1-hydroxybenzotriazole and N-hydroxyacetanilide which are assumed to enter the reaction site of the enzymes. Furthermore, upon one-electron oxidation the strength of the H-bond from BA to IM is considerably increased and in the case of the mediators this effect is so pronounced that the relevant proton shifts from them to IM. If this occurs in the active site of the enzyme then the oxidized redox mediators are released into the aqueous phase in their neutral form rather than as radical cations (deprotonation of the radical cations). The oxidation power of the neutral radical mediators, however, is too low to initialize oxidation of lignin. A more likely reaction pathway is oxidation of the substrates via hydrogen abstraction. The pertinent bond dissociation energies are similar for the BA-derivatives and the redox mediators, which in principle allows the reaction to occur.
Reynisson
Jóhannes
J
Max-Planck-Institut für Strahlenchemie, D-45413, Mülheim, Germany. Johannes Reynisson@icr.ac.uk
Steenken
Steen
S
D019281:Dimerization; D006639:Histidine / Q000737:chemistry*; D006860:Hydrogen Bonding; D007477:Ions / Q000737:chemistry; D008031:Lignin / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D010084:Oxidation-Reduction
D007477:Ions; D006639:Histidine; D008031:Lignin