title: [The relationship between the variants in 5' upstream core promoter A(-6)G and A(-20)C of angiotensinogen gene and essential hypertension in Kazakans of Xinjiang].
abstract: OBJECTIVE
  To investigate whether the variants A(-6)G and A(-20)C of angiotensinogen (AGT) gene are involved in the pathogenesis of essential hypertension in Kazakans.
  
  
  METHODS
  T his case control study recruited 125 subjects with hypertension and 74 normotensive subjects from Kazakans of Xinjiang. Genomic DNA from leukocytes was analyzed for genetic variants A(-6)G and A(-20)C in 5' upstream core promoter of AGT gene by polymerase chain reaction (PCR), single strand conformation polymorphism (SSCP), restriction fragment length polymorphism (RFLP) and automatic sequencing.
  
  
  RESULTS
  (1)There were only A(-6)G and A(-20)C variants in the -164 to +73 region of Kazakans' AGT gene. (2) The distributions of genotypes AA, AG, GG at locus -6 of AGT gene showed significant difference between the hypertensive group (0.39, 0.45, 0.16) and the normotensive group (0.49, 0.49, 0.02; Chi2=8.56, P=0.014). There were evident differences in the frequencies of the -6A and the -6G allele of the two groups (0.62, 0.38 and 0.73, 0.27; Chi2=5.35, P=0.021). (3) No significant difference was observed in the distribution of genotypes AA, AC, CC at locus -20 of AGT gene between the hypertensive group (0.69, 0.26, 0.05) and the normotensive group (0.65, 0.32, 0.03; Chi2=2.42, P=0.30). There was no distinct difference in the frequencies of the -20A allele and the -20C allele of the two groups (0.82, 0.18 and 0.82, 0.18; Chi2=0, P=0.99). (4) No significant difference was found at the levels of systolic and diastolic blood pressure between the groups corresponding to genotypes at the loci -6 and -20 of AGT gene.
  
  
  CONCLUSIONS
  The results suggest that the polymorphism of A(-6)G in 5' upstream core promoter of the AGT gene may be involved in the pathogenesis of essential hypertension in Kazakans, while the A(-20)C variant may not play an important role in the etiology of essential hypertension in Kazakans.
authors.affiliation: Hypertension Unit, the People's Hospital of the Xinjiang Uygur Autonomous Region, Urumuqi, Xinjiang, PR China.
authors.forename: Nan-fang
authors.initials: NF
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Zhou
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Wu
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.forename: Xin-ling
authors.initials: XL
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Hong-jian
authors.initials: HJ
authors.lastname: Li
authors.forename: De-lian
authors.initials: DL
authors.lastname: Zhang
authors.forename: Here
authors.initials: H
authors.lastname: Zu
authors.forename: Wei-jin
authors.initials: WJ
authors.lastname: Ouyang
authors.forename: Ke-li
authors.initials: KL
authors.lastname: Bu
authors.forename: Ke-ming
authors.initials: KM
authors.lastname: Zhou
authors.forename: Qiu-yan
authors.initials: QY
authors.lastname: Cheng
authors.forename: Nur
authors.initials: N
authors.lastname: Guli
authors.forename: Deng-hao
authors.initials: DH
authors.lastname: Zhu
mesh_terms: D024506:5' Flanking Region / Q000235:genetics*; D000328:Adult; D000483:Alleles; D000808:Angiotensinogen / Q000235:genetics*; D001483:Base Sequence; D001794:Blood Pressure / Q000502:physiology; D016022:Case-Control Studies; D002681:China; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004252:DNA Mutational Analysis; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000235:genetics* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D000808:Angiotensinogen; D004247:DNA