[Animal model and modified technique of orthotopic liver transplantation in rats].
OBJECTIVE
To modify orthotopic liver transplantation method with two-cuff technique.


METHODS
On the basis of cuff technique, the donor liver was perfused through the abdominal aorta with 20 ml cold perfusate. The anastomosis of the suprahepatic vena cave(SVC) was sutured end-to-end with 8/0 nylon line, and the continuity of infrahepatic vena cave(IVC) and portal vein(PV) were established by means of cuff method respectively. The bile duct anastomosis was performed by internal stent.


RESULTS
Orthotopic liver transplantations were performed in 360 rats. The time for donor operation and liver preparation was (31.2 +/- 5.0) min and (12.0 +/- 3.0) min, and the recipient operative and anhepatic time was (45.0 +/- 5.5) min and (20.0 +/- 2.5) min, respectively. The two-day survival rate was 91.4%. In non-intervention group, one-week survival rate was 86.5%.


CONCLUSIONS
The modified model is easily available and highly reproducible which provides a practical and stable experimental model for the study of liver transplantation.
Li
Zong-kuang
ZK
Department of General Surgery, Affiliated Hospital, Bengbu Medical College, Bengbu, Anhui, P. R. China 233004.
Ma
Yi
Y
Xu
Chi
C
D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D001652:Bile Ducts / Q000601:surgery; D004195:Disease Models, Animal; D006085:Graft Survival; D008099:Liver / Q000098:blood supply*; D016031:Liver Transplantation / Q000379:methods*; D008297:Male; D011169:Portal Vein / Q000601:surgery; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D019651:Plastic Surgery Procedures