Immunohistochemical study of distribution of apolipoproteins E and D in human cerebral beta amyloid deposits.
Several molecules are known to be closely associated with amyloid deposits in human brain. Among these, apolipoproteins such as apolipoproteins E (apo E) and J (apo J) have been found in two neuropathological hallmarks of Alzheimer's disease (AD) and cerebral amyloid angiopathy (CAA): senile plaques (SPs) and cerebrovascular amyloid. These apolipoproteins may be implicated in amyloid fibrillogenesis. Apo D is a multiligand-multifunctional glycoprotein present in SPs, as we previously reported. The aim of this work is to study the link between immunolocalization of apo E and apo D in AD and CAA brains. Both apolipoproteins were found in all types of SPs, but apo E was observed more often than apo D in mature plaques. Whereas apo E is always located overlapping the amyloid core, apo D seems to situate preferably around and near the amyloid. Immunohistochemistry revealed that these apolipoproteins behave differently in cerebral vessels. Apo E labeling in vessels appears mainly linked to amyloid deposits, whereas apo D shows a distribution almost opposite to that of apo E. This could be an indication of the different roles that each apolipoprotein plays in the pathogenesis of amyloid deposition.
Navarro
Ana
A
Departamento de Morfología y Biología Celular, Facultad de Biología y Medicina, Universidad de Oviedo, Oviedo 33006, Spain.
Del Valle
Eva
E
Astudillo
Aurora
A
González del Rey
Carmen
C
Tolivia
Jorge
J
D000368:Aged; D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D001053:Apolipoproteins / Q000378:metabolism*; D053399:Apolipoproteins D; D001057:Apolipoproteins E / Q000378:metabolism*; D016657:Cerebral Amyloid Angiopathy / Q000378:metabolism / Q000473:pathology; D002540:Cerebral Cortex / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D058225:Plaque, Amyloid / Q000378:metabolism / Q000473:pathology
D016229:Amyloid beta-Peptides; D001053:Apolipoproteins; D053399:Apolipoproteins D; D001057:Apolipoproteins E