title: Novel method using a laser scanning cytometer for detection of mycobacteria in clinical samples.
abstract: In order to evaluate the capacity of laser scanning cytometry (LSC) to detect acid-fast bacilli directly on clinical samples, a comparison between Kinyoun-stained smears analyzed under light microscopy and propidium iodide-auramine-stained smears analyzed by LSC was performed. The results were compared with those for culture on BACTEC MGIT 960. LSC is a new, reliable methodology to detect MYCOBACTERIA:
authors.affiliation: Department of Microbiology, Faculty of Medicine, University of Porto, Hospital S João, Porto, Portugal. micteam@clix.pt
authors.forename: Cidália
authors.initials: C
authors.lastname: Pina-Vaz
authors.forename: Sofia
authors.initials: S
authors.lastname: Costa-Oliveira
authors.forename: Acácio Gonçalves
authors.initials: AG
authors.lastname: Rodrigues
authors.forename: Alexandre
authors.initials: A
authors.lastname: Salvador
mesh_terms: D003592:Cytophotometry / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D018613:Microscopy, Confocal / Q000379:methods; D009161:Mycobacterium / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000175:diagnosis*; D013048:Specimen Handling / Q000379:methods
chemical_list: D007202:Indicators and Reagents