A new alkali-thermostable azoreductase from Bacillus sp. strain SF.
A screening for dye-decolorizing alkali-thermophilic microorganisms resulted in a Bacillus sp. strain isolated out of the wastewater drain of a textile finishing company. An NADH-dependent azoreductase of this strain, Bacillus sp. strain SF, was found to be responsible for the decolorization of azo dyes. This enzyme was purified by a combination of ammonium sulfate precipitation and anion-exchange and affinity chromatography and had a molecular mass of 61.6 kDa and an isoelectric point at pH 5.3. The pH optimum of the azoreductase depended on the substrate and was within the range of pHs 8 to 9, while the temperature maximum was reached at 80 degrees C. Decolorization only took place in the absence of oxygen and was enhanced by FAD, which was not consumed during the reaction. A 26% similarity of this azoreductase to chaperonin Cpn60 from a Bacillus sp. was found by peptide mass mapping experiments. Substrate specificities of the azoreductase were studied by using synthesized model substrates based on di-sodium-(R)-benzyl-azo-2,7-dihydroxy-3,6-disulfonyl-naphthaline. Those dyes with NO2 substituents, especially in the ortho position, were degraded fastest, while analogues with a methyl substitution showed the lowest degradation rates.
Maier
Jürgen
J
Department of Environmental Biotechnology, Graz University of Technology, A-8010 Graz, Austria.
Kandelbauer
Andreas
A
Erlacher
Angelika
A
Cavaco-Paulo
Artur
A
Gübitz
Georg M
GM
D001391:Azo Compounds / Q000737:chemistry / Q000378:metabolism; D001407:Bacillus / Q000201:enzymology*; D001673:Biodegradation, Environmental; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D009243:NAD / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D009601:Nitroreductases; D010449:Peptide Mapping; D013379:Substrate Specificity; D013696:Temperature; D013783:Textile Industry; D014865:Waste Disposal, Fluid
D001391:Azo Compounds; D004396:Coloring Agents; D009243:NAD; D009247:NADH, NADPH Oxidoreductases; D009601:Nitroreductases; C021106:azoreductase