title: Development of a serum-based Taqman real-time PCR assay for diagnosis of invasive aspergillosis.
abstract: We compared an Aspergillus fumigatus-specific Taqman real-time PCR assay for the diagnosis of invasive aspergillosis (IA) with the enzyme-linked immunosorbent assay Platelia Aspergillus method. Patients with proven or probable IA had positive results with at least one of the two methods. The PCR method seems to be more specific, and a combination of the two should improve the diagnosis of IA.
authors.affiliation: Laboratoire de Microbiologie, Hôpital Necker-Enfants Malades, Paris, France. svetlana.challier@nck.ap-hop.paris.fr
authors.forename: Svetlana
authors.initials: S
authors.lastname: Challier
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Boyer
authors.forename: Eric
authors.initials: E
authors.lastname: Abachin
authors.forename: Patrick
authors.initials: P
authors.lastname: Berche
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001228:Aspergillosis / Q000175:diagnosis*; D001232:Aspergillus fumigatus / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D017931:DNA Primers