title: Plasma transport of vitamin K in men using deuterium-labeled collard greens.
abstract: The plasma transport of stable isotope-labeled phylloquinone at physiologic doses from food was studied. A single bolus of 100 g (396 +/- 28 microg phylloquinone) deuterium-labeled collard greens was fed with a breakfast containing 24 g fat to 5 men (26 to 71 years). Eleven blood samples were obtained over 216 hours. Phylloquinone concentrations in plasma and lipoprotein subfractions were measured using high-performance liquid chromatography (HPLC), and the ion abundances of deuterated and endogenous phylloquinone were determined using gas chromatography/mass spectrometry (GC/MS). Plasma total phylloquinone concentrations peaked at 6 to 9 hours (10.51 +/- 4.38 to 8.30 +/- 4.64 nmol/L) and returned to baseline by 24 hours (1.26 +/- 0.38 nmol/L). The triglyceride-rich lipoprotein (TRL) fraction was the major carrier of phylloquinone; low-density lipoprotein (LDL) and high-density lipoprotein (HDL) fractions contained smaller amounts. Maximum enrichment of plasma and TRL phylloquinone with deuterium (88% and 89%, respectively) was reached at 6 hours, respectively; t(1/2) was 22.8 hours (n = 3). Deuterated-phylloquinone was not detectable in plasma or TRL fraction at 72 hours. These results suggest rapid uptake and transport of physiologic doses of phylloquinone.
authors.affiliation: Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA, USA.
authors.forename: Arja T
authors.initials: AT
authors.lastname: Erkkilä
authors.forename: Alice H
authors.initials: AH
authors.lastname: Lichtenstein
authors.forename: Gregory G
authors.initials: GG
authors.lastname: Dolnikowski
authors.forename: Michael A
authors.initials: MA
authors.lastname: Grusak
authors.forename: Susan M
authors.initials: SM
authors.lastname: Jalbert
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Aquino
authors.forename: James W
authors.initials: JW
authors.lastname: Peterson
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Booth
mesh_terms: D000328:Adult; D000368:Aged; D001937:Brassica / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D003903:Deuterium; D006801:Humans; D008055:Lipids / Q000097:blood; D008074:Lipoproteins / Q000097:blood; D008297:Male; D008875:Middle Aged; D014280:Triglycerides / Q000097:blood; D014812:Vitamin K / Q000097:blood*
chemical_list: D008055:Lipids; D008074:Lipoproteins; D014280:Triglycerides; D014812:Vitamin K; D002784:Cholesterol; D003903:Deuterium