title: Molecular characterization of Porcine circovirus type 2 isolates from post-weaning multisystemic wasting syndrome-affected and non-affected pigs.
abstract: Porcine circovirus type 2 (PCV2) is recognized as a primary cause in post-weaning multisystemic wasting syndrome (PMWS). In this study, both PCV1 and PCV2 types were studied in pigs originating from PMWS-affected (+) and non-affected (-) herds from Brittany. PCV2 was identified by PCR in 100 % of animals from PMWS(+) herds and in 76 % from PMWS(-) herds, while PCV1 was not detected. The complete sequences of 38 PCV2 isolates were determined and 23 new variants were identified, displaying between 94.6 and 99.9 % nucleotide identity with one another. Although highly related to all the PCV2 sequences available in databases, the isolates from France gathered in a distinct subcluster. Compared with the 13 PCV2 from PMWS(+) farms, the 10 PMWS(-) sequences exhibited a slightly higher variability. No viral molecular marker specific to a pathogenic state could be identified, even by including other PCV2 variants isolated from PMWS-suffering animals from other countries. We concluded that the PMWS outbreaks in Brittany are most likely not due to the emergence of a new genotype of circovirus.
authors.affiliation: French Food Safety Agency, Viral Genetics and Biosafety Unit, BP 53, F-22 440 Ploufragan, France.
authors.forename: Claire
authors.initials: C
authors.lastname: de Boisséson
authors.affiliation: French Food Safety Agency, Viral Genetics and Biosafety Unit, BP 53, F-22 440 Ploufragan, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Béven
authors.affiliation: French Food Safety Agency, Viral Genetics and Biosafety Unit, BP 53, F-22 440 Ploufragan, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Bigarré
authors.affiliation: French Food Safety Agency, Fish Infectious Diseases and Parasitology Unit, BP 70, F-29 280 Plouzané, France.
authors.forename: Richard
authors.initials: R
authors.lastname: Thiéry
authors.affiliation: Epidemiology and Quality on Pig Production Unit, BP 53, F-22 440 Ploufragan, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Rose
authors.affiliation: Epidemiology and Quality on Pig Production Unit, BP 53, F-22 440 Ploufragan, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Eveno
authors.affiliation: Epidemiology and Quality on Pig Production Unit, BP 53, F-22 440 Ploufragan, France.
authors.forename: François
authors.initials: F
authors.lastname: Madec
authors.affiliation: French Food Safety Agency, Viral Genetics and Biosafety Unit, BP 53, F-22 440 Ploufragan, France.
authors.forename: André
authors.initials: A
authors.lastname: Jestin
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000453:epidemiology / Q000662:veterinary*; D017925:Circovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics; D005602:France / Q000453:epidemiology; D014644:Genetic Variation; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000821:virology*; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*; D019282:Wasting Syndrome / Q000453:epidemiology / Q000662:veterinary*
chemical_list: D004279:DNA, Viral