title: B lineage-specific regulation of V(D)J recombinase activity is established in common lymphoid progenitors.
abstract: Expression of V(D)J recombinase activity in developing lymphocytes is absolutely required for initiation of V(D)J recombination at antigen receptor loci. However, little is known about when during hematopoietic development the V(D)J recombinase is first active, nor is it known what elements activate the recombinase in multipotent hematopoietic progenitors. Using mice that express a fluorescent transgenic V(D)J recombination reporter, we show that the V(D)J recombinase is active as early as common lymphoid progenitors (CLPs) but not in the upstream progenitors that retain myeloid lineage potential. Evidence of this recombinase activity is detectable in all four progeny lineages (B, T, and NK, and DC), and rag2 levels are the highest in progenitor subsets immediately downstream of the CLP. By single cell PCR, we demonstrate that V(D)J rearrangements are detectable at IgH loci in approximately 5% of splenic natural killer cells. Finally, we show that recombinase activity in CLPs is largely controlled by the Erag enhancer. As activity of the Erag enhancer is restricted to the B cell lineage, this provides the first molecular evidence for establishment of a lineage-specific transcription program in multipotent progenitors.
authors.affiliation: Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Ave. North, Worcester 01655, USA.
authors.forename: Lisa
authors.initials: L
authors.lastname: Borghesi
authors.forename: Lih-Yun
authors.initials: LY
authors.lastname: Hsu
authors.forename: Juli P
authors.initials: JP
authors.lastname: Miller
authors.forename: Michael
authors.initials: M
authors.lastname: Anderson
authors.forename: Leonard
authors.initials: L
authors.lastname: Herzenberg
authors.forename: Leonore
authors.initials: L
authors.lastname: Herzenberg
authors.forename: Mark S
authors.initials: MS
authors.lastname: Schlissel
authors.forename: David
authors.initials: D
authors.lastname: Allman
authors.forename: Rachel M
authors.initials: RM
authors.lastname: Gerstein
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000201:enzymology / Q000276:immunology*; D049452:Green Fluorescent Proteins; D006412:Hematopoietic Stem Cells / Q000201:enzymology / Q000276:immunology*; D018398:Homeodomain Proteins / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics; D038041:Lymphopoiesis / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D016133:Polymerase Chain Reaction; D045567:VDJ Recombinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D018398:Homeodomain Proteins; D008164:Luminescent Proteins; C064658:RAG-1 protein; D049452:Green Fluorescent Proteins; D045567:VDJ Recombinases