[Gene expression of stress activated protein kinase and its MAPKs in hypertrophic scar].
OBJECTIVE
To explore the change of gene expression of stress activated protein kinase (SAPK) and its upstream signal-regulated molecule--mitogen activated protein kinases(MAPKs) (MKK4 and MKK7) in hypertrophic scar and auto-control normal skin.


METHODS
The total RNA was isolated from 8 hypertrophic scars and 8 auto-control skin, and then mRNA was purified. The gene expressions of MKK4, MKK7 and SAPK were examined with reverse transcription-polymerase chain reaction(RT-PCR) method.


RESULTS
In hypertrophic scar, both MKK7 and SAPK genes weakly expressed. In auto-control skin, the expression of these 2 genes was significantly elevated in comparison with hypertrophic scar (P < 0.01). The expression levels of these 2 genes were 1.5 times and 2.6 times as long as those of hypertrophic scar, respectively. Gene expression of MKK4 had no significant difference between auto-control skin and hypertrophic scar (P > 0.05).


CONCLUSIONS
Decreased gene expression of MKK7 and SAPK which results in reducing cell apoptosis might be one of the mechanisms for controlling the formation of hypertrophic scar.
Chen
Wei
W
Key Research Laboratory of Wound Repair, 304th Hospital of PLA, Beijing, P. R. China 100037.
Fu
Xiao-bing
XB
Sun
Tong-zhu
TZ
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D017439:Cicatrix, Hypertrophic / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000201:enzymology / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics*; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D012867:Skin / Q000201:enzymology*
D012333:RNA, Messenger; D020928:Mitogen-Activated Protein Kinases