title: Galectin-1 induces cell adhesion to the extracellular matrix and apoptosis of non-adherent human colon cancer Colo201 cells.
abstract: To isolate cDNAs for molecules involved in cell adhesion to the extracellular matrix, expression cloning with non-adherent colon cancer Colo201 cells was carried out. Four positive clones were isolated and, when sequenced, one was found to be galectin-1, a beta-galactoside-binding protein. When cultured on fibronectin-, laminin-, and collagen-coated and non-coated dishes, the adherent galectin-1 cDNA-transfected Colo201 cells increased and spread somewhat. Immunofluorescence staining revealed that galectin-1 was expressed inside and outside of Colo201 cells. The adhesion was dependent on the carbohydrate-recognition domain of galectin-1 since lactose inhibited the adhesion and exogenously-added galectin-1 caused the adhesion. PD58059, an inhibitor of mitogen-activated protein kinase, or LY294002, a phosphoinositide 3-OH kinase inhibitor, decreased the adhesion. Furthermore, the expression of galectin-1 in Colo201 cells induced apoptotic cell death, while exogenously-added galectin-1 did not cause apoptosis. These results indicate that galectin-1 plays a role in both cell-matrix interactions and the inhibition of Colo201 cell proliferation, and suggest that galectin-1 expressed in cells could be associated with apoptosis.
authors.affiliation: Department of Biotechnology, Kyoto Institute of Technology, Kyoto 606-8585.
authors.forename: Natsuko
authors.initials: N
authors.lastname: Horiguchi
authors.forename: Kei-ichiro
authors.initials: K
authors.lastname: Arimoto
authors.forename: Atsushi
authors.initials: A
authors.lastname: Mizutani
authors.forename: Yoko
authors.initials: Y
authors.lastname: Endo-Ichikawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakada
authors.forename: Shigeru
authors.initials: S
authors.lastname: Taketani
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000302:isolation & purification / Q000502:physiology; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism / Q000502:physiology; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D016326:Extracellular Matrix Proteins / Q000502:physiology*; D037483:Galectin 1 / Q000096:biosynthesis / Q000502:physiology*; D006131:Growth Inhibitors / Q000096:biosynthesis / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis / Q000502:physiology; D014162:Transfection
chemical_list: D015815:Cell Adhesion Molecules; D016326:Extracellular Matrix Proteins; D037483:Galectin 1; D006131:Growth Inhibitors; D008565:Membrane Proteins