Purification, characterization, and gene cloning of cis,cis-muconate cycloisomerase from benzamide-assimilating Arthrobacter sp. BA-5-17.
cis,cis-Muconate cycloisomerase (MC) was purified to homogeneity from benzamide-assimilating Arthrobacter sp. BA-5-17. The purified enzyme showed high activities for cis,cis-muconate and 3-methyl-cis,cis-muconate, and preferred the 3-substituted derivatives over the derivatives with the same substituent at the 2 position as a substrate. A gene encoding MC of strain BA-5-17 was cloned and named catB. The catB gene was clustered with catR encoding a putative LysR-type regulator, catC encoding a putative muconolactone isomerase, and catA-II encoding the catechol 1,2-dioxygenase isozymes CD-III-1 and III-2. These genes showed the same orientation in transcriptional direction and the organization of cloned genes was catRBCA-II. In the phylogenetic analysis of MCs and chloro-MCs, the BA-5-17 and Streptomyces setonii MCs formed a subfamily, clearly distinguished from those of other MCs.
Murakami
Shuichiro
S
Laboratory of Applied Microbiology, Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Nada, Kobe 657-8501, Japan.
Kohsaka
Chihiro
C
Okuno
Takao
T
Takenaka
Shinji
S
Aoki
Kenji
K
D000595:Amino Acid Sequence; D001173:Arthrobacter / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D001549:Benzamides / Q000378:metabolism*; D002396:Catechols / Q000378:metabolism*; D005798:Genes, Bacterial; D005809:Genes, Regulator; D019753:Intramolecular Lyases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny
D001549:Benzamides; D002396:Catechols; C037689:benzamide; D019753:Intramolecular Lyases; C019830:3-carboxy-cis-cis-muconate cycloisomerase