Inhibitory effect of green tea catechins in combination with sucralfate on Helicobacter pylori infection in Mongolian gerbils.
BACKGROUND
The occurrence of antibiotic-resistant Helicobacter pylori has been reported. It is desirable to develop an effective method to prevent the occurrence of resistant strains of Helicobacter pylori. Green tea catechins (GTCs) have been reported to have an antibacterial effect. Therefore, the possibility of eradicating Helicobacter pylori by the oral administration of GTCs was investigated.


METHODS
Solutions of GTCs and solutions of GTCs adsorbed to sucralfate (GTC-scf), at concentrations of 20 mg GTCs and/or 20 mg sucralfate/ml were prepared. Then 1 ml of the GTC-scf or the GTC solution was administered daily, for 10 days to Mongolian gerbils infected with Helicobacter pylori. Then the stomachs were extirpated and homogenized. The homogenate was spread on selective medium plates. After 5-day culture, colony-forming units (CFU) of Helicobacter pylori were counted.


RESULTS
The CFU of Helicobacter pylori was significantly decreased by GTC-scf.


CONCLUSIONS
GTC-scf may have a bactericidal effect on Helicobacter pylori infection.
Takabayashi
Fumiyo
F
Shizuoka College, University of Shizuoka, 2-2-1 Oshika, Shizuoka 422-8021, Japan.
Harada
Noboru
N
Yamada
Masami
M
Murohisa
Binzaburo
B
Oguni
Itaro
I
D000284:Administration, Oral; D000818:Animals; D028241:Camellia sinensis; D002392:Catechin / Q000008:administration & dosage / Q000494:pharmacology*; D005772:Gastrointestinal Transit / Q000187:drug effects*; D005849:Gerbillinae; D016481:Helicobacter Infections / Q000517:prevention & control; D016480:Helicobacter pylori / Q000187:drug effects / Q000302:isolation & purification; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D013392:Sucralfate / Q000008:administration & dosage / Q000494:pharmacology*; D013662:Tea
D010936:Plant Extracts; D013662:Tea; C465546:green tea extract AR25; D013392:Sucralfate; D002392:Catechin