Molecular characterization of Porcine circovirus type 2 isolates from post-weaning multisystemic wasting syndrome-affected and non-affected pigs.
Porcine circovirus type 2 (PCV2) is recognized as a primary cause in post-weaning multisystemic wasting syndrome (PMWS). In this study, both PCV1 and PCV2 types were studied in pigs originating from PMWS-affected (+) and non-affected (-) herds from Brittany. PCV2 was identified by PCR in 100 % of animals from PMWS(+) herds and in 76 % from PMWS(-) herds, while PCV1 was not detected. The complete sequences of 38 PCV2 isolates were determined and 23 new variants were identified, displaying between 94.6 and 99.9 % nucleotide identity with one another. Although highly related to all the PCV2 sequences available in databases, the isolates from France gathered in a distinct subcluster. Compared with the 13 PCV2 from PMWS(+) farms, the 10 PMWS(-) sequences exhibited a slightly higher variability. No viral molecular marker specific to a pathogenic state could be identified, even by including other PCV2 variants isolated from PMWS-suffering animals from other countries. We concluded that the PMWS outbreaks in Brittany are most likely not due to the emergence of a new genotype of circovirus.
de Boisséson
Claire
C
French Food Safety Agency, Viral Genetics and Biosafety Unit, BP 53, F-22 440 Ploufragan, France.
Béven
Véronique
V
French Food Safety Agency, Viral Genetics and Biosafety Unit, BP 53, F-22 440 Ploufragan, France.
Bigarré
Laurent
L
French Food Safety Agency, Viral Genetics and Biosafety Unit, BP 53, F-22 440 Ploufragan, France.
Thiéry
Richard
R
French Food Safety Agency, Fish Infectious Diseases and Parasitology Unit, BP 70, F-29 280 Plouzané, France.
Rose
Nicolas
N
Epidemiology and Quality on Pig Production Unit, BP 53, F-22 440 Ploufragan, France.
Eveno
Eric
E
Epidemiology and Quality on Pig Production Unit, BP 53, F-22 440 Ploufragan, France.
Madec
François
F
Epidemiology and Quality on Pig Production Unit, BP 53, F-22 440 Ploufragan, France.
Jestin
André
A
French Food Safety Agency, Viral Genetics and Biosafety Unit, BP 53, F-22 440 Ploufragan, France.
D000818:Animals; D018173:Circoviridae Infections / Q000453:epidemiology / Q000662:veterinary*; D017925:Circovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics; D005602:France / Q000453:epidemiology; D014644:Genetic Variation; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000821:virology*; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*; D019282:Wasting Syndrome / Q000453:epidemiology / Q000662:veterinary*
D004279:DNA, Viral