title: Approaches for monitoring nuclear translation.
abstract: The nuclear membrane is the defining feature of eukaryotes. It divides the cell into two functionally specialized compartments, and it is widely assumed that translation is restricted to only one: the cytoplasm. However, recent results suggest that some translation takes place in nuclei closely coupled to transcription. Various labeling techniques are described that enable nascent peptides to be labeled and then localized wherever they might be in the cell.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, UK.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Iborra
authors.forename: Dean A
authors.initials: DA
authors.lastname: Jackson
authors.forename: Peter R
authors.initials: PR
authors.lastname: Cook
mesh_terms: D001710:Biotin / Q000378:metabolism; D001896:Boron Compounds; D002455:Cell Division; D002458:Cell Fractionation / Q000379:methods*; D002467:Cell Nucleus / Q000502:physiology*; D003593:Cytoplasm; D005455:Fluorescent Antibody Technique / Q000379:methods; D005456:Fluorescent Dyes; D006367:HeLa Cells; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D008854:Microscopy, Electron; D015530:Nuclear Matrix; D010446:Peptide Fragments / Q000502:physiology*; D014176:Protein Biosynthesis; D012346:RNA, Transfer, Amino Acyl; D012358:RNA, Transfer, Met; D012588:Scintillation Counting
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D010446:Peptide Fragments; D012346:RNA, Transfer, Amino Acyl; D012358:RNA, Transfer, Met; D001710:Biotin