Survival of lactic acid bacteria in simulated gastrointestinal juice protected by a DNA-based complex gel.
Salmon milt DNA was utilized in the approach to protect functional materials, proteins, microorganisms, drugs, etc., by forming a DNA-based complex gel with gelatin and kappa-carrageenan. The multiplier effect of the combination of DNA, gelatin and kappa-carrageenan was examined by measuring the release of methyl orange from the gel after being incubated in simulated gastric juice. The results indicated that the hydrogel made of DNA, gelatin and kappa-carrageenan can efficiently maintain the functional materials. Furthermore, a complex gel was developed and optimized by applying an emulsion of lactic acid bacteria and cacao oil onto the already prepared hydrogel. The existence of the lactic acid bacteria in the oil emulsion particles was confirmed by scanning electron microscopy. The protection capabilities in the simulated gastrointestinal juice, which had a viability of 1.50 x 10(6) CFU/g gel after 2 h incubation in the simulated gastric juice and 2.53 x 10(7) CFU/g gel after 6 h continued incubation in the simulated intestinal juice, were measured, in contrast to 2.58 x 10(7) CPU/g in the original gel before treatment.
Jonganurakkun
Benjamas
B
Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060-08110, Japan.
Liu
Xiang Dong
XD
Nodasaka
Yoshinobu
Y
Nomizu
Motoyoshi
M
Nishi
Norio
N
D002351:Carrageenan / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D003692:Delayed-Action Preparations; D004064:Digestive System / Q000737:chemistry*; D005750:Gastric Juice / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry; D005782:Gels / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007777:Lactobacillaceae / Q000166:cytology* / Q000254:growth & development; D008855:Microscopy, Electron, Scanning; D018716:Molecular Mimicry
D003692:Delayed-Action Preparations; D005782:Gels; D002351:Carrageenan; D005780:Gelatin; D004247:DNA