title: Characterisation of an extracellular serine protease gene (nasp gene) from Dermatophilus congolensis.
abstract: A partial amino acid sequence of a serine protease from Dermatophilus congolensis allowed the design of oligonucleotide primers that were complemented with additional ones from previously published partial sequences of the gene encoding the enzyme. The polymerase chain reaction (PCR), using combinations of specific and degenerate oligonucleotide primers, allowed the amplification of a 1738-bp internal fragment of the gene, which was finally characterised by inverse PCR as the first full-length sequenced serine protease gene (nasp) from Dermatophilus congolensis. The deduced amino acid sequence of this enzyme, probably involved in the pathogenesis of dermatophilosis, links it to the subtilisin family of proteases.
authors.affiliation: Infectious Disease Unit, Medicine and Animal Health, University of Extremadura, Campus UEX, Avda. Universidad s/n, 10071 Caceres, Spain.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Garcia-Sanchez
authors.forename: Rosario
authors.initials: R
authors.lastname: Cerrato
authors.forename: Jose
authors.initials: J
authors.lastname: Larrasa
authors.forename: Nicholas C
authors.initials: NC
authors.lastname: Ambrose
authors.forename: Alberto
authors.initials: A
authors.lastname: Parra
authors.forename: Juan M
authors.initials: JM
authors.lastname: Alonso
authors.forename: Miguel
authors.initials: M
authors.lastname: Hermoso-de-Mendoza
authors.forename: Joaquin M
authors.initials: JM
authors.lastname: Rey
authors.forename: Madisa O
authors.initials: MO
authors.lastname: Mine
authors.forename: Patrick R
authors.initials: PR
authors.lastname: Carnegie
authors.forename: Trevor M
authors.initials: TM
authors.lastname: Ellis
authors.forename: Anne M
authors.initials: AM
authors.lastname: Masters
authors.forename: Alan D
authors.initials: AD
authors.lastname: Pemberton
authors.forename: Javier
authors.initials: J
authors.lastname: Hermoso-de-Mendoza
mesh_terms: D000192:Actinomycetales / Q000737:chemistry / Q000201:enzymology / Q000235:genetics*; D000595:Amino Acid Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000302:isolation & purification; D005784:Gene Amplification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000235:genetics* / Q000276:immunology
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D012697:Serine Endopeptidases