title: Primer pair p289-p290, designed to detect both noroviruses and sapoviruses by reverse transcription-PCR, also detects rotaviruses by cross-reactivity.
abstract: A primer pair (p289-p290) designed to detect both noroviruses and sapoviruses by reverse transcription-PCR (Jiang et al., J. Virol. Methods 83:145, 1999) cross-reacts with rotaviruses. The rotavirus amplicon corresponds to genome segment 1. Furthermore, primer pair p289-p290 detected rotaviruses as efficiently as rotavirus-specific primers directed to rotavirus gene 4.
authors.affiliation: Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela. jeludert@ivic.ve
authors.forename: Juan E
authors.initials: JE
authors.lastname: Ludert
authors.forename: Ana C
authors.initials: AC
authors.lastname: Alcalá
authors.forename: Ferdinando
authors.initials: F
authors.lastname: Liprandi
mesh_terms: D001483:Base Sequence; D003429:Cross Reactions; D017931:DNA Primers; D008969:Molecular Sequence Data; D029322:Norovirus / Q000145:classification* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012401:Rotavirus / Q000145:classification* / Q000235:genetics; D029341:Sapovirus / Q000145:classification* / Q000235:genetics; D013045:Species Specificity
chemical_list: D017931:DNA Primers