title: Evidence for an intermediate in tau filament formation.
abstract: Alzheimer's disease is defined in part by the intraneuronal accumulation of filaments comprised of the microtubule-associated protein tau. In vitro, fibrillization of full-length, unphosphorylated recombinant tau can be induced under near-physiological conditions by treatment with various agents, including anionic surfactants. Here we examine the pathway through which anionic surfactants promote tau fibrillization using a combination of electron microscopy and fluorescence spectroscopy. Protein and surfactant first interacted in solution to form micelles, which then provided negatively charged surfaces that accumulated tau aggregates. Surface aggregation of tau protein was followed by the time-dependent appearance of a thioflavin S reactive intermediate that accumulated over a period of hours. The intermediate was unstable in the absence of anionic surfaces, suggesting it was not filamentous. Fibrillization proceeded after intermediate formation with classic nucleation-dependent kinetics, consisting of lag phase followed by the exponential increase in filament lengths, followed by an equilibrium phase reached in approximately 24 h. The pathway did not require protein insertion into the micelle hydrophobic core or conformational change arising from mixed micelle formation, because anionic microspheres constructed from impermeable polystyrene were capable of qualitatively reproducing all aspects of the fibrillization reaction. It is proposed that the progression from amorphous aggregation through intermediate formation and fibrillization may underlie the activity of other inducers such as hyperphosphorylation and may be operative in vivo.
authors.affiliation: Biophysics Program, The Ohio State University College of Medicine and Public Health, Columbus, Ohio 43210, USA.
authors.forename: Carmen N
authors.initials: CN
authors.lastname: Chirita
authors.forename: Jeff
authors.initials: J
authors.lastname: Kuret
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism*; D000838:Anions / Q000737:chemistry; D016718:Arachidonic Acid / Q000737:chemistry; D052160:Benzothiazoles; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007381:Intermediate Filament Proteins / Q000737:chemistry / Q000648:ultrastructure; D008823:Micelles; D008863:Microspheres; D016874:Neurofibrillary Tangles / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D016900:Neurofilament Proteins / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D013050:Spectrometry, Fluorescence; D055672:Static Electricity; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry; D016875:tau Proteins / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D000838:Anions; D052160:Benzothiazoles; D007381:Intermediate Filament Proteins; D008823:Micelles; D016900:Neurofilament Proteins; D011994:Recombinant Proteins; D013501:Surface-Active Agents; D013844:Thiazoles; D016875:tau Proteins; C009462:thioflavin T; D016718:Arachidonic Acid