Survey of molecular methods for the typing of wine yeast strains.
A survey of the genetic polymorphisms produced by distinct methods was performed in 23 commercial winery yeast strains. Microsatellite typing, using six different loci, an optimized interdelta sequence analysis and restriction fragment length polymorphism of mitochondrial DNA generated by the enzyme HinfI had the same discriminatory power: among the 23 commercial yeast strains, 21 distinct patterns were obtained. Karyotype analysis gave 22 patterns, thereby allowing the discrimination of one of the three strains that were not distinguished by the other methods. Due to the equivalence of the results obtained in this survey, any of the methods can be applied at the industrial scale.
Schuller
Dorit
D
Departamento de Biologia, Campus de Gualtar, Universidade do Minho, 4710-057 Braga, Portugal.
Valero
Eva
E
Dequin
Sylvie
S
Casal
Margarida
M
D004272:DNA, Mitochondrial / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D005285:Fermentation; D005516:Food Microbiology; D007621:Karyotyping; D018895:Microsatellite Repeats; D016533:Mycological Typing Techniques; D012150:Polymorphism, Restriction Fragment Length; D014920:Wine / Q000382:microbiology*; D015003:Yeasts / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D004272:DNA, Mitochondrial