title: Effect of overexpression of Actinobacillus succinogenes phosphoenolpyruvate carboxykinase on succinate production in Escherichia coli.
abstract: Succinate fermentation was investigated in Escherichia coli strains overexpressing Actinobacillus succinogenes phosphoenolpyruvate carboxykinase (PEPCK). In E. coli K-12, PEPCK overexpression had no effect on succinate fermentation. In contrast, in the phosphoenolpyruvate carboxylase mutant E. coli strain K-12 ppc::kan, PEPCK overexpression increased succinate production 6.5-fold.
authors.affiliation: Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, USA.
authors.forename: Pil
authors.initials: P
authors.lastname: Kim
authors.forename: Maris
authors.initials: M
authors.lastname: Laivenieks
authors.forename: Claire
authors.initials: C
authors.lastname: Vieille
authors.forename: J Gregory
authors.initials: JG
authors.lastname: Zeikus
mesh_terms: D000188:Actinobacillus / Q000201:enzymology* / Q000235:genetics*; D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D005285:Fermentation; D005818:Genetic Engineering / Q000379:methods; D009154:Mutation; D019764:Phosphoenolpyruvate Carboxykinase (ATP) / Q000235:genetics / Q000378:metabolism*; D010730:Phosphoenolpyruvate Carboxylase / Q000235:genetics / Q000378:metabolism; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D003470:Culture Media; D019802:Succinic Acid; D010730:Phosphoenolpyruvate Carboxylase; D019764:Phosphoenolpyruvate Carboxykinase (ATP)