Role of neutrophils on shock/resuscitation-mediated intestinal arteriolar derangements.
Adequate resuscitation from hemorrhagic shock that preserves hemodynamics is associated with a generalized and progressive intestinal arteriolar vasoconstriction and hypoperfusion coupled with impairment of the endothelium-dependent dilation response. This study was performed to investigate the role of neutrophils on the postresuscitation intestinal arteriolar derangements. Experiments were performed in anesthetized rats 24 h after neutrophil depletion. Neutropenia was induced with antineutrophil serum by tail vein injection. Rats injected with rabbit serum lacking anti-rat neutrophil antibody served as controls. Hemorrhagic shock was 50% of mean arterial pressure for 60 min. Resuscitation was with the shed blood returned plus 2 volumes of saline. A nonhemorrhage group served as control. Intravital videomicroscopy of the terminal ileum was used to measure microvascular diameter and centerline red cell velocity. Endothelial function was assessed from the response to the endothelium-dependent dilator acetylcholine (10(-9) to 10(-4) M). Regardless of neutrophil count, hemorrhagic shock caused selective vasoconstriction of inflow A1 arterioles (-21.49 +/- 0.67%) from baseline, which was not seen in the premucosal A3 vessels (pA3, dA3). At 2 h postresuscitation, there was a generalized vasoconstriction from baseline diameter in A1 (-21.26 +/- 2.29%), pA3 (-22.66 +/- 5.02%), and dA3 (-17.62 +/- 4.84%). Neutrophil depletion caused a significant reset of baseline A1 blood flow from 701 +/- 90 nL/s to 978 +/- 90 nL/s and attenuated the postresuscitation hypoperfusion. This occurred independently of the A1 diameter change. Hemorrhagic shock/resuscitation caused impairment of the endothelium-dependent dilation response irrespective of neutrophil count. This study demonstrates that neutrophils do not contribute to the hemorrhagic/resuscitation-mediated intestinal arteriolar derangements, but appear to possess a role in the intestinal arteriolar blood flow regulation under normal and low flow states possibly via a rheologic effect.
Zakaria
El Rasheid
el R
Department of Physiology and Biophysics, University of Louisville, Louisville, Kentucky 40292, USA. drelzak@aol.com
Garrison
R Neal
RN
Kawabe
Touichi
T
Harris
Patrick D
PD
D000109:Acetylcholine / Q000737:chemistry; D000818:Animals; D001160:Arterioles / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004727:Endothelium / Q000166:cytology; D004912:Erythrocytes; D007082:Ileum / Q000378:metabolism; D007422:Intestines / Q000098:blood supply*; D008297:Male; D008833:Microcirculation; D009504:Neutrophils / Q000378:metabolism / Q000502:physiology*; D016877:Oxidants / Q000378:metabolism; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow; D012044:Regression Analysis; D012151:Resuscitation; D012771:Shock, Hemorrhagic / Q000097:blood* / Q000378:metabolism; D013997:Time Factors
D016877:Oxidants; D006152:Cyclic GMP; D000109:Acetylcholine