Identification of Bordetella pertussis in a critically ill human immunodeficiency virus-infected patient by direct genotypical analysis of Gram-stained material and discrimination from B. holmesii by using a unique recA gene restriction enzyme site.
Bordetella pertussis was diagnosed in a human immunodeficiency virus-infected patient by a newly developed method in which bacterial DNA is amplified directly from sputum Gram-stained slides. The validation of the method is described along with an additional new PCR-based assay that can distinguish between B. pertussis and Bordetella holmesii.
Vielemeyer
Ole
O
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06520-8035, USA.
Crouch
Jill Y
JY
Edberg
Stephen C
SC
Howe
John G
JG
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis*; D001886:Bordetella pertussis / Q000235:genetics* / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000235:genetics; D005838:Genotype; D015658:HIV Infections; D006801:Humans; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics*; D015183:Restriction Mapping / Q000379:methods; D013183:Sputum / Q000382:microbiology; D014917:Whooping Cough / Q000175:diagnosis*
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D011938:Rec A Recombinases