Proteomic and immunochemical characterization of a role for stathmin in adult neurogenesis.
Stathmin is a developmentally regulated cytosolic protein expressed at high levels in the brain. Two-dimensional differential in-gel electrophoresis and mass spectroscopy of proteins expressed in immature and mature cultures from embryonic rat cerebral cortex identified stathmin among several differentially expressed proteins, consistent with a possible role in neurogenesis. Stathmin immunohistochemistry in adult rodent brain revealed prominent expression in neuroproliferative zones and neuronal migration pathways, a pattern that resembles the expression of doublecortin, which is implicated in neuronal migration. Stathmin immunoreactivity was also associated with neurons undergoing ectopic chain migration into the ischemic striatum and cerebral cortex following focal cerebral ischemia. Reducing the expression of stathmin or doublecortin with an antisense oligonucleotide inhibited the migration of new neurons from the subventricular zone to the olfactory bulb via the rostral migratory stream. These results suggest a role for stathmin in the migration of newborn neurons in the adult brain.
Jin
Kunlin
K
Buck Institute for Age Research, Novato, California 94945, USA.
Mao
Xiao Ou
XO
Cottrell
Barbara
B
Schilling
Birgit
B
Xie
Lin
L
Row
Richard H
RH
Sun
Yunjuan
Y
Peel
Alyson
A
Childs
Jocelyn
J
Gendeh
Gurmil
G
Gibson
Bradford W
BW
Greenberg
David A
DA
D000375:Aging / Q000502:physiology*; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002545:Brain Ischemia; D002455:Cell Division; D002465:Cell Movement; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology / Q000293:injuries / Q000378:metabolism*; D000089662:Doublecortin Protein; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D008868:Microtubule Proteins; D009024:Morphogenesis; D009474:Neurons / Q000378:metabolism; D009836:Oligodendroglia / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D050777:Stathmin; D014357:Trypsin / Q000378:metabolism
D015415:Biomarkers; C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; D008868:Microtubule Proteins; D010750:Phosphoproteins; D050777:Stathmin; D014357:Trypsin