title: Synthesis of (2S,4S)- and (2S,4R)-5-fluoroleucine and (2S,4S)-[5,5-2H2]-5-fluoroleucine.
abstract: Syntheses of (2S,4S)- and (2S,4R)-5-fluoroleucine, and, and of (2S,4S)-[5,5-(2)H(2)]-5-fluoroleucine, have been completed. The methodology allows these compounds to be prepared in sufficient quantities for incorporation by solid-state protein synthesis into strategic sites in proteins for folding studies. X-ray structures of the epimers and have been obtained and show the presence of conformational isomerism. The torsion angles between the F-C bond and the main chain are compared with values found in a mutant of the protein ubiquitin in which (2S,4S)-5-fluoroleucine replaces leucine residues 50 and 67 in the native protein.
authors.affiliation: Sussex Centre for Biomolecular Design and Drug Discovery, Department of Chemistry, University of Sussex, Falmer, Brighton, UK.
authors.forename: Jean-Damien
authors.initials: JD
authors.lastname: Charrier
authors.forename: David S
authors.initials: DS
authors.lastname: Hadfield
authors.forename: Peter B
authors.initials: PB
authors.lastname: Hitchcock
authors.forename: Douglas W
authors.initials: DW
authors.lastname: Young