[The inhibitory effect of 8-chloroadenosine on tumor necrosis factor-induced proto-oncogene expression of retinal pigment epithelial cells].
OBJECTIVE
To investigate the inhibitory effect of 8-chloroadenodine (8-CLA) on tumor necrosis factor-alpha (TNF-alpha)-induced proto-oncogene expression in retinal pigment epithelial (RPE) cells.


METHODS
Primary culture and subculture of health bovine RPE cells were established in vitro. RPE cells were treated with tumor necrosis factor TNF-alpha (13.3 mmol/L) with or without 8-CLA (16 micro mol/L) for 30 minutes or 3 hours, respectively. C-fos and c-myc mRNA expression in RPE cells was detected by Northern blot analysis.


RESULTS
Normal RPE cells expressed c-fos and c-myc mRNA at a low level. After treatment with TNF-alpha, the expression of c-fos and c-myc mRNA was increased by 3.13 and 1.5 fold, respectively, as compared to untreated cells. 8-CLA decreased TNF-alpha-induced c-fos and c-myc mRNA expression by 25.0% and 29.0%, respectively.


CONCLUSIONS
8-CLA can inhibit TNF-alpha-induced c-fos and c-myc mRNA expression in RPE cells. These findings may be useful for exploring new drugs for inhibiting the growth-induced proliferation of RPE.
Yang
Ping
P
The Third Hospital of Peking University, Peking University Eye Center, Beijing 100083, China.
Zhang
Hui-rong
HR
D015762:2-Chloroadenosine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D016762:Genes, fos; D016259:Genes, myc; D007150:Immunohistochemistry; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D015762:2-Chloroadenosine; D012313:RNA; C075440:8-chloroadenosine