Novel method using a laser scanning cytometer for detection of mycobacteria in clinical samples.
In order to evaluate the capacity of laser scanning cytometry (LSC) to detect acid-fast bacilli directly on clinical samples, a comparison between Kinyoun-stained smears analyzed under light microscopy and propidium iodide-auramine-stained smears analyzed by LSC was performed. The results were compared with those for culture on BACTEC MGIT 960. LSC is a new, reliable methodology to detect MYCOBACTERIA:
Pina-Vaz
Cidália
C
Department of Microbiology, Faculty of Medicine, University of Porto, Hospital S João, Porto, Portugal. micteam@clix.pt
Costa-Oliveira
Sofia
S
Rodrigues
Acácio Gonçalves
AG
Salvador
Alexandre
A
D003592:Cytophotometry / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D018613:Microscopy, Confocal / Q000379:methods; D009161:Mycobacterium / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000175:diagnosis*; D013048:Specimen Handling / Q000379:methods
D007202:Indicators and Reagents