RNA unwinding assay for DExD/H-box RNA helicases.
The evolutionarily conserved DExD/H-box proteins are essential for all RNA-related biological processes. They are thought to modulate the structure and function of specific RNAs and/or ribonucleoprotein particles by using their intrinsic RNA-dependent ATPase activities to achieve the desired conformational changes. A number of DExD/H-box proteins have been shown to unwind short RNA duplexes in vitro, a hallmark of the so-called RNA helicases or unwindases. However, some are unable to do so, perhaps because of requirements for cofactors. Here, we present a "solid-state" method that may allow investigators to overcome such problems.
Tseng-Rogenski
Stephanie S
SS
Department of Molecular Genetics, The Ohio State University, Columbus, OH, USA.
Chang
Tien-Hsien
TH
D001483:Base Sequence; D053487:DEAD-box RNA Helicases; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D029543:Nucleocytoplasmic Transport Proteins / Q000235:genetics* / Q000378:metabolism; D011233:Precipitin Tests; D012313:RNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020365:RNA Helicases / Q000235:genetics* / Q000378:metabolism; D012326:RNA Splicing
D029543:Nucleocytoplasmic Transport Proteins; D012313:RNA; C121148:DDX19B protein, human; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases