[Stimulation of insulin-like growth factor-I to chondrogenesis of engineering cartilage tissue].
OBJECTIVE
To explore the ability of insulin-like growth factor-I (IGF-I) and hyaluracan acid in prompting chondrogenesis of engineering cartilage tissue.


METHODS
Human articular chondrocytes were isolated and cultured in DMEM plus 10% fetal bovine serum. They were divided into three groups: hyaluracan acid + chondrocytes + IGF-I group (IGF-I group), hyaluracan acid + chondrocytes group (cell group), hyaluracan acid group (control group). The ability of chondrogenesis was investigated by HE and toluidine blue staining, human collagen II immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR).


RESULTS
Both cell group and IGF-I group could develop into cartilage tissue in the sixth week while control group could not. The number of cartilage lacuna in IGF-I group were more than that in cell group. Human collagen II immunohistochemistry showed that there were stronger positive cell in IGF-I group than in cell group, collagen II mRNA expression was more higher and collagen I mRNA expression was lower in IGF-I group than in cell group.


CONCLUSIONS
Insulin growth factor-I can prompt chondrogenesis of engineering cartilage tissue and ameliorate the quality of engineering cartilage tissue in vitro.
Huang
Jian-rong
JR
Department of Orthopedics, Second Affiliated Hospital of Sun Yet-Sen University, Guangzhou, Guangdong, P. R. China 510120.
Liu
Shang-li
SL
Song
Wei-dong
WD
D002358:Cartilage, Articular / Q000166:cytology* / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000502:physiology; D020219:Chondrogenesis / Q000187:drug effects*; D024043:Collagen Type II / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D011108:Polymers / Q000378:metabolism; D023822:Tissue Engineering
D024043:Collagen Type II; D011108:Polymers; D007334:Insulin-Like Growth Factor I