title: Hepatic dendritic cell subsets in the mouse.
abstract: The CD11c(+) cell population in the non-parenchymal cell population of the mouse liver contains dendritic cells (DC), NK cells, B cells and T cells. In the hepatic CD11c(+) DC population from immunocompetent or immunodeficient [recombinase-activating gene-1 (RAG1)(-/-)] C57BL/6 mice (rigorously depleted of T cells, B cells and NK cells), we identified a B220(+) CD11c(int) subset of 'plasmacytoid' DC, and a B220(-) CD11c(+) DC subset. The latter DC population could be subdivided into a major, immature (CD40(lo) CD80(lo) CD86(lo) MHC class II(lo)) CD11c(int) subset, and a minor, mature (CD40(hi) CD80(hi) CD86(hi) MHC class II(hi)) CD11c(hi) subset. Stimulated B220(+) but not B220(-) DC produced type I interferon. NKT cell activation in vivo increased the number of liver B220(-) DC three- to fourfold within 18 h post-injection, and up-regulated their surface expression of activation marker, while it contracted the B220(+) DC population. Early in virus infection, the hepatic B220(+) DC subset expanded, and both, the B220(+) as well as B220(-) DC populations in the liver matured. In vitro, B220(-) but not B220(+) DC primed CD4(+) or CD8(+)T cells. Expression of distinct marker profiles and functions, and distinct early reaction to activation signals hence identify two distinct B220(+) and B220(-) subsets in CD11c(+) DC populations freshly isolated from the mouse liver.
authors.affiliation: Department of Medical Microbiology, University of Ulm, Ulm, Germany.
authors.forename: Ieva
authors.initials: I
authors.lastname: Jomantaite
authors.forename: Nektarios
authors.initials: N
authors.lastname: Dikopoulos
authors.forename: Andrea
authors.initials: A
authors.lastname: Kröger
authors.forename: Frank
authors.initials: F
authors.lastname: Leithäuser
authors.forename: Hansjörg
authors.initials: H
authors.lastname: Hauser
authors.forename: Reinhold
authors.initials: R
authors.lastname: Schirmbeck
authors.forename: Jörg
authors.initials: J
authors.lastname: Reimann
mesh_terms: D000818:Animals; D039521:CD11c Antigen / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005434:Flow Cytometry; D005699:Galactosylceramides / Q000276:immunology; D022781:Hepatocytes / Q000166:cytology / Q000276:immunology; D016130:Immunophenotyping; D007370:Interferon Type I / Q000276:immunology / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000276:immunology; D008099:Liver / Q000166:cytology / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D018146:Muromegalovirus / Q000276:immunology
chemical_list: D039521:CD11c Antigen; D005699:Galactosylceramides; D007370:Interferon Type I; D017493:Leukocyte Common Antigens