Biochemical characterization of a Rhizobium etli monovalent cation-stimulated acyl-coenzyme A carboxylase with a high substrate specificity constant for propionyl-coenzyme A.
Biotin has a profound effect on the metabolism of rhizobia. It is reported here that the activities of the biotin-dependent enzymes acetyl-coenzyme A carboxylase (ACC; EC 6.4.1.2) and propionyl-coenzyme A carboxylase (PCC; EC 6.4.1.3) are present in all species of the five genera comprising the Rhizobiaceae which were examined. Evidence is presented that the ACC and PCC activities detectable in Rhizobium etli extracts are catalysed by a single acyl-coenzyme A carboxylase. The enzyme from R. etli strain 12-53 was purified 478-fold and displayed its highest activity with propionyl-CoA as substrate, with apparent K(m) and V(max) values of 0.064 mM and 2885 nmol min(-1) (mg protein)(-1), respectively. The enzyme carboxylated acetyl-CoA and butyryl-CoA with apparent K(m) values of 0.392 and 0.144 mM, respectively, and V(max) values of 423 and 268 nmol min(-1) (mg protein)(-1), respectively. K(+), or Cs(+) markedly activated the enzyme, which was essentially inactive in their absence. Electrophoretic analysis indicated that the acyl-CoA carboxylase was composed of a 74 kDa biotin-containing alpha subunit and a 45 kDa biotin-free beta subunit, and gel chromatography indicated a total molecular mass of 620 000 Da. The strong kinetic preference of the enzyme for propionyl-CoA is consistent with its participation in an anaplerotic pathway utilizing this substrate.
Dunn
Michael F
MF
Programa de Ingeniería Metabólica, Centro de Investigación sobre Fijación de Nitrógeno, Universidad National Autónoma de México, A. P. 565-A, Cuernavaca, Morelos, Mexico.
Araíza
Gisela
G
Programa de Ingeniería Metabólica, Centro de Investigación sobre Fijación de Nitrógeno, Universidad National Autónoma de México, A. P. 565-A, Cuernavaca, Morelos, Mexico.
Mora
Jaime
J
Programa de Ingeniería Metabólica, Centro de Investigación sobre Fijación de Nitrógeno, Universidad National Autónoma de México, A. P. 565-A, Cuernavaca, Morelos, Mexico.
D000214:Acyl Coenzyme A / Q000378:metabolism*; D019735:Carbon-Carbon Ligases / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D043586:Methylmalonyl-CoA Decarboxylase / Q000235:genetics / Q000378:metabolism*; D042163:Rhizobium etli / Q000201:enzymology* / Q000235:genetics*; D013045:Species Specificity; D013379:Substrate Specificity
D000214:Acyl Coenzyme A; C009061:propionyl-coenzyme A; D019735:Carbon-Carbon Ligases; C018757:acyl-CoA carboxylase; D043586:Methylmalonyl-CoA Decarboxylase