title: In situ hybridization for detection of nocardial 16S rRNA: reactivity within intracellular inclusions in experimentally infected cynomolgus monkeys--and in Lewy body-containing human brain specimens.
abstract: Our previous studies found that experimental infection of BALB/c mice with the Gram-positive bacterium Nocardia asteroides induced a parkinsonian-type syndrome with levodopa-responsive movement abnormalities, loss of nigrostriatal dopaminergic neurons, depletion of striatal dopamine, and intraneuronal inclusions in the substantia nigra (SN) with an appearance similar to Lewy bodies. In the present study, an in situ hybridization technique was developed to detect nocardial 16S ribosomal RNA (rRNA), using a Nocardia-specific probe (B77). Cerebral cortical specimens from cynomolgus monkeys were examined for the presence of nocardial RNA 48 h, 3.5 months, and 1 year after experimental infection with N. asteroides. Hybridization reactions were detected within Nocardia-like structures 48 h after infection and within intracellular inclusion bodies (immunoreactive for alpha-synuclein and ubiquitin) in one of two 3.5-month-infected monkeys. The in situ hybridization procedure was then applied in a blinded fashion to 24 human SN specimens with Lewy bodies and 11 human SN specimens without Lewy bodies (including five normal controls). Hybridization reactions were detected in nine Lewy body-containing specimens and none of the others. Reactivity was limited to inclusions with the appearance of Lewy bodies, with the exception of one specimen in which intracellular reactivity was also observed in Nocardia-like structures. These results suggest a possible association between Nocardia and neurodegenerative disorders in which Lewy bodies are present.
authors.affiliation: Department of Medical Microbiology and Immunology, University of California School of Medicine, Davis, CA 95616, USA.
authors.forename: Gail
authors.initials: G
authors.lastname: Chapman
authors.forename: Blaine L
authors.initials: BL
authors.lastname: Beaman
authors.forename: David A
authors.initials: DA
authors.lastname: Loeffler
authors.forename: Dianne M
authors.initials: DM
authors.lastname: Camp
authors.forename: Edward F
authors.initials: EF
authors.lastname: Domino
authors.forename: Dennis W
authors.initials: DW
authors.lastname: Dickson
authors.forename: William G
authors.initials: WG
authors.lastname: Ellis
authors.forename: Ibsen
authors.initials: I
authors.lastname: Chen
authors.forename: Susan E
authors.initials: SE
authors.lastname: Bachus
authors.forename: Peter A
authors.initials: PA
authors.lastname: LeWitt
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000382:microbiology; D000818:Animals; D001921:Brain / Q000382:microbiology* / Q000473:pathology / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D002479:Inclusion Bodies / Q000382:microbiology*; D016631:Lewy Bodies / Q000382:microbiology* / Q000473:pathology; D020961:Lewy Body Disease / Q000382:microbiology; D008252:Macaca fascicularis; D008297:Male; D008875:Middle Aged; D009617:Nocardia Infections / Q000473:pathology*; D009616:Nocardia asteroides / Q000302:isolation & purification; D018075:RNA, Complementary; D012336:RNA, Ribosomal, 16S / Q000302:isolation & purification*
chemical_list: D018075:RNA, Complementary; D012336:RNA, Ribosomal, 16S