Restriction endonuclease-catalyzed cleavage of DNA stimulates subsequent polymerase chain reaction and enables discrimination between normal and mutant K-RAS alleles.
D000483:Alleles; D015179:Colorectal Neoplasms / Q000235:genetics*; D017931:DNA Primers; D004262:DNA Restriction Enzymes / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D004262:DNA Restriction Enzymes; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific