Using codon optimization, chaperone co-expression, and rational mutagenesis for production and NMR assignments of human eIF2 alpha.
D000595:Amino Acid Sequence; D003062:Codon / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015852:Eukaryotic Initiation Factor-2 / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D018832:Molecular Chaperones / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D012995:Solubility
D003062:Codon; D015852:Eukaryotic Initiation Factor-2; D018832:Molecular Chaperones