Monitoring the polysaccharide quality of Agaricus blazei in submerged culture by examining molecular weight distribution and TNF-alpha release capability of macrophage cell line RAW 264.7.
D000364:Agaricus / Q000254:growth & development / Q000378:metabolism*; D000818:Animals; D001681:Biological Assay / Q000379:methods*; D019149:Bioreactors / Q000382:microbiology; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D011134:Polysaccharides / Q000032:analysis* / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*; D011786:Quality Control; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha