title: In vitro mutagenesis to define functional domains.
mesh_terms: D003470:Culture Media / Q000494:pharmacology; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics; D005821:Genetic Techniques; D066298:In Vitro Techniques; D016296:Mutagenesis; D016297:Mutagenesis, Site-Directed; D009841:Oligonucleotides / Q000494:pharmacology; D010766:Phosphorylation; D010957:Plasmids / Q000378:metabolism; D017434:Protein Structure, Tertiary
chemical_list: D003470:Culture Media; D009841:Oligonucleotides