Chimeragenesis of the fatty acid binding site of cytochrome P450BM3. Replacement of residues 73-84 with the homologous residues from the insect cytochrome P450 CYP4C7.
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites / Q000235:genetics; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002384:Catalysis; D003058:Cockroaches; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D000072470:Cytochrome P450 Family 4; D005204:Farnesol / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D006900:Hydroxylation; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009251:NADPH-Ferrihemoprotein Reductase; D010084:Oxidation-Reduction; D019308:Palmitic Acid / Q000378:metabolism; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013237:Stereoisomerism; D013379:Substrate Specificity / Q000235:genetics
D001426:Bacterial Proteins; D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D019476:Insect Proteins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D019308:Palmitic Acid; D005204:Farnesol; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; C115458:CYP4C7 protein, Diploptera punctata; D000072470:Cytochrome P450 Family 4; D009251:NADPH-Ferrihemoprotein Reductase; C052423:flavocytochrome P450 BM3 monoxygenases