In vitro elucidation of substrate specificity and bioassay of proprotein convertase 4 using intramolecularly quenched fluorogenic peptides.
D000818:Animals; D001681:Biological Assay / Q000379:methods; D003374:Coumarins / Q000378:metabolism; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000378:metabolism*; D006801:Humans; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D010455:Peptides / Q000138:chemical synthesis / Q000378:metabolism*; D043484:Proprotein Convertases; D011994:Recombinant Proteins / Q000378:metabolism; D012697:Serine Endopeptidases / Q000172:deficiency / Q000378:metabolism*; D013002:Somatomedins / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013379:Substrate Specificity / Q000502:physiology; D013381:Subtilisins
D003374:Coumarins; D005456:Fluorescent Dyes; D010455:Peptides; D011994:Recombinant Proteins; D013002:Somatomedins; C489716:PCSK4 protein, human; C489717:Pcsk4 protein, mouse; D043484:Proprotein Convertases; D012697:Serine Endopeptidases; D013381:Subtilisins