title: CMP-N-acetylneuraminic acid synthetase from Escherichia coli K1 is a bifunctional enzyme: identification of minimal catalytic domain for synthetase activity and novel functional domain for platelet-activating factor acetylhydrolase activity.
mesh_terms: D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000378:metabolism*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001812:Blood-Brain Barrier; D001921:Brain / Q000201:enzymology; D020134:Catalytic Domain; D002417:Cattle; D002855:Chromatography, Thin Layer; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004305:Dose-Response Relationship, Drug; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D017353:Gene Deletion; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D000216:N-Acylneuraminate Cytidylyltransferase / Q000502:physiology*; D010957:Plasmids / Q000378:metabolism; D011487:Protein Conformation; D020033:Protein Isoforms; D017434:Protein Structure, Tertiary; D012694:Serine / Q000737:chemistry; D012984:Software; D013379:Substrate Specificity; D013696:Temperature; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology
chemical_list: D020033:Protein Isoforms; D012694:Serine; D000216:N-Acylneuraminate Cytidylyltransferase; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D014357:Trypsin