A single-chain Fv intrabody provides functional protection against the effects of mutant protein in an organotypic slice culture model of Huntington's disease.
D000818:Animals; D000831:Animals, Newborn; D019470:Biolistics / Q000379:methods; D002449:Cell Aggregation / Q000187:drug effects; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects; D003342:Corpus Striatum / Q000378:metabolism; D046508:Culture Techniques; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D049452:Green Fluorescent Proteins; D000071058:Huntingtin Protein; D006816:Huntington Disease / Q000139:chemically induced / Q000235:genetics / Q000628:therapy*; D007135:Immunoglobulin Variable Region / Q000276:immunology / Q000627:therapeutic use*; D008164:Luminescent Proteins / Q000378:metabolism; D008222:Lymphokines / Q000627:therapeutic use*; D008314:Malonates / Q000633:toxicity; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010455:Peptides; D011419:Propidium / Q000378:metabolism; D012795:Sialoglycoproteins / Q000627:therapeutic use*; D013997:Time Factors; D014162:Transfection
C089422:Fv protein, human; C506812:Htt protein, mouse; D000071058:Huntingtin Protein; D007135:Immunoglobulin Variable Region; D008164:Luminescent Proteins; D008222:Lymphokines; D008314:Malonates; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D010455:Peptides; D012795:Sialoglycoproteins; D049452:Green Fluorescent Proteins; C097188:polyglutamine; D011419:Propidium; C030290:malonic acid