An engineered PrPsc-like molecule from the chimera of mammalian prion protein and yeast Ure2p prion-inducing domain.
D000682:Amyloid / Q000737:chemistry; D000818:Animals; D052160:Benzothiazoles; D002417:Cattle; D002942:Circular Dichroism; D004396:Coloring Agents / Q000494:pharmacology; D003224:Congo Red / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D019286:Endopeptidase K / Q000737:chemistry; D008853:Microscopy; D008854:Microscopy, Electron; D010957:Plasmids / Q000378:metabolism; D018620:PrPSc Proteins / Q000737:chemistry* / Q000378:metabolism; D011328:Prions / Q000737:chemistry*; D011487:Protein Conformation; D015202:Protein Engineering / Q000379:methods*; D020033:Protein Isoforms; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013844:Thiazoles / Q000737:chemistry
D000682:Amyloid; D052160:Benzothiazoles; D004396:Coloring Agents; D018620:PrPSc Proteins; D011328:Prions; D020033:Protein Isoforms; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D013844:Thiazoles; C009462:thioflavin T; D003224:Congo Red; D019286:Endopeptidase K