title: Refolding and characterization of recombinant human GST-PD-1 fusion protein expressed in Escherichia coli.
mesh_terms: D000818:Animals; D015703:Antigens, CD; D000954:Antigens, Surface / Q000737:chemistry*; D051017:Apoptosis Regulatory Proteins; D001681:Biological Assay; D015153:Blotting, Western; D018106:CD28 Antigens / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D016207:Cytokines / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D005822:Genetic Vectors; D005982:Glutathione Transferase / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D008024:Ligands; D051379:Mice; D010835:Phytohemagglutinins / Q000494:pharmacology; D010957:Plasmids / Q000378:metabolism; D061026:Programmed Cell Death 1 Receptor; D017510:Protein Folding; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism; D013997:Time Factors; D014162:Transfection
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; D051017:Apoptosis Regulatory Proteins; D018106:CD28 Antigens; D016207:Cytokines; D018076:DNA, Complementary; D007376:Interleukin-2; D008024:Ligands; C105992:PDCD1 protein, human; C491383:Pdcd1 protein, mouse; D010835:Phytohemagglutinins; D061026:Programmed Cell Death 1 Receptor; D011993:Recombinant Fusion Proteins; D007371:Interferon-gamma; D005982:Glutathione Transferase