Hyperexpression and purification of biologically active human luteinizing hormone and human chorionic gonadotropin using the methylotropic yeast, Pichia pastoris.
D000818:Animals; D001681:Biological Assay; D002241:Carbohydrates / Q000032:analysis; D006063:Chorionic Gonadotropin / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D005285:Fermentation; D006031:Glycosylation; D006801:Humans; D007985:Leydig Cells / Q000378:metabolism; D007986:Luteinizing Hormone / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008297:Male; D051379:Mice; D010843:Pichia / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011863:Radioimmunoassay; D011869:Radioligand Assay; D051381:Rats; D011974:Receptors, LH / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D002241:Carbohydrates; D006063:Chorionic Gonadotropin; D017931:DNA Primers; D011974:Receptors, LH; D011994:Recombinant Proteins; D007986:Luteinizing Hormone