title: Overexpression and purification of untagged polynucleotide phosphorylases.
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D010710:Phosphates / Q000737:chemistry; D011117:Polyribonucleotide Nucleotidyltransferase / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D017680:Potassium Compounds / Q000737:chemistry; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics; D013303:Streptomyces antibioticus / Q000201:enzymology / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D010710:Phosphates; D017680:Potassium Compounds; D011994:Recombinant Proteins; D000244:Adenosine Diphosphate; C013216:potassium phosphate; D011117:Polyribonucleotide Nucleotidyltransferase