Use of a simple fractionation method to evaluate binding, internalization and intracellular distribution of oligonucleotides in vascular smooth muscle cells.
D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D005591:Chemical Fractionation / Q000379:methods*; D003600:Cytosol / Q000378:metabolism; D004072:Digitonin / Q000737:chemistry / Q000493:pharmacokinetics; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics*; D011094:Polyethyleneimine / Q000737:chemistry / Q000493:pharmacokinetics; D011402:Pronase / Q000378:metabolism; D051381:Rats; D013873:Thionucleotides / Q000378:metabolism / Q000493:pharmacokinetics; D014316:Tritium
D016376:Oligonucleotides, Antisense; D013873:Thionucleotides; D014316:Tritium; D011094:Polyethyleneimine; D011402:Pronase; D004072:Digitonin