Dimethyl sulfoxide at 2.5% (v/v) alters the structural cooperativity and unfolding mechanism of dimeric bacterial NAD+ synthetase.
D000465:Algorithms; D019732:Amide Synthases / Q000737:chemistry*; D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry; D002152:Calorimetry, Differential Scanning; D004121:Dimethyl Sulfoxide / Q000737:chemistry* / Q000494:pharmacology; D004795:Enzyme Stability / Q000187:drug effects; D008956:Models, Chemical; D008958:Models, Molecular; D011487:Protein Conformation / Q000187:drug effects; D011489:Protein Denaturation / Q000187:drug effects; D017510:Protein Folding; D020673:Protein Renaturation; D020836:Protein Structure, Quaternary / Q000187:drug effects; D021122:Protein Subunits / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D013816:Thermodynamics; D014461:Ultracentrifugation
D001426:Bacterial Proteins; D021122:Protein Subunits; D011994:Recombinant Proteins; D019732:Amide Synthases; C038479:NAD+ synthase; D004121:Dimethyl Sulfoxide