Alteration of amino acid residues at the L-chain N-terminus and in complementarity-determining region 3 increases affinity of a recombinant F(ab) for the human N blood group antigen.
D000818:Animals; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000276:immunology*; D016466:CHO Cells; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases / Q000276:immunology*; D022801:Complementarity Determining Regions / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006384:Hemagglutination; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D016296:Mutagenesis / Q000276:immunology; D009363:Neoplasm Proteins / Q000276:immunology*; D017434:Protein Structure, Tertiary; D014162:Transfection
D000951:Antigens, Neoplasm; D022801:Complementarity Determining Regions; D007140:Immunoglobulin Fab Fragments; D009363:Neoplasm Proteins; C089226:CA9 protein, human; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases