A novel strategy to engineer DNA polymerases for enhanced processivity and improved performance in vitro.
D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism; D002021:Buffers; D002384:Catalysis; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000235:genetics* / Q000378:metabolism; D004795:Enzyme Stability; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D011189:Potassium Chloride / Q000737:chemistry; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017384:Sequence Deletion; D019914:Taq Polymerase / Q000235:genetics / Q000378:metabolism
D019843:Archaeal Proteins; D002021:Buffers; D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D011189:Potassium Chloride; D004247:DNA; C072146:Pfu DNA polymerase; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase