The -4 phenylalanine is required for substrate ubiquitination catalyzed by HECT ubiquitin ligases.
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001665:Binding Sites; D002352:Carrier Proteins / Q000378:metabolism; D002384:Catalysis; D017124:Conserved Sequence; D006801:Humans; D010649:Phenylalanine; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D015534:Trans-Activators; D025801:Ubiquitin / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D025801:Ubiquitin; C106908:WBP2 protein, human; D010649:Phenylalanine; D044767:Ubiquitin-Protein Ligases