Overexpression of ovine leptin in Pichia pastoris: physiological yeast response to leptin production and characterization of the recombinant hormone.
D000818:Animals; D019149:Bioreactors; D015139:Blotting, Southern; D015153:Blotting, Western; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D018628:Gene Dosage; D007218:Industrial Microbiology / Q000379:methods*; D020738:Leptin / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D010843:Pichia / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D020539:Sequence Analysis, Protein; D012756:Sheep / Q000235:genetics*; D014170:Transformation, Genetic
D020738:Leptin; D011994:Recombinant Proteins; D004247:DNA