Atopy patch test in patients with atopic eczema/dermatitis syndrome: comparison of petrolatum and aqueous solution as a vehicle.
BACKGROUND
The atopy patch test (APT) is an in vivo model to study the induction of eczema by inhalant allergens. This study was designed to compare two commonly used APT methods.


METHODS
In the first method, the allergen is dissolved in aqueous solution, which is applied on tape-stripped skin. In the second method, the allergen is dissolved in petrolatum and applied without tape stripping. Thirteen patients with atopic dermatitis sensitized to inhalant allergens were patch tested using both methods. Reactions were evaluated macroscopically and microscopically after 48 h.


RESULTS
Nine out of 13 patients displayed a positive reaction for both methods. One patient had a positive APT for the aqueous method alone and three for the petrolatum method alone. Reactions were significantly stronger when using the petrolatum method. Histological evaluation of the nine patients positive for both methods showed no significant differences in number of eosinophils, T-cells and neutrophils.


CONCLUSIONS
The APT using the petrolatum vehicle induces a higher number of positive reactions and is significantly stronger relative to the APT using allergen in aqueous vehicle. The cellular influx in both test methods is comparable. Both methods can be used to study the mechanisms in the induction of eczema by inhalant allergens.
Oldhoff
J M
JM
Department of Dermatology/Allergology, University Medical Centre, Utrecht, The Netherlands.
Bihari
I C
IC
Knol
E F
EF
Bruijnzeel-Koomen
C A F M
CA
de Bruin-Weller
M S
MS
D000328:Adult; D003876:Dermatitis, Atopic / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010328:Patch Tests / Q000379:methods*; D010577:Petrolatum; D014677:Pharmaceutical Vehicles; D012996:Solutions
D014677:Pharmaceutical Vehicles; D012996:Solutions; D010577:Petrolatum