Receptor for advanced glycation end-products is a marker of type I lung alveolar cells.
Lung alveolar epithelial cells are comprised of type I (ATI) and type II (ATII) cells. ATI cells are polarized, although they have very flat morphology. The identification of marker proteins for apical and basolateral membranes of ATI cells is important to investigate into the differentiation of ATI cells. In this paper, we characterized receptor for advanced glycation end-products (RAGE) as a marker for ATI cells. RAGE was localized on basolateral membranes of ATI cells in the immunoelectron microscopy and its expression was enhanced in a parallel manner to the differentiation of ATI cells in vivo and in primary cultures of ATII cells. RAGE and T1 alpha, a well-known ATI marker protein, were targeted to basolateral and apical membranes, respectively, when expressed in polarized Madine Darby canine kidney cells. Moreover, RAGE was expressed in ATI cells after T1 alpha in vivo and in ex in vivo organ cultures. In conclusion, RAGE is a marker for basolateral membranes of well-differentiated ATI cells. ATI cells require some signal provided by the in vivo environment to express RAGE.
Shirasawa
Madoka
M
Department of Medical Biochemistry, Graduate School of Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.
Fujiwara
Naoyuki
N
Hirabayashi
Susumu
S
Ohno
Hideki
H
Iida
Junko
J
Makita
Koshi
K
Hata
Yutaka
Y
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D016764:Cell Polarity; D002478:Cells, Cultured; D004285:Dogs; D005455:Fluorescent Antibody Technique; D006801:Humans; D007668:Kidney / Q000737:chemistry / Q000166:cytology; D008168:Lung / Q000196:embryology; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism; D016253:Microscopy, Immunoelectron; D008858:Microscopy, Phase-Contrast; D009924:Organ Culture Techniques; D011650:Pulmonary Alveoli / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000032:analysis* / Q000378:metabolism; D000098853:Podoplanin
D015415:Biomarkers; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; C117468:PDPN protein, human; D000098853:Podoplanin; C092025:Pdpn protein, rat