The healing effects of Centella extract and asiaticoside on acetic acid induced gastric ulcers in rats.
In this study, the healing effects of Centella asiatica water extract (CE) and asiaticoside (AC), an active constituent of CE, on acetic acid induced gastric ulcers (kissing ulcers) in rats were examined. CE was prepared from Centella asiatica dry plant and the concentration of AC in CE was quantitatively determined with the use of high performance liquid chromatography analysis. Different concentrations of CE and AC were orally administered to rats with kissing ulcers. They were found to reduce the size of the ulcers at day 3 and 7 in a dose-dependent manner, with a concomitant attenuation of myeloperoxidase activity at the ulcer tissues. Epithelial cell proliferation and angiogenesis were on the other hand promoted. The expression of basic fibroblast growth factor, an important angiogenic factor, was also upregulated in the ulcer tissues in rats treated with CE or AC. These results further suggest the potential use of Centella asiatica and its active ingredient as anti-gastric ulcers drugs.
Department of Pharmacology, Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong, China.
Chuen Lung
CL
Cheng
Jin Sheng
JS
Guo
John
J
Luk
Marcel Wing Leung
MW
Koo
D019342:Acetic Acid; D000818:Animals; D000897:Anti-Ulcer Agents / Q000032:analysis / Q000627:therapeutic use*; D002455:Cell Division / Q000187:drug effects; D028041:Centella / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects; D008297:Male; D018919:Neovascularization, Physiologic; D009195:Peroxidase / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D014315:Triterpenes / Q000032:analysis / Q000627:therapeutic use*
D000897:Anti-Ulcer Agents; D010936:Plant Extracts; D014315:Triterpenes; D016222:Fibroblast Growth Factor 2; D009195:Peroxidase; C004446:asiaticoside; D019342:Acetic Acid