Antigenotoxic effect of grape seed procyanidin extract in Fao cells submitted to oxidative stress.
The protective effects of grape seed procyanidin extract on the repair of H(2)O(2)-induced DNA lesions were tested using Fao cells. Cells were exposed to 600 microM H(2)O(2) for 3 or 21 h. A procyanidin extract from grape seed (PE) was incubated or preincubated (1 h) during the exposure to H(2)O(2). The ability of procyanidins to protect against the genotoxicity of H(2)O(2) was compared with those of the monomeric flavanols (+)-catechin and (-)-epicatechin and the flavonol quercetin. After treatment, DNA damage was monitored using alkaline single-cell gel electrophoresis (the comet assay) (Aherne, S. A.; O'Brien, N. M. Nutr. Cancer 1999, 34, 160-166). At the end of the experiment, PE significantly decreased the damage caused by H(2)O(2). The results also showed that quercetin was the most effective of the flavonoids tested, which is consistent with its powerful antioxidant character. The results indicate that procyanidins are more effective than the corresponding individual monomers, catechin and epicatechin, at preventing DNA lesions in hepatocytes and that this protection is higher after preincubation than after co-incubation.
Departament de Bioquímica i Biotecnología, Unitat d'Enologia del Centre de Referència en Tecnologia dels Aliments de la Generalitat de Catalunya, Universitat Rovira i Virgili, Imperial Tàrraco 1, 43005 Tarragona, Spain.
Niurka
N
Llópiz
Francesc
F
Puiggròs
Ela
E
Céspedes
Lluís
L
Arola
Anna
A
Ardévol
Cinta
C
Bladé
M Josepa
MJ
Salvadó
D016587:Antimutagenic Agents / Q000494:pharmacology*; D044946:Biflavonoids; D002392:Catechin / Q000494:pharmacology*; D002460:Cell Line; D004249:DNA Damage / Q000187:drug effects; D022781:Hepatocytes / Q000737:chemistry / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D018384:Oxidative Stress; D010936:Plant Extracts / Q000494:pharmacology; D044945:Proanthocyanidins; D011794:Quercetin / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry*; D027843:Vitis / Q000737:chemistry*
D016587:Antimutagenic Agents; D044946:Biflavonoids; D010936:Plant Extracts; D044945:Proanthocyanidins; C017674:procyanidin; D002392:Catechin; D011794:Quercetin; D006861:Hydrogen Peroxide