[Cloning and structural analysis of 5'-flanking sequence of human hepatic stimulator substance (hHSS) gene].
Human hepatic stimulator substance (hHSS) is a newly identified growth-promoting factor in liver. HSS is capable of stimulating the hepatic regeneration in hepaectomized rats and promoting the growth of hepatic tumor cells. To understand and elucidate the regulation of hHSS on hepatocyte growth at genetic level, the 4,890 bp of 5'-flanking region of hHSS gene have been isolated and sequenced. On basis of comparison of the 5'-flanking sequences with GenBank, hHSS gene was localized on human chromosome 16. Transcriptional start site was identified by reverse transcriptase-polymerase chain reaction (RT-PCR) and 5' rapid amplification of cDNA end (5'RACE). The initiation nucleotide was defined as 'C', which located at 254 bp upstream of the reported sequence of exon I.
Department of Cell Biology, Capital University of Medical Sciences, Beijing 100054, China.
Xin-Nan
XN
Wang
Zhi-Gang
ZG
Song
Yuan
Y
Wu
Li
L
Chen
Yan-Yan
YY
Zhao
Wei
W
An
D024506:5' Flanking Region; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics*; D014158:Transcription, Genetic
D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C073456:hepatic stimulator substance