The latency-related gene encoded by bovine herpesvirus 1 can suppress caspase 3 and caspase 9 cleavage during productive infection.
When the bovine herpesvirus 1 (BHV-1) latency-related (LR) gene is inserted into the latency-associated transcript (LAT) locus of a herpes simplex virus type 1 (HSV-1) LAT deletion mutant, high levels of spontaneous reactivation from latency and enhanced pathogenesis occur. The LR gene, but not LAT, inhibits caspase 3 cleavage during productive infection. Plasmids containing LAT or the LR gene inhibit caspase 3 activation in transiently transfected cells, suggesting productive infection blocks certain antiapoptotic properties of LAT. These studies demonstrate a correlation between the enhanced pathogenic potential of CJLAT and the LR gene inhibiting caspase 3 cleavage during productive infection.
Henderson
Gail
G
Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, Lincoln, Nebraska 68583-0905, USA.
Perng
Guey-Chuen
GC
Nesburn
Anthony B
AB
Wechsler
Steven L
SL
Jones
Clinton
C
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D015153:Blotting, Western; D053148:Caspase 3; D053453:Caspase 9; D020169:Caspases / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D015967:Gene Expression Regulation, Viral; D007242:Herpesvirus 1, Bovine / Q000472:pathogenicity*; D009474:Neurons / Q000821:virology; D012322:RNA Precursors / Q000235:genetics*; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics*; D017735:Virus Latency / Q000235:genetics*
D012322:RNA Precursors; D014764:Viral Proteins; C401822:latency-related protein, Bovine herpesvirus 1; D053148:Caspase 3; D053453:Caspase 9; D020169:Caspases