The metNPQ operon of Bacillus subtilis encodes an ABC permease transporting methionine sulfoxide, D- and L-methionine.
The Bacillus subtilis yusCBA operon, which encodes an ABC-type transporter, contains an S-box motif in its promoter region. We showed that the expression of these genes is repressed via the S-box system when methionine is available. The YusCB proteins are involved in the transport of both d- and l-methionine but also methionine sulfoxide. A yusCB mutant is unable to grow in the presence of 5 microM l-methionine or 100 microM methionine sulfoxide, while it grows similarly to the wild type with 100 microM l-methionine and 1 mM methionine sulfoxide. Other uptake systems are therefore present for these two compounds. In contrast, the Yus ABC transporter corresponds to the sole d-methionine uptake system. We propose to rename yusC, yusB and yusA as metN, metP and metQ, respectively.
Hullo
Marie-Françoise
MF
Unité de Génétique des Génomes Bactériens, Institut Pasteur, URA CNRS 2171, 28, rue du Docteur Roux, 75724 Paris Cedex 15, France.
Auger
Sandrine
S
Dassa
Elie
E
Danchin
Antoine
A
Martin-Verstraete
Isabelle
I
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism; D026905:Amino Acid Transport Systems / Q000235:genetics / Q000378:metabolism; D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism; D008715:Methionine / Q000031:analogs & derivatives* / Q000378:metabolism*; D009876:Operon / Q000235:genetics
D018528:ATP-Binding Cassette Transporters; D026905:Amino Acid Transport Systems; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C466430:MetQ protein, E coli; D008715:Methionine; D000251:Adenosine Triphosphatases; C466428:MetN protein, E coli; C013111:methionine sulfoxide