Immunohistochemical staining of endomorphin 1 and 2 in the immune cells of the spleen.
Endomorphin 1 (EM-1) and EM-2 have been widely reported in the cells of the central nervous system (CNS) but limited research has been done regarding their distribution in the peripheral system. The occurrence of EM-1 and -2 in the spleen as measured by RIA and their ability to mediate immune function imply a role for EMs in this area. The current study examines the localization of EM-1 and -2 in the immune cells of the spleen of male and female rats via an immunohistochemical procedure. In both genders, EM-1 and -2 immunoreactive staining was predominantly present in macrophages and B cells with minimal EM immunoreactive staining in T cells. This is the first evidence of a differential distribution of EM-1 and -2 in cells of the immune system.
Laboratories for Integrative Neuroscience and Endocrinology, Dorothy Hodgkin Building, Whitson Street, Bristol BS1 3NY, UK. j.v.seale@bristol.ac.uk
J V
JV
Seale
D S
DS
Jessop
M S
MS
Harbuz
D000818:Animals; D005260:Female; D007150:Immunohistochemistry; D008264:Macrophages / Q000737:chemistry; D008297:Male; D009842:Oligopeptides / Q000032:analysis*; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000737:chemistry* / Q000166:cytology
D009842:Oligopeptides; C105293:endomorphin 1; C105294:endomorphin 2