Localization of the Na+-D-glucose cotransporter SGLT1 in the blood-brain barrier.
Immunoreactivity of the Na+-D-glucose cotransporter SGLT1 was demonstrated in intracerebral capillaries of rat and pig. Immunostaining suggested that SGLT1 is located in the luminal membrane of the endothelial cells and in intracellular vesicles. Using in situ hybridization, SGLT1 mRNA was not detectable in intracerebral capillaries of non-treated or sham-operated Wistar rats. However, 1 day after a transient occlusion of the right middle cerebral artery, SGLT1 mRNA was detected in capillaries of both brain hemispheres. Expression of SGLT1 was also demonstrated in primary cultures of capillary endothelial cells from pig using polymerase chain reaction after reverse transcription and western blotting. The data suggest that SGLT1 participates in transport of D-glucose across the blood-brain barrier and is upregulated after brain ischemia and reperfusion.
Institute of Anatomy and Cell Biology, University Würzburg, Koellikerstrasse 6, 97070 Würzburg, Germany.
Katrin
K
Elfeber
Alwin
A
Köhler
Michael
M
Lutzenburg
Christina
C
Osswald
Hans-Joachim
HJ
Galla
Otto W
OW
Witte
Hermann
H
Koepsell
D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D051269:Sodium-Glucose Transporter 1; D013552:Swine; D015854:Up-Regulation
D008562:Membrane Glycoproteins; D009004:Monosaccharide Transport Proteins; C495676:Slc5a1 protein, rat; D051269:Sodium-Glucose Transporter 1; D005947:Glucose