Purification and characterization of a new peptide antibiotic produced by a thermotolerant Bacillus licheniformis strain.
A Bacillus licheniformis strain, 189, isolated from a hot spring environment in the Azores, Portugal, strongly inhibited growth of Gram-positive bacteria. It produced a peptide antibiotic at 50 degrees C. The antibiotic was purified and biochemically characterized. It was highly resistant to several proteolytic enzymes. Additionally, it retained its antimicrobial activity after incubation at pH values between 3.5 and 8; it was thermostable, retaining about 85% and 20% of its activity after 6 h at 50 degrees C and 100 degrees C, respectively. Its molecular mass determined by mass spectrometry was 3249.7 Da.
Mendo
Sónia
S
Departamento de Biologia, Centro de Biologia Celular, Universidade de Aveiro, 3810-193 Aveiro, Portugal. smendo@bio.ua.pt
Faustino
Nuno André
NA
Sarmento
Ana Cristina
AC
Amado
Francisco
F
Moir
Arthur J G
AJ
D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001394:Azores; D001407:Bacillus / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry; D010455:Peptides
D000900:Anti-Bacterial Agents; D010455:Peptides; D010450:Endopeptidases