Genome resource for the Indonesian coelacanth, Latimeria menadoensis.
We have generated a BAC library from the Indonesian coelacanth, Latimeria menadoensis. This library was generated using genomic DNA of nuclei isolated from heart tissue, and has an average insert size of 171 kb. There are a total of 288 384-well microtiter dishes in the library (110,592 clones) and its genomic representation is estimated to encompass > or = 7X coverage based on the amount of DNA presumably cloned in the library as well as via hybridization with probes to a small set of single copy genes. This genomic resource has been made available to the public and should prove useful to the scientific community for many applications, including comparative genomics, molecular evolution and conservation genetics.
Danke
Joshua
J
Benaroya Research Institute at Virginia Mason, Seattle, Washington 98101, USA.
Miyake
Tsutomu
T
Powers
Thomas
T
Schein
Jacqueline
J
Shin
Heesun
H
Bosdet
Ian
I
Erdmann
Mark
M
Caldwell
Roy
R
Amemiya
Chris T
CT
D000818:Animals; D022202:Chromosomes, Artificial, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005399:Fishes / Q000235:genetics*; D015698:Genomic Library