Neutrophil chemotaxis and receptor expression in clinical septic shock.
OBJECTIVE
To examine the hypothesis that neutrophil chemotaxis to interleukin-8 (IL-8) is reduced in septic shock. Surface expression of neutrophil CXC chemokine receptors and the adhesion molecule CD11b were also examined and associations between disease severity, gas exchange and receptor expression were studied.


METHODS
Prospective cohort clinical study. SETTING. Intensive care unit in a tertiary referral teaching hospital.


METHODS
Patients with septic shock ( n=15) and healthy controls ( n=8) were studied.


RESULTS
Daily (for 5 consecutive days) flow cytometric measurements of chemokine and beta integrin surface expression. "In vitro" neutrophil chemotaxis to IL-8 was also compared between patients with sepsis and healthy controls. CXCR2 expression significantly fell, CD11b expression increased and CXCR1 expression was unchanged throughout the study in the septic group compared with healthy controls. CD11b positively correlated with increasing APACHE II scores ( p<0.0001) and worsening PaO(2)/FIO(2) ratios ( p<0.0001). CXCR2 expression negatively correlated with both APACHE II scores ( p=0.016) and PaO(2)/FIO(2) ratios ( p=0.01). There was no correlation between CXCR1 expression and either APACHE II score or PaO(2)/FIO(2) ratios. Chemotaxis to IL-8 was reduced in patients with sepsis compared with healthy volunteers.


CONCLUSIONS
Surface expression of the chemokine receptor CXCR2 and the beta-integrin CD11b, but not CXCR1, were reduced on neutrophils isolated from patients with septic shock compared with healthy controls. Chemotaxis to IL-8 was also reduced in neutrophils from septic patients compared with healthy controls. The changes in receptor expression correlated with measures of disease severity.
University Departments of Reproductive and Surgical Sciences, University of Newcastle upon Tyne, Framlington Place, NE1 7RU, Newcastle upon Tyne, UK.
Ahmad D
AD
Chishti
Brian K
BK
Shenton
John A
JA
Kirby
Simon V
SV
Baudouin
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D039481:CD11b Antigen / Q000097:blood; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D015331:Cohort Studies; D005260:Female; D005434:Flow Cytometry; D006784:Hospitals, Teaching; D006801:Humans; D007362:Intensive Care Units; D016209:Interleukin-8 / Q000097:blood / Q000494:pharmacology; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism* / Q000473:pathology; D011379:Prognosis; D011446:Prospective Studies; D019707:Receptors, Chemokine / Q000032:analysis*; D012720:Severity of Illness Index; D012772:Shock, Septic / Q000378:metabolism / Q000401:mortality / Q000503:physiopathology*; D015996:Survival Rate
D039481:CD11b Antigen; D016209:Interleukin-8; D019707:Receptors, Chemokine