Trimethyltin-induced differential expression of PAR subtypes in reactive astrocytes of the rat hippocampus.
Thrombin, its main inhibitor (protease nexin-1) and its related receptors (protease-activated receptors, PAR-1,-2, -3, -4) were studied in rat hippocampus following administration of trimethyltin (TMT), a neurotoxin inducing neuronal degeneration and reactive gliosis. Reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry revealed that while expression of prothrombin and protease nexin-1 did not change significantly in TMT-treated hippocampi, PARs (in particular PAR-1 and to a lesser extent PAR-2 and PAR-3) were upregulated in reactive astrocytes, suggesting their involvement in neurodegeneration and in the consequent response of the nervous tissue.
Department of Cardiovascular Sciences, University "La Sapienza", Via A. Borelli 50, Rome 00161, Italy. elena.pompili@uniroma1.it
Elena
E
Pompili
Stefania Lucia
SL
Nori
Maria Concetta
MC
Geloso
Enrico
E
Guadagni
Valentina
V
Corvino
Fabrizio
F
Michetti
Lorenzo
L
Fumagalli
D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects; D007150:Immunohistochemistry / Q000379:methods; D009498:Neurotoxins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D044462:Receptors, Proteinase-Activated / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014298:Trimethyltin Compounds / Q000494:pharmacology*; D015854:Up-Regulation
D009498:Neurotoxins; D012333:RNA, Messenger; D044462:Receptors, Proteinase-Activated; D014298:Trimethyltin Compounds; C046488:trimethyltin