Extracellular levels of amino acids and choline in human high grade gliomas: an intraoperative microdialysis study.
The concentrations of endogenous amino acids and choline in the extracellular fluid of human cerebral gliomas have been measured, for the first time, by in vivo microdialysis. Glioblastoma growth was associated with increased concentrations of choline, GABA, isoleucine, leucine, lysine, phenylalanine, taurine, tyrosine, and valine. There was no difference between grade III and grade IV tumors in the concentrations of phenylalanine, isoleucine, tyrosine, valine, and lysine, whereas the concentrations of choline, aspartate, taurine, GABA, leucine, and glutamate were significantly different in the two tumor-grade subgroups. In contrast to the other compounds, the concentration of glutamate was decreased in glioma. The parenchyma adjacent to the tumor showed significant changes only in the extracellular concentration of glutamate, isoleucine, and valine. The concentrations of choline and the amino acids, glutamate, leucine, taurine, and tyrosine showed significant positive correlations with the degree of cell proliferation. Epilepsy, which is relatively common in subjects with gliomas, was shown to be a significant confounding variable when the extracellular concentrations of aspartate, glutamate and GABA were considered.
Dipartimento di Farmacologia Preclinica e Clinica M. Aiazzi Mancini, Università di Firenze, Viale G. Pieraccini 6, 50139 Firenze, Italy.
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Bianchi
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De Micheli
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Bricolo
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Ballini
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Fattori
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Venturi
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Pedata
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KF
Tipton
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Della Corte
D000328:Adult; D000368:Aged; D000596:Amino Acids / Q000378:metabolism*; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D002455:Cell Division; D002794:Choline / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D005910:Glioma / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D006801:Humans; D007432:Intraoperative Period; D008297:Male; D017551:Microdialysis; D008875:Middle Aged
D000596:Amino Acids; D002794:Choline