Liquid chromatographic determination of 4,4'-dinitrocarbanilide, the active component of the infertility agent nicarbazin, in chicken, duck, goose, and snake eggs.
4,4'-Dinitrocarbanilide (DNC) was extracted from chicken, duck, goose, and snake eggs and isolated by reversed-phase liquid chromatography. DNC was detected by ultraviolet absorbance at 347 nm and quantitated by comparison with a calibration standard. Recoveries of DNC from fortified control chicken, duck, goose, and snake egg samples were determined for DNC levels of 0.16, 10, and 16 microg/g. The mean recoveries from chicken, duck, goose, and snake eggs were 92 +/- 4, 88 +/- 9, 87 +/- 7, and 95 +/- 6%, respectively. The method limits of detection for DNC in chicken, duck, goose, and snake eggs ranged from 0.015 to 0.035 microg/g. The reported method is much simpler than and equally efficient as previous methods developed for the determination of DNC residues in egg contents.
US Department of Agriculture, Animal and Plant Health Inspection Service, National Wildlife Research Center, 4101 LaPorte Ave, Ft Collins, Denver, CO 80521, USA. thomas.m.primus@aphis.usda.gov
Thomas M
TM
Primus
Dennis J
DJ
Kohler
Margaret A
MA
Goodall
Christi
C
Yoder
Thomas
T
Mathies
Lowell
L
Miller
John J
JJ
Johnston
Kurt
K
Vercauteren
D000818:Animals; D002138:Calibration; D002224:Carbanilides / Q000032:analysis*; D002645:Chickens; D004372:Ducks; D004531:Eggs / Q000032:analysis*; D005299:Fertility Agents / Q000032:analysis*; D005777:Geese; D007202:Indicators and Reagents; D009528:Nicarbazin / Q000032:analysis*; D012015:Reference Standards; D012911:Snakes; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
D002224:Carbanilides; D005299:Fertility Agents; D007202:Indicators and Reagents; D012996:Solutions; D009528:Nicarbazin; C039493:4,4'-dinitrocarbanilide