Acute and topic anti-edematogenic fractions isolated from the seeds of Pterodon pubescens.
We previously demonstrated that alcoholic extracts from Pterodon pubescens Benth. (Sucupira branca, Leguminosae) seeds exhibit anti-arthritic activity. In the present work we show that the oleaginous extract obtained from P. pubescens seeds (OEP) exhibits acute or topic anti-edematogenic activity when tested in carrageenan-induced paw edema or in croton oil-induced ear edema assays, respectively. Four fractions were obtained from OEP by sequential liquid-liquid extraction. The anti-edematogenic properties were predominant in the hexanic fraction, which was further fractionated by HPLC, yielding three sub-fractions (PF1.1, PF1.2 and PF1.3). PF1.1 and PF1.3 showed potent acute and topic anti-edematogenic activity. The PF1.2 sub-fraction, although not active in the carrageenan assay, exhibited a potent anti-edematogenic activity in the croton oil-induced ear edema. This sub-fraction shows a maximum efficacy similar to indometacin in a lower dose. The PF1.1 sub-fraction presented a complex mixture containing furane diterpene derivatives of vouacapan. PF1.2 consists of a single substance, geranylgeraniol, as determined by GC/MS and NMR, while PF1.3 contains farnesol.
Departamento de Bioqímica, Instituto de Biologia, Universidade do Estado do Rio de Janeiro, Av Professor Manoel de Abreu, 44, PAPC, 4o andar, CEP 20550-170, Rio de Janeiro, RJ, Brazil.
M C C
MC
Silva
C R M
CR
Gayer
C S
CS
Lopes
N O
NO
Calixto
P A
PA
Reis
C P B
CP
Passaes
M C
MC
Paes
S R
SR
Dalmau
K C C
KC
Sabino
A R
AR
Todeschini
M G P
MG
Coelho
D000287:Administration, Topical; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004487:Edema / Q000188:drug therapy*; D007887:Fabaceae; D008401:Gas Chromatography-Mass Spectrometry; D007274:Injections, Intraperitoneal; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000627:therapeutic use; D012639:Seeds
D010936:Plant Extracts