[Application of collagen-binding assay for von Willebrand disease].
OBJECTIVE
To explore the application of collagen-binding assay for von Willebrand disease (vWD).


METHODS
We investigated the sensitivity and reproducibility of collagen-binding assays (CBA) of vWF using the enzyme-linked immunosorbent assay (ELISA) technique and compared CBA with 3 other assays for their ability to detect vWD.


RESULTS
It was found that the vWF: CBA level of plasma from patients with different types of vWD is lower than that from healthy donors, hemophilia and other bleeding disorders (P < 0.001). In general, the four assays have the capability to identify vWD from normal and other bleeding disorders, and can be used for diagnosing vWD. The concordance rates of the four assays on vWD are vWF: Ag 85.7%, vWF: Rcof 76.2%, RIPA 80.9%, vWF: CBA 95.2% respectively.


CONCLUSIONS
vWF: CBA measured by ELISA is highly sensitive, specific and reproducible, and easy to operate. The measurement of the functional activity of vWF by vWF: Rcof or RIPA can be replaced by the more reliable vWF: CBA.
Zhou
Jing
J
Department of Clinical Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Jia
Yong-qian
YQ
Jiang
Hong
H
Yang
Yi-ming
YM
Deng
Cheng-qi
CQ
D001681:Biological Assay; D003094:Collagen / Q000032:analysis* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006467:Hemophilia A / Q000097:blood; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014842:von Willebrand Diseases / Q000097:blood*; D014841:von Willebrand Factor / Q000032:analysis* / Q000378:metabolism
D014841:von Willebrand Factor; D003094:Collagen