Identification of neuropeptides from the sinus gland of the crayfish Orconectes limosus using nanoscale on-line liquid chromatography tandem mass spectrometry.
In this article, a novel and sensitive analytical strategy for direct characterization of neuropeptides from the X-Organ-sinus gland neurosecretory system of the crayfish Orconectes limosus is presented. A desalted extract corresponding to 0.5 sinus gland equivalents was analyzed in a nanoflow liquid chromatography system coupled to quadrupole time-of-flight tandem mass spectrometry (nanoLC-QTOF MS/MS). The existence and structural identity of four crustacean hyperglycemic hormone precursor-related peptide variants and two new genetic variants of the pigment-dispersing hormone, not detected by conventional chromatographic systems, molecular cloning, or immunochemical methods before, was revealed. The here-presented approach of the combined LC-QTOF MS/MS technique is a powerful tool to discover new peptide hormones in biological systems, due to its sensitivity, accuracy, and speed.
Institute for Zoophysiology, University of Bonn, Endenicher Allee 11-13, D-53115 Bonn, Germany.
Patrick
P
Bulau
Iris
I
Meisen
Tina
T
Schmitz
Rainer
R
Keller
Jasna
J
Peter-Katalinić
D000818:Animals; D003400:Astacoidea / Q000737:chemistry*; D002853:Chromatography, Liquid; D013058:Mass Spectrometry; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000378:metabolism; D009479:Neuropeptides / Q000737:chemistry* / Q000378:metabolism; D009490:Neurosecretory Systems / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry*; D011498:Protein Precursors / Q000737:chemistry*
D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D010446:Peptide Fragments; D011498:Protein Precursors; C061766:hyperglycemic hormone precursor, crustacean