Capillary zone electrophoresis investigation of the interaction between heparin and granulocyte-colony stimulating factor.
The interaction between standard heparin, low-molecular-weight heparin (LMWH), and granulocyte-colony stimulating factor (G-CSF) was studied by capillary zone electrophoresis. Both qualitative and quantitative characterizations of the heparin-protein binding were determined. The binding constants of the two different groups of heparins with G-CSF, calculated from the Scatchard plot by regression, were 4.805 x 10(5) M(-1) and 4.579 x 10(5) M(-1), respectively. The two binding constants measured are of the same order of magnitude at 10(5) M(-1), indicating that LMWH contains most of the functional groups bound to G-CSF by standard heparin.
Liang
Aiye
A
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, P. R. China.
He
Xinya
X
Du
Yuguang
Y
Wang
Keyi
K
Fung
Yingsing
Y
Lin
Bingcheng
B
D019075:Electrophoresis, Capillary / Q000379:methods*; D016179:Granulocyte Colony-Stimulating Factor / Q000737:chemistry* / Q000378:metabolism*; D006493:Heparin / Q000737:chemistry* / Q000378:metabolism*; D006495:Heparin, Low-Molecular-Weight / Q000737:chemistry / Q000378:metabolism; D009994:Osmolar Concentration; D011485:Protein Binding
D006495:Heparin, Low-Molecular-Weight; D016179:Granulocyte Colony-Stimulating Factor; D006493:Heparin