Activity of immobilized papain dehydrated by n-propanol in low-water media.
A propanol-rinsed enzyme preparations (PREP) of papain showed an activity of 59 nmol min(-1) (mg powder)(-1) in tert-butanol at the optimal water activity of 0.2. The immobilized papain was stable in aqueous media for 3 d at 4 degrees C. Solid-state buffers (bases and their HCl salts) suspended in the organic medium decreased the initial rate in all cases tested. The operational stability during Z-Gly-Phe-NH2 synthesis was improved when solid cysteine was added, doubling the yield after 24 h.
Theppakorn
Theerapong
T
Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Kanasawud
Pawinee
P
Halling
Peter J
PJ
D000433:1-Propanol / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry*; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry*; D010206:Papain / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*
D004800:Enzymes, Immobilized; D012997:Solvents; D000433:1-Propanol; D010206:Papain; D003545:Cysteine