Solid-state NMR spectroscopy of aligned lipid bilayers at low temperatures.
This study, for the first time, demonstrates that it is possible to mechanically align lipid bilayers at a very low temperature (as low as the gel-to-liquid crystalline phase transition temperature). Performing NMR experiments on mechanically aligned lipid bilayers at a low temperature increases the signal-to-noise ratio, the resolution, and the span of NMR parameters. The increased lifetime of the alignment and the nature of the bilayer sample would enhance the application of solid-state NMR techniques to study membrane proteins.
Lee
Dong-Kuk
DK
Department of Chemistry, Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109-1055, USA.
Wildman
Katherine Henzler
KH
Ramamoorthy
Ayyalusamy
A
D003080:Cold Temperature; D003903:Deuterium; D008051:Lipid Bilayers / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010713:Phosphatidylcholines / Q000737:chemistry; D014867:Water / Q000737:chemistry
D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D014867:Water; D003903:Deuterium; C028694:1-palmitoyl-2-oleoylphosphatidylcholine