RpS3, a DNA repair endonuclease and ribosomal protein, is involved in apoptosis.
It is known that mammalian rpS3 functions as a DNA repair endonuclease and ribosomal protein S3. It was also observed that several ribosomal proteins or DNA repair enzymes are related to apoptosis. We report here a third function of rpS3, induction of apoptosis. The localization of green fluorescent protein (GFP)-rpS3 is changed to the nuclear membrane when lymphocytic cells undergo rpS3-induced apoptosis. Transient expression of GFP-rpS3 activates caspase-8/caspase-3 and sensitizes cytokine-induced apoptosis. Deletion analysis reveals that the two functions of rpS3, DNA repair and apoptosis, use independent functional domains.
Jang
Chang-Young
CY
Laboratory of Biochemistry, School of Life Sciences & Biotechnology, and BioInstitute, Korea University, Seoul 136-701, South Korea.
Lee
Jae Yung
JY
Kim
Joon
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D017209:Apoptosis; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D004260:DNA Repair; D003850:Deoxyribonuclease I / Q000378:metabolism*; D004789:Enzyme Activation; D000939:Epitopes; D017353:Gene Deletion; D049452:Green Fluorescent Proteins; D006801:Humans; D019169:Jurkat Cells; D008164:Luminescent Proteins; D051379:Mice; D010954:Plasmacytoma; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000378:metabolism; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D008164:Luminescent Proteins; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C044152:ribosomal protein S3; D049452:Green Fluorescent Proteins; D003850:Deoxyribonuclease I; D020169:Caspases