Evaluation of penicillin binding protein 2a latex agglutination assay for identification of methicillin-resistant Staphylococcus aureus directly from blood cultures.
The penicillin binding protein 2a (PBP2a) latex agglutination test using a blood culture pellet was compared to the oxacillin screen agar method using isolated colonies. For blood cultures positive for Staphylococcus aureus (n = 70), the direct PBP2a test was 18% sensitive and 100% specific. The PBP2a test shows poor sensitivity when used directly with positive blood cultures.
Department of Pathology, Lifespan Academic Medical Centers, Rhode Island, USA. kchapin@lifespan.org
Kimberle C
KC
Chapin
Michael C
MC
Musgnug
D001426:Bacterial Proteins / Q000032:analysis*; D002352:Carrier Proteins / Q000032:analysis*; D006602:Hexosyltransferases / Q000032:analysis*; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods*; D016106:Methicillin Resistance; D002267:Muramoylpentapeptide Carboxypeptidase / Q000032:analysis*; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases / Q000032:analysis*; D016133:Polymerase Chain Reaction; D013290:Streptococcal Infections / Q000175:diagnosis / Q000382:microbiology
D001426:Bacterial Proteins; D002352:Carrier Proteins; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase