Effect of dietary enrichment with n-3 polyunsaturated fatty acids (PUFA) or n-9 PUFA on arachidonate metabolism in vivo and experimentally induced inflammation in mice.
Mice were fed a diet supplemented with palm oil (control diet), n-3 polyunsaturated fatty acids (PUFA)-, or n-9 PUFA-rich oil for 3 weeks. The n-3 PUFA-rich diet suppressed the generation of both leukotrienes (LT) and prostaglandins (PG), but the n-9 PUFA-rich diet did LT but not PG generation during acute inflammation. Leukocyte accumulation during acute inflammation was not different in the n-3 or n-9 PUFA-rich diet group as compared with the control group. The n-3 PUFA-rich diet but not the n-9 PUFA-rich diet suppressed Freund's adjuvant-induced granuloma formation. The n-9 PUFA-rich diet significantly attenuated galactosamine/lipopolysaccharide-induced liver injury more effectively than the n-3 PUFA-rich diet as compared with the control diet. The present study revealed the differential modification of experimentally induced inflammation in mice by dietary n-3 PUFA and n-9 PUFA, which may be due to their different effects on 5-lipoxygenease and cyclooxygenase metabolism of arachidonic acid during inflammatory processes.
Doshi
Masaru
M
Division of Clinical Application, Department of Clinical Sciences, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Sugitani, Toyama, Japan.
Watanabe
Shiro
S
Niimoto
Tsuyoshi
T
Kawashima
Hiroshi
H
Ishikura
Yoshiyuki
Y
Kiso
Yoshinobu
Y
Hamazaki
Tomohito
T
D000208:Acute Disease; D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism; D016718:Arachidonic Acid / Q000378:metabolism*; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D004032:Diet; D015525:Fatty Acids, Omega-3; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005260:Female; D005620:Freund's Adjuvant; D005688:Galactosamine; D006099:Granuloma / Q000209:etiology / Q000517:prevention & control; D007249:Inflammation / Q000188:drug therapy* / Q000378:metabolism; D007958:Leukocyte Count; D008055:Lipids / Q000737:chemistry; D008070:Lipopolysaccharides; D016859:Lipoxygenase Inhibitors; D008107:Liver Diseases / Q000201:enzymology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010529:Peritoneal Cavity / Q000166:cytology; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D014280:Triglycerides / Q000494:pharmacology*
D016861:Cyclooxygenase Inhibitors; D015525:Fatty Acids, Omega-3; D005231:Fatty Acids, Unsaturated; D008055:Lipids; D008070:Lipopolysaccharides; D016859:Lipoxygenase Inhibitors; D014280:Triglycerides; C077446:Pikasol; D016718:Arachidonic Acid; D005688:Galactosamine; D005620:Freund's Adjuvant; D001094:Arachidonate 5-Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases