Successful extraction of human genomic DNA from serum and its application to forensic identification.
We report here on the successful extraction of human genomic DNA from a serum sample in a forensic case. The extracted DNA was successfully used for the identification of remains presumably immersed for more than three weeks for which the only comparison sample was a 250-microL serum aliquot kept frozen in a laboratory. The analysis made it possible to identify a second victim as the daughter of the first.
Ravard-Goulvestre
Claire
C
Laboratoire de Biochimie et Biologie Moléculaire, Hôpital Raymond Poincaré, 92380 Garches, France.
Crainic
Karine
K
Guillon
François
F
Paraire
François
F
Durigon
Michel
M
de Mazancourt
Philippe
P
D000328:Adult; D000483:Alleles; D002648:Child; D004247:DNA / Q000097:blood* / Q000302:isolation & purification*; D017931:DNA Primers; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D015894:Genome, Human; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D011180:Postmortem Changes; D020080:Tandem Repeat Sequences
D017931:DNA Primers; D004247:DNA