Coils and tubes releasing heparin. Studies on a new vascular graft prototype.
Coiled metallic guidewires find widespread use, for instance in interventional cardiology. It is known that release of heparin from the surface of guidewires is advantageous to prevent formation of thrombotic emboli. New coiled tubular structures, having larger inner and outer diameter as compared to guidewires, are presented. In theory these tubes can be used as interposition vascular grafts. Ten coiled tubes with an internal diameter of 690 microm were made. Five different adherent polymeric coatings with increasing hydrophilicity were used. Five tubes contained heparin in the coating and the other five were unheparinised controls. The five tubes containing heparin were studied with respect to heparin release in vitro (amount released, kinetics), and immobilised heparin that is exposed at the surface. All tubes were studied with a direct cell contact assay using 3T3 mouse fibroblast cells, a dynamic thrombin generation test, and endothelial cell growth onto the coils. It was found that the heparinised tubes lead to very little thrombin formation. It is argued that this is due to heparin that is immobilised and exposed at the inner surface of such tubes. Furthermore the coils showed to be cytocompatible and endothelial cells adhere and proliferate well onto the coils. This concept is believed to hold promise for further development of small vascular grafts.
Center for Biomaterials Reasearch, University of Maastricht, P.O. Box 616, 6200 MD Maastricht, The Netherlands. y.aldenhoff@bioch.unimaas.nl
Yvette B J
YB
Aldenhoff
Menno L W
ML
Knetsch
Johannes H L
JH
Hanssen
Theo
T
Lindhout
Simone J H
SJ
Wielders
L H Leo H
LH
Koole
D016475:3T3 Cells; D000818:Animals; D001807:Blood Vessel Prosthesis; D002404:Catheterization / Q000295:instrumentation* / Q000379:methods; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000295:instrumentation* / Q000379:methods; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D019544:Equipment Failure Analysis; D006493:Heparin / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D051379:Mice; D010865:Pilot Projects; D011474:Prosthesis Design; D013927:Thrombosis / Q000517:prevention & control; D019737:Transplants
D020099:Coated Materials, Biocompatible; D006493:Heparin