Assessment of methods of destruction of Syphacia muris eggs.
An investigation was performed to identify methods suitable for decontamination of equipment being transferred from a facility contaminated with Syphacia muris to a new facility. Perianal samples were taken on clear sticky tape from rats known to be infected with S. muris. Tapes and attached worm eggs were treated with ethylene oxide, formaldehyde fumigation, potassium peroxysulphate, chlorine dioxide and didecyl di-methyl ammonium chloride, chlorine dioxide, alcohol/chlorhexidine, 100 degrees C dry heat for 30 min, ultraviolet light (UV), or left for 4 weeks at room temperature. The subsequent viability of the eggs was compared to untreated control samples. Heat and ethylene oxide produced a 100% kill rate of S. muris eggs. Formaldehyde gas and chlorine dioxide treatments showed marked effectiveness, with the number of eggs killed at 94% and 96%, respectively, while the other agents showed less efficacy (36-78% eggs killed). The percentage of eggs not hatching in the controls was 21%. Since so few agents were effective against S. muris eggs, care should be taken when choosing a method for decontaminating a facility.
Department of Laboratory Animal Science, GlaxoSmithKline Pharmaceuticals, The Frythe, Welwyn, Hertfordshire AL6 9AR, UK. jacqueline.dix@gsk.com
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Dix
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Astill
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Whelan
D001003:Anal Canal / Q000469:parasitology; D000818:Animals; D003666:Decontamination / Q000379:methods*; D004202:Disinfectants / Q000494:pharmacology; D004864:Equipment and Supplies / Q000469:parasitology*; D005027:Ethylene Oxide / Q000494:pharmacology; D006358:Hot Temperature; D010063:Ovum / Q000187:drug effects*; D010123:Oxyuriasis / Q000517:prevention & control / Q000635:transmission / Q000662:veterinary*; D010124:Oxyuroidea / Q000196:embryology / Q000302:isolation & purification; D051381:Rats; D012376:Rodent Diseases / Q000469:parasitology* / Q000517:prevention & control / Q000635:transmission
D004202:Disinfectants; D005027:Ethylene Oxide