Identification and quantitation by high-performance liquid chromatography of mancozeb following derivatization by 1,2-benzenedithiol.
Ethylenebisdithiocarbamate (EBDC) fungicides are the most important class of organic fungicides and exhibit a high degree of carcinogenicity, mutagenicity, and neurotoxicity. For that reason, the safe application of these fungicides in practice requires a convenient method for their determination, applicable to biological fluids. We describe a high-performance liquid chromatography (HPLC) assay. After elimination of the metal which defines the product (maneb, mancozeb, zineb.) with EDTA, the resulting EBDC is derivatized with 1,2-benzenedithiol to yield a cyclocondensation product, 1,3-benzodithiole-2-thione, which can then be quantitated by reversed-phase HPLC at 365 nm using a microBondapak C18 column. The mobile phase was methanol/H2O (70:30, v/v). The assay was linear from 0.25 to 100 microg/mL. Within- and between-day precision and accuracy for this assay were better than 9% and 6%, respectively. The lower limits of detection and quantitation were estimated to be 0.1 and 0.25 microg/mL, respectively. This simple new method has been applied to determine mancozeb concentration in rat urine samples from urinary excretion studies.
Departement of Pharmacology, EA 525, Université Victor Segalen 33076 Bordeaux, France.
Ikram
I
Debbarh
Karine
K
Titier
Evelyne
E
Deridet
Nicholas
N
Moore
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D005659:Fungicides, Industrial / Q000032:analysis* / Q000737:chemistry* / Q000493:pharmacokinetics; D007274:Injections, Intraperitoneal; D008344:Maneb / Q000032:analysis* / Q000737:chemistry* / Q000493:pharmacokinetics; D051381:Rats; D015203:Reproducibility of Results; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D015038:Zineb / Q000032:analysis* / Q000737:chemistry* / Q000493:pharmacokinetics
D005659:Fungicides, Industrial; D013438:Sulfhydryl Compounds; D008344:Maneb; C102217:1,2-benzenedithiol; C013099:mancozeb; D015038:Zineb