Immunohistochemical detection of glucose transporters class I subfamily in the mouse, rat and human testis.
A family of glucose transporters (GLUT) mediates the cellular uptake of glucose at the plasma membrane by facilitated diffusion. We investigated the presence of isoforms GLUT1-4 of class I subfamilies in different types of cells in the mouse, rat and human testis by indirect immunofluorescence technique. Immunocytochemical analyses demonstrated that GLUT1 was expressed in the rat testis, GLUT2 in the mouse and rat testis, GLUT3 in the mouse, rat and human testis and GLUT4 was not presented in the testis at all. A very intensive positive immunoreaction for GLUT3 was found in Sertoli cells, peritubular myoid cells, macrophage-like interstitial cells, testicular endothelial cells and early spermatocytes. GLUT3 positive cells were not found in the luminal part of Sertoli cells, spermatids or Leydig cells. The present results suggest that glucose uptake in different testicular cells is mediated by GLUT1, GLUT2 and GLUT3 and the GLUT3 was the prominent glucose transporter type in the testicular cells.
Department of Anatomy, University of Tartu, Biomedicum, Ravila 19, 50411 Tartu, Estonia. kerstiko@ut.ee
Kersti
K
Kokk
Esko
E
Veräjänkorva
Xiao-Ke
XK
Wu
Helle
H
Tapfer
Elle
E
Põldoja
Pasi
P
Pöllänen
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D005629:Frozen Sections; D005947:Glucose / Q000378:metabolism*; D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D051275:Glucose Transporter Type 4; D006801:Humans; D007150:Immunohistochemistry; D007985:Leydig Cells / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009004:Monosaccharide Transport Proteins / Q000032:analysis* / Q000378:metabolism; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D051381:Rats; D017208:Rats, Wistar; D012708:Sertoli Cells / Q000378:metabolism; D013087:Spermatids / Q000378:metabolism; D013090:Spermatocytes / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D014018:Tissue Distribution
D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D051275:Glucose Transporter Type 4; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; C495677:SLC2A1 protein, human; C495687:SLC2A3 protein, human; C495692:SLC2A4 protein, human; C495678:Slc2a1 protein, mouse; C495679:Slc2a1 protein, rat; C495689:Slc2a3 protein, mouse; C495690:Slc2a3 protein, rat; C495693:Slc2a4 protein, mouse; C495694:Slc2a4 protein, rat; D005947:Glucose