Role of attP in integrase-mediated integration of the Shigella resistance locus pathogenicity island of Shigella flexneri.
The Shigella resistance locus (SRL) pathogenicity island (PAI) in Shigella spp. mediates resistance to streptomycin, ampicillin, chloramphenicol, and tetracycline. It can be excised from the chromosome via site-specific recombination mediated by the P4-related int gene. Here, we show that SRL PAI attP is capable of RecA-independent, site-specific, int-mediated integration into two bacterial tRNA attB sites.
Department of Microbiology, Monash University, Victoria 3800, Australia.
Sally A
SA
Turner
Shelley N
SN
Luck
Harry
H
Sakellaris
Kumar
K
Rajakumar
Ben
B
Adler
D001287:Attachment Sites, Microbiological / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005798:Genes, Bacterial / Q000235:genetics; D019426:Integrases / Q000235:genetics*; D012329:RNA, Bacterial / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D011995:Recombination, Genetic; D012763:Shigella flexneri / Q000187:drug effects* / Q000235:genetics* / Q000472:pathogenicity
D012329:RNA, Bacterial; D012343:RNA, Transfer; D019426:Integrases