Patterning adhesion of mammalian cells with visible light, tris(bipyridyl)ruthenium(II) chloride, and a digital micromirror array.
Patterns of cellular adhesion were created on a surface using novel photochemistry that is stimulated with visible light. A glass surface coated with polyethylene glycol is nonadhesive to a variety of adherent mammalian cell types. Treatment of that surface with a mixture of tris(bipyridyl)ruthenium(II) chloride, ammonium persulfate, and a tryptophan derivative or tryptophan-bearing peptide in conjunction with irradiation with visible light (447 nm) made the surface adhesive to several cell types including mouse fibroblasts, human myoblasts, and human lung tumor cells. Immunostaining data suggest that tryptophan-containing peptides are crosslinked intact to the surface by this chemistry, which enables patterning of peptides containing only naturally occurring amino acids. Microscopic patterns of cellular adhesion were created with this chemistry by projecting microscopic patterns of visible light with a digital micromirror array. Using this method, regions of cellular adhesion were patterned with single-cell resolution.
Luebke
Kevin J
KJ
Center for Biomedical Inventions, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9185, USA. kevin.luebke@utsouthwestern.edu
Carter
Duane E
DE
Garner
Harold R
HR
Brown
Kathlynn C
KC
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D006801:Humans; D008027:Light; D051379:Mice; D041681:NIH 3T3 Cells; D016716:PC12 Cells; D010777:Photochemistry; D051381:Rats; D017975:Ruthenium Compounds / Q000737:chemistry*; D013997:Time Factors
D017975:Ruthenium Compounds