Multitude multicolor chromosome banding (mMCB) - a comprehensive one-step multicolor FISH banding method.
Multicolor chromosome banding (MCB) using one single chromosome-specific MCB probe set per experiment was previously reported as powerful tool in molecular cytogenetics for the characterization of all kinds of human marker chromosomes. However, a quick analysis of karyotypes with highly complex chromosomal changes was hampered by the problem that up to 24 MCB experiments were necessary for a comprehensive karyotype description. To overcome that limitation the 138 available region-specific microdissection-derived libraries for all human chromosomes were combined to one single probe set, called multitude MCB (mMCB). A typical fluorescence banding pattern along the human karyotype is produced, which can be evaluated either by transforming these profiles into chromosome region-specific pseudo-colors or more reliably by studying the fluorescence profiles. The mMCB probe set has been applied on chromosomes of normal male and female probands, two primary myelodysplastic syndromes and two solid tumor cell lines. Additionally, a cell line of Gorilla gorilla (GGO) studied previously by single chromosome-specific MCB was reevaluated by the mMCB method. All results were in concordance with those obtained in parallel or by other cytogenetic and molecular cytogenetic approaches indicating that mMCB is a powerful multicolor FISH banding tool for fast characterization of complex karyotypes.
Weise
A
A
Institute of Human Genetics and Anthropology, University of Jena, Jena, Germany.
Heller
A
A
Starke
H
H
Mrasek
K
K
Kuechler
A
A
Pool-Zobel
B L
BL
Claussen
U
U
Liehr
T
T
D000818:Animals; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D002871:Chromosome Banding / Q000379:methods*; D003116:Color; D005260:Female; D006071:Gorilla gorilla; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D008297:Male; D008677:Metaphase; D009190:Myelodysplastic Syndromes / Q000235:genetics; D009369:Neoplasms / Q000235:genetics