A peptidyl transferase ribozyme capable of combinatorial peptide synthesis.
The formation of peptide bonds is a key step in both the chemical and biological synthesis of peptides. The ribozyme can use a wide range of amino acids as its substrate for the dipeptide synthesis. A library containing 29 peptides whose synthesis was catalyzed by this unique ribozyme was analyzed by mass spectrometry. These results implicate that ribozyme may have potential application in the peptide synthesis.
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Zhiyong
Z
Cui
Lele
L
Sun
Biliang
B
Zhang
D020650:Combinatorial Chemistry Techniques; D004151:Dipeptides / Q000032:analysis / Q000138:chemical synthesis*; D013058:Mass Spectrometry; D019151:Peptide Library; D010458:Peptidyl Transferases / Q000378:metabolism*; D016337:RNA, Catalytic / Q000138:chemical synthesis / Q000378:metabolism*
D004151:Dipeptides; D019151:Peptide Library; D016337:RNA, Catalytic; D010458:Peptidyl Transferases