Capillary electrochromatography of peptides on a neutral porous monolith with annular electroosmotic flow generation.
A new kind of monolithic capillary column was prepared for capillary electrochromatography (CEC) with a positively charged polymer layer on the inner wall of a fused-silica capillary and a neutral monolithic packing as the bulk stationary phase. The fused-silica capillary was first silanized with 3-glycidoxypropyltrimethoxysilane (GPTMS). Polyethyleneimine (PEI) was then covalently bonded to the GPTMS coating to form an annular positively charged polymer layer for the generation of electroosmotic flow (EOF). A neutral bulk monolithic stationary phase was then prepared by in situ copolymerization of vinylbenzyl chloride (VBC) and ethylene glycol dimethacrylate in the presence of 1-propanol and formamide as porogens. Benzyl chloride functionalities on the monolith were subsequently hydrolyzed to benzyl alcohol groups. Effects of pH on the EOF mobility of the column were measured to monitor the completion of reactions. Using a column with this design, we expected general problems in CEC such as irreversible adsorption and electrostatic interaction between stationary phase and analytes to be reduced. A peptide mixture was successfully separated in counter-directional mode CEC. Comparison of peptide separations in isocratic monolithic CEC, gradient HPLC and capillary zone electrophoresis (CZE) indicated that the separation in CEC is governed by a dual mechanism that involves a complex interplay between selective chromatographic retention and differential electrophoretic migration.
Department of Chemical Engineering, Yale University, New Haven, CT 06520-8286, USA.
Yan
Y
Li
Rong
R
Xiang
Csaba
C
Horváth
James A
JA
Wilkins
D002851:Chromatography, High Pressure Liquid; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D010455:Peptides / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D012821:Silanes / Q000737:chemistry
D010455:Peptides; D011108:Polymers; D012821:Silanes