The metNPQ operon of Bacillus subtilis encodes an ABC permease transporting methionine sulfoxide, D- and L-methionine.
The Bacillus subtilis yusCBA operon, which encodes an ABC-type transporter, contains an S-box motif in its promoter region. We showed that the expression of these genes is repressed via the S-box system when methionine is available. The YusCB proteins are involved in the transport of both d- and l-methionine but also methionine sulfoxide. A yusCB mutant is unable to grow in the presence of 5 microM l-methionine or 100 microM methionine sulfoxide, while it grows similarly to the wild type with 100 microM l-methionine and 1 mM methionine sulfoxide. Other uptake systems are therefore present for these two compounds. In contrast, the Yus ABC transporter corresponds to the sole d-methionine uptake system. We propose to rename yusC, yusB and yusA as metN, metP and metQ, respectively.
Unité de Génétique des Génomes Bactériens, Institut Pasteur, URA CNRS 2171, 28, rue du Docteur Roux, 75724 Paris Cedex 15, France.
Marie-Françoise
MF
Hullo
Sandrine
S
Auger
Elie
E
Dassa
Antoine
A
Danchin
Isabelle
I
Martin-Verstraete
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism; D026905:Amino Acid Transport Systems / Q000235:genetics / Q000378:metabolism; D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism; D008715:Methionine / Q000031:analogs & derivatives* / Q000378:metabolism*; D009876:Operon / Q000235:genetics
D018528:ATP-Binding Cassette Transporters; D026905:Amino Acid Transport Systems; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C466430:MetQ protein, E coli; D008715:Methionine; D000251:Adenosine Triphosphatases; C466428:MetN protein, E coli; C013111:methionine sulfoxide