Determination of volatile anesthetics isoflurane and enflurane in mouse brain tissues using gas chromatography-mass spectrometry.
BACKGROUND
A method for determination of the volatile anesthetics, isoflurane, and enflurane in mouse brain tissues using headspace gas chromatography-mass spectrometry (GC-MS) is described.


METHODS
Halothane was used as internal standard (I.S.). Brain samples were completely homogenized in ice-cold water and isoflurane, enflurane, and I.S. were extracted with headspace. One milliliter of headspace gas was injected onto the GC-MS and separation was achieved by using porous layer open tubular (PLOT) capillary column with a solid stationary phase (GSC). As a result, isoflurane, enflurane, and halothane were cleanly separated.


RESULTS
The method demonstrated satisfactory recovery (72% and 76% for isoflurane and enflurane, respectively) and linear calibration ranges of 0.015-2.20 and 0.0152-3.94 microg/sample for isoflurane and enflurane, respectively. Reproducibility calculated as CV% was 3.3-3.9% for all intraday and interday determinations. The procedure was applied for quantitation of isoflurane and enflurane in about 300 mouse brain samples for genetic behavioral study.


CONCLUSIONS
The method was achieved and shown to be effective.
Deng
Xin-Sheng
XS
Department of Pharmacology, University of Colorado Health Sciences Center, Mail Stop C-236, 4200 E. 9th Avenue, Denver, CO 80262, USA. xin-sheng.Deng@uchsc.edu
Simpson
Victoria J
VJ
D018685:Anesthetics, Inhalation / Q000032:analysis* / Q000493:pharmacokinetics; D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D004737:Enflurane / Q000032:analysis* / Q000493:pharmacokinetics; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007530:Isoflurane / Q000032:analysis* / Q000493:pharmacokinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015203:Reproducibility of Results
D018685:Anesthetics, Inhalation; D004737:Enflurane; D007530:Isoflurane