Spectrophotometric method for determining gibberellic acid in fermentation broths.
A novel method for the quantitative determination of gibberellic acid in fermentation broths has been developed. It is based on the kinetic of the reaction of conversion of gibberellic acid to gibberellenic acid. The method is simple, reliable, faster than most of methods known, and free of the interferences which commonly affect spectrophotometric methods currently in use. Its threshold sensitivity is 0.1 g and its accuracy is greater than 97% for concentrations of gibberellic acid ranging from 0.1 to 1 g l(-1).
Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, General Cruz 34, Valparaíso, Chile.
Julio
J
Berríos
Andrés
A
Illanes
Germán
G
Aroca
D003470:Culture Media / Q000032:analysis* / Q000378:metabolism*; D005285:Fermentation / Q000502:physiology; D005874:Gibberella / Q000254:growth & development* / Q000378:metabolism*; D005875:Gibberellins / Q000032:analysis* / Q000096:biosynthesis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods*
D003470:Culture Media; D005875:Gibberellins; C007842:gibberellic acid