[Effects of basic fibroblast growth factor on growth of human periodontal ligament cells].
OBJECTIVE
To evaluate the biological effects of basic fibroblast growth factor (bFGF) on proliferation and DNA synthesis of periodontal ligament cells(PDLC).


METHODS
PDLC was isolated from periodontal ligament explants. The effects of bFGF on PDLC were assayed by MTT and 3(H)-TdR incorporation studies. The results were expressed as A value and CPM. Analysis of variance was used.


RESULTS
bFGF could enhance the proliferation and DNA synthesis of PDLC in a dose dependent manner. No inhibition was observed within 1000ng/ml bFGF concentration. The concentration of half maximal effect was 100ng/ml.


CONCLUSIONS
The results suggest that bFGF may play an important role in the regeneration of periodontal tissue.
Zhang
S J
SJ
Department of Stomatology, Shandong Medical University, Shandong 250012, China. zhangshaojunsd@sina.com
Liang
Y H
YH
Yang
P S
PS