Highly sensitive detection of GG mismatched DNA by surfaces immobilized naphthyridine dimer through poly(ethylene oxide) linkers.
Naphthyridine dimer is a unique molecule that strongly, and selectively, binds to the guanine-guanine mismatch in duplex DNA. We have synthesized naphthyridine dimers possessing a different length of poly(ethylene oxide) (PEO) linker, and immobilized them to CM5 sensor chip to carry out a surface plasmon resonance (SPR) assay of DNA duplexes containing a single base mismatch. The sensitivity of the sensor remarkably increased with increasing numbers of PEO units incorporated into the linker. With the sensor surface immobilized naphthyridine dimer for 1.5 x 10(3) response unit (RU) through three PEO units, the distinct SPR signal was observed at a concentration of 1 nM of the 27-mer G-G mismatch.
Departmrent of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 615-8510, Japan. nakatani@sbchem@kyoto-u.ac.jp
Kazuhiko
K
Nakatani
Akio
A
Kobori
Hiroyuki
H
Kumasawa
Isao
I
Saito
D020137:Base Pair Mismatch; D004247:DNA / Q000737:chemistry; D006147:Guanine / Q000737:chemistry*; D009287:Naphthyridines / Q000737:chemistry*; D009692:Nucleic Acid Heteroduplexes / Q000032:analysis; D011092:Polyethylene Glycols / Q000737:chemistry*; D011485:Protein Binding
D009287:Naphthyridines; D009692:Nucleic Acid Heteroduplexes; D011092:Polyethylene Glycols; D006147:Guanine; D004247:DNA