Platelet function abnormalities in qualified whole-blood donors: effects of medication and recent food intake.
OBJECTIVE
Platelet function abnormalities have been reported in blood donors who have not consumed aspirin. Our objective was to identify factors other than aspirin that may contribute to impaired platelet function in qualified volunteer blood donors.


METHODS
Blood samples were obtained from 24 donors following routine blood donation. Donors completed a study questionnaire that included questions about recent food consumption, medication and medical history. Platelet activation was measured using monoclonal antibodies and flow cytometry. CD62P expression and PAC-1 binding on platelets were used as indicators of platelet activation. Platelet function was measured on a platelet function analyser (PFA-100) using both collagen/epinephrine (cEPI) and collagen/ADP (cADP) cartridges.


RESULTS
Fifty-four per cent of donors (13 of 24) had normal platelet function. Thirty-eight per cent (nine of 24) had prolonged cEPI closure times, of whom four (17%) had no cEPI closure (> 300 seconds). No closure was associated with aspirin use (two donors) or chocolate consumption (two donors) before donation. Two donors (8%) had either a shortened cEPI or cADP closure time.


CONCLUSIONS
Platelet dysfunction in qualified blood donors is underestimated. Platelet function screening can identify donors with diet-related platelet dysfunction or with poor recollection of aspirin use.
Paglieroni
T G
TG
BloodSource-Center for Blood Research, Sacramento, CA, USA University of California Davis Medical Center, Sacramento, CA, USA. teresa.paglieroni@bloodsource.org
Janatpour
K
K
Gosselin
R
R
Crocker
V
V
Dwyre
D M
DM
MacKenzie
M R
MR
Holland
P V
PV
Larkin
E C
EC
D000328:Adult; D000368:Aged; D001241:Aspirin / Q000494:pharmacology; D001782:Blood Donors; D001803:Blood Transfusion / Q000592:standards*; D002099:Cacao / Q000009:adverse effects; D005260:Female; D005502:Food / Q000009:adverse effects; D006801:Humans; D008297:Male; D008875:Middle Aged; D015539:Platelet Activation; D010979:Platelet Function Tests; D011795:Surveys and Questionnaires
D001241:Aspirin