A novel tubular scaffold for cardiovascular tissue engineering.
We have developed a counter rotating cone extrusion device to produce the next generation of three-dimensional collagen scaffold for tissue engineering. The device can produce a continuously varying fibril angle from the lumen to the outside of a 5-mm-diameter collagen tube, similar to the pattern of heart muscle cells in the intact heart. Our scaffold is a novel, oriented, type I collagen, tubular scaffold. We selected collagen because we believe there are important signals from the collagen both geometrically and biochemically that elicit the in vivo -like phenotypic response from the cardiomyocytes. We have shown that cardiomyocytes can be cultured in these tubes and resemble an in vivo phenotype. This new model system will provide important information leading to the design and construction of a functional, biologically based assist device.
Yost
Michael J
MJ
Department of Surgery, University of South Carolina School of Medicine, Columbia, South Carolina 29208, USA. Yost@med.sc.edu
Baicu
Catalin F
CF
Stonerock
Charles E
CE
Goodwin
Richard L
RL
Price
Robert L
RL
Davis
Jeffrey M
JM
Evans
Heather
H
Watson
Phillip D
PD
Gore
C Michael
CM
Sweet
Janea
J
Creech
Laura
L
Zile
Michael R
MR
Terracio
Louis
L
D001672:Biocompatible Materials; D003094:Collagen; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods; D032383:Myocytes, Cardiac / Q000166:cytology; D023822:Tissue Engineering
D001672:Biocompatible Materials; D003094:Collagen