The differentiation of early embryonic stem cells into adipocytes-like cells.
An early embryonic stem cell line, EES-6 cells, was established from 2-cell stage embryos of ddY mice. The cells were maintained in an undifferentiated state with D-MEM/F12 medium supplemented with 10% fetal bovine serum (FBS) (GM) and 1 ng of leukemia inhibitory factor (LIF) without any feeder cells. In this study, EES cells were cultured with a medium containing embryotrophic factors (ETFs) which promoted the differentiation of EES cells into white and brown adipocytes-like cells for a period of 5 days. Lipid droplets in brown adipocyte-like cells were stained with Sudan III; however, large lipid-like droplets in white or brown adipocyte-like cells were unstained with either Sudan III or alcian blue. These findings have numerous possibilities for therapeutic use such as regeneration of skin and wound healing.
Yamamoto
Motoyoshi
M
Dept of Anatomy, The Jikei University School of Medicine, Tokyo, Japan.
Tachibana
Toshiaki
T
Hashimoto
Hisashi
H
Ishiwata
Isamu
I
Ishikawa
Hiroshi
H
D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D003470:Culture Media; D004622:Embryo, Mammalian / Q000166:cytology*; D015850:Interleukin-6 / Q000494:pharmacology; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D051379:Mice; D008815:Mice, Inbred Strains; D018121:Receptors, Cytokine / Q000502:physiology; D053606:Receptors, OSM-LIF; D044968:Regenerative Medicine; D015398:Signal Transduction / Q000187:drug effects; D013234:Stem Cells / Q000166:cytology*
D003470:Culture Media; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; C507838:Lif protein, mouse; C507841:Lifr protein, mouse; D018121:Receptors, Cytokine; D053606:Receptors, OSM-LIF