Role of attP in integrase-mediated integration of the Shigella resistance locus pathogenicity island of Shigella flexneri.
The Shigella resistance locus (SRL) pathogenicity island (PAI) in Shigella spp. mediates resistance to streptomycin, ampicillin, chloramphenicol, and tetracycline. It can be excised from the chromosome via site-specific recombination mediated by the P4-related int gene. Here, we show that SRL PAI attP is capable of RecA-independent, site-specific, int-mediated integration into two bacterial tRNA attB sites.
Turner
Sally A
SA
Department of Microbiology, Monash University, Victoria 3800, Australia.
Luck
Shelley N
SN
Sakellaris
Harry
H
Rajakumar
Kumar
K
Adler
Ben
B
D001287:Attachment Sites, Microbiological / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005798:Genes, Bacterial / Q000235:genetics; D019426:Integrases / Q000235:genetics*; D012329:RNA, Bacterial / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D011995:Recombination, Genetic; D012763:Shigella flexneri / Q000187:drug effects* / Q000235:genetics* / Q000472:pathogenicity
D012329:RNA, Bacterial; D012343:RNA, Transfer; D019426:Integrases