Evaluation of groEL gene analysis for identification of Borrelia burgdorferi sensu lato.
The nucleotide sequences of the groEL genes, the flagellin genes, and the 16S rRNA genes from 22 reference strains of Borrelia were compared. groEL sequence analysis is useful not only in interspecies differentiation but also in intraspecies differentiation of Borrelia afzelii and Borrelia garinii isolates.
Department of Microbiology, College of Medicine, Konkuk University, Chungju, Chungchungbuk-Do 380-701, Korea.
Hyo-Soon
HS
Park
Jung-Hee
JH
Lee
Eun-Ju
EJ
Jeong
Seong-Eun
SE
Koh
Tae-Kyu
TK
Park
Won-Jong
WJ
Jang
Kyung-Hee
KH
Park
Bum-Joon
BJ
Kim
Yoon-Hoh
YH
Kook
Seung-Hyun
SH
Lee
D000818:Animals; D001483:Base Sequence; D001898:Borrelia / Q000145:classification / Q000235:genetics; D025065:Borrelia burgdorferi / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D018834:Chaperonin 60 / Q000235:genetics*; D017931:DNA Primers; D005408:Flagellin / Q000235:genetics; D006801:Humans; D018884:Ixodes / Q000382:microbiology; D008193:Lyme Disease / Q000175:diagnosis; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012867:Skin / Q000382:microbiology; D013045:Species Specificity
D018834:Chaperonin 60; D017931:DNA Primers; D005408:Flagellin