DNA amplification and rearrangements in archival Salmonella enterica serovar Typhimurium LT2 cultures.
Variations in genome size and gene order were observed in archival Salmonella enterica serovar Typhimurium cultures stored for over 40 years. In one strain, microarray analysis revealed a large, stable amplification. PCR analysis of the same strain revealed a genomic duplication that underwent a translocation. Other strains had smaller duplications and deletions. These results demonstrate that storage in stabs over time at room temperature not only allows for further bacterial growth but also may produce an environment that selects for a variety of mutations, including genomic rearrangements.
Porwollik
Steffen
S
Sidney Kimmel Cancer Center, San Diego, California 92121, USA.
Wong
Rita Mei-Yee
RM
Helm
R Allen
RA
Edwards
Kelly K
KK
Calcutt
Michael
M
Eisenstark
Abraham
A
McClelland
Michael
M
D018070:Biological Specimen Banks; D019143:Evolution, Molecular; D017353:Gene Deletion; D020440:Gene Duplication; D014644:Genetic Variation; D016680:Genome, Bacterial; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D012486:Salmonella typhimurium / Q000235:genetics* / Q000254:growth & development*; D014178:Translocation, Genetic; D012407:rRNA Operon