Correlation between invasiveness of colorectal tumor cells and adhesive potential under flow.
BACKGROUND
Tumor cell adhesiveness is involved in metastatic dissemination, and adhesive behavior may be different under static and dynamic conditions.


METHODS
Patients undergoing primary colorectal cancer excision were tested for: i) serum concentration of sE-selectin, sICAM-1 and sVCAM-1, ii) expression of CD18, CD29d and E-cadherin on tumor cells and iii) efficiency of tumor cell adhesion to ECV304 monolayers under flow and resistance to detachment by shear.


RESULTS
Twenty out of 31 patients were free of detectable relapse 12 months later. Relapsing and non-relapsing patients had similar levels of soluble adhesion molecules. E-cadherin was detected on tumor cells from three non-relapsing patients, but no relapsing one. Unexpectedly, significant CD18 labeling was found on two relapsing patients and one non-relapsing patient. Cells from relapsing patients displayed significantly increased (p < 0.05 two-sided, p < 0.025 one-sided) capacity to adhere to test monolayers under flow.


CONCLUSIONS
Cancer invasion is related to tumor cell adhesiveness, and the flow chamber provides a practical way of measuring adhesive parameters with a potential value for relapse prediction.
Benoliel
Anne-Marie
AM
Laboratoire d'Immunologie, INSERM U387/CNRS FRE2059, Hôpital de Sainte-Marguerite, BP 29, 13274 Marseille, France.
Pirro
Nicolas
N
Marin
Valérie
V
Consentino
Bernard
B
Pierres
Anne
A
Vitte
Joana
J
Bongrand
Pierre
P
Sielezneff
Igor
I
Sastre
Bernard
B
D015703:Antigens, CD / Q000032:analysis; D015415:Biomarkers / Q000097:blood; D018821:CD18 Antigens / Q000032:analysis; D015820:Cadherins / Q000032:analysis; D002448:Cell Adhesion / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000097:blood*; D003110:Colonic Neoplasms / Q000097:blood / Q000473:pathology* / Q000503:physiopathology* / Q000601:surgery; D019040:E-Selectin / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D019012:Integrin beta1 / Q000032:analysis; D009361:Neoplasm Invasiveness / Q000473:pathology*; D012004:Rectal Neoplasms / Q000097:blood / Q000473:pathology* / Q000503:physiopathology* / Q000601:surgery; D013314:Stress, Mechanical; D019010:Vascular Cell Adhesion Molecule-1 / Q000097:blood
D015703:Antigens, CD; D015415:Biomarkers; D018821:CD18 Antigens; D015820:Cadherins; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D019012:Integrin beta1; D019010:Vascular Cell Adhesion Molecule-1