Cloning and expression of cytosolic phosphoglycerate kinase from pea (Pisum sativum L.).
In common with several other respiratory and photosynthetic enzymes, a sub-population of cytosolic phosphoglycerate kinase (PGK) occurs in the nucleus in pea leaves and shoots. The full-length cDNA encoding pea cytosolic PGK has been cloned and sequenced, revealing not only the PGK 'signature' but also a nuclear localization signal (NLS). A translational fusion of PGK and GFP was used to transform tobacco BY-2 cells resulting in GFP locating to the cell nuclei.
Department of Biological Sciences, University of Exeter, Exeter EX4 4PS, UK.
David C
DC
Brice
John A
JA
Bryant
Gerardas
G
Dambrauskas
Suzanne C
SC
Drury
Jennifer A
JA
Littlechild
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003600:Cytosol / Q000201:enzymology; D017931:DNA Primers; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000201:enzymology* / Q000235:genetics*; D010735:Phosphoglycerate Kinase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D010735:Phosphoglycerate Kinase