Enhanced productivity of G1 phase Chinese hamster ovary cells using the GADD153 promoter.
Productivity of three different promoters at various cell cycle stages and under two distinct growth conditions was examined in Chinese hamster ovary cells. Under the Growth Arrest and DNA Damage inducible GADD153 promoter, productivity of the short half-live variant of the enhanced green fluorescent protein (d2EGFP) and the secreted alkaline phosphatase (SEAP) was highest at the G1 phase of the cell cycle and at serum starvation, while under the cytomegalovirus (CMV) or the simian virus SV40 promoter, productivity was highest at S-phase and in complete medium. These results indicate the utility of the GADD153 promoter for production purposes under protein-free conditions.
School of Biotechnology and Biomolecular Sciences, University of NSW Sydney NSW 2052, Australia.
Leonore
L
de Boer
Peter P
PP
Gray
Noelle-Ann
NA
Sunstrom
D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D016466:CHO Cells / Q000166:cytology* / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods; D006224:Cricetinae; D003412:Cricetulus; D016895:Culture Media, Serum-Free; D016193:G1 Phase / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology; D024861:Genetic Enhancement / Q000379:methods*; D011401:Promoter Regions, Genetic; D015202:Protein Engineering / Q000379:methods*; D051742:Transcription Factor CHOP; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
D022762:CCAAT-Enhancer-Binding Proteins; D016895:Culture Media, Serum-Free; D014157:Transcription Factors; D051742:Transcription Factor CHOP; D000469:Alkaline Phosphatase