[Morphodynamic analysis of the role of leukocytes in the microcirculation disorder of the cerebral cortex].
The aim of this work was to establish the reasons for the disturbances of microcirculation (no reflow) during various kinds of oxygen starvation of the brain. Using contact optics and video-recording, direct observations were performed and leukocyte and erythrocyte displacement was monitored in the blood vessels of rat cerebral cortex in vivo in normoxia, during the development of various kinds of oxygen deficiency up to the terminal periods of life of an organism. In normoxia leukocytes were shown to decelerate erythrocyte movement in capillaries and to cause the irregularity of the capillary blood flow. Using the color videofilm recording it was shown that at deep stages of brain oxygen deficiency the disturbances of microcirculation up to its complete cessation were explained by the occlusion of venous microvessels as a result of massive adhesion of leukocytes to the inner surface of venules and the smallest veins. The co-adhesion of leukocytes was also found to take place. The latter leads to the formation of large leukocyte aggregates in venous vessels, results in "no reflow" effect. Leukocyte aggregates in veins block the venous outflow from the brain and may become the immediate cause of organism death as a result of disruption of circulation and oxygen deficiency.
Group of Thermoregulation and Bioenergetics, RAS I.P. Pavlov Institute of Physiology, St. Petersburg.
K P
KP
Ivanov
N N
NN
Mel'nikova
D000818:Animals; D002196:Capillaries / Q000473:pathology; D002448:Cell Adhesion / Q000502:physiology; D002540:Cerebral Cortex / Q000098:blood supply*; D003326:Coronary Circulation / Q000502:physiology*; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000473:pathology; D000860:Hypoxia / Q000097:blood / Q000473:pathology / Q000503:physiopathology; D007962:Leukocytes / Q000502:physiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014699:Venules / Q000473:pathology