A LC/MS method for the determination of cyanobacteria toxins in water.
The cyanobacteria toxins anatoxin-a, microcystin-LR, microcystin-RR, microcystin-YR, and nodularin were separated in less than 30 min on several 1 mm x 15 cm reversed-phase liquid chromatography (LC) columns, and their electrospray mass spectra were measured using injections of 50 ng or less with a benchtop time-of-flight (TOF) mass spectrometer. New data from this work include the following: (a) the impact of acetic acid concentrations in the methanol-water mobile phase on measured ion abundances; (b) the performance of the electrospray-TOF mass spectrometer as an LC detector; (c) the accuracy and precision of exact m/z measurements after LC separation with a routinely used mass spectrometer resolving power of 5000; and (d) recoveries of the five toxins from reagent water, river waters, and sewage treatment plant effluent samples extracted with C-18 silica particles enmeshed in thin Teflon membrane filter disks. This technique has the potential of providing a relatively simple and reasonable-cost sample preparation and LC/MS method that provides the sensitivity, selectivity, reliability, and information content needed for source and drinking water occurrence and human exposure studies.
Maizels
Mila
M
Oak Ridge Institute for Science and Education, 26 West Martin L. King Jr. Drive, Cincinnati, Ohio 45268, USA.
Budde
William L
WL
D019342:Acetic Acid; D001427:Bacterial Toxins / Q000032:analysis* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D000087522:Cyanobacteria Toxins; D003500:Cyclization; D008387:Marine Toxins / Q000032:analysis* / Q000737:chemistry; D000432:Methanol; D052998:Microcystins; D015394:Molecular Structure; D012107:Research Design; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D014326:Tropanes; D014867:Water / Q000737:chemistry*
D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D008387:Marine Toxins; D052998:Microcystins; D014326:Tropanes; D014867:Water; C509783:anatoxin a; D019342:Acetic Acid; D000432:Methanol