[Effects of selenium on rat hepatocellular DNA damage induced by cadmium in vitro].
OBJECTIVE
This study was conducted to explore effects of selenium on rat hepatocellular DNA damage induced by cadmium in vitro.


METHODS
Sodium selenite was added at concentrations of 8.75, 17.50 and 35.00 micromol/L respectively with cadmium chloride at the concentrations of 8.75, 17.50 and 35.00 micromol/L respectively and rat hepatocellular DNA damage was measured with single cell gel electrophoresis (comet assay).


RESULTS
Sodium selenite at the concentration of 8.75 micromol/L inhibited DNA damage caused by cadmium chloride at the concentration of 8.75, 17.50 and 35.00 micromol/L in rat liver cells (P < 0.05). Although sodium selenite at 17.50 micromol/L inhibited DNA damage induced by cadmium chloride at 17.50 and 35.00 micromol/L, it did not inhibit DNA damage induced by cadmium chloride at 8.75 micromol/L. Sodium selenite at 35.00 micromol/L did not have antagonistic effects on DNA damage induced by cadmium chloride at 8.75, 17.50 and 35.00 micromol/L. In addition, sodium selenite at 8.75 micromol/L had the best antagonistic effect while cadmium chloride at 8.75 micromol/L, but the antagonistic effect of sodium selenite at 17.50 micromol/L was better than 8.75 micromol/L while cadmium chloride at 17.50 and 35.00 micromol/L.


CONCLUSIONS
The antagonistic effect of selenium on rat hepatocellular DNA damage induced by cadmium related to the concentrations of selenium and also to the concentration ratio between selenium and cadmium.
Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Ri-an
RA
Yu
Xue-min
XM
Chen
D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D020552:Comet Assay; D004247:DNA / Q000187:drug effects / Q000235:genetics; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D012643:Selenium / Q000494:pharmacology*
D002104:Cadmium; D004247:DNA; D012643:Selenium