[Effect of purified pre-beta 1 HDL on efflux of 3H-cholesterol loaded in human smooth muscle cells].
OBJECTIVE
To compare the effects of different HDL subclasses on the efflux of 3H-cholesterol loaded human smooth muscle cells (SMCS).


METHODS
3H-cholesterol loaded human SMCS were incubated with purified pre-beta 1 HDL, alpha-HDL and apoE-deficient HDL3 respectively. The cholesterol effluxing capacity and incubating time of different HDL subclasses were detected, and were analysed by double-reciprocal mapping.


RESULTS
It was found that the value of Emax of pre-beta 1 HDL is higher than that of alpha-HDL and apoE-deficient HDL3 (P < 0.01), and the value of Ke of pre-beta 1 HDL is lower than that of alpha-HDL and apoE-deficient HDL3 (P < 0.01).


CONCLUSIONS
The results showed the effluxing capacity and efficiency of pre-beta 1 HDL were greater than those of alpha-HDL and apoE-deficient HDL3, thus suggesting that pre-beta 1 HDL is a more efficient acceptor of cell-derived 3H-cholesterol.
Wu
Xin-wei
XW
Apolipoprotein Research Laboratory, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Yang
Lu-chuan
LC
Xu
Yan-hua
YH
Fu
Qiang
Q
Fu
Ming-de
MD
D001011:Aorta / Q000166:cytology; D001057:Apolipoproteins E / Q000378:metabolism; D001692:Biological Transport; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism*; D008076:Cholesterol, HDL / Q000494:pharmacology; D053442:High-Density Lipoproteins, Pre-beta; D006801:Humans; D008075:Lipoproteins, HDL / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*
D001057:Apolipoproteins E; D008076:Cholesterol, HDL; D053442:High-Density Lipoproteins, Pre-beta; D008075:Lipoproteins, HDL; D002784:Cholesterol