[Impact of simulated microgravity on the expression and distribution of cardiac gap junction protein CX43].
OBJECTIVE
To observe the expression and distribution changes of connexin 43 (CX) in rats' myocardium after simulated microgravity and explore the partial mechanism of arrhythmia.


METHODS
Male Wistar rats were randomly assigned to either tail-suspension group (SUS) or control group (CON). Immunohistochemistry and Western Blot were used to detect the expression and the distribution of CX43. Electromicroscope was used to observe the ultrastructural changes.


RESULTS
In SUS group, the decrease of CX43 and the distribution disturbance were obvious (P<0.05), the proportion of the side-to-side gap junction increased (P<0.05), and space between some gap junctions disappeared.


CONCLUSIONS
The results show that CX43 decreased significantly and distributed irregularly after simulated microgravity. These can cause the changes of cardiac electric conduction velocity and direction. As a result, conduction block and reentry may occur. And these might be the partial mechanism of cardiac arrhythmia.
Institute of Space Medico-Engineering, Beijing, China.
Zhao-xia
ZX
Liu
Tie-min
TM
Ma
Hong-hui
HH
Yang
Da-wei
DW
Wu
De-sheng
DS
Wang
Shu-jing
SJ
Zhang
D000818:Animals; D001145:Arrhythmias, Cardiac / Q000209:etiology; D018031:Connexin 43 / Q000378:metabolism*; D017629:Gap Junctions / Q000378:metabolism* / Q000648:ultrastructure; D019417:Hindlimb Suspension / Q000009:adverse effects*; D007150:Immunohistochemistry; D008297:Male; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure*; D032383:Myocytes, Cardiac / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D018474:Weightlessness Simulation / Q000009:adverse effects*
D018031:Connexin 43