A high-resolution comparative RH map of the telomeric end of bovine chromosome 2 with human chromosomes 1 and 2.
High density livestock to human comparative maps are necessary for the implementation of comparative positional candidate gene cloning. We have constructed a high-density comparative radiation hybrid (RH) map of the telomeric end of bovine chromosome 2 (BTA2) using a 12,000-rad whole genome cattle-hamster radiation hybrid (WGRH) panel. Eighteen bovine EST markers with orthologues on human chromosomes 1 and 2 (HSA1 and HSA2), together with nine microsatellite markers, were typed against the 180 cell lines of the WGRH panel. Twenty-one markers were included in the multi-point framework map at LOD =3.0. The comparative analysis reveals a new segment of highly conserved synteny between HSA2 and BTA2.
Department of Animal Science, University of Missouri-Columbia, Columbia, Missouri, USA.
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Liu
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JE
Womack
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Antoniou
D000818:Animals; D002417:Cattle / Q000235:genetics*; D002878:Chromosomes, Human, Pair 1; D002889:Chromosomes, Human, Pair 2; D033481:Chromosomes, Mammalian; D006224:Cricetinae; D006801:Humans; D051379:Mice; D018895:Microsatellite Repeats; D021101:Radiation Hybrid Mapping; D026801:Synteny; D016615:Telomere