Electrophoresis time impacts the denaturing gradient gel electrophoresis-based assessment of bacterial community structure.
We investigated the impact of denaturing gradient gel electrophoresis (DGGE) run time on the assessment of bacterial community structure. Results indicated that increased electrophoresis run time (while maintaining 1000 volt-hours) resulted in dissimilar profiles, likely due to instability of the denaturing gradient. We recommend that DGGE run times be minimized to provide optimal band resolution, as extended electrophoresis times can greatly impact subsequent band-based analyses.
Sigler
W V
WV
Department of Earth, Ecological and Environmental Sciences, University of Toledo, 2801 W. Bancroft MS 604, Toledo, OH 43606, USA. von.sigler@utoledo.edu
Miniaci
C
C
Zeyer
J
J
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D012988:Soil Microbiology; D013997:Time Factors