Characterization of transcriptional oscillation of an Arabidopsis homolog of PnC401 related to photoperiodic induction of flowering in Pharbitis nil.
AtC401 is an Arabidopsis homolog of PnC401 that is related to photoperiodic induction of flowering in Pharbitis nil. These genes show free-running rhythms. To study the free-running rhythm of AtC401, we fused a firefly luciferase reporter to the AtC401 promoter and transformed it into Arabidopsis plants. The observed bioluminescence oscillated under continuous light and continuous dark only with sucrose supplementation. The free-running period of bioluminescence was temperature-compensated between 22 degrees C and 30 degrees C. Light-pulse experiments under continuous darkness produced a phase-response curve typical of circadian rhythms. We conclude that rhythmic expression of AtC401 is controlled by a circadian oscillator.
Gene Research Center, Institute of Biological Sciences, University of Tsukuba, Tsukuba, 305-8572 Japan. oguchit@sakura.cc.tsukuba.ac.jp
Taichi
T
Oguchi
Kimiyo
K
Sage-Ono
Hiroshi
H
Kamada
Michiyuki
M
Ono
D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics*; D001683:Biological Clocks / Q000235:genetics*; D002940:Circadian Rhythm / Q000235:genetics; D035264:Flowers / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D005809:Genes, Regulator / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D027741:Ipomoea / Q000235:genetics*; D008156:Luciferases / Q000235:genetics; D008163:Luminescent Measurements; D010775:Photic Stimulation; D017440:Photoperiod; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects
D029681:Arabidopsis Proteins; D008156:Luciferases