[Indentification of differentially expressed genes in proliferating and involuting hemangiomas by cDAN microarray analysis of gene-expression profiles].
OBJECTIVE
To investigate the differentially expressed genes of proliferating and involuting hemangiomas by cDNA microarray analysis of gene-expression profiles in an effort to identify the key disease-related genes.


METHODS
Samples were processed from total RNA and purified to mRNA, which was reverse-transcripted and hybridized onto Biodoor Genechip expression microarrays. Analyses were performed to determine the consensus pattern of gene expression in the proliferating and involuting stages of the same hemangioma and the changes in the expression level.


RESULTS
In proliferating hemangioma, 79 genes were overexpressed, and 115 genes were underexpressed in comparison with the involuting hemangioma. Some cytokines and growth factors such as neurotensin, Nov, CYR6, keratinocyte growth factor, interleukin-10 were overexpressed in proliferative hemangioma. In involuting hemangioma, apoptotic factors such as bcl-2 binding component, cytochrome C were overexpressed. The overexpression of Nov, CYR6, c-myc implied that angiogenesis and oncogenes might participate in the pathogenesis of hemangiomas. Mitochondria activated apoptotic passage (cytokines, bcl-2, cytochrome C) and Wnt/beta-catenin passage(Frizzled, beta-catenin, c-myc) were involved.


CONCLUSIONS
The development of hemangiomas may be the results of imbalance of cell proliferation and apoptosis.
Zhang
Li
L
Department of Plastic and Reconstructive Surgery, Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China.
Wang
Wei
W
Qi
Zuo-liang
ZL
Dong
Jia-sheng
JS
Song
Huai-dong
HD
Yang
Jin-song
JS
Lin
Xiao-xi
XX
D017209:Apoptosis; D002455:Cell Division; D005260:Female; D020869:Gene Expression Profiling; D006391:Hemangioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis