Differential expression of Desulfovibrio vulgaris genes in response to Cu(II) and Hg(II) toxicity.
The response of Desulfovibrio vulgaris to Cu(II) and Hg(II) was characterized. Both metals increased the lag phase, and Cu(II) reduced cell yield at concentrations as low as 50 microM. mRNA expression was analyzed using random arbitrarily primed PCR, differential display, and quantitative PCR. Both Cu(II) and Hg(II) (50 micro M) caused upregulation of mRNA expression for an ATP binding protein (ORF2004) and an ATPase (ORF856) with four- to sixfold increases for Hg(II) and 1.4- to 3-fold increases with Cu(II). These results suggest that D. vulgaris uses an ATP-dependent mechanism for adapting to toxic metals in the environment.
Chang
In Seop
IS
Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019-0245, USA.
Groh
Jennifer L
JL
Ramsey
Matthew M
MM
Ballard
Jimmy D
JD
Krumholz
Lee R
LR
D001483:Base Sequence; D003300:Copper / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics; D016969:Desulfovibrio vulgaris / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D005798:Genes, Bacterial; D008628:Mercury / Q000494:pharmacology*; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019105:Random Amplified Polymorphic DNA Technique
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012333:RNA, Messenger; D003300:Copper; D008628:Mercury