A simple method for studying whole sections of rice grain.
We developed a new and simple method to collect sections of a whole brown rice kernel for investigation of histological properties. A single kernel of rice was dehydrated through a graded ethanol series, transferred to xylene, and embedded in paraffin. During sectioning of the blocks using a rotary microtome, we used a special adhesive tape to collect and place the sections on slides so they remained flat. The use of the adhesive tape technique combined with autofluorescence characteristics allowed us to visualize cell walls throughout an entire longitudinal or transverse section of a whole rice kernel. We obtained scanning electron microscopy images of the sections to determine section quality.
USDA, ARS, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA.
Y
Y
Ogawa
W J
WJ
Orts
G M
GM
Glenn
D F
DF
Wood
D002523:Edible Grain / Q000166:cytology; D016591:Histocytological Preparation Techniques / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D012275:Oryza / Q000737:chemistry / Q000166:cytology*; D012639:Seeds / Q000166:cytology / Q000648:ultrastructure