A novel DNA-based microfluorimetric method to evaluate antimalarial drug activity.
This paper describes the development of a novel microfluorimetric assay to measure the inhibition of Plasmodium falciparum based on the detection of parasitic DNA by intercalation with PicoGreen. The method was used to determine parasite inhibition profiles and 50% inhibitory concentration values of known or potential antimalarial drugs. Values for parasite inhibition with known anti-malarial drugs using the PicoGreen assay were comparable with those determined by the standard method based upon the uptake of 3H-hypoxanthine and the Giemsa stain microscopic technique. The PicoGreen assay is rapid, sensitive, reproducible, easily interpreted, and ideally suited for screening of large numbers of samples for anti-malarial drug development.
Instituto de Investigaciones Científicas Avanzadas y Servicios de Alta Tecnología, Ciudad del Saber, Clayton, Panama.
Yolanda
Y
Corbett
Liuris
L
Herrera
Jose
J
Gonzalez
Luis
L
Cubilla
Todd L
TL
Capson
Phyllis D
PD
Coley
Thomas A
TA
Kursar
Luz I
LI
Romero
Eduardo
E
Ortega-Barria
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D003592:Cytophotometry; D016054:DNA, Protozoan / Q000032:analysis*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000469:parasitology; D005456:Fluorescent Dyes; D006801:Humans; D009930:Organic Chemicals; D021261:Parasitic Sensitivity Tests / Q000379:methods*; D010936:Plant Extracts / Q000494:pharmacology; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D015203:Reproducibility of Results; D012588:Scintillation Counting; D012680:Sensitivity and Specificity
D000962:Antimalarials; D016054:DNA, Protozoan; D005456:Fluorescent Dyes; D009930:Organic Chemicals; C099476:PicoGreen; D010936:Plant Extracts