Nonlinear decrease of background fluorescence in polymer thin-films - a survey of materials and how they can complicate fluorescence detection in microTAS.
Polymers and plastics are receiving increased attention as materials for microfluidics and microTAS applications. Given the ubiquity of fluorescence detection techniques in micro-analytical systems, the fluorescence properties of polymers and plastics should not be overlooked. We survey some commonly available polymer thin-films for their fluorescence behaviour under standardized conditions to determine which materials are most suitable for high-sensitivity fluorescence detection lab chips. The initial fluorescence intensities of some of the materials surveyed were significantly higher than glass and fused silica controls, and decreased over the three hour period with complex kinetics. We then discuss how this has confounded fluorescence detection in our analytical context, and possible mechanisms for the decrease.
Hawkins
Kenneth R
KR
Department of Bioengineering, University of Washington, Seattle, Washington, USA. khawk@u.washington.edu
Yager
Paul
P
D008567:Membranes, Artificial; D008856:Microscopy, Fluorescence; D036103:Nanotechnology; D017711:Nonlinear Dynamics; D011093:Polyethylene Terephthalates / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D008567:Membranes, Artificial; D011093:Polyethylene Terephthalates; D011108:Polymers; C025539:Lavsan