Expression of ornithine decarboxylase of Coccidioides immitis in three Escherichia coli strains carrying the lambda DE3 lysogen and an E. coli EWH319 strain odc- null mutant.
Ornithine decarboxylase from respiratory fungal pathogen, Coccidioides immitis, cloned in the pETCiODC plasmid under control of T7lac promoter, was produced in E. coli BL21(DE3), BL21(DE3)pLysS, BLR(DE3) and EWH319 transformant strains. E. coli BL21(DE3)pLysS-pETCiODC expressed the highest specific activity of ODC, suggesting that this strain could be successfully used for protein structure and drug testing studies.
Pantoja-Hernández
Miguel Angel
MA
Instituto Tecnológico de Celaya, Departamento de Ingenieráa Bioquímica, Ave. Tecnológico v A. Garcia-Cubas, S/N, Colonia FOVISSSTE, Apartado postal 57, Celaya, Guanajuato, México.
Muñoz-Sánchez
Claudia Ivonne
CI
Guevara-González
Ramón Gerardo
RG
Botello-Alvarez
Enrique
E
González-Chavira
Mario Martín
MM
Torres-Pacheco
Irineo
I
Guevara-Olvera
Lorenzo
L
D003045:Coccidioides / Q000201:enzymology* / Q000235:genetics*; D004789:Enzyme Activation; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D009955:Ornithine Decarboxylase / Q000096:biosynthesis* / Q000235:genetics*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis; D013045:Species Specificity; D013379:Substrate Specificity; D014169:Transformation, Bacterial
D011994:Recombinant Proteins; D009955:Ornithine Decarboxylase