Relief of catabolite repression in a cAMP-independent catabolite gene activator mutant of Escherichia coli.
We isolated and characterized a new catabolite gene activator mutant (crp*) of Escherichia coli that confers cAMP-independent expression and total relief of catabolite repression of beta-galactosidase and tryptophanase synthesis. The two mutations responsible for this phenotype change the amino acids at codon 72 from Glu to Ala and at codon 144 from Ala to Thr in the corresponding CAP* protein.
Karimova
Gouzel
G
Department of Structural Biology and Chemistry, CNRS URA 2185, Institut Pasteur, 28, rue du Docteur Roux, 75724 Paris Cedex 15, France.
Ladant
Daniel
D
Ullmann
Agnes
A
D000242:Cyclic AMP / Q000378:metabolism*; D002373:Cyclic AMP Receptor Protein / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D020868:Gene Silencing; D009154:Mutation; D001616:beta-Galactosidase / Q000096:biosynthesis
D002373:Cyclic AMP Receptor Protein; D000242:Cyclic AMP; D001616:beta-Galactosidase