The effect of the linker on the hydrolysis rate of drug-linked ester bonds.
Tailoring the length of a sulfide containing linker adjusts the hydrolysis of a drug-linked ester bond to values appropriate for once-a-week administrations. A model drug of paclitaxel was coupled using a hydrolyzable linker to a poly(ethylene glycol) macromonomer, via a conjugate addition reaction between a thiol and an acrylamide. The macromonomers were synthesized in three steps with an average overall yield of 70%. By changing the length of the linker from 3-sulfanylpropionyl to 4-sulfanylbutyryl, the half-life time of the release of the drug could be increased from 4.2+/-0.1 to 14.0+/-0.2 days. Drug-containing hydrogels were prepared by radical photopolymerization of these macromonomers with either the 3-sulfanylpropionyl or the 4-sulfanylbutyryl linker. The release of the drug from these hydrogels followed similar trends as the release of the drug from the soluble polymer-drug conjugates. The synthetic methodology employed does not involve the use of coupling reagents in the final conjugation between the drug and the polymer, excluding the presence of potential toxic residuals. The conjugation method is relatively simple and is applicable to nearly any hydroxyl-containing drugs.
Institute for Biomedical Engineering, ETH and University of Zurich, Moussonstrasse 18, CH-8044 Zurich, Switzerland. ronald.schoenmakers@mat.ethz.ch
Ronald G
RG
Schoenmakers
Petra
P
van de Wetering
Donald L
DL
Elbert
Jeffrey A
JA
Hubbell
D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004952:Esters / Q000737:chemistry*; D005782:Gels; D020100:Hydrogels; D006868:Hydrolysis; D006900:Hydroxylation; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles; D008958:Models, Molecular; D017239:Paclitaxel / Q000008:administration & dosage / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D014677:Pharmaceutical Vehicles; D011092:Polyethylene Glycols; D012996:Solutions; D013440:Sulfides
D000972:Antineoplastic Agents, Phytogenic; D003432:Cross-Linking Reagents; D004952:Esters; D005782:Gels; D020100:Hydrogels; D007202:Indicators and Reagents; D008823:Micelles; D004364:Pharmaceutical Preparations; D014677:Pharmaceutical Vehicles; D012996:Solutions; D013440:Sulfides; D011092:Polyethylene Glycols; D017239:Paclitaxel