Ultrastructural effects of two phthalocyanines in CHO-K1 and HeLa cells after laser irradiation.
The effects of Photodynamic Therapy using 2nd generation photosensitizers have been widely investigated aiming clinical application treatment of solid neoplasms. In this work, ultrastructure changes caused by the action of two 2nd generation photosensitizers and laser irradiation on CHO-K1 and HeLa (neoplastic) cells were analyzed by transmission electron microscopy. Aluminum phthalocyanine chloride, aluminum phthalocyanine tetrasulfonate chloride and radiation from a semiconductor laser at a fluency of 0.5 J/cm2 (Power=26 mW; lambda=.670 nm) were used. The results showed induction of apoptosis. Such alterations where observed in HeLa but not in CHO-K1 cells after Aluminum phthalocyanine tetrasulfonate chloride (AlPcS4, photodynamic treatment. The Aluminum phthalocyanine chloride (AlPc) photodynamic treatment induced necrosis on the neoplastic cell line, and cytoplasm and nuclear alterations on the normal cell line.
Laboratório de Biologia Celular e Tecidual, Instituto de Pesquisa e Desenvolvimento, Universidade do Vale do Paraíba, Av. Shishima Hifumi, 2911, CEP 12244-000, São José dos Campos, São Paulo, Brazil.
Marcelo
M
de Castro Pazos
Cristina
C
Pacheco-Soares
Newton
N
Soares da Silva
Renato Augusto
RA
DaMatta
Marcos Tadeu T
MT
Pacheco
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D016466:CHO Cells; D002467:Cell Nucleus / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D006224:Cricetinae; D003593:Cytoplasm / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D005260:Female; D006367:HeLa Cells; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D007834:Lasers; D008027:Light; D008854:Microscopy, Electron; D008928:Mitochondria / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D009336:Necrosis; D009942:Organometallic Compounds / Q000494:pharmacology; D010053:Ovary / Q000187:drug effects* / Q000648:ultrastructure; D010775:Photic Stimulation / Q000295:instrumentation / Q000379:methods; D010778:Photochemotherapy / Q000379:methods*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000648:ultrastructure
D007211:Indoles; D009942:Organometallic Compounds; D011838:Radiation-Sensitizing Agents; C048577:aluminum tetrasulfophthalocyanine; C044797:chloroaluminum phthalocyanine