Regulation of lantibiotic lacticin 481 production at the transcriptional level by acid pH.
The lantibiotic lacticin 481 operon (lctAMTFEG) is mainly transcribed from P1 and P3, two promoters lying upstream of lctA. A weak additional promoter allows independent expression of the immunity genes (lctFEG). Lacticin 481 production by Lactococcus lactis is stimulated by the acidification due to lactic acid production, and by artificially lowering the pH of the medium. This regulation occurs at the transcriptional level, since P1 and P3 are both acid-induced. P1 is weaker but more tightly regulated than P3. As no specific regulator is encoded by the lacticin 481 operon, P1 and P3 are likely controlled by a general regulator.
Laboratoire de Biologie et Chimie Moléculaires, EA 2594, Université de Bretagne Sud, P.O. Box 92116, 56321 Lorient, France.
Thomas
T
Hindré
Jean-Paul
JP
Le Pennec
Dominique
D
Haras
Alain
A
Dufour
D000143:Acids / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics*; D001430:Bacteriocins / Q000235:genetics*; D004274:DNA, Recombinant; D015964:Gene Expression Regulation, Bacterial; D006863:Hydrogen-Ion Concentration; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism; D009876:Operon; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D000143:Acids; D001426:Bacterial Proteins; D001430:Bacteriocins; D004274:DNA, Recombinant; C082508:lacticin 481