Prion protein is a component of the multimolecular signaling complex involved in T cell activation.
In this study we analyzed the interaction of prion protein PrP(C) with components of glycosphingolipid-enriched microdomains in lymphoblastoid T cells. PrP(C) was distributed in small clusters on the plasma membrane, as revealed by immunoelectron microscopy. PrP(C) is present in microdomains, since it coimmunoprecipitates with GM3 and the raft marker GM1. A strict association between PrP(C) and Fyn was revealed by scanning confocal microscopy and coimmunoprecipitation experiments. The phosphorylation protein ZAP-70 was immunoprecipitated by anti-PrP after T cell activation. These results demonstrate that PrP(C) interacts with ZAP-70, suggesting that PrP(C) is a component of the multimolecular signaling complex within microdomains involved in T cell activation.
Dipartimento di Medicina Sperimentale e Patologia, Università 'La Sapienza', viale Regina Elena 324, 00161 Rome, Italy.
Vincenzo
V
Mattei
Tina
T
Garofalo
Roberta
R
Misasi
Annapia
A
Circella
Valeria
V
Manganelli
Giuseppe
G
Lucania
Antonio
A
Pavan
Maurizio
M
Sorice
D048868:Adaptor Proteins, Signal Transducing; D000911:Antibodies, Monoclonal / Q000378:metabolism; D018106:CD28 Antigens / Q000378:metabolism; D017252:CD3 Complex / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D005677:G(M1) Ganglioside / Q000378:metabolism; D005679:G(M3) Ganglioside / Q000378:metabolism; D006801:Humans; D008213:Lymphocyte Activation; D021962:Membrane Microdomains / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D018613:Microscopy, Confocal; D018621:PrPC Proteins / Q000378:metabolism* / Q000648:ultrastructure; D011233:Precipitin Tests; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology*; D051746:ZAP-70 Protein-Tyrosine Kinase
D048868:Adaptor Proteins, Signal Transducing; D000911:Antibodies, Monoclonal; D018106:CD28 Antigens; D017252:CD3 Complex; D002352:Carrier Proteins; C106957:FYB1 protein, human; D005679:G(M3) Ganglioside; D018621:PrPC Proteins; D005677:G(M1) Ganglioside; D011505:Protein-Tyrosine Kinases; D051746:ZAP-70 Protein-Tyrosine Kinase; C497261:ZAP70 protein, human