Simultaneous detection of seven mutations with seven forward primers and one common reverse primer in a single PCR step.
A new PCR method is described here to amplify simultaneously several fragments sharing the same template in a single PCR step with a hemi-primer. Using this method, we successfully detected various point mutations in tissue plasminogen activator (tPA) mutants.
Biotechnology Research Center and Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences, Zhongshan University, Canton 510275, PR China.
Qiang
Q
Wang
Qiuyun
Q
Liu
Bao-jian
BJ
Li
D004252:DNA Mutational Analysis; D017931:DNA Primers / Q000737:chemistry*; D018076:DNA, Complementary / Q000378:metabolism; D005821:Genetic Techniques; D006801:Humans; D009154:Mutation; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D010959:Tissue Plasminogen Activator / Q000235:genetics
D017931:DNA Primers; D018076:DNA, Complementary; D010959:Tissue Plasminogen Activator