Isoflavones inhibit 3-hydroxy-3-methylglutaryl coenzyme A reductase in vitro.
Isoflavones identified as inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase in soybean paste were assayed using the catalytic portion of Syrian hamster HMG-CoA reductase, and the kinetic values were measured using HMG-CoA and NADPH. The inhibition of HMG-CoA reductase by these inhibitors was competitive with HMG-CoA and noncompetitive with NADPH. Ki values for genistein, daidzein, and glycitein were 27.7, 49.5, and 94.7 microM, respectively.
Sung
Jang Hoon
JH
Department of Food Science and Technology, School of Agricultural Biotechnology, and Center for Agricultural Biomaterials, Seoul National University, Korea.
Lee
Sung-Joon
SJ
Park
Kwan Hwa
KH
Moon
Tae Wha
TW
D000465:Algorithms; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D019833:Genistein / Q000494:pharmacology; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics / Q000378:metabolism*; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D007529:Isoflavones / Q000737:chemistry / Q000494:pharmacology*; D007700:Kinetics; D013058:Mass Spectrometry; D008647:Mesocricetus; D009249:NADP / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D013025:Glycine max / Q000737:chemistry
D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D007529:Isoflavones; D009249:NADP; C004742:daidzein; C086566:glycitein; D019833:Genistein; D006903:Hydroxymethylglutaryl CoA Reductases