Direct monitoring of enzymatic glucan hydrolysis on a 27-MHz quartz-crystal microbalance.
By using a highly sensitive 27-MHz glucan-immobilized quartz-crystal microbalance, we could follow kinetically all processes (enzyme binding and release, kon and koff, and intramolecular hydrolysis rates, kcat) of glucan hydrolysis by glucoamylase by detecting directly the formation and decay of the enzyme-substrate complex as mass changes.
Nishino
Hidekazu
H
Department of Biomolecular Engineering, Tokyo Institute of Technology and CREST, JST, Nagatsuda, Midori-ku, Yokohama 226-8501, Japan.
Nihira
Takanori
T
Mori
Toshiaki
T
Okahata
Yoshio
Y
D000687:Amylopectin / Q000737:chemistry* / Q000378:metabolism; D001234:Aspergillus niger / Q000201:enzymology; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry* / Q000378:metabolism; D005936:Glucans / Q000737:chemistry* / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D008832:Microchemistry / Q000379:methods
D004800:Enzymes, Immobilized; D005936:Glucans; C009109:pullulan; D000687:Amylopectin; D005087:Glucan 1,4-alpha-Glucosidase