PRDI-BF1 recruits the histone H3 methyltransferase G9a in transcriptional silencing.
PRDI-BF1, the human ortholog of mouse Blimp-1, is a DNA-binding protein involved in postinduction repression of interferon-beta gene transcription in response to viral infection. PRDI-BF1 also has an essential function in driving terminal differentiation of B lymphocytes and therein silences multiple genes. Here we show PRDI-BF1 assembles silent chromatin over the interferon-beta promoter in the osteosarcoma cell line U2OS through recruitment of the histone H3 lysine methyltransferase G9a. G9a is recruited only when in a complex with PRDI-BF1. G9a catalytic activity is required for the accumulation of methylated histone H3 and transcriptional silencing mediated by PRDI-BF1 in vivo. This establishes a mechanism for the recruitment of G9a, the main mammalian euchromatic methyltransferase, and defines nonembryonic targets of G9a.
Gyory
Ildikó
I
Department of Biochemistry and Molecular Biology, H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, 12902 Magnolia Drive, MRC4East, Tampa, FL 33612, USA.
Wu
Jian
J
Fejér
György
G
Seto
Edward
E
Wright
Kenneth L
KL
D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D002843:Chromatin / Q000235:genetics; D020868:Gene Silencing; D000076983:Histone Methyltransferases; D011495:Histone-Lysine N-Methyltransferase; D006801:Humans; D016899:Interferon-beta / Q000235:genetics; D046911:Macromolecular Substances; D008780:Methyltransferases / Q000378:metabolism*; D008969:Molecular Sequence Data; D000074462:Positive Regulatory Domain I-Binding Factor 1; D011496:Protein Methyltransferases; D021381:Protein Transport; D012097:Repressor Proteins / Q000502:physiology*; D013379:Substrate Specificity; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic
D002843:Chromatin; D046911:Macromolecular Substances; C513258:Prdm1 protein, mouse; D012097:Repressor Proteins; D014157:Transcription Factors; C087042:PRDM1 protein, human; D016899:Interferon-beta; D000076983:Histone Methyltransferases; D008780:Methyltransferases; D000074462:Positive Regulatory Domain I-Binding Factor 1; D011496:Protein Methyltransferases; D011495:Histone-Lysine N-Methyltransferase