Resistant starch modulates in vivo colonic butyrate uptake and its oxidation in rats with dextran sulfate sodium-induced colitis.
We previously demonstrated improvements of colonic lesions due to dextran sulfate sodium (DSS) in rats after 7 d of supplementation with resistant starch (RS) type 3, a substrate yielding high levels of butyrate (C(4)), a colonic cell fuel source. In the present study, we hypothesized that if inflammation is related to decreased C(4) utilization by the colonic mucosa, RS supplementation should restore C(4) use simultaneously with an increase in the amount of C(4) present in the digestive tract. Hence, we compared, in vivo, the cecocolonic uptake of C(4) and its oxidation into CO(2) and ketone bodies in control and DSS-treated rats fed a fiber-free basal diet (BD) or a RS-supplemented diet. Sprague-Dawley rats (n = 60) were used. DSS treatment was performed to induce acute colitis and then to maintain chronic colitis. After cecal infusion of [1-(13)C]-C(4) (20 micro mol in 1 h), concentrations and (13)C-enrichment of C(4), ketone bodies, and CO(2) were quantified in the abdominal aorta and portal vein. Portal blood flow was recorded. During acute colitis, (13)C(4) uptake and (13)CO(2) production were lower in DSS rats than in controls. During chronic colitis, DSS rats did not differ from controls. After 7 d of chronic colitis, RS-DSS rats exhibited the same C(4) uptake as BD-DSS rats in spite of higher C(4) cecocolonic disposal. After 14 d, C(4) uptake was higher in RS-DSS than in BD-DSS rats. Thus, the increased utilization of C(4) by the mucosa is subsequent to evidence of healing and appears to be a consequence rather than a cause of this RS healing effect.
Moreau
Noëlle M
NM
Unité de Nutrition et d'Endocrinologie, Ecole Nationale Vétérinaire, Nantes, France.
Champ
Martine M
MM
Goupry
Stéphane M
SM
Le Bizec
Bruno J
BJ
Krempf
Michel
M
Nguyen
Patrick G
PG
Dumon
Henri J
HJ
Martin
Lucile J
LJ
D000208:Acute Disease; D000818:Animals; D002087:Butyrates / Q000378:metabolism*; D002245:Carbon Dioxide / Q000097:blood; D002247:Carbon Isotopes; D002432:Cecum / Q000378:metabolism; D002908:Chronic Disease; D003092:Colitis / Q000139:chemically induced* / Q000378:metabolism; D003106:Colon / Q000378:metabolism*; D016264:Dextran Sulfate / Q000494:pharmacology*; D004063:Digestion / Q000502:physiology; D004327:Drinking Behavior; D005247:Feeding Behavior; D005772:Gastrointestinal Transit / Q000502:physiology*; D007413:Intestinal Mucosa / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods; D007657:Ketone Bodies / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013213:Starch / Q000494:pharmacology*; D015430:Weight Gain / Q000502:physiology
D002087:Butyrates; D002247:Carbon Isotopes; D007657:Ketone Bodies; D002245:Carbon Dioxide; D013213:Starch; D016264:Dextran Sulfate