Stretch-mediated responses of osteoblast-like cells cultured on titanium-coated substrates in vitro.
Cyclic stretching experiments on osteoblast-like cells have proven to be a useful tool in understanding the underlying mechanisms of load transduction at the bone-implant surface. However, most experimental setups use silicone rubber substrates, which are atypical for orthopedic and dental implant materials. Therefore, we investigated the responses of osteoblast-like cells to loading on titanium (Ti)-coated versus plain silicone substrates. Ti-coated substrates were made by a radio-frequency magnetron sputtering process, and characterized using Rutherford backscattering spectrometry, X-ray photoelectron spectroscopy, and contact-angle measurements. Osteoblast-like cells cultured from rat bone marrow were seeded on both types of substrates and stretched for 1 h continuously. Subsequently, cell proliferation, alkaline phosphatase activity, and calcium content were measured for up to 24 days after seeding. In addition light-, scanning electron-, and confocal laser scanning micrographs were made. The results showed that our Ti coating had a thickness of 50 nm and contained Ti/oxygen as 1:1. However, further characterization proved that the silicone material had a tendency to resurface through the coating. Osteoblast-like cells proliferated faster on the Ti-coated substrates, but differentiation was slower compared with the silicone substrates. It was concluded that that there was a definitive influence of the substrate material in mechanical stress models. Therefore, extrapolation of results obtained using silicone substrates cannot be translated directly toward the situation of metallic implant materials.
Department of Biomaterials, University Medical Center, College of Dental Science, PO Box 9101, 6500 HB Nijmegen, The Netherlands.
X F
XF
Walboomers
W J E M
WJ
Habraken
B
B
Feddes
L C
LC
Winter
J D
JD
Bumgardner
J A
JA
Jansen
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D004577:Electron Probe Microanalysis; D005269:Femur / Q000166:cytology*; D008297:Male; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D012826:Silicone Elastomers / Q000494:pharmacology*; D017154:Stromal Cells / Q000502:physiology; D014025:Titanium / Q000494:pharmacology*
D020099:Coated Materials, Biocompatible; D012826:Silicone Elastomers; D004247:DNA; D014025:Titanium; D000469:Alkaline Phosphatase