MYRbase: analysis of genome-wide glycine myristoylation enlarges the functional spectrum of eukaryotic myristoylated proteins.
We evaluated the evolutionary conservation of glycine myristoylation within eukaryotic sequences. Our large-scale cross-genome analyses, available as MYRbase, show that the functional spectrum of myristoylated proteins is currently largely underestimated. We give experimental evidence for in vitro myristoylation of selected predictions. Furthermore, we classify five membrane-attachment factors that occur most frequently in combination with, or even replacing, myristoyl anchors, as some protein family examples show.
IMP Research Institute of Molecular Pathology, Dr, Bohr-Gasse 7, A-1030 Vienna, Austria. stroh@imp.univie.ac.at
Sebastian
S
Maurer-Stroh
Masaki
M
Gouda
Maria
M
Novatchkova
Alexander
A
Schleiffer
Georg
G
Schneider
Fernanda L
FL
Sirota
Michael
M
Wildpaner
Nobuhiro
N
Hayashi
Frank
F
Eisenhaber
D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000502:physiology; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000502:physiology; D017124:Conserved Sequence / Q000235:genetics; D030562:Databases, Protein; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins / Q000737:chemistry / Q000502:physiology; D019143:Evolution, Molecular; D016678:Genome; D016681:Genome, Fungal; D015894:Genome, Human; D005998:Glycine / Q000378:metabolism*; D006801:Humans; D051379:Mice; D019814:Myristic Acid / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry* / Q000502:physiology*; D012440:Saccharomyces / Q000235:genetics
D029681:Arabidopsis Proteins; D029742:Caenorhabditis elegans Proteins; D029721:Drosophila Proteins; D011506:Proteins; D019814:Myristic Acid; D005998:Glycine