Cytogenetic mapping and orientation of the rhesus macaque MHC.
Applying fluorescence in situ hybridisation (FISH), six cosmid clones of rhesus macaque origin containing the genes SACM2L, RING1, BAT1 and MIC2, MIC3, MICD, and MOG of the major histocompatibility complex (MHC) were localised to the long arm of the rhesus macaque chromosome 6 in 6q24, the orthologous region to human 6p21.3. Furthermore, centromere to telomere orientation of the rhesus macaque MHC as well as the internal order of the MHC genes tested are the same as in human. Fiber-FISH allows a rough estimate of distances between these MHC genes in the rhesus macaque, and, as in the human, the rhesus macaque MHC comprises about 3 to 4 Mb.
Institut für Humangenetik und Anthropologie, Freiburg, Germany.
I
I
Huber
L
L
Walter
R
R
Wimmer
J J
JJ
Pasantes
E
E
Günther
W
W
Schempp
D000818:Animals; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D033481:Chromosomes, Mammalian / Q000648:ultrastructure; D020732:Cytogenetic Analysis; D023061:Gene Order; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008253:Macaca mulatta / Q000235:genetics*; D008285:Major Histocompatibility Complex; D008677:Metaphase; D026801:Synteny