[Determination of ginkgolic acids in Ginkgo biloba extract and its preparations by high performance liquid chromatography].
OBJECTIVE
To establish a high performance liquid chromatographic method for determination of ginkgolic acids in Ginkgo biloba extract and its preparations.


METHODS
Ginkgo biloba extract and its preparations were extracted with petroleum ether in Soxhlet apparatus, and then concentrated under vacuum. The ginkgolic acids were determined directly by HPLC, and identified by LC/DAD/ESI/MS. The chromatographic column was Inertsil ODS-2; the mobile phase was methanol-3% aqueous acetic acid(92:8); the flow rate was 1.0 mL.min-1; the column temperature was 40 degrees C; the detection wavelength was 310 nm.


RESULTS
There were six kinds of ginkgolic acid (C13:0, C15:1, C17:2, C15:0, C17:1 and an unknown compound C17:3 tentatively) in the Ginkgo biloba extract. The relative percentage content of ginkgolic acids C13:0, C15:1 and C17:1 was above 94%. The content of ginkgolic acids in Ginkgo biloba extract containing high content ginkgolic acids was 1.12%, and RSD was 2.4% (n = 5). The content of ginkgolic acids in one kind of EGb preparations (tablet) was 49.2 micrograms.g-1, and RSD was 4.3% (n = 5). The average recovery was 98.2%, RSD was 2.6% (n = 5).


CONCLUSIONS
The method is accurate, fast, simple, and can be used for determination of ginkgolic acids in Ginkgo biloba extract and its preparations.
Wu
Xiang-yang
XY
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China. yangliuqing@ujs.edu.cn
Yang
Liu-qing
LQ
Chen
Jun
J
D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D020441:Ginkgo biloba / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D012459:Salicylates / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization
D004365:Drugs, Chinese Herbal; D012459:Salicylates; C112485:ginkgolic acid