[Experimental study of the inducing BMSCs into osteogenesis].
OBJECTIVE
To study the bone tissue osteogenesis ability of bone marrow stromal cells (BMSCs) in vitro and in vivo.


METHODS
The BMSCs were separated by gradient centrifugation on Percoll, culture-expanded and induced with a conditional medium. The cells were examined by transmission electron microscopy and histochemistry or immunochemistry stains technique including AKP,Cbfa1,OCN, OPN, BSP and collagen type I. The mRNA of collagen type I in cultured cell was also examined by in situ hybridization.


RESULTS
Histochemical studies showed positive stain of AKP,Cbfa1, OCN, OPN,BSP and collagen type I,and in situ hybridization also showed the positive express of mRNA of collagen type I. The excretion of collagen and deposition of calcium salt were observed under transmission electron microscopy.


CONCLUSIONS
BMSCs cultured in conditional medium shows osteoblast-like functions in vitro, which provides an ideal seed cells for bone tissue engineering.
Department of Plastic Surgery, Research Centre of Tissue Engineering Laboratory, Shanghai 200011, China.
Min
M
Wang
Yu-lai
YL
Weng
Xiao-jie
XJ
Hu
Lei
L
Cui
Wei
W
Liu
Xi-ping
XP
Feng
Yi-lin
YL
Cao