Consequences of noise- or styrene-induced cochlear damages on glutamate decarboxylase levels in the rat inferior colliculus.
Both noise and styrene can injure the cochlea, resulting in a reduction of incoming inputs from the cochlea to the central nervous system. In addition, styrene is known to have neurotoxic properties at high doses. The loss of inputs caused by noise has been shown to be compensated by a new equilibrium between excitatory and inhibitory influences within the inferior colliculus (IC). The main goal of this study was to determine whether styrene-induced hearing loss could also be counterbalanced by a GABAergic adjustment in the IC. For this purpose, rats were exposed to noise (97 dB SPL octave band noise centered at 8 kHz), or to a non-neurotoxic dose of styrene for 4 weeks (700 ppm, 6 h/day, 5 days/week). Auditory sensitivity was tested by evoked potentials, and cochlear damage was assessed by hair cell counts. Glutamate decarboxylase (GAD) was dosed in the IC by indirect competitive enzyme-linked immunosorbent assay. Both noise and styrene caused PTSs that reached 27.0 and 14.6 dB respectively. Outer hair cell (OHC) loss caused by noise did not exceed 9% in the first row, on the other hand OHC loss induced by styrene reached 63% in the third row. Only the noise caused a decrease of GAD of 37% compared to that measured in the controls. No significant modification of GAD concentration has been shown after styrene exposure. Thus, central compensation for cochlear damage may depend on the nature of the ototoxic agent. Unless styrene directly affects IC function, it is reasonable to assume that noise causes a modification of inhibitory neurotransmission within the structure because of impairment of afferent supply to the auditory brainstem. The present findings suggest that central compensation for cochlear damage can preferably occur when afferent fibers are altered.
Pouyatos
Benoît
B
Institut National de Recherche et de Sécurité, Laboratoire de Neurotoxicologie, Avenue de Bourgogne, BP 27, 54501 Vandoeuvre, France. benoit.pouyatos@med.va.gov
Morel
Georges
G
Lambert-Xolin
Anne-Marie
AM
Maguin
Katy
K
Campo
Pierre
P
D000818:Animals; D001299:Audiometry; D001309:Auditory Threshold; D003051:Cochlea / Q000187:drug effects* / Q000293:injuries* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005072:Evoked Potentials, Auditory; D005968:Glutamate Decarboxylase / Q000378:metabolism*; D006198:Hair Cells, Auditory / Q000473:pathology; D034381:Hearing Loss / Q000139:chemically induced* / Q000175:diagnosis / Q000201:enzymology / Q000473:pathology; D006317:Hearing Loss, Noise-Induced / Q000175:diagnosis / Q000503:physiopathology; D006320:Hearing Tests; D007245:Inferior Colliculi / Q000201:enzymology*; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D009622:Noise; D051381:Rats; D020318:Rats, Long-Evans; D020058:Styrene / Q000494:pharmacology*; D014947:Wounds and Injuries / Q000201:enzymology* / Q000473:pathology
D007527:Isoenzymes; D020058:Styrene; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1