Upregulation of the pro-opiomelanocortin gene in motoneurones after acrylamide administration in mice.
Immunocytochemistry was used to demonstrate the presence of beta-endorphin and alpha-MSH, and in situ hybridisation was used to study the presence of pro-opiomelanocortin (POMC) mRNA, in spinal motoneurones, up to 8 days following the administration of a single dose of acrylamide in mice. The proportions of POMC-mRNA positive neurones, beta-endorphin-immunoreactive neurones and alpha-MSH-immunoreactive neurones were significantly increased in the treated animals compared to controls. It seems likely that upregulation of the POMC gene precedes acrylamide-induced neuropathy.
Department of Physiology, Medical School, Division of Medical Science, University of Birmingham, Birmingham B15 2TT, UK.
Sharon
S
Hughes
Timothy
T
Child
Michael G
MG
Simpson
Margaret E
ME
Smith
D020106:Acrylamide / Q000008:administration & dosage* / Q000494:pharmacology; D000818:Animals; D002452:Cell Count / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects*; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D008297:Male; D051379:Mice; D009046:Motor Neurons / Q000187:drug effects* / Q000378:metabolism; D011333:Pro-Opiomelanocortin / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects; D013997:Time Factors; D015854:Up-Regulation; D000521:alpha-MSH / Q000378:metabolism; D001615:beta-Endorphin / Q000378:metabolism
D012333:RNA, Messenger; D020106:Acrylamide; D000521:alpha-MSH; D001615:beta-Endorphin; D011333:Pro-Opiomelanocortin