Differential display proteome analysis of PC-12 cells transiently transfected with metallothionein-3 gene.
Metallothionein-3 (MT-3), also known as growth inhibitory factor, possesses several unique properties other than the common features of metallothionein family. To investigate the mechanisms underlying its multifaceted roles in the central nervous system, we employed differential display proteomics techniques to study holistic protein changes of PC-12 cells induced by transient transfection of MT-3. Ten significantly and reproducibly changed proteins were identified and their functional implications are discussed in some detail.
Zhou
Bo
B
Proteome Group, National Laboratory of Protein Engineering, Peking University, Beijing 100871, PRC.
Yang
Wei
W
Ji
Jian-Guo
JG
Ru
Bing-Gen
BG
D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects*; D000090023:Metallothionein 3; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D016716:PC12 Cells; D011506:Proteins / Q000032:analysis / Q000235:genetics; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D051381:Rats; D014162:Transfection
D000090023:Metallothionein 3; D009419:Nerve Tissue Proteins; D011506:Proteins; D020543:Proteome