CD109 represents a novel branch of the alpha2-macroglobulin/complement gene family.
We report here the genomic organization and phylogenic relationships of CD109, a member of the the alpha2-macroglobulin/complement (AMCOM) gene family. CD109 is a GPI-linked glycoprotein expressed on endothelial cells, platelets, activated T-cells, and a wide variety of tumors. We cloned full-length CD109 cDNA from the mammalian U373 cell line by RT-PCR and performed analysis of its corresponding genomic sequence. The CD109 cDNA spans 128 kb of chromosome 6q with its 33 exons constituting approximately 3.3% of the total CD109 genomic sequence. Sequence analysis revealed that CD109 contains specific motifs in its N-terminus, that are highly conserved in all AMCOM members. CD109 also shares motifs with certain other AMCOM members including: (1) a thioester 'GCGEQ" motif, (2) a furin site of four positively charged amino acids, and (3) a double tyrosine near the C-terminus. Based on a phylogenic analysis of human CD109 with other human homologs as well as orthologs from other mammalian species, C. elegans (ZK337.1) and E. coli homologs, we propose CD109 represents a novel and independent branch of the alpha2-macroglobulin/complement gene family (AMCOM) and may be its oldest member.
Department of Orthopaedic Surgery, Children's Hospital, Boston, MA 02115, USA.
Keith R
KR
Solomon
Parul
P
Sharma
Melvin
M
Chan
Paul T
PT
Morrison
Robert W
RW
Finberg
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016466:CHO Cells; D045744:Cell Line, Tumor; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003165:Complement System Proteins / Q000235:genetics*; D006224:Cricetinae; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D019143:Evolution, Molecular; D005091:Exons; D058851:GPI-Linked Proteins; D005796:Genes / Q000235:genetics; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D009363:Neoplasm Proteins; D043265:Phosphatidylinositol Diacylglycerol-Lyase / Q000378:metabolism; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D000511:alpha-Macroglobulins / Q000235:genetics*
D015703:Antigens, CD; C120523:CD109 protein, human; D018076:DNA, Complementary; D058851:GPI-Linked Proteins; D009363:Neoplasm Proteins; D000511:alpha-Macroglobulins; D003165:Complement System Proteins; D043265:Phosphatidylinositol Diacylglycerol-Lyase