Nonylphenol enhances apoptosis induced by serum deprivation in PC12 cells.
Although nonylphenol is well known as an endocrine disrupting chemical, there is little information concerning biological effect of nonylphenol. In this study, we investigated effect of nonylphenol on apoptosis induced by serum deprivation in PC12 cells using TUNEL and DNA fragmentation assays. In addition, changes in contents of proapoptotic factors, Bad and Bax, and antiapoptotic factor, Bcl-2, and enzyme activity of caspase-3 were studied. Below 100 ng/ml of nonylphenol increased TUNEL signals, DNA fragmentation and content of proapoptotic factor, Bad as compared to those by serum deprivation without nonylphenol. Furthermore, addition of nonylphenol enhanced caspase-3 activity and Z-VAD, caspase-3 inhibitor, diminished such effect. These results indicated that below 100 ng/ml of nonylphenol enhanced apoptosis induced by serum deprivation via caspase-3 activation in PC12 cell.
Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Kita-10, Nishi-5, Kita, Sapporo 060-0810, Japan.
Miho
M
Aoki
Masaaki
M
Kurasaki
Takeshi
T
Saito
Sayaka
S
Seki
Toshiyuki
T
Hosokawa
Yasumitsu
Y
Takahashi
Hiroyoshi
H
Fujita
Toshio
T
Iwakuma
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D016895:Culture Media, Serum-Free; D053938:DNA Fragmentation; D004587:Electrophoresis, Agar Gel; D020287:In Situ Nick-End Labeling; D016716:PC12 Cells; D010636:Phenols / Q000378:metabolism / Q000633:toxicity*; D051381:Rats
D016895:Culture Media, Serum-Free; D010636:Phenols; C025256:nonylphenol; D020169:Caspases