Functional analyses of placental protein 13/galectin-13.
Placental protein 13 (PP13) was cloned from human term placenta. As sequence analyses, alignments and computational modelling showed its conserved structural and functional homology to members of the galectin family, the protein was designated galectin-13. Similar to human eosinophil Charcot-Leyden crystal protein/galectin-10 but not other galectins, its weak lysophospholipase activity was confirmed by 31P-NMR. In this study, recombinant PP13/galectin-13 was expressed and specific monoclonal antibody to PP13 was developed. Endogenous lysophospholipase activity of both the purified and also the recombinant protein was verified. Sugar binding assays revealed that N-acetyl-lactosamine, mannose and N-acetyl-glucosamine residues widely expressed in human placenta had the strongest binding affinity to both the purified and recombinant PP13/galectin-13, which also effectively agglutinated erythrocytes. The protein was found to be a homodimer of 16 kDa subunits linked together by disulphide bonds, a phenomenon differing from the noncovalent dimerization of previously known prototype galectins. Furthermore, reducing agents were shown to decrease its sugar binding activity and abolish its haemagglutination. Phosphorylation sites were computed on PP13/galectin-13, and phosphorylation of the purified protein was confirmed. Using affinity chromatography, PAGE, MALDI-TOF MS and post source decay, annexin II and beta/gamma actin were identified as proteins specifically bound to PP13/galectin-13 in placenta and fetal hepatic cells. Perinuclear staining of the syncytiotrophoblasts showed its expression in these cells, while strong labelling of the syncytiotrophoblasts' brush border membrane confirmed its galectin-like externalization to the cell surface. Knowing its colocalization and specific binding to annexin II, PP13/galectin-13 was assumed to be secreted to the outer cell surface by ectocytosis, in microvesicles containing actin and annexin II. With regard to our functional and immunomorphological results, PP13/galectin-13 may have special haemostatic and immunobiological functions at the lining of the common feto-maternal blood-spaces or developmental role in the placenta.
First Department of Obstetrics and Gynaecology, Semmelweis University, Budapest, Hungary. gabor.than@aok.pte.hu
Nandor G
NG
Than
Elah
E
Pick
Szabolcs
S
Bellyei
Andras
A
Szigeti
Ora
O
Burger
Zoltan
Z
Berente
Tamas
T
Janaky
Arpad
A
Boronkai
Harvey
H
Kliman
Hamutal
H
Meiri
Hans
H
Bohn
Gabor N
GN
Than
Balazs
B
Sumegi
D000595:Amino Acid Sequence; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D002460:Cell Line; D019281:Dimerization; D037161:Galectins; D006801:Humans; D037102:Lectins / Q000378:metabolism; D008024:Ligands; D008099:Liver / Q000166:cytology; D008245:Lysophospholipase / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics; D010766:Phosphorylation; D010920:Placenta / Q000378:metabolism; D011257:Pregnancy Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D002241:Carbohydrates; D037161:Galectins; C038195:LGALS13 protein, human; D037102:Lectins; D008024:Ligands; D010446:Peptide Fragments; D011257:Pregnancy Proteins; D011994:Recombinant Proteins; D008245:Lysophospholipase