[Use of methyl parathion (MP)-degrading strain DLL-E4 (Pseudomonas sp.) to remove MP residue on the surface of agricultural products].
In this paper, derivations from DLL-E4, including fermented liquid, its centrifuged (3000 x g, 10 min) supernatant, pellet extract with supersonic, crude enzyme(supernatant and pellet extract), detergent, and mixture of detergent and pellet extract, were used to degrade the MP residue on the surface of Chinese cabbage, tea and cucumber. The results showed that all of them were effective, with the best effect of 100%. Some constituents in the supernatant of fermented liquid could stimulate the enzymatic activity. For tea, the mixture of detergent and enzyme was more effective, but the opposite was found for the other two crops. In all treatments, crude enzyme and pellet extract had the best effect, and the rational added amount of enzyme was 2%, 5% and 10% for cucumber, Chinese cabbage and tea, respectively. Crude enzyme also could degrade methamidophos phoxim and chlorpyrifos.
Liu
Zhi
Z
Microbiology Department, Nanjing Agricultural University, Nanjing, 210095. liuzhi@263.net
Zhang
Xiaozhou
X
Li
Shunpeng
S
D001673:Biodegradation, Environmental; D001937:Brassica / Q000737:chemistry; D018553:Cucumis sativus / Q000737:chemistry; D005285:Fermentation; D005506:Food Contamination; D008743:Methyl Parathion / Q000302:isolation & purification / Q000378:metabolism*; D010573:Pesticide Residues / Q000302:isolation & purification*; D011549:Pseudomonas / Q000378:metabolism*; D013662:Tea / Q000737:chemistry
D010573:Pesticide Residues; D013662:Tea; D008743:Methyl Parathion