Micro- and nanofluidics for DNA analysis.
Miniaturization to the micrometer and nanometer scale opens up the possibility to probe biology on a length scale where fundamental biological processes take place, such as the epigenetic and genetic control of single cells. To study single cells the necessary devices need to be integrated on a single chip; and, to access the relevant length scales, the devices need to be designed with feature sizes of a few nanometers up to several micrometers. We will give a few examples from the literature and from our own research in the field of miniaturized chip-based devices for DNA analysis, including dielectrophoresis for purification of DNA, artificial gel structures for rapid DNA separation, and nanofluidic channels for direct visualization of single DNA molecules.
Department of Molecular Biology, Princeton University, Princeton, NJ, USA. jonas.tegenfeldt@ftf.lth.se
Jonas O
JO
Tegenfeldt
Christelle
C
Prinz
Han
H
Cao
Richard L
RL
Huang
Robert H
RH
Austin
Stephen Y
SY
Chou
Edward C
EC
Cox
James C
JC
Sturm
D002469:Cell Separation; D004247:DNA / Q000032:analysis* / Q000235:genetics; D044085:Microfluidics; D036103:Nanotechnology
D004247:DNA