Improved genetic transformation of the thermophilic cyanobacterium, Thermosynechococcus elongatus BP-1.
We improved genetic transformation of the thermophilic cyanobacterium, Thermosynechococcus elongatus BP-1, by combining electroporation with a top agar method. Transformation was also improved when a disruptant of a putative type I restriction endonuclease (tll2230) was used as recipient cells. In particular, some constructs, with which wild type has never been transformed, were successfully integrated into the tll2230-disruptant. Single-crossover recombination was detected more frequently than the double-crossover recombination. In accordance with the presence of all the homologs of pil genes in Synechocystis sp. PCC 6803, we found that T. elongatus is naturally transformable with exogenous DNA.
Iwai
Masako
M
Department of Life Sciences (Biology), The University of Tokyo, Komaba 3-8-1, Meguro, Tokyo, 153-8902 Japan.
Katoh
Hiroshi
H
Katayama
Mitsunori
M
Ikeuchi
Masahiko
M
D000362:Agar / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D000458:Cyanobacteria / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000494:pharmacology; D018274:Electroporation / Q000379:methods; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D008967:Molecular Biology / Q000379:methods*; D011995:Recombination, Genetic / Q000235:genetics; D015183:Restriction Mapping / Q000379:methods; D014170:Transformation, Genetic / Q000235:genetics*
D000362:Agar; D004247:DNA