Bioconversion of waste office paper to L(+)-lactic acid by the filamentous fungus Rhizopus oryzae.
L(+)-lactic acid production was investigated using an enzymatic hydrolysate of waste office automation (OA) paper in a culture of the filamentous fungus Rhizopus oryzae. In 4 d culture, 82.8 g/l glucose, 7 g/l xylose, and 3.4 g/l cellobiose contained in the hydrolysate were consumed to produce 49.1 g/l of lactic acid. The lactic acid yield and production rate were only 0.59 g/g and 16.3 g/l/d, respectively, only 75% and 61% of the results from the glucose medium. The low production rate from waste OA hydrolysate was elucidated by trials using xylose as the sole carbon source; in those trials, the lactic acid production rate was 7.3 g/l/d, only 28% that of glucose or cellobiose. The low lactic acid yield from waste OA hydrolysate was clarified by trials using artificial hydrolysates comprised of 7:2:1 or 7:1:2 ratios of glucose:cellobiose:xylose. For both, the lactic acid production rate of 17.4 g/l/d matched that of waste OA paper, while the lactic acid yield was similar to that of the glucose medium. This indicates that the production rate may be inhibited by xylose derived from hemicellulose, and the yield may be inhibited by unknown compounds derived from paper pulp.
Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan. yspark@agr.shizuoka.ac.jp
Enoch Y
EY
Park
Pham Ngoc
PN
Anh
Naoyuki
N
Okuda
D000164:Acremonium / Q000201:enzymology; D002475:Cellobiose / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005947:Glucose / Q000378:metabolism; D007564:Japan; D019344:Lactic Acid / Q000096:biosynthesis*; D010209:Paper; D012037:Refuse Disposal / Q000379:methods*; D012233:Rhizopus / Q000378:metabolism*; D013997:Time Factors; D014994:Xylose / Q000378:metabolism
D002475:Cellobiose; D019344:Lactic Acid; D014994:Xylose; D002480:Cellulase; D005947:Glucose