Retinoic acid enhances the gene expression of human polymeric immunoglobulin receptor (pIgR) by TNF-alpha.
In this study, the detailed mechanisms for the effects of vitamin A on the expression of polymeric immunoglobulin receptor (pIgR) were examined. Expression of the pIgR by tumour necrosis factor (TNF-alpha) was enhanced by the addition of all-trans retinoic acid (ATRA) or 9-cis retinoic acid (9CRA). This enhancement was mediated mainly by RARalpha, and regulated at the transcriptional level. Transcription factor nuclear factor-kappaB (NF-kappaB) binding and activation were not influenced by addition of ATRA. These data imply that RA, in combination with TNF-alpha, could up-regulate the expression of pIgR. In addition, we hypothesize that up-regulation of pIgR by RA is controlled through the RAR-dependent signalling pathway and that it plays a role in enhancement of mucosal immunity.
Department of Pathology, Nihon University, School of Dentistry, Tokyo, Japan. takenouchi@dent.nihon-u.ac.jp
N
N
Takenouchi-Ohkubo
M
M
Asano
H
H
Chihaya
W U
WU
Chung-Hsuing
K
K
Ishikasa
I
I
Moro
D015152:Blotting, Northern; D018938:Caco-2 Cells; D018076:DNA, Complementary / Q000235:genetics; D004357:Drug Synergism; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D019056:Receptors, Polymeric Immunoglobulin / Q000235:genetics / Q000378:metabolism*; D012635:Secretory Component / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects
D018076:DNA, Complementary; D016328:NF-kappa B; D012333:RNA, Messenger; D019056:Receptors, Polymeric Immunoglobulin; D012635:Secretory Component; D014409:Tumor Necrosis Factor-alpha; D014212:Tretinoin