Promotion of the growth of Crocus sativus cells and the production of crocin by rare earth elements.
La3+ and Ce3+, either singly or a mixture, promoted crocin production of Crocus sativus callus but Nd3+ had little effect and all metal ions were toxic above 100 microM. La3+ (60 microM) promoted growth of callus significantly but increased crocin only slightly. Ce3+ (40 microM) significantly promoted crocin production but had little effect on cell growth. La3+ (60 microM) and Ce3+ (20 microM) together gave the highest dry weight biomass (20.4 g l(-1)), crocin content (4.4 mg g(-1)) and crocin production (90 mg l(-1)) which were, respectively, 1.7-fold, 4.2-fold and 7.1-fold of those without additions. Nd3+ (40 microM) only slightly promoted cell growth and crocin production.
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100080, PR China. chenshu_an@yahoo.com
Shu-an
SA
Chen
Bing
B
Zhao
Xiaodong
X
Wang
Xiaofan
X
Yuan
Yuchun
Y
Wang
D002338:Carotenoids / Q000096:biosynthesis*; D002455:Cell Division / Q000187:drug effects; D027622:Crocus / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D008674:Metals, Rare Earth / Q000494:pharmacology*
D008674:Metals, Rare Earth; D002338:Carotenoids; C029036:crocin