[Effect of selection techniques on chromosome and ultrastructure of human spermatozoa].
OBJECTIVE
To know the effect of different selection techniques on chromosome abnormality rate and the percentage of spermatozoa with relatively intact head ultrastructure in human sperm.


METHODS
The selection techniques of swim-up, Percoll density gradient centrifugation and doule-flection tube were used to observe sperm chromosome abnormality rate, sex chromosomal ratio and the percentage of spermatozoa with relatively intact head ultrastructure.


RESULTS
The sperm chromosome abnormality rate, sex chromosomal ratio and the percentage of spermatozoa with relatively intact head ultrastructure remained unchanged after the application of the three selection techniques(P > 0.05).


CONCLUSIONS
The three selection techniques used in this study neither increase the damage to sperm ultrastructure nor exert effects on human chromosome.
Zhang
Qiaoyu
Q
Department of Obstetrics and Gynecology, Southwest Hospital, Third Military University, Chongqing 400038, China.
Qin
Rong
R
Chang
Qing
Q
Liang
Zhiqing
Z
Shi
Changxu
C
D000328:Adult; D002869:Chromosome Aberrations; D006801:Humans; D008297:Male; D013094:Spermatozoa / Q000648:ultrastructure*