Paracentrotus lividus eggs contain different RNAs at the animal and vegetal poles.
Paracentrotus lividus eggs were divided by centrifugation into nucleated and anucleated halves. Fertilization and development of the two halves permitted us to establish that nucleated and anucleated fragments correspond, respectively, to the animal and vegetal parts. RNA was extracted from both egg halves and submitted to differential display. Northern blot analysis confirmed their maternal origin and showed that each transcript has a different expression pattern during development. By Northern blot and in situ hybridization experiments we ascertained that Bep2 and PlAn1 are localized in the animal part, whereas 16S rRNA, Plveg1, and L27 in the vegetal part, and that Plun1 is uniformly distributed. Moreover, by treating P. lividus eggs with detergent, in presence or not of drugs such as colchicine and cytochalasin B, we demonstrated the involvement of the cytoskeleton only in localization of Bep2, PlAn1, and Plun1, suggesting that different mechanisms are utilized for animal and vegetal distribution.
Istituto di Biologia ed Immunologia Molecolare Alberto Monroy CNR, via Ugo La Malfa 153, 90146 Palermo, Italy. di-carlo@ibim.cnr.it
Marta
M
Di Carlo
Giovanna
G
Montana
Daniele P
DP
Romancino
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D036703:Blastula / Q000378:metabolism; D015152:Blotting, Northern; D002458:Cell Fractionation; D016764:Cell Polarity / Q000502:physiology; D002498:Centrifugation; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D017931:DNA Primers / Q000235:genetics; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010063:Ovum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012617:Sea Urchins / Q000196:embryology / Q000235:genetics / Q000378:metabolism*; D013501:Surface-Active Agents / Q000494:pharmacology
D017931:DNA Primers; D008565:Membrane Proteins; D012333:RNA, Messenger; D013501:Surface-Active Agents; D003571:Cytochalasin B; D012313:RNA; D003078:Colchicine