A simple and efficient method for isolating trichomes for downstream analyses.
Arabidopsis trichomes are an excellent cell type to address many questions in plant biology including the control of cell shape, endoreplication, and cell expansion. Because trichomes comprise such a small percentage of the cells of a leaf, biochemical analyses of trichomes are limited. To overcome this limitation, we developed a method for removing trichomes from the leaf surface. Our method allows the isolation of intact trichomes for use in downstream applications such as cell wall analysis, immunolocalization of trichome proteins, analysis of DNA content, and proteomics. Also, this method will facilitate the isolation of trichomes from practically any plant species.
Department of Botany and the University of Florida Genetics Institute, University of Florida, PO Box 118526, Gainesville, FL 32611-8526, USA.
Xiaoguo
X
Zhang
David G
DG
Oppenheimer
D017360:Arabidopsis / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism; D002498:Centrifugation; D002614:Chelating Agents / Q000494:pharmacology; D003584:Cytological Techniques / Q000295:instrumentation / Q000379:methods*; D018744:DNA, Plant / Q000032:analysis; D004533:Egtazic Acid / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D017830:Octoxynol / Q000494:pharmacology; D018515:Plant Leaves / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D010940:Plant Proteins / Q000032:analysis
D002614:Chelating Agents; D018744:DNA, Plant; D010940:Plant Proteins; D004533:Egtazic Acid; D017830:Octoxynol; D002118:Calcium