Phytochrome-mediated transcriptional up-regulation of ALLENE OXIDE SYNTHASE in rice seedlings.
Allene oxide synthase (AOS) is a key enzyme for the biosynthesis of jasmonic acid (JA). We identified four AOS gene homologs, named OsAOS1-4, in the database of a japonica rice genome and cloned a full-length cDNA of OsAOS1. The analysis of deduced amino acid sequences indicated that only OsAOS1 has a chloroplast transit peptide among all the identified monocot AOSs including OsAOSs. We found that the transcripts of OsAOS1 and OsAOS4 are up-regulated by red and far-red light in seedling shoots. The response in OsAOS1 transcripts occurred rapidly and transiently, while the response in OsAOS4 transcripts was slower and more sustainable; the maximal enhancement was greater in OsAOS1 transcripts than in OsAOS4 transcripts. The transcript of OsAOS1 was also up-regulated transiently in response to wounding, as reported for dicot AOSs. No wound-induced enhancement occurred, however, in OsAOS4 transcripts. Our results also indicated that OsAOS1, responding to both light and wounding, is the most highly expressed of all the OsAOSs in seedling shoots. By using phyA mutants of rice, it was demonstrated that the photoregulation of the AOS transcript level is mediated by phytochrome. It is suggested that this transcriptional photoregulation participates in the phytochrome-mediated inhibition of rice coleoptile growth.
Haga
Ken
K
Botanical Gardens, Research School of Science, Osaka City University, Kisaichi, Katano-shi, Osaka, 576-0004 Japan.
Iino
Moritoshi
M
D018506:Gene Expression Regulation, Plant / Q000502:physiology; D005809:Genes, Regulator / Q000235:genetics / Q000528:radiation effects; D018745:Genome, Plant; D019746:Intramolecular Oxidoreductases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D055537:Light Signal Transduction / Q000235:genetics; D012275:Oryza / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D010775:Photic Stimulation; D010834:Phytochrome / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000302:isolation & purification; D012038:Regeneration / Q000235:genetics / Q000528:radiation effects; D036226:Seedlings / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D015854:Up-Regulation / Q000235:genetics / Q000528:radiation effects; D014945:Wound Healing / Q000235:genetics / Q000528:radiation effects
D020033:Protein Isoforms; D010834:Phytochrome; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase