Determination of colchicine in Colchicum steveni and C. hierosolymitanum (Colchicaceae): comparison between two analytical methods.
The amounts of colchicine present in two Jordanian species of Colchicum, namely, C. steveni Kunth and C. hierosolymitanum Feibrun (Colchicaceae), have been determined. An HPLC-UV (photodiode array) method employing gradient elution was developed and the results compared with those obtained using a simple TLC-spectrophotometric method. The levels of colchicine as measured by these methods were not significantly different (p < 0.05) indicating that the spectrophotometric method is an acceptable alternative to HPLC. With respect to C. steveni, the leaves contained the largest amount of colchicine (0.204/100 g), whilst in C. hierosolymitanum corms showed the highest colchicine content (0.126/100 g). As a source of colchicine, the two investigated species showed levels comparable with those found in C. autumnale, the traditional source of colchicine.
Alali
Feras
F
Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan. falali@just.edu.jo
Tawaha
Khaled
K
Qasaymeh
Rana M
RM
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer; D003078:Colchicine / Q000032:analysis / Q000302:isolation & purification*; D003079:Colchicum / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D010936:Plant Extracts; D003078:Colchicine