Controlled microfluidic reconstitution of functional protein from an anhydrous storage depot.
A novel method has been developed for preserving molecules in microfluidic devices that also enables the control of the spatial and temporal concentrations of the reconstituted molecules within the devices. In this method, a storage cavity, embedded in a microchannel, is filled with a carbohydrate matrix containing, for example, a reagent. When the matrix is exposed to flowing liquid, it dissolves, resulting in the controlled reconstitution and release of the reagent from the cavity. The technique was demonstrated using two different model systems; the successful preservation and controlled release of beta-galactosidase was achieved. This method has possible applications for simple point-of-care drug delivery and immunoassays, and could be used to pattern the surfaces of microchannels. More broadly, this preservation and controlled release technique can be applied where the preservation and/or spatial and temporal control of chemical concentrations are desired.
Department of Bioengineering, University of Washington, Box 352141, Seattle, WA 98195-2255, USA.
Elena
E
Garcia
Jared R
JR
Kirkham
Anson V
AV
Hatch
Kenneth R
KR
Hawkins
Paul
P
Yager
D004129:Dimethylpolysiloxanes; D005453:Fluorescence; D044085:Microfluidics / Q000379:methods*; D009757:Nylons; D011309:Preservation, Biological / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D012996:Solutions; D013997:Time Factors; D001616:beta-Galactosidase / Q000737:chemistry
D004129:Dimethylpolysiloxanes; D009757:Nylons; D011506:Proteins; D012996:Solutions; C085707:poly(dimethylsiloxane)-polyamide copolymer; D001616:beta-Galactosidase