Role of Syndecan-4 in the cellular invasion of Orientia tsutsugamushi.
Cell surface heparan sulfate proteoglycans (HSPGs) play a critical role in the cellular invasion of intracellular bacteria and are presumed to have a role in the infection of host cells by Orientia tsutsugamushi. Previously, we showed that O. tsutsugamushi infection decreased markedly after treating host cells with heparinase, which suggests that HSPGs play an important role in oriential infection. We tested oriential infection in REF-Syn4 cells over-expressing syndecan-4, and in REF-Syn4AS cells in which the expression of syndecan-4 was down regulated by transfecting with anti-sense syndecan-4 cDNA. Oriential infection was found to be dependent on the expression level of syndecan-4 on the cell surface. Furthermore, the infectivity of O. tsutsugamushi was specifically reduced by treating O. tsutsugamushi with the purified recombinant core protein of syndecan-4 (Syn4E). These results suggest that the core protein of syndecan-4 and the heparin/heparan sulfate chain of syndecan play an important role in oriential infection by O. tsutsugamushi.
Kim
Hang-Rae
HR
Department of Microbiology and Immunology, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, Seoul 110-799, South Korea.
Choi
Myung-Sik
MS
Kim
Ik-Sang
IS
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry / Q000502:physiology; D006224:Cricetinae; D016373:DNA, Antisense; D005347:Fibroblasts / Q000737:chemistry* / Q000378:metabolism / Q000382:microbiology*; D005786:Gene Expression Regulation; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism; D006493:Heparin / Q000378:metabolism; D019763:Heparin Lyase / Q000378:metabolism; D006801:Humans; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D051379:Mice; D012285:Orientia tsutsugamushi / Q000254:growth & development* / Q000378:metabolism / Q000472:pathogenicity*; D011509:Proteoglycans / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D051381:Rats; D053671:Syndecan-4
D016373:DNA, Antisense; D019812:Heparan Sulfate Proteoglycans; D008562:Membrane Glycoproteins; D011509:Proteoglycans; C508304:SDC4 protein, human; C508306:Sdc4 protein, mouse; C508307:Sdc4 protein, rat; D053671:Syndecan-4; D006493:Heparin; D019763:Heparin Lyase