Molecularly imprinted TiO2-matrix-assisted laser desorption/ionization mass spectrometry for selectively detecting alpha-cyclodextrin.
This study describes a new means to conduct molecular recognition-based analysis using mass spectrometry. Taking advantage of the unique characteristic of the absorption capacity of the TiO(2) sol-gel material in the UV region, a TiO(2) sol-gel-deposited thin film was employed as the sample substrate to assist in UV laser desorption/ionization of analytes. Sol-gels are polymeric materials that are easy to prepare and modify at low temperatures. Molecularly imprinted TiO(2) sol-gels were generated for molecular recognition-based analysis. alpha-Cyclodextrin (CD) was selected as the template molecule and doped into TiO(2) in a sol-gel reaction. The molecularly imprinted TiO(2) sol was spin-coated on a glass slide, and appropriate template cavities in the TiO(2) sol-gel material were formed after the template molecules were removed. We demonstrate that this modified glass slide can be used to select alpha-CD from a sample solution containing equal amounts of alpha-, beta-, and gamma-CD (50 ppb each, 18 mL); alpha-CD was directly detected from the modified glass slide by matrix-assisted laser desorption/ionization mass spectrometry without the addition of extra matrix. This approach provides a new detection method for molecular recognition-based analysis.
Chen
Cheng-Tai
CT
Department of Applied Chemistry, National Chiao Tung University, Hsinchu 300, Taiwan.
Chen
Yu-Chie
YC
D001920:Bradykinin / Q000032:analysis; D003505:Cyclodextrins / Q000032:analysis*; D045304:Cytochromes c / Q000032:analysis; D007328:Insulin / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013053:Spectrophotometry; D014025:Titanium / Q000737:chemistry*; D047391:alpha-Cyclodextrins
D003505:Cyclodextrins; D007328:Insulin; D047391:alpha-Cyclodextrins; C009495:titanium dioxide; D045304:Cytochromes c; D014025:Titanium; D001920:Bradykinin; C032613:alpha-cyclodextrin