ACYP1 gene possesses two alternative splicing forms that induce apoptosis.
In human cell lines two products of the ACYP1 gene were detected by RT-PCR. In addition to the expected amplicon corresponding to the CT form of acylphosphatase (320 bp) a second unexpected one (400 bp) was characterized as the result of an alternative splicing in which an extra 79 bp long exon is inserted between the two known exons. This new product, indicated as CTsv, was cloned and expressed. We performed the ectopic expression of the two alternative splicing forms. Both CT and CTsv products were able to induce a proapoptotic effect when expressed in HeLa cell line, despite the fact that the CTsv protein did not show any acylphosphatase activity.
Dipartimento di Scienze Biochimiche, Università degli Studi di Firenze, Viale Morgagni 50, Firenze 50134, Italy. donatella.deglinnocenti@unifi.it
Donatella
D
Degl'Innocenti
Riccardo
R
Marzocchini
Francesca
F
Malentacchi
Matteo
M
Ramazzotti
Giovanni
G
Raugei
Giampietro
G
Ramponi
D017766:Acid Anhydride Hydrolases / Q000235:genetics* / Q000502:physiology; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D005091:Exons; D006367:HeLa Cells; D006801:Humans; D007438:Introns; D007527:Isoenzymes / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D000097024:Acylphosphatase
D017766:Acid Anhydride Hydrolases; D004247:DNA; D007527:Isoenzymes; D011993:Recombinant Fusion Proteins; D000097024:Acylphosphatase