DNM1DN: a new class of paralogous genomic segments (duplicons) with highly conserved copies on chromosomes Y and 15.
Screening a testis cDNA selection library for Y-linked genes yielded 79 cDNAs. Of these, 9 matched the 3' region of the dynamin 1 gene (DNM1) on chromosome 9q34 with >90% identity. Fluoresence in situ hybridisation and PCR amplification were used to localise a large number of DNM1-like sequences to human chromosomes 15 and Y. PCR amplification of overlapping Y-linked YACs allowed a more accurate mapping of the Y-linked DNM1-like cDNAs to a euchromatic locus in close proximity to heterochromatin at Yq11.23. A search of the genome database identified 64 highly homologous copies of the DNM1 fragment. Most of these copies were localised to chromosomes 15 and Y, but others mapped to chromosomes 5, 8, 10, 12, 19 and 22. These sequences exhibit all the major features of a duplicon and have been designated DNM1DN (DNM1 duplicon). Evolutionary studies using fluorescence in situ hybridisation indicate that transposition of the DNM1DN sequence to chromosome 15 took place earlier in primate evolution than the transposition to the Y chromosome. The translocation to the Y took place at a time following the divergence of a common ancestor from gorilla, approximately 4-7 million years ago.
MRC Human Biochemical Genetics Unit, University College London, Wolfson House, London NW1 2HE, UK. e.makrinou@prion.ucl.ac.uk
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Makrinou
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Fox
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Wolfe
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Cameron
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Taylor
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YH
Edwards
D000818:Animals; D002874:Chromosome Mapping; D002884:Chromosomes, Human, Pair 15; D041322:Chromosomes, Human, Y; D033481:Chromosomes, Mammalian; D017124:Conserved Sequence; D018076:DNA, Complementary; D034284:Dynamin I / Q000235:genetics*; D019143:Evolution, Molecular; D015723:Gene Library; D020131:Genes, Duplicate; D015894:Genome, Human; D006071:Gorilla gorilla / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D005810:Multigene Family; D002679:Pan troglodytes / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013737:Testis; D014998:Y Chromosome
D018076:DNA, Complementary; D034284:Dynamin I