A new method for purification of carbonic anhydrase isozymes by affinity chromatography.
A new affinity gel for purification of carbonic anhydrase isozymes was prepared using EUPERGIT C-250L derivatized with p-aminobenzenesulfonamide, an inhibitor of carbonic anhydrase. The binding capacity of the affinity gel was determined at different temperatures, pH values, elution buffers, and ionic strengths. Human carbonic anhydrase isozymes (HCA I and HCA II) and bovine carbonic anhydrase (BCA) were purified in high yields from erythrocytes.
Balikesir University, Science and Art Faculty, Department of Chemistry/Biochemistry Section, 10100 Balikesir, Turkey.
O
O
Ozensoy
O
O
Arslan
S Oznur
SO
Sinan
D000818:Animals; D002256:Carbonic Anhydrases / Q000302:isolation & purification*; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000302:isolation & purification; D009994:Osmolar Concentration; D012117:Resins, Synthetic; D013449:Sulfonamides / Q000737:chemistry*
D007527:Isoenzymes; D012117:Resins, Synthetic; D013449:Sulfonamides; D002256:Carbonic Anhydrases