Opg, Rank, and Rankl in tooth development: co-ordination of odontogenesis and osteogenesis.
Osteoprotegerin (OPG), receptor activator of nuclear factor-kappaB (RANK), and RANK ligand (RANKL) are mediators of various cellular interactions, including bone metabolism. We analyzed expression of these three genes during murine odontogenesis from epithelial thickening to cytodifferentiation stages. Opg showed expression in the thickening and bud epithelium. Expression of Opg and Rank was observed in both the internal and the external enamel epithelium as well as in the dental papilla mesenchyme. Although Rankl expression was not detected in tooth epithelium or mesenchyme, it was expressed in pre-osteogenic mesenchymal cells close to developing tooth germs. All three genes were detected in developing dentary bone at P0. The addition of exogenous OPG to explant cultures of tooth primordia produced a delay in tooth development that resulted in reduced mineralization. We propose that the spatiotemporal expression of these molecules in early tooth and bone primordia cells has a role in co-ordinating bone and tooth development.
Department of Craniofacial Development, Floor 28, Guy's Tower, GKT Dental Institute, King's College London, Guy's Hospital, London Bridge, London SE1 9RT, UK.
A
A
Ohazama
J-M
JM
Courtney
P T
PT
Sharpe
D000539:Alveolar Process / Q000166:cytology; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D046508:Culture Techniques; D003743:Dental Enamel / Q000166:cytology; D003771:Dental Papilla / Q000166:cytology; D004847:Epithelial Cells / Q000166:cytology; D006023:Glycoproteins / Q000235:genetics / Q000502:physiology*; D008024:Ligands; D008562:Membrane Glycoproteins / Q000235:genetics / Q000502:physiology*; D008648:Mesoderm / Q000166:cytology; D051379:Mice; D008815:Mice, Inbred Strains; D016328:NF-kappa B / Q000235:genetics / Q000502:physiology*; D009805:Odontogenesis / Q000235:genetics / Q000502:physiology*; D010012:Osteogenesis / Q000235:genetics / Q000502:physiology*; D053244:Osteoprotegerin; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000502:physiology*; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics / Q000502:physiology*; D014074:Tooth Calcification / Q000235:genetics / Q000502:physiology; D014083:Tooth Germ / Q000166:cytology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000502:physiology*
D002352:Carrier Proteins; D006023:Glycoproteins; D008024:Ligands; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D053244:Osteoprotegerin; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D018160:Receptors, Cytoplasmic and Nuclear; D018124:Receptors, Tumor Necrosis Factor; C505687:Tnfrsf11a protein, mouse; C506031:Tnfrsf11b protein, mouse; C505689:Tnfsf11 protein, mouse; D014409:Tumor Necrosis Factor-alpha