Detection of streptomycetes in house dust--comparison of culture and PCR methods.
Streptomycetes are gram-positive, spore producing, filamentous bacteria common in soil, but also present in indoor environments. They are potent producers of secondary metabolites and inducers of inflammatory responses in vitro. Polymerase chain reaction (PCR)- and culture-based detection methods for streptomycetes in house dust samples were compared. A total of 47 dust samples were investigated, and the presence of streptomycetes was determined by cultivation on tryptone-yeast-extract-glucose agar and PCR. The 16S rRNA gene of actinomycete isolates from house dust was partially sequenced to investigate if they belong to the genus Streptomyces. Both PCR and culture showed more frequent occurrence of streptomycetes in moisture-damaged homes, although the results did not correlate well. The occurrence of streptomycetes in house dust was associated with moisture damage of the home. The amount of Streptomyces-specific PCR amplification product was significantly higher in dust from moisture-damaged homes than in homes with no moisture damage (P < 0.05, Mann-Whitney U test). A correlation between streptomycetes and moisture damage, although not statistically significant, was also observed when using binary data, e.g. presence or absence of streptomycetes or moisture damage (P = 0.054 for PCR, and P = 0.127 for culture, Fisher's exact test). Altogether, the presence of streptomycetes in house dust seems to indicate the presence of moisture damage in the building.
Laboratory of Environmental Microbiology, National Public Health Institute (KTL), PO Box 95, FIN-70701 Kuopio, Finland. helena.rintala@ktl.fi
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Rintala
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Hyvärinen
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Paulin
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Nevalainen
D016902:Air Pollution, Indoor / Q000032:analysis*; D001431:Bacteriological Techniques; D004269:DNA, Bacterial; D006798:Housing; D016133:Polymerase Chain Reaction; D013171:Spores, Bacterial; D013302:Streptomyces / Q000235:genetics / Q000302:isolation & purification*; D014867:Water
D004269:DNA, Bacterial; D014867:Water