Identification of genes responsive to apoptosis in HL-60 cells.
OBJECTIVE
To identify genes responsive to apoptosis in HL-60 cells treated by homoharringtonine.


METHODS
cDNA microarray technology was used to detect gene expression and the result of microarrays for genes (TIEG and VDUP1) was confirmed by Northern analysis.


RESULTS
Seventy-five individual mRNAs whose mass changed significantly were identified. Among these genes (25 were up-regulated and 50 were down-regulated), most are known related to oncogenes and tumor suppressor. Some genes were involved in apoptosis signaling pathways.


CONCLUSIONS
TGF beta and TNF apoptosis signaling pathways were initiated during apoptosis in HL-60 cells. TIEG and VDUP1 play important roles in mediating apoptosis.
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Wei
W
Jin
Le-feng
LF
Qu
Ping
P
Min
Shan
S
Chen
Hong
H
Li
He
H
Lu
Yong-tai
YT
Hou
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000235:genetics*; D020869:Gene Expression Profiling; D018922:HL-60 Cells; D006248:Harringtonines / Q000494:pharmacology*; D000077863:Homoharringtonine; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis
D000972:Antineoplastic Agents, Phytogenic; D006248:Harringtonines; D000077863:Homoharringtonine