Effect of ultrasound on structure and functional properties of antithrombin III and proteins of PPSB complex.
A combination of gel-permeation HPLC, affinity chromatography on heparin-Sepharose, gel electrophoresis, and estimation of inhibitory activity showed that effect of low-frequency ultrasound (26 W/cm(2), 37 degrees C, pH 7.4) on homogeneous antithrombin III was accompanied by formation of aggregates and a latent form of serpin. Heparin and pentosan polysulfate stabilized antithrombin III; this resulted in decrease in ultrasonic-induced formation of the aggregate and latent forms. The influence of ultrasound was not accompanied by significant changes in the contents of non-activated blood coagulation factors in the PPSB complex.
Research Institute of Physical Chemical Problems, Belarusian State University, Minsk 220050, Belarus. biopharm@bsu.by
V M
VM
Shkumatov
I E
IE
Adzerikho
J A
JA
Lesnikovich
E A
EA
Cherniavsky
D000925:Anticoagulants / Q000737:chemistry*; D000990:Antithrombin III / Q000737:chemistry* / Q000302:isolation & purification; D001777:Blood Coagulation; D001779:Blood Coagulation Factors / Q000737:chemistry* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D020552:Comet Assay; D004789:Enzyme Activation; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D010426:Pentosan Sulfuric Polyester / Q000737:chemistry*; D014465:Ultrasonics
D000925:Anticoagulants; D001779:Blood Coagulation Factors; C025667:prothrombin complex concentrates; D010426:Pentosan Sulfuric Polyester; D000990:Antithrombin III; D006493:Heparin