The use of novel water-in-oil microemulsions in microemulsion electrokinetic chromatography.
We describe the novel use of water-in-oil (W/O) microemulsions to achieve unique separations in microemulsion electrokinetic chromatography (MEEKC). The choice and concentration of the buffer type, surfactant and co-surfactant were all examined and optimized. Separations of a range of neutral and acidic analytes was shown to be markedly different to that obtained by (oil-in-water) O/W MEEKC. Neutral solutes are separated by virtue of their solubility (log P) values in O/W MEEKC with the more water-insoluble solutes migrating last. This separation process does not occur in W/O, as neutral solutes are not separated in order of log P.
Pharmaceutical Development GlaxoSmithKline R&D, Harlow, Essex, UK.
Kevin D
KD
Altria
Margo F
MF
Broderick
Sheila
S
Donegan
Joe
J
Power
D000098:Acetophenones / Q000032:analysis; D002021:Buffers; D002110:Caffeine / Q000032:analysis; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004655:Emulsions; D006863:Hydrogen-Ion Concentration; D009281:Naphthalenes / Q000032:analysis; D009821:Oils / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000032:analysis*; D012995:Solubility; D013011:Sorbic Acid / Q000032:analysis; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature; D013890:Thiourea / Q000032:analysis; D014867:Water
D000098:Acetophenones; D002021:Buffers; D004655:Emulsions; D009281:Naphthalenes; D009821:Oils; D004364:Pharmaceutical Preparations; D013501:Surface-Active Agents; D014867:Water; C031721:naphthalene; D002110:Caffeine; C031335:4-hydroxyacetophenone; D013890:Thiourea; D013011:Sorbic Acid