Reagentless mechanical cell lysis by nanoscale barbs in microchannels for sample preparation.
A highly effective, reagentless, mechanical cell lysis device integrated in microfluidic channels is reported. Sample preparation, specifically cell lysis, is a critical element in 'lab-on-chip' applications. However, traditional methods of cell lysis require purification steps or complicated fabrication steps that a simple mechanical method of lysis may avoid. A simple and effective mechanical cell lysis system is designed, microfabricated, and characterized to quantify the efficiency of cell lysis and biomolecule accessibility. The device functionality is based on a microfluidic filter region with nanostructured barbs created using a modified deep reactive ion etching process. Mechanical lysis is characterized by using a membrane impermeable dye. Three main mechanisms of micro-mechanical lysis are described. Quantitative measurements of accessible protein as compared to a chemically lysed sample are acquired with optical absorption measurements at 280 and 414 nm. At a flow rate of 300 microL min(-1) within the filter region total protein and hemoglobin accessibilities of 4.8% and 7.5% are observed respectively as compared to 1.9% and 3.2% for a filter without nanostructured barbs.
Di Carlo
Dino
D
Berkeley Sensor and Actuator Center, Department of Bioengineering, University of California, Berkeley, CA 94720, USA.
Jeong
Ki-Hun
KH
Lee
Luke P
LP
D000818:Animals; D002477:Cells / Q000737:chemistry*; D004912:Erythrocytes / Q000737:chemistry; D018922:HL-60 Cells; D006801:Humans; D066298:In Vitro Techniques; D036103:Nanotechnology; D011506:Proteins / Q000737:chemistry; D012756:Sheep
D011506:Proteins