RGA protein associates with a TRPV ion channel during biosynthesis and trafficking.
TRPV ion channels transduce a range of temperature stimuli. We proposed that analysis of the protein-protein interactions made by TRPV2 might give insight into the key issues surrounding this channel. These issues include the potential functional significance of TRPV2 in non-sensory tissues, the molecules involved in transducing its activation signal(s) and the mechanism by which its trafficking to the cell surface is regulated. Here we describe the interaction of TRPV2 channel with the RGA gene product. RGA is a four-transmembrane domain, intracellularly localized protein. RGA associates with TRPV2 in a rat mast cell line that is a native context for both proteins. The interaction between TRPV2 and RGA is transient and occurs intracellularly. RGA does not accompany TRPV2 to the cell surface. Formation of the TRPV2/RGA complex is dependent upon a cellular glycosylation event, suggesting that RGA may play a chaperone or targeting role for TRPV2 during the maturation of the ion channel protein. These data record a novel protein-protein interaction for TRPV2 and provide a foundation for future study of the potential regulatory contribution of RGA to TRPV2 function.
Laboratory of Cell Biology and Immunology, Queen's Center for Biomedical Research at The Queen's Medical Center, Honolulu, Hawaii 96813, USA.
Jason C
JC
Barnhill
Alexander J
AJ
Stokes
Murielle
M
Koblan-Huberson
Lori M N
LM
Shimoda
Atsushi
A
Muraguchi
Chaker N
CN
Adra
Helen
H
Turner
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010802:Phylogeny; D011485:Protein Binding / Q000187:drug effects; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011955:Receptors, Drug / Q000235:genetics / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D050916:TRPV Cation Channels; D014415:Tunicamycin / Q000494:pharmacology; D020798:Two-Hybrid System Techniques
D007473:Ion Channels; D008565:Membrane Proteins; D012333:RNA, Messenger; D011955:Receptors, Drug; C493780:Slc50a1 protein, mouse; D050916:TRPV Cation Channels; C476722:Trpv2 protein, rat; D014415:Tunicamycin