Polo-like kinase 3 is Golgi localized and involved in regulating Golgi fragmentation during the cell cycle.
The Golgi apparatus undergoes extensive fragmentation during mitosis in animal cells. Protein kinases play a critical role during fragmentation of the Golgi apparatus. We reported here that Polo-like kinase 3 (Plk3) may be an important mediator during Golgi breakdown. Specifically, Plk3 was concentrated at the Golgi apparatus in HeLa and A549 cells during interphase. At the onset of mitosis, Plk3 signals disintegrated and redistributed in a manner similar to those of Golgi stacks. Nocodazole activated Plk3 kinase activity, correlating with redistribution of Plk3 signals and Golgi fragmentation. In addition, treatment with brefeldin A (BFA), a Golgi-specific poison, also resulted in disappearance of concentrated Plk3 signals. Plk3 interacted with giantin, a Golgi-specific protein. Expression of Plk3, but not the kinase-defective Plk3 (Plk3(K52R)), resulted in significant Golgi breakdown. Given its role in cell cycle progression, Plk3 may be a protein kinase involved in regulation of Golgi fragmentation during the cell cycle.
Ruan
Qin
Q
Department of Medicine, New York Medical College, Valhalla, NY 10595, USA.
Wang
Qi
Q
Xie
Suqing
S
Fang
Yuqiang
Y
Darzynkiewicz
Zbigniew
Z
Guan
Kunliang
K
Jhanwar-Uniyal
Meena
M
Dai
Wei
W
D020126:Brefeldin A / Q000494:pharmacology; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000032:analysis* / Q000502:physiology*; D002460:Cell Line; D006056:Golgi Apparatus / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D000077614:Golgi Matrix Proteins; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D015739:Nocodazole / Q000494:pharmacology; D017346:Protein Serine-Threonine Kinases / Q000032:analysis* / Q000502:physiology*; D025521:Tumor Suppressor Proteins; D000097868:Polo-like Kinases
D020126:Brefeldin A; D018797:Cell Cycle Proteins; D000077614:Golgi Matrix Proteins; D008565:Membrane Proteins; D015739:Nocodazole; D017346:Protein Serine-Threonine Kinases; D025521:Tumor Suppressor Proteins; C087574:macrogolgin; C486256:PLK3 protein, human; D000097868:Polo-like Kinases