[Spectra study on the varied features of crocidolite fibers in rat].
OBJECTIVE
To further study the pathogenic mechanism of crocidolite, the imperceptible changes of crocidolite surface in rat were observed.


METHODS
The animal model was established and the changes in the rat infected with dust were observed by use of microscopy, SEM, differential thermal analysis and IR spectroscopy.


RESULTS
In the course of interaction between organism protein and crocidolite, the protein symmetry decreased and structure loosened. The silicon of crocidolite was bonded with the alkyl, amido- of protein. New absorption bands of Si-O-C(N), Si-R clearly appeared. The organism cleared the dust by means of dissolution, enwrapping, winding or in the way of biochemical dissolution, and the fibre became shortened, broken, bifurcated, ends-rounded, and also it could dissolve, transfer and chemically react on surface.


CONCLUSIONS
The results showed that the surface radicals of asbestos fibre reacted with some albumen in tissue and hence formed new surface mediator. It is a new form of dissolution and reaction of fibre in vivo that fibres in alveoli transform to carbonate. The residual substances of crocidolite are mainly Si-O. Tissue membrane is the retardation cingulum of dust transference in vivo.
Dong
Fa-qin
FQ
Institue of Mineral Materials and Application, Southwest University of Science & Technology, Mianyang 621002, China.
Luo
Su-qiong
SQ
D000818:Animals; D017638:Asbestos, Crocidolite / Q000633:toxicity*; D001195:Asbestosis / Q000473:pathology; D004391:Dust; D008855:Microscopy, Electron, Scanning; D019016:Mineral Fibers / Q000633:toxicity; D010995:Pleural Diseases / Q000209:etiology / Q000473:pathology*; D011658:Pulmonary Fibrosis / Q000209:etiology / Q000473:pathology*; D051381:Rats; D012825:Silicon
D004391:Dust; D019016:Mineral Fibers; D017638:Asbestos, Crocidolite; D012825:Silicon