Detection of Helicobacter pylori by PCR on gastric biopsy specimens taken for CP test: comparison with histopathological analysis.
The aims of the present study were: (i) to assess whether H. pylori could be successfully detected by PCR from the same biopsy sample used for CPtest; and ii) to evaluate CPtest comparatively to both PCR and histology for detection of H. pylori infection in dyspeptic patients. Three antral gastric biopsies were collected from each of 80 consecutive dyspeptic patients undergoing oesophago-gastroduodenoscopy. Two biopsies were for histology (gold standard), one for CPtest, scored at 20min, 1h and 24h for the presence of urease activity. Gastric biopsy was then removed from CPtest and used for ureC-targeted PCR. Fifty-five (68.7%) patients were positive for H. pylori infection by histology. CPtest yielded an overall diagnostic accuracy of 93.8% (95% CI: 91-96.4%), regardless of observation period. No erroneous categorization of H. pylori status occurred using PCR, yielding sensitivity, specificity, positive and negative predictive values, and overall diagnostic accuracy of 100%. Our results suggest that H. pylori can be detected by PCR in gastric biopsies previously taken for CPtest, so reducing the workload of the endoscopist by saving additional biopsies for culture analysis and susceptibility tests.
Department of Biomedical Sciences, Clinical Microbiology Laboratory, and Center for Excellence on Aging, G. d'Annunzio University, School of Medicine, Chieti, Italy.
G
G
Di Bonaventura
M
M
Neri
D
D
Angelucci
S
S
Rosini
M
M
Piccolomini
R
R
Piccolomini
D000328:Adult; D016481:Helicobacter Infections / Q000175:diagnosis / Q000201:enzymology; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology / Q000382:microbiology / Q000473:pathology; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D011706:Pyloric Antrum / Q000201:enzymology* / Q000382:microbiology* / Q000473:pathology; D014510:Urease / Q000032:analysis*
D014510:Urease