Identification and quantification of components in extracts of Uncaria tomentosa by HPLC-ES/MS.
The two main classes of secondary metabolites, alkaloids and quinovic acid glycosides, of Uncaria tomentosa (Willd.) DC. (Rubiaceae), a Peruvian plant commonly known as 'uña de gato', have been analysed. Separation of the alkaloidal fraction was achieved using a solid phase extraction method based on cationic exchange, and an analytical method employing HPLC-ES/MS has been developed. Quantitative data for commercial wild bark, cultivated bark and leaves are reported. The analysis of quinovic acid glycosides was performed directly on the crude extract using both a fast analytical method based on flow injection ES/MS, and a more complete analytical technique using HPLC-MS.
Dipartimento di Scienze Farmaceutiche, Facoltá di Farmacia, Universitá di Salerno, Via Ponte don Melillo, 84084 Fisciano, Italy. pmontoro@unisa.it
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D000470:Alkaloids / Q000302:isolation & purification*; D020948:Cat's Claw / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006027:Glycosides / Q000302:isolation & purification*; D010568:Peru; D024301:Plant Bark / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D014315:Triterpenes / Q000302:isolation & purification*
D000470:Alkaloids; D006027:Glycosides; D014315:Triterpenes; C472605:quinovic acid