Characterization of supercritical fluid extracts of St. John's wort (Hypericum perforatum L.) by HPLC-MS and GC-MS.
Supercritical fluid extracts (carbon dioxide without modifiers) of St. John's Wort (Hypericum perforatum L., Clusiaceae) were analyzed by GC-MS, HPLC-DAD and HPLC-DAD-MS. Besides the dominating phloroglucinols hyperforin (36.5 +/- 1.1%) and adhyperforin (4.6 +/- 0.1%), the extracts mainly contained alkanes (predominantly nonacosane), fatty acids and wax esters. The apolar components tended to accumulate in a waxy phase resting a top of the hyperforin-enriched phase. No components of higher polarity like naphthodianthrones were found. A set of hyperforin oxidation products was detected and tentatively assigned using HPLC-MS.
Seger
Christoph
C
Department of Pharmaceutical Technology, Institute of Pharmacy, Leopold-Franzens University, Innsbruck, Austria. chrostoph.seger@uibk.ac.at
Römpp
Harald
H
Sturm
Sonja
S
Haslinger
Ernst
E
Schmidt
Peter C
PC
Hadacek
Franz
F
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D020902:Hypericum; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry*
D010936:Plant Extracts