Mitotic phosphorylation of histone H3 at threonine 3.
Nuclear envelope-peripheral heterochromatin fractions contain multiple histone kinase activities. In vitro assays and amino-terminal sequencing show that one of these activities co-isolates with heterochromatin protein 1 (HP1) and phosphorylates histone H3 at threonine 3. Antibodies recognizing this post-translational modification reveal that in vivo phosphorylation at threonine 3 commences at early prophase in the vicinity of the nuclear envelope, spreads to pericentromeric chromatin during prometaphase and is fully reversed by late anaphase. This spatio-temporal pattern is distinct from H3 phosphorylation at serine 10, which also occurs during cell division, suggesting segregation of differentially phosphorylated chromatin to different regions of mitotic chromosomes.
Department of Basic Sciences, The University of Crete, School of Medicine, 95110 Heraklion, Crete, Greece.
Hara
H
Polioudaki
Yolanda
Y
Markaki
Niki
N
Kourmouli
George
G
Dialynas
Panayiotis A
PA
Theodoropoulos
Prim B
PB
Singh
Spyros D
SD
Georgatos
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000737:chemistry; D004910:Erythrocyte Membrane / Q000737:chemistry; D004912:Erythrocytes / Q000166:cytology; D005982:Glutathione Transferase / Q000378:metabolism; D006570:Heterochromatin / Q000737:chemistry; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D008938:Mitosis; D009685:Nuclear Envelope / Q000737:chemistry; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D013912:Threonine / Q000378:metabolism*; D014422:Turkeys / Q000097:blood
D006570:Heterochromatin; D006657:Histones; D011993:Recombinant Fusion Proteins; D013912:Threonine; D005982:Glutathione Transferase