Systemic and regional hemorheological consequences of warm and cold hind limb ischemia-reperfusion in a canine model.
We have studied systemic and regional changes in hemorheological parameters after complete acute limb ischemia and reperfusion (I/R) in 24 mongrel dogs. Unilateral cooled and non-cooled vascular ischemia (3 h)-reperfusion (4 h), and sham-operations were performed. Blood samples were collected from the excluded region, during reperfusion and for 5 days. Whole blood and plasma viscosity (WBV, PV), relative cell transit time (RCTT) of erythrocytes, fibrinogen level and hematological parameters were determined. In I/R groups WBV of excluded blood was significantly higher compared to the base (p < 0.05), and RCTT increased during the reperfusion. On 2nd-3rd days RCTT increased significantly in both I/R groups. In each group PV and fibrinogen showed continuous increase during the postoperative period, prominently in cooled I/R group, and furthermore WBV corrected for hematocrit (40%) was the highest in cooled I/R group. These suggest that surgical acute limb I/R may cause hemorheological changes, which are more serious after cooling. (Grants: OTKA-T032571, 6003/1/2001/ETT.)
Nemeth
N
N
Department of Operative Techniques and Surgical Research, Medical and Health Science Centre, Faculty of Medicine, University of Debrecen, Hungary. nemeth@jaguar.dot.hu
Szokoly
M
M
Acs
G
G
Brath
E
E
Lesznyak
T
T
Furka
I
I
Miko
I
I
D000818:Animals; D001809:Blood Viscosity; D001831:Body Temperature; D003080:Cold Temperature / Q000009:adverse effects; D004285:Dogs; D004907:Erythrocyte Deformability; D004909:Erythrocyte Indices; D005340:Fibrinogen / Q000032:analysis; D006400:Hematocrit; D018056:Hemorheology; D006614:Hindlimb / Q000098:blood supply*; D007511:Ischemia / Q000097:blood* / Q000503:physiopathology; D023421:Models, Animal; D015427:Reperfusion Injury / Q000097:blood*
D005340:Fibrinogen