Trimethyltin-induced differential expression of PAR subtypes in reactive astrocytes of the rat hippocampus.
Thrombin, its main inhibitor (protease nexin-1) and its related receptors (protease-activated receptors, PAR-1,-2, -3, -4) were studied in rat hippocampus following administration of trimethyltin (TMT), a neurotoxin inducing neuronal degeneration and reactive gliosis. Reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry revealed that while expression of prothrombin and protease nexin-1 did not change significantly in TMT-treated hippocampi, PARs (in particular PAR-1 and to a lesser extent PAR-2 and PAR-3) were upregulated in reactive astrocytes, suggesting their involvement in neurodegeneration and in the consequent response of the nervous tissue.
Pompili
Elena
E
Department of Cardiovascular Sciences, University "La Sapienza", Via A. Borelli 50, Rome 00161, Italy. elena.pompili@uniroma1.it
Nori
Stefania Lucia
SL
Geloso
Maria Concetta
MC
Guadagni
Enrico
E
Corvino
Valentina
V
Michetti
Fabrizio
F
Fumagalli
Lorenzo
L
D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects; D007150:Immunohistochemistry / Q000379:methods; D009498:Neurotoxins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D044462:Receptors, Proteinase-Activated / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014298:Trimethyltin Compounds / Q000494:pharmacology*; D015854:Up-Regulation
D009498:Neurotoxins; D012333:RNA, Messenger; D044462:Receptors, Proteinase-Activated; D014298:Trimethyltin Compounds; C046488:trimethyltin