Pseudophosphorylation of tau protein alters its ability for self-aggregation.
Filamentous tau protein deposits are a pathological hallmark of a group of neurodegenerative disorders (tauopathies). Tau protein in these aggregates is highly phosphorylated at different phosphorylation sites. Although tau filaments can be formed by heparin-induced aggregation of unphosphorylated recombinant tau, it is not known how tau phosphorylation modulates aggregation behaviour. Analysis of the effect of tau phosphorylation at defined single or multiple sites is hampered by the low specificity of protein kinases and the highly dynamic turnover of phosphorylation in vivo. To overcome this problem we employed site-directed mutagenesis to convert serine and threonine to aspartic acid or glutamic acid, which introduce a negative charge and conformational change that mimic phosphorylation. We tested 14 different mutated tau proteins for their propensity for self-aggregation and formation of tau filaments. Tau aggregation was monitored with thioflavin S fluorescence in the presence of different inducers such as heparin, Al3+, Fe2+ and Fe3+. We found that mutations in the N-terminal portion up to amino acid 208 mainly suppress tau aggregation, whereas mutations in the C-terminal region mainly lead to an enhanced aggregation. Mutations in the middle portion of tau showed a mixed picture of suppression and enhancement of aggregation. A single amino acid change Ser422Glu has aggregation-favouring properties with all four inducers.
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Paul Flechsig Institute of Brain Research, Department of Neuroanatomy, University of Leipzig, Leipzig, Germany.
Stieler
J T
JT
Arendt
T
T
Holzer
M
M
D019943:Amino Acid Substitution; D000818:Animals; D052160:Benzothiazoles; D006493:Heparin / Q000737:chemistry / Q000378:metabolism; D046911:Macromolecular Substances; D008670:Metals / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D008870:Microtubules / Q000737:chemistry / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D010766:Phosphorylation; D011485:Protein Binding / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D011487:Protein Conformation; D013844:Thiazoles / Q000737:chemistry; D016875:tau Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D052160:Benzothiazoles; D046911:Macromolecular Substances; D008670:Metals; D013844:Thiazoles; D016875:tau Proteins; C009462:thioflavin T; D006493:Heparin