Campylobacter spp. in Irish feedlot cattle: a longitudinal study involving pre-harvest and harvest phases of the food chain.
The aim of this study was to investigate faecal shedding and transmission of Campylobacter spp. in cohorts of cattle within a feedlot, to assess subsequent contamination of carcasses with this pathogen and to identify risk factors associated with faecal shedding of Campylobacter spp. A cohort of 133 heifers housed in four adjacent pens was examined over a five and a half month period, from entering the feedlot to slaughter. A parallel investigation of individual rectal faecal samples and pen environmental samples were taken at monthly intervals from November to February. The entire outer and inner surfaces of a carcass side of each animal were swabbed immediately following slaughter. Campylobacter spp. were isolated from 322 (54%) of the 600 rectal faecal samples. Campylobacter jejuni and C. coli accounted for 69 and 29.7% of the isolate recovered, respectively. A total of 159 environmental samples were examined, of these Campylobacter spp. was isolated from 46 samples (29%). Campylobacter jejuni and C. coli accounted for 35 and 59% of these isolates, respectively. Campylobacter spp. was not isolated from any of the dressed carcasses. Logistic regression indicated prevalence of Campylobacter spp. faecal shedding within pens was positively correlated to the pen, the month of sampling and the Campylobacter spp. contamination status of the pen dividing bars and the water trough surface. Campylobacter spp. should be considered as a pathogen shed in the faeces of a substantial proportion of feedlot cattle. However, with good hygienic practice during harvest, a very low level of this pathogen can be achieved on dressed carcasses.
Minihan
D
D
Department of Agriculture and Food, Abbotstown, Castleknock, Dublin 15, Ireland. donal.minihan@agriculture.gov.ie
Whyte
P
P
O'Mahony
M
M
Fanning
S
S
McGill
K
K
Collins
J D
JD
D000822:Animal Husbandry; D000818:Animals; D002167:Campylobacter / Q000145:classification / Q000302:isolation & purification* / Q000472:pathogenicity; D002169:Campylobacter Infections / Q000453:epidemiology / Q000635:transmission / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000382:microbiology / Q000635:transmission*; D015331:Cohort Studies; D018562:Disease Transmission, Infectious / Q000662:veterinary*; D005243:Feces / Q000382:microbiology; D005260:Female; D005516:Food Microbiology; D007494:Ireland / Q000453:epidemiology; D008137:Longitudinal Studies; D008460:Meat / Q000382:microbiology*; D015995:Prevalence; D012621:Seasons