Kinetics study of endogenous heat shock protein 70 expression.
The purpose of this study was to determine the kinetics of HSP70 expression in response to mild thermal stress. The rationale is to produce a basis for design of optimal heating methods to induce HSP70 expression for preconditioning in cardiac surgery. Bovine aortic endothelial cells were heated at 42 degrees C for 0.5 to 5 hours followed by 37 degrees C recovery for 1 to 48 hours. Quantitative analysis of western blot results showed HSP70 expression kinetics is a coupled function of heating temperature and time and of post-heating duration. Bimodal HSP70 expression kinetics were identified which may be an important cause of the "second window of protection" observed by other researchers.
Department of Biomedical Engineering, ENS612, 1 University Station C0800, University of Texas at Austin, Austin, TX 78712-0238, USA.
Sihong
S
Wang
Kenneth R
KR
Diller
Shanti J
SJ
Aggarwal
D000222:Adaptation, Physiological / Q000502:physiology / Q000528:radiation effects; D000818:Animals; D001011:Aorta / Q000378:metabolism / Q000528:radiation effects; D002417:Cattle; D002460:Cell Line; D003198:Computer Simulation; D004307:Dose-Response Relationship, Radiation; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000528:radiation effects*; D004735:Energy Transfer / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D018869:Heat-Shock Response / Q000502:physiology* / Q000528:radiation effects; D006358:Hot Temperature; D007700:Kinetics; D008954:Models, Biological; D011829:Radiation Dosage; D012062:Relative Biological Effectiveness
D018840:HSP70 Heat-Shock Proteins