Sorbitol uptake is regulated by glucose through the hexokinase pathway in vegetative peach-tree buds.
In peach trees (Prunus persica L. Batsch cv. Redhaven), sorbitol is a primary photosynthetic product and may play an important role in the budbreak process. Surprisingly, before budbreak (from January to early March), the concentration of sorbitol in the xylem sap decreases, while that of hexoses (glucose and fructose) increases. The aim of this work was to study the control of sorbitol uptake into vegetative buds by hexoses. Sorbitol uptake was selectively inhibited by hexoses at low and physiological concentrations and this effect was both reversible and concentration-dependent. In addition, the active uptake of sorbitol significantly declined in the plasma membrane vesicles-enriched fraction purified from glucose-treated vegetative buds, suggesting that the inhibitory action of glucose was at the membrane level. Finally, among several glucose analogues tested, only hexokinase substrates (2-deoxyglucose and mannose) were able to mimic the glucose effect, which was completely blocked by the hexokinase inhibitor mannoheptulose. These results represent the first steps towards a better understanding of polyol transport control in plants.
Maurel
Karine
K
UMR 547-PIAF Université Blaise Pascal, 24 avenue des Landais, F-63177 Aubière Cedex, France.
Sakr
Soulaïman
S
Gerbe
François
F
Guilliot
Agnès
A
Bonhomme
Marc
M
Rageau
Rémy
R
Pétel
Gilles
G
D001692:Biological Transport; D002250:Carbon Radioisotopes; D002462:Cell Membrane / Q000378:metabolism; D035263:Flowering Tops / Q000378:metabolism*; D005632:Fructose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006593:Hexokinase / Q000378:metabolism*; D010788:Photosynthesis; D027861:Prunus / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D012621:Seasons; D013012:Sorbitol / Q000378:metabolism*
D002250:Carbon Radioisotopes; D005632:Fructose; D013012:Sorbitol; D006593:Hexokinase; D005947:Glucose