Use of whey ultrafiltrate as a substrate for production of carotenoids by the yeast Rhodotorula rubra.
Carotenogenesis of the lactose-negative yeast Rhodotorula rubra GED5 was studied by cocultivation with Kluyveromyces lactis MP11 in whey ultrafiltrate (WU) (35, 50, and 70 g of lactose/L). Maximum yields of cell mass (24.3 g/L) and carotenoids (10.2 mg/L of culture fluid or 0.421 micro g/g of dry cells) were obtained by growing the microbial association in WU (50 g of lactose/L) in a fermentor with an airflow rate of 0.8 L/(L.min), agitation of 220 rpm, and temperature of 30 degrees C. The identified carotenoid pigments-beta-carotene, torulene, and torularhodin-reached maximum concentrations (133, 26.9, and 222.3 microg/g of dry cells, respectively) on d 5 for torulene and d 6 for beta-carotene and torularhodin.
Laboratory of Applied Microbiology, Institute of Microbiology, Bulgarian Academy of Sciences, 26 Maritza Boulevard, 4002 Plovdiv, Bulgaria. lbpmbas@plov.omega.bg
Ginka
G
Frengova
Emilina
E
Simova
Dora
D
Beshkova
D000818:Animals; D018533:Biomass; D002338:Carotenoids / Q000031:analogs & derivatives* / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D018920:Coculture Techniques / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods; D007716:Kluyveromyces / Q000254:growth & development / Q000378:metabolism*; D007785:Lactose / Q000032:analysis / Q000378:metabolism*; D008892:Milk / Q000737:chemistry* / Q000378:metabolism; D012248:Rhodotorula / Q000254:growth & development / Q000378:metabolism*; D014462:Ultrafiltration; D019207:beta Carotene / Q000096:biosynthesis / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D019207:beta Carotene; D002338:Carotenoids; C078922:torulene; D001616:beta-Galactosidase; D007785:Lactose; C078923:torularhodin