[Study on biology and epidemiological characteristics of vibrio cholerae non-O1 strains isolated from environmental waters in Foshan city].
OBJECTIVE
To study the distribution of serotype and the positive rate of toxins among vibrio cholerae non-O(1) isolated from environmental waters in Foshan city.


METHODS
Water specimens were collected from river and cultured for vibrio cholerae non-O(1). The PCR method was used to detect cholerae enterotoxin (CT) gene; the ELISA method was used to detect heat-stable toxin (ST) and heat-labile toxin (LT).


RESULTS
478 vibrio cholerae non-O(1) strains were isolated from 1 644 water specimens, with a positive rate of 29.07%. Serological assay showed that the main serotype of vibrio cholerae non-O(1) in Foshan city is VBO(7). Positive rate of CT, ST and LT were 1.91%, 13.14% and 12.17%, respectively.


CONCLUSIONS
A few non-O(1) strains were found to have several virulent factors simultaneously, and the results suggest that vibrio cholerae non-O(1) in environmental waters is potentially pathogenic and may affect people's health. It is necessary to pay attention to the prevention of diarrhoea caused by vibrio cholerae.
Center for Disease Control and Prevention of Foshan City, Guangdong Province, Foshan 528000, China.
Ai-zhen
AZ
Chen
Dai-song
DS
Cai
Su-yi
SY
Zhu
Zu-xing
ZX
Huang
Hua-le
HL
Liao
Guo-you
GY
Yan
Wen
W
Zhu
D002681:China; D004768:Enterotoxins / Q000235:genetics; D004784:Environmental Monitoring / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D016133:Polymerase Chain Reaction; D012621:Seasons; D012703:Serotyping; D044163:Vibrio cholerae non-O1 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014867:Water / Q000032:analysis*; D014871:Water Microbiology
D004768:Enterotoxins; C037420:stN protein, Vibrio cholerae; D014867:Water