Delineation of a 2.8 megabases region harboring a potential tumor suppressor gene involved in renal cell carcinoma, that is commonly deleted from chromosome 14.
METHODS
To investigate the genetic alterations that occur during the development of renal cell carcinomas (RCC), we used 20 microsatellite markers to examine 48 renal cell carcinomas for allelic losses of chromosome arm 14q.


RESULTS
We identified 14q LOH in 31% of cases. Twelve tumors were entirely lacking the 14q arm and three were partially deleted. For the first time on fresh tumors, these findings led to the delineation of a 17.9 Mb region between markers D14S281 and D14S277 that is commonly deleted. Interestingly, this segment overlaps with the previously reported 37.8 Mb commonly deleted region.


CONCLUSIONS
Taken together these results allowed us to define a new 2.8 Mb segment between markers D14S588 and D14S277 that potentially harbors a tumor suppressor gene involved in the development of RCC which can be reached by positional cloning.
Gallou
Catherine
C
INSERM U383, Hôpital Necker-Enfants Malades, Clinique Maurice LAMY, 149 Rue de Sèvres, 75745 Paris, France. catherine.gallou@necker.fr
Méjean
Arnaud
A
Bouvier
Raymonde
R
Lucien
Florence
F
Perennou
Morgan
M
Zindy
Pierre-Joachim
PJ
Grifone
Raphaelle
R
Chrétien
Yves
Y
Junien
Claudine
C
Béroud
Christophe
C
D000328:Adult; D000368:Aged; D002292:Carcinoma, Renal Cell / Q000235:genetics*; D002883:Chromosomes, Human, Pair 14; D005260:Female; D016147:Genes, Tumor Suppressor; D005819:Genetic Markers; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D019656:Loss of Heterozygosity / Q000235:genetics*; D008297:Male; D008875:Middle Aged
D005819:Genetic Markers