Cloning and characterization of the promoter region of human LPTS/PinX1 gene.
The gene for LPTS/PinX1 encodes a potent telomerase inhibitor and suppresses tumor cell growth. In order to investigate the transcriptional regulation of this gene, we isolated its 5'-flanking region from the human genomic BAC clone and identified a major transcriptional initiation site. The sequence of the 5'-flanking region is GC-rich, lacks canonical TATA box, but contains potential binding sites for a variety of transcription factors. The deletion analysis indicated that the proximal 100 bp (from nt -66 to +34) is essential for minimal promoter activity and the regions of promoter from nt -1272 to -573 and nt -330 to -66 are required for maximal expression of the LPTS/PinX1 gene. Four DNase I hypersensitive sites (DHS1-4) mapping to the regions of transcription initiation and promoter in LPTS/Pinx1 gene were also revealed.
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China.
Suiquan
S
Wang
Cheng
C
Liao
Tsaiping
T
Li
Mujun
M
Zhao
D001483:Base Sequence; D001665:Binding Sites; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular; D003850:Deoxyribonuclease I / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D017421:Sequence Analysis; D024363:Transcription Initiation Site; D025521:Tumor Suppressor Proteins / Q000737:chemistry / Q000235:genetics*
D018797:Cell Cycle Proteins; C440808:PINX1 protein, human; D011506:Proteins; D025521:Tumor Suppressor Proteins; D003850:Deoxyribonuclease I