Glutamatergic transmission in the rat brain and gravitational stress.
The effects of hypergravity stress on L-[14C]-glutamate release from synaptosomes obtained from the rat brain and on the kinetic parameters of high-affinity glutamate transport activity were investigated. We found that hypergravity stress affected only the Ca(2+)-dependent component of L-[14C]-glutamate release. It did not modify the transporter affinity, but the maximum rate of uptake dropped from 12.5 +/- 3.2 to 5.6 +/- 0.9 nmol/min/mg of protein (in control rats and in animals subjected to hypergravity, respectively).
Borisova
T A
TA
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine. tborisov@paladin.biochem.kiev.ua
Krisanova
N V
NV
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000502:physiology*; D002498:Centrifugation; D003470:Culture Media; D018698:Glutamic Acid / Q000493:pharmacokinetics*; D018471:Hypergravity; D011189:Potassium Chloride / Q000493:pharmacokinetics; D051381:Rats; D013574:Synaptosomes / Q000378:metabolism*
D003470:Culture Media; D018698:Glutamic Acid; D011189:Potassium Chloride; D002118:Calcium