Colorimetric detection of protein microarrays based on nanogold probe coupled with silver enhancement.
This work presents a method for analyzing protein microarrays using a colorimetric nanogold probe coupled with silver enhancement (gold-silver detection). In this method, the gold nanoparticles were introduced to the microarray by the specific binding of the gold-conjugated antibodies or streptavidins and then coupled with silver enhancement to produce black image of microarray spots, which can be easily detected with a commercial CCD camera. The method showed high detection sensitivity (1 pg of IgG immobilized on slides or 2.75 ng/ml IgG in solution) and a good linear correlation between the signal intensity and the logarithm of the sample concentration. The examination of this method in analyzing a demonstrational ToRCH antigen microarray developed in our lab showed an identical result as in the fluorescent method. These results suggest the colorimetric gold-silver detection method has potential applications in proteomics research and clinical diagnosis.
Liang
Ru-Qiang
RQ
Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China.
Tan
Cui-Yan
CY
Ruan
Kang-Cheng
KC
D000818:Animals; D003124:Colorimetry / Q000379:methods*; D006046:Gold; D006801:Humans; D040081:Protein Array Analysis; D012680:Sensitivity and Specificity; D012834:Silver
D012834:Silver; D006046:Gold