Detection of Cryptosporidium parvum in soil extracts.
Epifluorescent microscopy and flow cytometry were used in different combinations with fluorescein isothiocyanate-labeled immunoglobulins M and G3 to estimate the numbers of Cryptosporidium parvum oocysts in soil extracts containing 10 to 10,017 oocysts/ml. No combination had a systematic effect on accuracy or precision. Background debris may have produced overestimates at low oocyst concentrations when flow cytometry was used.
Walker
Mark
M
Department of Natural Resources and Environmental Sciences, University of Nevada, Reno, Nevada 89557, USA. mwalker@equinox.unr.edu
Redelman
Douglas
D
D000818:Animals; D000913:Antibodies, Protozoan; D016785:Cryptosporidium parvum / Q000276:immunology / Q000302:isolation & purification*; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D008856:Microscopy, Fluorescence; D033661:Oocysts / Q000302:isolation & purification; D012987:Soil / Q000469:parasitology*
D000913:Antibodies, Protozoan; D005456:Fluorescent Dyes; D012987:Soil; D016650:Fluorescein-5-isothiocyanate