The differentiation of early embryonic stem cells into adipocytes-like cells.
An early embryonic stem cell line, EES-6 cells, was established from 2-cell stage embryos of ddY mice. The cells were maintained in an undifferentiated state with D-MEM/F12 medium supplemented with 10% fetal bovine serum (FBS) (GM) and 1 ng of leukemia inhibitory factor (LIF) without any feeder cells. In this study, EES cells were cultured with a medium containing embryotrophic factors (ETFs) which promoted the differentiation of EES cells into white and brown adipocytes-like cells for a period of 5 days. Lipid droplets in brown adipocyte-like cells were stained with Sudan III; however, large lipid-like droplets in white or brown adipocyte-like cells were unstained with either Sudan III or alcian blue. These findings have numerous possibilities for therapeutic use such as regeneration of skin and wound healing.
Dept of Anatomy, The Jikei University School of Medicine, Tokyo, Japan.
Motoyoshi
M
Yamamoto
Toshiaki
T
Tachibana
Hisashi
H
Hashimoto
Isamu
I
Ishiwata
Hiroshi
H
Ishikawa
D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D003470:Culture Media; D004622:Embryo, Mammalian / Q000166:cytology*; D015850:Interleukin-6 / Q000494:pharmacology; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D051379:Mice; D008815:Mice, Inbred Strains; D018121:Receptors, Cytokine / Q000502:physiology; D053606:Receptors, OSM-LIF; D044968:Regenerative Medicine; D015398:Signal Transduction / Q000187:drug effects; D013234:Stem Cells / Q000166:cytology*
D003470:Culture Media; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; C507838:Lif protein, mouse; C507841:Lifr protein, mouse; D018121:Receptors, Cytokine; D053606:Receptors, OSM-LIF