Strategies for generation of an siRNA expression library directed against the human genome.
RNA interference (RNAi) is a phenomenon whereby expression of an individual gene is specifically silenced by the introduction of a double-stranded RNA (dsRNA) whose sequence is homologous to that of the gene in question. The generation of a small interfering RNA (siRNA) expression library directed against the entire human genome is a project that requires solutions to many difficult technical problems. We present here some strategies for solving some of these problems, including the development of genetically stable and highly active siRNA expression vectors, a procedure for selection of favorable target sites, and an efficient and inexpensive procedure for constructing an siRNA expression library.
Miyagishi
Makoto
M
Department of Chemistry and Biotechnology, School of Engineering, University of Tokyo, Tokyo 113-8656, Japan.
Taira
Kazunari
K
D000465:Algorithms; D001483:Base Sequence; D015723:Gene Library; D020868:Gene Silencing; D005821:Genetic Techniques; D015894:Genome, Human; D006367:HeLa Cells; D006801:Humans; D009690:Nucleic Acid Conformation; D011237:Predictive Value of Tests; D011401:Promoter Regions, Genetic; D012330:RNA, Double-Stranded / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000737:chemistry / Q000235:genetics*; D014162:Transfection / Q000379:methods
D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D034741:RNA, Small Interfering