Construction of an electro-enzymatic bioreactor for the production of (R)-mandelate from benzoylformate.
Coupling both the electrocatalytic recycling of NADH and the enzymatic reduction of the substrate was used to produce (R)-mandelate from benzoylformate using benzoylformate reductase (BFR). The reduction of benzoylformate by BFR in combination with FAD-mediated electrolysis (at -0.5 V vs. Ag/AgCl) was complete in about 18 h and gave 47.5 mM (R)-mandelate from 50 mM substrate, while the process involving MV2+-mediated procedure (at -0.7 V vs. Ag/AgCl) produced 40 mM (R)-mandelate after 30 h. The overpotential for the NAD+ reduction could be decreased by about 0.2 V by substituting a toxic viologen derivative, MV2+, with a natural electron carrier, FAD. MV2+, however, decreased the productivity as BFR lost about 50% of its initial activity after 6 d in its presence.
Department of Food Science and Technology and Institute of Agricultural Science and Technology, Chonbuk National University, Chonju 561-756, Korea.
Mee-Hae
MH
Kim
Sei-Eok
SE
Yun
D019149:Bioreactors; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004572:Electrolysis / Q000295:instrumentation* / Q000379:methods; D004574:Electromagnetic Fields; D013293:Enterococcus faecalis / Q000201:enzymology*; D004789:Enzyme Activation; D004867:Equipment Design; D004868:Equipment Failure; D005240:Feasibility Studies; D006038:Glyoxylates / Q000737:chemistry*; D008333:Mandelic Acids / Q000138:chemical synthesis*; D009243:NAD / Q000737:chemistry* / Q000528:radiation effects; D010088:Oxidoreductases / Q000737:chemistry* / Q000528:radiation effects
D006038:Glyoxylates; D008333:Mandelic Acids; D009243:NAD; C012482:phenylglyoxylic acid; D010088:Oxidoreductases; C037938:mandelic acid