[Effect of nandrolone phenylpropionate on hepatic albumin mRNA and granulational alpha 1(I) procollagen mRNA in burned rats].
OBJECTIVE
To assess the mechanism for the effect of nandrolone phenypropionate (NP) on hepatic albumin mRNA and granulational alpha 1(I) procollagen mRNA in burned rats with the aim to underpin the clinical application of anabolic steroids.


METHODS
Thirty-two Wistar rats with a deep second-degree cutaneous burn of 20% total body surface area were randomly divided into two groups to receive either 5 mg/kg NP (NP group) or normal saline as placebo(control group) every other day. The expression copy quantities of albumin-mRNA in liver tissue and alpha 1 (I) procollagen mRNA in granulation wound were measured by quantitative fluorescent RT-PCR respectively on the post-burned days 4, 7, 14 and 21.


RESULTS
The expression levels of albumin-mRNA and alpha 1 (I) procollagen mRNA in NP group were much higher than those in control group. The 7th and 14th days were the periods in which the albumin-mRNA and alpha 1 (I) procollagen mRNA expression had been increasing obviously (P < 0.01).


CONCLUSIONS
Nandrolone phenylpropionate could effectively up-regulate the expression of albumin-mRNA in liver tissue and the alpha 1 (I) procollagen mRNA in granulation wound.
Department of Burn and Plastic Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Ying
Y
Cen
Kai
K
Li
Ning
N
Liu
Xiao-xue
XX
Liu
D000418:Albumins / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D002056:Burns / Q000378:metabolism*; D024042:Collagen Type I / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D008099:Liver / Q000378:metabolism*; D008297:Male; D009277:Nandrolone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014945:Wound Healing / Q000187:drug effects
D000418:Albumins; D024042:Collagen Type I; D012333:RNA, Messenger; D009277:Nandrolone; C008580:nandrolone phenpropionate