[The effect of basic fibroblast growth factor on angiogenesis and on the expression of basic fibroblast growth factor and vascular endothelial growth factor in acute infarcted myocardium].
OBJECTIVE
To evaluate the effect of intramyocardial administration of basic fibroblast growth factor (bFGF) on the angiogenesis and on the expression of bFGF and vascular endothelial growth factor (VEGF) in infarcted myocardium.


METHODS
Twenty-four mongrel dogs were randomized into control group and therapeutic group. Acute myocardial infarction was made by ligation of the left anterior descending coronary artery distal to its first diagonal branch. After coronary artery was occlused, 50 micrograms of basic fibroblast growth factor in 15 ml of normal saline were injected into the infarcted myocardium and its border zone in therapeutic group, whereas the same volume of normal saline alone was used in control dogs. Every 3 dogs in each group was studied on the 1st day, the 3rd day, the 10th day and the 17th day after surgery respectively. With sensitivity encoded technique, cine MR was performed on each dog before euthanasia to evaluate the cardiac function. Angiogenesis and the expression of VEGF and bFGF were evaluated by immunohistochemical studies with factor VIII and the antibodies of VEGF and bFGF.


RESULTS
In therapeutic group, left ventricular ejection fraction, improved markedly since the 10th day after injection of bFGF. Microvessel density was higher in the therapeutic group than in the control group except on the first day. VEGF and bFGF expressed more in the therapeutic group.


CONCLUSIONS
Intramyocardial administration of bFGF is useful in increasing the growth of microvessels and improving the left ventricular function in acute myocardial infarction.
Department of Radiology, Xijing Hospital, Fourth Military University, Xi'an 710032, China.
Ying
Y
Liu
Li-jun
LJ
Sun
Yi
Y
Huan
Hai-tao
HT
Zhao
Ya-li
YL
Ge
Fa-bao
FB
Gao
D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism / Q000627:therapeutic use*; D007150:Immunohistochemistry; D019028:Magnetic Resonance Imaging, Cine / Q000379:methods; D008297:Male; D009203:Myocardial Infarction / Q000188:drug therapy* / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D013997:Time Factors; D016896:Treatment Outcome; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2