Evaluation of a new generation of plastic culture bottles with an automated microbial detection system for nine common contaminating organisms found in PLT components.
BACKGROUND
A microbial detection system (BacT/ALERT 3D, bioMérieux [formerly Organon Teknika]) has previously been validated with a variety of bacterial contaminants in PLTs. The recovery of nine organisms seeded into PLTs with new plastic culture bottles was studied in comparison to the current glass bottles. The use of plastic instead of glass would be expected to reduce the risk of injury.


METHODS
Isolates of Bacillus cereus, Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Serratia marcescens, Streptococcus viridans, and Propionibacterium acnes were inoculated into Day 2 (>24 hr <48 hr) apheresis PLT units to 10 and 100 CFUs per mL. Replicate samples (4 mL) were inoculated into both current- and new-generation standard aerobic and anaerobic bottles.


RESULTS
All organisms (with the exception of P. acnes) were detected in a mean time of 9.3 to 18.9 hours (10 CFUs/mL) or 8.7 to 18.2 hours (100 CFUs/mL). In aggregate (with the exception of P. acnes), the plastic and glass aerobic bottles had a mean difference in detection of 1.2 hours (p < 0.0001), and the plastic and glass anaerobic bottles had a mean difference of 3.3 hours (p < 0.0001). In all cases, the mean detection time was superior or clinically comparable (within 0.1 hr) with the new plastic bottles. P. acnes (an anaerobic organism) was detected with the new and current anaerobic bottles in a mean of 72.8 and 90.4 hours (10 CFUs/mL) or 64.0 and 80.8 hours (100 CFUs/mL), respectively. The narrower bottle neck and smaller inoculation septum present with the new-generation plastic bottles were inoculated with comparable ease to that of the glass bottles.


CONCLUSIONS
These data demonstrate that the new plastic bottles are clinically comparable or superior to the current glass standard aerobic and anaerobic culture bottles.
Brecher
M E
ME
Transfusion Medicine Service, CB 7600, University of North Carolina Hospitals, 101 Manning Drive, Chapel Hill, NC 27514, USA. Brecher@med.unc.edu
Hay
S N
SN
Rothenberg
S J
SJ
D001409:Bacillus cereus / Q000302:isolation & purification; D001424:Bacterial Infections / Q000517:prevention & control*; D001781:Blood Component Removal; D001792:Blood Platelets; D016972:Enterobacter cloacae / Q000302:isolation & purification; D004926:Escherichia coli / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D005898:Glass; D006801:Humans; D007711:Klebsiella pneumoniae / Q000302:isolation & purification; D008828:Microbiological Techniques / Q000295:instrumentation / Q000379:methods; D010969:Plastics; D017713:Platelet Transfusion; D011425:Propionibacterium acnes / Q000302:isolation & purification; D012706:Serratia marcescens / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000302:isolation & purification; D013212:Staphylococcus epidermidis / Q000302:isolation & purification; D034363:Viridans Streptococci / Q000302:isolation & purification
D010969:Plastics