Control of cell adhesion and detachment using temperature and thermoresponsive copolymer grafted culture surfaces.
The hydrophobic monomer, n-butyl methacrylate (BMA) has been incorporated into thermoresponsive poly(N-isopropylacrylamide) (PIPAAm) to lower PIPAAm phase transition temperatures necessary for systematically regulating cell adhesion on and detachment from culture dishes at controlled temperatures. Poly(IPAAm-co-BMA)-grafted dishes were prepared by electron beam irradiation methods, systematically changing BMA content in the feed. Copolymer-grafted surfaces decreased grafted polymer transition temperatures with increasing BMA content as shown by water wettabilities compared to homopolymer PIPAAm-grafted surfaces. Bovine endothelial cells readily adhered and proliferated on copolymer-grafted surfaces above collapse temperature at 37 degrees C, finally reaching confluence. Cell sheet detachment behavior from copolymer-grafted surfaces depended on the culture temperature and BMA content. In conclusion, cell attachment/detachment can be controlled to an arbitrary temperature by varying the content of hydrophobic monomer incorporated into PIPAAm grafted to culture surfaces.
Graduate School of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku, Tokyo 169-8555, Japan.
Yukiko
Y
Tsuda
Akihiko
A
Kikuchi
Masayuki
M
Yamato
Yasuhisa
Y
Sakurai
Mitsuo
M
Umezu
Teruo
T
Okano
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002417:Cattle; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002455:Cell Division; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology; D057927:Hydrophobic and Hydrophilic Interactions; D008567:Membranes, Artificial; D011109:Polymethacrylic Acids / Q000737:chemistry; D013696:Temperature
D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011109:Polymethacrylic Acids