Elevated plasma human urotensin-II-like immunoreactivity in ischemic cardiomyopathy.
BACKGROUND
The recently discovered, vasoactive, cyclic undecapeptide human urotensin-II (hU-II), and its G-protein coupled receptor (GPR14) are both expressed in the human cardiovascular system. Little is known about the pathophysiological relevance of hU-II. We hypothesised that circulating hU-II is elevated in patients with coronary artery disease (CAD) corresponding to the degree of cardiac dysfunction.


METHODS
38 patients were diagnosed with coronary artery disease by left heart catheterization, and their functional status was classified according to the New York Heart Association (NYHA). hU-II-like immunoreactivity (hU-II-LI) was measured using a novel specific and sensitive enzyme-linked immunoassay. Calculations were performed with log-transformed hU-II-LI values.


RESULTS
hU-II-LI correlated positively with left ventricular end diastolic pressure (LVEDP) (r=0.32, P=0.05) and tended to correlate inversely with left ventricular ejection fraction (LV-EF) (r=-0.31, P=0.061). There was a positive correlation between hU-II-LI and NYHA class (r=0.53, P=0.001). Circulating hU-II-LI was significantly higher in patients with NYHA class III (4822+/-723 pg/ml, N=13) than in patients with class I (1884+/-642 pg/ml, N=9, P=0.007) or class II (2294+/-426 pg/ml, N=15, P=0.046). There was no difference between classes I and II (P=0.83). Furthermore, hU-II-LI correlated significantly with B-type natriuretic peptide, a marker for heart failure (r=0.40, P=0.025). In a linear regression analysis, NYHA class was the only significant independent predictor of hU-II-LI.


CONCLUSIONS
The present study demonstrates that plasma hU-II-LI rises significantly in proportion to parameters of cardiac dysfunction and functional impairment in patients with coronary artery disease. These results suggest a pathophysiological role for hU-II in cardiac disease and warrant further investigation.
Lapp
Harald
H
Herzzentrum Wuppertal, Helios-Klinikum, Wuppertal, Germany. hlapp@wuppertal.helios-kliniken.de
Boerrigter
Guido
G
Costello-Boerrigter
Lisa C
LC
Jaekel
Karsten
K
Scheffold
Thomas
T
Krakau
Ingo
I
Schramm
Matthias
M
Guelker
Hartmut
H
Stasch
Johannes-Peter
JP
D015415:Biomarkers / Q000097:blood; D002311:Cardiomyopathy, Dilated / Q000097:blood / Q000503:physiopathology*; D016000:Cluster Analysis; D003324:Coronary Artery Disease / Q000503:physiopathology; D005260:Female; D006321:Heart / Q000503:physiopathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D014579:Urotensins / Q000097:blood*
D015415:Biomarkers; D014579:Urotensins; C029677:urotensin II