Brain IL-1 beta was involved in reserpine-induced behavioral depression in rats.
OBJECTIVE
To investigate the mechanism of brain interleukin-1 beta (IL-1 beta) in reserpine-induced behavioral depression in rats.


METHODS
Porsult swim test was used in the measurement of depressive behavior and ELISA was used in measurement of brain IL-1 beta.


RESULTS
Intraperitoneal injection of reserpine (0, 4, 6, and 8 mg/kg, ip) increased floating time in the Porsult swim test in a dose-and time-dependent manner in rats. Intracerebroventricular injection (icv) of IL-1 beta receptor antagonist (IL-1ra, 6 mg/kg) blocked the increment of floating time in Porsult swim test at 48 and 72 h after reserpine injection, but not at 1 and 24 h after injection. Brain IL-1 beta increased after reserpine treatment in posterior cortex, hippocampus, and hypothalamus. The increase of IL-1 beta concentration starts at 24 hours after injection of reserpine and reached the peak at 48 h.


CONCLUSIONS
Reserpine induced behavioral depression partially via brain interleukin-1 beta generation.
Huang
Qing-jun
QJ
Psychoneuroimmunolgy Laboratory, Bethune Military Medical College, Shijiazhuang 050081, China. stone89@heinfo.net
Jiang
Hong
H
Hao
Xin-ling
XL
Minor
Thomas R
TR
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D003863:Depression / Q000139:chemically induced / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D007276:Injections, Intraventricular; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors; D012110:Reserpine; D012795:Sialoglycoproteins / Q000494:pharmacology*
D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D017472:Receptors, Interleukin-1; D012795:Sialoglycoproteins; D012110:Reserpine