Detection of murine intestinal adenomas using targeted molecular autofluorescence.
There is a significant need for noninvasive methods of evaluating dysplastic and neoplastic lesions in the luminal gastrointestinal tract. We have optimized an approach based on autofluorescence to study dysplastic adenomatous polyps in Apc(min/+) mice. We made recordings from both normal intestinal mucosa and from polyps using a xenon lamp-based fiberoptic device. Seventy-eight polyps in 11 mice revealed an increase in mean autofluorescence intensity ratios of 1.29 +/- 0.04 (72 small intestinal polyps; P < 0.0001) and 1.28 +/- 0.05 (6 colon polyps; P = 0.0016). Serial measurements of autofluorescence discriminated polyps from normal mucosa with a sensitivity, verified histologically, of 95%. To understand the chemical basis for increased autofluorescence, we examined the tryptophan content of intestinal polyps and the adjacent normal mucosa in a small subset of animals. The findings revealed an increased concentration of tryptophan in polyps (990 +/- 240 ng/mg) compared to normal mucosa (720 +/- 150 ng/mg; P = 0.03). In conclusion, these findings suggest that autofluorescence intensity increases in the setting of intestinal neoplasia and can be used to detect adenomas in the mouse intestine in real time.
Division of Gastroenterology, Department of Medicine, Washington University, Saint Louis, Missouri 63110, USA. bbanerje@im.wustl.edu
Bhaskar
B
Banerjee
Jeffrey O
JO
Henderson
Thomas C
TC
Chaney
Nicholas O
NO
Davidson
D018256:Adenomatous Polyps / Q000175:diagnosis*; D000818:Animals; D003111:Colonic Polyps / Q000139:chemically induced; D004195:Disease Models, Animal; D007417:Intestinal Polyps / Q000175:diagnosis*; D007421:Intestine, Small; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D010865:Pilot Projects; D011230:Precancerous Conditions / Q000175:diagnosis; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D014364:Tryptophan / Q000378:metabolism
D014364:Tryptophan