Epiblast cells that express MyoD recruit pluripotent cells to the skeletal muscle lineage.
Embryonic stem cells are derived from the epiblast. A subpopulation of epiblast cells expresses MyoD mRNA and the G8 antigen in vivo. G8 positive (G8pos) and G8 negative (G8neg) populations were isolated by magnetic cell sorting. Nearly all G8pos cells switched from E- to N-cadherin and differentiated into skeletal muscle in culture. G8neg cells were impaired in their ability to switch cadherins and few formed skeletal muscle. Medium conditioned by G8pos cells stimulated skeletal myogenesis and N-cadherin synthesis in G8neg cultures. The effect of conditioned medium from G8pos cultures was inhibited by bone morphogenetic protein (BMP) 4. Treatment of G8neg cells with a soluble form of the BMP receptor-IA or Noggin promoted N-cadherin synthesis and skeletal myogenesis. These results demonstrate that MyoD-positive epiblast cells recruit pluripotent cells to the skeletal muscle lineage. The mechanism of recruitment involves blocking the BMP signaling pathway.
Department of Anatomy, Philadelphia College of Osteopathic Medicine, 4170 City Ave., Philadelphia, PA 19131, USA.
Jacquelyn
J
Gerhart
Christine
C
Neely
Benjamin
B
Stewart
Jordanna
J
Perlman
David
D
Beckmann
Margaretha
M
Wallon
Karen
K
Knudsen
Mindy
M
George-Weinstein
D000818:Animals; D015415:Biomarkers; D055415:Bone Morphogenetic Protein 4; D052005:Bone Morphogenetic Protein Receptors, Type I; D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism; D002352:Carrier Proteins; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage; D002478:Cells, Cultured; D002642:Chick Embryo / Q000033:anatomy & histology* / Q000502:physiology; D017077:Culture Media, Conditioned; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004848:Epithelium / Q000033:anatomy & histology / Q000502:physiology*; D018189:Immunomagnetic Separation; D018482:Muscle, Skeletal / Q000166:cytology / Q000196:embryology*; D017570:MyoD Protein / Q000235:genetics / Q000378:metabolism*; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D017978:Receptors, Growth Factor / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism; D051176:beta Catenin; D000099176:Noggin Protein
D015415:Biomarkers; D055415:Bone Morphogenetic Protein 4; D052005:Bone Morphogenetic Protein Receptors, Type I; D019485:Bone Morphogenetic Proteins; D015820:Cadherins; D002352:Carrier Proteins; D017077:Culture Media, Conditioned; D003598:Cytoskeletal Proteins; D017570:MyoD Protein; D017346:Protein Serine-Threonine Kinases; D011506:Proteins; D017978:Receptors, Growth Factor; D015534:Trans-Activators; D051176:beta Catenin; D000099176:Noggin Protein