A model to study intestinal and hepatic metabolism of propranolol in the dog.
A model to investigate hepatic drug uptake and metabolism in the dog was developed for this study. Catheters were placed in the portal and hepatic veins during exploratory laparotomy to collect pre- and posthepatic blood samples at defined intervals. Drug concentrations in the portal vein were taken to reflect intestinal uptake and metabolism of an p.o. administered drug (propranolol), while differences in drug and metabolite concentrations between portal and hepatic veins reflected hepatic uptake and metabolism. A significant difference in propranolol concentration between hepatic and portal veins confirmed a high hepatic extraction of this therapeutic agent in the dog. This technically uncomplicated model may be used experimentally or clinically to determine hepatic function and metabolism of drugs that may be administered during anaesthesia and surgery.
School of Veterinary Science Therapeutics Research Unit, Southern Clinical Division, University Of Queensland, Brisbane, Queensland 4072, Australia. p.mills@mailbox.uq.edu.au
P C
PC
Mills
G A
GA
Siebert
M S
MS
Roberts
D000284:Administration, Oral; D000319:Adrenergic beta-Antagonists / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D000818:Animals; D002404:Catheterization / Q000662:veterinary; D004285:Dogs / Q000378:metabolism*; D005260:Female; D006503:Hepatic Veins; D007413:Intestinal Mucosa / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008954:Models, Biological; D011169:Portal Vein; D011433:Propranolol / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*
D000319:Adrenergic beta-Antagonists; D011433:Propranolol