[Cyclin D2 expression in chronic myelogenous leukemia].
OBJECTIVE
To investigate the relationship between cyclin D2 and P210(BCR/ABL) tyrosine kinase in chronic myelogenous leukemia (CML).


METHODS
RT-PCR, Western blot and flow cytometry were performed to detect the expression of cyclin D2 in K562 cells and in K562-ib-eGFP cells which express intracellular single-chain antibody (sFv, intrabody) against ABL tyrosine kinase domain.


RESULTS
Cyclin D2 expression in K562-ib-eGFP cells was 18.90% which was lower than that of control K562 cells (48.10%), and the number of S-phase cells in K562-ib-eGFP was 40.40% which was much lower than that in K562 cells (64.34%).


CONCLUSIONS
Cyclin D2 is a potential down-stream signal molecule of the p210(BCR/ABL) tyrosine kinase in CML. The altered expression of cyclin D2 may contribute to the over proliferation of CML cells.
State Key Laboratory of Experimental Hematology, Institute of Hematology, CAMS & PUMC, Tianjin 300020, China.
Jun-min
JM
Song
Dong
D
Xu
Er-jin
EJ
Fan
Shi-rong
SR
Xu
Dong
D
Li
Chun-hua
CH
Zhao
D015153:Blotting, Western; D056742:Cyclin D2; D016213:Cyclins / Q000032:analysis* / Q000235:genetics; D005434:Flow Cytometry; D016313:Genes, abl; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
C080336:CCND2 protein, human; D056742:Cyclin D2; D016213:Cyclins