Generation of reelin-positive marginal zone cells from the caudomedial wall of telencephalic vesicles.
An early and fundamental step of the laminar organization of developing neocortex is controlled by the developmental programs that critically depend on the activities of reelin-positive cells in the marginal zone. However, the ontogeny of reelin-positive cells remained elusive. To gain insights into the spatial and temporal regulation of reelin-positive marginal zone cell development, we used a transgenic mouse line in which we defined the green fluorescent protein (GFP) transgene as a novel reliable molecular marker of reelin-positive marginal zone cells from the early stages of their development. We further used exo utero electroporation-mediated gene transfer that allows us to mark progenitor cells and monitor the descendants in the telencephalon in vivo. We show here the generation of reelin-positive marginal zone cells from the caudomedial wall of telencephalic vesicles, including the cortical hem, where the prominent expression of GFP is initially detected. These neurons tangentially migrate at the cortical marginal zone and are distributed throughout the entire neocortex in a caudomedial-high to rostrolateral-low gradient during the dynamic developmental period of corticogenesis. Therefore, our findings on reelin-positive marginal zone cells, in addition to the cortical interneurons, add to the emerging view that the neocortex consists of neuronal subtypes that originate from a focal source extrinsic to the neocortex, migrate tangentially into the neocortex, and thereby underlie neural organization of the neocortex.
Translational Research Department, Molecular Bio-Medicine Unit, Japan Science and Technology, Mitsubishi Kagaku Institute of Life Sciences, Machida, Tokyo, 194-8511, Japan.
Keiko
K
Takiguchi-Hayashi
Mariko
M
Sekiguchi
Shizuko
S
Ashigaki
Masako
M
Takamatsu
Hiroshi
H
Hasegawa
Rika
R
Suzuki-Migishima
Minesuke
M
Yokoyama
Shigetada
S
Nakanishi
Yasuto
Y
Tanabe
D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis; D015816:Cell Adhesion Molecules, Neuronal / Q000096:biosynthesis*; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D018274:Electroporation; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis*; D017930:Genes, Reporter; D016147:Genes, Tumor Suppressor; D005865:Gestational Age; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D019579:Neocortex / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D009419:Nerve Tissue Proteins; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009687:Nuclear Proteins / Q000096:biosynthesis; D000091042:Reelin Protein; D012697:Serine Endopeptidases; D013687:Telencephalon / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins
D000943:Antigens, Differentiation; D015816:Cell Adhesion Molecules, Neuronal; D004268:DNA-Binding Proteins; D016326:Extracellular Matrix Proteins; D008164:Luminescent Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D000091042:Reelin Protein; C000606368:Trp73 protein, mouse; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins; D049452:Green Fluorescent Proteins; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases