Sub-second isoelectric focusing in free flow using a microfluidic device.
Using a microfabricated chip with a bed volume of 0.2 microL we demonstrate the validity of the scaling laws for molecular mass transport of isoelectric focusing (IEF) in free flow. Nano- or microlitre sample volumes can be concentrated within 430 ms by a factor of up to 400. These very fast performances make the chip applicable to proteomic analysis and for continuous monitoring of biochemical processes.
Imperial College London, Chemistry Department, Exhibition Road, London, UK SW7 2AZ.
Yi
Y
Xu
Chao-Xuan
CX
Zhang
Dirk
D
Janasek
Andreas
A
Manz
D000803:Angiotensin I / Q000032:analysis; D000804:Angiotensin II / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D007525:Isoelectric Focusing / Q000295:instrumentation*; D008838:Microcomputers; D036103:Nanotechnology; D010455:Peptides / Q000737:chemistry
D010455:Peptides; D000804:Angiotensin II; D007334:Insulin-Like Growth Factor I; D000803:Angiotensin I