Autologous transplantation of peripheral blood stem cells as an effective therapeutic approach for severe arteriosclerosis obliterans of lower extremities.
Treatment of severe arteriosclerosis obliterans of lower extremities (ASOLE) remains a clinical challenge. To develop a more effective approach, we evaluated the clinical efficacy of autologous transplantation of mobilized peripheral blood stem cells (PBSCs) in 5 patients with ASOLE. The patients received recombinant human granulocyte colony-stimulating factor (rhG-CSF, 600 micro g/day) for 5 consecutive days. On day 5, PBSCs were collected, sorted from blood circulation of patients, and then intramuscularly injected into their ischemic lower limbs. A significant improvement of clinical manifestations including severe pain, skin temperature and ulcer, was observed, without obvious adverse effect. The patient's limb was successfully saved. Satisfactory remission was obtained 3 months after transplantation as shown by significant improvement in ankle-brachial pressure index (ABI), blood flow in personal vascular laboratory (PVL), laser Doppler blood perfusion, and the angio-graphic scores. Our data suggest for the first time that autologous transplantation of mobilized PBSCs provides a practical, safe, and effective method of treatment for lower limb ischemia.
National Research Center for Stem Cell Engineering and Technology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China. tihzchan@public.tpt.tj.cn
Ping Ping
PP
Huang
Shan Zhu
SZ
Li
Ming Zhe
MZ
Han
Zhi Jian
ZJ
Xiao
Ren Chi
RC
Yang
Lu Gui
LG
Qiu
Zhong Chao
ZC
Han
D000368:Aged; D000792:Angiography; D001162:Arteriosclerosis Obliterans / Q000628:therapy*; D001831:Body Temperature; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000627:therapeutic use; D006801:Humans; D017078:Laser-Doppler Flowmetry; D035002:Lower Extremity / Q000473:pathology*; D008875:Middle Aged; D010146:Pain; D010477:Perfusion; D011994:Recombinant Proteins / Q000627:therapeutic use; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology; D013997:Time Factors; D014182:Transplantation, Autologous / Q000379:methods*
D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor