Some properties of human small heat shock protein Hsp22 (H11 or HspB8).
Untagged recombinant human small heat shock protein with apparent molecular mass 22 kDa (Hsp22) was obtained in homogeneous state. Size exclusion chromatography and chemical crosslinking with dimethylsuberimidate indicate that Hsp22 forms stable dimers. Being highly susceptible to oxidation Hsp22 forms disulfide crosslinked dimers and poorly soluble high molecular mass oligomers. According to CD spectroscopy oxidation of Hsp22 results in disturbing of both secondary and tertiary structure. Hsp22 possesses a negligibly low autophosphorylation activity and under the conditions used is unable to phosphorylate casein or histone. Hsp22 effectively prevents heat-induced aggregation of yeast alcohol dehydrogenase and bovine liver rhodanese with chaperone activity comparable to that of recombinant human small heat shock protein with apparent molecular mass 20 kDa (Hsp20).
Department of Biochemistry, School of Biology, Moscow State University, Moscow 119992, Russia.
Maria V
MV
Kim
Alim S
AS
Seit-Nebi
Steven B
SB
Marston
Nikolai B
NB
Gusev
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D047388:Casein Kinases; D002850:Chromatography, Gel; D002942:Circular Dichroism; D016384:Consensus Sequence; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D050886:HSP20 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000201:enzymology; D008623:Mercaptoethanol / Q000737:chemistry; D018832:Molecular Chaperones / Q000378:metabolism; D008969:Molecular Sequence Data; D009124:Muscle Proteins; D010084:Oxidation-Reduction; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D020836:Protein Structure, Quaternary; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D050886:HSP20 Heat-Shock Proteins; C413214:HSPB8 protein, human; D006360:Heat-Shock Proteins; C490753:HspB8 protein, rat; D018832:Molecular Chaperones; D009124:Muscle Proteins; D011994:Recombinant Proteins; C087458:Hspb6 protein, rat; D008623:Mercaptoethanol; D011494:Protein Kinases; D047388:Casein Kinases; D017346:Protein Serine-Threonine Kinases; D017868:Cyclic AMP-Dependent Protein Kinases