[IL-1alpha, IL-1beta and TNF-alpha mRNA expression in experimental periapical lesions in the rat].
OBJECTIVE
IL-1alpha, IL-1beta and TNF-alpha mRNA expression were analyzed in rat experimental periapical lesions to understand the relationship between the aforementioned cytokines and periapical lesion.


METHODS
The molar tooth pulps of 16 SD rats were surgically exposed and left open to permit infection from the oral environment. Total cell RNA was isolated from periapical tissue obtained on days 3, 7, 14 and 28 after exposure. mRNA for IL-1alpha, IL-1beta and TNF-alpha was amplified by reverse transcription polymerase chain reaction(RT-PCR), and levels were estimated by comparison to the parallel amplification of the housekeeping gene beta-actin.


RESULTS
IL-1alpha,IL-1beta and TNF-alpha mRNA were all expressed beginning on day 7, peaked on day 14, and declined somewhat on day 28, but IL-1beta mRNA was expressed at much lower levels with the same kinetics (P<0.01).


CONCLUSIONS
IL-1alphaand TNF-alpha may be primarily cytokines in rat periapical lesion model.
Department of Oral Medicine, School of Stomatology, Shanghai Second Medical University, Shanghai 200011, China.
L X
LX
Mei
Z
Z
Liu
B
B
Zhang