Targeting duplex DNA with DNA-PNA chimeras? Physico-chemical characterization of a triplex DNA-PNA/DNA/DNA.
Targeting double-stranded DNA with homopyrimidine PNAs results in strand displacement complexes PNA/DNA/PNA rather than PNA/DNA/DNA triplex structures. Not much is known about the binding properties of DNA-PNA chimeras. A 16-mer 5'-DNA-3'-p-(N)PNA(C) has been investigated for its ability to hybridize a complementary duplex DNA by DSC, CD, and molecular modeling studies. The obtained results showed the formation of a triplex structure having similar, if not slightly higher, stability compared to the same all-DNA complex.
Dipartimento di Chimica, Università "Federico II" di Napoli, Via Cintia 4, 80126-Napoli, Italy.
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Petraccone
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Erra
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Messere
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Montesarchio
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Piccialli
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De Napoli
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Barone
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Giancola
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002678:Chimera; D002942:Circular Dichroism; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D011742:Pyrimidine Nucleotides / Q000737:chemistry; D013696:Temperature; D013816:Thermodynamics
D009838:Oligodeoxyribonucleotides; D020135:Peptide Nucleic Acids; D011742:Pyrimidine Nucleotides; C051802:triplex DNA; D004247:DNA