Novel FLT3 point mutations within exon 14 found in patients with acute myeloid leukaemia.
Internal tandem duplications in FLT3 are the most common mutation in acute myeloid leukaemia (AML), with agarose gel electrophoresis of polymerase chain reaction products (PCR/agarose) being the screening method of choice for these mutations. As PCR/agarose screening does not detect small mutations, single-stranded conformational polymorphism analyses (PCR/SSCP) were used in an attempt to identify previously unrecognized point mutations in FLT3 exons 14 and 15 of 140 AML patients, using newly designed primers that anneal within intron sequences. Novel missense point mutations were found in exon 14, suggesting additional investigations should be performed in AML and other haematopoietic malignancies, using this sensitive technique.
Clinical Research Division, Fred Hutchinson Cancer Research Center, and Division of Oncology, University of Washington, Seattle, WA 98109, USA. dstirewa@fhcrc.org
Derek L
DL
Stirewalt
Soheil
S
Meshinchi
Steven J
SJ
Kussick
Kayla M
KM
Sheets
Era
E
Pogosova-Agadjanyan
Cheryl L
CL
Willman
Jerald P
JP
Radich
D000208:Acute Disease; D005091:Exons / Q000235:genetics; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics*; D020125:Mutation, Missense; D009363:Neoplasm Proteins / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D051941:fms-Like Tyrosine Kinase 3
D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins; C497970:FLT3 protein, human; D020794:Receptor Protein-Tyrosine Kinases; D051941:fms-Like Tyrosine Kinase 3