[Colonization of silicate bacterium strain NBT in wheat roots].
The strain NBT of silicate bacterium was labelled with streptomycin, and a stable streptomycin resistance strain NBT was obtained. Its colonization dynamics and affecting factors in wheat rhizosphere were studied in agar plates and greenhouse pots were studied by counting the method with selective medium. The results of pot culture experiment showed that strain NBT could successfully colonize in the rhizosphere of wheat. In pot cultures of sterile soil, the highest colonization level (3.4 x 10(7) cfu.g-1 root soil) was reached on 9th day after seeds sown; at 54th day, the population of strain NBT tended to stable, and decreased to 1.4 x 10(4) cfu.g-1 root soil. In pot cultures of unsterile soil, the highest colonization level (3.8 x 10(7) cfu.g-1 root soil) was reached at 9th day, and the population of strain NBT tended to a stationary state at 60th day, with the numbers being 1.4 x 10(4) cfu.g-1 root soil. Some biological and abiotic factors could greatly influence the colonization of the beneficial microorganism.
Sheng
Xiafang
X
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China. xfsheng604@sohu.com
D018517:Plant Roots / Q000382:microbiology*; D012230:Rhizobiaceae / Q000187:drug effects / Q000254:growth & development*; D013307:Streptomycin / Q000494:pharmacology; D014908:Triticum / Q000382:microbiology*
D013307:Streptomycin