Gene expression profiling of mouse condylar cartilage during mastication by means of laser microdissection and cDNA array.
Little is known about the mechanisms of mandibular condylar growth. In this study, gene expression in the mandibular condylar cartilage of young post-natal mice was monitored by means of a cDNA microarray, real-time PCR, and laser microdissection before and after the initiation of mastication (newborn, 7 days, 21 days, initiation of mastication, and 35 days). Insulin-like growth factor-1 (IGF-I), transforming-growth-factor-beta-2 (TGFbeta2), and aggrecan mRNAs were clearly expressed at 21 days, while the expression of osteopontin mRNAs was most clear at 35 days. Parathyroid-hormone-related protein (PTHrP), Indian-hedgehog (Ihh), and insulin-like growth factor-2 (IGF-2) mRNAs were clearly expressed during lactation (newborn and 7 days). Heat-shock-protein 84 (HSP-84) and heat-shock-protein 86 (HSP-86) were clearly expressed at 35 days. These results revealed that gene expression changed during mandibular condylar cartilage growth, and that, interestingly, these changes coincided with the initiation of mastication.
Department of Orthodontics and Department of Oral Pathology, School of Dentistry, Showa University, 2-1-2 Kitasenzoku, Ohta-ku, Tokyo 145-8515, Japan. junwata2000@ybb.ne.jp
J
J
Watahiki
T
T
Yamaguchi
T
T
Irie
H
H
Nakano
K
K
Maki
T
T
Tachikawa
D053674:Aggrecans; D000818:Animals; D002356:Cartilage / Q000254:growth & development*; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis; D016326:Extracellular Matrix Proteins; D020869:Gene Expression Profiling; D006132:Growth Plate / Q000254:growth & development*; D006360:Heat-Shock Proteins / Q000032:analysis; D053823:Hedgehog Proteins; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D007335:Insulin-Like Growth Factor II / Q000032:analysis; D053685:Laser Therapy; D037181:Lectins, C-Type; D008297:Male; D008335:Mandibular Condyle / Q000254:growth & development*; D008409:Mastication / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D042282:Microdissection; D020411:Oligonucleotide Array Sequence Analysis; D053495:Osteopontin; D044162:Parathyroid Hormone-Related Protein / Q000032:analysis; D010750:Phosphoproteins / Q000032:analysis; D011509:Proteoglycans / Q000032:analysis; D012795:Sialoglycoproteins / Q000032:analysis; D015534:Trans-Activators / Q000032:analysis; D016212:Transforming Growth Factor beta / Q000032:analysis; D053781:Transforming Growth Factor beta2
C508320:Acan protein, mouse; D053674:Aggrecans; D011508:Chondroitin Sulfate Proteoglycans; D016326:Extracellular Matrix Proteins; D006360:Heat-Shock Proteins; D053823:Hedgehog Proteins; D037181:Lectins, C-Type; D044162:Parathyroid Hormone-Related Protein; D010750:Phosphoproteins; D011509:Proteoglycans; D012795:Sialoglycoproteins; C506896:Spp1 protein, mouse; D015534:Trans-Activators; D016212:Transforming Growth Factor beta; D053781:Transforming Growth Factor beta2; D053495:Osteopontin; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II