Increased expression of two phospholipase D isoforms during experimentally induced hippocampal mossy fiber outgrowth.
Mammalian phospholipase D (PLD), a multifunctional signaling enzyme, has been reported to facilitate neurite outgrowth in cultured neurons. However, two mammalian isoforms have been found, PLD1 and PLD2, and it has not been determined which isoform is involved, or whether this in vitro phenomenon is relevant to neurite extension in vivo. Using confocal microscopy, we demonstrate that the PLDs are expressed by different cell types in the mouse brain: PLD1 by neurons, and PLD2 by astrocytes. Moreover, using a model of experimentally induced hippocampal mossy fiber sprouting, both isoforms were observed to increase dramatically in expression level along tracts of mossy fiber spouting, supporting the proposal that PLD plays a role in this process. Given that the two isoforms undertake unique molecular functions in cultured cells, our findings suggest that in vivo PLD1 and PLD2 may modulate neuronal plasticity via different pathways and cell types.
Zhang
Yan
Y
Department of Pharmacological Sciences, University Medical Center at Stony Brook, Stony Brook, New York 11794-8651, USA.
Huang
Ping
P
Du
Guangwei
G
Kanaho
Yasunori
Y
Frohman
Michael A
MA
Tsirka
Stella E
SE
D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D007608:Kainic Acid / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019599:Mossy Fibers, Hippocampal / Q000187:drug effects / Q000201:enzymology*; D010739:Phospholipase D / Q000096:biosynthesis* / Q000235:genetics
D007527:Isoenzymes; C105606:phospholipase D2; D010739:Phospholipase D; C106801:phospholipase D1; D007608:Kainic Acid