Integration of high-resolution array comparative genomic hybridization analysis of chromosome 16q with expression array data refines common regions of loss at 16q23-qter and identifies underlying candidate tumor suppressor genes in prostate cancer.
We have constructed a high-resolution genomic microarray of human chromosome 16q, and used it for comparative genomic hybridization analysis of 16 prostate tumors. We demarcated 10 regions of genomic loss between 16q23.1 and 16qter that occurred in five or more samples. Mining expression array data from four independent studies allowed us to identify 11 genes that were frequently underexpressed in prostate cancer and that co-localized with a region of genomic loss. Quantitative expression analyses of these genes in matched tumor and benign tissue from 13 patients showed that six of these 11 (WWOX, WFDC1, MAF, FOXF1, MVD and the predicted novel transcript Q9H0B8 (NM_031476)) had significant and consistent downregulation in the tumors relative to normal prostate tissue expression making them candidate tumor suppressor genes.
Watson
J E Vivienne
JE
Collins Lab, UCSF Comprehensive Cancer Center, University of California, 2340 Sutter Street, San Francisco, USA. vwatson@cc.ucsf.edu
Doggett
Norman A
NA
Albertson
Donna G
DG
Andaya
Armann
A
Chinnaiyan
Arul
A
van Dekken
Herman
H
Ginzinger
David
D
Haqq
Christopher
C
James
Karen
K
Kamkar
Sherwin
S
Kowbel
David
D
Pinkel
Daniel
D
Schmitt
Lars
L
Simko
Jeffry P
JP
Volik
Stanislav
S
Weinberg
Vivian K
VK
Paris
Pamela L
PL
Collins
Colin
C
D002885:Chromosomes, Human, Pair 16; D016147:Genes, Tumor Suppressor; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D011471:Prostatic Neoplasms / Q000235:genetics*