Agrobacterium tumefaciens-mediated transformation of Beauveria bassiana using an herbicide resistance gene as a selection marker.
Beauveria bassiana has been investigated for use in the biological control of several insects in agricultural practice. To understand the molecular basis of virulence and host specificity and to improve the entomopathogenicity of B. bassiana, we have developed a simple, highly efficient and reliable Agrobacterium-mediated transformation method for B. bassiana using a phosphinothricin acetyltransferase (bar) gene as a selectable marker. Most transformants contained single copies of T-DNA and the T-DNA inserts were stably inherited after five generations. With this highly efficient transformation method for B. bassiana, we also obtained two putative T-DNA-tagged mutants that may have altered growth habits or virulence. Thus, the described protocol could provide a useful tool to manipulate the genetic make-up and to tag genes that may be important for virulence or growth and development of B. bassiana.
Fang
Weiguo
W
Biotechnology Research Center, Southwest Agricultural University, Beibei, Chongqing 400716, PR China.
Zhang
Yongjun
Y
Yang
Xingyong
X
Zheng
Xuelian
X
Duan
Hui
H
Li
Yi
Y
Pei
Yan
Y
D016960:Agrobacterium tumefaciens / Q000502:physiology*; D000818:Animals; D032661:Cordyceps / Q000502:physiology*; D004269:DNA, Bacterial / Q000235:genetics; D010572:Pest Control, Biological / Q000379:methods*; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic
D004269:DNA, Bacterial; C036483:T-DNA