The effects of a caries-removing gel (Carisolv) on FM3A cell-line in vitro.
OBJECTIVE
The aim of this study was to examine the cytotoxic effects of Carisolv on mouse mammary carcinoma cell line (FM3A) at different application times.


METHODS
The FM3A cell line obtained from the European Collection of Animal Cell Cultures was used in the cell culture assays. Exponentially growing cells were seeded in 5x10(5)cells/well in 5 ml of RPMI 1640 medium supplemented with 10(%) fetal calf serum and antibiotics in each well of a six-well plate. Carisolv gel was applied onto the cell culture medium for 1, 5 and 20 min and incubated for 24 h at 37 degrees C in a humidified atmosphere of 5(%) carbon dioxide (CO(2)). After 24 h incubation, the cells were collected by trypsinisation and counted with a hemocytometer. The cytotoxicity of the Carisolv was determined by evaluation of cell growth and viability in comparison to untreated controls (cell growth=100%). For cell viability, the trypane blue exclusion assay was used. Dunnett's t-test was used for statistical analysis.


RESULTS
Cell growth was significantly reduced after 20 min application of Carisolv in comparison to the control (p < 0.001) and 1 min treatment groups (p < 0.05) No significant differences were found in cell viability between the study groups.


CONCLUSIONS
It can be concluded that prolonged application of Carisolv did not affect cell viability, but had a reducing effect on cell growth in the FM3A cell line.
Department of Pedodontics, Faculty of Dentistry, University of Istanbul, Capa, 34390 Istanbul, Turkey. alicansepet@yahoo.com
Elif
E
Sepet
Ayhan
A
Bilir
Oya
O
Akçin
Zeynep
Z
Aytepe
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003737:Dental Cavity Preparation / Q000379:methods*; D018698:Glutamic Acid / Q000633:toxicity*; D007930:Leucine / Q000633:toxicity*; D008239:Lysine / Q000633:toxicity*; D051379:Mice
C119934:Carisolv; D018698:Glutamic Acid; D007930:Leucine; D008239:Lysine