Studies on the influence of vitamin D3 metabolites on apoptosis induction in human neoplastic cells.
The vitamin D is involved in essential cell regulatory processes such as proliferation and differentiation in a number of different cell types including cancer cells. Adriamycin is one of the most effective agents in the treatment of many types of solid tumours and leukemias. The common features in the biological activity, expressed by vitamin D family members and adriamycin such as: apoptosis induction, influence on antioxidant enzymes activity etc., created the suggestion of synergistic effects of these compounds combination. In the earlier studies the antiproliferative activity of vitamin D3 metabolites was shown in ME18 cells, but without any correlation with sensitivity of the cells to adriamycin. In the current work the possible role of 25-hydroxycholecalciferol (calcidiol) and 1,25-dihydroxycholecalciferol (calcitriol) as the apoptotic inducers was studied in human melanoma cells. As was shown in these experiments, vitamin D3 metabolites did not stimulate apoptotic events in the cells studied and did not influence apoptosis induction in the cells treated with adriamycin.
Department of Biochemistry and Biopharmaceuticals, National Institute of Public Health, 30/34 Chelmska Str., 00-725 Warsaw, Poland.
Beata M
BM
Gruber
Elzbieta L
EL
Anuszewska
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002112:Calcifediol / Q000494:pharmacology*; D002117:Calcitriol / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D002762:Cholecalciferol / Q000378:metabolism; D004317:Doxorubicin / Q000494:pharmacology; D004347:Drug Interactions; D005434:Flow Cytometry; D006801:Humans; D008545:Melanoma
D000970:Antineoplastic Agents; D002762:Cholecalciferol; D004317:Doxorubicin; D002117:Calcitriol; D002112:Calcifediol