Colorimetric detection of protein microarrays based on nanogold probe coupled with silver enhancement.
This work presents a method for analyzing protein microarrays using a colorimetric nanogold probe coupled with silver enhancement (gold-silver detection). In this method, the gold nanoparticles were introduced to the microarray by the specific binding of the gold-conjugated antibodies or streptavidins and then coupled with silver enhancement to produce black image of microarray spots, which can be easily detected with a commercial CCD camera. The method showed high detection sensitivity (1 pg of IgG immobilized on slides or 2.75 ng/ml IgG in solution) and a good linear correlation between the signal intensity and the logarithm of the sample concentration. The examination of this method in analyzing a demonstrational ToRCH antigen microarray developed in our lab showed an identical result as in the fluorescent method. These results suggest the colorimetric gold-silver detection method has potential applications in proteomics research and clinical diagnosis.
Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China.
Ru-Qiang
RQ
Liang
Cui-Yan
CY
Tan
Kang-Cheng
KC
Ruan
D000818:Animals; D003124:Colorimetry / Q000379:methods*; D006046:Gold; D006801:Humans; D040081:Protein Array Analysis; D012680:Sensitivity and Specificity; D012834:Silver
D012834:Silver; D006046:Gold