Pd(II) and Pt(II) complexes with aromatic diamines: study of their interaction with DNA.
Pd(II) and Pt(II) new complexes with simple aromatic diamines were synthesised and characterised with the aim of studying their possible antitumour activity. The aromatic diamines chosen were 2,3-diaminotoluene (2,3 dat), 3,4-diaminotoluene (3,4 dat), 4,5-diaminoxylene (4,5 dax) and 2,3-diaminophenol (2,3 dap). The complexes, of formulae cis-[MCl(2)(diamine)], were characterised by elemental analysis, conductivity measurements, 1H, 13C(1H) and 195Pt NMR spectroscopy. The X-ray crystal structure was also resolved for the palladium complexes with 2,3-diaminotoluene and 4,5-diaminoxylene. The DNA adduct formation of the eight new complexes synthesised was followed by circular dichroism and electrophoretic mobility. Atomic force microscopy images of the modifications caused by the complexes on plasmid DNA pBR322 were also obtained. Values of IC50 were also calculated for the four platinum complexes against the cisplatin resistant tumour cell line A2780cisR.
Departament de Química Inorgànica, Universitat de Barcelona, Diagonal 647, E-08028 Barcelona, Spain.
M
M
Pérez-Cabré
G
G
Cervantes
V
V
Moreno
M J
MJ
Prieto
J M
JM
Pérez
M
M
Font-Bardia
X
X
Solans
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D002417:Cattle; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004247:DNA / Q000378:metabolism*; D003959:Diamines / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D024202:Electrophoretic Mobility Shift Assay; D006801:Humans; D006841:Hydrocarbons, Aromatic / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009942:Organometallic Compounds / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D009944:Organoplatinum Compounds / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D010165:Palladium / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology
D000970:Antineoplastic Agents; D003959:Diamines; D006841:Hydrocarbons, Aromatic; D009942:Organometallic Compounds; D009944:Organoplatinum Compounds; D010165:Palladium; D004247:DNA; C427203:calf thymus DNA; D002945:Cisplatin