Real-time multiplex PCR assay for detection of Brucella spp., B. abortus, and B. melitensis.
The identification of Brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. Here, we describe a real-time PCR assay for confirmation of presumptive Brucella isolates. The assay was designed in a multiplex format that will allow the rapid identification of Brucella spp., B. abortus, and B. melitensis in a single test.
Microbial Diseases Laboratory, Division of Communicable Disease Control, California Department of Health Services, Richmond, California 94804, USA. wprobert@dhs.ca.gov
William S
WS
Probert
Kimmi N
KN
Schrader
Nhi Y
NY
Khuong
Susan L
SL
Bystrom
Margot H
MH
Graves
D001483:Base Sequence; D002002:Brucella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002003:Brucella abortus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017347:Brucella melitensis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D003199:Computer Systems; D017931:DNA Primers; D016133:Polymerase Chain Reaction / Q000379:methods*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D017931:DNA Primers; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S