[Clonal expansion of TCR Vbeta subfamily T cells induced by bcr3-abl2 peptide].
OBJECTIVE
To investigate the clonal expansion of T cell receptor (TCR) Vbeta subfamily T cells from cord blood induced by bcr3-abl2 peptide in vitro.


METHODS
T cells from 3 units of cord blood were amplified by anti-CD(3) monoclonal antibody (McAb) and IL-2 with or without synthetic b3a2 peptide. T cell specific cytotoxicity was analyzed by lactate dehydrogenase (LDH) assay, TCR Vbeta subfamilies by using reverse transcriptase-polymerase chain reaction (RT-PCR) and genescan technique.


RESULTS
bcr3-abl2 peptide specific cytotoxicity T cells were successfully induced from the 3 units of cord blood by synthetic b3a2 peptide. Compared with that in CD(3) McAb induced cells, distribution pattern of TCR Vbeta repertoire was different in T cells induced with b3a2 peptide. Oligoclonal and oligoclonal tendency TCR Vbeta subfamily T cells could be identified in cord blood T cells induced by b3a2 peptide in 1 or 2 weeks, whereas those induced by anti-CD(3) McAb and IL-2 were mostly polyclonal.


CONCLUSIONS
The cytotoxicity T cells with anti-CML specificity could be induced by b3a2 peptide. The specific anti-CML cytotoxicity may be derived from the clonal expansion TCR Vbeta subfamily T cells.
Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China.
Yu-ping
YP
Zhang
Yang-qiu
YQ
Li
Li-jian
LJ
Yang
Shao-hua
SH
Chen
Xue-li
XL
Zhang
Zhen
Z
Wang
Xiu-li
XL
Wu
Geng-xin
GX
Luo
D000911:Antibodies, Monoclonal / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D016044:Fusion Proteins, bcr-abl / Q000494:pharmacology*; D019674:Genes, T-Cell Receptor beta; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D007376:Interleukin-2; D016044:Fusion Proteins, bcr-abl