A simple solid phase mass tagging approach for quantitative proteomics.
New mass-tagging reagents for quantitative proteomics measurements have been designed using solid phase peptide synthesis technology. The solid phase mass tags have been used to accurately measure the relative amounts of cysteine-containing peptides in model peptide mixtures as well as in mixtures of tryptic digests in the femtomol range. Measurements were made using both matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and online reversed-phase capillary liquid chromatography coupled through a nanoelectrospray interface to an ion trap mass spectrometer (capillary LC/ESI-MS). Results of mass-tagging experiments obtained from these two mass spectrometry techniques and their relative advantages and disadvantages for identification and quantitation of mass tagged peptides are compared. These reagents provide a simple, rapid and cost-effective alternative to currently available mass tagging technologies.
Shi
Yang
Y
Department of Chemical Engineering, Yale University, New Haven, Connecticut 06520-8286, USA.
Xiang
Rong
R
Crawford
Janet K
JK
Colangelo
Christopher M
CM
Horváth
Csaba
C
Wilkins
James A
JA
D002851:Chromatography, High Pressure Liquid; D015335:Molecular Probes / Q000138:chemical synthesis*; D010455:Peptides / Q000138:chemical synthesis; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D015335:Molecular Probes; D010455:Peptides; D011506:Proteins