Laser capture microdissection and mRNA characterization of mouse airway epithelium: methodological considerations.
The use of laser capture microdissection (LCM) to obtain epithelial cells lining the distal airways for gene profiling is described. In the mouse, the distal airways are particularly attractive for LCM as there is very high percentage of a single cell type, Clara cells, lining these airways. It is shown that the RNA from distal airway epithelial cells harvested by LCM is well preserved and that with linear amplification sufficient cRNA for microarray analysis can be attained from small numbers of cells.
First Department of Medicine, Hokkaido University School of Medicine, Sapporo, Japan. bytomoko@med.hokudai.ac.jp
Tomoko
T
Betsuyaku
Robert M
RM
Senior
D000818:Animals; D001980:Bronchi / Q000166:cytology / Q000378:metabolism; D002469:Cell Separation / Q000379:methods; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D020869:Gene Expression Profiling; D007834:Lasers; D008168:Lung / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D042282:Microdissection / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D020411:Oligonucleotide Array Sequence Analysis; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*
D012333:RNA, Messenger