Gender differences in lipoprotein lipase activity after acute exercise.
OBJECTIVE
To determine whether gender differences exist in lipoprotein lipase (LPL) activity in response to exercise and/or insulin. Exercise and insulin are known modulators of LPL activity in men, but this is less clear in women. LPL activity may predict propensity for obesity; therefore, understanding its modulators is of considerable importance.


METHODS
Gender differences in skeletal muscle and adipose tissue LPL activity were determined after a single bout of exercise followed by a hyperinsulinemic/euglycemic clamp and compared with an identical rest day in healthy lean men (n = 10) and women (n = 10). Muscle and adipose tissue biopsies were obtained pre- (post-exercise vs. rest) and post-clamp.


RESULTS
Basal levels of muscle and adipose tissue LPL activity were not different between men and women. There was, however, a significant gender by day interaction for muscle LPL activity (p = 0.023) and adipose tissue LPL activity (p = 0.013). In muscle, this was because of a significant increase in LPL activity on the exercise vs. rest day in men (p < 0.001) but not women. Adipose tissue LPL activity also increased significantly in men on the exercise day relative to rest day (p = 0.04) but decreased in women (p = 0.10). The hyperinulinemic/euglycemic clamp had no independent effect on tissue LPL activity, in either gender, after rest or exercise.


CONCLUSIONS
In the 3 to 4 hours after exercise, muscle and adipose tissue LPL activity increased significantly in men, whereas LPL activity remained unchanged in women.
Department of Medicine, Division of Endocrinology, Metabolism and Diabetes, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA. leigh.perreault@uchsc.edu
Leigh
L
Perreault
Jennifer M
JM
Lavely
John M
JM
Kittelson
Tracy J
TJ
Horton
D000273:Adipose Tissue / Q000201:enzymology*; D000328:Adult; D015444:Exercise / Q000502:physiology*; D005260:Female; D015309:Glucose Clamp Technique; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000201:enzymology*; D010101:Oxygen Consumption; D012727:Sex Characteristics
D007328:Insulin; D008071:Lipoprotein Lipase