Display of heterologous antigens on the Bacillus subtilis spore coat using CotC as a fusion partner.
We report the use of CotC, a major component of the Bacillus subtilis spore coat, as a fusion partner for the expression of two heterologous antigens on the spore coat. Recombinant spores expressing tetanus toxin fragment C (TTFC) of Clostridium tetani or the B subunit of the heat-labile toxin of Escherichia coli (LTB) were used for oral dosing and shown to generate specific systemic and mucosal immune responses in a murine model. This report, expanding the previously described expression of TTFC on the spore surface by fusion to CotB [J Bacteriol 183 (2001) 6294] and its use for oral vaccination [Infect Immun 71 (2003) 2810] shows that different antigens can be successfully presented on the spore coat and supports the use of the spore as an efficient vehicle for mucosal immunisation.
Dipartimento di Fisiologia Generale ed Ambientale, Sezione di Microbiologia, Università Federico II, Napoli, Italy.
Emilia M F
EM
Mauriello
Le H
le H
Duc
Rachele
R
Isticato
Giuseppina
G
Cangiano
Huynh A
HA
Hong
Maurilio
M
De Felice
Ezio
E
Ricca
Simon M
SM
Cutting
D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D001412:Bacillus subtilis / Q000737:chemistry / Q000276:immunology*; D001427:Bacterial Toxins / Q000276:immunology; D015153:Blotting, Western; D002876:Chromosomes, Bacterial / Q000276:immunology; D003512:Cyclohexanones / Q000737:chemistry / Q000276:immunology*; D004768:Enterotoxins / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D029968:Escherichia coli Proteins; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics; D018928:Immunity, Mucosal / Q000276:immunology; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology; D013171:Spores, Bacterial / Q000737:chemistry / Q000276:immunology*; D013742:Tetanus / Q000276:immunology; D013744:Tetanus Toxin / Q000276:immunology; D014169:Transformation, Bacterial; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D003512:Cyclohexanones; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D013744:Tetanus Toxin; D014614:Vaccines, Synthetic; C040635:tetanus toxin fragment C; C011202:2-crotonyloxymethyl-(4R,5R,6R)-4,5,6-trihydroxycyclohex- 2-enone; C027538:heat-labile enterotoxin, E coli