Amine donor protein substrates for transglutaminase activity in Caenorhabditis elegans.
Transglutaminase dependent cross-linking of proteins has been implicated in a wide range of biological phenomena occurring in both extracellular and intracellular compartments. Clarification of the physiological role of transglutaminases requires identification of substrate molecules. Here we report the detection, purification, and identification by mass spectrometry of proteins, the glutamate dehydrogenase, a protein disulfide isomerase, and aldehyde dehydrogenase as amine donor substrates for the transglutaminase activity of the nematode Caenorhabditis elegans utilizing a novel biotinylated oligoglutamine peptide as a substrate. We also purified and identified streptavidin-binding proteins of the worm.
Signalling and Apoptosis Research Group of the Hungarian Academy of Sciences, University of Debrecen, Debrecen, Hungary. madi@indi.biochem.dote.hu
András
A
Mádi
Raimund
R
Hoffrogge
Bernadett
B
Blaskó
Michael O
MO
Glocker
László
L
Fésüs
D000199:Actins / Q000737:chemistry / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D000588:Amines / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D019921:Biotinylation; D017173:Caenorhabditis elegans / Q000201:enzymology* / Q000254:growth & development; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019735:Carbon-Carbon Ligases / Q000737:chemistry / Q000378:metabolism; D005969:Glutamate Dehydrogenase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005973:Glutamine / Q000031:analogs & derivatives / Q000378:metabolism; D007814:Larva / Q000201:enzymology; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism; D010446:Peptide Fragments / Q000235:genetics; D019704:Protein Disulfide-Isomerases / Q000737:chemistry / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D019809:Streptavidin / Q000378:metabolism; D013379:Substrate Specificity; D011503:Transglutaminases / Q000737:chemistry / Q000378:metabolism*
D000199:Actins; D000588:Amines; D029742:Caenorhabditis elegans Proteins; D009842:Oligopeptides; D010446:Peptide Fragments; D005973:Glutamine; D019809:Streptavidin; D000444:Aldehyde Dehydrogenase; D005969:Glutamate Dehydrogenase; D011503:Transglutaminases; D019704:Protein Disulfide-Isomerases; D019735:Carbon-Carbon Ligases