Isolation and characterization of wild type yellow fever virus in cases temporally associated with 17DD vaccination during an outbreak of yellow fever in Brazil.
A mass vaccination was carried out in the state of Minas Gerais, Southeast region of Brazil, to control an outbreak of sylvatic yellow fever in 2001. During the outbreak the surveillance system identified two fatal cases temporally associated with YF vaccination. Virus recovered from blood and postmortem samples of both cases was identified as yellow fever virus. Partial nucleotide sequence of parts of prM/E and the non-structural (NS) 5 genes and 3' non-coding region (3' NCR) was employed to characterize the origin of yellow fever virus (YFV) involved in both cases. Wild-type YFV was identified as the etiologic agent responsible for the disease.
Departamento de Virologia, Laboratório de Flavivírus, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brazil 4365, 21045-900 Rio de Janeiro, RJ, Brazil. ambispo@ioc.fiocruz.br
Ana Maria Bispo de
AM
Filippis
Rita Maria Ribeiro
RM
Nogueira
Alfredo Verlangieri
AV
Jabor
Hermann G
HG
Schatzmayr
Janice Coelho
JC
Oliveira
Sonia C Machado
SC
Dinis
Ricardo
R
Galler
D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D001938:Brazil; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis / Q000096:biosynthesis; D008297:Male; D032541:Mass Vaccination / Q000009:adverse effects*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology; D015004:Yellow Fever / Q000175:diagnosis / Q000635:transmission* / Q000821:virology*; D015005:Yellow fever virus / Q000235:genetics / Q000276:immunology*
D007075:Immunoglobulin M; D012367:RNA, Viral; D014759:Viral Envelope Proteins