Construction and immunogenic characterization of a fusion anti-caries DNA vaccine against PAc and glucosyltransferase I of Streptococcus mutans.
Glucosyltransferases (GTFs) and A cell-surface protein (PAc) are two important virulence factors of the cariogenic organism Streptococcus mutans. They may mediate sucrose-independent or sucrose-dependent attachment of Streptococcus mutans to tooth surfaces, respectively. Thus, inhibiting both virulence factors is predicted to provide better protection against caries than inhibiting a single factor. To develop a highly efficient vaccine against caries, we constructed a fusion DNA vaccine, pGLUA-P, by cloning the GLU region of GTF into a DNA vaccine, pCIA-P, which encodes two highly conservative regions of PAc. In this report, we provide evidence that fewer caries lesions were observed in rats following subcutaneous injection of pGLUA-P, compared with pCIA-P, near the submandibular gland. Our findings suggest that a multigenic DNA vaccine may be more caries-preventive than a single-gene DNA vaccine.
Key Lab. for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, PR China.
J H
JH
Guo
R
R
Jia
M W
MW
Fan
Z
Z
Bian
Z
Z
Chen
B
B
Peng
D018829:Adhesins, Bacterial / Q000276:immunology*; D000704:Analysis of Variance; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D001426:Bacterial Proteins / Q000276:immunology*; D003731:Dental Caries / Q000517:prevention & control*; D000939:Epitopes; D005964:Glucosyltransferases / Q000276:immunology*; D007114:Immunization; D007279:Injections, Subcutaneous; D008297:Male; D008562:Membrane Glycoproteins; D011506:Proteins / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D022541:Streptococcal Vaccines / Q000138:chemical synthesis*; D013295:Streptococcus mutans / Q000276:immunology*; D019444:Vaccines, DNA / Q000138:chemical synthesis*; D014774:Virulence
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D000939:Epitopes; D008562:Membrane Glycoproteins; D011506:Proteins; C055764:S-layer proteins; D022541:Streptococcal Vaccines; D019444:Vaccines, DNA; C067118:GTF-I protein, Streptococcus; D005964:Glucosyltransferases; C489364:glucosyltransferase I