In vitro expression and pharmacology of the 5-HT7-like receptor present in the mosquito Aedes aegypti tracheolar cells and hindgut-associated nerves.
We have previously reported the cloning of a 5-hydroxytryptamine receptor (Aedes 5-HT7-like receptor) from adult Aedes aegypti. For functional expression of the Aedes 5-HT7-like receptor, CHO-K1 cells were stably transfected with a receptor expression construct, pC5-HT7. The Aedes 5-HT7-like receptor positively coupled to Gs protein, increasing intracellular cAMP in response to 5-HT; adenylyl cyclase activity was induced in a concentration-dependent, saturable manner. Only 5-HT, and not octopamine, dopamine or tyramine, caused the induction of cAMP. At 10 nM 5-HT a weak synergism was observed between octopamine and 5-HT. Other known agonists of the mammalian 5-HT7 receptor were tested. Their order of potency was: 5-HT >> 5-CT = 8-OH-DPAT >> pimozide. This is the first report on the functional expression of a mosquito neurohormone receptor.
Lee
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DW
Department of Entomology, Texas A & M University, College Station, TX 77843-2475, USA.
Pietrantonio
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PV
D000262:Adenylyl Cyclases / Q000378:metabolism; D000330:Aedes / Q000235:genetics*; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D000242:Cyclic AMP / Q000096:biosynthesis; D017931:DNA Primers; D004298:Dopamine; D015870:Gene Expression; D066298:In Vitro Techniques; D009655:Octopamine; D011985:Receptors, Serotonin / Q000235:genetics* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012701:Serotonin / Q000378:metabolism; D014162:Transfection; D014439:Tyramine
D017931:DNA Primers; D011985:Receptors, Serotonin; C104425:serotonin 7 receptor; D009655:Octopamine; D012701:Serotonin; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D004298:Dopamine; D014439:Tyramine