The Q-Trap mass spectrometer, a novel tool in the study of protein glycosylation.
The use of the recently introduced Q-Trap mass spectrometer in the study of protein glycosylation is described. The combined ion trap and triple quadrupole scan functions make it a powerful system in both oligosaccharide and glycopeptide analysis. Several oligosaccharides, both linear and branched, were analyzed to obtain information on sequence, linkage, and branching. Quadrupole like MS/MS spectra with ion trap sensitivity but without the typical ion trap low mass cut-off were obtained. To determine the origin of fragments and to reveal the existence of new ions, the MS(3) capabilities of the system proved to be useful. Glycopeptides were selectively detected in peptide mixtures using the triple quadrupole precursor ion scan function, either in off-line experiments or during LC/MS using information dependent acquisition (IDA).
Laboratory of Protein Biochemistry and Protein Engineering, Ghent University, Ghent, Belgium.
K
K
Sandra
B
B
Devreese
J
J
Van Beeumen
I
I
Stals
M
M
Claeyssens
D000818:Animals; D002417:Cattle; D005936:Glucans / Q000737:chemistry; D006020:Glycopeptides / Q000737:chemistry; D006031:Glycosylation; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods*; D015394:Molecular Structure; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D012260:Ribonucleases / Q000737:chemistry
D005936:Glucans; D006020:Glycopeptides; D011134:Polysaccharides; D011506:Proteins; C031146:maltoheptaose; D012260:Ribonucleases; C029600:ribonuclease B