[Inhibitive effect of curcumin and amiloride on the fibrosis of rat hepatic stellate cells induced by oxidative stress].
OBJECTIVE
To investigate the effects of antioxidant curcumin and Na+/H+ exchanger inhibitor amiloride on the fibrosis of rat hepatic stellate cells (HSC) induced by oxidative stress in vitro.


METHODS
HSC were incubated with 0.1 mmol/L ferric nitrilotriacetate complex (FeNTA). MTT colorimetry was used for assaying proliferation of HSC. Cytotoxicity was measured by LDH colorimetry. Collagen type I accummulation in the culture media was measured by ELISA. Intracellular malonildialdehyde (MDA), SOD, GSH and GSH-Px levels in the culture media were measured by respective reagent boxes.


RESULTS
HSC incubation with FeNTA resulted in a significant production of intracellular MDA and GSH, associated with decrease of SOD and GSH-PX activity. Exposure of HSC to FeNTA significantly enhanced the number of proliferating HSC and collagen type I levels in the culture medium. All these effects were reversed by the antioxidant curcumin and by the Na+/H+ exchanger inhibitor amiloride. Combining curcumin with amiloride had better effects on the fibrosis of rat hepatic stellate cells induced by oxidative stress than curcumin or amiloride.


CONCLUSIONS
The effects of combination of antifibrosis drugs curcumin and amiloride on different targets of HSC are superior to curcumin or amiloride.
Yang
Weifeng
W
First Military Medical University, Guangzhou 510515.
Chen
Houchang
H
Jiang
Yiping
Y
D000584:Amiloride / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D003474:Curcumin / Q000008:administration & dosage / Q000494:pharmacology*; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects* / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000473:pathology* / Q000517:prevention & control; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley
D000975:Antioxidants; D024042:Collagen Type I; D004365:Drugs, Chinese Herbal; D000584:Amiloride; D003474:Curcumin