A comparative study of induction of estrus and ovulation by three different intravaginal devices in ewes during the non-breeding season.
The aim of the present study was to compare three methods of estrus synchronization in ewes during the non-breeding season. Forty-two ewes were randomly grouped for three treatments with different intravaginal devices for 12 days: Group A) CIDR, Group B) Self-made P sponge, Group C) MAP (medroxyprogesterone acetate) cream sponge. Furthermore, all groups were divided into two treatments with (R) or without ram presence to examine the "ram effect". Blood was collected from all treated ewes, and progesterone (P(4)), estradiol 17-beta (E(2)) and luteinizing hormone (LH) concentrations were measured by enzyme-immunoassay. All ewes showed estrus behavior between Day 0 to 3 after device removal, and the mean onset times of their estrus were 23.0, 33.0 and 21.0 h for Groups AR, BR and CR, respectively. On Day 5 as examined by laparoscopy, the ovulation rates (and number of ovulated ewes) were 1.45 (11/11), 1.25 (12/14) and 1.21 (14/14) for Groups A, B and C, respectively. In Group C, the time to LH surge was significantly (P<0.05) later (32.4 h) than those in Groups A (27.0 h) and B (25.5 h). Ram presence did not affect the number of ovulated ewes, ovulation rate or time to LH surge. The ram introduction group had significantly (P<0.05) lower E(2) concentrations during the period from 0 h to 36 h than the groups without ram presence. These results suggest that the self-made P sponge or MAP cream sponge was effective as well as CIDR, and ram introduction was not necessary, for induction of estrus and ovulation during the non-breeding season.
Iida
Kenji
K
Laboratory of Animal Reproduction, Department of Animal Production Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-0855, Japan.
Kobayashi
Nao
N
Kohno
Hirohide
H
Miyamoto
Akio
A
Fukui
Yutaka
Y
D000818:Animals; D001947:Breeding / Q000379:methods*; D003271:Contraceptive Agents, Female / Q000494:pharmacology*; D003274:Contraceptive Devices, Female; D004958:Estradiol / Q000097:blood; D004971:Estrus / Q000502:physiology*; D005260:Female; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D017258:Medroxyprogesterone Acetate / Q000494:pharmacology*; D010060:Ovulation / Q000502:physiology*; D011374:Progesterone / Q000097:blood; D011897:Random Allocation; D012756:Sheep; D014622:Vaginal Creams, Foams, and Jellies
D003271:Contraceptive Agents, Female; D014622:Vaginal Creams, Foams, and Jellies; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D017258:Medroxyprogesterone Acetate