Direct injection of immature dendritic cells into irradiated tumor induces efficient antitumor immunity.
Although there are several ways to load tumor antigens to DCs, in vitro preparation of tumor antigens and manipulation of DCs are usually required. Therefore, to develop a simple antitumor immunization method, we examined if direct injection of DCs into tumor apoptosed by ionizing IR could induce efficient antitumor immunity. Ionizing IR with 15 Gy induced apoptosis in tumor maximally after 6 hr. Injection of DCs i.t. into IR tumor induced strong cytotoxicity of splenocytes against tumor cells compared to i.t. injection of DCs or ionizing IR of tumor, both of which induced weak cytotoxicity. In an animal study, i.t. injection of DCs into IR tumor induced therapeutic antitumor immunity against a tumor established at a distant site. Moreover, when TNF-alpha or LPS was added as a danger/maturation signal to DC suspension before i.t. injection, antitumor immunity was significantly potentiated compared to a group treated with i.t. injection of DCs into IR tumor. Our results suggest that injection of DCs into tumor apoptosed by ionizing IR might be a simple and efficient method of immunization against tumor.
Kim
Kwang-Woon
KW
Department of Biochemistry, College of Medicine, Pusan National University, Pusan 602-739, Korea.
Kim
Sun-Hee
SH
Shin
Jin-Gue
JG
Kim
Gui-Sook
GS
Son
Young-Ok
YO
Park
Soon-Won
SW
Kwon
Byung-Hyun
BH
Kim
Dong-Won
DW
Lee
Chang-Hun
CH
Sol
Mee-Young
MY
Jeong
Min-Ho
MH
Chung
Byung-Seon
BS
Kang
Chi-Dug
CD
D000230:Adenocarcinoma / Q000532:radiotherapy; D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000532:radiotherapy; D003713:Dendritic Cells / Q000276:immunology; D005260:Female; D005354:Fibrosarcoma / Q000532:radiotherapy; D015552:Injections, Intralesional; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000276:immunology; D008223:Lymphoma / Q000532:radiotherapy; D051379:Mice; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000276:immunology* / Q000532:radiotherapy*; D016499:Radioimmunotherapy / Q000379:methods*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000008:administration & dosage / Q000276:immunology
D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha