Detection and quantitation of genetically modified maize (Bt-176 transgenic maize) by applying ligation detection reaction and universal array technology.
We have applied the ligation detection reaction (LDR) combined with a universal array approach to the detection and quantitation of the polymerase chain reaction (PCR) amplified cry1A(b) gene from Bt-176 transgenic maize. We demonstrated excellent specificity and high sensitivity. Down to 0.5 fmol (nearly 60 pg) of PCR amplified transgenic material was unequivocally detected with excellent linearity within the 0.1-2.0% range with respect to wild-type maize. We suggest the feasibility of extending the LDR/universal array format to detect in parallel several transgenic sequences that are being developed for food applications.
Consiglio Nazionale delle Ricerche, Istituto di Tecnologie Biomediche and Dipartimento di Scienze e Tecnologie Biomediche, Università degli Studi di Milano, LITA, Via F.lli Cervi, 93 20090 Segrate, Milan, Italy.
Roberta
R
Bordoni
Alessandra
A
Mezzelani
Clarissa
C
Consolandi
Andrea
A
Frosini
Ermanno
E
Rizzi
Bianca
B
Castiglioni
Claudia
C
Salati
Nelson
N
Marmiroli
Rosangela
R
Marchelli
Luigi
L
Rossi Bernardi
Cristina
C
Battaglia
Gianluca
G
De Bellis
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins; D004247:DNA / Q000032:analysis; D011088:DNA Ligases / Q000378:metabolism; D004731:Endotoxins / Q000235:genetics; D006460:Hemolysin Proteins; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D003313:Zea mays / Q000235:genetics*
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D004247:DNA; D011088:DNA Ligases