Adhesion and proliferation of cells on new polymers modified biomaterials.
Up to today, several techniques have been used to maintain cells in culture for studying many aspects of cell biology and physiology. More often, cell culture is dependent on proper anchorage of cells to the growth surface. Poly-l-lysine is commonly used as adhesive molecule. In this study, we present, as an alternative to poly-l-lysine, new polymer film substrates, realized by electropolymerization of different monomers on fluorine-doped tin oxide (FTO) surfaces since electropolymerization is a good method to coat selectively metallic or semiconducting electrodes with polymer films. So, the adhesion, proliferation and morphology of rat neuronal cell lines were investigated on polymer treated surfaces. Several amine-based biocompatible polymers were tested: polyethyleneimine (PEI), polypropyleneimine (PPI), polypyrrole (PPy) and poly(p-phenylenediamine) (PPPD). These polymer films were coated on FTO surfaces by electrochemical oxidation. After 8 h in a culture medium, a high percentage of cells was found to be attached to PEI and PPI compared to the other polymers and to the reference surfaces (glass and FTO uncovered). After 24 and 72 h in the culture medium, cells were found to proliferate faster on PEI and PPI than on other polymers and reference surfaces. Consequently, cells have a greater fold expansion on PEI and PPI than on PPPD, PPy or glass and FTO uncoated. From these results, we deduce that PEI and PPI can be useful as coating surface to cultivate neuronal cells.
Laboratory of Chemistry Materials and Interfaces, UFR Sciences et Technique, University of Franche-Comté, 16 route de Gray, Batiment Propedeutique, 25030 Besançon, France. sophie.lakard@univ-fcomte.fr
S
S
Lakard
G
G
Herlem
A
A
Propper
A
A
Kastner
G
G
Michel
N
N
Vallès-Villarreal
T
T
Gharbi
B
B
Fahys
D000588:Amines / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002478:Cells, Cultured; D004563:Electrochemistry; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D011094:Polyethyleneimine / Q000737:chemistry / Q000494:pharmacology; D011107:Polylysine / Q000737:chemistry / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D013055:Spectrophotometry, Infrared; D013499:Surface Properties
D000588:Amines; D001672:Biocompatible Materials; D011108:Polymers; D011107:Polylysine; D011094:Polyethyleneimine