Behaviour of the new asbestos amphibole fluor-edenite in different lung cell systems.
The aim of the present research was to determine whether the recently identified and characterized new fibrous amphibole fluoro-edenite may induce a cytopathic response in cultured cells. The final goal was to gain suggestions on the potentiality of fluoro-edenite to be harmful to human beings. Epidemiological studies, in fact, have shown an excess of developing mesothelioma among residents in Biancavilla, a town in eastern Sicily located in the Etna volcanic area. Therefore, we treated human lung fibroblasts, human lung alveolar epithelial cancer cell line A549 and monocyte-macrophage cell line J774 with fluoro-edenite or crocidolite; the latter used as a highly toxic amphibole asbestos reference. Our results show that fluoro-edenite may induce functional modifications and affects some biochemical parameters in tested cell cultures in a concentration and time dependent manner. However, the observed functional modifications induced by fluoro-edenite are generally less dramatic than those induced by crocidolite and more evident on human lung alveolar epithelial cancer cell line A549 with respect to those obtained on human lung fibroblasts or monocyte-macrophage cell line J774. The sequence of the damage is hypothesised to be as follows: at increasing fluoro-edenite concentrations, and/or treatment times, the increase in reactive oxygen species (ROS) production could trigger significant DNA damage in cell cultures, concomitantly with drop in cell metabolism and increase in lactic dehydrogenase release. In conclusion, according to our data, fluoro-edenite appears as a probable carcinogenic agent, responsible for the high incidence of malignant pleural mesothelioma in Biancavilla.
Department of Physiological Sciences, University of Catania, Viale Andrea Doria 6, 95125 Catania, Italy.
Venera
V
Cardile
Marcella
M
Renis
Christian
C
Scifo
Laura
L
Lombardo
Rosario
R
Gulino
Barbara
B
Mancari
Annamaria
A
Panico
D000818:Animals; D017636:Asbestos, Amphibole / Q000378:metabolism / Q000633:toxicity*; D017638:Asbestos, Crocidolite / Q000378:metabolism / Q000633:toxicity; D002460:Cell Line; D002478:Cells, Cultured; D020552:Comet Assay; D003601:Cytotoxicity Tests, Immunologic; D004247:DNA / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D017382:Reactive Oxygen Species / Q000378:metabolism; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
D017636:Asbestos, Amphibole; D017382:Reactive Oxygen Species; D013778:Tetrazolium Salts; D013844:Thiazoles; C492470:fluor-edenite; D017638:Asbestos, Crocidolite; D004247:DNA; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C022616:thiazolyl blue