Liquid chromatographic determination of 4,4'-dinitrocarbanilide, the active component of the infertility agent nicarbazin, in chicken, duck, goose, and snake eggs.
4,4'-Dinitrocarbanilide (DNC) was extracted from chicken, duck, goose, and snake eggs and isolated by reversed-phase liquid chromatography. DNC was detected by ultraviolet absorbance at 347 nm and quantitated by comparison with a calibration standard. Recoveries of DNC from fortified control chicken, duck, goose, and snake egg samples were determined for DNC levels of 0.16, 10, and 16 microg/g. The mean recoveries from chicken, duck, goose, and snake eggs were 92 +/- 4, 88 +/- 9, 87 +/- 7, and 95 +/- 6%, respectively. The method limits of detection for DNC in chicken, duck, goose, and snake eggs ranged from 0.015 to 0.035 microg/g. The reported method is much simpler than and equally efficient as previous methods developed for the determination of DNC residues in egg contents.
Primus
Thomas M
TM
US Department of Agriculture, Animal and Plant Health Inspection Service, National Wildlife Research Center, 4101 LaPorte Ave, Ft Collins, Denver, CO 80521, USA. thomas.m.primus@aphis.usda.gov
Kohler
Dennis J
DJ
Goodall
Margaret A
MA
Yoder
Christi
C
Mathies
Thomas
T
Miller
Lowell
L
Johnston
John J
JJ
Vercauteren
Kurt
K
D000818:Animals; D002138:Calibration; D002224:Carbanilides / Q000032:analysis*; D002645:Chickens; D004372:Ducks; D004531:Eggs / Q000032:analysis*; D005299:Fertility Agents / Q000032:analysis*; D005777:Geese; D007202:Indicators and Reagents; D009528:Nicarbazin / Q000032:analysis*; D012015:Reference Standards; D012911:Snakes; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
D002224:Carbanilides; D005299:Fertility Agents; D007202:Indicators and Reagents; D012996:Solutions; D009528:Nicarbazin; C039493:4,4'-dinitrocarbanilide