Biofilm proteome: homogeneity or versatility?
The problems associated with biofilm infections in humans result from the distinct characteristics of biofilms, in particular their high level of resistance to antibiotics. One of the hypotheses that have been advanced to explain this resistance to antimicrobials is the phenotypic differentiation of biofilm cells. Although many studies on biofilms have highlighted physiological alterations following the attachment of bacteria to a surface, no studies have explicitly demonstrated a "biofilm" physiology. To contribute to this topical debate, we used principal component analysis to interpret spot quantity variations observed on electropherograms obtained by two-dimensional gel electrophoresis of crude protein extracts from planktonic and sessile Pseudomonas aeruginosa cells. These analyses showed that the proteome of attached P. aeruginosa cells differs from that of their planktonic counterparts. Furthermore, we found that the proteome of sessile P. aeruginosa is strongly dependent on the nature of the biofilm substratum.
European Institute for Peptide Research (IFRMP 23), UMR 6522 CNRS, 76821 Mont-Saint-Aignan Cedex, France.
Sébastien
S
Vilain
Pascal
P
Cosette
Irène
I
Zimmerlin
Jean-Paul
JP
Dupont
Guy-Alain
GA
Junter
Thierry
T
Jouenne
D001426:Bacterial Proteins / Q000032:analysis*; D018441:Biofilms; D002448:Cell Adhesion; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005782:Gels; D008855:Microscopy, Electron, Scanning; D015999:Multivariate Analysis; D010933:Plankton; D020543:Proteome / Q000032:analysis*; D011550:Pseudomonas aeruginosa / Q000737:chemistry; D013499:Surface Properties
D001426:Bacterial Proteins; D005782:Gels; D020543:Proteome