Analytical characterisation of crude extracts from an African Ancistrocladus species using high-performance liquid chromatography and capillary electrophoresis coupled to ion trap mass spectrometry.
The analysis by HPLC, CE and CE-MS/MS of root bark extracts of a, so far undescribed, Central-African Ancistrocladus species (family Ancistrocladaceae) is described. Owing to the complexity of the extract, the application of reversed-phase HPLC resulted in a partially incomplete separation of the naphthylisoquinoline alkaloids, whilst CE using a non-aqueous buffer proved to be a very valuable complementary method for a first characterisation of the crude extract. By performing additional CE-MS/MS experiments, in combination with parallel isolation studies and structural elucidation using conventional methods, six alkaloidal substances present in the plant could be identified.
Unger
Matthias
M
Institute of Pharmacy and Food Chemistry, Julius-Maximilians University Würzburg, D-97074 Würzburg, Germany. unger@pharmazie.uni.wuerzburg.de
Dreyer
Michael
M
Specker
Sven
S
Laug
Stefanie
S
Pelzing
Matthias
M
Neusüss
Christian
C
Holzgrabe
Ulrike
U
Bringmann
Gerhard
G
D000470:Alkaloids / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015023:Democratic Republic of the Congo; D019075:Electrophoresis, Capillary / Q000379:methods; D007546:Isoquinolines / Q000302:isolation & purification*; D019684:Magnoliopsida / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry
D000470:Alkaloids; D007546:Isoquinolines; D010936:Plant Extracts