Diffusion constant in gel electrophoresis at high fields.
We present new measurements of the diffusion constant D in standard (slab-gel) electrophoresis of DNA at fields up to 10 V/cm. Molecules investigated are bacteriophages: T4 of length 173 kbp and lambda of length 48.5 kbp cut by restriction enzyme HindIII. We show, that D increases with the molecule length for electric field E above 5 V/cm. The results are interpreted within the geometration model.
Faculty of Physics and Nuclear Techniques, AGH University of Science and Technology, al Mickiewicza 30, PL-30059 Kraków, Poland. kulakowski@novell.ftj.agh.edu.pl
Małgorzata J
MJ
Krawczyk
Józef
J
Dulak
Paweł
P
Paściak
Krzysztof
K
Kułakowski
D017122:Bacteriophage T4 / Q000235:genetics; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry; D004058:Diffusion; D004587:Electrophoresis, Agar Gel
D004279:DNA, Viral; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C024400:GTYRAC-specific type II deoxyribonucleases