Gingival crevicular fluid levels of aspartate amino transferase, sulfide ions and N-benzoyl-DL-arginine-2-naphthylamide in diabetic patients with chronic periodontitis.
BACKGROUND
The aim of this study is to analyze the correlations between plaque index (PlI), gingival index (GI), probable pocket depth (PPD), clinical attachment level (CAL), aspartate aminotransferase (AST), N-benzoyl-DL-arginine-2-naphthylamide (BANA) and sulfide ion activity (SIA) of diabetic patients with chronic periodontitis with regard to disease activity detected by AST levels.


METHODS
A total of 95 sites from eight diabetic patients with chronic periodontitis and 74 sites from eight systemically healthy patients with chronic periodontitis were enrolled in the study. The patients had no history of periodontal treatment or any antibiotic therapy during the last 6 months and were nonsmokers. All the sites selected for the study had a CAL of at least 2 mm. Gingival crevicular fluid volumes (GCFV) were measured in all sites.


RESULTS
According to the result of AST analysis, 45 sites were AST positive and 50 were AST negative in the diabetic group and 36 sites were AST positive and 38 were AST negative in the control group. There was a significant correlation between BANA hydrolysis and PPD in both diabetic and control groups, but no correlation between PPD and AST levels. A significant correlation was observed between AST-positive sites and GI, but not between GI and BANA hydrolysis. In both groups, the correlation between SIA and BANA hydrolysis was significant, but no correlation was revealed between SIA and AST levels in either diabetic or control groups.


CONCLUSIONS
The GCF metabolites had significant correlations with periodontally diseased sites in patients with chronic periodontitis, whether diabetic or systemically healthy, and may help to confirm clinical findings.
Department of Periodontology, School of Dentistry, Istanbul University, Isthanbul, Turkey.
B
B
Yucekal-Tuncer
C
C
Uygur
E
E
Firatli
D001219:Aspartate Aminotransferases / Q000032:analysis*; D001587:Benzoylarginine-2-Naphthylamide / Q000032:analysis*; D016009:Chi-Square Distribution; D002908:Chronic Disease; D003773:Dental Plaque / Q000378:metabolism; D003774:Dental Plaque Index; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism*; D005883:Gingival Crevicular Fluid / Q000737:chemistry* / Q000201:enzymology; D006801:Humans; D017622:Periodontal Attachment Loss / Q000145:classification / Q000378:metabolism; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000145:classification / Q000378:metabolism; D010518:Periodontitis / Q000378:metabolism*; D018709:Statistics, Nonparametric; D013440:Sulfides / Q000032:analysis*
D013440:Sulfides; D001587:Benzoylarginine-2-Naphthylamide; D001219:Aspartate Aminotransferases