Characterization of HCF-1, a determinant of Autographa californica multiple nucleopolyhedrovirus host specificity.
Autographa californica multiple nucleopolyhedrovirus (AcMNPV) infects a wide variety of insect species. A number of AcMNPV late expression factors that are involved in replication have been identified as playing a role in determining host specificity. Host cell factor-1, or HCF-1, was previously demonstrated to be essential for viral replication in Tn-368 cells. Here we demonstrate that HCF-1 is an early protein and localizes to the cell nucleus. Coprecipitation experiments revealed that HCF-1 interacts with itself but none of the other late expression factors required for replication in Tn-368 cells. HCF-1 mutants were constructed and utilized to search for the domains involved in HCF-1 biological function and oligomerization. Possible roles of HCF-1 in determining host specificity are discussed.
Cornell Research Foundation, Cornell University, Ithaca, NY 14850, USA. klh22@cornell.edu
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Hefferon
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D017353:Gene Deletion; D015870:Gene Expression; D051863:Host Cell Factor C1; D015151:Immunoblotting; D010957:Plasmids; D011233:Precipitin Tests; D018411:Spodoptera; D014157:Transcription Factors / Q000235:genetics*; D014162:Transfection; D020798:Two-Hybrid System Techniques; D001616:beta-Galactosidase
D051863:Host Cell Factor C1; D014157:Transcription Factors; D001616:beta-Galactosidase