Dialysis-Chelex method for determination of exchangeable copper in human plasma.
A method based on dialysis in the presence of histidine, and subsequent copper adsorption and preconcentration using Chelex-100 resin, has been developed for selective extraction of exchangeable copper from blood plasma. The method was verified by comparative analysis using two different instrumental techniques, ICP-MS and AAS. The results obtained were in excellent agreement. Accurate quantification of the exchangeable copper and the firmly bound copper was achieved. The method was further validated by analysis of a lyophilised human serum certified reference material.
Department of Analytical Chemistry, Faculty of Chemistry, Sofia University, 1 J. Bourchier Blvd, 1164 Sofia, Bulgaria.
Tony I
TI
Venelinov
Ian M
IM
Davies
John H
JH
Beattie
D003300:Copper / Q000097:blood*; D003956:Dialysis / Q000379:methods*; D006801:Humans; D011137:Polystyrenes / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D012015:Reference Standards; D012709:Serum Albumin / Q000737:chemistry
D011137:Polystyrenes; D011145:Polyvinyls; D012709:Serum Albumin; D003300:Copper; C006960:chelex