Cloning and characterization of the promoter region of human LPTS/PinX1 gene.
The gene for LPTS/PinX1 encodes a potent telomerase inhibitor and suppresses tumor cell growth. In order to investigate the transcriptional regulation of this gene, we isolated its 5'-flanking region from the human genomic BAC clone and identified a major transcriptional initiation site. The sequence of the 5'-flanking region is GC-rich, lacks canonical TATA box, but contains potential binding sites for a variety of transcription factors. The deletion analysis indicated that the proximal 100 bp (from nt -66 to +34) is essential for minimal promoter activity and the regions of promoter from nt -1272 to -573 and nt -330 to -66 are required for maximal expression of the LPTS/PinX1 gene. Four DNase I hypersensitive sites (DHS1-4) mapping to the regions of transcription initiation and promoter in LPTS/Pinx1 gene were also revealed.
Wang
Suiquan
S
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China.
Liao
Cheng
C
Li
Tsaiping
T
Zhao
Mujun
M
D001483:Base Sequence; D001665:Binding Sites; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular; D003850:Deoxyribonuclease I / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D017421:Sequence Analysis; D024363:Transcription Initiation Site; D025521:Tumor Suppressor Proteins / Q000737:chemistry / Q000235:genetics*
D018797:Cell Cycle Proteins; C440808:PINX1 protein, human; D011506:Proteins; D025521:Tumor Suppressor Proteins; D003850:Deoxyribonuclease I