Identification and distinct regulation of yeast TATA box-containing genes.
Despite being one of the first eukaryotic transcriptional regulatory elements identified, the sequence of a native TATA box and its significance remain elusive. Applying criteria associated with TATA boxes we queried several Saccharomyces genomes and arrived at the consensus TATA(A/T)A(A/T)(A/G). Approximately 20% of yeast genes contain a TATA box. Strikingly, TATA box-containing genes are associated with responses to stress, are highly regulated, and preferentially utilize SAGA rather than TFIID when compared to TATA-less promoters. Transcriptional regulation in yeast appears to be mechanistically bipolar, possibly reflecting a need to balance inducible stress-related responses with constitutive housekeeping functions.
Basehoar
Andrew D
AD
Graduate Program in Statistics, The Pennsylvania State University, University Park, PA 16802, USA.
Zanton
Sara J
SJ
Pugh
B Franklin
BF
D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D016384:Consensus Sequence; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D006801:Humans; D010802:Phylogeny; D012440:Saccharomyces / Q000235:genetics*; D016385:TATA Box; D019473:Transcription Factors, TFII / Q000378:metabolism; D014158:Transcription, Genetic
D002843:Chromatin; D019473:Transcription Factors, TFII