A tiling resolution DNA microarray with complete coverage of the human genome.
We constructed a tiling resolution array consisting of 32,433 overlapping BAC clones covering the entire human genome. This increases our ability to identify genetic alterations and their boundaries throughout the genome in a single comparative genomic hybridization (CGH) experiment. At this tiling resolution, we identified minute DNA alterations not previously reported. These alterations include microamplifications and deletions containing oncogenes, tumor-suppressor genes and new genes that may be associated with multiple tumor types. Our findings show the need to move beyond conventional marker-based genome comparison approaches, that rely on inference of continuity between interval markers. Our submegabase resolution tiling set for array CGH (SMRT array) allows comprehensive assessment of genomic integrity and thereby the identification of new genes associated with disease.
British Columbia Cancer Research Centre, 601 West 10th Avenue, Vancouver, British Columbia V5Z 1L3, Canada.
Adrian S
AS
Ishkanian
Chad A
CA
Malloff
Spencer K
SK
Watson
Ronald J
RJ
DeLeeuw
Bryan
B
Chi
Bradley P
BP
Coe
Antoine
A
Snijders
Donna G
DG
Albertson
Daniel
D
Pinkel
Marco A
MA
Marra
Victor
V
Ling
Calum
C
MacAulay
Wan L
WL
Lam
D022202:Chromosomes, Artificial, Bacterial; D018628:Gene Dosage; D015894:Genome, Human; D006801:Humans; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured