In vivo PLT increments after transfusions of WBC-reduced PLT concentrates stored for up to 7 days.
BACKGROUND
Bacterial screening and improvement of storage conditions of leukoreduced PLT concentrates (LR-PCs) allows extension of their storage period from 5 to 7 days.


METHODS
For in vitro studies, 40 LR-PCs made from five buffy coats and plasma were studied for 8 days. For in vivo studies, routinely produced LR-PCs stored for 2 to 7 days after blood collection were administered to clinically stable thrombocytopenic patients. CI1 h was calculated after 353 transfusions (67 patients), and CCI1 h, after 195 transfusions (55 patients), with pretransfusion PLT counts of not greater than 20 x 10(9) per mL.


RESULTS
Storage experiments showed that the pH of LR-PCs remained greater than 6.8 for 8 days, provided that the PLT concentration was less than 1.3 x 10(9) per mL. Routinely produced LR-PCs had a volume of 282 +/- 15 mL (n = 10,193) and contained 329 x 10(9)+/- 40 x 10(9) PLTs (n = 3467). For 7-day-old LR-PCs, 76 of 78 (97%) of the transfusions resulted in a CI1 h of at least 10 and 37 of 39 (95%) in a CCI1 h of at least 7.5, which indicated levels for successfulness. Mean +/- SE values of CI1 h and CCI1 h of 7-day-old LR-PCs were 28.7 +/- 2.3 (n = 78) and 19.0 +/- 2.0 (n = 39), respectively. No significant differences were observed between 5- and 7-day-old LR-PCs transfused with respect to CI1 h and CCI1 h values.


CONCLUSIONS
In vitro and in vivo studies showed that LR-PCs can be stored for up to 7 days with excellent clinical results, provided that they are routinely screened for bacterial contamination.
Sanquin Blood Bank Northwest Region, Plesmanlaan 125, 1066 CX Amsterdam, the Netherlands. m.dijkstra@sanquin.nl
M J
MJ
Dijkstra-Tiekstra
R N I
RN
Pietersz
E C M
EC
Hendriks
H W
HW
Reesink
P C
PC
Huijgens
D001424:Bacterial Infections / Q000517:prevention & control*; D001793:Blood Preservation; D005260:Female; D006801:Humans; D007962:Leukocytes; D008297:Male; D010976:Platelet Count; D017713:Platelet Transfusion; D013921:Thrombocytopenia / Q000628:therapy*