Synthesis, crystal structure and DNA cleavage activities of copper(II) complexes with asymmetric tridentate ligands.
Two asymmetric tridentate copper(II) complexes, [Cu(dppt)Cl(2)].0.25H(2)O (1) (dppt=3-(1,10-phenanthrolin-2-yl)-5,6-diphenyl-as-triazine) and [Cu(pta)Cl(2)] (2) (pta=3-(1,10-phenanthrolin-2-yl)-as-triazino[5,6-f]acenaphthylene), have been prepared and characterized by elemental analysis, IR and Fast atomic bombardment mass spectra. Complex 1 has also been structurally characterized. The complexes exist as distorted square pyramid with five co-ordination sites occupied by the tridentate ligand and the two chlorine anions. DNA interaction studies suggest that the ligand planarity of the complex has a significant effect on DNA binding affinity increasing in the order [Cu(dppt)Cl(2)]< [Cu(pta)Cl(2)]. In the presence of ascorbate or glutathione, the two complexes are found to cause significant cleavage of double-strand pBR 322 DNA and [Cu(pta)Cl(2)] exhibited the higher cleaving efficiency.
Wang
Xiang-Li
XL
Department of Chemistry, Sun Yat-Sen University, Guangzhou 510275, PR China.
Chao
Hui
H
Li
Hong
H
Hong
Xian-Lan
XL
Ji
Liang-Nian
LN
Li
Xiao-Yuan
XY
D000818:Animals; D002417:Cattle; D003300:Copper / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004563:Electrochemistry / Q000379:methods; D008024:Ligands; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D010618:Phenanthrolines / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013055:Spectrophotometry, Infrared; D014227:Triazines / Q000737:chemistry
D008024:Ligands; D009942:Organometallic Compounds; D010618:Phenanthrolines; D014227:Triazines; D003300:Copper; D004247:DNA; C427203:calf thymus DNA; C025205:1,10-phenanthroline