Sporozoite and liver stage antigen Plasmodium falciparum peptides bind specifically to human hepatocytes.
Sporozoite and Liver Stage Antigen (SALSA) sequence synthetic peptides were used in HepG2 cell binding assays to identify regions involved in parasite invasion. SALSA 20608 ( 21IWASEKKDEKEASEQGEESHY40) and 20611 ( 64KKDDGTDKVQEKVLEKSPKY83) peptides were determined as having high binding activity in HepG2 cell assays, some of them were located in immunogenic regions. Immune-fluorescence antibody test with 24276 (20608 peptide analogue, CGIWSSMKMDEKMAAMQGEESHCG) showed sporozoite and merozoite reactivity. This data suggests SALSA high activity binding peptides' (HABPs) possible role in hepatic cell invasion and merozoite invasion of erythrocytes.
Fundacion Instituto de Inmunología de Colombia and Universidad Nacional de Colombia, Carrera 50 # 26-00, Bogotá, Colombia.
Alvaro
A
Puentes
Javier
J
García
Ricardo
R
Vera
Ramsés
R
López
Jorge
J
Suarez
Luis
L
Rodríguez
Hernando
H
Curtidor
Marisol
M
Ocampo
Diana
D
Tovar
Martha
M
Forero
Adriana
A
Bermudez
Jimena
J
Cortes
Mauricio
M
Urquiza
Manuel E
ME
Patarroyo
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology* / Q000378:metabolism*; D001029:Aotus trivirgatus; D001667:Binding, Competitive; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000469:parasitology; D019084:Fluorescent Antibody Technique, Indirect; D022781:Hepatocytes / Q000378:metabolism* / Q000469:parasitology; D006801:Humans; D007553:Isotope Labeling; D008099:Liver / Q000469:parasitology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000378:metabolism*; D010963:Plasmodium falciparum / Q000276:immunology* / Q000378:metabolism*; D011485:Protein Binding; D034101:Sporozoites / Q000276:immunology*
D000953:Antigens, Protozoan; D010455:Peptides