[Effects of epidermal growth factor on calciumion and cAMP in the ductal cells of submandibular glands].
OBJECTIVE
To study the effects of EGF on Ca(2+) and cAMP in the ductal cells of rat SMG.


METHODS
Fluorometric technique and radioimmunological technique were used to investigate the dynamic changes of Ca(2+) and cAMP in the ductal cells. The data were analysed statistically by t test.


RESULTS
After an momently latent period, intercellular calcium increased rapidly, then began to decline. This period could last 5 minutes. After 3 minutes, cAMP showed a rapid decrease, and then increased slowly. Its concentration could restore 90 minutes later.


CONCLUSIONS
EGF may regulate intercellular calcium and cAMP, which results in the proliferation of the ductal cells.
Department of Oral and Maxillofacial Surgery, the First Affilliated Hospital, China Medical University, Shenyang 110002, China.
Shao-hui
SH
Huang
Yu-rong
YR
Kou
Xue-xin
XX
Tan
Qin
Q
Zhou
Chun-fu
CF
Deng
Yu-xin
YX
Wang
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002455:Cell Division; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013363:Submandibular Gland / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D004815:Epidermal Growth Factor; D000242:Cyclic AMP; D002118:Calcium