Cdk inhibitor ste9p/srw1p is involved in response to protein synthesis inhibition in fission yeast.
It remains unknown whether the cell cycle system responds properly to protein synthesis inhibition. In this paper I report finding in Schizosaccharomyces pombe that partially deleted elongation factor 3 genes rescue various mitotic catastrophe mutants depending on deltaste9 in a dominant-negative manner. In response to protein synthesis inhibitors, deltaste9 and some other mutants delay halting the cell cycle at G2-M and the combined cdc2-M26 deltaste9 mutant greatly loses viability. It is suggested that cell cycle be positively controlled in an ste9-dependent manner before essential factors for viability and other important functions are exhausted when protein synthesis is inhibited.
Sakai
Takaaki
T
Department of Biochemistry and Molecular Biology, Graduate School of Medicine, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan. tsakai@m.u-tokyo.ac.jp
D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors* / Q000235:genetics; D003513:Cycloheximide / Q000494:pharmacology; D005434:Flow Cytometry; D005656:Fungal Proteins / Q000502:physiology; D016195:G2 Phase / Q000187:drug effects / Q000502:physiology; D017353:Gene Deletion; D018816:Genes, cdc; D006921:Hygromycin B / Q000494:pharmacology; D016296:Mutagenesis; D010445:Peptide Elongation Factors / Q000502:physiology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D013489:Suppression, Genetic
D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D010445:Peptide Elongation Factors; D011500:Protein Synthesis Inhibitors; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C111584:YEF3 protein, S cerevisiae; C113000:srw1 protein, S pombe; D006921:Hygromycin B; D003513:Cycloheximide; D018844:Cyclin-Dependent Kinases