Stir bar sorptive extraction-liquid desorption applied to the analysis of hop-derived bitter acids in beer by micellar electrokinetic chromatography.
Stir bar sorptive extraction-liquid desorption (SBSE-LD) has been applied as an efficient sample preparation method for the analysis of beer bitter acids. Extracts free of almost all interfering compounds were obtained, allowing simultaneous analysis of iso-alpha-acids and reduced iso-alpha-acids. A robust micellar electrokinetic chromatography (MEKC) method was developed that enables fast separation of iso-alpha-acids and reduced iso-alpha-acids. Quantitative data are in good agreement with results obtained by high-performance liquid chromatography (HPLC) using direct beer injection.
University of Stellenbosch, Department of Chemistry, CENSSUS, Matieland, South Africa.
André
A
De Villiers
Gerd
G
Vanhoenacker
Fréderic
F
Lynen
Pat
P
Sandra
D000143:Acids / Q000032:analysis* / Q000737:chemistry; D000327:Adsorption; D001515:Beer / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D027582:Humulus / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013237:Stereoisomerism
D000143:Acids; D012997:Solvents