Detection of K-ras gene mutation in fecal samples from elderly large intestinal cancer patients and its diagnostic significance.
OBJECTIVE
To study the diagnostic significance of K-ras gene mutations in fecal samples from elderly patients with large intestinal cancer.


METHODS
DNA was extracted in the fecal and tissue samples from 23 large intestinal cancer patients, 20 colonic adenomatoid polypus patients and 20 healthy subjects. The K-ras gene mutations at the first and second bases of codon 12 were detected by the allele specific mismatch method.


RESULTS
The K-ras gene mutation was 56.52%(13/23) in the large intestinal cancer patients, which was notably higher than that in the normal subjects whose K-ras gene mutation was 5%(1/20) (chi (2)=12.93, P<0.001). There was no significant difference in comparison with that of colonic adenomatoid polypus patients whose K-ras gene mutation was 30%(6/12) (chi (2)=3.05, P>0.05). The K-ras gene mutation at the second base of codon 12 was 92.13%(12/13) in the large intestinal cancer patients. There was no significant difference between the detection rate of K-ras gene mutation in the fecal and tissue samples (chi (2)=9.35, P<0.01).


CONCLUSIONS
Our results indicate that detection of the K-ras gene mutations in fecal samples provides a non-invasive diagnostic method for the elderly large intestinal cancer patients. Its significance in the early diagnosis of large intestinal cancer awaits further studies.
Wan
Jun
J
Department of Geriatric Gastroenterology, South Building of General Hospital of the Chinese PLA, Beijing, China. wanjun@301hospital.com.cn
Zhang
Zi-Qi
ZQ
You
Wei-Di
WD
Sun
Hua-Kui
HK
Zhang
Jian-Ping
JP
Wang
Ya-Hong
YH
Fu
Yong-He
YH
D000368:Aged; D003110:Colonic Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D003111:Colonic Polyps / Q000175:diagnosis* / Q000235:genetics*; D005243:Feces; D005260:Female; D011905:Genes, ras / Q000235:genetics*; D005820:Genetic Testing / Q000379:methods; D006801:Humans; D008297:Male; D008403:Mass Screening / Q000379:methods; D009154:Mutation