TNF-alpha, H2O2 and NO response of peritoneal macrophages to Yersinia enterocolitica O:3 derivatives.
In this study, the effect of Yersinia derivatives on nitric oxide (NO), hydrogen peroxide (H2O2) and tumor necrosis factor-alpha (TNF-alpha) production by murine peritoneal macrophages was investigated. Addition of lipopolysaccharide (LPS) to the macrophage culture resulted in NO production that was dose dependent. On the other hand, bacterial cellular extract (CE) and Yersinia outer proteins (Yops) had no effect on NO production. The possible inhibitory effect of Yops on macrophage cultures stimulated with LPS was investigated. Yops partially inhibited NO production (67.4%) when compared with aminoguanidine. The effects of Yersinia derivatives on H2O2 production by macrophages were similar to those on NO production. LPS was the only derivative that stimulated H2O2 release in a dose-dependent manner. All Yersinia derivatives provoked the production of TNF-alpha, but LPS had the strongest effect, as observed for NO production. CE and Yops stimulated TNF-alpha production to a lesser extent than LPS. The results indicate the possibility that in vivo Yops may aid the evasion of the bacteria from the host defense mechanism by impairing the secretion of NO by macrophages.
Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas de Araraquara, UNESP, Rua Expedicionários do Brasil, No. 1621, Araraquara, SP, CEP 14.801-902, Brazil. carlosiz@fcfar.unesp.br
Iracilda Zeppone
IZ
Carlos
Luis Gustavo
LG
Silva Monnazzi
Deise Pasetto
DP
Falcão
Beatriz Maria
BM
Machado de Medeiros
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D005260:Female; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008262:Macrophage Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000378:metabolism / Q000382:microbiology*; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000378:metabolism*; D015008:Yersinia enterocolitica / Q000276:immunology / Q000502:physiology*
D001425:Bacterial Outer Membrane Proteins; D014409:Tumor Necrosis Factor-alpha; C078909:YopB protein, Yersinia; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse