Novel anthraquinone conjugate of 2,2-bis(hydroxymethyl)propionic acid incorporated to a TFO with phosphodiester linkage facilitates triplex formation with dsDNA bearing a pyrimidine-gapped polypurine sequence.
An anthraquinone derivative conjugated with 2,2-bis(hydroxymethyl)propionic acid as a novel non-nucleosidic component was synthesized and successfully incorporated into an internal region of 14-mer triplex-forming oligonucleotide (TFO) via the phosphoramidite method. The resulted novel TFO exhibits remarkable enhancement effect on the thermal stability of a DNA triplex upon binding to a pyrimidine-gap containing polyprurine sequence.
Department of Chemistry, Faculty of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu 376-8515, Japan.
Megumi
M
Sato
Tomohisa
T
Moriguchi
Kazuo
K
Shinozuka
D000880:Anthraquinones / Q000737:chemistry*; D001483:Base Sequence / Q000235:genetics; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D006880:Hydroxy Acids; D009841:Oligonucleotides / Q000737:chemistry* / Q000235:genetics; D011422:Propionates / Q000737:chemistry*; D011687:Purines / Q000737:chemistry; D011743:Pyrimidines / Q000737:chemistry*
D000880:Anthraquinones; D006880:Hydroxy Acids; D009841:Oligonucleotides; D011422:Propionates; D011687:Purines; D011743:Pyrimidines; C051802:triplex DNA; C110034:2,2-bis(hydroxymethyl)-propionic acid; D004247:DNA