Simultaneous detection of seven mutations with seven forward primers and one common reverse primer in a single PCR step.
A new PCR method is described here to amplify simultaneously several fragments sharing the same template in a single PCR step with a hemi-primer. Using this method, we successfully detected various point mutations in tissue plasminogen activator (tPA) mutants.
Wang
Qiang
Q
Biotechnology Research Center and Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences, Zhongshan University, Canton 510275, PR China.
Liu
Qiuyun
Q
Li
Bao-jian
BJ
D004252:DNA Mutational Analysis; D017931:DNA Primers / Q000737:chemistry*; D018076:DNA, Complementary / Q000378:metabolism; D005821:Genetic Techniques; D006801:Humans; D009154:Mutation; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D010959:Tissue Plasminogen Activator / Q000235:genetics
D017931:DNA Primers; D018076:DNA, Complementary; D010959:Tissue Plasminogen Activator