Rat mutations cvd and hob with cerebellar malformations map to chromosome 2.
In this paper, we executed genome mapping and comparative mapping analyses for cvd and hob, autosomal recessive mutations with cerebellar vermis defect and cerebellar dysplasia in the rat. For the linkage analysis, we produced three sets of backcross progeny, (ACI x CVD)F(1) and (F344 x CVD)F(1) females crossed to a cvd homozygous male rat, and (HOB x WKY)F(1) males crossed to hob homozygous female rats. Analysis of the segregation patterns of simple sequence length polymorphism (SSLP) markers scanning the whole rat genome allowed the mapping of these autosomal recessive mutations to rat Chromosome (Chr) 2. The most likely gene order is D2Mgh12 - D2Rat86 - D2Mit15 - D2Rat185 - cvd - D2Rat66 - D2Mgh13, and D2Mit18 - Fga -D2Mit14 - D2Rat16 - hob - D2Mgh13. Crossing test between a proven cvd heterozygous and a hob heterozygous rats demonstrated their allelism. Furthermore, comparative mapping indicated the cvd locus corresponds to mouse chromosome 3 and a strong candidate gene Unc5h3, a causative gene for the rostral cerebellar malformation mouse, was implicated.
Laboratory of Veterinary Pathology, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University.
Mitsuru
M
Kuwamura
Yosuke
Y
Ando
Akiko
A
Takada
Toshiko
T
Kanehara
Jyoji
J
Yamate
Takao
T
Kotani
Shigehito
S
Takeshita
Miyuki
M
Kanbori
Kazuhiro
K
Kitada
Tadao
T
Serikawa
D000483:Alleles; D000818:Animals; D002531:Cerebellum / Q000002:abnormalities*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D005260:Female; D023061:Gene Order; D005819:Genetic Markers; D006239:Haplotypes / Q000235:genetics; D008297:Male; D000075386:Netrin Receptors; D011110:Polymorphism, Genetic; D051381:Rats; D011956:Receptors, Cell Surface / Q000235:genetics
D005819:Genetic Markers; D000075386:Netrin Receptors; D011956:Receptors, Cell Surface; C105653:Unc5c protein, mouse