Disruption of basement membrane, extracellular matrix metalloproteinases and E-cadherin in renal-cell carcinoma.
A retrospective study was performed to determine the prognostic value of Basement Membrane (BM) integrity, Matrix Metalloproteinases (MMPs) and E-Cadherin expression in renal cell carcinoma (RCC).


METHODS
An immunohistochemical study on laminin and collagen IV, MMPs 1 and 2, and E-Cadherin was carried out on 71 RCCs. BM fragmentation was considered taking 75% as a cut-off. MMP 1 and MMP2 immunostaining, as well as E-Cadherin was considered taking 25% as a cut-off.


RESULTS
An inverse relationship was seen between E-Cadherin with laminin, collagen IV and MMPs. More than 75% loss of laminin, collagen IV and E-Cadherin, as well as higher expression of MMPs, were associated with symptoms, tumoral size and worse grade. Loss of collagen IV and E-Cadherin were of prognostic value.


CONCLUSIONS
Both BM and E-Cadherin are good prognostic markers. MMPs patterns show a relationship between BM proteins and E-Cadherin, but evaluation is more time-consuming and provide no better prognostication; consequently they are not useful in routine clinical applications.
Department of Pathology, Medical School of Valencia, Avda. Blasco Ibanez, 17, 46010 Valencia, Spain.
L
L
Morell-Quadreny
Jose
J
Rubio
Jose Antonio
JA
Lopez-Guerrero
Juan
J
Casanova
D
D
Ramos
Inmaculada
I
Iborra
Eduardo
E
Solsona
A
A
Llombart-Bosch
D001485:Basement Membrane / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D015820:Cadherins / Q000096:biosynthesis; D002292:Carcinoma, Renal Cell / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D024141:Collagen Type IV / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D007797:Laminin / Q000096:biosynthesis; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis; D008565:Membrane Proteins / Q000096:biosynthesis*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009367:Neoplasm Staging; D011379:Prognosis
D014408:Biomarkers, Tumor; D015820:Cadherins; D024141:Collagen Type IV; D007797:Laminin; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D020778:Matrix Metalloproteinase 2; D020781:Matrix Metalloproteinase 1