Irradiation attenuates neurogenesis and exacerbates ischemia-induced deficits.
Increased neurogenesis after cerebral ischemia suggests that functional recovery after stroke may be attributed, in part, to neural regeneration. In this study, we investigated the role of neurogenesis in the behavioral performance of gerbils after cerebral global ischemia. We used ionizing radiation to decrease neural regeneration, and 2 weeks later cerebral global ischemia was induced by bilateral common carotid artery occlusion. One month after the occlusion, the animals were behaviorally tested. Irradiation alone reduced neurogenesis but did not change vascular or dendritic morphology at the time of behavioral testing. Neither did irradiation, ischemia, or combined treatment impair rotor-rod performance or alter open-field activity. Gerbils subjected to both irradiation and ischemia demonstrated impaired performance in the water-maze task, compared with those that received only ischemia, radiation, or no treatment. These impairments after cerebral global ischemia under conditions of reduced neurogenesis support a role for the production of new cells in mediating functional recovery.
Raber
Jacob
J
Departments of Behavioral Neuroscience and Neurology, Oregon Health and Science University, Portland, OR, USA.
Fan
Yang
Y
Matsumori
Yasuhiko
Y
Liu
Zhengyan
Z
Weinstein
Philip R
PR
Fike
John R
JR
Liu
Jialing
J
D000818:Animals; D001522:Behavior, Animal / Q000528:radiation effects; D001562:Benzimidazoles / Q000378:metabolism; D002545:Brain Ischemia / Q000503:physiopathology*; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count; D004924:Escape Reaction / Q000528:radiation effects; D005106:Exploratory Behavior / Q000528:radiation effects; D005849:Gerbillinae; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000528:radiation effects; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D018782:Maze Learning / Q000528:radiation effects; D051379:Mice; D009474:Neurons / Q000502:physiology / Q000528:radiation effects*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D011597:Psychomotor Performance / Q000528:radiation effects; D011839:Radiation, Ionizing; D011930:Reaction Time / Q000528:radiation effects; D012038:Regeneration / Q000528:radiation effects*; D016622:Silver Staining / Q000379:methods; D013997:Time Factors; D014404:Tubulin / Q000378:metabolism
D001562:Benzimidazoles; D005904:Glial Fibrillary Acidic Protein; D014404:Tubulin; C462941:beta3 tubulin, mouse; D010751:Phosphopyruvate Hydratase; D001973:Bromodeoxyuridine; C017807:bisbenzimide ethoxide trihydrochloride