Expression and localization of galectin 4 in rat stomach during postnatal development.
Galectins are lectins implicated in cell-cell or cell-matrix adhesion, cell growth, the cell cycle, transcription processes, and apoptosis, and some of them are differentially regulated during pre- or post-natal development. The purpose of the present study was to determine whether the expression of galectin 4 is relevant to developmental processes during postnatal development in the rat stomach. Galectin 4 expression in the rat gastric mucosa, between birth and adulthood, was studied at the protein and mRNA levels by western and northern blotting, respectively. This lectin was localized precisely by immunoelectron microscopy. In the gastric mucosa, galectin 4 protein was present at lower levels in suckling than in weaned rats, but mRNA levels did not change significantly during postnatal development. This suggests possible differences in mRNA stability or in the translation regulation. Immunocytochemical examination of galectin 4 confirmed more highly elevated levels of the protein in endocrine, parietal, and chief cells in weaned rats than in suckling rats. Galectin 4 was more strongly localized in weaned rats than in suckling rats in the nuclei of all cell types and in or over secretory granules in endocrine and chief cells, suggesting that galectin 4 is implicated in nuclear events and perhaps in secretory processes.
Département de Biochimie, Unité INSERM 189-alliée CNRS, Faculté de Médecine Lyon-Sud, BP 12, 69600 Oullins, France.
Elodie
E
Niepceron
Fabienne
F
Simian-Lermé
Pierre
P
Louisot
Marie-Claire
MC
Biol-N'garagba
D000818:Animals; D037541:Galectin 4 / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D015870:Gene Expression; D007120:Immunochemistry; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013270:Stomach / Q000254:growth & development* / Q000648:ultrastructure
D037541:Galectin 4; D012333:RNA, Messenger