PCR-based genotyping of the rat Atrn(mv) mutation.
Rat myelin vacuolation mutation at the Attractin locus (Atrn(mv)) is a genomic deletion including the whole exon 1 of the Atrn gene. The precise size and location of the deleted region has not yet been identified because of poor information on genomic organization of the rat Atrn gene. Here, we identified the breakpoints of the Atrn(mv) mutation, using a draft sequence of the rat genome. In the Atrn(mv/mv) rat, a 6,914-bp genomic region was deleted. Primers flanked 5'- and 3'- breakpoints amplified the Atrn(mv) allele but not the wild-type allele. This primer set enables us to distinguish Atrn(mv/+) heterozygous rats from Atrn(+/+) rats, and will contribute to the efficient production of Atrn(mv/mv) rats.
Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Satoko
S
Tokuda
Takashi
T
Kuramoto
Tadao
T
Serikawa
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004586:Electrophoresis; D005091:Exons / Q000235:genetics; D017353:Gene Deletion; D016678:Genome; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats / Q000235:genetics*; D016324:Sequence Tagged Sites
C492191:Atrn protein, rat; D017931:DNA Primers; D008565:Membrane Proteins