Laboratory confirmation of generalized vaccinia following smallpox vaccination.
The reinitiation of smallpox vaccination has renewed interest in implementing modern diagnostic methods to assess orthopoxvirus infection and adverse events following vaccination. We report here the laboratory confirmation of vaccinia virus in pustular lesions of a healthy adult vaccinee by use of a two-tier algorithm incorporating TaqMan PCR and electron microscopy.
Kelly
Cassandra D
CD
Wadsworth Center, New York State Department of Health, Albany, New York 12208, USA.
Egan
Christina
C
Davis
Stephen W
SW
Samsonoff
William A
WA
Musser
Kimberlee A
KA
Drabkin
Peter
P
Miller
James R
JR
Taylor
Jill
J
Cirino
Nick M
NM
D000328:Adult; D005260:Female; D016679:Genome, Viral; D006282:Health Personnel; D014645:Herpesvirus 3, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D009519:New York City; D016133:Polymerase Chain Reaction / Q000379:methods; D012882:Skin Tests; D012900:Smallpox Vaccine / Q000009:adverse effects*; D014615:Vaccinia / Q000209:etiology / Q000473:pathology*
D012900:Smallpox Vaccine