Effect of alendronate on osteoclast differentiation and bone volume in transplanted bone.
Alendronate, one of the bisphosphonates, is known to have an inhibitory effect on bone resorption. The purpose of this study was to investigate the effects of alendronate on ectopic bone graft resorption and to determine the optimal dose in the mouse. The grafted bone in the control group disappeared due to resorption by osteoclasts within 5 weeks. In the experimental groups, the area of bone tissue decreased by only 20-40% at 5 weeks post-operatively. At 8 and 9 weeks after surgery, the decreased area of bone structure was significantly less in all the 10(-4) M injected alendronate-immersed groups than in the 10(-4) M non-injected alendronate-immersed. At 9 weeks after surgery, the number of osteoclasts were significantly less in the 10(-4) M injected alendronate-treated groups than in the 10(-4) M non-injected alendronate-treated groups. These results suggest that alendronate inhibits resorption of ectopic bone graft at concentrations of 10(-4) and 10(-6) M.
Department of Orthodontics and Craniofacial Developmental Biology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
Toshitsugu
T
Kawata
Kaoru
K
Tenjou
Chiyoko
C
Tokimasa
Tadashi
T
Fujita
Masato
M
Kaku
Akira
A
Matsuki
Shinya
S
Kohno
Keisuke
K
Tsutsui
Junji
J
Ohtani
Masahide
M
Motokawa
Mao
M
Shigekawa
Yuiko
Y
Tohma
Kazuo
K
Tanne
D019386:Alendronate / Q000494:pharmacology*; D000818:Animals; D001862:Bone Resorption; D016025:Bone Transplantation / Q000473:pathology*; D002454:Cell Differentiation / Q000187:drug effects*; D006651:Histocytochemistry; D051379:Mice; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects*
D019386:Alendronate