Search for serum protein-binding disaccharides and disaccharide-binding serum proteins by affinity capillary electrophoresis.
The potential use of affinity capillary electrophoresis in a microscale search for mutually interacting substances in biological fluid is demonstrated. Some disaccharides, especially gentiobiose (Gen), derivatized with 1-phenyl-3-methyl-5-pyrazolone, caused peak retardation when electrophoresed in a neutral running buffer, containing human serum. Gen, the most significantly retarded disaccharide, was converted to its negatively charged bis-mercaptoethanesulfonate derivative (MerESGen), and a serum sample was analyzed in a neutral buffer containing the derivatized disaccharide. Two peaks, belonging to the beta-globulin fraction, were found to be remarkably retarded in the buffer containing MerES-Gen in a concentration-dependent way. These findings prove an interaction between disaccharides and serum proteins.
Faculty of Pharmaceutical Sciences, Kinki University, 3-4-1 Kowakae, 577-8502 Higashi-osaka, Japan.
Atsushi
A
Taga
Yuka
Y
Yamamoto
Rie
R
Maruyama
Susumu
S
Honda
D001609:Beta-Globulins / Q000737:chemistry / Q000302:isolation & purification; D001798:Blood Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002352:Carrier Proteins / Q000097:blood* / Q000302:isolation & purification*; D004187:Disaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D009281:Naphthalenes / Q000737:chemistry; D011485:Protein Binding
D001609:Beta-Globulins; D001798:Blood Proteins; D002352:Carrier Proteins; D004187:Disaccharides; D009281:Naphthalenes; C046465:8-amino-1,3,6-naphthalenetrisulfonic acid; C100052:gentiobiose