[Immunolocalization of laminin during reparative dentinogenesis].
OBJECTIVE
To investigate the immunolocalization of laminin in the process of dental pulp injury and repair.


METHODS
One cavity was prepared on the mesial surface of the first molars of Wistar rats. The animals were sacrificed in 3, 15, and 30 days of post-operation. After histological process, the paraffin sections were reacted with monoclonal antibodies against rat laminin using SABC method.


RESULTS
Immunolocalization demonstrated positive staining for laminin in the different stages of dental pulp repair. In 3 days group, no reparative dentin were observed. The positive staining was located in odontoblasts and in predentin. After 15 days, reparative dentin and odontoblasts were observed. The odontoblast like cells and dental pulp cells were stained positively. 30 days later, the stronger staining intensity was observed in odontoblast like cells, pulp cells and in reparative dentin. Immunological reaction was particularly located among the interface of reparative dentin and dental pulp.


CONCLUSIONS
The localization feature of laminin suggested that laminin may be a favored factor for the adhesion of dental pulp cell during reparative dentinogenesis.
School and Hospital of Stomatology, Wuhan University, China. zhichen@39.net
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