Effect of disruption of Staphylococcus aureus PBP4 gene on resistance to beta-lactam antibiotics.
Penicillin-binding proteins (PBPs) mediate susceptibility to beta-lactam antibiotics. PBP 4, although not essential for survival, has been associated with low-level resistance to beta-lactam antibiotics. To determine its contribution to survival of Staphylococcus aureus cells exposed to beta-lactams, the PBP 4 gene (pbp4) was disrupted and then complemented in the methicillin-susceptible strain RN4220 and the homogeneous methicillin-resistant strain COL. Depending on the antibiotic tested, the presence or absence of an intact pbp4 has no effect or only a modest effect on growth measured by population analysis. These data indicate that PBP 4 is a relatively unimportant target of beta-lactams not only in methicillin-susceptible but also methicillin-resistant S. aureus.
Division of Infectious Diseases, San Francisco General Hospital, Department of Medicine, University of California San Francisco, San Francisco, CA 94110, USA.
Yuki
Y
Katayama
Hong-Zhong
HZ
Zhang
Henry F
HF
Chambers
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D018274:Electroporation; D005798:Genes, Bacterial / Q000235:genetics; D005816:Genetic Complementation Test; D006602:Hexosyltransferases / Q000235:genetics*; D002267:Muramoylpentapeptide Carboxypeptidase / Q000235:genetics*; D016296:Mutagenesis; D009254:Nafcillin / Q000494:pharmacology; D046915:Penicillin-Binding Proteins; D010406:Penicillins / Q000494:pharmacology; D010458:Peptidyl Transferases / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013211:Staphylococcus aureus / Q000235:genetics* / Q000254:growth & development; D018440:beta-Lactam Resistance / Q000235:genetics*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D046915:Penicillin-Binding Proteins; D010406:Penicillins; D009254:Nafcillin; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase