cDNA representational difference analysis of ileal Peyer's patches in lambs after oral inoculation with scrapie.
cDNA representational difference analysis (RDA) was used to study gene expression profiles in the ileal Peyer's patch of a lamb 1 week after oral inoculation with the scrapie agent. Twenty-five differentially expressed cDNA fragments were identified and cloned. Sequence analysis indicated seven novel gene sequences. Other clones shared sequence homology with genes encoding ribosomal and mitochondrial proteins, the translation initiation factor EIF4GII and the bovine pancreatic thread protein. Reverse Northern was used to confirm the differential expression in another four lambs inoculated with scrapie and the tissue distribution of the novel genes was examined using Northern blot analysis.
Skretting
Grethe
G
Department of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary Science, P.O. Box 8146 Dep., N-0033 Oslo, Norway. grethe.skretting@veths.no
Espenes
Arild
A
Ulvund
Martha J
MJ
Olsaker
Ingrid
I
D000284:Administration, Oral; D000818:Animals; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D020869:Gene Expression Profiling; D007082:Ileum / Q000276:immunology*; D010581:Peyer's Patches / Q000378:metabolism*; D018620:PrPSc Proteins / Q000008:administration & dosage; D012333:RNA, Messenger / Q000378:metabolism; D012608:Scrapie / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D012756:Sheep
D018076:DNA, Complementary; D018620:PrPSc Proteins; D012333:RNA, Messenger