[Study on microtubulin of human rhabdomyosarcoma].
OBJECTIVE
To detect the expression of microtubules in human rhabdomyosarcoma.


METHODS
Expression of microtubulin-beta in 70 tissue slides from human rhabdomyosarcoma was detected by immunohistochemistry. Immunofluorescent staining was used to examine microtubules in PLA-802, A673 and RD under laser scanning confocal microscope.


RESULTS
Expression of microtubulin-beta in human rhabdomyosarcoma tissue was widely decreased. The low expression rate of microtubulin-beta in histological grade-I tumor was 52.38%, and that in histological grade-III tumor 85% (P < 0.05). The low expression rate of microtubulin-beta in 32 cases with metastasis and recurrence was 87.5%, and that in 38 cases without metastasis and recurrence 55.26%. Microtubules in the three cell lines showed different degrees of maldevelopment. The most severe degree was observed in PLA-802, and the less severe degree in RD (P < 0.05).


CONCLUSIONS
The microtubulin shows extensive low expression in human rhabdomyosarcoma. The decrease and hypogenesis of microtubules in human rhabdomyosarcoma have relationship with tumor's histological grade, malignant degree, metastasis, and recurrence.
Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, China.
Lu
L
Ye
Hong-ying
HY
Zhang
Hong
H
Bu
Guang-hua
GH
Yang
Jie
J
Zhang
Li-xin
LX
Guo
Shou-li
SL
Wang
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D011379:Prognosis; D018232:Rhabdomyosarcoma, Alveolar / Q000378:metabolism* / Q000473:pathology; D018233:Rhabdomyosarcoma, Embryonal / Q000378:metabolism* / Q000473:pathology; D012983:Soft Tissue Neoplasms / Q000378:metabolism / Q000473:pathology; D014407:Tumor Cells, Cultured
D008869:Microtubule-Associated Proteins