Relationship between serum testosterone and activities of testicular enzymes after continuous and intermittent training in male rats.
To gain more information on the effects of training types on testosterone secretion, the present study investigated the relationship between serum testosterone (ST) and the activities of oxydoreductive enzymes in Leydig cells to continuous and intermittent training regimes. Male rats swam with a load of 3.5 % body weight for 90 min in the continuous training group, and 15 min separated by a 7-min rest interval x 6 times in the intermittent training group, 6 days per week for 5 weeks. ST were measured immediately and 24 h after exercise, and the activities of SDH, LDH and G6PDH in Leydig cells were measured 24 h after exercise, following 5 weeks of training. It was found that ST declined following continuous (0.54 +/- 0.32 nmol/l) and intermittent (1.64 +/- 1.80 nmol/l) exercise compared to sedentary group (9.55 +/- 5.17 nmol/l). This diminishing effect on ST was still significant 24 h after continuous exercise (5.96 +/- 2.79 nmol/l), not after intermittent exercise (7.41 +/- 4.77 nmol/l). The activities of SDH and LDH increased, whereas G6PDH decreased in Leydig cells, after both continuous and intermittent training. SDH and G6PDH showed the high activities in the intermittent training group relative to continuous training group. These differences in the activities of SDH and G6PDH might be considered as the possible causes for ST responses to training types.
Hu
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Y
Section of Exercise Biochemistry, Department of Exercise and Human Sciences, Beijing University of Physical Education, Beijing, China. huyang@bsu.edu.cn
Asano
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K
Kim
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S
Nagata
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H
D000818:Animals; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013737:Testis / Q000201:enzymology*; D013739:Testosterone / Q000097:blood*
D013739:Testosterone; D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D013385:Succinate Dehydrogenase