Osteoclastogenesis on tissue-engineered bone.
Bone remodeling plays an important role in bone function. To date, bone tissue-engineering research has focused primarily on bone formation from osteoblasts. This study demonstrates that osteoclastogenesis can occur on a mineralized polymer scaffold. Porcine bone marrow-derived mesenchymal stem cells (pMSCs) and hematopoietic cells were isolated from the bone marrow of Yucatan minipigs (n = 3) and cultured separately. pMSCs were differentiated into osteoblasts, seeded on porous poly(D,L-lactic-co-glycolic acid) foams, and cultured in a rotating oxygen-permeable bioreactor system. Once the cell-polymer constructs had started to mineralize, the hematopoietic cells were added and cocultured to include osteoclastogenesis. The cultured constructs were evaluated by histochemical and microscopic examination. Our results show that osteoblasts and osteoclasts were successfully differentiated from bone marrow on the scaffolds. This is the first demonstration of osteoclast formation on mineralized polymer surfaces.
Department of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA.
Keisuke
K
Nakagawa
Harutsugi
H
Abukawa
Michael Y
MY
Shin
Hidetomi
H
Terai
Maria J
MJ
Troulis
Joseph P
JP
Vacanti
D000818:Animals; D018786:Bone Substitutes; D002454:Cell Differentiation / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology; D059630:Mesenchymal Stem Cells; D010006:Osteoblasts / Q000502:physiology*; D010010:Osteoclasts / Q000502:physiology*; D019736:Prostheses and Implants; D013552:Swine; D023822:Tissue Engineering
D018786:Bone Substitutes