Rapid and simple high-performance liquid chromatographic determination of felbamate in serum.
An isocratic high-performance liquid chromatographic method for the quantitation of felbamate in serum is presented. The method is based on protein precipitation with acetonitrile and reversed-phase chromatography with spectrophotometric detection at 210 nm. The separation was performed on a Nova-Pak C18 column and the mobile phase consisted of acetonitrile-water (1:4, v/v). Phenobarbital was applied as an internal standard. The assay was linear over the concentration range 0.5-200.0 microg/ml (r = 0.9999). Intra-day and inter-day precision expressed by relative standard deviation is less than 3%. The percentage of recovery was 98.63 +/- 2.47. The method is suitable for drug level monitoring and for pharmacokinetic studies.
Department of Medicinal Chemistry, Faculty of Pharmacy, Medical University, 6 Chodźki Str., 20-093 Lublin, Poland.
Beata
B
Paw
Genowefa
G
Misztal
Robert
R
Skibiński
D000927:Anticonvulsants / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000078328:Felbamate; D006801:Humans; D048448:Phenylcarbamates; D011409:Propylene Glycols / Q000097:blood*; D012680:Sensitivity and Specificity
D000927:Anticonvulsants; D048448:Phenylcarbamates; D011409:Propylene Glycols; D000078328:Felbamate