In ovo electroporation of avian somites.
In ovo electroporation is a well-established method of gene transfer into neural and mesenchymal tissue in chick embryos. Electroporation of somites, however, has been hampered by low efficiency due to technical difficulties. Here, we present a powerful technique to electroporate avian somites and subpopulations of somitic cells at high efficiency in ovo. We demonstrate specific targeting of distinct somitic compartments and their derivatives using single or combinations of plasmid expression vectors. This technique opens new perspectives to investigate the morphologic and genetic basis of somite development.
Scaal
Martin
M
Laboratoire de Génétique et de Physiologie du Développement, Developmental Biology Institute of Marseille (IBDM), CNRS UMR 654, University Aix-Marseille II, Campus de Luminy, Marseille, France.
Gros
Jerome
J
Lesbros
Cynthia
C
Marcelle
Christophe
C
D000818:Animals; D001717:Birds; D019070:Cell Lineage; D002465:Cell Movement; D002642:Chick Embryo; D018274:Electroporation / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D015203:Reproducibility of Results; D019170:Somites / Q000378:metabolism*; D019076:Transgenes
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins