Splenic ellipsoids: an early target for deposition of AA amyloid induced in mink.
The spleen is the primary target for spontaneous as well as experimental AA amyloidosis in animals such as mice and mink, and is therefore a valuable organ for study of the initial phases of amyloid fibrillogenesis and deposition. We have investigated splenic amyloid AA deposits induced in the mink, and we demonstrate a novel target for AA, namely the splenic ellipsoids. We show presence of amyloid P component (AP), glycosaminoglycans (GAGs) and apolipoprotein E (apoE), all well-known common elements of amyloid, co-localizing with AA. In addition, apolipoprotein AI (apoAI) was seen co-localized to the AA deposits in the ellipsoids. We hypothesize that the ellipsoids may be important splenic structures for initial AA formation. The apoAI in the ellipsoids could displace SAA from acute phase HDL at this site, thereby making SAA available for amyloid formation and deposition.
Wien
Tale N
TN
Department of Rheumatology/Institute of Immunology, Rikshospitalet, University of Oslo, 0027 Oslo, Norway. t.n.wien@klinmed.uio.no
Sørby
Randi
R
Espenes
Arild
A
Gunnes
Gjermund
G
Nordstoga
Knut
K
Landsverk
Thor
T
Husby
Gunnar
G
D000686:Amyloidosis / Q000378:metabolism*; D000818:Animals; D016632:Apolipoprotein A-I / Q000032:analysis; D001057:Apolipoproteins E / Q000032:analysis; D004926:Escherichia coli; D006025:Glycosaminoglycans / Q000032:analysis; D007124:Immunoenzyme Techniques; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008075:Lipoproteins, HDL / Q000378:metabolism; D008907:Mink; D000685:Serum Amyloid A Protein / Q000032:analysis / Q000378:metabolism*; D000683:Serum Amyloid P-Component / Q000032:analysis; D013154:Spleen / Q000378:metabolism*
D016632:Apolipoprotein A-I; D001057:Apolipoproteins E; D006025:Glycosaminoglycans; D008070:Lipopolysaccharides; D008075:Lipoproteins, HDL; D000685:Serum Amyloid A Protein; D000683:Serum Amyloid P-Component