Multicolor spectral karyotyping of rat chromosomes.
Rat and mouse have become important animal models to study various human diseases such as cancer. Cytogenetic analysis of the respective karyotypes is frequently required to investigate the causative genetic defects and especially neoplastic cells often show complex chromosome aberrations and many different marker chromosomes. However, structural homogeneity of the chromosomes in these species as well as less pronounced differences in banding patterns make it difficult to assign genetic abnormalities to certain chromosomes by conventional banding techniques. Here we report for the first time the successful application of multicolor spectral karyotyping (SKY) to rat chromosomes, which allows unequivocal identification of all rat chromosomes with the exception of chromosomes 13 and 14 in different colors, thus enabling the elucidation of even complex rearrangements in the rat karyotype. Flow-sorted chromosome specific painting probes for all 22 rat chromosomes (20 autosomes, X, and Y) were combinatorially labeled by a set of five different fluorochromes and hybridized in situ to metaphase spreads of a healthy rat, to diakineses from testicular material, and to cells from a rat FAO hepatoma cell line. Measuring the complete spectrum at each image point by using the SpectraCube((R)) spectral imaging system and respective computer software allowed identification of the individual rat chromosomes by their specific emission spectra. Classification algorithms in the analysis software can then display the rat chromosomes in specific pseudo-colors and automatically order them in a karyotype table. After its successful application to human and mouse chromosomes, spectral karyotyping of rat chromosomes now also allows cytogenetic screening of the complete rat genome by a single hybridization.
Department of Human Genetics, University of Würzburg, Biozentrum, Würzburg (Germany).
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A
Buwe
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C
Steinlein
M R
MR
Koehler
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I
Bar-Am
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Katzin
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Schmid
D000818:Animals; D045744:Cell Line, Tumor; D020223:Chromosome Painting / Q000379:methods*; D033481:Chromosomes, Mammalian / Q000648:ultrastructure; D003116:Color; D007621:Karyotyping / Q000379:methods*; D008297:Male; D008677:Metaphase; D051381:Rats / Q000235:genetics*