The inhibitory effects of 12 medicinal plants and their component compounds on lipid peroxidation.
The antioxidative activities of 12 medicinal plants and the compounds isolated from them were investigated using the thiocyanate method to evaluate inhibitory effects on lipid peroxidation in the linoleic acid system. The peroxide levels gradually increased during incubation in the presence of linoleic acid over 3 days, and most of the plants inhibited lipid peroxidation. In particular, of the plants tested, Cudrania tricuspidata, Zanthoxylum piperitum, Houttuynia cordata and Ulmus parvifolia reduced lipid peroxidation more effectively as lipid peroxidation progressed, resulting in inhibition of about 80% relative to the control value by the 3rd day of incubation. In addition, the polyphenols isolated from the plants also showed marked and dose-dependent inhibitory effects on lipid peroxidation. The compounds with the strongest activities were 3,4-dihydroxylbenzoic acid, quercetin, the quercetin glycosides quercetin-3-O-beta-D-galactoside, quercetin-3-O-alpha-L-rhamnoside, quercetin-3-O-beta-D-glucoside and quercetin-3-O-rutinose, catechin, gallic acid, methyl gallate and rosamultin isolated from Zanthoxylum piperitum, Houttuynia cordata, Rosa rugosa and Cedrela sinensis. Moreover, quercetin glycosides showed stronger activity than quercetin, suggesting that glycosylation increases the antioxidative activity of quercetin. Our results indicate that the medicinal plants and their polyphenols show promise as therapeutic agents for various disorders involving free radical reactions.
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan.
Eun Ju
EJ
Cho
Takako
T
Yokozawa
Dong Young
DY
Rhyu
Hyun Young
HY
Kim
Naotoshi
N
Shibahara
Jong Cheol
JC
Park
D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000378:metabolism; D019787:Linoleic Acid / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D000432:Methanol; D018384:Oxidative Stress / Q000187:drug effects; D010944:Plants / Q000737:chemistry / Q000378:metabolism*; D013861:Thiocyanates
D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D013861:Thiocyanates; D019787:Linoleic Acid; C031760:thiocyanate; D000432:Methanol