[Determining conjugated bile acids by reverse HPLC and analyzing the conjugated bile acids in sera of patients with hepatocirrhosis].
OBJECTIVE
To investigate the conjugated bile acids in sera of patients with hepatocirrhosis by the determination of conjugated bile acids using reverse HPLC(high performance liquid chromatography).


METHODS
Reverse HPLC was used to determine the conjugated bile acids, to evaluate the precision, accuracy, sensitivity and linear limitation of the method, and to separate and determine six conjugated bile acids in the sera of hepatocirrhotic patients. A comparison with controls was performed.


RESULTS
The limitation of determination was 3.125 mumol/L. The coefficients of variation were less than 10% within day and less than 15% day to day for the concentration (3.5-100 mumol/L) of bile acids, and the recoveries were 93.3%-106.1% except TLCA. The concentrations of conjugated bile acids in sera of patients with hepatocirrhosis were significantly higher than those of the control group (P < 0.05).


CONCLUSIONS
The method can meet the need of the determination of conjugated bile acids in serum. The concentrations of conjugated bile acids in sera of patients with hepatocirrhosis are of use for clinical diagnosis and treatment.
Laboratory Department, West China Hospital, Sichuan University, Chengdu 610041, China.
Gui-xing
GX
Li
Ping
P
Li
Bing
B
Gao
D001647:Bile Acids and Salts / Q000097:blood*; D015415:Biomarkers; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood*
D001647:Bile Acids and Salts; D015415:Biomarkers