Silencing SARS-CoV Spike protein expression in cultured cells by RNA interference.
The severe acute respiratory syndrome (SARS) has been one of the most epidemic diseases threatening human health all over the world. Based on clinical studies, SARS-CoV (the SARS-associated coronavirus), a novel coronavirus, is reported as the pathogen responsible for the disease. To date, no effective and specific therapeutic method can be used to treat patients suffering from SARS-CoV infection. RNA interference (RNAi) is a process by which the introduced small interfering RNA (siRNA) could cause the degradation of mRNA with identical sequence specificity. The RNAi methodology has been used as a tool to silence genes in cultured cells and in animals. Recently, this technique was employed in anti-virus infections in human immunodeficiency virus and hepatitis C/B virus. In this study, RNAi technology has been applied to explore the possibility for prevention of SARS-CoV infection. We constructed specific siRNAs targeting the S gene in SARS-CoV. We demonstrated that the siRNAs could effectively and specifically inhibit gene expression of Spike protein in SARS-CoV-infected cells. Our study provided evidence that RNAi could be a tool for inhibition of SARS-CoV.
Zhang
Yuanjiang
Y
Institute of Biomedicine, Tsinghua University, Beijing, PR China.
Li
Tieshi
T
Fu
Ling
L
Yu
Changming
C
Li
Yinghua
Y
Xu
Xialian
X
Wang
Yinyin
Y
Ning
Hongxiu
H
Zhang
Shuping
S
Chen
Wei
W
Babiuk
Lorne A
LA
Chang
Zhijie
Z
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D002522:Chlorocebus aethiops; D017934:Coronavirus / Q000235:genetics*; D015870:Gene Expression; D020868:Gene Silencing; D005822:Genetic Vectors; D006389:Hemagglutinins, Viral / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000196:embryology; D035683:MicroRNAs / Q000235:genetics; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D012367:RNA, Viral / Q000302:isolation & purification; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics*; D045169:Severe Acute Respiratory Syndrome / Q000517:prevention & control; D014709:Vero Cells; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism*
D006389:Hemagglutinins, Viral; D035683:MicroRNAs; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012367:RNA, Viral; D015678:Viral Structural Proteins