Detection of Cryptosporidium parvum in soil extracts.
Epifluorescent microscopy and flow cytometry were used in different combinations with fluorescein isothiocyanate-labeled immunoglobulins M and G3 to estimate the numbers of Cryptosporidium parvum oocysts in soil extracts containing 10 to 10,017 oocysts/ml. No combination had a systematic effect on accuracy or precision. Background debris may have produced overestimates at low oocyst concentrations when flow cytometry was used.
Department of Natural Resources and Environmental Sciences, University of Nevada, Reno, Nevada 89557, USA. mwalker@equinox.unr.edu
Mark
M
Walker
Douglas
D
Redelman
D000818:Animals; D000913:Antibodies, Protozoan; D016785:Cryptosporidium parvum / Q000276:immunology / Q000302:isolation & purification*; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D008856:Microscopy, Fluorescence; D033661:Oocysts / Q000302:isolation & purification; D012987:Soil / Q000469:parasitology*
D000913:Antibodies, Protozoan; D005456:Fluorescent Dyes; D012987:Soil; D016650:Fluorescein-5-isothiocyanate