[Experimental research of PDE5 activity in corpora cavernosa of ganyu qizhi rat model].
OBJECTIVE
To study the PDE5 activity in corpora cavernosa of the Ganyu Qizhi model penis and the effect of the Chinese herbal medicine Shugan Liqi Huoxue (SLH) ointment on it.


METHODS
Non-injury stress stimulus method similar to human spirit stress was used to extablish the Ganyu Qizhi animal(rat) model, and the PDE5 activity in corpora cavernosa of the rat penis was measured by the method of immunohistochemistry and computer image analysis.


RESULTS
The PDE5 activity in corpora cavernosa of the high-dosage SLH group was significantly different from that of the model group (P < 0.01).


CONCLUSIONS
Ganyu Qizhi may increase the PDE5 activity in corpora cavernosa of the penis while SLH can reduce such activity.
Department of Andrology, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 610072, China. changdg@mail.sc.cnicfo.net
Degui
D
Chang
Zhengyi
Z
Wang
Bide
B
Zhu
Jiuyuan
J
Wang
Shuwu
S
Zhang
Zhiping
Z
Hu
D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000378:metabolism*; D000818:Animals; D054706:Cyclic Nucleotide Phosphodiesterases, Type 5; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007172:Erectile Dysfunction / Q000209:etiology; D008297:Male; D008516:Medicine, Chinese Traditional; D023421:Models, Animal; D010413:Penis / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D054706:Cyclic Nucleotide Phosphodiesterases, Type 5; C517381:PDE5A protein, human; C517384:Pde5a protein, rat