A membrane-anchored protein kinase involved in Brassica self-incompatibility signaling.
Self-incompatibility (SI) response in Brassica is initiated by haplotype-specific interactions between the pollen-borne ligand S locus protein 11/SCR and its stigmatic S receptor kinase, SRK. This binding induces autophosphorylation of SRK, which is then thought to trigger a signaling cascade that leads to self-pollen rejection. A recessive mutation of the modifier (m) gene eliminates the SI response in stigma. Positional cloning of M has revealed that it encodes a membrane-anchored cytoplasmic serine/threonine protein kinase, designated M locus protein kinase (MLPK). Transient expression of MLPK restores the ability of mm papilla cells to reject self-pollen, suggesting that MLPK is a positive mediator of Brassica SI signaling.
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0101, Japan.
Kohji
K
Murase
Hiroshi
H
Shiba
Megumi
M
Iwano
Fang-Sik
FS
Che
Masao
M
Watanabe
Akira
A
Isogai
Seiji
S
Takayama
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D029741:Brassica rapa / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D002462:Cell Membrane / Q000201:enzymology*; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000201:enzymology; D035264:Flowers / Q000201:enzymology / Q000502:physiology*; D017343:Genes, Plant; D006239:Haplotypes; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D016366:Open Reading Frames; D010766:Phosphorylation; D020161:Physical Chromosome Mapping; D010940:Plant Proteins; D011058:Pollen / Q000502:physiology; D011494:Protein Kinases / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015398:Signal Transduction
D008565:Membrane Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; C079832:S-receptor kinase