[The development and ultrastructure study of acellular submandibular gland scaffold].
OBJECTIVE
To develop nature acellular submandibular gland scaffold materials and observe its ultrastructures.


METHODS
The fresh submandibular glands of SD rats were cell-extracted by chemical detergent and observed with transmission and scanning electron microscope.


RESULTS
Submandibular gland cells were dissolved and destroyed under transmission electron microscope. Normal submandibular gland cells were ball, but the shape of cells disappeared after cell-extraction under scanning electron microscope. Collagen fibers were arranged as net structure.


CONCLUSIONS
Submandibular gland cells disappeared and the arrangement of collagen fibres were normal by Triton X-100 cell-extraction.
Department of Oral and Maxillofacial Surgery, First Clinical Hospital of China Medical University, Shenyang 110001, China.
Chun-fu
CF
Deng
Yue
Y
Liu
Xue-xin
XX
Tan
Qing
Q
Zhou
Shao-hui
SH
Huang
Yu-xin
YX
Wang
Tao
T
Xu
D000818:Animals; D005260:Female; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013363:Submandibular Gland / Q000648:ultrastructure*; D023822:Tissue Engineering