Real-time reverse transcriptase PCR assay for detection of human metapneumoviruses from all known genetic lineages.
The discovery of human metapneumovirus and its implications for respiratory tract disease have emphasized the need for a sensitive, specific, and rapid assay to detect this virus in a clinical setting. It recently became clear that human metapneumovirus can be grouped into at least four genetic lineages. Previously described assays for the detection of human metapneumovirus were developed by using limited sequence information and failed to detect viruses from all four genetic lineages with comparable sensitivities. Here we describe the development and evaluation of a real-time reverse transcriptase PCR assay that detects human metapneumovirus from the four known genetic lineages with equal specificities and sensitivities.
Maertzdorf
Jeroen
J
Vironovative BV., Rotterdam, The Netherlands.
Wang
Chiaoyin K
CK
Brown
Jennifer B
JB
Quinto
Joseph D
JD
Chu
Marla
M
de Graaf
Miranda
M
van den Hoogen
Bernadette G
BG
Spaete
Richard
R
Osterhaus
Albert D M E
AD
Fouchier
Ron A M
RA
D001483:Base Sequence; D017931:DNA Primers; D015342:DNA Probes; D006801:Humans; D029121:Metapneumovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D009305:Nasopharynx / Q000821:virology; D018184:Paramyxoviridae Infections; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D015342:DNA Probes