A new sequenced allelic ladder marker for D1S80 typing.
A sequenced allelic ladder marker that contains 32 alleles consisting of alleles 13-44 was developed for D1S80 ( MCT118) typing. Each allele, after amplification with D1S80 primers, was cloned to the pT7Blue T-Vector. The plasmid DNAs of these clones were mixed and amplified with D1S80 primers to construct the allelic ladder marker. All the alleles prepared in this study were sequenced in both directions, using two types of forward primers and one reverse primer. The sequencing results revealed that all the alleles in our allelic ladder marker had the correct length corresponding to the allele number. This marker can be used for more effectively typing D1S80.
Fujii
Koji
K
Fourth Biological Section, National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan. fujii@nrips.go.jp.
Sekiguchi
Kazumasa
K
Third Biological Section, National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan.
Shimizu
Kenshi
K
Forensic Science Laboratory, Miyazaki Prefectural Police Headquarters, 1-8-28 Asahi, Miyazaki 880-8509, Japan.
Kasai
Kentaro
K
Fourth Biological Section, National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan.
D000483:Alleles; D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes; D005819:Genetic Markers; D006579:Heterozygote; D006801:Humans; D008969:Molecular Sequence Data
D017931:DNA Primers; D015342:DNA Probes; D005819:Genetic Markers; D004247:DNA