Sub-second isoelectric focusing in free flow using a microfluidic device.
Using a microfabricated chip with a bed volume of 0.2 microL we demonstrate the validity of the scaling laws for molecular mass transport of isoelectric focusing (IEF) in free flow. Nano- or microlitre sample volumes can be concentrated within 430 ms by a factor of up to 400. These very fast performances make the chip applicable to proteomic analysis and for continuous monitoring of biochemical processes.
Xu
Yi
Y
Imperial College London, Chemistry Department, Exhibition Road, London, UK SW7 2AZ.
Zhang
Chao-Xuan
CX
Janasek
Dirk
D
Manz
Andreas
A
D000803:Angiotensin I / Q000032:analysis; D000804:Angiotensin II / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D007525:Isoelectric Focusing / Q000295:instrumentation*; D008838:Microcomputers; D036103:Nanotechnology; D010455:Peptides / Q000737:chemistry
D010455:Peptides; D000804:Angiotensin II; D007334:Insulin-Like Growth Factor I; D000803:Angiotensin I