Protective effects of quercetin and its metabolites on H2O2-induced chromosomal damage to WIL2-NS cells.
We investigated chromosomal damage caused by a typical flavonoid, quercetin, and its two conjugates, quercetin-3-O-sulfate and isorhamnetin, and their protective effects against chromosomal damage induced by H2O2. The chromosomal damage was detected by the cytokinesis-block micronucleus (CBMN) assay using a lymphoblastoid cell line, WIL2-NS. We found that quercetin itself induced chromosomal damage at 10 microM, but quercetin-3-O-sulfate and isorhamnetin did not induce damage up to 30 microM. In the medium used for the CBMN assay, quercetin (at 100 microM) generated a high concentration of H2O2, but the two conjugates did not at the same concentration. On the other hand, pretreatment with quercetin (at 1 microM), quercetin-3-O-sulfate (at 10 microM), and isorhamnetin (at 5 microM) prevented H2O2-induced chromosomal damage to WIL2-NS cells. These findings suggest that the induction and prevention of H2O2-induced chromosomal damage are different between quercetin and its metabolites.
Hokkaido Institute of Public Health, Sapporo, Hokkaido, Japan. saitoak@iph.pref.hokkaido.jp
Akiko
A
Saito
Ayako
A
Sugisawa
Keizo
K
Umegaki
Hiroyuki
H
Sunagawa
D000975:Antioxidants; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D019457:Chromosome Breakage; D003470:Culture Media; D005419:Flavonoids / Q000494:pharmacology; D044948:Flavonols / Q000494:pharmacology / Q000633:toxicity; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000633:toxicity*; D015162:Micronucleus Tests; D016877:Oxidants / Q000633:toxicity*; D011794:Quercetin / Q000494:pharmacology* / Q000633:toxicity / Q000031:analogs & derivatives
D000975:Antioxidants; D003470:Culture Media; D005419:Flavonoids; D044948:Flavonols; D016877:Oxidants; C047368:3-methylquercetin; D011794:Quercetin; D006861:Hydrogen Peroxide