Whole blood and mononuclear cell glutathione response to dietary whey protein supplementation in sedentary and trained male human subjects.
Sedentary male subjects (n=9) on a controlled diet were fed two doses (0.8 or 1.6 g/kg body mass) of a whey protein isolate (WPI), in addition to an isocaloric placebo; blood samples were drawn over a 4-h period and glutathione concentration determined. There was no effect of the supplementation at either level over the 4-h sampling period. The effects of a WPI supplemented diet on glutathione concentrations in whole blood as well as peripheral mononuclear cell populations were also investigated over a 6-week period in male subjects (n=18) involved in arduous aerobic training; blood was collected prior to and following a 40 km simulated cycling trial. The aerobic training period resulted in significantly lower glutathione concentrations in whole blood, an effect that was mitigated by WPI supplementation. A significant increase in mononuclear cell glutathione was also observed in subjects receiving the WPI supplement following the 40 km simulated cycling trial.
Faculty of Agriculture, Food and Nutritional Sciences, University of Alberta, Edmonton, Alberta, Canada.
N
N
Middleton
P
P
Jelen
G
G
Bell
D000328:Adult; D000596:Amino Acids / Q000032:analysis; D001642:Bicycling / Q000502:physiology; D004044:Dietary Proteins / Q000494:pharmacology*; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D015444:Exercise / Q000502:physiology; D005978:Glutathione / Q000097:blood*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008894:Milk Proteins / Q000494:pharmacology*; D000067816:Whey Proteins
D000596:Amino Acids; D004044:Dietary Proteins; D008894:Milk Proteins; D000067816:Whey Proteins; D005978:Glutathione