Selective inhibition of the growth of cancer cells by diterpenes selected with embryonic cells of Xenopus.
We used Xenopus embryo cells with a cell cycle of 20-30 min to detect inhibitory effects on cell proliferation. Inhibition of proliferation was observed when isolated embryonic cells were incubated for 16 h in a simple salt solution containing the well-known anticancer drugs 5-fluorouracil and adriamycin. In addition, three diterpene compounds isolated from the anticancer herbal medicine kansui: kansuinin B, 20-OD-ingenol Z, and 20-OD-ingenol E specifically inhibited the proliferation of isolated embryonic cells. The inhibitory compounds selected using the embryonic cells also inhibited proliferation of certain mammalian cell types.
Miyata
Shohei
S
Department of Chemistry, College of Humanities and Sciences, Nihon University, Sakurajosui, Setagaya-ku, Tokyo 156-8550, Japan. miyata@chs.nihon-u.ac.jp
Wang
Li-Yan
LY
Wang
Nai-Li
NL
Yao
Xin-Sheng
XS
Kitanaka
Susumu
S
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000187:drug effects*; D009369:Neoplasms / Q000188:drug therapy*; D014407:Tumor Cells, Cultured; D014981:Xenopus
D000970:Antineoplastic Agents; D004224:Diterpenes