Characterization of acetylcholinesterases, and their genes, from the hemipteran species Myzus persicae (Sulzer), Aphis gossypii (Glover), Bemisia tabaci (Gennadius) and Trialeurodes vaporariorum (Westwood).
Gene sequences encoding putative acetylcholinesterases have been reported for four hemipteran insect species. Although acetylcholinesterase insensitivity occurs in insecticide-resistant populations of each of these species, no mutations were detected in the gene sequences from the resistant insects. This, coupled with a series of experiments using novel reversible inhibitors to compare the biochemical characteristics of acetylcholinesterase from a range of insect species, showed that the cloned cDNA fragments are unlikely to encode the hemipteran synaptic acetylcholinesterases, and there is likely to be a second ace locus.
Rothamsted Research, Harpenden, Herts, UK.
N
N
Javed
R
R
Viner
M S
MS
Williamson
L M
LM
Field
A L
AL
Devonshire
G D
GD
Moores
D000110:Acetylcholinesterase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D002219:Carbamates; D016000:Cluster Analysis; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D006430:Hemiptera / Q000235:genetics*; D006843:Hydrocarbons, Chlorinated; D020128:Inhibitory Concentration 50; D007306:Insecticides / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D002219:Carbamates; D017931:DNA Primers; D018076:DNA, Complementary; D006843:Hydrocarbons, Chlorinated; D007306:Insecticides; D000110:Acetylcholinesterase