Comparison of three different phosphorescent methodologies in solution for the analysis of naphazoline in pharmaceutical preparations.
We present results from a comparative study of three proposed phosphorimetric methods for determination of naphazoline (NPZ) in solution. The first method is based on use of micelles to stabilize phosphorescence signals in solutions at room temperature (MS-RTP). The second is based on the use of a heavy atom salt and sodium sulfite as an oxygen scavenger to obtain room-temperature phosphorescence (HAI-RTP) in solution. The last method employs an optical sensor for NPZ based on the phosphorescent properties of the analyte on a solid sensor phase. The aim of this work was to compare time consumption, simplicity, sensitivity, selectivity, detection, and quantification limits for use of these three phosphorimetric methods to determine naphazoline in pharmaceutical preparations. The most simple, sensitive, and reproducible of the three methods for naphazoline analysis is the HAI-RTP method. Detection limits are 4.9, 1.7, and 9.4 ng mL(-1), respectively, for the MS-RTP, HAI-RTP, and optosensor methods.
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Campus Fuentenueva s/n, 18071 Granada, Spain.
Beatriz Cañabate
BC
Díaz
Silvia Casado
SC
Terrones
Antonio Segura
AS
Carretero
José Manuel Costa
JM
Fernández
Alberto Fernández
AF
Gutiérrez
D008163:Luminescent Measurements; D008722:Methods; D009278:Naphazoline / Q000032:analysis*; D009883:Ophthalmic Solutions / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D012996:Solutions; D013050:Spectrometry, Fluorescence
D009883:Ophthalmic Solutions; D004364:Pharmaceutical Preparations; D012996:Solutions; D009278:Naphazoline