A mutation in the latency-related gene of bovine herpesvirus 1 inhibits protein expression from open reading frame 2 and an adjacent reading frame during productive infection.
The latency-related (LR) gene of bovine herpesvirus 1 (BHV-1) is abundantly expressed during latency. A mutant BHV-1 strain that contains three stop codons at the 5' terminus of the LR gene (LR mutant) does not reactivate from latency. This study demonstrates that the LR mutant does not express open reading frame 2 or an adjacent reading frame that lacks an initiating ATG (reading frame C). Since the LR mutant and wild-type BHV-1 express similar levels of LR RNA, we conclude that LR protein expression plays an important role in regulating the latency reactivation cycle in cattle.
Jiang
Yunquan
Y
Department of Veterinary and Biomedical Sciences, Nebraska Center for Virology, University of Nebraska, Lincoln, Nebraska 68583-0905, Spain.
Inman
Melissa
M
Zhang
Yange
Y
Posadas
Nuria Alemañ
NA
Jones
Clinton
C
D000818:Animals; D002417:Cattle; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D007242:Herpesvirus 1, Bovine / Q000472:pathogenicity*; D009154:Mutation; D016366:Open Reading Frames / Q000235:genetics / Q000502:physiology*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014775:Virus Activation; D017735:Virus Latency / Q000235:genetics*
D014764:Viral Proteins; C401822:latency-related protein, Bovine herpesvirus 1