Tissue engineering of fish skin: behavior of fish cells on poly(ethylene glycol terephthalate)/poly(butylene terephthalate) copolymers in relation to the composition of the polymer substrate as an initial step in constructing a robotic/living tissue hybrid.
This study presents the development of a biosynthetic fish skin to be used on aquatic robots that can emulate fish. Smoothness of the external surface is desired in improving high propulsive efficiency and maneuvering agility of autonomous underwater vehicles such as the RoboTuna (Triantafyllou, M., and Triantafyllou, G. Sci. Am. 272, 64, 1995). An initial step was to determine the seeding density and select a polymer for the scaffolds. The attachment and proliferation of chinook salmon embryo (CHSE-214) and brown bullhead (BB) cells were studied on different compositions of a poly(ethylene glycol terephthalate) (PEGT) and poly(butylene terephthalate) (PBT) copolymer (Polyactive). Polymer films were used, cast of three different compositions of PEGT/PBT (weight ratios of 55/45, 60/40, and 70/30) and two different molecular masses of PEGT (300 and 1000 Da). When a 55 wt% and a 300-Da molecular mass form of PEGT was used, maximum attachment and proliferation of CHSE-214 and BB cells were achieved. Histological studies and immunostaining indicate the presence of collagen and cytokeratins in the extracellular matrix formed after 14 days of culture. Porous scaffolds of PEGT/PBT copolymers were also used for three-dimensional tissue engineering of fish skin, using BB cells. Overall, our results indicate that fish cells can attach, proliferate, and express fish skin components on dense and porous Polyactive scaffolds.
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139-4307, USA.
Roxane
R
Pouliot
Rosa
R
Azhari
Hala F
HF
Qanadilo
Tahir A
TA
Mahmood
Michael S
MS
Triantafyllou
Robert
R
Langer
D000818:Animals; D002448:Cell Adhesion; D046508:Culture Techniques; D005399:Fishes; D010590:Phalloidine; D011091:Polyesters; D011093:Polyethylene Terephthalates; D011137:Polystyrenes; D012371:Robotics; D012867:Skin / Q000166:cytology; D017768:Skin, Artificial; D013194:Staining and Labeling
D011091:Polyesters; D011093:Polyethylene Terephthalates; D011137:Polystyrenes; D010590:Phalloidine; C041733:poly(1,4-butylene terephthalate)