[Preliminary analysis on China Agricultural University chicken resource population based on genomic scanning].
Combining the technique of multiplex-PCR and the fluorescent semi-automated detection, a large-scale genome scanning was performed for 440 chickens, which was derived from China Agricultural University chicken resource families, within three generations. Fifty-five microsatellite markers were analyzed for this study. Those 55 microsatellite loci accorded with the characters of Mendelian co-inheritance. The heterozygosities ranged from zero to 0.89, with 72% of loci having a heterozygosity of more than 0.60. The polymorphism information content (PIC) ranged from 0 to 0.85, in which 70% of those loci had a PIC of more than 0.50 but their distribution varied in line A and line C. The allele frequency was significantly different between line A and line C at most loci (P < 0.01). At the same time, gene accordance inclination was found in line C. The Nei population resemble coefficient and standard genetic distance were 0.1002 and 0.8928.
State Key Laboratory for Agro-biotechnology, China Agricultural University, Beijing 100094, China.
Xiao-Xiang
XX
Hu
Yin-Hua
YH
Huang
Yu
Y
Gao
Zhi-Qiang
ZQ
Du
Ji-Dong
JD
Feng
Wei-Zhuo
WZ
Xu
Xue-Mei
XM
Deng
Ying
Y
Zhang
Bao-Liang
BL
Fan
Zhi-Hui
ZH
Zhao
Zhao-Liang
ZL
Liu
Ning
N
Yang
Ning-Yi
NY
Jin
Chang-Xin
CX
Wu
Ning
N
Li
D000818:Animals; D002645:Chickens / Q000235:genetics*; D016678:Genome; D005838:Genotype; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic