Effect of high pressure gaseous carbon dioxide on the germination of bacterial spores.
Effect of high pressure gaseous carbon dioxide treatment (HGCT) at 6.5 MPa, 35 degrees C on the germination of bacterial spores was investigated. Germination of bacterial spores was estimated by the decrease of heat tolerance. Approximately, 40% of Bacillus coagulans and 70% of Bacillus licheniformis were germinated by HGCT for 120 min at 35 degrees C, respectively. Germination was confirmed by phase contrast microscopy. The effect of hydrostatic pressure treatment (HPT) at 6.5 MPa, 35 degrees C on the germination of B. coagulans and B. licheniformis spores were also investigated. Spores did not germinate by HPT alone at 6.5 MPa for 120 min.
Laboratory of Food Microbiology, Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, 1866, Kameino, Fujisawa 252-8510, Japan. furukawa@brs.nihon-u.ac.jp
S
S
Furukawa
T
T
Watanabe
T
T
Tai
J
J
Hirata
N
N
Narisawa
T
T
Kawarai
H
H
Ogihara
M
M
Yamasaki
D001407:Bacillus / Q000254:growth & development / Q000502:physiology*; D002245:Carbon Dioxide / Q000494:pharmacology*; D005511:Food Handling / Q000379:methods; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods; D006358:Hot Temperature; D006874:Hydrostatic Pressure; D008858:Microscopy, Phase-Contrast; D013171:Spores, Bacterial / Q000187:drug effects* / Q000254:growth & development; D013997:Time Factors
D002245:Carbon Dioxide