[Effects of 17 beta-estradiol on the gene expression of shear stressed endothelial cells].
OBJECTIVE
To determine the protective effect of 17 beta-estradiol on endothelial cells under shear stress.


METHODS
cDNA microarray technology was adopted in the analysis of gene expression of cultured human umbilical vein endothelial cells (HUVECs) that had been incubated by 17 beta-estradiol for 48 h and then exposed to low fluid shear stress (4.20 dyne/cm2) for 2 h, Normal static cultured HUVECs were selected as control. Total RNA from normal static cultured HUVECs was labeled by Cy3-dCTP, and the total RNA of HUVECs incubated by 17 beta-estradiol (10(-7) mol/L) and then exposed to low shear stress was labeled by Cy5-dCTP. The expression ratios reported are the average from the two separate experiments.


RESULTS
A total of 384 genes (approximately 0.094%) exhibited differential expression. 226 genes were up-regulated and 158 genes were down-regulated. The transcription protein of up-regulated genes related to synthesis of PGI2, macrophage phagocytosis, and so on. The transcription protein of down-regulated genes related to degradation of fibrinogen, and to production of protooncogene.


CONCLUSIONS
Estrin at the physiological concentration could protect impaired endothelial cells. Its protective effect may be related with some genes.
Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu 610041, China.
Wen-sheng
WS
Zhang
Min
M
Cheng
Huai-qing
HQ
Chen
Wen-chao
WC
Wu
Hua
H
Huang
D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D011464:Epoprostenol / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005786:Gene Expression Regulation; D006801:Humans; D008264:Macrophages / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis; D010587:Phagocytosis; D013314:Stress, Mechanical; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism
D014157:Transcription Factors; D004958:Estradiol; D011464:Epoprostenol