[Polymorphisms of five short tandem repeat systems in Chinese Zang population in Kangba area].
OBJECTIVE
To get preliminary genotype and allele frequency distributions of D1S549, D3S1754, D12S391, D12S375 and D18S865 loci in Chinese Zang (Tibetan) population in Kangba area and to validate more short tandam repeat (STR) systems for forensic application.


METHODS
Fresh samples of venous blood from 100 unrelated individuals of Chinese Zang population in Kangba area were dropped on the filter paper. After they dried up, DNA were extracted by chelex-100 method and amplified by the polymerase chain reaction (PCR). The PCR products were analyzed by PAG vertical electrophoresis and silver staining.


RESULTS
Seven alleles were found at D1S549 locus, six alleles at D3S1754 locus, ten alleles at D12S391 locus, six alleles at D12S375 and six alleles at D18S865 locus. No deviations from Hardy-Weinberg equilibrium were observed. The heterozygosities observed were 0.86, 0.77, 0.81, 0.69, 0.80 for D1S549, D3S1754, D12S391, D12S375 and D18S865 respectively. The calculated values of the Power of Exclusion were 0.715, 0.545, 0.618, 0.413 and 0.599, and the values of the Power of Discrimination were 0.929, 0.838, 0.934, 0.886 and 0.890, respectively.


CONCLUSIONS
All of the five loci in this study were useful for individual identification and paternity test, as well as for research purposes in genetics.
Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Yu
Y
Hu
Miao
M
Liao
Bin
B
Zhou
Yi
Y
Jia
Lin
L
Zhang
Guo-di
GD
Chen
D000483:Alleles; D002681:China / Q000208:ethnology; D004591:Electrophoresis, Polyacrylamide Gel; D005787:Gene Frequency; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences / Q000235:genetics*
D005819:Genetic Markers