HPLC-PAD-APCI/MS assay of phenylpropanoids in cereals.
A new, rapid HPLC-PAD-APCI/MS assay has been developed in order to measure accurately the amount of p-coumaric, E- and Z-ferulic acid and the dehydrodimers of ferulic acid in cereal grain. In the positive ionisation mode, MS patterns gave additional information for the identification of the dimers. The time required and the quantities of solvents employed in the developed analytical method are much lower than those involved in previously available assays of these compounds, thus making the method suitable for the screening of cereal genotypes. Application of the method to accessions of maize, wheat and sorghum showed that E-ferulic was the most abundant phenylpropanoid, whilst the major dimer was 8-O-4' dehydrodimer of ferulic acid followed by the 5-5' and then the 8-5' forms. Maize grains, especially of the Mexican landraces, contained the highest levels of these dimers.
Bily
Antoine C
AC
Ottawa-Carleton Institute of Biology, University of Ottawa, 30 Marie Curie, PO Box 450, Stn A, Ottawa, Ontario, Canada K1N 6N5.
Burt
Andrew J
AJ
Ramputh
Al-Idrissi
AI
Livesey
John
J
Regnault-Roger
Catherine
C
Philogène
Bernard R
BR
Arnason
John T
JT
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000032:analysis*; D002523:Edible Grain / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D012275:Oryza / Q000737:chemistry; D011422:Propionates; D045868:Sorghum / Q000737:chemistry; D014908:Triticum / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry
D003373:Coumaric Acids; D011422:Propionates; C004999:ferulic acid; C495469:p-coumaric acid