Two-photon imaging of brain pericytes in vivo using dextran-conjugated dyes.
Pericytes in the central nervous system (CNS) are hypothesized to be involved in important circulatory functions, including local blood flow regulation, angiogenesis, immune reaction, and regulation of blood-brain barrier. Despite these putative functions, functional correlates of pericytes in vivo are scarce. We have labeled CNS pericytes using the dextran-conjugated fluorescent calcium indicator Calcium Green I and imaged them in somatosensory cortex of the mouse in vivo. Intracellular calcium concentration in pericytes showed spontaneous surges lasting for several seconds. Furthermore, population bursts of neuronal activity were associated with increased Ca(2+) signal in a portion of the pericytes. Selective in vivo labeling of pericytes with functional markers may help reveal their physiological function in neuronal activity-associated regulation of local cerebral blood flow.
Hirase
Hajime
H
Center for Molecular and Behavioral Neuroscience, Rutgers, State University of New Jersey, Newark, New Jersey 07102, USA.
Creso
Judith
J
Singleton
Malaika
M
Barthó
Peter
P
Buzsáki
György
G
D000818:Animals; D001923:Brain Chemistry / Q000502:physiology; D003911:Dextrans / Q000032:analysis*; D005260:Female; D005456:Fluorescent Dyes / Q000032:analysis*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal / Q000379:methods; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods; D009930:Organic Chemicals; D020286:Pericytes / Q000737:chemistry* / Q000166:cytology / Q000502:physiology
D003911:Dextrans; D005456:Fluorescent Dyes; D009930:Organic Chemicals; C071336:calcium green