CD43 has a functional NLS, interacts with beta-catenin, and affects gene expression.
CD43 is a transmembrane molecule with a highly O-glycosylated extracellular domain of mucin type. It is a normal constituent of leukocytes and found in colon adenoma, but not in normal colon epithelia. Here it is shown that the cytoplasmic tail of CD43 contains a functional bipartite nuclear localization signal directing it to the nucleus. The intracellular domain of CD43 interacts with beta-catenin and causes an upregulation of the beta-catenin target genes c-MYC and CyclinD1. The present results suggest that CD43 can be involved in nuclear signaling and via beta-catenin interaction be involved in cell proliferation.
Department of Medical Biochemistry, Göteborg University, Medicinaregatan 9A, 413 90 Gothenburg, Sweden.
Christian X
CX
Andersson
Julia
J
Fernandez-Rodriguez
Sirle
S
Laos
Rein
R
Sikut
Anu
A
Sikut
Dan
D
Baeckström
Gunnar C
GC
Hansson
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D051917:Leukosialin; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals; D012795:Sialoglycoproteins / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*; D015534:Trans-Activators / Q000378:metabolism*; D015533:Transcriptional Activation; D051176:beta Catenin
D015703:Antigens, CD; D003598:Cytoskeletal Proteins; D051917:Leukosialin; D019913:Nuclear Localization Signals; D012795:Sialoglycoproteins; D015534:Trans-Activators; D051176:beta Catenin