An allele-specific genotyping method for rat lyst (lysosomal trafficking regulator) gene.
Two spontaneous mutant beige rats, with phenotypes resembling human Chediak- Higashi syndrome (CHS), were found independently in two inbred strains. Both beige mutations were identified to be recessive alleles in the Lyst locus on rat chromosome 17 and the alleles were denoted Lyst(bg) and Lyst(bg-Kyo). As it is almost impossible to discriminate these mutations phenotypically, we developed an allele-specific genotyping method for the Lyst gene. The nested PCR amplification was followed by restriction fragment length polymorphism (RFLP) analysis. By this method, we could discriminate the mutant Lyst(bg), Lyst(bg-Kyo) alleles, and the normal Lyst allele, easily and accurately.
Japan SLC, Inc., Aoihigashi, Hamamatsu, Shizuoka, Japan.
Norio
N
Masui
Masayuki
M
Mori
Tetsu
T
Nishikawa
Yumie
Y
Takagi
Hidekazu
H
Asai
Makoto
M
Yanabe
Ken-Ichi
K
Yamasaki
Tadao
T
Serikawa
Katsunori
K
Sato
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004586:Electrophoresis; D005808:Genes, Recessive / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D051381:Rats
D017931:DNA Primers