[Analysis of the influence of Ca(OH)2 and HA on cell cycle of pulp cells by flow cytometry].
OBJECTIVE
The influence of Ca(OH)(2) (calcium hydroxide) and HA(hydroxyapatite) as pulp capping agents on cell cycle of pulp cells was analyzed by flow cytometry.


METHODS
Permanent teeth pulps of pigs were capped with Ca(OH)(2), and HA following mechanical pulp exposure. The teeth were extracted after 2, 4, 8 and 12 weeks, respectively. The pulps were taken and digested by collagenase. The percentages of pulp cells in G0/G1, G2, S and M periods were determined by flow cytometry and computer analysis.


RESULTS
The percentage of cells in G0/G1, period was decreased, whereas the percentage of cells in S period was increased after pulp capping with Ca(OH)(2) and HA.


CONCLUSIONS
This study indicated that Ca(OH)(2) and HA could increase DNA synthesis of pulp cells.
Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China.
J N
JN
Zhang
X Y
XY
Wang