Comparison of preparative HPLC/MS and preparative SFC techniques for the high-throughput purification of compound libraries.
A diverse set of 16 high-throughput organic synthesis libraries, consisting of 48 samples per library, has been purified by both preparative supercritical fluid chromatography (SFC) and preparative high-performance liquid chromatography (HPLC). This paper details the relative effectiveness of these two purification techniques in terms of success, yield, and purity of final product.
Medicinal Chemistry Technologies, Global Pharmaceutical Research & Development, Abbott Laboratories, Department R4CP, Building AP10, 100 Abbott Park Road, Abbott Park, IL 60064-6101, USA. philip.searle@abbott.com
Philip A
PA
Searle
Keithney A
KA
Glass
Jill E
JE
Hochlowski