Cryoelectron microscopy analysis of the structural changes associated with human rhinovirus type 14 uncoating.
Release of the human rhinovirus (HRV) genome into the cytoplasm of the cell involves a concerted structural modification of the viral capsid. The intracellular adhesion molecule 1 (ICAM-1) cellular receptor of the major-group HRVs and the low-density lipoprotein (LDL) receptor of the minor-group HRVs have different nonoverlapping binding sites. While ICAM-1 binding catalyzes uncoating, LDL receptor binding does not. Uncoating of minor-group HRVs is initiated by the low pH of late endosomes. We have studied the conformational changes concomitant with uncoating in the major-group HRV14 and compared them with previous results for the minor-group HRV2. The structure of empty HRV14 was determined by cryoelectron microscopy, and the atomic structure of native HRV14 was used to examine the conformational changes of the capsid and its constituent viral proteins. For both HRV2 and HRV14, the transformation from full to empty capsid involves an overall 4% expansion and an iris type of movement of viral protein VP1 to open up a 10-A-diameter channel on the fivefold axis to allow exit of the RNA genome. The beta-cylinders formed by the N termini of the VP3 molecules inside the capsid on the fivefold axis all open up in HRV2, but we propose that only one opens up in HRV14. The release of VP4 is less efficient in HRV14 than in HRV2, and the N termini of VP1 may exit at different points. The N-terminal loop of VP2 is modified in both viruses, probably to detach the RNA, but it bends only inwards in HRV2.
Hewat
Elizabeth A
EA
Institut de Biologie Structurale Jean-Pierre Ebel, 38027 Grenoble, France. elizabeth.hewat@ibs.fr
Blaas
Dieter
D
D002213:Capsid / Q000648:ultrastructure*; D036022:Capsid Proteins / Q000648:ultrastructure; D020285:Cryoelectron Microscopy / Q000295:instrumentation / Q000379:methods*; D018360:Crystallography, X-Ray; D006367:HeLa Cells; D006801:Humans; D021621:Imaging, Three-Dimensional; D008958:Models, Molecular; D011487:Protein Conformation; D012229:Rhinovirus / Q000378:metabolism / Q000648:ultrastructure*
D036022:Capsid Proteins