Detection of rinderpest virus using N-protein monoclonal antibodies.
A panel of monoclonal antibodies (mAbs) was generated against the RBOK strain of rinderpest virus (RPV). All of them bound to the N protein of RPV. The antigen capture ELISA using the mAbs could detect the virus in crude viral preparations. The mAb 12BF8.1.1 showed higher reactivity with cell-associated (CA) virus, whereas the mAbs 12AD10.1.1, 12BD7.1.1 and 12DG7.1.1 showed higher reactivity with extracellular virus (hereafter referred to as cell-free (CF) virus). The mAbs 12BF8.1.1 and 12AD10.1.1 could detect the virus in infected Vero cell culture supernatants (CCS) as early as 24 h post-cytopathic effect (CPE) initiation. Detergent treatment (Triton X-100) of RPV preparations enhanced the binding of the mAbs to the virus. All the seven mAbs showed specific fluorescence in virus-infected cell cultures. The immunofluorescence (IFA) using mAbs was found to be more sensitive and reliable than the immunoperoxidase test (IPT) for detection of rinderpest.
Shah
R A
RA
National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar 243 122, UP, India.
Joseph
M C
MC
Butchaiah
G
G
Malik
M
M
Singh
R K
RK
Bakshi
C S
CS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western / Q000662:veterinary; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D007124:Immunoenzyme Techniques / Q000662:veterinary; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests / Q000662:veterinary; D019590:Nucleocapsid Proteins / Q000276:immunology*; D012301:Rinderpest / Q000175:diagnosis* / Q000821:virology; D012302:Rinderpest virus / Q000276:immunology* / Q000302:isolation & purification; D014709:Vero Cells
D000911:Antibodies, Monoclonal; D019590:Nucleocapsid Proteins