Gingipain adhesin domains mediate Porphyromonas gingivalis adherence to epithelial cells.
Porphyromonas gingivalis, a Gram-negative oral anaerobe, interacts with epithelium lining the gingival sulcus. Continuing our studies on the role of gingipain cysteine proteinases in P. gingivalis adherence to epithelial cells, we showed that antibody raised to the recombinant adhesin domain of arg-gingipain A blocked bacterial attachment, providing new additional evidence that P. gingivalis adherence to epithelial cells is mediated by gingipain adhesin peptides.
Chen
Tsute
T
Department of Molecular Genetics, The Forsyth Institute, 140 Fenway, Boston, MA 02115, USA.
Duncan
Margaret J
MJ
D018829:Adhesins, Bacterial / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D000907:Antibodies, Bacterial / Q000276:immunology; D001422:Bacterial Adhesion / Q000502:physiology*; D002460:Cell Line; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D004847:Epithelial Cells / Q000382:microbiology*; D005798:Genes, Bacterial; D000080867:Gingipain Cysteine Endopeptidases; D006388:Hemagglutinins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D016966:Porphyromonas gingivalis / Q000201:enzymology* / Q000378:metabolism / Q000472:pathogenicity*; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000080867:Gingipain Cysteine Endopeptidases; D006388:Hemagglutinins; D011994:Recombinant Proteins; D003546:Cysteine Endopeptidases