A parvovirus isolated from royal python (Python regius) is a member of the genus Dependovirus.
Parvoviruses were isolated from Python regius and Boa constrictor snakes and propagated in viper heart (VH-2) and iguana heart (IgH-2) cells. The full-length genome of a snake parvovirus was cloned and both strands were sequenced. The organization of the 4432-nt-long genome was found to be typical of parvoviruses. This genome was flanked by inverted terminal repeats (ITRs) of 154 nt, containing 122 nt terminal hairpins and contained two large open reading frames, encoding the non-structural and structural proteins. Genes of this new parvovirus were most similar to those from waterfowl parvoviruses and from adeno-associated viruses (AAVs), albeit to a relatively low degree and with some organizational differences. The structure of its ITRs also closely resembled those of AAVs. Based on these data, we propose to classify this virus, the first serpentine parvovirus to be identified, as serpentine adeno-associated virus (SAAV) in the genus Dependovirus.
Farkas
Szilvia L
SL
Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, H-1581 Budapest, Hungary.
Zádori
Zoltán
Z
INRS-Institut Armand-Frappier, Université du Québec, 531 boul. des Praires, Laval, Quebec, Canada H7V 1B7.
Benkő
Mária
M
Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, H-1581 Budapest, Hungary.
Essbauer
Sandra
S
Institute for Zoology, Fish Biology, Fish Diseases, University of Munich, Germany.
Harrach
Balázs
B
Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, H-1581 Budapest, Hungary.
Tijssen
Peter
P
INRS-Institut Armand-Frappier, Université du Québec, 531 boul. des Praires, Laval, Quebec, Canada H7V 1B7.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017821:Boidae / Q000145:classification / Q000821:virology*; D016384:Consensus Sequence; D017931:DNA Primers; D000229:Dependovirus / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D005814:Genes, Viral; D006321:Heart / Q000821:virology; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D015183:Restriction Mapping; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D017931:DNA Primers; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins