[Studies on DNA damage in workers with long-term exposure to lower concentration of carbon disulfide].
OBJECTIVE
DNA damage in human buccal cells of workers occupationally long-term exposed to carbon disulfide (CS(2)) was monitored with comet assay.


METHODS
Ninety workers exposed to CS(2) were randomly selected as exposure group from a large-scale chemical fiber manufacturer in Hubei and 81 workers not exposed to CS(2) as control group. DNA damage in their buccal cells was detected with comet assay.


RESULTS
Rate of DNA tail was 0.51% in exposure group, significantly higher than that in control group (0.23%), with very statistical significance. Rate of DNA tail was 0.50% in male exposure group and 0.56% in exposure group with short length of employment, significantly higher than that in control group (0.08% and 0.25%, respectively). Multivariate unconditional logistic regression analysis showed that possibility of DNA damage was significantly higher in exposure group than that in control group (P < 0.05).


CONCLUSIONS
Long-term exposure to lower concentration of carbon disulfide could cause certain damage to human buccal cell DNA.
Chen
Xiao-qing
XQ
Department of Hygiene, School of Public Health, Wuhan University, Hubei 430071, China.
Tan
Xiao-dong
XD
D000328:Adult; D002246:Carbon Disulfide / Q000506:poisoning*; D016022:Case-Control Studies; D020552:Comet Assay; D004247:DNA / Q000187:drug effects / Q000235:genetics; D004249:DNA Damage; D005260:Female; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D016273:Occupational Exposure / Q000009:adverse effects*; D012307:Risk Factors; D013997:Time Factors
D004247:DNA; D002246:Carbon Disulfide