Fluorometric measurements of conformational changes in glutamate transporters.
Glutamate transporters remove glutamate from the synaptic cleft to maintain efficient synaptic communication between neurons and to prevent extracellular glutamate concentrations from reaching neurotoxic levels (1). It is thought that glutamate transporters mediate glutamate transport through a reaction cycle with conformational changes between the two major access states that alternatively expose glutamate-binding sites to the extracellular or to the intracellular solution. However, there is no direct real-time evidence for the conformational changes predicted to occur during the transport cycle. In the present study, we used voltage-clamp fluorometry to measure conformational changes in the neuronal excitatory amino acid transporter (EAAT) 3 glutamate transporter covalently labeled with a fluorescent reporter group. Alterations in glutamate and cotransported ion concentrations or in the membrane voltage induced changes in the fluorescence that allowed detection of conformational rearrangements occurring during forward and reverse transport. In addition to the transition between the two major access states, our results show that there are significant Na(+)-dependent conformational changes preceding glutamate binding. We furthermore show that Na(+) and H(+) are cotransported with glutamate in the forward part of the transport cycle. The data further suggest that an increase in proton concentrations slows the reverse transport of glutamate, which may play a neuro-protective role during ischemia.
Larsson
H Peter
HP
Neurological Sciences Institute, Oregon Health & Science University, 505 Northwest 185th Avenue, Beaverton, OR 97006, USA. larssonp@ohsu.edu
Tzingounis
Anastassios V
AV
Koch
Hans P
HP
Kavanaugh
Michael P
MP
D027322:Amino Acid Transport System X-AG / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D002623:Chemistry Techniques, Analytical; D005470:Fluorometry; D050491:Glutamate Plasma Membrane Transport Proteins; D008094:Lithium / Q000378:metabolism; D008564:Membrane Potentials; D009865:Oocytes; D018408:Patch-Clamp Techniques; D011487:Protein Conformation; D011522:Protons; D012964:Sodium / Q000378:metabolism; D027981:Symporters / Q000737:chemistry* / Q000378:metabolism; D014981:Xenopus
D027322:Amino Acid Transport System X-AG; D050491:Glutamate Plasma Membrane Transport Proteins; D011522:Protons; D027981:Symporters; D008094:Lithium; D012964:Sodium