Quantitative estimate of the effect of cellulase components during degradation of cotton fibers.
A comprehensive mechanistic kinetic model for enzymatic degradation of cotton fibers has been established based on a complete factorial experiment in combination with multivariate stepwise regression analysis. The analysis of the statistical parameter value in the model suggests that the enzymatic degradation of cotton fiber is a progressive and heterogeneous process that includes, at least, two courses that occur sequentially and then progress in parallel. Cellulose fibers were first depolymerized or solubilized by the synergism between cellobiohydrolase I (CBHI) and endoglucanase I (EGI), and then the oligomers obtained were randomly hydrolyzed into glucose by EGI and beta-glucosidase. The proposed model can be applied to the quantitative estimation of the effects of three cellulase components, CBHI, EGI, and beta-glucosidase separately, or in combination during the entire process of cellulose degradation. The validity of the proposed model has been verified by a filter paper activity assay. Its other applicability was also discussed.
Wang
Lu-Shan
LS
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
Zhang
Yu-Zhong
YZ
Yang
Hong
H
Gao
Pei-Ji
PJ
D002480:Cellulase / Q000737:chemistry* / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000378:metabolism; D032561:Cotton Fiber; D044644:Endo-1,3(4)-beta-Glucanase / Q000378:metabolism; D005374:Filtration; D005947:Glucose / Q000378:metabolism; D003368:Gossypium / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D015999:Multivariate Analysis; D012995:Solubility; D014242:Trichoderma / Q000201:enzymology; D001617:beta-Glucosidase / Q000378:metabolism
D002482:Cellulose; D001617:beta-Glucosidase; D002480:Cellulase; D044644:Endo-1,3(4)-beta-Glucanase; D043366:Cellulose 1,4-beta-Cellobiosidase; D005947:Glucose