Characterization of six type A strains of Clostridium botulinum that contain type B toxin gene sequences.
Six Clostridium botulinum isolates exhibiting type A toxicity as measured by the mouse bioassay were found to contain both type A and type B neurotoxin DNA sequences. The six strains were divided into three groups based on the DNA sequence of the type B neurotoxin gene. Members of each group exhibited 100% sequence identity over the 3876 bp type B toxin open reading frame. The type B toxin sequence of all groups differed at more than 60 positions when compared to the BGB control strain.
Kirma
Nameer
N
Department of Biology, P.O. Box 4010, Georgia State University, Atlanta, GA 30302-4010, USA. nkirma@emory.edu
Ferreira
Joseph L
JL
Baumstark
Barbara R
BR
D000818:Animals; D001483:Base Sequence; D001681:Biological Assay; D001905:Botulinum Toxins / Q000235:genetics*; D019274:Botulinum Toxins, Type A / Q000235:genetics; D003014:Clostridium botulinum / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D014774:Virulence / Q000235:genetics
D012333:RNA, Messenger; C096323:rimabotulinumtoxinB; D001905:Botulinum Toxins; D019274:Botulinum Toxins, Type A