Capillary electrochromatographic analysis of barbiturates in serum.
A capillary electrochromatographic method was developed for the separation of barbiturates. The separation was optimized in a 75 microm ID capillary, packed with 3-(1,8-naphthalimido)propyl-modified silyl silica gel (NAIP), studying the effect of buffer pH, buffer concentration, and mobile phase composition. Using an applied voltage of 20 kV and the short-end injection method (9 cm capillary effective length), the mobile phase of 1.0 mM citrate buffer (pH 5.0) containing 40% methanol provided the baseline separation of barbital, phenobarbital, secobarbital, and thiopental (internal standard) in less than 4.5 min. The method was successfully applied to the analysis of barbiturates in human serum. Under the optimal conditions, good repeatability and linearity were obtained in the range of 2.90-43.29 microg/mL for barbital, phenobarbital, and secobarbital.
Ohyama
Kaname
K
Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, Nagasaki, Japan.
Wada
Mitsuhiro
M
Lord
Gwyn A
GA
Ohba
Yoshihito
Y
Fujishita
Osamu
O
Nakashima
Kenichiro
K
Lim
Chang Kee
CK
Kuroda
Naotaka
N
D001463:Barbiturates / Q000097:blood* / Q000302:isolation & purification; D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D000432:Methanol / Q000737:chemistry; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry; D013995:Time
D001463:Barbiturates; D002021:Buffers; D012822:Silicon Dioxide; D000432:Methanol