Molecular cloning and expression of a mammalian homologue of a translationally controlled tumor protein (TCTP) gene from Penaeus monodon shrimp.
White spot syndrome, caused by white spot syndrome virus (WSSV), is a deadly disease of shrimps, causing a catastrophic loss in shrimp industries worldwide. In order to investigate molecular response of shrimp haemocyte to WSSV infection, we performed subtraction hybridization of mRNAs from healthy and WSSV-infected haemocyte. One of the genes that were severely down-regulated in moribund WSSV-infected-haemocyte was translationally controlled tumor protein (TCTP) (or fortilin). Strikingly, while there was a slight difference in the amount of TCTP message between normal and early WSSV-infected shrimps, shrimps that exhibited severe symptoms uniformly had very little TCTP in their haemocyte. Taken together with the fact that TCTP functions as an anti-apoptotic protein in mammals, our data suggest that TCTP in shrimp protects WSSV-infected shrimps from death.
Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90110, Thailand.
Phuwadol
P
Bangrak
Potchanapond
P
Graidist
Wilaiwan
W
Chotigeat
Amornrat
A
Phongdara
D000595:Amino Acid Sequence; D000818:Animals; D014408:Biomarkers, Tumor / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D002118:Calcium / Q000737:chemistry; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D008322:Mammals; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D011485:Protein Binding; D000090324:Tumor Protein, Translationally-Controlled 1
D014408:Biomarkers, Tumor; D000090324:Tumor Protein, Translationally-Controlled 1; D002118:Calcium