A LC/MS method for the determination of cyanobacteria toxins in water.
The cyanobacteria toxins anatoxin-a, microcystin-LR, microcystin-RR, microcystin-YR, and nodularin were separated in less than 30 min on several 1 mm x 15 cm reversed-phase liquid chromatography (LC) columns, and their electrospray mass spectra were measured using injections of 50 ng or less with a benchtop time-of-flight (TOF) mass spectrometer. New data from this work include the following: (a) the impact of acetic acid concentrations in the methanol-water mobile phase on measured ion abundances; (b) the performance of the electrospray-TOF mass spectrometer as an LC detector; (c) the accuracy and precision of exact m/z measurements after LC separation with a routinely used mass spectrometer resolving power of 5000; and (d) recoveries of the five toxins from reagent water, river waters, and sewage treatment plant effluent samples extracted with C-18 silica particles enmeshed in thin Teflon membrane filter disks. This technique has the potential of providing a relatively simple and reasonable-cost sample preparation and LC/MS method that provides the sensitivity, selectivity, reliability, and information content needed for source and drinking water occurrence and human exposure studies.
Oak Ridge Institute for Science and Education, 26 West Martin L. King Jr. Drive, Cincinnati, Ohio 45268, USA.
Mila
M
Maizels
William L
WL
Budde
D019342:Acetic Acid; D001427:Bacterial Toxins / Q000032:analysis* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D000087522:Cyanobacteria Toxins; D003500:Cyclization; D008387:Marine Toxins / Q000032:analysis* / Q000737:chemistry; D000432:Methanol; D052998:Microcystins; D015394:Molecular Structure; D012107:Research Design; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D014326:Tropanes; D014867:Water / Q000737:chemistry*
D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D008387:Marine Toxins; D052998:Microcystins; D014326:Tropanes; D014867:Water; C509783:anatoxin a; D019342:Acetic Acid; D000432:Methanol