Evaluation of groEL gene analysis for identification of Borrelia burgdorferi sensu lato.
The nucleotide sequences of the groEL genes, the flagellin genes, and the 16S rRNA genes from 22 reference strains of Borrelia were compared. groEL sequence analysis is useful not only in interspecies differentiation but also in intraspecies differentiation of Borrelia afzelii and Borrelia garinii isolates.
Park
Hyo-Soon
HS
Department of Microbiology, College of Medicine, Konkuk University, Chungju, Chungchungbuk-Do 380-701, Korea.
Lee
Jung-Hee
JH
Jeong
Eun-Ju
EJ
Koh
Seong-Eun
SE
Park
Tae-Kyu
TK
Jang
Won-Jong
WJ
Park
Kyung-Hee
KH
Kim
Bum-Joon
BJ
Kook
Yoon-Hoh
YH
Lee
Seung-Hyun
SH
D000818:Animals; D001483:Base Sequence; D001898:Borrelia / Q000145:classification / Q000235:genetics; D025065:Borrelia burgdorferi / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D018834:Chaperonin 60 / Q000235:genetics*; D017931:DNA Primers; D005408:Flagellin / Q000235:genetics; D006801:Humans; D018884:Ixodes / Q000382:microbiology; D008193:Lyme Disease / Q000175:diagnosis; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012867:Skin / Q000382:microbiology; D013045:Species Specificity
D018834:Chaperonin 60; D017931:DNA Primers; D005408:Flagellin