[Effects of millimeter wave on gap junctional intercellular communication in human keratinocytes].
OBJECTIVE
To explore the effect of millimeter wave (MW) at low power density on gap junctional intercellular communication (GJIC) in human keratinocytes (HaCaTs).


METHODS
Fluorescence recovery after photobleaching (FRAP) technique was employed to determine effect of 30.16 GHz MW exposure at 1.0 and 3.5 mW/cm(2) on GJIC with laser confocal scanning microscope.


RESULTS
FRAP analysis revealed that 12-O-tetradecanoylphorbol-13-acetate (TPA) at a dose of 5 microg/L could inhibit GJIC in HaCaTs. Fluorescence recovery rate fell from (55 +/- 17)% in the controls to (34 +/- 13)% after photobleaching, with a very significant difference (P < 0.001). Exposure to MW alone for one hour at either 1.0 mW/cm(2) or 3.5 mW/cm(2) did not affect GJIC, with fluorescence recovery rates of (52 +/- 16)% and (50 +/- 17)%, respectively. GJIC suppression induced by TPA was weakened by MW combined with 5 microg/L TPA treatment for one hour, which could be partially recovered by exposure to 1.0 mW/cm(2) MW with fluorescence recovery rate of (47 +/- 16)%, P < 0.01, and fully recovered by exposure to 3.5 mW/cm(2) MW with fluorescence recovery rate of (50 +/- 16)%, P < 0.001, with a very significant difference.


CONCLUSIONS
GJIC suppression induced by TPA could be eliminated or diminished by exposure to millimeter wave in HaCaTs.
Zhejiang Provincial Key Laboratory of Bioelectromagnetics, Zhejiang University School of Medicine, Hangzhou 310031, China.
Qing
Q
Chen
Qun-li
QL
Zeng
De-qiang
DQ
Lu
Huai
H
Jiang
D002450:Cell Communication / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods; D017629:Gap Junctions / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000502:physiology*; D008872:Microwaves / Q000009:adverse effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D013755:Tetradecanoylphorbol Acetate