[Noise magnetic fields block co-suppression effect induced by power frequency magnetic field and phorbol ester].
OBJECTIVE
To explore intervention with electromagnetic noise for co-suppression effect on gap-junctional intercellular communication (GJIC) induced or strengthened by low intensity magnetic field with carcinogen 12-O-tetradecanoylphorbol-13-acetate (TPA).


METHODS
Fibroblast cells from NIH 3T3 mice were exposed to extremely low intensity magnetic field (MF) 0.2 mT, 0.2 mT + TPA or/and electromagnetic noise with the same intensity of MF for 24 h, and GJIC was determined using fluorescence recovery analysis after photobleaching (FRAP) with a laser-scanning confocal microscope (Leica, Germany).


RESULTS
GJIC function could be co-suppressed by MF of 0.2 mT with TPA, with fluorescence recovery of (23 +/- 11)%, lower than that in the control group [(46 +/- 19)%] and in the group with TPA only [(34 +/- 17) %] (P < 0.01), indicating 0.2 mT MF plus TPA could co-inhibit GJIC (P < 0.01). Superposition of 0.2 mT noise MF could get a fluorescence recovery of (35 +/- 19)% and significantly antagonize its co-suppression by TPA.


CONCLUSIONS
Electromagnetic noise of 0.2 mT could block the intensifying effect of power frequency magnetic field on TPA-induced GJIC inhibition.
Zhejiang Provincial Key Laboratory of Bioelectromagnetics, Zhejiang University School of Medicine, Hangzhou 310031, China.
Xiang-wei
XW
Gao
Zheng-ping
ZP
Xu
Ya-nan
YN
Huo
Huai
H
Jiang
Yi-ti
YT
Fu
De-qiang
DQ
Lu
Qun-li
QL
Zeng
D000818:Animals; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D002460:Cell Line; D004574:Electromagnetic Fields / Q000009:adverse effects*; D036681:Fluorescence Recovery After Photobleaching; D017629:Gap Junctions / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D051379:Mice; D041681:NIH 3T3 Cells; D009622:Noise / Q000009:adverse effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D013755:Tetradecanoylphorbol Acetate