Expression of cone-photoreceptor-specific antigens in a cell line derived from retinal tumors in transgenic mice.
OBJECTIVE
To examine an immortalized mouse retinal cell line (661W) for markers characteristic of photoreceptor cells and validate its photoreceptor origin.


METHODS
The 661W cells were cloned from retinal tumors of a transgenic mouse line that expresses the simian virus (SV) 40 T antigen under control of the human interphotoreceptor retinol-binding protein (IRBP) promoter. Morphologic, immunocytochemical, and immunoblot analyses were performed to characterize these cells. Total cellular protein was used for immunoblot analysis of various photoreceptor-specific proteins.


RESULTS
661W cells grew as a monolayer and exhibited processes characteristic of neuronal cells. Immunoblot analysis showed that 661W cells expressed SV40 T antigen, blue and green cone pigments, transducin, and cone arrestin. Immunocytochemical detection of blue and green opsins showed distribution throughout the cell, the nucleus included. However, these cells did not express rod-specific antigens, such as opsin and arrestin or rod- and cone-specific proteins such as phosducin, peripherin/rds, and ROM1. Furthermore, the cells did not express RPE65, a cone- and RPE-cell-specific protein.


CONCLUSIONS
661W cells demonstrate cellular and biochemical characteristics exhibited by cone photoreceptor cells. These cells also resemble neuronal cells with their spindlelike processes and should serve as a useful alternative in vitro model for the study of cone photoreceptor cell biology and associated diseases.
Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
Elaine
E
Tan
Xi-Qin
XQ
Ding
Anisse
A
Saadi
Neeraj
N
Agarwal
Muna I
MI
Naash
Muayyad R
MR
Al-Ubaidi
D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism*; D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism; D019393:Arrestin / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D002352:Carrier Proteins; D005136:Eye Proteins / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D020555:GTP-Binding Protein Regulators; D051379:Mice; D008822:Mice, Transgenic; D010750:Phosphoproteins / Q000378:metabolism; D011506:Proteins; D017949:Retinal Cone Photoreceptor Cells / Q000378:metabolism*; D019572:Retinal Neoplasms / Q000235:genetics / Q000378:metabolism*; D017299:Rod Opsins / Q000378:metabolism; D015293:Transducin / Q000378:metabolism; D014407:Tumor Cells, Cultured; D019745:cis-trans-Isomerases; D000099178:Retinoid Isomerohydrolase
D000943:Antigens, Differentiation; D000952:Antigens, Polyomavirus Transforming; D019393:Arrestin; D015415:Biomarkers; D002352:Carrier Proteins; D005136:Eye Proteins; D020555:GTP-Binding Protein Regulators; D010750:Phosphoproteins; D011506:Proteins; D017299:Rod Opsins; D015293:Transducin; D019745:cis-trans-Isomerases; D000099178:Retinoid Isomerohydrolase; C065631:phosducin