Paeonol inhibits anaphylactic reaction by regulating histamine and TNF-alpha.
Paeonol, a major phenolic component of Moutan Cortex, was known to have antiaggregatory, antioxidant and antiinflammatory activities. In the present study, we tried to elucidate the effects of paeonol on anaphylactic reaction and its mode of action. Paeonol significantly inhibited histamine release from the rat peritoneal mast cells (RPMCs) treated with compound 48/80, a mast cell degranulator. The release of tumor necrosis factor (TNF)-alpha mast cell activating cytokine was significantly suppressed in RBL-2H3 mast cells pretreated with anti-dinitrophenyl (DNP) immunoglobulin E (IgE) in a dose-dependent manner. Paeonol significantly inhibited IgE production in B cells activated by anti-CD40 mAb, recombinant interleukin-4 (rIL-4) and recombinant histamine releasing factor (rHRF). Paeonol effectively downregulated the expression of IL-4 in the activated B cells by reverse transcription-polymerase chain reaction (RT-PCR). We also confirmed that paeonol effectively inhibited anaphylactic shock in mice by 90% at a dose of 0.5 mg/mouse versus PBS treated control 2 h after the i.p. injection of compound 48/80. These results suggest that paeonol has antianaphylatic activity by regulating histamine and TNF-alpha.
Department of Oncology, Graduate School of East-West Medical Science, KyungHee University, 1 Seochunri Kiheungeup, Yoingin 449-701, South Korea. sungkim7@khu.ac.kr
Sung Hoon
SH
Kim
Seung-Ae
SA
Kim
Mi-Kyung
MK
Park
Seung-Hyung
SH
Kim
Young-Doo
YD
Park
Ho-Jeong
HJ
Na
Hyung-Min
HM
Kim
Min-Kyu
MK
Shin
Kyoo-Seok
KS
Ahn
D000098:Acetophenones / Q000494:pharmacology*; D000707:Anaphylaxis / Q000139:chemically induced / Q000517:prevention & control*; D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D004365:Drugs, Chinese Herbal; D006636:Histamine Release / Q000187:drug effects*; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000276:immunology; D015847:Interleukin-4 / Q000032:analysis / Q000096:biosynthesis; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D029596:Paeonia; D010529:Peritoneal Cavity / Q000166:cytology; D018517:Plant Roots; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000166:cytology; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D003189:p-Methoxy-N-methylphenethylamine
D000098:Acetophenones; D018926:Anti-Allergic Agents; D004365:Drugs, Chinese Herbal; D014409:Tumor Necrosis Factor-alpha; C038881:moutan cortex; D015847:Interleukin-4; D007073:Immunoglobulin E; C013638:paeonol; D003189:p-Methoxy-N-methylphenethylamine