Effect of dietary enrichment with n-3 polyunsaturated fatty acids (PUFA) or n-9 PUFA on arachidonate metabolism in vivo and experimentally induced inflammation in mice.
Mice were fed a diet supplemented with palm oil (control diet), n-3 polyunsaturated fatty acids (PUFA)-, or n-9 PUFA-rich oil for 3 weeks. The n-3 PUFA-rich diet suppressed the generation of both leukotrienes (LT) and prostaglandins (PG), but the n-9 PUFA-rich diet did LT but not PG generation during acute inflammation. Leukocyte accumulation during acute inflammation was not different in the n-3 or n-9 PUFA-rich diet group as compared with the control group. The n-3 PUFA-rich diet but not the n-9 PUFA-rich diet suppressed Freund's adjuvant-induced granuloma formation. The n-9 PUFA-rich diet significantly attenuated galactosamine/lipopolysaccharide-induced liver injury more effectively than the n-3 PUFA-rich diet as compared with the control diet. The present study revealed the differential modification of experimentally induced inflammation in mice by dietary n-3 PUFA and n-9 PUFA, which may be due to their different effects on 5-lipoxygenease and cyclooxygenase metabolism of arachidonic acid during inflammatory processes.
Division of Clinical Application, Department of Clinical Sciences, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Sugitani, Toyama, Japan.
Masaru
M
Doshi
Shiro
S
Watanabe
Tsuyoshi
T
Niimoto
Hiroshi
H
Kawashima
Yoshiyuki
Y
Ishikura
Yoshinobu
Y
Kiso
Tomohito
T
Hamazaki
D000208:Acute Disease; D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism; D016718:Arachidonic Acid / Q000378:metabolism*; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D004032:Diet; D015525:Fatty Acids, Omega-3; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005260:Female; D005620:Freund's Adjuvant; D005688:Galactosamine; D006099:Granuloma / Q000209:etiology / Q000517:prevention & control; D007249:Inflammation / Q000188:drug therapy* / Q000378:metabolism; D007958:Leukocyte Count; D008055:Lipids / Q000737:chemistry; D008070:Lipopolysaccharides; D016859:Lipoxygenase Inhibitors; D008107:Liver Diseases / Q000201:enzymology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010529:Peritoneal Cavity / Q000166:cytology; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D014280:Triglycerides / Q000494:pharmacology*
D016861:Cyclooxygenase Inhibitors; D015525:Fatty Acids, Omega-3; D005231:Fatty Acids, Unsaturated; D008055:Lipids; D008070:Lipopolysaccharides; D016859:Lipoxygenase Inhibitors; D014280:Triglycerides; C077446:Pikasol; D016718:Arachidonic Acid; D005688:Galactosamine; D005620:Freund's Adjuvant; D001094:Arachidonate 5-Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases