Use of PCR primers derived from a putative transcriptional regulator gene for species-specific determination of Listeria monocytogenes.
Listeria monocytogenes is an opportunistic bacterial pathogen that has accounted for an important portion of human foodborne diseases worldwide. In this study, through comparative analysis of L. innocua and L. monocytogenes genomic sequences, we selected a L. monocytogenes specific gene (lmo0733) that has the potential for specific detection of L. monocytogenes. Using PCR primers (lmo0733F and lmo0733R) derived from this gene, a specific fragment of 453 bp was amplified only from genomic DNA of L. monocytogenes strains. PCR products from other Listeria species as well as other Gram-positive and -negative species were not detectable, confirming the specificity of this assay. Thus, the PCR test employing primers lmo0733F and lmo0733R represents an additional tool in the diagnostic arsenal for rapid, sensitive and specific detection and identification of human infections due to L. monocytogenes.
Liu
Dongyou
D
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, 39762, USA.
Ainsworth
A Jerald
AJ
Austin
Frank W
FW
Lawrence
Mark L
ML
D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis*; D005784:Gene Amplification; D015964:Gene Expression Regulation, Bacterial; D008089:Listeria monocytogenes / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D014158:Transcription, Genetic / Q000235:genetics*
D017931:DNA Primers; D004269:DNA, Bacterial