Somatostatin is a specific inhibitor of SDF-1alpha-induced T cell infiltration.
The chemokine stromal cell-derived factor 1alpha (SDF-1alpha) is a potent stimulator of T cell infiltration into three-dimensional type I collagen matrices as demonstrated using T cells freshly isolated from blood and an activated T cell clone. The neuropeptide somatostatin selectively inhibits SDF-1alpha induced T cell infiltration by the same T cells including CD4 as well as CD8 positive cells, while somatostatin does not inhibit 'spontaneous' T cell infiltration. A number of other neuropeptides and opioids do not inhibit SDF-1alpha-induced T cell infiltration, indicating that the inhibitory effect is somatostatin-specific. The neuropeptide antagonist cyclosomatostatin abrogated the inhibitory effect of somatostatin on T cell infiltration, indicating that the effect of somatostatin is mediated via specific somatostatin receptors. Somatostatin does not inhibit SDF-1alpha-induced T cell attachment to the collagen substrate, which indicates that this neuropeptide specifically inhibits the process of chemokine-induced T cell penetration and migration through the collagen.
Department of Medicine, Section of Dermatology and Venereology, Karolinska Institutet at Huddinge University Hospital, Huddinge, Sweden.
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Talme
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Ivanoff
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Sundqvist
D002478:Cells, Cultured; D054377:Chemokine CXCL12; D018925:Chemokines / Q000276:immunology; D019743:Chemokines, CXC / Q000037:antagonists & inhibitors*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D009479:Neuropeptides / Q000494:pharmacology; D019707:Receptors, Chemokine / Q000378:metabolism; D013004:Somatostatin / Q000494:pharmacology*; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000276:immunology
C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D018925:Chemokines; D019743:Chemokines, CXC; D019012:Integrin beta1; D009479:Neuropeptides; D019707:Receptors, Chemokine; D013004:Somatostatin