Survival of human periodontal ligament cells in media proposed for transport of avulsed teeth.
Many solutions have been examined as possible storage media for avulsed teeth. In this report, human periodontal ligament (PDL) cells were exposed for 1 h to culture medium, milk, Hanks Balanced Salt Solution (HBSS), Soft Wear, Opti Free, and Solo Care contact lens solutions, Gatorade, and tap water, at room temperature and on ice. The number of viable cells was counted using the trypan blue exclusion technique, immediately after exposure (0 h) and at 24 and 48 h, to test the proliferative capacity of the cells after treatment. The results indicated that a significantly higher number of cells survived and proliferated when the exposures were performed at 0 degrees C. Water had a detrimental effect on the cells, whereas culture medium and HBSS preserved significantly more viable cells than the other experimental solutions. Within the parameters of this study, it appears that HBSS is the optimal storage medium for avulsed teeth. Low-fat milk could serve as an alternative if ice is available. Contact lens solutions or Gatorade on ice could serve as short-term (1 h) storage media if the other solutions are not readily available.
Department of Endodontics, Texas A&M University System Health Science Center, Dallas, TX 75246, USA.
Emmanouil
E
Sigalas
John D
JD
Regan
Phillip R
PR
Kramer
David E
DE
Witherspoon
Lynne A
LA
Opperman
D000818:Animals; D002417:Cattle; D002452:Cell Count; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D018639:Contact Lens Solutions; D003470:Culture Media; D006801:Humans; D007053:Ice; D007552:Isotonic Solutions; D008892:Milk; D019188:Organ Preservation Solutions; D010513:Periodontal Ligament / Q000166:cytology*; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods; D014084:Tooth Avulsion; D014867:Water
D018639:Contact Lens Solutions; D003470:Culture Media; C072670:Hanks Balanced Salt Solution; D007053:Ice; D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; C011259:gatorade; D014867:Water