Laboratory confirmation of generalized vaccinia following smallpox vaccination.
The reinitiation of smallpox vaccination has renewed interest in implementing modern diagnostic methods to assess orthopoxvirus infection and adverse events following vaccination. We report here the laboratory confirmation of vaccinia virus in pustular lesions of a healthy adult vaccinee by use of a two-tier algorithm incorporating TaqMan PCR and electron microscopy.
Wadsworth Center, New York State Department of Health, Albany, New York 12208, USA.
Cassandra D
CD
Kelly
Christina
C
Egan
Stephen W
SW
Davis
William A
WA
Samsonoff
Kimberlee A
KA
Musser
Peter
P
Drabkin
James R
JR
Miller
Jill
J
Taylor
Nick M
NM
Cirino
D000328:Adult; D005260:Female; D016679:Genome, Viral; D006282:Health Personnel; D014645:Herpesvirus 3, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D009519:New York City; D016133:Polymerase Chain Reaction / Q000379:methods; D012882:Skin Tests; D012900:Smallpox Vaccine / Q000009:adverse effects*; D014615:Vaccinia / Q000209:etiology / Q000473:pathology*
D012900:Smallpox Vaccine