Evaluation of a real-time PCR kit for detecting Escherichia coli O157 in bovine fecal samples.
A commercially available real-time, rapid PCR test was evaluated for its ability to detect Escherichia coli O157. Both the sensitivity and specificity of the assay were 99% for isolates in pure culture. The assay detected 1 CFU of E. coli O157:H7 g(-1) in artificially inoculated bovine feces following enrichment.
U.S. Meat Animal Research Center, Agricultural Research Service, U.S. Department of Agriculture, Clay Center, Nebraska 68933-0166, USA. bono@email.marc.usda.gov
James L
JL
Bono
James E
JE
Keen
Laura C
LC
Miller
James M
JM
Fox
Carol G
CG
Chitko-McKown
Michael P
MP
Heaton
William W
WW
Laegreid
D000818:Animals; D002417:Cattle; D019453:Escherichia coli O157 / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D005243:Feces / Q000382:microbiology*; D005516:Food Microbiology; D006801:Humans; D008460:Meat / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity