A high-resolution map of the H1 locus harbouring resistance to the potato cyst nematode Globodera rostochiensis.
The resistance gene H1 confers resistance to the potato cyst nematode Globodera rostochiensis and is located at the distal end of the long arm of chromosome V of potato. For marker enrichment of the H1 locus, a bulked segregant analysis (BSA) was carried out using 704 AFLP primer combinations. A second source of markers tightly linked to H1 is the ultra-high-density (UHD) genetic map of the potato cross SH x RH. This map has been produced with 387 AFLP primer combinations and consists of 10,365 AFLP markers in 1,118 bins (http://www.dpw.wageningen-ur.nl/uhd/). Comparing these two methods revealed that BSA resulted in one marker/cM and the UHD map in four markers/cM in the H1 interval. Subsequently, a high-resolution genetic map of the H1 locus has been developed using a segregating F(1) SH x RH population consisting of 1,209 genotypes. Two PCR-based markers were designed at either side of the H1 gene to screen the 1,209 genotypes for recombination events. In the high-resolution genetic map, two of the four co-segregating AFLP markers could be separated from the H1 gene. Marker EM1 is located at a distance of 0.2 cM, and marker EM14 is located at a distance of 0.8 cM. The other two co-segregating markers CM1 (in coupling) and EM15 (in repulsion) could not be separated from the H1 gene.
Laboratory of Nematology, The Graduate School of Experimental Plant Sciences, Wageningen University, P.O. Box 8123, 6700 ES Wageningen, The Netherlands. erin.bakker@wur.nl
Erin
E
Bakker
Ute
U
Achenbach
Jeroen
J
Bakker
Joke
J
van Vliet
Johan
J
Peleman
Bart
B
Segers
Stefan
S
van der Heijden
Piet
P
van der Linde
Robert
R
Graveland
Ronald
R
Hutten
Herman
H
van Eck
Eric
E
Coppoolse
Edwin
E
van der Vossen
Jaap
J
Bakker
Aska
A
Goverse
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D017931:DNA Primers; D007113:Immunity, Innate / Q000235:genetics*; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics / Q000469:parasitology*; D012150:Polymorphism, Restriction Fragment Length; D011198:Solanum tuberosum / Q000235:genetics*; D014431:Tylenchoidea
D017931:DNA Primers