[Measuring the shelf life of CPDA and ACD whole blood stored at above 4 degrees C in vitro].
In order to measure the shelf life of whole blood stored at above 4 degrees C and provide experimental data for blood preservation and transportation in battle fields, 200 ml whole blood was collected from each of the 10 donors and anticoagulated by CPDA or ACD, then 50 ml whole blood was separated from each one and marked as control group, the rest was marked as test group. The control group was stored at 4 degrees C and RBC ATP concentrations was measured at the end of its shelf life which signed as critical ATP. The test group was stored at above 4 degrees C condition, some items as ATP, FHb (free hemoglobin), serum K(+) and germiculture were tested daily and ensured all of them eligible. When RBC ATP decreased to the level of critical ATP, the time of preservation was considered as shelf life. The results showed that at temperatures from 10 to 33 degrees C, the shelf life of CPDA whole blood ranges from 2.5 days to 18 days, while shelf life of ACD whole blood ranges from 1 day to 13 days. It is concluded that CPDA whole blood stored at above 4 degrees C condition can be sent to the front hospital in effective shelf life so that the wounded can be cured in time.
Department of Blood Transfusion, General Hospital of PLA, Center of Clinical Transfusion of PLA, Beijing 100853, China. hanw5301@sohu.com
Wei
W
Han
Jing-Han
JH
Liu
Tie-Jun
TJ
Wang
Rui
R
Li
Xi-Lin
XL
Ouyang
Xi-Jin
XJ
Li
D000255:Adenosine Triphosphate / Q000097:blood; D001793:Blood Preservation; D004912:Erythrocytes / Q000737:chemistry; D006801:Humans; D013696:Temperature; D013997:Time Factors
D000255:Adenosine Triphosphate