Sequencing the maize genome.
Sequencing of complex genomes can be accomplished by enriching shotgun libraries for genes. In maize, gene-enrichment by copy-number normalization (high C(0)t) and methylation filtration (MF) have been used to generate up to two-fold coverage of the gene-space with less than 1 million sequencing reads. Simulations using sequenced bacterial artificial chromosome (BAC) clones predict that 5x coverage of gene-rich regions, accompanied by less than 1x coverage of subclones from BAC contigs, will generate high-quality mapped sequence that meets the needs of geneticists while accommodating unusually high levels of structural polymorphism. By sequencing several inbred strains, we propose a strategy for capturing this polymorphism to investigate hybrid vigor or heterosis.
Martienssen
Robert A
RA
Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA. martiens@cshl.org
Rabinowicz
Pablo D
PD
O'Shaughnessy
Andrew
A
McCombie
W Richard
WR
D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D018745:Genome, Plant; D020161:Physical Chromosome Mapping / Q000379:methods; D003313:Zea mays / Q000235:genetics*