[AFLP fingerprinting map analysis of Pleurotus ostreatus].
AFLP analysis was carried out with 14 Pleurotus ostreatus strains from different areas. Optimal conditions of the AFLP fingerprinting analysis for P. ostreatus were first tested and the results showed that the primer pairs E-3/M-3 especially E-AGC/M-CAT and E-AGC/M-ACC could give more amplified DNA fragments than others like E-2/M-1 or E-2/M-3. From the fingerprinting map of the primer pair E-AGC/M-CAT, 184 clear and stable DNA bands were observed, including 101 polymorphous bands that are accounted for 54.89%. The genetic similarity coefficient and genetic distance were calculated from AFLP data among 14 P. ostreatus strains. The genetic distance between these strains ranged from 0.192 to 0.754, indicating that P. ostreatus was rich in genetic diversity. UPGMA cluster analysis was also performed. It's shown that 14 P. ostreatus strains are divided into six groups, and strains from same areas or with similar best-growth temperature usually have higher comparability in the UPGMA tree. P. ostreatus P17 and P. ostreatus Za3, P. ostreatus Min31 and P. ostreatus Yiping have intimate genetic relationships with each other respectively which were consistent with its geographical distribution and best growth temperature. P. ostreatus Ce5 showed remarkable genetic differentiation, which has farther genetic relationships compared with other P. ostreatus strains. In addition, the reasons for cluster results of strains from AFLP data consistent with morphology, geographical distribution and optimal condition of AFLP fingerprinting analysis for P. ostreatus were discussed.
Meng
Yu
Y
Sichuan Key Laboratory of Molecular Biology & Biotechnology, College of Life Science, Sichuan University, Chengdu 610064, China.
Jiang
Chang-Shun
CS
Liao
Wen-Tao
WT
Zhang
Yi-Zheng
YZ
D016172:DNA Fingerprinting / Q000379:methods*; D020076:Pleurotus / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length