Legionella effectors that promote nonlytic release from protozoa.
Legionella pneumophila, the bacterial agent of legionnaires' disease, replicates intracellularly within a specialized vacuole of mammalian and protozoan host cells. Little is known about the specialized vacuole except that the Icm/Dot type IV secretion system is essential for its formation and maintenance. The Legionella genome database contains two open reading frames encoding polypeptides (LepA and LepB) with predicted coiled-coil regions and weak homology to SNAREs; these are delivered to host cells by an Icm/Dot-dependent mechanism. Analysis of mutant strains suggests that the Lep proteins may enable the Legionella to commandeer a protozoan exocytic pathway for dissemination of the pathogen.
Department of Microbiology, College of Physicians and Surgeons, Columbia University, 701 West 168th Street, New York, NY 10032, USA.
John
J
Chen
Karim Suwwan
KS
de Felipe
Margaret
M
Clarke
Hao
H
Lu
O Roger
OR
Anderson
Gil
G
Segal
Howard A
HA
Shuman
D000048:Acanthamoeba / Q000382:microbiology* / Q000502:physiology / Q000648:ultrastructure; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002460:Cell Line; D015169:Colony Count, Microbial; D000242:Cyclic AMP / Q000378:metabolism; D004023:Dictyostelium / Q000382:microbiology* / Q000502:physiology / Q000648:ultrastructure; D005089:Exocytosis; D016680:Genome, Bacterial; D006801:Humans; D016952:Legionella pneumophila / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*; D008247:Lysosomes / Q000502:physiology; D008264:Macrophages / Q000382:microbiology / Q000648:ultrastructure; D009154:Mutation; D016366:Open Reading Frames; D010588:Phagosomes / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014617:Vacuoles / Q000382:microbiology
D001426:Bacterial Proteins; C482638:LepA protein, Legionella pneumophila; C482639:LepB protein, Legionella pneumophila; D011993:Recombinant Fusion Proteins; D000242:Cyclic AMP