Mechanosensing and signal transduction in tendrils.
The perception of thigmic stimuli is a widespread phenomenon among plants with decisive meaning for the ability to survive. Beside a general sensitivity for mechanical stimuli many plants have evolved specialized organs with highly developed mechanisms to perceive and transduce the applied forces. Tendrils of Bryonia dioica and Pisum sativum have been chosen to study the effects of mechanical stimulation on plant physiology. Both types of tendrils, although exhibiting different morphology, respond to such a stimulus with a rapid coiling response to the dorsal side of the organ within minutes. The actual perception of the stimulus is most likely coupled to the cytoskeleton serving as the mediator between the physical stimulus and the biochemical response. Drugs affecting the status of the cytoskeleton were used to get more insights into this specific process. The results indicate that microtubuli (MT) play the most important role in the perception of thigmic stimuli in tendrils. Colchicine-mediated disruption of MT lead to total inhibition of the response to the thigmic stimulus in tendrils of Pisum and to a reduced response in Bryonia. Alamethicin, an ionophore that can mimic action potentials in membranes, was able to bypass this inhibition suggesting a direct involvement of MT in depolarization of the membranes. Auxin, however, which is also supposed to be involved in the regulation of the coiling response, failed to bypass colchicine-dependent inhibition. Vinblastine, another microtubule depolimerizing agent, did induce tendril coiling in Pisum without further stimulation. Application of taxol and other MT-stabilizing drugs as well as disruption of the actin network did not affect the coiling response of tendrils. In Pisum indole-3-acetic acid (IAA) is induced after mechanical stimulation during the coiling response, but not jasmonic acid. A further consequence of mechanical stimulation is the induction of an oxidative burst and an increase in soluble sugar. A model is presented integrating these results and might serve as a common basis for the understanding of the perception of mechanical stimuli.
Max Planck Institute of Chemical Ecology, Department of Bioorganics, Jena, Germany. jengelberth@gainesville.usda.ufl.edu
Jürgen
J
Engelberth
D000408:Alamethicin / Q000494:pharmacology; D029772:Bryonia / Q000502:physiology; D002473:Cell Wall / Q000502:physiology; D003078:Colchicine / Q000494:pharmacology; D003517:Cyclopentanes / Q000378:metabolism; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects; D007210:Indoleacetic Acids / Q000378:metabolism; D040542:Mechanotransduction, Cellular / Q000502:physiology; D008870:Microtubules / Q000187:drug effects / Q000502:physiology*; D009243:NAD / Q000378:metabolism; D019255:NADPH Oxidases / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism; D017239:Paclitaxel / Q000494:pharmacology; D018532:Pisum sativum / Q000502:physiology; D035261:Plant Components, Aerial / Q000187:drug effects* / Q000502:physiology*; D010937:Plant Growth Regulators / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology*; D013314:Stress, Mechanical; D050257:Tubulin Modulators / Q000494:pharmacology; D014475:Uncoupling Agents / Q000494:pharmacology; D014747:Vinblastine / Q000494:pharmacology
D003517:Cyclopentanes; D007210:Indoleacetic Acids; D054883:Oxylipins; D010937:Plant Growth Regulators; D050257:Tubulin Modulators; D014475:Uncoupling Agents; D009243:NAD; D015638:Cytochalasin D; D000408:Alamethicin; D014747:Vinblastine; C011006:jasmonic acid; C030737:indoleacetic acid; D019255:NADPH Oxidases; D017239:Paclitaxel; D003078:Colchicine