Development of a diagnostic PCR assay targeting the Mn-dependent superoxide dismutase gene (sodA) for identification of Streptococcus gallolyticus.
A PCR-based assay to identify Streptococcus gallolyticus has been developed. The assay uses an oligonucleotide primer pair targeting a partial sequence of the manganese-dependent superoxide dismutase gene (sodA). The assay distinguished members of the S. gallolyticus group from other, closely related taxa successfully by yielding a 408-bp specific amplicon.
Sasaki
Eiki
E
Department of Bioscience, Graduate School of Science and Technology, Kobe University, Nada-ku, Kobe 657-8501, Japan.
Osawa
Ro
R
Nishitani
Yosuke
Y
Whiley
Robert A
RA
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D005243:Feces / Q000382:microbiology; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D013290:Streptococcal Infections / Q000175:diagnosis*; D013291:Streptococcus / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D013482:Superoxide Dismutase / Q000235:genetics*
D001426:Bacterial Proteins; C095500:SodA protein, Bacteria; D013482:Superoxide Dismutase