Screening for oestrogenic activity of plant and food extracts using in vitro trout hepatocyte cultures.
The use of in vitro trout hepatocyte cultures is shown to provide a simple and effective way to screen plant and food products for oestrogenic activity. The relative oestrogenic activities of 0.1 g each of extracts of phytosterol, soy isoflavone, red clover, kudzu and soybean extracts were determined using this assay and found to be equivalent to 212, 1, 3.2, 132 and 1025 nM of 17beta-estradiol, respectively. Controls were performed on soybean and kudzu extracts using specific ELISAs for isoflavones and these confirmed the validity of the cell culture assay. The method described offers an advantage over current methods in that it can detect increased oestrogenic activity that may occur as a result of metabolic activation of pre- or pro-oestrogens liver cells.
Micronutriments, Reproduction and Health Unit, ENITA de Bordeaux, 1 cours du General de Gaulle, 33175 Gradignan, France. c-bennetau@enitab.fr
C
C
Bennetau-Pelissero
K Gontier
KG
Latonnelle
V
V
Lamothe
S
S
Shinkaruk-Poix
S J
SJ
Kaushik
D000818:Animals; D002478:Cells, Cultured; D031885:Cimicifuga / Q000737:chemistry; D028001:Eleutherococcus / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D022781:Hepatocytes / Q000187:drug effects*; D007529:Isoflavones / Q000302:isolation & purification* / Q000494:pharmacology*; D017686:Oncorhynchus mykiss; D048789:Phytoestrogens; D010936:Plant Extracts / Q000302:isolation & purification* / Q000494:pharmacology*; D028321:Plant Preparations / Q000302:isolation & purification* / Q000494:pharmacology*; D029908:Pueraria / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry; D029921:Trifolium / Q000737:chemistry; D014819:Vitellogenins / Q000032:analysis
D007529:Isoflavones; D048789:Phytoestrogens; D010936:Plant Extracts; D028321:Plant Preparations; D014819:Vitellogenins