L-alanine auxotrophy of Lactobacillus johnsonii as demonstrated by physiological, genomic, and gene complementation approaches.
Using a chemically defined medium without L-alanine, Lactobacillus johnsonii was demonstrated to be strictly auxotrophic for that amino acid. A comparative genetic analysis showed that all known genes involved in L-alanine biosynthesis are absent from the genome of L. johnsonii. This auxotrophy was complemented by heterologous expression of the Bacillus subtilis L-alanine dehydrogenase.
van der Kaaij
Hengameh
H
Nestlé Research Centre, Nestec, Ltd., CH-1000 Lausanne 26, Switzerland.
Desiere
Frank
F
Mollet
Beat
B
Germond
Jacques-Edouard
JE
D000409:Alanine / Q000378:metabolism*; D050788:Alanine Dehydrogenase; D000594:Amino Acid Oxidoreductases / Q000235:genetics / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005816:Genetic Complementation Test; D016680:Genome, Bacterial; D007778:Lactobacillus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*
D004269:DNA, Bacterial; D000594:Amino Acid Oxidoreductases; D050788:Alanine Dehydrogenase; D000409:Alanine