Preferential presence of decorin-binding protein B (BBA25) and BBA50 antibodies in cerebrospinal fluid of patients with neurologic Lyme disease.
Borrelia burgdorferi antibodies preferentially present in cerebrospinal fluid (CSF) were examined by differentially probing a B. burgdorferi expression library with CSF and sera from patients with neurologic Lyme disease. Several phage clones selectively reacted with CSF, and these genes were then expressed in recombinant form and used to detect specific antibody in an enzyme-linked immunosorbent assay. Decorin-binding protein B (BBA25) and BBA50 (hypothetical protein) elicited immunoglobulin G (IgG) or IgM detectable in CSF-but not sera-of patients, demonstrating preferential antibody production during neuroborreliosis.
Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, USA. erol.fikrig@yale.edu
Erol
E
Fikrig
Patricia K
PK
Coyle
Steven E
SE
Schutzer
Manchuan
M
Chen
Zhidian
Z
Deng
Richard A
RA
Flavell
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial / Q000134:cerebrospinal fluid*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000032:analysis; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000276:immunology*; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology*; D002352:Carrier Proteins / Q000032:analysis* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D020852:Lyme Neuroborreliosis / Q000134:cerebrospinal fluid*
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; C112557:DdpB protein, Borrelia burgdorferi; D007074:Immunoglobulin G; D007075:Immunoglobulin M