Determination of hypericin content in Hypericum triquetrifolium Turra (Hypericaceae) growing wild in Jordan.
Hypericin content of methanolic extracts of dried flowers, leaves, stems, and roots of Hypericum triquetrifolium (Turra) were determined by HPLC. Conversion of protohypericin to hypericin was achieved by exposing samples to light for 30 min immediately before HPLC analysis. External standard calibration was used to quantify hypericin. Leaves showed the highest hypericin content of 0.36% w/w. Total aerial parts contained 0.43% w/w of hypericin. The relatively high hypericin content of the H. triquetrifolium in this study encourages the introduction, cultivation, and biological evaluation of hypericum specie in Jordan.
Alali
Feras
F
Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan. Falali@just.edu.jo
Tawaha
Khaled
K
Al-Eleimat
Tamam
T
D000873:Anthracenes; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000032:analysis*; D020902:Hypericum / Q000737:chemistry*; D007597:Jordan; D010569:Perylene / Q000031:analogs & derivatives* / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis
D000873:Anthracenes; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D010569:Perylene; C004965:hypericin