[The study of dendritic cells derived from acute myeloid leukemia cells].
OBJECTIVE
To induce dendritic cells (DCs) from primary acute myeloid leukemia (AML) cells with cytokines, so as to provide a new approach for immunotherapy of leukemia.


METHODS
Bone marrow MNCs were isolated from 12 AML patients and 6 healthy donors, and co-cultured with rhGM-CSF 1000 U/ml, rhIL-4500 U/ml and TNF-alpha 50 U/ml for 10 days. The morphologic features were observed by Wright's staining, inverted microscope and electron microscope. CD(80), CD(86), CD(83), CD(1a), HLA-DR expression were assayed by flow cytometry, cytogenetic analysis was performed by fluorescence in-situ hybridization (FISH), the function of antigen presenting were tested by mixed lymphocyte reaction (MLR).


RESULTS
After cultured with cytokines, typical dendritic appearance with delicate membrane projections could be founded, the CD(80), CD(86), CD(83), CD(1a) markers and capacity of stimulating allogeneic T cells were upregulated significantly. The cultured DCs were confirmed to generate from malignant origin. There have no difference in morphology and immunophenotype expression between the DCs from AML patients and those from normal individuals. However, DCs derived from AML patients displayed decreased activity when tested in MLR.


CONCLUSIONS
Primary AML cells could be induced into AML-DCs with cytokines. The study suggests that it may be used efficiently in immunotherapy of AML.
Department of Hematology, The First Affiliated Hospital, Zhejiang University Medical College, Hangzhou 310003, China.
Xiang-min
XM
Tong
Jie
J
Jin
Yong-quan
YQ
Xue
Yun-gui
YG
Wang
D000208:Acute Disease; D015703:Antigens, CD / Q000032:analysis*; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology / Q000648:ultrastructure; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000648:ultrastructure; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007951:Leukemia, Myeloid / Q000097:blood* / Q000473:pathology / Q000628:therapy; D008854:Microscopy, Electron; D020133:Reverse Transcriptase Polymerase Chain Reaction
D015703:Antigens, CD