PCR for varicella zoster virus genome negative in corneal epithelial cells of patients with Thygeson's superficial punctate keratitis.
OBJECTIVE
To find out if varicella zoster virus is the causative agent of Thygeson's superficial punctuate keratitis.


METHODS
Epithelial cells were harvested from the punctuate epithelial lesions 9 patients with Thygeson's superficial punctate keratitis. After DNA extraction polymerase chain reaction was carried out with varicella zoster virus primers.


RESULTS
All samples were negative with regard to varicella zoster virus genome.


CONCLUSIONS
This result suggests that varicella zoster virus is most probably not the causative agent of Thygeson's superficial punctate keratitis.
Eye Hospital, Heinrich-Heine University, Düsseldorf, Germany. thomas.reinhard@uni-duesseldorf.de
T
T
Reinhard
M
M
Roggendorf
I
I
Fengler
R
R
Sundmacher
D000293:Adolescent; D000328:Adult; D002648:Child; D003315:Cornea / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D004847:Epithelial Cells / Q000821:virology; D005260:Female; D006563:Herpes Zoster Ophthalmicus / Q000150:complications*; D014645:Herpesvirus 3, Human / Q000302:isolation & purification*; D006801:Humans; D007634:Keratitis / Q000821:virology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods
D004279:DNA, Viral