Effects of conjugated linoleic acid on body fat accumulation and serum lipids in hamsters fed an atherogenic diet.
Conjugated linoleic acid (CLA) refers to a mixture of naturally occurring positional and geometric isomers of linoleic acid that exist in dairy products and meat. The aim of the present work was to study the effects of c-9,t-11 and t-10,c-12 CLA isomers on body fat accumulation and serum lipids in hamsters fed an atherogenic diet. Hamsters were divided in four groups: one group was fed a chow diet (control) and the other three groups were given semi-purified atherogenic diets with 0.5% linoleic acid (LA), c-9,t-11 or t-10,c-12 CLA. Body weight and food intake were measured daily. After 6 weeks, adipose tissues from different anatomical locations and liver were dissected and weighed. Serum glucose, total cholesterol, HDL-c, LDL-c and triacylglycerol levels, as well as total and free cholesterol, triacylglycerol and phospholipid content in liver were determined by enzymatic methods. No differences in either energy intake or final body weight were found. The addition of t-10,c-12 CLA reduced fat accumulation and led to lower serum cholesterol, as compared with LA group. Nevertheless the level remained higher than in the control animals. The reduction in serum cholesterol was limited to LDL-c. This isomer also reduced triacylglycerol content in liver but did not modify serum triacylglycerol level. In summary, the present study demonstrates that t-10,c-12 CLA is the biologically active agent when anti-obesity and hypocholesterolaemic properties of CLA are considered. In contrast, the isomer c-9,t-11 has no effect on lipid metabolism in hamsters.
Department of Nutrition and Food Science, University of the Basque Country, Paseo de la Universidad 7, 01006 Vitoria, Spain.
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Navarro
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Zabala
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Macarulla
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Fernández-Quintela
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Rodríguez
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Simón
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Portillo
D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000032:analysis / Q000187:drug effects; D001823:Body Composition / Q000187:drug effects*; D001835:Body Weight / Q000187:drug effects; D002784:Cholesterol / Q000097:blood / Q000737:chemistry; D006224:Cricetinae; D004036:Diet, Atherogenic; D004435:Eating / Q000187:drug effects; D002149:Energy Intake / Q000187:drug effects; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D008055:Lipids / Q000097:blood*; D008099:Liver / Q000378:metabolism; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010743:Phospholipids / Q000737:chemistry; D014280:Triglycerides / Q000097:blood / Q000737:chemistry
D001786:Blood Glucose; D044243:Linoleic Acids, Conjugated; D008055:Lipids; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol