Analysis of digenic epistatic effects and QE interaction effects QTL controlling grain weight in rice.
Immortalized F(2) population of rice (Oryza sativa L.) was developed by randomly mating F(1) among recombinant inbred (RI) lines derived from (Zhenshan 97B x Minghui 63), which allowed replications within and across environments. QTL (quantitative trait loci) mapping analysis on kilo-grain weight of immortalized F(2) population was performed by using newly developed software for QTL mapping, QTLMapper 2.0. Eleven distinctly digenic epistatic loci included a total of 15 QTL were located on eight chromosomes. QTL main effects of additive, dominance, and additive x additive, additive x dominance, and dominance x dominance interactions were estimated. Interaction effects between QTL main effects and environments (QE) were predicted. Less than 40% of single effects, most of which were additive effects, for identified QTL were significant at 5% level. The directional difference for QTL main effects suggested that these QTL were distributed in parents in the repulsion phase. This should make it feasible to improve kilo-grain weight of both parents by selecting appropriate new recombinants. Only few of the QE interaction effects were significant. Application prospect for QTL mapping achievements in genetic breeding was discussed.
Gao
Yong-ming
YM
Agronomy Department, Zhejiang University, Hangzhou 310029, China.
Zhu
Jun
J
Song
You-shen
YS
He
Ci-xin
CX
Shi
Chun-hai
CH
Xing
Yong-zhong
YZ
D032461:Chromosomes, Plant / Q000235:genetics; D003433:Crosses, Genetic; D004777:Environment; D004843:Epistasis, Genetic; D012275:Oryza / Q000033:anatomy & histology* / Q000235:genetics*; D010641:Phenotype; D040641:Quantitative Trait Loci