Preformulation stability of Spantide II, a promising topical anti-inflammatory agent for the treatment of psoriasis and contact dermatitis.
Substance P is readily expressed in skin inflammatory disorders such as psoriasis and contact dermatitis. Spantide II is a peptide (MW 1668.76) that specifically binds to neurokinin-1 receptor (NKR-1) and blocks inflammation associated with substance P. The anti-inflammatory property of Spantide II makes it a suitable candidate to be studied as a topical formulation for the treatment of dermal inflammatory disorders. The objective of this study was to investigate the influence of pH, temperature, salt concentration and concentration on the aqueous stability of Spantide II. The stability of Spantide II was also assessed by circular dichroic (CD) spectroscopy and mass spectrometry (MS). The influence of various dermatological vehicles (ethanol, Transcutol, propylene glycol, N-methyl-2-pyrrolidone (NMP), ethyl oleate, isopropyl myristate and laurogylcol FCC (LFCC)) on the stability of Spantide II was investigated. A precise high-performance liquid chromatography (HPLC) assay was developed for analysis of Spantide II. At higher temperature (40 degrees C) the stability of Spantide II decreased with increase in pH (P < 0.05). Change in salt concentration did not appreciably affect the stability of Spantide II (P > 0.05). The concentration of Spantide II in the solution had no significant influence on its stability (P > 0.05). CD spectroscopy studies showed that Spantide II has a relatively stable alpha-helix structure in the liquid state. The stability of Spantide II was affected by the type of vehicle used in the study (P < 0.01) at different temperatures (P < 0.05). Spantide II at high temperature undergoes lysine-proline diketopiperazine degradation as evident in MS data. Spantide II was relatively more stable in ethyl oleate-ethanol, ethanol-water, ethanol and N-methyl-2-pyrrolidone. The results of this study indicate that ethyl oleate-ethanol (1:1) and ethanol-water (1:1) could be used as potential vehicles in the development of topical formulations of Spantide II.
Division of Pharmaceutics, College of Pharmacy and Pharmaceutical Sciences, Florida A and M University, Tallahassee, Florida, USA.
Loice
L
Kikwai
R J
RJ
Babu
Narayanasamy
N
Kanikkannan
Mandip
M
Singh
D000287:Administration, Topical; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D003877:Dermatitis, Contact / Q000188:drug therapy; D004355:Drug Stability; D004356:Drug Storage; D006863:Hydrogen-Ion Concentration; D014677:Pharmaceutical Vehicles; D011565:Psoriasis / Q000188:drug therapy; D012965:Sodium Chloride / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013373:Substance P / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000627:therapeutic use; D013696:Temperature
D000893:Anti-Inflammatory Agents; D014677:Pharmaceutical Vehicles; C065446:spantide II; D013373:Substance P; D012965:Sodium Chloride