Symbiont-induced changes in host actin during the onset of a beneficial animal-bacterial association.
The influence of bacteria on the cytoskeleton of animal cells has been studied extensively only in pathogenic associations. We characterized changes in host cytoskeletal actin induced by the bacterial partner during the onset of a cooperative animal-bacteria association using the squid-vibrio model. Two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis revealed that Vibrio fischeri induced a dramatic increase in actin protein abundance in the bacteria-associated host tissues during the onset of the symbiosis. Immunocytochemistry revealed that this change in actin abundance correlated with a two- to threefold increase in actin in the apical cell surface of the epithelium-lined ducts, the route of entry of symbionts into host tissues. Real-time reverse transcriptase PCR and in situ hybridization did not detect corresponding changes in actin mRNA. Temporally correlated with the bacteria-induced changes in actin levels was a two- to threefold decrease in duct circumference, a 20% loss in the average number of cells interfacing with the duct lumina, and dramatic changes in duct cell shape. When considered with previous studies of the biomechanical and biochemical characteristics of the duct, these findings suggest that the bacterial symbionts, upon colonizing the host organ, induce modifications that physically and chemically limit the opportunity for subsequent colonizers to pass through the ducts. Continued study of the squid-vibrio system will allow further comparisons of the mechanisms by which pathogenic and cooperative bacteria influence cytoskeleton dynamics in host cells.
Pacific Biomedical Research Center, Kewalo Marine Laboratory, University of Hawaii, Honolulu, Hawaii 96813, USA.
Jennifer R
JR
Kimbell
Margaret J
MJ
McFall-Ngai
D000199:Actins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D003599:Cytoskeleton / Q000378:metabolism; D049832:Decapodiformes / Q000033:anatomy & histology / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D004848:Epithelium / Q000378:metabolism; D015870:Gene Expression; D017403:In Situ Hybridization; D008954:Models, Biological; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013559:Symbiosis / Q000235:genetics / Q000502:physiology*; D014733:Vibrio / Q000378:metabolism*
D000199:Actins; D012333:RNA, Messenger