Biological diagnosis of primary intraocular lymphoma.
Primary intraocular lymphoma (PIOL) is a rare presentation of lymphoma that is particularly difficult to recognize. In our institution, 36 cases of PIOL were diagnosed between March 1997 and July 2002. The recognition of lymphoma cells by cytology with or without immunophenotyping on slides generated a strong suspicion of the diagnosis in 34 of 36 cases. The diagnosis was confirmed by measurement of interleukin-10 (IL-10) in the vitreous humour or aqueous humour; high levels were observed in 35 of 36 cases, all were of B-cell origin. As expected, the only case with T-cell lymphoma had a very low level of IL-10. Furthermore, IL-10 levels excluded this diagnosis in two cases that were incorrectly suspected of PIOL after cytological examination. Finally, detection of clonality by polymerase chain reaction techniques, performed in 29 cases, represented a helpful tool in diagnosing PIOL as this approach definitively confirmed the diagnosis of B- or T-cell lymphoma in 17 cases.
Service d'Hématologie Biologique Service d'Ophthalmologie, Hôpital Pitié-Salpêtrière, Paris, France.
Hélène
H
Merle-Béral
Frédéric
F
Davi
Nathalie
N
Cassoux
Sylvie
S
Baudet
Chantal
C
Colin
Thomas
T
Gourdet
Bahram
B
Bodaghi
Phuc
P
LeHoang
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000032:analysis; D005134:Eye Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology; D005260:Female; D015321:Gene Rearrangement; D006801:Humans; D007124:Immunoenzyme Techniques; D016130:Immunophenotyping; D016753:Interleukin-10 / Q000032:analysis; D016393:Lymphoma, B-Cell / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology; D016399:Lymphoma, T-Cell / Q000175:diagnosis / Q000235:genetics / Q000276:immunology; D008297:Male; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000473:pathology; D014822:Vitreous Body / Q000276:immunology
D015415:Biomarkers; D016753:Interleukin-10