Regulation of human T-cell leukemia virus type 1 (HTLV-1) budding by ubiquitin ligase Nedd4.
The Gag protein of human T-cell leukemia virus type 1 (HTLV-1) contains the conserved sequences PPxY and PTAP, which are putative viral motifs required for budding (L-domain motifs). We show here that the PPxY motif, but not the PTAP motif, is essential for HTLV-1 virion budding from the plasma membrane. In addition, we show that overexpression of Nedd4 enhances HTLV-1 budding and that Nedd4 interacts with Gag via its WW domain. The HECT domain of Nedd4 is also required for budding. These results indicate that Nedd4 or a Nedd4-related ubiquitin ligase plays a critical role in HTLV-1 budding.
Division of Molecular Virology, Institute for Genetic Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo 060-0815, Japan.
Akira
A
Sakurai
Jiro
J
Yasuda
Hiroko
H
Takano
Yuetsu
Y
Tanaka
Masanori
M
Hatakeyama
Hisatoshi
H
Shida
D020816:Amino Acid Motifs; D056827:Endosomal Sorting Complexes Required for Transport; D015683:Gene Products, gag / Q000737:chemistry* / Q000502:physiology; D006367:HeLa Cells; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D000075702:Nedd4 Ubiquitin Protein Ligases; D044767:Ubiquitin-Protein Ligases / Q000737:chemistry / Q000502:physiology*
D056827:Endosomal Sorting Complexes Required for Transport; D015683:Gene Products, gag; D000075702:Nedd4 Ubiquitin Protein Ligases; C000618483:Nedd4 protein, human; D044767:Ubiquitin-Protein Ligases