Crystallization and preliminary X-ray analysis of GlcNAc alpha 1,4Gal-releasing endo-beta-galactosidase from Clostridium perfringens.
The unique clostridial endo-beta-galactosidase (Endo-beta-Gal(GnGa)) capable of releasing the disaccharide GlcNAc alpha 1,4Gal from O-glycans expressed in the gastric gland mucous cell-type mucin has been crystallized. The crystal belongs to space group P6(3), with unit-cell parameters a = 160.4, c = 86.1 A. Under cryocooled conditions and using a synchrotron X-ray source, the crystals diffract to 1.82 A resolution. The asymmetric unit contains two or three molecules.
Department of Chemistry, University of Georgia, Athens, GA 30602, USA.
Lu
L
Deng
Zhi-Jie
ZJ
Liu
Hisashi
H
Ashida
Su-Chen
SC
Li
Yu-Teh
YT
Li
Peter
P
Horanyi
Wolfram
W
Tempel
John
J
Rose
Bi-Cheng
BC
Wang
D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D003016:Clostridium perfringens / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004187:Disaccharides / Q000378:metabolism*; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D009077:Mucins / Q000378:metabolism; D012795:Sialoglycoproteins / Q000378:metabolism
D001426:Bacterial Proteins; D004187:Disaccharides; D009077:Mucins; D012795:Sialoglycoproteins; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase