[Experimental study on in vitro lamina propria engineering using oral fibroblast and polyglycolic acids].
OBJECTIVE
This study investigated the feasibility of lamina propria engineering in vitro using expanded oral fibroblast(OFC) and Polyglycolic Acids (PGA).


METHODS
OFC were isolated by tissue explant method and expanded in vitro. OFC (20x10(6)) of 3rd passage were collected and then seeded onto PGA unwoven fibers to form a cell-scaffold. The constructs were cultured in DMEM +10% FBS. The cell-scaffold constructs were observed continuously by microscope. Small fragments were harvested at 1 week for electromicroscope, histological and RT-PCR analysis.


RESULTS
At the sixth day, a neo-lamina propria was formed. HE and Masson stain revealed the formation of collagen fibers. RT-PCR revealed the new forming collagen was mainly type I collagen.


CONCLUSIONS
lamina propria tissue is possible to engineer in vitro using oral fibroblast and polyglycolic acids. At this basis, we can construct bi-layer tissue engineering oral mucosa in the further research.
Zhou
Zeng-tong
ZT
Department of Oral Medicine, School of Stomatology,Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China. zhouzengtong@hotmail.com
Huang
He
H
Li
Qing
Q
Cao
De-jun
DJ
Liu
Wei
W
Cao
Yi-lin
YL
D005347:Fibroblasts / Q000166:cytology / Q000648:ultrastructure; D006801:Humans; D008854:Microscopy, Electron; D009061:Mouth Mucosa / Q000166:cytology* / Q000648:ultrastructure; D011100:Polyglycolic Acid / Q000494:pharmacology*; D023822:Tissue Engineering
D011100:Polyglycolic Acid