PrLZ, a novel prostate-specific and androgen-responsive gene of the TPD52 family, amplified in chromosome 8q21.1 and overexpressed in human prostate cancer.
We report a previously unrecognized prostate-specific protein, PrLZ (prostate leucine zipper), a new member of the Tumor Protein D52 (TPD52) family. The gene for PrLZ was localized at chromosome 8q21.1, a locus most frequently amplified in human prostate cancer. Multiple tissue analyses demonstrated PrLZ predominantly in the prostate gland. Although its expression was enhanced by androgens in androgen receptor-expressing cells, PrLZ was detected in all of the human prostate cancer cell lines, regardless of androgen receptor status. Monoclonal anti-PrLZ antibodies were produced and intense immunohistochemical staining of PrLZ was observed in prostate epithelial cells in intraepithelial neoplasia and prostate cancer, whereas lower-level staining was detected in normal and benign epithelial components of the prostate gland. As the only prostate-specific gene identified in the most frequently amplified genomic region in prostate cancer, PrLZ may be the link between chromosome 8q amplification and malignant transformation of the prostate epithelia.
Molecular Urology and Therapeutics, Department of Urology, Emory University School of Medicine, Atlanta, GA 30322, USA. rwang2@emory.edu
Ruoxiang
R
Wang
Jianchun
J
Xu
Outi
O
Saramäki
Tapio
T
Visakorpi
William M
WM
Sutherland
Jianguang
J
Zhou
Buer
B
Sen
So Dug
SD
Lim
Nicola
N
Mabjeesh
Mahul
M
Amin
Jin-Tang
JT
Dong
John A
JA
Petros
Peter S
PS
Nelson
Fray F
FF
Marshall
Haiyen E
HE
Zhau
Leland W K
LW
Chung
D000728:Androgens / Q000494:pharmacology*; D014408:Biomarkers, Tumor; D045744:Cell Line, Tumor; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D005784:Gene Amplification; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000235:genetics*
D000728:Androgens; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; C114751:TPD52 protein, human