Abiotic stress induces apoptotic-like features in tobacco that is inhibited by expression of human Bcl-2.
The shared features between plant and animal programmed cell death are becoming increasingly apparent. In this study, human Bcl-2, an anti-apoptotic member of the Bcl-2 family of cell death regulators, was stably expressed in tobacco. Previously, we have shown that such plants were resistant/tolerant to several necrotrophic fungal pathogens. In this study, we show that transgenic plants are protected by several lethal abiotic stresses including heat, cold, menadione and hydrogen peroxide. Importantly, wild type tobacco, exposed to these treatments, not only died but during the death process exhibited features associated with mammalian apoptosis including DNA laddering, fragmentation, and the development of apoptotic bodies. These features were not observed in viable transgenic tobacco. Thus, abiotic stress induced cell death in plants can be accompanied by apoptotic-like features that are inhibited by expression of Bcl-2. These observations add to the growing body of evidence indicating trans-kingdom conservation of programmed cell death mechanisms.
Department of Plant Pathology, 406 Plant Sciences Hall, University of Nebraska, Lincoln, NE 68583, USA.
Wei
W
Li
Martin B
MB
Dickman
D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D003080:Cold Temperature; D019254:Genes, bcl-2 / Q000235:genetics* / Q000502:physiology; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000633:toxicity; D018384:Oxidative Stress; D030821:Plants, Genetically Modified / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D014026:Nicotiana / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D024483:Vitamin K 3 / Q000633:toxicity
D024483:Vitamin K 3; D006861:Hydrogen Peroxide