Effects of periodontitis and smoking on the microbiota of oral mucous membranes and saliva in systemically healthy subjects.
OBJECTIVE
To examine the differences in the proportions of 40 bacterial species in samples from eight oral soft-tissue surfaces and saliva in systemically healthy adult subjects with and without periodontitis and in smokers and nonsmokers.


METHODS
Saliva and microbial samples were taken from eight oral soft-tissue surfaces in 229 systemically healthy subjects stratified by periodontal and smoking status. Subjects included 84 periodontally healthy and 145 periodontitis individuals. Of these, there were 182 nonsmokers and 47 smokers. Soft-tissue samples were taken using a "buccal brush" and saliva was taken by expectoration. All samples were individually evaluated for their content of 40 bacterial species using checkerboard DNA-DNA hybridization. The percentage of total DNA probe count was determined for each species at each sample location and averaged across subjects. The significance of differences among the proportions of the 40 test species at different sample locations was sought in these populations using the Kruskall-Wallis test and adjusted for multiple comparisons.


RESULTS
When stratified for periodontal status or smoking status, microbial profiles at most sites did not differ significantly. Generally, periodontal pathogens were found at higher levels on the soft tissues of periodontitis subjects than in periodontally healthy subjects, and at higher levels in smokers than nonsmokers. Few significant differences were found, although trends in the populations were noted.


CONCLUSIONS
Small differences in the proportions of bacterial species were observed on soft tissues and in saliva comparing periodontally healthy and periodontitis subjects. Somewhat greater differences between smokers and nonsmokers were found; however, these were not statistically significant.
Mager
Donna L
DL
Department of Periodontology, The Forsyth Institute, Boston, MA 02115, USA. dmager@forsyth.org
Haffajee
Anne D
AD
Socransky
Sigmund S
SS
D000190:Actinomyces / Q000145:classification; D000328:Adult; D001419:Bacteria / Q000145:classification* / Q000254:growth & development; D015169:Colony Count, Microbial; D003773:Dental Plaque / Q000382:microbiology; D005260:Female; D005673:Fusobacterium / Q000145:classification; D005884:Gingival Hemorrhage / Q000382:microbiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009060:Mouth Floor / Q000382:microbiology; D009061:Mouth Mucosa / Q000382:microbiology*; D017622:Periodontal Attachment Loss / Q000382:microbiology; D010514:Periodontal Pocket / Q000382:microbiology; D010518:Periodontitis / Q000382:microbiology*; D018720:Prevotella / Q000145:classification; D012463:Saliva / Q000382:microbiology*; D012907:Smoking; D018709:Statistics, Nonparametric; D014059:Tongue / Q000382:microbiology