A tiling resolution DNA microarray with complete coverage of the human genome.
We constructed a tiling resolution array consisting of 32,433 overlapping BAC clones covering the entire human genome. This increases our ability to identify genetic alterations and their boundaries throughout the genome in a single comparative genomic hybridization (CGH) experiment. At this tiling resolution, we identified minute DNA alterations not previously reported. These alterations include microamplifications and deletions containing oncogenes, tumor-suppressor genes and new genes that may be associated with multiple tumor types. Our findings show the need to move beyond conventional marker-based genome comparison approaches, that rely on inference of continuity between interval markers. Our submegabase resolution tiling set for array CGH (SMRT array) allows comprehensive assessment of genomic integrity and thereby the identification of new genes associated with disease.
Ishkanian
Adrian S
AS
British Columbia Cancer Research Centre, 601 West 10th Avenue, Vancouver, British Columbia V5Z 1L3, Canada.
Malloff
Chad A
CA
Watson
Spencer K
SK
DeLeeuw
Ronald J
RJ
Chi
Bryan
B
Coe
Bradley P
BP
Snijders
Antoine
A
Albertson
Donna G
DG
Pinkel
Daniel
D
Marra
Marco A
MA
Ling
Victor
V
MacAulay
Calum
C
Lam
Wan L
WL
D022202:Chromosomes, Artificial, Bacterial; D018628:Gene Dosage; D015894:Genome, Human; D006801:Humans; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured