Immunohistochemical demonstration of the small GTPase RhoA on epoxy-resin embedded sections.
The purpose of the present study was to establish a method for light microscopical immunohistochemical localization of the small G protein RhoA on specimens treated and embedded for routine transmission electron microscopy. There are advantages in antigen immunolocalization on resin semi-thin sections compared to cryostat or paraffin sections: the preservation of morphological details, the well-defined immunoprecipitate localization and the possibility to correlate the immunohistochemical results with those obtained by electron microscope on neighbouring sections. These advantages are particularly useful for the subcellular localization of low molecular weight proteins such as RhoA, a small G protein able to cycle from the inactive cytoplasmic form to the plasma membrane-bound active form.
Department of Human Morphology and Applied Biology, Section of Histology and Medical Embryology, University of Pisa, Via Roma 55, I-56126 Pisa, Italy.
D
D
D'Alessandro
L
L
Mattii
S
S
Moscato
N
N
Bernardini
C
C
Segnani
A
A
Dolfi
F
F
Bianchi
D000328:Adult; D000818:Animals; D004853:Epoxy Resins; D018922:HL-60 Cells; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D007668:Kidney / Q000201:enzymology / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron; D010179:Pancreas / Q000201:enzymology / Q000648:ultrastructure; D051381:Rats; D016610:Tissue Embedding / Q000379:methods; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*
D004853:Epoxy Resins; D020742:rhoA GTP-Binding Protein