Quantitative characterisation of the morphology of Trichoderma harzianum cultured in shake-flasks and containing tween 40.
Image analysis was used to measure the effect of Tween 40 on the morphology of Trichoderma harzianum. The percentage of pellets was maximal (93%) with Tween at 0.2 ml l(-1), whereas the maximal proportion of dispersed mycelia (40%) was with Tween at 1.6 ml l(-1). The particle median diameter decreased from 2 to 0.5 mm as Tween concentration was increased and was inversely correlated to the biomass dry weight. Adding pre-determined aliquots of Tween 40 to the culture medium can be used to define the morphological characteristics of Trichoderma harzianum in submerged shake-flask culture.
Instituto de Biotecnología-UNAM, Cuernavaca, Morelos, México.
Savidra
S
Lucatero
Enrique
E
Galindo
C Patricia
CP
Larralde-Corona
D019149:Bioreactors / Q000382:microbiology; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D025282:Mycelium / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development*; D011136:Polysorbates / Q000494:pharmacology*; D013172:Spores, Fungal / Q000166:cytology / Q000187:drug effects / Q000254:growth & development; D014242:Trichoderma / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development*
D011136:Polysorbates; C068430:polysorbate 40