Enrichment of extremely noisy high-throughput screening data using a naïve Bayes classifier.
The noise level of a high-throughput screening (HTS) experiment depends on various factors such as the quality and robustness of the assay itself and the quality of the robotic platform. Screening of compound mixtures is noisier than screening single compounds per well. A classification model based on naïve Bayes (NB) may be used to enrich such data. The authors studied the ability of the NB classifier to prioritize noisy primary HTS data of compound mixtures (5 compounds/well) in 4 campaigns in which the percentage of noise presumed to be inactive compounds ranged between 81% and 91%. The top 10% of the compounds suggested by the classifier captured between 26% and 45% of the active compounds. These results are reasonable and useful, considering the poor quality of the training set and the short computing time that is needed to build and deploy the classifier.
Glick
Meir
M
Novartis Institute for Biomedical Research, Cambridge, MA 02139, USA. meir.glick@pharma.novartis.com
Klon
Anthony E
AE
Acklin
Pierre
P
Davies
John W
JW
D001499:Bayes Theorem; D010600:Pharmacology; D012371:Robotics