Arginine administration decreases cerebral cortex acetylcholinesterase and serum butyrylcholinesterase probably by oxidative stress induction.
In the present study we investigated the action of vitamins E and C on the inhibition of acetylcholinesterase and butyrylcholinesterase activities provoked by arginine in cerebral cortex and serum of 60-day-old rats. Animals were pretreated for 1 week with daily intraperitoneal administration of saline (control) or vitamins E (40 mg/kg) and C (100 mg/kg). Twelve hours after the last injection, animals received one injection of arginine (0.8 microM/g of body weight) or saline. Results showed that acetylcholinesterase and butyrylcholinesterase activities were decreased in the arginine-treated rats. Furthermore, pretreatment with vitamins E and C prevented these effects. The data indicate that the reduction of acetylcholinesterase and butyrylcholinesterase activities caused by arginine was probably mediated by oxidative stress. Assuming the possibility that these effects might also occur in the human condition, our findings may be relevant to explain, at least in part, the neurological dysfunction associated with hyperargininemia and might support a novel therapeutic strategy to slow the progression of neurodegeneration in this disorder.
Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, Porto Alegre, RS, Brazil. wyse@ufrgs.br
Angela T S
AT
Wyse
Francieli M
FM
Stefanello
Fábria
F
Chiarani
Débora
D
Delwing
Clóvis M D
CM
Wannmacher
Moacir
M
Wajner
D000110:Acetylcholinesterase / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D001120:Arginine / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000008:administration & dosage; D002091:Butyrylcholinesterase / Q000097:blood* / Q000187:drug effects; D002540:Cerebral Cortex / Q000201:enzymology*; D007274:Injections, Intraperitoneal; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000008:administration & dosage
D000975:Antioxidants; D014810:Vitamin E; D001120:Arginine; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D001205:Ascorbic Acid