Synthesis and antisense properties of oligodeoxyribonucleotides containing C5-substituted arabinofuranosyluracil.
An oligodeoxyribonucleotide (ODN) containing three C5-substituted arabinofuranosyluracils was synthesized by the post-synthetic modification method from the ODN containing three C5-substituted 2,2'-anhydrouridines. The stability of the modified ODN/DNA duplex was lower than that of the corresponding normal duplex but that of the modified ODN/RNA duplex showed little change. The modified ODN could induce RNase H activity and was resistant against nuclease.
Ozaki
Hiroaki
H
Department of Chemistry, Faculty of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma 376-8515, Japan. ozaki@chem.gunma-u.ac.jp
Nakajima
Kiyohiro
K
Kuwahara
Masayasu
M
Sawai
Hiroaki
H
D001086:Arabinofuranosyluracil / Q000138:chemical synthesis* / Q000235:genetics*; D020319:Oligodeoxyribonucleotides, Antisense / Q000138:chemical synthesis* / Q000235:genetics*
D020319:Oligodeoxyribonucleotides, Antisense; D001086:Arabinofuranosyluracil