Peptides as modulators of enzymes and regulatory proteins.
There is currently great interest in the development of methods to modulate the function of diverse classes of target proteins with chemicals (agonists or antagonists). These would be valuable reagents for biomedical research and some might serve as potential drug leads. Traditionally, most chemicals that modulate protein function have been enzyme inhibitors isolated in functional screens specific for the enzyme of interest. However, recent efforts from many laboratories have suggested that relatively simple binding assays may provide a more convenient and general route to chemical modulators. We review here this work with a particular emphasis on peptide modulators.
Department of Internal Medicine and Molecular Biology, Center for Biomedical Inventions, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390-8573, USA.
Larisa A
LA
Troitskaya
Thomas
T
Kodadek
D042846:CDC2-CDC28 Kinases / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism; D020650:Combinatorial Chemistry Techniques; D051357:Cyclin-Dependent Kinase 2; D020536:Enzyme Activators / Q000138:chemical synthesis / Q000302:isolation & purification / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000302:isolation & purification / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015942:Factor VIIa / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008956:Models, Chemical; D010452:Peptide Biosynthesis / Q000235:genetics; D019151:Peptide Library; D010455:Peptides / Q000138:chemical synthesis / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D033921:Vesicular Transport Proteins
C066503:CDC6 protein, S cerevisiae; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D020536:Enzyme Activators; D004791:Enzyme Inhibitors; D004798:Enzymes; C055002:GAL80 protein, S cerevisiae; C089990:PEP8 protein, S cerevisiae; D019151:Peptide Library; D010455:Peptides; D011506:Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D033921:Vesicular Transport Proteins; D007770:L-Lactate Dehydrogenase; D042846:CDC2-CDC28 Kinases; D051357:Cyclin-Dependent Kinase 2; D015942:Factor VIIa