[Identification of differential expression ESTs in pig muscle tissue by DHPLC].
The differential expression ESTs in muscle tissue of Large Yorkshire pig and Lantang pig, one of the local pig breeds in Guangdong province, were identified with DHPLC system and graphic analysis software LabWork4.0, and both methods verified the true differential display bands. The results showed that DHPLC can quickly and accurately detect differential expression mRNA profiles of different tissues, and can be widely used to study mRNA expression, differential expression of different animal tissues under variant environments.
College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Chong
C
Wang
Yao-Sheng
YS
Chen
Chong-Sheng
CS
Li
Zhi-Hong
ZH
Huang
Xing-Guo
XG
Tian
D000818:Animals; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D013552:Swine
D012333:RNA, Messenger