Expression of ornithine decarboxylase of Coccidioides immitis in three Escherichia coli strains carrying the lambda DE3 lysogen and an E. coli EWH319 strain odc- null mutant.
Ornithine decarboxylase from respiratory fungal pathogen, Coccidioides immitis, cloned in the pETCiODC plasmid under control of T7lac promoter, was produced in E. coli BL21(DE3), BL21(DE3)pLysS, BLR(DE3) and EWH319 transformant strains. E. coli BL21(DE3)pLysS-pETCiODC expressed the highest specific activity of ODC, suggesting that this strain could be successfully used for protein structure and drug testing studies.
Instituto Tecnológico de Celaya, Departamento de Ingenieráa Bioquímica, Ave. Tecnológico v A. Garcia-Cubas, S/N, Colonia FOVISSSTE, Apartado postal 57, Celaya, Guanajuato, México.
Miguel Angel
MA
Pantoja-Hernández
Claudia Ivonne
CI
Muñoz-Sánchez
Ramón Gerardo
RG
Guevara-González
Enrique
E
Botello-Alvarez
Mario Martín
MM
González-Chavira
Irineo
I
Torres-Pacheco
Lorenzo
L
Guevara-Olvera
D003045:Coccidioides / Q000201:enzymology* / Q000235:genetics*; D004789:Enzyme Activation; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D009955:Ornithine Decarboxylase / Q000096:biosynthesis* / Q000235:genetics*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis; D013045:Species Specificity; D013379:Substrate Specificity; D014169:Transformation, Bacterial
D011994:Recombinant Proteins; D009955:Ornithine Decarboxylase