[Experimental study of the effect of TNP-470 on human salivary adenoid cystic carcinoma cell in vitro].
OBJECTIVE
To investigate the effects of TNP-470 on human salivary adenoid cystic carcinoma cell line ACC M in vitro.


METHODS
Cell proliferation was assessed by MTT assays and dye exclusion counting. Morphological changes of apoptosis were observed with fluorescent microscope. DNA ladder, apoptosis rate and cell cycle were examined by DNA agarose gel electronphores and fluorescence flow cytometry (FCM), respectively.


RESULTS
The 50% inhibitory concentrations (IC50) of TNP-470 on ACC-M cells proliferation by MTT assays and dye exclusion counting were 40.68microg/ml and 46.38microg/ml. Apoptosis were observed by fluorescent microscope. DNA electrophoresis for the cells treated with TNP-470 showed brighter DNA ladder; Sub-G1 peak and G2/M arrest were also determined by FCM (P<0.01).


CONCLUSIONS
TNP-470 has the effect of inducing apoptosis in ACC-M cells in vitro, which may be one of its antitumor mechanisms.
Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China.
Y Q
YQ
Deng
W L
WL
Qiu
R G
RG
He
G C
GC
Lin
W T
WT
Chen
X J
XJ
Zhou