Separation of thiol and cyanide hydrolysis products of chemical warfare agents by capillary electrophoresis.
The fluorescence derivatizing agent, o-phthalaldehyde (OPA), has been applied to the separation and detection of cyanide and several structurally similar thiols by capillary electrophoresis (CE)-laser induced fluorescence (LIF). Of particular interest to this investigation was the separation of 2-dimethylaminoethanethiol, 2-diethylaminoethanethiol, and cyanide, each of which are hydrolysis products or hydrolysis product simulants of the chemical warfare (CW) agents O-ethyl S-2-diisopropylaminoethyl methylphosphonothiolate (VX), O-isobutyl S-2-diethylaminoethyl methylphosphonothiolate (R-VX), and tabun (GA). Other structurally similar thiols simultaneously resolved by this method include 1-pentanethiol and 2-mercaptoethanol. Instrumental parameters were probed and optimum values for capillary length (50 cm) and inner diameter (75 microm), injection time (30 s) and field strength (15 kV) were determined. Sample stacking methods enabled detection limits of 9.3 microg/L for cyanide, 1.8 microg/L for 2-diethylaminoethanethiol, 35 microg/L for 2-dimethylaminoethanethiol, 15 microg/L for 2-mercaptoethanol, and 89 microg/L for 1-pentanethiol. The linearity of the method was verified over an order of magnitude and the reproducibility was found to be 3.0%.
Copper
Christine L
CL
United States Naval Academy, and Chemistry Division, Naval Research Laboratory, 4555 Overlook Avenue SW, Washington, DC 20375-5342, USA.
Collins
Greg E
GE
D002619:Chemical Warfare Agents / Q000737:chemistry* / Q000378:metabolism; D003486:Cyanides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D006868:Hydrolysis; D007536:Isomerism; D007834:Lasers; D015394:Molecular Structure; D013438:Sulfhydryl Compounds / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D013654:Taurine / Q000032:analysis / Q000737:chemistry; D009764:o-Phthalaldehyde / Q000737:chemistry
D002619:Chemical Warfare Agents; D003486:Cyanides; D013438:Sulfhydryl Compounds; D013654:Taurine; D009764:o-Phthalaldehyde