Structure of oligosaccharide side chains of an intestinal immune system modulating arabinogalactan isolated from rhizomes of Atractylodes lancea DC.
An intestinal immune system modulating arabino-3,6-galactan (ALR-5IIa-1-1) has been found in rhizomes of Atractylodes lancea DC. [Planta Medica 1998, 64, 714-719; Carbohydr. Polyms. 2001, 46, 147-156], however other arabino-3,6-galactans from Larix and Acacia failed to express the modulating activity. Degradation of the galactosyl side chains in Araf-side chain-trimmed ALR-5IIa-1-1 (AF-ALR-5IIa-1-1) with an endo-beta-D-(1-->6)-galactanase remarkably decreased the activity of AF-ALR-5IIa-1-1. Structural analysis indicated that the major endo-beta-D-(1-->6)-galactanase-digestable side chains in ALR-5IIa-1-1 are composed of beta-D-(1-->6)-galactopyranosyl oligosaccharides having d.p. 1-8. Because degradation of the beta-D-(1-->3)-galactan backbone in AF-ALR-5IIa-1-1 also significantly reduced its activity, some of these galactosyl side chains attached to beta-D-(1-->3)-galactan backbone are suggested to be responsible for expression of the activity of ALR-5IIa-1-1.
Kitsato Institute for Life Sciences and Graduate school of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo 108-8642, Japan.
Ikue
I
Taguchi
Hiroaki
H
Kiyohara
Tsukasa
T
Matsumoto
Haruki
H
Yamada
D031174:Atractylodes / Q000737:chemistry*; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D005685:Galactans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D007421:Intestine, Small / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D009844:Oligosaccharides / Q000737:chemistry*; D010581:Peyer's Patches / Q000166:cytology / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D027343:Rhizome / Q000737:chemistry*
D016207:Cytokines; D005685:Galactans; D009844:Oligosaccharides; C005653:arabinogalactan