Serotonin increases glial cell line-derived neurotrophic factor release in rat C6 glioblastoma cells.
Antidepressants, which increase monoamine levels, induce glial cell line-derived neurotrophic factor (GDNF) release in C6 cells. Thus, we examined whether monoamines affect on GDNF release in C6 cells. We found that serotonin (5-HT) specifically increased GDNF mRNA expression and GDNF release in a dose- and time-dependent manner. The 5-HT-induced GDNF release was mediated through the MEK/mitogen-activated protein kinase (MAPK) pathway and, at least, 5-HT(2A) receptors. The action of 5-HT on GDNF release may provide important insights into the mechanism of antidepressants.
Department of Psychiatry and Neuroscience, Institute of Clinical Research, National Kure Medical Center, 3-1 Aoyama, Kure 737-0023, Japan. hisaoka@kure-nh.go.jp
Kazue
K
Hisaoka
Akira
A
Nishida
Minoru
M
Takebayashi
Tetsuzo
T
Koda
Shigeto
S
Yamawaki
Yoshihiro
Y
Nakata
D000818:Animals; D045744:Cell Line, Tumor; D051100:Glial Cell Line-Derived Neurotrophic Factor; D005909:Glioblastoma / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000096:biosynthesis / Q000378:metabolism*; D051381:Rats; D012701:Serotonin / Q000494:pharmacology*
C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D012701:Serotonin