Studies on structural and immunogenic polypeptides of hydropericardium syndrome virus by SDS-PAGE and western blotting.
The polypeptide pattern of a local isolate of a virus causing hydropericardium syndrome was analyzed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. A total of 12 polypeptides ranging in molecular weight between 13.8 and 110.0 kDa were observed. Western blot analysis of structural polypeptides revealed seven immunogenic polypeptides ranging in molecular weight between 15.8 and 110.0 kDa.
Kumar
Rajesh
R
Department of Veterinary Microbiology, College of Veterinary Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar 263 145, U.S. Nagar, Uttaranchal, India. rajeshvet@rediffmail.com
Chandra
Rajesh
R
D000256:Adenoviridae / Q000276:immunology / Q000378:metabolism*; D000257:Adenoviridae Infections / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D002460:Cell Line; D002642:Chick Embryo; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D008970:Molecular Weight; D010496:Pericardium / Q000473:pathology / Q000821:virology; D011201:Poultry Diseases / Q000821:virology*; D014764:Viral Proteins / Q000276:immunology / Q000378:metabolism*
D014764:Viral Proteins