[Neutrophil activation by sea hydrobiont biopolymers].
Biopolymers of sea hydrobionts such as mytilan, alpha-1,4;1,6-D-glycan isolated from the muntle of the mussel Crenomytilus grayanus; translam, beta-1,3;1,6-D-glucan isolated from the seaweed Laminaria cichorioides; fucoidan, a sulfated polysccharide isolated from the algae Fucus evanescens; zosterin, a pectin isolated from sea grass of the family Zosteraceae were comparatively studied. The mechanisms of the phagocyte activation were investigated and the dose-dependent ability of the biopolymers to increase in vitro adhesion of the intact cells and to restore the neutrophil functions at cyclophosphamide-induced immunodepression was detected. The neutrophil activation by mytilan, zosterin and fucoidan linked with the adhesion potentiation was shown to be associated with their ability to increase the number of the adhesion receptors and in particular CD116b on the cell surface. The lower potential of the neutrophils preincubated in vitro with high doses of translam beta-glucan could be due to blockade of the beta-glucan receptors participating in the complex multicomponent adhesion process. The use of the biopolymers of the sea hydrobionts of the glycobiological nature for modulation of the immunity processes provided rather convenient in vivo management of intracellular processes through direct and competing carbohydrate specific interactions of the modifiers with the membrane receptors and formation of active and inactive lectin-glycoligand and carbohydrate-carbohydrate complexes.
Research Institute of Epidemiology and Microbiology, Siberian Branch, Russian Academy of Medical Sciences, Vladivostok.
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Zaporozhets
D000276:Adjuvants, Immunologic / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D049872:Bivalvia; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D005936:Glucans / Q000494:pharmacology; D008297:Male; D008386:Marine Biology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D023421:Models, Animal; D018375:Neutrophil Activation; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D000459:Phaeophyceae; D010587:Phagocytosis; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis / Q000096:biosynthesis; D011971:Receptors, Immunologic / Q000032:analysis / Q000037:antagonists & inhibitors; D035962:Zosteraceae
D000276:Adjuvants, Immunologic; D005936:Glucans; D011134:Polysaccharides; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; D011971:Receptors, Immunologic; C045268:beta-glucan receptor; C033671:epiglucan; C070927:zosterin; C030207:mytilan; C007789:fucoidan