Efficient application of monolithic silica column to determination of illicit heroin street sample by HPLC.
In this paper, an HPLC method is proposed for a routine, rapid and simple analysis of heroin samples confiscated from the illicit market, based on a new type of packing for HPLC columns (monolithic silica). Acetonitrile and pH 3.5 phosphate buffer solution were used under both isocratic and gradient conditions. Under our analytical conditions, all the components of a typical mixture of an illicit heroin sample proved to be fully separated into well-resolved peaks in 7 min. Analytical linearity and accuracy of the method were also studied for all analytes using tetracaine hydrochloride as the internal standard.
Macchia
Marco
M
Department of Pharmaceutical Sciences, University of Pisa, via Bonanno 6, Pisa 56126, Italy. mmacchia@farm.unipi.it
Bertini
Simone
S
Mori
Claudio
C
Orlando
Caterina
C
Papi
Chiara
C
Placanica
Giorgio
G
D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D003932:Heroin / Q000032:analysis*; D013287:Illicit Drugs / Q000032:analysis*; D009294:Narcotics / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012822:Silicon Dioxide; D012996:Solutions
D013287:Illicit Drugs; D009294:Narcotics; D012996:Solutions; D003932:Heroin; D012822:Silicon Dioxide