Study on interleukin-18 gene transfer into human breast cancer cells to prevent tumorigenicity.
To study the effect of interleukin-18 gene transfection on the tumorigenesis of breast cancer cell line Bacp37, human breast cancer cell line Bcap37 were transfected with Lipofectamine and selected by G418. The biological expression of rhIL-18 was tested by RT-PCR and ELISA method; nude mice were injected with Bcap37 cell with or without the hIL-18 gene. The hIL-18 cDNA was successfully integrated into Bcap37 cell; 126.3+/-4.5 pg hIL-18 secreted by one million transduced cells in 24 hours. Nude mice injected with IL-18 gene engineered Bcap37 cell had no tumor growth. These findings indicated that human breast cancer cells were successfully modified by the gene of IL-18 cytokine; the IL-18 gene engineered Bcap37 cells secreted hIL-18 and lost their tumorigenicity. The Bcap37 cells transduced with IL-18 gene may be used as breast cancer vaccine.
Cancer Institute, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.
Ming-yong
MY
Han
Shu
S
Zheng
Jin-ming
JM
Yu
Jia-ping
JP
Peng
Qi-sen
QS
Guo
Jia-lin
JL
Wang
D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics / Q000276:immunology / Q000628:therapy*; D019496:Cancer Vaccines / Q000235:genetics / Q000276:immunology; D045744:Cell Line, Tumor; D005260:Female; D015870:Gene Expression; D018014:Gene Transfer Techniques; D006801:Humans; D020382:Interleukin-18 / Q000235:genetics*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D000091402:Prohibitins; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D014162:Transfection; D014183:Transplantation, Heterologous
D019496:Cancer Vaccines; D020382:Interleukin-18; C000717331:PHB2 protein, human; C000717333:Phb2 protein, mouse; D000091402:Prohibitins; D012333:RNA, Messenger; D011994:Recombinant Proteins