RACK1 is a functional target of the E1A oncoprotein.
The adenoviral E1A proteins have been implicated in promotion of proliferation and transformation, inhibition of differentiation, induction of apoptosis, regulation of transcription, and suppression of tumor growth. The ability of E1A to override the fundamental controls of host cells is based on its ability to physically interact with several cellular proteins. We recently characterized RACK1 as a new E1A-interacting protein. In this report, we show that the extreme N-terminal region of E1A, spanning from aminoacids 1-36, and the conserved WD regions of RACK1 are responsible for this interaction. We also demonstrate that E1A and RACK1 colocalize at the perinuclear membrane in the cells. Furthermore, we provide evidence that E1A is able to antagonize the inhibitory effects of RACK1 on Src activity. These results suggest that RACK1 signaling pathway may be a functional target of E1A, contributing to E1A oncogenic effect in the host cells.
Severino
Anna
A
Laboratory C, Department for the Development of Therapeutic Programs, Center for Experimental Research, Regina Elena Cancer Institute, Rome, Italy.
Baldi
Alfonso
A
Cottone
Giuliano
G
Han
Mei
M
Sang
Nianli
N
Giordano
Antonio
A
Mileo
Anna Maria
AM
Paggi
Marco G
MG
De Luca
Antonio
A
D017500:Adenovirus E1A Proteins / Q000502:physiology*; D000818:Animals; D001665:Binding Sites; D045744:Cell Line, Tumor; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D041681:NIH 3T3 Cells; D011233:Precipitin Tests; D011485:Protein Binding; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface / Q000502:physiology*; D014162:Transfection; D019061:src-Family Kinases / Q000378:metabolism
D017500:Adenovirus E1A Proteins; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface; D019061:src-Family Kinases