[Investigation on the distribution and their effects on reproduction traits of three major genes in Jinhua pigs].
The PCR-RFLP technique was applied to analyze the distribution of estrogen receptor (ESR) gene, follicle-stimulating hormone beta subunit(FSH beta) gene and prolactin receptor (PRLR) gene in three lines of Jinhua pigs. The effect of ESR, FSH beta and PRLR on the reproductive traits was analyzed by the SPSS software. The results showed that: The genotype frequencies of AA, AB, BB for ESR were 0.0237, 0.3609, 0.6154 for Jinhua pig line I, 0, 0.5333, 0.4667 for Jinhua pig line II and 0.2909, 0.6, 0.1091 for Jinhua pig line III, respectively; The genotype frequencies of AA, AB, BB for PRLR were 0.2560, 0.3929, 0.3512 for Jinhua pig line I, 0.2, 0.2667, 0.5333 for Jinhua pig line II and 0.1273, 0.4909, 0.3818 for Jinhua pig line III, respectively; But only the Jinhua pig line I had the polymorphism of FSH beta, and with the low frequency of favorable allele B (0.0120). Significant associations were found for the ESR locus with the number of born alive (NBA) in later parities, the NBA and the total number of born (TNB) of all parities(P < 0.05) and the NBA of later parities (P = 0.061) in Jinhua pig line I. The additive effects of allele B were 0.70 and 0.66 for the NBA in later parities and all parities respectively, and 0.90 and 0.84 for the TNB in later parities and all parities respectively. No significant associations were found for the PRLR locus with the reproductive traits for Jinhua pigs in all parities and significant interactions of ESR and PRLR were found for the TNB and the NBA in all parities (P < 0.05) and for the female birth weight (FBW) in later parities (P < 0.01).
College of Animal Science, Zhejiang University, Hangzhou 310029, China. ningying@hzcnc.com
Ning-Ying
NY
Xu
Sheng-Qiao
SQ
Zhang
Shu-Hong
SH
Peng
D000818:Animals; D001724:Birth Weight; D001835:Body Weight; D037201:Follicle Stimulating Hormone, beta Subunit / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D011960:Receptors, Estrogen / Q000235:genetics*; D011981:Receptors, Prolactin / Q000235:genetics*; D012098:Reproduction; D013552:Swine / Q000235:genetics* / Q000502:physiology
D037201:Follicle Stimulating Hormone, beta Subunit; D011960:Receptors, Estrogen; D011981:Receptors, Prolactin