[Experimental research of PDE5 activity in corpora cavernosa of ganyu qizhi rat model].
OBJECTIVE
To study the PDE5 activity in corpora cavernosa of the Ganyu Qizhi model penis and the effect of the Chinese herbal medicine Shugan Liqi Huoxue (SLH) ointment on it.


METHODS
Non-injury stress stimulus method similar to human spirit stress was used to extablish the Ganyu Qizhi animal(rat) model, and the PDE5 activity in corpora cavernosa of the rat penis was measured by the method of immunohistochemistry and computer image analysis.


RESULTS
The PDE5 activity in corpora cavernosa of the high-dosage SLH group was significantly different from that of the model group (P < 0.01).


CONCLUSIONS
Ganyu Qizhi may increase the PDE5 activity in corpora cavernosa of the penis while SLH can reduce such activity.
Chang
Degui
D
Department of Andrology, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 610072, China. changdg@mail.sc.cnicfo.net
Wang
Zhengyi
Z
Zhu
Bide
B
Wang
Jiuyuan
J
Zhang
Shuwu
S
Hu
Zhiping
Z
D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000378:metabolism*; D000818:Animals; D054706:Cyclic Nucleotide Phosphodiesterases, Type 5; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007172:Erectile Dysfunction / Q000209:etiology; D008297:Male; D008516:Medicine, Chinese Traditional; D023421:Models, Animal; D010413:Penis / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D054706:Cyclic Nucleotide Phosphodiesterases, Type 5; C517381:PDE5A protein, human; C517384:Pde5a protein, rat