[Study on a sensitive setup of capillary electrophoresis with laser induced fluorescence detector and its application].
OBJECTIVE
To develop a setup of capillary electrophoresis with laser induced fluorescence detector for separating and detecting DNA fragments and protein.


METHODS
The setup is assembled of He-Ne Laser with 543.5 nm as the excitation light, filter, monochromator, photomultiplier tube and a computer for controlling the system and collecting the electropherograms.


RESULTS
The design of the setup and the analytical parameters were optimized. The electropherograms with high Signal-to-Noise were obtained by the laser induced fluorescence detector-capillary electrophoretic setup. For Sulforhdamine B, the detection limit was 10(-9) mol/L, and the linear range from 1 x 10(-6) mol/L to 1 x 10(-9) mol/L. The PCR products, the digested products by Hae III and BstE of mycobacterium DNA, and the human serum albumin were determined by the successful application of this setup.


CONCLUSIONS
The setup is characterized by simple structure, low cost and high sensitivity. Compared with traditional agarose gel electrophoresis, capillary electrophoresis with laser induced fluorescence detector is more remarkable in resolution and detection time. It can be applied as a more effective means for analysis of DNA fragments and protein.
Li
Yuan-qian
YQ
Department of Sanitary Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Yang
Jing-guo
JG
Zhou
Ying
Y
Zou
Xiao-li
XL
Mi
Jian-ping
JP
Zeng
Hong-yan
HY
D004247:DNA / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D005453:Fluorescence; D007834:Lasers; D011506:Proteins / Q000032:analysis; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation
D011506:Proteins; D004247:DNA