Transcriptional analysis of the ostA/imp gene involved in organic solvent sensitivity in Escherichia coli.
Transcriptional initiation sites of the ostA gene involved in organic solvent sensitivity in Escherichia coli were found by primer extension analysis. Two transcriptional initiation sites were newly identified at -133 and -48 nucleotides from the initiation codon of ostA, but the previously reported sigmaE-dependent one at -227 could not be detected. No heat-inducible expression of ostA was observed by Northern blotting analysis, indicating that the contribution of sigmaE-dependent transcription was very small if any. SigmaD-dependent promoter-like sequences were found just upstream of the newly identified transcriptional initiation sites by computer-aided analysis. Deletion analysis of ostA-lacZ fusions demonstrated that these two promoters contributed almost equally to the constitutive expression of the ostA gene.
Ohtsu
Iwao
I
Department of Biological Information, Tokyo Institute of Technology, Yokohama, Japan.
Kakuda
Nobuto
N
Tsukagoshi
Norihiko
N
Dokyu
Noriyuki
N
Takagi
Hiroshi
H
Wachi
Masaaki
M
Aono
Rikizo
R
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D015152:Blotting, Northern; D002874:Chromosome Mapping; D017931:DNA Primers; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D007763:Lac Operon / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D012329:RNA, Bacterial / Q000032:analysis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012997:Solvents / Q000494:pharmacology*; D015533:Transcriptional Activation / Q000235:genetics*
D001425:Bacterial Outer Membrane Proteins; D017931:DNA Primers; D029968:Escherichia coli Proteins; C475958:LptD protein, E coli; D012329:RNA, Bacterial; D011993:Recombinant Fusion Proteins; D012997:Solvents