Elimination of chrysanthemum stunt viroid from an infected chrysanthemum cultivar by shoot regeneration from a leaf primordium-free shoot apical meristem dome attached to a root tip.
In this research we eliminated chrysanthemum stunt viroid (CSVd) from a highly infected chrysanthemum cultivar using a newly established method. 'Piato' is one of the most difficult cultivars in which to obtain CSVd-free plants by conventional methods. Leaf primordium-free shoot apical meristems (LP-free SAMs) of 'Piato' plants were dissected and attached to CSVd-free chrysanthemum or cabbage root tips. As shown by nested-PCR, CSVd was not detected in some shoots regenerated on both types of root tip. The production rates of CSVd-free plants using chrysanthemum and cabbage root tips were 14% and 3%, respectively. Regeneration of plants from LP-free SAMs of chrysanthemum plants by attaching these SAMs to root tips is an efficient method of generating CSVd-free chrysanthemum plants.
Laboratory of Vegetable and Ornamental Horticulture, Department of Agronomy and Horticultural Science, Graduate School of Agriculture, Kyoto University, Sakyo-ku, 606-8502 Kyoto, Japan. mune@kais.kyoto-u.ac.jp
M
M
Hosokawa
A
A
Otake
K
K
Ohishi
E
E
Ueda
T
T
Hayashi
S
S
Yazawa
D001937:Brassica / Q000235:genetics; D031202:Chrysanthemum / Q000254:growth & development / Q000821:virology*; D003470:Culture Media / Q000494:pharmacology; D018519:Meristem / Q000254:growth & development / Q000821:virology; D018520:Plant Shoots / Q000254:growth & development* / Q000821:virology; D010942:Plant Viruses / Q000302:isolation & purification; D012038:Regeneration; D014772:Viroids / Q000302:isolation & purification*
D003470:Culture Media