Crystallization and preliminary X-ray diffraction study of fructan 1-exohydrolase IIa from Cichorium intybus.
Fructan 1-exohydrolase IIa (1-FEH IIa), a plant enzyme involved in fructan breakdown, has been crystallized using the hanging-drop vapour-diffusion method at 277 K. The crystals are tetragonal, belonging to space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = 139.83, b = 139.83, c = 181.94 A. Calculation of the Matthews coefficient indicates there to be two or three molecules in the asymmetric unit. Synchrotron radiation was used to collect a complete native data set to a resolution of 2.35 A.
Laboratorium Voor Analytische Chemie en Medicinale Fysicochemie, Faculteit Farmaceutische Wetenschappen, K.U. Leuven, E. Van Evenstraat 4, B-3000 Leuven, Belgium.
Maureen
M
Verhaest
Wim
W
Van den Ende
Midori
M
Yoshida
Katrien
K
Le Roy
Yves
Y
Peeraer
Stefaan
S
Sansen
Camiel J
CJ
De Ranter
André
A
Van Laere
Anja
A
Rabijns
D018651:Cichorium intybus / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D005630:Fructans / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism
D005630:Fructans; D010940:Plant Proteins; D006026:Glycoside Hydrolases; C044118:fructan beta-fructosidase