Antiviral activity of caspase inhibitors: effect on picornaviral 2A proteinase.
Peptide-based fluoromethyl ketones have been considered for many years to be highly specific caspase inhibitors distinctly blocking the progress of apoptosis in a variety of systems. Here we demonstrate that these compounds can significantly reduce rhinovirus multiplication in cell culture. In their methylated forms they block eIF4GI cleavage in vivo and in vitro and inhibit the activity of picornaviral 2A proteinases.
Department of Medical Biochemistry, Division of Biochemistry, University of Vienna, Dr. Bohr Gasse 9/3, A-1030 Vienna, Austria.
Luiza
L
Deszcz
Joachim
J
Seipelt
Elena
E
Vassilieva
Andreas
A
Roetzer
Ernst
E
Kuechler
D000998:Antiviral Agents / Q000378:metabolism* / Q000627:therapeutic use; D017209:Apoptosis; D061945:Caspase Inhibitors; D010450:Endopeptidases / Q000378:metabolism*; D039603:Eukaryotic Initiation Factor-4G; D006367:HeLa Cells; D006801:Humans; D010446:Peptide Fragments / Q000378:metabolism; D010448:Peptide Initiation Factors / Q000378:metabolism; D010850:Picornaviridae Infections / Q000188:drug therapy*; D011499:Protein Processing, Post-Translational; D012367:RNA, Viral / Q000378:metabolism; D012229:Rhinovirus / Q000201:enzymology* / Q000235:genetics; D013997:Time Factors
D000998:Antiviral Agents; D061945:Caspase Inhibitors; C113920:EIF4G1 protein, human; D039603:Eukaryotic Initiation Factor-4G; D010446:Peptide Fragments; D010448:Peptide Initiation Factors; D012367:RNA, Viral; D010450:Endopeptidases