Monomer surface modifications for rapid peptide analysis by capillary electrophoresis and capillary electrochromatography coupled to electrospray ionization-mass spectrometry.
In this study, positively charged alkylaminosilyl monomers were used to modify the inner surface of fused silica capillaries, which subsequently were employed in capillary electrophoresis (CE) and capillary electrochromatography (CEC). The obtained surfaces yield a reversed electroosmotic flow (EOF) and have varying carbon chain lengths, that interact with the analytes and give chromatographic retention. The coating procedure is very simple and fast. The performance of the modified capillaries was evaluated regarding pH influence on EOF and chromatographic interactions. The experiments were conducted with UV and mass spectrometry (MS) and applied to the separation of various neuropeptides. The derivatized surfaces showed a linear (R(2) approximately 0.99) pH dependence with isoelectric points (pI) at 8.6-8.8. Rapid separations of peptide standards and a protein digest with efficiencies as high as 5 x 10(5) plates/m were performed.
Department of Analytical Chemistry, Uppsala University Box 599, SE-75124 Uppsala, Sweden.
Nina
N
Johannesson
Magnus
M
Wetterhall
Karin E
KE
Markides
Jonas
J
Bergquist
D002845:Chromatography / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006361:Heating; D006863:Hydrogen-Ion Concentration; D010455:Peptides / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012821:Silanes / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D014357:Trypsin / Q000737:chemistry; D014466:Ultraviolet Rays
D010455:Peptides; D012821:Silanes; D012710:Serum Albumin, Bovine; D014357:Trypsin