Two new tumor-specific antigenic peptides encoded by gene MAGE-C2 and presented to cytolytic T lymphocytes by HLA-A2.
We have identified 2 antigens recognized by several melanoma-specific cytolytic T lymphocyte clones isolated from a melanoma patient with a clinical history of tumor regression after immunotherapy. Both antigens are presented by HLA-A2 and encoded by gene MAGE-C2, a cancer-germline gene shown previously to be silent in normal somatic tissues and expressed in 40% of melanomas and in other tumor types. One antigen corresponds to peptide ALKDVEERV(336-344), whereas the other corresponds to peptide LLFGLALIEV(191-200). The CTL clones recognizing these 2 peptides also recognized allogeneic tumor cell lines expressing MAGE-C2 and HLA-A2. These 2 new peptides are the first known MAGE-C antigens and represent promising targets for cancer immunotherapy.
Ma
Wenbin
W
Ludwig Institute for Cancer Research, Brussels Branch, Avenue Hippocrate 74, UCL 7459, B-1200 Brussels, Belgium.
Germeau
Catherine
C
Vigneron
Nathalie
N
Maernoudt
Anne-Sophie
AS
Morel
Sandra
S
Boon
Thierry
T
Coulie
Pierre G
PG
Van den Eynde
Benoît J
BJ
D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000235:genetics*; D018076:DNA, Complementary / Q000378:metabolism; D004273:DNA, Neoplasm / Q000378:metabolism; D015789:HLA-A2 Antigen / Q000276:immunology*; D006801:Humans; D008207:Lymphatic Metastasis; D008545:Melanoma / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000951:Antigens, Neoplasm; D018076:DNA, Complementary; D004273:DNA, Neoplasm; D015789:HLA-A2 Antigen; C405429:MAGEC2 protein, human; D009363:Neoplasm Proteins