In vitro and in vivo immunotoxic and immunomodulatory effects of nonsupplemented and selenium-supplemented cigarette smoke condensate.
Cigarette smoke condensate has been evaluated for its in vitro and in vivo immunotoxic and immunomodulatory properties. It was found that cigarette smoke condensate used in vitro at concentration from 6.6 to 20 microg/ml exerted pronounced inhibitory effects upon cell surface antigen-presenting major histocompatibility complex class I (MHC-I) expression and immunoglobulin (Ig) synthesis. In vivo, i.p. administration of cigarette smoke condensate to C57BL/6 mice before challenging with ovalbumin (OVA) antigen, has led to a decrease of anti-OVA specific antibody response. This inhibition affected more Ig protein synthesis than membrane bound MHC-I expression. Supplementation with selenium (Se) significantly reduced the inhibitory effects both in vitro and in vivo.
Laboratoire de Toxicologie, Faculté des Sciences Pharmaceutiques et Biologiques, Université René Descartes, Paris, France.
Pascale
P
Nguyen Van Binh
Dong
D
Zhou
Françoise
F
Baudouin
Chantal
C
Martin
Martine
M
Radionoff
Hélène
H
Dutertre
Véronique
V
Marchand
Marc
M
Thevenin
Jean-Michel
JM
Warnet
Huynh Thien
HT
Duc
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000633:toxicity; D045744:Cell Line, Tumor; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis / Q000097:blood; D007136:Immunoglobulins / Q000096:biosynthesis / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology; D012643:Selenium / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D012906:Smoke / Q000009:adverse effects; D014026:Nicotiana
D000276:Adjuvants, Immunologic; D000975:Antioxidants; D015395:Histocompatibility Antigens Class I; D007136:Immunoglobulins; D012906:Smoke; D010047:Ovalbumin; D012643:Selenium