Display of heterologous antigens on the Bacillus subtilis spore coat using CotC as a fusion partner.
We report the use of CotC, a major component of the Bacillus subtilis spore coat, as a fusion partner for the expression of two heterologous antigens on the spore coat. Recombinant spores expressing tetanus toxin fragment C (TTFC) of Clostridium tetani or the B subunit of the heat-labile toxin of Escherichia coli (LTB) were used for oral dosing and shown to generate specific systemic and mucosal immune responses in a murine model. This report, expanding the previously described expression of TTFC on the spore surface by fusion to CotB [J Bacteriol 183 (2001) 6294] and its use for oral vaccination [Infect Immun 71 (2003) 2810] shows that different antigens can be successfully presented on the spore coat and supports the use of the spore as an efficient vehicle for mucosal immunisation.
Mauriello
Emilia M F
EM
Dipartimento di Fisiologia Generale ed Ambientale, Sezione di Microbiologia, Università Federico II, Napoli, Italy.
Duc
Le H
le H
Isticato
Rachele
R
Cangiano
Giuseppina
G
Hong
Huynh A
HA
De Felice
Maurilio
M
Ricca
Ezio
E
Cutting
Simon M
SM
D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D001412:Bacillus subtilis / Q000737:chemistry / Q000276:immunology*; D001427:Bacterial Toxins / Q000276:immunology; D015153:Blotting, Western; D002876:Chromosomes, Bacterial / Q000276:immunology; D003512:Cyclohexanones / Q000737:chemistry / Q000276:immunology*; D004768:Enterotoxins / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D029968:Escherichia coli Proteins; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics; D018928:Immunity, Mucosal / Q000276:immunology; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology; D013171:Spores, Bacterial / Q000737:chemistry / Q000276:immunology*; D013742:Tetanus / Q000276:immunology; D013744:Tetanus Toxin / Q000276:immunology; D014169:Transformation, Bacterial; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D003512:Cyclohexanones; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D013744:Tetanus Toxin; D014614:Vaccines, Synthetic; C040635:tetanus toxin fragment C; C011202:2-crotonyloxymethyl-(4R,5R,6R)-4,5,6-trihydroxycyclohex- 2-enone; C027538:heat-labile enterotoxin, E coli