Proteomic analysis of selected prognostic factors of breast cancer.
The incidence of breast cancer is on the rise but as yet there is no guaranteed beneficial treatment for many of the sufferers. The treatments specific for breast and other hormone-sensitive cancers work well at times, however, the population of women that they will benefit is relatively small. Many are limited to surgical, chemotherapy, and radiotherapy options. Here, using two-dimensional electrophoresis (2-DE) in conjunction with a silver stain and Western blotting approach, we attempt to locate selected known prognostic markers for breast cancer. With these results, we can exclude these proteins from the future search for potential pharmaceutical targets, using the same techniques. The proteins that were located include the estrogen receptor-alpha, beta-casein, cytokeratin 7, calponin and bax. For each protein an estimated M(r) and pI was gained. Each protein was found in multiple variants. By locating these proteins the number of unknown proteins found on the 2-DE gel has been reduced, helping the future search for novel markers that are shown as being differentially expressed between healthy and cancerous tissue samples.
Department of Surgery, University of Tasmania, Hobart, Australia.
Kylie
K
Roberts
Kiron
K
Bhatia
Peter
P
Stanton
Roger
R
Lord
D015153:Blotting, Western; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002364:Caseins / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D047628:Estrogen Receptor alpha; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007091:Image Processing, Computer-Assisted; D015151:Immunoblotting; D053552:Keratin-7; D007633:Keratins / Q000378:metabolism; D008840:Microfilament Proteins; D011379:Prognosis; D011506:Proteins / Q000378:metabolism; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2; D011960:Receptors, Estrogen / Q000378:metabolism; D050717:Retinoblastoma-Like Protein p130; D016622:Silver Staining; D051028:bcl-2-Associated X Protein; D000096985:Calponins
D002135:Calcium-Binding Proteins; D002364:Caseins; D047628:Estrogen Receptor alpha; D053552:Keratin-7; D007633:Keratins; D008840:Microfilament Proteins; D011506:Proteins; D020543:Proteome; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D011960:Receptors, Estrogen; D050717:Retinoblastoma-Like Protein p130; D051028:bcl-2-Associated X Protein; D000096985:Calponins; C494826:BAX protein, human; C507497:KRT7 protein, human; C493837:RBL2 protein, human