Long-term preservation of fungal isolates in commercially prepared cryogenic microbank vials.
Since 1994, 6,198 yeasts and 391 molds belonging to 25 and 37 species, respectively, were stored in Microbank cryogenic vials at >/=-130 degrees C in liquid nitrogen and at -70 degrees C in a freezer. All of the isolates, with the exception of 45 yeasts and 15 dermatophytes, were recovered from both storage temperatures. Good reproducibility was demonstrated for amphotericin B, fluconazole, and voriconazole MICs determined for random isolates.
Division of Infectious Diseases, Medical Mycology Research Laboratory, VCU Medical Center, Richmond, Virginia 23298-0049, USA. avingrof@hsc.vcu.edu
A
A
Espinel-Ingroff
D
D
Montero
E
E
Martin-Mazuelos
D003883:Arthrodermataceae / Q000302:isolation & purification; D015925:Cryopreservation; D005658:Fungi / Q000302:isolation & purification*; D003904:Mitosporic Fungi / Q000302:isolation & purification; D009172:Mycology / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D015003:Yeasts / Q000302:isolation & purification
D011933:Reagent Kits, Diagnostic