[Effects of high concentrations insulin on VEGF expression in cultured Müller cells].
OBJECTIVE
To investigate the effects of high concentrations of insulin on the expression of vascular endothelial growth factor (VEGF) in cultured rabbit retinal Müller cells.


METHODS
Immunocytochemistry, in situ hybridization and the ELISA method were used to study the effects of different concentrations of insulin on the expression of vascular endothelial growth factor (VEGF) in cultured rabbit retinal Müller cells qualitatively and quantitatively.


RESULTS
VEGF expression was enhanced obviously by the insulin, which regulated the expression of VEGF at the transcription level.


CONCLUSIONS
VEGF expression in cultured Müller cells can be enhanced by high concentrations of insulin. Müller cells play a potential role in the development of neovascularization of diabetic retinopathy.
Song
E
E
Department of Ophthalmology, the First Hospital, Jilin University, Changchun 130021, China.
Wang
Yue-hui
YH
Xu
Qi
Q
Wang
Xin-rui
XR
Zhu
Xiang-hong
XH
Wu
Jia-xiang
JX
D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D011817:Rabbits; D012160:Retina / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis* / Q000235:genetics
D007328:Insulin; D042461:Vascular Endothelial Growth Factor A