Determination of the genus-specific antigens in outer membrane proteins from the strains of Leptospira interrogans and Leptospira biflexa with different virulence.
OBJECTIVE
To determine the existence of genus-specific antigens in outer membrane proteins (OMPs) of leptospira with different virulence.


METHODS
Microscope agglutination test (MAT) was applied to detect the agglutination between commercial rabbit antiserum against leptospiral genus-specific TR/Patoc I antigen and 17 strains of Leptospira interrongans belonging to 15 serogroups and 2 strains of Leptospira biflexa belonging to 2 serogroups. The outer envelopes (OEs) of L.interrogans serogroup Icterohaemorrhagiae serovar lai strain lai (56601) with strong virulence and serogroup Pomona serovar pomona strain Luo (56608) with low virulence, and L.biflexa serogroup Semaranga serovar patoc strain Patoc I without virulence were prepared by using the method reported in Auran et al.(1972). OMPs in the OEs were obtained by treatment with sodium deoxycholate. SDS-PAGE and western blot were used for analyzing the features of the OMPs on electrophoretic pattern and the immunoreactivity to the antiserum against TR/Patoc I antigen, respectively.


RESULTS
All the tested strains belonging to different leptospiral serogroups agglutinated to the antiserum against leptospiral genus-specific TR/Patoc I antigen with agglutination titers ranging from 1:256-1:512. A similar SDS-PAGE pattern of the OMPs from the three strains of leptospira with different virulence was shown and the molecular weight of a major protein fragment in the OMPs was found to be approximately 60 KDa. A positive protein fragment with approximately 32 KDa confirmed by Western blot, was able to react with the antiserum against leptospiral genus-specific TR/Patoc I antigen, and was found in each the OMPs of the three stains of leptospira.


CONCLUSIONS
There are genus-specific antigens on the surface of L.interrogans and L.biflexa. The OMP with molecular weight of 32 KDa may be one of the genus-specific protein antigens of leptospira.
Luo
Yi-hui
YH
Department of Medical Microbiology and Parasitology, College of Medical Science, Zhejiang University, Hangzhou 310006, China.
Yan
Jie
J
Mao
Ya-fei
YF
Li
Shu-ping
SP
D000372:Agglutination Tests; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000145:classification* / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D007919:Leptospira / Q000145:classification / Q000276:immunology* / Q000472:pathogenicity; D007921:Leptospira interrogans / Q000145:classification / Q000276:immunology* / Q000472:pathogenicity; D008970:Molecular Weight; D011817:Rabbits; D013045:Species Specificity; D014774:Virulence
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins