Human papillomavirus type 16 E6 and E7 genotypes in head-and-neck carcinomas.
Human papillomavirus type 16 (HPV16) is associated with squamous cell carcinomas of the head and neck (HNSCC) particularly from the Waldeyer's tonsillar ring. A causal role of HPV16 in carcinogenesis is linked to the activity of the viral oncoproteins E6 and E7 which inactivate the cellular tumor suppressors p53 and pRB, respectively. Lack of E6 expression in HPV16-positive HNSCC has been reported, in some cases caused by disruption of the E6 gene. We have examined the status of the HPV16 E6-E7 gene region in tumor and metastasis samples of 24 HNSCC patients employing genomic PCR. No cases with a disrupted E6-E7 region could be identified. Sequence analysis of the E6-E7 segments revealed three different HPV16 E6-E7 genotypes: the HPV16 prototype (6 of 21 cases), the E6 variant T350G (8 of 21 cases), and the E6-E7 variant A131G/C712A (7 of 21 cases). The E6 variants T350G and A131G have been associated with increased oncogenic potential in cervical cancer patients depending on host genetic factors. Their high prevalence in the HNSCC samples studied indicates that they may be important also in HNSCC development.
Department of Otorhinolaryngology, Christian-Albrechts-University, Arnold-Heller-Str. 14, D-24105 Kiel, Germany.
Markus
M
Hoffmann
Claudia
C
Lohrey
Andreas
A
Hunziker
Tomas
T
Kahn
Elisabeth
E
Schwarz
D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000821:virology*; D004273:DNA, Neoplasm / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000821:virology*; D006801:Humans; D008207:Lymphatic Metastasis; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics*; D050725:Papillomavirus E7 Proteins; D016133:Polymerase Chain Reaction / Q000379:methods; D012097:Repressor Proteins
D004273:DNA, Neoplasm; D004279:DNA, Viral; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16