Synthesis of 7'-[123I]iodo-D-luciferin for in vivo studies of firefly luciferase gene expression.
D-(-)-2-(6'-hydroxy-7'-[(123)I]iodobenzothiazolyl)-delta(2)-thiazoline-4-caroxylic acid (7'-[(123)I]iodo-D-luciferin) was synthesized as a novel reporter probe for in vivo studies of firefly luciferase gene expression. 7'-Iodo-D-luciferin, a nonradioactive standard, was synthesized and showed the binding property (K(M)=4.28 microM) similar to that of D-luciferin (2.53 microM) for firefly luciferase in luminescence assay.
Department of Nuclear Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Ilwon-dong, Kangnam-ku, Seoul 135-710, South Korea.
Sang-Yoon
SY
Lee
Yearn Seong
YS
Choe
Kyung-Han
KH
Lee
Jeewoo
J
Lee
Yong
Y
Choi
Byung-Tae
BT
Kim
D000818:Animals; D001517:Coleoptera; D049454:Firefly Luciferin / Q000138:chemical synthesis* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D007457:Iodine Radioisotopes / Q000737:chemistry*; D008156:Luciferases / Q000096:biosynthesis* / Q000235:genetics
D007457:Iodine Radioisotopes; D049454:Firefly Luciferin; D008156:Luciferases