Direct monitoring of enzymatic glucan hydrolysis on a 27-MHz quartz-crystal microbalance.
By using a highly sensitive 27-MHz glucan-immobilized quartz-crystal microbalance, we could follow kinetically all processes (enzyme binding and release, kon and koff, and intramolecular hydrolysis rates, kcat) of glucan hydrolysis by glucoamylase by detecting directly the formation and decay of the enzyme-substrate complex as mass changes.
Department of Biomolecular Engineering, Tokyo Institute of Technology and CREST, JST, Nagatsuda, Midori-ku, Yokohama 226-8501, Japan.
Hidekazu
H
Nishino
Takanori
T
Nihira
Toshiaki
T
Mori
Yoshio
Y
Okahata
D000687:Amylopectin / Q000737:chemistry* / Q000378:metabolism; D001234:Aspergillus niger / Q000201:enzymology; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry* / Q000378:metabolism; D005936:Glucans / Q000737:chemistry* / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D008832:Microchemistry / Q000379:methods
D004800:Enzymes, Immobilized; D005936:Glucans; C009109:pullulan; D000687:Amylopectin; D005087:Glucan 1,4-alpha-Glucosidase