Improved quantification limits in chiral capillary electrochromatography by peak compression effects.
The peak compression effect has been applied to improve quantification limits in chiral capillary electrochromatography (CEC). A stationary phase based on the chiral selector vancomycin (Chirobiotic V) was used for separations of the enantiomers of mianserin. By adding solvents with a low dielectric constant, e.g. 2-propanol or tetrahydrofuran, to the sample solution, peak compression could be induced. The plate numbers for the minor enantiomer increased from approximately 100,000 to 1.4-1.6 million plates/m, when the composition of the mobile phase was adjusted so that the analyte eluted within either one of two system zones originating from the sample solution. A 10-fold improvement in the quantification limit for the minor enantiomer was obtained compared to elution under non-focused conditions.
Department of Analytical Chemistry, AstraZeneca, Process R&D, S-151 85 Södertälje, Sweden. anna-maria.enlund@astrazeneca.com
Anna Maria
AM
Enlund
Margareta E
ME
Andersson
Gunnar
G
Hagman
D019840:2-Propanol; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D005663:Furans; D006863:Hydrogen-Ion Concentration; D008803:Mianserin / Q000737:chemistry; D012997:Solvents; D013237:Stereoisomerism; D014640:Vancomycin / Q000737:chemistry
D002021:Buffers; D005663:Furans; D012997:Solvents; D008803:Mianserin; C018674:tetrahydrofuran; D014640:Vancomycin; D019840:2-Propanol