Selective inhibition of the growth of cancer cells by diterpenes selected with embryonic cells of Xenopus.
We used Xenopus embryo cells with a cell cycle of 20-30 min to detect inhibitory effects on cell proliferation. Inhibition of proliferation was observed when isolated embryonic cells were incubated for 16 h in a simple salt solution containing the well-known anticancer drugs 5-fluorouracil and adriamycin. In addition, three diterpene compounds isolated from the anticancer herbal medicine kansui: kansuinin B, 20-OD-ingenol Z, and 20-OD-ingenol E specifically inhibited the proliferation of isolated embryonic cells. The inhibitory compounds selected using the embryonic cells also inhibited proliferation of certain mammalian cell types.
Department of Chemistry, College of Humanities and Sciences, Nihon University, Sakurajosui, Setagaya-ku, Tokyo 156-8550, Japan. miyata@chs.nihon-u.ac.jp
Shohei
S
Miyata
Li-Yan
LY
Wang
Nai-Li
NL
Wang
Xin-Sheng
XS
Yao
Susumu
S
Kitanaka
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000187:drug effects*; D009369:Neoplasms / Q000188:drug therapy*; D014407:Tumor Cells, Cultured; D014981:Xenopus
D000970:Antineoplastic Agents; D004224:Diterpenes