Partial protection against infectious bursal disease virus through DNA-mediated vaccination with the VP2 capsid protein and chicken IL-2 genes.
Several DNA vaccination experiments were performed to determine the protective capability of a plasmid DNA molecule encoding the VP2 capsid protein gene of infectious bursal disease virus (IBDV) injected into chickens in the presence or absence of chicken interleukin 2 (IL-2) plasmid DNA. The results of these experiments indicate that partial protection against IBDV can be achieved by using the VP2 gene of IBDV as a DNA vaccine. Furthermore, the simultaneous injection of chicken IL-2 plasmid DNA significantly increased the protection after challenge with the virulent strain. It was also found that immunological tolerance may have been induced in one of the chicken experiments by vaccination with plasmid DNA.
Hulse
Diane J
DJ
Department of Pathobiology, University of Florida, 2015 SW 16th Ave. Gainesville, FL 32611, USA. diane.hulse@stjude.org
Romero
Carlos H
CH
D000818:Animals; D018175:Birnaviridae Infections / Q000276:immunology* / Q000473:pathology; D001835:Body Weight / Q000502:physiology; D002060:Bursa of Fabricius / Q000473:pathology; D036022:Capsid Proteins / Q000235:genetics* / Q000276:immunology; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics* / Q000276:immunology; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics; D005455:Fluorescent Antibody Technique; D006470:Hemorrhage / Q000473:pathology; D007243:Infectious bursal disease virus / Q000276:immunology*; D007376:Interleukin-2 / Q000235:genetics* / Q000276:immunology; D009500:Neutralization Tests; D010957:Plasmids / Q000276:immunology; D013154:Spleen / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology
D036022:Capsid Proteins; D004279:DNA, Viral; D007376:Interleukin-2; D019444:Vaccines, DNA