[Effects of adenosine preconditioning on cardiac myocyte apoptosis and expression of nuclear factor-kappaB in ischemia/reperfusion rats].
OBJECTIVE
To investigate the effect of adenosine preconditioning on cardiac myocyte apoptosis and the expression of nuclear factor-kappaB (NF-kappaB)during myocardial ischemia/reperfusion (I/R) in rats.


METHODS
A model of I/R injury (I/R group) and pretreatment with adenosine (AP group) was reproduced in rats. The morphologic features of apoptosis were identified histochemically by TdT-mediated dUTP nick end (TUNEL) staining. Expression of NF-kappaB in apoptosis myocardiac cells was observed by immunohistochemical stain and image analysis.


RESULTS
The percentage of cardiac myocyte apptosis and the expression of NF-kappaB in AP group were (2635.0+/-316.0)% and (33.21+/-16.91)%, they were significantly lower than those in I/R group (5013.0+/-503.7) % and (59.30+/-10.36) % respectively  (P<0.05 or P<0.01), but higher than those in control group (68.7+/-50.3) % and (10.98+/-4.65)% respectively (both P<0.01).


CONCLUSIONS
Adenosine preconditioning can pretect from myocardial I/R injury and relieve cardiac myocyte apoptosis and expression of NF-kappaB.
Department of Anesthesiology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong, China.
Liang-qing
LQ
Zhang
Jun-fa
JF
Xu
Kang-rong
KR
Cai
Li-zhi
LZ
Li
D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D019157:Ischemic Preconditioning, Myocardial; D008297:Male; D032383:Myocytes, Cardiac / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology; D016328:NF-kappa B / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014665:Vasodilator Agents / Q000494:pharmacology*
D016328:NF-kappa B; D014665:Vasodilator Agents; D000241:Adenosine