Ectopic expression of a c1-I allele from maize inhibits pigment formation in the flower of transgenic tobacco.
MYB-related proteins play a key role in regulating the biosynthesis of anthocyanins in plants at the transcriptional level. An MYB gene designated c1-I-2K1 (GenBank accession no. AY237128) was cloned from maize line "2K1 purple," and except for a deletion of nine nucleotides encoding three amino acids right at the carboxyl terminal end of the encoded protein, it was identical to the previously characterized c1-I gene. Flowers of transgenic tobacco overexpressing this c1-I allele showed severe reduction in pigmentation, whereas the pigmentation patterns of flowers of tobacco transformed with both c1-I-2K1 and R homologue gene r-2K1 showed no obvious change. Thus, this c1-I allele appears to act as a repressor in pigment formation and function through titration of a bHLH factor.
Life Sciences Center, College of Life Sciences, Peking University, Beijing 100871, China.
Bojun
B
Chen
Xiqing
X
Wang
Yuanlei
Y
Hu
Yong
Y
Wang
Zhongping
Z
Lin
D000483:Alleles; D000595:Amino Acid Sequence / Q000235:genetics; D000872:Anthocyanins / Q000096:biosynthesis* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D035264:Flowers / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D020598:Proto-Oncogene Proteins c-myb / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion / Q000235:genetics; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics; D003313:Zea mays / Q000235:genetics*
D000872:Anthocyanins; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D020598:Proto-Oncogene Proteins c-myb; D011994:Recombinant Proteins; D014157:Transcription Factors