Human monoclonal antibody fragment specific for glycoprotein G in herpes simplex virus type 2 with applications for serotype-specific diagnosis.
A combinatorial library was used to select a human monoclonal antibody fragment (Fab) with high affinity for G glycoprotein in herpes simplex virus type 2 (HSV-2). Tests with 112 clinical specimens demonstrated successful discrimination between HSV-2 and HSV-1, showing the potential of Fab as a low-cost tool for HSV subtyping in clinical diagnosis.
Istituto di Microbiologia, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.
Francesca
F
Bugli
Stefania
S
Manzara
Riccardo
R
Torelli
Rosalia
R
Graffeo
Rosaria
R
Santangelo
Paola
P
Cattani
Giovanni
G
Fadda
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D002522:Chlorocebus aethiops; D006561:Herpes Simplex / Q000175:diagnosis*; D018258:Herpesvirus 2, Human / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D010446:Peptide Fragments; D014759:Viral Envelope Proteins; C044525:glycoprotein B, herpes simplex virus type 2