Development and validation of a new in vitro assay for selection of probiotic bacteria that express immune-stimulating properties in chickens in vivo.
Oral administration of immunoprobiotic bacteria may support animal health. Species specificity of such microorganisms requires appropriate selection. An in vitro assay for the selection of immunoprobiotic lactic acid bacteria was developed in chicken. The assay allowed testing of large numbers of individual strains. Immune stimulation in vitro correlated well with the in vivo situation in two experiments and no false negative results occurred. Therefore this assay is an appropriate selection tool for immunomodulating properties of lactic acid bacteria in chicken.
Koenen
Marjorie E
ME
Animal Sciences Group, Wageningen UR, Division of Animal Resources Development, P.O. Box 65, 8200 AB Lelystad, The Netherlands. marjorie.koenen@wur.nl
van der Hulst
Remy
R
Leering
Mariska
M
Jeurissen
Suzan H M
SH
Boersma
Wim J A
WJ
D000818:Animals; D001681:Biological Assay; D002645:Chickens / Q000276:immunology / Q000382:microbiology*; D066298:In Vitro Techniques; D007778:Lactobacillus / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D019936:Probiotics / Q000008:administration & dosage* / Q000302:isolation & purification