Trypanosoma cruzi DNA in cardiac lesions of Argentinean patients with end-stage chronic chagas heart disease.
The extent of inflammation, fibrosis, and progression of chronic Chagas heart disease (cChHD) was associated with persistence of parasite DNA in cardiac lesions of necropsies or explants from Argentinean cChHD patients. A Trypanosoma cruzi-based polymerase chain reaction showed a positive result in 1) 15% of cardiac sections with less than 10 mononuclear inflammatory cells/high-power field (440x) (MNC/HPF), 89% with 10-19 MNC/HPF, and 100% with more than 20 MNC/HPF (P < 0.0001); 2) 33% with less than 10% fibrosis, 79% with 10-19% fibrosis, and 100% with more than 20% fibrosis (P < 0.01); 3) 25% of specimens from patients classified in Kuschnir groups 0 and I, 70% in group II and 90% in group III (P < .001); and 4) 45% and 90% of the specimens from cChHD patients without or with heart failure, respectively (P < 0.01). These findings stress the role of the parasite in pathogenesis and disease progression of cChHD.
Laboratorio de Biología Molecular de la Enfermedad de Chagas, INGEBI, Consejo Nacional de Ciencia y Tecnologia, Department of Fisiology, Celular and Molecular Biology, F. CEyN, University of Buenos Aires, Buenos Aires, Argentina.
Alejandro G
AG
Schijman
Carlos A
CA
Vigliano
Rodolfo J
RJ
Viotti
Juan M
JM
Burgos
Silvia
S
Brandariz
Bruno E
BE
Lococo
Maria I
MI
Leze
Hector A
HA
Armenti
Mariano J
MJ
Levin
D000328:Adult; D000368:Aged; D000818:Animals; D001118:Argentina; D001483:Base Sequence; D015139:Blotting, Southern; D002598:Chagas Cardiomyopathy / Q000401:mortality / Q000469:parasitology* / Q000473:pathology; D002908:Chronic Disease; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D005260:Female; D005355:Fibrosis; D006321:Heart / Q000469:parasitology*; D006801:Humans; D007249:Inflammation; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009206:Myocardium / Q000473:pathology*; D016133:Polymerase Chain Reaction; D014349:Trypanosoma cruzi / Q000235:genetics / Q000302:isolation & purification*
D016054:DNA, Protozoan