Mitotic phosphorylation of histone H3 at threonine 3.
Nuclear envelope-peripheral heterochromatin fractions contain multiple histone kinase activities. In vitro assays and amino-terminal sequencing show that one of these activities co-isolates with heterochromatin protein 1 (HP1) and phosphorylates histone H3 at threonine 3. Antibodies recognizing this post-translational modification reveal that in vivo phosphorylation at threonine 3 commences at early prophase in the vicinity of the nuclear envelope, spreads to pericentromeric chromatin during prometaphase and is fully reversed by late anaphase. This spatio-temporal pattern is distinct from H3 phosphorylation at serine 10, which also occurs during cell division, suggesting segregation of differentially phosphorylated chromatin to different regions of mitotic chromosomes.
Polioudaki
Hara
H
Department of Basic Sciences, The University of Crete, School of Medicine, 95110 Heraklion, Crete, Greece.
Markaki
Yolanda
Y
Kourmouli
Niki
N
Dialynas
George
G
Theodoropoulos
Panayiotis A
PA
Singh
Prim B
PB
Georgatos
Spyros D
SD
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000737:chemistry; D004910:Erythrocyte Membrane / Q000737:chemistry; D004912:Erythrocytes / Q000166:cytology; D005982:Glutathione Transferase / Q000378:metabolism; D006570:Heterochromatin / Q000737:chemistry; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D008938:Mitosis; D009685:Nuclear Envelope / Q000737:chemistry; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D013912:Threonine / Q000378:metabolism*; D014422:Turkeys / Q000097:blood
D006570:Heterochromatin; D006657:Histones; D011993:Recombinant Fusion Proteins; D013912:Threonine; D005982:Glutathione Transferase