An in vivo model of ischaemia-reperfusion injury and ischaemic preconditioning in the mouse heart.
BACKGROUND
Our aim was to develop a robust protocol for inducing ischaemia-reperfusion injury and preconditioning in the in vivo mouse heart.


METHODS
Animals were prepared by standard techniques adapted for the mouse.


RESULTS
Thirty minutes of ischaemia appeared to strike the most appropriate balance between large infarct volumes with the risk of heart failure and excessively small infarcts. With this duration of ischaemia, a protocol of three sets of 5 min of ischaemia and 5 min of reperfusion was found to precondition, leading to significantly smaller infarct sizes (infarct volume: risk zone volume reduced to 10.1 +/- 2.7% from 41.39% +/- 3.0; P < .05, n = 6 and 7, respectively). Late preconditioning also occurred (infarct volume: risk zone volume = 57.9 +/- 10.1% vs. 35.4 +/- 4.2%, sham compared to late preconditioning, respectively; P = .001, n = 7).


CONCLUSIONS
Whilst further refinements may indeed be possible, we feel this article details a valuable, robust protocol for in vivo studies of ischaemia-reperfusion injury and preconditioning in the mouse heart.
Fisher
Simon G
SG
Department of Cardiology, Division of Cardiovascular Science, GKT School of Medicine, King's College London, The Rayne Institute, St. Thomas' Hospital, Lambeth Palace Road, London SE1 7EH, UK. s.fisher@rfc.ucl.ac.uk
Marber
Michael S
MS
D000818:Animals; D001794:Blood Pressure; D006321:Heart / Q000503:physiopathology*; D019194:Ischemic Preconditioning; D008026:Ligation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008953:Models, Anatomic; D017202:Myocardial Ischemia / Q000209:etiology / Q000503:physiopathology*; D015428:Myocardial Reperfusion Injury / Q000503:physiopathology*; D010865:Pilot Projects; D013997:Time Factors