Growth characteristics and eicosapentaenoic acid production by Nannochloropsis sp. in mixotrophic conditions.
Nannochloropsis sp. was grown under mixotrophic conditions with 30 mM glucose as carbon source, reaching 507 mg dry wt l(-1) after 8 d. This was 1.4-fold of that obtained under photoautotrophic conditions. Under mixotrophic and photoautotrophic cultivations, the net photosynthetic rate of Nannochloropsis sp. did not change but the respiratory rate increased in the mixotrophic cultivation. The yield of eicosapentaenoic acid was 22 mg l(-1) in the mixotrophic cultivation and 20 mg l(-1) under the photoautotrophic cultivation.
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, PO. Box 353, PR China. xufang1974@yahoo.com
Fang
F
Xu
Han-hua
HH
Hu
Wei
W
Cong
Zhao-ling
ZL
Cai
Fan
F
Ouyang
D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000528:radiation effects; D015118:Eicosapentaenoic Acid / Q000096:biosynthesis*; D056890:Eukaryota / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism* / Q000528:radiation effects; D008027:Light; D010788:Photosynthesis / Q000502:physiology*
D015118:Eicosapentaenoic Acid