Hepatocurative and antioxidant profile of HP-1, a polyherbal phytomedicine.
HP-1 a herbal formulation comprising of Phyllanthus niruri and extracts of Terminalia belerica, Terminalia chebula, Phyllanthus emblica and Tinospora cordifolia has been evaluated for hepatoprotective activity against carbon tetrachloride (CCl4) induced toxicity. Results show that HP-1 reversed the leakage of lactate dehydrogenase (LDH) and glutamate pyruvate transaminase (GPT) and prevented the depletion of glutathione (GSH) levels in a primary monolayer culture of rat hepatocytes (in vitro). HP-1 attenuated the serum toxicity as manifested in elevated levels of transaminases (glutamate oxaloacetate transaminase (GOT), and GPT) The antioxidative enzymes in liver (catalase and superoxide dismutase (SOD)) were restored to normal values after the oral administration of HP-1. HP-1 suppressed the formation of the superoxide anion radical and reduced CCl4 mediated lipid peroxidation (LPO). Silymarin and antioxidants (ascorbic acid, beta-carotene and alpha-tocopherol) were used for comparison. The present study showed that HP-1 is a potential hepatoprotective formulation with an additional attribute of being anti-peroxidative.
Biochemistry Lab, Division of Pharmacology, Regional Research Laboratory, Canal Road, Jammu-Tawi 180 001, India.
S A
SA
Tasaduq
K
K
Singh
S
S
Sethi
S C
SC
Sharma
K L
KL
Bedi
J
J
Singh
B S
BS
Jaggi
R K
RK
Johri
D000284:Administration, Oral; D000410:Alanine Transaminase / Q000502:physiology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000494:pharmacology; D002251:Carbon Tetrachloride / Q000008:administration & dosage / Q000009:adverse effects / Q000037:antagonists & inhibitors; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy*; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D004338:Drug Combinations; D005562:Formazans / Q000378:metabolism; D005978:Glutathione / Q000502:physiology; D022781:Hepatocytes / Q000187:drug effects / Q000201:enzymology; D007194:India; D007770:L-Lactate Dehydrogenase / Q000502:physiology; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D008297:Male; D029803:Phyllanthus; D008517:Phytotherapy; D028321:Plant Preparations / Q000494:pharmacology / Q000627:therapeutic use; D010946:Plants, Medicinal / Q000737:chemistry / Q000187:drug effects; D051381:Rats; D012838:Silymarin / Q000008:administration & dosage / Q000494:pharmacology; D029766:Terminalia; D031610:Tinospora; D024502:alpha-Tocopherol / Q000008:administration & dosage / Q000494:pharmacology; D019207:beta Carotene / Q000008:administration & dosage / Q000494:pharmacology
D000975:Antioxidants; D004338:Drug Combinations; D005562:Formazans; D028321:Plant Preparations; D012838:Silymarin; D019207:beta Carotene; D002251:Carbon Tetrachloride; D007770:L-Lactate Dehydrogenase; D000410:Alanine Transaminase; D005978:Glutathione; D024502:alpha-Tocopherol; D001205:Ascorbic Acid