Electrochemical investigations of baicalin and DNA-baicalin interactions.
Baicalin is an anti-HIV drug purified from the traditional Chinese medicinal plant Scutellaria Baicalensis Georgi. Baicalin has proven to be electroactive at pyrolytic graphite (PG) electrodes. We thus studied its interaction with DNA via the electrochemical approach. We observed that the peak currents corresponding to the baicalin reduction-oxidation (redox) reaction significantly decrease upon the addition of DNA. With complementary ultraviolet/visible (UV/Vis) spectroscopic evidence, we suggest that baicalin binds to DNA through intercalation. This feature has enabled baicalin to discriminate between double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA).
Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, 210093, Nanjing, China.
Ziyi
Z
Sun
Zhong
Z
Ma
Wenjun
W
Zhang
Xiaoyue
X
Wang
Chunhai
C
Fan
Genxi
G
Li
D000818:Animals; D019380:Anti-HIV Agents / Q000737:chemistry* / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D005419:Flavonoids / Q000737:chemistry* / Q000378:metabolism
D019380:Anti-HIV Agents; D005419:Flavonoids; C038044:baicalin; D004247:DNA