[Experimental study on the activity of Staphyloccocal enterotoxin A liposome for inducing cytotoxicity of TIL from human hepatocellular carcinoma against tumor cells].
OBJECTIVE
To investigate the activity of Staphyloccocal enterotoxin A liposome (L-SEA) for inducing cytotoxicity of tumor infiltrating lymphocytes (TIL) against tumor cells.


METHODS
TIL were isolated from the tumor tissues of five hepatocellular carcinoma patients. L-SEA, SEA and IL-2 were tested in vitro for their activity levels in stimulating TIL proliferation. The TNF-alpha and IFN-gamma secretion and cytotoxicity of TIL against HepG-2 liver cancer cells were estimated by ELISA and MTT, respectively.


RESULTS
Both L-SEA and SEA significantly stimulated the proliferation of TIL. The cytokine secretion of L-SEA group was significantly higher than that of IL-2 group (P < 0.05). There was no significantly statistical difference in cytokine secretion between L-SEA group and SEA group (P > 0.05) except that IFN-gamma secretion of L-SEA group was lower than that of SEA group at day 4 (P < 0.05). Both L-SEA and SEA had potent ability to induce TIL cytotoxicity against HepG-2 cells. And no significant difference was observed between these two groups (P > 0.05).


CONCLUSIONS
These results suggest that L-SEA is as efficient as SEA in activating TIL.
Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Mao-de
MD
Li
Zhi-yu
ZY
Li
Sheng
S
He
Hua
H
Xue
D000328:Adult; D016516:CD4-CD8 Ratio; D006528:Carcinoma, Hepatocellular / Q000276:immunology / Q000473:pathology; D002469:Cell Separation; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D004768:Enterotoxins / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D008081:Liposomes / Q000494:pharmacology; D008113:Liver Neoplasms / Q000276:immunology / Q000473:pathology*; D016246:Lymphocytes, Tumor-Infiltrating / Q000166:cytology / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D004768:Enterotoxins; D007376:Interleukin-2; D008081:Liposomes; D014409:Tumor Necrosis Factor-alpha; C026066:enterotoxin A, Staphylococcal