Anti-ulcerogenic effect of chitin and chitosan on mucosal antioxidant defence system in HCl-ethanol-induced ulcer in rats.
The anti-ulcerogenic effect of chitin and chitosan against ulcer induced by HCl-ethanol in male Wistar rats was studied. Levels of acid output, pepsin, protein, lipid peroxides and reduced glutathione and the activity of glutathione peroxidase (GPx), glutathione-S-transferase (GST), catalase (CAT) and superoxide dismutase (SOD) were determined in the gastric mucosa of normal and experimental groups of rats. A significant increase in volume and acidity of the gastric juice was observed in the ulcer-induced group of rats. Peptic activity was significantly decreased as compared with that of normal controls. In the rats pre-treated with chitin and chitosan 2% along with feed, the volume and acid output and peptic activity of gastric mucosa were maintained at near normal levels. The level of lipid peroxidation was significantly higher in the ulcerated mucosa when compared with that of normal controls. This was paralleled by a decline in the level of reduced glutathione and in the activity of antioxidant enzymes like GPx, GST, CAT and SOD in the gastric mucosa of ulcer-induced rats. Also, the levels of mucosal proteins and glycoprotein components were significantly depleted in ulcerated mucosa. The pre-treatment with chitin and chitosan was found to exert a significant anti-ulcer effect by preventing all the HCl-ethanol-induced ulcerogenic effects in experimental rats.
Biochemistry and Nutrition Division, Central Institute of Fisheries Technology, Matsyapuri (PO), Cochin-682029, India. krandandan@email.com
R
R
Anandan
P G Viswanathan
PG
Nair
Suseela
S
Mathew
D000818:Animals; D000897:Anti-Ulcer Agents / Q000493:pharmacokinetics / Q000627:therapeutic use*; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000096:biosynthesis / Q000737:chemistry / Q000502:physiology; D002686:Chitin / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000627:therapeutic use*; D048271:Chitosan; D000431:Ethanol / Q000008:administration & dosage / Q000009:adverse effects*; D005750:Gastric Juice / Q000187:drug effects / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D005978:Glutathione / Q000096:biosynthesis / Q000737:chemistry / Q000502:physiology; D005979:Glutathione Peroxidase / Q000096:biosynthesis / Q000737:chemistry / Q000502:physiology; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000737:chemistry / Q000502:physiology; D006851:Hydrochloric Acid / Q000008:administration & dosage / Q000009:adverse effects*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007194:India; D008054:Lipid Peroxides / Q000096:biosynthesis / Q000737:chemistry / Q000502:physiology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced* / Q000188:drug therapy; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000737:chemistry / Q000502:physiology
D000897:Anti-Ulcer Agents; D000975:Antioxidants; D008054:Lipid Peroxides; D002686:Chitin; D000431:Ethanol; D048271:Chitosan; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D005978:Glutathione; D006851:Hydrochloric Acid