Chromosomal localization of sixty autosomal loci in sheep (OVIS ARIES, 2n = 54) by fluorescence in situ hybridization and R-banding.
Sixty autosomal loci (5 type I and 55 type II) from 24 bovine syntenic groups, and previously FISH-mapped to goat and river buffalo chromosomes, were localized by fluorescence in situ on sheep (OVIS ARIES, 2n = 54) chromosomes, thereby notably extending the cytogenetic map of this economically important species. Caprine BAC clones were hybridized to R-banded chromosome preparations. FITC-signals and RBPI- banding (R-banding by late BrdU-incorporation and propidium iodide staining) were simultaneously visualized and captured by a colour CCD-camera. All mapped loci were localized on homoeologous chromosomes and chromosome regions (bands) of sheep, goat and river buffalo, further supporting chromosome and genetic (loci) homoeologies among bovids.
Iannuzzi
L
L
National Research Council (CNR), ISPAAM, Laboratory of Animal Cytogenetics and Gene Mapping, Naples, Italy. L.Iannuzzi@iabbam.na.cnr.it
Perucatti
A
A
Di Meo
G P
GP
Schibler
L
L
Incarnato
D
D
Cribiu
E P
EP
D000818:Animals; D002417:Cattle; D002871:Chromosome Banding; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian; D017404:In Situ Hybridization, Fluorescence; D034561:Sheep, Domestic / Q000235:genetics*; D026801:Synteny