Lipase-catalyzed esterification of palm-based 9,10-dihydroxystearic acid and 1-octanol in hexane--a kinetic study.
The esterification of palm-based 9,10-dihydroxystearic acid (DHSA) and 1-octanol in hexane as catalyzed by lipase from Rhizomucor meihei (Lipozyme IM) followed Michaelis-Menten kinetics. The esterification reaction follows a Ping-Pong, Bi-Bi mechanism. The maximum rate was estimated to be 1 micromol min(-1) mg(-1) catalyst in hexane at 50 degrees C, and the Michaelis-Menten constants for DHSA and 1-octanol were 1.3 M and 0.7 M, respectively.
Advanced Oleochemical Technology Centre (AOTC), Malaysian Palm Oil Board, Lot 9 & 11, Jalan P/14, Section 10, 43650 Bandar Baru Bangi, Selangor, Malaysia. roila@mpob.gov.my
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Awang
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Basri
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Ahmad
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Salleh
D002384:Catalysis; D004789:Enzyme Activation; D004951:Esterification; D004952:Esters; D006586:Hexanes / Q000737:chemistry*; D007700:Kinetics; D008049:Lipase / Q000737:chemistry*; D008956:Models, Chemical; D000442:Octanols / Q000737:chemistry*; D000073878:Palm Oil; D010938:Plant Oils / Q000737:chemistry*; D013229:Stearic Acids / Q000737:chemistry*
D004952:Esters; D006586:Hexanes; D000442:Octanols; D010938:Plant Oils; D013229:Stearic Acids; C074110:9,10-dihydroxystearic acid; D000073878:Palm Oil; C093666:Lipozyme; D008049:Lipase