Three Arabidopsis MBF1 homologs with distinct expression profiles play roles as transcriptional co-activators.
Multiprotein bridging factor 1 (MBF1) is known to be a transcriptional co-activator that mediates transcriptional activation by bridging between an activator and a TATA-box binding protein (TBP). We demonstrated that expression of every three MBF1 from Arabidopsis partially rescues the yeast mbf1 mutant phenotype, indicating that all of them function as co-activators for GCN4-dependent transcriptional activation. We also report that each of their subtypes shows distinct tissue-specific expression patterns and responses to phytohormones. These observations suggest that even though they share a similar biochemical function, each MBF1 has distinct roles in various tissues and conditions.
Tsuda
Kenichi
K
Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Kita 10, Nishi 5, Kita-ku, Sapporo, 060-0810 Japan.
Tsuji
Toshihiro
T
Hirose
Susumu
S
Yamazaki
Ken-ichi
K
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D050976:Basic-Leucine Zipper Transcription Factors; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D005982:Glutathione Transferase / Q000378:metabolism; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology; D010940:Plant Proteins; D011485:Protein Binding / Q000235:genetics; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D035181:TATA-Box Binding Protein / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation / Q000235:genetics*
C489413:AHBP-1b protein, Arabidopsis; D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C491466:MBF1 protein, Arabidopsis; C106951:MBF1 protein, S cerevisiae; D046911:Macromolecular Substances; D009687:Nuclear Proteins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D035181:TATA-Box Binding Protein; D015534:Trans-Activators; D014157:Transcription Factors; C068757:HBP-1b protein, plant; D005982:Glutathione Transferase; D011494:Protein Kinases