Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX.
We produced a novel cationic-charged modified DNA aptamer for sialyllactose that is a ubiquitous component of the cell surface responsible for the infection of several viruses by using the magnetic-particle-based SELEX method. After 13 rounds of selection we selected 22 clones as sialyllactose-binding DNA aptamers composed of several modified thymidines. The DNA aptamers could form a three-way junction structure that likely forms a binding site for siallyllactose. The three-way junction structure contains several modified thymidines bearing a positively-charged amino group at the C5 position, which could enhance the binding ability for silalyllactose which has a negatively-charged carboxyl group. The dissociation constant of the aptamer that showed the strongest sialyllactose-binding ability among the clones of the aptamers was 4.9 microM.
Masud
Mohammad Mehedi
MM
Department of Applied Chemistry, Gunma University, Kiryu, Gunma 376-8515 Japan.
Kuwahara
Masayasu
M
Ozaki
Hiroaki
H
Sawai
Hiroaki
H
D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D015723:Gene Library; D007785:Lactose / Q000031:analogs & derivatives* / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors / Q000737:chemistry; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D013936:Thymidine / Q000031:analogs & derivatives / Q000737:chemistry
C426347:2,6-sialyllactose; D008562:Membrane Glycoproteins; D004247:DNA; D007785:Lactose; D013936:Thymidine