Cell-to-Cell, but not long-distance, spread of RNA silencing that is induced in individual epidermal cells.
A Turnip crinkle virus (TCV)-based system was devised to discriminate cell-to-cell and systemic long-distance spread of RNA silencing in plants. Modified TCV-GFPDeltaCP, constructed by replacing the coat protein (CP) gene with the green fluorescent protein (GFP) gene, replicated in single epidermal cells but failed to move from cell to cell in Nicotiana benthamiana. Mechanical inoculation of TCV-GFPDeltaCP induced effective RNA silencing in single epidermal cells which spread from cell to cell to form silenced foci on inoculated leaves, but no long-distance systemic spread of RNA silencing occurred. Agroinfiltration of TCV-GFPDeltaCP was, however, able to induce both local and systemic RNA silencing. TCV coinfection arrested TCV-GFPDeltaCP-mediated local induction of RNA silencing. Possible mechanisms involved in cell-to-cell and long-distance spread of RNA silencing are discussed.
Horticulture Research International, East Malling, West Malling, Kent ME19 6BJ, United Kingdom. eugene.ryabov@bri.ac.uk
Eugene V
EV
Ryabov
Rene
R
van Wezel
John
J
Walsh
Yiguo
Y
Hong
D029688:Brassica napus / Q000821:virology*; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D019184:Carmovirus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D034622:RNA Interference; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000821:virology
D036022:Capsid Proteins; D008164:Luminescent Proteins; D012367:RNA, Viral; D049452:Green Fluorescent Proteins