Application of new in situ hybridization probes for Ku70 and Ku80 in tissue microarrays of paraffin-embedded malignant melanomas: correlation with immunohistochemical analysis.
Ku70 and Ku80 proteins are responsible for the repair of DNA double-strand breaks and function as a regulatory subunit of the DNA-dependent protein kinase. In this study we analyzed expression of both genes in malignant melanoma tissue arrays applying in situ hybridization probes produced by our research group and using immunohistochemical analysis. Expression of both genes was down-regulated as melanoma progressed. In situ hybridization demonstrated more Ku70- and Ku80-positive cells than immunohistochemical methods, but the correlation between the two methods was highly significant (P <0.01). We conclude that the in situ hybridization assay for the detection of Ku70 and Ku80 expression used in this study is also suitable for tissue microarray analysis of paraffin-embedded melanoma samples. The laboratory procedure is much more complicated than the immunohistochemical method, however.
Department of Cytopathology, Georg-August-University, Goettingen, Germany.
Monika
M
Korabiowska
Hanne
H
Bauer
Tomas
T
Quentin
Jerzy
J
Stachura
Carlos
C
Cordon-Cardo
Ulrich
U
Brinck
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D034961:Antigens, Nuclear / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D004265:DNA Helicases; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000032:analysis*; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization / Q000379:methods*; D000072200:Ku Autoantigen; D008297:Male; D008545:Melanoma / Q000737:chemistry* / Q000235:genetics / Q000473:pathology*; D008875:Middle Aged; D009367:Neoplasm Staging; D010232:Paraffin; D016133:Polymerase Chain Reaction; D040081:Protein Array Analysis; D015347:RNA Probes; D018075:RNA, Complementary / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D012878:Skin Neoplasms / Q000737:chemistry / Q000473:pathology
D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D015347:RNA Probes; D018075:RNA, Complementary; D012334:RNA, Neoplasm; D010232:Paraffin; D004265:DNA Helicases; C106893:XRCC5 protein, human; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen