Fate of multipotent neural precursor cells transplanted into mouse retina selectively depleted of retinal ganglion cells.
In some parts of the CNS, depletion of a particular class of neuron might induce changes in the microenvironment that influence the differentiation of newly grafted neural precursor cells. This hypothesis was tested in the retina by inducing apoptotic retinal ganglion cell (RGC) death in neonatal and adult female mice and examining whether intravitreally grafted male neural precursor cells (C17.2), a neural stem cell (NSC)-like clonal line, become incorporated into these selectively depleted retinae. In neonates, rapid RGC death was induced by removal of the contralateral superior colliculus (SC), in adults, delayed RGC death was induced by unilateral optic nerve (ON) transection. Cells were injected intravitreally 6-48 h after SC ablation (neonates) or 0-7 days after ON injury (adults). Cells were also injected into non-RGC depleted neonatal and adult retinae. At 4 or 8 weeks, transplanted cells were identified using a Y-chromosome marker and in situ hybridisation or by their expression of the lacZ reporter gene product Escherichia coli beta-galactosidase (beta-gal). No C17.2 cells were identified in axotomised adult-injected eyes undergoing delayed RGC apoptosis (n = 16). Donor cells were however stably integrated within the retina in 29% (15/55) of mice that received C17.2 cell injections 24 h after neonatal SC ablation; 6-31% of surviving cells were found in the RGC layer (GCL). These NSC-like cells were also present in intact retinae, but on average, there were fewer cells in GCL. In SC-ablated mice, most grafted cells did not express retinal-specific markers, although occasional donor cells in the GCL were immunopositive for beta-III tubulin, a protein highly expressed by, but not specific to, developing RGCs. Targeted rapid RGC depletion thus increased cell incorporation into the GCL, but grafted C17.2 cells did not appear to differentiate into an RGC phenotype.
School of Anatomy and Human Biology, West Australian Institute for Medical Research, The University of Western Australia, Crawley, Perth, WA, Australia.
Carla B
CB
Mellough
Qi
Q
Cui
Kirsty L
KL
Spalding
Natalie A
NA
Symons
Margaret A
MA
Pollett
Evan Y
EY
Snyder
Jeffrey D
JD
Macklis
Alan R
AR
Harvey
D000818:Animals; D051379:Mice; D000831:Animals, Newborn; D017209:Apoptosis / Q000502:physiology; D044963:Biopsy, Fine-Needle; D064026:Calbindins; D002452:Cell Count; D002470:Cell Survival; D005697:Galactosides / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007211:Indoles / Q000378:metabolism; D008807:Mice, Inbred BALB C; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D016900:Neurofilament Proteins / Q000378:metabolism; D009474:Neurons / Q000637:transplantation; D020221:Optic Nerve Injuries / Q000503:physiopathology; D010320:Parvalbumins / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D051571:Protein Kinase C-alpha; D012160:Retina / Q000166:cytology; D012165:Retinal Ganglion Cells / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D033581:Stem Cell Transplantation / Q000379:methods; D013234:Stem Cells / Q000378:metabolism; D013477:Superior Colliculi / Q000502:physiology / Q000601:surgery; D014404:Tubulin / Q000378:metabolism; D014998:Y Chromosome / Q000235:genetics / Q000378:metabolism
C044888:5-bromo-4-chloro-3-indolyl beta-galactoside; C089138:beta-galactanase; C462941:beta3 tubulin, mouse; D064026:Calbindins; D005697:Galactosides; D005904:Glial Fibrillary Acidic Protein; D006026:Glycoside Hydrolases; D007211:Indoles; D008869:Microtubule-Associated Proteins; C051774:neurofilament protein H; C066858:neurofilament protein NF 68; D016900:Neurofilament Proteins; D010320:Parvalbumins; C496630:Prkca protein, mouse; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D064030:S100 Calcium Binding Protein G; D014404:Tubulin; C000721970:MAP7 protein, mouse