Bioimprinted QCM sensors for virus detection-screening of plant sap.
Surface imprinting techniques on polymer-coated quartz-crystal microbalances (QCM) have been used to detect tobacco mosaic viruses (TMV) in aqueous media. Molecularly imprinted polymers (MIP), tailor-made by self organisation of monomers around a template (TMV), were generated directly on the gold electrodes. Imprinted trenches on the polymer surface mimicking the shape and surface functionality of the virus serve as recognition sites for re-adsorption after washing out of the template. The sensors are applicable to TMV detection ranging from 100 ng mL(-1) to 1 mg mL(-1) within minutes. Furthermore, direct measurements without time-consuming sample preparation are possible in complex matrices such as tobacco plant sap.
Institute of Analytical Chemistry, Vienna University, Waehringerstrasse 38, 1090 Vienna, Austria. Franz.Dickert@univie.ac.at
Franz L
FL
Dickert
Oliver
O
Hayden
Roland
R
Bindeus
Karl-J
KJ
Mann
Dieter
D
Blaas
Elisabeth
E
Waigmann
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D001826:Body Fluids / Q000821:virology; D018625:Microscopy, Atomic Force; D018515:Plant Leaves / Q000737:chemistry / Q000821:virology; D018514:Plant Structures / Q000821:virology*; D011140:Polyurethanes / Q000737:chemistry; D011791:Quartz; D012680:Sensitivity and Specificity; D013997:Time Factors; D014026:Nicotiana / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000302:isolation & purification*; D014894:Weights and Measures
D011140:Polyurethanes; D011791:Quartz