[Image analysis of cardiac muscle cytoskeleton under simulated microgravity based on gray-level co-occurrence matrix (GLCM)].
OBJECTIVE
To study morphological changes of the cytoskeleton-microtubule (MT) of the fetal rat cardiac myocytes under simulated microgravity, and to quantify its image by utilizing the gray level co-occurrence matrix (GLCM) parameters of the image.


METHODS
Cytoskeleton images, including cellular microphotographs taken under normal or microgravity (clinostat) conditions, were quantified by gray level co-occurrence matrix parameters, and the pharmacological counter effect of quercetin against the influences of microgravity was estimated with these parameters.


RESULTS
The results showed that the texture of microtubules in the image became worse under simulated microgravity environment. It also showed that quercetin has certain counter effect against the influence of microgravity.


CONCLUSIONS
The microtubule of the cardiac myocytes cytoskeleton becomes diffused under microgravity, and the GLCM parameters can well describe these variation. Quercetin has certain counter-effect against the influence of microgravity.
Yao
Yu-hua
YH
Institute of Space Medico-Engineering, Beijing, China.
Yan
Hong
H
Xiong
Jiang-hui
JH
D000818:Animals; D005318:Fetal Heart / Q000166:cytology / Q000648:ultrastructure; D007090:Image Interpretation, Computer-Assisted; D008870:Microtubules / Q000648:ultrastructure*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000648:ultrastructure*; D011794:Quercetin / Q000494:pharmacology; D051381:Rats; D012399:Rotation / Q000009:adverse effects; D018544:Weightlessness Countermeasures; D018474:Weightlessness Simulation / Q000009:adverse effects
D011794:Quercetin