Comparison of different probe-level analysis techniques for oligonucleotide microarrays.
Three different software packages for the probe-level analysis of high-density oligonucleotide microarray data were compared using an experiment-derived data set that was validated using real-time PCR. The efficiency with which these three programs could identify true positives in this data set was assessed. In addition, estimates of false-positive and false-negative rates were determined. The performance of the programs using very small data sets was also compared, and recommendations for use are suggested.
Rosati
Barbara
B
State University of New York at Stony Brook, Stony Brook, NY, USA.
Grau
Frederic
F
Kuehler
Anneke
A
Rodriguez
Samantha
S
McKinnon
David
D
D000818:Animals; D005188:False Negative Reactions; D005189:False Positive Reactions; D020869:Gene Expression Profiling / Q000379:methods; D009206:Myocardium / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats
D015345:Oligonucleotide Probes