Telepathology using immunofluorescence/immunoperoxidase microscopy.
We evaluated low-cost, store-and-forward telepathology interpretation of digital images of skin sections stained immunohistochemically, using immunofluorescence (IF) and immunoperoxidase (IP). The sample comprised 17 patients with skin diseases characterized by cutaneous deposition of immunoglobulins, fibrinogen or complement components. Up to 11 digital IF or IP images (median 3) were transferred via email to centres in Graz, Austria, and Kurume, Japan. Both remote centres had expertise in reading immunohistochemical specimens. Although image files were relatively small (approximately 100 kByte), the IF images were of high quality and they were well suited to static telepathology. There was agreement on the diagnoses made by the local and both remote centres by physicians experienced in IF/IP microscopy in 14 of 17 cases (82%). These results suggest that telepathology evaluation of immunohistochemical specimens may be a useful procedure for the discussion of unusual skin disorders, training purposes and second-opinion consultations on difficult cases from centres of excellence in immunohistochemical diagnosis.
Department of Dermatology, Paracelsus Private Medical University, Salzburg, Austria.
C M
CM
Lanschuetzer
G
G
Pohla-Gubo
B
B
Schafleitner
R
R
Hametner
T
T
Hashimoto
W
W
Salmhofer
J W
JW
Bauer
H
H
Hintner
D001317:Austria; D006801:Humans; D007124:Immunoenzyme Techniques; D020407:Internet; D008856:Microscopy, Fluorescence; D012871:Skin Diseases / Q000473:pathology*; D019113:Telepathology / Q000295:instrumentation / Q000379:methods*