Dependence of the expression of the radiation-induced gene conversion to arginine independence in diploid yeast on the amino acid concentration: effect on allelic mapping.
The yield of radiation-induced gene conversion to arginine independence in diploid yeast depended on the concentration of the amino acid both in the plating medium and in the intracellular pool. By depletion of the level of arginine in the intracellular pool of amino acid or by provision of arginine at 0.4 mg/l of the plating medium the yield was varied by a factor as high as 20. This may be important in studies of the genetic mapping of alleles based on the slope of conversion frequency versus dose line.
Murthy
M S
MS
Division of Radiological Protection, Bhabha Atomic Research Centre, Trombay, Bombay-400 085, India.
Rao
B S
BS
Deorukhakar
V V
VV
D000596:Amino Acids / Q000378:metabolism*; D001120:Arginine / Q000378:metabolism*; D002874:Chromosome Mapping / Q000379:methods; D004171:Diploidy; D005785:Gene Conversion / Q000528:radiation effects*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000528:radiation effects*
D000596:Amino Acids; D001120:Arginine