Micro- and nanofluidics for DNA analysis.
Miniaturization to the micrometer and nanometer scale opens up the possibility to probe biology on a length scale where fundamental biological processes take place, such as the epigenetic and genetic control of single cells. To study single cells the necessary devices need to be integrated on a single chip; and, to access the relevant length scales, the devices need to be designed with feature sizes of a few nanometers up to several micrometers. We will give a few examples from the literature and from our own research in the field of miniaturized chip-based devices for DNA analysis, including dielectrophoresis for purification of DNA, artificial gel structures for rapid DNA separation, and nanofluidic channels for direct visualization of single DNA molecules.
Tegenfeldt
Jonas O
JO
Department of Molecular Biology, Princeton University, Princeton, NJ, USA. jonas.tegenfeldt@ftf.lth.se
Prinz
Christelle
C
Cao
Han
H
Huang
Richard L
RL
Austin
Robert H
RH
Chou
Stephen Y
SY
Cox
Edward C
EC
Sturm
James C
JC
D002469:Cell Separation; D004247:DNA / Q000032:analysis* / Q000235:genetics; D044085:Microfluidics; D036103:Nanotechnology
D004247:DNA