Fluorescent in situ hybridization to ascidian chromosomes.
The draft genome of the ascidian Ciona intestinalis has been sequenced. Mapping of the genome sequence to the Ciona 14 haploid chromosomes is essential for future studies of the genome-wide control of gene expression in this basal chordate. Here we describe an efficient protocol for fluorescent in situ hybridization for mapping genes to the Ciona chromosomes. We demonstrate how the locations of two BAC clones can be mapped relative to each other. We also show that this method is efficient for coupling two so-far independent scaffolds into one longer scaffold when two BAC clones represent sequences located at either end of the two scaffolds.
Shoguchi
Eiichi
E
Department of Zoology, Graduate School of Science, Kyoto University, Japan. eiichi@ascidian.zool.kyoto-u.ac.jp
Ikuta
Tetsuro
T
Yoshizaki
Fumiko
F
Satou
Yutaka
Y
Satoh
Nori
N
Asano
Katsutoshi
K
Saiga
Hidetoshi
H
Nishikata
Takahito
T
D000818:Animals; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D002938:Ciona intestinalis / Q000235:genetics*; D015342:DNA Probes; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*
D015342:DNA Probes