A major allergen from pollen defines a novel family of plant proteins and shows intra- and interspecies [correction of interspecie] cross-reactivity.
Olive tree (Olea europaea) pollen is a main cause of allergy associated with extensive areas of Europe and North America. Ole e 10, a small (10.8 kDa) and acidic (pI 5.8) protein, has been identified as a major allergen from the olive pollen, isolated, and characterized. Circular dichroism analysis gave 17% alpha helix, 33% beta sheet, and 21% beta turn for its secondary structure. Based on amino acid sequences of tryptic peptides, the protein was cloned and sequenced. The allergen consists of a single polypeptide chain of 102 aa, with a signal peptide of 21 residues. Ole e 10 showed homology with the C-terminal domain of another olive allergen, Ole e 9 (1,3-beta-glucanase, 53% identity), with deduced sequences from Arabidopsis thaliana genes (42-46% identity) and with polypeptide segments (Cys boxes) of proteins involved in yeast development (Epd1/Gas-1p/Phr2 families; 42-43% similarity). Ole e 10 showed 55% prevalence for olive-allergic patients and exhibited an IgE response dependent on its conformation. Remarkable IgE cross-reactivity was detected with Ole e 9, but no correlation was observed between the individual IgE responses to both allergens. Ole e 10 shares IgE B cell epitopes with proteins from Oleaceae, Gramineae, Betulaceae, Chenopodiaceae, Cupressaceae, Ambrosia, and Parietaria pollens, latex, and vegetable foods, such as tomato, kiwi, potato, and peach. These data indicate that Ole e 10 is a new pan-allergenic plant protein that shows notable intra- and interspecie IgE cross-reactivity and is a powerful candidate to be involved in pollen-latex-fruit syndrome.
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, Madrid, Spain.
Patricia
P
Barral
Eva
E
Batanero
Oscar
O
Palomares
Joaquín
J
Quiralte
Mayte
M
Villalba
Rosalía
R
Rodríguez
D000328:Adult; D000485:Allergens / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification* / Q000378:metabolism; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001483:Base Sequence; D001667:Binding, Competitive / Q000276:immunology; D003001:Cloning, Molecular; D003429:Cross Reactions; D018076:DNA, Complementary / Q000302:isolation & purification; D005638:Fruit / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007840:Latex / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000276:immunology; D031658:Olea / Q000235:genetics / Q000276:immunology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification* / Q000378:metabolism; D011058:Pollen / Q000235:genetics / Q000276:immunology*; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014675:Vegetables / Q000276:immunology / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
D000485:Allergens; D052179:Antigens, Plant; D018076:DNA, Complementary; D007840:Latex; C487628:Ole e 10 allergen, Olea europaea; D010940:Plant Proteins; D007073:Immunoglobulin E; C433244:OLE9 protein, Olea europaea; D001617:beta-Glucosidase