Individual protein balance strongly influences delta15N and delta13C values in Nile tilapia, Oreochromis niloticus.
Although stable isotope ratios in animals have often been used as indicators of the trophic level and for the back-calculation of diets, few experiments have been done under standardized laboratory conditions to investigate factors influencing delta(15)N and delta(13)C values. An experiment using Nile tilapia [ Oreochromis niloticus (L.)] was therefore carried out to test the effect of different dietary protein contents (35.4, 42.3, and 50.9%) on delta(15)N and delta(13)C values of the whole tilapia. The fish were fed the isoenergetic and isolipidic semi-synthetic diets at a relatively low level. delta(15)N and delta(13)C values of the lipid-free body did not differ between the fish fed the diets with different protein contents, but the trophic shift for N and C isotopes decreased with increasing protein accretion in the individual fish, for N from 6.5 per thousand to 4 per thousand and for C in the lipid-free body from 4 per thousand to 2.5 per thousand. This is the first study showing the strong influence of the individual protein balance to the degree to which the isotopic signature of dietary protein was modified in tissue protein of fish. The extrapolation of the trophic level or the reconstruction of the diet of an animal from stable isotope ratios without knowledge of the individual physiological condition and the feeding rate may lead to erroneous results.
Department of Aquaculture Systems and Animal Nutrition in the Tropics and Subtropics, University of Hohenheim (480b), Fruwirthstrasse 12, 70599 Stuttgart, Germany.
Julia
J
Gaye-Siessegger
Ulfert
U
Focken
Hansjörg
H
Abel
Klaus
K
Becker
D000821:Animal Feed; D000818:Animals; D001835:Body Weight; D002247:Carbon Isotopes / Q000378:metabolism*; D004044:Dietary Proteins; D004734:Energy Metabolism; D009587:Nitrogen Isotopes / Q000378:metabolism*; D017210:Tilapia / Q000502:physiology*; D015430:Weight Gain
D002247:Carbon Isotopes; D004044:Dietary Proteins; D009587:Nitrogen Isotopes