[The fibroblast primary cell culture by the split-thickness skin slide technique].
OBJECTIVE
To acquire lots of cell to culture during the primary cell culture.


METHODS
We take the split-thickness skin slide technique to acquire the dissociated fibroblast cell in two big-ear rats.


RESULTS
The cell number is above 10(6) from 1 cm x 2 cm split-thickness skin slide and the technique is simple, economic, effectve.


CONCLUSIONS
We think this way is better than other methods, and should be adopted in the primary cell culture, especially in fibroblast transplantation by injection.
Zhao
Yu-ming
YM
Department of the Aesthetic & Plastic Surgery on the Face and Neck, Plastic Surgery Hospital, Chinese Academy of Medical Science, Beijing 100041, China.
Zuo
Jin
J
Cao
Rui
R
Wang
Jia-qi
JQ
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D005347:Fibroblasts / Q000166:cytology* / Q000637:transplantation*; D011817:Rabbits