Crystallization and phasing of focal adhesion protein 52 from Gallus gallus.
Focal adhesion protein 52 (FAP52) is a multidomain adaptor protein of 448 amino acids characterized as an abundant component of focal adhesions. FAP52 binds to filamin via its N-terminal alpha-helical domain, suggesting a role in linking focal adhesions to the actin-based cytoskeleton. The recombinant protein was crystallized using the hanging-drop vapour-diffusion method, which yielded two crystal forms. Native data were collected from both crystal forms to 2.8 and 2.1 A resolution, respectively. For one of the crystal forms, initial MAD phasing was successfully performed using two data sets from xenon-derivatized crystals. The derivative data sets were collected using softer X-rays of 1.5 and 1.9 A wavelength. Preliminary structural analysis reveals the presence of a dimer in the asymmetric unit.
Töŕó
Imre
I
Structural Biology Laboratory, ELETTRA-Sincrotrone Trieste in Area Science Park, S.S. 14 Km 163, 5 loc. Basovizza, 34012 Trieste, Italy.
Nikki
Marko
M
Glumoff
Tuomo
T
Lehto
Veli-Pekka
VP
Djinović Carugo
Kristina
K
D000818:Animals; D002645:Chickens / Q000235:genetics / Q000378:metabolism; D003285:Contractile Proteins / Q000378:metabolism*; D003460:Crystallization; D018360:Crystallography, X-Ray; D064448:Filamins; D008840:Microfilament Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D003285:Contractile Proteins; D064448:Filamins; D008840:Microfilament Proteins; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; C108082:focal adhesion protein, 52-kDa