Acid-sensing ion channels ASIC2 and ASIC3 do not contribute to mechanically activated currents in mammalian sensory neurones.
The molecular basis of mechanosensory transduction by primary sensory neurones remains poorly understood. Amongst candidate transducer molecules are members of the acid-sensing ion channel (ASIC) family; nerve fibre recordings have shown ASIC2 and ASIC3 null mutants have aberrant responses to suprathreshold mechanical stimuli. Using the neuronal cell body as a model of the sensory terminal we investigated if ASIC2 or 3 contributed to mechanically activated currents in dorsal root ganglion (DRG) neurones. We cultured neurones from ASIC2 and ASIC3 null mutants and compared response properties with those of wild-type controls. Neuronal subpopulations [categorized by cell size, action potential duration and isolectin B4 (IB4) binding] generated distinct responses to mechanical stimulation consistent with their predicted in vivo phenotypes. In particular, there was a striking relationship between action potential duration and mechanosensitivity as has been observed in vivo. Putative low threshold mechanoreceptors exhibited rapidly adapting mechanically activated currents. Conversely, when nociceptors responded they displayed slowly or intermediately adapting currents that were smaller in amplitude than responses of low threshold mechanoreceptor neurones. No differences in current amplitude or kinetics were found between ASIC2 and/or ASIC3 null mutants and controls. Ruthenium red (5 microm) blocked mechanically activated currents in a voltage-dependent manner, with equal efficacy in wild-type and knockout animals. Analysis of proton-gated currents revealed that in wild-type and ASIC2/3 double knockout mice the majority of putative low threshold mechanoreceptors did not exhibit ASIC-like currents but exhibited a persistent current in response to low pH. Our findings are consistent with another ion channel type being important in DRG mechanotransduction.
Drew
Liam J
LJ
Molecular Nociception Group, Department of Biology, University College London, Gower Street, London WC1E 6BT, UK.
Rohrer
Daniel K
DK
Price
Margaret P
MP
Blaver
Karen E
KE
Cockayne
Debra A
DA
Cesare
Paolo
P
Wood
John N
JN
D062565:Acid Sensing Ion Channels; D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D001923:Brain Chemistry; D002211:Capsaicin / Q000494:pharmacology; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000737:chemistry / Q000187:drug effects / Q000502:physiology; D015870:Gene Expression / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D040542:Mechanotransduction, Cellular / Q000187:drug effects / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology*; D009475:Neurons, Afferent / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D009619:Nociceptors / Q000502:physiology; D018408:Patch-Clamp Techniques; D010812:Physical Stimulation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012430:Ruthenium Red / Q000494:pharmacology; D015222:Sodium Channels / Q000235:genetics / Q000502:physiology*; D013116:Spinal Cord / Q000737:chemistry; D013314:Stress, Mechanical; D013779:Tetrodotoxin / Q000494:pharmacology
C568265:ASIC2 protein, mouse; D062565:Acid Sensing Ion Channels; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D015222:Sodium Channels; D012430:Ruthenium Red; D013779:Tetrodotoxin; D002211:Capsaicin