FlyGEM, a full transcriptome array platform for the Drosophila community.
We have constructed a DNA microarray to monitor expression of predicted genes in Drosophila. By using homotypic hybridizations, we show that the array performs reproducibly, that dye effects are minimal, and that array results agree with systematic northern blotting. The array gene list has been extensively annotated and linked-out to other databases. Incyte and the NIH have made the platform available to the community via academic microarray facilities selected by an NIH committee.
Incyte Genomics, Palo Alto, CA 94304, USA.
Rick
R
Johnston
Bruce
B
Wang
Rachel
R
Nuttall
Michael
M
Doctolero
Pamela
P
Edwards
Jining
J
Lü
Marina
M
Vainer
Huibin
H
Yue
Xinhao
X
Wang
James
J
Minor
Cathy
C
Chan
Alex
A
Lash
Thomas
T
Goralski
Michael
M
Parisi
Brian
B
Oliver
Scott
S
Eastman
D000818:Animals; D015152:Blotting, Northern / Q000379:methods; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000138:chemical synthesis; D015342:DNA Probes / Q000096:biosynthesis; D018076:DNA, Complementary / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics*; D005091:Exons / Q000235:genetics; D005456:Fluorescent Dyes / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D017344:Genes, Insect / Q000235:genetics; D016678:Genome; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012313:RNA / Q000235:genetics; D015203:Reproducibility of Results
D017931:DNA Primers; D015342:DNA Probes; D018076:DNA, Complementary; D005456:Fluorescent Dyes; D012313:RNA; D004247:DNA