Temporal and topographic profiles of cyclooxygenase-2 expression during 24 h of focal brain ishemia in rats.
Substantial increases in cyclooxygenase-2 (COX-2) mRNA and protein levels were demonstrated in the peri-infarct and focal ischemic areas after 3-24 and 12-24 h, respectively, in rats. In the ischemic core, significant increases in COX-2 mRNA followed 6 h of ischemia, though the peak level was about one-third of that in the peri-infarct area. Increases in COX-2 protein in the ischemic core were not observed during ischemic periods. Diffuse, neuronal COX-2 staining was found in peri-infarct areas as well as in discrete, immunoreactive neurons in the ischemic core. Robust increases in prostaglandin E2 levels in the peri-infarct area were demonstrated following 24 h of ischemia. Prostaglandin production as well as COX-2 expression in ischemic tissues depended on the degree and duration of the reduction in cerebral blood flow.
Cerebrovascular Laboratory, Department of Pathogenesis, National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka, 565-8565, Japan. cyokota@ri.ncvc.go.jp
Chiaki
C
Yokota
Tomohito
T
Kaji
Yuji
Y
Kuge
Hiroyasu
H
Inoue
Nagara
N
Tamaki
Kazuo
K
Minematsu
D000704:Analysis of Variance; D000818:Animals; D001345:Autoradiography / Q000379:methods; D015152:Blotting, Northern / Q000379:methods; D001921:Brain / Q000098:blood supply / Q000378:metabolism*; D002545:Brain Ischemia / Q000201:enzymology* / Q000235:genetics; D002544:Cerebral Infarction / Q000473:pathology; D051546:Cyclooxygenase 2; D015232:Dinoprostone / Q000378:metabolism; D007839:Functional Laterality; D005786:Gene Expression Regulation; D007150:Immunohistochemistry / Q000379:methods; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow / Q000502:physiology; D013997:Time Factors
D007527:Isoenzymes; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone