Comparison of different probe-level analysis techniques for oligonucleotide microarrays.
Three different software packages for the probe-level analysis of high-density oligonucleotide microarray data were compared using an experiment-derived data set that was validated using real-time PCR. The efficiency with which these three programs could identify true positives in this data set was assessed. In addition, estimates of false-positive and false-negative rates were determined. The performance of the programs using very small data sets was also compared, and recommendations for use are suggested.
State University of New York at Stony Brook, Stony Brook, NY, USA.
Barbara
B
Rosati
Frederic
F
Grau
Anneke
A
Kuehler
Samantha
S
Rodriguez
David
D
McKinnon
D000818:Animals; D005188:False Negative Reactions; D005189:False Positive Reactions; D020869:Gene Expression Profiling / Q000379:methods; D009206:Myocardium / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats
D015345:Oligonucleotide Probes