Intratracheal albumin reduces interleukin-8 in tracheobronchial aspirates in piglets after meconium aspiration.
Meconium aspiration induces pulmonary inflammation and reduces surfactant function. We hypothesized that albumin mixed with meconium attenuates pulmonary inflammation and improves surfactant function after meconium aspiration. We measured the concentration of free fatty acids (FFA) in the meconium (110 mg dry weight/mL) and added albumin to provide a molar FFA:albumin ratio of 1:1. Newborn piglets, 0-2 day of age, artificially ventilated and exposed to hypoxemia by ventilation with 8% O2, were randomized to group A receiving meconium (n = 12), or group B receiving meconium + albumin (n = 12), 3 ml/kg intratracheally. The animals were reoxygenated for 8 h. Reoxygenation was started when mean arterial blood pressure was < 20 mm Hg or base excess was < -20 mmol/L. During 8 h of reoxygenation the interleukin-8 concentrations in tracheobronchial aspirates increased 5-fold more in the meconium vs. the meconium + albumin groups (93 +/- 56 vs. 18 +/- 4 pg/mL, p < 0.005). There were no differences between the groups for tumor necrosis factor alpha in tracheobronchial aspirates, recruitment of inflammatory cells in the airspaces or surfactant function in bronchoalveolar lavage fluid. In conclusion, albumin significantly decreased interleukin-8 concentrations in tracheobronchial aspirates after meconium aspiration.
Department of Pediatric Research, the National Hospital, Oslo, Norway. p.a.tollofsrud@klinmed.uio.no
Per Arne
PA
Tølløfsrud
Sverre
S
Medbø
Anne Beate
AB
Solås
Bengt
B
Robertson
Christian P
CP
Speer
Silvia
S
Seidenspinner
Christian A
CA
Drevon
Ola Didrik
OD
Saugstad
D000818:Animals; D000831:Animals, Newborn; D001980:Bronchi / Q000378:metabolism*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D006801:Humans; D000860:Hypoxia; D007231:Infant, Newborn; D016209:Interleukin-8 / Q000032:analysis*; D008168:Lung / Q000473:pathology; D008470:Meconium / Q000737:chemistry / Q000502:physiology; D008471:Meconium Aspiration Syndrome / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D010100:Oxygen / Q000008:administration & dosage; D011663:Pulmonary Surfactants / Q000032:analysis; D012710:Serum Albumin, Bovine / Q000008:administration & dosage*; D013500:Surface Tension; D013552:Swine; D014132:Trachea / Q000187:drug effects / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D005230:Fatty Acids, Nonesterified; D016209:Interleukin-8; D011663:Pulmonary Surfactants; D014409:Tumor Necrosis Factor-alpha; D012710:Serum Albumin, Bovine; D010100:Oxygen