Oncodevelopmental alpha-fetoprotein acts as a selective proangiogenic factor on endothelial cell from the fetomaternal unit.
The molecular coordination between angiogenesis and vascular remodeling is a critical step for the development of a functional vasculature in the placenta and the uterus during pregnancy. The oncodevelopmental albumin homolog alpha-fetoprotein (AFP) is mainly synthesized in the developing fetus, and its expression has been found to be associated with highly vascularized tumors in the adult. In this study, we investigated the angiogenic activity of AFP and its possible role in the fetomaternal unit. Immunohistochemical studies revealed that the AFP-binding protein(s) is expressed in blood vessels of chorionic villi from placentae of the second and the third but not of the first trimester during pregnancy. At low concentrations, AFP directly stimulates or enhances, respectively, vascular endothelial growth factor-induced proliferation and sprout formation of endothelial cells isolated from the placenta and the uterus possibly by a MAPK-dependent pathway. Furthermore, AFP enhances blood vessel formation in a chick chorioallantoic membrane assay in vivo. Interestingly, AFP has no proliferative or migratory effects on endothelial cells isolated from the umbilical vein in the absence of vascular endothelial growth factor. These data indicate that AFP may act as a specific proangiogenic factor of endothelial cells within the fetomaternal unit during advanced stages in pregnancy.
Department of Obstetrics and Gynecology, Justus-Liebig-University, D-35385 Giessen, Germany.
Olin D
OD
Liang
Thomas
T
Korff
Jessica
J
Eckhardt
Jasmin
J
Rifaat
Nelli
N
Baal
Friederike
F
Herr
Klaus T
KT
Preissner
Marek
M
Zygmunt
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002642:Chick Embryo; D002824:Chorionic Villi / Q000098:blood supply / Q000502:physiology; D004357:Drug Synergism; D004727:Endothelium / Q000098:blood supply* / Q000166:cytology / Q000502:physiology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008863:Microspheres; D018919:Neovascularization, Physiologic / Q000502:physiology*; D011247:Pregnancy; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology; D000509:alpha-Fetoproteins / Q000494:pharmacology / Q000502:physiology*
D042461:Vascular Endothelial Growth Factor A; D000509:alpha-Fetoproteins