FlyGEM, a full transcriptome array platform for the Drosophila community.
We have constructed a DNA microarray to monitor expression of predicted genes in Drosophila. By using homotypic hybridizations, we show that the array performs reproducibly, that dye effects are minimal, and that array results agree with systematic northern blotting. The array gene list has been extensively annotated and linked-out to other databases. Incyte and the NIH have made the platform available to the community via academic microarray facilities selected by an NIH committee.
Johnston
Rick
R
Incyte Genomics, Palo Alto, CA 94304, USA.
Wang
Bruce
B
Nuttall
Rachel
R
Doctolero
Michael
M
Edwards
Pamela
P
Lü
Jining
J
Vainer
Marina
M
Yue
Huibin
H
Wang
Xinhao
X
Minor
James
J
Chan
Cathy
C
Lash
Alex
A
Goralski
Thomas
T
Parisi
Michael
M
Oliver
Brian
B
Eastman
Scott
S
D000818:Animals; D015152:Blotting, Northern / Q000379:methods; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000138:chemical synthesis; D015342:DNA Probes / Q000096:biosynthesis; D018076:DNA, Complementary / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics*; D005091:Exons / Q000235:genetics; D005456:Fluorescent Dyes / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D017344:Genes, Insect / Q000235:genetics; D016678:Genome; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012313:RNA / Q000235:genetics; D015203:Reproducibility of Results
D017931:DNA Primers; D015342:DNA Probes; D018076:DNA, Complementary; D005456:Fluorescent Dyes; D012313:RNA; D004247:DNA