Activation of activator protein-1 in cells exposed to electrical stimulation of various parameters used in electroacupuncture therapy.
The aim of this study was to investigate the effect of electrical stimulation on activator protein-1 (AP-1) in recombinant liver cells. In order to elucidate the molecular effects of electrical stimulation on cells, AP-1 expression was detected by a luciferase assay. The parameters used were taken from clinical electroacupuncture (EA) therapy as follows: biphasic rectangular symmetrical pulses (frequency: 2, 10 and 100 Hz; pulse width: 50, 80, 130 and 250 microsec; intensity: 1, 2, 5 and 10 mA; time: 5, 10, 20 and 30 minutes). S (10% fetal bovine serum in medium), SF (serum free medium) and 12-O-tetradecanoylphorbol-13-acetate (TPA) treatment represented the experimental, negative and positive groups, respectively. We found that electrical stimulation with 10 Hz, a pulse width of 130 microsec, and a duration of 30 minutes gave a significant increase in AP-1 activity. In contrast, the intensity of the stimulation had no significant effect on AP-1 activity. In conclusion, our data suggest that electrical stimulation with an appropriate frequency, duration and pulse width could cause an increase in AP-1 activity in cells.
Hsu
Sheng-Feng
SF
Acupuncture Department, China Medical University Hospital, School of Chinese Medicine, China Medical University Taichung, Taiwan, ROC.
Lin
Jaung-Geng
JG
Liu
Hsu-Jan
HJ
Lai
I Lu
IL
Wu
Shih-Lu
SL
Hsiangt
Chien-Yun
CY
Hot
Tin-Yun
TY
D002478:Cells, Cultured / Q000502:physiology; D004558:Electric Stimulation; D015671:Electroacupuncture; D015870:Gene Expression; D017930:Genes, Reporter; D006801:Humans; D008099:Liver / Q000166:cytology*; D008156:Luciferases / Q000235:genetics; D018808:Transcription Factor AP-1 / Q000235:genetics*; D014162:Transfection
D018808:Transcription Factor AP-1; D008156:Luciferases