Emmprin promotes anchorage-independent growth in human mammary carcinoma cells by stimulating hyaluronan production.
Emmprin (CD147; basigin) is a plasma membrane glycoprotein, enriched on the surface of many cancer cells, which induces matrix metalloproteinase synthesis via cell-cell interactions. Elevated emmprin production causes increased growth in vivo of human mammary carcinoma cells. In this study, we show that elevation of emmprin expression in less aggressive human carcinoma cells, which normally express low emmprin levels, induces the ability to grow under anchorage-independent conditions. We also found that elevated emmprin expression stimulates hyaluronan production and that the effect of emmprin on anchorage-independent growth is dependent on hyaluronan. Furthermore, emmprin stimulates cell survival pathway signaling in a hyaluronan-dependent manner. From these and other studies we conclude that emmprin enhances several malignant properties of cancer cells, including anchorage-independent growth, invasiveness, and chemoresistance.
Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, MA, USA.
Erica A
EA
Marieb
Alexandra
A
Zoltan-Jones
Rongsong
R
Li
Suniti
S
Misra
Shibnath
S
Ghatak
Jian
J
Cao
Stanley
S
Zucker
Bryan P
BP
Toole
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D051926:Basigin; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D002455:Cell Division; D045744:Cell Line, Tumor; D002470:Cell Survival; D005260:Female; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D008562:Membrane Glycoproteins / Q000502:physiology*
D015703:Antigens, CD; D000951:Antigens, Neoplasm; C497886:BSG protein, human; D008562:Membrane Glycoproteins; D051926:Basigin; D006820:Hyaluronic Acid