[Determination of the inhibitory activity of angiotensin-converting enzyme inhibitor captopril by high performance capillary electrophoresis].
OBJECTIVE
To establish a method for determinate of the inhibitory activity of angiotensin-converting enzyme inhibitor captopril by high performance capillary electrophoresis.


METHODS
The characteristic absorptive wavelength of hippuric acid determined by ultraviolet spectrophotometer is 228 nm. The method employed a melted capillary column, 50 mmol.L-1 phosphoric acid (pH 8.3) buffer solution, inject pressure 4.8 kPa, inject time 3 s, separation voltage 20 kV and detection wavelength 228 nm.


RESULTS
The reactant and resultant was separated completed within 7 min. IC50 of captopril was 0.019 mumol.L-1. Captopril is a competitive inhibitor, which was proved by enzyme reaction dynamics.


CONCLUSIONS
The method was shown to be accurate, simple and rapid and can be used for determination of the inhibitory activity of captopril.
Xin
Zhi-hong
ZH
College of Biological and Engineering, Jiangsu University, Zhenjiang 212013, China.
Ma
Hai-le
HL
Wu
Shou-yi
SY
Dai
Chun-hua
CH
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D002216:Captopril / Q000494:pharmacology*; D019075:Electrophoresis, Capillary / Q000379:methods; D006626:Hippurates / Q000032:analysis*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*
D000806:Angiotensin-Converting Enzyme Inhibitors; D006626:Hippurates; D002216:Captopril; D007703:Peptidyl-Dipeptidase A; C030514:hippuric acid