Water chloride provides partial protection during chronic exposure to waterborne silver in rainbow trout (Oncorhynchus mykiss) embryos and larvae.
Rainbow trout embryos and larvae were continuously exposed (at 12.5 degrees C) to waterborne silver in a flow-through setup, from fertilization to swim-up, at nominal silver concentrations of 0, 0.1, or 1.0 microg/L total silver (as AgNO(3)) at three different water Cl(-) levels (30, 300, and 3,000 microM, added as KCl). Exposures were conducted in synthetic soft water (hardness 20 mg CaCO(3)/L generated from reconstituted reverse osmosis freshwater). Continuous exposure to 1.0 microg/L total silver for 58 d at 30 microM water Cl(-) resulted in a pronounced ionoregulatory disturbance (as indicated by a reduction in whole body Na(+),K(+)-ATPase activity, unidirectional Na(+) uptake [Jin Na(+)], and whole body Na(+) and Cl(-) levels) and a reduction in extractable protein and wet weight. Thus, the mechanism of chronic silver toxicity appears to be similar to that observed during acute silver exposure in juvenile and adult fish, specifically an ionoregulatory disturbance. Higher water Cl(-) levels (300 and 3,000 microM Cl(-)) offered some degree of protection from the ionoregulatory disturbance, with only minor protective effects in terms of mortality. The protective effects of water Cl(-) on the toxicity of silver (as AgNO(3)) appear to be far less during chronic than during acute exposure. Mortality and larval Na(+) concentration, Jin Na(+), and Na(+),K(+)-ATPase activity all appear to be correlated with silver body burden and calculated water Ag(+) during chronic silver exposure. Thus, there appears to be potential to model chronic toxicity but not simply by recalibration of an acute model. A chronic model must be based on real chronic data because the protective effects of various ligands appear to be quantitatively very different from those in the acute situation.
Department of Zoology, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada. brauner@zoology.ubc.ca
Colin J
CJ
Brauner
Jonathan
J
Wilson
Collins
C
Kamunde
Chris M
CM
Wood
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D027682:Cation Transport Proteins; D002712:Chlorides / Q000378:metabolism*; D003124:Colorimetry; D004625:Embryo, Nonmammalian / Q000502:physiology; D005618:Fresh Water / Q000032:analysis; D007814:Larva / Q000502:physiology; D008956:Models, Chemical; D017686:Oncorhynchus mykiss / Q000196:embryology / Q000378:metabolism / Q000502:physiology*; D011188:Potassium / Q000378:metabolism; D012834:Silver / Q000378:metabolism / Q000493:pharmacokinetics / Q000633:toxicity*; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase; D014882:Water-Electrolyte Balance / Q000187:drug effects* / Q000502:physiology
D027682:Cation Transport Proteins; D002712:Chlorides; D012834:Silver; D012964:Sodium; D000251:Adenosine Triphosphatases; C022761:potassium transporting ATPase; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium