Protective effects of B2 preservation solution in comparison to a standard solution (histidine-tryptophan-ketoglutarate/Bretschneider) in a model of isolated autologous hemoperfused porcine kidney.
Reperfusion injuries after organ transplantation affect graft function and influence long-term graft survival. As hypothermic storage, which minimizes the extent of unspecific tissue injury after ischemia and reperfusion, is significantly influenced by the composition of preservation solutions, strategies to optimize the different components may lead to longer graft survival. In the present study the effects of the preservation solution B2 on early renal function and histopathological changes were compared to histidine-tryptophan-ketoglutarate solution (HTK, Bretschneider) in a model of isolated blood-perfused porcine kidneys. B2-preserved kidneys displayed a lower renal resistance and significantly better creatinine clearance as compared to HTK. Mean differences were also found for filtration fraction and sodium fraction reabsorption. The functional data were also related to histopathological changes. Together, these data indicate that the recently developed preservation solution B2 offers new principles of preservation and is a useful preservation solution for experimental isolated perfused kidney models. B2 may also be an interesting model for optimizing preservation within other organ perfusion models.
Fehrenberg
Claudia
C
Department of Comparative Medicine and Experimental Animal Science, Humboldt University, Berlin, Germany. claudia.fehrenberg@charite.de
von Baeyer
Hans
H
Unger
Volker
V
Schmitt
Roland
R
Haider
Wolfram
W
Quarcoo
David
D
Groneberg
David A
DA
Grosse-Siestrup
Christian
C
D000818:Animals; D005947:Glucose / Q000737:chemistry; D007668:Kidney / Q000473:pathology / Q000502:physiology; D008353:Mannitol / Q000737:chemistry; D009924:Organ Culture Techniques; D019188:Organ Preservation Solutions / Q000737:chemistry; D010477:Perfusion; D011189:Potassium Chloride / Q000737:chemistry; D011343:Procaine / Q000737:chemistry; D013552:Swine
C485295:B2 preservation solution; C039984:Bretschneider cardioplegic solution; D019188:Organ Preservation Solutions; D008353:Mannitol; D011343:Procaine; D011189:Potassium Chloride; D005947:Glucose