Absence of mutations in the Wilms' tumor gene wt1 in de novo non-small cell lung cancers.
We recently demonstrated that the WT1 gene was overexpressed in the majority of de novo lung cancers regardless of cancer subtypes. Here, we examined WT1 genomic DNA in 38 cases of de novo non-small cell lung cancers (NSCLC) for mutations using direct sequencing. The sequencing analysis showed no mutations of WT1 genomic DNA in any of 38 de novo non-small cell lung cancers examined. These results indicated that the non-mutated, wild-type WT1 gene played an important role in de novo NSCLC.
Department of Functional Diagnostic Science, Osaka University Graduate School of Medicine, Japan.
Y
Y
Oji
S
S
Miyoshi
E
E
Takahashi
S
S
Koga
Y
Y
Nakano
Y
Y
Shintani
H
H
Hirabayashi
A
A
Matsumura
K
K
Iuchi
K
K
Ito
Y
Y
Kishimoto
A
A
Tsuboi
K
K
Ikegame
N
N
Hosen
Y
Y
Oka
H
H
Ogawa
H
H
Maeda
S
S
Hayashi
I
I
Kawase
H
H
Sugiyama
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D005260:Female; D016162:Genes, Wilms Tumor; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D020641:Polymorphism, Single Nucleotide