A method for the direct evaluation of the fatty acid status in a drop of blood from a fingertip in humans: applicability to nutritional and epidemiological studies.
Several studies have shown that the fatty acid composition of circulating lipids reflects dietary fat intake, in turn being related to health status. The fatty acid composition of plasma lipids is therefore an important parameter in studies on dietary interventions. The aim of our study was to develop a rapid and inexpensive method for the analysis of circulating fatty acids applicable to large population groups. Drops of blood collected from fingertips have been directly subjected to transmethylation for gas chromatography analysis. This new method, validated for reproducibility, has been compared with the conventional method, based on withdrawal of blood from the antecubital vein followed by lipid extraction, and identical data have been obtained with the two techniques. Observed and predicted differences between blood and plasma fatty acids are related to the contribution of circulating cell membranes in blood. Finally the application of the methods to samples from 100 healthy subjects and the assessed correlation between dietary habits and blood fatty acid profiles demonstrate the validity of the new method and its applicability to nutritional and epidemiological studies.
Department of Pharmacological Sciences, University of Milan, 20133 Milan, Italy.
Franca
F
Marangoni
Claudio
C
Colombo
Claudio
C
Galli
D000328:Adult; D000368:Aged; D002849:Chromatography, Gas / Q000379:methods*; D004812:Epidemiologic Methods; D005227:Fatty Acids / Q000097:blood*; D005240:Feasibility Studies; D005260:Female; D005385:Fingers / Q000098:blood supply*; D006801:Humans; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D015596:Nutrition Assessment; D012016:Reference Values; D015203:Reproducibility of Results; D018401:Sample Size
D005227:Fatty Acids; D008055:Lipids