Cloning and sequencing of a 16 kDa outer membrane protein gene of Pasteurella multocida P52.
The outer membrane proteins (OMPs) of Pasteurella multocida are potential immunogens. The 16 kDa OMP of P. multocida P52, serotype B:2, was identified as one of the major immunodominant antigens. The gene omp16, encoding a 16 kDa outer membrane protein, was amplified, cloned into a pBluescript SK(-) vector and sequenced. Complete sequence homology was observed between the 16 kDa OMP gene of P. multocida P52 (serotypes B:2) and P. multocida T16 (somatic serotype 3,4). This gene was distributed among different serotypes of P. multocida and found to localize in a 6.0 kb HindII fragment of the P. multocida genome.
National Biotechnology Centre, Division of Pathology, Indian Veterinary Research Institute, Izatnagar 243 12, U.P., India. ppgnbc@ivri.up.nic.in
P P
PP
Goswami
P
P
Chaudhuri
V
V
Tiwari
N S
NS
Parihar
P C
PC
Harbola
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D001428:Bacterial Vaccines; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D015151:Immunoblotting; D008969:Molecular Sequence Data; D010326:Pasteurella Infections / Q000517:prevention & control*; D016979:Pasteurella multocida / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D004269:DNA, Bacterial