Roles of histone acetylation and chromatin remodeling factor in a meiotic recombination hotspot.
Histone acetyltransferases (HATs) and ATP-dependent chromatin remodeling factors (ADCRs) are involved in selective gene regulation via modulation of local chromatin configuration. Activation of the recombination hotspot ade6-M26 of Schizosaccharomyces pombe is mediated by a cAMP responsive element (CRE)-like sequence, M26, and a heterodimeric ATF/CREB transcription factor, Atf1.Pcr1. Chromatin remodeling occurs meiotically around M26. We examined the roles of HATs and ADCRs in chromatin remodeling around M26. Histones H3 and H4 around M26 were hyperacetylated in an M26- and Atf1-dependent manner early in meiosis. SpGcn5, the S. pombe homolog of Gcn5p, was required for the majority of histone H3 acetylation around M26 in vivo. Deletion of gcn5+ caused a significant delay in chromatin remodeling but only partial reduction of M26 meiotic recombination frequency. The snf22+ (a Swi2/Snf2-ADCR homologue) deletion and snf22+ gcn5+ double deletion abolished chromatin remodeling and significant reduction of meiotic recombination around M26. These results suggest that HATs and ADCRs cooperatively alter local chromatin structure, as in selective transcription activation, to activate meiotic recombination at M26 in a site-specific manner.
Genetic Dynamics Research Unit-Laboratory, RIKEN, Wako, Saitama, Japan.
Takatomi
T
Yamada
Ken-ichi
K
Mizuno
Kouji
K
Hirota
Ning
N
Kon
Wayne P
WP
Wahls
Edgar
E
Hartsuiker
Hiromu
H
Murofushi
Takehiko
T
Shibata
Kunihiro
K
Ohta
D000107:Acetylation; D000123:Acetyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D051697:Activating Transcription Factor 1; D051696:Activating Transcription Factors; D000595:Amino Acid Sequence; D042002:Chromatin Assembly and Disassembly; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D005800:Genes, Fungal / Q000235:genetics; D006657:Histones / Q000378:metabolism*; D008540:Meiosis / Q000235:genetics*; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic / Q000235:genetics*; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000099224:p300-CBP-Associated Factor
D000123:Acetyltransferases; D051697:Activating Transcription Factor 1; D051696:Activating Transcription Factors; D004268:DNA-Binding Proteins; D006657:Histones; D010750:Phosphoproteins; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; C097701:Pcr1 protein, S pombe; C106740:atf1 protein, S pombe; C501323:snf22 protein, S pombe; C501322:Gcn5 protein, S pombe; D000099224:p300-CBP-Associated Factor