Assessment of novel diazinon immunoassays for water analysis.
Diazinon is a broad organophosphate insecticide used in agricultural and other treatments, resulting in widespread water contamination. The development of easy-to-use screening immunoanalytical methods is an interesting tool to study environmental pollution impact. Two novel strategies for diazinon hapten synthesis are addressed. One of them attaches the spacer arm to the oxygen atom of the diazinon aromatic ring. The other one retains the diazinon basic structure linking the spacer to an aromatic carbon. A total of eight diazinon haptens were synthesized, demonstrating that they are suitable for immunoreagent (protein conjugates and polyclonal antibodies) production. The optimized ELISA is based on conjugate-coated format and had a detection limit of 0.40 microg/L, showing little or no cross-reactivity to similar tested compounds. The immunoassays were used as a tool to quantify diazinon in natural waters. Results are in agreement with those given by GC-MS reference method. Mean recoveries ranging between 99% and 105% confirm the potential of our approach to determine diazinon in samples without purification or preconcentration steps, being applied as a screening method for field monitoring of diazinon in river waters.
Departamento de Química, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022 Valencia, Spain.
Eva M
EM
Brun
Marta
M
Garcés-García
Estefanía
E
Escuín
Sergi
S
Morais
Rosa
R
Puchades
Angel
A
Maquieira
D000818:Animals; D000917:Antibody Formation; D003976:Diazinon / Q000032:analysis* / Q000276:immunology; D004784:Environmental Monitoring / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007306:Insecticides / Q000032:analysis* / Q000276:immunology; D011817:Rabbits; D045483:Rivers; D012680:Sensitivity and Specificity; D014874:Water Pollutants, Chemical / Q000032:analysis*
D007306:Insecticides; D014874:Water Pollutants, Chemical; D003976:Diazinon