Metalated hybrid polymers as catalytic reagents for phosphate ester hydrolysis and plasmid modification.
Pendant pyrazolylcyclophosphazene containing hybrid cross-linked polymer (CPPL) has been utilized for binding Zn(II). The metalated polymer (CPPL-Zn) has been found to be very effective catalyst for the hydrolysis of a RNA model phosphodiester substrate [2-(hydroxypropyl)-p-nitrophenyl phosphate, hNPP]. In addition, CPPL-Zn also cleaved supercoiled plasmid DNA pBR322 thus providing a novel structural motif of inorganic-organic hybrid polymers as synthetic nucleases.
Department of Chemistry, Indian Institute of Technology, Kanpur-208016, India. vc@iitk.ac.in
Vadapalli
V
Chandrasekhar
Pravas
P
Deria
Venkatasubbaiah
V
Krishnan
Arunachalampillai
A
Athimoolam
Sanjay
S
Singh
C
C
Madhavaiah
S G
SG
Srivatsan
Sandeep
S
Verma
D002384:Catalysis / Q000187:drug effects; D004952:Esters; D006868:Hydrolysis / Q000187:drug effects; D008670:Metals / Q000737:chemistry / Q000493:pharmacokinetics*; D010710:Phosphates / Q000737:chemistry / Q000493:pharmacokinetics*; D010957:Plasmids / Q000737:chemistry / Q000493:pharmacokinetics*; D011108:Polymers / Q000737:chemistry / Q000493:pharmacokinetics*
D004952:Esters; D008670:Metals; D010710:Phosphates; D011108:Polymers