Human herpesvirus 6 infection arrests cord blood mononuclear cells in G(2) phase of the cell cycle.
We here report that after infection with human herpesvirus 6A, human cord blood mononuclear cells accumulate in G(2)/M phase of the cell cycle. Experiments with foscarnet or ultraviolet (UV)-irradiated virus stocks pointed at an (immediate-)early, newly formed viral protein to be responsible for the arrest. At the molecular level, p53, cyclin B(1), cyclin A and tyrosine(15)-phosphorylated cdk1 accumulated after HHV-6A infection, indicating an arrest in G(2). However, no change was observed in the levels of downstream effectors of p53 in establishing a G(2) arrest, i.e. p21 and 14-3-3sigma. We thus conclude that the HHV-6A-induced G(2) arrest occurs independently of p53 accumulation.
De Bolle
Leen
L
Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium. leen.debolle@rega.kuleuven.ac.be
Hatse
Sigrid
S
Verbeken
Erik
E
De Clercq
Erik
E
Naesens
Lieve
L
D000998:Antiviral Agents / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D019925:Cyclin A / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D019926:Cyclin B / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D018844:Cyclin-Dependent Kinases / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D004247:DNA / Q000032:analysis; D005312:Fetal Blood / Q000166:cytology*; D017245:Foscarnet / Q000494:pharmacology; D016195:G2 Phase / Q000187:drug effects / Q000528:radiation effects; D015654:Herpesvirus 6, Human / Q000502:physiology*; D006801:Humans; D009000:Monocytes / Q000187:drug effects / Q000528:radiation effects; D019349:Roseolovirus Infections / Q000097:blood; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D014466:Ultraviolet Rays
D000998:Antiviral Agents; D019925:Cyclin A; D019926:Cyclin B; D016159:Tumor Suppressor Protein p53; D017245:Foscarnet; D004247:DNA; D018844:Cyclin-Dependent Kinases