Stability of polymorphic GC-rich repeat sequence-containing regions of Mycobacterium tuberculosis.
Mycobacterium tuberculosis cultures were subjected to DNA fingerprinting with IS6110- and polymorphic GC-rich sequence (PGRS)-containing probes. The PGRS banding patterns remained highly stable during multiple cultures of specimens from one disease episode (0.5% changed) and during transmission in patients with close contact (1.9% changed). Characteristic PGRS-restriction fragment length polymorphism motifs for different strain groupings may indicate distant evolutionary events leading to the differentiation of M. tuberculosis strain lineages.
Richardson
Madalene
M
MRC Centre for Molecular and Cellular Biology, Department of Medical Biochemistry, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa. mr6@sun.ac.za
van der Spuy
Gian D
GD
Sampson
Samantha L
SL
Beyers
Nulda
N
van Helden
Paul D
PD
Warren
Robin M
RM
D003596:Cytosine; D016172:DNA Fingerprinting; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D018900:Dinucleotide Repeats / Q000235:genetics*; D019143:Evolution, Molecular; D016680:Genome, Bacterial; D006147:Guanine; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D014376:Tuberculosis
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D006147:Guanine; D003596:Cytosine