Cyanide as a copper and quinone-directed inhibitor of amine oxidases from pea seedlings ( Pisum sativum) and Arthrobacter globiformis: evidence for both copper coordination and cyanohydrin derivatization of the quinone cofactor.
The interactions of cyanide with two copper-containing amine oxidases (CuAOs) from pea seedlings (PSAO) and the soil bacterium Arthrobacter globiformis (AGAO) have been investigated by spectroscopic and kinetic techniques. Previously, we rationalized the effects of azide and cyanide for several CuAOs in terms of copper coordination by these exogenous ligands and their effects on the internal redox equilibrium TPQ(amr)-Cu(II) right harpoon over left harpoon TPQ(sq)-Cu(I). The mechanism of cyanide inhibition was proposed to occur through complexation to Cu(I), thereby directly competing with O(2) for reoxidation of TPQ. Although cyanide readily and reversibly reacts with quinones, no direct spectroscopic evidence for cyanohydrin derivatization of TPQ has been previously documented for CuAOs. This work describes the first direct spectroscopic evidence, using both model and enzyme systems, for cyanohydrin derivatization of TPQ. K(d) values for Cu(II)-CN(-) and Cu(I)-CN(-), as well as the K(i) for cyanide inhibition versus substrate amine, are reported for PSAO and AGAO. In spite of cyanohydrin derivatization of the TPQ cofactor in these enzymes, the uncompetitive inhibition of amine oxidation is determined to arise almost exclusively through CN(-) complexation of Cu(I).
Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, USA.
Eric M
EM
Shepard
Gregory A
GA
Juda
Ke-Qing
KQ
Ling
Lawrence M
LM
Sayre
David M
DM
Dooley
D006631:Amine Oxidase (Copper-Containing) / Q000037:antagonists & inhibitors* / Q000378:metabolism; D001173:Arthrobacter / Q000201:enzymology* / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D016227:Benzoquinones / Q000378:metabolism / Q000494:pharmacology; D003067:Coenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism; D003300:Copper / Q000378:metabolism / Q000494:pharmacology; D003486:Cyanides / Q000378:metabolism* / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000378:metabolism / Q000494:pharmacology; D009570:Nitriles / Q000378:metabolism*; D018532:Pisum sativum / Q000201:enzymology*; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D036226:Seedlings / Q000201:enzymology
D001426:Bacterial Proteins; D016227:Benzoquinones; D003067:Coenzymes; D003486:Cyanides; D004791:Enzyme Inhibitors; D009570:Nitriles; D010940:Plant Proteins; C430593:cyanohydrin; C004532:quinone; D003300:Copper; D006631:Amine Oxidase (Copper-Containing)