Biosynthesis and characterization of 14C-enriched flavonoid fractions from plant cell suspension cultures.
A range of radiolabeled anthocyanins, proanthocyanidins, and other flavonoids were accumulated by cell suspension cultures of two plant species, ohelo (Vaccinium pahalae) and grape (a Vitis hybrid, Bailey Alicant A), after providing uniformly labeled [(14)C]sucrose to the medium. Approximately 15% of administered label was recovered in a series of flavonoid-rich fractions varying in composition. Anthocyanins, and monomers to oligomers of proanthocyanidins, were labeled effectively and characterized from both species. Most of the proanthocyanidin oligomers were based on the flavan-3-ols (+)-catechin and (-)-epicatechin. Cyanidin and peonidin glycosides were the dominant forms of anthocyanins in both species. Whereas the predominant form of flavonoids identified from ohelo cell cultures was proanthocyanidins, grape cell cultures produced mostly anthocyanins. The labeled phytochemicals were produced for use in subsequent in vivo animal feeding studies to gauge their bioavailability and accumulation in target organs.
Yousef
Gad G
GG
Department of Natural Resources and Environmental Sciences, University of Illinois at Urbana-Champaign, 1201 South Dorner Drive, Urbana, Illinois 61801, USA.
Seigler
David S
DS
Grusak
Michael A
MA
Rogers
Randy B
RB
Knight
Christopher T G
CT
Kraft
Tristan F B
TF
Erdman
John W
JW
Lila
Mary Ann
MA
D000872:Anthocyanins / Q000032:analysis / Q000096:biosynthesis; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005419:Flavonoids / Q000032:analysis / Q000096:biosynthesis*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D029796:Vaccinium / Q000378:metabolism*; D027843:Vitis / Q000378:metabolism*
D000872:Anthocyanins; D005419:Flavonoids