Ambiguities of human leukocyte antigen-B resolved by sequence-based typing of exons 1, 4, and 5.
The elucidation of the sequences of human leukocyte antigen-B (HLA-B)-exons 1 through 5 has led to an increase of ambiguities with alleles having identical exon 2 and 3 sequences, but differences in other exons. At the moment, 26 HLA-B alleles show such ambiguities which can be resolved by sequencing the exons in which the differences are located. Here we report a sequence-based typing (SBT) strategy for heterozygous sequencing of exons 1, 4, and 5, in addition to the previously described exons 2 and 3. The strategy was validated against a panel of 25 individuals, carrying HLA-B alleles from 33 different allele groups. Correct assignment of all HLA-B alleles was obtained for exons 1 through 5. In addition, the SBT protocol was used to resolve ambiguities in 50 individuals. The ambiguous combinations studied were B*0705/06, B*0801/19N, B*1512/19, B*180101/17N, B*270502/13/0504, B*350101/42/40N, B*390101/0103, B*400102/0101, B*440201/19N/27, and B*510101/11N/0105/30/32. In all cases, sequencing revealed the first allele to be present, except for three individuals with B*07. One of them typed B*0705; the other two were B*0706. The described SBT protocol for sequencing exons 1, 4, and 5 is a valuable tool for resolving ambiguities of HLA-B alleles with differences in these exons, as well as for studying the polymorphism of HLA-B outside exons 2 and 3.
Tissue Typing Laboratory, University Hospital Maastricht, the Netherlands. evdbl@lwee.azm.nl
W T N
WT
Swelsen
C E M
CE
Voorter
E M
EM
van den Berg-Loonen
D001483:Base Sequence; D015343:DNA Probes, HLA / Q000235:genetics; D005091:Exons; D005260:Female; D015235:HLA-B Antigens / Q000235:genetics*; D006801:Humans; D007962:Leukocytes / Q000276:immunology; D008297:Male; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA
D015343:DNA Probes, HLA; D015235:HLA-B Antigens