[Characteristic analysis of acute leukemia immunophenotyping in 180 cases by flow cytometry].
To study the acute Leukemia immunophenotype and its diagnosis value, three color direct immunofluorescece staining methods of flow cytometry and immunophenotype of antibody integration system were used for detection of 180 cases of acute leukemia. The results showed that patients with ALL expressed lymphocyte antigen, and 49.4% patients with ALL accompanied myeloid antigen; all patients with AML expressed myeloid antigen, and 43.2% patients with AML accompanied lymphocyte antigen. In conclusion, leukemia immunophenotyping by three-color direct immunofluore staining methods could define some particular types of leukemia with an important value in diagnosis, treatment and predicting prognosis of acute leukemia.
Hospital 307, Academy of Military Medical Sciences, Beijing 100039, China.
Zhi-Juan
ZJ
Yao
Li
L
Liao
Yong-Hui
YH
Dang
Jing
J
Zhang
Ying
Y
Tian
Kai
K
Feng
Cong-Yan
CY
Qi
Jing-Feng
JF
Wang
Hu
H
Chen
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis; D051925:CD79 Antigens; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D015470:Leukemia, Myeloid, Acute / Q000276:immunology*; D017493:Leukocyte Common Antigens / Q000032:analysis; D008297:Male; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000032:analysis
D015703:Antigens, CD; D051925:CD79 Antigens; C497881:CD79A protein, human; D011947:Receptors, Antigen, B-Cell; D017493:Leukocyte Common Antigens