Rhamnolipid production by Pseudomonas aeruginosa immobilised in polyvinyl alcohol beads.
A marine bacterium, Pseudomonas aeruginosa BYK-2 (KCTC 18012P), was immobilised by entrapment in 10% (w/v) polyvinyl alcohol beads and optimized for the continuous production of rhamnolipid. The relative activity of rhamnolipid production was maintained at 80 approximately 90% of the initial production during 15 cycles in a repeated batch culture. Continuous culture was performed in a 1.8 1 airlift bioreactor, yielding 0.1 g rhamnolipid h(-1) at a dilution rate of 0.0 18 h(-1), 25 degrees C, initial pH 7, and 0.5 vvm aeration rate with a 1.21 working volume.
Department of Biotechnology and Bioengineering, Pukyong National University 599-1 Daeyeon-dong, Nam-gu, Pusan 608-737, Korea.
Hye-Sung
HS
Jeong
Dong-Jung
DJ
Lim
Sun-Hee
SH
Hwang
Soon-Duck
SD
Ha
Jai-Yul
JY
Kong
D019149:Bioreactors / Q000382:microbiology*; D002244:Carbon / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D018914:Cells, Immobilized / Q000502:physiology; D006017:Glycolipids / Q000096:biosynthesis*; D008863:Microspheres; D009584:Nitrogen / Q000378:metabolism; D011142:Polyvinyl Alcohol; D011550:Pseudomonas aeruginosa / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*
D006017:Glycolipids; C418382:rhamnolipid; D002244:Carbon; D011142:Polyvinyl Alcohol; D009584:Nitrogen