Syringe-cartridge solid-phase extraction method for patulin in apple juice.
A syringe-cartridge solid-phase extraction (SPE) method was developed for determination of patulin in apple juice. A 2.5 mL portion of test sample was passed through a conditioned macroporous SPE cartridge and washed with 2 mL 1% sodium bicarbonate followed by 2 mL 1% acetic acid. Patulin was eluted with 1 mL 10% ethyl acetate in ethyl ether and determined by reversed-phase liquid chromatography using a mobile phase consisting of 81% acetonitrile, 9% water, and 10% 0.05M potassium phosphate buffer, pH 2.4. Recoveries averaged 92% and the relative standard deviation was 8.0% in test samples spiked with 50 ng/mL patulin. The method appears to be applicable for monitoring apple juice samples to meet the U.S. Food and Drug Administration compliance action level of 50 microg/kg in an industrial quality assurance laboratory environment.
Eisele
Thomas A
TA
Tree Top Inc., PO Box 248, 111 S. Railroad Ave, Selah, WA 98942, USA. teisele@treetop.com
Gibson
Midori Z
MZ
D001628:Beverages / Q000032:analysis*; D002273:Carcinogens / Q000032:analysis*; D002853:Chromatography, Liquid; D005374:Filtration; D007202:Indicators and Reagents; D027845:Malus / Q000737:chemistry*; D010365:Patulin / Q000032:analysis*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D002273:Carcinogens; D007202:Indicators and Reagents; D012997:Solvents; D010365:Patulin