Oxidatively damaged proteins of heart mitochondrial electron transport complexes.
Protein modifications, such as carbonylation, nitration and formation of lipid peroxidation adducts, e.g. 4-hydroxynonenal (HNE), are products of oxidative damage attributed to reactive oxygen species (ROS). The mitochondrial respiratory chain Complexes I and III have been shown to be a major source of ROS in vitro. Additionally, modifications of the respiratory chain Complexes (I-V) by nitration, carbonylation and HNE adduct decrease their enzymatic activity in vitro. However, modification of these respiratory chain complex proteins due to in vivo basal level ROS generation has not been investigated. In this study, we show a basal level of oxidative damage to specific proteins of adult bovine heart submitochondrial particle (SMP) complexes, and find that most of these proteins are localized in the mitochondrial matrix. We postulate that electron leakage from respiratory chain complexes and subsequent ROS formation may cause damage to specific complex subunits and contribute to long-term accumulation of mitochondrial dysfunction.
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, TX 77550-0643, USA.
K B
KB
Choksi
W H
WH
Boylston
J P
JP
Rabek
W R
WR
Widger
J
J
Papaconstantinou
D033741:Adenine Nucleotide Translocator 1 / Q000032:analysis; D000447:Aldehydes / Q000737:chemistry; D000818:Animals; D002417:Cattle; D045222:Electron Transport Chain Complex Proteins / Q000032:analysis / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D007425:Intracellular Membranes / Q000201:enzymology; D008929:Mitochondria, Heart / Q000201:enzymology*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D018272:Porins / Q000032:analysis; D017434:Protein Structure, Tertiary; D017382:Reactive Oxygen Species / Q000737:chemistry / Q000378:metabolism; D013367:Submitochondrial Particles / Q000201:enzymology; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000737:chemistry; D050994:Voltage-Dependent Anion Channels
D033741:Adenine Nucleotide Translocator 1; D000447:Aldehydes; D045222:Electron Transport Chain Complex Proteins; D018272:Porins; D017382:Reactive Oxygen Species; D050994:Voltage-Dependent Anion Channels; C002744:3-nitrotyrosine; D014443:Tyrosine; C027576:4-hydroxy-2-nonenal