Proteomic fingerprinting of human immunodeficiency virus type 1-associated dementia from patient monocyte-derived macrophages: A case study.
The emergence of a subset of circulating monocytes during human immunodeficiency virus type 1 (HIV-1) disease has been shown to correlate with cognitive impairment. Thus, it is hypothesized that diagnostic protein profiles may be obtained from these cells from patients with or at risk for HIV-1-associated dementia (HAD). To address this possibility, we used ProteinChip assays to define a unique monocyte-derived macrophage (MDM) protein fingerprint during HAD and whether it is affected by highly active antiretroviral therapy (HAART). The study included five Hispanic women, one with HAD, two HIV-1-infected without cognitive impairment, and two seronegative controls. All patients were matched by age and immune status. Monocytes were recovered from the peripheral blood leukocytes by Percoll gradient centrifugation and allowed to differentiate in vitro for 7 days. Cell lysates and supernatants were collected from the MDM and analyzed by surface enhanced laser desorption/ionization-time of flight ProteinChip assays. Seven unique protein peaks between 3.0 and 20.0 kDa were found in the HAD MDM sample. Each of these proteins were abrogated after HAART. Additional studies extending this one time point determination would serve to confirm the general utility of MDM protein profiling for the diagnosis and monitoring of HAD.
The Departments of Microbiology and Specialized NeuroSciences Program, University of Puerto Rico Medical Sciences Campus, San Juan, Puerto Rico.
Valerie
V
Wojna
Kimberly A
KA
Carlson
Xiaoguang
X
Luo
Raúl
R
Mayo
Loyda M
LM
Meléndez
Edmundo
E
Kraiselburd
Howard E
HE
Gendelman
D015526:AIDS Dementia Complex / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology*; D000328:Adult; D015415:Biomarkers; D005260:Female; D015497:HIV-1; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000502:physiology* / Q000821:virology*; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology; D040081:Protein Array Analysis; D040901:Proteomics
D015415:Biomarkers