Fluorescence-labeled sphingosines as substrates of sphingosine kinases 1 and 2.
Fluorescently labeled d-erythro-sphingosines have been successfully synthesized in 9 and 12 steps starting from commercially available Garner aldehyde. Determining the influence of the nature, position and linkage of the label on the in vitro phosphorylation rate by sphingosine kinases 1 and 2 resulted in the identification of a pyrene- and a NBD-labeled sphingosine which are both phosphorylated with efficiency comparable to the natural substrate.
Novartis Institute for Biomedical Research, Brunnerstrasse 59, Vienna A-1235, Austria. peter.ettmayer@pharma.novartis.com
Peter
P
Ettmayer
Andreas
A
Billich
Thomas
T
Baumruker
Diana
D
Mechtcheriakova
Heide
H
Schmid
Peter
P
Nussbaumer
D002460:Cell Line; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D013110:Sphingosine / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity; D000099157:Sphingosine Kinase
D005456:Fluorescent Dyes; D017853:Phosphotransferases (Alcohol Group Acceptor); D013110:Sphingosine; D000099157:Sphingosine Kinase