Improved antigenic methods for differential diagnosis of bovine, caprine, and cervine alphaherpesviruses related to bovine herpesvirus 1.
The control of infectious bovine rhinotracheitis induced by bovine herpesvirus 1 (BoHV-1) requires sensitive and specific diagnostic assays. As BoHV-1 is antigenically and genetically related to four other alphaherpesviruses of ruminants-namely, BoHV-5, caprine herpesvirus 1 (CpHV-1), cervine herpesvirus 1 (CvHV-1) and CvHV-2-diagnostic tests able to discriminate BoHV-1 from these related viruses are needed to avoid misdiagnosis, especially because some of these viruses are able to cross the species barrier. In this study, murine monoclonal antibodies (MAbs) specific for BoHV-1, BoHV-5, CpHV-1, CvHV-1, and CvHV-2 were produced with the aim of setting up an immunofluorescence assay able to discriminate between these related herpesviruses. Produced MAbs were selected for their viral specificity by enzyme-linked immunosorbent assay and indirect immunofluorescence staining of virus-infected cells. Radioimmunoprecipitation characterization of the selected MAbs revealed that four of them are directed against glycoprotein C (gC) and one of them is directed against gD of these related viruses. The obtained results demonstrate that the antibodies produced allow an unambiguous discrimination of each of the four alphaherpesviruses related to BoHV-1.
Department of Infectious and Parasitic Diseases, Laboratory of Virology and Immunology, Faculty of Veterinary Medicine, University of Liège, B 4000 Liège, Belgium.
Véronique
V
Keuser
Frédéric
F
Schynts
Bruno
B
Detry
Alfred
A
Collard
Béatrice
B
Robert
Alain
A
Vanderplasschen
Paul-Pierre
PP
Pastoret
Etienne
E
Thiry
D018138:Alphaherpesvirinae / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000821:virology; D002460:Cell Line; D003670:Deer; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay; D015511:Goat Diseases / Q000175:diagnosis / Q000821:virology; D006041:Goats; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary; D007242:Herpesvirus 1, Bovine / Q000276:immunology / Q000302:isolation & purification*; D007668:Kidney; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral