Fabrication of microbial chip using collagen gel microstructure.
A microbial chip was fabricated by filling the micropores on a glass substrate with collagen-embedded Escherichia coli(E. coli) cells, and characterized by scanning electrochemical microscopy (SECM) in a solution containing ferricyanide. The activity of the E. coli cells in the collagen gel microstructure was imaged and characterized with SECM by mapping the localized concentration of ferrocyanide produced by the respiration of the cells. The SECM-based activity measurement detected as low as approximately 100 E. coli cells. Furthermore, the optical-microscopic observation indicated that the E. coli cells on the chip proliferated during the incubation. The sequential SECM measurements were performed for the same E. coli chip to obtain the microbial growth curve for a small number of microorganisms.
Kaya
Takatoshi
T
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai 980-8579, Japan.
Nagamine
Kuniaki
K
Oyamatsu
Daisuke
D
Shiku
Hitoshi
H
Nishizawa
Matsuhiko
M
Matsue
Tomokazu
T
D003094:Collagen / Q000737:chemistry*; D004563:Electrochemistry; D004926:Escherichia coli / Q000737:chemistry* / Q000254:growth & development; D005292:Ferricyanides / Q000378:metabolism; D005782:Gels; D008838:Microcomputers; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology; D010084:Oxidation-Reduction
D005292:Ferricyanides; D005782:Gels; C007931:hexacyanoferrate III; D003094:Collagen