Validation of a method for simultaneous determination of menthol and methyl salicylate in pharmaceuticals by capillary gas chromatography with cool on-column injection.
The conditions for the identification and quantitative determination of menthol and methyl salicylate in ointment Balsamum Mentholi Compositum on a hydrocarbon-ester base (vaseline-lanolin), have been established by using capillary gas chromatography with cool on-column injection and flame ionization detection (FID). The good separation of menthol (tR = 7.2 min), methyl salicylate (tR = 8.7 min) and thymol (tR = 12.3 min) and camphor (tR = 6.0 min), used as alternative internal standards, beside vehiculum constituents (peak of tR = 15.8 min) was obtained. The method features a high sensitivity - detection limit for menthol and methyl salicylate was 0.1 ng and 5.0 ng, respectively, high accuracy, precision and recovery for active substances: 100.0% +/- 2.2%, when camphor was used as an internal standard.
Department of Inorganic and Analytical Chemistry, Collegium Medicum of the Jagiellonian University, 9 Medyczna Str., 30-688 Kraków, Poland. jankrzek@cm-uj.krakow.pl
Jan
J
Krzek
Janusz Sławomir
JS
Czekaj
Włodzimierz
W
Rzeszutko
D002849:Chromatography, Gas / Q000379:methods; D008610:Menthol / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D012459:Salicylates / Q000032:analysis*; D012680:Sensitivity and Specificity
D004364:Pharmaceutical Preparations; D012459:Salicylates; D008610:Menthol; C033069:methyl salicylate