50 Hz magnetic fields activate mussel immunocyte p38 MAP kinase and induce HSP70 and 90.
Fifty hertz magnetic fields (MFs) induced the expression of heat shock proteins (HSPs) 70 and 90 in immunocytes of the mussel Mytilus galloprovincialis. Animals exposed at 300 microT for three different times (30; 2 x 30; 3 x 30 min), did not show differences in the HSP densitometric values in comparison with non-exposed mussels. At 400 microT, exposed animals showed a time-dependent increase in HSP expression as revealed by Western blot. After exposure to 600 microT, the HSP densitometric values were significantly higher than controls but not related to exposure duration. The induction of HSPs is concomitant with the activation of p38 MAP kinase signalling pathway. The present findings suggest the possibility to modulate the expression of HSPs by an appropriate time-intensity magnetic field exposure.
Department of Animal Biology, University of Modena and Reggio Emilia, via Campi 213/D, 41100 Modena, Italy.
Davide
D
Malagoli
Mauro
M
Lusvardi
Fabriziomaria
F
Gobba
Enzo
E
Ottaviani
D000818:Animals; D049872:Bivalvia / Q000166:cytology / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D004789:Enzyme Activation / Q000528:radiation effects; D015870:Gene Expression / Q000528:radiation effects; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000528:radiation effects; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis* / Q000528:radiation effects; D015151:Immunoblotting; D008214:Lymphocytes / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism* / Q000528:radiation effects; D048051:p38 Mitogen-Activated Protein Kinases
D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases