Cdk inhibitor ste9p/srw1p is involved in response to protein synthesis inhibition in fission yeast.
It remains unknown whether the cell cycle system responds properly to protein synthesis inhibition. In this paper I report finding in Schizosaccharomyces pombe that partially deleted elongation factor 3 genes rescue various mitotic catastrophe mutants depending on deltaste9 in a dominant-negative manner. In response to protein synthesis inhibitors, deltaste9 and some other mutants delay halting the cell cycle at G2-M and the combined cdc2-M26 deltaste9 mutant greatly loses viability. It is suggested that cell cycle be positively controlled in an ste9-dependent manner before essential factors for viability and other important functions are exhausted when protein synthesis is inhibited.
Department of Biochemistry and Molecular Biology, Graduate School of Medicine, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan. tsakai@m.u-tokyo.ac.jp
Takaaki
T
Sakai
D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors* / Q000235:genetics; D003513:Cycloheximide / Q000494:pharmacology; D005434:Flow Cytometry; D005656:Fungal Proteins / Q000502:physiology; D016195:G2 Phase / Q000187:drug effects / Q000502:physiology; D017353:Gene Deletion; D018816:Genes, cdc; D006921:Hygromycin B / Q000494:pharmacology; D016296:Mutagenesis; D010445:Peptide Elongation Factors / Q000502:physiology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D013489:Suppression, Genetic
D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D010445:Peptide Elongation Factors; D011500:Protein Synthesis Inhibitors; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C111584:YEF3 protein, S cerevisiae; C113000:srw1 protein, S pombe; D006921:Hygromycin B; D003513:Cycloheximide; D018844:Cyclin-Dependent Kinases