[A study of the dynamic relationship between AVP mRNA expression in PVN and immuno-responsiveness in rats].
OBJECTIVE
To investigate the proper dynamic relationship between AVP mRNA expression in PVN and immune response.


METHODS
Through the establishment of the immuno-enhancement and immunosuppression rat models, the in situ hybridization histochemical methods and the images analytical techniques were used to detect the dynamic variations of AVP mRNA content of PVN during different periods of immune response.


RESULTS
1. The average area of AVP mRNA positive signal in single cell of PVN in the immune suppression group was significantly smaller than that in the immune enhancement group and control group (P < 0.05). 2. The average gray degree of AVP mRNA positive signal in single cell of PVN in the maximum period of immune response was lower than that in the incipient enhancement period of immune response (P < 0.05). On the other hand, the average gray degree was clearly higher in the minimum period of immune response than that in the incipient suppression period of immune response (P < 0.05). 3. The total area that the AVP mRNA-positive signal occupied in the whole PVN in the maximum enhancement period of immune response was significantly larger than that in the incipient enhancement period response and the control group (P < 0.05). Conversely, it was evidently smaller in the minimum suppression period than in the incipient suppression period of immune response (P < 0.05). 4. The average gray degree of AVP mRNA positive signal in the whole PVN in the maximum enhancement period of immune response was significantly lower than that in the incipient enhancement period and the control group (P < 0.05). In turn, it was obviously higher in the minimum suppression period than in the incipient suppression period of immune response and the control group (P < 0.05).


CONCLUSIONS
There is a positive relationship between the AVP mRNA content of PVN and the intensity of immune responsiveness, indicating that the situation of AVP gene transcription varies with the variation of immune responsiveness.
Department of Histology and Embryology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Jia-yuan
JY
Zhu
Ke-qun
KQ
Ou
Lei
L
Wang
Wen-yu
WY
Chen
Yu-qiong
YQ
Ma
D000818:Animals; D001127:Arginine Vasopressin / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D007109:Immunity; D016867:Immunocompromised Host; D017403:In Situ Hybridization; D008297:Male; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D012333:RNA, Messenger; D001127:Arginine Vasopressin