Peroxidase catalyzed formation of azine pigments--a convenient and sensitive method for the identification of human cells with positive myeloperoxidase reactivity.
A new and efficient method for the identification of human cells with positive myeloperoxidase reactivity was developed. This method is based on the myeloperoxidase catalyzed formation of p-naphthoquinone-thiazol-2-on-azine pigments and offers a number of advantages compared to standard methods. In addition, we report the horseradish peroxidase (HRP) catalyzed oxidative coupling of 2-hydrazono-4-thiazolines with alpha-naphthol. This transformation allows a new and efficient synthesis of p-naphthoquinone-thiazol-2-on-azines under mild conditions.
Institut für Chemie und Biochemie der Ernst-Moritz-Arndt-Universität Greifswald, Soldmannstr. 16, D-17487 Greifswald, Germany.
Anja
A
Bodtke
Wolf-Diethard
WD
Pfeiffer
Norbert
N
Ahrens
Peter
P
Langer
D002384:Catalysis; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000201:enzymology; D009000:Monocytes / Q000166:cytology / Q000201:enzymology; D009285:Naphthoquinones / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism*; D010860:Pigments, Biological / Q000378:metabolism*; D012680:Sensitivity and Specificity
D009285:Naphthoquinones; D010860:Pigments, Biological; D009195:Peroxidase