Identification of the chicken MARCKS phosphorylation site specific for differentiating neurons as Ser 25 using a monoclonal antibody and mass spectrometry.
MARCKS is an actin-modulating protein that can be phosphorylated in multiple sites by PKC and proline-directed kinases. We have previously described a phosphorylated form of this protein specific for differentiating chick neurons, detected with mAb 3C3. Here, we show that this antibody binds to MARCKS only when it is phosphorylated at Ser 25. These and previous data provide hints for a possible answer to the question of why this ubiquitous protein seems to be essential only for neural development.
Zolessi
Flavio R
FR
Laboratorio de Cultivo de Tejidos, Sección Biología Celular, DBCM, Facultad de Ciencias, Universidad de la República, Uruguay.
Durán
Rosario
R
Engström
Ulla
U
Cerveñansky
Carlos
C
Hellman
Ulf
U
Arruti
Cristina
C
D000818:Animals; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D001921:Brain / Q000166:cytology / Q000196:embryology; D002454:Cell Differentiation; D002642:Chick Embryo; D047908:Intracellular Signaling Peptides and Proteins; D008074:Lipoproteins / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology; D013058:Mass Spectrometry / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D009474:Neurons / Q000737:chemistry / Q000166:cytology; D010750:Phosphoproteins; D010766:Phosphorylation; D012160:Retina / Q000737:chemistry / Q000166:cytology / Q000196:embryology; D012694:Serine / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D047908:Intracellular Signaling Peptides and Proteins; D008074:Lipoproteins; D008565:Membrane Proteins; D010750:Phosphoproteins; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D012694:Serine