Differential expression of Desulfovibrio vulgaris genes in response to Cu(II) and Hg(II) toxicity.
The response of Desulfovibrio vulgaris to Cu(II) and Hg(II) was characterized. Both metals increased the lag phase, and Cu(II) reduced cell yield at concentrations as low as 50 microM. mRNA expression was analyzed using random arbitrarily primed PCR, differential display, and quantitative PCR. Both Cu(II) and Hg(II) (50 micro M) caused upregulation of mRNA expression for an ATP binding protein (ORF2004) and an ATPase (ORF856) with four- to sixfold increases for Hg(II) and 1.4- to 3-fold increases with Cu(II). These results suggest that D. vulgaris uses an ATP-dependent mechanism for adapting to toxic metals in the environment.
Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019-0245, USA.
In Seop
IS
Chang
Jennifer L
JL
Groh
Matthew M
MM
Ramsey
Jimmy D
JD
Ballard
Lee R
LR
Krumholz
D001483:Base Sequence; D003300:Copper / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics; D016969:Desulfovibrio vulgaris / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D005798:Genes, Bacterial; D008628:Mercury / Q000494:pharmacology*; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019105:Random Amplified Polymorphic DNA Technique
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012333:RNA, Messenger; D003300:Copper; D008628:Mercury