Genetic diversity of mumps virus in oral fluid specimens: application to mumps epidemiological study.
Three hundred nine mumps virus (MuV) strains detected in the United Kingdom during 1995-2002 were characterized by partial sequencing of the small hydrophobic gene and were shown to belong to at least 6 different genotypes. A strain representing a new genotype was isolated from a seminal fluid specimen. Identical strains belonging to genotypes G and C were found to circulate for up to 3 years. One genotype H strain reappeared after an absence of 4 years. Distinct lineages (G1-G7) within genotype G were recognizable in the present study, and this level of characterization proved to be very useful for tracking MuV importations and subsequent transmission. We propose, here, a preliminary standardization of international nomenclature for genetic characterization of MuV strains, to facilitate future molecular epidemiological studies of mumps. Oral fluid (OF) specimens (n=1441) were used to detect both anti-MuV IgM and MuV genome, and the results indicate that OF specimens can be used successfully for diagnosis and have the potential to play a valuable role in diagnosis and surveillance of mumps.
Jin
Li
L
Enteric, Respiratory, and Neurological Virus Laboratory, Communicable Disease Surveillance Center, Health Protection Agency, London, United Kingdom. li.jin@hpa.org.uk
Brown
David W G
DW
Litton
Pam A
PA
White
Joanne M
JM
D001483:Base Sequence; D014644:Genetic Variation; D005838:Genotype; D015707:HN Protein / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009055:Mouth / Q000821:virology*; D009109:Mumps virus / Q000145:classification / Q000235:genetics*; D009154:Mutation; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction
D015707:HN Protein