Crystallization and preliminary X-ray analysis of a novel Trichoderma reesei xylanase IV belonging to glycoside hydrolase family 5.
Xylanase IV (XYN IV) is a new recently characterized xylanase from Trichoderma reesei. It is able to degrade several different xylans, mainly producing xylose. XYN IV has been crystallized by the hanging-drop vapour-diffusion method, using PEG 6000 as a precipitant. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 86.3, b = 137.5, c = 196.1 A, alpha = beta = gamma = 90 degrees. Assuming a molecular weight of 50.3 kDa, the V(M) values indicate there to be four XYN IV monomers in an asymmetric unit and the solvent content of the crystals to be 57%. Based on dynamic light-scattering measurements, XYN IV is a dimer in solution. A native data set to 2.8 A resolution has been collected at a home laboratory and a data set to 2.2 A resolution has been collected using synchrotron radiation.
Parkkinen
Tarja
T
Department of Chemistry, University of Joensuu, PO Box 111, FIN-80101 Joensuu, Finland. tarja.parkkinen@joesuu.fi
Hakulinen
Nina
N
Tenkanen
Maija
M
Siika-aho
Matti
M
Rouvinen
Juha
J
D003460:Crystallization; D018360:Crystallography, X-Ray; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry* / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism; D014242:Trichoderma / Q000201:enzymology*; D014990:Xylans / Q000378:metabolism
D005656:Fungal Proteins; D014990:Xylans; D043364:Endo-1,4-beta Xylanases