Insight into the genome of Aspergillus fumigatus: analysis of a 922 kb region encompassing the nitrate assimilation gene cluster.
Aspergillus fumigatus is the most ubiquitous opportunistic filamentous fungal pathogen of human. As an initial step toward sequencing the entire genome of A. fumigatus, which is estimated to be approximately 30 Mb in size, we have sequenced a 922 kb region, contained within 16 overlapping bacterial artificial chromosome (BAC) clones. Fifty-four percent of the DNA is predicted to be coding with 341 putative protein coding genes. Functional classification of the proteins showed the presence of a higher proportion of enzymes and membrane transporters when compared to those of Saccharomyces cerevisiae. In addition to the nitrate assimilation gene cluster, the quinate utilisation gene cluster is also present on this 922 kb genomic sequence. We observed large scale synteny between A. fumigatus and Aspergillus nidulans by comparing this sequence to the A. nidulans genetic map of linkage group VIII.
Pain
Arnab
A
The Pathogen Sequencing Unit, The Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridge CB10 1SA, UK.
Woodward
John
J
Quail
Michael A
MA
Anderson
Michael J
MJ
Clark
Richard
R
Collins
Matthew
M
Fosker
Nigel
N
Fraser
Audrey
A
Harris
David
D
Larke
Natasha
N
Murphy
Lee
L
Humphray
Sean
S
O'Neil
Susan
S
Pertea
Mihaela
M
Price
Claire
C
Rabbinowitsch
Ester
E
Rajandream
Marie-Adele
MA
Salzberg
Steven
S
Saunders
David
D
Seeger
Kathy
K
Sharp
Sarah
S
Warren
Tim
T
Denning
David W
DW
Barrell
Bart
B
Hall
Neil
N
D001232:Aspergillus fumigatus / Q000235:genetics* / Q000378:metabolism*; D001233:Aspergillus nidulans / Q000235:genetics; D022202:Chromosomes, Artificial, Bacterial; D004271:DNA, Fungal / Q000737:chemistry / Q000302:isolation & purification; D021901:DNA, Intergenic; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology; D023061:Gene Order; D016681:Genome, Fungal; D023281:Genomics; D007438:Introns; D026901:Membrane Transport Proteins / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D005810:Multigene Family; D009566:Nitrates / Q000378:metabolism*; D016366:Open Reading Frames; D011801:Quinic Acid / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017422:Sequence Analysis, DNA; D026801:Synteny
D004271:DNA, Fungal; D021901:DNA, Intergenic; D004798:Enzymes; D005656:Fungal Proteins; D026901:Membrane Transport Proteins; D009566:Nitrates; D011801:Quinic Acid