Some precautions in using chelators to buffer metals in biological solutions.
Chelators and associated computer programs are commonly used to buffer metal ions in biological experiments. This communication discusses common misunderstandings and pitfalls in use of these buffers and provides information on choosing the best metal buffer for different experimental situations.
Patton
Chris
C
Hopkins Marine Station, Stanford University, Pacific Grove, CA 93950, USA. cpatton@stanford.edu
Thompson
Stuart
S
Epel
David
D
D002021:Buffers; D002614:Chelating Agents / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000737:chemistry*; D008956:Models, Chemical; D012996:Solutions / Q000737:chemistry
D002021:Buffers; D002614:Chelating Agents; D008670:Metals; D012996:Solutions