Patensin-induced apoptosis is accompanied by decreased bcl-2 expression and telomerase activity in HL-60 cells.
The present study aimed to investigate the effect of telomerase activity in patensin-induced apoptosis and the regulation of B cell leukemia/lymphoma 2 (bcl-2) gene expression in human leukemia HL-60 cells. Apoptosis of HL-60 cells was induced by patensin (100 micromol L(-1)) for 3, 6, 12 and 24 h. Apoptosis and bcl-2 were determined by flow cytometry analysis. A polymerase-chain-reaction-based telomeric repeat amplification protocol assay was used to detect the telomerase activity. Patensin induced growth arrest and apoptotic cell death in HL-60 cells. The telomerase activity was inhibited in a time-dependent manner during the patensin-induced apoptosis of HL-60 cells, and the expression of bcl-2 was progressively down-regulated by patensin. Inhibition of the telomerase activity of HL-60 cells was closely related to the patensin-induced apoptosis. The present results indicate that inhibition in telomerase and reduced bcl-2 gene expression may play a role in the patensin-induced apoptosis of HL-60 cells.
Department of Oncology, Yijishan Hospital, Wannan Medical College, Wuhu, Anhui 241001, China. jzning@ust.hk
Zhao-Ning
ZN
Ji
Wen-Cai
WC
Ye
Jing
J
Rui
Lu
L
Wang
Guo-Qing
GQ
Liu
D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D019254:Genes, bcl-2; D018922:HL-60 Cells; D006801:Humans; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D012503:Saponins / Q000494:pharmacology*; D019098:Telomerase / Q000378:metabolism*
D012503:Saponins; C094961:patensin; D009828:Oleanolic Acid; D019098:Telomerase