Essential function of oncostatin m in nociceptive neurons of dorsal root ganglia.
Oncostatin M (OSM) is a member of the interleukin-6 family of cytokines, and we have reported previously that the murine OSM receptor beta subunit (OSMR) was expressed in some neurons in the adult trigeminal and dorsal root ganglia (DRGs) and in the perineonatal hypoglossal nucleus. In the present study, we investigated the development of OSMR-positive neurons of DRGs in OSM-deficient mice. In situ hybridization revealed that OSMR-positive neurons in DRGs began to appear at postnatal day 0 (P0) and reached the adult level at P14. In the DRGs of the OSM-deficient mice, vanilloid receptor 1 (VR1)- and P2X3-positive small-sized neurons were significantly decreased. In addition, OSMR-positive neurons decreased, resulting in the reduced number of VR1/P2X3/OSMR-triple positive neurons. OSM-deficient mice displayed significantly reduced noxious responses in models of acute thermal, mechanical, chemical, and visceral pain. Thus, OSM plays an essential role in the development of a subtype of nociceptive neurons in the DRGs.
Morikawa
Yoshihiro
Y
Department of Anatomy and Neurobiology, Wakayama Medical University, Wakayama, 641-8509 Japan. yoshim@wakayama-med.ac.jp
Tamura
Shinobu
S
Minehata
Ken-ichi
K
Donovan
Peter J
PJ
Miyajima
Atsushi
A
Senba
Emiko
E
D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis; D001522:Behavior, Animal; D002452:Cell Count; D048429:Cell Size; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018390:Gene Targeting; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D053683:Oncostatin M; D010146:Pain / Q000378:metabolism*; D010147:Pain Measurement; D010455:Peptides / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D018121:Receptors, Cytokine / Q000096:biosynthesis* / Q000235:genetics; D011955:Receptors, Drug / Q000096:biosynthesis; D053677:Receptors, Oncostatin M; D018048:Receptors, Purinergic P2 / Q000096:biosynthesis; D058477:Receptors, Purinergic P2X3
D000943:Antigens, Differentiation; C508352:Osm protein, mouse; C546409:P2rx3 protein, mouse; D010455:Peptides; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D011955:Receptors, Drug; D053677:Receptors, Oncostatin M; D018048:Receptors, Purinergic P2; D058477:Receptors, Purinergic P2X3; D053683:Oncostatin M