A kinesin-like motor inhibits microtubule dynamic instability.
The motility of molecular motors and the dynamic instability of microtubules are key dynamic processes for mitotic spindle assembly and function. We report here that one of the mitotic kinesins that localizes to chromosomes, Xklp1 from Xenopus laevis, could inhibit microtubule growth and shrinkage. This effect appeared to be mediated by a structural change in the microtubule lattice. We also found that Xklp1 could act as a fast, nonprocessive, plus end-directed molecular motor. The integration of the two properties, motility and inhibition of microtubule dynamics, in one molecule emphasizes the versatile properties of kinesin family members.
Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrabetae 1, 69117 Heidelberg, Germany.
Henrik
H
Bringmann
Georgios
G
Skiniotis
Annina
A
Spilker
Stefanie
S
Kandels-Lewis
Isabelle
I
Vernos
Thomas
T
Surrey
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000266:Adenylyl Imidodiphosphate / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D018385:Centrosome / Q000378:metabolism; D002875:Chromosomes / Q000378:metabolism; D020285:Cryoelectron Microscopy; D019281:Dimerization; D007700:Kinetics; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D020409:Molecular Motor Proteins / Q000378:metabolism*; D017239:Paclitaxel / Q000494:pharmacology; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism; D029867:Xenopus Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis
D008869:Microtubule-Associated Proteins; D020409:Molecular Motor Proteins; C452196:NAT14 protein, Xenopus; D011993:Recombinant Fusion Proteins; D014404:Tubulin; D029867:Xenopus Proteins; D000266:Adenylyl Imidodiphosphate; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D017239:Paclitaxel