Effects of obesity on the relationship of leptin mRNA expression and adipocyte size in anatomically distinct fat depots in mice.
In support of leptin's physiological role as humoral signal of fat mass, we have shown that adipocyte volume is a predominant determinant of leptin mRNA levels in anatomically distinct fat depots in lean young mice in the postabsorptive state. In this report, we investigated how obesity may affect the relationship between leptin mRNA levels and adipocyte volume in anatomically distinct fat depots in mice with genetic (Lep(ob)/Lep(ob) and A(y)/+), diet-induced, and aging-related obesity. In all of the obese mice examined, tissue leptin mRNA levels relative to the average adipocyte volume were lower in the perigonadal and/or retroperitoneal than in the inguinal fat depots and were lower than those of the lean young mice in the perigonadal fat depot. A close, positive correlation between leptin mRNA level and adipocyte volume was present from small to hypertrophic adipocytes within each perigonadal and inguinal fat pad in the obese mice, but the slopes of the regression lines relating leptin mRNA level to adipocyte volume were significantly lower in the perigonadal than in the inguinal fat pads of the same mice. These results suggest that obesity per se is associated with a decreased leptin gene expression per unit of fat mass in mice and that the positive correlation between leptin mRNA level and adipocyte volume is an intrinsic property of adipocytes that is not disrupted by adipocyte hypertrophy in obese mice.
Division of Molecular Genetics, Columbia Univ., College of Physicians and Surgeons, Russ Berrie Pavilion, 1150 St. Nicholas Ave., New York, NY 10032, USA.
Kai-Ying
KY
Guo
Patricia
P
Halo
Rudolph L
RL
Leibel
Yiying
Y
Zhang
D017667:Adipocytes / Q000502:physiology* / Q000648:ultrastructure*; D000273:Adipose Tissue / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D048429:Cell Size / Q000235:genetics / Q000502:physiology; D004032:Diet; D004041:Dietary Fats / Q000494:pharmacology; D020738:Leptin / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009765:Obesity / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D011965:Receptors, Glucocorticoid / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004041:Dietary Fats; D020738:Leptin; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid