Multiplex PCR assay for direct identification of group B streptococcal alpha-protein-like protein genes.
We developed a group B streptococcus multiplex PCR assay which allows, by direct analysis of the amplicon size, determination of the surface protein antigen genes of alpha-C protein, epsilon protein, Rib, Alp2, Alp3, and Alp4. The multiplex PCR assay offers a rapid and simple method of subtyping Streptococcus agalactiae based on surface protein genes.
Laboratorio di Batteriologia, Istituto Superiore di Sanità, 00161 Rome, Italy. roberta.creti@iss.it
Roberta
R
Creti
Francesca
F
Fabretti
Graziella
G
Orefici
Christina
C
von Hunolstein
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013292:Streptococcus agalactiae / Q000235:genetics* / Q000302:isolation & purification
D001426:Bacterial Proteins; D017931:DNA Primers