The osteoblast mechano-receptor, microgravity perception and thermodynamics.
Mechano-sensing in cells is tightly obliged with changes in intracellular free calcium (IFC), regulation of specific genes and activation of specific second messenger systems. To investigate whether single non-professional cells like osteoblasts can detect microgravity through the mechano-sensor, measurements on a sub-orbital rocket and parabolic flights observing the IFC and gene expression were performed. We find that microgravity did neither effect IFC nor gene expression. Thermal and mechanical noise within cells is too high in relation to the change of force due to the change from gravity to microgravity. Complementary force measurements have shown that cells exert high forces on the substrate and that these high forces have to be applied for activation.
Klopp
Erk
E
Department of Semiconductor Physics, University of Marburg, Marburg, Germany. Erk.Klopp@physik.uni-marburg.de
Graff
Daniel
D
Struckmeier
Jens
J
Born
Matthias
M
Curtze
Sami
S
Hofmann
Martin
M
Jones
David
D