Developmental stage-specific expression of Rbm suggests its involvement in early phases of spermatogenesis.
Rbm is a male infertility gene located on the Y chromosome that is expressed in the testis. To investigate the specific events of spermatogenesis in which Rbm plays a role, the precise pattern of expression of Rbm in the mouse testis was determined. An antibody was generated against the Rbm protein and used to detect a single specific band of 43 kDa in size in mouse testicular lysates. In situ hybridization, immunoblot and immunohistochemistry analyses together indicated that Rbm was expressed in spermatogonia, preleptotene spermatocytes, late leptotene to early pachytene spermatocytes but not in mid-pachytene spermatocytes or subsequent stages of differentiation, including haploid germ cells. These observations suggest that Rbm functions in early but not later stages of male germ cell development.
School of Biological Sciences, Seoul National University, Seoul 151-742, Korea.
Jungmin
J
Lee
Jinpyo
J
Hong
Euisu
E
Kim
Kyungjin
K
Kim
Soo Woong
SW
Kim
Hanumanthappa
H
Krishnamurthy
Sanny S W
SS
Chung
Debra J
DJ
Wolgemuth
Kunsoo
K
Rhee
D000818:Animals; D017930:Genes, Reporter; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009687:Nuclear Proteins; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D018168:Receptors, Retinoic Acid / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D000072482:Retinoic Acid Receptor alpha; D013087:Spermatids / Q000378:metabolism; D013091:Spermatogenesis / Q000502:physiology*
D009687:Nuclear Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C000607072:Rara protein, mouse; C501500:Rbmy protein, mouse; D018168:Receptors, Retinoic Acid; D011993:Recombinant Fusion Proteins; D000072482:Retinoic Acid Receptor alpha