Identification of two new HLA-B alleles, HLA-B*0732 and HLA-B*5809, by sequence-based typing.
Here, we have described the characterization of two novel human leukocyte antigen-B (HLA-B) alleles. The new alleles, HLA-B*0732 and HLA-B*5809, were identified in Italian Caucasian individuals. B*0732 differs from HLA-B*0708 by one nucleotidic change at position 412 (from G to A) in exon 3, leading to an amino acidic substitution from Asp (GAC) to Asn (AAC) at codon 114. The sequence of B*5809 is identical to that of HLA-B*5801, except for a point mutation at position 583 in exon 3, where a T is substituted by a C. This change leads to an amino acidic substitution from Tyr (TAC) to His (CAC) at codon 171.
Centro Trasfusionale e di Immunologia dei Trapianti, Ospedale Maggiore Policlinico IRCCS, Milano, Italy. nitpmi@policlinico.mi.it
E
E
Longhi
S
S
Frison
F
F
Poli
A
A
Espadas de Arias
C
C
Zanuso
E
E
Guizzardi
M
M
Scalamogna
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D015235:HLA-B Antigens / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D007558:Italy; D008969:Molecular Sequence Data; D017354:Point Mutation; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D044465:White People
D015235:HLA-B Antigens