A new steroidal saponin from Agave attenuata.
A new steroidal saponin was isolated from the leaves of Agave attenuata. Its structure was established as (3beta,beta,25S)-spirostan-3-yl O-beta-D-glucopyranosyl-(1 --> 2)-beta-D-glucopyranosyl-(1 --> 2)-O-[beta-D-glucopyranosyl-(1 --> 3)]-beta-D-glucopyranosyl-(1 --> 4)-beta-D-galactopyranoside. The structural identification was performed using detailed analyses of 1H- and 13C-NMR spectra including 2D NMR spectroscopic techniques (COSY, HETCOR, and COLOC) and chemical conversions. The hemolytic activity of the steroidal saponin was evaluated using an in vitro assay.
Laboratório de Química de Plantas Medicinais, Núcleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, PO Box 68045 CEP, 21944-970 Rio de Janeiro, Brazil.
Tatiana Paz
TP
Mendes
Graziela de Medeiros
Gde M
Silva
Bernadete Pereira
BP
da Silva
José Paz
JP
Parente
D027882:Agave / Q000737:chemistry*; D001681:Biological Assay; D018929:Cell Culture Techniques; D004912:Erythrocytes; D006461:Hemolysis; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D018515:Plant Leaves / Q000737:chemistry; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology
D012503:Saponins