Atypical apoptosis in L929 cells induced by evodiamine isolated from Evodia rutaecarpa.
Two alkaloids, evodiamine and rutaecarpine, isolated from the dried fruits of Evodia rutaecarpa Bentham were evaluated in vitro for antiproliferation activity on tumor cells versus human peripheral blood mononuclear cells (PBMC). Evodiamine had more potent cytotoxic effects on five tumor cell lines (human malignant melanoma A375-S2, human cervical cancer HeLa, human breast adenocarcinoma MCF7, human acute monocytic leukemia THP-1, murine fibrosarcoma L929) than rutaecarpine. Moreover, evodiamine did not affect PBMC viability for a 36 h culture period. Although apoptotic bodies were observed in evodiamine-treated L929 cells stained with Hoechst 33258, DNA fragmentation as a hallmark of apoptosis was not found. Caspases were involved in the protection of L929 cells against cell death. Evodiamine initiated atypical apoptosis in L929 cells by cycle arrest at the G0/G1 phase.
China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016, China.
Ying
Y
Zhang
Qi-Hui
QH
Zhang
Li-Jun
LJ
Wu
Shin-Ichi
S
Tashiro
Satoshi
S
Onodera
Takashi
T
Ikejima
D000230:Adenocarcinoma / Q000473:pathology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000473:pathology; D002470:Cell Survival; D032086:Evodia / Q000737:chemistry*; D005260:Female; D005354:Fibrosarcoma / Q000473:pathology; D005638:Fruit; D006801:Humans; D007948:Leukemia, Monocytic, Acute / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000502:physiology; D008545:Melanoma / Q000473:pathology; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D011799:Quinazolines / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000473:pathology
D010936:Plant Extracts; D011799:Quinazolines; C049639:evodiamine