Sequence-selective DNA detection using multiple laminar streams: a novel microfluidic analysis method.
On-site detection methods for DNA have been demanded in the pathophysiology field. Such analysis requires a simple and accurate method, rather than high-throughput. This report describes a novel microfluidic analysis method and its application for simple sequence-selective DNA detection. The method uses a microchannel device with a serpentine structure. Sequence-specific binding of probe DNA can be detected at one side of the microchannel. This method is capable of sequence-specific detection of DNA with high accuracy. Single base mutations can also be analyzed. Combination of laminar stream and laminar secondary flow in the microchannel enable specific detection of probe-bound DNA.
Yamashita
Kenichi
K
Micro-space Chemistry Laboratory, National Institute of Advanced Science and Technology, 807-1 Shuku-machi, Tosu, Saga 841-0052, Japan.
Yamaguchi
Yoshiko
Y
Miyazaki
Masaya
M
Nakamura
Hiroyuki
H
Shimizu
Hazime
H
Maeda
Hideaki
H
D001483:Base Sequence; D001665:Binding Sites; D017931:DNA Primers; D015342:DNA Probes; D005470:Fluorometry; D005821:Genetic Techniques / Q000295:instrumentation*; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D015342:DNA Probes