DNA amplification and rearrangements in archival Salmonella enterica serovar Typhimurium LT2 cultures.
Variations in genome size and gene order were observed in archival Salmonella enterica serovar Typhimurium cultures stored for over 40 years. In one strain, microarray analysis revealed a large, stable amplification. PCR analysis of the same strain revealed a genomic duplication that underwent a translocation. Other strains had smaller duplications and deletions. These results demonstrate that storage in stabs over time at room temperature not only allows for further bacterial growth but also may produce an environment that selects for a variety of mutations, including genomic rearrangements.
Sidney Kimmel Cancer Center, San Diego, California 92121, USA.
Steffen
S
Porwollik
Rita Mei-Yee
RM
Wong
R Allen
RA
Helm
Kelly K
KK
Edwards
Michael
M
Calcutt
Abraham
A
Eisenstark
Michael
M
McClelland
D018070:Biological Specimen Banks; D019143:Evolution, Molecular; D017353:Gene Deletion; D020440:Gene Duplication; D014644:Genetic Variation; D016680:Genome, Bacterial; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D012486:Salmonella typhimurium / Q000235:genetics* / Q000254:growth & development*; D014178:Translocation, Genetic; D012407:rRNA Operon