Determination of sulphite using an immobilized enzyme with flow injection chemiluminescence detection.
A flow injection method is reported for the determination of sulphite-based on chemiluminescent detection. Hydrogen peroxide is produced from sulphite using on-line covalently bound immobilized sulphite oxidase packed in a mini-column, which was mixed downstream and detected via cobalt(II)-catalysed chemiluminescent oxidation of luminol. The limit of detection (2 x standard deviation of the blank) was 1 x 10(-3) mmol/L with sample throughput 60 h(-1). The calibration data was linear over the range of 0.2-1.0 mmol/L with relative standard deviation (n = 4) in the range 0.9-2.0%.
Department of Chemistry, University of Balochistan, Quetta, Pakistan. yaqoob2001@hotmail.com
M
M
Yaqoob
A
A
Nabi
A
A
Waseem
M
M
Masoom-Yasinzai
D003035:Cobalt / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000378:metabolism*; D017022:Flow Injection Analysis / Q000379:methods*; D006861:Hydrogen Peroxide / Q000032:analysis; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000737:chemistry; D013447:Sulfites / Q000032:analysis*
D004800:Enzymes, Immobilized; D013447:Sulfites; D003035:Cobalt; D008165:Luminol; D006861:Hydrogen Peroxide; D050862:Oxidoreductases Acting on Sulfur Group Donors