A PKC wave follows the calcium wave after activation of Xenopus eggs.
Calcium waves are well-known hallmarks of egg activation that trigger resumption of the cell cycle and development of the embryo. These waves rapidly and efficiently assure that activation signals are transmitted to all regions of the egg. Although the mechanism by which the calcium wave propagates across an egg as large as that of Xenopus is not known, two models prevail. One model is a wave of calcium-induced calcium release (CICR) and the other is propagation by inositol-induced calcium release (IICR). IICR requires a wave of phosphatidylinositol 4,5-bisphosphate (PIP2) hydrolysis, generating two second messengers, IP3, which then releases calcium and DAG, which activates protein kinase C (PKC). We show here that a wave of PKC-green fluorescent protein travels across the egg immediately following, and at the same velocity as, the calcium wave. This is the first example of a PKC wave in a vertebrate egg and supports the IICR model of wave propagation.
Larabell
Carolyn A
CA
Department of AnatomyUniversity of California, San FranciscoSan Francisco, CA 94143, USA. larabel@itsa.ucsf.edu
Rowning
Brian A
BA
Moon
Randall T
RT
D000818:Animals; D020013:Calcium Signaling; D002478:Cells, Cultured; D004075:Diglycerides / Q000378:metabolism; D004625:Embryo, Nonmammalian; D004789:Enzyme Activation; D005260:Female; D049452:Green Fluorescent Proteins; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism / Q000502:physiology*; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism; D011493:Protein Kinase C / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology / Q000378:metabolism*; D015053:Zygote / Q000378:metabolism
D004075:Diglycerides; D008164:Luminescent Proteins; D019269:Phosphatidylinositol 4,5-Diphosphate; D049452:Green Fluorescent Proteins; D015544:Inositol 1,4,5-Trisphosphate; D011493:Protein Kinase C