The auxin conjugate 1-O-indole-3-acetyl-beta-D-glucose is synthesized in immature legume seeds by IAGlc synthase and may be used for modification of some high molecular weight compounds.
Immature seeds of some dicotyledonous plants contain IAGlc synthase catalysing the synthesis of 1-O-IAGlc. This enzyme activity is comparable with 1-O-IAGlc synthase activity investigated earlier in liquid endosperm of Zea mays. Polyclonal antibodies against maize 1-O-IAGlc synthase cross-react with partially purified 1-O-IAGlc synthase from immature pea and rape seeds. Single immunoreactive bands were observed at a locus corresponding to 45.7 kDa and 43.7 kDa from pea and rape enzyme preparations, respectively, unlike that from the 50 kDa molecular mass of the maize enzyme. It was also observed that some high molecular weight compounds of pea seeds are labelled in vivo by [(14)C] IAA, and unlabelled 1-O-IAGlc inhibits that labelling. In immature pea seeds 43-49.8% of the IAA-modified high molecular weight compounds, obtained after ultracentrifugation, was found in the soluble fraction and 50.1-57% in the insoluble fraction. Ester-linked IAA accounted for about 6-9% and 38-45.6% in soluble and insoluble material, respectively, estimated after hydrolysis in 1 N NaOH. Enzymatic hydrolysis of IAA-labelled high molecular weight compounds gives free IAA and compound(s) corresponding to IAGlc isomers. These results suggest that 1-O-IAGlc synthesized in legume seeds may be used for the modification of some high molecular weight compounds.
Jakubowska
Anna
A
Nicholas Copernicus University, Institute of General and Molecular Biology, Department of Biochemistry, ul. Gagarina 9, 87-100 Toruñ, Poland. anjakubo@cc.uni.torun.pl
Kowalczyk
Stanislaw
S
D029741:Brassica rapa / Q000201:enzymology; D007887:Fabaceae / Q000201:enzymology*; D005960:Glucosides / Q000378:metabolism*; D005964:Glucosyltransferases / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000096:biosynthesis* / Q000378:metabolism; D007211:Indoles / Q000378:metabolism*; D007700:Kinetics; D018532:Pisum sativum / Q000201:enzymology; D027805:Phaseolus / Q000201:enzymology; D010940:Plant Proteins / Q000378:metabolism; D012639:Seeds / Q000201:enzymology*; D013045:Species Specificity; D003313:Zea mays / Q000201:enzymology
C110292:1-O-(indole-3-acetyl)glucose; D005960:Glucosides; D007210:Indoleacetic Acids; D007211:Indoles; D010940:Plant Proteins; C030737:indoleacetic acid; D005964:Glucosyltransferases; C071643:indole-3-acetate beta-glucosyltransferase