Allele-specific binding to the -308 single nucleotide polymorphism site in the tumour necrosis factor-alpha promoter.
Single nucleotide polymorphisms in the tumour necrosis factor alpha (TNF-alpha) promoter region may modulate TNF-alpha gene transcriptional activity by modifying the binding of transcription factors. Here we confirm that a specific DNA complex binds preferentially the variant TNF2 allele in various cell types and demonstrate that activating protein (AP)-2, myeloid zinc finger gene 1 (MZF-1) and Sp1 are not involved in this complex.
Equipe d'Accueil 3737 Pathologie des Cellules Lymphoïdes, Claude Bernard University, Lyon, France.
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Baseggio
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Bartholin
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Chantome
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Charlot
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Rimokh
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Salles
D000483:Alleles; D001402:B-Lymphocytes / Q000378:metabolism; D001665:Binding Sites; D001667:Binding, Competitive; D002460:Cell Line; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D002470:Cell Survival; D004247:DNA / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D009000:Monocytes / Q000378:metabolism; D009841:Oligonucleotides / Q000737:chemistry; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D016329:Sp1 Transcription Factor / Q000235:genetics; D013601:T-Lymphocytes / Q000378:metabolism; D050656:Transcription Factor AP-2; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D004268:DNA-Binding Proteins; D051741:Kruppel-Like Transcription Factors; C085969:MZF1 protein, human; D009841:Oligonucleotides; D016329:Sp1 Transcription Factor; D050656:Transcription Factor AP-2; D014157:Transcription Factors; D014409:Tumor Necrosis Factor-alpha; D004247:DNA