[Study on the fingerprints of Achyranthes bidentata Bl. by HPLC/UV/MS].
OBJECTIVE
To establish the fingerprints of Achyranthes bidentata Bl. by HPLC/UV/MS.


METHODS
Separation was performed on Aglient Zorbax SB-C18 column. Gradient elute was performed by the mobile phase consisting of methanol and 0.1% formic acid-water with the flow rate of 1.0 ml/min.


RESULTS
Perfect fingerprints were obtained which can be used for the evaluation of Achyranthes bidentata Bl. Four common peaks were confirmed in fingerprints. Three compounds were elucidated as 5-hydroxymethyl furaldehyde, ecdysterone, inokosterone by HPLC/MS.


CONCLUSIONS
The method can be applied to the quality control of Achyranthes bidentata Bl.
Center for Instrumental Analysis, China Pharmaceutical University, Nanjing 210009.
Yuanyuan
Y
Wang
Zunjian
Z
Zhang
Xingwang
X
Wang
Xiangyang
X
Xu
Hui
H
Zhang
D029044:Achyranthes / Q000737:chemistry*; D002782:Cholestenes / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004441:Ecdysterone / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D010598:Pharmacognosy; D010946:Plants, Medicinal / Q000737:chemistry*; D011208:Powders; D011786:Quality Control; D013056:Spectrophotometry, Ultraviolet
D002782:Cholestenes; D011208:Powders; C100084:inokosterone; D004441:Ecdysterone