Analysis of MG63 osteoblastic-cell response to a new nanoporous implant surface by means of a microarray technology.
Surface implant modifications have been shown to have a relevant importance in modifying cell response. Expression profiling by DNA microarray is a new molecular technology that allows the analysis of gene expression in a cell system. By using DNA microarrays containing 19,200 genes, we identified in osteoblast-like cells line (MG-63) on new implant surface (nanoPORE, Out-Link, Sweden and Martina, Due Carrare, Padova, Italy), several genes whose expressions were significantly down-regulated. The differentially expressed genes cover a broad range of functional activities: (a) immunity, (b) vesicular transport (c) apoptosis and cell cycle regulation. It was also possible to detect some genes whose function is unknown. The data reported are, to our knowledge, the first genetic portrait of an implant surface. They can be relevant to better understand the molecular mechanism of implant osseointegration and as a model for comparing other materials.
Maxillofacial Surgery, University of Ferrara, Ferrara, Italy.
Francesco
F
Carinci
Furio
F
Pezzetti
Stefano
S
Volinia
Francesca
F
Francioso
Diego
D
Arcelli
Jlenia
J
Marchesini
Elisabetta
E
Caramelli
Adriano
A
Piattelli
D017209:Apoptosis / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics; D002460:Cell Line; D015921:Dental Implants; D015536:Down-Regulation; D020869:Gene Expression Profiling; D006801:Humans; D007109:Immunity / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D016348:Osseointegration / Q000235:genetics*; D010006:Osteoblasts / Q000502:physiology*; D016062:Porosity; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013499:Surface Properties; D014025:Titanium; D022161:Transport Vesicles / Q000235:genetics
D018797:Cell Cycle Proteins; D015921:Dental Implants; C009495:titanium dioxide; D014025:Titanium