Stability of polymorphic GC-rich repeat sequence-containing regions of Mycobacterium tuberculosis.
Mycobacterium tuberculosis cultures were subjected to DNA fingerprinting with IS6110- and polymorphic GC-rich sequence (PGRS)-containing probes. The PGRS banding patterns remained highly stable during multiple cultures of specimens from one disease episode (0.5% changed) and during transmission in patients with close contact (1.9% changed). Characteristic PGRS-restriction fragment length polymorphism motifs for different strain groupings may indicate distant evolutionary events leading to the differentiation of M. tuberculosis strain lineages.
MRC Centre for Molecular and Cellular Biology, Department of Medical Biochemistry, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa. mr6@sun.ac.za
Madalene
M
Richardson
Gian D
GD
van der Spuy
Samantha L
SL
Sampson
Nulda
N
Beyers
Paul D
PD
van Helden
Robin M
RM
Warren
D003596:Cytosine; D016172:DNA Fingerprinting; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D018900:Dinucleotide Repeats / Q000235:genetics*; D019143:Evolution, Molecular; D016680:Genome, Bacterial; D006147:Guanine; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D014376:Tuberculosis
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D006147:Guanine; D003596:Cytosine