Occurrence of the tdh and trh genes in Vibrio parahaemolyticus isolates from waters and raw shellfish collected in two French coastal areas and from seafood imported into France.
The occurrence of the hemolysin genes, tdh and trh, in Vibrio parahaemolyticus strains isolated from environmental samples collected in two French coastal areas, clinical samples, and seafood products imported into France was studied. Polymerase chain reaction (PCR) with two sets of primers was used to detect the hemolysin genes. Most of the clinical isolates (91%) and 1.5% of the isolates from seafood possessed the hemolysin genes. Three and fifteen percent, respectively, of the two groups of environmental strains carried the hemolysin genes depending on the geographic site. The tdh and trh genes play important roles in virulence. Thus, our results indicate that pathogenic V. parahaemolyticus isolates are present in French coastal areas and in seafood imported into France. Furthermore, they may also be present in French seafood products.
Robert-Pillot
Annick
A
Unité du Choléra et des Vibrions, Centre National de Référence des Vibrions et du Choléra, Institut Pasteur, 25, rue du Dr Roux, 75 724 Paris 15, France. arp@pasteur.fr
Guénolé
Alain
A
Lesne
Jean
J
Delesmont
Régis
R
Fournier
Jean-Michel
JM
Quilici
Marie-Laure
ML
D000818:Animals; D004783:Environmental Microbiology; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D005602:France; D006460:Hemolysin Proteins / Q000096:biosynthesis / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D017747:Seafood / Q000382:microbiology*; D012758:Shellfish / Q000382:microbiology*; D014736:Vibrio parahaemolyticus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
D006460:Hemolysin Proteins