Reconstructing chain functions in genetic networks.
Deciphering the mechanisms that control gene expression in the cell is a fundamental question in molecular biology. This task is complicated by the large number of possible regulation relations in the cell, and the relatively small amount of available experimental data. Recently, a new class of regulation functions called chain functions was suggested. Many signal transduction pathways can be accurately modeled by chain functions, and the restriction to chain functions greatly reduces the vast search space of regulation relations. In this paper we study the computational problem of reconstructing a chain function using a minimum number of experiments, in each of which only few genes are perturbed. We give optimal reconstruction schemes for several scenarios and show their application in reconstructing the regulation of galactose utilization in yeast.
Gat-Viks
I
I
School of Computer Science, Tel-Aviv University, Tel-Aviv 69978, Israel. iritg@tau.ac.il
Shamir
R
R
Karp
R M
RM
Sharan
R
R
D019295:Computational Biology; D005690:Galactose / Q000378:metabolism; D005786:Gene Expression Regulation; D008957:Models, Genetic; D015233:Models, Statistical; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
D012333:RNA, Messenger; D005690:Galactose