Protein NMR in biomedical research.
Nuclear magnetic resonance (NMR) spectroscopy is a versatile biophysical technique with wide applicability in drug discovery research, particularly for the detection and characterization of molecular interactions. This review highlights in a comprehensive manner the aspects of biomolecular NMR which are most beneficial for pharmaceutical research and presents them as contributions to the different stages of a drug discovery program: target selection, assay development, lead generation and lead optimization. Emphasis is put on the concept of the particular NMR application, rather than on technical details, and on recent examples. Finally, an appendix of frequently asked questions is given.
Novartis Institutes for BioMedical Research, Novartis Pharma AG, Lichtstrasse, 4002, Basel, Switzerland. wolfgang.jahnke@pharma.novartis.com.
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Jahnke
Novartis Institutes for BioMedical Research, Novartis Pharma AG, Lichtstrasse, 4002, Basel, Switzerland.
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Widmer
D000818:Animals; D002623:Chemistry Techniques, Analytical; D006801:Humans; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
D008024:Ligands; D011506:Proteins