Creating multiple time-shared laser traps with simultaneous displacement detection using digital signal processing hardware.
We present a design for implementing multiple laser traps for single-molecule studies through time-sharing using commercially available digital signal processing hardware in a computer running a standard multitasking operating system. This design enables four to six independent laser traps with a visitation frequency of 10,000s(-1)trap(-1) and a timing jitter of +/-0.5 micros to be created. The design also achieves nanometer-resolution detection of displacement in all of the traps simultaneously via back focal-plane interferometry and only a single quadrant photodiode detector. Practical design considerations and limitations together with the use of fiberlasers in laser traps are discussed. Using this device, the mechanokinetics of multiple molecular motors or adhesion proteins may be measured simultaneously. We present the example biological application of two kinesin-coated beads in separate traps moving on different portions of a microtubule.
Guilford
William H
WH
Department of Internal Medicine, Yale-New Haven Hospital, New Haven, CT 06510-3202, USA. guilford@virginia.edu
Tournas
Joshua A
JA
Dascalu
Dragos
D
Watson
Douglas S
DS
D004867:Equipment Design; D016547:Kinesins / Q000737:chemistry; D007834:Lasers; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods; D008863:Microspheres; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*; D012984:Software; D013997:Time Factors
D016547:Kinesins