Molecular analysis of Kaposi's sarcoma occurring during haemodialysis.
Human Herpesvirus 8 (HHV-8) has been implicated in the pathogenesis of Kaposi's sarcoma (KS). In this paper we attempted to confirm the connection between dialysis, HHV-8, and KS by examining the case of an elderly haemodialysis nonimmunosuppressed male patient with end-stage renal disease, who developed KS. By using PCR we have verified the presence of DNA from two different genomic regions (ORF 26 and ORF K1) of HHV-8. In addition, our RT-PCR results suggest active replication of HHV-8 in blood and KS lesions of the patient. Phylogenetic analysis revealed identical DNA sequence to ORF K1, and a close relation to its C1 variant. In conclusion, we document the case of KS and HHV-8 coexistence in a Greek elderly patient undergoing regular haemodialysis. Furthermore, our results indicate that factors other than immunosuppression could lead to KS development possibly due to activation of HHV-8.
Metaxa-Mariatou
V
V
Molecular Biology Research Centre HYGEIA Antonis Papayiannis, School of Medicine, University of Athens, Greece.
Chiras
T
T
Loli
A
A
Gazouli
M
M
Vallis
D
D
Nasioulas
G
G
D000368:Aged; D000369:Aged, 80 and over; D004279:DNA, Viral / Q000302:isolation & purification; D019288:Herpesvirus 8, Human / Q000302:isolation & purification*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000628:therapy*; D008297:Male; D006435:Renal Dialysis / Q000009:adverse effects*; D012514:Sarcoma, Kaposi / Q000821:virology*; D012878:Skin Neoplasms / Q000821:virology*; D019562:Viral Load
D004279:DNA, Viral