[Study on biology and epidemiological characteristics of vibrio cholerae non-O1 strains isolated from environmental waters in Foshan city].
OBJECTIVE
To study the distribution of serotype and the positive rate of toxins among vibrio cholerae non-O(1) isolated from environmental waters in Foshan city.


METHODS
Water specimens were collected from river and cultured for vibrio cholerae non-O(1). The PCR method was used to detect cholerae enterotoxin (CT) gene; the ELISA method was used to detect heat-stable toxin (ST) and heat-labile toxin (LT).


RESULTS
478 vibrio cholerae non-O(1) strains were isolated from 1 644 water specimens, with a positive rate of 29.07%. Serological assay showed that the main serotype of vibrio cholerae non-O(1) in Foshan city is VBO(7). Positive rate of CT, ST and LT were 1.91%, 13.14% and 12.17%, respectively.


CONCLUSIONS
A few non-O(1) strains were found to have several virulent factors simultaneously, and the results suggest that vibrio cholerae non-O(1) in environmental waters is potentially pathogenic and may affect people's health. It is necessary to pay attention to the prevention of diarrhoea caused by vibrio cholerae.
Chen
Ai-zhen
AZ
Center for Disease Control and Prevention of Foshan City, Guangdong Province, Foshan 528000, China.
Cai
Dai-song
DS
Zhu
Su-yi
SY
Huang
Zu-xing
ZX
Liao
Hua-le
HL
Yan
Guo-you
GY
Zhu
Wen
W
D002681:China; D004768:Enterotoxins / Q000235:genetics; D004784:Environmental Monitoring / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D016133:Polymerase Chain Reaction; D012621:Seasons; D012703:Serotyping; D044163:Vibrio cholerae non-O1 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014867:Water / Q000032:analysis*; D014871:Water Microbiology
D004768:Enterotoxins; C037420:stN protein, Vibrio cholerae; D014867:Water