[The studies on osteoblasts' mineralization and attachment affected by enamel matrix proteins].
OBJECTIVE
Investigate the effects of enamel matrix proteins(EMPs) on the mineralization and attachment of osteoblasts, to explore the possible mechanism of alveolar bone regeneration induced by enamel matrix proteins(EMPs).


METHODS
MC3T3-E1 osteoblasts were cultured in vitro and EMPs was added to medium in several concentrations. Cell's mineralizing ability was detected by Von-Kossa stain and attachment ability was detected by counting cells number.


RESULTS
The results manifested that EMPs promote more mineral nodules formation (P<0.01). But MC3T3-E1 cells' attachment ability was not improved (P>0.05).


CONCLUSIONS
EMPs enhanced the mineralizing function of osteoblasts, had no effects on implanting on root surface of osteoblasts, which may play an important role in the regeneration of alveolar bone.
Department of Oral Medicine, Ninth People's Hospital, School of Stomatology, Shanghai Second Medical University, Shanghai 200011, China. wuchunlan1000@sina.com.cn
Chun-lan
CL
Wu
Rong
R
Shu