Effects of paclitaxel on proliferation and apoptosis in human acute myeloid leukemia HL-60 cells.
OBJECTIVE
To investigate the regulatory effect of paclitaxel on proliferation and apoptosis in human acute leukemia HL-60 cells.


METHODS
HL-60 cell growth was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tertrazolium bromide (MTT) colorimetric assay. Cell cycle kinetics and apoptosis were analyzed by flow cytometry and microscopic examination. In addition, DNA microarrays containing 14,400 EST elements were used to investigate the gene expression pattern of HL-60 cells exposed to paclitaxel 1 micromol/L.


RESULTS
Paclitaxel inhibited HL-60 cell growth significantly in a dose-dependent and time-dependent manner (P<0.01). Marked cell accumulation in G2/M phase and multinucleated cells were also observed after treatment with paclitaxel 0.1 and 1 micromol/L. Among 14400 EST elements, 277 genes were found to be markedly up- or down-expressed in the HL-60 cells treated with paclitaxel 1 micromol/L for 0.5 h, comprising 210 known genes and 67 unknown genes.


CONCLUSIONS
Paclitaxel suppresses the growth of HL-60 cells in vitro by causing cell-cycle arrest and apoptosis. The results of microarray suggest that paclitaxel initiates apoptosis through multiple mechanisms.
Wan
Yun-feng
YF
Department of Pharmacology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Guo
Xue-qing
XQ
Wang
Zheng-hua
ZH
Ying
Kang
K
Yao
Ming-hui
MH
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D020869:Gene Expression Profiling; D018922:HL-60 Cells; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D017239:Paclitaxel / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D017239:Paclitaxel