Activation of carbonic anhydrase II by active-site incorporation of histidine analogs.
The hydration of CO2 catalyzed by human carbonic anhydrase II (HCA II) is accompanied by proton transfer from the zinc-bound water of the enzyme to solution. We have replaced the proton shuttling residue His 64 with Ala and placed cysteine residues within the active-site cavity by mutating sites Trp 5, Asn 62, Ile 91, and Phe 131. These mutants were modified at the single inserted cysteine with imidazole analogs to introduce new potential shuttle groups. Catalysis by these modified mutants was determined by stopped-flow and 18O-exchange methods. Specificity in proton transfer was demonstrated; only modifications of the Cys 131-containing mutant showed enhancement in the proton transfer step of catalysis compared with unmodified Cys 131-containing mutant. Modifications at other sites resulted in up to 3-fold enhancement in rates of CO2 hydration, with apparent second-order rate constants near 350 microM(-1) s(-1). These are among the largest values of kcat/Km observed for a carbonic anhydrase.
Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, FL 32610-0267, USA.
Ileana
I
Elder
Shoufa
S
Han
Chingkuang
C
Tu
Heather
H
Steele
Philip J
PJ
Laipis
Ronald E
RE
Viola
David N
DN
Silverman
D001665:Binding Sites; D002245:Carbon Dioxide / Q000378:metabolism; D024402:Carbonic Anhydrase II / Q000235:genetics / Q000378:metabolism*; D006639:Histidine / Q000031:analogs & derivatives*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009154:Mutation; D017434:Protein Structure, Tertiary; D011522:Protons
D011522:Protons; D002245:Carbon Dioxide; D006639:Histidine; D024402:Carbonic Anhydrase II