[Determination of the structure of the main component of an antibiotic complex produced by Streptomyces griseolus 182].
In the programme for screening antibiotics with antifungal and immunosuppressing activity a culture of Streptomyces griseolus 182 was isolated. The culture produced a complex of oligomycin structure antibiotics. Individual components of the complex were isolated by HPLC. The complex was shown to contain three components at a ratio of 80:15:5. The findings were compared with the physico-chemical characteristics of the described antibiotics. On the basis of the analysis it was concluded that fraction 2 of the antibiotic complex 182 could be oligomycin A. A detailed comparative investigation of oligomycin A and component 2 with HPLC, FMR, IR spectroscopy and mass spectrometry revealed their identity. The other two components were shown to be oligomycins B and C.
Kabanov
A E
AE
I. M. Sechenov Moscow Medical Academy, N. M. Emmanuel Research Institute of Biochemical Physics, Russian Academy of Sciences, National Research Centre of Antibiotics, Moscow.
Danilenko
A N
AN
Spiridonova
I A
IA
Bibikova
M V
MV
Grammatikova
N E
NE
Orekhov
S N
SN
Katlinskiĭ
A V
AV
D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D009840:Oligomycins / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013302:Streptomyces / Q000378:metabolism*
D009840:Oligomycins; C031004:oligomycin A; C048335:oligomycin B; C042075:oligomycin C