[Effects of clinorotation-induced weightlessness on growth factor and ERK signal transduction in cultured cardiac fibroblasts].
OBJECTIVE
To study the changes of expression and secretion of some growth factors and ERK1/2 activation in cardiac fibroblasts under clinorotation conditions.


METHODS
Primary cultured neonatal rat cardiac fibroblasts were clinorotated to simulate weightlessness. The bFGF and TGF(beta)1 expressions in the cells and Ang II concentration in the medium were detected by western blotting and radioimmunoassay respectively. The expression and phosphorylated level of ERK1/2 were analyzed by western blotting.


RESULTS
Compared to the control group, bFGF expression and Ang II concentration increased in clinorotation group, but there was no evident change in TGF(beta)1 expression. At the same time, the expression of ERK1/2 increased but their phosphorylated level decreased.


CONCLUSIONS
Different growth factors responds to clinorotation by different ways. As one of the most important reactions of growth factor signal downstream pathways, ERK1/2 activation is inhibited in clinorotation.
Institute of Space Medico-Engineering, Beijing, China.
Fen
F
Yang
Ying-Hui
YH
Li
Yong-Jie
YJ
Ma
Ping
P
Zhong
Jin-ping
JP
Song
Zhong-quan
ZQ
Dai
D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D006112:Gravitation; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D012399:Rotation; D016212:Transforming Growth Factor beta / Q000378:metabolism; D018474:Weightlessness Simulation
D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; D000804:Angiotensin II; D048049:Extracellular Signal-Regulated MAP Kinases