Effect of RG1192, a substituted dextran, on inflammatory response.
RG1192 is one of a series of substituted dextran heparin-like polymers that were shown to accelerate tissue repair in various experimental models. Many efforts have been made to investigate the mechanisms by which these compounds act In these experiments, we studied the effect of RG1192 on inflammatory cells. We showed that intraperitoneal RG1192 injection caused a 5-fold increase in peritoneal polymorphonuclear (PMN) cell number at 6 hr, 8-fold increase at 12 hr and 3-fold increase at 24 hr, as compared to controls. Although the circulating white blood cell count did not show any changes, the number of circulating PMN showed a small early increase. Further investigation had been done on the chemotactic effect of RG1192 on PMN in vitro. The results showed that RG1192 alone increased PMN migration at 0.5 mg/ml and 1 mg/ml, without potentiation by additional IL-8.
Laboratoire de Recherche sur la Croissance Cellulaire, la Régénération et la Reparation Tissulaires, CRRET, ESA CNRS 70-53, Université Paris 12, Avenue du Général de Gaulle, 94000 Créteil, France.
P
P
Li
J
J
Cebrian
G
G
Carpentier
I
I
Barbosa
D
D
Barritault
D000818:Animals; D002633:Chemotaxis / Q000187:drug effects; D003911:Dextrans / Q000737:chemistry / Q000494:pharmacology*; D007249:Inflammation / Q000201:enzymology / Q000276:immunology* / Q000473:pathology; D007958:Leukocyte Count; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D003911:Dextrans; C475611:RG1192; D009195:Peroxidase