Effects of triclosan on the early life stages and reproduction of medaka Oryzias latipes and induction of hepatic vitellogenin.
Triclosan (2,4,4'-trichloro-2'-hydroxydiphenyl ether) is widely used as antibacterial agent in various industrial products, such as textile goods, soap, shampoo, liquid toothpaste and cosmetics, and often detected in wastewater effluent. In this study, the effects of TCS on the early life stages and reproduction of medaka (Oryzias latipes) were investigated. The 96-h median lethal concentration value of TCS for 24-h-old larvae was 602 microg/l. The hatchability and time to hatching in fertilized eggs exposed to 313 microg/l TCS for 14 days were significantly decreased and delayed, respectively. An assessment of the effects of a TCS 21-day exposure period on the reproduction of paired medaka showed no significant differences in the number of eggs produced and fertility among the control and 20, 100 and 200 microg/l TCS treatment groups. However, concentrations of hepatic vitellogenin were increased significantly in males treated with TCS at 20 and 100 microg/l. In the F(1) generations, although the hatching of embryos in the 20 microg/l treatment showed adverse effects, there was no dose-response relationship between hatchability and TCS treatment levels. These results suggest that TCS has high toxicity on the early life stages of medaka, and that the metabolite of TCS may be a weak estrogenic compound with the potential to induce vitellogenin in male medaka but with no adverse effect on reproductive success and offspring.
Ishibashi
Hiroshi
H
Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, 3-1-100 Tsukide, Kumamoto 862-8502, Japan.
Matsumura
Naomi
N
Hirano
Masashi
M
Matsuoka
Munekazu
M
Shiratsuchi
Hideki
H
Ishibashi
Yasuhiro
Y
Takao
Yuji
Y
Arizono
Koji
K
D000818:Animals; D001837:Body Weights and Measures; D008099:Liver / Q000378:metabolism; D009990:Oryzias / Q000196:embryology* / Q000502:physiology*; D012098:Reproduction / Q000187:drug effects* / Q000502:physiology; D018675:Toxicity Tests; D014260:Triclosan / Q000633:toxicity*; D014818:Vitellogenesis / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D014874:Water Pollutants, Chemical; D014260:Triclosan