Characterization of the various forms of the Reelin protein in the cerebrospinal fluid of normal subjects and in neurological diseases.
Reelin is a large extracellular glycoprotein that is defective in reeler mutant mice and plays a well-established role during brain development in human as well as rodents. In the adult brain, Reelin is expressed in a subset of GABAergic interneurons. Its role in disease states is not clearly defined, although it is implicated in autism and psychoses such as schizophrenia. In this report, we show that Reelin immunoreactive proteins can be detected in the human cerebrospinal fluid (CSF) with monoclonal antibodies directed against the N- and C-terminal regions of the protein. In CSF, Reelin is present as different products due to processing at two main sites; preservation at -20 degrees C increases processing further. CSF Reelin originates from the brain tissue and not from plasma. The protein was detected in comparable concentrations in children and adults, and the signal varied largely from subject to subject with no obvious correlation with age or neurological disease state.
Ignatova
Nina
N
Developmental Genetics Unit, University of Louvain Medical School, B1200 Brussels, Belgium.
Sindic
Christian J M
CJ
Goffinet
André M
AM
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology; D001921:Brain / Q000276:immunology / Q000378:metabolism* / Q000503:physiopathology; D015816:Cell Adhesion Molecules, Neuronal / Q000134:cerebrospinal fluid* / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D018450:Disease Progression; D016326:Extracellular Matrix Proteins / Q000134:cerebrospinal fluid* / Q000276:immunology; D006801:Humans; D007223:Infant; D008875:Middle Aged; D009419:Nerve Tissue Proteins; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid* / Q000276:immunology / Q000503:physiopathology; D017434:Protein Structure, Tertiary / Q000502:physiology; D000091042:Reelin Protein; D012016:Reference Values; D012697:Serine Endopeptidases
D000911:Antibodies, Monoclonal; D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D000091042:Reelin Protein; C000717127:RELN protein, human; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases