Optical sectioning using a fiber probe with an angled illumination-collection geometry: evaluation in engineered tissue phantoms.
We present a fiber optic probe that combines polarized illumination and detection with an angled distal probe geometry to detect the size-dependent scattering at a specific depth within epithelium. Analysis of the scattering signal by use of Mie theory allows the extraction of scatterer size and size distribution-key parameters for precancer detection. The probe was evaluated in two tissue phantoms: polystyrene beads atop collagen gel and multiple layers of cancer cells atop collagen. We also present in vivo measurements in the oral cavity of normal volunteers. The sizes of scatterers extracted from the scattering spectra corresponded to independently measured values.
Department of Physics, The University of Texas at Austin, Austin, Texas 78712, USA.
Linda
L
Nieman
Alexey
A
Myakov
Jesse
J
Aaron
Konstantin
K
Sokolov
D001698:Biomedical Engineering; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000648:ultrastructure*; D004867:Equipment Design; D005260:Female; D005336:Fiber Optic Technology / Q000295:instrumentation*; D006801:Humans; D008863:Microspheres; D008962:Models, Theoretical; D009061:Mouth Mucosa / Q000648:ultrastructure; D055100:Optical Fibers; D019047:Phantoms, Imaging; D011137:Polystyrenes; D012542:Scattering, Radiation; D002583:Uterine Cervical Neoplasms / Q000648:ultrastructure
D011137:Polystyrenes