Abnormal expression of tyrosine hydroxylase not accompanied by phosphorylation at serine 40 in cerebellar Purkinje cells of ataxic mutant mice, rolling mouse Nagoya and dilute-lethal.
This study examined immunohistochemically the expression of an enzymatically active form of tyrosine hydroxylase (TH), phosphorylated TH at Ser40 (phospho-TH), in the cerebellum of ataxic mutant mice, rolling mouse Nagoya (RMN) and dilute-lethal (DL). TH immunostaining appeared in some Purkinje cells in RMN and DL, but in a few of the Purkinje cells of littermate controls for both mutants. In all groups of mice, there were no phospho-TH immunoreactive Purkinje cells in the cerebellum, although the subsets of TH immunoreactive Purkinje cells were found in the adjacent sections. The results suggest that TH expression in the Purkinje cells of ataxic mutants abnormally increases without activation of this enzyme by phosphorylation. This may mean that TH in Purkinje cells is not related to catecholamine synthesis.
Sawada
Kazuhiko
K
Department of Anatomy and Developmental Neurobiology, University of Tokushima School of Medicine, Japan. sawada@basic.med.tokushima-u.ac.jp
Ando
Masahiro
M
Sakata-Haga
Hiromi
H
Sun
Xue-Zhi
XZ
Jeong
Young-Gil
YG
Hisano
Setsuji
S
Takeda
Noriaki
N
Fukui
Yoshihiro
Y
D000818:Animals; D001259:Ataxia / Q000235:genetics* / Q000378:metabolism; D002531:Cerebellum / Q000473:pathology; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D010766:Phosphorylation; D011689:Purkinje Cells / Q000378:metabolism*; D012694:Serine / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics* / Q000378:metabolism
D012694:Serine; D014446:Tyrosine 3-Monooxygenase