Dehydrotrametenonic acid and dehydroeburiconic acid from Poria cocos and their inhibitory effects on eukaryotic DNA polymerase alpha and beta.
A new lanostane-type triterpene acid, (20xi)-3-oxolanosta-7,9(11),24-trien-21-oic acid (1; dehydrotrametenonic acid), along with a known triterpene acid, dehydroeburiconic acid (2), were isolated from the epidermis of the sclerotia of Poria cocos. The structure of 1 was analyzed on the basis of spectroscopic methods. Compounds 1 and 2 inhibited calf DNA polymerase alpha and rat DNA polymerase beta, with the 50% inhibition values of 45.5 microM (1) and 40.8 microM (2), and 86.5 microM (1) and 30.0 microM (2), respectively.
Akihisa
Toshihiro
T
College of Science and Technology, Nihon University, Tokyo, Japan. akihisa@chem.cst.nihon-u.ac.jp
Mizushina
Yoshiyuki
Y
Ukiya
Motohiko
M
Oshikubo
Manabu
M
Kondo
Seizo
S
Kimura
Yumiko
Y
Suzuki
Takashi
T
Tai
Takaaki
T
D000818:Animals; D002417:Cattle; D004256:DNA Polymerase I / Q000037:antagonists & inhibitors*; D019951:DNA Polymerase beta / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008970:Molecular Weight; D020072:Polyporales / Q000737:chemistry*; D051381:Rats; D014315:Triterpenes / Q000138:chemical synthesis* / Q000302:isolation & purification* / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D014315:Triterpenes; C492580:dehydroeburiconic acid; C487641:dehydrotrametenonic acid; D004256:DNA Polymerase I; D019951:DNA Polymerase beta