Analysis of digenic epistatic effects and QE interaction effects QTL controlling grain weight in rice.
Immortalized F(2) population of rice (Oryza sativa L.) was developed by randomly mating F(1) among recombinant inbred (RI) lines derived from (Zhenshan 97B x Minghui 63), which allowed replications within and across environments. QTL (quantitative trait loci) mapping analysis on kilo-grain weight of immortalized F(2) population was performed by using newly developed software for QTL mapping, QTLMapper 2.0. Eleven distinctly digenic epistatic loci included a total of 15 QTL were located on eight chromosomes. QTL main effects of additive, dominance, and additive x additive, additive x dominance, and dominance x dominance interactions were estimated. Interaction effects between QTL main effects and environments (QE) were predicted. Less than 40% of single effects, most of which were additive effects, for identified QTL were significant at 5% level. The directional difference for QTL main effects suggested that these QTL were distributed in parents in the repulsion phase. This should make it feasible to improve kilo-grain weight of both parents by selecting appropriate new recombinants. Only few of the QE interaction effects were significant. Application prospect for QTL mapping achievements in genetic breeding was discussed.
Agronomy Department, Zhejiang University, Hangzhou 310029, China.
Yong-ming
YM
Gao
Jun
J
Zhu
You-shen
YS
Song
Ci-xin
CX
He
Chun-hai
CH
Shi
Yong-zhong
YZ
Xing
D032461:Chromosomes, Plant / Q000235:genetics; D003433:Crosses, Genetic; D004777:Environment; D004843:Epistasis, Genetic; D012275:Oryza / Q000033:anatomy & histology* / Q000235:genetics*; D010641:Phenotype; D040641:Quantitative Trait Loci