Animal deoxyribonucleoside kinases: 'forward' and 'retrograde' evolution of their substrate specificity.
Deoxyribonucleoside kinases, which catalyse the phosphorylation of deoxyribonucleosides, are present in several copies in most multicellular organisms and therefore represent an excellent model to study gene duplication and specialisation of the duplicated copies through partitioning of substrate specificity. Recent studies suggest that in the animal lineage one of the progenitor kinases, the so-called dCK/dGK/TK2-like gene, was duplicated prior to separation of the insect and mammalian lineages. Thereafter, insects lost all but one kinase, dNK (EC 2.7.1.145), which subsequently, through remodelling of a limited number of amino acid residues, gained a broad substrate specificity.
BioCentrum-DTU, Eukaryote Molecular Biology Group, Building 301, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark. jp@biocentrum.dtu.dk
Jure
J
Piskur
Michael P B
MP
Sandrini
Wolfgang
W
Knecht
Birgitte
B
Munch-Petersen
D000595:Amino Acid Sequence; D000818:Animals; D017124:Conserved Sequence; D004247:DNA / Q000737:chemistry; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D009694:Nucleic Acid Precursors / Q000737:chemistry; D010766:Phosphorylation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D017354:Point Mutation; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D009694:Nucleic Acid Precursors; D004247:DNA; D017853:Phosphotransferases (Alcohol Group Acceptor); C020018:deoxyribonucleoside kinases