Regulation of feeding by galnon.
Galanin is a neuropeptide that has been implicated in multiple bioactivities, inter alia eating disorders. In this study, we have examined the effects of galnon, a novel low molecular weight galanin receptor ligand. Previous studies have shown that galnon acts as a systemically active, blood-brain barrier crossing agonist on galanin signaling both in vitro and in vivo, inhibiting pentylenetetrazole-induced seizures. Here, intracerebroventricular (10-20 microg) and intraperitoneal (1.5-5 mg/kg) administration of galnon induced a strong, dose-dependent reduction of food intake in rats and mice. This reduction in feeding occurred without reducing general activity and was shown to be attenuated by an intracerebroventricular administration of M35, a peptide galanin antagonist. These data demonstrate that galnon is a promising tool for studies of the involvement of galanin in feeding disorders and other behavioral processes.
Department of Physiology, Tartu University, Ravila 19, Tartu 50 411, Estonia.
Urho
U
Abramov
Anders
A
Florén
David J
DJ
Echevarria
Ariel
A
Brewer
Honeyleen
H
Manuzon
John K
JK
Robinson
Tamas
T
Bartfai
Eero
E
Vasar
Ulo
U
Langel
D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D001920:Bradykinin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D003374:Coumarins / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000187:drug effects*; D019004:Galanin / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D007274:Injections, Intraperitoneal; D007276:Injections, Intraventricular; D008024:Ligands; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity / Q000187:drug effects; D010446:Peptide Fragments / Q000008:administration & dosage / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D044088:Receptors, Galanin / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects
C456547:7-((9-fluorenylmethoxycarbonyl)cyclohexylalanyllysyl)amino-4-methylcoumarin; D003374:Coumarins; D008024:Ligands; D010446:Peptide Fragments; D044088:Receptors, Galanin; C074069:galanin-(1-13)-bradykinin-(2-9)-amide; D019004:Galanin; D001920:Bradykinin