Identification of glutamate transporters and receptors in mouse testis.
OBJECTIVE
To investigate the presence of glutamate transporters and receptors in mouse testis.


METHODS
Glutamate uptake analysis was performed to study the function of glutamate transporters in mouse testis. Comparative RT-PCR technique and sequencing analysis were used to study the expression of glutamate receptors and transporters in mouse testis.


RESULTS
Mouse testis possessed glutamate uptake capacity with sodium-dependence. Vmax value of glutamate uptake was (1.60 +/- 0.21) pmol/min per mg protein and Km value of glutamate uptake was (11.0+/-1.6) micromol/L in mouse testis according to saturation analysis. Furthermore, the uptake activity could be inhibited by DHK (GLT1 selective inhibitor) and THA (glutamate uptake inhibitor). In addition, RT-PCR results revealed that glutamate transporters (GLT1 and EAAC1) and ionotropic glutamate receptors (NR1, NR2B, GluR6 and KA2) were expressed in mouse testis.


CONCLUSIONS
Glutamate transporters and receptors do exist in mouse testis.
Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Jia-hua
JH
Hu
Na
N
Yang
Ying-hua
YH
Ma
Jie
J
Jiang
Jin-fu
JF
Zhang
Jian
J
Fei
Li-he
LH
Guo
D027322:Amino Acid Transport System X-AG / Q000378:metabolism; D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D001921:Brain / Q000166:cytology; D002478:Cells, Cultured; D027342:Excitatory Amino Acid Transporter 2 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D050580:Excitatory Amino Acid Transporter 3; D050491:Glutamate Plasma Membrane Transport Proteins; D018698:Glutamic Acid / Q000493:pharmacokinetics*; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018092:Kainic Acid Receptors / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D027981:Symporters / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D000097806:GluK2 Kainate Receptor
D027322:Amino Acid Transport System X-AG; D001224:Aspartic Acid; D027342:Excitatory Amino Acid Transporter 2; D050580:Excitatory Amino Acid Transporter 3; D050491:Glutamate Plasma Membrane Transport Proteins; D018698:Glutamic Acid; D007608:Kainic Acid; D018092:Kainic Acid Receptors; D016194:Receptors, N-Methyl-D-Aspartate; D027981:Symporters; D000097806:GluK2 Kainate Receptor; C409466:NR1 NMDA receptor; C121001:NR2B NMDA receptor; C493399:Slc1a1 protein, mouse; C011274:3-hydroxyaspartic acid; C020889:dihydrokainic acid