Laminin-1 and SIKVAV a laminin-1-derived peptide, regulate the morphology and protease activity of a human salivary gland adenoid cystic carcinoma cell line.
In a previous paper, we demonstrated that laminin-1 and its derived peptide SIKVAV modulates the morphology of an adenoid cystic carcinoma cell line (CAC2 cells). Light microscopy of CAC2 cells grown in three-dimensional preparations of SIKVAV-enriched laminin-1 showed the presence of pseudocystic spaces. Pseudocysts are hallmarks of adenoid cystic carcinoma in vivo. We hypothesized that these pseudocystic spaces could be due to the protease-inducing/activating role of SIKVAV. Thus, we studied the presence of matrix metalloproteinases (MMPs) in CAC2 cells treated either by laminin-1 or by SIKVAV-enriched laminin-1. Immunohistochemistry and zymography suggested that SIKVAV enhanced the secretion of MMP-2 and MMP-9 in CAC2 cells. We propose that SIKVAV induces pseudocystic formation probably through the secretion of MMPs 2 and 9.
Department of Histology and Embryology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 1524, São Paulo SP 05508-900, Brazil.
V M
VM
Freitas
B
B
Scheremeta
M P
MP
Hoffman
R G
RG
Jaeger
D003528:Carcinoma, Adenoid Cystic / Q000201:enzymology* / Q000473:pathology; D045744:Cell Line, Tumor / Q000187:drug effects; D003470:Culture Media; D003593:Cytoplasm / Q000737:chemistry; D005110:Extracellular Space / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007797:Laminin / Q000378:metabolism / Q000494:pharmacology*; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D020782:Matrix Metalloproteinases / Q000032:analysis*; D009842:Oligopeptides / Q000378:metabolism / Q000494:pharmacology*; D012468:Salivary Gland Neoplasms / Q000201:enzymology* / Q000473:pathology
D003470:Culture Media; D007797:Laminin; D009842:Oligopeptides; C121346:laminin 1; C077583:seryl-isoleucyl-lysyl-valyl-alanyl-valinamide; D020782:Matrix Metalloproteinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9