Phosphorylation of translation initiation factor eIF2alpha in the brain during pilocarpine-induced status epilepticus in mice.
In this work, we show extensive phosphorylation of the alpha subunit of translation initiation factor 2 (eIF2alpha) occurring in the brain of mice subjected to 30 min of status epilepticus induced by pilocarpine. eIF2alpha(P) immunoreactivity was detected in the hippocampal pyramidal layer CA1 and CA3, cortex layer V, thalamus and amygdala. After 2 h of recovery, there was a marked decrease in total brain eIF2alpha(P), with the cortex layer V showing the most pronounced loss of anti-eIF2alpha(P) labeling, whereas the CA1 subregion had a significant increase in eIF2alpha(P). These results indicate that inhibition of protein synthesis in experimental models of epilepsy might be due to low levels of eIF2-GTP caused by the phosphorylation of eIF2alpha, and suggest that translational control may contribute to cell fate in the affected areas.
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, Rua Botucatu 862, 5 andar, São Paulo, SP 04023-062, Brazil.
Larissa S
LS
Carnevalli
Cátia M
CM
Pereira
Beatriz M
BM
Longo
Carolina B
CB
Jaqueta
Marcelo
M
Avedissian
Luiz Eugênio A M
LE
Mello
Beatriz A
BA
Castilho
D000818:Animals; D015153:Blotting, Western / Q000379:methods; D001921:Brain / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D003720:Densitometry / Q000379:methods; D004195:Disease Models, Animal; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D051379:Mice; D010766:Phosphorylation / Q000187:drug effects; D010862:Pilocarpine; D013226:Status Epilepticus / Q000139:chemically induced / Q000378:metabolism*; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism*
D014157:Transcription Factors; D010862:Pilocarpine; C101003:ELF2 protein, human