DNA sequencing and analysis of the right-hand part of the genome of the unique bovine adenovirus type 10.
The prototype strain of bovine adenovirus (BAdV) type 10 and four additional isolates that were indistinguishable in serum-neutralization tests have been shown to have remarkable variation in their genome size and restriction maps. In the present study, more than 40 % of the DNA sequence of the BAdV-10 isolate with the longest genome was determined. A biased base composition resulting in low (<41 %) GC content was noticed. Analysis of the genes of the DNA-binding protein, 100K, 33K, pVIII and fibre proteins, as well as early regions E3 and E4, which are encoded by the genome fragment examined, confirmed that BAdV-10 is different from the other known BAdV types regarding its phylogenetic distance and the organization of its exceptionally short E3 region, apparently containing only two genes. A comparative analysis of the E3 and E4 regions of BAdV-10 with various animal adenoviruses revealed interesting features accounting for the very short genome of BAdV-10. In the examined BAdV-10 isolate, duplicated sequences were localized in and around the fibre gene. Since BAdV-10 appears to be pathogenic to cattle and is genetically distant from the other BAdVs, we suggest that BAdV-10 is not a genuine bovine virus, but has recently switched host and is now undergoing an adaptation process in its new host. In accordance with this hypothesis, the remarkable predominance of AT-rich codons along with the variable fibre gene might be signs of adaptation.
Ursu
Krisztina
K
Central Veterinary Institute, PO Box 2, H-1581 Budapest, Hungary.|Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, H-1581 Budapest, Hungary.
Harrach
Balázs
B
Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, H-1581 Budapest, Hungary.
Matiz
Katalin
K
Veterinary Institute of Debrecen, PO Box 51, H-4002 Debrecen, Hungary.|Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, H-1581 Budapest, Hungary.
Benkő
Mária
M
Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, H-1581 Budapest, Hungary.
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D016679:Genome, Viral; D017921:Mastadenovirus / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D004279:DNA, Viral; D014764:Viral Proteins