Protection against brucellosis in goats, five years after vaccination with reduced-dose Brucella melitensis Rev 1 vaccine.
The protection conferred by the reduced-dose Rev 1 Brucella melitensis vaccine in goats that had been immunized 5 years previously was evaluated. Sixteen goats vaccinated 5 years before with Rev 1 (1 x 10(5) cfu) and 5 non-vaccinated goats were challenged with B. melitensis 16M (4 x 10(5) cfu) using the conjunctival route. After giving birth or aborting, the goats were sacrificed and tissue samples were taken for bacteriological study. The challenge strain was recovered in 12%, of the animals from the vaccinated group, and in (80% of the control group. It is concluded, therefore that the use of reduced-dose Rev 1 protects goats vaccinated in endemic areas for at least 5 years after immunization.
Díaz-Aparicio
E
E
CENID Microbiología, Instituto Nacional de Investigaciones Agrícolas, Forestales y Pecuarias, Carretera Federal México-Toluca Km. 15.5, Cuajimalpa, Mexico. diaz@ciml.univ-mrs.fr
Hernández
L
L
Suárez-Güemes
F
F
D000818:Animals; D002004:Brucella Vaccine / Q000008:administration & dosage* / Q000276:immunology; D017347:Brucella melitensis / Q000276:immunology*; D002006:Brucellosis / Q000517:prevention & control / Q000662:veterinary*; D015169:Colony Count, Microbial / Q000662:veterinary; D003228:Conjunctiva; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D015511:Goat Diseases / Q000517:prevention & control*; D006041:Goats; D013997:Time Factors
D002004:Brucella Vaccine