Studies on acute human infections using FTIR microspectroscopy and cluster analysis.
A novel methodology for the diagnosis of acute infections using FTIR microspectroscopy (FTIR-MSP) data on blood components and cluster analysis is presented. Blood samples were collected from 11 patients suffering from various infections and 16 age-matched healthy human controls. Blood components such as white blood cells, red blood cells, and plasma were isolated using standard procedures and FTIR-MSP of these components was utilized. A cluster analysis of the FTIR spectra was performed. The spectra obtained from the three blood components of patients were different from those of controls. The FTIR spectra of white blood cells from patients suffering infections were significantly different from the controls. Cluster analyses of averaged FTIR-MSP spectra of white blood cells provided 100% classification between patients and healthy controls.
Department of Pediatric Surgery, Soroka University Medical Center, Ben Gurion University, Beer Sheva 84101, Israel. shaulm@bgumail.bgu.ac.il
Jacov
J
Mordehai
Jagannathan
J
Ramesh
Mahmoud
M
Huleihel
Zahavi
Z
Cohen
Oleg
O
Kleiner
Marina
M
Talyshinsky
Vitaly
V
Erukhimovitch
Aviv
A
Cahana
Ahmad
A
Salman
Ranjit Kumar
RK
Sahu
Hugo
H
Guterman
Shaul
S
Mordechai
D000208:Acute Disease; D016022:Case-Control Studies; D002648:Child; D016000:Cluster Analysis; D004912:Erythrocytes / Q000737:chemistry; D006801:Humans; D007239:Infections / Q000097:blood / Q000175:diagnosis; D007557:Israel; D007962:Leukocytes / Q000737:chemistry; D015591:Microspectrophotometry; D010949:Plasma / Q000737:chemistry; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared