Enhancement of tanshinone production in Salvia miltiorrhiza hairy root culture by Ag+ elicitation and nutrient feeding.
Ag (+) was employed as an abiotic elicitor to stimulate the secondary metabolite production in hairy root culture of Salvia miltiorrhiza, a valuable herbal plant. The addition of Ag (2)S (2)O (3) at 15 - 40 microM to the culture between 12 and 22 days post inoculation resulted in more than 2-fold increase in the yields of the three diterpenoid tanshinones, tanshinone I, tanshinone IIA and cryptotanshinone. This stimulating effect was dependent on the Ag (+) dose, the day of Ag (+) addition to the culture and the tanshinone species, while the total yield of the three tanshinones (TT) was mainly dependent on the Ag (+) dose, with the highest being attained at 30 microM. The Ag (+) treatment also caused a dose-dependent inhibition of hairy root growth. Sucrose feeding or medium renewal before the addition of Ag (+) to the culture effectively prevented the growth inhibition, and significantly increased the biomass concentration and volumetric tanshinone yield. With combined medium renewal and Ag (+) treatment, in particular, the TT yield was increased 6.6-fold relative to that of the control (55.7 mg/L versus 7.3 mg/L). The elicitor function of Ag (+) in the hairy root culture was validated by its ability to induce the characteristic elicitor responses of plants, the increase in cross-cell membrane ion fluxes and the production of reactive oxygen species.
Department of Applied Biology & Chemical Technology, the Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong. bcjywu@polyu.edu.hk
Changhe
C
Zhang
Qiong
Q
Yan
Wai-Keung
WK
Cheuk
Jianyong
J
Wu
D045784:Abietanes; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000378:metabolism*; D006801:Humans; D010616:Phenanthrenes / Q000378:metabolism*; D008517:Phytotherapy; D018517:Plant Roots / Q000378:metabolism; D027561:Salvia miltiorrhiza / Q000378:metabolism*; D013395:Sucrose; D013885:Thiosulfates / Q000008:administration & dosage / Q000494:pharmacology*
D045784:Abietanes; D004365:Drugs, Chinese Herbal; D010616:Phenanthrenes; D013885:Thiosulfates; C021751:tanshinone; C095213:silver thiosulfate; D013395:Sucrose