A new method for recombinant adeno-associated virus vector delivery to murine diaphragm.
Genetically modified mice are important models for evaluation of potential gene therapies for human diseases. However, their small size often precludes the use of clinically feasible methods for vector delivery, therefore, alternative methods must be used. We have developed a gel-based method for delivery of recombinant adeno-associated virus vectors to the mouse diaphragm, an important target organ for many myopathic diseases. We hypothesized that delivery of vectors in a viscous solution would increase transduction by providing a longer exposure time to target cells. We demonstrate that gel-mediated delivery of rAAV serotypes 1, 2, and 5 results in higher transduction efficiencies than free vectors alone when administered in vivo to mouse diaphragms. We further establish greater tropism of rAAV1 vectors for the diaphragm compared to serotypes 2 and 5. This report describes a novel method for efficient delivery of rAAV vectors to the mouse diaphragm and is the first demonstration of gene transfer to the diaphragm using recombinant adeno-associated virus vectors.
Powell Gene Therapy Center, University of Florida, Gainesville, 32610, USA. cmah@ufl.edu
Cathryn
C
Mah
Thomas J
TJ
Fraites
Kerry O
KO
Cresawn
Irene
I
Zolotukhin
Melissa A
MA
Lewis
Barry J
BJ
Byrne
D000818:Animals; D000229:Dependovirus / Q000235:genetics*; D003964:Diaphragm / Q000378:metabolism*; D004195:Disease Models, Animal; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D016679:Genome, Viral; D005959:Glucosidases / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D011994:Recombinant Proteins; D006003:Glycogen; D005959:Glucosidases; D001616:beta-Galactosidase