Comparison of preparative HPLC/MS and preparative SFC techniques for the high-throughput purification of compound libraries.
A diverse set of 16 high-throughput organic synthesis libraries, consisting of 48 samples per library, has been purified by both preparative supercritical fluid chromatography (SFC) and preparative high-performance liquid chromatography (HPLC). This paper details the relative effectiveness of these two purification techniques in terms of success, yield, and purity of final product.
Searle
Philip A
PA
Medicinal Chemistry Technologies, Global Pharmaceutical Research & Development, Abbott Laboratories, Department R4CP, Building AP10, 100 Abbott Park Road, Abbott Park, IL 60064-6101, USA. philip.searle@abbott.com
Glass
Keithney A
KA
Hochlowski
Jill E
JE