[The neurotrophic effect of endogenous NT-3 from adult cat spared dorsal root ganglion on ganglionic neurons].
OBJECTIVE
To investigate the neurotrophic effect of endogenous NT-3 from adult cat dorsal root ganglion (DRG) on ganglionic neurons.


METHODS
Rhizotomy of bilateral L1, L3, L5 and L7 dorsal roots of cats was performed, leaving L2, L4 and L6 DRG as spared DRGs. The separate neurons of normal (control) DRG, spared DRG and anti-NT-3 antibody blocking DRG were cultured in vitro respectively. The number of survival neurons and the length of neurites were measured and used for comparison in the control, spared DRG, and block groups.


RESULTS
There were survival neurons and cell clusters in every group. The number of survival neurons and cell clusters of spared DRG group were much larger than those of the control and block groups. The neurite length of neurons, the neurite number and the length of cell clusters of spared DRG group were much greater than those of control and block groups.


CONCLUSIONS
Endogenous NT-3 from spared DRG may act on ganglionic neurons to maintain survival of neuron and stimulate growth of neurite.
Zhang
Wei
W
Department of Histology & Embryology & Neurobiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Zhou
Xue
X
Wang
Ting-hua
TH
Wang
Te-wei
TW
Liu
Su
S
Chen
Si-xiu
SX
Ou
Ke-qun
KQ
D000818:Animals; D002415:Cats; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000166:cytology* / Q000378:metabolism; D008297:Male; D016501:Neurites; D009474:Neurons / Q000166:cytology; D020933:Neurotrophin 3 / Q000378:metabolism*; D011897:Random Allocation; D019051:Rhizotomy; D013126:Spinal Nerve Roots / Q000166:cytology
D020933:Neurotrophin 3