[Effect of fenvalerate on the ovarian calcium homeostasis and the serum steroid hormone].
OBJECTIVE
To observe the effects of fenvalerate (Fen) on ovarian calcium homeostasis.


METHODS
hGLCs were obtained from pre-ovulatory follicles in an in vitro fertilization program, and were cultured for 72 hours. Changes in cellular [Ca(2+)]i induced by Fen in hGLCs were detected with laser scanning confocal microscopy (LSCM) by using the fluorescent Ca(2+) indicator fluo-3/AM. SD female rats were divided into four groups (control, 1/15LD(50), 1/50 LD(50) and 1/250 LD(50)) in experiment. The activity of ovarian Ca(2+)-ATPase and phosphorylase A (P-a) and the contents of calmodulin (CaM) were assessed after a 30-day Fen exposure. In addition, serum estradiol-17 beta (E(2)) and progesterone (P(0)) concentration were measured by radioimmunoassay, which the sampling rats were ensured at diestrus stage before killed according to vaginal smear.


RESULTS
20.0 and 2.0 micromol/L Fen induced the increased of [Ca(2+)]i in hGLC. This [Ca(2+)]i increase mostly resulted from Ca(2+) influx in the studied concentration. Fen had shown the inhibition effects on activity of Ca(2+)-ATPase in 1/250 LD(50) group (P < 0.001) while the activity of phosphorylase A (P-a) in treated groups had significantly enhanced than those of in control. The contents of CaM in ovaries were found to be increased in treated groups. E(2) in 1/250 LD(50) group were higher while P(0) in 1/15 LD(50) group were significantly lower (P < 0.05).


CONCLUSIONS
Exposure to Fen interferes the serum steroid hormone concentrations partly through calcium signal pathway.
He
Jun
J
Institute of Applied Toxicology, Nanjing Medical University, Nanjing 210029, China.
Chen
Jian-feng
JF
Liu
Ru
R
Wang
Yu-bang
YB
Wang
Xin-ru
XR
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D012739:Gonadal Steroid Hormones / Q000097:blood; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism; D006706:Homeostasis / Q000187:drug effects; D006801:Humans; D007306:Insecticides / Q000633:toxicity*; D009570:Nitriles; D010053:Ovary / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011722:Pyrethrins / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley
D012739:Gonadal Steroid Hormones; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D000251:Adenosine Triphosphatases; D002118:Calcium; C017690:fenvalerate