A toxic reagent-free method for normal-phase matrix solid-phase dispersion extraction and reversed-phase liquid chromatographic determination of aldrin, dieldrin, and DDTs in animal fats.
A method for the determination of aldrin, dieldrin, DDT, DDE, and DDD contamination in animal fats (beef tallow, lard, and chicken fat) without using toxic reagents is developed, that uses high-performance liquid chromatography after the sample has been prepared by matrix solid-phase dispersion (MSPD) with acidic alumina oxide. A reversed-phase C(1)-silica column with a mobile phase of 50% (v/v) ethanol solution (in water) and a photo-diode array detector were used for the determination. Average recoveries of the target compounds (0.2-5.0 microg g(-1)) ranged from 84-98%, with coefficients of variation of <5%. The limits of quantitation were </=0.16 microg g(-1 )for AD, </=0.10 microg g(-1) for DD, </=0.06 microg g(-1) for DDT, </=0.07 microg g(-1) for DDE, and </=0.05 microg g(-1 )for DDD. No toxic reagents were used at all.
Graduate School of Human Life Sciences, Osaka City University, Osaka, Japan. furusawa@life.osaka-cu.ac.jp
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Furusawa
D000452:Aldrin / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003634:DDT / Q000032:analysis*; D004026:Dieldrin / Q000032:analysis*; D005223:Fats / Q000737:chemistry*; D008460:Meat
D005223:Fats; D003634:DDT; D004026:Dieldrin; D000452:Aldrin