Apoptosis and nitric oxide release induced by thalidomide, gossypol and dexamethasone in cultured human chronic myelogenous leukemic K-562 cells.
The effects of different dosages of thalidomide, gossypol and dexamethasone on the levels of apoptosis and nitric oxide (NO) production were studied in a human chronic myelogenous leukemic cell line, K-562. Increases in the levels of apoptosis were induced by both 15 and 30 microM of thalidomide but only 15 microM significantly increased NO production. All dosages of gossypol used increased the production of NO and all dosages except 200 microM raised the level of apoptosis. After dexamethasone treatment the level of NO either decreased or stayed constant. Thus, some dosages of thalidomide and gossypol concomitantly raise the levels of apoptosis and NO production, but dexamethasone, though it induced apoptosis, had no significant effect on NO production.
Ergun
Mehmet Ali
MA
Department of Medical Biology and Genetics, Faculty of Medicine, Gazi University, Besevler, Ankara 06510, Turkey.
Konac
Ece
E
Erbas
Deniz
D
Ekmekci
Abdullah
A
D017209:Apoptosis / Q000187:drug effects*; D003272:Contraceptive Agents, Male / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005938:Glucocorticoids / Q000494:pharmacology*; D006072:Gossypol / Q000494:pharmacology*; D006801:Humans; D020014:K562 Cells; D007917:Leprostatic Agents / Q000494:pharmacology*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy* / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D013792:Thalidomide / Q000494:pharmacology*
D003272:Contraceptive Agents, Male; D005938:Glucocorticoids; D007917:Leprostatic Agents; D009569:Nitric Oxide; D013792:Thalidomide; D003907:Dexamethasone; D006072:Gossypol