Molecular urinary sediment analysis in patients with transitional cell bladder carcinoma.
BACKGROUND
Nowadays urinary cytology methods in early diagnosis of superficial bladder transitional carcinoma (TCC) allow the identification of about 35-50% of tumors. Cytoscopy and biopsy are reliable but invasive. It has been recently shown that allelic abnormalities detected by microsatellite analysis of DNA extracted from urine sediment can be successfully used in TCC. We performed a comparative study between urinary cytology and microsatellite sequence alterations in patients affected by TCC.


METHODS
Fifty-eight patients were recruited for the study. Of these, 45 had cystoscopic diagnosis of TCC, while 7 were without apparent lesions after TUR but presented urinary irritative symptoms after BCG endocavitary administration, and 6 who underwent open surgery for benign prostatic hypertrophy represented the control groups. DNA extraction and PCR analysis were performed by using 13 different primers, while urinary cytology was performed by using an Autocyte Preparation System.


RESULTS
Urinary cytology confirmed the presence of TCC in 22% of patients while in 15.5% of them a displastic/inflammatory status was found. Microsatellite analysis allowed the identification of 82% of tumors with a 100% specificity. A high sensitivity was obtained in patients affected by superficial (79%) or G1(80%) tumors.


CONCLUSIONS
Microsatellite analysis represents a highly sensitive and specific marker in TCC diagnosis and monitoring.
Department of Critical Area, Urology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy.
Maurizio
M
Dal Canto
Riccardo
R
Bartoletti
Fabrizio
F
Travaglini
Mauro
M
Piazzini
Giulia
G
Lodovichi
Michelangelo
M
Rizzo
Cesare
C
Selli
D002295:Carcinoma, Transitional Cell / Q000235:genetics / Q000473:pathology / Q000652:urine*; D004273:DNA, Neoplasm / Q000235:genetics / Q000652:urine; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats / Q000235:genetics; D009367:Neoplasm Staging; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000473:pathology / Q000652:urine*; D014556:Urine / Q000737:chemistry / Q000166:cytology
D004273:DNA, Neoplasm