The moss bioreactor.
The production of recombinant proteins in moss bioreactors provides all of the benefits of molecular farming in plants but avoids many plant-specific disadvantages, such as the genetic instability of de-differentiated cells in suspension culture or the lack of containment during field production. Protein yields are in the same range as those of other cell-culture-based production systems. On top of this, the moss Physcomitrella patens is the only known plant that can be genetically modified by homologous recombination, allowing efficient targeted gene disruption. Thus, the major drawback of producing human proteins in plants, allergic reactions caused by plant-specific glycosylation, can be diminished by targeted knockout of the responsible genes in moss. Unlike all other plants, moss allows straightforward 'humanisation' of plant-derived pharmaceuticals.
Decker
Eva L
EL
Plant Biotechnology, University of Freiburg, Schaenzlstrasse 1, 79104 Freiburg, Germany.
Reski
Ralf
R
D019149:Bioreactors; D044002:Bryophyta / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D006031:Glycosylation; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis*
D011994:Recombinant Proteins