Chronic hepatotoxicity of carbon tetrachloride in hsp-70 knock out mice.
The chronic hepatotoxic effects of carbon tetrachloride (CCl(4)) in heat-shock protein (HSP) 70 knock out (HSP70-/-) mice were examined. After repeated intraperitoneal injections of CCl(4) for six weeks, the level of ALT and weight ratio of the liver to body were lower in HSP70-/- mice than in the control (WT) mice. The levels of HSP25 and HSP47 were lowered in HSP70-/- mice as compared with WT mice. The grades of hepatic necrosis and neutrophil infiltration were not significantly different between HSP70-/- and WT mice. The collagen content was not affected significantly by CCl(4) treatment.
Laboratory of Immunopharmacology, Department of Veterinary Medicine, Kangwon National University, Korea.
Dae-Hun
DH
Park
Mi-Sook
MS
Lee
Heui-Jin
HJ
Kim
Han-Seong
HS
Kim
Yun-Lyul
YL
Lee
Myung-Sang
MS
Kwon
Ja-June
JJ
Jang
Min-Jae
MJ
Lee
D000410:Alanine Transaminase / Q000097:blood*; D000818:Animals; D015153:Blotting, Western; D001837:Body Weights and Measures; D002251:Carbon Tetrachloride / Q000633:toxicity*; D004591:Electrophoresis, Polyacrylamide Gel; D050958:HSP47 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000378:metabolism; D020556:Neutrophil Infiltration; D009504:Neutrophils / Q000473:pathology
D050958:HSP47 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C486113:Hsbp1 protein, mouse; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; C494661:Serpinh1 protein, mouse; D002251:Carbon Tetrachloride; D000410:Alanine Transaminase