[Urinary excretion of 4-vinyl phenol after experimental and occupational exposure to styrene].
Aim of this study was to assess the importance and the role of a minor metabolic route of styrene metabolism, involving the oxidation of the arene moiety of styrene, by means of the characterization of the conjugated urinary metabolites of 4-vinylphenol (4-VP). 4-vinylphenol-glucuronide (4-VP-G) and -sulfate (4-VP-S) were measured by liquid chromatography tandem mass spectrometry (LC-MS/MS) from 174 workers belonging to three cohorts recruited in European countries, and from 26 volunteers exposed to 50 mg/m3 (11.8 ppm) of styrene for 8 h. The 4-VP conjugates represented about 0.5-1% of the total excretion of styrene metabolites. Both 4-VP-G and 4-VP-S are eliminated with a mono-phasic kinetic, the glucuronide being excreted faster (half-time, 2.2 +/- 0.2 h) than the sulfate (half-time 9.7 +/- 1.7 h). The urinary 4-VP was found to be significantly correlated both with airborne styrene (r = 0.607, p < 0.001) and the sum of MA and PGA (r = 0.903, p < 0.001 in 'end-of-shift' samples). A measurable background excretion of 4-VP was also found in all urine samples from controls not occupationally exposed to styrene. This background appears to be highly correlated to smoking (p < 0.001). Consequently, the use of 4-VP as a biomarker of styrene exposure is recommended for exposures exceeding 1 ppm.
Laboratorio di Tossicologia Industriale, Centro Studi e Ricerche ISPESL presso l'Università di Parma.
P
P
Manini
G
G
De Palma
R
R
Andreoli
M
M
Goldoni
D
D
Poli
G
G
Lasagni
A
A
Mutti
D000328:Adult; D005260:Female; D006801:Humans; D008297:Male; D016273:Occupational Exposure / Q000032:analysis*; D010636:Phenols / Q000652:urine*; D013343:Styrenes / Q000378:metabolism*
D010636:Phenols; D013343:Styrenes; C030626:4-vinylphenol