Oxidative damage to proteins in yeast cells exposed to adaptive levels of H(2)O(2).
When yeast cells are exposed to sublethal concentrations of oxidants, they adapt to tolerate subsequent lethal treatments. Here, we show that this adaptation involves tolerance of oxidative damage, rather than protection of cellular constituents. o- and m-tyrosine levels are used as a sensitive measure of protein oxidative damage and we show that such damage accumulates in yeast cells exposed to H(2)O(2) at low adaptive levels. Glutathione represents one of the main cellular protections against free radical attack and has a role in adaptation to oxidative stress. Yeast mutants defective in glutathione metabolism are shown to accumulate significant levels of o- and m-tyrosine during normal aerobic growth conditions.
Bioanalytical Mass Spectrometry Facility, Wallace Wurth Building University of New South Wales, Sydney, New South Wales, Australia. a.poljak@unsw.edu.au
Anne
A
Poljak
Ian W
IW
Dawes
Benno A
BA
Ingelse
Mark W
MW
Duncan
George A
GA
Smythe
Chris M
CM
Grant
D000975:Antioxidants / Q000378:metabolism; D002138:Calibration; D002455:Cell Division; D004305:Dose-Response Relationship, Drug; D005609:Free Radicals; D008401:Gas Chromatography-Mass Spectrometry; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D013058:Mass Spectrometry; D009154:Mutation; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D010649:Phenylalanine / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013997:Time Factors; D014443:Tyrosine / Q000737:chemistry / Q000378:metabolism
D000975:Antioxidants; D005609:Free Radicals; D014443:Tyrosine; D010649:Phenylalanine; D006861:Hydrogen Peroxide; D005978:Glutathione; D010100:Oxygen