Hyaluronan-based antiadhesive membrane has no major effect on intraperitoneal growth of colonic tumour cells.
BACKGROUND
A relationship between post-surgical adhesion formation and peritoneal tumour implantation has been proposed. Hyaluronan (HA)-based agents reduce adhesion formation, but the effect on peritoneal tumour is not established. This study investigated the influence of a HA-containing agent on intraperitoneal tumour in an experimental model.


METHODS
66 Balb/c mice underwent laparotomy and damage was inflicted to the parietal peritoneum. The animals were randomized into five groups. Groups 1 and 2 received HA-carboxymethylcellulose bioresorbable membrane and no treatment, respectively. Mice in groups 3-5 were injected intraperitoneally with 10(5) colon 26-B cells after the laparotomy. Treatment consisted of HA membrane, no HA agent and placement of HA membrane on the non-traumatized peritoneal wall, respectively. Animals were killed after 14 days; adhesions were scored in groups 1 and 2, and the tumour mass in groups 3-5. 45 Wag/Rij rats underwent the same procedures and treatment as mice in groups 3-5. In rats, 10(6) CC-531 cells were injected. Rats were killed after 3 weeks and the tumour mass was scored.


RESULTS
HA membrane resulted in a significant reduction of adhesions, but had no major effect on the intraperitoneal tumour mass in mice and rats.


CONCLUSIONS
HA-carboxymethylcellulose bioresorbable membrane has no major effect on intraperitoneal tumour implantation and growth in an experimental model.
Department of Surgery, University Medical Centre St Radboud, Nijmegen, The Netherlands.
C J J M
CJ
Sikkink
V A
VA
Postma
M M P J
MM
Reijnen
B
B
De Man
R P
RP
Bleichrodt
H
H
Van Goor
D000818:Animals; D002266:Carboxymethylcellulose Sodium / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000503:physiopathology*; D006820:Hyaluronic Acid / Q000494:pharmacology*; D007813:Laparotomy / Q000009:adverse effects; D008567:Membranes, Artificial; D051379:Mice; D023421:Models, Animal; D010529:Peritoneal Cavity; D010537:Peritoneum / Q000293:injuries / Q000503:physiopathology; D000267:Tissue Adhesions / Q000209:etiology / Q000517:prevention & control*
D008567:Membranes, Artificial; D006820:Hyaluronic Acid; D002266:Carboxymethylcellulose Sodium