Human reticulon 1-A and 1-B interact with a medium chain of the AP-2 adaptor complex.
Human reticulon family gene 1 (RTN1) is expressed predominantly in neuroendocrine tissues, and produces three proteins termed RTN1-A, RTN1-B, and RTN1-C. Yeast two-hybrid screening indicated that RTN1-A and RTN1-B interacted with AP50, a component of the AP-2 adaptor complex involved in endocytosis. In contrast, RTN1-C did not interact with AP50. Three RTN1 proteins contain the same C-terminal domain. In addition, RTN1-A and RTN1-B share N-terminal 168 amino acid region, suggesting that the 168 amino acid region might play a role in regulating the endocytic process. Although no apparent morphological change of the endocytic organelles was observed, the association of AP50 with the internalized clathrin-coated vesicles was moderately affected when CV-1 cells were directed to express stably RTN1-A or RTN1-B.
Department of Virology, Kurume University School of Medicine, 67 Asahimachi, Kurume, Fukuoka 830-0011, Japan. iwahashi@med.kurume-u.ac.jp
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Iwahashi
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Hamada
D033962:Adaptor Protein Complex 2 / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D004705:Endocytosis; D015870:Gene Expression; D000882:Haplorhini; D006801:Humans; D008954:Models, Biological; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D020798:Two-Hybrid System Techniques
D033962:Adaptor Protein Complex 2; D009419:Nerve Tissue Proteins; C081781:RTN1 protein, human