Effect of Eu(3+) on characterization of photosystem II particles from spinach by spectroscopy.
Photosystem II (PSII) particles were purified from Eu(3+)-treated spinach and studied by spectroscopy. The results showed that electron transport rate of PS II was accelerated by Eu3+ treating, that violet shift of the PSII Soret band or Q-band was 6 nm or 2 nm for the ultraviolet-visible (UV-Vis) spectrum, that the violet shift of the PSII fluorescence emission peak was 9 nm for fluorescence emission spectrum, that the PSII Signal II's of low-temperature electron paramagnetic resonance (EPR) spectrum was intensified under light, and that the PSII CD spectrum was similar to that of control. It is suggested that Eu(3+) might bind to the PSII reaction center complex and enhance the electron transport rate of PSII CD; however, Eu(3+) treatment does not change the configuration of the PSII reaction center complex.
Fashui
Hong
H
College of Life Sciences, Suzhou University, Suzhou, 215006, People's Republic of China.
D002942:Circular Dichroism; D004579:Electron Transport / Q000528:radiation effects; D005063:Europium / Q000494:pharmacology*; D005453:Fluorescence; D008027:Light; D010788:Photosynthesis / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D013057:Spectrum Analysis; D018724:Spinacia oleracea / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects
D045332:Photosystem II Protein Complex; D005063:Europium