title: Distinct mechanisms are involved in tumoristatic and tumoricidal activities of monocyte-derived dendritic cells.
abstract: Monocyte-derived dendritic cells (DC) were found to inhibit proliferation of different tumor cell lines. LPS-induced maturation of DC strongly increased their capacity to inhibit tumor cell growth. We observed that tumoristatic activity of LPS-activated DC was independent of their cytotoxic potential. Indeed, LPS-activated DC were able to inhibit growth of caspase-8-deficient or Bcl-2-overexpressing Jurkat cells whereas they were not cytotoxic towards the same targets. On the other hand, we found that supernatant derived from LPS-activated DC exerted a significant anti-proliferative activity against Jurkat cells while it did not induce any cytotoxic effect. Tumor necrosis factor (TNF) was shown to critically contribute to tumor growth inhibition in this system.
authors.affiliation: Laboratory of Experimental Immunology, Université Libre de Bruxelles ULB, Immunology, 808 route de Lennik, B-1070, Belgium.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Vanderheyde
authors.forename: Peter
authors.initials: P
authors.lastname: Vandenabeele
authors.forename: Michel
authors.initials: M
authors.lastname: Goldman
authors.forename: Fabienne
authors.initials: F
authors.lastname: Willems
mesh_terms: D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects; D009369:Neoplasms / Q000276:immunology* / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha