title: Cryopreservation of isolated ovine primordial follicles with propylene glycol and glycerol.
abstract: OBJECTIVE
  To verify the viability of isolated primordial follicles to different propylene glycol (PROH) and glycerol (GLY) concentrations before and after cryopreservation.
  
  
  METHODS
  Isolated primordial follicles were stained with trypan blue to evaluate the effect of different PROH and GLY concentrations before and after cryopreservation.
  
  
  METHODS
  Laboratorio Renzo Giuliani, University of Florence, Italy.
  
  
  METHODS
  Thirty- to forty-day-old lambs.
  
  
  METHODS
  : Isolation of primordial follicles with subsequent exposure to cryoprotectant and freezing.
  
  
  METHODS
  Histologic structure and follicular mortality.
  
  
  RESULTS
  After the isolation procedure (control), the mean number of live primordial follicles/mL was 2,688 and 4,452 in the GLY and PROH groups, respectively. When GLY was used, the number of live follicles before cryopreservation was 820, 756, 640, 524, 564, and 460 follicles/mL with concentrations of 0, 0.5, 1.0, 1.5, 2.0, and 2.5 mol/L, respectively. After cryopreservation, this number decreased to 0, 12, 36, 100, 84, and 68 follicles/mL, respectively, with the same concentrations. When PROH was used, the number of live follicles before cryopreservation was 4,216, 3,880, 3,560, 1,812, 704, and 568 follicles/mL with concentrations of 0, 0.5, 1.0, 1.5, 2.0, and 2.5 mol/L, respectively. After cryopreservation, this number decreased to 0, 116, 336, 472, 360, and 244 follicles/mL, respectively, with the same concentrations.
  
  
  CONCLUSIONS
  Both cryoprotectants were shown to preserve isolated primordial follicles after cryopreservation.
authors.affiliation: University of Santa Maria, Santa Maria, RS, Brazil. amorimc_kleinj@yahoo.com
authors.forename: Christiani Andrade
authors.initials: CA
authors.lastname: Amorim
authors.forename: Davide
authors.initials: D
authors.lastname: Rondina
authors.forename: Ana Paula Ribeiro
authors.initials: AP
authors.lastname: Rodrigues
authors.forename: Paulo Bayard Dias
authors.initials: PB
authors.lastname: Gonçalves
authors.forename: José Ricardo
authors.initials: JR
authors.lastname: de Figueiredo
authors.forename: Alessandro
authors.initials: A
authors.lastname: Giorgetti
mesh_terms: D000818:Animals; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005990:Glycerol / Q000008:administration & dosage*; D006080:Ovarian Follicle / Q000033:anatomy & histology / Q000187:drug effects* / Q000502:physiology; D019946:Propylene Glycol / Q000008:administration & dosage*; D012756:Sheep; D014022:Tissue Survival / Q000187:drug effects; D018675:Toxicity Tests
chemical_list: D003451:Cryoprotective Agents; D019946:Propylene Glycol; D005990:Glycerol