Target-assisted iterative screening of phage surface display cDNA libraries.
The novel screening format, target-assisted iterative screening (TAIS), comprises a simple and rapid two-step procedure for in vitro affinity selection of specific binders from enormous molecular diversities to the target molecule of interest. This detailed protocol describes the application of TAIS to a T7 phage-displayed complementary DNA (cDNA) library with a protein domain as a target. Protocols for purification of the target as glutathione-S-transferase (GST) fusion protein and modifications of the purified target that are required for the screening complement the TAIS protocol. The described application is a method of choice for the researchers interested in the identification and characterization of novel protein-protein interactions mediated by peptide recognition domains.
Kurakin
Alexei
A
Buck Institute for Age Research, Novato, CA, USA.
Wu
Susan
S
Bredesen
Dale E
DE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015723:Gene Library; D006801:Humans; D019151:Peptide Library; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D019151:Peptide Library; D011993:Recombinant Fusion Proteins