title: Effect of weightbearing on bone formation during distraction osteogenesis.
abstract: The effect of weightbearing on the bone formation during distraction osteogenesis was studied with 24 goats after standardized osteotomy and distraction. Twelve goats were allowed to bear weight immediately and 12 were not allowed to bear weight. With the pattern of weightbearing documented, the formation of bone was monitored with serial radiographs and the bone forming activity was studied with histologic examination and immunohistochemical study of transforming growth factor-beta 1 expression. The results of the study showed that the bone formation as depicted by radiologic assessment of callus width and mineralized tissue was significantly better in the weightbearing group. Histologic study showed more bone formation qualitatively and quantitatively in the weightbearing group. This study confirmed the beneficial effect of weightbearing activity during distraction osteogenesis. Distraction provides pulsed form stimulation by tension stress across the osteotomy site and initiates osteogenesis while the compression stress exerted by the weightbearing is continuous in daily activities and enhances new bone formation. The clinical practice of early weightbearing during distraction osteogenesis should be reinforced.
authors.affiliation: Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Shatin, Hong Kong, China. ksleung@cuhk.edu.hk
authors.forename: Kwok-Sui
authors.initials: KS
authors.lastname: Leung
authors.forename: Wing-Hoi
authors.initials: WH
authors.lastname: Cheung
authors.forename: Hiu-Yan
authors.initials: HY
authors.lastname: Yeung
authors.forename: Kwong-Man
authors.initials: KM
authors.lastname: Lee
authors.forename: Kwok-Pui
authors.initials: KP
authors.lastname: Fung
mesh_terms: D000818:Animals; D001707:Biopsy, Needle; D002146:Bony Callus / Q000000981:diagnostic imaging* / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D006041:Goats; D007150:Immunohistochemistry; D008297:Male; D010012:Osteogenesis / Q000502:physiology*; D019857:Osteogenesis, Distraction / Q000379:methods*; D011336:Probability; D011859:Radiography; D012680:Sensitivity and Specificity; D016212:Transforming Growth Factor beta / Q000032:analysis; D053773:Transforming Growth Factor beta1; D016474:Weight-Bearing
chemical_list: D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1