title: Human immunoglobulin G mediates protective immunity and identifies protective antigens against larval Strongyloides stercoralis in mice.
abstract: Protective immunity to larval Strongyloides stercoralis in mice has been shown to be dependent on antibody, complement, and granulocytes. The goals of the present study was to determine the following: (1) whether human serum could passively transfer immunity to mice, (2) the mechanism by which the serum mediated killing, and (3) whether the antigens (Ags) recognized by the protective human antibody could induce protective immunity in mice. Immunoglobulin G (IgG) from a S. stercoralis-seropositive individual passively transferred immunity to mice. The antibody required granulocytes, but not eosinophils, and complement activation to kill the larvae. Antibody-dependent cellular cytotoxicity was not required for larval killing. Immunization of mice with soluble larval Ags isolated by use of the protective immune IgG resulted in protective immunity. In conclusion, immunity could be transferred to mice by IgG from immune humans, and Ags identified by the immune human IgG induced protective immunity in mice, which thereby suggests their possible use in a vaccine against this infection.
authors.affiliation: Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
authors.forename: Laura A
authors.initials: LA
authors.lastname: Kerepesi
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Nolan
authors.forename: Gerhard A
authors.initials: GA
authors.lastname: Schad
authors.forename: Sara
authors.initials: S
authors.lastname: Lustigman
authors.forename: De'Broski R
authors.initials: DR
authors.lastname: Herbert
authors.forename: Paul B
authors.initials: PB
authors.lastname: Keiser
authors.forename: Thomas B
authors.initials: TB
authors.lastname: Nutman
authors.forename: Alejandro J
authors.initials: AJ
authors.lastname: Krolewiecki
authors.forename: David
authors.initials: D
authors.lastname: Abraham
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D002846:Chromatography, Affinity; D003176:Complement C3 / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007116:Immunization, Passive / Q000379:methods*; D007074:Immunoglobulin G / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016253:Microscopy, Immunoelectron; D017452:Receptors, IgG / Q000276:immunology; D017171:Strongyloides stercoralis / Q000276:immunology* / Q000648:ultrastructure; D013322:Strongyloidiasis / Q000276:immunology* / Q000469:parasitology
chemical_list: D000947:Antigens, Helminth; D003176:Complement C3; D007074:Immunoglobulin G; D017452:Receptors, IgG