title: Apoptotic and anti-adhesion effect of ajoene, a garlic derived compound, on the murine melanoma B16F10 cells: possible role of caspase-3 and the alpha(4)beta(1) integrin.
abstract: In this study we evaluated the hypothesis that the antitumor activity of ajoene could be associated with its apoptosis-inducing effect, and with its ability to block the expression of the alpha(4)beta(1) integrin, in the murine melanoma B16F10 cells. Ajoene induced a significant reduction in B16F10 viability (IC(50)=62 microM), in a dose-dependent manner. Flow cytometric analysis showed that the cytotoxic effect of this compound was associated with caspase-3 activation. Ajoene at 25 microM altered the alpha(4)beta(1) integrin expression on B16F10, and induced a significant reduction in the adhesion of these cells to an endothelial cell monolayer.
authors.affiliation: Laboratorio de Trombosis Experimental, Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas, Caracas 1020-A, Venezuela.
authors.forename: Eliades
authors.initials: E
authors.lastname: Ledezma
authors.forename: Rafael
authors.initials: R
authors.lastname: Apitz-Castro
authors.forename: José
authors.initials: J
authors.lastname: Cardier
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects*; D003114:Colony-Forming Units Assay; D004220:Disulfides / Q000494:pharmacology*; D005434:Flow Cytometry; D005737:Garlic / Q000737:chemistry; D005980:Glutathione Reductase / Q000037:antagonists & inhibitors; D039041:Integrin alpha4beta1 / Q000378:metabolism*; D008546:Melanoma, Experimental / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D013454:Sulfoxides; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D004220:Disulfides; D039041:Integrin alpha4beta1; D010936:Plant Extracts; D013454:Sulfoxides; C048980:ajoene; D005980:Glutathione Reductase; C505409:Casp3 protein, mouse; D053148:Caspase 3; D020169:Caspases