title: CD38 low IgG-secreting cells are precursors of various CD38 high-expressing plasma cell populations.
abstract: Despite the important role immunoglobulin G (IgG)-secreting plasma cells play in memory immune responses, the differentiation and homeostasis of these cells are not completely understood. Here, we studied the differentiation of human IgG-secreting cells ex vivo and in vitro, identifying these cells by the cellular affinity matrix technology. Several subpopulations of IgG-secreting cells were identified among the cells isolated from tonsils and bone marrow, particularly differing in the expression levels of CD9, CD19, and CD38. CD38 low IgG-secreting cells were present exclusively in the tonsils. A major fraction of these cells appeared to be early plasma cell precursors, as upon activation of B cells in vitro, IgG secretion preceded up-regulation of CD38, and on tonsillar sections, IgG-containing, CD38 low cells with a plasmacytoid phenotype were found in follicles, where plasma cell differentiation starts. A unitary phenotype of migratory peripheral blood IgG-secreting cells suggests that all bone marrow plasma cell populations share a common precursor cell. These data are compatible with a multistep model for plasma cell differentiation and imply that a common CD38 low IgG-secreting precursor gives rise to a diverse plasma cell compartment.
authors.affiliation: Deutsches Rheuma-Forschungszentrum Berlin, Schumannstrasse 20/21, D-10117 Berlin, Germany.
authors.forename: Sergio
authors.initials: S
authors.lastname: Arce
authors.forename: Elke
authors.initials: E
authors.lastname: Luger
authors.forename: Gwendolin
authors.initials: G
authors.lastname: Muehlinghaus
authors.forename: Giuliana
authors.initials: G
authors.lastname: Cassese
authors.forename: Anja
authors.initials: A
authors.lastname: Hauser
authors.forename: Alexander
authors.initials: A
authors.lastname: Horst
authors.forename: Katja
authors.initials: K
authors.lastname: Lehnert
authors.forename: Marcus
authors.initials: M
authors.lastname: Odendahl
authors.forename: Dirk
authors.initials: D
authors.lastname: Hönemann
authors.forename: Karl-Dieter
authors.initials: KD
authors.lastname: Heller
authors.forename: Harald
authors.initials: H
authors.lastname: Kleinschmidt
authors.forename: Claudia
authors.initials: C
authors.lastname: Berek
authors.forename: Thomas
authors.initials: T
authors.lastname: Dörner
authors.forename: Veit
authors.initials: V
authors.lastname: Krenn
authors.forename: Falk
authors.initials: F
authors.lastname: Hiepe
authors.forename: Ralf
authors.initials: R
authors.lastname: Bargou
authors.forename: Andreas
authors.initials: A
authors.lastname: Radbruch
authors.forename: Rudolf A
authors.initials: RA
authors.lastname: Manz
mesh_terms: D036541:ADP-ribosyl Cyclase / Q000378:metabolism*; D051997:ADP-ribosyl Cyclase 1; D015703:Antigens, CD / Q000378:metabolism*; D018941:Antigens, CD19 / Q000378:metabolism; D016175:B-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D001853:Bone Marrow / Q000276:immunology*; D002454:Cell Differentiation; D002465:Cell Movement; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D066298:In Vitro Techniques; D008562:Membrane Glycoproteins / Q000378:metabolism; D014066:Palatine Tonsil / Q000166:cytology / Q000276:immunology*; D010641:Phenotype; D010950:Plasma Cells / Q000276:immunology* / Q000378:metabolism; D060245:Tetraspanin 29
chemical_list: D015703:Antigens, CD; D018941:Antigens, CD19; C554869:CD9 protein, human; D007074:Immunoglobulin G; D008562:Membrane Glycoproteins; D060245:Tetraspanin 29; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1