title: Phenotypic characterization of a human synovial sarcoma cell line, SW982, and its response to dexamethasone.
abstract: SW982 cells are characterized by expression of inflammatory cytokine and matrix metalloproteinase (MMP) genes and by their response to dexamethasone at different cell densities. They express genes encoding interleukin (IL)-1 beta; IL-6; transforming growth factor-beta; intercellular adhesion molecule-1; cycloxygenase (COX)-2; and MMPs, including MMP-1, MMP-2, MMP-13, and MT1-MMP; tissue inhibitor of metalloproteinase-2; and a disintegrin and metalloproteinase with thrombospondin motifs-4. Expression of all the genes examined was induced with 2 ng/ml IL-1 beta at low cell density. The cells, however, failed to express tumor necrosis factor-alpha, COX-1, and MMP-9, regardless of the presence of IL-1 beta. Dexamethasone significantly reduced IL-1 beta, IL-6, COX-2, and MMP-1 expression at high cell density. The results suggest that SW982 cells are a useful tool for studying the expression of inflammatory cytokine or MMP genes.
authors.affiliation: Department of Cellular and Molecular Physiology, Kohno Clinical Medicine Research Institute, 3-4-4, Kita-Shinagawa, Tokyo 140-0001, Japan.
authors.forename: Taisuke
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokoyama
authors.forename: Hiromi
authors.initials: H
authors.lastname: Akatsu
authors.forename: Takashi
authors.initials: T
authors.lastname: Tukiyama
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Tokiwa
mesh_terms: D045744:Cell Line, Tumor / Q000187:drug effects* / Q000276:immunology; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D020782:Matrix Metalloproteinases / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D013584:Sarcoma, Synovial / Q000235:genetics / Q000276:immunology / Q000473:pathology*
chemical_list: D016207:Cytokines; D005938:Glucocorticoids; D003907:Dexamethasone; D020782:Matrix Metalloproteinases