title: Loss of translational control in yeast compromised for the major mRNA decay pathway.
abstract: The cytoplasmic fate of mRNAs is dictated by the relative activities of the intimately connected mRNA decay and translation initiation pathways. In this study, we have found that yeast strains compromised for stages downstream of deadenylation in the major mRNA decay pathway are incapable of inhibiting global translation initiation in response to stress. In the past, the paradigm of the eIF2alpha kinase-dependent amino acid starvation pathway in yeast has been used to evaluate this highly conserved stress response in all eukaryotic cells. Using a similar approach we have found that even though the mRNA decay mutants maintain high levels of general translation, they exhibit many of the hallmarks of amino acid starvation, including increased eIF2alpha phosphorylation and activated GCN4 mRNA translation. Therefore, these mutants appear translationally oblivious to decreased ternary complex abundance, and we propose that this is due to higher rates of mRNA recruitment to the 40S ribosomal subunit.
authors.affiliation: Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, Manchester M60 1QD, United Kingdom.
authors.forename: L E A
authors.initials: LE
authors.lastname: Holmes
authors.forename: S G
authors.initials: SG
authors.lastname: Campbell
authors.forename: S K
authors.initials: SK
authors.lastname: De Long
authors.forename: A B
authors.initials: AB
authors.lastname: Sachs
authors.forename: M P
authors.initials: MP
authors.lastname: Ashe
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004722:Endoribonucleases / Q000235:genetics / Q000378:metabolism; D039603:Eukaryotic Initiation Factor-4G / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D009154:Mutation; D014176:Protein Biosynthesis; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D039381:RNA Cap-Binding Proteins; D012331:RNA, Fungal / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000596:Amino Acids; D004268:DNA-Binding Proteins; D039603:Eukaryotic Initiation Factor-4G; C114069:LSM1 protein, S cerevisiae; D039381:RNA Cap-Binding Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; D011494:Protein Kinases; C100868:DCP1 protein, S cerevisiae; D004722:Endoribonucleases; D005947:Glucose