title: [Co-expression of two spliceosomes of cyclin-dependent kinase-2 in SH-SY5Y cells].
abstract: OBJECTIVE
  To investigate the expression of cyclin-dependent kinase-2 (CDK-2) gene in SH-SY5Y cells.
  
  
  METHODS
  The expression of CDK-2 gene was examined with reverse transcriptional (RT)-PCR, and the PCR products underwent electrophoresis on non-denaturing poly-acrylamide gel (PAG) followed by silver staining. The separated and purified DNAs were ligated into pMD18-T vector, and the positive clones identified by sequence analysis.
  
  
  RESULTS
  Two DNA bands were displayed on PAG, and the one with smaller molecular weight was less intensively stained. The sequences of the two clones indicated that both were products of CDK-2 gene.
  
  
  CONCLUSIONS
  Two kinds of CDK-2 gene products are co-expressed in the SH-SY5Y cells, one of which lacks the fifth exon and is expressed at a low level.
authors.affiliation: Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China. yue-feng@fimmu.com
authors.forename: Feng
authors.initials: F
authors.lastname: Yue
authors.forename: Wen-li
authors.initials: WL
authors.lastname: Ma
authors.forename: Bao
authors.initials: B
authors.lastname: Zhang
authors.forename: Yan-bin
authors.initials: YB
authors.lastname: Song
authors.forename: Wen-ling
authors.initials: WL
authors.lastname: Zheng
mesh_terms: D042846:CDC2-CDC28 Kinases / Q000235:genetics*; D045744:Cell Line, Tumor; D051357:Cyclin-Dependent Kinase 2; D005434:Flow Cytometry; D006801:Humans; D009447:Neuroblastoma / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D017381:Spliceosomes
chemical_list: D042846:CDC2-CDC28 Kinases; C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2