title: [Detection of oprI gene of Pseudomonas aeruginosa by reverse dot-blot hybridization].
abstract: OBJECTIVE
  To establish a rapid method for detecting Pseudomonas aeruginosa at the early stage of infection.
  
  
  METHODS
  Specific primers were designed according to oprI gene sequence of Pseudomonas aeruginosa, and the specific probe was synthesized by PCR. After photosensitive biotin labeling of the bacterial DNA, reverse dot-blot hybridization was used to detect Pseudomonas aeruginosa.
  
  
  RESULTS
  The probe synthesized was highly specific to Pseudomonas aeruginosa without cross reaction with other bacteria, viruses or fungi. The method was capable of detecting 100 ng bacteria DNA.
  
  
  CONCLUSIONS
  Reverse dot-blot hybridization possesses the merits of speediness and specificity in the detection of Pseudomonas aeruginosa in the early stage of infection.
authors.affiliation: Department of Respiratory Diseases, Guangzhou General Hospital of Guangzhou Command, Guangzhou 510010, China. huizhen2000@163.com
authors.forename: Hui-zhen
authors.initials: HZ
authors.lastname: Fan
authors.forename: Wen-jie
authors.initials: WJ
authors.lastname: Huang
authors.forename: Kun
authors.initials: K
authors.lastname: Liang
authors.forename: Yi
authors.initials: Y
authors.lastname: Fang
authors.forename: Li-ren
authors.initials: LR
authors.lastname: Ma
authors.forename: Yao-qing
authors.initials: YQ
authors.lastname: Liu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D008074:Lipoproteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D012680:Sensitivity and Specificity
chemical_list: D001426:Bacterial Proteins; D008074:Lipoproteins; C060507:outer membrane lipoprotein I, Bacteria