Use of bifunctional cross-linking reagents in mapping genomic distribution of chromatin remodeling complexes.
Chromatin remodeling complexes consist of multiple subunits, some of which are in intimate contact with DNA while others are not. The ability to effectively cross-link proteins to DNA with formaldehyde is impacted by the average distance between the two species. Productive cross-linking of proteins not in direct contact with DNA is greatly facilitated by the inclusion of an initial cross-linking reaction using bifunctional imidoester cross-linking reagents.
Fujita
Naoyuki
N
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Whitehead Building, Room 142, 615 Michael Street, Atlanta, GA 30322, USA.
Wade
Paul A
PA
D000818:Animals; D002478:Cells, Cultured; D002843:Chromatin / Q000235:genetics* / Q000502:physiology; D042002:Chromatin Assembly and Disassembly / Q000235:genetics* / Q000502:physiology; D002874:Chromosome Mapping / Q000379:methods*; D003432:Cross-Linking Reagents; D023281:Genomics / Q000379:methods*; D006801:Humans; D011401:Promoter Regions, Genetic
D002843:Chromatin; D003432:Cross-Linking Reagents