title: Development of hybrid materials based on hydroxyethylmethacrylate as supports for improving cell adhesion and proliferation.
abstract: A novel hydrogel based on 2-hydroxyethylmethacrylate and fumed silica nanoparticles is presented. The filler was mixed at increasing amount (3-40% w/w) to the organic monomer, before accomplish thermal polymerization. The hybrid composite materials obtained were characterized as far as concern the physical-chemical stability and sorption behaviour in water and water solutions. The novel hybrid hydrogels were compared to poly(hydroxyethylmethacrylate) (pHEMA) on cytocompatibility and ability to elicit cell adhesion and proliferation. These in vitro assays showed that the first ones were supporting cell growth better then pHEMA, moreover experiments on murine fibroblasts showed improved adhesion and proliferation with the increase of the nanomeric filler content. For a more physiological response, the in vitro tests should match biomaterials with cell populations typical of the implant site. Therefore, in view of future applications of these composites as scaffolds for bone engineering, in a successive step of our research we selected primary cultures of human osteoblasts (OB) as the most appropriate models to study the in vitro performance of these materials. The preliminary results obtained confirmed the remarkable improvement of OB adhesion properties of the new hybrids with respect to pure pHEMA.
authors.affiliation: Section of Biotechnology and Molecular Biology, Department of Experimental Medicine, Second University of Naples, via De Crecchio 7, 80138 Naples, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Schiraldi
authors.forename: Antonella
authors.initials: A
authors.lastname: D'Agostino
authors.forename: Adriana
authors.initials: A
authors.lastname: Oliva
authors.forename: Floriana
authors.initials: F
authors.lastname: Flamma
authors.forename: Alfredo
authors.initials: A
authors.lastname: De Rosa
authors.forename: Antonio
authors.initials: A
authors.lastname: Apicella
authors.forename: Raffaella
authors.initials: R
authors.lastname: Aversa
authors.forename: Mario
authors.initials: M
authors.lastname: De Rosa
mesh_terms: D000328:Adult; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D018786:Bone Substitutes / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D008297:Male; D008420:Manufactured Materials / Q000032:analysis; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D020100:Hydrogels; D008689:Methacrylates; D014867:Water; C005044:hydroxyethyl methacrylate