title: Dual inhibition of cyclooxygenase-1 and 5-lipoxygenase by aerial part of Bupleurum fruticescens methanol extract.
abstract: The effect of the methanol extract from aerial parts of Bupleurum fruticescens on the release of eicosanoids and hydrolytic enzymes was determined on in vitro cell systems. The extract had a significant effect on 5-lipoxygenase (5-LOX) activity, inhibiting both LTB4 and 5(S)-HETE production with IC50 values of 112 microg/ml and 95 microg/ml, respectively. At concentrations of 200 microg/ml, the extract also inhibited cyclooxygenase-1 (90%) and elastase activities (54%). The 12-LOX activity in intact platelets was not affected; a fact, which suggests that phospholipase A2 (PLA2) activity, is not modified by the extract.
authors.affiliation: Departament de Farmacologia, Facultat de Farmàcia, Universitat de València, Avenida Vicent Andrés Estellés, s/n. 46100 Burjassot, Valencia, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Prieto
authors.forename: R M
authors.initials: RM
authors.lastname: Giner
authors.forename: M C
authors.initials: MC
authors.lastname: Recio
authors.forename: S
authors.initials: S
authors.lastname: Máñez
authors.forename: J L
authors.initials: JL
authors.lastname: Ríos
mesh_terms: D000818:Animals; D029972:Bupleurum; D051545:Cyclooxygenase 1; D016861:Cyclooxygenase Inhibitors / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D035264:Flowers; D006801:Humans; D020128:Inhibitory Concentration 50; D007527:Isoenzymes / Q000037:antagonists & inhibitors*; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors*; D008084:Lipoxygenase / Q000187:drug effects*; D008565:Membrane Proteins; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots; D011451:Prostaglandin-Endoperoxide Synthases; D051381:Rats; D017208:Rats, Wistar
chemical_list: C051274:Bupleurum root extract; D016861:Cyclooxygenase Inhibitors; D007527:Isoenzymes; D008565:Membrane Proteins; D010936:Plant Extracts; D008084:Lipoxygenase; D051545:Cyclooxygenase 1; C496511:PTGS1 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; C496513:Ptgs1 protein, rat; D019272:Leukocyte Elastase