Localization of vitellogenin mRNA expression and vitellogenin uptake during ovarian maturation in the giant freshwater prawn Macrobrachium rosenbergii.
In situ hybridization and immunohistochemical techniques were used to investigate the dynamics of vitellogenin (Vg) mRNA expression and Vg uptake during ovarian maturation in the hepatopancreas and ovary at differing stages of ovarian maturation in both intact and eyestalk ablated female Macrobrachium rosenbergii. In the hepatopancreas of intact animals, Vg mRNA expression was detected faintly two days after ecdysis, and signals showed a gradual increase as the molt cycle advanced to the premolt stages, but decreased at the late premolt stage. Vg mRNA was detected in the R-cells of the hepatopancreas, indicating that these cells are responsible for synthesizing Vg. No Vg mRNA expression was observed in the ovary. Immunohistochemistry results for the hepatopancreas showed a pattern of staining intensity similar to that of in situ hybridization. Increases in the accumulation of yolk protein in the oocytes occurred concomitantly with increasing Vg mRNA expression. In eyestalk ablated animals, Vg mRNA expression and Vg uptake showed similar but accelerated patterns to those of intact animals. This study has confirmed on the cellular level previous results that Vg synthesis is intrinsically correlated to ovarian maturation and the molt cycle in M. rosenbergii.
Jasmani
Safiah
S
Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki 305-8686, Japan.
Ohira
Tsuyoshi
T
Jayasankar
Vidya
V
Tsutsui
Naoaki
N
Aida
Katsumi
K
Wilder
Marcy N
MN
D000818:Animals; D005260:Female; D015870:Gene Expression; D043143:Hepatopancreas / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D010053:Ovary / Q000254:growth & development* / Q000378:metabolism; D033541:Palaemonidae / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D014819:Vitellogenins / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D014819:Vitellogenins