title: Imaging of bioluminescent LNCaP-luc-M6 tumors: a new animal model for the study of metastatic human prostate cancer.
abstract: BACKGROUND
  Animal experiments examining hormone-sensitive metastatic prostate cancer using the human LNCaP cell line have been limited to endpoint analyses. To permit longitudinal studies, we generated a luciferase-expressing cell line and used bioluminescent imaging (BLI) to non-invasively monitor the in vivo growth of primary LNCaP tumors and metastasis.
  
  
  METHODS
  LNCaP.FGC cells were transfected to constitutively express firefly luciferase. LNCaP-luc-M6 cells were tested for bioluminescent signal intensity and hormone responsiveness in vitro. The cells were implanted in subcutaneous and orthotopic sites in SCID-bg mice and imaged over time.
  
  
  RESULTS
  The LNCaP-luc-M6 cells formed subcutaneous and orthotopic tumors in SCID-bg mice, and nearly all tumor-bearing animals developed pulmonary metastases. Early detection and temporal growth of primary tumors and metastatic lesions was successfully monitored by BLI.
  
  
  CONCLUSIONS
  The LNCaP-luc-M6 cell line is a bioluminescent, hormone-sensitive prostate cancer cell line applicable for BLI studies to non-invasively monitor subcutaneous and orthotopic prostate tumor growth and metastasis in vivo.
authors.affiliation: Xenogen Corporation, Alameda, California 94501, USA.
authors.forename: Caroline D
authors.initials: CD
authors.lastname: Scatena
authors.forename: Mischa A
authors.initials: MA
authors.lastname: Hepner
authors.forename: Yoko A
authors.initials: YA
authors.lastname: Oei
authors.forename: Joan M
authors.initials: JM
authors.lastname: Dusich
authors.forename: Shang-Fan
authors.initials: SF
authors.lastname: Yu
authors.forename: Tony
authors.initials: T
authors.lastname: Purchio
authors.forename: Pamela R
authors.initials: PR
authors.lastname: Contag
authors.forename: Darlene E
authors.initials: DE
authors.lastname: Jenkins
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis* / Q000235:genetics*; D008163:Luminescent Measurements; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009362:Neoplasm Metastasis / Q000503:physiopathology*; D010641:Phenotype; D010957:Plasmids; D011471:Prostatic Neoplasms / Q000473:pathology* / Q000662:veterinary; D014162:Transfection; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
chemical_list: D008156:Luciferases