Toxicogenetic evaluation of metronidazole in the treatment of women infected with Trichomonas vaginalis.
Samples of peripheral blood were collected once from non-smoking women who were healthy (controls) and twice (immediately before and immediately after 7 days of treatment with metronidazole at 500 mg/day) from non-smoking women infected with Trichomonas vaginalis. Lymphocyte cultures were prepared and used, in toxicogenetic studies, to determine the frequency of sister-chromatid exchange (SCE), the mitotic index (MI), and the replication index (RI) for each sample. MTZ treatment of the infected women led to an increase in the frequency of SCE (P <0.001), a decrease in the MI (P <0.003), and a modification in the kinetics of cell proliferation, with a decrease in the RI (P <0.0006). The differences seen between the results for the controls and those for the infected women, before and after MTZ treatment, may be attributed to the presence of the parasite, to the treatment itself, and/or to variation in the host's response to infection with T. vaginalis.
Carballo
M A
MA
Citogenética y Genética Toxicológica (CIGETOX), Departamento de Bioqúimica Clínica, Facultad de Farmacia y Bioqúimica (FFyB), Universidad de Buenos Aires, Junín 956, (1113), Buenos Aires, Argentina. macarballo@dbc.ffyb.uba.ar
Palermo
A M
AM
Mudry
M D
MD
D000328:Adult; D000994:Antitrichomonal Agents / Q000009:adverse effects*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D008795:Metronidazole / Q000009:adverse effects*; D008940:Mitotic Index / Q000379:methods; D012854:Sister Chromatid Exchange / Q000187:drug effects / Q000235:genetics; D014247:Trichomonas Vaginitis / Q000188:drug therapy* / Q000235:genetics
D000994:Antitrichomonal Agents; D008795:Metronidazole