Motion of spin label side chains in cellular retinol-binding protein: correlation with structure and nearest-neighbor interactions in an antiparallel beta-sheet.
A goal in the development of site-directed spin labeling in proteins is to correlate the motion of a nitroxide side chain with local structure, interactions, and dynamics. Significant progress toward this goal has been made using alpha-helical proteins of known structure, and the present study is the first step in a similar exploration of a beta-sheet protein, cellular retinol-binding protein (CRBP). Nitroxide side chains were introduced along both interior and edge strands. At sites in interior strands, the side-chain motion is strongly influenced by interactions with side chains of neighboring strands, giving rise to a rich variety of dynamic modes (weakly ordered, strongly ordered, immobilized) and complex electron paramagnetic resonance spectra that are modulated by strand twist. The interactions giving rise to the dynamic modes are explored using mutagenesis, and the results demonstrate the particular importance of the non-hydrogen-bonded neighbor residue in giving rise to highly ordered states. Along edge strands of the beta-sheet, the motion of the side chain is simple and weakly ordered, resembling that at solvent-exposed surfaces of an alpha-helix. A simple working model is proposed that can account for the wide variety of dynamic modes encountered. Collectively, the results suggest that the nitroxide side chain is an effective probe of side-chain interactions, and that site-directed spin labeling should be a powerful means of monitoring conformational changes that involve changes in beta-sheet topology.
Lietzow
Michael A
MA
Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-7008, USA.
Hubbell
Wayne L
WL
D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D006860:Hydrogen Bonding; D007532:Isoleucine / Q000235:genetics; D008956:Models, Chemical; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009589:Nitrogen Oxides / Q000737:chemistry; D017433:Protein Structure, Secondary / Q000235:genetics; D051381:Rats; D012177:Retinol-Binding Proteins / Q000737:chemistry* / Q000235:genetics; D054840:Retinol-Binding Proteins, Cellular; D012997:Solvents; D013113:Spin Labels; D055672:Static Electricity; D013329:Structure-Activity Relationship; D013816:Thermodynamics
D009589:Nitrogen Oxides; D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular; D012997:Solvents; D013113:Spin Labels; D007532:Isoleucine; C039900:nitroxyl