Serum protein-expression profiling using the ProteinChip biomarker system.
Protein-expression profiling of serum is a common approach to the discovery of potential diagnostic and therapeutic markers of disease. Like any other proteome, the serum proteome is characterized by protein expression across a large dynamic range. This single facet requires the employment of fractionation procedures prior to detection of protein. The authors use a combination of conventional column chromatography with array-based chromatography to simplify the serum proteome into subproteomes, thus providing a greater representation of the serum proteome. Robotics is employed to increase the throughput of sample processing. These procedures result in large amounts of data that are analyzed through a series of preprocessing and postprocessing steps. A well-designed serum profiling project can therefore result in the discovery of statistically sound, clinically meaningful protein biomarkers.
Gilbert
Kate
K
Ciphergen Biosystems, Inc, Fremont, CA, USA.
Figueredo
Sharel
S
Meng
Xiao-Ying
XY
Yip
Christine
C
Fung
Eric T
ET
D000837:Anion Exchange Resins; D001798:Blood Proteins / Q000737:chemistry* / Q000378:metabolism; D002852:Chromatography, Ion Exchange / Q000379:methods; D020869:Gene Expression Profiling; D006801:Humans; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D020543:Proteome / Q000032:analysis*
D000837:Anion Exchange Resins; D001798:Blood Proteins; D020543:Proteome