Human PRMT5 expression is enhanced during in vitro tubule formation and after in vivo ischemic injury in renal epithelial cells.
BACKGROUND
The interactions between cells and the extracellular matrix (ECM) are important in the regulation of cell growth and differentiation. Cells cultured in ECM differentiate and develop tubular structures. The kidney has the ability to partially recover function after an ischemic insult through repairing its tubular epithelium. The factors that contribute to tubule formation in vitro may mediate tubule regeneration in the recovery stage of acute tubular necrosis.


METHODS
RNA purified from cells grown on plastic, on Matrigel and in Matrigel matrix were subjected to differential display analysis to identify the transcripts that are differentially expressed during in vitro tubulogenesis.


RESULTS
Protein arginine methyltransferase 5 (PRMT5) expression increased in renal epithelial cells undergoing tubule formation. PRMT5 expression is developmentally regulated and ubiquitously expressed in a variety of adult tissues. We also demonstrated that expression of PRMT5 is enhanced in the renal tubular epithelium of animals subjected to ischemic reperfusion injury (IRI).


CONCLUSIONS
The role of PRMT5 in the regulation of mitosis, its induction in renal epithelial cells undergoing tubule formation in vitro and its expression in the tubules of the kidneys subjected to IRI suggest that it functions in the regulation of cell growth and differentiation during tubule formation and regeneration.
Braun
Michael C
MC
Division of Pediatric Nephrology and Hypertension, Institute for Molecular Medicine, Houston, TX, USA.
Kelly
Caitlin N
CN
Prada
Anne E
AE
Mishra
Jaya
J
Chand
Deepa
D
Devarajan
Prasad
P
Zahedi
Kamyar
K
D002478:Cells, Cultured; D006801:Humans; D007668:Kidney / Q000166:cytology* / Q000378:metabolism*; D007684:Kidney Tubules / Q000254:growth & development / Q000378:metabolism*; D011496:Protein Methyltransferases / Q000096:biosynthesis* / Q000235:genetics; D011484:Protein-Arginine N-Methyltransferases; D012333:RNA, Messenger / Q000032:analysis; D015427:Reperfusion Injury / Q000378:metabolism*; D019459:Urothelium / Q000166:cytology / Q000378:metabolism
D012333:RNA, Messenger; D011496:Protein Methyltransferases; C400529:PRMT5 protein, human; D011484:Protein-Arginine N-Methyltransferases