Ternatin, a flavonoid, prevents cyclophosphamide and ifosfamide-induced hemorrhagic cystitis in rats.
To compare the classical uroprotective efficacy of mesna (2-mercaptoethanesulfonic acid) with ternatin (flavonoid isolated from Egletes viscosa Less.) in cyclophosphamide (CYP) and ifosfamide (IFS) induced hemorrhagic cystitis (HC). Male Wistar rats received an intraperitoneal injection of saline, CYP or IFS and were treated with saline or mesna, 5 min before, 4 and 8 h after CYP or IFS administration. In other animals, 1, 2 or 3 doses of mesna were replaced with ternatin or 3 doses of mesna were replaced with dimethylsulphoxide (DMSO), ternatin diluent. In an additional group, the last 2 doses of mesna were replaced with saline. HC was evaluated 24 h after CYP or IFS administration. CYP or IFS treatment induced marked changes in macroscopic and microscopic evaluation and in bladder wet weight (BWW), and these alterations were significantly inhibited by treatment with 3 doses of mesna, as well as by the replacement of 1 or 2 doses of mesna with ternatin. The replacement of 2 doses of mesna with saline or all doses of mesna with ternatin or DMSO did not prevent HC. In conclusion, the replacement of 1 or 2 doses of mesna with ternatin efficiently blocked CYP- or IFS-induced HC, however mesna is necessary for initial uroprotection.
Vieira
Milena Morais
MM
Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Brazil.
Macêdo
Francisco Yuri B
FY
Filho
José Nelson Belarmino
JN
Costa
Ana Carolina L V
AC
Cunha
Adriano N
AN
Silveira
Edilberto R
ER
Brito
Gerly Anne C
GA
Ribeiro
Ronaldo A
RA
D000818:Animals; D019659:Asteraceae; D003520:Cyclophosphamide; D003556:Cystitis / Q000139:chemically induced / Q000517:prevention & control*; D005419:Flavonoids / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D035264:Flowers; D006470:Hemorrhage / Q000139:chemically induced / Q000517:prevention & control; D007069:Ifosfamide; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D020011:Protective Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D001743:Urinary Bladder / Q000187:drug effects / Q000473:pathology
D005419:Flavonoids; D010936:Plant Extracts; D020011:Protective Agents; C051080:ternatin (flavonoid); D003520:Cyclophosphamide; D007069:Ifosfamide