title: Purification, crystallization and preliminary X-ray analysis of uridine phosphorylase from Salmonella typhimurium.
abstract: The structural udp gene encoding uridine phosphorylase (UPh) was cloned from the Salmonella typhimurium chromosome and overexpressed in Escherichia coli cells. S. typhimurium UPh (StUPh) was purified to apparent homogeneity and crystallized. The primary structure of StUPh has high homology to the UPh from E. coli, but the enzymes differ substantially in substrate specificity and sensitivity to the polarity of the medium. Single crystals of StUPh were grown using hanging-drop vapor diffusion with PEG 8000 as the precipitant. X-ray diffraction data were collected to 2.9 A resolution. Preliminary analysis of the diffraction data indicated that the crystal belonged to space group P6(1(5)), with unit-cell parameters a = 92.3, c = 267.5 A. The solvent content is 37.7% assuming the presence of one StUPh hexamer per asymmetric unit.
authors.affiliation: Institute of Crystallography, Russian Academy of Sciences, Russia, and Institute of Protein Research, Russian Academy of Sciences, Russia.
authors.forename: Mariya V
authors.initials: MV
authors.lastname: Dontsova
authors.forename: Yulia A
authors.initials: YA
authors.lastname: Savochkina
authors.forename: Azat G
authors.initials: AG
authors.lastname: Gabdoulkhakov
authors.forename: Sergey N
authors.initials: SN
authors.lastname: Baidakov
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Lyashenko
authors.forename: Maria
authors.initials: M
authors.lastname: Zolotukhina
authors.forename: Liubov
authors.initials: L
authors.lastname: Errais Lopes
authors.forename: Mariya B
authors.initials: MB
authors.lastname: Garber
authors.forename: Ekaterina Yu
authors.initials: EY
authors.lastname: Morgunova
authors.forename: Stanislav V
authors.initials: SV
authors.lastname: Nikonov
authors.forename: Alexandr S
authors.initials: AS
authors.lastname: Mironov
authors.forename: Steven E
authors.initials: SE
authors.lastname: Ealick
authors.forename: Al 'Bert M
authors.initials: A'
authors.lastname: Mikhailov
mesh_terms: D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D012486:Salmonella typhimurium / Q000201:enzymology*; D014543:Uridine Phosphorylase / Q000737:chemistry*
chemical_list: D014543:Uridine Phosphorylase