A novel antioxidant-inhibited dexamethasone-mediated and caspase-3-independent muscle cell death.
Dexamethasone (Dex)-mediated cell death is associated with repression of survival factors (AP-1, c-myc, NF-kappaB). Dex suppressed the activity of genes encoding antioxidant enzymes leading to impaired viability and apoptotic cell death. These findings suggest that reactive oxygen species inhibit protein synthesis and amplify m-calpain-dependent proteolysis. The events that led to death of L6 muscle cells were most likely triggered by Dex-mediated repression of antioxidative defenses on the genomic level.
Orzechowski
Arkadiusz
A
Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw Agricultural University, Warsaw, Poland. orzechowski@alpha.sggw.waw.pl
Jank
Michal
M
Gajkowska
Barbara
B
Sadkowski
Tomasz
T
Godlewskia
Michal Marek
MM
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism*; D016923:Cell Death / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003907:Dexamethasone / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D018482:Muscle, Skeletal / Q000166:cytology* / Q000187:drug effects
D000975:Antioxidants; D003907:Dexamethasone; D053148:Caspase 3; D020169:Caspases