Analysis of protein absorbed by LDL column (Liposorber) with special reference to complement component factor D.
BACKGROUND
A dextran sulfate column (Liposorber) has been developed and proven effective in LDL apheresis for removing LDL from patients with hyperlipemia. Chronic renal failure (CRF) patients with arteriosclerosis obliterans (ASO) treated with Liposorber have been shown good results.


METHODS
We analyzed proteins absorbed by Liposorber in CRF patients with ASO by means of 2-dimensional electrophoresis and found some noteworthy protein spots. One of these proteins was purified by Sephacryl S-100 column and DEAE Sepharose fast flow column chromatography.


RESULTS
The purified protein was finally identified as complement factor D by Western blotting. The average of recovery amount from three experiments was 6.5 mg. The total recovered amount of factor D was calculated to be about 21.7% out of the estimated amount of factor D in the plasma of CRF patients with ASO receiving LDL apheresis using Liposorber.


CONCLUSIONS
These results indicate that Liposorber absorbs factor D remarkably well.
Oda
Osamu
O
The Biodynamics Research Institute, 1-3-2 Tamamizu-cho, Mizuho-ku, Nagoya 467-8633, Japan.
Nagaya
Takashi
T
Ogawa
Hiroshi
H
D001162:Arteriosclerosis Obliterans / Q000097:blood / Q000150:complications / Q000628:therapy; D001781:Blood Component Removal; D015153:Blotting, Western; D011416:Complement Factor D / Q000032:analysis*; D016264:Dextran Sulfate; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood / Q000150:complications / Q000628:therapy; D007676:Kidney Failure, Chronic / Q000097:blood / Q000150:complications / Q000628:therapy; D008077:Lipoproteins, LDL / Q000097:blood / Q000302:isolation & purification*; D006435:Renal Dialysis
D008077:Lipoproteins, LDL; D016264:Dextran Sulfate; C493488:CFD protein, human; D011416:Complement Factor D