title: [Determination of glycosides in traditional Chinese medicine liu-wei di-huang by RP-HPLC].
abstract: OBJECTIVE
  To establish a RP-HPLC method for determination of glycosides in Traditional Chinese Medicine Liu-wei Di-huang.
  
  
  METHODS
  The samples were analyzed on an ODS column at 30 degrees C, with mobile phase of methanol/water (33:67) at flow rate 1.0 mL.min and detection at wavelength of 236 nm.
  
  
  RESULTS
  Three major components reached base-line separation and were identified to be mononiside, loganin, paeoniflorin. Respectively for the three components, linear correlations were found between peak areas and concentrations in the ranges of 7.4-60, 7.7-62 mg.L-1 and 8.5-68 mg.L-1, and the recoveries were 98.8%, 98.3%, 99.6%.
  
  
  CONCLUSIONS
  The established method is proved to be suitable for simultaneous quantification of three major glycosidic components in Liuwei Dihuang decoction and can be used for evaluation of the quality of Liuwei Dihuang preparations.
authors.affiliation: Academy of Military Medical Sciences, Institute of Pharmacology and Toxicology, Bingjing 100850, China.
authors.forename: Shan-li
authors.initials: SL
authors.lastname: Xiong
authors.forename: Yi-min
authors.initials: YM
authors.lastname: Zhao
authors.forename: Xin-hui
authors.initials: XH
authors.lastname: Luan
authors.forename: Shan-yi
authors.initials: SY
authors.lastname: Qiao
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.forename: Feng-xia
authors.initials: FX
authors.lastname: Ren
authors.forename: Ji-fen
authors.initials: JF
authors.lastname: Guo
authors.forename: Yong-xiang
authors.initials: YX
authors.lastname: Zhang
mesh_terms: D001565:Benzoates / Q000032:analysis*; D001952:Bridged-Ring Compounds / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D029768:Cornus / Q000737:chemistry; D027742:Dioscorea / Q000737:chemistry; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis*; D039823:Iridoids / Q000032:analysis*; D039821:Monoterpenes; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D032267:Rehmannia / Q000737:chemistry; D006027:Glycosides
chemical_list: D001565:Benzoates; D001952:Bridged-Ring Compounds; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039823:Iridoids; D039821:Monoterpenes; C042579:Liuwei Dihuang Decoction; C015423:peoniflorin; D006027:Glycosides; C059516:loganin