[Experimental studies on the apoptosis of HL-60 cells induced by Brucea javanica oil emulsion].
OBJECTIVE
To investigate the inducing effect of Brucea javanica on the apoptpsis of HL-60 cells.


METHODS
HL-60 cells were treated with Brucea javanica 1:100, 1:40, 1:20 (v/v) respectively for 6 h and DNA agarose gel electrophoresis, flow cytometry, fluorescence microscope, electron microscope were used to observe the apoptosis inducing effect of Brucea javanica.


RESULTS
DNA ladder was seen in the 1:40 group. The apoptosis cell percentages of 1:40 and 1:20 group were 86.8% and 97% respectively. Cells of 1:40 group showed obvious apoptosis character under fluorescence microscope. Cells were induced apoptosis in 1:20 and 1:40 Brucea javanica under electron microscope.


CONCLUSIONS
1:20 and 1:40 Brucea Javanica showed obvious apoptosis inducing effect of HL-60.
Wang
Fang
F
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China. Wangfangpumc@hotmail.com
Cao
Yu
Y
Liu
Hong-yan
HY
Fu
Zhao-di
ZD
Han
Rui
R
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D032269:Brucea / Q000737:chemistry; D018922:HL-60 Cells; D006801:Humans; D010938:Plant Oils / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry
D000972:Antineoplastic Agents, Phytogenic; D010938:Plant Oils