title: Delineation of a minimal region of deletion at 6q16.3 in follicular lymphoma and construction of a bacterial artificial chromosome contig spanning a 6-megabase region of 6q16-q21.
abstract: Regional deletions of 6q are frequent karyotypic alterations in malignant lymphoma and are associated with an adverse clinical outcome. One such region of recurrent deletion is 6q16-q21; however, the specific genes affected have not been identified. Our objective in this study was to identify cases with deletion of 6q16-q21 in follicular lymphoma and to define a minimal region of deletion. A physical map of 6q16.2-q21 was constructed using map information from both sequence-based and bacterial artificial chromosome (BAC) fingerprint-based maps. Forty-three BAC clones spanning a 6-Mb region of 6q16.2-q21 were identified and obtained from the RP-11 library. Selected BACs were fluorescence-labeled and hybridized to a series of 34 follicular lymphomas with a regional 6q deletion detected by G banding. Twenty-four cases with deletion of the 6q16.3 region were detected. A minimal deletion of 2.3 Mb was defined. Our study has identified a limited region of deletion of 6q16.3 that may implicate four known genes in follicular lymphoma and possibly in other cancers. A BAC contig spanning a 6-Mb region has been anchored to the 6q16.2-q21 region. This map represents a useful resource for gene identification in this region, not only in lymphoma but also in other neoplasms with 6q alterations.
authors.affiliation: British Columbia Cancer Agency, Vancouver, BC, Canada. ljhender@bccrc.ca
authors.forename: Laura-Jane
authors.initials: LJ
authors.lastname: Henderson
authors.forename: Ichiro
authors.initials: I
authors.lastname: Okamoto
authors.forename: Valia S
authors.initials: VS
authors.lastname: Lestou
authors.forename: Olga
authors.initials: O
authors.lastname: Ludkovski
authors.forename: Marc
authors.initials: M
authors.lastname: Robichaud
authors.forename: Mukesh
authors.initials: M
authors.lastname: Chhanabhai
authors.forename: Randy D
authors.initials: RD
authors.lastname: Gascoyne
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Klasa
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Connors
authors.forename: Marco A
authors.initials: MA
authors.lastname: Marra
authors.forename: Douglas E
authors.initials: DE
authors.lastname: Horsman
authors.forename: Wan L
authors.initials: WL
authors.lastname: Lam
mesh_terms: D002872:Chromosome Deletion; D002874:Chromosome Mapping / Q000379:methods*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D020451:Contig Mapping / Q000379:methods*; D005260:Female; D006801:Humans; D018404:Isochromosomes / Q000235:genetics; D007621:Karyotyping; D008224:Lymphoma, Follicular / Q000235:genetics*; D008297:Male