title: Effect of D-lactate on the physiological activity of the ArcB sensor kinase in Escherichia coli.
abstract: The Arc two-component system, comprising the ArcB sensor kinase and the ArcA response regulator, modulates the expression of numerous genes in response to the respiratory growth conditions. Under anoxic growth conditions ArcB autophosphorylates and transphosphorylates ArcA, which in turn represses or activates its target operons. The anaerobic metabolite D-lactate has been shown to stimulate the in vitro autophosphorylating activity of ArcB. In this study, the in vivo effect of D-lactate on the kinase activity of ArcB was assessed. The results demonstrate that D-lactate does not act as a direct signal for activation of ArcB, as previously proposed, but acts as a physiologically significant effector that amplifies ArcB kinase activity.
authors.affiliation: Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 04510 Mexico City, Mexico.
authors.forename: Claudia
authors.initials: C
authors.lastname: Rodriguez
authors.forename: Ohsuk
authors.initials: O
authors.lastname: Kwon
authors.forename: Dimitris
authors.initials: D
authors.lastname: Georgellis
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D019344:Lactic Acid / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D003470:Culture Media; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D019344:Lactic Acid; D011494:Protein Kinases; C065271:arcB protein, E coli