title: Functional microdomains of glycolipids with partially fluorinated membrane anchors: impact on cell adhesion.
abstract: Functional microdomains of glycolipids were designed by mixing neoglycolipids with partially fluorinated alkyl (F-alkyl) chains and matrix lipids with alkyl chains. Fluorescence images of the mixed lipid monolayers at the air-water interface demonstrated that it is possible to control both size and distribution of the microdomains by means of the strong demixing of alkyl and F-alkyl membrane anchors, while the carbohydrate head groups seemed to play a rather minor role. These microdomains in monolayers could be transferred onto hydrophobized substrates and subjected to experiments in a dynamic flow chamber. The results obtained here clearly indicated that the dynamic adhesion of Chinese hamster ovarial cells expressing E-selectin (CHO-E cells) on a lipid monolayer containing microdomains of sialyl Lewisx (sLex) can be both enhanced and reduced by controlled demixing of ligands and matrices. Moreover, the same clusters of sLex could also be formed in giant lipid vesicles, which can be used as a model cell that locally expresses biospecific functions.
authors.affiliation: Universität Konstanz, Fach M 725 78457 Konstanz, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Gege
authors.forename: Matthias F
authors.initials: MF
authors.lastname: Schneider
authors.forename: Gabriele
authors.initials: G
authors.lastname: Schumacher
authors.forename: Laurent
authors.initials: L
authors.lastname: Limozin
authors.forename: Ulrich
authors.initials: U
authors.lastname: Rothe
authors.forename: Gerd
authors.initials: G
authors.lastname: Bendas
authors.forename: Motomu
authors.initials: M
authors.lastname: Tanaka
authors.forename: Richard R
authors.initials: RR
authors.lastname: Schmidt
mesh_terms: D000388:Air; D000818:Animals; D016466:CHO Cells; D002240:Carbohydrate Sequence; D002448:Cell Adhesion / Q000502:physiology*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D006224:Cricetinae; D019040:E-Selectin / Q000235:genetics / Q000378:metabolism; D004730:Endothelium, Vascular / Q000502:physiology; D006017:Glycolipids / Q000138:chemical synthesis / Q000378:metabolism*; D006845:Hydrocarbons, Fluorinated / Q000138:chemical synthesis; D057927:Hydrophobic and Hydrophilic Interactions; D008024:Ligands; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism / Q000494:pharmacology; D000080506:Sialyl Lewis X Antigen; D014867:Water
chemical_list: D019040:E-Selectin; D006017:Glycolipids; D006845:Hydrocarbons, Fluorinated; D008024:Ligands; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; D014867:Water