Development of canine nephrotic syndrome model.
To develop an experimental animal model for immune-mediated glomerulonephritis and nephrotic syndrome, nine healthy dogs were sensitized by intravenous injection with 1 microg of endotoxin and 5 mg of native bovine serum albumin. After 1 week, 120 mg of cationized bovine serum albumin was injected intravenously 5 times a week. Among nine dogs, five dogs were confirmed as having developed glomerulonephritis and nephrotic syndrome by increase of urine protein-to-creatinine ratio (>1.0), hypoalbuminemia (<1.5 g/dl), hypercholesterolemia (>240 mg/dl), and edema. This model should be useful for studying immune-mediated glomerulonephritis and nephrotic syndrome.
Choi
Eun-Wha
EW
Department of Veterinary Clinical Pathology, College of Veterinary Medicine, Seoul National University, Kwanak-gu, Korea.
Lee
Chang-Woo
CW
D000818:Animals; D003401:Creatine / Q000652:urine; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D005921:Glomerulonephritis / Q000139:chemically induced*; D007150:Immunohistochemistry; D007668:Kidney / Q000473:pathology; D008297:Male; D009404:Nephrotic Syndrome / Q000139:chemically induced*; D012710:Serum Albumin, Bovine
D012710:Serum Albumin, Bovine; D003401:Creatine