Inhibitory phlorotannins from the edible brown alga Ecklonia stolonifera on total reactive oxygen species (ROS) generation.
Reactive oxygen species (ROS) play an important role in the pathogenesis of many human degenerative diseases such as cancer, aging, arteriosclerosis, and rheumatism. Much attention has been focused on the development of safe and effective antioxidants. To discover sources of antioxidative activity in marine algae, extracts from 17 kinds of seaweed were screened for their inhibitory effect on total ROS generation in kidney homogenate using 2',7'-dichlorofluorescein diacetate (DCFH-DA). ROS inhibition was seen in three species: Ulva pertusa, Symphyocladia latiuscula, and Ecklonia stolonifera. At a final concentration of 25 microg/mL, U. pertusa inhibited 85.65+/-20.28% of total ROS generation, S. latiscula caused 50.63+/-0.09% inhibitory, and the Ecklonia species was 44.30+/-7.33% inhibition. E. stolonifera Okamura (Laminariaceae), which belongs to the brown algae, has been further investigated because it is commonly used as a foodstuff in Korea. Five compounds, phloroglucinol (1), eckstolonol (2), eckol (3), phlorofucofuroeckol A (4), and dieckol (5), isolated from the ethyl acetate soluble fraction of the methanolic extract of E. stolonifera inhibited total ROS generation.
Kang
Hye Sook
HS
Faculty of Food Science and Biotechnology, Pukyong National University, Busan 608-737, Korea.
Chung
Hae Young
HY
Kim
Ji Young
JY
Son
Byeng Wha
BW
Jung
Hyun Ah
HA
Choi
Jae Sue
JS
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002855:Chromatography, Thin Layer; D004147:Dioxins / Q000302:isolation & purification / Q000494:pharmacology*; D005452:Fluoresceins; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D000459:Phaeophyceae / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D000975:Antioxidants; D004147:Dioxins; D005452:Fluoresceins; D017382:Reactive Oxygen Species; C037631:2',7'-dichlorofluorescein