[Purification and characterization of lipases from wheat germ].
A method of isolation and purification of lipase (EC 3.1.1.3) from the germ of wheat (Triticum aestivum) is described. Electrophoretically homogeneous preparation of the enzyme (specific activity, 622.5 x x 10(-3) mumol/min per mg protein) was obtained after purification in 61 times. The molecular weight of the enzyme, determined by gel chromatography, was 143 +/- 2 kDa. The optimal conditions for the enzyme were 37 degrees and pH 8.0. Homogeneous preparation of the lipase exhibited high thermal stability: over 20% of original activity was retained after incubation of the preparation at high temperatures (60-90 degrees) for 1 h at pH 8.0.
Kapranchikov
V S
VS
Voronezh State Technological Academy, pr. Revolyutsii 19, Voronezh, 394017 Russia.
Zherebtsov
N A
NA
Popova
T N
TN
D002850:Chromatography, Gel; D007526:Isoelectric Point; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D008970:Molecular Weight; D012639:Seeds / Q000201:enzymology*; D014908:Triticum / Q000196:embryology / Q000201:enzymology*
D008049:Lipase