title: ProteinChip array-based amyloid beta assays.
abstract: Amyloid-beta (A beta) fragments are found in plaques of patients with Alzheimers. Three secretases cleave the amyloid precursor protein, producing multiple A beta fragments that accumulate in the brain and fluids of patients with Alzheimers. A beta peptides are difficult to detect using standard methods because of their small size and multiple isoforms. However, multiple peptide fragments can be detected using a single ProteinChip Array-Based assay. Specific antibodies recognizing various amyloid epitopes are immobilized on a ProteinChip Array. Crude samples, such as tissue lysates, serum, cerebral spinal fluid (CSF), or cell culture media, are applied to the antibody-coated arrays. A beta peptides are specifically retained by the antibody, whereas other sample components are removed by washing. The multiple peptide fragments are detected by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS), which can easily resolve the different fragments because of the corresponding changes in peptide mass.
authors.affiliation: Ciphergen Biosystems, Inc, Fremont, CA, USA.
authors.forename: Lisa E
authors.initials: LE
authors.lastname: Bradbury
authors.forename: James F
authors.initials: JF
authors.lastname: LeBlanc
authors.forename: Diane B
authors.initials: DB
authors.lastname: McCarthy
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism; D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000032:analysis*; D016564:Amyloid beta-Protein Precursor / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000378:metabolism; D001923:Brain Chemistry; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D040081:Protein Array Analysis / Q000379:methods*; D012015:Reference Standards; D016415:Sequence Alignment
chemical_list: D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D000906:Antibodies