Characterization of high-salt and high-fat diets on cardiac and vascular function in mice.
This study compared two established dietary formulations, high salt and high fat-high carbohydrate, separately or in combination on the induction cardiovascular dysfunction. One-month-old C57BL/6J mice were fed one of the following diets for 3 mo: (1) control diet consisting of a high fat-high sim-ple carbohydrate (HFHSC); (2) 8% NaCl diet (HS); or (3) HFHSC diet supplemented with 1% NaCl (HFHS). After 3 mo, the HFHSC mice demonstrated significantly increased end-diastolic volume and end-systolic volume, specifically increases of 35% and 78%, respectively (p < 0.01) and a reduction of ventricular stiffness by 27% (p = 0.015). The HS mice exhibited arterial hyper-tension with an increase of 33% in maximum end-systolic pressure (p =.024) and a decrease of 44% in arterial elastance (p = 0.020), corroborated by an increase in the heart weight to body weight ratios (p = 0.002) and vascular types I and III collagen (p = 0.03 and p = 0.0008, respectively). The HFHS group revealed a striking response of 230% to the alpha1-adrenergic challenge (p = 0.00034). These data suggest that the HFHSC diet causes dilated cardio-myopathy, whereas the HS diet produces arterial hypertension and the HFHS diet causes a vascular dysfunctional state that was highly responsive to alpha-adrenergic stimulation.
Yu
Qianli
Q
College of Public Health, School of Medicine, Health Promotion Science Division, University of Arizona, Tucson, AZ 85724, USA.
Larson
Douglas F
DF
Slayback
Denise
D
Lundeen
Tamara F
TF
Baxter
Jeffrey H
JH
Watson
Ronald R
RR
D000818:Animals; D001158:Arteries / Q000187:drug effects; D003094:Collagen / Q000096:biosynthesis / Q000235:genetics; D004032:Diet; D004040:Dietary Carbohydrates / Q000494:pharmacology; D004041:Dietary Fats / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects*; D006331:Heart Diseases / Q000209:etiology / Q000503:physiopathology; D006439:Hemodynamics / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017673:Sodium Chloride, Dietary / Q000494:pharmacology*; D016277:Ventricular Function, Left / Q000187:drug effects
D004040:Dietary Carbohydrates; D004041:Dietary Fats; D012333:RNA, Messenger; D017673:Sodium Chloride, Dietary; D003094:Collagen