Evaluation of antibodies and microarray coatings as a prerequisite for the generation of optimized antibody microarrays.
Antibody microarrays are becoming a major tool for the parallel analysis of complex samples. So far, many efforts have been made to increase the complexity and sensitivity of antibody microarrays. In contrast to enzyme-linked immunosorbent assay (ELISA) experiments, not all antibodies remain functional in the microarray format. Sensitivity is very much dependent on the type of coating and its application. The method described in this chapter is a quick and reliable method that has been very useful in determining the functionality of antibodies and the suitability of coatings for antibody microarrays. At the same time, a detailed description of how to prepare an inexpensive and highly efficient antibody microarray surface is given.
Angenendt
Philipp
P
Department Lehrach, Max Planck Institute for Molecular Genetics, Berlin, Germany.
Glökler
Jörn
J
D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000378:metabolism*; D000941:Antigens / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005456:Fluorescent Dyes / Q000378:metabolism; D011107:Polylysine / Q000378:metabolism; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D012680:Sensitivity and Specificity; D013499:Surface Properties
D000906:Antibodies; D000941:Antigens; D005456:Fluorescent Dyes; D011506:Proteins; D011107:Polylysine