Cloning and characterization of two novel lipid transfer protein I genes in Vigna radiata.
Two full-length lipid transfer protein (LTP) cDNAs were isolated from mungbean (Vigna radiata) and designated Vrltp1 and Vrltp2. The deduced amino acid sequences contain the two highly conserved pentapeptides characteristic of plant LTPIs suggesting these Vrltps belong to the LTPI gene family. Vrltp1 mRNA was detected in developing seeds, but Vrltp2 mRNA was not. Within the vegetative tissues, the Vrltp1 and Vrltp2 mRNAs were present only in leaves and stems, but not root tips. Salt and dehydration stresses and exogenous abscisic acid (ABA) treatments resulted in increased mRNA levels of both Vrltps in leaves. We suggest that these unique Vrltps are specific to growing shoot tissues, and may play an important role in plant acclimation to water stress.
Liu
Kun-Hsiang
KH
Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan 30013, Republic of China.
Lin
Tsai-Yun
TY
D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D003681:Dehydration; D007887:Fabaceae / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins; D012492:Salts / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D052179:Antigens, Plant; D002352:Carrier Proteins; D010940:Plant Proteins; D012492:Salts; C511920:lipid transfer proteins, plant