title: Translocation (11;13)(q23;q14) as the sole abnormality in a childhood de novo acute myelocytic leukemia.
abstract: We report a case of childhood de novo acute myelocytic leukemia (AML) with hyperleukocytosis with monoblastic features and deranged hemostasic function. G-band karyotyping demonstrated a previously unreported t(11;13)(q23;q14) in metaphase preparations from a fluorodeoxyuridine synchronized 1-day culture of leukophoresed cells. Multicolor fluorescence in situ hybridization revealed no cryptic rearrangements except for the translocation. Reverse transcriptase polymerase chain reaction showed no concomitant positivity of AML1/ETO, BCR/ABL, PML/RARA, and CBFbeta/MYH11 resulting from t(8;21)(q22;q22), t(9;22)(q34;q11), t(15;17)(q22;q11), and inv(16) (p13q22), respectively. This report of childhood de novo AML harboring t(11;13)(q23;q14) as the sole cytogenetic abnormality provides more data on the leukemogenesis of de novo AML with a 11q23 rearrangement.
authors.affiliation: Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Ngan Shing Street, Hong Kong, China. tsangks@cuhk.edu.hk
authors.forename: Kam Sze
authors.initials: KS
authors.lastname: Tsang
authors.forename: Chi Kong
authors.initials: CK
authors.lastname: Li
authors.forename: Tai Tap
authors.initials: TT
authors.lastname: Lau
authors.forename: Angela Pui Yan
authors.initials: AP
authors.lastname: Wong
authors.forename: Yonna
authors.initials: Y
authors.lastname: Leung
authors.forename: Margaret Heung Ling
authors.initials: MH
authors.lastname: Ng
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D002648:Child; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics*; D005260:Female; D015321:Gene Rearrangement; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014178:Translocation, Genetic
chemical_list: D014408:Biomarkers, Tumor