Antibiotic-mediated antagonism leads to a bacterial game of rock-paper-scissors in vivo.
Colicins are narrow-spectrum antibiotics produced by and active against Escherichia coli and its close relatives. Colicin-producing strains cannot coexist with sensitive or resistant strains in a well-mixed culture, yet all three phenotypes are recovered in natural populations. Recent in vitro results conclude that strain diversity can be promoted by colicin production in a spatially structured, non-transitive interaction, as in the classic non-transitive model rock-paper-scissors (RPS). In the colicin version of the RPS model, strains that produce colicins (C) kill sensitive (S) strains, which outcompete resistant (R) strains, which outcompete C strains. Pairwise in vitro competitions between these three strains are resolved in a predictable order (C beats S, S beats R, and R beats C), but the complete system of three strains presents the opportunity for dynamic equilibrium. Here we provide conclusive evidence of an in vivo antagonistic role for colicins and show that colicins (and potentially other bacteriocins) may promote, rather than eliminate, microbial diversity in the environment.
Kirkup
Benjamin C
BC
Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut 06511, USA. bckirkup@post.harvard.edu
Riley
Margaret A
MA
D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D003087:Colicins / Q000378:metabolism*; D003106:Colon / Q000382:microbiology; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D005243:Feces / Q000382:microbiology; D005716:Game Theory; D006799:Housing, Animal; D008297:Male; D051379:Mice; D008954:Models, Biological; D013307:Streptomycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D003087:Colicins; D013307:Streptomycin