title: Redox regulation of PTEN and protein tyrosine phosphatases in H(2)O(2) mediated cell signaling.
abstract: Protein tyrosine phosphatase (PTP) is a family of enzymes important for regulating cellular phosphorylation state. The oxidation and consequent inactivation of several PTPs by H(2)O(2) are well demonstrated. It is also shown that recovery of enzymatic activity depends on the availability of cellular reductants. Among these redox-regulated PTPs, PTEN, Cdc25 and low molecular weight PTP are known to form a disulfide bond between two cysteines, one in the active site and the other nearby, during oxidation by H(2)O(2). The disulfide bond likely confers efficiency in the redox regulation of the PTPs and protects cysteine-sulfenic acid of PTPs from further oxidation. In this review, through a comparative analysis of the oxidation process of Yap1 and PTPs, we propose the mechanism of disulfide bond formation in the PTPs.
authors.affiliation: Center for Cell Signaling Research, Department of Biological Sciences, Ewha Women's University, Seoul, South Korea.
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Cho
authors.forename: Chang-Hun
authors.initials: CH
authors.lastname: Lee
authors.forename: Younghee
authors.initials: Y
authors.lastname: Ahn
authors.forename: Hyunjung
authors.initials: H
authors.lastname: Kim
authors.forename: Hoeon
authors.initials: H
authors.lastname: Kim
authors.forename: Chi-Young
authors.initials: CY
authors.lastname: Ahn
authors.forename: Kap-Seok
authors.initials: KS
authors.lastname: Yang
authors.forename: Seung-Rock
authors.initials: SR
authors.lastname: Lee
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002477:Cells / Q000378:metabolism; D004220:Disulfides / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008954:Models, Biological; D008958:Models, Molecular; D016877:Oxidants / Q000633:toxicity*; D010084:Oxidation-Reduction; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D015398:Signal Transduction; D014157:Transcription Factors; D000091102:YAP-Signaling Proteins; D000099202:PTEN-Induced Putative Kinase
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D004220:Disulfides; D006861:Hydrogen Peroxide; D016877:Oxidants; D010750:Phosphoproteins; D011494:Protein Kinases; D017027:Protein Tyrosine Phosphatases; D014157:Transcription Factors; D000091102:YAP-Signaling Proteins; D000099202:PTEN-Induced Putative Kinase; C088374:YAP1 protein, human