The impact of manipulations with cytoplasmically inherited factors on nuclear transmission and degradation in yeast heterokaryons.
Heterokaryotic zygotes in yeast provide a unique possibility to study the survival and transmission of two genetically diverse nuclei in one cell. Using partial pedigree analysis, we show that various treatments used to change cytoplasmic hereditary determinants can essentially affect nuclear transmission in yeast heterokaryons. This includes choice of nucleus to enter the first bud and incidence of various classes of mother/daughter pairs demonstrating nuclear degradation patterns in heterokaryotic zygotes. These treatments include guanidine hydrochloride, a prion-curing agent, ethidium bromide, an agent causing elimination of mitochondrial DNA, and cytoplasm replacement by cytoduction, which leads to mtDNA replacement and transfer of some other cytoplasmically inherited determinants. The genetic and cytological evidence obtained favors prion involvement in nuclear transmission and suggests apoptotic features in nuclear degradation in yeast heterokaryotic zygotes.
Nevzglyadova
Olga V
OV
Institute of Cytology, Tikhoretsky Ave. 4, 194064, St Petersburg, Russia. oneva@mail.cytspb.rssi.ru
Artyomov
Alexey V
AV
Mikhailova
Ekaterina V
EV
Soidla
Tonu R
TR
D017209:Apoptosis; D002467:Cell Nucleus / Q000737:chemistry* / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D004272:DNA, Mitochondrial / Q000737:chemistry; D004996:Ethidium / Q000494:pharmacology; D005656:Fungal Proteins / Q000737:chemistry; D005821:Genetic Techniques; D019791:Guanidine / Q000737:chemistry / Q000494:pharmacology; D006238:Haploidy; D007364:Intercalating Agents / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D010641:Phenotype; D011328:Prions / Q000737:chemistry; D017382:Reactive Oxygen Species; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000502:physiology*; D015053:Zygote / Q000737:chemistry / Q000378:metabolism
D004272:DNA, Mitochondrial; D005656:Fungal Proteins; D007364:Intercalating Agents; D011328:Prions; D017382:Reactive Oxygen Species; D004996:Ethidium; D019791:Guanidine