title: Determination of 1-deoxynojirimycin in mulberry leaves using hydrophilic interaction chromatography with evaporative light scattering detection.
abstract: A simple and rapid method for determining 1-deoxynojirimycin (DNJ), a potent glucosidase inihibitor present in mulberry leaves (Morus alba and Morus bombysis), by high-performance liquid chromatography coupled to an evaporative light scattering detector (ELSD) has been developed. DNJ was separated from an extract of mulberry leaves on a TSKgel Amide-80 column, which is a representative column for hydrophilic interaction chromatography. During postcolumn detection, DNJ was detected by ELSD and concurrently identified by mass spectrometry. The detection limit was 100 ng. This method is sufficiently sensitive for determining DNJ in mulberry leaves and other related products.
authors.affiliation: National Agricultural Research Center for Tohoku Region, Fukushima 960-2156, Japan. kmr@affrc.go.jp
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kimura
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Yuko
authors.initials: Y
authors.lastname: Saito
authors.forename: Kenji
authors.initials: K
authors.lastname: Yamagishi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Suzuki
authors.forename: Kohji
authors.initials: K
authors.lastname: Yamaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shinmoto
authors.forename: Teruo
authors.initials: T
authors.lastname: Miyazawa
mesh_terms: D017485:1-Deoxynojirimycin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008027:Light; D031625:Morus / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D012542:Scattering, Radiation
chemical_list: D017485:1-Deoxynojirimycin