Fabrication and application of G protein-coupled receptor microarrays.
The increased number of drug targets and compounds demands novel high-throughput screening technologies that could be used for parallel analysis of many genes and proteins. Protein microarrays are evolving promising technologies for the parallel analysis of many proteins with respect to their abundance, location, modifications, and interactions with other biological and chemical molecules. This chapter specifically describes the fabrication of G protein-coupled receptor (GPCR) microarrays, a unique subset of protein microarrays, using contact-pin printing technology. The bioassays and potential applications of GPCR microarrays for the determination of compound affinities and potencies are also included.
Fang
Ye
Y
Science and Technology Division, Biochemical Technologies, Corning, Inc, Corning, NY, USA.
Webb
Brian
B
Hong
Yulong
Y
Ferrie
Ann
A
Lai
Fang
F
Frutos
Anthony G
AG
Lahiri
Joydeep
J
D000818:Animals; D002021:Buffers; D006801:Humans; D008024:Ligands; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011485:Protein Binding; D043562:Receptors, G-Protein-Coupled / Q000032:analysis*
D002021:Buffers; D008024:Ligands; D043562:Receptors, G-Protein-Coupled