Optimal conditions for accumulation of bioavailable iron in Saccharomyces cerevisiae cells.
Growth and proliferation of brewer's yeast Saccharomyces cerevisiae in the presence of different Fe2+ levels was studied with the aim of finding optimal conditions for a maximum accumulation of bioavailable iron bound to constituents of yeast cells. The results demonstrated that iron stimulates growth and proliferation only under conditions of intensive aeration. Iron accumulation and the effect of aeration were examined in the presence 3.6 microM - 7.2 mM Fe2+, while its content in the cells after 20 h cultivation was determined by atomic absorption spectrophotometry. Control cultures were grown in no iron added medium. Further experiments revealed that iron concentrations ranging from 3.6 microM to 3.6 mM were beneficial to growth and proliferation, while higher levels did not affect these processes. The above range of iron concentrations also led to a more extensive iron accumulation, while further increase expressed no effect. So, Fe2+ concentration of 3.6 mM, enabling its high accumulation within the cells, while not negatively affecting biomass yield was selected for further studies. Iron uptake led to the modifications in transport of several other elements (Ca2+, Zn2+, K+ and Na+) and thus to the change in ion composition of the cells in comparison with the corresponding control.
Gligic
L
L
Galenika A.D. Pharmaceutical Works, Institute for Research and Development, Department of Biotechnology, Batajnicki drum bb, Belgrado, Serbia.
Vujovic
N
N
Stevovic
B
B
Manic
J
J
D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; D007501:Iron