Southern blot analysis of individual Drosophila flies.
Detection of novel insertions and the ability to explore heterochromatin are two key goals in the study of mobile elements in Drosophila. The Southern blot analysis of individual flies can prove useful in both types of investigations, alone or in combination with genetic and cytological approaches. This chapter describes the protocol for carrying out such an analysis and provides some clues for the interpretation of the results.
Junakovic
Nikolaj
N
Istituto di Biologia e Patologia Molecolari CNR, Università La Sapienza, Roma, Italy.
D000818:Animals; D015139:Blotting, Southern / Q000379:methods*; D002875:Chromosomes; D015342:DNA Probes; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D006570:Heterochromatin; D007814:Larva / Q000235:genetics; D008297:Male
D015342:DNA Probes; D004251:DNA Transposable Elements; D006570:Heterochromatin