Contractile cardiac grafts using a novel nanofibrous mesh.
Cardiomyoctes are terminally differentiated cells and therefore unable to regenerate after infarction. The use of autologous bioengineered cardiac grafts has been suggested to replace infarcted myocardium and enhance cardiac function. Here we report the development of an in vitro system for engineered myocardium. Cardiac nanofibrous meshes (CNM) were developed by culturing cardiomyocytes from neonatal Lewis rats on electrospun, nanofibrous polycaprolactone (PCL) meshes. The mesh had an ECM-like topography and was suspended across a wire ring that acted as a passive load to contracting cardiomyocytes. The cardiomyocytes started beating after 3 days and were cultured in vitro for 14 days. The cardiomyocytes attached well on the PCL meshes and expressed cardiac-specific proteins such as alpha-myosin heavy chain, connexin43 and cardiac troponin I. The results demonstrate the formation of contractile cardiac grafts in vitro. Using this technique, cardiac grafts can be matured in vitro to obtain sufficient function prior to implantation. It is conjectured that cardiac grafts with clinically relevant dimensions can be obtained by stacking CNMs and inducing vascularization with angiogenic factors.
Shin
M
M
Department of Surgery, Massachusetts General Hospital and Harvard Medical School, Wellman 627, 55 Fruit Street, Boston, MA 02114, USA. mshin@partners.org
Ishii
O
O
Sueda
T
T
Vacanti
J P
JP
D000818:Animals; D000831:Animals, Newborn; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D004563:Electrochemistry / Q000379:methods; D008422:Materials Testing; D008567:Membranes, Artificial; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000502:physiology*; D036103:Nanotechnology / Q000379:methods; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure*; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D011917:Rats, Inbred Lew; D013784:Textiles; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011091:Polyesters; C016240:polycaprolactone