Role of maternal adrenal glands on the developing serotoninergic and aminoacidergic systems of the postnatal rat brain.
Serotonin, gamma-aminobutyric acid and glutamate, which are regulated by glucocorticoids in the central nervous system, are involved in neuroendocrine functions and the development of the brain. The present study investigates the effect of maternal adrenalectomy on the developing serotoninergic, GABAergic and glutamatergic systems. Neurotransmitter levels were measured in four brain areas of both male and female offspring on postnatal days 1, 8, 12 and 22. At postnatal day 1 and 8, the pups of adrenalectomized dams showed higher concentrations of serotonin than controls in all the brain areas studied. Serotonin levels decreased significantly in males at postnatal day 22 in the hippocampus and cortex. During the first 2 weeks of postnatal life, the lack of maternal corticosterone produced an increase in glutamate and a reduction in gamma-aminobutyric acid concentrations, mainly in males. Further, on postnatal day 1, increased serotonin and glutamate levels and lower levels of gamma-aminobutyric were observed in the hypothalamus of male pups born to adrenalectomized dams. The absence of maternal corticosterone affects the pattern of development of the serotoninergic system, especially in the hippocampus and cortex, and particularly in males. A delay in the maturation of the aminoacidergic systems, mainly of the GABAergic system and in males, was also seen. A sexually dimorphic response to the removal of maternal glucocorticoids was seen in terms of neurotransmitter levels, mainly in the hippocampus and hypothalamus.
Leret
M L
ML
Department of Animal Biology-II (Animal Physiology), Faculty of Biological Sciences, José Antonio Nováis, 2, Complutense University of Madrid, 28040 Madrid, Spain. mlleret@bio.ucm.es
Peinado
V
V
Suárez
L M
LM
Tecedor
L
L
Gamallo
A
A
González
J C
JC
D000311:Adrenal Glands / Q000378:metabolism / Q000502:physiology*; D000315:Adrenalectomy / Q000009:adverse effects*; D000818:Animals; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D015536:Down-Regulation / Q000502:physiology; D005260:Female; D005938:Glucocorticoids / Q000172:deficiency* / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism / Q000503:physiopathology; D007031:Hypothalamus / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D009035:Mothers; D009434:Neural Pathways / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism; D012727:Sex Characteristics; D015854:Up-Regulation / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D005938:Glucocorticoids; D018377:Neurotransmitter Agents; D012701:Serotonin; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid