Contribution of winery-resident Saccharomyces cerevisiae strains to spontaneous grape must fermentation.
The origin of the Saccharomyces cerevisiae strains that are responsible for spontaneous grape must fermentation was investigated in a long-established industrial winery by means of two different approaches. First, seven selected components of the analytical profiles of the wines produced by 58 strains of S. cerevisiae isolated from different sites and phases of the production cycle of a Grechetto wine were subjected to Principal Components Analysis. Secondly, the same S. cerevisiae isolates underwent PCR fingerprinting by means of delta primers. The results obtained by both methods demonstrate unequivocally that under real vinification conditions, the S. cerevisiae strains colonising the winery surfaces are the ones that carry out the natural must fermentation.
Ciani
Maurizio
M
Dipartimento di Scienze degli Alimenti, Università Politecnica delle Marche, 60121 Ancona, Italy. mciani@univpm.it
Mannazzu
Ilaria
I
Marinangeli
Paola
P
Clementi
Francesca
F
Martini
Alessandro
A
D001483:Base Sequence; D016172:DNA Fingerprinting; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics; D005285:Fermentation; D005511:Food Handling; D007558:Italy; D016133:Polymerase Chain Reaction; D012405:Round Window, Ear; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D027843:Vitis / Q000382:microbiology*; D014920:Wine / Q000382:microbiology*
D017931:DNA Primers; D004271:DNA, Fungal