Determination of paeoniflorin in rat hippocampus by high-performance liquid chromatography after intravenous administration of Paeoniae Radix extract.
A rapid and simple high-performance liquid chromatographic (HPLC) assay for the determination of paeoniflorin in rat hippocampus was developed in this study. The chromatographic analysis was carried out using reversed-phase isocratic elution with a Zorbax SB-C(18) column, a mobile phase of methanol-water (32:68, v/v), and detection by ultraviolet (UV) absorption at 233 nm. The lower limits of quantitation (LLQ) were 1 microg/ml for paeoniflorin. The calibration curve for paeoniflorin was linear (r = 0.9999) over the concentration range of 1-50 microg/ml. The coefficients of variation of intra- and inter-day assays were 7.00, 0.58, 1.46% and 5.48, 1.79, 1.70% at concentrations of 1, 10, 50 microg/ml, respectively. The recoveries of paeoniflorin from rat hippocampus were 98.28 +/- 2.14, 98.96 +/- 1.48, and 95.34 +/- 0.92 at concentrations of 1, 10 and 50 microg/ml, respectively. Stability studies showed that paeoniflorin was stable at temperatures of 2-8 degrees C in methanol for at least 20 days. The method was applied to determine the time course of paeoniflorin in rat hippocampus, following the administration of a 60 mg/kg i.v. dose of paeoniflorin in Paeoniae Radix extract to a male Wistar rat.
He
Xihui
X
Chinese Academy of Medical Sciences and Peking Union Medical College, Institute of Medicinal Plant, Beijing 100094, China.
Xing
Dongming
D
Ding
Yi
Y
Li
Yaping
Y
Xiang
Lan
L
Wang
Wei
W
Du
Lijun
L
D000818:Animals; D001565:Benzoates / Q000032:analysis*; D001952:Bridged-Ring Compounds / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005960:Glucosides / Q000032:analysis*; D006624:Hippocampus / Q000737:chemistry*; D008297:Male; D039821:Monoterpenes; D010936:Plant Extracts / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D006027:Glycosides
D001565:Benzoates; D001952:Bridged-Ring Compounds; D005960:Glucosides; D039821:Monoterpenes; D010936:Plant Extracts; C015423:peoniflorin; D006027:Glycosides