title: Differential polypeptide display: the search for the elusive target.
abstract: Proteomics, as a tool to identify proteins in biological samples, is gaining rapidly importance in the postgenomic era. Here we discuss the current and potential role of different techniques in the field of proteomics such as two-dimensional gel electrophoresis off-line coupled to MALDI-MS (2D-PAGE-MALDI-MS), high performance liquid chromatography mass spectrometry (HPLC-MS), surface enhanced laser desorption/ionization mass spectrometry (SELDI-MS) and a newly developed technique, capillary electrophoresis mass spectrometry (CE-MS). The developments of the last years are presented discussed.
authors.affiliation: Mosaiques Diagnostics and Therapeutics AG, Hannover, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Wittke
authors.forename: Thorsten
authors.initials: T
authors.lastname: Kaiser
authors.forename: Harald
authors.initials: H
authors.lastname: Mischak
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D010455:Peptides