[Expression of heat shock proteins HSP72 and HSP73 in Colombian patients with Hodgkin lymphoma positive and negative for Epstein Barr virus].
BACKGROUND
The expression of heat shock proteins (HSP70) in tumor cells or virus infected cells is important for the induction of specific cellular immune response. They are implicated in transport of immunodominants peptides in the endoplasmic reticulum, activation of antigen presenting cells and cross priming of CD8 T cells.


OBJECTIVE
To analyze the expression of HSP70 protein in its constitutive (HSP73) and inducible forms (HSP72) in Hodgkin's lymphoma (HL), infected or not by Epstein Barr virus (EBV) and to assess its relationship with pathological subtype, clinical stages and treatment response.


METHODS
The analysis of HSP73 and HSP72 was done by immunoperoxidase on routinely processed paraffin sections with prior antigen retrieval.


RESULTS
Sixty seven cases were studied. The expression of HSP73 and HSP72 was detected in 19.4 and 17.9% of samples respectively. The infiltrating lymphocytes expressed HSP72 in 58% of cases. The pathological subtypes with the higher expression in lymphocytes were mixed cellularity and nodular sclerosis. No differences in HSP70 expression were observed, according to clinical stage, treatment response or the presence of EBV.


CONCLUSIONS
The expression of HSP72 on lymphocytes suggests that this protein plays an important role in the induction and amplification of antitumor immune response.
Quijano
Sandra M
SM
Laboratorio de Inmunología, Instituto Nacional de Cancerología, Bogotá, Colombia.
Saavedra
Carlos
C
Bravo
María Mercedes
MM
Fiorentino
Susana
S
Orozco
Oscar
O
D000293:Adolescent; D000328:Adult; D002352:Carrier Proteins / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D003105:Colombia; D005260:Female; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006689:Hodgkin Disease / Q000378:metabolism* / Q000821:virology*; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*
D002352:Carrier Proteins; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D050884:HSP72 Heat-Shock Proteins; C494371:HSPA8 protein, human; D006360:Heat-Shock Proteins; D009363:Neoplasm Proteins