title: Characteristic biological activities of lipopolysaccharides from Sinorhizobium and Mesorhizobium.
abstract: The biological actions of lipopolysaccharides (LPSs) from Sinorhizobium meliloti, Mesorhizobium loti and Escherichia coli were compared. In biological activities including lethality, production of tumor necrosis factor (TNF)-alpha and nitric oxide (NO), adjuvant action and Limulus activity, LPS from S. meliloti exhibited stronger actions than LPS from M. loti, but had a weaker action than LPS from E. coli. On the other hand, M. loti LPS showed a higher activity to activate human complement than S. meliloti LPS. Further, there was a significant difference in polymyxin B binding between S. meliloti LPS and M. loti LPS, suggesting a difference in the lipid A structure. LPSs from S. meliloti and M. loti seem to exhibit characteristic biological actions that may be dependent on the difference in the lipid A structure.
authors.affiliation: Department of Natural Science Informatics, School of Informatics and Sciences & Department of Microbiology and Immunology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan.
authors.forename: Yasuyo
authors.initials: Y
authors.lastname: Tsukushi
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kido
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Saeki
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Naoki
authors.initials: N
authors.lastname: Koide
authors.forename: Isamu
authors.initials: I
authors.lastname: Mori
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Yoshida
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokochi
mesh_terms: D000818:Animals; D001681:Biological Assay; D004926:Escherichia coli / Q000472:pathogenicity; D006737:Horseshoe Crabs; D008070:Lipopolysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D009569:Nitric Oxide / Q000032:analysis; D020560:Proteobacteria / Q000472:pathogenicity*; D020579:Sinorhizobium / Q000472:pathogenicity*; D013329:Structure-Activity Relationship; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide