Role of ELISA in H. pylori detection and its correlation with urease test.
Helicobacter pylori is one of the most common chronic bacterial infection in humans linked to acid peptic diseases, gastric carcinomas and lymphomas. The bacilli produces large amounts of urease and this property has formed the basis of detection of H. pylori by the Christensen's urease test. Where endoscopy is not clinically indicated, serology may be used to establish the diagnosis. This study was undertaken to diagnose H. pylori with the help of Christensen's urease test on endoscopic biopsy specimens & correlated with the detection in Sera, of IgG antibodies against H. pylori, by ELISA technique. The study was conducted on 100 patients suffering from acid peptic disorders out of which 40 (40%) tested positive for H. pylori both by urease and serology. Christensen's urease and ELISA were found to have sensitivities of 85.7% & 90.9% and specificities of 96% and 87.5% respectively. Christensen's urease was taken as a standard method of diagnosis and its correlation with ELISA worked out to (+1) which meant there was a strong positive association between both the tests. Hence either test could be used for primary diagnosis of H. pylori instead of histopathological study and/or culture of H. pylori.
Gupta
Piyalee
P
Department of Microbiology, Rajendra Medical College & Hospital, Ranchi.
Pandey
L B
LB
Behra
R N
RN
Rai
M K
MK
Chandra
R
R
D000907:Antibodies, Bacterial / Q000097:blood; D004415:Dyspepsia / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D004941:Esophagitis / Q000382:microbiology; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D016480:Helicobacter pylori / Q000201:enzymology / Q000276:immunology; D006801:Humans; D008297:Male; D010437:Peptic Ulcer / Q000382:microbiology; D014510:Urease / Q000032:analysis
D000907:Antibodies, Bacterial; D014510:Urease