On the role of Trk1 and Trk2 in Schizosaccharomyces pombe under different ion stress conditions.
Trk1 and Trk2 are the major K(+) transport systems in Schizosaccharomyces pombe. Both transporters individually seem to be able to cope with K(+) requirements of the cells under normal conditions, since only the double mutant shows defective K(+) transport and defective growth at limiting K(+) concentrations. We have studied in detail the role of SpTrk1 and SpTrk2 under different ion stress conditions. Results show that the strain with only Trk1 (trk1(+)) is less sensitive to Li(+) and to hygromycin B, it grows better at low K(+) and it survives longer in a medium without K(+) than the strain expressing only Trk2 (trk2(+)). We conclude that Trk1 contributes more efficiently than Trk2 to the performance of the fission yeast under ion stress conditions. In the wild type both trk1(+) and trk2(+) genes are expressed and probably collaborate for the performance of the cells.
Calero
Fernando
F
Departamento de Microbiologiá, Universidad de Córdoba, Campus Universitario de Rabanales Edif. Severo Ochoa, 14071 Córdoba, Spain.
Montiel
Vera
V
Caracuel
Zaira
Z
Cabello-Hurtado
Francisco
F
Ramos
José
J
D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D018869:Heat-Shock Response; D006921:Hygromycin B / Q000494:pharmacology; D008094:Lithium / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D012413:Rubidium / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D027682:Cation Transport Proteins; D003470:Culture Media; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; C085258:TRK2 protein, S cerevisiae; C068664:TRK1 protein, S cerevisiae; D006921:Hygromycin B; D008094:Lithium; D012413:Rubidium; D011188:Potassium