[Design of chimeric proteins based on a pentameric superhelical fragment of COMP. II. Properties of a hybrid, containing an VNTR (MUC1) antigen of human tumors].
An oligomeric chimeric protein DB-2 was constructed, to design drugs with antitumor activity and to develop highly sensitive immunospecific tests for the diagnostics of a wide variety of malignant epithelial cells in humans. DB-2 contains an immunodominant site of tetanus toxin and a fragment of the locus of the human tumor-associated antigen MUC1 with a variable number of tandem repeats. A pentameric superhelical fragment of the human cartilage oligomeric matrix protein was used as an oligomerization matrix. The expression of the protein in Escherichia coli cells was studied, a method for its purification was developed, and its main biochemical properties were determined. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 1; see also http://www.maik.ru.
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Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP Moscow, 117997 Russia. lap08@mail.ru
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Korobko
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D001483:Base Sequence; D001704:Biopolymers; D064236:Cartilage Oligomeric Matrix Protein; D017931:DNA Primers; D016326:Extracellular Matrix Proteins / Q000737:chemistry* / Q000235:genetics; D006023:Glycoproteins / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D064235:Matrilin Proteins; D018598:Minisatellite Repeats; D018396:Mucin-1 / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D009369:Neoplasms / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics
D001704:Biopolymers; D064236:Cartilage Oligomeric Matrix Protein; D017931:DNA Primers; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D064235:Matrilin Proteins; D018396:Mucin-1; D011993:Recombinant Fusion Proteins; C577553:TSP5 protein, human