Malassezia nana sp. nov., a novel lipid-dependent yeast species isolated from animals.
Five isolates of a novel species of the yeast genus Malassezia were isolated from animals in Japan and Brazil. Phylogenetic trees based on the D1/D2 domains of the large-subunit (26S) rDNA sequences and nucleotide sequences of the internal transcribed spacer 1 region showed that the isolates were conspecific and belonged to the genus Malassezia. They were related closely to Malassezia dermatis and Malassezia sympodialis, but were clearly distinct from these two species and the other six species of Malassezia that have been reported, indicating that they should be classified as a novel species, Malassezia nana sp. nov. Morphologically and physiologically, M. nana resembles M. dermatis and M. sympodialis, but can be distinguished from these species by its inability to use Cremophor EL (Sigma) as the sole lipid source and to hydrolyse aesculin. The type strain of M. nana is NUSV 1003(T) (=CBS 9557(T)=JCM 12085(T)).
Hirai
Asuka
A
Department of Pathobiology, Nihon University School of Veterinary Medicine, Kameino, Fujisawa, Kanagawa 252-8510, Japan.
Kano
Rui
R
Department of Pathobiology, Nihon University School of Veterinary Medicine, Kameino, Fujisawa, Kanagawa 252-8510, Japan.
Makimura
Koichi
K
Teikyo University Institute of Medical Mycology, Otsuka, Hachioji, Tokyo 192-0395, Japan.
Duarte
Eduardo Robson
ER
Department of Microbiology, Biological Science Institute, Federal University of Minas Gerais, Avenida Antonio Carlos, Belo Horizonte, Minas Gerais, 31270-901, Brazil.
Hamdan
Júnia Soares
JS
Department of Microbiology, Biological Science Institute, Federal University of Minas Gerais, Avenida Antonio Carlos, Belo Horizonte, Minas Gerais, 31270-901, Brazil.
Lachance
Marc-André
MA
Department of Biology, University of Western Ontario, London, Ontario, Canada N6A 5B7.
Yamaguchi
Hideyo
H
Teikyo University Institute of Medical Mycology, Otsuka, Hachioji, Tokyo 192-0395, Japan.
Hasegawa
Atsuhiko
A
Department of Pathobiology, Nihon University School of Veterinary Medicine, Kameino, Fujisawa, Kanagawa 252-8510, Japan.
D000818:Animals; D001483:Base Sequence; D001938:Brazil; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D007564:Japan; D050356:Lipid Metabolism; D008290:Malassezia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D017931:DNA Primers; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012331:RNA, Fungal; D012336:RNA, Ribosomal, 16S