Growth performance and histological intestinal alterations in piglets fed dietary raw and heated pigeon pea seed meal.
Histological intestinal villus alterations were studied in piglets fed raw (PM) or heated (HPM) pigeon pea seed meal. The trypsin inhibition rate was 99.15% in PM and 54.31% HPM. The PM and HPM were added into the basal diet (crude protein; 176.3 g/kg, gross energy; 4.15 kcal/g, control) at 20% and 40% levels, respectively. The diets were formulated in order to adjust protein to 180 g/kg and gross energy to about 4.20 kcal/g. The feed intake was not different among groups. The daily body weight gain and feed efficiency tended to decrease with the increasing PM level, and they decreased significantly in the 40% PM group compared with the control group (P < 0.05). However, HPM groups showed a growth performance similar to the control. The villus height, cell area and cell mitosis tended to decrease with the increasing PM level, and they decreased significantly in the 40% PM group compared with the control group (P < 0.05). In HPM group, these villus height, cell area and cell mitosis were significantly higher than those of the 40% PM group (P < 0.05), and did not show a significant difference compared with the control. Compared with the duodenal villus surface of the control group, the PM groups had a smooth surface due to flat cells and the HPM group showed a rough surface due to protuberated cells. The current histological alterations of intestinal villi demonstrate that the villi might be atrophied in the piglets fed raw PM due to anti-nutritional factors, resulting in the decreased growth performance, and that heating PM might abolish such a harmful effect of the anti-nutritional factors on the villus function, resulting in a similar growth performance to the control. Raw PM could be incorporated under a level of 40%, but heated PM increases the incorporation rate up to the 40% level.
Mekbungwan
A
A
Department of Animal Technology, Faculty of Agricultural Production, Maejo University, Chiang Mai, Thailand.
Yamauchi
K
K
D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001835:Body Weight; D002455:Cell Division; D004043:Dietary Fiber / Q000378:metabolism; D004044:Dietary Proteins / Q000378:metabolism; D004063:Digestion; D004386:Duodenum / Q000378:metabolism; D006361:Heating; D007082:Ileum / Q000378:metabolism; D007408:Intestinal Absorption / Q000502:physiology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000502:physiology; D007583:Jejunum / Q000378:metabolism; D008297:Male; D008855:Microscopy, Electron, Scanning; D012639:Seeds / Q000378:metabolism; D013552:Swine; D014357:Trypsin / Q000737:chemistry; D015430:Weight Gain
D004043:Dietary Fiber; D004044:Dietary Proteins; D014357:Trypsin