title: Immunomodulating effect of antimicrobial agents on cytokine production by human polymorphonuclear neutrophils.
abstract: It has been previously demonstrated that some antimicrobial agents enhance activities of human polymorphonuclear neutrophils (PMNs). The effect on the release of cytokines in an inflammatory context from PMNs by these antibiotics was evaluated. We studied the effect of the release of some cytokines by human PMNs RT-PCR analysis on a clinical strain of Klebsiella pneumoniae by comparing the effect with that observed in the presence of co-amoxiclav, sanfetrinem, clarithromycin, prulifloxacin and tobramycin. All the drugs tested were capable of modulating PMN synthesis in vitro of pro-inflammatory cytokines IL-8, IL-1beta, TNF-alpha and IL-6, but not that of anti-inflammatory cytokine IL-10. The degree of their stimulatory or inhibitory potency varied with the cytokine examined.
authors.affiliation: Department of Biomedical Sciences and Human Oncology, University of Turin, Turin, Italy.
authors.forename: Gigliola
authors.initials: G
authors.lastname: Reato
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Cuffini
authors.forename: Vivian
authors.initials: V
authors.lastname: Tullio
authors.forename: Narcisa
authors.initials: N
authors.lastname: Mandras
authors.forename: Janira
authors.initials: J
authors.lastname: Roana
authors.forename: Giuliana
authors.initials: G
authors.lastname: Banche
authors.forename: Robin
authors.initials: R
authors.lastname: Foa
authors.forename: Nicola A
authors.initials: NA
authors.lastname: Carlone
mesh_terms: D000900:Anti-Bacterial Agents / Q000276:immunology* / Q000494:pharmacology; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007249:Inflammation / Q000276:immunology; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D018375:Neutrophil Activation / Q000187:drug effects; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism*; D016133:Polymerase Chain Reaction
chemical_list: D000900:Anti-Bacterial Agents; D016207:Cytokines; D008070:Lipopolysaccharides