[Expression of fascin 1 gene in the process of the immortalized esophageal carcinoma carcinogenesis].
OBJECTIVE
Fascin 1 is the 55kDa F-actin- binding cytoskeleton protein. Fascin 1 gene was cloned from a human teratocarcinoma. Up to now, the carcinogenesis mechanism of esophageal squamous cell carcinoma is unclear. The study was designed to identify the differentially expressed proteins and mRNAs between the human immortalized esophageal epithelial cell line (SHEE) transfected by human papillomavirus type 18 E6E7 and the malignant transformation cell line (SHEEmt), which is derivated from SHEE, and to further understand the carcinogenesis mechanisms of esophageal squamous cell carcinoma.


METHODS
Cellular proteins were separated by two-dimensional electrophoresis and differentially expressed proteins were identified by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS). The mRNA of fascin 1 gene was assayed by reverse transcription polymerase chain reaction (RT-PCR) and its product was analyzed by sequencing assay.


RESULTS
The results manifested 9 genes expressed differently in the progress of malignant transformation of SHEE to SHEEmt, fascin 1 protein increased about 3.64 times and its mRNA increased about 16.17 times.


CONCLUSIONS
The upregulated expression of fascin 1 gene may be correlated with the malignant transformation of SHEE to SHEEmt.
Rong
Ju
J
Department of Biochemistry and Molecular Biology, Shantou University Medical College, Shantou, Guangdong, 515041 PR China.
Xu
Li-Yan
LY
Cai
Wei-Jia
WJ
Xiong
Xing-Dong
XD
Li
Jin-Tao
JT
Fang
Wang-Kai
WK
Shen
Zhong-Ying
ZY
Li
En-Min
EM
D002352:Carrier Proteins / Q000235:genetics*; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000209:etiology / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008840:Microfilament Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis
D002352:Carrier Proteins; D008840:Microfilament Proteins; D012333:RNA, Messenger; C020324:fascin