Regulation of human peripheral blood lymphocytes IL-10 BY SMS 201-995.
The somatostatin analog SMS 201-995 inhibits human peripheral blood lymphocytes (PBL) proliferation and here we demonstrate that it induces a significant increase in T cells IL-10 release as is evidenced in double fluorescence experiments. Seizing IL-10 by monoclonal antibody, SMS does not affect lymphocyte proliferation, suggesting that this cytokine is involved in the antiproliferative effect of these analog. We previously demonstrated that SMS inhibits T cells acting on the CD28 rather than the CD3-mediated signal in exactly the same way as does IL-10. Thus SMS inhibits human PBL activation by inducing IL-10 release and the consequent inhibition of the CD28 co-stimulatory pathway providing new perspectives on developing immunosuppressive strategies.
Casnici
Claudia
C
Department of Pharmacology, School of Medicine, University of Milan, Via Vanvitelli 32, Milan 20129, Italy.
Lattuada
Donatella
D
Franco
Paola
P
Cattaneo
Luana
L
Marelli
Ornella
O
D000328:Adult; D000906:Antibodies / Q000494:pharmacology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000187:drug effects / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004347:Drug Interactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology / Q000378:metabolism*; D007519:Isoantigens / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008875:Middle Aged; D015282:Octreotide / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D013004:Somatostatin / Q000031:analogs & derivatives*; D013997:Time Factors
D000906:Antibodies; D000945:Antigens, Differentiation, T-Lymphocyte; D007519:Isoantigens; D010835:Phytohemagglutinins; D016753:Interleukin-10; D013004:Somatostatin; D015282:Octreotide