Streptococcus mutans fructosyltransferase interactions with glucans.
Streptococcus mutans utilizes sucrose to synthesize glucans by glucosyltransferase and fructans by fructosyltransferase (FTF). Antibodies raised against a recombinant FTF were used to study S. mutans FTF secretion. Low amounts of cell-free FTF were found in culture of S. mutans grown with sucrose, while an increase in bacteria displaying cell surface FTF was detected. FTF added to S. mutans cultures was adsorbed to bacteria grown with sucrose but not to bacteria grown with glucose or fructose or to a gtf inactivated mutant grown with sucrose. Recombinant FTF was found to have high affinity for glucans suggesting that fructans and glucans are an integral part of the polysaccharide matrix of oral biofilms.
Rozen
Ramona
R
Institute of Dental Sciences, Faculty of Dentistry, Hebrew University-Hadassah, P.O. Box 12272, Jerusalem 91120, Israel.
Steinberg
Doron
D
Bachrach
Gilad
G
D018441:Biofilms; D003470:Culture Media / Q000737:chemistry; D005630:Fructans / Q000378:metabolism; D005632:Fructose / Q000378:metabolism; D017353:Gene Deletion; D005936:Glucans / Q000096:biosynthesis / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D005964:Glucosyltransferases / Q000235:genetics; D006602:Hexosyltransferases / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D011135:Polysaccharides, Bacterial; D011485:Protein Binding; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013295:Streptococcus mutans / Q000201:enzymology* / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism; D001426:Bacterial Proteins
D003470:Culture Media; D005630:Fructans; D005632:Fructose; D005936:Glucans; D005947:Glucose; D005964:Glucosyltransferases; D006602:Hexosyltransferases; D011135:Polysaccharides, Bacterial; D011994:Recombinant Proteins; D013395:Sucrose; C021451:inulosucrase; D001426:Bacterial Proteins