[Determination of puerarin, daidzin and daidzein in root of Pueraria lobata of different origin by HPLC].
OBJECTIVE
To develop an HPLC method to determine Puerarin, Daidzin and Daidzein in Pueraria lobata.


METHODS
The separation was performed in a SYMMETRY C18 column with a mobile phase of methanol-1% HAC solution (gradient elution), the The detection wavelength being 250 nm.


RESULTS
The average recovery was respectively 101.7%, 100.7% and 101.7% (n = 3), RSD 0.43%, 0.82% and 1.50% (n = 3) for Puerarin, Daidzin and Daidzein.


CONCLUSIONS
This method is suitable for the determination of Puerarin, Daidzin and Daidzein in Pueraria lobata and its preparation.
Jin
Wen-shan
WS
Beijing Yuke Botanical Development CO., LTD., Beijing 100062, China. jinwenshan@yahoo.com.cn
Tan
Yu-yuan
YY
Chen
You-gen
YG
Wang
Yan
Y
D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D017753:Ecosystem; D007529:Isoflavones / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D029908:Pueraria / Q000737:chemistry*; D011786:Quality Control
D007529:Isoflavones; C013908:daidzin; C004742:daidzein; C033607:puerarin