Green tea polyphenols inhibit advanced glycation end product-induced rat vascular smooth muscle cell proliferation.
OBJECTIVE
To determine the effects of green tea polyphenols(GTP) on advanced glycation end products (AGEs)-induced proliferation and expression of p44/42 mitogen-activated protein kinase (MAPK) of rat vascular smooth muscle cells (VSMCs)


METHODS
Rat aortic VSMCs isolated and cultured in vitro were stimulated with AGEs in the presence or absence of GTP at different concentrations, followed by quantitative analysis of the cell proliferation with colorimetric assay. The p44/42 MAPK activity was evaluated by immunoblotting technique using anti-p44/42 phospho-MAPK antibody.


RESULTS
Compared with the control cells(without GTP treatment), GTP dose-dependently inhibited AGE-stimulated VSMC proliferation (P<0.05), and the p44/42 MAPK activity was significantly enhanced. The effects of AGEs were antagonized by GTP (372+/-41 vs 761+/-56, P<0.05).


CONCLUSIONS
GTP can inhibit the AGE-induced proliferation and p44/42 MAPK expression of rat VSMCs.
Ouyang
Ping
P
Department of Cardiology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. ypou@fimmu.com
Peng
Wen-lie
WL
Xu
Ding-li
DL
Lai
Wen-yan
WY
Xu
An-long
AL
D000818:Animals; D001161:Arteriosclerosis / Q000517:prevention & control*; D002478:Cells, Cultured; D005419:Flavonoids / Q000494:pharmacology*; D017127:Glycation End Products, Advanced / Q000633:toxicity*; D006160:Guanosine Triphosphate / Q000494:pharmacology; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology*; D059808:Polyphenols; D051381:Rats; D017207:Rats, Sprague-Dawley; D013662:Tea
D005419:Flavonoids; D017127:Glycation End Products, Advanced; D010636:Phenols; D059808:Polyphenols; D013662:Tea; D006160:Guanosine Triphosphate; D020928:Mitogen-Activated Protein Kinases