title: Human papillomavirus (HPV) origin-binding protein associates with mitotic spindles to enable viral DNA partitioning.
abstract: Human papillomaviruses (HPVs) establish long-term infections in patients. The mechanism for extrachromosomal HPV DNA persistence in cycling cells is unknown. We show that HPV origin-containing plasmids partition as minichromosomes, attributable to an association of the viral origin recognition protein E2 with mitotic spindles. alpha-, beta-, and gamma-tubulins were pulled down with a tagged E2. The N-terminal transacting and C-terminal protein dimerization/DNA binding domains independently associated with the spindles. We suggest that this E2 property enables these viruses to establish persistence. Its implication for HPV oncogenesis is presented.
authors.affiliation: Department of Molecular Pathology, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, AL 35294-0005, USA.
authors.forename: Brian A
authors.initials: BA
authors.lastname: Van Tine
authors.forename: Luan D
authors.initials: LD
authors.lastname: Dao
authors.forename: Shwu-Yuan
authors.initials: SY
authors.lastname: Wu
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Sonbuchner
authors.forename: Biing Yuan
authors.initials: BY
authors.lastname: Lin
authors.forename: Nianxiang
authors.initials: N
authors.lastname: Zou
authors.forename: Cheng-Ming
authors.initials: CM
authors.lastname: Chiang
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Broker
authors.forename: Louise T
authors.initials: LT
authors.lastname: Chow
mesh_terms: D018385:Centrosome / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D022101:Microtubule-Organizing Center / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D027383:Papillomaviridae / Q000378:metabolism*; D010957:Plasmids; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C055789:origin-binding proteins, viral