title: The occurrence of NADPH-ferrihemoprotein reductase in Corbula caribea, from a natural oil seep at La Brea, Trinidad.
abstract: Corbula caribea is the most common non-polychaete macrofaunal organism identified at a large natural oil seep at La Brea in south Trinidad. It is hypothesized that these animals may possess (NADPH-ferrihemoprotein reductase) a component of the Mixed Function Oxygenase system (MFO), which may allow them to ameliorate the potentially deleterious effects resulting from exposure to the high levels of petroleum hydrocarbons within this environment. This study was designed to determine whether organisms from the seep site showed greater enzyme activity when compared to organisms from a non-seep reference site. NADPH-ferrihemoprotein reductase activity was determined by incubating 10 microm cryostat sections with nitro-blue tetrazolium. The reaction product was determined by visual assessment and quantified by measuring the relative mean stain intensity. The intense staining, indicative of enzyme activity was evident in the digestive epithelia of seep animals. Observations indicated that organisms from the seep showed more intense staining, indicating greater enzyme activity, when compared to animals from a non-seep reference site. The relative stain intensity of NADPH-ferrihemoprotein reductase determined for organisms from the seep was 61.30. This was significantly higher than the stain intensity determined for organisms from the non-seep reference site (7.11). This supported visual assessments, which suggested that the seep organisms showed higher enzyme activity than organisms from the non-seep site. The results suggest that NADPH-ferrihemoprotein reductase may be present in Corbula caribea from the seep site and not in those from the non-seep site. It is possible that this enzyme may contribute to these animals ability to tolerate chronic exposure to petroleum hydrocarbons and offer then a selective advantage for survival the seep environment.
authors.affiliation: Department of Life Sciences, The University of the West Indies, St. Augustine, Trinidad, Trinidad and Tobago. mohc0134@trini.com
authors.forename: Azad
authors.initials: A
authors.lastname: Mohammed
authors.forename: John
authors.initials: J
authors.lastname: Agard
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D049872:Bivalvia / Q000201:enzymology*; D006651:Histocytochemistry; D009251:NADPH-Ferrihemoprotein Reductase / Q000032:analysis*; D009580:Nitroblue Tetrazolium; D010578:Petroleum / Q000378:metabolism*
chemical_list: D010578:Petroleum; D009580:Nitroblue Tetrazolium; D009251:NADPH-Ferrihemoprotein Reductase