[The inhibitive effect of tea polyphenols on oxidized low density lipoprotein intake in cultured rat mesangial cells].
OBJECTIVE
To study the inhibitive effect of tea polyphenols (TP) on oxidized low density lipoprotein (OX-LDL) intake in cultured rat mesangial cells.


METHODS
Rat mesangial cells incubated in 80 mg.L-1 OX-LDL were treated with TP at different concentrations. Total cholesterol (TC) content of the mesangial cells and proportion of mesangial cells containing lipid droplets were measured, and ultrastructure observation was performed.


RESULTS
Foam cells were observed in mesangial cells stimulated by OX-LDL, and both the TC content and the proportion of lipid-droplet-containing cell were higher in these cells than in mesangial cells cultured in lipid free conditions. There were concentration-dependent decreases both in TC content and lipid-droplet-containing cell proportions when treated with TP from 40 ng.L-1 to 40 mg.L-1. The number of lipid droplets was also decreased in TP treated mesangial cells.


CONCLUSIONS
TP has an inhibitive effect on OX-LDL intake in cultured rat mesangial cells, which is in a concentration-dependent manner from 40 ng.L-1 to 40 mg.L-1.
Zhang
Yan
Y
Department of Nephrology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Gan
Hua
H
D000818:Animals; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D005487:Foam Cells / Q000378:metabolism; D005920:Glomerular Mesangium / Q000166:cytology / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008297:Male; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D059808:Polyphenols; D051381:Rats; D017208:Rats, Wistar; D013662:Tea / Q000737:chemistry
D005419:Flavonoids; D008077:Lipoproteins, LDL; D010636:Phenols; D059808:Polyphenols; D013662:Tea; C099252:oxidized low density lipoprotein; D002784:Cholesterol