title: Broad-spectrum immunity against superantigens is elicited in mice protected from lethal shock by a superantigen antagonist peptide.
abstract: Bypassing the restricted presentation of conventional antigens, superantigens trigger an excessive cellular immune response leading to toxic shock. Antagonist peptides that inhibit the induction of human Th1 cytokine gene expression by a variety of bacterial superantigens protect mice from lethal toxic shock. We show that the surviving mice rapidly develop a broad-spectrum, protective immunity against further lethal toxin challenges with the same superantigen and even with superantigen toxins that they have not encountered before. By blocking the induction of a cellular immune response leading to toxic shock, the antagonist peptide allows the superantigen to induce a vigorous humoral immune response directed against itself, resulting in anti-toxin IgM and IgG that are broadly protective. Adoptive transfer of these antibodies to naïve mice rendered them resistant to lethal superantigen challenge. The appearance of these antibodies does not require immunization with an adjuvant and is not elicited by the antagonist peptide. Our results show that superantigens are potent immunogens when given the opportunity to induce a B cell response, in conditions where a deleterious Th1 response is prevented by the superantigen antagonist peptide.
authors.affiliation: Department of Molecular Virology, Hadassah Medical School, The Hebrew University, 91120 Jerusalem, Israel.
authors.forename: Gila
authors.initials: G
authors.lastname: Arad
authors.forename: Dalia
authors.initials: D
authors.lastname: Hillman
authors.forename: Revital
authors.initials: R
authors.lastname: Levy
authors.forename: Raymond
authors.initials: R
authors.lastname: Kaempfer
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D004768:Enterotoxins / Q000276:immunology*; D005098:Exotoxins / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000494:pharmacology*; D012772:Shock, Septic / Q000276:immunology* / Q000517:prevention & control*; D018089:Superantigens / Q000276:immunology*; D015996:Survival Rate; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004768:Enterotoxins; D005098:Exotoxins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008565:Membrane Proteins; D010455:Peptides; C117980:SpeA protein, Streptococcus pyogenes; D018089:Superantigens; C031818:enterotoxin B, staphylococcal