Gas chromatographic method for analysis of conjugated linoleic acids isomers (c9t11, t10c12, and t9t11) in broth media as application in probiotic studies.
A gas chromatography (GC) procedure is assayed for analysis of conjugated linoleic acid (CLA) isomers cis-9, trans-11-octadecadienoic (c9t11); trans-10, 12 cis-octadecadienoic (t10c12); and trans-9, trans-11-octadecadienoic (t9t11) in culture broth by GC using NaOH-BF3 in methanol for methylating and a long capillary (100 m) high-polarity column. Repeatability of the method is assessed; the coefficient of variation for CLA isomers ranges from 4.62 for c9t11 to 8.19 for t9t11. Recovery ranges between 88.01 and 89.76, with a mean value of 89.06 for all CLA isomers studied. This method may be considered advantageous for analysis of CLA isomers in probiotics cultures samples.
Alonso
L
L
Department of Animal Science and Food and Agricultural Product Research and Technology Center, Oklahoma State University, Stillwater, OK 74078, USA. lalonso@ipla.csic.es
Cuesta
E P
EP
Gilliland
S E
SE
D002849:Chromatography, Gas / Q000379:methods*; D007536:Isomerism; D008041:Linoleic Acids / Q000032:analysis*; D019936:Probiotics / Q000737:chemistry*; D015203:Reproducibility of Results
D008041:Linoleic Acids