Assessing crystallization droplets using birefringence.
In this paper, the detection of crystalline elements in protein crystallization droplets containing precipitate is illustrated using the rotating-polarizer microscope technique. The sensitivity of this automated birefringence technique enables the detection of microcrystals in a precipitate that appears to be amorphous using traditional methods of inspection. The technique is illustrated with lysozyme and glucose isomerase. Glucose isomerase microcrystals were used successfully for seeding experiments and the conditions of both of the systems were refined to produce crystals suitable for X-ray analysis. The results are relevant to the field of high-throughput crystallography as an automated crystal-detection method as well as being a useful tool for detailed precipitate analysis.
Echalier
A
A
University of Warwick, Department of Biological Sciences, Gibbet Hill Road, Coventry CV4 7AL, England.
Glazer
R L
RL
Fülöp
V
V
Geday
M A
MA
D019747:Aldose-Ketose Isomerases / Q000737:chemistry; D001718:Birefringence; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray; D009113:Muramidase / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*
D011506:Proteins; D009113:Muramidase; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase