Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation.
The present work compares six biochemical methods for extraction of lipids from human serum. Although some organic solvents were good lipid extractors, they precipitated most of the total proteins and albumin. On the other hand, methodologies using Triton X-114 and silica were efficient for extraction of lipids, while sparing the protein fraction.
Ferraz
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TP
Centro de Pesquisa Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil.
Fiúza
M C
MC
Dos Santos
M L A
ML
Pontes De Carvalho
L
L
Soares
N M
NM
D019411:Clinical Laboratory Techniques; D006801:Humans; D008055:Lipids / Q000737:chemistry / Q000302:isolation & purification*; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000737:chemistry; D011309:Preservation, Biological; D011506:Proteins / Q000737:chemistry; D012680:Sensitivity and Specificity; D044967:Serum / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
D008055:Lipids; D011506:Proteins; D012997:Solvents; D011092:Polyethylene Glycols; D012822:Silicon Dioxide; D017830:Octoxynol; C010615:Nonidet P-40