title: Determination of laboratory reference ranges for lymphocyte immunophenotype subsets in healthy adult Japanese using flow cytometry.
abstract: This study represents a comprehensive evaluation of normative values for lymphocyte immunophenotype subsets using flow cytometry techniques in a Japanese population. Lymphocyte reference ranges were determined for percentage and absolute count of T, B, and NK cells in healthy adult Japanese using an extensive two-color immunophenotyping panel and consistently applied quality control methodology. Reference values were also determined for activation markers on CD3+ lymphocytes CD3+/CD25+, CD3+/CD38+ and CD3+/HLA-DR+. Differences in age and gender were observed for specific lymphocyte subsets. Comparison of the Japanese study with a Thai multi-center study that used similar methodology also demonstrated ethnic differences in lymphocyte reference ranges. The results in this study strongly suggest that reference values derived from studies in one population may not be applied to another population even when similar protocols for reagents, instruments and procedures are used although such studies do appear useful for epidemiological comparisons.
authors.affiliation: BD Biosciences Immunocytometry Systems (BD Biosciences), San Jose, California, USA.
authors.forename: H Kyle
authors.initials: HK
authors.lastname: Webster
authors.forename: Yohko
authors.initials: Y
authors.lastname: Kawai
authors.forename: Douslas
authors.initials: D
authors.lastname: Haney
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakahara
authors.forename: Kiyoaki
authors.initials: K
authors.lastname: Watanabe
authors.forename: Etsuko
authors.initials: E
authors.lastname: Ogawa
authors.forename: Lupe
authors.initials: L
authors.lastname: Miller
authors.forename: Leonard
authors.initials: L
authors.lastname: Buchner
authors.forename: Michael
authors.initials: M
authors.lastname: Lock
authors.forename: Petera
authors.initials: P
authors.lastname: Thompson
mesh_terms: D036541:ADP-ribosyl Cyclase / Q000097:blood; D051997:ADP-ribosyl Cyclase 1; D000328:Adult; D000367:Age Factors; D015703:Antigens, CD / Q000097:blood; D000944:Antigens, Differentiation, B-Lymphocyte / Q000097:blood; D000945:Antigens, Differentiation, T-Lymphocyte / Q000097:blood; D001402:B-Lymphocytes / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D005260:Female; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000097:blood; D006801:Humans; D016130:Immunophenotyping; D007564:Japan; D007694:Killer Cells, Natural / Q000378:metabolism; D008213:Lymphocyte Activation; D018655:Lymphocyte Count; D008297:Male; D008562:Membrane Glycoproteins; D008875:Middle Aged; D015375:Receptors, Interleukin-2 / Q000097:blood; D012016:Reference Values; D012737:Sex Factors; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D015415:Biomarkers; D006684:HLA-DR Antigens; D008562:Membrane Glycoproteins; D015375:Receptors, Interleukin-2; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1