title: D8/17 and CD19 expression on lymphocytes of patients with acute rheumatic fever and Tourette's disorder.
abstract: D8/17, an alloantigen found on B lymphocytes, has been reported to be elevated in patients susceptible to rheumatic fever and may be associated with autoimmune types of neuropsychiatric disorders. The pediatric-autoimmune-neuropsychiatric-disorders-associated-with-streptococci model is a putative model of pathogenesis for a group of children whose symptoms of obsessive-compulsive disorder and Tourette's disorder (TD) are abrupt and may be triggered by an infection with group A streptococci. As a test of this model, we have examined D8/17 levels on the B cells of patients with TD and acute rheumatic fever (ARF) along with those on the B cells of normal controls by flow cytometry. We have utilized several different preparations of D8/17 antibody along with a variety of secondary antibodies but have been unable to show an association with an elevated percentage of D8/17-positive, CD19-positive B cells in either ARF or TD. We did find, however, that the percentages of CD19-positive B cells in ARF and TD patients were significantly elevated compared to those in normal controls. Group A streptococcal pharyngitis patients also had an elevated percentage of CD19 B cells, however. These studies failed to confirm the utility of determining the percentage of B cells expressing the D8/17 alloantigen in ARF patients or our sample of TD patients. In contrast, the percentage of CD19-positive B cells was significantly elevated in ARF and TD patients, as well as group A streptococcal pharyngitis patients, suggesting a role for inflammation and/or autoimmunity in the pathogenesis of these disorders.
authors.affiliation: Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA.
authors.forename: Julie L
authors.initials: JL
authors.lastname: Weisz
authors.forename: William M
authors.initials: WM
authors.lastname: McMahon
authors.forename: Jill C
authors.initials: JC
authors.lastname: Moore
authors.forename: Nancy H
authors.initials: NH
authors.lastname: Augustine
authors.forename: John F
authors.initials: JF
authors.lastname: Bohnsack
authors.forename: James F
authors.initials: JF
authors.lastname: Bale
authors.forename: Michael B
authors.initials: MB
authors.lastname: Johnson
authors.forename: Jubel F
authors.initials: JF
authors.lastname: Morgan
authors.forename: Jenise
authors.initials: J
authors.lastname: Jensen
authors.forename: Lloyd Y
authors.initials: LY
authors.lastname: Tani
authors.forename: L George
authors.initials: LG
authors.lastname: Veasy
authors.forename: Harry R
authors.initials: HR
authors.lastname: Hill
mesh_terms: D000208:Acute Disease; D018941:Antigens, CD19 / Q000276:immunology / Q000378:metabolism*; D015551:Autoimmunity / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D007519:Isoantigens / Q000276:immunology / Q000378:metabolism*; D010612:Pharyngitis / Q000276:immunology; D012213:Rheumatic Fever / Q000276:immunology*; D013290:Streptococcal Infections / Q000276:immunology; D005879:Tourette Syndrome / Q000276:immunology*
chemical_list: D018941:Antigens, CD19; D007519:Isoantigens