Genetic evidence that Weissella kimchii Choi et al. 2002 is a later heterotypic synonym of Weissella cibaria Björkroth et al. 2002.
The taxonomic positions of Weissella kimchii and Weissella cibaria were examined using phenotypic tests, 16S rRNA gene sequencing and DNA-DNA hybridization. The two species displayed very similar sugar-fermentation patterns and produced the D-form of lactic acid. Phylogenetic analysis showed that W. cibaria is the closest neighbour of W. kimchii, sharing about 99.7 % similarity in the 16S rRNA gene sequence. DNA-DNA hybridization experiments further reinforced these results, demonstrating a 98.2 % reassociation level between W. cibaria LMG 17699(T) and W. kimchii DSM 14295(T). On the other hand, strain RO5, an isolate from paddy rice silage, exhibited 99.4 % 16S rRNA gene sequence similarity and a DNA reassociation level of more than 97 % with both type strains. Therefore, it is proposed that the species W. kimchii should be reclassified as W. cibaria.
Ennahar
Saïd
S
National Institute of Livestock and Grassland Science, Department of Animal Feeding and Management, Nishinasuno, Tochigi 329-2793, Japan.
Cai
Yimin
Y
National Institute of Livestock and Grassland Science, Department of Animal Feeding and Management, Nishinasuno, Tochigi 329-2793, Japan.
D001407:Bacillus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S