title: Crystallization and preliminary X-ray study of the common edible mushroom (Agaricus bisporus) lectin.
abstract: The lectin from the common edible mushroom Agaricus bisporus (ABL) belongs to the group of proteins that have the property of binding the Thomsen-Friedenreich antigen (T-antigen) selectively and with high affinity, but does not show any sequence similarity to the other proteins that share this property. The ABL sequence is instead similar to those of members of the saline-soluble fungal lectins, a protein family with pesticidal properties. The presence of different isoforms has been reported. It has been found that in order to be able to grow diffraction-quality crystals of the lectin, it is essential to separate the isoforms, which was performed by preparative isoelectric focusing. Using standard procedures, it was possible to crystallize the most basic of the forms by either vapour diffusion or equilibrium dialysis, but attempts to grow crystals of the other more acidic forms were unsuccessful. The ABL crystals belong to the orthorhombic space group C222(1), with unit-cell parameters a = 93.06, b = 98.16, c = 76.38 A, and diffract to a resolution of 2.2 A on a conventional source at room temperature. It is expected that the solution of this structure will yield further valuable information on the differences in the T-antigen-binding folds and will perhaps help to clarify the details of the ligand binding to the protein.
authors.affiliation: Laboratorio di Biocristallografia, Dipartimento Scientifico e Tecnologico, Università di Verona, Strada Le Grazie 15, 37134 Verona, Italy.
authors.forename: Maria E
authors.initials: ME
authors.lastname: Carrizo
authors.forename: Fernando J
authors.initials: FJ
authors.lastname: Irazoqui
authors.forename: Ricardo D
authors.initials: RD
authors.lastname: Lardone
authors.forename: Gustavo A
authors.initials: GA
authors.lastname: Nores
authors.forename: Juan A
authors.initials: JA
authors.lastname: Curtino
authors.forename: Stefano
authors.initials: S
authors.lastname: Capaldi
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Perduca
authors.forename: Hugo L
authors.initials: HL
authors.lastname: Monaco
mesh_terms: D000364:Agaricus / Q000737:chemistry*; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D037102:Lectins / Q000737:chemistry*; D020033:Protein Isoforms / Q000737:chemistry
chemical_list: C041433:Agaricus lectins; D037102:Lectins; D020033:Protein Isoforms