title: Malassezia nana sp. nov., a novel lipid-dependent yeast species isolated from animals.
abstract: Five isolates of a novel species of the yeast genus Malassezia were isolated from animals in Japan and Brazil. Phylogenetic trees based on the D1/D2 domains of the large-subunit (26S) rDNA sequences and nucleotide sequences of the internal transcribed spacer 1 region showed that the isolates were conspecific and belonged to the genus Malassezia. They were related closely to Malassezia dermatis and Malassezia sympodialis, but were clearly distinct from these two species and the other six species of Malassezia that have been reported, indicating that they should be classified as a novel species, Malassezia nana sp. nov. Morphologically and physiologically, M. nana resembles M. dermatis and M. sympodialis, but can be distinguished from these species by its inability to use Cremophor EL (Sigma) as the sole lipid source and to hydrolyse aesculin. The type strain of M. nana is NUSV 1003(T) (=CBS 9557(T)=JCM 12085(T)).
authors.affiliation: Department of Pathobiology, Nihon University School of Veterinary Medicine, Kameino, Fujisawa, Kanagawa 252-8510, Japan.
authors.forename: Asuka
authors.initials: A
authors.lastname: Hirai
authors.affiliation: Department of Pathobiology, Nihon University School of Veterinary Medicine, Kameino, Fujisawa, Kanagawa 252-8510, Japan.
authors.forename: Rui
authors.initials: R
authors.lastname: Kano
authors.affiliation: Teikyo University Institute of Medical Mycology, Otsuka, Hachioji, Tokyo 192-0395, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Makimura
authors.affiliation: Department of Microbiology, Biological Science Institute, Federal University of Minas Gerais, Avenida Antonio Carlos, Belo Horizonte, Minas Gerais, 31270-901, Brazil.
authors.forename: Eduardo Robson
authors.initials: ER
authors.lastname: Duarte
authors.affiliation: Department of Microbiology, Biological Science Institute, Federal University of Minas Gerais, Avenida Antonio Carlos, Belo Horizonte, Minas Gerais, 31270-901, Brazil.
authors.forename: Júnia Soares
authors.initials: JS
authors.lastname: Hamdan
authors.affiliation: Department of Biology, University of Western Ontario, London, Ontario, Canada N6A 5B7.
authors.forename: Marc-André
authors.initials: MA
authors.lastname: Lachance
authors.affiliation: Teikyo University Institute of Medical Mycology, Otsuka, Hachioji, Tokyo 192-0395, Japan.
authors.forename: Hideyo
authors.initials: H
authors.lastname: Yamaguchi
authors.affiliation: Department of Pathobiology, Nihon University School of Veterinary Medicine, Kameino, Fujisawa, Kanagawa 252-8510, Japan.
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Hasegawa
mesh_terms: D000818:Animals; D001483:Base Sequence; D001938:Brazil; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D007564:Japan; D050356:Lipid Metabolism; D008290:Malassezia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012331:RNA, Fungal; D012336:RNA, Ribosomal, 16S