title: Induction of hepatic hematopoiesis with tenascin-C expression during the second trimester of development.
abstract: OBJECTIVE
  To determine the role of tenascin-C in fetal hepatic hematopoietic proliferation and differentiation in different stages of development.
  
  
  METHODS
  We examined and compared the immunohistochemical expression of tenascin-C in the hepatic stromal portal fields in the first, second, and third trimester of gestation respectively, in relation to the appearance of CD34 progenitor hematopoietic, stromal progenitor and vascular endothelial positive cells.
  
  
  RESULTS
  Our results demonstrated a quantitative difference in the second trimester of gestation concerning the expression of tenascin-C in the connective tissue stroma of the hepatic portal fields over the equivalent expression of the protein in the first (P<0.0001, t-test) and third trimester (P<0.0001, t-test). Similar changes in the above period were found concerning the expression of CD34 over the first (P<0.0001, t-test) and third trimesters (P<0.0001, t-test), suggesting a direct involvement of tenascin-C in the sustaining of hematopoietic activity.
  
  
  CONCLUSIONS
  Our data provide evidence that an ECM glycoprotein component, tenascin-C, plays a relevant role in hematopoiesis through interaction between stromal cells and hematopoietic progenitor cells.
authors.affiliation: Department of Histology-Embryology, Democritus University of Thrace, Dragana, Alexandroupolis 68 100, Greece. npapad@med.duth.gr
authors.forename: N
authors.initials: N
authors.lastname: Papadopoulos
authors.forename: C
authors.initials: C
authors.lastname: Simopoulos
authors.forename: J
authors.initials: J
authors.lastname: Sigalas
authors.forename: A
authors.initials: A
authors.lastname: Kotini
authors.forename: A
authors.initials: A
authors.lastname: Cheva
authors.forename: D
authors.initials: D
authors.lastname: Tamiolakis
mesh_terms: D005260:Female; D005333:Fetus / Q000378:metabolism*; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000502:physiology; D006801:Humans; D011247:Pregnancy / Q000378:metabolism*; D011264:Pregnancy Trimesters / Q000378:metabolism; D017154:Stromal Cells / Q000502:physiology; D019063:Tenascin / Q000378:metabolism*
chemical_list: D019063:Tenascin