title: [Laboratory-performance study of the notified methods to detect genetically modified maize (CBH351) and potato (NewLeaf Plus and NewLeaf Y)].
abstract: To investigate the key factors affecting the reliability of the analytical results, a laboratory-performance study was attempted for the notified methods to detect genetically modified (GM) maize (CBH351) and GM potato (NewLeaf Plus and NewLeaf Y). The test samples were designed as three pairs of blind duplicates, which included 0%, 0.1% and 1.0% GM maize (CBH351) or GM potato (NewLeaf Plus or NewLeaf Y). Fourteen laboratories participated in the study. The test samples were sent to the participating laboratories along with the protocol. The data were collected from all laboratories and statistically analyzed. For the 0% sample of the CBH351 maize, one laboratory reported a false-positive result. It was considered that contamination could have occurred via the common use of equipment or tools for the test. For the 0.1% samples of the NewLeaf Plus potato or NewLeaf Y potato, on the other hand, three laboratories reported false-negative results. It was presumed that these results were due to changes of the conditions of the electrophoresis and agarose-gel staining. The other laboratories reported appropriate results. It was considered that the method employed in this study was suitable for the assessment of laboratory performance.
authors.affiliation: National Institute of Health Sciences: 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Watanabe
authors.forename: Kikuko
authors.initials: K
authors.lastname: Kasama
authors.forename: Chiseko
authors.initials: C
authors.lastname: Wakui
authors.forename: Masaaki
authors.initials: M
authors.lastname: Shibuya
authors.forename: Akihiko
authors.initials: A
authors.lastname: Matsuki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Akiyama
authors.forename: Tamio
authors.initials: T
authors.lastname: Maitani
mesh_terms: D018744:DNA, Plant / Q000032:analysis; D030841:Food, Genetically Modified; D007753:Laboratories / Q000592:standards; D016133:Polymerase Chain Reaction; D011786:Quality Control; D011198:Solanum tuberosum; D003313:Zea mays
chemical_list: D018744:DNA, Plant