title: Cross-reactivity and inhibition of haemolysis by polyclonal antibodies raised against St II, a cytolysin from the sea anemone Stichodactyla helianthus.
abstract: The immunogenicity of sticholysin II (St II), a pore-forming polypeptide from the sea anemone Stichodactyla helianthus, was studied in rabbits using two adjuvants, Freund's and aluminium hydroxide. High titres of antibodies were raised against St II with Freund's adjuvant (FA). The structural homology between sticholysins I and II was also revealed by cross-reactivity assays. Since the oil constituent of FA neutralized the St II haemolytic activity, immunizations with St II-Freund's emulsions were carried out with the inactivated cytolysin. Purified anti-St II IgG also neutralized the St II haemolytic activity.
authors.affiliation: Centro de Estudio de Proteínas, Facultad de Biología, Universidad de La Habana, Calle 25 No. 455, entre J e I. Vedado, Ciudad Habana 10400, Cuba. mcpico@fbio.uh.cu
authors.forename: María
authors.initials: M
authors.lastname: Cristina Pico
authors.forename: Ariel
authors.initials: A
authors.lastname: Basulto
authors.forename: Alberto
authors.initials: A
authors.lastname: del Monte
authors.forename: Abdel
authors.initials: A
authors.lastname: Hidalgo
authors.forename: María
authors.initials: M
authors.lastname: Eliana Lanio
authors.forename: Carlos
authors.initials: C
authors.lastname: Alvarez
authors.forename: Eric
authors.initials: E
authors.lastname: Felicó
authors.forename: Anselmo
authors.initials: A
authors.lastname: Otero
mesh_terms: D000536:Aluminum Hydroxide / Q000276:immunology / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002846:Chromatography, Affinity; D003064:Cnidarian Venoms / Q000276:immunology* / Q000378:metabolism; D003429:Cross Reactions / Q000276:immunology; D003603:Cytotoxins / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005620:Freund's Adjuvant / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects*; D008074:Lipoproteins / Q000097:blood / Q000378:metabolism; D011817:Rabbits; D012615:Sea Anemones; D012799:Sialyltransferases / Q000276:immunology* / Q000378:metabolism; D013053:Spectrophotometry
chemical_list: D000906:Antibodies; D003064:Cnidarian Venoms; D003603:Cytotoxins; D008074:Lipoproteins; D000536:Aluminum Hydroxide; D005620:Freund's Adjuvant; D012799:Sialyltransferases; C043662:alpha-N-acetylneuraminate alpha-2,8-sialyltransferase