title: Purification, cloning and biological characterization of a novel disintegrin from Trimeresurus jerdonii venom.
abstract: A novel disintegrin, jerdonin, was purified from the Trimeresurus jerdonii venom by means of gel filtration and reverse phase high pressure liquid chromatography. Its coding cDNA was also isolated from the venom gland. The jerdonin coding cDNA is part of a precursor composed of proprotein, metalloproteinase, and disintegrin domains. From the deduced amino acid sequence, jerdonin is composed of 71 amino acid residues including 12 cysteines and the tripeptide sequence Arg-Gly-Asp (RGD), a well-known characteristic of the disintegrin family. Molecular mass of jerdonin was determined to be 7483Da by matrix-assisted laser desorption ionization time of flight mass spectrometry. Jerdonin inhibited ADP- and collagen-induced human platelet aggregation with IC(50) of 220 and 240 nM, respectively. In vivo, jerdonin inhibited the growth of subcutaneously inoculated B16 solid tumor in C57BL/6 mice and improved the survival time of the tumor-bearing mice.
authors.affiliation: Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223 Yunnan, China. zhouxingding@vip.sina.com
authors.forename: Xing-Ding
authors.initials: XD
authors.lastname: Zhou
authors.forename: Yang
authors.initials: Y
authors.lastname: Jin
authors.forename: Run-Qiang
authors.initials: RQ
authors.lastname: Chen
authors.forename: Qiu-Min
authors.initials: QM
authors.lastname: Lu
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Wu
authors.forename: Wan-Yu
authors.initials: WY
authors.lastname: Wang
authors.forename: Yu-Liang
authors.initials: YL
authors.lastname: Xiong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D003435:Crotalid Venoms / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D019483:Disintegrins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D008546:Melanoma, Experimental / Q000188:drug therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D017385:Sequence Homology; D015996:Survival Rate; D017841:Trimeresurus
chemical_list: D000970:Antineoplastic Agents; D003435:Crotalid Venoms; D018076:DNA, Complementary; D019483:Disintegrins; D009842:Oligopeptides; C029748:Trimeresurus venoms; C485032:jerdonin; C047981:arginyl-glycyl-aspartic acid