title: Determination of nucleic acids with tetra-(N-hexadecylpyridiniumyl) porphyrin sensitized by cetyltrimethylammonium bromide (CTMAB) using a Rayleigh light-scattering technique.
abstract: Using a common spectrofluorometer to measure the intensity of Rayleigh light-scattering (RLS), a method for determination of nucleic acids has been developed. At pH 10.24 and ionic strength 0.01 mol l-1 (NaCl), the Rayleigh light-scattering of the tetra-(N-hexadecylpyridiniumyl) porphyrin (TC16PyP) is greatly enhanced by nucleic acids in the presence of cetyltrimethylammonium bromide (CTMAB), with the scattering peak located at 311.8 nm. The enhanced RLS intensity is in proportion to the concentration of calf thymus DNA (ctDNA) in the range 0.2-6.0 microg ml-1 and to that of fish sperm DNA (fsDNA) in the range 0.05-3.0microg ml-1. The limits of detection are 0.016 microg ml-1 for calf thymus DNA and 0.023 microg ml-1 for fish sperm DNA when the concentration of TPP was chosen 2.0 x 10(-6) mol l-1. Four synthetic samples were determined satisfactorily.
authors.affiliation: College of Chemistry and Materials Science, Anhui Normal Unversity, Wuhu 241000, PR China. zhucq625@163.com
authors.forename: Changqing
authors.initials: C
authors.lastname: Zhu
authors.forename: Shujuan
authors.initials: S
authors.lastname: Zhuo
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Li
authors.forename: Lun
authors.initials: L
authors.lastname: Wang
authors.forename: Danhua
authors.initials: D
authors.lastname: Zhao
authors.forename: Jinlong
authors.initials: J
authors.lastname: Chen
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000818:Animals; D002138:Calibration; D002417:Cattle; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry* / Q000494:pharmacology; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D003902:Detergents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005399:Fishes; D006863:Hydrogen-Ion Concentration; D007477:Ions; D008027:Light; D008297:Male; D008956:Models, Chemical; D009696:Nucleic Acids / Q000032:analysis* / Q000737:chemistry; D011166:Porphyrins / Q000032:analysis / Q000737:chemistry*; D011726:Pyridinium Compounds / Q000032:analysis / Q000737:chemistry*; D012542:Scattering, Radiation; D012965:Sodium Chloride / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*; D013094:Spermatozoa / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism
chemical_list: D002593:Cetrimonium Compounds; D003902:Detergents; D007477:Ions; D009696:Nucleic Acids; D011166:Porphyrins; D011726:Pyridinium Compounds; C492642:tetra-(N-hexadecylpyridiniumyl) porphyrin; D012965:Sodium Chloride; D004247:DNA; D000077286:Cetrimonium