Acrylamide does not cause apparent changes in genetic expression of creatine kinase in rat cerebellum.
Acrylamide inhibits creatine kinase (CK) activities in the brain of rats or mice. However, its effects on genetic expression of CK have not yet been studied. CK mRNA and protein level were examined by RT-PCR and Western blotting, respectively. Neither cytosolic CK (B subunit) and mitochondrial CK (ubiquitous form) mRNA nor B subunit protein was clearly changed in the cerebellum from rats intoxicated with acrylamide (50 mg/kg/day i.p. for 8 days) and showing clinical neurotoxic signs.
Wispriyono
Bambang
B
Department of Environmental Toxicology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
Matsuoka
Masato
M
Igisu
Hideki
H
D020106:Acrylamide / Q000633:toxicity*; D000818:Animals; D015153:Blotting, Western; D002268:Carboxypeptidases; D002531:Cerebellum / Q000201:enzymology*; D003402:Creatine Kinase / Q000235:genetics*; D003600:Cytosol / Q000201:enzymology; D015870:Gene Expression / Q000187:drug effects*; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D011506:Proteins / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
D011506:Proteins; D012333:RNA, Messenger; D020106:Acrylamide; D003402:Creatine Kinase; D002268:Carboxypeptidases; C042458:carboxypeptidase K