title: A nanoliter-scale nucleic acid processor with parallel architecture.
abstract: The purification of nucleic acids from microbial and mammalian cells is a crucial step in many biological and medical applications. We have developed microfluidic chips for automated nucleic acid purification from small numbers of bacterial or mammalian cells. All processes, such as cell isolation, cell lysis, DNA or mRNA purification, and recovery, were carried out on a single microfluidic chip in nanoliter volumes without any pre- or postsample treatment. Measurable amounts of mRNA were extracted in an automated fashion from as little as a single mammalian cell and recovered from the chip. These microfluidic chips are capable of processing different samples in parallel, thereby illustrating how highly parallel microfluidic architectures can be constructed to perform integrated batch-processing functionalities for biological and medical applications.
authors.forename: Jong Wook
authors.initials: JW
authors.lastname: Hong
authors.forename: Vincent
authors.initials: V
authors.lastname: Studer
authors.forename: Giao
authors.initials: G
authors.lastname: Hang
authors.forename: W French
authors.initials: WF
authors.lastname: Anderson
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Quake
mesh_terms: D000818:Animals; D001331:Automation; D004247:DNA / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005821:Genetic Techniques; D051379:Mice; D041681:NIH 3T3 Cells; D036103:Nanotechnology / Q000379:methods*; D009696:Nucleic Acids / Q000737:chemistry* / Q000302:isolation & purification*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D009841:Oligonucleotides / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D009696:Nucleic Acids; D009841:Oligonucleotides; D012333:RNA, Messenger; D004247:DNA