No evidence of correlation between p53 codon 72 polymorphism and risk of bladder or breast carcinoma in Tunisian patients.
The TP53 gene, frequently mutated in human cancers, carries several polymorphisms. The one most informative and studied concerns codon 72; a single base changes the CGC (arginine) to CCC (proline). The arginine form was considered to be a significant risk factor in the development of cancer. However, various reports on this polymorphism are controversial. We carried out the same investigation in two groups of patients, a group with bladder cancer and another with breast cancer, and in healthy controls in two regions of our country, using an improved PCR-RFLP method. The number of Arg/Arg, Arg/Pro, and Pro/Pro genotypes was as follows: 21, 23, 3 and 13, 19, 2 for patients (total 47) and controls (34), respectively, in the first group; 18, 9, 3 and 19, 26, 4 for patients (30) and controls (49), respectively, in the second group. Statistical analysis of the genotype and allele frequencies did not reveal any difference between patients and controls in both groups except for a weak difference between the homozygotes to heterozygotes in the second group with a chi square of 4.1 (P = 0.045); the number of breast cancer patients is actually low (30) and should be increased in order to assess such a conclusion. Our overall results are therefore not consistent with a high risk associated with TP53 codon 72 polymorphism in breast and in bladder cancers.
Mabrouk
Imed
I
Centre de Biotechnologie de Sfax, BP K 3038, Sfax, Tunisia.
Baccouche
Sami
S
El-Abed
Rym
R
Mokdad-Gargouri
Raja
R
Mosbah
Ali
A
Saïd
Salem
S
Daoud
Jamel
J
Frikha
Mounir
M
Jlidi
Rachid
R
Gargouri
Ali
A
D019943:Amino Acid Substitution; D001483:Base Sequence; D001943:Breast Neoplasms / Q000453:epidemiology / Q000235:genetics*; D003062:Codon / Q000235:genetics*; D017931:DNA Primers; D005260:Female; D016158:Genes, p53; D006580:Genetic Carrier Screening; D006720:Homozygote; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012016:Reference Values; D015183:Restriction Mapping; D018570:Risk Assessment; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D014416:Tunisia; D001749:Urinary Bladder Neoplasms / Q000453:epidemiology / Q000235:genetics*
D003062:Codon; D017931:DNA Primers; D016159:Tumor Suppressor Protein p53