title: [Effects of Angelica sinensis polysaccharides on hepatic drug metabolism enzymes activities in mice].
abstract: OBJECTIVE
  To study the effects of Angelica sinensis Polysaccharides (ASP) on the hepatic drug metabolism enzymes activities in normal mice and those prednisolone (PSL)-induced liver injury.
  
  
  METHODS
  The activities of phase II enzymes (GSH-related enzymes) and cytochrome P450 enzymes were measured by biochemical method.
  
  
  RESULTS
  ASP increased the activities of glutathione S-transferase in liver microsomes and mitochondria. The cytochrome P450 content, NADPH-cytochrome c reductase, aminopyrine N-demethylase, and aniline hydroxylase activities in liver microsomes were also increased. PSL significantly increased serum ALT levels, and decreased the liver mitochondrial glutathione content. At the same time, other enzymes activities were all increased. When mice were treated with ASP 2.0 g.kg-1, the PSL-induced changes on cytochrome P450 enzymes, glutathione S-transferase, and GSH content were restored.
  
  
  CONCLUSIONS
  ASP can modulate the activities of drug metabolism enzymes.
authors.affiliation: Department of Pharmacology, School of Medicine, Wuhan University, Wuhan 430071, HuBei, China.
authors.forename: Xue-yan
authors.initials: XY
authors.lastname: Xia
authors.forename: Ren-xiu
authors.initials: RX
authors.lastname: Peng
authors.forename: Rui
authors.initials: R
authors.lastname: Kong
authors.forename: Zhe-qiong
authors.initials: ZQ
authors.lastname: Yang
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
mesh_terms: D000633:Aminopyrine N-Demethylase / Q000378:metabolism; D029971:Angelica sinensis / Q000737:chemistry*; D000815:Aniline Hydroxylase / Q000378:metabolism; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology* / Q000209:etiology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D011239:Prednisolone
chemical_list: D011134:Polysaccharides; D003577:Cytochrome P-450 Enzyme System; D011239:Prednisolone; D000815:Aniline Hydroxylase; D000633:Aminopyrine N-Demethylase; D009251:NADPH-Ferrihemoprotein Reductase; D005982:Glutathione Transferase