title: Mean nuclear volume in ovarian papillary serous cystadenocarcinoma.
abstract: OBJECTIVE
  To measure mean nuclear volume (MNV), estimated by the stereologic intercept method, in ovarian serous carcinomas and to compare that between omentum-positive cases (cases with omental metastasis) and -negative cases.
  
  
  METHODS
  This retrospective study consisted of 29 cases (stage I and II = 13 cases, and stage IIIb = 16 cases) of ovarian papillary serous cystadenocarcinoma. MNV of all cases (at primary and metastatic sites) was estimated with an image cytometer. The nuclear point intersection method was used to measure nuclear volume. Mean intercepted diameters of at least 50 nuclei were measured. MNV was correlated with histologic grade, International Federation of Gynecology and Obstetrics (FIGO) staging of ovarian malignancy and primary tumor versus metastatic deposits.
  
  
  RESULTS
  MNVs estimated in omentum-negative (FIGO stage I and II) and omentum-positive (FIGO stage IIIb) cases were 1,022.48 +/- 608.45 and 2,152 +/- 1,317.51 microns 3 (P = .05, Student t test), respectively. Significant differences in MNV were also observed among the different grades of tumor. However, no significant difference was observed between MNVs of primary and metastatic tumors in omentum-positive cases.
  
  
  CONCLUSIONS
  Estimates of MNV on conventional histopathology sections may provide objective and useful criteria for relatively subjective histopathology grading and staging (FIGO stage I and II vs. IIIb).
authors.affiliation: Departments of Pathology, Cytology, and Gynecology and Obstetrics, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: Sambit K
authors.initials: SK
authors.lastname: Mohanty
authors.forename: Pranab
authors.initials: P
authors.lastname: Dey
authors.forename: Subhash C
authors.initials: SC
authors.lastname: Saha
mesh_terms: D002467:Cell Nucleus / Q000473:pathology*; D018284:Cystadenocarcinoma, Serous / Q000473:pathology*; D003627:Data Interpretation, Statistical; D004801:Eosine Yellowish-(YS) / Q000737:chemistry; D005260:Female; D006416:Hematoxylin / Q000737:chemistry; D006801:Humans; D019044:Image Cytometry / Q000379:methods; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D009852:Omentum / Q000473:pathology; D010051:Ovarian Neoplasms / Q000473:pathology*; D010534:Peritoneal Neoplasms / Q000473:pathology
chemical_list: D004801:Eosine Yellowish-(YS); D006416:Hematoxylin