title: Comparison of post-translational modifications of alpha A-crystallin from normal and hereditary cataract rats.
abstract: In order to investigate the relationship between lens opacities and the various modifications of lens proteins, we analyzed and compared the properties of lens proteins of 85-day old normal Wistar rats and the hereditary cataract model, ICR/f rats. The present study identified many differences between normal and mutant lens proteins. In the ICR/f mutant rats, the relative amounts of gamma-crystallin decreased and high molecular weight (HMW) protein increased. Racemization and isomerization of Asp-151 of alpha A-crystallin was observed in the mutant ICR/f rats, and Met-1 of alpha A-crystallin was oxidized to methionine sulfoxide. These modifications were not found in the age-matched normal rats. These tendencies are consistent with aged and cataractous human lenses.
authors.affiliation: Research Reactor Institute, Kyoto University, Kumatori, Sennan, Osaka, Japan. nfujii@HL.rri.kyoto-u.ac.jp
authors.forename: N
authors.initials: N
authors.lastname: Fujii
authors.forename: N
authors.initials: N
authors.lastname: Takeuchi
authors.forename: N
authors.initials: N
authors.lastname: Fujii
authors.forename: T
authors.initials: T
authors.lastname: Tezuka
authors.forename: K
authors.initials: K
authors.lastname: Kuge
authors.forename: T
authors.initials: T
authors.lastname: Takata
authors.forename: A
authors.initials: A
authors.lastname: Kamei
authors.forename: T
authors.initials: T
authors.lastname: Saito
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D002386:Cataract / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D006801:Humans; D007536:Isomerism; D007908:Lens, Crystalline / Q000737:chemistry / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry; D011499:Protein Processing, Post-Translational; D051381:Rats; D011922:Rats, Mutant Strains; D017208:Rats, Wistar; D013997:Time Factors; D038202:alpha-Crystallin A Chain / Q000737:chemistry* / Q000378:metabolism* / Q000502:physiology
chemical_list: D010455:Peptides; D038202:alpha-Crystallin A Chain; D001224:Aspartic Acid; D008715:Methionine