Renal expression of osmotically responsive cation channel TRPV4 is restricted to water-impermeant nephron segments.
TRPV4, a nonselective cation channel of the transient receptor potential (TRP) family, is gated by hypotonicity. Expression of TRPV4 mRNA has been detected in the circumventricular organs of the brain responsible for sensing systemic tonicity and in the kidney distal convoluted tubule (DCT), among other sites. No analysis of TRPV4 expression at the protein level has been undertaken and no systematic analysis of expression of this channel has been reported in the kidney. Via RNAse protection assay and immunoblotting, abundant expression of TRPV4 was detected in the cortex, medulla, and papilla. The expression pattern of TRPV4 was characterized in both rat and mouse kidney, which revealed similar patterns of immunoreactivity. TRPV4 expression was absent from the proximal tubule (PT) and descending thin limb (DTL), whereas the strongest expression was observed in the ascending thin limb (ATL). The thick ascending limb (TAL) was strongly positive as was the DCT and connecting tubule. Importantly, the water-permeant cells of the macula densa were unstained. Moderate TRPV4 expression was noted in all collecting duct portions and in papillary epithelium; intercalated cells (type A) exhibited a particularly strong signal. In all positive segments, TRPV4 expression was concentrated at the basolateral membrane. Therefore, TRPV4 is expressed in only those nephron segments that are constitutively (i.e., ATL, TAL, and DCT) or conditionally (i.e., collecting duct) water impermeant and where generation of a substantial transcellular osmotic gradient could be expected. TRPV4 expression is absent from nephron segments exhibiting constitutive water permeability and unregulated apical aquaporin expression (i.e., PT and DTL). These data, although circumstantial, are consistent with a role for TRPV4 in the response to anisotonicity in the mammalian kidney.
Tian
Wei
W
Division of Nephrology and Hypertension, Oregon Health and Science University, Portland Veterans Affairs Medical Center, Portland, OR 97239, USA.
Salanova
Michele
M
Xu
Hongshi
H
Lindsley
Jessie N
JN
Oyama
Terry T
TT
Anderson
Sharon
S
Bachmann
Sebastian
S
Cohen
David M
DM
D000818:Animals; D027682:Cation Transport Proteins / Q000096:biosynthesis* / Q000494:pharmacology*; D004594:Electrophysiology; D005786:Gene Expression Regulation; D017403:In Situ Hybridization; D007473:Ion Channels / Q000096:biosynthesis* / Q000494:pharmacology*; D008297:Male; D009399:Nephrons / Q000502:physiology*; D009997:Osmotic Pressure; D010539:Permeability; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D050916:TRPV Cation Channels; D014867:Water / Q000378:metabolism; D014882:Water-Electrolyte Balance / Q000502:physiology*
D027682:Cation Transport Proteins; D007473:Ion Channels; D050916:TRPV Cation Channels; C486576:Trpv4 protein, mouse; C486577:Trpv4 protein, rat; D014867:Water