P-15 cell-binding domain derived from collagen: analysis of MG63 osteoblastic-cell response by means of a microarray technology.
BACKGROUND
P-15 is an analog of the cell-binding domain of collagen. P-15 has been shown to facilitate physiological processes in a way similar to collagen; to serve as an anchorage for cells; and to promote the binding, migration, and differentiation of cells.


METHODS
Expression profiling by DNA microarray is a molecular technology that allows the analysis of gene expression in a cell system. By using DNA microarrays containing 19,200 genes, we identified in osteoblast-like cell line (MG-63) cultured with P-15 several genes whose expression was significantly up- or downregulated.


RESULTS
The differentially expressed genes cover a broad range of functional activities: 1) signaling transduction, 2) differentiation, 3) apoptosis, and 4) cell-cycle regulation. It was also possible to detect some genes whose function is unknown.


CONCLUSIONS
The data reported are, to our knowledge, the first genetic portrait of P-15 effects. They can help us to better understand the molecular mechanism of osteogenesis and can serve as a model for comparing different cell cultures and/or other materials with similar effect.
Carinci
Francesco
F
Department of Maxillofacial Surgery, University of Ferrara, Ferrara, Italy.
Pezzetti
Furio
F
Volinia
Stefano
S
Laino
Gregorio
G
Arcelli
Diego
D
Caramelli
Elisabetta
E
Degidi
Marco
M
Piattelli
Adriano
A
D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D003094:Collagen / Q000494:pharmacology*; D024042:Collagen Type I / Q000737:chemistry / Q000502:physiology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D005796:Genes / Q000187:drug effects; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics*; D010446:Peptide Fragments / Q000494:pharmacology*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D015398:Signal Transduction / Q000187:drug effects
D024042:Collagen Type I; D010446:Peptide Fragments; C407062:cell-binding peptide P-15; D003094:Collagen