title: In vivo topical anti-inflammatory and in vitro antioxidant activities of two extracts of Thymus satureioides leaves.
abstract: Four extracts at increasing polarity were prepared from the leaves of Thymus satureioides Coss. (Labiatae) and assayed for the in vivo topical anti-inflammatory effect using the croton oil ear test in mice, and for in vitro both antioxidant (DPPH degrees test) and anti-bacterial (broth microdilution method) activities. The chloroform extract showed a topical anti-inflammatory activity (ID50=282 microg cm(-2)), only three times lower than that of the reference drug indomethacin (ID50=93 microg cm(-2)) and its active components were identified as ursolic and oleanolic acids. The methanol extract, showing a significant radical-scavenging effect (SC50=14.54 microg), was characterized by the isolation and identification of some flavonoids. On the contrary, the extracts did not show any anti-bacterial effect against four standard aerobial bacteria strains.
authors.affiliation: Faculté de Médecine et Pharmacie, Université Mohammed V, Rabat, Morocco.
authors.forename: H
authors.initials: H
authors.lastname: Ismaili
authors.forename: L
authors.initials: L
authors.lastname: Milella
authors.forename: S
authors.initials: S
authors.lastname: Fkih-Tetouani
authors.forename: A
authors.initials: A
authors.lastname: Ilidrissi
authors.forename: A
authors.initials: A
authors.lastname: Camporese
authors.forename: S
authors.initials: S
authors.lastname: Sosa
authors.forename: G
authors.initials: G
authors.lastname: Altinier
authors.forename: R
authors.initials: R
authors.lastname: Della Loggia
authors.forename: R
authors.initials: R
authors.lastname: Aquino
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D007249:Inflammation / Q000188:drug therapy*; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; D014315:Triterpenes