title: MK2-induced tristetraprolin:14-3-3 complexes prevent stress granule association and ARE-mRNA decay.
abstract: Stress granules (SGs) are dynamic cytoplasmic foci at which stalled translation initiation complexes accumulate in cells subjected to environmental stress. SG-associated proteins such as TIA-1, TIAR and HuR bind to AU-rich element (ARE)-containing mRNAs and control their translation and stability. Here we show that tristetraprolin (TTP), an ARE-binding protein that destabilizes ARE-mRNAs, is recruited to SGs that are assembled in response to FCCP-induced energy deprivation, but not arsenite-induced oxidative stress. Exclusion of TTP from arsenite-induced SGs is a consequence of MAPKAP kinase-2 (MK2)-induced phosphorylation at serines 52 and 178, which promotes the assembly of TTP:14-3-3 complexes. 14-3-3 binding excludes TTP from SGs and inhibits TTP-dependent degradation of ARE-containing transcripts. In activated RAW 264.7 macrophages, endogenous TTP:14-3-3 complexes bind to ARE-RNA. Our data reveal the mechanism by which the p38-MAPK/MK2 kinase cascade inhibits TTP-mediated degradation of ARE-containing transcripts and thereby contributes to lipopolysaccharide-induced TNFalpha expression.
authors.affiliation: Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Georg
authors.initials: G
authors.lastname: Stoecklin
authors.forename: Tiffany
authors.initials: T
authors.lastname: Stubbs
authors.forename: Nancy
authors.initials: N
authors.lastname: Kedersha
authors.forename: Stephen
authors.initials: S
authors.lastname: Wax
authors.forename: William F C
authors.initials: WF
authors.lastname: Rigby
authors.forename: T Keith
authors.initials: TK
authors.lastname: Blackwell
authors.forename: Paul
authors.initials: P
authors.lastname: Anderson
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism*; D000241:Adenosine / Q000235:genetics; D000818:Animals; D018053:Arsenites / Q000494:pharmacology; D001665:Binding Sites; D002460:Cell Line; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004789:Enzyme Activation; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D020935:MAP Kinase Signaling System; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D009154:Mutation / Q000235:genetics; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D038621:Regulatory Sequences, Ribonucleic Acid / Q000235:genetics*; D012694:Serine / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D051816:Tristetraprolin; D014529:Uridine / Q000235:genetics; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism; D000099190:MAP-Kinase-Activated Kinase 2
chemical_list: D048948:14-3-3 Proteins; D000241:Adenosine; D018053:Arsenites; D004268:DNA-Binding Proteins; D017874:Immediate-Early Proteins; D047908:Intracellular Signaling Peptides and Proteins; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D038621:Regulatory Sequences, Ribonucleic Acid; D012694:Serine; D051816:Tristetraprolin; D014529:Uridine; D048051:p38 Mitogen-Activated Protein Kinases; D000099190:MAP-Kinase-Activated Kinase 2; C497552:ZFP36 protein, human; C497553:Zfp36 protein, mouse; C015001:arsenite