title: Uridine 5'-polyphosphates (p4U and p5U) and uridine(5')polyphospho(5')nucleosides (Up(n)Ns) can be synthesized by UTP:glucose-1-phosphate uridylyltransferase from Saccharomyces cerevisiae.
abstract: UTP:glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) from Saccharomyces cerevisiae can transfer the uridylyl moiety from UDP-glucose onto tripolyphosphate (P(3)), tetrapolyphosphate (P(4)), nucleoside triphosphates (p(3)Ns) and nucleoside 5'-polyphosphates (p(4)Ns) forming uridine 5'-tetraphosphate (p(4)U), uridine 5'-pentaphosphate (p(5)U) and dinucleotides, such as Ap(4)U, Cp(4)U, Gp(4)U, Up(4)U, Ap(5)U and Gp(5)U. Unlike UDP-glucose, UDP-galactose was not a UMP donor and ADP was not a UMP acceptor. This is the first example of an enzyme that may be responsible for accumulation of dinucleoside tetraphosphates containing two pyrimidine nucleosides in vivo. Occurrence of such dinucleotides in S. cerevisiae and Escherichia coli has been previously reported (Coste et al., J. Biol. Chem. 262 (1987) 12096-12103).
authors.affiliation: Departamento de Bioquímica, Instituto de Investigaciones Biomédicas Alberto Sols, UAM/CSIC, Facultad de Medicina, c. Arzobispo Morcillo 4, 28029 Madrid, Spain. guranow@au.poznan.pl
authors.forename: Andrzej
authors.initials: A
authors.lastname: Guranowski
authors.forename: Anabel
authors.initials: A
authors.lastname: de Diego
authors.forename: Antonio
authors.initials: A
authors.lastname: Sillero
authors.forename: María Antonia
authors.initials: MA
authors.lastname: Günther Sillero
mesh_terms: D015226:Dinucleoside Phosphates / Q000096:biosynthesis*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase / Q000378:metabolism*; D014500:Uracil Nucleotides / Q000096:biosynthesis*; D014531:Uridine Diphosphate Galactose / Q000378:metabolism; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
chemical_list: D015226:Dinucleoside Phosphates; D029701:Saccharomyces cerevisiae Proteins; D014500:Uracil Nucleotides; C035436:uridine 5'-tetraphosphate; D014531:Uridine Diphosphate Galactose; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase; D014532:Uridine Diphosphate Glucose