Cestrum yellow leaf curling virus (CmYLCV) promoter: a new strong constitutive promoter for heterologous gene expression in a wide variety of crops.
Appropriately regulated gene expression requires a suitable promoter. A number of promoters have been isolated and shown to be functional in plants, but only a few of them activate transcription of transgenes at high levels constitutively. We report here the cloning and characterization of a novel, constitutively expressed promoter isolated from Cestrum yellow leaf curling virus (CmYLCV), a double-stranded DNA plant pararetrovirus belonging to the Caulimoviridae family. The CmYLCV promoter is highly active in callus, meristems and vegetative and reproductive tissues in Arabidopsis thaliana, Nicotiana tabacum, Lycopersicon esculentum, Zea mays and Oryza sativa. Furthermore, the level of expression is comparable to, or higher than, that from the CaMV 35S, the 'super-promoter' or the maize ubiquitin 1 promoters, three frequently used promoters in agricultural biotechnology. The heritable, strong and constitutive activity in both monocotyledonous and dicotyledonous plants, combined with the extremely narrow CmYLCV host range, makes the CmYLCV promoter an attractive tool for regulating transgene expression in a wide variety of plant species.
Stavolone
Livia
L
Friedrich Miescher Institute, Maulbeerstrasse 66, 4058 Basel, Switzerland. l.stavolone@area.ba.cnr.it
Kononova
Maria
M
Pauli
Sandra
S
Ragozzino
Antonio
A
de Haan
Peter
P
Milligan
Steve
S
Lawton
Kay
K
Hohn
Thomas
T
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D017796:Caulimovirus / Q000235:genetics*; D032301:Cestrum / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay; D018628:Gene Dosage; D015870:Gene Expression; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins; D006651:Histocytochemistry; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D012275:Oryza / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D019076:Transgenes / Q000235:genetics; D003313:Zea mays / Q000235:genetics / Q000378:metabolism
D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D005966:Glucuronidase