Flow calorimetry--a useful tool for determination of immobilized cis-epoxysuccinate hydrolase activity from Nocardia tartaricans.
Bacterial cells Nocardia tartaricans with cis-epoxysuccinate hydrolase activity were entrapped in hardened calcium pectate gel by a commercial high performance encapsulator. This enzyme (in a single step reaction with no formation of side products) was used to hydrolyze disodium cis-epoxysuccinate to a pure enantiomer--disodium L-(+)-tartrate. Activities of this enzyme were determined using flow calorimetry. The validity of this method was corroborated by HPLC and isotachophoresis. The immobilized biocatalyst has activity (75.8 U/mgdry) able to convert disodium cis-epoxysuccinate to disodium tartrate at 94% yield in 5.5h. Immobilization of N. tartaricans in hardened calcium pectate gel beads had a positive effect on the activity of cis-epoxysuccinate hydrolase, storage stability, yield, and time of bioconversion.
Vikartovská
Alica
A
Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Bucko
Marek
M
Gemeiner
Peter
P
Nahálka
Jozef
J
Pätoprstý
Vladimir
V
Hrabárová
Eva
E
D002151:Calorimetry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D006867:Hydrolases / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008863:Microspheres; D009618:Nocardiaceae / Q000201:enzymology*; D010368:Pectins; D013386:Succinates / Q000378:metabolism; D013644:Tartrates / Q000032:analysis / Q000138:chemical synthesis
D004800:Enzymes, Immobilized; D013386:Succinates; D013644:Tartrates; C017290:2,3-epoxysuccinic acid; D010368:Pectins; D006867:Hydrolases; C492296:cis-epoxysuccinate hydrolase, Nocardia tartaricans; C003181:polygalacturonic acid; C029768:tartaric acid