Nitrogen catabolite repression in Saccharomyces cerevisiae during wine fermentations.
We carried out fermentations with several nitrogen sources in different concentrations and studied nitrogen regulation by following the transcriptional profile of the general amino-acid permease (GAP1) and the ammonium permeases (MEP1, MEP2, MEP3). In wine fermentations the cells evolve from a nitrogen-repressed situation at the beginning of the process to a nitrogen-derepressed situation as the nitrogen is consumed. These nitrogen-repressed/derepressed conditions determined the different patterns of ammonium and amino-acid consumption. Arginine and alanine were hardly used under the repressed conditions, while the uptake of branched-chain and aromatic amino acids increased.
Beltran
Gemma
G
Unitat d'Enologia del Centre de Referència de Tecnologia d'Aliments, Dept. Bioquímica i Biotecnologia, Facultat d'Enologia de Tarragona, Universitat Rovira i Virgili, Ramón y Cajal, 70, 43005 Tarragona, Spain.
Novo
Maite
M
Rozès
Nicolas
N
Mas
Albert
A
Guillamón
José M
JM
D026905:Amino Acid Transport Systems / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D027682:Cation Transport Proteins / Q000378:metabolism; D005285:Fermentation; D015966:Gene Expression Regulation, Fungal; D009584:Nitrogen / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D014920:Wine / Q000382:microbiology*
D026905:Amino Acid Transport Systems; D000596:Amino Acids; D027682:Cation Transport Proteins; C088951:MEP1 protein, S cerevisiae; D000644:Quaternary Ammonium Compounds; D029701:Saccharomyces cerevisiae Proteins; D009584:Nitrogen