Selective deposition of native cell membranes on biocompatible micropatterns.
We establish two methods to deposit native biomembranes (human erythrocyte membranes and sarcoplasmic reticulum membranes) selectively onto biocompatible microtemplates. The first method utilizes UV photolithography to micropattern the regenerated cellulose, while the second uses the "stamping" of protein barriers onto homogeneous cellulose supports. The relatively simple methods established here allow for the position selective spreading of three-dimensional native cells into two-dimensional films, retaining the orientation and lateral density of transmembrane proteins in their native state.
Tanaka
Motomu
M
Lehrstuhl für Biophysik E22, Technische Universität München, James-Franck-Strasse 1, D-85748 Garching, Germany. mtanaka@ph.tum.de
Wong
Amy P
AP
Rehfeldt
Florian
F
Tutus
Murat
M
Kaufmann
Stefan
S
D001672:Biocompatible Materials / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D004910:Erythrocyte Membrane / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives* / Q000737:chemistry; D005453:Fluorescence; D005898:Glass / Q000737:chemistry; D006801:Humans; D012519:Sarcoplasmic Reticulum / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry
D001672:Biocompatible Materials; C039217:fluorescein isothiocyanate bovine serum albumin; D012710:Serum Albumin, Bovine; D002482:Cellulose; D016650:Fluorescein-5-isothiocyanate