Acute toxic effect of the algal yessotoxin on Purkinje cells from the cerebellum of Swiss CD1 mice.
Swiss CD1 mice died less than 2 h after intraperitoneal injection of 420 microg/kg of algal yessotoxin (YTX). The morphological, histochemical and immunocytochemical studies performed on the cerebellar cortex revealed damage to the Purkinje cells. The main cytological alterations were observed in the cytoplasm, while less sufferance was detected in the nucleus. The immunocytochemical experiments showed an increased positivity to S100 protein while there was a decreased response to calbindin D-28K, beta-tubulin and neurofilaments. These changes in intracellular Ca(2+)-binding proteins and the modifications in the cytoskeletal components of Purkinje cells suggest that YTX may be involved in neurological disorders.
Franchini
A
A
Department of Animal Biology, University of Modena and Reggio Emilia, Via Campi 213/D, Modena 41100, Italy.
Marchesini
E
E
Poletti
R
R
Ottaviani
E
E
D000818:Animals; D064026:Calbindins; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002525:Cerebellar Cortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D004988:Ethers, Cyclic / Q000633:toxicity*; D056890:Eukaryota; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008978:Mollusk Venoms; D009498:Neurotoxins / Q000633:toxicity*; D035964:Oxocins / Q000633:toxicity*; D011689:Purkinje Cells / Q000187:drug effects*; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
D064026:Calbindins; D002135:Calcium-Binding Proteins; D004988:Ethers, Cyclic; D008978:Mollusk Venoms; D009498:Neurotoxins; D035964:Oxocins; D064030:S100 Calcium Binding Protein G; D014404:Tubulin; C066632:yessotoxin