Protein expression clone repositories for functional proteomics.
The information generated from the sequence of the human genome has inspired efforts to systematically develop organized collections of human cDNA clones for use in expression screens in mammalian cells. These high-throughput cloning initiatives offer significant advantages over the cDNA libraries that have been used in the past, including greater experimental flexibility, immediate identification of hits, information regarding all tested proteins (even for those giving no response) and eventually more comprehensive coverage. Some of the lessons learned and the considerations that underlie the creation of genome-wide cDNA repositories are discussed here. Although still inchoate, these resources are already impacting the manner in which high-throughput functional screens are performed.
Pearlberg
Joseph
J
Harvard Institute of Proteomics, and Department of Biochemistry and Molecular Biology, Harvard Medical School, Boston, MA, USA.
LaBaer
Joshua
J
D000818:Animals; D018070:Biological Specimen Banks; D003001:Cloning, Molecular / Q000379:methods; D019295:Computational Biology; D006801:Humans; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D040901:Proteomics / Q000295:instrumentation* / Q000379:methods; D011786:Quality Control
D011506:Proteins