Response of the Pacific oyster Crassostrea gigas to hydrocarbon contamination under experimental conditions.
Hydrocarbon contamination perturbs the metabolism of the marine bivalve Crassostrea gigas. To understand the response of this organism to hydrocarbon exposure, a suppression subtractive hybridisation method was employed to characterise up- and down-regulated genes during hydrocarbon exposure. The number of differentially expressed gene sequences obtained via this method was 258. The expression of genes involved in hydrocarbon detoxification (cytochrome p4501A1-like protein, cytochrome b(5), flavin-containing monooxygenase 2 and glutathione S-transferase omega class), protection against oxidative stress (copper/zinc superoxide dismutase) and cell protection (heat shock protein 70 family) was analysed by RT-PCR. An increase in the mRNA level of all genes studied was observed. A quantification of HSP70 by Enzyme Linked Immunosorbent Assay (ELISA) showed a significant increase of this protein during exposure. This study provides a basis for studying hydrocarbon detoxification processes in marine bivalves, especially C. gigas.
Boutet
Isabelle
I
Laboratoire des Sciences de l'Environnement Marin (LEMAR), UMR-CNRS 6539, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, Tech. Brest Iroise, Place Nicolas Copernic, 29280 Plouzané, France.
Tanguy
Arnaud
A
Moraga
Dario
D
D000818:Animals; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005648:Fuel Oils / Q000032:analysis / Q000633:toxicity; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006838:Hydrocarbons / Q000032:analysis / Q000633:toxicity*; D010125:Ostreidae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013482:Superoxide Dismutase / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
D018076:DNA, Complementary; D005648:Fuel Oils; D018840:HSP70 Heat-Shock Proteins; D006838:Hydrocarbons; D014874:Water Pollutants, Chemical; D012313:RNA; D004247:DNA; D013482:Superoxide Dismutase