In vitro study of a neodynium:yttrium aluminum perovskite laser on human nonexposed pulp after cavity preparation.
OBJECTIVE
The aim of this study was to investigate dental pulp reactions after a neodynium:yttrium aluminum perovskite laser pulse on the dentinal floor of occlusal cavities in an in vitro model.


METHODS
A Lokki dt laser was used at 30 Hz, 5 W, and 160 mJ for 0.5 s. The pulp reactions were analyzed in a previously described human tooth slice cultured model. The following markers were identified by immunohistochemistry: collagens I, III, and IV and HLA-DR-positive cells.


RESULTS
After 4 days of culture, under laser pulse, a concentration of type III collagen beneath the odontoblast layer, a higher level of vessels and an accumulation of HLA-DR-positive cells were routinely observed subjacent to the cavity.


CONCLUSIONS
This laser treatment leads to the first step of rapid pulp repair under culture conditions.
Seux
Dominique
D
Laboratoire du Developpement des Tissus Dentaires, Faculté d'Odontologie, rue G. Paradin, 69372, Lyon cedex 08, France.
Roméas
Annick
A
Antoine
Bruno
B
Magloire
Henry
H
Bleicher
Françoise
F
D000293:Adolescent; D000328:Adult; D003713:Dendritic Cells / Q000276:immunology; D003737:Dental Cavity Preparation / Q000379:methods; D003782:Dental Pulp / Q000276:immunology / Q000528:radiation effects*; D024022:Fibrillar Collagens / Q000528:radiation effects; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D053685:Laser Therapy; D009354:Neodymium; D009804:Odontoblasts / Q000528:radiation effects*
D024022:Fibrillar Collagens; D006684:HLA-DR Antigens; D009354:Neodymium