Displaced granule cells in the molecular layer of the cerebellar cortex in mice treated with methylazoxymethanol.
To study the effect of a reduced granule cell number on the development of the cerebellum, mice were administered with methylazoxymethanol (MAM) at birth and examined histologically at 21 days of age. In these mice, green fluorescent protein had been knocked in within the glutamic acid decarboxylase 67 gene, resulting in fluorescent GABAergic neurons. In severe cases, granule cells were greatly reduced in number and mixed with Purkinje cells instead of forming layers. In about 10% of the MAM-treated mice, an ectopic cell layer consisting of granule cells was observed within the molecular layer. Concomitantly, basket cells disappeared. The transient interruption of granule cell production in the external granular layer presumably resulted in their incomplete migration through the molecular layer.
Yamanaka
Hajime
H
Neuronal Network Mechanisms Research Group, RIKEN Brain Science Institute, Wako, Saitama, Japan.
Obata
Kunihiko
K
D000818:Animals; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D002525:Cerebellar Cortex / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology; D005260:Female; D005968:Glutamate Decarboxylase / Q000096:biosynthesis / Q000235:genetics; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D008746:Methylazoxymethanol Acetate / Q000031:analogs & derivatives* / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D009474:Neurons / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology
D007527:Isoenzymes; D008746:Methylazoxymethanol Acetate; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1; C030667:methylazoxymethanol