Generation of Arabidopsis thaliana plants with complex N-glycans lacking beta1,2-linked xylose and core alpha1,3-linked fucose.
The plant glycosyltransferases, beta1,2-xylosyltransferase (XylT) and core alpha1,3-fucosyltransferase (FucT), are responsible for the transfer of beta1,2-linked xylose and core alpha1,3-linked fucose residues to glycoprotein N-glycans. These glycan epitopes are not present in humans and thus may cause immunological responses, which represent a limitation for the therapeutic use of recombinant mammalian glycoproteins produced in transgenic plants. Here we report the genetic modification of the N-glycosylation pathway in Arabidopsis thaliana plants. Knockout plants were generated with complete deficiency of XylT and FucT. These plants lack antigenic protein-bound N-glycans and instead synthesise predominantly structures with two terminal betaN-acetylglucosamine residues (GlcNAc(2)Man(3)GlcNAc(2)).
Strasser
R
R
Institut für Angewandte Genetik und Zellbiologie, Universität für Bodenkultur Wien, Muthgasse 18, A-1190 Wien, Austria. richard.strasser@boku.ac.at
Altmann
F
F
Mach
L
L
Glössl
J
J
Steinkellner
H
H
D000117:Acetylglucosamine; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D015153:Blotting, Western; D005643:Fucose / Q000032:analysis / Q000172:deficiency; D005647:Fucosyltransferases / Q000032:analysis / Q000172:deficiency* / Q000235:genetics; D006031:Glycosylation; D009154:Mutation; D010430:Pentosyltransferases / Q000032:analysis / Q000172:deficiency* / Q000235:genetics; D011134:Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014994:Xylose / Q000032:analysis / Q000172:deficiency
D011134:Polysaccharides; D005643:Fucose; D014994:Xylose; D005647:Fucosyltransferases; C031692:galactoside 3-fucosyltransferase; D010430:Pentosyltransferases; C408586:beta 1,2-xylosyltransferase; D000117:Acetylglucosamine