Influence of nutrients on growth and bacteriocin production by Leuconostoc mesenteroides L124 and Lactobacillus curvatus L442.
The aim of this study was to investigate the effect of complex nutrients on microbial growth and bacteriocin production, in order to improve bacteriocin synthesis during the growth cycle of Leuconostoc mesenteroides L124 and Lactobacillus curvatus L442. The fermentations were conducted at the optimum pH and temperature for bacteriocin production (pH 5.5+/-0.1 and temperature 25+/-0.1 degrees C). Because of their association with the final biomass, conditions favouring the increase of the produced biomass resulted in the increase of bacteriocin activity in the growth medium. Since the produced final biomass and the final concentration of the bacteriocins were associated with the amount of the carbon (glucose) and nitrogen source, better growth of the lactic acid bacterial strains favoured the increase of the specific bacteriocin production. Additionally, the bacteriocin production was influenced by carbon/nitrogen ratio.
Mataragas
M
M
Laboratory of Food Quality Control and Hygiene, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece.
Drosinos
E H
EH
Tsakalidou
E
E
Metaxopoulos
J
J
D001430:Bacteriocins / Q000096:biosynthesis*; D018533:Biomass; D003470:Culture Media; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000254:growth & development* / Q000378:metabolism; D007934:Leuconostoc / Q000254:growth & development* / Q000378:metabolism; D013696:Temperature
D001430:Bacteriocins; D003470:Culture Media; D005947:Glucose