title: MUC1 can interact with adenomatous polyposis coli in breast cancer.
abstract: The MUC1 tumor antigen is overexpressed on most breast tumors and metastases. It interacts with signaling proteins such as the ErbB kinases and beta-catenin, and is involved in mammary gland oncogenesis and tumor progression. Herein, we report a novel interaction between MUC1 and adenomatous polyposis coli (APC), a tumor suppressor involved in downregulating beta-catenin signaling. Initially identified in colorectal cancer, APC is also downregulated in breast tumors and presumably involved in mammary carcinogenesis. MUC1 and APC co-immunoprecipitate from the ZR-75-1 human breast carcinoma cell line and co-localize in mouse mammary glands and tumors. These studies also indicate that the association of MUC1 and APC may be increased by epidermal growth factor stimulation. Intriguingly, the co-immunoprecipitation of MUC1 and APC increases in human breast tumors and metastases as compared to adjacent normal tissues, indicating that this association may play a role in the formation and progression of breast tumors.
authors.affiliation: Tumor Biology Program, Mayo Clinic College of Medicine, Mayo Clinic, Scottsdale, AZ, USA.
authors.forename: Christine L
authors.initials: CL
authors.lastname: Hattrup
authors.forename: Julia
authors.initials: J
authors.lastname: Fernandez-Rodriguez
authors.forename: Joyce A
authors.initials: JA
authors.lastname: Schroeder
authors.forename: Gunnar C
authors.initials: GC
authors.lastname: Hansson
authors.forename: Sandra J
authors.initials: SJ
authors.lastname: Gendler
mesh_terms: D025601:Adenomatous Polyposis Coli Protein / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D006801:Humans; D042361:Mammary Glands, Human / Q000737:chemistry; D015674:Mammary Neoplasms, Animal / Q000378:metabolism; D051379:Mice; D018396:Mucin-1 / Q000032:analysis / Q000378:metabolism*; D009362:Neoplasm Metastasis; D011233:Precipitin Tests
chemical_list: D025601:Adenomatous Polyposis Coli Protein; D018396:Mucin-1; D004815:Epidermal Growth Factor