title: Acute no-effect dose for in ova exposure to C3F7 tagged 5-hydroxytryptophan, a novel probe for investigating neural development.
abstract: OBJECTIVE
  To create a probe for investigating neural development in chicks using magnetic resonance, a C(3)F(7) tag was bonded to the indole ring of 5-hydroxytryptophan (5-HTP). Immunoassays indicate that a low dose (5 microg/egg) administered in ova results in 0.5-1.0 microg accumulating in a 250 microL midbrain.
  
  
  METHODS
  Given the potential for developmental neurotoxicity of a compound that replaces an endogenous neurotransmitter, we investigated the effects of acute exposure during development.
  
  
  RESULTS
  No qualitative differences were observed between treatment groups. Differences in mortality rates between treatment groups were not statistically significant [X(2)(obs)=2.30, X(2)(crit) (df=2)=5.99, P>.05].
  
  
  CONCLUSIONS
  The no-effect dose for in ova administration of C(3)F(7) tagged5-HTP is 5 microg/egg on Day 17.
authors.affiliation: Marist College, Poughkeepsie, NY, USA. Sherry.Dingman@marist.edu
authors.forename: Sherry
authors.initials: S
authors.lastname: Dingman
authors.forename: Lara
authors.initials: L
authors.lastname: Hurlburt
authors.forename: Craig
authors.initials: C
authors.lastname: Branch
mesh_terms: D006916:5-Hydroxytryptophan / Q000633:toxicity; D000818:Animals; D002645:Chickens; D005461:Fluorine; D005466:Fluorocarbons / Q000737:chemistry / Q000633:toxicity*; D008279:Magnetic Resonance Imaging; D009420:Nervous System / Q000187:drug effects / Q000196:embryology*; D010063:Ovum / Q000187:drug effects; D012701:Serotonin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000633:toxicity; D012702:Serotonin Antagonists / Q000633:toxicity
chemical_list: D005466:Fluorocarbons; D012702:Serotonin Antagonists; D005461:Fluorine; D012701:Serotonin; D006916:5-Hydroxytryptophan