RNAi expression vectors in plant cells.
Suppression by double-stranded RNA (dsRNA) of expression of a target gene is known as RNA interference (RNAi). Tobacco BY-2 cell suspension has been used as a model cultured plant cell, because it is possible to produce populations of tobacco BY-2 cell suspensions that are uniform and divide synchronously for functional gene analysis. Here, we describe a method to induce RNAi by introducing a hairpin-type dsRNA expression vector into BY-2 cells via electroporation. This methodology should facilitate the analysis of individual gene function in plant cells.
Akashi
Hideo
H
Gene Function Research Center, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan.
Miyagishi
Makoto
M
Taira
Kazunari
K
D000818:Animals; D001483:Base Sequence; D017930:Genes, Reporter; D005822:Genetic Vectors; D007202:Indicators and Reagents; D008156:Luciferases / Q000032:analysis / Q000235:genetics; D009690:Nucleic Acid Conformation; D010944:Plants / Q000235:genetics*; D012330:RNA, Double-Stranded / Q000037:antagonists & inhibitors / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics* / Q000494:pharmacology; D015183:Restriction Mapping
D007202:Indicators and Reagents; D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; D008156:Luciferases