Clara cell secretory protein: determination of serum levels by an enzyme immunoassay and its importance as an indicator of bronchial asthma in children.
Clara cell secretory protein (CC16) is a 16kDa protein secreted by Clara cells in the lining fluid of bronchiolar and bronchial epithelium. CC16 presents several biologic properties, and has been shown to have immunomodulatory and anti-inflammatory activity. It may play a role in controlling inflammation in the airway. There is some evidence that the CC16 level is primarily lower in adult individuals with bronchial asthma, thus contributing to its pathophysiology. This study was designed to examine CC16 serum levels of children, healthy and with asthma. An enzyme solid phase immunoassay utilizing monoclonal antibody to CC16 was the analytical method to determine the protein concentration in blood sera. The method showed excellent linearity, high sensitivity (detection limit: <50 ng/l) and precision. It was found that asthmatic children appear significantly lower levels (P < 0.001) of CC16 in serum as compared to healthy ones. It is, therefore, concluded that CC16 may be a useful diagnostic index of bronchial asthma in the early child-age.
Gioldassi
X M
XM
Laboratory of Biochemistry, Department of Chemistry, University of Patras, 26 500 Patras, Greece.
Papadimitriou
H
H
Mikraki
V
V
Karamanos
N K
NK
D000911:Antibodies, Monoclonal / Q000276:immunology; D001249:Asthma / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006115:Greece; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D007223:Infant; D014598:Uteroglobin / Q000097:blood* / Q000737:chemistry
D000911:Antibodies, Monoclonal; C556389:SCGB1A1 protein, human; D014598:Uteroglobin