Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection.
We report for the first time the application of a biochip using the molecular beacon (MB) detection scheme. The usability of this biochip novel detection system for the analysis of the breast cancer gene BRCA1 is demonstrated using molecular beacon probes. The MB is designed for the BRCA1 gene and a miniature biochip system is used for detection. The performance of the biochip-MB detection system is evaluated. The optimum conditions for the MB system for highest fluorescence detection sensitivity are investigated for the detection system. The detection of BRCA1 gene is successfully demonstrated in solution and the limit of detection (LOD) is estimated as 70 nM.
Culha
Mustafa
M
Advanced Biomedical Science and Technology Group, Oak Ridge National Laboratory, Life Science Division, Bethel Valley Road, MS-6101, P.O. Box 2008, Oak Ridge, TN 37831-6101, USA.
Stokes
David L
DL
Griffin
Guy D
GD
Vo-Dinh
Tuan
T
D015374:Biosensing Techniques; D015342:DNA Probes; D005453:Fluorescence; D019398:Genes, BRCA1
D015342:DNA Probes