title: [Study on extraction process of zhanjin ruji].
abstract: OBJECTIVE
  To select the optimum extraction process of Zhanjin Ruji.
  
  
  METHODS
  To observe influence of extraction time upon the extraction rate of volatile oil, the orthogonal test was adopted to observe the extraction process by alcohol from the extraction rate and content of the total saponins in Radix Notoginseng.
  
  
  RESULTS
  The three kinds of herbs including Radix Angelicae Sinensis, Resina Olibani and Myrrha were extracted with water for 3 hours, 95% of volatile oil can be distilled. The three kinds of herbs including Radix Notoginseng, Herba Lycopodii and Radix Gentianae Macrophyllac were extracted by alcohol. Four factors such as alcohol concentration(A), extraction times(B), extraction time(C), and solvent amount(D), had not significant effect on the content of total saponins in Radix Notoginseng in herbal extraction, but factor A and B had significant effect on the extraction rate. The optimum extraction process was as follows extracted with 5 times the amount of the solvent volum 60% alcohol for 3 times and with each time for 1 hour. Three times experiments showed that the extraction rate was 26.5% and the content of the total saponins in Radix Notoginseng was 17.28% mg.g-1.
  
  
  CONCLUSIONS
  The above experimental results can provide experimental basis for deciding the extraction process of Zhanjin Ruji.
authors.affiliation: Henan Provencial Institute of Luoyang Orthopedics, Luoyang 471002, Henan, China.
authors.forename: Zhi-qian
authors.initials: ZQ
authors.lastname: Du
authors.forename: Tian-xin
authors.initials: TX
authors.lastname: Du
authors.forename: Zhong-dong
authors.initials: ZD
authors.lastname: Wang
authors.forename: Gen-lin
authors.initials: GL
authors.lastname: Li
mesh_terms: D029971:Angelica sinensis / Q000737:chemistry; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000302:isolation & purification; D030012:Gentiana / Q000737:chemistry; D032502:Lycopodium / Q000737:chemistry; D008419:Materia Medica / Q000008:administration & dosage; D009822:Oils, Volatile / Q000302:isolation & purification; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012503:Saponins / Q000302:isolation & purification; D013678:Technology, Pharmaceutical / Q000379:methods*; D013729:Terpenes / Q000302:isolation & purification
chemical_list: D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D008419:Materia Medica; D009822:Oils, Volatile; D012503:Saponins; D013729:Terpenes; C014119:myrrh oil