[Immunodetection of RNA-dependent RNA polymerase from turnip yellow luteovirus using monoclonal antibodies].
Monoclonal antibodies (MAs) to the RNA-dependent RNA polymerase from turnip yellow luteovirus (TYV) were prepared using a recombinant protein as immunogen and were shown to be directed to C-terminal part of the viral replicase. These MAs were found to interact with a 70-kDa protein found in extracts from TYV-infected plants. Our result is the first successful attempt at detecting the RNA-dependent RNA polymerase of a luteovirus in infected plant extracts. We also found that the protein is not processed further and its accumulation and content in the infected plant obey a definite dynamics during the infection. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 1; see also http://www.maik.ru.
Sukhacheva
E A
EA
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP Moscow, 117997 Russia. eas@mail.ibch.ru
Fomicheva
V V
VV
Efimova
N A
NA
Shubert
J
J
D000911:Antibodies, Monoclonal / Q000276:immunology*; D001483:Base Sequence; D017931:DNA Primers; D012324:RNA-Dependent RNA Polymerase / Q000032:analysis* / Q000276:immunology; D017806:Tymovirus / Q000201:enzymology*
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D012324:RNA-Dependent RNA Polymerase