Peripheral macrophage depletion reduces central nervous system parasitism and damage in Trypanosoma cruzi-infected suckling rats.
We aim at investigating the role of blood born macrophages on the brain reaction to Trypanosoma cruzi infection in suckling rats. This infection provoked the appearance of numerous ED1(+) cells in the neural parenchyma and increased the amount of meningeal and perivascular ED2(+) macrophages. CD8(+) and NKR(+) cells also occurred. Parenchymal blood vessels showed strong ICAM-1 and decreased occludin immunoreactivities. Selective depletion of peripheral macrophages by clodronate liposomes decreased tissue parasitism, nodular lesions, ICAM-1 upregulation and leukocyte infiltration. Occludin immunoreactivity remained as in uninfected animals. Our results indicate a role for blood-born macrophages in both parasite invasion and brain reaction. Microglia activation cannot be discarded.
Silva
Grace C
GC
Department of Morphology, Federal University of Minas Gerais, Av. Antonio Carlos, 6627, 31270.901 Belo Horizonte, Minas Gerais, Brazil.
Nagib
Patrícia R A
PR
Chiari
Egler
E
van Rooijen
Nico
N
Machado
Conceição R S
CR
Camargos
Elizabeth R S
ER
D018712:Analgesics, Non-Narcotic / Q000627:therapeutic use; D000818:Animals; D000833:Animals, Suckling / Q000382:microbiology; D002452:Cell Count; D002490:Central Nervous System / Q000098:blood supply / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D020807:Central Nervous System Parasitic Infections / Q000473:pathology*; D014355:Chagas Disease / Q000188:drug therapy* / Q000378:metabolism; D004002:Clodronic Acid / Q000627:therapeutic use*; D053331:Ectodysplasins; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007114:Immunization / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007371:Interferon-gamma / Q000097:blood; D007962:Leukocytes / Q000145:classification / Q000378:metabolism; D008081:Liposomes / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D009026:Mortality; D062793:Occludin; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014349:Trypanosoma cruzi; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D018712:Analgesics, Non-Narcotic; D053331:Ectodysplasins; C506019:Eda protein, mouse; D008081:Liposomes; D008565:Membrane Proteins; D062793:Occludin; C568986:Ocln protein, mouse; C568987:Ocln protein, rat; D014409:Tumor Necrosis Factor-alpha; D004002:Clodronic Acid; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma