title: Binding of Vibrio anguillarum to neutral glycosphingolipids from intestinal mucosa of rainbow trout (Oncorhynchus mykiss).
abstract: To test whether glycosphingolipids (GSLs) on the intestinal mucosa of rainbow trout (Oncorhynchus mykiss) serve as a binding receptor for Vibrio anguillarum, we analyzed neutral GSLs from rainbow trout intestinal mucosa and investigated the binding of bacteria to neutral GSLs. Two kinds of neutral GSLs, designated N-1 and N-2, were identified on high-performance thin-layer chromatography (TLC) plates. In TLC immunostaining tests, V. anguillarum bound only to galactosylceramide (GalCer), lactosylceramide and N-1 having the same TLC mobility as GalCer, but neither to glucosylceramide nor to N-2. These results suggest that N-1 is GalCer (Gal beta 1-1Cer) and also that N-1 (GalCer) on rainbow trout intestinal mucosa act as a receptor for V. anguillarum.
authors.affiliation: Laboratory of Veterinary Immunology, Division of Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Irie
authors.forename: Shinobu
authors.initials: S
authors.lastname: Watarai
authors.forename: Tadashi
authors.initials: T
authors.lastname: Iwasaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kodama
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005393:Fish Diseases / Q000382:microbiology*; D005699:Galactosylceramides / Q000378:metabolism; D006028:Glycosphingolipids / Q000378:metabolism*; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism*; D007790:Lactosylceramides / Q000378:metabolism; D017686:Oncorhynchus mykiss; D014733:Vibrio / Q000378:metabolism*; D014735:Vibrio Infections / Q000382:microbiology / Q000662:veterinary*
chemical_list: D005699:Galactosylceramides; D006028:Glycosphingolipids; D007790:Lactosylceramides