[The effect of Redix Scutellariae on butyrate of Porphyromonas endodontalis in vitro].
OBJECTIVE
To study the effect of Radix Scutellariae on the growth, metabolism of Porphyromonas endodontalis (P.e), as a preparation for studying the mechanism of Radix Scutellariae in treating pulp and periapical diseases.


METHODS
P.e was chosen as the experimental bacteria. Radix Scutellariae was extracted by means of reflux with 80% ethanol. The value of MIC of Radix Scutellariae was measured by minute amount serial dilusion test, and the production of butyrate was measured by high liquid chromatograph(HPLC).


RESULTS
Radix Scutellariae could inhibit the growth of P.e, of which the MIC was 100 mg/L. Following the increase in concentration of Radix Scutellariae, the amount of butyrate decreased to (3.527 +/- 0.009) mg/L, (3.048 +/- 0.005) mg/L, (2.490 +/- 0.011) mg/L, (2.209 +/- 0.016) mg/L, respectively (P < 0.05).


CONCLUSIONS
Radix Scutellariae could inhibit the growth and metabolism of P.e and might be an effective agent in treating pulp and periapical diseases.
Li
Ji-yao
JY
Key Lab. of Oral Biomedical Engineering, Ministry of Education, Sichuan University, Chengdu 610041, China.
Tang
Ya-ling
YL
Tan
Hong
H
Zhou
Xue-dong
XD
Zhang
Ping
P
D016866:Bacteroidaceae Infections / Q000382:microbiology; D002087:Butyrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003782:Dental Pulp / Q000382:microbiology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D045202:Porphyromonas endodontalis / Q000378:metabolism*; D031363:Scutellaria / Q000737:chemistry*
D002087:Butyrates; D004365:Drugs, Chinese Herbal