title: Effects of probiotic Lactobacillus acidophilus strain DSM13241 in healthy adult dogs.
abstract: OBJECTIVE
  To evaluate viability of a probiotic strain of Lactobacillus acidophilus in a dry dog food, determine its ability to survive transit through the gastrointestinal tract and populate the colon, and assess its effects on intestinal and systemic parameters.
  
  
  METHODS
  15 adult dogs.
  
  
  METHODS
  Dogs were sequentially fed a dry control food for 2 weeks, the same food supplemented with > 10(9) L. acidophilus for 4 weeks, and the control food again for 2 weeks. Fecal score was assessed daily, and fecal and blood samples were collected for enumeration of bacterial populations and measurement of hematologic variables.
  
  
  RESULTS
  Recovery of L. acidophilus from the supplemented food was 71% and 63% at the start and end of the study, respectively, indicating that the bacteria were able to survive manufacture and storage. The probiotic bacterium was detected in feces via ribotyping and RNA gene sequencing during the probiotic administration phase but not 2 weeks after cessation of administration. Administration of the probiotic-supplemented food was associated with increased numbers of fecal lactobacilli and decreased numbers of clostridial organisms. There were significant increases in RBCs, Hct, hemoglobin concentration, neutrophils, monocytes, and serum immunoglobin G concentration and reductions in RBC fragility and serum NO concentration.
  
  
  CONCLUSIONS
  These data indicate that L. acidophilus can be successfully incorporated into a dry dog food, survive transit through the canine gastrointestinal tract, and populate the colon and are associated with local and systemic changes. This probiotic bacterium may have the potential to enhance intestinal health and improve immune function in dogs.
authors.affiliation: Waltham Centre for Pet Nutrition, Waltham-on-the-Wolds, Melton Mowbray, Leicestershire, LE14 4RT, UK.
authors.forename: Marie-Louise A
authors.initials: ML
authors.lastname: Baillon
authors.forename: Zoe V
authors.initials: ZV
authors.lastname: Marshall-Jones
authors.forename: Richard F
authors.initials: RF
authors.lastname: Butterwick
mesh_terms: D000704:Analysis of Variance; D000821:Animal Feed / Q000382:microbiology*; D000818:Animals; D001772:Blood Cell Count; D004285:Dogs / Q000382:microbiology* / Q000502:physiology*; D005243:Feces / Q000382:microbiology; D007074:Immunoglobulin G / Q000097:blood; D017404:In Situ Hybridization, Fluorescence; D007779:Lactobacillus acidophilus; D008137:Longitudinal Studies; D015345:Oligonucleotide Probes; D019936:Probiotics
chemical_list: D007074:Immunoglobulin G; D015345:Oligonucleotide Probes