Stabilization of oligonucleotide-polyethylenimine complexes by freeze-drying: physicochemical and biological characterization.
In the present study the lyophilization of oligodeoxynucleotide-polyethylenimine (ODN-PEI) complexes was investigated regarding the maintenance of physicochemical properties and influence on biological activity. To achieve this, we used PEI of different molecular weights, in the range of 800-0.8 kDa, as complexing agents for unmodified ODN and ribozymes. The hydrodynamic diameter was measured by photon correlation spectroscopy (PCS) and the zeta potential was determined using laser Doppler anemometry (LDA) of ODN complexes with PEI derivatives of different molecular weights both before and after lyophilization. Atomic force microscopy (AFM) was used to visualize freshly prepared, stored and lyophilized complexes in solution. The biological activity of the ODN, as well as of plasmid DNA, in lyophilized PEI complexes was examined and compared to freshly prepared complexes using standard transfection assays. All PEI derivatives formed very small complexes with ODN displaying hydrodynamic diameters ranging from 15 to 30 nm. Marginal changes in size after lyophilization were observed for ODN-PEI complexes. In contrast, plasmid complexed with PEI was found to aggregate. In either cases minimal or no influence of the added amount of lyoprotectant was observed. The shape of the very small and highly condensed ODN complexes was not altered by lyophilization as seen in the AFM images. The transfection efficiency of lyophilized ribozyme-PEI complexes relative to freshly prepared complexes was approximately 100%, whereas a decrease was seen for lyophilized plasmid-PEI complexes. An additive of the lyoprotectants trehalose, mannitol or sucrose preserved biological activity. This study demonstrates the particular suitability of ODN-PEI complexes to be formulated as lyophilized systems with no loss in physical stability or biological activity.
Brus
Carola
C
Department of Pharmaceutics and Biopharmacy, Philipps-University Marburg, Ketzerbach 63, 35037 Marburg, Germany.
Kleemann
Elke
E
Aigner
Achim
A
Czubayko
Frank
F
Kissel
Thomas
T
D016475:3T3 Cells; D000818:Animals; D002241:Carbohydrates / Q000737:chemistry; D045744:Cell Line, Tumor; D055598:Chemical Phenomena; D002626:Chemistry, Pharmaceutical; D002627:Chemistry, Physical; D004247:DNA / Q000008:administration & dosage / Q000235:genetics; D005079:Excipients; D005260:Female; D005612:Freeze Drying; D018014:Gene Transfer Techniques; D017078:Laser-Doppler Flowmetry; D008156:Luciferases / Q000235:genetics; D051379:Mice; D018625:Microscopy, Atomic Force; D008970:Molecular Weight; D009841:Oligonucleotides / Q000008:administration & dosage* / Q000737:chemistry*; D010051:Ovarian Neoplasms / Q000235:genetics; D010316:Particle Size; D010957:Plasmids / Q000235:genetics; D011094:Polyethyleneimine / Q000737:chemistry*; D016337:RNA, Catalytic / Q000235:genetics; D014162:Transfection
D002241:Carbohydrates; D005079:Excipients; D009841:Oligonucleotides; D016337:RNA, Catalytic; D011094:Polyethyleneimine; D004247:DNA; D008156:Luciferases