Enriched gonadal migration of donor-derived gonadal primordial germ cells by immunomagnetic cell sorting in birds.
This study was conducted to evaluate whether immunomagnetic treatment could improve the retrieval and migration capacity of avian gonadal primordial germ cells (gPGCs) collected from gonads in 5.5-day-old chick and 5-day-old quail embryos, respectively. Collected gPGCs were loaded into a magnetic-activated cell sorter (MACS) after being conjugated with specific gPGC antibodies and either MACS-treated or non-treated cells in each species were subsequently transferred to the recipient embryos. MACS treatment significantly (P < 0.05) increased the population ratio of gPGCs in gonadal cells retrieved (0.74 to 33.4% in the chicken and 2.68 to 45.1% in the quail). This was due to decreased number of non-gPGCs in total cell population. MACS treatment further enhanced gonadal migration of gPGCs transferred in both species (10% vs. 80-85% in the chicken and 10-15% vs. 70-80% in the quail). Increase in the number of microinjected cells up to 600 cells/embryo did not eliminate such promoting effect. In conclusion, MACS treatment greatly increased the population ratio of avian gPGCs in gonadal cells, resulting improved gonadal migration in recipient embryos.
Kim
Jin Nam
JN
School of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
Kim
Mi A
MA
Park
Tae Sub
TS
Kim
Duk Kyung
DK
Park
Hyun Jeong
HJ
Ono
Tamao
T
Lim
Jeong Mook
JM
Han
Jae Yong
JY
D000818:Animals; D001717:Birds; D002452:Cell Count; D002465:Cell Movement; D002645:Chickens; D005854:Germ Cells / Q000166:cytology* / Q000276:immunology; D006066:Gonads / Q000166:cytology* / Q000276:immunology; D018189:Immunomagnetic Separation / Q000295:instrumentation / Q000379:methods*; D011784:Quail