Delivery agents for oligonucleotides.
This chapter provides a basic overview of most of the oligonucleotide delivery systems available for an in vitro use. Two major classes are described: systems that act through an endocytosis process (e.g., lipid-based vectors, nanoparticles, and polycations) and systems that by-pass this endocytosis process (e.g., peptides and pore-forming agents). Each technique is briefly described to allow a critical choice of the best delivery systems suitable for specific purposes in cultured cells.
Seksek
Olivier
O
Laboratoire de Physicochimie Biomoléculaire et Cellulaire, Université Pierre & Marie Curie, Paris, France.
Bolard
Jacques
J
D000818:Animals; D002478:Cells, Cultured; D004337:Drug Carriers; D004705:Endocytosis; D007202:Indicators and Reagents; D008081:Liposomes; D008322:Mammals; D008845:Microinjections / Q000379:methods; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides / Q000493:pharmacokinetics*; D010455:Peptides; D011107:Polylysine
D004337:Drug Carriers; D007202:Indicators and Reagents; D008081:Liposomes; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D010455:Peptides; D011107:Polylysine