title: Number of Edb motor units estimated using an adapted multiple point stimulation method: normal values and longitudinal studies in ALS and peripheral neuropathies.
abstract: OBJECTIVE
  To validate the adapted multiple point stimulation (AMPS) method to estimate the number of motor units (MUNE) from the extensor digitorum brevis (Edb) muscle.
  
  
  METHODS
  Twenty controls (10 young and 10 old) were examined on both sides and 10 patients with amyotrophic lateral sclerosis (ALS) and 5 with acute peripheral neuropathy (PN) were examined longitudinally on one side during a series of repeated electrophysiological sessions.
  
  
  RESULTS
  In the controls, the median MUNE and size of the motor unit action potentials (S-MUAPs) were found to be age-related (411 and 70 microVms in the young group; 164 and 142 microVms in the old group; P < 0.01), with a coefficient of variation of MUNE values of 27% and 20%, respectively. In the ALS group, the median MUNE value at diagnosis was 31 (P < 0.05 vs. controls), and during a mean follow-up period of 11.5 months a continuous decrease in the MUNE value was seen, together with an initial increase, followed by a later decrease in 4 cases, in S-MUAP size. In the PN group, the MUNE value was initially similar to that in controls, but then decreased, accompanied by an increase in S-MUAP size, and then showed a progressive increase, together with a decrease in S-MUAP size.
  
  
  CONCLUSIONS
  AMPS, a MUNE method developed in the upper extremity, also appears to be a useful procedure for quantifying changes in the MUNE value in the Edb muscle without specific software in order to study age-related changes or changes in patients with ALS or PN.
authors.affiliation: Service de Neurologie, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
authors.forename: Eric
authors.initials: E
authors.lastname: Albrecht
authors.forename: Thierry
authors.initials: T
authors.lastname: Kuntzer
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D000690:Amyotrophic Lateral Sclerosis / Q000503:physiopathology*; D016022:Case-Control Studies; D002452:Cell Count; D004558:Electric Stimulation / Q000379:methods; D004576:Electromyography / Q000379:methods*; D005260:Female; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D009046:Motor Neurons; D018482:Muscle, Skeletal / Q000294:innervation* / Q000503:physiopathology; D009469:Neuromuscular Junction / Q000503:physiopathology*; D010523:Peripheral Nervous System Diseases / Q000503:physiopathology*; D014034:Toes