[Relationship between neurotoxicity of 6-hydroxydopamine and glutamate transport].
OBJECTIVE
To study the relationship of neurotoxicity of 6-hydroxydopamine (6-OHDA) and the function of glutamate transporter.


METHODS
Using in vivo microdialysis together with high performance liquid chromatography (HPLC) to detect the alteration of glutamate in the striatum and extracellular fluid of the PC12 cell. The rate of apoptosis and the activity of PC12 cells are read in a flow cytometer and a photometer for enzyme-labeled assays. The function of glutamate transporter is decided by detecting the ability of L-[3H]-glutamate uptake.


RESULTS
6-OHDA was shown to induce apoptosis and decrease the activity of PC12 cells. Increased release of glutamate was also found in PC12 cells and the injured striatum of the PD rats. But glutamate uptake in PC12 cells and rat striatum synaptosomes are inhibited obviously.


CONCLUSIONS
The neurotoxicity of 6-hydroxydopamine is associated with declined function of glutamate transporters, which may be one important pathogenesis mechanisms of Parkinson's disease.
Meng
Chang-hong
CH
Department of Pharmacology and Neurobiology, Nanjing Medical University, Nanjing 210029, China.
Ding
Jian-hua
JH
He
Hai-rong
HR
Yang
Yan-ling
YL
Gu
Bing
B
Hu
Gang
G
D027322:Amino Acid Transport System X-AG / Q000187:drug effects*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D003342:Corpus Striatum / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D008297:Male; D016627:Oxidopamine / Q000633:toxicity*; D016716:PC12 Cells; D010300:Parkinson Disease / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D027322:Amino Acid Transport System X-AG; D018698:Glutamic Acid; D016627:Oxidopamine