Adherence of Streptococcus mutans to various restorative materials in a continuous flow system.
A continuous flow system was developed to evaluate the adhesion of Streptococcus mutans ATCC 25175 to filling materials (Ariston, Tetric, Dyract, Compoglass, Vitremer, Aqua Ionofil, Ketac Fil, amalgam, Galloy and ceramics as controls). Streptococcus mutans was added to saliva-coated test specimens, and a nutrient broth permanently supplied over a time period of 48 h and then the weight of plaque, the number and viability of the bacteria adhering to the materials were determined. The weights of artificial plaque on all filling materials tested were higher than those on ceramics, the highest values were measured on the glass-ionomers. The amount of plaque correlates with the surface roughness, whereas there was no correlation of the surface roughness with the number of colony-forming units (CFU) of S. mutans. The CFU of adhering S. mutans also depends on the viability of the bacteria. The plaque on Ketac Fil contained a high number of viable bacteria. The fluorides of glass-ionomers do not efficiently prevent the attachment and the viability of S. mutans.
Eick
S
S
Medical Microbiology, University Hospital of Jena, Jena, Germany. sigrun.eick@med.uni-jena.de
Glockmann
E
E
Brandl
B
B
Pfister
W
W
D001422:Bacterial Adhesion; D003188:Composite Resins; D003723:Dental Amalgam; D003773:Dental Plaque; D003793:Dental Restoration, Permanent / Q000379:methods; D005459:Fluorides; D005899:Glass Ionomer Cements; D012463:Saliva; D013295:Streptococcus mutans / Q000502:physiology*
D003188:Composite Resins; D005899:Glass Ionomer Cements; D003723:Dental Amalgam; D005459:Fluorides