[Protective effect of 3,4-oxo-isopropylidene-shikimic acid on vascular endothelial cell injured by hydrogen peroxide].
OBJECTIVE
To study the effect of 3,4-oxo-isopropylidene-shikimic acid (ISA) on H2O2 (200 mol.L-1, 4 h) injured human umbilical vein endothelial cells (HUVEC).


METHODS
Morphological change was observed under microscop. Cell viability was assessed by MTT assay. The release of intracellular lactate dehydrogenase (LDH) and NO was assessed by colorimetry. Radioimmunoassay was used to assess 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha).


RESULTS
Pretreatment with ISA for 6 h alleviated the morphological damage of H2O2 induced HUVECs. At the concentration of 1-100 mumol.L-1, ISA prevented the inhibitory effect on cell viability induced by H2O2 in dose-dependent manner, but increased the ratio of cell viability from 60.4% to 84.3%. ISA reduced LDH release and increased the level of NO and 6-keto-PGF1 alpha in H2O2 induced HUVECs.


CONCLUSIONS
ISA exerted protective effect on H2O2 injured HUVEC.
Ma
Yi
Y
Department of Pharmacology, Beijing University of Traditional Chinese Medicine, Beijing 100102, China.
Sun
Jian-ning
JN
Xu
Qiu-ping
QP
Guo
Ya-jian
YJ
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors; D007231:Infant, Newborn; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D012765:Shikimic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology
C479295:3,4-oxoisopropylideneshikimic acid; D020011:Protective Agents; D012765:Shikimic Acid; D009569:Nitric Oxide; D015121:6-Ketoprostaglandin F1 alpha; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase