Involvement of the change in chromatin structure in thymocyte apoptosis induced by phosphorylation of histones.
The inhibitors of protein phosphatase such as calyculin A and okadaic acid induced thymocyte apoptosis. DNA fragmentation was increased in the nuclei from thymocytes treated with calyculin A, which accelerated phosphorylation of histones. Judging from the circular dichroism analysis, the structure of soluble chromatin was changed by the treatment with calyculin A. These results suggest that the change in chromatin structure may be one of the molecular mechanisms of internucleosomal DNA fragmentation.
Enomoto
Riyo
R
Department of Pharmacology, Faculty of Pharmaceutical Sciences and High Technology Research Center, Kobe Gakuin University, Ikawadani-cho, Nishi-ku, Kobe 651-2180, Japan. enomoto@pharm.kobegakuin.ac.jp
Yoshida
Yukari
Y
Komai
Tomoe
T
Sugahara
Chiyoko
C
Yasuoka
Yuka
Y
Lee
Eibai
E
D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002843:Chromatin / Q000648:ultrastructure*; D053938:DNA Fragmentation; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008387:Marine Toxins; D008954:Models, Biological; D010080:Oxazoles / Q000494:pharmacology*; D010766:Phosphorylation; D013601:T-Lymphocytes / Q000502:physiology / Q000648:ultrastructure*
D002843:Chromatin; D004791:Enzyme Inhibitors; D006657:Histones; D008387:Marine Toxins; D010080:Oxazoles; C059041:calyculin A