Intestinal ischemia-reperfusion injury augments intestinal mucosal injury and bacterial translocation in jaundiced rats.
OBJECTIVE
The aim of this study was to evaluate local effects and degree of bacterial translocation related with intestinal ischemia-reperfusion injury in a rat obstructive jaundice model.


METHODS
Thirty adult Sprague-Dawley rats (200-250 g) were divided into three groups; including Group 1 (jaundice group), Group 2 (jaundice-ischemia group) and Group 3 (ischemia group). All rats had 2 laparotomies. After experimental interventions, tissue samples for translocation; liver and ileum samples for histopathological examination, 25 cm of small intestine for mucosal myeloperoxidase and malondialdehyde levels and blood samples for biochemical analysis were obtained.


RESULTS
Jaundiced rats had increased liver enzyme levels and total and direct bilirubin levels (p<0.05). Intestinal mucosal myeloperoxidase and malondialdehyde levels were found to be high in intestinal ischemia-reperfusion groups (p<0.05). Intestinal mucosal damage was more severe in rats with intestinal ischemia-reperfusion after bile duct ligation (p<0.05). Degree of bacterial translocation was also found to be significantly high in these rats (p<0.05).


CONCLUSIONS
Intestinal mucosa is disturbed more severely in obstructive jaundice with the development of ischemia and reperfusion. Development of intestinal ischemia-reperfusion in obstructive jaundice increases bacterial translocation.
Yüksek
Yunus Nadi
YN
Department of General Surgery, Ankara Numune Hospital, Ankara, Turkey.
Kologlu
Murat
M
Daglar
Gül
G
Doganay
Mutlu
M
Dolapci
Istar
I
Bilgihan
Ayse
A
Dolapçi
Mete
M
Kama
Nuri Aydin
NA
D000818:Animals; D018988:Bacterial Translocation; D004195:Disease Models, Animal; D007413:Intestinal Mucosa / Q000098:blood supply* / Q000473:pathology*; D041781:Jaundice, Obstructive / Q000382:microbiology*; D008315:Malondialdehyde / Q000032:analysis; D009195:Peroxidase / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000382:microbiology* / Q000473:pathology*
D008315:Malondialdehyde; D009195:Peroxidase