[Antioxidation activity and protective effection of ginger oil on DNA damage in vitro].
OBJECTIVE
To study the antioxidation activity and protective effect of ginger oil on DNA damage.


METHODS
Chemical light assay was used to detect the oxygen radicals scavenging capacity of ginger oil. The erythrocyte oxidation damage was induced by H2O2. The effect of ginger oil on oxidative erythrocyte was observed by the colorimetric analysis assay, and the content of malondialdehyde (MDA) in rabit hepatocyte was measured. The anaylsis of DNA damage was made with single cell gel electrophoresis(SCGE) technique.


RESULTS
Ginger oil might decrease light value compared with control group and inhibited erythrocyte oxidation damage. Compared with that in control group, the degress of DNA damage reduced significantly in the protected groups. Ginger oil might decrease the content of MDA remarkably and inhitibition rate was 48.16%.


CONCLUSIONS
Ginger oil has dominantive protective effect on DNA damage induced by H2O2. Ginger oil might act as a scavenger of oxygen radical and might be used as an antioxidant.
Lu
Ping
P
Beijing Military Medical College of PLA, Beijing 100071, China. Luping947693@Sina.com
Lai
Bing-sen
BS
Liang
Ping
P
Chen
Zhen-tong
ZT
Shun
Shu-qing
SQ
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects; D020939:Zingiber officinale / Q000737:chemistry; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008099:Liver / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D010938:Plant Oils / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D020011:Protective Agents / Q000494:pharmacology*; D011817:Rabbits; D017382:Reactive Oxygen Species / Q000378:metabolism
D000975:Antioxidants; D010938:Plant Oils; D020011:Protective Agents; D017382:Reactive Oxygen Species; D008315:Malondialdehyde