Induction of apoptosis by erucylphospho-N,N,N-trimethylammonium is associated with changes in signal molecule expressionand location.
At concentrations effecting apoptosis, the alkylphosphocholine ErPC3 induced increased expression of the Rb protein in breast cancer (MCF-7) and leukemia (SKW-3, AR-230) cell lines as well as hypophosphorylation (K-562, CMLT-1, DOHH-2) and fragmentation of Rb (BV-173, SKW-3) in leukemia cell lines. ErPC3 exerts at least part of its antineoplastic activity by apoptosis, and this chain of events comprises early changes in the lipid raft fraction of the cellular membrane as well as modulation of different signal molecules, such as Abl, Bcr-Abl (fusion protein), and Rb.
Berger
Martin R
MR
Unit of Toxicology and Chemotherapy, German Cancer Research Center, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany. M.Berger@Dkfz-Heidelberg.de
Tsoneva
Iana
I
Konstantinov
Spiro M
SM
Eibl
Hansjörg
H
D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D020014:K562 Cells; D007938:Leukemia; D010755:Organophosphates / Q000494:pharmacology*; D010766:Phosphorylation; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D016160:Retinoblastoma Protein / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
D010755:Organophosphates; D000644:Quaternary Ammonium Compounds; D016160:Retinoblastoma Protein; C472787:erucylphospho-N,N,N-trimethylpropylammonium