title: The autoantibody repertoire against copper- or macrophage-modified LDL differs in normolipidemics and hypercholesterolemic patients.
abstract: We have analyzed the antibody repertoire from normo- and hypercholesterolemic subjects to investigate how it can be related to macrophage-dependent modification of low-density lipoproteins, in comparison to the commonly used copper-oxidized LDL. Preexisting natural antibodies in plasma from normo- and hypercholesterolemic individuals were tested for their reactivity against copper ion oxidized LDL and LDL modified by macrophages. A crosswise comparison between these two antigen preparations demonstrated a different antibody repertoire in normo- and hypercholesterolemic patients. This study suggest that the search for antibodies that can influence the progression or regression of an atherosclerotic process has to take into account the process by which LDL is modified, and the repertoire of antibodies that is generated in the normal population, in comparison to that with, or at risk for, coronary artery diseases.
authors.affiliation: Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, Sao Paulo, Brazil.
authors.forename: Eva C
authors.initials: EC
authors.lastname: Fernvik
authors.forename: Daniel F J
authors.initials: DF
authors.lastname: Ketelhuth
authors.forename: Momtchilo
authors.initials: M
authors.lastname: Russo
authors.forename: Magnus
authors.initials: M
authors.lastname: Gidlund
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D016022:Case-Control Studies; D002852:Chromatography, Ion Exchange; D019327:Copper Sulfate / Q000737:chemistry; D003324:Coronary Artery Disease / Q000209:etiology / Q000276:immunology; D005260:Female; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood / Q000276:immunology*; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry / Q000276:immunology*; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D010084:Oxidation-Reduction
chemical_list: D001323:Autoantibodies; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D019327:Copper Sulfate