title: [Isolation of conjugates of albumin and ribonuclease].
abstract: A method for separation of albumin-ribonuclease (RNase) conjugates has been proposed, based on the use of macroporous silicates. It was established that about 76% of ligand-free human serum albumin (LFHSA) formed complexes with enzymes. It was shown that most of the conjugates of albumin and pancreatic RNase contained up to 2 mol enzyme per 1 mol LFHSA. The conjugates of albumin and bacterial RNase, isolated from the cells of the strain Bacillus intermedius 7P, displayed higher specific activities, containing, on average, 2.3 mol RNase per 1 mol LFHSA (for the conjugates with molecular weights below 92 kDa) or 3.3 mol RNase per 1 mol protein carrier (for the conjugates with higher molecular weight).
authors.affiliation: Pacific Institute of Bioorganic Chemistry, Russian Academy of Sciences, Vladivostok, 690022 Russia. zel@piboc.dvo.ru
authors.forename: E A
authors.initials: EA
authors.lastname: Zelepuga
authors.forename: N V
authors.initials: NV
authors.lastname: Katushkina
authors.forename: V M
authors.initials: VM
authors.lastname: Kolikov
mesh_terms: D008970:Molecular Weight; D012260:Ribonucleases / Q000737:chemistry / Q000378:metabolism*; D012709:Serum Albumin / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D012709:Serum Albumin; D012260:Ribonucleases