A streptavidin-biotin-based microarray platform for immunoassays.
The authors describe a microarray system for disease diagnosis based on antibody-antigen interactions. Either biotinylated antibodies or antigens are coupled via streptavidin linkers onto a gold surface. This platform has been used to establish recombinant antibody-antigen interactions and to detect specific IgM antibodies in sera of patients suspected of Lyme borreliosis. Therefore, this microarray system can be adapted for further applications.
Pavlickova
Petra
P
Section of Experimental Anesthesiology, University Clinic Ulm, Germany.
Hug
Hubert
H
D000818:Animals; D000941:Antigens / Q000378:metabolism; D001710:Biotin / Q000378:metabolism*; D006046:Gold / Q000378:metabolism; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007128:Immunoglobulin Fragments / Q000378:metabolism; D007075:Immunoglobulin M / Q000097:blood; D008193:Lyme Disease / Q000175:diagnosis / Q000276:immunology; D010455:Peptides / Q000378:metabolism; D040081:Protein Array Analysis / Q000379:methods*; D015203:Reproducibility of Results; D019809:Streptavidin / Q000378:metabolism*
D000941:Antigens; D007128:Immunoglobulin Fragments; D007075:Immunoglobulin M; D010455:Peptides; D001710:Biotin; D006046:Gold; D019809:Streptavidin