title: Antigenic secreted proteins from Haemophilus paragallinarum. A 110-kDa putative RTX protein.
abstract: Haemophilus paragallinarum is the causal agent of infectious coryza, an economically important disease for the poultry industry. This bacterium secreted proteins of 25-110 kDa during its growth in brain heart infusion, tryptic soy broth, or Luria-Bertani glucose phosphate media, all lacking serum. Some of these proteins were recognized by sera from chickens experimentally infected with H. paragallinarum. A 110-kDa protein was recognized by a serum pool from convalescent-phase pigs naturally infected with Actinobacillus pleuropneumoniae, and also by a rabbit polyclonal serum against Apx I as well as a rabbit serum against Mannheimia haemolytica leukotoxin, suggesting the presence of an RTX-like protein in H. paragallinarum. H. paragallinarum secreted proteins could be important immunogens in the control of infectious coryza.
authors.affiliation: Centro de Investigaciones en Ciencias Microbiológicas, BUAP, Apdo. Postal 1622, Puebla 72560, Mexico.
authors.forename: Erika
authors.initials: E
authors.lastname: Mena-Rojas
authors.forename: Candelario
authors.initials: C
authors.lastname: Vázquez Cruz
authors.forename: Sergio
authors.initials: S
authors.lastname: Vaca Pacheco
authors.forename: Octavio
authors.initials: O
authors.lastname: García González
authors.forename: Víctor M
authors.initials: VM
authors.lastname: Pérez-Márquez
authors.forename: Alma
authors.initials: A
authors.lastname: Pérez-Méndez
authors.forename: Jorge
authors.initials: J
authors.lastname: Ibarra-Caballero
authors.forename: Mireya
authors.initials: M
authors.lastname: de la Garza
authors.forename: Edgar
authors.initials: E
authors.lastname: Zenteno
authors.forename: Erasmo
authors.initials: E
authors.lastname: Negrete-Abascal
mesh_terms: D000189:Actinobacillus Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary; D016977:Actinobacillus pleuropneumoniae / Q000276:immunology; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology* / Q000378:metabolism; D001426:Bacterial Proteins / Q000276:immunology* / Q000378:metabolism; D015153:Blotting, Western; D002645:Chickens / Q000276:immunology; D003429:Cross Reactions; D003470:Culture Media; D005098:Exotoxins / Q000276:immunology; D006192:Haemophilus Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary; D044134:Haemophilus paragallinarum / Q000254:growth & development / Q000276:immunology* / Q000378:metabolism*; D016978:Mannheimia haemolytica / Q000276:immunology; D008970:Molecular Weight; D016871:Pasteurellaceae Infections / Q000276:immunology / Q000662:veterinary; D011201:Poultry Diseases / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D013552:Swine / Q000276:immunology; D013553:Swine Diseases / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003470:Culture Media; D005098:Exotoxins; C018579:leukotoxin