title: Antibody targeting of the CC chemokine ligand 5 results in diminished leukocyte infiltration into the central nervous system and reduced neurologic disease in a viral model of multiple sclerosis.
abstract: Intracerebral infection of mice with mouse hepatitis virus, a member of the Coronaviridae family, reproducibly results in an acute encephalomyelitis that progresses to a chronic demyelinating disease. The ensuing neuropathology during the chronic stage of disease is primarily immune mediated and similar to that of the human demyelinating disease multiple sclerosis. Secretion of chemokines within the CNS signals the infiltration of leukocytes, which results in destruction of white matter and neurological impairment. The CC chemokine ligand (CCL)5 is localized in white matter tracts undergoing demyelination, suggesting that this chemokine participates in the pathogenesis of disease by attracting inflammatory cells into the CNS. In this study, we administer a mAb directed against CCL5 to mice with established mouse hepatitis virus-induced demyelination and impaired motor skills. Anti-CCL5 treatment decreased T cell accumulation within the CNS based, in part, on viral Ag specificity, indicating the ability to differentially target select populations of T cells. In addition, administration of anti-CCL5 improved neurological function and significantly (p < or = 0.005) reduced the severity of demyelination and macrophage accumulation within the CNS. These results demonstrate that the severity of CNS disease can be reduced through the use of a neutralizing mAb directed against CCL5 in a viral model of demyelination.
authors.affiliation: Center for Immunology and Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA.
authors.forename: William G
authors.initials: WG
authors.lastname: Glass
authors.forename: Michelle J
authors.initials: MJ
authors.lastname: Hickey
authors.forename: Jenny L
authors.initials: JL
authors.lastname: Hardison
authors.forename: Michael T
authors.initials: MT
authors.lastname: Liu
authors.forename: Jerry E
authors.initials: JE
authors.lastname: Manning
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Lane
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000627:therapeutic use; D002464:Cell Migration Inhibition; D002490:Central Nervous System / Q000276:immunology / Q000473:pathology* / Q000821:virology; D018946:Chemokine CCL5 / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D019742:Chemokines, CC / Q000276:immunology / Q000378:metabolism; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D018352:Coronavirus Infections / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D003711:Demyelinating Diseases / Q000276:immunology / Q000473:pathology / Q000517:prevention & control; D004195:Disease Models, Animal; D004679:Encephalomyelitis / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D007274:Injections, Intraperitoneal; D008024:Ligands; D008262:Macrophage Activation / Q000276:immunology; D008264:Macrophages / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009103:Multiple Sclerosis / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control; D006517:Murine hepatitis virus / Q000276:immunology; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D012720:Severity of Illness Index; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000473:pathology / Q000821:virology
chemical_list: D000911:Antibodies, Monoclonal; C483024:CCL5 protein, human; D018946:Chemokine CCL5; D019742:Chemokines, CC; D008024:Ligands; D012333:RNA, Messenger