title: Microglia enhance dorsal root ganglion outgrowth in Schwann cell cultures.
abstract: Transplantation of cellular populations to facilitate regrowth of damaged axons is a common experimental therapy for spinal cord injury. Schwann cells (SC) or microglia grafted into injury sites can promote axonal regrowth of central projections of dorsal root ganglion (DRG) sensory neurons. We sought to determine whether the addition of microglia or microglia-derived secretory products alters DRG axon regrowth upon cultures of SC. Rat DRG explants were grown on monolayers consisting of either SC, microglia, SC exposed to microglia-conditioned medium (MCM), or co-cultures with different relative concentrations of microglia. Image analysis revealed that, compared to SC alone, the extent of neurite outgrowth was significantly greater on SC-microglia co-cultures. Immunocytochemistry for extracellular matrix molecules showed that microglial cells stained positively for growth-promoting thrombospondin, whereas laminin and the inhibitory chondroitin sulfate proteoglycans (CSPGs) were localized primarily to SC. Notably, immunoreactivity for CSPGs appeared reduced in areas associated with DRG outgrowth in co-cultures and SC exposed to MCM. These results show that microglia or their secreted products can augment SC-mediated DRG regrowth in vitro, indicating that co-grafting SC with microglia provides a novel approach to augment sensory fiber regeneration after spinal cord injury.
authors.affiliation: Department of Anatomy and Neurobiology, University of Kentucky Medical Center, Lexington, Kentucky 40536, USA.
authors.forename: Dianna L
authors.initials: DL
authors.lastname: Hynds
authors.forename: Nagarathnamma
authors.initials: N
authors.lastname: Rangappa
authors.forename: Julia
authors.initials: J
authors.lastname: Ter Beest
authors.forename: Diane M
authors.initials: DM
authors.lastname: Snow
authors.forename: Alexander G
authors.initials: AG
authors.lastname: Rabchevsky
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000502:physiology*; D002455:Cell Division; D002478:Cells, Cultured; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005109:Extracellular Matrix; D005727:Ganglia, Spinal / Q000166:cytology*; D017628:Microglia / Q000166:cytology*; D009475:Neurons, Afferent / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology*
chemical_list: D017077:Culture Media, Conditioned