The interaction of native DNA with iron(III)- N ,N'-ethylene-bis(salicylideneiminato)-chloride.
The interaction between native calf thymus deoxyribonucleic acid (DNA) and Fe(III)- N ,N'-ethylene-bis (salicylideneiminato)-chloride, Fe(Salen)Cl, was investigated in aqueous solutions by UV-visible (UV-vis) absorption, circular dichroism (CD), thermal denaturation and viscosity measurements. The results obtained from CD, UV-vis and viscosity measurements exclude DNA intercalation and can be interpreted in terms of an electrostatic binding between the Fe(Salen)(+) cation and the phosphate groups of DNA. The trend of the UV-vis absorption band of the Fe(Salen)Cl complex at different ratios [DNA(phosphate)]/[Fe(Salen)Cl] and the large increase of the melting temperature of DNA in the presence of Fe(Salen)Cl, support the hypothesis of an external electrostatic interaction between the negatively charged DNA double helix and the axially stacked positively charged Fe(Salen)(+) moieties, analogously to what reported for a number of porphyrazines and metal-porphyrazine complexes interacting with DNA.
Silvestri
Arturo
A
Dipartimento di Chimica Inorganica e Analitica "S Cannizzaro", Università di Palermo, Viale delle Scienze, Parco d'Orleans II, I-90128, Palermo, Italy. asilves@unipa.it
Barone
Giampaolo
G
Ruisi
Giuseppe
G
Lo Giudice
Maria Teresa
MT
Tumminello
Salvatore
S
D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D007503:Iron Isotopes; D009691:Nucleic Acid Denaturation; D012545:Schiff Bases / Q000737:chemistry*; D013057:Spectrum Analysis; D014783:Viscosity
C484842:Fe(III)- N ,N'-ethylene-bis (salicylideneiminato)-chloride; D005290:Ferric Compounds; D007503:Iron Isotopes; D012545:Schiff Bases; D004247:DNA