Performance characteristics of updated INNO-LiPA assays for molecular typing of human leukocyte antigen A (HLA-A), HLA-B, and HLA-DQB1 alleles.
We carried out a multicenter performance evaluation of three new DNA-based human leukocyte antigen (HLA) typing assays: INNO-LiPA HLA-A Update, INNO-LiPA HLA-B Update, and INNO-LiPA HLA-DQB1 Update. After optimization, the accuracy rates were all 100%, and the final observed resolutions were 99.4, 92.4, and 85.6%, respectively. These rapid and easy-to-perform assays yielded results fully concordant with other DNA-based tissue typing tests.
De Vreese
Karen
K
Innogenetics N.V., B-9052 Ghent, Belgium. Karen_De_Vreese@innogenetics.be
Barylski
Rachel
R
Pughe
Fiona
F
Bläser
Miriam
M
Evans
Colin
C
Norton
John
J
Semana
Gilbert
G
Holman
Richard
R
Loiseau
Pascale
P
Masson
Dominique
D
Gielis
Mieke
M
De Brauwer
Annelies
A
De Canck
Ilse
I
Verpooten
Gonda
G
Hulstaert
Frank
F
D005787:Gene Frequency; D015234:HLA-A Antigens / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans
D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen