title: Human PinX1, a potent telomerase inhibitor, is not involved in human gastrointestinal tract carcinoma.
abstract: PinX1 was isolated as a Pin2/TRF1 binding protein that also binds to the telomerase catalytic subunit hTERT. The gene is a potent telomerase inhibitor and a putative tumor suppressor since it inhibits telomerase activity and affects tumorigenicity in nude mice. This study investigated aberrations of PinX1 gene in gastrointestinal tract carcinomas (GITCs). We examined mutations, mRNA expression and promoter methylation of PinX1 gene in 15 GITC cell lines, and 20 patients with primary GITC. We found a missense mutation at codon 254 (AGC/TGC) in a colon and an esophageal carcinoma cell line, and in cancerous and matching normal tissues of 2 patients with primary GITC (10%). It might be a benign polymorphism. No hyper-methylation was found in the promoter region and the treatment by 5-Aza-2'-deoxycytidine did not affect PinX1 mRNA expression level in any of the cell lines. It was concluded that the human PinX1 does not affect tumorigenesis of human GITC.
authors.affiliation: Department of Pathology, Iwate Medical University, School of Medicine, Morioka 020-8505, Japan. chihaya@iwate-med.ac.jp
authors.forename: Yuji
authors.initials: Y
authors.lastname: Akiyama
authors.forename: Chihaya
authors.initials: C
authors.lastname: Maesawa
authors.forename: Kei
authors.initials: K
authors.lastname: Wada
authors.forename: Kentaro
authors.initials: K
authors.lastname: Fujisawa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Itabashi
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Noda
authors.forename: Takehisa
authors.initials: T
authors.lastname: Honda
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Sato
authors.forename: Kaoru
authors.initials: K
authors.lastname: Ishida
authors.forename: Akinori
authors.initials: A
authors.lastname: Takagane
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Saito
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Masuda
mesh_terms: D001374:Azacitidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002277:Carcinoma / Q000175:diagnosis / Q000235:genetics* / Q000628:therapy; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D018899:CpG Islands; D019175:DNA Methylation; D000077209:Decitabine; D005770:Gastrointestinal Neoplasms / Q000175:diagnosis / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D020125:Mutation, Missense; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D017422:Sequence Analysis, DNA; D019098:Telomerase / Q000037:antagonists & inhibitors / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; C440808:PINX1 protein, human; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins; D000077209:Decitabine; D019098:Telomerase; D001374:Azacitidine