Occurrence of saxitoxins as a major toxin in the ovary of a marine puffer Arothron firmamentum.
Eleven male and 14 female specimens of a marine puffer Arothron firmamentum were collected from Oita and Iwate Prefectures, Japan. The toxicity assay using mouse showed that only ovary and skin of the female specimens were toxic, the toxicity scores being 5-740 as paralytic shellfish poison and <5-30 MU/g as tetrodotoxin (TTX), respectively. The toxin extracts from the both tissues were then treated with cartridge columns, and subjected to high performance liquid chromatography and liquid chromatography-mass spectral analyses. In the analyses, saxitoxin (STX) and decarbamoylSTX (dcSTX) were identified as the major toxins in the ovary, while the skin contained only TTX.
Nakashima
Kazuhito
K
Japan Frozen Foods Inspection Corporation, Fukuoka Branch, Fukuoka 812-0016, Japan.
Arakawa
Osamu
O
Taniyama
Shigeto
S
Nonaka
Mamoru
M
Takatani
Tomohiro
T
Yamamori
Kunio
K
Fuchi
Yuichi
Y
Noguchi
Tamao
T
D000818:Animals; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D005260:Female; D007564:Japan; D008297:Male; D051379:Mice; D010053:Ovary / Q000737:chemistry*; D012530:Saxitoxin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry / Q000633:toxicity; D012623:Seawater; D012867:Skin / Q000737:chemistry*; D023721:Tetraodontiformes / Q000378:metabolism*; D013779:Tetrodotoxin / Q000032:analysis* / Q000633:toxicity; D014018:Tissue Distribution; D023382:Toxicity Tests, Acute
D012530:Saxitoxin; D013779:Tetrodotoxin; C034903:decarbamylsaxitoxin