Acute no-effect dose for in ova exposure to C3F7 tagged 5-hydroxytryptophan, a novel probe for investigating neural development.
OBJECTIVE
To create a probe for investigating neural development in chicks using magnetic resonance, a C(3)F(7) tag was bonded to the indole ring of 5-hydroxytryptophan (5-HTP). Immunoassays indicate that a low dose (5 microg/egg) administered in ova results in 0.5-1.0 microg accumulating in a 250 microL midbrain.


METHODS
Given the potential for developmental neurotoxicity of a compound that replaces an endogenous neurotransmitter, we investigated the effects of acute exposure during development.


RESULTS
No qualitative differences were observed between treatment groups. Differences in mortality rates between treatment groups were not statistically significant [X(2)(obs)=2.30, X(2)(crit) (df=2)=5.99, P>.05].


CONCLUSIONS
The no-effect dose for in ova administration of C(3)F(7) tagged5-HTP is 5 microg/egg on Day 17.
Dingman
Sherry
S
Marist College, Poughkeepsie, NY, USA. Sherry.Dingman@marist.edu
Hurlburt
Lara
L
Branch
Craig
C
D006916:5-Hydroxytryptophan / Q000633:toxicity; D000818:Animals; D002645:Chickens; D005461:Fluorine; D005466:Fluorocarbons / Q000737:chemistry / Q000633:toxicity*; D008279:Magnetic Resonance Imaging; D009420:Nervous System / Q000187:drug effects / Q000196:embryology*; D010063:Ovum / Q000187:drug effects; D012701:Serotonin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000633:toxicity; D012702:Serotonin Antagonists / Q000633:toxicity
D005466:Fluorocarbons; D012702:Serotonin Antagonists; D005461:Fluorine; D012701:Serotonin; D006916:5-Hydroxytryptophan