title: Postoperative plasma metabolic consequences of an osseous substitute implantation: analysis by fourier transform infrared spectroscopy.
abstract: In this study, we have investigated the postoperative plasma consequences of coral implantation into femoral condyle of rabbits. Analyses were performed using Fourier transform infrared (FT-IR) spectroscopy, a sensitive and nondestructive method, to evaluate plasma modifications one day, one week, one month, and three months after surgery. This technique showed that both surgery and implantation induced important changes of lipidic compounds one day after surgery, with a body lipolysis. Major modifications appeared one week after surgery, with a decrease of protein and saccharide contents, and an increase of amino acids accompanied by a noticeable inflammatory process. These last changes were revealed by a hierarchical classification in the 1430-1295 and 1137-1092 cm(-1) spectral regions, leading to two well-separated groups, before and one week after surgery. After a delayed time of three months and despite a substantial recovery for biomolecules such as proteins and fatty acids, some alterations are always observed, such as for amino acids, triglycerides, and glycerol. Moreover, molecules implicated in the inflammatory process had not reached their initial level.
authors.affiliation: INSERM U577, Groupe de Chimie Bio-organique, Université Victor Segalen Bordeaux 2, Bât 4a, 146, rue Léo Saignat, 33076 Bordeaux cedex, France.
authors.forename: Chrystelle
authors.initials: C
authors.lastname: Lorin
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Melin
authors.forename: Jean-Patrick
authors.initials: JP
authors.lastname: Chenu
authors.forename: Annie
authors.initials: A
authors.lastname: Perromat
authors.forename: Gérard
authors.initials: G
authors.lastname: Déléris
mesh_terms: D000222:Adaptation, Physiological; D000596:Amino Acids / Q000097:blood; D000768:Anesthesia, General / Q000009:adverse effects; D000818:Animals; D037421:Anthozoa; D001672:Biocompatible Materials / Q000494:pharmacology* / Q000633:toxicity; D001798:Blood Proteins / Q000032:analysis; D018786:Bone Substitutes / Q000494:pharmacology* / Q000633:toxicity; D005227:Fatty Acids / Q000097:blood; D005269:Femur / Q000601:surgery*; D005990:Glycerol / Q000097:blood; D007249:Inflammation / Q000097:blood* / Q000139:chemically induced / Q000209:etiology; D008055:Lipids / Q000097:blood; D008066:Lipolysis; D011183:Postoperative Complications / Q000097:blood* / Q000139:chemically induced / Q000209:etiology; D019736:Prostheses and Implants / Q000009:adverse effects; D011817:Rabbits; D017550:Spectroscopy, Fourier Transform Infrared; D013312:Stress, Physiological / Q000150:complications; D014280:Triglycerides / Q000097:blood
chemical_list: D000596:Amino Acids; D001672:Biocompatible Materials; D001798:Blood Proteins; D018786:Bone Substitutes; D005227:Fatty Acids; D008055:Lipids; D014280:Triglycerides; D005990:Glycerol