title: Meiotic segregation of a t(7;8)(q11.21;cen) translocation in two carrier brothers.
abstract: OBJECTIVE
  To determine the meiotic segregation of a t(7;8)(q11.21;cen) translocation in two carrier brothers.
  
  
  METHODS
  Analysis of sperm nuclei by fluorescence in situ hybridization (FISH).
  
  
  METHODS
  Franche-Comté University Fertility Center in Besançon, France.
  
  
  METHODS
  Two oligospermic brothers with a (7;8) translocation.
  
  
  METHODS
  Dual FISH for chromosomes 7 and 8, and multicolor FISH for chromosomes X, Y, 15, and 18 were performed.
  
  
  METHODS
  Meiotic segregation differences between both brothers and controls.
  
  
  RESULTS
  In dual FISH 7-8, a total of 34527 (older brother) and 10023 (younger brother) spermatozoa were analyzed. The frequencies of alternate, adjacent 1, adjacent 2, and 3:1 segregations and diploidies were 56.7%, 25.1%, 11.1%, 7.06%, and 0.04%, respectively, in the older brother and 62.84%, 17.61%, 12.8%, 6.47%, and 0.28% in the younger. Also, the disomy rates of some chromosomes not implicated in the translocation would suggest a possible interchromosomal effect.
  
  
  CONCLUSIONS
  The similar segregation profiles for the same translocation, compared with those very divergent profiles of the other cases of studied translocations that were published in the literature, confirm that the risks of meiotic imbalances vary primarily according to the characteristics of the chromosomes involved in the rearrangement and the breakpoint position.
authors.affiliation: Laboratoire d'Histologie, Cytogénétique et Embryologie, Faculté de Médecine et des Sciences de la Sante Université Bretagne Occidentale, Brest, France. frederic.morel@chu-brest.fr
authors.forename: Frédéric
authors.initials: F
authors.lastname: Morel
authors.forename: Nathalie
authors.initials: N
authors.lastname: Douet-Guilbert
authors.forename: Christophe
authors.initials: C
authors.lastname: Roux
authors.forename: Christophe
authors.initials: C
authors.lastname: Tripogney
authors.forename: Marie-Josée
authors.initials: MJ
authors.lastname: Le Bris
authors.forename: Marc
authors.initials: M
authors.lastname: De Braekeleer
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Bresson
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D020090:Chromosome Segregation; D004171:Diploidy; D005787:Gene Frequency; D006579:Heterozygote; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008540:Meiosis / Q000235:genetics*; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology*; D014178:Translocation, Genetic