title: Neutralization of interleukin-10 significantly enhances gamma interferon expression in peripheral blood by stimulation with Johnin purified protein derivative and by infection with Mycobacterium avium subsp. paratuberculosis in experimentally infected cattle with paratuberculosis.
abstract: Monoclonal antibody neutralization of interleukin-10 (IL-10) increased Johnin purified protein derivative-induced whole-blood gamma interferon (IFN-gamma) secretion 23-fold and also increased IFN-gamma secretion ninefold following in vitro Mycobacterium avium subsp. paratuberculosis infection of peripheral blood mononuclear cells. These results demonstrate the suppressive effect of IL-10 on immune responses to M. avium subsp. paratuberculosis infection in cattle.
authors.affiliation: Paratuberculosis and Inflammatory Bowel Disease Research Team. Immune System Section, National Institute of Animal Health. Molecular Immunology Laboratory, National Institute of Agrobiological Sciences, 3-1-5 Kan-nondai, Tsukuba 305-0856, Japan.
authors.forename: Joram J
authors.initials: JJ
authors.lastname: Buza
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Hikono
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Mori
authors.forename: Reiko
authors.initials: R
authors.lastname: Nagata
authors.forename: Sachiyo
authors.initials: S
authors.lastname: Hirayama
authors.lastname: Aodon-geril
authors.forename: Abusaleh M
authors.initials: AM
authors.lastname: Bari
authors.forename: Yujing
authors.initials: Y
authors.lastname: Shu
authors.forename: Noriko M
authors.initials: NM
authors.lastname: Tsuji
authors.forename: Eiichi
authors.initials: E
authors.lastname: Momotani
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000382:microbiology; D007371:Interferon-gamma / Q000378:metabolism*; D016753:Interleukin-10 / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000382:microbiology; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology* / Q000472:pathogenicity; D009500:Neutralization Tests; D010283:Paratuberculosis / Q000276:immunology* / Q000382:microbiology; D014373:Tuberculin / Q000276:immunology*; D015854:Up-Regulation
chemical_list: D014373:Tuberculin; D016753:Interleukin-10; D007371:Interferon-gamma