Polycyclic aromatic hydrocarbon-DNA adducts determined by semiquantitative immunohistochemistry in human esophageal biopsies taken in 1985.
Esophageal endoscopic biopsy samples were obtained in 1985 in Linxian, China, a region with very high esophageal cancer incidence rates, and where ingested food is known to contain substantial amounts of polycyclic aromatic hydrocarbons (PAHs). In this study, the automated cellular imaging system (ACIS) was used for localization and semi-quantitation of PAH-DNA adducts. Fresh tissue sections were cut from archived paraffin blocks and incubated with an antiserum elicited against DNA modified with 7beta,8alpha-dihydroxy-9alpha,10alpha-epoxy-7,8,9,10-tetrahydro-benzo[a]pyrene (BPDE). Nuclear PAH-DNA adduct staining was observed in four out of five human samples incubated with the anti-BPDE-DNA. By visual inspection, nuclei in the basal layer of the esophageal epithelium had higher levels of PAH-DNA adducts compared to those found in the adjacent superficial squamous layer. Nuclear PAH-DNA staining was absent in serial sections incubated with either normal rabbit serum or BPDE-DNA-antiserum previously absorbed with the immunogen BPDE-DNA. Semi-quantitative evaluation by ACIS revealed that per nucleus values for PAH-DNA adducts in the basal layer of the esophageal epithelium were 5- to 40-fold higher than those in the adjacent superficial squamous layer (P < 0.0001), using a random effects model). This pilot study demonstrates the presence of PAH-DNA adducts in archived paraffin-embedded endoscopic esophageal biopsy samples that are close to 20 years old, and suggests that an appropriate set of archived samples could be used to prospectively correlate PAH-DNA adduct formation with risk of esophageal cancer development.
van Gijssel
Hilde E
HE
Laboratory of Cellular Carcinogenesis and Tumor Promotion, Center for Cancer Research, NIH, 37 Convent Dr., MSC-4255, Bethesda, MD 20892-4255, USA.
Schild
Laura J
LJ
Watt
Danielle L
DL
Roth
Mark J
MJ
Wang
Guo-Qing
GQ
Dawsey
Sanford M
SM
Albert
Paul S
PS
Qiao
You-Lin
YL
Taylor
Philip R
PR
Dong
Zhi-Wei
ZW
Poirier
Miriam C
MC
D044466:Asian People; D002273:Carcinogens / Q000378:metabolism*; D018736:DNA Adducts / Q000032:analysis*; D003952:Diagnostic Imaging; D004724:Endoscopy; D004938:Esophageal Neoplasms / Q000378:metabolism / Q000473:pathology; D016629:Esophagectomy; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D010865:Pilot Projects; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000378:metabolism*; D012189:Retrospective Studies; D012307:Risk Factors
D002273:Carcinogens; D018736:DNA Adducts; D011084:Polycyclic Aromatic Hydrocarbons