title: Effects of membrane attack complex of complement on apoptosis in experimental autoimmune encephalomyelitis.
abstract: Complement activation is involved in the initiation of inflammation and antibody-mediated demyelination in experimental autoimmune encephalomyelitis (EAE). We investigated the role of MAC in apoptosis in myelin-induced EAE in complement C5-deficient (C5-d) and C5-sufficient (C5-s) mice. The number of apoptotic cells assessed by TUNEL assay was significantly increased in C5-d mice during clinical recovery as compared with C5-s mice. Most of the apoptotic cells were lymphocytes, monocytes, and oligodendrocytes. DNA microarray was performed using total RNA extracted from spinal cords. Genes expressed higher in C5-s included members of the caspase (caspase 6, 7), TNF and TNFR families (CD27, FasL, lymphotoxin-beta R) and survivin. These results indicate that C5 and possibly MAC may be required for the limitation of inflammatory response within the central nervous system.
authors.affiliation: University of Maryland, School of Medicine, Baltimore, Maryland 21201, USA.
authors.forename: Teodora
authors.initials: T
authors.lastname: Niculescu
authors.forename: Susanna
authors.initials: S
authors.lastname: Weerth
authors.forename: Lucian
authors.initials: L
authors.lastname: Soane
authors.forename: Florin
authors.initials: F
authors.lastname: Niculescu
authors.forename: Violeta
authors.initials: V
authors.lastname: Rus
authors.forename: Cedric S
authors.initials: CS
authors.lastname: Raine
authors.forename: Moon L
authors.initials: ML
authors.lastname: Shin
authors.forename: Horea
authors.initials: H
authors.lastname: Rus
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D003182:Complement C5 / Q000172:deficiency; D015938:Complement Membrane Attack Complex / Q000235:genetics*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000235:genetics / Q000473:pathology*; D005260:Female; D020287:In Situ Nick-End Labeling; D051379:Mice; D018345:Mice, Knockout; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D013116:Spinal Cord
chemical_list: D003182:Complement C5; D015938:Complement Membrane Attack Complex; D012313:RNA