Acclimation to hypothermic incubation in developing chicken embryos (Gallus domesticus): II. Hematology and blood O2 transport.
Hypothermic incubation reduces the ability of the late-stage chicken embryo to mount endothermic heat production. This study investigates whether blood O(2) transport is similarly limited by cooler incubation temperatures. Two populations of chicken embryos were incubated at 38 degrees C and 35 degrees C. At Hamilton-Hamburger (HH) developmental stage 41-42, hypothermic incubation had no significant effect on hematocrit ( approximately 26%) or [Hb] (7 g%). However, in the final stages of incubation (stage 43-44), hypothermic incubation reduced hematocrit from approximately 31% at 38 degrees C to approximately 27.5% at 35 degrees C. Hypothermic incubation similarly caused a reduced [Hb] from 8.5 g% (38 degrees C) to 6.25 g% (35 degrees C), indicating a reduction in blood O(2)-carrying capacity in embryos. Incubation temperature had a strong effect on blood-O(2) affinity in late development (stage 43-44), with P(50) at 38 degrees C falling significantly from approximately 6 kPa in 38 degrees C embryos to approximately 4 kPa in 35 degrees C embryos. P(O(2)) values in chorioallantoic arterial blood at HH 41-42 were 4.3 kPa at pH 7.46 (38 degrees C) and 3.4 kPa at pH 7.39 (35 degrees C). In chorioallantoic venous blood these values were 9.1 kPa at pH 7.34 (38 degrees C) and 8.1 kPa at pH 7.42 (35 degrees C). With further development to HH 43-44, chorioallantoic arterial blood oxygenation fell to 2.4 kPa at pH 7.54 (38 degrees C) and 2.8 kPa at pH 7.52 (35 degrees C). Similarly, P(O(2)) in chorioallantoic venous blood fell slightly to 7.7 kPa at pH 7.42 (38 degrees C) and 7.4 kPa at pH 7.48 (35 degrees C). Collectively, these data reveal that beyond HH 41-42, 35 degrees C embryos experience retarded hematological development, and the findings that support the observed delayed metabolic response to acute temperature changes.
Black
Juli L
JL
Department of Biological Sciences, University of North Texas, Denton, TX 76203, USA.
Burggren
Warren W
WW
D019794:2,3-Diphosphoglycerate / Q000032:analysis; D000064:Acclimatization / Q000502:physiology*; D000255:Adenosine Triphosphate / Q000032:analysis; D000704:Analysis of Variance; D000818:Animals; D002642:Chick Embryo / Q000196:embryology*; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000097:blood*; D013053:Spectrophotometry; D013696:Temperature; D013997:Time Factors
D006454:Hemoglobins; D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate; D010100:Oxygen