Serum protein profile alterations in hemodialysis patients.
BACKGROUND
Serum protein profiling patterns can reflect the pathological state of a patient and therefore may be useful for clinical diagnostics. Here, we present results from a pilot study of proteomic expression patterns in hemodialysis patients designed to evaluate the range of serum proteomic alterations in this population.


METHODS
Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) was used to analyze serum obtained from patients on periodic hemodialysis treatment and healthy controls. Serum samples from patients and controls were first fractionated into six eluants on a strong anion exchange column, followed by application to four array chemistries representing cation exchange, anion exchange, metal affinity and hydrophobic surfaces. A total of 144 SELDI-TOF-MS spectra were obtained from each serum sample.


RESULTS
The overall profiles of the patient and control samples were consistent and reproducible. However, 30 well-defined protein differences were observed; 15 proteins were elevated and 15 were decreased in patients compared to controls. Serum from 1 patient exhibited novel protein peaks suggesting possible additional changes due to a secondary disease process.


CONCLUSIONS
SELDI-TOF-MS demonstrated consistent serum protein profile differences between patients and controls. Similarity in protein profiles among dialysis patients suggests that patient physiological responses to end-stage renal disease and/or dialysis therapy have a major effect on serum protein profiles.
Langlois
Richard G
RG
Lawrence Livermore National Laboratory, Livermore, CA 94550, USA.
Trebes
James E
JE
Dalmasso
Enrique A
EA
Ying
Yong
Y
Davies
Robert W
RW
Curzi
Mario P
MP
Colston
Bill W
BW
Turteltaub
Kenneth W
KW
Perkins
Julie
J
Chromy
Brett A
BA
Choi
Megan W
MW
Murphy
Gloria A
GA
Fitch
J Patrick
JP
McCutchen-Maloney
Sandra L
SL
D000328:Adult; D001798:Blood Proteins / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis; D051437:Renal Insufficiency / Q000097:blood* / Q000628:therapy*
D001798:Blood Proteins