[Expression of NO and NOS in nasal polyps].
OBJECTIVE
To investigate the expression of nitric oxide synthase(NOS) in nasal polyps tissue and also to study the function of nitric oxide in the pathology of nasal polyps.


METHODS
Investigating the expression of iNOS, eNOS with immunohistochemical staining, and the expression of iNOS mRNA with hybridization in situ. Analysing the production of nitric oxide in the nasal polyps through nitrate reductase technique.


RESULTS
In the polyps group, eNOS located in gland cells, epithelial and vascular endothelial cells, the staining of which was a little stronger than those of controls. The staining of iNOS was fairly strong in the epithelial cells, and it expressed mostly in the inflammatory cells inside the tissue.


CONCLUSIONS
eNOS may play a role in glandular secretion and vascular dilatation. The increase of eNOS might respond to the enhanced nasal secretion and edema of nasal mocusa. Large amount of NO produced by iNOS may deteriorate the inflammation process of nasal polyps.
Chen
Jianjun
J
Department of Otorhinolaryngology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.
Xiang
Jisheng
J
D000328:Adult; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000378:metabolism; D009298:Nasal Polyps / Q000209:etiology / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000235:genetics; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D012333:RNA, Messenger; D009569:Nitric Oxide; C496319:NOS2 protein, human; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III