Purification, crystallization and preliminary X-ray analysis of uridine phosphorylase from Salmonella typhimurium.
The structural udp gene encoding uridine phosphorylase (UPh) was cloned from the Salmonella typhimurium chromosome and overexpressed in Escherichia coli cells. S. typhimurium UPh (StUPh) was purified to apparent homogeneity and crystallized. The primary structure of StUPh has high homology to the UPh from E. coli, but the enzymes differ substantially in substrate specificity and sensitivity to the polarity of the medium. Single crystals of StUPh were grown using hanging-drop vapor diffusion with PEG 8000 as the precipitant. X-ray diffraction data were collected to 2.9 A resolution. Preliminary analysis of the diffraction data indicated that the crystal belonged to space group P6(1(5)), with unit-cell parameters a = 92.3, c = 267.5 A. The solvent content is 37.7% assuming the presence of one StUPh hexamer per asymmetric unit.
Dontsova
Mariya V
MV
Institute of Crystallography, Russian Academy of Sciences, Russia, and Institute of Protein Research, Russian Academy of Sciences, Russia.
Savochkina
Yulia A
YA
Gabdoulkhakov
Azat G
AG
Baidakov
Sergey N
SN
Lyashenko
Andrey V
AV
Zolotukhina
Maria
M
Errais Lopes
Liubov
L
Garber
Mariya B
MB
Morgunova
Ekaterina Yu
EY
Nikonov
Stanislav V
SV
Mironov
Alexandr S
AS
Ealick
Steven E
SE
Mikhailov
Al 'Bert M
A'
D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D012486:Salmonella typhimurium / Q000201:enzymology*; D014543:Uridine Phosphorylase / Q000737:chemistry*
D014543:Uridine Phosphorylase