Imaging of bioluminescent LNCaP-luc-M6 tumors: a new animal model for the study of metastatic human prostate cancer.
BACKGROUND
Animal experiments examining hormone-sensitive metastatic prostate cancer using the human LNCaP cell line have been limited to endpoint analyses. To permit longitudinal studies, we generated a luciferase-expressing cell line and used bioluminescent imaging (BLI) to non-invasively monitor the in vivo growth of primary LNCaP tumors and metastasis.


METHODS
LNCaP.FGC cells were transfected to constitutively express firefly luciferase. LNCaP-luc-M6 cells were tested for bioluminescent signal intensity and hormone responsiveness in vitro. The cells were implanted in subcutaneous and orthotopic sites in SCID-bg mice and imaged over time.


RESULTS
The LNCaP-luc-M6 cells formed subcutaneous and orthotopic tumors in SCID-bg mice, and nearly all tumor-bearing animals developed pulmonary metastases. Early detection and temporal growth of primary tumors and metastatic lesions was successfully monitored by BLI.


CONCLUSIONS
The LNCaP-luc-M6 cell line is a bioluminescent, hormone-sensitive prostate cancer cell line applicable for BLI studies to non-invasively monitor subcutaneous and orthotopic prostate tumor growth and metastasis in vivo.
Scatena
Caroline D
CD
Xenogen Corporation, Alameda, California 94501, USA.
Hepner
Mischa A
MA
Oei
Yoko A
YA
Dusich
Joan M
JM
Yu
Shang-Fan
SF
Purchio
Tony
T
Contag
Pamela R
PR
Jenkins
Darlene E
DE
D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis* / Q000235:genetics*; D008163:Luminescent Measurements; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009362:Neoplasm Metastasis / Q000503:physiopathology*; D010641:Phenotype; D010957:Plasmids; D011471:Prostatic Neoplasms / Q000473:pathology* / Q000662:veterinary; D014162:Transfection; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D008156:Luciferases