Base pairing properties of D- and L-cyclohexene nucleic acids (CeNA).
Cyclohexene nucleic acids (CeNA) with a D-like configuration form very stable self-complementary duplexes and stable duplexes with RNA. An increased duplex stability with Delta T(m)/mod of +1.2 degrees C is observed. The duplex with DNA is less stable. Excellent mismatch discrimination has been observed as well for the duplex with DNA as for the duplex with RNA. The results obtained with mixed CeNA sequences warrant antisense studies with CeNA. The CeNAs of opposite chirality constitute a self-pairing system on their own, resembling L-RNA sequences.
Gu
Ping
P
Laboratory for Medicinal Chemistry, Rega Institute for Medical Research, B-3000 Leuven, Belgium.
Schepers
Guy
G
Rozenski
Jef
J
Van Aerschot
Arthur
A
Herdewijn
Piet
P
D020137:Base Pair Mismatch; D020029:Base Pairing; D003510:Cyclohexanes / Q000737:chemistry*; D053138:Cyclohexenes; D004247:DNA / Q000737:chemistry*; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*
D003510:Cyclohexanes; D053138:Cyclohexenes; D009692:Nucleic Acid Heteroduplexes; D009841:Oligonucleotides; C052568:cyclohexene; D012313:RNA; D004247:DNA