Ionophoric activity of pluronic block copolymers.
Pluronic block copolymers (triblock copolymers of poly(ethylene oxide) and poly(propylene oxide)) exhibit a chemosensitizing effect on multidrug resistant cell lines. Changes in membrane permeability are hypothesized to be responsible because inhibition of drug transport mediated by both the multidrug-resistance-associated protein and the P-glycoprotein drug efflux system has been observed. To test this hypothesis, we now have studied the ion conductivity mediated by Pluronic L61. Besides a detergent-like action, the copolymer was able to form regular channels and to exhibit carrier activity. Long living ion channels were formed by polymer oligomerization. Aggregate equilibrium was shifted toward L61 monomers and dimers, which operated as mobile carriers. Copolymer-induced membrane permeability for potassium ions (1 M KCl) was less than 10(-8) cm s(-1), whereas the permeability for uncharged doxorubicin molecules (1 mM) was equal to 5 x 10(-4) cm s(-1). The results are consistent with reports about an increased doxorubicin accumulation in cells (Venne, Li, S., Mandeville, R., Kabanov, A., and Alakhov, V. Y. (1996) Cancer Res. 56, 3626-3629). However, the increased permeability contrasts with the polymer-mediated decrease of drug efflux from cells. Preferential polymer binding to membrane proteins may mask the unspecific effect of L61 observed on lipid bilayers.
Krylova
Oxana O
OO
Forschungsinstitut für Molekulare Pharmakologie, Campus Berlin-Buch, Robert-Roessle Strasse 10, 13125 Berlin, Germany.
Pohl
Peter
P
D000327:Adsorption; D001692:Biological Transport; D019281:Dimerization; D004317:Doxorubicin / Q000737:chemistry; D004553:Electric Conductivity; D004852:Epoxy Compounds / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D007473:Ion Channels / Q000737:chemistry; D007476:Ionophores / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry; D008956:Models, Chemical; D018408:Patch-Clamp Techniques; D010539:Permeability; D020442:Poloxamer / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*
D004852:Epoxy Compounds; D007473:Ion Channels; D007476:Ionophores; D008051:Lipid Bilayers; D020442:Poloxamer; D011092:Polyethylene Glycols; D004317:Doxorubicin; C009068:propylene oxide