title: Correlation of acute humoral response with brain virus burden and survival time in pig-tailed macaques infected with the neurovirulent simian immunodeficiency virus SIVsmmFGb.
abstract: Infection of pig-tailed macaques with the simian immunodeficiency virus (SIV) isolate SIVsmmFGb frequently results in SIV encephalitis (SIVE) in addition to immunodeficiency and acquired immune deficiency syndrome. We used in situ hybridization to quantitate the number of SIV-infected cells in brain parenchyma, choroid plexus, and meninges from 17 macaques that developed acquired immune deficiency syndrome after infection with SIVsmmFGb. SIV-infected cells and histopathological lesions of SIVE were identified in 15 of 17 animals (88.2%), including 12 of 12 rapid progressors (RP) and 3 of 5 slow progressors (SP). The parenchymal virus burden was much greater in RP macaques than in the three SP macaques with SIVE (median values of 24.3 versus 0.3 infected cells/mm(2), respectively; P < 0.05). Viral load differences between RP and SP with SIVE were less marked in choroid plexus (29.6 versus 12.8 infected cells/mm(2), respectively) and meninges (133.0 versus 34.2 infected cells/mm(2), respectively). A significant negative correlation was observed between the magnitude of the anti-SIV antibody titer at 1 month after inoculation and brain virus burden at necropsy (r = -0.614; P < 0.01). The close association between immune response and SIVE in this model should prove useful for identifying correlates of immune protection against primate lentiviral encephalitis.
authors.affiliation: Division of Microbiology, Yerkes National Primate Research Center, Emory University School of Medicine, Atlanta, GA, USA. shawn_oneil@hms.harvard.edu
authors.forename: Shawn P
authors.initials: SP
authors.lastname: O'Neil
authors.forename: Carolyn
authors.initials: C
authors.lastname: Suwyn
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Anderson
authors.forename: Genevieve
authors.initials: G
authors.lastname: Niedziela
authors.forename: Juliette
authors.initials: J
authors.lastname: Bradley
authors.forename: Francis J
authors.initials: FJ
authors.lastname: Novembre
authors.forename: James G
authors.initials: JG
authors.lastname: Herndon
authors.forename: Harold M
authors.initials: HM
authors.lastname: McClure
mesh_terms: D000818:Animals; D000917:Antibody Formation; D001921:Brain / Q000473:pathology* / Q000821:virology*; D018792:Encephalitis, Viral / Q000150:complications / Q000276:immunology*; D005260:Female; D017403:In Situ Hybridization; D008254:Macaca nemestrina; D008297:Male; D012189:Retrospective Studies; D016097:Simian Acquired Immunodeficiency Syndrome / Q000150:complications / Q000276:immunology* / Q000401:mortality; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D019562:Viral Load