[Detection of oprI gene of Pseudomonas aeruginosa by reverse dot-blot hybridization].
OBJECTIVE
To establish a rapid method for detecting Pseudomonas aeruginosa at the early stage of infection.


METHODS
Specific primers were designed according to oprI gene sequence of Pseudomonas aeruginosa, and the specific probe was synthesized by PCR. After photosensitive biotin labeling of the bacterial DNA, reverse dot-blot hybridization was used to detect Pseudomonas aeruginosa.


RESULTS
The probe synthesized was highly specific to Pseudomonas aeruginosa without cross reaction with other bacteria, viruses or fungi. The method was capable of detecting 100 ng bacteria DNA.


CONCLUSIONS
Reverse dot-blot hybridization possesses the merits of speediness and specificity in the detection of Pseudomonas aeruginosa in the early stage of infection.
Fan
Hui-zhen
HZ
Department of Respiratory Diseases, Guangzhou General Hospital of Guangzhou Command, Guangzhou 510010, China. huizhen2000@163.com
Huang
Wen-jie
WJ
Liang
Kun
K
Fang
Yi
Y
Ma
Li-ren
LR
Liu
Yao-qing
YQ
D001426:Bacterial Proteins / Q000235:genetics*; D008074:Lipoproteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D012680:Sensitivity and Specificity
D001426:Bacterial Proteins; D008074:Lipoproteins; C060507:outer membrane lipoprotein I, Bacteria