Characterizing the new transcription regulator protein p60TRP.
Active cell death ('apoptosis' or 'programmed cell death') is essential in the development and homeostasis of multicellular organisms and abnormal inhibition of apoptosis is an indicator of cancer and autoimmune diseases, whereas excessive cell death might be implicated in neurodegenerative disorders such as Alzheimer's disease (AD). Using bioinformatics-, Western-blotting-, yeast-two-hybrid-system-, polymerase chain reaction (PCR)-, and fluorescence microscopy-analyses, we demonstrate here that the neuroprotective protein p60TRP (p60-transcription-regulator-protein) is a basic helix-loop-helix (bHLH) domain-containing member of a new protein family that interacts with the Ran-binding-protein-5 (RanBP5) and the protein-phosphatase-2A (PP2A). The additional findings of its influence on NNT1 and p48ZnF (new-neurotrophin-1, p48-zinc-finger-protein)-signaling and its down-regulation in the brain of AD subjects point to a possible pivotal role of p60TRP in the control of cellular aging and survival.
Heese
K
K
BF Research Institute, c/o National Cardiovascular Center, 5-7-1 Fujishirodai, Suita, Osaka 565-0873, Japan. heesek@silver.ocn.ne.jp
Yamada
T
T
Akatsu
H
H
Yamamoto
T
T
Kosaka
K
K
Nagai
Y
Y
Sawada
T
T
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D051792:Basic Helix-Loop-Helix Proteins; D015139:Blotting, Southern; D001921:Brain / Q000378:metabolism; D016466:CHO Cells; D002454:Cell Differentiation / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D006224:Cricetinae; D003412:Cricetulus; D003593:Cytoplasm / Q000737:chemistry; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D015870:Gene Expression / Q000235:genetics; D020869:Gene Expression Profiling; D015723:Gene Library; D005822:Genetic Vectors / Q000235:genetics; D018257:Helix-Loop-Helix Motifs / Q000235:genetics; D006801:Humans; D008297:Male; D008954:Models, Biological; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000737:chemistry; D009206:Myocardium / Q000737:chemistry; D020932:Nerve Growth Factor / Q000494:pharmacology; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism; D016716:PC12 Cells; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D020798:Two-Hybrid System Techniques; D028961:beta Karyopherins / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled
D051792:Basic Helix-Loop-Helix Proteins; D018076:DNA, Complementary; D020932:Nerve Growth Factor; D009414:Nerve Growth Factors; D010749:Phosphoprotein Phosphatases; D034741:RNA, Small Interfering; D014157:Transcription Factors; D028961:beta Karyopherins; C496842:GPRASP3 protein, human; D043562:Receptors, G-Protein-Coupled; C497013:Bhlhb9 protein, rat; C107940:IPO5 protein, human