Photochemical immobilization of proteins on microwave-synthesized photoreactive polymers.
We report a rapid and versatile procedure for the preparation of photoreactive polymers and light-induced immobilization of proteins onto such polymers. Photoreactive controlled-pore glass, silica gel, glass slide, and polystyrene microtiter plate are prepared in 40-60s by microwave irradiation of the respective amino polymers and 1-fluoro-2-nitro-4-azidobenzene. Azido group, now part of the polymer, yields highly reactive nitrene under ultraviolet (UV) light at 365 nm. Thus, when photoreactive polymer and horseradish peroxidase or glucose oxidase are exposed to UV light, the reactive nitrene immobilizes the protein molecules in 10 to 20 min through covalent bonding. As nitrene has a property of inserting into C-H bond, the method may find potential applications for immobilization of biomolecules irrespective of their functional groups.
Naqvi
Azmi
A
Institute of Genomics and Integrative Biology (formerly, Centre for Biochemical Technology), Delhi University Campus, Mall Road, Delhi 110007, India.
Nahar
Pradip
P
D001386:Azides / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry / Q000528:radiation effects; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000528:radiation effects; D008872:Microwaves; D009578:Nitrobenzenes / Q000737:chemistry; D010777:Photochemistry / Q000379:methods*; D011108:Polymers / Q000138:chemical synthesis* / Q000528:radiation effects*; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry; D014466:Ultraviolet Rays
C492759:1-fluoro-2-nitro-4-azidobenzene; D001386:Azides; D004800:Enzymes, Immobilized; D009578:Nitrobenzenes; D011108:Polymers; D058428:Silica Gel; C014747:phenylazide; D012822:Silicon Dioxide; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase