Arabidopsis RAV1 is down-regulated by brassinosteroid and may act as a negative regulator during plant development.
RAV1 is a novel DNA-binding protein with two distinct DNA-binding domains unique in higher plants, but its role in plant growth and development remains unknown. Using cDNA array, we found that transcription of RAV1 is down-regulated by epibrassinolide (epiBL) in Arabidopsis suspension cells. RNA gel blot analysis revealed that epiBL-regulated RAV1 transcription involves neither protein phosphorylation/dephosphorylation nor newly synthesized protein, and does not require the functional BRI1, suggesting that this regulation might be through a new BR signaling pathway. Overexpressing RAV1 in Arabidopsis results in a retardation of lateral root and rosette leaf development, and the underexpression causes an earlier flowering phenotype, implying that RAV1 may function as a negative regulatory component of growth and development.
Hu
Yu Xin
YX
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Wang
Yong Xong
YX
Liu
Xin Fang
XF
Li
Jia Yang
JY
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D060406:Brassinosteroids; D002777:Cholestanols / Q000494:pharmacology*; D003513:Cycloheximide / Q000494:pharmacology; D016373:DNA, Antisense / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D015536:Down-Regulation / Q000187:drug effects; D035264:Flowers / Q000254:growth & development; D018628:Gene Dosage; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D019319:Okadaic Acid / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000254:growth & development; D018517:Plant Roots / Q000254:growth & development; D030821:Plants, Genetically Modified; D011494:Protein Kinases / Q000378:metabolism; D012231:Rhizobium / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects; D019311:Staurosporine / Q000494:pharmacology; D013260:Steroids, Heterocyclic / Q000494:pharmacology*; D014170:Transformation, Genetic / Q000235:genetics; D015026:Zeatin / Q000494:pharmacology
D029681:Arabidopsis Proteins; D060406:Brassinosteroids; D002777:Cholestanols; D016373:DNA, Antisense; D004268:DNA-Binding Proteins; D010937:Plant Growth Regulators; C117695:RAV1 protein, Arabidopsis; D013260:Steroids, Heterocyclic; D019319:Okadaic Acid; D015084:2,4-Dichlorophenoxyacetic Acid; D015026:Zeatin; D003513:Cycloheximide; D011494:Protein Kinases; C108270:BRI1 protein, Arabidopsis; D019311:Staurosporine; C023623:brassinolide