title: [Suppression of neutrophil immune function in rabbit models of hemorrhagic shock complicated by intra-abdominal hypertension].
abstract: OBJECTIVE
  To investigate the effects of hemorrhagic shock and intra-abdominal hypertension (IAH) on inflammatory responses of peripheral circulating neutrophils such as intracellular cytokine production, phagocytic capacity and expression of nuclear factor (NF)- kappaB.
  
  
  METHODS
  Twenty-four rabbits were divided equally into 4 groups including a hemorrhagic shock (HS) group complicated by abdominal compartment syndrome (ACS) (Group A), a HS group (Group B), a ACS group (Group C) and a normal control group (Group D). Intracellular interleukin (IL)-8 production in the peripheral neutrophils were measured in the rabbits by flow cytometry, phagocytic function of the neutrophils evaluated by a chemiluminescence method and the NF-kappaB expression detected by immunocytochemistry before, immediately and 4 h after the traumatization.
  
  
  RESULTS
  Four hours after the trauma, decreased intracellular IL-8 production and impaired phagocytic function of the peripheral neutrophils were observed in Group A along with suppressed NF-kappaB expression. But in Group B and Group C, the intracellular IL-8 production, phagocytic function and expression of NF-kappaB returned to the normal levels 4 hours after the trauma following the early-stage changes. In Group D, no significant changes occurred during the observation.
  
  
  CONCLUSIONS
  Responsiveness and function of the neutrophils to the stimuli by endotoxin are suppressed by the sequential second-hit of IAH after hemorrhagic shock, which may contribute to the occurrence of sepsis in ACS.
authors.affiliation: Department of Orthopaedics, Fuzhou General Hospital of Nanjing Command, Fuzhou 350000 China. androluqi@hotmail.com
authors.forename: Qi
authors.initials: Q
authors.lastname: Lü
authors.forename: Yan-feng
authors.initials: YF
authors.lastname: Zhuang
authors.forename: Wan-ming
authors.initials: WM
authors.lastname: Wang
authors.forename: Xue-ming
authors.initials: XM
authors.lastname: Cheng
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Sakamoto
authors.forename: Sayoko
authors.initials: S
authors.lastname: Takahara
authors.forename: Masamichi
authors.initials: M
authors.lastname: Nishida
authors.forename: Kunio
authors.initials: K
authors.lastname: Kobayashi
mesh_terms: D000005:Abdomen; D000818:Animals; D003161:Compartment Syndromes / Q000276:immunology*; D006973:Hypertension / Q000276:immunology; D016209:Interleukin-8 / Q000096:biosynthesis; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology*; D011817:Rabbits; D012771:Shock, Hemorrhagic / Q000150:complications* / Q000276:immunology*
chemical_list: D016209:Interleukin-8; D016328:NF-kappa B