Normal p21Ras/MAP kinase pathway expression and function in PBMC from patients with polycystic ovary disease.
Polycystic ovary disease (PCOD) is associated with insulin resistance and increased prevalence of type II diabetes mellitus (T2DM). The p21Ras/MAP kinase is a major intracellular signaling pathway mediating insulin signaling in insulin responsive tissues. The expression, regulation and function of the p21Ras/MAP kinase pathway in PCOD patients were examined. Peripheral blood mononuclear cells (PBMC) were isolated from ten patients with PCOD and ten controls. The expression of p21Ras and its regulatory proteins; hSOS1 and p120GAP were studied. The basal and phytohemaglutinin (PHA) or insulin stimulated phosphorylation of MAP kinase was determined. Expression of p21Ras, and its regulatory proteins hSOS1 and p120GAP were similar in PCOD patients and controls. Basal, PHA and insulin stimulated phosphorylation of MAP kinase, were also comparable in the two groups as well as their PBMC proliferative response. These data indicate that the expression and overall function of the p21Ras/MAP kinase pathway remain intact in non-diabetic patients with PCOD.
Buchs
A
A
Department of Internal Medicine 'C', Assaf Harofeh Medical Center, Zerifin 70300, Israel.
Chagag
P
P
Weiss
M
M
Kish
E
E
Levinson
R
R
Aharoni
D
D
Rapoport
M J
MJ
D000328:Adult; D015153:Blotting, Western; D002455:Cell Division; D005260:Female; D006801:Humans; D007328:Insulin / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000201:enzymology*; D020935:MAP Kinase Signaling System; D010766:Phosphorylation; D010835:Phytohemagglutinins / Q000378:metabolism; D011085:Polycystic Ovary Syndrome / Q000201:enzymology* / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism*; D020837:SOS1 Protein / Q000378:metabolism; D015398:Signal Transduction; D020729:p120 GTPase Activating Protein / Q000378:metabolism
D007328:Insulin; D010835:Phytohemagglutinins; D020837:SOS1 Protein; D020729:p120 GTPase Activating Protein; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)