Antinociceptive effects of Zataria multiflora Boiss fractions in mice.
Activity-guided fractionation of methanol:chloroform (1:1) extract of Zataria multiflora Boiss was performed in order to investigate the active component(s) responsible for antinociceptive activity. Antinociceptive activity was evaluated using hot-plate and writhing tests. Morphine (10 mg/kg, i.p.) and diclofenac (10 mg/kg, i.p.) were used as positive controls. The methanol-chloroform (1:1) extract of the aerial parts of the plants was prepared and partitioned between methanol-water (9:1) and n-hexane. The more active hydroalcoholic fraction was concentrated and further partitioned between methanol-water (3:2) and chloroform. The hydroalcoholic layer showed a dose-dependent and significant activity compared to morphine and diclofenac. Further fractionation of the hydroalcoholic fraction on silica gel gave six fractions. Three fractions at 100 mg/kg showed activity comparable to those of morphine.
Ramezani
Mohammad
M
Pharmaceutical Research Center, Bu-Ali Research Institute and School of Pharmacy, Mashhad University of Medical Sciences, PO Box 91775-1365, Mashhad, Iran. ramez_ani@hotmail.com
Hosseinzadeh
Hossein
H
Samizadeh
Shokoufeh
S
D000700:Analgesics / Q000302:isolation & purification / Q000627:therapeutic use*; D000818:Animals; D008297:Male; D051379:Mice; D010146:Pain / Q000188:drug therapy*; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*
D000700:Analgesics; D010936:Plant Extracts