title: Response of the Pacific oyster Crassostrea gigas to hydrocarbon contamination under experimental conditions.
abstract: Hydrocarbon contamination perturbs the metabolism of the marine bivalve Crassostrea gigas. To understand the response of this organism to hydrocarbon exposure, a suppression subtractive hybridisation method was employed to characterise up- and down-regulated genes during hydrocarbon exposure. The number of differentially expressed gene sequences obtained via this method was 258. The expression of genes involved in hydrocarbon detoxification (cytochrome p4501A1-like protein, cytochrome b(5), flavin-containing monooxygenase 2 and glutathione S-transferase omega class), protection against oxidative stress (copper/zinc superoxide dismutase) and cell protection (heat shock protein 70 family) was analysed by RT-PCR. An increase in the mRNA level of all genes studied was observed. A quantification of HSP70 by Enzyme Linked Immunosorbent Assay (ELISA) showed a significant increase of this protein during exposure. This study provides a basis for studying hydrocarbon detoxification processes in marine bivalves, especially C. gigas.
authors.affiliation: Laboratoire des Sciences de l'Environnement Marin (LEMAR), UMR-CNRS 6539, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, Tech. Brest Iroise, Place Nicolas Copernic, 29280 Plouzané, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Boutet
authors.forename: Arnaud
authors.initials: A
authors.lastname: Tanguy
authors.forename: Dario
authors.initials: D
authors.lastname: Moraga
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005648:Fuel Oils / Q000032:analysis / Q000633:toxicity; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006838:Hydrocarbons / Q000032:analysis / Q000633:toxicity*; D010125:Ostreidae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013482:Superoxide Dismutase / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D018076:DNA, Complementary; D005648:Fuel Oils; D018840:HSP70 Heat-Shock Proteins; D006838:Hydrocarbons; D014874:Water Pollutants, Chemical; D012313:RNA; D004247:DNA; D013482:Superoxide Dismutase