Synthesis of selenium-derivatized cytidine and oligonucleotides for X-ray crystallography using MAD.
Synthesis of the novel 2'-Se-cytidine phosphoramidite was achieved via transformation of the uridine analogue to the cytidine derivative in high yield. This 2'-Se-cytidine phosphoramidite was used to synthesize selenium-derivatized DNA and RNA oligonucleotides for X-ray crystallography using MAD. The nucleotide coupling yield using this novel phosphoramidite was over 99% when 5-benzylmercaptotetrazole (5-BMT) was used as the coupling reagent.
Buzin
Yuri
Y
Department of Chemistry, Brooklyn College, The Graduate School, The City University of New York, 2900 Bedford Avenue, Brooklyn, New York 11210, USA.
Carrasco
Nicolas
N
Huang
Zhen
Z