Nanoassembly of biodegradable microcapsules for DNA encasing.
A new microcontainer for DNA delivery based on biocompatible poly[beta-glucuronic acid-(1 --> 3)-N-acetyl-beta-galactosamine-6-sulfate-(1 --> 4)](chondroitin sulfate)/poly(-l-arginine) microcapsules with 40 nm thick molecularly organized shell was proposed. DNA molecules were deposited as DNA/sperimidine complex on the surface of template 4 mum core particles followed by layer-by-layer nanoassembly of protective chondroitin sulfate/poly(-l-arginine) shell. After template core dissolution, DNA molecules were captured inside microcapsules retaining a natural double-helix structure. The developed DNA encapsulation approach can be employed for targeted delivery of plasmid DNA in living cells.
Shchukin
Dmitry G
DG
Institute for Micromanufacturing, Louisiana Tech University, Ruston, Louisiana 71272, USA.
Patel
Amish A
AA
Sukhorukov
Gleb B
GB
Lvov
Yuri M
YM
D001672:Biocompatible Materials / Q000737:chemistry; D002809:Chondroitin Sulfates / Q000737:chemistry*; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D004339:Drug Compounding; D018014:Gene Transfer Techniques; D008856:Microscopy, Fluorescence; D036103:Nanotechnology / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D013095:Spermidine / Q000737:chemistry
D001672:Biocompatible Materials; D010455:Peptides; C015462:polyarginine; D002809:Chondroitin Sulfates; D004247:DNA; D013095:Spermidine