Inhibitory action of a new lectin from Xerocomus chrysenteron on cell-substrate adhesion.
Lectins are carbohydrate-binding proteins which potentially link to cell surface glycoconjugates and affect cell proliferation. We investigated the effect of a new lectin from the mushroom Xerocomus chrysenteron (XCL) on cell proliferation using adherent and suspension cell lines. XCL caused a dose-dependent inhibition of proliferation of the adherent cell lines NIH-3T3 and HeLa. Several experiments suggest that disruption of cell-substrate adhesion is the main factor affecting cell growth inhibition. (i) No antiproliferative effect was observed on the SF9 cell line, which does not require to be attached to grow. (ii) XCL was shown to affect the adherence of cells following their suspension by trypsin treatment. (iii) XCL was localized on the cell surface where it would act as a coating agent. (iv) XCL induced morphological changes from well spread to rounded cells and disrupted the actin cytoskeleton. By contrast, flow cytometric analysis showed that XCL does not interfere with the cell cycle, and does not induce apoptosis.
Marty-Detraves
Claire
C
IPBS UMR5089, Biophysique et Biotechnologie des Protéines, Toulouse, France.
Francis
Frédéric
F
Baricault
Laurent
L
Fournier
Didier
D
Paquereau
Laurent
L
D008841:Actin Cytoskeleton / Q000378:metabolism; D000818:Animals; D001487:Basidiomycota / Q000737:chemistry* / Q000235:genetics; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003599:Cytoskeleton / Q000187:drug effects; D006001:Glycoconjugates / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000235:genetics / Q000494:pharmacology*; D051379:Mice; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology
D006001:Glycoconjugates; D037102:Lectins; D011994:Recombinant Proteins