Rescue of cells from apoptosis by antioxidants occurs downstream from GSH extrusion.
Antioxidants-that is, scavengers of free radicals and anaerobic conditions (5%CO(2), 95% N(2))-protect monocytic U937 cells from damage-induced apoptosis. Antioxidants rescue the cells acting on the apoptotic pathway at a step downstream from gluthatione extrusion. Reducing agents, such as DTT, also reduce stress-induced apoptosis. Thus, apoptotic GSH extrusion triggers the downstream events of apoptosis by leaving cells unprotected against thiol\s oxidation and radical production.
Liuzzi
Francesca
F
Dipartimento di Biologia, Cattedra Medicina del Lavoro, Universita di Roma Tor Vergata, 00133 Rome, Italy.
Fanelli
Claudia
C
Ciriolo
Maria Rosa
MR
Cerella
Claudia
C
D'Alessio
Maria
M
Denicola
Milena
M
Magrini
Andrea
A
Bergamaschi
Antonio
A
Ghibelli
Lina
L
D000693:Anaerobiosis; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D015687:Cell Hypoxia / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D005978:Glutathione / Q000502:physiology*; D006801:Humans; D011691:Puromycin / Q000494:pharmacology; D020298:U937 Cells
D000975:Antioxidants; D011691:Puromycin; D005978:Glutathione