Human cytomegalovirus (HCMV) infection in osteosarcoma cell line suppresses GM-CSF production by induction of TGF-beta.
This study was performed to elucidate the possible mechanism of the disturbance of hemopoiesis by HCMV infection. Saos-2 cells constitutively express mRNA of GM-CSF, and its expression was profoundly decreased by HCMV infection, which required full replication of the virus and was mediated by soluble factors released from the HCMV-infected Saos-2 cells. TGF-beta1 production was statistically and significantly increased from one day after HCMV infection. Expression and production of GM-CSF in Saos-2 cells were restored when a culture supernatant of HCMV-infected Saos-2 cells was reacted with neutralizing anti-TGF-beta antibody. Conclusively, HCMV inhibits GM-CSF expression in Saos-2 cells partly by the increased production of TGF-beta1.
Kwon
Ye Jin
YJ
Department of Microbiology and Immunology, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Republic of Korea.
Kim
Dae-Joong
DJ
Kim
Jung Heon
JH
Park
Chung-Gyu
CG
Cha
Chang-Yong
CY
Hwang
Eung-Soo
ES
D002460:Cell Line; D003587:Cytomegalovirus / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D006801:Humans; D012516:Osteosarcoma / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000502:physiology*; D014407:Tumor Cells, Cultured / Q000821:virology*; D014779:Virus Replication
D016212:Transforming Growth Factor beta; D016178:Granulocyte-Macrophage Colony-Stimulating Factor