Expression and functional characterization of the system L amino acid transporter in KB human oral epidermoid carcinoma cells.
We have examined the expression and function of system l amino acid transporter in KB human oral epidermoid carcinoma cells. The KB cells express l-type amino acid transporter 1 (LAT1) in plasma membrane, but not l-type amino acid transporter 2 (LAT2). The [14C]l-leucine uptake by KB cells is inhibited by system l selective inhibitor BCH. The majority of [14C]l-leucine uptake is, therefore, mediated by LAT1. These results suggest that the transport of neutral amino acids including several essential amino acids into the KB cells mediated by LAT1 and the specific inhibition of LAT1 in oral cancer cells will be a new rationale for anti-cancer therapy.
Yoon
Jung Hoon
JH
Oral Biology Research Institute, Chosun University College of Dentistry, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, South Korea.
Kim
Youn Bae
YB
Kim
Myong Soo
MS
Park
Joo Cheol
JC
Kook
Joong Ki
JK
Jung
Hae Man
HM
Kim
Saeng Gon
SG
Yoo
Hoon
H
Ko
Yeong Mu
YM
Lee
Sang Ho
SH
Kim
Bong Young
BY
Chun
Hong Sung
HS
Kanai
Yoshikatsu
Y
Endou
Hitoshi
H
Kim
Do Kyung
DK
D027182:Amino Acid Transport System y+; D000598:Amino Acids, Cyclic / Q000494:pharmacology; D027301:Fusion Regulatory Protein 1, Light Chains / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007624:KB Cells / Q000737:chemistry*; D027283:Large Neutral Amino Acid-Transporter 1 / Q000032:analysis* / Q000502:physiology; D007930:Leucine / Q000378:metabolism
D027182:Amino Acid Transport System y+; D000598:Amino Acids, Cyclic; D027301:Fusion Regulatory Protein 1, Light Chains; D027283:Large Neutral Amino Acid-Transporter 1; C420646:SLC7A8 protein, human; C006504:2-aminobicyclo(2,2,1)heptane-2-carboxylic acid; D007930:Leucine