title: NY-ESO-1 expression and immunogenicity in malignant and benign breast tumors.
abstract: NY-ESO-1 is a cancer/testis antigen expressed in normal adult tissues solely in the testicular germ cells of normal adults and in various cancers. It induces specific humoral and cellular immunity in patients with NY-ESO-1-expressing cancer. The aim of this study was to determine the frequency of NY-ESO-1 mRNA and protein expression in malignant and benign breast tumors. NY-ESO-1 mRNA expression was detected by conventional reverse transcription-PCR and real-time PCR, and that of the protein expression by immunohistochemistry and Western blot analysis. Expression of NY-ESO-1 mRNA was detected in 37 of 88 (42%) cancer specimens, whereas that of the NY-ESO-1 protein was detected only in 1 mRNA-positive specimen. In the latter case, expression level of NY-ESO-1 mRNA relative to that in the testis was relatively high (75% of testicular expression) and to the other among breast cancer specimens. In benign breast lesions, 21 of 31 (68%) specimens expressed low levels of NY-ESO-1 mRNA. In 1 case of fibroadenoma, NY-ESO-1 mRNA was 8% of the testicular level, and protein was detected by Western blot analysis. Only 1 breast cancer patient had detectable antibody at time of surgery, which disappeared within 2 years. Tumor specimen from this patient was both NY-ESO-1 mRNA and protein positive, and NY-ESO-1-specific CD8 T cells were detected in this patient by IFN-gamma enzyme-linked immunospot assay using NY-ESO-1 recombinant adeno and vaccinia virus. A higher rate of NY-ESO-1 expression was noted in breast cancer with high histological grade and negative hormone receptor status, suggesting NY-ESO-1 as a potential tumor antigen for immunotherapy in patients with breast cancer and poor prognosis.
authors.affiliation: Department of Surgery and Clinical Oncology, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita-city, Osaka 565-0871, Japan.
authors.forename: Yurika
authors.initials: Y
authors.lastname: Sugita
authors.forename: Hisashi
authors.initials: H
authors.lastname: Wada
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Fujita
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Nakata
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Sato
authors.forename: Yuji
authors.initials: Y
authors.lastname: Noguchi
authors.forename: Achim A
authors.initials: AA
authors.lastname: Jungbluth
authors.forename: Masae
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Yao-Tseng
authors.initials: YT
authors.lastname: Chen
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Stockert
authors.forename: Sacha
authors.initials: S
authors.lastname: Gnjatic
authors.forename: Barbara
authors.initials: B
authors.lastname: Williamson
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Scanlan
authors.forename: Toshiro
authors.initials: T
authors.lastname: Ono
authors.forename: Isao
authors.initials: I
authors.lastname: Sakita
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Yasui
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Miyoshi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tamaki
authors.forename: Nariaki
authors.initials: N
authors.lastname: Matsuura
authors.forename: Shinzaburo
authors.initials: S
authors.lastname: Noguchi
authors.forename: Lloyd J
authors.initials: LJ
authors.lastname: Old
authors.forename: Eiichi
authors.initials: E
authors.lastname: Nakayama
authors.forename: Morito
authors.initials: M
authors.lastname: Monden
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000328:Adult; D000912:Antibodies, Neoplasm / Q000097:blood; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000276:immunology*; D001943:Breast Neoplasms / Q000235:genetics* / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes; D044584:Carcinoma, Ductal / Q000235:genetics / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008212:Lymphocyte Depletion; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology*; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014616:Vaccinia virus / Q000235:genetics
chemical_list: D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; C104948:CTAG1B protein, human; D008565:Membrane Proteins; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D011994:Recombinant Proteins