title: Rescue of cells from apoptosis by antioxidants occurs downstream from GSH extrusion.
abstract: Antioxidants-that is, scavengers of free radicals and anaerobic conditions (5%CO(2), 95% N(2))-protect monocytic U937 cells from damage-induced apoptosis. Antioxidants rescue the cells acting on the apoptotic pathway at a step downstream from gluthatione extrusion. Reducing agents, such as DTT, also reduce stress-induced apoptosis. Thus, apoptotic GSH extrusion triggers the downstream events of apoptosis by leaving cells unprotected against thiol\s oxidation and radical production.
authors.affiliation: Dipartimento di Biologia, Cattedra Medicina del Lavoro, Universita di Roma Tor Vergata, 00133 Rome, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Liuzzi
authors.forename: Claudia
authors.initials: C
authors.lastname: Fanelli
authors.forename: Maria Rosa
authors.initials: MR
authors.lastname: Ciriolo
authors.forename: Claudia
authors.initials: C
authors.lastname: Cerella
authors.forename: Maria
authors.initials: M
authors.lastname: D'Alessio
authors.forename: Milena
authors.initials: M
authors.lastname: Denicola
authors.forename: Andrea
authors.initials: A
authors.lastname: Magrini
authors.forename: Antonio
authors.initials: A
authors.lastname: Bergamaschi
authors.forename: Lina
authors.initials: L
authors.lastname: Ghibelli
mesh_terms: D000693:Anaerobiosis; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D015687:Cell Hypoxia / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D005978:Glutathione / Q000502:physiology*; D006801:Humans; D011691:Puromycin / Q000494:pharmacology; D020298:U937 Cells
chemical_list: D000975:Antioxidants; D011691:Puromycin; D005978:Glutathione