The development and application of methods for activity-based protein profiling.
Recent advances in genomic and proteomic technologies have begun to address the challenge of assigning molecular and cellular functions to the numerous protein products encoded by prokaryotic and eukaryotic genomes. In particular, chemical strategies for proteome analysis have emerged that enable profiling of protein activity on a global scale. Herein, we highlight these chemical proteomic methods and their application to the discovery and characterization of disease-related enzyme activities.
Jessani
Nadim
N
The Skaggs Institute for Chemical Biology and Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Cravatt
Benjamin F
BF
D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004798:Enzymes / Q000378:metabolism; D006801:Humans; D009369:Neoplasms / Q000201:enzymology / Q000378:metabolism; D010963:Plasmodium falciparum / Q000502:physiology; D040081:Protein Array Analysis / Q000379:methods*; D020543:Proteome / Q000378:metabolism
D004791:Enzyme Inhibitors; D004798:Enzymes; D020543:Proteome