Semi-automated 86Y purification using a three-column system.
The separation of (86)Y from (86)Sr was optimized by a semi-automated purification system involving the passage of the target sample through three sequential columns. The target material was dissolved in 4 N HNO(3) and loaded onto a Sr-selective (Sr-Spec) column to retain the (86)Sr. The yttrium was eluted with 4 N HNO(3) onto the second Y-selective (RE-Spec) column with quantitative retention. The RE-Spec column was eluted with a stepwise decreasing concentration of HNO(3) to wash out potential metallic impurities to a waste container. The eluate was then pumped onto an Aminex A5 column with 0.1 N HCl and finally with 3 N HCl to collect the radioyttrium in 0.6-0.8 mL with a >80% recovery. This method enabled us to decontaminate Sr by 250,000 times and label 30 micro g of DOTA-Biotin with a >95% yield.
Park
Luke S
LS
Nuclear Medicine Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA.
Szajek
Lawrence P
LP
Wong
Karen J
KJ
Plascjak
Paul S
PS
Garmestani
Kayhan
K
Googins
Shawn
S
Eckelman
William C
WC
Carrasquillo
Jorge A
JA
Paik
Chang H
CH
D001710:Biotin / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry*; D002845:Chromatography / Q000295:instrumentation* / Q000379:methods*; D007553:Isotope Labeling / Q000379:methods*; D009942:Organometallic Compounds / Q000032:analysis / Q000737:chemistry*; D011485:Protein Binding; D019275:Radiopharmaceuticals / Q000138:chemical synthesis; D012371:Robotics / Q000379:methods*; D013325:Strontium Isotopes / Q000302:isolation & purification*; D015021:Yttrium Radioisotopes / Q000737:chemistry* / Q000302:isolation & purification*
C405616:DOTA-biotin; D009942:Organometallic Compounds; D019275:Radiopharmaceuticals; D013325:Strontium Isotopes; D015021:Yttrium Radioisotopes; D001710:Biotin