title: Canine prostate carcinomas express markers of urothelial and prostatic differentiation.
abstract: Prostate carcinoma and transitional cell carcinoma (TCC) occur in the prostate gland of older dogs and have morphologic similarities when evaluated by light microscopy. The dog is a commonly used animal model for studying human prostate carcinoma; therefore, it is important to accurately differentiate canine prostate carcinomas from TCCs. We investigated whether keratin 7 (K7) and arginine esterase (AE) would aid differentiation of canine prostate carcinoma from TCC. K7 expression was evaluated in normal and neoplastic canine prostate and bladder tissues using immunohistochemistry. The expression of AE messenger ribonucleic acid (mRNA) in normal and neoplastic canine prostate and bladder was detected using northern blots and reverse transcriptase-polymerase chain reaction (RT-PCR). In addition, AE enzyme activity was measured in normal and neoplastic canine prostate and bladder tissues. We found marked similarities in K7 expression in prostate carcinomas and TCCs. AE mRNA was present in high levels in normal prostatic tissue but was reduced in prostate carcinoma by northern blot assay. Nested RT-PCR detected AE mRNA both in TCCs (13 of 15) and in prostate carcinomas (13 of 13). Enzymatic activity of AE was high in normal prostate gland and in some prostate carcinomas, whereas normal bladder and TCCs produced lower levels of AE. In conclusion, K7 and AE cannot be used to differentiate TCC from prostate carcinoma in dogs.
authors.affiliation: Department of Pathology, University of Georgia, Athens, USA.
authors.forename: B E
authors.initials: BE
authors.lastname: LeRoy
authors.forename: M V P
authors.initials: MV
authors.lastname: Nadella
authors.forename: R E
authors.initials: RE
authors.lastname: Toribio
authors.forename: I
authors.initials: I
authors.lastname: Leav
authors.forename: T J
authors.initials: TJ
authors.lastname: Rosol
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism; D002295:Carcinoma, Transitional Cell / Q000175:diagnosis / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D017931:DNA Primers; D004283:Dog Diseases / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D004285:Dogs; D015870:Gene Expression / Q000235:genetics*; D007150:Immunohistochemistry; D053552:Keratin-7; D007633:Keratins / Q000378:metabolism; D008297:Male; D019048:Prostatic Intraepithelial Neoplasia / Q000175:diagnosis / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; C507497:KRT7 protein, human; D053552:Keratin-7; D012333:RNA, Messenger; D007633:Keratins; C019724:arginine esterase; D002265:Carboxylic Ester Hydrolases