[Ribosomal DNA ITS sequences analysis of the Chinese crude drug fructus schisandrae sphenantherae and fruts of Schisandra viridis].
OBJECTIVE
To find the patterns of the rDNA ITS sequence variation of Schisandra sphenanthera and S. viridis, and to establish the molecular biological method for the identification of Fructus Schisandrae Sphenantherae and the fruits of S. viridis.


METHODS
PCR products were sequenced directly and the sequences were analyzed with PAUP 4.0b10. NJ systematic tree was obtained with neighbor-joining method.


RESULTS
The Complete ITS sequence of S. sphenanthera was 691-692 bp, of which there were 282 bp of ITS1 and 246-247 bp of ITS2. The complete sequence of S. viridis was 694-695 bp, consisting of 285-286 bp of ITS1 and 246-247 bp of ITS2. There were three informative sites in ITS1 regions for the two species. In the NJ tree with Kadsura anamosma and K. coccinea as outgroups, five different populations of S. viridis were the monophyletic group with the bootstrap value of 68%. These populations included one from Tianmushan, Zhejiang province, three populations from Jigongshan, Henan Province and the other two populations of S. viridis cited the sequences from GeneBank (registration numbers are AF263438 and AF163703 respectively).


CONCLUSIONS
The rDNA internal transcribed spacer is a good marker to distinguish the Fructus Schisandrae Sphenantherae from the fruits of S. viridis.
Gao
Jian-ping
JP
Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 200032, China.
Wang
Yan-han
YH
Qiao
Chun-feng
CF
Chen
Dao-feng
DF
D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D004340:Drug Contamination; D005638:Fruit / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics*; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics*; D032141:Schisandra / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA
D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer; D012340:RNA, Ribosomal, 5.8S