Hepatic acute-phase reaction in histamine-deficient gene targeted mice.
Histamine is a versatile mediator that, according to in vitro studies, affects the synthesis of inflammatory cytokines and acute-phase proteins. The histidine-decarboxylase knockout (HDC-/-) mouse is a model of in vivo investigation of the physiologic and metabolic integration of the acutephase response. These mice do not synthesise histamine and feeding them with histamine-poor diet they are almost completely histamine-deficient. We compared the serum concentrations of representatives of acute-phase plasma proteins, as well as the levels IL-6 and IL-1alpha in wild type and HDC-/- mice during local (turpentine-induced) or systemic (LPS-induced) inflammation. The level of some acute-phase proteins significantly differed in wild-type and HDC-/- mice while others remained unaffected. The IL-6 levels are also differ in the wild-type and histamine-deficient animals, suggesting that the effect of histamine is attained through IL-6, although direct effect is not disclosed yet.
Donászi-Ivanov
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Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Hungary.
Scharek
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P
Falus
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Fülöp
A K
AK
D000209:Acute-Phase Proteins / Q000032:analysis*; D000818:Animals; D003176:Complement C3 / Q000032:analysis; D006632:Histamine / Q000502:physiology*; D006640:Histidine Decarboxylase / Q000172:deficiency / Q000235:genetics; D007375:Interleukin-1 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D051379:Mice; D000683:Serum Amyloid P-Component / Q000032:analysis; D000511:alpha-Macroglobulins / Q000032:analysis
D000209:Acute-Phase Proteins; D003176:Complement C3; D007375:Interleukin-1; D015850:Interleukin-6; D000683:Serum Amyloid P-Component; D000511:alpha-Macroglobulins; D006632:Histamine; D006640:Histidine Decarboxylase