Novel testis- and embryo-specific isoforms of the phosphofructokinase-1 muscle type gene.
We have identified novel transcriptional isoforms of the human and mouse genes encoding muscle type phosphofructokinase-1 (PFK-M). These isoforms are expressed specifically in the testis and in the mid-gestation embryo, and have been termed TE-PFK-M (testis- and embryo-specific PFK-M). The 5'UTR of TE-PFK-M is composed of three newly identified exons that lie much farther upstream of the PFK-M coding region than the previously characterized 5'UTR. In addition, this upstream region encodes a series of small polyadenylated transcripts, some of which share the same exons found in the 5'UTR of TE-PFK-M, and which may play some role in regulating TE-PFK-M expression. These findings indicate an even more complex level of control of PFK-M expression than previously thought.
Yamada
Satoru
S
Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, National Cancer Institute, NCI-Frederick, Frederick, MD 21702, USA.
Nakajima
Hiromu
H
Kuehn
Michael R
MR
D000818:Animals; D018076:DNA, Complementary / Q000302:isolation & purification; D004622:Embryo, Mammalian / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D051379:Mice; D016254:Mutagenesis, Insertional; D025203:Phosphofructokinase-1, Muscle Type / Q000096:biosynthesis / Q000235:genetics*; D011533:Proviruses / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D013737:Testis / Q000201:enzymology*; D014018:Tissue Distribution; D024363:Transcription Initiation Site
D018076:DNA, Complementary; D007527:Isoenzymes; D012333:RNA, Messenger; D025203:Phosphofructokinase-1, Muscle Type