title: In vitro hematopoiesis produces a distinct class of immature dendritic cells from spleen progenitors with limited T cell stimulation capacity.
abstract: The study of dendritic cells (DC) has been hampered by the difficulty of isolating rare cells for analysis of their phenotype and function. Interpretation of the DC lineage has been largely influenced by studies on cell populations which can be readily isolated and amplified in the presence of cytokines. Long term cultures (LTC) from murine spleen have been shown to support continuous in vitro hematopoiesis of DC dependent on interaction with a stromal cell monolayer. LTC-DC represent a single, stable class of DC derived by constant turnover of spleen DC progenitors maintained within stroma. They represent a resident DC population in spleen. The functional characteristics of LTC-DC have been studied in terms of capacity to stimulate T cells and response to activation by environmental stimuli. LTC-DC have many morphological, phenotypic and functional properties reflecting an immature or partially mature, marginal zone-like CD4(-)CD8(-) splenic DC subset. They are highly endocytic and can process and present protein antigen to naive hen egg lysozyme (HEL)-specific MHC-II-restricted TCR-Tg CD4(+) T cells. They do not, however, induce T cell proliferation in a mixed lymphocyte reaction. LTC-DC do not respond in a typical fashion to common DC activators like LPS and CD40L. They upregulate MHC-I and CD80/CD86 but not MHC-II and CD40. They reflect an endogenous, immature DC subset in spleen with properties distinct from immature DC located in peripheral tissues.
authors.affiliation: School of Biochemistry and Molecular Biology, Australian National University, Canberra, ACT 0200, Australia.
authors.forename: Ben
authors.initials: B
authors.lastname: Quah
authors.forename: Keping
authors.initials: K
authors.lastname: Ni
authors.forename: Helen C
authors.initials: HC
authors.lastname: O'Neill
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D000954:Antigens, Surface / Q000032:analysis; D051940:B7-2 Antigen; D017252:CD3 Complex / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D019013:CD40 Antigens / Q000378:metabolism; D023201:CD40 Ligand / Q000378:metabolism / Q000494:pharmacology; D002454:Cell Differentiation / Q000276:immunology / Q000502:physiology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology* / Q000648:ultrastructure; D004705:Endocytosis / Q000502:physiology; D005260:Female; D005434:Flow Cytometry; D006411:Hematopoiesis, Extramedullary / Q000276:immunology* / Q000502:physiology; D006649:Histocompatibility Antigens / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D037181:Lectins, C-Type; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic / Q000235:genetics / Q000276:immunology; D046529:Microscopy, Electron, Transmission; D018448:Models, Immunological; D009113:Muramidase / Q000276:immunology / Q000494:pharmacology; D010047:Ovalbumin / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D051940:B7-2 Antigen; D017252:CD3 Complex; D019013:CD40 Antigens; D023201:CD40 Ligand; D006649:Histocompatibility Antigens; D000949:Histocompatibility Antigens Class II; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D009113:Muramidase; D010047:Ovalbumin; D000099192:CD69 Antigens; C485468:Cd3e protein, mouse; C497965:Cd86 protein, mouse; C421864:hen egg lysozyme