title: Crystallization and preliminary X-ray analysis of two inhibitor complexes of the catalytic domain of death-associated protein kinase.
abstract: The catalytic domain of death-associated protein kinase (DAPK) has been overexpressed, purified and crystallized using the sitting-drop vapour-diffusion method with PEG 8000 and magnesium acetate as precipitants. Complexes with the inhibitor staurosporine and its analogue BDB402 were also crystallized in the presence of PEG 400 and PEG 8000, respectively. Diffraction data were collected to 2.4 A for the native catalytic domain, to 2.9 A for the staurosporine complex and to 2.7 A for the BDB402 complex. All three crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 77.992, b = 109.909, c = 50.063 A for the catalytic domain, a = 78.911, b = 113.162, c = 50.658 A for the staurosporine complex and a = 77.337, b = 108.869, c = 50.186 A for the BDB402 complex. In both complexes the inhibitor molecule was clearly assigned in the difference Fourier map calculated on the basis of the phases obtained from the structure of the catalytic domain.
authors.affiliation: Devision of Chemistry, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Yamakawa
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ogata
authors.forename: Kumiko
authors.initials: K
authors.lastname: Morita
authors.forename: Naoki
authors.initials: N
authors.lastname: Shibata
authors.forename: Naoko
authors.initials: N
authors.lastname: Andou
authors.forename: Hiromi
authors.initials: H
authors.lastname: Sanuki
authors.forename: Kazunori
authors.initials: K
authors.lastname: Yamada
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hioki
authors.forename: Takehisa
authors.initials: T
authors.lastname: Ishii
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Higuchi
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000737:chemistry*; D020134:Catalytic Domain; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D064550:Death-Associated Protein Kinases; D004791:Enzyme Inhibitors / Q000737:chemistry*; D006801:Humans; D019311:Staurosporine / Q000737:chemistry
chemical_list: D051017:Apoptosis Regulatory Proteins; D004791:Enzyme Inhibitors; D064550:Death-Associated Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D019311:Staurosporine