title: [The relationship with human papilloma virus DNA in cervical adenocarcinoma].
abstract: Recently, cervical adenocarcinoma has been increasing especially among young women and account for 10-20% of cervical cancer. However, the detection rate of HPV-DNA was 35-85% and lower than that of squamous cell carcinoma. Furthermore, the relationship with HPV in cervical adenocarcinoma was not much investigated in Japan, so we studied HPV status in cervical adenocarcinoma by in situ PCR method using biotin-labeled DNA probes, because in situ PCR method possesses the advantages of both PCR and in situ hybridization in being highly sensitive and enabling visualization of the cellular localization of the DNA. HPV infection was analyzed in 60 cervical adenocarcinomas, including 1 adenocarcinoma in situ and 15 adenosquamous carcinomas. HPV-DNA was detected in 47 of all 60 cases(78%): 35 of 45(78%) in adenocarcinomas and 12 of 15(80%) in adenosquamous carcinomas. No significant correlation was found between the HPV-DNA detection rate and histological subtypes of adenocarcinoma. In conclusion, cervical adenocarcinoma demonstrates a high prevalence of HPV-DNA as well as other previous studies. Therefore, HPV infection plays a very important role in not only squamous cell carcinoma but also adenocarcinoma in uterine cervix.
authors.affiliation: Department of Pathology, Juntendo University School of Medicine, Bunkyo-Ku, Tokyo 113-8421.
authors.forename: Kanako
authors.initials: K
authors.lastname: Ogura
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Ishi
authors.forename: Fujihiko
authors.initials: F
authors.lastname: Suzuki
authors.forename: Hiroko
authors.initials: H
authors.lastname: Suto
authors.forename: Katsunari
authors.initials: K
authors.lastname: Kina
authors.forename: Junichi
authors.initials: J
authors.lastname: Koyatsu
authors.forename: Michio
authors.initials: M
authors.lastname: Nojima
authors.forename: Koyo
authors.initials: K
authors.lastname: Yoshida
authors.forename: Koichi
authors.initials: K
authors.lastname: Suda
mesh_terms: D000230:Adenocarcinoma / Q000209:etiology* / Q000821:virology; D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000150:complications*; D016133:Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000209:etiology* / Q000821:virology
chemical_list: D004279:DNA, Viral