title: Molecular cloning and characterization of a novel rat CXC chemokine, rPBP, the homologue of human and mouse PBP.
abstract: We have previously cloned the mouse platelet basic protein (mPBP), a homologue of human PBP, from mouse thymic stromal cells. Using EST alignment and RT-PCR, the rat homologue of human and mouse PBP was cloned from lung and named as rPBP. The complete open reading frame and part of the 3'- and 5'-non-coding regions were obtained through rapid amplification of cDNA ends. The rPBP cDNA encodes a protein of 111 amino acids containing a signal peptide of 37 amino acids at the N-terminus, with the mature protein of 74 amino acids. The rPBP is a new member of ELR+CXC chemokines. The mature protein of rPBP shares 69% and 45% homology with mouse and human PBP, respectively. In situ hybridization assay revealed rPBP to be predominantly localized in the pulmonary vascular endothelial cells. The eukaryotic expression vector pCDNA3-rPBP was constructed and transiently transfected into COS-7 cells. In the in vitro chemotaxis assay, the polymorphonuclear leukocytes (PMNs) were chemoattracted to the supernatants from transfected COS-7 cells in a dose-dependent manner. The implication of rPBP found in rat lung is that this chemokine may have the function to recruit PMNs to fight against pulmonary infection.
authors.affiliation: Department of Immunology, Peking University Health Science Center, Beijing 100083, China.
authors.forename: Wen-Xian
authors.initials: WX
authors.lastname: Fu
authors.forename: Meng-Lei
authors.initials: ML
authors.lastname: Zhu
authors.forename: Shun-You
authors.initials: SY
authors.lastname: Gong
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Wei-Feng
authors.initials: WF
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D019743:Chemokines, CXC / Q000737:chemistry / Q000235:genetics*; D002634:Chemotaxis, Leukocyte; D002522:Chlorocebus aethiops; D033481:Chromosomes, Mammalian / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D008168:Lung / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D016415:Sequence Alignment; D017385:Sequence Homology
chemical_list: D019743:Chemokines, CXC; D018076:DNA, Complementary; C493799:Ppbp protein, rat; D012333:RNA, Messenger