[Preservation of germplasm of Rehmannia glutinosa in vitro].
OBJECTIVE
To establish a method for the germplasm preservation of R. glutinosa.


METHODS
Plantlets of different cultivars obtained by tip culture were inoculated into test tubes with MS medium supplemented with 0.2 mg.L-1 BA and 0.02 mg.L-1 NAA, and preserved at 4-6 degrees C in dark. At the same time, different media (A. distilled water + 10 g.L-1 agar; B. 1/2 MS + 5 g.L-1 agar; C. MS + 10 g.L-1 agar; D. 1/2 MS + 0.5 BA + 0.02 NAA + 10 g.L-1 agar; E. MS + 0.5 BA + 0.02 NAA + 10 g.L-1 agar) were set to conserve plantlets of "85-5" on the same condition.


RESULTS
4 months later, the death rate of "Xinggeda" was 73%, "Tucheng" 60%, "85-5" 33%, and "Beijing 1" 9%. All of the plantlets of "85-5" in different media kept alive. 10 months later, most of the preserved plantlets browned and wilted except those on medium A.


CONCLUSIONS
The low temperature endurance of R. glutinosa is cultivar-dependent. Medium A can preserve "85-5" for more than 10 months in vitro.
Wen
Xue-sen
XS
School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandon, China. x.s.wen@163.com
Wei
Jian-he
JH
Yang
Shi-lin
SL
Ma
Xiao-jun
XJ
D000362:Agar; D003470:Culture Media; D046508:Culture Techniques; D010946:Plants, Medicinal / Q000254:growth & development*; D032267:Rehmannia / Q000254:growth & development*; D013696:Temperature; D014021:Tissue Preservation / Q000379:methods*
D003470:Culture Media; D000362:Agar