Development and validation of a quantitative assay for raloxifene by capillary electrophoresis.
The migration behavior of raloxifene was investigated by capillary electrophoresis (CE). The influence of different parameters (nature and concentration of the running buffer, pH and applied voltage) on migration time, peak symmetry and efficiency was systematically investigated. A buffer consisting of 20mM acetate buffer of pH 4.5 was found to provide a very efficient and stable electrophoretic system for the analysis of raloxifene. The optimized method was validated with respect to precision, linearity, limits of detection and quantification, accuracy and robustness. The applicability of the assay was demonstrated by analyzing this drug in human plasma and pharmaceutical preparations.
Pérez-Ruiz
Tomás
T
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071 Murcia, Spain. tpr@fcu.um.es
Martínez-Lozano
Carmen
C
Sanz
Antonio
A
Bravo
Eva
E
D019075:Electrophoresis, Capillary / Q000379:methods; D020849:Raloxifene Hydrochloride / Q000032:analysis* / Q000737:chemistry / Q000592:standards*; D013678:Technology, Pharmaceutical / Q000379:methods*
D020849:Raloxifene Hydrochloride