Differentiation of isomeric oligosaccharide structures by ESI tandem MS and GC-MS.
A mixture of arabinoxylan oligosaccharides from wheat seedling was permethylated and analyzed by electrospray ion trap MS and GC-MS. The presence of isomeric structures differing in degree of branching and position of the branched residue along the xylose backbone was demonstrated for oligosaccharides with four and five monosaccharide residues. No isomeric structures were found for oligosaccharides with three monosaccharide residues. Linkage analysis by GC-MS reveals that xylose residues were substituted with single arabinoxyl residues at C-3.
Matamoros Fernández
Lobvi E
LE
Department of Biochemistry and Molecular Biology, Institute of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark. lobvi@yahoo.com
Obel
Nicolai
N
Scheller
Henrik Vibe
HV
Roepstorff
Peter
P
D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007536:Isomerism; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014990:Xylans / Q000737:chemistry
D009844:Oligosaccharides; D010936:Plant Extracts; D014990:Xylans; C085118:arabinoxylan