Neuronal protein NP25 interacts with F-actin.
Neuronal protein NP25 is a neuron-specific protein present in highly differentiated neural cells, but its functional properties have not been well characterized. NP25 shows high amino acid sequence homology with the smooth muscle cell cytoskeleton-associated proteins, SM22, mp20, and calponin. To gain an insight into the biological functions of NP25, we first examined its subcellular localization in the human neuroblastoma cell line, SK-N-SH. NP25 diffusely distributed in the cytoplasm and fiber-like staining was also observed. It showed that NP25 co-localized with F-actin on stress fibers. A co-sedimentation assay demonstrated that NP25 bound to filamentous actin. Further investigations using fluorescence resonance energy transfer (FRET) technique revealed intracellular binding of NP25 and actin. The significance of the interaction between NP25 and F-actin is discussed.
Mori
Kenji
K
Department of Neurosurgery, Gifu University School of Medicine, Tsukasa-machi, Gifu, Japan.
Muto
Yoshinori
Y
Kokuzawa
Jouji
J
Yoshioka
Takashi
T
Yoshimura
Shinichi
S
Iwama
Toru
T
Okano
Yukio
Y
Sakai
Noboru
N
D000199:Actins / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D003599:Cytoskeleton / Q000378:metabolism; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism
D000199:Actins; D009419:Nerve Tissue Proteins; C422902:TAGLN3 protein, human