title: PCR-DGGE differentiation of strains of Saccharomyces sensu stricto.
abstract: A quick molecular biology method based on the polymerase chain reaction (PCR) and Denaturing Gradient Gel Electrophoresis (DGGE) was developed for distinguishing strains belonging to the Saccharomyces sensu stricto group. Differentiation was obtained between S. cerevisiae, S. paradoxus and S. bayanus / S. pastorianus although no distinction was possible between S. bayanus and S. pastorianus using the amplification of the ITS regions. The ability to distinguish between different strains of the Saccharomyces sensu stricto group could allow for a better understanding of the ecology of these species on grapes as well as in musts and wines and the method developed can be useful for the quick identification of Saccharomyces sensu stricto strains from numerous isolates.
authors.affiliation: Dipartimento di Scienze degli Alimenti, Facoltà di Agraria, University of Udine, via Marangoni 97, 33100 Udine, Italy. Marisa.Manzano@DSA.uniud.it
authors.forename: Marisa
authors.initials: M
authors.lastname: Manzano
authors.forename: Luca
authors.initials: L
authors.lastname: Cocolin
authors.forename: Benedetta
authors.initials: B
authors.lastname: Longo
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Comi
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D012440:Saccharomyces / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D017931:DNA Primers