title: The prevalence of the HPV 16 genome, integrated viral status and p53 genotype in cervical cancer population of north-eastern Hungary, the correlation with the established markers of tumour progression.
abstract: OBJECTIVE
  To evaluate the prevalence of the HPV 16 integrated status and the p53 genotype in cervical cancer in north-eastern Hungary and their correlation with the established prognostic factors.
  
  
  METHODS
  Parallel with the routine histological examination, Southern blot hybridisation and multiplex PCRs were used to detect type/physical state of HPV DNA in primary tumours and in regional lymph nodes combined with p53 genotyping of 83 patients.
  
  
  RESULTS
  46.9% (39/83) prevalence rate of HPV 16 genome was found. The frequency of viral integration (76.9% in primary tumours and 95.2% in regional lymph nodes) and that of the p53Arg homozygous genotype (64.1%) proved to be higher than reported from other parts of the world. The HPV 16 integration and the p53 genotype, failed to correlate with the FIGO stage and lymphatic spread.
  
  
  CONCLUSIONS
  The prevalence of the integrated status of the HPV 16 genome combined with homozygous p53Arg genotype is relatively high in Hungary. These factors however failed to show a strong correlation with the established markers of tumour progression.
authors.affiliation: Department of Gynaecologic Oncology, University of Debrecen, Medical and Health Science Center, H-4012 Debrecen, P.O. Box 37, Hungary. hz@jaguar.dote.hu
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Hernádi
authors.forename: Tamás
authors.initials: T
authors.lastname: Sápy
authors.forename: Zoárd T
authors.initials: ZT
authors.lastname: Krasznai
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis; D018450:Disease Progression; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D005838:Genotype; D006801:Humans; D006814:Hungary / Q000453:epidemiology; D008875:Middle Aged; D009367:Neoplasm Staging; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*; D015995:Prevalence; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology* / Q000235:genetics / Q000473:pathology / Q000821:virology
chemical_list: D014408:Biomarkers, Tumor; D004279:DNA, Viral