Control of bacteria adhesion by cell-wall engineering.
UDP-MurNAc-pentapeptide derivative bacterial cell-wall precursors were synthesized as effective tools for surface display on living bacteria. Lactobacilli were incubated in the ketone-modified precursor-containing medium, and the ketone moiety was displayed on the bacterial surface through cell-wall biosynthesis. Oligomannose was coupled with the ketone moiety on the bacterial surface via a aminooxyl linker, thereby displaying this oligosaccharide on the surface of the bacteria. The increase in the adhesion of the sugar-displaying bacteria onto a concanavalin A-attached film compared to that of native bacteria was confirmed by microscopic observation and surface plasmon resonance measurement. The incorporation of the artificial cell-wall precursors was enhanced when incubated with fosfomycin, an inhibitor of cell-wall precursor biosynthesis.
Sadamoto
Reiko
R
Shionogi Laboratory of Biomolecular Chemistry, Hokkaido University, Kita 21 Nishi 8, Kita-ku, Sapporo 001-0021, Japan.
Niikura
Kenichi
K
Ueda
Taichi
T
Monde
Kenji
K
Fukuhara
Norio
N
Nishimura
Shin-Ichiro
S
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001422:Bacterial Adhesion; D002473:Cell Wall / Q000378:metabolism*; D003208:Concanavalin A / Q000494:pharmacology; D005578:Fosfomycin / Q000494:pharmacology; D007778:Lactobacillus / Q000378:metabolism*; D015394:Molecular Structure; D009844:Oligosaccharides / Q000096:biosynthesis*; D020349:Surface Plasmon Resonance; D014538:Uridine Diphosphate N-Acetylmuramic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D009844:Oligosaccharides; D014538:Uridine Diphosphate N-Acetylmuramic Acid; D003208:Concanavalin A; C002225:UDP-N-acetylmuramic acid pentapeptide; D005578:Fosfomycin