title: Membrane binding and translocation of cell-penetrating peptides.
abstract: Cell-penetrating peptides (CPPs) have been extensively studied during the past decade, because of their ability to promote the cellular uptake of various cargo molecules, e.g., oligonucleotides and proteins. In a recent study of the uptake of several analogues of penetratin, Tat(48-60) and oligoarginine in live (unfixed) cells [Thorén et al. (2003) Biochem. Biophys. Res. Commun. 307, 100-107], it was found that both endocytotic and nonendocytotic uptake pathways are involved in the internalization of these CPPs. In the present study, the membrane interactions of some of these novel peptides, all containing a tryptophan residue to facilitate spectroscopic studies, are investigated. The peptides exhibit a strong affinity for large unilamellar vesicles (LUVs) containing zwitterionic and anionic lipids, with binding constants decreasing in the order penetratin > R(7)W > TatP59W > TatLysP59W. Quenching studies using the aqueous quencher acrylamide and brominated lipids indicate that the tryptophan residues of the peptides are buried to a similar extent into the membrane, with an average insertion depth of approximately 10-11 A from the bilayer center. The membrane topology of the peptides was investigated using an assay based on resonance energy transfer between tryptophan and a fluorescently labeled lysophospholipid, lysoMC, distributed asymmetrically in the membranes of LUVs. By determination of the energy transfer efficiency when peptide was added to vesicles with lysoMC present exclusively in the inner leaflet, it was shown that none of the peptides investigated is able to translocate across the lipid membranes of LUVs. By contrast, confocal laser scanning microscopy studies on carboxyfluorescein-labeled peptides showed that all of the peptides rapidly traverse the membranes of giant unilamellar vesicles (GUVs). The choice of model system is thus crucial for the conclusions about the ability of CPPs to translocate across lipid membranes. Under the conditions used in the present study, peptide-lipid interactions alone cannot explain the different cellular uptake characteristics exhibited by these peptides.
authors.affiliation: Department of Chemistry and Bioscience, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden. thoren@phc.chalmers.se
authors.forename: Per E G
authors.initials: PE
authors.lastname: Thorén
authors.forename: Daniel
authors.initials: D
authors.lastname: Persson
authors.forename: Elin K
authors.initials: EK
authors.lastname: Esbjörner
authors.forename: Mattias
authors.initials: M
authors.lastname: Goksör
authors.forename: Per
authors.initials: P
authors.lastname: Lincoln
authors.forename: Bengt
authors.initials: B
authors.lastname: Nordén
mesh_terms: D020106:Acrylamide / Q000737:chemistry; D000595:Amino Acid Sequence; D001966:Bromine / Q000737:chemistry; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism; D002463:Cell Membrane Permeability; D057846:Cell-Penetrating Peptides; D003374:Coumarins / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D015696:Gene Products, tat / Q000737:chemistry / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry; D008246:Lysophospholipids / Q000737:chemistry; D021962:Membrane Microdomains / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D021381:Protein Transport; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000737:chemistry
chemical_list: D002352:Carrier Proteins; D057846:Cell-Penetrating Peptides; D003374:Coumarins; D015696:Gene Products, tat; D008051:Lipid Bilayers; D008246:Lysophospholipids; D009841:Oligonucleotides; D010455:Peptides; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; C403208:lysoMC; D020106:Acrylamide; C051388:1,2-dioleoyl-sn-glycero-3-phosphoglycerol; D014364:Tryptophan; C414409:penetratin; C017251:1,2-oleoylphosphatidylcholine; D001966:Bromine