title: Spherocyte shape transformation and release of tubular nanovesicles in human erythrocytes.
abstract: We have studied dodecylmaltoside-induced echinocyte-spheroechincyte-spherocyte shape transformation and membrane vesiculation using transmission electron microscopy (TEM) on freeze-fracture replicas. It is indicated that spherical erythrocyte shape at higher dodecylmaltoside concentration is formed due to loss of membrane in the process where small, mostly tubular nanovesicles are released predominantly from the top of echinocyte and spheroechinocyte spicules.
authors.affiliation: Laboratory of Applied Physics, Faculty of Electrical Engineering, University of Ljubljana, Trzaska 25, SI-1000 Ljubljana, Slovenia. slrd.iglic@fe.uni-lj.si
authors.forename: Ales
authors.initials: A
authors.lastname: Iglic
authors.forename: Peter
authors.initials: P
authors.lastname: Veranic
authors.forename: Kristijan
authors.initials: K
authors.lastname: Jezernik
authors.forename: Miha
authors.initials: M
authors.lastname: Fosnaric
authors.forename: Boris
authors.initials: B
authors.lastname: Kamin
authors.forename: Henry
authors.initials: H
authors.lastname: Hägerstrand
authors.forename: Veronika
authors.initials: V
authors.lastname: Kralj-Iglic
mesh_terms: D048430:Cell Shape / Q000187:drug effects; D022081:Cell Surface Extensions / Q000187:drug effects; D003902:Detergents / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D005614:Freeze Fracturing; D005960:Glucosides / Q000494:pharmacology; D006801:Humans; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D013102:Spherocytes / Q000187:drug effects / Q000473:pathology*
chemical_list: D003902:Detergents; D005960:Glucosides; C040358:dodecyl maltoside