title: Estradiol regulation of oviductin/oviduct-specific glycoprotein messenger ribonucleic acid expression in human oviduct mucosal cells in vitro.
abstract: OBJECTIVE
  To determine whether oviduct mucosal cell culture with exogenous 17beta E(2) supports the continued production of oviductin, a putative embryotrophic protein.
  
  
  METHODS
  Semiquantitative reverse-transcriptase polymerase chain reaction analysis of oviductin mRNA expression after oviduct mucosal cell culture in the presence of 17beta E(2). Three different culture systems were studied to investigate the response to E(2).
  
  
  METHODS
  University-based obstetrics and gynecology department.
  
  
  METHODS
  Oviduct tissue was obtained from 18 women undergoing laparoscopy for benign gynecologic conditions.
  
  
  METHODS
  The mucosal layer was isolated from the oviduct tissue and exposed to three different culture systems, which contained various concentrations of 17beta E(2), or vehicle-only control.
  
  
  METHODS
  The relationship between exposure to 17beta E(2) and expression of oviductin messenger (m)RNA by cultured oviduct mucosal cells.
  
  
  RESULTS
  There was a significant increase in oviductin mRNA expression after the addition of 17beta E(2) to the culture system in which the in vivo cell-to-cell and cell-to-basement-membrane contacts of the oviduct had been maintained.
  
  
  CONCLUSIONS
  Estradiol failed to alter oviductin mRNA expression in oviduct mucosal cells cultured under conditions in which the ciliated mucosal cell phenotype plus the cell-to-cell and cell-to-basement-membrane contacts of the oviduct were lost. However, with a culture system that maintained the cell architecture, E(2) initiated and significantly increased oviductin mRNA expression.
authors.affiliation: Department of Obstetrics and Gynecology, The Chinese University of Hong Kong, Hong Kong, SAR, China. CBritonJones@aol.com
authors.forename: Christine
authors.initials: C
authors.lastname: Briton-Jones
authors.forename: Ingrid Hung
authors.initials: IH
authors.lastname: Lok
authors.forename: Che Kwok
authors.initials: CK
authors.lastname: Cheung
authors.forename: Tony Tak Yu
authors.initials: TT
authors.lastname: Chiu
authors.forename: Lai Ping
authors.initials: LP
authors.lastname: Cheung
authors.forename: Christopher
authors.initials: C
authors.lastname: Haines
mesh_terms: D002478:Cells, Cultured; D003584:Cytological Techniques; D004958:Estradiol / Q000494:pharmacology*; D005187:Fallopian Tubes / Q000166:cytology / Q000378:metabolism*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D009092:Mucous Membrane / Q000166:cytology / Q000378:metabolism; D009924:Organ Culture Techniques; D012333:RNA, Messenger / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology
chemical_list: D006023:Glycoproteins; C091468:OVGP1 protein, human; D012333:RNA, Messenger; D004958:Estradiol