title: Trypanosoma cruzi isolates from Chile are heterogeneous and composed of mixed populations when characterized by schizodeme and Southern analyses.
abstract: In total, 61 Chilean isolates of Trypanosoma cruzi, were analysed using schizodeme and Southern analysis, using as probes the highly variable regions of minicircles from cloned parasites. Isolates were collected and amplified from domestic and wild triatomines, and from infected subjects in all the endemic areas of Chile. Three major parasite genotypes could be detected in the domestic transmission cycle, whilst 1 major T. cruzi genotype is circulating in the wild transmission cycle. Schizodeme analysis suggested that T. cruzi isolates are mixed populations, whereas the Southern analyses detected only 3 mixed isolates using 4 selected minicircle segments as probes.
authors.affiliation: Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Casilla 70086, Santiago 7, Chile.
authors.forename: J P
authors.initials: JP
authors.lastname: Torres
authors.forename: S
authors.initials: S
authors.lastname: Ortiz
authors.forename: S
authors.initials: S
authors.lastname: Muñoz
authors.forename: A
authors.initials: A
authors.lastname: Solari
mesh_terms: D000818:Animals; D000940:Antigenic Variation / Q000235:genetics; D015139:Blotting, Southern; D002677:Chile; D018105:DNA, Kinetoplast / Q000737:chemistry / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D006801:Humans; D008297:Male; D051379:Mice; D009693:Nucleic Acid Hybridization; D014224:Triatoma / Q000469:parasitology; D014349:Trypanosoma cruzi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014352:Trypanosomiasis / Q000469:parasitology*
chemical_list: D018105:DNA, Kinetoplast; D016054:DNA, Protozoan