Systemic delivery of synthetic siRNAs.
The ability to regulate endogenous gene expression by cleaving mRNA is important in basic and applied biological research. This is now driven predominantly by small interfering RNAs (siRNAs) as they induce sequence-specific gene silencing. However, the major obstacle to the use of siRNAs as therapeutics is the difficulty involved in effective in vivo delivery. This chapter describes the liposomal delivery of siRNAs into adult mice.
Sioud
Mouldy
M
Department of Immunology, Molecular Medicine Group, The Norwegian Radium Hospital, Oslo.
Sørensen
Dag R
DR
D000818:Animals; D001692:Biological Transport; D020868:Gene Silencing; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D010533:Peritoneal Lavage / Q000379:methods; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D014162:Transfection
D008081:Liposomes; D034741:RNA, Small Interfering