title: Inhibition of the development of hepatocellular carcinomas by phenyl N-tert-butyl nitrone in rats fed with a choline-deficient, L-amino acid-defined diet.
abstract: Effects of phenyl N-tert-butyl nitrone (PBN), a spin-trapping agent, on the development of frank cancers were examined in male Wistar rats fed with a choline-deficient, l-amino acid-defined (CDAA) diet for 70 weeks. PBN (0.065% in the drinking water) reduced incidences, multiplicities and possibly sizes of both hepatocellular adenomas and carcinomas when administered for all 70 weeks or only for the first 26 weeks, and those of carcinomas but not adenomas, when administered only for the last 44 weeks. These results indicate that PBN can prevent the development of frank HCCs in the CDAA diet model. The anti-carcinogenic effect of PBN may be ascribed to the prevention of both the development of HCAs and their malignant conversion to HCCs. If such findings can be generalized, PBN may be able to serve as a good tool to investigate molecular mechanisms underlying carcinogenic processes.
authors.affiliation: Department of Pathology, Sasaki Institute, Sasaki Foundation, 2-2 Kanda-Surugadai, Chiyoda, Tokyo 101-0062, Japan. dai.nakae@sasaki.or.jp
authors.forename: Dai
authors.initials: D
authors.lastname: Nakae
authors.forename: Fumiyuki
authors.initials: F
authors.lastname: Uematsu
authors.forename: Hideki
authors.initials: H
authors.lastname: Kishida
authors.forename: Osamu
authors.initials: O
authors.lastname: Kusuoka
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Katsuda
authors.forename: Midori
authors.initials: M
authors.lastname: Yoshida
authors.forename: Masakazu
authors.initials: M
authors.lastname: Takahashi
authors.forename: Akihiko
authors.initials: A
authors.lastname: Maekawa
authors.forename: Ayumi
authors.initials: A
authors.lastname: Denda
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Konishi
authors.forename: Yashige
authors.initials: Y
authors.lastname: Kotake
authors.forename: Robert A
authors.initials: RA
authors.lastname: Floyd
mesh_terms: D000236:Adenoma / Q000209:etiology / Q000517:prevention & control*; D000596:Amino Acids / Q000008:administration & dosage*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000209:etiology / Q000517:prevention & control*; D002796:Choline Deficiency / Q000150:complications*; D003497:Cyclic N-Oxides; D004032:Diet; D008114:Liver Neoplasms, Experimental / Q000209:etiology / Q000517:prevention & control*; D008297:Male; D009589:Nitrogen Oxides / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D014867:Water
chemical_list: D000596:Amino Acids; D003497:Cyclic N-Oxides; D009589:Nitrogen Oxides; D014867:Water; C029217:phenyl-N-tert-butylnitrone