The immobilization of hepatocytes on 24 nm-sized gold colloid for enhanced hepatocytes proliferation.
Bioartificial liver and hepatocyte transplantation is anticipated to supply a temporary metabolic support for candidates of liver transplantation or for patients with fulminant liver failure. An essential restriction of this form is the inability to acquire an enough amount of hepatocytes. Enhancement of the proliferation and differentiated function of hepatocytes is becoming a pursued target. Here, porcine hepatocytes were successfully immobilized on nano-sized gold colloid particles to construct a "hepatocyte/gold colloid" interface at which hepatocytes can be quickly proliferated. The properties of this resulting interface were characterized and confirmed by scanning electron microscopy and atomic force microscopy. The proliferative mechanism of hepatocytes was also discussed. The proliferated hepatocytes could be applied to the clinic based on their excellent functions for the synthesis of protein, glucose and urea as well as lower lactate dehydrogenase release.
Gu
Hai-Ying
HY
Department of Chemistry, State Key Laboratory of Coordination Chemistry, Institute of Analytical Science, Nanjing University, Nanjing 210093, China.
Chen
Zhong
Z
Sa
Rong-Xiao
RX
Yuan
Su-Su
SS
Chen
Hong-Yuan
HY
Ding
Yi-Tao
YT
Yu
Ai-Min
AM
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division / Q000502:physiology; D018914:Cells, Immobilized / Q000502:physiology; D005260:Female; D017979:Gold Colloid / Q000737:chemistry*; D022781:Hepatocytes / Q000166:cytology* / Q000502:physiology*; D008297:Male; D008422:Materials Testing; D036103:Nanotechnology / Q000379:methods; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure*; D013499:Surface Properties; D013552:Swine; D013556:Swine, Miniature; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D017979:Gold Colloid