Peptides from anchovy sauce induce apoptosis in a human lymphoma cell (U937) through the increase of caspase-3 and -8 activities.
Various biological activities of peptides have been found. In this study, the induction of apoptosis in a human lymphoma cell line (U937) by peptide fraction separated from anchovy sauce was studied using biochemical and flow cytometric methods. After U937 was treated with a hydrophobic peptide fraction of the anchovy sauce, a sub-G1 peak, which represents apoptosis, was found in the cell cycle analysis. The apoptosis in the peptide-treated U937 cell was also confirmed by apoptotic DNA fragmentation. Furthermore, an increase of caspase-3 and -8 activities was observed. Our results suggest that the peptide fraction separated from the anchovy sauce may have cancer chemopreventive effects through the induction of apoptosis in cancer cells.
Lee
Young Gon
YG
Department of Food Science and Technology and School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, South Korea.
Kim
Ji Yeon
JY
Lee
Ki Won
KW
Kim
Kyoung Heon
KH
Lee
Hyong Joo
HJ
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D053181:Caspase 8; D020169:Caspases / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D005399:Fishes; D005511:Food Handling; D006801:Humans; D007700:Kinetics; D010455:Peptides / Q000302:isolation & purification / Q000633:toxicity*; D020298:U937 Cells
D010455:Peptides; C505407:CASP3 protein, human; C505530:CASP8 protein, human; D053148:Caspase 3; D053181:Caspase 8; D020169:Caspases