Spherocyte shape transformation and release of tubular nanovesicles in human erythrocytes.
We have studied dodecylmaltoside-induced echinocyte-spheroechincyte-spherocyte shape transformation and membrane vesiculation using transmission electron microscopy (TEM) on freeze-fracture replicas. It is indicated that spherical erythrocyte shape at higher dodecylmaltoside concentration is formed due to loss of membrane in the process where small, mostly tubular nanovesicles are released predominantly from the top of echinocyte and spheroechinocyte spicules.
Iglic
Ales
A
Laboratory of Applied Physics, Faculty of Electrical Engineering, University of Ljubljana, Trzaska 25, SI-1000 Ljubljana, Slovenia. slrd.iglic@fe.uni-lj.si
Veranic
Peter
P
Jezernik
Kristijan
K
Fosnaric
Miha
M
Kamin
Boris
B
Hägerstrand
Henry
H
Kralj-Iglic
Veronika
V
D048430:Cell Shape / Q000187:drug effects; D022081:Cell Surface Extensions / Q000187:drug effects; D003902:Detergents / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D005614:Freeze Fracturing; D005960:Glucosides / Q000494:pharmacology; D006801:Humans; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D013102:Spherocytes / Q000187:drug effects / Q000473:pathology*
D003902:Detergents; D005960:Glucosides; C040358:dodecyl maltoside