title: Mitotic instability associated with late genomic changes in bone and soft tissue tumours.
abstract: One source of genomic instability in tumours is abnormal mitotic segregation of chromosomes. Evaluation of chromosome segregation and cytogenetic aberrations in 28 bone and soft tissue neoplasms revealed few mitotic disturbances in benign lesions, whereas most of the malignant tumours, except for chondrosarcomas, showed anaphase bridges and/or multipolar mitoses. Only cases with chromosomal imbalances exhibited these defects and they were not present in any of the cases with sole primary changes, indicating that mitotic instability is established relatively late in mesenchymal tumour development. Most cases with multipolar mitoses exhibited abnormal centrosome configurations. However, induction of supernumerary centrioles in vitro failed to produce mitotic abnormalities in normal cells, indicating that additional disturbances of the cell division machinery are required for the generation of mitotic multipolarity.
authors.affiliation: Department of Clinical Genetics, University Hospital, Lund SE-221 85, Sweden. david.gisselsson@klingen.lu.se
authors.forename: David
authors.initials: D
authors.lastname: Gisselsson
authors.forename: Eva
authors.initials: E
authors.lastname: Pålsson
authors.forename: Christina
authors.initials: C
authors.lastname: Yu
authors.forename: Fredrik
authors.initials: F
authors.lastname: Mertens
authors.forename: Nils
authors.initials: N
authors.lastname: Mandahl
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001859:Bone Neoplasms / Q000235:genetics*; D018385:Centrosome; D002869:Chromosome Aberrations; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D042822:Genomic Instability; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D008938:Mitosis / Q000235:genetics*; D011003:Ploidies; D012983:Soft Tissue Neoplasms / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm