Diversity of Cryptococcus and Dioszegia yeasts (Basidiomycota) inhabiting arbuscular mycorrhizal roots or spores.
The genera Cryptococcus and Dioszegia contain basidiomycetous yeasts found in a wide range of habitats. Primers to amplify the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA (nrDNA) of arbuscular mycorrhizal fungi (AMF) also allow detecting members of this yeast group. Here we report the results of a sequence analysis using maximum parsimony on a set of 50 ITS sequences of yeasts associated with AMF structures (roots of 26 plant species, AM spores) from six field sites in Central Germany. Among 10 separated taxa, respectively five in the Tremellales and two in the Filobasidiales had unknown sequences. Therefore it was not possible to assign these sequences to any known species. The study indicates that exploring the diversity of Cryptococcus and Dioszegia in soil habitats with molecular methods might enlarge the actually estimated biodiversity of the group.
Renker
Carsten
C
Terrestrial Ecology, Institute of Botany, University of Leipzig, Johannisallee 21, D-04103 Leipzig, Germany. crenker@uni-leipzig.de
Blanke
Verena
V
Börstler
Boris
B
Heinrichs
Jochen
J
Buscot
François
F
D001487:Basidiomycota / Q000145:classification* / Q000235:genetics; D003454:Cryptococcus / Q000145:classification* / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D017753:Ecosystem; D008969:Molecular Sequence Data; D038821:Mycorrhizae; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013172:Spores, Fungal / Q000502:physiology*
D021903:DNA, Ribosomal Spacer