The investigation of cytosolic phospholipase A2 using ELISA.
Two studies of the behaviour of cytosolic phospholipase A(2) (cPLA(2)) in the red blood cell (RBC), as measured by ELISA, are described. In the first study we show a significant increase in cPLA(2) in patients with schizophrenia compared to controls and suggest that this measure, if corroborated, could be used as a diagnostic marker. In a second study we found that washing the RBC introduced an unknown confounding variable which led us to reject this study. A subsequent investigation of washing red cells showed that the washing effect may be due to a plasma factor likely to be more than 5kDa MW which can be removed from red cells by washing with buffers. When the cells are washed, the concentration of cPLA(2) in the red cell, as measured by ELISA, significantly increases. We advise against washing the red cell in any study that involves measuring cPLA(2) by ELISA.
Macdonald
Donald J
DJ
Department of Biochemistry, Victoria Infirmary, Glasgow G42 9TY, Scotland, UK. glasgow.pla2group@gvic.scot.nhs.uk
Boyle
Rose M
RM
Glen
Alastair C A
AC
Ross
Brian M
BM
Glen
A Iain M
AI
Ward
Pauline E
PE
McKinney
Siofradh B
SB
Peterkin
Myrtle A
MA
D016477:Artifacts; D002021:Buffers; D016022:Case-Control Studies; D003600:Cytosol / Q000201:enzymology*; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes; D006801:Humans; D010741:Phospholipases A / Q000097:blood / Q000378:metabolism*; D054467:Phospholipases A2; D012559:Schizophrenia / Q000097:blood / Q000201:enzymology*
D002021:Buffers; D010741:Phospholipases A; D054467:Phospholipases A2