title: Development of a gas chromatographic test for the quantification of the biomarker 3-bromopropionic acid in human urine.
abstract: An accurate and precise method was developed for the detection and quantification of 3-bromopropionic acid (3-BPA), a metabolite and biomarker for exposure to 1-bromopropane (1-BP). 1-BP is used as an industrial solvent and exposure is a health concern for industrial workers due to its toxicity. It has been associated with neurological disorders in both animals and humans. Urine sample preparation for the determination of 3-BPA consisted of liquid-liquid extraction (LLE) with ethyl acetate and silylation with N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide (MTBSTFA). Quantification was by means of a gas chromatograph (GC) equipped with a mass selective detector (MSD) using a dimethylpolysiloxane (HP-1) capillary column and 3-chloropropionic acid was used as an internal standard in the procedure. Demonstrated accuracy and precision during this method's validation was good; recovery varied between 93 and 98% with relative standard deviations (R.D.S.) of 5.7% or less. The limit of detection (LOD) for the procedure was approximately 0.01microg/ml 3-BPA in urine. These data and other factors of the development and validation of this test method will be discussed.
authors.affiliation: U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Division of Applied Research and Technology, Taft Laboratory C-26, Cincinnati, OH 45226, USA. cbhymer@cdc.gov
authors.forename: C
authors.initials: C
authors.lastname: B'Hymer
authors.forename: K L
authors.initials: KL
authors.lastname: Cheever
mesh_terms: D015415:Biomarkers / Q000652:urine*; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D011422:Propionates / Q000652:urine*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D011422:Propionates; C035776:3-bromopropionic acid