Intermediate fertile Triticum aestivum (+) Agropyron elongatum somatic hybrids are generated by low doses of UV irradiation.
We report the production and characterization of somatic hybrids between Triticum aestivum L. and Agropyron elongatum (Host) Nevishi (the synonym is Thinopyrum ponticum). Asymmetric protoplast fusion was performed between Agropyron elongatum protoplasts irradiated with a low UV dose and protoplasts of wheat taken from nonregenerable suspension cultures. More than 40 green plantlets were obtained from 15 regenerated clones and one of them produced seeds. The phenotypes of the hybrid plants and seeds were intermediate between wheat and Agropyron elongatum. All of the regenerated calli and plants were verified as intergeneric hybrids on the basis of morphological observation and analysis of isozyme, cytological, 5SrDNA spacer sequences and random amplified polymorphic DNA (RAPD). RFLP analysis of the mitochondrial genome revealed evidence of random segregation and recombination of mtDNA.
Cheng
Ai Xia
AX
School of Life Sciences, Shandong University, Jinan 250100, Shandong, China.
Xia
Guang Min
GM
Zhi
Da Ying
DY
Chen
Hui Min
HM
D031702:Agropyron / Q000235:genetics* / Q000528:radiation effects; D015139:Blotting, Southern; D002459:Cell Fusion; D032461:Chromosomes, Plant / Q000737:chemistry / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D004307:Dose-Response Relationship, Radiation; D004591:Electrophoresis, Polyacrylamide Gel; D004950:Esterases / Q000032:analysis; D005298:Fertility / Q000235:genetics / Q000528:radiation effects; D005838:Genotype; D006822:Hybrid Cells / Q000737:chemistry / Q000166:cytology / Q000201:enzymology; D006824:Hybridization, Genetic / Q000528:radiation effects*; D007527:Isoenzymes / Q000032:analysis; D009195:Peroxidase / Q000032:analysis; D010641:Phenotype; D063245:Plant Development; D010944:Plants / Q000033:anatomy & histology / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011523:Protoplasts / Q000166:cytology / Q000528:radiation effects; D019105:Random Amplified Polymorphic DNA Technique; D011995:Recombination, Genetic / Q000235:genetics; D012639:Seeds / Q000033:anatomy & histology; D014908:Triticum / Q000235:genetics* / Q000528:radiation effects; D014466:Ultraviolet Rays
D004272:DNA, Mitochondrial; D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer; D007527:Isoenzymes; D009195:Peroxidase; D004950:Esterases