Determination of abamectin and doramectin in sheep faeces using HPLC with fluorescence detection.
An analytical procedure for the determination of abamectin and/or doramectin in sheep faeces has been developed. Avermectins were extracted from sheep faeces with acetonitrile, clean-up using solid phase extraction (SPE) and analysed by high performance liquid chromatography (HPLC) with fl uorescence detection after derivatization with N-methylimidazole.The method has a low detection limit (1.0 ng/g of moist sheep faeces), low quanti fi cation limit (2.5 ng/g of moist sheep faeces), good recovery in the range 66.4-80.8% for abamectin and 67.7-85.5% for doramectin as well as good repeatability (>85%). The method is applicable to the study of the time pro fi le of excretion in sheep faeces and also for ecotoxicological studies of both avermectins.
Kolar
Lucija
L
University of Ljubljana, Veterinary Faculty, Institute of Physiology, Pharmacology and Toxicology, Gerbiceva 60, 1000 Ljubljana, Slovenia. lucija.kolar@vf.uni-lj.si
Kuzner
Jernej
J
Erzen
Nevenka Kozuh
NK
D000818:Animals; D000871:Anthelmintics / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005243:Feces / Q000737:chemistry*; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis*; D012680:Sensitivity and Specificity; D012756:Sheep; D013050:Spectrometry, Fluorescence / Q000379:methods*
D000871:Anthelmintics; C048324:abamectin; D007559:Ivermectin; C084101:doramectin