[Studies on organ phytomorphology of leaves from Rehmannia glutinosa].
OBJECTIVE
To set up the fittest medium and optimum condition for leaves from Rehmannia glutinosa to form regenerated plantlets directly and to induce microtubers.


METHODS
Leaves of different phases obtained from the virus-free plantlets were incubated in some kinds of media on the back side.


CONCLUSIONS
The fittest medium for the formation of regenerated plantlet was MS + 6-BA 2.0 mg.L-1 and the fittest medium for the induction of microtuber was MS + 6-BA 1.0 mg.L-1 + NAA 0.5 mg.L-1. The optimum incubation temperaue was 25 degrees C and the light length was (2,000-3,000 lx) 12 h.d-1.
Xue
Jian-ping
JP
Department of Biology, Huaibei Coal Industry Teacher's College, Huabei 235000, Anhui, China. xuejp2000@yahoo.com.cn
Wang
Ru-yi
RY
Zhang
Ai-min
AM
Liu
Jun
J
D003470:Culture Media; D046508:Culture Techniques; D008029:Lighting; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000254:growth & development / Q000502:physiology; D010946:Plants, Medicinal / Q000254:growth & development* / Q000502:physiology; D012038:Regeneration; D032267:Rehmannia / Q000254:growth & development* / Q000502:physiology; D013696:Temperature
D003470:Culture Media; D010937:Plant Growth Regulators