title: The central region of human immunodeficiency virus type 1 p6 protein (Gag residues S14-I31) is dispensable for the virus in vitro.
abstract: The human immunodeficiency virus type 1 p6 region encodes p6(Gag) and the transframe p6(Pol) protein. The Gag frame encodes an N-terminal late assembly L domain and a C-terminal Vpr binding domain. In the Pol frame, substitution at a C-terminal motif decreases protease autocleavage. The role of the highly polymorphic central region of p6, comprising amino acids S14-I31 (p6(Gag)) and R20-D39 (p6(Pol)), is unclear. Analysis of this central region demonstrated that 35 % of p6(Gag) appears to be dispensable for virus propagation in vitro and smaller deletion and insertion polymorphisms can be tolerated in vivo. Extensive Pol deletion (deltaR20-D39, 42 % of p6(Pol)) did not alter protease autocleavage.
authors.affiliation: Division of Infectious Diseases, University of Lausanne, Lausanne, Switzerland.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Bleiber
authors.affiliation: Division of Infectious Diseases, University of Lausanne, Lausanne, Switzerland.
authors.forename: Solange
authors.initials: S
authors.lastname: Peters
authors.affiliation: Division of Infectious Diseases, University of Lausanne, Lausanne, Switzerland.
authors.forename: Raquel
authors.initials: R
authors.lastname: Martinez
authors.affiliation: University Hospital, and Center of Electron Microscopy, University of Lausanne, Lausanne, Switzerland.
authors.forename: Dusan
authors.initials: D
authors.lastname: Cmarko
authors.affiliation: Institute of Microbiology, University of Lausanne, Lausanne, Switzerland.|Division of Infectious Diseases, University of Lausanne, Lausanne, Switzerland.
authors.forename: Pascal
authors.initials: P
authors.lastname: Meylan
authors.affiliation: Institute of Microbiology, University of Lausanne, Lausanne, Switzerland.|Division of Infectious Diseases, University of Lausanne, Lausanne, Switzerland.
authors.forename: Amalio
authors.initials: A
authors.lastname: Telenti
mesh_terms: D000595:Amino Acid Sequence; D015683:Gene Products, gag / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D015684:Gene Products, pol / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D015658:HIV Infections / Q000821:virology; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D010641:Phenotype; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic; D017384:Sequence Deletion; D014774:Virulence / Q000235:genetics / Q000502:physiology; D014779:Virus Replication / Q000235:genetics / Q000502:physiology
chemical_list: D015683:Gene Products, gag; D015684:Gene Products, pol