Participation of transcription factors from the Rel/NF-kappa B family in the circadian system in hamsters.
We have studied the presence and activity of components of the nuclear factor-kappaB (NF-kappaB) transcription factor in the hamster circadian system analyzing wheel-running activity, protein expression and DNA binding activity by electrophoresis mobility shift assays (EMSA). Non-rhythmic specific immunoreactive bands corresponding to a NF-kappaB subunit (p65) were found in hamster suprachiasmatic nuclei (SCN) homogenates. The active form of NF-kappaB evidenced by EMSA was clear and specific in SCN nuclear extracts. The administration of the NF-kappaB inhibitor pyrrolidine-dithiocharbamate (PDTC) blocked the light-induced phase advance at circadian time 18 (vehicle+light pulse: 2.08+/-0.46 h, PDTC+light: 0.36+/-0.35 h). These results demonstrate the presence and activity of Rel/NF-kappaB family proteins in the hamster SCN and suggest that these proteins may be related to the entrainment and regulation of circadian rhythms.
Marpegan
Luciano
L
Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Sáenz Peña 180, Bernal, B1876BXD Pcia. de Buenos Aires, Argentina.
Bekinschtein
Tristan A
TA
Freudenthal
Ramiro
R
Rubio
M Fernanda
MF
Ferreyra
Gabriela A
GA
Romano
A
A
Golombek
D A
DA
D000818:Animals; D002940:Circadian Rhythm / Q000502:physiology*; D006224:Cricetinae; D008297:Male; D008647:Mesocricetus; D016328:NF-kappa B / Q000096:biosynthesis* / Q000235:genetics; D020572:Oncogene Proteins v-rel / Q000096:biosynthesis* / Q000235:genetics; D013493:Suprachiasmatic Nucleus / Q000378:metabolism*; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
D016328:NF-kappa B; D020572:Oncogene Proteins v-rel; D014157:Transcription Factors