Cryopreservation of shoot-tips of citrus using vitrification: effect of reduced form of glutathione.
A commercial citrus scion cultivar, '439' tangor [C. Suavissima Hort. et Tanaka x C. sinensis (L.) Osbesk cv.Gailiangcheng] was used to investigate whether GSH (reduced form of glutathione) could improve survival of a vitrification procedure. The optimal pre-growth treatment was a 3-day pre-culture on basal pre-culture medium (BPM: MT basal medium containing 0.5 mol/L glycerol and 5 % sucrose at pH 5.8). GSH of 40 mg/L in the pre-culture medium improved shoot tip survival after cryopreservation. GSH in the recovery medium also improved survival, with 10 mg/L giving the best result. GSH of 40 mg/L in the loading and vitrification solutions also improved survival. The optimal cryopreservation protocol was successfully applied to 12 other citrus cultivars. This is the first report on the successful cryopreservation of shoot-tips from commercial citrus scion cultivars.
Wang
Zi-Cheng
ZC
National Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China.
Deng
Xiu-Xin
XX
D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002957:Citrus / Q000187:drug effects* / Q000502:physiology; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology*; D018520:Plant Shoots / Q000187:drug effects* / Q000502:physiology
D003451:Cryoprotective Agents; D005978:Glutathione