[Expression of neurotoxin II from Naja oxiana cobra venom in Escherichia coli in a hybrid form with thioredoxin].
Neurotoxin II from the venom of cobra Naja oxiana is a short type alpha-neurotoxin, which competitively inhibits nicotinic acetylcholine receptor. The toxin gene was expressed as a construct fused with the thioredoxin gene and the linker encoding the enteropeptidase recognition site and a Met residue between the genes. The fusion protein was mainly cleaved by cyanogen bromide, since enteropeptidase was less effective. The yield of neurotoxin II was 6 mg/l of the bacterial culture. The resulting recombinant protein was identified with native neurotoxin II by its N-terminal analysis, mass spectrometry, and NMR spectroscopy. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 1; see also http://www.maik.ru.
Liukmanova
E N
EN
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP Moscow, 117997 Russia.
Shul'ga
A A
AA
Arsen'eva
D A
DA
Pluzhnikov
K A
KA
Dolgikh
D A
DA
Arsen'ev
A S
AS
Kirpichnikov
M P
MP
D000818:Animals; D001483:Base Sequence; D003039:Cobra Neurotoxin Proteins / Q000737:chemistry / Q000235:genetics*; D017931:DNA Primers; D004546:Elapid Venoms / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics*; D013058:Mass Spectrometry; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics; D013879:Thioredoxins / Q000235:genetics*
D003039:Cobra Neurotoxin Proteins; D017931:DNA Primers; D004546:Elapid Venoms; D011993:Recombinant Fusion Proteins; D013879:Thioredoxins; C076649:neurotoxin II, Naja naja oxiana