Follicle development following cryopreservation of human ovarian tissue.
Despite the recent increase in human ovarian tissue banking, there has been little progress in establishing whether follicles within this tissue are viable and capable of function following cryopreservation. Two methods to assess growth and developmental potential of cryopreserved tissue are evaluated; (1). isolated follicle culture and (2). xenografting of tissue into a host animal. Development of numerous antral follicles following xenografting of cryopreserved tissue indicates that the cryopreservation procedure can preserve the developmental competence of primordial follicles.
Gook
Debra A
DA
Reproductive Services, Royal Women's Hospital, 132 Grattan St, Carlton, Australia and Melbourne IVF, Melbourne, Vic. 3053, Australia. gookd@cryptic.rch.unimelb.edu.au
Osborn
S M
SM
Archer
J
J
Edgar
D H
DH
McBain
J
J
D000818:Animals; D015925:Cryopreservation; D046508:Culture Techniques; D005260:Female; D006801:Humans; D006080:Ovarian Follicle / Q000254:growth & development*; D014183:Transplantation, Heterologous