Catalpol inhibits apoptosis in hydrogen peroxide-induced PC12 cells by preventing cytochrome c release and inactivating of caspase cascade.
In the present study, using a rat pheochromocytoma (PC12) cell line, the effect of catalpol on H2O2-induced apoptosis was studied. The apoptosis in H2O2-induced PC12 cells was accompanied by down-regulation of Bcl-2, up-regulation of Bax, the release of mitochondrial cytochrome c to cytosol and sequential activation of caspase-1 and caspase-3 then leading to cleavage of poly-ADP-ribose polymerase (PARP). Catalpol not only suppressed the down-regulation of Bcl-2, up-regulation of Bax and the release of mitochondrial cytochrome c to cytosol, but also attenuated caspase-3 activation, PARP cleavage, and eventually protected against H2O2-induced apoptosis. Taken together, these results suggest that treatment of PC12 cells with catalpol can block H2O2-induced apoptosis by the regulation of Bcl-2 family members, as well as suppression of cytochrome c release and caspase cascade activation.
Jiang
B
B
Department of Biochemical Engineering, Dalian University of Technology, Dalian 116024, China. jiangbo0411@sina.com
Liu
J H
JH
Bao
Y M
YM
An
L J
LJ
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D020169:Caspases / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D045304:Cytochromes c / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005786:Gene Expression Regulation; D019254:Genes, bcl-2 / Q000187:drug effects; D005960:Glucosides / Q000494:pharmacology*; D006861:Hydrogen Peroxide; D057888:Iridoid Glucosides; D039823:Iridoids / Q000494:pharmacology*; D016716:PC12 Cells / Q000187:drug effects* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000187:drug effects / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2; D051381:Rats; D051028:bcl-2-Associated X Protein
C494828:Bax protein, rat; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C078040:catalpol; D045304:Cytochromes c; D006861:Hydrogen Peroxide; D020169:Caspases