Identification of a new G-to-A transition mutation at nucleotide position 129 of the Xrcc4 gene in ionizing radiation-hypersensitive mutant LX830 cells.
The mouse lymphoma cell line LX830 is an X-ray-hypersensitive mutant. Complementation tests between LX830 cells and radiation-sensitive mutants of M10 (Xrcc4 deficient cells) or SX10 (DNA ligase IV deficient cells) cells showed that M10 cells did not complement LX830 cells, but SX10 cells did, suggesting that LX830 cells would belong to the X-ray-cross complementation group (XRCC4). A sequence analysis of Xrcc4 cDNA in LX830 cells disclosed a transition of G to A at nucleotide position 129, which resulted in a change of tryptophan (43) to a termination codon. Transfection of the mouse Xrcc4 cDNA rescued the X-ray sensitivity of the mutant cells. LX830 is an Xrcc4-deficient cell line bearing a termination codon in exon 2 of the Xrcc4 gene and no wild-type Xrcc4 gene.
Itsukaichi
Hiromi
H
Radiation Hazards Research Group, National Institute of Radiological Sciences, Chiba, Japan.
Mori
Masahiko
M
Nakamura
Atsuko
A
Sato
Koki
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002459:Cell Fusion; D045744:Cell Line, Tumor; D003062:Codon; D018389:Codon, Nonsense; D004249:DNA Damage; D004260:DNA Repair; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D004307:Dose-Response Relationship, Radiation; D005091:Exons; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D009154:Mutation; D016366:Open Reading Frames; D010957:Plasmids / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011839:Radiation, Ionizing; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014965:X-Rays
D003062:Codon; D018389:Codon, Nonsense; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; C101467:XRCC4 protein, human