Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus.
A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection.
Emery
Shannon L
SL
Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Erdman
Dean D
DD
Bowen
Michael D
MD
Newton
Bruce R
BR
Winchell
Jonas M
JM
Meyer
Richard F
RF
Tong
Suxiang
S
Cook
Byron T
BT
Holloway
Brian P
BP
McCaustland
Karen A
KA
Rota
Paul A
PA
Bankamp
Bettina
B
Lowe
Luis E
LE
Ksiazek
Tom G
TG
Bellini
William J
WJ
Anderson
Larry J
LJ
D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D006801:Humans; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000706:statistics & numerical data; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D045169:Severe Acute Respiratory Syndrome / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology; D014709:Vero Cells
D017931:DNA Primers; D004279:DNA, Viral; D012367:RNA, Viral