title: Serum protein-expression profiling using the ProteinChip biomarker system.
abstract: Protein-expression profiling of serum is a common approach to the discovery of potential diagnostic and therapeutic markers of disease. Like any other proteome, the serum proteome is characterized by protein expression across a large dynamic range. This single facet requires the employment of fractionation procedures prior to detection of protein. The authors use a combination of conventional column chromatography with array-based chromatography to simplify the serum proteome into subproteomes, thus providing a greater representation of the serum proteome. Robotics is employed to increase the throughput of sample processing. These procedures result in large amounts of data that are analyzed through a series of preprocessing and postprocessing steps. A well-designed serum profiling project can therefore result in the discovery of statistically sound, clinically meaningful protein biomarkers.
authors.affiliation: Ciphergen Biosystems, Inc, Fremont, CA, USA.
authors.forename: Kate
authors.initials: K
authors.lastname: Gilbert
authors.forename: Sharel
authors.initials: S
authors.lastname: Figueredo
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Meng
authors.forename: Christine
authors.initials: C
authors.lastname: Yip
authors.forename: Eric T
authors.initials: ET
authors.lastname: Fung
mesh_terms: D000837:Anion Exchange Resins; D001798:Blood Proteins / Q000737:chemistry* / Q000378:metabolism; D002852:Chromatography, Ion Exchange / Q000379:methods; D020869:Gene Expression Profiling; D006801:Humans; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D020543:Proteome / Q000032:analysis*
chemical_list: D000837:Anion Exchange Resins; D001798:Blood Proteins; D020543:Proteome