Improved in vitro development of porcine embryos with different energy substrates and serum.
The effect of replacing 5.5 mM glucose in North Carolina State University (NCSU)-23 medium with 0.5 mM pyruvate/5.0 mM lactate on porcine IVF embryo development was investigated in Experiment 1. Culturing embryos with pyruvate/lactate for 7 days or with pyruvate/lactate from Days 0 to 2, and then glucose from Days 2 to 7 improved cleavage rates. In Experiment 2, embryos were cultured for 7 days in pyruvate/lactate containing NCSU-23 medium supplemented with 0.05% PVA, 0.4% BSA or 10% fetal bovine serum (FBS). The BSA supplement increased the rates of cleavage, blastocyst formation, and the number of total cells in blastocysts. In Experiment 3, embryos were cultured in pyruvate/lactate containing NCSU-23 medium supplemented with 0.4% BSA for 7 days (BSA-PL), 0.4% BSA from Days 0 to 4 and then 10% FBS from Days 4 to 7 (BSA-PL-->F ) or 0.4% BSA from Days 0 to 7 with addition of 10% FBS (BSA-PL + F ) at Day 4. More blastocysts in BSA-PL--> F and hatching or hatched blastocysts in BSA-PL-->F and BSA-PL+F were obtained. Total cell number in blastocysts derived from BSA-PL-->F and BSA-PL+F were increased. Our results demonstrated that supplementing pyruvate/lactate containing NCSU-23 medium with 0.4% BSA for 4 days and replacing it with 10% FBS for another 3 days improved porcine IVF embryo development.
Kim
Hye-soo
HS
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, South Korea.
Lee
Gab-sang
GS
Hyun
Sang-hwan
SH
Lee
So-hyun
SH
Nam
Dong-hyun
DH
Jeong
Yeon-woo
YW
Kim
Sue
S
Kang
Sung-keun
SK
Lee
Byeong-chun
BC
Hwang
Woo-suk
WS
D000818:Animals; D001769:Blood; D003470:Culture Media; D046508:Culture Techniques; D005314:Embryonic and Fetal Development; D004734:Energy Metabolism; D005307:Fertilization in Vitro / Q000662:veterinary; D005312:Fetal Blood; D019344:Lactic Acid / Q000008:administration & dosage; D019289:Pyruvic Acid / Q000008:administration & dosage; D012710:Serum Albumin, Bovine / Q000008:administration & dosage; D013552:Swine / Q000196:embryology*
D003470:Culture Media; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; D019289:Pyruvic Acid