title: Significance of 8-hydroxy-2'-deoxyguanosine levels in patients with chronic renal failure.
abstract: The aim of this study was to determine the significance of 8-hydroxy-2'-deoxyguanosine (8-OHdG), which is known as a marker of oxidative stress in vivo, in patients with chronic renal failure (CRF). Fifty-one non-dialysed CRF patients (29 men and 22 women; mean +/- SD age, 57.8 +/- 12.8 years) who were under dietary therapy for at least 6 months were enrolled in the study. Both serum and urinary 8-OHdG levels were measured by using high-sensitive enzyme-linked immunosorbent assay (ELISA) kits. We examined the relationship between 8-OHdG levels and clinical indices in patients with CRF. As a result, the serum 8-OHdG level was strongly correlated with serum levels of urea nitrogen (UN; r = 0.58; P < 0.0001), creatinine (Cr; r = 0.53; P < 0.0001), and beta2-microglobulin (beta2-MG; r = 0.54; P < 0.0001). Furthermore, the serum 8-OHdG level was inversely correlated with creatinine clearance (Ccr; r = -0.54; P < 0.0001). In contrast, urinary 8-OHdG level was not correlated with any of the clinical parameters. This is the first report of 8-OHdG level determination in patients with CRF. It is suggested that serum 8-OHdG level is not sufficient as a marker of oxidative damage in patients with CRF, and it should be corrected according to the residual renal function to estimate the accurate degree of oxidative stress.
authors.affiliation: Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama, Japan. akagis50@hotmail.com
authors.forename: Shigeru
authors.initials: S
authors.lastname: Akagi
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Nagake
authors.forename: Junko
authors.initials: J
authors.lastname: Kasahara
authors.forename: Ai
authors.initials: A
authors.lastname: Sarai
authors.forename: Takashi
authors.initials: T
authors.lastname: Kihara
authors.forename: Hisanori
authors.initials: H
authors.lastname: Morimoto
authors.forename: Ai
authors.initials: A
authors.lastname: Yano
authors.forename: Kazushi
authors.initials: K
authors.lastname: Nakao
authors.forename: Kiyohito
authors.initials: K
authors.lastname: Nanba
authors.forename: Haruo
authors.initials: H
authors.lastname: Ichikawa
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Makino
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism*; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine