[Experimental non-culturable Vibrio cholerae eltor and its biological properties].
In experiments with the cultivation of V. cholerae eltor under the conditions of high salt concentration, as well as low temperature and deficiency in nutrient substances, uncultivable forms (UF) of toxigenic and nontoxigenic vibrios were obtained. The absence of growth of seeded vibrios after the filtration of samples (with a filter of 0.22 micron), the preservation of specific antigenic determinants and the initial set of genes, changes in the morphology of cells (small size, coccoid form with the flagella retained) confirm the transition of V. cholerae eltor under study into the uncultivable state which, under unfavorable conditions, more rapidly develops in toxigenic vibrios than in nontoxigenic ones. The analysis of the INT-reductase activity of UF disintegrates revealed that they had endogenic respiration whose activity increased (4.5- to 6.5-fold) in the presence of the exogenic intermediates of the Krebs cycle. The uncultivable forms of the vibrios retain genes responsible for pathogenicity, as well as their antigenic determinants.
Tafel'shteĭn
E E
EE
Research Institute for Plague Control of Siberia and the Far East, Irkutsk, Russia.
Golubinskiĭ
E P
EP
Maramovich
A S
AS
Urbanovich
L Ia
LIa
Burenina
L F
LF
Mironova
L V
LV
Sappo
S G
SG
Markov
E Iu
EIu
Kapustin
Iu M
IuM
Beloborodov
Iu V
IuV
D000222:Adaptation, Physiological; D003470:Culture Media; D010088:Oxidoreductases / Q000378:metabolism; D013696:Temperature; D013778:Tetrazolium Salts; D041241:Vibrio cholerae O1 / Q000166:cytology / Q000502:physiology*; D014776:Virus Cultivation
D003470:Culture Media; D013778:Tetrazolium Salts; C013574:iodonitrotetrazolium; D010088:Oxidoreductases