Marker-assisted pyramiding of brown planthopper (Nilaparvata lugens Stål) resistance genes Bph1 and Bph2 on rice chromosome 12.
Brown planthopper (BPH) (Nilaparvata lugens Stål) is a significant insect pest of rice (Oryza sativa L.). We constructed a gene-pyramided japonica line, in which two BPH resistance genes Bph1 and Bph2 on the long arm of chromosome 12 independently derived from two indica resistance lines were combined through the recombinant selection. The gene-pyramiding was achieved based on the previously constructed high-resolution linkage maps of the two genes. Two co-dominant and four dominant PCR-based markers flanking the loci were used to select for a homozygous recombinant line in a segregating population that was derived from a cross between the parental homozygous single-gene introgression lines. BPH bioassay showed that the resistance level of the pyramided line was equivalent to that of the Bph1-single introgression line, which showed a higher level of resistance than the Bph2-single introgression line. The pyramid line should provide a useful experimental means for studying the fine structure of the chromosomal region covering these two major BPH resistance genes.
Sharma
Prem N
PN
Laboratory of Plant Genetics, Department of Biological and Environmental Science, Faculty of Agriculture, and Division of Life Science, Graduate School of Science and Technology, Kobe University, Kobe, Japan.
Torii
Akihide
A
Takumi
Shigeo
S
Mori
Naoki
N
Nakamura
Chiharu
C
D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D003433:Crosses, Genetic; D008040:Genetic Linkage; D005819:Genetic Markers; D005828:Genetics, Population; D006430:Hemiptera / Q000235:genetics*; D006720:Homozygote; D007564:Japan; D012275:Oryza / Q000469:parasitology*; D010935:Plant Diseases / Q000235:genetics*; D018515:Plant Leaves; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic; D012641:Selection, Genetic
D005819:Genetic Markers