title: Mitogenic activity of Cratylia mollis lectin on human lymphocytes.
abstract: The mitogenic effect of Cratylia mollis seed lectin preparations containing two (Cramoll 1,4) or one molecular form (Cramoll 1) showed activity similar to the well known T-cell mitogen, concanavalin A (Con A). The effect on human lymphocytes was analyzed through a colorimetric assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). Inhibition of lymphocyte proliferation with methyl-alpha-d-mannoside (both preparations) indicated that the mitogenic effect involved carbohydrate lectin binding sites.
authors.affiliation: Universidade Federal de Pernambuco, Centro de Ciências Biológicas (CCB), Departamento de Bioquímica, Rua Prof. Nelson Chaves S/N, 50670-901 Cidade Universitária Recife-PE, Brazil.
authors.forename: Elba V M
authors.initials: EV
authors.lastname: Maciel
authors.forename: Vanduir S
authors.initials: VS
authors.lastname: Araújo-Filho
authors.forename: Mineo
authors.initials: M
authors.lastname: Nakazawa
authors.forename: Yara M
authors.initials: YM
authors.lastname: Gomes
authors.forename: Luana C B B
authors.initials: LC
authors.lastname: Coelho
authors.forename: Maria T S
authors.initials: MT
authors.lastname: Correia
mesh_terms: D001665:Binding Sites; D002241:Carbohydrates / Q000737:chemistry; D002455:Cell Division; D004396:Coloring Agents / Q000494:pharmacology; D003208:Concanavalin A / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D010944:Plants / Q000378:metabolism*; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013997:Time Factors
chemical_list: D002241:Carbohydrates; D004396:Coloring Agents; D037102:Lectins; D013778:Tetrazolium Salts; D013844:Thiazoles; D003208:Concanavalin A; C022616:thiazolyl blue