title: Oxidative stress and enzymatic antioxidant status in patients with nonalcoholic steatohepatitis.
abstract: Oxidative stress is an important pathophysiological mechanism in nonalcoholic steatohepatitis (NASH). To assess whether there are relationships between oxidative stress and antioxidant enzymes in the development of NASH, we investigated oxidative stress by measuring serum malondialdehyde (MDA) and nitric oxide (NO) and antioxidant status by measuring serum glutathione (GSH), glutathione peroxidase (GSH-Px), glutathione reductase (GR), and superoxide dismutase (SOD). The study included 18 patients (13 men, 5 women; mean age 42 yr) with biopsy proven NASH and 16 healthy volunteers (10 men, 6 women; mean age 38 yr). Serum levels of MDA, NO, GSH, GSH-Px, GR and SOD were determined by spectrophotometric methods. Serum levels (mean +/- SD) of MDA (6.7 +/- 1.6 vs 2.8 +/- 1.7 nmol/ml, p 0.0001), NO (135 +/- 28 vs 113 +/- 35 mmol/L, p 0.04), GSH (919 +/- 137 vs 770 +/- 128 mmol/L, p 0.003) were increased in patients with NASH vs controls. Serum levels of GSH-Px (1063 +/- 152 vs 1000 +/- 94 U/L) and GR (47 +/- 22 vs 40 +/- 21 U/L) were not singnificantly different in the patients vs controls. However, the serum level of SOD (1.24 +/- 0.32 vs 1.51 +/- 0.37 U/ml, p: 0.04) was significantly decreased. Impaired antioxidant defense mechanisms may be an important factor in the pathogenesis of NASH. Treatment approaches that affect the antioxidant enzymes may be beneficial in patients with NASH.
authors.affiliation: Department of Gastroenterology, Gaziantep University Medical School, Gaziantep, Turkey. koruk@gantep.edu.tr
authors.forename: Mehmet
authors.initials: M
authors.lastname: Koruk
authors.forename: Seyithan
authors.initials: S
authors.lastname: Taysi
authors.forename: M Cemil
authors.initials: MC
authors.lastname: Savas
authors.forename: Omer
authors.initials: O
authors.lastname: Yilmaz
authors.forename: Fatih
authors.initials: F
authors.lastname: Akcay
authors.forename: Metin
authors.initials: M
authors.lastname: Karakok
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D006505:Hepatitis / Q000097:blood* / Q000201:enzymology / Q000473:pathology; D006801:Humans; D008099:Liver / Q000473:pathology / Q000503:physiopathology; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D000975:Antioxidants; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase