title: The interaction of native DNA with iron(III)- N ,N'-ethylene-bis(salicylideneiminato)-chloride.
abstract: The interaction between native calf thymus deoxyribonucleic acid (DNA) and Fe(III)- N ,N'-ethylene-bis (salicylideneiminato)-chloride, Fe(Salen)Cl, was investigated in aqueous solutions by UV-visible (UV-vis) absorption, circular dichroism (CD), thermal denaturation and viscosity measurements. The results obtained from CD, UV-vis and viscosity measurements exclude DNA intercalation and can be interpreted in terms of an electrostatic binding between the Fe(Salen)(+) cation and the phosphate groups of DNA. The trend of the UV-vis absorption band of the Fe(Salen)Cl complex at different ratios [DNA(phosphate)]/[Fe(Salen)Cl] and the large increase of the melting temperature of DNA in the presence of Fe(Salen)Cl, support the hypothesis of an external electrostatic interaction between the negatively charged DNA double helix and the axially stacked positively charged Fe(Salen)(+) moieties, analogously to what reported for a number of porphyrazines and metal-porphyrazine complexes interacting with DNA.
authors.affiliation: Dipartimento di Chimica Inorganica e Analitica "S Cannizzaro", Università di Palermo, Viale delle Scienze, Parco d'Orleans II, I-90128, Palermo, Italy. asilves@unipa.it
authors.forename: Arturo
authors.initials: A
authors.lastname: Silvestri
authors.forename: Giampaolo
authors.initials: G
authors.lastname: Barone
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Ruisi
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Lo Giudice
authors.forename: Salvatore
authors.initials: S
authors.lastname: Tumminello
mesh_terms: D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D007503:Iron Isotopes; D009691:Nucleic Acid Denaturation; D012545:Schiff Bases / Q000737:chemistry*; D013057:Spectrum Analysis; D014783:Viscosity
chemical_list: C484842:Fe(III)- N ,N'-ethylene-bis (salicylideneiminato)-chloride; D005290:Ferric Compounds; D007503:Iron Isotopes; D012545:Schiff Bases; D004247:DNA