Facile quantification of enantiomeric excess and concentration with indicator-displacement assays: an example in the analyses of alpha-hydroxyacids.
An enantioselective indicator-displacement assay was developed to determine enantiomeric excess (ee) and concentration of alpha-hydroxyacid samples with two independent absorption measurements. The inherent relationship between the absorbance of the indicator-displacement ensemble and the overall concentration and ee of the analyte was established through solution equilibria.
Zhu
Lei
L
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712, USA.
Anslyn
Eric V
EV
D002384:Catalysis; D006880:Hydroxy Acids / Q000737:chemistry*; D007700:Kinetics; D008956:Models, Chemical; D015394:Molecular Structure; D013237:Stereoisomerism
D006880:Hydroxy Acids