Solid phase assays in glycoconjugate research: applications to the analysis of proteoglycans, glycosaminoglycans and metalloproteinases.
Glycoconjugates are a class of macromolecules consisting of different constituents, one of which is sugar moieties. Glycoconjugates comprise the majority of tissue constituents, both intracellular and extracellular. Extracellular glycoconjugates (glycoproteins and proteoglycans) participate in a wide variety of interactions, through which they maintain tissue integrity. Therefore, their analysis or the study of their possible interactions would give evidence for the state of tissues. Since the amounts of some of the extracellular glycoconjugates are usually low or the amounts of tissue to be examined come from biopsies, specific analytical systems are developed for their study, the most familiar being solid phase assays, which have the advantages of analysis of multiple samples on the same time, cheap instrumentation and high specificity.
Ziouti
N
N
Department of Chemistry, Laboratory of Biochemistry, University of Patras, 26500 Patras, Greece.
Triantaphyllidou
I-E
IE
Assouti
M
M
Papageorgakopoulou
N
N
Kyriakopoulou
D
D
Anagnostides
S Th
ST
Vynios
D H
DH
D004797:Enzyme-Linked Immunosorbent Assay; D006001:Glycoconjugates / Q000737:chemistry* / Q000502:physiology; D006025:Glycosaminoglycans / Q000737:chemistry* / Q000378:metabolism; D006115:Greece; D006801:Humans; D020782:Matrix Metalloproteinases / Q000737:chemistry* / Q000378:metabolism; D008722:Methods; D015394:Molecular Structure; D011509:Proteoglycans / Q000737:chemistry* / Q000502:physiology
D006001:Glycoconjugates; D006025:Glycosaminoglycans; D011509:Proteoglycans; D020782:Matrix Metalloproteinases