title: Seroprevalence of Sarcosystis spp. in cattle and buffaloes from the wet and dry zones of Sri Lanka: a preliminary study.
abstract: An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of antibodies to Sarcosystis infection in cattle and buffaloes. Crude antigens derived from cystozoites of Sarcosystis cruzi and antibodies from the sera of naturally infected cattle and buffaloes were used. The assay was validated by determination of the specificity, sensitivity and negative and positive predictive values. Using ELISA, a sero-epidemiological survey for sarcosystosis was carried out in cattle (n = 215) and buffaloes (n = 123) from the wet and dry zones of Sri Lanka. The prevalence of infection in cattle was 69.3%. Prevalence of the infection collectively in both cattle and buffaloes in the dry zone was significantly higher (P < 0.001) than that in the wet zone. The number of sero-positive cattle and buffaloes in the dry zone was significantly higher than that in the wet zone (P < 0.05). Buffaloes in the dry and wet zones showed a greater prevalence of infection (P < 0.05) than cattle.
authors.affiliation: Department of Zoology, Faculty of Science, University of Colombo, Colombo, Sri Lanka.
authors.forename: D G W
authors.initials: DG
authors.lastname: Kalubowila
authors.forename: P V
authors.initials: PV
authors.lastname: Udagama-Randeniya
authors.forename: N A N D
authors.initials: NA
authors.lastname: Perera
authors.forename: R P V J
authors.initials: RP
authors.lastname: Rajapakse
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002020:Buffaloes; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000453:epidemiology* / Q000209:etiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D011237:Predictive Value of Tests; D011891:Rain; D012522:Sarcocystis / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D012523:Sarcocystosis / Q000453:epidemiology / Q000662:veterinary*; D016036:Seroepidemiologic Studies; D013188:Sri Lanka / Q000453:epidemiology
chemical_list: D000907:Antibodies, Bacterial