title: Genetic dissection of complex traits with chromosome substitution strains of mice.
abstract: Chromosome substitution strains (CSSs) have been proposed as a simple and powerful way to identify quantitative trait loci (QTLs) affecting developmental, physiological, and behavioral processes. Here, we report the construction of a complete CSS panel for a vertebrate species. The CSS panel consists of 22 mouse strains, each of which carries a single chromosome substituted from a donor strain (A/J) onto a common host background (C57BL/6J). A survey of 53 traits revealed evidence for 150 QTLs affecting serum levels of sterols and amino acids, diet-induced obesity, and anxiety. These results demonstrate that CSSs greatly facilitate the detection and identification of genes that control the wide diversity of naturally occurring phenotypic variation in the A/J and C57BL/6J inbred strains.
authors.affiliation: Broad Institute, Cambridge, MA 02142, USA.
authors.forename: Jonathan B
authors.initials: JB
authors.lastname: Singer
authors.forename: Annie E
authors.initials: AE
authors.lastname: Hill
authors.forename: Lindsay C
authors.initials: LC
authors.lastname: Burrage
authors.forename: Keith R
authors.initials: KR
authors.lastname: Olszens
authors.forename: Junghan
authors.initials: J
authors.lastname: Song
authors.forename: Monica
authors.initials: M
authors.lastname: Justice
authors.forename: William E
authors.initials: WE
authors.lastname: O'Brien
authors.forename: David V
authors.initials: DV
authors.lastname: Conti
authors.forename: John S
authors.initials: JS
authors.lastname: Witte
authors.forename: Eric S
authors.initials: ES
authors.lastname: Lander
authors.forename: Joseph H
authors.initials: JH
authors.lastname: Nadeau
mesh_terms: D000596:Amino Acids / Q000097:blood; D000818:Animals; D001007:Anxiety / Q000235:genetics; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian / Q000235:genetics*; D003433:Crosses, Genetic; D004032:Diet; D005260:Female; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D018895:Microsatellite Repeats; D009765:Obesity / Q000235:genetics / Q000503:physiopathology; D010641:Phenotype; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable; D013261:Sterols / Q000097:blood; D015430:Weight Gain
chemical_list: D000596:Amino Acids; D013261:Sterols