title: [The inhibitive effect of tea polyphenols on oxidized low density lipoprotein intake in cultured rat mesangial cells].
abstract: OBJECTIVE
  To study the inhibitive effect of tea polyphenols (TP) on oxidized low density lipoprotein (OX-LDL) intake in cultured rat mesangial cells.
  
  
  METHODS
  Rat mesangial cells incubated in 80 mg.L-1 OX-LDL were treated with TP at different concentrations. Total cholesterol (TC) content of the mesangial cells and proportion of mesangial cells containing lipid droplets were measured, and ultrastructure observation was performed.
  
  
  RESULTS
  Foam cells were observed in mesangial cells stimulated by OX-LDL, and both the TC content and the proportion of lipid-droplet-containing cell were higher in these cells than in mesangial cells cultured in lipid free conditions. There were concentration-dependent decreases both in TC content and lipid-droplet-containing cell proportions when treated with TP from 40 ng.L-1 to 40 mg.L-1. The number of lipid droplets was also decreased in TP treated mesangial cells.
  
  
  CONCLUSIONS
  TP has an inhibitive effect on OX-LDL intake in cultured rat mesangial cells, which is in a concentration-dependent manner from 40 ng.L-1 to 40 mg.L-1.
authors.affiliation: Department of Nephrology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Gan
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D005487:Foam Cells / Q000378:metabolism; D005920:Glomerular Mesangium / Q000166:cytology / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008297:Male; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D059808:Polyphenols; D051381:Rats; D017208:Rats, Wistar; D013662:Tea / Q000737:chemistry
chemical_list: D005419:Flavonoids; D008077:Lipoproteins, LDL; D010636:Phenols; D059808:Polyphenols; D013662:Tea; C099252:oxidized low density lipoprotein; D002784:Cholesterol