title: [Screening and identification of human telomerase reverse transcriptase epitopes from random phage display peptide library].
abstract: OBJECTIVE
  To identify and characterize the epitopes of human telomerase reverse transcriptase (hTERT).
  
  
  METHODS
  A random phage displayed dodecapeptide library was screened with anti-hTERT antibody. The selected phage clones were identified by sandwich enzyme-linked immunosorbent assay, anti-hTERT antibody blocking assay and competitive inhibition assay.
  
  
  RESULTS
  After 3 rounds of screening, 13 of 24 randomly selected phage clones were identified as positive clones that could specifically bind to anti-hTERT antibody but not to normal mouse IgG. Amino acid sequences deduced from DNA sequences showed 11 different sequences with high percentage of histone and hydrophilic amino acids.
  
  
  CONCLUSIONS
  Eleven epitopes have been obtained that can effectively mimic hTERT, which will be useful for the research of small-molecule inhibitor targeting at hTERT.
authors.affiliation: Department of Pharmacology, First Military Medical University, Guangzhou 510515, China. miaoph@fimmu.com
authors.forename: Pei-hong
authors.initials: PH
authors.lastname: Miao
authors.forename: Bei-yi
authors.initials: BY
authors.lastname: Liu
authors.forename: Shan-gen
authors.initials: SG
authors.lastname: Zheng
authors.forename: Jian-xin
authors.initials: JX
authors.lastname: Pang
authors.forename: Jiang-ping
authors.initials: JP
authors.lastname: Xu
mesh_terms: D001483:Base Sequence; D004268:DNA-Binding Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D006801:Humans; D019151:Peptide Library; D019098:Telomerase / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
chemical_list: D004268:DNA-Binding Proteins; D000939:Epitopes; D019151:Peptide Library; D019098:Telomerase