Real-time polymerase chain reaction for detecting SARS coronavirus, Beijing, 2003.
During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3' end of the viral genome, was established to detect and identify the SARS-associated coronavirus. We describe the use of this assay to screen >700 clinical samples.
Zhai
Junhui
J
Institute of Microbiology and Epidemiology, Academy of Military Sciences, Beijing, China.
Briese
Thomas
T
Dai
Erhei
E
Wang
Xiaoyi
X
pang
Xin
X
Du
Zongmin
Z
Liu
Haihong
H
Wang
Jin
J
Wang
Hongxia
H
Guo
Zhaobiao
Z
Chen
Zeliang
Z
Jiang
Lingxiao
L
Zhou
Dongsheng
D
Han
Yanping
Y
Jabado
Omar
O
Palacios
Gustavo
G
Lipkin
W Ian
WI
Tang
Ruifu
R
D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004196:Disease Outbreaks; D005243:Feces / Q000821:virology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D012367:RNA, Viral / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D045169:Severe Acute Respiratory Syndrome / Q000453:epidemiology / Q000276:immunology / Q000821:virology; D013997:Time Factors
D000914:Antibodies, Viral; D017931:DNA Primers; D004279:DNA, Viral; D012367:RNA, Viral