The use of precision glass syringes and a noncontact microsolenoid dispenser for the production of high-throughput low-density arrays.
The advantages of using nondisposable precision glass syringes in automated high-throughput microdispensers and a single-channel noncontact microsolenoid dispenser in creating highly uniform and reproducible protein, nucleic acid, and organic compound array filters, slides, and plates are described. Using a HydraPlus-One system, protein solutions of up to 100 mg/mL (in 100 nL) can be spotted onto slides and plates at a speed of 0.6 s per spot. Using the Hydra microdispenser and Tango liquid handling system, as little as 100 nL of polymerase chain reaction (PCR)-amplified human cancer-related genes, housekeeping genes, and protein solutions can be spotted onto nylon membranes, coated slides, or plates. Using these microdispensers equipped with syringes, up to 6144 uniform and reproducible spots per membrane and up to 1000 spots per slide, with a precision variation of less than 10%, were printed at a speed of as fast as 2.5 min per membrane.
Azarani
Arezou
A
Protogen Consulting, San Jose, CA, USA.
D000199:Actins / Q000032:analysis; D020869:Gene Expression Profiling / Q000379:methods; D005898:Glass; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000032:analysis; D006801:Humans; D008904:Miniaturization; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods*; D009857:Oncogenes; D015203:Reproducibility of Results; D013594:Syringes
D000199:Actins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases