Synergistic efficacy of magnesium sulfate and FK506 on cerebral ischemia-induced infarct volume in gerbil.
BACKGROUND
In this study, we administered intraperitoneal magnesium sulfate (90 mg/kg), either alone or in combination with FK506 (0.5 mg/kg) in order to investigate the neuroprotective synergetic effects of both, 30 min before permanent occlusion of the middle cerebral artery (MCA) in gerbil.


METHODS
Postmortem infarct volume was determined by quantitative image analysis of triphenyltetrazolium-stained brain sections, collected at 1 h, 3 h and 24 h after initiation of cerebral ischemia, to measure consistent infarct volumes of the post -ischemic gerbil brain. Twenty-four male gerbils were intraperitoneally injected with MgSO4 (n=7), FK506 (n=8), or MgSO4 +FK506 (n=9), 30 min before permanent MCA occlusion. The brains of the treated groups were stained at 24 h after initiation of cerebral ischemia.


RESULTS
Significant and consistent infarct volumes (78.3 +/- 3.6 mm3) were observed in the 24 H control group. The infarct volume in the MgSO4 + FK506 group was significantly reduced at each slice, compared to the untreated 24 H control group (P<0.05). Magnesium sulfate reduced total infarct volume by 25.4% (P<0.05), FK506 by 41% (P<0.05), and MgSO4 +FK506 by 50.5% (P<0.05). Also, the infarct volume (38.7 +/- 5.2 mm3) in the MgSO4 + FK506 group was the smallest in all the treated groups, near the volumes seen in the untreated 3H group.


CONCLUSIONS
Acute administration of a combination of MgSO4 and FK506 has a synergetic efficacy against brain damage by permanent focal cerebral ischemia.
Chung
Shu-Ying
SY
Department of Food and Nutrition, Providence University, Taichung 433, Taiwan, ROC.
Lin
Jing-Ying
JY
Lin
Ming-Cheng
MC
Liu
Hui-Ming
HM
Wang
Ming-Fu
MF
Cheng
Fu-Chou
FC
D000818:Animals; D002531:Cerebellum / Q000187:drug effects / Q000473:pathology*; D002544:Cerebral Infarction / Q000188:drug therapy / Q000473:pathology*; D004357:Drug Synergism; D005849:Gerbillinae; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008278:Magnesium Sulfate / Q000494:pharmacology*; D008297:Male; D016559:Tacrolimus / Q000494:pharmacology*
D007166:Immunosuppressive Agents; D008278:Magnesium Sulfate; D016559:Tacrolimus