title: Peptides from anchovy sauce induce apoptosis in a human lymphoma cell (U937) through the increase of caspase-3 and -8 activities.
abstract: Various biological activities of peptides have been found. In this study, the induction of apoptosis in a human lymphoma cell line (U937) by peptide fraction separated from anchovy sauce was studied using biochemical and flow cytometric methods. After U937 was treated with a hydrophobic peptide fraction of the anchovy sauce, a sub-G1 peak, which represents apoptosis, was found in the cell cycle analysis. The apoptosis in the peptide-treated U937 cell was also confirmed by apoptotic DNA fragmentation. Furthermore, an increase of caspase-3 and -8 activities was observed. Our results suggest that the peptide fraction separated from the anchovy sauce may have cancer chemopreventive effects through the induction of apoptosis in cancer cells.
authors.affiliation: Department of Food Science and Technology and School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Young Gon
authors.initials: YG
authors.lastname: Lee
authors.forename: Ji Yeon
authors.initials: JY
authors.lastname: Kim
authors.forename: Ki Won
authors.initials: KW
authors.lastname: Lee
authors.forename: Kyoung Heon
authors.initials: KH
authors.lastname: Kim
authors.forename: Hyong Joo
authors.initials: HJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D053181:Caspase 8; D020169:Caspases / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D005399:Fishes; D005511:Food Handling; D006801:Humans; D007700:Kinetics; D010455:Peptides / Q000302:isolation & purification / Q000633:toxicity*; D020298:U937 Cells
chemical_list: D010455:Peptides; C505407:CASP3 protein, human; C505530:CASP8 protein, human; D053148:Caspase 3; D053181:Caspase 8; D020169:Caspases