title: Oxidative stress indices in seminal plasma, as measured by the thermochemiluminescence assay, correlate with sperm parameters.
abstract: OBJECTIVE
  To examine the effect of oxidation of proteins and lipids, as measured by a novel thermochemiluminescence (TCL) analyzer, and to evaluate the correlation between TCL indices in seminal plasma and sperm parameters.
  
  
  METHODS
  Experimental and prospective clinical studies.
  
  
  METHODS
  An infertility unit.
  
  
  METHODS
  One hundred forty-eight men undergoing semen analysis.
  
  
  METHODS
  Bovine serum albumin (BSA) and linolenic acid were oxidized and tested by TCL, protein carbonyls, and conjugated dienes assays. All participants underwent semen analysis. Seminal plasma was tested by TCL and conjugated dienes.
  
  
  METHODS
  Thermochemiluminescence indices before and after oxidation of BSA and linolenic acid, compared with protein carbonyl and conjugated dienes indices. Correlation between semen parameters and TCL and conjugated dienes indices in seminal plasma.
  
  
  RESULTS
  Oxidation of BSA and linolenic acid was marked by characteristic changes in their TCL curve pattern and an increase in the levels of protein carbonyls and conjugated dienes. Among 125 sperm-containing semen samples, the TCL curve exhibited two patterns: a positive relative ratio curve (group A, 87 patients) and a negative relative ratio curve (group B, 38 patients). Sperm concentration was lower and total motile sperm and rapid motile sperm were fewer in group B. A significant correlation was found between TCL indices, conjugated dienes, and sperm quality in group B.
  
  
  CONCLUSIONS
  Oxidation affects TCL curve pattern of proteins and lipids in a characteristic manner. Thermochemiluminescence indices in seminal plasma closely correlate with sperm characteristics among patients with sperm disturbances, and it might serve as a tool in the evaluation, treatment, and monitoring of subfertile men.
authors.affiliation: IVF Unit, Department of Obstetrics and Gynecology, Carmel Medical Center, 7 Michal Street, Haifa, Israel.
authors.forename: Arie
authors.initials: A
authors.lastname: Lissak
authors.forename: Zofnat
authors.initials: Z
authors.lastname: Wiener-Megnazi
authors.forename: Abraham Zeev
authors.initials: AZ
authors.lastname: Reznick
authors.forename: Sergei
authors.initials: S
authors.lastname: Shnizer
authors.forename: David
authors.initials: D
authors.lastname: Ishai
authors.forename: Bronislava
authors.initials: B
authors.lastname: Grach
authors.forename: Shirly
authors.initials: S
authors.lastname: Lahav-Baratz
authors.forename: Hanna
authors.initials: H
authors.lastname: Shiloh
authors.forename: Mara
authors.initials: M
authors.lastname: Koifman
authors.forename: Martha
authors.initials: M
authors.lastname: Dirnfeld
mesh_terms: D000818:Animals; D002417:Cattle; D004867:Equipment Design; D004864:Equipment and Supplies; D006801:Humans; D007958:Leukocyte Count; D008163:Luminescent Measurements; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011446:Prospective Studies; D012661:Semen / Q000166:cytology / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013076:Sperm Count; D013081:Sperm Motility; D017962:alpha-Linolenic Acid / Q000378:metabolism
chemical_list: D017962:alpha-Linolenic Acid; D012710:Serum Albumin, Bovine