[Study on plant tissue culture of Cornus officinalis].
OBJECTIVE
To set up the optimums of tissue culture for Cornus officinalis.


METHODS
A section of stems of the fine varieties of Cornus officinalis were strictly sterilized, cultivated and geminated in medium with addition of different kinds of hormone.


CONCLUSIONS
The WPM medium with 6-BA 2.0 mg.L-1 + ZT 0.1 mg.L-1 + NAA 0.1 mg.L-1 was the optimum medium for the multiplication of adventitious buds. The WPM medium with 6-BA 1.0 mg.L-1 + ZT 0.1 mg.L-1 + GA3 0.5 mg.L-1 + NAA 0.1 mg.L-1 was the optimum medium for cultivation in strength. The plantlet could root well in 1/2 MS medium with IBA 1.0 mg.L-1 + 6-BA 0.1 mg.L-1.
Xue
Jian-ping
JP
Department of Biology, Huaibei Coal Industry Teacher's College, Huaibei 235000, Anhui, China. xuejp2000@yahoo.com.cn
Zhang
Ai-min
AM
Wang
Yue-hui
YH
Sheng
Wei
W
D029768:Cornus / Q000254:growth & development*; D003470:Culture Media; D046508:Culture Techniques; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000254:growth & development; D018547:Plant Stems / Q000254:growth & development; D010946:Plants, Medicinal / Q000254:growth & development*
D003470:Culture Media; D010937:Plant Growth Regulators