Determination of myo-inositol in biological samples by liquid chromatography-mass spectrometry.
Due to the absence of HPLC methods to determine myo-inositol using mass detection and considering its sensitivity and selectivity, a high performance liquid chromatography-mass spectrometry method for the analysis of myo-inositol is described and applied to its direct determination in urine and saliva samples. Successful resolution of myo-inositol and its related substances was achieved with a stationary phase Aminex HPX-87C Column with milli-Q water as mobile phase and 5 mM ammonium acetate added post-column. The detector counted positive ions by monitoring m/z = 198, which corresponds to the myo-inositol adduct with ammonium cation. Urine and saliva samples were previously purified by passing through an anion-exchange resin. Concentrations as low as 138 and 461 microg/l in saliva and urine could be respectively quantified. Intra-day R.S.D. ranged from 0.83 to 1.02%, whereas inter-day R.S.D. was between 1.54 and 3.58%.
Perelló
J
J
Laboratory of Renal Lithiasis Research, Faculty of Sciences, Institute of Health Sciences Research (IUNICS), University of Balearic Islands, Ctra. Valldemossa, km 7.5, 07122 Palma de Mallorca, Spain.
Isern
B
B
Costa-Bauzá
A
A
Grases
F
F
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007294:Inositol / Q000652:urine*; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D007294:Inositol