Glycoprotein of nonpathogenic rabies viruses is a major inducer of apoptosis in human jurkat T cells.
This study sought to identify the RV protein that causes apoptosis. For this purpose, we first compared the ability of G and N proteins of a pathogenic and a nonpathogenic strain to trigger apoptosis of Jurkat rtTA by using an inducible Tet-on expression system. Then we analyzed apoptosis induced by a reverse genetic-engineered recombinant rabies virus in which the G gene from a nonpathogenic strain was replaced by its pathogenic strain counterpart. No other virus proteins than G of nonpathogenic RV strains induce apoptosis, and the G polypeptide of RV is a critical determinant for apoptosis in human cells.
Lay
Stéphanie
S
Unité de NeuroImmunologie Virale, Département de Neuroscience, Institut Pasteur, Paris, France.
Préhaud
Christophe
C
Dietzschold
Bernhard
B
Lafon
Monique
M
D000956:Antigens, Viral; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D006023:Glycoproteins / Q000502:physiology*; D006801:Humans; D019169:Jurkat Cells; D011820:Rabies virus / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology / Q000821:virology; D014759:Viral Envelope Proteins / Q000502:physiology*
D000956:Antigens, Viral; D006023:Glycoproteins; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C064951:glycoprotein G, Rabies virus