Isolation and characterization of a strain of Bacillus thuringiensis subsp. morrisoni PG-14 encoding delta-endotoxin Cry1Ac.
Bacillus thuringiensis 656-3, isolated from a soil sample collected at mushroom houses, showed high toxicity to mushroom flies, Lycoriella mali and Coboldia fuscipes. B. thuringiensis 656-3 produced bipyramidal inclusions and reacted with the H antiserum of B. thuringiensis subsp. morrisoni (H8a8b). The plasmid and protein profiles of B. thuringiensis 656-3 were similar to those of its reference strain, subsp. morrisoni PG-14. However, PCR analysis using cry gene primers showed that B. thuringiensis 656-3, unlike its reference strain, had cry4A, cry4B, cry10A, cry11A, and cry1Ac genes, suggesting that B. thuringiensis 656-3 was a unique strain with respect to gene type. In addition, B. thuringiensis 656-3 showed a high level of toxicity against mushroom flies, L. mali and C. fuscipes.
Choi
Yong Soo
YS
Division of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Korea.
Cho
Eun Sook
ES
Je
Yeon Ho
YH
Roh
Jong Yul
JY
Chang
Jin Hee
JH
Li
Ming Shun
MS
Seo
Sook Jae
SJ
Sohn
Hung Dae
HD
Jin
Byung Rae
BR
D000363:Agaricales; D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism / Q000648:ultrastructure; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001427:Bacterial Toxins; D001681:Biological Assay; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004175:Diptera / Q000378:metabolism; D004731:Endotoxins / Q000235:genetics* / Q000378:metabolism; D006460:Hemolysin Proteins; D008855:Microscopy, Electron, Scanning; D010572:Pest Control, Biological; D016133:Polymerase Chain Reaction; D012988:Soil Microbiology
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis