title: Identification of an iron-binding protein of the Dps family expressed by Streptococcus thermophilus.
abstract: Streptococcus thermophilus PB18 can grow between 20 degrees and 52 degrees C and is resistant to various stresses such as heat, acidic or cold shock. During cold shock, a protein of 21.5 kDa was previously shown to be induced in S. thermophilus. In addition to its cold-shock induction, 2D-PAGE revealed that the 21.5-kDa protein was also expressed during the stationary phase of growth. The recent access to the genome sequence of S. thermophilus LMG18311 allowed the identification of a 173-amino acid protein displaying a strong homology between the 21.5-kDa protein and members of the Dps family of proteins. Specific staining of non-denaturing polyacrylamide gel electrophoresis (ND-PAGE) followed by two-dimensional PAGE (2D-PAGE) showed that the 21.5-kDa protein was an iron-binding protein.
authors.affiliation: Laboratoire des BioSciences de l'Aliment , U.C. INRA n(o)885, Faculté des Sciences et Techniques, Université Henri Poincaré-Nancy 1, B.P. 239, 54506 Vandoeuvre-lès-Nancy Cedex, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Nicodème
authors.forename: Clarisse
authors.initials: C
authors.lastname: Perrin
authors.forename: Pascal
authors.initials: P
authors.lastname: Hols
authors.forename: Patrice
authors.initials: P
authors.lastname: Bracquart
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Gaillard
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D003080:Cold Temperature; D015180:Electrophoresis, Gel, Two-Dimensional; D033862:Iron-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016415:Sequence Alignment; D013291:Streptococcus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D033862:Iron-Binding Proteins