[Ginkgo flavones in in vitro metabolism and its clinical application].
OBJECTIVE
To develop a method for assaying Ginkgo flavones in rat hepatical microsome.


METHODS
Quercetin, isorhamnetin and keampferol were added to microsome incubate and incubated for a given time then extracted with ether-acetone. After evaporated, the residue was reconstituted with 100 microL of phosphate buffer solution (pH 2.0)-tetrahydrofuran-methanol-isopropanol (60:15:10:20). An aliquot of 20 microL was injected into the HPLC system. According to the result of estimate by means of HPLC, the results of metabolism of Ginkgo flavones in different conditions was compared.


RESULTS
The assay was linear over the rang of 0.2-8 mg.L-1 for Ginkgo flavones. The limit of quantification was 0.1 mg.L-1 (n = 3). The recoveries of three components of Ginkgo flavones were 99.9%-113.8% for quercetin (RSD < 0.8%), 100.8%-117.3% for isorhamnetin (RSD < 1.9%) and 100.7%-116.5% for keampferol (RSD < 1.03%, n = 5).


CONCLUSIONS
The method is simple, fast and accurate. It can be used for investigation of the metabolism of Ginkgo flavones.
Zhu
Min
M
Department of Clinical Pharmacy, Second Hospital of Hangzhou City, Hangzhou 310015, China.
Yao
Tong-wei
TW
Zeng
Su
S
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D044948:Flavonols / Q000302:isolation & purification / Q000378:metabolism*; D020441:Ginkgo biloba / Q000737:chemistry*; D066298:In Vitro Techniques; D044949:Kaempferols / Q000302:isolation & purification / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011794:Quercetin / Q000302:isolation & purification / Q000378:metabolism* / Q000031:analogs & derivatives; D051381:Rats
D044948:Flavonols; D044949:Kaempferols; C047368:3-methylquercetin; C006552:kaempferol; D011794:Quercetin