title: Expression, purification, crystallization and preliminary crystallographic studies of the Enterococcus faecalis cytolysin repressor CylR2.
abstract: The expression of an exotoxin called cytolysin contributes to the virulence of Enterococcus faecalis, one of the organisms responsible for antibiotic resistant infections acquired in hospitals. The DNA-binding protein CylR2 is a transcriptional repressor of cytolysin. At a specific cell density, cytolysin triggers signaling events, which result in the dissociation of CylR2 from its DNA-binding site. CylR2 was overexpressed in Escherichia coli and purified and crystals diffracting to 1.9 A were obtained in two different crystal forms. One crystal form belongs to space group P4(1), with unit-cell parameters a = 63.7, b = 63.7, c = 41.2 A, alpha = beta = gamma = 90 degrees, and the other belongs to space group P1, with unit-cell parameters a = 36.9, b = 45.0, c = 47.7 A, alpha = 67, beta = 90, gamma = 66 degrees.
authors.affiliation: Department for NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
authors.forename: Adelia
authors.initials: A
authors.lastname: Razeto
authors.forename: Karin
authors.initials: K
authors.lastname: Giller
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Haas
authors.forename: Michael S
authors.initials: MS
authors.lastname: Gilmore
authors.forename: Markus
authors.initials: M
authors.lastname: Zweckstetter
authors.forename: Stefan
authors.initials: S
authors.lastname: Becker
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D001430:Bacteriocins; D003001:Cloning, Molecular; D003460:Crystallization / Q000379:methods*; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000737:chemistry*; D013293:Enterococcus faecalis / Q000737:chemistry*; D007454:Iodides; D012097:Repressor Proteins / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D001430:Bacteriocins; D004268:DNA-Binding Proteins; D007454:Iodides; D012097:Repressor Proteins; C120351:cytolysin, Enterococcus faecalis