The hydrophobic domain of the Mycobacterial Erp protein is not essential for the virulence of Mycobacterium tuberculosis.
Erp (exported repetitive protein) is a member of a mycobacterium-specific family of extracellular proteins. A hydrophobic region that is localized at the C-terminal domain and that represents a quarter of the protein is highly conserved across species. Here we show that this hydrophobic region is not essential for restoring the virulence and tissue damage of an erp::aph mutant strain of M. tuberculosis as assessed by bacterial counts and lung histology analysis in a mouse model of tuberculosis.
Kocíncová
Dana
D
Unité de Génétique Mycobactérienne, Institut Pasteur, Paris Cedex 15, F-75724, France.
Sondén
Berit
B
Bordat
Yann
Y
Pivert
Elisabeth
E
de Mendonça-Lima
Leila
L
Gicquel
Brigitte
B
Reyrat
Jean-Marc
JM
D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008168:Lung / Q000382:microbiology / Q000473:pathology*; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000472:pathogenicity*; D014397:Tuberculosis, Pulmonary / Q000382:microbiology / Q000473:pathology*; D014774:Virulence
D001426:Bacterial Proteins; C096737:ERP protein, Mycobacterium tuberculosis; D008565:Membrane Proteins