Modulation of allergic and immune complex-induced lung inflammation by bradykinin receptor antagonists.
OBJECTIVE
The effect of bradykinin (B(1) or B(2)) receptor antagonists was studied in allergic and immune-complex-induced lung inflammation.


METHODS
Lungs of BALB/c mice were examined 24 h after induction of lung inflammation, either allergic (ovalbumin-sensitized submitted to two aerosol of antigen, one week apart) or immune-complex induced (intratracheal instillation of IgG antibodies followed by intravenous antigen). The bradykinin B(2) receptor antagonist, HOE-140 or bradykinin B(1) receptor antagonist, R-954 were given intraperitoneally (100 microg/kg), 30 min before induction.


RESULTS
In allergic inflammation, pre-treatment with R-954 reduced eosinophil infiltration into the lungs, mucus secretion and the airway hyperreactivity to methacholine. Pre-treatment with HOE-140 increased eosinophil infiltration but did not affect the other parameters. In immune-complex inflammation, HOE-140 increased neutrophil infiltration but not their activation nor the hemorrhagic lesions. R-594 pre-treatment did not change the parameters examined.


CONCLUSIONS
These results show important modulatory effects of bradykinin B(1) and B(2) receptor antagonists in both models of lung inflammation.
Gama Landgraf
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Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 1730, 05508-900 São Paulo, Brazil.
Jancar
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Steil
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Sirois
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D000818:Animals; D001920:Bradykinin / Q000031:analogs & derivatives* / Q000627:therapeutic use; D065169:Bradykinin B1 Receptor Antagonists; D065094:Bradykinin B2 Receptor Antagonists; D065168:Bradykinin Receptor Antagonists; D016084:Bronchoconstriction / Q000187:drug effects; D004804:Eosinophils / Q000473:pathology; D006967:Hypersensitivity; D007105:Immune Complex Diseases; D008297:Male; D016210:Methacholine Chloride / Q000008:administration & dosage; D051379:Mice; D008807:Mice, Inbred BALB C; D009093:Mucus / Q000378:metabolism; D010047:Ovalbumin / Q000276:immunology; D011014:Pneumonia / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control*
C476306:Ac-Orn-(Oic2, alpha-MePhe5, D-betaNal7, Ile8)desArg9-bradykinin; D065169:Bradykinin B1 Receptor Antagonists; D065094:Bradykinin B2 Receptor Antagonists; D065168:Bradykinin Receptor Antagonists; D016210:Methacholine Chloride; C065679:icatibant; D010047:Ovalbumin; D001920:Bradykinin