title: MMNTP-a new tailor-made modular derivatization agent for the selective determination of isocyanates and diisocyanates.
abstract: The synthesis of a new tailor-made derivatization agent for the selective determination of (di)isocyanates is presented. Starting from cyanuric chloride, the reagent 4-methoxy-6-(4-methoxy-1-naphthyl)-1,3,5-triazine-2-(1-piperazine)(MMNTP) is synthesized by subsequent substitution of the three chlorine atoms. This new derivatization agent and the five urea derivatives of phenylisocyanate (PI), hexamethylene-diisocyanate (HDI), toluene-2,4-diisocyanate (2,4-TDI), toluene-2,6-diisocyanate (2,6-TDI) and methylenebisphenyl-4,4-diisocyanate (MDI) show good spectroscopic properties with small compound-to-compound variabilities (RSD([epsilon])= 5.3 %, RSD(relative fluorescence)= 9.4 %). Therefore, using UV detection, a single calibration is needed for the quantification of all diisocyanates and isocyanates respectively. For separation and analysis a HPLC method with a RP column and a binary gradient is presented. All derivatives are separated and show low limits of detection. In addition to the good spectroscopic properties and low limits of detection, good reactivity for the derivatizations at room temperature is observed. The aromatic diisocyanates can be measured immediately whereas aliphatic diisocyanates need 2 h incubation. These advantages make MMNTP a powerful and versatile derivatization agent for (di)isocyanates which is demonstrated by a real sample with solid phase sampling, where the reagent is coated on a sorbent.
authors.affiliation: University of Münster, Institut für Arbeitsmedizin, Robert-Koch-Str. 51, D-48149 Münster, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Werlich
authors.forename: Heidi
authors.initials: H
authors.lastname: Stockhorst
authors.forename: Ute
authors.initials: U
authors.lastname: Witting
authors.forename: Norbert
authors.initials: N
authors.lastname: Binding
mesh_terms: D000393:Air Pollutants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004784:Environmental Monitoring / Q000379:methods; D007202:Indicators and Reagents; D017953:Isocyanates / Q000032:analysis*
chemical_list: D000393:Air Pollutants; D007202:Indicators and Reagents; D017953:Isocyanates