Determination of morphine by capillary zone electrophoresis immunoassay combined with laser-induced fluorescence detection.
A competitive immunoassay for detecting morphine in bio-samples was established by capillary zone electrophoresis combined with laser-induced fluorescence detection (CZE-LIF). The antigen of morphine was labeled with isothiocyano-fluorescein (FITC) and then incubated with morphine monoclonal antibody and samples. The linear range was 50-1000 ng/mL, which was suitable for clinical and forensic applications. The detection limit can reach 40 ng/mL, based on S/N = 2. The recoveries of morphine from serum were satisfactory.
Mi
Jian-Qiu
JQ
The Key Lab of Bioorganic Chemistry and Molecular Engineering, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing, PR China.
Zhang
Xin-Xiang
XX
Chang
Wen-Bao
WB
D000918:Antibody Specificity; D019075:Electrophoresis, Capillary / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005453:Fluorescence; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007834:Lasers; D009020:Morphine / Q000097:blood*; D012680:Sensitivity and Specificity
D009020:Morphine; D016650:Fluorescein-5-isothiocyanate