title: Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins.
abstract: Using Nalpha,Nalpha-bis(carboxymethyl)lysine to react with FePt magnetic nanoparticles, we synthesized the FePt-NTA conjugate, which immobilizes Ni2+ ions and selectively binds to histidine-tagged proteins at concentration as low as 0.5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins.
authors.affiliation: Department of Chemistry, Department of Physics, and Bioengineering Program, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China. chbingxu@ust.hk
authors.forename: Chenjie
authors.initials: C
authors.lastname: Xu
authors.forename: Keming
authors.initials: K
authors.lastname: Xu
authors.forename: Hongwei
authors.initials: H
authors.lastname: Gu
authors.forename: Xiaofen
authors.initials: X
authors.lastname: Zhong
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Guo
authors.forename: Rongkun
authors.initials: R
authors.lastname: Zheng
authors.forename: Xixiang
authors.initials: X
authors.lastname: Zhang
authors.forename: Bing
authors.initials: B
authors.lastname: Xu
mesh_terms: D000497:Alloys / Q000737:chemistry; D006639:Histidine / Q000737:chemistry*; D007501:Iron / Q000737:chemistry; D008280:Magnetics; D036103:Nanotechnology / Q000379:methods*; D009571:Nitrilotriacetic Acid / Q000737:chemistry*; D010316:Particle Size; D010984:Platinum / Q000737:chemistry; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000497:Alloys; D011506:Proteins; D010984:Platinum; D006639:Histidine; D007501:Iron; D009571:Nitrilotriacetic Acid