Tec1p-independent activation of a hypha-associated Candida albicans virulence gene during infection.
The Tec1p transcription factor is involved in the expression of hypha-specific genes in Candida albicans. Although the induction of the hypha-associated SAP5 gene by serum in vitro depends on Tec1p, deletion of all Tec1p binding site consensus sequences from the SAP5 promoter did not affect its activation. In two different animal models of candidiasis, the SAP5 promoter was induced even in a Deltatec1 deletion mutant, demonstrating that the requirement for Tec1p in gene expression in C. albicans depends on the environmental conditions within the host.
Staib
Peter
P
Institut für Molekulare Infektionsbiologie, Universität Würzburg, D-97070 Wuerzburg, Germany.
Binder
Ayfer
A
Kretschmar
Marianne
M
Nichterlein
Thomas
T
Schröppel
Klaus
K
Morschhäuser
Joachim
J
D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000235:genetics / Q000378:metabolism*; D002176:Candida albicans / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D002177:Candidiasis / Q000382:microbiology / Q000503:physiopathology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D005656:Fungal Proteins; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D006801:Humans; D025301:Hyphae / Q000254:growth & development*; D051379:Mice; D010538:Peritonitis / Q000382:microbiology / Q000503:physiopathology*; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014774:Virulence
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C489432:TEC1 protein, Candida albicans; D014157:Transcription Factors; D016282:Aspartic Acid Endopeptidases; C086390:SAP1 protein, Candida albicans