Tsetse flies are attracted to the invasive plant Lantana camara.
In tsetse both sexes feed exclusively on the blood of vertebrates for a few minutes every 2-3 days. Tsetse flies seek cover from high temperatures to conserve energy and plants provide shelter for tsetse in all the biotopes they occupy. Recently, tsetse have taken cover in plantations and under the invasive bush Lantana camara that has invaded large areas of the tsetse fly belt of Africa. Flies from such refugia are implicated in sleeping sickness epidemics. In a wind tunnel we show that both foliage and an extract of volatiles from foliage of L. camara attract three tsetse spp. from different habitats: Glossina fuscipes fuscipes (riverine), G. brevipalpis (sylvatic) and G. pallidipes (savannah). Gas chromatography analysis of volatiles extracted from leaves and flowers of L. camara coupled to electroantennograme recordings show that 1-octen-3-ol and beta-caryophyllene are the major chemostimuli for the antennal receptor cells of the three tsetse spp. studied. A binary mixture of these products attracted these flies in the wind tunnel. The gas chromatography linked electroantennograme analysis of the L. camara extracts also show that the antennal receptor cells of the three tsetse spp. respond similarly to groups of volatiles derived from the major biosynthetic and catabolic pathways of plants, i.e. to mono- and sesquiterpenes, to lipoxidation products and to aromatics. Mixtures of these plant volatiles also attracted tsetse in the wind tunnel. These findings show that tsetse flies have conserved a strong sensitivity to volatile secondary products of plants, underlining the fundamental role of vegetation in tsetse survival.
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Institute of Zoology, University of Neuchâtel, Rue Emile Argand 11, 2007 Neuchâtel, Switzerland. patrick.guerin@unine.ch
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D000818:Animals; D002633:Chemotaxis / Q000502:physiology; D002849:Chromatography, Gas; D035264:Flowers / Q000737:chemistry; D032410:Lantana / Q000737:chemistry*; D000442:Octanols / Q000032:analysis; D009812:Odorants; D010465:Perception / Q000502:physiology*; D010675:Pheromones / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry*; D000081223:Polycyclic Sesquiterpenes; D012679:Sense Organs / Q000502:physiology*; D012717:Sesquiterpenes / Q000032:analysis; D012903:Smell / Q000502:physiology*; D014370:Tsetse Flies / Q000502:physiology*; D014835:Volatilization
D000442:Octanols; D010675:Pheromones; D010938:Plant Oils; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; C010411:insect attractants; C024714:caryophyllene; C038844:1-octen-3-ol