[Effects of E1A gene on the growth and chemosensitivity of transplantation tumor of nude mice].
OBJECTIVE
To study the effects of E1A gene on the growth and chemosensitivity of transplantation tumor of nude mice of lymph node metastasis cell line (686LN-1) of human tongue squamous cell carcinoma.


METHODS
686LN-1, 686LN-1-vect and 686LN-1-E1A cells were transplanted into nude mice, then the time of tumor formation, growth rates and weight of tumor were observed. To study the effects of the E1A gene on bleomycin sensitivity in vivo, 686LN-1 cells were injected into nude mice. After tumor formed, bleomycin, E1A gene and E1A gene + bleomycin were given respectively as therapy. The tumor volume was calculated, and growth curve was plotted. Representative histological sections were taken from mice bearing transplantation tumor either treated or control groups, and expression of HER-2/neu was detected.


RESULTS
In nude mice, the expression of E1A gene significantly suppressed the growth rates and elongated the time of tumor formation. Bleomycin or E1A Gene and E1A gene + bleomycin can suppress the growth rates of transplantation tumor of nude mice, the suppressed rates of tumor growth was 53.13%, 76.83% and 96.65%, respectively. The expression of E1A gene can significantly suppressed the expression of HER-2/neu gene in 686LN-1-E1A cells.


CONCLUSIONS
E1A is able to significantly inhibit the growth rate of transplantation tumor of nude mice of lymph node metastasis cell line (686LN-1) of human tongue squamous cell carcinoma, and dramatically enhance the sensitivity of the cells to cytotoxic agents in vivo. Above of all functions of E1A gene may be related to that it suppressed the expression of HER-2/neu gene.
Wang
Xiao-lei
XL
Department of Head and Neck Surgery, Cancer Hospital, Chinese Academy of Medical Sciences, Beijing 100021, China. doctorwangxiaolei@263.net
Qian
Xin-lai
XL
Zhao
Qing-zheng
QZ
Xu
Zhen-gang
ZG
Tang
Ping-zhang
PZ
D017500:Adenovirus E1A Proteins / Q000235:genetics*; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D001761:Bleomycin / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D018734:Genes, erbB-2 / Q000235:genetics; D015316:Genetic Therapy; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis / Q000235:genetics; D014062:Tongue Neoplasms / Q000473:pathology*
D017500:Adenovirus E1A Proteins; D000903:Antibiotics, Antineoplastic; D001761:Bleomycin; D018719:Erb-b2 Receptor Tyrosine Kinases