title: Myofibroblast numbers are elevated in human elbow capsules after trauma.
abstract: Elbow contractures, a frequent problem after injury, can be treated by excision of the joint capsule. However, the underlying changes in the joint capsule are poorly understood. Based on skin healing work, we examined the hypotheses that myofibroblast numbers and expression of a myofibroblast marker alpha-smooth muscle actin, are elevated in patients with posttraumatic joint contractures. Anterior capsules were obtained from six patients who had operative release of posttraumatic contractures greater than 5 months after injury and six elbows of organ donors free of contractures. Immunohistochemical studies revealed that myofibroblast numbers and percentage of total cells that were myofibroblasts were significantly elevated in the joint capsules from patients with contractures (326 +/- 61 cells per field, 36% +/- 4% total cells) when compared with similar tissues of the organ donors (69 +/- 41 cells per field, 9% +/- 4% total cells). Western blot analysis showed that protein levels of alpha-smooth muscle actin were significantly elevated in patients with posttraumatic joint contractures. However, analysis with reverse transcription-polymerase chain reaction determined that messenger ribonucleic acid levels for smooth muscle actin normalized to the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase were not significantly different between the two groups. An association between increased numbers of myofibroblasts and posttraumatic joint contractures has been established in the human elbow capsule. Additional work is required to determine whether myofibroblast regulators may be targets for adjuvant therapies of posttraumatic contractures.
authors.affiliation: McCaig Centre for Joint Injury and Arthritis Research, University of Calgary, Canada. hildebrk@ucalgary.ca
authors.forename: Kevin A
authors.initials: KA
authors.lastname: Hildebrand
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
authors.forename: Wistara
authors.initials: W
authors.lastname: van Snellenberg
authors.forename: Graham J W
authors.initials: GJ
authors.lastname: King
authors.forename: David A
authors.initials: DA
authors.lastname: Hart
mesh_terms: D000328:Adult; D001707:Biopsy, Needle; D015153:Blotting, Western; D016022:Case-Control Studies; D002465:Cell Movement; D003286:Contracture / Q000209:etiology / Q000473:pathology*; D046508:Culture Techniques; D005260:Female; D005347:Fibroblasts / Q000473:pathology / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D017746:Joint Capsule / Q000166:cytology / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009130:Muscle, Smooth / Q000166:cytology / Q000502:physiology*; D011336:Probability; D012016:Reference Values; D012044:Regression Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018570:Risk Assessment; D000092464:Elbow Injuries