Interaction of spermine with a signal transduction pathway involving phospholipase C, during the growth of Catharanthus roseus transformed roots.
In Cantharanthus roseus transformed roots, the application of methylglyoxal bis(guanylhydrazone) (MGBG), an inhibitor of S-adenosylmethionine decarboxylase (SAMDC; EC 4.1.1.50), inhibited the root growth in a dose-dependent manner with a DL(50) of about 300 micro m. Spermidine and spermine (Spm) levels and SAMDC and phospholipase C (PLC; EC 3.1.4.3) activities were reduced in the presence of the inhibitor. The inhibition was reversed by the addition of Spm. Radioactivity from [(14)C]Spm was detected in an immunoprecipitated fraction with an antibody anti-PLC-delta. To our knowledge, this is the first direct evidence that demonstrates an interaction of Spm with the signal transduction cascade phosphoinositide-Ca(2+).
Echevarría-Machado
Ileana
I
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán. Calle 43 #130, Colonia Chuburná de Hidalgo. c.p 97200, Mérida, Yucatán, México.
Ku-González
Angela
A
Loyola-Vargas
Victor M.
VM
Hernández-Sotomayor
S. M. Teresa
SM