title: Investigation of gamma-irradiated vegetable seeds with high-resolution solid-state 13C NMR.
abstract: 13C solid-state NMR was used to investigate the effects of gamma radiation on vegetable seeds, Pisum sativum and Latuca sativa, at absorbed doses that inhibit their germination. By combining single-pulse excitation and cross-polarization experiments under magic angle spinning, both liquid and solid domains of seeds can be characterized. We showed that the liquid domains, mostly made of triacylglycerols (TAG), of vegetable seeds are not sensitive to radiation. The main structural changes have been observed in the embryonic axes of seeds when the seeds are water-imbibed before irradiation. These results rule out a starting hypothesis concerning the potential role of TAG contained in oil bodies as a potential source of aldehydes that could further react with DNA moiety.
authors.affiliation: Département de Recherche Fondamentale sur la Matière Condensée-Service de Chimie Inorganique et Biologique CEA-Grenoble, F-38054 Grenoble Cedex 9, France. bardet@drfmc.ceng.cea.fr
authors.forename: Michel
authors.initials: M
authors.lastname: Bardet
authors.forename: Sébastien
authors.initials: S
authors.lastname: Maron
authors.forename: Marie Françoise
authors.initials: MF
authors.lastname: Foray
authors.forename: Maurice
authors.initials: M
authors.lastname: Berger
authors.forename: Armel
authors.initials: A
authors.lastname: Guillermo
mesh_terms: D004247:DNA / Q000528:radiation effects; D005720:Gamma Rays; D018545:Lactuca / Q000528:radiation effects; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D018532:Pisum sativum / Q000528:radiation effects; D012639:Seeds / Q000528:radiation effects*; D013053:Spectrophotometry; D014280:Triglycerides / Q000737:chemistry; D014675:Vegetables / Q000528:radiation effects*
chemical_list: D014280:Triglycerides; D004247:DNA