Yania halotolerans gen. nov., sp. nov., a novel member of the suborder Micrococcineae from saline soil in China.
A novel coccoid, halotolerant actinobacterium, designated strain YIM 70085(T), was isolated from a soil sample that was collected in Xinjiang Province, China, and characterized by using a polyphasic approach. Optimum growth temperature was 28 degrees C and growth occurred optimally in culture media that contained 10 % KCl. The peptidoglycan type was A4alpha, L-lys-gly-L-Glu. Whole-cell sugars consisted of xylose, mannose and galactose. Phospholipids were diphosphatidylglycerol, phosphatidylglycerol, one unknown phospholipid, one unknown glycolipid and traces of phosphatidylinositol. Menaquinones were MK-8 (83 %), MK-7 (12 %) and MK-9 (15 %). Predominant fatty acids were i-C(15 : 0) (44.29 %), ai-C(15 : 0) (35.60 %) and ai-C(17 : 0) (9.74 %). The DNA G+C content was 53.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 70085(T) occupies a branch that is distinct from, although very close to, the family Micrococcaceae in the suborder Micrococcineae. Based on its phenotypic characteristics, phylogenetic position (as determined by 16S rRNA gene sequence analysis) and 16S rDNA signature nucleotide data, it is concluded that the isolate represents a novel member of the suborder Micrococcineae, for which the name Yania halotolerans gen. nov., sp. nov. is proposed. The type strain is YIM 70085(T) (=CCTCC AA001023(T)=DSM 15476(T)).
Li
Wen-Jun
WJ
Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P.R. China.
Chen
Hua-Hong
HH
Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P.R. China.
Xu
Ping
P
Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P.R. China.
Zhang
Yu-Qin
YQ
Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P.R. China.
Schumann
Peter
P
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
Tang
Shu-Kun
SK
Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P.R. China.
Xu
Li-Hua
LH
Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P.R. China.
Jiang
Cheng-Lin
CL
Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P.R. China.
D001482:Base Composition; D001483:Base Sequence; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D008835:Micrococcaceae / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D012988:Soil Microbiology; D013696:Temperature
D004269:DNA, Bacterial