Characterization of a region involved in binding of measles virus H protein and its receptor SLAM (CD150).
Signaling lymphocyte activation molecule (SLAM; also known as CD150) is a newly identified cellular receptor for measles virus (MV). MV Hemagglutinin protein (H) mediates MV entry into host cells by specifically binding to SLAM. Amino acid 27-135 of SLAM was previously shown to be the functional domain to interact with H and used to screen a 10-mer phage display peptide library in this study. After four rounds of screening and sequence analysis, the deduced amino acid sequence of screened peptides SGFDPLITHA and SDWDPLFTHK showed to be highly homologous with amino acid 429-438 of MV H (SGFGPLITHG). Peptides SGFDPLITHA and SDWDPLFTHK specifically inhibited binding of H to SLAM and further inhibition of MV infection suggests that these peptides can be developed to MV blocking reagents and amino acid 429-438 in H protein is functionally involved in receptor binding and may constitute part of the receptor-binding determinants on H protein.
Hu
Chunling
C
Institute of Virology, College of Life Science, Wuhan University, Wuhan 430072, PR China.
Zhang
Peng
P
Liu
Xin
X
Qi
Yipeng
Y
Zou
Tingting
T
Xu
Qin
Q
D000818:Animals; D015703:Antigens, CD; D001665:Binding Sites; D016466:CHO Cells; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis*; D007136:Immunoglobulins / Q000096:biosynthesis*; D007150:Immunohistochemistry; D008459:Measles virus / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011956:Receptors, Cell Surface; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D014162:Transfection; D014709:Vero Cells; D014764:Viral Proteins / Q000737:chemistry* / Q000378:metabolism
D015703:Antigens, CD; D006023:Glycoproteins; D007136:Immunoglobulins; D019151:Peptide Library; D010455:Peptides; D011956:Receptors, Cell Surface; D014764:Viral Proteins; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1