[Antigen expression and the endothelization of biologic blood vessel matrix].
This study sought to explore the change of the major histocompatibility complex (MHC) antigen expression and the endothelization of blood vessel in minor pig after trypsin treatment, and to provide data for xenotransplantation and pig vessel for use in tissue engineering. Western blot assays were conducted for detecting the expression of MHC xenoantigens. Scanning electron microscopy was used for checking the endothelization of decellularized blood vessel. The results showed that MHC antigen is not expressed after trypsin treatment. The endothelization is accomplished. The endothelial cells have normal morphological distribution. These demonstrate that the antigen of pig aorta is significantly decreased and it can be used for constructing new vascular grafts.
Zhu
Chuhong
C
Biomechanics Section, Key Lab for Biomechanics & Tissue Engineering of Ministry of Education, Department of Anatomy, Third Military Medical University, Chongqing 400038, China.
Ying
Dajun
D
Mi
Jianhong
J
Sun
Jiansen
J
Zhang
Wei
W
D000818:Animals; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology; D005260:Female; D008285:Major Histocompatibility Complex / Q000502:physiology*; D008297:Male; D013552:Swine; D013556:Swine, Miniature; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds