title: Improved photostability and fluorescence properties through polyfluorination of a cyanine dye.
abstract: [structure: see text] A polyfluorinated cyanine dye has been synthesized and characterized. Compared with the nonfluorinated analogue, the dye exhibits significantly reduced aggregation in aqueous media, enhanced fluorescence quantum yield, greater resistance to photobleaching upon direct irradiation, and reduced reactivity toward singlet oxygen. All of these properties are favorable for use of cyanine dyes as fluorescent labels and point toward fluorination as a general strategy for improving performance in imaging applications.
authors.affiliation: Department of Chemistry, Molecular Biosensor and Imaging Center, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213-3890, USA.
authors.forename: Babu Rao
authors.initials: BR
authors.lastname: Renikuntla
authors.forename: Heather C
authors.initials: HC
authors.lastname: Rose
authors.forename: Joby
authors.initials: J
authors.lastname: Eldo
authors.forename: Alan S
authors.initials: AS
authors.lastname: Waggoner
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Armitage
mesh_terms: D002232:Carbocyanines / Q000138:chemical synthesis / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D005461:Fluorine / Q000737:chemistry*; D008027:Light; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D038761:Photobleaching; D026082:Singlet Oxygen / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; D026082:Singlet Oxygen; D005461:Fluorine