title: [Construction and identification of yeast expression vectors containing human platelet-derived growth factor B-chain gene].
abstract: OBJECTIVE
  To investigate the expression efficiency of human platelet-derived growth factor B-chain (PDGF-B) gene in yeast and assess the activity of the expressed product.
  
  
  METHODS
  A full-length complementary DNA of human PDGF-B gene was amplified from the total RNA extracted from human vascular endothelial cells using reverse transcription (RT)-PCR and then cloned into pGEM-T vector. The PCR products with specific primers were recombined into yeast expression plasmids pMETB or pMETalphaA, followed by identification with restriction endonuclease.
  
  
  RESULTS
  The 578 bp fragment encoding mature PDGF-BB peptide with signal peptide and the 340 bp fragment without signal peptide were identified and verified by restriction endonuclease mapping and sequencing, and two yeast expression vectors pMETB-PDGFB(1) and pMETalphaA-PDGFB(2) were reconstructed.
  
  
  CONCLUSIONS
  The yeast expression vectors containing human PDGF-B gene have been successfully constructed for further studies of gene expression in yeast.
authors.affiliation: Department of Orthopedic Surgery, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. ycl_2002@163.com
authors.forename: Chun-lu
authors.initials: CL
authors.lastname: Yang
authors.forename: Jian-ting
authors.initials: JT
authors.lastname: Chen
authors.forename: Fan
authors.initials: F
authors.lastname: Deng
authors.forename: Huan-zhang
authors.initials: HZ
authors.lastname: Tang
authors.forename: Xiao-yun
authors.initials: XY
authors.lastname: Tan
authors.forename: Shen-qiu
authors.initials: SQ
authors.lastname: Luo
mesh_terms: D000077214:Becaplermin; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D020574:Proto-Oncogene Proteins c-sis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015003:Yeasts / Q000235:genetics*
chemical_list: D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin