title: [Effects of musk glucoprotein on chemotaxis of polymorphonuclear leukocytes in vivo and in vitro].
abstract: OBJECTIVE
  To investigate the effects of Musk glucoprotein on chemotaxis of Polymorphonuclear leukocytes(PMN).
  
  
  METHODS
  The chemotaxis of PMN in abdominal cavity in rat induced by carboxymethyl cellulose(CMC) was used as an in vivo animal model and in in vitro it was evaluated by Boyden chamber. The concentration of cytosolic free Ca2+ was quantitated with the fluorescent Ca2+ indicator Fura-2.
  
  
  RESULTS
  The water extract of Musk at dose of 5, 20, 80 mg.kg-1 (s.c.) significantly inhibited the chemotaxis of PMN in rat; Musk-1 at concentration of 1-100 micrograms.mL-1 can significantly inhibit the chemotaxis of rabbit PMN in vitro; Musk-1 at concentration of 1-100 micrograms.mL-1 can significantly inhibit the increase of cytosolic Ca2+ concentration in PMN of rat.
  
  
  CONCLUSIONS
  Part of mechanisms underlying antiinflammatory action of Musk is to inhibit the chemotaxis of PMN.
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences, Chinese Peiking Union Medical College, Beijing 100050. wwenj@imm.ac.cn
authors.forename: Wen-jie
authors.initials: WJ
authors.lastname: Wang
authors.forename: Miao
authors.initials: M
authors.lastname: Zhong
authors.forename: Ying
authors.initials: Y
authors.lastname: Guo
authors.forename: Long-en
authors.initials: LE
authors.lastname: Zhou
authors.forename: Gui-fang
authors.initials: GF
authors.lastname: Cheng
authors.forename: Xiu-yuan
authors.initials: XY
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D006023:Glycoproteins / Q000302:isolation & purification / Q000494:pharmacology; D008297:Male; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D009504:Neutrophils / Q000378:metabolism / Q000502:physiology*; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D005229:Fatty Acids, Monounsaturated; D006023:Glycoproteins; D008419:Materia Medica; C008563:musk; D002118:Calcium