DNA modification by oxovanadium(IV) complexes of salen derivatives.
Oxovanadium(IV) complexes of hydroxysalen derivatives have been prepared and tested as DNA reactive agents. The nuclease activity has been investigated under oxidative or reducing conditions, on the basis of the various oxidation states of vanadium: V(III), V(IV) and V(V). In the absence of an activating agent, none of the compounds tested was able to induce cleavage of DNA, whereas in the presence of mercaptopropionic acid (MPA) or Oxone the four complexes induced DNA modifications. Under both conditions, the para-hydroxy complex was found to be the most active compound. Reaction of these salen complexes with DNA occurs essentially at guanine residues and is more efficient in the presence of Oxone than under reducing conditions. The extent of Oxone-mediated DNA oxidation by the four vanadyl complexes was clearly superior to VOSO(4) and was observed without piperidine treatment. EPR studies provided information on the reactive metal-oxo species involved under each conditions and a mechanism of reaction with DNA is discussed.
Verquin
Géraldine
G
Laboratoire de Chimie Organique et Macromoléculaire, UMR CNRS 8009, USTL Bât. C3, 59655, Villeneuve d'Ascq, France.
Fontaine
Gaëlle
G
Bria
Marc
M
Zhilinskaya
Elena
E
Abi-Aad
Edmond
E
Aboukaïs
Antoine
A
Baldeyrou
Brigitte
B
Bailly
Christian
C
Bernier
Jean-Luc
JL
D004278:DNA, Superhelical / Q000737:chemistry* / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D005029:Ethylenediamines / Q000737:chemistry* / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D019097:Spin Trapping / Q000379:methods; D013464:Sulfuric Acids / Q000737:chemistry; D014638:Vanadates / Q000737:chemistry* / Q000378:metabolism*
D004278:DNA, Superhelical; D005029:Ethylenediamines; D013464:Sulfuric Acids; C075743:oxovanadium IV; D014638:Vanadates; C011452:disalicylaldehyde ethylenediamine; C048813:potassium peroxymonosulfuric acid