Two ITS forms co-inhabiting a single genet of an isolate of Terfezia boudieri (Ascomycotina), a desert truffle.
Two fruit-bodies of Terfezia boudieri Chatin, each exhibiting a mixture of two ITS -RFLP profiles, were found in the Negev desert of Israel. A mycelial culture obtained from glebal out-growth maintained the double profile, as did proliferating cultures established using single hyphae isolated from the original cultures. The main difference between the two ITS variants lies in a 21 bp deletion in the smaller variant. The question whether both variants are contained within a single nucleus or occupy different nuclei sharing the same cytoplasm is discussed.
Aviram
Sharon
S
The Institutes for Applied Research, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
Roth-Bejerano
Nurit
N
Kagan-Zur
Varda
V
D001203:Ascomycota / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D030601:Cistaceae / Q000382:microbiology*; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification; D021901:DNA, Intergenic / Q000235:genetics*; D003889:Desert Climate; D025301:Hyphae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D007557:Israel; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017384:Sequence Deletion
D004271:DNA, Fungal; D021901:DNA, Intergenic