Senescence-associated decline of lymphocyte migration in gut-associated lymphoid tissues of rat small intestine.
Scenescence-induced changes in gut-associated lymphoid tissues may contribute largely to the impaired immune responses during aging. Age-related changes in lymphocyte recirculations were investigated in Peyer's patches of rat small intestine. Cell dynamics of labeled T lymphocytes were observed under an intravital fluorescence microscope and compared between young and aged rats. Lymphocyte transport through intestinal lymph was decreased in aged rats. The lymphocyte rolling and adherence in postcapillary venules (PCV) of Peyer's patches was also significantly impaired in aged rats, with decreased expression of L-selectin on lymphocyte surfaces. Immunohistochemical analysis revealed a significant decrease in the CD8-positive cell population in Peyer's patches of the aged group, although mucosal addressin cell adhesion molecule-1 (MAdCAM-1) expression in postcapillary venules was unaltered. Lymphocyte adoptive-transfer studies indicated that although both the donor and recipient factors influence the adherence of T cells, the former may play a predominant role in the age-related change. This study clearly demonstrated in situ that T cell migration into Peyer's patches is significantly decreased in the aged intestine, which may reflect the impaired immune responses in the aging process.
Ogino
Takashi
T
Department of Internal Medicine, School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan. t-ogino@nx.catv.ne.jp
Miura
Soichiro
S
Komoto
Shunsuke
S
Hara
Yuriko
Y
Hokari
Ryota
R
Tsuzuki
Yoshikazu
Y
Watanabe
Chikako
C
Koseki
Seiichiro
S
Nagata
Hiroshi
H
Hachimura
Satoshi
S
Kaminogawa
Shuichi
S
Ishii
Hiromasa
H
D019264:Adoptive Transfer; D000375:Aging / Q000276:immunology*; D000818:Animals; D002465:Cell Movement / Q000276:immunology*; D004727:Endothelium / Q000378:metabolism; D007136:Immunoglobulins / Q000378:metabolism; D039441:Integrin alpha4 / Q000378:metabolism; D007421:Intestine, Small / Q000276:immunology; D019041:L-Selectin / Q000378:metabolism; D008297:Male; D009088:Mucoproteins / Q000378:metabolism; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology*; D051381:Rats; D011916:Rats, Inbred F344; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000378:metabolism
D007136:Immunoglobulins; C504268:Madcam1 protein, rat; D009088:Mucoproteins; D019041:L-Selectin; D039441:Integrin alpha4