Streptococcus minor sp. nov., from faecal samples and tonsils of domestic animals.
Nine isolates, which were obtained from tonsils, anal swabs and faeces of dogs and from tonsils of a cat and a calf, constituted a homogeneous but unidentified taxon after screening with tRNA intergenic length polymorphism analysis and whole-cell protein fingerprinting. 16S rDNA sequence analysis classified representative strains in the genus Streptococcus. Highest sequence similarity (95.9 %) was obtained with Streptococcus ovis. Growth characteristics, biochemical features, DNA-DNA hybridization and DNA G+C contents of selected strains demonstrated that they represent a single, novel streptococcal species. The name Streptococcus minor sp. nov. is proposed for the novel species; the type strain (ON59(T)=LMG 21734(T)=CCUG 47487(T)) was isolated from a dog tonsil.
Vancanneyt
M
M
BCCM/LMG Bacteria Collection, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B 9000 Ghent, Belgium.
Devriese
L A
LA
Laboratory of Veterinary Bacteriology and Mycology, Ghent University, Salisburylaan 133, B 9820 Merelbeke, Belgium.
De Graef
E M
EM
Laboratory of Veterinary Bacteriology and Mycology, Ghent University, Salisburylaan 133, B 9820 Merelbeke, Belgium.
Baele
M
M
Laboratory of Veterinary Bacteriology and Mycology, Ghent University, Salisburylaan 133, B 9820 Merelbeke, Belgium.
Lefebvre
K
K
BCCM/LMG Bacteria Collection, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B 9000 Ghent, Belgium.
Snauwaert
C
C
BCCM/LMG Bacteria Collection, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B 9000 Ghent, Belgium.
Vandamme
P
P
Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B 9000 Ghent, Belgium.
Swings
J
J
Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B 9000 Ghent, Belgium.|BCCM/LMG Bacteria Collection, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B 9000 Ghent, Belgium.
Haesebrouck
F
F
Laboratory of Veterinary Bacteriology and Mycology, Ghent University, Salisburylaan 133, B 9820 Merelbeke, Belgium.
D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology*; D001482:Base Composition; D002415:Cats; D002417:Cattle; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004285:Dogs; D005243:Feces / Q000382:microbiology*; D008969:Molecular Sequence Data; D014066:Palatine Tonsil / Q000382:microbiology*; D010802:Phylogeny; D013291:Streptococcus / Q000145:classification* / Q000302:isolation & purification*
D004269:DNA, Bacterial