[Determination of glycosides in traditional Chinese medicine liu-wei di-huang by RP-HPLC].
OBJECTIVE
To establish a RP-HPLC method for determination of glycosides in Traditional Chinese Medicine Liu-wei Di-huang.


METHODS
The samples were analyzed on an ODS column at 30 degrees C, with mobile phase of methanol/water (33:67) at flow rate 1.0 mL.min and detection at wavelength of 236 nm.


RESULTS
Three major components reached base-line separation and were identified to be mononiside, loganin, paeoniflorin. Respectively for the three components, linear correlations were found between peak areas and concentrations in the ranges of 7.4-60, 7.7-62 mg.L-1 and 8.5-68 mg.L-1, and the recoveries were 98.8%, 98.3%, 99.6%.


CONCLUSIONS
The established method is proved to be suitable for simultaneous quantification of three major glycosidic components in Liuwei Dihuang decoction and can be used for evaluation of the quality of Liuwei Dihuang preparations.
Xiong
Shan-li
SL
Academy of Military Medical Sciences, Institute of Pharmacology and Toxicology, Bingjing 100850, China.
Zhao
Yi-min
YM
Luan
Xin-hui
XH
Qiao
Shan-yi
SY
Sun
Lei
L
Ren
Feng-xia
FX
Guo
Ji-fen
JF
Zhang
Yong-xiang
YX
D001565:Benzoates / Q000032:analysis*; D001952:Bridged-Ring Compounds / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D029768:Cornus / Q000737:chemistry; D027742:Dioscorea / Q000737:chemistry; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis*; D039823:Iridoids / Q000032:analysis*; D039821:Monoterpenes; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D032267:Rehmannia / Q000737:chemistry; D006027:Glycosides
D001565:Benzoates; D001952:Bridged-Ring Compounds; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039823:Iridoids; D039821:Monoterpenes; C042579:Liuwei Dihuang Decoction; C015423:peoniflorin; D006027:Glycosides; C059516:loganin