title: Comparison of pulsed field gel electrophoresis patterns of Bordetella pertussis isolates from unvaccinated children with severe or mild pertussis.
abstract: BACKGROUND
  The clinical spectrum of pertussis ranges from mild cough illnesses of short duration to typical illness with paroxysmal cough, whooping and posttussive vomiting. We performed comparative genotyping of Bordetella pertussis isolates from children in Germany with severe or mild pertussis.
  
  
  METHODS
  Pulsed field gel electrophoresis (PFGE) of genomic DNA, digested with the restriction endonucleases Xba I and Spe I, was used to determine the profiles of strains isolated from 31 unvaccinated children with severe symptoms of pertussis (with hospitalization and/or complications) compared with isolates from 32 matched children with mild symptoms.
  
  
  RESULTS
  No significant differences in PFGE patterns were found in B. pertussis strains isolated from children with severe vs. mild illness.
  
  
  CONCLUSIONS
  Differences in virulence of circulating B. pertussis strains can be absent or might not correlate with specific PFGE patterns. Other methods should be evaluated to identify differences in virulence.
authors.affiliation: University Hospital for Children and Adolescents, Erlangen, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Heininger
authors.forename: Reno
authors.initials: R
authors.lastname: Frei
authors.forename: James D
authors.initials: JD
authors.lastname: Cherry
authors.forename: Klemens
authors.initials: K
authors.lastname: Stehr
mesh_terms: D001886:Bordetella pertussis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D011795:Surveys and Questionnaires; D014917:Whooping Cough / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial