title: [Study on plant tissue culture of Cornus officinalis].
abstract: OBJECTIVE
  To set up the optimums of tissue culture for Cornus officinalis.
  
  
  METHODS
  A section of stems of the fine varieties of Cornus officinalis were strictly sterilized, cultivated and geminated in medium with addition of different kinds of hormone.
  
  
  CONCLUSIONS
  The WPM medium with 6-BA 2.0 mg.L-1 + ZT 0.1 mg.L-1 + NAA 0.1 mg.L-1 was the optimum medium for the multiplication of adventitious buds. The WPM medium with 6-BA 1.0 mg.L-1 + ZT 0.1 mg.L-1 + GA3 0.5 mg.L-1 + NAA 0.1 mg.L-1 was the optimum medium for cultivation in strength. The plantlet could root well in 1/2 MS medium with IBA 1.0 mg.L-1 + 6-BA 0.1 mg.L-1.
authors.affiliation: Department of Biology, Huaibei Coal Industry Teacher's College, Huaibei 235000, Anhui, China. xuejp2000@yahoo.com.cn
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Xue
authors.forename: Ai-min
authors.initials: AM
authors.lastname: Zhang
authors.forename: Yue-hui
authors.initials: YH
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Sheng
mesh_terms: D029768:Cornus / Q000254:growth & development*; D003470:Culture Media; D046508:Culture Techniques; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000254:growth & development; D018547:Plant Stems / Q000254:growth & development; D010946:Plants, Medicinal / Q000254:growth & development*
chemical_list: D003470:Culture Media; D010937:Plant Growth Regulators