title: Tissue-engineered human asthmatic bronchial equivalents.
abstract: The isolation of human bronchial epithelial (HBEC) and fibroblastic cells (HBFC) from biopsies of asthmatic and non-asthmatic volunteers provided unique cellular materials to be used for the production of bioengineered bronchial equivalents (BE) in vitro. The HBEC are grown on a mesenchymal layer seeded with HBFC and the BE can be maintained for at least 15 days in culture. Under the BE culture conditions established previously, HBEC undergo differentiation into ciliated and goblet cells, within a pseudostratified organization comparable to human bronchi. We published previously the results from histologic and functional analyses of such BE produced exclusively using non-asthmatic HBEC and HBFC. We report here the comparative analyses of BE produced with non-asthmatic and asthmatic living HBEC and HBFC (naBE and aBE, respectively). Our data indicated that all asthmatic HBEC populations grown on a mesenchymal layer, containing non-asthmatic HBFC, slowly reached a confluent state but then detached from the matrix upon culture time. These BE appear to be very good models to study the mechanisms involved in asthma in vitro.
authors.affiliation: Laboratoire de Génie Tissulaire/LOEX, Hôpital de l'Enfant-Jésus du CHA, Québec, Canada.
authors.forename: Jean-Sébastien
authors.initials: JS
authors.lastname: Paquette
authors.forename: Véronique
authors.initials: V
authors.lastname: Moulin
authors.forename: Pierrot
authors.initials: P
authors.lastname: Tremblay
authors.forename: Vincent
authors.initials: V
authors.lastname: Bernier
authors.forename: Michel
authors.initials: M
authors.lastname: Boutet
authors.forename: Michel
authors.initials: M
authors.lastname: Laviolette
authors.forename: François A
authors.initials: FA
authors.lastname: Auger
authors.forename: Louis-Philippe
authors.initials: LP
authors.lastname: Boulet
authors.forename: Francine
authors.initials: F
authors.lastname: Goulet
mesh_terms: D000328:Adult; D000818:Animals; D001249:Asthma / Q000473:pathology*; D001706:Biopsy; D001980:Bronchi / Q000166:cytology* / Q000473:pathology; D002478:Cells, Cultured / Q000187:drug effects / Q000201:enzymology; D002923:Cilia / Q000648:ultrastructure; D003470:Culture Media / Q000494:pharmacology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D046508:Culture Techniques / Q000379:methods; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology* / Q000201:enzymology; D005347:Fibroblasts / Q000166:cytology; D018093:Gelatinases / Q000378:metabolism; D006801:Humans; D008648:Mesoderm; D008854:Microscopy, Electron; D008875:Middle Aged; D051381:Rats; D023822:Tissue Engineering / Q000379:methods
chemical_list: D003470:Culture Media; D017077:Culture Media, Conditioned; D018093:Gelatinases