title: Detection by real-time PCR of the Pfcrt T76 mutation, a molecular marker of chloroquine-resistant Plasmodium falciparum strains.
abstract: An efficient and fast diagnostic assay was developed to detect the point mutation involved in the resistance of Plasmodium falciparum to chloroquine. The test, based upon a real-time PCR principle and performed with the LightCycler system, was carried out on venous blood isolates from 221 cases of falciparum malaria. The assay provided a quick, sensitive and reliable detection of the T76 Pfcrt mutation and the chloroquine resistance. The results of this study suggest that this LightCycler-based technique could be used to optimise the treatment of imported uncomplicated falciparum malaria, and also for the molecular surveillance of drug resistance among imported cases of this disease.
authors.affiliation: Service de Parasitologie, Molecular Diagnosis Unit, Centre Hospitalier Universitaire Rangueil, 31403 Toulouse 4, France.
authors.forename: Aurélia
authors.initials: A
authors.lastname: Vessière
authors.forename: Antoine
authors.initials: A
authors.lastname: Berry
authors.forename: Richard
authors.initials: R
authors.lastname: Fabre
authors.forename: Françoise
authors.initials: F
authors.lastname: Benoit-Vical
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Magnaval
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D002738:Chloroquine / Q000494:pharmacology*; D016054:DNA, Protozoan / Q000032:analysis; D004351:Drug Resistance / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D016778:Malaria, Falciparum / Q000175:diagnosis / Q000469:parasitology; D008565:Membrane Proteins / Q000235:genetics*; D026901:Membrane Transport Proteins; D021261:Parasitic Sensitivity Tests; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000302:isolation & purification; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D015800:Protozoan Proteins; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000962:Antimalarials; D016054:DNA, Protozoan; D005819:Genetic Markers; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C416369:PfCRT protein, Plasmodium falciparum; D015800:Protozoan Proteins; D002738:Chloroquine