title: Improved fractionation of glycinin and beta-conglycinin and partitioning of phytochemicals.
abstract: Glycinin-rich and beta-conglycinin-rich products are prepared by soy protein fractionation. Physicochemical characteristics of these proteins affect their unique, important functionality in food systems and industrial applications. Soybean isoflavones and saponins are phytochemicals with potential health benefits. Objectives of this protein fractionation research were to (1) improve protein and phytochemical extraction from defatted soy flakes and recovery in product fractions and (2) evaluate phytochemical partitioning and profile changes during fractionation. Extraction environments (pH, ethanol concentration, temperature, and water-to-flake ratio) were each varied during bench-scale optimization. Optimized conditions of 45 degrees C and 10:1 water-to-flake ratio were compared with previous conditions of 20 degrees C and 15:1 water-to-flake ratio and a soy protein isolate process at pilot scale. Optimized conditions yielded more beta-conglycinin with higher isoflavone and saponin concentrations, but fraction purity was diminished by glycinin contamination. Bench-scale data demonstrated that increased phytochemical extraction did not translate into increased concentrations in product fractions.
authors.affiliation: Department of Food Science and Human Nutrition and Center for Crops Utilization Research, Iowa State University, Ames, Iowa 50011-1061, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Rickert
authors.forename: Lawrence A
authors.initials: LA
authors.lastname: Johnson
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Murphy
mesh_terms: D052179:Antigens, Plant; D005591:Chemical Fractionation / Q000379:methods; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005916:Globulins / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007529:Isoflavones / Q000032:analysis; D012503:Saponins / Q000032:analysis; D055314:Seed Storage Proteins; D030262:Soybean Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D013696:Temperature
chemical_list: D052179:Antigens, Plant; D005916:Globulins; D007529:Isoflavones; D012503:Saponins; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; C011474:glycinin