In vitro maturation and transfer of equine oocytes after transport of ovaries at 12 or 22 degrees C.
Transportation of equine ovaries would allow shipment of oocytes for research purposes or transfer after the death of a valuable mare. The objective of this study was to compare two temperatures for maintaining ovaries during a transport interval of 18-24 h. The goal was to obtain pregnancies after transport of ovaries, maturation of oocytes in vitro, and transfer of oocytes. Each shipment was composed of ovaries four to seven mares collected from an abattoir. From each mare, one ovary was packaged at approximately 12 degrees C, and the other was packaged at approximately 22 degrees C. Upon arrival at our laboratory, oocytes were collected and cultured for 24 h. For each transfer, between 9 and 15 oocytes from each group were placed into the oviducts of estrous mares through standing flank laparotomies. Recipients received human chorionic gonadotropin (hCG; 2000 IU, i.v.) 30-36 h before transfer (to synchronize ovulation). Recipients were inseminated 18-20 h before transfers with 2 x 10(9) progressively motile sperm. Uteri of recipients were examined with ultrasound to determine the number of developing embryos. On Day 16 ( ovulation = day 0), developing embryos were recovered by uterine lavage. Parentage verification was performed on recovered vesicles. Pregnancy rates were analyzed by Chi-square. The percentage of oocytes that developed into embryonic vesicles on Day 16 was not different between transport temperatures (22 degrees C, 13/73, 18% versus 12 degrees C, 11/73, 15%). In conclusion, pregnancies were obtained from in vitro matured oocytes that were recovered from ovaries transported for 18-24h at 12 or 22 degrees C.
Preis
K A
KA
Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, CO 80523, USA.
Carnevale
E M
EM
Coutinho da Silva
M A
MA
Caracciolo di Brienza
V
V
Gomes
G M
GM
Maclellan
L J
LJ
Squires
E L
EL
D000818:Animals; D005260:Female; D006736:Horses; D018587:Oocyte Donation / Q000662:veterinary; D009865:Oocytes / Q000502:physiology*; D009926:Organ Preservation / Q000662:veterinary; D010053:Ovary / Q000166:cytology / Q000502:physiology*; D011247:Pregnancy; D013048:Specimen Handling / Q000379:methods / Q000662:veterinary*; D013696:Temperature; D013997:Time Factors