Membrane model for the G-protein-coupled receptor rhodopsin: hydrophobic interface and dynamical structure.
Rhodopsin is the only member of the pharmacologically important superfamily of G-protein-coupled receptors with a known structure at atomic resolution. A molecular dynamics model of rhodopsin in a POPC phospholipid bilayer was simulated for 15 ns, revealing a conformation significantly different from the recent crystal structures. The structure of the bilayer compared with a protein-free POPC control indicated hydrophobic matching with the nonpolar interface of the receptor, in agreement with deuterium NMR experiments. A new generalized molecular surface method, based on a three-dimensional Voronoi cell construction for atoms with different radii, was developed to quantify cross-sectional area profiles for the protein, lipid acyl chains and headgroups, and water. Thus, it was possible to investigate the bilayer deformation due to curvature of the individual lipid monolayers. Moreover, the generalized molecular surface derived hydrophobic interface allowed benchmarking of the hydropathy sequence analysis, an important structural genomics tool. Five water molecules diffused into internal hydration sites during the simulation, yielding a total of 12 internal waters. The cytoplasmic loops and the C-terminal tail, containing the G-protein recognition and protein sorting sequences, exhibited a high mobility, in marked contrast to the extracellular and transmembrane domains. The proposed functional coupling of the highly conserved ERY motif to the lipid-water interface via the cytoplasmic loops provides insight into lipid effects on G-protein-coupled receptor activation in terms of a flexible surface model, involving the spontaneous monolayer curvature.
Huber
Thomas
T
Laboratory of Molecular Biology and Biochemistry, Howard Hughes Medical Institute and Rockefeller University, New York, New York 10021, USA. hubert@mail.rockefeller.edu
Botelho
Ana V
AV
Beyer
Klaus
K
Brown
Michael F
MF
D000818:Animals; D003198:Computer Simulation; D019204:GTP-Binding Proteins / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D012243:Rhodopsin / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D008051:Lipid Bilayers; D014867:Water; D012243:Rhodopsin; D019204:GTP-Binding Proteins