[Determination of schisandrin in shengmaiyin by HPLC].
OBJECTIVE
To determine the concentration of schisandrin in Shengmaiyin with HPLC.


METHODS
The sample was extracted with ethyl acetate through supersonic wave. The solution was filtrated and evaporated. The residue was resolved with methanol and determined by HPLC using PHENOMENEX C18 (4.6 mm x 250 mm, 5 microns) chromatographic column, methanol-acetonitrile-water (15:15:10) as mobile phase. The wavelength for detection was 254 nm.


RESULTS
The peak of schizandrin appears on about 7.10 minutes. The standard curves of schizandrin were linear in the concentration range of 0.2-2.0 micrograms, r = 0.9996. The average recovery of schizandrin were 100.5% (RSD 2.84%).


CONCLUSIONS
This method was found to be sensitive, quick and accurate for the measurement of schizandrin concentrations in Shengmaiyin.
Wu
Xiao-feng
XF
Medical College of Chinese People's Armed Police Forces, Tianjin 300162, China.
Chen
Hong
H
Zhao
Zhi-ru
ZR
D000284:Administration, Oral; D002851:Chromatography, High Pressure Liquid; D034242:Cyclooctanes / Q000032:analysis*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D017705:Lignans / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011083:Polycyclic Compounds / Q000032:analysis*; D032141:Schisandra / Q000737:chemistry*
D034242:Cyclooctanes; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D017705:Lignans; D011083:Polycyclic Compounds; C034734:schizandrin A