title: Antioxidants and inhibitor of matrix metalloproteinase-1 expression from leaves of Zostera marina L.
abstract: In order to develop new anti-photoaging agents, we examined the antioxidative activity and the inhibition effect of matrix metalloproteinase-1 (MMP-1) on the extracts of a marine product, Zostera marina L., which is known for its potent activity. Three compounds (compounds 1, 2, and 3) were isolated from an ethyl acetate (EtOAc) soluble fraction of the product; they were identified as apigenin-7-O-beta-D-glucoside (1), chrysoeriol (2), and luteolin (3). These compounds were found to scavenge radicals and reactive oxygen species (ROS) and were measured to have SC50 values of 0.18 mM, 0.68 mM, and 0.01 mM against the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 0.04 mM, 0.03 mM, and 0.01 mM against the superoxide radical in the xanthine/xanthine oxidase system, respectively. Compound 3 suppressed the expression of MMP-1 by up to 44% at 4.0 microM and inhibited the production of interleukin 6 (IL-6), which is known as a cytokine that induces MMP-1 expression. From these results, compound 3 and the other compounds were determined to have antioxidative activity and to inhibit MMP-1 expression. Thus, the three compounds are expected to be useful for preventing the photoaging of skin.
authors.affiliation: R&D Center, Hanbul Cosmetic Corporation, 72-7 Yongsung-ri, Samsung-Myun, Umsung-Kun, Chungbuk 369-830, Korea. jiny@hanbul.co.kr
authors.forename: Jin Hui
authors.initials: JH
authors.lastname: Kim
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Cho
authors.forename: Sung Min
authors.initials: SM
authors.lastname: Park
authors.forename: Kyung Eun
authors.initials: KE
authors.lastname: Lee
authors.forename: Jeong Jae
authors.initials: JJ
authors.lastname: Lee
authors.forename: Bum Chun
authors.initials: BC
authors.lastname: Lee
authors.forename: Hyeong Bae
authors.initials: HB
authors.lastname: Pyo
authors.forename: Kyung Sik
authors.initials: KS
authors.lastname: Song
authors.forename: Hum Dai
authors.initials: HD
authors.lastname: Park
authors.forename: Yeo Pyo
authors.initials: YP
authors.lastname: Yun
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D001713:Biphenyl Compounds; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D061965:Matrix Metalloproteinase Inhibitors; D010851:Picrates / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D011480:Protease Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology*; D013481:Superoxides / Q000737:chemistry; D013778:Tetrazolium Salts; D013844:Thiazoles; D014466:Ultraviolet Rays; D035962:Zosteraceae / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016207:Cytokines; D061965:Matrix Metalloproteinase Inhibitors; D010851:Picrates; D011480:Protease Inhibitors; D013778:Tetrazolium Salts; D013844:Thiazoles; D013481:Superoxides; C004931:1,1-diphenyl-2-picrylhydrazyl; C022616:thiazolyl blue