title: Crystallization and preliminary X-ray analysis of the acyl carrier protein synthase (AcpS) from Staphylococcus aureus.
abstract: Acyl carrier protein synthase (AcpS) catalyzes the transfer of 4'-phophopantetheine from coenzyme A to the acyl carrier protein (ACP) to activate it for fatty-acid biosynthesis. Two crystal forms of Staphylococcus aureus AcpS have been generated at 277 K using either NaCl or PEG 6000 as a precipitant. The diffraction patterns of the crystals extend to 1.65 and 1.8 A, respectively. Full sets of X-ray diffraction data were collected from native crystals and the crystal structures were solved by molecular replacement.
authors.affiliation: Schering-Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, New Jersey 07033, USA.
authors.forename: Dayna L
authors.initials: DL
authors.lastname: Daubaras
authors.forename: Eileen M
authors.initials: EM
authors.lastname: Wilson
authors.forename: Todd
authors.initials: T
authors.lastname: Black
authors.forename: Corey
authors.initials: C
authors.lastname: Strickland
authors.forename: Brian M
authors.initials: BM
authors.lastname: Beyer
authors.forename: Peter
authors.initials: P
authors.lastname: Orth
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D012997:Solvents; D013211:Staphylococcus aureus / Q000201:enzymology*; D017855:Transferases (Other Substituted Phosphate Groups) / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D012997:Solvents; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D017855:Transferases (Other Substituted Phosphate Groups); C021364:holo-(acyl-carrier-protein) synthase