Cloning and characterization of an immunoglobulin A Fc receptor from cattle.
Here, we describe the cloning, sequencing and characterization of an immunoglobulin A (IgA) Fc receptor from cattle (bFcalphaR). By screening a translated EST database with the protein sequence of the human IgA Fc receptor (CD89) we identified a putative bovine homologue. Subsequent polymerase chain reaction (PCR) amplification confirmed that the identified full-length cDNA was expressed in bovine cells. COS-1 cells transfected with a plasmid containing the cloned cDNA bound to beads coated with either bovine or human IgA, but not to beads coated with bovine IgG2 or human IgG. The bFcalphaR cDNA is 873 nucleotides long and is predicted to encode a 269 amino-acid transmembrane glycoprotein composed of two immunoglobulin-like extracellular domains, a transmembrane region and a short cytoplasmic tail devoid of known signalling motifs. Genetically, bFcalphaR is more closely related to CD89, bFcgamma2R, NKp46, and the KIR and LILR gene families than to other FcRs. Moreover, the bFcalphaR gene maps to the bovine leucocyte receptor complex on chromosome 18. Identification of the bFcalphaR will aid in the understanding of IgA-FcalphaR interactions, and may facilitate the isolation of FcalphaR from other species.
Morton
H Craig
HC
Laboratory of Immunohistochemistry and Immunopathology (LIIPAT), Institute of Pathology, University of Oslo, Rikshospitalet, Oslo, Norway. craig.morton@labmed.uio.no
Pleass
Richard J
RJ
Storset
Anne K
AK
Dissen
Erik
E
Williams
John L
JL
Brandtzaeg
Per
P
Woof
Jenny M
JM
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D019556:COS Cells; D002417:Cattle / Q000276:immunology*; D002522:Chlorocebus aethiops; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008024:Ligands; D008969:Molecular Sequence Data; D010802:Phylogeny; D011961:Receptors, Fc / Q000235:genetics* / Q000276:immunology; D016415:Sequence Alignment; D013045:Species Specificity; D014162:Transfection
D015703:Antigens, CD; D018076:DNA, Complementary; C036832:Fc(alpha) receptor; D008024:Ligands; D011961:Receptors, Fc