title: Influence of thyroid hormone on mouse preantral follicle development in vitro.
abstract: OBJECTIVE
  To evaluate the role of 3,3',5-triiodothyronine (T(3)) on ovarian follicle development in vitro.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  University reproductive biology unit.
  
  
  METHODS
  CD1 mice (female).
  
  
  METHODS
  In vitro culture.
  
  
  METHODS
  Assessment of follicle and oocyte development following a 6-day culture with FSH (100 mU/mL), (Bu)(2)cAMP (0.5 mM), and T(3) (1-100 nM), alone or in combination.
  
  
  RESULTS
  A high percentage of in vitro grown (IVG) FSH-stimulated follicles formed an antral cavity (AC), while the addition of T(3) (100 nM) significantly reduced this response in a statistically significant manner. A statistically significant recovery of AC formation was obtained by limiting exposure to 4 days of culture. Formation of AC was induced by (Bu)(2)cAMP, which prevented T(3)-mediated suppression of AC formation. Under different conditions, high proportions of germinal vesicle-arrested IVG oocytes displayed condensed chromatin configuration. The capacity to undergo germinal vesicle breakdown (GVBD) was similar in all groups, and a statistically significant reduction in the percentage of oocytes with PB1 was recorded when T(3) was added to FSH or (Bu)(2)cAMP. This ability was partially recovered by removing T(3) on day 4 of culture.
  
  
  CONCLUSIONS
  Exposure to high T(3) concentrations can impair preantral follicle development, but this effect can be partly reverted by restoring a physiological hormonal milieu before follicle commitment to antral development.
authors.affiliation: Dipartimento di Scienze e Tecnologie Biomediche-L'Aquila, Via Vetoio 10, 67100 L'Aquila, Italy. cecconi@univaq.it
authors.forename: Sandra
authors.initials: S
authors.lastname: Cecconi
authors.forename: Gianna
authors.initials: G
authors.lastname: Rossi
authors.forename: Giovanni
authors.initials: G
authors.lastname: Coticchio
authors.forename: Guido
authors.initials: G
authors.lastname: Macchiarelli
authors.forename: Andrea
authors.initials: A
authors.lastname: Borini
authors.forename: Rita
authors.initials: R
authors.lastname: Canipari
mesh_terms: D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002417:Cattle / Q000097:blood / Q000196:embryology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002843:Chromatin / Q000187:drug effects / Q000648:ultrastructure; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005312:Fetal Blood; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D008540:Meiosis / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D009865:Oocytes / Q000166:cytology; D006080:Ovarian Follicle / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D014284:Triiodothyronine / Q000494:pharmacology*
chemical_list: D002843:Chromatin; D014284:Triiodothyronine; D004958:Estradiol; D003994:Bucladesine; D005640:Follicle Stimulating Hormone