Cancer chemopreventive effects of polyunsaturated fatty acids.
The inhibitory effects on Epstein-Barr virus early antigen (EBV-EA) activation induced by the tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA), in Raji cells as a primary screening test for anti-tumor promoters, for 22 fatty acids (as free and esterified forms), including 10 di- and polyunsaturated acids, and the inhibitory effects on activation of (+/-)-(E)-methtyl-2-[(E)-hydroxy-imino]-5-nitro-6-methoxy-3-hexemide (NOR 1), a nitric oxide (NO) donor, as a primary screening test for anti-tumor initiators, for 17 fatty acids (as methyl ester forms), were evaluated. Among the fatty acids tested, three n-3 polyunsaturated acids, eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA), exhibited potent inhibitory effects both on EBV-EA and NOR 1 activation. Furthermore, DHA methyl ester exhibited remarkable anti-tumor-promoting activity on an in vivo two-stage carcinogenesis test of mouse tumor using 7,12-dimethylbenz[a]anthracene (DMBA) as an initiator and TPA as a promoter.
Akihisa
Toshihiro
T
College of Science and Technology, Nihon University, 1-8 Kanda Surugadai, Chiyoda-ku, Tokyo 101-8308, Japan. akihisa@chem.cst.nihon-u.ac.jp
Tokuda
Harukuni
H
Ogata
Masakazu
M
Ukiya
Motohiko
M
Iizuka
Masao
M
Suzuki
Takashi
T
Metori
Koichi
K
Shimizu
Naoto
N
Nishino
Hoyoku
H
D000818:Animals; D000956:Antigens, Viral / Q000187:drug effects / Q000502:physiology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D051379:Mice; D009369:Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity
D000956:Antigens, Viral; C033305:Epstein-Barr virus early antigen; D005231:Fatty Acids, Unsaturated; D020030:Nitric Oxide Donors; D013755:Tetradecanoylphorbol Acetate