title: [Histo-morphological changes of eustachian tube of guinea pigs with effusion after being treated by pulmonary surfactant].
abstract: OBJECTIVE
  To study the effect of pulmonary surfactant on otitis media with effusion in guinea pigs to find a new way to manage otitis media with effusion.
  
  
  METHODS
  Nonviable heat-killed pneumococci (HKP) solution was inoculated into the middle ear cavity in guinea pigs via a transeardrum approach to set up a model of otitis media with effusion in guinea pigs. Seven days after being injected with pulmonary surfactant (PS) by transeardrum approach, ABR threshold and histomorphological changes of eustachian tube mucosa of guinea pigs were examined by light microscopy and scanning microscopy.
  
  
  RESULTS
  Five days following inoculation of HKB serous effusion were present in the middle ear cavity of guinea pigs, but disappearance of light cone. Response (mean +/- s) threshold raised from (14.0 +/- 3.1) dB to (45.0 +/- 5.7) dB. The eustachian tube mucosa was thickened and lined eosin-stained structureless matter over mucosa, while cilia of eustachian tube mucosa irregularly arranged. Seven days after being treated by PS, serous effusion of tympanum was reduced or disappeared, and response threshold decreased from (45.0 +/- 5.7) dB to (23.5 +/- 6.3) dB. There was significantly difference between them (P < 0.001). Eustachian tube mucosa was thinned, Cilia of eustachian tube mucosa regularly arranged to the nasopharynx.
  
  
  CONCLUSIONS
  Pulmonary surfactant plays a important role in otitis media with effusion of guinea pigs.
authors.affiliation: Department of Otorhinolaryngology, Yueyang Hospital, Traditional Chinese & Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China. Mazhaoxincn@yahoo.com.cn
authors.forename: Zhao-xin
authors.initials: ZX
authors.lastname: Ma
authors.forename: Xu-hui
authors.initials: XH
authors.lastname: Chen
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Huang
authors.forename: Yi
authors.initials: Y
authors.lastname: Cao
authors.forename: Jian-feng
authors.initials: JF
authors.lastname: Cai
mesh_terms: D000818:Animals; D005064:Eustachian Tube / Q000473:pathology*; D016057:Evoked Potentials, Auditory, Brain Stem; D005260:Female; D006168:Guinea Pigs; D008297:Male; D010034:Otitis Media with Effusion / Q000382:microbiology / Q000473:pathology* / Q000628:therapy; D011663:Pulmonary Surfactants / Q000494:pharmacology* / Q000627:therapeutic use; D011897:Random Allocation; D013296:Streptococcus pneumoniae
chemical_list: D011663:Pulmonary Surfactants