[The effect of bFGF on proliferation of periodontal fibroblast-like cells of dogs].
OBJECTIVE
To evaluate the effects of basic fibroblast growth factor(bFGF) on proliferation of periodontal fibroblast-like cells in vivo.


METHODS
A U-shaped osseous defect was produced on the buccal side of the mesial root. Four posterior teeth were conducted in four quadrants. Each quadrant included 4 groups: control, bFGF, expanded polytetrafluoroethylene(ePTFE) membrane, bFGF + ePTFE. Each time the 4 teeth sites in one quadrant were operated weekly and each dog experienced 4 times of operations. Bromodeoxyuridine(BrdU) was injected 1 hour prior to sacrificing the dogs at 4 weeks after first surgery. Immunohistochemical method was applied to count the BrdU-labeled fibroblast-like cells.


RESULTS
The number of BrdU-labeled cells reached the maximum at the 2nd week among all groups and then, decreased with time. Both bFGF and bFGF + ePTFE treated group had significantly more BrdU+ cells than remained control or ePTFE groups (P < 0.05) at 1st, 2nd weeks after surgery.


CONCLUSIONS
2 weeks after periodontal surgery is active phase of proliferation of periodontal fibroblasts. bFGF enhances fibroblast proliferation in early periodontal wound healing, and in turn accelerate periodontal regeneration.
Yang
Pi-shan
PS
Dept. of Periodontology, Affiliated Stomatological Hospital, Second Hospital, Shandong University, Jinan 250012, China.
Sun
Qin-feng
QF
Song
Ai-mei
AM
Ge
Shao-hua
SH
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004285:Dogs; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology*; D010519:Periodontium / Q000166:cytology* / Q000601:surgery; D012038:Regeneration; D014945:Wound Healing / Q000187:drug effects
D016222:Fibroblast Growth Factor 2