Mitogenic activity of Cratylia mollis lectin on human lymphocytes.
The mitogenic effect of Cratylia mollis seed lectin preparations containing two (Cramoll 1,4) or one molecular form (Cramoll 1) showed activity similar to the well known T-cell mitogen, concanavalin A (Con A). The effect on human lymphocytes was analyzed through a colorimetric assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). Inhibition of lymphocyte proliferation with methyl-alpha-d-mannoside (both preparations) indicated that the mitogenic effect involved carbohydrate lectin binding sites.
Maciel
Elba V M
EV
Universidade Federal de Pernambuco, Centro de Ciências Biológicas (CCB), Departamento de Bioquímica, Rua Prof. Nelson Chaves S/N, 50670-901 Cidade Universitária Recife-PE, Brazil.
Araújo-Filho
Vanduir S
VS
Nakazawa
Mineo
M
Gomes
Yara M
YM
Coelho
Luana C B B
LC
Correia
Maria T S
MT
D001665:Binding Sites; D002241:Carbohydrates / Q000737:chemistry; D002455:Cell Division; D004396:Coloring Agents / Q000494:pharmacology; D003208:Concanavalin A / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D010944:Plants / Q000378:metabolism*; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013997:Time Factors
D002241:Carbohydrates; D004396:Coloring Agents; D037102:Lectins; D013778:Tetrazolium Salts; D013844:Thiazoles; D003208:Concanavalin A; C022616:thiazolyl blue