Characterization of a human Sec14-like protein cDNA SEC14L3 highly homologous to human SPF/TAP.
Supernatant protein factor (SPF) and alpha-tocopherol-associated protein (TAP) both belong to a widespread lipid-binding Sec 14-like protein family. All the members of the family have the lipid-binding motif called CRAL_TRIO. SPF is showed to stimulate the conversion of squalene to lanosterol and enhance cholesterol biosynthesis. TAP is identified to be involved in the intracellular distribution of alpha-tocopherol. Recently TAP is identified as SPF though they have very different functions. Here we report a human SPF/TAP homology SEC14L3 with 2082 base pairs in length and contains an open reading frame encoding a 400 amino acids protein. Analysis shows that SEC14L3 is mapped to 22q12 and expresses only in the liver among the used sixteen tissues in the test.
Ye
Xin
X
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
Ji
Chaoneng
C
Yin
Gang
G
Tang
Rong
R
Zeng
Li
L
Gu
Shaohua
S
Ying
Kang
K
Xie
Yi
Y
Zhao
Robert Chunhua
RC
Mao
Yumin
Y
D018528:ATP-Binding Cassette Transporters; D000595:Amino Acid Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D002892:Chromosomes, Human, Pair 22; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006801:Humans; D050356:Lipid Metabolism; D008074:Lipoproteins / Q000378:metabolism*; D008099:Liver / Q000502:physiology; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; D002352:Carrier Proteins; D018076:DNA, Complementary; D015395:Histocompatibility Antigens Class I; D008074:Lipoproteins; D011506:Proteins; C432422:SEC14L2 protein, human; C487999:SEC14L3 protein, human; D015534:Trans-Activators; C477327:transporter associated with antigen processing (TAP)