title: Genetic relatedness among Campylobacter jejuni serotyped isolates of diverse origin as determined by numerical analysis of amplified fragment length polymorphism (AFLP) profiles.
abstract: OBJECTIVE
  To use amplified fragment length polymorphism (AFLP) analysis to evaluate the genetic relatedness among 254 Campylobacter jejuni reference and field strains of diverse origin representing all defined 'Penner' serotypes for this species.
  
  
  RESULTS
  Field strains (n = 207) from human diarrhoea and diverse animal and environmental sources were collected mainly through a National surveillance programme in Denmark and serotyped by use of the established 'Penner' scheme. Genetic relationships among these isolates, and the archetypal serotype reference strains, were assessed by numerical analysis of AFLP profiles derived from genomic DNA. Extensive genetic diversity was seen among the strains examined; however, 43 groups of isolates were identified at the 92% similarity (S-) level. Thirteen groups contained isolates from a single host, possibly representing genotypes of 'low risk' to human health. The remaining 30 groups contained isolates from humans, chickens and associated food products, cattle, sheep, turkeys, ostriches and/or dogs. Strains assigned to serotypes 2, 6/7, 11 and 12 formed major clusters at the 77.6% S-level. Most other serotypes did not form homogeneous clusters.
  
  
  CONCLUSIONS
  High-resolution genotyping applied to strains from a comprehensive range of sources provides evidence for multiple sources of sporadic C. jejuni infection. The results suggest that public health protection measures should be directed at all foods of animal origin.
  
  
  CONCLUSIONS
  The genetic relatedness among all 'Penner' serotypes of C. jejuni is assessed by AFLP analysis. In addition, further evidence of epidemic and host-specific clones of C. jejuni is provided.
authors.affiliation: Danish Veterinary Institute, Departments of Bacteriology, Copenhagen, Denmark.
authors.forename: B L
authors.initials: BL
authors.lastname: Siemer
authors.forename: C S
authors.initials: CS
authors.lastname: Harrington
authors.forename: E M
authors.initials: EM
authors.lastname: Nielsen
authors.forename: B
authors.initials: B
authors.lastname: Borck
authors.forename: N L
authors.initials: NL
authors.lastname: Nielsen
authors.forename: J
authors.initials: J
authors.lastname: Engberg
authors.forename: S L W
authors.initials: SL
authors.lastname: On
mesh_terms: D002169:Campylobacter Infections / Q000453:epidemiology / Q000382:microbiology*; D016123:Campylobacter jejuni / Q000235:genetics*; D003718:Denmark; D016680:Genome, Bacterial; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping