Extracellular matrix components in atherosclerotic arteries of Apo E/LDL receptor deficient mice: an immunohistochemical study.
During accelerated vascular remodeling such as in atherosclerosis, the composition of the extracellular matrix becomes altered. The matrix components of the diseased artery influence cellular processes such as adhesion, migration and proliferation. Furthermore, in atherosclerosis, the inability of the cells within the lesion to produce a mechanically stable matrix may lead to plaque rupture. In this immunohistochemical study of atherosclerotic mice aorta, we have reviewed the presence of ECM components with roles in maintaining tissue structure and function. These components include osteopontin and COMP as well as the leucine rich repeats proteins decorin, PRELP, and fibromodulin. Immunohistochemistry demonstrated presence of osteopontin, COMP, decorin, PRELP and fibromodulin in lesion areas of ApoE/LDLr deficient mice. Some advanced lesions exhibited areas of cartilage-like morphology and were shown to represent cartilage by their content of the cartilage specific proteins collagen II and aggrecan. The results suggest that cartilage-associated cell/collagen binding ECM proteins may be involved in the pathogenesis of atherosclerosis.
Ström
A
A
Department of Cell and Molecular Biology, Section for Connective Tissue Biology, Lund University, BMC, Lund, Sweden. asa.strom@medkem.lu.se
Ahlqvist
E
E
Franzén
A
A
Heinegård
D
D
Hultgårdh-Nilsson
A
A
D000818:Animals; D001011:Aorta / Q000378:metabolism* / Q000473:pathology; D001057:Apolipoproteins E / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D001161:Arteriosclerosis / Q000378:metabolism*; D015153:Blotting, Western; D002356:Cartilage / Q000378:metabolism; D002455:Cell Division; D003094:Collagen / Q000737:chemistry; D005109:Extracellular Matrix / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D000071235:Fibromodulin; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000378:metabolism; D007150:Immunohistochemistry; D064235:Matrilin Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D053495:Osteopontin; D011509:Proteoglycans / Q000378:metabolism; D011973:Receptors, LDL / Q000235:genetics / Q000502:physiology*; D012795:Sialoglycoproteins / Q000378:metabolism; D013997:Time Factors
D001057:Apolipoproteins E; D016326:Extracellular Matrix Proteins; C000604332:Fmod protein, mouse; D006023:Glycoproteins; C577540:Matn1 protein, mouse; D064235:Matrilin Proteins; D011509:Proteoglycans; D011973:Receptors, LDL; D012795:Sialoglycoproteins; C506896:Spp1 protein, mouse; D053495:Osteopontin; D000071235:Fibromodulin; D003094:Collagen