Observation on in situ hybridization and immunocytochemistry of matrix metalloproteinases in rat pancreas.
In situ hybridization and immunocytochemical techniques were employed to examine the expression of matrix metalloproteinases-1 (MMP-1) and to identify the pattern of its distribution in rat pancreas. The results indicated that the signal of MMP-1 mRNA and MMP-1 positive immunoreaction were detected in some fiberoblasts around interlobular ducts and exocrine cell in margin acinus of some lobules, but the signal of MMP-1 mRNA and MMP-1 positive immunoreaction could not be detected in most of other acinus and islets of pancreas. It is concluded that the expression of MMP-1 in above cells of rat might play an important role in acinar proliferation and differentiation of rat pancreatic tissues.
Tang
Lihua
L
Department of Histology and Embryology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
Liu
Shenghong
S
Wang
Fang
F
Liu
Zilong
Z
Xu
Yun
Y
Wang
Xiaoli
X
Li
Zhaochun
Z
D000818:Animals; D002455:Cell Division; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D010179:Pancreas / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D012333:RNA, Messenger; D020781:Matrix Metalloproteinase 1