Identification of an I(2) binding protein from rabbit brain.
Imidazoline-2 binding proteins exist as a heterogeneous population. The aim of this study was to isolate and identify I(2) binding proteins from rabbit brain using an affinity column synthesized with a highly selective I(2) ligand, 2-(2-benzofuranyl)2-imidazoline (2BFI). The results revealed an approximately 45-kD protein to be brain creatine kinase (EC 2.7.3.2). [(3)H]-2BFI (5nM) was able to bind specifically to the purified enzyme. This study has identified brain creatine kinase as a novel I(2) binding protein.
Kimura
A
A
Psychopharmacology Unit, School of Medical Sciences, University Walk, University of Bristol, Bristol BS8 1TD, UK.
Tyacke
R J
RJ
Minchin
M C W
MC
Nutt
D J
DJ
Hudson
A L
AL
D000818:Animals; D001572:Benzofurans / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D003402:Creatine Kinase / Q000737:chemistry / Q000378:metabolism; D007093:Imidazoles / Q000378:metabolism; D054755:Imidazoline Receptors; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011485:Protein Binding; D020033:Protein Isoforms / Q000378:metabolism; D011817:Rabbits; D011955:Receptors, Drug / Q000378:metabolism*
D001572:Benzofurans; D007093:Imidazoles; D054755:Imidazoline Receptors; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D011955:Receptors, Drug; C103723:2-(2-benzofuranyl)-2-imidazoline; D003402:Creatine Kinase