[Usefulness of immunoplatelet measurement using the flow cytometric method for accurate platelet counting in hematological patients with severe thrombocytopenia].
We measured platelet counts in 95 patients with hematological disorders accompanied by thrombocytopenia (platelet counts < 5.0 x 10(4)/microliter) including 35 patients with severe thrombocytopenia(platelet counts < 2.0 x 10(4)/microliter). We used four methods based on different principles and compared the results, i.e., the flow cytometric method (BEADS method) utilizing platelet-specific monoclonal antibody (SZ2, antiGPIb) in conjunction with fluorescent reference beads (Flow-Count Fluorospheres), manual hemocytometry, and two automated blood cell counters, the NE-8000 (impedance method) and the Technicon H-2 (optical method). The BEADS method was superior to the other methods in linearity of serial dilutions, and the coefficient variations of the BEADS method(2.5-5.2%) were superior to the other methods. The platelet counts measured by the automated blood cell counters were higher(0.6-0.9 x 10(4)/microliter) than those by the BEADS method and manual hemocytometry. Furthermore, the BEADS method was able to measure accurate platelet counts in samples containing red blood cell fragments. The BEADS method may be an accurate and useful method for measuring samples with severe thrombocytopenia, and, especially, samples containing red blood cell fragments.
Nishiyama
Miho
M
Central Laboratory for Clinical Investigation, Osaka University Hospital, Suita 565-0871.
Hayashi
Satoru
S
Futsukaichi
Yoshiaki
Y
Suehisa
Etsuji
E
Kurata
Yoshiyuki
Y
D000328:Adult; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D010976:Platelet Count / Q000295:instrumentation / Q000379:methods*; D012720:Severity of Illness Index; D013921:Thrombocytopenia / Q000097:blood*