title: Human herpesvirus 6 immediate-early 2 protein interacts with heterogeneous ribonucleoprotein K and casein kinase 2.
abstract: Human herpesvirus 6 (HHV-6) immediate-early (IE) 2 protein (IE2) may play important but incompletely defined roles during infection. We used yeast two-hybrid screening to detect proteins interacting with HHV-6 IE2, and found heterogeneous nuclear ribonucleoprotein K (hnRNP K) and the beta subunit of casein kinase 2 (CK2beta) specifically interacted with HHV-6 IE2. The interactions were confirmed by GST pull-down assay, coimmunoprecipitation, and colocalization studies. These findings indicate that the HHV-6 IE2 protein interacts with hnRNP K and CK2, and these interactions may affect viral and cellular RNA transcription and translation in viral replication.
authors.affiliation: Department of Microbiology, Osaka University Medical School C1, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Shimada
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kondo
authors.forename: Koichi
authors.initials: K
authors.lastname: Yamanishi
mesh_terms: D047390:Casein Kinase II; D015654:Herpesvirus 6, Human / Q000737:chemistry* / Q000235:genetics; D034641:Heterogeneous-Nuclear Ribonucleoprotein K; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism*
chemical_list: D034641:Heterogeneous-Nuclear Ribonucleoprotein K; D017874:Immediate-Early Proteins; D010446:Peptide Fragments; D012261:Ribonucleoproteins; C105173:casein kinase 2 beta (1-16); C072074:HNRNPK protein, human; D011494:Protein Kinases; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases