CD30 is involved in inhibition of T-cell proliferation by Hodgkin's Reed-Sternberg cells.
CD30 is expressed on Hodgkin's Reed-Sternberg (H-RS) cells, the tumor cells in Hodgkin's disease. Increased levels of serum CD30 are observed in Hodgkin's disease patients and are a good marker for predicting a poor prognosis and a poor response to therapy. In this study, we addressed the effect of CD30 on T cells. We showed that CD30, either as a membranous protein on H-RS cells and Chinese hamster ovary cells or as a plate-bound chimeric protein, inhibited T-cell proliferation. Anti-CD3-stimulated T cells in the presence of CD30 failed to increase tritium uptake and failed to express CD25 and CD26 and to produce interleukin 2. The inhibition of T-cell proliferation was, however, reversed with addition of exogenous interleukin 2 or pretreatment of H-RS cells with anti-CD30. Inability of T cells to express CD25 and CD26 in cocultures with H-RS cells or a plate-bound CD30 chimeric protein is in accordance with the results of immunohistochemistry on disease-involved tissues. We conclude that H-RS cells are able to inhibit the proliferation and activation of T cells through CD30-related interaction. The outcome of CD30-related interaction is an ineffective antitumor immunity, which is clearly in favor of the growth and survival of the tumor cells.
Su
Che-Chun
CC
Graduate Institute of Immunology, National Taiwan University College of Medicine, 1 Jen-Ai Road, Taipei, Taiwan.
Chiu
Hsiu-Hui
HH
Chang
Chia-Che
CC
Chen
Jui-Chieh
JC
Hsu
Su-Ming
SM
D000818:Animals; D016466:CHO Cells; D002455:Cell Division; D018920:Coculture Techniques; D006224:Cricetinae; D006689:Hodgkin Disease / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism / Q000494:pharmacology; D017730:Ki-1 Antigen / Q000502:physiology*; D008213:Lymphocyte Activation / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D016539:Reed-Sternberg Cells / Q000276:immunology* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology*
D007376:Interleukin-2; D017730:Ki-1 Antigen; D011993:Recombinant Fusion Proteins