Development of a polar morphology by identified embryonic motoneurons.
Motoneuron morphology arises through the coordinated growth of the motor axon and dendrites. In the Drosophila embryo the RP motoneurons have a contralaterally-extended motor axon, ipsilateral dendrites that extend a short distance in the ipsilateral connective, and a tuft of short dendrites in the contralateral connective. In the present study mechanical and genetic manipulations were utilized to test if (i) the ipsilateral dendrites can develop an axon morphology, (ii) the presence of the contralateral motor axon suppresses the development of an axon-like morphology by the ipsilateral dendrites and (iii) whether establishment of a contralateral motor axon can be genetically suppressed. It was found that an ipsilateral motor axon could develop-but only at the expense of the contralateral motor axon. Axotomy could overturn the normal polarity of the RP motoneurons in favor of the development of an ipsilateral motor axon, and this reversed morphology was also observed when the motor axon could not extend across the midline in the commissureless mutant. These findings show that the RP motoneurons have the plasticity for an alternative polarity, but that the extension of an ipsilateral axon is normally suppressed by the presence of the contralateral axon. The RP motoneurons now represent a genetically amenable in vivo system for analyzing the basis of polarity formation in neurons.
Whitington
Paul M
PM
Department of Anatomy and Cell Biology, University of Melbourne, Melbourne, Victoria 3010 Australia.
Sink
Helen
H
D000818:Animals; D030801:Animals, Genetically Modified; D001369:Axons / Q000502:physiology; D019771:Axotomy / Q000379:methods; D003712:Dendrites / Q000378:metabolism; D004330:Drosophila; D029721:Drosophila Proteins; D004625:Embryo, Nonmammalian / Q000502:physiology*; D007839:Functional Laterality / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods; D007546:Isoquinolines / Q000493:pharmacokinetics; D008565:Membrane Proteins / Q000235:genetics; D008959:Models, Neurological; D009046:Motor Neurons / Q000502:physiology*
D029721:Drosophila Proteins; D007546:Isoquinolines; D008565:Membrane Proteins; C110665:comm protein, Drosophila; C017475:lucifer yellow