title: Esterification of vertebrate-type steroids in the Eastern oyster (Crassostrea virginica).
abstract: Characteristics of acyl-coenzyme A (acyl-CoA):steroid acyltransferase from the digestive gland of the oyster Crassostrea virginica were determined by using estradiol (E2) and dehydroepiandrosterone (DHEA) as substrates. The apparent Km and Vmax values for esterification of E2 with the six fatty acid acyl-CoAs tested (C20:4, C18:2, C18:1, C16:1, C18:0, and C16:0) were in the range of 9-17 microM E2 and 35-74 pmol/min/mg protein, respectively. Kinetic parameters for esterification of DHEA (Km: 45-120 microM; Vmax: 30-182 pmol/min/mg protein) showed a lower affinity of the enzyme for this steroid. Formation of endogenous fatty acid esters of steroids by microsomes of digestive gland and gonads incubated in the presence of ATP and CoA was assessed, and at least seven E2 fatty acid esters and five DHEA fatty acid esters were observed. Some peaks eluted at the same retention times as palmitoleoyl-, linoleoyl-, oleoyl/palmitoyl-, and stearoyl-E2; and palmitoleoyl-, oleoyl/palmitoyl-, and stearoyl-DHEA. The same endogenous esters, although in different proportions, were produced by gonadal microsomes. The kinetic parameters for both E2 (Km: 10 microM; Vmax: 38 pmol/min/mg protein) and DHEA (Km: 61 microM; Vmax: 60 pmol/min/mg protein) were similar to those obtained in the digestive gland. Kinetic parameters obtained are similar to those observed in mammals; thus, fatty acid esterification of sex steroids appears to be a well-conserved conjugation pathway during evolution.
authors.affiliation: Department of Environmental Chemistry, IIQAB-CSIC, Jordi Girona 18, 08034 Barcelona, Spain. gjgqam@cid.csic.es
authors.forename: Gemma
authors.initials: G
authors.lastname: Janer
authors.forename: Sonia
authors.initials: S
authors.lastname: Mesia-Vela
authors.forename: Cinta
authors.initials: C
authors.lastname: Porte
authors.forename: Frederick C
authors.initials: FC
authors.lastname: Kauffman
mesh_terms: D000217:Acyltransferases / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003687:Dehydroepiandrosterone / Q000378:metabolism; D004951:Esterification; D004958:Estradiol / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D007700:Kinetics; D008861:Microsomes; D010125:Ostreidae / Q000737:chemistry / Q000378:metabolism*; D013256:Steroids / Q000378:metabolism*; D014714:Vertebrates
chemical_list: D005227:Fatty Acids; D013256:Steroids; D003687:Dehydroepiandrosterone; D004958:Estradiol; D000217:Acyltransferases