Determination of nucleic acids with tetra-(N-hexadecylpyridiniumyl) porphyrin sensitized by cetyltrimethylammonium bromide (CTMAB) using a Rayleigh light-scattering technique.
Using a common spectrofluorometer to measure the intensity of Rayleigh light-scattering (RLS), a method for determination of nucleic acids has been developed. At pH 10.24 and ionic strength 0.01 mol l-1 (NaCl), the Rayleigh light-scattering of the tetra-(N-hexadecylpyridiniumyl) porphyrin (TC16PyP) is greatly enhanced by nucleic acids in the presence of cetyltrimethylammonium bromide (CTMAB), with the scattering peak located at 311.8 nm. The enhanced RLS intensity is in proportion to the concentration of calf thymus DNA (ctDNA) in the range 0.2-6.0 microg ml-1 and to that of fish sperm DNA (fsDNA) in the range 0.05-3.0microg ml-1. The limits of detection are 0.016 microg ml-1 for calf thymus DNA and 0.023 microg ml-1 for fish sperm DNA when the concentration of TPP was chosen 2.0 x 10(-6) mol l-1. Four synthetic samples were determined satisfactorily.
Zhu
Changqing
C
College of Chemistry and Materials Science, Anhui Normal Unversity, Wuhu 241000, PR China. zhucq625@163.com
Zhuo
Shujuan
S
Li
Yongxin
Y
Wang
Lun
L
Zhao
Danhua
D
Chen
Jinlong
J
Wu
Yuqin
Y
D000818:Animals; D002138:Calibration; D002417:Cattle; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry* / Q000494:pharmacology; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D003902:Detergents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005399:Fishes; D006863:Hydrogen-Ion Concentration; D007477:Ions; D008027:Light; D008297:Male; D008956:Models, Chemical; D009696:Nucleic Acids / Q000032:analysis* / Q000737:chemistry; D011166:Porphyrins / Q000032:analysis / Q000737:chemistry*; D011726:Pyridinium Compounds / Q000032:analysis / Q000737:chemistry*; D012542:Scattering, Radiation; D012965:Sodium Chloride / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*; D013094:Spermatozoa / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism
D002593:Cetrimonium Compounds; D003902:Detergents; D007477:Ions; D009696:Nucleic Acids; D011166:Porphyrins; D011726:Pyridinium Compounds; C492642:tetra-(N-hexadecylpyridiniumyl) porphyrin; D012965:Sodium Chloride; D004247:DNA; D000077286:Cetrimonium