title: Implication of ZW10 in membrane trafficking between the endoplasmic reticulum and Golgi.
abstract: ZW10, a dynamitin-interacting protein associated with kinetochores, is known to participate directly in turning off of the spindle checkpoint. In the present study, we show that ZW10 is located in the endoplasmic reticulum as well as in the cytosol during interphase, and forms a subcomplex with RINT-1 (Rad50-interacting protein) and p31 in a large complex comprising syntaxin 18, an endoplasmic reticulum-localized t-SNARE implicated in membrane trafficking. Like conventional syntaxin-binding proteins, ZW10, RINT-1 and p31 dissociated from syntaxin 18 upon Mg(2+)-ATP treatment in the presence of NSF and alpha-SNAP, whereas the subcomplex was not disassembled. Overexpression, microinjection and knockdown experiments revealed that ZW10 is involved in membrane trafficking between the endoplasmic reticulum and Golgi. The present results disclose an unexpected role for a spindle checkpoint protein, ZW10, during interphase.
authors.affiliation: School of Life Science, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Hirose
authors.forename: Kohei
authors.initials: K
authors.lastname: Arasaki
authors.forename: Naoshi
authors.initials: N
authors.lastname: Dohmae
authors.forename: Koji
authors.initials: K
authors.lastname: Takio
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Hatsuzawa
authors.forename: Masami
authors.initials: M
authors.lastname: Nagahama
authors.forename: Katsuko
authors.initials: K
authors.lastname: Tani
authors.forename: Akitsugu
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Masaya
authors.initials: M
authors.lastname: Tohyama
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Tagaya
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism; D002460:Cell Line; D000072159:Dynactin Complex; D004398:Dyneins / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006056:Golgi Apparatus / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007399:Interphase; D007425:Intracellular Membranes / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009687:Nuclear Proteins; D011485:Protein Binding; D021381:Protein Transport; D050765:Qa-SNARE Proteins; D020798:Two-Hybrid System Techniques
chemical_list: D002352:Carrier Proteins; D018797:Cell Cycle Proteins; C000606361:DCTN2 protein, human; D000072159:Dynactin Complex; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D050765:Qa-SNARE Proteins; C422909:RINT1 protein, human; C413695:ZWINT protein, human; D004398:Dyneins