Detection of Fasciola gigantica infection in snails by polymerase chain reaction.
Polymerase chain reaction (PCR) was used to detect Fasciola gigantica infection in the snail intermediate host. Fasciola specific primers amplified a 124 bp fragment in PCR when the genomic DNA isolated from F. gigantica infected Lymnaea auricularia snails was used as template. In addition to the 124 bp amplicon, a ladder of DNA fragments representing amplification of the 124 bp repetitive sequences was observed. Genomic DNA of the parasite was used as a positive control, which also gave an amplification of the 124 bp fragment. DNA isolated from non-infected snails was used as a negative control and no amplification of this sequence was observed. This technique is highly specific and sensitive and possesses fairly good prospects of its utility as an epidemiological tool for ascertaining the infectivity status in ubiquitous snail populations.
Velusamy
R
R
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, Bareilly, UP 243122, India.
Singh
B P
BP
Raina
O K
OK
D000818:Animals; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005209:Fasciola / Q000235:genetics / Q000302:isolation & purification*; D005211:Fascioliasis / Q000469:parasitology / Q000662:veterinary*; D008195:Lymnaea / Q000469:parasitology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics
D018509:DNA, Helminth