Oral glucose tolerance test and determination of serum fructosamine level in beagle dogs.
The present communication deals with information regarding the practice of the oral glucose tolerance test and determination of serum fructosamine in laboratory beagles. In the oral glucose tolerance test, a 180-min level was found to be crucial following a gavage administration of 50% glucose solution at 5 mL/kg per body weight under fasting conditions. Serum fructosamine concentration as 'determined by enzymatic assay ranged between 82 and 123 micromol/L (mean of 104 micromol/L), which was about 0.285 to 0.25 times the value obtained by the chemical method described by Johnson and colleagues. Reasons for differences are ascribed to the presence of substances with reducing potential other than fructosamine in the serum.
Watanabe
Dai
D
Biology and Zoology Research Center Inc., Kannami Laboratory, 1308 Sanbonmatsu, Kuwabara, Kannami-cho, Tagata-gun, Shizuoka 419-0101, Japan.
Nakara
Hiromi
H
Akagi
Keisuke
K
Ishii
Toshiya
T
Mizuguchi
Hiroyasu
H
Nagashima
Yoshikazu
Y
Okaniwa
Azusa
A
D000284:Administration, Oral; D000818:Animals; D004285:Dogs; D005215:Fasting; D005260:Female; D005508:Food Deprivation; D019270:Fructosamine / Q000097:blood*; D005947:Glucose / Q000008:administration & dosage; D005951:Glucose Tolerance Test / Q000379:methods* / Q000662:veterinary*; D008297:Male
D019270:Fructosamine; D005947:Glucose