DNA microarray analysis of the correlation between gene expression patterns and acquired resistance to 5-FU/cisplatin in gastric cancer.
The mechanisms of intrinsic and/or acquired anti-cancer drug resistance have been described in in vitro resistance models, but the clinical relevance has remained undefined. We undertook a prospective study to identify correlations between gene expression and clinical resistance to 5-FU/cisplatin. We compared expression profiles from gastric cancer endoscopic biopsy specimens obtained at a chemosensitive state (partial remission after 5-FU/cisplatin) with those obtained at a refractory state (disease progression), using Affymetrix oligonucleotide microarray technology (U133A). Using 119 discriminating probes and a cross-validation approach, we were able to correctly identify the chemo-responsiveness of 7 pairs of training samples and 1 independent test pair. These exploratory data demonstrate that the gene expression profiles differ between chemosensitive and refractory state gastric cancer biopsy samples.
Kim
Hark Kyun
HK
Research Institute and Hospital, National Cancer Center, Goyang, Gyeonggi, Republic of Korea.
Choi
Il Ju
IJ
Kim
Hee Sung
HS
Kim
Ju Han
JH
Kim
Eugene
E
Park
In Sook
IS
Chun
Jong Ho
JH
Kim
In-Hoo
IH
Kim
Il-Jin
IJ
Kang
Hio Chung
HC
Park
Jae-Hyun
JH
Bae
Jae-Moon
JM
Lee
Jin Soo
JS
Park
Jae-Gahb
JG
D000328:Adult; D000368:Aged; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D001706:Biopsy; D016000:Cluster Analysis; D018450:Disease Progression; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm; D004724:Endoscopy; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics*; D015854:Up-Regulation
D000964:Antimetabolites, Antineoplastic; D005472:Fluorouracil