title: Nitrogen catabolite repression in Saccharomyces cerevisiae during wine fermentations.
abstract: We carried out fermentations with several nitrogen sources in different concentrations and studied nitrogen regulation by following the transcriptional profile of the general amino-acid permease (GAP1) and the ammonium permeases (MEP1, MEP2, MEP3). In wine fermentations the cells evolve from a nitrogen-repressed situation at the beginning of the process to a nitrogen-derepressed situation as the nitrogen is consumed. These nitrogen-repressed/derepressed conditions determined the different patterns of ammonium and amino-acid consumption. Arginine and alanine were hardly used under the repressed conditions, while the uptake of branched-chain and aromatic amino acids increased.
authors.affiliation: Unitat d'Enologia del Centre de Referència de Tecnologia d'Aliments, Dept. Bioquímica i Biotecnologia, Facultat d'Enologia de Tarragona, Universitat Rovira i Virgili, Ramón y Cajal, 70, 43005 Tarragona, Spain.
authors.forename: Gemma
authors.initials: G
authors.lastname: Beltran
authors.forename: Maite
authors.initials: M
authors.lastname: Novo
authors.forename: Nicolas
authors.initials: N
authors.lastname: Rozès
authors.forename: Albert
authors.initials: A
authors.lastname: Mas
authors.forename: José M
authors.initials: JM
authors.lastname: Guillamón
mesh_terms: D026905:Amino Acid Transport Systems / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D027682:Cation Transport Proteins / Q000378:metabolism; D005285:Fermentation; D015966:Gene Expression Regulation, Fungal; D009584:Nitrogen / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D014920:Wine / Q000382:microbiology*
chemical_list: D026905:Amino Acid Transport Systems; D000596:Amino Acids; D027682:Cation Transport Proteins; C088951:MEP1 protein, S cerevisiae; D000644:Quaternary Ammonium Compounds; D029701:Saccharomyces cerevisiae Proteins; D009584:Nitrogen