title: Protein-trap version 2.1: screening for expressed proteins in mammalian cells based on their localizations.
abstract: BACKGROUND
  "Protein-trap" is a method that allows epitope-tagging of endogenous proteins. This method allows for the identification of endogenously expressed proteins that exhibit specific localization of interest. This method has been recently reported for its application in the study of Drosophila development by using a relatively large epitope, green-fluorescent-protein (GFP).
  
  
  RESULTS
  Herein, we report a new "protein-trap" vector for mammalian cells. This new method utilizes a much smaller epitope-tag and also allows for drug-selection prior to the epitope-tagging. Pre-selection by drug resulted in the highly efficient protein-trapping frequency.
  
  
  CONCLUSIONS
  The "protein-trap" method based on this new vector is expected to serve as a complimentary approach to the previously reported GFP-based method.
authors.affiliation: The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA. Olga.Sineshchekova@UTSouthwestern.edu
authors.forename: Olga O
authors.initials: OO
authors.lastname: Sineshchekova
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Kawate
authors.forename: Oleksandr V
authors.initials: OV
authors.lastname: Vdovychenko
authors.forename: Thomas N
authors.initials: TN
authors.lastname: Sato
mesh_terms: D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002277:Carcinoma / Q000737:chemistry / Q000235:genetics / Q000473:pathology / Q000821:virology; D003001:Cloning, Molecular / Q000379:methods*; D000939:Epitopes / Q000096:biosynthesis / Q000235:genetics; D005091:Exons / Q000235:genetics / Q000276:immunology; D005109:Extracellular Matrix / Q000502:physiology; D005822:Genetic Vectors / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D042541:Intracellular Space / Q000737:chemistry; D008175:Lung Neoplasms / Q000737:chemistry / Q000188:drug therapy / Q000235:genetics / Q000473:pathology; D014176:Protein Biosynthesis; D021381:Protein Transport / Q000502:physiology; D011506:Proteins / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D012190:Retroviridae / Q000235:genetics; D015398:Signal Transduction / Q000502:physiology; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D011506:Proteins; D011993:Recombinant Fusion Proteins