Formation and analysis of heterocyclic aromatic amine-DNA adducts in vitro and in vivo.
The detection and quantification of heterocyclic aromatic amine (HAA)-DNA adducts, critical biomarkers in interspecies extrapolation of toxicity data for human risk assessment, remains a challenging analytical problem. The two main analytical methods currently in use to screen for HAA-DNA adducts are the 32P-postlabeling assay and mass spectrometry, using either accelerated mass spectrometry (AMS) or liquid chromatography and electrospray ionization mass spectrometry (LC-ESI-MS). In this review, the principal methods to synthesize and characterize DNA adducts, and the methods applied to measure HAA-DNA adduct in vitro and vivo are discussed.
Turesky
Robert J
RJ
Division of Chemistry, National Center for Toxicological Research, 3900 NCTR Road, Jefferson, AR 72079, USA. rturesky@nctr.fda.gov
Vouros
Paul
P
D000588:Amines / Q000737:chemistry*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D018736:DNA Adducts / Q000032:analysis / Q000737:chemistry*; D006801:Humans; D021241:Spectrometry, Mass, Electrospray Ionization
D000588:Amines; D018736:DNA Adducts