title: [Detection of IgH gene rearrangement in Reed-Sternberg cells microdissected from classical Hodgkin's lymphoma].
abstract: OBJECTIVE
  To study the origins and clonality of Hodgkin and Reed-Sternberg (H/RS) cells and their relations with the background lymphocytes in classical Hodgkin's lymphoma.
  
  
  METHODS
  IgH gene rearrangement was detected in paffin-embedded tissues from 33 patients with Hodgkin's lymphoma and a further analysis of the gene rearrangement was conducted in 6 of the positive cases identified after immunostaining of the sections with B-cell-specific activator protein (BSAP) followed by microdissection of the positivity labeled H/RS cells and background lymphocytes.
  
  
  RESULTS
  IgH gene rearrangement was identified in 16 of the 33 cases. Microdissection of the lymphoma tissues was successfully performed in the 6 positive cases, and of the 19 tubes of H/RS cells obtained, 14 presented clonal bands of the rearrangement, and difference in cell numbers did not significantly influence the positive rate (P=0.290); in the 12 tubes of microdissected background lymphocytes obtained, 2 were positive for the rearrangement, and the positive rates for the rearrangement significantly differed between H/RS cells and the background lymphocytes (P=0.002).
  
  
  CONCLUSIONS
  The results appear to support the hypothesis that H/RS cells originates from B cells, and a part of the background lymphocytes may possess neoplastic proliferation potentials to function as the precursors of H/RS cells.
authors.affiliation: Department of Pathology, First Military Medical University, Guangzhou 510515, China. balbc@fimmu.com
authors.forename: Xinhua
authors.initials: X
authors.lastname: Zhou
authors.forename: Tong
authors.initials: T
authors.lastname: Zhao
authors.forename: Xin-ming
authors.initials: XM
authors.lastname: Shen
authors.forename: Jiang
authors.initials: J
authors.lastname: Yu
authors.forename: Mei-gang
authors.initials: MG
authors.lastname: Zhu
mesh_terms: D000293:Adolescent; D000328:Adult; D001402:B-Lymphocytes / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005260:Female; D015321:Gene Rearrangement; D006689:Hodgkin Disease / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D008297:Male; D042282:Microdissection; D008875:Middle Aged; D016539:Reed-Sternberg Cells / Q000378:metabolism*
chemical_list: D007143:Immunoglobulin Heavy Chains