[Effect of buyang huanwu decoction drug serum on expression of p53 and p21 genes in cultured rat's cerebral cortical neuron after hypoxia in vitro].
OBJECTIVE
To explore the effect of Buyang Huanwu decoction (BHD) drug serum on rat's in vitro cultured cerebral cortical neuron apoptosis induced by hypoxia, and on the expression of p53 and p21 genes in hypoxia process.


METHODS
The model of hypoxia neuron apoptosis was established adopting Daniel method and treated with BHD drug serum. The neuron apoptosis rate was determined by flow cytometry with propidium iodide staining, the p53 and p21 gene expression was tested by immunohistochemical method with flow cytometry.


RESULTS
BHD could significantly inhibit the neuron apoptosis induced by hypoxia and down-regulate the expressions of p53 and p21 genes.


CONCLUSIONS
BHD shows inhibition on neuron hypoxia apoptosis and down-regulating of the p53 and p21 gene expression is one of its mechanisms.
Qu
Hong-da
HD
Faculty of TCM, First Military Medical University, Guangzhou 510515. qhd2@263.net
Tong
Li
L
Shen
Jian-gang
JG
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015687:Cell Hypoxia; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000473:pathology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D008297:Male; D009474:Neurons / Q000473:pathology; D015689:Oncogene Protein p21(ras) / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D044967:Serum; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000235:genetics
D004365:Drugs, Chinese Herbal; D016159:Tumor Suppressor Protein p53; C460102:buyang huanwu; D015689:Oncogene Protein p21(ras)