title: Protective effects of Brussels sprouts, oligosaccharides and fermented milk towards 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced genotoxicity in the human flora associated F344 rat: role of xenobiotic metabolising enzymes and intestinal microflora.
abstract: We investigated the chemoprotective effects of four common constituents of the human diet, i.e. a fermented milk, inulin, oligofructose and Brussels sprouts, towards 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced genotoxicity in male Fischer 344 rats harbouring a human intestinal microflora. We found that the four dietary components significantly reduced IQ-induced DNA damage in hepatocytes (reduction ranged from 74% with inulin to 39% with Brussels sprouts) and colonocytes (reduction ranged from 68% with inulin to 56% with Brussels sprouts). This chemoprotective effect correlated with the induction of hepatic UDP-glucuronosyl transferase following Brussels sprouts consumption, and with alterations of bacterial metabolism in the distal gut (acidification, increase of butyrate proportion, decrease of beta-glucuronidase activity) following inulin consumption.
authors.affiliation: Institut National de la Recherche Agronomique, Unité d'Ecologie et de Physiologie du Système Digestif, Bâtiment Jacques Poly, 78352 Jouy-en-Josas, France.
authors.forename: Christèle
authors.initials: C
authors.lastname: Humblot
authors.forename: Evelyne
authors.initials: E
authors.lastname: Lhoste
authors.forename: Siegfried
authors.initials: S
authors.lastname: Knasmüller
authors.forename: Karine
authors.initials: K
authors.lastname: Gloux
authors.forename: Aurélia
authors.initials: A
authors.lastname: Bruneau
authors.forename: Martine
authors.initials: M
authors.lastname: Bensaada
authors.forename: José
authors.initials: J
authors.lastname: Durao
authors.forename: Sylvie
authors.initials: S
authors.lastname: Rabot
authors.forename: Claude
authors.initials: C
authors.lastname: Andrieux
authors.forename: Fekadu
authors.initials: F
authors.lastname: Kassie
mesh_terms: D000818:Animals; D001937:Brassica; D004249:DNA Damage; D004586:Electrophoresis / Q000379:methods; D005285:Fermentation; D005966:Glucuronidase / Q000378:metabolism; D008892:Milk; D009844:Oligosaccharides / Q000494:pharmacology*; D011804:Quinolines / Q000633:toxicity*; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D009844:Oligosaccharides; D011804:Quinolines; C029216:2-amino-3-methylimidazo(4,5-f)quinoline; D005966:Glucuronidase