title: Molecular cloning of low-temperature-inducible ribosomal proteins from soybean.
abstract: Three ribosomal protein genes induced by low-temperature treatment were isolated from soybean. GmRPS13 (742 bp) encodes a 17.1 kDa protein which has 95% identity with the 40S ribosomal protein S13 of Panax ginseng (AB043974). GmRPS6 (925 bp) encodes a 28.1 kDa protein which has 94% identity with the 40S ribosomal protein S6 of Asparagus officinalis (AJ277533). GmRPL37 (494 bp) encodes a 10.7 kDa protein which has 85% identity with the 60S ribosomal protein L37 of Arabidopsis thaliana (AF370216). The expression of these ribosomal protein genes started to increase 3 d after low-temperature treatment, whereas the cold-stress protein src1 was highly induced from the first day. Such late response of these ribosomal protein genes may be due to secondary signals during cold adaptation. The induction of ribosomal protein genes might enhance the translation process or help proper ribosome functioning under low-temperature conditions.
authors.affiliation: Department of Plant Biotechnology, Dong-A University, Busan, 604-714, South Korea.
authors.forename: Kee-Young
authors.initials: KY
authors.lastname: Kim
authors.forename: Seong-Whan
authors.initials: SW
authors.lastname: Park
authors.forename: Young-Soo
authors.initials: YS
authors.lastname: Chung
authors.forename: Chung-Han
authors.initials: CH
authors.lastname: Chung
authors.forename: Jung-In
authors.initials: JI
authors.lastname: Kim
authors.forename: Jai-Heon
authors.initials: JH
authors.lastname: Lee
mesh_terms: D003001:Cloning, Molecular / Q000379:methods; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics*; D013025:Glycine max / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D012269:Ribosomal Proteins