title: Glycoxidation of low-density lipoprotein increases TUNEL positivity and CPP32 activation in human coronary cells.
abstract: Apoptosis of arterial cells induced by oxidized low-density lipoprotein (oxLDL) is thought to contribute to the progression of vascular dysfunction and atherogenesis. It is well established that diabetes mellitus is accompanied by both glycosylation and oxidation of LDL (glc-oxLDL), but the biological effects of these modified lipoproteins are poorly understood. We demonstrate here for the first time that glc-oxLDL increases TUNEL positivity and caspase-3 activation (by Western blot and immunocytochemistry) of human coronary smooth muscle cells. Overall, these effects induced by glc-oxLDL were greater than those achieved with oxLDL. Thus, glc-oxLDL activated downstream apoptotic signaling. This may influence the evolution of atherogenesis and vascular complications in diabetes.
authors.affiliation: Department of Pharmacological Sciences, Chair of Anatomy, Faculty of Pharmacy, University of Salerno, Fisciano-Salerno, Italy. fnigris@yahoo.com
authors.forename: Filomena
authors.initials: F
authors.lastname: de Nigris
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Tajana
authors.forename: Mario
authors.initials: M
authors.lastname: Condorelli
authors.forename: Francesco P
authors.initials: FP
authors.lastname: D'Armiento
authors.forename: Giacomo
authors.initials: G
authors.lastname: Sica
authors.forename: Lilach O
authors.initials: LO
authors.lastname: Lerman
authors.forename: Claudio
authors.initials: C
authors.lastname: Napoli
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism*; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000166:cytology* / Q000201:enzymology / Q000502:physiology*; D004789:Enzyme Activation; D005434:Flow Cytometry; D006031:Glycosylation; D006801:Humans; D020287:In Situ Nick-End Labeling; D008077:Lipoproteins, LDL / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000201:enzymology / Q000502:physiology; D010084:Oxidation-Reduction
chemical_list: D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases