Sequential oligopeptide carriers, SOCn, as scaffolds for the reconstitution of antigenic proteins: applications in solid phase immunoassays.
A new class of helicoid type sequential oligopeptide carriers (SOC), for anchoring antigenic epitopes, has been modeled from the repetitive Lys-Aib-Gly (SOC(n)-I) and Aib-Lys-Aib-Gly (SOC(n)-II) units aiming to the development of scaffolds with predetermined 3D structures. Conformational analysis showed that the SOC(n) carriers adopt 3(10)-helical structures, while the SOC(n)-conjugates retain their original active conformations and they interact neither to the carriers nor to each other. It is concluded that the helicoid structure of SOC(n) helps the reconstitution and/or mimicking of the native forms of the epitopes so that potent antigens are generated for developing specific, sensitive and reproducible immunoassays.
Sakarellos-Daitsiotis
Maria
M
Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, 45 110 Ioannina, Greece. msakarel@cc.uoi.gr
Alexopoulos
Charalampos
C
Sakarellos
Constantinos
C
D000595:Amino Acid Sequence; D000941:Antigens / Q000737:chemistry* / Q000378:metabolism; D004337:Drug Carriers / Q000138:chemical synthesis / Q000145:classification / Q000378:metabolism*; D000939:Epitopes / Q000502:physiology; D006115:Greece; D007118:Immunoassay / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism
D000941:Antigens; D004337:Drug Carriers; D000939:Epitopes; D009842:Oligopeptides; D010455:Peptides