Simultaneous determination of ginsenoside Rb(1) and Rg(1) in human plasma by liquid chromatography-mass spectrometry.
A liquid chromatographic-mass spectrometric (LC/MS) method for the simultaneous determination of ginsenoside Rb(1) and Rg(1) in human plasma was developed. The method involved the protein precipitation followed by analysis of ginsenoside Rb(1) and Rg(1) in an Atlantis C(18) column with the gradient elution of acetonitrile and ammonium formate (10mM, pH 3.0) at a flow rate of 0.2 ml/min. The analytes were determined using electrospray negative ionization mass spectrometry in the selected ion monitoring mode. The standard curves for ginsenoside Rb(1) and Rg(1) were linear over the concentration range of 10.0-1000 ng/ml. The lower limit of quantification was 10.0 ng/ml using 100 microl plasma sample. The coefficient of variation of intra- and inter-day assays for ginsenoside Rb(1) and Rg(1) at three quality control levels ranged from 1.0 to 6.8% and 5.4 to 9.8%, respectively. Ginsenoside Rb(1) and Rg(1) were stable in blank human plasma at room temperature for 24h and following three freeze-thaw cycles.
Ji
Hye Young
HY
College of Pharmacy, Wonkwang University, Shinyongdong 344-2, Jeonbuk, Iksan 570749, South Korea.
Lee
Hye Won
HW
Kim
Hae Kyoung
HK
Kim
Hui Hyun
HH
Chang
Seung Goo
SG
Sohn
Dong Hwan
DH
Kim
Jaebaek
J
Lee
Hye Suk
HS
D002138:Calibration; D002853:Chromatography, Liquid; D036145:Ginsenosides / Q000097:blood*; D006801:Humans; D008297:Male; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D021241:Spectrometry, Mass, Electrospray Ionization
D036145:Ginsenosides; C048026:ginsenoside Rb2; C442759:ginsenoside Rb1