A sandwich ELISA for the estimation of human syndecan-2 and syndecan-4 in biological samples.
Syndecans are integral membrane proteoglycans containing both chondroitin and heparan sulphate side chains. Syndecans are shed from the cell surface, and may be detected in cellular supernatant or body fluids. In this study, we wished to design ELISA methods for syndecan-2 and syndecan-4, with the future aim of analyzing syndecans in large number of patient materials. Briefly, sandwich ELISAs for human syndecan-2 and syndecan-4 were designed employing monoclonal mouse antibodies as capture and commercially available polyclonal rabbit anti-syndecan as secondary antibodies. Although no quantified standard was available, relative estimation of syndecan-2 and syndecan-4 levels was possible in a small pilot material. The paper presents the ELISA method for these proteoglycans. We suggest that this may be a useful tool in the analysis of patient materials and in the detection of syndecans during proteoglycan purification.
Seidel
Carina
C
Department of Laboratory Medicine, Division of Pathology, Karolinska Institutet, Huddinge University Hospital, S-141 86 Stockholm, Sweden. carina.seidel@labmed.ki.se
Gulyás
Miklós
M
David
Guido
G
Dobra
Katalin
K
Theocharis
Achilleas D
AD
Hjerpe
Anders
A
D000818:Animals; D001201:Ascites / Q000378:metabolism; D001530:Belgium; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D008562:Membrane Glycoproteins / Q000097:blood / Q000737:chemistry*; D010996:Pleural Effusion / Q000097:blood / Q000737:chemistry; D011509:Proteoglycans / Q000097:blood / Q000737:chemistry*; D011817:Rabbits; D044967:Serum / Q000737:chemistry; D053669:Syndecan-2; D053671:Syndecan-4
D008562:Membrane Glycoproteins; D011509:Proteoglycans; C508245:SDC2 protein, human; C508304:SDC4 protein, human; C508246:Sdc2 protein, mouse; C508306:Sdc4 protein, mouse; D053671:Syndecan-4; D053669:Syndecan-2