Fungal epoxide hydrolases: new landmarks in sequence-activity space.
Epoxide hydrolases are useful catalysts for the hydrolytic kinetic resolution of epoxides, which are sought after intermediates for the synthesis of enantiopure fine chemicals. The epoxide hydrolases from Aspergillus niger and from the basidiomycetous yeasts Rhodotorula glutinis and Rhodosporidium toruloides have demonstrated potential as versatile, user friendly biocatalysts for organic synthesis. A recombinant A. niger epoxide hydrolase, produced by an overproducing A. niger strain, is already commercially available and recombinant yeast epoxide hydrolases expressed in Escherichia coli have shown excellent results. Within the vast body of activity information on the one hand and gene sequence information on the other hand, the epoxide hydrolases from the Rhodotorula spp. and A. niger stand out because we have sequence information as well as activity information for both the wild-type and recombinant forms of these enzymes.
Smit
Martha S
MS
Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, P.O. Box 339, Bloemfontein, 9300, South Africa. smitms.sci@mail.uovs.ac.za
D000595:Amino Acid Sequence; D001234:Aspergillus niger / Q000201:enzymology / Q000235:genetics; D001487:Basidiomycota / Q000201:enzymology / Q000235:genetics; D001709:Biotechnology / Q000379:methods; D002384:Catalysis; D030562:Databases, Protein; D004851:Epoxide Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004852:Epoxy Compounds / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D005658:Fungi / Q000201:enzymology* / Q000235:genetics; D006018:Glycols / Q000138:chemical synthesis / Q000737:chemistry; D007700:Kinetics; D008956:Models, Chemical; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010802:Phylogeny; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012248:Rhodotorula / Q000201:enzymology / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D004852:Epoxy Compounds; D006018:Glycols; D011994:Recombinant Proteins; D004851:Epoxide Hydrolases