title: Glucose oxidase catalysed oxidation of glucose in a dialysis membrane electrochemical reactor (D-MER).
abstract: The purpose of this work was to evaluate the effectiveness of a new Membrane Electrochemical Reactor (MER) for the production of gluconic acid by glucose oxidase (GOD) catalysed glucose oxidation. The GOD was confined against the electrode surface with a dialysis membrane. The role of the electrochemical step was to eliminate by oxidation the hydrogen peroxide that appeared as a by-product of the reaction and strongly inhibited and/or inactivated GOD. The dialysis MER gave a transformation ratio of 30% with an initial glucose concentration of around 300 mM. This result is significantly better than the maximum of 10% obtained when hydrogen peroxide was eliminated by addition of a large excess of catalase in solution, as is generally done. The D-MER also revealed unexpected properties of the enzyme kinetics, such as an oscillatory behaviour, which were discussed.
authors.affiliation: Laboratoire de Génie Chimique, CNRS UMR 5503, Basso Cambo, 5 rue Paulin Talabot, BP1301, 31106 Toulouse, France. Regine.Basseguy@ensiacet.fr
authors.forename: R
authors.initials: R
authors.lastname: Basséguy
authors.forename: K
authors.initials: K
authors.lastname: Délécouls-Servat
authors.forename: A
authors.initials: A
authors.lastname: Bergel
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D003956:Dialysis; D004563:Electrochemistry; D004566:Electrodes; D005942:Gluconates / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007700:Kinetics; D008567:Membranes, Artificial; D010084:Oxidation-Reduction
chemical_list: D005942:Gluconates; D008567:Membranes, Artificial; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose; C030691:gluconic acid