Tagged library approach to chemical genomics and proteomics.
Proteomics and chemical genomics face great challenges in the form of molecular libraries of ever increasing size and diversity requiring rapid screening, coupled with a growing number of target proteins for which complimentary molecular ligands are sought. Proteomics and chemical genomics are at a stage that requires techniques which can dramatically accelerate the discovery process. One technique that has shown great promise in accomplishing this is the tagged library approach. It entails the synthetic inclusion of an internal tag from the beginning of the synthesis. This tag adds another degree of functionality to the molecule, in addition to mere ligation, that eliminates the need for time-consuming steps downstream in the process. The tag's functional possibilities span a variety of uses including internal fluorophores, intrinsic binding motifs that enable compound identification, functionalities that play the major role in the synthesis of the ligand itself, and internal linkers that eliminate the need for lengthy 'tether effect' structure-activity relationship studies.
Mitsopoulos
Gus
G
Department of Chemistry, New York University, 29 Washington Place, New York, NY, 10003, USA.
Walsh
Daniel P
DP
Chang
Young-Tae
YT
D000818:Animals; D015698:Genomic Library; D023281:Genomics / Q000379:methods*; D006801:Humans; D009757:Nylons / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D014227:Triazines / Q000737:chemistry
D009757:Nylons; D020135:Peptide Nucleic Acids; D014227:Triazines