Detection of specific antibodies against deflagellated Salmonella Enteritidis and S. Enteritidis FliC-specific 9kDa polypeptide.
Specific antibody levels of laying hens and young chickens experimentally infected with Salmonella Enteritidis and vaccinated farm flocks were evaluated by enzyme-linked immunosorbent assays (ELISAs) with two different antigens, deflagellated S. Enteritidis whole cell (DEWC) and S. Enteritidis FliC-specific 9kDa polypeptide (SEP9). Infected laying hens excreted S. Enteritidis throughout the experimental period, and the specific antibody titers in DEWC-ELISA, were significantly higher than the uninfected group. It suggests that this DEWC-specific antibody will serve as an effective indicator of S. Enteritidis infection, especially for non-vaccinated laying flocks. SEP9-specific antibodies were detected in spray-inoculated young chickens but not in oral-inoculated young chickens. Compared with greatly high SEP9-specific antibody levels of vaccinated farm flocks, no response was observed in orally infected hens. These results indicate that S. Enteritidis discontinues expressing SEP9 once it has crossed the intestinal barrier, and that SEP9-ELISA will serve as a valuable monitoring tool for the status of S. Enteritidis vaccination on a flockwide basis, independent of stable S. Enteritidis infections.
Mizumoto
Naoe
N
Department of Veterinary Internal Medicine, Division of Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, 1-1 Gakuencho, Sakai, Osaka 599-8531, Japan.
Toyota-Hanatani
Yukiko
Y
Sasai
Kazumi
K
Tani
Hiroyuki
H
Ekawa
Tomoya
T
Ohta
Hiroaki
H
Baba
Eiichiroh
E
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial; D002645:Chickens; D002988:Cloaca / Q000382:microbiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004528:Egg Shell / Q000382:microbiology; D004530:Egg Yolk / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000382:microbiology; D005260:Female; D005408:Flagellin / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction; D011201:Poultry Diseases / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D012477:Salmonella enteritidis / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D005408:Flagellin