title: DNA microarray analysis of the correlation between gene expression patterns and acquired resistance to 5-FU/cisplatin in gastric cancer.
abstract: The mechanisms of intrinsic and/or acquired anti-cancer drug resistance have been described in in vitro resistance models, but the clinical relevance has remained undefined. We undertook a prospective study to identify correlations between gene expression and clinical resistance to 5-FU/cisplatin. We compared expression profiles from gastric cancer endoscopic biopsy specimens obtained at a chemosensitive state (partial remission after 5-FU/cisplatin) with those obtained at a refractory state (disease progression), using Affymetrix oligonucleotide microarray technology (U133A). Using 119 discriminating probes and a cross-validation approach, we were able to correctly identify the chemo-responsiveness of 7 pairs of training samples and 1 independent test pair. These exploratory data demonstrate that the gene expression profiles differ between chemosensitive and refractory state gastric cancer biopsy samples.
authors.affiliation: Research Institute and Hospital, National Cancer Center, Goyang, Gyeonggi, Republic of Korea.
authors.forename: Hark Kyun
authors.initials: HK
authors.lastname: Kim
authors.forename: Il Ju
authors.initials: IJ
authors.lastname: Choi
authors.forename: Hee Sung
authors.initials: HS
authors.lastname: Kim
authors.forename: Ju Han
authors.initials: JH
authors.lastname: Kim
authors.forename: Eugene
authors.initials: E
authors.lastname: Kim
authors.forename: In Sook
authors.initials: IS
authors.lastname: Park
authors.forename: Jong Ho
authors.initials: JH
authors.lastname: Chun
authors.forename: In-Hoo
authors.initials: IH
authors.lastname: Kim
authors.forename: Il-Jin
authors.initials: IJ
authors.lastname: Kim
authors.forename: Hio Chung
authors.initials: HC
authors.lastname: Kang
authors.forename: Jae-Hyun
authors.initials: JH
authors.lastname: Park
authors.forename: Jae-Moon
authors.initials: JM
authors.lastname: Bae
authors.forename: Jin Soo
authors.initials: JS
authors.lastname: Lee
authors.forename: Jae-Gahb
authors.initials: JG
authors.lastname: Park
mesh_terms: D000328:Adult; D000368:Aged; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D001706:Biopsy; D016000:Cluster Analysis; D018450:Disease Progression; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm; D004724:Endoscopy; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D000964:Antimetabolites, Antineoplastic; D005472:Fluorouracil