[Effects of TNF-alpha receptor blocking peptide on adjuvant arthritis in rats].
OBJECTIVE
To study the effects of TNF receptor blocking peptide on adjuvant arthritis in rats.


METHODS
The model of rat adjuvant arthritis was induced by injection of complete Freund's adjuvant. The TNF receptor blocking peptide was injected locally in the ankle. The ankle swelling, the pathologic changes in the ankle joint and the expression of IL-1 beta mRNA and TNF-alpha mRNA by peritoneal macrophages (RT-PCR) were observed.


RESULTS
The model of rat adjuvant arthritis induced by injection of complete Freund's adjuvant was similar to human rheumatoid arthritis. The treatment with TNF receptor blocking peptide for 10 days resulted in complete inhibition of joint swelling, a decrease in infiltration of inflammatory cell into joint tissue, an obvious alleviation of inflammatory pathological damages and an apparent decline of TNF-alpha mRNA and IL-1 beta mRNA of peritoneal macrophages of rats.


CONCLUSIONS
The TNF receptor blocking peptide can protect the joint from inflammatory damage induced by adjuvant arthritis by suppression of TNF-alpha and IL-1 production, thereby alleviating the pathological injury of joint and controlling effectively the clinic course of arthritis.
He
Ya-ping
YP
Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Li
Zhuo-ya
ZY
Jiang
Xiao-dan
XD
Feng
Wei
W
Xu
Yong
Y
Xiong
Ping
P
D000818:Animals; D000843:Ankle Joint / Q000473:pathology*; D001169:Arthritis, Experimental / Q000276:immunology* / Q000473:pathology; D007375:Interleukin-1 / Q000096:biosynthesis / Q000235:genetics; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008297:Male; D010455:Peptides / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D018124:Receptors, Tumor Necrosis Factor / Q000037:antagonists & inhibitors*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics
D007375:Interleukin-1; D010455:Peptides; D012333:RNA, Messenger; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha