Forskolin promotes astroglial differentiation of human central neurocytoma cells.
Human central neurocytoma is a kind of the brain tumors that are usually found in anterior part of the lateral ventricles. In this study, we established conditions that allowed proliferation of neurocytoma cells culture and analyzed characteristics of neurocytoma cells in vitro. For in vitro, a condition that used for culturing neural stem cells and contained basic fibroblast growth factor (bFGF) provided high proliferation. RT-PCR analaysis showed that nestin was found in neurocytoma cells, indicating that the neurocytomas possess neural stem cell properties. Interestingly, treatment of neurocytoma cells with forskolin increased expression of glial fibrillary acidic protein with a concomitant decrease in the nestin expression. Forskolin also induced morphological changes of neurocytoma cells to adopt an astrocyte-like phenotype. The results suggest that neurocyotma cells may have properties of multipotent neural stem cells.
Kim
Bum Jun
BJ
Department of Anatomy, School of Medicine, Ajou University, Suwon 442-749, Korea.
Kim
Sung Soo
SS
Kim
Young Im
YI
Paek
Sun Ha
SH
Lee
Young Don
YD
Suh-Kim
Haeyoung
H
D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation; D048430:Cell Shape; D005576:Colforsin / Q000494:pharmacology* / Q000627:therapeutic use*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006801:Humans; D007381:Intermediate Filament Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D018306:Neurocytoma / Q000188:drug therapy* / Q000473:pathology; D014407:Tumor Cells, Cultured
D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D016222:Fibroblast Growth Factor 2; D005576:Colforsin