Enzymatic supported synthesis of lacto-N-neotetraose using dendrimeric polyethylene glycol.
The lacto-N-neotetraose tetrasaccharide was synthesized on a new dendrimeric support, based on polyethylene glycol. Starting from 1-thio-beta-D-lactose, the trisaccharide (2-acetamido-2-deoxy-beta-D-glucopyranosyl)-(1-->3)-O-beta-D-galactopyranosyl-(1-->4)-1-thio-beta-D-glucopyranose was obtained using Neisseria meningitidis beta-(1-->3)-N-acetylglucosaminyltransferase according to a soluble synthesis approach, bound on the support and galactosylated using the milk beta-(1-->4)-galactosyl transferase to give after cleavage the tetrasaccharide lacto-N-neotetraose.
Renaudie
Laetitia
L
Laboratoire de Chimie Organique Multifonctionnelle, UMR 8614, GDR 2590 Bât 420, Université de Paris XI, F-91405 Orsay, France.
Daniellou
Richard
R
Augé
Claudine
C
Le Narvor
Christine
C
D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism*; D009345:Neisseria meningitidis / Q000201:enzymology; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011994:Recombinant Proteins / Q000378:metabolism; D013329:Structure-Activity Relationship
D009844:Oligosaccharides; D011994:Recombinant Proteins; D011092:Polyethylene Glycols; C013084:lacto-N-neotetraose; D017351:N-Acetylglucosaminyltransferases