Restriction fragment length polymorphism of L1 amplicon using Rsa 1 detects five different human papillomavirus types and their co-infections among women attending a gynaecological outpatient department.
Detection of human papillomavirus (HPV) types 6, 11, 16, 18, and 33 including co-infections among females attending gynaecological outpatient department and cancer clinics, was done by restriction fragment length polymorphism (using Rsa-1), of approximately 450bp amplicon, obtained by the amplification of the L1 region of HPV genome with consensus primers MY09/11 [Cancer Cells 7 (1989) 209]. The results were further tested with HPV type specific primers [J. Med. Virol. 29 (1989) 20]. The technique was found to be low-cost and less time consuming. The advantage of Rsa 1 over other enzymes was that it detects the five most prevalent HPV types commonly associated with warts, cervical dysplasia, and cancer.
Naqvi
Samar Husain
SH
Institute of Cytology and Preventive Oncology, Indian Council of Medical Research, Maulana Azad Medical College Campus, Bahadurshah Zafar Marg, New Delhi 110002, India.
Wajid
Saima
S
Mitra
Ardhendu B
AB
D010584:Bacteriophage phi X 174 / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D016679:Genome, Viral; D005914:Globins / Q000235:genetics; D006801:Humans; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis*; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D012749:Sexually Transmitted Diseases / Q000175:diagnosis / Q000821:virology
D017931:DNA Primers; D005914:Globins; D015252:Deoxyribonucleases, Type II Site-Specific; C040158:GTAC-specific type II deoxyribonucleases