title: Effect of site of transplantation on follicular development of human ovarian tissue transplanted into intact or castrated immunodeficient mice.
abstract: OBJECTIVE
  To study the response of human ovarian xenografts to transplantation into different sites and in different host conditions.
  
  
  METHODS
  Controlled experiment.
  
  
  METHODS
  Academic research laboratory.
  
  
  METHODS
  Donated ovarian tissue from two young women.
  
  
  METHODS
  Human ovarian cortical pieces were transplanted either under the kidney capsule or to the subcutaneous space of intact or castrated male nonobese diabetic (NOD) severe combined immune-deficient (SCID) mice. Grafts were recovered after euthanasia.
  
  
  METHODS
  Microscopic examination of histologic sections to determine proportions of growing follicles, and serum estradiol concentration measurements.
  
  
  RESULTS
  Six months after transplantation, ovarian grafts transplanted under the kidney capsule of intact male mice had significantly higher proportions of growing follicles compared with those recovered from the castrated/kidney capsule and intact/subcutaneous groups. However, no difference was detected between the intact/kidney capsule and the castrated/subcutaneous groups. Mean estradiol concentrations in serum were nonsignificantly increased in mice with ovarian grafts compared with those in mice without a graft.
  
  
  CONCLUSIONS
  Follicular development in xenotransplanted human ovarian tissue is influenced by the site of transplantation and the condition of the host.
authors.affiliation: Department of Physiology and Pharmacology, College of Veterinary Medicine, The University of Georgia, Athens, GA 30602-7389, USA.
authors.forename: Hugo
authors.initials: H
authors.lastname: Hernandez-Fonseca
authors.forename: Pablo
authors.initials: P
authors.lastname: Bosch
authors.forename: Saksiri
authors.initials: S
authors.lastname: Sirisathien
authors.forename: J David
authors.initials: JD
authors.lastname: Wininger
authors.forename: Joe B
authors.initials: JB
authors.lastname: Massey
authors.forename: Benjamin G
authors.initials: BG
authors.lastname: Brackett
mesh_terms: D000818:Animals; D004958:Estradiol / Q000097:blood; D005260:Female; D006801:Humans; D007668:Kidney / Q000601:surgery*; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009919:Orchiectomy; D009994:Osmolar Concentration; D006080:Ovarian Follicle / Q000473:pathology / Q000503:physiopathology*; D010053:Ovary / Q000637:transplantation*; D016511:Severe Combined Immunodeficiency / Q000473:pathology / Q000503:physiopathology*; D040521:Subcutaneous Tissue / Q000601:surgery*; D014183:Transplantation, Heterologous; D016042:Transplantation, Heterotopic
chemical_list: D004958:Estradiol