Viral DNA polymerase scanning and the gymnastics of Sendai virus RNA synthesis.
mRNA synthesis from nonsegmented negative-strand RNA virus (NNV) genomes is unique in tht the genome RNA is embedded in an N protein assembly (the nucleocapsid) and the viral RNA polymerase does not dissociate from the template after release of each mRNA, but rather scans the genome RNA for the next gene-start site. A revised model for NNV RNA synthesis is presented, in which RNA polymerase scanning plays a prominent role. Polymerase scanning of the template is known to occur as the viral transcriptase negotiates gene junctions without falling off the template.
Kolakofsky
Daniel
D
Department of Genetics and Microbiology, University of Geneva School of Medicine, Switzerland. Daniel.Kolakofsky@medecine.unige.ch
Le Mercier
Philippe
P
Iseni
Frédéric
F
Garcin
Dominique
D
D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000378:metabolism*; D019590:Nucleocapsid Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis*; D029082:Sendai virus / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics
D019590:Nucleocapsid Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases