Effect of ascorbic acid and acute heat exposure on heat shock protein 70 expression by young white Leghorn chickens.
Dietary ascorbic acid (AA) and heat stress (HS) affect heat shock protein 70 (hsp70) and body temperature (BT) of strain cross white Leghorn chickens. At five weeks of age, chicks fed either no supplemental AA (N-AA) or 200 mg/kg AA (AA) were subjected to either HS (42 degrees C) or maintained in control (CN, 23 degrees C) ambient temperature (T(a)) for 1 h. Body temperature (BT) was recorded for each bird before collection of heart and liver for hsp70 assay. In the CN AA-fed groups, neither the lower constitutive hsc70 nor the decreased hsp70 response to HS in the heart and liver were sex-dependent. The BT was increased by HS, but neither AA nor sex of the bird affected BT response. A diet X T(a) interaction revealed that BT of CN AA-fed chickens was lower than in N-AA-fed chickens, but BT of HS AA-fed chicks was greater than BT in HS N-AA-fed chickens. The BT and hsp70 responses were positively correlated. A lower expression of hsp70 indicated less of a stress response in the AA-fed chickens.
Mahmoud
Kamel Z
KZ
Department of Poultry Science, North Carolina State University, Box 7635, Raleigh, NC 27695-7635, USA.
Edens
F W
FW
Eisen
E J
EJ
Havenstein
G B
GB
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D015153:Blotting, Western; D002645:Chickens / Q000378:metabolism*; D003600:Cytosol / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000187:drug effects / Q000378:metabolism*; D018869:Heat-Shock Response / Q000187:drug effects / Q000502:physiology*; D006358:Hot Temperature; D008099:Liver / Q000737:chemistry; D008297:Male; D009206:Myocardium / Q000737:chemistry; D011506:Proteins / Q000032:analysis
D018840:HSP70 Heat-Shock Proteins; D011506:Proteins; D001205:Ascorbic Acid