A poliomyelitis model through mucosal infection in transgenic mice bearing human poliovirus receptor, TgPVR21.
Transgenic mice bearing the human poliovirus receptor (TgPVR) are less susceptible to oral inoculation, although they are susceptible to parenteral inoculation. We investigated the susceptibility of TgPVR 21 line [Arch. Virol. 130 (1994) 351] to poliovirus through various mucosal routes. Intranasal inoculation of a neurovirulent Mahoney strain (OM1) caused flaccid paralysis with viral replication in the central nervous system at a dose of 10(6) cell culture infectious dose (CCID50), in contrast, no paralysis following oral or intragastric inoculation of the same dose. Intranasal inoculation of a vaccine strain, Sabin 1, at 10(6) CCID50, resulted in no paralysis. Initial replication of poliovirus in the nasal cavity was confirmed by virus isolation and detection of negative-stranded replicative intermediates by RT-PCR and viral antigens using a high-sensitive immunohistochemistry and genome/transcripts by in situ hybridization. Poliovirus-specific IgG antibodies were elevated in the sera of surviving TgPVR21. This model can be used as a mucosal infection model and for differentiation of neurovirulent and attenuated poliovirus strains.
Nagata
Noriyo
N
Department of Pathology, National Institute of Infectious Diseases, Tokyo 208-0011, Japan.
Iwasaki
Takuya
T
Ami
Yasushi
Y
Sato
Yuko
Y
Hatano
Ikuyoshi
I
Harashima
Ayako
A
Suzaki
Yuriko
Y
Yoshii
Takao
T
Hashikawa
Tsutomu
T
Sata
Tetsutaro
T
Horiuchi
Yoshinobu
Y
Koike
Satoshi
S
Kurata
Takeshi
T
Nomoto
Akio
A
D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000032:analysis; D001921:Brain / Q000473:pathology / Q000821:virology; D002490:Central Nervous System / Q000473:pathology / Q000821:virology; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D008822:Mice, Transgenic; D009297:Nasal Mucosa / Q000473:pathology / Q000821:virology*; D010243:Paralysis / Q000473:pathology / Q000821:virology; D011051:Poliomyelitis / Q000097:blood / Q000473:pathology / Q000821:virology*; D017955:Poliovirus / Q000472:pathogenicity* / Q000502:physiology; D011055:Poliovirus Vaccine, Oral / Q000008:administration & dosage; D011991:Receptors, Virus / Q000235:genetics*; D013116:Spinal Cord / Q000473:pathology / Q000821:virology; D013997:Time Factors
D000914:Antibodies, Viral; D000956:Antigens, Viral; D008565:Membrane Proteins; D011055:Poliovirus Vaccine, Oral; D011991:Receptors, Virus; C066100:poliovirus receptor