Neutralization of interleukin-10 significantly enhances gamma interferon expression in peripheral blood by stimulation with Johnin purified protein derivative and by infection with Mycobacterium avium subsp. paratuberculosis in experimentally infected cattle with paratuberculosis.
Monoclonal antibody neutralization of interleukin-10 (IL-10) increased Johnin purified protein derivative-induced whole-blood gamma interferon (IFN-gamma) secretion 23-fold and also increased IFN-gamma secretion ninefold following in vitro Mycobacterium avium subsp. paratuberculosis infection of peripheral blood mononuclear cells. These results demonstrate the suppressive effect of IL-10 on immune responses to M. avium subsp. paratuberculosis infection in cattle.
Buza
Joram J
JJ
Paratuberculosis and Inflammatory Bowel Disease Research Team. Immune System Section, National Institute of Animal Health. Molecular Immunology Laboratory, National Institute of Agrobiological Sciences, 3-1-5 Kan-nondai, Tsukuba 305-0856, Japan.
Hikono
Hirokazu
H
Mori
Yasuyuki
Y
Nagata
Reiko
R
Hirayama
Sachiyo
S
Aodon-geril
Bari
Abusaleh M
AM
Shu
Yujing
Y
Tsuji
Noriko M
NM
Momotani
Eiichi
E
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000382:microbiology; D007371:Interferon-gamma / Q000378:metabolism*; D016753:Interleukin-10 / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000382:microbiology; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology* / Q000472:pathogenicity; D009500:Neutralization Tests; D010283:Paratuberculosis / Q000276:immunology* / Q000382:microbiology; D014373:Tuberculin / Q000276:immunology*; D015854:Up-Regulation
D014373:Tuberculin; D016753:Interleukin-10; D007371:Interferon-gamma