[Expression of GBD gene of Streptococcus mutans glucan binding protein A in mammalian cells].
OBJECTIVE
To evaluate the expression of recombinant plasmid pcDNA3.1/GBD of glucan binding protein of Streptococcus mutans in mammalian cells COS-7.


METHODS
Eukaryotic plasmid carrying encoding gene of GBD of Streptococcus mutans gbpA was constructed and the plasmid was introduced into COS-7 cells by Lipofectamine reagent. The transient expressed protein in COS-7 cells was detected by immunochemistry technique.


RESULTS
The positive expression was detected in plasma of the cells which were transfected with recombinant plasmid pcDNA3.1/GBD. The cells which were transfected with pcDNA3.1 were negative.


CONCLUSIONS
GBD can translate and express in COS-7 cells after transfected with recombinant plasmid pcDNA3.1/GBD. The expressed protein locates in the plasma and the protein is able to combine with anti-GbpA antibody. The expressed protein has the antigenicity and is a candidate gene vaccine.
Su
Ling-yun
LY
Dept. of Oral Medicine, College of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
Wu
Bu-ling
BL
Li
Fu-yang
FY
Lu
Qun
Q
D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D019556:COS Cells; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D003731:Dental Caries / Q000517:prevention & control; D005057:Eukaryotic Cells / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D037102:Lectins; D008322:Mammals; D010957:Plasmids / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins; D013295:Streptococcus mutans / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D019444:Vaccines, DNA
D000954:Antigens, Surface; D001426:Bacterial Proteins; D002352:Carrier Proteins; D037102:Lectins; D011994:Recombinant Proteins; D019444:Vaccines, DNA; C037937:glucan-binding proteins