Chlamydia pneumoniae induces Alzheimer-like amyloid plaques in brains of BALB/c mice.
Amyloid deposits resembling plaques found in Alzheimer's disease (AD) brains were formed in the brains of non-transgenic BALB/c mice following intranasal infection with Chlamydia pneumoniae. The mice were infected at 3 months of age with C. pneumoniae isolated from an AD brain. Infection was confirmed by light and electron microscopy in olfactory tissues of the mice. C. pneumoniae was still evident in these tissues 3 months after the initial infection indicating that a persistent infection had been established. Amyloid beta (Abeta) 1-42 immunoreactive deposits were identified in the brains of infected BALB/c mice up to 3 months post-infection with the density, size, and number of deposits increasing as the infection progressed. A subset of deposits exhibited thioflavin-s labeling. Intracellular Abeta1-42 labeling was observed in neuronal cells. Experimental induction of amyloid deposition in brains of non-transgenic BALB/c mice following infection with C. pneumoniae may be a useful model for furthering our understanding of mechanisms, linked to infection, involved in the initiation of the pathogenesis of sporadic AD.
Little
C Scott
CS
Department of Pathology, Microbiology, and Immunology, Philadelphia College of Osteopathic Medicine, 4170 City Avenue, Philadelphia, PA 19131, USA.
Hammond
Christine J
CJ
MacIntyre
Angela
A
Balin
Brian J
BJ
Appelt
Denah M
DM
D000544:Alzheimer Disease / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology*; D016229:Amyloid beta-Peptides / Q000032:analysis / Q000648:ultrastructure; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology* / Q000648:ultrastructure; D002460:Cell Line; D023521:Chlamydophila Infections / Q000473:pathology*; D016993:Chlamydophila pneumoniae / Q000502:physiology*; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000032:analysis / Q000648:ultrastructure; D058225:Plaque, Amyloid / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology* / Q000648:ultrastructure
D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42)