Apoptotic and anti-adhesion effect of ajoene, a garlic derived compound, on the murine melanoma B16F10 cells: possible role of caspase-3 and the alpha(4)beta(1) integrin.
In this study we evaluated the hypothesis that the antitumor activity of ajoene could be associated with its apoptosis-inducing effect, and with its ability to block the expression of the alpha(4)beta(1) integrin, in the murine melanoma B16F10 cells. Ajoene induced a significant reduction in B16F10 viability (IC(50)=62 microM), in a dose-dependent manner. Flow cytometric analysis showed that the cytotoxic effect of this compound was associated with caspase-3 activation. Ajoene at 25 microM altered the alpha(4)beta(1) integrin expression on B16F10, and induced a significant reduction in the adhesion of these cells to an endothelial cell monolayer.
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Eliades
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Laboratorio de Trombosis Experimental, Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas, Caracas 1020-A, Venezuela.
Apitz-Castro
Rafael
R
Cardier
José
J
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects*; D003114:Colony-Forming Units Assay; D004220:Disulfides / Q000494:pharmacology*; D005434:Flow Cytometry; D005737:Garlic / Q000737:chemistry; D005980:Glutathione Reductase / Q000037:antagonists & inhibitors; D039041:Integrin alpha4beta1 / Q000378:metabolism*; D008546:Melanoma, Experimental / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D013454:Sulfoxides; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D004220:Disulfides; D039041:Integrin alpha4beta1; D010936:Plant Extracts; D013454:Sulfoxides; C048980:ajoene; D005980:Glutathione Reductase; C505409:Casp3 protein, mouse; D053148:Caspase 3; D020169:Caspases