title: A collection of cDNAs enriched in upper cortical layers of the embryonic mouse brain.
abstract: In an attempt to elucidate the molecular basis of neuronal migration and corticogenesis, we performed subtractive hybridization of mRNAs from the upper cortical layers (layer I and upper cortical plate) against mRNAs from the remaining cerebral cortex at E15-E16. We obtained a collection of subtracted cDNA clones and analyzed their 3' UTR sequences, 47% of which correspond to EST sequences, and may represent novel products. Among the cloned sequences, we identified gene products that have not been reported in brain or in the cerebral cortex before. We examined the expression pattern of 39 subtracted clones, which was enriched in the upper layers of the cerebral cortex at embryonic stages. The expression of most clones is developmentally regulated, and especially high in embryonic and early postnatal stages. Four of the unknown clones were studied in more detail and identified as a new member of the tetraspanin superfamily, a putative RNA binding protein, a specific product of the adult dentate gyrus and a protein containing a beta-catenin repeat. We thus cloned a collection of subtracted cDNAs coding for protein products that may be involved in the development of the cerebral cortex.
authors.affiliation: IRBB/PCB and Department of Cell Biology, Faculty of Biology, University of Barcelona, 08071 Barcelona, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: García-Frigola
authors.forename: Ferran
authors.initials: F
authors.lastname: Burgaya
authors.forename: Marta
authors.initials: M
authors.lastname: Calbet
authors.forename: Guillermo
authors.initials: G
authors.lastname: López-Domènech
authors.forename: Luis
authors.initials: L
authors.lastname: de Lecea
authors.forename: Eduardo
authors.initials: E
authors.lastname: Soriano
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics*; D002465:Cell Movement / Q000235:genetics*; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D018891:Dentate Gyrus / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D005260:Female; D005333:Fetus; D015698:Genomic Library; D006133:Growth Substances / Q000235:genetics / Q000302:isolation & purification; D008565:Membrane Proteins / Q000235:genetics / Q000302:isolation & purification; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000302:isolation & purification; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018076:DNA, Complementary; D006133:Growth Substances; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins