Curcumin suppresses lipopolysaccharide-induced cyclooxygenase-2 expression by inhibiting activator protein 1 and nuclear factor kappab bindings in BV2 microglial cells.
Inflammation is a significant component of chronic neurodegenerative diseases. Cyclooxygenase-2 (COX-2) is expressed in activated microglial cells and appears to be an important source of prostaglandins during inflammatory conditions. To investigate the effect of curcumin on COX-2 gene expression in microglial cells, we treated lipopolysaccharide (LPS)-challenged BV2 microglial cells with various concentrations of curcumin. Curcumin significantly inhibited LPS-mediated induction of COX-2 expression in both mRNA and protein levels in a concentration-dependent manner. COX-2 enzyme activity was also inhibited in accordance with mRNA and protein levels. Furthermore, curcumin markedly inhibited LPS-induced nuclear factor kappaB (NF-kappaB) and activator protein 1 (AP-1) DNA bindings. These data suggest that curcumin suppresses LPS-induced COX-2 gene expression by inhibiting NF-kappaB and AP-1 DNA bindings in BV2 microglial cells.
Kang
Gu
G
Department of Pathology, College of Medicine, Kangwon National University, Chunchon, South Korea.
Kong
Pil-Jae
PJ
Yuh
Young-Jin
YJ
Lim
So-Young
SY
Yim
Sung-Vin
SV
Chun
Wanjoo
W
Kim
Sung-Soo
SS
D000818:Animals; D002478:Cells, Cultured; D003474:Curcumin / Q000737:chemistry / Q000494:pharmacology*; D051546:Cyclooxygenase 2; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000494:pharmacology; D051379:Mice; D017628:Microglia / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000187:drug effects / Q000378:metabolism; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics* / Q000378:metabolism; D011453:Prostaglandins / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000037:antagonists & inhibitors* / Q000187:drug effects / Q000378:metabolism
D008070:Lipopolysaccharides; D016328:NF-kappa B; D011453:Prostaglandins; D012333:RNA, Messenger; D018808:Transcription Factor AP-1; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; D003474:Curcumin