title: Orphan nuclear receptor Nur77 translocates to mitochondria in the early phase of apoptosis induced by synthetic chenodeoxycholic acid derivatives in human stomach cancer cell line SNU-1.
abstract: Apoptosis-inducing activity of synthetic CDCA derivatives, HS-1199 and HS-1200, on gastric cancer cell line SNU-1 cells was explored. CDCA derivatives demonstrated various apoptosis hallmarks, such as mitochondrial changes, activation of caspase, DNA fragmentation, and nuclear condensation. Importantly, the orphan receptor Nur77 (TR3) was shown to translocate from the nucleus to mitochondria at the early time points after CDCA derivatives treatment. These data support the theory that CDCA derivatives-induced apoptosis of SNU-1 gastric cancer cell lines is mediated by mitochondria and caspase, and, at least in part, by Nur77.
authors.affiliation: Department of Anatomy and Cell Biology (BK21 program), Dong-A University College of Medicine and Institute of Science, Busan, South Korea.
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Jeong
authors.forename: Joo-Sung
authors.initials: JS
authors.lastname: Park
authors.forename: Bongkyung
authors.initials: B
authors.lastname: Moon
authors.forename: Min Chan
authors.initials: MC
authors.lastname: Kim
authors.forename: Jae-Kon
authors.initials: JK
authors.lastname: Kim
authors.forename: Sungeun
authors.initials: S
authors.lastname: Lee
authors.forename: Hongsuk
authors.initials: H
authors.lastname: Suh
authors.forename: Nam Deuk
authors.initials: ND
authors.lastname: Kim
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Kim
authors.forename: Young Chul
authors.initials: YC
authors.lastname: Park
authors.forename: Young Hyun
authors.initials: YH
authors.lastname: Yoo
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002635:Chenodeoxycholic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006801:Humans; D020287:In Situ Nick-End Labeling; D007425:Intracellular Membranes / Q000187:drug effects / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology*; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; D021381:Protein Transport / Q000187:drug effects; D018160:Receptors, Cytoplasmic and Nuclear; D011987:Receptors, Steroid; D013274:Stomach Neoplasms; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; C539821:NR4A1 protein, human; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; D018160:Receptors, Cytoplasmic and Nuclear; D011987:Receptors, Steroid; D014157:Transcription Factors; D002635:Chenodeoxycholic Acid