title: Dissemination of CTX-M type beta-lactamases in Enterobacteriaceae isolates in the People's Republic of China.
abstract: Previously there have been a number of reports of extended spectrum beta-lactamase (ESBL) producing isolates of the family Enterobacteriaceae in Asia. We first reported the occurrence of bla(CTX-M) in Guangzhou, China, subsequently there have been reports of bla(CTX-M) from a number of other south Asian countries. Initial surveillance study data suggested that bla(CTX-M) might be widely distributed in China. This study examines the type of bla(CTX-M) occurring in other major population centres in China. Initial disk diffusion method susceptibility testing (NCCLS) selected ESBL producing Escherichia coli and Klebsiella pneumoniae isolates from Beijing and near Wuhan, PRC. After screening in both China and the UK, 13 isolates producing CTX-M ESBLs were identified and studied, 11 also produced TEM-1, and 4 also produced SHV-1. Sequence analysis of the bla(CTX-M) containing isolates revealed these isolates contained two different bla(CTX-M), three with bla(CTX-M-3) and 10 with bla(CTX-M-14). After comparison with other previously published studies in the English language, we conclude that the most prevalent bla(CTX-M) so far reported in Asia are bla(CTX-M-14) and bla(CTX-M-3).
authors.affiliation: Division of Immunobiology and Infection, The Medical School, University of Birmingham, Vincent Drive, Edgbaston, Birmingham, B15 2TT, UK.
authors.forename: C J
authors.initials: CJ
authors.lastname: Munday
authors.forename: J
authors.initials: J
authors.lastname: Xiong
authors.forename: C
authors.initials: C
authors.lastname: Li
authors.forename: D
authors.initials: D
authors.lastname: Shen
authors.forename: P M
authors.initials: PM
authors.lastname: Hawkey
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002681:China / Q000453:epidemiology; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004755:Enterobacteriaceae / Q000145:classification / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D004756:Enterobacteriaceae Infections / Q000453:epidemiology / Q000382:microbiology; D022761:Gene Transfer, Horizontal; D008826:Microbial Sensitivity Tests; D010957:Plasmids; D019105:Random Amplified Polymorphic DNA Technique; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D047090:beta-Lactams; C456080:beta-lactamase CTX-M-14; C417271:beta-lactamase CTX-M-3; D001618:beta-Lactamases