title: Glucosylceramides in Colletotrichum gloeosporioides are involved in the differentiation of conidia into mycelial cells.
abstract: Glucosylceramides (GlcCer) were extracted from the plant pathogen Colletotrichum gloeosporioides and purified by several chromatographic steps. By using electrospray ionization mass spectrometry and nuclear magnetic resonance, GlcCer from C. gloeosporioides were identified as N-2'-hydroxyoctadecanoyl-1-beta-D-glucopyranosyl-9-methyl-4,8-sphingadienine and N-2'-hydroxyoctadecenoyl-1-beta-D-glucopyranosyl-9-methyl-4,8-sphingadienine. Monoclonal antibodies against these structures were produced and used as tools for the evaluation of the role of GlcCer in the morphological transition of C. gloeosporioides. In the presence of antibodies to GlcCer, the differentiation of conidia into mycelia was blocked. Since GlcCer is present in several plant pathogens, the inhibitory activity of external ligands recognizing these structures may be applicable in other models of fungal infections.
authors.affiliation: Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Bloco I, Cidade Universitária, Rio de Janeiro 21941-590, Brazil.
authors.forename: André F C
authors.initials: AF
authors.lastname: da Silva
authors.forename: Marcio L
authors.initials: ML
authors.lastname: Rodrigues
authors.forename: Sandra E
authors.initials: SE
authors.lastname: Farias
authors.forename: Igor C
authors.initials: IC
authors.lastname: Almeida
authors.forename: Márcia R
authors.initials: MR
authors.lastname: Pinto
authors.forename: Eliana
authors.initials: E
authors.lastname: Barreto-Bergter
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D002554:Cerebrosides / Q000276:immunology / Q000302:isolation & purification; D020231:Colletotrichum / Q000737:chemistry* / Q000166:cytology* / Q000254:growth & development; D005963:Glucosylceramides / Q000276:immunology / Q000302:isolation & purification* / Q000502:physiology*; D009682:Magnetic Resonance Spectroscopy; D008856:Microscopy, Fluorescence; D009024:Morphogenesis; D025282:Mycelium / Q000166:cytology; D021241:Spectrometry, Mass, Electrospray Ionization; D013172:Spores, Fungal / Q000166:cytology
chemical_list: D000911:Antibodies, Monoclonal; D002554:Cerebrosides; D005963:Glucosylceramides; C019425:N-2'-hydroxy-3'-trans-octadecenoyl-1-o-beta-D-glucosyl- 9-methyl-cis-4,x-8-sphingadienine