Effect of hyperosmotic shock on phosphoenolpyruvate carboxykinase gene expression and gluconeogenic activity in the crab muscle.
Chasmagnathus granulata phosphoenolpyruvate carboxykinase (PEPCK) cDNA from jaw muscle was cloned and sequenced, showing a specific domain to bind phosphoenolpyruvate in addition to the kinase-1 and kinase-2 motifs to bind guanosine triphosphate (GTP) and Mg(2+), respectively, specific for all PEPCKs. In the kinase-1 motifs the GK was changed to RK. The first 19 amino acids of the putative enzyme contain hydrophobic amino acids and hydroxylated residues specific to a mitochondrial type signal. The PEPCK is expressed in hepatopancreas, muscles, nervous system, heart, and gills. Hyperosmotic stress for 24 h increased the PEPCK mRNA level, gluconeogenic and PEPCK activities in muscle.
Schein
Vanessa
V
Department of Physiology, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, 90050-170, Porto Alegre, RS, Brazil.
Waché
Yann
Y
Etges
Rodrigo
R
Kucharski
Luiz Carlos
LC
van Wormhoudt
Alain
A
Da Silva
Roselis S M
RS
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D003386:Brachyura; D004790:Enzyme Induction; D005943:Gluconeogenesis; D008297:Male; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism*; D009997:Osmotic Pressure; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014018:Tissue Distribution
D012333:RNA, Messenger; D010729:Phosphoenolpyruvate Carboxykinase (GTP)