Stability improvement of immobilized Candida antarctica lipase B in an organic medium under microwave radiation.
The influence of microwave heating on the stability of immobilized Candida antarctica lipase B was studied at 100 degrees in an organic medium. The microwave radiation was carried out before enzymatic reaction (storage conditions) or during the enzymatic catalysis (use conditions). In both cases, enzymatic stability was higher under microwave heating than under conventional thermal heating, in strictly identical operating conditions. Furthermore, the gain of enzymatic stability under microwave heating appears to be higher in a more polar solvent, which interacts strongly with the microwave field. Our results suggest that microwave radiation has an effect, not related to temperature, on the process of enzymatic inactivation.
Réjasse
Barbara
B
Laboratoire de Biotechnologies et Chimie Bioorganique, CNRS FRE-2766, UFR Sciences Fondamentales et Sciences pour l'Ingénieur, Bâtiment Marie Curie, Université de la Rochelle, F-17042, La Rochelle cedex 1, France.
Lamare
Sylvain
S
Legoy
Marie-Dominique
MD
Besson
Thierry
T
D000440:Butanols / Q000737:chemistry; D002175:Candida / Q000201:enzymology*; D002384:Catalysis; D004795:Enzyme Stability / Q000528:radiation effects; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000528:radiation effects; D005656:Fungal Proteins; D007700:Kinetics; D008049:Lipase / Q000737:chemistry* / Q000528:radiation effects; D008872:Microwaves; D009930:Organic Chemicals / Q000737:chemistry*; D013696:Temperature; D013816:Thermodynamics
D000440:Butanols; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D009930:Organic Chemicals; D008049:Lipase; C431349:lipase B, Candida antarctica