title: Chemical modification of glucose oxidase: possible formation of molten globule-like intermediate structure.
abstract: Chemical modification of lysine residues in glucose oxidase was carried out using citraconic anhydride. Modification brought about changes in the kinetic properties of the enzyme as evident by substantial lowering of V(max) and K(m). Enhancement of tryptophan fluorescence was observed with a dramatic change in its pH dependence due to modification. Near- and far-UV circular dichroism spectra of the native and modified forms suggested formation of molten globule-like structures, further supported by 8-anilino-1-naphthalenesulfonic acid fluorescence which indicated higher exposure of hydrophobic residues as a result of chemical modification.
authors.affiliation: Department of Biochemistry, Faculty of Basic Sciences, Tarbiat Modarres University, P.O. Box 14115-175, Tehran, Iran.
authors.forename: Saman
authors.initials: S
authors.lastname: Hosseinkhani
authors.forename: Bijan
authors.initials: B
authors.lastname: Ranjbar
authors.forename: Hossein
authors.initials: H
authors.lastname: Naderi-Manesh
authors.forename: Mohsen
authors.initials: M
authors.lastname: Nemat-Gorgani
mesh_terms: D000817:Anilino Naphthalenesulfonates; D002942:Circular Dichroism; D002949:Citraconic Anhydrides / Q000737:chemistry; D005453:Fluorescence; D005949:Glucose Oxidase / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D011487:Protein Conformation; D014364:Tryptophan / Q000737:chemistry
chemical_list: D000817:Anilino Naphthalenesulfonates; D002949:Citraconic Anhydrides; C027132:1-anilino-8-naphthalenesulfonate; D014364:Tryptophan; D005949:Glucose Oxidase