[A novel cDNA array method for detecting the chimeric genes in human leukemia].
Advanced molecular diagnostics in hematological malignancy have provided us with a broad assortment of new assays and techniques. We have developed a simple cDNA microarray method for detecting the chimeric genes in human leukemia. The method, based on the biotin-avidine reaction, is performed with color development. The oligotip we have developed is indicated by simple and highly sensitive data. By analyzing with this method, we can obtain useful information about the subtype of chimeric genes in leukemia.
Funato
Tadao
T
Division of Molecular Diagnostics, Tohoku University, School of Medicine, Sendai 980-8574.
D001360:Avidin; D001710:Biotin; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D007938:Leukemia / Q000175:diagnosis* / Q000235:genetics*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D014407:Tumor Cells, Cultured
D011993:Recombinant Fusion Proteins; D001360:Avidin; D001710:Biotin