Two-dimensional diversity: screening human cDNA phage display libraries with a random diversity probe for the display cloning of phosphotyrosine binding domains.
A random phosphopeptide probe (bio-pYZZZ) has been used for the isolation and identification of multiple SH2 domains from human cDNA-displaying phage libraries. In addition, on-phage analysis and quantification of binding affinities for these phage-displayed proteins has shown them to be functional domains, retaining the same characteristics as in their native state.
Videlock
Elizabeth J
EJ
Department of Chemistry, Yale University, New Haven, Connecticut 06520, USA.
Chung
Victor K
VK
Mohan
Michael A
MA
Strok
Timothy M
TM
Austin
David J
DJ
D003001:Cloning, Molecular; D003090:Coliphages / Q000235:genetics; D015723:Gene Library; D006801:Humans; D008024:Ligands; D015394:Molecular Structure; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D015398:Signal Transduction; D018909:src Homology Domains
D008024:Ligands; D010446:Peptide Fragments; D019000:Phosphotyrosine