title: Evaluation of anesthesia effects on [18F]FDG uptake in mouse brain and heart using small animal PET.
abstract: This study evaluates effects of anesthesia on (18)F-FDG (FDG) uptake in mouse brain and heart to establish the basic conditions of small animal PET imaging. Prior to FDG injection, 12 mice were anesthetized with isoflurane gas; 11 mice were anesthetized with an intraperitoneal injection of a ketamine/xylazine mixture; and 11 mice were awake. In isoflurane and ketamine/xylazine conditions, FDG brain uptake (%ID/g) was significantly lower than in controls. Conversely, in the isoflurane condition, %ID/g in heart was significantly higher than in controls, whereas heart uptake in ketamine/xylazine mice was significantly lower. Results suggest that anesthesia impedes FDG uptake in mouse brain and affects FDG uptake in heart; however, the effects in the brain and heart differ depending on the type of anesthesia used.
authors.affiliation: Molecular Imaging Branch, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892, USA. htoyama@fujita-hu.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Toyama
authors.forename: Masanori
authors.initials: M
authors.lastname: Ichise
authors.forename: Jeih-San
authors.initials: JS
authors.lastname: Liow
authors.forename: Douglass C
authors.initials: DC
authors.lastname: Vines
authors.forename: Nicholas M
authors.initials: NM
authors.lastname: Seneca
authors.forename: Kendra J
authors.initials: KJ
authors.lastname: Modell
authors.forename: Jurgen
authors.initials: J
authors.lastname: Seidel
authors.forename: Michael V
authors.initials: MV
authors.lastname: Green
authors.forename: Robert B
authors.initials: RB
authors.lastname: Innis
mesh_terms: D000758:Anesthesia / Q000379:methods*; D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging / Q000187:drug effects / Q000378:metabolism*; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics*; D006321:Heart / Q000000981:diagnostic imaging / Q000187:drug effects; D007530:Isoflurane / Q000494:pharmacology; D007649:Ketamine / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009206:Myocardium / Q000378:metabolism*; D049268:Positron-Emission Tomography / Q000379:methods*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D014991:Xylazine / Q000494:pharmacology
chemical_list: D000777:Anesthetics; D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18; D014991:Xylazine; D007649:Ketamine; D007530:Isoflurane