title: Rhamnolipid biosurfactants produced by Renibacterium salmoninarum 27BN during growth on n-hexadecane.
abstract: A new strain Renibacterium salmoninarum 27BN was isolated for its capacity to utilize n-hexadecane as sole substrate. Growth on n-hexadecane was accompanied with the production of glycolipid surface active substances detected by surface pressure lowering and emulsifying activity. Glycolipid detection by thin layer chromatography and infrared spectra analyses showed for the first time that Renibacterium salmoninarum 27BN secretes the two rhamnolipids RLL and RRLL typical for Pseudomonas aeruginosa. Growth of Renibacterium salmoninarum 27BN on n-hexadecane depended on the bioavailability of the substrate and the secreted rhamnolipids appeared to be efficient in increasing hexadecane availability for the cells.
authors.affiliation: Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev str, bl 26, 1113 Sofia, Bulgaria. nhrist@yahoo.com
authors.forename: Nelly
authors.initials: N
authors.lastname: Christova
authors.forename: Borjana
authors.initials: B
authors.lastname: Tuleva
authors.forename: Zdravko
authors.initials: Z
authors.lastname: Lalchev
authors.forename: Albena
authors.initials: A
authors.lastname: Jordanova
authors.forename: Bojidar
authors.initials: B
authors.lastname: Jordanov
mesh_terms: D000473:Alkanes / Q000494:pharmacology*; D006017:Glycolipids / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008835:Micrococcaceae / Q000737:chemistry* / Q000187:drug effects / Q000254:growth & development; D013055:Spectrophotometry, Infrared; D013501:Surface-Active Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000473:Alkanes; D006017:Glycolipids; D013501:Surface-Active Agents; C418382:rhamnolipid; C007932:n-hexadecane