Arsenicicoccus bolidensis gen. nov., sp. nov., a novel actinomycete isolated from contaminated lake sediment.
An unknown Gram-positive, catalase-positive, facultatively anaerobic, non-spore-forming, coccus-shaped bacterium originating from sediment was characterized using phenotypic, molecular chemical and molecular phylogenetic methods. Chemical studies revealed the presence of a cell-wall murein based on LL-diaminopimelic acid (type LL-Dpm-glycine(1)), a complex mixture of saturated, monounsaturated and iso- and anteiso-methyl-branched, non-hydroxylated, long-chain cellular fatty acids and tetrahydrogenated menaquinones with eight isoprene units [MK-8(H(4))] as the major respiratory lipoquinone. This combination of characteristics somewhat resembled members of the suborder Micrococcineae, but did not correspond to any currently described species. Comparative 16S rRNA gene sequencing confirmed that the unidentified coccus-shaped organism is a member of the Actinobacteria and represents a hitherto-unknown subline related to, albeit different from, a number of taxa including Intrasporangium, Janibacter, Terrabacter, Terracoccus and Ornithinicoccus. Based on phenotypic and phylogenetic considerations, it is proposed that the unknown bacterium originating from lake sediment be classified as a new genus and species, Arsenicicoccus bolidensis gen. nov., sp. nov. (type strain CCUG 47306(T)=DSM 15745(T)).
Collins
Matthew D
MD
School of Food Biosciences, University of Reading, Whiteknights, Reading RG6 6AP, UK.
Routh
Joyanto
J
Department of Geology and Geochemistry, Stockholm University, Stockholm, Sweden.
Saraswathy
Ambujom
A
Department of Geology and Geochemistry, Stockholm University, Stockholm, Sweden.
Lawson
Paul A
PA
School of Food Biosciences, University of Reading, Whiteknights, Reading RG6 6AP, UK.
Schumann
Peter
P
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany.
Welinder-Olsson
Christina
C
Culture Collection, Department of Clinical Bacteriology, University of Göteborg, Göteborg, Sweden.
Falsen
Enevold
E
Culture Collection, Department of Clinical Bacteriology, University of Göteborg, Göteborg, Sweden.
D000192:Actinomycetales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D005618:Fresh Water / Q000382:microbiology; D019015:Geologic Sediments / Q000382:microbiology; D006094:Gram-Positive Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D014876:Water Pollution