title: Use of whole blood dried on filter paper for detection and genotyping of measles virus.
abstract: PCR, and two different ELISAs were used to detect measles virus on blood dried on filter paper samples from Equatorial Guinea. Sensitivity was 40% by PCR, 57.1% by indirect ELISA and 86.7% by micro chain capture ELISA. Genotype B3 was found in two positive samples by PCR.
authors.affiliation: Centro Nacional de Microbiología, Instituto de Salud Carlos III, Carretera de Majadahonda-Pozuelo s/n, 28220 Majadahonda, Madrid, Spain. mmosquera@isciii.es
authors.forename: María del Mar
authors.initials: Mdel M
authors.lastname: Mosquera
authors.forename: Juan E
authors.initials: JE
authors.lastname: Echevarría
authors.forename: Sabino
authors.initials: S
authors.lastname: Puente
authors.forename: Félix
authors.initials: F
authors.lastname: Lahulla
authors.forename: Fernando
authors.initials: F
authors.lastname: de Ory
mesh_terms: D001800:Blood Specimen Collection / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005838:Genotype; D006801:Humans; D007223:Infant; D008459:Measles virus / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity