Evidences for the formation of bisbenzamidine-heme complexes in cell-free systems.
Infrared and colorimetry data suggest that bisbenzamidines connected by various rigid or flexible linkers are able to interact with heme in cell-free systems. At pH 5.0 the inhibition of formation of beta-hematin could be ascertained by infrared spectroscopy whereas at pH 7.0 the interaction yielded insoluble complexes for which a sandwich-type structure of stoichiometry 2:1, heme-drug, is tentatively proposed.
Mayence
Annie
A
Xavier University of Louisiana, College of Pharmacy, Division of Basic Pharmaceutical Sciences, 1 Drexel Drive, New Orleans, LA 70125, USA.
Vanden Eynde
Jean Jacques
JJ
Huang
Tien L
TL
D000818:Animals; D000962:Antimalarials / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D001550:Benzamidines / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D002474:Cell-Free System; D003124:Colorimetry / Q000379:methods; D006418:Heme / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D010963:Plasmodium falciparum / Q000187:drug effects / Q000378:metabolism
D000962:Antimalarials; D001550:Benzamidines; D006418:Heme