title: Induction of apoptosis and necrosis in lymphocytes by the cis-Pt(II) complex of 3-aminoflavone in comparison with cis-DDP.
abstract: cis-Diamminedichloroplatinum(II) (cis-DDP) is one of the most widely administrated antitumor drugs. However, the use of cis-DDP is severely limited because of its toxic side effects. Therefore, efforts are concentrated on the development of improved platinum compounds with a broader activity spectrum and effectiveness in chemotherapy, but lower toxicity. Beneficial properties of flavonoids, e.g. their antitumor activity, encouraged scientists to synthesize cis-bis(3-aminoflavone)dichloroplatinum(II). Abilities of these compounds to induce apoptosis and necrosis were compared by use of trypan blue, fluorochrome staining (Hoechst 33258/propidium iodide double staining) and TUNEL assays. The cytotoxicity was evaluated by MTT. The results obtained show that the cis-Pt(II) complex of 3-aminoflavone is less toxic than cis-DDP. However, the former compound has a faster rate of apoptosis induction in lymphocytes than the latter. The cis-Pt(II) complex of 3-aminoflavone induces apoptosis in normal lymphocytes to a lesser degree and could be a potential antitumor drug.
authors.affiliation: Department of Cytogenetics and Plant Molecular Biology, University of Lodz, ul. Banacha 12/16, 90-237 Lodz, Poland. kosbea@biol.uni.lodz.pl
authors.forename: Beata
authors.initials: B
authors.lastname: Kosmider
authors.forename: Regina
authors.initials: R
authors.lastname: Osiecka
authors.forename: Ewa
authors.initials: E
authors.lastname: Ciesielska
authors.forename: Leszek
authors.initials: L
authors.lastname: Szmigiero
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Zyner
authors.forename: Justyn
authors.initials: J
authors.lastname: Ochocki
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002945:Cisplatin / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D020287:In Situ Nick-End Labeling; D008856:Microscopy, Fluorescence; D009336:Necrosis
chemical_list: C488624:3-aminoflavone; D005419:Flavonoids; D002945:Cisplatin