Cross-reactivity and inhibition of haemolysis by polyclonal antibodies raised against St II, a cytolysin from the sea anemone Stichodactyla helianthus.
The immunogenicity of sticholysin II (St II), a pore-forming polypeptide from the sea anemone Stichodactyla helianthus, was studied in rabbits using two adjuvants, Freund's and aluminium hydroxide. High titres of antibodies were raised against St II with Freund's adjuvant (FA). The structural homology between sticholysins I and II was also revealed by cross-reactivity assays. Since the oil constituent of FA neutralized the St II haemolytic activity, immunizations with St II-Freund's emulsions were carried out with the inactivated cytolysin. Purified anti-St II IgG also neutralized the St II haemolytic activity.
Cristina Pico
María
M
Centro de Estudio de Proteínas, Facultad de Biología, Universidad de La Habana, Calle 25 No. 455, entre J e I. Vedado, Ciudad Habana 10400, Cuba. mcpico@fbio.uh.cu
Basulto
Ariel
A
del Monte
Alberto
A
Hidalgo
Abdel
A
Eliana Lanio
María
M
Alvarez
Carlos
C
Felicó
Eric
E
Otero
Anselmo
A
D000536:Aluminum Hydroxide / Q000276:immunology / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002846:Chromatography, Affinity; D003064:Cnidarian Venoms / Q000276:immunology* / Q000378:metabolism; D003429:Cross Reactions / Q000276:immunology; D003603:Cytotoxins / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005620:Freund's Adjuvant / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects*; D008074:Lipoproteins / Q000097:blood / Q000378:metabolism; D011817:Rabbits; D012615:Sea Anemones; D012799:Sialyltransferases / Q000276:immunology* / Q000378:metabolism; D013053:Spectrophotometry
D000906:Antibodies; D003064:Cnidarian Venoms; D003603:Cytotoxins; D008074:Lipoproteins; D000536:Aluminum Hydroxide; D005620:Freund's Adjuvant; D012799:Sialyltransferases; C043662:alpha-N-acetylneuraminate alpha-2,8-sialyltransferase