title: [Characterization of Notch gene involved in genetic regulatory networks in dental pulp stem cells].
abstract: OBJECTIVE
  To investigate the characterization of Notch gene involved in genetic regulatory networks in dental pulp stem cells.
  
  
  METHODS
  The pulp tissue was separated from mouse teeth and digested by collagenase type I. Single-cell suspensions of dental pulp were seeded into 6-well plates with alpha modification of Eagle's medium supplemented with ES cell qualified Fetal Bovine Serum. Colony-forming efficiency was assessed in 14ds culture. Transcripts for Notch were detected by reverse transcription-PCR by using total RNA isolated from cells.
  
  
  RESULTS
  There were clonogenic cells in dental pulp cell and the incidence of colony-forming cells derived from mouse dental pulp cells was 1.6-2.5 colonies/10(4) plate. Mouse-specific Notch mRNA expressed in colony-forming cells.
  
  
  CONCLUSIONS
  Notch mRNA expressing in colony-forming cells provided a more detailed understanding of mouse dental pulp stem cell biology.
authors.affiliation: Dept. of Oral Medicine, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Lu
authors.forename: Bu-ling
authors.initials: BL
authors.lastname: Wu
authors.forename: Ji-shu
authors.initials: JS
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Han
authors.forename: Xue-dong
authors.initials: XD
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology* / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000235:genetics*; D051880:Receptors, Notch; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D051880:Receptors, Notch