title: Detection of t(14;18) in follicular lymphoma by dual-color fluorescence in situ hybridization on paraffin-embedded tissue sections.
abstract: We studied the incidence of t(14;18)(q32;q21) in 54 patients with follicular lymphoma (FL) by dual-color fluorescence in situ hybridization on paraffin-embedded tissue sections (tissue-FISH) using probes for BCL2 and immunoglobulin heavy chain (IGH) genes. The t(14;18) was detected in 28 (56%) of 50 patients, who were successfully analyzed. On the other hand, BCL2 protein expression was detected in 45 (83%) of 54 patients evaluated by immunohistochemical staining. There was a discrepancy between t(14;18) and BCL2 expression. The absence of t(14;18) was associated with disease-free survival (P=0.02). There was no statistical difference, however, in overall survival and failure-free survival between the t(14;18)-positive and -negative groups. Tissue-FISH should be of great value to detect t(14;18) in FL because this method enabled us to demonstrate specifically t(14;18)-positive individual cells in neoplastic follicles on paraffin-embedded tissue sections. Furthermore, tissue-FISH can be applied to small specimens obtained from endoscopic biopsy or specimens obtained more than 10 years ago. We validated the usefulness of tissue-FISH as a diagnostic procedure and retrospective meta-analysis for malignant lymphoma.
authors.affiliation: Department of Medicine, Division of Hematology and Oncology, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji, Kamigyo-ku, Kyoto 602-0841, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Kenichi
authors.initials: K
authors.lastname: Nomura
authors.forename: Sawako
authors.initials: S
authors.lastname: Matsumoto
authors.forename: Kyoji
authors.initials: K
authors.lastname: Ueda
authors.forename: Mitsushige
authors.initials: M
authors.lastname: Nakao
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nishida
authors.forename: Hideaki
authors.initials: H
authors.lastname: Sakabe
authors.forename: Shohei
authors.initials: S
authors.lastname: Yokota
authors.forename: Shigeo
authors.initials: S
authors.lastname: Horiike
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Nakamine
authors.forename: Shigeo
authors.initials: S
authors.lastname: Nakamura
authors.forename: Masafumi
authors.initials: M
authors.lastname: Taniwaki
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007621:Karyotyping; D008224:Lymphoma, Follicular / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016612:Paraffin Embedding; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D014178:Translocation, Genetic
chemical_list: D007143:Immunoglobulin Heavy Chains; D019253:Proto-Oncogene Proteins c-bcl-2