title: Primary cilia of human endothelial cells disassemble under laminar shear stress.
abstract: We identified primary cilia and centrosomes in cultured human umbilical vein endothelial cells (HUVEC) by antibodies to acetyl-alpha-tubulin and capillary morphogenesis gene-1 product (CMG-1), a human homologue of the intraflagellar transport (IFT) protein IFT-71 in Chlamydomonas. CMG-1 was present in particles along primary cilia of HUVEC at interphase and around the oldest basal body/centriole at interphase and mitosis. To study the response of primary cilia and centrosomes to mechanical stimuli, we exposed cultured HUVEC to laminar shear stress (LSS). Under LSS, all primary cilia disassembled, and centrosomes were deprived of CMG-1. We conclude that the exposure to LSS ends the IFT in cultured endothelial cells.
authors.affiliation: Center for Anatomy and Functional Morphology, Mount Sinai School of Medicine, 1 Gustave L. Levy Pl., Box 1007, New York, NY 10029, USA.
authors.forename: Carlo
authors.initials: C
authors.lastname: Iomini
authors.forename: Karla
authors.initials: K
authors.lastname: Tejada
authors.forename: Wenjun
authors.initials: W
authors.lastname: Mo
authors.forename: Heikki
authors.initials: H
authors.lastname: Vaananen
authors.forename: Gianni
authors.initials: G
authors.lastname: Piperno
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D002478:Cells, Cultured; D018385:Centrosome / Q000378:metabolism; D002696:Chlamydomonas / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D002923:Cilia / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology; D005407:Flagella / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D011506:Proteins / Q000378:metabolism; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D033081:Shear Strength; D013314:Stress, Mechanical; D050396:TRPP Cation Channels; D014404:Tubulin / Q000737:chemistry / Q000378:metabolism
chemical_list: D003598:Cytoskeletal Proteins; D011506:Proteins; D015800:Protozoan Proteins; D050396:TRPP Cation Channels; D014404:Tubulin; C490080:IFT74 protein, human