SCC-112, a novel cell cycle-regulated molecule, exhibits reduced expression in human renal carcinomas.
We report here the identification and an initial characterization of a novel cell cycle-regulated molecule, SCC-112. SCC-112 cDNA (6744 bp) encodes a longest open reading frame (ORF) comprised of 1297 amino acids, representing a approximately 150-kDa nuclear protein. SCC-112 mRNA and protein levels were relatively high during the G2/M phase of the cell cycle in MDA-MB 435 breast cancer cells. Transient expression of SCC-112 cDNA in COS-1 cells led to an increase in the number of cells in sub-G1 phase and enhanced activity of caspase-3, a downstream effector of apoptosis. Stable transfection of SCC-112 cDNA in MDA-MB 231 breast cancer cells also led to an increase in the number of cells in sub-G1 phase ( approximately 2-3-fold), indicative of apoptosis. The examination of the paired sets of human normal and tumor tissues revealed that the SCC-112 mRNA level was significantly high in normal breast and kidney tissues as compared to the corresponding primary tumor tissues (P<0.0001; breast, n=50, and kidney, n=20). Consistent with these observations, SCC-112 protein expression (150 kDa) was high in a majority of the normal renal tissues examined as compared to the matched renal tumor tissues (67%, 1.2-fold to>10-fold, n=18). Taken together, these findings suggest that the SCC-112 gene expression is likely to be associated with normal cell growth and proliferation.
Kumar
Deepak
D
Department of Radiation Medicine, Lombardi Cancer Center, Georgetown University, E208, Research Building, 3970 Reservoir Road, NW, Washington, DC 20007, USA.
Sakabe
Isamu
I
Patel
Sonal
S
Zhang
Ying
Y
Ahmad
Imran
I
Gehan
Edmund A
EA
Whiteside
Theresa L
TL
Kasid
Usha
U
D000328:Adult; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology; D019556:COS Cells; D002453:Cell Cycle / Q000502:physiology*; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005260:Female; D016195:G2 Phase / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014162:Transfection
D018076:DNA, Complementary; D009687:Nuclear Proteins; C486457:PDS5A protein, human; D012333:RNA, Messenger