Electrochemical studies of kanamycin immobilization on self-assembled monolayer and interaction with DNA.
The electrochemical characteristics of kanamycin onto self-assembled monolayer (SAM) modified gold electrode (SAM/Au) is investigated by cyclic voltammetry. In the potential range 0-0.6 V, Cu(II) yields a pair of stable redox waves at the bare gold electrode. E(pa) is located at 0.189 V and E(pc) at 0.254 V. In contrast, Cu(II) is reduced at a more positive potential and a decreasing current at the kanamycin SAM/Au electrode. Cu(II) and kanamycin can form a stoichiometry complex with chemical ratio of 2:1. The interaction of Cu(II)-kanamycin complex with calf thymus DNA is also studied in the solution. And the interactive mode between Cu(II)-kanamycin complex and DNA is verified by the fluorescence method. Binding constants (K) of the Cu(II)-kanamycin complex to DNA and binding site size (s) are calculated from voltammetric data and equal to 1.5 x 10(7) l/mol and 4 bp, respectively.
Lu
Xiaoquan
X
Department of Chemistry, Northwest Normal University, Anning Eastern Road 805, Lanzhou 730070, Gansu, PR China. xqlu88@nwnu.edu.cn
Zhang
Min
M
Kang
Jingwan
J
Wang
Xiaoqiang
X
Zhuo
Lin
L
Liu
Hongde
H
D000818:Animals; D002417:Cattle; D003300:Copper / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D007612:Kanamycin / Q000737:chemistry*; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence; D017186:Titrimetry
D007612:Kanamycin; D006046:Gold; D003300:Copper; D004247:DNA