Improved photostability and fluorescence properties through polyfluorination of a cyanine dye.
[structure: see text] A polyfluorinated cyanine dye has been synthesized and characterized. Compared with the nonfluorinated analogue, the dye exhibits significantly reduced aggregation in aqueous media, enhanced fluorescence quantum yield, greater resistance to photobleaching upon direct irradiation, and reduced reactivity toward singlet oxygen. All of these properties are favorable for use of cyanine dyes as fluorescent labels and point toward fluorination as a general strategy for improving performance in imaging applications.
Renikuntla
Babu Rao
BR
Department of Chemistry, Molecular Biosensor and Imaging Center, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213-3890, USA.
Rose
Heather C
HC
Eldo
Joby
J
Waggoner
Alan S
AS
Armitage
Bruce A
BA
D002232:Carbocyanines / Q000138:chemical synthesis / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D005461:Fluorine / Q000737:chemistry*; D008027:Light; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D038761:Photobleaching; D026082:Singlet Oxygen / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D002232:Carbocyanines; D005456:Fluorescent Dyes; D026082:Singlet Oxygen; D005461:Fluorine