title: Novel expression patterns of phosphatidylinositol 3-hydroxy kinase in nodulated Medicago spp. plants.
abstract: A cDNA clone encoding a phosphatidylinositol 3-kinase (PtdIns 3-kinase) has been characterized from Medicago truncatula, Mtpi3k, that is highly homologous to their counterparts from soybean (over 84%). The results suggest the presence of at least two genes coding PtdIns 3-kinases in M. truncatula. Mtpi3k transcript levels increased in nodules, compared with non-infected roots. Strikingly, Mtpi3k mRNA accumulated in young elongating stems at higher levels than that observed in other organs. Enhanced transcription of genes coding PtdIns 3-kinases might occur in tissues experiencing a high degree of vesicle trafficking and cell elongation.
authors.affiliation: Laboratory of Molecular Biology, Department of Plant Sciences, Wageningen Agricultural University, Dreijenlaan 3, 6703 HA Wageningen,The Netherlands. luisehernandez@uam.es
authors.forename: Luis E
authors.initials: LE
authors.lastname: Hernández
authors.forename: Carolina
authors.initials: C
authors.lastname: Escobar
authors.forename: Bjørn K
authors.initials: BK
authors.lastname: Drøbak
authors.forename: Ton
authors.initials: T
authors.lastname: Bisseling
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Brewin
mesh_terms: D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D029902:Medicago / Q000201:enzymology* / Q000235:genetics*; D018519:Meristem / Q000201:enzymology; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics*; D018515:Plant Leaves / Q000201:enzymology; D018517:Plant Roots / Q000201:enzymology; D018520:Plant Shoots / Q000201:enzymology; D018547:Plant Stems / Q000201:enzymology; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping
chemical_list: D012333:RNA, Messenger; D019869:Phosphatidylinositol 3-Kinases