Tau isoforms which contain the domain encoded by exon 6 and their role in neurite elongation.
The regulation of tau protein expression during different stages of cellular differentiation and development as well as its functional role in morphogenesis, neurofibrillary tangle formation, and neurodegeneration have been topics of extensive study but have not been completely clarified yet. Tau undergoes complex regulated splicing in the mammalian nervous system. Our previous study with tau exon 6 demonstrated that it shows a splicing regulation profile which is distinct from that of the other tau exons as well as a unique expression pattern which is spatially and temporally regulated. In this study, we investigated the expression, localization, and effects of tau isoforms which contain exon 6 in neuroblastoma cells which stably overexpress them. We found that expression of one particular combination of tau exons (the longest adult isoform plus the domain of exon 6) significantly inhibits neurite elongation.
Luo
Min-Hua
MH
Neurobiology of Developmental Disorders, Shriver Center for Mental Retardation, Waltham, Massachusetts 02452, USA.
Leski
Michael L
ML
Andreadis
Athena
A
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D015153:Blotting, Western; D001923:Brain Chemistry; D019556:COS Cells; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D005091:Exons / Q000235:genetics*; D005333:Fetus / Q000737:chemistry; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D008099:Liver / Q000737:chemistry; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000737:chemistry; D016501:Neurites / Q000737:chemistry / Q000502:physiology*; D009474:Neurons / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D009842:Oligopeptides; D010455:Peptides / Q000235:genetics; D020033:Protein Isoforms / Q000032:analysis / Q000235:genetics / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D013116:Spinal Cord / Q000737:chemistry; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology; D014404:Tubulin / Q000032:analysis / Q000276:immunology; D016875:tau Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*
D000906:Antibodies; D009842:Oligopeptides; D010455:Peptides; D020033:Protein Isoforms; D014404:Tubulin; D016875:tau Proteins; D014212:Tretinoin; C085706:FLAG peptide