title: Evaluation of monocot and eudicot divergence using the sugarcane transcriptome.
abstract: Over 40,000 sugarcane (Saccharum officinarum) consensus sequences assembled from 237,954 expressed sequence tags were compared with the protein and DNA sequences from other angiosperms, including the genomes of Arabidopsis and rice (Oryza sativa). Approximately two-thirds of the sugarcane transcriptome have similar sequences in Arabidopsis. These sequences may represent a core set of proteins or protein domains that are conserved among monocots and eudicots and probably encode for essential angiosperm functions. The remaining sequences represent putative monocot-specific genetic material, one-half of which were found only in sugarcane. These monocot-specific cDNAs represent either novelties or, in many cases, fast-evolving sequences that diverged substantially from their eudicot homologs. The wide comparative genome analysis presented here provides information on the evolutionary changes that underlie the divergence of monocots and eudicots. Our comparative analysis also led to the identification of several not yet annotated putative genes and possible gene loss events in Arabidopsis.
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética, Universidade de Campinas, Caixa Postal 6010, 13083-970, Campinas SP, Brazil.
authors.forename: Michel
authors.initials: M
authors.lastname: Vincentz
authors.forename: Frank A A
authors.initials: FA
authors.lastname: Cara
authors.forename: Vagner K
authors.initials: VK
authors.lastname: Okura
authors.forename: Felipe R
authors.initials: FR
authors.lastname: da Silva
authors.forename: Guilherme L
authors.initials: GL
authors.lastname: Pedrosa
authors.forename: Adriana S
authors.initials: AS
authors.lastname: Hemerly
authors.forename: Adriana N
authors.initials: AN
authors.lastname: Capella
authors.forename: Mozart
authors.initials: M
authors.lastname: Marins
authors.forename: Paulo C
authors.initials: PC
authors.lastname: Ferreira
authors.forename: Suzelei C
authors.initials: SC
authors.lastname: França
authors.forename: Laurent
authors.initials: L
authors.lastname: Grivet
authors.forename: Andre L
authors.initials: AL
authors.lastname: Vettore
authors.forename: Edson L
authors.initials: EL
authors.lastname: Kemper
authors.forename: Willian L
authors.initials: WL
authors.lastname: Burnquist
authors.forename: Maria L P
authors.initials: ML
authors.lastname: Targon
authors.forename: Walter J
authors.initials: WJ
authors.lastname: Siqueira
authors.forename: Eiko E
authors.initials: EE
authors.lastname: Kuramae
authors.forename: Celso L
authors.initials: CL
authors.lastname: Marino
authors.forename: Luis E A
authors.initials: LE
authors.lastname: Camargo
authors.forename: Helaine
authors.initials: H
authors.lastname: Carrer
authors.forename: Luis L
authors.initials: LL
authors.lastname: Coutinho
authors.forename: Luiz R
authors.initials: LR
authors.lastname: Furlan
authors.forename: Manoel V F
authors.initials: MV
authors.lastname: Lemos
authors.forename: Luiz R
authors.initials: LR
authors.lastname: Nunes
authors.forename: Suely L
authors.initials: SL
authors.lastname: Gomes
authors.forename: Roberto V
authors.initials: RV
authors.lastname: Santelli
authors.forename: Maria H
authors.initials: MH
authors.lastname: Goldman
authors.forename: Maurício
authors.initials: M
authors.lastname: Bacci
authors.forename: Eder A
authors.initials: EA
authors.lastname: Giglioti
authors.forename: Otávio H
authors.initials: OH
authors.lastname: Thiemann
authors.forename: Flávio H
authors.initials: FH
authors.lastname: Silva
authors.forename: Marie-Anne
authors.initials: MA
authors.lastname: Van Sluys
authors.forename: Francisco G
authors.initials: FG
authors.lastname: Nobrega
authors.forename: Paulo
authors.initials: P
authors.lastname: Arruda
authors.forename: Carlos F M
authors.initials: CF
authors.lastname: Menck
mesh_terms: D017360:Arabidopsis / Q000145:classification / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics; D016384:Consensus Sequence; D019143:Evolution, Molecular; D020224:Expressed Sequence Tags; D018745:Genome, Plant; D019684:Magnoliopsida / Q000145:classification* / Q000235:genetics*; D012275:Oryza / Q000145:classification / Q000235:genetics; D031786:Saccharum / Q000145:classification* / Q000235:genetics*; D014158:Transcription, Genetic