Multiple formaldehyde oxidation/detoxification pathways in Burkholderia fungorum LB400.
Burkholderia species are free-living bacteria with a versatile metabolic lifestyle. The genome of B. fungorum LB400 is predicted to encode three different pathways for formaldehyde oxidation: an NAD-linked, glutathione (GSH)-independent formaldehyde dehydrogenase; an NAD-linked, GSH-dependent formaldehyde oxidation system; and a tetrahydromethanopterin-methanofuran-dependent formaldehyde oxidation system. The other Burkholderia species for which genome sequences are available, B. mallei, B. pseudomallei, and B. cepacia, are predicted to contain only the first two of these pathways. The roles of the three putative formaldehyde oxidation pathways in B. fungorum LB400 have been assessed via knockout mutations in each of these pathways, as well as in all combinations of knockouts. The resulting mutants have the expected loss of enzyme activities and exhibit defects of varying degrees of severity during growth on choline, a formaldehyde-producing substrate. Our data suggest that all three pathways are involved in formaldehyde detoxification and are functionally redundant under the tested conditions.
Marx
Christopher J
CJ
Department of Microbiology, University of Washington, Seattle, Washington 98195-2180, USA.
Miller
Jonathan A
JA
Chistoserdova
Ludmila
L
Lidstrom
Mary E
ME
D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000378:metabolism; D019117:Burkholderia / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D005557:Formaldehyde / Q000378:metabolism*; D009154:Mutation; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000235:genetics / Q000378:metabolism
D009243:NAD; D005557:Formaldehyde; C020145:formaldehyde dehydrogenase (glutathione); D000445:Aldehyde Oxidoreductases; C087006:formaldehyde dehydrogenase, glutathione-independent; D000587:Oxidoreductases Acting on CH-NH Group Donors; C047126:methylenetetrahydromethanopterin dehydrogenase