3D reconstruction of the hemocyanin subunit dimer from the chiton Acanthochiton fascicularis.
Procedures are presented for the purification of the subunit dimer from Acanthochiton fasicularis hemocyanin. Electron microscopy of negatively stained specimens revealed a uniform population of macromolecules possessing the characteristic "boat shape". A 3D reconstruction from this EM data generated a approximately 3 nm resolution model that correlates well with earlier data of the purported subunit dimer, extracted from the 3D reconstruction of the didecamer of Haliotis tuberculata hemocyanin type 1.
Harris
J Robin
JR
Institute of Zoology, Johannes Gutenberg University, D-55099, Mainz, Germany.
Meissner
Ulrich
U
Gebauer
Wolfgang
W
Markl
Jürgen
J
D000818:Animals; D019281:Dimerization; D006433:Hemocyanins / Q000737:chemistry*; D008854:Microscopy, Electron; D008974:Mollusca / Q000737:chemistry*; D021122:Protein Subunits
D021122:Protein Subunits; D006433:Hemocyanins