Determinants of platelet responsiveness to nitric oxide in patients with chronic heart failure.
Congestive heart failure (CHF) is associated with oxidative stress. Platelet responsiveness to nitric oxide (NO) donors, are impaired in patients with angina pectoris, possibly by increasing oxidative stress. We investigated the occurrence of platelet resistance to NO in patients, with ischaemic or non-ischaemic cardiomyopathy compared with normal subjects. Anti-aggregatory effects of sodium nitroprusside (SNP), oxidative stress and whole blood superoxide anion content were determined, with correlates of responsiveness to SNP. Inhibition of platelet aggregation by SNP was 65.4+/-3.55% in controls and 59.3+/-4.1% in CHF (P=ns) despite increased oxidative stress and post-aggregation O2- in CHF patients. However, subsets of CHF patients have NO-resistant platelets: this is associated with increasing age and/or increased oxidative stress (both p<0.05).
Anderson
R A
RA
Wales Heart Research Institute, University of Wales College of Medicine, Cardiff, Wales, UK. raanderson@ukgateway.net
Ellis
G R
GR
Chirkov
Y Y
YY
Holmes
A S
AS
Payne
N
N
Blackman
D J
DJ
Jackson
S K
SK
Lewis
M J
MJ
Horowitz
J D
JD
Frenneaux
M P
MP
D000328:Adult; D000367:Age Factors; D000368:Aged; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology / Q000627:therapeutic use; D016022:Case-Control Studies; D018929:Cell Culture Techniques; D005260:Female; D006333:Heart Failure / Q000188:drug therapy / Q000503:physiopathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology*; D018384:Oxidative Stress / Q000502:physiology*; D010974:Platelet Aggregation / Q000187:drug effects*; D012720:Severity of Illness Index
D000806:Angiotensin-Converting Enzyme Inhibitors; D020030:Nitric Oxide Donors; D009599:Nitroprusside