title: Expression and functional characterization of the system L amino acid transporter in KB human oral epidermoid carcinoma cells.
abstract: We have examined the expression and function of system l amino acid transporter in KB human oral epidermoid carcinoma cells. The KB cells express l-type amino acid transporter 1 (LAT1) in plasma membrane, but not l-type amino acid transporter 2 (LAT2). The [14C]l-leucine uptake by KB cells is inhibited by system l selective inhibitor BCH. The majority of [14C]l-leucine uptake is, therefore, mediated by LAT1. These results suggest that the transport of neutral amino acids including several essential amino acids into the KB cells mediated by LAT1 and the specific inhibition of LAT1 in oral cancer cells will be a new rationale for anti-cancer therapy.
authors.affiliation: Oral Biology Research Institute, Chosun University College of Dentistry, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, South Korea.
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Yoon
authors.forename: Youn Bae
authors.initials: YB
authors.lastname: Kim
authors.forename: Myong Soo
authors.initials: MS
authors.lastname: Kim
authors.forename: Joo Cheol
authors.initials: JC
authors.lastname: Park
authors.forename: Joong Ki
authors.initials: JK
authors.lastname: Kook
authors.forename: Hae Man
authors.initials: HM
authors.lastname: Jung
authors.forename: Saeng Gon
authors.initials: SG
authors.lastname: Kim
authors.forename: Hoon
authors.initials: H
authors.lastname: Yoo
authors.forename: Yeong Mu
authors.initials: YM
authors.lastname: Ko
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Bong Young
authors.initials: BY
authors.lastname: Kim
authors.forename: Hong Sung
authors.initials: HS
authors.lastname: Chun
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Kanai
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Endou
authors.forename: Do Kyung
authors.initials: DK
authors.lastname: Kim
mesh_terms: D027182:Amino Acid Transport System y+; D000598:Amino Acids, Cyclic / Q000494:pharmacology; D027301:Fusion Regulatory Protein 1, Light Chains / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007624:KB Cells / Q000737:chemistry*; D027283:Large Neutral Amino Acid-Transporter 1 / Q000032:analysis* / Q000502:physiology; D007930:Leucine / Q000378:metabolism
chemical_list: D027182:Amino Acid Transport System y+; D000598:Amino Acids, Cyclic; D027301:Fusion Regulatory Protein 1, Light Chains; D027283:Large Neutral Amino Acid-Transporter 1; C420646:SLC7A8 protein, human; C006504:2-aminobicyclo(2,2,1)heptane-2-carboxylic acid; D007930:Leucine