Quantitative autoradiography of dopamine receptors in the brains of micro-opioid receptor knockout mice.
Given the existence of functional interactions between opioidergic and dopaminergic systems, we have analyzed by quantitative autoradiography the possible long-term adaptive changes in the expression of D(1)- and D(2)-like dopamine receptors in the brains of mice lacking the micro-opioid receptor gene. An overall significant increase in D(1) and D(2) receptors (7.4 and 12.6%, respectively) across all cerebral regions examined was obtained in mutant mice relative to wild-type mice. However, region by region comparisons failed to reach significance in any individual brain area. These results indicate that only moderate changes in D(1)- and D(2)-like dopamine receptors densities occur in the brains of micro-opioid receptor knockout mice.
Léna
Isabelle
I
Pharmacology Group, School of Biomedical and Molecular Sciences, University of Surrey, Guildford, Surrey GU2 7XH, UK.
Matthes
Hans
H
Kieffer
Brigitte
B
Kitchen
Ian
I
D000704:Analysis of Variance; D000818:Animals; D001345:Autoradiography / Q000379:methods; D001552:Benzazepines / Q000493:pharmacokinetics; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D018492:Dopamine Antagonists / Q000493:pharmacokinetics; D051379:Mice; D018345:Mice, Knockout; D020891:Raclopride / Q000493:pharmacokinetics; D011954:Receptors, Dopamine / Q000032:analysis* / Q000145:classification; D017450:Receptors, Opioid, mu / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D014316:Tritium / Q000493:pharmacokinetics
D001552:Benzazepines; D018492:Dopamine Antagonists; D011954:Receptors, Dopamine; D017450:Receptors, Opioid, mu; D014316:Tritium; D020891:Raclopride