Determination of Acaulospora longula and Glomus subgroup Aa in plant roots from grassland using new primers against the large subunit ribosomal DNA.
Molecular techniques have become increasingly important for the identification of arbuscular mycorrhizal fungi (AMF). In this work Acaulospora longula and Glomus mosseae have been detected in plant roots from pastures using specific nucleotide primers for the two species. Part of the 5' end of the large subunit of the ribosomal RNA gene was amplified by nested PCR and sequenced. The distribution of the fungi within three different plant species, Plantago lanceolata, Trifolium repens, and Holcus lanatus, and two different types of grassland, have been studied. Neither the fungi nor the plants showed specific preference for their symbiotic partnership.
Geue
Holger
H
Technische Universitaet Muenchen, Center of Life Sciences Weihenstephan, Alte Akademie 12, D-85350 Freising, Germany. geue@wzw.tum.de
Hock
Bertold
B
D001483:Base Sequence; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D008969:Molecular Sequence Data; D038821:Mycorrhizae / Q000145:classification* / Q000235:genetics; D018517:Plant Roots / Q000382:microbiology*; D006109:Poaceae / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment; D013559:Symbiosis
D017931:DNA Primers; D004271:DNA, Fungal; D004275:DNA, Ribosomal