title: Regulation of endometrial stromal cell matrix metalloproteinase activity and invasiveness by interleukin-8.
abstract: OBJECTIVE
  To evaluate the effect of interleukin-8 (IL-8) on endometrial stromal cell metalloproteinase (MMP) activity and invasiveness.
  
  
  METHODS
  Experimental laboratory study.
  
  
  METHODS
  University medical center.
  
  
  METHODS
  Reproductive age women without endometriosis.
  
  
  METHODS
  Endometrial stromal cells were grown in culture and treated with recombinant IL-8 (0.0001-10 ng/mL) for 24 to 48 hours. Supernatants were collected for soluble assay of collagenase activity and gelatin zymography. Endometrial stromal cells were plated on 8-microm pore membranes coated with Matrigel or human simple matrix and treated with IL-8 for 48 hours.
  
  
  METHODS
  Collagenase activity and MMP2 and 9 activity of control vs. IL-8 treated endometrial cells, and number of endometrial cells that invade through Matrigel or human simple matrix.
  
  
  RESULTS
  Collagenase activity in cells treated with IL-8 (1-10 ng/mL) was higher than in control cells. On gelatin zymograms, MMP2 and MMP9 activity of endometrial stromal cells was stimulated by IL-8 treatment (1-10 ng/mL). The number of cells that invaded the Matrigel or human simple matrix was 1.7-fold higher in the group treated with 10 ng/mL of IL-8 compared with the control group.
  
  
  CONCLUSIONS
  IL-8 increases MMP activity and invasive capability of endometrial stromal cells in culture; IL-8 may be involved in the pathogenesis of endometriosis and menstrual physiology.
authors.affiliation: Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520-8063, USA.
authors.forename: Naciye
authors.initials: N
authors.lastname: Mulayim
authors.forename: Asim
authors.initials: A
authors.lastname: Savlu
authors.forename: Ozlem
authors.initials: O
authors.lastname: Guzeloglu-Kayisli
authors.forename: Umit A
authors.initials: UA
authors.lastname: Kayisli
authors.forename: Aydin
authors.initials: A
authors.lastname: Arici
mesh_terms: D000328:Adult; D001672:Biocompatible Materials; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen; D017364:Collagenases / Q000737:chemistry / Q000378:metabolism; D004338:Drug Combinations; D004717:Endometrium / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology / Q000502:physiology*; D007797:Laminin; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D011509:Proteoglycans; D012995:Solubility; D017154:Stromal Cells / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D004338:Drug Combinations; D016209:Interleukin-8; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen; D017364:Collagenases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9