Antioxidants and inhibitor of matrix metalloproteinase-1 expression from leaves of Zostera marina L.
In order to develop new anti-photoaging agents, we examined the antioxidative activity and the inhibition effect of matrix metalloproteinase-1 (MMP-1) on the extracts of a marine product, Zostera marina L., which is known for its potent activity. Three compounds (compounds 1, 2, and 3) were isolated from an ethyl acetate (EtOAc) soluble fraction of the product; they were identified as apigenin-7-O-beta-D-glucoside (1), chrysoeriol (2), and luteolin (3). These compounds were found to scavenge radicals and reactive oxygen species (ROS) and were measured to have SC50 values of 0.18 mM, 0.68 mM, and 0.01 mM against the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 0.04 mM, 0.03 mM, and 0.01 mM against the superoxide radical in the xanthine/xanthine oxidase system, respectively. Compound 3 suppressed the expression of MMP-1 by up to 44% at 4.0 microM and inhibited the production of interleukin 6 (IL-6), which is known as a cytokine that induces MMP-1 expression. From these results, compound 3 and the other compounds were determined to have antioxidative activity and to inhibit MMP-1 expression. Thus, the three compounds are expected to be useful for preventing the photoaging of skin.
Kim
Jin Hui
JH
R&D Center, Hanbul Cosmetic Corporation, 72-7 Yongsung-ri, Samsung-Myun, Umsung-Kun, Chungbuk 369-830, Korea. jiny@hanbul.co.kr
Cho
Young Ho
YH
Park
Sung Min
SM
Lee
Kyung Eun
KE
Lee
Jeong Jae
JJ
Lee
Bum Chun
BC
Pyo
Hyeong Bae
HB
Song
Kyung Sik
KS
Park
Hum Dai
HD
Yun
Yeo Pyo
YP
D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D001713:Biphenyl Compounds; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D061965:Matrix Metalloproteinase Inhibitors; D010851:Picrates / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D011480:Protease Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology*; D013481:Superoxides / Q000737:chemistry; D013778:Tetrazolium Salts; D013844:Thiazoles; D014466:Ultraviolet Rays; D035962:Zosteraceae / Q000737:chemistry*
D000975:Antioxidants; D001713:Biphenyl Compounds; D016207:Cytokines; D061965:Matrix Metalloproteinase Inhibitors; D010851:Picrates; D011480:Protease Inhibitors; D013778:Tetrazolium Salts; D013844:Thiazoles; D013481:Superoxides; C004931:1,1-diphenyl-2-picrylhydrazyl; C022616:thiazolyl blue