One-hybrid screens at the Saccharomyces cerevisiae HMR locus identify novel transcriptional silencing factors.
In Saccharomyces cerevisiae, genes located at the telomeres and the HM loci are subject to transcriptional silencing. Here, we report results of screening a Gal4 DNA-binding domain hybrid library for proteins that cause silencing when targeted to a silencer-defective HMR locus.
Andrulis
Erik D
ED
Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York 11794, USA.
Zappulla
David C
DC
Alexieva-Botcheva
Krassimira
K
Evangelista
Carlos
C
Sternglanz
Rolf
R
D004268:DNA-Binding Proteins; D020868:Gene Silencing; D005800:Genes, Fungal; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae / Q000235:genetics; D016615:Telomere / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D020798:Two-Hybrid System Techniques
D004268:DNA-Binding Proteins; C052130:GAL4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae; D014157:Transcription Factors