Active-site dynamics in RNA polymerases.
New crystal structures of transcription complexes formed by bacteriophage T7 RNA polymerase reveal a nucleotide-addition cycle driven by active-site conformational changes similar to those observed in DNA polymerases, and suggest provocative hypotheses for the more complex multisubunit RNA polymerases of free-living organisms.
Landick
Robert
R
Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.
D000818:Animals; D001665:Binding Sites / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics*; D015967:Gene Expression Regulation, Viral / Q000235:genetics*; D006801:Humans; D008968:Molecular Conformation; D009711:Nucleotides / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis*; D014178:Translocation, Genetic / Q000235:genetics; D014764:Viral Proteins
D009711:Nucleotides; D012333:RNA, Messenger; D014764:Viral Proteins; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases; D004259:DNA-Directed DNA Polymerase