Immunomodulating effect of antimicrobial agents on cytokine production by human polymorphonuclear neutrophils.
It has been previously demonstrated that some antimicrobial agents enhance activities of human polymorphonuclear neutrophils (PMNs). The effect on the release of cytokines in an inflammatory context from PMNs by these antibiotics was evaluated. We studied the effect of the release of some cytokines by human PMNs RT-PCR analysis on a clinical strain of Klebsiella pneumoniae by comparing the effect with that observed in the presence of co-amoxiclav, sanfetrinem, clarithromycin, prulifloxacin and tobramycin. All the drugs tested were capable of modulating PMN synthesis in vitro of pro-inflammatory cytokines IL-8, IL-1beta, TNF-alpha and IL-6, but not that of anti-inflammatory cytokine IL-10. The degree of their stimulatory or inhibitory potency varied with the cytokine examined.
Reato
Gigliola
G
Department of Biomedical Sciences and Human Oncology, University of Turin, Turin, Italy.
Cuffini
Anna Maria
AM
Tullio
Vivian
V
Mandras
Narcisa
N
Roana
Janira
J
Banche
Giuliana
G
Foa
Robin
R
Carlone
Nicola A
NA
D000900:Anti-Bacterial Agents / Q000276:immunology* / Q000494:pharmacology; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007249:Inflammation / Q000276:immunology; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D018375:Neutrophil Activation / Q000187:drug effects; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism*; D016133:Polymerase Chain Reaction
D000900:Anti-Bacterial Agents; D016207:Cytokines; D008070:Lipopolysaccharides