title: Generation of protein in situ arrays by DiscernArray technology.
abstract: A cell-free DiscernArray technology is described for making protein arrays directly from polymerase chain reaction deoxyribonucleic acid (PCR DNA) fragments. In this method, individual tagged proteins are synthesized in a cell-free system on a tag-binding surface, such that the tagged proteins are immobilized on the surface as they are produced. This technology is particularly useful for arraying proteins/domains that cannot be functionally produced by heterologous expression or for which the cloned DNA is not available. Protein arrays of single-chain antibodies, ligand-binding domains, and enzymes have been successfully generated by this method.
authors.affiliation: Discerna Limited, Cambridge, UK.
authors.forename: Mingyue
authors.initials: M
authors.lastname: He
mesh_terms: D002474:Cell-Free System; D016133:Polymerase Chain Reaction / Q000379:methods; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D011506:Proteins