title: Liquid chromatography method for determination of bivalirudin in human plasma and urine using automated ortho-phthalaldehyde derivatization and fluorescence detection.
abstract: A high-performance liquid chromatographic (HPLC) method was developed using solid-phase extraction, o-phthalaldehyde (OPA) derivatization and fluorescence detection for the determination of the direct thrombin inhibitor bivalirudin in human plasma and urine. The use of this assay will facilitate the study of the pharmacodynamics of bivalirudin in studies of special patient populations. A C(18) bioanalytical column at a flow rate of 1 ml/min with an aqueous trifluoroacetic acid (0.1% TFA in deionized water, pH 2.2, v/v) mobile phase and methanol gradient was used. The assay demonstrated linearity from 3 to 20 microg/ml bivalirudin in plasma, with a detection limit of 1 microg/ml. The method was utilized in a study evaluating the pharmacokinetic and pharmacodynamic effects of bivalirudin in patients undergoing percutaneous coronary interventions (PCIs).
authors.affiliation: Division of Nephrology, Clinical Pharmacology and Hypertension, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298-0160, USA. don.e.farthing@pmusa.com
authors.forename: Don
authors.initials: D
authors.lastname: Farthing
authors.forename: Terri
authors.initials: T
authors.lastname: Larus
authors.forename: Itaf
authors.initials: I
authors.lastname: Fakhry
authors.forename: Todd
authors.initials: T
authors.lastname: Gehr
authors.forename: Jayne
authors.initials: J
authors.lastname: Prats
authors.forename: Domenic
authors.initials: D
authors.lastname: Sica
mesh_terms: D001331:Automation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006629:Hirudins / Q000031:analogs & derivatives* / Q000097:blood* / Q000652:urine*; D006801:Humans; D010446:Peptide Fragments / Q000097:blood* / Q000652:urine*; D011994:Recombinant Proteins / Q000097:blood* / Q000652:urine*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D009764:o-Phthalaldehyde / Q000737:chemistry*
chemical_list: D006629:Hirudins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D009764:o-Phthalaldehyde; C074619:bivalirudin