title: Processing of photoaptamer microarrays.
abstract: Photoaptamers are single-stranded nucleic acids selected for their high affinity to specific proteins of interest. Photoaptamer microarrays capture and quantify proteins from complex samples using a unique protocol that leverages both high-affinity capture with covalent retention of analytes. The initial capture of proteins from solution is similar to the well-known antibody capture, but the "secondary binding event" affected by photoaptamers is a covalent crosslink between the photoaptamer capture agent and the protein analyte. The nature of this specific covalent reaction allows a unique microarray processing that is described in detail in this chapter.
authors.affiliation: SomaLogic Inc, Boulder, CO, USA.
authors.forename: Helen
authors.initials: H
authors.lastname: Petach
authors.forename: Rachel
authors.initials: R
authors.lastname: Ostroff
authors.forename: Chad
authors.initials: C
authors.lastname: Greef
authors.forename: Gregory M
authors.initials: GM
authors.lastname: Husar
mesh_terms: D003432:Cross-Linking Reagents / Q000378:metabolism; D043169:Endostatins / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D008027:Light; D009696:Nucleic Acids / Q000378:metabolism*; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011485:Protein Binding
chemical_list: D003432:Cross-Linking Reagents; D043169:Endostatins; D005456:Fluorescent Dyes; D009696:Nucleic Acids