The construction of endothelial cellular biosensing system for the control of blood pressure drugs.
In order to assess blood pressure control drugs, the endothelial cellular biosensing system for assessing blood pressure control drugs was constructed. This system consists of human umbilical vein endothelial cells (HUVEC) on a polyion-coated gold electrode, a platinum counter electrode and an Ag/AgCl reference electrode. Nitric oxide (NO) as an indicator of blood vessel relaxation was detected with a polyion-coated electrode in the system. The NO detection limit of this electrode was 8.4 nM by differential pulse voltammetry (DPV). The drugs of blood pressure control (acetylcholine chloride (AcChCl), NOC 7 and NG-monomethyl-L-arginine (L-NMMA)) were assessed with this endothelial cellular biosensing system. One milli molar of AcChCl make NO released from HUVEC stimulated by activating endothelial nitric oxide synthase (eNOS) in HUVEC. In the case of 5 mM of L-NMMA, NO releasing was inhibited by inhibiting eNOS activation by 1 mM of AcChCl. NOC 7 immediately released NO regardless of eNOS activation in endothelial cells.
Kamei
Ken-ichiro
K
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Haruyama
Tetsuya
T
Mie
Masayasu
M
Yanagida
Yasuko
Y
Aizawa
Masuo
M
Kobatake
Eiry
E
D000959:Antihypertensive Agents / Q000378:metabolism*; D015374:Biosensing Techniques; D042783:Endothelial Cells / Q000378:metabolism; D008564:Membrane Potentials; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D013997:Time Factors
D000959:Antihypertensive Agents; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase