title: Pasteurella multocida contains multiple immunogenic haemin- and haemoglobin-binding proteins.
abstract: Iron-dependent outer membrane proteins (IROMPs) play an important role in bacterial pathogenesis and present several attributes of potential vaccine candidates. TBLASTN analysis of the Pasteurella multocida Pm70 genome using the same molecules of other bacterial pathogens as a query identified eight putative haemin and haemoglobin receptors for this organism. Quantitative binding assays have demonstrated that the proteins PM0040, PM0236, PM0741, PM1081, PM1428, PM0592 and HgbA bind both haemin and haemoglobin, whereas PM0576 and PM1282 ORFs only bind either haemoglobin or haemin, respectively. Furthermore, Western blot analysis showed that P. multocida-infected mice generate specific antibodies against PM0040, PM0236, PM0741, PM1081, PM1428, PM0592 and HgbA proteins. Nevertheless, inoculation of mice with any single one of these receptors alone did not protect against P. multocida infection.
authors.affiliation: Department of Genetics and Microbiology, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Bosch
authors.forename: M Elena
authors.initials: ME
authors.lastname: Garrido
authors.forename: Ana M
authors.initials: AM
authors.lastname: Pérez de Rozas
authors.forename: Ignacio
authors.initials: I
authors.lastname: Badiola
authors.forename: Jordi
authors.initials: J
authors.lastname: Barbé
authors.forename: Montserrat
authors.initials: M
authors.lastname: Llagostera
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism*; D005260:Female; D016680:Genome, Bacterial; D000081062:Heme-Binding Proteins; D006420:Hemeproteins / Q000276:immunology / Q000378:metabolism*; D006427:Hemin / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D015151:Immunoblotting; D051379:Mice; D010326:Pasteurella Infections / Q000276:immunology* / Q000378:metabolism; D016979:Pasteurella multocida / Q000276:immunology / Q000378:metabolism*
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D000081062:Heme-Binding Proteins; D006420:Hemeproteins; D006454:Hemoglobins; C017206:hemoglobin-binding protein, bacteria; D006427:Hemin