Enhanced long-term survival, but no increase in replicative capacity, following retroviral transduction of human cord blood CD34+ cells with human telomerase reverse transcriptase.
Overexpression of telomerase reverse transcriptase (hTERT) can immortalize some primary human mesenchymal cells. We investigated whether retrovirally-mediated expression of hTERT in CD34+ umbilical cord blood (UCB) cells can extend the replicative lifespan of human hematopoietic progenitor cells. Overexpression of hTERT did not immortalize these cells but did lead to enhanced survival of mature hematopoietic cells.
Elwood
Ngaire J
NJ
Jiang
Xu-Rong
XR
Chiu
Choy-Pik
CP
Lebkowski
Jane S
JS
Smith
Clayton A
CA
D018952:Antigens, CD34 / Q000097:blood*; D002455:Cell Division; D002470:Cell Survival; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005312:Fetal Blood / Q000166:cytology* / Q000201:enzymology* / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000201:enzymology; D006801:Humans; D012190:Retroviridae / Q000502:physiology; D019098:Telomerase / Q000378:metabolism*; D014161:Transduction, Genetic
D018952:Antigens, CD34; D004268:DNA-Binding Proteins; D019098:Telomerase