title: Semi-automated 86Y purification using a three-column system.
abstract: The separation of (86)Y from (86)Sr was optimized by a semi-automated purification system involving the passage of the target sample through three sequential columns. The target material was dissolved in 4 N HNO(3) and loaded onto a Sr-selective (Sr-Spec) column to retain the (86)Sr. The yttrium was eluted with 4 N HNO(3) onto the second Y-selective (RE-Spec) column with quantitative retention. The RE-Spec column was eluted with a stepwise decreasing concentration of HNO(3) to wash out potential metallic impurities to a waste container. The eluate was then pumped onto an Aminex A5 column with 0.1 N HCl and finally with 3 N HCl to collect the radioyttrium in 0.6-0.8 mL with a >80% recovery. This method enabled us to decontaminate Sr by 250,000 times and label 30 micro g of DOTA-Biotin with a >95% yield.
authors.affiliation: Nuclear Medicine Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Luke S
authors.initials: LS
authors.lastname: Park
authors.forename: Lawrence P
authors.initials: LP
authors.lastname: Szajek
authors.forename: Karen J
authors.initials: KJ
authors.lastname: Wong
authors.forename: Paul S
authors.initials: PS
authors.lastname: Plascjak
authors.forename: Kayhan
authors.initials: K
authors.lastname: Garmestani
authors.forename: Shawn
authors.initials: S
authors.lastname: Googins
authors.forename: William C
authors.initials: WC
authors.lastname: Eckelman
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Carrasquillo
authors.forename: Chang H
authors.initials: CH
authors.lastname: Paik
mesh_terms: D001710:Biotin / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry*; D002845:Chromatography / Q000295:instrumentation* / Q000379:methods*; D007553:Isotope Labeling / Q000379:methods*; D009942:Organometallic Compounds / Q000032:analysis / Q000737:chemistry*; D011485:Protein Binding; D019275:Radiopharmaceuticals / Q000138:chemical synthesis; D012371:Robotics / Q000379:methods*; D013325:Strontium Isotopes / Q000302:isolation & purification*; D015021:Yttrium Radioisotopes / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: C405616:DOTA-biotin; D009942:Organometallic Compounds; D019275:Radiopharmaceuticals; D013325:Strontium Isotopes; D015021:Yttrium Radioisotopes; D001710:Biotin