title: Short consensus probes with 3'-minor groove binder of the immunoglobulin heavy-chain gene for real-time quantitative PCR in B-cell non-Hodgkin lymphomas.
abstract: We used 3'-minor groove binder (MGB) technology to develop consensus fluorogenically labeled probes of the immunoglobulin heavy-chain (IgH) gene for detecting minimal residual disease (MRD) in B-cell non-Hodgkin lymphoma (B-NHL). Sequence data from 59 patients with B-NHLs revealed a narrow consensus region as a result of somatic hypermutations and variable VH usage, indicating that it would be difficult to design ordinary non-MGB probes. MGB probes, characterized by shorter length but higher melting temperature, are more suitable for this situation than ordinary non-MGB probes. In fact, the present data indicated that about 20% more cases were detectable with MGB probes (34/59, 57.6%) than with the non-MGB probes (23/59, 39.0%) designed by Donovan et al. MGB technology is useful for the design of consensus fluorogenically labeled probes of the IgH gene for detecting MRD.
authors.affiliation: Department of Pathology, Iwate Medical University School of Medicine, Uchimaru 19-1, 020-8505 Morioka, Japan.
authors.forename: Michihiro
authors.initials: M
authors.lastname: Uchiyama
authors.forename: Chihaya
authors.initials: C
authors.lastname: Maesawa
authors.forename: Akiko
authors.initials: A
authors.lastname: Yashima-Abo
authors.forename: Mitsu
authors.initials: M
authors.lastname: Tarusawa
authors.forename: Mamoru
authors.initials: M
authors.lastname: Satoh
authors.forename: Takashi
authors.initials: T
authors.lastname: Satoh
authors.forename: Yoji
authors.initials: Y
authors.lastname: Ishida
authors.forename: Shigeki
authors.initials: S
authors.lastname: Ito
authors.forename: Kazunori
authors.initials: K
authors.lastname: Murai
authors.forename: Sanae
authors.initials: S
authors.lastname: Enomoto
authors.forename: Taiju
authors.initials: T
authors.lastname: Utsugisawa
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Masuda
mesh_terms: D001483:Base Sequence; D015342:DNA Probes; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D005803:Genes, Immunoglobulin; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D018365:Neoplasm, Residual; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D015342:DNA Probes; D007143:Immunoglobulin Heavy Chains