title: Real-time polymerase chain reaction for detecting SARS coronavirus, Beijing, 2003.
abstract: During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3' end of the viral genome, was established to detect and identify the SARS-associated coronavirus. We describe the use of this assay to screen >700 clinical samples.
authors.affiliation: Institute of Microbiology and Epidemiology, Academy of Military Sciences, Beijing, China.
authors.forename: Junhui
authors.initials: J
authors.lastname: Zhai
authors.forename: Thomas
authors.initials: T
authors.lastname: Briese
authors.forename: Erhei
authors.initials: E
authors.lastname: Dai
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: pang
authors.forename: Zongmin
authors.initials: Z
authors.lastname: Du
authors.forename: Haihong
authors.initials: H
authors.lastname: Liu
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Hongxia
authors.initials: H
authors.lastname: Wang
authors.forename: Zhaobiao
authors.initials: Z
authors.lastname: Guo
authors.forename: Zeliang
authors.initials: Z
authors.lastname: Chen
authors.forename: Lingxiao
authors.initials: L
authors.lastname: Jiang
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhou
authors.forename: Yanping
authors.initials: Y
authors.lastname: Han
authors.forename: Omar
authors.initials: O
authors.lastname: Jabado
authors.forename: Gustavo
authors.initials: G
authors.lastname: Palacios
authors.forename: W Ian
authors.initials: WI
authors.lastname: Lipkin
authors.forename: Ruifu
authors.initials: R
authors.lastname: Tang
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004196:Disease Outbreaks; D005243:Feces / Q000821:virology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D012367:RNA, Viral / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D045169:Severe Acute Respiratory Syndrome / Q000453:epidemiology / Q000276:immunology / Q000821:virology; D013997:Time Factors
chemical_list: D000914:Antibodies, Viral; D017931:DNA Primers; D004279:DNA, Viral; D012367:RNA, Viral