title: Arabidopsis RAV1 is down-regulated by brassinosteroid and may act as a negative regulator during plant development.
abstract: RAV1 is a novel DNA-binding protein with two distinct DNA-binding domains unique in higher plants, but its role in plant growth and development remains unknown. Using cDNA array, we found that transcription of RAV1 is down-regulated by epibrassinolide (epiBL) in Arabidopsis suspension cells. RNA gel blot analysis revealed that epiBL-regulated RAV1 transcription involves neither protein phosphorylation/dephosphorylation nor newly synthesized protein, and does not require the functional BRI1, suggesting that this regulation might be through a new BR signaling pathway. Overexpressing RAV1 in Arabidopsis results in a retardation of lateral root and rosette leaf development, and the underexpression causes an earlier flowering phenotype, implying that RAV1 may function as a negative regulatory component of growth and development.
authors.affiliation: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yu Xin
authors.initials: YX
authors.lastname: Hu
authors.forename: Yong Xong
authors.initials: YX
authors.lastname: Wang
authors.forename: Xin Fang
authors.initials: XF
authors.lastname: Liu
authors.forename: Jia Yang
authors.initials: JY
authors.lastname: Li
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D060406:Brassinosteroids; D002777:Cholestanols / Q000494:pharmacology*; D003513:Cycloheximide / Q000494:pharmacology; D016373:DNA, Antisense / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D015536:Down-Regulation / Q000187:drug effects; D035264:Flowers / Q000254:growth & development; D018628:Gene Dosage; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D019319:Okadaic Acid / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000254:growth & development; D018517:Plant Roots / Q000254:growth & development; D030821:Plants, Genetically Modified; D011494:Protein Kinases / Q000378:metabolism; D012231:Rhizobium / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects; D019311:Staurosporine / Q000494:pharmacology; D013260:Steroids, Heterocyclic / Q000494:pharmacology*; D014170:Transformation, Genetic / Q000235:genetics; D015026:Zeatin / Q000494:pharmacology
chemical_list: D029681:Arabidopsis Proteins; D060406:Brassinosteroids; D002777:Cholestanols; D016373:DNA, Antisense; D004268:DNA-Binding Proteins; D010937:Plant Growth Regulators; C117695:RAV1 protein, Arabidopsis; D013260:Steroids, Heterocyclic; D019319:Okadaic Acid; D015084:2,4-Dichlorophenoxyacetic Acid; D015026:Zeatin; D003513:Cycloheximide; D011494:Protein Kinases; C108270:BRI1 protein, Arabidopsis; D019311:Staurosporine; C023623:brassinolide