title: Chronic hypoperfusion alters the content and structure of proteins and lipids of rat brain homogenates: a Fourier transform infrared spectroscopy study.
abstract: Arteriovenous malformations (AVMs), masses of abnormal blood vessels which grow in the brain, produce high flow shunts that steal blood from surrounding brain tissue, which is chronically hypoperfused. Hypoperfusion is a condition of inadequate tissue perfusion and oxygenation, resulting in abnormal tissue metabolism. Fourier transform infrared (FTIR) spectroscopy is used in this study to investigate the effect of hypoperfusion on homogenized rat brain samples at the molecular level. The results suggest that the lipid content increases, the protein content decreases, the lipid-to-protein ratio increases, and the state of order of the lipids increases in the hypoperfused brain samples. FTIR results also revealed that, owing to hypoperfusion, not only the protein synthesis but also the protein secondary structure profile is altered in favor of beta-sheets and random coils. These findings clearly demonstrate that, FTIR spectroscopy can be used to extract valuable information at the molecular level so as to have a better understanding of the effect of hypoperfusion on rat brain.
authors.affiliation: Department of Biology, Middle East Technical University, 06531 Ankara, Turkey.
authors.forename: Neslihan
authors.initials: N
authors.lastname: Toyran
authors.forename: Faruk
authors.initials: F
authors.lastname: Zorlu
authors.forename: Gizem
authors.initials: G
authors.lastname: Dönmez
authors.forename: Kamil
authors.initials: K
authors.lastname: Oğe
authors.forename: Feride
authors.initials: F
authors.lastname: Severcan
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002545:Brain Ischemia / Q000378:metabolism*; D008055:Lipids / Q000032:analysis* / Q000737:chemistry*; D008297:Male; D008968:Molecular Conformation; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D014020:Tissue Extracts / Q000032:analysis / Q000737:chemistry
chemical_list: D008055:Lipids; D011506:Proteins; D014020:Tissue Extracts