Characterization of keyhole limpet hemocyanin (KLH) glycans sharing a carbohydrate epitope with Schistosoma mansoni glycoconjugates.
Keyhole limpet hemocyanin (KLH) is known to share carbohydrate epitopes with Schistosoma mansoni. In order to define the structural basis for the observed serological cross-reactivity, KLH glycans were released either by enzyme treatment or by hydrazinolysis and probed with a rabbit hyperimmune serum directed against S. mansoni egg antigen. Both major, non-reacting oligosaccharide species as well as the minor compounds recognized were isolated by two-dimensional high performance liquid chromatography and in part by lectin affinity chromatography, and characterized by mass spectrometry. The results revealed that KLH carries predominantly high mannose-type glycans as well as short sugar chains. As a characteristic feature, a number of the latter glycans contained a Gal(beta1-6)Man-unit, which has not yet been found in glycoprotein-N-glycans. Oligosaccharides cross-reacting with schistosomal glycans comprised a terminal Fuc(alpha1-3)GalNAc-motif, which appears to represent the main carbohydrate epitope mediating cross-reactivity of KLH with glycoconjugates from S. mansoni.
Geyer
Hildegard
H
Faculty of Medicine, Institute of Biochemistry, University of Giessen, Friedrichstrasse 24, Giessen D-35392, Germany.
Wuhrer
Manfred
M
Kurokawa
Tomofumi
T
Geyer
Rudolf
R
D000818:Animals; D000939:Epitopes; D006001:Glycoconjugates / Q000276:immunology*; D006433:Hemocyanins / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D012550:Schistosoma mansoni / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000939:Epitopes; D006001:Glycoconjugates; D011134:Polysaccharides; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin