High efficiency of transferring a native sugar chain from a glycopeptide by a microbial endoglycosidase in organic solvents.
We examined the transglycosylation reaction by the recombinant endo-beta-N-acetylglucosaminidase from Mucor hiemalis (Endo-M) expressed in Candida boidinii in media containing organic solvents. The recombinant Endo-M could transglycosylate a disialo biantennary complex-type oligosaccharide from hen egg yolk glycopeptide to p-nitrophenyl N-acetyl-beta-D-glucosaminide even in the presence of 30% acetone, dimethyl sulfoxide, or methanol. The yield of the transglycosylation product reached 21-34% of the total amount of acceptor, while the yield was only about 14% in aqueous solution.
Akaike
Eri
E
The Noguchi Institute, 1-8-1 Kaga, Itabashi-ku, Tokyo 173-0003, Japan.
Tsutsumida
Maki
M
Osumi
Kenji
K
Fujita
Masaya
M
Yamanoi
Takashi
T
Yamamoto
Kenji
K
Fujita
Kiyotaka
K
D002175:Candida / Q000201:enzymology*; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006020:Glycopeptides / Q000737:chemistry*; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase / Q000378:metabolism*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009930:Organic Chemicals / Q000737:chemistry; D012997:Solvents / Q000737:chemistry*
D002241:Carbohydrates; D006020:Glycopeptides; D009930:Organic Chemicals; D012997:Solvents; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase