title: Characterization of keyhole limpet hemocyanin (KLH) glycans sharing a carbohydrate epitope with Schistosoma mansoni glycoconjugates.
abstract: Keyhole limpet hemocyanin (KLH) is known to share carbohydrate epitopes with Schistosoma mansoni. In order to define the structural basis for the observed serological cross-reactivity, KLH glycans were released either by enzyme treatment or by hydrazinolysis and probed with a rabbit hyperimmune serum directed against S. mansoni egg antigen. Both major, non-reacting oligosaccharide species as well as the minor compounds recognized were isolated by two-dimensional high performance liquid chromatography and in part by lectin affinity chromatography, and characterized by mass spectrometry. The results revealed that KLH carries predominantly high mannose-type glycans as well as short sugar chains. As a characteristic feature, a number of the latter glycans contained a Gal(beta1-6)Man-unit, which has not yet been found in glycoprotein-N-glycans. Oligosaccharides cross-reacting with schistosomal glycans comprised a terminal Fuc(alpha1-3)GalNAc-motif, which appears to represent the main carbohydrate epitope mediating cross-reactivity of KLH with glycoconjugates from S. mansoni.
authors.affiliation: Faculty of Medicine, Institute of Biochemistry, University of Giessen, Friedrichstrasse 24, Giessen D-35392, Germany.
authors.forename: Hildegard
authors.initials: H
authors.lastname: Geyer
authors.forename: Manfred
authors.initials: M
authors.lastname: Wuhrer
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Kurokawa
authors.forename: Rudolf
authors.initials: R
authors.lastname: Geyer
mesh_terms: D000818:Animals; D000939:Epitopes; D006001:Glycoconjugates / Q000276:immunology*; D006433:Hemocyanins / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D012550:Schistosoma mansoni / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000939:Epitopes; D006001:Glycoconjugates; D011134:Polysaccharides; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin