Determination of 1-deoxynojirimycin in mulberry leaves using hydrophilic interaction chromatography with evaporative light scattering detection.
A simple and rapid method for determining 1-deoxynojirimycin (DNJ), a potent glucosidase inihibitor present in mulberry leaves (Morus alba and Morus bombysis), by high-performance liquid chromatography coupled to an evaporative light scattering detector (ELSD) has been developed. DNJ was separated from an extract of mulberry leaves on a TSKgel Amide-80 column, which is a representative column for hydrophilic interaction chromatography. During postcolumn detection, DNJ was detected by ELSD and concurrently identified by mass spectrometry. The detection limit was 100 ng. This method is sufficiently sensitive for determining DNJ in mulberry leaves and other related products.
Kimura
Toshiyuki
T
National Agricultural Research Center for Tohoku Region, Fukushima 960-2156, Japan. kmr@affrc.go.jp
Nakagawa
Kiyotaka
K
Saito
Yuko
Y
Yamagishi
Kenji
K
Suzuki
Masahiro
M
Yamaki
Kohji
K
Shinmoto
Hiroshi
H
Miyazawa
Teruo
T
D017485:1-Deoxynojirimycin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008027:Light; D031625:Morus / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D012542:Scattering, Radiation
D017485:1-Deoxynojirimycin