Determination of aucubin and catalpol in Plantago species by micellar electrokinetic chromatography.
Micellar electrokinetic chromatography (MEKC) was used for the separation and determination of two iridoid glycosides, aucubin and catalpol, in several Plantago species growing in Croatia: P. altissima L., P. argentea Chaix, P. coronopus L., P. holosteum Scop. (subsp. depauperata, subsp. holosteum and subsp. scopulorum), P. lagopus L., P. lanceolata L., and P. maritima L. Hot water extraction (HWE) was applied for the isolation of iridoid substances. Significant differences appeared between the iridoid contents in the examined species. The yield of aucubin and catalpol was up to 0.27% and 1.81% of the dry mass of the leaves, respectively. Besides aucubin and catalpol, two related compounds were determined in the plant samples.
Jurisić
Renata
R
Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovacića 1, HR-10000 Zagreb, Croatia. rjurisic@pharma.hr
Debeljak
Zeljko
Z
Vladimir-Knezević
Sanda
S
Vuković
Jadranka
J
D002845:Chromatography; D017523:Croatia; D004563:Electrochemistry; D019075:Electrophoresis, Capillary; D005960:Glucosides / Q000737:chemistry* / Q000302:isolation & purification; D057888:Iridoid Glucosides; D039823:Iridoids / Q000737:chemistry* / Q000302:isolation & purification; D008823:Micelles; D008958:Models, Molecular; D015394:Molecular Structure; D010943:Plantago / Q000737:chemistry* / Q000145:classification; D013045:Species Specificity; D013053:Spectrophotometry
D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D008823:Micelles; C078040:catalpol; C006650:aucubin