tRNA-intergenic spacer PCR for the identification of Pasteurella and Mannheimia spp.
tRNA-intergenic spacer PCR (tDNA-PCR) was evaluated for its effectiveness in differentiating Pasteurella and Mannheimia (sub)species predominantly of ruminant origin. For this purpose, 38 reference strains and 13 field isolates belonging to both genera were investigated. tDNA-PCR enabled discrimination of all Pasteurella species tested (Pasteurella (P.) aerogenes, P. avium, P. canis, P. lymphangitidis, P. multocida, P. trehalosi). For the differentiation of the subspecies of P. multocida, an additional dulcitol reaction was required. Two of the five so far-defined Mannheimia species, M. granulomatis and M. varigena, had a distinct fingerprinting profile. The remaining three phylogenetically highly related species (M. haemolytica, M. glucosida, and M. ruminalis) clustered together. Nevertheless, M. ruminalis is non-haemolytic, and M. haemolytica and M. glucosida can be differentiated on the basis of two additional phenotypic characteristics (beta-glucosidase and aesculin hydrolysis). In conclusion, tDNA-PCR is a useful tool in differentiating organisms belonging to the genera Pasteurella and Mannheimia.
Catry
Boudewijn
B
Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. boudewijn.catry@ugent.be
Baele
Margo
M
Opsomer
Geert
G
de Kruif
Aart
A
Decostere
Annemie
A
Haesebrouck
Freddy
F
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D021901:DNA, Intergenic / Q000737:chemistry / Q000235:genetics; D019075:Electrophoresis, Capillary / Q000662:veterinary; D006801:Humans; D031121:Mannheimia / Q000235:genetics* / Q000302:isolation & purification; D010325:Pasteurella / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000662:veterinary; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics*; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*
D004269:DNA, Bacterial; D021901:DNA, Intergenic; D012343:RNA, Transfer