title: 68Ga-labelled DOTA-derivatised peptide ligands.
abstract: 68Ge/68Ga generators provide cyclotron-independent access to positron emission tomography (PET) radiopharmaceuticals. We describe a system which allows the safe and efficient handling of 68Ge/68Ga generator eluates for labelling of DOTA-derivatised peptide ligands. The system comprises concentration and purification of the 68Ga eluate as well as labelling and purification steps for peptides, and can be used with different 68Ge/68Ga generator types. The suitability and efficiency were tested with two different DOTA-derivatised somatostatin derivatives and a DOTA-derivatised bombesin derivative. Amounts of 10-20 nmol of the peptides were sufficient and resulted in labelling yields of 50% for all peptides. The built-in safety precautions have proven to be appropriate in allowing use of the method for routine clinical applications. The system was set up and operated in a "hot lab" by personnel with no previous experience in the preparation of PET radiopharmaceuticals.
authors.affiliation: Klinik für Nuklearmedizin, Medizinische Hochschule Hannover, Carl Neuberg Str.1, 30623, Hannover, Germany. meyer.geerd-j@mh-hannover.de
authors.forename: G-J
authors.initials: GJ
authors.lastname: Meyer
authors.forename: H
authors.initials: H
authors.lastname: Mäcke
authors.forename: J
authors.initials: J
authors.lastname: Schuhmacher
authors.forename: W H
authors.initials: WH
authors.lastname: Knapp
authors.forename: M
authors.initials: M
authors.lastname: Hofmann
mesh_terms: D001839:Bombesin / Q000031:analogs & derivatives / Q000302:isolation & purification; D005710:Gallium Radioisotopes / Q000737:chemistry* / Q000302:isolation & purification; D006573:Heterocyclic Compounds, 1-Ring / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification; D007553:Isotope Labeling / Q000295:instrumentation* / Q000379:methods*; D008024:Ligands; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification; D013004:Somatostatin / Q000031:analogs & derivatives / Q000302:isolation & purification; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods
chemical_list: D005710:Gallium Radioisotopes; D006573:Heterocyclic Compounds, 1-Ring; D008024:Ligands; D010455:Peptides; D019275:Radiopharmaceuticals; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid; D013004:Somatostatin; D001839:Bombesin