title: Improved mass analysis of oligoribonucleotides by 13C, 15N double depletion and electrospray ionization FT-ICR mass spectrometry.
abstract: 13C, 15N doubly depleted 32-ribonucleotide was synthesized enzymatically by in vitro transcription from nucleoside triphosphates isolated from E. coli grown in a minimal medium containing 12C, 14N-enriched glucose and ammonium sulfate. Following purification and desalting by reversed-phase HPLC, buffer exchange with Microcon YM-3, and ethanol precipitation, electrospray ionization Fourier transform ion cyclotron resonance mass spectra revealed greatly enhanced abundance of monoisotopic ions (by a factor of approximately 100) and a narrower isotopic distribution with higher signal-to-noise ratio. The abrupt onset and high magnitude of the monoisotopic species promise to facilitate accurate mass measurement of RNA's.
authors.affiliation: Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida 32306, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiong
authors.forename: Kersten
authors.initials: K
authors.lastname: Schroeder
authors.forename: Nancy L
authors.initials: NL
authors.lastname: Greenbaum
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Hendrickson
authors.forename: Alan G
authors.initials: AG
authors.lastname: Marshall
mesh_terms: D002247:Carbon Isotopes; D005583:Fourier Analysis; D009587:Nitrogen Isotopes; D009843:Oligoribonucleotides / Q000032:analysis* / Q000737:chemistry*; D012265:Ribonucleotides / Q000032:analysis / Q000737:chemistry; D012492:Salts / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D009843:Oligoribonucleotides; D012265:Ribonucleotides; D012492:Salts