title: Aberrant expression and function of TCF4 in the proliferation of hepatocellular carcinoma cell line BEL-7402.
abstract: Wnt signaling pathway is essential for development and tumorigenesis, however, this signaling pathway in the progress of hepatocellular carcinoma (HCC) remains unclear. In this paper, we studied the function of human T-cell transcription factor-4 (TCF4), a key factor of Wnt signaling pathway, on the proliferation of HCC cell line. We showed that the expression of TCF4 mRNA in HCC cell line BEL-7402 was higher than that in immortalized normal liver cell line L02. Blockage of Wnt pathway by Delta-NTCF4, a dominant negative TCF4, could suppress BEL-7402 cells growth and decrease the expression of cyclin D1 and c-myc, two of target genes of Wnt pathway. On the other hand, stimulating Wnt pathway by introducing a degradation-resistant -catenin S37A could increase BEL-7402 cells proliferation. But all the treatments had no effect on L02 cells. Our data indicated that TCF4 might be another key factor in Wnt pathway involved in HCC cells proliferation and TCF4 could be an effective therapeutic target for suppressing the growth of hepatocellular cancers.
authors.affiliation: The Jiangsu Key Laboratory of Molecular Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210097, Jiangsu, China.
authors.forename: Dong Hong
authors.initials: DH
authors.lastname: Zhao
authors.forename: Jian Jun
authors.initials: JJ
authors.lastname: Hong
authors.forename: Shi Ying
authors.initials: SY
authors.lastname: Guo
authors.forename: Run Lin
authors.initials: RL
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Yuan
authors.forename: Chuan Yu
authors.initials: CY
authors.lastname: Wen
authors.forename: Kai Ya
authors.initials: KY
authors.lastname: Zhou
authors.forename: Chao Jun
authors.initials: CJ
authors.lastname: Li
mesh_terms: D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D045744:Cell Line, Tumor; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D003593:Cytoplasm / Q000737:chemistry; D003598:Cytoskeletal Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D015870:Gene Expression / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D051821:Lymphoid Enhancer-Binding Factor 1; D008856:Microscopy, Fluorescence; D009154:Mutation / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000502:physiology; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology; D013050:Spectrometry, Fluorescence; D051820:TCF Transcription Factors; D015534:Trans-Activators / Q000032:analysis / Q000235:genetics / Q000502:physiology; D058118:Transcription Factor 7-Like 2 Protein; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D051153:Wnt Proteins; D051176:beta Catenin
chemical_list: C495270:CTNNB1 protein, human; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; D051821:Lymphoid Enhancer-Binding Factor 1; C489427:MYC protein, human; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D051820:TCF Transcription Factors; C544894:TCF7L2 protein, human; D015534:Trans-Activators; D058118:Transcription Factor 7-Like 2 Protein; D014157:Transcription Factors; D051153:Wnt Proteins; D051176:beta Catenin; D019938:Cyclin D1