[Identification and registration of corn genotypes using molecular markers].
In plant genetics and breeding, second-generation molecular markers allow detailed characterization of plant genotypes. Unique genotypes at ten simple sequence repeat (SSR) loci were established for 40 maize accessions by means of PCR. For every locus, SSR analysis revealed heterozygotes among simple hybrids, which made it possible to identify the parental forms with a high probability of exclusion of nonparental forms. A system was proposed for registration of maize genotypes in the form of genetic formulas reflecting the allelic state of microsatellite loci, in order to catalog, preserve, and effectively employ the existing maize gene pool in breeding.
Kozhukhova
N E
NE
South Biotechnological Center of Plant Breeding, Ukrainian Academy of Agricultural Sciences, Odessa, 65036 Ukraine.
Sivolap
Iu M
IuM
D001483:Base Sequence; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D005838:Genotype; D003313:Zea mays / Q000235:genetics*
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers