title: Human cytomegalovirus (HCMV) infection in osteosarcoma cell line suppresses GM-CSF production by induction of TGF-beta.
abstract: This study was performed to elucidate the possible mechanism of the disturbance of hemopoiesis by HCMV infection. Saos-2 cells constitutively express mRNA of GM-CSF, and its expression was profoundly decreased by HCMV infection, which required full replication of the virus and was mediated by soluble factors released from the HCMV-infected Saos-2 cells. TGF-beta1 production was statistically and significantly increased from one day after HCMV infection. Expression and production of GM-CSF in Saos-2 cells were restored when a culture supernatant of HCMV-infected Saos-2 cells was reacted with neutralizing anti-TGF-beta antibody. Conclusively, HCMV inhibits GM-CSF expression in Saos-2 cells partly by the increased production of TGF-beta1.
authors.affiliation: Department of Microbiology and Immunology, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Republic of Korea.
authors.forename: Ye Jin
authors.initials: YJ
authors.lastname: Kwon
authors.forename: Dae-Joong
authors.initials: DJ
authors.lastname: Kim
authors.forename: Jung Heon
authors.initials: JH
authors.lastname: Kim
authors.forename: Chung-Gyu
authors.initials: CG
authors.lastname: Park
authors.forename: Chang-Yong
authors.initials: CY
authors.lastname: Cha
authors.forename: Eung-Soo
authors.initials: ES
authors.lastname: Hwang
mesh_terms: D002460:Cell Line; D003587:Cytomegalovirus / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D006801:Humans; D012516:Osteosarcoma / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000502:physiology*; D014407:Tumor Cells, Cultured / Q000821:virology*; D014779:Virus Replication
chemical_list: D016212:Transforming Growth Factor beta; D016178:Granulocyte-Macrophage Colony-Stimulating Factor