Different responses of macrophages to smooth and rough Brucella spp.: relationship to virulence.
By comparing smooth wild-type Brucella strains to their rough mutants, we show that the lipopolysaccharide (LPS) O side chain of pathogenic Brucella has a dramatic impact on macrophage activation. It favors the development of virulent Brucella by preventing the synthesis of immune mediators, important for host defense. We conclude that this O chain property is firmly linked to Brucella virulence.
Jiménez de Bagüés
María P
MP
Unidad de Sanidad Animal, Servicio de Investigación Agroalimentaria, Diputación General de Aragón, 50080 Zaragoza, Spain. mpjimenezdebagues@aragob.es
Terraza
Annie
A
Gross
Antoine
A
Dornand
Jacques
J
D000818:Animals; D017347:Brucella melitensis / Q000145:classification / Q000235:genetics / Q000472:pathogenicity*; D041021:Brucella suis / Q000145:classification / Q000235:genetics / Q000472:pathogenicity*; D002006:Brucellosis / Q000276:immunology / Q000382:microbiology; D002460:Cell Line; D008070:Lipopolysaccharides / Q000276:immunology; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology*; D051379:Mice; D009154:Mutation; D009569:Nitric Oxide / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014774:Virulence
D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide