Subcellular distribution of Bcl-2 family proteins and 14-3-3 within the hippocampus during seizure-induced neuronal death in the rat.
The molecular regulation of seizure-induced neuronal death may involve interactions between proteins of the Bcl-2 and 14-3-3 families. To further examine these pathways we performed subcellular fractionation on hippocampi obtained following a brief period of status epilepticus in the rat. Western blotting determined seizures induced caspase-8 cleavage and increased Bcl-w levels within the cytoplasm. Bax, Bad and Bid were largely present within the cytoplasm before and after seizures, although some Bax and, following seizures, truncated Bid was detected in mitochondria. Levels of 14-3-3 were significantly reduced in the cytoplasm and microsomal fractions. These data establish the expression and distribution profile of key Bcl-2 family proteins and the signaling chaperone 14-3-3 in the rat and provide additional evidence for the activation of programmed cell death pathways by seizures.
Schindler
Clara K
CK
Robert S. Dow Neurobiology Laboratories, Legacy Clinical Research and Technology Center, 1225 NE 2nd Avenue, Portland, OR 97232, USA.
Shinoda
Sachiko
S
Simon
Roger P
RP
Henshall
David C
DC
D048948:14-3-3 Proteins; D000818:Animals; D051023:BH3 Interacting Domain Death Agonist Protein; D015153:Blotting, Western / Q000379:methods; D002352:Carrier Proteins / Q000378:metabolism; D053181:Caspase 8; D020169:Caspases / Q000378:metabolism; D016923:Cell Death / Q000502:physiology*; D003593:Cytoplasm / Q000378:metabolism; D007839:Functional Laterality; D006624:Hippocampus / Q000378:metabolism* / Q000503:physiopathology; D007608:Kainic Acid; D008297:Male; D008861:Microsomes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D013347:Subcellular Fractions / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*; D051028:bcl-2-Associated X Protein; D051025:bcl-Associated Death Protein
D048948:14-3-3 Proteins; D051023:BH3 Interacting Domain Death Agonist Protein; C494821:Bad protein, rat; C494828:Bax protein, rat; C494816:Bid protein, rat; D002352:Carrier Proteins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D051025:bcl-Associated Death Protein; D014446:Tyrosine 3-Monooxygenase; C505528:Casp8 protein, rat; D053181:Caspase 8; D020169:Caspases; D007608:Kainic Acid