Comparative genomic analysis of the sigB operon in Listeria monocytogenes and in other Gram-positive bacteria.
The stress-responsive, alternative sigma factor sigmaB has been described in members of three Gram-positive genera, Bacillus, Listeria, and Staphylococcus. In these bacteria, sigmaB appears to play an important role in facilitating rapid adaptation to and survival in stressful environments. sigmaB activity is regulated through a complex system of phosphatases and kinases encoded by rsb (regulator of sigma B) genes. We describe the sigB operon structure for the facultative intracellular pathogen Listeria monocytogenes and apply this sequence as well as other previously described sigB operon sequences to probe the evolution and functional conservation of the sigmaB stress response system among different Gram-positive bacteria. While sigmaB as well as two Rsbs (RsbS and RsbT) are highly conserved (73%, 84%, and 79% average amino acid [aa] identities, respectively), the predicted aa sequences of the other Rsb proteins showed less conservation (62-71% aa identities). Furthermore, the sigB operon structure varies among bacterial species. Bacterial species differ in the numbers and identities of rsb genes encoded in their genomes. We thus conclude that the sigmaB stress-response system as represented by the sigB operon has diverged in both its overall components as well as in the sequences of its individual proteins, even among closely related bacterial species. Differential evolution of this stress response system among various genera may represent a strategy that enables bacteria to adapt cellular response and survival systems to a variety of stress conditions.
Ferreira
Adriana
A
Department of Food Science, Cornell University, 413 Stocking Hall, Ithaca, NY 14853, USA.
Gray
Michael
M
Wiedmann
Martin
M
Boor
Kathryn J
KJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D017124:Conserved Sequence; D019143:Evolution, Molecular; D016680:Genome, Bacterial; D008089:Listeria monocytogenes / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012808:Sigma Factor / Q000235:genetics*
C480945:SigB protein, Listeria monocytogenes; D012808:Sigma Factor