[Cloning and sequence analysis of the preprotein cDNA of human bone morphogenetic protein 12].
OBJECTIVE
To clone the cDNA of the preprotein cDNA of human bone morphogenetic protein 12 (hBMP12).


METHODS
Two primers were designed according to hBMP12 sequence reported in GenBank. The hBMP12 preprotein cDNA was obtained by reverse transcriptional (RT)-PCR from the mRNA extracted from human placenta, followed by cloning into pTARGETTM plasmid and sequence analysis of the plasmid pT(ARGE)T(TM)-BMP12.


RESULTS
DNA agarose gel electrophoresis showed that the product of RT-PCR was about 920 bp, as was consistent with the result of PCR detection of the recombinant plasmid. The result of sequence analysis was in agreement with the reported hBMP12 sequence in GenBank.


CONCLUSIONS
The preprotein of hBMP12 cDNA has been successfully cloned with correct sequence.
Lu
Yan-meng
YM
Central Laboratory, First Military Medical University, Guangzhou 510515, China. tse@fimmu.com
Fu
Wen-yu
WY
Piao
Ying-jie
YJ
D001483:Base Sequence; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D055412:Growth Differentiation Factors; D006801:Humans; D011498:Protein Precursors / Q000235:genetics*; D011994:Recombinant Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016212:Transforming Growth Factor beta
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D018076:DNA, Complementary; C499470:GDF7 protein, human; D055412:Growth Differentiation Factors; D011498:Protein Precursors; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C086559:growth differentiation factor 7; C499469:recombinant human bone morphogenetic protein-2