The occurrence of chromosome segregational defects is an intrinsic and heritable property of oral squamous cell carcinoma cell lines.
Chromosomal segregational defects are commonly observed in cancer cells and are an important source of genetic instability. It is currently unknown whether these mitotic defects are the result of a subpopulation of defective cells or reflect characteristics of the population of cells as a whole. In this study, we compared chromosomal segregational defects in two oral squamous cell carcinoma cell lines and five single-cell clones from each of those cell lines. We used immunofluorescence microscopy to quantitate the occurrence of multipolar metaphase spindles, lagging chromosomes at metaphase and anaphase, and anaphase bridges. We conclude that chromosome segregational defects in these cancer cell lines represent an intrinsic and inherited tendency toward segregational defects in the general cell population, rather than the existence of a subpopulation of cells with segregational defects.
Reing
Janet E
JE
Department of Biological Sciences, University of Pittsburgh and the University of Pittsburgh Cancer Institute, Pittsburgh, PA 15260, USA.
Gollin
Susanne M
SM
Saunders
William S
WS
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D043171:Chromosomal Instability; D020090:Chromosome Segregation; D006801:Humans; D007621:Karyotyping; D008856:Microscopy, Fluorescence; D008938:Mitosis / Q000235:genetics; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D008941:Spindle Apparatus / Q000235:genetics; D014407:Tumor Cells, Cultured