Cloning and partial characterization of a beta-1,3-glucanase gene from strawberry.
Plant pathogenesis-related proteins, including beta-1,3-glucanses, are believed to be involved in plant defense mechanisms. We have cloned a beta-1,3-glucanse gene from strawberry (Fragaria x ananassa Duch). This gene, designated FaBG2-1, is a Class II glucanase gene composed of two exons and one intron. The location of a 397-base intron in the gene was confirmed by sequencing a partial cDNA clone obtained by using a rapid amplification of cDNA ends procedure. Also, based on the cDNA sequence, the transcription start site of FaBG2-1 was assigned to a -54G residue. Genomic Southern blot analysis indicated that FaBG2-1 is a member of a multi-gene family. Reverse transcriptase-polymerase chain reaction analysis revealed that this beta-1,3-glucanase gene is expressed constitutively in strawberry leaves.
Khan
Anwar A
AA
Department of Biological Sciences, Louisiana State University, LSU Agricultural Center, Baton Rouge, LA 70803, USA.
Shi
Yanlin
Y
Shih
Ding S
DS
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D031985:Fragaria / Q000201:enzymology / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D043326:Glucan 1,3-beta-Glucosidase / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D043326:Glucan 1,3-beta-Glucosidase