In vivo topical anti-inflammatory and in vitro antioxidant activities of two extracts of Thymus satureioides leaves.
Four extracts at increasing polarity were prepared from the leaves of Thymus satureioides Coss. (Labiatae) and assayed for the in vivo topical anti-inflammatory effect using the croton oil ear test in mice, and for in vitro both antioxidant (DPPH degrees test) and anti-bacterial (broth microdilution method) activities. The chloroform extract showed a topical anti-inflammatory activity (ID50=282 microg cm(-2)), only three times lower than that of the reference drug indomethacin (ID50=93 microg cm(-2)) and its active components were identified as ursolic and oleanolic acids. The methanol extract, showing a significant radical-scavenging effect (SC50=14.54 microg), was characterized by the isolation and identification of some flavonoids. On the contrary, the extracts did not show any anti-bacterial effect against four standard aerobial bacteria strains.
Ismaili
H
H
Faculté de Médecine et Pharmacie, Université Mohammed V, Rabat, Morocco.
Milella
L
L
Fkih-Tetouani
S
S
Ilidrissi
A
A
Camporese
A
A
Sosa
S
S
Altinier
G
G
Della Loggia
R
R
Aquino
R
R
D000287:Administration, Topical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D007249:Inflammation / Q000188:drug therapy*; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; D014315:Triterpenes