title: Induction of myeloperoxidase and nitrotyrosine formation in a human eosinophilic leukemia cell line, EoL-1.
abstract: The human eosinophilic leukemia cell line, EoL-1, differentiated with butyrate as an eosinophilic cellular model was evaluated for peroxidase-dependent tyrosine nitration. Butyrate suppressed cell growth and induced eosinophilic granules in EoL-1 cells after 9 days of culture. Peroxidase activity was detected biochemically and histochemically from 3-day cultures and it increased in a time dependent manner. This peroxidase activity was inhibited by cyanide. Nitrotyrosine formation catalysed by peroxidase using hydrogen peroxide and nitrite was detected at a high level similar to that of mature eosinophils. However, no expression of eosinophil peroxidase (EPO) was detected by RT-PCR or immunocytochemistry. In contrast, the induction of myeloperoxidase (MPO) by butyrate was clearly detected by RT-PCR, Northern blot, and immunocytochemical staining. These results suggest that butyrate induces MPO rather than EPO in EoL-1 cells and that the formation of nitrotyrosine in butyrate-induced cells is dependent on MPO.
authors.affiliation: Department of Environmental and Preventive Medicine, Graduate School of Medical Science, Kanazawa University, 13-1 Takara-machi, 920-8640 Kanazawa, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Kodama
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kambayashi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kubo
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Nobukuni
authors.forename: Shingo
authors.initials: S
authors.lastname: Kimura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakamura
authors.forename: Keiki
authors.initials: K
authors.lastname: Ogino
mesh_terms: D002087:Butyrates / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003594:Cytoplasmic Granules / Q000187:drug effects; D047209:Eosinophil Peroxidase; D006801:Humans; D017681:Hypereosinophilic Syndrome / Q000188:drug therapy / Q000201:enzymology* / Q000378:metabolism; D007150:Immunohistochemistry; D009195:Peroxidase / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000096:biosynthesis*
chemical_list: D002087:Butyrates; C002744:3-nitrotyrosine; D014443:Tyrosine; D047209:Eosinophil Peroxidase; D010544:Peroxidases; D009195:Peroxidase