Extracellular calcium and magnesium, but not iron, are needed for optimal growth of Blastomyces dermatitidis yeast form cells in vitro.
In the present study, we demonstrate that the yeast form of Blastomyces dermatitidis can proliferate for short periods of time in the absence of ferric iron but not in the absence of calcium or magnesium. The results of this study shed light on the resistance of B. dermatitidis to chelating agents, such as deferoxamine, and may explain how B. dermatitidis resists the iron-binding activity of serum transferrin.
Giles
Steven S
SS
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Czuprynski
Charles J
CJ
D001758:Blastomyces / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D017610:Calcium Compounds / Q000378:metabolism* / Q000494:pharmacology; D003470:Culture Media; D003676:Deferoxamine / Q000494:pharmacology; D066298:In Vitro Techniques; D007501:Iron / Q000378:metabolism / Q000494:pharmacology; D007502:Iron Chelating Agents / Q000494:pharmacology; D008278:Magnesium Sulfate / Q000378:metabolism* / Q000494:pharmacology; D009566:Nitrates / Q000378:metabolism* / Q000494:pharmacology
D017610:Calcium Compounds; D003470:Culture Media; D007502:Iron Chelating Agents; D009566:Nitrates; D008278:Magnesium Sulfate; D007501:Iron; D003676:Deferoxamine; C059948:calcium nitrate