A novel fungal prenyl diphosphate synthase in the dimorphic zygomycete Mucor circinelloides.
Two Mucor circinelloides structural genes involved in isoprenoid biosynthesis were isolated and characterised. The isoA gene encodes a typical eukaryotic farnesyl diphosphate synthase (EC 2.5.1.10), whereas the isoB gene deduced amino acid sequence shows similarity to fungal medium-chain prenyl diphosphate synthases. By functional complementation in Escherichia coli, the isoB gene product was shown to be a solanesyl diphosphate synthase (EC 2.5.1.11), which is the first fungal enzyme reported having this specificity. In addition, a M. circinelloides one-marker-per-chromosome map was completed by contour-clamped homogeneous electric field localisation of isoA, isoB and three other isoprenoid biosynthesis genes to individual chromosomes.
Velayos
Antonio
A
Area de Genética, Departamento de Microbiología y Genética, Universidad de Salamanca, Edificio Departamental, Lab 301-2, Avenida Campo Charro s/n, 37007 Salamanca, Spain.
Fuentes-Vicente
Mónica
M
Aguilar-Elena
Raúl
R
Eslava
Arturo P
AP
Iturriaga
Enrique A
EA
D019883:Alkyl and Aryl Transferases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001483:Base Sequence; D002875:Chromosomes / Q000235:genetics*; D003001:Cloning, Molecular; D011756:Diphosphates / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005816:Genetic Complementation Test; D009089:Mucor / Q000201:enzymology* / Q000235:genetics; D011106:Polyisoprenyl Phosphates / Q000096:biosynthesis; D017422:Sequence Analysis, DNA
D011756:Diphosphates; D005656:Fungal Proteins; D011106:Polyisoprenyl Phosphates; D019883:Alkyl and Aryl Transferases; C022644:trans-octaprenyltranstransferase