Application of inter-simple sequence repeats to insect cell lines: identification at the clonal and tissue-specific level.
Inter-simple sequence repeat (ISSR) primers designed to anneal to microsatellites were used to obtain deoxyribonucleic acid (DNA) fingerprint profiles to distinguish among 16 established insect cell lines derived from an assortment of lepidopteran, dipteran, and coleopteran species. Three different levels of cell line comparison were made: (1) between parents and their clones, (2) among cell lines derived from different tissues from the same species, and (3) among cell lines derived from different insect species. Of the 16 repeat oligonucleotide primers used in this study, nine primers generated several unique markers to distinguish between parental cell lines and their clones. Four of the 16 primers also generated DNA profiles with a number of unique bands, enabling the distinction among cell lines derived from specific tissues from the same species. In addition, ISSR-generated DNA profiles provided the greatest number of unique markers to distinguish easily among insect cell lines derived from different species.
Grasela
James J
JJ
Biological Control of Insects Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, 1503 S. Providence Road, Research Park, Columbia, Missouri 65203-3535, USA. graselajj@missouri.edu
McIntosh
Arthur H
AH
D000818:Animals; D002460:Cell Line; D016172:DNA Fingerprinting / Q000379:methods; D017931:DNA Primers / Q000235:genetics*; D005819:Genetic Markers; D007313:Insecta / Q000145:classification; D018895:Microsatellite Repeats; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D005819:Genetic Markers