title: Novel ligands for the investigation of imidazoline receptors and their binding proteins.
abstract: New ligands for imidazoline receptors are described so that these receptors can be more fully explored and understood. BU224, (2-(4,5-dihydroimidaz-2-yl)-quinoline, shows high affinity and is selective for the imidazoline-2 (I(2)) class of receptors. BU224 was tested in the rat Porsolt forced swim paradigm where it was found to decrease time spent immobile and increase the time spent swimming, consistent with an antidepressant profile. BU224 was tritiated and, in radioligand binding studies, was found to label a single population of saturable sites with high affinity. In vitro brain autoradiography with [(3)H]BU224 also showed a pattern of distribution similar to the known labeling of I(2) receptors. A new series of four 2BFI (2-(benzofuranyl)-2-imidazoline) derivatives were investigated as potential ligands for imaging brain I(2) receptors using positron emission tomography (PET). At least two, BU20012 and BU20013, retained high affinity and moderate selectivity and penetrated the brain when administered peripherally in the mouse. 2BFI has undergone the Mannich reaction to immobilized diaminodipropyl amine to fabricate an affinity column, which was used to isolate a protein from rabbit brain; this protein was sequenced and identified as the enzyme creatine kinase.
authors.affiliation: Psychopharmacology Unit, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK. a.l.hudson@bristol.ac.uk
authors.forename: A L
authors.initials: AL
authors.lastname: Hudson
authors.forename: R J
authors.initials: RJ
authors.lastname: Tyacke
authors.forename: M D
authors.initials: MD
authors.lastname: Lalies
authors.forename: N
authors.initials: N
authors.lastname: Davies
authors.forename: D P
authors.initials: DP
authors.lastname: Finn
authors.forename: O
authors.initials: O
authors.lastname: Marti
authors.forename: E
authors.initials: E
authors.lastname: Robinson
authors.forename: S
authors.initials: S
authors.lastname: Husbands
authors.forename: M C W
authors.initials: MC
authors.lastname: Minchin
authors.forename: A
authors.initials: A
authors.lastname: Kimura
authors.forename: D J
authors.initials: DJ
authors.lastname: Nutt
mesh_terms: D000818:Animals; D001345:Autoradiography; D001522:Behavior, Animal / Q000502:physiology; D001923:Brain Chemistry; D001927:Brain Diseases / Q000378:metabolism; D006801:Humans; D007093:Imidazoles / Q000737:chemistry / Q000378:metabolism*; D054755:Imidazoline Receptors; D008024:Ligands; D051379:Mice; D015394:Molecular Structure; D011817:Rabbits; D011869:Radioligand Assay; D051381:Rats; D018341:Receptors, Adrenergic, alpha-2 / Q000378:metabolism; D011955:Receptors, Drug / Q000378:metabolism*; D014055:Tomography, Emission-Computed; D014316:Tritium / Q000378:metabolism
chemical_list: C105826:BU 224; D007093:Imidazoles; D054755:Imidazoline Receptors; D008024:Ligands; D018341:Receptors, Adrenergic, alpha-2; D011955:Receptors, Drug; D014316:Tritium