[HIV Antigens as complement fixing circulating immune complexes].
To detect HIV antigens in circulating complement fixing immune complexes (ICs) we assessed an ELISA using wells of microtitre plates coated with F(ab)2 anti-C3b and monoclonal antibodies anti-HIV gp120 and anti-HIV p24. We tested 24 anti-HIV positive subjects (Group A), 10 anti-HIV negative subjects at risk of acquiring HIV infection (Group B), 20 normal controls (Group C) and 2 seroconversion panels. We found HIV antigens in ICs in all sera from seroconversion panels, in 25.5% of sera from subjects in Group A, in 28.6% of sera from subjects in Group B and in no serum from subjects in Group C. A subject in Group B acquired HIV infection during the observation. HIV antigens in ICs by our assay were detected 8 months before Anti-HIV and Ag by commercial ELISA.
Filippini
P
P
Clinica Malattie Tropicali e Subtropicali, Seconda Universita di Napoli, Italy.
Guarino
M
M
Lapenta
C
C
Marrocco
C
C
Panico
L
L
Messina
V
V
Piccinino
F
F
Sagnelli
E
E