Metal-chelating properties, electrochemical behavior, scavenging and cytoprotective activities of six natural phenolics.
Chelation, electrochemical, antioxidant and cytoprotective properties of six phenolics - cynarin and caffeic, chlorogenic, ferulic, protocatechuic and rosmarinic acids were studied on the following models: (i) chelation of transition metals, (ii) quenching of the diphenylpicrylhydrazyl radical (DPPH), (iii) determination of half-wave potential, (iv) erythrocytes or mitochondrial membranes damaged by tert-butyl hydroperoxide (tBH) and (v) a primary culture of rat hepatocytes intoxicated by Cu(II) and Fe(III) or tBH. All phenolics suppressed cell membrane damage induced by transition metals or tBH. The protectivity correlated with their capacity to bind transition metals, to scavenge DPPH radical and with the value of half-wave potentials. In in vitro assays, the most promising was rosmarinic acid.
Psotová
Jitka
J
Institute of Medical Chemistry and Biochemistry, Faculty of Medicine, Palacký University, Hnevotínská 3, 775 15 Olomouc, Czech Republic. psotova@tunw.upol.cz
Lasovský
Jan
J
Vicar
Jaroslav
J
D000818:Animals; D001713:Biphenyl Compounds / Q000737:chemistry; D002478:Cells, Cultured; D002614:Chelating Agents / Q000737:chemistry*; D019610:Cytoprotection; D004912:Erythrocytes / Q000187:drug effects; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects; D022781:Hepatocytes / Q000187:drug effects; D006834:Hydrazines / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D001713:Biphenyl Compounds; D002614:Chelating Agents; D016166:Free Radical Scavengers; D006834:Hydrazines; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl