Cell cycle-dependent regulation of the human aurora B promoter.
Aurora B is an important regulator of mitosis, and its mRNA and protein levels are tightly regulated during the cell cycle. In this study, we cloned the 5' flanking region of the human aurora B gene and characterized its promoter activity. Two major transcription initiation sites were identified by primer extension. aurora B promoter activity was upregulated during M phase, and its cell cycle-dependent element (CDE) and cell cycle-gene homology region (CHR) upstream of the transcription initiation sites regulated the cell cycle-dependent promoter activity. Several CDE-binding protein complexes were identified using the electrophoretic mobility shift assay. Using the biotin-streptavidin pull-down assay, binding of E2F-1, E2F-4, and DP-2, but not of DP-1, to the CDE was detected. These results demonstrate that aurora B mRNA level is regulated by CDE-CHR and that a subset of E2F/DP family proteins binds to the CDE.
Kimura
Masashi
M
Department of Molecular Pathobiochemistry, Gifu University School of Medicine, Tsukasamachi-40, Gifu 500-8705, Japan. yo@cc.gifu-u.ac.jp
Uchida
Chiharu
C
Takano
Yukihiko
Y
Kitagawa
Masatoshi
M
Okano
Yukio
Y
D064107:Aurora Kinase B; D064127:Aurora Kinases; D001483:Base Sequence; D001710:Biotin / Q000378:metabolism; D002453:Cell Cycle; D018797:Cell Cycle Proteins; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D050684:E2F Transcription Factors; D050687:E2F1 Transcription Factor; D050704:E2F4 Transcription Factor; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D008938:Mitosis; D008969:Molecular Sequence Data; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019809:Streptavidin / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection; D015854:Up-Regulation
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human; D050704:E2F4 Transcription Factor; C493762:E2F4 protein, human; D012333:RNA, Messenger; C094103:TFDP2 protein, human; D014157:Transcription Factors; D001710:Biotin; D019809:Streptavidin; D008156:Luciferases; C576921:AURKB protein, human; D064107:Aurora Kinase B; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases