title: Use of bifunctional cross-linking reagents in mapping genomic distribution of chromatin remodeling complexes.
abstract: Chromatin remodeling complexes consist of multiple subunits, some of which are in intimate contact with DNA while others are not. The ability to effectively cross-link proteins to DNA with formaldehyde is impacted by the average distance between the two species. Productive cross-linking of proteins not in direct contact with DNA is greatly facilitated by the inclusion of an initial cross-linking reaction using bifunctional imidoester cross-linking reagents.
authors.affiliation: Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Whitehead Building, Room 142, 615 Michael Street, Atlanta, GA 30322, USA.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Fujita
authors.forename: Paul A
authors.initials: PA
authors.lastname: Wade
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002843:Chromatin / Q000235:genetics* / Q000502:physiology; D042002:Chromatin Assembly and Disassembly / Q000235:genetics* / Q000502:physiology; D002874:Chromosome Mapping / Q000379:methods*; D003432:Cross-Linking Reagents; D023281:Genomics / Q000379:methods*; D006801:Humans; D011401:Promoter Regions, Genetic
chemical_list: D002843:Chromatin; D003432:Cross-Linking Reagents