title: Isolation and characterization of a strain of Bacillus thuringiensis subsp. morrisoni PG-14 encoding delta-endotoxin Cry1Ac.
abstract: Bacillus thuringiensis 656-3, isolated from a soil sample collected at mushroom houses, showed high toxicity to mushroom flies, Lycoriella mali and Coboldia fuscipes. B. thuringiensis 656-3 produced bipyramidal inclusions and reacted with the H antiserum of B. thuringiensis subsp. morrisoni (H8a8b). The plasmid and protein profiles of B. thuringiensis 656-3 were similar to those of its reference strain, subsp. morrisoni PG-14. However, PCR analysis using cry gene primers showed that B. thuringiensis 656-3, unlike its reference strain, had cry4A, cry4B, cry10A, cry11A, and cry1Ac genes, suggesting that B. thuringiensis 656-3 was a unique strain with respect to gene type. In addition, B. thuringiensis 656-3 showed a high level of toxicity against mushroom flies, L. mali and C. fuscipes.
authors.affiliation: Division of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Korea.
authors.forename: Yong Soo
authors.initials: YS
authors.lastname: Choi
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Cho
authors.forename: Yeon Ho
authors.initials: YH
authors.lastname: Je
authors.forename: Jong Yul
authors.initials: JY
authors.lastname: Roh
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Chang
authors.forename: Ming Shun
authors.initials: MS
authors.lastname: Li
authors.forename: Sook Jae
authors.initials: SJ
authors.lastname: Seo
authors.forename: Hung Dae
authors.initials: HD
authors.lastname: Sohn
authors.forename: Byung Rae
authors.initials: BR
authors.lastname: Jin
mesh_terms: D000363:Agaricales; D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism / Q000648:ultrastructure; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001427:Bacterial Toxins; D001681:Biological Assay; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004175:Diptera / Q000378:metabolism; D004731:Endotoxins / Q000235:genetics* / Q000378:metabolism; D006460:Hemolysin Proteins; D008855:Microscopy, Electron, Scanning; D010572:Pest Control, Biological; D016133:Polymerase Chain Reaction; D012988:Soil Microbiology
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis