title: Rabbit knee model of post-traumatic joint contractures: the long-term natural history of motion loss and myofibroblasts.
abstract: Our objective is to describe the natural history of motion loss with time and myofibroblast numbers in a rabbit knee model of post-traumatic joint contractures. Twenty-eight skeletally mature New Zealand White female rabbits had five-mm-squares of cortical bone removed from the medial and lateral femoral condyles of the right knee. A Kirschner wire (K-wire) was used to immobilize the knee joint in maximum flexion. A second operation was performed 8 weeks later to remove the K-wire. The rabbits were divided into four groups depending on the time of remobilization; 0, 8, 16 or 32 weeks. The average flexion contracture of the experimental knees in the 0-week and 8-week remobilization groups (38 degrees and 33 degrees, respectively) were significantly greater when compared with the values of the unoperated contralateral knees (8 degrees). The average flexion contractures of the experimental knees in the 16-week and 32-week remobilization groups were also greater than the unoperated contralateral knees, although they were not statistically significant. The average flexion contractures of the 16-week and 32-week groups were 19 degrees and 18 degrees, respectively, indicating a stabilization of the motion loss. Myofibroblast numbers in the posterior joint capsules were elevated 4-5x in the knees with contractures when compared to the contralateral knees. The initial decrease in severity followed by stabilization of motion loss and the association of motion loss with myofibroblasts mimics the human scenario of permanent post-traumatic joint contractures.
authors.affiliation: Department of Surgery, University of Calgary, 3330 Hospital Dr NW, Calgary, AB, Canada T2N 4N1. hildebrk@ucalgary.ca
authors.forename: Kevin A
authors.initials: KA
authors.lastname: Hildebrand
authors.forename: Craig
authors.initials: C
authors.lastname: Sutherland
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D003286:Contracture / Q000209:etiology / Q000473:pathology* / Q000503:physiopathology; D004195:Disease Models, Animal; D005260:Female; D005264:Femoral Fractures / Q000150:complications / Q000473:pathology / Q000503:physiopathology; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology*; D017102:Fracture Healing; D019417:Hindlimb Suspension; D007150:Immunohistochemistry; D007797:Laminin / Q000378:metabolism; D018485:Muscle Fibers, Skeletal / Q000378:metabolism / Q000473:pathology*; D011817:Rabbits; D016059:Range of Motion, Articular; D013264:Stifle / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology
chemical_list: D000199:Actins; D007797:Laminin