[Study on the anti-apopotosis induced by hypoxia/hypoglycemia and reoxygenation of panax notoginseng saponins in cultured rat hippocampal neurons].
OBJECTIVE
To study the inhibitory effects of Panax Notoginseng Saponins(PNS) on apoptosis induced by hypoxia/hypoglycemia and reoxygenation in cultured rat hippocampal neurons.


METHODS
Apoptosis were measured by flow cytometry, intracellular free calcium concentration([Ca2+]i) was measured with confocal laser scanning microscopy, morphological changes and neuronal necrosis were observed with fluorescence microscope, and meanwhile the leakage of lactic dehydrogenase(LDH) was measured.


RESULTS
Hypoxia/hypoglycemia cultures for 5 hours and reoxygenation induced neuronal apoptosis and necrosis, and significantly increased neuronal [Ca2+]i and the leakage of LDH. The effects were increased with the extending time of reoxygenation. PNS has could significantly decrease the percentage of neuronal apoptosis and necrosis, and reduce neuronal [Ca2+]i and the leakage of LDH.


CONCLUSIONS
PNS has inhibitory effect on neuronal apoptosis. This effect might be related to its effect of decreasing intracellular free calcium concentration.
Zhu
Ling-Qun
LQ
Department of Neurology, Dongzhimeng Hospital, Beijing University of Chinese Medicine, Beijing 100700, China.
Fan
Ji-ping
JP
Huang
Qi-fu
QF
Sun
Su-lun
SL
Gao
Ying
Y
Zou
Yi-huai
YH
Zhang
Zhuang
Z
He
Li-yun
LY
Zheng
Hong
H
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D015687:Cell Hypoxia; D002478:Cells, Cultured; D005333:Fetus; D036145:Ginsenosides / Q000302:isolation & purification / Q000494:pharmacology*; D006624:Hippocampus / Q000166:cytology*; D007003:Hypoglycemia / Q000473:pathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009474:Neurons / Q000166:cytology; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
D036145:Ginsenosides; D007770:L-Lactate Dehydrogenase; D002118:Calcium