[Laminin-binding protein as a cellular receptor for the equine Venezuelan encephalomyelitis virus: Report 2. Inhibition of replication of equine Venezuelan encephalomyelitis virus by blocking laminin-binding protein on the surface of Vero cells].
A possible inhibition of the Venezuelan equine encephalomyelitis (VEE) virus replication in Vero cells through the laminin-binding protein (LBP) blocking in the surface of such cells was investigated in order to verify the LBP value. It was demonstrated, on the basis of the flow scanning cytometry and FITC-labeled antibodies to LBP, that there are at least 263 thousand LBP molecules in the Vero cells' surface. Blocking of the molecules by rabbit polyclonal antibodies to 43 kD of the recombinant LBP (rLBP) was shown to inhibit the VEE virus replication in Vero cells by more than 300,000 times, which made them virtually resistant to the possibility of VEE virus infection. This also confirmed that LBP is a target-molecule for VEE virus in Vero cells with the interplay between VEE virus and LBP in the cells' surface being the initial stage of virions' penetration into the cell and of their replication inside the cell.
Bondarenko
E I
EI
Research Institute of Molecular Biology, Vector State Research Center of Virology and Biotechnology, Koltsovo, Novosibirsk Region, 630559 Russia.
Protopopova
E V
EV
Konovalova
S N
SN
Surovtsev
I V
IV
Mal'tsev
V P
VP
Loktev
V B
VB
D000818:Animals; D002522:Chlorocebus aethiops; D004666:Encephalitis Virus, Venezuelan Equine / Q000378:metabolism* / Q000502:physiology; D017474:Receptors, Laminin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects*
D017474:Receptors, Laminin; D011991:Receptors, Virus