title: Genetic relationship among gill-infecting Myxobolus species (Myxosporea) of cyprinids: molecular evidence of importance of tissue-specificity.
abstract: The importance of tissue-specificity was studied in Myxobolus spp. infecting the gills of 7 cyprinid species. The 18S rDNA of 10 Myxobolus species was amplified by optimised nested-PCR, resulting in approximately 1600 bp PCR products. Phylogenetic trees generated by distance matrix and parsimony analyses revealed 4 main groups. Muscle-infecting species all belonged to the same group, while members of 2 gill-infecting groups were clearly distinguishable on the basis of tissue-specificity, and were also recognisable by differences in spore morphology. On the basis of tissue tropism, phylogenetic relationships among the species examined indicate that genetic separation is a more ancient evolutionary feature than host-specificity.
authors.affiliation: Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, 1581 Budapest, Hungary. eedit@vmri.hu
authors.forename: Edit
authors.initials: E
authors.lastname: Eszterbauer
mesh_terms: D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D003531:Cypriniformes; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics; D056890:Eukaryota / Q000145:classification / Q000166:cytology / Q000235:genetics*; D005393:Fish Diseases / Q000235:genetics / Q000469:parasitology* / Q000473:pathology; D005880:Gills / Q000473:pathology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011529:Protozoan Infections, Animal / Q000235:genetics* / Q000473:pathology; D017422:Sequence Analysis, DNA; D013170:Spores / Q000166:cytology
chemical_list: D017931:DNA Primers; D004275:DNA, Ribosomal