Resumption of follicle growth in gilts after ovarian autografting.
The aims of the study were to evaluate autografting of porcine ovarian tissue in terms of establishment of a blood supply, follicle survival and development, commencement of oestrous cycles and endocrine patterns in this polyovular species. Experiment 1, a preliminary study on four gilts, showed that ovarian tissue slices survived the grafting procedure and re-vascularised. In Experiment 2, a further six pre-pubertal gilts had both ovaries surgically removed and two thin cortical slices of each ovary were immediately reattached to each of the ovarian pedicles. Blood samples were taken at surgery and then weekly. Two gilts were slaughtered 2 weeks after surgery and ovarian tissue recovered. The remaining four gilts underwent daily checks for behavioural oestrus until slaughter 24 weeks after surgery. All four gilts showed standing heat at least once prior to slaughter. Plasma LH and FSH concentrations increased significantly (P<0.01) by 3 days after surgery, then fell gradually, but did not return to pre-surgery levels. Progesterone concentrations showed some evidence of cyclicity in all animals. In the grafted tissue, re-vascularisation of the tissue was apparent by 2 weeks post-grafting, although no preantral or antral follicles were observed. The tissue recovered after 24 weeks contained healthy preantral and antral follicles, luteal tissue and some large cystic follicles. It is unclear whether these cysts were the result of ovarian or hypothalamic/pituitary disturbance. In conclusion, the results of this study have shown that follicle growth and resumption of cyclicity can be achieved following ovarian autografting in pigs and indicate that this will be a useful model for investigating the mechanisms that control the early stages of follicular growth and ultimately ovulation rate in this multiovular species.
Hunter
M G
MG
School of Biosciences, University of Nottingham, Sutton Bonington, Leics LE12 5RD, UK. morag.hunter@nottingham.ac.uk
Hudson
N
N
Mitchell
M
M
Walker
R M
RM
Webb
R
R
D000818:Animals; D004971:Estrus / Q000502:physiology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D005836:Genitalia, Female / Q000033:anatomy & histology; D007986:Luteinizing Hormone / Q000097:blood; D009929:Organ Size; D006080:Ovarian Follicle / Q000502:physiology*; D010053:Ovary / Q000098:blood supply / Q000502:physiology / Q000637:transplantation*; D011374:Progesterone / Q000097:blood; D013552:Swine; D014182:Transplantation, Autologous
D011374:Progesterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone