The role of Slr1443 in pilus biogenesis in Synechocystis sp. PCC 6803: involvement in post-translational modification of pilins.
We isolated a transposon-induced nonmotile mutant of the cyanobacterium Synechocystis sp. PCC 6803. The mutant was revealed to have a Tn5 insertion in the slr1443 gene that showed sequence similarity to a eukaryotic-type protein kinase. Thick pili were not observed on the mutant cell surface under the electron microscope. The slr1443 gene was not involved in transcription or translation of the pilA1 gene encoding pilin, the major component of thick pili. In the mutant, lower molecular mass pilin peptides were detected than in the wild-type. The pilin variant was not truncated at the N- or C-terminus of mature PilA1. The reduced molecular mass may have resulted from insufficient post-translational modification. The amounts of pilin variants were remarkably reduced in the periplasmic and surface fractions. The pilin variants were released into liquid media without being assembled into pili. Our finding suggests that Slr1443 plays an important role in pilus biogenesis at the level of the post-translational modification of pilin.
Kim
Young Hye
YH
Proteome Analysis Team, Korea Basic Science Institute, Daejeon 305-333, Republic of Korea.
Park
Young Mok
YM
Kim
Soo-Jung
SJ
Park
Youn-Il
YI
Choi
Jong-Soon
JS
Chung
Young-Ho
YH
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002874:Chromosome Mapping; D000458:Cyanobacteria / Q000166:cytology / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D004251:DNA Transposable Elements / Q000235:genetics; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D015151:Immunoblotting; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009154:Mutation; D019897:Periplasm / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D036781:Fimbriae Proteins; D017346:Protein Serine-Threonine Kinases