D8/17 and CD19 expression on lymphocytes of patients with acute rheumatic fever and Tourette's disorder.
D8/17, an alloantigen found on B lymphocytes, has been reported to be elevated in patients susceptible to rheumatic fever and may be associated with autoimmune types of neuropsychiatric disorders. The pediatric-autoimmune-neuropsychiatric-disorders-associated-with-streptococci model is a putative model of pathogenesis for a group of children whose symptoms of obsessive-compulsive disorder and Tourette's disorder (TD) are abrupt and may be triggered by an infection with group A streptococci. As a test of this model, we have examined D8/17 levels on the B cells of patients with TD and acute rheumatic fever (ARF) along with those on the B cells of normal controls by flow cytometry. We have utilized several different preparations of D8/17 antibody along with a variety of secondary antibodies but have been unable to show an association with an elevated percentage of D8/17-positive, CD19-positive B cells in either ARF or TD. We did find, however, that the percentages of CD19-positive B cells in ARF and TD patients were significantly elevated compared to those in normal controls. Group A streptococcal pharyngitis patients also had an elevated percentage of CD19 B cells, however. These studies failed to confirm the utility of determining the percentage of B cells expressing the D8/17 alloantigen in ARF patients or our sample of TD patients. In contrast, the percentage of CD19-positive B cells was significantly elevated in ARF and TD patients, as well as group A streptococcal pharyngitis patients, suggesting a role for inflammation and/or autoimmunity in the pathogenesis of these disorders.
Weisz
Julie L
JL
Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA.
McMahon
William M
WM
Moore
Jill C
JC
Augustine
Nancy H
NH
Bohnsack
John F
JF
Bale
James F
JF
Johnson
Michael B
MB
Morgan
Jubel F
JF
Jensen
Jenise
J
Tani
Lloyd Y
LY
Veasy
L George
LG
Hill
Harry R
HR
D000208:Acute Disease; D018941:Antigens, CD19 / Q000276:immunology / Q000378:metabolism*; D015551:Autoimmunity / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D007519:Isoantigens / Q000276:immunology / Q000378:metabolism*; D010612:Pharyngitis / Q000276:immunology; D012213:Rheumatic Fever / Q000276:immunology*; D013290:Streptococcal Infections / Q000276:immunology; D005879:Tourette Syndrome / Q000276:immunology*
D018941:Antigens, CD19; D007519:Isoantigens