title: Restriction fragment length polymorphism of L1 amplicon using Rsa 1 detects five different human papillomavirus types and their co-infections among women attending a gynaecological outpatient department.
abstract: Detection of human papillomavirus (HPV) types 6, 11, 16, 18, and 33 including co-infections among females attending gynaecological outpatient department and cancer clinics, was done by restriction fragment length polymorphism (using Rsa-1), of approximately 450bp amplicon, obtained by the amplification of the L1 region of HPV genome with consensus primers MY09/11 [Cancer Cells 7 (1989) 209]. The results were further tested with HPV type specific primers [J. Med. Virol. 29 (1989) 20]. The technique was found to be low-cost and less time consuming. The advantage of Rsa 1 over other enzymes was that it detects the five most prevalent HPV types commonly associated with warts, cervical dysplasia, and cancer.
authors.affiliation: Institute of Cytology and Preventive Oncology, Indian Council of Medical Research, Maulana Azad Medical College Campus, Bahadurshah Zafar Marg, New Delhi 110002, India.
authors.forename: Samar Husain
authors.initials: SH
authors.lastname: Naqvi
authors.forename: Saima
authors.initials: S
authors.lastname: Wajid
authors.forename: Ardhendu B
authors.initials: AB
authors.lastname: Mitra
mesh_terms: D010584:Bacteriophage phi X 174 / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D016679:Genome, Viral; D005914:Globins / Q000235:genetics; D006801:Humans; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis*; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D012749:Sexually Transmitted Diseases / Q000175:diagnosis / Q000821:virology
chemical_list: D017931:DNA Primers; D005914:Globins; D015252:Deoxyribonucleases, Type II Site-Specific; C040158:GTAC-specific type II deoxyribonucleases