Uptake of bovine IgG by first instars of the common cattle grub, Hypoderma lineatum.
The midgut of first instar Hypoderma lineatum (De Vill.) (Diptera: Oestridae) was examined for the presence of bovine immunoglobulin using immuno-gold staining in conjunction with image analysis. Newly hatched larvae, unexposed to fluids or tissues of the host, showed little or no deposit of colloidal gold particles. Midgut lumen, microvillar border, epithelial cell bodies and basement membrane of first instars, 6-7 months of age, recovered from the host oesophagus, all showed colloidal gold staining. This indicates that immuno-reactive portions of bovine immunoglobulin survive exposure to extracorporeal larval enzymes, as well as transit of the midgut, and pass through epithelial cells to enter the larval haemocoel.
Colwell
Douglas D
DD
Agriculture and Agri-Food Canada, Lethbridge Research Centre, 5403 1st Avenue South, Lethbridge, Alberta, Canada T1J 4B1. colwelld@agr.gc.ca
Leggett
Fran
F
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000469:parasitology*; D004175:Diptera / Q000502:physiology*; D006790:Host-Parasite Interactions; D007074:Immunoglobulin G / Q000032:analysis*; D007150:Immunohistochemistry; D007422:Intestines / Q000737:chemistry*; D007814:Larva
D000906:Antibodies; D007074:Immunoglobulin G