Two-dimensional electrophoretic protein profile analysis following exposure of human uroepithelial cells to occupational bladder carcinogens.
Protein biomarkers to occupational carcinogens were investigated using a transformable human uroepithelial cell system, SV-HUC.PC. SV-HUC.PC was treated with N-hydroxy-4,4'-methylene bis (2-chloroaniline) (N-OH-MOCA) or N-hydroxy-4 aminobiphenyl (N-OH-ABP). Two-dimensional gel electrophoresis of cell lysates compared protein changes across treatments. Increasing N-OH-MOCA resulted in a dose-related increase in protein spots altered. Comparing cell profiles treated with either carcinogen revealed alterations in the expression of nine proteins, identified using the TagIdent database. These demonstrated isoelectric point shift (1) or quantity change (8). Our investigation may be useful in identifying biomarkers of effects of exposure to bladder carcinogens.
Kanitz
Mary Helen
MH
Division of Applied Research and Technology, Taft Laboratory, National Institute for Occupational Safety and Health, MS/C-23, 4676 Columbia Parkway, Cincinnati, OH 45226, USA. mhk2@cdc.gov
Swaminathan
Santhanam
S
Savage
Russell E
RE
D015415:Biomarkers; D002273:Carcinogens / Q000633:toxicity*; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D004847:Epithelial Cells / Q000737:chemistry / Q000187:drug effects; D006801:Humans; D016273:Occupational Exposure; D011506:Proteins / Q000032:analysis*; D001743:Urinary Bladder / Q000737:chemistry / Q000187:drug effects*; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced*
D015415:Biomarkers; D002273:Carcinogens; D011506:Proteins