title: Antiinflammatory evaluation of alcoholic extract of galls of Quercus infectoria.
abstract: Galls of Quercus infectoria Olivier (Fagaceae) possess pleiotropic therapeutic activities, with particular efficacy against inflammatory diseases. The present study was undertaken to evaluate the effect of alcoholic extract of Q. infectoria galls on various in vivo and in vitro experimental models of inflammation. Oral administration of gall extract significantly inhibited carrageenan, histamine, serotonin and prostaglandin E2 (PGE2) induced paw oedemas, while topical application of gall extract inhibited phorbol-12-myristate-13-acetate (PMA) induced ear inflammation. The extract also inhibited various functions of macrophages and neutrophils relevant to the inflammatory response. In vitro exposure of rat peritoneal macrophages to gall extract ameliorated lipopolysaccharide (LPS) stimulated PGE2 and nitric oxide (NO) production and PMA stimulated superoxide (O2*-) production in a dose dependent manner. Gall extract also scavenged NO and O2*-. Probing into mechanism of NO inhibition in macrophages revealed gall extract to ameliorate the induction of inducible NO synthase (iNOS), respectively without any inhibitory effect on its catalytic activities even at higher concentrations. Gall extract also significantly inhibited formyl-Met-Leu-Phe (fMLP) stimulated degranulation in neutrophils. These results suggest that alcoholic extract of galls of Q. infectoria exerts in vivo antiinflammatory activity after oral or topical administration and also has the ability to prevent the production of some inflammatory mediators.
authors.affiliation: Department of Medical Elementology and Toxicology, Jamia Hamdard, Hamdard Nagar, New Delhi 110062, India.
authors.forename: Gurpreet
authors.initials: G
authors.lastname: Kaur
authors.forename: Hinna
authors.initials: H
authors.lastname: Hamid
authors.forename: Asif
authors.initials: A
authors.lastname: Ali
authors.forename: M Sarwar
authors.initials: MS
authors.lastname: Alam
authors.forename: Mohammad
authors.initials: M
authors.lastname: Athar
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D000431:Ethanol / Q000494:pharmacology / Q000627:therapeutic use; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D010941:Plant Tumors; D029963:Quercus; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D010936:Plant Extracts; D000431:Ethanol