Fourier transform infrared spectroscopy as a new tool to determine rosmarinic acid in situ.
A new procedure has been developed for the in situ FT-IR determination of rosmarinic acid (RA) in suspension cultures of Lavandula officinalis. The method involves sample preparation on ZnSe crystals or microplates from silicon, and measuring absorbance spectra between 4000 and 700 cm(-1). First derivative spectra were analysed after normalisation using partial least square (PLS) algorithm. The correlation between spectral analysis and HPLC measurements of cell extracts shows that the FT-IR procedure is suitable for qualitative and quantitative analyses of RA in cell suspension cultures.
Stehfest
Katja
K
Institute of Botany, University of Leipzig, Johannisallee 21-23, D-04103 Leipzig, Germany. stehfest@rz.uni-leipzig.de
Boese
Matthias
M
Kerns
Gerhard
G
Piry
Alexander
A
Wilhelm
Christian
C
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D002934:Cinnamates / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D003517:Cyclopentanes / Q000494:pharmacology; D053630:Depsides; D007202:Indicators and Reagents; D027523:Lavandula / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D054883:Oxylipins; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D000097123:Rosmarinic Acid
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002934:Cinnamates; D003517:Cyclopentanes; D053630:Depsides; D007202:Indicators and Reagents; D054883:Oxylipins; D000097123:Rosmarinic Acid; C011006:jasmonic acid