title: First record of Cryptosporidium infection in a raccoon dog (Nyctereutes procyonoides viverrinus).
abstract: Cryptosporidium species have been found in more than 150 species of mammals, but there has been no report in raccoon dogs. Here we found the Cryptosporidium organism in a raccoon dog, Nyctereutes procyonoides viverrinus, and identified this isolate using PCR-based diagnostic methods. Cryptosporidium diagnostic fragments of the 18S ribosomal RNA, Cryptosporidium oocyst wall protein and 70-kDa heat shock protein genes were amplified from the isolate and sequenced to reveal the phylogenetic relationships between it and other Cryptosporidium species or genotypes reported previously. The results showed that the raccoon dog isolate represented the C. parvum cattle genotype which could be a causative agent in human cryptosporidiosis.
authors.affiliation: Department of Food and Nutrition, Osaka Joshi-Gakuen Junior College, Tennoji-ku, Osaka 543-0073, Japan. matsu@ojg.ac.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Matsubayashi
authors.forename: Niichiro
authors.initials: N
authors.lastname: Abe
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Takami
authors.forename: Isao
authors.initials: I
authors.lastname: Kimata
authors.forename: Motohiro
authors.initials: M
authors.lastname: Iseki
authors.forename: Teruo
authors.initials: T
authors.lastname: Nakanishi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tani
authors.forename: Kazumi
authors.initials: K
authors.lastname: Sasai
authors.forename: Eiichiroh
authors.initials: E
authors.lastname: Baba
mesh_terms: D000818:Animals; D002335:Carnivora / Q000469:parasitology*; D003457:Cryptosporidiosis / Q000469:parasitology / Q000662:veterinary*; D016785:Cryptosporidium parvum / Q000235:genetics / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan; D018840:HSP70 Heat-Shock Proteins; D015800:Protozoan Proteins; D012337:RNA, Ribosomal, 18S