[Dose dependent effects of the combined beta-gamma-emitter 188Rhenium on the growth of human vessel wall cells].
OBJECTIVE
To evaluate the dose of (188)Re that completely suppresses growth and clonogenic activity of human aortic smooth muscle cells (haSMC) since these cells are mainly responsible for restenosis occurring after PTA. For comparison, growth and clonogenic activity of endothelial cells (EC) were investigated with corresponding doses.


METHODS
Two days after plating, haSMC and EC were incubated with (188)Re for five days. The doses applied ranged from 4 to 16 Gy. Cell growth was observed for a period of 20 days (EC) or 30 days (haSMC), respectively. Clonogenic activity was monitored over a period of 20 days for both cell lines.


RESULTS
Irradiation caused dose-depend-ent inhibition of cell growth and clonogenic activity both in haSMC and in EC. HaSMC growth was completely blocked with 8 Gy, while EC still showed some proliferation even with 16 Gy. The clonal activity of haSMC was also completely blocked with 8 Gy while EC still showed little clonal activity even with 16 Gy.


CONCLUSIONS
Cell growth of both haSMC and EC can be effectively suppressed in a dose-dependent manner. Only haSMC showed a complete growth arrest with 8 Gy while EC were able to proliferate even with 16 Gy. HaSMC colony formation was completely suppressed after application of 8 Gy, while the EC still showed colony formation activity with 16 Gy. (188)Re has some advantageous properties for intravascular irradiation in comparison to other radionuclides making it an interesting radionuclide for stent coating to prevent restenosis.
Henning
E
E
Abteilung Radiologische Diagnostik, Universitätsklinik Tübingen, Tuebingen. Eva.Henning@uni-tuebingen.de
Dittmann
H
H
Wiskirchen
J
J
Bantleon
R
R
Kehlbach
R
R
Claussen
C D
CD
Duda
S H
SH
D000800:Angioplasty, Balloon; D001011:Aorta / Q000528:radiation effects; D002455:Cell Division / Q000528:radiation effects; D002460:Cell Line; D003114:Colony-Forming Units Assay; D003251:Constriction, Pathologic / Q000517:prevention & control; D004307:Dose-Response Relationship, Radiation; D042783:Endothelial Cells / Q000166:cytology / Q000528:radiation effects*; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000254:growth & development / Q000528:radiation effects*; D011829:Radiation Dosage; D011868:Radioisotopes / Q000494:pharmacology*; D012008:Recurrence; D012211:Rhenium / Q000494:pharmacology*; D015607:Stents; D013997:Time Factors
D011868:Radioisotopes; D012211:Rhenium