[Determination of danshensu in rat serum after oral administer compound Salvia recipe].
OBJECTIVE
To investigate the absorption of the water soluble components of Salvia miltiorrhiza and establish methods to determine Danshensu in rat serum after ig compound Salvia recipe.


METHODS
Used liquid-liquid extraction to deal with the serum, then determined the sample by high-performance liquid chromatography. The conditions for HPLC were as the following: the mobile phase, CH3OH-CH3CN-0.5% CH3COOH (2.5:3.5:94) with a flow rate of 1.0 mL.min-1; detection wavelength, 281 nm; internal standard, 4-hydroxy-benzoic acid.


RESULTS
Danshensu and an unknown compound whose reserved time was little shorter than protocatechuic aldehyde were found in the rat serum after ig compound Salvia recipe, while protocatechuic aldehyde weren't found in the serum.


CONCLUSIONS
Danshensu can be absorbed into blood after ig compound Salvia recipe.
Pan
Gui-xiang
GX
Tianjin College of Traditional Chinese Medicine, Tianjin 300193, China. guixiangp@163.com
Zhang
Bo-li
BL
Gao
Xiu-mei
XM
D000818:Animals; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000493:pharmacokinetics*; D005260:Female; D007773:Lactates / Q000097:blood*; D008297:Male; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza / Q000737:chemistry
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D007773:Lactates; C035055:3,4-dihydroxyphenyllactic acid