[Slide-based multi-parametric cytometry in ENT. Perspectives for the clinic and research].
BACKGROUND
Flow cytometry is the standard method for the multi-parametric analysis of cells. However, for about a decade, an instrument has been available which analyses fluorescing cells immobilised on slides called a laser scanning cytometer (LSC). Its design, according to the principles of slide-based cytometry, promises many advantages, especially in the analysis of minimal sample volumes.


METHODS
To date, applications for cultured cells and animal models have been established. Its use for clinical purposes, however, remains to be critically evaluated. We analysed a variety of specimens obtained in our clinical routine.


RESULTS
First, the instrument's resolution was evaluated using standardised particles. This showed a very good sensitivity across a wide range of fluorescence intensities at various wavelengths. Next, diverse applications for tissue engineering, immunophenotyping, and ENT-oncology were tested. Considering its microanalytical capacities, LSC proved to be a convincing tool for clinical use. Additionally, complex structures such as bi-layers of cultured cells were analysed.


CONCLUSIONS
A broad spectrum of applications in clinical practice and research for the LSC is evident.
Gerstner
A O H
AO
Klinik und Poliklinik für HNO-Heilkunde/Chirurgie der Universität Bonn. gerstaoh@web.de
Tárnok
A
A
Bootz
F
F
D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry; D047569:Laser Scanning Cytometry / Q000295:instrumentation* / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D010036:Otolaryngology / Q000295:instrumentation* / Q000379:methods; D012106:Research / Q000295:instrumentation