title: Minimal nuclear pore complexes define FG repeat domains essential for transport.
abstract: Translocation through nuclear pore complexes (NPCs) requires interactions between receptor-cargo complexes and phenylalanine-glycine (FG) repeats in multiple FG domain-containing NPC proteins (FG-Nups). We have systematically deleted the FG domains of 11 Saccharomyces cerevisiae FG-Nups in various combinations. All five asymmetrically localized FG domains deleted together were non-essential. However, specific combinations of symmetrically localized FG domains were essential. Over half the total mass of FG domains could be deleted without loss of viability or the NPC's normal permeability barrier. Significantly, symmetric deletions caused mild reductions in Kap95-Kap60-mediated import rates, but virtually abolished Kap104 import. These results suggest the existence of multiple translocation pathways.
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University Medical Center, 3120A MRBIII, 465 21st Avenue South, Nashville, TN 37232-8240, USA.
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Strawn
authors.forename: Tianxiang
authors.initials: T
authors.lastname: Shen
authors.forename: Nataliya
authors.initials: N
authors.lastname: Shulga
authors.forename: David S
authors.initials: DS
authors.lastname: Goldfarb
authors.forename: Susan R
authors.initials: SR
authors.lastname: Wente
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000235:genetics*; D002463:Cell Membrane Permeability / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D002478:Cells, Cultured; D017353:Gene Deletion; D028884:Karyopherins / Q000235:genetics / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D022022:Nuclear Pore / Q000235:genetics / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins / Q000172:deficiency* / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics; D021381:Protein Transport / Q000235:genetics*; D020449:Repetitive Sequences, Amino Acid / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D028961:beta Karyopherins / Q000235:genetics / Q000378:metabolism
chemical_list: C101820:KAP104 protein, S cerevisiae; D028884:Karyopherins; D028861:Nuclear Pore Complex Proteins; D029701:Saccharomyces cerevisiae Proteins; D028961:beta Karyopherins