Library on a slide for bacterial comparative genomics.
BACKGROUND
We describe a novel application of microarray technology for comparative genomics of bacteria in which libraries of entire genomes rather than the sequence of a single genome or sets of genes are arrayed on the slide and then probed for the presence or absence of specific genes and/or gene alleles.


RESULTS
We first adopted a 96-well high throughput working protocol to efficiently isolate high quality genomic DNA. We then optimized conditions to print genomic DNA onto a glass slide with high density (up to 15000 spots) and to sensitively detect gene targets in each genome spot using fluorescently labeled DNA probe. Finally, we created an E. coli reference collection array and probed it for the presence or absence of the hemolysin (hly) gene using a dual channel non-competing hybridization strategy. Results from the array hybridization matched perfectly with previous tests.


CONCLUSIONS
This new form of microarray technology, Library on a Slide, is an efficient way for sharing and utilizing large strain collections in comparative genomic analyses.
Zhang
Lixin
L
Department of Epidemiology, University of Michigan School of Public Health, 109 Observatory Street, Ann Arbor, MI48109, USA. lxzhang@umich.edu
Srinivasan
Usha
U
Marrs
Carl F
CF
Ghosh
Debashis
D
Gilsdorf
Janet R
JR
Foxman
Betsy
B
D015373:Bacterial Typing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis*; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*
D004269:DNA, Bacterial