PCR-DGGE differentiation of strains of Saccharomyces sensu stricto.
A quick molecular biology method based on the polymerase chain reaction (PCR) and Denaturing Gradient Gel Electrophoresis (DGGE) was developed for distinguishing strains belonging to the Saccharomyces sensu stricto group. Differentiation was obtained between S. cerevisiae, S. paradoxus and S. bayanus / S. pastorianus although no distinction was possible between S. bayanus and S. pastorianus using the amplification of the ITS regions. The ability to distinguish between different strains of the Saccharomyces sensu stricto group could allow for a better understanding of the ecology of these species on grapes as well as in musts and wines and the method developed can be useful for the quick identification of Saccharomyces sensu stricto strains from numerous isolates.
Manzano
Marisa
M
Dipartimento di Scienze degli Alimenti, Facoltà di Agraria, University of Udine, via Marangoni 97, 33100 Udine, Italy. Marisa.Manzano@DSA.uniud.it
Cocolin
Luca
L
Longo
Benedetta
B
Comi
Giuseppe
G
D001483:Base Sequence; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D012440:Saccharomyces / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
D017931:DNA Primers