[Effects of musk glucoprotein on chemotaxis of polymorphonuclear leukocytes in vivo and in vitro].
OBJECTIVE
To investigate the effects of Musk glucoprotein on chemotaxis of Polymorphonuclear leukocytes(PMN).


METHODS
The chemotaxis of PMN in abdominal cavity in rat induced by carboxymethyl cellulose(CMC) was used as an in vivo animal model and in in vitro it was evaluated by Boyden chamber. The concentration of cytosolic free Ca2+ was quantitated with the fluorescent Ca2+ indicator Fura-2.


RESULTS
The water extract of Musk at dose of 5, 20, 80 mg.kg-1 (s.c.) significantly inhibited the chemotaxis of PMN in rat; Musk-1 at concentration of 1-100 micrograms.mL-1 can significantly inhibit the chemotaxis of rabbit PMN in vitro; Musk-1 at concentration of 1-100 micrograms.mL-1 can significantly inhibit the increase of cytosolic Ca2+ concentration in PMN of rat.


CONCLUSIONS
Part of mechanisms underlying antiinflammatory action of Musk is to inhibit the chemotaxis of PMN.
Wang
Wen-jie
WJ
Institute of Materia Medica, Chinese Academy of Medical Sciences, Chinese Peiking Union Medical College, Beijing 100050. wwenj@imm.ac.cn
Zhong
Miao
M
Guo
Ying
Y
Zhou
Long-en
LE
Cheng
Gui-fang
GF
Zhu
Xiu-yuan
XY
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D006023:Glycoproteins / Q000302:isolation & purification / Q000494:pharmacology; D008297:Male; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D009504:Neutrophils / Q000378:metabolism / Q000502:physiology*; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar
D000894:Anti-Inflammatory Agents, Non-Steroidal; D005229:Fatty Acids, Monounsaturated; D006023:Glycoproteins; D008419:Materia Medica; C008563:musk; D002118:Calcium