ICBP90 expression is downregulated in apoptosis-induced Jurkat cells.
Inhibition of apoptosis, resulting from an increase in anti-apoptotic protein, plays a fundamental role in carcinogenesis. Because ICBP90 gene expression is deregulated in cancer cells, we studied its expression in Jurkat cells under apoptotic conditions to see whether ICBP90 is involved in the regulation of apoptosis. We found that ICBP90 expression and the percentage of living cells were dose-dependently decreased in PHA and ionophore A23187-stimulated Jurkat cells, but not in THP-1 cells. These results suggest that apoptosis is dependent upon ICBP90 expression downregulation and that ICBP90 exhibits anti-apoptotic properties.
Abbady
Abdul-Qader
AQ
Institut National de la Santé et de la Recherche Médicale, UMRS 392, Faculté de Pharmacie, B.P. 60024, 74, route du Rhin, 67401 Illkirch Cedex, France.
Bronner
Christian
C
Trotzier
Marie-Aline
MA
Hopfner
Raphael
R
Bathami
Kawtar
K
Muller
Christian D
CD
Jeanblanc
Michel
M
Mousli
Marc
M
D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics*; D000001:Calcimycin / Q000494:pharmacology; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D019169:Jurkat Cells; D010835:Phytohemagglutinins / Q000494:pharmacology*; D044767:Ubiquitin-Protein Ligases
D022762:CCAAT-Enhancer-Binding Proteins; D010835:Phytohemagglutinins; D000001:Calcimycin; C406364:UHRF1 protein, human; D044767:Ubiquitin-Protein Ligases