Anti-diarrheal and spasmolytic activities and acute toxicity study of Soonkijangquebo, a herbal anti-diarrheal formula.
The anti-diarrheal and spasmolytic activities of Soonkijangquebo (SKJQB), a Korean herbal anti-diarrheal formulation, were subjected to pharmacological evaluation. SKJQB, at a dose of 50-200 mg/kg, inhibited castor oil-induced diarrhea in mice. The median effective dose (ED50) for the anti-diarrheal effect was 93 mg/kg. In isolated rabbit jejunum preparations, SKJQB produced a spasmolytic effect by the relaxation of spontaneous contractions in a dose-dependent manner. The median effective concentration (EC50) for the spasmolytic effect was 3.6 mg/ml. In isolated guinea pig ileum preparations, SKJQB also produced a spasmolytic effect by reduction of acetylcholine-induced contractions. When tested against calcium channel blockade in rabbit jejunum, SKJQB caused a dose-dependent rightward shift in the Ca2+ dose-response curves, similar to that produced by verapamil, a well-known calcium antagonist. In an acute toxicity study in Sprague-Dawley rats, the median lethal dose (LD50) of SKJQB was greater than 2000 mg/kg, and no pathological changes were noticed in macroscopic examination by necropsy of rats treated with SKJQB. Thus, SKJQB may be safely used as a spasmolytic as well as an anti-diarrheal agent.
Ryu
Seung-Duk
SD
Department of Pharmacology and Medicinal Toxicology Research Center, Inchon 402-751, South Korea.
Park
Chang-Shin
CS
Baek
Hyun-Moon
HM
Baek
Sun-Hye
SH
Hwang
Sung-Yeoun
SY
Chung
Woon-Gye
WG
D000818:Animals; D000930:Antidiarrheals / Q000009:adverse effects / Q000627:therapeutic use*; D002121:Calcium Channel Blockers / Q000009:adverse effects / Q000627:therapeutic use; D005260:Female; D006168:Guinea Pigs; D007723:Korea; D008297:Male; D008519:Medicine, Traditional; D051379:Mice; D008813:Mice, Inbred ICR; D010276:Parasympatholytics / Q000009:adverse effects / Q000627:therapeutic use*; D028321:Plant Preparations / Q000009:adverse effects / Q000302:isolation & purification / Q000627:therapeutic use; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley
D000930:Antidiarrheals; D002121:Calcium Channel Blockers; D010276:Parasympatholytics; D028321:Plant Preparations