title: Enriched gonadal migration of donor-derived gonadal primordial germ cells by immunomagnetic cell sorting in birds.
abstract: This study was conducted to evaluate whether immunomagnetic treatment could improve the retrieval and migration capacity of avian gonadal primordial germ cells (gPGCs) collected from gonads in 5.5-day-old chick and 5-day-old quail embryos, respectively. Collected gPGCs were loaded into a magnetic-activated cell sorter (MACS) after being conjugated with specific gPGC antibodies and either MACS-treated or non-treated cells in each species were subsequently transferred to the recipient embryos. MACS treatment significantly (P < 0.05) increased the population ratio of gPGCs in gonadal cells retrieved (0.74 to 33.4% in the chicken and 2.68 to 45.1% in the quail). This was due to decreased number of non-gPGCs in total cell population. MACS treatment further enhanced gonadal migration of gPGCs transferred in both species (10% vs. 80-85% in the chicken and 10-15% vs. 70-80% in the quail). Increase in the number of microinjected cells up to 600 cells/embryo did not eliminate such promoting effect. In conclusion, MACS treatment greatly increased the population ratio of avian gPGCs in gonadal cells, resulting improved gonadal migration in recipient embryos.
authors.affiliation: School of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
authors.forename: Jin Nam
authors.initials: JN
authors.lastname: Kim
authors.forename: Mi A
authors.initials: MA
authors.lastname: Kim
authors.forename: Tae Sub
authors.initials: TS
authors.lastname: Park
authors.forename: Duk Kyung
authors.initials: DK
authors.lastname: Kim
authors.forename: Hyun Jeong
authors.initials: HJ
authors.lastname: Park
authors.forename: Tamao
authors.initials: T
authors.lastname: Ono
authors.forename: Jeong Mook
authors.initials: JM
authors.lastname: Lim
authors.forename: Jae Yong
authors.initials: JY
authors.lastname: Han
mesh_terms: D000818:Animals; D001717:Birds; D002452:Cell Count; D002465:Cell Movement; D002645:Chickens; D005854:Germ Cells / Q000166:cytology* / Q000276:immunology; D006066:Gonads / Q000166:cytology* / Q000276:immunology; D018189:Immunomagnetic Separation / Q000295:instrumentation / Q000379:methods*; D011784:Quail