title: Autotransplantation of unmanipulated bone marrow into scarred myocardium is safe and enhances cardiac function in humans.
abstract: Stem cell transplants into damaged myocardium may have the potential to improve cardiac function. We investigated the safety of transplanting unmanipulated autologous bone marrow into infarcted myocardium of patients undergoing coronary bypass surgery and assessed its efficacy to improve cardiac function. Fourteen patients with one or more areas of transmural myocardial infarction were studied. Autologous bone marrow was obtained by sternal bone aspirate at the time of surgery, diluted in autologous serum at a ratio of 1:2, and then injected 1 cm apart into the mid-depth of the left ventricular scar. There were no deaths, no perioperative myocardial infarctions, and no significant ventricular arrhythmias. Dobutamine stress echocardiography demonstrated overall improvement in the global and regional left ventricular function 6 weeks and 10 months after surgery. Of 34 infarcted left ventricular segments, 11 were injected with bone marrow alone, 13 were revascularized with a bypass graft alone, and 10 received bone marrow transplantation and a bypass graft in combination. Only the left ventricle segmental wall motion score of the areas injected with bone marrow and receiving a bypass graft in combination improved at low dose and at peak dobutamine stress. These findings suggest that transplantation of unmanipulated autologous bone marrow into scar tissue of the human heart is safe and enhances cardiac function only when used in combination with myocardial revascularization. This benefit can be seen after 6 weeks of the bone marrow transplant and is maintained after 10 months of follow-up.
authors.affiliation: Department of Integrative Human Cardiovascular Physiology and Cardiac Surgery, University of Leicester, Glenfield Hospital, Leicester, LE3 9QP, UK. mg50@le.ac.uk
authors.forename: Manuel
authors.initials: M
authors.lastname: Galiñanes
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Loubani
authors.forename: Joan
authors.initials: J
authors.lastname: Davies
authors.forename: Derek
authors.initials: D
authors.lastname: Chin
authors.forename: John
authors.initials: J
authors.lastname: Pasi
authors.forename: Peter R
authors.initials: PR
authors.lastname: Bell
mesh_terms: D001145:Arrhythmias, Cardiac / Q000628:therapy; D016026:Bone Marrow Transplantation / Q000379:methods*; D006348:Cardiac Surgical Procedures / Q000379:methods*; D004280:Dobutamine; D004452:Echocardiography; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000473:pathology*; D033581:Stem Cell Transplantation / Q000379:methods*; D013997:Time Factors; D014182:Transplantation, Autologous; D016896:Treatment Outcome
chemical_list: D004280:Dobutamine