Effect of weightbearing on bone formation during distraction osteogenesis.
The effect of weightbearing on the bone formation during distraction osteogenesis was studied with 24 goats after standardized osteotomy and distraction. Twelve goats were allowed to bear weight immediately and 12 were not allowed to bear weight. With the pattern of weightbearing documented, the formation of bone was monitored with serial radiographs and the bone forming activity was studied with histologic examination and immunohistochemical study of transforming growth factor-beta 1 expression. The results of the study showed that the bone formation as depicted by radiologic assessment of callus width and mineralized tissue was significantly better in the weightbearing group. Histologic study showed more bone formation qualitatively and quantitatively in the weightbearing group. This study confirmed the beneficial effect of weightbearing activity during distraction osteogenesis. Distraction provides pulsed form stimulation by tension stress across the osteotomy site and initiates osteogenesis while the compression stress exerted by the weightbearing is continuous in daily activities and enhances new bone formation. The clinical practice of early weightbearing during distraction osteogenesis should be reinforced.
Leung
Kwok-Sui
KS
Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Shatin, Hong Kong, China. ksleung@cuhk.edu.hk
Cheung
Wing-Hoi
WH
Yeung
Hiu-Yan
HY
Lee
Kwong-Man
KM
Fung
Kwok-Pui
KP
D000818:Animals; D001707:Biopsy, Needle; D002146:Bony Callus / Q000000981:diagnostic imaging* / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D006041:Goats; D007150:Immunohistochemistry; D008297:Male; D010012:Osteogenesis / Q000502:physiology*; D019857:Osteogenesis, Distraction / Q000379:methods*; D011336:Probability; D011859:Radiography; D012680:Sensitivity and Specificity; D016212:Transforming Growth Factor beta / Q000032:analysis; D053773:Transforming Growth Factor beta1; D016474:Weight-Bearing
D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1