Label-free detection of microarrays of biomolecules by oblique-incidence reflectivity difference microscopy.
We developed an oblique-incidence reflectivity difference (OI-RD) scanning microscope for label-free imaging of microarrays of biomolecules upon solid substrates. We demonstrate that hybridization reactions in an oligonucleotide microarray fabricated upon a glass slide can be detected by such an OI-RD microscope.
Landry
J P
JP
Department of Physics, National Science Foundation Center for Biophotonics Science & Technology, University of California at Davis, Davis, California 95616, USA.
Zhu
X D
XD
Gregg
J P
JP
D001671:Biochemistry / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D008962:Models, Theoretical; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D012542:Scattering, Radiation