Determination of phytohormones of environmental impact by capillary zone electrophoresis.
A test mixture of five phytohormones [naphthaleneacetic acid (NAA), naphthoxyacetic acid (NOA), indoleacetic acid (IAA), indolebutyric acid (IBA), and indolepropionic acid (IPA)] was investigated. These compounds were cleanly separated with good resolution by capillary zone electrophoresis with a UV diode array detector using 20 mM sodium phosphate buffer (pH 7.25). The lowest detection limit was obtained for IPA (0.45 mg L(-)(1) or 0.005 mg kg(-)(1)) and the highest for NAA (1.04 mg L(-)(1) or 0.014 mg kg(-)(1)). The method has been applied for tomato samples fortified with the five phytohormones using a liquid-liquid extraction procedure, obtaining recovery percentages ranging from 91 to 109.0%.
Segura Carretero
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Department of Analytical Chemistry, Faculty of Science, University of Granada, Granada, Spain. ansegura@urg.es
Cruces-Blanco
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Soriano Peña
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Cortacero Ramírez
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Fernández Gutiérrez
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D000383:Agriculture; D019075:Electrophoresis, Capillary; D006863:Hydrogen-Ion Concentration; D018551:Solanum lycopersicum / Q000737:chemistry; D010937:Plant Growth Regulators / Q000032:analysis*; D012680:Sensitivity and Specificity
D010937:Plant Growth Regulators