After facial nerve damage, regenerating axons become aberrant throughout the length of the nerve and not only at the site of the lesion: an experimental study.
After facial nerve trauma, aberrant regeneration is associated with synkinesis. Animal models of mechanical nerve guides or reparative cell transplants at the site of a lesion have not been shown to improve disorganized regeneration. We examined whether this is because regenerating axons become disorganized throughout the length of the nerve and not only at the site of the lesion. In rats (n = 12), retrograde fluorescent tracer techniques were used to establish that most of the temporal branch fibres were carried in the superior half of the facial nerve trunk. In two further groups of rats (n = 24) a complete proximal facial nerve lesion was made, and the nerve immediately repaired by suture. After 4 weeks, at a second operation, the superior half of the facial nerve trunk was cut, either proximal or distal to the original lesion, and retrograde tracers were applied to distal branches of the nerve. It was possible to localize the points at which regenerating fibres became aberrant in their course by studying the number of labelled motoneurons in the facial nucleus after application of the tracer to the temporal branch of the nerve: this was similar in the distal and proximal hemisection groups, suggesting that aberrant axonal development occurred throughout the length of the nerve. Future strategies aimed at improving the organization of regeneration need to provide guidance cues not only at the site of the lesion as previously thought, but also throughout the length of the nerve.
Choi
D
D
Division of Neurobiology, National Institute for Medical Research, London UK. dchoi@nimr.mrc.ac.uk
Raisman
G
G
D000578:Amidines; D000818:Animals; D001369:Axons / Q000502:physiology*; D020220:Facial Nerve Injuries / Q000503:physiopathology*; D005260:Female; D005456:Fluorescent Dyes; D009046:Motor Neurons / Q000502:physiology; D009416:Nerve Regeneration / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains
D000578:Amidines; D005456:Fluorescent Dyes; C031455:diamidino compound 253-50