title: Expression of alphav, alpha4, alpha5 and beta3 integrin subunits, fibronectin and vitronectin in goat peri-implantation.
abstract: Integrins are glycoprotein heterodimers located in the cell membranes that stimulate intercellular adhesion and act as extracellular matrix (ECM) protein receptors. Although integrins have been detected in the implantation sites of various species, little is known about their participation in ruminant non-invasive placentation. The objective of this study was the detection of alphav, alpha4, alpha5, beta1 and beta3 integrin subunits and of two of their ligands, fibronectin and vitronectin, to determine their participation in the caprine peri-implantation process. On Day 21 post-coitum (pc), endometrial epithelium and trophoblastic cells showed an intense alphav and beta3 integrin subunits expression and moderate staining for alpha4 and alpha5. On Day 23 pc, integrin expression decreased noticeably and only a weak staining of alpha4 and beta3 integrin subunits were observed. No beta1 integrin subunit expression was detected on either of the days studied. Fibronectin (FN) expression in trophectodermic and endometrial epithelium was weak or moderate on the days studied while vitronectin (VN) expression in the same tissues was moderate or strong on Day 21 pc but decreased on Day 23 pc. These results suggest that alphavbeta3 integrin, alpha4 and alpha5 subunits, VN and FN are expressed in caprine endometrium and blastocyst and may play a role in the cascade of the implantation process.
authors.affiliation: Departamento de Patología Animal II, Facultad de Veterinaria, Universidad Complutense de Madrid, Ciudad Universitaria, E-28040 Madrid, Spain.
authors.forename: P
authors.initials: P
authors.lastname: García
authors.forename: A
authors.initials: A
authors.lastname: Nieto
authors.forename: M A
authors.initials: MA
authors.lastname: Sánchez
authors.forename: M
authors.initials: M
authors.lastname: Pizarro
authors.forename: J M
authors.initials: JM
authors.lastname: Flores
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000737:chemistry; D010064:Embryo Implantation; D004717:Endometrium / Q000737:chemistry*; D004847:Epithelial Cells / Q000737:chemistry; D005260:Female; D005353:Fibronectins / Q000032:analysis*; D019084:Fluorescent Antibody Technique, Indirect; D005865:Gestational Age; D006041:Goats / Q000378:metabolism*; D007150:Immunohistochemistry; D039441:Integrin alpha4 / Q000032:analysis; D039482:Integrin alpha5 / Q000032:analysis; D039564:Integrin alphaV / Q000032:analysis; D039661:Integrin beta3 / Q000032:analysis; D016023:Integrins / Q000032:analysis*; D011247:Pregnancy; D017154:Stromal Cells / Q000737:chemistry; D014327:Trophoblasts / Q000737:chemistry*; D019096:Vitronectin / Q000032:analysis*
chemical_list: D005353:Fibronectins; D039482:Integrin alpha5; D039564:Integrin alphaV; D039661:Integrin beta3; D016023:Integrins; D019096:Vitronectin; D039441:Integrin alpha4