title: [Experimental studies on the apoptosis of HL-60 cells induced by Brucea javanica oil emulsion].
abstract: OBJECTIVE
  To investigate the inducing effect of Brucea javanica on the apoptpsis of HL-60 cells.
  
  
  METHODS
  HL-60 cells were treated with Brucea javanica 1:100, 1:40, 1:20 (v/v) respectively for 6 h and DNA agarose gel electrophoresis, flow cytometry, fluorescence microscope, electron microscope were used to observe the apoptosis inducing effect of Brucea javanica.
  
  
  RESULTS
  DNA ladder was seen in the 1:40 group. The apoptosis cell percentages of 1:40 and 1:20 group were 86.8% and 97% respectively. Cells of 1:40 group showed obvious apoptosis character under fluorescence microscope. Cells were induced apoptosis in 1:20 and 1:40 Brucea javanica under electron microscope.
  
  
  CONCLUSIONS
  1:20 and 1:40 Brucea Javanica showed obvious apoptosis inducing effect of HL-60.
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China. Wangfangpumc@hotmail.com
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Cao
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Liu
authors.forename: Zhao-di
authors.initials: ZD
authors.lastname: Fu
authors.forename: Rui
authors.initials: R
authors.lastname: Han
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D032269:Brucea / Q000737:chemistry; D018922:HL-60 Cells; D006801:Humans; D010938:Plant Oils / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010938:Plant Oils