Formation of LacNAc mimetics employing novel donor substrates for enzymatic beta 1-->4 galactosylation.
In examining C-6 modified 4-nitrophenyl beta-D-galactopyranosides as donor structures the beta-galactosidase (Bacillus circulans) revealed an unexpectedly broad substrate specificity which allowed successful syntheses of various disaccharide components.
Weingarten
Saskia
S
Institute of Organic Chemistry, Hamburg University, Martin-Luther-King-Platz 6, D-20146, Hamburg, Germany.
Thiem
Joachim
J
D000606:Amino Sugars / Q000737:chemistry*; D001407:Bacillus / Q000201:enzymology*; D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000138:chemical synthesis*; D005690:Galactose / Q000031:analogs & derivatives / Q000737:chemistry; D008969:Molecular Sequence Data; D013379:Substrate Specificity; D001616:beta-Galactosidase / Q000737:chemistry*
D000606:Amino Sugars; D004187:Disaccharides; C000458:N-acetyllactosamine; D001616:beta-Galactosidase; D005690:Galactose