Production of neuroprotective NGF in astrocyte-T helper cell cocultures is upregulated following antigen recognition.
Astrocytic production of nerve growth factor (NGF) is increased during inflammation of the central nervous system (CNS). Here we show that cell-cell interaction between primary murine astrocytes and myelin basic protein (MBP)-specific T cell receptor (TCR) transgenic Th1 and Th2 cells significantly increased production of NGF. This upregulation was found to be dependent on antigen recognition. Neutralization of cytokines produced in cocultures did not affect NGF production. This novel finding suggests a neuroprotective role of astrocytes during T cell-mediated inflammation in the CNS.
Øren
Anita
A
Max Delbrück Center for Molecular Medicine, Robert-Rössle-Strasse 10, Berlin D-13125, Germany.
Falk
Kirsten
K
Rötzschke
Olaf
O
Bechmann
Ingo
I
Nitsch
Robert
R
Gimsa
Ulrike
U
D000818:Animals; D000906:Antibodies / Q000378:metabolism; D001253:Astrocytes / Q000378:metabolism*; D039481:CD11b Antigen / Q000378:metabolism; D002450:Cell Communication / Q000502:physiology; D002452:Cell Count / Q000379:methods; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D018920:Coculture Techniques / Q000379:methods; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D004676:Myelin Basic Protein / Q000276:immunology*; D020932:Nerve Growth Factor / Q000378:metabolism*; D010047:Ovalbumin / Q000276:immunology / Q000378:metabolism; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism*; D015854:Up-Regulation / Q000276:immunology; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
D000906:Antibodies; D039481:CD11b Antigen; D016207:Cytokines; D000949:Histocompatibility Antigens Class II; D004676:Myelin Basic Protein; C111390:OVA 323-339; D010446:Peptide Fragments; D011948:Receptors, Antigen, T-Cell; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D010047:Ovalbumin; D020932:Nerve Growth Factor