[A comparison of the protective activity of liver injury induced by D-galact-osamine between huangqin-tang and their metabolites by human intestinal bacteria].
OBJECTIVE
To compare the protective activity of liver injury induced by D-galactosamine (GalN) between Huangqin-Tang and their metabolites by human intestinal bacteria(HIB).


METHODS
The liver injuries in conventional and pseudo-germfree mice were induced by GalN. After oral administration of Huangqin-Tang and their metabolites mixtures by HIB, the serum transaminase (ALT and AST) activities were detected.


RESULTS
In conventional mice, large and medium doses (20 and 10 g.kg-1) of Huangqin-Tang decoction significantly reduced the increase of serum ALT activity after 18 h GalN treatment. In pseudo-germfree mice, metabolites significantly reduced the ALT levels. However, Huangqing-Tang didn't affect the ALT levels in this kind of mice. To all of the animals, AST levels remained the same after oral Huangqin-tang or their metabolites.


CONCLUSIONS
The metabolism by intestinal bacteria plays a role in pharmacological effects of constituents of Chinese herbal medicine. The metabolites of the constituents by intestinal bacteria were the real active components in vivo.
Zuo
Feng
F
Institute of Chinese Material Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Zhou
Zhong-ming
ZM
Yan
Mei-zhen
MZ
Xiong
Yu-lan
YL
Wan
Yan-li
YL
Song
Hong-yue
HY
Wu
Zi-lun
ZL
He
Rong
R
Hui
Lian-qiang
LQ
Ye
Wen-hua
WH
D000284:Administration, Oral; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001419:Bacteria / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D005688:Galactosamine; D007422:Intestines / Q000382:microbiology*; D008107:Liver Diseases / Q000378:metabolism*; D008297:Male; D051379:Mice; D010946:Plants, Medicinal / Q000737:chemistry; D020011:Protective Agents / Q000378:metabolism / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; C439205:Huangqin-Tang; D020011:Protective Agents; D005688:Galactosamine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase