title: Levels of expression of CYR61 and CTGF are prognostic for tumor progression and survival of individuals with gliomas.
abstract: The biological properties of CCN proteins include stimulation of cell proliferation, migration, and adhesion, as well as angiogenesis and tumorigenesis. We quantified CYR61, CTGF, WISP-1, and NOV mRNA expression levels in samples from sixty-six primary gliomas and five normal brain samples using quantitative real-time PCR assay. Statistical analysis was performed to explore the links between expression of the CCN genes and clinical and pathological parameters. Overexpression of CYR61, CTGF, WISP-1, and NOV occurred in 48% (32 of 66), 58% (38 of 66), 36% (24 of 66), and 15% (10 of 66) of primary gliomas, respectively. Interestingly, significant associations were found between CYR61 expression versus tumor grade, pathology, gender, and age at diagnosis. Also, a significant correlation existed between CTGF mRNA levels versus tumor grade, gender, and pathology. In contrast to CYR61 and CTGF, no significant association was found between expression of either WISP-1 or NOV versus any of the pathological features. Furthermore, Cox regression analysis showed that CYR61 and CTGF expression had a significant correlation with patient survival. These results suggest that CYR61 and CTGF may play a role in the progression of gliomas; their levels at diagnosis may have prognostic significance; and these proteins might serve as valuable targets for therapeutic intervention.
authors.affiliation: Division of Hematology/Oncology, Cedars-Sinai Medical Center, University of California-Los Angeles (UCLA) School of Medicine, Los Angeles, California 90048, USA. xied@ucla.edu
authors.forename: Dong
authors.initials: D
authors.lastname: Xie
authors.forename: Dong
authors.initials: D
authors.lastname: Yin
authors.forename: He-Jing
authors.initials: HJ
authors.lastname: Wang
authors.forename: Gen-Tao
authors.initials: GT
authors.lastname: Liu
authors.forename: Robert
authors.initials: R
authors.lastname: Elashoff
authors.forename: Keith
authors.initials: K
authors.lastname: Black
authors.forename: H Phillip
authors.initials: HP
authors.lastname: Koeffler
mesh_terms: D000328:Adult; D001932:Brain Neoplasms / Q000235:genetics / Q000401:mortality / Q000601:surgery; D055513:Connective Tissue Growth Factor; D055515:Cysteine-Rich Protein 61; D018450:Disease Progression; D005260:Female; D005910:Glioma / Q000235:genetics* / Q000401:mortality / Q000601:surgery; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D055514:Nephroblastoma Overexpressed Protein; D011379:Prognosis; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016019:Survival Analysis; D013997:Time Factors
chemical_list: C501848:CCN1 protein, human; C526317:CCN2 protein, human; C526319:CCN3 protein, human; D055515:Cysteine-Rich Protein 61; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D055514:Nephroblastoma Overexpressed Protein; D055513:Connective Tissue Growth Factor