title: Observation on in situ hybridization and immunocytochemistry of matrix metalloproteinases in rat pancreas.
abstract: In situ hybridization and immunocytochemical techniques were employed to examine the expression of matrix metalloproteinases-1 (MMP-1) and to identify the pattern of its distribution in rat pancreas. The results indicated that the signal of MMP-1 mRNA and MMP-1 positive immunoreaction were detected in some fiberoblasts around interlobular ducts and exocrine cell in margin acinus of some lobules, but the signal of MMP-1 mRNA and MMP-1 positive immunoreaction could not be detected in most of other acinus and islets of pancreas. It is concluded that the expression of MMP-1 in above cells of rat might play an important role in acinar proliferation and differentiation of rat pancreatic tissues.
authors.affiliation: Department of Histology and Embryology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
authors.forename: Lihua
authors.initials: L
authors.lastname: Tang
authors.forename: Shenghong
authors.initials: S
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Zilong
authors.initials: Z
authors.lastname: Liu
authors.forename: Yun
authors.initials: Y
authors.lastname: Xu
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.forename: Zhaochun
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D002455:Cell Division; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D010179:Pancreas / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D012333:RNA, Messenger; D020781:Matrix Metalloproteinase 1