title: Evidences for the formation of bisbenzamidine-heme complexes in cell-free systems.
abstract: Infrared and colorimetry data suggest that bisbenzamidines connected by various rigid or flexible linkers are able to interact with heme in cell-free systems. At pH 5.0 the inhibition of formation of beta-hematin could be ascertained by infrared spectroscopy whereas at pH 7.0 the interaction yielded insoluble complexes for which a sandwich-type structure of stoichiometry 2:1, heme-drug, is tentatively proposed.
authors.affiliation: Xavier University of Louisiana, College of Pharmacy, Division of Basic Pharmaceutical Sciences, 1 Drexel Drive, New Orleans, LA 70125, USA.
authors.forename: Annie
authors.initials: A
authors.lastname: Mayence
authors.forename: Jean Jacques
authors.initials: JJ
authors.lastname: Vanden Eynde
authors.forename: Tien L
authors.initials: TL
authors.lastname: Huang
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D001550:Benzamidines / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D002474:Cell-Free System; D003124:Colorimetry / Q000379:methods; D006418:Heme / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D010963:Plasmodium falciparum / Q000187:drug effects / Q000378:metabolism
chemical_list: D000962:Antimalarials; D001550:Benzamidines; D006418:Heme