Morphology-associated expression of NADP-dependent glutamate dehydrogenases during yeast-mycelium transition of a dimorphic fungus Benjaminiella poitrasii.
Benjaminiella poitrasii, a dimorphic zygomycetous fungus possesses three glutamate dehydrogenases, one requiring NAD while the other two use NADP as a coenzyme. In the activity staining after electrophoresis on native polyacrylamide gel NAD- dependent glutamate dehydrogenase revealed the presence of one enzyme that was expressed in both, yeast- and mycelium-form cells. While in case of NADP- dependent glutamate dehydrogenase two distinct activity bands that were differentially expressed in yeast- and mycelium-form cells were seen. Interestingly, during yeast-mycelium transition and reverse, quantitative changes in form-specific native NADP-dependent glutamate dehydrogenase activities were seen. The biochemical data on temperature and pH optima, thermostability, and kinetic properties confirmed the presence of two NADP-dependent proteins in B. poitrasii, parent strain. The monomorphic mutant (Y-5, yeast form) showed NADP- glutamate dehydrogenase similar to parent yeast-form enzyme. For the first time the significance of differential expression of these enzymes during morphological transition in B. poitrasii has been suggested.
Amin
Aradhana
A
Biochemical Sciences Division, National Chemical Laboratory, Pune-411008 India.
Joshi
Mangesh
M
Deshpande
Mukund V
MV
D004795:Enzyme Stability; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D018086:Glutamate Dehydrogenase (NADP+) / Q000235:genetics / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009090:Mucorales / Q000166:cytology* / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D025282:Mycelium / Q000166:cytology / Q000502:physiology*; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010766:Phosphorylation; D013696:Temperature
D005656:Fungal Proteins; D009243:NAD; D009249:NADP; D018086:Glutamate Dehydrogenase (NADP+)