Human cystic echinococcosis: evaluation of post-treatment serologic follow-up by IgG subclass antibody detection.
Assessment of post-treatment disease activity among patients with cystic echinococcosis (CE) is insensitive using detection of CE-specific total IgG antibody. This study investigated whether serum concentrations of CE-specific IgG subclasses 1-4 correlate better with disease activity than total IgG. We studied a cohort of patients (n = 28) with symptomatic CE treated with anthelminthic drugs and surgery and who were followed up clinically and radiologically for a mean of 5.6 years (range = 3-12 years). Serial archived sera collected during follow-up were retrospectively analyzed by enzyme-linked immunosorbent assays using crude horse hydatid cyst fluid as antigen. Changes in concentrations of antibodies were correlated with clinical and radiologic outcome. At diagnosis, concentrations of CE-specific total IgG, IgG1, and IgG2 antibodies were significantly elevated in a greater proportion of patients compared with IgG3 and IgG4 antibodies. During post-treatment follow up, the IgG2 antibody response provided the best correlate of disease activity.
Lawn
Stephen D
SD
Hospital for Tropical Diseases, London, United Kingdom. stevelawn@yahoo.co.uk
Bligh
John
J
Craig
Philip S
PS
Chiodini
Peter L
PL
D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D015153:Blotting, Western; D004443:Echinococcosis / Q000175:diagnosis / Q000188:drug therapy*; D004446:Echinococcus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000145:classification*; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies
D000909:Antibodies, Helminth; D007074:Immunoglobulin G