title: A patch-clamp investigation of membrane currents in a novel mammalian retinal ganglion cell line.
abstract: We characterised membrane currents in undifferentiated RGC-5 cells, a cell line used in in vitro models of apoptosis and glaucoma. The cells were inexcitable, with no voltage-dependent Na(+) currents or action potentials. Some novel currents were observed including basal Cl(-) currents, inwardly rectifiying K(+) currents and Gd(3+) insensitive stretch-activated currents. Our results highlight the differences between the electrophysiological properties of undifferentiated RGC-5 cells and retinal ganglion cells.
authors.affiliation: School of Medical Sciences, The University of New South Wales, Sydney, 2052, Australia.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Moorhouse
authors.forename: Shaojuan
authors.initials: S
authors.lastname: Li
authors.forename: Richard M
authors.initials: RM
authors.lastname: Vickery
authors.forename: Mark A
authors.initials: MA
authors.lastname: Hill
authors.forename: John W
authors.initials: JW
authors.lastname: Morley
mesh_terms: D000818:Animals; D002460:Cell Line; D007473:Ion Channels / Q000502:physiology; D008564:Membrane Potentials / Q000502:physiology; D018408:Patch-Clamp Techniques / Q000379:methods; D012165:Retinal Ganglion Cells / Q000166:cytology* / Q000502:physiology*
chemical_list: D007473:Ion Channels