On-capillary complexation of metal ions with 4-(2-thiazolylazo)resorcinol in capillary electrophoresis.
Co(II), Zn(II), Ni(II) and Fe(I) were successfully separated by capillary electrophoresis using pre-capillary and on-capillary complexation with 4-(2-thiazolylazo)resorcinol. The influences of some crucial parameters, including both pre- and on-capillary complexation procedure, were investigated. For on-capillary complexation, the complexing reaction was carried out inside the capillary by mixing the zones of ligand and sample during the electrophoretic migration. Compared with pre-capillary complexation, the method provided 30-fold reduction in detection limits for Co(II), 50-fold reduction for Zn(II), and 100-fold reduction for Ni(II) and Fe(II). It was used for the analysis of a pharmaceutical and tap water sample.
Wang
Min
M
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, China.
Lin
Jin-Ming
JM
Qu
Feng
F
Shan
Xiaoquan
X
Chen
Zuliang
Z
D001391:Azo Compounds / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008670:Metals / Q000737:chemistry*; D012118:Resorcinols / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D001391:Azo Compounds; D008670:Metals; D012118:Resorcinols; C026054:4-(2-thiazolylazo)resorcinol