title: Approaches to identify, clone, and express symbiont bioactive metabolite genes.
abstract: This review discusses approaches to identify, clone, and express bioactive metabolite genes from symbionts of marine invertebrates. Criteria for proving symbiotic origin of bioactive metabolites are presented, followed by a comprehensive, practically-oriented overview of techniques to be applied. The Bugula neritina/Endobugula sertula association is used as a primary example, but other symbioses are discussed. Thirty-six compounds are presented and 111 references are cited.
authors.affiliation: Scripps Institution of Oceanography, Marine Biology Research Division; Center for Marine Biotechnology and Biomedicine; and UCSD Cancer Center, University of California, San Diego, La Jolla, California 92093, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Hildebrand
authors.forename: Laura E
authors.initials: LE
authors.lastname: Waggoner
authors.forename: Grace E
authors.initials: GE
authors.lastname: Lim
authors.forename: Katherine H
authors.initials: KH
authors.lastname: Sharp
authors.forename: Christian P
authors.initials: CP
authors.lastname: Ridley
authors.forename: Margo G
authors.initials: MG
authors.lastname: Haygood
mesh_terms: D000818:Animals; D001685:Biological Factors / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D005827:Genetics, Microbial; D007448:Invertebrates / Q000737:chemistry / Q000235:genetics; D008386:Marine Biology; D008954:Models, Biological; D015394:Molecular Structure; D017422:Sequence Analysis, DNA; D013559:Symbiosis / Q000235:genetics
chemical_list: D001685:Biological Factors