Allyl-isothiocyanate causes mitotic block, loss of cell adhesion and disrupted cytoskeletal structure in HT29 cells.
Epidemiological evidence indicates that Brassica vegetables protect against colorectal cancer. Brassicas contain glucosinolates, the breakdown products of which exert antiproliferative effects against cancer cells. We have examined the effects of allyl-isothiocyanate (AITC), a major breakdown product of the glucosinolate sinigrin, on proliferation and death of colorectal cancer cells. HT-29 colorectal cells were exposed to AITC for 24 h and the number of adherent and detached cells determined. Both populations were analysed for cell-cycle characteristics and examined by light and electron microscopy for features of apoptosis and mitosis. Evidence of apoptosis was also determined by flow cytometric analysis of Annexin V staining in the detached population of cells. AITC-treated cells were also stained for alpha-tubulin. Treatment caused cells to round up after 7 h of exposure and subsequently detach. At 24 h these cells were blocked in mitosis. Detached AITC-treated cells showed no signs of apoptosis as assessed by morphological features or by Annexin V staining but they did show evidence of disrupted tubulin. AITC inhibits proliferation of cancer cells by causing mitotic block associated with disruption of alpha-tubulin in a manner analogous to a number of chemotherapeutic agents.
Smith
Tracy K
TK
Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK.
Lund
Elizabeth K
EK
Parker
Mary L
ML
Clarke
Rosemary G
RG
Johnson
Ian T
IT
D017304:Annexin A5 / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis; D002448:Cell Adhesion / Q000187:drug effects; D002453:Cell Cycle; D002455:Cell Division; D045744:Cell Line, Tumor; D004396:Coloring Agents / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D005434:Flow Cytometry; D005520:Food Preservatives / Q000494:pharmacology*; D016193:G1 Phase; D006801:Humans; D017879:Isothiocyanates / Q000494:pharmacology*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008938:Mitosis; D010718:Phosphatidylserines / Q000737:chemistry; D016192:Resting Phase, Cell Cycle; D013997:Time Factors; D014404:Tubulin / Q000378:metabolism; D014675:Vegetables
D017304:Annexin A5; D000970:Antineoplastic Agents; D004396:Coloring Agents; D005520:Food Preservatives; D017879:Isothiocyanates; D010718:Phosphatidylserines; D014404:Tubulin; C004471:allyl isothiocyanate