[Expression of matrixmetalloproteinase-8 on the bell-stage in human and rat tooth development].
OBJECTIVE
To study the expression of MMP-8 in human and rat tooth development.


METHODS
Immunohistochemistry was used to detect the localization of MMP-8 protein while in situ hybridization was used to examine the expression of MMP-8 mRNA.


RESULTS
The expression of MMP-8 protein was localized in odontoblast and dentin matrix at the later bell stage in human tooth germ. The dentin was denser close to the pulp cavity. The expression of MMP-8 mRNA was found in very few polarized odontoblast at the early bell stage and all polarized odontoblast at the later bell stage in rat tooth germ.


CONCLUSIONS
The results suggested that MMP-8 involved in dentin matrix rebuilding in the process of dentin formation in human and rat dental development.
Hao
Yu-qing
YQ
Key Lab. of Oral Biomedical Engineering Ministy of Education, Sichuan University, Chengdu 610041, China.
Niu
Zhong-ying
ZY
Wang
Gou-quan
GQ
Zhou
Xue-dong
XD
Hu
Tao
T
D000818:Animals; D000831:Animals, Newborn; D003804:Dentin / Q000201:enzymology; D004622:Embryo, Mammalian; D017403:In Situ Hybridization; D020784:Matrix Metalloproteinase 8 / Q000096:biosynthesis* / Q000235:genetics; D008437:Maxilla / Q000201:enzymology; D009805:Odontogenesis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D014083:Tooth Germ / Q000196:embryology* / Q000201:enzymology
D012333:RNA, Messenger; D020784:Matrix Metalloproteinase 8