The effects of Ligustrazine on the expression of bFGF and bFGFR in bone marrow in radiation injured mice.
To study the expression of the bFGF and its receptor in the mouse bone marrow by treatment with acute radioactive injury and Ligustrazine, 56 mice were divided into 3 groups: normal group, radiation-injured group and Ligustrazine group. After irradiation by 6.0 Gy 60Co gamma-ray, each mouse was orally given 0.1 ml Ligustrazine twice a day for 13 days in Ligustrazine group, and each mouse in radiation injured group was orally given equal amount of saline. On the 3rd, 7th, 14th day after irradiation, bone marrow mono-nuclear cells (BMMNC) were counted, and the expression levels of bPGF and bFGFR in bone marrow were evaluated by immunohistochemistry and flow cytometry analysis respectively. On the 3rd, 7th, 14th day after irradiation, expression of bFGF in bone marrow were significantly lower than in normal group (P<0.05 or P<0.01). Expressions of bFGF and bFGFR were much higher in Ligustrazine treated group than that in the control group (P<0.05 or P<0.01). Ligustrazine potentiate the expression of bFGF and bFGFR in bone marrow MNC to recover the bone marrow hematopoiesis inductive microenvironment, which is one of the mechanisms by which Ligustrazine rebuild the bone marrow hematopoiesis after acute radioactive injury.
Wu
Ning
N
Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
Sun
Hanying
H
Liu
Wenli
W
Xu
Huizhen
H
Lu
Wu
W
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis*; D006410:Hematopoiesis / Q000187:drug effects; D008297:Male; D051379:Mice; D011719:Pyrazines / Q000494:pharmacology*; D011833:Radiation Injuries, Experimental / Q000378:metabolism*; D011837:Radiation-Protective Agents / Q000494:pharmacology; D017468:Receptors, Fibroblast Growth Factor / Q000096:biosynthesis*
D011719:Pyrazines; D011837:Radiation-Protective Agents; D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2; C017953:tetramethylpyrazine