Localization of arginine decarboxylase in tobacco plants.
The lack of knowledge about the tissue and subcellular distribution of polyamines (PAs) and the enzymes involved in their metabolism remains one of the main obstacles in our understanding of the biological role of PAs in plants. Arginine decarboxylase (ADC; EC 4.1.1.9) is a key enzyme in polyamine biosynthesis in plants. We have characterized a cDNA coding for ADC from Nicotiana tabacum L. cv. Petit Havana SR1. The deduced ADC polypeptide had 721 amino acids and a molecular mass of 77 kDa. The ADC cDNA was overexpressed in Escherichia coli, and the ADC fusion protein obtained was used to produce polyclonal antibodies. Using immunological methods, we demonstrate the presence of the ADC protein in all plant organs analysed: flowers, seeds, stems, leaves and roots. Moreover, depending on the tissue, the protein is localized in two different subcellular compartments, the nucleus and the chloroplast. In photosynthetic tissues, ADC is located mainly in chloroplasts, whereas in non-photosynthetic tissues the protein appears to be located in nuclei. The different compartmentation of ADC may be related to distinct functions of the protein in different cell types.
Bortolotti
Cristina
C
Universitat de Barcelona. Facultat de Farmacia, Unitat de Fisiologia Vegetal, E-08028 Barcelona, Spain Instituto de Biologia Molecular y Celular de Plantas (IBMCP), E-46022 Valencia, Spain.
Cordeiro
Alexandra
A
Alcázar
Rubén
R
Borrell
Antoni
A
Culiañez-Macià
Francisco A.
FA
Tiburcio
Antonio F.
AF
Altabella
Teresa
T