title: 3D reconstruction of the hemocyanin subunit dimer from the chiton Acanthochiton fascicularis.
abstract: Procedures are presented for the purification of the subunit dimer from Acanthochiton fasicularis hemocyanin. Electron microscopy of negatively stained specimens revealed a uniform population of macromolecules possessing the characteristic "boat shape". A 3D reconstruction from this EM data generated a approximately 3 nm resolution model that correlates well with earlier data of the purported subunit dimer, extracted from the 3D reconstruction of the didecamer of Haliotis tuberculata hemocyanin type 1.
authors.affiliation: Institute of Zoology, Johannes Gutenberg University, D-55099, Mainz, Germany.
authors.forename: J Robin
authors.initials: JR
authors.lastname: Harris
authors.forename: Ulrich
authors.initials: U
authors.lastname: Meissner
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Gebauer
authors.forename: Jürgen
authors.initials: J
authors.lastname: Markl
mesh_terms: D000818:Animals; D019281:Dimerization; D006433:Hemocyanins / Q000737:chemistry*; D008854:Microscopy, Electron; D008974:Mollusca / Q000737:chemistry*; D021122:Protein Subunits
chemical_list: D021122:Protein Subunits; D006433:Hemocyanins