title: SKN7 of Candida albicans: mutant construction and phenotype analysis.
abstract: The SKN7 two-component response regulator gene of Candida albicans was deleted, and the phenotype of the mutant was established. This mutant exhibited impaired growth on Spider agar and 10% serum agar compared to wild-type and gene-reconstituted strains. The skn7 mutant was sensitive to H(2)O(2) in vitro, but its virulence was only mildly attenuated. A comparison of the Skn7p and Ssk1p response regulators of C. albicans is discussed.
authors.affiliation: Department of Microbiology and Immunology, Georgetown University Medical Center, Washington, DC 20007, USA.
authors.forename: Praveen
authors.initials: P
authors.lastname: Singh
authors.forename: Neeraj
authors.initials: N
authors.lastname: Chauhan
authors.forename: Anup
authors.initials: A
authors.lastname: Ghosh
authors.forename: Freddie
authors.initials: F
authors.lastname: Dixon
authors.forename: Richard
authors.initials: R
authors.lastname: Calderone
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity* / Q000502:physiology*; D002177:Candidiasis / Q000382:microbiology / Q000503:physiopathology*; D003470:Culture Media; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004195:Disease Models, Animal; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D051379:Mice; D018384:Oxidative Stress; D010641:Phenotype; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014774:Virulence
chemical_list: D003470:Culture Media; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C083962:SKN7 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D006861:Hydrogen Peroxide