title: [Studies on callus induced from leaves and plantlets regeneration of the traditional Chinese medicine Chrysanthemum morifolium].
abstract: OBJECTIVE
  To set up the optimums for the leaf tissue culture of Chrysanthemum morifolium cultivated in Anhui Province.
  
  
  METHODS
  Leaves were cut and cultured onto the same kind of media on different sides and defferent kinds of media on the same side, and induced to form the whole plantlet.
  
  
  CONCLUSIONS
  All the media used could induce callus, while the results of callus redifferentiation were very distinct. The media with NAA 0.1 mg.L-1 + 6-BA 0.1-1.0 mg.L-1 were much better than others when callus were induced. The MS medium with 6-BA 2.0 mg.L-1 and NAA 0.5 mg.L-1 was the preferable medium for the bud sprouting. The regenerated plantlets produced variation of leaf shape.
authors.affiliation: Department of Biology, Huaibei Coal Industry Teacher's College, Huabei 235000, Anhui, China. xueJP2000@yahoo.com.cn
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Xue
authors.forename: Miao
authors.initials: M
authors.lastname: Yu
authors.forename: Ai-min
authors.initials: AM
authors.lastname: Zhang
mesh_terms: D031202:Chrysanthemum / Q000254:growth & development*; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D018515:Plant Leaves / Q000254:growth & development; D010946:Plants, Medicinal / Q000254:growth & development*
chemical_list: D003470:Culture Media