cDNA cloning and molecular characterization of a cysteine-rich gene from Campoletis chlorideae polydnavirus.
Polydnavirus (PDV) of Campoletis chlorideae (CcIV) is very important for the successful development of the parasitoid progenies. Previous study revealed that the persistence and expression of CcIV in parasitized Helicoverpa armigera larvae continued for 5 days, and the 1.0 kb gene (CcIV 1.0) was most abundantly expressed. In this report, a cDNA library was constructed using the SMART cDNA Synthesis Method, and the CcIV 1.0 was cloned and identified by PCR, Southern blot hybridization and 5' end amplification, this gene is 936 bp long and encodes 207 amino acids with a signal peptide and a cysteine motif. Sequence comparison shows CcIV 1.0 has high identity with VHv 1.4, VHv 1.1 genes (86%, 88%) and WHv 1.6, WHv 1.0 genes (89%, 87%) of Campoletis sonorensis PDV, which might suggest that they have arisen from a common ancestral gene; the homology between CcIV and other PDV genes is not significant.
Zhang
Cong
C
State Key Laboratory of Integrated Management of Pest Insects & Rodents, Institute of Zoology, Chinese Academy of Sciences, 25 Beisihuan Xilu, Beijing 100080, China.
Wang
Chen-Zhu
CZ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003545:Cysteine / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D005814:Genes, Viral; D007814:Larva; D008969:Molecular Sequence Data; D018071:Polydnaviridae / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014863:Wasps / Q000821:virology*
D018076:DNA, Complementary; D003545:Cysteine