Determination of the effective dose of an experimental fasciolicide in naturally and experimentally infected cattle.
The aim of the present study was to determine the effective dose of an experimental fasciolicide called compound alpha or 5-chloro-2-methylthio-6-(1-napthyloxy)1H-benzimidazole in experimentally and naturally infected cattle. In the first experiment, 24 fluke-free heifers were each infected with 800 metacercariae of Fasciola hepatica and re-infected on day 45 with other 600 cysts per animal. On day 75, when the animals had 4- and 10-week-old flukes respectively, they were divided into four groups (G) of six animals each according to fluke egg counts. Groups 1-3 received compound alpha at 10, 12 and 14 mg/kg/p.o., respectively. G4 remained as an untreated control. Twenty days after treatment, the animals were sacrificed for the recovery of flukes. Efficacy was assessed as a percentage of egg or fluke reduction relative to the untreated control. In the second experiment (naturally infected cattle), 24-year-old steers positive to F. hepatica eggs were blocked into four groups of five animals each. Groups 1-3 received compound alpha at 10, 12 and 14 mg/kg/p.o., respectively. Group 4 served as a non-treated control. All procedures to determine efficacy were carried out as mentioned in the first experiment. The results in the first study showed a percentage on egg reduction of 97.3, 100 and 100 and overall fluke reduction of 94.3, 100 and 100 for Groups 1-3, respectively. In the second study, the percentage of egg reduction was of 87.5, 99.1 and 100 and overall efficacy regarding fluke reduction was of 84.2, 99.6, and 100 for Groups 1-3, respectively. It is concluded that the effective dose selected for compound alpha was of 12 mg/kg/p.o. in cattle having an induced or natural F. hepatica infection.
Ibarra
Froylán
F
Departamento de Parasitología, Fac. de Med. Vet. y Zoot., UNAM. Cd. Universitaria, 04510 Mexico, DF, Mexico. ibarraf@servidor.unam.mx
Vera
Yolanda
Y
Quiroz
Héctor
H
Cantó
Jorge
J
Castillo
Rafael
R
Hernández
Alicia
A
Ochoa
Pedro
P
D000284:Administration, Oral; D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage*; D000909:Antibodies, Helminth / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy* / Q000469:parasitology*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005210:Fasciola hepatica / Q000254:growth & development* / Q000378:metabolism; D005211:Fascioliasis / Q000188:drug therapy* / Q000469:parasitology / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D005260:Female; D007093:Imidazoles / Q000008:administration & dosage*; D008099:Liver / Q000469:parasitology; D008297:Male; D009281:Naphthalenes / Q000008:administration & dosage*; D010270:Parasite Egg Count / Q000662:veterinary; D018709:Statistics, Nonparametric
C468032:5-chloro-2-methylthio-6-(1-naphthyloxy)-1H-benzimidazole; D000871:Anthelmintics; D000909:Antibodies, Helminth; D007093:Imidazoles; D009281:Naphthalenes