title: Apoptotic mitochondrial dysfunction induced by benzo(a)pyrene in liver epithelial cells: role of p53 and pHi changes.
abstract: How pH(i) changes, more specifically alkalinization, affect the apoptotic cascade has yet to be determined. The aim of the present work was to test the involvement of mitochondria in the apoptotic cascade triggered by benzo(a)pyrene [B(a)P] and to determine the role of pH(i) changes and p53 relative to mitochondria. Our results indicate that B(a)P-induced apoptosis might rely upon a p53-dependent and a pH-sensitive mitochondrial dysfunction.
authors.affiliation: INSERM U456, Faculté de Pharmacie, Université Rennes I, 2 avenue Prof. Léon Bernard, 35043 Rennes cedex, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Huc
authors.forename: David
authors.initials: D
authors.lastname: Gilot
authors.forename: Claire
authors.initials: C
authors.lastname: Gardyn
authors.forename: Mary
authors.initials: M
authors.lastname: Rissel
authors.forename: Marie-Thérèse
authors.initials: MT
authors.lastname: Dimanche-Boitrel
authors.forename: André
authors.initials: A
authors.lastname: Guillouzo
authors.forename: Olivier
authors.initials: O
authors.lastname: Fardel
authors.forename: Dominique
authors.initials: D
authors.lastname: Lagadic-Gossmann
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D001564:Benzo(a)pyrene / Q000633:toxicity*; D052160:Benzothiazoles; D001865:Bongkrekic Acid / Q000494:pharmacology; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D006146:Guanidines / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure*; D051381:Rats; D013450:Sulfones / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D014050:Toluene / Q000031:analogs & derivatives* / Q000494:pharmacology; D016159:Tumor Suppressor Protein p53 / Q000187:drug effects
chemical_list: D052160:Benzothiazoles; D006146:Guanidines; D013450:Sulfones; D013844:Thiazoles; D016159:Tumor Suppressor Protein p53; D001865:Bongkrekic Acid; D001564:Benzo(a)pyrene; D014050:Toluene; C093373:cariporide; C121565:pifithrin