title: Molecular characterization of the integrons in Shigella strains isolated from patients with traveler's diarrhea.
abstract: The prevalence and characterization of Class 1 integrons has been performed in eighty three strains of Shigella spp., isolated between 1995 and 2000 from patients with traveler's diarrhea. A low prevalence (13.25%) was recorded. Nine different integrons were found among 11 multiresistant strains, with a total of 10 different gene cassettes encoding for resistance to trimethoprim (dfrA1, dfrA5, dfrA7, dfrA12, and dfrA15), aminoglycosides (aadA1a and aadA2), beta-lactam antibiotics (oxa2) or ORF with unknown function (orfD and orfF). A high prevalence of dfr and aad gene cassettes was observed. The low incidence of Class 1 integrons observed in this study is in contrast with the known facility that the Shigella genus has to gain and transfer plasmids.
authors.affiliation: Servei de Microbiologia, Institut Clínic Infeccions i Immunologia, Hospital Clínic-IDIBAPS, University of Barcelona, Villarroel, 170, 08036 Barcelona, Spain.
authors.forename: M M
authors.initials: MM
authors.lastname: Navia
authors.forename: J
authors.initials: J
authors.lastname: Ruiz
authors.forename: J
authors.initials: J
authors.lastname: Vila
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D024901:Drug Resistance, Multiple, Bacterial; D004405:Dysentery, Bacillary / Q000188:drug therapy / Q000453:epidemiology / Q000235:genetics*; D006801:Humans; D032023:Integrons / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008967:Molecular Biology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D012760:Shigella / Q000145:classification / Q000235:genetics*; D013030:Spain / Q000453:epidemiology; D014195:Travel
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial