Association between I(2) binding sites and monoamine oxidase-B activity in platelets.
An I(2) imidazoline binding site on monoamine oxidase-B (MAO-B) is known to be encoded by a noncatalytic part of the enzyme, different from that which recognizes mechanism-based inhibitors. Herein, the relationship between I(2)-imidazoline binding sites and MAO-B activity has been assessed using a semi-purified source of MAO-B: platelet mitochondrial membranes. A positive correlation between I(2) sites and MAO-B activity was observed (r = 0.61, P = 0.0016) among 24 human subjects. Nevertheless, the variance in MAO-B activity cannot be completely accounted for in relation to I(2) sites. Therefore, I(2) density and MAO-B activity are only weakly correlated in platelets.
Zhu
H
H
University of Mississippi Medical Center, Jackson, Mississippi 39216, USA. HZhu@psychiatry.umsmed.edu
Piletz
J E
JE
D000328:Adult; D001792:Blood Platelets / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D005260:Female; D006801:Humans; D007093:Imidazoles / Q000378:metabolism; D054755:Imidazoline Receptors; D007457:Iodine Radioisotopes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism; D008995:Monoamine Oxidase / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism*; D011955:Receptors, Drug / Q000378:metabolism*; D013223:Statistics as Topic
D007093:Imidazoles; D054755:Imidazoline Receptors; D007457:Iodine Radioisotopes; D020033:Protein Isoforms; D011955:Receptors, Drug; C096331:iodoazidophenoxymethylimidazoline; D008995:Monoamine Oxidase