title: Two-dimensional diversity: screening human cDNA phage display libraries with a random diversity probe for the display cloning of phosphotyrosine binding domains.
abstract: A random phosphopeptide probe (bio-pYZZZ) has been used for the isolation and identification of multiple SH2 domains from human cDNA-displaying phage libraries. In addition, on-phage analysis and quantification of binding affinities for these phage-displayed proteins has shown them to be functional domains, retaining the same characteristics as in their native state.
authors.affiliation: Department of Chemistry, Yale University, New Haven, Connecticut 06520, USA.
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Videlock
authors.forename: Victor K
authors.initials: VK
authors.lastname: Chung
authors.forename: Michael A
authors.initials: MA
authors.lastname: Mohan
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Strok
authors.forename: David J
authors.initials: DJ
authors.lastname: Austin
mesh_terms: D003001:Cloning, Molecular; D003090:Coliphages / Q000235:genetics; D015723:Gene Library; D006801:Humans; D008024:Ligands; D015394:Molecular Structure; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D015398:Signal Transduction; D018909:src Homology Domains
chemical_list: D008024:Ligands; D010446:Peptide Fragments; D019000:Phosphotyrosine