[Analysis of genomic polymorphism of typical and atypical strains of the plague pathogen using polymerase chain reaction with universal primers].
An analysis of genome polymorphism of the Y. pestis strains by using the method of polymerase chain reaction (PCR) for the tandem repeats of bacteriophage M13 DNA revealed a species similarity of both typical and atypical (according to diagnostic signs) plague-microbe strains. Strain Y. pestis A-1726 with the atypical differential-and-diagnostic properties, without the amplicon specified for Y. pestis and sized 1000 b.p., was identified among 27 analyzed Y. pestis strains. The amplicon profiles of the basic Y. pestis subtype were found to be different from such profiles of other Y. pestis subtypes.
Savostina
E P
EP
Microb Research Institute for Plague Control, Saratov, Russia.
Popov
Iu A
IuA
Kashmanova
T N
TN
Iashechkin
Iu I
IuI
D017104:Bacteriophage M13 / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D015010:Yersinia pestis / Q000235:genetics*
D017931:DNA Primers