Design, synthesis, and characterization of peptide nanostructures having ion channel activity.
We report the synthesis and the functional studies of multiple crown alpha-helical peptides designed to form artificial ion channels. The approach combines the versatility of solid phase peptide synthesis, the conformational predictability of peptidic molecules, and the solution synthesis of crown ethers with engineerable ion-binding abilities. Several biophysical methods were employed to characterize the activity and the mode of action of these crown peptide nanostructures. The 21 residue peptides bearing six 21-EC-7 turned out to facilitate the translocation of ions in a similar fashion to natural ion channels.
Biron
Eric
E
Département de chimie and Centre de recherche sur la fonction, la structure et l'ingénierie des protéines, Faculté des sciences et de génie, Université Laval, Québec, Canada G1K 7P4.
Otis
François
F
Meillon
Jean-Christophe
JC
Robitaille
Martin
M
Lamothe
Julie
J
Van Hove
Patrick
P
Cormier
Marie-Eve
ME
Voyer
Normand
N
D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D043844:Crown Ethers / Q000138:chemical synthesis* / Q000378:metabolism; D005260:Female; D006801:Humans; D007473:Ion Channels / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D007938:Leukemia / Q000378:metabolism / Q000473:pathology; D008051:Lipid Bilayers / Q000378:metabolism*; D008567:Membranes, Artificial; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000737:chemistry / Q000502:physiology*; D010713:Phosphatidylcholines / Q000378:metabolism; D011487:Protein Conformation; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
D043844:Crown Ethers; D007473:Ion Channels; D008051:Lipid Bilayers; D008567:Membranes, Artificial; D010446:Peptide Fragments; D010713:Phosphatidylcholines