Main alkaloids from the Brazilian dendrobatidae frog Epipedobates flavopictus: pumiliotoxin 251D, histrionicotoxin and decahydroquinolines.
Epipedobates flavopictus, Dendrobatidae, is a small aposematic frog found in Brazilian Cerrado bioma. In the present work, we isolated and characterized chemically the most abundant alkaloids present in the cutaneous extract of E. flavopictus. The specimens were collected in Pirenópolis (Goiás, Brazil), their skins were removed and extracted with methanol, and submitted to purification by HPLC and identification by gas chromatography and mass spectrometry. Pumiliotoxin 251D, histrionicotoxin 285Da and two decahydroquinolines, 219A and 243A, were identified. The pumiliotoxin 251D was tested on isolated frog sciatic nerve and on isolated guinea pig ileum muscle. The pumiliotoxin 251D slightly reduced the action potentials amplitude of frog sciatic nerve. The crude skin extract of E. flavopictus and the pumiliotoxin 251D produced rhythmic contractions and increased the muscular tension on isolated guinea pig ileum.
Mortari
Márcia Renata
MR
Laboratório de Toxinologia, Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade de Brasília, Campus Universitario, Brasília, DF 70910-900, Brazil.
Schwartz
Elisabeth N Ferroni
EN
Schwartz
Carlos Alberto
CA
Pires
Osmindo Rodrigues
OR
Santos
Marcello Moreira
MM
Bloch
Carlos
C
Sebben
Antonio
A
D000470:Alkaloids / Q000737:chemistry* / Q000494:pharmacology; D000664:Amphibian Venoms / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D001001:Anura; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects; D007928:Lethal Dose 50; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D009119:Muscle Contraction / Q000187:drug effects; D012584:Sciatic Nerve / Q000187:drug effects; D012867:Skin / Q000737:chemistry*
D000470:Alkaloids; D000664:Amphibian Venoms