Simulation of (125)I decay in a synthetic oligodeoxynucleotide with normal and distorted geometry and the role of radiation and non-radiation actions.
Within the track structure code PARTRAC, DNA strand break induction by direct and indirect radiation action was calculated for the E. coli catabolite gene activator protein (CAP) DNA complex with (125)I located at the position of the H(5) atom of the cytosine near the center. The shape of the resulting DNA fragment size distributions was found to be in reasonable agreement with corresponding experimental results. However, the calculated yield was considerably lower than the measured one. To study possible reasons for this, recently published experimental data on DNA strand breaks in a 41-mer synthetic oligodeoxynucleotide (oligoDNA) with incorporated (125)I were analyzed aiming at an evaluation of the non-radiation-related component due to the neutralization of the initially highly charged (125m)Te daughter ion. This was done by assuming that the differences between simulated radiation-induced distribution and the measured total fragment size distributions were due to the neutralization process. The neutralization effect defined in this way was found to dominate the strand breakage frequency within a range of 5-7 base pairs around the (125)I decay site on both strands. After implementing this neutralization effect derived from the oligoDNA analysis into the PARTRAC simulation for the CAP-DNA complex, the agreement of the calculated DNA fragment distributions with the corresponding experimental data was considerably improved. The results indicate that DNA conformation may be explored by incorporation of (125)I into the DNA, measurement of fragment size distributions, and comparison with simulation calculation for various hypothetical DNA models.
Li
Wei Bo
WB
Institute of Radiation Protection, GSF-National Research Center for Environment and Health, 85764, Neuherberg, Germany. wli@gsf.de
Friedland
Werner
W
Jacob
Peter
P
Panyutin
Igor G
IG
Paretzke
Herwig G
HG
D003198:Computer Simulation; D002373:Cyclic AMP Receptor Protein / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000528:radiation effects*; D004249:DNA Damage; D053938:DNA Fragmentation; D004583:Electrons; D007457:Iodine Radioisotopes; D008958:Models, Molecular
D002373:Cyclic AMP Receptor Protein; D007457:Iodine Radioisotopes; D004247:DNA