title: Degradation of 2,4,6-tribromophenol by bacterial cells attached to chalk collected from a contaminated aquifer.
abstract: OBJECTIVE
  To investigate the factors governing the adhesion and activity of the 2,4,6-tribromophenol (TBP) degrading bacterium Achromobacter piechaudii TBPZ-N61 on chalk from a contaminated aquifer.
  
  
  RESULTS
  Adhesion kinetics of TBPZ-N61 to grey and white chalk from a polluted fractured chalk aquifer was tested in a batch system. Both grey and white chalk contain ca 80% CaCO3, while grey chalk contains more organic matter (2.4%) than the white chalk (0.3%) and also contains Dolmite and Clinoptilolite. Adhesion of the bacterial cells to the chalk particles (<0.2 mm) occurred rapidly (96% of the cells within 15 min). Langmuir-fitted adhesion isotherms suggest that cells in the stationary phase, which are more hydrophobic, adhere to both grey and white chalk more efficiently than cells in the logarithmic growth phase. Increasing the pH (from 6.7 to 8.1) caused a significant reduction in cell adhesion to the chalk. Activity of attached cells was evaluated in both batch and column experiments. Logarithmic cells adhering to white and grey chalk were more active in TBP degradation than cells in suspension. In column experiments, significant TBP degradation was retained up to 30 days after a single injection of TBPZ cells. Thereafter, activity was fully recovered by amendment of yeast extract. Chalk surfaces that were incubated in situ in contaminated groundwater for 20 days still allowed the adhesion and activity of TBPZ cells.
  
  
  CONCLUSIONS
  Taken together, our results show that bacteria adhere efficiently to specific sites on the chalk surfaces, and that sustained bacterial activity of the attached cells can be achieved by adding a carbon source such as yeast extract which also overcome toxic constituents that may occur in some chalk types.
  
  
  CONCLUSIONS
  Bioremediation of TBP-contaminated chalk aquifers is made possible by the injection of bacterial cultures.
authors.affiliation: Department of Environmental Hydrology and Microbiology, Institute for Water Sciences and Technologies, Ben-Gurion University of the Negev, Sede-Boqer Campus, Medreshet Ben Gurion, Israel. alineji@bgumail.bgu.ac.il
authors.forename: A
authors.initials: A
authors.lastname: Nejidat
authors.forename: I
authors.initials: I
authors.lastname: Saadi
authors.forename: Z
authors.initials: Z
authors.lastname: Ronen
mesh_terms: D042402:Achromobacter / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002119:Calcium Carbonate; D004783:Environmental Microbiology; D019015:Geologic Sediments; D010636:Phenols / Q000378:metabolism*; D000267:Tissue Adhesions; D014877:Water Pollution, Chemical
chemical_list: D010636:Phenols; D002119:Calcium Carbonate; C004554:2,4,6-tribromophenol