Use of Fimers to eliminate polymerase chain reaction and primer-dimer artifacts and to increase yield in BAC-sequencing reactions.
Malykh
Andrei
A
Fidelity Systems Inc., Gaithersburg, MD, USA.
Polushin
Nikolai
N
Slesarev
Alexei
A
Kozyavkin
Sergei
S
D001483:Base Sequence; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D017931:DNA Primers; D019281:Dimerization; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D009841:Oligonucleotides / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods*
D017931:DNA Primers; D009841:Oligonucleotides