Intestinal villus histological alterations in piglets fed dietary charcoal powder including wood vinegar compound liquid.
To investigate the effects of dietary charcoal powder including wood vinegar compound liquid (CWVC, 4 : 1) on intestinal villus histology, piglets were fed 0, 1, 3 and 5% dietary CWVC diets for 30 days. Feed intake and body weight gain were measured during the experimental period. At the end of the experiments, intestinal villus height, epithelial cell area and cell mitosis were examined using light microscopy (LM), and the duodenal villus tip surface was observed using scanning electron microscopy (SEM). Feed efficiency tended to be improved in the CWVC group. The 3% CWVC group showed the highest value, followed by 1% CWVC group of most LM parameters in most intestinal parts, but the 5% CWVC group showed the almost similar value compared with the control. In addition, on the duodenal villus tip surface, the 3% CWVC group showed a clearer cell outline, larger cells and cells protuberated further into the lumen than those of the 1% CWVC group. However, the 5% CWVC group showed faint SEM features than the 1% CWVC group. The present trend of improved feed efficiency after feedings of dietary CWVC demonstrates that the CWVC could be incorporated into piglet diets up to 3% level, and that the CWVC might activate intestinal functions both at villus and cellular levels.
Mekbungwan
A
A
Laboratory of Animal Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa-ken, Japan.
Yamauchi
K
K
Sakaida
T
T
D019342:Acetic Acid / Q000008:administration & dosage*; D000821:Animal Feed; D000818:Animals; D002606:Charcoal / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004386:Duodenum / Q000033:anatomy & histology / Q000187:drug effects / Q000648:ultrastructure*; D004435:Eating / Q000187:drug effects; D007413:Intestinal Mucosa / Q000187:drug effects / Q000648:ultrastructure; D000432:Methanol / Q000008:administration & dosage*; D008855:Microscopy, Electron, Scanning / Q000662:veterinary; D008938:Mitosis; D013552:Swine; D015430:Weight Gain / Q000187:drug effects
D004338:Drug Combinations; D002606:Charcoal; C080339:wood vinegar; D019342:Acetic Acid; D000432:Methanol