title: Mast cells degranulation affects angiogenesis in the rat uterine cervix during pregnancy.
abstract: During pregnancy, it is essential that sufficient nutrients are supplied by the vascular system to support the dramatic modifications of the rat uterine cervix. Angiogenesis refers to the growth of new blood vessels from pre-existing microcirculation and mast cells have been associated with this process. This study examined the modifications of the vascular compartment and the distribution of mast cells on cervical tissue during pregnancy. Using disodium cromoglycate as a mast cell stabilizer, we determined the effects of the mast cell degranulation on cervical angiogenesis. Mast cell distribution and their degranulation status were evaluated by immunohistochemistry. Endothelial cell proliferation was measured by bromodeoxyuridine incorporation. Vascular areas (absolute and relative) and maturation indices were assessed by quantitative immunohistochemistry of von Willebrand factor and alpha-smooth muscle actin respectively. Mast cells were predominantly observed during the first half of pregnancy in the perivascular zones. The values of bromodeoxyuridine incorporation, absolute vascular area and vascular maturation index exhibited a significant increase throughout pregnancy. All animals that received mast cell stabilizer showed more than 40% of non-degranulated mast cells. Treated rats exhibited a decrease in endothelial proliferation and in relative vascular area; in addition, a large proportion of mature blood vessels was observed, suggesting a diminished level of new vessel formation. The effects of the mast cell stabilizer were sustained beyond the end of treatment. This is the first report that brings evidence that mast cell degranulation could be a necessary process to contribute to the normal angiogenesis of the rat cervix during pregnancy. Further investigations are needed to elucidate the possible implications of abnormal vascular development of the uterine cervix on the physiological process of ripening and parturition.
authors.affiliation: Laboratorio de Endocrinología y Tumores Hormonodependientes, School of Biochemistry and Biological Sciences, Universidad Nacional del Litoral, Casilla de Correo 242, Santa Fe, Argentina.
authors.forename: J
authors.initials: J
authors.lastname: Varayoud
authors.forename: J G
authors.initials: JG
authors.lastname: Ramos
authors.forename: V L
authors.initials: VL
authors.lastname: Bosquiazzo
authors.forename: M
authors.initials: M
authors.lastname: Muñoz-de-Toro
authors.forename: E H
authors.initials: EH
authors.lastname: Luque
mesh_terms: D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects; D002455:Cell Division; D002584:Cervix Uteri / Q000098:blood supply* / Q000166:cytology; D004205:Cromolyn Sodium / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology; D005260:Female; D007150:Immunohistochemistry / Q000379:methods; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology*; D018919:Neovascularization, Physiologic; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004205:Cromolyn Sodium