Dynamic expression of bFGF and TGFbeta2 in glomus cell grafts of carotid body in rat model of Parkinson disease.
To investigate the changes in the expression of basic fibroblast growth factor (bFGF) and transforming growth factor beta 2 (TGFbeta2) in glomus cell grafts of carotid body in the rat model of 6-hydroxydopamine-induced Parkinson disease, immunohistochemical staining of bFGF and TGFbeta2 in the sections of striate body was done on the 2nd, 4th and 12th week after transplantation. The results showed that on the 2nd week after transplantation, bFGF and TGFbeta2 were not detectable in the glumous cell grafts. On the 4th week after graft, bFGF and TGFbeta2 immunoreactivity was increased within the grafts and at the graft-host interface but was restricted only to astrocytes. In the striatum surrounding the graft, bFGF was expressed persistently, while TGFbeta2 showed transient expression. It was suggested that the transient expression of TGFbeta2 was likely due more to the trauma imposed by the graft procedure than to an intrinsic. The deficiency in astrocytic bFGF early after graft may be responsible for the poor survival of grafted glomus cells of carotid body.
Cao
Xuebing
X
Department of Neurology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.
Sun
Shenggang
S
Liu
Hongtao
H
Tong
E'tang
E
Xia
Huisheng
H
D000818:Animals; D002344:Carotid Body / Q000166:cytology* / Q000637:transplantation; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis* / Q000235:genetics; D006892:Hydroxydopamines; D010300:Parkinson Disease / Q000209:etiology / Q000378:metabolism* / Q000601:surgery; D051381:Rats; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics; D053781:Transforming Growth Factor beta2; D014184:Transplantation, Homologous
D006892:Hydroxydopamines; D016212:Transforming Growth Factor beta; D053781:Transforming Growth Factor beta2; D016222:Fibroblast Growth Factor 2