Production of hydroxyeicosatetraenoic acids and prostaglandins by a novel rat microglial cell line.
We have established a clonal cell line derived from rat microglia that proliferates in response to macrophage-colony stimulating factor (CSF-1). Like primary neonatal microglia, these cells (named RTMGL1) exhibit a ramified morphology, bind isolectin B4, express CD68 and are weakly positive for CD11b and MHC class II. CSF-1-dependent proliferation requires intact signal transduction through several pathways. RTMGL1 synthesize multiple cyclooxygenase (COX) products including 11- and 15-hydroxyeicosatetraenoic acid (HETE) and express COX-2. RTMGL1 synthesize 5-HETE from arachidonic acid (AA) likely via a 5-lipoxygenase (LO). Thus, RTMGL1 have morphological and histological characteristics of primary microglia and metabolize AA via both COX and LO pathways.
Rademacher
David J
DJ
Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA.
Kearn
Christopher S
CS
Carrier
Erica J
EJ
Patel
Sachin
S
Delgado
Maria A
MA
Barkmeier
Andrew
A
Klick
David E
DE
Breese
Nicole M
NM
Pfister
Sandra L
SL
Nithipatikom
Kasem
K
Campbell
William B
WB
Hillard
Cecilia J
CJ
D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D037102:Lectins / Q000378:metabolism; D016173:Macrophage Colony-Stimulating Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology; D013058:Mass Spectrometry / Q000379:methods; D017628:Microglia / Q000378:metabolism*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011453:Prostaglandins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013936:Thymidine / Q000493:pharmacokinetics; D013997:Time Factors; D014316:Tritium / Q000493:pharmacokinetics; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D004791:Enzyme Inhibitors; D005904:Glial Fibrillary Acidic Protein; D006893:Hydroxyeicosatetraenoic Acids; D037102:Lectins; D011453:Prostaglandins; D014409:Tumor Necrosis Factor-alpha; D014316:Tritium; D016173:Macrophage Colony-Stimulating Factor; D011451:Prostaglandin-Endoperoxide Synthases; D013936:Thymidine