title: Anti-venom potential of butanolic extract of Eclipta prostrata against Malayan pit viper venom.
abstract: The butanolic and purified butanolic extracts (PBEs) of Eclipta prostrata were evaluated for their anti-venom potential. Inhibition of lethal, hemorrhagic, proteolytic, and phospholipase A2 activities of Calloselasma rhodostoma (Malayan pit viper (MPV)) venom by these extracts were determined. Demethylwedelolactone was identified as their major constituent. The butanolic extract, at 2.5 mg per mouse, was able to completely neutralize the lethal activity of 2LD50 of MPV venom, but increasing the dose diminished the effect. The PBE, at 1.5-4.5 mg per mouse, was able to neutralize the lethality of the venom at around 50-58%. Both extracts partially inhibited the hemorrhagic activity but displayed very low anti-phospholipase A2 activity and did not inhibit proteolytic activity of MPV venom.
authors.affiliation: Faculty of Pharmacy, Mahidol University, Bangkok, Thailand. pypph@hotmail.com
authors.forename: Pimolpan
authors.initials: P
authors.lastname: Pithayanukul
authors.forename: Sasitorn
authors.initials: S
authors.lastname: Laovachirasuwan
authors.forename: Rapepol
authors.initials: R
authors.lastname: Bavovada
authors.forename: Narumol
authors.initials: N
authors.lastname: Pakmanee
authors.forename: Rutt
authors.initials: R
authors.lastname: Suttisri
mesh_terms: D000818:Animals; D000997:Antivenins / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D000440:Butanols / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D003435:Crotalid Venoms / Q000037:antagonists & inhibitors* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D036601:Eclipta; D005260:Female; D006470:Hemorrhage / Q000139:chemically induced / Q000188:drug therapy; D008297:Male; D051379:Mice; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D017819:Viperidae
chemical_list: D000997:Antivenins; D000440:Butanols; D003435:Crotalid Venoms; D010936:Plant Extracts