title: LPS-induced microvascular leukocytosis can be assessed by blue-field entoptic phenomenon.
abstract: Administration of low doses of Escherichia coli endotoxin [a lipopolysaccharide (LPS)] to humans enables the study of inflammatory mechanisms. The purpose of the present study was to investigate whether the blue-field entoptic technique may be used to quantify the increase in circulating leukocytes in the ocular microvasculature after LPS infusion. In addition, combined laser Doppler velocimetry and retinal vessel size measurement were used to study red blood cell movement. Twelve healthy male volunteers received 20 IU/kg iv LPS as a bolus infusion. Outcome parameters were measured at baseline and 4 h after LPS administration. In the first protocol (n = 6 subjects), ocular hemodynamic effects were assessed with the blue-field entoptic technique, the retinal vessel analyzer, and laser Doppler velocimetry. In the second protocol (n = 6 subjects), white blood cell (WBC) counts from peripheral blood samples and blue-field entoptic technique measurements were performed. LPS caused peripheral blood leukocytosis and increased WBC density in ocular microvessels (by 49%; P = 0.036) but did not change WBC velocity. In addition, retinal venous diameter was increased (by 9%; P = 0.008), but red blood cell velocity remained unchanged. The LPS-induced changes in retinal WBC density and leukocyte counts were significantly correlated (r = 0.87). The present study indicates that the blue-field entoptic technique can be used to assess microvascular leukocyte recruitment in vivo. In addition, our data indicate retinal venous dilation in response to endotoxin.
authors.affiliation: Department of Clinical Pharmacology, University of Vienna, Vienna, Austria.
authors.forename: Julia
authors.initials: J
authors.lastname: Kolodjaschna
authors.forename: Fatmire
authors.initials: F
authors.lastname: Berisha
authors.forename: Solveig
authors.initials: S
authors.lastname: Lung
authors.forename: Georg
authors.initials: G
authors.lastname: Schaller
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Polska
authors.forename: Bernd
authors.initials: B
authors.lastname: Jilma
authors.forename: Michael
authors.initials: M
authors.lastname: Wolzt
authors.forename: Leopold
authors.initials: L
authors.lastname: Schmetterer
mesh_terms: D000328:Adult; D001783:Blood Flow Velocity / Q000187:drug effects; D002465:Cell Movement; D004912:Erythrocytes / Q000187:drug effects; D005123:Eye / Q000098:blood supply*; D006801:Humans; D017078:Laser-Doppler Flowmetry; D007958:Leukocyte Count; D007964:Leukocytosis / Q000097:blood / Q000139:chemically induced* / Q000473:pathology / Q000503:physiopathology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D012171:Retinal Vessels / Q000473:pathology / Q000503:physiopathology; D014664:Vasodilation
chemical_list: D008070:Lipopolysaccharides