Spontaneous apoptosis in the intra-ductal component's stroma of breast invasive carcinoma.
Spontaneous apoptosis by in situ detection of DNA fragmentation (DNAf) was investigated in breast invasive ductal carcinoma (IDC) frozen samples removed from 61 untreated patients. The incidence of DNAf was low in carcinoma cells and was mainly detected in the stroma. In the stroma at a distance from carcinoma cells, DNAf was inversely related to estradiol plasma level variations (p=0.01), indicating that it probably remained under physiological hormonal regulation. In the stroma adjacent to carcinoma cells, DNAf was correlated to tumor progression parameters such as the presence of a comedo intra ductal carcinoma (DCIS) component (p=0.001) and axillary lymph node metastasis (p=0.002), suggesting that this stromal compartment more probably represented a tumoral component closely associated to epithelial tumor cells. Therefore, the detection of DNAf in the adjacent stroma of breast carcinoma could help to predict progression in non invasive tumors and also in invasive tumors in those patients without lymph node invasion.
Rouleau-Dubois
Caroline
C
Laboratoire d'Anatomie-Pathologique, Hôpital Lapeyronie, 371 avenue du Doyen Gaston Giraud, 34295 Montpellier, Cedex 1, France. c-dubois@chu-montpellier.fr
Cuny
Marguerite
M
Ferrieres
Gaelle
G
Simony-Lafontaine
Joelle
J
Rouanet
Philippe
P
Grenier
Jean
J
Jeanteur
Philippe
P
Baldet
Pierre
P
Escot
Chantal
C
D000328:Adult; D001943:Breast Neoplasms / Q000473:pathology*; D044584:Carcinoma, Ductal / Q000473:pathology*; D053938:DNA Fragmentation / Q000502:physiology*; D005260:Female; D006801:Humans; D017154:Stromal Cells / Q000473:pathology