title: Ternatin, a flavonoid, prevents cyclophosphamide and ifosfamide-induced hemorrhagic cystitis in rats.
abstract: To compare the classical uroprotective efficacy of mesna (2-mercaptoethanesulfonic acid) with ternatin (flavonoid isolated from Egletes viscosa Less.) in cyclophosphamide (CYP) and ifosfamide (IFS) induced hemorrhagic cystitis (HC). Male Wistar rats received an intraperitoneal injection of saline, CYP or IFS and were treated with saline or mesna, 5 min before, 4 and 8 h after CYP or IFS administration. In other animals, 1, 2 or 3 doses of mesna were replaced with ternatin or 3 doses of mesna were replaced with dimethylsulphoxide (DMSO), ternatin diluent. In an additional group, the last 2 doses of mesna were replaced with saline. HC was evaluated 24 h after CYP or IFS administration. CYP or IFS treatment induced marked changes in macroscopic and microscopic evaluation and in bladder wet weight (BWW), and these alterations were significantly inhibited by treatment with 3 doses of mesna, as well as by the replacement of 1 or 2 doses of mesna with ternatin. The replacement of 2 doses of mesna with saline or all doses of mesna with ternatin or DMSO did not prevent HC. In conclusion, the replacement of 1 or 2 doses of mesna with ternatin efficiently blocked CYP- or IFS-induced HC, however mesna is necessary for initial uroprotection.
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Brazil.
authors.forename: Milena Morais
authors.initials: MM
authors.lastname: Vieira
authors.forename: Francisco Yuri B
authors.initials: FY
authors.lastname: Macêdo
authors.forename: José Nelson Belarmino
authors.initials: JN
authors.lastname: Filho
authors.forename: Ana Carolina L V
authors.initials: AC
authors.lastname: Costa
authors.forename: Adriano N
authors.initials: AN
authors.lastname: Cunha
authors.forename: Edilberto R
authors.initials: ER
authors.lastname: Silveira
authors.forename: Gerly Anne C
authors.initials: GA
authors.lastname: Brito
authors.forename: Ronaldo A
authors.initials: RA
authors.lastname: Ribeiro
mesh_terms: D000818:Animals; D019659:Asteraceae; D003520:Cyclophosphamide; D003556:Cystitis / Q000139:chemically induced / Q000517:prevention & control*; D005419:Flavonoids / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D035264:Flowers; D006470:Hemorrhage / Q000139:chemically induced / Q000517:prevention & control; D007069:Ifosfamide; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D020011:Protective Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D001743:Urinary Bladder / Q000187:drug effects / Q000473:pathology
chemical_list: D005419:Flavonoids; D010936:Plant Extracts; D020011:Protective Agents; C051080:ternatin (flavonoid); D003520:Cyclophosphamide; D007069:Ifosfamide