[Comparative study on antibacterial effects of huangqin-tang and its metabolites produced by intestinal flora].
OBJECTIVE
To compare the antibacterial effects of Huangqin-Tang with its metabolites produced by intestinal flora.


METHODS
The antibacterial tests in vitro and in vivo were performed by agar dilution method and lethal protection of animal respectively.


RESULTS
Huangqin-Tang and its metabolites had antibacterial action on bacteria in vitro, however the antibacterial activity of metabolites of Huangqin-Tang on Salmomella, Dysentery bacillus and Proteus in vitro was stronger than Huangqin-Tang. The metabolites of Huangqin-Tang had protective effect on the animals infected by Staphylococcus aureus and Escherichia coli respectively from death, but Huangqin-Tang had no lethal protection action.


CONCLUSIONS
The antibacterial effects of metabolites of Huangqin-Tang in vitro and in vivo are stronger than that of Huangqin-Tang, which shows that intestinal flora play a very important role in antibacterial effects of Huangqin-Tang.
Yan
Mei-zhen
MZ
Institute of Chinese Material Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Zuo
Feng
F
Song
Hong-yue
HY
Ye
Wen-hua
WH
Zhou
Zhong-ming
ZM
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D004927:Escherichia coli Infections / Q000188:drug therapy; D005243:Feces / Q000382:microbiology; D007422:Intestines / Q000382:microbiology*; D008297:Male; D051379:Mice; D008517:Phytotherapy; D010946:Plants, Medicinal / Q000737:chemistry; D011511:Proteus / Q000187:drug effects; D012475:Salmonella / Q000187:drug effects*; D012762:Shigella dysenteriae / Q000187:drug effects; D013203:Staphylococcal Infections / Q000188:drug therapy*
D000900:Anti-Bacterial Agents; D004365:Drugs, Chinese Herbal; C439205:Huangqin-Tang