title: Immunohistochemical localization of phospholipase D2 in embryonic rat brain.
abstract: The present study has characterized the cellular and temporal localization of the phospholipase D2 (PLD2) protein in the embryonic rat brain, using immunohistochemistry. PLD2 immunoreactivity was first observed in the choroid plexus and in the most ventricular zone of the lateral and third ventricles at embryonic day 15 (E15), followed by gradual restriction to the limited zone of ventricles at E20. In addition, PLD2 expression was high in the developing cerebral cortex and hippocampus. In the cortex, PLD2 expression was observed in the marginal zone from the earliest stage (E15) and then declined and had completely disappeared by E20. Double-labelling studies demonstrated co-expression of the anti-class III beta-tubulin antibody in most of the PLD2 immunoreactive cells. Therefore, our findings suggest that PLD2 may be involved in early developmental processes of some neuronal progenitors.
authors.affiliation: Department of Anatomy, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Socho-gu, 137-701 Seoul, South Korea.
authors.forename: Jeong-Sun
authors.initials: JS
authors.lastname: Choi
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Park
authors.forename: Young Chan
authors.initials: YC
authors.lastname: Jo
authors.forename: Myung-Hoon
authors.initials: MH
authors.lastname: Chun
authors.forename: Jin-Woong
authors.initials: JW
authors.lastname: Chung
authors.forename: Joon Mo
authors.initials: JM
authors.lastname: Kim
authors.forename: Do Sik
authors.initials: do S
authors.lastname: Min
authors.forename: Mun-Yong
authors.initials: MY
authors.lastname: Lee
mesh_terms: D000818:Animals; D001921:Brain / Q000196:embryology* / Q000201:enzymology*; D002465:Cell Movement / Q000502:physiology; D005260:Female; D010739:Phospholipase D / Q000032:analysis* / Q000096:biosynthesis*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C105606:phospholipase D2; D010739:Phospholipase D