title: Performance characteristics of updated INNO-LiPA assays for molecular typing of human leukocyte antigen A (HLA-A), HLA-B, and HLA-DQB1 alleles.
abstract: We carried out a multicenter performance evaluation of three new DNA-based human leukocyte antigen (HLA) typing assays: INNO-LiPA HLA-A Update, INNO-LiPA HLA-B Update, and INNO-LiPA HLA-DQB1 Update. After optimization, the accuracy rates were all 100%, and the final observed resolutions were 99.4, 92.4, and 85.6%, respectively. These rapid and easy-to-perform assays yielded results fully concordant with other DNA-based tissue typing tests.
authors.affiliation: Innogenetics N.V., B-9052 Ghent, Belgium. Karen_De_Vreese@innogenetics.be
authors.forename: Karen
authors.initials: K
authors.lastname: De Vreese
authors.forename: Rachel
authors.initials: R
authors.lastname: Barylski
authors.forename: Fiona
authors.initials: F
authors.lastname: Pughe
authors.forename: Miriam
authors.initials: M
authors.lastname: Bläser
authors.forename: Colin
authors.initials: C
authors.lastname: Evans
authors.forename: John
authors.initials: J
authors.lastname: Norton
authors.forename: Gilbert
authors.initials: G
authors.lastname: Semana
authors.forename: Richard
authors.initials: R
authors.lastname: Holman
authors.forename: Pascale
authors.initials: P
authors.lastname: Loiseau
authors.forename: Dominique
authors.initials: D
authors.lastname: Masson
authors.forename: Mieke
authors.initials: M
authors.lastname: Gielis
authors.forename: Annelies
authors.initials: A
authors.lastname: De Brauwer
authors.forename: Ilse
authors.initials: I
authors.lastname: De Canck
authors.forename: Gonda
authors.initials: G
authors.lastname: Verpooten
authors.forename: Frank
authors.initials: F
authors.lastname: Hulstaert
mesh_terms: D005787:Gene Frequency; D015234:HLA-A Antigens / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans
chemical_list: D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen