Preparation and usage of a new solid phase micro-extraction membrane.
Liquid-liquid or solid phase extraction methods are widely used for isolating analytes from urine, blood and other samples. But the preparation procedures of the samples are laborious, intensive, and costly. In addition, the organic solvents used are toxic to both the human body and the environment. An accurate, simple and rapid method for analysis of some compounds is required for forensic, judicial, and clinical purposes. Solid phase micro-extraction membrane (SPMEM) is a new, simple and solventless preparation technique. It integrates sampling, extraction and concentration into a single step and has the advantages of both the solid phase micro-extraction (SPME) and membrane separation. In this paper, a new kind of membrane used for the solid phase micro-extraction was prepared with amide compounds. The extraction conditions such as adsorption time, desorption solvents, methods and time are studied and optimized. The dichlorvos in the blood, morphine and phenobarbital in the urine were perfectly separated by using this solid phase micro-extraction membrane, and were tested by gas chromatograph-mass spectrometer (GC-MS). All the data were acquired in scan mode except that of morphine which was obtained in a selected ion monitoring (SIM) mode. Ions used for identification were those with m/z 57, 115, 162, 215, 285.
Yang
Ruiqin
R
Department of Forensic Science, Chinese People's Public Security University, Beijing 100038, China. rrqyang66@yahoo.com.cn
Xie
Wenlin
W
D000327:Adsorption; D000577:Amides / Q000737:chemistry*; D004006:Dichlorvos / Q000097:blood; D005554:Forensic Medicine; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006993:Hypnotics and Sedatives / Q000652:urine; D007306:Insecticides / Q000097:blood; D008567:Membranes, Artificial; D008832:Microchemistry / Q000379:methods*; D009020:Morphine / Q000652:urine; D009294:Narcotics / Q000652:urine; D010634:Phenobarbital / Q000652:urine
D000577:Amides; D006993:Hypnotics and Sedatives; D007306:Insecticides; D008567:Membranes, Artificial; D009294:Narcotics; D009020:Morphine; D004006:Dichlorvos; D010634:Phenobarbital