The N-terminal domain of the replication initiator protein RepE is a dimerization domain forming a stable dimer.
The initiator protein RepE of the mini-F plasmid in Escherichia coli plays an essential role in DNA replication, which is regulated by the molecular chaperone-dependent oligomeric state (monomer or dimer). Crosslinking, ultracentrifugation, and gel filtration analyses showed that the solely expressed N-terminal domain (residues 1-144 or 1-152) exists in the dimeric state as in the wild-type RepE protein. This result indicates that the N-terminal domain functions as a dimerization domain of RepE and might be important for the interaction with the molecular chaperones. The N-terminal domain dimer has been crystallized in order to obtain structural insight into the regulation of the monomer/dimer conversion of RepE.
Nakamura
Akira
A
Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
Komori
Hirofumi
H
Kobayashi
Gengo
G
Kita
Akiko
A
Wada
Chieko
C
Miki
Kunio
K
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D002850:Chromatography, Gel; D003432:Cross-Linking Reagents / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray; D004265:DNA Helicases / Q000737:chemistry* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics; D019281:Dimerization; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins; D008958:Models, Molecular; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012097:Repressor Proteins / Q000737:chemistry* / Q000235:genetics; D015534:Trans-Activators / Q000737:chemistry* / Q000235:genetics; D014461:Ultracentrifugation / Q000379:methods
D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; C074164:RepE protein, E coli; D012097:Repressor Proteins; D015534:Trans-Activators; C045264:replication initiator protein; D004265:DNA Helicases