A patch-clamp investigation of membrane currents in a novel mammalian retinal ganglion cell line.
We characterised membrane currents in undifferentiated RGC-5 cells, a cell line used in in vitro models of apoptosis and glaucoma. The cells were inexcitable, with no voltage-dependent Na(+) currents or action potentials. Some novel currents were observed including basal Cl(-) currents, inwardly rectifiying K(+) currents and Gd(3+) insensitive stretch-activated currents. Our results highlight the differences between the electrophysiological properties of undifferentiated RGC-5 cells and retinal ganglion cells.
Moorhouse
Andrew J
AJ
School of Medical Sciences, The University of New South Wales, Sydney, 2052, Australia.
Li
Shaojuan
S
Vickery
Richard M
RM
Hill
Mark A
MA
Morley
John W
JW
D000818:Animals; D002460:Cell Line; D007473:Ion Channels / Q000502:physiology; D008564:Membrane Potentials / Q000502:physiology; D018408:Patch-Clamp Techniques / Q000379:methods; D012165:Retinal Ganglion Cells / Q000166:cytology* / Q000502:physiology*
D007473:Ion Channels