Ontogenic and adult whole body distribution of aminopeptidase N in rat investigated by in vitro autoradiography.
Aminopeptidase N (APN), which is widely distributed in mammalian tissues, is able to cleave numerous regulatory peptides. The selective inhibitor of APN, [(125)I] RB129, has been used to study the distribution of this exopeptidase during rat prenatal development and adult life by in vitro whole-body autoradiography. In the central nervous system, APN shows a weak labeling compared to the major part of the non-nervous tissues in the embryo and in the adult. APN is progressively expressed in kidney, intestine, heart, lung, sensory organs, eye, and thymus. In organs such as the liver, the cartilages and the bones, altered levels of APN expression are observed during the development, or in the embryo compared to the adult, suggesting a role of APN during the liver haematopoiesis and bone growth. At this time, all the physiological functions of APN are still incompletely known, however its developmental pattern of expression strongly suggests a function of modulation of this enzyme during the development, next in physiological and/or pathological situations in adult. In this way, APN could represent a new therapeutic target in pathological processes, such as tumoral proliferation and/or angiogenesis associated with cancer development, where an increase in the level of this enzyme has been observed.
Jardinaud
Fanny
F
Département de Pharmacochimie Moléculaire & Structurale, Inserm U266, CNRS FRE 2463, UFR des Sciences Pharmaceutiques & Biologiques, 4, avenue de l'Observatoire, 75270 Paris cedex, France.
Banisadr
Ghasal
G
Noble
Florence
F
Mélik-Parsadaniantz
Stéphane
S
Chen
Huixiong
H
Dugave
Christophe
C
Laplace
Hervé
H
Rostène
William
W
Fournié-Zaluski
Marie-Claude
MC
Roques
Bernard P
BP
Popovici
Théodora
T
D000367:Age Factors; D000818:Animals; D001345:Autoradiography / Q000379:methods; D018826:CD13 Antigens / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000378:metabolism*; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005260:Female; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes / Q000737:chemistry; D008297:Male; D007470:Monoiodotyrosine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D011480:Protease Inhibitors / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
C404566:2(S)-benzyl-3-hydroxy(1'(R)-aminoethyl)phosphinylpropanoyl-L-3-iodotyrosine; D007457:Iodine Radioisotopes; D011480:Protease Inhibitors; D018826:CD13 Antigens; D007470:Monoiodotyrosine