Effect of Acanthopanax senticosus on lipoprotein lipase in 3T3-L1 adipocytes.
The effect of Acanthopanax senticosus (AS) leaves on lipoprotein lipase (LPL) was investigated in 3T3-L1 adipocytes. A water extract of AS leaves increased the LPL activity in culture medium of adipocytes in a dose- and time-dependent manner. The AS extract contained heparin-like LPL releasing components, however, the increase of medium LPL activity was continued up until 12 h, in contrast to the rapid decline after heparin treatment. The increase of LPL mRNA was also observed after AS extract treatment, suggesting that LPL induction occurs at the transcriptional level. The AS extract could partially reverse the LPL suppression by tumour necrosis factor-alpha in 3T3-L1 adipocytes. These results of an AS extract-induced increase of LPL activity in vitro suggest the possible action of AS as a facilitator of plasma triglyceride clearance.
Yang
Jeong-Yeh
JY
Department of Biochemistry, Chonbuk National University Medical School, Jeonju, Korea.
Lee
Kang-Seop
KS
Kim
Myung-Kon
MK
Moon
Seong-Keun
SK
Kang
Mi-Kyung
MK
Park
Byung-Hyun
BH
Kim
Jong-Suk
JS
Park
Jin-Woo
JW
D016475:3T3 Cells / Q000187:drug effects; D017667:Adipocytes / Q000187:drug effects; D000818:Animals; D017931:DNA Primers; D028001:Eleutherococcus; D015228:Hypertriglyceridemia / Q000517:prevention & control; D000960:Hypolipidemic Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D008071:Lipoprotein Lipase / Q000187:drug effects*; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves; D012333:RNA, Messenger / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D000960:Hypolipidemic Agents; D010936:Plant Extracts; D012333:RNA, Messenger; D008071:Lipoprotein Lipase