Recombinant avian adenovirus CELO expressing the human interleukin-2: characterization in vitro, in ovo and in vivo.
In our study, a recombinant adenovirus based on the avian adenovirus CELO genome, has been constructed that contains the human interleukin-2 gene. We have shown the production of biologically active recombinant interleukin-2 in vitro (LMH and 293 cells) and in ovo (chicken embryos) infected with recombinant virus CELO-IL2. An increase in the median survival time of C57BL/6 mice carrying B16 melanoma tumors has been demonstrated after multiple intra-tumors injections of the recombinant adenovirus CELO-IL2.
Cherenova
L V
LV
Gamaleya Research Institute of Epidemiology and Microbiology, Gamaleya Street 18, 123098 Moscow, Russia. cherenova@aport2000.ru
Logunov
D Y
DY
Shashkova
E V
EV
Shmarov
M M
MM
Verkhovskaya
L V
LV
Neugodova
G L
GL
Kazansky
D B
DB
Doronin
K K
KK
Naroditsky
B S
BS
D000818:Animals; D002642:Chick Embryo / Q000821:virology; D003001:Cloning, Molecular / Q000379:methods; D005260:Female; D025681:Fowl adenovirus A / Q000235:genetics*; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics* / Q000627:therapeutic use; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010957:Plasmids; D016019:Survival Analysis
D007376:Interleukin-2