Hypoxic stress stably alters apoptotic parameters on U937 cells.
Tumor promonocytic U937 cells cultured under a low O(2)/high CO(2) atmosphere display altered characteristics after restoration of normal atmosphere: increased resistance to apoptosis induced by different treatments; apoptotic morphology; lack of glutathione (GSH) extrusion in apoptosis; lack of protection by antioxidants; and lack of Ca(2+) mobilization with thapsigargin. These alterations were stably maintained for many months of culture in normal conditions, originating the stable U937-HX variant. Since the hypoxic treatment did not produce a great selective pressure, the alterations are conceivably the result of stable adaptative response.
De Nicola
M
M
Dipartimento di Biologia, Università di Roma Tor Vergata, via della Ricerca Scientifica, 00133 Roma, Italy. milden78@libero.it
Liuzzi
F
F
Cerella
C
C
D'Alessio
M
M
Bergamaschi
A
A
Magrini
A
A
Ghibelli
L
L
D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D020013:Calcium Signaling / Q000502:physiology; D015687:Cell Hypoxia / Q000502:physiology*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D011691:Puromycin / Q000494:pharmacology*; D020298:U937 Cells
D000975:Antioxidants; D011691:Puromycin; D005978:Glutathione