title: Quantitative age-related changes in dorsal lateral geniculate nucleus relay neurons of the rat.
abstract: An ultrastructural and quantitative study of the age-related changes occurring in the relay neurons of the dorsal lateral geniculate nucleus (dLGN) was carried out using male Wistar rats aged 3, 18, 24, and 28 months. Morphometric techniques were used to obtain data regarding cellular activity including soma, nuclear, and nucleolar size. Volume fractions for rough endoplasmic reticulum (RER), mitochondria, and lipofuscin, as well as numbers and sizes of mitochondria and dense bodies (DB) was also calculated. Among the few alterations found in the perikaryon, we can highlight the redistribution and fragmentation of RER and an increase and progressive aggregation of lipofuscin. Quantitative data show a significant decrease in the volume of the soma (-42.77%) and the nucleus (-33.66%), and in the volume fraction of the RER (-18.81%) and mitochondria (-10.16%). A significant increase in lipofuscin (+213.29%), and variations in size and number of mitochondria and dense bodies were also found. Some histophysiological considerations about the findings are discussed. The findings lead to the conclusion that a relative degree of morphological stability is exhibited by relay neurons, although the quantitative data show evident intracellular changes, especially from 24 to 28 months. These changes suggest that accompanying physiological alterations may occur, with putative effects on visual function during ageing.
authors.affiliation: Department of Histology and Pathology, Faculty of Medicine, University of Málaga, Campus de Teatinos, 29071 Málaga, Spain. lvidal@uma.es
authors.forename: Lourdes
authors.initials: L
authors.lastname: Vidal
authors.forename: Concepción
authors.initials: C
authors.lastname: Ruíz
authors.forename: Alicia
authors.initials: A
authors.lastname: Villena
authors.forename: Florentina
authors.initials: F
authors.lastname: Díaz
authors.forename: Ignacio
authors.initials: I
authors.lastname: Pérez de Vargas
mesh_terms: D000344:Afferent Pathways / Q000648:ultrastructure; D000375:Aging; D000818:Animals; D005829:Geniculate Bodies / Q000473:pathology* / Q000648:ultrastructure; D002479:Inclusion Bodies / Q000378:metabolism / Q000473:pathology; D008062:Lipofuscin / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D009474:Neurons / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar
chemical_list: D008062:Lipofuscin