title: Diagnosis by polymerase chain reaction of ocular posttransplant lymphoproliferative disorder after pediatric renal transplantation.
abstract: OBJECTIVE
  To report diagnosis by polymerase chain reaction of intraocular posttransplant lymphoproliferative disorder in a pediatric renal transplant patient.
  
  
  METHODS
  Observational case report.
  
  
  METHODS
  Retrospective review.
  
  
  RESULTS
  An 11-year-old girl developed infectious mononucleosis 15 months after renal transplantation for focal segmental sclerosis. Papillitis and hypopyon uveitis developed 8 months later, followed by iris nodules. Diagnosis of intraocular posttransplant lymphoproliferative disorder was made by polymerase chain reaction of aqueous humor for Epstein-Barr virus and confirmed by histopathology of an iris biopsy specimen. Infiltrating iris lymphocytes in the biopsy specimen were positive for Epstein-Barr DNA. Polymerase chain reaction also revealed gene rearrangement of the variable region of the heavy immunoglobulin chain, consistent with a monoclonal B-lymphocyte population. Iris nodules resolved with reduction in immunosuppressive medication.
  
  
  CONCLUSIONS
  Polymerase chain reaction for Epstein-Barr virus may be helpful in diagnosis of intraocular posttransplant lymphoproliferative disorder.
authors.affiliation: Department of Ophthalmology, University of Miami School of Medicine, Bascom Palmer Eye Institute, Miami, Florida 33136, USA.
authors.forename: Luma
authors.initials: L
authors.lastname: Al-Attar
authors.forename: Audina
authors.initials: A
authors.lastname: Berrocal
authors.forename: Roberto
authors.initials: R
authors.lastname: Warman
authors.forename: Sander
authors.initials: S
authors.lastname: Dubovy
authors.forename: Ana
authors.initials: A
authors.lastname: Paredes
authors.forename: Chi-Chao
authors.initials: CC
authors.lastname: Chan
authors.forename: Janet
authors.initials: J
authors.lastname: Davis
mesh_terms: D001082:Aqueous Humor / Q000821:virology; D002648:Child; D004279:DNA, Viral / Q000032:analysis; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis / Q000821:virology*; D015828:Eye Infections, Viral / Q000175:diagnosis / Q000821:virology*; D005260:Female; D015321:Gene Rearrangement; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007499:Iris Diseases / Q000175:diagnosis / Q000821:virology; D016030:Kidney Transplantation; D008232:Lymphoproliferative Disorders / Q000175:diagnosis / Q000821:virology*; D016133:Polymerase Chain Reaction; D011183:Postoperative Complications; D012189:Retrospective Studies
chemical_list: D004279:DNA, Viral; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region