The effect of extracellular substance components on the proliferation and expression of hormones in cultured cells of medullary thyroid carcinomas.
The effects were examined of selected extracellular medium (ECM) components on the proliferation of medullary thyroid carcinoma cells and on the production of calcitonin and CGRP. Human TT cells and rat rMTC cells were cultured for 24, 48 and 72 hours on glass coated with type I collagen, fibronectin or poly-D-lysine. More pronounced proliferation was demonstrated by TT cells grown on poly-Dlysine or collagen in comparison with the control and less pronounced proliferation was typical of cells grown on fibronectin. On the other hand, rMTC cells were more markedly manifest on any ECM substrates than that on glass. Alterations in the proliferation were paralleled by changes in the expression of CT and CGRP.
Zabel
Maciej
M
Department of Histology and Embryology, University School of Medicine, Wrocław, Poland. mazab@hist.am.wroc.pl
Gebarowska
Elzbieta
E
Surdyk-Zasada
Joanna
J
Lesisz
Izabela
I
Kasprzak
Aldona
A
Seidel
Jolanta
J
D000818:Animals; D002116:Calcitonin / Q000378:metabolism*; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D018276:Carcinoma, Medullary / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D024042:Collagen Type I / Q000494:pharmacology; D016326:Extracellular Matrix Proteins / Q000494:pharmacology*; D005353:Fibronectins / Q000494:pharmacology; D006801:Humans; D007150:Immunohistochemistry; D039001:Integrin alpha Chains / Q000378:metabolism; D011107:Polylysine / Q000494:pharmacology; D051381:Rats; D013964:Thyroid Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
D024042:Collagen Type I; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D039001:Integrin alpha Chains; D011107:Polylysine; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide