title: Reduced gingival fluid flow: a peripheral marker of the pharmacological effect of roquinimex.
abstract: OBJECTIVE
  Roquinimex is a drug with effects on inflammation and tumors. The pharmacological effect is not fully understood, and the molecular mechanism most characterized in vitro is an increase of plasminogen activator inhibitor type 2 (PAI-2) in human peripheral blood monocytes. The aims were to investigate peripheral pharmacological effects of roquinimex on peripheral blood monocytes and dog gingival fluid (GCF).
  
  
  METHODS
  Six dogs were used in a cross-over study. The amount of GCF was determined with a Periotron. The PAI-2 concentration in GCF was determined with ELISA. Monocytes were isolated from peripheral blood.
  
  
  RESULTS
  Dogs treated with the drug had significantly lower GCF flow values and the PAI-2 concentration in GCF was higher, but no effect was seen on peripheral monocytes.
  
  
  CONCLUSIONS
  Roquinimex treatment led to a consistently decreased flow rate of GCF and a higher local concentration of PAI-2 in GCF.
authors.affiliation: School of Dentistry, Odontological Faculty, Malmö University College, S-205 06 Malmö, Sweden.
authors.forename: Pia
authors.initials: P
authors.lastname: Lindberg
authors.forename: Anita
authors.initials: A
authors.lastname: Billström
authors.forename: Bertil
authors.initials: B
authors.lastname: Kinnby