title: Phylogenetic analysis of the genera Streptomyces and Kitasatospora based on partial RNA polymerase beta-subunit gene (rpoB) sequences.
abstract: The RNA polymerase beta-subunit genes (rpoB) of 67 Streptomyces strains, representing 57 species, five Kitasatospora strains and Micromonospora echinospora KCTC 9549 were partially sequenced using a pair of rpoB PCR primers. Among the streptomycetes, 99.7-100 % similarity within the same species and 90.2-99.3 % similarity at the interspecific level were observed by analysis of the determined rpoB sequences. The topology of the phylogenetic tree based on rpoB sequences was similar to that of 16S rDNA. The five Kitasatospora strains formed a stable monophyletic clade and a sister group to the clade comprising all Streptomyces species. Although there were several discrepancies in the details, considerable agreement was found between the results of rpoB analysis and those of numerical phenetic classification. This study demonstrates that analysis of rpoB can be used as an alternative genetic method in parallel to conventional taxonomic methods, including numerical phenetic and 16S rDNA analyses, for the phylogenetic analyses of the genera Streptomyces and Kitasatospora.
authors.affiliation: Department of Microbiology, College of Medicine, Seoul National University, Seoul 110-799, Korea.
authors.forename: Bum-Joon
authors.initials: BJ
authors.lastname: Kim
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Yusung, Taejon 305-600, Korea.
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Kim
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul 151-742, Korea.
authors.forename: Jongsik
authors.initials: J
authors.lastname: Chun
authors.affiliation: Department of Food Science and Engineering, Cheju National University, Jeju 690-756, Korea.
authors.forename: Young-Hwan
authors.initials: YH
authors.lastname: Koh
authors.affiliation: Department of Microbiology, College of Medicine, Konkuk University, Chungju 380-230, Korea.
authors.forename: Sueng-Hyun
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Biochemistry, College of Medicine, Cheju National University, Jeju 690-756, Korea.
authors.forename: Jin-Won
authors.initials: JW
authors.lastname: Hyun
authors.affiliation: Department of Microbiology, College of Medicine, Seoul National University, Seoul 110-799, Korea.
authors.forename: Chang-Yong
authors.initials: CY
authors.lastname: Cha
authors.affiliation: Department of Microbiology, College of Medicine, Seoul National University, Seoul 110-799, Korea.
authors.forename: Yoon-Hoh
authors.initials: YH
authors.lastname: Kook
mesh_terms: D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013302:Streptomyces / Q000145:classification* / Q000235:genetics; D013306:Streptomycetaceae / Q000145:classification* / Q000235:genetics
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit