[Laboratory study of the yi-fu-ning soft gelatin capsules in treating climacteric syndrome].
OBJECTIVE
To study the therapeutic mechanism of YFN on climacteric syndrome by investigating the effect of Yi-Fu-Ning soft gelatin capsules (YFN), which can nourish the kidney and activate blood, on serum sex hormone levels and hypothalamic monoamine transmitters contents in ovariectomized rats.


METHODS
Fifty female mature Sprague-Dawley rats were randomly divided into 5 groups:sham operation;ovariectomy(OVX);OVX with diethylstilbestrol tablets(DT);OVX with YFN(high dose and low dose). After drugs had been given for 4 weeks, hypothalamic monoamine transmitters (NE, DA, 5-HT, 5-HIAA) contents were detected by fluorospectrophotometric method and serum sex hormone by radioimmunoassay. Adrenal gland index, uterus index and thymus index were also determined.


RESULTS
YFN could obviously improve serum E2 and increase adrenal gland and uterus index in OVX rats (P < 0.01 or P < 0.05). It could reduce hypothalamic 5-HT and 5-HIAA, which were increased in OVX rats(P < 0.01); It could also increase reduced NE and DA in OVX rats and reduce the ratio of 5-HT to NE and 5-HT to DA(P < 0.01).


CONCLUSIONS
YFN can regulate procreate endocrine in various ways and ameliorate the function of hypothalamic monoamine neurotransmitter in OVX rats. So it can stabilize inner condition of body and relieve symptoms of climacteric syndrome.
Xiao
Wei
W
Department of Chinese Traditional Medicine, First Military Medicine University, Guangzhou 510515, Guangdong, China.
Deng
Hong-zhu
HZ
Ma
Yun
Y
Chen
Yu-yao
YY
D000818:Animals; D002214:Capsules; D002979:Climacteric / Q000378:metabolism; D030024:Curcuma / Q000737:chemistry; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D012739:Gonadal Steroid Hormones / Q000097:blood; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D010052:Ovariectomy; D010057:Oviducts / Q000737:chemistry; D011897:Random Allocation; D011898:Ranidae; D051381:Rats; D017207:Rats, Sprague-Dawley
D002214:Capsules; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D012739:Gonadal Steroid Hormones; D008419:Materia Medica