Apoptotic mitochondrial dysfunction induced by benzo(a)pyrene in liver epithelial cells: role of p53 and pHi changes.
How pH(i) changes, more specifically alkalinization, affect the apoptotic cascade has yet to be determined. The aim of the present work was to test the involvement of mitochondria in the apoptotic cascade triggered by benzo(a)pyrene [B(a)P] and to determine the role of pH(i) changes and p53 relative to mitochondria. Our results indicate that B(a)P-induced apoptosis might rely upon a p53-dependent and a pH-sensitive mitochondrial dysfunction.
Huc
Laurence
L
INSERM U456, Faculté de Pharmacie, Université Rennes I, 2 avenue Prof. Léon Bernard, 35043 Rennes cedex, France.
Gilot
David
D
Gardyn
Claire
C
Rissel
Mary
M
Dimanche-Boitrel
Marie-Thérèse
MT
Guillouzo
André
A
Fardel
Olivier
O
Lagadic-Gossmann
Dominique
D
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D001564:Benzo(a)pyrene / Q000633:toxicity*; D052160:Benzothiazoles; D001865:Bongkrekic Acid / Q000494:pharmacology; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D006146:Guanidines / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure*; D051381:Rats; D013450:Sulfones / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D014050:Toluene / Q000031:analogs & derivatives* / Q000494:pharmacology; D016159:Tumor Suppressor Protein p53 / Q000187:drug effects
D052160:Benzothiazoles; D006146:Guanidines; D013450:Sulfones; D013844:Thiazoles; D016159:Tumor Suppressor Protein p53; D001865:Bongkrekic Acid; D001564:Benzo(a)pyrene; D014050:Toluene; C093373:cariporide; C121565:pifithrin