title: Pulp revascularization of replanted immature dog teeth after treatment with minocycline and doxycycline assessed by laser Doppler flowmetry, radiography, and histology.
abstract: This study investigated the effect of topical antibiotic treatment on pulp revascularization in replanted teeth. Thirty-four immature teeth were selected from three young dogs. Baseline radiographs and laser Doppler flowmetry (LDF) readings were obtained. Specimens were randomly divided into four groups: Thirty-eight teeth were extracted, kept dry for 5 min, and either (Group 1) covered with minocycline mixture (G1, n = 11), (Group 2) soaked in doxycycline (G2, n = 11), or (Group 3) soaked in saline (G3-negative control, n = 6), and replanted. Teeth in Group 4 were not extracted (positive control, n = 6). Postoperative radiographs and LDF readings were obtained for 2 months after replantation. After sacrifice, the jaws were collected and processed for light microscopy. Pre- and postreplantation LDF readings and radiographs, and histologic findings were analyzed to assess revascularization. Pulp revascularization occurred in 91% (G1), 73% (G2), and 33% (G3) of the specimens. In conclusion, minocycline facilitates pulp revascularization in replanted immature teeth after replantation.
authors.affiliation: Department of Endodontics, School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7450, USA. Alessandra.Ritter@dentistry.unc.edu
authors.forename: Alessandra Luisa de Souza
authors.initials: AL
authors.lastname: Ritter
authors.forename: André Vicente
authors.initials: AV
authors.lastname: Ritter
authors.forename: Valerie
authors.initials: V
authors.lastname: Murrah
authors.forename: Asgeir
authors.initials: A
authors.lastname: Sigurdsson
authors.forename: Martin
authors.initials: M
authors.lastname: Trope
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage; D003782:Dental Pulp / Q000098:blood supply* / Q000502:physiology; D004285:Dogs; D004318:Doxycycline / Q000008:administration & dosage; D017078:Laser-Doppler Flowmetry; D008911:Minocycline / Q000008:administration & dosage; D018919:Neovascularization, Physiologic; D011859:Radiography; D012038:Regeneration; D012388:Root Canal Irrigants / Q000008:administration & dosage; D014084:Tooth Avulsion / Q000601:surgery*; D014090:Tooth Replantation; D014092:Tooth Root / Q000000981:diagnostic imaging / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D000891:Anti-Infective Agents, Local; D012388:Root Canal Irrigants; D008911:Minocycline; D004318:Doxycycline