Mechanistic insights into the kinetics of HIV-1 nucleocapsid protein-facilitated tRNA annealing to the primer binding site.
HIV-1 reverse transcriptase uses human tRNA(Lys,3) as a primer to initiate reverse transcription. Prior to initiation, the 3' 18 nucleotides of this tRNA are annealed to a complementary sequence on the RNA genome known as the primer binding site (PBS). Here, we show that the HIV-1 nucleocapsid protein (NC) enhances this annealing by approximately five orders of magnitude in vitro, decreasing the transition state enthalpy from approximately 20 kcal mol(-1) for the uncatalyzed reaction to 13 kcal mol(-1) for the NC-catalyzed process. Moreover, the annealing follows second-order kinetics, consistent with the nucleation of the intermolecular duplex being the rate-limiting step. This nucleation is preceded by melting of a small duplex region within the original structure, and is followed by much faster zipping of the rest of the 18 base-pair (bp) duplex. A tRNA mutational analysis shows that destabilization of the tRNA acceptor stem has only a minor effect on the annealing rate. In contrast, addition of bases to the 5' end of tRNA that are complementary to its single-stranded 3' end interferes with duplex nucleation and therefore has a much larger effect on the net reaction rate. Assuming that the apparent transition free energy of the annealing reaction, Delta G(++) is a sum of the melting (Delta G(m)) and nucleation (Delta G(nuc)) free energies, we show that NC affects both Delta G(m) and Delta G(nuc). We estimate that ten to 100-fold of the overall rate enhancement is due to NC-induced destabilization of a 4 bp helix in the PBS, while the additional factor of 10(3)-10(4) rate enhancement is a result of NC-facilitated duplex nucleation. The apparently similar effectiveness of wild-type and SSHS NC, a mutant that lacks the zinc finger structures, in facilitating the tRNA annealing reaction is most likely the result of the mutual cancellation of two factors: SSHS NC is less effective than wild-type NC as a duplex destabilizer, but more effective as a duplex nucleating agent.
Hargittai
Michele R S
MR
Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA.
Gorelick
Robert J
RJ
Rouzina
Ioulia
I
Musier-Forsyth
Karin
K
D001665:Binding Sites; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers / Q000378:metabolism*; D015497:HIV-1 / Q000235:genetics*; D007700:Kinetics; D009154:Mutation; D012343:RNA, Transfer / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D016335:Zinc Fingers / Q000235:genetics / Q000502:physiology
D036022:Capsid Proteins; D017931:DNA Primers; D012343:RNA, Transfer