Purification, crystallization and preliminary X-ray diffraction analysis of human oncoprotein SET/TAF-1beta.
The human oncoprotein SET/TAF-1beta has been crystallized by the sitting-drop vapour-diffusion method using ammonium sulfate as a precipitant. The crystal belongs to space group C2, with unit-cell parameters a = 119.6, b = 62.8, c = 61.0 A, beta = 89.7 degrees, and contains two molecules in the asymmetric unit. A complete data set was collected to 2.8 A resolution using synchrotron radiation.
Muto
Shinsuke
S
Horikoshi Gene Selector Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Corporation (JST), 5-9-6 Tokodai, Tsukuba, Ibaraki 300-2635, Japan.
Senda
Miki
M
Adachi
Naruhiko
N
Suzuki
Toru
T
Nagai
Ryozo
R
Senda
Toshiya
T
Horikoshi
Masami
M
D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry*; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins; D056488:Histone Chaperones; D006801:Humans; D014157:Transcription Factors / Q000737:chemistry*
D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D056488:Histone Chaperones; C513473:SET protein, human; D014157:Transcription Factors