title: Poly(L-lysine)-modified silica nanoparticles for the delivery of antisense oligonucleotides.
abstract: Silica nanoparticles were prepared in a microemulsion system, using polyoxyethylene nonylphenyl ether/cyclohexane/ammonium hydroxide. The surface charge of the particle was modified with PLL [poly(L-lysine)]. PAGE demonstrated the ability of PMS-NP (PLL-modified silica nanoparticles) to bind and protect antisense ODNs (oligonucleotides). The intracellular localization of FITC-labelled ODN was investigated by fluorescence microscopy. The results demonstrated that ODN could be delivered to cytoplasm. Flow-cytometry analysis showed a 20-fold enhancement of ODN delivered by PMS-NP compared with free ODN for a serum-free medium. Blocking efficacy of c- myc antisense ODN, delivered by PMS-NP, was examined in HNE1 and HeLa cell lines. Significant down-regulation of c- myc mRNA levels was observed in both the cell lines. However, the cellular uptake efficiency and antisense effects on target gene decreased in the presence of serum-containing medium. The analysis of the filtration assay showed that PMS-NP interacted with serum proteins. These results indicated that PMS-NP was a suitable delivery vector for antisense ODN, although its delivery efficiency decreased in the presence of a serum-containing medium.
authors.affiliation: Cancer Research Institute, XiangYa School of Medicine, Central South University, ChangSha, HuNan 410078, People's Republic of China.
authors.forename: Shi-Guo
authors.initials: SG
authors.lastname: Zhu
authors.forename: Juan-Juan
authors.initials: JJ
authors.lastname: Xiang
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Li
authors.forename: Shou-Rong
authors.initials: SR
authors.lastname: Shen
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Lu
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.forename: Wei
authors.initials: W
authors.lastname: Xiong
authors.forename: Bi-Cheng
authors.initials: BC
authors.lastname: Zhang
authors.forename: Xin-Min
authors.initials: XM
authors.lastname: Nie
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
authors.forename: Ke
authors.initials: K
authors.lastname: Tang
authors.forename: Gui-Yuan
authors.initials: GY
authors.lastname: Li
mesh_terms: D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D018014:Gene Transfer Techniques; D006367:HeLa Cells; D006801:Humans; D008422:Materials Testing; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D020320:Oligoribonucleotides, Antisense / Q000008:administration & dosage* / Q000737:chemistry / Q000235:genetics / Q000493:pharmacokinetics; D010316:Particle Size; D011107:Polylysine / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D014018:Tissue Distribution; D014162:Transfection / Q000379:methods*
chemical_list: D020099:Coated Materials, Biocompatible; D004337:Drug Carriers; D020320:Oligoribonucleotides, Antisense; D011107:Polylysine; D012822:Silicon Dioxide