[Crystalline nickel sulfide-induced genomic instability in transformed human broncho-epithelial cells].
OBJECTIVE
To detect the genomic instability in the 16 human broncho-epithelial (16HBE) cells induced by crystalline nickel sulfide so as to provide the scientific basis for further study of nickel-induced cancer molecular mechanism.


METHODS
To analyse the genomic instability in transformed 16HBE cells induced by crystalline nickel sulfide by random amplified polymorphic DNA (RAPD).


RESULTS
All the 7 random primers selected could amplify 1 - 6 clear PCR bands. There were no significant differences between transformed 16HBE cells and negative control cells in the 4th, 5th, and 7th primers, but in the rest 4 primers there were significant differences, with special PCR bands for the same primer, indicating that genomic instability in transformed 16 HBE cells was induced by crystalline nickel sulfide.


CONCLUSIONS
Crystalline nickel sulfide could induce genomic instability in 16HBE cells.
Chen
Chuan-de
CD
Center for Disease Control and Prevention of Luohu District, Shenzhen 518020, China.
Wu
Zhong-liang
ZL
Chen
Jia-kun
JK
Ji
Wei-dong
WD
D002461:Cell Line, Transformed; D003460:Crystallization; D004247:DNA / Q000187:drug effects / Q000235:genetics; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D042822:Genomic Instability / Q000187:drug effects*; D006801:Humans; D009532:Nickel / Q000633:toxicity*; D019105:Random Amplified Polymorphic DNA Technique
D009532:Nickel; D004247:DNA; C017558:nickel sulfide