Mitochondrial membrane potential after low-power laser irradiation.
We used the lipophilic cationic fluorescent dye 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimidazol-carbocyanine iodide (JC-1) to determine mitochondrial membrane potential (mdeltapsi) in Hep-2 cells after irradiation with low-power laser (lambda=635 nm). Through this methodology it was possible to analyze the variation on mitochondrial number and mdeltapsi, in cells irradiated for 100, 150 and 200 s with energy density of 100 mJ/cm(2). Our results show that JC-1 dye allows the identification of populations with different mitochondria morphology as well as the functionality of this organelle in the cells incubated for 1, 6 and 24 h, after irradiation with low-power laser.
Bortoletto
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Laboratório de Biologia Celular e Tecidual, Instituto de Pesquisa e Desenvolvimento, Universidade do Vale do Paraíba, Av. Shishima Hifumi 2911, 12244-000, São José dos Campos, S P, Brazil.
Silva
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NS
Zângaro
R A
RA
Pacheco
M T T
MT
Da Matta
R A
RA
Pacheco-Soares
C
C
D001562:Benzimidazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D006801:Humans; D028022:Low-Level Light Therapy / Q000379:methods*; D008564:Membrane Potentials; D008566:Membranes / Q000502:physiology; D008928:Mitochondria / Q000502:physiology*
D001562:Benzimidazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; C068624:5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine