Peptide-protein microarrays for the simultaneous detection of pathogen infections.
We describe novel peptide-protein microarrays, which were fabricated using semicarbazide glass slides that permitted the immobilization of glyoxylyl peptides by site-specific ligation and the immobilization of proteins by physisorption. The arrays permitted the simultaneous serodetection of antibodies directed against hepatitis C virus (HCV core p21 15-45 peptide, NS4 1925-1947 peptide, core, NS3, NS4, and mixture of core, NS3, NS4, and NS5 antigens), hepatitis B virus (HBc, HBe, and HBs), human immunodeficiency virus (Gp41 and Gp120 for HIV-I and Gp36 for HIV-II), Epstein-Barr virus (VCAp18 153-176 peptide), and syphilis (rTpN47 and rTpN17) antigens using an immunofluorescence assay. Peptide-protein microarrays displayed high signal-to-noise ratios, sensitivities, and specificities for the detection of antibodies as revealed by the analysis of a collection of human sera referenced against these five pathogens.
Duburcq
Xavier
X
UMR CNRS 8527, Biological Institute of Lille, 1 Rue du Pr Calmette, 59021 Lille, France.
Olivier
Christophe
C
Malingue
Frédéric
F
Desmet
Rémi
R
Bouzidi
Ahmed
A
Zhou
Fenhling
F
Auriault
Claude
C
Gras-Masse
Hélène
H
Melnyk
Oleg
O
D003141:Communicable Diseases / Q000175:diagnosis / Q000382:microbiology* / Q000821:virology*; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis / Q000378:metabolism / Q000821:virology; D006505:Hepatitis / Q000175:diagnosis / Q000378:metabolism / Q000821:virology; D006518:Hepatitis Viruses / Q000235:genetics / Q000302:isolation & purification; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D010455:Peptides / Q000737:chemistry / Q000235:genetics; D040081:Protein Array Analysis / Q000379:methods*; D012698:Serologic Tests / Q000379:methods; D014210:Treponema pallidum / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification
D010455:Peptides