Effect of Kuibitang on lipopolysaccharide-induced cytokine production in peripheral blood mononuclear cells of chronic fatigue syndrome patients.
Kuibitang (KBT) is clinically used to treat patients suffering from chronic fatigue syndrome (CFS) in South Korea. However, its effect has not been investigated experimentally. Recent reports have shown that CFS patients display an altered cytokine production. We examined the effect of KBT on lipopolysaccharide (LPS)-induced various cytokines production in peripheral blood mononuclear cells (PBMC) of CFS patients and healthy controls. KBT (1 mg/ml) significantly inhibited LPS-induced tumor necrosis factor-alpha, interleukin-10, and transforming growth factor-beta1 production in PBMC of CFS patients. However, LPS-induced interferon-gamma production was significantly increased by KBT (0.01 mg/ml). These results provide evidence of a novel activity of the KBT that regulate cytokines production related with CFS.
Shin
Hye-Young
HY
Department of Pharmacology, College of Oriental Medicine, Kyung Hee University, Seoul, South Korea.
An
Nyeon-Hyoung
NH
Cha
Yun-Jin
YJ
Shin
Eun-Ju
EJ
Shin
Tae-Yong
TY
Baek
Seung-Hwa
SH
Kim
Cheorl-Ho
CH
Lyu
Yeung-Su
YS
Lee
Eon-Jeong
EJ
Kim
Hyung-Min
HM
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000328:Adult; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D016207:Cytokines / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D004306:Dose-Response Relationship, Immunologic; D015673:Fatigue Syndrome, Chronic / Q000188:drug therapy / Q000378:metabolism*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008516:Medicine, Chinese Traditional; D020835:Medicine, Kampo; D008875:Middle Aged; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D018709:Statistics, Nonparametric
D000276:Adjuvants, Immunologic; D016207:Cytokines; D008070:Lipopolysaccharides; D010936:Plant Extracts