Use of whole blood dried on filter paper for detection and genotyping of measles virus.
PCR, and two different ELISAs were used to detect measles virus on blood dried on filter paper samples from Equatorial Guinea. Sensitivity was 40% by PCR, 57.1% by indirect ELISA and 86.7% by micro chain capture ELISA. Genotype B3 was found in two positive samples by PCR.
Mosquera
María del Mar
Mdel M
Centro Nacional de Microbiología, Instituto de Salud Carlos III, Carretera de Majadahonda-Pozuelo s/n, 28220 Majadahonda, Madrid, Spain. mmosquera@isciii.es
Echevarría
Juan E
JE
Puente
Sabino
S
Lahulla
Félix
F
de Ory
Fernando
F
D001800:Blood Specimen Collection / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005838:Genotype; D006801:Humans; D007223:Infant; D008459:Measles virus / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity