title: Melanocins A, B and C, new melanin synthesis inhibitors produced by Eupenicillium shearii. I. Taxonomy, fermentation, isolation and biological properties.
abstract: New melanin synthesis inhibitors, melanocins A, B and C, were isolated from the fermentation broth and mycelium extract of Eupenicillium shearii F80695. Melanocin A, an isocyanide compound, inhibited mushroom tyrosinase and melanin biosynthesis of B16 melanoma cells with IC50 value of 9.0 nM and MIC value of 0.9 microM, respectively. Melanocin A also inhibited growth of Streptomyces bikiniensis. While, the structurally very related but non-isocyanide compounds melanocins B and C did not show inhibitory activity in these assays. Melanocins A, B and C showed potent antioxidant activity with scavenging activity of DPPH radical and superoxide anion radical.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Yuseong, Daejeon 305-600, Korea.
authors.forename: Jong-Pyung
authors.initials: JP
authors.lastname: Kim
authors.forename: Byoung-Kwon
authors.initials: BK
authors.lastname: Kim
authors.forename: Bong-Sik
authors.initials: BS
authors.lastname: Yun
authors.forename: In-Ja
authors.initials: IJ
authors.lastname: Ryoo
authors.forename: Choong Hwan
authors.initials: CH
authors.lastname: Lee
authors.forename: In-Kyoung
authors.initials: IK
authors.lastname: Lee
authors.forename: Won-Gon
authors.initials: WG
authors.lastname: Kim
authors.forename: Sangku
authors.initials: S
authors.lastname: Lee
authors.forename: Yu-Ryang
authors.initials: YR
authors.lastname: Pyun
authors.forename: Ick-Dong
authors.initials: ID
authors.lastname: Yoo
mesh_terms: D000363:Agaricales / Q000201:enzymology; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology; D001203:Ascomycota / Q000145:classification / Q000378:metabolism*; D002074:Butanones / Q000302:isolation & purification / Q000494:pharmacology; D003486:Cyanides / Q000302:isolation & purification / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D005559:Formamides / Q000302:isolation & purification / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D008543:Melanins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors; D013302:Streptomyces / Q000187:drug effects / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D002074:Butanones; D003486:Cyanides; D004791:Enzyme Inhibitors; D005559:Formamides; D016166:Free Radical Scavengers; D008543:Melanins; C484096:melanocin A; C484095:melanocin B; C484097:melanocin C; D014442:Monophenol Monooxygenase