title: Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus.
abstract: A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection.
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Shannon L
authors.initials: SL
authors.lastname: Emery
authors.forename: Dean D
authors.initials: DD
authors.lastname: Erdman
authors.forename: Michael D
authors.initials: MD
authors.lastname: Bowen
authors.forename: Bruce R
authors.initials: BR
authors.lastname: Newton
authors.forename: Jonas M
authors.initials: JM
authors.lastname: Winchell
authors.forename: Richard F
authors.initials: RF
authors.lastname: Meyer
authors.forename: Suxiang
authors.initials: S
authors.lastname: Tong
authors.forename: Byron T
authors.initials: BT
authors.lastname: Cook
authors.forename: Brian P
authors.initials: BP
authors.lastname: Holloway
authors.forename: Karen A
authors.initials: KA
authors.lastname: McCaustland
authors.forename: Paul A
authors.initials: PA
authors.lastname: Rota
authors.forename: Bettina
authors.initials: B
authors.lastname: Bankamp
authors.forename: Luis E
authors.initials: LE
authors.lastname: Lowe
authors.forename: Tom G
authors.initials: TG
authors.lastname: Ksiazek
authors.forename: William J
authors.initials: WJ
authors.lastname: Bellini
authors.forename: Larry J
authors.initials: LJ
authors.lastname: Anderson
mesh_terms: D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D006801:Humans; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000706:statistics & numerical data; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D045169:Severe Acute Respiratory Syndrome / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology; D014709:Vero Cells
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D012367:RNA, Viral