Rhodococcus gordoniae sp. nov., an actinomycete isolated from clinical material and phenol-contaminated soil.
The taxonomic relationships of two actinomycetes provisionally assigned to the genus Rhodococcus were determined using a polyphasic taxonomic approach. The generic assignment was confirmed by 16S rRNA gene similarity data, as the organisms, strains MTCC 1534 and W 4937(T), were shown to belong to the Rhodococcus rhodochrous subclade. These organisms had phenotypic properties typical of rhodococci; they were aerobic, Gram-positive, weakly acid-fast actinomycetes that showed an elementary branching-rod-coccus growth cycle and contained meso-diaminopimelic acid, arabinose and galactose in whole-organism hydrolysates, N-glycolated muramic acid residues, dehydrogenated menaquinones with eight isoprene units as the predominant isoprenologue and mycolic acids that co-migrated with those extracted from the type strain of R. rhodochrous. The strains had identical phenotypic profiles and belong to the same genomic species, albeit one distinguished from Rhodococcus pyridinivorans, with which they formed a distinct phyletic line. They were also distinguished from representatives of all of the species classified in the R. rhodochrous 16S rRNA gene tree using a set of phenotypic features. The genotypic and phenotypic data show that the strains merit recognition as a novel species of Rhodococcus. The name proposed is Rhodococcus gordoniae sp. nov., with the type strain W 4937(T) (=DSM 44689(T)=NCTC 13296(T)).
Jones
Amanda L
AL
Department of Microbiology, Freeman Hospital, Newcastle upon Tyne NE7 7DN, UK.|School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
Brown
June M
JM
Meningitis and Special Pathogens Branch, Division of Bacterial and Mycotic Diseases Branch, Division of AIDS, STD and TB Laboratory, National Centre for Infectious Diseases, Atlanta, GA 30333, USA.
Mishra
Vachaspati
V
Molecular and Cellular Biochemistry, The Ohio State University, 485 Hamilton Hall, 1645 Neil Avenue, Columbus, OH 43210, USA.
Perry
John D
JD
Department of Microbiology, Freeman Hospital, Newcastle upon Tyne NE7 7DN, UK.
Steigerwalt
Arnold G
AG
Meningitis and Special Pathogens Branch, Division of Bacterial and Mycotic Diseases Branch, Division of AIDS, STD and TB Laboratory, National Centre for Infectious Diseases, Atlanta, GA 30333, USA.
Goodfellow
Michael
M
School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D006801:Humans; D010636:Phenols; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012988:Soil Microbiology; D012989:Soil Pollutants; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010636:Phenols; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants