title: A new role for the mitotic RAD21/SCC1 cohesin in meiotic chromosome cohesion and segregation in the mouse.
abstract: The evolutionarily conserved cohesin complex is required for the establishment and maintenance of sister chromatid cohesion, in turn essential for proper chromosome segregation. RAD21/SCC1 is a regulatory subunit of the mitotic cohesin complex, as it links together all other subunits of the complex. The destruction of RAD21/SCC1 along chromosomal arms and later at centromeres results in the dissociation of the cohesin complex, facilitating chromosome segregation. Here, we report for the first time that mammalian RAD21/SCC1 associates with the axial/lateral elements of the synaptonemal complex along chromosome arms and on centromeres of mouse spermatocytes. Importantly, RAD21/SCC1 is lost from chromosome arms in late prophase I but persists on centromeres. The loss of centromeric RAD21/SCC1 coincides with the separation of sister chromatids at anaphase II. These findings support a role for mammalian RAD21/SCC1 in maintaining sister chromatid cohesion in meiosis.
authors.affiliation: Divisions of Research and Radiation Oncology, Peter MacCallum Cancer Centre, East Melbourne, Victoria 3002, Australia.
authors.forename: Huiling
authors.initials: H
authors.lastname: Xu
authors.forename: Matthew
authors.initials: M
authors.lastname: Beasley
authors.forename: Sandra
authors.initials: S
authors.lastname: Verschoor
authors.forename: Amy
authors.initials: A
authors.lastname: Inselman
authors.forename: Mary Ann
authors.initials: MA
authors.lastname: Handel
authors.forename: Michael J
authors.initials: MJ
authors.lastname: McKay
mesh_terms: D000705:Anaphase / Q000502:physiology; D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002503:Centromere / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone; D020090:Chromosome Segregation / Q000502:physiology*; D002875:Chromosomes / Q000378:metabolism*; D004268:DNA-Binding Proteins; D008297:Male; D008540:Meiosis / Q000502:physiology; D051379:Mice; D008938:Mitosis / Q000502:physiology*; D009687:Nuclear Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D011418:Prophase / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins; D013737:Testis / Q000166:cytology / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; C490529:MCD1 protein, S cerevisiae; D009687:Nuclear Proteins; D010750:Phosphoproteins; C465862:Rad21 protein, mouse; D029701:Saccharomyces cerevisiae Proteins