title: Role of protein-protein interactions during herpes simplex virus type 1 recombination-dependent replication.
abstract: Recombination-dependent replication is an integral part of the process by which double-strand DNA breaks are repaired to maintain genome integrity. It also serves as a means to replicate genomic termini. We reported previously on the reconstitution of a recombination-dependent replication system using purified herpes simplex virus type 1 proteins (Nimonkar A. V., and Boehmer, P. E. (2003) Proc. Natl. Acad. Sci. U. S. A. 100, 10201-10206). In this system, homologous pairing by the viral single-strand DNA-binding protein (ICP8) is coupled to DNA synthesis by the viral DNA polymerase and helicase-primase in the presence of a DNA-relaxing enzyme. Here we show that DNA synthesis in this system is dependent on the viral polymerase processivity factor (UL42). Moreover, although DNA synthesis is strictly dependent on topoisomerase I, it is only stimulated by the viral helicase in a manner that requires the helicase-loading protein (UL8). Furthermore, we have examined the dependence of DNA synthesis in the viral system on species-specific protein-protein interactions. Optimal DNA synthesis was observed with the herpes simplex virus type 1 replication proteins, ICP8, DNA polymerase (UL30/UL42), and helicase-primase (UL5/UL52/UL8). Interestingly, substitution of each component with functional homologues from other systems for the most part did not drastically impede DNA synthesis. In contrast, recombination-dependent replication promoted by the bacteriophage T7 replisome was disrupted by substitution with the replication proteins from herpes simplex virus type 1. These results show that although DNA synthesis performed by the T7 replisome is dependent on cognate protein-protein interactions, such interactions are less important in the herpes simplex virus replisome.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, Florida 33101-6129, USA.
authors.forename: Amitabh V
authors.initials: AV
authors.lastname: Nimonkar
authors.forename: Paul E
authors.initials: PE
authors.lastname: Boehmer
mesh_terms: D017123:Bacteriophage T7 / Q000378:metabolism; D004247:DNA / Q000737:chemistry; D004265:DNA Helicases / Q000737:chemistry / Q000502:physiology; D019915:DNA Primase; D004268:DNA-Binding Proteins; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005090:Exodeoxyribonucleases / Q000378:metabolism; D018259:Herpesvirus 1, Human / Q000201:enzymology / Q000235:genetics*; D009841:Oligonucleotides / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011995:Recombination, Genetic; D013997:Time Factors; D014764:Viral Proteins / Q000378:metabolism / Q000502:physiology; D014779:Virus Replication
chemical_list: D004268:DNA-Binding Proteins; C045462:ICP8 protein, Simplexvirus; D009841:Oligonucleotides; D014764:Viral Proteins; D004247:DNA; D019915:DNA Primase; C034970:bacteriophage T7 induced DNA polymerase; D004259:DNA-Directed DNA Polymerase; D005090:Exodeoxyribonucleases; C062021:helicase-primase, Human herpesvirus 1; C043417:DNA polymerase, Simplexvirus; D004265:DNA Helicases