A study on a chitosan-gelatin-hyaluronic acid scaffold as artificial skin in vitro and its tissue engineering applications.
Chitosan-gelatin-hyaluronic acid scaffolds for tissue regeneration were fabricated by freezing and lyophilizing methods. The scaffolds showed a higher water uptake and retention abilities than chitosan-gelatin scaffolds did. Fibroblasts cultured in chitosan-gelatin-hyaluronic acid scaffolds grew and proliferated well, and they exhibited a strong viability. Keratinocytes were co-cultured with fibroblasts in chitosan-gelatin-hyaluronic acid scaffolds to construct an artificial bilayer skin in vitro. The artificial skin obtained was flexible and had good mechanical properties. The data from this study suggested that chitosan-gelatin-hyaluronic acid scaffolds are suitable for preparing a bilayer skin substitute.
Liu
Haifeng
H
Research Institute of Polymer Materials, Tianjin University, Tianjin 300072, Peoples Republic of China.
Mao
Jinshu
J
Yao
Kangde
K
Yang
Guanghui
G
Cui
Lei
L
Cao
Yilin
Y
D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry; D002448:Cell Adhesion; D002452:Cell Count; D002455:Cell Division; D002478:Cells, Cultured; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry*; D048271:Chitosan; D018920:Coculture Techniques; D003094:Collagen / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D005612:Freeze Drying; D005615:Freezing; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000166:cytology / Q000648:ultrastructure; D008297:Male; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D017768:Skin, Artificial; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D014867:Water / Q000737:chemistry; D017155:Wettability
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D014867:Water; D002686:Chitin; D005780:Gelatin; D006820:Hyaluronic Acid; D003094:Collagen; D048271:Chitosan