[Co-expression of two spliceosomes of cyclin-dependent kinase-2 in SH-SY5Y cells].
OBJECTIVE
To investigate the expression of cyclin-dependent kinase-2 (CDK-2) gene in SH-SY5Y cells.


METHODS
The expression of CDK-2 gene was examined with reverse transcriptional (RT)-PCR, and the PCR products underwent electrophoresis on non-denaturing poly-acrylamide gel (PAG) followed by silver staining. The separated and purified DNAs were ligated into pMD18-T vector, and the positive clones identified by sequence analysis.


RESULTS
Two DNA bands were displayed on PAG, and the one with smaller molecular weight was less intensively stained. The sequences of the two clones indicated that both were products of CDK-2 gene.


CONCLUSIONS
Two kinds of CDK-2 gene products are co-expressed in the SH-SY5Y cells, one of which lacks the fifth exon and is expressed at a low level.
Yue
Feng
F
Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China. yue-feng@fimmu.com
Ma
Wen-li
WL
Zhang
Bao
B
Song
Yan-bin
YB
Zheng
Wen-ling
WL
D042846:CDC2-CDC28 Kinases / Q000235:genetics*; D045744:Cell Line, Tumor; D051357:Cyclin-Dependent Kinase 2; D005434:Flow Cytometry; D006801:Humans; D009447:Neuroblastoma / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D017381:Spliceosomes
D042846:CDC2-CDC28 Kinases; C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2