title: Viral DNA polymerase scanning and the gymnastics of Sendai virus RNA synthesis.
abstract: mRNA synthesis from nonsegmented negative-strand RNA virus (NNV) genomes is unique in tht the genome RNA is embedded in an N protein assembly (the nucleocapsid) and the viral RNA polymerase does not dissociate from the template after release of each mRNA, but rather scans the genome RNA for the next gene-start site. A revised model for NNV RNA synthesis is presented, in which RNA polymerase scanning plays a prominent role. Polymerase scanning of the template is known to occur as the viral transcriptase negotiates gene junctions without falling off the template.
authors.affiliation: Department of Genetics and Microbiology, University of Geneva School of Medicine, Switzerland. Daniel.Kolakofsky@medecine.unige.ch
authors.forename: Daniel
authors.initials: D
authors.lastname: Kolakofsky
authors.forename: Philippe
authors.initials: P
authors.lastname: Le Mercier
authors.forename: Frédéric
authors.initials: F
authors.lastname: Iseni
authors.forename: Dominique
authors.initials: D
authors.lastname: Garcin
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000378:metabolism*; D019590:Nucleocapsid Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis*; D029082:Sendai virus / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics
chemical_list: D019590:Nucleocapsid Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases