Processing of photoaptamer microarrays.
Photoaptamers are single-stranded nucleic acids selected for their high affinity to specific proteins of interest. Photoaptamer microarrays capture and quantify proteins from complex samples using a unique protocol that leverages both high-affinity capture with covalent retention of analytes. The initial capture of proteins from solution is similar to the well-known antibody capture, but the "secondary binding event" affected by photoaptamers is a covalent crosslink between the photoaptamer capture agent and the protein analyte. The nature of this specific covalent reaction allows a unique microarray processing that is described in detail in this chapter.
Petach
Helen
H
SomaLogic Inc, Boulder, CO, USA.
Ostroff
Rachel
R
Greef
Chad
C
Husar
Gregory M
GM
D003432:Cross-Linking Reagents / Q000378:metabolism; D043169:Endostatins / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D008027:Light; D009696:Nucleic Acids / Q000378:metabolism*; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011485:Protein Binding
D003432:Cross-Linking Reagents; D043169:Endostatins; D005456:Fluorescent Dyes; D009696:Nucleic Acids