title: Senescence-associated decline of lymphocyte migration in gut-associated lymphoid tissues of rat small intestine.
abstract: Scenescence-induced changes in gut-associated lymphoid tissues may contribute largely to the impaired immune responses during aging. Age-related changes in lymphocyte recirculations were investigated in Peyer's patches of rat small intestine. Cell dynamics of labeled T lymphocytes were observed under an intravital fluorescence microscope and compared between young and aged rats. Lymphocyte transport through intestinal lymph was decreased in aged rats. The lymphocyte rolling and adherence in postcapillary venules (PCV) of Peyer's patches was also significantly impaired in aged rats, with decreased expression of L-selectin on lymphocyte surfaces. Immunohistochemical analysis revealed a significant decrease in the CD8-positive cell population in Peyer's patches of the aged group, although mucosal addressin cell adhesion molecule-1 (MAdCAM-1) expression in postcapillary venules was unaltered. Lymphocyte adoptive-transfer studies indicated that although both the donor and recipient factors influence the adherence of T cells, the former may play a predominant role in the age-related change. This study clearly demonstrated in situ that T cell migration into Peyer's patches is significantly decreased in the aged intestine, which may reflect the impaired immune responses in the aging process.
authors.affiliation: Department of Internal Medicine, School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan. t-ogino@nx.catv.ne.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Ogino
authors.forename: Soichiro
authors.initials: S
authors.lastname: Miura
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Komoto
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Hara
authors.forename: Ryota
authors.initials: R
authors.lastname: Hokari
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Tsuzuki
authors.forename: Chikako
authors.initials: C
authors.lastname: Watanabe
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Koseki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nagata
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hachimura
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kaminogawa
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Ishii
mesh_terms: D019264:Adoptive Transfer; D000375:Aging / Q000276:immunology*; D000818:Animals; D002465:Cell Movement / Q000276:immunology*; D004727:Endothelium / Q000378:metabolism; D007136:Immunoglobulins / Q000378:metabolism; D039441:Integrin alpha4 / Q000378:metabolism; D007421:Intestine, Small / Q000276:immunology; D019041:L-Selectin / Q000378:metabolism; D008297:Male; D009088:Mucoproteins / Q000378:metabolism; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology*; D051381:Rats; D011916:Rats, Inbred F344; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000378:metabolism
chemical_list: D007136:Immunoglobulins; C504268:Madcam1 protein, rat; D009088:Mucoproteins; D019041:L-Selectin; D039441:Integrin alpha4