Preliminary clinical evaluation of a protein chip for tumor marker serodiagnosis of various cancers.
This preliminary study aimed to investigate sensitivity and specificity of a protein chip system for multi-tumor marker serodiagnosis of ten types of cancers, and to understand the possible clinical applications of this protein chip for the Thai population. The specific cancers diagnosed by this protein chip are lung, breast, liver, cervix, colo-rectal, stomach, ovary, esophagus, prostate and pancreas cancers. We analyzed 215 serum samples of which 165 were obtained from clinically confirmed cancer patients and 50 from healthy people with no evidence of cancer. The sensitivity and specificity of the protein chip were 82.4% and 94.0%, respectively. The success rate of the protein chip for detecting all 10 types of cancers varied from 57% to 100%. The value of the simultaneous measurement of multiple tumor markers using the protein chip for cancer screening lied in the higher sensitivity compared to using single tumor markers for each type of cancer. In short, protein chips may be useful in mass screening for cancer during health checkups as well as for metastasis follow-up of cancer patients.
Balachandra
Kruavon
K
National Institute of Health, Department of Medical Sciences, Siriraj Hospital, Mahidol University, Thailand.
Laisupasin
Pikul
P
Dhepakson
Panadda
P
Warachit
Jiranan
J
Jantraraksri
Udom
U
Issaragrisil
Surapol
S
Yang
Xiao-Li
XL
Hus
Geng-Xi
GX
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood*; D002272:Carcinoembryonic Antigen / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000097:blood; D009369:Neoplasms / Q000097:blood* / Q000175:diagnosis*; D040081:Protein Array Analysis; D012698:Serologic Tests; D013785:Thailand; D000509:alpha-Fetoproteins / Q000378:metabolism
D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D009363:Neoplasm Proteins; D000509:alpha-Fetoproteins