HPLC-radiometric determination of quinlukast in biological fluids.
An analytical method for the analysis of a novel antiasthmatic drug quinlukast and its metabolites in the plasma, bile and urine was developed. For the analysis, the solid phase extraction method and the C(8) RP-HPLC with radiometric detection of the drug were used. This method enables a quantitative determination of the agent and all of its metabolites (even of those with an unknown structure) in a biological system. The procedure is, therefore, suitable both for the pharmacokinetic analysis of quinlukast and the determination of its elimination pathways.
Syrovatko
Matej
M
Faculty of Pharmacy, Charles University with the Research Centre, Heyrovskeho 1203, CZ-500 05 Hradec Kralove, Czech Republic. syrovatko@faf.cuni.cz
Laznickova
Alice
A
Laznicek
Milan
M
Wsol
Vladimir
V
D000818:Animals; D018927:Anti-Asthmatic Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D001646:Bile / Q000737:chemistry; D001826:Body Fluids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D007275:Injections, Intravenous; D008297:Male; D015394:Molecular Structure; D011804:Quinolines / Q000032:analysis* / Q000097:blood / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014316:Tritium
D018927:Anti-Asthmatic Agents; D011804:Quinolines; C475541:VUFB19363; D014316:Tritium