Effects of methanolic extract and fractions from Litsea cubeba bark on the production of inflammatory mediators in RAW264.7 cells.
Both the methanolic extract and its fractions (0.01 mg/ml) from Litsea cubeba bark were found to inhibit NO and PGE2 production in LPS-activated RAW264.7 macrophages without significant cytotoxicity at less than 0.01 mg/ml concentration. Moreover, L. cubeba bark extract and its fractions significantly decreased the activity of myeloperoxidase catalyzing oxidation of chloride to HOCl and O2- production was reduced by methanol extract (0.05 mg/ml), chloroform fraction (0.025-0.05 mg/ml) and butanol fraction (0.005 mg/ml). These findings suggest that L. cubeba is beneficial for inflammatory conditions and may contain compound(s) with anti-inflammatory properties.
Choi
Eun-Mi
EM
Department of Biotechnology and Bioproducts Research Center, Yonsei University, Shinchon-dong 134, Seodaemun-gu, Seoul 120-749, South Korea.
Hwang
Jae-Kwan
JK
D000818:Animals; D002470:Cell Survival; D015232:Dinoprostone / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D008070:Lipopolysaccharides; D032862:Litsea; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis*; D010084:Oxidation-Reduction; D008517:Phytotherapy; D024301:Plant Bark; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
D016166:Free Radical Scavengers; D008070:Lipopolysaccharides; D010936:Plant Extracts; D009569:Nitric Oxide; D015232:Dinoprostone