A novel gene, Jpk, induces apoptosis in F9 murine teratocarcinoma cell through ROS generation.
A novel gene Jpk (Jopock) has been originally isolated through yeast 1 hybridization technique as a trans-acting factor interacting with the position-specific regulatory element of a murine Hoxa-7. Northern analysis revealed that the Jpk was expressed at day 7.0 post coitum (p.c.) during early gastrulation. Previously it has been shown that a trace amount of JPK protein led bacterial cells to death. In eukaryotic F9 cells, Jpk also led the cell to death-generating DNA ladder: fewer than 50% of the cells survived after 72-h transfection. Flow cytometric analysis with cells stained with each Annexin V/7-amino-actinomycin D (7-AAD), MitoTracker, and hydroethidine (HE) revealed that Jpk induced apoptotic cell death in a time-dependent manner, reduced mitochondrial membrane potential, and increased ROS (reactive oxygen species) production, respectively. Additionally, Jpk seemed to regulate the Bcl family at the transcriptional level when RT-PCR was performed. Although the precise mechanism is not clear, these results altogether suggest that Jpk is a potent inducer of apoptosis through generation of ROS as well as concomitant reduction of mitochondrial membrane potential.
Kong
Kyoung-Ah
KA
Department of Anatomy, Embryology Laboratory, Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul 120-752, Korea.
Park
Sungdo
S
Park
Hyoungwoo
H
Kim
Myoung Hee
MH
D000818:Animals; D045744:Cell Line, Tumor; D005775:Gastrula / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D007425:Intracellular Membranes / Q000502:physiology; D008564:Membrane Potentials; D051379:Mice; D008928:Mitochondria / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D018243:Teratocarcinoma
D017382:Reactive Oxygen Species