Effects of daidzein on estrogen-receptor-positive and negative pancreatic cancer cells in vitro.
OBJECTIVE
To study the effects of daidzein on human pancreatic cancer cells in vitro.


METHODS
Human estrogen-receptor (ER)-positive pancreatic cancer cells MiaPaCa-2 and ER-negative pancreatic cancer cells PANC-1 were treated by 0.1 micromol/L, 1 micromol/L, 10 microL, 25 microL, 50 microL, 75 microL and 100 microL of daidzein, respectively. Its antiproliferative effect was studied by MTT assay.


RESULTS
Daidzein inhibited the growth of MiaPaCa-2 and PANC-1 cells at the concentrations from 0.1 microL to 100 microL. A dose- and time-dependent manner was found. The IC(50) of daidzein on MiaPaCa-2 and PANC-1 cells was 45 microL and 75 micro, respectively. After MiaPaCa-2 cells were treated by daidzein for 3 d and at the concentrations more than IC(50), the inhibitory manner was identical and the inhibition appeared a saturation phenomenon, but the inhibitory manner of daidzein on PANC-1 cells was different from that of MiaPaCa-2 cells.


CONCLUSIONS
Daidzein has antiproliferative effects on human estrogen-receptor-positive and negative pancreatic cancer cells, but their mechanisms may be different.
Guo
Jun-Ming
JM
School of Medicine, Ningbo University, Ningbo 315211, Zhejiang Province, China. junmingguo@yahoo.com
Xiao
Bing-Xiu
BX
Dai
De-Jian
DJ
Liu
Qiong
Q
Ma
Hong-Hui
HH
D045744:Cell Line, Tumor; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D011960:Receptors, Estrogen / Q000378:metabolism*
D004968:Estrogens, Non-Steroidal; D007529:Isoflavones; D011960:Receptors, Estrogen; C004742:daidzein