Isolation and sequencing a genomic DNA encoding for phenylalanine ammonia-lyase from Phalaenopsis.
The enzyme L-phenylalanine ammonia-lyase is universally present in higher plants and it catalyzes the first committed reaction for a central pathway that generates hundreds of different phenylpropanoid metabolites including anthocyanin, the main pigments in flowers. In this study, we have successfully isolated and analyzed a phenylalanine ammonia-lyase gene from Phalaenopsis. The gene spans 3265bp and consists of two exons and one intron and encodes a polypeptide of 703 amino acid residues.
Su
Vincent
V
Department of Life Science, National Tsing Hua University, Hsin-Chu 30055, Taiwan.
Hsu
Ban-Dar
BD
D000595:Amino Acid Sequence; D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D029595:Orchidaceae / Q000201:enzymology* / Q000235:genetics; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018744:DNA, Plant; D010650:Phenylalanine Ammonia-Lyase