title: Elevated MnSOD is not required for exercise-induced cardioprotection against myocardial stunning.
abstract: Endurance exercise provides cardioprotection against ischemia-reperfusion-induced myocardial stunning and infarction. A recent study demonstrates that an exercise-induced increase in myocardial manganese superoxide dismutase (MnSOD) activity is essential to protect the heart against infarction. It is unknown if an elevation in cardiac MnSOD is also a prerequisite to achieve exercise-induced protection against myocardial stunning. Therefore, this study determined if an exercise-induced increase in myocardial MnSOD activity is a requirement to achieve protection against myocardial stunning. Adult male rats remained sedentary or performed successive bouts of endurance exercise. Hearts were exposed to 25 min of global ischemia followed by reperfusion in an isolated working heart preparation. Postischemic recovery of cardiac external work during reperfusion was significantly higher (84 +/- 3 vs. 67 +/- 4%) in exercised animals compared with sedentary controls. Furthermore, prevention of exercise-induced expression of myocardial MnSOD via antisense oligonucleotides did not retard this exercise-induced protection against myocardial stunning. These data demonstrate that exercise-induced increases in cardiac MnSOD activity are not essential to achieve exercise-mediated protection against myocardial stunning. Therefore, we conclude that different mediators are responsible for exercise-induced cardioprotection against myocardial stunning and infarction.
authors.affiliation: Department of Exercise and Sport Science, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Shannon L
authors.initials: SL
authors.lastname: Lennon
authors.forename: John C
authors.initials: JC
authors.lastname: Quindry
authors.forename: Karyn L
authors.initials: KL
authors.lastname: Hamilton
authors.forename: Joel P
authors.initials: JP
authors.lastname: French
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Hughes
authors.forename: Jay L
authors.initials: JL
authors.lastname: Mehta
authors.forename: Scott K
authors.initials: SK
authors.lastname: Powers
mesh_terms: D000818:Animals; D019610:Cytoprotection; D006321:Heart / Q000503:physiopathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D009043:Motor Activity / Q000502:physiology*; D017202:Myocardial Ischemia / Q000378:metabolism / Q000503:physiopathology; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology; D017682:Myocardial Stunning / Q000517:prevention & control*; D009206:Myocardium / Q000201:enzymology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase