Osteoblasts and osteoclasts express PEDF, VEGF-A isoforms, and VEGF receptors: possible mediators of angiogenesis and matrix remodeling in the bone.
Pigment epithelial derived factor (PEDF) is one of the most effective inhibitors of angiogenesis described so far, especially in controlling the growth of blood vessels in the eye. We now describe the localization of PEDF in regions of active bone formation in the mid-gestation mouse embryo and its specific and high levels of secretion by osteoblasts. PEDF is detected to a lesser extent in osteoclasts as well. The proangiogenic factors, VEGF and its receptors VEGF-R1 and VEGF-R2, are also expressed by both osteoblasts and osteoclasts. These findings suggest that bone angiogenesis and matrix remodeling may be mediated both by PEDF and by VEGF.
Tombran-Tink
J
J
Division of Pharmaceutical Sciences, University of Missouri-Kansas City, 5005 Rockhill Road, Kansas City, MO 64110, USA. Jttink@aol.com
Barnstable
C J
CJ
D000818:Animals; D001846:Bone Development; D001857:Bone Matrix / Q000737:chemistry; D016723:Bone Remodeling; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000378:metabolism; D005136:Eye Proteins; D015870:Gene Expression; D051379:Mice; D008807:Mice, Inbred BALB C; D018919:Neovascularization, Physiologic; D009414:Nerve Growth Factors; D010006:Osteoblasts / Q000378:metabolism*; D010010:Osteoclasts / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D011506:Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D040262:Receptors, Vascular Endothelial Growth Factor / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D015843:Serpins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D000099174:Pigment Epithelium-Derived Factor
D005136:Eye Proteins; D009414:Nerve Growth Factors; D020033:Protein Isoforms; D011506:Proteins; D012333:RNA, Messenger; D040262:Receptors, Vascular Endothelial Growth Factor; D015843:Serpins; D042461:Vascular Endothelial Growth Factor A; D000099174:Pigment Epithelium-Derived Factor