title: Effect of the antibacterial activity of pig pancreatic juice on human multiresistant bacteria.
abstract: BACKGROUND
  The role of the exocrine pancreas in regulating gut microflora colonization is unclear. The main objective in the current study was to assess the effect of pancreatic fluid on the growth of pathogenic bacteria and fungi.
  
  
  METHODS
  The antibacterial activity of pure pig pancreatic juice collected from catheterized, healthy, conscious, and anesthetized pigs was investigated with multiresistant microbial isolates and nonpathogenic strains. Studies were performed on pathogenic bacterial and fungi as well as lactic acid bacteria and reference strains.
  
  
  RESULTS
  Pancreatic juice was effective (P < 0.01) against multidrug resistant bacterial pathogens, whereas lactic acid bacteria were insensitive. The antibacterial action was independent of pancreatic juice proteolytic activity. The in vitro antibacterial properties of pancreatic juice last for several hours. Data suggest that broth composition may modulate the intensity of pancreatic juice antibacterial activity.
  
  
  CONCLUSIONS
  Pancreatic juice antibacterial activity may be an important factor in limiting the colonization of pathogenic bacteria in the gastrointestinal tract. We postulate that observed antibacterial activity of the pancreatic juice could play an important role as one of the factors of innate immunity.
authors.affiliation: Department of Medical Microbiology, Dermatology and Infection, Lund University, Lund, Sweden.
authors.forename: Danuta
authors.initials: D
authors.lastname: Kruszewska
authors.forename: Asa
authors.initials: A
authors.lastname: Ljungh
authors.forename: Sean O
authors.initials: SO
authors.lastname: Hynes
authors.forename: Stefan G
authors.initials: SG
authors.lastname: Pierzynowski
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D015169:Colony Count, Microbial; D003470:Culture Media; D024901:Drug Resistance, Multiple, Bacterial; D005658:Fungi / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D006801:Humans; D010189:Pancreatic Juice / Q000737:chemistry* / Q000201:enzymology; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media