Immunostaining for human herpesvirus 8 latent nuclear antigen-1 helps distinguish Kaposi sarcoma from its mimickers.
We assessed the usefulness of a mouse monoclonal antibody (13B10) against human herpesvirus 8 (HHV-8) latent nuclear antigen-1 (LNA-1) in diagnosis of Kaposi sarcoma (KS) and for distinguishing it from various mimickers by studying 50 cases of KS and 53 mimickers (angiosarcoma, 15; kaposiform hemangioendothelioma, 6; spindle cell hemangioma, 3; reactive angioendotheliomatosis, 3; bacillary angiomatosis, 4; acroangiomatous dematitis, 2; microvenular hemangioma, 2; hobnail hemangioma, 2; pyogenic granuloma, 5; dermatofibroma, 8; arteriovenous hemangioma, 1; verrucous hemangioma, 1; nonspecific vascular proliferation, 1) from patients with or without acquired HIV infection. Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded tissue sections. All 50 cases of KS were positive for HHV-8 LNA-1, with immunolocalization in the nuclei of the spindle cells and cells lining the primitive and thin-walled vascular channels, whereas all 53 mimickers (including 4 lesions from HIV-positive patients) tested negative. The results idicate that positive immunostaining for HHV-8 LNA- 1 exhibits high sensitivity and specificity for the diagnosis of KS and is, thus, useful for distinguishing it from the mimickers.
Cheuk
Wah
W
Department of Pathology, Queen Elizabeth Hospital, Kowloon, Hong Kong, SAR China.
Wong
Kathy O Y
KO
Wong
Cesar S C
CS
Dinkel
J E
JE
Ben-Dor
David
D
Chan
John K C
JK
D000328:Adult; D000911:Antibodies, Monoclonal; D003937:Diagnosis, Differential; D019288:Herpesvirus 8, Human / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000276:immunology; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000276:immunology; D012514:Sarcoma, Kaposi / Q000175:diagnosis* / Q000821:virology; D012680:Sensitivity and Specificity; D012871:Skin Diseases / Q000175:diagnosis
D000911:Antibodies, Monoclonal; D009687:Nuclear Proteins; D010750:Phosphoproteins; C118485:latent nuclear antigen (LNA)