title: [Comparison between hemolysis percentage measurement and hemiglobincyanide measurement for standardizing the evaluation of hemolytic properties of biomaterials].
abstract: In this study, two methods--the supernatant hemoglobin spectrophotometry, i.e. hemolysis percentage measurement(according to ISO/TR 7405) and the hemiglobincyanide measurement(according to ISO 10993-4)--were used to assay the hemolytic properties of hydroxyapatite(bioceramics) and collagen (polymer). The results showed that the conclusions drawn from using the two methods were basically consistent, and the latter was more sensitive, stable and comparable. However, some of the procedures in the hemiglobincyanide method were not defined in details. So based on our experiments we have offered some suggestions and improvements, which do not deviate from ISO and ASTM standards, for hiher practicability of usig it in standardizing the evaluation of the hemolytic properties of biomaterials. Hemiglobincyanide measurement is worthy of wider application.
authors.affiliation: Biological Evaluation Laboratory, Analysis and Testing Center, Engineering Reseach Center in Biomaterials, Sichuan University, Chengdu 610064, China.
authors.forename: Lingli
authors.initials: L
authors.lastname: Zhang
authors.forename: Weijing
authors.initials: W
authors.lastname: Zhu
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Tan
authors.forename: Shuxin
authors.initials: S
authors.lastname: Qu
authors.forename: Xingdong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D003094:Collagen; D017886:Durapatite; D006454:Hemoglobins; D006461:Hemolysis; D006801:Humans; D008422:Materials Testing / Q000379:methods*; D008706:Methemoglobin / Q000031:analogs & derivatives*; D011817:Rabbits
chemical_list: D006454:Hemoglobins; C018958:hemiglobincyanide; D003094:Collagen; D008706:Methemoglobin; D017886:Durapatite