title: Mass-tag technology for monitoring of protein kinase activity using mass spectrometry.
abstract: Monitoring of intracellular protein kinase activity is very important for fields involving diagnosis and drug screening. However, current methods, such as radiometry using (32)P, or ELISA, are laborious and time-consuming. We have developed high-throughput assay system of protein kinase activity using mass-tagged substrate peptide probes and mass spectrometry. This assay system can easily evaluate target kinase activity and will potentially be able to simultaneously profile many protein kinase activities.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Sonoda
authors.forename: Syuhei
authors.initials: S
authors.lastname: Shigaki
authors.forename: Takeyuki
authors.initials: T
authors.lastname: Nagashima
authors.forename: Osamu
authors.initials: O
authors.lastname: Okitsu
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kita
authors.forename: Masaharu
authors.initials: M
authors.lastname: Murata
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Katayama
mesh_terms: D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D011494:Protein Kinases / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D010455:Peptides; D011494:Protein Kinases