cAMP detection methods in HTS: selecting the best from the rest.
The number of technologies that enable high-throughput functional screening of G-protein-coupled receptors has expanded markedly over the past 5 years. Consequently, choosing the most appropriate technology can be a daunting task, particularly for Gi- or Gs-coupled receptors. The most common systems for cyclic AMP detection are reviewed, highlighting the practical and theoretical aspects that are important in their application to high-throughput screening. Current technologies can do the job, but it is likely that the future may require development of technologies that provide even greater biological information.
Williams
Christine
C
Hit Discovery Group, Pfizer Global Research and Development, Ramsgate Road, Sandwich, Kent CT13 9NJ, UK. chris_williams@sandwich.pfizer.com
D000818:Animals; D000242:Cyclic AMP / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D015195:Drug Design; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D017930:Genes, Reporter; D006801:Humans
D000242:Cyclic AMP