Dot-blot hybridization and RT-PCR detection of extra small virus (XSV) associated with white tail disease of prawn Macrobrachium rosenbergii.
The availability of specific and reliable detection methods is essential for monitoring the health status of farmed species, particularly for viral diseases. Extra small virus (XSV), a virus-like particle, is associated with Macrobrachium rosenbergii Noda virus (MrNV) in white tail disease (WTD) of M. rosenbergii. We developed 2 genome-based detection methods for the identification of XSV, namely dot-blot hybridization and a single-step RT-PCR. Detection limits were established and are ca. 2.5 pg and 5 fg of viral RNA for dot-blot hybridization and RT-PCR, respectively. Application of the methods to field samples indicated that some animals positively diagnosed with MrNV did not contain XSV, at least within the detection limit of the methodology. This raises the question of the actual role of XSV and its interactions with MrNV in WTD of M. rosenbergii.
Sri Widada
Joannes
J
Pathogens and Immunity, CNRS, Université Montpellier II, cc 080, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France. widada@crit.univ-montp2.fr
Richard
Véronique
V
Shi
Zhengli
Z
Qian
Dong
D
Bonami
Jean-Robert
JR
D000818:Animals; D017756:Aquaculture; D017931:DNA Primers; D015342:DNA Probes; D030381:Nodaviridae; D033541:Palaemonidae / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D015342:DNA Probes; D012367:RNA, Viral