title: Spatial and temporal regulation of the homeotic selector gene Antennapedia is required for the establishment of leg identity in Drosophila.
abstract: Antennapedia is one of the homeotic selector genes required for specification of segment identity in Drosophila. Dominant mutations that ectopically express Antennapedia cause transformation of antenna to leg. Loss-of-function mutations cause partial transformation of leg to antenna. Here we examine the role of Antennapedia in the establishment of leg identity in light of recent advances in our understanding of antennal development. In Antennapedia mutant clones in the leg disc, Homothorax and Distal-less are coexpressed and act via spineless to transform proximal femur to antenna. Antennapedia is negatively regulated during leg development by Distal-less, spineless, and dachshund and this reduced Antennapedia expression is needed for the proper development of distal leg elements. These findings suggest that the temporal and spatial regulation of the homeotic selector gene Antennapedia in the leg disc is necessary for normal leg development in Drosophila.
authors.affiliation: Developmental Biology Program, European Molecular Biology Laboratory, Heidelberg 69117, Germany. brightse@imcb.nus.edu.sg
authors.forename: B Starling
authors.initials: BS
authors.lastname: Emerald
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Cohen
mesh_terms: D000818:Animals; D051943:Antennapedia Homeodomain Protein; D015820:Cadherins / Q000378:metabolism; D003433:Crosses, Genetic; D004330:Drosophila / Q000196:embryology*; D029721:Drosophila Proteins / Q000378:metabolism; D005121:Extremities / Q000196:embryology*; D005785:Gene Conversion / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D007150:Immunohistochemistry; D009687:Nuclear Proteins; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D051943:Antennapedia Homeodomain Protein; C497974:Antp protein, Drosophila; D015820:Cadherins; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D009687:Nuclear Proteins; D018336:Receptors, Aryl Hydrocarbon; D014157:Transcription Factors; C117261:distal-less protein, insect; C094449:ds protein, Drosophila; C108704:hth protein, Drosophila; C112211:ss protein, Drosophila