Phenotypic characterization of a human synovial sarcoma cell line, SW982, and its response to dexamethasone.
SW982 cells are characterized by expression of inflammatory cytokine and matrix metalloproteinase (MMP) genes and by their response to dexamethasone at different cell densities. They express genes encoding interleukin (IL)-1 beta; IL-6; transforming growth factor-beta; intercellular adhesion molecule-1; cycloxygenase (COX)-2; and MMPs, including MMP-1, MMP-2, MMP-13, and MT1-MMP; tissue inhibitor of metalloproteinase-2; and a disintegrin and metalloproteinase with thrombospondin motifs-4. Expression of all the genes examined was induced with 2 ng/ml IL-1 beta at low cell density. The cells, however, failed to express tumor necrosis factor-alpha, COX-1, and MMP-9, regardless of the presence of IL-1 beta. Dexamethasone significantly reduced IL-1 beta, IL-6, COX-2, and MMP-1 expression at high cell density. The results suggest that SW982 cells are a useful tool for studying the expression of inflammatory cytokine or MMP genes.
Yamazaki
Taisuke
T
Department of Cellular and Molecular Physiology, Kohno Clinical Medicine Research Institute, 3-4-4, Kita-Shinagawa, Tokyo 140-0001, Japan.
Yokoyama
Takashi
T
Akatsu
Hiromi
H
Tukiyama
Takashi
T
Tokiwa
Takayoshi
T
D045744:Cell Line, Tumor / Q000187:drug effects* / Q000276:immunology; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D020782:Matrix Metalloproteinases / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D013584:Sarcoma, Synovial / Q000235:genetics / Q000276:immunology / Q000473:pathology*
D016207:Cytokines; D005938:Glucocorticoids; D003907:Dexamethasone; D020782:Matrix Metalloproteinases