title: Molecular characterisation of rough strains of Vibrio cholerae isolated from diarrhoeal cases in India and their comparison to smooth strains.
abstract: Sixteen of the 18 Vibrio cholerae rough strains isolated from hospitalised diarrhoea patients were found to contain O1 serotype-specific (wbe) genes and all currently known virulence genes. Expression of the regulatory element ToxR was evident in these strains. Cholera toxin production ability of the rough strains was found to be higher (c. three- to five-fold) as compared to the smooth counterparts and this was transcriptionally regulated. Strains exhibiting the rough phenotype were more amenable to the uptake of CTXphi, which led us to consider that the rough phenotype could play a role in the generation of genetic diversity among V. cholerae strains.
authors.affiliation: Division of Microbiology, National Institute of Cholera and Enteric Diseases, P33 CIT Road, Scheme XM, Kolkata 700 010, India.
authors.forename: Keya
authors.initials: K
authors.lastname: De
authors.forename: Thandavarayan
authors.initials: T
authors.lastname: Ramamurthy
authors.forename: Shah M
authors.initials: SM
authors.lastname: Faruque
authors.forename: Shinji
authors.initials: S
authors.lastname: Yamasaki
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Takeda
authors.forename: G Balakrish
authors.initials: GB
authors.lastname: Nair
authors.forename: Ranjan K
authors.initials: RK
authors.lastname: Nandy
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics / Q000502:physiology; D002771:Cholera / Q000382:microbiology*; D002772:Cholera Toxin / Q000096:biosynthesis / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D003967:Diarrhea / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D044404:Genomic Islands / Q000235:genetics; D006801:Humans; D007194:India; D008242:Lysogeny; D017720:Molecular Epidemiology; D019081:O Antigens / Q000032:analysis / Q000235:genetics; D021521:Ribotyping; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic; D014161:Transduction, Genetic; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D037521:Virulence Factors / Q000032:analysis / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D019081:O Antigens; D014157:Transcription Factors; D037521:Virulence Factors; C499324:toxR protein, Vibrio cholerae; C066719:toxR protein, bacteria; C075626:tcpN protein, Vibrio cholerae; D002772:Cholera Toxin