Phylogenetic analysis of the genera Streptomyces and Kitasatospora based on partial RNA polymerase beta-subunit gene (rpoB) sequences.
The RNA polymerase beta-subunit genes (rpoB) of 67 Streptomyces strains, representing 57 species, five Kitasatospora strains and Micromonospora echinospora KCTC 9549 were partially sequenced using a pair of rpoB PCR primers. Among the streptomycetes, 99.7-100 % similarity within the same species and 90.2-99.3 % similarity at the interspecific level were observed by analysis of the determined rpoB sequences. The topology of the phylogenetic tree based on rpoB sequences was similar to that of 16S rDNA. The five Kitasatospora strains formed a stable monophyletic clade and a sister group to the clade comprising all Streptomyces species. Although there were several discrepancies in the details, considerable agreement was found between the results of rpoB analysis and those of numerical phenetic classification. This study demonstrates that analysis of rpoB can be used as an alternative genetic method in parallel to conventional taxonomic methods, including numerical phenetic and 16S rDNA analyses, for the phylogenetic analyses of the genera Streptomyces and Kitasatospora.
Kim
Bum-Joon
BJ
Department of Microbiology, College of Medicine, Seoul National University, Seoul 110-799, Korea.
Kim
Chang-Jin
CJ
Korea Research Institute of Bioscience and Biotechnology, Yusung, Taejon 305-600, Korea.
Chun
Jongsik
J
School of Biological Sciences, Seoul National University, Seoul 151-742, Korea.
Koh
Young-Hwan
YH
Department of Food Science and Engineering, Cheju National University, Jeju 690-756, Korea.
Lee
Sueng-Hyun
SH
Department of Microbiology, College of Medicine, Konkuk University, Chungju 380-230, Korea.
Hyun
Jin-Won
JW
Department of Biochemistry, College of Medicine, Cheju National University, Jeju 690-756, Korea.
Cha
Chang-Yong
CY
Department of Microbiology, College of Medicine, Seoul National University, Seoul 110-799, Korea.
Kook
Yoon-Hoh
YH
Department of Microbiology, College of Medicine, Seoul National University, Seoul 110-799, Korea.
D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013302:Streptomyces / Q000145:classification* / Q000235:genetics; D013306:Streptomycetaceae / Q000145:classification* / Q000235:genetics
D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit