Effects of aging and advanced glycation on gene expression in cerebrum and spleen of mice.
OBJECTIVE
To analyze the effects of aging or advanced glycation on gene expression in the cerebrum and spleen of female C57BL/6J mice.


METHODS
The gene expression profile was determined by using cDNA expression arrays containing 588 cDNA.


RESULTS
Aging and advanced glycation resulted in differential gene expression patterns of cerebrum and spleen compared with young mice. Among the 80 genes detected in cerebrum, 43 exhibited a change in mRNA ratios with aging or treatment. Thirty-four changes (79%) were common in aged and D-galactose treated mice, whereas the cerebrum from aged and AGE-lysine treated mice showed common changes in expression of 38 genes (88%). Of the 86 genes detected in spleen, 29 (34%) displayed an age-related decrease in expression, whereas 3 (3%) displayed an increase in expression levels with aging. Eighteen genes from the detectable genes exhibited expression changes in both cerebrum and spleen of mice.


CONCLUSIONS
The gene expression profiles of D-galactose and AGE-lysine treated mice resemble those of aged mice. Use of cDNA hybridization arrays may provide a promising tool to explore the mechanism of aging at a molecular level.
Liang
Yue-Xin
YX
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Wang
Zhen
Z
Li
Dian-Dong
DD
Jiang
Jian-Min
JM
Shao
Rong-Guang
RG
D000375:Aging / Q000502:physiology*; D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D005947:Glucose / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D013154:Spleen / Q000502:physiology*; D013687:Telencephalon / Q000502:physiology*
D005947:Glucose