[Determination of daurisoline in rabbit plasma and study on its pharmacokinetics].
OBJECTIVE
To establish an HPLC method for the determination of daurisoline (DS) in rabbit plasma and investigate its pharmacokinetic characteristics after intravenous administration.


METHODS
Dauricine was used as internal standard. The plasma samples were deproteinated with acetonitrile and extracted with two-step dichloromethane. Acetonitrile-water-triethylamine (18:82:0.28, pH 3) was used as mobile phase at a flow rate of 1.0 mL.min-1. The UV-detector was set at 284 nm.


RESULTS
The linear range was 0.05-20.00 micrograms.h.mL-1 with correlation coefficients 0.9996. The limit of quantitation (LOQ) was 0.05 mg.L-1 of plasma. The absolute and relative recoveries were above 80% and (101 +/- 5)%, respectively. The intra- and inter-assay coefficient of variation were 1.9%-5.6% and 3.5%-6.5%, respectively. The plasma concentration-time profiles were adequately described by a two-compartment open model.


CONCLUSIONS
A sensitive, precise and accurate method for the determination of DS in plasma was described, which can be used in its pharmacokinetic studies.
Gu
Shi-fen
SF
Institute of Clinical Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Shi
Shao-jun
SJ
Chen
Hui
H
D000818:Animals; D019540:Area Under Curve; D044182:Benzylisoquinolines / Q000097:blood* / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008297:Male; D011817:Rabbits
D044182:Benzylisoquinolines; C023968:daurisoline