title: Recombinant avian adenovirus CELO expressing the human interleukin-2: characterization in vitro, in ovo and in vivo.
abstract: In our study, a recombinant adenovirus based on the avian adenovirus CELO genome, has been constructed that contains the human interleukin-2 gene. We have shown the production of biologically active recombinant interleukin-2 in vitro (LMH and 293 cells) and in ovo (chicken embryos) infected with recombinant virus CELO-IL2. An increase in the median survival time of C57BL/6 mice carrying B16 melanoma tumors has been demonstrated after multiple intra-tumors injections of the recombinant adenovirus CELO-IL2.
authors.affiliation: Gamaleya Research Institute of Epidemiology and Microbiology, Gamaleya Street 18, 123098 Moscow, Russia. cherenova@aport2000.ru
authors.forename: L V
authors.initials: LV
authors.lastname: Cherenova
authors.forename: D Y
authors.initials: DY
authors.lastname: Logunov
authors.forename: E V
authors.initials: EV
authors.lastname: Shashkova
authors.forename: M M
authors.initials: MM
authors.lastname: Shmarov
authors.forename: L V
authors.initials: LV
authors.lastname: Verkhovskaya
authors.forename: G L
authors.initials: GL
authors.lastname: Neugodova
authors.forename: D B
authors.initials: DB
authors.lastname: Kazansky
authors.forename: K K
authors.initials: KK
authors.lastname: Doronin
authors.forename: B S
authors.initials: BS
authors.lastname: Naroditsky
mesh_terms: D000818:Animals; D002642:Chick Embryo / Q000821:virology; D003001:Cloning, Molecular / Q000379:methods; D005260:Female; D025681:Fowl adenovirus A / Q000235:genetics*; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics* / Q000627:therapeutic use; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010957:Plasmids; D016019:Survival Analysis
chemical_list: D007376:Interleukin-2