title: Angiotensin converting enzyme inhibitory activity in commercial fermented products. Formation of peptides under simulated gastrointestinal digestion.
abstract: The angiotensin converting enzyme (ACE)-inhibitory activity of several commercial fermented milks was evaluated. Most of these products showed moderate inhibitory activity, but a few exceptions were detected. The high ACE-inhibitory activity found in some cases could be related to the origin of the milk. Two of these products were subjected to an enzymatic hydrolysis process, which simulates physiological digestion, to study the influence of digestion on ACE-inhibitory activity. The activity did not significantly change or increase during simulated gastrointestinal digestion. The peptides generated from one selected product during simulated digestion were sequenced by tandem spectrometry. Most peptides found at the end of the simulated digestion were released after 30 min of incubation with the pancreatic extract. This suggests that physiological digestion promotes the formation of active peptides from the proteins present in these fermented products. The potential ACE-inhibitory activity of the identified peptides is discussed with regard to their amino acid sequences.
authors.affiliation: Instituto de Fermentaciones Industriales (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: Blanca
authors.initials: B
authors.lastname: Hernández-Ledesma
authors.forename: Lourdes
authors.initials: L
authors.lastname: Amigo
authors.forename: Mercedes
authors.initials: M
authors.lastname: Ramos
authors.forename: Isidra
authors.initials: I
authors.lastname: Recio
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D043302:Cultured Milk Products / Q000737:chemistry*; D004063:Digestion; D004064:Digestive System / Q000378:metabolism*; D006868:Hydrolysis; D013058:Mass Spectrometry; D008954:Models, Biological; D010179:Pancreas / Q000737:chemistry; D010455:Peptides / Q000032:analysis / Q000378:metabolism*; D013053:Spectrophotometry; D014020:Tissue Extracts / Q000378:metabolism
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D010455:Peptides; D014020:Tissue Extracts