Mannan from Aloe saponaria inhibits tumoral cell activation and proliferation.
In this study, we tested the antiproliferative effects of mannan from Aloe saponaria using normal murine (SpMC) and human cells (PBMC) and several tumoral cell lines. Employing flow cytometry, it could be determined that mannan inhibits the proliferative response in normal and tumoral cells. Mannan affects the expression of CD3(+) SpMC indicating that mannan inhibits mainly T lymphocyte proliferative response. Also in SpMC cultured with or without mitogen mannan produces an increase of an activation marker (CD25). On C1498 cell line, mannan reduces CD3 expression and abolishes the CD25 expression. In conclusion, mannan has a dual beneficial effect when applied to normal and tumoral cells at the same time by inhibiting the activation of cancer cells and improving that of normal ones.
Sampedro
M Cecilia
MC
Departamento de Química Biológica-CIQUIBIC, Facultad Ciencias Químicas, Universidad Nacional de Córdoba, Cuidad Universitaria, C5000GYA-Córdoba 5016, Argentina.
Artola
Rodolfo L
RL
Murature
Mariano
M
Murature
Domingo
D
Ditamo
Yanina
Y
Roth
German A
GA
Kivatinitz
Silvia
S
D000504:Aloe / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006224:Cricetinae; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D008297:Male; D008351:Mannans / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D013154:Spleen / Q000166:cytology / Q000187:drug effects
D000970:Antineoplastic Agents; D008351:Mannans; D010936:Plant Extracts