Structural characterization of a new lipid/DNA complex showing a selective transfection efficiency in ovarian cancer cells.
We investigated, for the first time, by using Energy Dispersive X-ray Diffraction, the structure of a new ternary cationic liposome formulated with dioleoyl trimethylammonium propane (DOTAP), 1,2-dioleoyl-3-phosphatidylethanolamine (DOPE) and cholesterol (Chol) (DDC) which has been recently found to have a selective high gene transfer ability in ovarian cancer cells. Our structural results provide a further experimental support to the widely accepted statement that there is not a simple and direct correlation between structure and transfection efficiency and that the factors controlling cationic lipid/DNA (CL-DNA) complexes-mediated gene transfer depend not only on the formulations of the cationic liposomes and their thermodynamic phase, but also significantly on the cell properties.
Caracciolo
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Dipartimento di Fisica and INFM, Università La Sapienza, P le A Moro 5, 00185 Roma, Italy.
Pozzi
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Caminiti
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R
Congiu Castellano
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A
D000818:Animals; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry*; D005260:Female; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D046911:Macromolecular Substances; D008422:Materials Testing / Q000379:methods*; D008958:Models, Molecular; D008968:Molecular Conformation; D010051:Ovarian Neoplasms / Q000235:genetics / Q000628:therapy; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D014162:Transfection / Q000379:methods; D014961:X-Ray Diffraction / Q000379:methods*
C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D020099:Coated Materials, Biocompatible; D004337:Drug Carriers; D005229:Fatty Acids, Monounsaturated; D008051:Lipid Bilayers; D008081:Liposomes; D046911:Macromolecular Substances; D010714:Phosphatidylethanolamines; D000644:Quaternary Ammonium Compounds; D004247:DNA; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane