Cell growth on the porous sponges prepared from poly(depsipeptide-co-lactide) having various functional groups.
In tissue engineering, excellent biodegradable materials are desired as temporary scaffolds to support cell growth and disappear with the progress of tissue regeneration. We previously synthesized biodegradable poly(depsipeptide-co-lactide), poly[(Glc-Asp)-co-LA] and poly[(Glc-Lys)-co-LA], having reactive side-chain groups. Then, the effects of reactive and ionic side-chain groups on cell attachment and growth were investigated using co-polymer films with various amounts of carboxyl or amino groups. In this study, to evaluate the utility of these co-polymers as functional scaffolds for tissue regeneration, 3-dimensional porous sponges were prepared by freeze-drying method and the effects of reactive and ionic side-chain groups on cell growth and degradation behavior were investigated using co-polymer sponges with various amounts of carboxyl or amino groups. Good cell growth was observed on the co-polymer sponges. During cell culture, the co-polymer sponges exhibited various degradation rates related to the depsipeptide unit content. Three-dimensional biodegradable polymer matrices with reactive surface, controllable degradation behavior and good cell growth were successfully prepared using these co-polymers. Such kinds of co-polymer matrices are good candidate for scaffold for tissue engineering.
Ohya
Yuichi
Y
Department of Applied Chemistry, Faculty of Engineering & High Technology Research Center, Kansai University, Suita, Osaka 564-8680, Japan. yohya@ipcku.kansai-u.ac.jp
Matsunami
Hideaki
H
Ouchi
Tatsuro
T
D020341:Absorbable Implants; D000818:Animals; D001224:Aspartic Acid / Q000737:chemistry; D001673:Biodegradation, Environmental; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D047630:Depsipeptides; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D006016:Glycolates / Q000737:chemistry; D008239:Lysine / Q000737:chemistry; D051379:Mice; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D016062:Porosity; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods
D047630:Depsipeptides; D006016:Glycolates; D010456:Peptides, Cyclic; D011091:Polyesters; D011108:Polymers; C476738:poly(depsipeptide-co-lactide); C031149:glycolic acid; D001224:Aspartic Acid; C033616:poly(lactide); D008239:Lysine