A WT1 expressing metastatic human Kaposi sarcoma xenograft model.
We have established a non-metastatic and a metastatic human Kaposi sarcoma (KS) xenograft model in SCID mice by injecting KS-Imm cells subcutaneously and intrasplenically, respectively. KS-Imm cells expressed endothelial markers, CD34 and vWF in vivo. Furthermore, we have shown that these cells express all the splice variants of the WT1 gene and WT1wt protein in vitro and in vivo detected by nested PCR and immunohistochemistry. WT1 expression in the peripheral blood was only detectable in case of metastatic KS model suggesting it as a molecular marker of progression.
Rásó
Erzsébet
E
Department of Tumor Progression, National Institute of Oncology, Budapest, Hungary.
Mészáros
Lívia
L
Albini
Adriana
A
Tímár
József
J
D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D015415:Biomarkers; D018450:Disease Progression; D006801:Humans; D007124:Immunoenzyme Techniques; D008113:Liver Neoplasms / Q000378:metabolism* / Q000556:secondary*; D051379:Mice; D016513:Mice, SCID; D016133:Polymerase Chain Reaction; D012514:Sarcoma, Kaposi / Q000378:metabolism* / Q000473:pathology; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D025721:WT1 Proteins / Q000235:genetics* / Q000378:metabolism
D018952:Antigens, CD34; D015415:Biomarkers; D025721:WT1 Proteins