[Study on the metabolite of stilbene glucoside in mice by liquid chromatography tandem-mass spectrometry].
OBJECTIVE
To study the metabolite of stilbene glucoside in the Chinese traditional medicine Polygonum multiflornm in mice and elucidate its chemical structure by liquid chromatography tandem-mass spectrometry.


METHODS
The stilbene glucoside was injected into the tail vein of mice. Blood samples were collected from artery in the eyepit. The methanol-protein-precipitated plasma sample was introduced into the liquid chromatography-tandem mass spectrometer directly. The analytical column was C18 column (250 mm x 4.6 mm ID, 5 microns). The mobile phase consisted of acetonitrile-methanol-water-formic acid (15:18:66:1) for ES+, acetonitrile-methanol-water (15:18:67) for ES-. The UV detection wavelength was set at 320 nm. The mass ion source type was ESI. HV capillary was 3 kV. The dry gas was nitrogen gas and the flow rate was set at 318 L.h-1. The ion source temperature was 150 degrees C.


RESULTS
The stilbene glucoside and its metabolite were separated completely under the chromatography condition. The ions at m/z 600 and m/z 605 were detected under positive ion polarity while the ions at m/z 581 and m/z 402 were detected under negative ion polarity.


CONCLUSIONS
It was proposed that the metabolite of stilbene glucoside injected in vein was its glucuronide conjugate.
Sun
Jiang-hao
JH
Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China.
Zhang
Lan-tong
LT
Wang
Chun-ying
CY
Yuan
Zhi-fang
ZF
Lu
Xing-hong
XH
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D005960:Glucosides / Q000097:blood* / Q000302:isolation & purification / Q000378:metabolism; D008297:Male; D051379:Mice; D010946:Plants, Medicinal / Q000737:chemistry; D028502:Polygonum / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013267:Stilbenes / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
C489707:2',3',4',5'-tetrahydroxystilbene-2-O-beta-D-glucoside; D005960:Glucosides; D013267:Stilbenes