title: Preferential HLA usage in the influenza virus-specific CTL response.
abstract: To study whether individual HLA class I alleles are used preferentially or equally in human virus-specific CTL responses, the contribution of individual HLA-A and -B alleles to the human influenza virus-specific CTL response was investigated. To this end, PBMC were obtained from three groups of HLA-A and -B identical blood donors and stimulated with influenza virus. In the virus-specific CD8(+) T cell population, the proportion of IFN-gamma- and TNF-alpha-producing cells, restricted by individual HLA-A and -B alleles, was determined using virus-infected C1R cells expressing a single HLA-A or -B allele for restimulation of these cells. In HLA-B*2705- and HLA-B*3501-positive individuals, these alleles were preferentially used in the influenza A virus-specific CTL response, while the contribution of HLA-B*0801 and HLA-A*0101 was minor in these donors. The magnitude of the HLA-B*0801-restricted response was even lower in the presence of HLA-B*2705. C1R cells expressing HLA-B*2705, HLA-A*0101, or HLA-A*0201 were preferentially lysed by virus-specific CD8(+) T cells. In contrast, the CTL response to influenza B virus was mainly directed toward HLA-B*0801-restricted epitopes. Thus, the preferential use of HLA alleles depended on the virus studied.
authors.affiliation: Department of Virology and World Health Organization National Influenza Center, Erasmus Medical Center, Rotterdam, The Netherlands.
authors.forename: Adrianus C M
authors.initials: AC
authors.lastname: Boon
authors.forename: Gerrie
authors.initials: G
authors.lastname: De Mutsert
authors.forename: Ron A M
authors.initials: RA
authors.lastname: Fouchier
authors.forename: Kees
authors.initials: K
authors.lastname: Sintnicolaas
authors.forename: Albert D M E
authors.initials: AD
authors.lastname: Osterhaus
authors.forename: Guus F
authors.initials: GF
authors.lastname: Rimmelzwaan
mesh_terms: D000328:Adult; D000483:Alleles; D002461:Cell Line, Transformed; D003601:Cytotoxicity Tests, Immunologic / Q000706:statistics & numerical data; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D015234:HLA-A Antigens / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D015235:HLA-B Antigens / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D015796:HLA-B27 Antigen; D015797:HLA-B35 Antigen / Q000235:genetics; D006801:Humans; D009980:Influenza A virus / Q000276:immunology*; D009981:Influenza B virus / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis; D008875:Middle Aged; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism / Q000821:virology*; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D018984:Epitopes, T-Lymphocyte; D015234:HLA-A Antigens; D015235:HLA-B Antigens; C464936:HLA-B*27:05 antigen; D015796:HLA-B27 Antigen; D015797:HLA-B35 Antigen; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma