[Effects of Angelica sinensis polysaccharides on hepatic drug metabolism enzymes activities in mice].
OBJECTIVE
To study the effects of Angelica sinensis Polysaccharides (ASP) on the hepatic drug metabolism enzymes activities in normal mice and those prednisolone (PSL)-induced liver injury.


METHODS
The activities of phase II enzymes (GSH-related enzymes) and cytochrome P450 enzymes were measured by biochemical method.


RESULTS
ASP increased the activities of glutathione S-transferase in liver microsomes and mitochondria. The cytochrome P450 content, NADPH-cytochrome c reductase, aminopyrine N-demethylase, and aniline hydroxylase activities in liver microsomes were also increased. PSL significantly increased serum ALT levels, and decreased the liver mitochondrial glutathione content. At the same time, other enzymes activities were all increased. When mice were treated with ASP 2.0 g.kg-1, the PSL-induced changes on cytochrome P450 enzymes, glutathione S-transferase, and GSH content were restored.


CONCLUSIONS
ASP can modulate the activities of drug metabolism enzymes.
Xia
Xue-yan
XY
Department of Pharmacology, School of Medicine, Wuhan University, Wuhan 430071, HuBei, China.
Peng
Ren-xiu
RX
Kong
Rui
R
Yang
Zhe-qiong
ZQ
Chen
Xiao
X
D000633:Aminopyrine N-Demethylase / Q000378:metabolism; D029971:Angelica sinensis / Q000737:chemistry*; D000815:Aniline Hydroxylase / Q000378:metabolism; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology* / Q000209:etiology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D011239:Prednisolone
D011134:Polysaccharides; D003577:Cytochrome P-450 Enzyme System; D011239:Prednisolone; D000815:Aniline Hydroxylase; D000633:Aminopyrine N-Demethylase; D009251:NADPH-Ferrihemoprotein Reductase; D005982:Glutathione Transferase