[Characterization of Notch gene involved in genetic regulatory networks in dental pulp stem cells].
OBJECTIVE
To investigate the characterization of Notch gene involved in genetic regulatory networks in dental pulp stem cells.


METHODS
The pulp tissue was separated from mouse teeth and digested by collagenase type I. Single-cell suspensions of dental pulp were seeded into 6-well plates with alpha modification of Eagle's medium supplemented with ES cell qualified Fetal Bovine Serum. Colony-forming efficiency was assessed in 14ds culture. Transcripts for Notch were detected by reverse transcription-PCR by using total RNA isolated from cells.


RESULTS
There were clonogenic cells in dental pulp cell and the incidence of colony-forming cells derived from mouse dental pulp cells was 1.6-2.5 colonies/10(4) plate. Mouse-specific Notch mRNA expressed in colony-forming cells.


CONCLUSIONS
Notch mRNA expressing in colony-forming cells provided a more detailed understanding of mouse dental pulp stem cell biology.
Lu
Qun
Q
Dept. of Oral Medicine, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
Wu
Bu-ling
BL
Wang
Ji-shu
JS
Han
Hua
H
Zhou
Xue-dong
XD
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology* / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000235:genetics*; D051880:Receptors, Notch; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013234:Stem Cells / Q000378:metabolism*
D008565:Membrane Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D051880:Receptors, Notch