Molecular cloning and characterization of a novel rat CXC chemokine, rPBP, the homologue of human and mouse PBP.
We have previously cloned the mouse platelet basic protein (mPBP), a homologue of human PBP, from mouse thymic stromal cells. Using EST alignment and RT-PCR, the rat homologue of human and mouse PBP was cloned from lung and named as rPBP. The complete open reading frame and part of the 3'- and 5'-non-coding regions were obtained through rapid amplification of cDNA ends. The rPBP cDNA encodes a protein of 111 amino acids containing a signal peptide of 37 amino acids at the N-terminus, with the mature protein of 74 amino acids. The rPBP is a new member of ELR+CXC chemokines. The mature protein of rPBP shares 69% and 45% homology with mouse and human PBP, respectively. In situ hybridization assay revealed rPBP to be predominantly localized in the pulmonary vascular endothelial cells. The eukaryotic expression vector pCDNA3-rPBP was constructed and transiently transfected into COS-7 cells. In the in vitro chemotaxis assay, the polymorphonuclear leukocytes (PMNs) were chemoattracted to the supernatants from transfected COS-7 cells in a dose-dependent manner. The implication of rPBP found in rat lung is that this chemokine may have the function to recruit PMNs to fight against pulmonary infection.
Fu
Wen-Xian
WX
Department of Immunology, Peking University Health Science Center, Beijing 100083, China.
Zhu
Meng-Lei
ML
Gong
Shun-You
SY
Li
Yan
Y
Chen
Wei-Feng
WF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D019743:Chemokines, CXC / Q000737:chemistry / Q000235:genetics*; D002634:Chemotaxis, Leukocyte; D002522:Chlorocebus aethiops; D033481:Chromosomes, Mammalian / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D008168:Lung / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D016415:Sequence Alignment; D017385:Sequence Homology
D019743:Chemokines, CXC; D018076:DNA, Complementary; C493799:Ppbp protein, rat; D012333:RNA, Messenger