MUC1 can interact with adenomatous polyposis coli in breast cancer.
The MUC1 tumor antigen is overexpressed on most breast tumors and metastases. It interacts with signaling proteins such as the ErbB kinases and beta-catenin, and is involved in mammary gland oncogenesis and tumor progression. Herein, we report a novel interaction between MUC1 and adenomatous polyposis coli (APC), a tumor suppressor involved in downregulating beta-catenin signaling. Initially identified in colorectal cancer, APC is also downregulated in breast tumors and presumably involved in mammary carcinogenesis. MUC1 and APC co-immunoprecipitate from the ZR-75-1 human breast carcinoma cell line and co-localize in mouse mammary glands and tumors. These studies also indicate that the association of MUC1 and APC may be increased by epidermal growth factor stimulation. Intriguingly, the co-immunoprecipitation of MUC1 and APC increases in human breast tumors and metastases as compared to adjacent normal tissues, indicating that this association may play a role in the formation and progression of breast tumors.
Hattrup
Christine L
CL
Tumor Biology Program, Mayo Clinic College of Medicine, Mayo Clinic, Scottsdale, AZ, USA.
Fernandez-Rodriguez
Julia
J
Schroeder
Joyce A
JA
Hansson
Gunnar C
GC
Gendler
Sandra J
SJ
D025601:Adenomatous Polyposis Coli Protein / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D006801:Humans; D042361:Mammary Glands, Human / Q000737:chemistry; D015674:Mammary Neoplasms, Animal / Q000378:metabolism; D051379:Mice; D018396:Mucin-1 / Q000032:analysis / Q000378:metabolism*; D009362:Neoplasm Metastasis; D011233:Precipitin Tests
D025601:Adenomatous Polyposis Coli Protein; D018396:Mucin-1; D004815:Epidermal Growth Factor