[Use of detergents for elimination of non-specific reactions of monoclonal antibodies].
The purpose of the case study was elaboration of methods for removing the false-positive results in the detection of virus antigens that cause acute respiratory lesions both in the plane and dot-membrane variations of immune-enzyme assays of monoclonal antibodies. Modeled experiments showed that the non-specific staining of biological samples, in which the above viral antigen are looked for, is preconditioned by a direct reaction of the substrate with its own oxidizing enzymes and by the binding of monoclonal antibodies with biological macromolecules free of any viral adherence and sorbated in the solid phase. Approaches to solving such issues are described in the paper. They comprise the inhibition of the own oxidizing activity of wash-outs by the substrate and the application of detergents. Different detergents were comparatively analyzed and the optimal compounds were chosen for each variation of immune-enzyme test-systems. The described techniques ensure more reliable results in the viral antigen detection by immune-enzyme assay.
Ivanova
I A
IA
Monaenkov
A O
AO
Peshkova
V G
VG
Medvedeva
N A
NA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D003429:Cross Reactions; D003902:Detergents / Q000737:chemistry*; D006367:HeLa Cells; D006389:Hemagglutinins, Viral / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D009980:Influenza A virus / Q000276:immunology; D009981:Influenza B virus / Q000276:immunology; D007252:Influenza Vaccines / Q000032:analysis; D051379:Mice; D011136:Polysorbates / Q000737:chemistry
D000911:Antibodies, Monoclonal; D003902:Detergents; D006389:Hemagglutinins, Viral; D007252:Influenza Vaccines; D011136:Polysorbates