Characterization of [(3)H]harmane binding to rat whole brain membranes.
This study investigates the binding of [(3)H]harmane to rat whole brain homogenates. Saturation studies revealed [(3)H]harmane labels a single, saturable, high-capacity population with high affinity. All the test compounds displaced [(3)H]harmane completely and in an apparently monophasic manner. The displacement profile of the test ligands indicated labeling of MAO-A. Given the high level of MAO-A binding, it is unlikely that a low-capacity I(2) site would be distinguishable from the total [(3)H]harmane population.
Anderson
N J
NJ
Psychopharmacology Unit, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK.
Robinson
E S J
ES
Husbands
S M
SM
Delagrange
P
P
Nutt
D J
DJ
Hudson
A L
AL
D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D001923:Brain Chemistry; D002462:Cell Membrane / Q000378:metabolism*; D006247:Harmine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism*; D011869:Radioligand Assay; D051381:Rats; D014316:Tritium / Q000737:chemistry / Q000378:metabolism
D014316:Tritium; D006247:Harmine; C005010:harman