title: Fixed-time artificial insemination of postpartum beef cows at 72 or 80 h after treatment with the MGA Select protocol.
abstract: The objective was to determine the appropriate timing of fixed-time artificial insemination (AI) following administration of the MGA Select protocol. Cows at two locations (Location 1, n=114; Location 2, n=97 ) were assigned to fixed-time AI at 72 or 80 h by age, body condition score (BCS), days postpartum (DPP), AI technician, and sire. All cows were synchronized with the MGA Select protocol, consisting of oral administration of melengestrol acetate (MGA; 0.5mg/hd per day) for 14 days, GnRH (Cysotrelin, 100 microg, i.m.; Day 26) 12 days after MGA withdrawal, followed in 7 days with PGF(2alpha) (PG; Lutalyse, 25mg i.m.; Day 33). Cows were inseminated at 72 h ( n=108 ) or 80 h ( n=103 ) after PG and GnRH (100 microg) was given at insemination. Location was not significant and, therefore, was removed from the model. Mean BCS ( 5.2+/-0.1, 72 h; 5.3+/-0.1, 80 h) and DPP ( 34+/-2, 72 h; 35+/-2, 80 h) did not differ ( P>0.1 ) between treatments. Serum progesterone concentrations 7 and 1 day prior to MGA were used to determine pre-treatment cyclicity: cows with at least one sample with progesterone > or =1 ng/ml were defined as cyclic (33/108, 31%, 72 h, versus 32/103, 31%, 80 h; P>0.1). Cows with serum progesterone concentrations > or =1 ng/ml on the day of PG were defined as responding to the synchronization protocol (74/108 (69%), 72 h versus 69/103 (67%), 80 h; P>0.1 ). Although pregnancy rates were higher ( P<0.05 ) for cows inseminated at 72 h (69/108, 64%) versus 80 h (52/103, 50%) after PG, pregnancy rates at the end of the breeding season did not differ ( P>0.1 ) between treatments (98/108 (91%), 72 h; 88/103 (85%), 80 h). In conclusion, pregnancy rates were higher when postpartum beef cows synchronized with the MGA Select protocol were inseminated at 72 h versus 80 h after PG.
authors.affiliation: Department of Animal Science, University of Missouri, S132 ASRC, Columbia, MO 65211, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Stegner
authors.forename: J F
authors.initials: JF
authors.lastname: Bader
authors.forename: F N
authors.initials: FN
authors.lastname: Kojima
authors.forename: M R
authors.initials: MR
authors.lastname: Ellersieck
authors.forename: M F
authors.initials: MF
authors.lastname: Smith
authors.forename: D J
authors.initials: DJ
authors.lastname: Patterson
mesh_terms: D000818:Animals; D001947:Breeding; D002417:Cattle / Q000502:physiology*; D015237:Dinoprost / Q000008:administration & dosage*; D004973:Estrus Synchronization / Q000379:methods; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage*; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary*; D008552:Melengestrol Acetate / Q000008:administration & dosage*; D049590:Postpartum Period; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D013997:Time Factors
chemical_list: D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D008552:Melengestrol Acetate; D015237:Dinoprost