title: Altered taste function in mice deficient in the 65-kDa isoform of glutamate decarboxylase.
abstract: The 65-kDa isoform of glutamate decarboxylase (GAD65) is considered to play an important role for GABA synthesis in the central nervous system. Using mice with targeted ablation of the GAD65 gene (GAD65(-/-) mice) we investigated a possible involvement of GABAergic neurotransmission in several taste functions. Preference/aversion responses to four basic tastes were not different between GAD65(-/-) and wild-type mice during a 48-h two-bottle choice test. GAD65(-/-) mice consumed less sucrose-quinine mixtures than did wild-type mice. The injection of midazolam (5 mg/kg), a benzodiazepine agonist, significantly increased the consumption of 100 mM sucrose in the wild-type mice. The same injection, however, failed to increase intake of the 100 mM sucrose in GAD65(-/-) mice. These results suggest that GAD65-generated GABA is not implicated in basic taste functions such as simple detection and discrimination. Rather, more complex processing of taste information including taste mixtures and palatability may be finely tuned by GAD65-mediated GABA synthesis.
authors.affiliation: Department of Behavioral Physiology, Graduate School of Human Sciences, Osaka University, 1-2 Yamadaoka, Suita, Osaka 565-0871, Japan. shimura@hus.osaka-u.ac.jp
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Shimura
authors.forename: Uno
authors.initials: U
authors.lastname: Watanabe
authors.forename: Yuchio
authors.initials: Y
authors.lastname: Yanagawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamamoto
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects / Q000502:physiology; D001522:Behavior, Animal; D002755:Choice Behavior / Q000187:drug effects / Q000502:physiology; D004192:Discrimination, Psychological / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004327:Drinking Behavior / Q000187:drug effects / Q000502:physiology; D004408:Dysgeusia / Q000235:genetics*; D005260:Female; D018757:GABA Modulators / Q000494:pharmacology; D005968:Glutamate Decarboxylase / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D007527:Isoenzymes / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D008874:Midazolam / Q000494:pharmacology; D013395:Sucrose / Q000378:metabolism / Q000494:pharmacology; D013649:Taste / Q000187:drug effects / Q000235:genetics*
chemical_list: D018757:GABA Modulators; D007527:Isoenzymes; D013395:Sucrose; D005968:Glutamate Decarboxylase; C401141:glutamate decarboxylase 2; D008874:Midazolam