title: Evaluation and comparison of tailor-made stationary phases based on spherical silica-based beads for capillary electrochromatography via peptide separation analysis.
abstract: Small cyclic peptides have been employed to elucidate the performance of novel sorbents as stationary phases in capillary electrochromatography (CEC). In this paper chain length dependencies for ordinary liquid chromatographic sorbents are reported together with findings acquired on beads specifically designed to suit CEC. The latter, tailor-made, spherical, porous silica exhibits a distinguished surface modification to meet the criteria anticipated to enhance performance profiles in CEC. With well-characterised peptides resembling the analytes, probing of the CEC system in a systematic manner (predominantly via the organic modifier content of the background electrolyte (BE)) reveals insight into the complex interplay occurring in such analytical systems at the molecular and sub-molecular level in particular upon various modes of interaction.
authors.affiliation: Institut für Anorganische Chemie und Analytische Chemie, Johannes Gutenberg-Universität, Duesbergweg 10-14, D-55099 Mainz, Germany.
authors.forename: M I
authors.initials: MI
authors.lastname: Huber
authors.forename: T P
authors.initials: TP
authors.lastname: Hennessy
authors.forename: Dieter
authors.initials: D
authors.lastname: Lubda
authors.forename: K K
authors.initials: KK
authors.lastname: Unger
mesh_terms: D000595:Amino Acid Sequence; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation* / Q000379:methods; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D012822:Silicon Dioxide
chemical_list: D010455:Peptides; D012822:Silicon Dioxide