Nonsense-mediated mRNA decay: splicing, translation and mRNP dynamics.
Studies of nonsense-mediated mRNA decay in mammalian cells have proffered unforeseen insights into changes in mRNA-protein interactions throughout the lifetime of an mRNA. Remarkably, mRNA acquires a complex of proteins at each exon-exon junction during pre-mRNA splicing that influences the subsequent steps of mRNA translation and nonsense-mediated mRNA decay. Complex-loaded mRNA is thought to undergo a pioneer round of translation when still bound by cap-binding proteins CBP80 and CBP20 and poly(A)-binding protein 2. The acquisition and loss of mRNA-associated proteins accompanies the transition from the pioneer round to subsequent rounds of translation, and from translational competence to substrate for nonsense-mediated mRNA decay.
Maquat
Lynne E
LE
Department of Biochemistry and Biophysics, School of Medicine and Dentistry, 601 Elmwood Avenue, Box 712, University of Rochester, Rochester, New York 14642, USA. lynne_maquat@urmc.rochester.edu
D000818:Animals; D018389:Codon, Nonsense; D005091:Exons; D005656:Fungal Proteins / Q000378:metabolism; D008957:Models, Genetic; D014176:Protein Biosynthesis; D012315:RNA Caps; D012322:RNA Precursors / Q000378:metabolism*; D012326:RNA Splicing; D012333:RNA, Messenger / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism*
D018389:Codon, Nonsense; D005656:Fungal Proteins; D012315:RNA Caps; D012322:RNA Precursors; D012333:RNA, Messenger; D012261:Ribonucleoproteins; C026452:messenger ribonucleoprotein