Evidence of caspase-3 activation in hyposmotic stress-induced necrosis.
Primary culture of dentate gyrus was submitted to a hyposmotic stress that induces a rapid cell death that is necrosis morphologically. Surprisingly, we observed a rapid and dramatic upregulation of the active form of caspase-3 (caspase-3(a)) in both neurons and glial cells. Caspase-3(a) immunoreactivity appears as early as 1 min after hyposmotic treatment, when some neurons are still alive, suggesting that caspase-3(a) may contribute to further necrotic cell death.
Niquet
Jerome
J
Epilepsy Research, Research 151, Veterans Affairs Greater Los Angeles Healthcare System, 11301 Wilshire Boulevard, West Los Angeles, CA 90073, USA. jniquet@ucla.edu
Allen
Suni G
SG
Baldwin
Roger A
RA
Wasterlain
Claude G
CG
D000818:Animals; D000831:Animals, Newborn; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism*; D002478:Cells, Cultured; D018891:Dentate Gyrus / Q000201:enzymology / Q000473:pathology* / Q000648:ultrastructure; D004789:Enzyme Activation; D007150:Immunohistochemistry / Q000379:methods; D008854:Microscopy, Electron / Q000379:methods; D009336:Necrosis; D009457:Neuroglia / Q000473:pathology / Q000648:ultrastructure; D009474:Neurons / Q000201:enzymology / Q000473:pathology / Q000648:ultrastructure; D009994:Osmolar Concentration; D011419:Propidium / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological / Q000201:enzymology* / Q000503:physiopathology; D014867:Water / Q000009:adverse effects
D014867:Water; D011419:Propidium; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases