title: Sequence determination of the Crimean-Congo hemorrhagic fever virus L segment.
abstract: Crimean-Congo hemorrhagic fever (CCHF) virus is highly pathogenic for humans and remains the only Category A virus for which full sequence information is currently unavailable. In this study we completed CCHF genome characterization by determining the L segment sequence using Dugbe and CCHF virus-specific oligonucleotides. Sequence alignments revealed the presence of four previously described conserved regions in all Bunyaviridae polymerases. Interestingly, additional regions containing putative Ovarian Tumor (OTU)-like cysteine protease and helicase domains were identified in the L segments of CCHF and Dugbe viruses, suggesting an autoproteolytic cleavage process for nairovirus L proteins.
authors.affiliation: Special Pathogens Program, National Microbiology Laboratory, Health Canada, Canadian Science Centre for Human and Animal Health, Winnipeg, Canada R3E 3R2.
authors.forename: Erin
authors.initials: E
authors.lastname: Kinsella
authors.forename: Sandra G
authors.initials: SG
authors.lastname: Martin
authors.forename: Allen
authors.initials: A
authors.lastname: Grolla
authors.forename: Markus
authors.initials: M
authors.lastname: Czub
authors.forename: Heinz
authors.initials: H
authors.lastname: Feldmann
authors.forename: Ramon
authors.initials: R
authors.lastname: Flick
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000235:genetics; D016679:Genome, Viral; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017434:Protein Structure, Tertiary / Q000235:genetics; D020365:RNA Helicases / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D012324:RNA-Dependent RNA Polymerase; D003546:Cysteine Endopeptidases; D020365:RNA Helicases