title: Simultaneous determination of ginsenoside Rb(1) and Rg(1) in human plasma by liquid chromatography-mass spectrometry.
abstract: A liquid chromatographic-mass spectrometric (LC/MS) method for the simultaneous determination of ginsenoside Rb(1) and Rg(1) in human plasma was developed. The method involved the protein precipitation followed by analysis of ginsenoside Rb(1) and Rg(1) in an Atlantis C(18) column with the gradient elution of acetonitrile and ammonium formate (10mM, pH 3.0) at a flow rate of 0.2 ml/min. The analytes were determined using electrospray negative ionization mass spectrometry in the selected ion monitoring mode. The standard curves for ginsenoside Rb(1) and Rg(1) were linear over the concentration range of 10.0-1000 ng/ml. The lower limit of quantification was 10.0 ng/ml using 100 microl plasma sample. The coefficient of variation of intra- and inter-day assays for ginsenoside Rb(1) and Rg(1) at three quality control levels ranged from 1.0 to 6.8% and 5.4 to 9.8%, respectively. Ginsenoside Rb(1) and Rg(1) were stable in blank human plasma at room temperature for 24h and following three freeze-thaw cycles.
authors.affiliation: College of Pharmacy, Wonkwang University, Shinyongdong 344-2, Jeonbuk, Iksan 570749, South Korea.
authors.forename: Hye Young
authors.initials: HY
authors.lastname: Ji
authors.forename: Hye Won
authors.initials: HW
authors.lastname: Lee
authors.forename: Hae Kyoung
authors.initials: HK
authors.lastname: Kim
authors.forename: Hui Hyun
authors.initials: HH
authors.lastname: Kim
authors.forename: Seung Goo
authors.initials: SG
authors.lastname: Chang
authors.forename: Dong Hwan
authors.initials: DH
authors.lastname: Sohn
authors.forename: Jaebaek
authors.initials: J
authors.lastname: Kim
authors.forename: Hye Suk
authors.initials: HS
authors.lastname: Lee
mesh_terms: D002138:Calibration; D002853:Chromatography, Liquid; D036145:Ginsenosides / Q000097:blood*; D006801:Humans; D008297:Male; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D036145:Ginsenosides; C048026:ginsenoside Rb2; C442759:ginsenoside Rb1