Identification of 20 microRNAs from Oryza sativa.
MicroRNAs (miRNAs) are present in both plant and animal kingdoms and represents a growing family of non-coding RNAs. These tiny RNAs act as small guides and direct negative regulations usually in the process of development through sequence complementarity to target mRNAs. Although a large number of miRNAs have been identified from various animals, so far plant miRNA studies have focused mainly on Arabidopsis. Here we describe the identification of 20 miRNAs from a rice cDNA library. All the miRNAs were presumably processed from precursors with stem-loop structures and were positively detected in rice cells from at least one tissue, some of which showed tissue-specific expression. Twenty-three unique rice genes were identified to be feasible targets for seven rice miRNAs, including four members of Scarecrow-like transcription factor, the targets of miR-39 that had been characterized in Arabidopsis. Lacking long complementarity, the regulatory targets of 13 miRNAs remain to be further investigated. A possible mechanism of translational repressor for plant miRNAs that lack perfect complementarity to target mRNAs is discussed.
Wang
Jia-Fu
JF
Key Laboratory of Gene Engineering of the Ministry of Education, Biotechnology Research Center, Zhongshan University, Guangzhou 510275, People's Republic of China.
Zhou
Hui
H
Chen
Yue-Qin
YQ
Luo
Qing-Jun
QJ
Qu
Liang-Hu
LH
D001483:Base Sequence; D015723:Gene Library; D018745:Genome, Plant; D035683:MicroRNAs / Q000032:analysis* / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D018749:RNA, Plant / Q000032:analysis* / Q000378:metabolism
D035683:MicroRNAs; D018749:RNA, Plant