Layer-by-layer deposition of hyaluronic acid and poly-L-lysine for patterned cell co-cultures.
A novel method for patterning cellular co-cultures that uses the layer-by-layer deposition of ionic biopolymers is described. Non-biofouling hyaluronic acid (HA) micropatterns were used to immobilize cells and proteins to glass substrates. Subsequent ionic adsorption of poly-L-lysine (PLL) to HA patterns was used to switch the HA surfaces from cell repulsive to adherent thereby facilitating the adhesion of a second cell type. The utility of this approach to pattern co-cultures of hepatocytes or embryonic stem cells with fibroblasts was demonstrated. In addition, the versatility of this approach to generate patterned co-cultures irrespective of the primary cell seeding and relative adhesion of the seeded cells was demonstrated. Thus, the proposed method may be a useful tool for fabricating controlled cellular co-cultures for cell-cell interaction studies and tissue engineering applications.
Khademhosseini
Ali
A
Division of Biological Engineering, Massachusetts Institute of Technology, 45 Carleton Street, E25-342, Cambridge, MA 02139, USA.
Suh
Kahp Y
KY
Yang
Jen M
JM
Eng
George
G
Yeh
Judy
J
Levenberg
Shulamit
S
Langer
Robert
R
D000327:Adsorption; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D018914:Cells, Immobilized / Q000187:drug effects / Q000502:physiology; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D018920:Coculture Techniques / Q000379:methods*; D022781:Hepatocytes / Q000166:cytology* / Q000187:drug effects*; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology*; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D011107:Polylysine / Q000737:chemistry* / Q000494:pharmacology*; D011485:Protein Binding; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods
D020099:Coated Materials, Biocompatible; D011107:Polylysine; D006820:Hyaluronic Acid