title: [Diagnosis of Mycobacterium ulcerans infection by PCR: report of 3 cases observed in French Guiana].
abstract: Mycobacterium ulcerans infection is the third most important mycobacterial infection in the world. It has been described in many different countries including French Guiana. The diagnosis of M. ulcerans infection by culture is often difficult because culture is hard to perform in endemic areas and their sensitivity is not reliable. As a result the diagnosis of this infection is often delayed. However, molecular methods are now available to diagnose rapidly infections by M. ulcerans and distinguish it from other mycobacteria. We report three cases of skin infection due to M. ulcerans observed in French Guiana. Diagnosis was initially made by polymerase chain reaction and was confirmed later by culture (in two patients) and inoculation to mice (in one patient). A faster diagnosis of M. ulcerans infection should lead to a better prognosis of this infection.
authors.affiliation: Service des maladies infectieuses et tropicales, CHU Nord, chemin des Bourrelys, 13015 Marseille, France. ameliemenard@hotmail.com
authors.forename: A
authors.initials: A
authors.lastname: Ménard
authors.forename: P
authors.initials: P
authors.lastname: Couppié
authors.forename: D
authors.initials: D
authors.lastname: Sainte-Marie
authors.forename: R
authors.initials: R
authors.lastname: Pradinaud
mesh_terms: D000328:Adult; D005260:Female; D005616:French Guiana; D006801:Humans; D008297:Male; D008875:Middle Aged; D009165:Mycobacterium Infections, Nontuberculous / Q000175:diagnosis*; D019911:Mycobacterium ulcerans; D016133:Polymerase Chain Reaction