First record of Cryptosporidium infection in a raccoon dog (Nyctereutes procyonoides viverrinus).
Cryptosporidium species have been found in more than 150 species of mammals, but there has been no report in raccoon dogs. Here we found the Cryptosporidium organism in a raccoon dog, Nyctereutes procyonoides viverrinus, and identified this isolate using PCR-based diagnostic methods. Cryptosporidium diagnostic fragments of the 18S ribosomal RNA, Cryptosporidium oocyst wall protein and 70-kDa heat shock protein genes were amplified from the isolate and sequenced to reveal the phylogenetic relationships between it and other Cryptosporidium species or genotypes reported previously. The results showed that the raccoon dog isolate represented the C. parvum cattle genotype which could be a causative agent in human cryptosporidiosis.
Matsubayashi
Makoto
M
Department of Food and Nutrition, Osaka Joshi-Gakuen Junior College, Tennoji-ku, Osaka 543-0073, Japan. matsu@ojg.ac.jp
Abe
Niichiro
N
Takami
Kazutoshi
K
Kimata
Isao
I
Iseki
Motohiro
M
Nakanishi
Teruo
T
Tani
Hiroyuki
H
Sasai
Kazumi
K
Baba
Eiichiroh
E
D000818:Animals; D002335:Carnivora / Q000469:parasitology*; D003457:Cryptosporidiosis / Q000469:parasitology / Q000662:veterinary*; D016785:Cryptosporidium parvum / Q000235:genetics / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA
D016054:DNA, Protozoan; D018840:HSP70 Heat-Shock Proteins; D015800:Protozoan Proteins; D012337:RNA, Ribosomal, 18S