title: In vitro comparative kinetics of adhesive and haemolytic potential of T. vaginalis isolates from symptomatic and asymptomatic females.
abstract: The host parasite relationship and pathogenic mechanisms of the commonly reported sexually transmitted urogenital disease, trichomoniasis, are poorly understood. This study was planned to correlate the adhesion properties of Trichomonas vaginalis isolates from symptomatic and asymptomatic women to vaginal epithelial cells in vitro (in presence and absence of L. acidophilus) and to ascertain the haemolytic activity of the isolates, in order to assess these properties as possible markers of pathogenicity. Cytoadherence assay study shows the significant difference in adhesion only up to first 15 minutes of incubation in symptomatic versus asymptomatic isolates. The presence of L. acidophilus was found to be more effective in enhancing the attachment of T. vaginalis in a time dependent manner mostly operative through its pH lowering effect, whereas the excretory secretory products of L. acidophilus reduced the attachment in case of both symptomatic and asymptomatic isolates. Amount of haemoglobin released by isolates from symptomatic patients was significantly higher than by the isolates from asymptomatic women. This investigation forms the basis for future studies to explore the role of other known virulence factors of T. vaginalis in initiation and persistence of vaginal infection by the parasite.
authors.affiliation: Department of Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh.
authors.forename: Zarrintaj
authors.initials: Z
authors.lastname: Valadkhan
authors.forename: Saroj
authors.initials: S
authors.lastname: Sharma
authors.forename: Kusum
authors.initials: K
authors.lastname: Harjai
authors.forename: Indu
authors.initials: I
authors.lastname: Gupta
authors.forename: Nancy
authors.initials: N
authors.lastname: Malla
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D004847:Epithelial Cells / Q000469:parasitology; D005260:Female; D006461:Hemolysis; D006790:Host-Parasite Interactions; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D007779:Lactobacillus acidophilus / Q000502:physiology; D014247:Trichomonas Vaginitis / Q000469:parasitology*; D014246:Trichomonas vaginalis / Q000302:isolation & purification / Q000472:pathogenicity*; D014621:Vagina / Q000469:parasitology; D014774:Virulence