Identification of the feline CD63 homologue using retrovirus-mediated expression cloning.
Recently, we combined a retrovirus-mediated expression cloning with a simple screening method using non-adherent cells and panning [Anal. Biochem. 315 (2003) 138]. In this study, we applied this method to identify the antigen recognized by an uncharacterized monoclonal antibody raised against a feline cell line, and identified it as the feline homologue of CD63. This simple method is useful for characterizing unknown antibodies that recognize cell surface molecules. Furthermore, the monoclonal antibody identified as an anti-feline CD63 antibody will be useful for studying feline molecular function(s).
Sakurai
Yumiko
Y
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Shimojima
Masayuki
M
Miyazawa
Takayuki
T
Masuoka
Kouta
K
Tohya
Yukinobu
Y
Akashi
Hiroomi
H
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000235:genetics* / Q000276:immunology*; D001483:Base Sequence; D002415:Cats / Q000235:genetics* / Q000276:immunology*; D045744:Cell Line, Tumor; D003001:Cloning, Molecular / Q000379:methods; D005434:Flow Cytometry / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D015723:Gene Library; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000235:genetics; D010980:Platelet Membrane Glycoproteins / Q000235:genetics* / Q000276:immunology*; D016415:Sequence Alignment; D060149:Tetraspanin 30
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30