Immunomodulatory and antitumor effects in vivo by the cytoplasmic fraction of Lactobacillus casei and Bifidobacterium longum.
The immunomodulatory and antitumor effects of lactic acid bacteria (LABs) were investigated. Cytoplasmic fraction of Lactobacillus acidophilus, Lactobacillus casei and Bifidobacterium longum were tested for the antiproliferative activity in vitro to SNUC2A, SNU1, NIH/3T3 and Jurkat cell lines by crystal violet assay. All cytoplasmic fraction suppressed proliferation of tumor cells, though L. casei and B. longum were more effective. From these results, cytoplasmic fraction of L. casei and B. longum with Y400 as a control were administered as dietary supplements to Balb/c mice for 2, and 4 consecutive wks. Administration for 4 wks enhanced the number of total T cells, NK cells and MHC class II+ cells, and CD4-CD8+ T cells in flow cytometry analysis. To determine of antitumor activity of LABs preparation in vivo, F9 teratocarcinoma cells were inoculated on mice at 14th day. Body weight was decreased with increased survival rate in all groups with the cytoplasm of LABs. Our results showed that cytoplasmic fraction of LABs had direct antiproliferative effects on tumor cell lines in vitro, effects on immune cells in vivo, and antitumor effects on tumor-bearing mice with prolonged survival periods.
Lee
Jung-Woo
JW
Laboratory of Immunology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
Shin
Jung-Gul
JG
Kim
Eun Hee
EH
Kang
Hae Eun
HE
Yim
In Been
IB
Kim
Ji Yeon
JY
Joo
Hong-Gu
HG
Woo
Hee Jong
HJ
D016475:3T3 Cells; D000818:Animals; D001644:Bifidobacterium; D001835:Body Weight; D002455:Cell Division / Q000502:physiology; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D019169:Jurkat Cells; D007694:Killer Cells, Natural / Q000276:immunology; D007780:Lacticaseibacillus casei; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000276:immunology / Q000628:therapy*; D019936:Probiotics / Q000494:pharmacology*; D016019:Survival Analysis; D013601:T-Lymphocytes / Q000276:immunology