Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins.
Using Nalpha,Nalpha-bis(carboxymethyl)lysine to react with FePt magnetic nanoparticles, we synthesized the FePt-NTA conjugate, which immobilizes Ni2+ ions and selectively binds to histidine-tagged proteins at concentration as low as 0.5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins.
Xu
Chenjie
C
Department of Chemistry, Department of Physics, and Bioengineering Program, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China. chbingxu@ust.hk
Xu
Keming
K
Gu
Hongwei
H
Zhong
Xiaofen
X
Guo
Zhihong
Z
Zheng
Rongkun
R
Zhang
Xixiang
X
Xu
Bing
B
D000497:Alloys / Q000737:chemistry; D006639:Histidine / Q000737:chemistry*; D007501:Iron / Q000737:chemistry; D008280:Magnetics; D036103:Nanotechnology / Q000379:methods*; D009571:Nitrilotriacetic Acid / Q000737:chemistry*; D010316:Particle Size; D010984:Platinum / Q000737:chemistry; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
D000497:Alloys; D011506:Proteins; D010984:Platinum; D006639:Histidine; D007501:Iron; D009571:Nitrilotriacetic Acid