Development of novel conductometric biosensors based on immobilised whole cell Chlorella vulgaris microalgae.
A novel biosensor based on immobilised whole cell Chlorella vulgaris microalgae as a bioreceptor and interdigitated conductometric electrodes as a transducer has been developed and tested for alkaline phosphatase activity (APA) analysis. These sensors were also used for the detection of toxic compounds, namely cadmium ions, in aquatic habitats. Algae were immobilised inside bovine serum albumin (BSA) membranes cross-linked with glutaraldehyde vapours. The detection of the local conductivity variations caused by algae enzymatic reactions could be achieved. The inhibition of C. vulgaris microalgae Alkaline phosphatase activities in presence of cadmium ions was measured. These results were compared with measurements in bioassays. It finally appeared that conductometric biosensors using algae seemed more sensitive than bioassays to detect low levels of cadmium ions (the detection limit for the first experiments was 1 ppb of Cd2+). The main advantages of these alkaline phosphatase biosensors consist of their high specificity in regard to the toxic compounds they enable to detect, but also on their high stability since contrary to enzymatic biosensors, they use whole algae cells with APs on their walls.
Chouteau
Celine
C
Laboratoire des Sciences de l'Evnironnement, Ecole Nationale des Travaux Publics de l'Etat, rue Maurice Audin, 69518 Vaulx-en-Velin Cedex, France.
Dzyadevych
Sergei
S
Chovelon
Jean-Marc
JM
Durrieu
Claude
C
D000469:Alkaline Phosphatase / Q000032:analysis* / Q000378:metabolism; D001681:Biological Assay; D015374:Biosensing Techniques; D002104:Cadmium; D002708:Chlorella; D004553:Electric Conductivity; D006923:Hymecromone / Q000031:analogs & derivatives / Q000378:metabolism; D009596:Nitrophenols / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D013997:Time Factors
D009596:Nitrophenols; D009943:Organophosphorus Compounds; D002104:Cadmium; C008644:nitrophenylphosphate; D006923:Hymecromone; D000469:Alkaline Phosphatase