[Design of chimeric proteins based on pentameric superhelical fragments of COMP. I. Properties of a hybrid containing an immunodominant segment of the surface protein of Plasmodium falciparum].
The oligomeric recombinant protein DB-1 containing the immunodominant sites of the circumsporozoite protein of Plasmodium falciparum and tetanus toxin was constructed to optimize the schemes of presentation of B-cell epitopes during vaccination with chimeric proteins without the use of adjuvants. A fragment of the pentameric coiled-coil human cartilage oligomeric matrix protein was used as an oligomerization matrix. The expression of the protein in Escherichia coli cells was studied, a method for its purification was developed, and it was biochemically characterized. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 1; see also http://www.maik.ru.
Belitskaia
S Iu
SIu
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP Moscow, 117997 Russia. lap08@mail.ru
Boldyreva
E F
EF
Korobko
V G
VG
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001483:Base Sequence; D001704:Biopolymers; D064236:Cartilage Oligomeric Matrix Protein; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins / Q000737:chemistry* / Q000235:genetics; D006023:Glycoproteins / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D016056:Immunodominant Epitopes / Q000737:chemistry* / Q000276:immunology; D064235:Matrilin Proteins; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000737:chemistry*; D011487:Protein Conformation; D015800:Protozoan Proteins / Q000737:chemistry* / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics
D001704:Biopolymers; D064236:Cartilage Oligomeric Matrix Protein; D017931:DNA Primers; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D016056:Immunodominant Epitopes; D064235:Matrilin Proteins; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; C577553:TSP5 protein, human; C041595:circumsporozoite protein, Protozoan