Staurosporine induces apoptotic volume decrease (AVD) in ECV304 cells.
Incubation of ECV304 cells with 1 micro M staurosporine (STS) causes apoptotic cell death. In the present study, we investigate whether a significant apoptotic volume decrease (AVD) was apparent during the very early times (1 h) of the apoptotic process. Our data suggest that upregulation of Cl(-) (and possibly K(+)) channels by STS may be a very early primary event required for the subsequent onset of AVD, which results in apoptosis.
Porcelli
A M
AM
Dipartimento di Biologia Ev. Sp., Dipartimento di Fisiologia Umana e Generale, Universita di Bologna, 40126 Bologna, Italy. porcelli@alma.unibo.it
Ghelli
A
A
Zanna
C
C
Valente
P
P
Ferroni
S
S
Rugolo
M
M
D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D048429:Cell Size / Q000187:drug effects*; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins / Q000032:analysis; D011994:Recombinant Proteins / Q000032:analysis; D019311:Staurosporine / Q000494:pharmacology*; D014162:Transfection
D008164:Luminescent Proteins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D019311:Staurosporine