title: Speciation of V, Cr and Fe by capillary electrophoresis-bandpass reaction cell inductively coupled plasma mass spectrometry.
abstract: Capillary electrophoresis-dynamic reaction cell inductively coupled plasma mass spectrometry (CE-DRC-ICP-MS) for the speciation of iron(III/II), vanadium(V/IV) and chromium(VI/III) is described. Two different CE migration modes were employed for separating the six metal ions using pre-capillary complexation. One is counter-electroosmotic mode in which iron(III/II) and vanadium(V/IV) ions were well separated using a 60 cm x 75 microm i.d. fused silica capillary. The voltage was set at +22 kV and a 15 mmol l(-1) tris(hydroxymethyl)aminomethane (Tris) buffer (pH 8.75) containing 0.5 mmol l(-1) ethylenediaminetetraacetic acid (EDTA) and 0.5 mmol l(-1) ortho-phenanthroline (phen) was used as the electrophoretic buffer. The other is co-electroosmotic mode in which chromium(VI/III) ions were well separated while the applied voltage was set at -22 kV and a 10 mmol l(-1) ammonium citrate buffer (pH 7.7) containing 0.5 mmol l(-1) diethylenetriaminepentaacetic acid (DTPA) and 0.01% polybrene was used as the electrophoretic buffer. The mass spectra were measured at m/z 51, 52 and 56 for V. Cr and Fe, respectively. The interfering polyatomic ions of 35Cl16O+, 40Ar12C+ and 40Ar16O+ on 51V+, 52Cr+ and 56Fe+ determination were reduced in intensity significantly by using NH3 as the reaction cell gas in the DRC. The detection limits were in the range of 0.1-0.5, 0.4-1.3 and 1.2-1.7 ng ml(-1) for V, Cr and Fe, respectively. Applications of the method for the speciation of V, Cr and Fe in wastewater were demonstrated. The recoveries were in the range of 92-120% for various species.
authors.affiliation: Department of Chemistry, National Sun Yat-sen University, 70 Lien-hai Road, Kaohsiung 80424, Taiwan.
authors.forename: Ching-Fen
authors.initials: CF
authors.lastname: Yeh
authors.forename: Shiuh-Jen
authors.initials: SJ
authors.lastname: Jiang
mesh_terms: D002857:Chromium / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007501:Iron / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D014639:Vanadium / Q000032:analysis*
chemical_list: D014639:Vanadium; D002857:Chromium; D007501:Iron