Characterization of lymphocyte subpopulations and major histocompatibility complex haplotypes of mastitis-resistant and susceptible cows.
Bovine mastitis is an infectious disease with a major economic influence on the dairy industry worldwide. Many factors such as environment, pathogen, and host affect susceptibility or resistance of an individual cow to bovine mastitis. Recently, there has been considerable interest in defining genetic and immunological markers that could be used to select for improved disease resistance. In this study we have analyzed the lymphocyte subpopulations of mastitis-resistant and susceptible cows using monoclonal antibodies specific for bovine leukocyte differentiation antigens and flow cytometry. We have also used a microarray typing technique to define the bovine leukocyte antigen (BoLA) class I and class II haplotypes associated with resistance or susceptibility to bovine mastitis. A striking finding of the present study is that susceptibility to mastitis was associated with major histocompatibility complex (MHC) haplotypes that have only a single set of DQ genes. The study also revealed that susceptible cows had CD4:CD8 ratios of less than one in both their mammary gland secretions and peripheral blood. These results raise the possibility that the number of DQ genes that a cow has and/or a cow's CD4:CD8 ratio could be used as indicators of susceptibility to bovine mastitis.
Park
Yong Ho
YH
Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.
Joo
Yi Seok
YS
Park
Joo Youn
JY
Moon
Jin San
JS
Kim
So Hyun
SH
Kwon
Nam Hoon
NH
Ahn
Jong Sam
JS
Davis
William C
WC
Davies
Christopher J
CJ
D000483:Alleles; D000818:Animals; D000943:Antigens, Differentiation / Q000276:immunology; D002417:Cattle; D002452:Cell Count / Q000662:veterinary; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D020022:Genetic Predisposition to Disease; D006649:Histocompatibility Antigens / Q000235:genetics / Q000276:immunology; D007723:Korea; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D016131:Lymphocyte Subsets / Q000276:immunology* / Q000382:microbiology; D008414:Mastitis, Bovine / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary; D018709:Statistics, Nonparametric
D000943:Antigens, Differentiation; D006649:Histocompatibility Antigens