Analysis of competitive binding of ligands to human serum albumin using NMR relaxation measurements.
The competitive binding of two ligands, ibuprofen (IBP) and salicylic acid (SAL), to human serum albumin (HSA) was studied by using nuclear magnetic resonance (NMR) relaxation measurements. When the concentration of one ligand was increased in the solution containing IBP, SAL and HSA, the fractions of free IBP and SAL were increased because of the competitive binding. The 1H relaxation rates (R1) of both ligands were subsequently decreased. If a ligand is in fast exchanging between the free and bound forms, the observed 1H relaxation rate is a weighted average of that for the free ligand and the protein-ligand complex. The concentrations of the free and bound ligands can be quantitatively derived from the relaxation rates. The results presented in this work revealed that IBP and SAL shared certain low-affinity binding sites on the HSA molecule, in addition to the same high-affinity binding site of AIII.
Cui
Y F
YF
State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, PR China.
Bai
G Y
GY
Li
C G
CG
Ye
C H
CH
Liu
M L
ML
D006801:Humans; D007052:Ibuprofen / Q000378:metabolism*; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011485:Protein Binding / Q000502:physiology; D020156:Salicylic Acid / Q000378:metabolism*; D012709:Serum Albumin / Q000378:metabolism*
D008024:Ligands; D012709:Serum Albumin; D020156:Salicylic Acid; D007052:Ibuprofen