[Isolation and properties of glucose oxidase from Penicillum funiculosum 433].
A method for isolation of extracellular glucose oxidase from Penicillium funiculosum 433 and its purification is proposed. The enzymatic preparation was produced with a yield of 56% and a specific activity of 3730 AU per 1 mg protein. The enzyme studied displayed a high thermostability, resistance to metal ions, and performance in a wide pH range and was equal in its properties to foreign analogues.
Sukhacheva
M V
MV
Lomonosov Moscow State University, Moscow, 119899 Russia. msukhacheva@mail.ru
Davydova
M E
ME
Netrusov
A I
AI
D002853:Chromatography, Liquid / Q000379:methods; D004795:Enzyme Stability; D005949:Glucose Oxidase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010407:Penicillium / Q000201:enzymology*; D013696:Temperature
D005949:Glucose Oxidase