title: Association of stomatin with lipid bodies.
abstract: The oligomeric lipid raft-associated integral protein stomatin normally localizes to the plasma membrane and the late endosomal compartment. Similar to the caveolins, it is targeted to lipid bodies (LBs) on overexpression. Endogenous stomatin also associates with LBs to a small extent. Green fluorescent protein-tagged stomatin (StomGFP) and the dominant-negative caveolin-3 mutant DGV(cav3)HA occupy distinct domains on LB surfaces but eventually intermix. Studies of StomGFP deletion mutants reveal that the region for membrane association but not oligomerization and raft association is essential for LB targeting. Blocking protein synthesis leads to the redistribution of StomGFP from LBs to LysoTracker-positive vesicles indicating a connection with the late endosomal/lysosomal pathway. Live microscopy of StomGFP reveals multiple interactions between LBs and microtubule-associated vesicles possibly representing signaling events and/or the exchange of cargo. Proteomic analysis of isolated LBs identifies adipophilin and TIP47, various lipid-specific enzymes, cytoskeletal components, chaperones, Ras-related proteins, protein kinase D2, and other regulatory proteins. The association of the Rab proteins 1, 6, 7, 10, and 18 with LBs indicates various connections to other compartments. Our data suggest that LBs are not only involved in the storage of lipids but also participate actively in the cellular signaling network and the homeostasis of lipids.
authors.affiliation: Institute of Biochemistry, Medical University of Vienna, Vienna Biocenter, Vienna A-1030, Austria.
authors.forename: Ellen
authors.initials: E
authors.lastname: Umlauf
authors.forename: Edina
authors.initials: E
authors.lastname: Csaszar
authors.forename: Manuel
authors.initials: M
authors.lastname: Moertelmaier
authors.forename: Gerhard J
authors.initials: GJ
authors.lastname: Schuetz
authors.forename: Robert G
authors.initials: RG
authors.lastname: Parton
authors.forename: Rainer
authors.initials: R
authors.lastname: Prohaska
mesh_terms: D000818:Animals; D001692:Biological Transport; D001798:Blood Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003594:Cytoplasmic Granules / Q000378:metabolism*; D004285:Dogs; D006801:Humans; D050356:Lipid Metabolism; D021962:Membrane Microdomains / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D001798:Blood Proteins; D008565:Membrane Proteins; C036860:STOM protein, human