title: Mitochondrial membrane potential after low-power laser irradiation.
abstract: We used the lipophilic cationic fluorescent dye 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimidazol-carbocyanine iodide (JC-1) to determine mitochondrial membrane potential (mdeltapsi) in Hep-2 cells after irradiation with low-power laser (lambda=635 nm). Through this methodology it was possible to analyze the variation on mitochondrial number and mdeltapsi, in cells irradiated for 100, 150 and 200 s with energy density of 100 mJ/cm(2). Our results show that JC-1 dye allows the identification of populations with different mitochondria morphology as well as the functionality of this organelle in the cells incubated for 1, 6 and 24 h, after irradiation with low-power laser.
authors.affiliation: Laboratório de Biologia Celular e Tecidual, Instituto de Pesquisa e Desenvolvimento, Universidade do Vale do Paraíba, Av. Shishima Hifumi 2911, 12244-000, São José dos Campos, S P, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Bortoletto
authors.forename: N S
authors.initials: NS
authors.lastname: Silva
authors.forename: R A
authors.initials: RA
authors.lastname: Zângaro
authors.forename: M T T
authors.initials: MT
authors.lastname: Pacheco
authors.forename: R A
authors.initials: RA
authors.lastname: Da Matta
authors.forename: C
authors.initials: C
authors.lastname: Pacheco-Soares
mesh_terms: D001562:Benzimidazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D006801:Humans; D028022:Low-Level Light Therapy / Q000379:methods*; D008564:Membrane Potentials; D008566:Membranes / Q000502:physiology; D008928:Mitochondria / Q000502:physiology*
chemical_list: D001562:Benzimidazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; C068624:5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine