title: Specific gene silencing in the pre-implantation stage mouse embryo by an siRNA expression vector system.
abstract: Recently, small interfering RNAs (siRNAs) have become a powerful and widely used tool for the analysis of gene function in mammalian cells. Here we report that the microinjection of an siRNA expression vector into the nucleus is an efficient and powerful method of specific gene silencing in pre-implantation mouse embryos. We used this method to examine the expression of two genes EGFP and Oct4. Vectors encoding siRNAs targeted against EGFP or Oct4 were injected into the pronucleus or nucleus of zygotes, which were then cultured until the blastocyst stage. When the effects of RNAi were examined in blastocyst stage eggs, there was robust inhibition of the gene product in a concentration-dependent manner at both the mRNA and the protein level. The expression of other endogenous genes was not affected, showing the specificity of the vector-mediated RNAi. In addition, this method was effective for inhibiting maternally expressed mRNA. To demonstrate that RNAi of Oct4 induced a similar phenotype to that of Oct4-null embryos, the blastocysts were further cultured in ES medium. After the fourth day of culture, the embryos either had outgrown only a layer of trophoblast cells or showed developmental arrest at the blastocyst stage (>90%). Moreover, concomitant with Oct4 suppression at the blastocyst stage, we observed inhibition of Fgf4, a gene that is known to be induced downstream of Oct4 expression. Taken together, these results demonstrate that the use of siRNA expression vector is a powerful way to achieve gene silencing in the pre-implantation stage embryo.
authors.affiliation: Department of Microbiology, Shiga University of Medical Science, Seta, Otsu, Shiga, Japan. sh431@mole.bio.cam.ac.uk
authors.forename: Seiki
authors.initials: S
authors.lastname: Haraguchi
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Saga
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Naito
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Inoue
authors.forename: Akira
authors.initials: A
authors.lastname: Seto
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000235:genetics; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D047108:Embryonic Development / Q000235:genetics*; D005111:Extrachromosomal Inheritance / Q000235:genetics; D005260:Female; D020868:Gene Silencing; D005822:Genetic Vectors / Q000235:genetics*; D008297:Male; D051379:Mice; D050814:Octamer Transcription Factor-3; D010641:Phenotype; D011247:Pregnancy; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000172:deficiency / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D034741:RNA, Small Interfering; D014157:Transcription Factors