Recent developments for SPIE-IA, a new sandwich immunoassay format for very small molecules.
Recent publications describing new elegant approaches to assay small analytes using noncompetitive format were briefly reviewed. Among these methods, we have developed a new protocol, named SPIE-IA, which involves a cross-linking step achieved using chemical hombifunctional reagents, UV irradiation or free radicals. This new method proved to be useful to detect naturally occurring analyte/antibody complexes or to protect the analytes against degradation by peptidases. On the other hand, SPIE-IA could allow to study the adverse biological effects of UV and some aspects of free radical chemistry or to evaluate the antioxidant activity of molecules.
Volland
Hervé
H
CEA, Service de Pharmacologie et d'Immunologie, DRM/DSV, CEA/Saclay, 91191 Gif-sur-Yvette Cedex, France. herve.volland@cea.fr
Pradelles
Philippe
P
Taran
Frédéric
F
Buscarlet
Laure
L
Creminon
Christophe
C
D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000032:analysis; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D005602:France; D005609:Free Radicals / Q000032:analysis; D007118:Immunoassay / Q000379:methods* / Q000639:trends*; D018448:Models, Immunological; D014466:Ultraviolet Rays / Q000009:adverse effects
D000975:Antioxidants; D003432:Cross-Linking Reagents; D000939:Epitopes; D005609:Free Radicals