title: One-hybrid screens at the Saccharomyces cerevisiae HMR locus identify novel transcriptional silencing factors.
abstract: In Saccharomyces cerevisiae, genes located at the telomeres and the HM loci are subject to transcriptional silencing. Here, we report results of screening a Gal4 DNA-binding domain hybrid library for proteins that cause silencing when targeted to a silencer-defective HMR locus.
authors.affiliation: Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York 11794, USA.
authors.forename: Erik D
authors.initials: ED
authors.lastname: Andrulis
authors.forename: David C
authors.initials: DC
authors.lastname: Zappulla
authors.forename: Krassimira
authors.initials: K
authors.lastname: Alexieva-Botcheva
authors.forename: Carlos
authors.initials: C
authors.lastname: Evangelista
authors.forename: Rolf
authors.initials: R
authors.lastname: Sternglanz
mesh_terms: D004268:DNA-Binding Proteins; D020868:Gene Silencing; D005800:Genes, Fungal; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae / Q000235:genetics; D016615:Telomere / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D020798:Two-Hybrid System Techniques
chemical_list: D004268:DNA-Binding Proteins; C052130:GAL4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae; D014157:Transcription Factors