Loss of heterozygosity on 10q and mutational status of PTEN and BMPR1A in colorectal primary tumours and metastases.
We investigated the possible role of chromosome 10q losses in colorectal cancer metastasis by carrying out an allelic imbalance study on a series of microsatellite instability-negative (MSI-) primary tumours (n=32) and metastases (n=36) from 49 patients. Our results demonstrate that 10q allelic losses are associated with a significant proportion (25%) of MSI- colorectal tumours, but are not involved in the metastatic process. PTEN and BMPR1A, two genes located in the common deleted region, were screened for mutations in samples with loss of heterozygosity. The absence or low frequency of mutations indicates that the inactivation of these genes by deletion of one allele and mutation of the other one plays only a minor role in MSI- tumours.
Karoui
M
M
Fédération des Spécialités Digestives, Hôpital Ambroise Paré, AP-HP, Université Versailles-Saint Quentin en Yvelines, 92104 Boulogne Cedex, France.
Tresallet
C
C
Julie
C
C
Zimmermann
U
U
Staroz
F
F
Brams
A
A
Muti
C
C
Boulard
C
C
Robreau
A-M
AM
Puy
H
H
Malafosse
R
R
Penna
C
C
Pruvot
F-R
FR
Thiery
J P
JP
Boileau
C
C
Rougier
P
P
Nordlinger
B
B
Radvanyi
F
F
Franc
B
B
Hofmann-Radvanyi
H
H
D001483:Base Sequence; D052005:Bone Morphogenetic Protein Receptors, Type I; D002879:Chromosomes, Human, Pair 10; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D016147:Genes, Tumor Suppressor; D018095:Germ-Line Mutation; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D051059:PTEN Phosphohydrolase; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D017978:Receptors, Growth Factor / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000235:genetics*
D017978:Receptors, Growth Factor; D025521:Tumor Suppressor Proteins; D017346:Protein Serine-Threonine Kinases; C498211:BMPR1A protein, human; D052005:Bone Morphogenetic Protein Receptors, Type I; D010744:Phosphoric Monoester Hydrolases; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human