Early activation and induction of apoptosis in T cells is independent of c-Fos.
We used c-Fos-deficient activated T cells from the spleen and c-Fos-deficient thymocytes to address the capacity of these cells to undergo apoptosis in response to various stimuli. To determine the role of c-Fos in apoptosis regulation in thymocytes, we challenged thymocytes from wild-type and c-Fos-deficient mice with either TPA or the glucocorticoid dexamethasone. After various time points cells were stained according to the Nicoletti method and analyzed by FACS. Thymocytes from both genotypes exhibited similar efficiency of apoptosis in response to treatment with TPA or dexamethasone. Our data provide clear evidence that c-Fos is not required for apoptosis regulation in activated T cells as well as in thymocytes.
Bierbaum
Hanna
H
Division of Signal Transduction and Growth Control, Deutsches Krebsforschungszentrum Heidelberg, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany.
Baumann
Sven
S
Herr
Ingrid
I
Tuckermann
Jan P
JP
Hess
Jochen
J
Schorpp-Kistner
Marina
M
Angel
Peter
P
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D016762:Genes, fos; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D016760:Proto-Oncogene Proteins c-fos / Q000502:physiology*; D013154:Spleen / Q000166:cytology / Q000502:physiology; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D013950:Thymus Gland / Q000166:cytology / Q000502:physiology
D016760:Proto-Oncogene Proteins c-fos; D003208:Concanavalin A