title: Electrochemical behavior and determination of amiloride drug in bulk form and pharmaceutical formulation at mercury electrodes.
abstract: The polarographic behavior of amiloride hydrochloride has been studied in Britton-Robinson buffers of pH 1.9-11. In acidic medium at Ph< or =2, the dc-polarograms exhibited a single 4-electron cathodic irreversible wave, while at pH values >2, a second two-electron irreversible cathodic wave appeared at a more negative potential. The single or first wave may be attributed to the cleavage of the double bond of the -CH=NH of the imidino amide group with the release of NH(3). While the second wave may be due to the saturation of the C=O of the carboxamide moiety. A polarographic procedure of suffocate sensitivity for the determination of bulk amiloride drug in Britton-Robinson buffer at pH 2 is described. The calibration graph was obtained over the concentration range 2.5 x 10(-5) to 2.5 x 10(-4) M amiloride. The limits of detection (LOD) and quantitation (LOQ) of the procedure were 1 x 10(-5) and 3.3 x 10(-4) M bulk amiloride, respectively. Moreover, a differential-pulse adsorptive cathodic stripping voltammetric procedure has been described to assay of the drug at lower concentration levels. The optimal conditions were: E(acc) = -0.9V, t(acc)=30 s, scan rate=20mV, pulse-height=90 mV and Britton-Robinson buffer of pH 8. The calibration graph was obtained over the concentration range 2 x 10(-8) to 1 x 10(-6) M for bulk amiloride. Both procedures were successfully applied to the determination of amiloride in tablets without the necessity for sample pretreatment or any time-consuming extraction or evaporation steps prior to the drug analysis.
authors.affiliation: Chemistry Department, Faculty of Science, Tanta University, 31527 Tanta, Egypt. gadbelhefnawy@hotmail.com
authors.forename: G B
authors.initials: GB
authors.lastname: El-Hefnawy
authors.forename: I S
authors.initials: IS
authors.lastname: El-Hallag
authors.forename: E M
authors.initials: EM
authors.lastname: Ghoneim
authors.forename: M M
authors.initials: MM
authors.lastname: Ghoneim
mesh_terms: D000584:Amiloride / Q000032:analysis* / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D004563:Electrochemistry; D004566:Electrodes; D008628:Mercury / Q000032:analysis*
chemical_list: D000584:Amiloride; D008628:Mercury