Evidence for multiple levels of regulation of Oenococcus oeni clpP-clpL locus expression in response to stress.
A locus containing the clpP and clpL genes in the lactic acid bacterium Oenococcus oeni was studied. Real-time reverse transcription-PCR analysis revealed different induction factors involved in expression of these genes during stress. According to the conditions, clpP and clpL genes could be transcripted as two distinct transcripts or cotranscripted. The clpP promoter depended on the CtsR regulator, but surprisingly the clpL promoter did not. The amount of the clpL transcript depended on mRNA stability. This clp ATPase gene is at least controlled at the posttranscriptional level.
Beltramo
Charlotte
C
Laboratoire de Microbiologie, UMR-UB-INRA 1232, 21 000 Dijon, France.
Grandvalet
Cosette
C
Pierre
Fabrice
F
Guzzo
Jean
J
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D049071:Endopeptidase Clp; D015964:Gene Expression Regulation, Bacterial; D006095:Gram-Positive Cocci / Q000235:genetics* / Q000254:growth & development; D018869:Heat-Shock Response; D007934:Leuconostoc / Q000235:genetics / Q000254:growth & development; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D012697:Serine Endopeptidases; D049071:Endopeptidase Clp; D000251:Adenosine Triphosphatases