title: The effect of different extenders on post-thaw sperm survival, acrosomal integrity and longevity in cryopreserved semen of Formosan Sika deer and Formosan Sambar deer.
abstract: This study investigates the efficacy of five extenders in contributing to the outcome of semen cryopreservation in Formosan Sika and Sambar deer. Pooled semen (n=4) of six males of each breed was used. In Sika deer, semen collection rate was 96% (23/24) over all electro-ejaculations. Volume, sperm motility and sperm concentration of fresh ejaculates was 0.5+/-0.4 ml, 77+/-6% and 1471.3+/-940.0 x 10(6) ml(-1), respectively. Post-thaw motility in respective extender was A: 66+/-16%; B: 71+/-2%; C: 73+/-6%; D: 9+/-4% and E: 26+/-12% (mean+/-S.D.). In extender C (74+/-14%) more viable spermatozoa were preserved than in the others (A: 64+/-10%; B: 48+/-11%; D: 41+/-16%; E: 47+/-6%; P<0.05). Acrosomal integrity was not influenced by extender composition. Post-thaw motility did not decrease during a 4-h incubation period, irrespective of the extender used (P>0.05). In Sambar deer, semen collection rate was 88% (21/24) over all electro-ejaculations. Volume, sperm motility and sperm concentration of fresh ejaculates was 1.3+/-0.5 ml, 82+/-4% and 379.1+/-252.2 x 10(6) ml(-1), respectively. Post-thaw motility was in respective extenders A: 69+/-2%; B: 74+/-6%; C: 73+/-2%; D: 13+/-6% and E: 31+/-20%. Extenders B and C were superior (P>0.05) with respect to sperm motility. Similarly, post-thaw viability in extenders A (70+/-7%), B (76+/-7%) and C (79+/-2%) was higher than that D (25+/-19%) and E (29+/-17%) (P<0.01). Sperm acrosomal integrity was better preserved in extenders B (86+/-4%) and C (83+/-4%) than in extenders A (54+/-13%), D (39+/-22%) and E (46+/-22%) (P<0.05). Post-thaw sperm longevity in extender A reduced from 69 to 16% during incubation (P<0.05) whereas only a slight decrease was observed in the other extenders after 4 h. In conclusion these data show that egg-yolk-Tris-Tes-glycerol based extender C containing Equex STM paste is optimal for freezing semen of Formosan Sika deer while egg-yolk-Tris-citric acid-glycerol based extender B containing Equex and extender C are superior in semen cryopreservation to others for Formosan Sambar deer.
authors.affiliation: Department of Veterinary Medicine, and Veterinary Teaching Hospital, College of Veterinary Medicine, National Chung-Hsing University, 250 Ko-Koung Road, Taichung City 402, Taiwan, ROC.
authors.forename: Feng-Pang
authors.initials: FP
authors.lastname: Cheng
authors.forename: Jui-Te
authors.initials: JT
authors.lastname: Wu
authors.forename: Jacky Peng-Wen
authors.initials: JP
authors.lastname: Chan
authors.forename: Jiunn-Shiow
authors.initials: JS
authors.lastname: Wang
authors.forename: Hang-Poung
authors.initials: HP
authors.lastname: Fung
authors.forename: Ben
authors.initials: B
authors.lastname: Colenbrander
authors.forename: Kwong-Chung
authors.initials: KC
authors.lastname: Tung
mesh_terms: D000177:Acrosome / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D000818:Animals; D002470:Cell Survival; D015925:Cryopreservation / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D003670:Deer; D006358:Hot Temperature; D008297:Male; D012662:Semen Preservation / Q000662:veterinary*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure
chemical_list: D003451:Cryoprotective Agents