title: Mapping critical biological motifs and biosynthetic pathways of heparan sulfate.
abstract: Heparan sulfate (HS) interacts with numerous proteins at the cell surface and orchestrates myriad biological events. Unraveling the mechanisms of these events at the molecular level calls for the structural analysis of these negatively charged and highly heterogeneous biopolymers. However, HS is often available only in small quantities, and the task of structural analysis necessitates the use of ultra-sensitive methods, such as mass spectrometry. Sequence heterogeneity within HS chains required us to identify critical functional groups and their spacing to determine structure-function relationships for HS. We carried out structural analysis of HS isolated from wild type, 3-OST-1, 3-OST-3A, or 3-OST-5 sulfotransferase-transduced Chinese hamster ovary cells and also from various tissues. In the context of tissue-specific HS, the data allowed us to map the biosynthetic pathways responsible for the placement of critical groups. As a means of determining the distance between critical groups within a motif, we determined the spacing of the rare GlcNAc-GlcA disaccharide sequence in the completely desulfated re-N-sulfated porcine intestinal heparin. These disaccharides are biosynthetic regulatory markers for 3-OST-1 modification and the partial structure of the antithrombin III binding site. They occur only at the distance of hexasaccharide, octasaccharide, decasaccharide, or dodecasaccharide. Thus this approach allowed us to map both the biosynthetic pathways for generating critical functional groups and their spacing within HS. Our new strategy removes two obstacles to rapid progress in this field of research.
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Roger
authors.initials: R
authors.lastname: Lawrence
authors.forename: Balagurunathan
authors.initials: B
authors.lastname: Kuberan
authors.forename: Miroslaw
authors.initials: M
authors.lastname: Lech
authors.forename: David L
authors.initials: DL
authors.lastname: Beeler
authors.forename: Robert D
authors.initials: RD
authors.lastname: Rosenberg
mesh_terms: D000818:Animals; D016466:CHO Cells; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D003412:Cricetulus; D004187:Disaccharides / Q000737:chemistry / Q000378:metabolism*; D005966:Glucuronidase / Q000378:metabolism; D006493:Heparin / Q000737:chemistry / Q000378:metabolism*; D006497:Heparan Sulfate / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism*; D013552:Swine
chemical_list: D004187:Disaccharides; D005966:Glucuronidase; D006493:Heparin; D006497:Heparan Sulfate; D011994:Recombinant Proteins