Selection in vitro of allosteric ribozymes.
The capacities of RNA for both catalysis and molecular recognition have been appreciated for some time. Recently, a number of studies have shown that these distinct functional classes may be combined to generate multipartite ribozymes in which the catalytic and ligand-binding elements act coordinately. In this chapter, we describe a procedure known as allosteric selection, in which an RNA catalyst in conjunction with a random sequence domain is subjected to iterative in vitro selection in order selectively to recover ligand-dependent ribozymes.
Roth
Adam
A
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT, USA.
Breaker
Ronald R
RR
D000494:Allosteric Regulation; D001483:Base Sequence; D017931:DNA Primers; D005818:Genetic Engineering / Q000379:methods; D007202:Indicators and Reagents; D007700:Kinetics; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D007202:Indicators and Reagents; D016337:RNA, Catalytic