title: Diversity of Y region at HML locus in a Saccharomyces cerevisiae strain isolated from a Sardinian wine.
abstract: Several mutations in genes involved in Saccharomyces mating type switching may affect the homothallic behaviour in wine yeasts. In this study the semi-homothallic (Hq) segregation of a flor wine yeast strain was analysed. We aimed to understand the molecular basis of this behaviour in a flor autochthonous strain, verifying the MAT locus status by a PCR-based HO gene disruption and sequencing of the Y region of the HML, HMR and MAT loci, after nested PCR. Presence of ORFs a1 and a2 in the Y region of the HML locus was found. At the ORF a2 at HML locus, a mutation in the stop codon was found, so the a2 ORF contains 33 more bases.
authors.affiliation: Dipartimento di Scienze Ambientali Agrarie e Biotecnologie Agro-Alimentari (DiSAABA), Sezione di Microbiologia Generale ed Applicata, Università di Sassari, Viale Italia 39, 07100 Sassari, Italy.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Pirino
authors.forename: Severino
authors.initials: S
authors.lastname: Zara
authors.forename: Giovanni
authors.initials: G
authors.lastname: Pinna
authors.forename: Giovanni Antonio
authors.initials: GA
authors.lastname: Farris
authors.forename: Marilena
authors.initials: M
authors.lastname: Budroni
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D017931:DNA Primers; D005800:Genes, Fungal / Q000235:genetics; D049770:Genes, Mating Type, Fungal; D014644:Genetic Variation; D007558:Italy; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000302:isolation & purification*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014920:Wine / Q000382:microbiology*
chemical_list: D017931:DNA Primers