title: High osmolarity glycerol (HOG) pathway-induced phosphorylation and activation of 6-phosphofructo-2-kinase are essential for glycerol accumulation and yeast cell proliferation under hyperosmotic stress.
abstract: In response to changes in the environment, yeast cells coordinate intracellular activities to optimize survival and proliferation. The transductions of diverse extracellular stimuli are exerted through multiple mitogen-activated protein kinase (MAPK) cascades. The high osmolarity glycerol (HOG) MAPK pathway is activated by increased environmental osmolarity and results in a rise of the cellular glycerol concentration to adapt the intracellular osmotic pressure. We studied the importance of the short time regulation of glycolysis under hyperosmotic stress for the survival and proliferation of yeast cells. A stimulation of the HOG-MAPK pathway by increasing the medium osmolarity through addition of salt or glucose to cultivated yeast leads to an activation of 6-phosphofructo-2-kinase (PFK2), which is accompanied by a complex phosphorylation pattern of the enzyme. An increase in medium osmolarity with 5% NaCl activates PFK2 3-fold over the initial value. This change in the activity is the result of a 4-fold phosphorylation of the enzyme mediated by protein kinases from the HOG-MAPK pathway. In the case of hyperosmolar glucose a 5-fold PFK2 activation was achieved by a single phosphorylation with protein kinase A near the carboxyl terminus of the protein on Ser(644) and an additional 5-fold phosphorylation within the same amino-terminal fragment as in the presence of salt. The effect of hyperosmolar glucose is the result of an activation of the Ras-cAMP pathway together with the HOG-MAPK pathway. The activation of PFK2 leads to an activation of the upper part of glycolysis, which is a precondition for glycerol accumulation. Yeast cells containing PFK2 accumulate three times more glycerol than cells lacking PFK2, which are not able to grow under hypertonic stress.
authors.affiliation: Department of Nephrology and Rheumatology, University Hospital Göttingen, Robert-Koch-Strasse 40, D-37075 Göttingen, Germany. dihazi@med.uni-goettingen.de
authors.forename: Hassan
authors.initials: H
authors.lastname: Dihazi
authors.forename: Renate
authors.initials: R
authors.lastname: Kessler
authors.forename: Klaus
authors.initials: K
authors.lastname: Eschrich
mesh_terms: D015153:Blotting, Western; D002455:Cell Division; D002470:Cell Survival; D003470:Culture Media; D000242:Cyclic AMP / Q000378:metabolism; D004789:Enzyme Activation; D005822:Genetic Vectors; D005947:Glucose / Q000378:metabolism; D019298:Glucose-6-Phosphate / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D006019:Glycolysis; D020935:MAP Kinase Signaling System; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009995:Osmosis; D025481:Phosphofructokinase-2 / Q000378:metabolism*; D010766:Phosphorylation; D010957:Plasmids / Q000378:metabolism; D017354:Point Mutation; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012694:Serine / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology
chemical_list: D003470:Culture Media; D012694:Serine; D019298:Glucose-6-Phosphate; D000242:Cyclic AMP; D025481:Phosphofructokinase-2; D014357:Trypsin; D005947:Glucose; D005990:Glycerol