title: Characterization of [(3)H]harmane binding to rat whole brain membranes.
abstract: This study investigates the binding of [(3)H]harmane to rat whole brain homogenates. Saturation studies revealed [(3)H]harmane labels a single, saturable, high-capacity population with high affinity. All the test compounds displaced [(3)H]harmane completely and in an apparently monophasic manner. The displacement profile of the test ligands indicated labeling of MAO-A. Given the high level of MAO-A binding, it is unlikely that a low-capacity I(2) site would be distinguishable from the total [(3)H]harmane population.
authors.affiliation: Psychopharmacology Unit, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK.
authors.forename: N J
authors.initials: NJ
authors.lastname: Anderson
authors.forename: E S J
authors.initials: ES
authors.lastname: Robinson
authors.forename: S M
authors.initials: SM
authors.lastname: Husbands
authors.forename: P
authors.initials: P
authors.lastname: Delagrange
authors.forename: D J
authors.initials: DJ
authors.lastname: Nutt
authors.forename: A L
authors.initials: AL
authors.lastname: Hudson
mesh_terms: D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D001923:Brain Chemistry; D002462:Cell Membrane / Q000378:metabolism*; D006247:Harmine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism*; D011869:Radioligand Assay; D051381:Rats; D014316:Tritium / Q000737:chemistry / Q000378:metabolism
chemical_list: D014316:Tritium; D006247:Harmine; C005010:harman