[Study on the fingerprints of the alkaloids in Sophora flavescens].
OBJECTIVE
To establish a method for HPLC fingerprint determination of the alkaloids in S. flavescens.


METHODS
RP-HPLC, linear gradient elution, LC/MS, etc. were used to determine the fingerprint and identify the main peaks in the HPLC fingerprint.


RESULTS
A satisfactory method for HPLC fingerprint determination of the alkaloids in S. flavescens. was established, and 5 peaks in the HPLC fingerprint were identified.


CONCLUSIONS
The perfect fingerprint can be obtained and the method can be used for quality control of S. flavescens.
Ma
Ren-ling
RL
Center for Instrumental Analysis, China Pharmaceutical University, Nanjing 210009, Jiangsu, China. marenling@sohu.com
Zhou
Hong-hua
HH
Yu
Xi-shui
XS
Sheng
Long-sheng
LS
D000470:Alkaloids / Q000008:administration & dosage / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D007267:Injections; D008970:Molecular Weight; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011807:Quinolizines; D029909:Sophora / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D000093842:Matrines
D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D011807:Quinolizines; C035933:sophocarpine; C037573:oxymatrine; D000093842:Matrines