EI-2346, a novel interleukin-1beta converting enzyme inhibitor produced by Streptomyces sp. E-2346. I. Taxonomy of producing strain, fermentation, isolation, physico-chemical properties, and biological properties.
EI-2346, a novel interleukin-1beta converting enzyme (ICE) inhibitor, was isolated from the culture broths of Streptomyces sp. E-2346. EI-2346 selectively inhibited the human recombinant ICE activity with an IC50 value of 3.9 microM, without inhibiting elastase and cathepsin B. EI-2346 also inhibited mature interleukin-1beta secretion from THP-1 cells induced by LPS with an IC50 value of 5.2 microM.
Koizumi
Fumito
F
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahimachi, Machida-shi, Tokyo 194-8533, Japan. fumito.koizumi@kyowa.co.jp
Hasegawa
Atsuhiro
A
Ochiai
Keiko
K
Ando
Katsuhiko
K
Kondo
Hidemasa
H
Yoshida
Mayumi
M
Matsuda
Yuzuru
Y
Nakanishi
Satoshi
S
D061945:Caspase Inhibitors; D002460:Cell Line; D003470:Culture Media; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D005285:Fermentation; D015394:Molecular Structure; D009285:Naphthoquinones / Q000737:chemistry / Q000494:pharmacology; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors; D013302:Streptomyces / Q000145:classification* / Q000378:metabolism
D061945:Caspase Inhibitors; D003470:Culture Media; C484082:EI-2346; D004791:Enzyme Inhibitors; D009285:Naphthoquinones; D011994:Recombinant Proteins