Mass-tag technology for monitoring of protein kinase activity using mass spectrometry.
Monitoring of intracellular protein kinase activity is very important for fields involving diagnosis and drug screening. However, current methods, such as radiometry using (32)P, or ELISA, are laborious and time-consuming. We have developed high-throughput assay system of protein kinase activity using mass-tagged substrate peptide probes and mass spectrometry. This assay system can easily evaluate target kinase activity and will potentially be able to simultaneously profile many protein kinase activities.
Sonoda
Tatsuhiko
T
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Shigaki
Syuhei
S
Nagashima
Takeyuki
T
Okitsu
Osamu
O
Kita
Yasuhiro
Y
Murata
Masaharu
M
Katayama
Yoshiki
Y
D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D011494:Protein Kinases / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
D010455:Peptides; D011494:Protein Kinases