Protective effect of taurine on the detachment of cultured cardiac fibroblasts from the substratum induced by calcium depletion.
Removal of Ca2+ from the incubation medium of cultured rat cardiac fibroblasts causes cellular morphological changes, such as the formation of blebs, the ballooning of the cell membrane and the detachment from the culture dish. A 24 hr preincubation with 20 mM taurine blocked the Ca2+ depletion-induced detachment of the cardiac fibroblasts. However, taurine treatment did not prevent other morphological changes induced by Ca2+ depletion. The data suggest that taurine plays an important role in cell adhesion in the heart.
Azuma
M
M
Department of Clinical Evaluation of Medicines and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, 565-0871 Osaka, Japan. azuma@phs.osaka-u.ac.jp
Takahashi
K
K
Kimura
A
A
Matsuda
T
T
Schaffer
S W
SW
Azuma
J
J
D000818:Animals; D002118:Calcium / Q000494:pharmacology* / Q000502:physiology; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D009206:Myocardium / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D013654:Taurine / Q000494:pharmacology*
D002121:Calcium Channel Blockers; D013654:Taurine; D002118:Calcium