Administration of hyaluronic acid and chondroitin-4-sulfate limits endogenous antioxidant depletion and reduces cell damage in experimental acute pancreatitis.
Several reports have described a loss of endogenous antioxidants and molecular oxidative damage during acute pancreatitis. Since hyaluronic acid and chondroitin-4-sulfate possess antioxidant properties, the effect of the administration of these glycosaminoglycans in a cerulein-induced acute pancreatitis in rats was investigated. Cerulein administration produced pancreatic edema and a marked increase in serum lipase and amylase activity; induced a severe depletion of reduced glutathione, catalase, and superoxide dismutase levels; primed lipid peroxidation; and promoted neutrophil intervention. Intraperitoneal pretreatment of rats with hyaluronic acid or chondroitin-4-sulfate or with both compounds ameliorated pancreatic cell conditions; restored the endogenous antioxidants reduced glutathione, catalase and superoxide dismutase; limited cell membrane peroxidation; and reduced neutrophil activation. Our data confirm the antioxidant activity of these 2 glycosaminoglycans.
Campo
Giuseppe M
GM
Department of Biochemical, Physiological, and Nutritional Sciences, School of Medicine, University of Messina, Policlinico Universitario, Messina, Italy. gcampo@unime.it
Avenoso
Angela
A
Campo
Salvatore
S
Ferlazzo
Alida M
AM
Calatroni
Alberto
A
D000208:Acute Disease; D000681:Amylases / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000627:therapeutic use*; D002809:Chondroitin Sulfates / Q000627:therapeutic use*; D004487:Edema / Q000188:drug therapy; D006820:Hyaluronic Acid / Q000627:therapeutic use*; D008049:Lipase / Q000378:metabolism; D008297:Male; D010195:Pancreatitis / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D000975:Antioxidants; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D009195:Peroxidase; D008049:Lipase; D000681:Amylases