Melanocins A, B and C, new melanin synthesis inhibitors produced by Eupenicillium shearii. I. Taxonomy, fermentation, isolation and biological properties.
New melanin synthesis inhibitors, melanocins A, B and C, were isolated from the fermentation broth and mycelium extract of Eupenicillium shearii F80695. Melanocin A, an isocyanide compound, inhibited mushroom tyrosinase and melanin biosynthesis of B16 melanoma cells with IC50 value of 9.0 nM and MIC value of 0.9 microM, respectively. Melanocin A also inhibited growth of Streptomyces bikiniensis. While, the structurally very related but non-isocyanide compounds melanocins B and C did not show inhibitory activity in these assays. Melanocins A, B and C showed potent antioxidant activity with scavenging activity of DPPH radical and superoxide anion radical.
Kim
Jong-Pyung
JP
Korea Research Institute of Bioscience and Biotechnology, Yuseong, Daejeon 305-600, Korea.
Kim
Byoung-Kwon
BK
Yun
Bong-Sik
BS
Ryoo
In-Ja
IJ
Lee
Choong Hwan
CH
Lee
In-Kyoung
IK
Kim
Won-Gon
WG
Lee
Sangku
S
Pyun
Yu-Ryang
YR
Yoo
Ick-Dong
ID
D000363:Agaricales / Q000201:enzymology; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology; D001203:Ascomycota / Q000145:classification / Q000378:metabolism*; D002074:Butanones / Q000302:isolation & purification / Q000494:pharmacology; D003486:Cyanides / Q000302:isolation & purification / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D005559:Formamides / Q000302:isolation & purification / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D008543:Melanins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors; D013302:Streptomyces / Q000187:drug effects / Q000254:growth & development
D000900:Anti-Bacterial Agents; D002074:Butanones; D003486:Cyanides; D004791:Enzyme Inhibitors; D005559:Formamides; D016166:Free Radical Scavengers; D008543:Melanins; C484096:melanocin A; C484095:melanocin B; C484097:melanocin C; D014442:Monophenol Monooxygenase