[Histo-morphological changes of eustachian tube of guinea pigs with effusion after being treated by pulmonary surfactant].
OBJECTIVE
To study the effect of pulmonary surfactant on otitis media with effusion in guinea pigs to find a new way to manage otitis media with effusion.


METHODS
Nonviable heat-killed pneumococci (HKP) solution was inoculated into the middle ear cavity in guinea pigs via a transeardrum approach to set up a model of otitis media with effusion in guinea pigs. Seven days after being injected with pulmonary surfactant (PS) by transeardrum approach, ABR threshold and histomorphological changes of eustachian tube mucosa of guinea pigs were examined by light microscopy and scanning microscopy.


RESULTS
Five days following inoculation of HKB serous effusion were present in the middle ear cavity of guinea pigs, but disappearance of light cone. Response (mean +/- s) threshold raised from (14.0 +/- 3.1) dB to (45.0 +/- 5.7) dB. The eustachian tube mucosa was thickened and lined eosin-stained structureless matter over mucosa, while cilia of eustachian tube mucosa irregularly arranged. Seven days after being treated by PS, serous effusion of tympanum was reduced or disappeared, and response threshold decreased from (45.0 +/- 5.7) dB to (23.5 +/- 6.3) dB. There was significantly difference between them (P < 0.001). Eustachian tube mucosa was thinned, Cilia of eustachian tube mucosa regularly arranged to the nasopharynx.


CONCLUSIONS
Pulmonary surfactant plays a important role in otitis media with effusion of guinea pigs.
Ma
Zhao-xin
ZX
Department of Otorhinolaryngology, Yueyang Hospital, Traditional Chinese & Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China. Mazhaoxincn@yahoo.com.cn
Chen
Xu-hui
XH
Li
Ming
M
Huang
Ping
P
Cao
Yi
Y
Cai
Jian-feng
JF
D000818:Animals; D005064:Eustachian Tube / Q000473:pathology*; D016057:Evoked Potentials, Auditory, Brain Stem; D005260:Female; D006168:Guinea Pigs; D008297:Male; D010034:Otitis Media with Effusion / Q000382:microbiology / Q000473:pathology* / Q000628:therapy; D011663:Pulmonary Surfactants / Q000494:pharmacology* / Q000627:therapeutic use; D011897:Random Allocation; D013296:Streptococcus pneumoniae
D011663:Pulmonary Surfactants