title: Stimulation of interleukin-10 production by acidic beta-lactoglobulin-derived peptides hydrolyzed with Lactobacillus paracasei NCC2461 peptidases.
abstract: We have previously demonstrated that Lactobacillus paracasei NCC2461 may help to prevent cow's milk allergy in mice by inducing oral tolerance to beta-lactoglobulin (BLG). To investigate the mechanisms involved in this beneficial effect, we examined the possibility that L. paracasei induces tolerance by hydrolyzing BLG-derived peptides and liberating peptides that stimulate interleukin-10 (IL-10) production. L. paracasei peptidases have been shown to hydrolyze tryptic-chymotryptic peptides from BLG, releasing numerous small peptides with immunomodulating properties. We have now shown that acidic tryptic-chymotryptic peptides stimulate splenocyte proliferation and gamma interferon (IFN-gamma) production in vitro. Hydrolysis of these peptides with L. paracasei peptidases repressed the lymphocyte stimulation, up-regulated IL-10 production, and down-regulated IFN-gamma and IL-4 secretion. L. paracasei NCC2461 may therefore induce oral tolerance to BLG in vivo by degrading acidic peptides and releasing immunomodulatory peptides stimulating regulatory T cells, which function as major immunosuppressive agents by secreting IL-10.
authors.affiliation: Dairy Research Centre STELA, Département des Sciences des Aliments et de Nutrition, Université Laval, Québec, Québec, Canada G1K 7P4. guenolee_prioult@hotmail.com
authors.forename: Guénolée
authors.initials: G
authors.lastname: Prioult
authors.forename: Sophie
authors.initials: S
authors.lastname: Pecquet
authors.forename: Ismail
authors.initials: I
authors.lastname: Fliss
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D002455:Cell Division / Q000276:immunology; D005260:Female; D006868:Hydrolysis; D007108:Immune Tolerance / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism*; D007778:Lactobacillus / Q000201:enzymology* / Q000276:immunology*; D007782:Lactoglobulins / Q000276:immunology* / Q000378:metabolism*; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010447:Peptide Hydrolases / Q000378:metabolism; D013154:Spleen / Q000276:immunology
chemical_list: D001426:Bacterial Proteins; D007782:Lactoglobulins; D016753:Interleukin-10; D007371:Interferon-gamma; D010447:Peptide Hydrolases