Approaches to identify, clone, and express symbiont bioactive metabolite genes.
This review discusses approaches to identify, clone, and express bioactive metabolite genes from symbionts of marine invertebrates. Criteria for proving symbiotic origin of bioactive metabolites are presented, followed by a comprehensive, practically-oriented overview of techniques to be applied. The Bugula neritina/Endobugula sertula association is used as a primary example, but other symbioses are discussed. Thirty-six compounds are presented and 111 references are cited.
Hildebrand
Mark
M
Scripps Institution of Oceanography, Marine Biology Research Division; Center for Marine Biotechnology and Biomedicine; and UCSD Cancer Center, University of California, San Diego, La Jolla, California 92093, USA.
Waggoner
Laura E
LE
Lim
Grace E
GE
Sharp
Katherine H
KH
Ridley
Christian P
CP
Haygood
Margo G
MG
D000818:Animals; D001685:Biological Factors / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D005827:Genetics, Microbial; D007448:Invertebrates / Q000737:chemistry / Q000235:genetics; D008386:Marine Biology; D008954:Models, Biological; D015394:Molecular Structure; D017422:Sequence Analysis, DNA; D013559:Symbiosis / Q000235:genetics
D001685:Biological Factors