title: Cell growth on the porous sponges prepared from poly(depsipeptide-co-lactide) having various functional groups.
abstract: In tissue engineering, excellent biodegradable materials are desired as temporary scaffolds to support cell growth and disappear with the progress of tissue regeneration. We previously synthesized biodegradable poly(depsipeptide-co-lactide), poly[(Glc-Asp)-co-LA] and poly[(Glc-Lys)-co-LA], having reactive side-chain groups. Then, the effects of reactive and ionic side-chain groups on cell attachment and growth were investigated using co-polymer films with various amounts of carboxyl or amino groups. In this study, to evaluate the utility of these co-polymers as functional scaffolds for tissue regeneration, 3-dimensional porous sponges were prepared by freeze-drying method and the effects of reactive and ionic side-chain groups on cell growth and degradation behavior were investigated using co-polymer sponges with various amounts of carboxyl or amino groups. Good cell growth was observed on the co-polymer sponges. During cell culture, the co-polymer sponges exhibited various degradation rates related to the depsipeptide unit content. Three-dimensional biodegradable polymer matrices with reactive surface, controllable degradation behavior and good cell growth were successfully prepared using these co-polymers. Such kinds of co-polymer matrices are good candidate for scaffold for tissue engineering.
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering & High Technology Research Center, Kansai University, Suita, Osaka 564-8680, Japan. yohya@ipcku.kansai-u.ac.jp
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ohya
authors.forename: Hideaki
authors.initials: H
authors.lastname: Matsunami
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Ouchi
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001224:Aspartic Acid / Q000737:chemistry; D001673:Biodegradation, Environmental; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D047630:Depsipeptides; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D006016:Glycolates / Q000737:chemistry; D008239:Lysine / Q000737:chemistry; D051379:Mice; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D016062:Porosity; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods
chemical_list: D047630:Depsipeptides; D006016:Glycolates; D010456:Peptides, Cyclic; D011091:Polyesters; D011108:Polymers; C476738:poly(depsipeptide-co-lactide); C031149:glycolic acid; D001224:Aspartic Acid; C033616:poly(lactide); D008239:Lysine