title: Serum protein profile alterations in hemodialysis patients.
abstract: BACKGROUND
  Serum protein profiling patterns can reflect the pathological state of a patient and therefore may be useful for clinical diagnostics. Here, we present results from a pilot study of proteomic expression patterns in hemodialysis patients designed to evaluate the range of serum proteomic alterations in this population.
  
  
  METHODS
  Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) was used to analyze serum obtained from patients on periodic hemodialysis treatment and healthy controls. Serum samples from patients and controls were first fractionated into six eluants on a strong anion exchange column, followed by application to four array chemistries representing cation exchange, anion exchange, metal affinity and hydrophobic surfaces. A total of 144 SELDI-TOF-MS spectra were obtained from each serum sample.
  
  
  RESULTS
  The overall profiles of the patient and control samples were consistent and reproducible. However, 30 well-defined protein differences were observed; 15 proteins were elevated and 15 were decreased in patients compared to controls. Serum from 1 patient exhibited novel protein peaks suggesting possible additional changes due to a secondary disease process.
  
  
  CONCLUSIONS
  SELDI-TOF-MS demonstrated consistent serum protein profile differences between patients and controls. Similarity in protein profiles among dialysis patients suggests that patient physiological responses to end-stage renal disease and/or dialysis therapy have a major effect on serum protein profiles.
authors.affiliation: Lawrence Livermore National Laboratory, Livermore, CA 94550, USA.
authors.forename: Richard G
authors.initials: RG
authors.lastname: Langlois
authors.forename: James E
authors.initials: JE
authors.lastname: Trebes
authors.forename: Enrique A
authors.initials: EA
authors.lastname: Dalmasso
authors.forename: Yong
authors.initials: Y
authors.lastname: Ying
authors.forename: Robert W
authors.initials: RW
authors.lastname: Davies
authors.forename: Mario P
authors.initials: MP
authors.lastname: Curzi
authors.forename: Bill W
authors.initials: BW
authors.lastname: Colston
authors.forename: Kenneth W
authors.initials: KW
authors.lastname: Turteltaub
authors.forename: Julie
authors.initials: J
authors.lastname: Perkins
authors.forename: Brett A
authors.initials: BA
authors.lastname: Chromy
authors.forename: Megan W
authors.initials: MW
authors.lastname: Choi
authors.forename: Gloria A
authors.initials: GA
authors.lastname: Murphy
authors.forename: J Patrick
authors.initials: JP
authors.lastname: Fitch
authors.forename: Sandra L
authors.initials: SL
authors.lastname: McCutchen-Maloney
mesh_terms: D000328:Adult; D001798:Blood Proteins / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis; D051437:Renal Insufficiency / Q000097:blood* / Q000628:therapy*
chemical_list: D001798:Blood Proteins