Prophylactic action of melatonin against cyclophosphamide-induced oxidative stress in mice.
The present study investigated the prophylactic influence of melatonin against cyclophosphamide-induced oxidative stress in mouse tissues. Lipid peroxidation, reduced glutathione (GSH), glutathione disulphide (GSSG), glutathione peroxidase (GSH-Px) and serum phosphatase levels were analyzed in brain, spleen liver, lungs, kidney and testes. Fifteen days oral administration with melatonin (0.1 mg/kg bw per day) before treatment checked the augmentation of the level of lipid peroxidation, blood GSSG and acid phosphatase caused by an acute treatment with a radiomimetic drug, cyclophosphamide (75 mg/kg bw). Cyclophosphamide-induced depletion in the level of GSH, GSH-Px and alkaline phosphatase was made up statistically significant by chronic melatonin administration given orally. The results indicate the antioxidative properties of melatonin resulting into its prophylactic property against the cyclophosphamide-induced biochemical alterations. The finding support the idea that melatonin is a potent free-radical scavenger and antioxidant.
Manda
K
K
Laboratory of Neurobiology and Aging, Department of Zoology, University of Rajasthan, Jaipur-302 004, India.
Bhatia
A L
AL
D000469:Alkaline Phosphatase / Q000493:pharmacokinetics; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D003520:Cyclophosphamide / Q000633:toxicity*; D005978:Glutathione / Q000493:pharmacokinetics; D019803:Glutathione Disulfide / Q000493:pharmacokinetics; D005979:Glutathione Peroxidase / Q000493:pharmacokinetics; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008168:Lung / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D013154:Spleen / Q000187:drug effects / Q000201:enzymology; D013737:Testis / Q000187:drug effects / Q000201:enzymology
D000975:Antioxidants; D003520:Cyclophosphamide; D005979:Glutathione Peroxidase; D000469:Alkaline Phosphatase; D005978:Glutathione; D008550:Melatonin; D019803:Glutathione Disulfide