The impact of Ti-plasmid-derived gene vectors on the study of the mechanism of action of phytohormones.
The molecular basis of tumor formation on dicotyledonous plants by Agrobacterium relies on the transfer to the plant cell of a unique segment of bacterial DNA, the T-DNA. The T-DNA contains genes that are active in the plant cell and encode hormone biosynthetic enzymes, or proteins that deregulate the cell's response to phytohormones. Study of this process has yielded not only knowledge of how alterations in phytohormone homeostasis can affect plant cell growth, but also has provided the essential tools to study phytohormone signaling in transgenic plants. Furthermore, T-DNA insertion into the plant genome forms the basis of gene tagging, a versatile method for isolating genes involved in phytohormone signal transduction and action.
Walden
R
R
Max-Planck-Institut für Zuchtungsforschung, Cologne, Germany. Walden@mpiz-koeln.mpg.de
Reiss
B
B
Koncz
C
C
Schell
J
J