title: Induction of the apoptosis of HL-60 promyelocytic leukemia cells by Eurya emarginata.
abstract: This study examined the inhibitory effect of Eurya emarginata extracts on the growth of cancer cells. The ethylacetate (EtOAc) fraction of the E. emarginata leaves markedly inhibited the growth of leukemia cells including HL-60. When the HL-60 cells were treated with its EtOAc fraction, DNA fragmentation and sub-G1 hypodiploid cells were observed. Moreover, the EtOAc fraction of E. emarginata reduced the c-Myc and Bcl-2 protein and mRNA levels in a time-dependent manner, whereas the Bax protein and mRNA expression levels were slightly increased. The results suggest that the inhibitory effect of E. emarginata on the growth of HL-60 appears to arise from the induction of apoptosis via the down-regulation of Bcl-2 and c-Myc expression with an increase in the Bax expression level.
authors.affiliation: Department of Medicine, College of Medicine, Cheju National University, Jeju, South Korea.
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Park
authors.forename: Hong Chul
authors.initials: HC
authors.lastname: Yang
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Moon
authors.forename: Nam Ho
authors.initials: NH
authors.lastname: Lee
authors.forename: Se Jae
authors.initials: SJ
authors.lastname: Kim
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Kang
authors.forename: Young Ki
authors.initials: YK
authors.lastname: Lee
authors.forename: Deok Bae
authors.initials: DB
authors.lastname: Park
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Yoo
authors.forename: Hee Kyoung
authors.initials: HK
authors.lastname: Kang
mesh_terms: D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002455:Cell Division / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000188:drug therapy*; D010936:Plant Extracts / Q000494:pharmacology*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000187:drug effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000187:drug effects; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D051028:bcl-2-Associated X Protein
chemical_list: D016588:Anticarcinogenic Agents; C494826:BAX protein, human; D010936:Plant Extracts; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein