Expression of three carnitine palmitoyltransferase-I isoforms in 10 regions of the rat brain during feeding, fasting, and diabetes.
Inhibition of carnitine palmitoyltransferase-I (CPT-I) activity in the brain has been shown to decrease food intake in rats. We examined the expression of mRNA encoding all three known CPT-I isoforms (alpha, beta, and gamma in 10 different major regions of the rat brain in normal, chow-fed rats, in fasting rats, and in insulin-dependent diabetic rats. Compared with the effects of fasting and diabetes on CPT-I mRNA in the liver and heart, there was either less effect or no effect depending on the particular brain region examined. These results suggest that the regulation of CPT-I mRNA levels is different in the brain than in other tissues. A surprising result of this study was the discovery of very high, unique expression of CPT-Ibeta (the muscle isoform) in the cerebellum.
Lavrentyev
Eduard N
EN
Department of Pharmacology, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA. elavrentyev@utmem.edu
Matta
Shannon G
SG
Cook
George A
GA
D000821:Animal Feed; D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000201:enzymology* / Q000502:physiology; D002334:Carnitine O-Palmitoyltransferase / Q000096:biosynthesis* / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D004790:Enzyme Induction; D005215:Fasting / Q000378:metabolism*; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
D017931:DNA Primers; D007527:Isoenzymes; D012333:RNA, Messenger; D002334:Carnitine O-Palmitoyltransferase