TNF-alpha and IL-4 regulate expression of fractalkine (CX3CL1) as a membrane-anchored proadhesive protein and soluble chemotactic peptide on human fibroblasts.
The CX(3)C chemokine, fractalkine (FKN, CX(3)CL1), has multiple functions and exists as two distinct forms, a membrane-anchored protein and a soluble chemotactic peptide that cleaves from the cell surface FKN. In this study, we first demonstrated the expression of FKN in tumor necrosis factor (TNF)-alpha- and interleukin (IL)-4-stimulated human fibroblasts. The induction of FKN was observed for both forms. We also demonstrated monocyte chemotactic activity in the culture supernatant from the fibroblasts stimulated with these cytokines. These results suggest that TNF-alpha- and IL-4-stimulated fibroblasts may play an important role in accumulation of monocytes at inflammatory sites.
Yoshikawa
Mamoru
M
Department of Allergy and Immunology, National Research Institute for Child Health and Development, 3-35-31 Taishidou, Setagaya-ku, Tokyo 154-8567, Japan.
Nakajima
Toshiharu
T
Matsumoto
Kenji
K
Okada
Naoko
N
Tsukidate
Toshiharu
T
Iida
Makoto
M
Otori
Nobuyoshi
N
Haruna
Shin-Ichi
S
Moriyama
Hiroshi
H
Imai
Toshio
T
Saito
Hirohisa
H
D015815:Cell Adhesion Molecules; D054428:Chemokine CX3CL1; D020523:Chemokines, CX3C / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D002633:Chemotaxis; D005347:Fibroblasts / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology*; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D009000:Monocytes / Q000166:cytology; D012995:Solubility; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
C515538:CX3CL1 protein, human; D015815:Cell Adhesion Molecules; D054428:Chemokine CX3CL1; D020523:Chemokines, CX3C; D008565:Membrane Proteins; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4