title: Expression of a novel secreted factor, Seraf indicates an early segregation of Schwann cell precursors from neural crest during avian development.
abstract: The neural crest gives rise to glial cells in the peripheral nervous system. Among the peripheral glia, Schwann cells form the myelin often wrapping the peripheral axons. Compared to other crest-derived cell lineages such as neurons, the analysis of fate determination and subsequent differentiation of Schwann cells is not well advanced, partly due to the lack of early markers of this phenotype. In this study, we have identified a gene, uniquely expressed in avian embryo Schwann cell precursors, which encodes a novel secreted factor, designated Seraf (Schwann cell-specific EGF-like repeat autocrine factor). Expression of Seraf and P0 delineates the earliest phase of Schwann cell differentiation. Seraf binds to neural crest cells and Schwann cells, and affects the distribution of Schwann cells, when introduced to chicken embryos during neural crest migration. Our results suggest an autocrine function of Seraf and provide a significant step to understand the developmental processes of Schwann cell lineage.
authors.affiliation: Center for Translational and Advanced Animal Research on Human Diseases, Division of Developmental Neuroscience, Graduate School of Medicine, Tohoku University, Sendai, Miyagi 980-8575, Japan. wakasama@mail.cc.tokohu.ac.jp
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Wakamatsu
authors.forename: Noriko
authors.initials: N
authors.lastname: Osumi
authors.forename: James A
authors.initials: JA
authors.lastname: Weston
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics*; D002645:Chickens; D003001:Cloning, Molecular; D003370:Coturnix; D017931:DNA Primers; D008969:Molecular Sequence Data; D009420:Nervous System / Q000166:cytology*; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D012583:Schwann Cells / Q000166:cytology* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D011506:Proteins