[Studies on callus induced from leaves and plantlets regeneration of the traditional Chinese medicine Chrysanthemum morifolium].
OBJECTIVE
To set up the optimums for the leaf tissue culture of Chrysanthemum morifolium cultivated in Anhui Province.


METHODS
Leaves were cut and cultured onto the same kind of media on different sides and defferent kinds of media on the same side, and induced to form the whole plantlet.


CONCLUSIONS
All the media used could induce callus, while the results of callus redifferentiation were very distinct. The media with NAA 0.1 mg.L-1 + 6-BA 0.1-1.0 mg.L-1 were much better than others when callus were induced. The MS medium with 6-BA 2.0 mg.L-1 and NAA 0.5 mg.L-1 was the preferable medium for the bud sprouting. The regenerated plantlets produced variation of leaf shape.
Xue
Jian-ping
JP
Department of Biology, Huaibei Coal Industry Teacher's College, Huabei 235000, Anhui, China. xueJP2000@yahoo.com.cn
Yu
Miao
M
Zhang
Ai-min
AM
D031202:Chrysanthemum / Q000254:growth & development*; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D018515:Plant Leaves / Q000254:growth & development; D010946:Plants, Medicinal / Q000254:growth & development*
D003470:Culture Media