title: Anti-inflammatory flavonoids and pterocarpanoid from Crotalaria pallida and C. assamica.
abstract: One new isoflavone, 5,7,4'-trihydroxy-2'-methoxyisoflavone (3) and seven, and four known compounds were isolated from the barks of Crotalaria pallida and the seeds of C. assamica, respectively. The known compounds, apigenin (1) and 2'-hydroxygenistein (2), isolated from C. pallida, showed significant concentration-dependent inhibitory effects on the release of beta-glucuronidase and lysozyme from rat neutrophils in response to formyl-Met-Leu-Phe/cytochalasin B (fMLP/CB) with IC(50) values of 2.8+/-0.1 and 17.7+/-1.9, and 5.9+/-1.4 and 9.7+/-3.5 microM, respectively. The known compounds, daidzein (4) and 2'-hydroxydaidzein (6), isolated from C. pallida, inhibited of the release of lysozyme and beta-glucuronidase from rat neutrophils in response to fMLP/CB with IC(50) values of 26.3+/-5.5 and 13.7+/-2.6 microM, respectively. Compounds 1 and 4 also showed significant concentration-dependent inhibitory effects on superoxide anion generation in rat neutrophils stimulated with fMLP/CB with IC(50) values of 3.4+/-0.3 and 25.1+/-5.0 microM, respectively. Compounds 1 and 5, previously isolated from C. pallida, showed the inhibition of NO production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and LPS/interferon-gamma (IFN-gamma)-stimulated N9 microglial cells with IC(50) values of 10.7+/-0.1 and 13.9+/-1.1 microM, respectively. Flavonoids, suppressed chemical mediators in inflammatory cells, may have value in treatment and prevention of central and peripheral inflammatory diseases associated with excess production of chemical mediators.
authors.affiliation: Faculty of Fragrance and Cosmetics, Kaohsiung Medical University, Kaohsiung, Taiwan 807, Republic of China.
authors.forename: Horng-Huey
authors.initials: HH
authors.lastname: Ko
authors.forename: Jing-Ru
authors.initials: JR
authors.lastname: Weng
authors.forename: Lo-Ti
authors.initials: LT
authors.lastname: Tsao
authors.forename: Ming-Hong
authors.initials: MH
authors.lastname: Yen
authors.forename: Jih-Pyang
authors.initials: JP
authors.lastname: Wang
authors.forename: Chun-Nan
authors.initials: CN
authors.lastname: Lin
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D029864:Crotalaria / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005966:Glucuronidase / Q000037:antagonists & inhibitors; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D015394:Molecular Structure; D009113:Muramidase / Q000037:antagonists & inhibitors; D009504:Neutrophils / Q000187:drug effects; D024301:Plant Bark / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D012639:Seeds / Q000737:chemistry
chemical_list: C490867:5,7,4'-trihydroxy-2'-methoxyisoflavone; D000894:Anti-Inflammatory Agents, Non-Steroidal; D005419:Flavonoids; D007529:Isoflavones; D009113:Muramidase; D005966:Glucuronidase