Functional characterization of sodium- and chloride-dependent taurine transport in human keratinocytes.
In the skin, taurine acts as an important osmolyte required for keratinocyte hydration. It has antioxidant effects, protects cells from UV-induced stress and has effects on cell proliferation, inflammation and collagenogenesis. This study was performed to find and characterize functionally a taurine transport system in keratinocytes and to establish a cell culture model for skin taurine transport studies. Uptake of [(3)H]taurine was studied both in the human adult low calcium high temperature (HaCaT) cell line and in human native epidermal keratinocytes. Uptake of [(3)H]taurine in HaCaT cells was strictly dependent on extracellular sodium and chloride. The taurine uptake rate was saturable and indicated participation of a single transport system with kinetic parameters of Kt = 5.1 +/- 0.2 microm and Vmax = 320.5 +/- 2.8 pmol/10 min per mg of protein. Uptake was strongly inhibited by beta-amino acids (taurine, beta-alanine, hypotaurine, beta-guanidinopropionic acid), whereas alpha- and gamma-amino acids had little or no effect. Taurine uptake in normal keratinocytes was very similar to that in HaCaT cells with respect to substrate specificity and affinity. We conclude that keratinocytes express the Na(+) and Cl(-) dependent, high-affinity taurine transporter. This system accepts beta- and certain gamma-amino acids as substrates.
Grafe
Franziska
F
Membrane Transport Group, Biozentrum of the Martin-Luther-University Halle-Wittenberg, Halle, Germany.
Wohlrab
Wolfgang
W
Neubert
Reinhard H
RH
Brandsch
Matthias
M
D001692:Biological Transport / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D012965:Sodium Chloride / Q000378:metabolism*; D013654:Taurine / Q000378:metabolism*
D013654:Taurine; D012965:Sodium Chloride