[Isolation and structure identification of lignans from pine needles of pinus massoniana Lamb].
OBJECTIVE
To study the chemical constituents from the water-extracts of pine needles of Pinus massoniana Lamb.


METHODS
Chromatographic techniques were used to separate and purify compounds. Their physico-chemical properties and spectral data (UV, IR, MS, 1H-1H, 13C-1H NMR, DEPT, HMBC etc.) were used to elucidate the structures.


RESULTS
Three lignans were isolated from the n-BuOH fraction of water-extracts. Their structures were identified as (7S,8R)-3',4,9'-tridihydroxy-4-methoxy- 9-O-shikimoyl-7,8-dihydrobenzofuran-1'-propylneolignan (massonianoid A, I), (7S,8R)-4,9-dihydroxy-3,3'-dimethoxy-7,8- dihydrobenzofuran-1'-propylneolignan (II) and 4,4',8-trihydroxy-4,4'-dimethoxy-9-lignanolide (III).


CONCLUSIONS
Compound I is a new compound. While II and III were isolated from this plant for the first time.
Feng
Wei-sheng
WS
Henan College of Traditional Chinese Medicine, Zhengzhou 450008, China. fwsh@371.net
Zheng
Xiao-ke
XK
Wang
Yan-zhi
YZ
Bi
Yue-feng
YF
D005663:Furans / Q000737:chemistry / Q000302:isolation & purification; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D028223:Pinus / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
C489700:(7S,8R)-3',4,9'-tridihydroxy-4-methoxy- 9-O-shikimoyl-7,8-dihydrobenzofuran-1'-propylneolignan; D005663:Furans; D017705:Lignans; C020436:nortrachelogenin