Detection by real-time PCR of the Pfcrt T76 mutation, a molecular marker of chloroquine-resistant Plasmodium falciparum strains.
An efficient and fast diagnostic assay was developed to detect the point mutation involved in the resistance of Plasmodium falciparum to chloroquine. The test, based upon a real-time PCR principle and performed with the LightCycler system, was carried out on venous blood isolates from 221 cases of falciparum malaria. The assay provided a quick, sensitive and reliable detection of the T76 Pfcrt mutation and the chloroquine resistance. The results of this study suggest that this LightCycler-based technique could be used to optimise the treatment of imported uncomplicated falciparum malaria, and also for the molecular surveillance of drug resistance among imported cases of this disease.
Vessière
Aurélia
A
Service de Parasitologie, Molecular Diagnosis Unit, Centre Hospitalier Universitaire Rangueil, 31403 Toulouse 4, France.
Berry
Antoine
A
Fabre
Richard
R
Benoit-Vical
Françoise
F
Magnaval
Jean-François
JF
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D002738:Chloroquine / Q000494:pharmacology*; D016054:DNA, Protozoan / Q000032:analysis; D004351:Drug Resistance / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D016778:Malaria, Falciparum / Q000175:diagnosis / Q000469:parasitology; D008565:Membrane Proteins / Q000235:genetics*; D026901:Membrane Transport Proteins; D021261:Parasitic Sensitivity Tests; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000302:isolation & purification; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D015800:Protozoan Proteins; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000962:Antimalarials; D016054:DNA, Protozoan; D005819:Genetic Markers; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C416369:PfCRT protein, Plasmodium falciparum; D015800:Protozoan Proteins; D002738:Chloroquine