title: The effect of CAPE on lipid peroxidation and nitric oxide levels in the plasma of rats following thermal injury.
abstract: Both experimental and clinical studies have shown that oxygen-derived free radicals rise in the plasma after thermal injury and participate in the pathogenesis of tissue damage. Hence, various antioxidant molecules have been used in treatment of burn injury both experimentally and clinically. Caffeic acid phenethyl ester (CAPE), an active component of propolis from honeybee hives, is known to have potent antioxidant property. The purpose of the present study was to investigate the effects of CAPE on oxidative stress in plasma of burned rats. Experiment was designed in three groups of rats with 20% full-thickness burn: (a) sham burn (n = 7); (b) burn only (n = 22); (c) burn + treatment with CAPE (n = 22). Plasma levels of malondialdehyde (MDA), nitric oxide (NO) and the activities of xanthine oxidase (XO), and superoxide dismutase (SOD) were used as both bio-indicators of oxidant status and determinant of antioxidant effect of CAPE. They were assessed by biochemical methods at 1st, 3rd, 7th, and 14th post-burn days. In conclusion, CAPE was shown to possess antioxidant activity by saving SOD activity, preventing XO activity and decreasing the levels of MDA, and NO. Our study showed that CAPE may be beneficial in burn injury.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Zonguldak Karaelmas University School of Medicine, Kozlu-Zonguldak 67600, Turkey. hosnuter@karaelmas.edu.tr
authors.forename: Mübin
authors.initials: M
authors.lastname: Hoşnuter
authors.forename: Ahmet
authors.initials: A
authors.lastname: Gürel
authors.forename: Orhan
authors.initials: O
authors.lastname: Babucçu
authors.forename: Ferah
authors.initials: F
authors.lastname: Armutcu
authors.forename: Eksal
authors.initials: E
authors.lastname: Kargi
authors.forename: Ahmet
authors.initials: A
authors.lastname: Işikdemir
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002056:Burns / Q000097:blood / Q000188:drug therapy*; D002109:Caffeic Acids / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009569:Nitric Oxide / Q000097:blood*; D018384:Oxidative Stress / Q000187:drug effects; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000097:blood; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002109:Caffeic Acids; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D008315:Malondialdehyde; D014969:Xanthine Oxidase; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol