Red blood cell ghosts and intact red blood cells as complementary models in photodynamic cell research.
Recent research on erythrocytes as model cells for photodynamic therapy showed differing behaviour of certain photosensitisers in erythrocytes compared to other cells. Differences of dye accumulation in the cell membrane were proposed to be the reason for the distinct photodynamic effects. Using pheophorbide a as an example, the combination of erythrocyte ghosts as models to follow the dye accumulation in the cell membrane and intact erythrocytes as model cells to show the photodynamic damage is provided. Evidence for the correctness of the combination of erythrocyte ghosts and intact erythrocytes as a functioning model system in photodynamic cell research is provided using the confocal laser scanning microscopy on intact, pheophorbide a loaded erythrocytes.
Kaestner
Lars
L
Institute for Molecular Cell Biology, Faculty of Medicine, Saarland University, Building 61, 66421 Homburg/Saar, Germany. biophysics@web.de
D002734:Chlorophyll / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D004912:Erythrocytes / Q000187:drug effects* / Q000528:radiation effects*; D006801:Humans; D018613:Microscopy, Confocal; D008954:Models, Biological; D010778:Photochemotherapy / Q000379:methods*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology / Q000627:therapeutic use; D012107:Research Design
D011838:Radiation-Sensitizing Agents; D002734:Chlorophyll; C032623:pheophorbide a