Comparative evaluation of D-glucosyl thiouronium, glucosylthio heterocycles, Daonil, and insulin as inhibitors for hepatic glycosidases.
Comparison of the in vivo and in vitro effects of S-(2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl)thiuronium bromide (1), 2-(2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosylthio-1,3,4-thiadiazolin-5-thione (2), and 2-(2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosylthio)-1,3-benzoxazole (3), as well as the antidiabetics Daonil and insulin on glycosidase enzymes has been investigated. Compound 1 inhibited both alpha- and beta-glucosidases, but the inhibition was more potent with the beta-enzyme. Compound 2 was found to be a weaker inhibitor of these enzymes, while compound 3 showed a slight apparent activation.
El din Awad
Olfat M
OM
Department of Biochemistry, Faculty of Science, Alexandria University, Alexandria 21321, Egypt.
Attia
Wafaa E
WE
El Ashry
El Sayed H
el SH
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D005905:Glyburide / Q000494:pharmacology*; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors* / Q000302:isolation & purification / Q000378:metabolism; D006571:Heterocyclic Compounds / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D051379:Mice; D015394:Molecular Structure; D013379:Substrate Specificity; D013863:Thioglucosides / Q000494:pharmacology*
D001786:Blood Glucose; D006571:Heterocyclic Compounds; D007328:Insulin; D013863:Thioglucosides; D006026:Glycoside Hydrolases; D005905:Glyburide