Repair of short oligodeoxyribonucleotides containing a stable adduct and an apurinic site by extracts of MCF-10A1 cells.
BACKGROUND
DNA with two sites of damage in close proximity might not be repaired as efficiently as DNA with a single damage site.


METHODS
To study this hypothesis, we utilized short oligodeoxyribonucleotides with a stable adduct 7 or 16 nucleotides (nt) downstream from an apurinic (AP) site. Repair by extracts of human breast epithelial MCF-10A1 cells was assayed by quantifying the incorporation of [alpha-32P]dTTP.


RESULTS
The level of repair of an oligodeoxyribonucleotide with an AP site 7 nt from a stable adduct was comparable to that of the oligodeoxyribonucleotide with only an AP site. A decrease in overall repair of oligodeoxyribonucleotides containing an AP site and a stable adduct was observed if these lesions were 16 nt apart compared to the presence of only an AP site.


CONCLUSIONS
The ability of human breast MCF-10A1 cells to repair DNA adducts and AP sites is affected by other near-by lesions.
Chapman
Kimberly A
KA
Eppley Institute for Research in Cancer and Allied Diseases and Department of Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska 68198-6805, USA.
Cavalieri
Ercole L
EL
Rogan
Eleanor G
EG
D001080:Apurinic Acid / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001940:Breast / Q000737:chemistry / Q000166:cytology; D002457:Cell Extracts / Q000494:pharmacology*; D002460:Cell Line; D018736:DNA Adducts / Q000235:genetics* / Q000378:metabolism; D004249:DNA Damage; D004260:DNA Repair / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000235:genetics; D003854:Deoxyribonucleotides / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics* / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000172:deficiency / Q000235:genetics; D051760:Xeroderma Pigmentosum Group A Protein
D002457:Cell Extracts; D018736:DNA Adducts; D004268:DNA-Binding Proteins; D003854:Deoxyribonucleotides; D009838:Oligodeoxyribonucleotides; D018808:Transcription Factor AP-1; C497314:XPA protein, human; D051760:Xeroderma Pigmentosum Group A Protein; D001080:Apurinic Acid