title: Evaluation of different methods to determine the loading of proteins in PLGA microspheres.
abstract: Various determination methods for protein encapsulation efficiency of PLGA microspheres were compared. Acetonitrile is recommended as an extraction solvent.
authors.affiliation: Department of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, PR China.
authors.forename: C H
authors.initials: CH
authors.lastname: Zheng
authors.forename: W Q
authors.initials: WQ
authors.lastname: Liang
authors.forename: H Y
authors.initials: HY
authors.lastname: Yu
authors.forename: H L
authors.initials: HL
authors.lastname: Chen
mesh_terms: D000097:Acetonitriles; D004339:Drug Compounding / Q000379:methods*; D019344:Lactic Acid; D008752:Methylene Chloride; D008863:Microspheres; D010316:Particle Size; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012995:Solubility; D012997:Solvents
chemical_list: D000097:Acetonitriles; D011108:Polymers; D011506:Proteins; D012997:Solvents; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; D008752:Methylene Chloride; C032159:acetonitrile