title: Salmonella enterica subsp. enterica serovar Enteritidis harbours ColE1, ColE2, and rolling-circle-like replicating plasmids.
abstract: Using DNA hybridization, at least three distinct groups of low molecular mass plasmids were identified in Salmonella enterica subsp. enterica serovar Enteritidis. After sequencing representative plasmids from each group, we concluded that they belonged to ColE1, ColE2, and rolling-circle-like replicating plasmids. Plasmid pK (4245 bp) is a representative of widely distributed ColE1 plasmids. Plasmid pP (4301 bp) is homologous to ColE2 plasmids and was present predominantly in single-stranded DNA form. The smallest plasmids pJ (2096 bp) and pB (1983 bp) were classified as rolling-circle-like replicating plasmids. Both encoded only a single protein essential for their own replication, and they must have existed in an unusual molecular structure, as (i) they were capable of hybridization without denaturation, (ii) their DNA could be linearized with S1 nuclease, and (iii) even after such treatment, the ability to hybridize without denaturation did not disappear.
authors.affiliation: Veterinary Research Institute, Hudcova 70, 621-32 Brno, Czech Republic.
authors.forename: Daniela
authors.initials: D
authors.lastname: Gregorova
authors.forename: Jitka
authors.initials: J
authors.lastname: Matiasovicova
authors.forename: Alena
authors.initials: A
authors.lastname: Sebkova
authors.forename: Marcela
authors.initials: M
authors.lastname: Faldynova
authors.forename: Ivan
authors.initials: I
authors.lastname: Rychlik
mesh_terms: D003086:Bacteriocin Plasmids; D004261:DNA Replication; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004277:DNA, Single-Stranded; D005111:Extrachromosomal Inheritance; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012477:Salmonella enteritidis / Q000235:genetics*; D017422:Sequence Analysis, DNA; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D015719:Single-Strand Specific DNA and RNA Endonucleases