Cyclophilin A is required for retinoic acid-induced neuronal differentiation in p19 cells.
Stable transfectants with expression of small interfering RNA for targeting cyclophilin A (CypA) in p19 cells lose their potential for retinoic acid (RA)-induced neuronal differentiation but not Me(2)SO-induced mesodermal differentiation. This difference suggests that CypA is specifically required for the RA-induced neuronal pathway. In addition to the loss of RA-induced RA receptor beta expression and retinoic acid response element (RARE)-binding activity, a dramatic reduction in RA-induced RARE-mediated luciferase activity in the CypA knockdown cell line suggests that CypA affects RARE-mediated regulation of gene expression. Silent mutation of target sequences confirms the specificity of RNA interference in p19 embryonal carcinoma cells. Collectively, our data reveal that a novel function of CypA is required in the processing of RA-induced neuronal differentiation in p19 embryonal carcinoma cells.
Song
Jun
J
Dental Research Institute, UCLA School of Dentistry, Los Angeles, California 90095, USA.
Lu
Ying-Chun
YC
Yokoyama
Kazunari
K
Rossi
John
J
Chiu
Robert
R
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002454:Cell Differentiation; D002460:Cell Line; D045744:Cell Line, Tumor; D021984:Cyclophilin A / Q000502:physiology*; D005786:Gene Expression Regulation; D008156:Luciferases / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010957:Plasmids / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D014212:Tretinoin / Q000378:metabolism*
D012333:RNA, Messenger; D034741:RNA, Small Interfering; D014212:Tretinoin; D008156:Luciferases; D021984:Cyclophilin A