Modification of membrane markers on THP-1 cells after ozone exposure in the presence or absence of fMLP.
The impacts of ozone and N-formyl-methionyl-leucyl-phenylalanine (fMLP) on the detection of membrane markers on non-differentiated THP-1 cells were evaluated for in vitro exposures. Several markers, specific for monocytes and macrophages, were identified on the THP-1 cells, allowing their use as a model for alveolar macrophages. Ozone exposure modified not only the detection of membrane markers, especially CD13 and CD14, monocyte and macrophage markers, but also the detection of the specific receptor for fMLP, formyl peptide receptor (FPR). Activation by fMLP also reduced the detection of the CD antigens, and a combined exposure to ozone and fMLP amplified this decrease, probably due to an additive effect of these chemicals. Overall, these results suggest important membrane rearrangements for short-term treatments to ozone and/or fMLP.
Klestadt
D
D
IUT de Thionville-Yutz, Laboratoire d'Immunologie-Microbiologie (ESE-CNRS, unité FRE2635), 1, Impasse A. Kastler, F-57970 Yutz, France. dklestadt@web.de
Laval-Gilly
P
P
Foucaud
L
L
Falla
J
J
D000393:Air Pollutants / Q000633:toxicity*; D015703:Antigens, CD / Q000032:analysis*; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D008856:Microscopy, Fluorescence; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D010126:Ozone / Q000633:toxicity*; D044042:Receptors, Formyl Peptide / Q000032:analysis
D000393:Air Pollutants; D015703:Antigens, CD; D044042:Receptors, Formyl Peptide; D009240:N-Formylmethionine Leucyl-Phenylalanine; D010126:Ozone