Fractionation of Trichoderma reesei cellulases by hydrophobic interaction chromatography on phenyl-sepharose.
Trichoderma reesei cellulase complex was fractionated using hydrophobic interaction chromatography with a phenyl-Sepharose column. Using a linear gradient of ammonium sulphate in the eluent buffer, a selective separation of endoglucanases was obtained at 15 degrees C with a four-fold increase in specific activity.
Tomaz
Cândida T
CT
Department of Chemistry, University of Beira Interior, 6201-001 Covilhã, Portugal. ctomaz@alpha2.ubi.pt
Queiroz
João A
JA
D000645:Ammonium Sulfate / Q000737:chemistry; D044602:Cellulases / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D005591:Chemical Fractionation / Q000379:methods*; D002847:Chromatography, Agarose / Q000379:methods*; D045424:Complex Mixtures / Q000737:chemistry* / Q000302:isolation & purification*; D004789:Enzyme Activation; D057927:Hydrophobic and Hydrophilic Interactions; D008970:Molecular Weight; D012685:Sepharose / Q000031:analogs & derivatives* / Q000737:chemistry*; D014242:Trichoderma / Q000201:enzymology*
D045424:Complex Mixtures; C020927:phenyl-sepharose; D012685:Sepharose; D044602:Cellulases; D000645:Ammonium Sulfate