Liquid storage, shipment, and cryopreservation of cord blood.
BACKGROUND
Cord blood banking requires methods for shipping and storage. This study examines the influence of shipping via overnight courier on postthaw viability of cord blood.


METHODS
Anticoagulated cord blood was divided with one sample diluted 1:1 using STM-sav (a storage solution) and the other undiluted. Units were shipped from Minneapolis to Memphis and returned, RBC-depleted, cryopreserved, stored for 14 days, and thawed. MNC counts, percent viable cells, quantity of CD34+ cells, and frequency of CFU-GM were measured. Temperature during shipment was continuously monitored.


RESULTS
Preliminary studies showed the packing and processing protocol influenced the temperatures experienced during shipping. Samples achieved temperatures below 10 degrees C within 4 to 8 hours with a few units dropping near or below 1 degrees C with cold ambient temperatures. The MNC recovery, CD34+45+ recovery, and frequency of CFU-GM for samples that were shipped were comparable to those observed using static liquid storage. The postthaw viable cell recovery was greatest for storage and shipping times of 24 hours and decreased when the storage and shipping times were longer.


CONCLUSIONS
Ambient conditions and the packing and processing protocol influence the temperature history of the sample. Samples stored beyond 24 hours in liquid storage and shipping exhibit a decreased postthaw recovery.
Hubel
Allison
A
Department of Mechanical Engineering, University of Minnesota, Minneapolis 55455, USA. hubel001@umn.edu
Carlquist
Dale
D
Clay
Mary
M
McCullough
Jeff
J
D018952:Antigens, CD34 / Q000032:analysis; D001773:Blood Cells / Q000166:cytology; D001793:Blood Preservation / Q000379:methods*; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods*; D005312:Fetal Blood; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology; D013696:Temperature; D014186:Transportation
D018952:Antigens, CD34