Enzymatic synthesis of monolaurin.
The aim of this study was to produce monolaurin utilizing a commercial immobilized lipase (Lipozyme IM-20; Novo Nordisk, Bagsvaerd, Denmark) through the direct esterification of lauric acid and glycerol in a solvent-free system. The influence of fatty acid/glycerol molar ratio, temperature, and Lipozyme (IM-20) concentration on the molar fraction of monolaurin were determined using an experimental design. The best conditions employed were 55 degrees C, lauric acid/glycerol molar ratio of 1.0, and 3.0% (w/w) enzyme concentration. The final product, obtained after 6 h of reaction, was 45.5% monolaurin, 26.8% dilaurin, 3.1% trilaurin, and 24.6% lauric acid. The reusability of the enzyme was also studied.
Pereira
Carla C B
CC
Escola de Química, Universidade Federal do Rio de Janeiro, Centro de Tecnologia, Bloco E, Lab I 221, Cidade Universitária, Brazil.
da Silva
Mônica A P
MA
Langone
Marta A P
MA
D001709:Biotechnology / Q000379:methods*; D002849:Chromatography, Gas; D004798:Enzymes / Q000737:chemistry; D004800:Enzymes, Immobilized; D004952:Esters; D005989:Glycerides / Q000096:biosynthesis* / Q000737:chemistry*; D005990:Glycerol / Q000737:chemistry; D007848:Laurates / Q000737:chemistry*; D007850:Lauric Acids / Q000737:chemistry; D008049:Lipase / Q000737:chemistry; D008962:Models, Theoretical; D050178:Monoglycerides; D013696:Temperature; D013997:Time Factors
D004798:Enzymes; D004800:Enzymes, Immobilized; D004952:Esters; D005989:Glycerides; D007848:Laurates; D007850:Lauric Acids; D050178:Monoglycerides; C030358:lauric acid; C020777:monolaurin; C093666:Lipozyme; D008049:Lipase; D005990:Glycerol