title: Dietary fat regulates mammary stearoyl coA desaturase expression and activity in lactating mice.
abstract: Mice in mid lactation (n=6 per group) were fed a control diet (1.19% fat), or diets containing safflower oil (25% w/w) or olive oil (25% w/w) for 7d. Mammary and liver stearoyl CoA desaturase (SCD) mRNA levels and mammary SCD activities were higher in lactating mice fed the control diet than in those fed the oil-supplemented diets. Further, mammary SCD mRNA was directly related to mammary SCD activity. Milk fat composition was influenced by dietary fat composition. The olive oil diet, high in 18:1, led to high levels of this fatty acid in milk and the safflower oil diet, high in 18:2, resulted in a milk fat with high levels of 18:2. These results show that there is regulation of SCD at the transcriptional level, associated with changes in enzyme activity and in milk fat composition.
authors.affiliation: AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand. kuljeet.singh@agresearch.co.nz
authors.forename: Kuljeet
authors.initials: K
authors.lastname: Singh
authors.forename: Darren G
authors.initials: DG
authors.lastname: Hartley
authors.forename: Thomas B
authors.initials: TB
authors.lastname: McFadden
authors.forename: Duncan D S
authors.initials: DD
authors.lastname: Mackenzie
mesh_terms: D000818:Animals; D004041:Dietary Fats / Q000494:pharmacology*; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D007774:Lactation; D008055:Lipids / Q000032:analysis; D008321:Mammary Glands, Animal / Q000201:enzymology*; D051379:Mice; D008892:Milk / Q000737:chemistry; D000069463:Olive Oil; D010938:Plant Oils / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D012450:Safflower Oil / Q000494:pharmacology; D013230:Stearoyl-CoA Desaturase / Q000235:genetics* / Q000378:metabolism
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D008055:Lipids; D000069463:Olive Oil; D010938:Plant Oils; D012333:RNA, Messenger; D012450:Safflower Oil; D013230:Stearoyl-CoA Desaturase