Fragmented DNA transport in dendrites of retinal neurons during apoptotic cell death.
Movement of fragmented DNA in dendrites of retinal neurons during the apoptotic cell death was investigated. The time-course of the movement of fragmented DNA in dendrites of retinal neurons undergoing apoptotic cell death induced by intravitreal N-methyl-d-aspartate (NMDA) injection were examined by in situ terminal dUTP-biotin nick end labeling of DNA fragments (TUNEL) method and fluorescence DNA detection technique by 4',6-diamidino-2-phenylindole (DAPI). The inhibitory effect of axoplasmic transport inhibitor, vincristine was also tested on the NMDA-induced fragmented DNA transport. The movement of fragmented DNA from apoptotic nuclei toward peripheral ends of the dendrites of the retinal neurons was clearly demonstrated. The transport of fragmented DNA, but not fragmentation per se, was completely inhibited by the co-administration of vincristine.
Hara
Akira
A
Department of Tumor Pathology, Gifu University School of Medicine, 40 tsukasa-machi, Gifu 500-8705, Japan. ahara@cc.gifu-u.ac.jp
Niwa
Masayuki
M
Kumada
Masako
M
Kitaori
Nami
N
Yamamoto
Tetsuya
T
Kozawa
Osamu
O
Mori
Hideki
H
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002452:Cell Count / Q000379:methods; D016923:Cell Death / Q000502:physiology; D004247:DNA / Q000378:metabolism*; D053938:DNA Fragmentation; D003712:Dendrites / Q000378:metabolism*; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D020287:In Situ Nick-End Labeling / Q000379:methods; D007211:Indoles / Q000494:pharmacology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016202:N-Methylaspartate / Q000494:pharmacology; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012160:Retina / Q000166:cytology*; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D013997:Time Factors; D014750:Vincristine / Q000494:pharmacology
D018690:Excitatory Amino Acid Agonists; D007211:Indoles; D015842:Serine Proteinase Inhibitors; C007293:DAPI; D014750:Vincristine; D016202:N-Methylaspartate; D004247:DNA