[Regulatory elements of copia retrotransposons, controlling the level of its expression in Drosophila melanogaster testes].
Expression of the lacZ reporter gene under the control of five deletion derivatives of the copia regulatory region including the 5' long terminal repeat (LTR) and the 5' untranslated region (UTR) was assayed in the testes of transgenic Drosophila melanogaster males (larvae and imago). The full-length copia regulatory region (LTR + UTR) ensured expression of the reporter gene in testes of both larvae and adult males. Deletion of UTR or 3' end of LTR increased lacZ expression in the testes, whereas deletion of the 5' end of LTR increased it. This indicated that a positive regulator of copia expression is at the 5' end of LTR and that negative regulators are at the 3' end of LTR and in UTR. The effects of the fragments of the copia regulatory region on reporter gene expression in the testes in vivo did not completely coincide with the effects observed earlier in cultured cells. We suggest that this difference is due to different regulation of expression of the fusion constructs integrated into chromatin as compared to their transient expression.
Morozova
T V
TV
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182 Russia. img@img.ras.ru
Tsybul'ko
E A
EA
Filatov
D A
DA
Pasiukova
E G
EG
D020121:5' Untranslated Regions; D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics*; D017930:Genes, Reporter; D008297:Male; D012091:Repetitive Sequences, Nucleic Acid; D018626:Retroelements; D013737:Testis / Q000378:metabolism*
D020121:5' Untranslated Regions; D018626:Retroelements