Distinct transcriptional programs in thymocytes responding to T cell receptor, Notch, and positive selection signals.
T cell antigen receptor (TCR) signaling is necessary but not sufficient to promote the positive selection of CD4+CD8+ thymocytes into CD4+ or CD8+ mature T cells. Notch signaling has also been implicated as a potential regulator of both CD4/CD8 T cell development and TCR signaling. However, the relationship between positive selection, TCR signaling, and Notch remains unclear. Here we use DNA microarray analysis to compare gene expression changes in CD4+CD8+ double-positive thymocytes undergoing positive selection, TCR stimulation, and Notch activation. We find that the genes induced during positive selection can be resolved into two distinct sets. One set, which we term "TCR-induced," is also induced by in vitro TCR stimulation and contains a large proportion of transcription factors. A second set, which we term "positive-selection-induced," is not induced by in vitro TCR simulation and contains a large proportion of genes involved in signal transduction pathways. Genes induced by Notch activity overlap substantially with genes induced during positive selection. We also find that Notch activity potentiates the effects of TCR stimulation on gene expression. These results help to identify TCR- and positive-selection-specific transcriptional events and help to clarify the relationship between positive selection and Notch.
Huang
Yina H
YH
Department of Molecular and Cell Biology, Division of Immunology and Cancer Research Laboratory, 475 LSA, University of California, Berkeley, CA 94720, USA.
Li
Dongling
D
Winoto
Astar
A
Robey
Ellen A
EA
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D051880:Receptors, Notch; D015398:Signal Transduction; D013950:Thymus Gland / Q000276:immunology*; D014158:Transcription, Genetic
D008565:Membrane Proteins; D011948:Receptors, Antigen, T-Cell; D051880:Receptors, Notch