title: Proteomic analysis of Arabidopsis glutathione S-transferases from benoxacor- and copper-treated seedlings.
abstract: Glutathione S-transferases (GSTs) are involved in many stress responses in plants, for example, participating in the detoxification of xenobiotics and limiting oxidative damage. Studies examining the regulation of this gene family in diverse plant species have focused primarily on RNA expression. A proteomics method was developed to identify GSTs expressed in Arabidopsis seedlings and to determine how the abundance of these proteins changed in response to copper, a promoter of oxidative stress, and benoxacor, a herbicide safener. Eight GSTs were identified in seedlings grown under control conditions, and only one, AtGSTU19, was induced by benoxacor. In contrast, four GSTs, AtGSTF2, AtGSTF6, AtGSTF7, and AtGSTU19, were significantly more abundant in copper-treated seedlings. The different responses to these treatments may reflect the potential for copper to affect many more aspects of plant growth and physiology compared with a herbicide safener. Differences between RNA and protein expression of GSTs indicate that both transcriptional and translational mechanisms are involved in regulation of GSTs under these conditions.
authors.affiliation: Department of Horticulture and Landscape Architecture and Chemistry, Purdue University, West Lafayette, Indiana 47907-2010, USA.
authors.forename: Aaron P
authors.initials: AP
authors.lastname: Smith
authors.forename: Ben P
authors.initials: BP
authors.lastname: DeRidder
authors.forename: Woei-Jiun
authors.initials: WJ
authors.lastname: Guo
authors.forename: Erin H
authors.initials: EH
authors.lastname: Seeley
authors.forename: Fred E
authors.initials: FE
authors.lastname: Regnier
authors.forename: Peter B
authors.initials: PB
authors.lastname: Goldsbrough
mesh_terms: D017360:Arabidopsis / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000737:chemistry* / Q000378:metabolism; D015151:Immunoblotting; D013058:Mass Spectrometry; D010078:Oxazines / Q000494:pharmacology*; D018384:Oxidative Stress; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D020543:Proteome; D040901:Proteomics / Q000379:methods; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology; D015262:Xenobiotics / Q000494:pharmacology
chemical_list: D010078:Oxazines; D010455:Peptides; D020543:Proteome; D015262:Xenobiotics; D012313:RNA; D003300:Copper; D005982:Glutathione Transferase; D014357:Trypsin; C085163:benoxacor