title: Synthesis and biological evaluation of 1,8-naphthyridin-4(1H)-on-3-carboxamide derivatives as new ligands of cannabinoid receptors.
abstract: Cannabinoid receptors have been studied extensively in view of their potential functional role in several physiological and pathological processes. For this reason, the search for new potent, selective ligands for subtype CB receptors, CB(1) and CB(2), is still of great importance, in order to investigate their role in various physiological functions. The present study describes the synthesis and the biological properties of a series of 1,8-naphthyridine derivatives, characterised by the presence of some important structural requirements exhibited by other classes of cannabinoid ligands, such as an aliphatic or aromatic carboxamide group in position 3, and an alkyl or arylalkyl substituent in position 1. These compounds were assayed for binding both to the brain and to peripheral cannabinoid receptors (CB(1) and CB(2)). The results obtained indicate that the naphthyridine derivatives examined possess a greater affinity for the CB(2) receptor than for the CB(1) receptor. In particular, derivatives 6a and 7a possess an appreciable affinity for the CB(2) receptor, with K(i) values of 5.5 and 8.0 nM respectively; also compounds 4a, 5a and 8a exhibit a good CB(2) affinity, with K(i) values in the range of 10-44 nM. Furthermore, compounds 3g-i and 18 revealed a good CB(2) selectivity, with a CB(1)/CB(2) ratio >20.
authors.affiliation: Dipartimento di Scienze Farmaceutiche, via Bonanno 6, 56126 Pisa, Italy. ferrarini@farm.unipi.it
authors.forename: Pier Luigi
authors.initials: PL
authors.lastname: Ferrarini
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Calderone
authors.forename: Tiziana
authors.initials: T
authors.lastname: Cavallini
authors.forename: Clementina
authors.initials: C
authors.lastname: Manera
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Saccomanni
authors.forename: Luca
authors.initials: L
authors.lastname: Pani
authors.forename: Stefania
authors.initials: S
authors.lastname: Ruiu
authors.forename: Gian Luigi
authors.initials: GL
authors.lastname: Gessa
mesh_terms: D000818:Animals; D004353:Drug Evaluation, Preclinical / Q000379:methods; D008024:Ligands; D008297:Male; D051379:Mice; D009287:Naphthyridines / Q000138:chemical synthesis* / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D043882:Receptors, Cannabinoid / Q000378:metabolism*
chemical_list: D008024:Ligands; D009287:Naphthyridines; D043882:Receptors, Cannabinoid