Antioxidant, a pro-oxidant and cytotoxic effects of Achyrocline satureioides extracts.
In this study we compared the antioxidant properties of five different extracts of different composition obtained from Achyrocline satureioides' inflorescences (Compositae), a widely used Brazilian folk medicinal herb. All of the extracts presented significant antioxidant potential identified by TRAP assay, which increased in the presence of human plasma. Characterization of the content of flavonoids in each extract showed that the FDP80 (ethanol 80%) and FFr (enriched flavonoid fraction) extracts contained a higher content of flavonoids. Cytotoxicity of the extracts as determined in Sertoli cell culture showed that FDP80 and FFr were highly toxic at most concentrations tested. The extracts induced a significant increase in lipid peroxidation levels in Sertoli cells. These results suggest that medicinal herb extracts that contain higher flavonoid concentrations and shows higher antioxidant protection in vitro might not always produce the greatest benefit.
Polydoro
M
M
Laboratório de Estresse Oxidativo, Departamento de Bioquímica, Instituto de Ciências Básicas de Saúde, Universidade Federal do Rio Grande do Sul. Rua Ramiro Barcelos, 2600 (anexo), Porto Alegre, RS 90035-003, Brazil.
de Souza
K C B
KC
Andrades
M E
ME
Da Silva
E G
EG
Bonatto
F
F
Heydrich
J
J
Dal-Pizzol
F
F
Schapoval
E E S
EE
Bassani
V L
VL
Moreira
J C F
JC
D036921:Achyrocline / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001938:Brazil; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000032:analysis; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000494:pharmacology; D012708:Sertoli Cells / Q000187:drug effects* / Q000473:pathology; D017392:Thiobarbituric Acid Reactive Substances
D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances