title: Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction-restriction fragment length polymorphism.
abstract: Angiostrongylus cantonensis, A. costaricensis, and A. vasorum are etiologic agents of human parasitic diseases. Their identification, at present, is only possible by examining the adult worm after a 40-day period following infection of vertebrate hosts with the third-stage larvae. In order to obtain a diagnostic tool to differentiate larvae and adult worm from the three referred species, polymerase chain reaction-restriction fragment length polymorphism was carried out. The rDNA second internal transcribed spacer (ITS2) and mtDNA cytochrome oxidase I regions were amplified, followed by digestion of fragments with the restriction enzymes RsaI, HapII, AluI, HaeIII, DdeI and ClaI. The enzymes RsaI and ClaI exhibited the most discriminating profiles for the differentiation of the regions COI of mtDNA and ITS2 of rDNA respectively. The methodology using such regions proved to be efficient for the specific differentiation of the three species of Angiostrongylus under study.
authors.affiliation: Laboratório de Helmintoses Intestinais, Centro de Pesquisas René Rachou-Fiocruz, Av. Augusto de Lima 1715, 30190-002 Belo Horizonte, MG, Brasil.
authors.forename: Roberta L
authors.initials: RL
authors.lastname: Caldeira
authors.forename: Omar S
authors.initials: OS
authors.lastname: Carvalho
authors.forename: Cristiane L
authors.initials: CL
authors.lastname: Mendonça
authors.forename: Carlos
authors.initials: C
authors.lastname: Graeff-Teixeira
authors.forename: Márcia C
authors.initials: MC
authors.lastname: Silva
authors.forename: Renata
authors.initials: R
authors.lastname: Ben
authors.forename: Rafael
authors.initials: R
authors.lastname: Maurer
authors.forename: Walter S
authors.initials: WS
authors.lastname: Lima
authors.forename: Henrique L
authors.initials: HL
authors.lastname: Lenzi
mesh_terms: D000801:Angiostrongylus / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D000818:Animals; D004262:DNA Restriction Enzymes / Q000032:analysis; D004272:DNA, Mitochondrial / Q000032:analysis; D005819:Genetic Markers; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000032:analysis; D013045:Species Specificity
chemical_list: D004272:DNA, Mitochondrial; D005819:Genetic Markers; D012335:RNA, Ribosomal; D004262:DNA Restriction Enzymes