Evaluation of the Velogene genomic assay for detection of vanA and vanB genes in vancomycin-resistant Enterococcus species.
The Velogene (VEL) genomic assay is a qualitative DNA probe for vanA and vanB in enterococci. 150 clinical isolates were tested with the VEL assay and characterized by pigment production, catalase levels, motility, growth in 6 micro g of vancomycin/ml, vancomycin and teicoplanin susceptibility, API 20S assay, and genotyping by multiplex PCR. The VEL assay identified all enterococcal strains with vanA and vanB genes.
Appleman
Maria D
MD
Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles County, California 90033, USA. mapplema@usc.edu
Citron
Diane M
DM
Kwok
Richard
R
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D019729:Carbon-Oxygen Ligases / Q000235:genetics*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D016983:Enterococcus / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D017334:Teicoplanin / Q000494:pharmacology; D014640:Vancomycin / Q000494:pharmacology; D020713:Vancomycin Resistance
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015342:DNA Probes; D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic; C071437:VanA ligase, Bacteria; C079913:VanB protein, Enterococcus; D017334:Teicoplanin; D014640:Vancomycin; D019729:Carbon-Oxygen Ligases