Improving the results of small-bore vascular graft testing in rabbits: a technical primer.
Appropriate models to evaluate the in vivo behavior of small-diameter grafts are varied. To evaluate the behavior of small-diameter, bovine-derived grafts in the arterial circulation, we chose the rabbit abdominal aorta model. In the development of our procedure, we evaluated several models published in the literature, with unsatisfactory results. The high incidence of postoperative mortality and morbidity led us to modify published methods to incorporate cautious surgical technique and mild systemic hypothermia with cross-clamp times shorter than 30 min, as well as perioperative administration of agents with metabolic, rheologic, and neuroprotective properties. These modifications enabled us to achieve 100% operative survival with a very low incidence of postoperative paralysis. The presented model will be used for further evaluation of small-diameter grafts in our laboratory.
Pekar
Filip
F
The International Heart Institute of Montana Foundation at St. Patrick Hospital and Health Sciences Center and the University of Montana, USA.
Grobe
Albert C
AC
Weber
Patricia A
PA
Cheung
David T
DT
Duran
Carlos M G
CM
D000818:Animals; D001012:Aorta, Abdominal / Q000637:transplantation*; D019917:Blood Vessel Prosthesis Implantation; D023421:Models, Animal; D011817:Rabbits; D014183:Transplantation, Heterologous