Nuclease Rsn from Rhizopus stolonifer: specificity and mode of action.
Nuclease Rsn from Rhizopus stolonifer catalyzes the hydrolysis of ss- and dsDNA in a ratio of approximately 2:1. Time course of 3' and 5' terminal analysis of the hydrolytic products of ss- and dsDNA showed that nuclease Rsn does not show any strict base preference and cleaves DNA in a non-specific manner. Moreover, separation of the hydrolytic products of ss- and dsDNA in the presence of Mg2+, Mn2+ or Co2+ showed the predominance of tetra-, tri-, and dinucleotides followed by mononucleotides, suggesting an endo mode of action.
Rangarajan
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Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India.
Shankar
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D000818:Animals; D002020:Buffaloes; D002417:Cattle; D003035:Cobalt / Q000737:chemistry / Q000378:metabolism; D004247:DNA / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism*; D006868:Hydrolysis; D007422:Intestines / Q000201:enzymology; D008099:Liver / Q000737:chemistry; D008274:Magnesium / Q000737:chemistry / Q000378:metabolism; D008345:Manganese / Q000737:chemistry / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D012233:Rhizopus / Q000201:enzymology*; D012910:Snake Venoms / Q000201:enzymology; D013154:Spleen / Q000201:enzymology; D013379:Substrate Specificity
D012910:Snake Venoms; D003035:Cobalt; D008345:Manganese; D004247:DNA; D004720:Endonucleases; D010727:Phosphoric Diester Hydrolases; D008274:Magnesium