Preparation and a time-resolved fluoroimmunoassay application of new europium fluorescent nanoparticles.
New silica-based europium fluorescent nanoparticles having surface amino groups were prepared by a covalent binding-copolymerization technique. In the nanoparticles, the fluorescent Eu3+ chelate molecules were covalently bound to silicon atoms to protect the nanoparticles from dye leaking in bio-applications. The amino groups on the surface of nanoparticles made the surface modification and bioconjugation of nanoparticles easier. The nanoparticles were characterized and developed as a new type of fluorescence probe for a highly sensitive time-resolved fluoroimmunoassay (TR-FIA) of human hepatitis B surface antigen (HBsAg).
Hai
Xiaodan
X
Department of Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012, PR China.
Tan
Mingqian
M
Wang
Guilan
G
Ye
Zhiqiang
Z
Yuan
Jingli
J
Matsumoto
Kazuko
K