Pharmacology of the agonist binding sites of rat neuronal nicotinic receptor subtypes expressed in HEK 293 cells.
The binding affinities of agonists at heteromeric nicotinic receptors composed of rat alpha2, alpha3 and alpha4 subunits in combination with beta2 or beta4 subunits were examined in stably transfected HEK 293 cells. In most cases, the affinities of agonists were higher at receptors composed of an alpha subunit in combination with the beta2 subunit than the beta4 subunit, and in some cases this difference was quite large (>250 times), suggesting the possibility of developing subtype-selective ligands and therapeutically useful drugs.
Xiao
Yingxian
Y
Department of Pharmacology, Georgetown University School of Medicine, Washington, DC 20057, USA.
Baydyuk
Maryna
M
Wang
Haizhu Pearl
HP
Davis
Heather E
HE
Kellar
Kenneth J
KJ
D000818:Animals; D001665:Binding Sites / Q000187:drug effects / Q000502:physiology; D001667:Binding, Competitive / Q000187:drug effects; D002460:Cell Line; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018722:Nicotinic Agonists / Q000494:pharmacology*; D021122:Protein Subunits / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011978:Receptors, Nicotinic / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D018722:Nicotinic Agonists; D021122:Protein Subunits; D011978:Receptors, Nicotinic