Forensic DNA-typing of dog hair: DNA-extraction and PCR amplification.
The forensic application of DNA-typing for the identification of dog hair provides objective evidence in the characterisation of traces found at crime scenes. During the past few years forensic dog identity testing has been improved considerably using multiplex PCR systems. However, DNA-typing from samples of one up to 10 dog hairs is often problematic in forensic science. A single dog hair contains very small quantities of DNA or the hair sample consists of hairs with roots of bad quality or even of broken hairshafts without roots. Here we describe an experimental study about dog hairs by means of a Ca(2+) improved DNA-extraction method, quantification and amplification.
Pfeiffer
I
I
Institute of Veterinary Medicine, Georg-August-University of Göttingen, Groner Landstrasse 2, Göttingen D-37073, Germany.
Völkel
I
I
Täubert
H
H
Brenig
B
B
D000818:Animals; D002021:Buffers; D002118:Calcium; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D004285:Dogs / Q000235:genetics*; D005554:Forensic Medicine / Q000379:methods*; D006197:Hair / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D020080:Tandem Repeat Sequences
D002021:Buffers; D004247:DNA; D002118:Calcium