title: Multiplex PCR for diagnosis of enteric infections associated with diarrheagenic Escherichia coli.
abstract: A multiplex PCR for detection of three categories of diarrheagenic Escherichia coli was developed. With this method, enterohemorrhagic E. coli, enteropathogenic E. coli, and enterotoxigenic E. coli were identified in fecal samples from patients with hemorrhagic colitis, watery diarrhea, or hemolytic-uremic syndrome and from food-borne outbreaks.
authors.affiliation: Programa de Microbiologia, Institode Ciencias Biomedicas, Facultad de Medicina, Universidad de Chile, Santiaago. rvidal@machi.med.uchile.cl
authors.forename: Roberto
authors.initials: R
authors.lastname: Vidal
authors.forename: Maricel
authors.initials: M
authors.lastname: Vidal
authors.forename: Rossana
authors.initials: R
authors.lastname: Lagos
authors.forename: Myron
authors.initials: M
authors.lastname: Levine
authors.forename: Valeria
authors.initials: V
authors.lastname: Prado
mesh_terms: D003092:Colitis / Q000175:diagnosis / Q000382:microbiology; D003967:Diarrhea / Q000175:diagnosis / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000382:microbiology; D019453:Escherichia coli O157 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D029968:Escherichia coli Proteins / Q000235:genetics; D005243:Feces / Q000382:microbiology; D005517:Foodborne Diseases / Q000175:diagnosis / Q000382:microbiology; D006463:Hemolytic-Uremic Syndrome / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D029968:Escherichia coli Proteins