title: Complement-independent Ab-induced peroxide lysis of platelets requires 12-lipoxygenase and a platelet NADPH oxidase pathway.
abstract: Antiplatelet GPIIIa49-66 Ab of HIV-related thrombocytopenic patients induces thrombocytopenia and platelet fragmentation by the generation of peroxide and other reactive oxygen species (ROS). Here we report the presence of a functional platelet NADPH oxidase pathway that requires activation by the platelet 12-lipoxygenase (12-LO) pathway to fragment platelets. A new Ab-mediated mechanism is described in which the platelet 12-LO product, 12(S)-HETE activates the NADPH oxidase pathway to generate ROS.
authors.affiliation: Department of Pediatrics, New York University School of Medicine, New York, New York 10016, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Nardi
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Feinmark
authors.forename: Liang
authors.initials: L
authors.lastname: Hu
authors.forename: Zongdong
authors.initials: Z
authors.lastname: Li
authors.forename: Simon
authors.initials: S
authors.lastname: Karpatkin
mesh_terms: D000906:Antibodies / Q000276:immunology*; D001092:Arachidonate 12-Lipoxygenase / Q000378:metabolism*; D001792:Blood Platelets / Q000276:immunology / Q000378:metabolism*; D003165:Complement System Proteins / Q000378:metabolism; D015658:HIV Infections / Q000378:metabolism; D015497:HIV-1; D006801:Humans; D009249:NADP / Q000378:metabolism; D019255:NADPH Oxidases / Q000378:metabolism*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism; D013921:Thrombocytopenia / Q000378:metabolism
chemical_list: D000906:Antibodies; D017382:Reactive Oxygen Species; D009249:NADP; D003165:Complement System Proteins; D001092:Arachidonate 12-Lipoxygenase; D019255:NADPH Oxidases