title: Evaluation of the BDProbeTec strand displacement amplification assay in comparison with the AMTD II direct test for rapid diagnosis of tuberculosis.
abstract: The BDProbeTec MTB assay for direct detection of Mycobacterium tuberculosis was evaluated in comparison with the AMTD-II assay on 94 samples from different patients with clinical suspicion of tuberculosis. Using a combination of culture on Lowenstein-Jensen medium (with or without preculture in BACTEC 9000) and clinical diagnosis as the standard, BDProbeTec MTB showed high sensitivity and specificity (96.1% and 100%, respectively), similar to AMTD-II (96.1% and 97.1%, respectively), with significantly higher sensitivity than the Ziehl-Neelsen stain for acid-fast bacilli (73%, p < 0.05).
authors.affiliation: Molecular Microbiology Unit, National Institute for Infectious Disease "Lazzaro Spallanzani"-I.R.C.C.S., Rome, Italy. mircomol@inmi.it
authors.forename: P
authors.initials: P
authors.lastname: Visca
authors.forename: P
authors.initials: P
authors.lastname: De Mori
authors.forename: A
authors.initials: A
authors.lastname: Festa
authors.forename: M L
authors.initials: ML
authors.lastname: Montrone
authors.forename: M
authors.initials: M
authors.lastname: Amicosante
authors.forename: L P
authors.initials: LP
authors.lastname: Pucillo
mesh_terms: D003470:Culture Media; D004251:DNA Transposable Elements / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D003470:Culture Media; D004251:DNA Transposable Elements; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D011933:Reagent Kits, Diagnostic