Oligonucleotide conjugates of Nile Red.
Oligonucleotides containing long chain amine on uridine-2'-carbamate have been covalently modified with Nile Red, a hydrophobic long wavelength fluorescent benzophenoxazine dye. The fluorescence of the dye is quenched in oligonucleotide conjugates. Thermal denaturation studies show considerable interactions of Nile Red with DNA-DNA duplexes.
Prokhorenko
Igor A
IA
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Dioubankova
Natalia N
NN
Korshun
Vladimir A
VA
D002219:Carbamates / Q000737:chemistry; D004247:DNA / Q000378:metabolism; D007700:Kinetics; D009841:Oligonucleotides / Q000737:chemistry*; D010078:Oxazines / Q000737:chemistry* / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013194:Staining and Labeling; D013696:Temperature
D002219:Carbamates; D009841:Oligonucleotides; D010078:Oxazines; D004247:DNA; C044808:nile red