title: Identification of enolase as a plasminogen-binding protein in excretory-secretory products of Fasciola hepatica.
abstract: We have followed a combined proteomic approach to identify proteins of Fasciola hepatica that could be involved in host-parasite interactions. Using two-dimensional gel electrophoresis, far Western immunoblot and mass spectrometry analyses, we have identified the enolase enzyme, present in the excretory/secretory materials of F. hepatica, as a human plasminogen-binding protein. This enzyme has an apparent molecular weight of 47 kDa with pI ranging from 6.2 to 7.2. These results suggest that enolase could act as a plasminogen receptor.
authors.affiliation: Departament de Bioquímica y Biología Molecular, Facultat de Ciències, Universitat de Valencia, C/Dr. Moliner, 50, 46100 Burjassot, Valencia, Spain.
authors.forename: Dolores
authors.initials: D
authors.lastname: Bernal
authors.forename: Jose Enrique
authors.initials: JE
authors.lastname: de la Rubia
authors.forename: Ana M
authors.initials: AM
authors.lastname: Carrasco-Abad
authors.forename: Rafael
authors.initials: R
authors.lastname: Toledo
authors.forename: Santiago
authors.initials: S
authors.lastname: Mas-Coma
authors.forename: Antonio
authors.initials: A
authors.lastname: Marcilla
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis; D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism*; D017124:Conserved Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D005210:Fasciola hepatica / Q000201:enzymology*; D015801:Helminth Proteins / Q000032:analysis* / Q000737:chemistry*; D006790:Host-Parasite Interactions; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008099:Liver / Q000469:parasitology; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010751:Phosphopyruvate Hydratase / Q000737:chemistry / Q000378:metabolism*; D010958:Plasminogen / Q000378:metabolism; D040901:Proteomics; D012756:Sheep / Q000469:parasitology
chemical_list: D000947:Antigens, Helminth; D002352:Carrier Proteins; D015801:Helminth Proteins; D010958:Plasminogen; D010751:Phosphopyruvate Hydratase