title: Biologically active antisense phosphorothioate oligodeoxyribonucleotides: synthesis, characterization, and studies of 3'-terminal phosphorothioate monoester analogues.
abstract: Multiple phosphorothioate oligonucleotides containing a 3'-terminal negative charge were synthesized and characterized. Influence of the added negative charge on activation of duplexes by RNase H was investigated. No additional help in recruitment of RNase H was observed.
authors.affiliation: Isis Pharmaceuticals, Carlsbad, California 92008, USA. vravikumar@isisph.com
authors.forename: Vasulinga T
authors.initials: VT
authors.lastname: Ravikumar
authors.forename: Walt F
authors.initials: WF
authors.lastname: Lima
authors.forename: Kent
authors.initials: K
authors.lastname: Van Sooy
authors.forename: Brett
authors.initials: B
authors.lastname: Turney
mesh_terms: D020319:Oligodeoxyribonucleotides, Antisense / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D020319:Oligodeoxyribonucleotides, Antisense