The biological activity in mammals and insects of the nucleosidic fraction from the spider Parawixia bistriata.
Previous work has shown that the crude venom of Parawixia bistriata induces convulsive seizures in rats after intracerebroventricular injection. In this work, the isolation of a bioactive fraction with ultraviolet absorption characteristics of nucleic acid and trace protein or amino acid content is described. NMR analysis demonstrated that the major component of the active fraction is the nucleoside inosine. An analogue of this component (inosine 5'-monophosphate) induced a delayed paralysis effect in termites.
Rodrigues
Marcelo Cairrão Araujo
MC
Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto da, Universidade de São Paulo, Av. Bandeirantes 3900, Ribeirão Preto, 14049-901 São Paulo, Brazil.
Guizzo
Renato
R
Gobbo-Neto
Leonardo
L
Ward
Richard John
RJ
Lopes
Norberto Peporine
NP
dos Santos
Wagner Ferreira
WF
D000818:Animals; D001681:Biological Assay; D007288:Inosine / Q000737:chemistry; D007291:Inosine Monophosphate / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity*; D020049:Isoptera / Q000187:drug effects* / Q000502:physiology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013111:Spider Venoms / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity*; D013112:Spiders / Q000737:chemistry*
D013111:Spider Venoms; D007291:Inosine Monophosphate; D007288:Inosine