Peripheral blood circulating immature cell counts predict CD34+ cell yields in G-CSF-induced PBPC mobilization in healthy donors.
BACKGROUND
It has been previously reported that the number of circulating immature cells (CIC) in peripheral blood (PB) estimates the number of CD34+ cells collected in G-CSF plus chemotherapy-induced PBPC mobilization. The correlation of CIC counts in PB with CD34+ cell yield and its usefulness was evaluated in G-CSF-induced PBPC mobilization for healthy donors.


METHODS
CIC counts in PB and CD34+ cell counts in the apheresis product from 122 collections were assessed, and the relationship between these two variables was evaluated with the Pearson rank correlation analysis, the chi-squared test, and the U-test.


RESULTS
CIC counts were correlated weakly with the number of CD34+ cells per L of blood processed in the apheresis product (Pearson rank correlation analysis; r=0.357, p<0.0001). When a level of 1.7 x 10(9) CICs per L was selected as a cutoff value, the sensitivity and specificity for collecting more than 20 x 10(6) CD34+ cells per L of blood processed were 63.6 and 77.5 percent, respectively.


CONCLUSIONS
The present study suggests that the number of CICs in PB may estimate the number of CD34+ cells collected. The data indicate that CIC counts above 1.7 x 10(9) per L can be used as a good predictor for PBPC collections containing more than 20 x 10(6) CD34+ cells per L of blood processed in a single apheresis procedure.
Kozuka
Teruhiko
T
Second Department of Internal Medicine, Division of Blood Transfusion, Okayama University Medical School, Japan. c-tkozuka@eph.pref.ehime.jp
Ikeda
Kazuma
K
Teshima
Takanori
T
Yoshida
Chikamasa
C
Shinagawa
Katsuji
K
Kojima
Kensuke
K
Matsuo
Keitaro
K
Bessho
Akihiro
A
Sunami
Kazutaka
K
Hiramatsu
Yasushi
Y
Maeda
Yoshinobu
Y
Noguchi
Toshio
T
Yamamoto
Kazuhiko
K
Fujii
Nobuharu
N
Imai
Toshi
T
Kusumoto
Kinuyo Kaneda
KK
Masuda
Kozo
K
Takenaka
Katsuto
K
Ishimaru
Fumihiko
F
Niiya
Kenji
K
Koide
Norio
N
Tanimoto
Mitsune
M
Harada
Mine
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis*; D001772:Blood Cell Count; D001773:Blood Cells; D001781:Blood Component Removal; D001782:Blood Donors; D004305:Dose-Response Relationship, Drug; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage* / Q000494:pharmacology; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012372:ROC Curve
D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor