Activity of (-)-epigallocatechin 3-O-gallate against oxidative stress in rats with adenine-induced renal failure.
Methylguanidine (MG) is widely recognized as a strong uremic toxin. The hydroxyl radical (*OH) specifically plays an important role in the pathway of MG production from creatinine (Cr). In this study, we investigated whether oral administration of (-)-epigallocatechin 3-O-gallate (EGCg) suppresses MG production in rats with chronic renal failure after intraperitoneal Cr injection. MG production from Cr was significantly increased in rats with adenine-induced renal failure, which was more vulnerable to oxidative stress, compared with that in normal rats. However, oral administration of EGCg 30 min before and after Cr injection effectively inhibited MG production. Our findings suggest that EGCg, an excellent antioxidant from green tea, exerts protective activity in rats with chronic renal failure, resulting in suppression of Cr oxidation influenced by *OH.
Nakagawa
Takako
T
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan.
Yokozawa
Takako
T
Sano
Mitsuyo
M
Takeuchi
Shigeya
S
Kim
Mujo
M
Minamoto
Shinsuke
S
D000225:Adenine; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001806:Blood Urea Nitrogen; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D003404:Creatinine / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism; D017665:Hydroxyl Radical / Q000737:chemistry; D007676:Kidney Failure, Chronic / Q000139:chemically induced* / Q000378:metabolism*; D008297:Male; D008760:Methylguanidine / Q000032:analysis / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D017665:Hydroxyl Radical; D008760:Methylguanidine; D002392:Catechin; D003404:Creatinine; C045651:epigallocatechin gallate; D000225:Adenine