Enhanced production of nikkomycin X by over-expression of SanO, a non-ribosomal peptide synthetase in Streptomyces ansochromogenes.
A recombinant strain was constructed by introducing pWO6 which contains the sanO gene encoding a non-ribosomal peptide synthetase (NRPS) into Streptomyces ansochromogenes 7100 (wild-type strain). The bioassay of nikkomycin indicated that the anti-fungal activity was enhanced in the recombinant strain in comparison with the wild-type strain. HPLC analysis revealed that 1200 mg nikkomycin X l(-1) was produced in the recombinant strain after growth for 6 d, which was about twice that of the wild-type strain. RT-PCR also indicated that higher transcription of the sanO gene was observed in the recombinant strain in contrast with wild-type strain.
Wang
Guojun
G
Department of Molecular Microbiology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
Tan
Huarong
H
D000617:Aminoglycosides / Q000096:biosynthesis* / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D024861:Genetic Enhancement / Q000379:methods*; D010453:Peptide Synthases / Q000096:biosynthesis* / Q000235:genetics*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics*
D000617:Aminoglycosides; D011994:Recombinant Proteins; C049372:nikkomycin X; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase