title: In vitro evaluation of cyanovirin-N antiviral activity, by use of lentiviral vectors pseudotyped with filovirus envelope glycoproteins.
abstract: Cyanovirin-N (CV-N) has been shown to inhibit Ebola Zaire virus (EboZV) infection, both in vitro and in vivo, through its ability to bind to oligomannoses-8/9 on the EboZV surface glycoprotein (GP). Here, we report the in vitro potency of CV-N to inhibit EboZV GP- and Marburg virus GP-pseudotyped viruses (EC50 approximately 40-60 nmol/L and approximately 6-25 nmol/L, respectively) from mediating gene transduction into HeLa cells. In addition, we provide evidence that CV-N can effectively inhibit DC-SIGN-mediated EboZV infection. Our data emphasize both the utility of GP-pseudotyped vectors in the assessment of compounds that affect cell entry by filovirus and the use of CV-N as a reagent for the probing of carbohydrate-dependent interactions at viral entry.
authors.affiliation: Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. lbarrientos1@cdc.gov
authors.forename: Laura G
authors.initials: LG
authors.lastname: Barrientos
authors.forename: Fatima
authors.initials: F
authors.lastname: Lasala
authors.forename: Joaquin R
authors.initials: JR
authors.lastname: Otero
authors.forename: Anthony
authors.initials: A
authors.lastname: Sanchez
authors.forename: Rafael
authors.initials: R
authors.lastname: Delgado
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000494:pharmacology*; D029043:Ebolavirus / Q000187:drug effects* / Q000378:metabolism; D006023:Glycoproteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D006367:HeLa Cells; D019142:Hemorrhagic Fever, Ebola / Q000517:prevention & control* / Q000821:virology; D006801:Humans; D019169:Jurkat Cells; D016086:Lentivirus / Q000378:metabolism; D008379:Marburg Virus Disease / Q000188:drug therapy* / Q000821:virology; D029024:Marburgvirus / Q000187:drug effects* / Q000378:metabolism; D014161:Transduction, Genetic; D014764:Viral Proteins; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D006023:Glycoproteins; D014764:Viral Proteins; C110613:secreted glycoprotein, Ebola virus; C107733:cyanovirin N