title: Peripheral blood circulating immature cell counts predict CD34+ cell yields in G-CSF-induced PBPC mobilization in healthy donors.
abstract: BACKGROUND
  It has been previously reported that the number of circulating immature cells (CIC) in peripheral blood (PB) estimates the number of CD34+ cells collected in G-CSF plus chemotherapy-induced PBPC mobilization. The correlation of CIC counts in PB with CD34+ cell yield and its usefulness was evaluated in G-CSF-induced PBPC mobilization for healthy donors.
  
  
  METHODS
  CIC counts in PB and CD34+ cell counts in the apheresis product from 122 collections were assessed, and the relationship between these two variables was evaluated with the Pearson rank correlation analysis, the chi-squared test, and the U-test.
  
  
  RESULTS
  CIC counts were correlated weakly with the number of CD34+ cells per L of blood processed in the apheresis product (Pearson rank correlation analysis; r=0.357, p<0.0001). When a level of 1.7 x 10(9) CICs per L was selected as a cutoff value, the sensitivity and specificity for collecting more than 20 x 10(6) CD34+ cells per L of blood processed were 63.6 and 77.5 percent, respectively.
  
  
  CONCLUSIONS
  The present study suggests that the number of CICs in PB may estimate the number of CD34+ cells collected. The data indicate that CIC counts above 1.7 x 10(9) per L can be used as a good predictor for PBPC collections containing more than 20 x 10(6) CD34+ cells per L of blood processed in a single apheresis procedure.
authors.affiliation: Second Department of Internal Medicine, Division of Blood Transfusion, Okayama University Medical School, Japan. c-tkozuka@eph.pref.ehime.jp
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Kozuka
authors.forename: Kazuma
authors.initials: K
authors.lastname: Ikeda
authors.forename: Takanori
authors.initials: T
authors.lastname: Teshima
authors.forename: Chikamasa
authors.initials: C
authors.lastname: Yoshida
authors.forename: Katsuji
authors.initials: K
authors.lastname: Shinagawa
authors.forename: Kensuke
authors.initials: K
authors.lastname: Kojima
authors.forename: Keitaro
authors.initials: K
authors.lastname: Matsuo
authors.forename: Akihiro
authors.initials: A
authors.lastname: Bessho
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Sunami
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hiramatsu
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Maeda
authors.forename: Toshio
authors.initials: T
authors.lastname: Noguchi
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Nobuharu
authors.initials: N
authors.lastname: Fujii
authors.forename: Toshi
authors.initials: T
authors.lastname: Imai
authors.forename: Kinuyo Kaneda
authors.initials: KK
authors.lastname: Kusumoto
authors.forename: Kozo
authors.initials: K
authors.lastname: Masuda
authors.forename: Katsuto
authors.initials: K
authors.lastname: Takenaka
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Ishimaru
authors.forename: Kenji
authors.initials: K
authors.lastname: Niiya
authors.forename: Norio
authors.initials: N
authors.lastname: Koide
authors.forename: Mitsune
authors.initials: M
authors.lastname: Tanimoto
authors.forename: Mine
authors.initials: M
authors.lastname: Harada
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis*; D001772:Blood Cell Count; D001773:Blood Cells; D001781:Blood Component Removal; D001782:Blood Donors; D004305:Dose-Response Relationship, Drug; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage* / Q000494:pharmacology; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012372:ROC Curve
chemical_list: D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor