Structural studies of water-soluble polysaccharides of an edible mushroom, Termitomyces eurhizus. A reinvestigation.
The structure of two polysaccharides isolated from the hot aqueous extract of fruiting bodies of the mushroom, Termitomyces eurhizus, have been reinvestigated. These consist of two homogeneous fractions PS-I and PS-II. PS-I contains only D-glucose as the monosaccharide constituent. From methylation analysis and periodate oxidation studies, followed by GLC-MS analysis the linkages, the sugar units in PS-I were identified as (1-->3)-D-Glcp and (1-->6)-D-Glcp. PS-II contains D-glucose, and the mode of linkage of d-glucose was identified as (1-->6)-D-Glcp. Finally, the following possible structures of the polysaccharides were assigned using 1H, 2D-COSY, TOCSY, NOESY and 13C NMR spectral analysis: [carbohydrate structure: see text].
Mondal
Soumitra
S
Department of Chemistry and Chemical Technology, Vidyasagar University, West Midnapore 721102, West Bengal, India.
Chakraborty
Indranil
I
Pramanik
Malay
M
Rout
Dilip
D
Islam
Syed S
SS
D000363:Agaricales; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry; D002849:Chromatography, Gas; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry*; D011522:Protons; D013053:Spectrophotometry; D013997:Time Factors; D014867:Water / Q000737:chemistry*
D002241:Carbohydrates; D009005:Monosaccharides; D011134:Polysaccharides; D011522:Protons; D014867:Water; D010100:Oxygen