High-performance liquid chromatographic determination of primary amines in aqueous solutions after extraction and derivatization with 2,2-diphenyl-1-oxa-3-oxonia-2-boratanaphthalene (DOOB).
Aqueous solutions of tryptamine, tyramine and ethanolamine are extracted and derivatizated to azomethine chelates using a solution of DOOB in diethyl ether. The extraction takes about 2 h. Diisopropyl ether and toluol have also been tested as extractants. The separation of the azomethine chelates is realized by a gradient program on a Si 60 column using a mixture of n-heptane/tert-butyl methyl ether. UV detection is carried out at 280 nm. The concentration of these three amines has been determined in a Chianti wine sample.
Sanchez-Rodas
D
D
Fachbereich 8, Analytische Chemie I, Universität-GH-Siegen, Adolf Reichwein-Strasse 2, D-57068, Siegen, Germany.
Hohaus
E
E
Wenclawiak
B
B