Lymph sampling and lymphangiography via EUS-guided transesophageal thoracic duct puncture in a swine model.
BACKGROUND
The thoracic duct plays an important role in fluid balance, lipid absorption, and transport of immunologically active cells. The thoracic duct is difficult to access but is readily identified by EUS. The present study assessed the feasibility of EUS identification and EUS-guided puncture of the thoracic duct in a swine model.


METHODS
Transesophageal EUS-guided thoracic duct puncture was attempted in 3 animals under fluoroscopy. In two animals, the thoracic duct was identified by cutdown in the neck; in the third animal, EUS-guided puncture was performed under fluoroscopy alone. In one animal, the thoracic duct was opacified by injecting contrast medium. In the second animal, thoracic duct was injected with patent blue and contrast medium.


METHODS
Thoracic duct was identified and punctured successfully in all 3 animals. In the first animal, contrast medium containing lymph was aspirated. In the second animal, lymph containing patent blue was aspirated, and, in the third animal, successful puncture was confirmed by obtaining a lymphangiogram.


CONCLUSIONS
This pilot study in an animal model demonstrates that transesophageal EUS-guided puncture of the thoracic duct is feasible and that the technique can be used for lymph sampling and/or lymphangiography.
Parasher
Vinod K
VK
Helen F. Graham Cancer Center, Christiana Care Hospital, Newark, Delaware 19958, USA.
Hernandez
Lyndon V
LV
Leveen
Robert F
RF
Mladinich
Christopher R J
CR
Nonabur
Venkatesh
V
Bhutani
Manoop S
MS
D000818:Animals; D019160:Endosonography; D005240:Feasibility Studies; D008220:Lymphography / Q000379:methods*; D023421:Models, Animal; D011677:Punctures; D013552:Swine; D013897:Thoracic Duct / Q000000981:diagnostic imaging* / Q000601:surgery*