In vitro effects of recombinant human growth hormone on growth of human gastric cancer cell line BGC823 cells.
OBJECTIVE
To study the effects of recombinant human growth hormone (rhGH) on growth of human gastric cancer cell line in vitro.


METHODS
Experiment was divided into control group, rhGH group, oxaliplatin (L-OHP) group and rhGH+L-OHP group. Cell inhibitory rate, cell cycle, cell proliferation index (PI) and DNA inhibitory rate of human gastric cancer line BGC823, at different concentrations of rhGH treatment were studied by cell culture, MTT assay and flow cytometry.


RESULTS
The distinctly accelerated effects of rhGH on multiplication of BGC823 cell line were not found in vitro. There was no statistical significance between rhGH group and control group, or between rhGH+L-OHP group and L-OHP group (P>0.05). The cell growth curve did not rise. Cell inhibitory rate and cells arrested in G(0)-G(1) phase were obviously increased. Meanwhile, cells in S phase and PI were distinctly decreased and DNA inhibitory rate was obviously increased in rhGH+L-OHP group in comparison with control group and rhGH group, respectively (P<0.01). Cell inhibitory rate showed an increasing trend and PI showed a decreasing trend in rhGH+L-OHP group compared with L-OHP group.


CONCLUSIONS
In vitro rhGH does not accelerate the multiplication of human gastric cancer cells. It may increase the therapeutic efficacy when it is used in combination with anticancer drugs.
Chen
Jia-Yong
JY
Department of General Surgery of the Second Affiliated Hospital, Kunming Medical College, Kunming 650101, Yunnan Province, China. chenjiayong776@.hotmail.com
Liang
Dao-Ming
DM
Gan
Ping
P
Zhang
Yi
Y
Lin
Jie
J
D000970:Antineoplastic Agents / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor / Q000166:cytology / Q000187:drug effects; D004396:Coloring Agents; D004247:DNA / Q000096:biosynthesis; D016193:G1 Phase / Q000187:drug effects; D013006:Growth Hormone / Q000494:pharmacology*; D019382:Human Growth Hormone / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D009944:Organoplatinum Compounds / Q000494:pharmacology; D000077150:Oxaliplatin; D016192:Resting Phase, Cell Cycle / Q000187:drug effects; D013274:Stomach Neoplasms; D013778:Tetrazolium Salts; D013844:Thiazoles
D000970:Antineoplastic Agents; D004396:Coloring Agents; D009944:Organoplatinum Compounds; D013778:Tetrazolium Salts; D013844:Thiazoles; D000077150:Oxaliplatin; D019382:Human Growth Hormone; D013006:Growth Hormone; D004247:DNA; C022616:thiazolyl blue