[Biocompatibility of p(3HB-co-3HH) and marrow mesenchymal stem cells].
OBJECTIVE
To investigate the biocompatibility of p(3HB-co-3HH) and marrow mesenchymal stell cells (MSCs).


METHODS
MSCs were inoculated to p(3HB-co-3HH), and then cultured for 2-4 weeks in vitro and embedded for 2 weeks in vivo. The growth, proliferation, morphology and phenotype properties of MSCs were observed by use of phase contrast microscope, electron microscope, HE staining and staining of type I collagen.


RESULTS
p(3HB-co-3HH) had good compatibility. The inoculated MSCs could be well-distributed, attached well and obtain the phenotype of MSCs in p(3HB-co-3HH). After osteogenic inducer were added, MSCs differentiated to osteoblasts and secreted matrix. Type I collagen was stained positively by immunohistochemical techenique.


CONCLUSIONS
The above results demonstrate that there is satisfactory biocompatibility between p(3HB-co-3HH) and MSCs.
Zhao
Yu
Y
Department of Plastic Surgery, First Affiliated Hospital of Anhui University of Medical Science, Hefei, Anhui, P. R. China 230022.
Hu
Ping
P
Lu
Ying-ling
YL
D020155:3-Hydroxybutyric Acid; D000818:Animals; D001672:Biocompatible Materials; D001854:Bone Marrow Cells / Q000166:cytology*; D002208:Caproates; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008819:Mice, Nude; D023822:Tissue Engineering; D000094122:Polyhydroxybutyrates
D020155:3-Hydroxybutyric Acid; D001672:Biocompatible Materials; D002208:Caproates; D024042:Collagen Type I; C115940:poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates