Proteomics for nasal secretion analysis.
Since the completion of the human genome, the interest of the scientific community has evolved toward understanding the human proteome. The genomic and proteomic data will facilitate our understanding of the functions of proteins in diseases and the discovery of novel drug target proteins and biomarkers of diseases. Highly sensitive analytic techniques are necessary to study the complexity of biologic samples. The key to any proteomics experiment is to reduce the complexity of the sample before mass spectrometry (MS) analysis. Numerous separation techniques have been used, including one- and two-dimensional gel electrophoresis, chromatography, and affinity techniques. MS has become a powerful method for analyzing biologic samples. This review does not cover all aspects of proteomics, but is intended to give an introductory explanation of the technology using the example of the proteomics of nasal secretions.
Casado
Begoña
B
Department of Medicine, Division of Rheumatology, Immunology and Allergy, Georgetown University, Lower Level Gorman Building, Room GL008, 3800 Reservoir Road, NW, Washington, DC 20057, USA. bc48@georgetown.edu
D015415:Biomarkers / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D013058:Mass Spectrometry; D009297:Nasal Mucosa / Q000378:metabolism*; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification; D040901:Proteomics
D015415:Biomarkers; D011506:Proteins