Effects of sugar inhibition on cellulases and beta-glucosidase during enzymatic hydrolysis of softwood substrates.
A quantitative approach was taken to determine the inhibition effects of glucose and other sugar monomers during cellulase and beta-Glucosidase hydrolysis of two types of cellulosic material: Avicel and acetic acid-pretreated softwood. The increased glucose content in the hydrolysate resulted in a dramatic increase in the degrees of inhibition on both beta-Glucosidase and cellulase activities. Supplementation of mannose, xylose, and galactose during cellobiose hydrolysis did not show any inhibitory effects on beta-Glucosidase activity. However, these sugars were shown to have significant inhibitory effects on cellulase activity during cellulose hydrolysis. Our study suggests that high-substrate consistency hydrolysis with supplementation of hemicellulose is likely to be a practical solution to minimizing end-product inhibition effects while producing hydrolysate with high glucose concentration.
Xiao
Zhizhuang
Z
Forest Products Biotechnology, University of British Columbia, 4033-2424 Main Mall, Vancouver BC, V6T 1Z4 Canada.
Zhang
Xiao
X
Gregg
David J
DJ
Saddler
John N
JN
D019342:Acetic Acid / Q000737:chemistry; D002480:Cellulase / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry; D008358:Mannose / Q000737:chemistry; D013997:Time Factors; D014462:Ultrafiltration; D014867:Water / Q000737:chemistry; D014934:Wood; D001617:beta-Glucosidase / Q000737:chemistry* / Q000378:metabolism
D014867:Water; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; D001617:beta-Glucosidase; D002480:Cellulase; D005947:Glucose; D008358:Mannose; D019342:Acetic Acid; D005690:Galactose