Cell immersion and cell dipping in microfluidic devices.
Combining deflective dielectrophoretic barriers with controlled pressure driven liquid flows in microfluidic devices allows accurate handling of particles such as biological cells in suspensions. Working towards cell-based lab-on-a-chip applications, a platform permitting rapid testing of devices having different dielectrophoretic and fluidic subunits was developed. The performance of such a system is shown in the cases of (A) flooding a small number of immobilised cells with a dye and (B) transient buffer swapping of a large number of cells in flow. The transition times for moving cells from one reagent to the other are below 0.5 s in the case of flow-through cell dipping.
Seger
Urban
U
Swiss Federal Institute of Technology, EPFL, STI-LMIS, CH-1015 Lausanne, Switzerland. urban.seger@epfl.ch
Gawad
Shady
S
Johann
Robert
R
Bertsch
Arnaud
A
Renaud
Philippe
P
D001709:Biotechnology / Q000295:instrumentation / Q000379:methods; D002021:Buffers; D018914:Cells, Immobilized; D004396:Coloring Agents; D003584:Cytological Techniques / Q000295:instrumentation / Q000379:methods*; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D004912:Erythrocytes; D006801:Humans; D007700:Kinetics; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods*
D002021:Buffers; D004396:Coloring Agents