CITED1 protein expression suggests Papillary Thyroid Carcinoma in high throughput tissue microarray-based study.
Molecular markers of papillary thyroid carcinoma (PTC) are relatively unknown. Recently, the CITED1 gene was reported to be greatly upregulated in PTC relative to normal thyroid. The CITED1 protein, a 27-kd transcriptional transactivator nuclear protein is expressed in PTC, melanocytes, breast epithelial cells, and several embryonic tissues. However, its expression in other thyroid masses and non-thyroid tumors is not known. We evaluated CITED1 protein expression in tissue microarrays comprising various thyroid and nonthyroid tissues by immunohistochemistry using a polyclonal anti-CITED1 antibody. CITED1 expression was seen in 63 of 68 PTC (93%), 3 of 12 follicular carcinomas (25%), 2 of 7 Hürthle cell carcinomas (28%), 2 of 21 adenomas (10%), 2 of 6 follicular neoplasms of undetermined malignant behavior (33%), and 2 of 24 nodular goiters (8%). Normal thyroids (n = 27), thyrotoxic hyperplasias (n = 14), and anaplastic thyroid carcinomas (n = 5) did not express CITED1. Among nonthyroid tumors, 6 of 23 melanomas (26%), 11 of 65 prostatic carcinomas (17%), 3 of 25 glioblastomas (12%), 4 of 67 breast carcinomas (6%), 1 of 49 lymphomas (2%), 1 of 65 lung carcinomas (2%), 1 of 68 colon carcinomas (2%), and none of 49 ovarian carcinomas (0%) expressed CITED1. The accuracy of CITED1 in differentiating PTC from benign thyroid nodules, other thyroid carcinomas, and nonthyroid carcinomas was 93%, 89%, and 94%, respectively. CITED1 is preferentially expressed in PTC and may be used as a diagnostic marker of it.
Prasad
Manju L
ML
Department of Pathology, Ohio State University, Columbus, Ohio 43210, USA. Prasad-l@medctr.osu.edu
Pellegata
Natalia S
NS
Kloos
Richard T
RT
Barbacioru
Catalin
C
Huang
Ying
Y
de la Chapelle
Albert
A
D018263:Adenocarcinoma, Follicular / Q000378:metabolism; D000236:Adenoma / Q000378:metabolism; D018249:Adenoma, Oxyphilic / Q000378:metabolism; D051017:Apoptosis Regulatory Proteins; D002277:Carcinoma / Q000175:diagnosis / Q000378:metabolism; D002291:Carcinoma, Papillary / Q000175:diagnosis* / Q000378:metabolism*; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry; D009687:Nuclear Proteins; D020411:Oligonucleotide Array Sequence Analysis; D012680:Sensitivity and Specificity; D013964:Thyroid Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D016606:Thyroid Nodule / Q000175:diagnosis / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors
D051017:Apoptosis Regulatory Proteins; C512688:CITED1 protein, human; D009687:Nuclear Proteins; D015534:Trans-Activators; D014157:Transcription Factors