Heat-induced production of human growth hormone by high cell density cultivation of recombinant Escherichia coli.
The temperature-induced, over-expression of the human growth hormone gene in a recombinant E. coli during high cell density cultivation is reported. Human growth hormone (hGH) production and stability were tested under different heat shock conditions. Cell densities were 25 and 60 g l(-1) in a pH-stat fed-batch mode in defined and complex medium, respectively, and the fermentation time was decreased from 41 to 32 h. hGH was produced at 2 g l(-1) in complex medium. By using glycerol as main carbon source in the complex medium with exponential feeding, cell density and hGH production were increased to 100 g l(-1) and 2.7 g l(-1), respectively.
Tabandeh
Fatemeh
F
Fermentation Group, National Research Center for Genetic Engineering and Biotechnology, Tehran, Iran.
Shojaosadati
Sayed Abbas
SA
Zomorodipour
Alireza
A
Khodabandeh
Mahvash
M
Sanati
Mohammad H
MH
Yakhchali
Bagher
B
D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000502:physiology / Q000528:radiation effects; D003001:Cloning, Molecular; D015169:Colony Count, Microbial; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000254:growth & development* / Q000378:metabolism* / Q000528:radiation effects; D018014:Gene Transfer Techniques; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D018869:Heat-Shock Response / Q000502:physiology*; D006358:Hot Temperature; D019382:Human Growth Hormone / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D015202:Protein Engineering / Q000379:methods*; D011829:Radiation Dosage; D011994:Recombinant Proteins / Q000096:biosynthesis
D011994:Recombinant Proteins; D019382:Human Growth Hormone; D005947:Glucose; D005990:Glycerol