title: Preparation and a time-resolved fluoroimmunoassay application of new europium fluorescent nanoparticles.
abstract: New silica-based europium fluorescent nanoparticles having surface amino groups were prepared by a covalent binding-copolymerization technique. In the nanoparticles, the fluorescent Eu3+ chelate molecules were covalently bound to silicon atoms to protect the nanoparticles from dye leaking in bio-applications. The amino groups on the surface of nanoparticles made the surface modification and bioconjugation of nanoparticles easier. The nanoparticles were characterized and developed as a new type of fluorescence probe for a highly sensitive time-resolved fluoroimmunoassay (TR-FIA) of human hepatitis B surface antigen (HBsAg).
authors.affiliation: Department of Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012, PR China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Hai
authors.forename: Mingqian
authors.initials: M
authors.lastname: Tan
authors.forename: Guilan
authors.initials: G
authors.lastname: Wang
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Ye
authors.forename: Jingli
authors.initials: J
authors.lastname: Yuan
authors.forename: Kazuko
authors.initials: K
authors.lastname: Matsumoto