title: Heterochromatin and rDNA sites distribution in the holocentric chromosomes of Cuscuta approximata Bab. (Convolvulaceae).
abstract: Cuscuta is a widely distributed genus of holoparasitic plants. Holocentric chromosomes have been reported only in species of one of its subgenera (Cuscuta subg. Cuscuta). In this work, a representative of this subgenus, Cuscuta approximata, was investigated looking for its mitotic and meiotic chromosome behaviour and the heterochromatin distribution. The mitotic chromosomes showed neither primary constriction nor Rabl orientation whereas the meiotic ones exhibited the typical quadripartite structure characteristic of holocentrics, supporting the assumption of holocentric chromosomes as a synapomorphy of Cuscuta subg. Cuscuta. Chromosomes and interphase nuclei displayed many heterochromatic blocks that stained deeply with hematoxylin, 4',6-diamidino-2-phenylindole (DAPI), or after C banding. The banded karyotype showed terminal or subterminal bands in all chromosomes and central bands in some of them. The single pair of 45S rDNA sites was observed at the end of the largest chromosome pair, close to a DAPI band and a 5S rDNA site. Two other 5S rDNA site pairs were found, both closely associated with DAPI bands. The noteworthy giant nuclei of glandular cells of petals and ovary wall exhibited large chromocentres typical of polytenic nuclei. The chromosomal location of heterochromatin and rDNA sites and the structure of the endoreplicated nuclei of C. approximata seemed to be similar to those known in monocentric nuclei, suggesting that centromeric organization has little or no effect on chromatin organization.
authors.affiliation: Department of Botany, Federal University of Pernambuco, Pernambuco, Brazil. mguerra@ufpe.br
authors.forename: Marcelo
authors.initials: M
authors.lastname: Guerra
authors.forename: Miguel A
authors.initials: MA
authors.lastname: García
mesh_terms: D002455:Cell Division; D002871:Chromosome Banding; D032461:Chromosomes, Plant / Q000235:genetics*; D034182:Cuscuta / Q000166:cytology / Q000235:genetics*; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics*; D035263:Flowering Tops / Q000166:cytology*; D006570:Heterochromatin / Q000235:genetics*; D017403:In Situ Hybridization; D007621:Karyotyping; D008856:Microscopy, Fluorescence; D013030:Spain
chemical_list: D017931:DNA Primers; D004275:DNA, Ribosomal; D006570:Heterochromatin