[Study on the tumor cell targetability of folate-conjugated albumin nanoparticles].
OBJECTIVE
To study folate-conjugated albumin nanoparticles targeting to tumor cells via folate receptor-mediated endocytosis.


METHODS
The activated folic acid (N-hydroxysuccinimide ester of folic acid) was conjuated to the surface of bovine serum albumin nanoparticles (BSANP) via the amino groups. The extent of the influence that concentration, incubation time and free folate exerted on the uptake of Folate-BSANP by ovarian cancer cells (SKOV3) was determined using fluorescence spectrophotometer.


RESULTS
Folate-conjugated BSANP (Folate-BSANP) was successfully achieved. Uptake of Folate-BSANP by cancer cells was gradually increased with the extension of incubation time or the increase of Folate-BSANP concentration, and the uptake could be competitively inhibited by excess free folate.


CONCLUSIONS
Folate-BSANP could be delivered into tumor cells via folate receptor-mediated endocytosis and significantly targeted to tumor cells with rich folate receptors.
Zhang
Liang-ke
LK
Department of Pharmaceutics, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Hou
Shi-xiang
SX
Mao
Sheng-jun
SJ
Wei
Da-peng
DP
Song
Xiang-rong
XR
Qiao
Xiao-rong
XR
D000970:Antineoplastic Agents / Q000737:chemistry / Q000493:pharmacokinetics*; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics; D004337:Drug Carriers; D016503:Drug Delivery Systems; D058973:Folate Receptors, GPI-Anchored; D005492:Folic Acid / Q000737:chemistry / Q000493:pharmacokinetics*; D006801:Humans; D036103:Nanotechnology; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology; D011956:Receptors, Cell Surface / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000493:pharmacokinetics*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D002352:Carrier Proteins; D004337:Drug Carriers; D058973:Folate Receptors, GPI-Anchored; D011956:Receptors, Cell Surface; D012710:Serum Albumin, Bovine; D005492:Folic Acid