The effects of storage on fresh human osteochondral allografts.
Historically, fresh human osteochondral allografts have been stored in lactated Ringer's solution at 4 degrees C and then transplanted as quickly as possible, generally within 2 to 5 days, to ensure delivery of a high level of viable chondrocytes. Recently, allograft distribution companies have begun to provide fresh osteochondral allografts that are stored in a proprietary culture medium usually for at least 2 weeks before delivery to the surgeon for implantation. The effects of such storage on human cartilage have not been well-defined. In the current study the effects of storage in lactated Ringer's solution and in culture media were assessed. After 7 days of storage in lactated Ringer's solution, a significant decline in chondrocyte viability and metabolic activity was seen. Culture media provided significantly better preservation of the cartilage with viability and metabolic activity remaining essentially unchanged from baseline for as many as 14 days. The biochemical and biomechanical properties of the extracellular matrix remained stable with storage in both solutions with time. These data suggest that osteochondral allografts stored under traditional conditions in lactated Ringer's solution should continue to be implanted as quickly as possible and certainly within 7 days of donor death. If kept in culture media, the storage duration may be extended to approximately 2 weeks.
Ball
Scott T
ST
Department of Orthopaedics, University of California, San Diego, CA, USA.
Amiel
David
D
Williams
Seth K
SK
Tontz
William
W
Chen
Albert C
AC
Sah
Robert L
RL
Bugbee
William D
WD
D000328:Adult; D001696:Biomechanical Phenomena; D016025:Bone Transplantation / Q000502:physiology; D002356:Cartilage / Q000502:physiology / Q000637:transplantation*; D002470:Cell Survival; D019902:Chondrocytes / Q000378:metabolism / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D011509:Proteoglycans / Q000096:biosynthesis; D013997:Time Factors; D014021:Tissue Preservation
D011509:Proteoglycans