title: Isolation and characterization of site-specific DNA-methyltransferases from Bacillus coagulans K.
abstract: Two site-specific DNA methyltransferases, M.BcoKIA and M.BcoKIB, were isolated from the thermophilic strain Bacillus coagulans K. Each of the methylases protects the recognition site 5'-CTCTTC-3'/5'-GAAGAG-3' from cleavage with the cognate restriction endonuclease BcoKI. It is shown that M.BcoKIB is an N6-adenine specific methylase and M.BcoKIA is an N4-cytosine specific methylase. According to bisulfite mapping, M.BcoKIA methylates the first cytosine in the sequence 5'-CTCTTC-3'.
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russia.
authors.forename: I V
authors.initials: IV
authors.lastname: Svadbina
authors.forename: N V
authors.initials: NV
authors.lastname: Zelinskaya
authors.forename: N P
authors.initials: NP
authors.lastname: Kovalevskaya
authors.forename: L A
authors.initials: LA
authors.lastname: Zheleznaya
authors.forename: N I
authors.initials: NI
authors.lastname: Matvienko
mesh_terms: D000225:Adenine / Q000737:chemistry*; D001407:Bacillus / Q000201:enzymology*; D002853:Chromatography, Liquid; D003596:Cytosine / Q000737:chemistry*; D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000737:chemistry* / Q000302:isolation & purification*; D015257:DNA-Cytosine Methylases / Q000737:chemistry* / Q000302:isolation & purification*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry*; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000737:chemistry* / Q000302:isolation & purification*; D013379:Substrate Specificity
chemical_list: D003596:Cytosine; D015254:DNA Modification Methylases; D015257:DNA-Cytosine Methylases; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); C057073:endodeoxyribonuclease Ksp632I; D015252:Deoxyribonucleases, Type II Site-Specific; D000225:Adenine