title: Effect of amoxicillin-clavulanic acid on human fecal flora in a gnotobiotic mouse model assessed with fluorescence hybridization using group-specific 16S rRNA probes in combination with flow cytometry.
abstract: Predominant groups of bacteria from a human fecal flora-associated mouse model challenged with amoxicillin-clavulanic acid were quantified with fluorescence in situ hybridization combined with flow cytometry using specific 16S rRNA targeted oligonucleotide probes. This approach provides a useful tool with high throughput to evaluate fecal microflora under antibiotic treatment.
authors.affiliation: Unité associée INRA Ecosystème Microbien Digestif et Santé, Faculté de Pharmacie, Université Paris-Sud, 92296 Châtenay-Malabry Cedex, France.
authors.forename: Marie Claude
authors.initials: MC
authors.lastname: Barc
authors.forename: François
authors.initials: F
authors.lastname: Bourlioux
authors.forename: Lionel
authors.initials: L
authors.lastname: Rigottier-Gois
authors.forename: Céline
authors.initials: C
authors.lastname: Charrin-Sarnel
authors.forename: Claire
authors.initials: C
authors.lastname: Janoir
authors.forename: Hélène
authors.initials: H
authors.lastname: Boureau
authors.forename: Joël
authors.initials: J
authors.lastname: Doré
authors.forename: Anne
authors.initials: A
authors.lastname: Collignon
mesh_terms: D019980:Amoxicillin-Potassium Clavulanate Combination / Q000494:pharmacology*; D000818:Animals; D001421:Bacteria, Anaerobic / Q000187:drug effects; D015169:Colony Count, Microbial; D004359:Drug Therapy, Combination / Q000494:pharmacology*; D005243:Feces / Q000382:microbiology*; D005260:Female; D005434:Flow Cytometry; D005856:Germ-Free Life; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008809:Mice, Inbred C3H; D015345:Oligonucleotide Probes; D015347:RNA Probes; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
chemical_list: D015345:Oligonucleotide Probes; D015347:RNA Probes; D012336:RNA, Ribosomal, 16S; D019980:Amoxicillin-Potassium Clavulanate Combination