Immunization of Saimiri sciureus monkeys with Plasmodium falciparum merozoite surface protein-3 and glutamate-rich protein suggests that protection is related to antibody levels.
The immunogenicity and protective efficacy of various antigen-adjuvant formulations derived either from the merozoite-surface protein-3 (MSP-3) or the glutamate-rich protein (GLURP) of Plasmodium falciparum were evaluated in Saimiri sciureus monkeys. These proteins were selected for immunogenicity studies based primarily on their capacity of inducing an antibody-dependent cellular inhibition effect on parasite growth. Some of the S. sciureus monkeys immunized with MSP-3(212-380)-AS02 or GLURP(27-500)-alum were able to fully or partially control parasitaemia upon an experimental P. falciparum [Falciparum Uganda Palo Alto (FUP-SP) strain] blood-stage infection, and this protection was related to the prechallenge antibody titres induced. The data are indicative that MSP-3 and GLURP can induce protective immunity against an experimental P. falciparum infection using adjuvants that are acceptable for human use and this should trigger further studies with those new antigens.
Carvalho
L J M
LJ
Laboratory of Malaria Research, Department of Immunology, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil. leojmc@ioc.fiocruz.br
Oliveira
S G
SG
Theisen
M
M
Alves
F A
FA
Andrade
M C R
MC
Zanini
G M
GM
Brígido
M C O
MC
Oeuvray
C
C
Póvoa
M M
MM
Muniz
J A P C
JA
Druilhe
P
P
Daniel-Ribeiro
C T
CT
D000818:Animals; D000906:Antibodies / Q000097:blood* / Q000276:immunology; D000953:Antigens, Protozoan / Q000008:administration & dosage / Q000276:immunology / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D007156:Immunologic Memory / Q000187:drug effects / Q000276:immunology; D017780:Malaria Vaccines / Q000008:administration & dosage / Q000276:immunology / Q000494:pharmacology*; D016778:Malaria, Falciparum / Q000517:prevention & control*; D010455:Peptides / Q000008:administration & dosage / Q000276:immunology / Q000494:pharmacology; D010963:Plasmodium falciparum / Q000276:immunology*; D015800:Protozoan Proteins / Q000008:administration & dosage / Q000276:immunology / Q000494:pharmacology*; D012453:Saimiri
D000906:Antibodies; D000953:Antigens, Protozoan; D017780:Malaria Vaccines; D010455:Peptides; D015800:Protozoan Proteins; C089133:merozoite surface protein 3, Plasmodium; C072492:glutamate-rich protein, Plasmodium