title: Betagamma subunits mediate the NPY enhancement of ATP-stimulated inositol phosphate formation.
abstract: Neuropeptide Y (NPY) enhances ATP-stimulated inositol phosphate (InsP) formation in bovine chromaffin cells through an unknown mechanism. Chromaffin cells were transduced with the carboxyl terminus of beta-adrenergic receptor kinase 1 (betaARK1CT), a Gbetagamma subunits scavenger, using a recombinant adenovirus system. The adenovirus also expresses a green fluorescent protein (GFP) which serves as an index of transduction. Flow cytometry showed that up to 80% of chromaffin cells were transduced by the virus. There was a direct correlation between the betaARK1CT inhibition of the NPY enhancement of ATP-stimulated InsP formation and the percent of cells expressing GFP ( r2=0.9993 ). These results demonstrate that Gbetagamma subunits are required for the NPY enhancement of ATP-stimulated InsP formation in bovine chromaffin cells.
authors.affiliation: Department of Pharmacology, 986260 Nebraska Medical Center, University of Nebraska Medical Center, Omaha, NE 68198-6260, USA.
authors.forename: Xinying
authors.initials: X
authors.lastname: Li
authors.forename: Tsuneya
authors.initials: T
authors.lastname: Ikezu
authors.forename: Terry D
authors.initials: TD
authors.lastname: Hexum
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D019439:Chromaffin Cells / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D007295:Inositol Phosphates / Q000378:metabolism*; D008164:Luminescent Proteins / Q000378:metabolism; D009478:Neuropeptide Y / Q000378:metabolism*; D051552:beta-Adrenergic Receptor Kinases
chemical_list: D007295:Inositol Phosphates; D008164:Luminescent Proteins; D009478:Neuropeptide Y; D049452:Green Fluorescent Proteins; D000255:Adenosine Triphosphate; D017868:Cyclic AMP-Dependent Protein Kinases; D051552:beta-Adrenergic Receptor Kinases