title: Construction of a glucose sensor based on a screen-printed electrode and a novel mediator pyocyanin from Pseudomonas aeruginosa.
abstract: Pyocyanin is the blue phenazine pigment produced by Pseudomonas aeruginosa. Pyocyanin production using immobilized cells was investigated. The maximum production of pyocyanin was obtained using cells immobilized in kappa-carrageenan. Moreover, 0.01% PO4(3-), 0.2% Mg(2+), 0.001% Fe(2+), 1% glycerine, 0.8% leucine and 0.8% dl-alanine were also essential for pyocyanin production. Pyocyanin was purified by chloroform extraction and silica gel column chromatography. An amperometric biosensor system using a screen-printed electrode and pyocyanin as mediator were also developed for a more accurate determination of glucose concentration. Pyocyanin, which exists in the oxidated form, was reduced by the reaction between glucose oxidase and glucose. The reduced form was then converted back to the oxidized form by an oxidative reaction on the electrode. There was a linear relation ship between sensor output currents and glucose concentrations ranging from 1 to 20mM under the following conditions: -200 mV of the applied potential, pH 5.0, and 10 U of the immobilized enzyme. The coefficient of variation was below 3% (n = 5) for the glucose sensor.
authors.affiliation: Depertment of Food Science and Technology, Tokyo University of Fisheries, 5-7 Kounan 4, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Ohfuji
authors.forename: Naruhide
authors.initials: N
authors.lastname: Sato
authors.forename: Naoko
authors.initials: N
authors.lastname: Hamada-Sato
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Chiaki
authors.initials: C
authors.lastname: Imada
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Okuma
authors.forename: Etsuo
authors.initials: E
authors.lastname: Watanabe
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004566:Electrodes; D005947:Glucose / Q000032:analysis*; D011550:Pseudomonas aeruginosa; D011710:Pyocyanine / Q000302:isolation & purification
chemical_list: D011710:Pyocyanine; D005947:Glucose