Interaction of Leishmania (L.) chagasi with the Vero cell line.
The Vero cell line, a non-phagocytic cell, has supported the intracellular mechanism of Leishmania (L.) chagasi. This strain (MHOM/BR/501/MS00) was isolated from a human case of visceral leishmaniasis in Mato Grosso do Sul, Brazil and cultivated in Schneider's Drosophila medium with 20% of heat inactivated fetal calf serum. It was allowed to infect the Vero cells at a ratio of 10 to 20 promastigotes per cell. Within six hours of incubation, promastigote forms were found attached to Vero cells without any particular orientation. The number of amastigotes per cell increased during the incubation period. Results showed that promastigotes of L. (L.) chagasi could interact, transform to amastigote forms and multiply in non-phagocytic cells, demonstrating a new model to study the intracellular cycle of this protozoan.
Pessotti
J H
JH
Laboratório de Imunomodulação, Departamento de Protozoologia, Instituto Oswaldo Cruz, Av. Brasil 4365, Manguinhos, 21045-900, Fiocruz, Rio de Janeiro, Brasil.
Zaverucha Do Valle
T
T
Corte-Real
S
S
Gonçalves Da Costa
S C
SC
D000818:Animals; D002522:Chlorocebus aethiops; D003470:Culture Media; D018314:Leishmania infantum / Q000254:growth & development / Q000502:physiology*; D008854:Microscopy, Electron / Q000662:veterinary; D014709:Vero Cells / Q000469:parasitology* / Q000648:ultrastructure
D003470:Culture Media