title: Degeneration of beta-glucosidase activity in a foam fractionation process.
abstract: Foam fractionation is a promising technique for concentrating proteins because of its simplicity and low operating cost. One such protein that can be foamed is the enzyme cellulase. The use of inexpensively purified cellulase may be a key step in the economical production of ethanol from biomass. We conducted foam fractionation experiments at total reflux using the cellulase component beta-glucosidase to study how continuous shear affects beta-glucosidase in a foam such as a fermentation or foam fractionation process. The experiments were conducted at pH 2.4, 5.4, and 11.6 and airflow rates of 3, 6, 15, 20, and 32 cc/min to determine how beta-glucosidase activity changes in time at these different conditions. This is apparently a novel and simple way of testing for changes in enzyme activity within a protein foam. The activity did not degenerate during 5 min of reflux at pH 5.4 at an airflow rate of 10 cc/min. It was established that at 10 min of refluxing, the beta-glucosidase denatured more as the flow rate increased. At pH 2.4 and a flow rate of 10 cc/min, the activity remained constant for at least 15 min.
authors.affiliation: Department of Chemical Engineering, Vanderbilt University, Station B, PO Box 351604, Nashville, TN 37235, USA.
authors.forename: Vorakan
authors.initials: V
authors.lastname: Burapatana
authors.forename: Ales
authors.initials: A
authors.lastname: Prokop
authors.forename: Robert D
authors.initials: RD
authors.lastname: Tanner
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000737:chemistry*; D005959:Glucosidases / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D013997:Time Factors; D001617:beta-Glucosidase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005959:Glucosidases; D001617:beta-Glucosidase; D002480:Cellulase