Effect of polysaccharides and human plasma lipoproteins on the secretion of cystatin C by peritoneal macrophages from normal and tumor bearing mice.
Intact peritoneal macrophages in vitro secreted the cysteine proteinase inhibitor cystatin C. Polysaccharides stimulated cystatin C secretion: lipopolysaccharide < carboxymethylated beta-D-glucan < sulfoethylated beta-D-glucan. Human plasma low-density- (LDL) and high-density lipoproteins (HDL) are still more potent inducers of cystatin C secretion by macrophages. Peritoneal macrophages from mice with experimental HA-1 hepatoma compared to those from intact mice secreted more cystatin C with maximum polysaccharide-stimulated secretion after 30 min of incubation. LDL and HDL induced cystatin C secretion by tumor macrophages also.
Poteryaeva
O N
ON
Institute of Biochemistry, Siberian Branch of the Russian Academy of Medical Sciences, Novosibirsk 630117, Russia. ibch@soramn.ru
Polyakov
L M
LM
Korolenko
T A
TA
Zueva
T V
TV
Russkikh
G S
GS
Panin
L E
LE
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D055316:Cystatin C; D015891:Cystatins / Q000096:biosynthesis*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D008074:Lipoproteins / Q000494:pharmacology*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008297:Male; D051379:Mice; D010949:Plasma; D011134:Polysaccharides / Q000494:pharmacology*
C525030:CST3 protein, human; C525031:Cst3 protein, mouse; D055316:Cystatin C; D015891:Cystatins; D008074:Lipoproteins; D011134:Polysaccharides