Influence of routes and administration parameters on antibody response of pigs following DNA vaccination.
Using the nucleoprotein of porcine reproductive and respiratory syndrome virus as model antigen, we optimised parameters for gene gun vaccination of pigs, including firing pressure and vaccination site. As criteria for optimisation, we characterised particle penetration and local tissue damage by histology. For selected combinations, vaccination efficiency in terms of antibody response was studied. Gene gun vaccination on ear alone was as efficient as a multi-site (ear, thorax, inguinal area, tongue mucosa) gene gun approach, and more efficient than combined intramuscular (i.m.)/intradermal (i.d.) injection of plasmid DNA. This indicates, that the ear is an attractive site for gene gun vaccination of pigs.
Barfoed
Annette Malene
AM
Laboratory of Virology and Immunology, Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Stigbøjlen 7, DK-1870 Frederiksberg C, Denmark.
Kristensen
Birte
B
Dannemann-Jensen
Tove
T
Viuff
Birgitte
B
Bøtner
Anette
A
Kamstrup
Søren
S
Blixenkrone Møller
Merete
M
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis; D019470:Biolistics; D004247:DNA / Q000096:biosynthesis / Q000276:immunology; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D006046:Gold / Q000493:pharmacokinetics; D007271:Injections, Intradermal; D007273:Injections, Intramuscular; D051379:Mice; D009092:Mucous Membrane / Q000276:immunology; D009698:Nucleoproteins / Q000276:immunology; D010316:Particle Size; D010957:Plasmids / Q000235:genetics / Q000276:immunology; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D013552:Swine; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000914:Antibodies, Viral; D017931:DNA Primers; D009698:Nucleoproteins; D019444:Vaccines, DNA; D014765:Viral Vaccines; D006046:Gold; D004247:DNA