The role of disulfide bonds in the conformational stability and catalytic activity of phytase.
Previous studies have predicted five disulfide bonds in Aspergillus niger phytase (phy A). To investigate the role of disulfide bonds, intrinsic fluorescence spectra, far-ultraviolet circular dichroism (CD) spectra, and an enzyme activity assay were used to compare the differences of catalytic activity and conformational stability of phytase during denaturation in urea in the presence and absence of dithiothreitol (DTT). In the presence of 2 mM DTT, the inactivation and unfolding were greatly enhanced at the same concentration of denaturant. The fluorescence emission maximum red shift and decreases of ellipticity at 222 nm were in accord with the changes of catalytic activity. The kinetics of the unfolding courses were a biphasic process consisting of two first-order reactions in the absence of DTT and a monophasic process of a first-order reaction in the presence of DTT. The results suggested that the loss of enzymatic activity was most likely because of a conformational change, and that disulfide bonds played an important role in three-dimensional structure and catalytic activity.
Wang
Xiao-Yun
XY
College of Life Science, Shandong Agricultural University, Shandong Tai'an, People's Republic of China.
Meng
Fan-Guo
FG
Zhou
Hai-Meng
HM
D010832:6-Phytase / Q000737:chemistry* / Q000378:metabolism*; D002384:Catalysis; D002942:Circular Dichroism; D004220:Disulfides / Q000737:chemistry / Q000378:metabolism; D004229:Dithiothreitol; D011487:Protein Conformation; D011489:Protein Denaturation; D014508:Urea
D004220:Disulfides; D014508:Urea; D010832:6-Phytase; D004229:Dithiothreitol