title: Prostaglandins are powerful inducers of NGF and BDNF production in mouse astrocyte cultures.
abstract: We found that prostaglandin (PG) D(2) and PGE(2), which are major PGs in the brain of mammals, powerfully induced the secretion of nerve growth factor (NGF) from cultured mouse astrocytes; PGE(2) or PGD(2) induced an approximately 12- or 19-fold increase in NGF secretion after a 24-h incubation, respectively. Moreover, it was found that the sequential metabolites of PGD(2), PGJ(2), Delta(12)-PGJ(2), and 15-deoxy-Delta(12,14)-PGJ(2), induced the NGF secretion to the culture medium strikingly (60-98-fold of the control after a 24-h incubation). NGF secretion induced by the J(2) series of PGs was accompanied by the increased expression of NGF mRNA. These PGs also stimulated the secretion/synthesis of brain-derived neurotrophic factor (BDNF). Our findings suggest that PGs play a neuroprotective role by inducing NGF and BDNF production in the central nervous system.
authors.affiliation: Department of Biochemistry, Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka 569-1094, Japan.
authors.forename: Misao
authors.initials: M
authors.lastname: Toyomoto
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Ohta
authors.forename: Kohji
authors.initials: K
authors.lastname: Okumura
authors.forename: Hiroe
authors.initials: H
authors.lastname: Yano
authors.forename: Kaori
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Seiji
authors.initials: S
authors.lastname: Inoue
authors.forename: Kyozo
authors.initials: K
authors.lastname: Hayashi
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ikeda
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism*; D051379:Mice; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism*; D015230:Prostaglandin D2 / Q000031:analogs & derivatives* / Q000235:genetics / Q000378:metabolism*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; C037112:9-deoxy-delta-9-prostaglandin D2; D020932:Nerve Growth Factor; D015232:Dinoprostone; D015230:Prostaglandin D2