An HPLC-MS approach for analysis of very long chain fatty acids and other apolar compounds on octadecyl-silica phase using partly miscible solvents.
A novel approach for analyzing underivatized very long chain fatty acids (C16-C26) and other apolar compounds such as triacylglycerols is described. It is based on reversed-phase HPLC separation followed by mass spectrometric detection. Partly miscible solvents are used for stepwise gradient elution starting with a methanol/water and ending with a methanol/n-hexane binary mixture. The developed technique does not need derivatization, and analysis is fast (fatty acids were separated in 2-min-long chromatograms) and robust. The developed method is also very sensitive; a quantitation limit in the low-picogram range was achieved for fatty acids. The separation mechanism and advantages of the suggested technique are discussed and illustrated in the case of blood analysis and plant oil characterization.
Nagy
Kornél
K
Institute of Chemistry, Chemical Research Center, Hungarian Academy of Sciences, H-1025 Pusztaszeri út 59-67, Budapest, Hungary.
Jakab
Annamária
A
Fekete
Jeno
J
Vékey
Károly
K
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005227:Fatty Acids / Q000097:blood* / Q000737:chemistry; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D010938:Plant Oils / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D014280:Triglycerides / Q000032:analysis
D005227:Fatty Acids; D010938:Plant Oils; D012997:Solvents; D014280:Triglycerides; C026679:octadecylsilica; C068582:almond oil; D012822:Silicon Dioxide; C017364:hexacosanoic acid; C007547:behenic acid; C010210:lignoceric acid