title: The novel organization and complete sequence of the ribosomal RNA gene of Nosema bombycis.
abstract: We present here for the first time the complete DNA sequence data (4301bp) of the ribosomal RNA (rRNA) gene of the microsporidian type species, Nosema bombycis. Sequences for the large subunit gene (LSUrRNA: 2497bp, GenBank Accession No. ), the internal transcribed spacer (ITS: 179bp, GenBank Accession No. ), the small subunit gene (SSUrRNA: 1232bp), intergenic spacer (IGS: 279bp), and 5S region (114bp) are also given, and the secondary structure of the large subunit is discussed. The organization of the N. bombycis rRNA gene is LSUrRNA-ITS-SSUrRNA-IGS-5S. This novel arrangement, in which the LSU is 5' of the SSU, is the reverse of the organizational sequence (i.e., SSU-ITS-LSU) found in all previously reported microsporidian rRNAs, including Nosema apis. This unique character in the type species may have taxonomic implications for the members of the genus Nosema.
authors.affiliation: Department of Entomology, National Taiwan University, Taipei 106, Taiwan, ROC.
authors.forename: Wei-Fone
authors.initials: WF
authors.lastname: Huang
authors.forename: Shu-Jen
authors.initials: SJ
authors.lastname: Tsai
authors.forename: Chu-Fang
authors.initials: CF
authors.lastname: Lo
authors.forename: Yamane
authors.initials: Y
authors.lastname: Soichi
authors.forename: Chung-Hsiung
authors.initials: CH
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D017125:Genes, Protozoan; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D016818:Nosema / Q000235:genetics*; D009690:Nucleic Acid Conformation; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer