Liquid chromatography-hydride generation-atomic absorption spectrometry for the speciation of tin in seafoods.
Liquid chromatography with hydride generation atomic absorption spectrometry as the detection system was applied to the separation and determination of inorganic tin, tributyltin, dibutyltin, monobutyltin, diphenyltin and monophenyltin. A reversed phase C18 column and a methanol/water/acetic acid (70:27:3, v/v/v) mixture containing 0.05%(v/v) triethylamine and 0.1%(w/v) tropolone as the mobile phase (isocratic elution) were used. Extraction of organotins from the samples was carried out using methanol containing 0.05%(w/v) tropolone, a process that was repeated twice. The supernatants were shaken with water and dichloromethane in a separating funnel and the organic phase was collected and evaporated to dryness. When the method was applied to the speciation of tin in fresh and canned mussels, no organotins above the detection limits were identified in any of the samples, inorganic tin being the only species detected. The reliability of the procedure was checked by analyzing the total tin content of the samples by electrothermal atomic absorption spectrometry and by speciation of tin in a certified reference material, mussel tissue (CRM 477). The method can be used for environmental monitoring of organotins contaminated samples.
Viñas
Pilar
P
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, E-30071 Murcia, Spain.
López-García
Ignacio
I
Merino-Meroño
Beatriz
B
Campillo
Natalia
N
Hernández-Cordóba
Manuel
M
D002853:Chromatography, Liquid; D005506:Food Contamination; D006801:Humans; D009947:Organotin Compounds / Q000032:analysis*; D015203:Reproducibility of Results; D018570:Risk Assessment; D017747:Seafood; D013054:Spectrophotometry, Atomic; D014001:Tin / Q000032:analysis*
D009947:Organotin Compounds; D014001:Tin