G and P genotypes of group A rotavirus strains circulating in calves in Brazil, 1996-1999.
Fifty fecal samples from calves with diarrhea, positive for group A rotavirus by enzyme immunoassay (EIA) and polyacrylamide gel electrophoresis (PAGE) were analyzed by a nested multiplex reverse transcription/polymerase chain reaction (Nested multiplex/RT-PCR) for identification of P and G genotypes. Samples were collected between 1996 and 1999 from eight dairy and/or beef cattle herds located in the Mato Grosso do Sul, São Paulo, Goiás and Paraná States, Brazil. Complete genotyping was possible in 44 (88%) of the calf fecal samples. The VP7 gene could not be identified in six (12%) of the samples. Whilst the VP4 (P) gene was identified in 100% of samples. The genotypes of 35 (70%) samples corresponded to the NCDV-like (12%), UK-like (40%), B223-like (16%) and KN-4 (2%) reference rotavirus strains, which are commonly found in cattle. Mixed infections were detected in seven (14%) samples, and genotypes observed in two (4%) samples presented unusual combinations, carrying VP4 and VP7 gene of the bovine group A rotavirus (P[11],G8), in other one carrying a VP4 gene of bovine origin (P[1]) and a VP7 gene of swine origin (G5).
Alfieri
Alice Fernandes
AF
Laboratory of Animal Virology, Department of Preventive Veterinary Medicine, Londrina State University, Londrina, PR, Brazil.
Alfieri
Amauri Alcindo
AA
Barreiros
Marco Antônio Bacellar
MA
Leite
José Paulo Gagliardi
JP
Richtzenhain
Leonardo José
LJ
D000818:Animals; D001938:Brazil; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000821:virology*; D003967:Diarrhea / Q000662:veterinary / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005243:Feces / Q000821:virology; D005838:Genotype; D007124:Immunoenzyme Techniques / Q000662:veterinary; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012401:Rotavirus / Q000235:genetics*; D012400:Rotavirus Infections / Q000097:blood / Q000662:veterinary* / Q000821:virology
D012367:RNA, Viral