title: Differential ability of polymorphic OGG1 proteins to suppress mutagenesis induced by 8-hydroxyguanine in human cell in vivo.
abstract: OGG1 protein has an ability to suppress mutagenesis induced by 8-hydroxyguanine (8OHG), an oxidatively damaged promutagenic base. Here, the mutation suppressive ability was compared between two common polymorphic OGG1 proteins, OGG1-Ser326 and OGG1-Cys326, using a supF forward mutation assay employing an 8OHG-containing plasmid. Polymorphic OGG1 proteins were exogenously expressed by adenoviral transduction in H1299 human lung cancer cells, in which endogenous OGG1 protein was undetectable by western blot analysis. Mutations by 8OHG were more efficiently suppressed in OGG1-Ser326 transduced cells than OGG1-Cys326 transduced cells. The results indicated that OGG1-Cys326 has a lower ability to prevent mutagenesis by 8OHG than OGG1-Ser326 in vivo in human cells; supporting the results of recent association studies that OGG1-Cys326 is a risk allele for several types of human cancers.
authors.affiliation: Biology Division, National Cancer Center Research Institute, 1-1 Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan.
authors.forename: Arito
authors.initials: A
authors.lastname: Yamane
authors.forename: Takashi
authors.initials: T
authors.lastname: Kohno
authors.forename: Kohei
authors.initials: K
authors.lastname: Ito
authors.forename: Noriaki
authors.initials: N
authors.lastname: Sunaga
authors.forename: Kazunori
authors.initials: K
authors.lastname: Aoki
authors.forename: Kimio
authors.initials: K
authors.lastname: Yoshimura
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Murakami
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Nojima
authors.forename: Jun
authors.initials: J
authors.lastname: Yokota
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D015153:Blotting, Western; D045647:DNA Glycosylases / Q000502:physiology*; D016153:Genes, Suppressor; D006147:Guanine / Q000009:adverse effects* / Q000031:analogs & derivatives*; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology / Q000235:genetics*; D016296:Mutagenesis; D009154:Mutation / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics*; D012343:RNA, Transfer / Q000502:physiology; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: C096275:supF tRNA; C024829:8-hydroxyguanine; D006147:Guanine; D012343:RNA, Transfer; D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human