title: Immunohistochemical characterization of the leucocytic infiltrate associated with canine seminomas.
abstract: Both canine and human seminomas are typically associated with leucocytic infiltration, the role of which remains poorly understood. In this study, leucocytes infiltrating 10 canine seminomas were characterized. Monoclonal antibodies directed against CD18, CD11a, CD11b, CD11c, CD21, CD3, CD4, CD8 and Major Histocompatibility Complex class I and II (MHC I and MHC II) were also employed. Infiltrating leucocytes were located around vessels, adjacent to the thin fibrous septa between neoplastic lobules, and were also scattered singly amongst neoplastic cells. The more profuse infiltrates often had the appearance of follicular aggregates. Immunohistochemically, all the samples showed generally similar results. Most of the infiltrating cells were positive for CD18 and CD11a. Infiltrating cells were mainly T lymphocytes (CD3+), particularly of the CD8+ subset. B lymphocytes (CD21+) were detectable in almost all infiltrates; in the follicular aggregates they were centrally located, whereas T lymphocytes (CD3+) lined the periphery. CD11c+ cells were always more numerous than CD11b+ cells, demonstrating that if macrophages and antigen-presenting cells (APCs) were well represented, monocytes and granulocytes were practically absent. Almost all of the infiltrating cells were positive for both MHC I and MHC II antigens and, in nine samples, a large number of neoplastic cells expressing MHC I were detected. A few neoplastic cells expressing MHC II were observed in seven cases. The presence of CD8+ lymphocytes, together with the large number of both infiltrating and neoplastic cells expressing MHC I, suggests that the lymphocytes mediate a cytotoxic reaction against the neoplastic cells. This hypothesis may underlie the favourable prognosis frequently associated with canine seminomas.
authors.affiliation: Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Sezione di Anatomia Patologica Veterinaria e Patologia Aviare, Facolta di Medicina veterinaria, Via Celoria 10, 20133 Milano, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Grieco
authors.forename: M
authors.initials: M
authors.lastname: Rondena
authors.forename: S
authors.initials: S
authors.lastname: Romussi
authors.forename: D
authors.initials: D
authors.lastname: Stefanello
authors.forename: M
authors.initials: M
authors.lastname: Finazzi
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D004285:Dogs; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007150:Immunohistochemistry; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D018239:Seminoma / Q000378:metabolism* / Q000473:pathology*; D013736:Testicular Neoplasms / Q000378:metabolism* / Q000473:pathology*
chemical_list: D015703:Antigens, CD; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II