title: Proteomic analysis on symbiotic differentiation of mitochondria in soybean nodules.
abstract: Symbiotic interactions between legume plants and rhizobia induce specific metabolisms and intracellular organelles in nodules. For surveying symbiotic differentiation of a key organelle, mitochondria, protein constituents of soybean nodule and root mitochondria were compared after two-dimensional (2-D) electrophoresis, and the proteins were characterized in combination with matrix-assisted desorption/ionization time-of-flight mass spectrometry, electrospray ionization mass spectrometry and N-terminal amino acid sequencing. Of the proteins that were detected only in nodule mitochondria, phosphoserine aminotransferase, flavanone 3-hydroxylase, coproporphyrinogen III oxidase, one ribonucleoprotein and three unknown proteins were identified. Seven up-regulated, eight down-regulated and two strongly suppressed protein spots in nodule mitochondria were also assigned protein identities. The physiological roles of these differential expressions were discussed in relation to nodule-specific metabolisms in soybean nodules.
authors.affiliation: Department of Life Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa, 761-0795 Japan.
authors.forename: Le Thi-Phuong
authors.initials: le TP
authors.lastname: Hoa
authors.forename: Mika
authors.initials: M
authors.lastname: Nomura
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Kajiwara
authors.forename: David Alexander
authors.initials: DA
authors.lastname: Day
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Tajima
mesh_terms: D000595:Amino Acid Sequence; D002454:Cell Differentiation / Q000502:physiology; D015180:Electrophoresis, Gel, Two-Dimensional; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D024101:Mitochondrial Proteins / Q000032:analysis* / Q000737:chemistry; D010940:Plant Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D018517:Plant Roots / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development; D020543:Proteome / Q000032:analysis* / Q000235:genetics; D020539:Sequence Analysis, Protein; D013025:Glycine max / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013559:Symbiosis / Q000502:physiology
chemical_list: D024101:Mitochondrial Proteins; D010940:Plant Proteins; D020543:Proteome