title: Mutation scanning analysis of Marteilia sydneyi populations from different geographical locations in eastern Australia.
abstract: Marteilia sydneyi (Paramyxea) is the causative agent of QX disease in oysters. In spite of the economic impact of this disease, its origin and the precise reason(s) for its apparent spread in Australian waters are not yet known. Given such knowledge gaps, investigating the population genetic structure(s) of M. sydneyi populations could provide insights into the epidemiology and ecology of the parasite and could assist in its prevention and control. In this study, single strand conformation polymorphism (SSCP)-based analysis of a region (195 bp) of the first internal transcribed spacer (ITS-1) of ribosomal DNA was employed to investigate genetic variation within and among five populations of M. sydneyi from oysters from five different locations in eastern Australia. The analysis showed the existence of a genetic variant of M. sydneyi common to the Great Sandy Strait, and the Richmond and Georges Rivers, as distinct from variants at the Pimpama and Clarence Rivers. Together with historical and other information relating to the QX disease outbreaks in eastern Australia, the molecular findings support the proposal that the parasite originated in the Great Sandy Strait and/or Richmond River and then extended southward along the coast. From a technical perspective, the study demonstrated the usefulness of SSCP as a tool to study the population genetics and epidemiology of M. sydneyi.
authors.affiliation: Department of Microbiology and Parasitology, University of Queensland, Brisbane, Qld 4072, Australia.
authors.forename: Sarah N
authors.initials: SN
authors.lastname: Kleeman
authors.forename: Robert D
authors.initials: RD
authors.lastname: Adlard
authors.forename: Xingquan
authors.initials: X
authors.lastname: Zhu
authors.forename: Robin B
authors.initials: RB
authors.lastname: Gasser
mesh_terms: D000818:Animals; D001315:Australia; D001483:Base Sequence; D016172:DNA Fingerprinting; D004252:DNA Mutational Analysis / Q000379:methods*; D004275:DNA, Ribosomal / Q000235:genetics; D056890:Eukaryota / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010125:Ostreidae / Q000469:parasitology*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011153:Population / Q000235:genetics
chemical_list: D004275:DNA, Ribosomal