title: Molecularly imprinted sol-gel nanotubes membrane for biochemical separations.
abstract: In this study, we report a simple procedure for applying molecular imprinting functional groups to the inner surfaces of the template-synthesized sol-gel nanotubes for chemical separation of estrone. The silica nanotubes were synthesized within the pores of nanopore alumina template membranes using a sol-gel method by simultaneous hydrolysis of a silica monomer-imprinted molecule complex and tetraethoxysilane (TEOS). A covalent imprinting strategy was employed by generating a sacrificial spacer through the reaction of the isocyanate group of 3-(triethoxysilyl)propyl isocyanate and a phenol moiety of estrone to form a thermally cleavable urethane bond. This allowed us to remove the imprinted estrone by simple thermal reaction and to simultaneously introduce functional groups into the cavity formed by the silica nanotubes. Experiments indicated that estrone could be bound selectively by such an approach and have a binding affinity of 864 +/- 137 (n = 3).
authors.affiliation: The First Institute of Oceanography, SOA, Qingdao, P. R. China. hhyang@yanan.xmu.edu.cn
authors.forename: Huang-Hao
authors.initials: HH
authors.lastname: Yang
authors.forename: Shu-Qiong
authors.initials: SQ
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.forename: Xiao-Lan
authors.initials: XL
authors.lastname: Chen
authors.forename: Zhi-Xia
authors.initials: ZX
authors.lastname: Zhuang
authors.forename: Jin-Gou
authors.initials: JG
authors.lastname: Xu
authors.forename: Xiao-Ru
authors.initials: XR
authors.lastname: Wang
mesh_terms: D004970:Estrone / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D005260:Female; D005782:Gels / Q000737:chemistry; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D036103:Nanotechnology; D043942:Nanotubes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties; D013696:Temperature; D013739:Testosterone / Q000737:chemistry / Q000378:metabolism
chemical_list: D005782:Gels; D008567:Membranes, Artificial; D004970:Estrone; D013739:Testosterone; D012822:Silicon Dioxide