title: [Sampling method adapted for colonial cyanobacteria in a lake environment. Case study of Microcystis aeruginosa in the Grangent reservoir (Loire, France)].
abstract: New method of sampling adapted to colonial cyanobacteria was developed on the Grangent reservoir (Loire, France). These prokaryotes were sampled using a filtering pump and were counted at laboratory under epifluorescence microscope. This method allowed us to follow the annual cycle of Microcystis aeruginosa, since benthic spring recruitment (cyanobacteria being used as inoculum) until autumnal sedimentation, and even revealed the presence of this cyanobacterian species in winter in the epilimnion. Our study also showed the inadequacy of the standard sampling methods used in limnology, which badly underestimate colonial cyanobacteria.
authors.affiliation: Laboratoire de biologie animale et appliquée, faculté des sciences et techniques, université Jean-Monnet, 23, rue du Docteur-Paul-Michelon, 42023 Saint-Etienne, France. delphine.latour@univ-st-etienne.fr
authors.forename: Delphine
authors.initials: D
authors.lastname: Latour
authors.forename: Hervé
authors.initials: H
authors.lastname: Giraudet
authors.forename: Marie-José
authors.initials: MJ
authors.lastname: Salençon
mesh_terms: D015169:Colony Count, Microbial / Q000295:instrumentation / Q000379:methods*; D005374:Filtration; D005602:France; D005618:Fresh Water / Q000382:microbiology*; D046931:Microcystis / Q000302:isolation & purification* / Q000502:physiology; D008856:Microscopy, Fluorescence; D010839:Phytoplankton; D012621:Seasons; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods*; D014871:Water Microbiology