Role of capsaicin-sensitive afferent nerves in different models of gastric inflammation in rats.
Capsaicin-sensitive afferent nerves are described as being protective against gastric inflammation; their destruction leads to an exacerbation of inflammatory processes. However, these nerves have been shown to exert a pro-inflammatory action on stress-induced gastritis in rats. Our study aimed to investigate the role of capsaicin-sensitive afferent nerves in different experimental models of gastritis in rats. Functional ablation of sensory nerves was achieved by systemic capsaicin treatment (100 mg/kg). Gastritis was induced by mild (iodoacetamide, diquat, surgical duodeno-gastric reflux [DGR]) and strong (70% ethanol, indomethacin) inflammatory agents. Antagonists of the CGRP1 and NK1 receptors, hCGRP8-37 and SR140333, were administered in rats treated with iodoacetamide and ethanol. Macroscopic damage scores (MDS), myeloperoxidase (MPO) activity and malondialdehyde (MDA) concentration were evaluated after sacrifice. Macroscopic lesions appeared only in ethanol and indomethacin gastritis and were enhanced by capsaicin treatment. Gastric MPO activity was significantly increased by all agents compared to controls. Capsaicin treatment did not have any effect on MPO activity in indomethacin-treated rats or in rats submitted to surgery for duodeno-gastric reflux. However, it abolished the increase in MPO induced by iodoacetamide and diquat, and significantly enhanced that induced by ethanol. hCGRP8-37 and SR140333 abolished the increase in MPO activity and MDA concentration in iodoacetamide treated rats. In ethanol-treated rats, SR140333 diminished MPO activity. These results indicate that, depending upon the nature and duration of the experimental inflammation, capsaicin-sensitive afferent nerves may act differently to control gastric inflammatory processes, suggesting the involvement of a neurogenic component in some forms of gastric inflammation.
Larauche
Muriel
M
Neuro-Gastroenterology and Nutrition Unit, INRA, 180 chemin de Tournefeuille, BP 3, 31931 Toulouse cedex 9, France.
Anton
Pauline M
PM
Peiro
Géraldine
G
Eutamène
Hélène
H
Buéno
Lionel
L
Fioramonti
Jean
J
D000818:Animals; D015415:Biomarkers; D015740:Calcitonin Gene-Related Peptide / Q000031:analogs & derivatives / Q000378:metabolism; D000077221:Calcitonin Gene-Related Peptide Receptor Antagonists; D002211:Capsaicin / Q000494:pharmacology*; D003714:Denervation / Q000379:methods; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000294:innervation / Q000473:pathology / Q000503:physiopathology*; D005756:Gastritis / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology*; D018836:Inflammation Mediators / Q000494:pharmacology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D064729:Neurokinin-1 Receptor Antagonists; D009498:Neurotoxins / Q000494:pharmacology; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018015:Receptors, Calcitonin Gene-Related Peptide / Q000378:metabolism; D018040:Receptors, Neurokinin-1 / Q000378:metabolism; D018013:Receptors, Neuropeptide / Q000187:drug effects / Q000378:metabolism*; D011984:Sensory Receptor Cells / Q000187:drug effects / Q000378:metabolism*; D013373:Substance P / Q000031:analogs & derivatives / Q000378:metabolism; D017833:Visceral Afferents / Q000187:drug effects / Q000378:metabolism*
D015415:Biomarkers; D000077221:Calcitonin Gene-Related Peptide Receptor Antagonists; D018836:Inflammation Mediators; D064729:Neurokinin-1 Receptor Antagonists; D009498:Neurotoxins; D018015:Receptors, Calcitonin Gene-Related Peptide; D018040:Receptors, Neurokinin-1; D018013:Receptors, Neuropeptide; D013373:Substance P; D008315:Malondialdehyde; D009195:Peroxidase; D015740:Calcitonin Gene-Related Peptide; D002211:Capsaicin