title: Degenerative endometrial changes do not change the functional capacity of immigrating uterine neutrophils in mares.
abstract: An endometritis model was used to investigate the influence of degenerative endometrial changes (endometrosis) on functional parameters of uterine neutrophils in the horse. Six hours after intrauterine application of recombinant human interleukin-8 (rhIL-8), the uteri of 15 mares were flushed with phosphate-buffered saline. Quantitative and qualitative flow cytometric assays were then made to determine the absolute numbers, viability, phenotype, generation of reactive oxygen species (ROS), and phagocytic activity of immigrated polymorphonuclear neutrophilic granulocytes (PMN). Recombinant hIL-8 attracted similarly high numbers of similarly viable PMN into the uteri of mares with or without degenerative endometrial changes. Compared with blood PMN, immigrated uterine neutrophils displayed significantly upregulated expression of CD11a/CD18 (LFA-1) on uterine PMN whereas major histocompatibility complex class I molecules were expressed at lower densities. The ability to phagocytose opsonized streptococci did not differ between uterine and blood PMN. However, uterine PMN displayed a higher capacity to generate ROS. On average, uterine PMN of mares with degenerative endometrial changes showed phenotypical and functional characteristics similar to those of mares with a histologically healthy endometrium. Therefore, degenerative endometrial changes per se did not reduce the functional capacity of equine uterine neutrophils in mares.
authors.affiliation: Clinic for Cattle, School of Veterinary Medicine Hannover, Hannover, Germany. Holm.Zerbe@tiho-hannover.de
authors.forename: H
authors.initials: H
authors.lastname: Zerbe
authors.forename: F
authors.initials: F
authors.lastname: Engelke
authors.forename: E
authors.initials: E
authors.lastname: Klug
authors.forename: H-A
authors.initials: HA
authors.lastname: Schoon
authors.forename: W
authors.initials: W
authors.lastname: Leibold
mesh_terms: D000818:Animals; D004716:Endometritis / Q000473:pathology / Q000662:veterinary*; D005260:Female; D006734:Horse Diseases / Q000473:pathology*; D006736:Horses; D016130:Immunophenotyping; D016209:Interleukin-8 / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology* / Q000187:drug effects; D010587:Phagocytosis; D014599:Uterus / Q000166:cytology*
chemical_list: D016209:Interleukin-8