Multipotential electrochemical detection of primer extension reactions on DNA self-assembled monolayers.
Electroactive nucleoside triphosphates ("electrotides") have been incorporated into primers by DNA polymerase and detected on oligonucleotide surface-assembled monolayers. Four electrotides bearing three different electroactive moieties-ferrocene, vinylferrocene, and anthraquinone-are detected in four alternative formats.
Di Giusto
Daniel A
DA
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, New South Wales 2052, Australia.
Wlassoff
Wjatschesslaw A
WA
Giesebrecht
Susanne
S
Gooding
J Justin
JJ
King
Garry C
GC
D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004563:Electrochemistry / Q000379:methods*
D017931:DNA Primers; D004247:DNA; D004259:DNA-Directed DNA Polymerase