Assessment of inflammatory response and sequestration of blood iron transferrin complexes in a rat model of lung injury resulting from exposure to low-frequency shock waves.
OBJECTIVE
Impact of air blast overpressure waves (OPW), or shock wave, with the body wall or body armor produces two types of energy waves: high-frequency low-amplitude stress waves and long-duration low-frequency share waves. These types of energy waves are characterized by different mechanisms of primary tissue injury that mostly affect lung. Systemic inflammation and resultant acute respiratory distress syndrome are known major secondary causative agents of delayed multiple organ failure and subsequent death after OPW exposure. However, association of each pattern of the blast OPW-produced energy waves with postexposure inflammatory events has not yet been delineated. The objectives of the present research were a) establishment of a rat model for assessment of the inflammatory response following lung injury produced by exposure to medium-amplitude (approximately 120 kPa) low-frequency (260+/-5 Hz) OPWs; and b) assessment of the dynamics of alteration in polymorphonuclear leukocyte counts and expression of CD11b adhesion molecules on the surface of polymorphonuclear leukocytes and status of iron-transferrin complexes in peripheral blood after OPW exposure.


METHODS
This study focused on the OPW effects at different time periods, using a sequential approach to postexposure events. Lung injury in rat was induced by OPW generated in a laboratory shock tube. Animals were exposed to OPW (at peak overpressure of 118+/-7 kPa) that produced "moderate" lung injury.


METHODS
Military research institute.


METHODS
Twenty-seven CVF Sprague-Dawley rats were subjected to OPW exposures, and 17 sham-treated animals were used as control.


METHODS
Lung tissue and blood samples were collected at 1, 3, 6, 12, and 24 hrs following OPW exposures and compared with samples collected from nonexposed animals.


RESULTS
OPW-induced lung injury caused a 2.7-fold increase in the number of circulatory polymorphonuclear leukocytes as early as 1 hr postexposure, which is indicative of mobilization of the pool of marginated polymorphonuclear leukocytes into the free circulation. Polymorphonuclear leukocyte counts increased through the following 3- and 6-hr periods, when they were, respectively, 5-fold and 3.5-fold higher than in controls. These effects were accompanied by a pronounced expression of CD11b in polymorphonuclear leukocytes and tissue sequestration of blood iron-transferrin complexes during the entire 24-hr period of observations. The increase in circulatory polymorphonuclear leukocytes was accompanied by a decrease in iron-transferrin complex concentrations that apparently reflected implication of blood plasma iron in the inflammatory cell response to OPW-induced injury.


CONCLUSIONS
The observed dynamics in polymorphonuclear leukocyte alterations in peripheral blood after OPW exposure were similar to those found recently in clinical observations of nonpenetrating injury and in animal models of infectious insults. Therefore, our data suggest that the main pattern of proinflammatory alterations in the rat model of lung injury induced by exposure to long-duration shock wave is similar to patterns that are characteristic of major trauma. The data further suggest that the expression of polymorphonuclear leukocyte CD11b and the response of iron-transferrin complex can be considered as potential surrogate markers in blood for systemic alterations following OPW-induced injury and, therefore, warrant further investigation in a human pilot study.
Gorbunov
Nikolai V
NV
Department of Respiratory Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
McFaul
Steve J
SJ
Van Albert
Stephen
S
Morrissette
Craig
C
Zaucha
Gary M
GM
Nath
Jayasree
J
D000399:Air Pressure; D000818:Animals; D001753:Blast Injuries / Q000276:immunology* / Q000473:pathology; D003288:Contusions / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D019220:High-Energy Shock Waves; D007501:Iron / Q000097:blood*; D007958:Leukocyte Count; D008168:Lung / Q000276:immunology / Q000473:pathology; D055370:Lung Injury; D009504:Neutrophils / Q000276:immunology / Q000473:pathology; D051381:Rats; D012128:Respiratory Distress Syndrome / Q000276:immunology* / Q000473:pathology; D012307:Risk Factors; D018746:Systemic Inflammatory Response Syndrome / Q000276:immunology*; D014168:Transferrin / Q000378:metabolism*
D014168:Transferrin; D007501:Iron