title: Identification and sequence analysis of six new members of the NIMA-related kinase family in Chlamydomonas.
abstract: The NIMA kinases are an evolutionarily conserved protein family with enigmatic roles in the regulation of mitosis. We report six new members of this family in Chlamydomonas, in addition to the previously identified NIMA-related kinase, Fa2p. Chlamydomonas NIMA-related kinases (CNKs) 1-6 were sequenced from subclones generated by RT-PCR using information from EST libraries and the recently sequenced Chlamydomonas genome. Phylogenetic and bioinformatic approaches were used to determine the relationships of the six new members with known members of the NIMA-related kinase family. Although humans express at least eleven NIMA-related kinases, the eukaryotic microbes that have been studied to date express only one or two members of the family. Thus, the discovery that Chlamydomonas expresses a total of at least seven NIMA-related kinases is intriguing. Our analyses suggest that members of this family may play roles in the assembly and function of cilia.
authors.affiliation: Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada.
authors.forename: Brian A
authors.initials: BA
authors.lastname: Bradley
authors.forename: James J D
authors.initials: JJ
authors.lastname: Wagner
authors.forename: Lynne M
authors.initials: LM
authors.lastname: Quarmby
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000235:genetics*; D002696:Chlamydomonas / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D017124:Conserved Sequence; D017931:DNA Primers; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D000072380:NIMA-Related Kinase 1; D010802:Phylogeny; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D015800:Protozoan Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D018797:Cell Cycle Proteins; D017931:DNA Primers; D015800:Protozoan Proteins; C000606914:NEK1 protein, human; D000072380:NIMA-Related Kinase 1; D017346:Protein Serine-Threonine Kinases