[A molecular-epidemiologic study on TEM-1 genes of Neisseria gonorrhoeae in Wuxi area].
OBJECTIVE
In order to study the epidemiology of TEM-1 genes of Neisseria gonorrhoeae (Ng) in Wuxi area.


METHODS
In the light from foreign literature that positive Ng strains of beta-lactamase contain plasmid sequences of TEM-1 genes, heminested PCR for TME-1 genes of Ng detection was self-designed. Ng of 195 strains were detected, that were isolated in Wuxi area from Jan to the Oct, 2002.


RESULTS
There were 138 TEM-1 genes positive in 195 isolated strains with a positive rate of 70.8%. There was one case of heminested PCR product of TEM-1 genes which showed the homogeneity was 99% in Wuxi area, when comparing with pFA7 sequences of positive Ng plasmid of beta-lactase from register GenBank.


CONCLUSIONS
Data showed that the Ng strains with TEM-1 genes were the prevalent ones in Wuxi area.
Mi
Zu-Huang
ZH
Wuxi Clone Gen-Tech Institute, Wuxi 214026, China.
Qin
Ling
L
D002681:China / Q000453:epidemiology; D024881:Drug Resistance, Bacterial / Q000235:genetics; D006069:Gonorrhea / Q000453:epidemiology* / Q000382:microbiology; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000235:genetics*
D001618:beta-Lactamases; C034413:beta-lactamase TEM-1