Soybean bacterial artificial chromosome contigs anchored with RFLPs: insights into genome duplication and gene clustering.
Surveying the soybean genome with 683 bacterial artificial chromosome (BAC) contiguous groups (contigs) anchored by restriction fragment length polymorphisms (RFLPs) enabled us to explore microsyntenic relationships among duplicated regions and also to examine the physical organization of hypomethylated (and presumably gene-rich) genomic regions. Numerous cases where nonhomologous RFLPs hybridized to common BAC clones indicated that RFLPs were physically clustered in soybean, apparently in less than 25% of the genome. By extension, we speculate that most of the genes are clustered in less than 275 M of the soybean genome. Approximately 40%-45% of this gene-rich portion is associated with the RFLP-anchored contigs described in this study. Similarities in genome organization among BAC contigs from duplicate genomic regions were also examined. Homoeologous BAC contigs often exhibited extensive microsynteny. Furthermore, paralogs recovered from duplicate contigs shared 86%-100% sequence identity.
Mudge
Joann
J
Department of Plant Pathology, University of Minnesota, St Paul 55108, USA.
Huihuang
Yan
Y
Denny
Roxanne L
RL
Howe
Dana K
DK
Danesh
Dariush
D
Marek
Laura F
LF
Retzel
Ernie
E
Shoemaker
Randy C
RC
Young
Nevin D
ND
D022202:Chromosomes, Artificial, Bacterial; D020451:Contig Mapping; D020440:Gene Duplication; D015723:Gene Library; D023061:Gene Order; D018745:Genome, Plant; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D013025:Glycine max / Q000235:genetics*