title: Odorant stimulation enhances survival of olfactory sensory neurons via MAPK and CREB.
abstract: Olfactory sensory neurons (OSNs) can be sensitized to odorants by repeated exposure, suggesting that an animal's responsiveness to olfactory cues can be enhanced at the initial stage of detection. However, because OSNs undergo a regular cycle of apoptosis and replacement by ostensibly naive, precursor-derived neurons, the advantage of sensitization would be lost in the absence of a mechanism for odorant-enhanced survival of OSNs. Using recombinant adenoviruses in conjunction with surgical and electrophysiological techniques, we monitored OSN survival and function in vivo and find that odorant exposure selectively rescues populations of OSNs from apoptosis. We further demonstrate that odorant stimuli rescue OSNs in a cAMP-dependent manner by activating the MAPK/CREB-dependent transcriptional pathway, possibly as a result of expression of Bcl-2.
authors.affiliation: Department of Pharmacology, The University of Washington, Seattle, WA 98195, USA.
authors.forename: William C
authors.initials: WC
authors.lastname: Watt
authors.forename: Hitomi
authors.initials: H
authors.lastname: Sakano
authors.forename: Zong-Yi
authors.initials: ZY
authors.lastname: Lee
authors.forename: Jane E
authors.initials: JE
authors.lastname: Reusch
authors.forename: Kien
authors.initials: K
authors.lastname: Trinh
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Storm
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D002460:Cell Line; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism; D002470:Cell Survival / Q000235:genetics*; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D020935:MAP Kinase Signaling System / Q000502:physiology*; D008564:Membrane Potentials / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D009475:Neurons, Afferent / Q000166:cytology / Q000201:enzymology*; D009831:Olfactory Mucosa / Q000166:cytology / Q000201:enzymology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D018035:Receptors, Odorant / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012903:Smell / Q000502:physiology
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D019253:Proto-Oncogene Proteins c-bcl-2; D018035:Receptors, Odorant; D011993:Recombinant Fusion Proteins