title: Fluorescence gel retardation assay to detect protein-protein interactions.
abstract: A gel mobility retardation assay can be used to detect a protein-protein interaction. The assay is based on the electrophoretic mobility of a protein-protein complex being less than that of either protein alone. Electrophoretic mobility is detected by the fluorescence of a green fluorescent protein variant that is fused to one of the protein partners. The assay is demonstrated by using the interaction of the S-protein and S-peptide fragments of ribonuclease A as a case study.
authors.affiliation: Department of Cellular and Molecular Pharmacology, University of California-San Francisco, San Francisco, CA, USA.
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Ronald T
authors.initials: RT
authors.lastname: Raines
mesh_terms: D000818:Animals; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods*; D005453:Fluorescence; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D008164:Luminescent Proteins; D011506:Proteins; D049452:Green Fluorescent Proteins