title: D609 induces vascular endothelial cells and marrow stromal cells differentiation into neuron-like cells.
abstract: OBJECTIVE
  To investigate the effect of tricyclodecane-9-yl-xanthogenate (D609) on cell differentiation in vascular endothelial cells (VECs) and marrow stromal cells (MSCs).
  
  
  METHODS
  Morphological changes were observed under phase contrast microscope. Electron microscope and immunostaining were used for VECs identification. The expressions of neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) were examined by immunohistochemistry.
  
  
  RESULTS
  After 6 h of induction with D609, some VECs showed morphological changes characteristic of neurones. 9 h later, more VECs became neuron-like cells. About 30.8% of VECs displayed positive NSE (P<0.01), while the expression of GFAP was negative. When MSCs were exposed to D609, the cells displayed neuronal morphologies, such as pyramidal cell bodies and processes formed extensive networks at 3 h. 6 h later, almost all of the cells exhibited a typical neuronal appearance, and 85.6% of MSCs displayed intensive positive NSE, but GFAP did not express.
  
  
  CONCLUSIONS
  D609 induces VECs and MSCs differentiation into neuron-like cells.
authors.affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, Ji-nan 250100, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Chun-qing
authors.initials: CQ
authors.lastname: Du
authors.forename: Shao-shan
authors.initials: SS
authors.lastname: Wang
authors.forename: Kun
authors.initials: K
authors.lastname: Xie
authors.forename: Shang-li
authors.initials: SL
authors.lastname: Zhang
authors.forename: Jun-ying
authors.initials: JY
authors.lastname: Miao
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000166:cytology*; D001952:Bridged-Ring Compounds / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology*; D006801:Humans; D009474:Neurons / Q000166:cytology*; D009636:Norbornanes; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D017154:Stromal Cells / Q000166:cytology*; D013859:Thiocarbamates; D013871:Thiones / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D000975:Antioxidants; D001952:Bridged-Ring Compounds; D009636:Norbornanes; D010726:Phosphodiesterase Inhibitors; D013859:Thiocarbamates; D013871:Thiones; C046498:tricyclodecane-9-yl-xanthogenate