Mammalian two-hybrid assay for detecting protein-protein interactions in vivo.
Mammalian two-hybrid assay is a convenient, powerful tool to investigate protein-protein interactions in vivo. In particular, this method has a major advantage over the better known yeast version in that one can study interactions between mammalian proteins that may not fold correctly in yeast or that require post-translational modification or external stimulation that are not present in yeast.
Lee
Jae Woon
JW
Department of Medicine, Division of Diabetes, Endocrinology and Medicine, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, USA.
Lee
Soo-Kyung
SK
D000818:Animals; D002460:Cell Line; D006801:Humans; D011233:Precipitin Tests / Q000379:methods*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D014162:Transfection; D020798:Two-Hybrid System Techniques
D011506:Proteins