Two RNA polymerase complexes from vesicular stomatitis virus-infected cells that carry out transcription and replication of genome RNA.
By immunoaffinity column chromatography, we have purified two RNA polymerase complexes, the transcriptase and replicase, from vesicular stomatitis virus-infected baby hamster kidney cells. The transcriptase is a multiprotein complex, containing the virus-encoded RNA polymerase L and P proteins, and two cellular proteins, translation elongation factor-1alpha and heat-shock protein 60. In addition, the complex contains a submolar amount of cellular mRNA cap guanylyltransferase. The replicase, on the other hand, is a complex containing the viral proteins, L, P, and the nucleocapsid (N), but lacking elongation factor-1alpha, heat-shock protein 60, and guanylyltransferase. The transcriptase complex synthesizes capped mRNAs and initiates transcription at the first gene (N) start site, whereas the replicase complex initiates RNA synthesis at the precise 3' end of the genome RNA and synthesizes encapsidated replication products in the presence of the N-P complex. We propose that two RNA polymerase complexes that differ in their content of virally and host-encoded proteins are separately responsible for transcription and replication of vesicular stomatitis virus genome RNA.
Qanungo
Kaustubha R
KR
Virology Section, Department of Molecular Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA.
Shaji
Daniel
D
Mathur
Manjula
M
Banerjee
Amiya K
AK
D000818:Animals; D002460:Cell Line; D002846:Chromatography, Affinity; D006224:Cricetinae; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification / Q000502:physiology*; D016679:Genome, Viral; D012367:RNA, Viral / Q000096:biosynthesis*; D014158:Transcription, Genetic / Q000502:physiology*; D014721:Vesicular stomatitis Indiana virus / Q000201:enzymology*
D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases