[Analysis of differentially expressed lung metastasis-associated proteins in adenoid cystic carcinoma cell lines].
OBJECTIVE
To analyze differentially expressed metastasis-associated proteins in Adenoid cystic carcinoma cell lines of human salivary gland by proteomics.


METHODS
Protein expression alterations of ACC-2 and ACC-M cells were described by 2-D gels. After image analysis by software, proteins of interest were excised from the gels and identified by matrix assisted laser desorption ionization time-of-flight mass spectrometer.


RESULTS
12 protein spots showed significantly differential expression patterns between two cell lines. In the identified protein candidates, transketolase, modulator recognition factor 2, Dim1p homolog, splicing factor (arginine/serine-rich 9) and v-Ha-ras l oncogene were all lowly expressed in the poorly metastatic ACC-2 cell and significantly upregulated in highly metastatic ACC-M cell, while type I collagen pro alpha and tumor necrosis factor (ligand) superfamily member 4 showed a high expression in ACC-2 cells and a low expression in ACC-M cells. Pirin (spot 6) just appears in ACC-2 cell and was not detectable in ACC-M cell, while retinal homeobox protein was just detected in ACC-M cell and did not appear in ACC-2 cell.


CONCLUSIONS
The proteins may be involved in the adenoid cystic carcinoma lung metastasis through different mechanisms. Our work may contribute to discover diagnostic markers and therapeutic targets.
Sun
Jun-yong
JY
Department of Oral Maxillofacial Surgery, School of Stomatology, Affiliated Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China.
Guo
Wei
W
An
Jie
J
Yuan
Quan
Q
Zhao
Fu-kun
FK
D003528:Carcinoma, Adenoid Cystic / Q000556:secondary*; D002352:Carrier Proteins / Q000032:analysis; D045744:Cell Line, Tumor; D049308:Dioxygenases; D006801:Humans; D008175:Lung Neoplasms / Q000556:secondary*; D009363:Neoplasm Proteins / Q000032:analysis*; D009687:Nuclear Proteins / Q000032:analysis; D040901:Proteomics; D018631:ras Proteins / Q000032:analysis
D002352:Carrier Proteins; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D049308:Dioxygenases; C105340:PIR protein, human; D018631:ras Proteins