[Determination of contents of lipid peroxidation products in lipoproteins by using precipitation systems].
The impacts exerted by three different systems of precipitation of apo-B-containing lipoproteins, i.e. phosphotungstic acid-Mg2+ heparin-Mn2+ and heparin-Ca2+, on the lipid peroxidation (LPO) parameters in the fraction of high-density lipoproteins (HDLP) and in the aggregate fraction of low and very low density lipoproteins (LDLP, VLDLP) were comparatively analyzed. The systems of phosphotungstic acid-Mg2+ and of heparin-Ca2+ were found to have the comparable results related with the content of LDLP and VLDLP. While, the use of the precipitation system based on heparin-Mn2+ entails overstated LPO parameters in both lipoprotein fractions. It was demonstrated on the basis of all studied precipitation systems that the aggregate LDLP and VLDLP fraction had the highest content of isopropanol-soluble LPO products. The highest indices for the LPO heptane-soluble products were registered in the HDLP.
Volchegorskiĭ
I A
IA
Kharchenkova
N V
NV
D001055:Apolipoproteins B / Q000032:analysis* / Q000097:blood; D011232:Chemical Precipitation; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008074:Lipoproteins / Q000097:blood / Q000737:chemistry*; D008297:Male
D001055:Apolipoproteins B; D008074:Lipoproteins