title: Malaria blood stage parasites activate human plasmacytoid dendritic cells and murine dendritic cells through a Toll-like receptor 9-dependent pathway.
abstract: A common feature of severe Plasmodium falciparum infection is the increased systemic release of proinflammatory cytokines that contributes to the pathogenesis of malaria. Using human blood, we found that blood stage schizonts or soluble schizont extracts activated plasmacytoid dendritic cells (PDCs) to up-regulate CD86 expression and produce IFN-alpha. IFN-alpha production was also detected in malaria-infected patients, but the levels of circulating PDCs were markedly reduced, possibly because of schizont-stimulated up-regulation of CCR7, which is critical for PDC migration. The schizont-stimulated PDCs elicited a poor T cell response, but promoted gamma delta T cell proliferation and IFN-gamma production. The schizont immune stimulatory effects could be reproduced using murine DCs and required the Toll-like receptor 9 (TLR9)-MyD88 signaling pathway. Although the only known TLR9 ligand is CpG motifs in pathogen DNA, the activity of the soluble schizont extract was far greater than that of schizont DNA, and it was heat labile and precipitable with ammonium sulfate, unlike the activity of bacterial DNA. These results demonstrate that schizont extracts contain a novel and previously unknown ligand for TLR9 and suggest that the stimulatory effects of this ligand on PDCs may play a key role in immunoregulation and immunopathogenesis of human falciparum malaria.
authors.affiliation: Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand. sathitp@afrims.org
authors.forename: Sathit
authors.initials: S
authors.lastname: Pichyangkul
authors.forename: Kosol
authors.initials: K
authors.lastname: Yongvanitchit
authors.forename: Utaiwan
authors.initials: U
authors.lastname: Kum-arb
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Hemmi
authors.forename: Shizuo
authors.initials: S
authors.lastname: Akira
authors.forename: Arthur M
authors.initials: AM
authors.lastname: Krieg
authors.forename: D Gray
authors.initials: DG
authors.lastname: Heppner
authors.forename: V Ann
authors.initials: VA
authors.lastname: Stewart
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Hasegawa
authors.forename: Sornchai
authors.initials: S
authors.lastname: Looareesuwan
authors.forename: G Dennis
authors.initials: GD
authors.lastname: Shanks
authors.forename: R Scott
authors.initials: RS
authors.lastname: Miller
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics / Q000502:physiology; D002458:Cell Fractionation; D002465:Cell Movement / Q000276:immunology; D002478:Cells, Cultured; D018920:Coculture Techniques; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology* / Q000473:pathology; D006801:Humans; D007111:Immunity, Cellular; D016898:Interferon-alpha / Q000096:biosynthesis / Q000097:blood; D016778:Malaria, Falciparum / Q000097:blood / Q000276:immunology* / Q000469:parasitology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D053594:Myeloid Differentiation Factor 88; D010963:Plasmodium falciparum / Q000737:chemistry / Q000254:growth & development* / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000096:biosynthesis; D011956:Receptors, Cell Surface / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D011971:Receptors, Immunologic / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology*; D012995:Solubility; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism / Q000469:parasitology; D051217:Toll-Like Receptor 9; D015854:Up-Regulation / Q000276:immunology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000943:Antigens, Differentiation; D004268:DNA-Binding Proteins; D016898:Interferon-alpha; C507779:MYD88 protein, human; C507780:Myd88 protein, mouse; D053594:Myeloid Differentiation Factor 88; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; C495371:TLR9 protein, human; C495370:Tlr9 protein, mouse; D051217:Toll-Like Receptor 9