Analysis of estrogen-regulated genes in mouse uterus using cDNA microarray and laser capture microdissection.
The steroid hormone, estrogen, plays an important role in various physiological events which are mediated via its nuclear estrogen receptors, ERalpha and ERbeta. However, the molecular mechanisms that are regulated by estrogen in the uterus remain largely unknown. To identify genes that are regulated by estrogen, the ovariectomized mouse uterus was exposed to 17beta-estradiol (E2) for 6 h and 12 h, and the data were analyzed by cDNA microarray. The present study confirms previous findings and identifies several genes with expressions not previously known to be influenced by estrogen. These genes include small proline-rich protein 2A, receptor-activity-modifying protein 3, inhibitor of DNA binding-1, eukaryotic translation initiation factor 2, cystatin B, decorin, secreted frizzled-related protein 2, integral membrane protein 2B and chemokine ligand 12. The expression patterns of several selected genes identified by the microarray analysis were confirmed by RT-PCR. In addition, laser capture microdissection (LCM) was conducted to determine the expression of selected genes in specific uterine cell types. Analysis of early and late responsive genes using LCM and cDNA microarray not only suggests direct and indirect effects of E2 on uterine physiological events, but also demonstrates differential regulation of E2 in specific uterine cell types. These results provide a basic background on global gene alterations or genetic pathways in the uterus during the estrous cycle and the implantation period.
Hong
Seok Ho
SH
Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 133-791, Korea.
Nah
Hee Young
HY
Lee
Ji Yoon
JY
Gye
Myung Chan
MC
Kim
Chung Hoon
CH
Kim
Moon Kyoo
MK
D000818:Animals; D003627:Data Interpretation, Statistical; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation; D017781:Genes, Immediate-Early; D051379:Mice; D008813:Mice, Inbred ICR; D042282:Microdissection; D020411:Oligonucleotide Array Sequence Analysis; D010052:Ovariectomy; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014599:Uterus / Q000737:chemistry*
D004967:Estrogens