New insights in protein phosphorylation: a signature for protein phosphatase 1 interacting proteins.
Protein phosphatase 1 is regulated by the interaction between a catalytic subunit (PP1c) and multiple interacting proteins that allow the specific dephosphorylation of diverse cellular targets. This communication proposes to use the simultaneous presence of distinct consensus PP1c docking motifs R/K-x(0,1)-V-x-F and F-x-x-R/K-x-R/K as a signature to identify proteins putatively interacting with the PP1c. To develop this concept, we propose a new website, http://pp1 signature.pasteur.fr, which allows the identification of putative PP1-interacting proteins containing the two distinct PP1c docking consensus motifs represented in the Swissprot library. To validate the new concept of signature, we were able to characterise, by co-immunoprecipitation, four new PP1c interacting proteins randomly selected from the database in our website.
Garcia
Alphonse
A
Laboratoire de chimie organique, département DBSC, Institut Pasteur, 25, rue du Docteur-Roux, 75724 Paris, France. agarcia@pasteur.fr
Cayla
Xavier
X
Caudron
Bernard
B
Deveaud
Eric
E
Roncal
Fernando
F
Rebollo
Angelita
A
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D051017:Apoptosis Regulatory Proteins; D015153:Blotting, Western; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D002645:Chickens; D016384:Consensus Sequence; D030562:Databases, Protein; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology; D020407:Internet; D051379:Mice; D010455:Peptides / Q000138:chemical synthesis / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D010766:Phosphorylation; D011233:Precipitin Tests; D011485:Protein Binding; D054645:Protein Phosphatase 1; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D013601:T-Lymphocytes / Q000187:drug effects / Q000201:enzymology; D051025:bcl-Associated Death Protein; D051020:bcl-X Protein
D051017:Apoptosis Regulatory Proteins; C494819:BAD protein, human; C494811:BCL2L1 protein, human; C491073:BCL2L2 protein, human; C494820:Bad protein, mouse; C494821:Bad protein, rat; C494812:Bcl2l1 protein, mouse; C494813:Bcl2l1 protein, rat; C101155:Bcl2l2 protein, mouse; C491072:Bcl2l2 protein, rat; D002352:Carrier Proteins; D010455:Peptides; D011506:Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051025:bcl-Associated Death Protein; D051020:bcl-X Protein; D015847:Interleukin-4; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1