Binding of wogonin to human serum albumin: a common binding site of wogonin in subdomain IIA.
The binding of wogonin to human serum albumin (HSA) has been studied by spectroscopic method including circular dichroism (CD), infrared spectra (IR) and fluorescence spectra. The fluorescence properties of HSA were examined in presence of wogonin and the fluorescence intensity of HSA was significantly decreased in the presence of wogonin. The binding parameters of wogonin were studied from the fluorescence decreasing of HSA by the fluoremetric titrations. The Stern-Volmer plots indicated that the binding of wogonin to HSA at 296, 303, 310 K is characterized by one binding site with the binding constant K(S-V) at 1.872 x 10(5), 1.561 x 10(5), 1.392 x 10(5), respectively, which are good agreement with the results from the Scatchard plots. The binding process was exothermic, enthalpy driven and spontaneous, as indicated by the thermodynamic analyses, and the major part of the binding energy is hydrophobic interaction, which were consistent with the result of molecule modelling study, and there are also a numbers of hydrogen bonds between wogonin and HSA. Furthermore, the displacement experiments indicate that wogonin can bind to the subdomain IIA, that is, the site I of HSA, which is also good agreement with the result of molecule modelling study.
Tian
Jianniao
J
Department of Chemistry, Lanzhou University, Lanzhou, Gansu Province 730000, China. tianjn58@yahoo.com.cn
Liu
Jiaqin
J
Xie
Jianping
J
Yao
Xiaojun
X
Hu
Zhide
Z
Chen
Xinguo
X
D001665:Binding Sites; D002942:Circular Dichroism; D044950:Flavanones / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D008958:Models, Molecular; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics
D044950:Flavanones; D012709:Serum Albumin; C085514:wogonin