title: Nisin production by Lactococcus lactis subsp. lactis under nutritional limitation in fed-batch culture.
abstract: Nisin production was improved under nutritional limitation in fed-batch culture of Lactococcus lactis. Nisin titre reached 3887 IU ml(-1) by a slow feeding of sucrose and 4131 IU ml(-1) by slow feeding of nitrogen source, which were, respectively, 64% and 74% above the values in batch culture.
authors.affiliation: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100080, PR China.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Lv
authors.forename: Wei
authors.initials: W
authors.lastname: Cong
authors.forename: Zhaoling
authors.initials: Z
authors.lastname: Cai
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000502:physiology; D013294:Lactococcus lactis / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D009561:Nisin / Q000096:biosynthesis* / Q000302:isolation & purification; D009584:Nitrogen / Q000378:metabolism*; D009752:Nutritional Status / Q000502:physiology; D013395:Sucrose / Q000378:metabolism*
chemical_list: D009561:Nisin; D013395:Sucrose; D009584:Nitrogen