A link between transcription and intermediary metabolism: a role for Sir2 in the control of acetyl-coenzyme A synthetase.
The silent information regulator protein (Sir2) and its homologs (collectively known as sirtuins) are NAD+-dependent deacetylase enzymes involved in chromosome stability, gene silencing and cell aging in eukaryotes and archaea. The discovery that sirtuin-dependent protein deacetylation is a NAD+-consuming reaction established a link with the energy generation systems of the cell. This link to metabolism was recently extended to the post-translational control of the activity of short-chain fatty acyl-coenzyme A (adenosine monophosphate-forming) synthetases in bacteria and yeast. The crystal structure of the Sir protein complexed with a peptide of a protein substrate provided insights into how sirtuins interact with their protein substrates.
Starai
V J
VJ
Department of Bacteriology, University of Wisconsin-Madison, 264 Enzyme Institute, 1710 University Avenue, Madison, WI 53726-4087, USA.
Takahashi
H
H
Boeke
J D
JD
Escalante-Semerena
J C
JC
D000106:Acetate-CoA Ligase / Q000378:metabolism*; D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D019779:Salmonella enterica / Q000235:genetics* / Q000378:metabolism; D037761:Sirtuins / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic / Q000502:physiology
D037761:Sirtuins; D000106:Acetate-CoA Ligase