Influence of air on polybutadiene used in the preparation of stationary phases for high-performance liquid chromatography.
A 100 ml bottle of polybutadiene, PBD, was repeatedly exposed to air over a period of 6 months. Samples were taken at time zero (PBD-0), after 3 months (PBD-3) and 6 months (PBD-6). These samples were sorbed onto HPLC silica by an open-air solution-evaporation procedure, which involved exposure to the atmosphere for 6 days. Portions of the three sets of samples were used to compare self-immobilization and the effects of 100 degrees C thermal treatments in air or nitrogen on HPLC performance of the resulting phases. It is concluded that self-immobilization is enhanced by prior exposure of sorbed PBD to air and subsequent heating at 100 degrees C further enhances column performance. The best performance (10(5) plates m(-1)) resulted from 4 h heating of PBD-6 material in nitrogen.
Lopes
Nilva P
NP
LABCROM-Laboratório de Pesquisas em Cromatografia Líquida, Instituto de Química, Universidade Estadual de Campinas, Caixa Postal 6154, CEP 13084-971 Campinas, SP, Brazil.
Collins
Kenneth E
KE
Jardim
Isabel C S F
IC
D000388:Air; D002070:Butadienes / Q000737:chemistry*; D002850:Chromatography, Gel / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020724:Elastomers; D011108:Polymers / Q000737:chemistry*
D002070:Butadienes; D020724:Elastomers; D011108:Polymers; C028834:polybutadiene