title: Comparative genomic hybridization analysis of hereditary swine cutaneous melanoma revealed loss of the swine 13q36-49 chromosomal region in the nodular melanoma subtype.
abstract: Genetic alterations implicated in malignant melanoma are still poorly understood. Malignant melanomas present highly variable histologic and cytologic patterns. The aim of the present study is to define genomic imbalances associated with the development of 2 histologic types of swine hereditary cutaneous melanoma. We have investigated 11 swine tumors by comparative genomic hybridization (CGH), 4 superficial spreading melanomas (SSMs) and 7 nodular melanomas (NMs). Following laser capture microdissection and degenerate oligonucleotide primed-polymerase chain reaction, we were able to isolate and then amplify DNA from the 2 histologic subtypes. Consensus regions of chromosome gains were identified on both histologic subtypes, on swine chromosomes 3p13-p17 (75% of the SSMs and 71% of the NMs), 12q (100% of the SSMs and 57% of the NMs) and 14q11-q21 (75% of the SSMs and 42% of NMs). Chromosomal loss was restricted to NM lesions and the swine 13q36-49 region was lost in 100% of the NMs. Interphase fluorescence in situ hybridization with a probe mapping to the 13q41-q42 region indicates loss of the corresponding region on NM lesions. Taking into account this CGH analysis and the comparative genomic data between swine and human genomes, we suggest that a role for the human chromosomes 3p11-qter and chromosome 21 losses should be investigated in human nodular melanoma progression.
authors.affiliation: Institut Curie-Recherche, Paris, France. francoise.apiou@curie.fr
authors.forename: Françoise
authors.initials: F
authors.lastname: Apiou
authors.forename: Silvia
authors.initials: S
authors.lastname: Vincent-Naulleau
authors.forename: Alain
authors.initials: A
authors.lastname: Spatz
authors.forename: Philippe
authors.initials: P
authors.lastname: Vielh
authors.forename: Claudine
authors.initials: C
authors.lastname: Geffrotin
authors.forename: Geffrotin
authors.initials: G
authors.lastname: Frelat
authors.forename: Bernard
authors.initials: B
authors.lastname: Dutrillaux
authors.forename: Catherine
authors.initials: C
authors.lastname: Le Chalony
mesh_terms: D000818:Animals; D002872:Chromosome Deletion; D002882:Chromosomes, Human, Pair 13; D002893:Chromosomes, Human, Pair 3; D005260:Female; D006801:Humans; D008297:Male; D008545:Melanoma / Q000235:genetics* / Q000473:pathology; D009693:Nucleic Acid Hybridization; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology; D013552:Swine