Chromatography paper strip method for collection, transportation, and storage of rotavirus RNA in stool samples.
We developed a novel method that uses sodium dodecyl sulfate-EDTA-treated chromatography paper strips to collect unconcentrated fresh stool samples. After the paper strips were stored for 4 months at room temperature, rotavirus RNA could be successfully amplified by using reverse transcriptase PCR. The use of filter paper strips as a specimen support allows (self-)collection of stool samples by untrained persons. Diarrheal stool samples from remote areas can be stored and transported to a central diagnostic laboratory without the need for freezers or special shipping conditions. This convenient and inexpensive rotavirus sample collection system can be of use in epidemiological surveillance studies and vaccine trials.
Rahman
Mustafizur
M
Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, University of Leuven, Belgium.
Goegebuer
Truus
T
De Leener
Karolien
K
Maes
Piet
P
Matthijnssens
Jelle
J
Podder
Goutam
G
Azim
Tasnim
T
Van Ranst
Marc
M
D002854:Chromatography, Paper; D003967:Diarrhea / Q000821:virology; D004492:Edetic Acid; D005243:Feces / Q000821:virology*; D006801:Humans; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D011934:Reagent Strips; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000821:virology; D012967:Sodium Dodecyl Sulfate; D013048:Specimen Handling / Q000379:methods*
D012367:RNA, Viral; D011934:Reagent Strips; D012967:Sodium Dodecyl Sulfate; D004492:Edetic Acid