Growth and sporulation of Bacillus cereus ATCC 14579 under defined conditions: temporal expression of genes for key sigma factors.
An airlift fermentor system allowing precise regulation of pH and aeration combined with a chemically defined medium was used to study growth and sporulation of Bacillus cereus ATCC 14579. Sporulation was complete and synchronous. Expression of sigA, sigB, sigF, and sigG was monitored with real-time reverse transcription-PCR, and the pattern qualitatively resembled that of Bacillus subtilis. This method allows reproducible production of stable spores, while the synchronous growth and defined conditions are excellently suitable for further gene expression studies of cellular differentiation of B. cereus.
de Vries
Ynte P
YP
Wageningen Centre for Food Sciences, Laboratory of Food Microbiology, Wageningen UR, The Netherlands. Ynte.deVries@wur.nl
Hornstra
Luc M
LM
de Vos
Willem M
WM
Abee
Tjakko
T
D001409:Bacillus cereus / Q000166:cytology / Q000235:genetics / Q000254:growth & development* / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002454:Cell Differentiation; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D007700:Kinetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012808:Sigma Factor / Q000235:genetics; D013171:Spores, Bacterial / Q000502:physiology
D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; C064971:FliA protein, Bacteria; C079011:SigB protein, Bacteria; D012808:Sigma Factor