Fluorometric assay protocol for protease-catalyzed transesterification reactions in organic solvents.
A flourometric assay protocol for a subtilisin-catalyzed transesterification reaction in n-hexane has been developed. The method makes use of a Michael acceptor that forms a fluorescent adduct with thiophenol, one of the products generated in the transesterification reaction. The method may be employed for screening a biocatalyst useful for transesterification reactions in organic solvents and for optimizing the transesterification reaction conditions.
Han
Min Su
MS
Center for Integrated Molecular Systems, and National Research Laboratory of Chirotechnology, Department of Chemistry, Pohang University of Science and Technology, San 31 Hyojadong, Pohang 790-784, Korea.
Jung
Sang Oh
SO
Kim
Mahn-Joo
MJ
Kim
Dong H
DH