title: Separation of free iodide from other I-species in human serum - Quantification in serum pools and individual samples.
abstract: A method for the determination of free iodide in human serum has been developed. Iodide from pooled serum samples has been separated from the organic matter by SEC, subsequently freeze-dried and analyzed by ion chromatography. Investigations for recovery and precision have been carried out and provided sufficient results. For quality assurance ICP-MS has been taken additionally as a total I-detector. The iodide results of ICP-MS agree well with IC values. Iodine containing SEC- fractions from iodide-spiked samples has shown no increased I-values except that in the iodide fractions, proving that there has been no iodide conversion into other I-species (and vice versa) during the whole procedure. Free iodide from two serum pools of different healthy persons has been determined as 2.25 and 2.43 microg I(-)/l, respectively. The values are related to total iodine levels determined by ICP-MS. For comparative reasons a table of individual iodine and iodide values is presented.
authors.affiliation: GSF-Forschungszentrum für Umwelt und Gesundheit, Institut für Okologische Chemie, Neuherberg, D-85764, Oberschleissheim, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Michalke
authors.forename: P
authors.initials: P
authors.lastname: Schramel
authors.forename: S
authors.initials: S
authors.lastname: Hasse