Altered gene expressions in rat hippocampus after kainate injection with or without melatonin pre-treatment.
The expressions of Bcl-2, Bax and thioredoxin (Trx) mRNAs after kainic acid (KA) injection with or without melatonin pre-treatment were examined by real-time quantitative reverse transcription polymerase chain reaction in rat hippocampus. Bcl-2, Bax, and Trx mRNA expressions after KA injection were significantly increased. Additionally, it was observed that melatonin or melatonin pre-treatment had no significant effect on the regulation of Trx mRNA. Pre-treatment with melatonin at the 30th minute before KA injection resulted in a significant depletion in Bcl-2, Bax and Trx mRNA expressions. However, our results showed that melatonin pre-treatment increases the ratio of Bcl-2 to Bax mRNA in short-term period.
Yalcin
Ayfer
A
Department of Biochemistry, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey. yalcina@pharm.ege.edu.tr
Kanit
Lütfiye
L
Sozmen
Eser Y
EY
D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000502:physiology; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D007608:Kainic Acid / Q000494:pharmacology*; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D008550:Melatonin; D007608:Kainic Acid