title: [Combination of adenovirus p16(INK4A) gene therapy and ionizing radiation for laryngeal squamous cell carcinoma].
abstract: OBJECTIVE
  To assess the anti-tumor effect of p16(INK4A) gene transfer and the combined effect of this gene therapy and ionizing radiation in the treatment of laryngeal squamous cell carcinoma.
  
  
  METHODS
  A Complete p16(INK4A) gene was inserted into a replication-defective recombinant adenovirus, and the tumor cells were infected with Adenovinus-p16 (Ad-p16) and irradiated with X-rays. Confirmation of P16(INK4A) protein expression after Ad-p16 infection was performed by Western blotting. The therapeutic effects were evaluated both in vitro and in vivo.
  
  
  RESULTS
  The replication-defective recombinant adenovirus could direct a high level of P16INK4A protein expression in laryngeal squamous cell carcinoma. Studies both in vitro and in vivo demonstrated that Ad-p16 treatment significantly inhibited the cell growth and the established tumors in mude mice when compared with the control treatments (P < 0.05). The combination of Ad-p16 and radiation group resulted in greater inhibition of tumor growth, compared with any other treatment groups and controls (P < 0.05). No significant difference was observed between Adenovirus-LacZ (Ad-LacZ) and PBS groups.
  
  
  CONCLUSIONS
  These results demonstrate a significant antitumor effect of Ad-p16 against human laryngeal squamous cell carcinoma and also demonstrate for the first time a combined effect of radiation and Ad-p16 gene transfer in p16 deficient human laryngeal squamous cell carcinoma.
authors.affiliation: Department of Otolaryngology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Yan-jun
authors.initials: YJ
authors.lastname: Fu
authors.forename: Shi-xi
authors.initials: SX
authors.lastname: Liu
authors.forename: Jun-ming
authors.initials: JM
authors.lastname: Xian
mesh_terms: D000256:Adenoviridae; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000532:radiotherapy / Q000628:therapy*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics* / Q000378:metabolism; D018014:Gene Transfer Techniques; D019942:Genes, p16; D015316:Genetic Therapy / Q000379:methods*; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000532:radiotherapy / Q000628:therapy*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D018714:Radiotherapy, Adjuvant; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16