Determination of cefuroxime in human serum or plasma by liquid chromatography with electrospray tandem mass spectrometry.
Cefuroxime is a second-generation cephalosporin used against different kinds of bacterial infections. To be able to optimize the dosing it is necessary to characterize the pharmacokinetics of cefuroxime which requires a selective and sensitive analytical method for cefuroxime in plasma or serum. A new rapid liquid chromatography/electrospray tandem mass spectrometry (LC/MS/MS) method, using cefotaxime as internal standard, was developed for analysis of cefuroxime in human serum. The work-up procedure consisted of protein precipitation with acetonitrile/cefotaxime, and after centrifugation the supernatant was dissolved in mobile phase. The sample was injected on a SB-CN column and the detection was performed using tandem mass spectrometry (MS/MS). The limit of quantification was determined to 0.025 microg/mL. The method was linear in the range 0.025-50 microg/mL with a coefficient of correlation >0.999. The limit of quantification and intra-day variability were found to be the same for plasma samples, which indicates that the method is valid for serum as well as plasma samples.
Viberg
Anders
A
Division of Pharmacokinetics and Drug Therapy, Department of Biopharmaceutical Sciences, Uppsala University, BMC, Box 591, SE-751 24 Uppsala, Sweden. Anders.Viberg@farmbio.uu.se
Sandström
Marie
M
Jansson
Britt
B
D000900:Anti-Bacterial Agents / Q000097:blood* / Q000737:chemistry; D002439:Cefotaxime / Q000097:blood; D002444:Cefuroxime / Q000097:blood* / Q000737:chemistry; D002853:Chromatography, Liquid; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000900:Anti-Bacterial Agents; D002439:Cefotaxime; D002444:Cefuroxime