Production of xylitol from Candida tropicalis by using an oxidation-reduction potential-stat controlled fermentation.
An on-line device, ORP (oxidation-reduction potential)-stat, was used to control glucose-feeding for enhancing xylitol conversion from D-xylose during an oxygen-limited fermentation by Candida tropicalis. The fermentation was carried out in a 5 l jar fermenter. After glucose in the medium was depleted, a switching to a limited aeration and feeding glucose controlled by ORP-stat was performed. The maximum xylitol yield was obtained under a condition at an ORP of - 180 mV and at an aeration rate of 0.2 l min(-1).
Sheu
Dey-Chyi
DC
Department of Bioengineering, Tatung University, Taipei 104, Taiwan. dcsheu@ttu.edu.tw
Duan
Kow-Jen
KJ
Jou
Shiuan-Ru
SR
Chen
Ying-Chou
YC
Chen
C Will
CW
D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D041022:Candida tropicalis / Q000378:metabolism*; D002241:Carbohydrates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D008956:Models, Chemical; D010084:Oxidation-Reduction; D013997:Time Factors; D014993:Xylitol / Q000737:chemistry / Q000378:metabolism*; D014994:Xylose / Q000737:chemistry
D002241:Carbohydrates; D014994:Xylose; D005947:Glucose; D014993:Xylitol