[Gene expression profiles and effects of transforming growth factor-beta1 intervention in Peyronie's disease].
OBJECTIVE
To demonstrate molecular insight into the pathology of Peyronie's disease (PD). A preliminary profile of differential gene expression between the PD plaque and control tunica albuginea was obtained with DNA microarrays. Also, to investigate the effect of intervention in PD cells, transforming growth factor-beta1 (TGF-beta1) was recruited to treat PD cell lines.


METHODS
Three PD plaques and control tunica albugineas were constructed and studied. cDNA probes were prepared from RNA isolated from those cells and hybridized with the Clontech Atlas 3.6 Array. Relative changes of greater than 2.0 defined up-regulation and down-regulation, respectively. The expression of selected individual gene MCP-1 and the effect of TGF-beta1 on MCP-1 were analyzed by reverse transcriptase-polymerase chain reaction.


RESULTS
Some up-regulated genes in the PD plaque detected by the Clontech assay were screened, one of them was monocyte chemotactic protein. One involved the pathogenesis of PD as a downstream gene and responded to the TGF-beta1 treatment but not CTGF. The results were also confirmed by TR-PCR in all the types of cell.


CONCLUSIONS
The cell lines from plaque tissue and normal tunica from men with PD were successfully established. The findings indicate a potential role for MCP-1 over expression in the pathogenesis of PD as a downstream gene regulated by some genes and could be a new therapeutic target in PD. The information may allow a better understanding of the basic mechanisms involved in the etiology and pathogenesis of PD. Furthermore, it may permit some strategies of therapeutic interventions combine routine methods with Chinese herbal medicine.
Wang
Zhong
Z
Department of Urology, Huashan Hospital, Fudan University, Shanghai 200040, China.
Liu
Jing-fang
JF
Zhou
Zhi-heng
ZH
Zhang
Yuan-fang
YF
Wang
Wen-jian
WJ
Zhang
Peng
P
Selcuk
Yucel
Y
Guiting
Lin
L
Lin
Ching-shwun
CS
D002460:Cell Line; D018932:Chemokine CCL2; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D010411:Penile Induration / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D011506:Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
C111835:CCL2 protein, human; D018932:Chemokine CCL2; D011506:Proteins; D016212:Transforming Growth Factor beta