title: Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27.
abstract: The anaphase-promoting complex (APC/C) is a large ubiquitin-protein ligase which controls progression through anaphase by triggering the degradation of cell cycle regulators such as securin and B-type cyclins. The APC/C is an unusually complex ligase containing at least 10 different, evolutionarily conserved components. In contrast to APC/C's role in cell cycle regulation little is known about the functions of individual subunits and how they might interact with each other. Here, we have analyzed Swm1/Apc13, a small subunit recently identified in the budding yeast complex. Database searches revealed proteins related to Swm1/Apc13 in various organisms including humans. Both the human and the fission yeast homologues are associated with APC/C subunits, and they complement the phenotype of an SWM1 deletion mutant of budding yeast. Swm1/Apc13 promotes the stable association with the APC/C of the essential subunits Cdc16 and Cdc27. Accordingly, Swm1/Apc13 is required for ubiquitin ligase activity in vitro and for the timely execution of APC/C-dependent cell cycle events in vivo.
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics. Scionics Computer Innovation GmbH, Dresden, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Schwickart
authors.forename: Jan
authors.initials: J
authors.lastname: Havlis
authors.forename: Bianca
authors.initials: B
authors.lastname: Habermann
authors.forename: Aliona
authors.initials: A
authors.lastname: Bogdanova
authors.forename: Alain
authors.initials: A
authors.lastname: Camasses
authors.forename: Tobias
authors.initials: T
authors.lastname: Oelschlaegel
authors.forename: Andrej
authors.initials: A
authors.lastname: Shevchenko
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Zachariae
mesh_terms: D000595:Amino Acid Sequence; D064173:Anaphase-Promoting Complex-Cyclosome; D000818:Animals; D064191:Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome; D002453:Cell Cycle / Q000502:physiology; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002842:Chromatids / Q000378:metabolism; D004258:DNA Polymerase III; D019143:Evolution, Molecular; D005816:Genetic Complementation Test; D006801:Humans; D008540:Meiosis / Q000502:physiology; D008969:Molecular Sequence Data; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins; D016415:Sequence Alignment; D043743:Ubiquitin-Protein Ligase Complexes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases
chemical_list: D064191:Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome; C577355:CDC27 protein, S cerevisiae; C577366:CDC27 protein, human; D018797:Cell Cycle Proteins; D021122:Protein Subunits; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C513024:cdc27 protein, S pombe; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome; D044767:Ubiquitin-Protein Ligases; D004258:DNA Polymerase III; C577465:SWM1 protein, S cerevisiae