Biofilm formation by Salmonella spp. and Listeria monocytogenes on plastic surface.
OBJECTIVE
To investigate the biofilm formation by 122 Salmonella spp. and 48 Listeria monocytogenes strains on a plastic surface.


METHODS
Quantification of biofilm formation was performed in brain heart infusion (BHI), trypcase soya broth (TSB), meat broth (MB) and 1/20 diluted trypcase soya broth (1/20-TSB) in plastic microtitre plates.


RESULTS
All tested Salmonella spp. and L. monocytogenes strains produced biofilm in a suitable medium. However, the quantities of biofilm produced by Salmonella spp. were greater than those produced by tested L. monocytogenes strains. The nutrient content of the medium significantly influenced the quantity of produced biofilm. Diluted TSB was the most effective in promoting biofilm production by Salmonella spp., followed by TSB, while the least quantity of biofilm was formed in BHI and MB. L. monocytogenes produced the highest quantities of biofilm in BHI, followed by TSA, then MB, and the least quantities of biofilm were produced in 1/20-TSB.


CONCLUSIONS
Salmonella spp. produces more biofilm in nutrient-poor medium, while L. monocytogenes produce more biofilm in nutrient-rich medium.
Stepanović
S
S
Institute of Microbiology and Immunology, School of Medicine, Belgrade, Serbia.
Cirković
I
I
Ranin
L
L
Svabić-Vlahović
M
M
D018441:Biofilms / Q000254:growth & development*; D003470:Culture Media; D008089:Listeria monocytogenes / Q000254:growth & development*; D010969:Plastics; D012475:Salmonella / Q000145:classification / Q000254:growth & development*; D013499:Surface Properties
D003470:Culture Media; D010969:Plastics