title: Peroxisome proliferator-activated receptor gamma is required in mature white and brown adipocytes for their survival in the mouse.
abstract: The peroxisome proliferator-activated receptor gamma (PPARgamma) mediates the activity of the insulin-sensitizing thiazolidinediones and plays an important role in adipocyte differentiation and fat accretion. The analysis of PPARgamma functions in mature adipocytes is precluded by lethality of PPARgamma(-/-) fetuses and tetraploid-rescued pups. Therefore we have selectively ablated PPARgamma in adipocytes of adult mice by using the tamoxifen-dependent Cre-ER(T2) recombination system. We show that mature PPARgamma-null white and brown adipocytes die within a few days and are replaced by newly formed PPARgamma-positive adipocytes, demonstrating that PPARgamma is essential for the in vivo survival of mature adipocytes, in addition to its well established requirement for their differentiation. Our data suggest that potent PPARgamma antagonists could be used to acutely reduce obesity.
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, Université Louis Pasteur, BP10142, 1 Rue Laurent Fries, 67404 Illkirch, France.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Imai
authors.forename: Reiko
authors.initials: R
authors.lastname: Takakuwa
authors.forename: Sandra
authors.initials: S
authors.lastname: Marchand
authors.forename: Emilie
authors.initials: E
authors.lastname: Dentz
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Bornert
authors.forename: Nadia
authors.initials: N
authors.lastname: Messaddeq
authors.forename: Olivia
authors.initials: O
authors.lastname: Wendling
authors.forename: Manuel
authors.initials: M
authors.lastname: Mark
authors.forename: Béatrice
authors.initials: B
authors.lastname: Desvergne
authors.forename: Walter
authors.initials: W
authors.lastname: Wahli
authors.forename: Pierre
authors.initials: P
authors.lastname: Chambon
authors.forename: Daniel
authors.initials: D
authors.lastname: Metzger
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000502:physiology; D000273:Adipose Tissue / Q000166:cytology* / Q000502:physiology; D002001:Adipose Tissue, Brown / Q000166:cytology* / Q000502:physiology; D000818:Animals; D001483:Base Sequence; D002470:Cell Survival; D017931:DNA Primers; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D016133:Polymerase Chain Reaction; D018160:Receptors, Cytoplasmic and Nuclear / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D017384:Sequence Deletion; D014157:Transcription Factors / Q000172:deficiency / Q000235:genetics / Q000502:physiology*
chemical_list: D017931:DNA Primers; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors