title: Effect of electro-acupuncture stimulation of different frequencies and intensities on ovarian blood flow in anaesthetized rats with steroid-induced polycystic ovaries.
abstract: BACKGROUND
  Maintenance of ovarian blood flow (OBF) is suggested to be important for regular ovulation in women with polycystic ovaries (PCO). The purpose of the present study was to investigate whether electro-acupuncture (EA) of different frequencies and intensities can improve the OBF of anaesthetized rat in the animal model of PCO.
  
  
  METHODS
  PCO was experimentally induced by a single intramuscular (i.m.) injection of estradiol valerate (EV) in rats. Control rats were given i.m. injection of oil. The involvement of the two ovarian sympathetic nerves; superior ovarian nerve (SON) and plexus ovarian nerve (OPN), in OBF responses was elucidated by severance of SON and OPN in both control and PCO rats. How systemic circulatory changes affect OBF was evaluated by continuous recording of the blood pressure. OBF was measured on the surface of the ovary-using laser Doppler flowmetry. Acupuncture needles were inserted bilaterally into the abdominal and hind limb muscles and connected to an electrical stimulator. Two frequencies--2 Hz (low) and 80 Hz (high)--with three different intensities--1.5, 3, and 6 mA--were applied for 35 s.
  
  
  RESULTS
  Low-frequency EA at intensities of 3 and 6 mA elicited significant increases in OBF in the Control group compared to baseline. In the PCO group the increases in OBF were significant only when stimulating with low-frequency EA at 6 mA. After severance of the ovarian sympathetic nerves, the increased response of OBF that had been induced by low-frequency EA in both the Control and PCO group was abolished, indicating that the OBF response is mediated via the ovarian sympathetic nerves. High-frequency EA at 6 mA significantly decreased OBF and mean arterial blood pressure (MAP) in the Control group compared to baseline. In the PCO group, the same stimulation produced similar decreases in MAP, but not in OBF.
  
  
  CONCLUSIONS
  Low-frequency EA stimulation with a strong intensity (6 mA) increases OBF in rats with steroid-induced PCO whereas less strong intensity (3 mA) induces similar changes in control rats. Severance of the ovarian sympathetic nerves, abolish this OBF increase in both study groups, which suggests that the responses of OBF to EA are mediated via the ovarian sympathetic nerves.
authors.affiliation: Department of Physiology, Sahlgrenska Academy, Göteborg University, Box 432, SE-405 30 Göteborg, Sweden. elsv@fhs.gu.se
authors.forename: Elisabet
authors.initials: E
authors.lastname: Stener-Victorin
authors.forename: Rie
authors.initials: R
authors.lastname: Kobayashi
authors.forename: Orie
authors.initials: O
authors.lastname: Watanabe
authors.forename: Thomas
authors.initials: T
authors.lastname: Lundeberg
authors.forename: Mieko
authors.initials: M
authors.lastname: Kurosawa
mesh_terms: D000758:Anesthesia; D000818:Animals; D001794:Blood Pressure; D004195:Disease Models, Animal; D015671:Electroacupuncture; D005260:Female; D017078:Laser-Doppler Flowmetry; D010053:Ovary / Q000098:blood supply* / Q000294:innervation; D011085:Polycystic Ovary Syndrome / Q000139:chemically induced* / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow / Q000502:physiology*; D013256:Steroids / Q000494:pharmacology*; D013562:Sympathectomy
chemical_list: D013256:Steroids