title: [The expression and possible function of RhoA in human gastric cancer cell lines].
abstract: OBJECTIVE
  To study the expression and possible function of RhoA in human gastric cancer cell lines.
  
  
  METHODS
  The expression of RhoA in human gastrointestinal cancer cell lines was detected by Western blot. Antisense plasmid of RhoA was constructed by pGEFL and transferred into gastric cancer cell line AGS by lipofectamine. Cell survival was examined by MTT assays, and cell cycle was detected by flow cytometry.
  
  
  RESULTS
  The expression of RhoA protein in 10 different kinds of human cancer cell lines was much higher than that in immortalized human intestinal epithelial cell line. After being transfected with antisense RhoA, with the decrease in RhoA protein expression, the growth rate of AGS was inhibited, and the number of cells in S phase was increased by 14%.
  
  
  CONCLUSIONS
  RhoA is overexpressed in many human cancer cell lines. Some of the malignant characteristics of a gastric cancer cell line can be partially reversed by inhibiting RhoA expression.
authors.affiliation: Institute of Digestive Diseases, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Bi
authors.forename: Yang-lin
authors.initials: YL
authors.lastname: Pan
authors.forename: Yan
authors.initials: Y
authors.lastname: Xue
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
authors.forename: Yong-quan
authors.initials: YQ
authors.lastname: Shi
authors.forename: Yu-mei
authors.initials: YM
authors.lastname: Zhang
authors.forename: Jing-ping
authors.initials: JP
authors.lastname: Du
authors.forename: Dai-ming
authors.initials: DM
authors.lastname: Fan
mesh_terms: D016375:Antisense Elements (Genetics) / Q000494:pharmacology; D002453:Cell Cycle; D045744:Cell Line, Tumor; D015316:Genetic Therapy; D006801:Humans; D013274:Stomach Neoplasms / Q000737:chemistry* / Q000473:pathology / Q000628:therapy; D020742:rhoA GTP-Binding Protein / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000502:physiology*
chemical_list: D016375:Antisense Elements (Genetics); D020742:rhoA GTP-Binding Protein