title: Identification of hydroxy fatty acids by liquid chromatography-atmospheric pressure chemical ionization mass spectroscopy in Euglena gracilis.
abstract: Hydroxy fatty acids from Euglena gracilis were identified by reverse-phase high performance liquid chromatography coupled to a mass spectrometer run in atmospheric pressure chemical ionization positive ion mode. These metabolites were converted to methyl esters to improve stability and chromatographic properties. A detection limit of 20 pg/microl per injection was determined for 5-HETE methyl ester based on the signal to noise ratio of the m/z 317 ion which corresponds to the loss of a hydroxyl group (M-17) and the major fragment in all HETE methyl esters studied. This is the first report for these metabolites in E. gracilis.
authors.affiliation: Department of Ecology, Evolution and Marine Biology, University of California, Santa Barbara, CA 93106, USA.
authors.forename: Lory Z
authors.initials: LZ
authors.lastname: Santiago-Vázquez
authors.forename: Laura D
authors.initials: LD
authors.lastname: Mydlarz
authors.forename: James G
authors.initials: JG
authors.lastname: Pavlovich
authors.forename: Robert S
authors.initials: RS
authors.lastname: Jacobs
mesh_terms: D000818:Animals; D001274:Atmospheric Pressure; D002853:Chromatography, Liquid / Q000379:methods*; D005056:Euglena gracilis / Q000737:chemistry*; D005227:Fatty Acids / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D005227:Fatty Acids