Primary structure of water buffalo alpha-lactalbumin variants A and B.
A novel electrophoretic alpha-lactalbumin (alpha-la) variant was detected in the Italian water buffalo breed. The isoelectric point of the variant, labelled A, was lower than the most frequent variant B. It presented an allelic frequency of 0.5% compared with the 97.1% of the BB allele. From Liquid Chromatography-Electrospray Ionization/Mass spectrometry, the molecular mass of the two alpha-la A and B variants were measured as 14,235.1+/-0.8 and 14,236.1+/-0.9 Da, respectively. The two proteins were sequenced and differentiated from one another by a single amino acid substitution, Asn45(B)-->Asp45(A). As this amino acid substitution altered the N-glycosylation sequence consensus Asn45-X-Ser46 it may be deduced that the protein glycosylation level of the alpha-la A would decrease.
Chianese
Lina
L
Dipartimento di Scienza degli Alimenti, Università degli Studi di Napoli Federico II, Portici, Italy. chianese@unina.it
Caira
Simonetta
S
Lilla
Sergio
S
Pizzolongo
Fabiana
F
Ferranti
Pasquale
P
Pugliano
Giovanni
G
Addeo
Francesco
F
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D002020:Buffaloes; D002851:Chromatography, High Pressure Liquid; D005787:Gene Frequency; D014644:Genetic Variation; D006031:Glycosylation; D015151:Immunoblotting; D007525:Isoelectric Focusing; D007768:Lactalbumin / Q000737:chemistry* / Q000235:genetics; D008894:Milk Proteins / Q000737:chemistry; D008969:Molecular Sequence Data; D021241:Spectrometry, Mass, Electrospray Ionization; D000067816:Whey Proteins
D008894:Milk Proteins; D000067816:Whey Proteins; D007768:Lactalbumin