Synovectomy reduces stromal-cell-derived factor-1 (SDF-1) which is involved in the destruction of cartilage in osteoarthritis and rheumatoid arthritis.
We have compared the concentrations of stromal-cell-derived factor-1 (SDF-1), matrix metalloproteinase-1 (MMP-1), MMP-9 and MMP-13 in serum before and after synovectomy or total knee replacement (TKR). We confirmed the presence of SDF-1 and its receptor CXCR4 in the synovium and articular cartilage by immunohistochemistry. We established chondrocytes by using mutant CXCR4 to block the release of MMPs. The level of SDF-1 was decreased 5.1- and 6.7-fold in the serum of patients with OA and RA respectively, after synovectomy compared with that before surgery. MMP-9 and MMP-13 were decreased in patients with OA and RA after synovectomy. We detected SDF-1 in the synovium and the bone marrow but not in cartilage. CXCR4 was detected in articular cartilage. SDF-1 increased the release of MMP-9 and MMP-13 from chondrocytes in a dose-dependent manner. The mutant CXCR4 blocked the release of MMP-9 and MMP-13 from chondrocytes by retrovirus vector. Synovectomy is effective in patients with OA or RA because SDF-1, which can regulate the release of MMP-9 and MMP-13 from articular chondrocytes for breakdown of cartilage, is removed by the operation.
Kanbe
K
K
Department of Orthopaedic Surgery, Tokyo Women's Medical University, Daini Hospital, Japan.
Takemura
T
T
Takeuchi
K
K
Chen
Q
Q
Takagishi
K
K
Inoue
K
K
D000368:Aged; D000369:Aged, 80 and over; D001172:Arthritis, Rheumatoid / Q000097:blood* / Q000601:surgery; D001853:Bone Marrow / Q000737:chemistry; D002357:Cartilage Diseases / Q000097:blood*; D002358:Cartilage, Articular / Q000737:chemistry; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000378:metabolism*; D019902:Chondrocytes / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000097:blood* / Q000601:surgery; D019718:Receptors, CXCR4 / Q000378:metabolism; D044967:Serum; D000074402:Synovectomy; D013583:Synovial Membrane / Q000737:chemistry
C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D019718:Receptors, CXCR4; D020782:Matrix Metalloproteinases