[Increase in the level of proline and osmotic pressure of cytoplasm in transformed tobacco bearing an antisense suppressor of the proline dehydrogenase gene].
The antisense suppressor was constructed for proline dehydrogenase gene (PDH; a fragment of PDH from Arabidopsis in antisense orientation and under the control of 35S promoter of cauliflower mosaic virus, CMV). In Nicotiana tabacum SR1 tobacco transformants bearing antisense suppressor for PDH, the proline content and the cytoplasm osmotic pressure were increased. The proline content in these transformants varied, whereas cytoplasm osmotic pressure was stable, which seems to reflect complicated relationships between these characteristics of the plant cell.
Kochetov
A V
AV
Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, 630090 Russia.
Titov
S E
SE
Kolodiazhnaia
Ia S
IaS
Komarova
M
M
Koval'
V S
VS
Makarova
N N
NN
Ilinskiĭ
Iu Iu
IuIu
Trifonova
E A
EA
Shumnyĭ
V K
VK
D017796:Caulimovirus / Q000235:genetics; D003593:Cytoplasm / Q000378:metabolism*; D009997:Osmotic Pressure; D011392:Proline / Q000378:metabolism*; D011394:Proline Oxidase / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D014170:Transformation, Genetic
D011392:Proline; D011394:Proline Oxidase