title: [A molecular-epidemiologic study on TEM-1 genes of Neisseria gonorrhoeae in Wuxi area].
abstract: OBJECTIVE
  In order to study the epidemiology of TEM-1 genes of Neisseria gonorrhoeae (Ng) in Wuxi area.
  
  
  METHODS
  In the light from foreign literature that positive Ng strains of beta-lactamase contain plasmid sequences of TEM-1 genes, heminested PCR for TME-1 genes of Ng detection was self-designed. Ng of 195 strains were detected, that were isolated in Wuxi area from Jan to the Oct, 2002.
  
  
  RESULTS
  There were 138 TEM-1 genes positive in 195 isolated strains with a positive rate of 70.8%. There was one case of heminested PCR product of TEM-1 genes which showed the homogeneity was 99% in Wuxi area, when comparing with pFA7 sequences of positive Ng plasmid of beta-lactase from register GenBank.
  
  
  CONCLUSIONS
  Data showed that the Ng strains with TEM-1 genes were the prevalent ones in Wuxi area.
authors.affiliation: Wuxi Clone Gen-Tech Institute, Wuxi 214026, China.
authors.forename: Zu-Huang
authors.initials: ZH
authors.lastname: Mi
authors.forename: Ling
authors.initials: L
authors.lastname: Qin
mesh_terms: D002681:China / Q000453:epidemiology; D024881:Drug Resistance, Bacterial / Q000235:genetics; D006069:Gonorrhea / Q000453:epidemiology* / Q000382:microbiology; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001618:beta-Lactamases; C034413:beta-lactamase TEM-1