Comprehensive strategies to study neuronal function in transgenic animal models.
In the last 20 years of transgenic research, transgenic technology has been developed and refined to provide a seemingly comprehensive range of experimental approaches to questions of neuronal gene function; however, parallel developments in other technologies, such as whole genome sequencing and microarray analysis of gene expression, has meant that the questions have gotten harder. The complexity of neuronal systems and the difficulty of modeling neurologic diseases in rodents also present considerable challenges to molecular neuroscientists. Future functional genomic studies of brain and behavior will involve the full range of available transgenic methods and the incorporation of new technologies, including RNA interference.
Carter
David A
DA
School of Biosciences, Cardiff University, PO Box 911, Museum Avenue, Cardiff CF10 3US, Wales, UK.
D000818:Animals; D030801:Animals, Genetically Modified; D005818:Genetic Engineering; D051379:Mice; D023421:Models, Animal; D009474:Neurons / Q000502:physiology*; D010597:Pharmacogenetics; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D051381:Rats
D034741:RNA, Small Interfering