Thermodynamic characterization of the interaction of mutant UvrB protein with damaged DNA.
During the DNA damage recognition of nucleotide excision repair in Escherichia coli the interaction of UvrB protein with damaged DNA ensures the recognition of differences in the intrinsic chemical structures of a variety of adduct molecules in DNA double helix. Our earlier study indicated that a single tyrosine-to-tryptophan mutation at residue 95 converted the UvrB to a protein [UvrB(Y95W)] that is able to bind to a structure-specific bubble DNA substrate, even in the absence of UvrA. Fluorescence spectroscopy therefore was adopted to investigate the biochemical properties and thermodynamics of DNA damage recognition by the mutant protein. We examined the binding of the UvrB(Y95W) mutant protein to a structure-specific 30 bp DNA substrate containing a single fluorescein which serves as both an adduct and a fluorophore. Binding of the protein to the substrate results in a significant reduction in fluorescence. By monitoring the fluorescence changes, binding isotherms were generated from a series of titration experiments at various physiological temperatures, and dissociation constants were determined. Analysis of our data indicate that interaction of UvrB(Y95W) protein with the adduct incurred a large negative change in heat capacity DeltaC(p)(o)(obs) (-1.1 kcal mol(-1) K(-1)), while the DeltaG(o)(obs) was relatively unchanged with temperature. Further study of the binding at various concentrations of KCl showed that on average only about 1.5 ion pairs were involved in formation of the UvrB-DNA complex. Together, these results suggested that hydrophobic interactions are the main driving forces for the recognition of DNA damage by UvrB protein.
Ma
Huaxian
H
Department of Biochemistry and Molecular Biology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee 37614, USA.
Zou
Yue
Y
D020106:Acrylamide / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D018736:DNA Adducts / Q000737:chemistry; D004249:DNA Damage; D004265:DNA Helicases / Q000737:chemistry* / Q000235:genetics*; D004260:DNA Repair; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics*; D008956:Models, Chemical; D016297:Mutagenesis, Site-Directed; D009994:Osmolar Concentration; D011485:Protein Binding / Q000235:genetics; D013050:Spectrometry, Fluorescence; D013696:Temperature; D013816:Thermodynamics
D018736:DNA Adducts; D029968:Escherichia coli Proteins; C058093:UvrB protein, E coli; D020106:Acrylamide; D004247:DNA; D004265:DNA Helicases