title: Genomically intact endogenous feline leukemia viruses of recent origin.
abstract: We isolated and sequenced two complete endogenous feline leukemia viruses (enFeLVs), designated enFeLV-AGTT and enFeLV-GGAG. In enFeLV-AGTT, the open reading frames are reminiscent of a functioning FeLV genome, and the 5' and 3' long terminal repeat sequences are identical. Neither endogenous provirus is genetically fixed in cats but polymorphic, with 8.9 and 15.2% prevalence for enFeLV-AGTT and enFeLV-GGAG, respectively, among a survey of domestic cats. Neither provirus was found in the genomes of related species of the Felis genus, previously shown to harbor enFeLVs. The absence of mutational divergence, polymorphic incidence in cats, and absence in related species suggest that these enFeLVs may have entered the germ line more recently than previously believed, perhaps coincident with domestication, and reopens the question of whether some enFeLVs might be replication competent.
authors.affiliation: Laboratory of Genomic Diversity, Basic Research Program, SAIC-Frederick, Maryland 21702, USA.
authors.forename: Alfred L
authors.initials: AL
authors.lastname: Roca
authors.forename: Jill
authors.initials: J
authors.lastname: Pecon-Slattery
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: O'Brien
mesh_terms: D000818:Animals; D001483:Base Sequence; D002415:Cats / Q000235:genetics* / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D020077:Endogenous Retroviruses / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D019143:Evolution, Molecular; D016679:Genome, Viral; D005256:Leukemia Virus, Feline / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D011110:Polymorphism, Genetic; D020079:Terminal Repeat Sequences; D014779:Virus Replication / Q000235:genetics
chemical_list: D004279:DNA, Viral