Thermal preconditioning protected cerebellar granule neurons of rats by modulating HSP70 expression.
OBJECTIVE
To explore the possibility that expressions of different heat shock proteins (HSPs) are specifically involved in the protection of thermal preconditioning against apoptosis of cerebellar granule neurons (CGNs) induced by repolarization.


METHODS
Western blotting was used to detect expressions of HSP27, HSP70, and HSP90 induced by thermal preconditioning (TP) in CGNs; reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression level of HSP70 mRNA; apoptosis of CGNs was induced by switching culture medium containing KCl 25 mmol/L to one containing KCl 5 mmol/L.


RESULTS
No expression of HSP27 in cerebellar granule neurons was observed with TP at 44 degree. Expression of HSP90 was obvious in CGNs both without and with TP at 44 degree for different periods. Expression of HSP70 protein in CGNs was lower with TP at 44 degree for 5 min, but it increased gradually after the period was prolonged to 30, 60, or 90 min. HSP70 mRNA was detected after TP 44 degree for 30, 60, and 90 min and increased gradually with time. HSP70 antisense oligodeoxynucleotides 10 micromol/L for 72 h inhibited the protective effects of TP at 44 degree on apoptosis of CGNs induced by repolarization.


CONCLUSIONS
HSP70 is involved in protective effects of thermal preconditioning on apoptosis in cerebellar granule neurons induced by repolarization.
Chen
Li-jun
LJ
Department of Pharmacology, Zhongshan Medical College, Sun Yat-Sen University, Guangzhou 510089, China.
Su
Xing-wen
XW
Qiu
Peng-xin
PX
Huang
Yi-jun
YJ
Yan
Guang-mei
GM
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism; D006358:Hot Temperature; D008297:Male; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D020319:Oligodeoxyribonucleotides, Antisense; D012333:RNA, Messenger