Distribution of HIV-1 in the genomes of AIDS patients.
The localization of HIV-1 proviruses in compositional DNA fractions from 27 AIDS patients during the chronic phase of the disease with depletion of CD4+ and different levels of viremia showed the following. (1) At low viremia, proviruses are predominantly localized in the GC-richest isochores, which are characterized by an open chromatin structure; this result mimics findings on HIV-1 integration in early infected cells in culture. (2) At higher viremia, an increased distribution of proviruses in GC-poor isochores (which match the GC poorness of HIV-1) was found; this suggests a selection of cells in which the 'isopycnic' localization leads to a higher expression of proviruses and, in turn, to higher viremia. (3) At the highest viremia, integrations in GC-rich isochores are often predominant again, but generally not at the same level as in (1); this may be the consequence of new integrations from the extremely abundant RNA copies.
Tsyba
L
L
Laboratoire de Génétique Moléculaire, Institut Jacques Monod, Paris, France.
Rynditch
A V
AV
Boeri
E
E
Jabbari
K
K
Bernardi
G
G
D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics*; D001482:Base Composition; D015704:CD4 Antigens; D002843:Chromatin / Q000235:genetics; D002877:Chromosomes, Human; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D020862:GC Rich Sequence; D015894:Genome, Human; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D032085:Isochores; D011533:Proviruses / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D014766:Viremia / Q000235:genetics*; D016662:Virus Integration
D015704:CD4 Antigens; D002843:Chromatin; D004279:DNA, Viral; D032085:Isochores; D012367:RNA, Viral