title: Delayed hemolytic transfusion reaction due to anti-S antibody in patient with anti-Jk(a) autoantibody and multiple alloantibodies.
abstract: We describe the case of a 60-year-old woman with a delayed hemolytic transfusion reaction (DHTR). She had a history of an ulcerative colitis, blood transfusion because of rectal bleeding, and surgical removal of descendent and sigmoid colon. At admission, laboratory data showed Hb 6.3 g/dL, reticulocytes 120 x 10(9)/L, serum total bilirubin 1.2 mg/dL (direct bilirubin: 0.2 mg/dL). Pretransfusion antibody screening procedures were positive. A monospecific autoanti-Jk(a) and three alloantibodies (anti-c, -E, -K) were identified by immunohematologic studies. The patient received two units of crossmatch compatible concentrated red blood cells. Six days later biochemical serum values showed Hb 6.2 g/dL, LDH 975 I.U./L and total bilirubin 2.95 mg/dL (direct 0.35 mg/dL). Crossmatches with red cell suspension of transfused blood units and a post-transfusion serum were repeatedly positive. Laboratory tests showed the presence of anti-S alloantobody in the serum and eluate. Moreover, pre-transfusion serum of the patient was retrospectively retested: anti-S was not detected. These data suggested a DHTR. The present case is unusual and interesting because of the association of a rare autoanti-Jk(a), non responsible for anemia, and four alloantibodies of which anti-S involved in a DHTR.
authors.affiliation: Department of Hematology, Transfusion Medicine and Transplant Immunology, Second University of Naples, Naples, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Guastafierro
authors.forename: F
authors.initials: F
authors.lastname: Sessa
authors.forename: C
authors.initials: C
authors.lastname: Cuomo
authors.forename: A
authors.initials: A
authors.lastname: Tirelli
mesh_terms: D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D005260:Female; D006461:Hemolysis / Q000276:immunology*; D006801:Humans; D007518:Isoantibodies / Q000097:blood / Q000276:immunology*; D007667:Kidd Blood-Group System / Q000276:immunology*; D008951:MNSs Blood-Group System / Q000276:immunology*; D008875:Middle Aged; D013997:Time Factors; D065227:Transfusion Reaction
chemical_list: D001323:Autoantibodies; D007518:Isoantibodies; D007667:Kidd Blood-Group System; D008951:MNSs Blood-Group System