On the use of post-transcriptional processing elements in transgenes.
RNA processing events modulate final productivity of a given transgene. We have evaluated a series of RNA elements for their ability to enhance alpha1-antitrypsin production in mammary cells. Our results indicate the need for a case-by-case assessment of each construct design and the occurrence of gene silencing events in vivo.
Whitelaw
C B A
CB
Department of Gene Expression and Development, Roslin Institute (Edinburgh), Roslin, Midlothian, EH25 9PS, UK. bruce.whitelaw@bbsrc.ac.uk
Hiripi
L
L
Farini
E
E
Opsahl
M L
ML
Bosze
Z
Z
D000818:Animals; D020868:Gene Silencing; D018014:Gene Transfer Techniques; D051379:Mice; D008822:Mice, Transgenic; D012313:RNA / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D014162:Transfection; D019076:Transgenes
D012313:RNA