title: Lyme disease spirochete, Borrelia burgdorferi, endemic in epicenter at Turkey Point, Ontario.
abstract: The Lyme disease spirochete, Borrelia burgdorferi Johnson, Schmidt, Hyde, Steigerwalt, and Brenner, was discovered in blacklegged ticks, Ixodes scapularis Say at Turkey Point, Ontario, Canada. We report the first isolation of B. burgdorferi from a vertebrate animal collected on mainland Ontario. During this 2-yr study, spirochetes were isolated from the white-footed mouse, Peromyscus leucopus Rafinesque, and attached I. scapularis larvae. Similarly, isolates of B. burgdorferi were cultured from blacklegged tick adults, and confirmed positive with polymerase chain reaction by targeting OspA and rrf (5S) -rrl (23S) genes. Moreover, all isolates of B. burgdorferi from this area had complementary genetic structure, and the second primer set amplicons confirmed the first primer set amplification products. These findings show an epicenter endemic for B. burgdorferi within an established population of I. scapularis at Turkey Point.
authors.affiliation: Lyme Disease Association of Ontario, 365 St. David Street S., Fergus, Ontario, Canada N1M 2L7.
authors.forename: John D
authors.initials: JD
authors.lastname: Scott
authors.forename: Keerthi
authors.initials: K
authors.lastname: Fernando
authors.forename: Lance A
authors.initials: LA
authors.lastname: Durden
authors.forename: Muhammad G
authors.initials: MG
authors.lastname: Morshed
mesh_terms: D000818:Animals; D001483:Base Sequence; D025065:Borrelia burgdorferi / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers; D006801:Humans; D007814:Larva; D008193:Lyme Disease / Q000453:epidemiology*; D008322:Mammals / Q000382:microbiology; D009758:Nymph; D009864:Ontario / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods; D012621:Seasons; D013987:Ticks / Q000254:growth & development / Q000382:microbiology*
chemical_list: D017931:DNA Primers