The leader protein of Theiler's virus interferes with nucleocytoplasmic trafficking of cellular proteins.
The leader protein of Theiler's virus was previously shown to block the production of alpha/beta interferon by infected cells. Here, we observed that expression of the leader protein in infected cells triggered subcellular redistribution of a nucleus-target green fluorescent protein. It enhanced redistribution of the nuclear polypyrimidine tract-binding protein but had no influence on the localization of the nuclear splicing factor SC-35. The leader protein also interfered with trafficking of the cytoplasmic interferon regulatory factor 3, a factor critical for transcriptional activation of alpha/beta interferon genes.
Delhaye
Sophie
S
Christian de Duve Institute of Cellular Pathology, University of Louvain, MIPA-VIRO 74-49, B-1200 Brussels, Belgium.
van Pesch
Vincent
V
Michiels
Thomas
T
D021581:Active Transport, Cell Nucleus; D000818:Animals; D041702:BALB 3T3 Cells; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism / Q000821:virology; D004268:DNA-Binding Proteins / Q000378:metabolism; D049452:Green Fluorescent Proteins; D050838:Interferon Regulatory Factor-3; D008164:Luminescent Proteins / Q000378:metabolism; D051379:Mice; D009154:Mutation; D019913:Nuclear Localization Signals / Q000235:genetics / Q000502:physiology; D009687:Nuclear Proteins / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017945:Theilovirus / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism; D014764:Viral Proteins / Q000502:physiology*
D004268:DNA-Binding Proteins; D050838:Interferon Regulatory Factor-3; C494233:Irf3 protein, mouse; D008164:Luminescent Proteins; D019913:Nuclear Localization Signals; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D014764:Viral Proteins; D049452:Green Fluorescent Proteins