Influence of resin monomers on growth of oral streptococci.
Ethyleneglycol dimethacrylate monomers have been previously reported to stimulate the growth of certain caries-associated bacteria on the basis of turbidity measurements. To elucidate the detail of this effect, we examined the influence of resin monomers on the growth of Streptococcus sobrinus and Streptococcus sanguis by determination of bacterial numbers (colony-forming units), morphological observation, and chemical analysis. Although the absorbance values in the stationary phase of bacterial suspension were increased in the presence of ethyleneglycol monomers, no significant differences were observed for bacterial numbers throughout the incubation period. Scanning electron microscopy observation revealed the formation of sparse vesicular material surrounding bacterial cells when incubated with ethyleneglycol monomers, and these products were proved to be resin polymers. The results demonstrate that the apparent biomass increase during incubation with ethyleneglycol monomers is due not to promotion of bacterial multiplication, but to the polymerization of resin monomers to form vesicular structures attached to cells.
Takahashi
Y
Y
Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan. takahasi@dent.osaka-u.ac.jp
Imazato
S
S
Russell
R R B
RR
Noiri
Y
Y
Ebisu
S
S
D000179:Acrylates / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D017438:Bisphenol A-Glycidyl Methacrylate / Q000494:pharmacology; D015169:Colony Count, Microbial; D003188:Composite Resins / Q000494:pharmacology*; D005026:Ethylene Glycols / Q000494:pharmacology; D008689:Methacrylates / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D011109:Polymethacrylic Acids / Q000494:pharmacology; D011140:Polyurethanes / Q000494:pharmacology; D019279:Resin Cements / Q000494:pharmacology*; D013298:Streptococcus sanguis; D016986:Streptococcus sobrinus
D000179:Acrylates; D003188:Composite Resins; D005026:Ethylene Glycols; D008689:Methacrylates; D011109:Polymethacrylic Acids; D011140:Polyurethanes; D019279:Resin Cements; C029824:urethane dimethacrylate luting resin; C020946:triethylene glycol dimethacrylate; C047521:diethylene glycol dimethacrylate monomer; D011092:Polyethylene Glycols; D017438:Bisphenol A-Glycidyl Methacrylate