Facile synthesis of metal-chelating peptides on chip for protein array.
A unique peptide sequence of HGGHHG screening from a combinatorial synthetic peptide library showed a good chelating ability to bind a transition metal on a chip better than hexa-His peptide. It was directly conjugated with a His-Tagged proteins onto a chip in a mild aqueous solution and can be used this chip as a high throughput technique for protein array in order to detect and purify the His-Tagged proteins.
Cheng
Ching-Wen
CW
ALPS Biotech Co. Ltd, Taipei, Taiwan.
Lin
Kuo-Chin
KC
Pan
Fu-Ming
FM
Sinchaikul
Supachok
S
Wong
Chi-Huey
CH
Su
Wei-Chih
WC
Hsu
Ching-Hsiang
CH
Chen
Shui-Tein
ST
D002614:Chelating Agents / Q000138:chemical synthesis* / Q000378:metabolism; D002846:Chromatography, Affinity / Q000379:methods; D003300:Copper / Q000737:chemistry; D006639:Histidine / Q000737:chemistry; D008670:Metals / Q000737:chemistry*; D019151:Peptide Library; D010455:Peptides / Q000138:chemical synthesis* / Q000378:metabolism; D040081:Protein Array Analysis / Q000379:methods*; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D015032:Zinc / Q000737:chemistry
D002614:Chelating Agents; D008670:Metals; D019151:Peptide Library; D010455:Peptides; D011994:Recombinant Proteins; D006639:Histidine; D003300:Copper; D015032:Zinc