Determination of ginkgolide A, B, and bilobalide in biloba L. extracts by microdialysis-HPLC.
A method has been developed for measuring ginkgolide A, B, and bilobalide in ginkgo biloba L. extracts by using a self-assembled microdialysis device coupled on-line to high-performance liquid chromatography. The optimal conditions for dialysis efficiency and chromatographic analysis were investigated. The results show that the dialysis efficiencies for ginkgolide A, B, and bilobalide are 97.8-100.4%. For the spike concentration of 45 mg L(-1), the recoveries of ginkgolide A, B, and bilobalide in ginkgo biloba L. extracts were at 97-100.7% with 3.1-4.7% RSD. The proposed method has therefore been shown to be applicable to the analysis of ginkgolide A, B, and bilobalide in ginkgo biloba L. extracts.
Chiu
Houn-Lin
HL
Department of Chemistry, National Kaohsiung Normal University, 116 Ho-Ping 1st Rd., Kaohsiung, Taiwan.
Lin
Hui-Ya
HY
Yang
Thomas Ching-Cherng
TC
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003517:Cyclopentanes / Q000302:isolation & purification*; D004224:Diterpenes / Q000302:isolation & purification*; D005663:Furans / Q000302:isolation & purification*; D020441:Ginkgo biloba / Q000737:chemistry*; D046934:Ginkgolides; D007783:Lactones / Q000302:isolation & purification*; D017551:Microdialysis; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry
D003517:Cyclopentanes; D004224:Diterpenes; D005663:Furans; D046934:Ginkgolides; D007783:Lactones; D010936:Plant Extracts; C045856:ginkgolide B; C073710:bilobalide; C477042:ginkgolide A