title: Use of 2,4-dinitrophenylhydrazone of glyoxylic acid for the determination of glyoxylic acid by the chromatographic-spectrophotometric method and by differential pulse polarography.
abstract: Two different methods (one based on chromatography and spectrophotometry and the other on polarography) have been developed for the determination of glyoxylic acid in the form of a derivative with 2,4-dinitrophenylhydrazine (DNPH. GA). The TLC method allows the separation of two DNPH. GA isomers ("trans" and "cis"). Spectrophotometric measurements of the eluents of the separated compounds (lambda=360 nm) allow the determination of GA within the range from 4 to 30 microg. Using differential pulse polarography, the conditions of DNPH. GA formation were examined. The reduction peak of this derivative (E(P)=-0.430 V), observed by dpp, was used to develop a polarographic determination of GA within the concentration range from 1.10(-4) to 7.10(-4) mol/l.
authors.affiliation: Institute of General Food Chemistry, Technical University of Łódź, 4/10 B. Stefanowskiego St., PL-90924, Łódź, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Masłowska
authors.forename: J
authors.initials: J
authors.lastname: Duda
authors.forename: A
authors.initials: A
authors.lastname: Witusik