[Amplification of Lactobacillus bulgaricus beta-galactosidase gene and optimization of the reaction condition].
OBJECTIVE
To obtain the gene which encode high activity beta-galactosidase from Lactobacillus bulgaricus and study the influencing factors of amplification.


METHODS
Lysozyme and freeze-thaw cycles were applied to obtain Lactobacillus bulgaricus DNA; different template concentration and annealing temperature were selected for the amplification.


RESULTS
60 ng/microliter template concentration and 66 degrees C annealing temperature were the best reaction conditions of amplifying the gene.


CONCLUSIONS
This study optimized the template concentration and anneal temperature conditions of amplifying Lactobacillus bulgaricus beta-galactosidase gene, thus providing a foundation for further studies.
Wang
Chuan
C
Department of Medical Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Zhang
Chao-wu
CW
Pei
Xiao-fang
XF
Yu
Qian
Q
Lü
Xiao-ying
XY
D004269:DNA, Bacterial / Q000235:genetics; D005784:Gene Amplification; D007778:Lactobacillus / Q000201:enzymology*; D009113:Muramidase / Q000235:genetics; D016133:Polymerase Chain Reaction; D013698:Templates, Genetic; D001616:beta-Galactosidase / Q000235:genetics*
D004269:DNA, Bacterial; D009113:Muramidase; D001616:beta-Galactosidase