title: Disruption of the gene (spo0A) encoding sporulation transcription factor blocks endospore formation and enterotoxin production in enterotoxigenic Clostridium perfringens type A.
abstract: This study identified a functional spo0A ORF in enterotoxigenic Clostridium perfringens type A. To evaluate the function of spo0A, an isogenic spo0A knock-out mutant was constructed. The spo0A mutant was unable to form endospores and produce enterotoxin, however, these defects could be restored by complementing the mutant with a recombinant plasmid carrying the wild-type spo0A gene. These results provide evidence that spo0A expression is essential for sporulation and enterotoxin production in C. perfringens.
authors.affiliation: Department of Microbiology, Oregon State University, 220 Nash Hall, Corvallis, OR 97331, USA.
authors.forename: I-Hsiu
authors.initials: IH
authors.lastname: Huang
authors.forename: Michael
authors.initials: M
authors.lastname: Waters
authors.forename: Roberto R
authors.initials: RR
authors.lastname: Grau
authors.forename: Mahfuzur R
authors.initials: MR
authors.lastname: Sarker
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003016:Clostridium perfringens / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D004768:Enterotoxins / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010957:Plasmids; D013171:Spores, Bacterial / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004768:Enterotoxins; C087737:Spo0H protein, Bacillus subtilis; D014157:Transcription Factors; C035598:enterotoxin, Clostridium