Yeast differentiation using histone promoter sequences.
OBJECTIVE
To evaluate a new platform for yeast differentiation based on histone promoter regions.


RESULTS
The histone gene amino acid sequences of a wide phylogenetic range of organisms were aligned, and primers designed that were capable of amplifying the divergent promoters of the H3-H4 and H2a-H2b loci from yeast. Analysis indicated that the promoter regions were variable in length between species and represented rapidly changing sequences flanked by highly conserved sequences.


CONCLUSIONS
The histone promoter regions in yeast provide an excellent locus for the rapid and accurate identification of yeast species.


CONCLUSIONS
This study describes an alternative platform to the ribosomal internal transcribed spacer (ITS) sequences for the identification of yeast species.
Bell
P J L
PJ
Microbiogen Pty Ltd, Macquarie University Campus, Sydney, NSW, Australia. pbell@rna.bio.mq.edu.au
D001483:Base Sequence; D004271:DNA, Fungal / Q000032:analysis; D006657:Histones / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D004718:Saccharomycetales / Q000145:classification* / Q000235:genetics*
D004271:DNA, Fungal; D006657:Histones