title: Evidence for prostate cancer-associated diagnostic marker-1: immunohistochemistry and in situ hybridization studies.
abstract: OBJECTIVE
  The purpose of this study was to characterize a novel gene/protein associated with prostate cancer, termed prostate cancer-associated diagnostic marker-1 [PCADM-1 (Hu Y, Wang M, Garcia FU, Aoyaki K, Stearns ME. Identification of PCADM-1 as a novel diagnostic marker for prostate cancer, submitted for publication)].
  
  
  RESULTS
  Immunological studies revealed that rabbit polyclonal antibodies generated against recombinant PCADM-1 specifically recognize the protein in crude protein extracts from a variety of prostate cancer cell lines (i.e., PC-3 ML, LNCaP, DU145, and CPTX-1532) and prostate cancer tissue. Combined immunolabeling and in situ hybridization studies demonstrated that PCADM-1 mRNA was expressed by the luminal epithelial cells of prostate cancer glands and was not expressed by high-grade prostatic intraepithelial neoplasia or HPV-MLC7 cells. Immunolabeling studies of tissue arrays from biopsies of archival material (n = 200 samples) confirmed that PCADM-1 was expressed by the luminal epithelial cells of prostate cancer.
  
  
  CONCLUSIONS
  Taken together, the data suggest that PCADM-1 is a specific marker for human prostate cancer.
authors.affiliation: Drexel University College of Medicine, Department of Pathology and Laboratory Medicine, Philadelphia, Pennsylvania 19102, USA.
authors.forename: Akira
authors.initials: A
authors.lastname: Ohkia
authors.forename: Youji
authors.initials: Y
authors.lastname: Hu
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Fernando U
authors.initials: FU
authors.lastname: Garcia
authors.forename: Mark E
authors.initials: ME
authors.lastname: Stearns
mesh_terms: D000951:Antigens, Neoplasm / Q000096:biosynthesis* / Q000235:genetics*; D014408:Biomarkers, Tumor; D015153:Blotting, Western; D002460:Cell Line; D045744:Cell Line, Tumor; D006801:Humans; D006965:Hyperplasia; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D011467:Prostate / Q000378:metabolism; D019048:Prostatic Intraepithelial Neoplasia / Q000473:pathology; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D012984:Software
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D011994:Recombinant Proteins