title: Cytometric bead array: a multiplexed assay platform with applications in various areas of biology.
abstract: The introduction of flow cytometric bead-based technology has added a new approach for investigators to simultaneously measure multiple analytes in biological and environmental samples. This new technology allows for (1) evaluation of multiple analytes in a single sample; (2) utilization of minimal sample volumes to glean data; (3) reproducibility and results comparative with previous experiments; (4) direct comparison with existing assays; and (5) a more rapid evaluation of multiple samples in a single platform. The cytometric bead array (CBA) system enables simultaneous measurement of multiple analytes in sample volumes too small for traditional immunoassays. Results have been presented for the analysis of a variety of human cytokines. In addition, the technology allows for the design and creation of assays to measure a variety of analytes including inflammatory mediators, chemokines, immunoglobulin isotypes, intracellular signaling molecules, apoptotic mediators, adhesion molecules, and antibodies. New initiatives put forward by the Human Genome Project and the FDA require the development and use of assays for the rapid simultaneous quantitation of multiple analytes. The CBA technology provides the ability to quantify multiple proteins within a given sample, with precision and consistency.
authors.affiliation: BD Biosciences, San Diego, CA 92121, USA.
authors.forename: Edward
authors.initials: E
authors.lastname: Morgan
authors.forename: Rudi
authors.initials: R
authors.lastname: Varro
authors.forename: Homero
authors.initials: H
authors.lastname: Sepulveda
authors.forename: Julia A
authors.initials: JA
authors.lastname: Ember
authors.forename: John
authors.initials: J
authors.lastname: Apgar
authors.forename: Jerry
authors.initials: J
authors.lastname: Wilson
authors.forename: Larry
authors.initials: L
authors.lastname: Lowe
authors.forename: Roy
authors.initials: R
authors.lastname: Chen
authors.forename: Lalita
authors.initials: L
authors.lastname: Shivraj
authors.forename: Anissa
authors.initials: A
authors.lastname: Agadir
authors.forename: Roberto
authors.initials: R
authors.lastname: Campos
authors.forename: David
authors.initials: D
authors.lastname: Ernst
authors.forename: Amitabh
authors.initials: A
authors.lastname: Gaur
mesh_terms: D000706:Anaphylatoxins / Q000032:analysis / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology; D017209:Apoptosis / Q000276:immunology; D020169:Caspases / Q000032:analysis / Q000276:immunology; D016207:Cytokines / Q000032:analysis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008863:Microspheres; D010770:Phosphotransferases / Q000032:analysis / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000706:Anaphylatoxins; D000906:Antibodies; D016207:Cytokines; D010770:Phosphotransferases; D020169:Caspases