title: Increased state 4 mitochondrial respiration and swelling in early post-ischemic reperfusion of rat heart.
abstract: Isolated rat hearts were exposed to 30 min ischemia or to 30 min ischemia followed by 2, 5 or 40 min reperfusion and mitochondria were isolated at these different time points. ADP-stimulated, succinate-dependent respiration rate (state 3) was not significantly changed at the different time points examined. In contrast, state 4 (non-ADP-stimulated) respiration rate was significantly increased after 30 min ischemia, and it increased further during the first post-ischemic reperfusion period. Mitochondrial swelling, as evaluated under conditions of the major controlled ion channels (i.e. permeability transition pore and ATP-dependent mitochondrial K(+) channel) closed, significantly increased in parallel. It is suggested that the inner mitochondrial membrane permeability is increased under exposure of the heart to ischemia and early reperfusion, and that the phenomenon is reversible upon subsequent long periods of reperfusion.
authors.affiliation: Scuola Superiore di Studi Universitari e di Perfezionamento S. Anna, Pisa, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Bosetti
authors.forename: A
authors.initials: A
authors.lastname: Baracca
authors.forename: G
authors.initials: G
authors.lastname: Lenaz
authors.forename: G
authors.initials: G
authors.lastname: Solaini
mesh_terms: D000251:Adenosine Triphosphatases / Q000032:analysis; D000818:Animals; D019069:Cell Respiration; D004487:Edema / Q000503:physiopathology*; D003576:Electron Transport Complex IV / Q000032:analysis; D008297:Male; D008929:Mitochondria, Heart / Q000378:metabolism*; D017202:Myocardial Ischemia / Q000473:pathology / Q000503:physiopathology*; D015425:Myocardial Reperfusion; D010101:Oxygen Consumption; D015221:Potassium Channels / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D015221:Potassium Channels; D003576:Electron Transport Complex IV; D000251:Adenosine Triphosphatases