Capillary electrophoresis and capillary flow injection analysis with electrochemical detection.
Measurements by capillary flow injection analysis (CFIA) and capillary electrophoresis (CE) in conjunction with electrochemical detection are described. The detection is based on an end-column electrode arrangement. Several novel electrodes, such as a spherical gold electrode and a dual-microdisk electrode, are presented and characterized regarding their analytical utility. In order to improve the selectivity of CFIA, dual-electrode and multiple-pulse detection are studied using couples of cyanometallates or metallocenes. Capillary electrophoretic experiments with amperometric detection are performed using 50 microm i.d. capillaries without any electrical-field decoupler. The practicality and analytical characteristics of this detection strategy are illustrated for the separation of serotonin and some biological precursors and metabolites of neurotransmitter substances.
Matysik
F M
FM
Departamento de Química, Universidade de Coimbra, P-3049, Coimbra, Portugal.
Backofen
U
U