title: [Amplification of Lactobacillus bulgaricus beta-galactosidase gene and optimization of the reaction condition].
abstract: OBJECTIVE
  To obtain the gene which encode high activity beta-galactosidase from Lactobacillus bulgaricus and study the influencing factors of amplification.
  
  
  METHODS
  Lysozyme and freeze-thaw cycles were applied to obtain Lactobacillus bulgaricus DNA; different template concentration and annealing temperature were selected for the amplification.
  
  
  RESULTS
  60 ng/microliter template concentration and 66 degrees C annealing temperature were the best reaction conditions of amplifying the gene.
  
  
  CONCLUSIONS
  This study optimized the template concentration and anneal temperature conditions of amplifying Lactobacillus bulgaricus beta-galactosidase gene, thus providing a foundation for further studies.
authors.affiliation: Department of Medical Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Wang
authors.forename: Chao-wu
authors.initials: CW
authors.lastname: Zhang
authors.forename: Xiao-fang
authors.initials: XF
authors.lastname: Pei
authors.forename: Qian
authors.initials: Q
authors.lastname: Yu
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Lü
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D005784:Gene Amplification; D007778:Lactobacillus / Q000201:enzymology*; D009113:Muramidase / Q000235:genetics; D016133:Polymerase Chain Reaction; D013698:Templates, Genetic; D001616:beta-Galactosidase / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D009113:Muramidase; D001616:beta-Galactosidase