Glucocorticoid-inducible gene expression vectors for use in Drosophila melanogaster Schneider 2 cells.
Inducible, vector-based, expression systems that allow fine control of transgene expression are gaining more and more use in fundamental research as well as in therapeutic applications. In an effort to develop a tightly regulated heterologous expression system for Drosophila Schneider 2 cells, three different inducible reporter constructs were compared. These comprised six copies of the glucocorticoid response element fused to one of three distinct types of Drosophila gene promoters: (1) a TATA-box containing, (2) a TATA-less and (3) a bidirectional core sequence. These were fused to a luciferase reporter gene. The promoter constructs displayed different basal as well as agonist-induced activities. The implications of the observations made are discussed in the context of promoter properties and of induction of genes that may be studied in Drosophila.
Poels
J
J
Laboratory for Developmental Physiology, Genomics and Proteomics, Leuven, Belgium.
Martinez
A
A
Suner
M-M
MM
De Loof
A
A
Dunbar
S J
SJ
Vanden Broeck
J
J
D000818:Animals; D002478:Cells, Cultured; D017931:DNA Primers; D004331:Drosophila melanogaster / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism; D005938:Glucocorticoids / Q000378:metabolism*; D008156:Luciferases / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D019076:Transgenes / Q000235:genetics
D017931:DNA Primers; D005938:Glucocorticoids; D008156:Luciferases