Production of L-phenylacetyl carbinol by immobilized cells of Saccharomyces cerevisiae.
Conversion of benzaldehyde to L-phenylacetyl carbinol (L-PAC) was achieved with immobilized, growing cells of Saccharomyces cerevisiae in different reactors. Product formation increased (31%) with the subsequent initial reuses of the entrapped cells. Biomass production and PAC formation depleted (40 and 57%, respectively) after 4-5 continuous growth and biotransformation cycles. With the regeneration of the biocatalysts, catalytic activity of the cells was resumed. The highest yields were in a stirred tank reactor (29 g PAC) from 77 g benzeldehyde with 14 repeated uses of entrapped cells.
Mandwal
A K
AK
Division of Fermentation Technology, Central Drug Research Institute, Lucknow 226 001, India.
Tripathi
C K M
CK
Trivedi
P D
PD
Joshi
A K
AK
Agarwal
S C
SC
Bihari
Vinod
V
D000096:Acetone / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism*; D001547:Benzaldehydes / Q000378:metabolism*; D019149:Bioreactors / Q000145:classification / Q000382:microbiology*; D001711:Biotransformation; D002384:Catalysis; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D018914:Cells, Immobilized / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*
D001547:Benzaldehydes; D000096:Acetone; C035293:1-hydroxy-1-phenyl-2-propanone; C032175:benzaldehyde