title: Rapid time-resolved fluoroimmunoassay for the screening of narasin and salinomycin residues in poultry and eggs.
abstract: Anticoccidial drugs are extensively used in the poultry industry to control the infection of the single-cell protozoa of the genus Eimeria. The most commonly used coccidiostats in poultry are the polyether ionophores such as narasin and salinomycin. This paper presents a rapid and simple method for the screening of residues of these two coccidiostatic compounds in poultry and eggs. The method is based on time-resolved fluoroimmunoassay. Sample preparation of eggs consists only of one extraction and evaporation step, and a solid phase extraction step is needed only for the muscle sample preparation. Mean recoveries were 91.0% from muscle tissue and 81.1% from eggs for both narasin and salinomycin. The performance of the assay was evaluated only for narasin because salinomycin had a cross-reactivity of 100% in the assay, and the recoveries of the compounds were not significantly different (P >0.05). The limits of detection [mean + 3 x standard deviation (SD)] of narasin were 0.56 and 0.28 microg/kg, and the limits of quantification (mean + 9 x SD) were 1.80 and 0.57 microg/kg for muscle and eggs, respectively. The coefficients of variation (CV) of the interassay precision of the method, evaluated by five replicate analyses of muscle samples spiked with 2 microg/kg of narasin and egg samples spiked with 1 microg/kg of narasin, were 4.1 and 6.4%, respectively. The CVs of intra-assay precision tests, determined by 10 replicate analyses at the above-mentioned concentration levels, were 3.8 and 4.5%, respectively.
authors.affiliation: Department of Biotechnology, University of Turku, Biocity 6a, FIN-20520 Turku, Finland. pekka.peippo@utu.fi
authors.forename: Pekka
authors.initials: P
authors.lastname: Peippo
authors.forename: Virve
authors.initials: V
authors.lastname: Hagren
authors.forename: Timo
authors.initials: T
authors.lastname: Lövgren
authors.forename: Mika
authors.initials: M
authors.lastname: Tuomola
mesh_terms: D000818:Animals; D002645:Chickens; D003049:Coccidiostats / Q000032:analysis*; D004531:Eggs / Q000032:analysis*; D015200:Fluoroimmunoassay / Q000379:methods*; D008460:Meat / Q000032:analysis; D009132:Muscles / Q000737:chemistry; D011714:Pyrans / Q000032:analysis*; D012680:Sensitivity and Specificity; D000095662:Polyether Polyketides
chemical_list: D003049:Coccidiostats; D011714:Pyrans; C010327:salinomycin; D000095662:Polyether Polyketides; C013612:narasin