title: Nuclear and chloroplast DNA differentiation in Andean potatoes.
abstract: Over 3500 accessions of Andean landraces have been known in potato, classified into 7 cultivated species ranging from 2x to 5x (Hawkes 1990). Chloroplast DNA (ctDNA), distinguished into T, W, C, S, and A types, showed extensive overlaps in their frequencies among cultivated species and between cultivated and putative ancestral wild species. In this study, 76 accessions of cultivated and 19 accessions of wild species were evaluated for ctDNA types and examined by ctDNA high-resolution markers (ctDNA microsatellites and H3 marker) and nuclear DNA restriction fragment length polymorphisms (RFLPs). ctDNA high-resolution markers identified 25 different ctDNA haplotypes. The S- and A-type ctDNAs were discriminated as unique haplotypes from 12 haplotypes having C-type ctDNA and T-type ctDNA from 10 haplotypes having W-type ctDNA. Differences among ctDNA types were strongly correlated with those of ctDNA high-resolution markers (r = 0.822). Differentiation between W-type ctDNA and C-, S-, and A-type ctDNAs was supported by nDNA RFLPs in most species except for those of recent or immediate hybrid origin. However, differentiation among C-, S-, and A-type ctDNAs was not clearly supported by nDNA RFLPs, suggesting that frequent genetic exchange occurred among them and (or) they shared the same gene pool owing to common ancestry.
authors.affiliation: Graduate School of Science and Technology, Kobe University, Kobe, Japan.
authors.forename: Thitaporn
authors.initials: T
authors.lastname: Sukhotu
authors.forename: Osamu
authors.initials: O
authors.lastname: Kamijima
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Hosaka
mesh_terms: D017931:DNA Primers; D018742:DNA, Chloroplast / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D006239:Haplotypes / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics; D010568:Peru; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D011198:Solanum tuberosum / Q000235:genetics*; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018742:DNA, Chloroplast; D005819:Genetic Markers