Effects of low dose aspirin (81 mg) on proliferating cell nuclear antigen and Amaranthus caudatus labeling in normal-risk and high-risk human subjects for colorectal cancer.
Epidemiological, experimental, and clinical observations provide support for a colorectal cancer chemopreventive role for aspirin. We have evaluated the effects of aspirin on proliferation biomarkers in normal-risk and high-risk human subjects for colorectal cancer. Colorectal biopsies were obtained at baseline and at 24h after 28 daily doses of 81 mg of aspirin from 13 high-risk and 15 normal-risk subjects for colorectal cancer. We evaluated aspirin's effects on proliferating cell nuclear antigen (PCNA) immunohistochemistry and epithelial mucin histochemistry using the lectin, Amaranthus caudatus agglutinin (ACA) in crypt sections from rectal biopsies. The baseline whole crypt PCNA LIs differed significantly between normal-risk and high-risk subjects. PCNA LIs are not affected by 28 days of aspirin at 81 mg daily. ACA LIs are decreased by 28 days of aspirin at 81 mg daily in both normal-risk and high-risk subjects. Aspirin's effects on ACA LIs may have mechanistic and biological implications that deserve further attention. PCNA and ACA LIs are not useful as proliferation biomarkers for aspirin's chemopreventive activity in morphologically normal human colorectal mucosa.
Krishnan
Koyamangalath
K
Medical Service, James H. Quillen Veterans Administration Medical Center and Division of Hematology-Oncology, Department of Internal Medicine, East Tennessee State University, Johnson City, TN 37614, USA.
Aoki
Toshihiro
T
Ruffin
Mack T
MT
Normolle
Daniel P
DP
Boland
C Richard
CR
Brenner
Dean E
DE
D000328:Adult; D000368:Aged; D016588:Anticarcinogenic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001241:Aspirin / Q000008:administration & dosage / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D003106:Colon / Q000166:cytology / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000517:prevention & control; D004847:Epithelial Cells / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006801:Humans; D037102:Lectins; D008297:Male; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen / Q000187:drug effects* / Q000378:metabolism; D013194:Staining and Labeling
D016588:Anticarcinogenic Agents; D037102:Lectins; D018809:Proliferating Cell Nuclear Antigen; C422071:lectin, Amaranthus caudatus; D001241:Aspirin