Changes of GABA(A)receptor activation kinetics in hippocampal neurons cultured for different periods of time.
Cell culture is a convenient model for pharmacokinetic studies, but during the culture period, GABA(A)receptors are likely to undergo different modulatory processes. In this study, the current responses to ultrafast GABA applications were recorded from patches excised from neurons cultured for either up to two days (short-term culture) or for more than two weeks (long-term culture). The dose-dependencies of the current rising phases revealed significant differences between the two groups. In the short-term cultures, the responses to both saturating and non-saturating GABA concentrations were slower than in the case of the long-term cultures. We conclude that the GABA(A)receptors in cultured neurons undergo profound kinetic changes involving the modulation of the binding reaction and transitions between bound states.
Mozrzymas
Jerzy W
JW
Department of Biophysics, Wrocław Medical University, Chałubińskiego 10, 50-368 Wrocław, Poland. mozrzy@biofiz.am.wroc.pl
Barberis
Andrea
A
D000818:Animals; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D006624:Hippocampus / Q000502:physiology*; D015640:Ion Channel Gating / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time / Q000502:physiology; D011963:Receptors, GABA-A / Q000502:physiology*
D011963:Receptors, GABA-A