[Changes of connexin 43 in rabbit with early myocardial ischemia].
OBJECTIVE
To reveal gap junction connexin 43 (Cx43) in rabbit with early myocardial ischemia.


METHODS
The left coronary arteries were ligated to create early myocardial ischemia. Cx43 was visualized by use of SP immunohistochemical method (IHC) and Colloidal gold of the electron microscopy, and a comparison was made between the experiment group and the control group. The semi-quantitation assessment was performed.


RESULTS
The positive areas of Cx43 gap junctions by IHC and Colloidal gold particles of the electron microscopy were found decreased by about 40% at 30 minutes of acute myocardial ischemia. The distributions were not obviously modified.


CONCLUSIONS
The decrease of Cx43 is characterized by the time during myocardial ischemia, and the early stage of acute myocardial ischemia in rat is of positive significance.
Chen
Yang
Y
Department of Forensic Pathology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Hu
Hua-zi
HZ
Wu
Jia-wen
JW
Peng
Qi-yi
QY
Yang
Guo
G
Liao
Zhi-gang
ZG
D000818:Animals; D018031:Connexin 43 / Q000378:metabolism*; D005260:Female; D017629:Gap Junctions / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure; D011817:Rabbits; D011897:Random Allocation; D013997:Time Factors
D018031:Connexin 43