Differentiation of classical and very virulent strains/isolates of Infectious bursal disease virus by reverse transcription-polymerase chain reaction.
Reverse transcription-polymerase chain reaction (RT-PCR) is a rapid method for identification and differentiation of viruses. It was used to differentiate very virulent from classical (field/vaccine) strains/isolates of Infectious bursal disease virus (IBDV). RT-PCR products of 552 bp were generated by amplification of variable region of VP2 gene in three field classical isolates, two vaccine strains and two very virulent isolates of IBDV. The PCR products were digested with SacI, HhaI, SspI and StuI. Digestion of the PCR products with SacI and HhaI revealed the presence of a single restriction site in all the field classical isolates and vaccine strains, but no such a restriction site in very virulent strains. On the other hand digestion of these products with SspI and StuI showed the presence of a single restriction site in very virulent strains but no such a restriction site in classical field isolates and vaccine strains. Although the restriction profiles of classical field Indian isolates and vaccine strains were identical, all of these enzymes could differentiate very virulent Indian strains from classical field isolates and vaccine strains.
Toroghi
R
R
Division of Avian Diseases, Indian Veterinary Research Institute, Izatnagar, UP 243122, India. rtoroghi@rvsri.com
Kataria
J M
JM
Balamurugan
V
V
D000818:Animals; D001483:Base Sequence; D018175:Birnaviridae Infections / Q000662:veterinary / Q000821:virology; D002645:Chickens; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D007194:India; D007243:Infectious bursal disease virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D011201:Poultry Diseases / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015678:Viral Structural Proteins / Q000235:genetics; D014765:Viral Vaccines / Q000235:genetics; D014774:Virulence / Q000235:genetics
D004279:DNA, Viral; C082513:VP2 protein, infectious bursal disease virus; D015678:Viral Structural Proteins; D014765:Viral Vaccines