title: The yeast production system in which Escherichia coli phytase is expressed may affect growth performance, bone ash, and nutrient use in broiler chicks.
abstract: The efficacy of three Escherichia coli-derived phytase preparations on the performance and nutrient utilization of broiler chicks was evaluated. Two hundred sixteen 7-d-old male broiler chicks were grouped by weight into 6 blocks of 6 cages with 6 birds per cage. Six corn-soybean meal-based diets were randomly assigned to cages within each block. The 6 diets were adequate P, very low P, and low P and contained (g of P/kg of diet) 7.7, 4.0, and 5.1, respectively; and low-P diet plus phytase preparation A, B, or C at 1,000 units/kg of feed. All 3 phytase preparations were produced in different yeast production systems with slightly different glycosylation patterns. Preparation A was produced in Pichia pastoris, B in Schizosaccharomyces pombe, and C in Saccharomyces cerevisiae. The chicks were fed the experimental diets from 8 to 22 d of age. Excreta samples were collected between 17 and 21 d of age. At the end of the study, blood was collected, chicks were killed, and tibiae were removed from 3 birds per cage. Weight gain, feed intake, and feed efficiency among the 3 phytase preparations did not differ, although only phytase A diet outperformed (P < 0.05) the low-P diet in terms of weight gain and feed efficiency. All 3 phytase diets outperformed (P < 0.05) the low-P diet in bone mineral content, density, strength, percentage ash, P retention, and serum P levels. Phytase B diet outperformed the adequate-P diet in bone strength. All 3 preparations increased (P < 0.05) Ca retention with phytase B or C showing a better retention of Ca than phytase A. All 3 phytase preparations showed similar P use as indicated by BW gain and tibia bone characteristics.
authors.affiliation: Department of Animal Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: E M
authors.initials: EM
authors.lastname: Onyango
authors.forename: M R
authors.initials: MR
authors.lastname: Bedford
authors.forename: O
authors.initials: O
authors.lastname: Adeola
mesh_terms: D010832:6-Phytase / Q000008:administration & dosage* / Q000235:genetics*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D015519:Bone Density; D001842:Bone and Bones / Q000737:chemistry / Q000502:physiology; D002645:Chickens / Q000254:growth & development / Q000502:physiology*; D004926:Escherichia coli / Q000201:enzymology; D015870:Gene Expression; D008297:Male; D010758:Phosphorus / Q000032:analysis / Q000097:blood; D016226:Phosphorus, Dietary / Q000008:administration & dosage / Q000378:metabolism; D010843:Pichia / Q000378:metabolism; D011994:Recombinant Proteins; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012568:Schizosaccharomyces / Q000378:metabolism; D013977:Tibia / Q000737:chemistry / Q000502:physiology; D015430:Weight Gain; D015003:Yeasts / Q000378:metabolism*
chemical_list: D016226:Phosphorus, Dietary; D011994:Recombinant Proteins; D010758:Phosphorus; D010832:6-Phytase