Survival advantage of cultured human vascular endothelial cells that lost chromosome 13.
We explored the nature of chromosome 13 loss in cultured human vascular endothelial cells (HUVECs). Chromosome 13 loss detected by metaphase and interphase analysis was noted in earlier passages of HUVEC strains with no relation to telomere length or replicative senescence. Ectopic expression of telomerase did not influence the loss of chromosome 13. HUVECs losing chromosome 13 demonstrated increased migratory potential and loss of heterozygosity. Collectively, these observations suggest that the loss of chromosome 13 gives cultured HUVECs a replicative advantage.
Kimura
Masayuki
M
Hypertension Research Center, Cardiovascular Research Institute, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, NJ 07103, USA.
Cao
Xiaojian
X
Patel
Smita
S
Aviv
Abraham
A
D002452:Cell Count; D002470:Cell Survival; D002478:Cells, Cultured; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics* / Q000378:metabolism; D004261:DNA Replication; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology; D006801:Humans; D019656:Loss of Heterozygosity; D012150:Polymorphism, Restriction Fragment Length; D019098:Telomerase / Q000378:metabolism
D019098:Telomerase