[The mRNA expression of platelet-derived endothelial cell growth factor of bladder transitional cell carcinoma and its relation to invasiveness].
OBJECTIVE
To investigate the relationship between mRNA expression of platelet-derived endothelial cell growth factor (PD-ECGF) and invasion of bladder transitional cell carcinoma (BTCC).


METHODS
The mRNA expression of PD-ECGF in BTCC was detected by RT-PCR. The target PCR bands were analyzed by NIH Image 1.62 software.


RESULTS
The mRNA level of PD-ECGF in BTCC was 3.86 times as high as that of normal bladder mucosa (t = 2.36, P < 0.05). The expression level of stage Ta, T1 and T2-4 tumor was 1.33, 4.02 and 7.59 times as high as that of normal bladder mucosa, respectively. That of Grade 3 tumor was 2.27 times as high as that of Grade 1 - 2 tumor (t = 3.52, P < 0.01).


CONCLUSIONS
The mRNA expression of PD-ECGF was positively correlated with the invasiveness and grade of BTCC. The results suggest that the mRNA level of PD-ECGF might be used as an indicator of tumor progression and a guide for clinical treatment of bladder transitional cell carcinoma.
Li
Gang
G
Department of Urology, First Affiliated Hospital, Soochow University, Suzhou 215006, China.
Zhang
Yuan-fang
YF
Ding
Qiang
Q
Wen
Duan-gai
DG
Yan
Chun-yin
CY
D000328:Adult; D000368:Aged; D002295:Carcinoma, Transitional Cell / Q000378:metabolism* / Q000473:pathology; D002454:Cell Differentiation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000032:analysis*; D013939:Thymidine Phosphorylase / Q000235:genetics*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology
D012333:RNA, Messenger; D013939:Thymidine Phosphorylase