Identification of the anginosus group within the genus Streptococcus using polymerase chain reaction.
The aim of this study was to establish an identification method for the anginosus group within the genus Streptococcus by polymerase chain reaction (PCR). Using a primer pair based on the group-specific sequences of penicillin-binding protein 2B (pbp2b) gene, a 275-bp fragment was amplified from each species in the group but no size-matched products were obtained in other streptococci. Further identification in the species or subspecies level was possible by a multiplex PCR with primers for the 16S ribosomal RNA gene of Streptococcus anginosus, the hyaluronate lyase genes both of Streptococcus intermedius and Streptococcus constellatus subsp. constellatus, and the intermedilysin (ily) gene of S. intermedius. In the case ofStreptococcus constellatus subsp. pharyngis, the amplified fragment from the S. intermedius-type hyaluronate lyase gene was obtained, while that from the ily gene was not. These results also indicate that two different hyaluronate lyase genes are distributed among the anginosus group.
Takao
Ayuko
A
Department of Oral Bacteriology, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan. takao-a@tsurumi-u.ac.jp
Nagamune
Hideaki
H
Maeda
Nobuko
N
D019881:Aminoacyltransferases; D001426:Bacterial Proteins / Q000235:genetics; D001430:Bacteriocins; D002352:Carrier Proteins / Q000235:genetics; D003603:Cytotoxins / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D005798:Genes, Bacterial; D020459:Genes, rRNA; D006602:Hexosyltransferases / Q000235:genetics; D008969:Molecular Sequence Data; D002267:Muramoylpentapeptide Carboxypeptidase / Q000235:genetics; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases / Q000235:genetics; D016133:Polymerase Chain Reaction; D011133:Polysaccharide-Lyases / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013291:Streptococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D034366:Streptococcus anginosus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D034365:Streptococcus constellatus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D034367:Streptococcus intermedius / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D001426:Bacterial Proteins; D001430:Bacteriocins; D002352:Carrier Proteins; D003603:Cytotoxins; D004269:DNA, Bacterial; D046915:Penicillin-Binding Proteins; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C403910:intermedilysin protein, Streptococcus intermedius; D019881:Aminoacyltransferases; C478701:penicillin-binding protein 2b, Streptococcus; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D011133:Polysaccharide-Lyases; C100002:hyaluronate lyase