D609 induces vascular endothelial cells and marrow stromal cells differentiation into neuron-like cells.
OBJECTIVE
To investigate the effect of tricyclodecane-9-yl-xanthogenate (D609) on cell differentiation in vascular endothelial cells (VECs) and marrow stromal cells (MSCs).


METHODS
Morphological changes were observed under phase contrast microscope. Electron microscope and immunostaining were used for VECs identification. The expressions of neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) were examined by immunohistochemistry.


RESULTS
After 6 h of induction with D609, some VECs showed morphological changes characteristic of neurones. 9 h later, more VECs became neuron-like cells. About 30.8% of VECs displayed positive NSE (P<0.01), while the expression of GFAP was negative. When MSCs were exposed to D609, the cells displayed neuronal morphologies, such as pyramidal cell bodies and processes formed extensive networks at 3 h. 6 h later, almost all of the cells exhibited a typical neuronal appearance, and 85.6% of MSCs displayed intensive positive NSE, but GFAP did not express.


CONCLUSIONS
D609 induces VECs and MSCs differentiation into neuron-like cells.
Wang
Nan
N
Institute of Developmental Biology, School of Life Science, Shandong University, Ji-nan 250100, China.
Du
Chun-qing
CQ
Wang
Shao-shan
SS
Xie
Kun
K
Zhang
Shang-li
SL
Miao
Jun-ying
JY
D000975:Antioxidants / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000166:cytology*; D001952:Bridged-Ring Compounds / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology*; D006801:Humans; D009474:Neurons / Q000166:cytology*; D009636:Norbornanes; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D017154:Stromal Cells / Q000166:cytology*; D013859:Thiocarbamates; D013871:Thiones / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology
D000975:Antioxidants; D001952:Bridged-Ring Compounds; D009636:Norbornanes; D010726:Phosphodiesterase Inhibitors; D013859:Thiocarbamates; D013871:Thiones; C046498:tricyclodecane-9-yl-xanthogenate