title: Differences among auxin treatments on haploid production in durum wheat x maize crosses.
abstract: Three doubled haploid lines of durum wheat [Triticum turgidum ssp. durum (Desf.) Husn.] were crossed with maize (Zea mays L.), and five hormone treatments were applied to test their effect on the production of caryopses, embryos and haploid plants. The auxin treatments consisted of 100 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), 5 mg/l or 50 mg/l dicamba and two combination mixtures of 95/5 mg/l and 50/50 mg/l 2,4-D plus dicamba, respectively. Hormones were added to the culture medium of the detached tillers. Differences were not observed among the four hormone treatments that contained dicamba, nevertheless, these treatments significantly increased the production of caryopses, embryos and haploid plants. On average, 8.9 caryopses, 2.6 embryos and 1.3 haploid plants per spike were obtained following the treatment with 100 mg/l 2,4-D, and 15.0 caryopses, 6.0 embryos and 3.0 haploid plants per spike were obtained following the various treatments with dicamba. We propose the application of dicamba alone, or dicamba plus 2,4-D, as a means for improving the yield of haploid plants of durum wheat through crosses with maize.
authors.affiliation: Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Apdo 4084, 14080, Cordoba, Spain.
authors.forename: C
authors.initials: C
authors.lastname: García-Llamas
authors.forename: A
authors.initials: A
authors.lastname: Martín
authors.forename: J
authors.initials: J
authors.lastname: Ballesteros
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D002678:Chimera / Q000235:genetics / Q000254:growth & development*; D003996:Dicamba / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D006238:Haploidy; D007210:Indoleacetic Acids / Q000494:pharmacology*; D030821:Plants, Genetically Modified / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D014908:Triticum / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D003313:Zea mays / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development
chemical_list: D007210:Indoleacetic Acids; D015084:2,4-Dichlorophenoxyacetic Acid; D003996:Dicamba