title: [Effect of long-term exposure to low dose gamma-irradiation on the rat thyroid status].
abstract: The effect of long-term exposure to low-dose external radiation on the rat thyroid status was studied. The experiments were carried out on Wistar female rats. The single doses absorbed were 0.1; 0.25; 0.5 Gy. The rats were irradiated 20 times (5 days x 4 weeks). The animals were decapitated after 1, 30 and 180 days following the last irradiation. Blood serum was assayed for content of thyroxin (T4) and triiodothyronine (T3) radioimmunologically. The liver was spectrophotometrically assayed for thyroid-induced NADP-malatedehydrogenase (NADP-MDH). It was shown that the long-term 0.5-Gy irradiation of the animals induced a decrease in blood T4 and T3 concentrations 1.34-1.71-fold and 1.24-1.43-fold after 1, 30 and 180 days, respectively. The T3 level was diminished most pronouncedly after 1 day, whereas that of T4--after 30 days following the exposure. With the doses of 0.1 and 0.25 Gy absorbed, the T4 and T3 concentration remained unchanged throughout all the periods studied. The activity of NADP-MDH was decreased 1.55-2.46-fold in all the experimental animals, and it was held decreased after 180 days (1.43-1.50-fold) in 0.25- and 0.5-Gy-irradiated groups, which indicates a disturbance in thyroid hormone metabolism in rats exposed chronically to low-dose radiation. After 180 days, the experimental animals experienced an elevation of thyroid gland weight on 15-20%. The thyroid status disturbance seemed to be due to both inhibited T4 and T3 biosynthesis in thyroid and disturbed hormone peripheral metabolism under radiation exposure.
authors.affiliation: Institute of Biochemistry, National Academy of Sciences of Belarus, Grodno, 230017 Belarus.
authors.forename: L I
authors.initials: LI
authors.lastname: Nadol'nik
authors.forename: Z V
authors.initials: ZV
authors.lastname: Netsetskaia
authors.forename: V V
authors.initials: VV
authors.lastname: Vinogradov
mesh_terms: D000818:Animals; D005260:Female; D008099:Liver / Q000201:enzymology / Q000528:radiation effects; D008291:Malate Dehydrogenase / Q000032:analysis; D050538:Malate Dehydrogenase (NADP+); D011829:Radiation Dosage; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D013053:Spectrophotometry; D013961:Thyroid Gland / Q000378:metabolism / Q000528:radiation effects*; D013974:Thyroxine / Q000096:biosynthesis / Q000097:blood; D013997:Time Factors; D014284:Triiodothyronine / Q000096:biosynthesis / Q000097:blood
chemical_list: D014284:Triiodothyronine; D008291:Malate Dehydrogenase; D050538:Malate Dehydrogenase (NADP+); D013974:Thyroxine