Design and evaluation of a specific primer pair for the diagnosis and identification of Acanthamoeba polyphaga.
Random amplified polymorphism DNA (RAPD) is a useful tool for species identification. The obtained band patterns can be used for specific primer pair design that may be useful for species diagnosis. In this study, a distinctive a 962-bp band in A. polyphaga band patterns was found, by using the OPC20 primer (ACTTCGCCAC). The DNA fragment was used to design a specific primer pair that was useful for the identification of different isolates as A. polyphaga species. A case of A. polyphaga in disseminated acanthamoebiasis affecting mesenteric nodes is also reported.
Ortega-Rivas
Antonio
A
Department of Parasitology, Faculty of Pharmacy, University of La Laguna, Avda. Francisco Sánchez s/n, C.P. 38271, La Laguna, Tenerife, Spain. aortega@ull.es
Lorenzo-Morales
Jacob
J
Martinez-Carretero
Enrique
E
Villa Lloberas
Mercedes
M
Valladares Hernández
Basilio
B
del Castillo-Remiro
Antonio
A
D000048:Acanthamoeba / Q000235:genetics* / Q000302:isolation & purification*; D000562:Amebiasis / Q000175:diagnosis* / Q000209:etiology; D000818:Animals; D016172:DNA Fingerprinting; D017931:DNA Primers; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D008198:Lymph Nodes / Q000469:parasitology; D008643:Mesentery / Q000469:parasitology; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D012680:Sensitivity and Specificity
D017931:DNA Primers; D015342:DNA Probes; D016054:DNA, Protozoan