Upregulation of the Hsp104 chaperone at physiological temperature during recovery from thermal insult.
Thermal insult at 50 degrees C causes protein denaturation in yeast, but the cells survive if preconditioned at 37 degrees C. Survival depends on refolding of heat-denatured proteins. Refolding of cytoplasmic proteins requires Hsp104, the expression of which increases several-fold upon shift of the cells from physiological temperature 24 degrees C to 37 degrees C. We describe here a novel type of regulation of Hsp104, designated delayed upregulation (DUR). When Saccharomyces cerevisiae cells grown at 24 degrees C, preconditioned at 37 degrees C and treated briefly at 50 degrees C were shifted back to 24 degrees C, Hsp104 expression was negligible for 1 h, but increased then to a three to nine times higher level than that detected after growth at 24 degrees C, returning to normal after 5 h. A heat shock element (HSE) of the upstream sequence of HSP104 was necessary and sufficient for DUR, whereas stress response elements (STRE) were dispensable. Destruction of HSE plus all three STREs abolished Hsp104 expression, resulting in cell death after thermal insult. Deletion of MSN2/4, encoding transcription factors driving STRE-dependent gene expression, decreased DUR. Deletion of HOG1, encoding a heat-responsive and osmosensitive mitogen-activated protein kinase implicated to be functionally connected to Msn2/4p, abolished DUR. We suggest that DUR was regulated via HSE, required Hog1p and involved Msn2/4p-regulated gene products.
Seppä
Laura
L
Program in Cellular Biotechnology, Institute of Biotechnology, University of Helsinki, Viikinkaari 9, 00710 Helsinki, Finland.
Hänninen
Anna-Liisa
AL
Makarow
Marja
M
D015169:Colony Count, Microbial; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D018869:Heat-Shock Response / Q000235:genetics*; D006358:Hot Temperature; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000502:physiology; D009154:Mutation; D011401:Promoter Regions, Genetic; D017510:Protein Folding; D012333:RNA, Messenger / Q000032:analysis; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology; D015854:Up-Regulation / Q000235:genetics / Q000502:physiology
D004268:DNA-Binding Proteins; D006360:Heat-Shock Proteins; C081935:MSN2 protein, S cerevisiae; C081937:MSN4 protein, S cerevisiae; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C070972:HsP104 protein, S cerevisiae; C088589:HOG1 protein, S cerevisiae; D020928:Mitogen-Activated Protein Kinases