Cloning and characterization of a novel human cDNA encoding a J-domain protein (DNAJA5) from the fetal brain.
J-domain proteins are involved in protein importing and sorting, protein translocation into the endoplasmic reticulum and interaction with hsp70, cell cycle regulation and exocytosis. During the large-scale sequencing analysis of a human fetal brain cDNA library, we isolated a cDNA clone encoding a putative 531 amino acid protein with a DnaJ domain and C2H2-type zinc finger motif, which was named as DNAJA5 (human DnaJ homology subfamily A member 5). The putative protein of DNAJA5 gene was homologous to Caenorhabditis elegans DNJ-17 (identity 33.2% similarity 45.1%). By mapping, we located DNAJA5 gene to human chromosome 5p12-5p13. The DNAJA5 gene contained at least 12 exons that spanned more than 25.6 kb of the genomic sequence. The reverse transcription-polymerase chain reaction analysis indicated that it was expressed in the human brain, placenta, kidney and pancreas. DNAJA5 might play a role in co-operating with Hsp70.
Chen
Juxiang
J
Department of Neurosurgery, ChangZheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China.
Yin
Gang
G
Lu
Yicheng
Y
Lou
Meiqing
M
Cheng
Haipeng
H
Ni
Xiaohua
X
Hu
Guohan
G
Luo
Chun
C
Ying
Kang
K
Xie
Yi
Y
Mao
Yumin
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D005091:Exons / Q000235:genetics; D005333:Fetus / Q000378:metabolism*; D020869:Gene Expression Profiling; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D007438:Introns / Q000235:genetics; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism; D020161:Physical Chromosome Mapping; D010920:Placenta / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins