Effect of amoxicillin-clavulanic acid on human fecal flora in a gnotobiotic mouse model assessed with fluorescence hybridization using group-specific 16S rRNA probes in combination with flow cytometry.
Predominant groups of bacteria from a human fecal flora-associated mouse model challenged with amoxicillin-clavulanic acid were quantified with fluorescence in situ hybridization combined with flow cytometry using specific 16S rRNA targeted oligonucleotide probes. This approach provides a useful tool with high throughput to evaluate fecal microflora under antibiotic treatment.
Barc
Marie Claude
MC
Unité associée INRA Ecosystème Microbien Digestif et Santé, Faculté de Pharmacie, Université Paris-Sud, 92296 Châtenay-Malabry Cedex, France.
Bourlioux
François
F
Rigottier-Gois
Lionel
L
Charrin-Sarnel
Céline
C
Janoir
Claire
C
Boureau
Hélène
H
Doré
Joël
J
Collignon
Anne
A
D019980:Amoxicillin-Potassium Clavulanate Combination / Q000494:pharmacology*; D000818:Animals; D001421:Bacteria, Anaerobic / Q000187:drug effects; D015169:Colony Count, Microbial; D004359:Drug Therapy, Combination / Q000494:pharmacology*; D005243:Feces / Q000382:microbiology*; D005260:Female; D005434:Flow Cytometry; D005856:Germ-Free Life; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008809:Mice, Inbred C3H; D015345:Oligonucleotide Probes; D015347:RNA Probes; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
D015345:Oligonucleotide Probes; D015347:RNA Probes; D012336:RNA, Ribosomal, 16S; D019980:Amoxicillin-Potassium Clavulanate Combination