Periodontal repair in surgically created intrabony defects in dogs: influence of the number of bone walls on healing response.
BACKGROUND
The objective of this study was to histologically evaluate periodontal healing following flap surgery in intrabony periodontal defects to determine the influence of the number of bone walls on periodontal regeneration.


METHODS
One-, 2-, and 3-wall intrabony periodontal defects were surgically produced at the proximal aspect of mandibular premolars in either right or left jaw quadrants in six beagle dogs. Mucoperiosteal flaps were positioned and sutured to their presurgery position following defect preparation. The animals were euthanized at 8 weeks post-surgery, and block sections of the defect sites were collected for histologic and histometric analysis.


RESULTS
Bone and cementum regeneration was positively correlated to the number of bone walls limiting the intrabony periodontal defects. The junctional epithelium averaged (+/- SD) 1.5 +/- 0.2, 1.2 +/- 0.3, and 0.9 +/- 0.2 mm for the 1-, 2-, and 3-wall defects, respectively, with the 3-wall defects being significantly different from the 1-wall defects (P <0.05). Cementum regeneration averaged 1.2 +/- 0.6, 2.0 +/- 0.6, and 2.8 +/- 0.5 mm for the 1-, 2-, and 3-wall defects, respectively; all groups were significantly different from each other (P <0.05). Bone regeneration averaged 1.5 +/- 0.5, 1.7 +/- 0.6, and 2.3 +/- 0.5 mm for the 1-, 2-, and 3-wall defects, respectively, with the 3-wall defects being significantly different from the 1-wall defects (P <0.05).


CONCLUSIONS
The results suggest that the number of bone walls is a critical factor determining treatment outcomes in intrabony periodontal defects. One- and 3-wall intrabony defects appear to be reproducible models to evaluate candidate technologies for periodontal regeneration.
Kim
Chang-Sung
CS
Department of Periodontology, Research Institute for Periodontal Regeneration, Oral Science Research Center, College of Dentistry, Yonsei University, Seoul, Korea.
Choi
Seong-Ho
SH
Chai
Jung-Kiu
JK
Cho
Kyoo-Sung
KS
Moon
Ik-Sang
IS
Wikesjö
Ulf M E
UM
Kim
Chong-Kwan
CK
D016301:Alveolar Bone Loss / Q000473:pathology* / Q000601:surgery; D000539:Alveolar Process / Q000473:pathology; D000541:Alveolectomy; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology; D003739:Dental Cementum / Q000473:pathology; D004285:Dogs; D004846:Epithelial Attachment / Q000473:pathology; D008334:Mandible / Q000473:pathology / Q000601:surgery; D012038:Regeneration / Q000502:physiology; D013524:Surgical Flaps / Q000473:pathology; D019237:Tooth Cervix / Q000473:pathology; D014092:Tooth Root / Q000473:pathology; D016896:Treatment Outcome; D014945:Wound Healing / Q000502:physiology