Evidence of metyrapone reduction by two Mycobacterium strains shown by 1H NMR.
In situ 1H NMR monitoring of metyrapone incubations with resting-cells of two strains of Mycobacterium, Mycobacterium aurum MO1 and Mycobacterium sp. RP1, showed the biotransformation of this compound, and more precisely the carbonyl-reduction of metyrapone into the corresponding alcohol, metyrapol. This reduction produced both enantiomers. The use of inhibitors allowed us to show the multiple enzymatic activities involved in this biotransformation including carbonyl reductase (EC 1.1.1.1.84) from the short-chain dehydrogenase superfamily and aldehyde reductase (EC 1.1.1.2) from the aldo-keto reductase superfamily.
Combourieu
Bruno
B
Laboratoire de Synthèse et Etude de Systèmes à Intérêt Biologique, UMR 6504 du CNRS, Université, Blaise Pascal, 63177 Aubière Cedex, France.
Besse
Pascale
P
Sancelme
Martine
M
Maser
Edmund
E
Delort
Anne-Marie
AM
D001673:Biodegradation, Environmental; D003470:Culture Media; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008797:Metyrapone / Q000378:metabolism*; D009161:Mycobacterium / Q000254:growth & development / Q000378:metabolism*; D010084:Oxidation-Reduction
D003470:Culture Media; D008797:Metyrapone