title: Immunohistochemical labelling of cytokines in lung lesions of pigs naturally infected with Mycoplasma hyopneumoniae.
abstract: Mycoplasma hyopneumoniae (Mh) is the primary agent of porcine enzootic pneumonia (PEN), a chronic respiratory disease endemic to pig farms, and characterized histologically by infiltration of mononuclear cells in airways and prominent hyperplasia of the bronchus-associated lymphoid tissue (BALT). To gain further insight into the pathogenesis of PEN, cytokine expression in the lung, with particular attention to the BALT, was examined immunohistochemically in pigs naturally infected with Mh. An increase (P < 0.05) in proinflammatory and immunoregulatory cytokines (especially interleukin [IL]-2, IL-4 and tumour necrosis factor [TNF]-alpha, and to a lesser extent IL-1 [alpha and beta] and IL-6) was detected in the BALT, which showed intense lymphoid hyperplasia. IL-1beta and TNF-alpha were also detected in the bronchoalveolar exudate of infected pigs, and IL-6 and IL-8 were demonstrated in mononuclear cells of the alveolar septa. The results showed that in Mh infection, macrophage and lymphocyte activation results in the expression of a number of cytokines capable of inducing lung lesions and lymphoreticular hyperplasia of the BALT.
authors.affiliation: Department of Comparative Pathology, Veterinary Faculty, University of Las Palmas de Gran Canaria, Trasmontaña s/n, 35416 Arucas, Gran Canaria, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Rodríguez
authors.forename: G A
authors.initials: GA
authors.lastname: Ramírez
authors.forename: J
authors.initials: J
authors.lastname: Sarradell
authors.forename: M
authors.initials: M
authors.lastname: Andrada
authors.forename: H
authors.initials: H
authors.lastname: Lorenzo
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung / Q000276:immunology / Q000378:metabolism* / Q000473:pathology*; D008214:Lymphocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D045705:Mycoplasma hyopneumoniae / Q000276:immunology; D045729:Pneumonia of Swine, Mycoplasmal / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000378:metabolism* / Q000473:pathology
chemical_list: D016207:Cytokines