title: Whole blood assay to access T cell-immune responses to Mycobacterium tuberculosis antigens in healthy Brazilian individuals.
abstract: The production of interferon gamma (IFNgamma) guarantees effective T cell-mediated immunity against Mycobacterium tuberculosis infection. In the present study, we simply compare the in vitro immune responses to Mycobacterium antigens in terms of IFNg production in a total of 10 healthy Brazilian volunteers. Whole blood and mononuclear cells were cultivated in parallel with PPD, Ag85B, and M. bovis hsp65, and five-days supernatants were harvested for cytokine detection by ELISA. The inter-assay result was that the overall profile of agreement in response to antigens was highly correlated (r(2) = 0.9266; p = 0.0102). Potential analysis is in current progress to dictate the usefulness of this method to access the immune responses also in tuberculosis patients and its contacts.
authors.affiliation: Laboratório de Hanseníase, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, 21045-900, Brasil.
authors.forename: Paulo R Z
authors.initials: PR
authors.lastname: Antas
authors.forename: Fernando L L
authors.initials: FL
authors.lastname: Cardoso
authors.forename: Eliane B
authors.initials: EB
authors.lastname: Oliveira
authors.forename: Patrícia K C
authors.initials: PK
authors.lastname: Gomes
authors.forename: Kátia S
authors.initials: KS
authors.lastname: Cunha
authors.forename: Euzenir N
authors.initials: EN
authors.lastname: Sarno
authors.forename: Elizabeth P
authors.initials: EP
authors.lastname: Sampaio
mesh_terms: D000328:Adult; D000942:Antigens, Bacterial / Q000097:blood / Q000276:immunology*; D018929:Cell Culture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000097:blood; D008297:Male; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014374:Tuberculin Test / Q000379:methods; D014376:Tuberculosis / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; C072681:Mycobacterium tuberculosis antigens; D007371:Interferon-gamma