title: Sporicidal efficacy of hydrogen peroxide aerosol.
abstract: Because of its high sporicidal activity but low toxicity, hydrogen peroxide (H2O2) is a useful agent for gentle antimicrobial treatment of irritable goods. Above all to optimise practical aspects of H2O2 application, sporicidal efficacy of aerosols based on 1% H2O2 solution was tested. Using commercially available bioindicators with Bacillus subtilis spores immobilized on filter paper strips, a 7 h lasting aerosol treatment at room temperature resulted in a reduction of viable count of 2.5 log10 (median, n = 5). By combined application of 0.3% sorbitan monolaurate (Span 20) and 1% H2O2 aerosol at room temperature, a reduction of viable count of >6.3 log10 (median, n = 5) could be reached within 2 h, already. Following aerosol treatment at 60 degrees C, all bioindicators tested (n = 15) have been found without growth after 10 min, corresponding to a reduction of viable count >6.3 log10. However, this efficacy was possible only if the spores were immobilized on filter paper strips. Changing the carrier materials lower sporicidal activity of H2O2 aerosol was found resulting in a reduction of viable count between >5.4 log10 (median, n = 15) on polyethylene foil and 0.4 log10 (median, n = 15) on glass slides. These differences could not be correlated with surface qualities as porosity or wettability.
authors.affiliation: Institute of Pharmacy, Ernst Moritz Arndt University, Greifswald, Germany. woedtke@biosensoren.de
authors.forename: Th
authors.initials: T
authors.lastname: von Woedtke
authors.forename: K
authors.initials: K
authors.lastname: Haese
authors.forename: J
authors.initials: J
authors.lastname: Heinze
authors.forename: Ch
authors.initials: Ch
authors.lastname: Oloff
authors.forename: M
authors.initials: M
authors.lastname: Stieber
authors.forename: W D
authors.initials: WD
authors.lastname: Jülich
mesh_terms: D000336:Aerosols; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage* / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects; D005079:Excipients; D006861:Hydrogen Peroxide / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology*; D013012:Sorbitol; D013171:Spores, Bacterial / Q000187:drug effects*; D013499:Surface Properties
chemical_list: D000336:Aerosols; D000891:Anti-Infective Agents, Local; D005079:Excipients; D013012:Sorbitol; D006861:Hydrogen Peroxide