title: CITED1 protein expression suggests Papillary Thyroid Carcinoma in high throughput tissue microarray-based study.
abstract: Molecular markers of papillary thyroid carcinoma (PTC) are relatively unknown. Recently, the CITED1 gene was reported to be greatly upregulated in PTC relative to normal thyroid. The CITED1 protein, a 27-kd transcriptional transactivator nuclear protein is expressed in PTC, melanocytes, breast epithelial cells, and several embryonic tissues. However, its expression in other thyroid masses and non-thyroid tumors is not known. We evaluated CITED1 protein expression in tissue microarrays comprising various thyroid and nonthyroid tissues by immunohistochemistry using a polyclonal anti-CITED1 antibody. CITED1 expression was seen in 63 of 68 PTC (93%), 3 of 12 follicular carcinomas (25%), 2 of 7 Hürthle cell carcinomas (28%), 2 of 21 adenomas (10%), 2 of 6 follicular neoplasms of undetermined malignant behavior (33%), and 2 of 24 nodular goiters (8%). Normal thyroids (n = 27), thyrotoxic hyperplasias (n = 14), and anaplastic thyroid carcinomas (n = 5) did not express CITED1. Among nonthyroid tumors, 6 of 23 melanomas (26%), 11 of 65 prostatic carcinomas (17%), 3 of 25 glioblastomas (12%), 4 of 67 breast carcinomas (6%), 1 of 49 lymphomas (2%), 1 of 65 lung carcinomas (2%), 1 of 68 colon carcinomas (2%), and none of 49 ovarian carcinomas (0%) expressed CITED1. The accuracy of CITED1 in differentiating PTC from benign thyroid nodules, other thyroid carcinomas, and nonthyroid carcinomas was 93%, 89%, and 94%, respectively. CITED1 is preferentially expressed in PTC and may be used as a diagnostic marker of it.
authors.affiliation: Department of Pathology, Ohio State University, Columbus, Ohio 43210, USA. Prasad-l@medctr.osu.edu
authors.forename: Manju L
authors.initials: ML
authors.lastname: Prasad
authors.forename: Natalia S
authors.initials: NS
authors.lastname: Pellegata
authors.forename: Richard T
authors.initials: RT
authors.lastname: Kloos
authors.forename: Catalin
authors.initials: C
authors.lastname: Barbacioru
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Albert
authors.initials: A
authors.lastname: de la Chapelle
mesh_terms: D018263:Adenocarcinoma, Follicular / Q000378:metabolism; D000236:Adenoma / Q000378:metabolism; D018249:Adenoma, Oxyphilic / Q000378:metabolism; D051017:Apoptosis Regulatory Proteins; D002277:Carcinoma / Q000175:diagnosis / Q000378:metabolism; D002291:Carcinoma, Papillary / Q000175:diagnosis* / Q000378:metabolism*; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry; D009687:Nuclear Proteins; D020411:Oligonucleotide Array Sequence Analysis; D012680:Sensitivity and Specificity; D013964:Thyroid Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D016606:Thyroid Nodule / Q000175:diagnosis / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors
chemical_list: D051017:Apoptosis Regulatory Proteins; C512688:CITED1 protein, human; D009687:Nuclear Proteins; D015534:Trans-Activators; D014157:Transcription Factors