Halobacillus locisalis sp. nov., a halophilic bacterium isolated from a marine solar saltern of the Yellow Sea in Korea.
A Gram-positive, motile, endospore-forming and rod-shaped halophilic bacterial strain MSS-155 (KCTC 3788 and KCCM 41687) was isolated from a marine solar saltern of the Yellow Sea in Korea and was subjected to a polyphasic taxonomic study. This organism grew at temperature of 10.0-42.0 degrees C with an optimum of 35 degrees C. Strain MSS-155 grew optimally in the presence of 10% NaCl and did not grow in the absence of NaCl. The cell wall peptidoglycan type of strain MSS-155 was A4beta based on l-Orn- d-Asp. Strain MSS-155 was also characterized chemotaxonomically by having menaquinone-7 (MK-7) as the predominant isoprenoid quinone and anteiso-C15:0 as the major fatty acid. The DNA G + C content was 44.0 mol%. Phylogenetic analysis based on 16S rDNA sequences showed that strain MSS-155 falls within the radiation of the cluster comprising Halobacillus species. Levels of 16S rDNA sequence similarity between strain MSS-155 and the type strains of four Halobacillus species were in the range 97.6-98.8%. Strain MSS-155 exhibited levels of DNA-DNA relatedness of 6.2-11.2% to the type strains of Halobacillus species described previously. On the basis of phenotypic properties, phylogeny, and genomic data, strain MSS-155 should be placed in the genus Halobacillus as a member of a novel species, for which we propose the name Halobacillus locisalis sp. nov.
Yoon
Jung-Hoon
JH
Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yusong, Taejon, Korea, jhyoon@kribb.re.kr
Kang
Kook Hee
KH
Oh
Tae-Kwang
TK
Park
Yong-Ha
YH
D001406:Bacillaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001482:Base Composition; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D007723:Korea; D010641:Phenotype; D010802:Phylogeny; D012623:Seawater / Q000382:microbiology; D012965:Sodium Chloride / Q000378:metabolism
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012965:Sodium Chloride