title: PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR.
abstract: PerlPrimer is a cross-platform graphical user interface application for the design of primers for standard, bisulphite and real-time PCR, and sequencing. The program incorporates highly accurate melting-temperature and primer-dimer prediction algorithms with powerful tools such as sequence retrieval from Ensembl and the ability to BLAST search primer pairs. It aims to automate and simplify the process of primer design.
  
  
  BACKGROUND
  Open-source and freely available from http://perlprimer.sourceforge.net.
authors.affiliation: Murdoch Childrens Research Institute, Royal Childrens Hospital, Flemington Road, Parkville, Vic., 3052, Australia. owen.marshall@mcri.edu.au
authors.forename: Owen J
authors.initials: OJ
authors.lastname: Marshall
mesh_terms: D000465:Algorithms; D002874:Chromosome Mapping / Q000379:methods*; D017931:DNA Primers / Q000138:chemical synthesis / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D009862:Online Systems; D016133:Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software; D013447:Sulfites; D014584:User-Computer Interface
chemical_list: D017931:DNA Primers; D013447:Sulfites