[Acute cell rejection in ex vivo model of swine-human renal xenotransplantation].
OBJECTIVE
Ex vivo perfusion model of pig-to-human kidney xenotransplantation to evaluate human antiporcine xenograft rejection once hyperacute rejection (HAR) is avoided.


METHODS
Pig kidneys were perfused for 3 hours with pig blood (group 1; n = 5), human blood (group 2; n = 5), complement heat inactivated human blood (group 3; n = 5), platelet-depleted human blood (group 4; n = 5); and xenoreactive natural antibodies (XNA)-depleted human blood (group 5; n = 5). Tissue samples were studied with immunoperoxidase techniques.


RESULTS
Pig kidneys perfused with human blood, group 2, showed HAR with interstitial haemorrhage, vascular thrombi and glomerular injury. Pig kidneys perfused with manipulated human blood (groups 3 to 5) had no histologic evidence of HAR, but showed signs of acute cellular rejection with different degrees of interstitial infiltrate of mononuclear cells, specially in the XNA-depleted group.


CONCLUSIONS
The model may be valuable in the isolated evaluation of the elements involved in the pig-to-human xenorejection. The depletion of complement, platelets and XNA protected porcine kidneys form HAR in our study and allowed the development of an acute cellular rejection, a very unusual fact in this short time, 3 hours.
Vega Vega
A
A
Servicio de Urología de la Obra Hospitalaria de Ntra Sra de Regla, León.
García Alonso
D
D
Ramos Barrón
A
A
Val Bernal
F
F
Pino Chávez
G
G
Gómez Fleitas
M
M
Arias Rodríguez
M
M
D000208:Acute Disease; D000818:Animals; D006084:Graft Rejection / Q000276:immunology; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology / Q000379:methods; D013552:Swine; D014183:Transplantation, Heterologous