Tumor-suppressive activities by chemokines introduced into OV-HM cells using fiber-mutant adenovirus vectors.
In this study, fiber-mutant adenovirus vectors encoding chemokines, Ad-RGD-mCCL17, Ad-RGD-mCCL21 and Ad-RGD-mCCL22 were constructed. The insertion of integrin-targeting RGD sequence into fiber knob of adenovirus vectors notably enhanced the infection efficiency into tumor cells. Among three chemokine-encoding vectors evaluated, Ad-RGD-mCCL22 showed significant tumor-suppressive activity via transduction into OV-HM cells.
Gao
J Q
JQ
Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita City, Japan.
Alexandre
L S
LS
Tsuda
Y
Y
Katayama
K
K
Eto
Y
Y
Sekiguchi
F
F
Mizuguchi
H
H
Hayakawa
T
T
Nakayama
T
T
Yoshie
O
O
Tsutsumi
Y
Y
Mayumi
T
T
Nakagawa
S
S
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D018925:Chemokines / Q000235:genetics* / Q000502:physiology*; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D051379:Mice; D009154:Mutation / Q000235:genetics; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000188:drug therapy; D014407:Tumor Cells, Cultured
D018925:Chemokines