The design of a novel complementary metal oxide semiconductor detection system for biochemical luminescence.
We designed a complementary metal oxide semiconductor (CMOS) chip with accompanied accessories as a system for the detections and quantifications of biochemical luminescence. This is the first of such instruments that has been reported. The semiconductor chip was manufactured through a 0.25 microm CMOS standard process. A current mirror was designed in integrated circuit (IC) to amplify the signal current that was induced by chemiluminescence. Horseradish peroxidase (HRP)-luminol-H2O2 system was used as an example to constitute a useful platform for coupling to chemiluminescence reactions which produce H2O2. Glucose-glucose oxidase (GOD) reaction was coupled with HRP-luminol-H2O2 reaction to demonstrate the ability of the novel CMOS base instrument for quantifying the biological luminescence of a variety of valuable clinical assays. Our results illustrated that the combination of the specifically designed CMOS IC and commercially available electronic devices established a simple and useful bioanalytical tool.
Lu
Ude
U
Department of Biological Science and Technology, National Chiao Tung University, Institute of Biochemical Engineering, 75 Po-Ai Street, Hsinchu, Taiwan.
Hu
Ben C-P
BC
Shih
Yu-Chuan
YC
Wu
Chung-Yu
CY
Yang
Yuh-Shyong
YS
D015374:Biosensing Techniques / Q000295:instrumentation*; D005947:Glucose / Q000032:analysis; D006861:Hydrogen Peroxide / Q000032:analysis; D007700:Kinetics; D008163:Luminescent Measurements; D010087:Oxides; D012666:Semiconductors; D013997:Time Factors
D010087:Oxides; D006861:Hydrogen Peroxide; D005947:Glucose