Biomphalaria tenagophila: genetic variability within intermediate snail hosts susceptible and resistant to Schistosoma mansoni infection.
DNA analysis by molecular techniques has significantly expanded the perspectives of the study and understanding of genetic variability in molluscs that are vectors of schistosomiasis. In the present study, the genetic variability of susceptible and resistant B. lenagophila strains to S. mansoni infection was investigated using amplification of their genomic DNA by RAPD-PCR. The products were analyzed by PAGE and stained with silver. The results showed polymorphism between tested strains with four different primers. We found two bands of 1,900 and 3,420 bp that were characteristic of the susceptible strains with primer 2. The primers 9 and 10 identified a single polymorphic band that was also characteristic of (3,136 and 5,041 bp, respectively) susceptible snails. Two polymorphic bands were detected by primer 15: one with 1,800 bp was characteristic of the resistant strain and the other with approximately equal to 1,700 bp in the susceptible one. These results provide additional evidence showing that the RAPD-PCR technique is adequate for the study of polymorphisms in intermediate hosts snails of S. mansoni. The obtained results are expected to expand the knowledge about the genetic variability of the snails and to permit the future identification of genomic sequences specifically related to the resistance/susceptibility of Biomphalaria to the larval forms of S. mansoni.
Da Silva
D
D
Department of Biological Sciences, UNESP, Av. Dom Antonio, 2100, Assis, SP, Brazil, 19806-900.
Spada
R G
RG
Sobral-Hamaguchi
S S
SS
Abdel-Hamid
Z
Z
Zuim
N R
NR
Zanotti-Magalhães
E M
EM
Magalhães
L A
LA
Ribeiro-Paes
J T
JT
D000818:Animals; D001700:Biomphalaria / Q000235:genetics* / Q000469:parasitology*; D018509:DNA, Helminth / Q000032:analysis / Q000235:genetics; D004199:Disease Vectors; D014644:Genetic Variation; D006790:Host-Parasite Interactions; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods / Q000662:veterinary*; D012550:Schistosoma mansoni / Q000502:physiology*
D018509:DNA, Helminth