Orangutan herpesvirus.
A male orangutan suffered from ulcers at the buccal mucosa. We obtained swab fluid from the base of both vesicles and ulcers and collected blood for further separation into serum, plasma and peripheral blood mononuclear cells (PBMC) for detection of antibody to herpesvirus by serology and herpesvirus DNA by polymerase chain reaction (PCR) using consensus degenerate primers. Serology was positive for human EBV IgG but negative for Epstein-Barr virus (EBV) immunoglobulin (IgM), as well as for both human cytomegalovirus and herpes simplex virus IgG and IgM. Upon PCR, we obtained a 232-bp product of virus DNA from PBMC, but not from lesions, serum or plasma. We confirmed the positive result by direct sequencing and compared the nucleotide sequence with other nucleotide sequences applying the BLAST program from GenBank. The sequence was similar to lymphocryptovirus of macaque (93%), marmoset (93%), gorilla (90%) and human EBV (90%). We aligned this sequence with other sequences in GenBank and performed phylogenetic analysis, showing that it probably belongs to the gammaherpesvirus group.
Sakulwira
Kamol
K
Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
Theamboonlers
Apiradee
A
Oraveerakul
Kanisak
K
Chaiyabutr
Narongsak
N
Bhattarakosol
Parvapan
P
Poovorawan
Yong
Y
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D018420:Ape Diseases / Q000473:pathology / Q000821:virology*; D001483:Base Sequence; D017931:DNA Primers; D018142:Gammaherpesvirinae / Q000235:genetics* / Q000276:immunology*; D006566:Herpesviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D004854:Herpesvirus 4, Human / Q000276:immunology; D007074:Immunoglobulin G; D007963:Leukocytes, Mononuclear / Q000821:virology; D008297:Male; D008969:Molecular Sequence Data; D009061:Mouth Mucosa / Q000473:pathology / Q000821:virology; D010802:Phylogeny; D011148:Pongo pygmaeus; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D000914:Antibodies, Viral; D017931:DNA Primers; D007074:Immunoglobulin G