Real-time PCR analysis of carbon catabolite repression of cellobiose dehydrogenase gene transcription in Trametes versicolor.
Cellobiose dehydrogenase production in Trametes versicolor is repressed when additional carbon sources, such as glucose, maltose, galactose, arabinose, and xylose, are added to the fungal cultures growing on cellulose. Real-time quantitative reverse transcription-polymerase chain reaction has been used to demonstrate that the addition of galactose, arabinose, and xylose results in 19-, 92-, and 114-fold reductions, respectively, in cdh transcript levels 96 h post-addition. Glucose exhibits the greatest repressive effect, resulting in a 3400-fold decrease in cdh transcript levels.
Stapleton
P C
PC
Microbiology Department, University College Cork, National University of Ireland, Cork, Ireland, UK.
O'Mahony
J
J
Dobson
A D W
AD
D001089:Arabinose / Q000378:metabolism; D002237:Carbohydrate Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D050260:Carbohydrate Metabolism; D004794:Enzyme Repression / Q000235:genetics / Q000502:physiology; D005690:Galactose / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000235:genetics* / Q000502:physiology; D005947:Glucose / Q000378:metabolism; D008320:Maltose / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D020072:Polyporales / Q000201:enzymology* / Q000235:genetics*; D012331:RNA, Fungal / Q000032:analysis / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis / Q000302:isolation & purification; D014158:Transcription, Genetic; D014994:Xylose / Q000378:metabolism
D012331:RNA, Fungal; D012333:RNA, Messenger; D008320:Maltose; D014994:Xylose; D001089:Arabinose; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase; D005947:Glucose; D005690:Galactose