[The experimental study of guinea pig cytomegalovirus infection in the kidney of the pup of guinea pig].
OBJECTIVE
To study the relationship of guinea pig cytomegalovirus (GPCMV) infection with the outcome of pregnancy by the kidney of guinea pig (GP).


METHODS
Twenty first-trimester gestation GPs were randomly selected, intraperitoneally inoculated with GPCMV. Then female GPs and the pups were killed within 24 h after delivery. By in situ hybridization (ISH) with three phases GPCMV late-mRNA probes labeled by digoxin, the virus load and its distribution were screened inside the pup's kidney.


RESULTS
Twenty GPs totally conceived 63 pups. Among them, 42 had normal outcome and lived longer than 24 h; 21 had abnormal outcome such as abortion, fetal death, et al. By in situ hybridization, the infection rate of normal pups was 7.1% (3/42) and the average optical density (A) was 0.105 +/- 0.052. The infection rate of abnormal pups was 28. 6% (6/21) and the A was 0.158 +/- 0.047. The difference of the A was significant (t = 2.57, P < 0.05). The positive signal of ISH was mainly distributed in the epithelium of renal tubule and collecting duct.


CONCLUSIONS
It is concluded that the late-mRNA mainly expressed in the epithelium of renal tubule and collecting duct and the expression level was related with the abnormal pregnancy outcome.
Wang
Xin-rong
XR
Department of Obstetrics and Gynecology, Tongji Hospital, Huazhong Science and Technology University, Wuhan 430030, China.
Chen
Su-hua
SH
Liu
Hai-zhi
HZ
Xiong
Jin-wen
JW
Ling
Xia-zhen
XZ
D000818:Animals; D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000150:complications* / Q000382:microbiology; D004195:Disease Models, Animal; D005260:Female; D006168:Guinea Pigs; D017403:In Situ Hybridization; D007668:Kidney / Q000473:pathology / Q000821:virology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious; D011256:Pregnancy Outcome; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation
D012333:RNA, Messenger