title: Visualization of micropatterned complex biosensor sensing chemistries by means of scanning electrochemical microscopy.
abstract: Redox hydrogel-based micropatterned complex biosensor architectures, used as sensing chemistries in amperometric ethanol or glucose biosensors, were deposited on gold, graphite or glass. Well-localized immobilization of active hydrogels with variable compositions was achieved by dispensing 100 pl droplets of cocktails containing alcohol or glucose dehydrogenase, redox polymer (PVI(13)dmeOs) and crosslinker (PEGDGE) while moving the target surface relative to the position of the nozzle of a piezo-actuated microdispenser. The resulting structures were microscopic patterns of enzyme-containing lines of a redox hydrogel with a line width of about 100 microm. Scanning electrochemical microscopy (SECM) in the amperometric feedback mode was used to visualize the immobilized enzyme microstructures and their localized biochemical activity was observed with high lateral resolution by detecting the enzymatically consumed substrate using K(4)[Fe(CN)(6)] as a free-diffusing electron-transfer mediator.
authors.affiliation: Department of Biotechnology, Lund University, P.O. Box 124, S-22100 Lund, Sweden.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Niculescu
authors.forename: Szilveszter
authors.initials: S
authors.lastname: Gáspár
authors.forename: Albert
authors.initials: A
authors.lastname: Schulte
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Csöregi
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schuhmann
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D004798:Enzymes; D020100:Hydrogels; D010084:Oxidation-Reduction
chemical_list: D004798:Enzymes; D020100:Hydrogels