In vivo immunomodulation by Mycoplasma fermentans membrane lipoprotein.
The immunomodulatory effects of Mycoplasma fermentans-derived membrane lipoprotein (LAMPf) in BALB/c mice were examined. When injected intraperitoneally into mice, LAMPf induced a transitory splenomegaly followed by a suppression of the spleen cell proliferation in response to concanavalin A, whereas responses to lipopolysaccharide and to LAMPf were unchanged. The intravenous injection of a large dose of LAMPf induced leukopenia and granulocyte-macrophage colony-stimulating factor (GM-CSF) activity in serum. A synthetic analogue of its N-terminal lipopeptide with ability to activate macrophages (MALP-2) was also able to induce GM-CSF in serum. Interestingly, GM-CSF induction by a low dose of MALP-2 was not associated with significant leukopenia. These data revealed that the in vitro moduline properties of mycoplasmal lipoproteins and lipopeptides correlate with interesting in vivo immunomodulatory effects.
Romero
Francisco
F
Department of Microbiology, Faculty of Pharmacy, University of Granada, 18071 Granada, Spain.
Moreno
Encarnación
E
Ruiz-Bravo
Alfonso
A
Jiménez-Valera
María
M
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000097:blood*; D055666:Lipopeptides; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000276:immunology; D008074:Lipoproteins / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D008213:Lymphocyte Activation; D008262:Macrophage Activation; D008565:Membrane Proteins / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D016858:Mycoplasma fermentans / Q000737:chemistry / Q000276:immunology*; D009842:Oligopeptides / Q000008:administration & dosage / Q000138:chemical synthesis / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013163:Splenomegaly / Q000139:chemically induced
D055666:Lipopeptides; D008070:Lipopolysaccharides; D008074:Lipoproteins; D008565:Membrane Proteins; D009842:Oligopeptides; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; C106335:macrophage stimulatory lipopeptide 2