Preliminary results of random amplification of polymorphic DNA among Triatominae of the phyllosoma complex (Hemiptera, Reduviidae).
In Mexico, Triatoma longipennis (Usinger), Triatoma picturata (Usinger), and Triatoma pallidipennis (Stal), primary Chagas disease vector species of the phyllosoma complex, were analyzed by randomly amplified polymorphic DNA (RAPD). Sixteen decametric primers resolved individual profiles not identical, but partially discriminative between species. Analysis based on pairwise presence/absence comparisons between the three species was performed using three primers and two outgroup species Triatoma infestans (Klug) and Triatoma barberi (Usinger). Fifty-three bands in total were scored, although only two bands were constant among the three phyllosoma complex species. Two other bands were constant only for T. longipennis and T. picturata together, and not present in T. pallidipennis. Neighbor Joining tree and the multiple correspondence analysis discriminated T. pallidipennis clearly from the other two species, although there was overlap between T. longipennis and T. picturata. The results indicate a close relationship between the studied species and support the hypothesis of their recent evolution. The suitability of RAPD to discern populations within the species is discussed.
Brenière
Simone F
SF
Institut de Recherche pour le Développement, UR 008, Pathogénie des Trypanosomatidés, 911 Av. Agropolis, BP 64501, 34394 Montpellier, France. breniere@mpl.ird.fr
Taveira
Bruno
B
Bosseno
Marie-France
MF
Ordoñez
Rosalinda
R
Lozano-Kasten
Felipe
F
Magallón-Gastélum
Ezequiel
E
Ouaissi
Ali
A
Ramsey
Janine
J
D000818:Animals; D005260:Female; D005819:Genetic Markers; D007303:Insect Vectors / Q000145:classification / Q000235:genetics*; D008297:Male; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique; D014225:Triatominae / Q000145:classification / Q000235:genetics*
D005819:Genetic Markers