Contribution of the antibodies response induced by a low virulent Candida albicans strain in protection against systemic candidiasis.
A low virulent Candida albicans mutant, CNC13, deleted in the Mitogen Activated Protein (MAP) kynase HOG1 was used to immunize BALB/c mice. Hog1p is essential for the oxidative stress and hyperosmolarity responses. Several doses and immunization procedures were employed. The protection capacity of the different sera generated was analyzed in a murine model of systemic candidiasis. Using a proteomic approach (two-dimensional gel electrophoresis followed by Western blotting), we were able to distinguish two categories of serum: protective and nonprotective, which showed different titres of total Immunoglobulins (Igs) and IgG2a (analyzed by enzyme-linked immunosorbent assay). The levels of Igs and IgG2a in protective sera were significantly higher compared to nonprotective sera. The pattern of a "nonprotective" profile was composed of enolase (Eno1p), transketolase, heat shock protein and methionine synthase. Only antibodies against enolase are the IgG2a isotype. The pattern of a "protective" sera, on the other hand, was composed of antibodies against the following antigens: several isoforms of Eno1p, pyruvate decarboxylase, pyruvate kynase, a protein of the 40S ribosomal subunit, triosephosphate isomerase, DL-glycerol phosphatase and fructose-bisphosphate aldolase. All these antibodies are the IgG2a isotype. The proteins described in the protective sera might be useful for future vaccine development.
Fernández-Arenas
Elena
E
Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Molero
Gloria
G
Nombela
César
C
Diez-Orejas
Rosalía
R
Gil
Concha
C
D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / Q000276:immunology; D000818:Animals; D000946:Antigens, Fungal / Q000276:immunology; D002176:Candida albicans / Q000378:metabolism; D002177:Candidiasis / Q000378:metabolism* / Q000382:microbiology; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D006360:Heat-Shock Proteins / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology*; D007132:Immunoglobulin Isotypes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics; D009154:Mutation / Q000235:genetics; D010751:Phosphopyruvate Hydratase / Q000276:immunology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D014174:Transketolase / Q000276:immunology*
D000946:Antigens, Fungal; D006360:Heat-Shock Proteins; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D029701:Saccharomyces cerevisiae Proteins; D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase; D014174:Transketolase; C088589:HOG1 protein, S cerevisiae; D020928:Mitogen-Activated Protein Kinases; D010751:Phosphopyruvate Hydratase