[Inhibitory effects of antisense TGF beta1 on in vitro and in vivo proliferation of human bladder cancer cells].
OBJECTIVE
To investigate the inhibitory effects of antisense TGF beta1 on proliferation of human bladder transitional cell carcinoma in vitro and in vivo.


METHODS
Human bladder carcinoma cell line EJ was transfected with pRevT beta-AS, a replication defective retroviral vector carried antisense TGF beta1 fragment. The growth of the transfected cells was observed in vitro and in vivo. TGF beta1 mRNA expression and protein expression were detected by RT-PCR and ELISA. The proliferative activity was evaluated by immunohistochemistry method. The ultrastructure of cells was observed by image analysis system and electron microscopy. Cell cycle was determined by flow cytometry.


RESULTS
The expression of TGF beta1 mRNA and protein in EJ cells was inhibited by pRevT beta-AS, G(1) to S transition was restrained in cell cycle and cell proliferation decreased in vitro. The tumorigenesis and growth of EJ cells and DNA heteroploidy were reduced by antisense TGF beta1 in vivo.


CONCLUSIONS
TGF beta1 plays a role in vitro proliferation and in vivo growth of bladder transitional cell carcinoma.
Yao
Xin
X
Department of Pelvic & Urologic Oncology, The Cancer Hospital of Tianjin Medical University, Tianjin 300060, China.
Chang
Ji-wu
JW
Li
Wen-lu
WL
Niu
Rui-fang
RF
Sun
Bao-cun
BC
Ma
Teng-xiang
TX
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D016372:RNA, Antisense / Q000627:therapeutic use*; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors* / Q000235:genetics; D053773:Transforming Growth Factor beta1; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy* / Q000473:pathology
D016372:RNA, Antisense; C508960:TGFB1 protein, human; C508961:Tgfb1 protein, mouse; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1