Inhibitory effect of oxymatrine on serum hepatitis B virus DNA in HBV transgenic mice.
OBJECTIVE
To study the inhibitory effect of oxymatrine on serum hepatitis B virus (HBV) DNA in HBV transgenic mice.


METHODS
HBV transgenic mice model was established by microinjection, and identified by HBV DNA integration and replication. Transgenic mice with replicating HBV were divided into 3 groups, and injected with normal saline (group A, n=9), 50 mg/kg (group B, n=8) and 100 mg/kg (group C, n=9) oxymatrine intraperitoneally once a day for 30 d, respectively. Quantitation of serum HBV DNA in HBV transgenic mice was performed by competitive polymerase chain reaction (PCR) in combination with DNA hybridization quantitative detection technique before and after treatment.


RESULTS
Compared with pre-treatment, the serum HBV DNA in group A (F=1.04, P=0.9612) and group B (F=1.13, P=0.8739) had no changes after treatment. However, in group C serum HBV DNA was significantly decreased (F=13.97, P=0.0012). The serum HBV DNA after treatment was lower in group C than in groups B and A (F=8.65, P=0.0068; F=12.35, P=0.0018; respectively). The serum HBV DNA after treatment was lower in group B than in group A, but there was no statistical significance (F=1.43, P=0.652).


CONCLUSIONS
Oxymatrine has inhibitory effects on serum HBV DNA in HBV transgenic mice.
Lu
Lun-Gen
LG
Shanghai Institute of Digestive Disease, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China. lulungen@online.sh.cn
Zeng
Min-De
MD
Mao
Yi-Min
YM
Fang
Jing-Yuan
JY
Song
Yu-Lin
YL
Shen
Zhao-Hui
ZH
Cao
Ai-Ping
AP
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D004279:DNA, Viral / Q000097:blood; D006515:Hepatitis B virus / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D019694:Hepatitis B, Chronic / Q000188:drug therapy* / Q000821:virology; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D011807:Quinolizines; D016662:Virus Integration / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects; D000093842:Matrines
D000470:Alkaloids; D000998:Antiviral Agents; D004279:DNA, Viral; D011807:Quinolizines; C037573:oxymatrine; D000093842:Matrines