Three-dimensional architecture and distribution of collagen components in the goat hypophysis.
The three-dimensional architecture of collagen fibrils in the connective tissue framework and the distribution of collagen types in the goat hypophysis were studied by the cell maceration method in combination with scanning electron microscopy (SEM) and immunohistochemistry. The pars distalis of the adenohypophysis consisted of many cell clusters. SEM revealed that the wall of cell clusters appeared as various-sized flat bundles of collagen fibrils woven in a basket-like configuration. In the pars tuberalis, the aggregates of collagen fibrils were denser and bundles thicker compared to the pars distalis. The density of collagen fibrils changed from the pars tuberalis to pars distalis without a distinct border. The collagen framework in the pars intermedia was mainly divided into three parts, the dorsal region with large hollows, the middle region, and the ventral sheet facing the cavum hypophysis. In the lobus nervosus of the neurohypophysis, the collagen network exhibited a sponge-like appearance at low magnification. Collagen fibrils of various sizes consisted of loose wavy bundles distributed around the cavities. Immunohistochemistry revealed types I, III, IV, V, and VI collagen throughout the hypophysis. It is concluded that to maintain structural and functional integration, the components of collagen are in different configurations throughout the regions of the goat hypophysis.
Nishimura
Shotaro
S
Department of Animal and Marine Bioresource Sciences, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka-shi, Japan. shotaro@agr.kyushu-u.ac.jp
Tabata
Shoji
S
Nakamura
Yoshi-Nori
YN
Okano
Kaoru
K
Iwamoto
Hisao
H
D000818:Animals; D003094:Collagen / Q000737:chemistry / Q000145:classification / Q000648:ultrastructure*; D005260:Female; D006041:Goats / Q000033:anatomy & histology*; D021621:Imaging, Three-Dimensional; D008297:Male; D010903:Pituitary Gland, Anterior / Q000033:anatomy & histology* / Q000737:chemistry / Q000648:ultrastructure; D010904:Pituitary Gland, Posterior / Q000033:anatomy & histology* / Q000737:chemistry / Q000648:ultrastructure; D014018:Tissue Distribution
D003094:Collagen