Non-replicating mucosal and systemic vaccines: quantitative and qualitative differences in the Ag-specific CD8(+) T cell population in different tissues.
Directed dissemination of Ag-specific CD8(+) T cells to infected organs or cancerous tissues is a prerequisite for optimal immunotherapy. Ag-specific CD8(+) T cells were quantitated in systemic and mucosal tissues after nasal, rectal, or cutaneous immunization with CTL epitope peptide and the adjuvant cholera toxin (CT). Mucosal and cutaneous immunization induced Ag-specific CD8(+) lymphocytes that were detectable in both mucosal and systemic compartments, suggesting a less strict distribution pattern than that known for B cells. However, optimal localization, activation and phenotype of these cells correlated with the route of immunization. In accordance with this observation, protection against a mucosal challenge with a virus expressing the CTL epitope was superior in mucosally-immunized animals.
Qimron
Udi
U
Department of Microbiology and Immunology, Faculty of Health Sciences and the Cancer Research Center, Ben Gurion University of the Negev, Beer Sheva 84105, Israel.
Paul
Lada
L
Bar-Haim
Erez
E
Bloushtain
Noga
N
Eisenbach
Lea
L
Staats
Herman F
HF
Porgador
Angel
A
D000276:Adjuvants, Immunologic; D000818:Animals; D000918:Antibody Specificity / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002772:Cholera Toxin / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D018928:Immunity, Mucosal / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D009092:Mucous Membrane / Q000276:immunology; D010641:Phenotype; D014018:Tissue Distribution; D014612:Vaccines / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000939:Epitopes; D014612:Vaccines; D002772:Cholera Toxin