Expression analysis of the entire MMP and TIMP gene families during mouse tissue development.
Matrix metalloproteinases (MMPs) and adamalysins (ADAMs) cleave many extracellular proteins, including matrix, growth factors, and receptors. We profiled the RNA levels of every MMP, several ADAMs, and inhibitors of metalloproteinases (TIMPs and RECK) in numerous mouse tissues during development and in the uterus during pregnancy. Observations include: most secreted MMPs are expressed at low to undetectable levels in tissues, whereas membrane-bound MMPs, ADAMs and inhibitors are abundant; almost every proteinase and inhibitor is present in the uterus or placenta at some time during gestation; the mouse collagenases mColA and mColB are found exclusively in the uterus and testis; and each tissue has its unique signature of proteinase and inhibitor expression.
Nuttall
Robert K
RK
School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK.
Sampieri
Clara L
CL
Pennington
Caroline J
CJ
Gill
Sean E
SE
Schultz
Gilbert A
GA
Edwards
Dylan R
DR
D000818:Animals; D000831:Animals, Newborn; D005260:Female; D058851:GPI-Linked Proteins; D015870:Gene Expression; D020869:Gene Expression Profiling; D008297:Male; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice / Q000196:embryology* / Q000254:growth & development*; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D014018:Tissue Distribution; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism
D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; C492025:Reck protein, mouse; D019714:Tissue Inhibitor of Metalloproteinases; D020782:Matrix Metalloproteinases