Killing of Bacillus subtilis spores by a modified Fenton reagent containing CuCl2 and ascorbic acid.
Bacillus subtilis spores were killed by CuCl(2)-ascorbic acid, chloride ions were essential for killing of spores, and spores with defective coats were killed more rapidly. CuCl(2)-ascorbic acid did not damage spore DNA, and spores killed by this reagent initiated germination. However, spores killed by CuCl(2)-ascorbic acid may have damage to their inner membrane.
Shapiro
Michael P
MP
Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA.
Setlow
Barbara
B
Setlow
Peter
P
D001205:Ascorbic Acid / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects*; D003300:Copper / Q000032:analysis; D004249:DNA Damage; D004269:DNA, Bacterial / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000494:pharmacology*; D007501:Iron / Q000737:chemistry / Q000494:pharmacology*; D013171:Spores, Bacterial / Q000187:drug effects
D004269:DNA, Bacterial; C045076:Fenton's reagent; D003300:Copper; D006861:Hydrogen Peroxide; D007501:Iron; D001205:Ascorbic Acid; C029892:cupric chloride