Unequal access of chromosomal regions to each other in Salmonella: probing chromosome structure with phage lambda integrase-mediated long-range rearrangements.
We have investigated the fluidity of the Salmonella chromosome architecture using the phage lambda site-specific recombination system as a probe. We determined how chromosome position affects the extent of integrase-mediated recombination between pairs of inversely oriented att sites at various loci. We also investigated the accessibility of each chromosomal att site to an extrachromosomal partner carried on a low-copy plasmid. Recombination events were assayed by semi-quantitative polymerase chain reaction of the attP product. The extent of recombination between the chromosome and the plasmid was generally higher than intrachromosomal recombination except for two loci, araA::attL and galT::attL, which gave no detectable recombination with any other locus. Based on 20 intervals, we found that chromosomal locations are not equally accessible to each other. Although multiple factors probably affect accessibility, the most important is the specific combination of the end-points used. Neither the size of the intervals nor the accessibility of individual end-points to extrachromosomal sequences is as important. These results suggest that the chromosome is not completely fluid but rather organized in some way, with barriers that limit the movement of DNA within the cell. The nature of the barriers involved in chromosomal organization remains to be determined.
Garcia-Russell
Nathalie
N
Department of Biology and Center for Microbial Sciences, San Diego State University, 5500 Campanile Dr. LS416, San Diego, CA 92182-4614, USA.
Harmon
Timothy G
TG
Le
Tien Q
TQ
Amaladas
Nelusha H
NH
Mathewson
Richard D
RD
Segall
Anca M
AM
D001287:Attachment Sites, Microbiological / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D010582:Bacteriophage lambda / Q000235:genetics*; D001435:Bacteriophages / Q000201:enzymology* / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000502:physiology* / Q000648:ultrastructure; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019426:Integrases / Q000378:metabolism; D010957:Plasmids; D011995:Recombination, Genetic; D012486:Salmonella typhimurium / Q000235:genetics*
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004279:DNA, Viral; D019426:Integrases