title: Nuclear matrix protein expressions in hepatocytes of normal and cirrhotic rat livers under normal and regenerating conditions.
abstract: We explored the feasibility of studying nuclear matrix protein (NMP) expressions of the hepatocytes in normal and cirrhotic rat livers with liver regeneration after partial hepatectomy. Sixteen Wistar healthy rats were studied with experimental liver regeneration and/or liver cirrhosis. Two-dimensional (2-D) gel electrophoresis was used to generate these NMP compositions from these rat liver samples. Several antibodies against cytokeratin, vimentin, actin, B23, HNF4alpha, and heat shock protein 70 were used for identification by Western blot. Totally, 41 strongly stained protein spots were characterized on the 2-D gels. Thirty-four protein spots were detected in all of these rat livers, of which, cytokeratin, vimentin, actin, HNF4alpha, and heat shock protein 70 were identified. B23 was detected in the regenerated livers. Three protein spots (s33, s34, and s35) were detectable only in NMP preparation extracted from the regenerating rat livers after hepatectomy. Another three protein spots (s36, s37, and s38) were detectable only in NMP preparation extracted from thioacetamide-induced cirrhotic rat livers. Under these conditions including experimental liver regeneration and/or liver cirrhosis, Over thirty higher abundance NMPs of hepatocytes were consistently expressed and considered as common and basic NMPs. Some of the NMPs are specific for liver regeneration and may play a critical role in cell proliferation and cell cycle, and some are specific for liver cirrhosis.
authors.affiliation: Department of Pathology, Cancer Center of Sun Yat-Sen University, Guangzhou, China. jpyun@gzsums.edu.cn
authors.forename: Jing-Ping
authors.initials: JP
authors.lastname: Yun
authors.forename: Choong-Tsek
authors.initials: CT
authors.lastname: Liew
authors.forename: Eng Ching
authors.initials: EC
authors.lastname: Chew
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Yin
authors.forename: Paul Bo San
authors.initials: PB
authors.lastname: Lai
authors.forename: Yam Hin
authors.initials: YH
authors.lastname: Fai
authors.forename: H K Richard
authors.initials: HK
authors.lastname: Li
authors.forename: Mei-Lin
authors.initials: ML
authors.lastname: Jin
authors.forename: Ming-Xiao
authors.initials: MX
authors.lastname: Ding
authors.forename: Ming-Tao
authors.initials: MT
authors.lastname: Li
authors.forename: Han-Liang
authors.initials: HL
authors.lastname: Lin
authors.forename: Wan Yee
authors.initials: WY
authors.lastname: Lau
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D022781:Hepatocytes / Q000473:pathology / Q000502:physiology*; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D034521:Nuclear Matrix-Associated Proteins / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D013853:Thioacetamide / Q000633:toxicity
chemical_list: D034521:Nuclear Matrix-Associated Proteins; D013853:Thioacetamide