Mfd, the bacterial transcription repair coupling factor: translocation, repair and termination.
Mfd is a widely conserved bacterial protein that couples DNA repair with transcription. Mfd recognizes RNA polymerase stalled at a non-coding template site of DNA damage, disrupts the transcription complex to release the transcript and enzyme, and recruits the DNA excision repair machinery to the site. The mechanism of RNA release has been illuminated by the discovery that Mfd causes forward translocation of RNA polymerase, using an ATP-dependent motor that is highly homologous to that of the Holliday branch migration protein RecG.
Roberts
Jeffrey
J
Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA. jwr7@cornell.edu
Park
Joo-Seop
JS
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D004260:DNA Repair / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic / Q000502:physiology*
D001426:Bacterial Proteins; D014157:Transcription Factors; C072103:transcription repair coupling factor protein, Bacteria