Lectins induce resistance to proteases and/or mechanical stimulus in all examined cells--including bone marrow mesenchymal stem cells--on various scaffolds.
Transplantation of bone marrow mesenchymal stem cells (MSC), chondrocytes, osteoblasts, or muscle cells promotes regeneration. However, these cells adhere poorly to some scaffolds--depending upon the scaffold material--and are often damaged by proteases or mechanical stimuli at site of transplantation. We found, however, that MSC, chondrocytes, and osteoblasts--along with some other cells--that were exposed to phaseolus vulgaris erythroagglutinin (PHA-E) or concanavalin A (ConA) increased their adhesion capacity on plastic tissue culture dishes and on plates of hydroxyapatite, titanium and poly-DL-lactic-co-glycolic acid (PLGA), and that these cells, moreover, built up resistance to proteases and/or mechanical stimuli. Thus, lectins may have great potential in tissue engineering and cell therapy.
Nishimura
Haruki
H
Department of Prosthetic Dentistry, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734-8553, Japan.
Nishimura
Masahiro
M
Oda
Ryo
R
Yamanaka
Katsuyuki
K
Matsubara
Takehiro
T
Ozaki
Yoshie
Y
Sekiya
Kensuke
K
Hamada
Taizo
T
Kato
Yukio
Y
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D010450:Endopeptidases / Q000378:metabolism*; D005269:Femur; D006801:Humans; D007085:Ilium; D007700:Kinetics; D037102:Lectins / Q000494:pharmacology*; D045170:Matrix Attachment Regions / Q000502:physiology*; D008648:Mesoderm / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011480:Protease Inhibitors / Q000494:pharmacology; D011817:Rabbits; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
D037102:Lectins; D011480:Protease Inhibitors; D003208:Concanavalin A; D010450:Endopeptidases