Effects of basic fibroblastic growth factor on the growth of human medulloblastoma xenografts.
The purpose of this study was to evaluate the effect of basic fibroblastic growth factor (bFGF) on the growth of human UM-MB1 medulloblastoma xenografts injected intracranially in nude mice. Under general anesthesia, a homogenous suspension of UM-MB1 cells (10(5)/10 microl) were injected in the caudoputamen nuclei of the right cerebral hemisphere using a stereotaxic apparatus. The treatment group (n = 9) received 10 microl of a bFGF solution (20 microg/ml) at 3 and 6 days following the inoculation of the cells at the injection site using the same stereotaxic coordinates. The control group (n = 9) were injected intracranially with a phosphate-buffered saline vehicle using a similar protocol. Mice were sacrificed 3 weeks following the xenograft surgery and the brains were prepared for histological observations as well as tumor volume evaluations. The mean volume of bFGF-treated tumors (mean volume +/- SD = 50.0 +/- 32.9 +/- mm3) was significantly smaller than for the non-treated xenografts (mean volume +/- SD = 199.0 +/- 42.1 mm3) (t-test, p < 0.001). Compared to non-treated tumor cells, bFGF-treated medulloblastoma cells had a greater cytoplasm volume and their nuclei contained more euchromatin suggesting that bFGF may initiate differentiation. In conclusion, our results suggest that bFGF may offer a new chemotherapeutic modality for the treatment of medulloblastoma.
Vachon
Pascal
P
Département de Biomédecine Vétérinaire, Faculté de Médecine Vétérinaire, Université de Montreal, St-Hyacinthe, QC, Canada. pascal.vachon@umontreal.ca
Girard
Christiane
C
Théorêt
Yves
Y
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage*; D006801:Humans; D007276:Injections, Intraventricular; D008527:Medulloblastoma / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000188:drug therapy*; D014183:Transplantation, Heterologous
D016222:Fibroblast Growth Factor 2