Identification of hydroxy fatty acids by liquid chromatography-atmospheric pressure chemical ionization mass spectroscopy in Euglena gracilis.
Hydroxy fatty acids from Euglena gracilis were identified by reverse-phase high performance liquid chromatography coupled to a mass spectrometer run in atmospheric pressure chemical ionization positive ion mode. These metabolites were converted to methyl esters to improve stability and chromatographic properties. A detection limit of 20 pg/microl per injection was determined for 5-HETE methyl ester based on the signal to noise ratio of the m/z 317 ion which corresponds to the loss of a hydroxyl group (M-17) and the major fragment in all HETE methyl esters studied. This is the first report for these metabolites in E. gracilis.
Santiago-Vázquez
Lory Z
LZ
Department of Ecology, Evolution and Marine Biology, University of California, Santa Barbara, CA 93106, USA.
Mydlarz
Laura D
LD
Pavlovich
James G
JG
Jacobs
Robert S
RS
D000818:Animals; D001274:Atmospheric Pressure; D002853:Chromatography, Liquid / Q000379:methods*; D005056:Euglena gracilis / Q000737:chemistry*; D005227:Fatty Acids / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005227:Fatty Acids