title: Spectrophotometric determination of glucosamine and its analogous amino sugars with o-hydroxyhydroquinonephthalein and palladium(II).
abstract: A simple and highly sensitive spectrophotometric method for the determination of glucosamine and its analogous amino sugars was established based on fading of the palladium(II)-o-hydroxyhydroquinonephthalein-hexadecyltrimethylammonium complex. In the determination of glucosamine, Beer's law is obeyed in the range of 0.02 - 0.18 microg ml(-1), with an effective molar absorptivity at 630 nm and the relative standard deviation being 8.4 x 10(5) dm3 mol(-1) cm(-1) and 1.08% (n = 10). This method is about 70-times more sensitive than the Elson-Morgan method. The method was successfully applied to the assay of glucosamine in actual samples.
authors.affiliation: Osaka University of Pharmaceutical Sciences, Nasahara, Takatsuki, Osaka 569-1094, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Mayumi
authors.initials: M
authors.lastname: Inoue
authors.forename: Kanako
authors.initials: K
authors.lastname: Miyachi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tominaga
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Fujita
mesh_terms: D000606:Amino Sugars / Q000032:analysis*; D016477:Artifacts; D002138:Calibration; D005944:Glucosamine / Q000032:analysis*; D006873:Hydroquinones / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry*
chemical_list: D000606:Amino Sugars; D006873:Hydroquinones; C039209:2-hydroxyhydroquinonephthalein; D010165:Palladium; D005944:Glucosamine