Expression of genes for B7-H3 and other T cell ligands by nasal epithelial cells during differentiation and activation.
Epithelial cells of the human respiratory tract express human leukocyte antigen (HLA) and the costimulatory molecules B7-1 and B7-2. Little is known, however, about the constitutive expression of genes encoding for the more recently identified members of the B7 homolog family of costimulatory molecules or about the influence of cellular differentiation and cytokines on their activity or on that of HLA or B7-1 and B7-2. Human nasal epithelial (HNE) cells were grown at the air-liquid interface (ALI) for 2 or 21 days to model in vivo conditions. Expression of genes for HLA-B and HLA-DR1 increased during mucociliary differentiation during this period and became more similar to HNE cells obtained fresh by brush biopsy from nasal turbinates. Gene transcripts for B7-H3 and B7-H2 were abundantly expressed in cells cultured at the ALI, but neither their activities nor that of B7-2 was significantly altered during differentiation. IFN-gamma and TNF-alpha upregulated mRNA encoding for both HLA molecules, but not for the B7 molecules. This study describes, for the first time, the expression of B7-H3 and B7-H2 by HNE cells and thus expands the range of potential costimulatory signals through which these cells may interact with activated mucosal T lymphocytes. In addition, the results suggest that the extent of mucociliary differentiation of cultured cells may influence this capability.
Saatian
Bahman
B
Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA.
Yu
Xiao-Ying
XY
Yu
XiaoYing
X
Lane
Andrew P
AP
Doyle
Thanh
T
Casolaro
Vincenzo
V
Spannhake
Ernst Wm
EW
D015703:Antigens, CD; D001706:Biopsy / Q000379:methods; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D015235:HLA-B Antigens / Q000235:genetics; D015798:HLA-DR1 Antigen / Q000235:genetics; D006801:Humans; D060945:Inducible T-Cell Co-Stimulator Ligand; D007371:Interferon-gamma / Q000494:pharmacology; D008024:Ligands; D008297:Male; D009297:Nasal Mucosa / Q000378:metabolism* / Q000473:pathology*; D011506:Proteins; D013601:T-Lymphocytes / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D015703:Antigens, CD; D002352:Carrier Proteins; D015235:HLA-B Antigens; D015798:HLA-DR1 Antigen; C498514:ICOSLG protein, human; D060945:Inducible T-Cell Co-Stimulator Ligand; D008024:Ligands; D011506:Proteins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma