title: Field evaluation of a novel colorimetric method--double-site enzyme-linked lactate dehydrogenase immunodetection assay--to determine drug susceptibilities of Plasmodium falciparum clinical isolates from northwestern Thailand.
abstract: A double-site enzyme-linked lactate dehydrogenase enzyme immunodetection assay was tested against field isolates of Plasmodium falciparum for assessing in vitro drug susceptibilities to a wide range of antimalarial drugs. Its sensitivity allowed the use of parasite densities as low as 200 parasites/microl of blood. Being a nonisotopic, colorimetric assay, it lies within the capabilities of a modest laboratory at the district level.
authors.affiliation: Shoklo Malaria Research Unit, Mae Sot, Tak 63110, Thailand. alan.brockman@menzies.edu.au
authors.forename: Alan
authors.initials: A
authors.lastname: Brockman
authors.forename: Sittaporn
authors.initials: S
authors.lastname: Singlam
authors.forename: Lucy
authors.initials: L
authors.lastname: Phiaphun
authors.forename: Sornchai
authors.initials: S
authors.lastname: Looareesuwan
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: White
authors.forename: François
authors.initials: F
authors.lastname: Nosten
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D003124:Colorimetry; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000737:chemistry*; D016778:Malaria, Falciparum / Q000469:parasitology*; D017711:Nonlinear Dynamics; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000201:enzymology*; D013785:Thailand
chemical_list: D000962:Antimalarials; D007770:L-Lactate Dehydrogenase