Expression and roles of Cl- channel ClC-5 in cell cycles of myeloid cells.
ClC-5 is a chloride channel known to be expressed in the kidney. We previously reported that ClC-5 mRNA was also strongly expressed in immature human myeloid cell line (HL-60), but weakly expressed in mature neutrophils. To clarify the underlying mechanisms, we examined the relationship between ClC-5 expression and cell cycle. Dimethyl sulfoxide treatment of HL-60 that causes differentiation with G0/G1 cell cycle arrest decreased the expression of ClC-5 mRNA. Cell sorting and synchronization experiments revealed that ClC-5 mRNA expression was high in S and G2/M phases and low in G0/G1 phase. ClC-5 antisense oligonucleotide suppressed proliferation of HL-60 cells with a decrease in ClC-5 protein expression, probably due to G2 arrest. These results suggest that cell cycle-dependent expression of ClC-5 has a role in cell cycle progression in myeloid cells.
Jiang
Baohong
B
Department of Pharmacology, Kansai Medical University, Osaka 570-8506, Japan.
Hattori
Naoki
N
Liu
Bing
B
Nakayama
Yasuhisa
Y
Kitagawa
Kaori
K
Sumita
Kiminobu
K
Inagaki
Chiyoko
C
D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation; D018118:Chloride Channels / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000032:analysis; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D007399:Interphase; D022423:Myeloid Cells / Q000166:cytology* / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis
C097503:CLC-5 chloride channel; D018118:Chloride Channels; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D004247:DNA; D004121:Dimethyl Sulfoxide