Immobilized 2-(4-hydrazinocarbonylphenyl)-4,5-diphenylimidazole as solid phase luminophore in peroxyoxalate chemiluminescence.
A new luminophore for application in peroxyoxalate chemiluminescence is presented. An analogue of the well-known chemiluminescence compound lophine, i.e. 2-(4-hydrazinocarbonylphenyl)-4,5-diphenylimidazole (HCPI), has been covalently immobilized to controlled pore glass and a porous methacrylate resin. By using this reagent in a solid phase detection reactor, sensitive determinations of hydrogen peroxide have been demonstrated. In homogeneous solution HCPI emits poorly as a result of 1,1'-oxalyldiimidazole excitation, but when immobilized its efficiency is almost comparable to highly efficient luminophores such as 3-aminofluoranthene. Linearity extends in the single stream flow system over several orders of magnitude with both materials. The limit of detection was 1 nmol/l (10 fmole injected), when using the porous methacrylate support.
Pontén
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E
Department of Analytical Chemistry, University of Umeå, S-901 87, Umeå, Sweden.
Appelblad
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P
Stigbrand
M
M
Irgum
K
K
Nakashima
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K