[Effects of interleukin on orbital fibroblasts of patients with Graves' ophthalmopathy].
OBJECTIVE
To observe the effects of interleukin including interleukin (IL)-1, interleukin (IL)-2, interleukin (IL)-6 on proliferation of orbital fibroblasts (OFs) and on secretion of hyaluronic acid (HA) in vitro.


METHODS
OFs from 5 patients with Graves' ophthalmopathy (GO) and from 4 normal control subjects were cultured in vitro. IL-1, IL-2, IL-6 with different concentrations were incubated with cultured OFs. The proliferation of OFs and the production of HA were measured by MTT and radioimmunological technique.


RESULTS
IL-1 (5, 50, 500 U/ml), IL-2 (500 U/ml) and IL-6 (500 U/ml) could significantly stimulate the proliferation of OFs and the secretion of HA. At the concentration of 500 U/ml, IL-1 had stimulating effects on patients' OFs more than on normal subjects' OFs.


CONCLUSIONS
IL-1, IL-2, IL-6 can stimulate proliferation of OFs and secretion of HA. The disturbance of interleukin may be one aspect of the pathogenesis of GO.
Zhang
Xue-jin
XJ
Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, China.
Luo
Qing-li
QL
Zhang
Mei-xia
MX
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000473:pathology*; D006111:Graves Disease / Q000473:pathology*; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism; D007375:Interleukin-1 / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D007378:Interleukins / Q000494:pharmacology*; D009915:Orbit / Q000473:pathology*
D007375:Interleukin-1; D007376:Interleukin-2; D015850:Interleukin-6; D007378:Interleukins; D006820:Hyaluronic Acid