Fatty acids of serine, ethanolamine, and choline plasmalogens in some marine bivalves.
The FA composition of glycerophospholipid (GPL) classes and subclasses was investigated in whole animals of three marine bivalve mollusks: the Japanese oyster Crassostrea gigas, the blue mussel Mytilus edulis, and the Manila clam Ruditapes philippinarum. Individual organs (gills, mantle, foot, siphon, and muscle) of the Manila clam also were examined. The PS plasmalogen (PSplsm), PE plasmalogen (PEplsm), and PC plasmalogen (PCplsm) subclasses were isolated by HPLC, and their individual FA compositions were examined using GC. Plasmalogen forms of PS and PE, when compared to their respective diacyl forms, were found to be specifically enriched with non-methylene-interrupted (NMI) FA (7,15-22:2, 7,13-22:2, and their precursors) and 20:1 n-11 FA. Such a clear specific association was not found for PCplsm. Interestingly, this trend was most apparent in PSplsm, and the above FA were found to be, respectively, the predominant PUFA and monounsaturated FA in the PSplsm isolated from the three species. This specificity was maintained in all the analyzed organs of the Manila clam but varied in proportions: The highest level of plasmalogens, NMI FA, and 20:1 n-11 was measured in gills and the lowest was in muscle. These results represent the first comprehensive report on a FA composition of the PSplsm subclass isolated from mollusks. The fact that NMI FA and 20:1 n-11, which are thought to be biosynthesized FA, were mainly associated with aminophospholipid plasmalogens (PE and PS) is likely to have a functional significance in bivalve membranes.
Kraffe
Edouard
E
Unité mixte Centre Nationale de la Recherche Scientifique CNRS) 6321, Université de Bretagne Occidentale CS93837, 29238 Brest Cedex 3, France.
Soudant
Philippe
P
Marty
Yanic
Y
D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis*; D010125:Ostreidae / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry / Q000145:classification; D010714:Phosphatidylethanolamines / Q000737:chemistry / Q000145:classification; D010718:Phosphatidylserines / Q000737:chemistry / Q000145:classification; D010955:Plasmalogens / Q000737:chemistry* / Q000145:classification; D012758:Shellfish / Q000032:analysis*
D005227:Fatty Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D010955:Plasmalogens