[Study of biosensor technology on the detection of endotoxin-neutralizing materials].
OBJECTIVE
To explore the application of biosensor technology in the determination of endotoxin-neutralizing materials.


METHODS
After mixing polymyxin B (PMB) with endotoxin in certain concentration, the neutralizing ratio of PMB to endotoxin was assessed by biosensor technique and limulus amebocyte lysate test respectively. The results from the two methods were compared.


RESULTS
The neutralizing ratio of PMB to endotoxin as assessed by biosensor technology was 0.35 microg to 1 ng, while that by dynamic turbidimetric and chromogenic limulus amebocyte lysate (LAL) technique was 0.5 mg to 1 ng and 1 mg to 1 ng, respectively. The results obtained by biotechnology were similar to that by biosensor technique.


CONCLUSIONS
Biosensor technology was an accurate, convenient and rapid method for the determination of potency of endotoxin-neutralizing materials.
Lu
Gen-fa
GF
Institute of Burn Research, Southwestern Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, The Third Military Medical University, Chongqing 400038, P.R. China.
Gong
Xiao-yun
XY
Wei
Guo
G
Wang
Ning
N
Xiao
Guang-xia
GX
Zheng
Jiang
J
D001426:Bacterial Proteins / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D004731:Endotoxins / Q000032:analysis*; D008050:Lipid A / Q000032:analysis*; D011112:Polymyxin B / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D001426:Bacterial Proteins; D004731:Endotoxins; D008050:Lipid A; C027315:lipid A-associated protein, Bacteria; D011112:Polymyxin B