[Detection and quantitative determination of modified and/or denatured human serum albumin].
A simple method of quantitative evaluation of modified human serum albumin (SA) was suggested for the use in clinical practice. It is based on the natural ability of modified SA to form complexes with endogenous urea. An SA content, which lost its secondary and tertiary structures, is indirectly predetermined by the quantity of urea bound with protein that is biochemically determined after hydrolysis of the studied sample of partially purified SA. The clinical value of the parameter that is being determined is under discussion.
Kozhevnikov
A D
AD
Leshenkova
E V
EV
Sazonets
G I
GI
D006801:Humans; D011485:Protein Binding; D011487:Protein Conformation; D011489:Protein Denaturation; D012709:Serum Albumin / Q000032:analysis* / Q000378:metabolism; D014508:Urea / Q000378:metabolism
D012709:Serum Albumin; D014508:Urea