The novel organization and complete sequence of the ribosomal RNA gene of Nosema bombycis.
We present here for the first time the complete DNA sequence data (4301bp) of the ribosomal RNA (rRNA) gene of the microsporidian type species, Nosema bombycis. Sequences for the large subunit gene (LSUrRNA: 2497bp, GenBank Accession No. ), the internal transcribed spacer (ITS: 179bp, GenBank Accession No. ), the small subunit gene (SSUrRNA: 1232bp), intergenic spacer (IGS: 279bp), and 5S region (114bp) are also given, and the secondary structure of the large subunit is discussed. The organization of the N. bombycis rRNA gene is LSUrRNA-ITS-SSUrRNA-IGS-5S. This novel arrangement, in which the LSU is 5' of the SSU, is the reverse of the organizational sequence (i.e., SSU-ITS-LSU) found in all previously reported microsporidian rRNAs, including Nosema apis. This unique character in the type species may have taxonomic implications for the members of the genus Nosema.
Huang
Wei-Fone
WF
Department of Entomology, National Taiwan University, Taipei 106, Taiwan, ROC.
Tsai
Shu-Jen
SJ
Lo
Chu-Fang
CF
Soichi
Yamane
Y
Wang
Chung-Hsiung
CH
D000818:Animals; D001483:Base Sequence; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D017125:Genes, Protozoan; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D016818:Nosema / Q000235:genetics*; D009690:Nucleic Acid Conformation; D017422:Sequence Analysis, DNA
D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer