Fluorescence correlation spectroscopy: a new tool for quantification of molecular interactions.
Fluorescence correlation spectroscopy (FCS) provides a powerful method to measure molecular dynamics and interactions in a wide variety of experimental systems and environments. In this article we focus on the use of FCS methods to quantify molecular interactions, including the use of diffusion analysis and molecular counting. Both autocorrelation and cross-correlation FCS measurements are discussed.
Berland
Keith M
KM
Physics Department, Emory University, Atlanta, GA.
D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods; D009841:Oligonucleotides / Q000032:analysis / Q000235:genetics; D011485:Protein Binding; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*
D009841:Oligonucleotides