[The influence of fluoride on the development of the osteoblast phenotype in rat calvarial osteoblasts: an in vitro study]
OBJECTIVE:The effects of low doses fluoride on the phenotypic development of rat fetal calvarial osteoblast were studied because the cellular mechanism of fluoride promotes the increasing of bone volume clinically is unclear. METHODS:The influence of fluoride on the rat first passage osteoblast were valuated by histochemistry, enzyme-histochemistry and electron microanalysis in vitro.RESULTS:The study had revealed that not only the bone tubular and the degree of mineralization, but also the activity expression of alkaline phosphatase of osteoblasts in fluoride group were better than the control group. CONCLUSION:The results suggested that low doses fluoride might not only increase the proliferation and differentiation of osteoblast precursors as well as the production and activity of alkaline phosphatase; but also favored the mineralized formation of osteoblast nodules.
Zhang
W G
WG
School of Stomatology, Shanghai Second Medical University. Shanghai 200011,China.
Wang
L Z
LZ
Liu
Z
Z