title: Exploitation of host cell cytoskeleton and signalling during Listeria monocytogenes entry into mammalian cells.
abstract: Deciphering how Listeria monocytogenes exploits the host cell machinery to invade mammalian cells during infection is a key issue for the understanding how this food-borne pathogen causes a pleiotropic disease ranging from gastro-enteritis to meningitis and abortions. Using multidisciplinary approaches, essentially combining bacterial genetics and cell biology, we have identified two bacterial proteins critical for entry into target cells, InlA and InlB. Their cellular ligands have been also identified: InlA interacts with the adhesion molecule E-cadherin, while InlB interacts with the receptor for the globular head of the complement factor C1q (gC1q-R), with the hepatocyte growth factor receptor (c-Met) and with glycosaminoglycans (including heparan sulphate). The dynamic interaction between these cellular receptors and the actin cytoskeleton is currently under investigation. Several intracellular molecules have been recognized as key effectors for Listeria entry into target cells, including catenins (implicated in the connection of E-cadherin to actin) and the actin depolymerising factor/cofilin (involved in the rearrangement of the cytoskeleton in the InlB-dependent internalisation pathway). At the organism level, species specificity has been discovered concerning the interaction between InlA and E-cadherin, leading to the generation of transgenic mice expressing the human E-cadherin, in which the critical role of InlA in the crossing of the intestinal barrier has been clearly determined. Listeria appears as an instrumental model for addressing critical questions concerning both the complex process of bacterial pathogenesis and also fundamental molecular processes, such as phagocytosis.
authors.affiliation: Unité des interactions bactéries-cellules, département Biologie cellulaire et Infection, Institut Pasteur, 28, rue du Docteur-Roux, 75724 Paris, France.
authors.forename: Javier
authors.initials: J
authors.lastname: Pizarro-Cerdá
authors.forename: Sandra
authors.initials: S
authors.lastname: Sousa
authors.forename: Pascale
authors.initials: P
authors.lastname: Cossart
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001426:Bacterial Proteins / Q000502:physiology*; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins; D003599:Cytoskeleton / Q000502:physiology*; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D018960:Hyaluronan Receptors; D008089:Listeria monocytogenes / Q000502:physiology*; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D024101:Mitochondrial Proteins; D008954:Models, Biological; D019859:Proto-Oncogene Proteins c-met / Q000378:metabolism; D011951:Receptors, Complement / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D000199:Actins; D001426:Bacterial Proteins; C087299:C1QBP protein, human; C490193:C1qbp protein, mouse; D015820:Cadherins; D002352:Carrier Proteins; D006025:Glycosaminoglycans; D018960:Hyaluronan Receptors; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D011951:Receptors, Complement; C032402:complement 1q receptor; C095768:inlB protein, Listeria monocytogenes; C069761:internalin protein, Bacteria; D019859:Proto-Oncogene Proteins c-met