title: NuMA expression and function in mouse oocytes and early embryos.
abstract: Nuclear mitotic apparatus protein (NuMA), originally described as a nuclear protein, is an essential component in the formation and maintenance of mitotic spindle poles. In this study, we analyze the expression pattern and function of NuMA in mouse oocytes and early embryos. In germinal vesicle-stage oocytes, NuMA was detected both at the centrosome and in the nucleus. However, after nuclear maturation and extrusion of the first polar body, NuMA was concentrated at the broad meiotic spindle poles and at cytasters (centers of cytoplasmic microtubule asters) of mature metaphase II oocytes. Cold-induced depolymerization of microtubules appeared to disassociate NuMA foci from the cytoplasmic cytasters. During fertilization, NuMA was relocated into the re-formed male and female pronuclei. Microinjection of anti-NuMA antibody into 1 of 2 cells of 2-cell-stage embryos inhibited normal cell division. These results suggest that NuMA might play an important role in cell division during early embryonic mitosis.
authors.affiliation: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, ROC.
authors.forename: Chieh-Ju C
authors.initials: CJ
authors.lastname: Tang
authors.forename: Hui-Mei
authors.initials: HM
authors.lastname: Hu
authors.forename: Tang K
authors.initials: TK
authors.lastname: Tang
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins; D004622:Embryo, Mammalian / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D051379:Mice; D008813:Mice, Inbred ICR; D009687:Nuclear Proteins / Q000378:metabolism / Q000502:physiology*; D009865:Oocytes / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; C489080:Numa1 protein, mouse