The interaction of Schwann cells with chitosan membranes and fibers in vitro.
The bridging of nerve gaps is still one of the major problems in peripheral nerve regeneration. A promising alternative for the repair of peripheral nerve injuries is the bioartificial nerve graft, comprised of a biomaterial pre-seeded with Schwann cells (SCs), which is an effective substrate for enhancing nerve regeneration. Interaction between cultured SCs and biomaterials is of importance. For the purposes of this study, culture systems of normal SCs were used. The biocompatibility of chitosan, including chitosan membranes and chitosan fibers, was evaluated in vitro. The growth of SCs was observed by light and scanning electron microscopy at regular intervals. SCs were identified by immunocytochemical staining and the viability of SCs was measured by MTT assay. The experimental results indicated that SCs could grow onto chitosan materials with two different shapes: spherical and long olivary. They contacted with the extensions. The long olivary cells inclined to encircle chitosan fibers up. It was also found that the cells on the chitosan fibers migrated faster than those on the chitosan membranes. There was a good biological compatibility between chitosan and SCs. Compared with the chitosan membranes, SCs migrated more easily onto the stereoframe of chitosan fibers. These studies contribute information necessary to enhancing our understanding of biocompatibility of chitosan.
Yuan
Ying
Y
The Jiangsu Key Laboratory of Neuroregeneration, Nantong Medical College, Nantong, Jiangsu 226001, PR China.
Zhang
Peiyun
P
Yang
Yuming
Y
Wang
Xiaodong
X
Gu
Xiaosong
X
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division; D002465:Cell Movement / Q000502:physiology*; D048429:Cell Size; D002470:Cell Survival; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D008422:Materials Testing; D009416:Nerve Regeneration / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000473:pathology*; D012584:Sciatic Nerve / Q000293:injuries / Q000473:pathology* / Q000601:surgery*; D023822:Tissue Engineering / Q000379:methods; D019737:Transplants
D001672:Biocompatible Materials; D048271:Chitosan