Specific identification of Pasteurella multocida serogroup-A isolates by PCR assay.
A polymerase chain reaction (PCR) assay targeting the hyaC-hyaD gene was developed and used to identify strains of Pasteurella multocida belonging to serogroup-A. A set of serogroup-specific-PCR primers amplified a 564 bp product from genomic DNA prepared from bacterial cells or directly from bacterial colonies. This method detected as low as 10 ng of bacterial DNA and had a specificity of 100% for P. multocida serogroup-A. A nested PCR method yielded a single 374 bp product. All fifty isolates were also shown to be identical by restriction fragment length polymorphism (RFLP) analysis of the PCR products after digestion with BglII.
Gautam
Rajeev
R
Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, U.P. 243122, India.
Kumar
A A
AA
Singh
V P
VP
Singh
Vijendra P
VP
Dutta
T K
TK
Shivachandra
S B
SB
D000818:Animals; D017931:DNA Primers; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016979:Pasteurella multocida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods / Q000662:veterinary
D017931:DNA Primers; D004269:DNA, Bacterial