[Genotyping of EsBLs-producing Escherichia coli by pulsed-field gel electrophoresis in nosocomial infection].
OBJECTIVE
Using a genotying method which is accurate and rapid to study the molecular epidemiology of EsBLs-producing Escherichia coli in nosocomial infection.


METHODS
After an analysis of clinical information to ascertain nosocomial infection, EsBLs were detected by phenotype confirmation test and pulsed-field gel electrophoresis (PFGE).


RESULTS
Three EsBLs-producing strains in ICU were verified as epidemiological-related because their dice coefficient index was higher than 95%. The others were verified as non-epidemiological-related since their different lanes were more than 6.


CONCLUSIONS
PFGE is a perfect molecular typing method to study epidemiolgy and analyze homology.
Kang
Mei
M
Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Chen
Chao-yang
CY
Norman
Hui
H
Chen
Wen-zhao
WZ
Guo
Xiao-jing
XJ
Zheng
Dong-bin
DB
D002681:China / Q000453:epidemiology; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology / Q000235:genetics; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D010641:Phenotype; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000378:metabolism*
D001618:beta-Lactamases