Biocompatibility of sorbitol-containing polyesters. Part I: Synthesis, surface analysis and cell response in vitro.
A series of sorbitol-containing polyesters were synthesized via a one-pot lipase-catalyzed condensation polymerization. Thin films were prepared by spin coating on silicon wafers and surfaces were analyzed by tapping mode atomic force microscopy and contact angle measurements. Surface morphologies and surface energies across the series of polyester films, including a poly(epsilon-caprolactone) (PCL) control were nearly indistinguishable. Biocompatibility of the sorbitol-containing polyester series was evaluated against a PCL control by measuring cell spreading and proliferation of a mouse fibroblast 3T3 cell line in vitro. Results confirmed that the sorbitol-containing polyester surfaces elicited cell behavior similar to the PCL control. These results establish the sorbitol-containing polyester series as a promising material for tissue engineering research and development.
Mei
Ying
Y
Department of Chemistry and Chemical Engineering, NSF-I/UCRC Center for Biocatalysis and Bioprocessing of Macromolecules, Polytechnic University, Six Metrotech Center, Brooklyn, NY 11201, USA.
Kumar
Ajay
A
Gao
Wei
W
Gross
Richard
R
Kennedy
Scott B
SB
Washburn
Newell R
NR
Amis
Eric J
EJ
Elliott
John T
JT
D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology*; D002455:Cell Division / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D048429:Cell Size; D002470:Cell Survival; D008422:Materials Testing; D051379:Mice; D008968:Molecular Conformation; D011091:Polyesters / Q000737:chemistry*; D013012:Sorbitol / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D011091:Polyesters; D013012:Sorbitol