Development of novel PCR markers linked to the BYDV resistance gene Bdv2 useful in wheat for marker-assisted selection.
The distal segment of the long arm of the Thinopyrum intermedium chromosome 7Ai1 carries the barley yellow dwarf virus (BYDV) resistance gene Bdv2. This segment was transferred to the distal region of the long arm of wheat chromosome 7D in the Yw series of translocation lines by using the ph1b mutant to induce homoeologous pairing. To transfer Bdv2 to commercial varieties, we developed two resistance gene-analog polymorphism (RGAP) markers, Tgp-1(350) and Tgp-2(210), and one randomly amplified polymorphic DNA (RAPD) marker, OPD04(1300). The diagnostic fragments of the RGAP marker Tgp-1(350) and the RAPD marker OPD04(1300) were cloned, sequenced and converted into sequence-characterized amplified region (SCAR) markers, named SC-gp1 and SC-D04, respectively. SC-gp1 and SC-D04 were validated based on available translocation lines and segregating F(2) individuals. Our results indicated that the SCAR markers co-segregated with the BYDV resistance associated with Bdv2. Therefore, they can be used as a low-cost, high-throughput alternative to conventional phenotypic screening in wheat-breeding programs exploiting Bdv2. The marker-assisted selection for BYDV resistance was successfully performed in a wheat-breeding program.
Zhang
Zengyan
Z
Key Laboratory of Crop Genetics and Breeding of Ministry of Agriculture, Institute of Crop Breeding and Cultivation, Chinese Academy of Agriculture Sciences, Beijing 100081, China.
Xu
Jingsheng
J
Xu
Qinjuan
Q
Larkin
Philip
P
Xin
Zhiyong
Z
D001947:Breeding / Q000379:methods; D032461:Chromosomes, Plant / Q000235:genetics*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D005819:Genetic Markers / Q000235:genetics*; D007113:Immunity, Innate / Q000235:genetics*; D017793:Luteovirus; D010641:Phenotype; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics* / Q000821:virology
D017931:DNA Primers; D005819:Genetic Markers