Design of a compact disk-like microfluidic platform for enzyme-linked immunosorbent assay.
This paper presents an integrated microfluidic device on a compact disk (CD) that performs an enzyme-linked immunosorbent assay (ELISA) for rat IgG from a hybridoma cell culture. Centrifugal and capillary forces were used to control the flow sequence of different solutions involved in the ELISA process. The microfluidic device was fabricated on a plastic CD. Each step of the ELISA process was carried out automatically by controlling the rotation speed of the CD. The work on analysis of rat IgG from hybridoma culture showed that the microchip-based ELISA has the same detection range as the conventional method on the 96-well microtiter plate but has advantages such as less reagent consumption and shorter assay time over the conventional method.
Lai
Siyi
S
Department of Chemical Engineering, Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, USA.
Wang
Shengnian
S
Luo
Jun
J
Lee
L James
LJ
Yang
Shang-Tian
ST
Madou
Marc J
MJ
D000818:Animals; D001331:Automation; D002478:Cells, Cultured; D016250:Compact Disks; D003470:Culture Media / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006825:Hybridomas / Q000166:cytology; D007136:Immunoglobulins / Q000032:analysis; D044085:Microfluidics / Q000295:instrumentation*; D051381:Rats; D013997:Time Factors
D003470:Culture Media; D007136:Immunoglobulins