[The effects of nuclear factor kappa B decoy oligodeoxynucleotides on ciglitazone-induced differentiation of lung cancer cells A549].
OBJECTIVE
To investigate the role of nuclear factor kappa B (NF-kappaB) decoy oligodeoxynucleotides (ODNs) in the process of differential regulation of the lung cancer cells A549 by ciglitazone.


METHODS
NF-kappaB decoy ODNs were transfected into A549 cells with Lipofect AMINE 2000, and electrophoretic mobility shift assay (EMSA) was performed to investigate the activation of NF-kappaB, while the level of mdr1 was observed by Western blot. When ciglitazone was added, the proliferation was observed by growth curve and the differentiation of cells was observed by flow cytometry, and the expression of cyclinD1 was observed by Western blot.


RESULTS
EMSA showed that the decoy ONDS-decreased the activation of NF-kappaB in A549, and Western blot showed that the decoy ONDS decreased the level of mdr1. Ciglitazone significantly inhibited the growth and induced the differentiation of A549 cells that were transfected with NF-kappaB decoy ODNs. There were more cells arrested in G(1)/G(0) phase and the expression of cyclinD1 was markedly down-regulated as compared to the control cells.


CONCLUSIONS
NF-kappaB decoy ODNs could enhance the effects of ciglitazone on proliferative suppression and differential induction of A549 cells.
Zhang
Hui-lan
HL
Respiratory Department of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhang
Zhen-xiang
ZX
Xu
Yong-jian
YJ
D002453:Cell Cycle; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D019450:Genes, MDR; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000235:genetics; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D045162:Thiazolidinediones / Q000494:pharmacology*; D014162:Transfection
D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; D045162:Thiazolidinediones; C039671:ciglitazone