Peanut stripe potyvirus resistance in peanut (Arachis hypogaea L.) plants carrying viral coat protein gene sequences.
Peanut (Arachis hypogaea L.) lines exhibiting high levels of resistance to peanut stripe virus (PStV) were obtained following microprojectile bombardment of embryogenic callus derived from mature seeds. Fertile plants of the commercial cultivars Gajah and NC7 were regenerated following co-bombardment with the hygromycin resistance gene and one of two forms of the PStV coat protein (CP) gene, an untranslatable, full length sequence (CP2) or a translatable gene encoding a CP with an N-terminal truncation (CP4). High level resistance to PStV was observed for both transgenes when plants were challenged with the homologous virus isolate. The mechanism of resistance appears to be RNA-mediated, since plants carrying either the untranslatable CP2 or CP4 had no detectable protein expression, but were resistant or immune (no virus replication). Furthermore, highly resistant, but not susceptible CP2 T0 plants contained transgene-specific small RNAs. These plants now provide important germplasm for peanut breeding, particularly in countries where PStV is endemic and poses a major constraint to peanut production.
Higgins
Colleen M
CM
Biotechnology, Department of Primary Industries, Queensland Agency for Food and Fibre Sciences, Queensland Bioscience Precinct, 306 Carmody Road, The University of Queensland, St. Lucia, Qld 4072, Australia.
Hall
Rhonda M
RM
Mitter
Neena
N
Cruickshank
Alan
A
Dietzgen
Ralf G
RG
D010367:Arachis / Q000235:genetics* / Q000276:immunology; D036022:Capsid Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D005818:Genetic Engineering / Q000379:methods*; D030821:Plants, Genetically Modified; D017800:Potyvirus / Q000235:genetics* / Q000472:pathogenicity; D012367:RNA, Viral / Q000235:genetics; D012038:Regeneration; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D019076:Transgenes
D036022:Capsid Proteins; D012367:RNA, Viral