Quantitative optical imaging of the pharmacokinetics of fluorescent-specific antibodies to tumor markers through tissuelike turbid media.
Fluorescent optical imaging of tumors deep within tissue depends on specific binding of antibodies to the tumors' surface markers. These fluorescent antibodies propagating in the vicinity of the tumor can be attached to and (or) diffused away from it. We illustrate application of a new tool, based on the random-walk theory in turbid media, for extracting the pharmacokinetics of these fluorescent antibodies by data deconvolution, excluding the effect of upper turbid tissue layers.
Gannot
Israel
I
Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv 69978, Israel. gannot@eng.tau.ac.il
Garashi
Avital
A
Chernomordik
Victor
V
Gandjbachkhe
Amir
A
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000276:immunology* / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000493:pharmacokinetics*; D005455:Fluorescent Antibody Technique / Q000379:methods*; D005456:Fluorescent Dyes / Q000493:pharmacokinetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008962:Models, Theoretical; D009368:Neoplasm Transplantation; D055095:Optics and Photonics; D014062:Tongue Neoplasms / Q000378:metabolism
D000906:Antibodies; D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes; D016650:Fluorescein-5-isothiocyanate