Methyl tert-butyl ether biodegradation by microbial consortia obtained from soil samples of gasoline-polluted sites in Mexico.
Microbial consortia obtained from soil samples of gasoline-polluted sites were individually enriched with pentane, hexane, isooctane and toluene. Cometabolism with methyl tert-butyl ether, (MTBE), gave maximum degradation rates of 49, 12, 32 and 0 mg g(-1)protein h(-1), respectively. MTBE was fully degraded even when pentane was completely depleted with a cometabolic coefficient of 1 mgMTBE mg(-1)pentane. The analysis of 16S rDNA from isolated microorganisms in the pentane-adapted consortia showed that microorganisms could be assigned to Pseudomonas. This is the first work reporting the cometabolic mineralization of MTBE by consortium of this genus.
Morales
Marcia
M
Programa de Medio Ambiente y Seguridad, Instituto Mexicano del Petróleo, Eje Central Lazzaro Cárdenas 152, C.P 07730, Mexico DF. mibarria@imp.mx
Velázquez
Elia
E
Jan
Janet
J
Revah
Sergio
S
González
Uriel
U
Razo-Flores
Elías
E
D000473:Alkanes / Q000378:metabolism; D001673:Biodegradation, Environmental; D001709:Biotechnology / Q000379:methods*; D002245:Carbon Dioxide / Q000378:metabolism; D004275:DNA, Ribosomal / Q000378:metabolism; D005742:Gasoline; D007700:Kinetics; D008738:Methyl Ethers / Q000378:metabolism*; D008800:Mexico; D010420:Pentanes / Q000737:chemistry; D011549:Pseudomonas / Q000378:metabolism; D012988:Soil Microbiology; D012989:Soil Pollutants; D013997:Time Factors
D000473:Alkanes; D004275:DNA, Ribosomal; D005742:Gasoline; D008738:Methyl Ethers; D010420:Pentanes; D012989:Soil Pollutants; D002245:Carbon Dioxide; C043243:methyl tert-butyl ether; C033353:pentane