Explant pathology study of decellularized carotid artery vascular grafts.
The purpose of this study was to evaluate the morphologic findings in small-diameter freeze-dried decellularized carotid artery grafts implanted in goats as carotid artery interposition grafts for 6-7 months. Unimplanted decellularized carotid artery grafts did not contain intact cells; however, remnants of smooth muscle cells were present in the media. The extracellular matrix was well preserved. All decellularized grafts were patent at explant, without significant dimensional changes or aneurysm formation. Their luminal surfaces were lined by a thin neointima, consisting of myofibroblasts, collagen, and a discontinuous layer of endothelial cells. Histologic evidence of calcification within the explants was not observed; however, electron microscopy showed calcification of minute remnants of cell membranes. Inflammatory cells were not present in the graft wall. Host cell migration was greatest in the adventitia along the length of the graft. Migration of host cells into the media was more apparent close to the anastomoses, forming cellular nests rich in extracellular proteoglycans, whereas cell migration into areas subjacent to the lumen was minimal. Ingrowth of host blood vessels was not observed. These results demonstrate satisfactory structural and morphologic features of a decellularized carotid artery small-diameter graft implanted for up to 7 months.
Hilbert
Stephen L
SL
Office of Science and Technology (HFZ-150), Center for Devices and Radiological Health, Food and Drug Administration, 9200 Corporate Boulevard, Rockville, Maryland 20850, USA. sxh@cdrh.fda.gov
Boerboom
Lawrence E
LE
Livesey
Stephen A
SA
Ferrans
Victor J
VJ
D000818:Animals; D002339:Carotid Arteries / Q000033:anatomy & histology / Q000473:pathology / Q000637:transplantation* / Q000648:ultrastructure; D006041:Goats / Q000601:surgery; D006257:Head / Q000098:blood supply; D008297:Male; D008854:Microscopy, Electron; D014680:Veins / Q000033:anatomy & histology