High-performance liquid-chromatographic determination of rifampicin in plasma and tissues.
A HPLC-UV method has been developed for assaying rifampicin in plasma and liver. The assay involved a liquid-liquid extraction procedure with dichloromethane-pentane (1:1). An Ultrabase-C18 column and a simple mobile phase consisting of a water (pH 2.27)-acetonitrile (40:60, v/v) mixture were used. The flow-rate was 1 ml/min and the effluent was monitored at 333 nm. Results from the HPLC analyses showed that the assay method is linear in the ranges 0.1-1 and 1-50 microg/ml for plasma, and 0.6-40 microg/g for liver. Intra- and inter-day R.S.D. were below 15% for all the sample types. Recoveries averaged 83 and 95% for plasma and liver, respectively. The method is being successfully applied to determine rifampicin in plasma and liver samples taken during pharmacokinetic studies in rats.
Calleja
Ignacio
I
Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Navarra, C/Irunlarrea 1, 31080 Pamplona, Spain.
Blanco-Príeto
María J
MJ
Ruz
Noelia
N
Renedo
María Jesús
MJ
Dios-Viéitez
María Carmen
MC
D000818:Animals; D000904:Antibiotics, Antitubercular / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D008099:Liver / Q000737:chemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards; D015203:Reproducibility of Results; D012293:Rifampin / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D012996:Solutions; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D014018:Tissue Distribution
D000904:Antibiotics, Antitubercular; D012996:Solutions; D012997:Solvents; D012293:Rifampin