Evaluation of bacterial adhesion of Streptococcus mutans on dental restorative materials.
Bacterial adhesion to the surface of composite resins and other dental restorative materials is an important parameter in the aetiology of secondary caries formation. The aim of the present study was to investigate the adhesion of a Streptococcus mutans strain (ATCC 25175) on the surface of different restorative materials. The test materials examined included three flowable composites (Filtek Flow, Tetric Flow, and Arabesk Flow), three microhybrid composites (Clearfil APX, Solitaire 2, and Z250), two glass-ionomers (Fuji IX, Fuji IX fast), a compomer (F2000), an ormocer (Admira), and a control reference material (tissue culture grade, surface treated polystyrene). The adhesion tests were carried out in 24-well plates. Quantitative turbidimetric measurements were finally performed in order to indirectly evaluate the amount of bacteria retained on the material surface after in vitro exposure to the bacteria suspension. Under these conditions, with the exception of the Admira ormocer and the Fuji IX fast glass ionomer, which were found to be more adhesive, all the other material surfaces showed a similar susceptibility to bacterial adhesion, exhibiting values not significantly different than the reference polystyrene control. Furthermore, the release of fluoride from some of the test surfaces did not appear capable to reduce early bacterial adhesion.
Montanaro
Lucio
L
Research Laboratory on Biocompatibility of Implant Materials, Rizzoli Orthopaedic Institute, and Experimental Pathology Department, University of Bologna, Italy. lucio.montanaro@alma.unibo.it
Campoccia
Davide
D
Rizzi
Simona
S
Donati
Maria Elena
ME
Breschi
Lorenzo
L
Prati
Carlo
C
Arciola
Carla Renata
CR
D000268:Adhesiveness; D001422:Bacterial Adhesion / Q000502:physiology; D018441:Biofilms / Q000254:growth & development*; D003764:Dental Materials / Q000737:chemistry*; D003793:Dental Restoration, Permanent / Q000379:methods*; D004058:Diffusion; D005459:Fluorides / Q000737:chemistry*; D008422:Materials Testing / Q000379:methods*; D013295:Streptococcus mutans / Q000166:cytology* / Q000502:physiology*
D003764:Dental Materials; D005459:Fluorides