Conversion of the Nikon C1 confocal laser-scanning head for multiphoton excitation on an upright microscope.
Most commercial laser-scanning imaging systems used for confocal fluorescence microscopy can be readily adapted for use with two-photon fluorescence excitation. We report here on the details of the conversion of the Nikon C1 (product released November 2001) with two channels of nondescanned detection of two-photon-excited fluorescence. One of the goals of the design was to utilize off-the-shelf components as much as possible to minimize the use of custom machining and electronics assembly. We also give some initial characterization of the imaging properties of the system.
Ridsdale
Andrew
A
Neuroscience Unit, Ottawa Health Research Institute, 725 Parkdale Avenue, Ottawa, Canada. aridsdale@ohri.ca
Micu
Ileana
I
Stys
Peter K
PK
D004581:Electronics; D004867:Equipment Design; D018613:Microscopy, Confocal / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D017785:Photons