title: Characterisation of Shiga toxin-producing Escherichia coli (STEC) isolated from seafood and beef.
abstract: Shiga toxin-producing Escherichia coli (STEC) strains isolated in Mangalore, India, were characterised by bead-enzyme-linked immunosorbent assay (bead-ELISA), Vero cell cytotoxicity assay, PCR and colony hybridisation for the detection of stx1 and stx2 genes. Four strains from seafood, six from beef and one from a clinical case of bloody diarrhoea were positive for Shiga toxins Stx1 and Stx2 and also for stx1and stx2 genes. The seafood isolates produced either Stx2 alone or both Stx1 and Stx2, while the beef isolates produced Stx1 alone. The stx1 gene of all the beef STEC was found to be of recently reported stx1c type. All STEC strains and one non-STEC strain isolated from clam harboured EHEC-hlyA. Interestingly, though all STEC strains were negative for eae gene, two STEC strains isolated from seafood and one from a patient with bloody diarrhoea possessed STEC autoagglutinating adhesion (saa) gene, recently identified as a gene encoding a novel autoagglutinating adhesion.
authors.affiliation: Department of Fishery Microbiology, University of Agricultural Sciences, College of Fisheries, Mangalore 575 002, India.
authors.forename: H S
authors.initials: HS
authors.lastname: Kumar
authors.forename: Indrani
authors.initials: I
authors.lastname: Karunasagar
authors.forename: I
authors.initials: I
authors.lastname: Karunasagar
authors.forename: Tsukamoto
authors.initials: T
authors.lastname: Teizou
authors.forename: Kensuke
authors.initials: K
authors.lastname: Shima
authors.forename: Shinji
authors.initials: S
authors.lastname: Yamasaki
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics; D000818:Animals; D002417:Cattle; D002460:Cell Line; D002522:Chlorocebus aethiops; D003967:Diarrhea / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity / Q000502:physiology; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics; D006460:Hemolysin Proteins / Q000235:genetics; D006801:Humans; D008460:Meat / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D017747:Seafood / Q000382:microbiology*; D022622:Shiga Toxin 1 / Q000235:genetics / Q000378:metabolism / Q000633:toxicity; D022641:Shiga Toxin 2 / Q000235:genetics / Q000378:metabolism / Q000633:toxicity; D014709:Vero Cells; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; C439650:SAA protein, E coli; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D037521:Virulence Factors