Hyperthermophilic and salt-dependent formyltransferase from Methanopyrus kandleri.
Methanopyrus kandleri is a hyperthermophilic methanogenic archaeon, which grows on H(2) and CO(2) as its sole energy source. Its growth temperature optimum is 98 degrees C. One of the interesting characteristics of this archaeon is its high intracellular salt content. The organism has been reported to contain the trianionic cDPG (cyclic 2,3-diphosphoglycerate) and K+ at concentrations of 1.1 and 3 M, respectively. Reflecting the high cellular salt concentration, the enzymes in this organism are adapted not only to high temperature but also to high salt concentrations. The formyltransferase from M. kandleri was characterized extensively with respect to thermo- and halophilicity. The crystal structure of the formyltransferase at 1.73 A shows the enzyme to be composed of four identical subunits of molecular mass 32 kDa. The formyltransferase is thermostable and active only at relatively high concentrations of potassium phosphate (1 M) or other salts with strongly hydrated anions (strong salting-out salts). Potassium phosphate and potassium cDPG were found to be equivalent in activating and stabilizing the enzyme. At low concentrations of these salts, the enzyme is inactive and thermolabile. It was shown by equilibrium sedimentation analysis that the enzyme is in a monomer/dimer/tetramer equilibrium, the equilibrium constant being dependent on the concentration of salts: the higher oligomeric species increase with increasing salt concentrations. Evidence was provided that the monomer is both inactive and thermolabile. Experiments using a mutation which is directed to break surface ion pairs between two dimers indicated that dimerization is required for activity and tetramerization leads to thermostability.
Shima
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S
Max-Planck-Institut für terrestrische Mikrobiologie and Fachbereich Biologie, Philipps-Universität, Karl-von-Frisch Strasse, 35043 Marburg, Germany. shima@staff.uni-marburg.de
Thauer
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RK
Ermler
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U
D001105:Archaea / Q000201:enzymology*; D002245:Carbon Dioxide; D002455:Cell Division; D018360:Crystallography, X-Ray; D019281:Dimerization; D004305:Dose-Response Relationship, Drug; D019877:Hydroxymethyl and Formyl Transferases / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D009154:Mutation; D010710:Phosphates / Q000737:chemistry / Q000494:pharmacology; D011188:Potassium / Q000737:chemistry; D017680:Potassium Compounds / Q000737:chemistry / Q000494:pharmacology; D017433:Protein Structure, Secondary; D012492:Salts / Q000494:pharmacology; D013696:Temperature
D010710:Phosphates; D017680:Potassium Compounds; D012492:Salts; D002245:Carbon Dioxide; C013216:potassium phosphate; D019877:Hydroxymethyl and Formyl Transferases; D011188:Potassium