Protective effects of dietary soy isoflavones against UV-induced skin-aging in hairless mouse model.
OBJECTIVE
This study investigated the anti-aging effects of dietary isoflavones on photoaged hairless mouse skin.


METHODS
Female hairless mice were administered soy isoflavone extract orally and irradiated with UV light for four weeks. The effects of the isoflavones on the skin appearance, collagen deposition and epidermal thickness in the UV-damaged mouse skin were measured using bioengineering and histochemical methods. In addition, the influence of the isoflavones on the collagen metabolism in the UVB-irradiated human skin fibroblasts was also investigated.


RESULTS
In the isoflavone treated group, the skin had a better appearance and less wrinkling than that of the control group. Additionally, the amount of collagen deposition was higher in the isoflavone group. In the human fibroblast cells, the amount of procollagen de novo synthesized did not increase after isoflavone treatment and/or UV irradiation. However, the increase in the expression of the metalloproteinases (MMPs) as a result of UV irradiation was suppressed by the isoflavone treatment.


CONCLUSIONS
It appears that isoflavones had an anti-aging effect on the UV-damaged hairless mice model, which is partly due to the inhibitory effects on UV-induced MMP-1 expression and the subsequent collagen degradation.
Kim
Sun-Young
SY
Pharmaceutical & Health Research Institute, AmorePacific Corporation R&D Center, Yongin, Kyounggi, Korea.
Kim
Su-Jong
SJ
Lee
Jin-Young
JY
Kim
Wan-Gi
WG
Park
Won-Seok
WS
Sim
Young-Chul
YC
Lee
Sang-Jun
SJ
D000284:Administration, Oral; D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D007150:Immunohistochemistry; D007529:Isoflavones / Q000494:pharmacology*; D045726:Metalloproteases / Q000378:metabolism; D051379:Mice; D008812:Mice, Hairless; D011897:Random Allocation; D012867:Skin / Q000187:drug effects / Q000473:pathology* / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects
D007529:Isoflavones; D003094:Collagen; D045726:Metalloproteases