title: Construction of recombinant Escherichia coli strains for production of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate).
abstract: Plastic wastes constitute a worldwide environmental problem, and the demand for biodegradable plastics has become high. One of the most important characteristics of microbial polyesters is that they are thermoplastic with environmentally degradable properties. In this study, pUC19/PHA was cloned and transformed into three different Escherichia coli strains. Among the three strains that were successfully expressed in the production of polyhydroxyalkanoates (PHA), E. coli HMS174 had the highest yield in the production of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (P[HB-HV]). The cell dry weight and PHA content of recombinant HMS174 reached as high as 10.27 g/L and 43% (w/w), respectively, in fed-batch fermentor culture. The copolymer of PHA, P(HB-HV), was found in the cells, and the biopolymers accumulated were identified and analyzed by gas chromatography, proton nuclear magnetic resonance spectroscopy, and differential scanning calorimetry. We demonstrated clearly that the E. coli host for PHA production has to be carefully selected to obtain a high yield. The results obtained indicated that a superior E. coli with high PHA production can be constructed with a desirable ratio of P(HB-HV), which has potential applications in industry and medicine.
authors.affiliation: Open Laboratory of Chirotechnology of the Institute of Molecular Technology for Drug Discovery & Synthesis, Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Hong Kong, China.
authors.forename: Kin-Ho
authors.initials: KH
authors.lastname: Law
authors.forename: Pui-Ling
authors.initials: PL
authors.lastname: Chan
authors.forename: Wai-Sum
authors.initials: WS
authors.lastname: Lau
authors.forename: Yin-Chung
authors.initials: YC
authors.lastname: Cheng
authors.forename: Yun-Chung
authors.initials: YC
authors.lastname: Leung
authors.forename: Wai-Hung
authors.initials: WH
authors.lastname: Lo
authors.forename: Hugh
authors.initials: H
authors.lastname: Lawford
authors.forename: Hoi-Fu
authors.initials: HF
authors.lastname: Yu
mesh_terms: D001673:Biodegradation, Environmental; D001709:Biotechnology / Q000379:methods*; D002152:Calorimetry, Differential Scanning; D002244:Carbon / Q000737:chemistry; D002849:Chromatography, Gas; D004926:Escherichia coli / Q000737:chemistry* / Q000378:metabolism*; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D010957:Plasmids / Q000378:metabolism; D010969:Plastics; D011091:Polyesters / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D000094122:Polyhydroxybutyrates
chemical_list: D002244:Carbon; D010969:Plastics; D011091:Polyesters; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates