Concanavalin A chromatography coupled to two-dimensional gel electrophoresis improves protein expression studies of the serum proteome.
In the present study, we show a simple method to analyse human serum proteins using Concanavalin A (Con A) chromatography coupled to two-dimensional gel electrophoresis. Serum samples were separated into two fractions, one mainly containing non-glycosylated and O-glycosylated proteins and the other enriched in N-glycosylated proteins. Both fractions were subjected to two-dimensional gel electrophoresis, and the obtained maps were analysed. The method presented here improves the resolution of the serum proteome, increasing the number of visualized spots over two times and allowing the detection of proteins with lower abundance in serum. We have proved the feasibility of the method comparing the N-glycoprotein fraction of serum from donors and colorectal cancer (CRC) patients.
Rodríguez-Piñeiro
Ana María
AM
Departamento de Bioquímica, Genética e Inmunología, Facultad de Ciencias, As Lagoas Marcosende, s/n 36200 Vigo, Spain.
Ayude
Daniel
D
Rodríguez-Berrocal
Francisco Javier
FJ
Páez de la Cadena
María
M
D002853:Chromatography, Liquid / Q000379:methods*; D003208:Concanavalin A / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male
D003208:Concanavalin A