[Detection of human parvovirus B19 nonstructural protein DNA by nested- polymerase chain reaction in pregnant women].
OBJECTIVE
To explore the sensitivity and specificity of the new nested-polymerase chain reaction (nested-PCR) assay in the detection of human parvovirus B19 (HPV B19) nonstructural protein DNA and the applicability of this technique in detecting the gravida infected with parvovirus B19.


METHODS
Evaluating the new nested-polymerase chain reaction assay by routine process, and compared with the general nested-PCR in detecting structural protein DNA. Then, the two techniques were used to detect human parvovirus B19 structural protein and nonstructural protein DNA in gravida serum and pregnant tissue of 30 cases.


RESULTS
The sensitivity of the new method was 0.005 fg which was similar to the general nested-polymerase chain reaction assay, and the detection results of other viruses and human genome DNA were all negative. The positive rates of structural protein DNA and nonstructural protein DNA in gravida sera were 26.7% and 33.3%, respectively. And the difference was non significant in statistics. At the same time, both of these two positive rates in placenta and villus were 26.7%.


CONCLUSIONS
The new nested-polymerase chain reaction assay was a sensitive, specific and convenient method in detecting human parvovirus B19 infection, and the detection of nonstructural protein DNA paves the way for the following study on the disease mechanisms of B19.
Qiao
Fu-yuan
FY
Department of Obstetrics and Gynecology, The Affiliated Tongji Hospital, Tongji college, HUST, Wuhan 430030, China.
Huang
Yong-mei
YM
Li
Zeng-qing
ZQ
Liu
Hai-yi
HY
Tang
Hong-ju
HJ
D005260:Female; D006801:Humans; D016732:Parvovirus B19, Human / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D012680:Sensitivity and Specificity; D017361:Viral Nonstructural Proteins / Q000235:genetics*
D017361:Viral Nonstructural Proteins