Protein-protein interactions of proteins from the ESAT-6 family of Mycobacterium tuberculosis.
In the present study, we demonstrate that, in analogy with the genes encoding ESAT-6 and CFP-10, the genes rv0287 and rv0288 from the ESAT-6 gene family are cotranscribed. Using Western-Western blotting and protein-print overlay methodologies, we demonstrate that ESAT-6 and CFP-10, as well as the protein pair Rv0288/Rv0287, interact pairwise in a highly specific way. Most notably, the ESAT-6 proteins interact directly with Rv3873, a possible cell envelope component of the ESAT-6 secretion pathway.
Okkels
Limei Meng
LM
Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, DK-2300, Denmark. okkels@mpiib-berlin.mpg.de
Andersen
Peter
P
D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C412542:CFP 6 protein, Mycobacterium tuberculosis; C117940:CFP-10 protein, Mycobacterium tuberculosis