title: Neuropoietin, a new IL-6-related cytokine signaling through the ciliary neurotrophic factor receptor.
abstract: A structural profile-based computational screen was used to identify neuropoietin (NP), a new cytokine. The np gene is localized in tandem with the cardiotrophin-1 gene on mouse chromosome 7. NP shares structural and functional features with ciliary neurotrophic factor (CNTF), cardiotrophin-1, and cardiotrophin-like cytokine. It acts through a membrane receptor complex comprising CNTF receptor-alpha component (CNTFRalpha), gp130, and leukemia inhibitory factor receptor to activate signal transducer and activator of transcription 3 signaling pathway. NP is highly expressed in embryonic neuroepithelia. Strikingly, CNTFRalpha, but not its alternate ligands, CNTF and cardiotrophin-like cytokine, is expressed at the same developmental stages. NP is also observed in retina and to a lesser extent in skeletal muscle. Moreover, NP could sustain the in vitro survival of embryonic motor neurons and could increase the proliferation of neural precursors when associated to epidermal growth factor and fibroblast growth factor 2. Thus, NP is a new ligand for CNTFRalpha, with important implications for murine nervous system development.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale U564, Centre Hospitalier Universitaire d'Angers, 4 Rue Larrey, 49033 Angers, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Derouet
authors.forename: François
authors.initials: F
authors.lastname: Rousseau
authors.forename: Fabienne
authors.initials: F
authors.lastname: Alfonsi
authors.forename: Josy
authors.initials: J
authors.lastname: Froger
authors.forename: Jacques
authors.initials: J
authors.lastname: Hermann
authors.forename: Fabien
authors.initials: F
authors.lastname: Barbier
authors.forename: David
authors.initials: D
authors.lastname: Perret
authors.forename: Caroline
authors.initials: C
authors.lastname: Diveu
authors.forename: Catherine
authors.initials: C
authors.lastname: Guillet
authors.forename: Laurence
authors.initials: L
authors.lastname: Preisser
authors.forename: Agnès
authors.initials: A
authors.lastname: Dumont
authors.forename: Maud
authors.initials: M
authors.lastname: Barbado
authors.forename: Alain
authors.initials: A
authors.lastname: Morel
authors.forename: Odile
authors.initials: O
authors.lastname: deLapeyrière
authors.forename: Hugues
authors.initials: H
authors.lastname: Gascan
authors.forename: Sylvie
authors.initials: S
authors.lastname: Chevalier
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA; D006801:Humans; D015850:Interleukin-6 / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D020801:Receptor, Ciliary Neurotrophic Factor / Q000378:metabolism / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D015850:Interleukin-6; D020801:Receptor, Ciliary Neurotrophic Factor; C486482:neuropoietin, mouse; D004247:DNA