Comparison of virulence of various hantaviruses related to hemorrhagic fever with renal syndrome in newborn mouse model.
The virulence of hantaviruses that are antigenically related but have different genetic characteristics from the prototype of hantavirus, Hantaan (HTN) virus, was examined in newborn mice. The H5 and B78 strains of the Amur (AMR) genotype, the Bao14 strain of the Far East (FE) genotype, and the 76-118 strain of HTN virus were inoculated subcutaneously (1focus-forming unit; FFU) into newborn mice. All of the AMR and FE genotype viruses inoculated mice were died by 16 days post-infection (dpi) and 21 dpi, respectively, while 50% of the HTN virus inoculated mice survived until 30 dpi. The AMR and FE genotype viruses inoculated mice had high viral titers in the lung (1.3x10(6) to 1.3x10(8) FFU/gram [g] tissue) , brain (2.1x10(7) to 1.2x10(9) FFU/g tissue), and kidney(2.5x10(5) to 1.6x10(7) FFU/g tissue), and showed a detectable level of antibodies (titers 1:16-1:32) at 14 dpi. In contrast, the HTN virus infected mice had viruses only in the lungs at low titers (1.1-5.3x10(5) FFU/g tissue). Observations of body-weight changes revealed that the AMR and FE genotype viruses inoculated mice had lower growth rates than the HTN virus inoculated mice. These data suggest that the AMR and FE genotype viruses are more virulent than the HTN virus in newborn mice.
Lokugamage
Kumari
K
Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Kariwa
Hiroaki
H
Lokugamage
Nandadeva
N
Iwasa
Masahiro
M
Hagiya
Tomohiro
T
Araki
Koichi
K
Tachi
Atsushi
A
Mizutani
Tetsuya
T
Yoshimatsu
Kumiko
K
Arikawa
Jiro
J
Iwasaki
Takuya
T
Takashima
Ikuo
I
D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000097:blood; D001921:Brain / Q000821:virology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006476:Orthohantavirus / Q000472:pathogenicity*; D018778:Hantavirus Infections / Q000097:blood / Q000821:virology*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000097:blood / Q000821:virology*; D007668:Kidney / Q000821:virology; D008168:Lung / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D013047:Specific Pathogen-Free Organisms; D016019:Survival Analysis; D014774:Virulence
D000914:Antibodies, Viral