Liquid chromatographic-tandem mass spectrometric method for the quantitation of huperzine A in dog plasma.
A rapid and sensitive LC-MS-MS method for the determination of huperzine A in dog plasma using huperzine B as internal standard has been developed and validated. The analyte and internal standard were extracted from plasma using n-hexane-dichloromethane-2-propanol (300:150:15, v/v/v), chromatographed on a C(18) column (5 microm, 50 mm x 4.6 mm i.d.) with a mobile phase consisting of acetonitrile-methanol-10mM ammonium acetate (35:40:25, v/v/v), and detected using a tandem mass spectrometer with a TurboIonSpray ionization interface. The run time was only 2 min. The assay was linear over the concentration range 0.05-20 ng/ml and intra- and inter-day precision over this range were <5.3% with good accuracy. The limit of detection in plasma was 0.01 ng/ml. The method was successfully applied to define plasma concentration-time curves of huperzine A in dogs after the last dose of an intramuscular injection (10 microg/kg per day for 15 days) of a sustained-release formulation of huperzine A.
Wang
Yingwu
Y
Research Center for Drug Metabolism, College of Life Science, Jilin University, Jiefang Road 123, Changchun 130023, China.
Chu
Dafeng
D
Gu
Jingkai
J
Fawcett
J Paul
JP
Wu
Yi
Y
Liu
Wanhui
W
D000470:Alkaloids; D000818:Animals; D004285:Dogs; D013058:Mass Spectrometry; D018696:Neuroprotective Agents / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012717:Sesquiterpenes / Q000097:blood*
D000470:Alkaloids; D018696:Neuroprotective Agents; D012717:Sesquiterpenes; C050426:huperzine A