title: Non-replicating mucosal and systemic vaccines: quantitative and qualitative differences in the Ag-specific CD8(+) T cell population in different tissues.
abstract: Directed dissemination of Ag-specific CD8(+) T cells to infected organs or cancerous tissues is a prerequisite for optimal immunotherapy. Ag-specific CD8(+) T cells were quantitated in systemic and mucosal tissues after nasal, rectal, or cutaneous immunization with CTL epitope peptide and the adjuvant cholera toxin (CT). Mucosal and cutaneous immunization induced Ag-specific CD8(+) lymphocytes that were detectable in both mucosal and systemic compartments, suggesting a less strict distribution pattern than that known for B cells. However, optimal localization, activation and phenotype of these cells correlated with the route of immunization. In accordance with this observation, protection against a mucosal challenge with a virus expressing the CTL epitope was superior in mucosally-immunized animals.
authors.affiliation: Department of Microbiology and Immunology, Faculty of Health Sciences and the Cancer Research Center, Ben Gurion University of the Negev, Beer Sheva 84105, Israel.
authors.forename: Udi
authors.initials: U
authors.lastname: Qimron
authors.forename: Lada
authors.initials: L
authors.lastname: Paul
authors.forename: Erez
authors.initials: E
authors.lastname: Bar-Haim
authors.forename: Noga
authors.initials: N
authors.lastname: Bloushtain
authors.forename: Lea
authors.initials: L
authors.lastname: Eisenbach
authors.forename: Herman F
authors.initials: HF
authors.lastname: Staats
authors.forename: Angel
authors.initials: A
authors.lastname: Porgador
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000918:Antibody Specificity / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002772:Cholera Toxin / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D018928:Immunity, Mucosal / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D009092:Mucous Membrane / Q000276:immunology; D010641:Phenotype; D014018:Tissue Distribution; D014612:Vaccines / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000939:Epitopes; D014612:Vaccines; D002772:Cholera Toxin