Effect of 3' terminal codon pairs with different frequency of occurrence on the expression of cat gene in Escherichia coli.
In a previous study, we have identified four types of 3' terminal codon pairs depending on their frequency of occurrence in the Escherichia coli genome: overrepresented, moderately represented, underrepresented, and missing. In this study, the influence of eight codon pairs belonging to these four groups on the efficiency of chloramphenicol acetyltransferase ( cat) gene expression in E. coli is examined. Our results show that the missing codon pairs CCU:UAG (Pro:Stop) and CCC:UAG (Pro:Stop) had decreasing effect, whereas another missing pair CCU:AGG (Pro:Arg) had an opposite effect on the yield of CAT protein in comparison with the wild-type cat gene.
Boycheva
Svetlana S
SS
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria.
Bachvarov
Boris I
BI
Berzal-Heranz
Alfredo
A
Ivanov
Ivan G
IG
D001426:Bacterial Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics*; D003062:Codon; D018388:Codon, Terminator; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D014158:Transcription, Genetic; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D003062:Codon; D018388:Codon, Terminator; D012329:RNA, Bacterial; D012333:RNA, Messenger; D015500:Chloramphenicol O-Acetyltransferase