Development of an immunosensor for the detection of vitellogenin using impedance spectroscopy.
A simple and direct immunosensor for the determination of carp (Carassius auratus) vitellogenin (Vtg), a female-specific protein, has been proposed based on an antibody-captured conducting polymer-coated electrode. The monoclonal antibody specific to carp (C. auratus) Vtg was immobilized by covalent coupling to the carboxylic acid group on the polymer. The antibody immobilization and antibody-antigen interaction have been demonstrated by means of quartz crystal microbalance and impedance spectroscopic techniques. The impedance change occurred at the sensor surface due to the specific immuno-interaction was utilized to determine Vtg. The sensor showed high selectivity and sensitive response to Vtg in a buffered medium without redox probe. Vtg was determined in the linear range from 1.0 to 8.0 microg/l with the standard deviation of +/-0.13 (n =3) and the detection limit was determined to be 0.42 microg/l. This method was applied to the determination of Vtg in real male and female carp (C. auratus) serum samples.
Darain
Farzana
F
Department of Chemistry and Center for Innovative Bio-Physio Sensor Technology, Pusan National University, Busan 609-735, South Korea.
Park
Deog-Su
DS
Park
Jang-Su
JS
Shim
Yoon-Bo
YB
D000906:Antibodies / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation*; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000295:instrumentation; D013057:Spectrum Analysis; D013696:Temperature; D014819:Vitellogenins / Q000032:analysis* / Q000276:immunology
D000906:Antibodies; D014819:Vitellogenins