Meat consumption patterns and preparation, genetic variants of metabolic enzymes, and their association with rectal cancer in men and women.
Meat consumption, particularly of red and processed meat, is one of the most thoroughly studied dietary factors in relation to colon cancer. However, it is not clear whether meat, red meat, heterocyclic amines (HCA), or polycyclic aromatic hydrocarbons (PAH) are associated with the risk for rectal cancer. Rectal cancer cases (n = 952) and controls (n = 1205) from Utah and Northern California were recruited from a population-based case-control study between September 1997 and February 2002. Detailed in-person interviews regarding lifestyle, medical history, and diet were conducted. DNA was extracted from peripheral lymphocytes obtained from whole-blood samples, and glutathione S-transferase (GST)M1 enzyme and N-acetyl transferase (NAT)2 enzyme genotypes were assessed. Although energy and cholesterol intakes were higher among cases than controls, adjustment for confounders accounted for the differences. Increased consumption of well-done red meat [odds ratio (OR) 1.33 95% CI 0.98, 1.79] was associated with an (P = 0.04) increase in risk for rectal cancer among men. The mutagen index, calculated on the bases of reported amount, doneness, and method of cooking meat, was also positively but not significantly (P = 0.24) associated with risk of rectal cancer for men (OR 1.37 95% CI 0.98, 1.92). NAT2-imputed phenotype and GSTM1 did not consistently modify rectal cancer risk associated with meat intake. These data suggest that mutagens such as HCA that form when meat is cooked may be culpable substances in rectal cancer risk, not red meat itself.
Murtaugh
Maureen A
MA
Department of Family and Preventive Medicine, University of Utah, Salt Lake City, UT 84101, USA. mmurtaugh@hrc.utah.edu
Ma
Khe-Ni
KN
Sweeney
Carol
C
Caan
Bette J
BJ
Slattery
Martha L
ML
D000328:Adult; D000368:Aged; D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D016022:Case-Control Studies; D003296:Cooking / Q000379:methods; D004032:Diet; D005260:Female; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006358:Hot Temperature; D006801:Humans; D008297:Male; D008460:Meat / Q000032:analysis; D008875:Middle Aged; D009153:Mutagens / Q000032:analysis; D010641:Phenotype; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects / Q000032:analysis; D012004:Rectal Neoplasms / Q000453:epidemiology* / Q000209:etiology; D012307:Risk Factors
D001191:Arylamine N-Acetyltransferase; D005982:Glutathione Transferase; D009153:Mutagens; D011084:Polycyclic Aromatic Hydrocarbons; C478900:NAT2 protein, human