Preparation of PLLA membranes with different morphologies for culture of MG-63 Cells.
In this work, poly L-lactide (PLLA) membranes with different morphologies were prepared and the equilibrium phase diagram of membrane formation system of ethanol, methylene chloride, and PLLA was studied. Based on the phase diagram, particulate and porous membranes, dominated by crystallization and liquid-liquid demixing, respectively, could be prepared by changing the PLLA concentration of casting solution. In addition, in vitro interaction of MG-63 osteosarcoma cells and PLLA membranes with dense, porous and particulate morphologies was investigated. It was found that the particulate membrane not only could improve cell adhesion and growth, but also could upregulate the osteoblastic phenotype. Therefore, the PLLA membrane with particulate morphology satisfies the biomaterial requirement necessary for temporary scaffold to transplanted osteoblasts and provides a means for the architectural design of more complex tissue-engineered systems.
Liu
Hwa-Chang
HC
Department of Orthopedic Surgery, National University Hospital, Taipei 100, Taiwan, ROC.
Lee
I-Chi
IC
Wang
Jyh-Horng
JH
Yang
Shu-Hua
SH
Young
Tai-Horng
TH
D000293:Adolescent; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division; D002460:Cell Line; D048429:Cell Size; D002470:Cell Survival; D006801:Humans; D008422:Materials Testing; D008567:Membranes, Artificial; D008968:Molecular Conformation; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011091:Polyesters; C033616:poly(lactide)