The role of the sympathetic nervous system in postasphyxial intestinal hypoperfusion in the pre-term sheep fetus.
Asphyxia in utero in pre-term fetuses is associated with evolving hypoperfusion of the gut after the insult. We examined the role of the sympathetic nervous system (SNS) in mediating this secondary hypoperfusion. Gut blood flow changes were also assessed during postasphyxial seizures. Preterm fetal sheep at 70% of gestation (103-104 days, term is 147 days) underwent sham asphyxia or asphyxia induced by 25 min of complete cord occlusion and fetuses were studied for 3 days afterwards. Phentolamine (10 mg bolus plus 10 mg h(-1)i.v.) or saline was infused for 8 h starting 15 min after the end of asphyxia or sham asphyxia. Phentolamine blocked the fall in superior mesenteric artery blood flow (SMABF) after asphyxia and there was a significant decrease in MAP for the first 3 h of infusion (33 +/- 1.6 mmHg versus vehicle 36.7 +/- 0.8 mmHg, P < 0.005). During seizures SMABF fell significantly (8.3 +/- 2.3 versus 11.4 +/- 2.7 ml min(-1), P < 0.005), and SMABF was more than 10% below baseline for 13.0 +/- 1.7 min per seizure (versus seizure duration of 78.1 +/- 7.2 s). Phentolamine was associated with earlier onset of seizures (5.0 +/- 0.4 versus 7.1 +/- 0.7 h, P < 0.05), but no change in amplitude or duration, and prevented the fall in SMABF. In conclusion, the present study confirms the hypothesis that postasphyxial hypoperfusion of the gut is strongly mediated by the SNS. The data highlight the importance of sympathetic activity in the initial elevation of blood pressure after asphyxia and are consistent with a role for the mesenteric system as a key resistance bed that helps to maintain perfusion in other, more vulnerable systems.
Quaedackers
Josine S
JS
Department of Anatomy, The University of Auckland, Private Bag 92019 Auckland, New Zealand.
Roelfsema
Vincent
V
Heineman
Erik
E
Gunn
Alistair J
AJ
Bennet
Laura
L
D000818:Animals; D001237:Asphyxia / Q000503:physiopathology*; D001784:Blood Gas Analysis; D001786:Blood Glucose / Q000378:metabolism; D001794:Blood Pressure / Q000502:physiology; D004569:Electroencephalography; D004576:Electromyography; D005260:Female; D005333:Fetus / Q000098:blood supply / Q000502:physiology*; D006340:Heart Rate, Fetal / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000098:blood supply*; D007511:Ischemia / Q000503:physiopathology; D019344:Lactic Acid / Q000097:blood; D010477:Perfusion; D011247:Pregnancy; D011942:Receptors, Adrenergic, alpha / Q000502:physiology; D012039:Regional Blood Flow / Q000502:physiology; D012640:Seizures / Q000503:physiopathology; D012756:Sheep; D013152:Splanchnic Circulation / Q000502:physiology; D013564:Sympathetic Nervous System / Q000503:physiopathology*; D014470:Umbilical Cord / Q000502:physiology; D014655:Vascular Resistance / Q000502:physiology
D001786:Blood Glucose; D011942:Receptors, Adrenergic, alpha; D019344:Lactic Acid