[Effect of clonidine on rat's behavior response and spinal nitric oxide in formalin test].
OBJECTIVE
To assess the effect of Clonidine on SD rat's behavior response and spinal nitric oxide in the formalin test.


METHODS
The effects of Clonidine (Clo), L-arginine(L-Arg), and NG-nitro-L-arginine (L-NAME) on the flinching and licking time of rats in the formalin test were assessed using the model of formalin-induced hyperalgia.


RESULTS
The flinching and licking time of group Clo was shorter (38.60 +/- 17.34) s/5 min than that of group Formalin(155.41 +/- 22.04) s/5 min. Pre-administration of L-Arg could potentiate the Clonidine-induced response; pre-administration of L-NAME could reduce the Clonidine-induced response.


CONCLUSIONS
Spinal nitric oxide release might be one of the antinociception mechanisms of Clonidine in the formalin test.
Lin
Xue-mei
XM
Department of Anesthesiology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Xu
Hong-wei
HW
Wang
Quan-yun
QY
Huang
Wei
W
Yang
Bang-xiang
BX
Zhu
Lin
L
D000700:Analgesics / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D003000:Clonidine / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism*
D000700:Analgesics; D009569:Nitric Oxide; D003000:Clonidine