title: Chromatography paper strip method for collection, transportation, and storage of rotavirus RNA in stool samples.
abstract: We developed a novel method that uses sodium dodecyl sulfate-EDTA-treated chromatography paper strips to collect unconcentrated fresh stool samples. After the paper strips were stored for 4 months at room temperature, rotavirus RNA could be successfully amplified by using reverse transcriptase PCR. The use of filter paper strips as a specimen support allows (self-)collection of stool samples by untrained persons. Diarrheal stool samples from remote areas can be stored and transported to a central diagnostic laboratory without the need for freezers or special shipping conditions. This convenient and inexpensive rotavirus sample collection system can be of use in epidemiological surveillance studies and vaccine trials.
authors.affiliation: Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, University of Leuven, Belgium.
authors.forename: Mustafizur
authors.initials: M
authors.lastname: Rahman
authors.forename: Truus
authors.initials: T
authors.lastname: Goegebuer
authors.forename: Karolien
authors.initials: K
authors.lastname: De Leener
authors.forename: Piet
authors.initials: P
authors.lastname: Maes
authors.forename: Jelle
authors.initials: J
authors.lastname: Matthijnssens
authors.forename: Goutam
authors.initials: G
authors.lastname: Podder
authors.forename: Tasnim
authors.initials: T
authors.lastname: Azim
authors.forename: Marc
authors.initials: M
authors.lastname: Van Ranst
mesh_terms: D002854:Chromatography, Paper; D003967:Diarrhea / Q000821:virology; D004492:Edetic Acid; D005243:Feces / Q000821:virology*; D006801:Humans; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D011934:Reagent Strips; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000821:virology; D012967:Sodium Dodecyl Sulfate; D013048:Specimen Handling / Q000379:methods*
chemical_list: D012367:RNA, Viral; D011934:Reagent Strips; D012967:Sodium Dodecyl Sulfate; D004492:Edetic Acid