The chirality of a four-way helical junction in RNA.
We have used a novel electrophoretic approach to determine the chirality of a four-way helical junction in RNA. From our experiments we conclude that the handedness of the helical junction in the complete ribozyme is opposite to that found in the free junction and is therefore constrained by the interaction between the unpaired loops of the ribozyme.
Goody
Terry A
TA
Cancer Research UK Nucleic Acid Structure Research Group, MSI/WTB Complex, The University of Dundee, Dundee DD1 5EH, UK.
Lilley
David M J
DM
Norman
David G
DG
D001483:Base Sequence; D004586:Electrophoresis; D008958:Models, Molecular; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D016337:RNA, Catalytic / Q000737:chemistry; D012330:RNA, Double-Stranded / Q000737:chemistry*; D013237:Stereoisomerism
D016337:RNA, Catalytic; D012330:RNA, Double-Stranded