Solution structures of the inactive and BeF3-activated response regulator CheY2.
The chemotactic signalling chain to the flagellar motor of Sinorhizobium meliloti features a new type of response regulator, CheY2. CheY2 activated by phosphorylation (CheY2-P) controls the rotary speed of the flagellar motor (instead of reversing the sense of rotation), and it is efficiently dephosphorylated by phospho-retrotransfer to the cognate kinase, CheA. Here, we report the NMR solution structures of the Mg(2+)-complex of inactive CheY2, and of activated CheY2-BeF(3), a stable analogue of CheY2-P, to an overall root mean square deviation of 0.042 nm and 0.027 nm, respectively. The 14 kDa CheY2 protein exhibits a characteristic open (alpha/beta)(5) conformation. Modification of CheY2 by BeF(3)(-) leads to large conformational changes of the protein, which are in the limits of error identical with those observed by phosphorylation of the active-centre residue Asp58. In BeF(3)-activated CheY2, the position of Thr88-OH favours the formation of a hydrogen bond with the active site, Asp58-BeF(3), similar to BeF(3)-activated CheY from Escherichia coli. In contrast to E.coli, this reorientation is not involved in a Tyr-Thr-coupling mechanism, that propagates the signal from the incoming phosphoryl group to the C-terminally located FliM-binding surface. Rather, a rearrangement of the Phe59 side-chain to interact with Ile86-Leu95-Val96 along with a displacement of alpha4 towards beta5 is stabilised in S.meliloti. The resulting, activation-induced, compact alpha4-beta5-alpha5 surface forms a unique binding domain suited for specific interaction with and signalling to a rotary motor that requires a gradual speed control. We propose that these new features of response regulator activation, compared to other two-component systems, are the key for the observed unique phosphorylation, dephosphorylation and motor control mechanisms in S.meliloti.
Riepl
Hubert
H
Lehrstuhl für Genetik, Universität Regensburg, D-93040 Regensburg, Germany.
Scharf
Birgit
B
Schmitt
Rüdiger
R
Kalbitzer
Hans Robert
HR
Maurer
Till
T
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D001608:Beryllium / Q000737:chemistry*; D001665:Binding Sites; D002633:Chemotaxis / Q000502:physiology; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism; D005407:Flagella / Q000378:metabolism; D005459:Fluorides / Q000737:chemistry*; D008958:Models, Molecular; D020409:Molecular Motor Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010766:Phosphorylation; D011487:Protein Conformation; D016415:Sequence Alignment; D015398:Signal Transduction / Q000502:physiology; D016962:Sinorhizobium meliloti / Q000737:chemistry* / Q000378:metabolism
D001426:Bacterial Proteins; D020409:Molecular Motor Proteins; C035982:beryllium fluoride; D001608:Beryllium; D005459:Fluorides