title: Spatial distribution of marine crenarchaeota group I in the vicinity of deep-sea hydrothermal systems.
abstract: Distribution profiles of marine crenarchaeota group I in the vicinity of deep-sea hydrothermal systems were mapped with culture-independent molecular techniques. Planktonic samples were obtained from the waters surrounding two geographically and geologically distinct hydrothermal systems, and the abundance of marine crenarchaeota group I was examined by 16S ribosomal DNA clone analysis, quantitative PCR, and whole-cell fluorescence in situ hybridization. A much higher proportion of marine crenarchaeota group I within the microbial community was detected in deep-sea hydrothermal environments than in normal deep and surface seawaters. The highest proportion was always obtained from the ambient seawater adjacent to hydrothermal emissions and chimneys but not from the hydrothermal plumes. These profiles were markedly different from the profiles of epsilon-Proteobacteria, which are abundant in the low temperatures of deep-sea hydrothermal environments.
authors.affiliation: Subground Animalcule Retrieval Project, Frontier Research System for Extremophiles, Japan Marine Science & Technology Center, Yokosuka 237-0061, Japan. kent@jamstec.go.jp
authors.forename: Ken
authors.initials: K
authors.lastname: Takai
authors.forename: Hanako
authors.initials: H
authors.lastname: Oida
authors.forename: Yohey
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Hisako
authors.initials: H
authors.lastname: Hirayama
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nakagawa
authors.forename: Takuro
authors.initials: T
authors.lastname: Nunoura
authors.forename: Fumio
authors.initials: F
authors.lastname: Inagaki
authors.forename: Kenneth H
authors.initials: KH
authors.lastname: Nealson
authors.forename: Koki
authors.initials: K
authors.lastname: Horikoshi
mesh_terms: D001483:Base Sequence; D019615:Crenarchaeota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D019641:DNA, Archaeal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D017753:Ecosystem; D006358:Hot Temperature; D017404:In Situ Hybridization, Fluorescence; D019156:Indian Ocean; D007564:Japan; D008969:Molecular Sequence Data; D010140:Pacific Ocean; D010802:Phylogeny; D010933:Plankton / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012623:Seawater / Q000382:microbiology*
chemical_list: D019641:DNA, Archaeal; D004275:DNA, Ribosomal