title: The cytotoxic activity of the total alkaloids isolated from different parts of Solanum pseudocapsicum.
abstract: The total alkaloid fractions of the methanolic extracts of the leaves, ripe fruits, roots, seeds and stem of Solanum pseudocapsicum were subjected to in-vitro cytotoxicity, short-term toxicity and long-term survival studies. All the five fractions exhibited potent activity. The total alkaloid fraction of leaves was found to be the most potent. The HT-29 cell line was the most sensitive to the fractions. The cytotoxic concentration (CTC(50)) values for all these fractions ranged between 0.39-0.91, 0.68-2.8, 0.92-3.56, 4.05-8.2, 3.28-5.65 and 0.95-5.55 microg/ml, respectively for HT-29, RD-228, A-549, HEp-2, B(16)F(10) and Vero cell lines. In short-term toxicity studies, the fractions showed 50% viability at 93-128 microg/ml for DLA cells and 141-189 microg/ml for human lymphocytes. In the long-term survival studies on the cell lines RD-228, HEp-2 and Vero, cells retained their regenerative capacities at concentrations below 8 microg/ml. The total alkaloids of the plant, especially from the leaves merit further investigations to identify the active constituents in animal models.
authors.affiliation: J.S.S. College of Pharmacy, Rocklands, OOTACAMUND-643 001, Tamil Nadu, India. vijayanp4@rediffmail.com
authors.forename: Pottekkad
authors.initials: P
authors.lastname: Vijayan
authors.forename: Preethi
authors.initials: P
authors.lastname: Vijayaraj
authors.forename: Prashanth Haranahalli Chandrappa
authors.initials: PH
authors.lastname: Setty
authors.forename: Raghu Chandrashekhar
authors.initials: RC
authors.lastname: Hariharpura
authors.forename: Ashok
authors.initials: A
authors.lastname: Godavarthi
authors.forename: Shrishailappa
authors.initials: S
authors.lastname: Badami
authors.forename: Dhanaraj Sokkalingam
authors.initials: DS
authors.lastname: Arumugam
authors.forename: Suresh
authors.initials: S
authors.lastname: Bhojraj
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology* / Q000633:toxicity; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology; D018514:Plant Structures / Q000737:chemistry; D019657:Solanaceae / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts