Intensive swimming exercise-induced oxidative stress and reproductive dysfunction in male wistar rats: protective role of alpha-tocopherol succinate.
In the present study, 30 male rats (age 3 mos, Wt 128.6 +/- 3.7 g) were randomly divided into Control group (CG), Experimental group (EG), and Supplemented group (SG), 10 per group. An exercise protocol (3 hrs swimming per day, 5 days a week for 4 weeks) was followed in EG and SG, with no exercise in CG. In SG, alpha-tocopherol succinate was injected sub-cutaneously at a dose of 50 mg x kg(- 1) per body weight per day. After 4 weeks of exercise, significant diminutions (p < 0.05) were noted in somatic indices of testes and accessory sex organs; seminiferous tubuler diameter (STD); testicular (delta (5), 3 beta-hydroxysteroid dehydrogenase delta(5), 3 beta-HSD), 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) activities; plasma levels of testosterone (T), luteinizing hormone (LH); preleptotine spermatocytes (pLSc), mid-pachytene spermatocytes (mPSc), and Stage 7 spermatids (7 Sd); testicular alpha-tocopherol and glutathione (GSH) content; superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and glutathione-s-transferase (GST) activities in EG when compared to CG. Moreover, a significant elevation (p < 0.05) in malondialdehyde (MDA) was found in testes of EG compared to CG. No significant alteration was noted in body weight among the groups. Co-administration of alpha-tocopherol succinate restored the above parameters. Intensive swimming exercise-induced oxidative stress causes dysfunction in the male reproductive system, which can be protected by alpha-tocopherol succinate.
Manna
Indranil
I
Human Performance Lab, Sports Authority of India, New Delhi, India.
Jana
Kuladip
K
Samanta
Prabhat Kumar
PK
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013550:Swimming / Q000502:physiology*; D013737:Testis / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000097:blood / Q000172:deficiency; D024505:Tocopherols; D014810:Vitamin E / Q000008:administration & dosage* / Q000031:analogs & derivatives*
D000975:Antioxidants; D014810:Vitamin E; D013739:Testosterone; D024505:Tocopherols