title: Progression and specificity of protein oxidation in the life cycle of Arabidopsis thaliana.
abstract: Protein carbonylation is an irreversible oxidative process leading to a loss of function of the modified proteins, and in a variety of model systems, including worms, flies, and mammals, carbonyl levels gradually increase with age. In contrast, we report here that in Arabidopsis thaliana an initial increase in protein oxidation during the first 20 days of the life cycle of the plant is followed by a drastic reduction in protein carbonyls prior to bolting and flower development. Protein carbonylation prior to the transition to flowering targets specific proteins such as Hsp70, ATP synthases, the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), and proteins involved in light harvesting/energy transfer and the C2 oxidative photosynthetic carbon cycle. The precipitous fall in protein carbonyl levels is due to the specific reduction in the levels of oxidized proteins rather than an overall loss of chlorophyll and Rubisco associated with the senescence syndrome. The results are discussed in light of contemporary theories of aging in animals.
authors.affiliation: Department of Cell and Molecular Biology, Göteborg University, Box 462, 405 30 Göteborg, Sweden.
authors.forename: Elin
authors.initials: E
authors.lastname: Johansson
authors.forename: Olof
authors.initials: O
authors.lastname: Olsson
authors.forename: Thomas
authors.initials: T
authors.lastname: Nyström
mesh_terms: D025181:ATP Synthetase Complexes / Q000737:chemistry; D017360:Arabidopsis / Q000378:metabolism*; D002244:Carbon / Q000737:chemistry; D002736:Chloroplasts / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry; D008928:Mitochondria / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
chemical_list: D018840:HSP70 Heat-Shock Proteins; D010940:Plant Proteins; D002244:Carbon; D025181:ATP Synthetase Complexes; D010100:Oxygen