[Effect of triester glycerol type of plasticizers on release of albumin from biodegradable polymer matrices].
Bovine serum albumin was heterogeneously dispersed in the terpolymer of DL-lactic acid, glycolic acid, and mannitol. The terpolymeric carrier was plasticized by triacetin, tributyrin, or a mixture of triacetin with tricaprylin. Matrices were prepared by mixing a melt of the carrier with the plasticizer and albumin. Liberation was tested in 1/15 mol.l-1 phosphate buffer pH 7.4 and took place in two stages--the burst and the stage of the continual process. The burst represented 30% to 90% of liberated albumin. The second, continual stage began on day 4 of liberation and within ten days maximally 10% of the total amount of albumin was released. The course of liberation was most markedly influenced by the parameters of the oligoester carrier, its molecular weight, and the degree of branching. The total extent of albumin liberation was influenced partly by its irreversible adsorption on the carrier, partly by its polymerization. The extent of adsorption and polymerization of albumin was increased with the molecular weight of the carrier and decreased with the increasing degree of its branching. Albumin liberation was positively influenced by the presence of plasticizers in matrices, triacetin being demonstrated as the best one.
Kladnícková
I
I
Katedra farmaceutické technologie Farmaceutické fakulty Univerzity Karlovy, Hradec Králové. 95kli4155@faf.cuni.cz
Klein
T
T
Dittrich
M
M
D001673:Biodegradation, Environmental; D002210:Caprylates; D004337:Drug Carriers; D010968:Plasticizers; D011108:Polymers; D012710:Serum Albumin, Bovine; D014215:Triacetin; D014280:Triglycerides
D002210:Caprylates; D004337:Drug Carriers; D010968:Plasticizers; D011108:Polymers; D014280:Triglycerides; D012710:Serum Albumin, Bovine; C003637:tricaprylin; C005830:tributyrin; D014215:Triacetin