title: Set1 is required for meiotic S-phase onset, double-strand break formation and middle gene expression.
abstract: The Set1 protein of Saccharomyces cerevisiae is a histone methyltransferase (HMTase) acting on lysine 4 of histone H3. Inactivation of the SET1 gene in a diploid leads to a sporulation defect. We have studied various processes that take place during meiotic differentiation in set1delta diploid cells. The absence of Set1 leads to a delay of meiotic S-phase onset, which reflects a defect in DNA replication initiation. The timely induction of meiotic DNA replication does not require the Set1 HMTase activity, but depends on the SET domain. In addition, set1delta displays a severe impairment of the DNA double-strand break formation, which is not only the consequence of the replication delay. Transcriptional profiling experiments show that the induction of middle meiotic genes, but not of early meiotic genes, is affected by the loss of Set1. In contrast to meiotic replication, the transcriptional induction of the middle meiotic genes appears to depend on the methylation of H3-K4. Our results unveil multiple roles of Set1 in meiotic differentiation and distinguish between HMTase-dependent and -independent Set1 functions.
authors.affiliation: LISM, CNRS, Marseille Cedex, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Sollier
authors.forename: Waka
authors.initials: W
authors.lastname: Lin
authors.forename: Christine
authors.initials: C
authors.lastname: Soustelle
authors.forename: Karsten
authors.initials: K
authors.lastname: Suhre
authors.forename: Alain
authors.initials: A
authors.lastname: Nicolas
authors.forename: Vincent
authors.initials: V
authors.lastname: Géli
authors.forename: Christophe
authors.initials: C
authors.lastname: de La Roche Saint-André
mesh_terms: D004249:DNA Damage / Q000235:genetics; D004261:DNA Replication / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D018816:Genes, cdc; D011495:Histone-Lysine N-Methyltransferase / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D047468:Immunoprecipitation; D020411:Oligonucleotide Array Sequence Analysis; D010957:Plasmids / Q000235:genetics; D016196:S Phase / Q000502:physiology*; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D013172:Spores, Fungal / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D004268:DNA-Binding Proteins; D006657:Histones; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D011495:Histone-Lysine N-Methyltransferase; C411250:SET1 protein, S cerevisiae