Chemo-affinity of titania for the column-switching HPLC analysis of phosphopeptides.
A column-switching high-performance liquid chromatography (HPLC) system for the determination of phosphopeptides has been developed. The method is based on the selective adsorption of phosphopeptides on a titania (TiO2) precolumn and successive HPLC separation of the phosphopeptides on an anion-exchange column with a UV detector (215 nm). The recoveries of phosphopeptides were tested using authentic phosphopeptides [Gln-Ile-Ser(p)-Val-Arg, Ile-Ser(p)-Val-Arg and Lys-Gln-Ile-Ser(p)-Val-Arg] at an injection amount of 1 microg. The recoveries were 74.3, 79.6, and 82.6%, respectively, while the corresponding dephosphopeptides were not retained on the titania precolumn.
Sano
Akira
A
Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
Nakamura
Hiroshi
H
D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D010748:Phosphopeptides / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D014025:Titanium / Q000032:analysis*
D010748:Phosphopeptides; D014025:Titanium