title: No deterioration after 13 years in a stability study of a rabbit brain, plain, thromboplastin, RBT 1010, in rubber-stoppered ampoules.
abstract: RBT 1010, a rabbit brain thromboplastin, plain, was prepared at the Thrombosis Reference Centre, Withington Hospital, Manchester, in 1989. The batch has been stored at -20 degrees C, in rubber-stoppered ampoules, for 13 years. The material was unchanged after this time. This was confirmed in a stability study, in which the reagent was used to test the prothrombin times of a panel of plasmas stored in liquid nitrogen, one from a normal volunteer and two from stable anticoagulated patients. RBT 1010 was also tested in calibrations, according to World Health Organization recommendations, against the reference thromboplastin preparations CRM 149S and RBT 90.
authors.affiliation: Haematology Research Unit, Department of Clinical Laboratory Medicine, Wythenshawe Hospital, Manchester M23 9LT, UK. ken.stevenson@smuht.nwest.nhs.uk
authors.forename: K J
authors.initials: KJ
authors.lastname: Stevenson
authors.forename: S
authors.initials: S
authors.lastname: Craig
authors.forename: L
authors.initials: L
authors.lastname: Goodman
authors.forename: F B
authors.initials: FB
authors.lastname: Kanyike
mesh_terms: D000818:Animals; D001921:Brain; D015925:Cryopreservation / Q000592:standards; D005612:Freeze Drying / Q000592:standards; D011517:Prothrombin Time / Q000592:standards; D011817:Rabbits; D012015:Reference Standards; D012408:Rubber; D013048:Specimen Handling / Q000295:instrumentation / Q000592:standards*; D013925:Thromboplastin / Q000592:standards*; D013997:Time Factors
chemical_list: D012408:Rubber; D013925:Thromboplastin