Expression of flap endonuclease-1 during meiosis in a basidiomycete, Coprinus cinereus.
In the basidiomycete Coprinus cinereus (C. cinereus), which shows a highly synchronous meiotic cell cycle, the meiotic prophase I cells demonstrate flap endonuclease-1 activity. To investigate its role during meiosis, we isolated a C. cinereus cDNA homolog of flap endonuclease-1 (CcFEN-1), 1377bp in length with the open reading frame (ORF) encoding a predicted molecular mass of 51 kDa. At amino-acid residues Glu276-Pro345, a specific inserted sequence composed of 70 amino acids rich in polar forms was found to exist, without sequence identity to other eukaryotic FEN-1 or the polar amino acid rich sequences found in C. cinereus PCNA and C. cinereus DNA ligase IV, although the lengths and percentages of polar amino acids were similar. Northern hybridization analysis indicated CcFEN-1 to be expressed not only in the pre-meiotic S phase but also in meiotic prophase I. The roles of CcFEN-1 during meiosis are discussed.
Yamaguchi
Taiki
T
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba-ken 278-8510, Japan.
Namekawa
Satoshi H
SH
Hamada
Fumika N
FN
Kasai
Nobuyuki
N
Nara
Takayuki
T
Watanabe
Kei
K
Iwabata
Kazuki
K
Ishizaki
Takashi
T
Ishii
Satomi
S
Koshiyama
Akiyo
A
Inagaki
Sachiyo
S
Kimura
Seisuke
S
Sakaguchi
Kengo
K
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry / Q000235:genetics; D003302:Coprinus / Q000201:enzymology* / Q000235:genetics*; D000072481:DNA Ligase ATP; D011088:DNA Ligases / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D004271:DNA, Fungal / Q000737:chemistry / Q000302:isolation & purification; D045585:Flap Endonucleases / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D008540:Meiosis / Q000502:physiology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D010802:Phylogeny; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics; D011418:Prophase / Q000235:genetics / Q000502:physiology; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D016196:S Phase / Q000235:genetics / Q000502:physiology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D000596:Amino Acids; D018076:DNA, Complementary; D004271:DNA, Fungal; D018809:Proliferating Cell Nuclear Antigen; D012331:RNA, Fungal; D012333:RNA, Messenger; D045585:Flap Endonucleases; D011088:DNA Ligases; D000072481:DNA Ligase ATP