Transient expression of MDR-1/P-glycoprotein in a model of partial cortical devascularization.
1. MDR-1 gene product confer to expressing cells the multidrug resistance phenotype to a broad range of drugs and xenobiotics. 2. It is known that different stress signals are able to induce MDR-1 expression through different promoters. 3. In a rat model of ischemia by partial cortical devascularization we studied the expression profile and the cellular localization of MDR-1 after 1, 3, 7, 14 and 28 days post lesion (DPL). 4. Using two different antibody clones we found that MDR-1 is expressed in cortical and striatal neurons ipsilateral to the devascularizing lesion, starting at 1DPL, showing a maximum at 7DPL to be thereafter reduced until undetectable levels by 28DPL. 5. MDR-1 expression may be defining a neuronal subset with a particular pharmacological profile.
Ramos
Alberto Javier
AJ
Facultad de Ciencias Biomédicas, Universidad Austral, Pilar, Buenos Aires, Argentina.
Lazarowski
Alberto
A
Villar
Marcelo J
MJ
Brusco
Alicia
A
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active / Q000502:physiology; D002545:Brain Ischemia / Q000378:metabolism* / Q000503:physiopathology; D002462:Cell Membrane / Q000378:metabolism; D002540:Cerebral Cortex / Q000098:blood supply / Q000378:metabolism* / Q000503:physiopathology; D004195:Disease Models, Animal; D018450:Disease Progression; D018432:Drug Resistance, Multiple / Q000235:genetics; D008297:Male; D017072:Neostriatum / Q000378:metabolism / Q000503:physiopathology; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time / Q000502:physiology
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1