Elucidation of retention mechanisms on hypercrosslinked polystyrene used as column packing material for high-performance liquid chromatography.
Establishing of basic retention mechanisms was considered the key target during the development of new column packing materials. To extract, from an appropriate retention data matrix on hypercrosslinked polystyrene Chromalite 5HGN, certain factors that can be brought in an obvious correspondence with known retention mechanisms, the principal component analysis (PCA) was applied. The approach was used to elucidate the adsorption properties of the above novel HPLC packing. Besides HPLC, knowledge of retention mechanisms helps to reveal perspective application area for the hypercrosslinked polystyrene-type materials in solid-phase extraction (SPE) and low-pressure preparative LC.
Sychov
C S
CS
Institute of Organo-Element Compounds, Russian Academy of Science, Vavilov Street 28, Moscow 119991, Russia.
Ilyin
M M
MM
Davankov
V A
VA
Sochilina
K O
KO
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D011137:Polystyrenes / Q000737:chemistry*
D011137:Polystyrenes