title: Validation of a confirmatory method for the determination of sulphonamides in muscle according to the European Union regulation 2002/657/EC.
abstract: A simple multiresidue method is described for assaying 10 sulphonamides (SAs) (sulfadiazine, sulfathiazole, sulfapyridine, sulfamerazine, sulfamethazine, sulfamonomethoxine, sulfachlorpyridazine, sulfamethoxazole, sulfaquinoxaline and sulfadimethoxine) in muscle samples. Samples were prepared by homogenizing the tissue, extracting with ethyl acetate and cleaning up with a cation-exchange solid-phase extraction (SPE) column. The detection of analytes was achieved by HPLC-diode array detection (DAD) at 270 nm. The procedure was validated according to the European Union regulation 2002/657/EC determining specificity, decision limit, detection capability, trueness and precision. The results of validation process demonstrate that the method is suitable for application in European Union statutory veterinary drug residue surveillance programmes.
authors.affiliation: Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, via Salvemini, Perugia 1-06126, Italy. i.pecorelli@pg.izs.it
authors.forename: Ivan
authors.initials: I
authors.lastname: Pecorelli
authors.forename: Rita
authors.initials: R
authors.lastname: Bibi
authors.forename: Laura
authors.initials: L
authors.lastname: Fioroni
authors.forename: Roberta
authors.initials: R
authors.lastname: Galarini
mesh_terms: D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005062:European Union; D009132:Muscles / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013449:Sulfonamides / Q000032:analysis*
chemical_list: D013449:Sulfonamides