title: [Pilot study of neonatal rat cardiac myocytes cultured for three-dimensional modeling in simulated microgravity].
abstract: OBJECTIVE
  To study three-dimensional culturing methods of neonatal rat cardiac myocytes in simulated microgravity.
  
  
  METHODS
  Neonatal rat primary cardiac myocytes were separated and seeded into polylactic acid scaffolds, stirred in spinner flasks for 24 hours, and then moved into rotary cell culture system for three-dimensional culture. The growth of cardiac myocytes was observed under inverted phase contrast microscope, scanning electron microscope and transmission electron microscope, and metabolic assay was assessed by MTT assay.
  
  
  RESULTS
  Cardiac myocytes with sustained metabolic activity attached to the polylactic acid scaffolds, extended and confluenced. Pulsations of PLA-cardiac myocytes was found in some areas.
  
  
  CONCLUSIONS
  The rotary cell culture system is suitable to develop neonatal rat cardiac myocytes culturing for three-dimensional modeling.
authors.affiliation: Institute of Space Medico-Engineering, Beijing, P. R. China 100094.
authors.forename: Fen
authors.initials: F
authors.lastname: Yang
authors.forename: Ying-hui
authors.initials: YH
authors.lastname: Li
authors.forename: Jie-lin
authors.initials: JL
authors.lastname: Nie
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002470:Cell Survival; D002478:Cells, Cultured; D019344:Lactic Acid; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000648:ultrastructure; D011091:Polyesters; D011108:Polymers; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering; D018474:Weightlessness Simulation
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)