HLA-Ig-based artificial antigen-presenting cells: setting the terms of engagement.
Recent advances in molecular medicine have shown that soluble MHC-multimers can be valuable tools for both the stimulation of as well as the analysis of antigen-specific T cells in vitro. In this review, we describe the use of dimeric major histocompatibility complexes, HLA-Ig, to visualize antigen-specific T cells as well as their potential to stimulate immune responses as part of an artificial antigen-presenting cell (aAPC). The use of HLA-Ig-based aAPC represents an exciting new approach to generate antigen-specific CTL for adoptive immunotherapy that helps to overcome many of the obstacles associated with limitations in current approaches to adoptive immunotherapy.
Oelke
Mathias
M
Department of Pathology and Medicine, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA. bmpe5@cs.com
Schneck
Jonathan P
JP
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D015789:HLA-A2 Antigen / Q000276:immunology*; D006801:Humans; D007136:Immunoglobulins / Q000737:chemistry / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D046911:Macromolecular Substances; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000941:Antigens; D015789:HLA-A2 Antigen; D007136:Immunoglobulins; D046911:Macromolecular Substances