title: Alteration of plasma total F2-isoprostanes before and after hemodialysis in end-stage renal disease patients.
abstract: F(2)-isoprostanes are derived in vivo principally from the following: (1) the formation of positional peroxyl radicals of arachidonic acid, (2) endocyclization to prostaglandin G(2)-like structures, and (3) reduction to PGF(2)-like compounds. F(2)-isoprostanes have been proposed as biomarkers of lipid peroxidation, oxidative stress status, and the oxidation of low-density lipoprotein (LDL). Using gas chromatography-ion trap-mass spectrometry, we studied how hemodialysis (HD) affects plasma total F(2)-isoprostanes. We examined the plasma total F(2)-isoprostanes in end-stage renal disease (ESRD) patients, before HD, after HD, between HD, and in control subjects. Plasma concentrations of total F(2)-isoprostanes were significantly higher in the after HD ESRD patients than the before hemodialysis ESRD patients (P < 0.05). There is no difference between before HD ESRD patients and normal controls. Moreover, a positive or negative correlation was seen between LDL and plasma total F(2)-isoprostanes (P < 0.001), and between age and plasma total F(2)-isoprostanes (P < 0.001). This study indicates HD treatment may be the major contributor of oxidative stress in ESRD patients.
authors.affiliation: Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, P.O. Box 131, Seoul 130-650, South Korea.
authors.forename: Kyung Mee
authors.initials: KM
authors.lastname: Kim
authors.forename: Byung Hwa
authors.initials: BH
authors.lastname: Jung
authors.forename: Ki-Jung
authors.initials: KJ
authors.lastname: Paeng
authors.forename: Sang Wook
authors.initials: SW
authors.lastname: Kim
authors.forename: Bong Chul
authors.initials: BC
authors.lastname: Chung
mesh_terms: D016718:Arachidonic Acid / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D028441:F2-Isoprostanes / Q000097:blood*; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000503:physiopathology / Q000628:therapy; D015227:Lipid Peroxidation / Q000502:physiology; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology; D011237:Predictive Value of Tests; D006435:Renal Dialysis
chemical_list: D015415:Biomarkers; D028441:F2-Isoprostanes; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D016718:Arachidonic Acid