[Induction of transforming growth factor-beta 1 and dentin non-collagen proteins on tissue engineering pulp].
OBJECTIVE
To study the influence of transforming growth factor-beta 1 (TGF-beta 1), dentin non-collagen proteins (dNCPs) and their complex on tissue engineering pulp system.


METHODS
Collagen I and dentin powder were used to construct the system of pulp cells in 3-dimensional culture, dentin powder was added in the gel. The tissue engineering pulp were divided TGF-beta 1 group, dNCPs group, TGF-beta 1/dNCPs group and control group. After 3, 6 and 14 days, the appearance and the differentiation of pulp cells were observed by HE staining and immunohistochemical staining respectively.


RESULTS
Collagen I could form netted collagen gel construction. Growing condition of pulp cells in gel was similar to that of pulp cells in vivo. After the TGF-beta 1 and dNCPs were added, the pulp cells had some characteristics of odontoblasts and had unilateral cell process after culture 6 days. Pulp cells arranged with parallel columnar and form dentin-pulp-like complex after 14 days. Immunohistochemical staining showed dentin salivary protein (DSP) began to express in some cells. The number of positive cell was most in the TGF-beta 1 group. No positive cells were detected in the control group.


CONCLUSIONS
The transforming growth factor-beta 1 and non-collagen proteins can stimulate the pulp cells to transform into odontoblasts to some extent, which promote the formation of tissue engineering pulp.
Nie
Xin
X
Department of Oralpathology, Qindu Hospital, Fourth Military Medical University, Xi'an, Shaanxi, P. R. China 710032.
Jin
Yan
Y
Zhang
Chun-yan
CY
D000818:Animals; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D003810:Dentinogenesis / Q000187:drug effects*; D005109:Extracellular Matrix / Q000378:metabolism; D007150:Immunohistochemistry; D009804:Odontoblasts / Q000187:drug effects* / Q000378:metabolism; D010750:Phosphoproteins / Q000494:pharmacology*; D023822:Tissue Engineering; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D010750:Phosphoproteins; D016212:Transforming Growth Factor beta; C017630:phosphophoryn