Mutagenicities of 8-hydroxyguanine and 2-hydroxyadenine produced by reactive oxygen species.
Oligodeoxyribonucleotides containing 8-hydroxyguanine and 2-hydroxyadenine, purine lesions produced in cells by reactive oxygen species, were synthesized and inserted into vector DNAs to introduce each lesion at a predetermined site. The manipulated DNAs were transfected into living cells, and the mutants induced by each DNA lesion were collected and analyzed. In addition, the mutations induced by damaged DNA precursors with the two oxidized purine bases were studied by the use of chemically synthesized nucleoside triphosphates. In this review article, the author summarizes the mutagenic potentials of the two oxidized purine bases, by focusing on experiments examined by the author and his collaborators.
Kamiya
Hiroyuki
H
Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan. hirokam@pharm.hokudai.ac.jp
D000818:Animals; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004249:DNA Damage; D005822:Genetic Vectors; D006147:Guanine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism*; D016296:Mutagenesis; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000235:genetics; D010084:Oxidation-Reduction; D011685:Purine Nucleotides / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000737:chemistry / Q000378:metabolism*
D009838:Oligodeoxyribonucleotides; D011685:Purine Nucleotides; D017382:Reactive Oxygen Species; C024829:8-hydroxyguanine; D006147:Guanine; D004247:DNA; C008183:isoguanine