Identification of a P2-related prophage remnant locus of Photorhabdus luminescens encoding an R-type phage tail-like particle.
Analysis of the Photorhabdus luminescens genome sequence revealed that the pts region is related to the tail synthesis gene core of the P2 phage. The pts locus encodes a DNA invertase homologue. PCR-RFLP analysis showed the two potential tail fiber regions of the pts locus present DNA inversions. Electron microscopy revealed a phage tail-like particle, related to the R-type family and named R-photorhabdicin, in the culture supernatant of P. luminescens. Mass spectrometry analysis of two sub-units of R-photorhabdicin revealed that they are encoded by the pts locus. The role of this P2-related prophage remnant in the Photorhabdus genome is discussed.
Gaudriault
Sophie
S
Laboratoire EMIP, Université Montpellier II, IFR122, Institut National de la Recherche Agronomique (UMR 1133), 34095 Montpellier Cedex 5, France. sgaudriault@crit.univ-montp2fr
Thaler
Jacques-Olivier
JO
Duchaud
Eric
E
Kunst
Frank
F
Boemare
Noël
N
Givaudan
Alain
A
D017901:Bacteriophage P2 / Q000201:enzymology / Q000235:genetics* / Q000648:ultrastructure; D008854:Microscopy, Electron; D020637:Photorhabdus / Q000235:genetics* / Q000821:virology*; D039002:Prophages / Q000201:enzymology / Q000235:genetics / Q000648:ultrastructure; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D043324:beta-Fructofuranosidase / Q000235:genetics* / Q000378:metabolism
D043324:beta-Fructofuranosidase