title: Autonomous function of the amino-terminal inhibitory domain of TAF1 in transcriptional regulation.
abstract: The general transcription factor TFIID is composed of TATA-binding protein (TBP) and 14 TBP-associated factors (TAFs). TFIID mediates the transcriptional activation of a subset of eukaryotic promoters. The N-terminal domain (TAND) of TAF1 protein (Taf1p) inhibits TBP by binding to its concave and convex surfaces. This study examines the role of the TAND in transcriptional regulation and tests whether the TAND is an autonomous regulator of TBP. The TAND binds to and regulates TBP function when it is fused to the amino or carboxy terminus of Taf1p, the amino or carboxy terminus of Taf5p, or the amino terminus of Taf11p. However, a carboxy-terminal fusion of the TAND and Taf11p is not compatible with several other TAF proteins, including Taf1p, in the TFIID complex. These results indicate that there is no or minimal geometric constraint on the ability of the TAND to function normally in transcriptional regulation as long as TFIID assembly is secured.
authors.affiliation: Division of Molecular and Cellular Biology, Graduate School of Integrated Science, Yokohama City University, Yokohama 230-0045, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Takahata
authors.forename: Koji
authors.initials: K
authors.lastname: Kasahara
authors.forename: Masashi
authors.initials: M
authors.lastname: Kawaichi
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Kokubo
mesh_terms: D015966:Gene Expression Regulation, Fungal; D046911:Macromolecular Substances; D008957:Models, Genetic; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D035381:TATA-Binding Protein Associated Factors / Q000235:genetics / Q000378:metabolism*; D035362:Transcription Factor TFIID / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D046911:Macromolecular Substances; D029701:Saccharomyces cerevisiae Proteins; C106785:TAF1 protein, S cerevisiae; C105463:TAF11 protein, S cerevisiae; C438897:TAF5 protein, S cerevisiae; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID