Production of wheat-Leymus racemosus chromosome addition lines.
We produced ten wheat-Leymus racemosus chromosome addition lines. Eight chromosomes (A, C, F, H, I, J, k, and l) were recovered as disomic additions and two (E and n) as monosomic. Screening of the addition lines was done by fluorescence in situ hybridization using several repetitive sequences as probes, which allowed us to identify different L. racemosus chromosomes and find many aberrant L. racemosus chromosomes. RFLP analysis revealed partial conservation of homology between L. racemosus and wheat chromosomes, depending on the homologous groups. Chromosomes A and l belonged to group 2, chromosomes C and I to group 5, and chromosome k to group 6. Chromosomes H and J were a mixture of groups 1, 3, and 7, chromosome n of groups 3 and 7, and chromosomes E and F were of group 4 and others. Comparison of our addition lines with other addition lines showed large cytological differences.
Kishii
Masahiro
M
Laboratory of Plant Genetics and Breeding Science, Faculty of Agriculture, Tottori University, 680-8553 Tottori, Japan.
Yamada
Toyomi
T
Sasakuma
Tetsuo
T
Tsujimoto
Hisashi
H
D015139:Blotting, Southern; D032461:Chromosomes, Plant / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D030821:Plants, Genetically Modified; D006109:Poaceae / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D026801:Synteny / Q000235:genetics; D014908:Triticum / Q000235:genetics*