title: Fibroblast response to a controlled nanoenvironment produced by colloidal lithography.
abstract: It is thought that by understanding how cells respond to topography, that better tissue engineering may be achievable. An important consideration in the cellular environment is topography. The effects of microtopography have been well documented, but the effects of nanotopography are less well known. Previously, methods of nanofabrication have been costly and time-consuming, but research by engineers, physicists, and chemists is starting to allow the production of nanostructures using low-cost techniques. In this report, nanotopography is specifically considered. Controlled patterns of 160 nm high nanocolumns were produced for in vitro cell culture using colloidal lithography. By studying cell adhesion with time and cytoskeletal (actin, tubulin, and vimentin) maturity, insight has been gained as to how fibroblasts adhere to these nanofeatures.
authors.affiliation: Centre for Cell Engineering, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, UK. m.dalby@bio.gla.ac.uk
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Dalby
authors.forename: Mathis O
authors.initials: MO
authors.lastname: Riehle
authors.forename: Duncan S
authors.initials: DS
authors.lastname: Sutherland
authors.forename: Hossein
authors.initials: H
authors.lastname: Agheli
authors.forename: Adam S G
authors.initials: AS
authors.lastname: Curtis
mesh_terms: D000199:Actins / Q000502:physiology; D001672:Biocompatible Materials; D002448:Cell Adhesion / Q000502:physiology; D003102:Colloids / Q000378:metabolism; D003599:Cytoskeleton / Q000502:physiology; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D036103:Nanotechnology; D019904:Polymethyl Methacrylate; D013997:Time Factors; D014746:Vimentin / Q000502:physiology; D016596:Vinculin / Q000502:physiology
chemical_list: D000199:Actins; D001672:Biocompatible Materials; D003102:Colloids; D014746:Vimentin; D016596:Vinculin; D019904:Polymethyl Methacrylate