New macroporous calcium phosphate glass ceramic for guided bone regeneration.
This work describes a method to obtain macroporous resorbable glass and glass ceramic scaffolds with controlled biodegradability for tissue engineering applications. The constructs consisted of glass and glass ceramics in the system P(2)O(5)-CaO-Na(2)O-TiO(2) and they were prepared by foaming a slurry of glass particles by addition of a H(2)O(2) solution, and subsequent sintering of the porous structures obtained. Different thermal treatments were applied to control the degree of devitrification of the glass. The resultant materials showed a porosity percentage between 40% and 55% with a wide variety of pores ranging from 20 to 500 microm in diameter as determined by SEM and Image Analysis. The resulting constructs were predominantly formed by a vitreous phase, although small amounts of calcium metaphosphate and pyrophosphates were detected by X-ray diffraction and Raman spectroscopy after the sintering process. The biological response was also evaluated by means of the MTT test, the material showed a non-cytotoxic effect.
Navarro
Melba
M
Biomedical Engineering Research Center (CREB), Department of Materials Science and Metallurgy, Technical University of Catalonia, ETSEIB, Avda Diagonal 647, Barcelona 08028, Spain. melba.navarro@upc.es
del Valle
Sergio
S
Martínez
Salvador
S
Zeppetelli
Stefania
S
Ambrosio
Luigi
L
Planell
Josep Anton
JA
Ginebra
Maria Pau
MP
D020341:Absorbable Implants; D001861:Bone Regeneration / Q000502:physiology*; D018786:Bone Substitutes / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival; D002516:Ceramics / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D016348:Osseointegration / Q000502:physiology; D010006:Osteoblasts / Q000166:cytology*; D016062:Porosity; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
D018786:Bone Substitutes; D002130:Calcium Phosphates; C020243:calcium phosphate