Requirement for an intact cytoskeleton for volume regulation in boar spermatozoa.
Osmotically induced cell swelling triggers a chain of events leading to a net loss of major cell ions and water, resulting in cell volume recovery, a process known as regulatory volume decrease (RVD). In many cell types, there is an evidence that the cytoskeleton may play a role in the initial sensing and transduction of the signal of volume change. In this study, we tested the hypothesis that an intact microfilament and microtubule network is required for volume response and RVD in boar sperm before and after capacitation treatment and whether addition of cytochalasin D and colchicine to the capacitation medium would affect volumetric behaviour. Capacitation is a series of cellular and molecular alterations that enable the spermatozoon to fertilize an oocyte. Cell volume measurements of washed sperm suspensions were performed electronically in Hepes-buffered saline solutions of 300 and 180 mosmol/kg. After exposure to hypoosmotic conditions, boar sperm showed initial swelling (up to 150% of initial volume within 5 min), which was subsequently partially reversed (to about 120-130% after 20 min). Treatment with cytochalasin D led to reduced initial swelling (1 micromol/l) and loss of RVD in washed sperm (1-10 micromol/l) and at the beginning of incubation under capacitating conditions (5 micromol/l). Short treatment with 500 micromol/l colchicine affected the volume regulatory ability in sperm under capacitating conditions but not in washed sperm. No significant differences in cell volume response were observed after subsequent addition of cytochalasin D and colchicine to the suspensions of sperm incubated for 3 h under capacitating conditions. However, the incubation under capacitating conditions in the presence of cytochalasin D led to improved volume regulation at the end of the incubation period (23%). The microfilament network appears to be important for volume regulation in washed boar spermatozoa while intact microtubules do not seem to be necessary for osmotically induced RVD. The changes in cytoskeleton microfilament organization during capacitation, possibly affecting the osmotically induced volume response, appear to occur at the later stages of capacitation, whereas changes in microtubules, related to volume regulatory ability, may be programmed within the first stages of capacitation.
Petrunkina
A M
AM
Institute for Reproductive Medicine, School of Veterinary Medicine Hanover, Bünteweg 15, 30559 Hanover, Germany. Ana.Petrunkina@tiho-hannover.de
Hebel
M
M
Waberski
D
D
Weitze
K F
KF
Töpfer-Petersen
E
E
D008841:Actin Cytoskeleton / Q000648:ultrastructure; D000818:Animals; D048429:Cell Size; D003078:Colchicine / Q000494:pharmacology; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000648:ultrastructure*; D008297:Male; D008870:Microtubules / Q000648:ultrastructure; D009994:Osmolar Concentration; D013075:Sperm Capacitation / Q000187:drug effects / Q000502:physiology*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology* / Q000187:drug effects; D013552:Swine / Q000502:physiology*
D015638:Cytochalasin D; D003078:Colchicine