Comparative study of smoke condensates from 1R4F cigarettes that burn tobacco versus ECLIPSE cigarettes that primarily heat tobacco in the SENCAR mouse dermal tumor promotion assay.
Numerous chemical and toxicological studies indicate that smoke from ECLIPSE, a cigarette that primarily heats rather than burns tobacco, is simplified and reduced in specific chemicals believed to be associated with smoking-related diseases, and demonstrates reduced smoke toxicity and biological activity in vitro when compared to conventional tobacco burning cigarettes. These data led to the hypothesis that cigarette smoke condensate (CSC) from ECLIPSE should have lower tumorigenicity than 1R4F condensate in the SENCAR mouse dermal tumor promotion assay. Female SENCAR mice were initiated with a single topical application of 7,12-dimethylbenz[a]anthracene (DMBA) followed by promotion with ECLIPSE or 1R4F CSC. Dermal application of 10, 20, or 40 mg ECLIPSE or 1R4F CSC three times/week for 29 weeks did not alter body weights, survival or other indicators of subchronic toxicity. In DMBA-initiated mice, there were significant increases in both the number of microscopically confirmed tumor-bearing animals and total number of microscopically confirmed dermal tumors at all 1R4F CSC doses and the high-dose ECLIPSE CSC. However, the number of ECLIPSE tumor-bearing animals were reduced 83%, 93% and 67% at the low-, mid- and high-doses, respectively, compared to the 1R4F. Similarly, the total number of dermal tumors was reduced 91%, 94% and 87% at the low-, mid- and high-dose, respectively, compared to the 1R4F CSC. ECLIPSE CSC demonstrated dramatic reductions in dermal tumor promotion potential compared to 1R4F CSC.
Meckley
Daniel R
DR
Research and Development Department, R. J. Reynolds Tobacco Company, P.O. Box 1487, Winston-Salem, NC 27102-1487, USA. mackled@rjrt.com
Hayes
Johnnie R
JR
Van Kampen
K R
KR
Ayres
Paul H
PH
Mosberg
Arnold T
AT
Swauger
James E
JE
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000008:administration & dosage / Q000633:toxicity; D000287:Administration, Topical; D000818:Animals; D001681:Biological Assay; D002273:Carcinogens / Q000008:administration & dosage / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D005260:Female; D006358:Hot Temperature; D006801:Humans; D051379:Mice; D018632:Mice, Inbred SENCAR; D009152:Mutagenicity Tests; D009153:Mutagens / Q000008:administration & dosage / Q000009:adverse effects*; D012867:Skin / Q000187:drug effects*; D012878:Skin Neoplasms / Q000139:chemically induced* / Q000453:epidemiology / Q000473:pathology; D012906:Smoke / Q000009:adverse effects*; D014026:Nicotiana
D002273:Carcinogens; D009153:Mutagens; D012906:Smoke; D015127:9,10-Dimethyl-1,2-benzanthracene