title: Impact of feline zona pellucida glycoprotein B-derived synthetic peptides on in vitro fertilization of cat oocytes.
abstract: Although immunocontraception based on porcine zona pellucida (ZP) proteins is widely applied in many species, it is not suitable for cat contraception due to the lack of cross-reactivity. Since the first ZP gene expressed during oocyte growth in domestic cats is ZPB, we assumed that immunization with feline ZPB (fZPB)-derived synthetic peptides may cause irreversible infertility, which would be preferable in stray cats. Thus, the present study evaluated the immunogenicity and the contraceptive potential of synthetic fZPB peptides. Antigenic epitope sequences were detected via epitope mapping using specific rabbit anti-fZP antibodies. Six peptides representing the recognized epitopes were synthesized subsequently. Two out of six peptides (ZPB amino acid residue 130-149 = P3 and 175-193 = P6) cross-reacted with anti-fZP antiserum in dot blot analysis and ELISA. Coupled to BSA, both peptides were utilized to produce specific antibodies in rats. Despite several booster injections the antibody titers monitored by ELISA did not exceed 1:5000. Both rat antisera were tested for contraceptive potential in cat in vitro maturation/in vitro fertilization (IVF). Antiserum against peptide P3 significantly inhibited sperm binding and fertilization of cat oocytes in vitro (57.3% of sperm binding; 41.5% of fertilization), whereas the inhibition by anti-P6 was not significant. Pre-incubation of sperm cells with both peptides before IVF failed to affect either sperm binding or fertilization (22.3 +/- 3.7 sperm/egg vs 25.5 +/- 5.8 for P3 and 20.7 +/- 4.0 for P6, respectively). In conclusion, antibodies directed against one of the two identified antigenic determinants of fZPB inhibited sperm binding and IVF and therefore showed promising results as a contraceptive. However, the specific immune response and anti-fertile properties of this synthetic vaccine have to be examined in vivo to verify the suitability of its components.
authors.affiliation: Institute for Zoo and Wildlife Research, PF 601103, D-10252 Berlin, Germany. ringleb@izw-berlin.de
authors.forename: Jennifer
authors.initials: J
authors.lastname: Ringleb
authors.forename: Marlies
authors.initials: M
authors.lastname: Rohleder
authors.forename: Katarina
authors.initials: K
authors.lastname: Jewgenow
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D002415:Cats; D003269:Contraception, Immunologic / Q000662:veterinary*; D003429:Cross Reactions; D003470:Culture Media; D004527:Egg Proteins / Q000737:chemistry* / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D005260:Female; D005307:Fertilization in Vitro / Q000187:drug effects*; D008297:Male; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000276:immunology; D009865:Oocytes / Q000187:drug effects; D010455:Peptides / Q000276:immunology*; D051381:Rats; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000276:immunology; D013084:Sperm-Ovum Interactions / Q000187:drug effects; D000072216:Zona Pellucida Glycoproteins
chemical_list: D000906:Antibodies; D003470:Culture Media; D004527:Egg Proteins; D000939:Epitopes; D008562:Membrane Glycoproteins; D010455:Peptides; D011956:Receptors, Cell Surface; D000072216:Zona Pellucida Glycoproteins; C000606522:Zp4 protein, rat