title: Comparison of manual and automated methods for determining platelet counts in dogs with macrothrombocytopenia.
abstract: Platelet counts were performed in 43 Cavalier King Charles Spaniels (CKCS, a breed predisposed to macrothrombocytopenia) and in 10 control dogs using 3 automated systems and 3 manual methods (erythrocyte-lysing agents + counting chamber or evaluation of blood smear). Good correlations were found between platelet counts using all methods (all P < 0.0001; R2 = 0.71-0.85). Best correlations were found between the manual methods. Significantly larger platelets were found in CKCS with platelet count < or = 100,000/microl when compared with control dogs and CKCS with platelet count > 100,000/microl (both P < 0.0001). All platelet counts--except when made with the 2 counting chamber methods--were underestimated at platelet counts < or = 100,000/microl.
authors.affiliation: Department of Anatomy and Physiology, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
authors.forename: Lisbeth H
authors.initials: LH
authors.lastname: Olsen
authors.forename: Annemarie T
authors.initials: AT
authors.lastname: Kristensen
authors.forename: Karen
authors.initials: K
authors.lastname: Qvortrup
authors.forename: Henrik D
authors.initials: HD
authors.lastname: Pedersen
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000097:blood*; D004285:Dogs; D005260:Female; D008297:Male; D010976:Platelet Count / Q000379:methods / Q000662:veterinary*; D018709:Statistics, Nonparametric; D013921:Thrombocytopenia / Q000097:blood / Q000662:veterinary*