title: PCR-based identification of serotype 2 isolates of Actinobacillus pleuropneumoniae biovars I and II.
abstract: A genetic typing method utilizing PCR for the identification of Actinobacillus pleuropneumoniae serotype 2 isolates has been developed based on the in vitro amplification of a 1.4 kb DNA segment of the serotype 2 capsular polysaccharide genes cps2AB. The assay was tested with all serotype reference strains and a collection of 92 different A. pleuropneumoniae strains of all 15 serotypes of both biovars I and II, originating from 18 different countries worldwide. The cps2 based PCR identified the serotype 2 reference strain and all 12 serotype 2 collection strains contained in this set. DNA was not amplified from the remaining A. pleuropneumoniae reference and collection strains, indicating the PCR assay was highly specific. Furthermore, the PCR method detected all 31 A. pleuropneumoniae serotype 2 field isolates from diseased pigs that were identified in parallel as serotype 2 by agar gel diffusion. The serotype 2 PCR assay proved to be highly specific and reliable for the identification of serotype 2 isolates of A. pleuropneumoniae.
authors.affiliation: Institute of Veterinary Bacteriology, University of Berne, Laenggass-Strasse 122, CH-3001 Bern, Switzerland.
authors.forename: Daniela
authors.initials: D
authors.lastname: Hüssy
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Schlatter
authors.forename: Raymond
authors.initials: R
authors.lastname: Miserez
authors.forename: Thomas
authors.initials: T
authors.lastname: Inzana
authors.forename: Joachim
authors.initials: J
authors.lastname: Frey
mesh_terms: D000189:Actinobacillus Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D016667:Bacterial Capsules / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D011001:Pleuropneumonia / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial