title: The murine inhibitory receptor mSiglec-E is expressed broadly on cells of the innate immune system whereas mSiglec-F is restricted to eosinophils.
abstract: Murine (m) Siglec-E and mSiglec-F are recently discovered murine sialic acid-binding Ig-like lectins with tyrosine-based inhibitory signaling motifs. They are postulated to be the orthologs of human (h) siglec-7, -8 or -9 and siglec-5, respectively. We report here the first detailed characterization of mSiglec-E, and compare its expression pattern with mSiglec-F. Similar to hSiglec-7, mSiglec-E preferred alpha 2-8-linked disialic acid over alpha 2-3- and alpha 2-6-linked sialic acids. Using a specific Ab, FACS analysis demonstrated that mSiglec-E was expressed mainly on neutrophils in blood and their immature precursors in bone marrow. mSiglec-E was present on peritoneal cavity macrophages and on subsets of mature NK cells and splenic dendritic cells. mSiglec-E was also found ona novel population of peritoneal cavity B-1a-like cells and a subset of splenic B cells enriched in transitional T2 and marginal zone B cells. In striking contrast to mSiglec-E, mSiglec-F was expressed predominantly on eosinophils in blood and their precursors in the bone marrow. The distinct and largely non-overlapping expression profiles of mSiglec-E and mSiglec-F suggest that they play non-redundant roles in the innate immune system. mSiglec-E is likely to modulate the functions of several types of effector cells, whereas mSiglec-F is likely to be more restricted to eosinophil biology.
authors.affiliation: Division of Cell Biology and Immunology, The Wellcome Trust Biocentre at Dundee, University of Dundee, Dundee, GB.
authors.forename: Jiquan Q
authors.initials: JQ
authors.lastname: Zhang
authors.forename: Björn
authors.initials: B
authors.lastname: Biedermann
authors.forename: Lars
authors.initials: L
authors.lastname: Nitschke
authors.forename: Paul R
authors.initials: PR
authors.lastname: Crocker
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology* / Q000378:metabolism; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology* / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology* / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D004804:Eosinophils / Q000276:immunology* / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007113:Immunity, Innate; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology; D012689:Sequence Homology, Nucleic Acid; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013234:Stem Cells / Q000276:immunology / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D015214:Antigens, Differentiation, Myelomonocytic; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; C422908:Siglece protein, mouse; C443130:Siglecf protein, mouse