title: On the use of post-transcriptional processing elements in transgenes.
abstract: RNA processing events modulate final productivity of a given transgene. We have evaluated a series of RNA elements for their ability to enhance alpha1-antitrypsin production in mammary cells. Our results indicate the need for a case-by-case assessment of each construct design and the occurrence of gene silencing events in vivo.
authors.affiliation: Department of Gene Expression and Development, Roslin Institute (Edinburgh), Roslin, Midlothian, EH25 9PS, UK. bruce.whitelaw@bbsrc.ac.uk
authors.forename: C B A
authors.initials: CB
authors.lastname: Whitelaw
authors.forename: L
authors.initials: L
authors.lastname: Hiripi
authors.forename: E
authors.initials: E
authors.lastname: Farini
authors.forename: M L
authors.initials: ML
authors.lastname: Opsahl
authors.forename: Z
authors.initials: Z
authors.lastname: Bosze
mesh_terms: D000818:Animals; D020868:Gene Silencing; D018014:Gene Transfer Techniques; D051379:Mice; D008822:Mice, Transgenic; D012313:RNA / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D014162:Transfection; D019076:Transgenes
chemical_list: D012313:RNA