title: The binding of VIP36 and alpha-amylase in the secretory vesicles via high-mannose type glycans.
abstract: Vesicular integral protein of 36 kDa (VIP36) is an intracellular lectin recognizing high-mannose type glycans and is highly expressed in salivary glands, especially the parotid gland, which secretes alpha-amylase in large quantities. Here immunoelectron microscopy demonstrated that VIP36 was primarily localized to secretory vesicles in the glandula parotis of the rat, where alpha-amylase also resided. A secretory vesicle fraction, prepared by Percoll density gradient centrifugation, contained both VIP36 and alpha-amylase. Moreover, alpha-amylase that was localized to these secretory vesicles contained high-mannose type glycans. In addition, VIP36 coprecipitated with alpha-amylase in an endo H treatment-sensitive manner. These results suggest that VIP36 is involved in the secretion of alpha-amylase in the rat parotid gland.
authors.affiliation: Department of Biochemistry, Sasaki Institute, 2-2 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
authors.forename: Sayuri
authors.initials: S
authors.lastname: Hara-Kuge
authors.forename: Akira
authors.initials: A
authors.lastname: Seko
authors.forename: Osamu
authors.initials: O
authors.lastname: Shimada
authors.forename: Hisami
authors.initials: H
authors.lastname: Tosaka-Shimada
authors.forename: Katsuko
authors.initials: K
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D008297:Male; D037241:Mannose-Binding Lectins / Q000378:metabolism*; D016253:Microscopy, Immunoelectron; D010306:Parotid Gland / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D022142:Secretory Vesicles / Q000378:metabolism*; D000516:alpha-Amylases / Q000378:metabolism*
chemical_list: D037241:Mannose-Binding Lectins; D000516:alpha-Amylases