title: Genome-scan for loci involved in cleft lip with or without cleft palate in consanguineous families from Turkey.
abstract: Cleft lip with or without cleft palate (CL/P) is a common congenital anomaly, with birth prevalence ranging from 1/500 to 1/1,000. A number of genetic loci have shown positive linkage or association results in European Caucasian populations. The purpose of the current study was to assess whether any of those loci have positive results in Turkish Caucasian CL/P families, and to perform a 10 cM genome scan to identify other regions potentially containing cleft susceptibility loci. Eighteen affected individuals with consanguineous parents were identified as part of our on-going studies of orofacial clefts in Ankara, Turkey. Genotyped were 383 genome-scan markers, and 70 additional markers, including markers in six candidate loci or regions on chromosomes 2, 4, 6, 14, 17, and 19 (TGFA, D4S175, F13A1, TGFB3, D17S250, and APOC2) that have been implicated in other studies of families with orofacial clefting. LOD scores (two point and multiple point) and family-based association statistics (TDT) were calculated between each of the markers and CL/P. For the LOD score calculations, an autosomal recessive model was assumed for the inheritance of CL/P. Of the six candidate markers, significant TDT results were obtained with TGFA (P = 0.05). The most statistically significant multipoint results from the linkage genome scan were between putative genes controlling risk of CL/P and regions on chromosomes 4, 10, 12, and 15 (maximum multipoint HLOD's of 1.25, 1.30, 2.73, and 1.28 respectively). These results demonstrate the power of small numbers of families with inbred probands to detect linkage and association.
authors.affiliation: Center for Craniofacial and Dental Genetics, Division of Oral Biology, School of Dental Medicine, University of Pittsburgh, Suite 500 Cellomics Building, Pittsburgh, 100 Technology Drive, Pittsburgh, PA 15219, USA. marazita@sdmgenetics.pitt.edu
authors.forename: Mary L
authors.initials: ML
authors.lastname: Marazita
authors.forename: L Leigh
authors.initials: LL
authors.lastname: Field
authors.forename: Gökhan
authors.initials: G
authors.lastname: Tunçbilek
authors.forename: Margaret E
authors.initials: ME
authors.lastname: Cooper
authors.forename: Toby
authors.initials: T
authors.lastname: Goldstein
authors.forename: K Gürler
authors.initials: KG
authors.lastname: Gürsu
mesh_terms: D002879:Chromosomes, Human, Pair 10; D002881:Chromosomes, Human, Pair 12; D002884:Chromosomes, Human, Pair 15; D002894:Chromosomes, Human, Pair 4; D002971:Cleft Lip / Q000235:genetics*; D002972:Cleft Palate / Q000235:genetics*; D003241:Consanguinity; D005190:Family; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D015894:Genome, Human; D006801:Humans; D015810:Linkage Disequilibrium; D008126:Lod Score; D008297:Male; D010375:Pedigree; D014421:Turkey
chemical_list: D005819:Genetic Markers