title: Cytochrome P-450 family 1 in rat embryo cell culture immortalized by Rausher leukemia virus.
abstract: We studied comparative expression and activity of cytochrome P450 family 1 (CYP1) isoforms in rat embryo cells, both primary and immortalized by Rausher leukemia virus (RLV). In RLV-infected embryonal cells compared with the initial ones the expression levels of CYP1A1 and 1B1 mRNAs and benzo[a]pyrene (BP) hydroxylase activity were higher, regardless of their treatment with the CYP1 inducer 2,3,7,8-tetrachlorodibenzo-p-dioxin. The sensitivity to BP and 7,12-dimethylbenzo[a]anthracene was higher in the cells immortalized with RLV. The expression level of mRNAs of induction-mediating proteins aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator was the same in both cell cultures tested. Higher sensitivity of cells immortalized with RLV compared with the initial embryo cells to transforming effect of BP, which was described previously, is possibly associated with elevated expression of CYP1 isoforms.
authors.affiliation: Institute of Carcinogenesis, Cancer Research Center, Russian Academy of Medical Sciences, Moscow 115478, Russia. vakob@crc.umos.ru
authors.forename: A V
authors.initials: AV
authors.lastname: Vaiman
authors.forename: N P
authors.initials: NP
authors.lastname: Shcherbak
authors.forename: V A
authors.initials: VA
authors.lastname: Kobliakov
mesh_terms: D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000096:biosynthesis* / Q000235:genetics; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator; D002461:Cell Line, Transformed / Q000201:enzymology* / Q000821:virology; D002472:Cell Transformation, Viral / Q000187:drug effects; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis* / Q000235:genetics; D065633:Cytochrome P-450 CYP1B1; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D004622:Embryo, Mammalian / Q000166:cytology / Q000201:enzymology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011916:Rats, Inbred F344; D011925:Rauscher Virus; D018336:Receptors, Aryl Hydrocarbon / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013723:Teratogens / Q000494:pharmacology; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics
chemical_list: C497398:ARNT protein, rat; D004268:DNA-Binding Proteins; D007527:Isoenzymes; D000072317:Polychlorinated Dibenzodioxins; D012333:RNA, Messenger; D018336:Receptors, Aryl Hydrocarbon; D013723:Teratogens; D014157:Transcription Factors; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator; D001189:Aryl Hydrocarbon Hydroxylases; C585499:Cyp1b1 protein, rat; D019363:Cytochrome P-450 CYP1A1; D065633:Cytochrome P-450 CYP1B1