[The role of tissue factor expression in the invasive and metastatic ability of colorectal carcinoma].
OBJECTIVE
To investigate the role of tissue factor (TF) expression in the invasive and metastatic ability of colorectal carcinoma and explore the influence of TF on the invasive ability of HT-29 cells.


METHODS
TF expression of specimens from 85 colorectal carcinomas and 6 colorectal adenomas was observed by immunohistochemistry. The role of TF expression in prognosis and tumor invasion and metastasis was analyzed. The plasmids pcDNA3.1/Zeo bearing either sense or antisense-TFcDNA were transfected into HT-29 cells by the way of Lipofectamine 2000. TF proteins in transfected and untransfected HT-29cells were detected by Western blot. In vitro Matrigel invasion assays were performed to show the invasive ability of those cells.


RESULTS
TF expression was positive in 40 (47.1%) of 85 colorectal carcinoma specimens, but negative in normal mucosa and adenoma specimens. TF expression showed significant correlation with tumor invasive depth (r = 0.895, P < 0.01). TF expression showed significant correlation with synchronous and metachronous hepatic metastasis (r = 0.974, P < 0.01 and r = 0.963, P < 0.01 respectively). TF expression was a significant risk factor for hepatic metastasis (P < 0.01) and prognosis (P < 0.01). TF expression in HT-29 cells with sense/antisense-TFcDNA transfection was more/less than that of the cells without transfection. The invasive ability of HT-29 cells with sense-TFcDNA transfection was increased in vitro compared with the untransfected cells, but HT-29 cells with antisense-TFcDNA transfection got the contrary change.


CONCLUSIONS
TF may take part in the invasive and metastatic process of primary colorectal carcinoma, and TF expression may be an indicator of hepatic metastasis and prognosis for colorectal carcinoma patients. TF expression may increase the invasive ability of HT-29 cell in vitro.
Wan
Yuan-lian
YL
Department of General Surgery, The First Hospital of Peking University, Beijing 100034, China.
Yao
Hong-wei
HW
Ye
Jing-ming
JM
Liu
Yu-cun
YC
Wu
Tao
T
Wang
Xin
X
Pan
Yi-sheng
YS
Wu
Nan
N
Ju
Xiao-ming
XM
Zhu
Jing
J
Huang
Yan-ting
YT
D015153:Blotting, Western; D002465:Cell Movement; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D019073:HT29 Cells; D006801:Humans; D007150:Immunohistochemistry; D016015:Logistic Models; D015999:Multivariate Analysis; D013925:Thromboplastin / Q000032:analysis* / Q000235:genetics
D013925:Thromboplastin