title: Distribution of HIV-1 in the genomes of AIDS patients.
abstract: The localization of HIV-1 proviruses in compositional DNA fractions from 27 AIDS patients during the chronic phase of the disease with depletion of CD4+ and different levels of viremia showed the following. (1) At low viremia, proviruses are predominantly localized in the GC-richest isochores, which are characterized by an open chromatin structure; this result mimics findings on HIV-1 integration in early infected cells in culture. (2) At higher viremia, an increased distribution of proviruses in GC-poor isochores (which match the GC poorness of HIV-1) was found; this suggests a selection of cells in which the 'isopycnic' localization leads to a higher expression of proviruses and, in turn, to higher viremia. (3) At the highest viremia, integrations in GC-rich isochores are often predominant again, but generally not at the same level as in (1); this may be the consequence of new integrations from the extremely abundant RNA copies.
authors.affiliation: Laboratoire de Génétique Moléculaire, Institut Jacques Monod, Paris, France.
authors.forename: L
authors.initials: L
authors.lastname: Tsyba
authors.forename: A V
authors.initials: AV
authors.lastname: Rynditch
authors.forename: E
authors.initials: E
authors.lastname: Boeri
authors.forename: K
authors.initials: K
authors.lastname: Jabbari
authors.forename: G
authors.initials: G
authors.lastname: Bernardi
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics*; D001482:Base Composition; D015704:CD4 Antigens; D002843:Chromatin / Q000235:genetics; D002877:Chromosomes, Human; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D020862:GC Rich Sequence; D015894:Genome, Human; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D032085:Isochores; D011533:Proviruses / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D014766:Viremia / Q000235:genetics*; D016662:Virus Integration
chemical_list: D015704:CD4 Antigens; D002843:Chromatin; D004279:DNA, Viral; D032085:Isochores; D012367:RNA, Viral