Phospholipids of Clostridium perfringens: a reexamination.
We have identified phosphatidylethanolamine as one of the major phospholipids of Clostridium perfringens by two dimensional thin layer chromatography of the intact lipids and of their deacylation products and by liquid chromatography followed by mass spectrometry of the intact neutral phospholipid fraction. The principal fatty acids of phosphatidylethanolamine are myristic acid (14:0), lauric acid (12:0), and palmitic acid (16:0) and the major molecular species are 14:0,14:0 (26.3%); 12:0,14:0 (19.0%); 14:0,16:0 (22.4%) and 16:0,16:0 (17.6%). A similar distribution of molecular species was found in the other major phospholipid, O-alanyl phosphatidylglycerol.
Johnston
Norah C
NC
Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6076, USA.
Baker
John K
JK
Goldfine
Howard
H
D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D003016:Clostridium perfringens / Q000737:chemistry*; D005227:Fatty Acids / Q000737:chemistry / Q000302:isolation & purification; D007850:Lauric Acids / Q000737:chemistry / Q000302:isolation & purification; D013058:Mass Spectrometry; D019814:Myristic Acid / Q000737:chemistry / Q000302:isolation & purification; D019308:Palmitic Acid / Q000737:chemistry / Q000302:isolation & purification; D010714:Phosphatidylethanolamines / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D010715:Phosphatidylglycerols / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D010743:Phospholipids / Q000032:analysis* / Q000737:chemistry*
D005227:Fatty Acids; D007850:Lauric Acids; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D010743:Phospholipids; D019814:Myristic Acid; C030358:lauric acid; D019308:Palmitic Acid; C483858:phosphatidylethanolamine