A large semi-synthetic single-chain Fv phage display library based on chicken immunoglobulin genes.
BACKGROUND
Antibody fragments selected from large combinatorial libraries have numerous applications in diagnosis and therapy. Most existing antibody repertoires are derived from human immunoglobulin genes. Genes from other species can, however, also be used. Because of the way in which gene conversion introduces diversity, the naïve antibody repertoire of the chicken can easily be accessed using only two sets of primers.


RESULTS
With in vitro diagnostic applications in mind, we have constructed a large library of recombinant filamentous bacteriophages displaying single chain antibody fragments derived from combinatorial pairings of chicken variable heavy and light chains. Synthetically randomised complementarity determining regions are included in some of the heavy chains. Single chain antibody fragments that recognise haptens, proteins and virus particles were selected from this repertoire. Affinities of three different antibody fragments were determined using surface plasmon resonance. Two were in the low nanomolar and one in the subnanomolar range. To illustrate the practical value of antibodies from the library, phage displayed single chain fragments were incorporated into ELISAs aimed at detecting African horsesickness and bluetongue virus particles. Virus antibodies were detected in a competitive ELISA.


CONCLUSIONS
The chicken-derived phage library described here is expected to be a versatile source of recombinant antibody fragments directed against a wide variety of antigens. It has the potential to provide monoclonal reagents with applications in research and diagnostics. For in vitro applications, naïve phage libraries based on avian donors may prove to be useful adjuncts to the selectable antibody repertoires that already exist.
van Wyngaardt
Wouter
W
Immunology Division, Onderstepoort Veterinary Institute, Onderstepoort, 0110, Republic of South Africa. wouter@moon.ovi.ac.za
Malatji
Teresiah
T
Mashau
Cordelia
C
Fehrsen
Jeanni
J
Jordaan
Frances
F
Miltiadou
Dubravka
D
du Plessis
Dion H
DH
D000355:African Horse Sickness / Q000175:diagnosis / Q000276:immunology / Q000821:virology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D000914:Antibodies, Viral / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D001819:Bluetongue / Q000175:diagnosis / Q000276:immunology / Q000821:virology; D001820:Bluetongue virus / Q000276:immunology / Q000302:isolation & purification; D002645:Chickens / Q000235:genetics* / Q000276:immunology*; D022801:Complementarity Determining Regions / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005803:Genes, Immunoglobulin / Q000235:genetics*; D007128:Immunoglobulin Fragments / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D019151:Peptide Library; D020349:Surface Plasmon Resonance
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D022801:Complementarity Determining Regions; D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D019151:Peptide Library