Synthesis of 2'-substituted MMI linked nucleosidic dimers: an optimization study in search of high affinity oligonucleotides for use in antisense constructs.
The synthesis of a series of methylene(methylimino) (MMI) linked oligodeoxyribonucleotide dimers modified at the 2'-position with fluoro and/or methoxy groups and their incorporation into different sequences has been accomplished. From these dimers, bis 2'-OMe MMI dimer was selected for further studies based on its synthetic accessibility and the increased thermodynamic stability conferred upon oligonucleotides incorporating this modification.
Peoc'h
Didier
D
Department of Medicinal Chemistry, Isis Pharmaceuticals, Inc., Carlsbad, California 92008, USA.
Swayze
Eric E
EE
Bhat
Balkrishen
B
Sanghvi
Yogesh S
YS
D016373:DNA, Antisense / Q000737:chemistry; D019281:Dimerization; D009705:Nucleosides / Q000138:chemical synthesis* / Q000737:chemistry; D016372:RNA, Antisense / Q000737:chemistry
D016373:DNA, Antisense; D009705:Nucleosides; D016372:RNA, Antisense