The synovial proteome: analysis of fibroblast-like synoviocytes.
The present studies were initiated to determine the protein expression patterns of fibroblast-like synovial (FLS) cells derived from the synovia of rheumatoid arthritis patients. The cellular proteins were separated by two-dimensional polyacrylamide gel electrophoresis and the in-gel digested proteins were analyzed by matrix-assisted laser desorption ionization mass spectrometry. A total of 368 spots were examined and 254 identifications were made. The studies identified a number of proteins that have been implicated in the normal or pathological FLS function (e.g. uridine diphosphoglucose dehydrogenase, galectin 1 and galectin 3) or that have been characterized as potential autoantigens in rheumatoid arthritis (e.g. BiP, colligin, HC gp-39). A novel uncharacterized protein product of chromosome 19 open reading frame 10 was also detected as an apparently major component of FLS cells. These results demonstrate the utility of high-content proteomic approaches in the analysis of FLS composition.
Dasuri
Kumar
K
Rheumatic Diseases Research Laboratory, University of Manitoba, Winnipeg, Canada.
Antonovici
Mihaela
M
Chen
Keding
K
Wong
Ken
K
Standing
Kenneth
K
Ens
Werner
W
El-Gabalawy
Hani
H
Wilkins
John A
JA
D002457:Cell Extracts / Q000737:chemistry; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005347:Fibroblasts / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D007719:Knee Joint; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013583:Synovial Membrane / Q000166:cytology*
D002457:Cell Extracts; D020543:Proteome