Studies on a novel carbon source and cosolvent for lipase production by Candida rugosa.
Oleic acid esters were shown to be the best carbon source for both cell growth and lipase production by Candida rugosa. Use of a cosolvent, dodecane, in fermentations improved the solubility of solid substrates and increased oxygen solubility. This resulted in the highest lipase activity in batch fermentation with glycerol trioleate and dodecane. Lipase activity reached 77.1 units ml(-1).
Wei
Dongzhi
D
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science & Technology, 200237 Shanghai, P.R. China. dzhwei@ecust.edu.cn
Zhang
Li-Ying
LY
Song
Qingxun
Q
D000473:Alkanes / Q000378:metabolism*; D002175:Candida / Q000201:enzymology*; D004795:Enzyme Stability; D008049:Lipase / Q000096:biosynthesis* / Q000302:isolation & purification; D009829:Oleic Acids / Q000378:metabolism; D012995:Solubility; D014304:Triolein / Q000378:metabolism*
D000473:Alkanes; D009829:Oleic Acids; C007548:n-dodecane; D014304:Triolein; D008049:Lipase