Anti-Gal IgG potentiates natural killer cell migration across porcine endothelium via endothelial cell activation and increased natural killer cell motility triggered by CD16 cross-linking.
Xenoreactive antibodies (Ab) are important for the development of acute vascular rejection (AVR) of xenografts characterized by monocytes, natural killer (NK) cells and neutrophils infiltrating the graft. The mechanisms by which anti-galactose alpha 1,3galactose (alpha-Gal) IgG influence NK cell migration across porcine aortic endothelium (PAEC) were investigated. NK cell migration across PAEC increased in the presence of anti-alpha-Gal IgG. Anti-alpha-Gal IgG exposure activated PAEC as shown by an increased expression of CD62E and CD106. NK cells adhered, spread and showed motile forms on plastic surfaces coated with human IgG, IgG Fc and on mAb against CD16, but not on mouse IgG or BSA, suggesting that CD16 cross-linking can mediate increased adhesiveness. Increased NK cell motility was observed on Boyden filters coated with human IgG, IgG Fc, and mAb against CD16 and the alpha 4, alpha 5, alpha L, beta 1 and beta 2 integrin chains. No motile response was seen on mouse IgGor CD7, CD56 and alpha 6 integrin mAb. NK cell migration on human IgG and anti-CD16 Ab was blocked by anti-CD16 or anti-beta 2, but not anti-beta 1 Ab, implying that the motile response triggered by CD16 cross-linking is mediated via beta 2 integrins. Preformed or induced anti-alpha-Gal IgG may therefore contribute to AVR by stimulating innate immune cell infiltration of the graft.
Hauzenberger
Elenor
E
Division of Clinical Immunology, Karolinska Institutet, Huddinge University Hospital AB, Stockholm, Sweden.
Klominek
Julius
J
Holgersson
Jan
J
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000910:Antibodies, Heterophile / Q000276:immunology; D018821:CD18 Antigens / Q000378:metabolism; D002448:Cell Adhesion / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D003432:Cross-Linking Reagents; D004187:Disaccharides / Q000276:immunology*; D019040:E-Selectin / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism* / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000276:immunology*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D017452:Receptors, IgG / Q000276:immunology / Q000378:metabolism*; D013552:Swine; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
D000888:Antibodies, Anti-Idiotypic; D000910:Antibodies, Heterophile; D018821:CD18 Antigens; D003432:Cross-Linking Reagents; D004187:Disaccharides; D019040:E-Selectin; D017452:Receptors, IgG; D019010:Vascular Cell Adhesion Molecule-1; C048844:anti-IgG; C055075:galactosyl-(1-3)galactose