CD36 overexpression in human brain correlates with beta-amyloid deposition but not with Alzheimer's disease.
Scavenger receptors recently have been related to Alzheimer's disease, although it is still unclear whether they contribute to the pathogenesis of the disease or reflect an inflammatory response to the deposition of amyloid beta-protein (Abeta). In this study we demonstrate that CD36, a class B scavenger receptor, is highly expressed in the cerebral cortex of Alzheimer's disease patients and cognitively normal aged subjects with diffuse amyloid plaques compared with age-matched amyloid-free control brains. Moreover, in vitro experiments indicated that Abeta is able to induce CD36 expression in neuronal cells after 24 h treatment. The interaction between CD36 and Abeta has been reported to trigger oxidant production by macrophages and microglia. In line with this observation, we found an increased presence of nitrated proteins in brains showing Abeta loads and CD36 overexpression, independent of the occurrence of Alzheimer's disease pathologic features.
Ricciarelli
Roberta
R
Department of Experimental Medicine, University of Genoa, 16132 Genoa, Italy. riciarelli@medicina.unige.it
D'Abramo
Cristina
C
Zingg
Jean-Marc
JM
Giliberto
Luca
L
Markesbery
William
W
Azzi
Angelo
A
Marinari
Umberto M
UM
Pronzato
Maria A
MA
Tabaton
Massimo
M
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000378:metabolism* / Q000502:physiology*; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D018955:CD36 Antigens / Q000096:biosynthesis* / Q000378:metabolism; D005609:Free Radicals; D005625:Frontal Lobe / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D015151:Immunoblotting; D008264:Macrophages / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D016877:Oxidants / Q000378:metabolism; D018384:Oxidative Stress; D010446:Peptide Fragments / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D011971:Receptors, Immunologic / Q000378:metabolism; D051116:Receptors, Scavenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051122:Scavenger Receptors, Class B; D013997:Time Factors
D016229:Amyloid beta-Peptides; D018955:CD36 Antigens; D005609:Free Radicals; D016877:Oxidants; D010446:Peptide Fragments; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D051116:Receptors, Scavenger; C495124:SCARB1 protein, human; D051122:Scavenger Receptors, Class B; C075222:amyloid beta-protein (1-42)