Effect of a paraneoplastic cerebellar degeneration-associated neural protein on B-myb promoter activity.
In this study, we have shown that a paraneoplastic cerebellar degeneration (PCD)-associated antigen, pcd17, binds to a cell cycle-related protein, MRG15. MRG15 derepresses the E2F-responsive B-myb promoter. The pcd17 antigen inhibits the derepression of the B-myb transcriptional activity by MRG15, and, as a result, pcd17 represses the promoter. Delivery of anti-Purkinje cell antibodies (anti-Yo) into the cells inhibits the repression of B-myb promoter activity by pcd17. Because derepression of the B-myb promoter has been implicated in neuronal death, the results suggest the possible role of the antibodies in the pathogenesis of PCD.
Sakai
Koichiro
K
Department of Neurology, Kanazawa Medical University, Kahoku-gun, 920-0293 Ishikawa, Japan. ksakai@kanazawa-med.ac.jp
Kitagawa
Yoko
Y
Saiki
Shinji
S
Saiki
Misuzu
M
Hirose
Genjiro
G
D000818:Animals; D019556:COS Cells; D018797:Cell Cycle Proteins; D002522:Chlorocebus aethiops; D002868:Chromosomal Proteins, Non-Histone / Q000187:drug effects / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D050684:E2F Transcription Factors; D007074:Immunoglobulin G / Q000494:pharmacology; D009363:Neoplasm Proteins / Q000276:immunology; D009410:Nerve Degeneration / Q000378:metabolism / Q000473:pathology*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D020362:Paraneoplastic Cerebellar Degeneration / Q000503:physiopathology*; D011401:Promoter Regions, Genetic; D011689:Purkinje Cells / Q000276:immunology* / Q000473:pathology; D015534:Trans-Activators / Q000187:drug effects* / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D007074:Immunoglobulin G; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D015534:Trans-Activators; D014157:Transcription Factors