Nested PCR assay for detection of Leishmania donovani in slit aspirates from post-kala-azar dermal Leishmaniasis Lesions.
A nested PCR assay to detect parasite DNA in slit aspirates from skin lesions of patients with post-kala-azar dermal lesihmaniasis (PKDL) is described. PCR results were positive in 27 of 29 (93%) samples by nested PCR assay, while only 20 of 29 (69%) were positive in a primary PCR assay. The nested PCR assay allowed reliable diagnosis of PKDL in a noninvasive manner.
Sreenivas
Gannavaram
G
Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi, India.
Ansari
N A
NA
Kataria
Joginder
J
Salotra
Poonam
P
D000818:Animals; D001707:Biopsy, Needle / Q000379:methods; D018105:DNA, Kinetoplast / Q000032:analysis; D006801:Humans; D007893:Leishmania donovani / Q000235:genetics / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology* / Q000473:pathology; D007898:Leishmaniasis, Visceral / Q000150:complications* / Q000469:parasitology / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012867:Skin / Q000469:parasitology / Q000473:pathology*
D018105:DNA, Kinetoplast