Molecular cloning of mouse amino acid transport system B0, a neutral amino acid transporter related to Hartnup disorder.
Resorption of amino acids in kidney and intestine is mediated by transporters, which prefer groups of amino acids with similar physico-chemical properties. It is generally assumed that most neutral amino acids are transported across the apical membrane of epithelial cells by system B(0). Here we have characterized a novel member of the Na(+)-dependent neurotransmitter transporter family (B(0)AT1) isolated from mouse kidney, which shows all properties of system B(0). Flux experiments showed that the transporter is Na(+)-dependent, electrogenic, and actively transports most neutral amino acids but not anionic or cationic amino acids. Superfusion of mB(0)AT1-expressing oocytes with neutral amino acids generated inward currents, which were proportional to the fluxes observed with labeled amino acids. In situ hybridization showed strong expression in intestinal microvilli and in the proximal tubule of the kidney. Expression of mouse B(0)AT1 was restricted to kidney, intestine, and skin. It is generally assumed that mutations of the system B(0) transporter underlie autosomal recessive Hartnup disorder. In support of this notion mB(0)AT1 is located on mouse chromosome 13 in a region syntenic to human chromosome 5p15, the locus of Hartnup disorder. Thus, the human homologue of this transporter is an excellent functional and positional candidate for Hartnup disorder.
Bröer
Angelika
A
School of Biochemistry and Molecular Biology, Australian National University, Canberra ACT 0200, Australia.
Klingel
Karin
K
Kowalczuk
Sonja
S
Rasko
John E J
JE
Cavanaugh
Juleen
J
Bröer
Stefan
S
D000595:Amino Acid Sequence; D026905:Amino Acid Transport Systems / Q000235:genetics*; D026921:Amino Acid Transport Systems, Neutral / Q000235:genetics*; D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D000838:Anions; D001483:Base Sequence; D001692:Biological Transport; D002412:Cations; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D004594:Electrophysiology; D006250:Hartnup Disease / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D017403:In Situ Hybridization; D007413:Intestinal Mucosa / Q000378:metabolism; D007477:Ions; D007668:Kidney / Q000378:metabolism; D007930:Leucine / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008954:Models, Biological; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D009865:Oocytes / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D010802:Phylogeny; D010957:Plasmids / Q000378:metabolism; D017434:Protein Structure, Tertiary; D018075:RNA, Complementary / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism; D013379:Substrate Specificity; D013997:Time Factors
D026905:Amino Acid Transport Systems; D026921:Amino Acid Transport Systems, Neutral; D000596:Amino Acids; D000838:Anions; D002412:Cations; D018076:DNA, Complementary; D007477:Ions; D010455:Peptides; D018075:RNA, Complementary; D012333:RNA, Messenger; D007930:Leucine