Leishmania donovani complex: genotyping with the ribosomal internal transcribed spacer and the mini-exon.
Intergenic region typing by restriction analysis of the ribosomal internal transcribed spacer (ITS) and mini-exon provide diagnostic markers for some Leishmania. Here, we evaluate restriction analysis of these targets for genotyping and phylogenetic analysis within the Leishmania donovani complex (agents of visceral leishmaniasis). Each method was useful for genotyping of both L. donovani complex strains and Old World Leishmania species. The targets produced less robust groups than gp63 intergenic regions, but support the need for re-evaluation of the taxonomy of the L. donovani complex.
Mauricio
I L
IL
Pathogen Molecular Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK. isabel.mauricio@lshtm.ac.uk
Stothard
J R
JR
Miles
M A
MA
D000818:Animals; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics*; D005091:Exons / Q000235:genetics; D007893:Leishmania donovani / Q000145:classification / Q000235:genetics*; D007898:Leishmaniasis, Visceral / Q000469:parasitology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D016054:DNA, Protozoan; D021903:DNA, Ribosomal Spacer