Genetic structure of seventy cocoyam (Xanthosoma sagittifolium, Linn, Schott) accessions in Ghana based on RAPD.
Seventy cocoyam accessions collected from the eastern and Volta regions of Ghana were studied using RAPD technique. Ten primers were used to study the genetic diversity and structure of the experimental material and a total of 120 different bands were detected. Levels of polymorphic fragments detected by the ten primers ranged from 69.2% to 100%. In the accessions from the eastern region, the mean observed and effective number of alleles per individual per population and mean Nei's gene diversity were 1.99+/-0.01, 1.53+/-0.12 and 0.312+/-0.05, respectively. Mean observed and effective number of alleles per individual per population and Nei's gene diversity were 1.76+/-0.17, 1.44+/-0.16 and 0.264+/-0.08, respectively for the accessions from the Volta region. Considering the entire collections, the mean observed and effective number of alleles per individual per population and Nei's gene diversity for primers were 1.99+/-0.02, 1.54+/-0.12 and 0.319+/-0.05, respectively. In terms of Nei's F-statistics in the subdivided populations for all primers, the overall gene diversity (Ht) ranged from 0.230 to 0.396 with a mean of 0.313+0.06, within sample gene diversity (Hs) ranged from 0.214 to 0.372 with a mean of 0.286+0.06, gene differentiation (Gst) ranged between 0.005 and 0.178 with a mean of 0.086. Geneflow estimate ranged between 2.309 and 99.500 with a mean of 5.314. The accessions grouped into three main clusters. Accession BD96/183 was the most diverse and may be incorporated into cocoyam breeding programs. The 70 accessions did not cluster into their distinct geographical regions suggesting that there may have been movement of germplasm across the two regions.
Offei
S K
SK
Molecular Biology Laboratory, Department of Crop Science, University of Ghana, Legon, Ghana.
Asante
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IK
Danquah
E Y
EY
D017931:DNA Primers; D018744:DNA, Plant / Q000032:analysis*; D005787:Gene Frequency; D017343:Genes, Plant / Q000235:genetics*; D005819:Genetic Markers; D014644:Genetic Variation / Q000235:genetics*; D005828:Genetics, Population; D005869:Ghana; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D031063:Xanthosoma / Q000235:genetics*
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers