Linear phase imaging using differential interference contrast microscopy.
We propose an extension to Nomarski differential interference contrast microscopy that enables isotropic linear phase imaging. The method combines phase shifting, two directions of shear and Fourier-space integration using a modified spiral phase transform. We simulated the method using a phantom object with spatially varying amplitude and phase. Simulated results show good agreement between the final phase image and the object phase, and demonstrate resistance to imaging noise.
Arnison
M R
MR
Physical Optics Laboratory, School of Physics, University of Sydney, NSW 2006, Australia. mra@cisra.canon.com.au
Larkin
K G
KG
Sheppard
C J R
CJ
Smith
N I
NI
Cogswell
C J
CJ
D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D008962:Models, Theoretical