Cord blood (CB) stem cells for wound repair. Preliminary report of 2 cases.
In 2 patients, to promote skin wound/lesion repair we used fibrin-platelet glue combined with HLA compatible (2 mismatches accepted) buffy coats containing CD 34+ cord blood cells. The fibrin platelet glue was prepared with autologous apheresis platelets and cryoprecipitate. The original product was divided into 3 and 4 aliquots respectively for a correspondent number of applications. At each application, the margins of the lesion were infiltrated with 3 ml of cord blood buffy coat, containing 30 x 10(3) CD 34+ cells. No graft versus tissue reaction was seen in our patients in a follow-up of 3-7 months. The level of improvement, scored arbitrarily from 0 to 4, was 3 and 4, respectively. Our conclusion is that the use of cord blood cells along with fibrin platelet glue is of clinical interest.
Valbonesi
M
M
Immunohematology Services, Department of Immunohematology, San Martino University Hospital, Largo Rosanna Benzi 10, 16132 Genoa, Italy. maurovalbonesi@smartino.ge.it
Giannini
G
G
Migliori
F
F
Dalla Costa
R
R
Dejana
A M
AM
D000368:Aged; D000369:Aged, 80 and over; D018952:Antigens, CD34 / Q000096:biosynthesis; D001781:Blood Component Removal; D001792:Blood Platelets / Q000378:metabolism; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D015718:Fibrin Tissue Adhesive / Q000737:chemistry; D006680:HLA Antigens / Q000737:chemistry; D006650:Histocompatibility Testing; D006801:Humans; D008297:Male; D013234:Stem Cells / Q000166:cytology*; D013997:Time Factors; D014014:Tissue Adhesives / Q000737:chemistry; D014945:Wound Healing
D018952:Antigens, CD34; D015718:Fibrin Tissue Adhesive; D006680:HLA Antigens; D014014:Tissue Adhesives