[Mitochondria transfer from self-granular cells to improve embryos' quality].
OBJECTIVE
To improve embryos' quality and pregnancy rate with the method of self-mitochondria transfer.


METHODS
All the 18 cases of women with repeated in vitro fertilization-embryo transfer (IVF-ET)-treatment failure or older than 37 years were treated with the long gonadotropin releasing hormone-agonist (GnRH-a) regimens. The oocytes were divided into two groups: intracytoplasmic sperm injection (ICSI) group and mitochondria transfer group if the number of the individual patients' oocytes was more than four. In mitochondria transfer group, mitochondria were prepared by different centrifugation after the granulosa cells were trimmed from the oocytes or collected from follicular fluid and homogenated. Mitochondria and immobilized sperm were injected into oocytes by micromanipulation. The fertilization rate and embryos' quality were compared.


RESULTS
The fertilization rate was 74.4% in mitochondria transfer group, and 76.8% in ICSI group, with no statistical difference (P > 0.05). The good quality embryo rate in mitochondria transfer group was 59.4%, and it was 34.9% in ICSI group. The difference was significant (P < 0.05). There were 7 clinical pregnancies in the 18 cases.


CONCLUSIONS
Self-mitochondria transfer can improve embryos' quality and pregnancy rate without influence on fertilization rate.
Kong
Ling-hong
LH
Department of Reproductive Medicine Center, First Affiliated Hospital, First Military Medical University, Guangzhou 510515, China.
Liu
Zhong
Z
Li
Hong
H
Zhu
Liang
L
Chen
Si-mei
SM
Chen
Shi-ling
SL
Xing
Fu-qi
FQ
D000328:Adult; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D008928:Mitochondria / Q000637:transplantation*; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods; D016896:Treatment Outcome