title: Determination of derivatized l-alanosine in plasma by liquid chromatography-tandem mass spectrometry.
abstract: A sensitive method was developed for quantitation of the cytotoxic antibiotic l-alanosine in human plasma. Alanosine was extracted from plasma by anion-exchange solid phase extraction, derivatized with dansyl chloride and analyzed by liquid chromatography-tandem mass spectrometry using atmospheric pressure chemical ionization in negative mode. Dansylation led to 50-fold improvement of method sensitivity over non-dansylated alanosine with a resulting 20 ng/ml limit of alanosine quantitation in plasma being achieved. The method was validated and applied for clinical studies of alanosine administered to cancer patients.
authors.affiliation: Maxxam Analytics Inc., 5540 McAdam Road, Mississauga, Ont., Canada L4Z 1P1. alex.gantverg@maxxamanalytics.com
authors.forename: Alex
authors.initials: A
authors.lastname: Gantverg
authors.forename: Gary
authors.initials: G
authors.lastname: Elliott
authors.forename: Jean
authors.initials: J
authors.lastname: Pineault
authors.forename: Roger
authors.initials: R
authors.lastname: Demers
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives / Q000097:blood*; D000903:Antibiotics, Antineoplastic / Q000097:blood*; D000970:Antineoplastic Agents / Q000097:blood*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D000903:Antibiotics, Antineoplastic; D000970:Antineoplastic Agents; C000082:alanosine; D000409:Alanine