Multiple trypsin inhibitors from Momordica cochinchinensis seeds, the Chinese drug mubiezhi.
Five trypsin inhibitors, with N-terminal sequences demonstrating homology to each other and exhibiting a molecular weight of 5100, 4800, 4400, 4100, and 3900, respectively, were isolated from Momordica cochinchinensis seeds with a protocol involving acid extraction, ion exchange chromatography on SP-Sepharose chromatography, and RP-HPLC on a C18 column. Specific inhibitory activity against trypsin was demonstrated by the trypsin isoinhibitors with Ki values ranging from 5.3 x 10(-8) to 1.8 x 10(-6) M. None of the isoinhibitors could be cleaved by trypsin.
Wong
Ricardo C H
RC
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
Fong
W P
WP
Ng
T B
TB
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D029775:Momordica / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D014361:Trypsin Inhibitors / Q000737:chemistry*
D010940:Plant Proteins; D014361:Trypsin Inhibitors