title: Coronamycins, peptide antibiotics produced by a verticillate Streptomyces sp. (MSU-2110) endophytic on Monstera sp.
abstract: Coronamycin is a complex of novel peptide antibiotics with activity against pythiaceous fungi and the human fungal pathogen Cryptococcus neoformans. It is also active against the malarial parasite, Plasmodium falciparum, with an IC(50) of 9.0 ng ml(-1). Coronamycin is produced by a verticillate Streptomyces sp. isolated as an endophyte from an epiphytic vine, Monstera sp., found in the Manu region of the upper Amazon of Peru. Bioassay-guided fractionation of the fermentation broths of this endophyte on silica gel and HPLC chromatography yielded two principal, inseparable, peptides with masses of 1217.9 and 1203.8 Da. Three other minor, but related components, are also present in the preparation. Amino acid analysis of coronamycin revealed residues of component 1, component 2, methionine, tyrosine and leucine at a ratio of 2:2:1:1:3. Other compounds with antifungal activities are also produced by this endophytic streptomycete.
authors.affiliation: Department of Plant Sciences, Montana State University, Bozeman, MT 59717, USA.
authors.forename: David
authors.initials: D
authors.lastname: Ezra
authors.affiliation: Department of Plant Sciences, Montana State University, Bozeman, MT 59717, USA.
authors.forename: Uvidelio F
authors.initials: UF
authors.lastname: Castillo
authors.affiliation: Department of Plant Sciences, Montana State University, Bozeman, MT 59717, USA.
authors.forename: Gary A
authors.initials: GA
authors.lastname: Strobel
authors.affiliation: Department of Integrated Biology, Brigham Young University, Provo, UT 84602, USA.
authors.forename: Wilford M
authors.initials: WM
authors.lastname: Hess
authors.affiliation: Department of Microbiology, Brigham Young University, Provo, UT 84602, USA.
authors.forename: Heidi
authors.initials: H
authors.lastname: Porter
authors.affiliation: Department of Microbiology, Brigham Young University, Provo, UT 84602, USA.
authors.forename: James B
authors.initials: JB
authors.lastname: Jensen
authors.affiliation: Department of Neurology, Harvard Medical School, and Center for Neurologic Diseases, Brigham and Women's Hospital, Boston, MA 02115, USA.
authors.forename: Margaret A M
authors.initials: MAM
authors.lastname: Condron
authors.affiliation: Department of Neurology, Harvard Medical School, and Center for Neurologic Diseases, Brigham and Women's Hospital, Boston, MA 02115, USA.
authors.forename: David B
authors.initials: DB
authors.lastname: Teplow
authors.affiliation: Department of Chemistry, Montana State University, Bozeman, MT 59717, USA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Sears
authors.affiliation: Novozymes Biotech Inc., 1445 Drew Ave, Davis, CA 95616, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Maranta
authors.affiliation: Novozymes Biotech Inc., 1445 Drew Ave, Davis, CA 95616, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Hunter
authors.affiliation: Novozymes Biotech Inc., 1445 Drew Ave, Davis, CA 95616, USA.
authors.forename: Barbara
authors.initials: B
authors.lastname: Weber
authors.affiliation: Novozymes Biotech Inc., 1445 Drew Ave, Davis, CA 95616, USA.
authors.forename: Debbie
authors.initials: D
authors.lastname: Yaver
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D029064:Araceae / Q000382:microbiology* / Q000648:ultrastructure; D001419:Bacteria / Q000187:drug effects; D002460:Cell Line; D005658:Fungi / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D021261:Parasitic Sensitivity Tests; D010455:Peptides; D010963:Plasmodium falciparum / Q000187:drug effects; D017422:Sequence Analysis, DNA; D013172:Spores, Fungal / Q000648:ultrastructure; D013302:Streptomyces / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D000900:Anti-Bacterial Agents; D010455:Peptides