title: Hypermethylation of the PTEN gene in ovarian cancer cell lines.
abstract: This study was performed to investigate the hypermethylation status of the PTEN gene in ovarian cancer. To this end, we incubated eight ovarian cancer cell lines with the demethylating agent 5-aza-2' deoxycytidine in three different concentrations for 5 days. Subsequently, the PTEN expression was quantified by both real time RT-PCR and quantitative western analyses. PTEN mRNA varied considerably in response to demethylation whereas PTEN protein concentrations remained constant in all cell lines except OAW42 cells (12.5%). The data suggest that PTEN is highly regulated at translational level. However, methylation of the PTEN gene plays a subordinate role in ovarian cancer.
authors.affiliation: Department of Natural Sciences, University of Applied Sciences, Augustin, Germany. thomas.schoendorf@fh-bonn-rhein-sieg.de
authors.forename: Thomas
authors.initials: T
authors.lastname: Schöndorf
authors.forename: Matthias P
authors.initials: MP
authors.lastname: Ebert
authors.forename: Juliane
authors.initials: J
authors.lastname: Hoffmann
authors.forename: Martina
authors.initials: M
authors.lastname: Becker
authors.forename: Natasha
authors.initials: N
authors.lastname: Moser
authors.forename: Saziye
authors.initials: S
authors.lastname: Pur
authors.forename: Uwe-Jochen
authors.initials: UJ
authors.lastname: Göhring
authors.forename: Maria-Paz
authors.initials: MP
authors.lastname: Weisshaar
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D001374:Azacitidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000037:antagonists & inhibitors; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D000077209:Decitabine; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D000964:Antimetabolites, Antineoplastic; D004273:DNA, Neoplasm; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins; D000077209:Decitabine; D015254:DNA Modification Methylases; D010744:Phosphoric Monoester Hydrolases; D001374:Azacitidine