Development of venom toxin-specific antibodies by DNA immunisation: rationale and strategies to improve therapy of viper envenoming.
DNA vaccination induces potent cellular immune responses against infectious and parasitic intracellular pathogens. This paper illustrates that DNA immunisation protocols can be adapted to induce high titre antibody responses with potential to improve the treatment of systemic snake envenoming that kills 20000 people annually in Africa. Envenoming by the saw-scaled vipers and puff adders are responsible for the majority of these deaths. DNA sequences encoding haemorrhagic, pro- and anti-coagulant and other haemostasis-disruptive venom toxins from these vipers showed extensive cross-specific and cross-generic sequence and structural similarities. The predicted antigenic profiles of these toxin sequences are utilised to design DNA immunisation constructs to generate toxin-specific antibodies with potential to polyspecifically neutralise venoms from the most medically-important African vipers.
Harrison
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Alistair Reid Venom Research Unit, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK. robharr@liverpool.ac.uk
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D000997:Antivenins / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids / Q000276:immunology; D012909:Snake Bites / Q000628:therapy*; D019444:Vaccines, DNA / Q000276:immunology; D014757:Viper Venoms / Q000276:immunology*
D000997:Antivenins; D019444:Vaccines, DNA; D014757:Viper Venoms