title: DNA replication in thermophiles.
abstract: DNA replication enzymes in the thermophilic Archaea have previously attracted attention due to their obvious use in methods such as PCR. The proofreading ability of the Pyrococcus furiosus DNA polymerase has resulted in a commercially successful product (Pfu polymerase). One of the many notable features of the Archaea is the fact that their DNA processing enzymes appear on the whole to be more like those found in eukaryotes than bacteria. These proteins also appear to be simpler versions of those found in eukaryotes. For these reasons, archaeal organisms make potentially interesting model systems to explore the molecular mechanisms of processes such as DNA replication, repair and recombination. Why archaeal DNA-manipulation systems were adopted over bacterial systems by eukaryotic cells remains a most interesting question that we suggest may be linked to thermophily.
authors.affiliation: Centre for Extremophile Research, Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, U.K.
authors.forename: A I
authors.initials: AI
authors.lastname: Majerník
authors.forename: E R
authors.initials: ER
authors.lastname: Jenkinson
authors.forename: J P J
authors.initials: JP
authors.lastname: Chong
mesh_terms: D001105:Archaea / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004265:DNA Helicases / Q000235:genetics; D004260:DNA Repair; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D053488:DnaB Helicases; D004926:Escherichia coli / Q000235:genetics; D019766:Genes, Archaeal; D020745:Genome, Archaeal; D008957:Models, Genetic; D020027:Pyrococcus furiosus / Q000201:enzymology*; D011995:Recombination, Genetic
chemical_list: D001426:Bacterial Proteins; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D004265:DNA Helicases; D053488:DnaB Helicases