[A plate method to screen for microorganisms producing xylose isomerase].
A plate method was developed to screen for xylose isomerase-producing microorganisms based on the use of 2,3,5-triphenyltetrazolium as an indicator of D-xylulose, the D-xylose isomerization product. The use of this method allows microorganisms to be differentiated by the character of the enzyme synthesis (inducible or constitutive).
Sapunova
L I
LI
Institute of Microbiology, National Academy of Sciences of Belarus, Minsk, 220141 Belarus. enzyme@mbio.bas-net.by
Lobanok
A G
AG
Kazakevich
I O
IO
Evtushenkov
A N
AN
D019747:Aldose-Ketose Isomerases / Q000032:analysis* / Q000096:biosynthesis; D001173:Arthrobacter / Q000302:isolation & purification* / Q000378:metabolism; D015169:Colony Count, Microbial / Q000379:methods; D004396:Coloring Agents; D013778:Tetrazolium Salts
D004396:Coloring Agents; D013778:Tetrazolium Salts; C009591:triphenyltetrazolium; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase