title: Purification of an ACE inhibitory peptide after hydrolysis of sunflower (Helianthus annuus L.) protein isolates.
abstract: Sunflower protein isolates and the proteases pepsin and pancreatin were used for the production of protein hydrolysates that inhibit angiotensin-I converting enzyme (ACE). Hydrolysates obtained after 3 h of incubation with pepsin and 3 h with pancreatin were studied. An ACE inhibitory peptide with the sequence Phe-Val-Asn-Pro-Gln-Ala-Gly-Ser was obtained by G-50 gel filtration chromatography and high-performance liquid chromatography C18 reverse phase chromatography. This peptide corresponds to a fragment of helianthinin, the 11S globulin from sunflower seeds, which is the main storage protein in sunflower. These results show that sunflower seed proteins are a potential source of ACE inhibitory peptides when hydrolyzed with pepsin and pancreatin.
authors.affiliation: Instituto de la Grasa, Padre García Tejero 4, 41012-Sevilla, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Megías
authors.forename: Maria
authors.initials: M
authors.lastname: del Mar Yust
authors.forename: Justo
authors.initials: J
authors.lastname: Pedroche
authors.forename: Hassan
authors.initials: H
authors.lastname: Lquari
authors.forename: Julio
authors.initials: J
authors.lastname: Girón-Calle
authors.forename: Manuel
authors.initials: M
authors.lastname: Alaiz
authors.forename: Francisco
authors.initials: F
authors.lastname: Millán
authors.forename: Javier
authors.initials: J
authors.lastname: Vioque
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000302:isolation & purification*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006368:Helianthus / Q000737:chemistry*; D006868:Hydrolysis; D010194:Pancreatin / Q000378:metabolism; D010434:Pepsin A / Q000378:metabolism; D010455:Peptides / Q000302:isolation & purification* / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D010455:Peptides; D010940:Plant Proteins; D010194:Pancreatin; D010434:Pepsin A