Adipose angiotensinogen secretion, blood pressure, and AGT M235T polymorphism in obese patients.
OBJECTIVE
To investigate AGT secretion in cultured adipocytes from obese patients and its relationship with obesity-related phenotypes, blood pressure, and the M235T polymorphism in the AGT gene.


METHODS
Measurements, including anthropometry, body composition (DXA), and blood pressure, were performed in 61 overweight or obese women (BMI: 28 to 68 kg/m(2)). A subcutaneous abdominal adipose tissue biopsy was used for adipocyte size determination and quantification of AGT secretion in the medium of cultured adipocytes. AGT M235T genotype was determined using polymerase chain reaction-restriction fragment length polymorphism.


RESULTS
Adipose secretion of the AGT protein (range, 140 to 2575 ng/10(6) cells/24 h) was not significantly correlated with BMI, body fat, or blood pressure and did not vary according to the M235T polymorphism in the AGT gene. However, the AGT M235T polymorphism was associated with adipocyte size (111.6 +/- 2.8, 108.8 +/- 1.9, 118.2 +/- 2.6 micro m in MM, MT, and TT genotypes, respectively; p < 0.01) after adjustment for age and fat mass. An association between the AGT M235T polymorphism and adipocyte size (p < 0.02 adjusted for sex, age, and BMI) was found in another independent sample of 106 obese subjects (sex ratio, M/F 16/90; BMI, 29 to 70 kg/m(2)).


CONCLUSIONS
In cultured adipocytes from obese subjects, AGT secretion was not associated with body fat phenotypes, blood pressure, or fat cell size. However, results from two independent studies suggest an association between the AGT M235T polymorphism and adipocyte size.
Prat-Larquemin
Lydie
L
Université Paris VI, Hôtel-Dieu, Parvis Notre-Dame, Paris, France.
Oppert
Jean-Michel
JM
Clément
Karine
K
Hainault
Isabelle
I
Basdevant
Arnaud
A
Guy-Grand
Bernard
B
Quignard-Boulangé
Annie
A
D000005:Abdomen; D017667:Adipocytes / Q000473:pathology / Q000502:physiology*; D000328:Adult; D000808:Angiotensinogen / Q000235:genetics* / Q000378:metabolism*; D001706:Biopsy; D001794:Blood Pressure; D001823:Body Composition; D048429:Cell Size; D002478:Cells, Cultured; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D009765:Obesity / Q000235:genetics / Q000503:physiopathology*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D000808:Angiotensinogen