title: The W303 genetic background affects the isw2 delta mutant phenotype in Saccharomyces cerevisiae.
abstract: We performed detailed phenotypic analysis of the isw2 delta strains of the W303 genetic background and compared its results with those obtained previously in BY-derived genetic background. Shmoolike morphology was observed in the isw2 delta strain of alpha-mating type of the BY strains, but not in its W303-derived counterpart. On the other hand, derepression of a-specific genes in the isw2 delta (MAT alpha) strain was observed in both genetic backgrounds, although to a different extent. Unlike in BY-derived strain hyperactivation of the Ras2/cAMP pathway reduced invasiveness of the isw2 delta strain (MAT alpha) of the W303 background. Sensitivity to Calcofluor White indicating a cell wall-integrity defect was significantly increased in the isw2 delta strains of the W303 background in contrast to BY-derived strains. Our data indicate that the effects of the isw2 deletion strongly depend on the background in which the deletion, is made.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia.
authors.forename: P
authors.initials: P
authors.lastname: Trachtulcová
authors.forename: I
authors.initials: I
authors.lastname: Frýdlová
authors.forename: I
authors.initials: I
authors.lastname: Janatová
authors.forename: A
authors.initials: A
authors.lastname: Dorosh
authors.forename: J
authors.initials: J
authors.lastname: Hasek
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D002473:Cell Wall; D017353:Gene Deletion; D005838:Genotype; D006238:Haploidy; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: C086468:ISWI protein; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000251:Adenosine Triphosphatases