NSSR1 promotes neuronal differentiation of mouse embryonic carcinoma P19 cells.
We generated the small interference RNAs to specifically silence the expression of neural salient serine/arginine rich protein 1 (NSSR1) and showed that the inhibition of NSSR1 expression in mouse embryonic carcinoma cells (P19) reduces neuronal differentiation. By contrast, its over-expression promotes the differentiation. Neither inhibition nor over-expression shows distinct effect on cell proliferation. The over-expression increases the inclusion of NCAM L1 exon2 while the inhibition reduces the inclusion. The splicing of kinase insert free isoform of TrkC (TrkC-K1) is increased by the over-expression. The results demonstrate that NSSR1 promotes neuronal differentiation and the splicing of NCAML1 exon2 and TrkC-K1.
Liu
Lei
L
Laboratory of Genomic Physiology, Center for Brain Research; National Key Laboratory of Medical Neuroscience, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
Chen
Xian-hua
XH
Huang
Jia
J
Lin
Jun-ji
JJ
Lin
Wan-min
WM
Xu
Ping
P
D017398:Alternative Splicing; D000818:Animals; D015152:Blotting, Northern / Q000379:methods; D015153:Blotting, Western / Q000379:methods; D001973:Bromodeoxyuridine / Q000378:metabolism; D002277:Carcinoma; D002352:Carrier Proteins / Q000502:physiology*; D002452:Cell Count; D018797:Cell Cycle Proteins; D002454:Cell Differentiation / Q000502:physiology*; D045744:Cell Line, Tumor; D004622:Embryo, Mammalian; D005091:Exons; D015870:Gene Expression / Q000187:drug effects; D005786:Gene Expression Regulation; D051379:Mice; D009363:Neoplasm Proteins / Q000502:physiology*; D019006:Neural Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000473:pathology*; D012333:RNA, Messenger / Q000096:biosynthesis; D034741:RNA, Small Interfering / Q000494:pharmacology; D016601:RNA-Binding Proteins / Q000502:physiology*; D020812:Receptor, trkC / Q000235:genetics / Q000378:metabolism; D011963:Receptors, GABA-A / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014162:Transfection / Q000379:methods; D014404:Tubulin / Q000378:metabolism
D002352:Carrier Proteins; D018797:Cell Cycle Proteins; C485446:Fusip1 protein, mouse; D009363:Neoplasm Proteins; D019006:Neural Cell Adhesion Molecules; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D011963:Receptors, GABA-A; D012097:Repressor Proteins; D014404:Tubulin; D020812:Receptor, trkC; D001973:Bromodeoxyuridine