[Physiological and biochemical analysis of the transformants of aerobic methylobacteria expressing the dcm A gene of dichloromethane dehydrogenase].
The transformants of Methylobacterium dichloromethanicum DM4 (DM4-2cr-/pME8220 and DM4-2cr-/pME8221) and of Methylobacterium extorquens AM1 (AM1/pME8220 and AM1/pME8221) that express the dcm A gene of dichloromethane dehalogenase undergo lysis when incubated in the presence of dichloromethane and are sensitive to acidic shock. The lysis of the transformants was found to be related neither to the accumulation of Cl- ions, CH2O, and HCOOH, nor to the impairment of glutathione synthesis or to the maintenance of intracellular pH. The (exo-) Klenow fragment-mediated incorporation of [alpha-32P]dATP into the DNA of the transformants DM4-2cr-/pME8220 and AM1/pME8220 was considerably greater when the transformed cells were incubated with CH2Cl2 than when they were incubated with CH3OH, indicating the occurrence of a significant increase in the total length of gaps. At the same time, the strain AM1 (which lacks dichloromethane dehalogenase) and the dichloromethane-degrading strain DM4 incubated with CH2Cl2 showed an insignificant increase in the total length of the gaps. The transformed cells are likely to lyse due to the relatively inefficient repair of DNA lesions that are induced in response to the alkylating action of S-chloromethylglutathione, an intermediate product of CH2Cl2 degradation. The data obtained suggest that the bacterial mineralization of dichloromethane requires an efficient DNA repair system.
Firsova
Iu E
IuE
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow Oblast, 142290 Russia.
Doronina
N V
NV
Trotsenko
Iu A
IuA
D000143:Acids; D001433:Bacteriolysis; D004260:DNA Repair; D004269:DNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D003838:Deoxyadenine Nucleotides / Q000737:chemistry / Q000378:metabolism; D005978:Glutathione / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D008190:Lyases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000432:Methanol / Q000378:metabolism; D008752:Methylene Chloride / Q000378:metabolism; D020580:Methylobacterium / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D010761:Phosphorus Radioisotopes; D014169:Transformation, Bacterial
D000143:Acids; D004269:DNA, Bacterial; D003838:Deoxyadenine Nucleotides; D010761:Phosphorus Radioisotopes; C085664:S-chloromethylglutathione; D008752:Methylene Chloride; D008190:Lyases; C045224:dichloromethane dehalogenase; D005978:Glutathione; C026600:2'-deoxyadenosine triphosphate; D000432:Methanol