The induction of intercellular adhesion molecule-1 on human umbilical vein endothelial cells by a heat-stable component of Porphyromonas gingivalis.
Live Porphyromonas gingivalis enhanced the expression of intercellular adhesion molecule-1 (ICAM-1) on the surface of human umbilical vein endothelial cells (HUVECs) in a bacterial dose-dependent manner. Inactivation of P. gingivalis by ultraviolet (UV), heat (56 degrees C, 30 min), or sonication did not alter its stimulatory activity. ICAM-1 expression began to increase at 4 h after stimulation, reached a maximum at 12 h, and remained at the maximum for at least the next 8 h. This time course was similar to that of expression by Escherichia coli LPS. Furthermore, the effect of UV-inactivated P. gingivalis was not inhibited by boiling or polymyxin B treatment. In addition, the effect of P. gingivalis strain W83 on ICAM-1 expression was stronger than that of strain ATCC 33277. Our results suggested that some unidentified, heat-stable proteins, polysaccharides, or lipids may be the stimulatory factor(s), although the participation of LPS could not be completely ruled out. The ability of P. gingivalis to stimulate ICAM-1 expression on endothelial cells may play an important role in the pathogenesis of periodontal disease.
Mao
Song
S
Department of Bacteriology, Faculty of Medicine, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-8520, Japan.
Maeno
Nobuaki
N
Matayoshi
Seiken
S
Yoshiie
Kiyotaka
K
Fujimura
Tsuyoshi
T
Oda
Hiroshi
H
D001426:Bacterial Proteins / Q000276:immunology; D002478:Cells, Cultured; D015169:Colony Count, Microbial; D042783:Endothelial Cells / Q000378:metabolism* / Q000382:microbiology*; D005786:Gene Expression Regulation; D006358:Hot Temperature; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D007700:Kinetics; D008055:Lipids / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D011112:Polymyxin B / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000276:immunology; D016966:Porphyromonas gingivalis / Q000737:chemistry* / Q000254:growth & development / Q000472:pathogenicity*; D013010:Sonication; D014466:Ultraviolet Rays; D014471:Umbilical Veins
D001426:Bacterial Proteins; D008055:Lipids; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D018799:Intercellular Adhesion Molecule-1; D011112:Polymyxin B