Isolation and inheritance of microsatellite loci in the Dungeness crab (Brachyura: Cancridae: Cancer magister).
The isolation, PCR amplification, and descriptive statistics of six microsatellite loci are described for the Dungeness crab, Cancer magister. Also reported is the inheritance of these loci in two families obtained from artificial crosses in the laboratory. All six loci conform to expectations under Mendelian inheritance and there is no evidence for linkage between any of the loci. Allelic size ranges for three of the loci are relatively large, ranging from 135-357 bp between the smallest and largest allele detected at that locus. At two of these loci upper allelic drop out (non-amplification of the larger allele in a heterozygous individual) can be problematic for scoring. Results from cross-species amplification in nine congeners are summarized. These loci will be valuable in studies requiring high-resolution genetic markers in Dungeness crabs and related species.
Jensen
Pamela C
PC
Marine Molecular Biotechnology Laboratory, School of Aquatic and Fishery Sciences, University of Washington, Seattle 98105, USA. pam.jensen@noaa.gov
Bentzen
Paul
P
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D003386:Brachyura / Q000235:genetics*; D015698:Genomic Library; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction