title: Production of highly 13C-labeled polyphenols in Vitis vinifera cell bioreactor cultures.
abstract: The use of Vitis vinifera cells grown in a 2 l-stirred tank bioreactor for producing isotopically 13C-labeled phenolic substances is presented. Several culture parameters were optimized to achieve characteristics of growth and polyphenol metabolism similar to that recorded in shake flasks. Administration of [1-13C]L-phenylalanine (3 mM) to grape cell suspension cultures led to the production of 13C-labeled stilbenes (trans- and cis-piceids), catechins (catechin and epicatechin) and anthocyanins (delphinidin-, cyanidin-, petunidin-, peonidin- and malvidin-3-O-beta-glucosides). Incorporation of [1-13C]L-phenylalanine into polyphenols was measured by means of 13C satellites in the proton NMR spectrum and EA-IRMS. The enrichment of labeling obtained for all the compounds (between 40 and 65%) is sufficient to investigate their absorption and metabolism in humans.
authors.affiliation: Groupe d'Etudes des Substances Végétales à Activité Biologique, EA 3675, Université de Bordeaux 2, Faculté des Sciences Pharmaceutiques, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Aumont
authors.forename: Fabienne
authors.initials: F
authors.lastname: Larronde
authors.forename: Tristan
authors.initials: T
authors.lastname: Richard
authors.forename: Hélène
authors.initials: H
authors.lastname: Budzinski
authors.forename: Alain
authors.initials: A
authors.lastname: Decendit
authors.forename: Gérard
authors.initials: G
authors.lastname: Deffieux
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Krisa
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Mérillon
mesh_terms: D019149:Bioreactors; D002247:Carbon Isotopes; D005419:Flavonoids / Q000096:biosynthesis*; D007553:Isotope Labeling / Q000379:methods*; D010636:Phenols; D010649:Phenylalanine / Q000378:metabolism; D059808:Polyphenols; D013267:Stilbenes / Q000378:metabolism; D027843:Vitis / Q000166:cytology* / Q000378:metabolism*
chemical_list: D002247:Carbon Isotopes; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D013267:Stilbenes; D010649:Phenylalanine