title: Virulent Burkholderia pseudomallei is more efficient than avirulent Burkholderia thailandensis in invasion of and adherence to cultured human epithelial cells.
abstract: Burkholderia pseudomallei, a causative agent of melioidosis, is a facultative intracellular gram-negative bacillus that is closely related to its avirulent counterpart, Burkholderia thailandensis. However, pathogenic mechanisms and virulence factors of B. pseudomallei remain elusive. In the present study, we compared the invasiveness, adherence, and replication of B. pseudomallei and B. thailandensis in human respiratory epithelial cells A549. Invasion was determined after 4 h of coculturing using antibiotic protection assay. Adherence was demonstrated by coculturing the cells with fluorescein-labeled bacteria for 1 h and the number of positive cells was analyzed by flow cytometry. The results obtained with this in vitro study demonstrated that compared with its avirulent counterpart, B. pseudomallei is significantly more efficient (P<0.01) in invasion, adherence and inducing cellular damage, as represented by plaque formation.
authors.affiliation: Laboratory of Immunology, Chulabhorn Research Institute, Bangkok, Thailand.
authors.forename: Wannapa
authors.initials: W
authors.lastname: Kespichayawattana
authors.forename: Pakamas
authors.initials: P
authors.lastname: Intachote
authors.forename: Pongsak
authors.initials: P
authors.lastname: Utaisincharoen
authors.forename: Stitaya
authors.initials: S
authors.lastname: Sirisinha
mesh_terms: D001422:Bacterial Adhesion; D019117:Burkholderia / Q000254:growth & development / Q000472:pathogenicity* / Q000502:physiology*; D016957:Burkholderia pseudomallei / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology*; D006801:Humans; D014774:Virulence