title: Identification of a P2-related prophage remnant locus of Photorhabdus luminescens encoding an R-type phage tail-like particle.
abstract: Analysis of the Photorhabdus luminescens genome sequence revealed that the pts region is related to the tail synthesis gene core of the P2 phage. The pts locus encodes a DNA invertase homologue. PCR-RFLP analysis showed the two potential tail fiber regions of the pts locus present DNA inversions. Electron microscopy revealed a phage tail-like particle, related to the R-type family and named R-photorhabdicin, in the culture supernatant of P. luminescens. Mass spectrometry analysis of two sub-units of R-photorhabdicin revealed that they are encoded by the pts locus. The role of this P2-related prophage remnant in the Photorhabdus genome is discussed.
authors.affiliation: Laboratoire EMIP, Université Montpellier II, IFR122, Institut National de la Recherche Agronomique (UMR 1133), 34095 Montpellier Cedex 5, France. sgaudriault@crit.univ-montp2fr
authors.forename: Sophie
authors.initials: S
authors.lastname: Gaudriault
authors.forename: Jacques-Olivier
authors.initials: JO
authors.lastname: Thaler
authors.forename: Eric
authors.initials: E
authors.lastname: Duchaud
authors.forename: Frank
authors.initials: F
authors.lastname: Kunst
authors.forename: Noël
authors.initials: N
authors.lastname: Boemare
authors.forename: Alain
authors.initials: A
authors.lastname: Givaudan
mesh_terms: D017901:Bacteriophage P2 / Q000201:enzymology / Q000235:genetics* / Q000648:ultrastructure; D008854:Microscopy, Electron; D020637:Photorhabdus / Q000235:genetics* / Q000821:virology*; D039002:Prophages / Q000201:enzymology / Q000235:genetics / Q000648:ultrastructure; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D043324:beta-Fructofuranosidase / Q000235:genetics* / Q000378:metabolism
chemical_list: D043324:beta-Fructofuranosidase