title: [Genotyping of EsBLs-producing Escherichia coli by pulsed-field gel electrophoresis in nosocomial infection].
abstract: OBJECTIVE
  Using a genotying method which is accurate and rapid to study the molecular epidemiology of EsBLs-producing Escherichia coli in nosocomial infection.
  
  
  METHODS
  After an analysis of clinical information to ascertain nosocomial infection, EsBLs were detected by phenotype confirmation test and pulsed-field gel electrophoresis (PFGE).
  
  
  RESULTS
  Three EsBLs-producing strains in ICU were verified as epidemiological-related because their dice coefficient index was higher than 95%. The others were verified as non-epidemiological-related since their different lanes were more than 6.
  
  
  CONCLUSIONS
  PFGE is a perfect molecular typing method to study epidemiolgy and analyze homology.
authors.affiliation: Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Kang
authors.forename: Chao-yang
authors.initials: CY
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Norman
authors.forename: Wen-zhao
authors.initials: WZ
authors.lastname: Chen
authors.forename: Xiao-jing
authors.initials: XJ
authors.lastname: Guo
authors.forename: Dong-bin
authors.initials: DB
authors.lastname: Zheng
mesh_terms: D002681:China / Q000453:epidemiology; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology / Q000235:genetics; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D010641:Phenotype; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001618:beta-Lactamases