Alteration of plasma total F2-isoprostanes before and after hemodialysis in end-stage renal disease patients.
F(2)-isoprostanes are derived in vivo principally from the following: (1) the formation of positional peroxyl radicals of arachidonic acid, (2) endocyclization to prostaglandin G(2)-like structures, and (3) reduction to PGF(2)-like compounds. F(2)-isoprostanes have been proposed as biomarkers of lipid peroxidation, oxidative stress status, and the oxidation of low-density lipoprotein (LDL). Using gas chromatography-ion trap-mass spectrometry, we studied how hemodialysis (HD) affects plasma total F(2)-isoprostanes. We examined the plasma total F(2)-isoprostanes in end-stage renal disease (ESRD) patients, before HD, after HD, between HD, and in control subjects. Plasma concentrations of total F(2)-isoprostanes were significantly higher in the after HD ESRD patients than the before hemodialysis ESRD patients (P < 0.05). There is no difference between before HD ESRD patients and normal controls. Moreover, a positive or negative correlation was seen between LDL and plasma total F(2)-isoprostanes (P < 0.001), and between age and plasma total F(2)-isoprostanes (P < 0.001). This study indicates HD treatment may be the major contributor of oxidative stress in ESRD patients.
Kim
Kyung Mee
KM
Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, P.O. Box 131, Seoul 130-650, South Korea.
Jung
Byung Hwa
BH
Paeng
Ki-Jung
KJ
Kim
Sang Wook
SW
Chung
Bong Chul
BC
D016718:Arachidonic Acid / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D028441:F2-Isoprostanes / Q000097:blood*; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000503:physiopathology / Q000628:therapy; D015227:Lipid Peroxidation / Q000502:physiology; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology; D011237:Predictive Value of Tests; D006435:Renal Dialysis
D015415:Biomarkers; D028441:F2-Isoprostanes; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D016718:Arachidonic Acid