Validation of a simple and inexpensive method for the quantitation of infarct in the rat brain.
A gravimetric method was evaluated as a simple, sensitive, reproducible, low-cost alternative to quantify the extent of brain infarct after occlusion of the medial cerebral artery in rats. In ether-anesthetized rats, the left medial cerebral artery was occluded for 1, 1.5 or 2 h by inserting a 4-0 nylon monofilament suture into the internal carotid artery. Twenty-four hours later, the brains were processed for histochemical triphenyltetrazolium chloride (TTC) staining and quantitation of the schemic infarct. In each TTC-stained brain section, the ischemic tissue was dissected with a scalpel and fixed in 10% formalin at 0 masculine C until its total mass could be estimated. The mass (mg) of the ischemic tissue was weighed on an analytical balance and compared to its volume (mm(3)), estimated either by plethysmometry using platinum electrodes or by computer-assisted image analysis. Infarct size as measured by the weighing method (mg), and reported as a percent (%) of the affected (left) hemisphere, correlated closely with volume (mm(3), also reported as %) estimated by computerized image analysis (r = 0.88; P < 0.001; N = 10) or by plethysmography (r = 0.97-0.98; P < 0.0001; N = 41). This degree of correlation was maintained between different experimenters. The method was also sensitive for detecting the effect of different ischemia durations on infarct size (P < 0.005; N = 23), and the effect of drug treatments in reducing the extent of brain damage (P < 0.005; N = 24). The data suggest that, in addition to being simple and low cost, the weighing method is a reliable alternative for quantifying brain infarct in animal models of stroke.
Schilichting
C L R
CL
Departamento de Farmácia e Farmacologia, Centro de Ciências da Saúde, Universidade Estadual de Maringá, Maringa, PR, Brazil.
Lima
K C M
KC
Cestari
L A
LA
Sekiyama
J Y
JY
Silva
F M
FM
Milani
H
H
D000818:Animals; D002544:Cerebral Infarction / Q000473:pathology*; D004396:Coloring Agents; D004195:Disease Models, Animal; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology; D008297:Male; D009929:Organ Size; D010991:Plethysmography; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D013194:Staining and Labeling / Q000191:economics / Q000379:methods*; D013778:Tetrazolium Salts
D004396:Coloring Agents; D013778:Tetrazolium Salts; C009591:triphenyltetrazolium