Identification of murine and human XCP1 genes as C/EBP-epsilon-dependent members of FIZZ/Resistin gene family.
The CCAAT enhancer binding protein epsilon (C/EBP-epsilon) transcription factor is expressed predominantly in granulocytes. Mice with a disruption of the C/EBP-epsilon gene fail to produce mature granulocytes and eosinophils. Cells derived from the peritoneal exudates of C/EBP-epsilon -/- mice lack the expression of a number of chemokines and chemokine receptor genes. We have found a novel C/EBP-epsilon-dependent promyelocyte-specific gene, mXCP1. mXCP1 belongs to a family of XCP/FIZZ/Resistin genes, which includes four murine genes and two human genes, hXCP1 and hXCP2. These genes have four exons and encode short secreted proteins sharing a ten-cysteine motif. Murine mXCP1, mXCP2 and mXCP3 genes map to murine chromosome 16 and mXCP4 is positioned on chromosome 8; the hXCP1 and hXCP2 genes are located at homologous regions of chromosomes 3 and 19. Introduction of an inducible C/EBP-epsilon gene into the NIH3T3 and myeloid cells from C/EBP-epsilon-null mice line revealed that the conditional expression of C/EBP-epsilon induced mXCP1. The HXCP1 gene was identified as a C/EBP-epsilon-dependent regulatory homologue of mXCP1. The expression data for other members of XCP/FIZZ gene family are presented. Further studies indicate that XCP1 is a secreted protein that is chemotactic to myeloid cells from C/EBP-epsilon-null mice and is able to interact directly with alpha-defensin.
Chumakov
Alexey M
AM
Cedars-Sinai Medical Center, Burns & Allen Research Institute, Division of Hematology/Oncology, UCLA School of Medicine, Los Angeles, CA 90048, USA. achumakov@hotmail.com
Kubota
Tetsuya
T
Walter
Steffen
S
Koeffler
H Phillip
HP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D022762:CCAAT-Enhancer-Binding Proteins / Q000502:physiology*; D002633:Chemotaxis; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000032:analysis; D006729:Hormones, Ectopic / Q000235:genetics*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D008969:Molecular Sequence Data; D020932:Nerve Growth Factor / Q000235:genetics*; D009928:Organ Specificity; D011506:Proteins; D052243:Resistin; D023084:alpha-Defensins / Q000378:metabolism
D022762:CCAAT-Enhancer-Binding Proteins; C489468:Cebpe protein, mouse; D018076:DNA, Complementary; D006729:Hormones, Ectopic; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins; C499624:RETN protein, human; C486574:RETNLB protein, human; D052243:Resistin; C499623:Retn protein, mouse; C413576:Retnla protein, mouse; D023084:alpha-Defensins; C070790:CEBPE protein, human; D020932:Nerve Growth Factor