title: Controlled fed-batch fermentations of dilute-acid hydrolysate in pilot development unit scale.
abstract: Inhibitors formed during wood hydrolysis constitute a major problem in fermenting dilute-acid hydrolysates. By applying a fed-batch technique, the levels of inhibitory compounds may be held low, enabling high ethanol productivity. In this study, a previously developed fed-batch strategy was modified and implemented for use in pilot development unit (PDU) scale. The rate of total gas formation, measured with a mass flow meter, was used as input variable in the control algorithm. The feed rate in the PDU-scale experiments could be properly controlled based on the gas evolution from the reactor. In fed-batch experiments utilizing TMB 3000, an inhibitor-tolerant strain of Saccharomyces cerevisiae, close to 100% of the hexoses in the hydrolysate was converted.
authors.affiliation: Chemical Engineering, Lund University, Box 124, SE-221 00 Lund, Sweden. andreas.rudolf@chemeng.lth.se
authors.forename: Andreas
authors.initials: A
authors.lastname: Rudolf
authors.forename: Mats
authors.initials: M
authors.lastname: Galbe
authors.forename: Gunnar
authors.initials: G
authors.lastname: Lidén
mesh_terms: D000143:Acids; D000465:Algorithms; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002245:Carbon Dioxide / Q000737:chemistry; D002470:Cell Survival; D002482:Cellulose / Q000737:chemistry*; D000431:Ethanol; D005285:Fermentation; D006601:Hexoses / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D015233:Models, Statistical; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013997:Time Factors; D014934:Wood
chemical_list: D000143:Acids; D006601:Hexoses; D002245:Carbon Dioxide; D000431:Ethanol; D002482:Cellulose