Performance of a newly developed integrant of Zymomonas mobilis for ethanol production on corn stover hydrolysate.
Efficient conversion of lignocellulosic biomass requires biocatalysts able to tolerate inhibitors produced by many pretreatment processes. Recombinant Zymomonas mobilis 8b, a recently developed integrant of Zymomonas mobilis 31821(pZB5), tolerated acetic acid up to 16 g l(-1) and achieved 82%-87% (w/w) ethanol yields from pure glucose/xylose solutions at pH 6 and temperatures of 30 degrees C and 37 degrees C. An ethanol yield of 85% (w/w) was achieved on glucose/xylose from hydrolysate produced by dilute sulfuric acid pretreatment of corn stover after an overliming' process was used to improve hydrolysate fermentability.
Mohagheghi
Ali
A
National Bioenergy Center National Renewable Energy Laboratory, Golden, CO 80401, USA. ali_mohagheghi@nrel.gov
Dowe
Nancy
N
Schell
Daniel
D
Chou
Yat-Chen
YC
Eddy
Christina
C
Zhang
Min
M
D000085:Acetates / Q000737:chemistry; D019342:Acetic Acid / Q000737:chemistry; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002482:Cellulose / Q000737:chemistry; D003470:Culture Media / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008031:Lignin / Q000737:chemistry; D011767:Pyruvate Decarboxylase / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D013464:Sulfuric Acids / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors; D014994:Xylose / Q000378:metabolism; D003313:Zea mays / Q000378:metabolism*; D017355:Zymomonas / Q000378:metabolism*
D000085:Acetates; D003470:Culture Media; D011994:Recombinant Proteins; D013464:Sulfuric Acids; C036909:lignocellulose; D000431:Ethanol; D002482:Cellulose; D008031:Lignin; D014994:Xylose; D011767:Pyruvate Decarboxylase; D005947:Glucose; C033158:sulfuric acid; D019342:Acetic Acid