title: Determination of acrylamide and glycidamide serum toxicokinetics in B6C3F1 mice using LC-ES/MS/MS.
abstract: Acrylamide (AA) is a well-studied industrial toxicant; however, recent findings of AA at ppm levels in cooked starchy foods have refocused attention on the potential for neurotoxicity, germ cell mutagenicity, and carcinogenicity from AA. Oxidative metabolism of AA to glycidamide (GA) in experimental animals has previously been linked with many toxic effects of AA exposure. We report a new sensitive and selective analytical method, based on LC with electrospray tandem mass spectrometry, for the quantification of AA and GA in serum and its application to a preliminary toxicokinetic evaluation of AA and GA in adult B6C3F(1) mice following oral administration of AA.
authors.affiliation: Division of Biochemical Toxicology, National Center for Toxicological Research, 3900 NCTR Road, Jefferson, AR 72079, USA.
authors.forename: Nathan C
authors.initials: NC
authors.lastname: Twaddle
authors.forename: L Patrice
authors.initials: LP
authors.lastname: McDaniel
authors.forename: Gonçalo
authors.initials: G
authors.lastname: Gamboa da Costa
authors.forename: Mona I
authors.initials: MI
authors.lastname: Churchwell
authors.forename: Frederick A
authors.initials: FA
authors.lastname: Beland
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Doerge
mesh_terms: D020106:Acrylamide / Q000493:pharmacokinetics*; D000284:Administration, Oral; D000818:Animals; D002853:Chromatography, Liquid; D004852:Epoxy Compounds / Q000493:pharmacokinetics*; D007700:Kinetics; D051379:Mice; D008956:Models, Chemical; D009153:Mutagens; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013997:Time Factors
chemical_list: D004852:Epoxy Compounds; D009153:Mutagens; D020106:Acrylamide; C071834:glycidamide; D010100:Oxygen