title: Assessment of copper toxicity using an acoustic wave sensor.
abstract: A piezoelectric quartz crystal microbalance has been shown to be useful to monitor real time bacterial growth. Monitoring bacterial growth can give an insight into the ecosystem, as it is highly affected by the presence of toxic elements or nutrients. The frequency of an uncoated piezoelectric quartz crystal was monitored while in contact with bacteria, isolated from water sampled from a Portuguese lagoon, growing in two different media: a saline nutrient broth (NM) and the natural water. The sensor was used to evaluate the effect of copper on bacterial growth. Copper concentrations up to 18.8 microg l(-1) showed an increase in bacterial growth in NM, and a decrease beyond 25.0 microg l(-1). Copper added to the natural water had negative effects on bacterial growth beyond 18.8 microg l(-1). Copper concentrations in the natural water from the lagoon were determined using a similar quartz crystal to detect the mass deposited by anodic stripping voltammetry, and was found to be 3.38 +/- 0.09 microg l(-1).
authors.affiliation: Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
authors.forename: Alzira
authors.initials: A
authors.lastname: Yamasaki
authors.forename: M Angela S D A
authors.initials: MA
authors.lastname: Cunha
authors.forename: João A B P
authors.initials: JA
authors.lastname: Oliveira
authors.forename: Armando C
authors.initials: AC
authors.lastname: Duarte
authors.forename: M Teresa S R
authors.initials: MT
authors.lastname: Gomes
mesh_terms: D000162:Acoustics; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development; D015374:Biosensing Techniques / Q000379:methods*; D003300:Copper / Q000494:pharmacology / Q000633:toxicity*; D011791:Quartz; D013997:Time Factors
chemical_list: D011791:Quartz; D003300:Copper