title: Ebola virus glycoprotein-mediated anoikis of primary human cardiac microvascular endothelial cells.
abstract: Ebola virus glycoprotein (EGP) has been implicated for the induction of cytotoxicity and injury in vascular cells. On the other hand, EGP has also been suggested to induce massive cell rounding and detachment from the plastic surface by downregulating cell adhesion molecules without causing cytotoxicity. In this study, we have examined the cytotoxic role of EGP in primary endothelial cells by transduction with a replication-deficient recombinant adenovirus expressing EGP (Ad-EGP). Primary human cardiac microvascular endothelial cells (HCMECs) transduced with Ad-EGP displayed loss of cell adhesion from the plastic surface followed by cell death. Transfer of conditioned medium from EGP-transduced HCMEC into naive cells did not induce loss of adhesion or cell death, suggesting that EGP needs to be expressed intracellularly to exert its cytotoxic effect. Subsequent studies suggested that HCMEC death occurred through apoptosis. Results from this study shed light on the EGP-induced anoikis in primary human cardiac endothelial cells, which may have significant pathological consequences.
authors.affiliation: Department of Pathology, Saint Louis University, St. Louis, MO 63110, USA. rayrb@slu.edu
authors.forename: Ratna B
authors.initials: RB
authors.lastname: Ray
authors.forename: Arnab
authors.initials: A
authors.lastname: Basu
authors.forename: Robert
authors.initials: R
authors.lastname: Steele
authors.forename: Aster
authors.initials: A
authors.lastname: Beyene
authors.forename: Jane
authors.initials: J
authors.lastname: McHowat
authors.forename: Keith
authors.initials: K
authors.lastname: Meyer
authors.forename: Asish K
authors.initials: AK
authors.lastname: Ghosh
authors.forename: Ranjit
authors.initials: R
authors.lastname: Ray
mesh_terms: D023102:Anoikis; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000473:pathology* / Q000821:virology; D029043:Ebolavirus / Q000378:metabolism / Q000472:pathogenicity*; D042783:Endothelial Cells / Q000473:pathology* / Q000821:virology; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014161:Transduction, Genetic; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D008562:Membrane Glycoproteins; D014763:Viral Matrix Proteins; C110614:transmembrane glycoprotein, Ebola virus