title: Reactivation of porcine cytomegalovirus through allogeneic stimulation.
abstract: Reactivation of latent porcine cytomegalovirus after coculture of peripheral blood mononuclear cells (PBMCs) from pigs with different genetic backgrounds was investigated. Nine of 10 allogeneic coculture pairs were PCR (DNA) positive, whereas 7 coculture pairs had porcine cytomegalovirus (PCMV) RNA, an indication of virus replication. The cell subpopulations harboring PCMV were monocytes and CD8+ T cells.
authors.affiliation: Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota 55108, USA.
authors.forename: Maria Isabel M C
authors.initials: MI
authors.lastname: Guedes
authors.forename: Jack M
authors.initials: JM
authors.lastname: Risdahl
authors.forename: Barry
authors.initials: B
authors.lastname: Wiseman
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Molitor
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018920:Coculture Techniques; D003587:Cytomegalovirus / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D007963:Leukocytes, Mononuclear / Q000502:physiology / Q000821:virology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine / Q000821:virology*; D014184:Transplantation, Homologous; D014775:Virus Activation; D017735:Virus Latency
chemical_list: D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral