Hydrolysis of Nothogenia erinacea xylan by xylanases from families 10 and 11.
The structures of several enzymatic hydrolysis products of Nothogenia erinacea seaweed xylan, a linear homopolymer with mixed beta-(1-->3)/beta-(1-->4) linkages, were analysed by physicochemical and biochemical techniques. With the glycoside hydrolase family 10 beta-(1-->4)-xylanase from Cryptococcus adeliae, hydrolysis proceeds to a final mixture of products containing a mixed linkage-type triose as a major compound, whereas with the family 11 xylanase from Thermomyces lanuginosus this is a mixed linkage tetraose. The Cryptococcus xylanase is shown to be capable of also catalysing the hydrolysis of beta-(1-->3) linkages, that is this of a mixed type tetraose intermediary formed, in accordance with the broader substrate specificity of family 10 enzymes. From a partial degradation experiment with the T. lanuginosus xylanase, a series of higher mixed oligosaccharides were isolated and identified. The observed oligosaccharide intermediates and splicing pattern indicate an irregular beta-(1-->3)/beta-(1-->4) linkage distribution within the linear d-xylose polymer. Similar results were obtained with rhodymenan, the seaweed xylan from Palmares palmata.
Nerinckx
Wim
W
Department of Biochemistry, Physiology and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium. wim.nerinckx@ugent.be
Broberg
Anders
A
Duus
Jens Ø
JØ
Ntarima
Patricia
P
Parolis
Lesley A S
LA
Parolis
Haralambos
H
Claeyssens
Marc
M
D001203:Ascomycota / Q000201:enzymology; D002240:Carbohydrate Sequence; D003454:Cryptococcus / Q000201:enzymology; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D011522:Protons; D000461:Rhodophyta / Q000378:metabolism*; D012624:Seaweed / Q000737:chemistry; D013379:Substrate Specificity; D013997:Time Factors; D014269:Trifluoroacetic Acid / Q000737:chemistry; D014990:Xylans / Q000737:chemistry*
D009844:Oligosaccharides; D011134:Polysaccharides; D011522:Protons; D014990:Xylans; D014269:Trifluoroacetic Acid; D043364:Endo-1,4-beta Xylanases