title: The inhibition of cell spreading on a cellulose substrate (cuprophan) induces an apoptotic process via a mitochondria-dependent pathway.
abstract: Cell shape was found to be a strong indicator of whether individual cells grow or die, and may play an important role in controlling apoptosis as well as cell growth. We compared here the behaviour of rounded Swiss 3T3 cells aggregated on a cellulose cuprophan membrane to those cultured on dish polystyrene. We demonstrated that cells aggregated on cellulose substrates for up to 48 h underwent programmed cell death that was associated with phosphatidylserine flipping and caspase 9 and caspase 3 activation, suggesting a mitochondria-dependent apoptotic process. In addition, we found that this phenomenon cannot be entirely explained by disengagement of alpha 5 beta 1 integrin ligation.
authors.affiliation: Domaine Biomatériaux-Biocompatibilité, UMR CNRS 6600, Université de Technologie de Compiègne, P.O. Box 20529, F-60205 Compiègne Cedex, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Gekas
authors.forename: Mathilde
authors.initials: M
authors.lastname: Hindié
authors.forename: Nathalie
authors.initials: N
authors.lastname: Faucheux
authors.forename: Olivia
authors.initials: O
authors.lastname: Lanvin
authors.forename: Cécile
authors.initials: C
authors.lastname: Mazière
authors.forename: Vincent
authors.initials: V
authors.lastname: Fuentès
authors.forename: Valérie
authors.initials: V
authors.lastname: Gouilleux-Gruart
authors.forename: Bertrand
authors.initials: B
authors.lastname: David
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Mazière
authors.forename: Kaïss
authors.initials: K
authors.lastname: Lassoued
authors.forename: Marie-Danielle
authors.initials: MD
authors.lastname: Nagel
mesh_terms: D000818:Animals; D017209:Apoptosis; D001672:Biocompatible Materials; D020169:Caspases / Q000378:metabolism; D002448:Cell Adhesion; D002449:Cell Aggregation; D048429:Cell Size; D002482:Cellulose / Q000031:analogs & derivatives* / Q000378:metabolism*; D004789:Enzyme Activation; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D011137:Polystyrenes / Q000378:metabolism; D015398:Signal Transduction; D013379:Substrate Specificity; D041701:Swiss 3T3 Cells
chemical_list: D001672:Biocompatible Materials; D011137:Polystyrenes; D002482:Cellulose; C009865:cuprammonium cellulose; D020169:Caspases