Regulation of the selenoprotein SelS by glucose deprivation and endoplasmic reticulum stress - SelS is a novel glucose-regulated protein.
SelS is a newly identified selenoprotein and its gene expression is up-regulated in the liver of Psammomys obesus after fasting. We have examined whether SelS is regulated by glucose deprivation and endoplasmic reticulum (ER) stress in HepG2 cells. Glucose deprivation and the ER stress inducers tunicamycin and thapsigargin increased SelS gene expression and protein content several-fold in parallel with glucose-regulated protein 78. The overexpression of SelS increased Min6 cell resistance to oxidative stress-induced toxicity. These results indicate that SelS is a novel member of the glucose-regulated protein family and its function is related to the regulation of cellular redox balance.
Gao
Yuan
Y
Metabolic Research Unit, School of Health Sciences, Deakin University, Pigdons Road, Waurn Ponds, Vic. 3217, Australia. gao@deakin.edu.au
Feng
Helen C
HC
Walder
Ken
K
Bolton
Kristy
K
Sunderland
Terry
T
Bishara
Natalie
N
Quick
Melissa
M
Kantham
Lakshmi
L
Collier
Greg R
GR
D000595:Amino Acid Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D005947:Glucose / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016877:Oxidants / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051140:Selenoproteins; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological / Q000378:metabolism*; D019284:Thapsigargin / Q000494:pharmacology; D013997:Time Factors; D014415:Tunicamycin / Q000494:pharmacology
D004791:Enzyme Inhibitors; D018840:HSP70 Heat-Shock Proteins; D008565:Membrane Proteins; D016877:Oxidants; D011506:Proteins; D012333:RNA, Messenger; D051140:Selenoproteins; C018551:glucose-regulated proteins; D014415:Tunicamycin; D019284:Thapsigargin; D006861:Hydrogen Peroxide; D008156:Luciferases; D005947:Glucose