Microfluidic chip for high efficiency DNA extraction.
A high efficiency DNA extraction microchip was designed to extract DNA from lysed cells using immobilized beads and the solution flowing back and forth. This chip was able to increase the extraction efficiency by 2-fold when there was no serum. When serum existed in the solution, the extraction efficiency of immobilized beads was 88-fold higher than that of free beads. The extraction efficiency of the microchip was tested under different conditions and numbers of E. coli cells. When the number of E. coli cells was between 10(6) and 10(8) in 25 microl of whole blood, the extraction efficiency using immobilized beads was only slightly higher than that using free beads (10(0) to 10(1) fold). When the number of E. coli cells was in the range 10(4) to 10(6) in 25 microl of whole blood, the extraction efficiency of immobilized beads was greater than that of the free beads (10(1) to 10(2) fold). When the number of E. coli cells was lower, in the range 10(3) to 10(4) in 25 microl of whole blood, the extraction efficiency of immobilized beads was much higher than that of the free beads (10(2) to 10(3) fold). This study indicated that DNA could be efficiently extracted even when the number of bacterial cells was smaller (10(5) to 10(3)). This microfluidic extraction chip could find potential applications in rare sample genomic study.
Chung
Yung-Chiang
YC
Electronics Research & Service Organization, Industrial Technology Research Institute, Hsinchu, Taiwan.
Jan
Ming-Shiung
MS
Lin
Yu-Cheng
YC
Lin
Ju-Hwa
JH
Cheng
Wang-Chin
WC
Fan
Chia-Yu
CY
D001769:Blood; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics; D005260:Female; D006801:Humans; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods*; D008863:Microspheres; D016133:Polymerase Chain Reaction
D004269:DNA, Bacterial