In vitro evaluation of sulfadrugs against fungi causing otomycosis.
The efficacy of four sulfadrugs i.e., sulfadiazine, sulfamethoxazole, sulfaguanidine and sulfamoxole were taken in different doses (500, 1000, 1500, 2000 and 2500 ppm) to test their effectiveness against five pathogenic organisms for the control of mycelial growth and sporulation. Amongst the drugs tested sulfamethoxazole seems to be most effective against most of the test fungi showing 96.78, 90.53, 86.62, 54.16 and 43.91 percent inhibition in mycelial growth of P. nigricans, A. flavus, A. corymbifera, A. niger and C. albicans, respectively at its 2500 ppm dose in the basal medium. Gradual reduction in sporulation in all the test organisms almost in all the sulfadrugs have caused inhibition in sporulation, in comparison to their respective controls. Maximum inhibitory effect of sulfadiazine was recorded in A. niger showing 77.26 percent inhibition in the mycelial growth at a dose of 2500 ppm. Sulfaguanidine at its higher dose i.e., 2500 ppm proved to be most toxic against A. corymbifera causing 56.39 percent inhibition in the mycelial growth, while the same dose could not be found much effective against other test fungi. Sulfamoxole was found to be quite effective against most of the test fungi causing 47.16 to 85.86 percent inhibition in the mycelial growth. A gradual inhibition in the vegetative growth and sporulation or budding in all the test fungi was noted by increasing the concentrations of the test sulfadrugs.
Jain
Sudhir K
SK
Department of Applied Microbiology, Cancer Hospital & Research Institute, Gwalior 474 009, India. Sudhirkjain1@rediffmail.com
Agrawal
S C
SC
Jain
P C
PC
D000935:Antifungal Agents / Q000494:pharmacology*; D005658:Fungi / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D008826:Microbial Sensitivity Tests; D009181:Mycoses / Q000382:microbiology*; D010033:Otitis Media / Q000382:microbiology*; D013172:Spores, Fungal / Q000187:drug effects; D013449:Sulfonamides / Q000494:pharmacology*
D000935:Antifungal Agents; D013449:Sulfonamides