Evaluation of different methods to determine the loading of proteins in PLGA microspheres.
Various determination methods for protein encapsulation efficiency of PLGA microspheres were compared. Acetonitrile is recommended as an extraction solvent.
Zheng
C H
CH
Department of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, PR China.
Liang
W Q
WQ
Yu
H Y
HY
Chen
H L
HL
D000097:Acetonitriles; D004339:Drug Compounding / Q000379:methods*; D019344:Lactic Acid; D008752:Methylene Chloride; D008863:Microspheres; D010316:Particle Size; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012995:Solubility; D012997:Solvents
D000097:Acetonitriles; D011108:Polymers; D011506:Proteins; D012997:Solvents; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; D008752:Methylene Chloride; C032159:acetonitrile