Microwave-immobilized polybutadiene stationary phase for reversed-phase high-performance liquid chromatography.
Polybutadiene (PBD) has been immobilized on high-performance liquid chromatography (HPLC) silica by microwave radiation at various power levels (52-663 W) and actuation times (3-60 min). Columns prepared from these reversed-phase HPLC materials, as well as from similar non-irradiated materials, were tested with standard sample mixtures and characterized by elemental analysis (%C) and infrared spectroscopy. A microwave irradiation of 20 min at 663 W gives a layer of immobilized PBD that presented good performance. Longer irradiation times give thicker immobilized layers having less favorable chromatographic properties.
Lopes
Nilva P
NP
Laboratório de Pesquisas em Cromatografia Líquida, LABCROM, Instituto de Química, Universidade Estadual de Campinas, Caixa Postal 6154, CEP 13084-971 Campinas, SP, Brazil.
Collins
Kenneth E
KE
Jardim
Isabel C S F
IC
D002070:Butadienes / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D020724:Elastomers; D008872:Microwaves; D011108:Polymers / Q000737:chemistry*; D013055:Spectrophotometry, Infrared
D002070:Butadienes; D020724:Elastomers; D011108:Polymers; C028834:polybutadiene