Extracellular peroxidase production by Coprinus species from urea-treated soil.
Thirteen strains of inky-cap mushroom Coprinus species were evaluated for the production of extracellular peroxidase. The liquid fermentation was carried out in shake flasks containing 1% glucose, 0.5% peptone, 0.3% yeast extract, and 0.3% malt extract broth at 25 degrees C. Peroxidase activity was detected in the liquid culture of several Coprinus species, including C. echinosporus NBRC 30630; C. macrocephalus NBRC 30117; Coprinus spp. UAMH 10065, UAMH 10066, UAMH 10067, and 074, after 10 days of growth. Peroxidase production by Coprinus sp. UAMH 10067, a Coprinus species isolated from urea-treated soil, was comparable to that of C. cinereus and reached 15 U.mL(-1) after 10 days. In addition, the peroxidase from Coprinus sp. UAMH 10067 was apparently more thermally stable than the enzyme produced by C. cinereus.
Ikehata
Keisuke
K
Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Canada.
Buchanan
Ian D
ID
Smith
Daniel W
DW
D000675:Ampyrone / Q000378:metabolism; D018533:Biomass; D003302:Coprinus / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D042845:Laccase / Q000378:metabolism; D009195:Peroxidase / Q000096:biosynthesis*; D010636:Phenols / Q000378:metabolism
D010636:Phenols; D000675:Ampyrone; D042845:Laccase; D009195:Peroxidase; D005947:Glucose