Canine and feline hematology reference values for the ADVIA 120 hematology system.
BACKGROUND
The ADVIA 120 is a laser-based hematology analyzer with software applications for animal species. Accurate reference values would be useful for the assessment of new hematologic parameters and for interlaboratory comparisons.


OBJECTIVE
The goal of this study was to establish reference intervals for CBC results and new parameters for RBC morphology, reticulocytes, and platelets in healthy dogs and cats using the ADVIA 120 hematology system.


METHODS
The ADVIA 120, with multispecies software (version 1.107-MS), was used to analyze whole blood samples from clinically healthy dogs (n=46) and cats (n=61). Data distribution was determined and reference intervals were calculated as 2.5 to 97.5 percentiles and 25 to 75 percentiles.


RESULTS
Most data showed Gaussian or log-normal distribution. The numbers of RBCs falling outside the normocytic-normochromic range were slightly higher in cats than in dogs. Both dogs and cats had reticulocytes with low, medium, and high absorbance. Mean numbers of large platelets and platelet clumps were higher in cats compared with dogs.


CONCLUSIONS
Reference intervals obtained on the ADVIA 120 provide valuable baseline information for assessing new hematologic parameters and for interlaboratory comparisons. Differences compared with previously published reference values can be attributed largely to differences in methodology.
Moritz
Andreas
A
Clinic for Internal Medicine and Forensic Affairs, Faculty of Veterinary Medicine, Justus-Liebig-University, Giessen, Germany. andreas.moritz@vetmed.uni-giessen.de
Fickenscher
Yvonne
Y
Meyer
Karin
K
Failing
Klaus
K
Weiss
Douglas J
DJ
D000818:Animals; D001772:Blood Cell Count / Q000379:methods / Q000592:standards / Q000662:veterinary*; D002415:Cats / Q000097:blood*; D004285:Dogs / Q000097:blood*; D004906:Erythrocyte Count / Q000379:methods / Q000592:standards / Q000662:veterinary; D005260:Female; D006403:Hematologic Tests / Q000295:instrumentation / Q000379:methods / Q000592:standards / Q000662:veterinary*; D008297:Male; D016011:Normal Distribution; D010976:Platelet Count / Q000379:methods / Q000592:standards / Q000662:veterinary; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity