title: Skeletal muscle differentiation potential of human adult bone marrow cells.
abstract: Murine bone marrow (BM) cells have been shown to undergo myogenic differentiation and participate in muscle repair in different muscle regeneration models. In the present paper, we report on a subset of cells (CD45+/desmin+) with myogenic potential being present at very low frequencies in human adult BM. By a simple culture method, we were able to obtain in vitro multinucleated myotubes in up to 20% of the cultures. Myotubes were generated using both BM flushed from rib fragments obtained during thoracotomy and BM derived from iliac crest aspirates. Cells of the different adherent and non-adherent fractions expressed numerous muscle specific markers by immunocytochemistry, real-time RT-PCR, flow cytometry, and Western blot analyses. Moreover, direct injection of whole BM into the right tibialis anterior muscle of immunodeficient mice (NOD/RAG) that had previously been treated with cardiotoxin to induce muscle degeneration, showed a variable but significant level of human cell engraftment (from 0.06 to 0.26% Dys+/FISH+ fibers). These data suggest that cells with skeletal muscle differentiation potential are present in adult human BM can differentiate in vitro and give rise to myogenic cells in vivo in immunodeficient mice after muscle damage. Further improvements might allow new approaches to cell-mediated therapies for muscular diseases.
authors.affiliation: Fondazione Matarelli, Ospedale Fatebenefratelli e Oftalmico, Milan, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Bossolasco
authors.forename: Stefania
authors.initials: S
authors.lastname: Corti
authors.forename: Sandra
authors.initials: S
authors.lastname: Strazzer
authors.forename: Chiara
authors.initials: C
authors.lastname: Borsotti
authors.forename: Roberto
authors.initials: R
authors.lastname: Del Bo
authors.forename: Francesco
authors.initials: F
authors.lastname: Fortunato
authors.forename: Sabrina
authors.initials: S
authors.lastname: Salani
authors.forename: Nadia
authors.initials: N
authors.lastname: Quirici
authors.forename: Francesco
authors.initials: F
authors.lastname: Bertolini
authors.forename: Alberto
authors.initials: A
authors.lastname: Gobbi
authors.forename: Giorgio Lambertenghi
authors.initials: GL
authors.lastname: Deliliers
authors.forename: Giacomo
authors.initials: G
authors.lastname: Pietro Comi
authors.forename: Davide
authors.initials: D
authors.lastname: Soligo
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D015415:Biomarkers; D001706:Biopsy; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D007085:Ilium; D017404:In Situ Hybridization, Fluorescence; D008175:Lung Neoplasms / Q000601:surgery; D051379:Mice; D016688:Mice, Inbred NOD; D018482:Muscle, Skeletal / Q000166:cytology*; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012272:Ribs; D033581:Stem Cell Transplantation; D014183:Transplantation, Heterologous
chemical_list: D015415:Biomarkers; D000469:Alkaline Phosphatase