title: G and P genotypes of group A rotavirus strains circulating in calves in Brazil, 1996-1999.
abstract: Fifty fecal samples from calves with diarrhea, positive for group A rotavirus by enzyme immunoassay (EIA) and polyacrylamide gel electrophoresis (PAGE) were analyzed by a nested multiplex reverse transcription/polymerase chain reaction (Nested multiplex/RT-PCR) for identification of P and G genotypes. Samples were collected between 1996 and 1999 from eight dairy and/or beef cattle herds located in the Mato Grosso do Sul, São Paulo, Goiás and Paraná States, Brazil. Complete genotyping was possible in 44 (88%) of the calf fecal samples. The VP7 gene could not be identified in six (12%) of the samples. Whilst the VP4 (P) gene was identified in 100% of samples. The genotypes of 35 (70%) samples corresponded to the NCDV-like (12%), UK-like (40%), B223-like (16%) and KN-4 (2%) reference rotavirus strains, which are commonly found in cattle. Mixed infections were detected in seven (14%) samples, and genotypes observed in two (4%) samples presented unusual combinations, carrying VP4 and VP7 gene of the bovine group A rotavirus (P[11],G8), in other one carrying a VP4 gene of bovine origin (P[1]) and a VP7 gene of swine origin (G5).
authors.affiliation: Laboratory of Animal Virology, Department of Preventive Veterinary Medicine, Londrina State University, Londrina, PR, Brazil.
authors.forename: Alice Fernandes
authors.initials: AF
authors.lastname: Alfieri
authors.forename: Amauri Alcindo
authors.initials: AA
authors.lastname: Alfieri
authors.forename: Marco Antônio Bacellar
authors.initials: MA
authors.lastname: Barreiros
authors.forename: José Paulo Gagliardi
authors.initials: JP
authors.lastname: Leite
authors.forename: Leonardo José
authors.initials: LJ
authors.lastname: Richtzenhain
mesh_terms: D000818:Animals; D001938:Brazil; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000821:virology*; D003967:Diarrhea / Q000662:veterinary / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005243:Feces / Q000821:virology; D005838:Genotype; D007124:Immunoenzyme Techniques / Q000662:veterinary; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012401:Rotavirus / Q000235:genetics*; D012400:Rotavirus Infections / Q000097:blood / Q000662:veterinary* / Q000821:virology
chemical_list: D012367:RNA, Viral