title: Skeletonization does not influence internal thoracic artery innervation.
abstract: BACKGROUND
  This study was designed to compare the effect of surgical harvesting on internal thoracic artery innervation and to assess the eventual presence of denervation supersensitivity in skeletonized grafts.
  
  
  METHODS
  Nineteen patients who underwent primary isolated coronary artery bypass grafting were randomly assigned to receive a skeletonized (n = 9) or pedicled (n = 10) internal thoracic artery graft. Immunohistochemical nerve localization using anti-S-100 protein, anti-160-kd neurofilament polypeptide and anti-tyrosine hydroxylase antibodies was performed on distal specimens of arteries to study vascular innervation. Moreover, endovascular vasoactive challenges using serotonin and methylergometrine were performed at early angiographic control to evaluate the eventual presence of denervation supersensitivity.
  
  
  RESULTS
  Quantitative analysis of immunohistochemical specimens revealed lack of difference in the number of positive cells between skeletonized and pedicled arteries for all the antibodies used. No difference in the reaction to serotonin and methylergometrine was found between skeletonized and pedicled arteries.
  
  
  CONCLUSIONS
  Skeletonization does not influence internal thoracic artery innervation.
authors.affiliation: Department of Cardiac Surgery, Institute of Human Anatomy, Catholic University, Rome, Italy. mgaudino@tiscali.it
authors.forename: Mario
authors.initials: M
authors.lastname: Gaudino
authors.forename: Amelia
authors.initials: A
authors.lastname: Toesca
authors.forename: Franco
authors.initials: F
authors.lastname: Glieca
authors.forename: Fabiana
authors.initials: F
authors.lastname: Girola
authors.forename: Nicola
authors.initials: N
authors.lastname: Luciani
authors.forename: Gianfederico
authors.initials: G
authors.lastname: Possati
mesh_terms: D000368:Aged; D017023:Coronary Angiography; D001026:Coronary Artery Bypass / Q000379:methods; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008323:Mammary Arteries / Q000737:chemistry / Q000294:innervation* / Q000637:transplantation; D008875:Middle Aged; D016900:Neurofilament Proteins / Q000032:analysis; D009418:S100 Proteins / Q000032:analysis; D020858:Tissue and Organ Harvesting / Q000379:methods*; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis; D014666:Vasomotor System / Q000187:drug effects / Q000502:physiology
chemical_list: D016900:Neurofilament Proteins; D009418:S100 Proteins; D014446:Tyrosine 3-Monooxygenase