Simultaneous determination of histamine and histidine by liquid chromatography following intramolecular excimer-forming fluorescence derivatization with pyrene-labeling reagent.
A highly sensitive and selective fluorometric method for the determination of histamine and histidine has been developed. This method is based on an intramolecular excimer-forming fluorescence derivatization with a pyrene reagent followed by reversed-phase liquid chromatography. The analytes, containing two amino moieties in a molecule, were converted to the corresponding dipyrene-labeled derivatives by derivatization. The derivatives afforded intramolecular excimer fluorescence (440 - 540 nm), which can clearly be discriminated from the normal fluorescence (360 - 420 nm) emitted from reagent blanks. The detection limits (signal-to-noise ratio = 3) were femto mole levels.
Yoshida
Hideyuki
H
Faculty of Pharmaceutical Sciences, Fukuoka University, Johnan, Fukuoka 814-0180, Japan.
Ichinose
Fumio
F
Yoshitake
Takashi
T
Nakano
Yukitaka
Y
Todoroki
Kenichiro
K
Nohta
Hitoshi
H
Yamaguchi
Masatoshi
M
D002138:Calibration; D002853:Chromatography, Liquid; D005456:Fluorescent Dyes; D006632:Histamine / Q000032:analysis*; D006639:Histidine / Q000032:analysis*; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D011721:Pyrenes / Q000737:chemistry; D012015:Reference Standards; D012996:Solutions; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D011721:Pyrenes; D012996:Solutions; D006639:Histidine; D006632:Histamine