title: An in vitro model for purging of tumour cells from ovarian tissue.
abstract: BACKGROUND
  Cryopreservation and autografting of ovarian tissue may preserve fertility after cancer treatment, but could be hampered by tumour cell contamination. Epithelial tumour cell lysis can be obtained with cytotoxic T cell retargeting through the bispecific antibody BIS-1, with combined affinity for the T-cell receptor and epithelial glycoprotein-2 (EGP-2). Our aim was to study the concept of tumour cell purging in the setting of a suspension of ovarian tissue.
  
  
  METHODS
  Human ovarian tissue was brought into suspension by mechanical and enzymatic treatment. Cells of the MCF-7 breast cancer cell line and activated human lymphocytes were co- incubated for 4 h with or without BIS-1 and with or without ovarian suspension. After incubation, MCF-7 cell death and cell growth were evaluated by fluorescent cell detection and MTT assay, respectively. Ovarian tissue morphology was evaluated immunohistochemically.
  
  
  RESULTS
  MCF-7 cell death and cell growth inhibition increased with increasing ratios of lymphocytes to MCF-7 cells. BIS-1 addition gave further augmentation, with a maximum depletion of growing MCF-7 cells of 89% (SD 11%) versus without BIS-1, 23% (SD 15%; P < 0.001). Follicles remained morphologically intact.
  
  
  CONCLUSIONS
  Purging of added epithelial tumour cells from ovarian tissue with BIS-1 is possible in vitro. Morphologically, follicles remain intact after this procedure. This method may contribute to safer replacement of ovarian tissue in female cancer survivors.
authors.affiliation: Department of Medical Oncology, University Hospital, Groningen, The Netherlands.
authors.forename: C P
authors.initials: CP
authors.lastname: Schröder
authors.forename: H
authors.initials: H
authors.lastname: Timmer-Bosscha
authors.forename: J G
authors.initials: JG
authors.lastname: Wijchman
authors.forename: L F M H
authors.initials: LF
authors.lastname: de Leij
authors.forename: H
authors.initials: H
authors.lastname: Hollema
authors.forename: M J
authors.initials: MJ
authors.lastname: Heineman
authors.forename: E G E
authors.initials: EG
authors.lastname: de Vries
mesh_terms: D000293:Adolescent; D000328:Adult; D018033:Antibodies, Bispecific; D001943:Breast Neoplasms / Q000276:immunology / Q000517:prevention & control; D016923:Cell Death; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods*; D015925:Cryopreservation; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007247:Infertility, Female / Q000628:therapy; D006080:Ovarian Follicle / Q000166:cytology* / Q000473:pathology / Q000637:transplantation*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology / Q000517:prevention & control*; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology; D014182:Transplantation, Autologous
chemical_list: D018033:Antibodies, Bispecific