RNA interference against retroviruses.
Bang and Ellerman, and later Peyton Rous, reported the first identification of transmissible cancer-causing agents, which later turned out to be avian retroviruses. Today avian retroviruses are important models for study of retrovirus replication and pathogenesis, and also important pathogens of domestic fowl. Here we describe the use of RNA interference (RNAi) in live chick embryos to block replication of an avian retrovirus. We also describe inhibition of ASLV and HIV replication in cell culture with RNAi.
Hu
Wen-Yuan
WY
Infectious Disease Laboratory, The Salk Institute, 10010 North Torrey Pines Rd., La Jolla, CA 92037, USA.
Bushman
Frederic D
FD
Siva
Amara C
AC
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000502:physiology; D002642:Chick Embryo; D018274:Electroporation; D015967:Gene Expression Regulation, Viral; D015316:Genetic Therapy / Q000379:methods; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D012190:Retroviridae / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D014779:Virus Replication
D034741:RNA, Small Interfering