A preliminary study on chitosan and gelatin polyelectrolyte complex cytocompatibility by cell cycle and apoptosis analysis.
In this study, L929 cell adhesion, proliferation and apoptosis were investigated on chitosan (CS) and chitosan-gelatin (CS-Gel) membranes having different degrees of deacetylation (DD). The mitochondrial activity [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay results demonstrated that the DD played a key role in the L929 cell adhesion. Indeed, the higher the DD of a CS membrane, the stronger the cell adhesion. The cell cycle analysis showed that the CS surface inhibits most of the cells entering the cell cycle and the DD of CS had little effect on the cell cycle progression. On the other hand, this study also confirmed that CS, blended with Gel, induced L929 cells to enter the cell cycle, encouraged proliferation, decreased the degree of apoptosis on the CS membranes, and are comparable to the results from L929 cells on tissue culture plates. In brief, CS modified with Gel improves cytocompatibility by shielding the positively charged CS with a high charge density.
Mao
Jin Shu
JS
Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, People's Republic of China.
Cui
Yuan Lu
YL
Wang
Xiang Hui
XH
Sun
Yi
Y
Yin
Yu Ji
YJ
Zhao
Hui Ming
HM
De Yao
Kang
K
D000818:Animals; D017209:Apoptosis; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D004573:Electrolytes / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D005780:Gelatin / Q000737:chemistry*; D046911:Macromolecular Substances; D008422:Materials Testing; D008567:Membranes, Artificial; D051379:Mice; D010865:Pilot Projects; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D004573:Electrolytes; D046911:Macromolecular Substances; D008567:Membranes, Artificial; D005780:Gelatin; D048271:Chitosan