[Effect of jingui shenqi pill on pituitary adrenocorticotropic hormone gene expression in shen-yang deficiency rats].
OBJECTIVE
To explore the effect of Jingui Shenqi pill (JGSQP) with various concentrations at different time points on pituitary adrencorticotropic hormone (ACTH) gene expression level in Shen-Yang deficiency rats.


METHODS
The Shen-Yang deficiency rats were randomly divided into the model control group and the high, medium and low dosage of JGSQP groups. Reverse transcriptase polymerase chain reaction was used to observe the effect of JGSQP on the ACTH mRNA of pituitary tissue in rats treated at different time points (10 d, 20 d, 30 d).


RESULTS
As compared with that in the model group, the ACTH gene expression level was significantly higher in the high dose JGSQP group (P < 0.05), and the increment in the medium dosage group was significantly higher in comparing with that in the high and low dosage groups (P < 0.05 or P < 0.01).


CONCLUSIONS
Through up-regulation on ACTH gene expression is possibly one of the mechanisms of JGSQP in treating Shen-Yang deficiency.
Zheng
Xiao-wei
XW
Zhejiang College of TCM, Hangzhou 310053.
Bao
Su-zhen
SZ
Liu
Ming-zhe
MZ
D000324:Adrenocorticotropic Hormone / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007030:Hypothalamo-Hypophyseal System; D007674:Kidney Diseases / Q000235:genetics / Q000378:metabolism; D008516:Medicine, Chinese Traditional; D010902:Pituitary Gland / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D016711:Yang Deficiency / Q000235:genetics / Q000378:metabolism*
D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; C476907:liu-wei-di-huang wan; D000324:Adrenocorticotropic Hormone