Comparative analysis using a mouse model of the immunogenicity of artificial VLP and attenuated Salmonella strain carrying a DNA-vaccine encoding HIV-1 polyepitope CTL-immunogen.
Two systems have been examined for delivery of DNA-vaccine encoding a HIV-1 polyepitope CTL-immunogen (TCI). One is intended for i.m. injection and is in the form of an artificial virus like particle containing eukaryotic expression plasmid pcDNA-TCI encapsulated within a spermidine-polyglucin conjugate. The other is intended for mucosal immunization and is based on attenuated Salmonella typhimurium strain 7207, which can deliver pcDNA-TCI directly into professional antigen-presenting cells (APC). After immunization, the artificial VLP and recombinant Salmonella induced an enhanced HIV specific serum antibody, proliferative and CTL responses compared to those induced by naked pcDNA-TCI. The most significant responses were produced when pcDNA-TCI was delivered by Salmonella.
Karpenko
Larisa I
LI
The Joint-Stock Company Vector Best, The State Research Center of Virology and Biotechnology VECTOR, Novosibirsk Region, 630559 Koltsovo, Russia. karpenko@vector.nsc.ru
Nekrasova
Nadezhda A
NA
Ilyichev
Alexander A
AA
Lebedev
Leonid R
LR
Ignatyev
George M
GM
Agafonov
Alexander P
AP
Zaitsev
Boris N
BN
Belavin
Pavel A
PA
Seregin
Sergei V
SV
Danilyuk
Nadezhda K
NK
Babkina
Irina N
IN
Bazhan
Sergei I
SI
D016915:AIDS Vaccines / Q000008:administration & dosage / Q000276:immunology*; D000285:Administration, Rectal; D000818:Animals; D002455:Cell Division / Q000502:physiology; D003470:Culture Media; D004279:DNA, Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D007273:Injections, Intramuscular; D051379:Mice; D018625:Microscopy, Atomic Force; D010957:Plasmids / Q000276:immunology; D012475:Salmonella / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology
D016915:AIDS Vaccines; D003470:Culture Media; D004279:DNA, Viral; D000939:Epitopes; D019444:Vaccines, DNA