Spectrophotometric determination of glucosamine and its analogous amino sugars with o-hydroxyhydroquinonephthalein and palladium(II).
A simple and highly sensitive spectrophotometric method for the determination of glucosamine and its analogous amino sugars was established based on fading of the palladium(II)-o-hydroxyhydroquinonephthalein-hexadecyltrimethylammonium complex. In the determination of glucosamine, Beer's law is obeyed in the range of 0.02 - 0.18 microg ml(-1), with an effective molar absorptivity at 630 nm and the relative standard deviation being 8.4 x 10(5) dm3 mol(-1) cm(-1) and 1.08% (n = 10). This method is about 70-times more sensitive than the Elson-Morgan method. The method was successfully applied to the assay of glucosamine in actual samples.
Yamaguchi
Takako
T
Osaka University of Pharmaceutical Sciences, Nasahara, Takatsuki, Osaka 569-1094, Japan.
Inoue
Mayumi
M
Miyachi
Kanako
K
Tominaga
Hiroshi
H
Fujita
Yoshikazu
Y
D000606:Amino Sugars / Q000032:analysis*; D016477:Artifacts; D002138:Calibration; D005944:Glucosamine / Q000032:analysis*; D006873:Hydroquinones / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry*
D000606:Amino Sugars; D006873:Hydroquinones; C039209:2-hydroxyhydroquinonephthalein; D010165:Palladium; D005944:Glucosamine