title: Distribution and molecular investigation of highly thermophilic bacteria associated with cool soil environments.
abstract: In this study, both molecular and culture-based methods were used to characterize thermophilic bacteria associated with the subsurface soil environment in Northern Ireland. A total of 53 thermophilic, aerobic, sporulating and non-sporulating bacteria were isolated from subsurface soil samples obtained from two sites. They were screened by amplified ribosomal DNA restriction analysis prior to 16 S rRNA gene sequencing. The majority of the sequences were associated with Geobacillus thermoleovorans (50%) and Geobacillus caldoxylosilyticus (34.6%). Isolates F10, F20 and Tf exhibited only 93% similarity with Geobacillus toebii strain F70. Hence they may represent a new species of the genus Geobacillus.
authors.affiliation: School of Biological and Environmental Sciences, University of Ulster, Coleraine, County Londonderry, Northern Ireland BT52 1SA, U.K.
authors.forename: T J
authors.initials: TJ
authors.lastname: Rahman
authors.forename: R
authors.initials: R
authors.lastname: Marchant
authors.forename: I M
authors.initials: IM
authors.lastname: Banat
mesh_terms: D001407:Bacillus / Q000235:genetics*; D001419:Bacteria / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D016680:Genome, Bacterial; D006358:Hot Temperature; D009660:Northern Ireland; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012987:Soil
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012987:Soil; D004247:DNA