[Study on repairing injured middle brain by Schwann cells transplantation].
OBJECTIVE
To explore the ability of grafted Schwann cells to promote restore of injured neurons in rat.


METHODS
Schwann cells labeled by BrdU in vitro transplanted into rat middle brain area prior to injure with electric needle stimulus. Immunohistochemistry and image analyzer were used to investigate the expression of BrdU and GAP-43 as well as quantitative analysis respectively.


RESULTS
BrdU positive cells could be identified for up to 8 months and the number increased about 15%, which mainly migrated toward injured ipsilateral cortex. The GAP-43 expression reached its peak one month after transplantation and was significant compared with control group (P < 0.05).


CONCLUSIONS
The transplantation of Schwann cells could promote the restoration of injured neurons.
Lin
Ye
Y
China-Japan Union Hospital, Jilin University, Changchun 130031, China.
Zhu
Qing-san
QS
Yin
Fei
F
Guo
Li
L
D000818:Animals; D017690:Cell Transplantation; D019922:GAP-43 Protein / Q000032:analysis; D007150:Immunohistochemistry; D008636:Mesencephalon / Q000293:injuries / Q000378:metabolism / Q000601:surgery*; D051381:Rats; D017208:Rats, Wistar; D012583:Schwann Cells / Q000378:metabolism / Q000637:transplantation*; D013997:Time Factors
D019922:GAP-43 Protein