title: Absence of Fas protein detection by flow cytometry in human spermatozoa.
abstract: OBJECTIVE
  To investigate the expression of Fas protein on the surface of ejaculated spermatozoa of normozoospermic and nonnormozoospermic men.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  University infertility clinic.
  
  
  METHODS
  Twenty-three volunteer normozoospermic men (controls) and 43 men undergoing infertility evaluation (cases).
  
  
  METHODS
  Analysis of ejaculated spermatozoa by indirect immunofluorescence of Fas protein by flow cytometry.
  
  
  METHODS
  Comparison of flow cytometric analysis of autofluorescence, control tests (secondary antibody and isotype control), and experimental tests (anti-Fas monoclonal antibody) in the spermatozoa of ejaculated samples.
  
  
  RESULTS
  No expression of Fas protein was found on the surface of ejaculated spermatozoa of controls and cases.
  
  
  CONCLUSIONS
  The Fas molecules are not present in substantial amounts in the ejaculated spermatozoa of normozoospermic and nonnormozoospermic men. Therefore, our results do not support the "abortive apoptosis" theory.
authors.affiliation: Institute of Maternal and Child Research, School of Medicine, University of Chile, San Borja-Arriarán Clinical Hospital, Santiago, Chile.
authors.forename: Andrea
authors.initials: A
authors.lastname: Castro
authors.forename: Daniela
authors.initials: D
authors.lastname: Parodi
authors.forename: Ignacio
authors.initials: I
authors.lastname: Morales
authors.forename: Marcia
authors.initials: M
authors.lastname: Madariaga
authors.forename: Rafael
authors.initials: R
authors.lastname: Rios
authors.forename: Rosita
authors.initials: R
authors.lastname: Smith
mesh_terms: D000911:Antibodies, Monoclonal; D016022:Case-Control Studies; D004542:Ejaculation; D005434:Flow Cytometry; D005453:Fluorescence; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism*; D008297:Male; D013094:Spermatozoa / Q000737:chemistry*; D014019:Tissue Donors; D019014:fas Receptor / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D019014:fas Receptor