title: Human miscarriage is associated with increased number of CD26 decidual lymphocytes.
abstract: Dipeptidyl peptidase-IV (DPP-IV, CD26), a serine protease with broad distribution in mammalian tissues and known activity in serum, participates in T-cell activation and promotes a Th1-like cytokine response. Previous data on murine abortion indicate that DPP-IV may play a critical role in pregnancy failure by inducing a Th1 local response. Here, we investigated the possible participation of DPP-IV in the onset of human spontaneous abortion (SA). The systemic (peripheral blood) and local (decidua) percentages of CD4(+), CD8(+), CD26(+) and CD56(+) cells as well as the number of Th1 lymphocytes (CCR5(+) cells) were assessed in samples from women after SAs (n = 20) and from women with normally progressing pregnancies (NPs) (n = 27) using flow cytometry and immunohistochemistry. We further measured the DPP-IV activity and concentrations of Th1 (interferon-gamma and tumour necrosis factor-alpha), Th2 [interleukin-4 (IL-4), IL-10] and Th3 (transforming growth factor-beta2) cytokines in serum samples. We could not find any difference in the number of CD4(+), CD8(+), CD26(+), CD26(+)/CD4(+) or CD8(+)/CD26(+) blood cells between NP and SA patients. No differences in the Th1, Th2 or Th3 cytokine levels could be observed between both groups. However, the percentages of decidual CD26(+) lymphocytes as well as the number of decidual Th1 cells were significantly higher in SA samples compared to samples from patients with NP. Our data support the hypothesis that CD26(+) decidual lymphocytes with DPP-IV activity may play a critical role in SAs, as previously suggested in an abortion mice model. This abortive effect may be mediated by enhancing the levels of Th1 abortogenic cytokines only locally.
authors.affiliation: Medizinische Klinik m. S. Psychosomatik, Charité, Humboldt Universität zu Berlin, Germany.
authors.forename: U S
authors.initials: US
authors.lastname: Sehmsdorf
authors.forename: A C
authors.initials: AC
authors.lastname: Zenclussen
authors.forename: P
authors.initials: P
authors.lastname: Arck
authors.forename: K
authors.initials: K
authors.lastname: Hertwig
authors.forename: R A
authors.initials: RA
authors.lastname: Joachim
authors.forename: B
authors.initials: B
authors.lastname: Klapp
authors.forename: M O
authors.initials: MO
authors.lastname: Hildebrandt
mesh_terms: D000022:Abortion, Spontaneous / Q000276:immunology*; D000293:Adolescent; D000328:Adult; D002452:Cell Count; D016207:Cytokines / Q000276:immunology; D003656:Decidua / Q000276:immunology*; D018819:Dipeptidyl Peptidase 4 / Q000097:blood / Q000276:immunology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016131:Lymphocyte Subsets / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D011247:Pregnancy
chemical_list: D016207:Cytokines; D018819:Dipeptidyl Peptidase 4