Maximizing recovery of native protein from aggregates by optimizing pressure treatment.
Recovering native protein from aggregates is a common obstacle in the production of recombinant proteins. Recent reports have shown that hydrostatic pressure is an attractive alternative to traditional denature-and-dilute techniques, both in terms of yield and process simplicity. To determine the effect of process variables, we subjected tailspike aggregates to a variety of pressure-treatment conditions. Maximum native tailspike yields were obtained with only short pressure incubations (<5 min) at 240 MPa. However, some tailspike aggregates were resistant to pressure, despite multiple cycles of pressure. Extending the postpressure incubation time to 4 days improved the yield of native protein from aggregates from 19.4 +/- 0.9 to 47.4 +/- 19.6 microg/mL (approximately 78% yield of native trimer from nonaggregate material). The nearly exclusive conversion of monomer to trimer over the time scale of days, when combined with previous kinetic data, allows for the identification of three postpressure kinetic phases: a rapid phase consisting of structured dimer conversion to trimer (30 min), an intermediate phase consisting of monomer conversion to aggregate (100 min), and a slow phase consisting of conversion of monomer to trimer (days). Optimizing the production of structured dimer can yield the highest level of folded protein. Typical refolding additives, such as glycerol, or low-temperature incubation did not improve yields.
Lefebvre
Brian G
BG
Department of Chemical Engineering, University of Delaware, Newark, Delaware 19716, USA.
Gage
Matthew J
MJ
Robinson
Anne Skaja
AS
D045424:Complex Mixtures / Q000737:chemistry / Q000302:isolation & purification; D019281:Dimerization; D005990:Glycerol / Q000737:chemistry*; D006026:Glycoside Hydrolases; D046911:Macromolecular Substances; D011312:Pressure; D011485:Protein Binding; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D013696:Temperature; D014508:Urea / Q000737:chemistry; D017376:Viral Tail Proteins / Q000737:chemistry* / Q000302:isolation & purification*
D045424:Complex Mixtures; D046911:Macromolecular Substances; D011994:Recombinant Proteins; D017376:Viral Tail Proteins; D014508:Urea; D006026:Glycoside Hydrolases; C083268:tailspike protein, bacteriophage; D005990:Glycerol