[The expression of API2-MALT1 fusion gene and its influence on the cellular apoptosis and proliferation in gastrointestinal MALT lymphoma].
OBJECTIVE
To investigate the expression of API2-MALT1 fusion gene mRNA and its effect on protein of API2, Caspase3 and Ki67 in gastrointestinal MALT lymphoma (Extranodal marginal zone B cell lymphoma of mucosa-associated lymphoid tissue).


METHODS
The mRNA of API2-MALT1 fusion transcript was assayed in 33 archival cases of gastrointestinal MALT lymphoma by RT-PCR and nested PCR. According to the expression of API2-MALT1 mRNA or not, 33 cases of MALT lymphoma were divided into two groups. Ki67, API2 and Caspase3 were detected by immunohistochemical technique and analyzed by the microsoft Image-Pro plus.


RESULTS
API2-MALT1 fusion gene mRNA was found in 16 of specimens with gastrointestinal MALT lymphoma. The expression rate was 48.4%. The average area (21177.30 +/- 6171.72) micron 2 and single cell average area (29.81 +/- 7.40) micron 2 of API2 were decreased in the group with the expression of API2-MALT1 mRNA, compared with those in the group without the expression of API2-MALT1 mRNA [(34325.65 +/- 6577.00) micron 2, (37.84 +/- 8.55) micron 2]. The integrated optical density(IOD) of API2 showed no significant difference between the two groups, neither did the expression of Ki67 and Caspase3.


CONCLUSIONS
The expression of API2-MALT1 is related to the down-regulated expression of protein of API2, its effect on apoptosis of MALT lymphoma remains to be detected further. The expression of API2-MALT1 mRNA is unrelated to the cellular proliferation.
Yang
Wen-xiu
WX
Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, China.
Li
Gan-di
GD
Zhou
Qiao
Q
Ouyang
Qin
Q
Liu
Wei-ping
WP
Zhang
Jie
J
D017209:Apoptosis / Q000235:genetics*; D001483:Base Sequence; D053148:Caspase 3; D020169:Caspases / Q000096:biosynthesis / Q000235:genetics; D002455:Cell Division; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000235:genetics* / Q000473:pathology; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D016393:Lymphoma, B-Cell / Q000235:genetics; D018442:Lymphoma, B-Cell, Marginal Zone / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics; D015514:Oncogene Proteins, Fusion / Q000096:biosynthesis / Q000235:genetics*; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured
C415943:API2-MALT1 fusion protein, human; D051034:Inhibitor of Apoptosis Proteins; D009363:Neoplasm Proteins; D015514:Oncogene Proteins, Fusion; D011506:Proteins; D012333:RNA, Messenger; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases