Renal elimination of organic anions in rats with bilateral ureteral obstruction.
Urinary tract obstruction is an important cause of acute renal failure. Several abnormalities in renal tubular function may occur in obstructive nephropathy. The tubular secretion of organic anions is an important function of the kidney that eliminates potentially toxic organic anions from the body, however, the mechanisms involved in organic anions renal elimination in rats with bilateral ureteral obstruction (BUO) have not been elucidated. In this study, it was evaluated the renal handling of p-aminohippurate (PAH) in adult male Wistar rats with BUO. A diminished renal clearance of PAH was observed in BUO rats as consequence of a diminution in the secreted load of this organic anion. The increase in the abundance of organic anions transporter 1 (OAT1) and the absence of modification in cortical renal blood flow, measured with fluorescence microspheres, do not explain the altered secretion of PAH. The diminished Na,K-ATPase activity in cortex from obstructed kidneys might condition OAT1 function. Additionally, it is also possible to conclude that in the presence of BUO, PAH clearance is not a good estimate of renal plasma flow.
Villar
Silvina R
SR
Departamento Ciencias Fisiologicas, Farmacología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, CONICET, Suipacha 531, Rosario 2000, Argentina.
Brandoni
Anabel
A
Quaglia
Nora B
NB
Torres
Adriana M
AM
D058186:Acute Kidney Injury / Q000378:metabolism*; D000818:Animals; D001783:Blood Flow Velocity; D001835:Body Weight; D007672:Kidney Cortex / Q000378:metabolism*; D008297:Male; D009929:Organ Size; D027362:Organic Anion Transport Protein 1 / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012079:Renal Circulation / Q000502:physiology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014508:Urea / Q000097:blood; D014517:Ureteral Obstruction / Q000378:metabolism*; D010130:p-Aminohippuric Acid / Q000493:pharmacokinetics
D027362:Organic Anion Transport Protein 1; C421043:Slc22a6 protein, rat; D014508:Urea; D000254:Sodium-Potassium-Exchanging ATPase; D010130:p-Aminohippuric Acid