The oligopeptide permease (Opp) of the plant pathogen Xanthomonas axonopodis pv. citri.
The oligopeptide permease (Opp), a protein-dependent ABC transporter, has been found in the genome of Xanthomonas axonopodis pv. citri ( Xac), but not in Xanthomonas campestris pv. campestris ( Xcc). Sequence analysis indicated that 4 opp genes ( oppA, oppB, oppC, oppD/F), located in a 33.8-kbp DNA fragment present only in the Xac genome, are arranged in an operon-like structure and share highest sequence similarities with Streptomyces roseofulvus orthologs. Nonetheless, analyses of the GC content, codon usage, and transposon positioning suggested that the Xac opp operon does not have an exogenous origin. The presence of a stop codon at one of the ATP-binding domains of OppD/F would render the uptake system nonfunctional, but detection of a single polycistronic mRNA and periplasmic OppA in actively growing bacteria suggests that the Opp permease is active and could contribute to the distinct nutritional requirements and host specificities of the two Xanthomonas species.
Moutran
Alexandre
A
Departmento de Microbiologia, ICBII-USP, Av. Prof. Lineu Prestes, 1374, Cidade Universitária, São Paulo, 05508-900, Brazil.
Quaggio
Ronaldo Bento
RB
Balan
Andrea
A
Ferreira
Luis Carlos de Souza
LC
Ferreira
Rita de Cássia Café
Rde C
D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D001482:Base Composition; D015152:Blotting, Northern; D015153:Blotting, Western; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D003062:Codon / Q000032:analysis / Q000235:genetics; D018388:Codon, Terminator; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D008074:Lipoproteins / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D009876:Operon; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D012329:RNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis / Q000302:isolation & purification; D013302:Streptomyces / Q000235:genetics; D014974:Xanthomonas / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development
D001426:Bacterial Proteins; D002352:Carrier Proteins; D003062:Codon; D018388:Codon, Terminator; D004251:DNA Transposable Elements; D008074:Lipoproteins; D026901:Membrane Transport Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; C046506:oligopeptide-binding protein, bacteria; C037230:oligopeptide permease, Bacteria