title: [A plate method to screen for microorganisms producing xylose isomerase].
abstract: A plate method was developed to screen for xylose isomerase-producing microorganisms based on the use of 2,3,5-triphenyltetrazolium as an indicator of D-xylulose, the D-xylose isomerization product. The use of this method allows microorganisms to be differentiated by the character of the enzyme synthesis (inducible or constitutive).
authors.affiliation: Institute of Microbiology, National Academy of Sciences of Belarus, Minsk, 220141 Belarus. enzyme@mbio.bas-net.by
authors.forename: L I
authors.initials: LI
authors.lastname: Sapunova
authors.forename: A G
authors.initials: AG
authors.lastname: Lobanok
authors.forename: I O
authors.initials: IO
authors.lastname: Kazakevich
authors.forename: A N
authors.initials: AN
authors.lastname: Evtushenkov
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000032:analysis* / Q000096:biosynthesis; D001173:Arthrobacter / Q000302:isolation & purification* / Q000378:metabolism; D015169:Colony Count, Microbial / Q000379:methods; D004396:Coloring Agents; D013778:Tetrazolium Salts
chemical_list: D004396:Coloring Agents; D013778:Tetrazolium Salts; C009591:triphenyltetrazolium; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase