Adipose tissue-derived stromal cells express neuronal phenotypes.
BACKGROUND
Adipose tissue-derived stromal cells (ADSCs) can be greatly expanded in vitro, and induced to differentiate into multiple mesenchymal cell types, including osteogenic, chondrogenic, myogenic, and adipogenic cells. This study was designed to investigate the possibility of ADSCs differentiating into neurons.


METHODS
Adipose tissue from rats was digested with collagenase, and adherent stromal cells were cultured. A medium containing a low concentration of fetal bovine serum was adopted to induce the cells to differentiate. ADSCs were identified by immunocytochemistry, and semi-quantitative RT-PCR was applied to detect mRNA expression of neurofilament 1 (NF1), nestin, and neuron-specific enolase (NSE).


RESULTS
Nestin-positive cells were found occasionally among ADSCs. ADSCs were found to express NSE mRNA and nestin mRNA, but not NF1 mRNA. ADSCs could differentiate into neuron-like cells in a medium composed of a low concentration of fetal bovine serum, and these differentiated cells displayed complicated neuron-like morphologies.


CONCLUSIONS
The data support the hypothesis that adipose tissue contains stem cells capable of differentiating into neurons. These stem cells can overcome their mesenchymal commitment, and may represent an alternative autologous stem cell source for CNS cell transplantation.
Yang
Li-ye
LY
Department of Neurosurgery, First Affiliated Hospital, Suzhou University, Suzhou 215006, China. yangleeyee@sina.com
Liu
Xiang-ming
XM
Sun
Bing
B
Hui
Guo-zhen
GZ
Fei
Jian
J
Guo
Li-he
LH
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis; D009419:Nerve Tissue Proteins; D064231:Nestin; D016900:Neurofilament Proteins / Q000032:analysis; D009474:Neurons / Q000166:cytology*; D010641:Phenotype; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D051381:Rats
D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D016900:Neurofilament Proteins; D010751:Phosphopyruvate Hydratase