[Connectivity between soxS gene expression and adaptive resistance to the SoxRS-regulon inducers in E. coli cells].
Development of the adaptive response (AR) to the SoxRS-inducers-menadione (O2(-.)-donor), dinitrosyl-iron complex (NO donor) and their simultaneous action was studied in E. coli. Two AR parameters were used: an increasing in viability and decreasing in the soxS gene (SoxRS-regulon) expression in adapted cells. It was shown that namely peroxynitrite (ONOO-), being formed inside the cells from O2-. and NO, was the most cytotoxic agent among the drugs tested. On the one side, an increase in resistance to menadione treatment was selectively demonstrated in adapted E. coli delta oxyR mutant cells, defective in OxyR-regulon activity. On the other side, a decrease in soxS gene expression was marked in the experiments with menadione, as well So, an AR to O2-. superoxide anion was selectively regulated by the SoxRS DNA-repair pathway. OxyR-regulon that is selectively activated by the most redox-cycling agents and controls AR to these agents doesn't provide development of the AR to O2-..
Vasil'eva
S V
SV
Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, 117334 Russia.
Makhova
E V
EV
Moshkovskaia
E Iu
EIu
D004260:DNA Repair; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D008962:Models, Theoretical; D009154:Mutation; D009569:Nitric Oxide / Q000494:pharmacology; D030421:Peroxynitrous Acid / Q000494:pharmacology; D018085:Regulon; D015534:Trans-Activators / Q000235:genetics*; D024483:Vitamin K 3 / Q000494:pharmacology
D029968:Escherichia coli Proteins; D015534:Trans-Activators; C070725:SoxS protein, E coli; D030421:Peroxynitrous Acid; D009569:Nitric Oxide; D024483:Vitamin K 3