title: Comparative analysis using a mouse model of the immunogenicity of artificial VLP and attenuated Salmonella strain carrying a DNA-vaccine encoding HIV-1 polyepitope CTL-immunogen.
abstract: Two systems have been examined for delivery of DNA-vaccine encoding a HIV-1 polyepitope CTL-immunogen (TCI). One is intended for i.m. injection and is in the form of an artificial virus like particle containing eukaryotic expression plasmid pcDNA-TCI encapsulated within a spermidine-polyglucin conjugate. The other is intended for mucosal immunization and is based on attenuated Salmonella typhimurium strain 7207, which can deliver pcDNA-TCI directly into professional antigen-presenting cells (APC). After immunization, the artificial VLP and recombinant Salmonella induced an enhanced HIV specific serum antibody, proliferative and CTL responses compared to those induced by naked pcDNA-TCI. The most significant responses were produced when pcDNA-TCI was delivered by Salmonella.
authors.affiliation: The Joint-Stock Company Vector Best, The State Research Center of Virology and Biotechnology VECTOR, Novosibirsk Region, 630559 Koltsovo, Russia. karpenko@vector.nsc.ru
authors.forename: Larisa I
authors.initials: LI
authors.lastname: Karpenko
authors.forename: Nadezhda A
authors.initials: NA
authors.lastname: Nekrasova
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Ilyichev
authors.forename: Leonid R
authors.initials: LR
authors.lastname: Lebedev
authors.forename: George M
authors.initials: GM
authors.lastname: Ignatyev
authors.forename: Alexander P
authors.initials: AP
authors.lastname: Agafonov
authors.forename: Boris N
authors.initials: BN
authors.lastname: Zaitsev
authors.forename: Pavel A
authors.initials: PA
authors.lastname: Belavin
authors.forename: Sergei V
authors.initials: SV
authors.lastname: Seregin
authors.forename: Nadezhda K
authors.initials: NK
authors.lastname: Danilyuk
authors.forename: Irina N
authors.initials: IN
authors.lastname: Babkina
authors.forename: Sergei I
authors.initials: SI
authors.lastname: Bazhan
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage / Q000276:immunology*; D000285:Administration, Rectal; D000818:Animals; D002455:Cell Division / Q000502:physiology; D003470:Culture Media; D004279:DNA, Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D007273:Injections, Intramuscular; D051379:Mice; D018625:Microscopy, Atomic Force; D010957:Plasmids / Q000276:immunology; D012475:Salmonella / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology
chemical_list: D016915:AIDS Vaccines; D003470:Culture Media; D004279:DNA, Viral; D000939:Epitopes; D019444:Vaccines, DNA