Structure and fungicidal activity of a synthetic antimicrobial peptide, P18, and its truncated peptides.
P18 (KWKLFKKIPKFLHLAKKF-NH2) is an antimicrobial peptide designed from a cecropin A-magainin 2 hybrid that has potent antibacterial activity without hemolytic activity against human erythrocytes. In this study, P18 displayed potent fungicidal activity (MIC: 12.5 approximately 25 microM) against pathogenic fungi, Candida albicans, Trichosporon beigelii, Aspergillus flavus and Fusarium oxyspovrum. The central Pro9 residue and the entire sequence of P18 are essential for its full fungicidal activity. Circular dichroism analysis suggested that the higher alpha-helical content of the peptides did not correlate with the stronger fungicidal activity.
Lee
Dong Gun
DG
School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Puk-ku, Taegu 702-701, Korea.
Hahm
Kyung-Soo
KS
Shin
Song Yub
SY
D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000494:pharmacology*; D001230:Aspergillus / Q000378:metabolism; D002176:Candida albicans / Q000737:chemistry; D002942:Circular Dichroism; D005670:Fusarium / Q000378:metabolism; D017353:Gene Deletion; D007930:Leucine / Q000737:chemistry; D054806:Magainins; D010455:Peptides / Q000737:chemistry; D011392:Proline / Q000737:chemistry; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D013329:Structure-Activity Relationship; D013778:Tetrazolium Salts / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry; D014250:Trichosporon / Q000378:metabolism; D014981:Xenopus; D029867:Xenopus Proteins / Q000737:chemistry
D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D054806:Magainins; C447608:P18 antimicrobial peptide; D010455:Peptides; D013778:Tetrazolium Salts; D013844:Thiazoles; D029867:Xenopus Proteins; C053301:magainin 2 peptide, Xenopus; C031161:cecropin A; D011392:Proline; C022616:thiazolyl blue; D007930:Leucine