[Effect of fragile histidine triad gene on apoptosis of MGC-803 gastric cancer cells].
To investigate the effect of fragile histidine triad (FHIT) gene on the apoptosis of gastric cancer cells.



FHIT gene was transfected into gastric cancer cell line MGC-803 by liposome. Western blot and immunohistochemical assay were employed to detect the expression of FHIT. Apoptosis and cell cycle were analyzed by flow cytometry (FCM). Morphological changes were observed by light and electron microscope.



Stable expression of FHIT protein in transfected MGC-803 cells was obtained. The rates of G0/G1 phase and apoptosis cells were significantly higher in FHIT-transfected MGC-803 cells than those in control cells (64.4%compared with 49.5%and 32.0%compared with 12.2%, respectively).



The results indicate that FHIT gene might be involved in the apoptosis of gastric cancer cells.
Kong
Mei
M
College of Medicine, Zhejiang University, Hangzhou 310031, China.
Chen
Wei-zhe
WZ
Chen
Zhi
Z
Li
Min-wei
MW
D017766:Acid Anhydride Hydrolases; D017209:Apoptosis; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D016147:Genes, Tumor Suppressor; D006801:Humans; D007150:Immunohistochemistry; D009363:Neoplasm Proteins / Q000032:analysis / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology* / Q000517:prevention & control; D014162:Transfection; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase