title: Simple Algorithm for Identification of Bordetella pertussis Pertactin Gene Variants.
abstract: Studies performed in several countries have demonstrated the recent emergence and subsequent dominance of circulating Bordetella pertussis strains harboring pertactin and pertussis toxin variants not included in pertussis vaccines. Determination of the pertactin gene variants is commonly performed using a time-consuming and expensive sequence analysis. We developed a simple and reliable pertactin typing algorithm suitable for large-scale screening. The assay correctly identified all pertactin alleles in representative strains. The typing of 231 clinical strains of B. pertussis routinely isolated in Belgium showed that this algorithm was adequate to identify less-frequent prn types like prn9 and prn11.
authors.affiliation: Belgian Reference Laboratory for Pertussis, Department of Microbiology, Academisch Ziekenhuis Vrije Universiteit Brussel, Brussels, Belgium.
authors.forename: Gaëtan
authors.initials: G
authors.lastname: Muyldermans
authors.forename: Denis
authors.initials: D
authors.lastname: Piérard
authors.forename: Nathalie
authors.initials: N
authors.lastname: Hoebrekx
authors.forename: Reza
authors.initials: R
authors.lastname: Advani
authors.forename: Shirley
authors.initials: S
authors.lastname: Van Amersfoorth
authors.forename: Iris
authors.initials: I
authors.lastname: De Schutter
authors.forename: Oriane
authors.initials: O
authors.lastname: Soetens
authors.forename: Leo
authors.initials: L
authors.lastname: Eeckhout
authors.forename: Anne
authors.initials: A
authors.lastname: Malfroot
authors.forename: Sabine
authors.initials: S
authors.lastname: Lauwers
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D001886:Bordetella pertussis / Q000145:classification* / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D010566:Virulence Factors, Bordetella / Q000235:genetics*; D014917:Whooping Cough / Q000382:microbiology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D010566:Virulence Factors, Bordetella; C068929:pertactin