title: Rapid method for evaluating reversed-phase high-performance liquid chromatography column stability.
abstract: A procedure is presented for the rapid evaluation of HPLC stationary phase stability at pH 8.4 or 10.1 using a temperature of 60 degrees C. Mobile phase (MeOH-0.1 mol l(-1) aqueous NaHCO3, 50:50, v/v) is continuously passed through the column with periodic injections of a test solution until the several chromatographic parameters of the resulting chromatograms are degraded. The tests were applied to several commercial and laboratory-made stationary phases. After degradation two of these phases, one commercial and one laboratory-made, were examined by elemental analysis and scanning electron microscopy to elucidate the degradation process.
authors.affiliation: Instituto de Química, Universidade Estadual de Campinas, Caixa Postal 6154, 13084-971 Campinas, SP, Brazil.
authors.forename: Dania A
authors.initials: DA
authors.lastname: Fonseca
authors.forename: Humberto R
authors.initials: HR
authors.lastname: Gutiérrez
authors.forename: Kenneth E
authors.initials: KE
authors.lastname: Collins
authors.forename: Carol H
authors.initials: CH
authors.lastname: Collins
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D006863:Hydrogen-Ion Concentration