Intra-strain dioxin sensitivity and morphometric effects in swim-up rainbow trout (Oncorhynchus mykiss).
Inter and intra-specific differences in sensitivity of early life stage salmonids to 2,3,7,8-TCDD exposure have been reported, but intra-strain differences have not been found in the literature. Our results indicate that intra-strain variability in terms of embryo mortality (LD50) is small in Eagle Lake strain of rainbow trout, LD50 values ranging from 285 to 457 pg TCDD egg g(-1). These results confirm Eagle Lake as a less sensitive strain within rainbow trout, and do not indicate overlap with reported LD50 values for brook or lake trout. Our results also demonstrate that although generalized edema in regions including the yolk-sac are frequently associated with mortality following dioxin exposure, not all edematous fish die. We detected dose-dependent decreases in cranial length, eye diameter, mass, and total length (P < 0.05) in viable swim-up rainbow trout. These effects are presumed to indicate more subtle dose-dependent disruptions of the viteline vein vasculature and, therefore, in access to energy sources. A tendency for dose-dependent decrease in liver glycogen reserves concurred with previous results on salmonids and with the well described TCDD-induced alterations in intermediate metabolism of rats and chicken embryos (wasting syndrome). This syndrome could be contributing to the reduced growth that we observed.
Carvalho
Paulo S M
PS
University of Missouri-Columbia, Fisheries and Wildlife Department, 302 Anheuser-Busch-Columbia, Columbia, MO 65211, USA. pcarvalho@ccb.ufsc.br
Noltie
Douglas B
DB
Tillitt
Donald E
DE
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000401:mortality; D005260:Female; D007928:Lethal Dose 50; D008112:Liver Glycogen / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000033:anatomy & histology* / Q000196:embryology* / Q000254:growth & development; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology*; D013045:Species Specificity; D013550:Swimming / Q000502:physiology
D008112:Liver Glycogen; D000072317:Polychlorinated Dibenzodioxins