Detection of CD3epsilon polymorphism in cynomolgus monkeys by a method based on RFLP.
We previously reported that peripheral lymphocytes from about 12% of cynomolgus monkeys lacked reactivity with anti-rhesus monkey CD3 monoclonal antibody (FN18). The nucleotide sequence analysis of the genes encoding CD3 component proteins revealed that a single amino acid substitutions found in the CD3epsilon chain determined the phenotype. In this study, we attempted to develop a method based on the restriction fragment length polymorphism (RFLP) and apply it for determination of the genotypes of individual monkeys. Comparison of the phenotype determined by fluorescence-activated cell sorter analysis with the genotype determined by RFLP analysis revealed that the FN18 -positive trait was dominant over the FN18-negative trait. It was also revealed that allele frequency was significantly different among macaques depending on the geographical region where their ancestors were derived from.
Uda
Akihiko
A
Tsukuba Primate Center for Medical Science, National Institute of Infectious Diseases, Ibaraki, Japan.
Tanabayashi
Kiyoshi
K
Mukai
Ryozaburo
R
Terao
Keiji
K
Yamada
Akio
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D017252:CD3 Complex / Q000235:genetics*; D017931:DNA Primers; D005453:Fluorescence; D005787:Gene Frequency; D005843:Geography; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008252:Macaca fascicularis / Q000235:genetics* / Q000276:immunology; D010375:Pedigree; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000235:genetics
D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D017931:DNA Primers; D012333:RNA, Messenger