[Pulmonary artery perfusion with hypothermic solution inhibits the apoptosis of lung parenchymal cells during cardiopulmonary bypass].
OBJECTIVE
To study effects of pulmonary artery perfusion with hypothermic solution on the apoptosis of lung parenchymal cells during cardiopulmonary bypass.


METHODS
Forty children with tetralogy of Fallot were divided into control group (n = 20) and protective group (n = 20). The patients in control group were performed using routine approaches. Patients' pulmonary artery were infused with 4 degrees C protective solution during cardiopulmonary bypass in protective group. Lung biopsy specimens were obtained after operations in order to study the apoptosis of lung parenchymal cells using tunnel techniques. At same time, patients' pulmonary functions and clinic index were monitored.


RESULTS
The rate of apoptosis cells of lung parenchymal cells was (18 +/- 7)% in control group, whereas (10 +/- 2)% in protective group. There was significant difference between both groups (t = -2.95, P < 0.05). Index O(2) in protective group was higher than that in control group at 0, 6 and 12 hours after operations [(492 +/- 172), (444 +/- 104), (489 +/- 58) mm Hg versus (369 +/- 126), (347 +/- 107), (340 +/- 119) mm Hg, t = 2.59, P < 0.05; t = 2.88, P < 0.01; t = 5.06, P < 0.01, respectively)]. The time of mechanical ventilation was significantly shorter in protective group than in control group [(15 +/- 11) hours versus (26 +/- 15) hours, t = -2.76, P < 0.01].


CONCLUSIONS
Pulmonary artery perfusion with hypothermic solution can inhibit the apoptosis of lung parenchymal cells and relieve cardiopulmonary bypass-induced lung injury.
Wei
Bo
B
Congenital Heart Disease Center, Fuwai Heart Disease Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100037, China.
Liu
Ying-long
YL
Yu
Cun-tao
CT
Chang
Yong-nan
YN
Li
Chun-hua
CH
D017209:Apoptosis; D002315:Cardiopulmonary Bypass; D016022:Case-Control Studies; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007036:Hypothermia, Induced / Q000379:methods*; D007223:Infant; D008168:Lung / Q000098:blood supply / Q000473:pathology*; D008297:Male; D010477:Perfusion; D011651:Pulmonary Artery; D013771:Tetralogy of Fallot / Q000601:surgery