title: Immunohistochemistry used as a screening method for persistent bovine viral diarrhea virus infection.
abstract: Cattle persistently infected (PI) with bovine viral diarrhea virus(BVDV) are a major source of infection to herds. To successfully control BVDV, it is necessary to identify and cull those cattle PI with BVDV. Immunohistochemistry (IHC) is a useful tool for sensitive and specific detection of BVDV antigens in infected cattle. Skin of cattle PI with BVDV is one of the tissues where BVDV can be consistently identified by IHC and is readily accessible for sampling. Use of IHC on skin biopsies (in the form of ear notches)as a method to identify cattle PI with BVDV has resulted in a reliable, affordable technique for mass testing of cattle at an early age without maternal antibody interference. The ability to test large numbers of cattle to identify those Pl with BVDV will enable implementation of programs for control and eventual eradication of BVDV.
authors.affiliation: Nebraska Veterinary Diagnostic Center, Department of Veterinary and Biomedical Sciences, Institute of Agriculture and Natural Resources, University of Nebraska-Lincoln, Fair Street & East Campus Loop, Lincoln, NE 68583-0907, USA. bbrodersen1@unl.edu
authors.forename: Bruce W
authors.initials: BW
authors.lastname: Brodersen
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001706:Biopsy / Q000662:veterinary; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000175:diagnosis*; D002417:Cattle; D003937:Diagnosis, Differential; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology / Q000302:isolation & purification*; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary*; D008403:Mass Screening / Q000379:methods / Q000662:veterinary*; D012867:Skin / Q000821:virology
chemical_list: D000956:Antigens, Viral