Genetic organization of the basic replicon of plasmid pMTH4 of a facultatively methylotrophic bacterium Paracoccus methylutens DM12.
Two functional regions within the basic replicon of plasmid pMTH4 of Paracoccus methylutens DM12 have been distinguished that are responsible for the replication of the plasmid (REP) and its stabilization (STA). In the REP region, a gene encoding the putative replication initiation protein RepA has been identified, with the highest similarity to the replication protein of plasmid pALC1 (Paracoccus alcaliphilus). The potential origin of replication (oriV), consisting of five long repeated sequences (iterons) as well as putative DnaA and IHF boxes, has been localized in the promoter region of the gene repA. The STA region was found to ensure stability for heterogeneous plasmid pABW3 that is unstable itself in paracocci. The mini-STA region (850 bp) contains two short open reading frames, one of which shows similarity to the RelB protein of Escherichia coli. Our investigations suggest that the stabilizing system of pMTH4 is based on the toxin and antidote principle.
Szymanik
Michal
M
Institute of Microbiology, Department of Bacterial Genetics, Warsaw University, Miecznikowa 1, 02-096 Warsaw, Poland. mszym@biol.uw.edu.pl
Bartosik
Dariusz
D
Wlodarczyk
Miroslawa
M
D000595:Amino Acid Sequence; D004269:DNA, Bacterial / Q000737:chemistry; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010230:Paracoccus / Q000235:genetics*; D010957:Plasmids; D012093:Replicon
D004269:DNA, Bacterial