title: Molecular characterization of a Rhodotorula-lytic enzyme from Paecilomyces lilacinus having beta-1,3-mannanase activity.
abstract: We cloned the gene and corresponding cDNA for an extracellular Rhodotorula-lytic enzyme which has beta-1,3-mannase activity, tentatively named MAN5C, from Paecilomyces lilacinus. MAN5C showed a high homology score with the members of glycoside hydrolase family 5 in a domain search with the Pfam database, indicating that MAN5C is a novel and unique member of glycoside hydrolase family 5.
authors.affiliation: Department of Applied Life Science, Faculty of Engineering, Sojo University. Ikeda, Kumamoto, Japan. hsugino@life.sojo-u.ac.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sugino
authors.forename: Shinya
authors.initials: S
authors.lastname: Furuichi
authors.forename: Sawao
authors.initials: S
authors.lastname: Murao
authors.forename: Motoo
authors.initials: M
authors.lastname: Arai
authors.forename: Takao
authors.initials: T
authors.lastname: Fujii
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D008351:Mannans / Q000378:metabolism; D008969:Molecular Sequence Data; D010143:Paecilomyces / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D012248:Rhodotorula / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D044902:beta-Mannosidase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D008351:Mannans; D044902:beta-Mannosidase