Long-term potentiation induces expanded movement representations and dendritic hypertrophy in layer V of rat sensorimotor neocortex.
While long-term potentiation (LTP) is currently the most widely investigated model of the synaptic mechanisms underlying learning, there is a paucity of reports on the direct effects of LTP on cortical organization. Here we show that strengthening polysynaptic potentiation correlates with an expanded neocortical area that responds to intracortical microstimulation-induced movements of rat forelimb and increased dendritic material in layer V pyramidal cells. Rats carried a stimulating electrode in the corpus callosum (midline), and a recording electrode in the right caudal forelimb area (CFA). Each rat received 15 days of either high frequency stimulation (HFS) or handling. Evoked potentials of the transcallosal pathway were recorded in the right hemisphere before and after 15 days of stimulation or handling. Following the last stimulation, movement representations were determined in the left CFA using high-resolution intracortical microstimulation (ICMS) and then the brains were processed for Golgi-Cox staining. Our results show that synaptic modification results in a recruitment of more neocortical area into movement representations and increases in several measures of dendritic morphology in layers III and V. This study sheds light on the interaction between artificial models of learning, receptive field characteristics and dendritic morphology in the sensorimotor cortex.
Monfils
Marie-H
MH
Department of Psychology, University of Calgary, Calgary, Alberta, Canada T2N 1N4.
VandenBerg
Penny M
PM
Kleim
Jeffrey A
JA
Teskey
G Campbell
GC
D000222:Adaptation, Physiological / Q000502:physiology; D000818:Animals; D003712:Dendrites / Q000473:pathology / Q000502:physiology / Q000648:ultrastructure*; D004558:Electric Stimulation; D019054:Evoked Potentials, Motor / Q000502:physiology*; D005552:Forelimb / Q000166:cytology / Q000502:physiology; D017774:Long-Term Potentiation / Q000502:physiology*; D008297:Male; D009044:Motor Cortex / Q000166:cytology* / Q000502:physiology*; D009068:Movement / Q000502:physiology*; D019579:Neocortex / Q000166:cytology* / Q000502:physiology*; D009415:Nerve Net / Q000166:cytology / Q000502:physiology; D051381:Rats; D020318:Rats, Long-Evans; D013003:Somatosensory Cortex / Q000166:cytology / Q000502:physiology