Design, construction, characterization, and application of a hyperspectral microarray scanner.
We describe the design, construction, and operation of a hyperspectral microarray scanner for functional genomic research. The hyperspectral instrument operates with spatial resolutions ranging from 3 to 30 microm and records the emission spectrum between 490 and 900 nm with a spectral resolution of 3 nm for each pixel of the microarray. This spectral information, when coupled with multivariate data analysis techniques, allows for identification and elimination of unwanted artifacts and greatly improves the accuracy of microarray experiments. Microarray results presented in this study clearly demonstrate the separation of fluorescent label emission from the spectrally overlapping emission due to the underlying glass substrate. We also demonstrate separation of the emission due to green fluorescent protein expressed by yeast cells from the spectrally overlapping autofluorescence of the yeast cells and the growth media.
Sinclair
Michael B
MB
Sandia National Laboratories, Albuquerque, New Mexico 87185-1405, USA. mbsincl@sandia.gov
Timlin
Jerilyn A
JA
Haaland
David M
DM
Werner-Washburne
Margaret
M
D004867:Equipment Design; D016678:Genome; D049452:Green Fluorescent Proteins; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D055095:Optics and Photonics / Q000295:instrumentation*; D015003:Yeasts / Q000166:cytology / Q000235:genetics
D007202:Indicators and Reagents; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins