Acacia senegal and Prosopis chilensis-nodulating rhizobia Sinorhizobium arboris HAMBI 2361 and S. kostiense HAMBI 2362 produce tetra- and pentameric LCOs that are N-methylated, O-6-carbamoylated and partially sulfated.
Sinorhizobium arboris and S. kostiense are rhizobia that nodulate the tropical leguminous trees Acacia senegal and Prosopis chilensis. The lipochito-oligosaccharidic signalling molecules (LCOs) of S. arboris HAMBI 2361 and S. kostiense HAMBI 2362 were analyzed by mass spectrometry. The major LCOs produced by the strains were shown to be pentameric, acylated with common fatty acids, N-methylated, O-6-carbamoylated and partially sulfated, as are the LCOs characterized to date for other Acacia-nodulating rhizobia. Besides the major LCOs the two strains produced (i) tetrameric LCOs, (ii) LCOs acylated with fatty acids other than those commonly found, (iii) LCOs with only an acyl substituent and (iv) noncarbamoylated LCOs. Production of LCOs (i) to (iii) are novel among Acacia-nodulating rhizobia. The roles of the different structural characteristics of LCOs in the rhizobium-A. senegal symbiosis are discussed. Specific structural features of the LCOs are proposed to be important in the selection of effective nitrogen-fixing rhizobia by A. senegal.
Nowak
Petri
P
Department of Applied Chemistry and Microbiology, PO Box 56, 00014 University of Helsinki, Helsinki, Finland. petri.nowak@helsinki.fi
Soupas
Laura
L
Thomas-Oates
Jane
J
Lindström
Kristina
K
D000045:Acacia / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002853:Chromatography, Liquid; D008040:Genetic Linkage; D007477:Ions; D013058:Mass Spectrometry; D008745:Methylation; D008956:Models, Chemical; D009005:Monosaccharides / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D029862:Prosopis / Q000378:metabolism*; D012231:Rhizobium / Q000378:metabolism; D020579:Sinorhizobium / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D013455:Sulfur / Q000737:chemistry*
D007477:Ions; D009005:Monosaccharides; D009844:Oligosaccharides; D013455:Sulfur