Two sequences encoding chalcone synthase in yellow lupin (Lupinus luteus l.) may have evolved by gene duplication.
Two full copy cDNA sequences encoding chalcone synthase (CHS) were selected from a yellow lupin (Lupinus luteus L.) root and nodule cDNA library, and sequenced. Analysis of their open reading frames gave evidence that both encode the functional enzyme. Sequence alignment and phylogenetic studies on the DNA and protein level of these clones compared to the sequences of chalcone synthases from 54 other plant species reveal the possibility that lupin chalcone synthase is encoded by a multigene family consisting of at least two distinct genes that probably diverged by gene duplication. The duplication event is estimated to have taken place about 16 million years ago.
Narozna
Dorota
D
Department of Biochemistry and Biotechnology, August Cieszkowski Agricultural University of Poznań, Wołyńska 35, 60-637 Poznań, Poland.
Paś
Jakub
J
Schneider
Jolanta
J
Madrzak
Cezary J
CJ
D000217:Acyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D020440:Gene Duplication; D015723:Gene Library; D017343:Genes, Plant; D031297:Lupinus / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny
D000217:Acyltransferases; C020141:flavanone synthetase