Comparison of manual and automated methods for determining platelet counts in dogs with macrothrombocytopenia.
Platelet counts were performed in 43 Cavalier King Charles Spaniels (CKCS, a breed predisposed to macrothrombocytopenia) and in 10 control dogs using 3 automated systems and 3 manual methods (erythrocyte-lysing agents + counting chamber or evaluation of blood smear). Good correlations were found between platelet counts using all methods (all P < 0.0001; R2 = 0.71-0.85). Best correlations were found between the manual methods. Significantly larger platelets were found in CKCS with platelet count < or = 100,000/microl when compared with control dogs and CKCS with platelet count > 100,000/microl (both P < 0.0001). All platelet counts--except when made with the 2 counting chamber methods--were underestimated at platelet counts < or = 100,000/microl.
Olsen
Lisbeth H
LH
Department of Anatomy and Physiology, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Kristensen
Annemarie T
AT
Qvortrup
Karen
K
Pedersen
Henrik D
HD
D000818:Animals; D004283:Dog Diseases / Q000097:blood*; D004285:Dogs; D005260:Female; D008297:Male; D010976:Platelet Count / Q000379:methods / Q000662:veterinary*; D018709:Statistics, Nonparametric; D013921:Thrombocytopenia / Q000097:blood / Q000662:veterinary*