title: Generation, purification and functional characterization of three C3a anaphylatoxins in rainbow trout: role in leukocyte chemotaxis and respiratory burst.
abstract: Activation of the complement system leads to cleavage of the C3 molecule into C3b and C3a fragments. The C3a fragment plays a major role in immunity by inducing chemotaxis of eosinophils and mast cells and stimulating the respiratory burst in leukocytes. Although this anaphylotoxin has been well studied in mammals, there is currently a lack of information about the structure and function of C3a anaphylotoxins in non-mammalian vertebrate species. Therefore, in the present study, we have isolated and characterized three different C3a anaphylatoxin molecules from rainbow trout, a teleost fish. C3a was generated from the trout C3-1, C3-3, and C3-4 isoforms by incubating each individual C3 molecule with purified trout factor B/C2 and factor D in the presence of Mg2+, then purifying the resulting C3a molecules by gel filtration. SDS-PAGE, N-terminal sequence and mass spectrometric analysis demonstrated the high degree of purity and expected molecular masses of the three C3a molecules. We showed that although activated trout serum was able to induce complement-dependent chemotaxis in trout head kidney leukocytes, none of the three isolated C3a molecules induced chemotaxis in the same cells. In contrast, all three C3a molecules strongly stimulated the respiratory burst of head kidney leukocytes in a dose-dependent manner. When the carboxy-terminal Arg was removed from all three C3a molecules, their ability to induce the respiratory burst was lost. These studies, therefore, provide strong evidence for the existence of three functional C3a molecules in a non-mammalian vertebrate species and suggest that some of the basic mechanisms of action of the C3a molecule have been conserved for more than 300 million years.
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 413 Rosenthal, 3800 Spruce St, Philadelphia, PA 19104, USA.
authors.forename: J
authors.initials: J
authors.lastname: Rotllant
authors.forename: D
authors.initials: D
authors.lastname: Parra
authors.forename: R
authors.initials: R
authors.lastname: Peters
authors.forename: H
authors.initials: H
authors.lastname: Boshra
authors.forename: J O
authors.initials: JO
authors.lastname: Sunyer
mesh_terms: D000706:Anaphylatoxins / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D003176:Complement C3 / Q000737:chemistry / Q000276:immunology*; D015926:Complement C3a / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D011415:Complement Factor B / Q000276:immunology; D007962:Leukocytes / Q000276:immunology; D008274:Magnesium / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D017686:Oncorhynchus mykiss; D016897:Respiratory Burst / Q000276:immunology*
chemical_list: D000706:Anaphylatoxins; D003176:Complement C3; D015926:Complement C3a; D011415:Complement Factor B; D008274:Magnesium