Separation of free iodide from other I-species in human serum - Quantification in serum pools and individual samples.
A method for the determination of free iodide in human serum has been developed. Iodide from pooled serum samples has been separated from the organic matter by SEC, subsequently freeze-dried and analyzed by ion chromatography. Investigations for recovery and precision have been carried out and provided sufficient results. For quality assurance ICP-MS has been taken additionally as a total I-detector. The iodide results of ICP-MS agree well with IC values. Iodine containing SEC- fractions from iodide-spiked samples has shown no increased I-values except that in the iodide fractions, proving that there has been no iodide conversion into other I-species (and vice versa) during the whole procedure. Free iodide from two serum pools of different healthy persons has been determined as 2.25 and 2.43 microg I(-)/l, respectively. The values are related to total iodine levels determined by ICP-MS. For comparative reasons a table of individual iodine and iodide values is presented.
Michalke
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B
GSF-Forschungszentrum für Umwelt und Gesundheit, Institut für Okologische Chemie, Neuherberg, D-85764, Oberschleissheim, Germany.
Schramel
P
P
Hasse
S
S