Inhibition of vancomycin-resistant enterococcus by continuous-flow cultures of human stool microflora with and without anaerobic gas supplementation.
A continuous-flow competitive exclusion (CFCE) culture model of human stool microflora was used to examine whether supplemental anaerobic gas is necessary for maintenance of anaerobes and inhibition of vancomycin-resistant Enterococcus (VRE). CFCE cultures of human stool microflora were maintained with supplemental nitrogen, without supplemental nitrogen, or with percolated room air. Cultures with or without supplemental nitrogen maintained >9 log(10) CFU mL(-1) of obligate anaerobes and eliminated 10(6) CFU mL(-1) of VRE. When room air was percolated into the culture, anaerobes were detected at < or =2 log(10) CFU mL(-1), and the same VRE inoculum was not eliminated ( P < 0.001). These data demonstrate that human stool CFCE cultures maintain high levels of obligate anaerobes and inhibit VRE without the addition of supplemental anaerobic gas.
Hume
Michael E
ME
United States Department of Agriculture, Agricultural Research Service, Southern Plains Agricultural Research Center, Food and Feed Safety Research Unit, College Station, TX 77845, USA.
Poole
Toni L
TL
Pultz
Nicole J
NJ
Hanrahan
Jennifer A
JA
Donskey
Curtis J
CJ
D000332:Aerobiosis; D000693:Anaerobiosis; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000898:Antibiosis; D001421:Bacteria, Anaerobic / Q000254:growth & development* / Q000378:metabolism*; D015169:Colony Count, Microbial; D016172:DNA Fingerprinting; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D016984:Enterococcus faecium / Q000235:genetics / Q000254:growth & development*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D014640:Vancomycin / Q000494:pharmacology; D020713:Vancomycin Resistance / Q000235:genetics*
D000900:Anti-Bacterial Agents; D014640:Vancomycin