title: Nickel sulphate and epoxy resin: differences in iron status and glutathione redox ration at the time of patch testing.
abstract: The reactive patch test reaction is a useful model to characterize oxidative stress in acute allergic contact dermatitis. This model was used to study oxidative stress in the skin of individuals allergic to nickel and epoxy resin. The study included six and five patients, respectively, whose skin was simultaneously biopsied from a positive patch test site and from an apparently healthy area. The biopsies were homogenized and used for determination of iron content, unbound iron binding capacity, diene conjugate levels, and glutathione redox ratio. A positive test reaction to 5% nickel sulphate was accompanied by 2.5-fold increase in iron level as compared to apparently healthy skin (P<0.1). The percentage saturation of iron-binding capacity and the glutathione redox ratio were significantly increased (P<0.01 and P<0.05, respectively). Reactive patch test responses to 1% epoxy resin were not accompanied by clear alterations in iron status or glutathione redox ratio. Our investigation showed that apart from oxidative burst caused by accumulation of inflammatory cells, hapten properties might also influence the oxidative stress status of the skin. The high incidence of nickel allergy may be attributed, at least in part, to the influence of nickel ions on the glutathione redox ratio and iron status of the skin.
authors.affiliation: Clinic of Dermatology, Tartu University, Estonia. Sirje.Kaur@kliinikum.ee
authors.forename: Sirje
authors.initials: S
authors.lastname: Kaur
authors.forename: Mihkel
authors.initials: M
authors.lastname: Zilmer
authors.forename: Maigi
authors.initials: M
authors.lastname: Eisen
authors.forename: Aune
authors.initials: A
authors.lastname: Rehema
authors.forename: Tiiu
authors.initials: T
authors.lastname: Kullisaar
authors.forename: Tiiu
authors.initials: T
authors.lastname: Vihalemm
authors.forename: Kersti
authors.initials: K
authors.lastname: Zilmer
mesh_terms: D000328:Adult; D017449:Dermatitis, Allergic Contact / Q000209:etiology*; D004853:Epoxy Resins / Q000009:adverse effects*; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D007501:Iron / Q000378:metabolism*; D015227:Lipid Peroxidation; D008875:Middle Aged; D009532:Nickel / Q000009:adverse effects*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D010328:Patch Tests
chemical_list: D004853:Epoxy Resins; C029938:nickel sulfate; D009532:Nickel; D007501:Iron; D005978:Glutathione