Aromatic amino acid aminotransferase activity and indole-3-acetic acid production by associative nitrogen-fixing bacteria.
In this work, we report the detection of aromatic amino acid aminotransferase (AAT) activity from cell-free crude extracts of nine strains of N(2)-fixing bacteria from three genera. Using tyrosine as substrate, AAT activity ranged in specific activity from 0.084 to 0.404 micromol min(-1)mg(-1). When analyzed under non-denaturating PAGE conditions; and using tryptophan, phenylalanine, tyrosine, and histidine as substrates Pseudomonas stutzeri A15 showed three isoforms with molecular mass of 46, 68 and 86 kDa, respectively; Azospirillum strains displayed two isoforms which molecular mass ranged from 44 to 66 kDa and Gluconacetobacter strains revealed one enzyme, which molecular mass was estimated to be much more higher than those of Azospirillum and P. stutzeri strains. After SDS-PAGE, some AAT activity was lost, indicating a differential stability of proteins. All the strains tested produced IAA, especially with tryptophan as precursor. Azospirillum strains produced the highest concentrations of IAA (16.5-38 microg IAA/mg protein), whereas Gluconacetobacter and P. stutzeri strains produced lower concentrations of IAA ranging from 1 to 2.9 microg/mg protein in culture medium supplemented with tryptophan. The IAA production may enable bacteria promote a growth-promoting effect in plants, in addition to their nitrogen fixing ability.
Pedraza
Raúl Osvaldo
RO
Centro de Investigaciones Microbiológicas, Instituto de Ciencias, Universidad Autónoma de Puebla, Apdo. Postal 1622. C.P. 72,000, Puebla Pue, Mexico. rpedraza@herrera.unt.edu.ar
Ramírez-Mata
Alberto
A
Xiqui
Ma Luisa
ML
Baca
Beatriz Eugenia
BE
D016949:Azospirillum / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D041821:Gluconacetobacter / Q000201:enzymology*; D006639:Histidine / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism* / Q000494:pharmacology; D008970:Molecular Weight; D009586:Nitrogen Fixation; D010649:Phenylalanine / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology; D044223:Pseudomonas stutzeri / Q000201:enzymology*; D013379:Substrate Specificity; D000637:Transaminases / Q000096:biosynthesis / Q000378:metabolism*; D014364:Tryptophan / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism
D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D014443:Tyrosine; D010649:Phenylalanine; D006639:Histidine; C030737:indoleacetic acid; D014364:Tryptophan; D000637:Transaminases; C019729:aromatic amino acid aminotransferase