title: p53-positive tumor-distant squamous epithelia of the head and neck reveal selective loss of chromosome 17.
abstract: OBJECTIVE
  The progression of clinically manifest premalignant lesions in the head and neck region to primary or second primary cancer is characterized by numerical and structural chromosomal aberrations. However, many tumors arise from histologically inconspicuous mucosal sites. The objective was to investigate whether chromosomal aberrations can be detected in tumor-distant mucosa and whether they can help predict the risk of second primary malignancy.
  
  
  METHODS
  A retrospective series of 72 clinically healthy, arbitrarily taken mucosal samples from 53 patients with squamous cell carcinoma of the head and neck was studied. A previous analysis of the p53 protein status had revealed both p53-positive and p53-negative samples.
  
  
  METHODS
  The samples were analyzed by fluorescence in situ hybridization (FISH) using centromeric probes specific for the chromosomes 1, 10, 17, and 18.
  
  
  RESULTS
  Tumor-distant mucosa generally showed increased numerical chromosomal aberrations, which consisted mainly of monosomies. In the group of patients with p53-positive epithelia, all aberrations including gains of chromosomes (trisomies, tetrasomies) were more frequent. Monosomy for chromosome 17 was most significantly and selectively enhanced in this group (Wilcoxon Scores [rank sum] test, P =.0002).
  
  
  CONCLUSIONS
  Detectable chromosomal aberrations occur in clinically healthy epithelia in patients at risk for secondary head and neck cancer. Specifically, unbalanced chromosome 17 monosomy in conjunction with p53 protein overexpression may constitute a valuable biomarker for progressive "field cancerization."
authors.affiliation: Molecular Biology Laboratory, Department of Otorhinolaryngology, University Hospital, School of Medicine, Heidelberg, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Wolf
authors.forename: Christa
authors.initials: C
authors.lastname: Flechtenmacher
authors.forename: Andreas
authors.initials: A
authors.lastname: Dietz
authors.forename: Hagen
authors.initials: H
authors.lastname: Weidauer
authors.forename: Ulrich
authors.initials: U
authors.lastname: Abel
authors.forename: Heinz
authors.initials: H
authors.lastname: Maier
authors.forename: Franz X
authors.initials: FX
authors.lastname: Bosch
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002872:Chromosome Deletion; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics; D004848:Epithelium; D005260:Female; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000473:pathology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D015342:DNA Probes; D016159:Tumor Suppressor Protein p53