Association of vitamin D receptor and estrogen receptor-alpha gene polymorphism with peak bone mass and bone size in Chinese women.
OBJECTIVE
To investigate if vitamin D receptor (VDR) gene Apa I polymorphism and estrogen receptor-alpha (ER-alpha) gene Pvu II, Xba I polymorphisms are related to bone mineral density (BMD), bone mineral content (BMC) and bone size in premenopausal Chinese women.


METHODS
The VDR Apa I genotype and ER-alpha Pvu II, Xba I genotype were determined by PCR-restriction fragment length polymorphism (RFLP) in 493 unrelated healthy women aged 20-40 years of Han nationality in Shanghai city. BMD (g/cm(2)), BMC (g), and bone areal size (BAS, cm(2) ) at lumbar spine 1-4 (L(1-4)) and proximal femur (femoral neck, trochanter and Ward's triangle) were measured by duel-energy X-ray absorptionmetry.


RESULTS
All allele frequencies did not deviate from Hardy-Weinberg equilibrium. After phenotypes were adjusted for age, height, and weight, a significant association was found between VDR Apa I genotype and BMC variation at L(1-4) and Ward's triangle (P<0.05), but not in BMD or BAS at lumbar spine and proximal femur. ER-a Pvu II, Xba I genotype was not related to BMC, BMD, and BAS at all sites.


CONCLUSIONS
The study suggested that Apa I polymorphism in VDR gene may influence on attainment and maintenance of peak bone mass in premenopausal Chinese women.
Qin
Yue-juan
YJ
Center for Preventing and Treating Osteoporosis, Osteoporosis Research Unit, Shanghai JiaoTong University Affiliated Sixth People s Hospital, Shanghai 200233, China. yuejuanQ@medmail.com.cn
Zhang
Zhen-lin
ZL
Huang
Qi-ren
QR
He
Jin-mei
JM
Hu
Yun-qiu
YQ
Zhao
Qi
Q
Lu
Jing-hui
JH
Li
Miao
M
Liu
Yu-juan
YJ
D000328:Adult; D044466:Asian People; D015519:Bone Density; D002681:China; D047628:Estrogen Receptor alpha; D005260:Female; D005269:Femur / Q000033:anatomy & histology; D005787:Gene Frequency; D006801:Humans; D008159:Lumbar Vertebrae / Q000033:anatomy & histology; D012150:Polymorphism, Restriction Fragment Length; D017697:Premenopause; D018167:Receptors, Calcitriol / Q000235:genetics*; D011960:Receptors, Estrogen / Q000235:genetics*
D047628:Estrogen Receptor alpha; D018167:Receptors, Calcitriol; D011960:Receptors, Estrogen