Liquid chromatographic assay for riluzole in mouse plasma and central nervous system tissues.
An isocratic, reversed-phase high-performance liquid chromatographic procedure (HPLC) was developed for determination of the neuroprotective agent riluzole in mice plasma, brain and spinal cord. The procedure is based on isolation of the compound and the internal standard from plasma and central nervous system tissues using a Bakerbond spe C8 cartridge, with satisfactory recovery and specificity. Separation was on a C18 column, coupled with an UV detector at 263 nm. The assay was linear over a wide range, with a lower limit of quantification of 100 ng ml(-1) or g(-1) using 0.1 ml of plasma and about 100mg of brain tissue. The precision and accuracy were within the acceptable limits for an HPLC assay. The method is currently used to support pharmacological studies of the activity of riluzole when given in combination with other potential neuroprotective agents in an animal model of familiar amyotrophic lateral sclerosis (SOD1-G93A transgenic mice).
Colovic
Milena
M
Istituto di Ricerche Farmacologiche Mario Negri, Via Eritrea 62, Milan 20157, Italy. colovic@marionegri.it
Zennaro
Eleonora
E
Caccia
Silvio
S
D000818:Animals; D002490:Central Nervous System / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D051379:Mice; D008822:Mice, Transgenic; D018696:Neuroprotective Agents / Q000097:blood / Q000493:pharmacokinetics*; D019782:Riluzole / Q000097:blood / Q000493:pharmacokinetics*; D013056:Spectrophotometry, Ultraviolet
D018696:Neuroprotective Agents; D019782:Riluzole