Plasmid condensation induced by cationic compounds: hydrophilic polylysine and amphiphilic cationic lipid.
The construction of an efficient carrier for genetic material is a major research objective that needs to be achieved before gene therapy can become a viable pharmacological approach. Artificial aggregates containing nucleic acids are one of the options for the systemic delivery of genetic information. The diversity of functions the aggregate is expected to fulfill necessitates its complex architecture. In order to obtain a complex supramolecular aggregate, formed from elements that are themselves complex molecules, appropriate procedures based on the detailed understanding of processes at the molecular level are required. In this study, we investigated how the various properties of cationic compounds affect nucleic acid condensation. The combination of two condensing agents, differing in their affinity towards water, when mixed with plasmids, resulted in aggregates which are resistant to enzymatic digestion and which form particles with well-defined size distributions. Such uniform and well-defined complexes may subsequently be further modified in order to obtain a fully functional genetic material carrier.
Chaszczewska-Markowska
Monika
M
Laboratory of Glycobiology, Department of Immunochemistry, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland.
Ugorski
Maciej
M
Langner
Marek
M
D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D008055:Lipids / Q000737:chemistry*; D010957:Plasmids / Q000737:chemistry* / Q000235:genetics; D011107:Polylysine / Q000737:chemistry*
D008055:Lipids; D011107:Polylysine