Effect of temperature, moisture, and carbon supplementation on lipase production by solid-state fermentation of soy cake by Penicillium simplicissimum.
The production of lipases by Penicillium simplicissimum using solid-state fermentation and soy cake as substrate was investigated. The effects of temperature, cake moisture, and carbon supplementation on lipase production were studied using a two-level experimental plan. Moisture, pH, and lipase activity were followed during fermentation. Statistical analysis of the results was performed to evaluate the effect of the studied variables on the maximum lipase activity. Incubation temperature was the variable that most affected enzyme activity, showing a negative effect. Moisture and carbon supplementation presented a positive effect on activity. It was possible to obtain lipase activity as high as 21 U/g of dry cake in the studied range of process variables.
Di Luccio
Marco
M
Department of Food Engineering, URI-Campus de Erechim, Av. Sete de Setembro 1621, Erechim, 99700-000, RS, Brazil. diluccio@uricer.edu.br
Capra
Fernando
F
Ribeiro
Najara P
NP
Vargas
Gean D L P
GD
Freire
Denise M G
DM
de Oliveira
Débora
D
D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000096:biosynthesis / Q000737:chemistry*; D000069463:Olive Oil; D010407:Penicillium / Q000378:metabolism*; D010938:Plant Oils; D013025:Glycine max / Q000382:microbiology*; D013696:Temperature; D013997:Time Factors
D003470:Culture Media; D000069463:Olive Oil; D010938:Plant Oils; D002244:Carbon; D008049:Lipase