[Effects of dry red wine on experimental atherosclerosis in rabbits].
OBJECTIVE
To study the effects of dry red wine in the different stages of experimental atherosclerosis (AS) at the cell, molecular and gene regulation levels in order to provide scientific basis for using dry red wine in the prevention of atherosclerosis.


METHODS
Blood vessel wall pathological changes, activity of NF-kB and the expressions of monocyte chemotactic protein-1 (MCP-1) and protein kinase C (PKC alpha) were observed in dietary induced atherosclerosis rabbit model by morphology study, electrophoretic mobility shift assay (EMSA), and in situ hybridyzation, and the effects of dry red wine intervention were examined.


RESULTS
Dry red wine significantly suppressed the proliferation of atherosclerosis intima and NF-kappaB activation (4w: 18.5 +/- 0.6 vs 13.7 +/- 0.3; 8w: 26 +/- 0.9 vs 17.8 +/- 0.5; 12w: 39.9 +/- 1.2 vs 27.8 +/- 0.8), and down-regulated the expressions of MCP-1 and PKC alpha.


CONCLUSIONS
The results confirmed that dry red wine could protect AS tissues and prolong its development by suppressing NF-kappaB activation, down-regulating the expressions of MCP-1 and PKC alpha, which may take part in pathogenesis of AS.
Cui
Hua
H
Department of Cardiology, Xingqiao Hospital, the Third Milltary Medical University, Chongqing 400037, China.
He
Zuo-yun
ZY
D000818:Animals; D001161:Arteriosclerosis / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D001808:Blood Vessels / Q000378:metabolism / Q000473:pathology; D018932:Chemokine CCL2 / Q000235:genetics; D004036:Diet, Atherogenic; D004195:Disease Models, Animal; D015870:Gene Expression; D017403:In Situ Hybridization; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D011493:Protein Kinase C / Q000235:genetics; D011817:Rabbits; D011897:Random Allocation; D014920:Wine
D018932:Chemokine CCL2; D016328:NF-kappa B; D011493:Protein Kinase C