title: The 26 Nudix hydrolases of Bacillus cereus, a close relative of Bacillus anthracis.
abstract: The genome of Bacillus cereus contains 26 Nudix hydrolase genes, second only to its closest relative, Bacillus anthracis which has 30. All 26 genes have been cloned, 25 have been expressed, and 21 produced soluble proteins suitable for analysis. Substrates for 16 of the enzymes were identified; these included ADP-ribose, diadenosine polyphosphates, sugar nucleotides, and deoxynucleoside triphosphates. One of the enzymes was a CDP-choline pyrophosphatase, the first Nudix hydrolase active on this substrate. Furthermore, as a result of this and previous work we have identified a new sub-family of the Nudix hydrolase superfamily recognizable by a specific amino acid motif outside of the Nudix box.
authors.affiliation: Department of Biology and The McCollum-Pratt Institute, The Johns Hopkins University, Baltimore, Maryland 21218, USA.
authors.forename: WenLian
authors.initials: W
authors.lastname: Xu
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Dunn
authors.forename: Candice R
authors.initials: CR
authors.lastname: Jones
authors.forename: Gehaan
authors.initials: G
authors.lastname: D'Souza
authors.forename: Maurice J
authors.initials: MJ
authors.lastname: Bessman
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001408:Bacillus anthracis / Q000201:enzymology*; D001409:Bacillus cereus / Q000201:enzymology*; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D007700:Kinetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D011755:Pyrophosphatases / Q000737:chemistry*; D013045:Species Specificity; D014530:Uridine Diphosphate / Q000737:chemistry
chemical_list: D014530:Uridine Diphosphate; D011755:Pyrophosphatases