Prostaglandins are powerful inducers of NGF and BDNF production in mouse astrocyte cultures.
We found that prostaglandin (PG) D(2) and PGE(2), which are major PGs in the brain of mammals, powerfully induced the secretion of nerve growth factor (NGF) from cultured mouse astrocytes; PGE(2) or PGD(2) induced an approximately 12- or 19-fold increase in NGF secretion after a 24-h incubation, respectively. Moreover, it was found that the sequential metabolites of PGD(2), PGJ(2), Delta(12)-PGJ(2), and 15-deoxy-Delta(12,14)-PGJ(2), induced the NGF secretion to the culture medium strikingly (60-98-fold of the control after a 24-h incubation). NGF secretion induced by the J(2) series of PGs was accompanied by the increased expression of NGF mRNA. These PGs also stimulated the secretion/synthesis of brain-derived neurotrophic factor (BDNF). Our findings suggest that PGs play a neuroprotective role by inducing NGF and BDNF production in the central nervous system.
Toyomoto
Misao
M
Department of Biochemistry, Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka 569-1094, Japan.
Ohta
Mitsuhiro
M
Okumura
Kohji
K
Yano
Hiroe
H
Matsumoto
Kaori
K
Inoue
Seiji
S
Hayashi
Kyozo
K
Ikeda
Kiyoshi
K
D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism*; D051379:Mice; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism*; D015230:Prostaglandin D2 / Q000031:analogs & derivatives* / Q000235:genetics / Q000378:metabolism*
D019208:Brain-Derived Neurotrophic Factor; C037112:9-deoxy-delta-9-prostaglandin D2; D020932:Nerve Growth Factor; D015232:Dinoprostone; D015230:Prostaglandin D2