Comparisons of protocols for decontamination of environmental ice samples for biological and molecular examinations.
Drilling and laboratory manipulations of glacial ice cores introduce contemporary microbes and biomolecules onto the cores. We report herein a systematic comparative study of several decontamination protocols. Only treatment with 5% sodium hypochlorite eliminated all external contaminating microbes and nucleic acids while maintaining the integrity of those within the cores.
Rogers
S O
SO
Department of Biological Sciences, Bowling Green State University, Bowling Green, Ohio 43403, USA. srogers@bgnet.bgsu.edu
Theraisnathan
V
V
Ma
L J
LJ
Zhao
Y
Y
Zhang
G
G
Shin
S-G
SG
Castello
J D
JD
Starmer
W T
WT
D015169:Colony Count, Microbial; D003666:Decontamination / Q000379:methods*; D007053:Ice; D008828:Microbiological Techniques; D012973:Sodium Hypochlorite; D014871:Water Microbiology
D007053:Ice; D012973:Sodium Hypochlorite