title: In vivo non-specific binding of lambda CI and Cro repressors is significant.
abstract: We propose a thermodynamic model that includes the non-specific binding of the lambda phage regulatory proteins CI and Cro. By fitting the model to experimental in vivo data on activities of the two promoters P(RM) and P(R) versus concentration, we estimate the free energy upon non-specific binding to be -4.1+/-0.9 kcal/mol for CI and -4.2+/-0.8 kcal/mol for Cro. For concentrations >100 nM of CI or Cro, we find that >50% of these proteins are non-specifically bound. In particular, in a lysogen (approximately 250 CI monomeric equivalents per cell) nearly 90% of CI is non-specifically bound.
authors.affiliation: NORDITA, Blegdamsvej 17, DK-2100 Copenhagen, Denmark. audunba@nordita.dk
authors.forename: Audun
authors.initials: A
authors.lastname: Bakk
authors.forename: Ralf
authors.initials: R
authors.lastname: Metzler
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral; D014644:Genetic Variation; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013816:Thermodynamics; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D012097:Repressor Proteins; D014764:Viral Proteins