title: Molecular interconversion behaviour in comprehensive two-dimensional gas chromatography.
abstract: Comprehensive two-dimensional gas chromatography (GC x GC) is shown to provide information on dynamic molecular behaviour (interconversion), with the interconversion process occurring on both columns in the coupled-column experiment. The experiment requires suitable adjustment of both experimental conditions and relative dimensions of each of the columns. In this case, a longer column than normally employed in GC x GC allows sufficient retention duration on the second column, which permits the typical plateau-shape recognised for the interconversion process to be observed. The extent of interconversion depends on prevailing temperature, retention time, and the phase type. Polyethylene glycol-based phases were found to result in high interconversion kinetics, although terephthalic acid-terminated polyethylene glycol had a lesser extent of interconversion. Much less interconversion was seen for phenyl-methylpolysiloxane and cyclodextrin phases. This suggests that for the oximes, interconversion largely occurs in the stationary phase. Examples of different extents of interconversion in both dimensions are shown, including peak coalescence on the first column with little interconversion on the second column.
authors.affiliation: Department of Applied Chemistry, Australian Centre for Research on Separation Science, RMIT University, City Campus, GPO Box 2476V, Melbourne, Vic. 3001, Australia. philip.marriott@rmit.edu.au
authors.forename: Philip
authors.initials: P
authors.lastname: Marriott
authors.forename: Kornkanok
authors.initials: K
authors.lastname: Aryusuk
authors.forename: Robert
authors.initials: R
authors.lastname: Shellie
authors.forename: Danielle
authors.initials: D
authors.lastname: Ryan
authors.forename: Kanit
authors.initials: K
authors.lastname: Krisnangkura
authors.forename: Volker
authors.initials: V
authors.lastname: Schurig
authors.forename: Oliver
authors.initials: O
authors.lastname: Trapp
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D007700:Kinetics