title: Knock-down of the 37-kDa/67-kDa laminin receptor in mouse brain by transgenic expression of specific antisense LRP RNA.
abstract: The 37-kDa/67-kDa laminin receptor (LRP/LR) plays a major role in the propagation of PrPSc, the abnormal form of the prion protein. In order to ablate the expression of LRP/LR in mouse brain we generated transgenic mice ectopically expressing antisense LRP RNA in the brain under control of the neuron-specific enolase (NSE) promoter. Hemizygous transgenic mice TgN(NSEasLRP)2 showed a significant reduction of LRP/LR protein levels in hippocampal and cerebellar brain regions. These mice might act as powerful tools to investigate the role of the laminin receptor in scrapie pathogenesis.
authors.affiliation: Laboratorium für Molekulare Biologie Genzentrum Institut für Biochemie der LMU München, Feodor-Lynen-Strasse 25, D-81377 Munich, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Leucht
authors.forename: Karen
authors.initials: K
authors.lastname: Vana
authors.forename: Ingrid
authors.initials: I
authors.lastname: Renner-Müller
authors.forename: Dominique
authors.initials: D
authors.lastname: Dormont
authors.forename: Corinne Ida
authors.initials: CI
authors.lastname: Lasmézas
authors.forename: Eckhard
authors.initials: E
authors.lastname: Wolf
authors.forename: Stefan
authors.initials: S
authors.lastname: Weiss
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002460:Cell Line; D015870:Gene Expression; D051379:Mice / Q000235:genetics*; D008822:Mice, Transgenic; D008970:Molecular Weight; D010751:Phosphopyruvate Hydratase / Q000235:genetics; D011401:Promoter Regions, Genetic; D016372:RNA, Antisense / Q000378:metabolism*; D051381:Rats; D017474:Receptors, Laminin / Q000235:genetics / Q000378:metabolism*
chemical_list: D016372:RNA, Antisense; D017474:Receptors, Laminin; D010751:Phosphopyruvate Hydratase