Dissemination of CTX-M-type beta-lactamases among clinical isolates of Enterobacteriaceae in Paris, France.
We analyzed 19 clinical isolates of the family Enterobacteriaceae (16 Escherichia coli isolates and 3 Klebsiella pneumoniae isolates) collected from four different hospitals in Paris, France, from 2000 to 2002. These strains had a particular extended-spectrum cephalosporin resistance profile characterized by a higher level of resistance to cefotaxime and aztreonam than to ceftazidime. The bla(CTX-M) genes encoding these beta-lactamases were involved in this resistance, with a predominance of bla(CTX-M-15). Ten of the 19 isolates produced both TEM-1- and CTX-M-type enzymes. One strain (E. coli TN13) expressed CMY-2, TEM-1, and CTX-M-14. bla(CTX-M) genes were found on large plasmids. In 15 cases the same insertion sequence, ISEcp1, was located upstream of the 5' end of the bla(CTX-M) gene. In one case we identified an insertion sequence designated IS26. Examination of the other three bla(CTX-M) genes by cloning, sequencing, and PCR analysis revealed the presence of a complex sul1-type integron that includes open reading frame ORF513, which carries the bla gene and the surrounding DNA. Five isolates had the same plasmid DNA fingerprint, suggesting clonal dissemination of CTX-M-15-producing strains in the Paris area.
Eckert
C
C
Laboratoire de Bactériologie, UPRES EA 2392, UFR Saint-Antoine, Université Paris VI, France.
Gautier
V
V
Saladin-Allard
M
M
Hidri
N
N
Verdet
C
C
Ould-Hocine
Z
Z
Barnaud
G
G
Delisle
F
F
Rossier
A
A
Lambert
T
T
Philippon
A
A
Arlet
G
G
D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000235:genetics*; D004756:Enterobacteriaceae Infections / Q000453:epidemiology / Q000382:microbiology*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005602:France / Q000453:epidemiology; D006801:Humans; D007525:Isoelectric Focusing; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics; D008826:Microbial Sensitivity Tests; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001618:beta-Lactamases / Q000235:genetics*
D004269:DNA, Bacterial; D001618:beta-Lactamases