Development of an experimental system for evaluation of stress effect on ethyl alcohol metabolism using radiorespirometric analysis in rat.
We attempted to develop an experimental system for evaluation of stress effect on ethyl alcohol metabolism by the radiorespirometric analysis using [(14)C]ethyl alcohol. Each rat was immobilized in an adjustable restraint device for 3 h. The excretion of (14)CO(2) in the breath was significantly decreased by restraint stress. The liver alcohol dehydrogenase activity was also decreased by restraint stress. We think that radiorespirometric analysis may be useful for the evaluation of stress effect on real metabolism of ethyl alcohol in the liver.
Maruko
Akiko
A
Department of Radiopharmacy, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan.
Ohtake
Yosuke
Y
Suzuki
Atsuko
A
Sato
Naoko
N
Ohkubo
Yasuhito
Y
D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000818:Animals; D001944:Breath Tests / Q000379:methods*; D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes; D000431:Ethanol / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical; D013315:Stress, Psychological / Q000378:metabolism*
D002250:Carbon Radioisotopes; D002245:Carbon Dioxide; D000431:Ethanol; D000426:Alcohol Dehydrogenase