title: Cell immersion and cell dipping in microfluidic devices.
abstract: Combining deflective dielectrophoretic barriers with controlled pressure driven liquid flows in microfluidic devices allows accurate handling of particles such as biological cells in suspensions. Working towards cell-based lab-on-a-chip applications, a platform permitting rapid testing of devices having different dielectrophoretic and fluidic subunits was developed. The performance of such a system is shown in the cases of (A) flooding a small number of immobilised cells with a dye and (B) transient buffer swapping of a large number of cells in flow. The transition times for moving cells from one reagent to the other are below 0.5 s in the case of flow-through cell dipping.
authors.affiliation: Swiss Federal Institute of Technology, EPFL, STI-LMIS, CH-1015 Lausanne, Switzerland. urban.seger@epfl.ch
authors.forename: Urban
authors.initials: U
authors.lastname: Seger
authors.forename: Shady
authors.initials: S
authors.lastname: Gawad
authors.forename: Robert
authors.initials: R
authors.lastname: Johann
authors.forename: Arnaud
authors.initials: A
authors.lastname: Bertsch
authors.forename: Philippe
authors.initials: P
authors.lastname: Renaud
mesh_terms: D001709:Biotechnology / Q000295:instrumentation / Q000379:methods; D002021:Buffers; D018914:Cells, Immobilized; D004396:Coloring Agents; D003584:Cytological Techniques / Q000295:instrumentation / Q000379:methods*; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D004912:Erythrocytes; D006801:Humans; D007700:Kinetics; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods*
chemical_list: D002021:Buffers; D004396:Coloring Agents