title: Stable transduction of large DNA by high-capacity adeno-associated virus/adenovirus hybrid vectors.
abstract: Viral vectors with high cloning capacity and host chromosomal integration ability are in demand for the efficient and permanent genetic modification of target cells with large DNA molecules. We have generated a hybrid gene transfer vehicle consisting of recombinant adeno-associated virus (AAV) replicative intermediates packaged in adenovirus (Ad) capsids. This arrangement allows cell cycle-independent nuclear delivery of recombinant AAV genomes with lengths considerably above the maximum size (i.e., 4.7 kb) that can be accommodated within AAV capsids. Here we show that high-capacity AAV/Ad hybrid vector gene transfer mediates cellular genomic integration of large fragments of foreign DNA and accomplishes stable long-term transgene expression in rapidly proliferating cells. Southern blot and polymerase chain reaction analyses of chromosomal DNA extracted from clones of stably transduced cells revealed that most of them contained a single copy of the full-length hybrid vector genome with AAV inverted terminal repeat (ITR) sequences at both ends. The high-capacity AAV/Ad hybrid vector system can thus be used for the transfer and expression of transgenes that cannot be delivered by conventional integrating viral vectors.
authors.affiliation: Gene Therapy Section, Department of Molecular Cell Biology, Leiden University Medical Center, 2333 AL Leiden, The Netherlands. m.goncalves@lumc.nl
authors.forename: Manuel A F V
authors.initials: MA
authors.lastname: Gonçalves
authors.forename: Ietje
authors.initials: I
authors.lastname: van der Velde
authors.forename: Shoshan
authors.initials: S
authors.lastname: Knaän-Shanzer
authors.forename: Dinko
authors.initials: D
authors.lastname: Valerio
authors.forename: Antoine A F
authors.initials: AA
authors.lastname: de Vries
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D002213:Capsid; D004279:DNA, Viral; D000229:Dependovirus / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D011995:Recombination, Genetic; D020079:Terminal Repeat Sequences; D014161:Transduction, Genetic / Q000379:methods; D016662:Virus Integration
chemical_list: D004279:DNA, Viral