title: The origin of the sodium-dependent NADH oxidation by the respiratory chain of Klebsiella pneumoniae.
abstract: Properties of Klebsiella pneumoniae respiratory chain enzymes catalyzing NADH oxidation have been studied. Using constructed K. pneumoniae mutant strains, it was shown that three enzymes belonging to different families of NADH:quinone oxidoreductases operate in this bacterium. The NDH-2-type enzyme is not coupled with energy conservation, the NDH-1-type enzyme is a primary proton pump, and the NQR-type enzyme is homologous to the sodium-motive NADH dehydrogenase of Vibrio and is shown to be a primary Na(+) pump. It is concluded that the NQR-type enzyme, not the NDH-1-type enzyme, catalyzes sodium-dependent NADH oxidation in K. pneumoniae.
authors.affiliation: Department of Molecular Energetics of Microorganisms, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russia.
authors.forename: Yulia V
authors.initials: YV
authors.lastname: Bertsova
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Bogachev
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D004579:Electron Transport; D042967:Electron Transport Complex I; D007700:Kinetics; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D009154:Mutation; D009245:NADH Dehydrogenase / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D017494:Proton Pumps; D011522:Protons; D011808:Quinone Reductases / Q000378:metabolism; D012964:Sodium / Q000378:metabolism*; D014733:Vibrio / Q000201:enzymology
chemical_list: D001426:Bacterial Proteins; D017494:Proton Pumps; D011522:Protons; D012964:Sodium; D010088:Oxidoreductases; D009247:NADH, NADPH Oxidoreductases; D011808:Quinone Reductases; D009245:NADH Dehydrogenase; D042967:Electron Transport Complex I