Microdissection: a method developed to investigate mechanisms involved in transmissible spongiform encephalopathy pathogenesis.
BACKGROUND
The transmissible spongiform encephalopathies (TSEs) are a group of neurodegenerative diseases affecting both human and animals. The neuroanatomical changes which occur in the central nervous system (CNS) of TSE infected animals include vacuolation, gliosis, neuronal loss and the deposition of a disease specific protein, PrPSc. Experimental murine models of scrapie, a TSE of sheep, have revealed that pathology may be confined to specific brain areas with targeting of particular neuronal subsets depending on route of injection and scrapie isolate. To assess the biochemical changes which are taking place in these targeted areas it was necessary to develop a reliable sampling procedure (microdissection) which could be used for a variety of tests such as western blotting and magnetic resonance spectroscopy.


METHODS
The method described is for the microdissection of murine brains. To assess the usefulness of this dissection technique for producing similar sample types for analysis by various down-stream biochemical techniques, the areas dissected were analysed for PrPSc by western blotting and compared to immunocytochemical (ICC) techniques.


RESULTS
Results show that the method generates samples yielding a consistent protein content which can be analysed for PrPSc. The areas in which PrPSc is found by western blotting compares well with localisation visualised by immunocytochemistry.


CONCLUSIONS
The microdisssection method described can be used to generate samples suitable for a range of biochemical techniques. Using these samples a range of assays can be carried out which will help to elucidate the molecular and cellular mechanisms underlying TSE pathogenesis. The method would also be useful for any study requiring the investigation of discrete areas within the murine brain.
Barr
Janice B
JB
BBSRC & MRC Neuropathogenesis Unit, Institute for Animal Health, Edinburgh, UK. janice.barr@bbsrc.ac.uk
Somerville
Robert A
RA
Chung
Yuen-Li
YL
Fraser
Janet R
JR
D000818:Animals; D015153:Blotting, Western / Q000379:methods*; D002417:Cattle; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D018620:PrPSc Proteins / Q000032:analysis*; D017096:Prion Diseases / Q000378:metabolism*
D018620:PrPSc Proteins