title: Molecular diagnosis of human adenoviruses d and e by a phylogeny-based classification method using a partial hexon sequence.
abstract: Human adenoviruses (HAdVs) are the major causes of a variety of acute illnesses. Virus isolation and neutralization tests are usually done to identify the causative virus, but these tests are labor-intensive and time-consuming, and standardized antisera are in limited supply. This study investigated a rapid and reliable method of virus identification based on PCR and phylogenetic analysis. The phylogenetic tree constructed by neighbor joining on the basis of the newly determined partial hexon sequences from 33 prototypes of HAdV-D and -E, along with 11 available prototypes of HAdV-A to -C and -F from GenBank, allowed HAdVs to be grouped into six distinct clusters. These clusters correspond closely to the six newly designated species, HAdV-A to -F. The partial hexon sequences of 57 isolates from patients with acute conjunctivitis obtained over 20 years plus those of 44 prototype strains were analyzed. Each isolate formed a monophyletic cluster along with its respective prototype strain, allowing serotype identification. Partial-hexon-based classification appears to be an effective tool for studying the molecular epidemiology of HAdVs.
authors.affiliation: Research and Development Department, Mitsubishi Kagaku Bio-Clinical Laboratories, Inc., Shimura 3-30-1, Itabashi-ku, Tokyo 174-8555, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Shimada
authors.forename: Toshihide
authors.initials: T
authors.lastname: Ariga
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Tagawa
authors.forename: Koki
authors.initials: K
authors.lastname: Aoki
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Ohno
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ishiko
mesh_terms: D000258:Adenovirus Infections, Human / Q000175:diagnosis* / Q000821:virology; D000260:Adenoviruses, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D036022:Capsid Proteins / Q000737:chemistry* / Q000235:genetics; D003236:Conjunctivitis, Viral / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012703:Serotyping
chemical_list: D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus