title: Fluorometric assay protocol for protease-catalyzed transesterification reactions in organic solvents.
abstract: A flourometric assay protocol for a subtilisin-catalyzed transesterification reaction in n-hexane has been developed. The method makes use of a Michael acceptor that forms a fluorescent adduct with thiophenol, one of the products generated in the transesterification reaction. The method may be employed for screening a biocatalyst useful for transesterification reactions in organic solvents and for optimizing the transesterification reaction conditions.
authors.affiliation: Center for Integrated Molecular Systems, and National Research Laboratory of Chirotechnology, Department of Chemistry, Pohang University of Science and Technology, San 31 Hyojadong, Pohang 790-784, Korea.
authors.forename: Min Su
authors.initials: MS
authors.lastname: Han
authors.forename: Sang Oh
authors.initials: SO
authors.lastname: Jung
authors.forename: Mahn-Joo
authors.initials: MJ
authors.lastname: Kim
authors.forename: Dong H
authors.initials: DH
authors.lastname: Kim