Headspace solid-phase micro-extraction and gas chromatography with micro-electron capture detection for the measurement of p,p'-DDE in rat whole blood and hair.
A sensitive, specific, and reproducible capillary gas chromatography (GC) with micro-electron capture detection (micro-ECD) method using headspace solid-phase micro-extraction (HS-SPME) for the quantitative analysis of p,p'-DDE in rat whole blood and hair was developed. A 100 microm polydimethylsiloxane (PDMS) phase was used for the extraction. The obtained detection limits were 0.003 ng/mg and 0.004 ng/mg in whole blood and hair, respectively, at a signal-to-noise ratio of 3 (S/N = 3). Linearity was obtained in the ranges 0.003-2.0 ng/mg and 0.004-2.0 ng/mg in whole blood and hair, respectively, and the correlation coefficients of whole blood and hair were greater than 0.998. This method was successfully applied to the analysis of p,p'-DDE in rat whole blood and rat hair after an oral dose of p,p'-DDE.
Kusakabe
Takahiko
T
Department of Forensic Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
Saito
Takeshi
T
Takeichi
Sanae
S
D000818:Animals; D001769:Blood; D002849:Chromatography, Gas / Q000379:methods*; D003633:Dichlorodiphenyl Dichloroethylene / Q000032:analysis*; D006197:Hair / Q000737:chemistry*; D007306:Insecticides / Q000032:analysis*; D015394:Molecular Structure; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature
D007306:Insecticides; D003633:Dichlorodiphenyl Dichloroethylene