title: Apoptosis of crypt cells and lymphocytes in gut-associated lymphoid tissues during small intestinal graft rejection in rats.
abstract: BACKGROUND
  We investigated the extent of apoptosis in crypt cells and Peyer's patches (PPs) during small bowel allograft rejection in rats to examine whether the Fas/FasL pathway participates in apoptosis within grafts during rejection.
  
  
  METHODS
  Orthotopic small bowel transplantation with portocaval drainage was performed from Brown Norway to Lewis (LEW) rats. Isografted (LEW --> LEW) and nontransplanted animals served as the controls. Animals were sacrificed on days 3, 5, on 7 after SBT (each n = 5). An in situ end-labeling (ISEL) technique was used to detect apoptotic cells. Indirect immunoperoxidase staining was also performed using monoclonal antibodies against rat Fas or Fas-L.
  
  
  RESULTS
  The number of ISEL-positive enterocytes in the allografts increased significantly on days 3, 5, and 7. Similarly, in the PPs of the allografts, the number of ISEL-positive mononuclear cells increased significantly on days 3, 5, and 7. On day 7 the number of Fas- and FasL-positive enterocytes were increased significantly in the allografts compared with the nontransplanted controls. Similarly, in the PPs, Fas- and FasL-positive mononuclear cells also increased significantly on day 7 in the allograft.
  
  
  CONCLUSIONS
  Although an increase, number of apoptotic enterocytes and lymphocytes were observed in the early phase, activation of Fas/FasL system occurred during the late phase of small bowel graft rejection. These findings suggest that both rejection-associated and sepsis-induced forms of apoptosis may be associated with small bowel graft rejection.
authors.affiliation: First Department of Surgery, Nihon University School of Medicine, Tokyo, Japan. Park8@med.nihon-u.ac.be
authors.forename: Y J
authors.initials: YJ
authors.lastname: Park
authors.forename: S
authors.initials: S
authors.lastname: Fujisaki
authors.forename: K
authors.initials: K
authors.lastname: Kimizuka
authors.forename: K
authors.initials: K
authors.lastname: Shugito
authors.forename: R
authors.initials: R
authors.lastname: Tomita
authors.forename: M
authors.initials: M
authors.lastname: Fukuzawa
authors.forename: K
authors.initials: K
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053222:Fas Ligand Protein; D006084:Graft Rejection / Q000473:pathology*; D007413:Intestinal Mucosa / Q000473:pathology / Q000601:surgery*; D007421:Intestine, Small / Q000473:pathology / Q000601:surgery*; D008214:Lymphocytes / Q000473:pathology* / Q000502:physiology; D008562:Membrane Glycoproteins / Q000032:analysis; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D014184:Transplantation, Homologous / Q000473:pathology*; D014185:Transplantation, Isogeneic / Q000473:pathology*; D019014:fas Receptor / Q000032:analysis
chemical_list: D053222:Fas Ligand Protein; C505662:Faslg protein, rat; D008562:Membrane Glycoproteins; D019014:fas Receptor