Analysis of an engineered Salmonella flagellar fusion protein, FliR-FlhB.
Salmonella FliR and FlhB are membrane proteins necessary for flagellar export. In Clostridium a fliR-flhB fusion gene exists. We constructed a similar Salmonella fusion gene which is able to complement fliR, flhB, and fliR flhB null strains. Western blotting revealed that the FliR-FlhB fusion protein retains the FlhB protein's cleavage properties. We conclude that the FliR and FlhB proteins are physically associated in the wild-type Salmonella basal body, probably in a 1:1 ratio.
Van Arnam
John S
JS
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA.
McMurry
Jonathan L
JL
Kihara
May
M
Macnab
Robert M
RM
D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D015153:Blotting, Western; D005407:Flagella / Q000378:metabolism*; D005816:Genetic Complementation Test; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics; D012475:Salmonella / Q000378:metabolism*
D001426:Bacterial Proteins; C085668:FlhB protein, Bacteria; C085248:FliR protein, Bacteria; D008565:Membrane Proteins