title: PCR hot-start using duplex primers.
abstract: A new technique of PCR hot-start using duplex primers has been developed which can decrease the undesirable products arising throughout PCR amplification thereby giving better results than a manual hot-start method.
authors.affiliation: State Key Laboratory, of Functional Polymer Materials for Adsorption and Separation, Chemical School, Nankai University, Tianjin 300071, P.R. China. kongdem@eyou.com
authors.forename: Deming
authors.initials: D
authors.lastname: Kong
authors.forename: Hanxi
authors.initials: H
authors.lastname: Shen
authors.forename: Yanping
authors.initials: Y
authors.lastname: Huang
authors.forename: Huaifeng
authors.initials: H
authors.lastname: Mi
mesh_terms: D001709:Biotechnology / Q000379:methods*; D004247:DNA / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D006515:Hepatitis B virus / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods*
chemical_list: D017931:DNA Primers; D004247:DNA