title: Facile synthesis of metal-chelating peptides on chip for protein array.
abstract: A unique peptide sequence of HGGHHG screening from a combinatorial synthetic peptide library showed a good chelating ability to bind a transition metal on a chip better than hexa-His peptide. It was directly conjugated with a His-Tagged proteins onto a chip in a mild aqueous solution and can be used this chip as a high throughput technique for protein array in order to detect and purify the His-Tagged proteins.
authors.affiliation: ALPS Biotech Co. Ltd, Taipei, Taiwan.
authors.forename: Ching-Wen
authors.initials: CW
authors.lastname: Cheng
authors.forename: Kuo-Chin
authors.initials: KC
authors.lastname: Lin
authors.forename: Fu-Ming
authors.initials: FM
authors.lastname: Pan
authors.forename: Supachok
authors.initials: S
authors.lastname: Sinchaikul
authors.forename: Chi-Huey
authors.initials: CH
authors.lastname: Wong
authors.forename: Wei-Chih
authors.initials: WC
authors.lastname: Su
authors.forename: Ching-Hsiang
authors.initials: CH
authors.lastname: Hsu
authors.forename: Shui-Tein
authors.initials: ST
authors.lastname: Chen
mesh_terms: D002614:Chelating Agents / Q000138:chemical synthesis* / Q000378:metabolism; D002846:Chromatography, Affinity / Q000379:methods; D003300:Copper / Q000737:chemistry; D006639:Histidine / Q000737:chemistry; D008670:Metals / Q000737:chemistry*; D019151:Peptide Library; D010455:Peptides / Q000138:chemical synthesis* / Q000378:metabolism; D040081:Protein Array Analysis / Q000379:methods*; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D015032:Zinc / Q000737:chemistry
chemical_list: D002614:Chelating Agents; D008670:Metals; D019151:Peptide Library; D010455:Peptides; D011994:Recombinant Proteins; D006639:Histidine; D003300:Copper; D015032:Zinc