Isolation and characterization of site-specific DNA-methyltransferases from Bacillus coagulans K.
Two site-specific DNA methyltransferases, M.BcoKIA and M.BcoKIB, were isolated from the thermophilic strain Bacillus coagulans K. Each of the methylases protects the recognition site 5'-CTCTTC-3'/5'-GAAGAG-3' from cleavage with the cognate restriction endonuclease BcoKI. It is shown that M.BcoKIB is an N6-adenine specific methylase and M.BcoKIA is an N4-cytosine specific methylase. According to bisulfite mapping, M.BcoKIA methylates the first cytosine in the sequence 5'-CTCTTC-3'.
Svadbina
I V
IV
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russia.
Zelinskaya
N V
NV
Kovalevskaya
N P
NP
Zheleznaya
L A
LA
Matvienko
N I
NI
D000225:Adenine / Q000737:chemistry*; D001407:Bacillus / Q000201:enzymology*; D002853:Chromatography, Liquid; D003596:Cytosine / Q000737:chemistry*; D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000737:chemistry* / Q000302:isolation & purification*; D015257:DNA-Cytosine Methylases / Q000737:chemistry* / Q000302:isolation & purification*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry*; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000737:chemistry* / Q000302:isolation & purification*; D013379:Substrate Specificity
D003596:Cytosine; D015254:DNA Modification Methylases; D015257:DNA-Cytosine Methylases; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); C057073:endodeoxyribonuclease Ksp632I; D015252:Deoxyribonucleases, Type II Site-Specific; D000225:Adenine