title: Altered gene expression in the adult brain of fyn-deficient mice.
abstract: 1. Fyn, a member of Src-family tyrosine kinases, is implicated in both brain development and adult brain function. Recent studies have identified some Fyn substrates, however, little is known about the transcriptional targets for Fyn mediated signaling pathways. In the present study, we sought to identify targets downstream of Fyn in vivo. 2. We compared genes expressed in adult hippocampi of wild-type and fyn-deficient mice using gene chips containing more than 12,000 genes. 3. The results showed that 559 transcripts were expressed differentially between these mice. Expression of 20 genes including a substantial number of myelin-associated genes was strongly repressed in fyn-deficient mice. 4. Reduced expression of these myelin-associated genes, such as MBP and MOG, in fyn-deficient mice was also confirmed by real-time PCR and northern blotting, arguing that Fyn is important for function and development of oligodendrocytes. 5. Further analysis of the genes that are differently expressed in fyn-deficient mice may shed light on the molecular mechanism by which Fyn regulates adult neural function.
authors.affiliation: Division of Oncology, Department of Cancer Biology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors.forename: June
authors.initials: J
authors.lastname: Goto
authors.forename: Tohru
authors.initials: T
authors.lastname: Tezuka
authors.forename: Takanobu
authors.initials: T
authors.lastname: Nakazawa
authors.forename: Nobuo
authors.initials: N
authors.lastname: Tsukamoto
authors.forename: Takahisa
authors.initials: T
authors.lastname: Nakamura
authors.forename: Rieko
authors.initials: R
authors.lastname: Ajima
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Yokoyama
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Ohta
authors.forename: Misao
authors.initials: M
authors.lastname: Ohki
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D001921:Brain / Q000254:growth & development* / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000235:genetics*; D006624:Hippocampus / Q000254:growth & development / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D004676:Myelin Basic Protein / Q000235:genetics / Q000378:metabolism; D009185:Myelin Proteins; D009186:Myelin Sheath / Q000235:genetics / Q000378:metabolism*; D018992:Myelin-Associated Glycoprotein / Q000235:genetics / Q000378:metabolism; D063308:Myelin-Oligodendrocyte Glycoprotein; D020411:Oligonucleotide Array Sequence Analysis; D011518:Proto-Oncogene Proteins / Q000172:deficiency* / Q000235:genetics; D051076:Proto-Oncogene Proteins c-fyn
chemical_list: C571327:Mog protein, mouse; D004676:Myelin Basic Protein; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D011518:Proto-Oncogene Proteins; C494960:Fyn protein, mouse; D051076:Proto-Oncogene Proteins c-fyn