title: Continuous intra-amygdalar infusion of GABA in the amygdala kindling model of epilepsy in rat.
abstract: OBJECTIVE
  To explore the effect of continuous intra-amygdalar infusion of GABA in the amygdala kindling model of epilepsy in rat.
  
  
  METHODS
  An electrode and cannula complex was implanted in adult male Wistar rats, the electrode being targeted to the left basolateral amygdala. The animals were subjected to a standard kindling procedure. Osmotic minipumps filled with either GABA or mannitol were connected to cannulas and allowed to infuse during 7 days. Kindling experiments measuring after-discharge and seizure thresholds, seizure severity and duration, and behavioral toxicity were performed before, during and after the drug infusion period.
  
  
  RESULTS
  Both after-discharge and seizure thresholds were significantly increased both during and after GABA infusion with respect to pre-infusion controls, while the group receiving mannitol showed no significant differences. Seizure duration was not affected by GABA infusion. Seizures were either completely blocked or fully developed.
  
  
  CONCLUSIONS
  Direct continuous GABA infusion within the epileptogenic focus raises the seizure thresholds without affecting the generalized components in the amygdala kindling model of epilepsy.
authors.affiliation: Servicio de Neurología, Consorcio Hospital General Universitario de Valencia, Valencia 46014, Spain. Juan.A.Barcia@uv.es
authors.forename: Juan A
authors.initials: JA
authors.lastname: Barcia
authors.forename: Ascensión
authors.initials: A
authors.lastname: Castillo
authors.forename: Rafael
authors.initials: R
authors.lastname: Gutiérrez
authors.forename: José M
authors.initials: JM
authors.lastname: Gallego
authors.forename: Laura
authors.initials: L
authors.lastname: Ortiz
authors.forename: M Eugenia
authors.initials: ME
authors.lastname: Hernández
mesh_terms: D000679:Amygdala / Q000187:drug effects*; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation / Q000009:adverse effects; D004827:Epilepsy / Q000188:drug therapy*; D015918:Infusion Pumps, Implantable; D007696:Kindling, Neurologic / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000927:Anticonvulsants; D005680:gamma-Aminobutyric Acid