title: Single cell RT-PCR demonstrates differential expression of GABAC receptor rho subunits in rat hippocampal pyramidal and granule cells.
abstract: Although gamma-aminobutyric acid (GABA)C receptor rho1, rho2 and rho3 subunits are reportedly expressed in pyramidal and granule cells in the hippocampus at various developmental stages, it is not clear whether these three rho subunits are coexpressed in a single neuron. To attempt to answer this question, we performed single-cell RT-PCR for rho subunits from neurons of rat brain hippocampus. In hippocampal cultures, pyramidal cells were positive for rho1 mRNA expression in 89%, rho2 in 94% and rho3 in 94%, while granule cells were positive for rho1 mRNA in only 6%, rho2 in 36% and rho3 in 91%. Intensive amplification of the RT-PCR products by the second PCR revealed that all the three rho subunits were coexpressed in a single pyramidal and granule cells from both of the cultures and the slices. These results suggest that all the three GABAC receptor rho1, rho2 and rho3 subunits are present probably in different compositions in pyramidal and granule cells in the rat hippocampus.
authors.affiliation: Department of Pharmacology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka 570-8506, Japan.
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.forename: Naoki
authors.initials: N
authors.lastname: Hattori
authors.forename: Baohong
authors.initials: B
authors.lastname: Jiang
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Nakayama
authors.forename: Nan-Yan
authors.initials: NY
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
authors.forename: Kaori
authors.initials: K
authors.lastname: Kitagawa
authors.forename: Megumi
authors.initials: M
authors.lastname: Taketo
authors.forename: Hiroko
authors.initials: H
authors.lastname: Matsuda
authors.forename: Chiyoko
authors.initials: C
authors.lastname: Inagaki
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D066298:In Vitro Techniques; D009434:Neural Pathways / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D021122:Protein Subunits / Q000235:genetics; D017966:Pyramidal Cells / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D018079:Receptors, GABA / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C087200:GABA-C receptor; D021122:Protein Subunits; D012333:RNA, Messenger; D018079:Receptors, GABA