Monitoring the polysaccharide quality of Agaricus blazei in submerged culture by examining molecular weight distribution and TNF-alpha release capability of macrophage cell line RAW 264.7.
A method for monitoring the biological activity of broth polysaccharides of Agaricus blazei (AB-BP) in a submerged culture is described. The TNF-alpha releasing capability of AB-BP on the macrophage cell line, RAW 264.7, correlated with the molecular weight of AB-BP. The quality of polysaccharide in a submerged culture of Agaricus blazei was indirectly monitored by analyzing the distribution of its molecular weight within 1 h. The harvest time of the maximum polysaccharide production did not coincide with that of the maximum biological characteristics in the batch culture.
Shu
Chin-Hang
CH
Department of Chemical and Materials Engineering, National Central University, Chung-Li, Taoyuan, Taiwan 32054. chinshu@cc.ncu.edu.tw
Wen
Bor-Jiun
BJ
Lin
Ko-Jung
KJ
D000364:Agaricus / Q000378:metabolism*; D000818:Animals; D001709:Biotechnology / Q000379:methods*; D002460:Cell Line; D003470:Culture Media; D005285:Fermentation; D007700:Kinetics; D008264:Macrophages / Q000378:metabolism* / Q000382:microbiology; D051379:Mice; D011134:Polysaccharides / Q000737:chemistry*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D003470:Culture Media; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha