HPV-16 L1 genes with inactivated negative RNA elements induce potent immune responses.
Introduction of point mutations in the 5' end of the human papillomavirus type 16 (HPV-16) L1 gene specifically inactivates negative regulatory RNA processing elements. DNA vaccination of C57Bl/6 mice with the mutated L1 gene resulted in improved immunogenicity for both neutralizing antibodies as well as for broad cellular immune responses. Previous reports on the activation of L1 by codon optimization may be explained by inactivation of the regulatory RNA elements. The modified HPV-16 L1 DNA that induced anti-HPV-16 immunity may be seen as a complementary approach to protein subunit immunization against papillomavirus.
Rollman
Erik
E
Department of Virology, Swedish Institute for Infectious Disease Control, Solna, Sweden. erik.rollman@smi.ki.se
Arnheim
Lisen
L
Collier
Brian
B
Oberg
Daniel
D
Hall
Håkan
H
Klingström
Jonas
J
Dillner
Joakim
J
Pastrana
Diana V
DV
Buck
Chris B
CB
Hinkula
Jorma
J
Wahren
Britta
B
Schwartz
Stefan
S
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D036022:Capsid Proteins; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005809:Genes, Regulator; D005814:Genes, Viral; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D009500:Neutralization Tests; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000276:immunology*; D027383:Papillomaviridae / Q000235:genetics / Q000276:immunology*; D030361:Papillomavirus Infections / Q000097:blood / Q000276:immunology* / Q000517:prevention & control; D017354:Point Mutation; D013154:Spleen / Q000276:immunology; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage
D000914:Antibodies, Viral; D036022:Capsid Proteins; D009856:Oncogene Proteins, Viral; D019444:Vaccines, DNA; C063317:L1 protein, Human papillomavirus type 16