Complete primary structure and genomic organization of the mouse Col14a1 gene.
The entire mouse cDNA sequence for type XIV collagen was determined using overlapping PCR products. The 6456 nucleotide (nt) cDNA sequence contains a 5391-nt open reading frame encoding 1797 amino acid residues. The amino terminus has a 28-residue signal peptide that is followed by the mature polypeptide of 1769 amino acid residues with a calculated molecular mass of 193.2 kDa. The mouse alpha1(XIV) collagen chain is predicted to contain all the structural domains described for the polypeptide in chicken and human. These include fibronectin type III repeats, von Willebrand factor A domains, thrombospondin-N-terminal-like domains and two triple-helical domains similar to those of other collagen family members. The amino acid residue sequence of human alpha1(XIV) collagen showed an overall identity of 74% to the chicken sequence and 88% to the human sequence. The entire mouse genomic structure has been determined and is made up of 48 exons. Alternatively spliced forms of mouse type XIV, collagen were not identified corresponding to the findings for the human form.
Gerecke
Donald R
DR
Department of Pharmacology and Toxicology, Environmental and Occupational Health Sciences Institute, Ernest Mario School of Pharmacy, Rutgers University, Piscataway, NJ 08854, USA.
Meng
Xianmin
X
Liu
Bin
B
Birk
David E
DE
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D003094:Collagen / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D029721:Drosophila Proteins; D016678:Genome; D006023:Glycoproteins; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D010711:Phosphatidate Phosphatase; D017434:Protein Structure, Tertiary / Q000235:genetics; D017386:Sequence Homology, Amino Acid
C054447:COL14A1 protein, human; C504563:Col14a1 protein, mouse; D018076:DNA, Complementary; D029721:Drosophila Proteins; D006023:Glycoproteins; D003094:Collagen; D010711:Phosphatidate Phosphatase; C492536:Wun2 protein, Drosophila