Ability of Escherichia coli O157:H7 isolates to colonize the intestinal tract of conventional adult CD1 mice is transient.
In an attempt to improve upon a current mouse model of intestinal colonization by Escherichia coli O157:H7 used in this laboratory for vaccine development, nine clinical isolates of the pathogen were screened for their ability to persist in the intestinal tract of conventional adult CD-1 mice. None of the test isolates of E. coli O157:H7 were capable of colonizing these mice for a period of more than two weeks. Most of the isolates appeared to be benign for the experimental host, but one isolate was lethal. This virulence correlated with the ability of the latter isolate to produce large quantities of Shiga-like toxin 2 in vitro.
Conlan
J W
JW
National Research Council Canada, Institute for Biological Sciences, Ottawa, ON K1A 0R6, Canada. wayne.conlan@nrc.ca
Bardy
S L
SL
KuoLee
R
R
Webb
A
A
Perry
M B
MB
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood; D001427:Bacterial Toxins / Q000096:biosynthesis / Q000633:toxicity; D015169:Colony Count, Microbial; D004927:Escherichia coli Infections / Q000276:immunology / Q000382:microbiology; D019453:Escherichia coli O157 / Q000254:growth & development* / Q000276:immunology / Q000378:metabolism / Q000472:pathogenicity; D005260:Female; D007070:Immunoglobulin A / Q000032:analysis / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007422:Intestines / Q000382:microbiology*; D051379:Mice; D022641:Shiga Toxin 2 / Q000096:biosynthesis / Q000633:toxicity; D013997:Time Factors; D014774:Virulence
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D022641:Shiga Toxin 2