Immunisation against PCV2 structural protein by DNA vaccination of mice.
Porcine circovirus type 2 (PCV2) is the causative agent of an emerging swine disease, postweaning multisystemic wasting syndrome (PMWS). The disease affects primarily 5-12-weeks-old pigs which might suggest that infection with PCV2 occurs when the level of maternal antibodies have declined to sub-protective levels around weaning at 3-5-weeks of age. If immunoprophylaxis is to be effective, an immunisation method capable of breaking through maternal immunity must be employed. In this study, we have developed and investigated the potential of a DNA vaccination approach to be one such method. The gene encoding the capsid protein of PCV2 was cloned in a DNA vaccination plasmid and expression of capsid protein was demonstrated in vitro. Mice were gene gun vaccinated three timesand all mice responded serologically by raising antibodies against PCV2. The results suggest, that DNA based vaccination might offer opportunities for vaccination of piglets against PCV2.
Kamstrup
Søren
S
Danish Institute for Food and Veterinary Research, Lindholm, DK-4771 Kalvehave, Denmark. sk@dfvf.dk
Barfoed
Annette Malene
AM
Frimann
Tine Holland
TH
Ladekjaer-Mikkelsen
Anne-Sofie
AS
Bøtner
Anette
A
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis; D018173:Circoviridae Infections / Q000276:immunology* / Q000517:prevention & control*; D017925:Circovirus / Q000235:genetics / Q000276:immunology*; D004279:DNA, Viral / Q000276:immunology; D005260:Female; D005814:Genes, Viral / Q000235:genetics; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D016366:Open Reading Frames / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000821:virology; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology; D015678:Viral Structural Proteins / Q000235:genetics; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D004279:DNA, Viral; D019444:Vaccines, DNA; D015678:Viral Structural Proteins; D014765:Viral Vaccines