Collection of peripheral progenitor cells: a comparison between Amicus and Cobe-Spectra blood cell separators.
The authors compared the efficiency of two different blood cell separators (Amicus and Cobe-Spectra) in collecting peripheral blood progenitor cells for autologous or homologous transplantation. A total number of 129 procedures were performed, 36 with Spectra, 93 with Amicus. There was no difference between Spectra and Amicus efficiencies for CD34+ cell collection (46.685% vs 46.235%; p=n.s) but the platelet efficiencies were 17.31% and 12.54% respectively (p=0.04) and, if autologous and allogeneic collections were considered separately, a marked difference resulted in allogeneic platelet efficiency between 6 Spectra and 23 Amicus procedures (26.83% vs 8.68%, p=0.0004). The authors were able to demonstrate that in 70 Amicus autologous collections there was a different platelet efficiency, if peripheral count was considered: 12 procedures performed with a platelet count > 100 x 10(9)/l had a very low efficiency (6.86%), but this value increased if platelet count lowered (13.02% if between 100 and 50 x 10(9)/l, 22.63% if between 50 and 0 x 10(9)/l, 23 and 35 procedures respectively). The study is preliminary and the number of collections is little, but the overall data suggest that Spectra (AutoPBSC, V 6.0) and Amicus separators have the same efficiency for collecting CD34+ cells while Amicus procedures have a very low platelet contamination, especially with donors.
Adorno
Gaspare
G
Department of Immunohematology, Tor Vergata University of Rome, Viale Oxford, 81, 00133 Rome, Italy. medtrasf@libero.it
Del Proposto
Gianpaolo
G
Palombi
Francesca
F
Bruno
Antonio
A
Ballatore
Giovanna
G
Postorino
Massimiliano
M
Tendas
Andrea
A
Del Poeta
Giovanni
G
Isacchi
Giancarlo
G
Amadori
Sergio
S
D018952:Antigens, CD34 / Q000096:biosynthesis; D000970:Antineoplastic Agents / Q000627:therapeutic use; D001781:Blood Component Removal / Q000295:instrumentation / Q000379:methods*; D001792:Blood Platelets / Q000166:cytology; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods*; D006689:Hodgkin Disease / Q000188:drug therapy; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy; D008228:Lymphoma, Non-Hodgkin / Q000188:drug therapy; D009101:Multiple Myeloma / Q000188:drug therapy; D010976:Platelet Count; D010983:Plateletpheresis; D013234:Stem Cells / Q000276:immunology*; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
D018952:Antigens, CD34; D000970:Antineoplastic Agents