A simple in vitro test tube method for estimating the bioavailability of phosphorus in feed ingredients for swine.
A simplified in vitro test tube (TT) method was developed to estimate the percentage of available P in feed ingredients for swine. The entire digestion procedure with the TT method consists of three consecutive enzymatic digestions carried out in a 50-mL conical test tube: (1) Pre-digestion with endo-xylanase and beta-glucanase for 1 h, (2) peptic digestion for 2 h, and (3) pancreatic digestion for 2 or 4 h. The TT method is simpler and much easier to perform compared to the dialysis tubing (DT) method, because dialysis tubing is not used. Reducing sample size from 1.0 to 0.25 g for the TT method improved results. In conclusion, the accuracy and validity of the TT method is equal to that of our more complicated DT method (r = 0.97, P < 0.001), designed to mimic the digestive system of swine, for estimating the availability of P in plant-origin feed ingredients.
Bollinger
David W
DW
Department of Animal Science, University of Missouri, Columbia, Missouri 65211-5300, USA.
Tsunoda
Atsushi
A
Ledoux
David R
DR
Ellersieck
Mark R
MR
Veum
Trygve L
TL
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D001682:Biological Availability; D010194:Pancreatin / Q000378:metabolism; D010434:Pepsin A / Q000378:metabolism; D016226:Phosphorus, Dietary / Q000493:pharmacokinetics*; D013025:Glycine max / Q000737:chemistry / Q000378:metabolism; D013552:Swine
D016226:Phosphorus, Dietary; D010194:Pancreatin; D010434:Pepsin A