title: Simultaneous determination of histamine and histidine by liquid chromatography following intramolecular excimer-forming fluorescence derivatization with pyrene-labeling reagent.
abstract: A highly sensitive and selective fluorometric method for the determination of histamine and histidine has been developed. This method is based on an intramolecular excimer-forming fluorescence derivatization with a pyrene reagent followed by reversed-phase liquid chromatography. The analytes, containing two amino moieties in a molecule, were converted to the corresponding dipyrene-labeled derivatives by derivatization. The derivatives afforded intramolecular excimer fluorescence (440 - 540 nm), which can clearly be discriminated from the normal fluorescence (360 - 420 nm) emitted from reagent blanks. The detection limits (signal-to-noise ratio = 3) were femto mole levels.
authors.affiliation: Faculty of Pharmaceutical Sciences, Fukuoka University, Johnan, Fukuoka 814-0180, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Yoshida
authors.forename: Fumio
authors.initials: F
authors.lastname: Ichinose
authors.forename: Takashi
authors.initials: T
authors.lastname: Yoshitake
authors.forename: Yukitaka
authors.initials: Y
authors.lastname: Nakano
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Todoroki
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nohta
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Yamaguchi
mesh_terms: D002138:Calibration; D002853:Chromatography, Liquid; D005456:Fluorescent Dyes; D006632:Histamine / Q000032:analysis*; D006639:Histidine / Q000032:analysis*; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D011721:Pyrenes / Q000737:chemistry; D012015:Reference Standards; D012996:Solutions; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D011721:Pyrenes; D012996:Solutions; D006639:Histidine; D006632:Histamine