The prothrombin 3'end formation signal reveals a unique architecture that is sensitive to thrombophilic gain-of-function mutations.
The functional analysis of the common prothrombin 20210 G>A(F2 20210(*)A) mutation has recently revealed gain of function of 3'end processing as a novel genetic mechanism predisposing to human disease. We now show that the physiologic G at the cleavage site at position 20210 is the functionally least efficient nucleotide to support 3'end processing but has evolved to be physiologically optimal. Furthermore, the F2 3'end processing signal is characterized by a weak downstream cleavage stimulating factor (CstF) binding site with a low uridine density, and the functional efficiency of F2 3'end processing can be enhanced by the introduction of additional uridine residues. The recently identified thrombosis-related mutation (F2 20221(*)T) within the CstF binding site up-regulates F2 3'end processing and prothrombin biosynthesis in vivo. F2 20221(*)T thus represents the first example of a likely pathologically relevant mutation of the putative CstF binding site in the 3'flanking sequence of a human gene. Finally, we show that the low-efficiency F2 cleavage and CstF binding sites are balanced by a stimulatory upstream uridine-rich element in the 3'UTR. The architecture of the F2 3'end processing signal is thus characterized by a delicate balance of positive and negative signals. This balance appears to be highly susceptible to being disturbed by clinically relevant gain-of-function mutations.
Danckwardt
Sven
S
Molecular Medicine Partnership Unit, Im Neuenheimer Feld 153, 69120 Heidelberg, Germany.
Gehring
Niels H
NH
Neu-Yilik
Gabriele
G
Hundsdoerfer
Patrick
P
Pforsich
Margit
M
Frede
Ute
U
Hentze
Matthias W
MW
Kulozik
Andreas E
AE
D020413:3' Untranslated Regions / Q000235:genetics*; D006502:Budd-Chiari Syndrome / Q000235:genetics; D006528:Carcinoma, Hepatocellular; D006367:HeLa Cells; D006801:Humans; D010641:Phenotype; D017354:Point Mutation; D017434:Protein Structure, Tertiary; D011516:Prothrombin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D013927:Thrombosis / Q000503:physiopathology*; D014529:Uridine / Q000235:genetics
D020413:3' Untranslated Regions; D011516:Prothrombin; D014529:Uridine