title: Identification of Chlamydophila pneumoniae in an emerald tree boa, Corallus caninus.
abstract: Tissues were evaluated from emerald tree boas, Corallus caninus, from a collection in which chlamydiosis was diagnosed. To determine the strain of chlamydia infecting these snakes, tissue samples from 5 frozen snakes were tested by a quantitative TaqMan polymerase chain reaction (PCR) test and a PCR sequence analysis test. Of the 22 samples tested, 9 were categorized as either positive or weakly positive with the TaqMan test, and 6 yielded an amplicon using a serial PCR test that amplified a portion of the 23S ribosomal RNA gene. A PCR product suitable for sequencing was obtained from the heart of one of the snakes. Sequence analysis showed that the snake had been infected with Chlamydophila pneumoniae. These findings show that C. pneumoniae can infect emerald tree boas, broadening the range of reptiles known to be infected by this primarily human pathogen.
authors.affiliation: Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Elliott R
authors.initials: ER
authors.lastname: Jacobson
authors.forename: Darryl
authors.initials: D
authors.lastname: Heard
authors.forename: Arthur
authors.initials: A
authors.lastname: Andersen
mesh_terms: D000818:Animals; D017821:Boidae / Q000382:microbiology*; D023521:Chlamydophila Infections / Q000382:microbiology / Q000662:veterinary*; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000254:growth & development*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012338:RNA, Ribosomal, 23S / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012338:RNA, Ribosomal, 23S