Development of protamine hollow-fiber device for extracorporeal heparin removal.
Protamine was covalently coupled to a cellulose hollow fiber (cuprophan membrane) through epichlorohydrin-activated hydroxyl groups on the hollow fiber. An immobilization efficiency of as much as 15 mg protamine/g fiber could be achieved under optimal reacting conditions. Compared to a protamine hollow-fiber device produced using cyanogen bromide as an activator, the protamine hollow-fiber device using epichlorohydrin as an activator shows very high resistance to hydrolysis. Both in vitro and in vivo experiments showed that such a protamine hollow-fiber device had high heparin-removal capacity and efficiency, suggesting that this device may be suitable for various clinical applications in extracorporeal heparin removal.
Liang
Jun F
JF
Department of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, Ann Arbor, Mich. 48109-1065, USA. junfeng@umich.edu
Emregul
Emel
E
Yang
Victor C
VC
D000327:Adsorption; D000818:Animals; D020099:Coated Materials, Biocompatible / Q000627:therapeutic use*; D016903:Drug Monitoring; D004811:Epichlorohydrin; D004867:Equipment Design; D005112:Extracorporeal Circulation / Q000379:methods*; D006470:Hemorrhage / Q000517:prevention & control; D006493:Heparin / Q000009:adverse effects / Q000097:blood* / Q000302:isolation & purification; D006494:Heparin Antagonists / Q000627:therapeutic use*; D006801:Humans; D006868:Hydrolysis; D011479:Protamines / Q000627:therapeutic use*; D051381:Rats
D020099:Coated Materials, Biocompatible; D006494:Heparin Antagonists; D011479:Protamines; D004811:Epichlorohydrin; D006493:Heparin