Cytoplasm prepared from all-trans retinoic acid (ATRA)-treated human hematopoietic progenitor cells induces differentiation of both ATRA sensitive and ATRA resistant leukemia cells.
In this study, hematopoietic cells from human placental blood were treated with the differentiation-inducing drug all-trans retinoic acid (ATRA). Their cytoplasm was then used to culture the human myelogenous leukemia cell line HL60, NB(4) cells and the RA-resistant HL60-R and NB(4)-R(2) cells. All of these four kinds of leukemia cells underwent macrophage/monocyte differentiation and apoptosis, while their proliferation was inhibited. This suggests that the RA receptors were not essentially needed in this situation, and this method should be applicable in the treatment of RA-resistant promyelocytic leukemia as well as other kinds of leukemia.
Zhang
Hongjun
H
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100-084, China.
Wang
Zhao
Z
Zhou
Jiangbing
J
D017209:Apoptosis / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D003470:Culture Media; D003593:Cytoplasm / Q000187:drug effects; D019008:Drug Resistance, Neoplasm; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007938:Leukemia; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D010920:Placenta / Q000098:blood supply; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D003470:Culture Media; D014212:Tretinoin