Cytoplasmic alkalization precedes reactive oxygen species production during methyl jasmonate- and abscisic acid-induced stomatal closure.
Signaling events during abscisic acid (ABA) or methyl jasmonate (MJ)-induced stomatal closure were examined in Arabidopsis wild type, ABA-insensitive (ost1-2), and MJ-insensitive mutants (jar1-1) in order to examine a crosstalk between ABA and MJ signal transduction. Some of the experiments were performed on epidermal strips of Pisum sativum. Stomata of jar1-1 mutant plants are insensitive to MJ but are able to close in response to ABA. However, their sensitivity to ABA is less than that of wild-type plants. Reciprocally, the stomata of ost1-2 are insensitive to ABA but are able to close in response to MJ to a lesser extent compared to wild-type plants. Both MJ and ABA promote H(2)O(2) production in wild-type guard cells, while exogenous application of diphenylene iodonium (DPI) chloride, an inhibitor of NAD(P)H oxidases, results in the suppression of ABA- and MJ-induced stomatal closure. ABA elevates H(2)O(2) production in wild-type and jar1-1 guard cells but not in ost1-2, whereas MJ induces H(2)O(2) production in both wild-type and ost1-2 guard cells, but not in jar1-1. MJ-induced stomatal closing is suppressed in the NAD(P)H oxidase double mutant atrbohD/F and in the outward potassium channel mutant gork1. Furthermore, MJ induces alkalization in guard cell cytosol, and MJ-induced stomatal closing is inhibited by butyrate. Analyses of the kinetics of cytosolic pH changes and reactive oxygen species (ROS) production show that the alkalization of cytoplasm precedes ROS production during the stomatal response to both ABA and MJ. Our results further indicate that JAR1, as OST1, functions upstream of ROS produced by NAD(P)H oxidases and that the cytoplasmic alkalization precedes ROS production during MJ or ABA signal transduction in guard cells.
Suhita
Dontamala
D
CEA/Cadarache-DSV-DEVM, Laboratoire des Echanges Membranaires et Signalisation, UMR 163 CNRS-CEA, Université de la Méditerranée, 13108 St Paul lez Durance cedex, France.
Raghavendra
Agepati S
AS
Kwak
June M
JM
Vavasseur
Alain
A
D000040:Abscisic Acid / Q000494:pharmacology*; D000085:Acetates / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D002147:Calmodulin / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology*; D003593:Cytoplasm / Q000737:chemistry / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D009154:Mutation; D009247:NADH, NADPH Oxidoreductases / Q000187:drug effects / Q000378:metabolism; D019255:NADPH Oxidases; D054883:Oxylipins; D018532:Pisum sativum / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D019441:Plant Epidermis / Q000187:drug effects / Q000502:physiology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D011494:Protein Kinases / Q000187:drug effects / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology
D000085:Acetates; D002147:Calmodulin; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D017382:Reactive Oxygen Species; D000040:Abscisic Acid; C072239:methyl jasmonate; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases; D011494:Protein Kinases; D002118:Calcium