[Characterization of human lung cancer cell line A549 transfected with human interferon-gamma gene].
To establish a human lung cancer cell line expressing human interferon-gamma.



The full-length gene of human interferon-gamma (IFN-gamma) was introduced into the human lung adenocarcinoma cell line A549 through retroviral vector pLXSN. The established cell line A549-IFN-gamma was tested for expression of MHC class I and class II by flow cytometer (FCM) and tested for expression of IFN-gamma by enzyme-lined immunoadsorbent assay (ELISA ). The tumorigenesis of cell line A549-IFN-gamma was tested on nude mice.



A high level of IFN-gamma protein was detected in the culture supernatants of cell line A549-IFN-gamma. The expressions of MHC class I and class II on A549-IFN-gamma cells increased significantly (P<0.01), when compared with parental cell line A549. However, there was no significant difference (P<0.05) between the growth of cell line A549-IFN-gamma and A549. Finally, the tumorigenesis test showed that A549-IFN-gamma had lower tumorigenetic effects than A549.



The results indicate that introduction of human IFN-gamma gene into cell line A549 could increase its immunogenicity and decrease its tumorigenesis. With the established cell line A549-IFN-gamma, a tumor vaccine for human lung cancer may be developed.
Yu
Xiao-hong
XH
College of Medicine Zhejinag University, Hangzhou 310031, China. xiaohyu65@163.com
Liu
Xiang-lin
XL
Ma
Jie-yu
JY
D000818:Animals; D019496:Cancer Vaccines / Q000276:immunology; D045744:Cell Line, Tumor; D015316:Genetic Therapy; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D008175:Lung Neoplasms / Q000276:immunology / Q000628:therapy*; D008297:Male; D051379:Mice; D014162:Transfection
D019496:Cancer Vaccines; D007371:Interferon-gamma