Identification of a new hobo element in the cabbage moth, Mamestra brassicae (Lepidoptera).
A complete hobo-like element, called Mbhobo, was identified in the cabbage moth, Mamestra brassicae. This element has a high sequence similarity to the HFL1 hobo element of Drosophila melanogaster. Amplification of Mbhobo termini indicated that transposition occurred into a 5'-GTGGGTAC-3' target sequence that was duplicated upon insertion. This target site conforms to the consensus sequence established for the insertion sites of insect hAT elements. Mbhobo has a single 1935 bp long ORF with significant homology to the D. melanogaster HFL1 hobo transposase. FISH experiments evidenced Mbhobo clusters located in heterochromatic regions of Z and W sex chromosomes and in heterochromatic areas of chromosome pair 10.
Borsatti
Federica
F
Dipartimento di Biologia Animale, Università di Modena e Reggio Emilia, Modena, Italy.
Azzoni
Paola
P
Mandrioli
Mauro
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA; D004251:DNA Transposable Elements / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007915:Lepidoptera / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames
D004251:DNA Transposable Elements; D004247:DNA