Morphology of auditory hair cells in guinea pig cochlea after transgene expression.
It is very important to determine if recombinant adenoviral vector (Ad) can damage the auditory hair cells in guinea pig cochlea after transgene expression. In this study, the scanning electron microscope was used to determine if there was loss of the auditory hair cells after Ad.LacZ (Ad5 containing Escherichia coli galactosidase) was inoculated into the cochlea through the round window membrane. Seven days later all inner and outer hair cells were found to express the LacZ gene. Except for the sparse loss of outer hair cells in the basal turn and the second turn, there was insignificant loss in the other turns. All inner hair cells were present. The damage to auditory hair cells resulting from intracochlear inoculation of Ad is limited, and this vector can be used as one of the ideal delivery tools in gene therapy of the cochlea.
Han
Demin
D
Department of Otorhinolaryngology, Affiliated Bejing Tongren Hospital of Capital University of Medical Sciences, Beijing, PR China.
Yu
Zilong
Z
Fan
Erzhong
E
Liu
Chan
C
Liu
Sha
S
Li
Ying
Y
Liu
Zhongyan
Z
D000256:Adenoviridae / Q000235:genetics / Q000502:physiology; D000818:Animals; D016022:Case-Control Studies; D003051:Cochlea / Q000648:ultrastructure; D005786:Gene Expression Regulation; D015316:Genetic Therapy / Q000009:adverse effects / Q000379:methods; D005822:Genetic Vectors / Q000009:adverse effects*; D006168:Guinea Pigs; D006199:Hair Cells, Auditory, Inner / Q000033:anatomy & histology / Q000648:ultrastructure*; D018072:Hair Cells, Auditory, Outer / Q000033:anatomy & histology / Q000648:ultrastructure*; D007150:Immunohistochemistry; D007763:Lac Operon / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D009925:Organ of Corti / Q000648:ultrastructure; D019076:Transgenes / Q000502:physiology