Modified colorimetric assay for susceptibility testing of azole antifungal drugs against Candida species.
We modified a rapid susceptibility assay (RSA) for antifungal susceptibility testing of azoles based upon glucose utilization. This modified RSA method provides quantitative endpoint readings in 6 h. In this study, the modified RSA and the National Committee for Clinical Laboratory Standards M27-A methods were used to determine the MICs of fluconazole and itraconazole for 118 Candida isolates. Yeast nitrogen base containing 0.12 g of glucose per liter was used for the modified RSA method. For fluconazole, agreement among assays within one or two twofold dilutions was 72.9 and 88.1%, respectively; for itraconazole, agreement within one or two twofold dilutions was 82.2 and 89.8%, respectively. These data suggest that the modified RSA method is a reliable and rapid method for azole antifungal susceptibility testing against Candida species.
Chen
Jian
J
Research Center for Medical Mycology, Peking University, Beijing, People's Republic of China.
Wan
Zhe
Z
Li
Ruoyu
R
D000935:Antifungal Agents / Q000494:pharmacology; D001393:Azoles / Q000494:pharmacology*; D002175:Candida / Q000145:classification / Q000187:drug effects* / Q000378:metabolism; D003124:Colorimetry; D003470:Culture Media; D015725:Fluconazole / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D017964:Itraconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards
D000935:Antifungal Agents; D001393:Azoles; D003470:Culture Media; D017964:Itraconazole; D015725:Fluconazole; D005947:Glucose