title: Production of L-phenylacetyl carbinol by immobilized cells of Saccharomyces cerevisiae.
abstract: Conversion of benzaldehyde to L-phenylacetyl carbinol (L-PAC) was achieved with immobilized, growing cells of Saccharomyces cerevisiae in different reactors. Product formation increased (31%) with the subsequent initial reuses of the entrapped cells. Biomass production and PAC formation depleted (40 and 57%, respectively) after 4-5 continuous growth and biotransformation cycles. With the regeneration of the biocatalysts, catalytic activity of the cells was resumed. The highest yields were in a stirred tank reactor (29 g PAC) from 77 g benzeldehyde with 14 repeated uses of entrapped cells.
authors.affiliation: Division of Fermentation Technology, Central Drug Research Institute, Lucknow 226 001, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Mandwal
authors.forename: C K M
authors.initials: CK
authors.lastname: Tripathi
authors.forename: P D
authors.initials: PD
authors.lastname: Trivedi
authors.forename: A K
authors.initials: AK
authors.lastname: Joshi
authors.forename: S C
authors.initials: SC
authors.lastname: Agarwal
authors.forename: Vinod
authors.initials: V
authors.lastname: Bihari
mesh_terms: D000096:Acetone / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism*; D001547:Benzaldehydes / Q000378:metabolism*; D019149:Bioreactors / Q000145:classification / Q000382:microbiology*; D001711:Biotransformation; D002384:Catalysis; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D018914:Cells, Immobilized / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D001547:Benzaldehydes; D000096:Acetone; C035293:1-hydroxy-1-phenyl-2-propanone; C032175:benzaldehyde