title: Comparison of real-time PCR and EIA for the detection of outbreaks of acute gastroenteritis caused by norovirus.
abstract: We compared real-time polymerase chain reaction (PCR) and a newly developed enzyme immunoassay (EIA) for the detection of noroviruses (NoV) in 70 stool samples taken from outbreaks and sporadic cases of acute gastroenteritis. The real-time PCR classified more samples as positive than the EIA (26 versus 10) and detected more outbreaks of NoV (six versus two). Real-time PCR offers rapid and sensitive diagnosis for both sporadic cases and outbreaks of NoV gastroenteritis and, by comparison, the reduced sensitivity and specificity of the EIA will result in negative samples having to be tested by PCR. This delay might undermine current infection control procedures, lead to further dissemination of infection and increase healthcare costs.
authors.affiliation: West of Scotland Specialist Virology Centre, Gartnavel General Hospital, Great Western Road, Glasgow G12 0YN. rory.gunson@northglasgow.scot.nhs.uk
authors.forename: R N
authors.initials: RN
authors.lastname: Gunson
authors.forename: J
authors.initials: J
authors.lastname: Miller
authors.forename: W F
authors.initials: WF
authors.lastname: Carman
mesh_terms: D000208:Acute Disease; D001483:Base Sequence; D017250:Caliciviridae Infections / Q000453:epidemiology / Q000821:virology*; D017931:DNA Primers; D004196:Disease Outbreaks; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D029322:Norovirus / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012606:Scotland / Q000453:epidemiology; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers