Cardiac sympathetic afferent stimulation impairs baroreflex control of renal sympathetic nerve activity in rats.
It is well known that cardiac sympathetic afferent reflexes contribute to increases in sympathetic outflow and that sympathetic activity can antagonize arterial baroreflex function. In this study, we tested the hypothesis that in normal rats, chemical and electrical stimulation of cardiac sympathetic afferents results in a decrease in the arterial baroreflex function by increasing sympathetic nerve activity. Under alpha-chloralose (40 mg/kg) and urethane (800 mg/kg i.p.) anesthesia, renal sympathetic nerve activity, mean arterial pressure, and heart rate were recorded. The arterial baroreceptor reflex was evaluated by infusion of nitroglycerin (25 microg i.v.) and phenylephrine (10 microg i.v.). Left ventricular epicardial application of capsaicin (0.4 microg in 2 microl) blunted arterial baroreflex function by 46% (maximum slope 3.5 +/- 0.3 to 1.9 +/- 0.2%/mmHg, P < 0.01). When the central end of the left cardiac sympathetic nerve was electrically stimulated (7 V, 1 ms, 20 Hz), the sensitivity of the arterial baroreflex was similarly decreased by 42% (maximum slope 3.2 +/- 0.3 to 1.9 +/- 0.4%/mmHg; P < 0.05). Pretreatment with intracerebroventricular injection of losartan (500 nmol in 1 microl of artificial cerebrospinal fluid) completely prevented the impairment of arterial baroreflex function induced by electrical stimulation of the central end of the left cardiac sympathetic nerve (maximum slope 3.6 +/- 0.4 to 3.1 +/- 0.5%/mmHg). These results suggest that the both chemical and electrical stimulation of the cardiac sympathetic afferents reduces arterial baroreflex sensitivity and the impairment of arterial baroreflex function induced by cardiac sympathetic afferent stimulation is mediated by central angiotensin type 1 receptors.
Gao
Lie
L
Department of Physiology and Biophysics, University of Nebraska Medical Center, University of Nebraska College of Medicine, Omaha, Nebraska 68198-4575, USA.
Zhu
Zhen
Z
Zucker
Irving H
IH
Wang
Wei
W
D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage; D001158:Arteries / Q000294:innervation; D017704:Baroreflex / Q000187:drug effects / Q000502:physiology*; D002211:Capsaicin / Q000494:pharmacology; D004558:Electric Stimulation; D006321:Heart / Q000294:innervation*; D007276:Injections, Intraventricular; D007668:Kidney / Q000294:innervation*; D019808:Losartan / Q000008:administration & dosage; D008297:Male; D009475:Neurons, Afferent / Q000502:physiology*; D010496:Pericardium / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013268:Stimulation, Chemical; D013564:Sympathetic Nervous System / Q000502:physiology*
D000959:Antihypertensive Agents; D019808:Losartan; D002211:Capsaicin