The cytotoxic activity of the total alkaloids isolated from different parts of Solanum pseudocapsicum.
The total alkaloid fractions of the methanolic extracts of the leaves, ripe fruits, roots, seeds and stem of Solanum pseudocapsicum were subjected to in-vitro cytotoxicity, short-term toxicity and long-term survival studies. All the five fractions exhibited potent activity. The total alkaloid fraction of leaves was found to be the most potent. The HT-29 cell line was the most sensitive to the fractions. The cytotoxic concentration (CTC(50)) values for all these fractions ranged between 0.39-0.91, 0.68-2.8, 0.92-3.56, 4.05-8.2, 3.28-5.65 and 0.95-5.55 microg/ml, respectively for HT-29, RD-228, A-549, HEp-2, B(16)F(10) and Vero cell lines. In short-term toxicity studies, the fractions showed 50% viability at 93-128 microg/ml for DLA cells and 141-189 microg/ml for human lymphocytes. In the long-term survival studies on the cell lines RD-228, HEp-2 and Vero, cells retained their regenerative capacities at concentrations below 8 microg/ml. The total alkaloids of the plant, especially from the leaves merit further investigations to identify the active constituents in animal models.
Vijayan
Pottekkad
P
J.S.S. College of Pharmacy, Rocklands, OOTACAMUND-643 001, Tamil Nadu, India. vijayanp4@rediffmail.com
Vijayaraj
Preethi
P
Setty
Prashanth Haranahalli Chandrappa
PH
Hariharpura
Raghu Chandrashekhar
RC
Godavarthi
Ashok
A
Badami
Shrishailappa
S
Arumugam
Dhanaraj Sokkalingam
DS
Bhojraj
Suresh
S
D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology* / Q000633:toxicity; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology; D018514:Plant Structures / Q000737:chemistry; D019657:Solanaceae / Q000737:chemistry*
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts