title: Enhanced bactericidal action and masking of allergen structure of soy protein by attachment of chitosan through maillard-type protein-polysaccharide conjugation.
abstract: The soy protein-chitosan conjugate was formed by the Maillard reaction in dry state (relative humidity 65%) at 60 degrees C for 2 weeks to improve the functional properties. The antimicrobial activity of the Maillard-type soy protein-chitosan conjugates enhanced 2-3 times that of soy protein-chitosan mixture. The soy protein-chitosan conjugate showed excellent emulsifying property with the progress of Maillard-type conjugation. The allergenicity of soy protein was greatly decreased by the attachment of chitosan through Maillard reaction. The immonoblotting analysis with patient's sera revealed that soy protein-chitosan conjugate was more effective to mask the allergen structure of soy protein causing from 34 kDa-protein (Gly m Bd 30K) than soy protein-galactomannan conjugate. The Western blotting showed that allergen (34 kDa-protein) was completely masked by soy protein-chitosan conjugation, while it was not completely masked by soy protein-galactomannan conjugation.
authors.affiliation: Department of Bioloical Chemistry, Faculty of Agricultural Science, Yamaguchi University, Yamaguchi, Japan.
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Usui
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tamura
authors.forename: Kimiko
authors.initials: K
authors.lastname: Nakamura
authors.forename: Tadashi
authors.initials: T
authors.lastname: Ogawa
authors.forename: Mayumi
authors.initials: M
authors.lastname: Muroshita
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Azakami
authors.forename: Shin
authors.initials: S
authors.lastname: Kanuma
authors.forename: Akio
authors.initials: A
authors.lastname: Kato
mesh_terms: D000485:Allergens / Q000032:analysis* / Q000276:immunology; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry*; D048271:Chitosan; D004591:Electrophoresis, Polyacrylamide Gel; D004655:Emulsions; D006801:Humans; D006813:Humidity; D015151:Immunoblotting; D015416:Maillard Reaction; D008970:Molecular Weight; D030262:Soybean Proteins / Q000737:chemistry* / Q000276:immunology
chemical_list: D000485:Allergens; D000900:Anti-Bacterial Agents; D004655:Emulsions; D030262:Soybean Proteins; D002686:Chitin; D048271:Chitosan