Co-culture of hepatocytes and fibroblasts by micropatterned immobilization of beta-galactose derivatives.
Photosensitive poly(allylamine) containing beta-galactose moieties in the side chain (LPAN(3)) was prepared by the reaction of poly(allylamine) with lactobionic acid and azidobenzoic acid. To create micropatterned surfaces, a LPAN(3)-coated poly(methyl methacrylate) (PMMA) substrate was irradiated with an ultraviolet lamp under a photomask. The presence of a LPAN(3) layer on the substrate was confirmed using an electron spectroscopy for chemical analysis. Micropatterned cell culture was carried out by seeding hepatocytes and/or fibroblasts on the substrate. As a result, hepatocytes and fibroblasts adhered only to the LPAN(3) and PMMA lane, respectively. Co-culture on the stripe patterned substrate was carried out by the first cell seeding of hepatocytes and subsequently by the second cell seeding of fibroblasts. The co-cultured cells produced extracellular matrix such as fibronectin, suggesting a biological function.
Kang
Inn-Kyu
IK
Department of Polymer Science, Kyungpook National University, Puk-gu, Taegu 702-701, South Korea. ikkang@bh.kyungppok.ac.kr
Kim
Geel Ja
GJ
Kwon
Oh Hyeong
OH
Ito
Yoshihiro
Y
D016475:3T3 Cells; D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D018920:Coculture Techniques / Q000379:methods*; D005347:Fibroblasts / Q000166:cytology*; D005690:Galactose / Q000737:chemistry*; D022781:Hepatocytes / Q000166:cytology*; D008297:Male; D008422:Materials Testing; D051379:Mice; D011073:Polyamines / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D051381:Rats; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D011073:Polyamines; C063994:polyallylamine; D019904:Polymethyl Methacrylate; D005690:Galactose