title: Differential development of 5-HT receptor and the serotonin transporter binding in the human infant medulla.
abstract: Tissue receptor autoradiography with 3H-lysergic acid diethylamide (3H-LSD), 3H-8-hydroxy-2-[di-N-propylamine] tetralin (3H-8-OH-DPAT), and 125I-RTI-55 was used to map the distribution and developmental profile of 5-HT(1A-1D) and 5-HT2 receptors, 5-HT1A receptors, and the serotonin (5-HT) transporter (SERT), respectively, to nuclei with cardiorespiratory function in the human medulla from midgestation to maturity. The distribution pattern of the 5-HT markers was heterogeneous, with variable densities of binding of each observed both in nuclei with and without 5-HT cell bodies. The highest density of binding for each marker was observed in the raphé nuclei, the site of the highest density of 5-HT cell bodies. A significant reduction in 5-HT receptor binding measured with 3H-LSD was observed between midgestation and infancy, and between infancy and maturity in multiple nuclei, but no changes were observed across infancy. A significant increase in 5-HT1A receptor binding density was observed across infancy in the hypoglossal nucleus (regression slope coefficient = 0.008 +/- 0.002, P = 0.02), and a marginally significant increase was observed in the raphé obscurus (regression slope coefficient = 0.061 +/- 0.026 [mean +/- SEM], P = 0.05). No significant age-related changes in SERT binding were observed at any time. With the exception of the hypoglossal nucleus, where 5-HT1A receptor binding increases while SERT binding remains stable, the medullary 5-HT markers analyzed in the study are essentially "in place" at birth. This study provides important baseline data that serve as a foundation for future work in pediatric 5-HT brainstem disorders, including sudden infant death syndrome.
authors.affiliation: Department of Neurology, Children's Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA. david.paterson@tch.harvard.edu
authors.forename: David S
authors.initials: DS
authors.lastname: Paterson
authors.forename: Richard A
authors.initials: RA
authors.lastname: Belliveau
authors.forename: Felicia
authors.initials: F
authors.lastname: Trachtenberg
authors.forename: Hannah C
authors.initials: HC
authors.lastname: Kinney
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D005333:Fetus / Q000378:metabolism; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008526:Medulla Oblongata / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D011985:Receptors, Serotonin / Q000378:metabolism*; D018490:Serotonin Agents / Q000378:metabolism; D050486:Serotonin Plasma Membrane Transport Proteins
chemical_list: D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D011985:Receptors, Serotonin; C493230:SLC6A4 protein, human; D018490:Serotonin Agents; D050486:Serotonin Plasma Membrane Transport Proteins