Methyl and phenyl esters and thioesters of carboxylic acids as surrogate substrates for microassay of proteinase K esterase activity.
The development of a microassay for proteinase K esterase activity with carboxylic acid esters is reported using novel substrates of the general formula R-C(O)-XR'. Highest rates of hydrolysis have been obtained with the O-phenyl esters CH(3)(CH(2))(n = 1 - 2)-S-(CH(2))(n = 1 - 2) C(O)-O-phenyl and their thioester analogs in studies where R, X and R' have been varied. The phenol release has been measured with 4-aminoantipyrine and potassium ferricyanide to determine the rates of O-phenyl ester hydrolyses. Thioester hydrolyses have been monitored continuously with 5,5'-dithio-bis (2-nitrobenzoic acid).
Borhan
B
B
Department of Chemistry, University of California, 95616, Davis, CA, USA.
Hammock
B
B
Seifert
J
J
Wilson
B W
BW