[Factors influencing platelet procoagulant activity in type II diabetic patients. The role of sodium-proton exchange].
The aim of our study was the estimation of platelet sodium-proton exchanger activity and platelet pro-coagulant activity, expressed as the availability of platelet factor 3 (PF3) and thrombin generation, in 83 type 2 diabetic patients (mean age 56.7 +/- 7.8 years) and 40 healthy subjects (mean age 54.4 +/- 6.2 years). Thrombin generation was measured in platelet rich plasma, using a chromogenic substrate S-2238. The availability of PF3 was estimated in platelet rich plasma, platelet poor plasma and platelet filtrated plasma, to assess procoagulant activity connected with platelets and cell derived microparticles, shedding upon activation (according to Jy and Horstman). The activity of platelet Na+/H+ exchanger was measured using an optical swelling assay. We found that the activity of PF3 and phospholipid dependent thrombin generation were significantly higher in diabetic patients, irrespective of their vascular complications and metabolic control. The highest increase of PF3 activity was observed in platelet poor (p < 0.0001) and platelet filtrated plasma (p < 0.000001). Na+/H+ exchange rate was significantly higher in diabetic patients in comparison to the controls (4.29 +/- 0.71 x 10(-3)/s vs 3.21 +/- 0.64 x 10(-3)/s, p < 0.00001). There was also a positive correlation between Na+/H+ exchanger activity and PF3 activity in all plasma fractions. Our results suggest that increased thrombin generation, enhanced platelet Na+/H+ exchanger activity and raised PF3 availability, connected mainly with cell derived microparticles, may enhance the risk of vascular damage in type 2 diabetic patients.
Telejko
Beata
B
Klinika Endokrynologii, Diabetologii i Chorób Wewnetrznych AM 15-276 Białystok. telejkob@poczta.onet.pl
Kinalska
Ida
I
Stelmach
Halina
H
Tomasiak
Marian
M
D000328:Adult; D015415:Biomarkers / Q000097:blood; D001777:Blood Coagulation; D001792:Blood Platelets / Q000378:metabolism*; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000503:physiopathology; D006801:Humans; D008875:Middle Aged; D010977:Platelet Factor 3 / Q000378:metabolism*; D011044:Poland; D011522:Protons; D012307:Risk Factors; D012964:Sodium / Q000097:blood; D017923:Sodium-Hydrogen Exchangers / Q000097:blood*; D013917:Thrombin / Q000378:metabolism*
D015415:Biomarkers; D011522:Protons; D017923:Sodium-Hydrogen Exchangers; D010977:Platelet Factor 3; D012964:Sodium; D013917:Thrombin