title: Flexible polyimide probes with microelectrodes and embedded microfluidic channels for simultaneous drug delivery and multi-channel monitoring of bioelectric activity.
abstract: The study of intracellular communication requires devices that can not only monitor the bioelectric activity, but also control and observe the biochemical environment at the cellular level. This paper reports on the development and characterisation of implantable polyimide microprobes that allow simultaneous, selective chemical delivery/probing and multi-channel recording/stimulation of bioelectric activity. The key component of the system is a flexible polyimide substrate with embedded microchannels that is batch-fabricated combining polyimide micromachining and a lamination technique. The devices provide platinum microelectrodes on both sides of the polyimide substrate with an active surface between 20 microm x 20 microm and 50 microm x 50 microm. The embedded microchannels permit highly localised drug delivery or probing at the tip of the device via channel outlets adjacent to the microelectrodes. The microelectrodes were characterised by electrical impedance spectroscopy and the microchannels were studied in microflow experiments. Two different fluid delivery schemes were explored in two different designs. The first device type consists of a simple combination of microchannels and microelectrodes on one substrate. Liquids are ejected at the tip of the device by pressure injection techniques. The second device was inspired by the so-called U-tube concept allowing for highly localised delivery of controlled amounts of liquids in the picoliters range. Thus, the influence of chemical compounds on the electrical activity of cells can be studied with high temporal and spatial resolution. The flexible, implantable devices can be used for studying the chemical and electrical information exchange and communication of cells in in vivo and in vitro experiments.
authors.affiliation: Swiss Federal Institute of Technology-Lausanne, Institut de Microélectronique et de Microsystèmes, Laboratoire de Microsystèmes, EPFL STI-IMM-LMIS4, 1015 Lausanne, Switzerland. stefan.metz@epfl.ch
authors.forename: S
authors.initials: S
authors.lastname: Metz
authors.forename: A
authors.initials: A
authors.lastname: Bertsch
authors.forename: D
authors.initials: D
authors.lastname: Bertrand
authors.forename: Ph
authors.initials: P
authors.lastname: Renaud
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D016503:Drug Delivery Systems / Q000295:instrumentation*; D017097:Electric Impedance; D008839:Microelectrodes; D044085:Microfluidics / Q000295:instrumentation*; D012117:Resins, Synthetic; D013057:Spectrum Analysis; D013997:Time Factors
chemical_list: D012117:Resins, Synthetic; C029945:polyimide resin