Molecular cloning of a beta-glucan pattern-recognition lipoprotein from the white shrimp Penaeus (Litopenaeus) vannamei: correlations between the deduced amino acid sequence and the native protein structure.
The hemolymph pattern-recognition beta-glucan binding protein from the white shrimp Penaeus (Litopenaeus) vannamei is also a high density lipoprotein (betaGBP-HDL) involved in innate immunity. The betaGBP-HDL full length cDNA sequence determined was 6.3 kb long, and contains a long 3'UTR region with a polyadenylation signal and a poly-A+ tail. The open reading frame is 1454 amino acids long and the N-terminal residue of the mature protein is localized in position 198 of the ORF. Comparison of the betaGBP-HDL amino acid sequence against GenBank detected only significant similarity to betaGBP from the crayfish Pacifastacus leniusculus. betaGBP-HDL is expressed in hepatopancreas, muscle, pleopods and gills, but not in hemocytes as determined by RT-PCR. We discuss the analysis of the deduced primary sequence in terms of the predicted secondary structure, glucanase-like and RGD motives relevant to its dual roles in defence and lipid transport.
Romo-Figueroa
María Gabriela
MG
Molecular Biology of Aquatic Organisms, DTAOA, Centro de Investigación en Alimentación y Desarrollo, A.C., P.O. Box 1735, Hermosillo, Sonora 83000, México.
Vargas-Requena
Claudia
C
Sotelo-Mundo
Rogerio R
RR
Vargas-Albores
Francisco
F
Higuera-Ciapara
Inocencio
I
Söderhäll
Kenneth
K
Yepiz-Plascencia
Gloria
G
D000595:Amino Acid Sequence; D000818:Animals; D003400:Astacoidea / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D005936:Glucans / Q000235:genetics / Q000378:metabolism; D006458:Hemolymph / Q000276:immunology*; D037102:Lectins; D008075:Lipoproteins, HDL / Q000235:genetics*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000235:genetics; D033561:Penaeidae / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D002352:Carrier Proteins; D005936:Glucans; D037102:Lectins; D008075:Lipoproteins, HDL; D009842:Oligopeptides; C037937:glucan-binding proteins; C047981:arginyl-glycyl-aspartic acid