Imaging wound healing using optical coherence tomography and multiphoton microscopy in an in vitro skin-equivalent tissue model.
Laser thermal injury and subsequent wound healing in organotypic, skin-equivalent tissue models were monitored using optical coherence tomography (OCT), multiphoton microscopy (MPM), and histopathology. The in vitro skin-equivalent raft tissue model was composed of dermis with type I collagen and fibroblast cells and epidermis of differentiated keratinocytes. Noninvasive optical imaging techniques were used for time-dependent, serial measurements of matrix destruction and reconstruction and compared with histopathology. The region of laser thermal injury was clearly delineated in OCT images by low signal intensity. High resolution MPM imaging using second-harmonic generation revealed alterations in collagen microstructure organization with subsequent matrix reconstruction. Fibroblast cell migration in response to injury was monitored by MPM using two-photon excited fluorescence. This study illustrates the complementary features of linear and nonlinear light-tissue interaction in intrinsic signal optical imaging and their use for noninvasive, serial monitoring of wound healing processes in biological tissues.
Yeh
Alvin T
AT
University of California, Beckman Laser Institute, Laser Microbeam and Medical Program, 1002 Health Sciences Road East, Irvine, California 92612, USA. ayeh@laser.bli.uci.edu
Kao
Bunsho
B
Jung
Woong Gyu
WG
Chen
Zhongping
Z
Nelson
J Stuart
JS
Tromberg
Bruce J
BJ
D002056:Burns / Q000209:etiology / Q000473:pathology* / Q000503:physiopathology*; D002478:Cells, Cultured; D006801:Humans; D007834:Lasers; D008853:Microscopy / Q000379:methods*; D017785:Photons; D012867:Skin / Q000473:pathology* / Q000503:physiopathology*; D041623:Tomography, Optical Coherence; D014945:Wound Healing