title: The inner nuclear membrane protein lamin B receptor forms distinct microdomains and links epigenetically marked chromatin to the nuclear envelope.
abstract: Using heterochromatin-enriched fractions, we have detected specific binding of mononucleosomes to the N-terminal domain of the inner nuclear membrane protein lamin B receptor. Mass spectrometric analysis reveals that LBR-associated particles contain complex patterns of methylated/acetylated histones and are devoid of "euchromatic" epigenetic marks. LBR binds heterochromatin as a higher oligomer and forms distinct nuclear envelope microdomains in vivo. The organization of these membrane assemblies is affected significantly in heterozygous ic (ichthyosis) mutants, resulting in a variety of structural abnormalities and nuclear defects.
authors.affiliation: Laboratory of Biology, The University of Ioannina, School of Medicine, 45 110 Ioannina, Greece.
authors.forename: Dimitra
authors.initials: D
authors.lastname: Makatsori
authors.forename: Niki
authors.initials: N
authors.lastname: Kourmouli
authors.forename: Hara
authors.initials: H
authors.lastname: Polioudaki
authors.forename: Leonard D
authors.initials: LD
authors.lastname: Shultz
authors.forename: Kelvin
authors.initials: K
authors.lastname: McLean
authors.forename: Panayiotis A
authors.initials: PA
authors.lastname: Theodoropoulos
authors.forename: Prim B
authors.initials: PB
authors.lastname: Singh
authors.forename: Spyros D
authors.initials: SD
authors.lastname: Georgatos
mesh_terms: D000818:Animals; D006367:HeLa Cells; D006570:Heterochromatin / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D009685:Nuclear Envelope / Q000737:chemistry*; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry*; D000097682:Lamin B Receptor
chemical_list: D006570:Heterochromatin; D018160:Receptors, Cytoplasmic and Nuclear; D000097682:Lamin B Receptor