NO-dependent phototoxicity of Roussin's black salt against cancer cells.
A metal-nitrosyl complex, Roussin's black salt (RBS), releases nitric oxide after illumination. Approximately 3.7 NO molecules were released from one RBS molecule. Both short- and long-term effects of photogenerated NO on the two neoplastic cell lines: human (SK-MEL188) and mouse (S91) have been investigated. Exogenous NO from RBS was toxic to cells in a dose-dependent manner. Apoptotic damage predominates in the response to the injury, as shown by TUNEL assay. NO and its short-lived metabolites, but not other RBS photoproducts, are responsible for cellular death. RBS in dark was toxic to cells at concentrations above 1 microM. This relatively high cytotoxicity of RBS in the dark prevents its application as a systemic anticancer agent in vivo, unless it is applied locally.
Janczyk
Agnieszka
A
Faculty of Chemistry, Jagiellonian University, Krakow, Poland.
Wolnicka-Glubisz
Agnieszka
A
Chmura
Antonina
A
Elas
Martyna
M
Matuszak
Zenon
Z
Stochel
Grazyna
G
Urbanska
Krystyna
K
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D017484:Dermatitis, Phototoxic / Q000209:etiology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D058085:Iron Compounds / Q000494:pharmacology* / Q000528:radiation effects / Q000627:therapeutic use; D008545:Melanoma / Q000188:drug therapy / Q000473:pathology*; D051379:Mice; D009369:Neoplasms / Q000188:drug therapy / Q000473:pathology; D009569:Nitric Oxide / Q000494:pharmacology*; D020030:Nitric Oxide Donors / Q000494:pharmacology / Q000528:radiation effects / Q000627:therapeutic use; D009603:Nitroso Compounds / Q000494:pharmacology* / Q000528:radiation effects / Q000627:therapeutic use
D058085:Iron Compounds; D020030:Nitric Oxide Donors; D009603:Nitroso Compounds; D009569:Nitric Oxide; C078952:Roussin's Black Salt