title: Gene- and tissue-specific alterations of circadian clock gene expression in streptozotocin-induced diabetic mice under restricted feeding.
abstract: Circadian clocks are located in the suprachiasmatic nucleus (SCN) of the hypothalamus as well as in the peripheral tissues of mammals. Recent molecular studies have revealed that the phase of circadian gene expressions in peripheral clocks could entrain to time-imposed restricted feeding (RF) independently of the SCN. To elucidate whether endogenous insulin is involved in the entraining mechanisms of peripheral clocks to RF, we examined the expression profiles of clock genes in peripheral tissues of mice with diabetes induced by streptozotocin (STZ). The circadian expressing genes (mPer1, mPer2, and BMAL1) underwent a phase-shift induced by RF in the heart, liver, and kidney of both diabetic and normal animals. However, the expression phase of mPer1 in these tissues of the diabetic mice significantly differed from those in the normal animals under RF. The expression phase of the circadian output gene, albumin D-site binding protein (DBP), was completely shifted by RF both in normal and in diabetic mice, suggesting that the endogenous insulin is not essential for the entrainment of peripheral clocks to feeding cycles in mice.
authors.affiliation: Clock Cell Biology Research Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Katsutaka
authors.initials: K
authors.lastname: Oishi
authors.forename: Manami
authors.initials: M
authors.lastname: Kasamatsu
authors.forename: Norio
authors.initials: N
authors.lastname: Ishida
mesh_terms: D056930:ARNTL Transcription Factors; D000222:Adaptation, Physiological; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D001683:Biological Clocks; D001786:Blood Glucose / Q000032:analysis*; D018797:Cell Cycle Proteins; D002940:Circadian Rhythm; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D004326:Drinking; D004435:Eating; D002149:Energy Intake; D005508:Food Deprivation; D005786:Gene Expression Regulation; D007328:Insulin / Q000097:blood*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009206:Myocardium / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D009928:Organ Specificity; D056950:Period Circadian Proteins; D013311:Streptozocin; D014018:Tissue Distribution; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D056930:ARNTL Transcription Factors; D051792:Basic Helix-Loop-Helix Proteins; D001786:Blood Glucose; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D007328:Insulin; D009687:Nuclear Proteins; D056950:Period Circadian Proteins; D013311:Streptozocin; D014157:Transcription Factors; C538961:Bmal1 protein, mouse; C498260:Dbp protein, mouse; C539023:Per1 protein, mouse; C513312:Per2 protein, mouse