title: The effect of temperature, pressure, exposure time, and depressurization rate on lipase activity in SCCO2.
abstract: We investigated the influence of temperature, pressure, exposure time, and decompression rate on lipase activity in high-pressure CO2 medium. A high-pressure, variable-volume view cell was employed in the experiments, varying the temperature from 30 to 70 degrees C in the pressure range of 70-250 bar at various high-pressure exposure times (60-360 min) and adopting several decompression rates (10-200 kg/[m3 x min]). The results obtained show that an increase in temperature and density led to an enhancement of enzyme activity losses while the decompression rates had a weak influence on enzyme inactivation.
authors.affiliation: Department of Food Engineering, URI-Campus de Erechim, Av. Sete de Setembro, 1621-Erechim-RS, 99700-000, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Lanza
authors.forename: Wagner Luís
authors.initials: WL
authors.lastname: Priamo
authors.forename: José
authors.initials: J
authors.lastname: Vladimir Oliveira
authors.forename: Cláudio
authors.initials: C
authors.lastname: Dariva
authors.forename: Débora
authors.initials: D
authors.lastname: de Oliveira
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002175:Candida / Q000201:enzymology; D002245:Carbon Dioxide / Q000737:chemistry*; D016014:Linear Models; D008049:Lipase / Q000737:chemistry*; D011312:Pressure; D013696:Temperature; D013997:Time Factors
chemical_list: D002245:Carbon Dioxide; D008049:Lipase