title: Morphological analysis of spine shapes of Purkinje cell dendrites in the rat cerebellum using high-voltage electron microscopy.
abstract: Morphological changes in spine shapes have been implicated as indications for physiological or pathological status. To investigate the normal distribution ratio of spine shapes of rat Purkinje cells, morphological analysis was conducted using high-voltage electron microscopy following Golgi impregnation. Spines were classified into thin, stubby, mushroom, branched, and unclassified type by their distinct morphological features. In the tertiary branches of Purkinje cell dendrites, proportions of each category were 69.11+/-1.38% (thin), 13.5+/-1.23% (stubby), 10.45+/-0.74% (mushroom), 2.21+/-0.31% (branched), and 4.73+/-0.52% (unclassified). These results suggest that dendritic spines of Purkinje cells may tend to cluster in defined groups by shapes implying that different spine shapes could reflect different functional roles. This classification could be applied for further study of spine plasticity in various conditions.
authors.affiliation: Institute of Human Genetics and Department of Anatomy, Division of Brain Korea 21 Biomedical Science, Korea University College of Medicine, 126-1 Anam-Dong 5-Ga, Seongbuk-Ku, Seoul, 136-705, South Korea.
authors.forename: Kea Joo
authors.initials: KJ
authors.lastname: Lee
authors.forename: Hyun
authors.initials: H
authors.lastname: Kim
authors.forename: Tae Sik
authors.initials: TS
authors.lastname: Kim
authors.forename: Sun-Hwa
authors.initials: SH
authors.lastname: Park
authors.forename: Im Joo
authors.initials: IJ
authors.lastname: Rhyu
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000648:ultrastructure; D003712:Dendrites / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron / Q000379:methods*; D011689:Purkinje Cells / Q000648:ultrastructure*; D051381:Rats; D017207:Rats, Sprague-Dawley