Liquid chromatography/photodiode array detection for determination of strychnine in blood: a fatal case report.
An original liquid chromatography method with photodiode-array detection (DAD) is presented for the determination of strychnine in blood. This sensitive method allows the use of only 0.1 ml of sample. The strychnine was isolated from blood using a liquid-liquid extraction procedure and chloroquine as an internal standard. The limits of detection (LOD) and quantification were 0.06 and 0.5 mg/l, respectively. The recovery was 94% and the coefficients of variation (CV) ranged from 5.9 to 10.8%. A fatal case of strychnine poisoning is presented, with a lethal blood concentration of 25 mg/l.
Duverneuil
Charlotte
C
Laboratoire de Pharmacologie-Toxicologie, Centre Hospitalier Universitaire Raymond Poincaré, 104 Boulevard R. Poincaré, 92380 Garches, France.
de la Grandmaison
Geoffroy Lorin
GL
de Mazancourt
Philippe
P
Alvarez
Jean-Claude
JC
D000328:Adult; D000962:Antimalarials; D002738:Chloroquine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005554:Forensic Medicine / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D011041:Poisoning / Q000175:diagnosis; D011042:Poisons / Q000097:blood*; D013331:Strychnine / Q000097:blood* / Q000506:poisoning*
D000962:Antimalarials; D007202:Indicators and Reagents; D011042:Poisons; D002738:Chloroquine; D013331:Strychnine