title: The design of a novel complementary metal oxide semiconductor detection system for biochemical luminescence.
abstract: We designed a complementary metal oxide semiconductor (CMOS) chip with accompanied accessories as a system for the detections and quantifications of biochemical luminescence. This is the first of such instruments that has been reported. The semiconductor chip was manufactured through a 0.25 microm CMOS standard process. A current mirror was designed in integrated circuit (IC) to amplify the signal current that was induced by chemiluminescence. Horseradish peroxidase (HRP)-luminol-H2O2 system was used as an example to constitute a useful platform for coupling to chemiluminescence reactions which produce H2O2. Glucose-glucose oxidase (GOD) reaction was coupled with HRP-luminol-H2O2 reaction to demonstrate the ability of the novel CMOS base instrument for quantifying the biological luminescence of a variety of valuable clinical assays. Our results illustrated that the combination of the specifically designed CMOS IC and commercially available electronic devices established a simple and useful bioanalytical tool.
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Institute of Biochemical Engineering, 75 Po-Ai Street, Hsinchu, Taiwan.
authors.forename: Ude
authors.initials: U
authors.lastname: Lu
authors.forename: Ben C-P
authors.initials: BC
authors.lastname: Hu
authors.forename: Yu-Chuan
authors.initials: YC
authors.lastname: Shih
authors.forename: Chung-Yu
authors.initials: CY
authors.lastname: Wu
authors.forename: Yuh-Shyong
authors.initials: YS
authors.lastname: Yang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D005947:Glucose / Q000032:analysis; D006861:Hydrogen Peroxide / Q000032:analysis; D007700:Kinetics; D008163:Luminescent Measurements; D010087:Oxides; D012666:Semiconductors; D013997:Time Factors
chemical_list: D010087:Oxides; D006861:Hydrogen Peroxide; D005947:Glucose