title: Recent advances in the biocatalytic reduction of ketones and oxidation of sec-alcohols.
abstract: To improve the efficiency and applicability of biocatalytic redox-reactions for asymmetric ketone-reduction and enantioselective alcohol-oxidation catalyzed by nicotinamide-dependent dehydrogenases/reductases, several achievements for cofactor-recycling have been made during the last two years. First, the use of hydrogenases for NADPH recycling in a two enzyme system. Second, preparative transformations with alcohol dehydrogenases coupled with NADH oxidases for NAD+/NADP+ recycling. Third, an exceptional chemo-stable alcohol dehydrogenase can efficiently use i-propanol and acetone as cosubstrates for reduction and oxidation, respectively, in a single-enzyme system. Novel carbonyl reductases and dehydrogenases derived from plant cells are particularly suited for sterically demanding substrates.
authors.affiliation: Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Austria.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Kroutil
authors.forename: Harald
authors.initials: H
authors.lastname: Mang
authors.forename: Klaus
authors.initials: K
authors.lastname: Edegger
authors.forename: Kurt
authors.initials: K
authors.lastname: Faber
mesh_terms: D000438:Alcohols / Q000737:chemistry / Q000378:metabolism*; D001419:Bacteria / Q000201:enzymology; D001711:Biotransformation; D002384:Catalysis; D007659:Ketones / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism
chemical_list: D000438:Alcohols; D007659:Ketones; D010088:Oxidoreductases