title: Upregulation of rhoA mRNA in bronchial smooth muscle of antigen-induced airway hyperresponsive rats.
abstract: It has recently been suggested that RhoA plays an important role in the enhancement of Ca2+ sensitization observed in smooth muscle contraction. In the present study, the expression of rhoA mRNA in the bronchial smooth muscle of antigen-induced airway hyperresponsive rats was compared with that of control animals. Reverse transcription-polymerase chain reaction experiments using total RNA from these tissue specimens and the specific primers revealed rhoA mRNA to be expressed in bronchial smooth muscle of the rat. The rhoA mRNA expression in bronchial smooth muscle of the hyperresponsive rats was significantly increased in comparison to that of control animals. It is thus possible that upregulation of RhoA protein might be involved in the mechanism underlying the increased contractility of the bronchial smooth muscle which occurs with airway hyperresponsiveness.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan. chiba@hoshi.ac.jp
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Chiba
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Sakai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Wachi
authors.forename: Hideki
authors.initials: H
authors.lastname: Sugitani
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Seyama
authors.forename: Miwa
authors.initials: M
authors.lastname: Misawa
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D001980:Bronchi / Q000378:metabolism*; D016535:Bronchial Hyperreactivity / Q000276:immunology* / Q000378:metabolism*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D008297:Male; D009130:Muscle, Smooth / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation; D020742:rhoA GTP-Binding Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D000941:Antigens; D012333:RNA, Messenger; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D020742:rhoA GTP-Binding Protein