[Expression of survivin in human gastric adenocarcinomas: correlation with proliferation and apoptosis].
OBJECTIVE
To investigate the expression and significance of survivin, ki-67 and apoptosis index in patients with advanced gastric adenocarcinoma.


METHODS
Immunohistochemical SP method for survivin expression as well as cell proliferative index (ki-67) and apoptosis index (TUNEL) was conducted on 120 gastric adenocarcinomas.


RESULTS
The survivin was detected in the cytoplasm of carcinoma cells in 59 (49.17%) of the 120 gastric adenocarcinomas, in 32 (64.00%) of the lymph node metastasis, and in 21 (17.50%) of the 120 basal layer in normal gastric mucosa, respectively. The mean proliferative index (ki-67) in primary tumors was 7.55%, which was significantly lower than the mean proliferative index of 8.34% observed in lymph node metastasis. The mean apoptosis index in primary tumors was 1.16%, which was significantly higher than the mean apoptosis index of 0.89% observed in lymph node metastasis. The frequency of survivin expression was significantly higher in lymph node metastasis than in primary gastric adenocarcinoma. Expression of survivin was significantly correlated with histological subtypes, the depth of invasion, or lymph node metastasis (P < 0.05). There was negative correlation between weighted survivin score and apoptosis index (P < 0.05), but no correlation with proliferative index.


CONCLUSIONS
The high level expression of survivin might be a referenced indicator in evaluating differentiation of tumor and in predicting lymph nodes metastasis and estimating apoptosis index.
Yao
Xue-quan
XQ
Institute of General Surgery, Nanjing General Hospital of People's Liberation Army, Medical School of Nanjing University, Nanjing 210002, China.
Liu
Fu-kun
FK
Qi
Xiao-ping
XP
Wu
Bo
B
Yin
Hong-lin
HL
Ma
Heng-hui
HH
Shi
Qun-li
QL
Zhou
Xiao-jun
XJ
Li
Jie-shou
JS
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D000368:Aged; D017209:Apoptosis; D005260:Female; D005753:Gastric Mucosa / Q000737:chemistry / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D051034:Inhibitor of Apoptosis Proteins; D019394:Ki-67 Antigen / Q000032:analysis; D008198:Lymph Nodes / Q000737:chemistry / Q000473:pathology; D008297:Male; D008869:Microtubule-Associated Proteins / Q000032:analysis*; D008875:Middle Aged; D009363:Neoplasm Proteins; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D000077022:Survivin
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D019394:Ki-67 Antigen; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D000077022:Survivin