title: Detection of cytotoxic necrotizing factor types 1 and 2 among fecal Escherichia coli isolates from Brazilian children with and without diarrhea.
abstract: The enteropathogenic role of cytotoxic necrotizing factor (CNF)-producing Escherichia coli was investigated by searching cnf genes among 2074 isolates from 200 children with and 200 without acute diarrhea in Brazil. Fourteen (7%) cases versus 10 (5%) control children carried at least one cnf positive isolate (P = 0.50) and most isolates expressed CNF type 1. DNA sequences of virulence factors of extraintestinal pathogenic E. coli (ExPEC) were detected in 78.6% of CNF1-producing isolates. Besides not being associated with human acute diarrhea, the CNF1-producing isolates here identified may represent potential ExPEC transitorily composing the normal intestinal flora.
authors.affiliation: Seção de Bacteriologia, Instituto Adolfo Lutz, São Paulo, SP, 01246-902, Brasil.
authors.forename: Ana Terezinha
authors.initials: AT
authors.lastname: Tavechio
authors.forename: Lilian Regina M
authors.initials: LR
authors.lastname: Marques
authors.forename: Cecília Mari
authors.initials: CM
authors.lastname: Abe
authors.forename: Tânia A T
authors.initials: TA
authors.lastname: Gomes
mesh_terms: D000208:Acute Disease; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D003603:Cytotoxins / Q000096:biosynthesis* / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D003967:Diarrhea / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins; D005243:Feces / Q000382:microbiology; D006801:Humans; D007223:Infant
chemical_list: D001427:Bacterial Toxins; D003603:Cytotoxins; D015342:DNA Probes; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C086995:cytotoxic necrotizing factor type 2; C060296:cytotoxic necrotizing factor type 1