[Colorimetric micromethod for serum iodine determination].
The measurement of iodine is a widely accepted method to explore iodine disorders. The most precise estimation is the determination of the urinary iodine in 24-hour collections. Urine collection is notoriously difficult to obtain, specially in children. In these conditions, serum measurement could be a method to overcome these limitations. We describe a colorimetric method adapted on a microtiter plate, with optimized serum mineralization conditions. The method is linear to 2400 nmol/L with a detection limit of 75 nmol/L. Precision is below 10%. The method was validated against one automatic technique. We conclude that this relatively simple method could be an additional tool to explore dysthyroidism.
Houzé
P
P
Laboratoire de biochimie A, Hôpital Saint-Louis, 1, avenue Claude Vellefaux, 75010 Paris. pascal.houze@sls.ap-hop-paris.fr
Corvisier
M
M
Toubert
M-E
ME
Gourmel
B
B
Bousquet
B
B
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001774:Blood Chemical Analysis / Q000379:methods; D003124:Colorimetry / Q000379:methods*; D006801:Humans; D007455:Iodine / Q000097:blood*; D008875:Middle Aged
D007455:Iodine