Seeing 'cool' and 'hot'--infrared thermography as a tool for non-invasive, high-throughput screening of Arabidopsis guard cell signalling mutants.
The use of Arabidopsis mutants defective in abscisic acid (ABA) perception has been instrumental in the understanding of stomatal function, in particular, ABA signalling in guard cells. The considerable attention devoted to ABA signalling in guard cells is due in part to (1) the fundamental role of ABA in drought stress and (2) the use of a screening protocol based on the sensitivity of seed germination to ABA. Such a screen has facilitated the isolation of ABA signalling mutants with genetic lesions that exert pleiotropic effects at the whole plant level. As such, there is a requirement for new approaches to complement the seed germination screen. The recent advances made in the use of infrared thermography as a non-invasive, high-throughput tool are reviewed here and the versatility of this technique for screening Arabidopsis defective in stomatal regulation is highlighted.
Wang
Yibing
Y
Department of Biological Sciences, Institute of Environmental and Natural Sciences, Lancaster University, Bailrigg, Lancaster LA1 4YQ, UK.
Holroyd
Geoffrey
G
Hetherington
Alistair M
AM
Ng
Carl K-Y
CK
D000040:Abscisic Acid / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics*; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant; D006358:Hot Temperature; D006813:Humidity; D007259:Infrared Rays; D008954:Models, Biological; D010641:Phenotype; D018515:Plant Leaves / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013817:Thermography
D000040:Abscisic Acid