title: Effect of N-acetylglucosaminyltransferase V on the expressions of other glycosyltransferases.
abstract: Transfection of sense cDNA of N-acetylglucosaminyltransferase V (GnTV) into H7721 human hepatocellular carcinoma cells resulted in the decreased expression of surface sialyl Lewis X (SLe(x)), a sialylated fucose-containing antigen. The enzymatic mechanisms were speculated to be the concomitantly decreased expression of alpha1,3-fucosyltransferase (FucT)-III, -VI, -VII and the branching enzyme of O-glycans, core 2-beta1,6-N-acetylglucosaminyltransferase (C2GnT)-I, -II. These two glycosyltransferase families were suggested to be the key enzymes in the synthesis of SLe(x). The expression of alpha2,3-sialyltransferase (ST3)-IV, but not ST3-I, -II and -III was elevated by sense GnTV. However, it did not cause the increase of SLe(x) synthesis. Transfection of antisense GnTV into H7721 cells showed entirely opposite effects on the expression of above-mentioned SLe(x) and glycosyltransferases as the sense GnTV.
authors.affiliation: Key Laboratory of Glycoconjugate Research, Ministry of Health, Department of Biochemistry, Shanghai Medical College, Fudan University, Shanghai 200032, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Guo
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zong-hou
authors.initials: ZH
authors.lastname: Shen
authors.forename: Xia-ying
authors.initials: XY
authors.lastname: Zhang
authors.forename: Hui-li
authors.initials: HL
authors.lastname: Chen
mesh_terms: D002236:Carbohydrate Conformation; D045744:Cell Line, Tumor; D016373:DNA, Antisense / Q000378:metabolism; D016695:Glycosyltransferases / Q000378:metabolism*; D006801:Humans; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism*; D000080506:Sialyl Lewis X Antigen; D014162:Transfection
chemical_list: D016373:DNA, Antisense; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; D016695:Glycosyltransferases; D017351:N-Acetylglucosaminyltransferases; C052254:alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase