title: Application of capillary electrophoresis to the determination of various benzylpenicillin salts.
abstract: The application of capillary electrophoresis for separation of benzylpenicillin, procaine, benzathine and clemizole was investigated. Phosphate-borate buffer supplemented with sodium dodecyl sulphate 14.4 g/l and electrophoresis voltage 18 kV seem to provide optimal conditions for micellar electrokinetic chromatographic assay of penicillin salts. This method is selective and precise. The results obtained from CE method recovery assay (above 98% for all but procaine-97% substances) and from determination of benzylpenicillin by CE compared with HPLC results, confirmed good accuracy.
authors.affiliation: Department of Antibiotics and Microbiology, National Institute of Public Health, 30/34 Chełmska St. 00-725 Warsaw, Poland. tyski@il.waw.pl
authors.forename: Genowefa
authors.initials: G
authors.lastname: Pajchel
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Michalska
authors.forename: Stefan
authors.initials: S
authors.lastname: Tyski
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D010400:Penicillin G / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D010400:Penicillin G