Reproducible prediction of contact allergenic potency using the local lymph node assay.
A considerable body of data has been accumulated which demonstrates that the local lymph node assay (LLNA) can provide a valuable estimation of the contact allergenic potency of a substance. This estimate is obtained via interpolation of the LLNA dose-response curve and is expressed as the concentration of the chemical required to evince a 3-fold stimulation of proliferation in lymph nodes draining the site of application compared to the vehicle-treated controls (EC3). It has also been shown that the EC3 estimates are reproducible and are stable over time. In the present work, we have extended this information by a demonstration of the inherent biological variability surrounding EC3 estimation, using data derived (from a single laboratory) from the testing of isoeugenol as a positive control. Isoeugenol gave EC3 values ranging from 0.5 to 2.6% (n = 29), with a mean and standard deviation of 1.2 +/- 0.6%. Given that EC3 values for a variety of contact allergens range over several orders of magnitude, these results further endorse the utility of EC3 values as a reliable indicator of human contact allergenic potency.
Basketter
David A
DA
Safety and Environmental Assurance Centre, Unilever Colworth, Sharnbrook, Bedford, UK. david.basketter@unilever.com
Cadby
Peter
P
D000287:Administration, Topical; D000485:Allergens / Q000008:administration & dosage / Q000009:adverse effects*; D000818:Animals; D017449:Dermatitis, Allergic Contact / Q000175:diagnosis* / Q000209:etiology; D005054:Eugenol / Q000008:administration & dosage / Q000009:adverse effects* / Q000031:analogs & derivatives*; D005260:Female; D023441:Local Lymph Node Assay; D051379:Mice; D008808:Mice, Inbred CBA; D011237:Predictive Value of Tests; D015203:Reproducibility of Results
D000485:Allergens; D005054:Eugenol; C036643:isoeugenol