Molecular characterization of a pathogenesis-related protein 8 gene encoding a class III chitinase in rice.
A cDNA encoding a class III chitinase (Oschib1) was isolated from a cDNA library constructed from rice leaves infected with the blast fungus Magnaporthe grisea. The cDNA contains an open reading frame of 861 nucleotides encoding 286 amino acid residues with a pI of 5.06. The deduced amino acid sequence of Oschibl has a high level of similarity with class IIIb chitinases of Gladiolus gandavensis (46%) and Tulipa bakeri (49%). A high level of Oschibl mRNA was detected after inoculation with M. grisea or Xanthomonas oryzae pv. oryzae. Expression of Oschib1 was induced more rapidly when an avirulent strain of M. grisea was inoculated (incompatible interaction) than when a virulent strain was used (compatible interaction). Expression of Oschibl was also induced by treatment of signaling molecules such as salicylic acid, ethylene, and methyl jasmonic acid, and by treatment with H2O2 or CuSO4. The induction patterns of Oschibl expression suggest that Oschib1 may be involved in defense response against pathogen infections and may be classified as a member of pathogenesis-related protein 8 in rice.
Park
Chan-Ho
CH
School of Agricultural Biotechnology and Center for Agricultural Bio-Materials, Seoul National University, Seoul 151-742, Korea.
Kim
Soonok
S
Park
Ju-Young
JY
Ahn
Il-Pyung
IP
Jwa
Nam-Soo
NS
Im
Kyung-Hwan
KH
Lee
Yong-Hwan
YH
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002688:Chitinases / Q000737:chemistry / Q000502:physiology*; D004247:DNA / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D015723:Gene Library; D006861:Hydrogen Peroxide / Q000494:pharmacology; D020082:Magnaporthe / Q000378:metabolism; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology; D010802:Phylogeny; D010940:Plant Proteins; D012313:RNA / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D015398:Signal Transduction; D013997:Time Factors; D015854:Up-Regulation
D018076:DNA, Complementary; D010940:Plant Proteins; D012313:RNA; D004247:DNA; D006861:Hydrogen Peroxide; D002688:Chitinases; C110892:chitinase, class III