title: Rat endometrial Vdup1 expression: changes related to sensitization for the decidual cell reaction and hormonal control.
abstract: During implantation in rodents, attachment and invasion of embryonic trophoblast is accompanied by decidualization of the adjacent endometrial stroma. Decidualization can be initiated only when the endometrium is receptive, and this occurs for a short period in pregnancy. The molecular mechanisms underlying this phenomenon remain unclear. In the current study, using differential display and northern blot analysis, we found that steady-state levels of mRNA for vitamin D3 upregulated protein 1 (Vdup1) were significantly higher in 'refractory' and 'delayed' endometrium compared with 'receptive' endometrium or endometrium undergoing artificially induced decidualization. Conversely, thioredoxin (Txn), a ubiquitously expressed cellular redox regulator known to promote growth and proliferation, was found to have elevated transcript levels within the decidualizing endometrium. VDUP1 has previously been shown to bind TXN and inhibit its action. In an inverse, but cooperative, relationship, these molecules have been implicated in regulating cell growth and proliferation in a number of tissues and during transformation to cancer. The Vdup1 mRNA is localized to the uterine stroma in the nonreceptive endometrium, the site of increased Txn mRNA levels during decidualization. In addition, Vdup1 mRNA levels are inversely regulated by progesterone and estrogen; prolonged progesterone exposure stimulates an increase in Vdup1 mRNA levels whereas estrogen decreases Vdup1 transcript levels. Together, these results suggest a novel mechanism by which suppression of the decidual response in the nonreceptive endometrium may occur.
authors.affiliation: Physiology and Pharmacology, and Obstetrics and Gynaecology, University of Western Ontario, London, Ontario, Canada, N6A 5C1.
authors.forename: David G
authors.initials: DG
authors.lastname: Simmons
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Kennedy
mesh_terms: D000818:Animals; D015152:Blotting, Northern / Q000379:methods; D002352:Carrier Proteins / Q000235:genetics*; D010064:Embryo Implantation; D004717:Endometrium / Q000737:chemistry*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D015870:Gene Expression; D017403:In Situ Hybridization / Q000379:methods; D010052:Ovariectomy; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013879:Thioredoxins
chemical_list: D002352:Carrier Proteins; D012333:RNA, Messenger; C486715:TXNIP protein, human; D011374:Progesterone; D004958:Estradiol; D013879:Thioredoxins