title: [Expression of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 in the pig coronary artery injury model: comparison of plain old balloon angioplasty and stent implantation].
abstract: OBJECTIVE
  Restenosis after coronary intervention usually occurs due to coronary remodeling or neointimal formation, but inflammation is also important especially after stent implantation. Adhesion molecules are important in the recruitment of inflammatory cells into the neointima and in the phenotypical changes of vascular smooth muscle cells. To examine the role of adhesion molecules in the pathogenesis of restenosis, immunohistochemical expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) was investigated in the pig coronary injury model.
  
  
  METHODS
  Left anterior descending coronary arteries of pigs were injured using a balloon. Two weeks after the injury, balloon injury was performed again in the balloon group and a Palmaz-Schatz stent was implanted in the stent group. Pigs were sacrificed at 1, 2 and 4 weeks. Immunohistochemical analysis was performed using ICAM-1, VCAM-1, macrophage and alpha-smooth muscle actin antibodies.
  
  
  RESULTS
  In non-injured vessels, weak immunoreactivities of ICAM-1 and VCAM-1 were observed in the endothelium and media. In injured sites, ICAM-1 and VCAM-1 were found in the inflammatory cells and smooth muscle cells in the neointima from 1 week, and strong immunoreactivities were seen around the strut in the stent group. Although the immunoreactivities peaked at 2 weeks in the balloon group, strong immunoreactivities were still seen at 4 weeks in the stent group. Regenerated endothelial cells were positive for both antibodies from 2 weeks.
  
  
  CONCLUSIONS
  The expression of ICAM-1 and VCAM-1 lasted longer in the stent group than in the balloon group, suggesting the occurrence of late restenosis after stent implantation. Control of the inflammatory response including adhesion molecules is essential for further reduction of restenosis after stent implantation.
authors.affiliation: Department of Internal Medicine, Division of Cardiology, Showa University, Fujigaoka Hospital, Yokohama.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Shimizu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suzuki
authors.forename: Kohei
authors.initials: K
authors.lastname: Wakabayashi
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Iso
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shibata
authors.forename: Minoru
authors.initials: M
authors.lastname: Yorozuya
authors.forename: Takashi
authors.initials: T
authors.lastname: Katagiri
authors.forename: Youichi
authors.initials: Y
authors.lastname: Takeyama
mesh_terms: D015906:Angioplasty, Balloon, Coronary / Q000009:adverse effects*; D000818:Animals; D023903:Coronary Restenosis / Q000209:etiology*; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D007249:Inflammation / Q000209:etiology / Q000473:pathology; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis* / Q000502:physiology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D015607:Stents / Q000009:adverse effects*; D013552:Swine; D017539:Tunica Intima / Q000378:metabolism / Q000473:pathology; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis / Q000502:physiology*
chemical_list: D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1