title: Real-time PCR analysis of carbon catabolite repression of cellobiose dehydrogenase gene transcription in Trametes versicolor.
abstract: Cellobiose dehydrogenase production in Trametes versicolor is repressed when additional carbon sources, such as glucose, maltose, galactose, arabinose, and xylose, are added to the fungal cultures growing on cellulose. Real-time quantitative reverse transcription-polymerase chain reaction has been used to demonstrate that the addition of galactose, arabinose, and xylose results in 19-, 92-, and 114-fold reductions, respectively, in cdh transcript levels 96 h post-addition. Glucose exhibits the greatest repressive effect, resulting in a 3400-fold decrease in cdh transcript levels.
authors.affiliation: Microbiology Department, University College Cork, National University of Ireland, Cork, Ireland, UK.
authors.forename: P C
authors.initials: PC
authors.lastname: Stapleton
authors.forename: J
authors.initials: J
authors.lastname: O'Mahony
authors.forename: A D W
authors.initials: AD
authors.lastname: Dobson
mesh_terms: D001089:Arabinose / Q000378:metabolism; D002237:Carbohydrate Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D050260:Carbohydrate Metabolism; D004794:Enzyme Repression / Q000235:genetics / Q000502:physiology; D005690:Galactose / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000235:genetics* / Q000502:physiology; D005947:Glucose / Q000378:metabolism; D008320:Maltose / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D020072:Polyporales / Q000201:enzymology* / Q000235:genetics*; D012331:RNA, Fungal / Q000032:analysis / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis / Q000302:isolation & purification; D014158:Transcription, Genetic; D014994:Xylose / Q000378:metabolism
chemical_list: D012331:RNA, Fungal; D012333:RNA, Messenger; D008320:Maltose; D014994:Xylose; D001089:Arabinose; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase; D005947:Glucose; D005690:Galactose