title: Sepsis severity is the major determinant of circulating thrombopoietin levels in septic patients.
abstract: OBJECTIVE
  To measure serum thrombopoietin levels and to investigate their relationship with platelet counts and other potential determinants in septic patients.
  
  
  METHODS
  Prospective study comparing septic patients and healthy volunteers.
  
  
  METHODS
  General intensive care units in two tertiary university hospitals.
  
  
  METHODS
  A total of 152 consecutive septic patients (69 with sepsis, 24 with severe sepsis, and 59 with septic shock). Twenty-two healthy volunteers served as control subjects. Sepsis severity was determined by grading septic patients in those having sepsis, severe sepsis, and septic shock.
  
  
  METHODS
  None.
  
  
  RESULTS
  After blood sampling, platelet counts, and serum thrombopoietin, interleukin-6 and C-reactive protein levels were measured. Platelets did not decrease in patients with sepsis, but they significantly decreased in patients with severe sepsis and septic shock (p <.01 vs. controls and sepsis). In contrast, thrombopoietin levels (median [range]) increased in patients with sepsis (159 [34-1272] pg/mL) compared with controls (57 [33-333] pg/mL, p <.001), exhibiting further significant increase in patients with severe sepsis and septic shock (461 [73-1550] and 522 [45-2313] pg/mL, respectively, p <.001 vs. sepsis). In multiple regression analysis, thrombopoietin levels were independently related only to sepsis severity (higher in patients with increased sepsis severity, p <.001) and platelet counts (higher in patients with lower platelet counts, p =.004). Sepsis severity accounted for most of the variance explained by the model. Thrombopoietin was significantly related to interleukin-6 (r =.26) and C-reactive protein (r =.37, p <.001 for both). In serial measurements, interleukin-6 peak values constantly preceded those of thrombopoietin, whereas peaks in thrombopoietin levels coincided with clinical episodes of septic shock.
  
  
  CONCLUSIONS
  Sepsis severity is the major determinant of elevated thrombopoietin levels in septic patients, whereas platelet count is a secondary determinant. Thrombopoietin represents a potential marker of sepsis severity.
authors.affiliation: Associate Professor of Intensive Care Medicine, University of Athens Medical School, Athens, Greece. szakynthinos@yahoo.com
authors.forename: Spyros G
authors.initials: SG
authors.lastname: Zakynthinos
authors.forename: Spyros
authors.initials: S
authors.lastname: Papanikolaou
authors.forename: Thodoris
authors.initials: T
authors.lastname: Theodoridis
authors.forename: Epaminondas G
authors.initials: EG
authors.lastname: Zakynthinos
authors.forename: Vana
authors.initials: V
authors.lastname: Christopoulou-Kokkinou
authors.forename: George
authors.initials: G
authors.lastname: Katsaris
authors.forename: Antonis C
authors.initials: AC
authors.lastname: Mavrommatis
mesh_terms: D018806:APACHE; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000378:metabolism; D003422:Critical Care; D005260:Female; D006785:Hospitals, University; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008875:Middle Aged; D009102:Multiple Organ Failure / Q000097:blood / Q000209:etiology; D010976:Platelet Count; D011237:Predictive Value of Tests; D011379:Prognosis; D012307:Risk Factors; D012772:Shock, Septic / Q000097:blood* / Q000209:etiology; D018746:Systemic Inflammatory Response Syndrome / Q000097:blood* / Q000209:etiology; D013926:Thrombopoietin / Q000097:blood*
chemical_list: D015415:Biomarkers; D015850:Interleukin-6; D002097:C-Reactive Protein; D013926:Thrombopoietin