[DNA chip analysis of gene expression patterns in poorly-differentiated human stage I lung adenocarcinoma].
OBJECTIVE
To screen genes with abnormal expression in poorly-differentiated human lung adenocarcinoma at stage I with cDNA chip.


METHODS
The mRNA was extracted from cancer tissue and matched normal lung tissue, and was labeled by Cy5-dUTP or Cy3-dUTP as probes. Subsequently, the mixed probes were hybridized to the cDNA chip containing 8192 genes. The information was obtained by managing the cDNA chip with ScanArray4000 scanner and GenePix3.0 software.


RESULTS
Five hundred and eighty genes were differentially expressed between cancer and normal lung tissue. Compared with normal lung tissue, 405 genes were up-regulated and 175 genes were down-regulated in cancer tissue. These genes are involved in different cell activities such as growth regulation and signal transduction. Among the 66 genes with remarkable differential expression between the two tissues, 39 were up-regulated and 27 down-regulated.


CONCLUSIONS
Many different kinds of genes are possibly involved in the initiation and progression of human lung adenocarcinoma. cDNA chip technique might be a useful method in screening lung cancer implicated genes.
Xing
Guo-hong
GH
Respiratory Department of Nanlou, PLA General Hospital, Beijing 100853, China. xinggh90@sina.com
Zhang
Jin-chuan
JC
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D002453:Cell Cycle; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011509:Proteoglycans / Q000235:genetics
C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans