title: The radiotoxicity of 131I therapy of thyroid cancer: assessment by micronucleus assay of B lymphocytes.
abstract: We investigated cytologic radiation damage in thyroid cancer after (131)I therapy using micronucleus assay (MNA) of B lymphocytes exclusively, as opposed to our previous study in which MNA of all lymphocyte subsets was used.
  
  
  METHODS
  We studied 22 thyroid cancer patients treated with 3.7 GBq of (131)I. Peripheral lymphocytes were harvested, and B lymphocytes were isolated by an immunomagnetic method and assayed for the frequency of micronuclei.
  
  
  RESULTS
  The frequency of micronuclei among B cells after (131)I therapy was significantly increased relative to that in untreated control subjects, and the (131)I-induced increase in micronuclei frequency among B cells was significantly greater than that among all lymphocytes.
  
  
  CONCLUSIONS
  Compared with the MNA of all lymphocytes, the MNA among specifically B cells may more sensitively detect cytologic radiation damage associated with (131)I therapy of thyroid cancer.
authors.affiliation: Department of Radiology, Toyama Medical and Pharmaceutical University, Toyama, Japan. nw31456@ms.toyama-mpu.ac.jp
authors.forename: Naoto
authors.initials: N
authors.lastname: Watanabe
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kanegane
authors.forename: Seigo
authors.initials: S
authors.lastname: Kinuya
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Shuke
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Yokoyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kato
authors.forename: Gakuto
authors.initials: G
authors.lastname: Tomizawa
authors.forename: Masashi
authors.initials: M
authors.lastname: Shimizu
authors.forename: Hisashi
authors.initials: H
authors.lastname: Funada
authors.forename: Hikaru
authors.initials: H
authors.lastname: Seto
mesh_terms: D000328:Adult; D000368:Aged; D001402:B-Lymphocytes / Q000473:pathology / Q000528:radiation effects*; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000528:radiation effects; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes / Q000009:adverse effects* / Q000627:therapeutic use*; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D011832:Radiation Injuries / Q000209:etiology* / Q000235:genetics*; D019275:Radiopharmaceuticals / Q000009:adverse effects / Q000627:therapeutic use; D011878:Radiotherapy / Q000009:adverse effects; D013961:Thyroid Gland / Q000473:pathology / Q000528:radiation effects*; D013964:Thyroid Neoplasms / Q000097:blood / Q000473:pathology / Q000532:radiotherapy*
chemical_list: D007457:Iodine Radioisotopes; D019275:Radiopharmaceuticals