title: LRP-1/TbetaR-V mediates TGF-beta1-induced growth inhibition in CHO cells.
abstract: The type V transforming growth factor-beta (TGF-beta) receptor (TbetaR-V) is hypothesized to be involved in cellular growth inhibition by TGF-beta(1). Recently, TbetaR-V was found to be identical to low density lipoprotein receptor-related protein-1 (LRP-1). Here we demonstrate that TGF-beta(1) inhibits growth of wild-type CHO cells but not LRP-1-deficient mutant cells (CHO-LRP-1(-) cells). Stable transfection of CHO-LRP-1(-) cells with LRP-1 cDNA restores the wild-type morphology and the sensitivity to growth inhibition by TGF-beta(1). In addition, overexpression of LRP-1 minireceptors exerts a dominant negative effect and attenuates the growth inhibitory response to TGF-beta(1) in wild-type CHO cells. These results suggest that LRP-1/TbetaR-V is critical for TGF-beta(1)-mediated growth inhibition in CHO cells.
authors.affiliation: Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, 1402 South Grand Boulevard, St. Louis, MO 63104, USA.
authors.forename: Wen-Fang
authors.initials: WF
authors.lastname: Tseng
authors.forename: Shuan Shian
authors.initials: SS
authors.lastname: Huang
authors.forename: Jung San
authors.initials: JS
authors.lastname: Huang
mesh_terms: D000818:Animals; D016466:CHO Cells / Q000502:physiology*; D002455:Cell Division / Q000502:physiology*; D048429:Cell Size; D006224:Cricetinae; D004705:Endocytosis / Q000502:physiology; D026503:Low Density Lipoprotein Receptor-Related Protein-1 / Q000235:genetics / Q000378:metabolism*; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D053773:Transforming Growth Factor beta1
chemical_list: D026503:Low Density Lipoprotein Receptor-Related Protein-1; D017395:Plasminogen Activator Inhibitor 1; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1