Cloning and sequencing of full-length cDNAs of RNA1 and RNA2 of a Tomato black ring virus isolate from Poland.
Full-length cDNA clones corresponding to the RNA1 and RNA2 of the Polish isolate MJ of Tomato black ring virus (TBRV, genus Nepovirus) were obtained using a direct recombination strategy in yeast, and their complete nucleotide sequences were established. RNA1 is 7358 nucleotides and RNA2 is 4633 nucleotides in length, excluding the poly(A) tails. Both RNAs contain a single open reading frame encoding polyproteins of 254 kDa and 149 kDa for RNA1 and RNA2 respectively. Putative cleavage sites were identified, and the relationships between TBRV and related nepoviruses were studied by sequence comparison.
Jończyk
M
M
Department of Virology and Bacteriology, Institute of Plant Protection, Poznań, Poland. m.palczewska@ior.poznan.pl
Le Gall
O
O
Pałucha
A
A
Borodynko
N
N
Pospieszny
H
H
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D017860:Nepovirus / Q000235:genetics*; D016366:Open Reading Frames; D011044:Poland; D020815:Polyproteins / Q000235:genetics; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics; D016415:Sequence Alignment; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics
D018076:DNA, Complementary; D020815:Polyproteins; D012367:RNA, Viral; D014764:Viral Proteins