title: Application of ORAL.screen saliva drug test for the screening of methamphetamine, MDMA, and MDEA incorporated in hair.
abstract: By the use of a one-step immunoassay drug test for oral fluid, a convenient and fairly sensitive screening method has been devised for methamphetamine (MA), 3,4-methylenedioxymethamphetamine (MDMA), and 3,4-methylenedioxyethylamphetamine (MDEA) incorporated in hair. These drugs, in a 10-mg portion of hair, were extracted into 5M HCl/methanol (1:20, v/v), and the extract reconstituted in 100 micro L water was assayed with the saliva drug test ORAL.screen trade mark. The limits of detection were 0.5 ng/mg hair for d-MA, 0.8 ng/mg for dl-MDMA, and 1.0 ng/mg for dl-MDEA. The results are in good agreement with those of gas chromatography-mass spectrometry (GC-MS) determination. Although all positive results must be confirmed by either GC-MS or a specific alternative methodology, this method provided a simple screening, suitable for drug enforcement purposes, while requiring only a 10-mg hair specimen.
authors.affiliation: Forensic Science Laboratory, Osaka Prefectural Police Headquarters, 1-3-18 Hommachi, Chuo-ward, Osaka 541-0053, Japan. CZD13116@nifty.ne.jp
authors.forename: Akihiro
authors.initials: A
authors.lastname: Miki
authors.forename: Munehiro
authors.initials: M
authors.lastname: Katagi
authors.forename: Noriaki
authors.initials: N
authors.lastname: Shima
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Tsuchihashi
mesh_terms: D015104:3,4-Methylenedioxyamphetamine / Q000031:analogs & derivatives* / Q000032:analysis*; D005554:Forensic Medicine / Q000379:methods; D006197:Hair / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay; D007120:Immunochemistry; D008694:Methamphetamine / Q000032:analysis*; D018817:N-Methyl-3,4-methylenedioxyamphetamine / Q000032:analysis*; D011933:Reagent Kits, Diagnostic; D012463:Saliva / Q000737:chemistry; D015813:Substance Abuse Detection / Q000379:methods*
chemical_list: D011933:Reagent Kits, Diagnostic; D008694:Methamphetamine; D015104:3,4-Methylenedioxyamphetamine; D018817:N-Methyl-3,4-methylenedioxyamphetamine; C051800:3,4-methylenedioxyethamphetamine