Binding interaction of gatifloxacin with bovine serum albumin.
The binding of gatifloxacin to bovine serum albumin (BSA) in aqueous solution was studied using fluorescence spectroscopy and absorbance spectra, Further, the interactions influenced by Fe3+ and Cu2+ were also explored in this work. Based on Scatchard's site-binding model and florescence quenching, practical formulas for small molecule ligands to bio-macromolecules have been proposed. The binding parameters were measured according to suggested models, and the binding distance and the transfer efficiency of energy between gatifloxacin and BSA were also obtained in view of the Förster theory of non-radiation energy transfer. The effect of gatifloxacin on the conformation of BSA has also been analyzed using synchronous fluorescence spectroscopy.
Guo
Ming
M
Department of Chemistry, Zhejiang University, HangZhou 310027, China.
Zou
Jian-Wei
JW
Yi
Ping-Gui
PG
Shang
Zhi-Cai
ZC
Hu
Gui-Xiang
GX
Yu
Qing-Sen
QS
D000465:Algorithms; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry*; D001665:Binding Sites; D002417:Cattle; D024841:Fluoroquinolones / Q000737:chemistry*; D000077734:Gatifloxacin; D007202:Indicators and Reagents; D007700:Kinetics; D008670:Metals / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D007202:Indicators and Reagents; D008670:Metals; D012710:Serum Albumin, Bovine; D000077734:Gatifloxacin