title: Actin polymerization-driven molecular movement of mDia1 in living cells.
abstract: mDia1, a Rho effector, belongs to the Formin family of proteins, which shares the conserved tandem FH1-FH2 unit structure. Formins including mDia1 accelerate actin nucleation while interacting with actin filament fast-growing ends. Here our single-molecule imaging revealed fast directional movement of mDia1 FH1-FH2 for tens of microns in living cells. The movement of mDia1 FH1-FH2 was blocked by actin-perturbing drugs, and the speed of mDia1 FH1-FH2 movement appeared to correlate with actin elongation rates. In vitro, mDia1 FH1-FH2 associated persistently with the growing actin barbed end. mDia1 probably moves processively along the growing end of actin filaments in cells, and Formins may be a molecular motility machinery that is independent from motor proteins.
authors.affiliation: Department of Pharmacology, Kyoto University Faculty of Medicine, Yoshida Konoe-cho, Sakyo-ku, Kyoto, Japan.
authors.forename: Chiharu
authors.initials: C
authors.lastname: Higashida
authors.forename: Takushi
authors.initials: T
authors.lastname: Miyoshi
authors.forename: Akiko
authors.initials: A
authors.lastname: Fujita
authors.forename: Fabian
authors.initials: F
authors.lastname: Oceguera-Yanez
authors.forename: James
authors.initials: J
authors.lastname: Monypenny
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Andou
authors.forename: Shuh
authors.initials: S
authors.lastname: Narumiya
authors.forename: Naoki
authors.initials: N
authors.lastname: Watanabe
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D000199:Actins / Q000378:metabolism*; D000818:Animals; D001704:Biopolymers; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000378:metabolism / Q000494:pharmacology; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D015638:Cytochalasin D / Q000378:metabolism / Q000494:pharmacology; D047630:Depsipeptides; D000080865:Formins; D051379:Mice; D008870:Microtubules / Q000187:drug effects / Q000502:physiology; D009068:Movement; D009154:Mutation; D009218:Myosins / Q000502:physiology; D010456:Peptides, Cyclic / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism / Q000494:pharmacology; D053778:Thiazolidines; D014981:Xenopus; D020742:rhoA GTP-Binding Protein / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000199:Actins; D001704:Biopolymers; D019086:Bridged Bicyclo Compounds, Heterocyclic; D002352:Carrier Proteins; D047630:Depsipeptides; C491464:Diap1 protein, mouse; D000080865:Formins; D010456:Peptides, Cyclic; D011993:Recombinant Fusion Proteins; D013844:Thiazoles; D053778:Thiazolidines; C057531:jasplakinolide; D015638:Cytochalasin D; D009218:Myosins; D020742:rhoA GTP-Binding Protein; C037068:latrunculin B