[Hypermethylation of syk gene in promoter region is associated with oncogenesis, metastasis of breast carcinoma].
OBJECTIVE
To investigate the relationship between methylation of syk (spleen tyrosine kinase) gene in promoter region and oncogenesis, metastasis in breast carcinoma.


METHODS
Using RT-PCR technique, specimens from 40 breast cancer patients (tumor tissues, adjacent normal tissues), 15 fibroadenoma were detected for their expression of the syk gene. Meanwhile, Methylation-specific PCR was used to detect methylation of syk promoter region.


RESULTS
The expression of syk gene were detected in normal breast tissues and fibroadenoma samples, 9 out of 40 breast carcinoma samples were found the expression of syk mRNA, syk expression in breast carcinoma was lower than that in adjacent normal tissues and fibroadenoma samples. No methylation of syk promoter was found in adjacent normal tissues and fibroadenoma samples, 17 cases in 40 breast carcinoma patients (42.5%) were detected hypermethylation of syk gene in promoter region, the rate of methylation of syk promoter in breast carcinoma was higher than that of in adjacent normal tissues (P < 0.05). In 18 cases with lymph node metastasis, 14 were found with syk promoter methylation. A significant difference was noted between two groups (P < 0.05).


CONCLUSIONS
Hypermethylation leads to lose of the syk gene expression in human breast carcinoma. Methylation of syk promoter may be correlated to oncogenesis, metastasis of breast carcinoma.
Ding
Yong-bin
YB
Department of General Surgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Wu
Zheng-yan
ZY
Wang
Shui
S
Zha
Xiao-ming
XM
Zheng
Wei
W
Liu
Xiao-an
XA
Fan
Ping
P
D000293:Adolescent; D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000209:etiology / Q000235:genetics*; D019175:DNA Methylation; D004792:Enzyme Precursors / Q000235:genetics*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000072377:Syk Kinase
D004792:Enzyme Precursors; D047908:Intracellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D011505:Protein-Tyrosine Kinases; C000606896:SYK protein, human; D000072377:Syk Kinase