Photosensitized NADH formation system with multilayer TiO2 film.
A TiO(2)/polymer film on a quartz plate fabricated by a layer-by-layer method was employed for NADH production from NAD(+) with lipoamide dehydrogenase and methyl viologen in Tris-HCl buffer on irradiation with UV light, and the ultra thin film prevented from enzyme deactivating effectively.
Sagawa
Takashi
T
Institute of Advanced Energy, Kyoto University, Gokasho, Uji 611-0011, Japan. t-sagawa@iae.kyoto-u.ac.jp
Sueyoshi
Ryota
R
Kawaguchi
Mikako
M
Kudo
Mayu
M
Ihara
Hirotaka
H
Ohkubo
Katsutoshi
K
D002021:Buffers; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism; D004789:Enzyme Activation; D018625:Microscopy, Atomic Force; D009243:NAD / Q000378:metabolism*; D010269:Paraquat / Q000378:metabolism; D010777:Photochemistry; D011108:Polymers / Q000737:chemistry*; D011791:Quartz / Q000737:chemistry; D014025:Titanium / Q000737:chemistry*; D014466:Ultraviolet Rays
D002021:Buffers; D011108:Polymers; D009243:NAD; D011791:Quartz; C009495:titanium dioxide; D014025:Titanium; D008058:Dihydrolipoamide Dehydrogenase; D010269:Paraquat