title: Influence of a detergent on skin response to methyldibromo glutaronitrile in sensitized individuals.
abstract: The present study was undertaken to evaluate the combined effect of the preservative methyldibromo glutaronitrile (MDBGN) and sodium lauryl sulfate (SLS) on the elicitation response of allergic contact dermatitis. 20 volunteers with contact allergy to MDBGN were patch tested with 5 concentrations (10, 50, 100, 500 and 1000 p.p.m.) of MDBGN alone and in combination with 0.25% SLS on the upper arms for 24 h. Skin reactions were evaluated by clinical scoring, and data were evaluated by logistic dose-response models. Additionally, evaluation of skin reactions was performed by measurement of transepidermal water loss (TEWL) and skin colour. Measurements were obtained at baseline and at D3 and D7. As evaluated by clinical assessment, allergic reactions to MDBGN were elicited at lower concentrations when applied in combination with SLS than when applied alone. The response was augmented by a factor of 6.4. An increased response to combined exposure to SLS and MDBGN as compared with MDBGN alone was confirmed by TEWL and colour measurements. Effects of exposure time and concentration of the detergent are discussed. In conclusion, an augmented response was found after concurrent application of MDBGN and SLS. The response was augmented by a factor of 6.4, with confidence limits of 2.8-14.6 (P < 0.0001). This result is important in relation to the determination of threshold values and the risk assessment of contact allergens in consumer and industrial products, where allergens are often present in combination with surfactants.
authors.affiliation: The National Allergy Research Centre, University of Copenhagen, Hellerup, Denmark. linpe@gentoftehosp.kbhamt.dk
authors.forename: Line Kynemund
authors.initials: LK
authors.lastname: Pedersen
authors.forename: Pia
authors.initials: P
authors.lastname: Haslund
authors.forename: Jeanne Duus
authors.initials: JD
authors.lastname: Johansen
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Held
authors.forename: Aage
authors.initials: A
authors.lastname: Vølund
authors.forename: Tove
authors.initials: T
authors.lastname: Agner
mesh_terms: D000485:Allergens / Q000008:administration & dosage / Q000009:adverse effects*; D003124:Colorimetry; D017449:Dermatitis, Allergic Contact / Q000209:etiology*; D003902:Detergents / Q000008:administration & dosage / Q000009:adverse effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D016015:Logistic Models; D008297:Male; D009570:Nitriles / Q000008:administration & dosage / Q000009:adverse effects*; D010328:Patch Tests; D011310:Preservatives, Pharmaceutical / Q000008:administration & dosage / Q000009:adverse effects*; D012967:Sodium Dodecyl Sulfate / Q000008:administration & dosage / Q000009:adverse effects*; D013997:Time Factors
chemical_list: D000485:Allergens; D003902:Detergents; D009570:Nitriles; D011310:Preservatives, Pharmaceutical; D012967:Sodium Dodecyl Sulfate; C039411:1,2-dibromo-2,4-dicyanobutane