Absence of cross-reactivity between murine Ly-6C and Ly-6G.
BACKGROUND
The Ly-6 family has many members, including Ly-6C and Ly-6G. A previous study suggested that the anti-Ly-6G antibody, RB6-8C5, may react with Ly-6Chi murine bone marrow (BM) cells. This finding has been interpreted as cross-reactivity of RB6-8C5 with the Ly-6C antigen, and has been generalized to many hematopoietic cell types, using the terminology Ly-6G/C. The present study was undertaken to determine whether anti-Ly-6G antibodies truly cross-react with the Ly-6C antigen on multiple hematopoietic cell types.


METHODS
Splenocytes, thymocytes, and BM cells obtained from Ly-6.1 and Ly-6.2 strains of mice were stained with a variety of antibodies to Ly-6C and Ly-6G. Flow cytometric analysis was performed on these populations.


RESULTS
Evaluation of anti-Ly-6C and anti-Ly-6G staining showed only Ly-6C expression and no Ly-6G expression on subsets of splenic T and B cells and thymocytes from Ly-6.1 and Ly-6.2 mice. Bone marrow cells were identified that express both Ly-6G and Ly-6C; no Ly-6G+Ly-6C- populations were seen.


CONCLUSIONS
Multiple Ly-6C+ hematopoietic cell populations were identified that do not stain with anti-Ly-6G antibodies. This calls into question the use of the Ly-6G/C nomenclature and suggests that epitopes recognized by anti-Ly-6G antibodies should simply be designated Ly-6G.
Nagendra
Sanjai
S
Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA.
Schlueter
Annette J
AJ
D000818:Animals; D000950:Antigens, Ly / Q000276:immunology* / Q000378:metabolism; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D003429:Cross Reactions / Q000276:immunology*; D004247:DNA / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D009838:Oligodeoxyribonucleotides; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism
D000950:Antigens, Ly; D015704:CD4 Antigens; D016827:CD8 Antigens; C423449:CpG ODN 1826; C494192:Ly-6C antigen, mouse; C480309:Ly6G antigen, mouse; D009838:Oligodeoxyribonucleotides; D004247:DNA