Reactivation of porcine cytomegalovirus through allogeneic stimulation.
Reactivation of latent porcine cytomegalovirus after coculture of peripheral blood mononuclear cells (PBMCs) from pigs with different genetic backgrounds was investigated. Nine of 10 allogeneic coculture pairs were PCR (DNA) positive, whereas 7 coculture pairs had porcine cytomegalovirus (PCMV) RNA, an indication of virus replication. The cell subpopulations harboring PCMV were monocytes and CD8+ T cells.
Guedes
Maria Isabel M C
MI
Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota 55108, USA.
Risdahl
Jack M
JM
Wiseman
Barry
B
Molitor
Thomas W
TW
D000818:Animals; D002478:Cells, Cultured; D018920:Coculture Techniques; D003587:Cytomegalovirus / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D007963:Leukocytes, Mononuclear / Q000502:physiology / Q000821:virology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine / Q000821:virology*; D014184:Transplantation, Homologous; D014775:Virus Activation; D017735:Virus Latency
D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral