In vivo genotoxic effect of nickel chloride in mice leukocytes using comet assay.
DNA damage induced by nickel chloride (NiCl2) in leucocytes of Swiss albino mice has been studied in vivo. The comet assay or the alkaline single cell gel electrophoresis (SCGE) assay was used to measure the DNA damage. The mice were administered orally with acute doses of 3.4, 6.8, 13.6, 27.2, 54.4 and 108.8 mg/kg body weight (b.wt.) NiCl2. Samples of whole blood were collected at 24, 48 and 72 h, first week and second week post-treatment for alkaline SCGE assay to study single/double strand breaks in DNA. A significant increase in mean comet tail length indicating DNA damage was observed with NiCl2 at 24, 48 and 72 h post-treatment (P<0.05). A gradual decrease in the mean tail length was observed at 72 h post-treatment indicating repair of the damaged DNA. The mean tail length showed a dose-related increase and time dependent decrease after treatment with NiCl2 when compared to controls. The study also confirms that the comet assay is a sensitive and rapid method to detect DNA damage caused by heavy metals like nickel (Ni).
Danadevi
K
K
Toxicology Unit, Biology Division, Indian Institute of Chemical Technology, Hyderabad-500 007, Andhra Pradesh, India.
Rozati
R
R
Saleha Banu
B
B
Grover
P
P
D000284:Administration, Oral; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay / Q000379:methods*; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D007962:Leukocytes / Q000187:drug effects*; D008297:Male; D051379:Mice; D009532:Nickel / Q000633:toxicity*; D011897:Random Allocation; D012680:Sensitivity and Specificity; D013997:Time Factors
C022838:nickel chloride; D009532:Nickel; D004247:DNA