title: A new model for human intestinal preservation: comparison of University of Wisconsin and Celsior preservation solutions.
abstract: OBJECTIVE
  We compared University of Wisconsin (UW) and Celsior preservation solutions using a new model of human intestinal preservation that mimics the clinical conditions of small bowel procurement.
  
  
  METHODS
  Intestinal grafts were harvested from four multiorgan donors. After classic warm dissection for organ procurement, an ileal segment of 50 cm was immediately flushed with Celsior. After the perfusion of the abdominal organs with UW, a second segment of adjacent ileum was harvested. The two intestinal grafts were then divided into segments by stapling, before immersion into the corresponding preservation solution (Celsior or UW) for 0-, 6-, 12-, or 24-hour incubation at 4 degrees C. A histological score was graded after blinded examination of three random specimens within each ileal graft for each duration of preservation.
  
  
  RESULTS
  Control specimens showed normal histology. After 6 hours of preservation, most villi showed complete epithelial detachment although the crypts appeared intact. After 12 hours of preservation, a larger proportion of the villi showed extensive epithelial sloughing. After 24 hours, the damage involved the entire mucosa with the crypt epithelium largely detached from the basal membrane. No statistical difference in histological score was observed between the two preservation solutions.
  
  
  CONCLUSIONS
  This study showed severe histological alterations of graft mucosa after short periods of preservation by UW or Celsior solutions. This model may be useful to evaluate improvements in the quality of preservation of human intestinal transplants.
authors.affiliation: Departments of Transplantation and Pathology, Centre Hospitalier Universitaire, Liège, Belgium. A.DeRoover@chu.ulg.ac.be
authors.forename: A
authors.initials: A
authors.lastname: deRoover
authors.forename: L
authors.initials: L
authors.lastname: de Leval
authors.forename: J
authors.initials: J
authors.lastname: Gilmaire
authors.forename: O
authors.initials: O
authors.lastname: Detry
authors.forename: J
authors.initials: J
authors.lastname: Boniver
authors.forename: P
authors.initials: P
authors.lastname: Honoré
authors.forename: M
authors.initials: M
authors.lastname: Meurisse
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; D005978:Glutathione; D006639:Histidine; D006801:Humans; D007082:Ileum; D007328:Insulin; D007413:Intestinal Mucosa / Q000166:cytology; D008353:Mannitol; D008871:Microvilli / Q000648:ultrastructure; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D011887:Raffinose
chemical_list: C088453:Celsior; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D008353:Mannitol; D006639:Histidine; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose