Differences in preparative loadability between the charged and uncharged forms of ionizable compounds.
Ionizable compounds experience a drastic difference in preparative loadability as a function of pH. It can be shown that the preparative loadability of a compound in the ionic form is by a factor of 20 or more inferior to the loadability of the same compound in the unionized form. In this paper, we demonstrate the reason for this behavior, and show practical applications of the principle.
Neue
Uwe Dieter
UD
Waters Corporation, 34 Maple Street, Milford, MA 01757, USA. uwe_neue@waters.com
Wheat
Thomas E
TE
Mazzeo
Jeffrey R
JR
Mazza
Cecilia B
CB
Cavanaugh
Jie Y
JY
Xia
Fang
F
Diehl
Diane M
DM
D000327:Adsorption; D006863:Hydrogen-Ion Concentration; D007477:Ions
D007477:Ions