Field evaluation of a novel colorimetric method--double-site enzyme-linked lactate dehydrogenase immunodetection assay--to determine drug susceptibilities of Plasmodium falciparum clinical isolates from northwestern Thailand.
A double-site enzyme-linked lactate dehydrogenase enzyme immunodetection assay was tested against field isolates of Plasmodium falciparum for assessing in vitro drug susceptibilities to a wide range of antimalarial drugs. Its sensitivity allowed the use of parasite densities as low as 200 parasites/microl of blood. Being a nonisotopic, colorimetric assay, it lies within the capabilities of a modest laboratory at the district level.
Brockman
Alan
A
Shoklo Malaria Research Unit, Mae Sot, Tak 63110, Thailand. alan.brockman@menzies.edu.au
Singlam
Sittaporn
S
Phiaphun
Lucy
L
Looareesuwan
Sornchai
S
White
Nicholas J
NJ
Nosten
François
F
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D003124:Colorimetry; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000737:chemistry*; D016778:Malaria, Falciparum / Q000469:parasitology*; D017711:Nonlinear Dynamics; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000201:enzymology*; D013785:Thailand
D000962:Antimalarials; D007770:L-Lactate Dehydrogenase