Multiplex PCR for diagnosis of enteric infections associated with diarrheagenic Escherichia coli.
A multiplex PCR for detection of three categories of diarrheagenic Escherichia coli was developed. With this method, enterohemorrhagic E. coli, enteropathogenic E. coli, and enterotoxigenic E. coli were identified in fecal samples from patients with hemorrhagic colitis, watery diarrhea, or hemolytic-uremic syndrome and from food-borne outbreaks.
Vidal
Roberto
R
Programa de Microbiologia, Institode Ciencias Biomedicas, Facultad de Medicina, Universidad de Chile, Santiaago. rvidal@machi.med.uchile.cl
Vidal
Maricel
M
Lagos
Rossana
R
Levine
Myron
M
Prado
Valeria
V
D003092:Colitis / Q000175:diagnosis / Q000382:microbiology; D003967:Diarrhea / Q000175:diagnosis / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000382:microbiology; D019453:Escherichia coli O157 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D029968:Escherichia coli Proteins / Q000235:genetics; D005243:Feces / Q000382:microbiology; D005517:Foodborne Diseases / Q000175:diagnosis / Q000382:microbiology; D006463:Hemolytic-Uremic Syndrome / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D029968:Escherichia coli Proteins