title: The membrane topology of EmrE - a small multidrug transporter from Escherichia coli.
abstract: EmrE is a multidrug transporter from Escherichia coli that belongs to the Smr family of small multidrug transporters. The secondary structure of EmrE consists of a four helical bundle, as judged by different techniques. EmrE has been extensively characterized; nevertheless, the membrane topology of EmrE has not been determined yet. Previous work with a homologous Smr protein provided partial information of the membrane topology, however the location of the carboxy-terminus remained inconclusive. In this work we probed the membrane topology of EmrE, focusing on the carboxy-terminus of the protein, using two independent approaches. Our results support a secondary structure where the carboxy-terminus faces the cytoplasm, while the first loop faces the periplasm.
authors.affiliation: Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel.
authors.forename: Shira
authors.initials: S
authors.lastname: Ninio
authors.forename: Yael
authors.initials: Y
authors.lastname: Elbaz
authors.forename: Shimon
authors.initials: S
authors.lastname: Schuldiner
mesh_terms: D000595:Amino Acid Sequence; D017920:Antiporters / Q000737:chemistry* / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D017920:Antiporters; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D013438:Sulfhydryl Compounds; C075436:EmrE protein, E coli