Identification and characterization of gp65, a salivary-gland-specific molecule expressed in the malaria vector Anopheles albimanus.
A group of salivary-gland-specific proteins, designated gp65, were identified in the mosquito Anopheles albimanus. Two-dimensional gel electrophoresis resolved this group into at least four molecules with pI 6.4-6.5. The N-terminal amino acid sequence was determined for the major species, gp65-1, and degenerate oligonucleotide primers were used to amplify a specific probe for library screening. A 1312 bp cDNA clone encoding a predicted translation product of 386 amino acids was recovered. gp65-1 is expressed abundantly in the medial and distal-lateral lobes of the adult female glands, and is secreted in the saliva. The amino acid sequence has potential sites for N-glycosylation, phosphorylation and myristylation, and is similar to a number of proteins of unknown function from other mosquito species.
Montero-Solis
C
C
Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av. Instituto Politécnico Nacional 2508, Col. San Pedro Zacatenco, México D.F., México.
Gonzalez-Ceron
L
L
Rodriguez
M H
MH
Cirerol
B E
BE
Zamudio
F
F
Possanni
L D
LD
James
A A
AA
de la Cruz Hernandez-Hernandez
F
F
D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015723:Gene Library; D015151:Immunoblotting; D008969:Molecular Sequence Data; D012463:Saliva / Q000737:chemistry; D012469:Salivary Glands / Q000033:anatomy & histology / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D012727:Sex Characteristics
D017931:DNA Primers; D012471:Salivary Proteins and Peptides