Effect of disodium cromoglycate on mast cell-mediated immediate-type allergic reactions.
We investigated the effect of disodium cromoglycate (DSCG) on mast cell-mediated immediate-type hypersensitivity. DSCG inhibited systemic allergic reaction induced by compound 48/80 dose-dependently. Passive cutaneous anaphylaxis was inhibited by 71.6% by oral administration of DSCG (1 g/kg). When DSCG was pretreated at concentration rang from 0.01-1000 g/kg, the serum histamine levels were reduced in a dose dependent manner. DSCG also significantly inhibited histamine release from rat peritoneal mast cell (RPMC) by compound 48/80. We confirmed that DSCG inhibited compound 48/80-induced degranulation of RPMC by alcian blue/nuclear fast red staining. In addition, DSCG showed a significant inhibitory effect on anti-dinitrophenyl IgE-mediated tumor necrosis factor-alpha production. These results indicate that DSCG inhibits mast cell-mediated immediate-type allergic reaction.
Shin
Hye-Young
HY
College of Pharmacy, VestibuloCochlear Research Center of Wonkwang University, Iksan, Jeonbuk 570-749, South Korea.
Kim
Jung-Sook
JS
An
Nyeon-Hyoung
NH
Park
Rae-Kil
RK
Kim
Hyung-Min
HM
D000284:Administration, Oral; D000707:Anaphylaxis / Q000139:chemically induced / Q000401:mortality / Q000517:prevention & control*; D000818:Animals; D018927:Anti-Asthmatic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D015550:Cell Degranulation / Q000187:drug effects; D004205:Cromolyn Sodium / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006636:Histamine Release / Q000187:drug effects*; D008136:Longevity / Q000187:drug effects; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D051379:Mice; D008813:Mice, Inbred ICR; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects / Q000276:immunology; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015996:Survival Rate; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D018927:Anti-Asthmatic Agents; D014409:Tumor Necrosis Factor-alpha; D003189:p-Methoxy-N-methylphenethylamine; D004205:Cromolyn Sodium