title: Interleukin-1 beta (IL-1beta) induces tumor necrosis factor alpha (TNF-alpha) expression on mouse myeloid multipotent cell line 32D cl3 and inhibits their proliferation.
abstract: Interleukin-1 alpha (IL-1alpha) and beta (IL-1beta) are well known factors that stimulate hematopoiesis, nevertheless there are reports that show that they can also inhibit this activity. While both IL-1alpha and IL-1beta induce the expression of hematopoietic cytokines, such as growth factors and their receptors on myeloid cells, helping thus to regulate hematopoiesis, it is not known if their inhibitory activity is also mediated through the induction of other specific cytokines. In this work we show that recombinant human IL-1beta (rhIL-1beta) inhibits the proliferation of a mouse IL-3-dependent myeloid multipotent cell line (32D cl3), without inducing its differentiation. We show that rhIL-1beta induces in 32D cl3 cells the expression of the tumor necrosis factor alpha (TNF-alpha) gene, a well known growth inhibitor, and that the rhIL-1beta growth inhibition property on 32D cl3 cells is partially due to this secreted TNF-alpha, hinting thus that the inhibition of hematopoiesis by IL-1 is mediated through other induced cytokines.
authors.affiliation: Laboratorio de Biología Celular y Molecular del Cáncer, UIDCC, FES-Zaragoza, UNAM, Mexico.
authors.forename: Edgar
authors.initials: E
authors.lastname: Ledesma
authors.forename: Ignacio
authors.initials: I
authors.lastname: Martínez
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Córdova
authors.forename: Miriam
authors.initials: M
authors.lastname: Rodríguez-Sosa
authors.forename: Alberto
authors.initials: A
authors.lastname: Monroy
authors.forename: Lourdes
authors.initials: L
authors.lastname: Mora
authors.forename: Isabel
authors.initials: I
authors.lastname: Soto
authors.forename: Gerardo
authors.initials: G
authors.lastname: Ramos
authors.forename: Benny
authors.initials: B
authors.lastname: Weiss
authors.forename: Edelmiro
authors.initials: E
authors.lastname: Santiago Osorio
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D002455:Cell Division / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology; D007375:Interleukin-1 / Q000378:metabolism*; D051379:Mice; D022423:Myeloid Cells / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: D000906:Antibodies; D007375:Interleukin-1; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha