[Experimental study on the proliferation and plasticity of neural stem cells in situ in adult rats after cerebral infarction].
OBJECTIVE
To investigate the proliferation and plasticity of neural stem cells in situ in adult rats after cerebral infarction.


METHODS
Cerebral infarction models of rats were made and the dynamic expression of bromodeoxyuridine (BrdU) and BrdU/polysialylated neural cell adhesion molecule (PSA-NCAM) were determined by immunohistochemistry and immunofluorescence staining.


RESULTS
Compared with the controls, the number of BrdU-positive cells in the subventricular zone (SVZ) and hippocampus increased strikingly at day 1 (P < 0.05), reached maximum at day 7, and decreased markedly at day 14, but it was still elevated compared with that of the controls (P < 0.05); The number of BrdU-labeled with PSA-NCAM-positive cells increased strikingly at day 7 (P < 0.05), reached maximum at day 14, and markedly decreased at day 28, but it was still elevated compared with that of the controls (P < 0.05), and was equal to 60% of the number of BrdU-positive cells in the same period.


CONCLUSIONS
Our results indicate that cerebral infarction stimulate the proliferation of inherent neural stem cells in situ and most proliferated neural stem cells represent neural plasticity.
Zhang
Bo
B
Department of Neurosurgery, PUMC Hospital, CAMS and PUMC, Beijing 100730, China. zhangbopumc@163.com
Wang
Ren-zhi
RZ
Li
Gui-lin
GL
Yao
Yong
Y
Dou
Wan-chen
WC
Li
Shi-fang
SF
Tian
Shi-qiang
SQ
Yin
Jian
J
D000818:Animals; D001973:Bromodeoxyuridine; D002455:Cell Division; D002544:Cerebral Infarction / Q000473:pathology*; D006624:Hippocampus / Q000473:pathology*; D008297:Male; D039842:Neural Cell Adhesion Molecule L1; D009473:Neuronal Plasticity; D009474:Neurons / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012794:Sialic Acids; D013234:Stem Cells / Q000473:pathology*
D039842:Neural Cell Adhesion Molecule L1; D012794:Sialic Acids; C103469:polysialyl neural cell adhesion molecule; D001973:Bromodeoxyuridine