title: Wire-induced myocardial ischemia: a novel approach to create myocardial ischemia in rats.
abstract: BACKGROUND
  Animal models are indispensable in order to investigate the mechanism of various diseases and to explore the counter measures for those disease states. Although there are several animal models of ischemic heart diseases, surgical interventions required to create myocardial ischemia sometimes give rise to a problem in the yield of model. This study describes a new technique for inducing myocardial ischemia in rats.
  
  
  RESULTS
  A 0.014-inch guidewire was introduced via the carotid artery and selectively advanced into the coronary arteries under fluoroscopy. Transmural myocardial ischemia was confirmed by ST-segment elevation and by the appearance of left ventricular wall motion abnormalities on the echocardiogram. Reversibility of the wire-induced myocardial ischemia was demonstrated by complete resolution of both ST-segment elevation and wall motion abnormalities after removing the wire.
  
  
  CONCLUSIONS
  Wire-induced myocardial ischemia was reproducible and is less invasive than conventional ischemic models in rats. This method is a powerful and useful tool for the investigation of ischemic heart disease in small animals.
authors.affiliation: Department of Cardiovascular Medicine, Hokkaido University Graduate School of Medicine, Sapporo, Japan. kazurasa@med.hokudai.ac.jp
authors.forename: Kazushi
authors.initials: K
authors.lastname: Urasawa
authors.forename: Hidetsugu
authors.initials: H
authors.lastname: Sakai
authors.forename: Takahiko
authors.initials: T
authors.lastname: Saito
authors.forename: Naotsugu
authors.initials: N
authors.lastname: Oyama
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kaneta
authors.forename: Akira
authors.initials: A
authors.lastname: Kitabatake
mesh_terms: D000818:Animals; D006328:Cardiac Catheterization / Q000295:instrumentation; D003327:Coronary Disease / Q000209:etiology; D004562:Electrocardiography; D004617:Embolism / Q000209:etiology; D008297:Male; D008863:Microspheres; D023421:Models, Animal; D017202:Myocardial Ischemia / Q000000981:diagnostic imaging / Q000209:etiology* / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012685:Sepharose / Q000008:administration & dosage / Q000633:toxicity; D014463:Ultrasonography
chemical_list: D012685:Sepharose