title: N-bromosuccinimide-fluorescein based sensitive flow-injection chemiluminescence determination of phenformin.
abstract: A novel and highly sensitive method for the determination of phenformin over the range of 6 x 10(-9) - 1 x 10(-5) g ml(-1) in pharmaceutical formulations with flow-injection chemiluminescence (CL) detection is proposed. The method is based on the CL produced during the oxidation of N-bromosuccinimide (NBS) in an alkaline medium in the presence of fluorescein as an effective energy transfer agent. The use of cetyltrimethylammonium bromide (CTAB) as a sensitizer enhances the signal magnitude by about 100 times. The detection limit is 2 x 10(-9) g ml(-1) (3sigma) with a relative standard deviation of 2.3% (n = 11) at 1 x 10(-7) g ml(-1) phenformin. Ninety samples can be determined per hour. The method was evaluated by carrying out a recovery study and by the analysis of commercial formulations. The obtained results compared well with those by an official method, and demonstrated good accuracy and precision. The possible CL mechanism of the proposed system was also briefly analyzed.
authors.affiliation: Department of Chemistry, Analytical Science Institute, Southwest China Normal University, Chongqing, 400715, PR China.
authors.forename: Zhouping
authors.initials: Z
authors.lastname: Wang
authors.forename: Zhujun
authors.initials: Z
authors.lastname: Zhang
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Fu
authors.forename: Luqiu
authors.initials: L
authors.lastname: Fang
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhang
mesh_terms: D001974:Bromosuccinimide / Q000737:chemistry*; D017022:Flow Injection Analysis; D019793:Fluorescein / Q000737:chemistry*; D007004:Hypoglycemic Agents / Q000032:analysis*; D049449:Luminescence; D010629:Phenformin / Q000032:analysis*; D012680:Sensitivity and Specificity; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D007004:Hypoglycemic Agents; D013501:Surface-Active Agents; D010629:Phenformin; D001974:Bromosuccinimide; D019793:Fluorescein