Tropomyosin and troponin are required for ovarian contraction in the Caenorhabditis elegans reproductive system.
Ovulation in the nematode Caenorhabditis elegans is coordinated by interactions between the somatic gonad and germ cells. Myoepithelial sheath cells of the proximal ovary are smooth muscle-like cells, but the regulatory mechanism of their contraction is unknown. We show that contraction of the ovarian muscle requires tropomyosin and troponin, which are generally major actin-linked regulators of contraction of striated muscle. RNA interference of tropomyosin or troponin C caused sterility by inhibiting ovarian contraction that is required for expelling mature oocytes into the spermatheca where fertilization takes place, thus causing accumulation of endomitotic oocytes in the ovary. Tropomyosin and troponin C were associated with actin filaments in the myoepithelial sheath, and the association of troponin C with actin was dependent on tropomyosin. A mutation in the actin depolymerizing factor/cofilin gene suppressed the ovulation defects by RNA interference of tropomyosin or troponin C. These results strongly suggest that tropomyosin and troponin are the actin-linked regulators for contraction of ovarian muscle in the C. elegans reproductive system.
Ono
Kanako
K
Department of Pathology, Emory University, Atlanta, Georgia 30322, USA.
Ono
Shoichiro
S
D008841:Actin Cytoskeleton / Q000378:metabolism; D051339:Actin Depolymerizing Factors; D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology*; D004261:DNA Replication; D051345:Destrin; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D010053:Ovary / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D010060:Ovulation; D034622:RNA Interference; D014335:Tropomyosin / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D019209:Troponin C / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*
D051339:Actin Depolymerizing Factors; D051345:Destrin; D008840:Microfilament Proteins; D014335:Tropomyosin; D019209:Troponin C