title: [A new rat model of transplant kidney-sclerosis accelerated by prolonged cold ischemia time].
abstract: OBJECTIVE
  To establish an accelerated animal model of the chronic renal allograft dysfunction in rat.
  
  
  METHODS
  Kidney transplantation was performed from SD to Wistar strain (allogeneic) according to the procedure of Kamada with some modification. Before the transplantation, the kidney was preserved in 0-4 degrees C heparin sodium chloride solution for prolonging the one hour. The serum creatinine level and pathological change of transplant kidney were observed in the 2nd, 4th, 6th, 8th and 12th weeks post-transplantation.
  
  
  RESULTS
  After transplantation, the serum creatinine level of recipient rats obviously increased in the 6th week and the pathologic changes of chronic nephropathy evidently appeared in the 8th week when compared to those of the control group with non-reinforcement I/R injury; a statistically significant difference was noted.
  
  
  CONCLUSIONS
  It is simple and feasible to establish a rat model of (SD-->Wistar) transplant kidney-sclerosis accelerated by prolonging I/R injury.
authors.affiliation: Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Yu-peng
authors.initials: YP
authors.lastname: Xin
authors.forename: Yi-ping
authors.initials: YP
authors.lastname: Lu
authors.forename: Xiu-hui
authors.initials: XH
authors.lastname: Zhang
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Lin
authors.forename: Rui
authors.initials: R
authors.lastname: Gao
authors.forename: Dong-hai
authors.initials: DH
authors.lastname: Teng
mesh_terms: D000818:Animals; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D007511:Ischemia; D007668:Kidney / Q000098:blood supply / Q000473:pathology* / Q000502:physiology; D016030:Kidney Transplantation; D008297:Male; D051381:Rats; D015427:Reperfusion Injury / Q000473:pathology; D012598:Sclerosis
chemical_list: D003404:Creatinine