title: [Pulmonary artery perfusion with hypothermic solution inhibits the apoptosis of lung parenchymal cells during cardiopulmonary bypass].
abstract: OBJECTIVE
  To study effects of pulmonary artery perfusion with hypothermic solution on the apoptosis of lung parenchymal cells during cardiopulmonary bypass.
  
  
  METHODS
  Forty children with tetralogy of Fallot were divided into control group (n = 20) and protective group (n = 20). The patients in control group were performed using routine approaches. Patients' pulmonary artery were infused with 4 degrees C protective solution during cardiopulmonary bypass in protective group. Lung biopsy specimens were obtained after operations in order to study the apoptosis of lung parenchymal cells using tunnel techniques. At same time, patients' pulmonary functions and clinic index were monitored.
  
  
  RESULTS
  The rate of apoptosis cells of lung parenchymal cells was (18 +/- 7)% in control group, whereas (10 +/- 2)% in protective group. There was significant difference between both groups (t = -2.95, P < 0.05). Index O(2) in protective group was higher than that in control group at 0, 6 and 12 hours after operations [(492 +/- 172), (444 +/- 104), (489 +/- 58) mm Hg versus (369 +/- 126), (347 +/- 107), (340 +/- 119) mm Hg, t = 2.59, P < 0.05; t = 2.88, P < 0.01; t = 5.06, P < 0.01, respectively)]. The time of mechanical ventilation was significantly shorter in protective group than in control group [(15 +/- 11) hours versus (26 +/- 15) hours, t = -2.76, P < 0.01].
  
  
  CONCLUSIONS
  Pulmonary artery perfusion with hypothermic solution can inhibit the apoptosis of lung parenchymal cells and relieve cardiopulmonary bypass-induced lung injury.
authors.affiliation: Congenital Heart Disease Center, Fuwai Heart Disease Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100037, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wei
authors.forename: Ying-long
authors.initials: YL
authors.lastname: Liu
authors.forename: Cun-tao
authors.initials: CT
authors.lastname: Yu
authors.forename: Yong-nan
authors.initials: YN
authors.lastname: Chang
authors.forename: Chun-hua
authors.initials: CH
authors.lastname: Li
mesh_terms: D017209:Apoptosis; D002315:Cardiopulmonary Bypass; D016022:Case-Control Studies; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007036:Hypothermia, Induced / Q000379:methods*; D007223:Infant; D008168:Lung / Q000098:blood supply / Q000473:pathology*; D008297:Male; D010477:Perfusion; D011651:Pulmonary Artery; D013771:Tetralogy of Fallot / Q000601:surgery