title: Association of vitamin D receptor and estrogen receptor-alpha gene polymorphism with peak bone mass and bone size in Chinese women.
abstract: OBJECTIVE
  To investigate if vitamin D receptor (VDR) gene Apa I polymorphism and estrogen receptor-alpha (ER-alpha) gene Pvu II, Xba I polymorphisms are related to bone mineral density (BMD), bone mineral content (BMC) and bone size in premenopausal Chinese women.
  
  
  METHODS
  The VDR Apa I genotype and ER-alpha Pvu II, Xba I genotype were determined by PCR-restriction fragment length polymorphism (RFLP) in 493 unrelated healthy women aged 20-40 years of Han nationality in Shanghai city. BMD (g/cm(2)), BMC (g), and bone areal size (BAS, cm(2) ) at lumbar spine 1-4 (L(1-4)) and proximal femur (femoral neck, trochanter and Ward's triangle) were measured by duel-energy X-ray absorptionmetry.
  
  
  RESULTS
  All allele frequencies did not deviate from Hardy-Weinberg equilibrium. After phenotypes were adjusted for age, height, and weight, a significant association was found between VDR Apa I genotype and BMC variation at L(1-4) and Ward's triangle (P<0.05), but not in BMD or BAS at lumbar spine and proximal femur. ER-a Pvu II, Xba I genotype was not related to BMC, BMD, and BAS at all sites.
  
  
  CONCLUSIONS
  The study suggested that Apa I polymorphism in VDR gene may influence on attainment and maintenance of peak bone mass in premenopausal Chinese women.
authors.affiliation: Center for Preventing and Treating Osteoporosis, Osteoporosis Research Unit, Shanghai JiaoTong University Affiliated Sixth People s Hospital, Shanghai 200233, China. yuejuanQ@medmail.com.cn
authors.forename: Yue-juan
authors.initials: YJ
authors.lastname: Qin
authors.forename: Zhen-lin
authors.initials: ZL
authors.lastname: Zhang
authors.forename: Qi-ren
authors.initials: QR
authors.lastname: Huang
authors.forename: Jin-mei
authors.initials: JM
authors.lastname: He
authors.forename: Yun-qiu
authors.initials: YQ
authors.lastname: Hu
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhao
authors.forename: Jing-hui
authors.initials: JH
authors.lastname: Lu
authors.forename: Miao
authors.initials: M
authors.lastname: Li
authors.forename: Yu-juan
authors.initials: YJ
authors.lastname: Liu
mesh_terms: D000328:Adult; D044466:Asian People; D015519:Bone Density; D002681:China; D047628:Estrogen Receptor alpha; D005260:Female; D005269:Femur / Q000033:anatomy & histology; D005787:Gene Frequency; D006801:Humans; D008159:Lumbar Vertebrae / Q000033:anatomy & histology; D012150:Polymorphism, Restriction Fragment Length; D017697:Premenopause; D018167:Receptors, Calcitriol / Q000235:genetics*; D011960:Receptors, Estrogen / Q000235:genetics*
chemical_list: D047628:Estrogen Receptor alpha; D018167:Receptors, Calcitriol; D011960:Receptors, Estrogen