title: Liver necrosis and fulminant hepatic failure in rats: protection by oxyanionic form of tungsten.
abstract: The hepatic lesion produced as a result of oxidative stress is of wide occurrence. In the present study, the effect of tungsten on liver necrosis and fulminant hepatic failure (FHF) has been studied in rats treated with various compounds known to produce oxidative stress. Supplementation of animals with sodium tungstate for 7 weeks before the induction of liver injury by chemicals including thioacetamide (TAA), carbon tetrachloride (CCl(4)), or chloroform (CHCl(3)) could protect progression of hepatic injury. Various biochemical changes associated with liver damage and oxidative stress were measured. Hepatic malondialdehyde content, endogenous tripeptide, and reduced glutathione were measured as oxidative stress markers. The activity of xanthine oxidase, which generates reactive oxygen species (ROS) as a by-product, was also determined and found to be perturbed. Tungsten supplementation to rats caused a significant decrease in lipid peroxidation and lowered the levels of the biochemical markers of hepatic lesions produced by TAA, CCl(4) (CCl(4)), or CHCl(3). Tungsten could also cause an increase in the survival rate in rats receiving lethal doses of TAA, CCl(4), or CHCl(3). The protective effect of tungsten, however, is suggested to be limited to the conditions where the hepatic lesion is reported to be due to the generation of ROS. The progression of liver injury produced by the compounds causing oxidative stress without initiating the generation of free radicals such as bromobenzene (BB), or acetaminophen (AAP), could not be inhibited by tungsten. The possible mechanism explaining the role of oxyanionic form of tungsten in free radical-induced hepatic lesions is discussed.
authors.affiliation: Department of Biochemistry, Faculty of Science, Hamdard University, Hamdard Nagar, New Delhi 110062, India.
authors.forename: Sonica
authors.initials: S
authors.lastname: Pawa
authors.forename: Shakir
authors.initials: S
authors.lastname: Ali
mesh_terms: D000082:Acetaminophen / Q000633:toxicity; D000469:Alkaline Phosphatase / Q000097:blood; D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D001969:Bromobenzenes / Q000633:toxicity; D002252:Carbon Tetrachloride Poisoning / Q000473:pathology*; D002725:Chloroform / Q000633:toxicity; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005260:Female; D007928:Lethal Dose 50; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D017093:Liver Failure / Q000517:prevention & control*; D009336:Necrosis; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013853:Thioacetamide / Q000633:toxicity; D000637:Transaminases / Q000097:blood; D017973:Tungsten Compounds / Q000627:therapeutic use*
chemical_list: D001969:Bromobenzenes; D017973:Tungsten Compounds; D013853:Thioacetamide; D000082:Acetaminophen; D000493:Allopurinol; C025399:sodium tungstate(VI); D002725:Chloroform; C032036:bromobenzene; D000637:Transaminases; D000469:Alkaline Phosphatase; D004121:Dimethyl Sulfoxide