[Cloning the gene fragment coding the putative integrase-like protein from X maltophilia].
OBJECTIVE
To amplify the nucleotide sequence of CG-like receptor from X anthomonas maltophilia(X maltophilia).


METHODS
Using the specific primer P1 designed by Grover's reported 342 bp partial nucleotide sequence of X maltophilia CG-like receptor and random primer to PCR amplify, PCR product was cloned in the pUCm-T vector. After the recombinant plasmid was tested by restriction endonuclease digestion, the insert on the recombinant plasmid was sequenced and analyzed.


RESULTS
About 500 bp PCR product was cloned in the pUCm-T vector and obtained the recombinant pUCm-Int. By sequencing to the insert on the pUCm-Int with M13 universal sequencing primers, the 410-486 bp fragment of the cloned 510 bp nucleotide sequence (GenBank accession number: AY363962) showed 84% identity with the 9304-8958 bp fragment of the XACb0009 gene on plasmid pXAC64 of Xanthomonas axonopodis pv. citri. And the 4-166aa fragment of its translated 169aa sequence had 62% identity with the 38-200aa sequence of integrase-like protein coded by the XACb0009 gene.


CONCLUSIONS
The cloned 510 bp nucleotide sequence was possibly the partial gene sequence coding the integrase-like protein of X maltophilia.
Liang
Shu-fang
SF
Biochemistry Department, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Chen
Man-ling
ML
Zhai
Chao-yang
CY
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D019426:Integrases / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D017422:Sequence Analysis, DNA; D014974:Xanthomonas / Q000201:enzymology / Q000235:genetics*
D004269:DNA, Bacterial; D011994:Recombinant Proteins; D019426:Integrases