title: Elevated antibody levels against Chlamydia pneumoniae, human HSP60 and mycobacterial HSP65 are independent risk factors in myocardial infarction and ischaemic heart disease.
abstract: The relative significance of traditional risk factors, chronic infections and autoimmune processes in the development of acute myocardial infarction (AMI) has not been fully elucidated. We compared serum IgG antibody titres to various pathogens, i.e. Chlamydia pneumoniae (Cpn), cytomegalovirus (CMV) and herpes simplex virus type 1 (HSV-1), and to the potential autoantigens human heat shock protein 60 (hHSP60) and mycobacterial heat shock protein 65 (mHSP65), in serum samples obtained from patients 3-48 h after AMI (n = 40) or stable effort angina (SEA, n = 43), and from controls (n = 46). The strongest association was observed between AMI and the elevated level of hHSP60 antibodies. The association between AMI and the level of Cpn antibodies was also significant. High levels of hHSP60 and Cpn antibodies represented independent risk factors for the development of AMI, but the simultaneous presence of high levels of antibodies to Cpn and hHSP60 suggested a joint effect on the relative risk of AMI (OR = 12.0-21.1). The antibody titres to mHSP65 were higher in the SEA group than in the controls, and the simultaneous presence of high levels of Cpn and mHSP65 antibodies meant an increased risk among the SEA patients. The antibody titres to CMV or HSV-1 were similar in the three groups. In conclusion, these results demonstrate associations of AMI with high levels of anti-hHSP60 and anti-Cpn antibodies, and of SEA with the level of anti-mHSP65 antibodies, these being independent risk factors.
authors.affiliation: Department of Cardiology, Railway Hospital, Budapest, Hungary.
authors.forename: Krisztina
authors.initials: K
authors.lastname: Heltai
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Kis
authors.forename: Katalin
authors.initials: K
authors.lastname: Burian
authors.forename: Valeria
authors.initials: V
authors.lastname: Endresz
authors.forename: Amarilla
authors.initials: A
authors.lastname: Veres
authors.forename: Endre
authors.initials: E
authors.lastname: Ludwig
authors.forename: Eva
authors.initials: E
authors.lastname: Gönczöl
authors.forename: Istvan
authors.initials: I
authors.lastname: Valyi-Nagy
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D016022:Case-Control Studies; D018834:Chaperonin 60 / Q000097:blood*; D016993:Chlamydophila pneumoniae / Q000276:immunology*; D015331:Cohort Studies; D016001:Confidence Intervals; D005260:Female; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D009161:Mycobacterium / Q000276:immunology*; D009203:Myocardial Infarction / Q000453:epidemiology / Q000276:immunology* / Q000382:microbiology; D017202:Myocardial Ischemia / Q000453:epidemiology / Q000276:immunology / Q000382:microbiology; D016017:Odds Ratio; D011336:Probability; D011379:Prognosis; D012016:Reference Values; D018570:Risk Assessment; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial; D015415:Biomarkers; D018834:Chaperonin 60