title: Excretory/secretory products from plerocercoids of Spirometra erinaceieuropaei suppress interleukin-1beta gene expression in murine macrophages.
abstract: The present study shows that ES products from plerocercoids of Spirometra erinaceieuropaei suppressed interleukin-1beta mRNA expression in lipopolysaccharide-stimulated RAW 264.7 macrophages in the absence or presence of a cyclic AMP analogue, dibutyryl cyclic AMP. Investigation using the inhibitors of mitogen-activated protein kinase (MAPK) pathways revealed that extracellular signal-regulated protein kinase 1/2 and p38 mitogen-activated protein kinase pathways are crucial for full induction of interleukin-1beta mRNA expression. ES products additionally suppressed interleukin-1beta mRNA expression in the cells treated with p38 mitogen-activated protein kinase inhibitor (SB203580) or extracellular signal-regulated protein kinase 1/2 inhibitor (PD98059). Western blot analysis showed that dibutyryl cyclic AMP enhanced lipopolysaccharide-induced phosphorylation of extracellular signal-regulated protein kinase 1/2, p38 mitogen-activated protein kinase and cyclic AMP responsive element binding protein (CREB) and, in turn, we demonstrated that ES products reduced the lipopolysaccharide and dibutyryl cyclic AMP-induced phosphorylation of extracellular signal-regulated protein kinase 1/2 and p38 mitogen-activated protein kinase, but not cyclic AMP responsive element binding protein. These data demonstrate that ES products from the plerocercoids of S. erinaceieuropaei may evade induction of interleukin-1beta mRNA by inhibiting extracellular signal-regulated protein kinase 1/2 and p38 mitogen-activated protein kinase pathways in lipopolysaccharide and/or dibutyryl cyclic AMP-stimulated macrophages.
authors.affiliation: Division of Molecular Medical Zoology, Department of Microbiology and Pathology, Faculty of Medicine, Tottori University, 86 Nishi-cho, Yonago 683-8503, Japan.
authors.forename: Paramasari
authors.initials: P
authors.lastname: Dirgahayu
authors.forename: Soji
authors.initials: S
authors.lastname: Fukumoto
authors.forename: Sayuri
authors.initials: S
authors.lastname: Tademoto
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Kina
authors.forename: Kazumitsu
authors.initials: K
authors.lastname: Hirai
mesh_terms: D000818:Animals; D015152:Blotting, Northern / Q000379:methods; D015153:Blotting, Western / Q000379:methods; D003994:Bucladesine / Q000235:genetics; D002460:Cell Line; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics; D007375:Interleukin-1 / Q000235:genetics*; D008070:Lipopolysaccharides; D020935:MAP Kinase Signaling System / Q000235:genetics; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics; D010766:Phosphorylation; D018510:RNA, Helminth / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D013146:Spirometra / Q000235:genetics / Q000502:physiology*
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D007375:Interleukin-1; D008070:Lipopolysaccharides; D018510:RNA, Helminth; D012333:RNA, Messenger; D003994:Bucladesine; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases