[Effect of c-kit gene mutation on prognosis of gastrointestinal stromal tumor].
OBJECTIVE
To evaluate the clinical signification of c-kit gene mutation in gastrointestinal stromal tumor (GIST) and examine whether the presence of mutation of c-kit gene is important as a prognostic factor.


METHODS
The c-kit mutation had been detected by PCR-SSCP, DNA sequence, statistical comparison were used for the relationship of c-kit gene mutation and clinical pathology, clinical behavior, recurrence, et al.


RESULTS
The presence of c-kit mutation correlated with tumor size, proliferating cell nuclear antigen index, mitotic cell number, presence of necrosis, microscopic invasion to adjacent tissues, recurrence and distant metastasis. The age, sex, location of tumor, cell type, the presence of hemorrhage, and c-kit expression were independently related to the presence of c-kit mutation.


CONCLUSIONS
The c-kit gene mutation is an important prognostic factor for GIST.
Ma
Da-lie
DL
Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Liu
Xiao-hong
XH
Bai
Chen-guang
CG
Xie
Qiang
Q
Feng
Fei
F
D000328:Adult; D000367:Age Factors; D001483:Base Sequence; D004252:DNA Mutational Analysis; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000235:genetics / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011379:Prognosis; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics*; D012737:Sex Factors; D017154:Stromal Cells / Q000473:pathology*
D019009:Proto-Oncogene Proteins c-kit