title: Lectins induce resistance to proteases and/or mechanical stimulus in all examined cells--including bone marrow mesenchymal stem cells--on various scaffolds.
abstract: Transplantation of bone marrow mesenchymal stem cells (MSC), chondrocytes, osteoblasts, or muscle cells promotes regeneration. However, these cells adhere poorly to some scaffolds--depending upon the scaffold material--and are often damaged by proteases or mechanical stimuli at site of transplantation. We found, however, that MSC, chondrocytes, and osteoblasts--along with some other cells--that were exposed to phaseolus vulgaris erythroagglutinin (PHA-E) or concanavalin A (ConA) increased their adhesion capacity on plastic tissue culture dishes and on plates of hydroxyapatite, titanium and poly-DL-lactic-co-glycolic acid (PLGA), and that these cells, moreover, built up resistance to proteases and/or mechanical stimuli. Thus, lectins may have great potential in tissue engineering and cell therapy.
authors.affiliation: Department of Prosthetic Dentistry, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734-8553, Japan.
authors.forename: Haruki
authors.initials: H
authors.lastname: Nishimura
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nishimura
authors.forename: Ryo
authors.initials: R
authors.lastname: Oda
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Yamanaka
authors.forename: Takehiro
authors.initials: T
authors.lastname: Matsubara
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Ozaki
authors.forename: Kensuke
authors.initials: K
authors.lastname: Sekiya
authors.forename: Taizo
authors.initials: T
authors.lastname: Hamada
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kato
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D010450:Endopeptidases / Q000378:metabolism*; D005269:Femur; D006801:Humans; D007085:Ilium; D007700:Kinetics; D037102:Lectins / Q000494:pharmacology*; D045170:Matrix Attachment Regions / Q000502:physiology*; D008648:Mesoderm / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011480:Protease Inhibitors / Q000494:pharmacology; D011817:Rabbits; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
chemical_list: D037102:Lectins; D011480:Protease Inhibitors; D003208:Concanavalin A; D010450:Endopeptidases