Genotyping of enteric adenoviruses by using single-stranded conformation polymorphism analysis and heteroduplex mobility assay.
Single-stranded conformation polymorphism (SSCP) analysis and heteroduplex mobility assays (HMAs) were used to identify and genotype enteric adenoviruses (EAd). The results were compared to those of restriction endonuclease assays, species-specific PCRs, and direct nucleotide sequence analyses. Of the 31 stool samples tested, 15 isolates were identified as EAd and 7 were identified as nonenteric Ad by all methods. An agreement of 100% was found between the SSCP and HMA results.
Soares
Caroline C
CC
Departamento de Virologia, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-590, Brazil.
Volotão
Eduardo M
EM
Albuquerque
Maria Carolina M
MC
Nozawa
Carlos M
CM
Linhares
Rosa Elisa C
RE
Volokhov
Dmitriy
D
Chizhikov
Vladimir
V
Lu
Xiaoyan
X
Erdman
Dean
D
Santos
Norma
N
D000258:Adenovirus Infections, Human / Q000821:virology; D000260:Adenoviruses, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001938:Brazil; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis; D003967:Diarrhea / Q000821:virology*; D005243:Feces / Q000821:virology; D005838:Genotype; D020180:Heteroduplex Analysis / Q000379:methods*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
D004279:DNA, Viral