title: Amplification of the cyclin D1 gene is associated with tumour subsite, DNA non-diploidy and high S-phase fraction in squamous cell carcinoma of the head and neck.
abstract: Amplification of CCND1 (cyclin D1 gene) in squamous cell carcinoma of the head and neck (SCCHN) is correlated to poor prognosis. The purpose of this study was to investigate whether CCND1 amplification is related to different subsites and also to DNA ploidy status and S-phase fraction (SPF). Biopsies from 67 patients with SCCHN were analysed for CCND1 amplification by fluorescence in situ hybridisation (FISH) and for ploidy status and SPF by flow cytometry (FCM). Twenty-one of 67 tumours (31%) showed CCND1 amplification and the frequencies differed significantly between different subsites (p = 0.01). Tumours from hypopharynx, larynx and oropharynx showed higher rates of amplification as compared to tumours from oral cavity and epipharynx. CCND1 amplification was also associated to DNA non-diploidy and high SPF (p = 0.002 and p = 0.002, respectively). In conclusion, the rate of CCND1 amplification differed between different subsites in SCCHN and was also associated to a more aggressive tumour phenotype, as defined by DNA non-diploidy and high SPF.
authors.affiliation: Department of Oncology, University Hospital of Lund, SE-22185 Lund, Sweden. emma.nimeus@onk.lu.se
authors.forename: Emma
authors.initials: E
authors.lastname: Niméus
authors.forename: Bo
authors.initials: B
authors.lastname: Baldetorp
authors.forename: Pär-Ola
authors.initials: PO
authors.lastname: Bendahl
authors.forename: Karin
authors.initials: K
authors.lastname: Rennstam
authors.forename: Johan
authors.initials: J
authors.lastname: Wennerberg
authors.forename: Jan
authors.initials: J
authors.lastname: Akervall
authors.forename: Mårten
authors.initials: M
authors.lastname: Fernö
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D005784:Gene Amplification / Q000235:genetics*; D006258:Head and Neck Neoplasms / Q000235:genetics*; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007822:Laryngeal Neoplasms / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics; D009303:Nasopharyngeal Neoplasms / Q000235:genetics; D009959:Oropharyngeal Neoplasms / Q000235:genetics; D010641:Phenotype; D011003:Ploidies; D016196:S Phase / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm