Role of ARF4L in recycling between endosomes and the plasma membrane.
The human ADP-ribosylation factor-like protein, ARF4L is a member of the ARF family, which are small GTP-binding proteins that play significant roles in vesicle transport and protein secretion. However, little is known about the physiological roles of ARF4L. In this study, to understand the biological functions of ARF4L, we carried out immunocytochemical analysis of ARF4L molecules with mutations in the functional domains. ARF4L was shown to be distributed to the plasma membrane following binding to GTP (Q80L), and into endosomes following binding to GDP (T35N). Moreover, the inactive-form of ARF4L (T35N) causes localization of transferrin receptors to the endosomal compartment, while the active form (Q80L) causes transport to the plasma membrane. These findings indicate that ARF4L drive the transport of cargo protein and subsequent fusion of recycling vesicles with the plasma membrane for maintenance of the cell surface.
Katayama
Taiichi
T
Department of Anatony & Neuroscience, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan. katayama@anat2.med.osaka-u.ac.jp
Imaizumi
Kazunori
K
Yoneda
Takunari
T
Taniguchi
Manabu
M
Honda
Akiko
A
Manabe
Takayuki
T
Hitomi
Junichi
J
Oono
Kayoko
K
Baba
Kousuke
K
Miyata
Shingo
S
Matsuzaki
Shinsuke
S
Takatsuji
Koichi
K
Tohyama
Masaya
M
D020727:ADP-Ribosylation Factors / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D004705:Endocytosis / Q000502:physiology*; D011992:Endosomes / Q000378:metabolism* / Q000648:ultrastructure; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D008561:Membrane Fusion / Q000502:physiology; D008854:Microscopy, Electron; D009154:Mutation / Q000235:genetics; D011485:Protein Binding / Q000502:physiology; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000235:genetics; D021381:Protein Transport / Q000502:physiology; D011990:Receptors, Transferrin / Q000378:metabolism; D022161:Transport Vesicles / Q000378:metabolism* / Q000648:ultrastructure
D020033:Protein Isoforms; D011990:Receptors, Transferrin; D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate; D020727:ADP-Ribosylation Factors; C116688:ARF4 protein, human