Molecular study on Y chromosome microdeletions in Egyptian males with idiopathic infertility.
OBJECTIVE
To determine the frequency of genetic deletions within the azoospermia factors in Egyptian infertile males.


METHODS
The Yq microdeletions in 33 infertile males with undetectable chromosomal anomalies were examined by mutiplex polymerase chain reaction (PCR). Deletions were confirmed using single PCR amplifications.


RESULTS
Four out of the total 33 (12 %) men had Yq(11) microdeletions, thus supporting the average reported figures in other populations. Three of those 4 cases had single short tandem sequence deletions with discrete histological findings of their testes. Single sY272 deletion within AZFc was associated with Sertoli cell only syndrome, whereas a patient with isolated sY84 deletion within AZFa had immature testicular structure. The remaining case had a large deletion in AZFa-c and short stature.


CONCLUSIONS
The present study supports the hypothesis that the Yq(11) encompasses genetic determinants of stature besides genes controlling spermatogenesis.
El Awady
Mostafa K
MK
Department of Biomedical Technology, National Research Center, Dokki, Cairo 12622, Egypt. melawady@hotmail.com
El Shater
Sohair F
SF
Ragaa
Ehab
E
Atef
Khaled
K
Shaheen
Ibrahim M
IM
Megiud
Nagwa A
NA
D000328:Adult; D041322:Chromosomes, Human, Y / Q000235:genetics*; D004534:Egypt; D005640:Follicle Stimulating Hormone / Q000097:blood; D017353:Gene Deletion; D006801:Humans; D007248:Infertility, Male / Q000235:genetics* / Q000473:pathology; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D016133:Polymerase Chain Reaction; D012708:Sertoli Cells / Q000473:pathology; D013577:Syndrome; D013737:Testis / Q000473:pathology; D013739:Testosterone / Q000097:blood
D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone