title: Performance of a commercial, type-specific enzyme-linked immunosorbent assay for detection of herpes simplex virus type 2-specific antibodies in Ugandans.
abstract: Two hundred forty-eight human immunodeficiency virus (HIV)-positive and 496 HIV-negative subjects in Uganda were tested by HerpeSelect herpes simplex virus type 2 enzyme-linked immunosorbent assay (ELISA) and Western blotting to optimize the ELISA for use in this population. A higher index cutoff value was required for optimal sensitivity and specificity, and overall performance of the assay was not affected by HIV status.
authors.affiliation: National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Oliver
authors.initials: O
authors.lastname: Laeyendecker
authors.forename: Charla
authors.initials: C
authors.lastname: Henson
authors.forename: Ronald H
authors.initials: RH
authors.lastname: Gray
authors.forename: Ruby H-N
authors.initials: RH
authors.lastname: Nguyen
authors.forename: Bobbi Jo
authors.initials: BJ
authors.lastname: Horne
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Wawer
authors.forename: David
authors.initials: D
authors.lastname: Serwadda
authors.forename: Noah
authors.initials: N
authors.lastname: Kiwanuka
authors.forename: Rhoda Ashley
authors.initials: RA
authors.lastname: Morrow
authors.forename: Wayne
authors.initials: W
authors.lastname: Hogrefe
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Quinn
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D018023:HIV Seronegativity; D006679:HIV Seropositivity / Q000150:complications; D006558:Herpes Genitalis / Q000175:diagnosis*; D018258:Herpesvirus 2, Human / Q000276:immunology*; D006801:Humans; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014454:Uganda
chemical_list: D000914:Antibodies, Viral; D011933:Reagent Kits, Diagnostic