title: [Study of biosensor technology on the detection of endotoxin-neutralizing materials].
abstract: OBJECTIVE
  To explore the application of biosensor technology in the determination of endotoxin-neutralizing materials.
  
  
  METHODS
  After mixing polymyxin B (PMB) with endotoxin in certain concentration, the neutralizing ratio of PMB to endotoxin was assessed by biosensor technique and limulus amebocyte lysate test respectively. The results from the two methods were compared.
  
  
  RESULTS
  The neutralizing ratio of PMB to endotoxin as assessed by biosensor technology was 0.35 microg to 1 ng, while that by dynamic turbidimetric and chromogenic limulus amebocyte lysate (LAL) technique was 0.5 mg to 1 ng and 1 mg to 1 ng, respectively. The results obtained by biotechnology were similar to that by biosensor technique.
  
  
  CONCLUSIONS
  Biosensor technology was an accurate, convenient and rapid method for the determination of potency of endotoxin-neutralizing materials.
authors.affiliation: Institute of Burn Research, Southwestern Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, The Third Military Medical University, Chongqing 400038, P.R. China.
authors.forename: Gen-fa
authors.initials: GF
authors.lastname: Lu
authors.forename: Xiao-yun
authors.initials: XY
authors.lastname: Gong
authors.forename: Guo
authors.initials: G
authors.lastname: Wei
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Guang-xia
authors.initials: GX
authors.lastname: Xiao
authors.forename: Jiang
authors.initials: J
authors.lastname: Zheng
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D004731:Endotoxins / Q000032:analysis*; D008050:Lipid A / Q000032:analysis*; D011112:Polymyxin B / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D001426:Bacterial Proteins; D004731:Endotoxins; D008050:Lipid A; C027315:lipid A-associated protein, Bacteria; D011112:Polymyxin B