title: CCAAT/enhancer-binding protein-beta has a role in osteoblast proliferation and differentiation.
abstract: CCAAT/enhancer binding protein beta (C/EBPbeta) is known to play an important role in the expression of several genes necessary for bone development and homeostasis including osteocalcin, IGF-1, and IL-6. In this study, we show that C/EBPbeta protein levels and, consequently, DNA-binding activity are temporally regulated, dramatically decreasing upon differentiation of MC3T3-E1 mouse osteoblasts. Corresponding with these results, the constitutive expression of C/EBPbeta LAP in MC3T3-E1 osteoblasts increased proliferation and suppressed osteogenic differentiation. Thus, C/EBPbeta LAP not only appears to participate in the regulation of genes associated with mature bone physiology, but is also a critical regulator of osteoblast growth and differentiation.
authors.affiliation: Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Vidhya V
authors.initials: VV
authors.lastname: Iyer
authors.forename: Tejas B
authors.initials: TB
authors.lastname: Kadakia
authors.forename: Laura R
authors.initials: LR
authors.lastname: McCabe
authors.forename: Richard C
authors.initials: RC
authors.lastname: Schwartz
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000831:Animals, Newborn; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000235:genetics / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D015675:Osteocalcin / Q000235:genetics
chemical_list: D022782:CCAAT-Enhancer-Binding Protein-beta; D004268:DNA-Binding Proteins; D015850:Interleukin-6; D015675:Osteocalcin; D007334:Insulin-Like Growth Factor I