title: Distribution of CD8 and CD20 lymphocytes in chronic periapical inflammatory lesions.
abstract: The objective of this study was to investigate the distribution of CD8+ and CD20+ lymphocytes in chronic periapical inflammatory lesions. A total of 90 periapical inflammatory lesions (chronic abscesses, abscessed cysts, and inflammatory cysts) were evaluated. The biotin-streptavidin immunohistochemical technique was used to identify cytotoxic/suppressor T-lymphocytes (CD8) and B-lymphocytes (CD20). Age ranged from 10 to 67 years. Patients between 26 and 45 years old (54.4%), females (52.2%), and white patients (74.4%) were more frequently affected. CD8+ cell distribution was as follows: 1) fibrous capsule: diffuse in 58.8% of chronic abscesses and absent in 64.1% of abscessed cysts and in 70.6% of inflammatory cysts; 2) infiltration zone: diffuse in 100% of abscessed cysts and in 82.4% of inflammatory cysts; 3) sub-epithelial zone: absent in 53.0% of inflammatory cysts and diffuse in 56.4% of abscessed cysts; 4) suppurative zone: diffuse in 100% of chronic abscesses and in 97.5% of abscessed cysts. CD20+ cell distribution was as follows: 1) fibrous capsule: absent in 100% of inflammatory cysts, in 94.8% of abscessed cysts, and in 88.3% of chronic abscesses; 2) infiltration zone: diffuse in 100% of abscessed cysts and in 53% of inflammatory cysts; 3) sub-epithelial zone: absent in 58.8% of inflammatory cysts and focal in 46.2% of abscessed cysts; 4) suppurative zone: diffuse in 100% of abscessed cysts and in 100% of chronic abscesses. The distribution of the lymphocytic infiltrate in the lesions was usually diffuse for both types of lymphocytes.
authors.affiliation: Department of Oral Pathology, School of Dentistry, UNIVALI, Itajaí, SC, Brazil.
authors.forename: Christine Kalvelage
authors.initials: CK
authors.lastname: Philippi
authors.forename: Pantelis Varvaki
authors.initials: PV
authors.lastname: Rados
authors.forename: Manoel
authors.initials: M
authors.lastname: Sant'ana Filho
authors.forename: João Jorge Diniz
authors.initials: JJ
authors.lastname: Barbachan
authors.forename: Onofre Francisco de
authors.initials: OF
authors.lastname: Quadros
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D018951:Antigens, CD20 / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology*; D016827:CD8 Antigens / Q000032:analysis; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000473:pathology*; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D002648:Child; D002908:Chronic Disease; D003238:Connective Tissue / Q000276:immunology / Q000473:pathology; D004848:Epithelium / Q000276:immunology / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010482:Periapical Abscess / Q000276:immunology / Q000473:pathology; D010485:Periapical Periodontitis / Q000276:immunology* / Q000473:pathology; D011842:Radicular Cyst / Q000276:immunology / Q000473:pathology; D012737:Sex Factors; D013492:Suppuration; D013602:T-Lymphocytes, Cytotoxic / Q000473:pathology; D044465:White People; D055815:Young Adult
chemical_list: D018951:Antigens, CD20; D016827:CD8 Antigens