title: Antimicrobial activity in methanolic extracts of several plant species from northern Argentina.
abstract: Thirty-nine native plant species were collected from the provinces of Chaco and Formosa, in northern Argentina, and were screened for antimicrobial activity. The plants were dried and extracted thoroughly with methanol. The dry extracts, dissolved in dimethylsulfoxide, were tested for inhibition of microbial growth via microplate assay with an oxidation-reduction dye. The test organisms were: Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus and Enterococcus faecium. Inhibition of respiratory activities in some of these microbial species was produced by the extracts of Astronium balansae, Geoffroea decorticans, Peltophorum dubium, Geoffroea spinosa, Lantana balansae, Prosopis kuntzei, Prosopis ruscifolia and Bulnesia sarmientoi, with minimal inhibitory concentrations (MIC) ranging from 0.08 to 0.5 mg dry matter/ml. Further in vitro experiments measuring the growth of S. aureus in liquid culture confirmed that all of the above extracts at 2 x MIC were able to inhibit bacterial growth effectively, and that some of them (A. balansae, G. decorticans, P. dubium, G. spinosa, P. kuntzei and B. sarmientoi) were able to reduce the initial number of viable counts by at least one order of magnitude in 10 hours, indicating that these extracts should be investigated further for the possible presence of bactericidal components.
authors.affiliation: Instituto de Patobiología, Centro Nacional de Investigaciones Agropecuarias, INTA-Castelar, Pcia. de Buenos Aires, Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Salvat
authors.forename: L
authors.initials: L
authors.lastname: Antonacci
authors.forename: R H
authors.initials: RH
authors.lastname: Fortunato
authors.forename: E Y
authors.initials: EY
authors.lastname: Suarez
authors.forename: H M
authors.initials: HM
authors.lastname: Godoy
mesh_terms: D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D001118:Argentina; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D006801:Humans; D008519:Medicine, Traditional; D008826:Microbial Sensitivity Tests; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D010946:Plants, Medicinal
chemical_list: D000890:Anti-Infective Agents; D010936:Plant Extracts