title: Immunolocalization of carbonic anhydrase and phosphoenolpyruvate carboxylase in developing seeds of Medicago sativa.
abstract: To investigate the role of carbonic anhydrase (CA; EC 4.2.1.1) and phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) during Medicago sativa seed development, the distribution of both proteins was examined using an immunohistological approach. Both enzymes are co-localized in most ovular and embryonic tissues. In early stages of seed development, both proteins were abundant in embryo and integuments, while at subsequent stages both proteins are accumulated in endosperm, nucellus and integuments. At late stages of seed development when both endosperm and nucellus are degraded, significant accumulation of both proteins was observed in the embryo proper. Chlorophyll was found to accumulate in embryos after the heart stage and reached a maximum at mature stage. It is suggested that CA and PEPC play a role in respiratory carbon dioxide refixation while generating malate to support amino acid and/or fatty acids biosynthesis.
authors.affiliation: Laboratory of Plant Physiology and Morphology, Iera Odos 75, 11855, Athens, Greece. gaivalakis@aua.gr
authors.forename: Georgios
authors.initials: G
authors.lastname: Aivalakis
authors.forename: Maria
authors.initials: M
authors.lastname: Dimou
authors.forename: Emmanouil
authors.initials: E
authors.lastname: Flemetakis
authors.forename: Fotini
authors.initials: F
authors.lastname: Plati
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Katinakis
authors.forename: J B
authors.initials: JB
authors.lastname: Drossopoulos
mesh_terms: D002256:Carbonic Anhydrases / Q000032:analysis*; D007150:Immunohistochemistry; D000455:Medicago sativa / Q000196:embryology* / Q000201:enzymology*; D010730:Phosphoenolpyruvate Carboxylase / Q000032:analysis*; D012639:Seeds / Q000201:enzymology* / Q000254:growth & development
chemical_list: D010730:Phosphoenolpyruvate Carboxylase; D002256:Carbonic Anhydrases