title: Estrogen receptor beta-mediated inhibition of male germ cell line development in mice by endogenous estrogens during perinatal life.
abstract: Epidemiological, clinical, and experimental studies have suggested that excessive exposure to estrogens during fetal/neonatal life can lead to reproductive disorders and sperm abnormalities in adulthood. However, it is unknown whether endogenous concentrations of estrogens affect the establishment of the male fetal germ cell lineage. We addressed this question by studying the testicular development of mice in which the estrogen receptor (ER) beta or the ERalpha gene was inactivated. The homozygous inactivation of ERbeta (ERbeta-/-) increased the number of gonocytes by 50% in 2- and 6-d-old neonates. The numbers of Sertoli and Leydig cells and the level of testicular testosterone production were unaffected, suggesting that estrogens act directly on the gonocytes. The increase in the number of gonocytes did not occur during fetal life but instead occurred just after birth, when gonocytes resumed mitosis and apoptosis. It seems to result from a decrease in the apoptosis rate evaluated by the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling method and cleaved caspase-3 immunohistochemical detection. Last, mice heterozygous for the ERbeta gene inactivation behaved similarly to their ERbeta-/- littermates in terms of the number of gonocytes, apoptosis, and mitosis, suggesting that these cells are highly sensitive to the binding of estrogens to ERbeta. ERalpha inactivation had no effect on the number of neonatal gonocytes and Sertoli cells. In conclusion, this study provides the first demonstration that endogenous estrogens can physiologically inhibit germ cell growth in the male. This finding may have important implications concerning the potential action of environmental estrogens.
authors.affiliation: Unité de Gamétogenèse et Génotoxicité, Institut National de la Sante et de la Recherche Medicale Unite 566, Commissariat a l'Energie Atomique, Universite Paris 7-Denis Diderot, 92265 Fontenay-aux-Roses, France.
authors.forename: Géraldine
authors.initials: G
authors.lastname: Delbès
authors.forename: Christine
authors.initials: C
authors.lastname: Levacher
authors.forename: Catherine
authors.initials: C
authors.lastname: Pairault
authors.forename: Chrystèle
authors.initials: C
authors.lastname: Racine
authors.forename: Clotilde
authors.initials: C
authors.lastname: Duquenne
authors.forename: Andrée
authors.initials: A
authors.lastname: Krust
authors.forename: René
authors.initials: R
authors.lastname: Habert
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D001835:Body Weight; D002455:Cell Division; D002460:Cell Line; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism; D007985:Leydig Cells / Q000166:cytology / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D011247:Pregnancy; D011960:Receptors, Estrogen / Q000235:genetics* / Q000378:metabolism*; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism; D013737:Testis / Q000166:cytology* / Q000196:embryology* / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; D011960:Receptors, Estrogen; D013739:Testosterone