title: [Detection of 85B mRNA in Mycobacterium tuberculosis cultures using the quantitative QRT-PCR (TaqMan) method].
abstract: The paper presents the in-house method of quantitative analysis of 85 B mRNA in Mycobacterium tuberculosis cultures coming from seeded biological material taken from tuberculosis patients. After the proper culture time, the total RNA was isolated. Than, a one-step QRT-PCR was performed. High specificity and sensitivity of the method was confirmed e.g. by participation in the Mycobacterium tuberculosis QC Proficiency Panel Programme (European Quality Control for Molecular Diagnostics, Glasgow, Scotland, UK).
authors.forename: Wanda
authors.initials: W
authors.lastname: Maksymowicz-Mazur
authors.forename: Joanna B
authors.initials: JB
authors.lastname: Pendzich
authors.forename: Urszula
authors.initials: U
authors.lastname: Mazurek
authors.forename: Adam
authors.initials: A
authors.lastname: Wilczok
authors.forename: Szymon
authors.initials: S
authors.lastname: Dworniczak
authors.forename: Jerzy
authors.initials: J
authors.lastname: Kozielski
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Oklek
mesh_terms: D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D012329:RNA, Bacterial / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D012329:RNA, Bacterial; D012333:RNA, Messenger