title: Factors affecting developmental competence of equine oocytes after intracytoplasmic sperm injection.
abstract: This study was conducted to evaluate the effect of initial cumulus morphology (expanded or compact) and duration of in vitro maturation (24, 30 or 42 h) on the developmental competence of equine oocytes after intracytoplasmic sperm injection (ICSI). The effect of manipulation temperature (room temperature vs 37 degrees C) at the time of ICSI and concentration of glucose (0.55 vs 5.5 mM) during embryo culture was also investigated. The nuclear maturation rates of expanded (Ex) oocytes were significantly (P < 0.001) higher than those of compact (Cp) oocytes at all maturation times (61-72 vs 23-25% respectively). Forty-eight hours after ICSI of mature Ex oocytes, the rate of cleavage with normal nuclei was significantly (P < 0.05) higher for oocytes matured for 24 h than for those matured for 30 or 42 h (73 vs 57-59% respectively). For Cp oocytes, the morphologic cleavage rates for oocytes matured for 30 h were significantly higher (P < 0.05) than for those matured for 24 or 42 h (86 vs 55-61% respectively). The overall proportion of embryos having more than four normal nuclei at 48 h culture was significantly higher (P < 0.05) for Cp than for Ex oocytes. Manipulation temperature did not affect development of embryos from Ex or Cp oocytes at 96 h after ICSI. Culture in high-glucose medium significantly increased morphologic cleavage of Cp, but not Ex, oocytes (P < 0.05). Embryos from Cp oocytes had a significantly higher average nucleus number after 96-h culture than did embryos from Ex oocytes. These data indicate that developmental competence differs between Ex and Cp equine oocytes, and is differentially affected by the duration of maturation and by composition of embryo culture media.
authors.affiliation: Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843-4466, USA.
authors.forename: Y H
authors.initials: YH
authors.lastname: Choi
authors.forename: L B
authors.initials: LB
authors.lastname: Love
authors.forename: D D
authors.initials: DD
authors.lastname: Varner
authors.forename: K
authors.initials: K
authors.lastname: Hinrichs
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002970:Cleavage Stage, Ovum / Q000187:drug effects*; D003470:Culture Media; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005260:Female; D005947:Glucose / Q000494:pharmacology; D006736:Horses; D008297:Male; D009865:Oocytes / Q000187:drug effects; D020554:Sperm Injections, Intracytoplasmic
chemical_list: D003470:Culture Media; D005947:Glucose