[Pathogenesis of rat mesangial proliferative glomerulonephritis induced by anti-Thy1 antibody].
OBJECTIVE
To probe into the pathogenesis of rat mesangial proliferative glomerulonephritis (MsPGN) induced by anti-Thy1 antibody.


METHODS
Anti-Thy1 serum was produced, and then intravenously injected into Wistar rats for establishing an experimental model of MsPGN. The control group received intravenous injection of normal saline. Urinary volume and urinary protein were examined every other day. The IL-1, IL-6 and TNF contents of serum were detected by radioimmunoassay. Pathologic morphology of renal section was observed with micrscope and BI2000 Image Analysis System. The rats of model group were killed on the 1st, 3rd, 5th and 7th days.


RESULTS
No significant difference was seen between the model group and control group in regard to the volume of urine and in-take water (P > 0.05). The levels of urinary protein, IL-1, IL-6 and TNF in model group were significantly higher than those in control group at all time points (P < 0.001-0.005). Glomerular mesangium cells and matrix in the model group were obviously proliferative, compared with those in control group.


CONCLUSIONS
It is suggested that cytokine plays an important role in the onset of MsPGN.
Zhang
Ke-fei
KF
Department of Nephrology, First Affiliated Hospital of Zunyi Medical College, Zunyi 563003, China.
Zhang
Liang
L
Wu
Xiong-fei
XF
Zhang
Xiang-gui
XG
Yu
Hong
H
Zhao
Shi-qi
SQ
D000818:Animals; D000906:Antibodies; D005920:Glomerular Mesangium / Q000378:metabolism; D015432:Glomerulonephritis, Membranoproliferative / Q000139:chemically induced / Q000209:etiology* / Q000378:metabolism; D007375:Interleukin-1 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018800:Thy-1 Antigens / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000906:Antibodies; D007375:Interleukin-1; D015850:Interleukin-6; D018800:Thy-1 Antigens; D014409:Tumor Necrosis Factor-alpha