[Pilot study of neonatal rat cardiac myocytes cultured for three-dimensional modeling in simulated microgravity].
OBJECTIVE
To study three-dimensional culturing methods of neonatal rat cardiac myocytes in simulated microgravity.


METHODS
Neonatal rat primary cardiac myocytes were separated and seeded into polylactic acid scaffolds, stirred in spinner flasks for 24 hours, and then moved into rotary cell culture system for three-dimensional culture. The growth of cardiac myocytes was observed under inverted phase contrast microscope, scanning electron microscope and transmission electron microscope, and metabolic assay was assessed by MTT assay.


RESULTS
Cardiac myocytes with sustained metabolic activity attached to the polylactic acid scaffolds, extended and confluenced. Pulsations of PLA-cardiac myocytes was found in some areas.


CONCLUSIONS
The rotary cell culture system is suitable to develop neonatal rat cardiac myocytes culturing for three-dimensional modeling.
Yang
Fen
F
Institute of Space Medico-Engineering, Beijing, P. R. China 100094.
Li
Ying-hui
YH
Nie
Jie-lin
JL
D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002470:Cell Survival; D002478:Cells, Cultured; D019344:Lactic Acid; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000648:ultrastructure; D011091:Polyesters; D011108:Polymers; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering; D018474:Weightlessness Simulation
D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)