The inhibition of cell spreading on a cellulose substrate (cuprophan) induces an apoptotic process via a mitochondria-dependent pathway.
Cell shape was found to be a strong indicator of whether individual cells grow or die, and may play an important role in controlling apoptosis as well as cell growth. We compared here the behaviour of rounded Swiss 3T3 cells aggregated on a cellulose cuprophan membrane to those cultured on dish polystyrene. We demonstrated that cells aggregated on cellulose substrates for up to 48 h underwent programmed cell death that was associated with phosphatidylserine flipping and caspase 9 and caspase 3 activation, suggesting a mitochondria-dependent apoptotic process. In addition, we found that this phenomenon cannot be entirely explained by disengagement of alpha 5 beta 1 integrin ligation.
Gekas
Jean
J
Domaine Biomatériaux-Biocompatibilité, UMR CNRS 6600, Université de Technologie de Compiègne, P.O. Box 20529, F-60205 Compiègne Cedex, France.
Hindié
Mathilde
M
Faucheux
Nathalie
N
Lanvin
Olivia
O
Mazière
Cécile
C
Fuentès
Vincent
V
Gouilleux-Gruart
Valérie
V
David
Bertrand
B
Mazière
Jean-Claude
JC
Lassoued
Kaïss
K
Nagel
Marie-Danielle
MD
D000818:Animals; D017209:Apoptosis; D001672:Biocompatible Materials; D020169:Caspases / Q000378:metabolism; D002448:Cell Adhesion; D002449:Cell Aggregation; D048429:Cell Size; D002482:Cellulose / Q000031:analogs & derivatives* / Q000378:metabolism*; D004789:Enzyme Activation; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D011137:Polystyrenes / Q000378:metabolism; D015398:Signal Transduction; D013379:Substrate Specificity; D041701:Swiss 3T3 Cells
D001672:Biocompatible Materials; D011137:Polystyrenes; D002482:Cellulose; C009865:cuprammonium cellulose; D020169:Caspases