Production of fumaric acid using rice bran and subsequent conversion to succinic acid through a two-step process.
The fungal production of fumaric acid using rice bran and subsequent bacterial conversion of succinic acid using fungal culture broth were investigated. Since the rice bran contains abundant proteins, amino acids, vitamins, and minerals, it is suitable material that fungi use as a nitrogen source. The effective concentration of rice bran to produce fumaric acid was 5 g/L. A large amount of rice bran caused excessive fungal growth rather than enhance fumaric acid production. In addition, we could produce fumaric acid without the addition of zinc and iron. Fungal culture broth containing approx 25 g/L of fumaric acid was directly employed for succinic acid conversion. The amount of glycerol and yeast extract required for succinic acid conversion was reduced to 70 and 30%, respectively, compared with the amounts cited in previous studies.
Moon
Se-Kwon
SK
School of Biological Sciences and Technology, Institute of Bioindustrial Technology, Chonnam National University, Gwangju 500-757, Korea.
Wee
Young-Jung
YJ
Yun
Jong-Sun
JS
Ryu
Hwa-Won
HW
D001671:Biochemistry / Q000379:methods*; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D002455:Cell Division; D004305:Dose-Response Relationship, Drug; D013293:Enterococcus faecalis / Q000378:metabolism; D005650:Fumarates / Q000737:chemistry* / Q000378:metabolism*; D005990:Glycerol / Q000737:chemistry; D012275:Oryza / Q000737:chemistry*; D012233:Rhizopus / Q000378:metabolism; D019802:Succinic Acid / Q000737:chemistry* / Q000378:metabolism; D013997:Time Factors
D005650:Fumarates; D002244:Carbon; C032005:fumaric acid; D019802:Succinic Acid; D005990:Glycerol