Serotyping, stx2 subtyping, and characterization of the locus of enterocyte effacement island of shiga toxin-producing Escherichia coli and E. coli O157:H7 strains isolated from the environment in France.
Twenty-seven Shiga toxin-producing Escherichia coli (STEC) strains were isolated from 207 stx-positive French environmental samples. Ten of these strains were positive for stx(1), and 24 were positive for stx(2) (10 were positive for stx(2vh-a) or stx(2vh-b), 19 were positive for stx(2d), and 15 were positive for stx(2e)). One strain belonged to serotype O157:H7, and the others belonged to serogroups O2, O8, O11, O26, O76, O103, O113, O121, O141, O166, and O174. The environment is a reservoir in which new clones of STEC that are pathogenic for humans can emerge.
Vernozy-Rozand
C
C
Unité de Microbiologie Alimentaire et Prévisionnelle, Ecole Nationale Vétérinaire de Lyon, Marcy l'Etoile, France. c.vernozy@vet-lyon.fr
Montet
M P
MP
Bertin
Y
Y
Trably
F
F
Girardeau
J P
JP
Martin
C
C
Livrelli
V
V
Beutin
L
L
D015373:Bacterial Typing Techniques; D004783:Environmental Microbiology; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D019453:Escherichia coli O157 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D029968:Escherichia coli Proteins / Q000235:genetics*; D005602:France; D005798:Genes, Bacterial; D005838:Genotype; D010750:Phosphoproteins / Q000235:genetics*; D012703:Serotyping; D022621:Shiga Toxin / Q000096:biosynthesis; D022641:Shiga Toxin 2 / Q000096:biosynthesis / Q000235:genetics*
D029968:Escherichia coli Proteins; C457340:LEE protein, E coli; D010750:Phosphoproteins; D022641:Shiga Toxin 2; D022621:Shiga Toxin