BAC resources for the rat genome project.
Two 11-fold redundant bacterial artificial chromosome (BAC) libraries (RPCI-32 and CHORI-230) have been constructed to support the rat genome project. The first library was constructed using a male Brown Norway (BN/SsNHsd) rat as a DNA source long before plans for rat genome sequencing had been launched. The second library was prepared from a highly inbred female (BN/SsNHsd/MCW) rat in support of the rat genome sequencing project. The use of an inbred rat strain is essential to avoid problems with genome assembly resulting from the difficulty of distinguishing haplotype variation from variation among duplicons. We have demonstrated the suitability of the library by using a detailed quality assessment of large insert sizes, narrow size distribution, consistent redundancy for many markers, and long-range continuity of BAC contig maps. The widespread use of the two libraries as an integral part of the rat genome project has led to the database annotations for many clones, providing rat researchers with a rich resource of BAC clones that can be screened in silico for genes of interest.
Osoegawa
Kazutoyo
K
Children's Hospital and Research Center at Oakland, Oakland, California 94609, USA.
Zhu
Baoli
B
Shu
Chung Li
CL
Ren
Teresa
T
Cao
Qing
Q
Vessere
Gery M
GM
Lutz
Michelle M
MM
Jensen-Seaman
Michael I
MI
Zhao
Shaying
S
de Jong
Pieter J
PJ
D000818:Animals; D001947:Breeding; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D020451:Contig Mapping / Q000379:methods; D003433:Crosses, Genetic; D005069:Evaluation Studies as Topic; D005260:Female; D015723:Gene Library; D005822:Genetic Vectors / Q000235:genetics; D016678:Genome; D008297:Male; D020161:Physical Chromosome Mapping / Q000379:methods; D011786:Quality Control; D051381:Rats; D011914:Rats, Inbred BN; D017207:Rats, Sprague-Dawley; D017422:Sequence Analysis, DNA / Q000379:methods / Q000592:standards