title: Determination of vigabatrin in human plasma and urine by high-performance liquid chromatography with UV-Vis detection.
abstract: A simple and reliable high-performance liquid chromatographic (HPLC) method with UV-Vis detection has been developed and validated for the determination of vigabatrin (VG) in human plasma and urine. The samples were pre-column derivatizated with 1,2-naphthoquinone-4-sulphonic acid sodium salt (NQS). A good chromatographic separation was achieved on a C18 column with a mobile phase consisting of acetonitrile and 10 mM orthophosphoric acid (pH 2.5) gradient elution. Tranexamic acid was used as an internal standard (I.S.). The method was linear over the concentration range of 0.8-30.0 microg/ml for both samples. The method is precise (relative standard deviation (R.S.D.) <9.13%) and accurate (relative mean error (RME) <-8.75%); analytical recoveries were 81.07% for plasma and 83.05% for urine. The assay was applied to pharmacokinetic study in a healthy volunteer after a single oral administration of 1 g of vigabatrin.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Istanbul University, Beyazit, 34116 Istanbul, Turkey. sevilmuge@yahoo.com
authors.forename: Sevil Müge
authors.initials: SM
authors.lastname: Cetin
authors.forename: Sedef
authors.initials: S
authors.lastname: Atmaca
mesh_terms: D000927:Anticonvulsants / Q000032:analysis* / Q000493:pharmacokinetics; D000933:Antifibrinolytic Agents / Q000032:analysis; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D015203:Reproducibility of Results; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D014148:Tranexamic Acid / Q000032:analysis; D020888:Vigabatrin / Q000032:analysis* / Q000493:pharmacokinetics
chemical_list: D000927:Anticonvulsants; D000933:Antifibrinolytic Agents; D007202:Indicators and Reagents; D012996:Solutions; D014148:Tranexamic Acid; D020888:Vigabatrin