PCR hot-start using duplex primers.
A new technique of PCR hot-start using duplex primers has been developed which can decrease the undesirable products arising throughout PCR amplification thereby giving better results than a manual hot-start method.
Kong
Deming
D
State Key Laboratory, of Functional Polymer Materials for Adsorption and Separation, Chemical School, Nankai University, Tianjin 300071, P.R. China. kongdem@eyou.com
Shen
Hanxi
H
Huang
Yanping
Y
Mi
Huaifeng
H
D001709:Biotechnology / Q000379:methods*; D004247:DNA / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D006515:Hepatitis B virus / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods*
D017931:DNA Primers; D004247:DNA