Induction of baroresistance by hydrogen peroxide, ethanol and cold-shock in Saccharomyces cerevisiae.
The acquisition of tolerance to high hydrostatic pressure of 220 MPa (HHP) in response to a 0.4 mM hydrogen peroxide, 6% ethanol and cold-shock (10 degrees C) pretreatment for different lengths of times was studied in the yeast Saccharomyces cerevisiae. The protection conferred by these different treatments was similar ( approximately 3 log cycles) and time-dependent. Analysis of the induction of the most pressure up-regulated genes under these conditions was investigated by RT-PCR. Our results revealed that the cell stress response to HHP shares common features with hydrogen peroxide and ethanol stresses, but differs in some way to cold-shock.
Palhano
Fernando L
FL
Departamento de Ciências Fisiológicas, Universidade Federal do Espírito Santo, Av. Marechal Campos, 1468, 29040-090, Vitória, ES, Brazil.
Orlando
Marcos T D
MT
Fernandes
Patricia M B
PM
D000199:Actins / Q000235:genetics / Q000502:physiology; D000222:Adaptation, Physiological; D003080:Cold Temperature; D015169:Colony Count, Microbial; D000431:Ethanol; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D006360:Heat-Shock Proteins / Q000235:genetics / Q000502:physiology; D006861:Hydrogen Peroxide; D006874:Hydrostatic Pressure; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology; D013997:Time Factors
C465210:Act1 protein, S cerevisiae; D000199:Actins; D006360:Heat-Shock Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol; D006861:Hydrogen Peroxide