title: Effect of temperature, moisture, and carbon supplementation on lipase production by solid-state fermentation of soy cake by Penicillium simplicissimum.
abstract: The production of lipases by Penicillium simplicissimum using solid-state fermentation and soy cake as substrate was investigated. The effects of temperature, cake moisture, and carbon supplementation on lipase production were studied using a two-level experimental plan. Moisture, pH, and lipase activity were followed during fermentation. Statistical analysis of the results was performed to evaluate the effect of the studied variables on the maximum lipase activity. Incubation temperature was the variable that most affected enzyme activity, showing a negative effect. Moisture and carbon supplementation presented a positive effect on activity. It was possible to obtain lipase activity as high as 21 U/g of dry cake in the studied range of process variables.
authors.affiliation: Department of Food Engineering, URI-Campus de Erechim, Av. Sete de Setembro 1621, Erechim, 99700-000, RS, Brazil. diluccio@uricer.edu.br
authors.forename: Marco
authors.initials: M
authors.lastname: Di Luccio
authors.forename: Fernando
authors.initials: F
authors.lastname: Capra
authors.forename: Najara P
authors.initials: NP
authors.lastname: Ribeiro
authors.forename: Gean D L P
authors.initials: GD
authors.lastname: Vargas
authors.forename: Denise M G
authors.initials: DM
authors.lastname: Freire
authors.forename: Débora
authors.initials: D
authors.lastname: de Oliveira
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000096:biosynthesis / Q000737:chemistry*; D000069463:Olive Oil; D010407:Penicillium / Q000378:metabolism*; D010938:Plant Oils; D013025:Glycine max / Q000382:microbiology*; D013696:Temperature; D013997:Time Factors
chemical_list: D003470:Culture Media; D000069463:Olive Oil; D010938:Plant Oils; D002244:Carbon; D008049:Lipase