title: Characterization of the first VanB vancomycin-resistant Enterococcus faecium isolated in a Spanish hospital.
abstract: We report the detection and characterization of the first vancomycin-resistant VanB-type Enterococcus faecium to be isolated in a Spanish hospital. Sequence analysis of the vanB gene showed that this isolate belonged to subtype vanB2. Moreover, PCR amplification analysis indicated that the vanB gene cluster was linked to a Tn5382-like transposon.
authors.affiliation: Molecular Biology Laboratory, Research Unit, Nuestra Señora de Candelaria University Hospital, Santa Cruz de Tenerife, Spain.
authors.forename: Fabián
authors.initials: F
authors.lastname: Lorenzo-Díaz
authors.forename: Teresa
authors.initials: T
authors.lastname: Delgado
authors.forename: José A
authors.initials: JA
authors.lastname: Reyes-Darias
authors.forename: Carlos
authors.initials: C
authors.lastname: Flores
authors.forename: Sebastián
authors.initials: S
authors.lastname: Méndez-Alvarez
authors.forename: Jesús
authors.initials: J
authors.lastname: Villar
authors.forename: Antonio
authors.initials: A
authors.lastname: Sierra
authors.forename: Félix
authors.initials: F
authors.lastname: Claverie-Martín
mesh_terms: D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics / Q000502:physiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D024881:Drug Resistance, Bacterial; D016984:Enterococcus faecium / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D005798:Genes, Bacterial / Q000235:genetics / Q000502:physiology; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006761:Hospitals; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D003668:Pressure Ulcer / Q000382:microbiology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013030:Spain; D014640:Vancomycin / Q000494:pharmacology*; D020713:Vancomycin Resistance
chemical_list: D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D014640:Vancomycin