title: [Effect of bushen ningxin decoction on proliferation and apoptosis of murine osteoblast].
abstract: OBJECTIVE
  To investigate the effect of Bushen Ningxin decoction (BSNXD) on postmenopausal osteoporosis.
  
  
  METHODS
  Postmenopausal osteoporosis model of female BALB/c mice were founded and afforded with BSNXD. The experiment was ceased, and the skeleton and blood of mice in each group were collected following 12 weeks of the treatment. Change of osteoblastic organelle in right femur was observed by transmission electron microscope (TEM). Osteoblasts from calvaria of different groups were isolated and cultured by the tissue fragment migrating growth method and the proliferation ratio of osteoblasts in the second passage was examined with thiazolyl blue reagents when cultured for 1 day and 5 days, respectively.
  
  
  RESULTS
  TEM showed that the osteoblasts of the animal treated by BSNXD had more cell organelles and developed Golgi complex, whose ability of proliferation(P < 0.05) and anti-apoptosis is stronger than that of OVX group.
  
  
  CONCLUSIONS
  BSNXD can accelerate the proliferation and inhibit the apoptosis of osteoblasts, the effective concentration of pharmacological serum varies from 10% to 20%.
authors.affiliation: Laboratory of Reproductive Immunology, Institute of Obstetrics & Gynecology, Fudan University, Shanghai 200011.
authors.forename: Yu-dong
authors.initials: YD
authors.lastname: Wang
authors.forename: Da-jin
authors.initials: DJ
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D010006:Osteoblasts / Q000473:pathology*; D010024:Osteoporosis / Q000209:etiology / Q000473:pathology*; D010052:Ovariectomy; D011897:Random Allocation
chemical_list: D004365:Drugs, Chinese Herbal