Analysis of microbial diversity in oligotrophic microbial fuel cells using 16S rDNA sequences.
Molecular ecological techniques were applied to analyze the bacterial diversity of two oligotrophic microbial fuel cells (MFCs) enriched using river water or artificial wastewater (AWW) as fuel. Denaturing gradient gel electrophoresis (DGGE) of the PCR amplified 16S rDNA showed that different microbial communities were present in the two MFCs and these were different from the river sediment used to initiate the enrichment. Nearly complete 16S rDNA was amplified and sequenced. Over 80% of the clones were Proteobacteria. Betaproteobacteria were the dominant clones (46.2%) in MFCs fed with river water, and about 64.4% of the clones in MFCs fed with AWW were Alphaproteobacteria. Actinobacteria were found only in the MFC fed with AWW, and Deltaproteobacteria, Acidobacteria, Chloroflexi and Verrucomicrobia in the MFC fed with river water. Many clones were related to uncultured bacteria, some with homology less than 95%, indicating that many novel bacteria were enriched in the oligotrophic MFCs.
Phung
Nguyet Thu
NT
Water Environment and Remediation Research Center, Korea Institute of Science and Technology, 39-1, Hawolgok dong, Sungbuk gu, Seoul 136-791, Republic of Korea.
Lee
Jiyoung
J
Kang
Kui Hyun
KH
Chang
In Seop
IS
Gadd
Geoffrey Michael
GM
Kim
Byung Hong
BH
D039903:Actinobacteria / Q000302:isolation & purification; D020561:Alphaproteobacteria / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D020562:Betaproteobacteria / Q000302:isolation & purification; D044822:Biodiversity; D001674:Bioelectric Energy Sources; D041862:Chloroflexi / Q000302:isolation & purification; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D020564:Deltaproteobacteria / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004734:Energy Metabolism; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014867:Water
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D014867:Water