Interlaboratory testing of porcine sera for antibodies to porcine circovirus type 2.
A panel of 20 porcine sera was distributed to 5 laboratories across Europe and Canada. Each center was requested to test the sera for the presence of porcine circovirus type 2 antibodies using the routine assays, indirect immunofluorescence assay (IFA) and indirect immunoperoxidase monolayer assay (IPMA), and to determine the titer of each serum. Results from all centers were then compiled and correlated. They demonstrate a wide variation in the titers obtained between laboratories. These differences were dependent on the assay used and the choice of fixative. In general, IPMA gave higher titers than did IFA, and paraformaldehyde gave higher titers than did acetone or ethyl alcohol. This report highlights the need for standardized procedures and biologicals for this virus.
McNair
Irene
I
Department of Agriculture and Rural Development, Veterinary Sciences Division, Stormont, Belfast BT4 3SD, Northern Ireland.
Marshall
Michelle
M
McNeilly
Francis
F
Botner
Anette
A
Ladekjaer-Mikkelsen
Anne-Sofie
AS
Vincent
Isabelle
I
Herrmann
Brigitte
B
Sanchez
Romeo
R
Rhodes
Carrie
C
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D018173:Circoviridae Infections / Q000097:blood / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000276:immunology*; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D007124:Immunoenzyme Techniques / Q000662:veterinary; D015203:Reproducibility of Results; D013048:Specimen Handling / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000276:immunology / Q000821:virology*
D000914:Antibodies, Viral