title: Neonatal capsaicin treatment affects rat thymocyte proliferation and cell death by modulating substance P and neurokinin-1 receptor expression.
abstract: Herein we provide evidence that substance P (SP) and its neurokinin-1 receptor (NK-1R) expressed on thymocytes counteract thymus depletion induced by neonatal capsaicin (CPS) treatment by affecting thymocyte proliferation and apoptotic death. SP administration reversed the CPS-mediated inhibitory effects on the total thymocyte number and subset distribution, namely CD4+ and CD4- CD8- cells, through its interaction with NK-1R as shown by concomitant NK-1R (SR140333) antagonist administration. SP-induced enhancement of thymus cellularity parallels its ability of inhibiting the thymocyte apoptotic program. Indeed, exogenously administered SP completely nullified CPS-induced apoptosis, and SR140333 abrogated the SP-mediated protective effect. SP administration also stimulated concanavalin A (Con A)-induced thymocyte proliferation of CPS-treated rats, completely reversing the CPS-induced inhibition. The SP-mediated stimulation of Con A-induced thymocyte proliferation was NK-1R dependent as shown by concomitant administration of SP and SR140333 to CPS-treated rats. Our results also demonstrate that CPS treatment induces a marked decrease of thymocyte PPT-A mRNA level and endogenous SP content as evaluated by quantitative RT-PCR, in situ hybridization and cytofluorimetric analysis. By contrast, NK-1R mRNA levels were increased in thymocytes from CPS-treated rats. Exogenous SP administration augmented PPT-A, SP and NK-1R thymocyte expression in CPS-treated rats, and this enhancement was antagonized by SR140333 administration. Overall, our results strongly suggest that the immunomodulatory effects of neonatal CPS treatment on rat thymocyte functions are dependent on vanilloid-mediated regulation of SP and NK-1R functional expression by neuronal and immune cells.
authors.affiliation: Department of Pharmacological Sciences and Experimental Medicine, University of Camerino, Camerino, Italy. giorgio.santoni@unicam.it
authors.forename: Giorgio
authors.initials: G
authors.lastname: Santoni
authors.forename: Consuelo
authors.initials: C
authors.lastname: Amantini
authors.forename: Roberta
authors.initials: R
authors.lastname: Lucciarini
authors.forename: Marina
authors.initials: M
authors.lastname: Perfumi
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Pompei
authors.forename: Mario
authors.initials: M
authors.lastname: Piccoli
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D001284:Atrophy / Q000139:chemically induced / Q000276:immunology / Q000503:physiopathology; D019898:Autocrine Communication / Q000187:drug effects / Q000276:immunology; D001665:Binding Sites / Q000187:drug effects / Q000276:immunology; D002211:Capsaicin / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects / Q000276:immunology; D002455:Cell Division / Q000187:drug effects / Q000276:immunology; D003208:Concanavalin A / Q000494:pharmacology; D006133:Growth Substances / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D008297:Male; D015213:Neuroimmunomodulation / Q000187:drug effects / Q000276:immunology; D064729:Neurokinin-1 Receptor Antagonists; D010880:Piperidines / Q000494:pharmacology; D011812:Quinuclidines / Q000494:pharmacology; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018040:Receptors, Neurokinin-1 / Q000819:agonists / Q000378:metabolism*; D013373:Substance P / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D013950:Thymus Gland / Q000187:drug effects* / Q000276:immunology / Q000503:physiopathology; D015854:Up-Regulation / Q000187:drug effects / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D006133:Growth Substances; D064729:Neurokinin-1 Receptor Antagonists; D010880:Piperidines; D011812:Quinuclidines; D012333:RNA, Messenger; D018040:Receptors, Neurokinin-1; D003208:Concanavalin A; C085215:SR 140333; D013373:Substance P; D002211:Capsaicin