A study for minisatellitic markers of Conidiobolus coronatus' pathogenicity to Galleria mellonella larvae.
A selected panel of 13 colonies of entomopathogenic fungus Conidiobolus coronatus representing 6 variants of pathogenicity to Galleria mellonella larvae (ranged from 100 to 10% of efficiency), derived from single spores, were tested for the presence of hypervariable loci in their genomes by hybridization with Jeffreys' human minisatellite probe 33.6. The probe produced informative fingerprints and revealed slight differences among colonies analyzed. Up to 20 variable bands per colony were recognized in the size range of 2-20 kb. The band sharing within groups with the same pathogenicity ranged from 0.966 to 0.800. The genetic distance between different variants ranged from 0.026 to 0.282. A few characteristic bands for high and low pathogenicity to the larvae were found.
Wieloch
Wioletta
W
Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818 Warsaw, Poland. wiolaw@twarda.pan.pl
Sacharczuk
Mariusz
M
Boguś
Mieczysława I
MI
Jaszczak
Kazimierz
K
D000818:Animals; D015825:Chromosomes, Fungal / Q000235:genetics*; D022801:Complementarity Determining Regions / Q000235:genetics*; D020092:Conidiobolus / Q000235:genetics* / Q000472:pathogenicity*; D016172:DNA Fingerprinting; D015342:DNA Probes; D005819:Genetic Markers / Q000235:genetics; D007814:Larva / Q000382:microbiology; D018598:Minisatellite Repeats / Q000235:genetics*; D009036:Moths / Q000382:microbiology*; D013045:Species Specificity
D022801:Complementarity Determining Regions; D015342:DNA Probes; D005819:Genetic Markers