Proteomic mapping provides powerful insights into functional myelin biology.
Myelin is a dynamic, functionally active membrane necessary for rapid action potential conduction, axon survival, and cytoarchitecture. The number of debilitating neurological disorders that occur when myelin is disrupted emphasizes its importance. Using high-resolution 2D gel electrophoresis, mass spectrometry, and immunoblotting, we have developed an extensive proteomic map of proteins present in myelin, identifying 98 proteins corresponding to at least 130 of the approximately 200 spots on the map. This proteomic map has been applied to analyses of the localization and function of selected proteins, providing a powerful tool to investigate the diverse functions of myelin.
Taylor
Christopher M
CM
Department of Neuroscience, MC 3401, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030-3401, USA.
Marta
Cecilia B
CB
Claycomb
Robert J
RJ
Han
David K
DK
Rasband
Matthew N
MN
Coetzee
Timothy
T
Pfeiffer
Steven E
SE
D000818:Animals; D001369:Axons / Q000502:physiology / Q000648:ultrastructure; D015180:Electrophoresis, Gel, Two-Dimensional; D004798:Enzymes / Q000302:isolation & purification; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D023421:Models, Animal; D009185:Myelin Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000302:isolation & purification; D010449:Peptide Mapping; D020543:Proteome; D011817:Rabbits; D012583:Schwann Cells / Q000201:enzymology / Q000502:physiology
D004798:Enzymes; D009185:Myelin Proteins; D009419:Nerve Tissue Proteins; D020543:Proteome