Fast automatic particle picking from cryo-electron micrographs using a locally normalized cross-correlation function: a case study.
Recent progress in single-particle reconstruction methods and cryo-EM techniques has led to the determination of macromolecular structures with unprecedented resolution. The number of particles that goes into the reconstruction is a key determinant in achieving high resolution. Interactive manual picking of particles from an electron micrograph is a very time-consuming, tedious, and inefficient process. We have implemented a fast automatic particle picking procedure in the SPIDER environment. The procedure makes use of template matching schemes and employs a recently developed locally normalized correlation algorithm based on Fourier techniques. As a test, we have used this procedure to pick 70S Escherichia coli ribosomes from a cryo-electron micrograph. Different search strategies including use of a circular mask and asymmetric masks for different orientations of the particle have been explored, and their relative efficiencies are discussed. The results indicate that the procedure can be optimally used to pick ribosomes in a fully automatic way within the limit of selecting less than 10% false positives while missing about 15% of true positives.
Rath
B K
BK
Wadsworth Center, Health Research, Inc., Empire State Plaza, Albany, NY 12201-0509, USA.
Frank
J
J
D000465:Algorithms; D020285:Cryoelectron Microscopy / Q000379:methods*; D001330:Electronic Data Processing / Q000379:methods; D004926:Escherichia coli / Q000737:chemistry; D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional; D046911:Macromolecular Substances; D015233:Models, Statistical; D010363:Pattern Recognition, Automated; D012270:Ribosomes / Q000737:chemistry / Q000648:ultrastructure; D012984:Software
D046911:Macromolecular Substances