Fibroin silk proteins from the nonmulberry silkworm Philosamia ricini are biochemically and immunochemically distinct from those of the mulberry silkworm Bombyx mori.
Silk proteins were isolated from the cocoons of the nonmulberry silkworm, Philosamia ricini. Three polypeptides of 97, 66, and 45 kDa were identified. The 66-kDa molecule represented sericin, whereas the 97-kDa and the 45-kDa polypeptides linked together through a disulfide bond constituted the fibroin protein. Antibodies raised against the 97-kDa P. ricini fibroin heavy chain reacted specifically with this molecule and did not recognize fibroin heavy chain from another nonmulberry silkworm, Antheraea assama or from the mulberry silkworm, Bombyx mori, suggesting the presence of P. ricini species-specific determinants in this heavy chain. Antibodies generated against fibroin light chain of P. ricini also showed similar reactivity pattern. Immunoblot analysis with proteins isolated from the silk glands of P. ricini at different stages of larval development showed that the expression of fibroin heavy chain was developmentally and spatially regulated. The protein was most abundant in the 5th instar larva, and could be detected in the middle and the posterior but not the anterior silk glands. The amino acid composition of the 97-kDa fibroin protein showed abundance of glutamic acid and did not contain (Gly-Ala)(n) motifs, a characteristic feature of B. mori fibroin heavy chain. Our study reveals significant differences between the nonmulberry silkworm P. ricini and the mulberry silkworm B. mori in the biochemical composition and immunochemical characteristics of fibroin heavy chain. These differences might be responsible for the differences seen in the quality of silk produced by these two silkworms.
Ahmad
Raies
R
Laboratory of Eukaryotic Gene Expression, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110 067, India.
Kamra
Anita
A
Hasnain
Seyed Ehtesham
SE
D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D012831:Bombyx / Q000737:chemistry* / Q000145:classification / Q000276:immunology; D005349:Fibroins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D005455:Fluorescent Antibody Technique; D019476:Insect Proteins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D007814:Larva / Q000737:chemistry / Q000276:immunology; D047011:Silk
D000596:Amino Acids; D019476:Insect Proteins; D047011:Silk; D005349:Fibroins