S-layer glycan-specific loci on the chromosome of Geobacillus stearothermophilus NRS 2004/3a and dTDP-L-rhamnose biosynthesis potential of G. stearothermophilus strains.
The approximately 16.5 kb surface layer (S-layer) glycan biosynthesis (slg) gene cluster of the Gram-positive thermophile Geobacillus stearothermophilus NRS 2004/3a has been sequenced. The cluster is located immediately downstream of the S-layer structural gene sgsE and consists of 13 ORFs that have been identified by database sequence comparisons. The cluster encodes dTDP-L-rhamnose biosynthesis (rml operon), required for building up the polyrhamnan S-layer glycan, as well as for assembly and export of the elongated glycan chain, and its transfer to the S-layer protein. This is the first report of a gene cluster likely to be involved in the glycosylation of an S-layer protein. There is evidence that this cluster is transcribed as a polycistronic unit, whereas sgsE is transcribed monocistronically. To get insights into the regulatory mechanisms underlying glycosylation of the S-layer protein, the influence of growth temperature on the S-layer was investigated in seven closely related G. stearothermophilus strains, of which only strain NRS 2004/3a possessed a glycosylated S-layer. Chromosomal DNA preparations of these strains were screened for the presence of the rml operon, because L-rhamnose is a frequent constituent of S-layer glycans. From rml-positive strains, flanking regions of the operon were sequenced. Comparison with the slg gene cluster of G. stearothermophilus NRS 2004/3a revealed sequence homologies between adjacent genes. The temperature inducibility of S-layer protein glycosylation was investigated in those strains by raising the growth temperature from 55 degrees C to 67 degrees C; no change of either the protein banding pattern or the glycan staining behaviour was observed on SDS-PAGE gels, although the sgsE transcript was several-fold more abundant at 67 degrees C. Cell-free extracts of the strains were capable of converting dTDP-D-glucose to dtdp-L-rhamnose. Taken together, the results indicate that the rml locus is highly conserved among G. stearothermophilus strains, and that in the investigated rml-containing strains, dTDP-L-rhamnose is actively synthesized in vitro. However, in contrast to previous reports for G. stearothermophilus wild-type strains, an increase in growth temperature did not switch an S-layer protein phenotype to an S-layer glycoprotein phenotype, via the de novo generation of a new S-layer gene sequence.
Novotny
René
R
Center for NanoBiotechnology, University of Applied Life Sciences and Natural Resources, A-1180 Wien, Austria.
Schäffer
Christina
C
Center for NanoBiotechnology, University of Applied Life Sciences and Natural Resources, A-1180 Wien, Austria.
Strauss
Joseph
J
Center of Applied Genetics, University of Applied Life Sciences and Natural Resources, A-1190 Wien, Austria.
Messner
Paul
P
Center for NanoBiotechnology, University of Applied Life Sciences and Natural Resources, A-1180 Wien, Austria.
D001406:Bacillaceae / Q000235:genetics* / Q000254:growth & development; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002876:Chromosomes, Bacterial; D015964:Gene Expression Regulation, Bacterial; D006031:Glycosylation; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009702:Nucleoside Diphosphate Sugars / Q000096:biosynthesis*; D011134:Polysaccharides / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013696:Temperature; D013942:Thymine Nucleotides / Q000096:biosynthesis*
D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; D009702:Nucleoside Diphosphate Sugars; D011134:Polysaccharides; C055764:S-layer proteins; D013942:Thymine Nucleotides; C073293:thymidine diphosphate rhamnose