title: Acidophilic granulocytes of the marine fish gilthead seabream (Sparus aurata L.) produce interleukin-1beta following infection with Vibrio anguillarum.
abstract: The fish immune response to Gram-negative bacteria is poorly understood. In this study, we use a monoclonal antibody (mAb) specific to acidophilic granulocytes from the marine fish gilthead seabream (Sparus aurata L.), together with an antiserum specific to interleukin-1beta (IL-1beta) from this species, in order to investigate whether these cells are involved in the immune response against the pathogenic bacterium Vibrio anguillarum and, in particular, in the production of the pro-inflammatory cytokine IL-1beta. We found that gilthead seabream head-kidney, peritoneal exudate and peripheral blood leukocytes accumulated proIL-1beta intracellularly when challenged in vitro with V. anguillarum, whereas only peritoneal exudate and blood leukocytes were able to accumulate proIL-1beta following infection. Importantly, the blood leukocytes from infected animals that accumulated proIL-1beta were shown to be the acidophilic granulocytes. A rapid mobilization of such cells from the head-kidney to the site of inflammation following infection with V. anguillarum was also observed.
authors.affiliation: Department of Cell Biology, Faculty of Biology, University of Murcia, Campus Universitario de Espinardo, 30100 Murcia, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Chaves-Pozo
authors.forename: Pablo
authors.initials: P
authors.lastname: Pelegrín
authors.forename: Jesús
authors.initials: J
authors.lastname: García-Castillo
authors.forename: Alfonsa
authors.initials: A
authors.lastname: García-Ayala
authors.forename: Victoriano
authors.initials: V
authors.lastname: Mulero
authors.forename: José
authors.initials: J
authors.lastname: Meseguer
mesh_terms: D000818:Animals; D005393:Fish Diseases / Q000276:immunology*; D006098:Granulocytes / Q000276:immunology*; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000096:biosynthesis*; D007668:Kidney / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D021541:Sea Bream / Q000276:immunology*; D014733:Vibrio / Q000378:metabolism*; D014735:Vibrio Infections / Q000276:immunology / Q000662:veterinary*
chemical_list: D007375:Interleukin-1