title: Genotyping and antifungal susceptibility profile of Dipodascus capitatus isolates causing disseminated infection in seven hematological patients of a tertiary hospital.
abstract: Seven cases of disseminated infection due to Dipodascus capitatus are reported. Infections occurred in a hematological unit of a tertiary hospital during a period of 5 years. Five cases were refractory to antifungal therapy. Antifungal susceptibility testing of seven isolates was performed, and strains were typed by PCR fingerprinting with the core sequence of phage M13 and by random amplification of polymorphic DNA with two primers, Ap12h and W-80A. A very short range of MICs of each antifungal agent was observed. The MICs of amphotericin B ranged between 0.50 and 2 microg/ml. Strains were susceptible in vitro to flucytosine and susceptible (dose-dependent) to fluconazole and itraconazole. Voriconazole exhibited an activity in vitro comparable to that of itraconazole. Typing techniques allowed seven additional isolates of D. capitatus neither geographically nor temporally related to be classified into two different genomic patterns. The genomic type of the seven strains from the hematological unit was identical regardless of typing technique utilized. It would indicate that the seven cases of disseminated infection could be related epidemiologically.
authors.affiliation: Departamento de Microbiología, Madrid, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Gadea
authors.forename: Manuel
authors.initials: M
authors.lastname: Cuenca-Estrella
authors.forename: Elena
authors.initials: E
authors.lastname: Prieto
authors.forename: Teresa M
authors.initials: TM
authors.lastname: Diaz-Guerra
authors.forename: Jose I
authors.initials: JI
authors.lastname: Garcia-Cia
authors.forename: Emilia
authors.initials: E
authors.lastname: Mellado
authors.forename: Jose F
authors.initials: JF
authors.lastname: Tomas
authors.forename: Juan L
authors.initials: JL
authors.lastname: Rodriguez-Tudela
mesh_terms: D000328:Adult; D000368:Aged; D000935:Antifungal Agents / Q000494:pharmacology; D002555:Cerebrospinal Fluid / Q000382:microbiology; D016172:DNA Fingerprinting / Q000379:methods; D005260:Female; D016469:Fungemia / Q000382:microbiology*; D005838:Genotype; D006761:Hospitals; D006801:Humans; D007938:Leukemia / Q000150:complications*; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D016533:Mycological Typing Techniques; D009181:Mycoses / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D004718:Saccharomycetales / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000935:Antifungal Agents