title: Chemosensitivity assessed by collagen gel droplet embedded culture drug sensitivity test, and MDR1, MRP1, and MRP2 mRNA expression in human colorectal adenocarcinomas.
abstract: OBJECTIVE
  To evaluate chemosensitivity and its correlation with expression levels of the multidrug resistant transporter (MDR1) and the multidrug resistance-associated proteins 1 and 2 (MRP1, MRP2) mRNA in human colorectal adenocarcinomas.
  
  
  METHODS
  Colorectal adenocarcinomas were obtained as surgical samples from 25 patients. The chemosensitivity of 12 anticancer drugs was assessed by the collagen gel droplet embedded culture drug sensitivity test (CD-DST). The expression levels of MDR1, MRP1, and MRP2 mRNA in colorectal adenocarcinomas were also evaluated by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  The chemosensitivity was successfully evaluated for 16 of 25 patients, and the anticancer drugs were effective against the samples showing a relatively high growth rate. Gemcitabine hydrochloride was found to be more promising than those often prescribed for the treatment of colorectal adenocarcinoma. There was no correlation of the mRNA expression levels of MDR1 and MRP1 with the chemosensitivity of any anticancer drugs tested, but mitomycin C was found to be more effective for the colorectal adenocarcinoma with relatively high expression of MRP2 mRNA.
authors.affiliation: Division of Diabetes, Digestive, and Kidney Diseases, Department of Clinical Molecular Medicine, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Nakahara
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Sakaeda
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nakamura
authors.forename: Takao
authors.initials: T
authors.lastname: Tamura
authors.forename: Chiharu
authors.initials: C
authors.lastname: Nishioka
authors.forename: Nobuo
authors.initials: N
authors.lastname: Aoyama
authors.forename: Noboru
authors.initials: N
authors.lastname: Okamura
authors.forename: Toshiro
authors.initials: T
authors.lastname: Shirakawa
authors.forename: Akinobu
authors.initials: A
authors.lastname: Gotoh
authors.forename: Takashi
authors.initials: T
authors.lastname: Kamigaki
authors.forename: Masakazu
authors.initials: M
authors.lastname: Ohno
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kuroda
authors.forename: Masafumi
authors.initials: M
authors.lastname: Matsuo
authors.forename: Masato
authors.initials: M
authors.lastname: Kasuga
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Okumura
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D000230:Adenocarcinoma / Q000378:metabolism; D003094:Collagen; D006801:Humans; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D003094:Collagen; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D012333:RNA, Messenger