Chemokine receptors and early activation markers in acute anterior uveitis.
OBJECTIVE
To study the peripheral expression of chemokine receptors and early activation markers in acute anterior uveitis (AU).


METHODS
The expression of chemokine receptors CCR1, CCR2, CCR3, CCR5 and CXCR3, and activation markers CD25 (IL2R-alpha-chain), CD122 (IL2R-beta-chain) and CD69 were studied on peripheral blood mononuclear cells using flow cytometry in 10 patients with acute anterior uveitis and in 10 healthy controls.


RESULTS
We found a highly significant up-regulation of CCR5 (p < 0.001) on CD4+ T-cells in the blood of patients with AU compared to in healthy controls. CD69 was significantly higher on both CD4+ and CD8+ T-cells (p < 0.05 and p < 0.001, respectively). We also found a significantly higher expression of CD25 expressing CD4+ T-cells in patients with anterior uveitis (p < 0.05). This expression was directly correlated to the severity of disease (rho = 0.720, p < 0.05).


CONCLUSIONS
Our data show that patients with acute anterior uveitis have a specific systemic immune activation.
Klitgaard
Torben L Sørensen
TL
Department of Ophthalmology, Herlev Hospital, University of Copenhagen, Herlev, Denmark. torbenls@dadlnet.dk
Øgard
Carsten
C
Krogh
Erik
E
D000208:Acute Disease; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D037181:Lectins, C-Type; D008297:Male; D008875:Middle Aged; D019707:Receptors, Chemokine / Q000378:metabolism*; D015375:Receptors, Interleukin-2 / Q000378:metabolism*; D015854:Up-Regulation; D014606:Uveitis, Anterior / Q000378:metabolism*; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D037181:Lectins, C-Type; D019707:Receptors, Chemokine; D015375:Receptors, Interleukin-2; D000099192:CD69 Antigens