Adenine nucleotide translocase 3 (ANT3) overexpression induces apoptosis in cultured cells.
Mitochondrial adenine nucleotide translocase 1 (ANT1), but not ANT2, can dominantly induce apoptosis. Nothing is known, however, about the apoptotic activity of ANT3. We have transfected HeLa cells with the three human ANT isoforms to compare their potential as inducers of apoptosis. Transient overexpression of ANT3 resulted, like ANT1, in apoptosis as shown by an increase in the sub-G1 fraction, annexin V staining, low DeltaPsi(m), and activation of caspases 9 and 3. Moreover, the apoptosis produced by ANT3 was inhibited by bongkrekic acid and by cyclosporin A. The pro-apoptotic activities of the ANT1 and ANT3 isoforms contrast with the lack of apoptotic activity of ANT2. This finding may help to identify the specific factors associated with the pro-apoptotic activities of ANT isoforms.
Zamora
Mònica
M
Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Diagonal 645, E-08028 Barcelona, Spain.
Granell
Meritxell
M
Mampel
Teresa
T
Viñas
Octavi
O
D033781:Adenine Nucleotide Translocator 3 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects*; D001865:Bongkrekic Acid / Q000494:pharmacology; D020169:Caspases / Q000378:metabolism; D016572:Cyclosporine / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D008564:Membrane Potentials; D008928:Mitochondria / Q000737:chemistry / Q000502:physiology; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014162:Transfection
D033781:Adenine Nucleotide Translocator 3; D004791:Enzyme Inhibitors; D020033:Protein Isoforms; D001865:Bongkrekic Acid; D016572:Cyclosporine; D020169:Caspases