title: [Chromatin immunoprecipitation and its preliminary application for gene regulation].
abstract: OBJECTIVE
  To verify the binding of p53 to p21WAF1/CIP1 gene promoter and detect its binding to hsp90 beta gene promoter in vivo.
  
  
  METHODS
  Chromatin immunoprecipitation and PCR analysis were used to measure specific gene regulation sequence and Western blot analysis to investigate p53 protein.
  
  
  RESULTS
  The p53 binding sequences on the promoters of p21WAF1/CIP1 and hsp90 beta gene were found in the p53 antibody immunoprecipitated DNA fragments and p53 was detected in the immunoprecipitated samples.
  
  
  CONCLUSIONS
  p53 binds to promoters of p21WAF1/CIP1 and hsp90 beta gene in vivo, and regulates the expression of the two genes.
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiao-kuan
authors.initials: XK
authors.lastname: Cheng
authors.forename: Ning-hua
authors.initials: NH
authors.lastname: Wu
authors.forename: Yu-fei
authors.initials: YF
authors.lastname: Shen
mesh_terms: D001665:Binding Sites; D002843:Chromatin / Q000235:genetics* / Q000302:isolation & purification; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011233:Precipitin Tests / Q000379:methods; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014158:Transcription, Genetic; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism / Q000502:physiology
chemical_list: C493956:CDKN1A protein, human; D002843:Chromatin; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D018841:HSP90 Heat-Shock Proteins; D016159:Tumor Suppressor Protein p53