Radio-high-performance liquid chromatography for ecotoxicity assessment of insect growth regulators.
The described radiochromatographic method permits fast and high-sensitivity monitoring of soil biodegradation products of an insect growth regulator for its environmental risk assessment. We analyzed and compared two diastereoisomers of ethyl N-(2-(4-[(2-hydroxycyclohexyl) methyl]phenoxy)ethyl)carbamate, namely its cis-(1S,2S) isomer JN-W330 and a trans-(1R,2S) isomer JN-W331. Microbial conversion of the cis-isomer to the trans-isomer was proved by mass spectrometry analyzer. Among the chromatographic columns tested, the best separation was found with a 125 mm x 4 mm i.d. column packed with Supersphere 100 RP-C18, 5 microm and an acetonitrile-water gradient. The detection limit for the both isomers was in the range of 120-250 Bq (0.3-0.8 ng) at a concentration of 2 ng/ml with radiometric detection. The calibration curves for standard solutions were linear in the range of 150Bq-150kBq (r = 0.996). The method enabled us to compare the analyzed juvenoids with biologically active oostatic peptides in terms of their environmental safety.
Tykva
Richard
R
Institute of Organic Chemistry and Biochemistry, Academy of Sciences, CZ-16610 Prague 6, Czech Republic. tykva@uochb.cas.cz
Vlasáková
Vĕra
V
Novák
Jaroslav
J
Havlícek
Libor
L
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007605:Juvenile Hormones / Q000737:chemistry / Q000633:toxicity*; D011874:Radiometry / Q000379:methods*; D013237:Stereoisomerism
D007605:Juvenile Hormones