[A study on changes of myosin ATPase activities in cardiac ischemic reperfusion injury of rabbit].
OBJECTIVE
To investigate the changes of myosin ATPase activities in cardiac ischemic reperfusion injury of rabbit.


METHODS
A convenient method was adopted to purify the myosin from the left ventricle of normal rabbit heart and of cardiac ischemic reperfusion injured rabbit heart. The michaelis constant (K(m)) and maximum velocity(Vmax) values of Ca(2+)-activated ATPase were determined from the rate of Pi release in enzymatic reaction. The myosin ATPase activities of normal group and ischemic group were compared.


RESULTS
The myosin ATPase activities of the control group were Vmax: (1.15 +/- 0.17) mumol/(mg.min), K(m): (5.43 +/- 2.18) mmol and those of ischemic group were Vmax: (1.17 +/- 0.21) mumol/(mg.min), K(m): (6.02 +/- 2.01) mmol. There was no significant difference between the two groups(P > 0.05).


CONCLUSIONS
This modelling animal experiment showed that the myosin ATPase activities were unchanged in cardiac ischemic reperfusion injury.
Zhu
Bin
B
Department of Biomedical Engineering, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Wan
Chao-min
CM
Guo
Hui-ling
HL
Xiao
Jing
J
Wang
Zheng-rong
ZR
D000818:Animals; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D009218:Myosins / Q000378:metabolism*; D011817:Rabbits; D011897:Random Allocation
D009218:Myosins