[In vitro gene transfection by magnetic iron oxide nanoparticles and magnetic field increases transfection efficiency].
OBJECTIVE
To evaluate the feasibility of using iron oxide nanoparticles as gene vector and the effect of magnetic field on efficiency of transfection.


METHODS
Iron oxide nanoparticles were prepared by alkaline precipitation of divalent and trivalent iron chloride. The surface of iron oxide nanoparticles was modified by self-assembled poly-L-lysine to form particle complexes (IONP-PLL). Transfection was determined by delivering reporter gene, PGL2-control encoding luciferase, to different cell lines using IONP-PLL as vector. The effect of magnetic field on efficiency of transfection was determined using Nd-Fe-B permanent magnet.


RESULTS
Foreign gene could be delivered to various cell lines by IONP-PLL and expressed with high efficiency, but the transfection efficiency and time course varied in the different cell lines studied. Magnetic field could enhance the efficiency of transfection by 5 - 10 fold.


CONCLUSIONS
IONP-PLL can be used as a novel non-viral gene vector in vitro, which offers a basis for gene delivery in vivo.
Xiang
Juan-juan
JJ
Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Nie
Xin-min
XM
Tang
Jing-qun
JQ
Wang
Yan-jin
YJ
Li
Zheng
Z
Gan
Kai
K
Huang
He
H
Xiong
Wei
W
Li
Xiao-ling
XL
Li
Gui-yuan
GY
D000818:Animals; D019556:COS Cells; D005290:Ferric Compounds / Q000008:administration & dosage*; D005822:Genetic Vectors; D008280:Magnetics; D011107:Polylysine / Q000008:administration & dosage*; D014162:Transfection / Q000379:methods*
D005290:Ferric Compounds; C000499:ferric oxide; D011107:Polylysine