title: Isolation and characterization of polybrominated diphenyl ethers as inhibitors of microtubule assembly from the marine sponge Phyllospongia dendyi collected at Palau.
abstract: Two new polybrominated diphenyl ethers (1 and 2) were isolated by bioassay-guided separations together with nine known compounds (3-11) from the marine sponge Phyllospongia dendyi collected from Palau. The structures were assigned on the basis of their spectral data. Compounds 3, 7, and 10 showed inhibitory activities to the assembly of microtubule proteins (IC(50): 29.6, 33.5, and 20.9 microM, respectively) and to the meiotic maturation of starfish oocytes (IC(50): 3.6, 4.2, and 4.2 microM, respectively), while 1, 2, 4-6, 8, 9, and 11 were not active at 100 microM. Two phenolic hydroxyls are required for their bioactivities.
authors.affiliation: Department of Ocean Sciences, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Liu
authors.forename: Michio
authors.initials: M
authors.lastname: Namikoshi
authors.forename: Shiori
authors.initials: S
authors.lastname: Meguro
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nagai
authors.forename: Hisayoshi
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Yao
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004987:Ethers / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D008870:Microtubules / Q000187:drug effects* / Q000378:metabolism*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D009792:Oceans and Seas; D009865:Oocytes / Q000187:drug effects; D011075:Polybrominated Biphenyls / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011161:Porifera / Q000737:chemistry*; D013215:Starfish / Q000187:drug effects
chemical_list: D004224:Diterpenes; D004987:Ethers; D011075:Polybrominated Biphenyls