title: Novel and deviant Walker A ATP-binding motifs in bacteriophage large terminase-DNA packaging proteins.
abstract: Bacteriophage terminases constitute a very interesting class of viral-coded multifunctional ATPase "motors" that apparently drive directional translocation of DNA into an empty viral capsid. A common Walker A motif and other conserved signatures of a critical ATPase catalytic center are identified in the N-terminal half of numerous large terminase proteins. However, several terminases, including the well-characterized lambda and SPP1 terminases, seem to lack the classic Walker A in the N-terminus. Using sequence alignment approaches, we discovered the presence of deviant Walker A motifs in these and many other phage terminases. One deviation, the presence of a lysine at the beginning of P-loop, may represent a 3D equivalent of the universally conserved lysine in the Walker A GKT/S signature. This and other novel putative Walker A motifs that first came to light through this study help define the ATPase centers of phage and viral terminases as well as elicit important insights into the molecular functioning of this fundamental motif in biological systems.
authors.affiliation: Department of Biology, The Catholic University of America, Washington, DC 20064, USA.
authors.forename: Michael S
authors.initials: MS
authors.lastname: Mitchell
authors.forename: Venigalla B
authors.initials: VB
authors.lastname: Rao
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D020816:Amino Acid Motifs / Q000235:genetics; D000595:Amino Acid Sequence; D010582:Bacteriophage lambda / Q000201:enzymology / Q000235:genetics*; D004706:Endodeoxyribonucleases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014644:Genetic Variation; D016679:Genome, Viral; D008239:Lysine; D008969:Molecular Sequence Data; D016415:Sequence Alignment
chemical_list: D000255:Adenosine Triphosphate; D004706:Endodeoxyribonucleases; C030894:terminase; D008239:Lysine