[Radiological study on repair of goat tibia defect with marrow stromal stem cells and bio-derived bone].
OBJECTIVE
To investigate the ability to repair goat tibia defect with marrow stromal stem cells (MSCs) and bio-derived bone, and the feasibility of the compounds as bone substitute material.


METHODS
MSCs were cultured with the bio-derived bone in vitro, and the 20 mm tibia defect of goat was made and fixed with plate. Eighteen goats were divided into experimental group, control group and blank group. The defects were not filled with anything in blank group, with tissue engineering bone in experimental group and bio-derived bone in control group. The repair capability was assessed by physical, X-ray and bone mineral density examinations 8, 12, 16, and 24 weeks after operation.


RESULTS
In experimental group, the defects were partially repaired 8 weeks, and completely repaired 12 and 16 weeks; there was significant difference in bone density between experimental group and control group (P < 0.05) 8, 12 and 16 weeks, but no significant difference 24 weeks. The defects of blank group were not repaired 24 weeks.


CONCLUSIONS
The tissue engineering bone can efficiently repair bone defect, and its repair capability is better than that of bio-derived bone alone both in quantity and quality of bone formation.
Qi
Chao
C
Division of Stem Cell and Tissue Engineering, Key Laboratory of Biotherapy of Human Diseases Ministry of Education, West China Hospital, Sichuan University, Chengdu, Sichuan, P. R. China 610041.
Huang
Fu-guo
FG
Yang
Zhi-ming
ZM
D000818:Animals; D001672:Biocompatible Materials; D001854:Bone Marrow Cells / Q000166:cytology*; D002478:Cells, Cultured; D005260:Female; D006041:Goats; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D010012:Osteogenesis; D011859:Radiography; D013977:Tibia / Q000000981:diagnostic imaging / Q000293:injuries* / Q000601:surgery; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials