title: Analysis of mutational lineage trees from sites of primary and secondary Ig gene diversification in rabbits and chickens.
abstract: Lineage trees of mutated rearranged Ig V region sequences in B lymphocyte clones often serve to qualitatively illustrate claims concerning the dynamics of affinity maturation. In this study, we use a novel method for analyzing lineage tree shapes, using terms from graph theory to quantify the differences between primary and secondary diversification in rabbits and chickens. In these species, Ig gene diversification starts with rearrangement of a single (in chicken) or a few (in rabbit) V(H) genes. Somatic hypermutation and gene conversion contribute to primary diversification in appendix of young rabbits or in bursa of Fabricius of embryonic and young chickens and to secondary diversification during immune responses in germinal centers (GCs). We find that, at least in rabbits, primary diversification appears to occur at a constant rate in the appendix, and the type of Ag-specific selection seen in splenic GCs is absent. This supports the view that a primary repertoire is being generated within the expanding clonally related B cells in appendix of young rabbits and emphasizes the important role that gut-associated lymphoid tissues may play in early development of mammalian immune repertoires. Additionally, the data indicate a higher rate of hypermutation in rabbit and chicken GCs, such that the balance between hypermutation and selection tends more toward mutation and less toward selection in rabbit and chicken compared with murine GCs.
authors.affiliation: Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel. mehrra@mail.biu.ac.il
authors.forename: Ramit
authors.initials: R
authors.lastname: Mehr
authors.forename: Hanna
authors.initials: H
authors.lastname: Edelman
authors.forename: Devinder
authors.initials: D
authors.lastname: Sehgal
authors.forename: Rose
authors.initials: R
authors.lastname: Mage
mesh_terms: D000465:Algorithms; D000818:Animals; D000916:Antibody Diversity / Q000235:genetics*; D001065:Appendix / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D002060:Bursa of Fabricius / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D019070:Cell Lineage / Q000235:genetics / Q000276:immunology; D002645:Chickens; D002999:Clone Cells; D004252:DNA Mutational Analysis / Q000379:methods; D005785:Gene Conversion / Q000276:immunology; D005803:Genes, Immunoglobulin; D018858:Germinal Center / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics; D011817:Rabbits; D027041:Somatic Hypermutation, Immunoglobulin
chemical_list: D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains