title: Anorexigenic C75 alters c-Fos in mouse hypothalamic and hindbrain subnuclei.
abstract: The fatty acid synthase inhibitor C75 reduces feeding rapidly and for several days. We investigated brain sites potentially involved in actions of i.p. C75 in mice by examining c-Fos. At 3 h C75 increased numbers of c-Fos-immunoreactive cells in hindbrain feeding-related nuclei, and in the paraventricular nucleus (PVN), lateral aspects of the arcuate nucleus (ARC), and in the central amygdala. At 24 h C75 prevented fasting-induced c-Fos expression in the medial ARC and three of its targets: lateral magnocellular PVN, lateral hypothalamus, and dorsomedial hypothalamus. C75, but not fasting, increased c-Fos in parvocellular PVN. This pattern of results suggests a shift from hindbrain-initiated short-term actions to activation of hypothalamic mechanisms that could mediate the long-term anorectic responses to C75.
authors.affiliation: Departments of Neuroscience and Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Ian
authors.initials: I
authors.lastname: Miller
authors.forename: Gabriele V
authors.initials: GV
authors.lastname: Ronnett
authors.forename: Timothy H
authors.initials: TH
authors.lastname: Moran
authors.forename: Susan
authors.initials: S
authors.lastname: Aja
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002452:Cell Count / Q000379:methods; D004435:Eating / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005215:Fasting / Q000378:metabolism; D007031:Hypothalamus / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D012249:Rhombencephalon / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors
chemical_list: C406734:4-methylene-2-octyl-5-oxofuran-3-carboxylic acid; D004791:Enzyme Inhibitors; D016760:Proto-Oncogene Proteins c-fos; D015107:4-Butyrolactone