Distribution of CD8 and CD20 lymphocytes in chronic periapical inflammatory lesions.
The objective of this study was to investigate the distribution of CD8+ and CD20+ lymphocytes in chronic periapical inflammatory lesions. A total of 90 periapical inflammatory lesions (chronic abscesses, abscessed cysts, and inflammatory cysts) were evaluated. The biotin-streptavidin immunohistochemical technique was used to identify cytotoxic/suppressor T-lymphocytes (CD8) and B-lymphocytes (CD20). Age ranged from 10 to 67 years. Patients between 26 and 45 years old (54.4%), females (52.2%), and white patients (74.4%) were more frequently affected. CD8+ cell distribution was as follows: 1) fibrous capsule: diffuse in 58.8% of chronic abscesses and absent in 64.1% of abscessed cysts and in 70.6% of inflammatory cysts; 2) infiltration zone: diffuse in 100% of abscessed cysts and in 82.4% of inflammatory cysts; 3) sub-epithelial zone: absent in 53.0% of inflammatory cysts and diffuse in 56.4% of abscessed cysts; 4) suppurative zone: diffuse in 100% of chronic abscesses and in 97.5% of abscessed cysts. CD20+ cell distribution was as follows: 1) fibrous capsule: absent in 100% of inflammatory cysts, in 94.8% of abscessed cysts, and in 88.3% of chronic abscesses; 2) infiltration zone: diffuse in 100% of abscessed cysts and in 53% of inflammatory cysts; 3) sub-epithelial zone: absent in 58.8% of inflammatory cysts and focal in 46.2% of abscessed cysts; 4) suppurative zone: diffuse in 100% of abscessed cysts and in 100% of chronic abscesses. The distribution of the lymphocytic infiltrate in the lesions was usually diffuse for both types of lymphocytes.
Philippi
Christine Kalvelage
CK
Department of Oral Pathology, School of Dentistry, UNIVALI, Itajaí, SC, Brazil.
Rados
Pantelis Varvaki
PV
Sant'ana Filho
Manoel
M
Barbachan
João Jorge Diniz
JJ
Quadros
Onofre Francisco de
OF
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D018951:Antigens, CD20 / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology*; D016827:CD8 Antigens / Q000032:analysis; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000473:pathology*; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D002648:Child; D002908:Chronic Disease; D003238:Connective Tissue / Q000276:immunology / Q000473:pathology; D004848:Epithelium / Q000276:immunology / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010482:Periapical Abscess / Q000276:immunology / Q000473:pathology; D010485:Periapical Periodontitis / Q000276:immunology* / Q000473:pathology; D011842:Radicular Cyst / Q000276:immunology / Q000473:pathology; D012737:Sex Factors; D013492:Suppuration; D013602:T-Lymphocytes, Cytotoxic / Q000473:pathology; D044465:White People; D055815:Young Adult
D018951:Antigens, CD20; D016827:CD8 Antigens