Protein-protein interaction analysis by nuclear magnetic resonance spectroscopy.
Nuclear magnetic resonance (NMR) is a powerful technique to study protein-protein interactions in solution. Various methods have been developed and applied successfully for locating binding sites on proteins. The application of NMR chemical-shift perturbation to map the protein-protein interfaces is described in this chapter, providing a practical guideline that can be followed to carry out the experiments.
Gao
Guanghua
G
Department of Biochemistry and Biophysics, University of North Carolina @ Chapel Hill, Chapel Hill, NC, USA.
Williams
Jason G
JG
Campbell
Sharon L
SL
D001665:Binding Sites; D006801:Humans; D046911:Macromolecular Substances; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*
D046911:Macromolecular Substances; D011506:Proteins