Production of highly 13C-labeled polyphenols in Vitis vinifera cell bioreactor cultures.
The use of Vitis vinifera cells grown in a 2 l-stirred tank bioreactor for producing isotopically 13C-labeled phenolic substances is presented. Several culture parameters were optimized to achieve characteristics of growth and polyphenol metabolism similar to that recorded in shake flasks. Administration of [1-13C]L-phenylalanine (3 mM) to grape cell suspension cultures led to the production of 13C-labeled stilbenes (trans- and cis-piceids), catechins (catechin and epicatechin) and anthocyanins (delphinidin-, cyanidin-, petunidin-, peonidin- and malvidin-3-O-beta-glucosides). Incorporation of [1-13C]L-phenylalanine into polyphenols was measured by means of 13C satellites in the proton NMR spectrum and EA-IRMS. The enrichment of labeling obtained for all the compounds (between 40 and 65%) is sufficient to investigate their absorption and metabolism in humans.
Aumont
Virginie
V
Groupe d'Etudes des Substances Végétales à Activité Biologique, EA 3675, Université de Bordeaux 2, Faculté des Sciences Pharmaceutiques, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France.
Larronde
Fabienne
F
Richard
Tristan
T
Budzinski
Hélène
H
Decendit
Alain
A
Deffieux
Gérard
G
Krisa
Stéphanie
S
Mérillon
Jean-Michel
JM
D019149:Bioreactors; D002247:Carbon Isotopes; D005419:Flavonoids / Q000096:biosynthesis*; D007553:Isotope Labeling / Q000379:methods*; D010636:Phenols; D010649:Phenylalanine / Q000378:metabolism; D059808:Polyphenols; D013267:Stilbenes / Q000378:metabolism; D027843:Vitis / Q000166:cytology* / Q000378:metabolism*
D002247:Carbon Isotopes; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D013267:Stilbenes; D010649:Phenylalanine