[Inhibitory effect of isorhmnetin on telomerase activity of HeLa cells].
OBJECTIVE
To investigate the growth-inhibiting and apoptosis-inducing effects of isorhmnetin on HeLa cells and to disclose the role of telomerase activity of tumor cells.


METHODS
The methods of cell culture in vitro were adopted. HeLa cells were treated with isorhmnetin in different concentrations for 2 days, and then were observed and analyzed by use of MTT, Flow-Cytometry (FCM) and TRAP-ELIAS technique for inspecting the HeLa cells' growth and telomerase activity.


RESULTS
The growth of HeLa cells was inhibited evidently after treatment by isorhmnetin. The rate of apoptosis was 31.7% after the HeLa cells were treated with 20 micrograms/ml isorhmnetin. Isorhmnetin could inhibit the activity of telomerase.


CONCLUSIONS
By inhibiting the activity of telomerase, isorhmnetin can inhibit the growth of HeLa cells.
Yang
Chun-lei
CL
Department of Cell Biology, Life Science College, Sichun University, Chengdu 610041, China.
Qu
Yi
Y
Wang
Zheng-rong
ZR
Tao
Da-chang
DC
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D044948:Flavonols / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D011794:Quercetin / Q000031:analogs & derivatives; D019098:Telomerase / Q000187:drug effects / Q000378:metabolism*
D000972:Antineoplastic Agents, Phytogenic; D044948:Flavonols; C047368:3-methylquercetin; D011794:Quercetin; D019098:Telomerase