title: Design and evaluation of a specific primer pair for the diagnosis and identification of Acanthamoeba polyphaga.
abstract: Random amplified polymorphism DNA (RAPD) is a useful tool for species identification. The obtained band patterns can be used for specific primer pair design that may be useful for species diagnosis. In this study, a distinctive a 962-bp band in A. polyphaga band patterns was found, by using the OPC20 primer (ACTTCGCCAC). The DNA fragment was used to design a specific primer pair that was useful for the identification of different isolates as A. polyphaga species. A case of A. polyphaga in disseminated acanthamoebiasis affecting mesenteric nodes is also reported.
authors.affiliation: Department of Parasitology, Faculty of Pharmacy, University of La Laguna, Avda. Francisco Sánchez s/n, C.P. 38271, La Laguna, Tenerife, Spain. aortega@ull.es
authors.forename: Antonio
authors.initials: A
authors.lastname: Ortega-Rivas
authors.forename: Jacob
authors.initials: J
authors.lastname: Lorenzo-Morales
authors.forename: Enrique
authors.initials: E
authors.lastname: Martinez-Carretero
authors.forename: Mercedes
authors.initials: M
authors.lastname: Villa Lloberas
authors.forename: Basilio
authors.initials: B
authors.lastname: Valladares Hernández
authors.forename: Antonio
authors.initials: A
authors.lastname: del Castillo-Remiro
mesh_terms: D000048:Acanthamoeba / Q000235:genetics* / Q000302:isolation & purification*; D000562:Amebiasis / Q000175:diagnosis* / Q000209:etiology; D000818:Animals; D016172:DNA Fingerprinting; D017931:DNA Primers; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D008198:Lymph Nodes / Q000469:parasitology; D008643:Mesentery / Q000469:parasitology; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D016054:DNA, Protozoan