Expression of tropomyosin-encoding gene in the kidney depends on functioning of vasopressin gene.
Brattleboro diabetes insipidus mutant rats and normal WAG rats were subjected to water loading or thirst during 3 days. It was found that tropomyosin-encoding gene expression has a tissue-specific pattern in the kidney. Northern blot and western blot analysis had shown that the main expression of Tpm3(3) takes place in the renal medulla, and its intensity differs in normal and mutant rats. The differences between mutants with an ineffective vasopressin synthesizing system and the rats having an intact vasopressin gene were more distinct under long-lasting dehydration. The ratio between renal medullary tropomyosin of Brattleboro and WAG lines of rats was 39.76 +/- 0.90 versus 18.29 +/- 0.86 under water loading, and 46.12 +/- 2.14 versus 13.83 +/- 0.66 in thirst.
Khegay
Igor I
II
Siberian Department, Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, Russia. khegay@bionet.nsc.ru
Katokhin
Alexey V
AV
D000818:Animals; D003919:Diabetes Insipidus / Q000235:genetics; D005786:Gene Expression Regulation; D007668:Kidney / Q000502:physiology*; D007679:Kidney Medulla / Q000502:physiology; D008297:Male; D051381:Rats; D011910:Rats, Brattleboro; D011919:Rats, Inbred Strains; D012016:Reference Values; D013894:Thirst / Q000502:physiology; D014335:Tropomyosin / Q000235:genetics* / Q000378:metabolism; D014667:Vasopressins / Q000235:genetics*
C498784:Tpm3 protein, rat; D014335:Tropomyosin; D014667:Vasopressins