Construction of a high-biomass, iron-enriched yeast strain and study on distribution of iron in the cells of Saccharomyces cerevisiae.
A high-biomass, iron-enriched Saccharomyces cerevisiae ZYF-15 was constructed by interspecies protoplast fusion. Under optimal fermentation condition, the biomass and iron content of the strain reached 11 g l(-1) and 25 mg Fe g(-1) dry cells, respectively. About 96% of enriched iron is converted into organic iron, which is mainly in cell walls and vacuoles with some bound to DNA, RNA and protein.
Yuan
Yulan
Y
College of Life Science, Sichuan University, Chengdu 610064, P.R. China.
Guo
Xuena
X
He
Xiuping
X
Zhang
Borun
B
Liu
Shigui
S
D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D001711:Biotransformation; D004247:DNA / Q000378:metabolism; D005285:Fermentation; D007501:Iron / Q000378:metabolism*; D011523:Protoplasts / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism
D029701:Saccharomyces cerevisiae Proteins; D012313:RNA; D004247:DNA; D007501:Iron