A simple remedy against artifactual double bands in denaturing gradient gel electrophoresis.
Denaturant gradient gel electrophoresis (DGGE) is a widely used method for mutation analysis and for studies of microbial diversity. Particular combinations of target gene fragments and primers may give rise to erroneous DGGE profiles. We report on a very straightforward means to eliminate the artifactual 'double bands' that can be encountered in several applications.
Janse
Ingmar
I
NIOO-KNAW, Department of Microbial Ecology, Centre for Limnology, Rijksstraatweg 6, 3631 AC Nieuwersluis, The Netherlands. i.janse@nioo.knaw.nl
Bok
Jasper
J
Zwart
Gabriel
G
D016477:Artifacts; D000458:Cyanobacteria / Q000235:genetics / Q000302:isolation & purification; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005189:False Positive Reactions; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction / Q000379:methods*; D014871:Water Microbiology
D004269:DNA, Bacterial