L-selectin defines a bone marrow analog to the thymic early T-lineage progenitor.
The recent description of an early T-lineage progenitor (ETP) population in adult mouse thymus implies the presence of a bone marrow predecessor that has not yet been identified. Here we describe a Lin(Neg) Sca-1(Pos) c-kit(Hi) Thy-1.1(Neg) L-selectin(Pos) adult mouse bone marrow population that resembles the thymic ETP in both antigen expression phenotype and posttransplantation lineage potential. These cells produce wavelike kinetics of thymic seeding and reconstitute the irradiated thymus with kinetics comparable to a thymocyte graft after intravenous transplantation. Transient B-lineage reconstitution is also observed, but little myeloid potential can be detected in transplant experiments. A second subset of progenitors is L-selectin(Neg) and is highly enriched for rapid and persistent T- and B-lineage potential, as well as some myeloid potential. L-selectin (CD62L) is therefore an effective marker for separating lymphoid progenitors from myeloid progenitors and hematopoietic stem cells in mouse bone marrow.
Perry
S Scott
SS
Department of Medicine, University of Utah School of Medicine, Salt Lake City 84132, USA.
Wang
Hongfang
H
Pierce
L Jeanne
LJ
Yang
Anne Marie
AM
Tsai
Schickwann
S
Spangrude
Gerald J
GJ
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism; D003114:Colony-Forming Units Assay; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D066298:In Vitro Techniques; D019041:L-Selectin / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D011828:Radiation Chimera; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism*
D019041:L-Selectin