title: TIA-1 expression in hairy cell leukemia.
abstract: We measured T-cell intracellular antigen-1 (TIA-1) expression in neoplastic cells from patients with hairy cell leukemia. Five of nine cases were positive for cytoplasmic TIA-1, with a small, dot-like, granular expression pattern. However, neoplastic cells were granzyme B- and perforin- negative in all cases. Other positive markers were CD20 in 9/9 cases, CD19 in 9/9 cases, DBA44 in 8/9 cases, LeuM5(CD11C) in 8/9 cases, IL-2R(CD25) in 7/9 cases, CD103 in 7/9 cases, FMC7 in 6/9 cases, and tartrate- resistant acid phosphatase in 5/7 cases. We also analyzed TIA-1 expression in 94 B cell lymphomas, including 19 diffuse, large cell lymphomas, 19 mantle cell lymphomas, six follicular lymphomas, two extranodal marginal zone B-cell lymphomas, 13 nodal marginal zone B-cell lymphomas, one mediastinal large-cell lymphoma, 19 diffuse small-cell lymphomas, 14 myelomas, and one splenic lymphoma with villous lymphocytes. All cases were negative for TIA-1 expression. Based on these findings, TIA-1 expression in neoplastic cells of low-grade B-cell lymphomas may be a good diagnostic marker for hairy cell leukemia. Moreover, TIA-1 reactivity in lymphomas does not necessarily indicate a T- or NK-cell derivation.
authors.affiliation: Department of Pathology of Biological Response, Nagoya University Graduate School of Medicine, Showa-ku, Nagoya, Japan. naoyoshi@med.nagoya-u.ac.jp
authors.forename: Naoyoshi
authors.initials: N
authors.lastname: Mori
authors.forename: Yoshiko Idate
authors.initials: YI
authors.lastname: Murakami
authors.forename: Satoko
authors.initials: S
authors.lastname: Shimada
authors.forename: Sachiko
authors.initials: S
authors.lastname: Iwamizu-Watanabe
authors.forename: Yoriko
authors.initials: Y
authors.lastname: Yamashita
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Hasegawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kojima
authors.forename: Toshiro
authors.initials: T
authors.lastname: Nagasawa
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D018941:Antigens, CD19 / Q000032:analysis; D018951:Antigens, CD20 / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis; D001854:Bone Marrow Cells / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D039521:CD11c Antigen / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D039001:Integrin alpha Chains / Q000032:analysis; D007943:Leukemia, Hairy Cell / Q000378:metabolism / Q000473:pathology*; D008297:Male; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D039102:Poly(A)-Binding Proteins; D014176:Protein Biosynthesis; D011506:Proteins; D016601:RNA-Binding Proteins; D000074019:T-Cell Intracellular Antigen-1
chemical_list: D015703:Antigens, CD; D018941:Antigens, CD19; D018951:Antigens, CD20; D014408:Biomarkers, Tumor; D039521:CD11c Antigen; D006023:Glycoproteins; D039001:Integrin alpha Chains; C112523:MS4A1 protein, human; D039102:Poly(A)-Binding Proteins; D011506:Proteins; D016601:RNA-Binding Proteins; D000074019:T-Cell Intracellular Antigen-1; C071558:TIA1 protein, human; C091367:alpha E integrins