title: Evaluation of porB PCR-amplicon restriction endonuclease analysis as a method to determine porB variable-region sequences in nonserotypeable meningococci.
abstract: porB PCR-amplicon restriction endonuclease analysis is a rapid, simple method developed to assess porB variation in nonserotypeable meningococci isolated during New Zealand's epidemic of meningococcal disease. Most nonserotypeable meningococci isolated between 1990 and 1999 inclusively either were type 4 (40.5%) or contained the porB variable region 1 (VR1)-19, VR2-D, VR3-7, and VR4-14a sequences (45.1%).
authors.affiliation: Communicable Disease Group, ESR Ltd., Porirua. Microbiology Department, University of Otago, Dunedin, New Zealand. kristin.dyet@esr.cri.nz
authors.forename: Kristin H
authors.initials: KH
authors.lastname: Dyet
authors.forename: Robin S
authors.initials: RS
authors.lastname: Simmonds
authors.forename: Diana R
authors.initials: DR
authors.lastname: Martin
mesh_terms: D000595:Amino Acid Sequence; D015373:Bacterial Typing Techniques; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004196:Disease Outbreaks; D014644:Genetic Variation; D006801:Humans; D008589:Meningococcal Infections / Q000453:epidemiology / Q000382:microbiology; D008969:Molecular Sequence Data; D009345:Neisseria meningitidis / Q000145:classification* / Q000235:genetics; D009520:New Zealand / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D018272:Porins / Q000235:genetics*; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D012703:Serotyping
chemical_list: D018272:Porins; C057699:porin protein, Neisseria; D004262:DNA Restriction Enzymes