Chemical syntheses of inhibitory substrates of the RNA-RNA ligation reaction catalyzed by the hairpin ribozyme.
The chemical syntheses of RNA oligomers containing modifications on the 5'-carbon of the 5'-terminal nucleoside for crystallographic and mechanistic studies of the hairpin ribozyme are reported. Phosphoramidites 4 and 8 were prepared and used in solid phase syntheses of RNA oligomers containing the sequence 5'-N'UCCUCUCC, where N' indicates either 5'-chloro-5'-deoxyguanosine or 5'-amino-5'-deoxyguanosine, respectively. A ribozyme ligation assay with the 5'-chloro- and 5'-amino-modified RNA oligomers demonstrated their inhibition of the hairpin-catalyzed RNA-RNA ligation reaction.
Massey
Archna P
AP
Department of Chemistry, University of Washington, Seattle, WA 98195-1700, USA.
Sigurdsson
Snorri Th
ST
D001483:Base Sequence; D002384:Catalysis; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D016337:RNA, Catalytic / Q000378:metabolism*; D012265:Ribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*
D009943:Organophosphorus Compounds; D016337:RNA, Catalytic; D012265:Ribonucleotides; C418481:hairpin ribozyme; C434331:phosphoramidite; D012313:RNA