title: Two sequences encoding chalcone synthase in yellow lupin (Lupinus luteus l.) may have evolved by gene duplication.
abstract: Two full copy cDNA sequences encoding chalcone synthase (CHS) were selected from a yellow lupin (Lupinus luteus L.) root and nodule cDNA library, and sequenced. Analysis of their open reading frames gave evidence that both encode the functional enzyme. Sequence alignment and phylogenetic studies on the DNA and protein level of these clones compared to the sequences of chalcone synthases from 54 other plant species reveal the possibility that lupin chalcone synthase is encoded by a multigene family consisting of at least two distinct genes that probably diverged by gene duplication. The duplication event is estimated to have taken place about 16 million years ago.
authors.affiliation: Department of Biochemistry and Biotechnology, August Cieszkowski Agricultural University of Poznań, Wołyńska 35, 60-637 Poznań, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Narozna
authors.forename: Jakub
authors.initials: J
authors.lastname: Paś
authors.forename: Jolanta
authors.initials: J
authors.lastname: Schneider
authors.forename: Cezary J
authors.initials: CJ
authors.lastname: Madrzak
mesh_terms: D000217:Acyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D020440:Gene Duplication; D015723:Gene Library; D017343:Genes, Plant; D031297:Lupinus / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D000217:Acyltransferases; C020141:flavanone synthetase