Purification of TNF binding proteins.
The finding that the two tumor necrosis factor receptors (TNFR) exist in soluble form in various body fluids not only has substantiated the paradigm of naturally existing soluble cytokine receptors but also has represented a milestone on the road to the biochemical and biological characterization of the two TNFRs. This chapter gives a simple, basic protocol for the purification of the two soluble TNFRs. The protocols found here may be easily adapted for the purification of various other soluble cytokine receptors. The purified proteins may be used in biological experiments or for the generation of specific research tools such as polyclonal or monoclonal antibodies.
Engelmann
Hartmut
H
Institute of Immunology, Munich University, Munchen, Germany.
Aderka
Dani
D
Wallach
David
D
D001671:Biochemistry / Q000379:methods*; D002352:Carrier Proteins / Q000302:isolation & purification*; D002460:Cell Line; D045744:Cell Line, Tumor; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D006801:Humans; D018124:Receptors, Tumor Necrosis Factor / Q000302:isolation & purification*; D047888:Receptors, Tumor Necrosis Factor, Type I; D053319:Tumor Necrosis Factor Decoy Receptors
D002352:Carrier Proteins; D018124:Receptors, Tumor Necrosis Factor; D047888:Receptors, Tumor Necrosis Factor, Type I; D053319:Tumor Necrosis Factor Decoy Receptors; C069521:recombinant human tumor necrosis factor-binding protein-1