Regulatory elements directing gut expression of the GATA6 gene during mouse early development.
The GATA6 transcription factor, as well as GATA4 and GATA5, is expressed in a variety of mammalian tissues including the precardiac mesoderm and endoderm, and gut-related organs. Genetic studies have also implicated GATA factors as important regulators of gut endoderm development. Previously, we identified the promoter and a cardiac-specific enhancer of mouse GATA6 [Sun-Wada et al. (2000) J. Biochem. 127, 703-709], however, little is known about the regulatory elements that govern GATA6 expression in the primitive gut. Here, we identified a distal enhancer of the GATA6 gene directing expression in the gut by creating transgenic mice. A sequence of approximately 200 bp between -8.0 kb and -7.8 kb contains element(s) that enhance transcription in the gut during embryonic development, when linked to the hsp68 promoter/lacZ fusion gene. Our results also show that GATA6 expression is controlled by multiple regulatory regions including cardiac-specific and gut-specific enhancers.
Sun-Wada
Ge-Hong
GH
Division of Biological Sciences, The Institute of Scientific and Industrial Research, Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047.
Kamei
Yoichi
Y
Wada
Yoh
Y
Futai
Masamitsu
M
D000818:Animals; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005314:Embryonic and Fetal Development; D050993:GATA6 Transcription Factor; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter / Q000502:physiology; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000737:chemistry / Q000196:embryology; D051379:Mice; D008822:Mice, Transgenic; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D004268:DNA-Binding Proteins; D050993:GATA6 Transcription Factor; C494759:GATA6 protein, human; C494760:Gata6 protein, mouse; D014157:Transcription Factors