title: Analysis of an engineered Salmonella flagellar fusion protein, FliR-FlhB.
abstract: Salmonella FliR and FlhB are membrane proteins necessary for flagellar export. In Clostridium a fliR-flhB fusion gene exists. We constructed a similar Salmonella fusion gene which is able to complement fliR, flhB, and fliR flhB null strains. Western blotting revealed that the FliR-FlhB fusion protein retains the FlhB protein's cleavage properties. We conclude that the FliR and FlhB proteins are physically associated in the wild-type Salmonella basal body, probably in a 1:1 ratio.
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA.
authors.forename: John S
authors.initials: JS
authors.lastname: Van Arnam
authors.forename: Jonathan L
authors.initials: JL
authors.lastname: McMurry
authors.forename: May
authors.initials: M
authors.lastname: Kihara
authors.forename: Robert M
authors.initials: RM
authors.lastname: Macnab
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D015153:Blotting, Western; D005407:Flagella / Q000378:metabolism*; D005816:Genetic Complementation Test; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics; D012475:Salmonella / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C085668:FlhB protein, Bacteria; C085248:FliR protein, Bacteria; D008565:Membrane Proteins