Standardization of MspI and HaeIII restriction karyotypes in cattle.
Cattle chromosomes were digested with two restriction endonucleases, MspI and HaeIII. The banding pattern induced by each enzyme allowed the identification and pairing of all individual chromosomes and consequently the elaboration of the karyotype. This method is rapid and technically easy, and proved to be of great utility in cattle cytogenetic studies.
Cymbron
T
T
Centro de Estudos de Ciência Animal, Instituto de Ciências Biomédicas Abel Salazar, Largo Abel Salazar No. 2, PT-4099-003 Porto, Portugal. tcymbron@yahoo.com.br
Chaves
R
R
Adega
F
F
Guedes-Pinto
H
H
I  Malheiro
M
M
D000818:Animals; D002417:Cattle; D002871:Chromosome Banding; D002875:Chromosomes / Q000201:enzymology* / Q000235:genetics*; D019003:Deoxyribonuclease HpaII / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics; D007621:Karyotyping
D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases