title: Canine and feline hematology reference values for the ADVIA 120 hematology system.
abstract: BACKGROUND
  The ADVIA 120 is a laser-based hematology analyzer with software applications for animal species. Accurate reference values would be useful for the assessment of new hematologic parameters and for interlaboratory comparisons.
  
  
  OBJECTIVE
  The goal of this study was to establish reference intervals for CBC results and new parameters for RBC morphology, reticulocytes, and platelets in healthy dogs and cats using the ADVIA 120 hematology system.
  
  
  METHODS
  The ADVIA 120, with multispecies software (version 1.107-MS), was used to analyze whole blood samples from clinically healthy dogs (n=46) and cats (n=61). Data distribution was determined and reference intervals were calculated as 2.5 to 97.5 percentiles and 25 to 75 percentiles.
  
  
  RESULTS
  Most data showed Gaussian or log-normal distribution. The numbers of RBCs falling outside the normocytic-normochromic range were slightly higher in cats than in dogs. Both dogs and cats had reticulocytes with low, medium, and high absorbance. Mean numbers of large platelets and platelet clumps were higher in cats compared with dogs.
  
  
  CONCLUSIONS
  Reference intervals obtained on the ADVIA 120 provide valuable baseline information for assessing new hematologic parameters and for interlaboratory comparisons. Differences compared with previously published reference values can be attributed largely to differences in methodology.
authors.affiliation: Clinic for Internal Medicine and Forensic Affairs, Faculty of Veterinary Medicine, Justus-Liebig-University, Giessen, Germany. andreas.moritz@vetmed.uni-giessen.de
authors.forename: Andreas
authors.initials: A
authors.lastname: Moritz
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Fickenscher
authors.forename: Karin
authors.initials: K
authors.lastname: Meyer
authors.forename: Klaus
authors.initials: K
authors.lastname: Failing
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Weiss
mesh_terms: D000818:Animals; D001772:Blood Cell Count / Q000379:methods / Q000592:standards / Q000662:veterinary*; D002415:Cats / Q000097:blood*; D004285:Dogs / Q000097:blood*; D004906:Erythrocyte Count / Q000379:methods / Q000592:standards / Q000662:veterinary; D005260:Female; D006403:Hematologic Tests / Q000295:instrumentation / Q000379:methods / Q000592:standards / Q000662:veterinary*; D008297:Male; D016011:Normal Distribution; D010976:Platelet Count / Q000379:methods / Q000592:standards / Q000662:veterinary; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity