title: [Development of toxin targeting to VEGF-KDR].
abstract: OBJECTIVE
  To develop toxin targeting vascular endothelial growth factor receptor II (VEGF-II/KDR) fused with a KDR-binling peptide screened from peptide library.
  
  
  METHODS
  By affinity to KDR molecular which expressed specifically by new born vascular endothelial cell, peptides to KDR were screened from C7 peptide library by phage display. Among them, a peptide binding to KDR with high affinity termed as P5 was selected and fused to the N-terminal of Shiga toxin subunit A (StxA). The protein (P5-StxA) was expressed in E. coli.
  
  
  RESULTS
  ELISA and Western blot were applied to characterize the binding interaction between the fusion protein, P5-StxA and KDR. Cytotoxicity assay showed that P5-StxA maintained similar toxicity to cell as StxA. In the model of angiogenesis, P5-StxA inhibited selectively VEGF-induced growth of preexisting vessels of the chick chorioallantoic membrane.
  
  
  CONCLUSIONS
  These studies demonstrate the small peptide, P5, maybe be used as carrier of toxin targeting to KDR.
authors.affiliation: Medical Research Department, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.forename: Hui-peng
authors.initials: HP
authors.lastname: Chen
authors.forename: Hong-bin
authors.initials: HB
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Tai-cheng
authors.initials: TC
authors.lastname: Yang
authors.forename: Jiang
authors.initials: J
authors.lastname: Xian
authors.forename: Chuan-hong
authors.initials: CH
authors.lastname: Yang
authors.forename: Huang-wen
authors.initials: HW
authors.lastname: Lai
authors.forename: Qing-ming
authors.initials: QM
authors.lastname: Wang
authors.forename: Wen-ling
authors.initials: WL
authors.lastname: Zheng
mesh_terms: D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D019151:Peptide Library; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D022621:Shiga Toxin / Q000378:metabolism*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism*
chemical_list: D019151:Peptide Library; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; D022621:Shiga Toxin; D040301:Vascular Endothelial Growth Factor Receptor-2