Dominant character of the molecular marker of a Biomphalaria tenagophila strain (Mollusca: Planorbidae) resistant to Schistosoma mansoni.
Biomphalaria tenagophila population from Taim (state of Rio Grande do Sul, Brazil) is totally resistant to Schistosoma mansoni, and presents a molecular marker of 350 bp by polymerase chain reaction and restriction fragment length polymorphism of the entire rDNA internal transcriber spacer. The scope of this work was to determine the heritage pattern of this marker. A series of cross-breedings between B. tenagophila from Taim (resistant) and B. tenagophila from Joinville, state of Santa Catarina (susceptible) was carried out, and their descendants F1 and F2 were submitted to this technique. It was possible to demonstrate that the specific fragment from Taim is endowed with dominant character, since the obtained segregation was typically mendelian.
Rosa
Florence Mara
FM
Departamento de Parasitologia, Instituto de Ciências Biológicas, UFMG, Belo Horizonte, MG, Brasil.
Caldeira
Roberta Lima
RL
Carvalho
Omar dos Santos
Odos S
Godard
Ana Lúcia Brunialti
AL
Coelho
Paulo Marcos Zech
PM
D000818:Animals; D001700:Biomphalaria / Q000235:genetics* / Q000469:parasitology; D001938:Brazil; D001947:Breeding / Q000379:methods*; D003433:Crosses, Genetic; D004275:DNA, Ribosomal / Q000032:analysis; D005799:Genes, Dominant / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D006790:Host-Parasite Interactions / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012550:Schistosoma mansoni
D004275:DNA, Ribosomal; D005819:Genetic Markers