[Study of interleukins and their soluble receptors in B-cell chronic lymphatic leukemia].
The purpose of our study was to examine concentrations of IL-1 beta, IL-1 beta R, IL-6 and IL-6R in blood plasma, culture supernatant and isolated broken lymphocytes in 20 patients with CLL, at I and III stage of the disease according to Rai's classification and in 10 healthy control subjects. The studies were carried out on the cultures of cells nonstimulated and stimulated with mitogene. A significant increase in IL-1 beta and IL-6 concentrations were found in all study groups during the course of B-CLL. The values of IL-1 beta R and IL-6R were increased in blood plasma at I and III stage of CLL and decreased in culture supernatants and broken lymphocytes before and after stimulation in comparison to control groups. In all cases studied parameters were higher after stimulation. In conclusion, significant increase of IL-1 beta and IL-6 concentrations during CLL may advocacy of higher synthesis and excretions of interleukins--stimulatores of cell proliferation by leukaemic lymphocytes. Increased IL-1 beta R and IL-6R concentrations in blood plasma during CLL, seems to be one of the mechanisms restricted access of IL-1 beta and IL-6 to their surface receptors. An increase of IL-1 beta and IL-6 concentrations and decrease of IL-1 beta R and IL-6R volues suggest survival of autoregulation mechanisms defended against autocrine excreted interleukins. The volues of concentrations of IL-1 beta and IL-1 beta R positively correlated with progress of disease. Such correlation was not found with respect to concentrations of IL-6 and IL-6R.
Parfieńczyk
Adam
A
Zakład Medycyny Nuklearnej Akademii Medycznej w Białymstoku.
Kiersnowska-Rogowska
Beata
B
Rogowski
Franciszek
F
D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D018450:Disease Progression; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood* / Q000473:pathology; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D017472:Receptors, Interleukin-1 / Q000097:blood*; D019947:Receptors, Interleukin-6 / Q000097:blood*; D012995:Solubility; D014407:Tumor Cells, Cultured
D007375:Interleukin-1; D015850:Interleukin-6; D017472:Receptors, Interleukin-1; D019947:Receptors, Interleukin-6