Mitochondrial DNA ligase in Crithidia fasciculata.
Kinetoplast DNA (kDNA), the form of mitochondrial DNA in trypanosomatids, consists of thousands of interlocked circular DNAs organized into a compact disk structure. A type II DNA topoisomerase, a DNA polymerase beta, and a structure-specific endonuclease have been localized to antipodal sites flanking the kDNA disk along with nascent DNA minicircles. We have cloned a gene (LIG k) encoding a mitochondrial DNA ligase in the trypanosomatid Crithidia fasciculata, and we show that an epitope-tagged form of the ligase colocalizes with the other replication proteins at the antipodal sites and also at the two faces of the kDNA disk. DNA LIG k becomes adenylated in reactions with ATP, and the adenylate moiety is removed by incubation with pyrophosphate or nicked DNA. The ligase interacts physically with the beta polymerase and is proposed to be involved in the repair of gaps in the newly synthesized minicircles. In yeast and mammals, a single gene encodes both nuclear and mitochondrial forms of DNA ligase. The LIG K protein sequence has low similarity to mitochondrial DNA ligases in other eukaryotes and is distinct from the C. fasciculata nuclear DNA ligase (LIG I).
Sinha
Krishna Murari
KM
Molecular Biology Institute and Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Hines
Jane C
JC
Downey
Nicholas
N
Ray
Dan S
DS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016831:Crithidia fasciculata / Q000201:enzymology / Q000235:genetics*; D011088:DNA Ligases / Q000378:metabolism*; D017931:DNA Primers; D004250:DNA Topoisomerases, Type II / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics*; D008969:Molecular Sequence Data; D014345:Trypanosoma / Q000201:enzymology / Q000235:genetics*
D017931:DNA Primers; D004272:DNA, Mitochondrial; D016054:DNA, Protozoan; D004250:DNA Topoisomerases, Type II; D011088:DNA Ligases