title: Total internal reflection-based biochip utilizing a polymer-filled cavity with a micromirror sidewall.
abstract: A total internal reflection (TIR)-based biochip utilizing a polymer-filled cavity with a micromirror sidewall has been designed and fabricated. The implementation of the micromirror sidewall cavity facilitates precise alignment of the excitation light beam into the system. The incident angle of illumination can be easily modified by selecting polymers of different indices of refraction while optical losses are minimized. The design enables the hybrid, vertical integration of a laser diode and a CCD camera, resulting in a compact optical system. Brownian motion of fluorescent microspheres and real-time photobleaching of rhodamine 6G molecules is demonstrated. The proposed TIR-based chip simplifies current TIR optical configurations and could potentially be used as an optical-microfluidic platform for an integrated lab-on-a-chip microsystem.
authors.affiliation: Berkeley Sensor and Actuator Center, Department of Bioengineering, University of California, Berkeley, USA.
authors.forename: Nikolas
authors.initials: N
authors.lastname: Chronis
authors.forename: Luke P
authors.initials: LP
authors.lastname: Lee
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004867:Equipment Design; D044085:Microfluidics / Q000295:instrumentation; D008856:Microscopy, Fluorescence / Q000295:instrumentation; D055095:Optics and Photonics / Q000295:instrumentation*; D038761:Photobleaching; D011108:Polymers / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D011108:Polymers; D012235:Rhodamines