Phaiodactylipin, a glycosylated heterodimeric phospholipase A from the venom of the scorpion Anuroctonus phaiodactylus.
Phaiodactylipin was purified from the venom of the scorpion Anuroctonus phaiodactylus. It is the first protein to be purified from a scorpion of the family Iuridae and has a molecular mass of 19 172 atomic mass units. The mature protein is composed of two subunits, the large one consisting of 108 amino acid residues, whereas the small subunit has only 18 residues, and the structure is stabilized by five disulfide bridges. The heterodimer is expressed from a single message containing 769 base pairs and a signal peptide with 16 and/or 25 amino acid residues. During maturation an internal hexapeptide is excised. There are three putative sites of N-glycosylation, one of which is situated in the small subunit region. The carbohydrate composition of this site was determined by mass spectrometry analysis and was found to contain three hexoses, two N-acetyl-hexoses and two deoxyhexoses. The protein has a calcium dependent phospholipase A(2) type of activity. It is lethal to arthropods (insects and isopods), but not toxic to mammals, using doses up to 20 microg per 20 g mouse body weight. For crickets, a dose of 5 microg per animal is lethal; however, when injected into mice it is capable of causing only muscular inflammation, without rupture of the basal membrane of cells. It has a direct hemolytic effect in human erythrocytes and retards the coagulation time of blood. It is an unusual phospholipase A(2), with only 36% and 50% amino acid sequence identities to the closest known phospholipases, imperatoxin I and phospholipin, respectively. Identities with bee and Heloderma venom phospholipase are only in the order of 28%.
Valdez-Cruz
Norma A
NA
Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology, National Autonomous University of Mexico, Avenida Universidad 2001, PO Box 510-3, Cuernavaca 62210, Mexico.
Batista
Cesar V F
CV
Possani
Lourival D
LD
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D002241:Carbohydrates / Q000032:analysis / Q000737:chemistry; D018076:DNA, Complementary / Q000235:genetics; D019281:Dimerization; D004912:Erythrocytes / Q000187:drug effects; D006031:Glycosylation; D006461:Hemolysis / Q000187:drug effects; D006614:Hindlimb; D006801:Humans; D007249:Inflammation / Q000139:chemically induced; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D010741:Phospholipases A / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D010743:Phospholipids / Q000378:metabolism; D010802:Phylogeny; D012604:Scorpion Venoms / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
D002241:Carbohydrates; D018076:DNA, Complementary; D010446:Peptide Fragments; D010743:Phospholipids; D012604:Scorpion Venoms; D010741:Phospholipases A; D002118:Calcium