title: Global gene expression analysis of rat colon cancers induced by a food-borne carcinogen, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine.
abstract: Colon cancers develop after accumulation of multiple genetic and epigenetic alterations in colon epithelial cells. To shed light on global changes in gene expression of colon cancers and to gain further insight into the molecular mechanisms underlying colon carcinogenesis, we have conducted a comprehensive microarray analysis of mRNA using a rat colon cancer model with the food-borne carcinogen, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). Of 8749 genes or ESTs on a high density oligonucleotide microarray, 27 and 46 were over- and underexpressed, respectively, by > or =3-fold in colon cancers in common in two rat strains with distinct susceptibility to PhIP carcinogenesis. For example, genes involved in inflammation and matrix proteases and a cell cycle regulator gene, cyclin D2, were highly expressed in colon cancers. In contrast, genes encoding structural proteins, muscle-related proteins, matrix-composing and mucin-like proteins were underexpressed. Interestingly, a subset of genes whose expression is characteristic of Paneth cells, i.e. the defensins and matrilysin, were highly overexpressed in colon cancers. The presence of defensin 3 and defensin 5 transcripts in cancer cells could also be confirmed by in situ mRNA hybridization. Furthermore, Alcian blue/periodic acid Schiff base (AB-PAS) staining and immunohistochemical analysis with an anti-lysozyme antibody demonstrated Paneth cells in the cancer tissues. AB-PAS-positive cells were also observed in high grade dysplastic aberrant crypt foci, which are considered to be preneoplastic lesions of the colon. Our results suggest that Paneth cell differentiation in colon epithelial cells could be an early morphological change in cryptic cells during colon carcinogenesis.
authors.affiliation: Biochemistry Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Fujiwara
authors.forename: Masako
authors.initials: M
authors.lastname: Ochiai
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Ohta
authors.forename: Misao
authors.initials: M
authors.lastname: Ohki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Aburatani
authors.forename: Minako
authors.initials: M
authors.lastname: Nagao
authors.forename: Takashi
authors.initials: T
authors.lastname: Sugimura
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nakagama
mesh_terms: D000423:Alcian Blue / Q000494:pharmacology; D000818:Animals; D002273:Carcinogens; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000378:metabolism*; D056742:Cyclin D2; D016213:Cyclins / Q000378:metabolism; D023082:Defensins / Q000378:metabolism; D020224:Expressed Sequence Tags; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007093:Imidazoles; D007150:Immunohistochemistry; D009113:Muramidase / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D009841:Oligonucleotides / Q000737:chemistry; D019879:Paneth Cells / Q000166:cytology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C080336:CCND2 protein, human; D002273:Carcinogens; C486197:Ccnd2 protein, rat; D056742:Cyclin D2; D016213:Cyclins; D023082:Defensins; D007093:Imidazoles; D009841:Oligonucleotides; D012333:RNA, Messenger; C049584:2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine; D009113:Muramidase; D000423:Alcian Blue