title: Performance of a newly developed integrant of Zymomonas mobilis for ethanol production on corn stover hydrolysate.
abstract: Efficient conversion of lignocellulosic biomass requires biocatalysts able to tolerate inhibitors produced by many pretreatment processes. Recombinant Zymomonas mobilis 8b, a recently developed integrant of Zymomonas mobilis 31821(pZB5), tolerated acetic acid up to 16 g l(-1) and achieved 82%-87% (w/w) ethanol yields from pure glucose/xylose solutions at pH 6 and temperatures of 30 degrees C and 37 degrees C. An ethanol yield of 85% (w/w) was achieved on glucose/xylose from hydrolysate produced by dilute sulfuric acid pretreatment of corn stover after an overliming' process was used to improve hydrolysate fermentability.
authors.affiliation: National Bioenergy Center National Renewable Energy Laboratory, Golden, CO 80401, USA. ali_mohagheghi@nrel.gov
authors.forename: Ali
authors.initials: A
authors.lastname: Mohagheghi
authors.forename: Nancy
authors.initials: N
authors.lastname: Dowe
authors.forename: Daniel
authors.initials: D
authors.lastname: Schell
authors.forename: Yat-Chen
authors.initials: YC
authors.lastname: Chou
authors.forename: Christina
authors.initials: C
authors.lastname: Eddy
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000085:Acetates / Q000737:chemistry; D019342:Acetic Acid / Q000737:chemistry; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002482:Cellulose / Q000737:chemistry; D003470:Culture Media / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008031:Lignin / Q000737:chemistry; D011767:Pyruvate Decarboxylase / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D013464:Sulfuric Acids / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors; D014994:Xylose / Q000378:metabolism; D003313:Zea mays / Q000378:metabolism*; D017355:Zymomonas / Q000378:metabolism*
chemical_list: D000085:Acetates; D003470:Culture Media; D011994:Recombinant Proteins; D013464:Sulfuric Acids; C036909:lignocellulose; D000431:Ethanol; D002482:Cellulose; D008031:Lignin; D014994:Xylose; D011767:Pyruvate Decarboxylase; D005947:Glucose; C033158:sulfuric acid; D019342:Acetic Acid