title: [Genomic typing techniques in a case of skin infection epidemic by Staphylococcus aureus in a newborn unit].
abstract: In the present study it has been attempted to characterize the source, the reservoir and the modality of transmission of a skin infection epidemic by Staphylococcus aureus in a Newborn Unit. Every S. aureus isolate from clinician and environmental samples has been submitted to phenotypic typing, by means of antibiotic resistance patterns. The genotype has been analyzed using genomic DNA profile of restriction with Sma I and Pulsed Field Gel Electrophoresis (PFGE). In bacterial infections the epidemiologist nexus can think demonstrated when the stocks isolate from various cases belong to the same one clone. In the Newborn Unit the same one clone of S. aureus has been found on 2 health care workers, on 13 staff bearers characterized, and skin of 5 ill newborn infants. Therefore it has been able to go back to the probable source of infection and to take the right measures.
authors.affiliation: Dipartimento di Scienze Mediche Preventive, Facoltà di Medicina e Chirurgia, Università degli Studi di Napoli Federico II. deriu@unina.it
authors.forename: I
authors.initials: I
authors.lastname: Torre
authors.forename: F P
authors.initials: FP
authors.lastname: Sferratore
authors.forename: M
authors.initials: M
authors.lastname: Crispino
mesh_terms: D015373:Bacterial Typing Techniques; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission; D004269:DNA, Bacterial / Q000032:analysis; D004196:Disease Outbreaks; D005838:Genotype; D006801:Humans; D007231:Infant, Newborn; D009725:Nurseries, Hospital; D013207:Staphylococcal Skin Infections / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics
chemical_list: D004269:DNA, Bacterial