title: Using a non-invasive stable isotope tracer to measure the absorption of water in humans.
abstract: The development of solutions that prevent dehydration or promote adequate re-hydration play a vital role in preventing fatigue during exercise, however, the methods commonly used to assess the hydration ability of such solutions are invasive and often assess the components of absorption separately. This paper describes using a non-invasive deuterium tracer technique that assesses gastric emptying and intestinal absorption simultaneously to evaluate the uptake of water during rest and exercise. The kinetics of absorption are further examined by mathematical modelling of the data generated. For the rest group, 0.05 g/kg of body weight of deuterium, contained in gelatine capsules, was ingested with ordinary tap water and saliva samples were collected every 5 min for one hour while the subject remained seated. The deuterium was administered as above for the exercise group but sample collection was during one hour of exercise on a treadmill at 55% of the subject's maximum heart rate. The enrichment data for each subject were mathematically modelled and the parameters obtained were compared across groups using an independent samples t-test. Compared with the rest condition, the exercise group showed delayed absorption of water as indicated by significant differences for the modelling parameters t2, t1/2, maximum absorption rate and solution absorption amount at t1. Labelling with a deuterium tracer is a good measure of the relative rate ingested fluids are absorbed by the body. Mathematical modelling of the data generates rates of maximum absorption and allows calculation of the percentage of the solution that is absorbed at any given time during the testing period.
authors.affiliation: Children's Nutrition Research Centre, Department of Paediatrics and Child Health, University of Queensland, Royal Children's Hospital, Herston, Queensland 4029, Australia. rj.hill@mailbox.uq.edu.au
authors.forename: Rebecca J
authors.initials: RJ
authors.lastname: Hill
authors.forename: Leslie J C
authors.initials: LJ
authors.lastname: Bluck
authors.forename: Peter S W
authors.initials: PS
authors.lastname: Davies
mesh_terms: D000293:Adolescent; D000328:Adult; D001826:Body Fluids / Q000737:chemistry; D003903:Deuterium / Q000032:analysis; D015444:Exercise; D005080:Exercise Test; D005260:Female; D005746:Gastric Emptying; D006801:Humans; D007408:Intestinal Absorption / Q000502:physiology*; D008297:Male; D008954:Models, Biological; D014867:Water / Q000378:metabolism*
chemical_list: D014867:Water; D003903:Deuterium