Efficacy of early systemic betamethasone or cyclosporin A after corneal alkali injury via inflammatory cytokine reduction.
OBJECTIVE
To evaluate the effects of early systemic administration of betamethasone or cyclosporin A (CsA) on inflammatory cytokine expression and corneal damage after alkali injury.


METHODS
Sixty-five Wistar rat corneas injured with 1N NaOH were divided into three groups: untreated, betamethasone-treated and CsA-treated. Both agents were administered systemically and daily during the first 7 days after injury. Interleukin (IL)-1alpha, -6 and -8 concentrations in the injured corneas were measured with ELISA at 2, 4 and 7 days after injury. Corneal damage was evaluated by scoring clinical findings.


RESULTS
In untreated injured corneas, IL-1alpha, -6 and -8 were markedly elevated during the 7-day period following injury. Both betamethasone and CsA significantly suppressed IL-1alpha and IL-8 at day 4. Only betamethasone significantly suppressed IL-6 at days 4 and 7. Both agents significantly reduced corneal opacity at day 4.


CONCLUSIONS
Early systemic administration of betamethasone or CsA after alkali injury may be of benefit by suppressing inflammatory cytokine expression in the cornea.
Den
Seika
S
Department of Ophthalmology, Osaka Medical College, Takatsuki, Osaka, Japan. seika@yacht.ocn.ne.jp
Sotozono
Chie
C
Kinoshita
Shigeru
S
Ikeda
Tsunehiko
T
D000818:Animals; D001623:Betamethasone / Q000008:administration & dosage*; D002057:Burns, Chemical / Q000378:metabolism*; D003315:Cornea / Q000378:metabolism*; D016572:Cyclosporine / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005126:Eye Burns / Q000139:chemically induced*; D005260:Female; D005938:Glucocorticoids / Q000008:administration & dosage; D007166:Immunosuppressive Agents / Q000008:administration & dosage; D007273:Injections, Intramuscular; D007279:Injections, Subcutaneous; D007378:Interleukins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012972:Sodium Hydroxide / Q000633:toxicity
D005938:Glucocorticoids; D007166:Immunosuppressive Agents; D007378:Interleukins; D012972:Sodium Hydroxide; D016572:Cyclosporine; D001623:Betamethasone