Studies on immobilized lipase in hydrophobic sol-gel.
The hydrolysis of tetraethoxysilane using the sol-gel process was used to produce silica matrices, and these were tested for the immobilization of lipase from Candida rugosa by three methods: physical adsorption, covalent binding, and gel entrapment in the presence and absence of polyethylene glycol (PEG-1450). The silica matrices and their derivatives were characterized regarding particle size distribution, specific surface area, pore size distribution (Brunauer, Emmett, and Teller [B.E.T.] method), yield of grafting (thermogravimetric analyzer [TGA]), and chemical composition (Fourier transform infrared). Immobilization yields based on recovered lipase activity varied from 3.0 to 32.0%, and the highest efficiency was attained when lipase was encapsulated in the presence of PEG.
Soares
Cleide M F
CM
State University of Maringa, Department of Chemical Engineering, Av. Colombo 5790, Bloco D-90, 87020-900, Maringa-PR, Brazil.
dos Santos
Onelia A
OA
de Castro
Heizir F
HF
de Moraes
Flavio F
FF
Zanin
Gisella M
GM
D000327:Adsorption; D001709:Biotechnology / Q000379:methods; D002175:Candida / Q000201:enzymology; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006868:Hydrolysis; D008049:Lipase / Q000737:chemistry*; D044367:Phase Transition; D011092:Polyethylene Glycols / Q000737:chemistry; D012821:Silanes / Q000737:chemistry; D012825:Silicon / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
D004800:Enzymes, Immobilized; D012821:Silanes; D011092:Polyethylene Glycols; C040733:tetraethoxysilane; D008049:Lipase; D012825:Silicon