title: ESF1 is required for 18S rRNA synthesis in Saccharomyces cerevisiae.
abstract: We report that Esf1p (Ydr365cp), an essential, evolutionarily conserved nucleolar protein, is required for the biogenesis of 18S rRNA in Saccharomyces cerevisiae. Depletion of Esf1p resulted in delayed processing of 35S precursor and a striking loss of 18S rRNA. Esf1p physically associated with ribosomal proteins and proteins involved in 18S rRNA biogenesis. Consistent with its role in 18S rRNA biogenesis, Esf1p also physically associated with U3 and U14 snoRNAs, but did not appear to be a core component of the SSU processome. These data indicate that Esf1p plays a direct role in early pre-rRNA processing.
authors.affiliation: Banting and Best Department of Medical Research, University of Toronto, 112 College Street, Toronto, Ontario M5G 1L6, Canada.
authors.forename: Wen-Tao
authors.initials: WT
authors.lastname: Peng
authors.forename: Nevan J
authors.initials: NJ
authors.lastname: Krogan
authors.forename: Dawn P
authors.initials: DP
authors.lastname: Richards
authors.forename: Jack F
authors.initials: JF
authors.lastname: Greenblatt
authors.forename: Timothy R
authors.initials: TR
authors.lastname: Hughes
mesh_terms: D000595:Amino Acid Sequence; D017124:Conserved Sequence; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology*; D012322:RNA Precursors / Q000737:chemistry / Q000378:metabolism; D012337:RNA, Ribosomal, 18S / Q000096:biosynthesis* / Q000737:chemistry; D020537:RNA, Small Nucleolar / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology*; D016415:Sequence Alignment
chemical_list: C487887:ESF1 protein, S cerevisiae; D009687:Nuclear Proteins; D012322:RNA Precursors; D012337:RNA, Ribosomal, 18S; D020537:RNA, Small Nucleolar; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins