[Investigation of molecular-genetic heterogeneity of clematis plants (Clematis L.) obtained by organogenesis and somatic embryogenesis in vitro].
Genome variability of in vitro micropropagated Clematis plants was established. The optimum concentrations of BAP and zeatin in the culture medium regulating in vitro morphogenetic processes in clematis explants cv. Serenada Kryma were determined. Molecular-genetic analysis of Clematis plants obtained via in vitro somatic embryogenesis and organogenesis was carried out. Using ISSR primers 105 amplicons have been revealed, six of them were polymorphic. The mean index of heterogeneity of clematis plants was 5.7%.
Mitrofanova
I V
IV
Galaev
A V
AV
Sivolap
Iu M
IuM
D039324:Clematis / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D018744:DNA, Plant / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000196:embryology / Q000502:physiology*; D018521:Plant Physiological Phenomena; D018520:Plant Shoots / Q000187:drug effects / Q000196:embryology / Q000502:physiology*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012038:Regeneration / Q000187:drug effects; D012639:Seeds / Q000187:drug effects / Q000196:embryology / Q000502:physiology*
D018744:DNA, Plant; D010937:Plant Growth Regulators