Isolation and characterization of cerebrosides of the hydrocarbon-assimilating yeast Yarrowia lipolytica.
Yarrowia lipolytica yeast was grown batchwise on n-hexadecane as the carbon and energy source. Two cerebroside species were quantitatively isolated from sphingolipid fractions of total lipids by a combination of column chromatography and preparative high-performance thin-layer chromatography. The cerebroside content accounted for 1.3% of the total cell lipids. Glucose was detected as the sole sugar constituent in cerebrosides. The fatty acid composition of cerebrosides was characterized by a high proportion of hydroxylated long-chain saturated fatty acids. The major fatty acids were h16:0 and 16:0. The long-chain bases composition shows a preponderance of trihydroxy bases and a small amount of dihydroxy bases. The striking finding was a high proportion of 19-phytosphingosine.
Rupcić
Jasminka
J
Department of Chemistry and Biochemistry, Medical Faculty, University of Rijeka, B. Branchetta 20, HR-51000 Rijeka, Croatia. rjasmin@medri.hr
Georgiù
Kata
K
Marić
Vladimir
V
D000473:Alkanes; D002554:Cerebrosides / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D002855:Chromatography, Thin Layer; D003470:Culture Media; D005227:Fatty Acids / Q000032:analysis; D005947:Glucose; D006601:Hexoses / Q000737:chemistry; D025062:Yarrowia / Q000254:growth & development / Q000378:metabolism*
D000473:Alkanes; D002554:Cerebrosides; D003470:Culture Media; D005227:Fatty Acids; D006601:Hexoses; C007932:n-hexadecane; D005947:Glucose