title: Immunolocalization of CXC chemokine and recruitment of polymorphonuclear leukocytes in the rat molar periodontal tissue after topical application of lipopolysaccharide.
abstract: This study investigated the recruitment of polymorphonuclear leukocytes (PMNs) and the immunolocalization of CXC chemokines, including macrophage inflammatory protein-2 (MIP-2) and cytokine-induced neutrophil chemoattractant-2 (CINC-2) in rat periodontal tissue after topical application of lipopolysaccharide (LPS; 5 mg/ml) from Escherichia coli into the rat molar gingival sulcus. In normal periodontal tissues, a small number of MIP-2- and CINC-2-positive cells were seen in junctional epithelium (JE), especially in its coronal half. After topical application of LPS, a prominent increase of MIP-2- and CINC-2-positive JE cells was observed. Almost all JE cells strongly expressed them at day 1 and day 2, and then the number of chemokine-positive cells returned to normal at day 7. Corresponding to these chemokine expressions, LPS application induced a significant increase in the number of PMNs in the sub-JE area from 1 h to 2 days and a significant increase in JE area from 3 h to 5 days, indicating a dynamic flow of PMNs from the sub-JE area into JE. These findings indicated that JE cells produced MIP-2 and CINC-2 in response to LPS stimulation and suggested that MIP-2 and CINC-2 may be responsible for PMN migration toward the periodontal pathogen and may play an important role in the initiation of inflammation and subsequent periodontal tissue destruction.
authors.affiliation: Department of Oral Maxillofacial Pathobiology, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, 734-8553, Hiroshima, Japan.
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Miyauchi
authors.forename: Shoji
authors.initials: S
authors.lastname: Kitagawa
authors.forename: Masae
authors.initials: M
authors.lastname: Hiraoka
authors.forename: Akihisa
authors.initials: A
authors.lastname: Saito
authors.forename: Sunao
authors.initials: S
authors.lastname: Sato
authors.forename: Yasusei
authors.initials: Y
authors.lastname: Kudo
authors.forename: Ikuko
authors.initials: I
authors.lastname: Ogawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Takata
mesh_terms: D000287:Administration, Topical; D000818:Animals; D054426:Chemokine CXCL2; D019743:Chemokines, CXC / Q000032:analysis / Q000378:metabolism* / Q000502:physiology; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis / Q000502:physiology; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D008963:Molar / Q000276:immunology / Q000473:pathology*; D015846:Monokines / Q000032:analysis / Q000502:physiology; D009504:Neutrophils / Q000502:physiology*; D010518:Periodontitis / Q000276:immunology / Q000473:pathology; D010519:Periodontium / Q000276:immunology / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D054426:Chemokine CXCL2; D019743:Chemokines, CXC; C513296:Cxcl2 protein, rat; C513294:Cxcl3 protein, rat; D036341:Intercellular Signaling Peptides and Proteins; D008070:Lipopolysaccharides; D015846:Monokines