Strategies for covalent attachment of DNA to beads.
Several covalent attachment chemistries were tested for the immobilization of DNA onto glass beads. The comparison was based on the ability of these chemistries to produce derivatized beads that give good hybridization signals. Cyanuric chloride, isothiocyanate, nitrophenyl chloroformate, and hydrazone chemistries gave us the best (yet comparable) hybridization signals. We further characterized the cyanuric chloride method for the number of attachment sites, number of hybridizable sites, hybridization kinetics, effect of linker length on hybridization intensity and stability of the derivatized beads.
Steinberg
Gali
G
Illumina, Inc., 9885 Towne Centre Drive, San Diego, CA 92121, USA.
Stromsborg
Katie
K
Thomas
Lynette
L
Barker
David
D
Zhao
Chanfeng
C
D003432:Cross-Linking Reagents; D004247:DNA / Q000737:chemistry*; D005898:Glass; D008863:Microspheres; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012821:Silanes / Q000737:chemistry
D003432:Cross-Linking Reagents; D012821:Silanes; D004247:DNA