title: Response of seizure-induced newborn neurons in the dentate gyrus of adult rats to second episode of seizures.
abstract: In this study we examined the unknown issue of whether seizure-induced newborn hippocampal neurons in freely moving adult rats are able to respond to pathophysiological stimuli in the same way as their neighboring neurons do. Three days after pentylenetrazol (PTZ)-induced generalized seizures, rats received 5-bromodeoxyuridine (BrdU) injections to label dividing cells, followed 4 weeks later by the second PTZ injection to induce second episode of generalized seizures. We observed that the first episode of PTZ-induced seizures resulted in a significant increase in the number of newborn neurons in the adult hippocampal dentate gyrus. In comparison with vehicle-injected control rats that exhibited no Fos immunoreactivity and mild glutamic acid decarboxylase 67 (GAD67) expression in the dentate granule cells, rats killed 2-6 h following the second PTZ injection showed intensive Fos and GAD67 expression in virtually all granule cells with or without BrdU double-labeling. These findings provide important evidence indicating that seizure-induced newborn neurons in freely moving adult rats are able to respond to pathophysiological stimuli in the same way as neighboring neurons do.
authors.affiliation: Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. wen.jiang@usask.ca
authors.forename: Wen
authors.initials: W
authors.lastname: Jiang
authors.forename: Jin-Cun
authors.initials: JC
authors.lastname: Wang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhang
authors.forename: Aaron H
authors.initials: AH
authors.lastname: Sheerin
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001522:Behavior, Animal; D001973:Bromodeoxyuridine / Q000378:metabolism; D018891:Dentate Gyrus / Q000254:growth & development / Q000473:pathology*; D004334:Drug Administration Schedule; D005968:Glutamate Decarboxylase / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D016761:Oncogene Proteins v-fos / Q000378:metabolism; D010433:Pentylenetetrazole; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors
chemical_list: D007527:Isoenzymes; D016761:Oncogene Proteins v-fos; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1; D001973:Bromodeoxyuridine; D010433:Pentylenetetrazole