The effect of Ginkgo extract EGb761 in cisplatin-induced peripheral neuropathy in mice.
Neuroprotective effect of Ginkgo biloba extract EGb761 in cisplatin (cis-diamminedi-chloroplatinum, or CDDP)-induced peripheral neuropathy was investigated. Swiss albino mice were treated with CDDP, 2 mg/kg ip twice a week for nine times. One group of the animals also received EGb761 in the drinking water at an estimated dosage of 100 mg/kg per day. Two other groups received vehicle (control) or EGb761 only. Development of neuropathy was evaluated with changes in sensory nerve conduction velocity (NCV). Following the treatments, dorsal root ganglia (DRGs) were microscopically examined and some were cultured for 3 days. EGb761 proved effective in preventing the reduction in NCV (P < 0.0001) caused by CDDP. CDDP caused a decrease in the number of migrating cells (P < 0.01) and in the length of outgrowing axons (P < 0.01) while EGb761 treatment prevented the latter. CDDP led to smaller nuclear and somatic sizes in neurons (P < 0.01), while with EGb761 co-administration, both were close to control values. Animals having EGb761 only had similar results with controls. In conclusion, EGb761 was found to be effective in preventing some functional and morphological deteriorations in CDDP-induced peripheral neuropathy.
Oztürk
Gürkan
G
Department of Physiology, Neuroscience Research Unit, Medical School, Yüzüncü Yil University, Van, Turkey. drgurkan@yyu.edu.tr
Anlar
Omer
O
Erdoğan
Ender
E
Kösem
Mustafa
M
Ozbek
Hanefi
H
Türker
Aybars
A
D000284:Administration, Oral; D000818:Animals; D001369:Axons / Q000187:drug effects / Q000473:pathology; D002945:Cisplatin / Q000633:toxicity*; D004195:Disease Models, Animal; D004558:Electric Stimulation; D004566:Electrodes; D005260:Female; D005727:Ganglia, Spinal / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D020441:Ginkgo biloba; D007274:Injections, Intraperitoneal; D051379:Mice; D008856:Microscopy, Fluorescence; D009431:Neural Conduction / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010523:Peripheral Nervous System Diseases / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D010936:Plant Extracts / Q000494:pharmacology*; D000096983:Ginkgo Extract
D002945:Cisplatin; D018696:Neuroprotective Agents; D010936:Plant Extracts; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract