The nitration of tau protein in neurone-like PC12 cells.
Tyrosine nitration of proteins is emerging as a post-translational modification playing a role in physiological conditions. Looking for the molecular events triggered by nitric oxide in nerve growth factor-induced neuronal differentiation, we now find that nitration occurs on the microtubule-associated protein tau. In differentiated PC12 cells, we have identified as tau a nitrated protein that co-immunoprecipitates with alpha-tubulin and indicated that the modified protein is associated with the cytoskeleton but it is confined to a restricted cell region. This paper supplies the first evidence that nitration of tau occurs in a physiological process and suggests that it could play a role in neuronal differentiation.
Cappelletti
Graziella
G
Department of Biology, University of Milan, Via Celoria 26, 20133 Milan, Italy. graziella.cappelletti@unimi.it
Tedeschi
Gabriella
G
Maggioni
Maria G
MG
Negri
Armando
A
Nonnis
Simona
S
Maci
Rosalba
R
D000595:Amino Acid Sequence; D000818:Animals; D003599:Cytoskeleton / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D016716:PC12 Cells; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism; D016875:tau Proteins / Q000737:chemistry / Q000378:metabolism*
D010455:Peptides; D016875:tau Proteins; D009569:Nitric Oxide; C002744:3-nitrotyrosine; D014443:Tyrosine