title: [Primary studies on synthesis and properties of mitoxantrone-insulin conjugates].
abstract: OBJECTIVE
  Mitoxantrone-insulin conjugate was prepared through covalent bond, where mitoxantrone was an anticancer model drug and insulin acted as a vector. Its stability in vitro was discussed.
  
  
  METHODS
  Spacer was used to join insulin and mitoxantrone. The intermediate and the final conjugate were characterized by infra-red spectroscopy (IR), 1H NMR, mass spectrometray (MS) and ultra-violet spectrophotometry (UV). Stability trials of the conjugate were carried out in different pH buffer solutions and mouse plasma at 37 degrees C.
  
  
  RESULTS
  Drug loading of the conjugate was 11.68%. The conjugate was stable in phosphate buffer solution of pH 2-8 and mouse plasma at 37 degrees C.
  
  
  CONCLUSIONS
  Stable mitoxantrone-insulin conjugate was obtained to ensure further tumor targeted study in vivo.
authors.affiliation: Department of Pharmaceatics, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
authors.forename: Wen-sheng
authors.initials: WS
authors.lastname: Liu
authors.forename: Zhi-rong
authors.initials: ZR
authors.lastname: Zhang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage / Q000737:chemistry*; D004337:Drug Carriers; D016503:Drug Delivery Systems; D004355:Drug Stability; D007328:Insulin / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008942:Mitoxantrone / Q000008:administration & dosage / Q000737:chemistry*; D013055:Spectrophotometry, Infrared
chemical_list: D000964:Antimetabolites, Antineoplastic; D004337:Drug Carriers; D007328:Insulin; D008942:Mitoxantrone