Human hepatic cell uptake of resveratrol: involvement of both passive diffusion and carrier-mediated process.
This work reports significant advances on the transport in hepatic cells of resveratrol, a natural polyphenol with potent protective properties. First, we describe a new simple technique to qualitatively follow resveratrol cell uptake and intracellular distribution, based on resveratrol fluorescent properties. Second, the time-course study and the quantification of (3)H-labelled resveratrol uptake have been performed using human hepatic derived cells (HepG2 tumor cells) and hepatocytes. The temperature-dependence of the kinetics of uptake as well as the cis-inhibition experiments agree with the involvement of a carrier-mediated transport in addition to passive diffusion. The decrease of passive uptake resulting from resveratrol binding to serum proteins brings to light a mediated mechanism in physiological situation.
Lançon
A
A
Laboratoire de Biologie Moléculaire et Cellulaire, Université de Bourgogne, 6 boulevard Gabriel, 21000 Dijon, France.
Delmas
D
D
Osman
H
H
Thénot
J-P
JP
Jannin
B
B
Latruffe
N
N
D001682:Biological Availability; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D004058:Diffusion; D018197:Hepatoblastoma / Q000378:metabolism*; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D008657:Metabolic Clearance Rate; D000077185:Resveratrol; D013267:Stilbenes / Q000493:pharmacokinetics*; D013696:Temperature; D014018:Tissue Distribution
D002352:Carrier Proteins; D013267:Stilbenes; D000077185:Resveratrol