Biologically active antisense phosphorothioate oligodeoxyribonucleotides: synthesis, characterization, and studies of 3'-terminal phosphorothioate monoester analogues.
Multiple phosphorothioate oligonucleotides containing a 3'-terminal negative charge were synthesized and characterized. Influence of the added negative charge on activation of duplexes by RNase H was investigated. No additional help in recruitment of RNase H was observed.
Ravikumar
Vasulinga T
VT
Isis Pharmaceuticals, Carlsbad, California 92008, USA. vravikumar@isisph.com
Lima
Walt F
WF
Van Sooy
Kent
K
Turney
Brett
B
D020319:Oligodeoxyribonucleotides, Antisense / Q000138:chemical synthesis* / Q000737:chemistry
D020319:Oligodeoxyribonucleotides, Antisense