title: The external promoter in the guinea pig 5S rRNA gene is different from the rodent promoter.
abstract: The guinea pig has about 100 copies of the 5S rRNA gene per haploid genome and they are present in 2.1 kb tandem repeats. Three bona fide 5S rRNA genes and four pseudo genes were sequenced. The conserved external promoter (D box) found in rodents and primates is only partially present in the guinea pig. The "D box like" sequence in guinea pig only has eight of the 12 nucleotides in the conserved D box. The results are in accordance with investigations showing that the guinea pig is not a rodent. Conserved sequences in the non-transcribed spacer can therefore be useful in phylogenetic studies.
authors.affiliation: Department of Medical Biochemistry and Genetics, Biochemistry Laboratory B, The Panum Institute, University of Copenhagen, Copenhagen, Denmark. sune@imbg.ku.dk
authors.forename: Sune
authors.initials: S
authors.lastname: Frederiksen
authors.forename: Jesper
authors.initials: J
authors.lastname: Heeno Andersen
mesh_terms: D000818:Animals; D001483:Base Sequence; D006168:Guinea Pigs; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D012377:Rodentia / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D012341:RNA, Ribosomal, 5S