Characterization of Cfa1, a monofunctional acyl carrier protein involved in the biosynthesis of the phytotoxin coronatine.
Cfa1 was overproduced in Escherichia coli and Pseudomonas syringae, and the degree of 4'-phosphopantetheinylation was determined. The malonyl-coenzyme A:acyl carrier protein transacylase (FabD) of P. syringae was overproduced and shown to catalyze malonylation of Cfa1, suggesting that FabD plays a role in coronatine biosynthesis. Highly purified Cfa1 did not exhibit self-malonylation activity.
Seidle
Heather
H
Department of Chemistry, Rice University, Houston, Texas 77005, USA.
Rangaswamy
Vidhya
V
Couch
Robin
R
Bender
Carol L
CL
Parry
Ronald J
RJ
D000213:Acyl Carrier Protein / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D051080:Acyl-Carrier Protein S-Malonyltransferase; D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D000596:Amino Acids / Q000096:biosynthesis*; D001427:Bacterial Toxins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins; D054889:Fatty Acid Synthase, Type II; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism*; D007192:Indenes / Q000032:analysis / Q000378:metabolism*; D008969:Molecular Sequence Data; D044224:Pseudomonas syringae / Q000378:metabolism
D000213:Acyl Carrier Protein; D000596:Amino Acids; D001427:Bacterial Toxins; D029968:Escherichia coli Proteins; D007192:Indenes; C022924:colonization factor antigens; D036781:Fimbriae Proteins; C058487:coronatine; C084266:coronafacic acid; D000217:Acyltransferases; D051080:Acyl-Carrier Protein S-Malonyltransferase; C494968:fabD protein, E coli; D054889:Fatty Acid Synthase, Type II