title: Conservation of a proteinase cleavage site between an insect retrovirus (gypsy) Env protein and a baculovirus envelope fusion protein.
abstract: The predicted Env protein of insect retroviruses (errantiviruses) is related to the envelope fusion protein of a major division of the Baculoviridae. The highest degree of homology is found in a region that contains a furin cleavage site in the baculovirus proteins and an adjacent sequence that has the properties of a fusion peptide. In this investigation, the homologous region in the Env protein of the gypsy retrovirus of Drosophila melanogaster (DmegypV) was investigated. Alteration of the predicted DmegypV Env proteinase cleavage site from RIAR to AIAR significantly reduced cleavage of Env in both Spodoptera frugiperda (Sf-9) and D. melanogaster (S2) cell lines. When the predicted DmegypV Env cleavage site RIAR was substituted for the cleavage sequence RRKR in the Lymantria dispar nucleopolyhedrovirus fusion protein (LD130) sequence, cleavage of the hybrid LD130 molecules still occurred, although at a reduced level. The conserved 21-amino acid sequence just downstream of the cleavage site, which is thought to be the fusion peptide in LD130, was also characterized. When this sequence from DmegypV Env was substituted for the homologous sequence in LD130, cleavage still occurred, but no fusion was observed in either cell type. In addition, although a DmegypV-Env-green fluorescent protein construct localized to cell membranes, no cell fusion was observed.
authors.affiliation: Department of Microbiology, Oregon State University, Corvallis, OR 97331-3804, USA.
authors.forename: Margot N
authors.initials: MN
authors.lastname: Pearson
authors.forename: George F
authors.initials: GF
authors.lastname: Rohrmann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000737:chemistry / Q000235:genetics*; D001665:Binding Sites; D002459:Cell Fusion; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D004331:Drosophila melanogaster; D010450:Endopeptidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015686:Gene Products, env / Q000737:chemistry / Q000235:genetics*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009154:Mutation; D012190:Retroviridae / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D014162:Transfection; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D015686:Gene Products, env; D014760:Viral Fusion Proteins; D010450:Endopeptidases