Determination of plasma amino acids by fluorescent derivatization and reversed-phase liquid chromatographic separation.
Amino acid analysis in physiological fluids remains expensive, as it usually requires a dedicated analyzer. We modified an RP-HPLC method originally devoted to peptide and protein hydrolysate analysis in order to apply it to plasma amino acid determination. The described method uses a commercial kit based on 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate derivatization followed by reversed-phase high-performance liquid chromatography. The described method is easy to use and allows the determination of twelve amino acids frequently involved in aminoacidopathies, thus enabling the diagnosis and follow-up of affected patients. The results obtained by this method are comparable to those obtained with a classical amino acid analyzer.
Badiou
Stephanie
S
Laboratoire de Biochimie, Hôpital Lapeyronie, Montpellier, France.
Lehmann
Sylvain
S
Cristol
Jean-Paul
JP
Bellet
Helene
H
D000596:Amino Acids / Q000097:blood* / Q000737:chemistry; D000634:Aminoquinolines / Q000737:chemistry; D002219:Carbamates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008661:Metabolism, Inborn Errors / Q000175:diagnosis; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results
C078178:6-aminoquinolyl-N-hydroxysuccinimidyl carbamate; D000596:Amino Acids; D000634:Aminoquinolines; D002219:Carbamates; D005456:Fluorescent Dyes; D011933:Reagent Kits, Diagnostic