title: Paradoxical decrease of mitochondrial DNA deletions in epithelial cells of active ulcerative colitis patients.
abstract: Ulcerative colitis (UC) is a condition characterized by chronic inflammation targeted at the epithelial layer. In addition to being involved in immune phenomena, UC epithelial cells exhibit decreased oxidation of butyrate, downregulation of oxidative pathway regulatory genes, and overexpression of mitochondrial (mt) genes. We investigated whether these events, which translate an altered energy metabolism, are associated with an abnormal pattern of mtDNA deletions. Highly purified colonocytes were isolated from surgically resected control, involved and uninvolved inflammatory bowel disease mucosa. The frequency, type, and number of mtDNA deletions were assessed by PCR amplification, Southern blot analysis, and cloning and sequencing of amplified DNA fragments. The 4977 mtDNA deletion was less frequent in UC than control and Crohn's disease (CD) epithelium, regardless of patient age. Several other deletions were detected, but all were less common in UC than control and CD cells. The frequency, variety, and number of mtDNA deletions were invariably lower in colonocytes isolated from inflamed mucosa than in autologous cells from noninflamed mucosa. In conclusion, in the absence of inflammation, UC colonocytes exhibit an mtDNA deletion pattern similar to that of control cells, indicating a normal response to physiological levels of oxidative stress. In active inflammation, when oxidative stress increases, the frequency, variety, and number of mtDNA deletions decrease. Because comparable abnormalities are absent in active CD, the mtDNA deletion pattern of active UC suggests that colonocytes respond uniquely to inflammation-associated stress in this condition.
authors.affiliation: Div. of Gastroenterology, Univ. Hospitals of Cleveland, Case Western Reserve Univ. School of Medicine (BRB 425 10900 Euclid Ave., Cleveland, OH 44106-4952, USA.
authors.forename: Kouhei
authors.initials: K
authors.lastname: Fukushima
authors.forename: Claudio
authors.initials: C
authors.lastname: Fiocchi
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D003093:Colitis, Ulcerative / Q000235:genetics* / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000378:metabolism; D005260:Female; D017353:Gene Deletion; D005820:Genetic Testing; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged
chemical_list: D004272:DNA, Mitochondrial