[Expression of leucocyte cell-surface antigens on colon cancer cell line HR8348].
To define the immune phenotype of colon cancer cells.



Using a panel of 40 anti-human monoclonal antibodies (MoAbs), the cells of colon cancer HR8348 were analyzed with three-color flow cytometry after direct immunofluorescent staining.



HR8348 cell line did not express CD2, CD3, CD4, CD5, CD7, CD8, TCR, CD10, CD11b, CD14, CD16, CD19, CD22, CD25, CD28, SmIg, CD33, CD35, CD36, CD41a, CD45, CD45RA, CD45RO, CD56, CD61, CD64, CD66b, CD69, CD71, CD117, CD122 and P-glycoprotein but expressed CD13, CD15, CD20, CD38, CD95 and HLA-DR.



The results demonstrate that colon cancer cell line HR8348 shares some antigenic determinants with leucocyte lineage.
Yang
Shi-long
SL
The Affiliated Children's Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China. yangshilonghz@zju.edu.cn
Tang
Yon-min
YM
Shen
Hong-qiang
HQ
Qian
Bo-qin
BQ
Song
Hua
H
Shi
Shu-wen
SW
Ning
Bo-tao
BT
Chen
Ying-hu
YH
Xu
Wei-qun
WQ
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis; D015703:Antigens, CD / Q000032:analysis*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000737:chemistry*; D006801:Humans
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D015703:Antigens, CD