Alteration of serum/glucocorticoid regulated kinase-1 (sgk-1) gene expression in rat hippocampus after transient global ischemia.
Expression of the serum/glucocorticoid regulated kinase-1 (sgk-1) gene has been reported to be induced by various stress stimuli such as hyper- or hypo-osmotic stress, UV irradiation, and heat shock stress; however, its association with global ischemia in the brain has not been studied. Using high-density oligonucleotide array analysis, we found that the sgk-1 gene was one of the genes showing alteration of expression in the rat hippocampus during 1-4 h of reperfusion after 10 min of transient global cerebral ischemia. Using TaqMan RT-PCR analysis, we confirmed an increased level of sgk-1 gene expression with statistical significance in the rat hippocampus at 2 h of reperfusion after 10 min of transient global cerebral ischemia. Using in situ hybridization (ISH) analysis, the increased level of sgk-1 gene expression was found to localize in pyramidal cells of CA2 and CA3 regions of the hippocampus after 2 h of reperfusion. These results provide an insight into the alterations of sgk-1 gene expression in the rat hippocampus after transient global cerebral ischemia.
Nishida
Yayoi
Y
Division of Genetics and Genomic Medicine, Nihon University School of Medicine, 30-1 Oyaguchi-Kamimachi, Itabashi-ku, Tokyo 173-8610, Japan.
Nagata
Toshihito
T
Takahashi
Yasuo
Y
Sugahara-Kobayashi
Megumi
M
Murata
Akiko
A
Asai
Satoshi
S
D000818:Animals; D004195:Disease Models, Animal; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D005938:Glucocorticoids / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006624:Hippocampus / Q000201:enzymology* / Q000503:physiopathology; D017874:Immediate-Early Proteins; D002546:Ischemic Attack, Transient / Q000201:enzymology* / Q000235:genetics / Q000503:physiopathology; D008297:Male; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D017966:Pyramidal Cells / Q000201:enzymology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time / Q000235:genetics; D015427:Reperfusion Injury / Q000201:enzymology* / Q000235:genetics / Q000503:physiopathology; D015854:Up-Regulation / Q000235:genetics; D000099184:Serum-Glucocorticoid Regulated Kinases
D005938:Glucocorticoids; D005947:Glucose; D017874:Immediate-Early Proteins; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D000099184:Serum-Glucocorticoid Regulated Kinases