title: Solvent-free enzymatic synthesis of 1,3-diconjugated linoleoyl glycerol optimized by response surface methodology.
abstract: An operation mode with N(2) bubbling under vacuum was employed for the solvent-free synthesis of 1,3-diconjugated linoleoyl glycerol (1,3-dCLG) from conjugated linoleic acid (CLA) catalyzed by Novozym 435. The response surface methodology (RSM) was adopted for the optimization of the reaction conditions with five major factors (incubation time, temperature, enzyme load, substrate mole ratio, and system vacuum) and three responses (CLA conversion, 1,3-dCLG yield, and acyl migration). Two sets of optimal conditions were recommended. Validation of the RSM model was verified by the good agreement between the experimental and the predicted values of 1,3-dCLG yield. Under the optimal conditions, the yield of 1,3-dCLG up to 93% was obtained. The reaction was scaled up to a production level of 100 g of 1,3-dCLG at a yield of 90.7%, indicating a promising feature of the technology in industrial applications.
authors.affiliation: Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Guo
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000465:Algorithms; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D004075:Diglycerides / Q000138:chemical synthesis*; D004789:Enzyme Activation; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D044243:Linoleic Acids, Conjugated / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D014618:Vacuum
chemical_list: C494175:1,3-dilinoleoylglycerol; D004075:Diglycerides; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D044243:Linoleic Acids, Conjugated; D012997:Solvents; C093667:Novozyme 435; D008049:Lipase