BAC-derived diagnostic markers for sex determination in asparagus.
A HindIII BAC (bacterial artificial chromosome) library of asparagus ( Asparagus officinalis L.) was established from a single male plant homozygous for the male flowering gene ( MM). The library represents approximately 5.5 haploid genome equivalents with an average insert size of 82 kb. A subset of the library (2.6 haploid genome equivalents) was arranged into DNA pools. Using nine sex-linked amplified fragment length polymorphism (AFLP) and two sequence-tagged site (STS) markers, 13 different BAC clones were identified from this part of the library. The BACs were arranged into a first-generation physical map around the sex locus. Four PCR-derived markers were developed from the BAC ends, one of which could be scored in a co-dominant way. Using a mapping population of 802 plants we mapped the BAC-derived markers to the same position close to the M gene as the corresponding AFLP and STS markers. The markers are useful for further chromosome walking studies and as diagnostic markers for selecting male plants homozygous for the M gene.
Jamsari
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A
Plant Breeding Institute, Christian-Albrechts-University of Kiel, Olshausenstrasse 40, 24098, Kiel, Germany.
Nitz
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I
Reamon-Büttner
S M
SM
Jung
C
C
D027761:Asparagus Plant / Q000235:genetics*; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D015723:Gene Library; D005819:Genetic Markers / Q000235:genetics*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D016324:Sequence Tagged Sites; D019849:Sex Determination Processes
D017931:DNA Primers; D005819:Genetic Markers