Oxidative stress responses in two populations of Laeonereis acuta (Polychaeta, Nereididae) after acute and chronic exposure to copper.
Acute (4 days) and chronic (14 days) effects of copper were evaluated on the antioxidant defenses of Laeonereis acuta (Polychaeta) collected in unpolluted (UP) and polluted (P) sites. In the acute assay (125 and 250 micro g Cu/l) superoxide dismutase (SOD) and glutathione S-transferase (GST) activities did not change, whereas catalase (CAT) increased in worms from both the sites. Lipid peroxidation was higher in copper exposed worms from the P site. In the chronic assay (62.5 micro g Cu/l) polychaetes from the P site showed enhanced activities of SOD, GST and CAT and higher contents of metallothionein-like proteins and sulfhydrils compared to worms from UP. Differences in responses between polychaetes from UP and P sites suggest that organisms from the polluted site, P, are more susceptible to oxidative stress conditions.
Geracitano
L A
LA
Departamento de Ciências Fisiológicas, Fundação Universidade Federal do Rio Grande, Rio Grande, Brazil.
Bocchetti
R
R
Monserrat
J M
JM
Regoli
F
F
Bianchini
A
A
D000704:Analysis of Variance; D000818:Animals; D001938:Brazil; D002374:Catalase; D003300:Copper / Q000633:toxicity*; D005982:Glutathione Transferase; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010101:Oxygen Consumption; D011077:Polychaeta / Q000187:drug effects* / Q000502:physiology*; D013482:Superoxide Dismutase; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D014874:Water Pollutants, Chemical; D003300:Copper; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase