Topical delivery of imiquimod to a mouse model as a novel adjuvant for human immunodeficiency virus (HIV) DNA.
We evaluated the compound imiquimod as a possible adjuvant for DNA immunization against human immunodeficiency virus (HIV). We found that gene-gun epidermal delivery of the DNA in combination with imiquimod resulted in the strongest HIV specific immune responses. The effect of imiquimod was further compared to that of recombinant granulocyte macrophage-colony stimulating factor (GM-CSF), a known DNA vaccine adjuvant. Both adjuvants were able to enhance the immune responses induced by the HIV-1 genes alone. The delivery of an adjuvant as a topical cream rather than through injections has a clear clinical benefit. We show for the first time that imiquimod can act as an adjuvant for DNA vaccination.
Zuber
Anne Kjerrström
AK
Swedish Institute for Infectious Disease Control, SE-17182 Stockholm, Sweden.
Bråve
Andreas
A
Engström
Gunnel
G
Zuber
Bartek
B
Ljungberg
Karl
K
Fredriksson
Malin
M
Benthin
Reinhold
R
Isaguliants
Maria G
MG
Sandström
Eric
E
Hinkula
Jorma
J
Wahren
Britta
B
D016915:AIDS Vaccines / Q000276:immunology*; D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000287:Administration, Topical; D000634:Aminoquinolines / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D019470:Biolistics; D016207:Cytokines / Q000096:biosynthesis / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology; D015497:HIV-1 / Q000235:genetics / Q000276:immunology; D000077271:Imiquimod; D007111:Immunity, Cellular / Q000276:immunology; D007114:Immunization; D007271:Injections, Intradermal; D007371:Interferon-gamma / Q000032:analysis / Q000096:biosynthesis; D009052:Leukemia Virus, Murine / Q000276:immunology; D051379:Mice; D013154:Spleen / Q000166:cytology / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology
D016915:AIDS Vaccines; D000276:Adjuvants, Immunologic; D000634:Aminoquinolines; D016207:Cytokines; D019444:Vaccines, DNA; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D000077271:Imiquimod