Knock-down of the 37-kDa/67-kDa laminin receptor in mouse brain by transgenic expression of specific antisense LRP RNA.
The 37-kDa/67-kDa laminin receptor (LRP/LR) plays a major role in the propagation of PrPSc, the abnormal form of the prion protein. In order to ablate the expression of LRP/LR in mouse brain we generated transgenic mice ectopically expressing antisense LRP RNA in the brain under control of the neuron-specific enolase (NSE) promoter. Hemizygous transgenic mice TgN(NSEasLRP)2 showed a significant reduction of LRP/LR protein levels in hippocampal and cerebellar brain regions. These mice might act as powerful tools to investigate the role of the laminin receptor in scrapie pathogenesis.
Leucht
Christoph
C
Laboratorium für Molekulare Biologie Genzentrum Institut für Biochemie der LMU München, Feodor-Lynen-Strasse 25, D-81377 Munich, Germany.
Vana
Karen
K
Renner-Müller
Ingrid
I
Dormont
Dominique
D
Lasmézas
Corinne Ida
CI
Wolf
Eckhard
E
Weiss
Stefan
S
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002460:Cell Line; D015870:Gene Expression; D051379:Mice / Q000235:genetics*; D008822:Mice, Transgenic; D008970:Molecular Weight; D010751:Phosphopyruvate Hydratase / Q000235:genetics; D011401:Promoter Regions, Genetic; D016372:RNA, Antisense / Q000378:metabolism*; D051381:Rats; D017474:Receptors, Laminin / Q000235:genetics / Q000378:metabolism*
D016372:RNA, Antisense; D017474:Receptors, Laminin; D010751:Phosphopyruvate Hydratase