DNA polymorphism assay distinguishes isolates of Leishmania donovani that cause kala-azar from those that cause post-kala-azar dermal Leishmaniasis in humans.
Leishmania donovani in India causes visceral infection (kala-azar) and dermal infection (post-kala-azar dermal leishmaniasis). We report here the identification of polymorphism in a well-defined genetic locus among the Leishmania parasites causing the visceral and dermal manifestations, in a comparison of 15 post-kala-azar dermal leishmaniasis and 12 kala-azar patient isolates.
Sreenivas
Gannavaram
G
Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi, India.
Raju
B V Subba
BV
Singh
Ruchi
R
Selvapandiyan
Angamuthu
A
Duncan
Robert
R
Sarkar
Dwijen
D
Nakhasi
Hira L
HL
Salotra
Poonam
P
D000818:Animals; D015342:DNA Probes; D018105:DNA, Kinetoplast / Q000032:analysis / Q000235:genetics*; D006801:Humans; D007893:Leishmania donovani / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology*; D007898:Leishmaniasis, Visceral / Q000150:complications* / Q000469:parasitology*; D011110:Polymorphism, Genetic; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D012867:Skin / Q000469:parasitology
D015342:DNA Probes; D018105:DNA, Kinetoplast; D012339:RNA, Ribosomal, 28S