[A case control study on the impact of CYP450 MSPI and GST-M1 polymorphisms on the risk of lung cancer].
OBJECTIVE
To study the correlation of polymorphisms of CYP1A1 MSPI and glutathiones S-transferase (GST-M1) independently and in combination with the risk of lung cancer.


METHODS
A case control study which included 91 cases of lung cancer and 138 controls collected from the First Affiliated Hospital of Sun Yat-sen University of Medical Sciences, Guangzhou Tumor Hospital and The Red Cross Hospital of Guangzhou or conmunity area. All subjects were investigated with a uniform questionnaire. Blood samples were collected from all cases and controls for detecting CYP1A1 MSPI and GST-M1 polymorphisms which were analyzed by PCR and RFLP.


RESULTS
It showed that there was no significant difference in frequencies of this genotypes of CYP1A1 MSPI between the two groups. The frequency of GST-M1 null (0/0) genotype was higher in the case group than in the control group, with an OR of 1.38 (95% CI 0.81 - 2.38), but there was no statistical significance. However, combination of several genotypes was strongly associated with lung cancer. There was a synergistic interaction between the m2m2 genotype of CYP1A1 MSPI and GST-M1 (0/0) genotype, with an OR of 2.47 (95% CI 1.03 - 5.90).


CONCLUSIONS
The combination of two genetic polymorphisms significantly increases the risk of lung cancer.
Wang
Bao-guo
BG
Department of Epidemiology, Guangdong Pharmacy College, Guangzhou 510224, China.
Chen
Si-dong
SD
Zhou
Wei-ping
WP
Zeng
Mian
M
Li
Zhi-biao
ZB
Cai
Xu-ling
XL
Wang
De-quan
DQ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D005260:Female; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000209:etiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012306:Risk; D012907:Smoking / Q000009:adverse effects
D019363:Cytochrome P-450 CYP1A1; D005982:Glutathione Transferase