Molecular characterization of Salmonella enterica subsp. enterica serovar Typhimurium DT1 isolates.
Salmonella Typhimurium DT1 is endemic to Finland and has caused human outbreaks since the 1960s. Domestic DT1 isolates (n=235) from 1972 to 1999 from human cases, animals and other sources, as well as foreign DT1 isolates from human cases (n=20) were analysed by molecular methods. Pulsed-field gel electrophoresis (PFGE) yielded 38 XbaI profiles. Of these, XbaI profile 10 was seen in 49% (125/255) of the isolates. Twelve IS200 profiles were obtained; the most common IS200 profile D was seen in 64% (33/52) of the isolates. Two clusters were formed by compilation of the XhaI-, BlnI- and SpeI-PFGE and IS200 profiles and possession of the serovar-specific virulence plasmid. The major cluster contained eight IS200 profiles, including IS200 profile D and XhaI profile 10, and had no virulence plasmid, and can be regarded as typical of the endemic Typhimurium DT1 infection.
Lindqvist
N
N
National Veterinary and Food Research Institute, Kuopio Department, P.O. Box 92, FIN-70701 Kuopio, Finland.
Heinikainen
S
S
Siitonen
A
A
Pelkonen
S
S
D004251:DNA Transposable Elements; D016521:Electrophoresis, Gel, Pulsed-Field; D017720:Molecular Epidemiology; D010957:Plasmids; D021521:Ribotyping; D012486:Salmonella typhimurium / Q000235:genetics*
D004251:DNA Transposable Elements