title: Caveolae as an additional route for influenza virus endocytosis in MDCK cells.
abstract: Clathrin-mediated endocytosis has been described as the primary internalization pathway for many viruses, including the influenza virus. However, caveolae, an alternative clathrin-independent endocytotic pathway, has also been described as mediating the entry of some molecules, including viruses. To address the question of pathway selection by the influenza virus, we have investigated whether the virus is internalized via clathrin-coated pits and/or caveolae in Madin Darby canine kidney (MDCK) cells. By applying pharmacological manipulations to selectively disrupt the cell internalization pathways, we found that, in MDCK cells, the influenza virus may be internalized via caveolae in addition to entry by clathrin-mediated endocytosis. However, a small contribution by another mode of entry, as recently proposed, cannot be excluded.
authors.affiliation: Department of Biochemistry, University of Coimbra, Apartado 3126, 3000 Coimbra, Portugal.
authors.forename: Isabel
authors.initials: I
authors.lastname: Nunes-Correia
authors.forename: Ana
authors.initials: A
authors.lastname: Eulálio
authors.forename: Shlomo
authors.initials: S
authors.lastname: Nir
authors.forename: Maria C
authors.initials: MC
authors.lastname: Pedroso de Lima
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000502:physiology; D021941:Caveolae / Q000378:metabolism* / Q000821:virology; D002462:Cell Membrane / Q000378:metabolism* / Q000821:virology; D002478:Cells, Cultured; D003034:Coated Pits, Cell-Membrane / Q000378:metabolism* / Q000821:virology; D018699:Coated Vesicles / Q000378:metabolism / Q000821:virology; D004285:Dogs; D004705:Endocytosis / Q000502:physiology; D004847:Epithelial Cells / Q000378:metabolism* / Q000821:virology; D005372:Filipin / Q000737:chemistry; D009975:Orthomyxoviridae / Q000378:metabolism*
chemical_list: D005372:Filipin