Isocratic reversed-phase high-performance liquid chromatographic separation of tetracyclines and flumequine controlled by a chaotropic effect.
Fast isocratic reversed-phase high-performance liquid chromatographic (RP-HPLC) separation of four tetracyclines and flumequine was obtained using a Zorbax SB-C18 column. Baseline resolution was achieved in 11 min. The peaks were narrow, well separated and without any tails although there was no chelating agents added to the mobile phase. Due to the chaotropic effect, the addition of potassium perchlorate allowed controlling the tetracyclines retention while the retention of flumequine was almost constant.
Pilorz
Karol
K
Department of Chemical Physics and Physicochemical Methods of Separation, M. Curie-Skłodowska University, M. Curie-Skłodowska Sq. 3, 20-031 Lublin, Poland. karol.pilorz@umcs.lublin.pl
Choma
Irena
I
D000900:Anti-Bacterial Agents / Q000032:analysis*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D024841:Fluoroquinolones; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D010472:Perchlorates / Q000737:chemistry; D011807:Quinolizines / Q000032:analysis*; D017670:Sodium Compounds / Q000737:chemistry; D013754:Tetracyclines / Q000032:analysis*
D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D007202:Indicators and Reagents; D010472:Perchlorates; D011807:Quinolizines; D017670:Sodium Compounds; D013754:Tetracyclines; C031068:sodium perchlorate; C012976:flumequine