Determination of 1-aminocycopropane-1-carboxylic acid (ACC) to assess the effects of ACC deaminase-containing bacteria on roots of canola seedlings.
Previously, it was proposed that plant growth-promoting bacteria that possess the enzyme, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, can reduce the amount of ethylene produced by a plant and thereby promote root elongation. To test this model, canola seeds were imbibed in the presence of the chemical ethylene inhibitor, 2-aminoethoxyvinyl glycine (AVG), various strains of plant growth-promoting bacteria, and a psychrophilic bacterium containing an ACC deaminase gene on a broad host range plasmid. The extent of root elongation and levels of ACC, the immediate precursor of ethylene, were measured in the canola seedling roots. A modification of the Waters AccQ.Tag Amino Acid Analysis Method was used to quantify ACC in the root extracts. It was found that, in the presence of the ethylene inhibitor, AVG, or any one of several ACC deaminase-containing strains of bacteria, the growth of canola seedling roots was enhanced and the ACC levels in these roots were lowered.
Penrose
D M
DM
Department of Biology, University of Waterloo, Waterloo, ON N2L 3G1, Canada.
Moffatt
B A
BA
Glick
B R
BR
D000598:Amino Acids, Cyclic / Q000032:analysis* / Q000378:metabolism; D001419:Bacteria / Q000201:enzymology*; D029688:Brassica napus; D029741:Brassica rapa / Q000737:chemistry / Q000254:growth & development / Q000382:microbiology*; D019755:Carbon-Carbon Lyases / Q000378:metabolism*; D005030:Ethylenes / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000254:growth & development* / Q000382:microbiology*; D036226:Seedlings / Q000737:chemistry / Q000254:growth & development / Q000382:microbiology
D000598:Amino Acids, Cyclic; D005030:Ethylenes; C023863:1-aminocyclopropane-1-carboxylic acid; C036216:ethylene; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases