Finb, a multiple zinc finger protein, represses transcription of the human angiotensinogen gene.
We previously identified a regulatory element at the 3'-downstream region of the human angiotensinogen (hANG) gene. Using this element as a probe by the Southwestern screening, we isolated a cDNA clone, encoding Finb, a transcriptional activator with multiple zinc finger domains. The N-terminal zinc finger domain of Finb bound to the GGATGG sequence within the regulatory element. Unexpectedly, Finb repressed transcription dependent on the regulatory element. Inspection of the 5'-flanking region in the hANG promoter identified the GGATGG-like elements, which prompted us to examine the effect of Finb on the hANG promoter activity. We also found the two Finb binding elements in the 5'-flanking region of the hANG gene by the gel shift assay, both of which were necessary for transcriptional repression of the hANG promoter. These findings suggest that Finb functions as a sequence-specific transcriptional repressor of the hANG gene.
Date
Shoichi
S
Center of Tsukuba Advanced Research Alliance, Institutes of Applied Biochemistry and Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan.
Nibu
Yutaka
Y
Yanai
Kazuyuki
K
Hirata
Jun
J
Yagami
Ken-Ichi
K
Fukamizu
Akiyoshi
A
D000808:Angiotensinogen / Q000235:genetics*; D001483:Base Sequence; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D012097:Repressor Proteins / Q000737:chemistry / Q000378:metabolism*; D020218:Response Elements / Q000235:genetics; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics*; D016335:Zinc Fingers
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C101743:RREB1 protein, human; D012097:Repressor Proteins; D014157:Transcription Factors; D000808:Angiotensinogen