title: Conversion of the Nikon C1 confocal laser-scanning head for multiphoton excitation on an upright microscope.
abstract: Most commercial laser-scanning imaging systems used for confocal fluorescence microscopy can be readily adapted for use with two-photon fluorescence excitation. We report here on the details of the conversion of the Nikon C1 (product released November 2001) with two channels of nondescanned detection of two-photon-excited fluorescence. One of the goals of the design was to utilize off-the-shelf components as much as possible to minimize the use of custom machining and electronics assembly. We also give some initial characterization of the imaging properties of the system.
authors.affiliation: Neuroscience Unit, Ottawa Health Research Institute, 725 Parkdale Avenue, Ottawa, Canada. aridsdale@ohri.ca
authors.forename: Andrew
authors.initials: A
authors.lastname: Ridsdale
authors.forename: Ileana
authors.initials: I
authors.lastname: Micu
authors.forename: Peter K
authors.initials: PK
authors.lastname: Stys
mesh_terms: D004581:Electronics; D004867:Equipment Design; D018613:Microscopy, Confocal / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D017785:Photons