NMR probing of in silico identification of anti-HPV16 E7 mAb linear peptide epitope.
A proteomics-based approach was exploited in order to individuate peptide sequences having the immunogenic potential to evoke humoral response. The epitope search utilized two parameters: the similarity level of the peptide sequence to the host's proteins, and the peptide capability to bind to the major histocompatibility complex class II molecules. By this approach, the human papillomavirus 16 E7(49-63) RAHYNIVTFCCKCDS peptide was individuated as the immunogenic epitope recognized by an anti-HPV16 E7 monoclonal antibody raised against the full-length viral oncoprotein. In this report, two-dimensional nuclear magnetic resonance spectroscopic experiments unequivocally probe the HPV16 E7 epitope individuation.
Kanduc
Darja
D
Department of Microbiology and Immunology, New York Medical College, Valhalla, NY, USA. d.kanduc@biologia.uniba.it
Fanizzi
Francesco P
FP
Lucchese
Guglielmo
G
Stevanovic
Stefan
S
Sinha
Animesh A
AA
Mittelman
Abraham
A
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D003198:Computer Simulation; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology*; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008285:Major Histocompatibility Complex / Q000276:immunology; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000276:immunology*; D027383:Papillomaviridae / Q000276:immunology*; D050725:Papillomavirus E7 Proteins
D000911:Antibodies, Monoclonal; D000939:Epitopes; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16