Coronamycins, peptide antibiotics produced by a verticillate Streptomyces sp. (MSU-2110) endophytic on Monstera sp.
Coronamycin is a complex of novel peptide antibiotics with activity against pythiaceous fungi and the human fungal pathogen Cryptococcus neoformans. It is also active against the malarial parasite, Plasmodium falciparum, with an IC(50) of 9.0 ng ml(-1). Coronamycin is produced by a verticillate Streptomyces sp. isolated as an endophyte from an epiphytic vine, Monstera sp., found in the Manu region of the upper Amazon of Peru. Bioassay-guided fractionation of the fermentation broths of this endophyte on silica gel and HPLC chromatography yielded two principal, inseparable, peptides with masses of 1217.9 and 1203.8 Da. Three other minor, but related components, are also present in the preparation. Amino acid analysis of coronamycin revealed residues of component 1, component 2, methionine, tyrosine and leucine at a ratio of 2:2:1:1:3. Other compounds with antifungal activities are also produced by this endophytic streptomycete.
Ezra
David
D
Department of Plant Sciences, Montana State University, Bozeman, MT 59717, USA.
Castillo
Uvidelio F
UF
Department of Plant Sciences, Montana State University, Bozeman, MT 59717, USA.
Strobel
Gary A
GA
Department of Plant Sciences, Montana State University, Bozeman, MT 59717, USA.
Hess
Wilford M
WM
Department of Integrated Biology, Brigham Young University, Provo, UT 84602, USA.
Porter
Heidi
H
Department of Microbiology, Brigham Young University, Provo, UT 84602, USA.
Jensen
James B
JB
Department of Microbiology, Brigham Young University, Provo, UT 84602, USA.
Condron
Margaret A M
MAM
Department of Neurology, Harvard Medical School, and Center for Neurologic Diseases, Brigham and Women's Hospital, Boston, MA 02115, USA.
Teplow
David B
DB
Department of Neurology, Harvard Medical School, and Center for Neurologic Diseases, Brigham and Women's Hospital, Boston, MA 02115, USA.
Sears
Joseph
J
Department of Chemistry, Montana State University, Bozeman, MT 59717, USA.
Maranta
Michelle
M
Novozymes Biotech Inc., 1445 Drew Ave, Davis, CA 95616, USA.
Hunter
Michelle
M
Novozymes Biotech Inc., 1445 Drew Ave, Davis, CA 95616, USA.
Weber
Barbara
B
Novozymes Biotech Inc., 1445 Drew Ave, Davis, CA 95616, USA.
Yaver
Debbie
D
Novozymes Biotech Inc., 1445 Drew Ave, Davis, CA 95616, USA.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D029064:Araceae / Q000382:microbiology* / Q000648:ultrastructure; D001419:Bacteria / Q000187:drug effects; D002460:Cell Line; D005658:Fungi / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D021261:Parasitic Sensitivity Tests; D010455:Peptides; D010963:Plasmodium falciparum / Q000187:drug effects; D017422:Sequence Analysis, DNA; D013172:Spores, Fungal / Q000648:ultrastructure; D013302:Streptomyces / Q000378:metabolism* / Q000648:ultrastructure
D000900:Anti-Bacterial Agents; D010455:Peptides