title: Lymphoepithelioma-like carcinoma of the bladder: three cases with clinicopathological and p53 protein expression study.
abstract: Lymphoepithelioma-like carcinoma of the bladder is an uncommon neoplasm, of which 49 cases have been described in the English literature, none of which has been studied for p53 protein expression. We studied three muscle-infiltrating cases of this tumor using immunohistochemical, in situ hybridization and polymerase chain reaction (PCR) methods. The three cases were positive for epithelial markers and negative for lymphoid antigens in the tumoral syncytial areas. The intensive infiltrate of small cells was negative for epithelial and positive for lymphoid markers. This population was mainly made up of cytotoxic T-lymphocytes, positive for TIA-1. p53 protein was intensely positive in more than 90% of the epithelial component nuclei, being negative in the lymphoid cells. PCR study did not show mutations on p53. Both lymphocytes and epithelium were negative for Epstein-Barr virus markers, such as the latent membrane protein and EBER (Epstein-Barr-encoded RNA). The prognosis was very good after radiotherapy and chemotherapy treatment, preserving the bladder despite the muscle infiltration. The presence of an intense cytotoxic T-lymphocyte population may be related to this good prognosis. Both aspects, p53 protein status and T-lymphoid population, had never been studied before in bladder lymphoepithelioma-like carcinoma.
authors.affiliation: Servicio de Anatomía Patológica, Hospital de León, Altos de la Nava s/n, 24008, León, Spain. pacoizq20@hotmail.com
authors.forename: Francisco Miguel
authors.initials: FM
authors.lastname: Izquierdo-García
authors.forename: Fructuoso
authors.initials: F
authors.lastname: García-Díez
authors.forename: Isabel
authors.initials: I
authors.lastname: Fernández
authors.forename: Alberto
authors.initials: A
authors.lastname: Pérez-Rosado
authors.forename: Anabel
authors.initials: A
authors.lastname: Sáez
authors.forename: Dimas
authors.initials: D
authors.lastname: Suárez-Vilela
authors.forename: Rafael
authors.initials: R
authors.lastname: Guerreiro-González
authors.forename: Manuel
authors.initials: M
authors.lastname: Benéitez-Alvarez
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology* / Q000628:therapy; D004273:DNA, Neoplasm / Q000032:analysis; D018572:Disease-Free Survival; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D016158:Genes, p53 / Q000502:physiology; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D016133:Polymerase Chain Reaction; D018714:Radiotherapy, Adjuvant; D013602:T-Lymphocytes, Cytotoxic / Q000378:metabolism / Q000473:pathology; D016896:Treatment Outcome; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D001743:Urinary Bladder / Q000601:surgery; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology* / Q000628:therapy
chemical_list: D004273:DNA, Neoplasm; D016159:Tumor Suppressor Protein p53