title: Analysis of microbial diversity in oligotrophic microbial fuel cells using 16S rDNA sequences.
abstract: Molecular ecological techniques were applied to analyze the bacterial diversity of two oligotrophic microbial fuel cells (MFCs) enriched using river water or artificial wastewater (AWW) as fuel. Denaturing gradient gel electrophoresis (DGGE) of the PCR amplified 16S rDNA showed that different microbial communities were present in the two MFCs and these were different from the river sediment used to initiate the enrichment. Nearly complete 16S rDNA was amplified and sequenced. Over 80% of the clones were Proteobacteria. Betaproteobacteria were the dominant clones (46.2%) in MFCs fed with river water, and about 64.4% of the clones in MFCs fed with AWW were Alphaproteobacteria. Actinobacteria were found only in the MFC fed with AWW, and Deltaproteobacteria, Acidobacteria, Chloroflexi and Verrucomicrobia in the MFC fed with river water. Many clones were related to uncultured bacteria, some with homology less than 95%, indicating that many novel bacteria were enriched in the oligotrophic MFCs.
authors.affiliation: Water Environment and Remediation Research Center, Korea Institute of Science and Technology, 39-1, Hawolgok dong, Sungbuk gu, Seoul 136-791, Republic of Korea.
authors.forename: Nguyet Thu
authors.initials: NT
authors.lastname: Phung
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Lee
authors.forename: Kui Hyun
authors.initials: KH
authors.lastname: Kang
authors.forename: In Seop
authors.initials: IS
authors.lastname: Chang
authors.forename: Geoffrey Michael
authors.initials: GM
authors.lastname: Gadd
authors.forename: Byung Hong
authors.initials: BH
authors.lastname: Kim
mesh_terms: D039903:Actinobacteria / Q000302:isolation & purification; D020561:Alphaproteobacteria / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D020562:Betaproteobacteria / Q000302:isolation & purification; D044822:Biodiversity; D001674:Bioelectric Energy Sources; D041862:Chloroflexi / Q000302:isolation & purification; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D020564:Deltaproteobacteria / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004734:Energy Metabolism; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014867:Water
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D014867:Water