title: Emissions from indoor dust inhibit proliferation of A549 cells and TNFalpha release from stimulated PBMCs.
abstract: Dust accumulating on hot indoor surfaces, e.g., heaters and light fixtures, are likely to emit chemicals when heated. Using in vitro techniques we have investigated biological effects of extracts from such emissions from three indoor and two outdoor dust samples heated at 50-250 degrees C. The cell cultures were a lung epithelial cell line (A549) and primary immune cells [peripheral blood mononuclear cell (PBMCs)]. We found that a 24-h incubation with extracts generated at 200 degrees C or higher inhibit both proliferation and mitochondrial activity of the epithelial cells. At non-cytotoxic concentrations, the extracts generated at 100 degrees C or higher inhibit the release of the pro-inflammatory cytokine tumor necrosis factor-alpha (TNFalpha) from lipopolysaccharide-stimulated PBMCs. The results imply that temperatures relevant for surfaces of equipment in the indoor environment cause emissions from dust that may have an impact on indoor air quality and affect the respiratory health of building occupants.
authors.affiliation: Department of Neuroscience-MTFS, Faculty of Medicine, Norwegian University of Science and Technology, Medisinsk Teknisk Forskningssenter, N-7489 Trondheim, Norway.
authors.forename: Mette
authors.initials: M
authors.lastname: Mathiesen
authors.forename: Ellen K
authors.initials: EK
authors.lastname: Pedersen
authors.forename: Olav
authors.initials: O
authors.lastname: Bjørseth
authors.forename: Tore
authors.initials: T
authors.lastname: Syversen
mesh_terms: D016902:Air Pollution, Indoor / Q000009:adverse effects*; D002455:Cell Division; D002460:Cell Line; D016207:Cytokines / Q000032:analysis; D004391:Dust; D004847:Epithelial Cells; D006358:Hot Temperature; D006801:Humans; D007249:Inflammation; D008168:Lung / Q000166:cytology / Q000276:immunology / Q000473:pathology*; D008928:Mitochondria / Q000502:physiology; D012140:Respiratory Tract Diseases / Q000503:physiopathology
chemical_list: D016207:Cytokines; D004391:Dust