Regulation of calcium signalling by 1-O-alkylglycerols in human Jurkat T lymphocytes.
We studied the role of natural occurring 1-O-alkylglycerols on the calcium signalling in Jurkat T-cells. Alkylglycerols evoked an increase in free intracellular calcium concentration [Ca2+]i, in a dose-dependent manner. When the experiments were performed in calcium-free buffer, the alkylglycerol response on the rise of [Ca2+]i was wholly abolished compared with the one in calcium-containing buffer, suggesting that these etherlipids induce a calcium influx by the opening of Ca2+ channels. We further employed inhibitors of voltage-gated calcium channels. We observed that omega-conotoxin, a blocker of N-type voltage-activated Ca2+ channels, but not verapamil, a blocker of L-type voltage-activated Ca2+ channels, curtailed significantly the calcium rise evoked by the lipid agents. Alkylglycerols also induced plasma membrane depolarisation, known to be involved in the opening of the voltage-gated calcium channels. Our study shows that alkylglycerols increase [Ca2+]i influx in human Jurkat T-cells possibly by modulating the permeability of calcium channels.
Pédrono
Frédérique
F
Laboratoire de Pharmacologie Moléculaire, Faculté des Sciences Pharmaceutiques et Biologiques, 2 avenue du Pr Léon Bernard, 35043 Rennes cedex, France.
Khan
Naïm A
NA
Legrand
Alain B
AB
D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020013:Calcium Signaling / Q000187:drug effects*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D005990:Glycerol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006801:Humans; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology; D019169:Jurkat Cells / Q000187:drug effects* / Q000378:metabolism; D008099:Liver / Q000737:chemistry; D008564:Membrane Potentials / Q000187:drug effects; D012754:Sharks; D014020:Tissue Extracts / Q000494:pharmacology*; D020960:omega-Conotoxins / Q000494:pharmacology
D002121:Calcium Channel Blockers; D014020:Tissue Extracts; D020960:omega-Conotoxins; D005990:Glycerol