title: Neutrophil and platelet dynamics at organ level after cardiopulmonary bypass: an in vivo study in neonatal pigs.
abstract: The aim was to investigate if organ dysfunction is a consequence of cell accumulation in the tissue and whether this accumulation is caused by the cardiopulmonary bypass (CPB) procedure. Twenty-six piglets were used in the sham group (sternotomy, n=12) or in the CPB group (sternotomy, CPB, n=14). Isotope-labeled autologous (99m)Tc-neutrophils (PMNs) and (111)In-platelets were infused and dynamically followed at organ level with a gamma camera before, during, and 4 h after termination of CPB. The CPB group showed a 49% increase in (99m) Tc-PMNs in the kidneys in the postoperative period compared to a decrease of 2% in the sham group. A less marked decrease was observed in the lungs and peripheral blood between the two groups. The increased radioactivity at organ level post-CPB could be due to changes in flow, extraction in the organ or accumulation of cells, especially in the kidneys and lungs, and might contribute to temporary organ dysfunction postoperatively.
authors.affiliation: Department of Anaesthesiology, Aarhus University Hospital, Denmark. brixchr@dadlnet.dk
authors.forename: V
authors.initials: V
authors.lastname: Brix-Christensen
authors.forename: M
authors.initials: M
authors.lastname: Rheling
authors.forename: C
authors.initials: C
authors.lastname: Flø
authors.forename: H
authors.initials: H
authors.lastname: Ravn
authors.forename: V
authors.initials: V
authors.lastname: Hjortdal
authors.forename: J
authors.initials: J
authors.lastname: Marqversen
authors.forename: N
authors.initials: N
authors.lastname: Andersen
authors.forename: E
authors.initials: E
authors.lastname: Tønnesen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001792:Blood Platelets / Q000000981:diagnostic imaging / Q000502:physiology*; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D004195:Disease Models, Animal; D015902:Gamma Cameras; D007204:Indium / Q000097:blood; D007668:Kidney / Q000098:blood supply; D008099:Liver / Q000098:blood supply; D008168:Lung / Q000098:blood supply; D009504:Neutrophils / Q000000981:diagnostic imaging / Q000502:physiology*; D009566:Nitrates / Q000097:blood; D011877:Radionuclide Imaging; D013552:Swine; D013667:Technetium / Q000097:blood
chemical_list: D009566:Nitrates; D007204:Indium; D013667:Technetium; C089767:indium nitrate