title: Immobilized 2-(4-hydrazinocarbonylphenyl)-4,5-diphenylimidazole as solid phase luminophore in peroxyoxalate chemiluminescence.
abstract: A new luminophore for application in peroxyoxalate chemiluminescence is presented. An analogue of the well-known chemiluminescence compound lophine, i.e. 2-(4-hydrazinocarbonylphenyl)-4,5-diphenylimidazole (HCPI), has been covalently immobilized to controlled pore glass and a porous methacrylate resin. By using this reagent in a solid phase detection reactor, sensitive determinations of hydrogen peroxide have been demonstrated. In homogeneous solution HCPI emits poorly as a result of 1,1'-oxalyldiimidazole excitation, but when immobilized its efficiency is almost comparable to highly efficient luminophores such as 3-aminofluoranthene. Linearity extends in the single stream flow system over several orders of magnitude with both materials. The limit of detection was 1 nmol/l (10 fmole injected), when using the porous methacrylate support.
authors.affiliation: Department of Analytical Chemistry, University of Umeå, S-901 87, Umeå, Sweden.
authors.forename: E
authors.initials: E
authors.lastname: Pontén
authors.forename: P
authors.initials: P
authors.lastname: Appelblad
authors.forename: M
authors.initials: M
authors.lastname: Stigbrand
authors.forename: K
authors.initials: K
authors.lastname: Irgum
authors.forename: K
authors.initials: K
authors.lastname: Nakashima