title: Calcium signaling in isolated skeletal muscle fibers investigated under "Silicone Voltage-Clamp" conditions.
abstract: In skeletal muscle, release of calcium from the sarcoplasmic reticulum (SR) represents the major source of cytoplasmic Ca2+ elevation. SR calcium release is under the strict command of the membrane potential, which drives the interaction between the voltage sensors in the t-tubule membrane and the calcium-release channels. Either detection or control of the membrane voltage is thus essential when studying intracellular calcium signaling in an intact muscle fiber preparation. The silicone-clamp technique used in combination with intracellular calcium measurements represents an efficient tool for such studies. This article reviews some properties of the plasma membrane and intracellular signals measured with this methodology in mouse skeletal muscle fibers. Focus is given to the potency of this approach to investigate both fundamental aspects of excitation-contraction coupling and potential alterations of intracellular calcium handling in some muscle diseases.
authors.affiliation: Laboratoire de Physiologie des Eléments Excitables, Université Claude Bernard Lyon 1, UMR CNRS 5123, F69622 Villeurbanne, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Collet
authors.forename: Sandrine
authors.initials: S
authors.lastname: Pouvreau
authors.forename: Laszlo
authors.initials: L
authors.lastname: Csernoch
authors.forename: Bruno
authors.initials: B
authors.lastname: Allard
authors.forename: Vincent
authors.initials: V
authors.lastname: Jacquemond
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology*; D002478:Cells, Cultured; D006801:Humans; D008564:Membrane Potentials / Q000502:physiology*; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000502:physiology*; D018408:Patch-Clamp Techniques / Q000379:methods*; D012828:Silicones
chemical_list: D012828:Silicones; D002118:Calcium