[Immunohistochemical study of fibronectin in multiple organ dysfunction syndrome in elderly rats].
OBJECTIVE
To study the characteristic of endothelial injuries and the mechanisms of coagulation disorder in multiple organ dysfunction syndrome in elderly rats by observing the distribution and the intensity of fibronectin (FN) expression in important viscerae including the heart, brain, lung, kidney, liver.


METHODS
Fifty Wistar rats (one half of them were 3 months old, another half 20 months old) were anaesthetized and cecal ligation puncture (CLP) was done. They were defined as MODS group and MODSE group. Forty Wistar rats (one half of them 3 months old, another half 20 months old) were anaesthetized and laparotomy was done as sham operation group (SOG) and sham operation group in elderly (SOGE). The heart, brain, lung, liver and kidney were harvested and were examined histologically at 6, 12, 24, 48 or 72 hours after operation. They were also stained immunohistochemically by using anti-FN as the first antibody.


RESULTS
The expression of FN on the surfaces of vascular endothelial cells in hearts, brains, lungs, livers, kidneys was weakly positive or positive in MODSE, MODS or SOGE group, but it was positive or strongly positive in SOG group. The differences were significant (P<0.05).


CONCLUSIONS
The injury of endothelial cells and the disorders of coagulation system are observed in MODSE, MODS, SOGE groups, especially in MODSE.
Li
Yan-rong
YR
Department of Forensic Pathology, Sun Yat-sen University, Guangzhou 510080, Guangdong, China.
Jing
Hua-lan
HL
Cheng
Jian-ding
JD
Luo
Si-min
SM
Jiang
Fu-xue
FX
Zhang
Yi-jun
YJ
D000367:Age Factors; D000818:Animals; D001923:Brain Chemistry; D004195:Disease Models, Animal; D005260:Female; D005353:Fibronectins / Q000032:analysis*; D007150:Immunohistochemistry; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D008297:Male; D009102:Multiple Organ Failure / Q000378:metabolism* / Q000473:pathology; D009206:Myocardium / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
D005353:Fibronectins