title: A PCR-based assay to detect hAT-like transposon sequences in plants.
abstract: Despite their potential as endogenous tools for forward and reverse genetics, members of the hobo, Ac, Tam3 (or hAT) superfamily of transposable elements have been characterized in but a limited number of plant species. To expedite their isolation, we developed a PCR-based assay for the detection of hAT-like transposon sequences in plants which was applied to isolate and initially characterize such sequences from Petunia hybrida, Phaseolus vulgaris, Bambusa vulgaris, Brassica napus and Rhododendron simsii.
authors.affiliation: Department of Plant Systems Biology, Faculty of Sciences, Ghent University, K. Ledeganckstraat 35, 9000 Ghent, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: De Keukeleire
authors.forename: S
authors.initials: S
authors.lastname: De Schepper
authors.forename: J
authors.initials: J
authors.lastname: Gielis
authors.forename: T
authors.initials: T
authors.lastname: Gerats
mesh_terms: D004251:DNA Transposable Elements / Q000235:genetics*; D063245:Plant Development; D016133:Polymerase Chain Reaction
chemical_list: D004251:DNA Transposable Elements