A novel symbiotic nitrogen-fixing member of the Ochrobactrum clade isolated from root nodules of Acacia mangium.
Ten strains of root nodule bacteria were isolated from the nodules of Acacia mangium grown in the Philippines and Thailand. Partial sequences (approx. 300 bp) of the 16S rRNA gene of each isolate were analyzed. The nucleotide sequences of strain DASA 35030 indicated high homology (>99%) with members of the genus Ochrobactrum in Brucellaceae, although the sequences of other isolates were homologous to those of two distinct genera Bradyrhizobium and Rhizobium. The strain DASA 35030 was strongly suggested to be a strain of Ochrobactrum by full length sequences of the 16S rRNA gene, fatty acids composition, G+C contents of the DNA, and other physiological characteristics. Strain DASA 35030 induced root nodules on A. mangium, A. albida and Paraserianthes falcataria. The nodules formed by strain DASA 35030 fixed nitrogen and the morphology of the nodules is the same as those of nodules formed by the other isolates. This is the first report that the strain of Ochrobactrum possesses complete symbiotic ability with Acacia.
Ngom
Amy
A
Laboratory of Plant and Microbe Interaction, Department of Chemistry and BioScience, Faculty of Science, Kagoshima University, Kagoshima 890-0065, Japan.
Nakagawa
Yasuyoshi
Y
Sawada
Hiroyuki
H
Tsukahara
Junzo
J
Wakabayashi
Shiro
S
Uchiumi
Toshiki
T
Nuntagij
Achara
A
Kotepong
Somsak
S
Suzuki
Akihiro
A
Higashi
Shiro
S
Abe
Mikiko
M
D000045:Acacia / Q000382:microbiology* / Q000648:ultrastructure; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D009586:Nitrogen Fixation / Q000235:genetics; D042041:Ochrobactrum / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D010679:Philippines; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology / Q000648:ultrastructure; D011809:Quinones / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D013785:Thailand
D004269:DNA, Bacterial; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S