Development of a multiplex microarray microsystem.
A hybrid multiplex microarray microsystem has been developed that consists of 32 individually addressable array reaction chambers, supporting the use of multichannel pipettes for addition of up to 8 samples simultaneously. Discrimination between Campylobacter jejuni and Campylobacter coli bacteria was observed in DNA samples containing Campylobacter spp., with the same specificity and sensitivity as when compared to a full-size microarray. The spinloaded multiplex microarray microsystem described provides a novel and convenient test format for simultaneous low-density microarray analysis and is universally adaptable to other DNA, protein or small molecule microarray based applications.
Keramas
Georgios
G
MIC - Department of Micro and Nanotechnology, Technical University of Denmark, DTU - Building 345 east, DK-2800 Kongens Lyngby, Denmark.
Perozziello
Gerardo
G
Geschke
Oliver
O
Christensen
Claus B V
CB
D001672:Biocompatible Materials; D017000:Campylobacter coli / Q000235:genetics; D016123:Campylobacter jejuni / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005453:Fluorescence; D005898:Glass / Q000737:chemistry; D008832:Microchemistry / Q000295:instrumentation* / Q000379:methods; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods; D011108:Polymers / Q000737:chemistry; D012680:Sensitivity and Specificity
D001672:Biocompatible Materials; D004269:DNA, Bacterial; D011108:Polymers