Chung-Yeul-Gue-Soup-Sa-Gan-Tang, traditional Korean medicine, enhances CD4(+) T cell activities and modulates Th1/Th2 lineage development.
Chung-Yeul-Gue-Soup-Sa-Gan-Tang (CYT), a traditional Korea herbal medicine, has been widely used in Korea for the treatment of various immunological disorders, including allergic asthma. In this study, CYT was examined in vitro and tested for possible immunological effects. The results demonstrated that CYT had no mitogenic effects on unstimulated CD4(+) T cells, but rather increased CD4(+) T cell proliferation upon activation with anti-CD3/CD28 antibody. Under the Th0 condition, CYT also enhanced expression of interleukin (IL)-2 in purified murine CD4(+) T cells assayed by real-time PCR, suggesting that CYT moderately increases the activity of helper T cells upon T cell receptor ligation under the neutral condition. However, the Th1 cells were overpopulated following CYT treatment under the Th1 condition, while Th2 cells were under-populated in the Th2 driven condition. In addition, under Th1/Th2-skewed conditions, the levels of IL-4 were considerably decreased, while the expression of T-bet and interferon-gamma were increased with CYT treatment. Thus, CYT enhances Th1 lineage development from naive CD4(+) T cells both by increasing Th1 specific cytokine secretion and repressing Th2 specific cytokine production. These results suggest that CYT is a desirable agent for the correction of Th2 dominant pathological disorders.
Ko
Eunjung
E
Department of Physiology, College of Oriental Medicine, Kyunghee University, Seoul, Korea.
Park
Jae-Woo
JW
Rho
Samwoong
S
Cho
Chongwoon
C
Park
Seongkyu
S
Ko
Seonggyu
S
Lee
Yongwon
Y
Hong
Moo-Chang
MC
Shin
Min-Kyu
MK
Ryu
Ki-Won
KW
Bae
Hyunsu
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D019070:Cell Lineage; D002470:Cell Survival; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D007723:Korea; D008213:Lymphocyte Activation / Q000276:immunology; D008518:Medicine, East Asian Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology; D018417:Th1 Cells / Q000187:drug effects* / Q000378:metabolism; D018418:Th2 Cells / Q000187:drug effects* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D004365:Drugs, Chinese Herbal; D007376:Interleukin-2; D015847:Interleukin-4; D007371:Interferon-gamma