Dibenzocyclooctadiene lignans from Schisandra chinensis protect primary cultures of rat cortical cells from glutamate-induced toxicity.
A methanolic extract of dried Schisandra fruit (Schisandra chinensis Baill.; Schisandraceae) significantly attenuated the neurotoxicity induced by L-glutamate in primary cultures of rat cortical cells. Five dibenzocyclooctadiene lignans (deoxyschisandrin, gomisin N, gomisin A, schisandrin, and wuweizisu C) were isolated from the methanolic extract; their protective effects against glutamate-induced neurotoxicity were then evaluated. Among the five lignans, deoxyschisandrin, gomisin N, and wuweizisu C significantly attenuated glutamate-induced neurotoxicity as measured by 1). an inhibition in the increase of intracellular [Ca(2+)]; 2). an improvement in the glutathione defense system, the level of glutathione, and the activity of glutathione peroxidase; and 3). an inhibition in the formation of cellular peroxide. These results suggest that dibenzocyclooctadiene lignans from Schisandra chinensis may possess therapeutic potential against oxidative neuronal damage induced by excitotoxin.
Kim
So Ra
SR
College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul, Korea.
Lee
Mi Kyeong
MK
Koo
Kyung Ah
KA
Kim
Seung Hyun
SH
Sung
Sang Hyun
SH
Lee
Na Gyong
NG
Markelonis
George J
GJ
Oh
Tae H
TH
Yang
Jae Ho
JH
Kim
Young Choong
YC
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D034242:Cyclooctanes / Q000494:pharmacology*; D004149:Dioxoles / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D018698:Glutamic Acid / Q000633:toxicity*; D007424:Intracellular Fluid / Q000378:metabolism; D017705:Lignans / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009498:Neurotoxins / Q000633:toxicity; D010545:Peroxides / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D011083:Polycyclic Compounds / Q000494:pharmacology; D051381:Rats; D032141:Schisandra / Q000737:chemistry*
D034242:Cyclooctanes; D004149:Dioxoles; D017705:Lignans; D018696:Neuroprotective Agents; D009498:Neurotoxins; D010545:Peroxides; D010936:Plant Extracts; D011083:Polycyclic Compounds; C015499:schizandrin B; D018698:Glutamic Acid; C033585:schizandrol B; C034734:schizandrin A; C031409:schizandrin C; C011105:schizandrin; D002118:Calcium