Attenuation of diurnal rhythms in plasma levels of melatonin and cortisol, and hypothalamic contents of vasotocin and isotocin mRNAs in pre-spawning chum salmon.
In the present study, diurnal changes in plasma levels of melatonin and cortisol, and hypothalamic contents of neurohypophysial hormone mRNAs were examined in pre-spawning chum salmon, Oncorhynchus keta. From late November to early December, homing fish were captured at two sites along their migratory pathway on the Sanriku coast, Japan. Fish captured in the seawater (SW) environment were transferred to SW aquaria, and fish captured in the freshwater (FW) environment were to FW aquaria. They were maintained under natural photoperiod of approximately 10L:14D and sacrificed at 4-h interval through 24-h period. Plasma levels of melatonin were determined by radioimmunoassay, while cortisol levels were determined by enzyme immunoassay. Hypothalamic contents of vasotocin and isotocin mRNAs were determined by quantitative dot-blot hybridization assay. The melatonin levels showed weak nocturnal elevations in the SW and FW males, and FW females. The levels were maximal at 22:00 and minimal at 10:00 or 14:00, however the amplitudes were smaller than those reported in the previous studies using immature salmonids. The levels of vasotocin and isotocin mRNAs were higher in the males at all time points. The mRNA levels, however, did not show any diurnal variations in either of group. The same applied to plasma cortisol levels. These results indicate that the diurnal endocrine rhythms were attenuated in pre-spawning chum salmon, in contrast to the prominent diurnal rhythms in immature salmonids.
Saito
Daisuke
D
Division of Biological Sciences, Hokkaido University Graduate School of Science, Sapporo, Hokkaido 060-0810, Japan. djsaito@sci.hokudai.ac.jp
Shi
Qiong
Q
Ando
Hironori
H
Urano
Akihisa
A
D000818:Animals; D001835:Body Weight / Q000502:physiology; D002940:Circadian Rhythm / Q000502:physiology*; D005260:Female; D006854:Hydrocortisone / Q000097:blood*; D006908:Hydroxyprogesterones / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism*; D017403:In Situ Hybridization; D008297:Male; D008550:Melatonin / Q000097:blood*; D017685:Oncorhynchus keta / Q000502:physiology*; D010121:Oxytocin / Q000031:analogs & derivatives* / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D011863:Radioimmunoassay; D014668:Vasotocin / Q000378:metabolism*
D006908:Hydroxyprogesterones; D012333:RNA, Messenger; C007433:17,20-dihydroxy-4-pregnen-3-one; D010121:Oxytocin; C008215:isotocin; D008550:Melatonin; D014668:Vasotocin; D006854:Hydrocortisone