[Protective immunity induced by recombinant signaling protein 14-3-3 vaccine of Schistosoma japonicum].
OBJECTIVE
To evaluate the immunoprotective effect of Schistosoma japonicum (Chinese strain) recombinant signaling protein 14-3-3 (rSj14-3-3), and to observe the synergism of rSj14-3-3 and rSjGST proteins as candidate vaccine and the effect of gamma delta-T cells activated by Mtb against Schistosoma japonicum.


METHODS
BALB/c mice immunized with rSj14-3-3 and rSjGST purified through SDS-PAGE, electroelution and dialysis were challenged by cercaria infection. Six weeks after challenging infection, the mice were killed and the worm and egg reduction rates were calculated.


RESULTS
Worm reduction rate was found to be 32.20% in rSj14-3-3 + Freund adjuvant group, 31.10% in rSj14-3-3 + rSjGST + Freund adjuvant group, 27.96% in rSj14-3-3 + Mtb group, 26.00% in rSj14-3-3 + rSjGST + Mtb group, and 27.10% in rSjGST + Mtb group, respectively, number of eggs in liver tissue was reduced by 50.40%, 53.30%, 51.10%, 58.60% and 51.30%, respectively.


CONCLUSIONS
rSj14-3-3 could induce partial immunity against Schistosoma japonicum in BALB/c mice, and might serve as a candidate vaccine; gamma delta-T cell activated by Mtb played a role in anti-Schistosoma japonicum similar to the immune reactions induced by Freund adjuvant, but no synergistic effect combined with rSjGST was observed.
Liu
Qing-zhong
QZ
Department of Pathobiology, Anhui Medical University, Hefei 230032.
Shen
Ji-long
JL
D048948:14-3-3 Proteins; D000818:Animals; D005260:Female; D005982:Glutathione Transferase / Q000276:immunology; D015801:Helminth Proteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology / Q000517:prevention & control*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D014446:Tyrosine 3-Monooxygenase / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*
D048948:14-3-3 Proteins; D015801:Helminth Proteins; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D014446:Tyrosine 3-Monooxygenase; D005982:Glutathione Transferase