Crystallization and preliminary X-ray crystallographic analysis of Plasmodium falciparum S-adenosyl-L-homocysteine hydrolase.
S-adenosyl-l-homocysteine hydrolase from a malaria parasite Plasmodium falciparum (PfSAHH) has been crystallized by the vapor diffusion method. The crystals belong to an orthorhombic space group P212121 with the cell dimensions of a = 76.66 A, b = 86.31 A, and c = 335.6 A. There are four subunits (one tetramer) per asymmetric unit. X-ray diffraction data have been collected up to 2.8 A resolution. Self-rotation function studies suggest that the tetrameric PfSAHH molecule has the 222 point group symmetry.
Tanaka
Nobutada
N
School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan.
Kusakabe
Yoshio
Y
Shiraiwa
Katsura
K
Sakamoto
Yasumitsu
Y
Nakanishi
Masayuki
M
Kitade
Yukio
Y
Nakamura
Kazuo T
KT
D043383:Adenosylhomocysteinase / Q000737:chemistry*; D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D010963:Plasmodium falciparum / Q000201:enzymology*; D015800:Protozoan Proteins / Q000737:chemistry*
D015800:Protozoan Proteins; D043383:Adenosylhomocysteinase