A novel, ultra-large xylanolytic complex (xylanosome) secreted by Streptomyces olivaceoviridis.
The production of a novel, very large xylanolytic complex (xylanosome) by Streptomyces olivaceoviridis E-86 is reported. The molecular weight was approx. 1200 kDa as determined by native gradient gel electrophoresis. Corncob xylan was the best inducer of xylanosome formation. The xylanosome was produced when the organism was grown for 5 d between pH 4.5-6 and at 27.5 to 35 degrees C.
Jiang
Zheng-Qiang
ZQ
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China.
Deng
Wei
W
Li
Li-Te
LT
Ding
Chang-He
CH
Kusakabe
Isao
I
Tan
Sze-Sze
SS
D018929:Cell Culture Techniques / Q000379:methods*; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis* / Q000737:chemistry; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000096:biosynthesis* / Q000737:chemistry; D013045:Species Specificity; D013302:Streptomyces / Q000737:chemistry / Q000145:classification / Q000201:enzymology* / Q000254:growth & development; D013696:Temperature; D014990:Xylans / Q000145:classification / Q000378:metabolism*
D009097:Multienzyme Complexes; D014990:Xylans; D043364:Endo-1,4-beta Xylanases