Human papillomavirus frequency in oral epithelial lesions.
BACKGROUND
Oral human papillomavirus (HPV) prevalence varies according to geographical occurrence, the type of lesion, and the method of diagnosis. The polymerase chain reaction method (PCR) appears to be more sensitive and can be easily applicable to epidemiologic studies.


OBJECTIVE
To determine the frequency of HPV and its genotypes in oral lesions among patients attending a reference clinic of a university hospital.


METHODS
PCR was performed to identify HPV DNA from samples of oral epithelial lesions in 80 patients. For HPV DNA amplification, MY09/MY11 consensus primers were used and specific genotypes were identified through restriction fragment of length polymorphism (RFLP) pattern.


RESULTS
HPV DNA was present in 11.3% of patients, and the identified genotypes were 6b, MM4 (W13B), and MM9 (PAP238A).


CONCLUSIONS
HPV DNA frequency in patients with oral epithelial lesions was 11.3%. The genotypes MM4 and MM9 are uncommon in oral lesions, and they are characterized as high-risk HPV types in those types of lesions.
Lazzari
Carmen Maria
CM
Federal University of Rio Grande do Sul, Porto Alegre, Brazel. carlaz44@terra.com.br
Krug
Luciano Percival
LP
Quadros
Onofre Francisco
OF
Baldi
Cristiano Bozzetti
CB
Bozzetti
Mary Clarisse
MC
D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000032:analysis; D004848:Epithelium / Q000821:virology; D006801:Humans; D008875:Middle Aged; D009059:Mouth Diseases / Q000821:virology*; D009061:Mouth Mucosa / Q000821:virology; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D030361:Papillomavirus Infections / Q000821:virology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D004279:DNA, Viral