title: Development of multiplex DNA electronic microarrays using a universal adaptor system for detection of single nucleotide polymorphisms.
abstract: The NanoChip electronic microarray is designed for the rapid detection of genetic variation in research and clinical diagnosis. We have developed a multiplex electronic microarray assay, specific for single nucleotide polymorphism (SNP) genotyping and mutation detection, using universal adaptor sequences tailed to the 5' end of PCR primers specific to each target. PCR products, amplified by primers directed to the universal adaptor sequence, are immobilized on the microarray either directly or via capture oligonucleotides complementary to the universal adaptor sequence. This simple modification results in a significant increase in fidelity with improved specificity and accuracy. In addition, the multiplexing of genetic variant detection allows increased throughput and significantly reduced cost per assay. This general schema can also be applied to other microarray and macroarray formats.
authors.affiliation: SAIC-Frederick, National Cancer Institute, Frederick, MD, USA. shirleytsang@ncifcrf.gov
authors.forename: Shirley
authors.initials: S
authors.lastname: Tsang
authors.forename: Zhonghe
authors.initials: Z
authors.lastname: Sun
authors.forename: Claudia
authors.initials: C
authors.lastname: Stewart
authors.forename: Nicole
authors.initials: N
authors.lastname: Lum
authors.forename: Casey
authors.initials: C
authors.lastname: Frankenberger
authors.forename: Marianne
authors.initials: M
authors.lastname: Subleski
authors.forename: Lynn
authors.initials: L
authors.lastname: Rasmussen
authors.forename: David J
authors.initials: DJ
authors.lastname: Munroe
mesh_terms: D000818:Animals; D015342:DNA Probes; D051379:Mice; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide
chemical_list: D015342:DNA Probes