Identification of signature genes for rapid and specific characterization of Yersinia pestis.
Polymerase chain reaction (PCR) amplification of DNA-based unique markers, the signature sequences, is ideal for rapid detection and identification of pathogens. We described the discovery of twenty-eight signature genes of Yersinia pestis by DNA microarray-based comparative genome hybridization in conjunction with PCR validation. Three pairs of Y. pestis-specific primers designed from signature genes were demonstrated to have the expected specificity to this target bacterium, without cross-reaction with the closely related Y. pseudotuberculosis or a large collection of genomic DNAs from other organisms.
Zhou
Dongsheng
D
National Center for Biomedical Analysis, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Bejing, China.
Han
Yanping
Y
Dai
Erhei
E
Pei
Decui
D
Song
Yajun
Y
Zhai
Junhui
J
Du
Zongmin
Z
Wang
Jin
J
Guo
Zhaobiao
Z
Yang
Ruifu
R
D001426:Bacterial Proteins / Q000235:genetics*; D017931:DNA Primers; D016680:Genome, Bacterial; D006801:Humans; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D015010:Yersinia pestis / Q000145:classification* / Q000235:genetics; D015011:Yersinia pseudotuberculosis / Q000145:classification / Q000235:genetics
D001426:Bacterial Proteins; D017931:DNA Primers