Evaluation of the enantiomeric selectivity in the chiral ligand-exchange chromatography of amino acids by a computational model.
The chromatographic resolution of enantiomeric amino acids is accomplished on a reversed phase column using aqueous mobile phase containing the chiral reagent N,N-dimethyl-S-phenylalanine-Cu(II). The separation is a result of the whole interaction between the diastereomeric complex surface and the mixed stationary phase realized by the dynamic coating of the RP-18 carbon chains layer. The elution order seems to be related to the different water coordination capability on copper ion in the formation of the mixed ternary complexes.
Natalini
Benedetto
B
Dipartimento di Chimica e Tecnologia del Farmaco, Università degli Studi di Perugia, Via del Liceo 1, 06123 Perugia, Italy. natalini@iris.chimfarm.unipg.it
Marinozzi
Maura
M
Sardella
Roccaldo
R
Macchiarulo
Antonio
A
Pellicciari
Roberto
R
D000596:Amino Acids / Q000032:analysis*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D008024:Ligands; D008958:Models, Molecular; D013237:Stereoisomerism
D000596:Amino Acids; D008024:Ligands