title: Enzymatic nanolithography of a self-assembled oligonucleotide monolayer on gold.
abstract: This paper describes a simple strategy to biochemically manipulate a surface at the nanoscale by enzyme dip-pen nanolithography using an endonuclease (DNase I) that is directly patterned on a self-assembled monolayer presenting a terminal oligonucleotide. Physisorbed nanopatterns of DNase I carried out nanoscale enzymology at the surface creating oligonucleotide patterns with the fidelity of the patterned enzyme because of the affinity of the enzyme for the immobilized, oligonucleotide substrate.
authors.affiliation: Department of Biomedical Engineering, Center for Biologically Inspired Materials and Material Systems, Duke University, Durham, North Carolina 27708-0281, USA.
authors.forename: Jinho
authors.initials: J
authors.lastname: Hyun
authors.forename: Jeonghan
authors.initials: J
authors.lastname: Kim
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Craig
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Chilkoti
mesh_terms: D003850:Deoxyribonuclease I / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods; D036103:Nanotechnology / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D006046:Gold; D003850:Deoxyribonuclease I