Specific histone patterns and acetylase/deacetylase activity at the breakpoint-cluster region of the human MLL gene.
The MLL gene breakpoint-cluster region (BCR) is a known hot-spot for chromosomal translocations in human leukemias. We mapped core histone modifications and histone H1 along the MLL gene in Jurkat cells and human CD34(+) progenitor blood cells by chromatin immunoprecipitation. Within the BCR, we found specific histone patterns that were different from other genomic regions and a histone H1-free fragment at the telomeric end. Core histone acetylase/deacetylase activities were also found within the BCR. In the studied cell models, chromatin components at the MLL BCR suggest an asymmetric organization that may influence early molecular events eventually leading to chromosomal translocations.
Khobta
Andriy
A
G. Moruzzi Department of Biochemistry, Alma Mater Studiorum University of Bologna, Bologna, Italy.
Carlo-Stella
Carmelo
C
Capranico
Giovanni
G
D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism*; D018952:Antigens, CD34 / Q000378:metabolism; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D019457:Chromosome Breakage / Q000235:genetics*; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins / Q000235:genetics*; D016195:G2 Phase / Q000502:physiology; D051548:Histone Acetyltransferases; D006655:Histone Deacetylases / Q000378:metabolism*; D011495:Histone-Lysine N-Methyltransferase; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D008938:Mitosis / Q000502:physiology; D051788:Myeloid-Lymphoid Leukemia Protein; D011519:Proto-Oncogenes; D013379:Substrate Specificity; D014157:Transcription Factors
D018952:Antigens, CD34; D002843:Chromatin; D004268:DNA-Binding Proteins; D006657:Histones; C497422:KMT2A protein, human; D014157:Transcription Factors; D051788:Myeloid-Lymphoid Leukemia Protein; D011495:Histone-Lysine N-Methyltransferase; D000123:Acetyltransferases; D051548:Histone Acetyltransferases; D006655:Histone Deacetylases