title: Evidence that vector transmission of a plant virus requires conformational change in virus particles.
abstract: Transmission of Cucumber necrosis virus (CNV) by zoospores of its fungal vector, Olpidium bornovanus, involves specific adsorption of virus particles onto the zoospore plasmalemma prior to infestation of cucumber roots by virus-bound zoospores. Previous work has shown that specific components of both CNV and zoospores are required for successful CNV/zoospore recognition. Here, we show that limited trypsin digestion of CNV following in vitro CNV/zoospore binding assays, results in the production of specific proteolytic digestion products under conditions where native CNV is resistant. The proteolytic digestion pattern of zoospore-bound CNV was found to be similar to that of swollen CNV particles produced in vitro, suggesting that zoospore-bound CNV is in an altered conformational state, perhaps similar to that of swollen CNV. We show that an engineered CNV mutant (Pro73Gly) in which a conserved proline residue (Pro73) in the beta-annulus of the CP arm is changed to glycine is resistant to proteolysis following in vitro zoospore binding assays. Moreover, Pro73Gly particles are transmitted only poorly by O.bornovanus. Together, the results of these studies suggest that CNV undergoes conformational change upon zoospore binding and that the conformational change is important for CNV transmissibility.
authors.affiliation: Faculty of Agricultural Sciences, University of British Columbia, Vancouver, BC, Canada V6T 1Z4.
authors.forename: Kishore
authors.initials: K
authors.lastname: Kakani
authors.forename: Ron
authors.initials: R
authors.lastname: Reade
authors.forename: D'Ann
authors.initials: D
authors.lastname: Rochon
mesh_terms: D018553:Cucumis sativus / Q000382:microbiology* / Q000821:virology*; D005658:Fungi / Q000378:metabolism / Q000821:virology*; D009154:Mutation; D010942:Plant Viruses / Q000235:genetics / Q000502:physiology*; D011487:Protein Conformation; D013172:Spores, Fungal / Q000378:metabolism / Q000821:virology; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014771:Virion / Q000378:metabolism; D014777:Virus Diseases / Q000378:metabolism / Q000635:transmission*
chemical_list: D014764:Viral Proteins