title: Silicon chip with capacitors and transistors for interfacing organotypic brain slice of rat hippocampus.
abstract: Probing projections between brain areas and their modulation by synaptic potentiation requires dense arrays of contacts for noninvasive electrical stimulation and recording. Semiconductor technology is able to provide planar arrays with high spatial resolution to be used with planar neuronal structures such as organotypic brain slices. To address basic methodical issues we developed a silicon chip with simple arrays of insulated capacitors and field-effect transistors for stimulation of neuronal activity and recording of evoked field potentials. Brain slices from rat hippocampus were cultured on that substrate. We achieved local stimulation of the CA3 region by applying defined voltage pulses to the chip capacitors. Recording of resulting local field potentials in the CA1 region was accomplished with transistors. The relationship between stimulation and recording was rationalized by a sheet conductor model. By combining a row of capacitors with a row of transistors we determined a simple stimulus-response matrix from CA3 to CA1. Possible contributions of inhomogeneities of synaptic projection, of tissue structure and of neuroelectronic interfacing were considered. The study provides the basis for a development of semiconductor chips with high spatial resolution that are required for long-term studies of topographic mapping.
authors.affiliation: Department of Membrane and Neurophysics, Max Planck Institute for Biochemistry, Martinsried/München 82152, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Hutzler
authors.forename: Peter
authors.initials: P
authors.lastname: Fromherz
mesh_terms: D000818:Animals; D025802:Electric Capacitance; D004558:Electric Stimulation / Q000379:methods; D006624:Hippocampus / Q000502:physiology*; D008832:Microchemistry / Q000295:instrumentation* / Q000379:methods; D009924:Organ Culture Techniques / Q000295:instrumentation / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D012666:Semiconductors; D012825:Silicon; D009435:Synaptic Transmission / Q000502:physiology*; D014173:Transistors, Electronic
chemical_list: D012825:Silicon