title: [Cloning and sequencing of cathepsin L1 (FheCL1) gene cDNA of Fasciola hepatica].
abstract: OBJECTIVE
  To search for a candidate DNA vaccine of Fasciola hepatica.
  
  
  METHODS
  Using RT-PCR and digestion with Hind III and BamHI, Fasciola hepatica secreted cathepsin L1 (FheCL1) cDNA was cloned into the expression vector pcDNA3.1.
  
  
  RESULTS
  The cloning was successful, the cDNA sequence and its deduced amino acid sequence were analyzed. There was much difference between the cloned FheCL1 and the published one. But the first 20 residues of their amino acid sequences were the same.
  
  
  CONCLUSIONS
  The recombinant plasmid pcDNA3.1-FheCL1 may be a new type of candidate DNA vaccine candidate for Fasciola hepatica. It is possible that Fasciola hepatica presents different sub-species but their amino acid residues (1 to 20) encoded by FheCL1 might build up membrane spanning helix.
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642.
authors.forename: Ren
authors.initials: R
authors.lastname: Zhang
authors.forename: Hai-yun
authors.initials: HY
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D056668:Cathepsin L; D002403:Cathepsins / Q000235:genetics*; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases; D018076:DNA, Complementary / Q000737:chemistry; D005210:Fasciola hepatica / Q000201:enzymology* / Q000235:genetics* / Q000276:immunology; D017238:Genes, Helminth; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019444:Vaccines, DNA
chemical_list: D018076:DNA, Complementary; D019444:Vaccines, DNA; D002403:Cathepsins; D003546:Cysteine Endopeptidases; D056668:Cathepsin L