Quantitative magnetic resonance imaging of urea and lysozyme in protein chromatography.
Magnetic resonance imaging (MRI) techniques have been implemented to enable quantitative imaging of protein and urea within a 5 ml HiTrap size-exclusion chromatography desalting column, without introduction of contrast agents. One-, two- and three-dimensional images of urea injected at concentrations of 2, 4, 6 and 8 M were acquired. One-dimensional profiles of lysozyme at concentrations between 5 and 25 mg ml(-1) were also obtained. All data were accurate to within +/- 15% when compared to the known amount injected. Quantitative MRI elution profiles of both urea and lysozyme were then obtained in real-time during a desalting separation.
Holland
D J
DJ
Department of Chemical Engineering, University of Cambridge, Pembroke Street, Cambridge CB2 3RA, UK.
Sederman
A J
AJ
Mantle
M D
MD
Gladden
L F
LF
Middelberg
A P J
AP
D008279:Magnetic Resonance Imaging / Q000379:methods*; D009113:Muramidase / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D014508:Urea / Q000737:chemistry*
D011506:Proteins; D014508:Urea; D009113:Muramidase