Monoamines increase the excitability of spinal neurones in the neonatal rat by hyperpolarizing the threshold for action potential production.
During fictive locomotion in the adult decerebrate cat, motoneurone excitability is increased by a hyperpolarization of the threshold potential at which an action potential is elicited (V(th)). This lowering of V(th) occurs at the onset of fictive locomotion, is evident for the first action potential elicited and is presumably caused by a neuromodulatory process. The present study tests the hypothesis that the monoamines serotonin (5-HT) and noradrenaline (NA) can hyperpolarize neuronal V(th). The neonatal rat isolated spinal cord preparation and whole-cell recording techniques were used to examine the effects of bath-applied 5-HT and NA on the V(th) of spinal ventral horn neurones. In the majority of lumbar ventral horn neurones, 5-HT (13/26) and NA (10/16) induced a hyperpolarization of V(th) ranging from -2 to -8 mV. 5-HT and NA had similar effects on V(th) for individual neurones. This hyperpolarization of V(th) was not due to a reduction of an accommodative process, and could be seen without changes in membrane potential or membrane resistance. These data reveal a previously unknown action of 5-HT and NA, hyperpolarization of V(th) of spinal neurones, a process that would facilitate both neuronal recruitment and firing.
Fedirchuk
Brent
B
Department of Physiology, University of Manitoba, 730 William Avenue, Winnipeg, MB, Canada R3E 3J7. brent@scrc.umanitoba.ca
Dai
Yue
Y
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D000831:Animals, Newborn; D000870:Anterior Horn Cells / Q000187:drug effects* / Q000502:physiology; D066298:In Vitro Techniques; D008124:Locomotion / Q000502:physiology; D009638:Norepinephrine / Q000494:pharmacology*; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000494:pharmacology*; D013116:Spinal Cord / Q000187:drug effects* / Q000502:physiology
D012701:Serotonin; D009638:Norepinephrine