Risk factors for periodontitis in HIV patients.
OBJECTIVE
The purpose of this study was to identify risk factors for periodontitis associated with human immunodeficiency virus (HIV) infection.


METHODS
A total of 152 HIV(+) patients were recruited from the CARE clinic at the University of the Pacific School of Dentistry. Clinical measurements (gingival index, plaque index, bleeding index, probing depth, and attachment loss), gingival crevicular fluid (GCF) and subgingival plaque samples were taken from eight sites of each patient at baseline and 6-month visits. GCF neutrophil elastase was determined by measurement of p-nitroanalide resulting from hydrolysis of an elastase-specific peptide. GCF beta-glucuronidase was determined by release of 4-methylumbelliferone from hydrolysis of a specific substrate. A bacterial concentration fluorescence immunoassay was used to detect periodontopathic bacteria in subgingival plaque samples.


RESULTS
Viral load, age, smoking pack-years, Fusobacterium nucleatum, Prevotella intermedia, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, neutrophil elastase, and beta-glucuronidase were significantly correlated with clinical measurements (0.0001 < p < 0.05). Significantly higher levels of elastase, beta-glucuronidase, F. nucleatum, P. intermedia, and A. actinomycetemcomitans were found at progressing sites than in non-progressing sites (0.001 < p < 0.05).


CONCLUSIONS
These data indicate that age, smoking pack-years, viral load, F. nucleatum, P. intermedia, A. actinomycetemcomitans, elastase, and beta-glucuronidase are risk factors for periodontitis in HIV(+) patients.
Alpagot
Tamer
T
University of the Pacific School of Dentistry, San Francisco, CA 94115, USA. talpagot@pacific.edu
Duzgunes
Nejat
N
Wolff
Larry F
LF
Lee
Aaaron
A
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000704:Analysis of Variance; D001421:Bacteria, Anaerobic / Q000472:pathogenicity; D003773:Dental Plaque / Q000382:microbiology; D005883:Gingival Crevicular Fluid / Q000201:enzymology; D005966:Glucuronidase / Q000032:analysis; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D019272:Leukocyte Elastase / Q000032:analysis; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D010518:Periodontitis / Q000209:etiology*; D012044:Regression Analysis; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects; D018709:Statistics, Nonparametric; D019562:Viral Load
D005966:Glucuronidase; D019272:Leukocyte Elastase