Nuclear localization and interaction of RolB with plant 14-3-3 proteins correlates with induction of adventitious roots by the oncogene rolB.
The rooting-locus gene B (rolB) on the T-DNA of the root-inducing (Ri) plasmid in Agrobacterium rhizogenes is responsible for the induction of transformed adventitious roots, although the root induction mechanism is unknown. We report here that the RolB protein of pRi1724 (1724RolB) is associated with Nicotianatabacum14-3-3-like protein omegaII (Nt14-3-3 omegaII) in tobacco bright yellow (BY)-2 cells. Nt14-3-3 omegaII directly interacts with 1724RolB protein. Green fluorescent protein (GFP)-fused 1724RolB is localized to the nucleus. GFP-fused mutant 1724RolB proteins having a deletion or amino acid substitution are unable to interact with Nt14-3-3 omegaII and also show impaired nuclear localization. Moreover, these 1724RolB mutants show decreased capacity for adventitious root induction. These results suggest that adventitious root induction by 1724RolB protein correlates with its interaction with Nt14-3-3 omegaII and the nuclear localization of 1724RolB protein.
Moriuchi
Hiroshi
H
Center for Gene Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8527, Japan.
Okamoto
Chiho
C
Nishihama
Ryuichi
R
Yamashita
Ichiro
I
Machida
Yasunori
Y
Tanaka
Nobukazu
N
D048948:14-3-3 Proteins; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics; D011485:Protein Binding; D012231:Rhizobium / Q000235:genetics / Q000472:pathogenicity; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics / Q000378:metabolism*; D001617:beta-Glucosidase / Q000235:genetics* / Q000378:metabolism*
D048948:14-3-3 Proteins; D001426:Bacterial Proteins; D010940:Plant Proteins; D014446:Tyrosine 3-Monooxygenase; C092241:RolB protein, Agrobacterium rhizogenes; D001617:beta-Glucosidase