Determination of protein-ligand association thermochemistry using variable-temperature nanoelectrospray mass spectrometry.
A novel temperature-controlled nanoelectrospray (nanoES) device, interfaced with a Fourier transform ion cyclotron resonance mass spectrometer, is used to measure the association constants, Kassoc, for a series of protein-carbohydrate complexes at solution temperatures ranging from 5 to 40 degrees C. From a van't Hoff analysis of the Kassoc values, the enthalpies and entropies of association (DeltaHassoc, DeltaSassoc) at 25 degrees C are determined. The nanoES/mass spectrometry-derived thermodynamic parameters are in agreement with values previously determined by isothermal titration calorimetry.
Daneshfar
Rambod
R
Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2G2.
Kitova
Elena N
EN
Klassen
John S
JS
D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D007128:Immunoglobulin Fragments / Q000737:chemistry / Q000378:metabolism*; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism*; D007135:Immunoglobulin Variable Region / Q000737:chemistry / Q000378:metabolism; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D013816:Thermodynamics
D002241:Carbohydrates; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D007135:Immunoglobulin Variable Region