title: The molecular scanner: concept and developments.
abstract: Approaches aimed at deciphering the proteome have illustrated the need for relatively complex and highly sensitive methodologies. The major elements of proteome analysis, such as powerful protein separation and enzymatic processing, mass spectrometry and dedicated bioinformatics have been assembled in the development of the molecular scanner. This highly flexible and data-rich approach has combined the power of electrophoretic protein separation, the simultaneous digestion and transfer of proteins through an enzymatic membrane, the immediate use of the MALDI mass spectrometer to scan a collecting membrane, and the development of dedicated bioinformatics tools to perform protein identification and molecular imaging of the proteome. Clinical applications of the molecular scanner have also started to be developed for disease diagnosis in biological material.
authors.affiliation: Swiss Institute of Bioinformatics, Proteome Informatics Group, CMU, Michel Servet 1, 1211 Geneva, Switzerland. Pierre-Alain.Binz@isb-sib.ch
authors.forename: Pierre-Alain
authors.initials: PA
authors.lastname: Binz
authors.forename: Markus
authors.initials: M
authors.lastname: Müller
authors.forename: Christine
authors.initials: C
authors.lastname: Hoogland
authors.forename: Catherine
authors.initials: C
authors.lastname: Zimmermann
authors.forename: Carla
authors.initials: C
authors.lastname: Pasquarello
authors.forename: Garry
authors.initials: G
authors.lastname: Corthals
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Sanchez
authors.forename: Denis F
authors.initials: DF
authors.lastname: Hochstrasser
authors.forename: Ron D
authors.initials: RD
authors.lastname: Appel
mesh_terms: D000818:Animals; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods / Q000639:trends; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D006801:Humans; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D040901:Proteomics / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D020539:Sequence Analysis, Protein / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D018511:Systems Integration; D014584:User-Computer Interface
chemical_list: D011506:Proteins