Separation and screening of compounds of biological origin using molecularly imprinted polymers.
Molecularly imprinted polymers (MIPs) represent a new class of materials possessing high selectivity and affinity for the target molecule. Since their discovery in 1972, molecularly imprinted polymers have attracted considerable interest from bio- and chemical laboratories to pharmaceutical institutes. They have been utilized as sensors, catalysts, sorbents for solid-phase extraction, stationary phase for liquid chromatography, mimics of enzymes, receptors and antibodies. Among which, the application of molecularly imprinted polymers for molecular recognition-based separation and screening of compounds has undergone rapid extension and received much attention in recent years. This article mainly focuses on the separation and screening of certain pharmacophoric compounds of interests from biological origin using molecular imprinting technology. Examples of extraction and recognition of active components as anti-tumors or anti-Hepatitis C virus inhibitors from Chinese traditional herbs using molecularly imprinting technology are particularized in this article. Comparison between the screening effect based on MIPs and that based on antibodies is also represented. Consequently the merits and demerits of these two technologies are highlighted.
Xu
Xiaojie
X
College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. xiaojxu@chem.pku.edu.cn
Zhu
Lili
L
Chen
Lirong
L
D001688:Biological Products / Q000032:analysis* / Q000302:isolation & purification; D002853:Chromatography, Liquid; D011108:Polymers / Q000737:chemistry*; D012680:Sensitivity and Specificity
D001688:Biological Products; D011108:Polymers