title: An experimental study on the features of peri-implant epithelium: immunohistochemical and electron-microscopic observations.
abstract: The purpose of this study was to investigate the immunohistochemical and the ultrastructural features of the implant circumference epithelium of the beagle dog using various types of antibodies. The peri-implant epithelium was at an acute-angle from the gingival epithelium and was arranged in parallel to the implant surface. With immunohistochemical staining, the peri-implant epithelium was strongly positive for KL-1, and weakly positive for CK4, CK8 and CK19. These positive reactions for keratins and also for PCNA and BM-1 were similar to those seen in the oral mucosa. In the peri-implant epithelium, a plentitude of microvilli were observed at the periphery of cells at the implant sites, and bacteria were observed between the implant and the peri-implant epithelium without the formation of half desmosomes. There were many lipid-like vacuoles or lysosome-like granules. The intercellular space was wider than the junctional epithelium, and random migrations of large numbers of neutrophils could be seen. Taken together, the peri-implant epithelium is similar to that seen in the oral mucosa, and it is structurally different from the junctional epithelium.
authors.affiliation: Oral Health Science Center, Department of Pathology, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba 261-8502, Japan.
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Fujiseki
authors.forename: Kenichi
authors.initials: K
authors.lastname: Matsuzaka
authors.forename: Masao
authors.initials: M
authors.lastname: Yoshinari
authors.forename: Masaki
authors.initials: M
authors.lastname: Shimono
authors.forename: Takashi
authors.initials: T
authors.lastname: Inoue
mesh_terms: D000818:Animals; D015921:Dental Implants; D004285:Dogs; D004846:Epithelial Attachment / Q000166:cytology*; D004847:Epithelial Cells / Q000737:chemistry* / Q000648:ultrastructure*; D005260:Female; D022002:Hemidesmosomes / Q000648:ultrastructure; D007124:Immunoenzyme Techniques; D020007:Implants, Experimental; D007633:Keratins / Q000032:analysis; D008854:Microscopy, Electron; D008871:Microvilli / Q000648:ultrastructure; D009061:Mouth Mucosa / Q000166:cytology*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011506:Proteins / Q000032:analysis
chemical_list: D015921:Dental Implants; D018809:Proliferating Cell Nuclear Antigen; D011506:Proteins; C071041:BM-1, platelet aggregation inhibitor; D007633:Keratins