Vectors for RNAi technology in poplar.
The potential of double-stranded RNA interference (RNAi) technology was studied for down-regulation of gene expression in poplar. A set of vectors was constructed generating RNAs capable of duplex formation of sequences specific for the beta-glucuronidase (GUS) reporter gene system. These gene cassettes are driven by the CaMV-35S promoter. To address the question of gene silencing, we tested the functionality of these vectors, both in transient assays by transforming protoplasts with the RNAi constructs, and in stably transformed GUSexpressing poplar plants. Agrobacterium-mediated transformation of those GUS-expressing plants with a GUS-specific RNAi construct showed a strong down-regulation of the reporter gene. From these results we conclude that RNAi is also functional in poplar.
Meyer
S
S
Institut für Pflanzenbiologie, Technische Universität Braunschweig, Braunschweig, Germany.
Nowak
K
K
Sharma
V K
VK
Schulze
J
J
Mendel
R R
RR
Hänsch
R
R
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D017930:Genes, Reporter; D005822:Genetic Vectors; D005966:Glucuronidase / Q000235:genetics; D030821:Plants, Genetically Modified; D032107:Populus / Q000235:genetics*; D034622:RNA Interference; D011994:Recombinant Proteins / Q000235:genetics; D012231:Rhizobium / Q000235:genetics; D014170:Transformation, Genetic
D017931:DNA Primers; D011994:Recombinant Proteins; D005966:Glucuronidase