Alteration of urothelial-mediated tone in the ischemic bladder: role of eicosanoids.
OBJECTIVE
Previously we showed that ischemia alters bladder smooth muscle contractility in the rabbit. This study investigates the role of urothelium and eicosanoid-release in ischemic bladder smooth muscle instability.


METHODS
Male New Zealand white rabbits were divided into treated (n = 12) and age-matched control (n = 10) groups. The treated group underwent balloon endothelial injury of the iliac arteries, and then received 4 weeks of cholesterol diet, followed by 4 weeks of regular diet. The control group received a regular diet for 8 weeks. After 8 weeks, blood flow for both the iliac arteries and the bladder as well as bladder oxygen tension were recorded. In one-half of each ischemic and control bladder, the urothelium was removed. Bladder tissues were processed for organ bath and enzyme-immunoassay (EIA) of prostaglandins (PGs) and leukotrienes (LTs).


RESULTS
A significant decrease in iliac arterial blood flow, bladder wall blood flow, and bladder oxygen tension was found in the treated group. Bladder ischemia increased the frequency and amplitude of baseline spontaneous smooth muscle contractility. Ischemic tissues with urothelium (Uro+) demonstrated significant increases in the contractile response to electrical field stimulation (EFS) and carbachol relative to control Uro+ tissues. Urothelial removal increased smooth muscle contraction in the control tissues but had no significant effect in the ischemic/hypoxic tissues. Contraction of control tissues without urothelium (Uro-) was similar to contraction of ischemic Uro+ tissues. Contractions of ischemic Uro+ and control Uro- tissues were unchanged after treatment with the cyclooxygenase (COX) inhibitor indomethacin, while they were significantly reduced by the 5-lipoxygenase (5-LO) inhibitor NDGA. EIA showed no change in PGs release from the ischemic urothelium, but significant increase in PGF(2-alpha) and thromboxane A(2) release from the ischemic suburothelial tissue. Ischemia increased the release of LTB(4), LTC(4), and LTE(4) from both urothelium and suburothelial tissue.


CONCLUSIONS
Our studies suggest loss of urothelial-mediated tone and LTs-mediated smooth muscle instability in the chronically ischemic/hypoxic bladder.
Azadzoi
Kazem M
KM
Urology Research (151), Boston University School of Medicine and VA Boston Medical Center, 150 South Huntington Avenue, Boston, MA 02130, USA. kazadzoi@bu.edu
Heim
Vanessa K
VK
Tarcan
Tufan
T
Siroky
Mike B
MB
D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000502:physiology; D001794:Blood Pressure / Q000502:physiology; D002217:Carbachol / Q000494:pharmacology; D015777:Eicosanoids / Q000378:metabolism / Q000502:physiology*; D004558:Electric Stimulation; D007083:Iliac Artery / Q000502:physiology; D007124:Immunoenzyme Techniques; D007511:Ischemia / Q000503:physiopathology*; D007537:Isometric Contraction / Q000502:physiology; D008297:Male; D018721:Muscarinic Agonists / Q000494:pharmacology; D009129:Muscle Tonus / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000502:physiology; D010100:Oxygen / Q000097:blood; D011451:Prostaglandin-Endoperoxide Synthases / Q000502:physiology; D011817:Rabbits; D001743:Urinary Bladder / Q000098:blood supply* / Q000378:metabolism / Q000503:physiopathology
D015777:Eicosanoids; D018721:Muscarinic Agonists; D002217:Carbachol; D001094:Arachidonate 5-Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases; D010100:Oxygen