[Angiotensin II-induced podocyte apoptosis: role of the MAPK subtypes].
OBJECTIVE
To evaluate the role of the MAPK subtypes (p38MAPK, ERK and JNK) in ANG II-induced apoptosis of cultured human podocytes.


METHODS
The cultured podocytes were incubated in media containing either vehicle, SB202190(5 micromol/L, an inhibitor of p38MAPK), PD98059 (1 micromol/L, an inhibitor of ERK), SP600125 (5 micromol/L, an inhibitor of JNK), ANG II (10(-8)mol/L) with or without SB202190 PD98059 and SP600125 for 18 hours; the cells were assayed for apoptosis by morphologic staining with H-33342 and propidium iodide and DNA fragmentation assays; the cell proteins were probed for phosphorylated MAPKs to determine the activation of specific MAPK subtypes.


RESULTS
ANG II promoted podocyte apoptosis in a time- and dose-dependent manner; ANG II stimulated p38MAPK, but inhibited JNK; SB202190 inhibited both ANG II-induced podocyte apoptosis and p38MAPK phosphorylation; Inhibition of ERK by PD98059 had no effect on ANG II-induced cell apoptosis.


CONCLUSIONS
ANG II-induced apoptosis through stimulation of p38MAPK and inhibition of JNK in human podocytes.
Lai
Xiao-xi
XX
Department of Nephrology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
Ding
Guo-hua
GH
Huang
Cong-xin
CX
Shi
Ming
M
Chen
Cheng
C
D000804:Angiotensin II / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053938:DNA Fragmentation; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases; D007678:Kidney Glomerulus / Q000166:cytology / Q000187:drug effects*; D020928:Mitogen-Activated Protein Kinases / Q000502:physiology*; D048051:p38 Mitogen-Activated Protein Kinases
D000804:Angiotensin II; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases