Identification of specific peptide ligands for B-lymphoma cell and its effect on tyrosine phosphorylation and cell apoptosis.
OBJECTIVE
To search novel method for diagnosis and therapy of B-lymphoma, specific small molecular peptide ligands against binding site of tumor cells were screened and its effects on signal transduction and cell apoptosis were tested.


METHODS
Specific peptide ligands were screened by binding with site of human B lymphoma cell (OC1LY8) using peptide-bead libraries. The identified peptides were characterized with responsible cells by rebinding test. The role of tyrosine phosphorylation of peptide ligand was tested by Western blot; and its apoptosispromoting role was observed by confocal fluorescent microscope.


RESULTS
Specific peptide ligand was able to bind specifically to site on cell surface and enter into cytoplasm. Tetrameric peptide ligand was able to strongly trigger signal transduction resulting in tyrosine phosphorylation and cellular apoptosis in OC1LY8 cell line.


CONCLUSIONS
Screened peptide ligand can effectively bind with OC1LY8 cell, stimulate cellular tyrosine phosphorylation and induce cellular apoptosis.
Song
Liangwen
L
Department of Pathology, Beijing Institute of Radiation Medicine, Beijing 100850.
Ma
Xianmei
X
Cui
Xuemei
X
Li
Yang
Y
Wang
Xiaomin
X
D000595:Amino Acid Sequence; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D006801:Humans; D008024:Ligands; D016393:Lymphoma, B-Cell / Q000378:metabolism / Q000473:pathology; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D019151:Peptide Library; D010766:Phosphorylation / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects*; D014443:Tyrosine / Q000378:metabolism*
D008024:Ligands; D009842:Oligopeptides; D019151:Peptide Library; D014443:Tyrosine