Semaphorin and neuropilin expression in motoneurons after intraspinal motoneuron axotomy.
We have examined mRNA and protein distribution for the axon guidance molecules semaphorin3A, 3F, 4F and semaphorin receptors neuropilin-1 and 2, 1-21 days after intramedullary axotomy of rat lumbar spinal cord motoneurons. We show that semaphorin3A mRNA and protein are up-regulated in the scar and in motoneurons from 3 days and upto 3 weeks after injury. Neuropilin-1 mRNA showed no changed expression in axotomized motoneurons. Semaphorin3F mRNA expression was found in ventral roots after ventral funiculus lesion (VFL) and neuropilin-2 mRNA was found in affected motoneurons from 1 day after injury throughout the examined period. Semaphorin4F mRNA was first found in motoneurons 3 weeks after lesion. These results suggest semaphorin/neuropilin involvement in the injury response of intramedullary axotomized motoneurons.
Lindholm
T
T
Swedish Defence Research Agency, Experimental Traumatology, Stockholm, Sweden.
Sköld
M K
MK
Suneson
A
A
Carlstedt
T
T
Cullheim
S
S
Risling
M
M
D000818:Animals; D019771:Axotomy; D004195:Disease Models, Animal; D005260:Female; D020439:Growth Cones / Q000378:metabolism* / Q000648:ultrastructure; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009046:Motor Neurons / Q000166:cytology / Q000378:metabolism*; D009416:Nerve Regeneration / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D039942:Neuropilin-1 / Q000235:genetics / Q000378:metabolism; D039943:Neuropilin-2 / Q000235:genetics / Q000378:metabolism; D039921:Neuropilins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D040121:Semaphorin-3A / Q000235:genetics / Q000378:metabolism; D039961:Semaphorins / Q000235:genetics / Q000378:metabolism*; D013119:Spinal Cord Injuries / Q000503:physiopathology / Q000628:therapy; D013126:Spinal Nerve Roots / Q000166:cytology / Q000254:growth & development* / Q000293:injuries; D015854:Up-Regulation / Q000235:genetics
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D039943:Neuropilin-2; D039921:Neuropilins; D012333:RNA, Messenger; C491229:Sema3f protein, mouse; D040121:Semaphorin-3A; D039961:Semaphorins; D039942:Neuropilin-1