Characterization of a new HpaI centromeric satellite DNA in Salmo salar.
A highly repeated HpaI DNA family was revealed in Atlantic salmon (Salmo salar) and analyzed by Southern blotting and fluorescence in situ hybridization (FISH). In this report, we describe the nucleotide sequence, genomic structure and chromosomal localization of this HpaI repeat. This novel satellite appeared tandemly arrayed and located at centromeric areas of three acrocentric chromosome pairs as evidenced by FISH. The sequence was characterized by a high AT content (63%), a short consensus motif (A/T)(G/C)AAA(T/C) similar to other centromeric satellites motifs, and by short AT enriched stretches. The presence of this sequence in other salmonid species was also tested by Southern blot hybridization and used to analyze its evolution within this group.
Viñas
Ana
A
Departamento de Genética, Facultad de Biología, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain. avinas@lugo.usc.es
Abuín
María
M
Pardo
Belén G
BG
Martínez
Paulino
P
Sánchez
Laura
L
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002503:Centromere; D004276:DNA, Satellite / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D020317:Salmo salar / Q000235:genetics*
D004276:DNA, Satellite