Blue light-absorbing intraocular lens and retinal pigment epithelium protection in vitro.
OBJECTIVE
To compare the Alcon AcrySof Natural (SN60AT) and AcrySof (SA60AT), the AMO Sensar (AR40e) and ClariFlex, and the Pfizer CeeOn Edge 911A intraocular lenses (IOLs) as to their ability to protect retinal pigment epithelial (RPE) cells from light damage mediated by the lipofuscin fluorophore A2E.


METHODS
Department of Ophthalmology, Columbia University, New York, New York, USA.


METHODS
Cultured human RPE cells (ARPE-19 cell line) that had accumulated A2E were exposed to blue (430 nm +/- 30), green (550 +/- 10 nm), or white (390 to 750 nm) light with and without an IOL in the light path.


RESULTS
The blue light-absorbing AcrySof Natural IOL was associated with significant reduction (78% to 82%; P<.01) in the death of A2E-laden RPE that were exposed to blue, white, and green light. The decrease in the incidence of cell death was greater in magnitude than would be expected from the amount of light that was absorbed by the IOL. The considerably smaller declines in cell death observed with the AcrySof, Sensar, ClariFlex, and CeeOn Edge IOLs were likely due to nonspecific reductions in light transmittance.


CONCLUSIONS
By absorbing blue light, the AcrySof Natural IOL shields RPE cells that have accumulated the aging lipofuscin fluorophore A2E from the damaging effects of light. A long-term population-based clinical trial would determine whether a blue light-absorbing IOL can reduce the risk for or progression of age-related macular degeneration.
Sparrow
Janet R
JR
Department of Ophthalmology, Columbia University, 630 West 128th Street, New York, NY 10032, USA. jrs88@columbia.edu
Miller
Ashley S
AS
Zhou
Jilin
J
D000042:Absorption; D017209:Apoptosis / Q000528:radiation effects; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D019610:Cytoprotection; D006801:Humans; D007910:Lenses, Intraocular; D008027:Light; D010857:Pigment Epithelium of Eye / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects*; D011474:Prosthesis Design; D011726:Pyridinium Compounds / Q000378:metabolism; D011835:Radiation Protection / Q000295:instrumentation*; D012176:Retinoids / Q000378:metabolism
C112040:A2-E (N-retinylidene-N-retinylethanolamine); D011726:Pyridinium Compounds; D012176:Retinoids