Traditional Korean medicine (SCRT) modulate Th1/Th2 specific cytokine production in mice CD4+ T cell.
Traditional Korea medicine, So-Cheong-Ryong-Tang (SCRT) also called as Xiao-qing-long-tang or Sho-seiru-to, contains eight species of medicinal plants and has been used for treating allergic diseases, such as allergic rhinitis and asthma, for hundreds of years in Asian countries. CD4+ T cells were highly purified by using magnetic bead from splenocytes of BALB/c mice. SCRT treatment slightly decreased the expression of cell surface protein CD69 on CD4+ T cell in the flow cytometry analysis. In RT-PCR analysis, SCRT increases the expression of IL-2 and IL2R-alpha mRNA, and decreases the expression of IL-4 mRNA, which is an important cytokine of Th2 cell development. On the other hand, SCRT treatment increases IFN-gamma expression, which is one of the key cytokines for Th1 cell development. Present study implies that SCRT can correct Th2 dominant condition directly affecting to the CD4+ T cell without significantly depressing general T cell activities. These results also suggest that the effect on CD4+ T cell may be the one of key pharmacological effect point for treating IgE medicated allergic asthma by SCRT.
Ko
Eunjung
E
Department of Physiology, College of Oriental Medicine, Kyunghee University, #1 Heoki-dong Dongdaemoon-gu, Seoul 130-701, South Korea.
Rho
Samwoong
S
Lee
Eui-Joon
EJ
Seo
Young-Ho
YH
Cho
Chongwoon
C
Lee
Yongwon
Y
Min
Byung-Il
BI
Shin
Min-Kyu
MK
Hong
Moo-Chang
MC
Bae
Hyunsu
H
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis*; D017931:DNA Primers; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D005434:Flow Cytometry; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D007723:Korea; D008519:Medicine, Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D008517:Phytotherapy; D010946:Plants, Medicinal; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018417:Th1 Cells / Q000187:drug effects / Q000378:metabolism; D018418:Th2 Cells / Q000187:drug effects / Q000378:metabolism
D000276:Adjuvants, Immunologic; D016207:Cytokines; D017931:DNA Primers; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; C090832:sho-seiryu-to; D015847:Interleukin-4; D007371:Interferon-gamma