Over-expression of the testis-specific gene TSGA10 in cancers and its immunogenicity.
The TSGA10 gene was originally isolated in normal testis by differential mRNA display. TSGA10 is located on chromosome 2q11.2 and consists of 19 exons extending over 3 kb. TSGA10 mRNA expression was investigated in normal and malignant tissues using quantitative real-time RT-PCR. It was predominantly expressed in the testis in adult normal tissues. In malignant tissues, TSGA10 was over-expressed in 4 of 20 hepatocellular carcinomas (HCC), 1 of 20 colon cancers, 7 of 20 ovarian cancers, 3 of 20 prostate cancers, 1 of 21 malignant melanomas, and 8 of 21 bladder cancers. Serological analysis revealed that 3 out of 346 patients with various types of cancer possessed antibody against recombinant TSGA10 protein. They included 2 patients with hepatocellular carcinoma and a patient with malignant melanoma.
Tanaka
Ryo
R
Departments of Immunology, Okayama University Graduate School of Medicine and Dentistry, Japan. meneki@md.okayama-u.ac.jp
Ono
Toshiro
T
Sato
Shuichiro
S
Nakada
Tetsuya
T
Koizumi
Fumihito
F
Hasegawa
Kosei
K
Nakagawa
Kazuhiko
K
Okumura
Hideo
H
Yamashita
Toshiharu
T
Ohtsuka
Mikio
M
Asagoe
Kenji
K
Yamasaki
Osamu
O
Noguchi
Yuji
Y
Iwatsuki
Keiji
K
Nakayama
Eiichi
E
D000906:Antibodies / Q000097:blood; D003598:Cytoskeletal Proteins; D006801:Humans; D008297:Male; D009369:Neoplasms / Q000276:immunology / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis; D015854:Up-Regulation
D000906:Antibodies; D003598:Cytoskeletal Proteins; D011506:Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; C429756:TSGA10 protein, human