Synergistic temperature and ethanol effect on Saccharomyces cerevisiae dynamic behaviour in ethanol bio-fuel production.
The impact of ethanol and temperature on the dynamic behaviour of Saccharomyces cerevisiae in ethanol biofuel production was studied using an isothermal fed-batch process at five different temperatures. Fermentation parameters and kinetics were quantified. The best performances were found at 30 and 33 degrees C around 120 g l(-1) ethanol produced in 30 h with a slight benefit for growth at 30 degrees C and for ethanol production at 33 degrees C. Glycerol formation, enhanced with increasing temperatures, was coupled with growth for all fermentations; whereas, a decoupling phenomenon occurred at 36 and 39 degrees C pointing out a possible role of glycerol in yeast thermal protection.
Aldiguier
A S
AS
Institut National des Sciences Appliquées, Département de Génie Biochimique et Alimentaire, 135 Avenue de Rangueil, 31077 Toulouse Cedex, France.
Alfenore
S
S
Cameleyre
X
X
Goma
G
G
Uribelarrea
J L
JL
Guillouet
S E
SE
Molina-Jouve
C
C
D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D000431:Ethanol / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D013696:Temperature
D000431:Ethanol; D005990:Glycerol