Obese individuals as thiamin storers.
OBJECTIVE
To investigate thiamin and its phosphoester content in plasma and erythrocytes for a complete picture of thiamin status in obese individuals.


METHODS
Comparative study of the thiamin status of obese vs normal individuals.


METHODS
In all, 10 healthy, overweight, fertile age women (age: 33.1+/-5.1 y; BMI: 47.0+/-0.2 kg/m(2)) and 10 normal women (age: 30.1+/-3.5 y; BMI: 22.8+/-0.2 kg/m(2)).


METHODS
a high-pressure liquid chromatography (HPLC) method for the determination of thiamin and its phosphoesters in the plasma and erythrocytes of the subjects.


RESULTS
The major findings were: (1) significant decrease of plasma thiamin, its monophosphate and total thiamin contents in obese vs normal women; (2) significant decrease of thiamin pyrophosphate ester and total thiamin content in obese vs normal women; (3) significant increase in plasma thiamin/thiamin monophosphate ratio (in practice, it was inverted) and corresponding decrease of the plasma thiamin monophosphate/erythrocytes thiamin pyrophosphate ratio in obese vs normal women, where plasma thiamin monophosphate and erythrocytes thiamin pyrophosphate contents are an index of thiamin status.


CONCLUSIONS
This study advances the hypothesis that obese women maintain higher levels of thiamin compared to normal weight subjects by storing greater amounts of thiamin in cells through preferential intracellular thiamin recycling to compensate for relatively lower levels of thiamin.
Patrini
C
C
Department of Experimental Medicine, Section for Human Physiology, University of Pavia, Pavia, Italy. cpatrini@unipv.it
Griziotti
A
A
Ricciardi
L
L
D000328:Adult; D000886:Anthropometry; D001800:Blood Specimen Collection / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D006801:Humans; D009765:Obesity / Q000097:blood*; D013831:Thiamine / Q000097:blood*; D013833:Thiamine Monophosphate / Q000097:blood; D013835:Thiamine Pyrophosphate / Q000097:blood
D013833:Thiamine Monophosphate; D013835:Thiamine Pyrophosphate; D013831:Thiamine