Crystallization and preliminary crystallographic analysis of BbCRASP-1, a complement regulator-acquiring surface protein of Borrelia burgdorferi.
Borrelia burgdorferi is the causative agent of Lyme disease. Serum-resistant strains of the pathogen are able to reduce the host's immune response to infection by recruiting fluid-phase complement regulators from the serum. B. burgdorferi complement regulator-acquiring surface protein-1 (BbCRASP-1) binds factor H and factor-H-like protein-1 to the bacterial surface, where they actively down-regulate complement response. Crystals of native and selenomethionine-substituted BbCRASP-1 have been obtained and a native data set to 2.7 A as well as selenomethionine MAD data to 3.2 A resolution have been collected. The selenium substructure has been solved and initial phases have been refined to 3.0 A by density-modification methods. Model building and refinement are under way.
Cordes
Frank S
FS
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, England.
Kraiczy
Peter
P
Roversi
Pietro
P
Skerka
Christine
C
Kirschfink
Michael
M
Simon
Markus M
MM
Brade
Volker
V
Lowe
Edward D
ED
Zipfel
Peter
P
Wallich
Reinhard
R
Lea
Susan M
SM
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001665:Binding Sites; D025065:Borrelia burgdorferi / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012643:Selenium / Q000737:chemistry / Q000378:metabolism; D012645:Selenomethionine / Q000737:chemistry / Q000378:metabolism
D001426:Bacterial Proteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; C440651:complement regulator-acquiring surface proteins, Borrelia burgdorferi; D012645:Selenomethionine; D012643:Selenium