title: Antibacterial activity of silver inorganic agent YDA filler.
abstract: YDA filler is an antibacterial agent that is currently in commercial dental use. In this study, we attempted to determine whether it exerts an antibacterial effect on human saliva bacteria, and to determine whether it can be used in dental materials. CFUs in 1 mL stimulated human saliva were examined using blood agar and mitis salivarius agar after immersion, with or without YDA filler. The antibacterial effect was compared with that of Ketac-Silver. Dental materials containing 5% wt YDA filler were prepared for in vitro testing on S. mutans and A. viscosus. Furthermore, we examined the in vitro cytotoxicity of experimental MMA resin containing YDA filler on HeLa cells. Human saliva bacteria and mutans streptococci showed reduced viability following exposure to YDA filler after 12 h. The concentration of silver ions released by YDA filler was below 1 ppm after 12 h. Two tested strains showed reduced viability following exposure to dental materials containing YDA filler. In another experiment, MMA resin containing YDA filler did not show cytotoxicity on HeLa cells after 24- and 48-h exposure. Thus, YDA filler may help in the development of antibacterial dental materials, such as composite resin, glass-ionomer or temporary cement.
authors.affiliation: Department of General & Operative Dentistry, School of Dentistry, Asahi University, Japan. shizue@dent.asahi-u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Ohashi
authors.forename: S
authors.initials: S
authors.lastname: Saku
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D016924:Actinomyces viscosus / Q000187:drug effects; D000535:Aluminum / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D003188:Composite Resins / Q000627:therapeutic use; D003738:Dental Cements / Q000627:therapeutic use; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D010758:Phosphorus / Q000494:pharmacology*; D012463:Saliva / Q000382:microbiology*; D012834:Silver / Q000032:analysis; D013295:Streptococcus mutans / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D003188:Composite Resins; D003738:Dental Cements; D010758:Phosphorus; D012834:Silver; D000535:Aluminum