CD158k/KIR3DL2 is a new phenotypic marker of Sezary cells: relevance for the diagnosis and follow-up of Sezary syndrome.
CD158k molecules belong to the family of killer cell immunoglobulin-like receptors (KIR) that are expressed on a minor population of circulating NK and CD8+ T lymphocytes. Here, we report a strong positive correlation between the percentage of CD158k+ blood lymphocytes analyzed by flow cytometry and the percentage of atypical circulating cells (Sezary cells) determined by cytomorphology in a large group of patients with Sezary syndrome. Moreover, we show that circulating CD4+CD158k+ lymphocytes correspond to the malignant clonal cell population. Our findings suggest that the CD158k marker could be a useful tool for the evaluation of the circulating tumoral burden and the follow-up of patients with Sezary syndrome.
Poszepczynska-Guigné
Ewa
E
INSERM U448, Hôpital Henri Mondor, Créteil, France. ewa.guigne@libertysurf.fr
Schiavon
Valérie
V
D'Incan
Michel
M
Echchakir
Hamid
H
Musette
Philippe
P
Ortonne
Nicolas
N
Boumsell
Laurence
L
Moretta
Alessandro
A
Bensussan
Armand
A
Bagot
Martine
M
D014408:Biomarkers, Tumor / Q000097:blood*; D005434:Flow Cytometry; D005500:Follow-Up Studies; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry*; D010641:Phenotype; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D011971:Receptors, Immunologic / Q000097:blood*; D054340:Receptors, KIR; D054343:Receptors, KIR2DL2; D054347:Receptors, KIR3DL2; D012751:Sezary Syndrome / Q000175:diagnosis*; D012878:Skin Neoplasms / Q000175:diagnosis*
D014408:Biomarkers, Tumor; C515077:KIR2DL2 protein, human; C515084:KIR3DL2 protein, human; D016693:Receptors, Antigen, T-Cell, alpha-beta; D011971:Receptors, Immunologic; D054340:Receptors, KIR; D054343:Receptors, KIR2DL2; D054347:Receptors, KIR3DL2