title: Molecular epidemiology of outbreaks of viral gastroenteritis in New York State, 1998-1999.
abstract: This investigation evaluated the role of Norwalk-like virus (NLV) and other viruses (rotavirus, enteric adenovirus, and enterovirus) in 11 outbreaks of acute nonbacterial gastroenteritis that occurred in multiple settings in a span of 18 months in New York State. To determine the etiology of illness, patients' stool specimens were analyzed with a combination of reverse-transcription polymerase chain reaction (RT-PCR) and nucleotide sequencing, cell culture, and ELISA diagnostic techniques. NLV was detected from all of these outbreaks, with an overall detection rate of 64% (51 of 79) for all specimens tested. Repeated attempts to isolate other viral pathogens were unsuccessful. Phylogenetic analysis of a subset of 27 specimens from these outbreaks showed the presence of both genogroup I and genogroup II NLVs. A spectrum of different nucleotide sequences were detected, demonstrating interoutbreak sequence variation and unrelated infections. NLV is a significant causative agent of diarrhea outbreaks in New York State.
authors.affiliation: New York State Department of Health, Slingerlands, NY 12159, USA. nkc01@health.state.ny.us
authors.forename: Nando K
authors.initials: NK
authors.lastname: Chatterjee
authors.forename: Dennison W
authors.initials: DW
authors.lastname: Moore
authors.forename: Stephan S
authors.initials: SS
authors.lastname: Monroe
authors.forename: Roger I
authors.initials: RI
authors.lastname: Glass
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Cambridge
authors.forename: Stan F
authors.initials: SF
authors.lastname: Kondracki
authors.forename: Dale L
authors.initials: DL
authors.lastname: Morse
mesh_terms: D000595:Amino Acid Sequence; D017250:Caliciviridae Infections / Q000453:epidemiology* / Q000235:genetics; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay; D005759:Gastroenteritis / Q000453:epidemiology* / Q000235:genetics / Q000821:virology; D014644:Genetic Variation; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009518:New York / Q000453:epidemiology; D029322:Norovirus / Q000145:classification / Q000235:genetics; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D012367:RNA, Viral