[Laboratory research of the influence of keyouling on the proliferation of human prepuce epidermis cells and condyloma acuminatum cells].
OBJECTIVE
To discuss the mechanism of the Chinese medicine Keyouling in the treatment of condyloma axuminatum (CA).


METHODS
Human prepuce epidermis cells and CA cells were primarily cultured and subcultured. We determined the proliferation of human prepuce epidermis cells and CA cells, and observed the influence of Keyouling with different concentrations on the proliferation of human prepuce epidermis cells and CA cells by means of MTT colourimetry assay.


RESULTS
The absorbance was directly proportional to the numbers of human prepuce epidermis cells (r = 0.9850, P < 0.001) and CA cells (r = 0.9892, P < 0.001). Keyouling had no effect on proliferation of the human prepuce epidermis cells, but it had significant inhibition on CA cells. The concentrations of Keyouling bore negative correlation with the proliferation percentage of CA cells(r = -0.4124, P < 0.01).


CONCLUSIONS
Keyouling can significantly restrain the growth and proliferation of CA cells but has no damaging effect on normal organic cuticle cells. It is suggested that Keyouling might have anti-HPV effect.
Jiang
Yi
Y
Department of Andrology, General Hospital of Panzhihua Steel & Iron (Group) Co., Panzhihua, Sichuan 617023, China.
Zhang
Shuwu
S
Wang
Jiuyuan
J
Wan
Kaicheng
K
Wang
Caiming
C
D000293:Adolescent; D000328:Adult; D002455:Cell Division / Q000187:drug effects; D003218:Condylomata Acuminata / Q000188:drug therapy* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010409:Penile Diseases / Q000188:drug therapy* / Q000473:pathology; D010413:Penis / Q000187:drug effects; D012867:Skin / Q000187:drug effects
D004365:Drugs, Chinese Herbal