title: Lipid/peptide/nucleotide separation with MALDI-ion mobility-TOF MS.
abstract: Matrix-assisted laser desorption/ionization when combined with ion mobility-orthogonal time-of-flight mass spectrometry is a viable technique for fast separation and analysis of biomolecules in complex mixtures. Isobaric lipid, peptide, and oligonucleotide ions are preseparated before mass analysis by differences of up to 30% in mobility drift time. Ions of similar chemical type fall along well-defined "trend lines" (with deviations of approximately 3%) when plotted in two-dimensional representations of ion mobility as a function of m/z. Discussion of fundamental and technical limitations of the technique point to its potential for being most useful when applied to systems such as bodily fluids and intact tissue, where an alternative chemical or chromatographic preseparation step prior to mass analysis is either impractical or undesirable.
authors.affiliation: NIDA IRP, Baltimore, Maryland 21224, USA. awoods@intra.nida.nih.gov
authors.forename: Amina S
authors.initials: AS
authors.lastname: Woods
authors.forename: Michael
authors.initials: M
authors.lastname: Ugarov
authors.forename: Tom
authors.initials: T
authors.lastname: Egan
authors.forename: John
authors.initials: J
authors.lastname: Koomen
authors.forename: Kent J
authors.initials: KJ
authors.lastname: Gillig
authors.forename: Katrin
authors.initials: K
authors.lastname: Fuhrer
authors.forename: Marc
authors.initials: M
authors.lastname: Gonin
authors.forename: J Albert
authors.initials: JA
authors.lastname: Schultz
mesh_terms: D000701:Analgesics, Opioid / Q000032:analysis; D008055:Lipids / Q000737:chemistry*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D009020:Morphine / Q000032:analysis; D009711:Nucleotides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D012463:Saliva / Q000737:chemistry; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D000701:Analgesics, Opioid; D008055:Lipids; D009711:Nucleotides; D010455:Peptides; D009020:Morphine