title: Flow cytometric measurement of intracellular IFN-gamma induction in aged subjects before and after parenteral influenza vaccination.
abstract: Flow cytometric analysis, used to study intracellular expression of IFN-gamma in peripheral blood mononuclear cells (PBMC) from aged volunteers before and after parenteral influenza vaccination, was found capable of rapidly detecting influenza antigen induced variation of IFN-gamma expression. Although the vaccine was capable of generating a satisfactory antibody response, it did not stimulate an increase in the percentage of IFN-gamma positive cells.
authors.affiliation: Department of Hygiene and Public Health, University of Perugia, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Fringuelli
authors.forename: E
authors.initials: E
authors.lastname: Lepri
authors.forename: B
authors.initials: B
authors.lastname: Camilloni
authors.forename: M
authors.initials: M
authors.lastname: Neri
authors.forename: E
authors.initials: E
authors.lastname: Carriò
authors.forename: A
authors.initials: A
authors.lastname: Russano
authors.forename: F
authors.initials: F
authors.lastname: Spinozzi
authors.forename: A M
authors.initials: AM
authors.lastname: Iorio
mesh_terms: D000368:Aged; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D007371:Interferon-gamma / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D007252:Influenza Vaccines; D007371:Interferon-gamma