title: [A rapid procedure to purify serum IgG from Microtus fotis].
abstract: OBJECTIVE
  To evaluate the procedure to purify IgG antibodies from Microtus fotis serum.
  
  
  METHODS
  IgG antibodies from sera of three groups of Microtus fotis were purified by protein G or protein A affinity chromatography, their purity and binding capacity were compared.
  
  
  RESULTS
  The protein G affinity chromatography was more efficient than protein A affinity chromatography. The antibodies isolated from protein G affinity chromatography showed a higher purity and better activity than that from protein A affinity chromatography monitored by SDS-PAGE and ELISA. The ability of the purified IgG to bind the second antibodies were 8.5 times and 3.1 times that of non-IgG proteins and unpurified sera, respectively.
  
  
  CONCLUSIONS
  The protein G affinity chromatography is a rapid, convenient and reliable procedure for Microtus fotis serum IgG purification.
authors.affiliation: Shanghai Municipal Center for Disease Control & Prevention, Shanghai 200336.
authors.forename: Shou-fu
authors.initials: SF
authors.lastname: Jiang
authors.forename: Cai-e
authors.initials: CE
authors.lastname: Pan
authors.forename: Yan-yan
authors.initials: YY
authors.lastname: He
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Yao-jun
authors.initials: YJ
authors.lastname: Shi
authors.forename: Mei-xiong
authors.initials: MX
authors.lastname: Wei
mesh_terms: D000818:Animals; D003411:Arvicolinae; D002846:Chromatography, Affinity / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood / Q000302:isolation & purification*; D051379:Mice; D008815:Mice, Inbred Strains; D009419:Nerve Tissue Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley; D013205:Staphylococcal Protein A
chemical_list: C029152:G-substrate; D007074:Immunoglobulin G; D009419:Nerve Tissue Proteins; D013205:Staphylococcal Protein A