Adipose-derived adult stromal cells heal critical-size mouse calvarial defects.
In adults and children over two years of age, large cranial defects do not reossify successfully, posing a substantial biomedical burden. The osteogenic potential of bone marrow stromal (BMS) cells has been documented. This study investigates the in vivo osteogenic capability of adipose-derived adult stromal (ADAS) cells, BMS cells, calvarial-derived osteoblasts and dura mater cells to heal critical-size mouse calvarial defects. Implanted, apatite-coated, PLGA scaffolds seeded with ADAS or BMS cells produced significant intramembranous bone formation by 2 weeks and areas of complete bony bridging by 12 weeks as shown by X-ray analysis, histology and live micromolecular imaging. The contribution of implanted cells to new bone formation was 84-99% by chromosomal detection. These data show that ADAS cells heal critical-size skeletal defects without genetic manipulation or the addition of exogenous growth factors.
Cowan
Catherine M
CM
The Department of Surgery, Stanford University School of Medicine, Stanford University, 257 Campus Drive, Stanford, California 94305, USA.
Shi
Yun-Ying
YY
Aalami
Oliver O
OO
Chou
Yu-Fen
YF
Mari
Carina
C
Thomas
Romy
R
Quarto
Natalina
N
Contag
Christopher H
CH
Wu
Benjamin
B
Longaker
Michael T
MT
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D019344:Lactic Acid; D051379:Mice; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D012886:Skull / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology*
D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid