title: [Prevalence of AA(6')-APH(2")Ia gene among high-level aminoglycoside resistant Enterococci and Staphylococci].
abstract: According to new reports the AAC (6')-APH (2")Ia gene is no longer the only gene encoding resistance to gentamycin in Gram-positive cocci and therefore the current method for predicting synergism aminoglycosides with bacterial cell wall active agents in this bacteria may need revision. To further our knowledge of aminoglycoside resistance mechanism in Gram-positive cocci in Gdańsk region we tested presence of AAC (6')-APH (2")Ia gene among 22 enterococcal (E. faecalis) and 41 staphylococcal (S. haemolyticus, S. aureus, S. epidermidis) gentamycin-resistant isolates. Presence of AAC (6')-APH (2")Ia gene varied from 50% (n = 6) in gentamycin-resistant S. epidermidis, 80% (n = 10) in gentamycin resistant S. haemolyticus 88% in methicillin-resistant Staphylococcus aureus (MRSA) (n = 25). In Enterococcus faecalis this gene was noticed only in 59% (n = 22) of gentamycin-resistant isolates. These results suggest that spread of resistance gene among different species is limited and AAC (6')-APH (2")Ia mediated gentamycin-resistance mechanism is more common among MRSA and Staphylococcus haemolyticus.
authors.affiliation: Katedra i Zakład Mikrobiologii Lekarskiej AM w Gdańsku.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Jarzembowski
authors.forename: Ewa
authors.initials: E
authors.lastname: Michnowska-Swincow
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Wiśniewska
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D013293:Enterococcus faecalis / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D005839:Gentamicins / Q000494:pharmacology; D008712:Methicillin / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D015995:Prevalence; D013210:Staphylococcus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000235:genetics; D013212:Staphylococcus epidermidis / Q000235:genetics; D041162:Staphylococcus haemolyticus / Q000235:genetics
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D005839:Gentamicins; D008712:Methicillin