title: Reticulon 1-C/neuroendocrine-specific protein-C interacts with SNARE proteins.
abstract: Reticulons are proteins of neuroendocrine cells localized primarily to the endoplasmic reticulum membrane. Despite their implication in cellular processes like apoptosis or axonal regeneration, their intracellular molecular function is still largely unknown. Here, we show that reticulon 1-C can be detected in a protein complex of 150-200 kDa, and that a number of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins, i.e. syntaxin 1, syntaxin 7, syntaxin 13 and VAMP2, can be co-immunoprecipitated with reticulon 1-C. Moreover, it localizes to a nocodazole-sensitive, but calreticulin-negative domain of the endoplasmic reticulum. Finally, overexpression in PC12 cells of a reticulon 1-C fragment which binds to SNAREs, significantly enhances human growth hormone secretion. These results suggest that reticulons are involved in vesicle trafficking events, including regulated exocytosis.
authors.affiliation: Facultè des Sciences de la Vie, Ecole Polytechnique Fèdèrale de Lausanne, Lausanne, Switzerland.
authors.forename: Pascal
authors.initials: P
authors.lastname: Steiner
authors.forename: Karina
authors.initials: K
authors.lastname: Kulangara
authors.forename: J C Floyd
authors.initials: JC
authors.lastname: Sarria
authors.forename: Liliane
authors.initials: L
authors.lastname: Glauser
authors.forename: Romano
authors.initials: R
authors.lastname: Regazzi
authors.forename: Harald
authors.initials: H
authors.lastname: Hirling
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D002478:Cells, Cultured; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism; D005089:Exocytosis / Q000502:physiology; D019382:Human Growth Hormone; D006801:Humans; D007106:Immune Sera / Q000378:metabolism; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D016716:PC12 Cells; D011233:Precipitin Tests; D011485:Protein Binding / Q000502:physiology; D021381:Protein Transport / Q000502:physiology; D050765:Qa-SNARE Proteins; D050683:R-SNARE Proteins; D051381:Rats; D050600:SNARE Proteins; D050827:Syntaxin 1; D033921:Vesicular Transport Proteins
chemical_list: D000954:Antigens, Surface; D007106:Immune Sera; D046911:Macromolecular Substances; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D050765:Qa-SNARE Proteins; D050683:R-SNARE Proteins; C081781:RTN1 protein, human; C489913:Rtn1 protein, rat; D050600:SNARE Proteins; C494212:STX1A protein, human; C494213:Stx1a protein, rat; D050827:Syntaxin 1; D033921:Vesicular Transport Proteins; D019382:Human Growth Hormone