title: Laboratory practices for stool-specimen culture for bacterial pathogens, including Escherichia coli O157:H7, in the FoodNet sites, 1995-2000.
abstract: In 2000, we surveyed microbiologists in 388 clinical laboratories, which tested an estimated 339,000 stool specimens in 1999, about laboratory methods and policies for the routine testing of stool specimens for Salmonella, Shigella, Campylobacter, and Vibrio species, Yersinia entercolitica, and Escherichia coli O157:H7. The results were compared with those of similar surveys conducted in 1995 and 1997. Although these laboratories reported routinely testing for Salmonella, Shigella, and Campylobacter species, only 57% routinely tested for E. coli O157:H7, 50% for Y. entercolitica, and 50% for Vibrio species. The mean proportions of stool specimens that yielded these pathogens were as follows: Campylobacter, 1.3% of specimens; Salmonella, 0.9%; Shigella, 0.4%; and E. coli O157:H7, 0.3%. The proportion of laboratories that routinely tested for E. coli O157:H7 increased from 59% in 1995 to 68% in 2000; however, the proportion of stool specimens tested decreased from 53% to 46%. E. coli O157:H7 should be routinely sought in stool specimens submitted for microbiologic culture.
authors.affiliation: Foodborne and Diarrheal Diseases Branch, Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Voetsch
authors.forename: Frederick J
authors.initials: FJ
authors.lastname: Angulo
authors.forename: Terry
authors.initials: T
authors.lastname: Rabatsky-Ehr
authors.forename: Sue
authors.initials: S
authors.lastname: Shallow
authors.forename: Maureen
authors.initials: M
authors.lastname: Cassidy
authors.forename: Stephanie M
authors.initials: SM
authors.lastname: Thomas
authors.forename: Ellen
authors.initials: E
authors.lastname: Swanson
authors.forename: Shelley M
authors.initials: SM
authors.lastname: Zansky
authors.forename: Marguerite A
authors.initials: MA
authors.lastname: Hawkins
authors.forename: Timothy F
authors.initials: TF
authors.lastname: Jones
authors.forename: Pamela J
authors.initials: PJ
authors.lastname: Shillam
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Van Gilder
authors.forename: Joy G
authors.initials: JG
authors.lastname: Wells
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Griffin
mesh_terms: D002167:Campylobacter; D019411:Clinical Laboratory Techniques; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology; D019453:Escherichia coli O157; D005243:Feces / Q000382:microbiology*; D006801:Humans; D012475:Salmonella; D012760:Shigella; D014481:United States / Q000453:epidemiology; D014733:Vibrio; D015007:Yersinia