title: A novel kinesin-like protein, KIF1Bbeta3 is involved in the movement of lysosomes to the cell periphery in non-neuronal cells.
abstract: The kinesin superfamily protein, KIF1Bbeta, a splice variant of KIF1B, is involved in the transport of synaptic vesicles in neuronal cells, and is also expressed in various non-neuronal tissues. To elucidate the functions of KIF1Bbeta in non-neuronal cells, we analyzed the intracellular localization of KIF1Bbeta and characterized its isoform expression profile. In COS-7 cells, KIF1B colocalized with lysosomal markers and expression of a mutant form of KIF1Bbeta, lacking the motor domain, impaired the intracellular distribution of lysosomes. A novel isoform of the kinesin-like protein, KIF1Bbeta3, was identified in rat and simian kidney. It lacks the 5th exon of the KIF1Bbeta-specific tail region. Overexpression of KIF1Bbeta3 induced the translocation of lysosomes to the cell periphery. However, overexpression of KIF1Bbeta3-Q98L, which harbors a pathogenic mutation associated with a familial neuropathy, Charcot-Marie-Tooth disease type 2 A, resulted in the abnormal perinuclear clustering of lysosomes. These results indicate that KIF1Bbeta3 is involved in the translocation of lysosomes from perinuclear regions to the cell periphery.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, Osaka University, Machikaneyama-cho 1-16, Toyonaka City, Osaka, Japan 560-0043.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Matsushita
authors.forename: Shingo
authors.initials: S
authors.lastname: Tanaka
authors.forename: Norihiro
authors.initials: N
authors.lastname: Nakamura
authors.forename: Hiroki
authors.initials: H
authors.lastname: Inoue
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kanazawa
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001483:Base Sequence; D019556:COS Cells; D002467:Cell Nucleus / Q000378:metabolism; D002607:Charcot-Marie-Tooth Disease / Q000235:genetics / Q000378:metabolism; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism; D011992:Endosomes / Q000737:chemistry / Q000378:metabolism; D005973:Glutamine / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism*; D016547:Kinesins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051907:Lysosomal Membrane Proteins; D008247:Lysosomes / Q000378:metabolism*; D020409:Molecular Motor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D051381:Rats; D016415:Sequence Alignment; D013347:Subcellular Fractions
chemical_list: D015703:Antigens, CD; C490399:Kif1b protein, rat; D051907:Lysosomal Membrane Proteins; D020409:Molecular Motor Proteins; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D005973:Glutamine; D016547:Kinesins