Total proteoglycan and chondroitin-4-sulfate levels in gingiva of patients with various types of periodontitis.
BACKGROUND
The aim of the present study was to investigate the total proteoglycan (PG) and chondroitin-4-sulfate (C4S) levels in gingival tissue samples obtained from patients with aggressive periodontitis (AgP) and chronic periodontitis (CP) before therapy (baseline) and 1 month after completion of non-surgical periodontal therapy.


METHODS
Gingival tissue samples were obtained from 10 AgP and 10 CP patients before initiation of treatment (baseline) and 1 month after non-surgical periodontal treatment. The control group comprised 10 systemically and periodontally healthy subjects. Total PG and C4S levels were determined by biochemical techniques. PG levels were analyzed using a modified Bitter and Muir method. C4S assay was carried out using chondroitin sulphate lyase AC and chondroitin-6 sulphate sulphohydrolase enzymes. The results were tested statistically using parametric tests.


RESULTS
The clinical periodontal parameters demonstrated significant decreases in the periodontitis groups (P<0.05) after treatment, and there was no significant difference between AgP and CP groups at baseline and after treatment (P>0.05). At baseline, total PG and C4S levels in both of the periodontitis groups were significantly lower than that of the control group (P<0.05). One month after the non-surgical periodontal treatment, total PG levels in the periodontitis groups were comparable to the control group (P>0.05), whereas C4S levels in the AgP group were significantly lower than the other study groups (P<0.05). In the CP group, total PG and C4S levels increased significantly (P = 0.001 and P = 0.006, respectively) after non-surgical periodontal treatment, but they did not increase in the AgP group (P>0.05).


CONCLUSIONS
The significant increases observed in total proteoglycan and chondroitin-4-sulfate levels after non-surgical periodontal treatment in the CP group but not in the AgP group may suggest that healing patterns differ between the two periodontitis types in terms of PG and C4S composition of extracellular matrix.
Vardar
Saynur
S
Department of Periodontology, School of Dentistry, Ege University, Izmir, Turkey. saynurvardar@yahoo.com
Baylas
Haluk
H
Zihnioğlu
Figen
F
Emingil
Gülnur
G
Buduneli
Nurcan
N
Atilla
Gül
G
D000328:Adult; D000704:Analysis of Variance; D016009:Chi-Square Distribution; D002809:Chondroitin Sulfates / Q000032:analysis*; D002908:Chronic Disease; D005260:Female; D005500:Follow-Up Studies; D005881:Gingiva / Q000737:chemistry*; D006025:Glycosaminoglycans / Q000032:analysis; D006801:Humans; D008297:Male; D010512:Periodontal Index; D010518:Periodontitis / Q000145:classification / Q000378:metabolism* / Q000628:therapy; D011509:Proteoglycans / Q000032:analysis*; D014945:Wound Healing
D006025:Glycosaminoglycans; D011509:Proteoglycans; D002809:Chondroitin Sulfates