Application of a yeast method for DNA extraction associated with database interrogations for the characterization of various filamentous fungi from diseased hop.
Filamentous fungi were collected from diseased hop (Humulus lupulus; L.) and DNA was prepared from 19 isolates. The critical step of cell lysis was carried out using zymolyase for fungal cell wall digestion. DNA was successfully prepared for all isolates and allowed ribosomal DNA region amplifications by PCR. The amplicons were sequenced and sequences were compared with nucleotides databases. These analyses allowed identification of each fungus and gave a precise view of the ecosystem. Seven different genera were found among the 19 isolates. A phylogenetic tree was constructed and revealed the diversity of the hop plant environment.
Phalip
Vincent
V
UMR 7100, Université Louis Pasteur-CNRS, Ecole Supérieure de Biotechnologie de Strasbourg, Boulevard Sébastien Brant, BP 10413, 67412 Illkirch Cedex, France. vincent.phalip@esbs.u-strasbg.fr
Hatsch
Didier
D
Jeltsch
Jean-Marc
JM
D001483:Base Sequence; D004271:DNA, Fungal / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D020869:Gene Expression Profiling / Q000379:methods*; D027582:Humulus / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods*; D012689:Sequence Homology, Nucleic Acid; D013048:Specimen Handling / Q000379:methods*; D015003:Yeasts / Q000235:genetics*
D004271:DNA, Fungal