title: Comparative genomic hybridization analysis of a pleuropulmonary blastoma.
abstract: Pleuropulmonary blastoma (PPB) is a rare, aggressive dysontogenetic tumor of childhood. We report the comparative genomic hybridization (CGH) study performed on a case of PPB in a 3-year-old-boy. The tumor was characterized by several chromosomal imbalances. Gains observed affected regions: 1q12-q23, 3q23-qter, 8pter-q24.1, 9p13-q21, 17p12-p11, 17q11-q22, 17q23-q25, 19pter-p11, and 19q11-q13.3. Whole chromosome gains were detected at 2 and 7. Loss of genetic material was found at regions: 6q13-qter, 10pter-p13, 10q22-qter, and 20p13. To our knowledge, there have been no CGH reports on PPB, but it is interesting to note that 1) the alterations found confirm previous cytogenetic reports describing gains of chromosomes 2 and 8 as recurrent abnormalities in this type of tumor, suggesting that a gene or genes of putative relevance in PPB pathogenesis are mapped at 8p11-p12, and 2) the CGH profile of this case is very similar to those observed in embryonal rhabdomyosarcomas, in which gains of 2 or 2q, 7 or 7q, and 8 or 8p and loss of 10q22-qter are consistently found. This finding supports the hypothesis that PPB may be tumorigenetically related with embryonal rhabdomyosarcoma.
authors.affiliation: Cytometry and Cytogenetic Laboratory, Center for Immunology and Molecular Pathology, Portuguese Cancer Institute, R. Prof. Lima Basto 1099-023, Lisbon, Portugal. lroque@ipolisboa.min-saude.pt
authors.forename: Lúcia
authors.initials: L
authors.lastname: Roque
authors.forename: Raquel
authors.initials: R
authors.lastname: Rodrigues
authors.forename: Carmo
authors.initials: C
authors.lastname: Martins
authors.forename: Catarina
authors.initials: C
authors.lastname: Ribeiro
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Ribeiro
authors.forename: António Gentil
authors.initials: AG
authors.lastname: Martins
authors.forename: Pedro
authors.initials: P
authors.lastname: Oliveira
authors.forename: Isabel
authors.initials: I
authors.lastname: Fonseca
mesh_terms: D002675:Child, Preschool; D002869:Chromosome Aberrations; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D006801:Humans; D007124:Immunoenzyme Techniques; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008175:Lung Neoplasms / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D009693:Nucleic Acid Hybridization; D010997:Pleural Neoplasms / Q000175:diagnosis / Q000235:genetics*; D018202:Pulmonary Blastoma / Q000175:diagnosis / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm