16S to 23S rDNA spacer fragment length polymorphism of Aeromonas sp.
We analyzed polymorphism of the PCR-amplified 16S-23S rDNA spacer of Aeromonas species. A total of 69 isolates representing 18 DNA hybridization groups were used in this study. The analysis of PCR products of 16S-23S rDNA spacers revealed patterns consisting of two to eight DNA fragments. The fragment sizes ranged from 730 to 1050 bp. DNA patterns revealed a considerable genetic diversity between species and within a species. When a procedure to eliminate heteroduplex formation was performed, the number of bands was reduced to 2-5. Nevertheless the homoduplex ISR (intergenic spacer region) patterns obtained were not useful for species distinguishing.
Laganowska
Marzena
M
Department of Microbiology, Institute of Experimental Biology, Adam Mickiewicz University, Fredry 10, 61-701 Poznań, Poland.
Kaznowski
Adam
A
D000333:Aeromonas / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014871:Water Microbiology
D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer