title: Translational accuracy during exponential, postdiauxic, and stationary growth phases in Saccharomyces cerevisiae.
abstract: When the yeast Saccharomyces cerevisiae shifts from rapid growth on glucose to slow growth on ethanol, it undergoes profound changes in cellular metabolism, including the destruction of most of the translational machinery. We have examined the effect of this metabolic change, termed the diauxic shift, on the frequency of translational errors. Recoding sites are mRNA sequences that increase the frequency of translational errors, providing a convenient reporter of translational accuracy. We found that the diauxic shift causes no overall change in translational accuracy but does cause a strong reduction in the frequency of one type of programmed error: Ty +1 frameshifting. Genetic data suggest that this effect may be due to changes in the relative amounts of tRNA participating in translation elongation. We discuss possible implications for expression strategies that use recoding.
authors.affiliation: Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, Maryland 21250, USA. stahl@infobiogen.fr
authors.forename: Guillaume
authors.initials: G
authors.lastname: Stahl
authors.forename: Samia N Ben
authors.initials: SN
authors.lastname: Salem
authors.forename: Lifeng
authors.initials: L
authors.lastname: Chen
authors.forename: Bing
authors.initials: B
authors.lastname: Zhao
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Farabaugh
mesh_terms: D003062:Codon / Q000502:physiology; D000431:Ethanol / Q000378:metabolism; D018965:Frameshifting, Ribosomal / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D014176:Protein Biosynthesis; D012346:RNA, Transfer, Amino Acyl / Q000502:physiology; D018626:Retroelements / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000502:physiology
chemical_list: D003062:Codon; D012346:RNA, Transfer, Amino Acyl; D018626:Retroelements; D000431:Ethanol; D005947:Glucose