title: [HER2 gene amplification assay: is CISH an alternative to FISH?].
abstract: The HER2 proto-oncogene encodes a transmembrane protein, which is considered to function as a growth factor receptor. Overexpression of this protein found by immunohistochemistry in about 20% of infiltrating breast carcinomas, has a predictive value of response to treatment by trastuzumab, an anti-HER2 humanized monoclonal antibody. Search for HER2 gene amplification is necessary to adapt the immunohistochemical technique quality and also in the cases of delicate analysis or weak overexpression. It is usually carried out by Fluorescence In Situ Hybridization (FISH). A more recent hybridization technique, named CISH because of its chromogenic revelation is an alternative method, which gives highly correlated results with FISH. We present details of this technique, which may be more familiar for the pathologists than FISH, because reading analysis is similar to that of immunohistochemical staining.
authors.affiliation: Service d'Anatomie Pathologique, Centre François Baclesse, 14076 Caen cedex 5. y.denoux@baclesse.fr
authors.forename: Yves
authors.initials: Y
authors.lastname: Denoux
authors.forename: Laurent
authors.initials: L
authors.lastname: Arnould
authors.forename: Maryse
authors.initials: M
authors.lastname: Fiche
authors.forename: B
authors.initials: B
authors.lastname: Lannes
authors.forename: Jérôme
authors.initials: J
authors.lastname: Couturier
authors.forename: Anne
authors.initials: A
authors.lastname: Vincent-Salomon
authors.forename: Frédérique
authors.initials: F
authors.lastname: Penault-Llorca
authors.forename: M
authors.initials: M
authors.lastname: Antoine
authors.forename: A
authors.initials: A
authors.lastname: Balaton
authors.forename: M C
authors.initials: MC
authors.lastname: Baranzelli
authors.forename: V
authors.initials: V
authors.lastname: Becette
authors.forename: J P
authors.initials: JP
authors.lastname: Bellocq
authors.forename: F
authors.initials: F
authors.lastname: Bibeau
authors.forename: F
authors.initials: F
authors.lastname: Ettore
authors.forename: V
authors.initials: V
authors.lastname: Fridman
authors.forename: J P
authors.initials: JP
authors.lastname: Gnassia
authors.forename: J
authors.initials: J
authors.lastname: Jacquemier
authors.forename: G
authors.initials: G
authors.lastname: MacGrogan
authors.forename: M C
authors.initials: MC
authors.lastname: Mathieu
authors.forename: C
authors.initials: C
authors.lastname: Migeon
authors.forename: C
authors.initials: C
authors.lastname: Rigaud
authors.forename: P
authors.initials: P
authors.lastname: Roger
authors.forename: B
authors.initials: B
authors.lastname: Sigal-Zafrani
authors.forename: J
authors.initials: J
authors.lastname: Simony-Lafontaine
authors.forename: M
authors.initials: M
authors.lastname: Trassard
authors.forename: I
authors.initials: I
authors.lastname: Treilleux
authors.forename: V
authors.initials: V
authors.lastname: Verriele
authors.forename: J J
authors.initials: JJ
authors.lastname: Voigt
mesh_terms: D001943:Breast Neoplasms / Q000737:chemistry / Q000235:genetics; D018270:Carcinoma, Ductal, Breast / Q000737:chemistry / Q000235:genetics; D002863:Chromogenic Compounds / Q000032:analysis*; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics; D015342:DNA Probes; D004076:Digoxigenin / Q000032:analysis; D005260:Female; D018734:Genes, erbB-2; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence; D021141:Nucleic Acid Amplification Techniques; D000090063:Proto-Oncogene Mas; D013048:Specimen Handling
chemical_list: D002863:Chromogenic Compounds; D015342:DNA Probes; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D004076:Digoxigenin