title: Supporting cells as a target of cisplatin-induced inner ear damage: therapeutic implications.
abstract: OBJECTIVE
  Cisplatin induces adverse effects such as ototoxicity and nephrotoxicity. Unexplained hearing improvement in sudden deafness and fluctuating hearing loss suggests the presence of some mechanisms other than the primary lesion of the outer hair cell. The authors' aim was to study the functional and morphological lesions induced in the cochlea of the rat after cisplatin injection.
  
  
  METHODS
  Prospective experimental morphological and functional study.
  
  
  METHODS
  Forty-eight Wistar rats were given intraperitoneal injections of 5 mg/kg cisplatin. After short-, medium-, and long-term survival, animals were studied by means of auditory brainstem response testing and scanning electronic microscopy.
  
  
  RESULTS
  An increased hearing loss was observed in animals with long-term survival compared with animals with short-term survival after a single dose of cisplatin. These findings correlated with a more intense injury of the supporting cells, continuing with the late deterioration of the hair cells.
  
  
  CONCLUSIONS
  The study suggested that the initial damage of the supporting cells could be responsible for the late injury of the hair cells. This hypothesis was supported by the late increase in hearing loss after a single dose of cisplatin, emphasizing the role of the supporting cells in the cochlear changes induced by cisplatin.
authors.affiliation: Department of Otolaryngology, Clínica Puerta de Hierro, Autónoma University, Madrid, Spain. rramirezc@seorl.net
authors.forename: Rafael
authors.initials: R
authors.lastname: Ramírez-Camacho
authors.forename: José Ramón
authors.initials: JR
authors.lastname: García-Berrocal
authors.forename: Julia
authors.initials: J
authors.lastname: Buján
authors.forename: Alejandro
authors.initials: A
authors.lastname: Martín-Marero
authors.forename: Almudena
authors.initials: A
authors.lastname: Trinidad
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D002945:Cisplatin / Q000633:toxicity*; D006198:Hair Cells, Auditory / Q000187:drug effects* / Q000648:ultrastructure; D034381:Hearing Loss / Q000139:chemically induced*; D008855:Microscopy, Electron, Scanning; D011446:Prospective Studies; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000970:Antineoplastic Agents; D002945:Cisplatin