Determination of dissociation constants by competitive binding in partial filling capillary electrophoresis.
The determination of dissociation constands (K(d)) by competitive ligand binding in partial filling capillary electrophoresis is demonstrated. Two different strategies were applied, one of which only uses a single reporter ligand and a more elaborated one which suppresses systemic disturbances by using a racemic mixture as reporter. The dissociation constants obtained by both alternatives were virtually identical and in good agreement with those previously reported.
Nilsson
Mikael
M
Department of Chemistry and Biomedical Sciences, University of Kalmar, Kalmar, Sweden.
Johansson
Gunnar
G
Isaksson
Roland
R
D000526:Alprenolol / Q000378:metabolism; D001667:Binding, Competitive; D019075:Electrophoresis, Capillary / Q000379:methods*; D008689:Methacrylates / Q000378:metabolism; D011433:Propranolol / Q000378:metabolism; D012821:Silanes / Q000378:metabolism; D013237:Stereoisomerism
D008689:Methacrylates; D012821:Silanes; C017492:methacryloxypropyltrimethoxysilane; D000526:Alprenolol; D011433:Propranolol