title: Sequence analysis and genomic organization of a new insect picorna-like virus, Ectropis obliqua picorna-like virus, isolated from Ectropis obliqua.
abstract: The complete nucleotide sequence of a new insect picorna-like virus, Ectropis obliqua picorna-like virus (EoPV), which causes a fatal infection of Ectropis obliqua larvae, has been determined. The genomic RNA of EoPV is 9394 nt in length and contains a single, large open reading frame (nt 391-9351) encoding a polyprotein of 2987 aa. Sequence comparisons with other viral polyproteins revealed that the consensus sequences for picornavirus RNA helicase, protease and RNA-dependent RNA polymerase proteins are found on the genome in order in the 5'-->3' direction. All structural genes were located at the 5' terminus. In terms of sequence similarity, identity and genome organization, EoPV resembles mammalian picornaviruses and three other insect picorna-like viruses: Infectious flacherie virus of silkworm, Sacbrood virus of honeybee and Perina nuda picorna-like virus (PnPV). Phylogenetic analysis showed that EoPV is most closely related to PnPV and suggests that these four insect picorna-like viruses might constitute a new group of insect-infectious RNA viruses.
authors.affiliation: Laboratory of Insect Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province 430072, China.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Wang
authors.affiliation: Laboratory of Insect Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province 430072, China.
authors.forename: Jiamin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Laboratory of Insect Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province 430072, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Lu
authors.affiliation: Laboratory of Insect Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province 430072, China.
authors.forename: Fuming
authors.initials: F
authors.lastname: Yi
authors.affiliation: Laboratory of Insect Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province 430072, China.
authors.forename: Chuanfeng
authors.initials: C
authors.lastname: Liu
authors.affiliation: Laboratory of Insect Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province 430072, China.
authors.forename: Yuanyang
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D036022:Capsid Proteins / Q000235:genetics; D010450:Endopeptidases / Q000235:genetics; D016679:Genome, Viral; D007313:Insecta / Q000502:physiology / Q000821:virology*; D007814:Larva / Q000821:virology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010849:Picornaviridae / Q000737:chemistry / Q000235:genetics*; D020365:RNA Helicases / Q000235:genetics; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D016415:Sequence Alignment; D017421:Sequence Analysis; D013662:Tea / Q000469:parasitology; D017361:Viral Nonstructural Proteins / Q000235:genetics
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral; D013662:Tea; D017361:Viral Nonstructural Proteins; D012324:RNA-Dependent RNA Polymerase; D010450:Endopeptidases; D020365:RNA Helicases