Characterization and purification of acidocin CH5, a bacteriocin produced by Lactobacillus acidophilus CH5.
OBJECTIVE
To characterize and to purify a bacteriocin produced by Lactobacillus acidophilus strain with its activity restricted to Gram-positive bacteria.


RESULTS
Native acidocin CH5, a bacteriocin produced by L. acidophilus CH5 an isolate from a dairy starter culture forms in MRS (Oxoid, Basingstoke, UK) broth high-molecular weight aggregates which can dissociate into smaller units (retained by 5 kDa membrane) with higher activity. Acidocin CH5 was purified using combinations of chromatographic methods based on hydrophobic and cation exchange principles and the N-terminal region was sequenced.


CONCLUSIONS
Based on our results it is evident that acidocin CH5 belongs, according to bacteriocin classification, to the class II bacteriocins with identical N-terminal amino acid sequence described in the literature previously.


CONCLUSIONS
The study has provided further data on bacteriocin acidocin CH5 from class II with wide spectrum of antimicrobial activity atypical for bacteriocins produced by L. acidophilus sharing the same homology.
Chumchalová
J
J
Department of Dairy and Fat Technology, Institute of Chemical Technology, Prague, Czech Republic. jana.chumchalova@vscht.cz
Stiles
J
J
Josephsen
J
J
Plocková
M
M
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000302:isolation & purification*; D001430:Bacteriocins / Q000096:biosynthesis / Q000302:isolation & purification*; D003470:Culture Media; D003902:Detergents / Q000494:pharmacology; D007779:Lactobacillus acidophilus / Q000187:drug effects / Q000378:metabolism*; D012997:Solvents; D014462:Ultrafiltration / Q000379:methods
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D003470:Culture Media; D003902:Detergents; D012997:Solvents; C488121:acidocin CH5, Lactobacillus acidophilus