title: [Analysis of antibacterial-relative proteins and peptides in housefly larvae].
abstract: OBJECTIVE
  To study the antibacterial-relative proteins and peptides in housefly larvae.
  
  
  METHODS
  Third-instar larvae of housefly were treated with different methods including pricking, pricking with bacteria, heating and ultrasonication and were extracted respectively after 6 h, 16 h, 24 h, 48 h, 65 h. After liquid phase assay, the antibacterial-relative proteins and peptides were confirmed with stepwise regression analysis.
  
  
  RESULTS
  The four treatments could all induce antibacterial substances. Among these induced proteins, X17 (20 kDa), X16 (22 kDa) had activity against Staphylococcus aureus and Bacillus subtilis, but X22 (13 kDa), X20 (16 kDa), X12 (26 kDa) had activity only against either Staphylococcus aureus or Bacillus subtilis. X7 (37 kDa), X5 (44 kDa) had negative contribution to antibacterial activity.
  
  
  CONCLUSIONS
  Many antibacterial-relative proteins and peptides which cooperate and establish a whole resisting system will begin to be translated or be translated more.
authors.affiliation: College of Life Science, Nankai University, Tianjin 300071, China.
authors.forename: Chunju
authors.initials: C
authors.lastname: An
authors.forename: Desen
authors.initials: D
authors.lastname: Li
authors.forename: Rongqian
authors.initials: R
authors.lastname: Du
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects; D006793:Houseflies / Q000737:chemistry*; D019476:Insect Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D007814:Larva / Q000737:chemistry; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D012044:Regression Analysis; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D019476:Insect Proteins; D010455:Peptides