title: High-fat diet induced adiposity in mice with targeted disruption of the dopamine-3 receptor gene.
abstract: Dopamine (DA) signaling has been implicated in the control of energy balance and ingestive behavior. In the present study, we sought to characterize body weight, body fat and food intake regulation in a mouse with a targeted disruption of the dopamine-3 receptor gene (Drd3). In the first set of experiments male and female wild-type and mutant (Drd3-/-) mice were given access to two different diets varying in fat content. Body weight, food intake, carcass analysis and plasma levels of leptin and insulin were measured. Male Drd3-/- mice have increased body weight and body fat when given access to high fat (HF) diet but not standard rodent chow. The female Drd3-/- mice did not demonstrate increased body weight when given access to either diet, but did have increased body fat on both diets. Plasma leptin and insulin levels reflected the increased adiposity demonstrated in each genotype and gender. These findings suggest the D3-R signaling is involved in the regulation of body weight and body fat when mice are given access to diets differing in palatability and fat content.
authors.affiliation: Department of Psychiatry, University of Cincinnati, P.O. Box 670559, Cincinnati, OH 45267-0559, USA.
authors.forename: John-Andrews
authors.initials: JA
authors.lastname: McQuade
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Benoit
authors.forename: Ming
authors.initials: M
authors.lastname: Xu
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Woods
authors.forename: Randy J
authors.initials: RJ
authors.lastname: Seeley
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects* / Q000502:physiology; D000704:Analysis of Variance; D000818:Animals; D001823:Body Composition / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D004041:Dietary Fats / Q000494:pharmacology*; D004435:Eating / Q000187:drug effects; D002149:Energy Intake / Q000187:drug effects; D005260:Female; D020868:Gene Silencing / Q000187:drug effects*; D007328:Insulin / Q000378:metabolism; D020738:Leptin / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout / Q000235:genetics; D017448:Receptors, Dopamine D2 / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D050637:Receptors, Dopamine D3; D012737:Sex Factors; D013997:Time Factors
chemical_list: D004041:Dietary Fats; C493594:Drd3 protein, mouse; D007328:Insulin; D020738:Leptin; D017448:Receptors, Dopamine D2; D050637:Receptors, Dopamine D3