Non-relation of parathyroid hormone gene polymorphisms to secondary hyperparathyroidism in Chinese hemodialysis patients.
OBJECTIVE
To investigate the relationship between parathyroid hormone (PTH) gene polymorphisms and secondary hyperparathyroidism in Chinese hemodialysis (HD) patients.


METHODS
210 patients in one HD unit were enrolled as the study group and 203 age-matched health subjects were enrolled as controls. PTH genotypes were determined by polymerase chain reaction and restricted fragment length polymorphisms (RFLPs) of BstBI and DraII. B and b or D and d were indicated by the presence or absence, respectively, of BstBI and DraII at restriction sites on the PTH gene. Serum iPTH levels and serum total calcium (Sca), phosphate (Sp), bone-specific alkaline phosphatase (bAlk), and aluminum (Al) levels across different genotypes were compared.


RESULTS
When polymorphisms at the BstBI the restriction site were investigated, the patients' BB, Bb, and bb genotype frequencies were 62%, 37%, and 1%, respectively. At the DraII restriction site, the patients' DD, Dd, and dd genotype frequencies were 78%, 17%, and 5%, respectively. No significant differences were found across the three genotypes, BB, Bb, and bb, in serum Sca, Sp, bAlk, Al and iPTH levels. DraII cleavage PTH gene polymorphisms displayed a similar pattern. Patients with BBDD haplotype were not found to have significantly higher serum iPTH levels than those with other haplotypes.


CONCLUSIONS
Our study appears to indicate that PTH gene polymorphisms do not influence serum iPTH levels in Chinese HD patients.
Chen
Jin-Bor
JB
Division of Nephrology, Department of Internal Medicine, Chang Gung Memorial Hospital, Kaohsiung, Taiwan, Republic of China. jbchen1@ms5.hinet.net
Chou
Fong-Fu
FF
Hsu
Kuo-Tai
KT
D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005838:Genotype; D006801:Humans; D006962:Hyperparathyroidism, Secondary / Q000235:genetics*; D007676:Kidney Failure, Chronic / Q000097:blood / Q000628:therapy; D008297:Male; D008875:Middle Aged; D010281:Parathyroid Hormone / Q000097:blood* / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D006435:Renal Dialysis
D010281:Parathyroid Hormone