title: Role of the midbody matrix in cytokinesis: RNAi and genetic rescue analysis of the mammalian motor protein CHO1.
abstract: CHO1 is a kinesin-like motor protein essential for cytokinesis in mammalian cells. To analyze how CHO1 functions, we established RNAi and genetic rescue assays. CHO1-depleted cells reached a late stage of cytokinesis but fused back to form binucleate cells because of the absence of the midbody matrix in the middle of the intercellular bridge. Expression of exogenous CHO1 restored the formation of the midbody matrix and rescued cytokinesis in siRNA-treated cells. By analyzing phenotypes rescued with different constructs, it was shown that both motor and stalk domains function in midbody formation, whereas the tail is essential for completion of cytokinesis after the midbody matrix has formed. During the terminal stage of cytokinesis, different subregions of the tail play distinctive roles in stabilizing the midbody matrix and maintaining an association between the midbody and cell cortex. These results demonstrate that CHO1 consists of functionally differentiated subregions that act in concert to ensure complete cell separation.
authors.affiliation: Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota 55455, USA.
authors.forename: Jurgita
authors.initials: J
authors.lastname: Matuliene
authors.forename: Ryoko
authors.initials: R
authors.lastname: Kuriyama
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D016466:CHO Cells; D002467:Cell Nucleus / Q000737:chemistry / Q000276:immunology; D006224:Cricetinae; D003412:Cricetulus; D048749:Cytokinesis / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D016547:Kinesins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D019913:Nuclear Localization Signals / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000494:pharmacology
chemical_list: D019913:Nuclear Localization Signals; D034741:RNA, Small Interfering; D000255:Adenosine Triphosphate; D016547:Kinesins