An in vitro study of different extracts and fractions of Allium sativum (garlic): vascular reactivity.
The aim of this study was to investigate the effect of different novel extracts and fractions obtained from Allium sativum (garlic) on in vitro vessel contraction in order to deepen our knowledge of their mechanism of action on vascular reactivity. The contraction induced by noradrenaline (NE, 10(-6) or 10(-5) M) or KCl (80 mM) was relaxed with all the extracts and fractions studied, but this effect was higher with RG 20-100 (raw garlic fraction) and FG 20-100 (frozen garlic fraction). To increase our understanding of the mechanisms of action of RG 200-100 and FG 200-100, we found their inhibitory actions were retained in the absence of endothelium, whereas inhibition of the entry of extracellular calcium and mobilization of intracellular calcium may play an instrumental role.
Ganado
Patricia
P
Department of Pharmacology, School of Medicine, Universidad Complutense de Madrid, Madrid, Spain.
Sanz
Mercedes
M
Padilla
Eugenia
E
Tejerina
Teresa
T
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects* / Q000502:physiology; D005615:Freezing; D005737:Garlic / Q000737:chemistry*; D008297:Male; D008638:Mesenteric Arteries / Q000187:drug effects* / Q000502:physiology; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D009638:Norepinephrine / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D010936:Plant Extracts; D011189:Potassium Chloride; D009638:Norepinephrine