title: A glassy carbon microfluidic device for electrospray mass spectrometry.
abstract: Due to the broad impact of microfabrication technology on chemistry and biology, new methods to pattern and etch a variety of materials are being explored in a number of laboratories. Here, we report the design, fabrication, and operation of a glassy carbon (GC) microchip interfaced to a nanoelectrospray ionization source and a quadrupole mass spectrometer. The method involves standard photolithographic pattern transfer to a photoresist layer and anodization of the exposed GC substrate in basic electrolyte to produce a series of channels with well-defined wall structure. The performance of the microchip was evaluated with standard polymer and peptide samples.
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2 Canada.
authors.forename: Solomon
authors.initials: S
authors.lastname: Ssenyange
authors.forename: Justine
authors.initials: J
authors.lastname: Taylor
authors.forename: D Jed
authors.initials: DJ
authors.lastname: Harrison
authors.forename: Mark T
authors.initials: MT
authors.lastname: McDermott
mesh_terms: D002244:Carbon / Q000737:chemistry*; D004573:Electrolytes; D005898:Glass / Q000737:chemistry*; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D010455:Peptides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods*
chemical_list: D004573:Electrolytes; D010455:Peptides; D011108:Polymers; D002244:Carbon