title: Influence of clindamycin, metronidazole and polymyxin B on the expression of cell adhesion molecules stimulated by endotoxin and enterotoxin of Bacteroides fragilis.
abstract: The aim of this study was to compare the influence of antimicrobials (clindamycin, metronidazole and polymyxin B) on the expression of adhesion molecules (VCAM-1, ICAM-1 and E-selectin) on the HMEC-1 cell line stimulated by LPS and enterotoxin of B. fragilis. LPS was extracted from two reference: ATCC 43858 and NCTC 11295 and one isolated in our laboratory (W2) enterotoxigenic strains, and one nonenterotoxigenic reference strain--IPL E 323. Enterotoxin preparations (Tox 1 and Tox 2) were isolated from supematant of B. fragilis ATCC 43858 culture and purified. HMEC-1 cell line was stimulated with bacterial preparations at concentration of 10 mg/ml. For measuring the expression of adhesion molecules we used ELISA test. Clindamycin, metronidazole and polymyxin B supressed the ICAM-1 expression when endothelium was stimulated with B. fragilis LPS and augmented ICAM-1 expression by Tox 1 and Tox 2. The expression of VCAM-1 was augmented by antimicrobials when endothelium was stimulated with LPS or enterotoxin preparations. The expression of E-selectin was differentiated.
authors.affiliation: Department of Medical Microbiology, Medical University of Warsaw, Warsaw, Poland. fmikolajczyk@post.pl
authors.forename: Felicja
authors.initials: F
authors.lastname: Meisel-Mikołajczyk
authors.forename: Alicja
authors.initials: A
authors.lastname: Rokosz
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kot
authors.forename: Elwira
authors.initials: E
authors.lastname: Zawidzka
authors.forename: Cezary
authors.initials: C
authors.lastname: Malchar
authors.forename: Maria
authors.initials: M
authors.lastname: Nowaczyk
authors.forename: Andrzej
authors.initials: A
authors.lastname: Górski
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001441:Bacteroides fragilis / Q000378:metabolism*; D002460:Cell Line; D002981:Clindamycin / Q000494:pharmacology; D019040:E-Selectin / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism* / Q000382:microbiology; D004731:Endotoxins / Q000494:pharmacology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D008666:Metalloendopeptidases / Q000494:pharmacology; D008795:Metronidazole / Q000494:pharmacology; D011112:Polymyxin B / Q000494:pharmacology; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis*
chemical_list: D000900:Anti-Bacterial Agents; D019040:E-Selectin; D004731:Endotoxins; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D008795:Metronidazole; D002981:Clindamycin; D008666:Metalloendopeptidases; C091198:fragilysin; D011112:Polymyxin B