title: Preparation of highly active alpha-chymotrypsin for catalysis in organic media.
abstract: A simple one step process for the preparation of free alpha-chymotrypsin, using an organic solvent to precipitate the enzyme from a buffered solution, followed by washing with organic solvents, is described. This preparation gave 132 times greater esterification activity than lyophilized powder.
authors.affiliation: Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India.
authors.forename: Ipsita
authors.initials: I
authors.lastname: Roy
authors.forename: Munishwar N
authors.initials: MN
authors.lastname: Gupta
mesh_terms: D002384:Catalysis; D002918:Chymotrypsin / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D009930:Organic Chemicals / Q000737:chemistry*
chemical_list: D009930:Organic Chemicals; D002918:Chymotrypsin; C117608:alpha-chymotrypsin