Structural and biomolecular changes in aorta and pulmonary trunk of patients with aortic aneurysm and valve disease: implications for the Ross procedure.
OBJECTIVE
A higher incidence of pulmonary autograft dilatation is assumed in patients with ascending aortic dilatation and bicuspid aortic valve disease. To examine whether structural abnormalities are present in the ascending aorta as well as in the pulmonary trunk (PT) we specifically addressed molecular mechanisms and signalling pathways for aneurysm formation in ascending aortic aneurysms and PT of patients with different aortic valve pathology undergoing an extended Ross procedure.


METHODS
Wall segments resected from aortic aneurysms (20 patients, 7 bicuspid aortic valves BAV, and 13 tricuspid aortic valves TAV) and from PTs were submitted to analysis of leukocyte infiltration (immunohistochemistry), smooth muscle cell (SMC) apoptosis (in situ end-labelling of DNA-fragments TUNEL), and expression of death-promoting proteins perforin, granzyme B, Fas/FasL (immunoblotting).


RESULTS
Degenerative changes including rarefication and apoptosis of SMCs were significantly more severe in BAV than TAV disease (apoptotic index 9.2+/-3.2 vs. 11.9+/-6.2, P = 0.02). Immunohistochemistry confirmed presence and activation of death-promoting mediators in aneurysmal tissue whereas pulmonary tissue displayed only few apoptotic cells, occasional Fas+cells, rarely colocalized with FasL. By Western blot analysis extracts from BAV and TAV but not pulmonary artery wall contained appreciable amounts of perforin, granzyme B, and Fas/FasL.


CONCLUSIONS
Aneurysm formation is associated with SMC apoptosis and local signal expression of activated cells in patients with bicuspid as well as TAV. The PT itself is not pathologically involved with only minor degenerative changes. Although the disease process in the aorta appeared to be more severe in patients with BAV, there was similarity of histological and molecular changes of the pulmonary artery wall in all patients. Dilation of the pulmonary autograft seems not to be the result of histopathological and biomolecular mechanisms in the PT.
Schmid
Franz-Xaver
FX
Department of Cardiothoracic and Vascular Surgery, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, D-93053 Regensburg, Germany. fxschmid@klinik.uni-regensburg.de
Bielenberg
Katrin
K
Holmer
Stephan
S
Lehle
Karla
K
Djavidani
Behrus
B
Prasser
Christopher
C
Wiesenack
Christoph
C
Birnbaum
Dietrich
D
D000293:Adolescent; D000328:Adult; D000368:Aged; D001011:Aorta / Q000378:metabolism / Q000473:pathology*; D001014:Aortic Aneurysm / Q000150:complications / Q000473:pathology* / Q000601:surgery; D001021:Aortic Valve / Q000473:pathology; D017209:Apoptosis; D053804:Granzymes; D006349:Heart Valve Diseases / Q000150:complications / Q000473:pathology* / Q000601:surgery; D019918:Heart Valve Prosthesis Implantation; D006801:Humans; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000473:pathology; D011651:Pulmonary Artery / Q000378:metabolism / Q000473:pathology* / Q000637:transplantation; D012697:Serine Endopeptidases / Q000378:metabolism; D019014:fas Receptor / Q000378:metabolism
D019014:fas Receptor; C509213:GZMB protein, human; D053804:Granzymes; D012697:Serine Endopeptidases