Transplantation of bone marrow stromal cells into the cochlea of chinchillas.
This study aimed to evaluate the potential of bone marrow stromal cells for treatment of inner ear diseases. Autologous marrow cells labeled with Dil were implanted into the inner ear of five gentamicin-treated chinchillas. Histological analysis 3 weeks later revealed robust survival of grafted marrow cells in multiple regions within the cochlea. Marrow cells implanted in the basal turn of the cochlea migrated as far as the apical end or into the spiral ligament of the cochlea. Some grafted cells expressed a neuronal or glial cell marker, indicating their ability to differentiate into neuronal or glial cells. Survival, migrational mobility and differentiation of autologous marrow cells in damaged cochlea suggest their potential as transplants for treatment of various degenerative inner ear diseases.
Naito
Yasushi
Y
Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan. naito@ent.kuhp.kyoto-u.ac.jp
Nakamura
Tatsuo
T
Nakagawa
Takayuki
T
Iguchi
Fukuichiro
F
Endo
Tsuyoshi
T
Fujino
Kiyohiro
K
Kim
Tae-Soo
TS
Hiratsuka
Yasuyuki
Y
Tamura
Tetsuya
T
Kanemaru
Shin-ichi
S
Shimizu
Yoshihiko
Y
Ito
Juichi
J
D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods* / Q000473:pathology; D002465:Cell Movement / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002682:Chinchilla; D003051:Cochlea / Q000166:cytology* / Q000502:physiology; D013136:Spiral Ganglion / Q000166:cytology / Q000502:physiology; D017154:Stromal Cells / Q000637:transplantation