[Epitheliotrophic capacity of serum and plasma eyedrops. Influence of centrifugation].
BACKGROUND
Serum eyedrops are a new modality for the treatment of ocular surface disorders. We examined the influence of the preparation of blood products in a cell culture model and compared it with plasma.


METHODS
Serum and plasma were obtained from full blood of ten healthy volunteers and centrifuged at 500 and 3000 G. EGF, PDGF, TGF-beta1, fibronectin, and vitamin A were quantified by means of ELISA and HPLC. Cultures of human corneal epithelial cells were incubated with the four blood products in dose-response experiments and the intracellular ATP quantified.


RESULTS
EGF, PDGF, and vitamin A were present in serum in significantly higher concentrations than in plasma. The concentration of fibronectin was not influenced by the preparation. Support of proliferation was best by 25% platelet-poor serum. Serum supported the differentiation and migration of epithelial cells better than plasma.


CONCLUSIONS
The biochemical character of serum eyedrops is determined by the parameters chosen to produce the blood product. Plasma does not seem to offer an epitheliotrophic capacity equivalent to serum eyedrops. Their production should be optimized before any meaningful randomized controlled clinical trial can be attempted.
Herminghaus
P
P
Klinik für Augenheilkunde, Universitätsklinikum Schleswig-Holstein, Campus Lübeck.
Geerling
G
G
Hartwig
D
D
Wedel
T
T
Dibbelt
L
L
D000255:Adenosine Triphosphate / Q000378:metabolism; D001798:Blood Proteins / Q000737:chemistry / Q000494:pharmacology*; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects; D002498:Centrifugation; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D004815:Epidermal Growth Factor / Q000032:analysis / Q000494:pharmacology; D019573:Epithelium, Corneal / Q000187:drug effects*; D005353:Fibronectins / Q000032:analysis / Q000494:pharmacology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009883:Ophthalmic Solutions; D010976:Platelet Count; D010982:Platelet-Derived Growth Factor / Q000032:analysis / Q000494:pharmacology; D044967:Serum / Q000737:chemistry; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000494:pharmacology; D014801:Vitamin A / Q000032:analysis / Q000494:pharmacology
D001798:Blood Proteins; D005353:Fibronectins; D009883:Ophthalmic Solutions; D010982:Platelet-Derived Growth Factor; D016212:Transforming Growth Factor beta; D014801:Vitamin A; D004815:Epidermal Growth Factor; D000255:Adenosine Triphosphate