title: Comparison of cryopreserved homografts and decellularized porcine heterografts implanted in sheep.
abstract: This study evaluated cryopreserved homografts (Group 1) and porcine heterografts decellularized with deoxicholic acid (Group 2), implanted in the right ventricular outflow tract of juvenile sheep. Two groups with four animals in each were used and all animals survived with good outcome. Animals were sacrificed 90 or more days after surgery (90-150 days). On the third and fifth postoperative months they were submitted to echocardiographic examination with normal function and appearance observed for both groups. Explants were evaluated through histological analysis, atomic spectrophotometry and radiological examination. Calcium content was higher in the cusps of cryopreserved homografts, despite an otherwise similar macroscopic appearance between grafts of both groups. Decellularized heterografts were progressively repopulated by autologous cells suggesting some regenerative ability and longer durability than conventional homografts.
authors.affiliation: Cardiac Surgery Service of Aliança Saúde PUC-PR, Santa Casa de Curitiba, Paraná, Brazil. fcosta@mps.com.br
authors.forename: Francisco Diniz
authors.initials: FD
authors.lastname: Affonso da Costa
authors.forename: Pascal Maria
authors.initials: PM
authors.lastname: Dohmen
authors.forename: Sergio Veiga
authors.initials: SV
authors.lastname: Lopes
authors.forename: Guilherme
authors.initials: G
authors.lastname: Lacerda
authors.forename: Felipe
authors.initials: F
authors.lastname: Pohl
authors.forename: Ricardo
authors.initials: R
authors.lastname: Vilani
authors.forename: Marise Brenner
authors.initials: MB
authors.lastname: Affonso Da Costa
authors.forename: Eduardo Discher
authors.initials: ED
authors.lastname: Vieira
authors.forename: S
authors.initials: S
authors.lastname: Yoschi
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Konertz
authors.forename: Iseu
authors.initials: I
authors.lastname: Affonso da Costa
mesh_terms: D000818:Animals; D001705:Bioprosthesis; D002114:Calcinosis / Q000000981:diagnostic imaging; D002118:Calcium / Q000032:analysis; D015925:Cryopreservation; D005109:Extracellular Matrix / Q000502:physiology; D005347:Fibroblasts / Q000378:metabolism; D006350:Heart Valve Prosthesis; D011664:Pulmonary Valve / Q000601:surgery*; D011859:Radiography; D012756:Sheep; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D002118:Calcium