Interaction of catechins with aluminum in vitro.
Tea is one of the most popular beverages, consumed by over two thirds of the world's population; but the aluminum accumulation property of tea plant is becoming the focus of many researches because of aluminum's known adverse effect on human health. Investigation of the interactions of catechins with Al(3+) showed that during the interaction of catechins with Al(3+), the UV-vis spectrum of catechins was changed. Absorption of EGCG at 274 nm decreased and increased at 322 nm; EC and C's at 278 nm changed little. The ratio of Al(3+) to EGCG was 1:1 in pH 5.0 buffer solution; in pH 6.2 buffer solution, the ratio in the Al-EGCG complex was 1:1. Interestingly, while the ratio reached to over 2, after the complex of Al-EGCG started polymerization, the ratio in the polymer was 2:1. In pH 6.2 buffer solution, the complex behavior of C with Al(3+) was the same as that of EGCG, with a little difference for EC. When the ratio of Al(3+) to EC was <1, the complex in ratio was 1:2, but, the complex polymerized when the ratio of Al(3+) to EC was >1. It was found that the ratio of Al(3+) to EC in the polymer was 1:1. Polymerization of Al-catechin complexes might reduce aluminum absorption in the intestine. Kow value was also employed to study the properties of aluminum species in tea infusion (at gastric and intestine pH condition) and the effect of catechins and tea polyphenols on Kow in buffer solution. Results showed that Kow value rose much higher at the intestine pH than at the gastric pH. Tea polyphenols and catechins could greatly reduce aluminum Kow value in acetic buffer, indicating that these compounds may reduce aluminum absorption during tea intake.
Tang
De-song
DS
Department of Tea Sciences, Zhejiang University, Hangzhou 310029, China.
Shen
Sheng-rong
SR
Chen
Xun
X
Zhang
Yu-yan
YY
Xu
Chong-yang
CY
D019342:Acetic Acid; D000535:Aluminum / Q000737:chemistry*; D002021:Buffers; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry; D005459:Fluorides / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols; D013057:Spectrum Analysis; D013662:Tea / Q000737:chemistry*
D002021:Buffers; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate; D000535:Aluminum; D019342:Acetic Acid; D005459:Fluorides