Bacterial bioabsorption of nickel from industrial cooling water.
Three bacterial strains tolerant to the presence of 100 mg litre(-1) nickel ions were isolated from a water reclamation system. Each organism was tested for ability to accumulate nickel at the above-mentioned concentration. The organism capable of maximum nickel accumulation was identified as an Enterobacter sp. and intracellular nickel deposition by this microorganism was determined using energy dispersive X-ray analysis (EDAX) and transmission electron microscopy. A metal-staining technique for light microscopy was developed. Further studies revealed that growth and glucose utilisation by this isolate was inhibited in culture by nickel.
Kasan
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HC
Department of Microbiology, University of Witwatersrand, 1 Jan Smuts avenue, Johannesburg, 2000, South Africa.
Stegmann
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