title: [Influence of sodium butyrate on growth of HT-29 colon carcinoma cells and expression of inducible nitric oxide synthase (iNOS)].
abstract: OBJECTIVE
  Sodium butyrate could inhibit several cancer cell lines in vitro. This study was designed to investigate the effects of sodium butyrate on the growth of HT-29 colon carcinoma cells, the expression of iNOS, and the excretion of NO.
  
  
  METHODS
  The HT-29 cells were co-incubated with sodium butyrate at various concentration. The cell proliferation was determined by MTT test, and the iNOS protein of HT-29 cells was stained by immunochemical SP method. The absorbance value (A value) of iNOS was measured by automatic image analysis system. Griess's technique was used to examine the excretion of nitric oxide (NO).
  
  
  RESULTS
  The survival rates of HT-29 cells treated by sodium butyrate decreased in a time-dependent and dose-dependent manner. IC(50) value of sodium butyrate was decreased with prolonged incubation time (15.4 mmol/L at 12 hours, 5.7 mmol/L at 24 hours, 2.5 mmol/L at 36 hours, 0.9 mmol/L at 48 hours). Simultaneously sodium butyrate decreased the expression of iNOS in the cytoplasm and the excretion of NO in a similar manner.
  
  
  CONCLUSIONS
  Sodium butyrate could inhibit the expression of iNOS, consequently reduce the excretion of NO. It may play an important role in the mechanism that sodium butyrate inhibits the growth of HT-29 colon carcinoma cells.
authors.affiliation: Department of Gastroenterology, Renming Hospital, Wuhan University, Wuhan, Hubei, 430060, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Li
authors.forename: He-Sheng
authors.initials: HS
authors.lastname: Luo
authors.forename: Yi-Juan
authors.initials: YJ
authors.lastname: Ding
authors.forename: Xi
authors.initials: X
authors.lastname: Li
mesh_terms: D002087:Butyrates / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D019073:HT29 Cells / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D007150:Immunohistochemistry; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000032:analysis*; D052247:Nitric Oxide Synthase Type II
chemical_list: D002087:Butyrates; D009569:Nitric Oxide; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II