A preliminary study on DNA detection based on relative magnetic permeability measurements and histone H1 conjugated superparamagnetic nanoparticles as magnetic tracers.
Histone H1 conjugated superparamagnetic nanoparticles were assessed for their ability to work as magnetic tracers in conjunction with the relative magnetic permeability metre (MPM-100) for the detection and quantification of DNA (deoxyribonucleic acid). The method employed was based on the electrostatic adsorption of DNA (analyte) to amino group derivatised silica (carrier) and subsequent binding of histone H1 conjugated superparamagnetic nanoparticles (magnetic tracer). The sandwich complexes formed were separated from the medium by sedimentation and the relative magnetic permeability of the sediments were measured with the MPM-100. Investigations were made with both calf thymus DNA and plasmid DNA in aqueous buffered solution as well as in a lysed cell culture with high protein content. For the quantification of calf thymus DNA, a linear relationship between the DNA concentration in the sample and the relative magnetic permeability of the pellet was found for DNA concentrations up to 67 microg/ml in buffered solutions as well as in a lysed cell culture. The limits of detection were determined to 12 and 31 microg/ml, respectively. For the quantification of plasmid DNA in buffered solution a linear range was established for concentrations in up to 150 microg/ml and the limit of detection was determined to 52 microg/ml.
Abrahamsson
Daniel
D
Department of Pure and Applied Biochemistry, Centre for Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
Kriz
Kirstin
K
Lu
Min
M
Kriz
Dario
D
D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis*; D006657:Histones; D007700:Kinetics; D008280:Magnetics; D043942:Nanotubes; D012822:Silicon Dioxide
D006657:Histones; D012822:Silicon Dioxide; D004247:DNA