Cross-clade HIV-1 neutralization by an antibody fragment from a lupus phage display library.
A single-chain fragment containing antibody V domains (scFv) isolated from a lupus antibody library displayed the ability to bind gp120 and the conserved gp120 determinant composed of residues 421-436. The scFv neutralized R5 and X4-dependent HIV-1 strains from clades B, C, and D. The lupus repertoire may be useful as a source of neutralizing antibodies to HIV.
Karle
Sangeeta
S
Chemical Immunology Research Center, Department of Pathology, University of Texas, Houston Medical School, USA.
Planque
Stephanie
S
Nishiyama
Yasuhiro
Y
Taguchi
Hiroaki
H
Zhou
Yong-Xin
YX
Salas
Maria
M
Lake
Douglas
D
Thiagarajan
Perumal
P
Arnett
Frank
F
Hanson
Carl V
CV
Paul
Sudhir
S
D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000378:metabolism*; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007128:Immunoglobulin Fragments / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology
D000939:Epitopes; D015699:HIV Envelope Protein gp120; D007128:Immunoglobulin Fragments