Dual-Color imaging of nuclear division and mitotic spindle elongation in live cells of Aspergillus nidulans.
We have developed a dual-color imaging system based on cyan fluorescent protein-labeled histone H2A and green fluorescent protein-labeled alpha tubulin to visualize DNA and spindles simultaneously in the same living cell of Aspergillus nidulans. This system allows new details of mitosis and nuclear movement to be revealed.
Su
Wenqi
W
Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA.
Li
Shihe
S
Oakley
Berl R
BR
Xiang
Xin
X
D001233:Aspergillus nidulans / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure*; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure*; D020090:Chromosome Segregation; D003116:Color; D004247:DNA / Q000737:chemistry; D008856:Microscopy, Fluorescence / Q000379:methods*; D008941:Spindle Apparatus / Q000378:metabolism / Q000648:ultrastructure*
D004247:DNA