title: Different effects of cytochalasins on the growth and differentiation of Entamoeba invadens.
abstract: The effect of five different cytochalasins on the growth, encystation and excystation of Entamoeba invadens was examined. At 10 microM, cytochalasins B, D, E and dihydrocytochalasin B markedly inhibited growth. Encystation was inhibited by cytochalasin D at 1 microM but not by other cytochalasins at the same concentration, whereas it was inhibited by 10 microM of cytochalasins B, E and dihydrocytochalasin B as well as cytochalasin D. Excystation, which was assessed by counting the number of metacystic amoebae after inducing excystation, was markedly enhanced by cytochalasin D as previously demonstrated, whereas the enhancing effect of cytochalasins A, B and dihydrocytochalasin B was weak. In contrast, cytochalasin E at 10 microM inhibited excystation and metacystic development. These results indicate that there is a difference in the effect of different cytochalasins on the growth and differentiation of E. invadens, depending on differences in their chemical structure.
authors.affiliation: Department of Tropical Medicine, Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan. makioka@jikei. ac. jp
authors.forename: Asao
authors.initials: A
authors.lastname: Makioka
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kumagai
authors.forename: Seiki
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Takeuchi
mesh_terms: D000818:Animals; D003470:Culture Media; D003572:Cytochalasins / Q000737:chemistry / Q000494:pharmacology*; D004747:Entamoeba / Q000187:drug effects* / Q000254:growth & development* / Q000502:physiology
chemical_list: D003470:Culture Media; D003572:Cytochalasins