Regional differences in blood-nerve barrier function and tight-junction protein expression within the rat dorsal root ganglion.
To elucidate blood-nerve barrier function and tight-junction protein expression in the dorsal root ganglion (DRG), we analyzed the vascular permeability in the rat DRG by i.v. administration of fluorescent Evans-blue albumin (EBA) and compared it with the localization of claudin-1, claudin-5, and occludin by immunoconfocal microscopy. In the cell body-rich area within the DRG, extravascular leakage of EBA was noted and claudin-5 but neither claudin-1 nor occludin was detected. Conversely, in the nerve fiber-rich area within the DRG, no extravascular leakage of EBA was observed and both claudin-5 and occludin but no claudin-1 were detected in the blood vessel. These results demonstrate regional differences in the blood-nerve barrier function and tight-junction protein expression within the DRG.
Hirakawa
Hisanori
H
Department of Pathology and Cell Regulation, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji, Kamigyo-ku, Kyoto, 602-8566, Japan.
Okajima
Seiichiro
S
Nagaoka
Takanori
T
Kubo
Toshikazu
T
Takamatsu
Tetsuro
T
Oyamada
Masahito
M
D000818:Animals; D002199:Capillary Permeability / Q000502:physiology; D062445:Claudin-1; D062507:Claudin-5; D005070:Evans Blue; D005456:Fluorescent Dyes; D005727:Ganglia, Spinal / Q000378:metabolism* / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D018613:Microscopy, Confocal; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D062793:Occludin; D051381:Rats; D017208:Rats, Wistar; D019108:Tight Junctions / Q000378:metabolism* / Q000502:physiology*; D014841:von Willebrand Factor / Q000096:biosynthesis / Q000235:genetics
D062445:Claudin-1; D062507:Claudin-5; C568114:Cldn1 protein, rat; C491510:Cldn5 protein, rat; D005456:Fluorescent Dyes; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D062793:Occludin; C568987:Ocln protein, rat; D014841:von Willebrand Factor; D005070:Evans Blue