Determination of Ochratoxin A in small volumes of human blood serum.
A new simple and rapid method for analysing Ochratoxin A (OTA) in small volumes of human blood serum using capillary zone electrophoresis coupled to laser-induced fluorescence is described. The clean-up procedure solely consists of a double extraction step. To improve the reproducibility of migration times and quantification, two internal standards were used. The limit of detection was 0.55 ng/ml, with a linear range of 1-100 ng/ml of OTA in spiked human blood serum. The method is used to rapidly screen suspected patients.
Köller
G
G
Department of Human Exposure Research and Epidemiology, Centre for Environmental Research Leipzig-Halle, Permoserstr. 15, 04318 Leipzig, Germany. gabor.koeller@ufz.de
Rolle-Kampczyk
U
U
Lehmann
I
I
Popp
P
P
Herbarth
O
O
D006801:Humans; D009183:Mycotoxins / Q000097:blood*; D009793:Ochratoxins / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A