title: Tuberculosis survey of free-ranging marsh deer (Blastocerus dichotomus) in Brazil.
abstract: Esophageal-pharyngeal fluids from 53 free-ranging marsh deer (Blastocerus dichotomus) captured for a research program in the state of Mato Grosso do Sul, Brazil, were assayed for tuberculosis. Total DNA was extracted. amplified by polymerase chain reaction using specific primers for Mycobacterium tuberculosis complex (M. tuberculosis, M. bovis, M. microti, and M. africanum), and observed by agarose gel electrophoresis stained with ethidium bromide. All samples were negative. This, along with necropsy and histopathology data, suggests that these animals are not shedding and probably do not have active disease.
authors.affiliation: Laboratório de Biologia Molecular Aplicada e Sorologia, Departamento de Medicina Veterinária Preventiva, Universidade de São Paulo, Av. Orlando Marques de Paiva, 87, São Paulo City, São Paulo, Brazil.
authors.forename: Janaina O
authors.initials: JO
authors.lastname: Luna
authors.forename: Manoel A A
authors.initials: MA
authors.lastname: Santos
authors.forename: Edison L
authors.initials: EL
authors.lastname: Durigon
authors.forename: João P
authors.initials: JP
authors.lastname: Araújo Júnior
authors.forename: José M B
authors.initials: JM
authors.lastname: Duarte
mesh_terms: D000818:Animals; D000835:Animals, Wild; D001938:Brazil / Q000453:epidemiology; D004269:DNA, Bacterial / Q000032:analysis*; D003670:Deer / Q000382:microbiology*; D009161:Mycobacterium / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D014376:Tuberculosis / Q000453:epidemiology / Q000662:veterinary*
chemical_list: D004269:DNA, Bacterial