Glucose-induced expression of carotenoid biosynthesis genes in the dark is mediated by cytosolic ph in the cyanobacterium Synechocystis sp. PCC 6803.
The expression of carotenoid biosynthesis genes coding for phytoene synthase (crtB), phytoene desaturase (crtP), zeta-carotene desaturase (crtQ), and beta-carotene hydroxylase (crtR) is dependent upon light in the cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis). We have demonstrated that the expression of the above four genes was also elevated in the dark-adapted Synechocystis cells upon glucose treatment as a consequence of transcriptional activation. Treatment with glucose analogs such as l-glucose, 3-O-methylglucose, 2-deoxyglucose, and mannose, or inactivation of glucose uptake and phosphorylation by deletion mutation of glucose transporter (glcP) and glucokinase (gk), respectively, did not induce up-regulation of carotenoid genes. When respiratory electron transport or coupling to oxidative phosphorylation was inhibited, glucose induction was not observed, indicating that respiratory electron transport per se is not critical for the expression of these genes. In agreement with this view, the extent of gene expression showed a saturation curve with increasing acridine yellow fluorescence yield, without having a close correlation with the ATP contents or ATP/ADP ratio. The results indicate that glucose induction of carotenoid gene expressions is mediated by an increase in cytosolic pH rather than either redox or glucose sensing.
Ryu
Jee-Youn
JY
Department of Biology, Chungnam National University, Daejeon 305-764, Korea.
Song
Ji Young
JY
Lee
Jeong Mi
JM
Jeong
Suk Won
SW
Chow
Wah Soon
WS
Choi
Sang-Bong
SB
Pogson
Barry J
BJ
Park
Youn-Il
YI
D002338:Carotenoids / Q000096:biosynthesis*; D000458:Cyanobacteria / Q000235:genetics* / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism*; D004579:Electron Transport; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D008027:Light; D010085:Oxidative Phosphorylation; D011190:Potassium Cyanide / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis
D012333:RNA, Messenger; D002338:Carotenoids; D005947:Glucose; D011190:Potassium Cyanide