title: Renal ischemia induces tropomyosin dissociation-destabilizing microvilli microfilaments.
abstract: Ischemic-induced cell injury results in rapid duration-dependent actin-depolymerizing factor (ADF)/cofilin-mediated disruption of the apical microvilli microfilament cores. Because intestinal microvillar microfilaments are bound and stabilized in the terminal web by the actin-binding protein tropomyosin, we questioned whether a protective effect of tropomyosin localization to the terminal web of the proximal tubule microfilament cores is disrupted during ischemic injury. With tropomyosin-specific antibodies, we examined rat cortical sections under physiological conditions and following ischemic injury by confocal microscopy. In addition, Western blot analysis of cortical extracts and urine was undertaken. Our studies demonstrated the presence of tropomyosin isoforms in the proximal tubule microvillar terminal web under physiological conditions and their dissociation in response to 25 min of ischemic injury. This correlated with the excretion of tropomyosin-containing plasma membrane vesicles in urine from ischemic rats. In addition, we noted increased tropomyosin Triton X-100 solubility following ischemia in cortical extracts. These studies suggest tropomyosin binds to and stabilizes the microvillar microfilament core in the terminal web under physiological conditions. With the onset of ischemic injury, we propose that tropomyosin dissociates from the microfilament core providing access to microfilaments in the terminal web for F-actin binding, severing and depolymerizing actions of ADF/cofilin proteins.
authors.affiliation: Division of Nephrology, Department of Medicine, Indiana University, Indianapolis, IN 46202-5116, USA.
authors.forename: Sharon L
authors.initials: SL
authors.lastname: Ashworth
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Wean
authors.forename: Silvia B
authors.initials: SB
authors.lastname: Campos
authors.forename: Constance J
authors.initials: CJ
authors.lastname: Temm-Grove
authors.forename: Erica L
authors.initials: EL
authors.lastname: Southgate
authors.forename: Bernadette
authors.initials: B
authors.lastname: Vrhovski
authors.forename: Peter
authors.initials: P
authors.lastname: Gunning
authors.forename: Ron P
authors.initials: RP
authors.lastname: Weinberger
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Molitoris
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D051339:Actin Depolymerizing Factors; D000199:Actins / Q000378:metabolism; D000818:Animals; D051345:Destrin; D007511:Ischemia / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D016350:Leucine Zippers / Q000502:physiology; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism; D008871:Microvilli / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014335:Tropomyosin / Q000378:metabolism*; D014556:Urine
chemical_list: D051339:Actin Depolymerizing Factors; D000199:Actins; D051345:Destrin; D008840:Microfilament Proteins; D014335:Tropomyosin