Use of genome-scale microbial models for metabolic engineering.
Metabolic engineering serves as an integrated approach to design new cell factories by providing rational design procedures and valuable mathematical and experimental tools. Mathematical models have an important role for phenotypic analysis, but can also be used for the design of optimal metabolic network structures. The major challenge for metabolic engineering in the post-genomic era is to broaden its design methodologies to incorporate genome-scale biological data. Genome-scale stoichiometric models of microorganisms represent a first step in this direction.
Patil
Kiran Raosaheb
KR
Center for Process Biotechnology, Biocentrum-DTU, Building 223, Technical University of Denmark, DK-2800 Lyngby, Denmark.
Akesson
Mats
M
Nielsen
Jens
J
D000465:Algorithms; D001419:Bacteria / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D003198:Computer Simulation; D020869:Gene Expression Profiling / Q000379:methods; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D005818:Genetic Engineering / Q000379:methods*; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods; D008954:Models, Biological; D008956:Models, Chemical; D011994:Recombinant Proteins / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*
D001426:Bacterial Proteins; D011994:Recombinant Proteins