Hyphantria cunea ferritin heavy chain homologue: cDNA sequence and mRNA expression.
We have sequenced a cDNA clone encoding a 26-kDa ferritin subunit, which was heavy chain homologue (HCH), in fall webworm, Hyphantria cunea. The HCH cDNA was obtained from the screening of a cDNA library using a PCR product. H. cunea ferritin is composed of 221 amino acid residues and their calculated mass is 26,160 Da. The protein contains the conserved motifs for the ferroxidase center typical for heavy chains of vertebrate ferritin. The iron-responsive element sequence with a predicted stem-loop structure is present in the 5'-untranslated region of ferritin HCH mRNA. The sequence alignment of ferritin HCH shows 68.9 and 68.7% identity with Galleria mellonella HCH (26 kDa ferritin) and Manduca sexta HCH, respectively. While G type insect ferritin vertebrate light chain homologue (LCH) is distantly related to H. cunea ferritin HCH (17.2-20.8%), the Northern blot analysis revealed that H. cunea ferritin HCH was ubiquitously expressed in various tissues and all developmental stages. The ferritin expression of midgut is more responsive to iron-fed, compared to fat body in H. cunea.
Kim
Hong Ja
HJ
Division of Life Science, Gyeongsang National University, Jinju, Korea.
Yun
Chi Young
CY
Cheon
Hyang Mi
HM
Chae
Boa
B
Lee
In Hee
IH
Park
Seun Ja
SJ
Kang
Young Jin
YJ
Seo
Sook Jae
SJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics*; D005293:Ferritins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D015870:Gene Expression; D019476:Insect Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010802:Phylogeny; D021122:Protein Subunits; D012333:RNA, Messenger / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D019476:Insect Proteins; D021122:Protein Subunits; D012333:RNA, Messenger; D011994:Recombinant Proteins; D005293:Ferritins