[Study of injury model in human umbilical vein endothelial cells].
OBJECTIVE
To establishment the injury by oxidized low density lipoprotein in human umbilical vein endothelial cells.


METHODS
Huvecs were exposed to OX-LDL in vitro, then MTT and MDA of Huvecs were measured.


RESULTS
After endothelium cells were exposed to different dose of OX-LDL 24 hours, MTT was decreased significantly, especially in the high dose OX-LDL group.


CONCLUSIONS
OX-LDL could lead injury to Huvecs and might be related to atherosclerosis.
Liu
Fei
F
Institute of Health and Enviornment Medicine, Academy of Military Medical Sciences, Tianjin 300050, China.
Chang
Hong
H
Xie
Zhenyuan
Z
D001161:Arteriosclerosis / Q000209:etiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000473:pathology*; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008315:Malondialdehyde / Q000032:analysis; D014471:Umbilical Veins / Q000473:pathology
D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D008315:Malondialdehyde