Disruption of type 5 adenylyl cyclase negates the developmental increase in Galphaolf expression in the striatum.
The two stimulatory G protein alpha subunits, Galphas and Galphaolf, activate adenylyl cyclase in a similar way. We examined whether type 5 adenylyl cyclase knockout, the major striatal isoform, can differentially and/or developmentally change the expression of these G proteins in the striatum. Galphas and Galphaolf expressions at birth were unaffected in knockouts, which, however, demonstrated a blunted developmental increase in Galphaolf, but not Galphas. Adenylyl cyclase activity was unaffected at birth, but subsequently became lower in knockouts. These findings suggest that type 5 adenylyl cyclase does not contribute to striatal cAMP signaling at birth. However, it may play an important role in developmental changes in the expression of Galphaolf, but not Galphas.
Iwamoto
Tamio
T
Departments of Physiology and Medicine, Yokohama City University School of Medicine, Yokohama 234-0002, Japan.
Iwatsubo
Kousaku
K
Okumura
Satoshi
S
Hashimoto
Yoko
Y
Tsunematsu
Takashi
T
Toya
Yoshiyuki
Y
Hervé
Denis
D
Umemura
Satoshi
S
Ishikawa
Yoshihiro
Y
D000262:Adenylyl Cyclases / Q000235:genetics / Q000502:physiology*; D000367:Age Factors; D000818:Animals; D003342:Corpus Striatum / Q000378:metabolism*; D044385:GTP-Binding Protein alpha Subunits / Q000096:biosynthesis; D018507:Gene Expression Regulation, Developmental; D020962:Heterotrimeric GTP-Binding Proteins / Q000096:biosynthesis*; D007527:Isoenzymes / Q000235:genetics / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D015398:Signal Transduction
D044385:GTP-Binding Protein alpha Subunits; D007527:Isoenzymes; C084618:olfactory G protein subunit alpha olf; D020962:Heterotrimeric GTP-Binding Proteins; D000262:Adenylyl Cyclases; C419510:adenylyl cyclase type V