Ca(v)3.2 calcium channels control an autocrine mechanism that promotes neuroblastoma cell differentiation.
Calcium influx via low-voltage activated alpha(1H) (Ca(v)3.2) T-currents participates in the morphological and electrical differentiation of neuroblastoma NG108-15 cells. We investigated whether an autocrine mechanism could contribute to this differentiation process. The presence of factors secreted by NG108-15 cells was identified through the use of conditioned media (CM) obtained from differentiated cells. These CM significantly increased neuritogenesis with no change in the HVA calcium channel expression. CM-induced neuritogenesis persists during alpha(1H) current block, whereas CM obtained from cells transfected with an alpha(1H) antisense did not induce neuritogenesis. These data indicate that morphological differentiation of NG108-15 cells depends on an autocrine mechanism, which is controlled by alpha(1H) currents. Such a mechanism is likely to play a role in the various differentiation processes that imply alpha(1H) T-type Ca(2+) channels.
Chemin
Jean
J
Laboratoire de Génomique Fonctionnelle, UPR 2580 CNRS, Institut de Génétique Humaine, 141 rue de la Cardonille, F-34094 Montpellier cedex 05, France.
Nargeot
Joël
J
Lory
Philippe
P
D000818:Animals; D019898:Autocrine Communication / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D020747:Calcium Channels, T-Type / Q000235:genetics / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D045744:Cell Line, Tumor; D017077:Culture Media, Conditioned / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects / Q000235:genetics; D051379:Mice; D016501:Neurites / Q000187:drug effects / Q000378:metabolism; D009447:Neuroblastoma / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D020320:Oligoribonucleotides, Antisense; D018408:Patch-Clamp Techniques; D051381:Rats; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
C479952:CACNA1H protein, human; D020747:Calcium Channels, T-Type; D017077:Culture Media, Conditioned; D020320:Oligoribonucleotides, Antisense