A supramolecular approach to protein labeling. A novel fluorescent bioassay for concanavalin a activity.
[reaction: see text] A new method for the bioassay of concanavalin A is based on the interaction of saccharide-substituted oligopyrrolic macrocycles with lectins. A general sensing mechanism involves the interaction of aggregated and primarily nonfluorescent labels with the target protein, label deaggregation, site-specific binding, and fluorescence signaling. Addition of saccharides to the fluorescent lectin-macrocycle complex leads to the release of the label and partial quenching of fluorescence due to reaggregation. Specificity of the protein-probe binding is discussed.
Rusin
Oleksandr
O
Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803, USA.
Král
Vladimír
V
Escobedo
Jorge O
JO
Strongin
Robert M
RM
D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods*; D003208:Concanavalin A / Q000032:analysis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry; D008024:Ligands; D046911:Macromolecular Substances; D015394:Molecular Structure; D009005:Monosaccharides / Q000737:chemistry; D011166:Porphyrins / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D037102:Lectins; D008024:Ligands; D046911:Macromolecular Substances; D009005:Monosaccharides; D011166:Porphyrins; D011506:Proteins; D011758:Pyrroles; C440448:sapphyrin; D003208:Concanavalin A