title: The fission yeast ptr1+ gene involved in nuclear mRNA export encodes a putative ubiquitin ligase.
abstract: Fission yeast ptr1-1 is one of the mRNA transport mutants that accumulate poly(A)+ RNA in the nuclei at the nonpermissive temperature. We found that the ptr1+ gene encodes a homolog of Saccharomyces cerevisiae Tom1p, a hect type ubiquitin ligase. In ptr1-1, a conserved amino acid in the hect domain of Ptr1p is mutated. The ptr1+ gene is essential for growth and its mutation did not affect nuclear protein export. A ptr1-1 rae1-167 double mutant showed a synthetic effect on a growth defect, indicating that Ptr1p functionally interacts with an essential mRNA export factor Rae1p. We also isolated a multi-copy suppressor for ptr1-1 and found that it is the mpd2+ gene isolated as a multi-copy suppressor of cdc7-PD1.
authors.affiliation: Department of Biological Science, Faculty of Science, Kumamoto University, Kurokami, Kumamoto 860-8555, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Andoh
authors.forename: Abul Kalam
authors.initials: AK
authors.lastname: Azad
authors.forename: Asako
authors.initials: A
authors.lastname: Shigematsu
authors.forename: Yasumi
authors.initials: Y
authors.lastname: Ohshima
authors.forename: Tokio
authors.initials: T
authors.lastname: Tani
mesh_terms: D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000378:metabolism*; D003001:Cloning, Molecular; D003360:Cosmids / Q000235:genetics; D004171:Diploidy; D016153:Genes, Suppressor; D018398:Homeodomain Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D044767:Ubiquitin-Protein Ligases / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018398:Homeodomain Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C115246:TOM1 protein, S cerevisiae; D044767:Ubiquitin-Protein Ligases