title: Isolation and characterization of two distinct growth hormone cDNAs from the tetraploid smallmouth buffalofish (Ictiobus bubalus).
abstract: The growth hormone (GH) gene has been characterized for a number of fishes and used to establish phylogenetic relationships and population structures. Analysis of tetraploid fishes, such as salmon and some Asian cyprinids, has shown the presence of two GH genes. Fishes in the sucker family (Catostomidae, Cypriniformes) are also tetraploid, and the present study reports the isolation and characterization of two GH cDNAs from a representative species, the smallmouth buffalofish (Ictiobus bubalus). The GH cDNAs of smallmouth buffalofish are 1272 and 1273nt in length, and each codes for a polypeptide of 210 amino acids, predicted to be cleaved to a final product of 188 aa. The GH cDNAs of smallmouth buffalofish are 6% divergent in nt sequence in the coding region, and there are 16 differences in predicted aa sequence. Because the cDNAs have distinct sequences in coding regions and in UTRs, which differed by more than 10%, they were identified as GHI and GHII. The predicted GHI protein contains 4 Cys residues, homologous to other vertebrate GH sequences. On the other hand, GHII has 5 Cys residues, homologous to other ostariophysan sequences. GHI and GHII are most similar to other cypriniform fishes for both nt and protein sequences. Phylogenetically, the sequences of smallmouth buffalofish GH consistently grouped with Asian cyprinids, but not loaches, consistent with morphological evidence suggesting that suckers are most closely related to minnows.
authors.affiliation: Department of Biochemistry, Tulane University School of Medicine, 1430 Tulane Avenue, New Orleans, LA 70112-2699, USA.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Clements
authors.forename: Henry L
authors.initials: HL
authors.lastname: Bart
authors.forename: David L
authors.initials: DL
authors.lastname: Hurley
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003531:Cypriniformes / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D013006:Growth Hormone / Q000235:genetics*; D008969:Molecular Sequence Data; D011123:Polyploidy; D020033:Protein Isoforms; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D020033:Protein Isoforms; D012313:RNA; D013006:Growth Hormone