Seasonal environmental changes modulate the prolactin receptor expression in an eurythermal fish.
Eurythermal fish have evolved compensatory responses to the cyclical seasonal changes of the environment. The complex adaptive mechanisms include the transduction of the physical parameters variations into molecular signals. Studies in carp have indicated that prolactin and growth hormone expression is associated with acclimatization, suggesting that the pituitary gland is a relevant physiological node in the generation of the homeostatic rearrangement that occurs in this adaptive process. Here, we report the cloning and characterization of a full-length carp prolactin receptor cDNA, which codes for the long form of the protein resembling that found in mammalian prolactin receptors. We identified up to three receptor transcript isoforms in different tissues of the teleost and assessed cell- and temporal-specific transcription and protein expression in carp undergoing seasonal acclimatization. The distinctive pattern of expression that carp prolactin receptor (cPRLr) depicts upon seasonal acclimatization supports the hypothesis that prolactin and its receptor are clearly involved in the new homeostatic stage that the eurythermal fish needs to survive during the cyclical changes of its habitat.
San Martín
Rody
R
Millenium Institute for Fundamental and Applied Biology, Universidad Andrés Bello, Santiago, Chile.
Cáceres
Pilar
P
Azócar
Rodrigo
R
Alvarez
Marco
M
Molina
Alfredo
A
Vera
María I
MI
Krauskopf
Manuel
M
D000064:Acclimatization / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002347:Carps / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D005880:Gills / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D011981:Receptors, Prolactin / Q000096:biosynthesis / Q000235:genetics*; D012621:Seasons
D012333:RNA, Messenger; D011981:Receptors, Prolactin