Islet film cures diabetes.
Intraportal transplantation of islets is usually performed by a percutaneous, transhepatic catheterization technique for type 1 diabetic patients. We show here an easier method of islet implantation into the liver. An islet film was made using rat islet and was simply placed on the liver of a diabetic recipient rat. Histologic examinations revealed that the islets passed through the liver capsule within a week after the implantation, entered the liver parenchyma within 3 weeks, and were encircled with hepatocytes by day 222. Recipient rats were cured of diabetes; however nonimplanted control rats had high levels of blood glucose. The film-transplanted islets could provide adequate insulin to maintain stable blood glucose levels. This novel method requires little skill and no special instruments. This islet film approach may prove suitable for simple islet transplantation in clinical cases.
Yamasaki
Toru
T
Department of Surgery and Surgical Basic Science, Graduate School of Medicine, Kyoto University, 54 Kawaracho, Shogoin, Sakyo-ku, 606-8507, Kyoto, Japan. toru@kuhp.kyoto-u.ac.jp
Onodera
Hisashi
H
Takahashi
Rei
R
Imamura
Masayuki
M
D000818:Animals; D001673:Biodegradation, Environmental; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008297:Male; D051381:Rats; D011912:Rats, Inbred ACI