title: The capillary electrophoresis separation of benzodiazepine drug using dextran sulfate and SDS as running buffer.
abstract: Capillary electrophoresis has been applied the analyses of many clinical drugs due to its rapid, high-resolution separation. In this study, electrokinetic chromatography involving the combination of SDS and dextran sulfate, which are synthetic polymers, was examined in order to obtain high resolution. Use of 2% dextran sulfate (10,000 molecular weight), 20 mm SDS running buffer containing boric acid solution (pH 9.2) and a silica capillary (inner diameter of 75 micro m, effective length of 50 cm, 57 cm overall length) afforded separation of 10 kinds of benzodiazepines. The detection limit was 0.2 micro g/mL; additionally, reproducibilities were de fi ned as the peak height and migration time. The average peak height was 5.92% (2.46-17.61), whereas the average migration time was 0.44% (0.18-0.76; n = 5). This separations system can be applied to the analysis and measurement of other pharmaceuticals as well.
authors.affiliation: School of Pharmaceutical Sciences, Showa University, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Arakawa
authors.forename: Masako
authors.initials: M
authors.lastname: Maeda
mesh_terms: D001569:Benzodiazepines / Q000302:isolation & purification*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D016264:Dextran Sulfate / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*
chemical_list: D001569:Benzodiazepines; D012967:Sodium Dodecyl Sulfate; D016264:Dextran Sulfate