Effect of acetic acid present in bagasse hydrolysate on the activities of xylose reductase and xylitol dehydrogenase in Candida guilliermondii.
The first two steps in xylose metabolism are catalyzed by NAD(P)H-dependent xylose reductase (XR) (EC 1.1.1.21) and NAD(P)-dependent xylitol dehydrogenase (XDH) (EC 1.1.1.9), which lead to xylose-->xylitol-->xylulose conversion. Xylitol has high commercial value, due to its sweetening and anticariogenic properties, as well as several clinical applications. The acid hydrolysis of sugarcane bagasse allows the separation of a xylose-rich hemicellulosic fraction that can be used as a substrate for Candida guilliermondii to produce xylitol. However, the hydrolysate contains acetic acid, an inhibitor of microbial metabolism. In this study, the effect of acetic acid on the activities of XR and XDH and on xylitol formation by C. guilliermondii were studied. For this purpose, fermentations were carried out in bagasse hydrolysate and in synthetic medium. The activities of XR and XDH were higher in the medium containing acetic acid than in control medium. Moreover, none of the fermentative parameters were significantly altered during cell culture. It was concluded that acetic acid does not interfere with xylitol formation since the increase in XR activity is proportional to XDH activity, leading to a greater production of xylitol and its subsequent conversion to xylulose.
Lima
Luanne Helena Augusto
LH
Departamento de Biologia Celular, Universidade de Brasília, 70910-900 Brasilia, DF, Brazil.
das Graças de Almeida Felipe
Maria
M
Vitolo
Michele
M
Torres
Fernando Araripe Gonçalves
FA
D019342:Acetic Acid / Q000378:metabolism / Q000494:pharmacology*; D000449:Aldehyde Reductase / Q000378:metabolism*; D001089:Arabinose / Q000378:metabolism; D002175:Candida / Q000201:enzymology* / Q000254:growth & development; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry; D050542:D-Xylulose Reductase; D004790:Enzyme Induction; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005285:Fermentation; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism*; D014993:Xylitol / Q000378:metabolism; D014994:Xylose / Q000378:metabolism
D003470:Culture Media; D004791:Enzyme Inhibitors; D002482:Cellulose; C027433:bagasse; D014994:Xylose; D001089:Arabinose; D013401:Sugar Alcohol Dehydrogenases; D000449:Aldehyde Reductase; D050542:D-Xylulose Reductase; D019342:Acetic Acid; D014993:Xylitol