Phenetic studies on randomly amplified polymorphic DNA-polymerase chain reaction-variability of four geographical populations of Lutzomyia whitmani (Diptera: Psychodidae) in Brazil.
Previous evaluation of the genetic variability of four biogeographical populations of Lutzomyia whitmani from known foci of cutaneous leishmaniasis in Brazil demonstrated two main spatial clusters: Corte de Pedra-BA, Ilhéus-BA and Serra de Baturité-CE in the first cluster, and Martinho Campos-MG in the second. Further analysis showed a high degree of homogeneity in Corte de Pedra population but not in the others, which presented a significant percentage of specimens displaced from their phenon of origin (discrepant individuals). In the present work we analyzed the frequencies of association coefficients in the matrixes of similarity per population of Lutzomyia whitmani from both sexes and the general phenograms obtained, in a more detailed study of those discrepant specimens. Populational stability was observed for Corte de Pedra population, whereas the three remaining populations showed varying degrees of heterogeneity and different displacements according to sex. Our results strongly suggested the existence of a genetic flow between the lineages North-South/North-East and Ilhéus/Serra do Baturité of Lutzomyia whitmani.
de Souza
Carina Margonari
CM
Laboratório de Leishmanioses, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, Brazil.
Fortes-Dias
Consuelo Latorre
CL
Linardi
Pedro Marcos
PM
Dias
Edelberto Santos
ES
D000818:Animals; D001938:Brazil; D004247:DNA / Q000032:analysis; D005260:Female; D014644:Genetic Variation / Q000235:genetics*; D008297:Male; D010641:Phenotype; D010802:Phylogeny; D011576:Psychodidae / Q000145:classification / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique
D004247:DNA