The fibroblast-populated collagen matrix as a model of wound healing: a review of the evidence.
The fibroblast-populated collagen matrix (FPCM) has been utilized as an in vitro model of wound healing for more than 2 decades. It offers a reasonable approximation of the healing wound during the phases of established granulation tissue and early scar. The gross and microscopic morphology of the FPCM and the healing wound are similar at analogous phases. The processes of proliferation, survival/apoptosis, protein synthesis, and contraction act in similar directions in these two models, and the response to exogenous agents also is consistent between them. If its limitations are respected, then the FPCM can be used as a model of the healing wound.
Carlson
Mark A
MA
Department of Surgery, University of Nebraska Medical Center and the Omaha VA Medical Center, Omaha, Nebraska 68105, USA. macarlso@unmc.edu
Longaker
Michael T
MT
D000818:Animals; D018929:Cell Culture Techniques; D003094:Collagen / Q000502:physiology*; D016207:Cytokines / Q000502:physiology; D005347:Fibroblasts / Q000502:physiology*; D006097:Granulation Tissue / Q000503:physiopathology*; D008954:Models, Biological; D015203:Reproducibility of Results; D014945:Wound Healing / Q000502:physiology*
D016207:Cytokines; D003094:Collagen