title: Activation of mouse RAG-2 promoter by Myc-associated zinc finger protein.
abstract: Recombination activating gene-1 (RAG-1) and RAG-2 are expressed specifically in lymphocytes undergoing the antigen receptor gene rearrangement during the lymphocyte development. Our previous study showed that the -41 to -17 nucleotides (nt) 5' -upstream region of mouse RAG-2 were pre-requisite for the core promoter activity and that Pax-5/c-Myb/LEF-1 protein-protein complex was responsible for its activity in immature B cells. In this study, we show that the -65/-42 sequence, the non-conserved sequence between human and mouse RAG-2 promoter, is necessary for the full promoter activity for mouse RAG-2. Electrophoresis mobility shift assay revealed that Myc-associated zinc finger protein (MAZ) as well as SP1/3 binds a GA box in this region. Using chromatin immunoprecipitation, we show that MAZ binds the RAG-2 promoter region in pre-B cells. Furthermore, we show that MAZ synergistically activates the murine RAG-2 promoter with Pax-5/c-Myb/LEF-1 complex. These results first demonstrate that MAZ participates in activation of mouse RAG-2 promoter.
authors.affiliation: Department of Immunology, Faculty of Medicine, Toyama Medical and Pharmaceutical University, 2630, Sugitani, Toyama 930-0194, Japan.
authors.forename: Chun-Xiao
authors.initials: CX
authors.lastname: Wu
authors.forename: Wen-Pu
authors.initials: WP
authors.lastname: Zhao
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kishi
authors.forename: Junichi
authors.initials: J
authors.lastname: Dokan
authors.forename: Zhe-Xiong
authors.initials: ZX
authors.lastname: Jin
authors.forename: Xing-Cheng
authors.initials: XC
authors.lastname: Wei
authors.forename: Kazunari K
authors.initials: KK
authors.lastname: Yokoyama
authors.forename: Atsushi
authors.initials: A
authors.lastname: Muraguchi
mesh_terms: D000818:Animals; D016175:B-Lymphocyte Subsets / Q000166:cytology / Q000378:metabolism; D001482:Base Composition; D001483:Base Sequence; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D008156:Luciferases / Q000235:genetics; D051379:Mice; D041681:NIH 3T3 Cells; D009687:Nuclear Proteins; D011401:Promoter Regions, Genetic / Q000235:genetics; D020598:Proto-Oncogene Proteins c-myb / Q000737:chemistry / Q000502:physiology*; D016415:Sequence Alignment; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection; D016335:Zinc Fingers / Q000235:genetics / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D009687:Nuclear Proteins; D020598:Proto-Oncogene Proteins c-myb; C499311:RAG2 protein, human; C499308:Rag2 protein, mouse; D014157:Transcription Factors; C064655:V(D)J recombination activating protein 2; C064658:RAG-1 protein; D008156:Luciferases