Expression and activity of matrix metalloproteinases-2 and -9 in serum, core needle biopsies and tissue specimens of prostate cancer patients.
Prostate cancer is the most common cancer in men and second in the cancer-related frequency of mortality. Matrix metalloproteinases (MMPs) are involved in tumor invasion and metastasis in various malignancies. MMP-2 and MMP-9 are capable of digesting collagen type IV. Numerous studies have demonstrated an association between increased MMP-2 and -9 expression and tumor progression in various tumors. In this study, the expression and activities of MMP-2 and -9 were assessed in serum probes and tumor tissue from core needle biopsies and radical prostatectomies of 97 patients. MMP-2 and -9 serum expression was analyzed in a subgroup of 31 patients. MMP-9 serum expression was significantly increased in tumor patients and correlated with tumor grade. In contrast, the MMP-9 tissue expression and activity revealed no significant correlations to tumor stage or grade. The MMP-2 activity, however, showed a positive correlation for MMP-2 with tumor stage. Increased activity was predominantly detected in advanced tumor stages. Immunohistochemical analysis of MMP-2 expression demonstrated a positive association with tumor grade in prostatectomy specimens. The relative expression rates in biopsies matched in 65% with those of the prostatectomies. Detection of MMP-2 in core needle biopsies seems not to be a helpful marker for diagnostic purposes.
Sauer
Christian G
CG
University Hospital Mannheim, Department of Pathology, Mannheim, Germany.
Kappeler
Alexandra
A
Späth
Monika
M
Kaden
Jens J
JJ
Michel
Maurice S
MS
Mayer
Doris
D
Bleyl
Uwe
U
Grobholz
Rainer
R
D000230:Adenocarcinoma / Q000097:blood* / Q000201:enzymology* / Q000556:secondary; D000328:Adult; D000368:Aged; D001707:Biopsy, Needle; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000097:blood*; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D008875:Middle Aged; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000097:blood* / Q000201:enzymology* / Q000473:pathology
D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9