Localized chemical stimulation to micropatterned cells using multiple laminar fluid flows.
Micropatterns of the electrically conjugating cardiomyocytes were prepared on a single cell level by microcontact printing (microCP), and the localized chemical stimulations were applied to the cellular pattern using multiple laminar flows. The locally delivered 1-octanol inactivated part of the myocyte patterns, while the other areas retained the activity showing spontaneous and synchronous pulsatility. Since both the cells and flows were well defined as micropatterns and each integrated on a chip, the obtained results simply demonstrate the cellular responses in a single-cell network.
Kaji
Hirokazu
H
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai 980-8579, Japan.
Nishizawa
Matsuhiko
M
Matsue
Tomokazu
T
D020003:1-Octanol / Q000494:pharmacology; D000818:Animals; D001681:Biological Assay / Q000379:methods; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002642:Chick Embryo; D003600:Cytosol / Q000378:metabolism; D005353:Fibronectins / Q000737:chemistry; D017629:Gap Junctions / Q000187:drug effects; D006801:Humans; D044085:Microfluidics / Q000379:methods*; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry
D005353:Fibronectins; D012710:Serum Albumin, Bovine; D020003:1-Octanol; D002118:Calcium