Purification of a fibrinolytic enzyme (myulchikinase) from pickled anchovy and its cytotoxicity to the tumor cell lines.
A fibrinolytic enzyme, myulchikinase, from a Korean seasoning ingredient, myul-chi-jeot-gal, has been purified to electrophoretic homogeneity. The molecular mass of the myulchikinase was estimated to about 28 kDa by SDS-PAGE and gel filtration. Amino acid sequence of the NH2-terminal of myulchikinase showed significant homology with other fibrinolytic enzymes including trypsin from starfish, katsuwokinase, and rat pancreatic elastase II. The purified myulchikinase hydrolyzed various synthetic substrates with different substrate specificity and cytotoxic to the tumor cell lines.
Jeong
Yong-Kee
YK
Department of Life Science and Biotechnology, Dong-Eu University, Busan 614-714, Korea. ykjeong@dongeui.ac.kr
Yang
Woong Suk
WS
Kim
Kwang Hyuk
KH
Chung
Kyung Tae
KT
Joo
Woo Hong
WH
Kim
Jae Hyun
JH
Kim
Dong-Eun
DE
Park
Jeong Uck
JU
D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001940:Breast / Q000187:drug effects / Q000473:pathology; D045744:Cell Line, Tumor / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D010450:Endopeptidases / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004789:Enzyme Activation; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005399:Fishes / Q000378:metabolism*; D005404:Fixatives / Q000737:chemistry; D006801:Humans; D020014:K562 Cells; D008223:Lymphoma / Q000473:pathology; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009369:Neoplasms / Q000473:pathology*; D017386:Sequence Homology, Amino Acid
D000970:Antineoplastic Agents; D005343:Fibrinolytic Agents; D005404:Fixatives; D010450:Endopeptidases; C492920:myulchikinase