Intravitreous leptin concentrations in patients with proliferative diabetic retinopathy.
BACKGROUND
Previous studies have demonstrated that leptin is an angiogenic factor, and an increase in intravitreous leptin concentrations in diabetic patients with proliferative diabetic retinopathy (PDR) has also been described. The aim of the present study was to investigate the source of intravitreal leptin and to determine whether it is related to PDR activity.


METHODS
Serum and vitreous fluid samples were obtained simultaneously at the time of vitreoretinal surgery from 25 patients with PDR and 32 nondiabetic patients with nonproliferative ocular diseases (controls). Both groups were matched by age, sex, and body mass index. Leptin levels were determined by ELISA.


RESULTS
We did not observe any significant differences in vitreal levels of leptin between diabetic patients with PDR and controls (4.22 [2.6-9.7] versus 3.49 [1.9-9.7] ng/mL; P = not significant). Leptin concentrations were lower in vitreous fluid than in serum samples from diabetic patients with PDR (P < 0.001) and controls (P < 0.001). A direct correlation between serum and vitreous leptin concentrations was detected in diabetic patients with PDR (r = 0.60; P = 0.01) and controls (r = 0.51; P = 0.01). Finally, we did not observe any relationship between intravitreous leptin levels and PDR activity.


CONCLUSIONS
The intraocular production of leptin is not critically involved in the etiopathogenesis of PDR. In addition, our results suggest that serum diffusion is a relevant source of leptin in vitreous fluid.
Hernández
Cristina
C
Diabetes Research Unit, Hospital General Universitari Vall d'Hebron, Pg. Vall d'Hebron 119-129, 08035 Barcelona, Spain.
Lecube
Albert
A
Castellanos
Josep María
JM
Segura
Rosa María
RM
Garat
Magela
M
García-Arumí
José
J
Simó
Rafael
R
D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism; D003930:Diabetic Retinopathy / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D020738:Leptin / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D014822:Vitreous Body / Q000378:metabolism*
D020738:Leptin