title: Microfluidic valve with cored glass microneedle for microinjection.
abstract: In this paper, a new microinjection device was constructed by fusing a glass microneedle and a PDMS-based microvalve. The microneedle was fabricated via traditional micropipette pulling. The PDMS-based microvalve regulates the fluid flow in the microchannel and microneedle. The 'ON/OFF' operation of the valve was controlled by manually supplied pneumatic pressure. The valve membrane utilized a two level geometry to improve control at low flow rates. The relation between pressure and flow was measured and the results showed that very small volumes of fluid (>1 nl) could be controlled. The valve operation was investigated by monitoring the tip of the needle and pneumatic pressure simultaneously and it demonstrated very stable 'ON/OFF' operation to the pressure change.
authors.affiliation: Department of Biomedical Engineering, RM423, Anseo-dong, College of Medicine, Dankook University, Cheonan, Chungnam 330-714, Korea.
authors.forename: Sanghoon
authors.initials: S
authors.lastname: Lee
authors.forename: Wonje
authors.initials: W
authors.lastname: Jeong
authors.forename: David J
authors.initials: DJ
authors.lastname: Beebe
mesh_terms: D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004867:Equipment Design; D005898:Glass / Q000737:chemistry*; D008567:Membranes, Artificial; D044085:Microfluidics / Q000295:instrumentation*; D008845:Microinjections / Q000295:instrumentation*; D011312:Pressure
chemical_list: D004129:Dimethylpolysiloxanes; D008567:Membranes, Artificial