The mouse natural killer T cell-associated antigen recognized by U5A2-13 monoclonal antibody is intercellular adhesion molecule-1.
Natural Killer T (NKT) cells in mice are generally defined as NK1.1(+) T cells, although NK1.1 antigen is expressed only in C57BL/6 and related strains. This has precluded investigations of other strains. To find a novel NKT cell surface marker, we generated a monoclonal antibody (mAb), U5A2-13, which recognizes phenotypically and functionally similar populations to NKT cells in naïve mice irrespective of strain. Here, by using a COS-7 expressional cloning system, we molecularly cloned a cDNA encoding a protein reactive with the U5A2-13 mAb and then identified it as intercellular adhesion molecule-1 (ICAM-1). Importantly, the U5A2-13 mAb did not stain hepatic mononuclear cells from ICAM-1 gene disrupted mice. Furthermore, Pepscan method disclosed that the discontinuous epitope for U5A2-13 mAb is composed of three loops located in extracellular domain two of ICAM-1. Overall, U5A2-13, a mAb originally established for mouse NKT cells, recognizes a novel conformational epitope of ICAM-1.
Shimizu
Atsushi
A
Pharmacology Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-ku, Tokyo 104-0045, Japan.
Sasaki
Hiroki
H
Aoyagi
Kazuhiko
K
Yoshida
Mitsuzi
M
Kato
Kazunori
K
Heike
Yuji
Y
Ikarashi
Yoshinori
Y
Shirakawa
Kazuo
K
Takaue
Yoichi
Y
Miyajima
Atsushi
A
Terada
Masaaki
M
Nagai
Hideo
H
Wakasugi
Hiro
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003001:Cloning, Molecular; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005260:Female; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D018984:Epitopes, T-Lymphocyte; D018799:Intercellular Adhesion Molecule-1