PRDM5 is silenced in human cancers and has growth suppressive activities.
Several genes that contain the PR (PRDI-BF1 and RIZ) domain have been linked with human cancers. We describe here a new PR-domain-containing gene designated as PRDM5 (PFM2). A PRDM5 cDNA was isolated based on its homology to the PR domain of RIZ1 (PRDM2). The gene encodes an open reading frame of 630 amino acids and contains a PR domain in the NH-terminal region followed by 16 zinc finger motifs. Radiation hybrid analysis mapped PRDM5 to human chromosome 4q26, a region thought to harbor tumor suppressor genes for breast, ovarian, liver, lung, colon, and other cancers. The gene has a CpG island promoter and is silenced in human breast, ovarian, and liver cancers. A recombinant adenovirus expressing PRDM5 caused G2/M arrest and apoptosis upon infection of tumor cells. These results suggest that inactivation of PRDM5 may play a role in carcinogenesis.
Deng
Qingdong
Q
The Burnham Institute, 10901 N. Torrey Pines Road, La Jolla, CA 92037, USA.
Huang
Shi
S
D000595:Amino Acid Sequence; D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D001940:Breast; D001943:Breast Neoplasms; D006528:Carcinoma, Hepatocellular; D002453:Cell Cycle / Q000235:genetics; D002455:Cell Division / Q000235:genetics*; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000235:genetics*; D015226:Dinucleoside Phosphates / Q000737:chemistry; D005260:Female; D020868:Gene Silencing; D006801:Humans; D008099:Liver; D008113:Liver Neoplasms; D008969:Molecular Sequence Data; D010051:Ovarian Neoplasms; D014157:Transcription Factors
D004268:DNA-Binding Proteins; D015226:Dinucleoside Phosphates; D014157:Transcription Factors; C487762:PRDM5 protein, human; C015772:CpG dinucleotide