Cytogenetic analysis using fluorescence in situ hybridization (FISH) to evaluate occupational exposure to carcinogens.
Chromosomal aberrations determined by conventional method or fluorescence in situ hybridization (FISH) technique with whole chromosome painting are used as biomarkers of effect. Groups occupationally exposed to 1,3-butadiene (BD), acrylonitrile, ethyl benzene and benzene in petrochemical industry, and carcinogenic polycyclic aromatic hydrocarbons (c-PAHs) from ambient air were followed by conventional method and FISH painting for chromosomes # 1 and # 4, in total 383 subjects, including controls. No effect was observed by either method with exposure to 1,3-butadiene < 1mg/m(3) and acrylonitrile < 0.3mg/m(3). Ethyl benzene and benzene exposure significantly increased chromosomal aberrations by both methods, which decreased after the implementation of preventive measures. The genomic frequency of translocations by FISH calculated as FG/100 was significantly increased in city policemen versus control group exposed to c-PAHs from ambient air (1.72+/-1.57 versus 1.25+/-1.11, P<0.05). The method of FISH with whole chromosome painting seems to be more sensitive to detect chromosomal injury by occupational exposure to carcinogens than conventional method.
Sram
Radim J
RJ
Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine AS CR and Health Institute of Central Bohemia, 142 20 Prague 4, Czech Republic. sram@biomed.cas.cz
Beskid
Olena
O
Binkova
Blanka
B
Rossner
Pavel
P
Smerhovsky
Zdenek
Z
D000181:Acrylonitrile / Q000032:analysis / Q000633:toxicity; D000328:Adult; D001555:Benzene Derivatives / Q000032:analysis / Q000633:toxicity; D002070:Butadienes / Q000032:analysis / Q000633:toxicity; D002273:Carcinogens / Q000032:analysis*; D002617:Chemical Industry; D002869:Chromosome Aberrations / Q000139:chemically induced; D020223:Chromosome Painting; D020732:Cytogenetic Analysis; D005260:Female; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects*; D016495:Police; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity
D001555:Benzene Derivatives; D002070:Butadienes; D002273:Carcinogens; D005819:Genetic Markers; D011084:Polycyclic Aromatic Hydrocarbons; C031763:1,3-butadiene; C004912:ethylbenzene; D000181:Acrylonitrile