title: Excitatory action of prostanoids on the ferret isolated vagus nerve preparation.
abstract: We have investigated the actions of various prostanoid receptor agonists on an isolated preparation of the ferret cervical vagus using a grease-gap extracellular recording technique. The potency ranking for depolarization was BW245C (5-(6-carboxyhexyl)-1-(3-cyclohexyl-3-hydroxypropyl) hydantoin; DP-selective, EC50=0.14 microM)>prostaglandin E2 (nonselective EP agonist)>U-46619 (11alpha, 9alpha-epoxymethano-15S-hydroxyprosta-5Z,13E-dienoic acid; TP agonist)>prostaglandin F2alpha (FP receptor agonist). Sulprostone (EP1/EP3-selective), fluprostenol (FP-selective) and cicaprost and iloprost (both IP-selective) had minimal effects. It is likely that DP, EP2/EP4 and TP receptors are present on the vagal fibres of the ferret.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR, China.
authors.forename: Kelvin K W
authors.initials: KK
authors.lastname: Kan
authors.forename: Robert L
authors.initials: RL
authors.lastname: Jones
authors.forename: Man-Pui
authors.initials: MP
authors.lastname: Ngan
authors.forename: John A
authors.initials: JA
authors.lastname: Rudd
mesh_terms: D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid / Q000494:pharmacology; D000818:Animals; D001645:Biguanides / Q000494:pharmacology; D015237:Dinoprost / Q000494:pharmacology; D015232:Dinoprostone / Q000031:analogs & derivatives* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology; D011464:Epoprostenol / Q000031:analogs & derivatives* / Q000494:pharmacology; D005289:Ferrets; D006827:Hydantoins / Q000494:pharmacology; D016285:Iloprost / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D011453:Prostaglandins / Q000494:pharmacology*; D011461:Prostaglandins F, Synthetic / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology; D014630:Vagus Nerve / Q000187:drug effects* / Q000502:physiology
chemical_list: D001645:Biguanides; D006827:Hydantoins; D011453:Prostaglandins; D011461:Prostaglandins F, Synthetic; D012701:Serotonin; C006326:fluprostenol; C016767:sulprostone; C030669:BW 245C; D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; C065734:1-(3-chlorophenyl)biguanide; D015237:Dinoprost; D011464:Epoprostenol; D016285:Iloprost; D015232:Dinoprostone; C043867:cicaprost