Chemiluminescence quenching of pteroic acid-N-sulfonyl-acridinium-9-carboxamide conjugates by folate binding protein.
N(10)-Trifluoroacetylpteroic acid was conjugated to chemiluminescent N-sulfonylacridinium-9-carboxamide labels at the N(10) or 9-position carboxamide. Upon binding to folate binding protein the light output of the N(10) derivative (9) was quenched up to 62% upon triggering with basic peroxide, while the 9-position carboxamide conjugate (7) was quenched only 12%. The utility of this effect was demonstrated in a model homogeneous chemiluminescent assay for folic acid.
Adamczyk
Maciej
M
Department of Chemistry (D09MD), Abbott Laboratories, 100 Abbott Park Road, AP20, Abbott Park, IL 60064-6016, USA. maciej.adamczyk@abbott.com
Fino
James R
JR
Mattingly
Phillip G
PG
Moore
Jeffrey A
JA
Pan
You
Y
D002352:Carrier Proteins / Q000737:chemistry*; D058973:Folate Receptors, GPI-Anchored; D049449:Luminescence; D011956:Receptors, Cell Surface / Q000737:chemistry*
D002352:Carrier Proteins; D058973:Folate Receptors, GPI-Anchored; D011956:Receptors, Cell Surface