title: [Study on the subcloning, expression and immunoprotection with Schistosoma japonicum CAI gene].
abstract: OBJECTIVE
  To subclone and express the new gene of Schistosoma japonicum (Sj) CAI and evaluate the immunoprotective effect of the recombinant molecule.
  
  
  METHODS
  The cDNA of SjCAI gene was subcloned into expression vector pGEX-5X-3 to form recombinants which were then used to transform to E. coli strain ER 2566. Expression was induced by IPTG. The mice were vaccinated with the expressed protein and the immunoprotective effect was tested.
  
  
  RESULTS
  Fusion protein of SjGST-CAI was highly expressed in E. coli as inclusion bodies. The worm reduction rate and the liver egg reduction rate in vaccination group of SjGST-CAI were 29.87% and 63.71%, respectively.
  
  
  CONCLUSIONS
  SjCAI gene can be highly expressed in E. coli after subcloning into pGEX-5X-3 vector and the expressed fusion protein can induce immunoprotective effect against Sj in mice.
authors.affiliation: Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078.
authors.forename: Xiu-ju
authors.initials: XJ
authors.lastname: Luo
authors.forename: Shi-shan
authors.initials: SS
authors.lastname: Yuan
authors.forename: Xin-yuan
authors.initials: XY
authors.lastname: Yi
authors.forename: Xian-fang
authors.initials: XF
authors.lastname: Zeng
authors.forename: Shun-ke
authors.initials: SK
authors.lastname: Zhang
authors.forename: Lian-fei
authors.initials: LF
authors.lastname: Tang
authors.forename: Chun
authors.initials: C
authors.lastname: Cai
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Larry
authors.initials: L
authors.lastname: McReynolds
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D015870:Gene Expression; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology; D012549:Schistosoma japonicum / Q000235:genetics* / Q000276:immunology
chemical_list: D015801:Helminth Proteins; D011993:Recombinant Fusion Proteins