A novel Streptococcus organism identified in a case of fulminant endocarditis using 16S rDNA sequencing.
We report the identification of a virulent Streptococcus organism associated with fulminant endocarditis, using 16S rRNA gene amplification, sequencing and assembly from formalin-fixed, paraffin-embedded archival heart valve tissue, years after the autopsy of a patient.
Jobbagy
Zsolt
Z
Department of Pathology, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire, USA. zsolt_jobbagy@hitchcock.org
Fabian
Claire B
CB
Memoli
Vincent A
VA
Schwartzman
Joseph D
JD
D001018:Aortic Diseases / Q000209:etiology; D001344:Autopsy; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000032:analysis*; D004697:Endocarditis, Bacterial / Q000175:diagnosis* / Q000382:microbiology; D005260:Female; D005784:Gene Amplification; D006351:Heart Valves; D006801:Humans; D008875:Middle Aged; D016612:Paraffin Embedding; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013290:Streptococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013291:Streptococcus / Q000235:genetics*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S