title: A tale of two integrations, transgene and T-DNA: gene targeting by homologous recombination in rice.
abstract: The first successful and reproducible gene targeting by homologous recombination, without the concomitant occurrence of ectopic events, has been reported. This will be a powerful approach for the characterization of gene function in rice, an important crop and a model for other cereal species. Models have been proposed to explain gene replacement by homologous recombination, including a possible model for Agrobacterium-mediated gene targeting using a strong positive-negative selection.
authors.affiliation: National Institute for Basic Biology, Okazaki 444-8585, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Iida
authors.forename: Rie
authors.initials: R
authors.lastname: Terada
mesh_terms: D004269:DNA, Bacterial; D018390:Gene Targeting; D012275:Oryza / Q000235:genetics*; D011995:Recombination, Genetic; D019076:Transgenes
chemical_list: D004269:DNA, Bacterial; C036483:T-DNA