Rapid test for early detection of mycoplasma contamination of the continuous cell line J774.2.
The authors describe a rapid, simple and inexpensive method for the routine testing of mycoplasma contamination of the continuous mouse macrophage-like cell line J774.2 using specific anti-mouse monoclonal antibodies (antiCD14, antiCD80) and flow cytometry.
Loudová
Martina
M
Department of Clinical Immunology and Allergology, Faculty of Medicine in Hradec Kralove, University Hospital, Charles University in Prague, Sokolska tr. 408, 500 05 Hradec Kralove, Czech Republic. mloudova@post.cz
Novosad
Jakub
J
D000818:Animals; D018122:B7-1 Antigen / Q000032:analysis / Q000276:immunology; D001681:Biological Assay; D002460:Cell Line / Q000382:microbiology*; D005434:Flow Cytometry; D018950:Lipopolysaccharide Receptors / Q000032:analysis / Q000276:immunology; D008264:Macrophages / Q000276:immunology / Q000382:microbiology; D051379:Mice; D009174:Mycoplasma / Q000276:immunology / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction
D018122:B7-1 Antigen; D018950:Lipopolysaccharide Receptors