[Effect of testosterone on expression of androgen receptor in human monocytic cell line THP-1].
OBJECTIVE
To investigate the effect of testosterone on the expression of androgen receptor (AR) in human monocytic cell line THP-1.


METHODS
Cultured THP-1 cells were stimulated by testosterone with different concentrations and the AR protein was examined by Western blotting.


RESULTS
AR expression was observed in unstimulated THP-1 cells and the quantity of AR expression was reduced by testosterone treatment in a dose-dependent manner. A selective AR blocker completely abolished this inhibitory effect of testosterone on AR expresison.


CONCLUSIONS
Testosterone simulation time- and dose dependently reduces AR expression in THP-1 cells.
Liu
Chang-qing
CQ
Department of Cardiology, Nanfang Hostipal, First Military Medical University, Guangzhou 510515, China. liucqing@fimmu.com
Wu
Sai-zhu
SZ
Wang
Zi-dong
ZD
Lai
Wen-yang
WY
Sun
Fei
F
D015153:Blotting, Western; D002460:Cell Line; D006801:Humans; D009000:Monocytes / Q000737:chemistry / Q000187:drug effects*; D011944:Receptors, Androgen / Q000032:analysis*; D013739:Testosterone / Q000494:pharmacology*
D011944:Receptors, Androgen; D013739:Testosterone