Escherichia coli producing CTX-M-2 beta-lactamase in cattle, Japan.
From November 2000 to June 2001, Escherichia coli strains producing CTX-M-2 beta-lactamase were isolated from 6 (1.5%) of 396 cattle fecal samples and 2 (0.7%) of 270 surface swabs of cattle carcasses in Japan. The blaCTX-M-2 gene responsible for CTX-M-2 production was encoded on transferable plasmids, and the gene was transferred to E. coli CSH2 with a very high frequency (2 x 10(-4) to 6 x 10(-1) per donor cells) by conjugation. Random amplified polymorphic DNA analysis of nine isolates showed at least five different patterns. These findings suggest that CTX-M-2 producers might have originated from cattle through the use of cephalosporins such as ceftiofur and that cattle could be a reservoir of CTX-M-2-producing E. coli. Continuous and strategic surveillance of antimicrobial-resistant bacteria in livestock is essential to suppress further dissemination of these bacteria into society at large.
Shiraki
Yutaka
Y
Gifu Prefectural Office of Meat Inspection, Gifu, Japan. shiraki-yutaka@pref.gifu.lg.jp
Shibata
Naohiro
N
Doi
Yohei
Y
Arakawa
Yoshichika
Y
D000818:Animals; D002417:Cattle; D002511:Cephalosporins / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology*; D005243:Feces / Q000382:microbiology; D007564:Japan; D008826:Microbial Sensitivity Tests; D019105:Random Amplified Polymorphic DNA Technique; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification
D002511:Cephalosporins; C071167:beta-lactamase CTX-2; D001618:beta-Lactamases