title: [Synthesis of recombinant proteins from the American and European viruses of the porcine reproductive and respiratory syndrome in baculovirus expression system and their antigenic properties].
abstract: The baculovirus expression system was made use of to derive the recombinant nucleocapsid (N) proteins of the American and European virus types and of the porcine reproductive and respiratory syndrome (PRRS). The obtained products were purified by metal-affine chromatography and their specificity was confirmed in immunechemical reactions with reference monoclonal antibodies. The antigenic activity of recombinant proteins was studied by indirect immune enzyme assay (IEA) with porcine serum, which had been in advance characterized by the "HerdCheck" kit (IDEXX Co.). It was shown as possible to apply the derived recombinant antigens in determining, by indirect IEA, antibodies to the PRRS virus.
authors.forename: T V
authors.initials: TV
authors.lastname: Grebennikova
authors.forename: K P
authors.initials: KP
authors.lastname: Alekseev
authors.forename: R A
authors.initials: RA
authors.lastname: Gibadulin
authors.forename: M I
authors.initials: MI
authors.lastname: Musienko
authors.forename: V V
authors.initials: VV
authors.lastname: Tsybezov
authors.forename: V S
authors.initials: VS
authors.lastname: Bogdanova
authors.forename: A D
authors.initials: AD
authors.lastname: Zaberezhnyĭ
authors.forename: T I
authors.initials: TI
authors.lastname: Aliper
authors.forename: E A
authors.initials: EA
authors.lastname: Nepoklonov
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000096:biosynthesis / Q000276:immunology*; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D005822:Genetic Vectors; D019590:Nucleocapsid Proteins / Q000096:biosynthesis / Q000276:immunology*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000097:blood; D019316:Porcine respiratory and reproductive syndrome virus / Q000737:chemistry / Q000276:immunology*; D011994:Recombinant Proteins / Q000096:biosynthesis; D013045:Species Specificity; D013552:Swine
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins