title: Bacterial phospholipid molecular species analysis by ion-pair reversed-phase HPLC/ESI/MS.
abstract: This work set out to optimize the detection and separation of several phospholipid molecular species on a reversed-phase column with the use of an electrospray ionization/mass spectrometry-compatible counter-ion. An application of this technique concerned a qualitative and quantitative analysis of bacterial membrane phospholipids extracted from Corynebacterium species strain 8. The phospholipid classes of strain 8 were identified as phosphatidylglycerol, phosphatidylinositol, diphosphatidylglycerol, and a peculiar lipid compound, acyl phosphatidylglycerol. Most of the molecular species structures were elucidated, and regarding phosphatidylglycerol, the fatty acid positions were clearly determined with the calculation of the sn-2/sn-1 intensity ratio of the fatty acyl chain fragments.
authors.affiliation: Laboratoire de Chimie Analytique de l'Environnement, Unité Mixte de Recherche 6171, Institut Fédératif de Recherche Pôle Méditerannéen des Sciences de l'Environement 112, Europôle de l'Arbois, BP 80, 13545 Aix-en-Provence Cedex 4, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Mazzella
authors.forename: Josiane
authors.initials: J
authors.lastname: Molinet
authors.forename: Agung Dhamar
authors.initials: AD
authors.lastname: Syakti
authors.forename: Alain
authors.initials: A
authors.lastname: Dodi
authors.forename: Pierre
authors.initials: P
authors.lastname: Doumenq
authors.forename: Jacques
authors.initials: J
authors.lastname: Artaud
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Bertrand
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003352:Corynebacterium / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis; D008722:Methods; D010743:Phospholipids / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013237:Stereoisomerism
chemical_list: D005227:Fatty Acids; D010743:Phospholipids