Microbarcode sorting device.
A novel and simple microfluidic device was developed for sorting 20 microm thick glass microbarcodes for imaging or scanning at the completion of a bead-based assay. Specifically, the microbarcodes are dried and kept from stacking on top of one another such that a monolayer of microbarcodes is created and the microbarcodes lay flat on a surface. The microbarcode sorting device consists of a reservoir, a sorting region, and a network of microchannels. With minimal microbarcodes loss, a monolayer of microbarcodes is created and trapped inside the sorting region for conveniently imaging or scanning. The device can also be used for any geometrical shaped beads with a range of thicknesses and can be adapted to a 96-well plate format for high throughput analysis.
Yuen
Po Ki
PK
Science and Technology, Corning Incorporated, Corning, New York 14831-0001, USA. yuenp@corning.com
Despa
Mircea
M
Li
Cheng-Chung Jim
CC
Dejneka
Matthew J
MJ
D000818:Animals; D001710:Biotin / Q000737:chemistry; D002417:Cattle; D003625:Data Collection / Q000295:instrumentation*; D001330:Electronic Data Processing / Q000295:instrumentation* / Q000379:methods; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D044085:Microfluidics / Q000295:instrumentation*; D008856:Microscopy, Fluorescence; D008863:Microspheres; D011437:Propylamines; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012821:Silanes / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry
D007074:Immunoglobulin G; D011437:Propylamines; D012821:Silanes; D012710:Serum Albumin, Bovine; D001710:Biotin; D019809:Streptavidin; D016650:Fluorescein-5-isothiocyanate; C477625:amino-propyl-triethoxysilane