Down-regulation of apoptosis-inducing factor protein by RNA interference inhibits UVA-induced cell death.
Apoptosis-inducing factor (AIF) is a caspase-independent apoptosis effector. UVA-induced Raji cell death was not completely inhibited by pan-caspase inhibitor zVAD.fmk. Moreover, AIF translocated from its normal location, the mitochondrial intermembrane space, into the nucleus, and induced peripheral chromatin condensation during the early stage of UVA-inducing cell death. Enforced expression of AIF can induce Raji cell death in a caspase-independent manner. Down-regulation of AIF protein level by RNA interference (RNAi) can reduce UVA-induced Raji cell death, but the combination of down-regulation of AIF and zVAD.fmk almost completely inhibited UVA-induced Raji cell death. All these suggest that caspase and AIF are two independent pathways and that UVA-induced Raji cell death is dependent on caspase and AIF.
Yuan
Chang-Qing
CQ
Laboratory of Radiation Medicine, Institute of Tropical Medicine, The First Military Medical University, Guangzhou 510515, China. yuanchq@hotmail.com
Li
Ya-Na
YN
Zhang
Xu-Feng
XF
D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D017209:Apoptosis / Q000502:physiology* / Q000528:radiation effects; D051033:Apoptosis Inducing Factor; D001402:B-Lymphocytes / Q000166:cytology / Q000528:radiation effects; D001483:Base Sequence; D061945:Caspase Inhibitors; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D015536:Down-Regulation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005420:Flavoproteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D021381:Protein Transport; D034622:RNA Interference / Q000502:physiology*; D013698:Templates, Genetic; D014162:Transfection; D014466:Ultraviolet Rays / Q000009:adverse effects
C494829:AIFM1 protein, human; D000590:Amino Acid Chloromethyl Ketones; D051033:Apoptosis Inducing Factor; D061945:Caspase Inhibitors; D004791:Enzyme Inhibitors; D005420:Flavoproteins; D008565:Membrane Proteins; C096713:benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; D020169:Caspases