Exercise induces BDNF and synapsin I to specific hippocampal subfields.
To assess the relationship between brain-derived neurotrophic factor (BDNF) and synapsin I in the hippocampus during exercise, we employed a novel microsphere injection method to block the action of BDNF through its tyrosine kinase (Trk) receptor and subsequently measure the mRNA levels of synapsin I, using real-time TaqMan RT-PCR for RNA quantification. After establishing a causal link between BDNF and exercise-induced synapsin I mRNA levels, we studied the exercise-induced distribution of BDNF and synapsin I in the rodent hippocampus. Quantitative immunohistochemical analysis revealed increases of BDNF and synapsin I in CA3 stratum lucidum and dentate gyrus, and synapsin I alone in CA1 stratum radiatum and stratum laconosum moleculare. These results indicate that exercise induces plasticity of select hippocampal transsynaptic circuitry, possibly comprising a spatial restriction on synapsin I regulation by BDNF.
Vaynman
Shoshanna
S
Department of Physiological Science, UCLA, Los Angeles, California 90095, USA.
Ying
Zhe
Z
Gómez-Pinilla
Fernando
F
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D002227:Carbazoles / Q000008:administration & dosage; D004791:Enzyme Inhibitors / Q000008:administration & dosage; D006624:Hippocampus / Q000201:enzymology / Q000378:metabolism*; D026121:Indole Alkaloids; D008297:Male; D008863:Microspheres; D009473:Neuronal Plasticity / Q000502:physiology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020813:Receptor, trkB / Q000037:antagonists & inhibitors / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D016704:Synapsins / Q000378:metabolism*
D019208:Brain-Derived Neurotrophic Factor; D002227:Carbazoles; D004791:Enzyme Inhibitors; D026121:Indole Alkaloids; D016704:Synapsins; C049985:staurosporine aglycone; D020813:Receptor, trkB; D011493:Protein Kinase C