title: Assay of neonatal TSH from blood spots using the Architect i2000, an immunoassay analyzer.
abstract: OBJECTIVE
  To assess the Architect i2000 for measuring TSH from neonatal blood spots.
  
  
  METHODS
  Blood collected on filter paper was eluted and assayed on the Architect.
  
  
  RESULTS
  Recovery of TSH was close to 100%. Within-run and between-run precisions were 4.2-8.2% and 14.5-18.1%. Correlation with the Wallac Delphia method was excellent (r = 0.96). Linearity, sensitivity, and slope were 0-400 mU/l, 0.15 mU/l, and 0.86, respectively. The results obtained by this method on the Architect allowed us to differentiate normals from affected patients with the same reliability as the previous method.
  
  
  CONCLUSIONS
  Measurement of TSH on the Architect is an excellent method for detecting congenital hypothyroidism.
authors.affiliation: The Provincial Laboratory, Saskatchewan, Canada S4S 5W6.
authors.forename: Joyce
authors.initials: J
authors.lastname: LePage
authors.forename: Sherry
authors.initials: S
authors.lastname: Peters
authors.forename: Denis C
authors.initials: DC
authors.lastname: Lehotay
mesh_terms: D001800:Blood Specimen Collection; D003409:Congenital Hypothyroidism; D006801:Humans; D007037:Hypothyroidism / Q000175:diagnosis; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D007231:Infant, Newborn; D015997:Neonatal Screening; D015203:Reproducibility of Results; D012525:Saskatchewan; D013972:Thyrotropin / Q000097:blood*
chemical_list: D013972:Thyrotropin