title: Host and bacterial factors affecting induction of immune responses to flagellin expressed by attenuated Salmonella vaccine strains.
abstract: Previous observations demonstrated that the delivery of recombinant Salmonella enterica serovar Dublin strains to mice via mucosal routes did not efficiently activate systemic and secreted antibody responses to either type d flagellin or genetically fused heterologous B-cell epitopes, thus reducing the usefulness of the protein as a carrier of epitopes for vaccine purposes. In this work, we investigated murine systemic and mucosal flagellin immunogenicity after oral immunization with attenuated Salmonella strains. The reduced anti-type d flagellin antibody responses in mice immunized via mucosal routes with three doses of flagellated S. enterica serovar Dublin strains were not caused by oral tolerance and could not be restored by coadministration of a mucosal adjuvant. The induction of antibody responses to Salmonella flagellins was shown to differ according to the genetic background, but not the haplotype, of the mouse lineage. Moreover, BALB/c mice orally immunized with S. enterica serovar Typhimurium strains developed anti-type i flagellin sera and secreted antibody responses, which indicated that the serovar of the Salmonella vaccine strain also affected flagellin immunogenicity. Analyses of cytokine responses of BALB/c mice immunized with three oral doses of flagellated S. enterica serovar Dublin vaccine strains showed that, in spite of the lack of antibody responses, elevated type d flagellin-specific CD4-cell-activation-dependent gamma interferon (IFN-gamma) and interleukin-10 responses were elicited after the administration of the vaccine strains via either parenteral or mucosal routes. Similar cytokine production patterns were detected to a T-cell heterologous epitope, derived from the CFA/I fimbriae of enterotoxigenic Escherichia coli (ETEC), in mice orally immunized with a Salmonella vaccine strain expressing hybrid flagella. These results indicate that the immunogenicities of Salmonella flagellins can differ significantly, depending on the murine host and on the bacterial vector used, and demonstrate that the induction of CD4-cell-activation-dependent IFN-gamma production represents a major immune response triggered by flagellin and in-frame fused heterologous T-cell epitopes after the oral administration of recombinant S. enterica serovar Dublin vaccine strains.
authors.affiliation: Division of Technological Development and Production, Butantan Institute, São Paulo University, São Paulo, Brazil.
authors.forename: M E
authors.initials: ME
authors.lastname: Sbrogio-Almeida
authors.forename: T
authors.initials: T
authors.lastname: Mosca
authors.forename: L M
authors.initials: LM
authors.lastname: Massis
authors.forename: I A
authors.initials: IA
authors.lastname: Abrahamsohn
authors.forename: L C S
authors.initials: LC
authors.lastname: Ferreira
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000284:Administration, Oral; D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D005408:Flagellin / Q000276:immunology*; D007108:Immune Tolerance; D018928:Immunity, Mucosal; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D022562:Salmonella Vaccines / Q000008:administration & dosage / Q000276:immunology / Q000494:pharmacology*; D019779:Salmonella enterica / Q000276:immunology* / Q000472:pathogenicity; D012486:Salmonella typhimurium / Q000276:immunology / Q000472:pathogenicity; D014613:Vaccines, Attenuated / Q000276:immunology / Q000494:pharmacology
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D022562:Salmonella Vaccines; D014613:Vaccines, Attenuated; D005408:Flagellin