Ascorbic acid-2-o-phosphate-6-o-palmitate protecting the human umbilical cord vein endothelial cells against hydrogen peroxide and tert-butyl hydroperoxide induced cytotoxicity.
Reactive oxygen species are believed to play a role in the development of several diseases including vascular diseases and the aging process. It is reported that increased reactive oxygen species were implicated as an important mechanism that contributes to endothelial dysfunction. So, human umbilical cord vein endothelial cells were used to study the antioxidative effect of L-ascorbic acid and its derivative. The study indicated that L-ascorbic acid as a traditional antioxidant was instable and could protect the cells against hydrogen peroxide induced cytotoxicity as its concentration below 50 microg/ml, but hardly could protect the cells against tert-butyl hydroperoxide induced cytotoxicity. Ascorbic acid-2-o-phosphate-6-o-palmitate could effectively protect the cells against hydrogen peroxide and tert-butyl hydroperoxide induced cytotoxicity, and exhibited no cytotoxicity within the tested concentration range. The study indicated that ascorbic acid-2-o-phosphate-6-o-palmitate could not only significantly reduce the intracellular reactive oxygen species level in 3 h culture, but also increase the cell viability in 15 h culture. In addition, ascorbic acid-2-o-phosphate-6-o-palmitate could keep stable in RPMI-1640 medium and water for 4 days, permeate the cell membrane, which in turn may scavenge the intracellular reactive oxygen species, increase the cell viability and the plasminogen activators'activity. All the above results suggested that addition of some hydrophobic groups to the traditional antioxidants could form novel compounds with better properties.
Fan
Shifen
S
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, PR China.
Wei
Dongzhi
D
Liu
Jianwen
J
Xin
Xiujuan
X
Tong
Wangyu
W
Miwa
Nobuhiko
N
D000975:Antioxidants / Q000627:therapeutic use*; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000627:therapeutic use*; D002478:Cells, Cultured; D006801:Humans; D006861:Hydrogen Peroxide / Q000009:adverse effects / Q000037:antagonists & inhibitors*; D017382:Reactive Oxygen Species / Q000009:adverse effects*; D014471:Umbilical Veins / Q000187:drug effects; D020122:tert-Butylhydroperoxide / Q000009:adverse effects / Q000037:antagonists & inhibitors*
C403966:6-O-palmitol-ascorbate-2-O-phosphate; D000975:Antioxidants; D017382:Reactive Oxygen Species; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid