An evaporation-based microfluidic sample concentration method.
We present a method for sample concentration within microfluidic devices using evaporation-induced flow. Evaporation-induced flow is easy to incorporate into microfluidic designs and can be used to concentrate a wide variety of molecules. The practicality of this method was demonstrated with 0.2 microm fluorescent spheres and FITC-labeled BSA. Thirty two percent of the 0.6 microL fluorescent sphere suspension was concentrated into a well within a microfluidic device. In the same amount of time, 93% of the 0.6 microL FITC-labeled BSA solution was concentrated.
Walker
Glenn M
GM
Biomedical Engineering Department, University of Wisconsin-Madison, Madison, WI 53706, USA.
Beebe
David J
DJ