title: Expression of calretinin by fetal otocyst cells after transplantation into damaged rat utricle explants.
abstract: Severe damage by acoustic overstimulation or ototoxins induces inner ear hair cell loss, resulting in permanent hearing loss and balance disorders because hair cell regeneration scarcely occurs in the inner ear sensory organs of mammals. In this study, to evaluate the possibilities of cell transplantation therapy for damaged inner ear sensory organs, dissociated cell cultures of fetal otocyst cells (FOCs) were established from embryonic day 12.5 (E12.5) rat inner ears, and transplanted into gentamicin-treated explants of vestibular sensory epithelia. Two weeks after transplantation, immunohistochemical analysis demonstrated that some of the grafted FOCs survived within the vestibular sensory epithelia and expressed epitopes of calretinin. one of the hair cell marker proteins. These findings indicate that FOCs have the potential to migrate into damaged vestibular epithelia and differentiate into hair cell immunophenotypes. Cell transplantation therapy may be available for functional regeneration in inner ear diseases.
authors.affiliation: Department of Otolaryngology--Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors.forename: Tae-Soo
authors.initials: TS
authors.lastname: Kim
authors.forename: Ken
authors.initials: K
authors.lastname: Kojima
authors.forename: Akiko T
authors.initials: AT
authors.lastname: Nishida
authors.forename: Kei
authors.initials: K
authors.lastname: Tashiro
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Lee
authors.forename: Kiyohiro
authors.initials: K
authors.lastname: Fujino
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nakagawa
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Naito
authors.forename: Koichi
authors.initials: K
authors.lastname: Omori
authors.forename: Phillippe
authors.initials: P
authors.lastname: Lefebvre
authors.forename: Juichi
authors.initials: J
authors.lastname: Ito
mesh_terms: D000818:Animals; D064032:Calbindin 2; D018929:Cell Culture Techniques; D007758:Ear, Inner / Q000166:cytology / Q000196:embryology* / Q000473:pathology; D004848:Epithelium / Q000502:physiology; D006198:Hair Cells, Auditory / Q000166:cytology*; D007150:Immunohistochemistry; D016130:Immunophenotyping; D007381:Intermediate Filament Proteins / Q000378:metabolism; D007759:Labyrinth Diseases / Q000209:etiology / Q000628:therapy*; D008856:Microscopy, Fluorescence; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D051381:Rats; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D012444:Saccule and Utricle / Q000166:cytology / Q000473:pathology; D033581:Stem Cell Transplantation / Q000379:methods; D014945:Wound Healing / Q000502:physiology
chemical_list: C576512:Calb2 protein, rat; D064032:Calbindin 2; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D064030:S100 Calcium Binding Protein G