title: Effectiveness of EDTA and EDTA-T brushing on the removal of root surface smear layer.
abstract: The purpose of this study was to compare the removal of root surface smear layer following active application of EDTA gel and EDTA-T (texapon) gel in different concentrations (5%, 10%, 15%, 20% and 24%), using scanning electron microscopy. A total of 220 dentin blocks obtained from the root surfaces of extracted teeth were divided into 3 groups: Group I - (control) application of saline solution (n = 20); Group II - EDTA gel (pH 7.0) was applied in the following concentrations: 5%, 10%, 15%, 20% and 24% (n = 100); Group III - EDTA-T gel (pH 7.0) applied in the same concentrations described above (n = 100). The photomicrographs were evaluated by one calibrated examiner using a smear layer removal index and following statistical analysis (Kruskal-Wallis test). The results demonstrated that the specimens treated with EDTA and EDTA-T gel presented a better smear layer removal than the control group (p < 0.01); no statistically significant differences were observed between the EDTA and EDTA-T groups and between the concentrations tested (Mann-Whitney, p > 0.05). Within the limits of this study, it can be concluded that all treatment modalities effectively removed the smear layer from the root surface. The addition of texapon into the EDTA gel formulation did not increase its effectiveness.
authors.affiliation: Araraquara Dental School, São Paulo State University.
authors.forename: José Eduardo Cezar
authors.initials: JE
authors.lastname: Sampaio
authors.forename: Ricardo Samih Georges Abi
authors.initials: RS
authors.lastname: Rached
authors.forename: Gibson Luiz
authors.initials: GL
authors.lastname: Pilatti
authors.forename: Letícia Helena
authors.initials: LH
authors.lastname: Theodoro
authors.forename: Luiz Henrique Carvalho
authors.initials: LH
authors.lastname: Batista
mesh_terms: D001641:Bicuspid / Q000187:drug effects / Q000648:ultrastructure; D004492:Edetic Acid / Q000008:administration & dosage / Q000627:therapeutic use*; D005782:Gels; D006801:Humans; D008855:Microscopy, Electron, Scanning; D012494:Sampling Studies; D016369:Smear Layer; D012967:Sodium Dodecyl Sulfate / Q000008:administration & dosage / Q000627:therapeutic use; D018709:Statistics, Nonparametric; D013501:Surface-Active Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D013997:Time Factors; D014092:Tooth Root / Q000187:drug effects* / Q000648:ultrastructure; D014099:Toothbrushing
chemical_list: D005782:Gels; D013501:Surface-Active Agents; C075204:Texapon; D012967:Sodium Dodecyl Sulfate; D004492:Edetic Acid