title: [Schistosoma japonicum: screening of cDNA library with sera from self-cured water buffaloes (Bos buffelus)].
abstract: OBJECTIVE
  To understand and identify the molecules related to the self-cure of Schistosoma japonicum infection in water buffaloes.
  
  
  METHODS
  The S. japonicum adult worm cDNA library was immunologically screened with the sera of self-cured water buffaloes. The positive clones were identified, cloned, sequenced and analysed with software.
  
  
  RESULTS
  Three genes encoding antigens relevant to sera antibodies in water buffaloes were cloned and sequenced. These antigens included paramyosin (Sj97), GST, carbonyl reductase-like 20-beta-hydroxysteroid dehydrogenase(CR/20-beta-HSD).
  
  
  CONCLUSIONS
  Sera from self-cured S. japonicum infected water buffaloes can be used to screen adult worm cDNA library for vaccine development.
authors.affiliation: Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025.
authors.forename: Wen-qin
authors.initials: WQ
authors.lastname: Wang
authors.forename: Shu-xian
authors.initials: SX
authors.lastname: Liu
authors.forename: Guang-cheng
authors.initials: GC
authors.lastname: Song
authors.forename: Yu-xin
authors.initials: YX
authors.lastname: Xu
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Cao
authors.forename: Jia-xu
authors.initials: JX
authors.lastname: Chen
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000276:immunology*; D018076:DNA, Complementary / Q000276:immunology*; D018509:DNA, Helminth / Q000276:immunology*; D015723:Gene Library; D007106:Immune Sera / Q000276:immunology; D012549:Schistosoma japonicum / Q000235:genetics* / Q000276:immunology; D012554:Schistosomiasis japonica / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology
chemical_list: D018076:DNA, Complementary; D018509:DNA, Helminth; D007106:Immune Sera; D019444:Vaccines, DNA