Assays to measure stress-activated MAPK activity.
Mitogen-activated protein kinases (MAPKs) are activated by a wide variety of cellular stimuli and involved in the regulation of most, if not all, cellular processes. Among them, the c-Jun N-terminal kinase and p38 MAPKs are predominantly induced in response to proinflammatory cytokines and most environmental stresses, such as pathogenic insults, heat and osmotic shock, ultraviolet light, protein synthesis inhibitors, DNA-damaging agents, and withdrawal of growth factors; thus, they are also known as stress-activated protein kinases. In this chapter we describe alternative methods designed to measure the activity of these stress-activated protein kinases, c-Jun N-terminal kinase, and p38 MAPKs.
Caelles
Carme
C
Institut de Recerca Biomèdica de Barcelona-Parc Científic de Barcelona, Department of Biochemistry and Molecular Biology, University of Barcelona, Spain.
Morales
Mónica
M
D001681:Biological Assay / Q000379:methods*; D004249:DNA Damage; D004789:Enzyme Activation; D006367:HeLa Cells; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases; D020935:MAP Kinase Signaling System / Q000502:physiology; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases
D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases