title: Involvement of PKC and PKA in the inhibitory effect of leptin on intestinal galactose absorption.
abstract: Studies from our laboratory have demonstrated that leptin inhibits galactose absorption in vitro by acting on the Na(+)/glucose cotransporter SGLT1. Since PKC and PKA are involved in the regulation of SGLT1 and leptin is able to activate these kinases, we have investigated the possible implication of PKC and PKA in the inhibition of sugar absorption by leptin in rat small intestinal rings. Inhibition of 1 mM galactose uptake by 0.2 nM leptin is blocked by 2 microM chelerythrine, a PKC inhibitor, which by itself does not affect galactose uptake. However, 1 microM H-89, a PKA inhibitor, inhibits galactose uptake and does not block leptin inhibition. Biochemical assays show that the inhibitory effect of leptin is accompanied by a approximately 2-fold increase in PKA and PKC activity. These findings indicate that the activation of PKC is more relevant than PKA activation in the inhibition of galactose absorption by leptin.
authors.affiliation: Department of Physiology and Nutrition, University of Navarra, Pamplona 31008, Spain.
authors.forename: Jaione
authors.initials: J
authors.lastname: Barrenetxe
authors.forename: Neira
authors.initials: N
authors.lastname: Sainz
authors.forename: Ana
authors.initials: A
authors.lastname: Barber
authors.forename: M Pilar
authors.initials: MP
authors.lastname: Lostao
mesh_terms: D000818:Animals; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism*; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism*; D020738:Leptin / Q000502:physiology*; D008297:Male; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D020738:Leptin; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D005690:Galactose