title: Disruption of type 5 adenylyl cyclase negates the developmental increase in Galphaolf expression in the striatum.
abstract: The two stimulatory G protein alpha subunits, Galphas and Galphaolf, activate adenylyl cyclase in a similar way. We examined whether type 5 adenylyl cyclase knockout, the major striatal isoform, can differentially and/or developmentally change the expression of these G proteins in the striatum. Galphas and Galphaolf expressions at birth were unaffected in knockouts, which, however, demonstrated a blunted developmental increase in Galphaolf, but not Galphas. Adenylyl cyclase activity was unaffected at birth, but subsequently became lower in knockouts. These findings suggest that type 5 adenylyl cyclase does not contribute to striatal cAMP signaling at birth. However, it may play an important role in developmental changes in the expression of Galphaolf, but not Galphas.
authors.affiliation: Departments of Physiology and Medicine, Yokohama City University School of Medicine, Yokohama 234-0002, Japan.
authors.forename: Tamio
authors.initials: T
authors.lastname: Iwamoto
authors.forename: Kousaku
authors.initials: K
authors.lastname: Iwatsubo
authors.forename: Satoshi
authors.initials: S
authors.lastname: Okumura
authors.forename: Yoko
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: Takashi
authors.initials: T
authors.lastname: Tsunematsu
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Toya
authors.forename: Denis
authors.initials: D
authors.lastname: Hervé
authors.forename: Satoshi
authors.initials: S
authors.lastname: Umemura
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ishikawa
mesh_terms: D000262:Adenylyl Cyclases / Q000235:genetics / Q000502:physiology*; D000367:Age Factors; D000818:Animals; D003342:Corpus Striatum / Q000378:metabolism*; D044385:GTP-Binding Protein alpha Subunits / Q000096:biosynthesis; D018507:Gene Expression Regulation, Developmental; D020962:Heterotrimeric GTP-Binding Proteins / Q000096:biosynthesis*; D007527:Isoenzymes / Q000235:genetics / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D015398:Signal Transduction
chemical_list: D044385:GTP-Binding Protein alpha Subunits; D007527:Isoenzymes; C084618:olfactory G protein subunit alpha olf; D020962:Heterotrimeric GTP-Binding Proteins; D000262:Adenylyl Cyclases; C419510:adenylyl cyclase type V