title: Production of exopolysaccharides by Antarctic marine bacterial isolates.
abstract: OBJECTIVE
  This study was undertaken to examine and characterize Antarctic marine bacterial isolates and the exopolysaccharides (EPS) they produce in laboratory culture.
  
  
  RESULTS
  Two EPS-producing bacterial strains CAM025 and CAM036 were isolated from particulate material sampled from seawater and sea ice in the southern ocean. Analyses of 16S rDNA sequences placed these isolates in the genus Pseudoalteromonas. In batch culture, both strains produced EPS. The yield of EPS produced by CAM025 was 30-fold higher at -2 and 10 degrees C than at 20 degrees C. Crude chemical analyses showed that these EPS were composed primarily of neutral sugars and uronic acids with sulphates. Gas chromatographic analysis of monosaccharides confirmed these gross compositional findings and molar ratios of monosaccharides revealed differences between the two EPS.
  
  
  CONCLUSIONS
  The EPS produced by Antarctic bacterial isolates examined in this study appeared to be polyanionic and, therefore, 'sticky' with respect to cations such as trace metals.
  
  
  CONCLUSIONS
  As the availability of iron as a trace metal is of critical importance in the southern ocean where it is know to limit primary production, the role of these bacterial EPS in the Antarctic marine environment has important ecological implications.
authors.affiliation: School of Agricultural Science, University of Tasmania, Hobart, Tasmania, Australia. c.a.mancuso@utas.edu.au
authors.forename: C A
authors.initials: CA
authors.lastname: Mancuso Nichols
authors.forename: S
authors.initials: S
authors.lastname: Garon
authors.forename: J P
authors.initials: JP
authors.lastname: Bowman
authors.forename: G
authors.initials: G
authors.lastname: Raguénès
authors.forename: J
authors.initials: J
authors.lastname: Guézennec
mesh_terms: D000864:Antarctic Regions; D003124:Colorimetry / Q000379:methods; D007053:Ice; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009005:Monosaccharides / Q000032:analysis; D009792:Oceans and Seas; D010802:Phylogeny; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D031161:Pseudoalteromonas / Q000235:genetics / Q000378:metabolism*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D014871:Water Microbiology
chemical_list: D007053:Ice; D009005:Monosaccharides; D011135:Polysaccharides, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S