In situ fabricated porous filters for microsystems.
Porous filters were fabricated inside a microchannel using emulsion photo-polymerization. The porosity of the filter is dependent on the composition of the pre-polymer mixture. The morphology and flow resistance of four filters with varying water and cross-linker concentrations was studied. The porous filter was used to separate samples, such as whole blood to cell/serum components, based on size. The efficiency of cell/serum separation by the porous filter was tested by performing a glucose-6-phosphate dehydrogenase assay and was found to be comparable to separation by centrifuge. The porous filter thus mimics the functionality of the centrifuge, with the added benefits of no power requirements and ability to handle small sample volumes.
Moorthy
Jaisree
J
Department of Biomedical Engineering, University of Wisconsin at Madison, WI 53706, USA.
Beebe
David J
DJ
D000098:Acetophenones / Q000737:chemistry; D000818:Animals; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D005374:Filtration / Q000295:instrumentation* / Q000379:methods; D005954:Glucosephosphate Dehydrogenase / Q000097:blood; D006440:Hemofiltration / Q000295:instrumentation / Q000379:methods; D007700:Kinetics; D008689:Methacrylates / Q000737:chemistry; D018613:Microscopy, Confocal; D008904:Miniaturization / Q000295:instrumentation* / Q000379:methods; D010316:Particle Size; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D011312:Pressure; D011817:Rabbits
D000098:Acetophenones; D008689:Methacrylates; D011108:Polymers; C005044:hydroxyethyl methacrylate; C004919:ethylene dimethacrylate; D005954:Glucosephosphate Dehydrogenase