Innate immunity in a pyralid moth: functional evaluation of domains from a beta-1,3-glucan recognition protein.
Invertebrates, like vertebrates, utilize pattern recognition proteins for detection of microbes and subsequent activation of innate immune responses. We report structural and functional properties of two domains from a beta-1,3-glucan recognition protein present in the hemolymph of a pyralid moth, Plodia interpunctella. A recombinant protein corresponding to the first 181 amino-terminal residues bound to beta-1,3-glucan, lipopolysaccharide, and lipoteichoic acid, polysaccharides found on cell surfaces of microorganisms, and also activated the prophenoloxidase-activating system, an immune response pathway in insects. The amino-terminal domain consists primarily of an alpha-helical secondary structure with a minor beta-structure. This domain was thermally stable and resisted proteolytic degradation. The 290 residue carboxyl-terminal domain, which is similar in sequence to glucanases, had less affinity for the polysaccharides, did not activate the prophenoloxidase cascade, had a more complicated CD spectrum, and was heat-labile and susceptible to proteinase digestion. The carboxyl-terminal domain bound to laminarin, a beta-1,3-glucan with beta-1,6 branches, but not to curdlan, a beta-1,3-glucan that lacks branching. These results indicate that the two domains of Plodia beta-1,3-glucan recognition protein, separated by a putative linker region, bind microbial polysaccharides with differing specificities and that the amino-terminal domain, which is unique to this class of pattern recognition receptors from invertebrates, is responsible for stimulating prophenoloxidase activation.
Fabrick
Jeffrey A
JA
Department of Biochemistry, Kansas State University, Manhattan, Kansas 66506, USA.
Baker
James E
JE
Kanost
Michael R
MR
D000818:Animals; D002942:Circular Dichroism; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D004926:Escherichia coli / Q000378:metabolism; D017353:Gene Deletion; D005936:Glucans / Q000737:chemistry*; D007113:Immunity, Innate; D008070:Lipopolysaccharides / Q000737:chemistry; D009036:Moths; D011134:Polysaccharides / Q000737:chemistry; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry; D020349:Surface Plasmon Resonance; D013682:Teichoic Acids; D013696:Temperature; D013997:Time Factors; D047071:beta-Glucans
D018076:DNA, Complementary; D005936:Glucans; D008070:Lipopolysaccharides; D011134:Polysaccharides; D011994:Recombinant Proteins; D013682:Teichoic Acids; D047071:beta-Glucans; C009900:lipoteichoic acid; C038459:curdlan; C033363:beta-1,3-glucan