title: [Cloning and sequence analysis of the genome of a strain of duck hepatitis B virus].
abstract: OBJECTIVE
  To clone and analyze the genome of a duck hepatitis B virus (DHBV) strain isolated from the ducks found in the local area.
  
  
  METHODS
  The complete genome of DHBV was amplified by PCR prior to cloning into T vector and sequencing, with also homology and phylogenetic analyses.
  
  
  RESULTS
  Genome comparison of an isolated DHBV strain and 16 DHBV strains in GenBank revealed a homology of 89.4% to 99.3% at the nucleotide level, and the amino acid identity of the 3 open reading frames showed that the P region harbored more obvious variations than the other regions.
  
  
  CONCLUSIONS
  The isolated DHBV strain might belong to a subtype of the virus found in the western countries.
authors.affiliation: Department of Infectious Diseases, Nangfang Hospital, First Military Medical University, Guangzhou 510515, China.
authors.forename: Wei-fang
authors.initials: WF
authors.lastname: Liang
authors.forename: Zhi-hua
authors.initials: ZH
authors.lastname: Liu
authors.forename: Hai-tang
authors.initials: HT
authors.lastname: He
authors.forename: Kang-xian
authors.initials: KX
authors.lastname: Luo
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000737:chemistry*; D004372:Ducks; D015611:Hepatitis B Virus, Duck / Q000145:classification / Q000235:genetics*; D016366:Open Reading Frames; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D004279:DNA, Viral