[Metabolism of proinflammatory cytokine (interleukin-1 beta) and oxidant activity of neutrophils in various forms of chronic bronchitis].
OBJECTIVE
To study content of interleukin-1 beta (IL-1 beta) and free radical oxidation in neutrophils of blood, saliva and bronchoalveolar lavage (BAL) in chronic bronchitis for prediction of obstructive defects.


METHODS
Enzyme immunoassay was used to measure IL-1 beta in the blood, saliva, BAL of 49 males with chronic bronchitis aged 39-72 years. Functional ability of the neutrophils to generate free oxygen radicals was assessed by luminol-dependent chemiluminescence.


RESULTS
Patients with chronic obstructive bronchitis exhibited excessive accumulation of IL-1 beta in the blood and BAL, enhanced activity of hematosalivary barrier for IL-1 beta and production of active oxygen radicals by systemic and tissue neutrophils.


CONCLUSIONS
Elevated concentration of systemic and tissue IL-1 beta, activation of free radical oxidation by blood neutrophils and BAL, enhancement of hematosalivary barrier for IL-1 beta can be considered as prognostically unfavourable factors of bronchial obstruction in patients with chronic bronchitis.
Postnikova
L B
LB
Alekseeva
O P
OP
Kubysheva
N I
NI
Ishanova
O S
OS
D000328:Adult; D000368:Aged; D029481:Bronchitis, Chronic / Q000097:blood* / Q000276:immunology / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012463:Saliva / Q000276:immunology / Q000378:metabolism
D007375:Interleukin-1; D017382:Reactive Oxygen Species