Insulin and amino acids both strongly participate to the regulation of protein metabolism.
OBJECTIVE
The application of tracer kinetic methods, combined with measurements of the activity of components of the cellular signaling pathways involved in protein synthesis and degradation, affords new insights into the regulation of skeletal muscle protein metabolism in vivo in humans. Feeding is associated with an increase in protein synthesis and a decrease in proteolysis. These changes are mediated by feeding-induced increases in plasma concentrations of both nutrients and hormones.


RESULTS
Recent studies definitely demonstrated that insulin and amino acids directly interacted in promoting postprandial anabolism. However, the contribution of amino acids was abolished in old individuals in whom only insulin action persisted. There was a line of evidence that the effect of amino acids originates from leucine, which should not be viewed simply as a building block for protein synthesis, but as a signal in the regulation of cell functions. Although their cellular signaling pathways do not completely overlap, insulin and amino acids both activate the translation initiation of protein synthesis. Insulin presumably inhibits skeletal muscle protein degradation through a decrease in the activity of the ubiquitin proteasome-dependent pathway.


CONCLUSIONS
Whether or not amino acids modify insulin action and have specific effects on proteolysis has not yet been documented. At the molecular level, amino acids such as insulin modulate gene expression. Such studies are needed to gain a better understanding of the interactions between insulin and amino acids in the regulation of skeletal muscle protein anabolism.
Prod'homme
Magali
M
Protein, Metaboism and Nutrition Unit, INRA, Theix, France.
Rieu
Isabelle
I
Balage
Michele
M
Dardevet
Dominique
D
Grizard
Jean
J
D000328:Adult; D000596:Amino Acids / Q000097:blood / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D007328:Insulin / Q000097:blood / Q000502:physiology*; D018482:Muscle, Skeletal / Q000378:metabolism / Q000502:physiology; D011506:Proteins / Q000378:metabolism*
D000596:Amino Acids; D007328:Insulin; D011506:Proteins