title: Toxic and sub-lethal effects of oleandrin on biochemical parameters of fresh water air breathing murrel, Channa punctatus (Bloch.).
abstract: Active compound oleandrin extracted from Nerium indicum (Lal Kaner) leaf has potent piscicidal activity. The piscicidal activity of oleandrin on freshwater fish C. punctatus was both time and dose dependent. Exposure to sub-lethal doses of oleandrin for 24hr and 96hr to fish caused significant alteration in the level of total protein, total free amino acid, nucleic acid, glycogen, pyruvate, lactate and enzyme protease, phosphatases, alanine aminotransferase, aspartate aminotransferase and acetylcholinesterase activity in liver and muscle tissues. The alterations in all the above biochemical parameters were also significantly time and dose dependent. The results show a significant recovery in all the above biochemical parameters, in both liver and muscle tissues of fish after the 7th day of the withdrawal of treatment. Toxicity persistence test of oleandrin on juvenile Labeo rohita shows that fish seed of common culturing carp can be released into rearing ponds after three days of oleandrin treatment. It supports the view that the oleandrin is safer and may be useful substitute of other piscicides for removing the unwanted freshwater fishes from aquaculture ponds.
authors.affiliation: Natural Product Laboratory, Department of Zoology, D.D.U. Gorakhpur University, Gorakhpur 273009, India.
authors.forename: Sudhanshu
authors.initials: S
authors.lastname: Tiwari
authors.forename: Ajay
authors.initials: A
authors.lastname: Singh
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002298:Cardenolides / Q000737:chemistry / Q000633:toxicity*; D002301:Cardiac Glycosides / Q000737:chemistry / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D006003:Glycogen / Q000378:metabolism; D007194:India; D019344:Lactic Acid / Q000378:metabolism; D007928:Lethal Dose 50; D008099:Liver / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D009132:Muscles / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D010473:Perciformes / Q000502:physiology*; D010936:Plant Extracts / Q000633:toxicity; D018515:Plant Leaves / Q000633:toxicity; D019289:Pyruvic Acid / Q000378:metabolism
chemical_list: D002298:Cardenolides; D002301:Cardiac Glycosides; D010936:Plant Extracts; D019344:Lactic Acid; D019289:Pyruvic Acid; D006003:Glycogen; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000110:Acetylcholinesterase; C021065:oleandrin