Tensile forces attenuate estrogen-stimulated collagen synthesis in the ACL.
The purpose of this study was to examine whether mechanical tensile forces affect estrogen regulation of collagen synthesis of anterior cruciate ligament fibroblasts at the mRNA level. Estrogen was studied at three physiologic levels, 10(-11), 10(-10), and 10(-9)M. The results revealed that estrogen alone stimulated Type I and III collagen synthesis at the mRNA level, and application of mechanical force decreased the expression of collagen Type I and III genes at all tested estrogen levels. These findings suggest that estrogen may directly regulate ligament structure and function by alteration of Type I and III collagen synthesis. This regulation is dependent on mechanical loading.
Lee
Chun-Yi
CY
Department of Cell Biology, Baylor College of Medicine, Houston, TX, USA.
Smith
Carolyn L
CL
Zhang
Xiaoliu
X
Hsu
Horng-Chaung
HC
Wang
Der-Yean
DY
Luo
Zong-Ping
ZP
D000818:Animals; D016118:Anterior Cruciate Ligament / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis*; D024061:Collagen Type III / Q000096:biosynthesis*; D004967:Estrogens / Q000494:pharmacology*; D005347:Fibroblasts / Q000378:metabolism; D015870:Gene Expression; D012333:RNA, Messenger / Q000096:biosynthesis; D013314:Stress, Mechanical; D013552:Swine; D013718:Tensile Strength
D024042:Collagen Type I; D024061:Collagen Type III; D004967:Estrogens; D012333:RNA, Messenger