Activation of mouse RAG-2 promoter by Myc-associated zinc finger protein.
Recombination activating gene-1 (RAG-1) and RAG-2 are expressed specifically in lymphocytes undergoing the antigen receptor gene rearrangement during the lymphocyte development. Our previous study showed that the -41 to -17 nucleotides (nt) 5' -upstream region of mouse RAG-2 were pre-requisite for the core promoter activity and that Pax-5/c-Myb/LEF-1 protein-protein complex was responsible for its activity in immature B cells. In this study, we show that the -65/-42 sequence, the non-conserved sequence between human and mouse RAG-2 promoter, is necessary for the full promoter activity for mouse RAG-2. Electrophoresis mobility shift assay revealed that Myc-associated zinc finger protein (MAZ) as well as SP1/3 binds a GA box in this region. Using chromatin immunoprecipitation, we show that MAZ binds the RAG-2 promoter region in pre-B cells. Furthermore, we show that MAZ synergistically activates the murine RAG-2 promoter with Pax-5/c-Myb/LEF-1 complex. These results first demonstrate that MAZ participates in activation of mouse RAG-2 promoter.
Wu
Chun-Xiao
CX
Department of Immunology, Faculty of Medicine, Toyama Medical and Pharmaceutical University, 2630, Sugitani, Toyama 930-0194, Japan.
Zhao
Wen-Pu
WP
Kishi
Hiroyuki
H
Dokan
Junichi
J
Jin
Zhe-Xiong
ZX
Wei
Xing-Cheng
XC
Yokoyama
Kazunari K
KK
Muraguchi
Atsushi
A
D000818:Animals; D016175:B-Lymphocyte Subsets / Q000166:cytology / Q000378:metabolism; D001482:Base Composition; D001483:Base Sequence; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D008156:Luciferases / Q000235:genetics; D051379:Mice; D041681:NIH 3T3 Cells; D009687:Nuclear Proteins; D011401:Promoter Regions, Genetic / Q000235:genetics; D020598:Proto-Oncogene Proteins c-myb / Q000737:chemistry / Q000502:physiology*; D016415:Sequence Alignment; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection; D016335:Zinc Fingers / Q000235:genetics / Q000502:physiology*
D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D009687:Nuclear Proteins; D020598:Proto-Oncogene Proteins c-myb; C499311:RAG2 protein, human; C499308:Rag2 protein, mouse; D014157:Transcription Factors; C064655:V(D)J recombination activating protein 2; C064658:RAG-1 protein; D008156:Luciferases