title: Golgi targeting of ARF-like GTPase Arl3p requires its Nalpha-acetylation and the integral membrane protein Sys1p.
abstract: Myristoylation of ARF family GTPases is required for their association with Golgi and endosomal membranes, where they regulate protein sorting and the lipid composition of these organelles. The Golgi-localized ARF-like GTPase Arl3p/ARP lacks a myristoylation signal, indicating that its targeting mechanism is distinct from myristoylated ARFs. We demonstrate that acetylation of the N-terminal methionine of Arl3p requires the NatC N(alpha)-acetyltransferase and that this modification is required for its Golgi localization. Chemical crosslinking and fluorescence microscopy experiments demonstrate that localization of Arl3p also requires Sys1p, a Golgi-localized integral membrane protein, which may serve as a receptor for acetylated Arl3p.
authors.affiliation: Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, 421 Curie Blvd. BRB 2/3 room 1010, Philadelphia, PA 19104-6058, USA.
authors.forename: Subba Rao Gangi
authors.initials: SR
authors.lastname: Setty
authors.forename: Todd I
authors.initials: TI
authors.lastname: Strochlic
authors.forename: Amy Hin Yan
authors.initials: AH
authors.lastname: Tong
authors.forename: Charles
authors.initials: C
authors.lastname: Boone
authors.forename: Christopher G
authors.initials: CG
authors.lastname: Burd
mesh_terms: D020727:ADP-Ribosylation Factors / Q000235:genetics / Q000378:metabolism*; D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism*; D001191:Arylamine N-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D063210:N-Terminal Acetyltransferase B; D021381:Protein Transport / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D033921:Vesicular Transport Proteins
chemical_list: C077082:MAK10 protein, S cerevisiae; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; C444081:Sys1 protein, S cerevisiae; D033921:Vesicular Transport Proteins; D000123:Acetyltransferases; D063210:N-Terminal Acetyltransferase B; C077299:MAK3 protein, S cerevisiae; D001191:Arylamine N-Acetyltransferase; D020727:ADP-Ribosylation Factors; C490249:ARL3 protein, S cerevisiae