title: Recoverin as a paraneoplastic antigen in lung cancer: the occurrence of anti-recoverin autoantibodies in sera and recoverin in tumors.
abstract: Using immunoblotting with recombinant recoverin as an antigen, we have examined 279 serum samples from individuals with small cell lung carcinoma (SCLC, 99 patients), non-small cell lung carcinoma (NSCLC, 44 patients), and non-malignant pulmonary disorders (86 patients) as well as sera from 50 healthy donors. Autoantibodies against recoverin (anti-Rc) were detected in sera from 15 patients with SCLC (15% of cases) and from 9 patients with NSCLC (about 20% of cases). Only two anti-Rc positive cases were detected in patients with non-malignant pulmonary disorders, while no such cases were found in healthy individuals. Immunohistochemical investigation of paraffin sections of 44 SCLC and 40 NSCLC tumors revealed recoverin-positive reaction in 30 SCLC (68%) and 34 NSCLC (85%) sections. Despite the high specificity (98%), the low sensitivity (less than 20%) does not allow serum anti-Rc to be considered as a valuable marker of lung cancer. However, taking into account the high occurrence of aberrant expression of recoverin in lung tumors, this PNA could be considered as a potential target for immunotherapy of lung cancer.
authors.affiliation: Laboratory of Biomedicine, Department of Cell Signalling, A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia.
authors.forename: Alexandr V
authors.initials: AV
authors.lastname: Bazhin
authors.forename: Marina S
authors.initials: MS
authors.lastname: Savchenko
authors.forename: Olga N
authors.initials: ON
authors.lastname: Shifrina
authors.forename: Sofia A
authors.initials: SA
authors.lastname: Demoura
authors.forename: Svetlana Yu
authors.initials: SY
authors.lastname: Chikina
authors.forename: Gabriele
authors.initials: G
authors.lastname: Jaques
authors.forename: Eugenia A
authors.initials: EA
authors.lastname: Kogan
authors.forename: Alexandr G
authors.initials: AG
authors.lastname: Chuchalin
authors.forename: Pavel P
authors.initials: PP
authors.lastname: Philippov
mesh_terms: D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis*; D001323:Autoantibodies / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D002135:Calcium-Binding Proteins / Q000032:analysis*; D002289:Carcinoma, Non-Small-Cell Lung / Q000276:immunology*; D018288:Carcinoma, Small Cell / Q000276:immunology*; D005136:Eye Proteins; D005260:Female; D051597:Hippocalcin; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D008074:Lipoproteins; D008171:Lung Diseases / Q000276:immunology*; D008175:Lung Neoplasms / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins; D051603:Recoverin; D012680:Sensitivity and Specificity
chemical_list: D000951:Antigens, Neoplasm; D001323:Autoantibodies; D014408:Biomarkers, Tumor; D002135:Calcium-Binding Proteins; D005136:Eye Proteins; D008074:Lipoproteins; D009419:Nerve Tissue Proteins; C496731:RCVRN protein, human; D051603:Recoverin; D051597:Hippocalcin