[Determination of protein allergenicity--BN rat model].
OBJECTIVE
Protein allergenicity was investigated in BN rat model.


METHODS
OVA or BSA was administered i.p. (1 mg/ml) or orally (10 mg/ml) to male BN rats for 6 weeks. In different intervals, blood samples were obtained from the orbital plexus for PCA and histamine determination. In addition, blood pressure of rats in OVA-treated group and control group was measured.


RESULTS
Relatively high titres (1/32-1/128) IgE responses were provoked by administration of OVA, while BSA stimulated only low titres (1/2-1/4) IgE. Compared with the controls, the plasma histamine concentrations of OVA groups were higher. An oral challenge with OVA did not induce a clear effect on blood pressure in the majority of rats. However, two rats demonstrated a temporary drop in blood pressure.


CONCLUSIONS
BN rat model may be a suitable animal model to determine protein allergenicity.
Jia
Xudong
X
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Li
Ning
N
Wang
Wei
W
Wu
Yongning
Y
D000818:Animals; D004044:Dietary Proteins / Q000009:adverse effects*; D005512:Food Hypersensitivity / Q000209:etiology; D006632:Histamine / Q000097:blood*; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D010047:Ovalbumin / Q000009:adverse effects*; D010323:Passive Cutaneous Anaphylaxis; D011897:Random Allocation; D051381:Rats; D011914:Rats, Inbred BN; D012710:Serum Albumin, Bovine / Q000009:adverse effects*
D004044:Dietary Proteins; D012710:Serum Albumin, Bovine; D007073:Immunoglobulin E; D006632:Histamine; D010047:Ovalbumin