Porcine choroid plexus epithelial cells induce Streptococcus suis bacteriostasis in vitro.
The involvement of the choroid plexus in host defense during bacterial meningitis is unclear. Aiming to elucidate possible antibacterial mechanisms, we stimulated primary porcine choroid plexus epithelial cells (pCPEC) with proinflammatory cytokines and challenged them with various Streptococcus suis strains. In the supernatant of gamma interferon (IFN-gamma)-stimulated pCPEC, streptococcal growth was markedly suppressed. Costimulation with tumor necrosis factor alpha enhanced this bacteriostatic effect, while supplementation of L-tryptophan completely eliminated it. We also demonstrate that an activation of indoleamine 2,3-dioxygenase in the pCPEC seems to be responsible for the IFN-gamma-induced bacteriostasis. This supports the hypothesis of an active role of the choroid plexus in host defense against bacterial meningitis.
Adam
Rüdiger A
RA
Klinik für Allgemeine Pädiatrie. Institut für Medizinische Mikrobiologie, Universitätsklinikum Düsseldorf, Düsseldorf, Germany.
Tenenbaum
Tobias
T
Valentin-Weigand
Peter
P
Laryea
Maurice
M
Schwahn
Bernd
B
Angelow
Susanne
S
Galla
Hans-Joachim
HJ
Däubener
Walter
W
Schroten
Horst
H
D000818:Animals; D002831:Choroid Plexus / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D004789:Enzyme Activation; D004847:Epithelial Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000494:pharmacology; D016920:Meningitis, Bacterial / Q000276:immunology; D011994:Recombinant Proteins / Q000494:pharmacology; D013290:Streptococcal Infections / Q000276:immunology; D017011:Streptococcus suis / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D034421:Sus scrofa; D014364:Tryptophan / Q000378:metabolism; D014366:Tryptophan Oxygenase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D014364:Tryptophan; D014366:Tryptophan Oxygenase