title: Art and artefacts of fluorescence correlation spectroscopy.
abstract: Fluorescence correlation spectroscopy (FCS) is an important technique for studying low concentrations of analyte molecules in solution. The core molecular characteristic that can be addressed by FCS is the translational diffusion coefficient of the analyte molecules, which can be used for i.e. studying molecular binding and reactions, or conformational changes of macromolecules. The present paper discusses several possible optical and photophysical effects that can influence the outcome of a FCS measurement and thus can bias the value of the derived diffusion coefficient.
authors.affiliation: Institute for Biological Information Processing 1 & 2, Forschungszentrum Jülich, D-52425 Jülich, Germany. j.enderlein@fz-juelich.de
authors.forename: Jörg
authors.initials: J
authors.lastname: Enderlein
authors.forename: Ingo
authors.initials: I
authors.lastname: Gregor
authors.forename: Digambara
authors.initials: D
authors.lastname: Patra
authors.forename: Jörg
authors.initials: J
authors.lastname: Fitter
mesh_terms: D016477:Artifacts; D004058:Diffusion; D018613:Microscopy, Confocal / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000379:methods*