title: Effect of antisense peptide binding on the dimerization of human cystatin C--gel electrophoresis and molecular modeling studies.
abstract: Human cystatin C (HCC) shows a tendency to dimerize. This process is particularly easy in the case of the L68Q HCC mutant and might lead to formation of amyloid deposits in brain arteries of young adults. Our purpose was to find ligands of monomeric HCC that can prevent its dimerization. Eleven antisense peptide ligands of monomeric HCC were designed and synthesized. The influence of these ligands on HCC dimerization was studied using gel electrophoresis and molecular modeling methods. The results suggest that all the designed peptides interact with monomeric HCC facilitating its dimerization rather than preventing it.
authors.affiliation: Faculty of Chemistry, University of Gdańsk, Gdańsk, Poland. krysias@chemik.chem.univ.gda.pl
authors.forename: Krystyna
authors.initials: K
authors.lastname: Stachowiak
authors.forename: Sylwia
authors.initials: S
authors.lastname: Rodziewicz-Motowidło
authors.forename: Renata
authors.initials: R
authors.lastname: Sosnowska
authors.forename: Franciszek
authors.initials: F
authors.lastname: Kasprzykowski
authors.forename: Leszek
authors.initials: L
authors.lastname: Łankiewicz
authors.forename: Anders
authors.initials: A
authors.lastname: Grubb
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Grzonka
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000682:Amyloid / Q000378:metabolism; D016375:Antisense Elements (Genetics) / Q000737:chemistry*; D001483:Base Sequence; D001665:Binding Sites; D002536:Cerebral Arteries / Q000378:metabolism / Q000473:pathology; D055316:Cystatin C / Q000737:chemistry* / Q000235:genetics; D015891:Cystatins / Q000737:chemistry; D019281:Dimerization; D006801:Humans; D008024:Ligands; D008958:Models, Molecular; D011487:Protein Conformation; D055815:Young Adult
chemical_list: D000682:Amyloid; D016375:Antisense Elements (Genetics); D055316:Cystatin C; D015891:Cystatins; C085910:L68Q cystatin C; D008024:Ligands