title: Efficient intracellular delivery of a protein and a low molecular weight substance via recombinant polyomavirus-like particles.
abstract: Efficient encapsulation of foreign molecules like proteins and low molecular weight drugs into polyoma virus-like particles (capsoids) was achieved by the development of an anchoring technique based upon the specific interaction of the inner core protein VP2 with VP1 pentamers. A stretch of 49 amino acids of VP2 served as an anchor molecule, either expressed as a fusion protein with green fluorescent protein (GFP) or covalently linked to methotrexate (MTX). The loaded capsoids showed regular morphology and stability for several months. GFP and MTX were internalized into cells in vitro, as was demonstrated by the detection of GFP and VP1 fluorescence in mouse fibroblasts and the cytostatic effect of intracellularly released MTX on leukemia T cells.
authors.affiliation: Responsif GmbH, Erlangen, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Abbing
authors.forename: Ulrich K
authors.initials: UK
authors.lastname: Blaschke
authors.forename: Swen
authors.initials: S
authors.lastname: Grein
authors.forename: Michael
authors.initials: M
authors.lastname: Kretschmar
authors.forename: Christoph M B
authors.initials: CM
authors.lastname: Stark
authors.forename: Michael J W
authors.initials: MJ
authors.lastname: Thies
authors.forename: Jürgen
authors.initials: J
authors.lastname: Walter
authors.forename: Martina
authors.initials: M
authors.lastname: Weigand
authors.forename: Diemuth C
authors.initials: DC
authors.lastname: Woith
authors.forename: Jürgen
authors.initials: J
authors.lastname: Hess
authors.forename: Christian O A
authors.initials: CO
authors.lastname: Reiser
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D036022:Capsid Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000493:pharmacokinetics*; D002470:Cell Survival / Q000187:drug effects; D004355:Drug Stability; D004926:Escherichia coli / Q000235:genetics / Q000821:virology; D005454:Fluorescence Polarization; D049452:Green Fluorescent Proteins; D007962:Leukocytes / Q000378:metabolism; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics; D008727:Methotrexate / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011120:Polyomavirus / Q000737:chemistry* / Q000235:genetics; D011233:Precipitin Tests; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D036022:Capsid Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; C062481:VP1 protein, polyomavirus; D049452:Green Fluorescent Proteins; D008727:Methotrexate