title: A subtractive approach to characterize genes with regionalized expression in the gliogenic ventral neuroepithelium: identification of chick sulfatase 1 as a new oligodendrocyte lineage gene.
abstract: To address the question of the origin of glial cells and the mechanisms leading to their specification, we have sought to identify novel genes expressed in glial progenitors. We adopted suppression subtractive hybridization (SSH) to establish a chick cDNA library enriched for genes specifically expressed at 6 days of incubation (E6) in the ventral neuroepithelium, a tissue previously shown to contain glial progenitors. Screens were then undertaken to select differentially expressed cDNAs, and out of 82 unique SSH clones, 21 were confirmed to display a regionalized expression along the dorsoventral axis of the E6 ventral neuroepithelium. Among these, we identified a transcript coding for the chick orthologue of Sulf1, a recently identified cell surface sulfatase, as a new, early marker of oligodendrocyte (OL) precursors in the chick embryonic spinal cord. This study provides groundwork for the further identification of genes involved in glial specification.
authors.affiliation: Centre de Biologie du Développement, UMR5547 CNRS/UPS, Université Paul Sabatier, 31062 Toulouse Cedex, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Braquart-Varnier
authors.forename: Cathy
authors.initials: C
authors.lastname: Danesin
authors.forename: Catherine
authors.initials: C
authors.lastname: Clouscard-Martinato
authors.forename: Eric
authors.initials: E
authors.lastname: Agius
authors.forename: Nathalie
authors.initials: N
authors.lastname: Escalas
authors.forename: Bertrand
authors.initials: B
authors.lastname: Benazeraf
authors.forename: Xingbin
authors.initials: X
authors.lastname: Ai
authors.forename: Charles
authors.initials: C
authors.lastname: Emerson
authors.forename: Philippe
authors.initials: P
authors.lastname: Cochard
authors.forename: Cathy
authors.initials: C
authors.lastname: Soula
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D019521:Body Patterning / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics; D002490:Central Nervous System / Q000166:cytology / Q000196:embryology* / Q000201:enzymology; D002642:Chick Embryo; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D004847:Epithelial Cells / Q000166:cytology / Q000201:enzymology*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D015698:Genomic Library; D009419:Nerve Tissue Proteins / Q000235:genetics; D009693:Nucleic Acid Hybridization / Q000379:methods; D009836:Oligodendroglia / Q000166:cytology / Q000201:enzymology*; D013234:Stem Cells / Q000166:cytology / Q000201:enzymology*; D015238:Sulfotransferases / Q000235:genetics*
chemical_list: D015415:Biomarkers; D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; C470791:SULF1 protein, human; D015238:Sulfotransferases