Programmable modification of cell adhesion and zeta potential in silica microchips.
Spatial patterning of thin polyacrylamide films bonded to self-assembled monolayers on silica microchannels is described as a means for manipulating cell-adhesion and electroosmotic properties in microchips. Streaming potential measurements indicate that the zeta potential is reduced by at least two orders of magnitude at biological pH, and the adhesion of several kinds of cells is reduced by 80-100%. Results are shown for cover slides and in wet-etched silica microchannels. Because the polyacrylamide film is thin and transparent, this film is consistent with optical manipulation of cells and detection of cell contents. The spatial patterning technique is straightforward and has the potential to aid on-chip analysis of single adherent cells.
Kirby
Brian J
BJ
Microfluidics Department, Sandia National Laboratories, Livermore, CA, USA. bjkirby@sandia.gov
Wheeler
Aaron R
AR
Zare
Richard N
RN
Fruetel
Julia A
JA
Shepodd
Timothy J
TJ
D000180:Acrylic Resins / Q000737:chemistry*; D002448:Cell Adhesion; D002478:Cells, Cultured; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D000099166:Polyacrylamides
D000180:Acrylic Resins; D012822:Silicon Dioxide; D000099166:Polyacrylamides