[Application of dot-immunogold filtration assay in detection of IgG in sera of trichinellosis patients].
OBJECTIVE
To study a new quick method for detecting serum IgG against Trichinella spiralis (T.s).


METHODS
Membrane antigen of muscle larva of T.s was isolated and combined with the nitrocellulose membrane. Goat anti-human IgG was conjugated with the golden pellets of colloidal state which was used as marked antibody for the experiment of dot-immunogold filtration assay to examine specific IgG.


RESULTS
T.s IgG and its corresponding antigen reacted on the membrane by filtration, and the result could be observed with naked eyes within 10 min. In the examination of 76 clinical serum samples, the positive rate was 94.7%. It was not significantly higher than that of ELISA (chi 2 = 2.83, P > 0.05).


CONCLUSIONS
The dot-immunogold filtration assay is rapid and simple for performing and reading in the detection of IgG against T.s with high sensitivity and specificity.
Liu
Ying-jie
YJ
Cellular and Molecular Immunology, Laboratory of Henan University, Kaifeng 475001.
Liu
Yun
Y
Qu
Yao-hua
YH
Zheng
Yong
Y
Liu
Yan-hong
YH
Zhang
Yong-li
YL
Chen
Juan
J
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood; D012680:Sensitivity and Specificity; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000175:diagnosis* / Q000276:immunology
D000909:Antibodies, Helminth; D007074:Immunoglobulin G