title: [Genotyping of HBV DNA in Shenzhen and clinical manifestations].
abstract: OBJECTIVE
  To detect HBV DNA and its genotypes.
  
  
  METHODS
  The 6 isoforms of HBV DNA was detected out using of different probes by Polymerase Chain Reaction and Nucleic Acid hybridization.
  
  
  RESULTS
  Of 150 HBV DNA positive patients who lived in Shenzhen, 50 samples (33%) are type B, 36 samples (24%) are type C, 13 samples (9%) are type D, 3 samples is type F, 1 sample is type A, 48 samples (31%) are mixed type. The ALT value was significantly higher in genotype B than in genotype C. HBe positivity were higher in genotype B than genotype C. HBeAg positivity were higher in genotype C than in genotype B. There are not obvious relations between genotype and age or sex.
  
  
  CONCLUSIONS
  In the detected samples, the major genotype of HBV DNA is type B, several are type C, D. The type E haven't been found. There are some relations between all kinds of genotypes and the severity of hepatitis B.
authors.affiliation: The Department of Digestion Medicine of Shenzhen People's Hospital, Shenzhen 518020, China.
authors.forename: Jian-yi
authors.initials: JY
authors.lastname: Dai
authors.forename: Zhi-lin
authors.initials: ZL
authors.lastname: Shi
authors.forename: Yong
authors.initials: Y
authors.lastname: Dai
authors.forename: Hong
authors.initials: H
authors.lastname: Du
authors.forename: De-heng
authors.initials: DH
authors.lastname: Chen
authors.forename: Sha-yan
authors.initials: SY
authors.lastname: Wang
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005838:Genotype; D006509:Hepatitis B / Q000821:virology*; D006515:Hepatitis B virus / Q000145:classification* / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral