Striatal PSA-NCAM(+) precursor cells from the newborn rat express functional glycine receptors.
Immunocytochemical analysis showed that ionotropic glycine receptors are expressed in neurogenic progenitors purified from the newborn rat striatum and expressing the polysialylated form of the neural cell adhesion molecule, both in vitro and in situ. To ascertain whether glycine receptors were functional in vitro, whole-cell patch-clamp recordings demonstrated that glycine triggers inward strychnine-sensitive currents in the majority of these cells. Moreover, we found that glycine receptors expressed by these neurogenic progenitors display intermediate electrophysiological characteristics between those of glycine receptors expressed by neural stem cells and by mature interneurons from the rat striatum. Altogether, the present data show that functional strychnine-sensitive glycine receptors are expressed in neurogenic progenitors purified from the newborn rat striatum.
Nguyen
Laurent
L
Center for Cellular and Molecular Neurobiology, University of Liège, 17 Place Delcour, B-4020 Liège, Belgium. lnguyen@nimr.mrc.ac.uk
Malgrange
Brigitte
B
Breuskin
Ingrid
I
Lallemend
François
F
Hans
Grégory
G
Moonen
Gustave
G
Belachew
Shibeshih
S
Rigo
Jean-Michel
JM
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000166:cytology / Q000378:metabolism*; D005998:Glycine / Q000494:pharmacology; D007150:Immunohistochemistry; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D039842:Neural Cell Adhesion Molecule L1 / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018009:Receptors, Glycine / Q000187:drug effects / Q000378:metabolism*; D012794:Sialic Acids / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013331:Strychnine / Q000494:pharmacology
D039842:Neural Cell Adhesion Molecule L1; D018009:Receptors, Glycine; D012794:Sialic Acids; C103469:polysialyl neural cell adhesion molecule; D013331:Strychnine; D005998:Glycine