Adenovirus-mediated delivery of GDNF ameliorates corticospinal neuronal atrophy and motor function deficits in rats with spinal cord injury.
The aim of the present study was to assess the effect of glial cell line-derived neurotrophic factor (GDNF) delivery mediated by a recombinant adenovirus (AdCMVgdnf or AdCMVlacZ) on the functional recovery and central neuronal atrophy in adult rats with spinal cord injury. Bilateral electrolytic lesions were made in the corticospinal tracts at the T10 vertebral level in a rat model. AdCMVgdnf or AdCMVlacZ was injected at the lesion site immediately after operation. After 2-3 weeks the neurological score and the inclined plane angle were significantly higher and the soma size of corticospinal motoneurons was larger in the AdCMVgdnf group compared with the AdCMVlacZ group. These results demonstrated that adenovirus-mediated delivery of GDNF could prevent the retrograde atrophy of corticospinal motoneurons and improve the motor function in rats with spinal cord injury.
Tang
Xiao-Qing
XQ
Neuroscience Research Institute, Department of Neurobiology, Peking University Health Science Center, 38 Xueyuan Road, Beijing 100083, PR China.
Wang
Yun
Y
Huang
Zhi-Hua
ZH
Han
Ji-Sheng
JS
Wan
You
Y
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D001284:Atrophy; D001522:Behavior, Animal / Q000502:physiology; D005260:Female; D015316:Genetic Therapy; D051100:Glial Cell Line-Derived Neurotrophic Factor; D007763:Lac Operon / Q000235:genetics; D009046:Motor Neurons / Q000473:pathology; D009069:Movement Disorders / Q000209:etiology* / Q000628:therapy*; D009414:Nerve Growth Factors / Q000096:biosynthesis / Q000235:genetics*; D011712:Pyramidal Tracts / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013119:Spinal Cord Injuries / Q000150:complications* / Q000628:therapy*; D013238:Stereotaxic Techniques
C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors