title: Autonomous multielectrode system for monitoring the interactions of isoflavonoids with lung cancer cells.
abstract: The cytotoxic effect of isoflavonoids in the development of different forms of cancer has been reported by epidemiological and dietary studies. Consequently, there is a search for an accurate and reliable method for monitoring the interactions of these chemicals with cancerous cells. We have developed and optimized a fully autonomous electrochemical biosensor for studying the role of isoflavonoids on A549 lung adenocarcinoma cell line. This advanced biosensor uses a prototype 96-electrode (DOX-96) well-type device that allows the measurement of cell respiratory activity via the consumption of dissolved oxygen. The system provides a continuous, real-time monitoring of cell activity upon exposure to naturally occurring polyphenols, specifically resveratrol, genistein, and quercetin. The system is equipped with a multipotentiostat, a 96-electrode well for measurements and cell culturing with 3 disposable electrodes fitted into each well. A comparison with classical "cell culture" techniques indicates that the biosensor provides real-time measurement with no added reagents. A detection limit of 1 x 10(4) was recorded versus 200 and 6 x 10(3) cells/well for MTT and fluorescence assays, respectively. This method was optimized with respect to cell stability, reproducibility, applied potential, cell density per well, volume/composition of cell culture medium per well, and incubation. Others include total measuring time, temperature, and sterilization procedure. This study represents a basic research tool that may allow researchers to study the type, level, and specific influence of isoflavonoids on cells.
authors.affiliation: Department of Chemistry, State University of New York-Binghamton, P.O. Box 6000, Binghamton, New York 13902, USA.
authors.forename: Silvana
authors.initials: S
authors.lastname: Andreescu
authors.forename: Omowunmi A
authors.initials: OA
authors.lastname: Sadik
authors.forename: Dennis W
authors.initials: DW
authors.lastname: McGee
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Suye
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D004566:Electrodes; D005419:Flavonoids / Q000494:pharmacology; D006801:Humans; D007529:Isoflavones / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D010636:Phenols / Q000494:pharmacology; D059808:Polyphenols; D013050:Spectrometry, Fluorescence / Q000379:methods; D013696:Temperature; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D005419:Flavonoids; D007529:Isoflavones; D010636:Phenols; D059808:Polyphenols