title: Determination and characterization of chloroguaiacol conjugates in fish bile by HPLC.
abstract: In this study a small-scale technique for direct analysis of metabolic conjugates of 4,5,6-trichloro- (CG-3) and tetrachloroguaiacols (CG-4) in fish bile by RP-HPLC is presented. Only one metabolite, glucuronic acid conjugate, was demonstrated in two Lake Baikal fishes (Leuciscus leuciscus baikalensis and Cottus kessleri) exposed to CG-3 or CG-4 at 6 degrees C for 1-2 days. In Leuciscus the ratio between free CG-4 in the ambient water and conjugated CG-4 in the bile averaged 264,000. Intraperitoneal injections of tetrachloroveratrol (CV-4) into Thymallus arcticus baikalensis did not reveal any traces of free CV-4 or CG-4 glucuronide in the bile in 2 days at 5 degrees C. We therefore suggest that CV-4 was not demethylated and subsequently conjugated with glucuronic acid in the liver of this fish species.
authors.affiliation: Department of Biology, University of Joensuu, PO Box 111, SF-80101 Joensuu, Finland.
authors.forename: A O
authors.initials: AO
authors.lastname: Oikari
authors.forename: G I
authors.initials: GI
authors.lastname: Baram
authors.forename: V K
authors.initials: VK
authors.lastname: Evstafyev
authors.forename: M A
authors.initials: MA
authors.lastname: Grachev