Modulation of intracellular Na+ concentration by BmK AS, a scorpion toxin, in B104 cell line.
ABSL Effects of BmK AS, a toxin from scorpion Buthus mortensi Karsch and an activator of skeletal muscle RyRs, on the intracellular Na+ concentration have been investigated in the B104 neuroblastoma cell line by fluorescence digital imaging techniques. The intracellular Na+ concentration was elevated by 500 nM BmK AS significantly over a 30 min period, and the effect could be enhanced by addition of ouabain. In addition, BmK AS induced a biphasic modulation of [Na+]i in the dose-dependent range from 20 to 500 nM in the presence of ouabain. The results suggest that BmK AS may modulate intracellular signals by altering the intracellular Na+ concentration in the B104 cells.
Tan
Zhi-Yong
ZY
Institute of Physiology, Shanghai Institute of Biological Science, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, PR China.
Chen
Jin
J
Feng
Xing-Hua
XH
Susumu
Terakawa
T
Ji
Yong-Hua
YH
D000818:Animals; D002414:Cations, Monovalent / Q000378:metabolism; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D009474:Neurons / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D012604:Scorpion Venoms / Q000494:pharmacology*; D012964:Sodium / Q000378:metabolism*
C119407:BmK AS polypeptide; D002414:Cations, Monovalent; D010455:Peptides; D012604:Scorpion Venoms; D012964:Sodium