title: Evaluation of the enantiomeric selectivity in the chiral ligand-exchange chromatography of amino acids by a computational model.
abstract: The chromatographic resolution of enantiomeric amino acids is accomplished on a reversed phase column using aqueous mobile phase containing the chiral reagent N,N-dimethyl-S-phenylalanine-Cu(II). The separation is a result of the whole interaction between the diastereomeric complex surface and the mixed stationary phase realized by the dynamic coating of the RP-18 carbon chains layer. The elution order seems to be related to the different water coordination capability on copper ion in the formation of the mixed ternary complexes.
authors.affiliation: Dipartimento di Chimica e Tecnologia del Farmaco, Università degli Studi di Perugia, Via del Liceo 1, 06123 Perugia, Italy. natalini@iris.chimfarm.unipg.it
authors.forename: Benedetto
authors.initials: B
authors.lastname: Natalini
authors.forename: Maura
authors.initials: M
authors.lastname: Marinozzi
authors.forename: Roccaldo
authors.initials: R
authors.lastname: Sardella
authors.forename: Antonio
authors.initials: A
authors.lastname: Macchiarulo
authors.forename: Roberto
authors.initials: R
authors.lastname: Pellicciari
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D008024:Ligands; D008958:Models, Molecular; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D008024:Ligands