Preliminary X-ray analysis of a human V(H) fragment at 1.8 A resolution.
Small antibody fragments are more useful than full-size antibodies for achieving efficient biodistribution. As a first step towards the design of a clinically desirable antibody fragment, the crystallization of a human V(H) fragment has been achieved. The fragment was derived from the single-chain antibody scFvM12, which recognizes a cancer-specific hypoglycosylated form of mucin. The V(H) fragment was obtained by in-drop digestion of the scFvM12 with a low concentration of the broad-spectrum protease subtilisin Carlsberg. The crystal belongs to the monoclinic space group C2. The crystal diffracted to 1.8 A resolution when analysed at 100 K using a rotating-anode X-ray generator.
Gaur
Rajneesh Kumar
RK
Institute for Molecular Biotechnology VII, Aachen University, Worringerweg 1, 52074 Aachen, Germany.
Kupper
Michael B
MB
Fischer
Rainer
R
Hoffmann
Kurt M V
KM
D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D006801:Humans; D007128:Immunoglobulin Fragments / Q000737:chemistry*; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry*; D007135:Immunoglobulin Variable Region / Q000737:chemistry*; D008969:Molecular Sequence Data; D011487:Protein Conformation
D007128:Immunoglobulin Fragments; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region