Volume-dependent regulation of Ca2+-activated potassium channels in erythrocytes from healthy donors and patients with type II diabetes mellitus aggravated by arterial hypertension.
Increase in intracellular Ca2+ concentration caused by calcium ionophore A23187 or ascorbate+phenazine methosulphate electron donor system added to erythrocyte suspension induced similar shifts in erythrocyte membrane potential. These processes are most likely mediated by Ca2+-activated potassium channels. Changes in the osmolarity of the incubation medium produced opposite effects on membrane hyperpolarization induced by A23187 or ascorbate+phenazine methosulphate in erythrocyte isolated from healthy donors, which attests to the existence of different mechanisms of regulation of Ca2+-activated potassium channels. There was no difference in the volume-dependent changes of potassium permeability in cells from patients with type II diabetes mellitus combined with arterial hypertension induced by application A23187 or electron-donor system.
Kremeno
S V
SV
Siberian State Medical University, Tomsk Research Center, Siberian Division of the Russian Academy of Medical Sciences, Tomsk. liv@cardio.tsu.ru
Petrova
I V
IV
Sitozhevskii
A V
AV
Prokop'eva
V D
VD
Kovalenko
N S
NS
Novitskii
V V
VV
D000001:Calcimycin / Q000494:pharmacology; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000503:physiopathology*; D004910:Erythrocyte Membrane / Q000187:drug effects; D004912:Erythrocytes / Q000502:physiology*; D006801:Humans; D006973:Hypertension / Q000150:complications*; D008564:Membrane Potentials / Q000187:drug effects; D009994:Osmolar Concentration; D024681:Potassium Channels, Calcium-Activated / Q000502:physiology*
D024681:Potassium Channels, Calcium-Activated; D000001:Calcimycin