title: [Laboratory research of the influence of keyouling on the proliferation of human prepuce epidermis cells and condyloma acuminatum cells].
abstract: OBJECTIVE
  To discuss the mechanism of the Chinese medicine Keyouling in the treatment of condyloma axuminatum (CA).
  
  
  METHODS
  Human prepuce epidermis cells and CA cells were primarily cultured and subcultured. We determined the proliferation of human prepuce epidermis cells and CA cells, and observed the influence of Keyouling with different concentrations on the proliferation of human prepuce epidermis cells and CA cells by means of MTT colourimetry assay.
  
  
  RESULTS
  The absorbance was directly proportional to the numbers of human prepuce epidermis cells (r = 0.9850, P < 0.001) and CA cells (r = 0.9892, P < 0.001). Keyouling had no effect on proliferation of the human prepuce epidermis cells, but it had significant inhibition on CA cells. The concentrations of Keyouling bore negative correlation with the proliferation percentage of CA cells(r = -0.4124, P < 0.01).
  
  
  CONCLUSIONS
  Keyouling can significantly restrain the growth and proliferation of CA cells but has no damaging effect on normal organic cuticle cells. It is suggested that Keyouling might have anti-HPV effect.
authors.affiliation: Department of Andrology, General Hospital of Panzhihua Steel & Iron (Group) Co., Panzhihua, Sichuan 617023, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.forename: Shuwu
authors.initials: S
authors.lastname: Zhang
authors.forename: Jiuyuan
authors.initials: J
authors.lastname: Wang
authors.forename: Kaicheng
authors.initials: K
authors.lastname: Wan
authors.forename: Caiming
authors.initials: C
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D002455:Cell Division / Q000187:drug effects; D003218:Condylomata Acuminata / Q000188:drug therapy* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010409:Penile Diseases / Q000188:drug therapy* / Q000473:pathology; D010413:Penis / Q000187:drug effects; D012867:Skin / Q000187:drug effects
chemical_list: D004365:Drugs, Chinese Herbal