Nonlinear interferometric vibrational imaging.
Coherent anti-Stokes Raman scattering (CARS) processes are "coherent," but the phase of the anti-Stokes radiation is lost by most incoherent spectroscopic CARS measurements. We propose a Raman microscopy imaging method called nonlinear interferometric vibrational imaging, which measures Raman spectra by obtaining the temporal anti-Stokes signal through nonlinear interferometry. With a more complete knowledge of the anti-Stokes signal, we show through simulations that a high-resolution Raman spectrum can be obtained of a molecule in a single pulse using broad band radiation. This could be useful for identifying the three-dimensional spatial distribution of molecular species in tissue.
Marks
Daniel L
DL
Beckman Institute for Advanced Science and Technology, Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, 405 North Mathews, Urbana, Illinois 61801, USA.
Boppart
Stephen A
SA
D004247:DNA / Q000737:chemistry; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007368:Interferometry / Q000295:instrumentation / Q000379:methods*; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry; D012313:RNA / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*
D009692:Nucleic Acid Heteroduplexes; D012313:RNA; D004247:DNA