title: Circadian regulation of GABAA receptor function by CKI epsilon-CKI delta in the rat suprachiasmatic nuclei.
abstract: The type A GABA receptors are thought to mediate synchronization of clock cell activity within the suprachiasmatic nuclei (SCN). Here we report that casein kinases I epsilon and delta (CKI epsilon and CKI delta), the crucial clock regulators, form a complex with GABA(A) receptors and inhibit the receptors' function within the SCN according to a circadian rhythm. These results indicate that circadian variation of the kinase-receptor association may mediate regulation of GABA(A) receptor function by CKI epsilon-CKI delta in the SCN.
authors.affiliation: Division of Cellular & Molecular Biology, Toronto Western Research Institute, University Health Network, and Department of Physiology, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Ke
authors.initials: K
authors.lastname: Ning
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Mingxia
authors.initials: M
authors.lastname: Liao
authors.forename: Baosong
authors.initials: B
authors.lastname: Liu
authors.forename: John G
authors.initials: JG
authors.lastname: Mielke
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Duan
authors.forename: Youssef H
authors.initials: YH
authors.lastname: El-Hayek
authors.forename: Qi
authors.initials: Q
authors.lastname: Wan
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D047388:Casein Kinases; D002940:Circadian Rhythm / Q000502:physiology*; D003624:Darkness; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007150:Immunohistochemistry / Q000379:methods; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology; D008029:Lighting / Q000379:methods; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000528:radiation effects; D018408:Patch-Clamp Techniques / Q000379:methods; D010696:Phloroglucinol / Q000031:analogs & derivatives* / Q000494:pharmacology; D011233:Precipitin Tests / Q000379:methods; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000502:physiology*; D018080:Receptors, GABA-B / Q000378:metabolism; D013493:Suprachiasmatic Nucleus / Q000502:physiology*; D013997:Time Factors
chemical_list: D004791:Enzyme Inhibitors; C411652:IC 261; D007211:Indoles; D011963:Receptors, GABA-A; D018080:Receptors, GABA-B; D010696:Phloroglucinol; D011494:Protein Kinases; D047388:Casein Kinases