Antibody response and viraemia during the course of severe acute respiratory syndrome (SARS)-associated coronavirus infection.
To understand the time-course of viraemia and antibody responses to severe acute respiratory syndrome-associated coronavirus (SARS-CoV), RT-PCR and ELISA were used to assay 376 blood samples from 135 SARS patients at various stages of the illness, including samples from patients who were in their early convalescent phase. The results showed that IgM antibodies decreased and became undetectable 11 weeks into the recovery phase. IgG antibodies, however, remained detectable for a period beyond 11 weeks and were found in 100 % of patients in the early convalescent phase. SARS-CoV viraemia mainly appeared 1 week after the onset of illness and then decreased over a period of 1 month, becoming undetectable in the blood samples of the convalescent patients. At the peak of viraemia, viral RNA was detectable in 75 % of blood samples from patients who were clinically diagnosed with SARS 1 or 2 weeks before the test.
Chen
Weijun
W
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China 2Beijing BGI-GBI Biotech Co. Ltd, Beijing 101300, China 3Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 4Beijing 302 Hospital, Beijing 100039, China 5Beijing 301 Hospital, Beijing 100853, China 6Beijing Union Hospital, Beijing 100730, China 7Beijing Red Cross Blood Center, Beijing 100088, China.
Xu
Zuyuan
Z
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China 2Beijing BGI-GBI Biotech Co. Ltd, Beijing 101300, China 3Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 4Beijing 302 Hospital, Beijing 100039, China 5Beijing 301 Hospital, Beijing 100853, China 6Beijing Union Hospital, Beijing 100730, China 7Beijing Red Cross Blood Center, Beijing 100088, China.
Mu
Jingsong
J
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China 2Beijing BGI-GBI Biotech Co. Ltd, Beijing 101300, China 3Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 4Beijing 302 Hospital, Beijing 100039, China 5Beijing 301 Hospital, Beijing 100853, China 6Beijing Union Hospital, Beijing 100730, China 7Beijing Red Cross Blood Center, Beijing 100088, China.
Yang
Ling
L
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China 2Beijing BGI-GBI Biotech Co. Ltd, Beijing 101300, China 3Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 4Beijing 302 Hospital, Beijing 100039, China 5Beijing 301 Hospital, Beijing 100853, China 6Beijing Union Hospital, Beijing 100730, China 7Beijing Red Cross Blood Center, Beijing 100088, China.
Gan
Haixue
H
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China 2Beijing BGI-GBI Biotech Co. Ltd, Beijing 101300, China 3Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 4Beijing 302 Hospital, Beijing 100039, China 5Beijing 301 Hospital, Beijing 100853, China 6Beijing Union Hospital, Beijing 100730, China 7Beijing Red Cross Blood Center, Beijing 100088, China.
Mu
Feng
F
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China 2Beijing BGI-GBI Biotech Co. Ltd, Beijing 101300, China 3Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 4Beijing 302 Hospital, Beijing 100039, China 5Beijing 301 Hospital, Beijing 100853, China 6Beijing Union Hospital, Beijing 100730, China 7Beijing Red Cross Blood Center, Beijing 100088, China.
Fan
Baoxing
B
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China 2Beijing BGI-GBI Biotech Co. Ltd, Beijing 101300, China 3Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 4Beijing 302 Hospital, Beijing 100039, China 5Beijing 301 Hospital, Beijing 100853, China 6Beijing Union Hospital, Beijing 100730, China 7Beijing Red Cross Blood Center, Beijing 100088, China.
He
Bo
B
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China 2Beijing BGI-GBI Biotech Co. Ltd, Beijing 101300, China 3Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 4Beijing 302 Hospital, Beijing 100039, China 5Beijing 301 Hospital, Beijing 100853, China 6Beijing Union Hospital, Beijing 100730, China 7Beijing Red Cross Blood Center, Beijing 100088, China.
Huang
Shengyong
S
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China 2Beijing BGI-GBI Biotech Co. Ltd, Beijing 101300, China 3Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 4Beijing 302 Hospital, Beijing 100039, China 5Beijing 301 Hospital, Beijing 100853, China 6Beijing Union Hospital, Beijing 100730, China 7Beijing Red Cross Blood Center, Beijing 100088, China.
You
Bo
B
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China 2Beijing BGI-GBI Biotech Co. Ltd, Beijing 101300, China 3Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 4Beijing 302 Hospital, Beijing 100039, China 5Beijing 301 Hospital, Beijing 100853, China 6Beijing Union Hospital, Beijing 100730, China 7Beijing Red Cross Blood Center, Beijing 100088, China.
Yang
Yongkui
Y
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China 2Beijing BGI-GBI Biotech Co. Ltd, Beijing 101300, China 3Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 4Beijing 302 Hospital, Beijing 100039, China 5Beijing 301 Hospital, Beijing 100853, China 6Beijing Union Hospital, Beijing 100730, China 7Beijing Red Cross Blood Center, Beijing 100088, China.
Tang
Xiangjun
X
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China 2Beijing BGI-GBI Biotech Co. Ltd, Beijing 101300, China 3Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 4Beijing 302 Hospital, Beijing 100039, China 5Beijing 301 Hospital, Beijing 100853, China 6Beijing Union Hospital, Beijing 100730, China 7Beijing Red Cross Blood Center, Beijing 100088, China.
Qiu
Ling
L
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China 2Beijing BGI-GBI Biotech Co. Ltd, Beijing 101300, China 3Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 4Beijing 302 Hospital, Beijing 100039, China 5Beijing 301 Hospital, Beijing 100853, China 6Beijing Union Hospital, Beijing 100730, China 7Beijing Red Cross Blood Center, Beijing 100088, China.
Qiu
Yan
Y
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China 2Beijing BGI-GBI Biotech Co. Ltd, Beijing 101300, China 3Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 4Beijing 302 Hospital, Beijing 100039, China 5Beijing 301 Hospital, Beijing 100853, China 6Beijing Union Hospital, Beijing 100730, China 7Beijing Red Cross Blood Center, Beijing 100088, China.
Wen
Jie
J
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China 2Beijing BGI-GBI Biotech Co. Ltd, Beijing 101300, China 3Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 4Beijing 302 Hospital, Beijing 100039, China 5Beijing 301 Hospital, Beijing 100853, China 6Beijing Union Hospital, Beijing 100730, China 7Beijing Red Cross Blood Center, Beijing 100088, China.
Fang
Jianqiu
J
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China 2Beijing BGI-GBI Biotech Co. Ltd, Beijing 101300, China 3Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 4Beijing 302 Hospital, Beijing 100039, China 5Beijing 301 Hospital, Beijing 100853, China 6Beijing Union Hospital, Beijing 100730, China 7Beijing Red Cross Blood Center, Beijing 100088, China.
Wang
Jian
J
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China 2Beijing BGI-GBI Biotech Co. Ltd, Beijing 101300, China 3Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 4Beijing 302 Hospital, Beijing 100039, China 5Beijing 301 Hospital, Beijing 100853, China 6Beijing Union Hospital, Beijing 100730, China 7Beijing Red Cross Blood Center, Beijing 100088, China.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D045169:Severe Acute Respiratory Syndrome / Q000276:immunology / Q000503:physiopathology / Q000821:virology; D012720:Severity of Illness Index; D014766:Viremia / Q000276:immunology / Q000503:physiopathology / Q000821:virology
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012367:RNA, Viral