[Expression of MAGE genes and MAGE gene products in human renal and urinary bladder tumor].
OBJECTIVE
To observe the expression of MAGE-1 MAGE-3 genes and MAGE-3 gene product in renal and urinary bladder tumor, and to explore the possibility of MAGE-1 and MAGE-3 genes encoding proteins or MAGE-3 gene product used as a target for immunotherapy in renal and urinary bladder tumor patients.


METHODS
Reverse transcriptase polymerase chain reaction for MAGE-1 and MAGE-3 genes was performed using 39 renal and urinary bladder tumor specimens. Immunohistochemical technique for MAGE-3 antigen was performed using formal infixed paraffin embedded section of 121 renal and urinary bladder tumor specimens.


RESULTS
MAGE-1 and MAGE-3 mRNAs were detected in 23(59.0%) and 22(56.4%) of 39 patients with renal and urinary bladder tumor without expression in 10 tumor surrounding tissues.MAGE-3 antigen was detected in 56(46.3%) of 121 patients with renal and urinary bladder tumor without expression in 10 tumor surrounding tissues. The expression rates of MAGE-1 and MAGE-3 mRNA and MAGE-3 gene product were significantly higher in renal and urinary bladder tumors tissues than in tumor surrounding tissues. The frequency of MAGE-3 gene product expression was examined according to clinical stage and differentiation of histopathology. The results revealed no significant differences in MAGE gene product expression among the clinical stage and the grade of differentiation of histopathology according to Logistic Regression test(P>0.05).


CONCLUSIONS
The tumor-specific antigens might be used as molecular markers and targets for human renal and urinary bladder tumor.
Ma
Ming
M
Department of Urology, Peking University First Hospital, Beijing 100034, China.
Yu
Li-zhang
LZ
Na
Yan-qun
YQ
Geng
Lin
L
Li
Hong-wei
HW
Yang
Xin-yu
XY
Wang
Ying
Y
Ding
Yi
Y
Pan
Bo-nian
BN
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000032:analysis / Q000235:genetics*; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000737:chemistry / Q000235:genetics*; D008297:Male; D058950:Melanoma-Specific Antigens; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D001749:Urinary Bladder Neoplasms / Q000737:chemistry / Q000235:genetics*
D000951:Antigens, Neoplasm; C072011:MAGEA1 protein, human; C072013:MAGEA3 protein, human; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins; D012333:RNA, Messenger