Influence of clindamycin, metronidazole and polymyxin B on the expression of cell adhesion molecules stimulated by endotoxin and enterotoxin of Bacteroides fragilis.
The aim of this study was to compare the influence of antimicrobials (clindamycin, metronidazole and polymyxin B) on the expression of adhesion molecules (VCAM-1, ICAM-1 and E-selectin) on the HMEC-1 cell line stimulated by LPS and enterotoxin of B. fragilis. LPS was extracted from two reference: ATCC 43858 and NCTC 11295 and one isolated in our laboratory (W2) enterotoxigenic strains, and one nonenterotoxigenic reference strain--IPL E 323. Enterotoxin preparations (Tox 1 and Tox 2) were isolated from supematant of B. fragilis ATCC 43858 culture and purified. HMEC-1 cell line was stimulated with bacterial preparations at concentration of 10 mg/ml. For measuring the expression of adhesion molecules we used ELISA test. Clindamycin, metronidazole and polymyxin B supressed the ICAM-1 expression when endothelium was stimulated with B. fragilis LPS and augmented ICAM-1 expression by Tox 1 and Tox 2. The expression of VCAM-1 was augmented by antimicrobials when endothelium was stimulated with LPS or enterotoxin preparations. The expression of E-selectin was differentiated.
Meisel-Mikołajczyk
Felicja
F
Department of Medical Microbiology, Medical University of Warsaw, Warsaw, Poland. fmikolajczyk@post.pl
Rokosz
Alicja
A
Kot
Katarzyna
K
Zawidzka
Elwira
E
Malchar
Cezary
C
Nowaczyk
Maria
M
Górski
Andrzej
A
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001441:Bacteroides fragilis / Q000378:metabolism*; D002460:Cell Line; D002981:Clindamycin / Q000494:pharmacology; D019040:E-Selectin / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism* / Q000382:microbiology; D004731:Endotoxins / Q000494:pharmacology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D008666:Metalloendopeptidases / Q000494:pharmacology; D008795:Metronidazole / Q000494:pharmacology; D011112:Polymyxin B / Q000494:pharmacology; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis*
D000900:Anti-Bacterial Agents; D019040:E-Selectin; D004731:Endotoxins; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D008795:Metronidazole; D002981:Clindamycin; D008666:Metalloendopeptidases; C091198:fragilysin; D011112:Polymyxin B