Characteristics of calves produced with sperm sexed by flow cytometry/cell sorting.
The objectives of this study were to determine whether calves produced by sexed sperm differed from controls and to what extent the sex ratio of calves was altered by the sexing procedure. Data were collected from 1,169 calves produced from sperm sexed by flow cytometry/cell sorting after staining with Hoechst 33342, and 793 calves produced from control sperm during breeding trials between 1997 and 2001. Least squares ANOVA were completed using factors of treatment (sexed vs. control sperm), 19 management groups from 13 field trials, and calf sex. Responses analyzed include gestation length, birth weight, calving ease, calf vigor, weaning weight, abortion rate, and death rates (neonatal and through weaning). No significant difference was observed for any response due to treatment or treatment interactions (P > 0.10). Therefore, calves produced from sexed sperm grew and developed normally both pre- and postnatally. A neurological disorder was observed in four control calves and one sexed calf from one farm. No gross anatomical abnormalities were reported for any calves in the study. Differences were observed for all responses among management groups (P < 0.03 for abortions and P < 0.01 for all other responses). Heifer and bull calves differed (P < 0.001) in gestation length (278.4 and 279.6 d), birth weight (32.8 and 35.2 kg), calving ease (1.15 and 1.30), and weaning weight (233 and 247 kg). Gestation length did not affect characteristics of calves. The sex ratio at birth of calves from unsexed control sperm was 49.2% male. Sexing accuracy of X-sorted sperm was 87.8% female calves, and Y-sorted sperm produced 92.1% male calves. Flow cytometry/cell sorting can be used to preselect sex of calves safely with approximately 90% accuracy.
Tubman
L M
LM
Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins 80523-1683, USA.
Brink
Z
Z
Suh
T K
TK
Seidel
G E
GE
D000704:Analysis of Variance; D000818:Animals; D001724:Birth Weight; D001947:Breeding / Q000379:methods; D002417:Cattle / Q000502:physiology*; D002469:Cell Separation / Q000379:methods / Q000662:veterinary; D005314:Embryonic and Fetal Development; D005260:Female; D005434:Flow Cytometry / Q000379:methods / Q000662:veterinary*; D005865:Gestational Age; D008297:Male; D036801:Parturition / Q000502:physiology; D011247:Pregnancy; D011256:Pregnancy Outcome; D012680:Sensitivity and Specificity; D012743:Sex Preselection / Q000379:methods / Q000662:veterinary*; D013094:Spermatozoa / Q000166:cytology*; D014886:Weaning; D014960:X Chromosome; D014998:Y Chromosome