A tale of two integrations, transgene and T-DNA: gene targeting by homologous recombination in rice.
The first successful and reproducible gene targeting by homologous recombination, without the concomitant occurrence of ectopic events, has been reported. This will be a powerful approach for the characterization of gene function in rice, an important crop and a model for other cereal species. Models have been proposed to explain gene replacement by homologous recombination, including a possible model for Agrobacterium-mediated gene targeting using a strong positive-negative selection.
Iida
Shigeru
S
National Institute for Basic Biology, Okazaki 444-8585, Japan.
Terada
Rie
R
D004269:DNA, Bacterial; D018390:Gene Targeting; D012275:Oryza / Q000235:genetics*; D011995:Recombination, Genetic; D019076:Transgenes
D004269:DNA, Bacterial; C036483:T-DNA