Modulation of DNA repair and recombination by the bacteriophage lambda Orf function in Escherichia coli K-12.
The orf gene of bacteriophage lambda, fused to a promoter, was placed in the galK locus of Escherichia coli K-12. Orf was found to suppress the recombination deficiency and sensitivity to UV radiation of mutants, in a Delta(recC ptr recB recD)::P(tac) gam bet exo pae cI DeltarecG background, lacking recF, recO, recR, ruvAB, and ruvC functions. It also suppressed defects of these mutants in establishing replication of a pSC101-related plasmid. Compared to orf, the recA803 allele had only small effects on recF, recO, and recR mutant phenotypes and no effect on a ruvAB mutant. In a fully wild-type background with respect to known recombination and repair functions, orf partially suppressed the UV sensitivity of ruvAB and ruvC mutants.
Poteete
Anthony R
AR
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue, Worcester, MA 01655, USA. anthony.poteete@umassmed.edu
D000483:Alleles; D010582:Bacteriophage lambda / Q000235:genetics*; D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics*; D005814:Genes, Viral / Q000502:physiology*; D011995:Recombination, Genetic; D014466:Ultraviolet Rays