Resistance to imipenem, cefepime, and cefpirome associated with mutation in Omp36 osmoporin of Enterobacter aerogenes.
Enterobacter aerogenes develops increased multidrug resistance via a functional alteration of outer-membrane permeability associated with a decrease in porin function. We have sequenced the gene coding the major porin of Enterobacter aerogenes, omp36. The sequence shows a high similarity with the Klebsiella pneumoniae ompK36 gene and is closely related to the enterobacterial OmpC family. Sequence analysis of several Omp36 issued from clinical strains indicated variability in putative cell-surface exposed domains. Interestingly, substitution Gly112Asp was observed in the conserved eyelet L3 region of the porin produced by two strains, C and 3. This substitution is associated with a high general beta-lactam resistance observed in these isolates and with alteration of pore properties previously described in strain 3 porin [Mol. Microbiol. 41 (2001) 189]. This is the first genetic identification of impermeability-mediated resistance to beta-lactams in various clinical E. aerogenes strains.
Thiolas
Aurélie
A
Enveloppe Bactérienne, Perméabilité et Antibiotiques, EA2197, Faculté de Médecine, Université de la Méditerranée, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France.
Bornet
Charléric
C
Davin-Régli
Anne
A
Pagès
Jean-Marie
JM
Bollet
Claude
C
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D000077723:Cefepime; D002511:Cephalosporins / Q000494:pharmacology*; D017931:DNA Primers; D004352:Drug Resistance, Microbial / Q000235:genetics*; D021902:Enterobacter aerogenes / Q000187:drug effects* / Q000235:genetics; D015378:Imipenem / Q000494:pharmacology*; D009154:Mutation; D000097572:Cefpirome
D001425:Bacterial Outer Membrane Proteins; D000077723:Cefepime; D002511:Cephalosporins; D017931:DNA Primers; D015378:Imipenem; D000097572:Cefpirome