title: Oxidative stress and microglial activation in substantia nigra following striatal MPP+.
abstract: The present study aims to study sequential alterations occurring in both dopaminergic neurons and microglia in substantia nigra (SN) following intrastriatal injection of 1-methyl-4-phenylpridium ion (MPP+) in rats. Heme oxygenase-1 (HO-1), a marker of oxidative stress, first appeared in dopaminergic neurons in SN at 1 day post-lesion. Subsequently, microglia in SN exhibited morphological changes indicative of activation. At 7 days post-lesion, those findings increased severity and 7a significant reduction in the number of dopaminergic neurons was observed. The present finding suggests that extensive oxidative stress and secondary-induced neuroinflammation play a relevant role in MPP(+)-induced retrograde dopaminergic neuron degeneration. We hope that this model will be useful in developing a disease modifying therapy of Parkinson's disease.
authors.affiliation: Department of Neurology, Wakayama Medical University, 811-1 Kimiidera, Wakayama-city, Wakayama 641-8510, Japan. h-miwa@wakayama-med.ac.jph
authors.forename: Hideto
authors.initials: H
authors.lastname: Miwa
authors.forename: Tomomi
authors.initials: T
authors.lastname: Kubo
authors.forename: Shuhei
authors.initials: S
authors.lastname: Morita
authors.forename: Ichiro
authors.initials: I
authors.lastname: Nakanishi
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Kondo
mesh_terms: D015655:1-Methyl-4-phenylpyridinium / Q000633:toxicity*; D000818:Animals; D008297:Male; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013378:Substantia Nigra / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
chemical_list: D015655:1-Methyl-4-phenylpyridinium