Crystallization and preliminary X-ray diffraction analysis of the 16-haem cytochrome of Desulfovibrio gigas.
High-molecular-weight cytochromes (Hmcs) belong to a large family of multihaem cytochromes in sulfate-reducing bacteria. HmcA is the first cytochrome reported to have 16 c-type haems arranged in its polypeptide chain. The function of this cytochrome is still unknown, although it is clear that it belongs to a membrane-bound complex involved in electron transfer from the periplasm to the membrane. HmcA from Desulfovibrio gigas has been purified and successfully crystallized using the hanging-drop vapour-diffusion method. The crystals grew using PEG and zinc acetate as precipitants to maximum dimensions of 0.2 x 0.2 x 0.2 mm in an orthorhombic space group, with unit-cell parameters a = 88.9, b = 90.9, c = 83.7 A. The crystals diffracted to beyond 2.07 A and a MAD data set was collected.
Santos-Silva
Teresa
T
REQUIMTE/CQFB Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
Dias J
João M
JM
Bourenkov
Gleb
G
Bartunik
Hans
H
Moura
Isabel
I
Romão
Maria João
MJ
D003460:Crystallization; D018360:Crystallography, X-Ray; D045304:Cytochromes c / Q000737:chemistry* / Q000378:metabolism; D045286:Desulfovibrio gigas / Q000201:enzymology*; D016969:Desulfovibrio vulgaris / Q000201:enzymology; D006418:Heme / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D040681:Structural Homology, Protein
D006418:Heme; D045304:Cytochromes c