[Detection of low avidity IgG as a promising approach to diagnosis of primary herpetic infection].
A Russian immune-enzyme test-system ("HERPES-DIAGNOST") was designed on the basis of detection of low-avidity IgG antibodies in order to promote the laboratory value of serological examinations of patients with different clinical manifestations of the herpetic infection. The key test parameters were tuned; the immunosorbent production based on antigens (herpes simplex virus--HSV), types 1 and 2, was optimized; and the concentration was chosen for the main reagent that removes the low-avidity antibodies (8 M urea solution) and, finally, the temporal and temperature regimes were selected for testing. A system was elaborated for registering and interpreting the results. The avidity index of antibodies lower than 35% was found to be a reliable criterion confirming the presence of acute primary infection triggered both by HSV-1 and by HSV-2. If there is a relapse of herpetic infection, the avidity index of antibodies can range from 30 to 45%.
Ebralidze
L K
LK
Vedunova
S L
SL
Mal'tseva
N N
NN
Lavrov
V F
VF
Gol'tsov
V A
VA
Zverev
V V
VV
D000914:Antibodies, Viral / Q000097:blood*; D000915:Antibody Affinity; D000956:Antigens, Viral / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006561:Herpes Simplex / Q000097:blood / Q000175:diagnosis*; D018259:Herpesvirus 1, Human / Q000276:immunology* / Q000302:isolation & purification; D018258:Herpesvirus 2, Human / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013696:Temperature
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D011933:Reagent Kits, Diagnostic