title: Screening for antioxidant activity in edible plant products: comparison of low-density lipoprotein oxidation assay, DPPH radical scavenging assay, and Folin-Ciocalteu assay.
abstract: Oxidation of low-density lipoprotein (LDL) has been implicated in atherogenesis. Antioxidants that prevent LDL from oxidizing may reduce atherosclerosis. This study investigated LDL antioxidant activity in edible plant products for development of dietary supplementation to prevent atherosclerosis. Fifty-two kinds of edible plants were extracted using 70% aqueous ethanol solution, and the antioxidant activity of the extracts, which inhibit human LDL oxidation induced by copper ion, was determined on the basis of the oxidation lag time and represented as epigallocatechin 3-gallate equivalent. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and total phenolic content were also measured for comparisons with antioxidant activity in LDL. Plant products showing the greatest activity in LDL oxidation assay were akamegashiwa (Mallotus japonicus) leaf, Japanese privet (Ligustrum japonicum) leaf, green tea [Camellia sinensis (L.) O. Kuntze], and astringent persimmon (Diospyros kaki). The present study revealed high levels of LDL antioxidant activity in plant products for which such activity levels are underestimated in the DPPH radical scavenging assay and Folin-Ciocalteu assay.
authors.affiliation: Shimane Institute for Industrial Technology, 1 Hokuryo-cho, Matsue City, Shimane, 690-0816, Japan. katsube@hoho-shimane.or.jp
authors.forename: Takuya
authors.initials: T
authors.lastname: Katsube
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Tabata
authors.forename: Yukari
authors.initials: Y
authors.lastname: Ohta
authors.forename: Yukikazu
authors.initials: Y
authors.lastname: Yamasaki
authors.forename: Erdembileg
authors.initials: E
authors.lastname: Anuurad
authors.forename: Kuninori
authors.initials: K
authors.lastname: Shiwaku
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Yamane
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D001713:Biphenyl Compounds; D003300:Copper / Q000494:pharmacology; D016166:Free Radical Scavengers; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000737:chemistry; D008982:Molybdenum; D010636:Phenols / Q000032:analysis; D010851:Picrates; D010945:Plants, Edible / Q000737:chemistry*; D012680:Sensitivity and Specificity; D017973:Tungsten Compounds
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; C029556:Folin's phenol reagent; D016166:Free Radical Scavengers; D008077:Lipoproteins, LDL; D010636:Phenols; D010851:Picrates; D017973:Tungsten Compounds; D003300:Copper; D008982:Molybdenum; C004931:1,1-diphenyl-2-picrylhydrazyl