[A rapid procedure to purify serum IgG from Microtus fotis].
OBJECTIVE
To evaluate the procedure to purify IgG antibodies from Microtus fotis serum.


METHODS
IgG antibodies from sera of three groups of Microtus fotis were purified by protein G or protein A affinity chromatography, their purity and binding capacity were compared.


RESULTS
The protein G affinity chromatography was more efficient than protein A affinity chromatography. The antibodies isolated from protein G affinity chromatography showed a higher purity and better activity than that from protein A affinity chromatography monitored by SDS-PAGE and ELISA. The ability of the purified IgG to bind the second antibodies were 8.5 times and 3.1 times that of non-IgG proteins and unpurified sera, respectively.


CONCLUSIONS
The protein G affinity chromatography is a rapid, convenient and reliable procedure for Microtus fotis serum IgG purification.
Jiang
Shou-fu
SF
Shanghai Municipal Center for Disease Control & Prevention, Shanghai 200336.
Pan
Cai-e
CE
He
Yan-yan
YY
Zhu
Min
M
Li
Hao
H
Shi
Yao-jun
YJ
Wei
Mei-xiong
MX
D000818:Animals; D003411:Arvicolinae; D002846:Chromatography, Affinity / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood / Q000302:isolation & purification*; D051379:Mice; D008815:Mice, Inbred Strains; D009419:Nerve Tissue Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley; D013205:Staphylococcal Protein A
C029152:G-substrate; D007074:Immunoglobulin G; D009419:Nerve Tissue Proteins; D013205:Staphylococcal Protein A