Detection of large pathogenic expansions in FRDA1, SCA10, and SCA12 genes using a simple fluorescent repeat-primed PCR assay.
At least 18 human genetic diseases are caused by expansion of short tandem repeats. Here we describe a successful application of a fluorescent PCR method for the detection of expanded repeats in FRDA1, SCA10, and SCA12 genes. Although this test cannot give a precise estimate of the size of the expansion, it is robust, reliable, and inexpensive, and can be used to screen large series of patients. It proved useful for confirming the presence of large expansions in the Friedreich ataxia gene following an ambiguous result of long-range PCR, as well as rapid pre-screening for large repeat expansions associated with Friedreich ataxia and SCA10 and the shorter repeat expansions associated with SCA12.
Cagnoli
Claudia
C
Dipartimento di Genetica Biologia e Biochimica, Università degli Studi di Torino and Azienda Ospedaliera San Giovanni Battista di Torino, S.C. Genetica Medica, Torino, Italy.
Michielotto
Chiara
C
Matsuura
Tohru
T
Ashizawa
Tetsuo
T
Margolis
Russell L
RL
Holmes
Susan E
SE
Gellera
Cinzia
C
Migone
Nicola
N
Brusco
Alfredo
A
D016022:Case-Control Studies; D005453:Fluorescence; D005621:Friedreich Ataxia / Q000235:genetics* / Q000473:pathology; D006801:Humans; D033862:Iron-Binding Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020754:Spinocerebellar Ataxias / Q000235:genetics* / Q000473:pathology; D019680:Trinucleotide Repeat Expansion / Q000235:genetics*; D000096923:Frataxin
D033862:Iron-Binding Proteins; D000096923:Frataxin