title: Human papillomavirus frequency in oral epithelial lesions.
abstract: BACKGROUND
  Oral human papillomavirus (HPV) prevalence varies according to geographical occurrence, the type of lesion, and the method of diagnosis. The polymerase chain reaction method (PCR) appears to be more sensitive and can be easily applicable to epidemiologic studies.
  
  
  OBJECTIVE
  To determine the frequency of HPV and its genotypes in oral lesions among patients attending a reference clinic of a university hospital.
  
  
  METHODS
  PCR was performed to identify HPV DNA from samples of oral epithelial lesions in 80 patients. For HPV DNA amplification, MY09/MY11 consensus primers were used and specific genotypes were identified through restriction fragment of length polymorphism (RFLP) pattern.
  
  
  RESULTS
  HPV DNA was present in 11.3% of patients, and the identified genotypes were 6b, MM4 (W13B), and MM9 (PAP238A).
  
  
  CONCLUSIONS
  HPV DNA frequency in patients with oral epithelial lesions was 11.3%. The genotypes MM4 and MM9 are uncommon in oral lesions, and they are characterized as high-risk HPV types in those types of lesions.
authors.affiliation: Federal University of Rio Grande do Sul, Porto Alegre, Brazel. carlaz44@terra.com.br
authors.forename: Carmen Maria
authors.initials: CM
authors.lastname: Lazzari
authors.forename: Luciano Percival
authors.initials: LP
authors.lastname: Krug
authors.forename: Onofre Francisco
authors.initials: OF
authors.lastname: Quadros
authors.forename: Cristiano Bozzetti
authors.initials: CB
authors.lastname: Baldi
authors.forename: Mary Clarisse
authors.initials: MC
authors.lastname: Bozzetti
mesh_terms: D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000032:analysis; D004848:Epithelium / Q000821:virology; D006801:Humans; D008875:Middle Aged; D009059:Mouth Diseases / Q000821:virology*; D009061:Mouth Mucosa / Q000821:virology; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D030361:Papillomavirus Infections / Q000821:virology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004279:DNA, Viral