title: A new hybrid rescue allele in Drosophila melanogaster.
abstract: Crosses of Drosophila melanogaster females to males of its sibling species Drosophila simulans, Drosophila mauritiana and Drosophila sechellia produce no sons and daughters that are viable only at low temperatures. We describe here a novel rescue allele Df(1)EP307-1-2 isolated on the basis of its suppression of high temperature hybrid female lethality. Df(1)EP307-1-2 also rescues hybrid males to the pharate adult stage, the same stage at which it is lethal to D. melanogaster pure species males. Molecular analysis indicates that Df(1)EP307-1-2 is associated with a deletion of about 61 kb in the 9D region of the X chromosome. The structure of Df(1)EP307-1-2 suggests that it was formed by a process similar to P-element induced male recombination.
authors.affiliation: Department of Genetics, University of Cambridge, Cambridge, UK. dabarbash@ucdavis.edu
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Barbash
authors.forename: John
authors.initials: J
authors.lastname: Roote
authors.forename: Glynnis
authors.initials: G
authors.lastname: Johnson
authors.forename: Michael
authors.initials: M
authors.lastname: Ashburner
mesh_terms: D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D017931:DNA Primers; D004330:Drosophila / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D017353:Gene Deletion; D005816:Genetic Complementation Test; D008297:Male; D008957:Models, Genetic; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D012737:Sex Factors; D013696:Temperature; D014960:X Chromosome
chemical_list: D017931:DNA Primers