Differential effects of isoflavones and lignans on invasiveness of MDA-MB-231 breast cancer cells in vitro.
Metastasis is a major cause of morbidity and mortality in breast cancer with tumour cell invasion playing a crucial role in the metastatic process. The effects of a panel of phytoestrogens, including isoflavones and lignans on the invasion of a breast cancer cell-line (MDA-MB-231) through Matrigel were assessed. Genistein, glycitein, daidzein, equol, O-Desmethylangolensin (O-Dma) and coumestrol exerted a potent inhibitory effect on cell invasion (e.g. inhibition of 41.7+/-15% (P = 0.007) coumestrol (10 microM)). In contrast the lignans exerted minimal effects on invasion. Inhibition of invasion induced by the phytoestrogens occurred without affecting cell viability, highlighting the possible chemoprotective effects of these compounds.
Magee
Pamela J
PJ
Northern Ireland Centre for Food and Health (NICHE), School of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, Northern Ireland, UK. pj.magee@ulster.ac.uk
McGlynn
Hugh
H
Rowland
Ian R
IR
D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D004338:Drug Combinations; D005260:Female; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D007797:Laminin / Q000378:metabolism; D017705:Lignans / Q000494:pharmacology*; D009361:Neoplasm Invasiveness / Q000517:prevention & control; D011509:Proteoglycans / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004338:Drug Combinations; D007529:Isoflavones; D007797:Laminin; D017705:Lignans; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen