title: Cytogenetic analysis using fluorescence in situ hybridization (FISH) to evaluate occupational exposure to carcinogens.
abstract: Chromosomal aberrations determined by conventional method or fluorescence in situ hybridization (FISH) technique with whole chromosome painting are used as biomarkers of effect. Groups occupationally exposed to 1,3-butadiene (BD), acrylonitrile, ethyl benzene and benzene in petrochemical industry, and carcinogenic polycyclic aromatic hydrocarbons (c-PAHs) from ambient air were followed by conventional method and FISH painting for chromosomes # 1 and # 4, in total 383 subjects, including controls. No effect was observed by either method with exposure to 1,3-butadiene < 1mg/m(3) and acrylonitrile < 0.3mg/m(3). Ethyl benzene and benzene exposure significantly increased chromosomal aberrations by both methods, which decreased after the implementation of preventive measures. The genomic frequency of translocations by FISH calculated as FG/100 was significantly increased in city policemen versus control group exposed to c-PAHs from ambient air (1.72+/-1.57 versus 1.25+/-1.11, P<0.05). The method of FISH with whole chromosome painting seems to be more sensitive to detect chromosomal injury by occupational exposure to carcinogens than conventional method.
authors.affiliation: Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine AS CR and Health Institute of Central Bohemia, 142 20 Prague 4, Czech Republic. sram@biomed.cas.cz
authors.forename: Radim J
authors.initials: RJ
authors.lastname: Sram
authors.forename: Olena
authors.initials: O
authors.lastname: Beskid
authors.forename: Blanka
authors.initials: B
authors.lastname: Binkova
authors.forename: Pavel
authors.initials: P
authors.lastname: Rossner
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Smerhovsky
mesh_terms: D000181:Acrylonitrile / Q000032:analysis / Q000633:toxicity; D000328:Adult; D001555:Benzene Derivatives / Q000032:analysis / Q000633:toxicity; D002070:Butadienes / Q000032:analysis / Q000633:toxicity; D002273:Carcinogens / Q000032:analysis*; D002617:Chemical Industry; D002869:Chromosome Aberrations / Q000139:chemically induced; D020223:Chromosome Painting; D020732:Cytogenetic Analysis; D005260:Female; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects*; D016495:Police; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity
chemical_list: D001555:Benzene Derivatives; D002070:Butadienes; D002273:Carcinogens; D005819:Genetic Markers; D011084:Polycyclic Aromatic Hydrocarbons; C031763:1,3-butadiene; C004912:ethylbenzene; D000181:Acrylonitrile