Transgene integration in plants: poking or patching holes in promiscuous genomes?
Transgene integration in plants transformed by either Agrobacterium or direct DNA delivery methods occurs through illegitimate recombination (IR). The precise mechanism(s) for IR-mediated transgene integration and the role of host double-strand break repair enzymes remain unknown. A recent wealth of sequenced transgene loci and investigations aimed at genetically dissecting transgene integration mechanism(s) have provided new insights into the process.
Somers
David A
DA
Department of Agronomy and Plant Genetics, Plant Molecular Genetics Institute, University of Minnesota, 1991 Upper Buford Circle, St. Paul, Minnesota, USA. somers@biosci.cbs.umn.edu
Makarevitch
Irina
I
D004247:DNA / Q000235:genetics / Q000378:metabolism; D004260:DNA Repair; D004269:DNA, Bacterial; D018390:Gene Targeting / Q000379:methods; D018014:Gene Transfer Techniques; D018745:Genome, Plant; D030821:Plants, Genetically Modified; D019076:Transgenes
D004269:DNA, Bacterial; C036483:T-DNA; D004247:DNA