title: Normal skin of HIV-infected individuals contains increased numbers of dermal CD8 T cells and normal numbers of Langerhans cells.
abstract: Dysregulation of the skin immune system (SIS) could explain the high prevalence of skin disorders in HIV+ individuals. The present study was carried out to determine whether alterations in the cell population of SIS and epidermal immunoactivation occur in the normal skin of HIV+ individuals. Forty-five biopsies were taken from the normal upper arm skin of 45 HIV+ patients and of 15 healthy controls. HIV+ individuals were divided into three categories according to their CD4 cell blood count (<200, 200-499 and > or = 500/microl). Hematoxylin-eosin was used to stain tissue sections for morphological analysis and immunohistochemistry was used for the evaluation of the frequency of macrophages, Langerhans cells, and CD lymphocyte subsets. In addition, semiquantitative analysis of LFA-1, ICAM-1 and HLA-DR was determined in epidermal cells. Macrophages, Langerhans cells, and CD lymphocyte subsets did not differ significantly between any of the patient categories and the control group. When all HIV+ individuals were compared as a group to the control group, a significant increase in dermal CD8+ T lymphocytes (P < 0.01) and lower CD4-CD8 ratios (P < 0.01) were observed in the HIV+ individuals. Epidermal ICAM-1 and HLA-DR expression was negative in both HIV+ and normal skin biopsies. No evidence of a depletion of the SIS population or of epidermal immunoactivation in normal skin from HIV+ individuals was demonstrable, suggesting that alterations in the central immune system are not necessarily reflected in the SIS of HIV-infected patients.
authors.affiliation: Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil. mclara@ipec.fiocruz.br
authors.forename: M C G
authors.initials: MC
authors.lastname: Galhardo
authors.forename: F F
authors.initials: FF
authors.lastname: Alvarenga
authors.forename: G
authors.initials: G
authors.lastname: Schueler
authors.forename: M
authors.initials: M
authors.lastname: Perez
authors.forename: M G
authors.initials: MG
authors.lastname: Morgado
authors.forename: H
authors.initials: H
authors.lastname: Ferreira
authors.forename: L M S
authors.initials: LM
authors.lastname: Azevedo
authors.forename: E P
authors.initials: EP
authors.lastname: Sampaio
authors.forename: E N
authors.initials: EN
authors.lastname: Sarno
mesh_terms: D000328:Adult; D001706:Biopsy; D016516:CD4-CD8 Ratio; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D016022:Case-Control Studies; D005260:Female; D015658:HIV Infections / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D007801:Langerhans Cells / Q000276:immunology / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D012867:Skin / Q000276:immunology / Q000473:pathology*