title: Detection of pepsin in tracheal secretions after forced small-volume aspirations of gastric juice.
abstract: BACKGROUND
  Detecting small-volume aspirations of gastric contents is an important but difficult task. A potentially useful method for this purpose is assaying tracheal secretions for pepsin, an expected constituent of gastric juice.
  
  
  METHODS
  A 2-group experimental design was used. The primary subjects were 161 experimental and 21 control New Zealand white rabbits; 161 acutely ill humans provided the gastric juice used in the project. The animals were anesthetized before being intubated and mechanically ventilated. Three separate boluses of human gastric juice mixed with dye-stained enteral formula were instilled into the experimental animals' tracheas; the 21 control animals received only 0.9% sodium chloride solution. At the beginning of each experiment, 0.4 mL/kg of the substance was infused over a 30-minute period; the infusion was then stopped and 90 minutes were allowed to elapse before endotracheal suctioning was performed. This procedure was repeated at hour 2 and hour 4. After completion of the multiple aspiration portion of the study, 23 additional animals were subjected to a single aspiration of 0.4 mL/kg of a mixture of human gastric juice and dye-stained enteral formula; secretions were obtained at 2 hours, 4 hours, and 6 hours. An immunoassay was used to test for pepsin in all of the tracheal secretions.
  
  
  RESULTS
  In the 3-aspiration group, pepsin was found in all of the secretions from 92.5% (149/161) of the experimental animals; in contrast, no pepsin was found in any of the secretions from the 20 control animals. In the single-aspiration group, pepsin was found in all of the tracheal secretions from the 23 animals at 2 hours and 4 hours and 21 of the 23 animals at 6 hours.
  
  
  CONCLUSIONS
  The immunoassay used in this animal model study was able to detect pepsin in >90% of the experimental animals' tracheal secretions after multiple or single forced aspirations of gastric juice. The extent to which pepsin can be detected in the tracheal secretions of acutely ill tube-fed humans requires investigation, as does the extent to which clinical outcomes are affected by pepsin-positive tracheal secretions.
authors.affiliation: St. Louis University, St. Louis, Missouri 63104, USA. methenna@slu.edu
authors.forename: Norma A
authors.initials: NA
authors.lastname: Metheny
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Dahms
authors.forename: Yie-Hwa
authors.initials: YH
authors.lastname: Chang
authors.forename: Barbara J
authors.initials: BJ
authors.lastname: Stewart
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Frank
authors.forename: Ray E
authors.initials: RE
authors.lastname: Clouse
mesh_terms: D000818:Animals; D004396:Coloring Agents; D016638:Critical Illness; D004195:Disease Models, Animal; D004750:Enteral Nutrition; D005750:Gastric Juice; D006801:Humans; D010434:Pepsin A / Q000302:isolation & purification*; D011015:Pneumonia, Aspiration / Q000175:diagnosis*; D011817:Rabbits; D011897:Random Allocation; D012680:Sensitivity and Specificity; D014132:Trachea / Q000737:chemistry*
chemical_list: D004396:Coloring Agents; D010434:Pepsin A