title: Substance P axons and sensory threshold increase in burn-graft human skin.
abstract: BACKGROUND
  Our knowledge of afferent nerve fiber reinnervation of grafted skin following third-degree burn is limited by a lack of quantitative histological and psychophysical assessment from the same cutaneous area. The current study compares fiber profile and functional recovery measurements in injured and control skin from the same subject.
  
  
  METHODS
  Nerve regeneration and modality-specific sensory thresholds were compared using immunocytochemical labeling with protein gene product 9.5 antibody to stain all axons and anti-substance P to label substance P axons (which are predominantly unmyelinated), as well as computerized instrumentation to obtain psychophysical estimates.
  
  
  RESULTS
  Compared to control skin, threshold measures of pinprick (P < 0.001), warming (P < 0.001), touch (P < 0.001), and vibration (P < 0.01) were significantly elevated in burn-graft skin and correlated with histological analysis of skin biopsies obtained from the same site. Immunohistochemical staining of all axons innervating the dermis and epidermis revealed a significant reduction in burn-graft relative to control skin (54% decrease, P < 0.0001). In contrast, the incidence of substance P nerve fibers was significantly elevated in burn-graft (177% increase, P < 0.05) and appeared to correlate with patient reports of pruritus and pain.
  
  
  CONCLUSIONS
  Observations support the hypothesis that sensory regeneration is fiber-size-dependent in burn-graft skin. The findings that substance P fiber growth increased while total fiber count decreased and that thermal threshold showed the greatest degree of functional recovery suggest that unmyelinated neurons have the greater ability to transverse scar tissue and reinnervate grafted skin following third-degree burn injury.
authors.affiliation: Department of Physical Therapy, University of Utah, Salt Lake City, Utah, USA.
authors.forename: R Scott
authors.initials: RS
authors.lastname: Ward
authors.forename: Robert P
authors.initials: RP
authors.lastname: Tuckett
authors.forename: Kathleen B
authors.initials: KB
authors.lastname: English
authors.forename: Olle
authors.initials: O
authors.lastname: Johansson
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Saffle
mesh_terms: D000328:Adult; D001369:Axons / Q000502:physiology*; D002056:Burns / Q000503:physiopathology*; D062109:Dermatologic Surgical Procedures; D006801:Humans; D008875:Middle Aged; D009416:Nerve Regeneration; D009619:Nociceptors / Q000502:physiology; D010146:Pain / Q000503:physiopathology; D011537:Pruritus / Q000503:physiopathology; D020127:Recovery of Function; D012684:Sensory Thresholds; D012867:Skin / Q000294:innervation*; D016038:Skin Transplantation; D013373:Substance P / Q000502:physiology*; D014110:Touch; D014732:Vibration
chemical_list: D013373:Substance P