Rapid colorimetric assay for antimicrobial susceptibility testing of Pseudomonas aeruginosa.
A colorimetric assay based on the reduction of a tetrazolium salt [2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT)] for rapidly determining the susceptibility of Pseudomonas aeruginosa isolates to bactericidal antibiotics is described. There was excellent agreement between the tobramycin and ofloxacin MICs determined after 5 h using the XTT assay and after 18 h using conventional methods. The data suggests that an XTT-based assay could provide a useful method for rapidly determining the susceptibility of P. aeruginosa to bactericidal antibiotics.
Tunney
Michael M
MM
The Clinical and Practice Research Group, School of Pharmacy, The Queen's University of Belfast, Belfast BT9 7BL, United Kingdom. m.tunney@qub.ac.uk
Ramage
Gordon
G
Field
Tyler R
TR
Moriarty
Thomas F
TF
Storey
Douglas G
DG
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology; D015169:Colony Count, Microbial; D003124:Colorimetry; D008826:Microbial Sensitivity Tests; D015242:Ofloxacin / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D015203:Reproducibility of Results; D013778:Tetrazolium Salts; D014031:Tobramycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D013778:Tetrazolium Salts; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide; D015242:Ofloxacin; D014031:Tobramycin