title: Natural polymorphism of cytomegalovirus DNA polymerase lies in two nonconserved regions located between domains delta-C and II and between domains III and I.
abstract: We described the natural polymorphism of cytomegalovirus DNA polymerase in 42 unrelated isolates susceptible to ganciclovir, foscarnet, and cidofovir. All variations, including an eight-amino-acid deletion, were located between domains delta-C and II and between domains III and I, suggesting that these specific residues are not involved in enzymatic functions.
authors.affiliation: Virology Laboratories, Hospital AP-HP Pitié-Salpêtrière and University Paris VI, Paris, France. anne-marie.fillet@psl.ap-hop-paris.fr
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Fillet
authors.forename: Laetitia
authors.initials: L
authors.lastname: Auray
authors.forename: Sophie
authors.initials: S
authors.lastname: Alain
authors.forename: Karine
authors.initials: K
authors.lastname: Gourlain
authors.forename: Berthe Marie
authors.initials: BM
authors.lastname: Imbert
authors.forename: Fatiha
authors.initials: F
authors.lastname: Najioullah
authors.forename: Gael
authors.initials: G
authors.lastname: Champier
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Gouarin
authors.forename: Jocelyne
authors.initials: J
authors.lastname: Carquin
authors.forename: Nadhira
authors.initials: N
authors.lastname: Houhou
authors.forename: Isabelle
authors.initials: I
authors.lastname: Garrigue
authors.forename: Alexandra
authors.initials: A
authors.lastname: Ducancelle
authors.forename: Danielle
authors.initials: D
authors.lastname: Thouvenot
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Mazeron
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000998:Antiviral Agents / Q000494:pharmacology; D017124:Conserved Sequence; D003587:Cytomegalovirus / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D017353:Gene Deletion; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000998:Antiviral Agents; D004259:DNA-Directed DNA Polymerase