title: Crystallization and preliminary crystallographic analysis of a native chitinase from the fungal pathogen Aspergillus fumigatus YJ-407.
abstract: Chitinase hydrolyzes chitin, a linear polymer of beta-1,4-linked N-acetylglucosamine (NAG), and plays a variety of roles in the biological world. In addition to endo- and exo-hydrolytic activities, transglycosyl activity has also been observed in the extracellular chitinase (afCHI) from the airborne saprophytic fungi Aspergillus fumigatus YJ-407. Crystals of this native chitinase have been grown at 291 K using PEG 3350 as a precipitant. The diffraction data from the crystal extend to 1.7 A resolution at BSRF, China. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 95.7, b = 100.5, c = 134.3 A. The presence of two molecules per asymmetric unit gives a crystal volume per protein mass (V(M)) of 3.6 A(3) Da(-1) and a solvent content of 65% by volume. A full set of X-ray diffraction data was collected to 2.1 A resolution.
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Hu
authors.forename: Ganggang
authors.initials: G
authors.lastname: Wang
authors.forename: Haitao
authors.initials: H
authors.lastname: Yang
authors.forename: Ju
authors.initials: J
authors.lastname: Zhou
authors.forename: Lijuan
authors.initials: L
authors.lastname: Mo
authors.forename: Kailin
authors.initials: K
authors.lastname: Yang
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Jin
authors.forename: Cheng
authors.initials: C
authors.lastname: Jin
authors.forename: Zihe
authors.initials: Z
authors.lastname: Rao
mesh_terms: D001232:Aspergillus fumigatus / Q000201:enzymology*; D002688:Chitinases / Q000737:chemistry*; D018360:Crystallography, X-Ray; D011487:Protein Conformation
chemical_list: D002688:Chitinases