Molecular cytogenetic definition of the chicken genome: the first complete avian karyotype.
Chicken genome mapping is important for a range of scientific disciplines. The ability to distinguish chromosomes of the chicken and other birds is thus a priority. Here we describe the molecular cytogenetic characterization of each chicken chromosome using chromosome painting and mapping of individual clones by FISH. Where possible, we have assigned the chromosomes to known linkage groups. We propose, on the basis of size, that the NOR chromosome is approximately the size of chromosome 22; however, we suggest that its original assignment of 16 should be retained. We also suggest a definitive chromosome classification system and propose that the probes developed here will find wide utility in the fields of developmental biology, DT40 studies, agriculture, vertebrate genome organization, and comparative mapping of avian species.
Masabanda
Julio S
JS
Cell and Chromosome Biology Group, Department of Biological Sciences, Brunel University, Uxbridge, Middlesex UB8 3PH, United Kingdom.
Burt
Dave W
DW
O'Brien
Patricia C M
PC
Vignal
Alain
A
Fillon
Valerie
V
Walsh
Philippa S
PS
Cox
Helen
H
Tempest
Helen G
HG
Smith
Jacqueline
J
Habermann
Felix
F
Schmid
Michael
M
Matsuda
Yoichi
Y
Ferguson-Smith
Malcolm A
MA
Crooijmans
Richard P M A
RP
Groenen
Martien A M
MA
Griffin
Darren K
DK
D000818:Animals; D002478:Cells, Cultured; D002645:Chickens / Q000235:genetics*; D002874:Chromosome Mapping; D020223:Chromosome Painting; D002875:Chromosomes / Q000145:classification; D002999:Clone Cells; D020732:Cytogenetic Analysis; D005347:Fibroblasts / Q000166:cytology; D008040:Genetic Linkage; D016678:Genome; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D009697:Nucleolus Organizer Region