Metabolic fingerprinting of wild type and transgenic tobacco plants by 1H NMR and multivariate analysis technique.
The metabolomic analysis of wild type and constitutive salicylic acid producing tobacco plants (CSA tobacco, Nicotiana tabacum 'Samsun' NN) plants overexpressing salicylate biosynthetic genes was carried out by 1H NMR spectrometry and multivariate analysis techniques. The principle component analysis (PCA) of the 1H NMR spectra showed a clear discrimination between those samples by PC1 and PC2. The discrimination of non-inoculated, TMV-virus inoculated, and systemic leaves or veins could also be obtained by PCA analysis. Major peaks in 1H NMR spectra contributing to the discrimination were assigned as those of chlorogenic acid, malic acid, and sugars. This method allows an efficient differentiation between wild type and transgenic plants without any pre-purification steps.
Choi
Hyung-Kyoon
HK
Department of Pharmacognosy, Section of Metabolomics, Institute of Biology, Leiden University, PO Box 9502, 2300 RA Leiden, The Netherlands.
Choi
Young Hae
YH
Verberne
Marianne
M
Lefeber
Alfons W M
AW
Erkelens
Cornelis
C
Verpoorte
Robert
R
D002726:Chlorogenic Acid / Q000378:metabolism / Q000494:pharmacology; D005960:Glucosides / Q000378:metabolism; D008293:Malates / Q000378:metabolism / Q000494:pharmacology; D015999:Multivariate Analysis; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D010936:Plant Extracts / Q000378:metabolism; D018515:Plant Leaves / Q000033:anatomy & histology / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D020156:Salicylic Acid / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000472:pathogenicity
D005960:Glucosides; D008293:Malates; D010936:Plant Extracts; D002726:Chlorogenic Acid; C030298:malic acid; D020156:Salicylic Acid