Two-photon imaging of calcium accumulation in rat cerebellar granule cells.
Topical accumulation of calcium ions in neurites and cell bodies of rat cerebellar granule cells was studied by two-photon microscopy in neurons loaded with the Ca-sensitive fluorescent indicator Oregon Green 488 Bapta. High potassium caused a rapid surge of internal calcium ([Ca2+]i) in the cell body, followed by a plateau. In neurites, [Ca2+]i reached a peak and then decreased back to the control level. In contrast, in neurons stimulated by NMDA, [Ca2+]i reached a steady level and remained constant as long as the agonist was present in the bath, either in the cell bodies or in neurites. In the latter, the response to NMDA treatment was smaller and heterogeneous, and [Ca2+]i increased in certain segments of the neurite, but not in others.
Pellistri
Francesca
F
INFM, Dipartimento di Fisica, Università di Genova, Via Dodecaneso 33, 16146 Genova, Italy.
Cupello
Aroldo
A
Esposito
Alessandro
A
Marchetti
Carla
C
Robello
Mauro
M
D000818:Animals; D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D002531:Cerebellum / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D016202:N-Methylaspartate / Q000494:pharmacology; D009474:Neurons / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D016202:N-Methylaspartate; D011189:Potassium Chloride; D002118:Calcium