title: Analysis of sugar epimers using mass spectrometry: N-acetyllactosamine-6,6'-disulfate and the 2'-epimer.
abstract: Galbeta1-4GlcNAc-6,6'-disulfate and 2'-epimer corresponding to Galbeta1- 4ManNAc-6,6'-disulfate were distinguished by mass spectrometry by utilizing fast atom bombardment (FAB), electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) methods. As for the steric information, negative-ion ESI mass spectrometry/mass spectrometry (MS/MS) provides the most extensive data, but FAB MS/MS also reveals detailed structural information of interest in our case, where MALDI MS is not yet fully equipped with post-source decay.
authors.affiliation: Center for Instrumental Analysis, The University of Electro-Communications, 1-5-1 Chofugaoka, Chofu, Tokyo 182-8585, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ohashi
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Itoh
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kubota
authors.forename: Kensaku
authors.initials: K
authors.lastname: Hamada
authors.forename: Mamoru
authors.initials: M
authors.lastname: Ohashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Hirano
authors.forename: Haruki
authors.initials: H
authors.lastname: Niwa
mesh_terms: D000606:Amino Sugars / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D013058:Mass Spectrometry; D008958:Models, Molecular; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000606:Amino Sugars; C487624:N-acetyllactosamine-6,6'-disulfate; C000458:N-acetyllactosamine