title: Virulence properties of rough and smooth strains of Vibrio cholerae O1.
abstract: A comparative study was carried out to see the differences in pathogenicity of rough and smooth strains. A total of 10 strains including 5 each of rough and smooth strains of Vibrio cholerae O1 were tested and found positive for toxin production by enzyme-linked immunosorbent assay (ELISA) in Richardson's and AKI media. All the smooth and rough strains, except one, showed a titre of 1: 10 and 1: 100 in Richardson's and AKI media, respectively. Both types of strains produced enterotoxin in rabbit ileal loop (RIL). The differences in multiplication abilities of smooth and rough strains in RIL were statistically significant (P <0.05). However, these differences in multiplying abilities did not influence the adherence potential or enterotoxin production as there was no significant difference (P >0.05) between these properties. This study demonstrated that the rough strains are equally pathogenic and as important as smooth strains.
authors.affiliation: International Centre for Diarrhoeal Disease Research, Bangladesh. sislam@icddrb.org
authors.forename: Mohammad Sirajul
authors.initials: MS
authors.lastname: Islam
authors.forename: Sunjukta
authors.initials: S
authors.lastname: Ahsan
authors.forename: Sirajul Islam
authors.initials: SI
authors.lastname: Khan
authors.forename: Qazi Shafi
authors.initials: QS
authors.lastname: Ahmed
authors.forename: Mohammad Harunur
authors.initials: MH
authors.lastname: Rashid
authors.forename: Khan M Nasirul
authors.initials: KM
authors.lastname: Islam
authors.forename: Richard Bradley
authors.initials: RB
authors.lastname: Sack
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D002772:Cholera Toxin / Q000096:biosynthesis; D003470:Culture Media; D006801:Humans; D007082:Ileum / Q000382:microbiology / Q000503:physiopathology; D010641:Phenotype; D011817:Rabbits; D041241:Vibrio cholerae O1 / Q000472:pathogenicity* / Q000502:physiology*; D014774:Virulence
chemical_list: D003470:Culture Media; D002772:Cholera Toxin