Chemopreventive effects of hydroxymatairesinol on uterine carcinogenesis in Donryu rats.
Hydroxymatairesinol (HMR), obtained from the heartwood of spruce (Picea abies), has been demonstrated to exert chemo-preventive effects on the development of mammary tumors in rats. To examine the influence of HMR on uterine carcinogenesis, adult Donryu rats were initiated with a single intrauterine treatment of N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG) at 11 weeks of age and fed thereafter 0, 200, or 600 ppm HMR mixed in the soy-containing diet until 15 months of age. Incidences of uterine adenocarcinoma in both 200 and 600 ppm HMR-dosed groups were significantly reduced to 11% and 15%, respectively, less than 50% of 0 ppm, at the end of the experiment (P < 0.05). A delay in the start of persistent estrus by HMR was observed at 8 months of age compared with controls given carcinogen alone. From urinalysis, HMR was metabolized mainly to enterolactone and hydroxyenterolactone. These findings suggest that HMR or its metabolites exert chemo-preventive effects in the rat ENNG-uterine carcinogenesis model.
Katsuda
Shin-ichi
S
Department of Biological Safety Research, Japan Food Research Laboratories, Tama-shi, Tokyo 206-0025, Japan. katudas@jfrl.or.jp
Yoshida
Midori
M
Saarinen
Niina
N
Smeds
Annika
A
Nakae
Dai
D
Santti
Risto
R
Maekawa
Akihiko
A
D000230:Adenocarcinoma / Q000139:chemically induced / Q000517:prevention & control*; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002273:Carcinogens / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004971:Estrus; D005247:Feeding Behavior; D005260:Female; D017705:Lignans / Q000494:pharmacology*; D008769:Methylnitronitrosoguanidine / Q000031:analogs & derivatives* / Q000633:toxicity; D051381:Rats; D014594:Uterine Neoplasms / Q000139:chemically induced / Q000517:prevention & control*
D016588:Anticarcinogenic Agents; D002273:Carcinogens; D017705:Lignans; C419306:hydroxymatairesinol; D008769:Methylnitronitrosoguanidine; C013525:ENNG