title: Detecting and quantifying apoptosis in tissue sections.
abstract: Various techniques exist for the identification of apoptosis in tissue sections or intact cells. The use of simple morphology, electron microscopy, DNA-end labeling techniques, and immunochemical methods are reviewed, with a particular emphasis on in situ end-labeling. The analysis of apoptotic cells and methods for their quantification are also discussed.
authors.affiliation: Department of Pathology, Addenbrookes Hospital, Cambridge University, UK.
authors.forename: Vicki
authors.initials: V
authors.lastname: Save
authors.forename: Peter A
authors.initials: PA
authors.lastname: Hall
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Coates
mesh_terms: D017209:Apoptosis; D004247:DNA / Q000378:metabolism; D053938:DNA Fragmentation; D007150:Immunohistochemistry / Q000379:methods*; D020287:In Situ Nick-End Labeling / Q000379:methods
chemical_list: D004247:DNA