Novel inhalational murine model of invasive pulmonary aspergillosis.
We developed a novel model of invasive aspergillosis (IA) that recapitulates human disease. Mice were immunosuppressed with cyclophosphamide and cortisone acetate and then infected in an aerosol chamber. This procedure reproducibly delivered 1 x 10(3) to 3 x 10(3) conidia to the lungs. Lethal pulmonary IA developed over 2 weeks and was prevented by amphotericin B.
Sheppard
Donald C
DC
St. John's Cardiovascular Research Center, Division of Infectious Diseases, Department of Medicine, Harbor-UCLA Research and Education Institute, Torrance, California 90502, USA.
Rieg
Gunter
G
Chiang
Lisa Y
LY
Filler
Scott G
SG
Edwards
John E
JE
Ibrahim
Ashraf S
AS
D000666:Amphotericin B / Q000627:therapeutic use; D000818:Animals; D000935:Antifungal Agents / Q000627:therapeutic use; D001229:Aspergillosis, Allergic Bronchopulmonary / Q000382:microbiology* / Q000473:pathology / Q000517:prevention & control; D001273:Atmosphere Exposure Chambers; D003348:Cortisone / Q000494:pharmacology; D003365:Costs and Cost Analysis; D003520:Cyclophosphamide / Q000494:pharmacology; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D007166:Immunosuppressive Agents / Q000494:pharmacology; D019570:Inhalation Exposure; D007970:Leukopenia / Q000139:chemically induced / Q000150:complications; D008168:Lung / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D011650:Pulmonary Alveoli / Q000473:pathology; D015203:Reproducibility of Results; D016019:Survival Analysis
D000935:Antifungal Agents; D007166:Immunosuppressive Agents; D000666:Amphotericin B; D003520:Cyclophosphamide; D003348:Cortisone