[Synergetic germicidal effect of ultrasonic with glutaraldehyde and its application in clinical disinfection].
OBJECTIVE
To study the synergetic germicidal effect of ulstrasonic with glutaraldehyde and the feasibility of combining ulstrasonic and glutaraldehyde to disinfect endocopes and medical instruments.


METHODS
We determined the respective and the synergetic killing rates of Bacillus subtilis var. niger of ulstrasonic and glutaraldehyde using quantitative suspenion germicidal tests and simulated on-the-spot tests.


RESULTS
In suspenion germicidal tests, because of the synergetic germicidal effect, the concentration of glutaraldehyde needed for sterilize spores was decreased from 20 mg/ml to 5 mg/ml for the same killing time(4 h), and when the concentrations were still 20 mg/ml, its killing time was shorten from 4 h to 1 h. Further more, the solutions containing 5 mg/ml glutaraldehyde could sterilize spores of Bacillus subtilis var. niger in 2 h. In the simulated on-the-spot tests, the concentration of glutaraldehyde needed for sterilization was decreased from 20 mg/ml to 5 mg/ml and its killing time was shorten from 4 h to 1 h under the synergetic germicidal effect.


CONCLUSIONS
It indicats the feasibility of combining ulstrasonic and glutaraldehyde to disinfect instruments in clinic.
Wang
Chuan
C
Shool of Public Health, West China Medical Center of Sichuan University, Chengdu 610041, China.
Xu
Xin
X
Yu
Qian
Q
Liu
Hengchuan
H
D001412:Bacillus subtilis / Q000187:drug effects; D004202:Disinfectants / Q000494:pharmacology; D004203:Disinfection / Q000379:methods*; D019723:Endoscopes; D004866:Equipment Contamination / Q000517:prevention & control*; D005976:Glutaral / Q000494:pharmacology*; D006801:Humans; D014465:Ultrasonics
D004202:Disinfectants; D005976:Glutaral