title: Clusters of regulatory signals for RNA polymerase II transcription associated with Alu family repeats and CpG islands in human promoters.
abstract: Primate genomes contain a very large number of short interspersed GC-rich repeats of the Alu family, which are abundant in introns and intergenic spacers but also present in 5' flanking regions of genes enriched in binding motifs (BMs) for transcription factors and frequently containing CpG islands. Here we studied whether CpG islands located in promoters of human genes overlap with Alu repeats and with clusters of BMs for the zinc-finger transcription factors Sp1, estrogen receptor alpha, and YY1. The presence of estrogen-response elements in Alu was shown earlier and here we confirm the presence in the consensus Alu sequence of the binding sites for Sp1 and YY1. Analyzing >5000 promoters from the two databases we found that Alu sequences are underrepresented in promoters compared to introns and that approximately 4% of CpG islands located within the -1000 to +200 segments of human promoters overlap with Alu repeats. Although this fraction was found to be lower for proximal segments of promoters (-500 to +100), our results indicate that a significant number (>1000) of all human genes may be controlled by Alu-associated CpG islands. Analysis of clustering of potential BMs for the indicated transcription factors within some promoters also suggests that the Alu family contributed to the evolution of transcription cis-regulatory modules in the human genome. It is important that among Alu sequences overlapping with CpG islands in promoters a large fraction of members of the old Alu subfamilies is found, suggesting extensive retroposon-assisted regulatory genome evolution during the divergence of the primates.
authors.affiliation: Institute of Biochemistry, Free University of Berlin, Thielallee 63, D-14195, Berlin-Dahlem, Germany.
authors.forename: Shiao-Li
authors.initials: SL
authors.lastname: Oei
authors.forename: Victor S
authors.initials: VS
authors.lastname: Babich
authors.forename: Vassily I
authors.initials: VI
authors.lastname: Kazakov
authors.forename: Nadezhda M
authors.initials: NM
authors.lastname: Usmanova
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Kropotov
authors.forename: Nikolai V
authors.initials: NV
authors.lastname: Tomilin
mesh_terms: D024506:5' Flanking Region / Q000235:genetics; D020087:Alu Elements / Q000235:genetics*; D001665:Binding Sites; D018899:CpG Islands / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D050981:Erythroid-Specific DNA-Binding Factors; D047628:Estrogen Receptor alpha; D006801:Humans; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012319:RNA Polymerase II / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D020218:Response Elements / Q000235:genetics; D016329:Sp1 Transcription Factor / Q000737:chemistry / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism; D024363:Transcription Initiation Site; D014158:Transcription, Genetic / Q000235:genetics*; D050986:YY1 Transcription Factor; D016335:Zinc Fingers
chemical_list: D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D047628:Estrogen Receptor alpha; D011960:Receptors, Estrogen; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; D050986:YY1 Transcription Factor; C494698:YY1 protein, human; D012319:RNA Polymerase II