title: Segregation of two glutaminase isoforms in islets of Langerhans.
abstract: Despite the importance of glutamatergic signalling in the co-ordination of hormone secretion, the identity of the enzyme for the production of glutamate in beta-cells is still unresolved. We have found that the endocrine pancreas co-expresses two isoforms of GA (glutaminase), denoted as kidney-type (KGA) and liver-type (LGA), with a complementary cellular pattern of expression. Whereas KGA was mainly present in alpha-cells, LGA was very abundant in beta-cells. This spatial segregation may have important functional implications, facilitating a differential regulation of glutamate production in insulin- and glucagon-secreting cells.
authors.affiliation: Departamento de Biología Celular, Genética y Fisiología, Facultad de Ciencias, Universidad de Málaga, 29071 Málaga, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Baglietto-Vargas
authors.forename: Juan F
authors.initials: JF
authors.lastname: López-Téllez
authors.forename: Inés
authors.initials: I
authors.lastname: Moreno-González
authors.forename: Antonia
authors.initials: A
authors.lastname: Gutiérrez
authors.forename: J Carlos
authors.initials: JC
authors.lastname: Aledo
mesh_terms: D000818:Animals; D005972:Glutaminase / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D007801:Langerhans Cells / Q000201:enzymology*; D008099:Liver / Q000201:enzymology; D008297:Male; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007527:Isoenzymes; D005972:Glutaminase