Different meningitis-causing bacteria induce distinct inflammatory responses on interaction with cells of the human meninges.
The interactions of bacterial pathogens with cells of the human leptomeninges are critical events in the progression of meningitis. An in vitro model based on the culture of human meningioma cells was used to investigate the interactions of the meningeal pathogens Escherichia coli K1, Haemophilus influenzae, Neisseria meningitidis and Streptococcus pneumoniae. A rank order of association with meningioma cells was observed, with N. meningitidis showing the highest levels of adherence, followed by E. coli, S. pneumoniae and H. influenzae. Neisseria meningitidis and H. influenzae did not invade meningioma cells or induce cell death, but induced a concentration-dependent secretion of inflammatory mediators. Neisseria meningitidis induced higher levels of IL-6, MCP-1, RANTES and GM-CSF than H. influenzae, but there was no significant difference in the levels of IL-8 induced by both pathogens. Streptococcus pneumoniae was also unable to invade meningioma cells, but low concentrations of bacteria failed to stimulate cytokine secretion. However, higher concentrations of pneumococci led to cell death. By contrast, only E. coli K1 invaded meningioma cells directly and induced rapid cell death before an inflammatory response could be induced. These data demonstrate that the interactions of different bacterial pathogens with human meningeal cells are distinct, and suggest that different intervention strategies may be needed in order to prevent the morbidity and mortality associated with bacterial meningitis.
Fowler
Mark I
MI
Molecular Microbiology and Infection, Division of Infection, Inflammation and Repair, University of Southampton Medical School, Southampton General Hospital, Southampton SO16 6YD, UK.
Weller
Roy O
RO
Heckels
John E
JE
Christodoulides
Myron
M
D001422:Bacterial Adhesion; D016923:Cell Death; D045744:Cell Line, Tumor; D018932:Chemokine CCL2 / Q000378:metabolism; D018946:Chemokine CCL5 / Q000378:metabolism; D015169:Colony Count, Microbial; D016207:Cytokines / Q000378:metabolism*; D003593:Cytoplasm / Q000382:microbiology; D004926:Escherichia coli / Q000472:pathogenicity*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006193:Haemophilus influenzae / Q000472:pathogenicity*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D008578:Meninges / Q000166:cytology / Q000276:immunology / Q000382:microbiology*; D008579:Meningioma; D016920:Meningitis, Bacterial / Q000276:immunology / Q000382:microbiology; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D009345:Neisseria meningitidis / Q000472:pathogenicity*; D013296:Streptococcus pneumoniae / Q000472:pathogenicity*
D018932:Chemokine CCL2; D018946:Chemokine CCL5; D016207:Cytokines; D015850:Interleukin-6; D016209:Interleukin-8; D016178:Granulocyte-Macrophage Colony-Stimulating Factor