title: High-energy collision-induced dissociation of oxosteroids derivatised to Girard hydrazones.
abstract: Neutral oxosteroids have been derivatised with Girard T and P hydrazine reagents to give the corresponding Girard hydrazone quaternary ammonium salts. Both Girard T (GT) and Girard P (GP) hydrazones of oxosteroids give very intense [M](+) ion signals in electrospray (ES) mass spectra and fragment within the ES interface and collision cell to give characteristic fragment ions. GT and GP derivatives give informative high-energy collision-induced dissociation spectra, from which the structure of the precursor oxosteroid can be determined. Both charge-remote and charge-mediated mechanisms are responsible for the formation of the fragment ions at high collision-energy.
authors.affiliation: Department of Pharmaceutical & Biological Chemistry, The School of Pharmacy, University of London, 29-39 Brunswick Square, London WC1N 1AX, UK.
authors.forename: William J
authors.initials: WJ
authors.lastname: Griffiths
authors.forename: Gunvor
authors.initials: G
authors.lastname: Alvelius
authors.forename: Suya
authors.initials: S
authors.lastname: Liu
authors.forename: Jan
authors.initials: J
authors.lastname: Sjövall
mesh_terms: D006835:Hydrazones / Q000032:analysis / Q000737:chemistry*; D007664:Ketosteroids / Q000737:chemistry*; D007700:Kinetics; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization; D013739:Testosterone / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D006835:Hydrazones; D007664:Ketosteroids; D013739:Testosterone