A high-throughput monoamine oxidase inhibition assay using liquid chromatography with tandem mass spectrometry.
A highly efficient method utilizing liquid chromatography with tandem mass spectrometry (LC/MS/MS) was developed and employed for high-throughput screening of compounds for monoamine oxidase (MAO) inhibition. The method used kynuramine as a common substrate for both MAO-A and MAO-B in incubations, and the 4-hydroxyquinoline (4-HQ) resulting from deamination of kynuramine followed by intramolecular condensation was analyzed using LC/MS/MS; formation of 4-HQ was used as the marker of MAO activity to evaluate the effects of test compounds. Isocratic liquid chromatography was applied to reduce the run time to 2 min. Because of the high specificity and sensitivity of detection of 4-HQ by LC/MS/MS, this method was able to use MAO enzymes at very low concentrations and to perform short incubations; as a result, consumable cost was minimized, and sample preparations were completely avoided. The inhibition data are highly reproducible, and the IC(50) values determined by the method are in good agreement with literature values. The results suggest that this method is very robust and can be used as a generic approach to screen for MAO inhibitors in drug discovery.
Yan
Zhengyin
Z
Division of Drug Discovery, Johnson & Johnson Pharmaceutical Research & Development, LLC, Spring House, PA 19477-0779, USA. zyan@prdus.jnj.com
Caldwell
Gary W
GW
Zhao
Boyu
B
Reitz
Allen B
AB
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D006912:Hydroxyquinolines / Q000032:analysis; D007735:Kynuramine / Q000032:analysis; D008995:Monoamine Oxidase / Q000737:chemistry / Q000378:metabolism; D008996:Monoamine Oxidase Inhibitors / Q000145:classification / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D006912:Hydroxyquinolines; D008996:Monoamine Oxidase Inhibitors; D007735:Kynuramine; D008995:Monoamine Oxidase; C034010:4-hydroxyquinoline