[Antigenic map of the cytochrome b5 cytosol domain].
Because of the high conservativity of cytochrome b5 cytosolic domain (t-cytochrome b5) among mammalian species the antibodies against bovine t-cytochrome b5 were successfully obtained only from evolutionary distant species (chicken). Antigenic mapping of t-cytochrome b5 by the peptide scanning method showed the presence of a large set of linear B-epitopes of this protein. This is well consistent with high content of water-accessible amino acid residues and polypeptide chain turns in its molecule. Linear B-epitopes of t-cytochrome b5 are presumably located in sites with species specificity of the amino acid sequence, despite of the high-degree surface exposure of the highly conservative site, which is probably responsible for the interaction with cytochrome P450. It can be explained by the absence of the effective T-helper support of the antibody response to amino acid sequence sites that are identical to own proteins of an organism. Nevertheless, antibodies against bovine t-cytochrome b5 possess high cross-reactivity with regard to rabbit full-sized cytochrome b5 because of the presence of one identical linear B-epitope and cross-interactions between other antigenic sites with minor structural differences.
Kolesanova
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EF
Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, 10 Pogodinskaya ul., Moscow, 119121 Russia. ekol@ibmh.msk.su
Moshkovskiĭ
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SA
Romashko
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EI
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D002417:Cattle; D002645:Chickens; D015786:Cytochromes b5 / Q000737:chemistry* / Q000276:immunology; D003600:Cytosol / Q000201:enzymology; D000939:Epitopes; D008297:Male; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D017434:Protein Structure, Tertiary
D000906:Antibodies; D000939:Epitopes; D015786:Cytochromes b5