title: [Application of dot-immunogold filtration assay in detection of IgG in sera of trichinellosis patients].
abstract: OBJECTIVE
  To study a new quick method for detecting serum IgG against Trichinella spiralis (T.s).
  
  
  METHODS
  Membrane antigen of muscle larva of T.s was isolated and combined with the nitrocellulose membrane. Goat anti-human IgG was conjugated with the golden pellets of colloidal state which was used as marked antibody for the experiment of dot-immunogold filtration assay to examine specific IgG.
  
  
  RESULTS
  T.s IgG and its corresponding antigen reacted on the membrane by filtration, and the result could be observed with naked eyes within 10 min. In the examination of 76 clinical serum samples, the positive rate was 94.7%. It was not significantly higher than that of ELISA (chi 2 = 2.83, P > 0.05).
  
  
  CONCLUSIONS
  The dot-immunogold filtration assay is rapid and simple for performing and reading in the detection of IgG against T.s with high sensitivity and specificity.
authors.affiliation: Cellular and Molecular Immunology, Laboratory of Henan University, Kaifeng 475001.
authors.forename: Ying-jie
authors.initials: YJ
authors.lastname: Liu
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.forename: Yao-hua
authors.initials: YH
authors.lastname: Qu
authors.forename: Yong
authors.initials: Y
authors.lastname: Zheng
authors.forename: Yan-hong
authors.initials: YH
authors.lastname: Liu
authors.forename: Yong-li
authors.initials: YL
authors.lastname: Zhang
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood; D012680:Sensitivity and Specificity; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D007074:Immunoglobulin G