title: Unfolding intermediate in the peroxisomal flavoprotein D-amino acid oxidase.
abstract: The flavoenzyme d-amino acid oxidase (DAAO) from Rhodotorula gracilis is a peroxisomal enzyme and a prototypical member of the glutathione reductase family of flavoproteins. DAAO is a stable homodimer with a FAD molecule tightly bound to each 40-kDa subunit. In this work, the urea-induced unfolding of dimeric DAAO was compared with that of a monomeric form of the same protein, a deleted dimerization loop mutant. By using circular dichroism spectroscopy, protein and flavin fluorescence, 1,8-anilinonaphtalene sulfonic acid binding and activity assays, we demonstrated that the urea-induced unfolding of DAAO is a three-state process, yielding an intermediate, and that this process is reversible. The intermediate species lacks the catalytic activity and the characteristic tertiary structure of native DAAO but has significant secondary structure and retains flavin binding. Unfolding of DAAO proceeds through formation of an expanded, partially unfolded inactive intermediate, characterized by low solubility, by increased exposure of hydrophobic surfaces, and by increased sensitivity to trypsin of the beta-strand F5 belonging to the FAD binding domain. The oligomeric state does not modify the inferred folding process. The strand F5 is in contact with the C-terminal alpha-helix containing the Ser-Lys-Leu sequence corresponding to the type 1 peroxisomal targeting signal, and this structural element interacts with the N-terminal betaalphabeta flavin binding motif (Rossmann fold). The expanded conformation of the folding intermediate (and in particular the higher disorder of the mentioned secondary structure elements) could match the structure of the inactive holoenzyme required for in vivo trafficking of DAAO through the peroxisomal membrane.
authors.affiliation: Department of Structural and Functional Biology, University of Insubria, via J. H. Dunant 3, Varese 21100, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Caldinelli
authors.forename: Stefania
authors.initials: S
authors.lastname: Iametti
authors.forename: Alberto
authors.initials: A
authors.lastname: Barbiroli
authors.forename: Francesco
authors.initials: F
authors.lastname: Bonomi
authors.forename: Luciano
authors.initials: L
authors.lastname: Piubelli
authors.forename: Pasquale
authors.initials: P
authors.lastname: Ferranti
authors.forename: Gianluca
authors.initials: G
authors.lastname: Picariello
authors.forename: Mirella S
authors.initials: MS
authors.lastname: Pilone
authors.forename: Loredano
authors.initials: L
authors.lastname: Pollegioni
mesh_terms: D020816:Amino Acid Motifs; D002384:Catalysis; D002853:Chromatography, Liquid; D002942:Circular Dichroism; D003605:D-Amino-Acid Oxidase / Q000737:chemistry*; D019281:Dimerization; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000378:metabolism; D005420:Flavoproteins / Q000737:chemistry*; D007930:Leucine / Q000737:chemistry; D008239:Lysine / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008856:Microscopy, Fluorescence; D008956:Models, Chemical; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D020675:Peroxisomes / Q000378:metabolism*; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012248:Rhodotorula / Q000201:enzymology; D013053:Spectrophotometry; D013696:Temperature; D013816:Thermodynamics; D014357:Trypsin / Q000494:pharmacology; D014508:Urea / Q000737:chemistry / Q000494:pharmacology
chemical_list: D005420:Flavoproteins; D014508:Urea; D003605:D-Amino-Acid Oxidase; D014357:Trypsin; D007930:Leucine; D008239:Lysine