Involvement of penaeidins in defense reactions of the shrimp Litopenaeus stylirostris to a pathogenic vibrio.
The present study reports for the first time the involvement of an antimicrobial peptide in the defense reactions of a shrimp infected by a pathogenic Vibrio, Vibrio penaeicida. New members of the penaeidin family were characterized in the shrimp Litopenaeus stylirostris by RT-PCR and RACE-PCR from hemocyte total RNAs, and by mass spectrometry detection and immunolocalization of mature peptides in shrimp hemocytes. In infected shrimps, bacteria and penaeidin distribution colocalized in the gills and the lymphoid organ that represented the main infected sites. Moreover, the shrimp immune response to infection involved massive hemocyte recruitment to infection sites where released penaeidin may participate in the isolation and elimination of the bacteria, We show that the ability of the shrimps to circumvent shrimp infections is closely related to a recovery phase based on the hematopoietic process.
Munoz
M
M
'Défense et Résistance chez les Invertébrés Marins', CC 80, Ifremer/CNRS/Université de Montpellier 2, 2 Place Eugène Bataillon, 34095 Montpellier, France.
Vandenbulcke
F
F
Garnier
J
J
Gueguen
Y
Y
Bulet
P
P
Saulnier
D
D
Bachère
E
E
D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000378:metabolism; D001483:Base Sequence; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000033:anatomy & histology / Q000378:metabolism / Q000382:microbiology*; D010455:Peptides; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014733:Vibrio / Q000378:metabolism*; D014735:Vibrio Infections
D000890:Anti-Infective Agents; D010455:Peptides; D020033:Protein Isoforms; D011506:Proteins; C109157:penaeidin 2; C109158:penaeidin 3