title: Porphyrin metabolism in lymphocytes of miners exposed to diesel exhaust at oil shale mine.
abstract: The present study was carried out on the evaluation and application of new biomarkers for populations exposed to occupational diesel exhaust at oil shale mines. Since not only genotoxic effects may play an important role in the generation of tumors, the level of porphyrin metabolism was proposed as a biomarker of diesel exhaust exposure effects. The data on determination of 5-aminolevulinic acid (ALA) synthesis and heme formation in lymphocytes from groups of 50 miners exposed to diesel exhaust and 50 unexposed surface workers of oil shale mine are presented. All workers were examined and interviewed using structured questionnaires. The levels of benzene, carbon monoxide and nitric oxides in air as well as concentrations of 1-nitropyrene and elemental carbon in particulate matter were used for evaluation of exposure to diesel exhaust in mine. The levels of ALA and protoporphyrin (PP), activities of ALA synthetase (ALA-S) and ferrochelatase (FC), as well as levels of PP associated with DNA (PP/DNA) were investigated in lymphocytes spectrophotometrically. Significant differences in activity of ALA synthesis and heme formation between exposed miners and surface workers were found (207+/-23 vs. 166+/-14 pmol/10(6) lymp./30' for ALA-S and 46.1+/-3.8 vs. 54.8+/-4.1 pmol/10(6) lymp./60' for FC activities, respectively, P<0.001). ALA-S activity was higher and ALA accumulated in lymphocytes of exposed miners. Inhibition of FC activity caused PP cellular accumulation and an increase in the PP/DNA level (P<0.05). Tobacco smoking led to the increase of ALA biosynthesis in lymphocytes of both surface and underground smokers. The comparison of data obtained for non-smokers and smokers of both groups of workers has shown a significant difference (P<0.05). The work duration of underground or surface workers did not significantly influence the investigated biochemical parameters. The determination of ALA synthesis in lymphocytes could be a useful biomonitoring index of organism sensitivity to underground working. The alterations of PP levels, FC activity and PP/DNA association in peripheral lymphocytes of miners illustrate the harmful effects of exposure to diesel exhaust.
authors.affiliation: Laboratory of Environmental Carcinogens, Institute of Experimental and Clinical Medicine, Hiiu 42, Tallinn 11619, Estonia. muzyka@ekmi.ee
authors.forename: V
authors.initials: V
authors.lastname: Muzyka
authors.forename: P T J
authors.initials: PT
authors.lastname: Scheepers
authors.forename: S
authors.initials: S
authors.lastname: Bogovski
authors.forename: I
authors.initials: I
authors.lastname: Lang
authors.forename: N
authors.initials: N
authors.lastname: Schmidt
authors.forename: V
authors.initials: V
authors.lastname: Ryazanov
authors.forename: T
authors.initials: T
authors.lastname: Veidebaum
mesh_terms: D000328:Adult; D000393:Air Pollutants / Q000009:adverse effects*; D000622:Aminolevulinic Acid / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D016022:Case-Control Studies; D005742:Gasoline / Q000009:adverse effects*; D006801:Humans; D008214:Lymphocytes / Q000502:physiology*; D008875:Middle Aged; D008906:Mining; D016273:Occupational Exposure; D010578:Petroleum; D017319:Photosensitizing Agents / Q000032:analysis; D011166:Porphyrins / Q000032:analysis / Q000378:metabolism*; D001335:Vehicle Emissions
chemical_list: D000393:Air Pollutants; D015415:Biomarkers; D005742:Gasoline; D010578:Petroleum; D017319:Photosensitizing Agents; D011166:Porphyrins; D001335:Vehicle Emissions; D000622:Aminolevulinic Acid