Influence of a 12-nt insertion present in the 3' untranslated region of classical swine fever virus HCLV strain genome on RNA synthesis.
The function of a 12-nt insertion 'CTTTTTTCTTTT' in the 3' untranslated region (3'UTR) of the HCLV strain, a vaccine strain derived from the Shimen strain of classical swine fever virus (CSFV), was examined in vitro. RNA synthesis increased when the 12-nt insertion was deleted from the 3'UTR of the HCLV strain. RNA synthesis also decreased when the 12-nt insertion was introduced into the 3'UTR of the CSFV Shimen, virulent strain. Therefore, the 12-nt insertion present in the 3'UTR of the HCLV strain may be a marker for avirulence. It was also found that the predicted secondary structure of the 3'UTR of the HCLV strain was more stable when the 12-nt insertion was deleted, and that the 3'UTR of Shimen strain was more unstable when the 12-nt insertion was introduced. Replacement of the 12-nt insertion 'CTTTTTTCTTTT' by another 12-nt fragment 'ATTATTATTTAT' in the 3'UTR of HCLV strain facilitated RNA synthesis and stabilized the predicted secondary structure, which was also observed in the 3'UTR of the Shimen strain.
Xiao
Ming
M
Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, The Institute of Biodiversity Science, Fudan University, Shanghai, PR China.
Gao
Jufang
J
Wang
Yujing
Y
Wang
Xiaohui
X
Lu
Wenwei
W
Zhen
Yamei
Y
Chen
Jiakuan
J
Li
Bo
B
D020413:3' Untranslated Regions; D001483:Base Sequence; D006692:Classical Swine Fever Virus / Q000235:genetics* / Q000254:growth & development*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000302:isolation & purification / Q000378:metabolism; D017384:Sequence Deletion; D014764:Viral Proteins / Q000302:isolation & purification / Q000378:metabolism; D014765:Viral Vaccines; D014779:Virus Replication
D020413:3' Untranslated Regions; D009843:Oligoribonucleotides; D012367:RNA, Viral; D014764:Viral Proteins; D014765:Viral Vaccines; D012324:RNA-Dependent RNA Polymerase