title: Dibenzocyclooctadiene lignans from Schisandra chinensis protect primary cultures of rat cortical cells from glutamate-induced toxicity.
abstract: A methanolic extract of dried Schisandra fruit (Schisandra chinensis Baill.; Schisandraceae) significantly attenuated the neurotoxicity induced by L-glutamate in primary cultures of rat cortical cells. Five dibenzocyclooctadiene lignans (deoxyschisandrin, gomisin N, gomisin A, schisandrin, and wuweizisu C) were isolated from the methanolic extract; their protective effects against glutamate-induced neurotoxicity were then evaluated. Among the five lignans, deoxyschisandrin, gomisin N, and wuweizisu C significantly attenuated glutamate-induced neurotoxicity as measured by 1). an inhibition in the increase of intracellular [Ca(2+)]; 2). an improvement in the glutathione defense system, the level of glutathione, and the activity of glutathione peroxidase; and 3). an inhibition in the formation of cellular peroxide. These results suggest that dibenzocyclooctadiene lignans from Schisandra chinensis may possess therapeutic potential against oxidative neuronal damage induced by excitotoxin.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul, Korea.
authors.forename: So Ra
authors.initials: SR
authors.lastname: Kim
authors.forename: Mi Kyeong
authors.initials: MK
authors.lastname: Lee
authors.forename: Kyung Ah
authors.initials: KA
authors.lastname: Koo
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Sung
authors.forename: Na Gyong
authors.initials: NG
authors.lastname: Lee
authors.forename: George J
authors.initials: GJ
authors.lastname: Markelonis
authors.forename: Tae H
authors.initials: TH
authors.lastname: Oh
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Yang
authors.forename: Young Choong
authors.initials: YC
authors.lastname: Kim
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D034242:Cyclooctanes / Q000494:pharmacology*; D004149:Dioxoles / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D018698:Glutamic Acid / Q000633:toxicity*; D007424:Intracellular Fluid / Q000378:metabolism; D017705:Lignans / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009498:Neurotoxins / Q000633:toxicity; D010545:Peroxides / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D011083:Polycyclic Compounds / Q000494:pharmacology; D051381:Rats; D032141:Schisandra / Q000737:chemistry*
chemical_list: D034242:Cyclooctanes; D004149:Dioxoles; D017705:Lignans; D018696:Neuroprotective Agents; D009498:Neurotoxins; D010545:Peroxides; D010936:Plant Extracts; D011083:Polycyclic Compounds; C015499:schizandrin B; D018698:Glutamic Acid; C033585:schizandrol B; C034734:schizandrin A; C031409:schizandrin C; C011105:schizandrin; D002118:Calcium