Detecting and quantifying apoptosis in tissue sections.
Various techniques exist for the identification of apoptosis in tissue sections or intact cells. The use of simple morphology, electron microscopy, DNA-end labeling techniques, and immunochemical methods are reviewed, with a particular emphasis on in situ end-labeling. The analysis of apoptotic cells and methods for their quantification are also discussed.
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Vicki
V
Department of Pathology, Addenbrookes Hospital, Cambridge University, UK.
Hall
Peter A
PA
Coates
Philip J
PJ
D017209:Apoptosis; D004247:DNA / Q000378:metabolism; D053938:DNA Fragmentation; D007150:Immunohistochemistry / Q000379:methods*; D020287:In Situ Nick-End Labeling / Q000379:methods
D004247:DNA