Proton pathways, ligand binding and dynamics of the catalytic site in haem-copper oxygen reductases: a comparison between the three families.
Haem-copper oxygen reductases are the widest spread enzymes involved in aerobic respiratory chains, in Eukarya, Bacteria and Archaea. However, both the catalytic mechanism for oxygen reduction and its coupling to proton translocation remain to be fully understood. In this article we analyse the experimental data gathered in recent years for haem-copper reductases presenting features distinct from the mitochondrial-like enzymes. These features further support the classification of several families of haem-copper oxygen reductases based on their proton pathways and previously proposed by us [Biochim. Biophys. Acta 1505 (2001) 185], and allow to identify the minimal essential elements for these enzymes.
Pereira
Manuela M
MM
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Rua da Quinta Grande 6, Apt 127, 2780-156 Oeiras, Portugal.
Teixeira
Miguel
M
D020134:Catalytic Domain; D003576:Electron Transport Complex IV / Q000737:chemistry* / Q000145:classification / Q000378:metabolism*; D007700:Kinetics; D008024:Ligands; D009569:Nitric Oxide / Q000378:metabolism; D018892:Proton-Motive Force; D013816:Thermodynamics
D008024:Ligands; D009569:Nitric Oxide; D003576:Electron Transport Complex IV