title: Legionella pneumophila replication vacuole formation involves rapid recruitment of proteins of the early secretory system.
abstract: Legionella pneumophila vacuole biogenesis was analyzed by using a cell-free system. We show that calnexin, Sec22b, and Rab1 are recruited to the vacuole very shortly after bacterial uptake, and we have identified Rab1 as a potential host factor involved in the endoplasmic reticulum recruitment process.
authors.affiliation: Department of Molecular Biology and Microbiology, Howard Hughes Medical Institute, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Derré
authors.forename: Ralph R
authors.initials: RR
authors.lastname: Isberg
mesh_terms: D000818:Animals; D019556:COS Cells; D037281:Calnexin / Q000378:metabolism*; D002474:Cell-Free System; D004721:Endoplasmic Reticulum / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D066298:In Vitro Techniques; D016952:Legionella pneumophila / Q000254:growth & development* / Q000472:pathogenicity*; D008565:Membrane Proteins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020298:U937 Cells; D014617:Vacuoles / Q000378:metabolism* / Q000382:microbiology*; D020761:rab1 GTP-Binding Proteins / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D037281:Calnexin; D020761:rab1 GTP-Binding Proteins