Aberrant expression of laminin gamma 2 chain and its prognostic significance in intrahepatic cholangiocarcinoma according to growth morphology.
Laminin gamma 2 chain is an extracellular matrix protein that plays an important role in cell migration and tumor invasion. We report altered expression and characteristic localization of this chain in a series of 105 cases of intrahepatic cholangiocarcinomas examined immunohistochemically. All tumors were grossly classified into the following three types: intraductal growth type (n=9), periductal infiltrating type (n=8) and mass-forming type (n=88). The tumors exhibited three distinct staining types: basement membrane staining, cytoplasmic staining and stromal staining. The basement membranous staining of laminin gamma 2 chain was more frequent in biliary dysplasia, intraductal growth and periductal infiltrating type than in mass-forming type. The cytoplasmic staining of carcinoma cells was observed especially at the cancer-stromal interface or at the invasive front of tumors. Stromal staining of laminin gamma 2 chain was essentially localized in the stroma around cancer cells at the invasive area, and the expression was significantly correlated with tumor aggressive factors and a poor prognosis in patients with intrahepatic cholangiocarcinoma. We conclude that laminin gamma 2 chain exhibits aberrant expression in a stepwise manner through different aggressive stages of tumor progression.
Aishima
Shinichi
S
Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka Japan. saishima@surgpath.med.kyushu-u.ac.jp
Matsuura
Shuji
S
Terashi
Takahiro
T
Taguchi
Kenichi
K
Shimada
Mitsuo
M
Maehara
Yoshihiko
Y
Tsuneyoshi
Masazumi
M
D000368:Aged; D001650:Bile Duct Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D001653:Bile Ducts, Intrahepatic / Q000737:chemistry / Q000473:pathology*; D018281:Cholangiocarcinoma / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D005260:Female; D006801:Humans; D007797:Laminin / Q000096:biosynthesis*; D008297:Male; D015999:Multivariate Analysis; D011379:Prognosis; D016019:Survival Analysis; D015996:Survival Rate; D013997:Time Factors
C400567:LAMC2 protein, human; D007797:Laminin