title: Human E2F6 is alternatively spliced to generate multiple protein isoforms.
abstract: E2F6 protein belongs to the family of the E2F transcription factors. Here, we showed that the human E2F6 gene contains nine exons distributed along 20.4kbp of genomic DNA on chromosome 2 leading to the transcription of six alternatively spliced E2F6 mRNAs that encode four different E2F6 proteins. Moreover, we identified an E2F6 pseudogene localized on chromosome 22 completely spliced and devoid of exons 2, 3, and 4, and part of exons 1 and 5. Definition of the transcriptional initiation site and sequence analysis show that the gene contains a TATA less, CAAT less, GC-rich promoter with multiple transcription start sites. Regulatory elements necessary for basal transcription reside within a 134bp fragment as determined by transient transfection experiments.
authors.affiliation: CNRS UMR 8117, Institut de Biologie de Lille, 1 rue Calmette, BP 447, 59021 Lille, France.
authors.forename: Zoulika
authors.initials: Z
authors.lastname: Kherrouche
authors.forename: Yvan
authors.initials: Y
authors.lastname: De Launoit
authors.forename: Didier
authors.initials: D
authors.lastname: Monté
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D004247:DNA; D050701:E2F6 Transcription Factor; D005091:Exons; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011487:Protein Conformation; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics*; D011544:Pseudogenes; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*
chemical_list: D050701:E2F6 Transcription Factor; C491744:E2F6 protein, human; C111236:E2f6 protein, mouse; D020033:Protein Isoforms; D012333:RNA, Messenger; D014157:Transcription Factors; D004247:DNA