A theoretical study on Cu(II) binding modes and antioxidant activity of mammalian normal prion protein.
In this paper, the density functional theory (DFT) method B3LYP/LANL2DZ was used to calculate binding energies and electron affinities for various Cu(II) binding modes of mammalian normal prion protein (PrP(c)). The calculation results not only provide solid evidence to support one of the experimentally determined Cu(II) binding modes of PrP(c) but also shed new light on the normal function of the elusive protein; that is, PrP(c) is rather a Cu(II) transporter than an antioxidant. In addition, the employed theoretical methodology is also useful to investigate the metal chelating properties of other proteins and to rationally design Cu,Zn-superoxide dismutase mimics.
Ji
Hong-Fang
HF
Laboratory for Computational Biology and Shandong Provincial Research Center for Bioinformatic Engineering and Technique, Shandong University of Technology, Zibo 255049, People's Republic of China.
Zhang
Hong-Yu
HY
D000975:Antioxidants / Q000494:pharmacology*; D002614:Chelating Agents / Q000494:pharmacology; D003300:Copper / Q000493:pharmacokinetics*; D004583:Electrons; D005544:Forecasting; D008962:Models, Theoretical; D011328:Prions / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000494:pharmacology
D000975:Antioxidants; D002614:Chelating Agents; D011328:Prions; D003300:Copper; D013482:Superoxide Dismutase