title: Identification of a MAGE-1 peptide recognized by cytolytic T lymphocytes on HLA-B*5701 tumors.
abstract: "Cancer germline" genes such as those of the MAGE family are expressed in many tumors and in male germline cells but are silent in normal tissues. They encode shared tumor-specific antigens that have been used in therapeutic vaccination trials of cancer patients. We report the identification of a new MAGE-1-encoded peptide that is recognized by a cytolytic T-lymphocyte (CTL) clone on human leukocyte antigen (HLA)-B*5701. The sequence of the peptide, corresponding to position 102-112 of the MAGE-1 protein sequence, is ITKKVADLVGF. When tumor cells expressing MAGE-1 were transfected with HLA-B*5701, they were lyzed by the CTL clone, indicating that the peptide is processed in tumor cells and can, therefore, be used as a target for anti-tumoral vaccination.
authors.affiliation: Institute of Cellular Pathology, Cellular Genetics Unit, Université de Louvain, B-1200 Brussels, Belgium.
authors.forename: V
authors.initials: V
authors.lastname: Corbière
authors.forename: H
authors.initials: H
authors.lastname: Nicolay
authors.forename: V
authors.initials: V
authors.lastname: Russo
authors.forename: V
authors.initials: V
authors.lastname: Stroobant
authors.forename: V
authors.initials: V
authors.lastname: Brichard
authors.forename: T
authors.initials: T
authors.lastname: Boon
authors.forename: P
authors.initials: P
authors.lastname: van der Bruggen
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D045744:Cell Line, Tumor; D002472:Cell Transformation, Viral; D015235:HLA-B Antigens / Q000276:immunology*; D004854:Herpesvirus 4, Human; D006801:Humans; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins / Q000235:genetics / Q000276:immunology*; D010455:Peptides / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000951:Antigens, Neoplasm; D015235:HLA-B Antigens; C072011:MAGEA1 protein, human; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins; D010455:Peptides