Pharmacology of skin aging. Stimulation of glycosaminoglycan biosynthesis by L-fucose and fucose-rich polysaccharides, effect of in vitro aging of fibroblasts.
The effect of L-fucose and fucose-rich polysaccharides (FROP-s [Biomed. Pharmacother., 2003; 57: 187-94]) was investigated, using human skin fibroblast cultures at several passages. The cells were incubated with radioactive glucosamine for 24 h, followed by the determination of individual glycosaminoglycans (GAG-s) by selective hydrolysis using specific enzymes. The effect of L-fucose and of FROP-3 [Biomed. Pharmacother., in 2003; 57: 187-94], both at 1 and 10 microg/ml, added to the culture medium, was investigated. L-Fucose stimulated the incorporation of the tracer in heparan sulfates by fibroblasts at the ninth passage by 20%. FROP-3 stimulated incorporation in keratan sulfates by 45% by fibroblasts of the fifth passage. This effect was identical at both concentrations tested. For fibroblasts at the ninth passage FROP-3 stimulated incorporation in dermatan sulfate. This effect was dose dependent of the order of +67% at 1 microg/ml and +128% at 10 microg/ml. Incorporation in hyaluronan was also stimulated by about +27%. These stimulations of GAG-biosynthesis might play a role in the increase of total skin thickness of hairless rats treated with L-fucose, as well as in several other favorable results recorded for FROP-3 such as the increased hydration (resistance to pressure) and elasticity of human skin (Robert C, Robert AM, Robert L).
Isnard
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Laboratoire Universitaire de Recherche en Ophtalmologie, Faculte de Medecine Broussais-Hôtel Dieu, Universite Pierre et Marie Curie (Paris 6), 1, place du Parvis-Notre-Dame, 75181 Paris 4, France.
Fodil-Bourahla
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I
Robert
A M
AM
Robert
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L
D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005643:Fucose / Q000031:analogs & derivatives / Q000494:pharmacology*; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006801:Humans; D011134:Polysaccharides / Q000494:pharmacology; D015595:Skin Aging / Q000187:drug effects*
D006025:Glycosaminoglycans; D011134:Polysaccharides; D005643:Fucose