Hydrogen/deuterium exchange mass spectrometry for investigating protein-ligand interactions.
Amide hydrogen/deuterium exchange mass spectrometry is rapidly becoming a powerful method for high-resolution analyses of protein dynamics, structure, and function. Hydrogen/deuterium exchange approaches can provide information that greatly augments and refines information derived from high-resolution structural studies, and can provide detailed information on native protein structure when structural information is unavailable. Application of this method for rapid analyses of protein-ligand complexes could prove useful for studies of important disease-related protein complexes. The following review covers fundamentals of hydrogen/deuterium exchange and its applications to the study of protein-ligand complexes. In addition, hydrogen/deuterium exchange mass spectrometry studies on a protein-inhibitor complex are presented.
Garcia
Ricardo A
RA
Department of Medicine, University of California, San Diego, CA 92093-0613, USA. r5garcia@ucsd.edu
Pantazatos
Dennis
D
Villarreal
Francisco J
FJ
D000577:Amides / Q000737:chemistry; D003903:Deuterium / Q000737:chemistry*; D006629:Hirudins / Q000031:analogs & derivatives / Q000737:chemistry; D006859:Hydrogen / Q000737:chemistry*; D007700:Kinetics; D008024:Ligands; D013058:Mass Spectrometry; D008958:Models, Molecular; D008968:Molecular Conformation; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000295:instrumentation; D011994:Recombinant Proteins / Q000737:chemistry; D013917:Thrombin / Q000737:chemistry
D000577:Amides; D006629:Hirudins; D008024:Ligands; D011506:Proteins; D011994:Recombinant Proteins; D006859:Hydrogen; D003903:Deuterium; D013917:Thrombin; C083544:lepirudin