Surface-mediated proton-transfer reactions in membrane-bound proteins.
As outlined by Peter Mitchell in the chemiosmotic theory, an intermediate in energy conversion in biological systems is a proton electrochemical potential difference ("proton gradient") across a membrane, generated by membrane-bound protein complexes. These protein complexes accommodate proton-transfer pathways through which protons are conducted. In this review, we focus specifically on the role of the protein-membrane surface and the surface-bulk water interface in the dynamics of proton delivery to these proton-transfer pathways. The general mechanisms are illustrated by experimental results from studies of bacterial photosynthetic reaction centres (RCs) and cytochrome c oxidase (CcO).
Adelroth
Pia
P
Department of Biochemistry and Biophysics, The Arrhenius Laboratories for Natural Sciences, Stockholm University, Svante Arrhenius väg 12, SE-106 91 Stockholm, Sweden. piaa@dbb.su.se
Brzezinski
Peter
P
D004579:Electron Transport; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018892:Proton-Motive Force; D012242:Rhodobacter sphaeroides / Q000201:enzymology / Q000235:genetics; D014867:Water / Q000378:metabolism
D008565:Membrane Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D014867:Water; D003576:Electron Transport Complex IV