[Studies on the antigens of invasive stages of Plasmodium yoelii and Plasmodium berghei].
OBJECTIVE
To detect the rhoptry and surface proteins of invasive stages of Plasmodium yoelii and P. berghei with monoclonal antibodies.


METHODS
Subcellular localization of antigens was detected by IFA. The antigens of different stages of the two species malaria parasites were analyzed by Western blotting.


RESULTS
The antigens of rhoptry are very complicated. There are similar epitopes of the rhoptry proteins detected between the two species of Plasmodium. The similar epitopes were also detected between ookinetes and merozoites of P. yoelii, and ookinete antigens between the two species. But there are different antigens detected between merozoites and ookinetes in P. yoelii. The sporozoite surface antigen of P. yoelii was not detected in the ookinetes and merozoites in the same species.


CONCLUSIONS
There are similar epitopes in the rhoptry and surface antigens of different stages and different species of rodent malaria parasites. There are also distinct antigens among them.
Jin
Li-qun
LQ
Department of Parasitology, Shantou University Medical College, Shantou 515031.
Luo
Jian-min
JM
Fu
Yu-cai
YC
Xu
Shi-e
SE
Guo
Yan
Y
Xie
Lin-chong
LC
Tsuboi
T
T
Torii
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000953:Antigens, Protozoan / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D015153:Blotting, Western; D000939:Epitopes / Q000276:immunology; D019084:Fluorescent Antibody Technique, Indirect; D051379:Mice; D010962:Plasmodium berghei / Q000276:immunology*; D010967:Plasmodium yoelii / Q000276:immunology*; D015800:Protozoan Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D000939:Epitopes; D015800:Protozoan Proteins; C065352:rhoptry associated protein, Plasmodium