Toxicological data and growth characteristics of single post-feeding larvae and puparia of Calliphora vicina (Diptera: Calliphoridae) obtained from a controlled nordiazepam study.
Larvae of the Calliphora vicina (Diptera: Calliphoridae) were reared on artificial food spiked with different concentrations of nordiazepam. The dynamics of the accumulation and conversion of nordiazepam to its metabolite oxazepam in post-feeding larvae and empty puparia were studied. Analysis was performed using a previously developed liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. This method enabled the detection and quantitation of nordiazepam and oxazepam in single larvae and puparia. Both drugs could be detected in post-feeding larvae and empty puparia. In addition, the influence of nordiazepam on the development and growth of post-feeding larvae was studied. However, no major differences were observed for these parameters between the larvae fed on food containing nordiazepam and the control group. To our knowledge, this is the first report describing the presence of nordiazepam and its metabolite, oxazepam, in single Calliphora vicina larvae and puparia.
Pien
Karen
K
Department of Pathology, Academic Hospital, Free University of Brussels, Brussels, Belgium. karen.pien@az.vub.ac.be
Laloup
Marleen
M
Pipeleers-Marichal
Miriam
M
Grootaert
Patrick
P
De Boeck
Gert
G
Samyn
Nele
N
Boonen
Tom
T
Vits
Kathy
K
Wood
Michelle
M
D000818:Animals; D014151:Anti-Anxiety Agents / Q000493:pharmacokinetics / Q000494:pharmacology*; D002853:Chromatography, Liquid; D004175:Diptera / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D005554:Forensic Medicine; D007814:Larva / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D013058:Mass Spectrometry; D003708:Nordazepam / Q000493:pharmacokinetics / Q000494:pharmacology*; D010076:Oxazepam / Q000493:pharmacokinetics
D014151:Anti-Anxiety Agents; D003708:Nordazepam; D010076:Oxazepam