Circulating auto-antibody against hepatoma-derived growth factor (HDGF) in patients with ulcerative colitis.
OBJECTIVE
Various circulating auto-antibodies have been reported in patients with ulcerative colitis. Hepatoma-derived growth factor (HDGF) is a mitogen, localized dominantly in the nucleus of proliferating cells. In this study, we demonstrated the circulating anti-HDGF auto-antibody and investigated its clinical roles in patients with ulcerative colitis.


METHODS
Anti-HDGF IgG antibodies were measured by the enzyme-linked immunosorbent assay with recombinant HDGF in 20 healthy volunteers and 40 patients with ulcerative colitis.


RESULTS
Circulating anti-HDGF antibody was detected in the serum of a patient with total colitis by Western blotting. Anti-HDGF auto-antibodies were detected at 65.6% in the serum of patients with total/left-sided colitis, compared with healthy subjects at 10%. During active stage, the circulating anti-HDGF auto-antibodies were detected at a higher frequency of 78.3% than those in remission stage at 37.5%. Furthermore, the titers during active colitis were higher than those during the remission stage. Anti-HDGF auto-antibodies were not detected in any patients with proctitis.


CONCLUSIONS
These findings suggest that anti-HDGF auto-antibodies in the serum of patients with ulcerative colitis would help to classify the total/left-sided colitis from proctitis, and the serial measurement of the titer would also be a good marker for the active colitis.
Nahamura
Hideji
H
Department of Molecular Medicine, Osaka University Graduate School of Medicine, Suita, Osaka, Japan. nakamura@hyo-med.ac.jp
Yoshida
Kenya
K
Kishima
Yoshihiko
Y
Enomoto
Hirayuki
H
Uyama
Hirokazu
H
Kuroda
Toshifumi
T
Okuda
Yorihide
Y
Hirotani
Tomonori
T
Ito
Hiroaki
H
Kawase
Ichiro
I
D001323:Autoantibodies / Q000097:blood*; D015153:Blotting, Western; D003093:Colitis, Ulcerative / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000276:immunology* / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D011349:Proctitis / Q000276:immunology
D001323:Autoantibodies; D036341:Intercellular Signaling Peptides and Proteins; C051610:hepatoma-derived growth factor