Isopanduratin A from Kaempferia pandurata as an active antibacterial agent against cariogenic Streptococcus mutans.
An antibacterial compound active against Streptococcus mutans was isolated from Kaempferia pandurata and identified as isopanduratin A using 1H NMR, 13C NMR and EI-MS. The minimum inhibitory concentration (MIC) of isopanduratin A was 4 mg/l which was much lower than that of some other natural anticariogenic agents such as sanguinarine (12 mg/l), green tea extract and carvacrol (125 mg/l), thymol (250 mg/l) and isoeugenol and eucalyptol (500 mg/l). The bactericidal test showed that isopanduratin A completely inactivated S. mutans at 20 mg/l in 1 min. Significant inhibitory activity of isopanduratin A was also observed against S. sobrinus, S. sanguinis and S. salivarius with an MIC of 4 mg/l. Damage to the cell membrane and cell wall of S. mutans by isopanduratin A was shown using transmission electron microscopy (TEM). These results suggest that isopanduratin A could be employed as a potential antibacterial agent for preventing dental caries.
Hwang
Jae-Kwan
JK
Department of Biotechnology & Bioproducts Research Center, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-749, South Korea. jkhwang@yonsei.ac.kr
Chung
Jae-Youn
JY
Baek
Nam-In
NI
Park
Jung-Hee
JH
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D002473:Cell Wall / Q000187:drug effects; D003731:Dental Caries / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D008854:Microscopy, Electron; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D013295:Streptococcus mutans / Q000187:drug effects* / Q000235:genetics / Q000648:ultrastructure; D030521:Zingiberaceae / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D010936:Plant Extracts; C487865:isopanduratin A