title: Serum-Free Cryopreservation of Five Mammalian Cell Lines in Either a Pelleted or Suspended State.
abstract: Herein we have explored two practical aspects of cryopreserving cultured mammalian cells during routine laboratory maintenance. First, we have examined the possibility of using a serum-free, hence more affordable, cryopreservative. Using five mammalian lines (Crandell Feline Kidney, MCF7, A72, WI 38 and NB324K), we found that the serum-free alternative preserves nearly as efficiently as the serum-containing preservatives. Second, we compared cryostorage of those cells in suspended versus a pellet form using both aforementioned cryopreservatives. Under our conditions, cells were in general recovered equally well in a suspended versus a pellet form.
authors.affiliation: Biology Department, Chadron State College. Chadron, NE 69337. USA.
authors.forename: Joe
authors.initials: J
authors.lastname: Corsini
authors.forename: Christy
authors.initials: C
authors.lastname: Hacker
authors.forename: Charles
authors.initials: C
authors.lastname: Bare