Bioregulator Vilon-induced reactivation of chromatin in cultured lymphocytes from old people.
The effect of the synthetic peptide bioregulator Vilon on structural and facultative heterochromatin of cultured lymphocytes from old people has been studied. The data obtained indicate that Vilon (a) induces unrolling (deheterochromatinization) of total heterochromatin; (b) activates synthetic processes caused by the reactivation of ribosomal genes as a result of deheterochromatinization of nucleolus organizer regions; (c) releases the genes repressed due to the condensation of euchromatic regions forming facultative heterochromatin; (d) does not induce decondensation of pericentromeric structural heterochromatin. Our results indicate that Vilon causes progressive activation (deheterochromatinization) of the facultative heterochromatin with increased aging.
Lezhava
Teimuraz
T
Department of Genetics, Tbilisi State University, Tbilisi, Georgia. lezh@kheta.ge
Khavison
Vladimir
V
Monaselidze
Jamlet
J
Jokhadze
Tinatin
T
Dvalishvili
Nana
N
Bablishvili
Nino
N
Barbakadze
Shota
S
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D002152:Calorimetry, Differential Scanning; D002478:Cells, Cultured; D004151:Dipeptides / Q000494:pharmacology*; D005786:Gene Expression Regulation; D006570:Heterochromatin / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D011110:Polymorphism, Genetic; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism
D004151:Dipeptides; D006570:Heterochromatin; C415909:lysylglutamic acid