The 70 kDa heat shock protein suppresses matrix metalloproteinases in astrocytes.
The 70 kDa heat shock protein (Hsp70) is synthesized in response to a variety of stresses, including ischemia, and is thought to act as a molecular chaperone to prevent protein denaturation and facilitate protein folding. Matrix metalloproteinases (MMPs), a family of serine proteases, are also upregulated by ischemia and are thought to promote cell death and tissue injury. We examined the influence of Hsp70 on expression and activity of MMPs. Astrocyte cultures were prepared from neonatal mice and transfected with retroviral vectors containing hsp70 or lacZ or mock infected, then exposed to oxygen-glucose deprivation followed by reperfusion. Zymograms and Western blots showed that Hsp70 over-expression suppressed MMP-2 and MMP-9. These findings suggest that Hsp70 may protect by regulating MMPs.
Lee
Jong Eun
JE
Department of Anatomy, College of Medicine, Room 136-A, Yonsei University, 134 Shinchon-Dong, Seodaemun-Ku, Seoul 120-752, Korea. jelee@yumc.yonsei.ac.kr
Kim
Yeun Jung
YJ
Kim
Jong Youl
JY
Lee
Won Taek
WT
Yenari
Midori A
MA
Giffard
Rona G
RG
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D015153:Blotting, Western; D002478:Cells, Cultured; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000494:pharmacology*; D007763:Lac Operon / Q000235:genetics; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000235:genetics; D061965:Matrix Metalloproteinase Inhibitors; D020782:Matrix Metalloproteinases / Q000096:biosynthesis*; D051379:Mice; D008813:Mice, Inbred ICR; D012313:RNA; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012190:Retroviridae / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D018840:HSP70 Heat-Shock Proteins; D061965:Matrix Metalloproteinase Inhibitors; C034477:RNA primers; D012333:RNA, Messenger; D012313:RNA; D020782:Matrix Metalloproteinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9