P66(ShcA) interacts with MAPKAP kinase 2 and regulates its activity.
Three mitogen activated protein kinase-activated protein kinase 2 (MAPKAP kinase 2, MK2) interacting proteins were identified using a yeast two-hybrid approach. ShcA, a signaling phospho-protein, human polyhomeotic 2 (HPH2), a transcriptional regulator, and highly similar to smoothelin (HSTS), which is related to the cytoskeletal associated protein smoothelin, interact specifically with MK2. The interaction of MK2 with the 66 kDa isoform of ShcA, p66(ShcA), and HPH2 was confirmed using co-immunoprecipitation. MK2 is activated with p66(ShcA) co-expression and p66(ShcA) is an in vitro substrate for MK2, further demonstrating their association and suggesting a biological role for p66(Shc) in MK2 activation.
Yannoni
Yvonne M
YM
Department of Inflammation, Wyeth Research, 200 Cambridge Park Drive, Cambridge, MA 02140-2311, USA. yvonne.yannoni@abbott.com
Gaestel
Matthias
M
Lin
Lih-Ling
LL
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D010750:Phosphoproteins / Q000378:metabolism* / Q000502:physiology; D010766:Phosphorylation; D063151:Polycomb Repressive Complex 2; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D016415:Sequence Alignment; D055765:Shc Signaling Adaptor Proteins; D000071425:Src Homology 2 Domain-Containing, Transforming Protein 1; D014157:Transcription Factors / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D000099190:MAP-Kinase-Activated Kinase 2
D048868:Adaptor Proteins, Signal Transducing; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D063151:Polycomb Repressive Complex 2; D017346:Protein Serine-Threonine Kinases; D055765:Shc Signaling Adaptor Proteins; D000071425:Src Homology 2 Domain-Containing, Transforming Protein 1; D014157:Transcription Factors; D000099190:MAP-Kinase-Activated Kinase 2; C485977:PHC2 protein, human; C528024:SHC1 protein, human