Effective magnetic labeling of transplanted cells with HVJ-E for magnetic resonance imaging.
Magnetic labeling of transplanted cells permits us to monitor their localization non-invasively using MRI. Since most transfection agents for magnetic labeling have the same cationic charge as Fe(3+), the efficiency may be reduced. The hemagglutinating virus-envelope has no charge and utilizes membrane fusion activity to deliver internalized materials. In this study, we investigated the feasibility of using the envelope to incorporate paramagnetic Fe(3+) particles into PC12 cells and astrocytes. The envelope effectively labeled both cells with Fe(3+), which showed significant decreases of signal intensity in T2-weighted MRI. Labeled cells transplanted into the rat striatum were clearly visualized by T2*-weighted MRI at a magnetic field of 2 T. The results indicate that the hemagglutinating virus-envelope is a powerful tool for magnetic labeling.
Toyoda
Ken-Ichiro
K
Molecular Neuroscience Research Center, Shiga University of Medical Science, Setatsukinowa-cho, Otsu 520-2192, Japan.
Tooyama
Ikuo
I
Kato
Masanari
M
Sato
Haruhisa
H
Morikawa
Shigehiro
S
Hisa
Yasuo
Y
Inubushi
Toshiro
T
D000818:Animals; D001253:Astrocytes / Q000637:transplantation / Q000821:virology; D016380:Brain Tissue Transplantation / Q000379:methods*; D003342:Corpus Striatum / Q000033:anatomy & histology / Q000601:surgery; D003911:Dextrans; D052203:Ferrosoferric Oxide; D005822:Genetic Vectors / Q000008:administration & dosage* / Q000032:analysis; D006085:Graft Survival / Q000502:physiology; D007501:Iron / Q000008:administration & dosage / Q000032:analysis; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles; D008297:Male; D008561:Membrane Fusion / Q000502:physiology; D051379:Mice; D010087:Oxides / Q000008:administration & dosage / Q000032:analysis; D016716:PC12 Cells / Q000637:transplantation / Q000821:virology; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013194:Staining and Labeling / Q000379:methods*; D014760:Viral Fusion Proteins / Q000008:administration & dosage* / Q000032:analysis
D003911:Dextrans; D058185:Magnetite Nanoparticles; D010087:Oxides; D014760:Viral Fusion Proteins; D007501:Iron; C065507:ferumoxides; D052203:Ferrosoferric Oxide