Neuronal nitric oxide synthase gene polymorphism and IgE-mediated allergy in the Central European population.
BACKGROUND
Several findings suggest that nitric oxide (NO) plays a significant role in the regulation of the Th1/Th2 balance and contributes to the development of allergic diseases. Our study investigates a possible association of C/T transition located 276-bp downstream from the translation termination site in exon 29 of the human nitric oxide synthase type 1 (NOS1) gene with immunoglobulin E (IgE)-mediated allergic diseases in the Czech population.


METHODS
The study included 688 subjects - 368 patients with clinically manifested allergic diseases and 320 unrelated controls with negative familial history of asthma/atopy. The NOS1 genotypes were determined by polymerase chain reaction (PCR) and restriction analysis by Eco72I.


RESULTS
No significant differences were found for allele or genotype frequencies of the 5266 C/T polymorphism in exon 29 of the NOS1 gene between IgE-mediated allergic diseases (or asthma alone) and healthy subjects. However, this common polymorphism showed a significant association with signs of atopy, especially with total serum IgE levels [log(e) IgE levels (mean +/- SD): CC genotype = 4.34 +/- 1.40; CT genotype = 4.58 +/- 1.53; TT genotype = 5.01 +/- 1.61; P < 0.05).


CONCLUSIONS
Our findings suggest that NOS1 gene may participate in the pathogenesis of high total serum IgE levels in allergic diseases in our population. These findings provide support for NOS1 as a candidate gene for IgE-mediated allergy.
Hollá
L I
LI
Department of Pathological Physiology, Masaryk University Brno, Brno, Czech Republic.
Schüller
M
M
Bucková
D
D
Vácha
J
J
D000293:Adolescent; D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D003596:Cytosine; D018153:Czech Republic; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D006967:Hypersensitivity / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D008297:Male; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052248:Nitric Oxide Synthase Type I; D011110:Polymorphism, Genetic; D013941:Thymine
D007073:Immunoglobulin E; D003596:Cytosine; C496320:NOS1 protein, human; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; D013941:Thymine