Identification and characterization of trypanosome RNA-editing complex components.
This chapter describes the methods used to purify the RNA-editing complex, to identify the proteins by mass spectrometry, and to demonstrate the functions for some of the proteins.
Stuart
Kenneth
K
Seattle Biomedical Research Institute, and Department of Pathobiology, University of Washington, Seattle, USA.
Panigrahi
Aswini K
AK
Schnaufer
Achim
A
D000818:Animals; D001483:Base Sequence; D018929:Cell Culture Techniques; D002458:Cell Fractionation / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007202:Indicators and Reagents; D008928:Mitochondria / Q000235:genetics / Q000648:ultrastructure; D015800:Protozoan Proteins / Q000235:genetics / Q000302:isolation & purification; D017393:RNA Editing / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D016053:RNA, Protozoan / Q000235:genetics* / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000302:isolation & purification; D015183:Restriction Mapping / Q000379:methods; D014345:Trypanosoma / Q000235:genetics*; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000254:growth & development
D017931:DNA Primers; D007202:Indicators and Reagents; D015800:Protozoan Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan; D011994:Recombinant Proteins