title: Antigenic differences between H5N1 human influenza viruses isolated in 1997 and 2003.
abstract: To assess whether the antigenic properties of H5 hemagglutinin (HA) change over time due to antigenic drift, we produced a panel of monoclonal antibodies (mAbs) against the HA of the index H5N1 human influenza A virus, A/Hong Kong/156/97. By immunizing mice with a plasmid expressing this HA and boosting the initial immunization with cell lysates transfected with the plasmid, a total of six hybridomas producing HA-specific mAbs were established: four to the HA1 subunit with hemadsorption-inhibiting activity and two to the HA2 subunit. None of the mAbs to HA1 could bind to the HA of a recent human isolate, A/Hong Kong/213/2003, indicating that there are substantial antigenic differences between the H5N1 human influenza virus isolated in 1997 and that isolated in 2003.
authors.affiliation: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, The University of Tokyo, Japan.
authors.forename: Taisuke
authors.initials: T
authors.lastname: Horimoto
authors.forename: Nao
authors.initials: N
authors.lastname: Fukuda
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Iwatsuki-Horimoto
authors.forename: Yi
authors.initials: Y
authors.lastname: Guan
authors.forename: Wilina
authors.initials: W
authors.lastname: Lim
authors.forename: Malik
authors.initials: M
authors.lastname: Peiris
authors.forename: Shunji
authors.initials: S
authors.lastname: Sugii
authors.forename: Takato
authors.initials: T
authors.lastname: Odagiri
authors.forename: Masato
authors.initials: M
authors.lastname: Tashiro
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kawaoka
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D000940:Antigenic Variation / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D053124:Influenza A Virus, H5N1 Subtype; D009980:Influenza A virus / Q000276:immunology*; D051379:Mice; D010957:Plasmids / Q000276:immunology; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus