Fibroblast growth factor 1 is produced prior to apolipoprotein E in the astrocytes after cryo-injury of mouse brain.
We recently reported that fibroblast growth factor 1 (FGF-1) upregulates apolipoprotein E (apoE) synthesis and its secretion as high density lipoprotein (HDL) in cultured astrocytes potentially by an autocrine or paracrine mechanism [Biochim. Biopys. Acta 1589 (2002) 261]. In order to examine pathophysiological relevance of this reaction, we studied association of the production of FGF-1 and apoE in the post-injury mouse brain. After the spot-injury of the brain by liquid nitrogen, the surface size of the wound shrunk more rapidly in the C57BL/6 wild-type mice than the apoE-knock out C57BL/6 mice. Immunohistochemical analysis of the lesions revealed that production of FGF-1 was identified in the reactive astrocytes by the day 2 after the injury in both types of mouse, prior to the production of apoE confirmed by the day 4 in the wild-type. These findings were consistent with our in-vitro observations and hypothesis that FGF-1 upregulates apoE synthesis and subsequently HDL production in the reactive astrocytes by an autocrine or paracrine manner. FGF-1 thus would exert its effect after the CNS damage through apoE secretion.
Tada
Toyohiro
T
Department of Pathology, Nagoya City University School of Nursing, Nagoya 467-8601, Japan.
Ito
Jin-ichi
J
Asai
Michiyo
M
Yokoyama
Shinji
S
D000818:Animals; D001057:Apolipoproteins E / Q000032:analysis / Q000096:biosynthesis* / Q000172:deficiency; D001253:Astrocytes / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D016220:Fibroblast Growth Factor 1 / Q000032:analysis / Q000096:biosynthesis*; D005615:Freezing; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009584:Nitrogen / Q000494:pharmacology; D014945:Wound Healing / Q000502:physiology
D001057:Apolipoproteins E; D016220:Fibroblast Growth Factor 1; D009584:Nitrogen