title: Growth inhibition and mutagenesis induced in Escherichia coli by dihydropyrazines with DNA strand-cleaving activity.
abstract: Dihydropyrazine (DHP) causes DNA strand breaks in vitro. We evaluated the cytotoxic and genotoxic potential of DHP in Escherichia coli. DHP exposure dose-dependently caused inhibition of cell growth in the wild-type strain, death in recA and uvrB, and an increase in mutation frequency in uvrB. These findings indicate that DHP causes DNA strand breaks in vivo.
authors.affiliation: Department of Biochemistry, Miyazaki Medical College, Kihara, Kiyotake, Miyazaki 889-1692, Japan. stakechi@post.med.miyazaki-u.ac.jp
authors.forename: Shinji
authors.initials: S
authors.lastname: Takechi
authors.forename: Tadatoshi
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Hajime
authors.initials: H
authors.lastname: Nomura
authors.forename: Toshio
authors.initials: T
authors.lastname: Minematsu
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Nakayama
mesh_terms: D004249:DNA Damage; D004269:DNA, Bacterial / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D016296:Mutagenesis; D011719:Pyrazines / Q000633:toxicity*
chemical_list: D004269:DNA, Bacterial; D011719:Pyrazines