Alternative reversed-phase high-performance liquid chromatography method to analyse organic acids in dairy products.
A RP-HPLC method for the analysis of oxalic, citric, formic, succinic, orotic, uric, pyruvic, acetic, propionic, lactic and butyric acids in dairy products with a simple treatment of the sample has been developed. A gradient programme pumping phosphate buffer at pH 2.20 and acetonitrile was used to separate the compounds on a C18 column. Various parameters affecting analysis have been optimised to take < 18 min with an excellent linearity (R > 0.999). The precision was good (R.S.D. < 5%) and the recovery found close to 100%. Its application to analyse the quality of some dairy products has been investigated.
Tormo
M
M
Instituto Lactológico de Lekunberri, Plazaola 23, Lekunberri, Navarra 31870, Spain.
Izco
J M
JM
D000143:Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003611:Dairy Products / Q000032:analysis*; D009930:Organic Chemicals / Q000032:analysis*
D000143:Acids; D009930:Organic Chemicals