title: [Detection of anti-Ehrlichia antibodies and direct demonstration of Ehrlichia nucleic acid using the polymerase chain reaction (PCR) in patients in the Czech Republic].
abstract: OBJECTIVE
  In patients presenting symptoms with a suspicion of ehrlichiosis we determined antiehrlichia antibodies and investigated the presence of Ehrlichia nucleic acid in the plasma.
  
  
  METHODS
  In our group were 46 patients with tick sucks in their case history, who presented symptoms compatible with ehrlichiosis. Anti-Ehrlichia antibodies were determined by an indirect immunofluorescent test with a commercial kit from MRL Diagnostics. Ehrlichia DNA was detected using a nested PCR - the target sequence was a part of the antigen Anaplasma phagocytophilum.
  
  
  RESULTS
  Antibodies against HGE agents were demonstrated in 28 % of the patients; 10.5 % of the patients had in their serum antibodies reacting to the Ehrlichia chaffeensis antigen. The nucleic acid of A. phagocytophilum was detected in 11 % of the patients.
  
  
  CONCLUSIONS
  The Czech population is relatively often exposed to Ehrlichia infections. Although most cases are asymptomatic, we should bear in mind this diagnosis, especially in immunodeficient patients, where early treatment may prevent a complicated course of the disease.
authors.affiliation: Department of Infectious Diseases, 2nd Medical Faculty, Charles University, Bulovka Hospital, 18081 Prague 8, Czech Republic. lasikovs@fnb.cz
authors.forename: Sárka
authors.initials: S
authors.lastname: Lásiková
authors.forename: Lenka
authors.initials: L
authors.lastname: Moravcová
authors.forename: Dusan
authors.initials: D
authors.lastname: Pícha
authors.forename: Daniela
authors.initials: D
authors.lastname: Holecková
authors.forename: Emanuel
authors.initials: E
authors.lastname: Zdárský
mesh_terms: D000328:Adult; D041081:Anaplasma phagocytophilum / Q000302:isolation & purification; D000907:Antibodies, Bacterial / Q000097:blood*; D004269:DNA, Bacterial / Q000097:blood*; D018469:Ehrlichia chaffeensis / Q000302:isolation & purification*; D016873:Ehrlichiosis / Q000175:diagnosis*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction
chemical_list: D000907:Antibodies, Bacterial; D004269:DNA, Bacterial