The effect of CO2 laser irradiation on PDL cell attachment to resected root surfaces.
OBJECTIVE
The effect of CO2 laser irradiation on PDL attachment to resected root ends was evaluated.


METHODS
Forty dentin wafers were divided into 4 equal groups. Groups I and II were not subjected to laser irradiation. Groups III and IV were subjected to irradiation with CO2 laser (wave length 10.6 mm). PDL cells attachment to the resected root surface (lased/non lased) and cell attachment to the root end filling material (gutta percha and MTA) were evaluated utilizing immunoflouresence microscopy and SEM.


RESULTS
PDL cell attachment was observed on dentin surfaces (control samples) and dentin away from the laser strikes (irradiated samples). PDL cell attachment was observed on MTA but was absent on gutta percha.


CONCLUSIONS
Absence of PDL attachment to laser irradiated dentin could be attributed to morphological surface changes in dentin produced by laser irradiation.
Fayad
Mohamed I
MI
Department of Endodontics, University of Illinois, Chicago 60612-7212, USA. m_fayad@hotmail.com
Hawkinson
Robert
R
Daniel
Jon
J
Hao
Jianjun
J
D017607:Aluminum Compounds / Q000737:chemistry; D017610:Calcium Compounds / Q000737:chemistry; D002245:Carbon Dioxide; D002448:Cell Adhesion / Q000528:radiation effects; D002478:Cells, Cultured; D003804:Dentin / Q000166:cytology / Q000528:radiation effects; D004338:Drug Combinations; D005455:Fluorescent Antibody Technique; D006171:Gutta-Percha / Q000737:chemistry; D006801:Humans; D007834:Lasers; D008855:Microscopy, Electron, Scanning; D010087:Oxides / Q000737:chemistry; D010513:Periodontal Ligament / Q000166:cytology / Q000528:radiation effects*; D012387:Root Canal Filling Materials / Q000737:chemistry; D017640:Silicates / Q000737:chemistry; D013499:Surface Properties; D014092:Tooth Root / Q000166:cytology / Q000528:radiation effects*
D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D002245:Carbon Dioxide; D006171:Gutta-Percha