Use of apple seed meal as a new source of beta-glucosidase for enzymatic glucosylation of 4-substituted benzyl alcohols and tyrosol in monophasic aqueous-dioxane medium.
A facile method for enzymatic glycosylation of 4-substituted benzyl alcohols and tyrosol with glucose in a monophasic aqueous-dioxane medium was reported, using a crude meal of apple seed as a new catalyst. The corresponding beta-d-glucosides were synthesized in moderate yields (13.1-23.1%), among which the salidroside was obtained in 15.8% yield.
Min Tong
Ai
A
Laboratory of Biocatalysis and Bioprocessing, East China University of Science and Technology, Shanghai 200237, China.
Ya Lu
Wen
W
He Xu
Jian
J
Qiang Lin
Guo
G
D019905:Benzyl Alcohol / Q000378:metabolism*; D004146:Dioxanes / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry* / Q000196:embryology; D006031:Glycosylation; D009682:Magnetic Resonance Spectroscopy; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000378:metabolism*; D012639:Seeds / Q000201:enzymology*; D021241:Spectrometry, Mass, Electrospray Ionization; D017550:Spectroscopy, Fourier Transform Infrared; D001617:beta-Glucosidase / Q000378:metabolism*
D004146:Dioxanes; C011867:4-hydroxyphenylethanol; D001617:beta-Glucosidase; C025223:1,4-dioxane; D019905:Benzyl Alcohol; D010626:Phenylethyl Alcohol