CLAG 9 is located in the rhoptries of Plasmodium falciparum.
Clag 9, a gene located on chromosome 9 of Plasmodium falciparum has previously been associated with the cytoadherence of parasitized erythrocytes to CD36. This gene is part of a multi-gene family found in all Plasmodium species studied to date. Using data from the Malaria Genome Sequencing Project, peptides specific for clag 9 were designed, synthesized and used to immunize mice. This antisera was used in Western blotting and immunofluorescence experiments to determine the cellular localization of CLAG 9 in the parasitized erythrocyte. Co-localization using immunofluorescence of wildtype and knockout parasites unequivocally shows that CLAG 9 is localized to the rhoptry organelles of P. falciparum.
Gardiner
Donald L
DL
Malaria Biology Laboratory, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research, 300 Herston Road, 4006 Herston, Queensland, Australia. donG@qimr.edu.au
Spielmann
Tobias
T
Dixon
Matthew W A
MW
Hawthorne
Paula L
PL
Ortega
Maria R
MR
Anderson
Karen L
KL
Skinner-Adams
Tina S
TS
Kemp
David J
DJ
Trenholme
Katharine R
KR
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D004912:Erythrocytes / Q000469:parasitology; D005455:Fluorescent Antibody Technique; D006801:Humans; D016778:Malaria, Falciparum / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D015388:Organelles / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D010963:Plasmodium falciparum / Q000235:genetics / Q000378:metabolism*; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
D000913:Antibodies, Protozoan; D015815:Cell Adhesion Molecules; D010455:Peptides; D015800:Protozoan Proteins; C407823:clag9 protein, Plasmodium falciparum