title: GP5 ectodomain epitope of porcine reproductive and respiratory syndrome virus, strain Lelystad virus.
abstract: Sera from pigs infected with the European porcine reproductive and respiratory syndrome virus (PRRSV), strain Lelystad virus (LV), were screened by indirect ELISA for antibodies that bound to a series of overlapping synthetic peptides covering amino acids (AA) 19-60 of the primary envelope glycoprotein (GP)5. Antibodies were detected that bound to an epitope(s) located in an ectodomain segment composed of AA 38-54. The antibodies strongly cross-reacted with peptides specific for the North American PRRSV VR2332. Antibodies with the same specificity were present in sera of pigs infected in the US with a European-like PRRSV. Competitive ELISA using overlapping 8-10-AA-long peptides confirmed that the epitope recognized by the antibodies from LV-infected pigs is located in the same segment as the neutralization epitopes previously identified for PRRSV VR2332 and the closely related arterivirus, lactate dehydrogenase-elevating virus (LDV). No antibodies were detected that bound to synthetic peptides representing further upstream GP5 segments that have been reported to carry neutralization or non-neutralization epitopes of some PRRSV isolates.
authors.affiliation: Department of Microbiology, University of Minnesota, MMC 196, Minneapolis, MN 55455, USA. plage001@umn.edu
authors.forename: Peter G W
authors.initials: PG
authors.lastname: Plagemann
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D003429:Cross Reactions; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology*; D009500:Neutralization Tests; D019151:Peptide Library; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D013552:Swine; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D019151:Peptide Library; D014759:Viral Envelope Proteins; C114789:glycoprotein 5, PRRSV