Comparative study of interaction of two type II ribosome-inactivating proteins and their A-chains with model membrane.
Both cinnamomin and ricin are type II ribosome-inactivating proteins. Cinnamomin is less cytotoxic compared with ricin. In order to clarify the mechanism of their different cytotoxicities, the interaction of cinnamomin and its A-chain with model membrane was investigated and compared with that of ricin and its A-chain. It was revealed that cinnamomin is less effective than ricin in interacting with model membrane. Cinnamomin A-chain interacts with model membrane much less violently than ricin A-chain. The differences in the interaction of cinnamomin, ricin or their A-chains with model membrane might at least in part indicate the different cytotoxicity between cinnamomin and ricin.
Xu
Hong
H
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, The Chinese Academy of Science. 320 Yue-yang Road, Shanghai 200031, China.
Wang
Bao-Zhong
BZ
Liu
Wang-Yi
WY
D020418:Algal Proteins; D000595:Amino Acid Sequence; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D054790:Ribosome Inactivating Proteins, Type 2; D012270:Ribosomes / Q000737:chemistry* / Q000187:drug effects; D012276:Ricin / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D016415:Sequence Alignment
D020418:Algal Proteins; D005656:Fungal Proteins; D008567:Membranes, Artificial; D010940:Plant Proteins; D011506:Proteins; D054790:Ribosome Inactivating Proteins, Type 2; C061930:cinnamomin, Phytophthora cinnamomi; D012276:Ricin