Complete mitochondrial DNA sequence of the Australian freshwater crayfish, Cherax destructor (Crustacea: Decapoda: Parastacidae): a novel gene order revealed.
The complete mitochondrial DNA sequence was determined for the Australian freshwater crayfish Cherax destructor (Crustacea: Decapoda: Parastacidae). The 15,895-bp genome is circular with the same gene composition as that found in other metazoans. However, we report a novel gene arrangement with respect to the putative arthropod ancestral gene order and all other arthropod mitochondrial genomes sequenced to date. It is apparent that 11 genes have been translocated (ND1, ND4, ND4L, Cyt b, srRNA, and tRNAs Ser(UGA), Leu(CUN), Ile, Cys, Pro, and Val), two of which have also undergone inversions (tRNAs Pro and Val). The 'duplication/random loss' mechanism is a plausible model for the observed translocations, while 'intramitochondrial recombination' may account for the gene inversions. In addition, the arrangement of rRNA genes is incompatible with current mitochondrial transcription models, and suggests that a different transcription mechanism may operate in C. destructor.
Miller
Adam D
AD
School of Ecology and Environment, Deakin University, P.O. Box 423, Warrnambool, Victoria 3280, Australia.
Nguyen
Thuy T T
TT
Burridge
Christopher P
CP
Austin
Christopher M
CM
D000818:Animals; D003400:Astacoidea / Q000235:genetics*; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D023061:Gene Order; D015321:Gene Rearrangement; D008969:Molecular Sequence Data; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA
D004272:DNA, Mitochondrial; D012335:RNA, Ribosomal; D012343:RNA, Transfer