title: The effect of cigarette smoke exposure on pulmonary metastatic disease in a murine model of metastatic breast cancer.
abstract: BACKGROUND
  Women who smoke have a higher rate of fatal breast cancer than nonsmoking women. An association between smoking and pulmonary metastases from breast cancer has been suggested by epidemiologic studies.
  
  
  OBJECTIVE
  To examine the relationship between exposure to cigarette smoke and pulmonary metastasis in a murine model of metastatic mammary cancer.
  
  
  METHODS
  Prospective, randomized study.
  
  
  METHODS
  Animal research laboratory.
  
  
  METHODS
  Female sexually mature BALB/cAnN mice.
  
  
  METHODS
  Mice were randomly divided into experimental and control groups. Experimental animals were exposed to cigarette smoke in specialized exposure chambers, at concentrations chosen to approximate active cigarette smoking. Control animals were exposed to filtered air. One week after the initiation of exposures, mouse mammary tumor cells (tumor cell line 4526) were injected into the tail veins of experimental animals at one of three concentrations (50,000, 100,000, or 150,000 cells per 100 micro L). Three weeks later, the mice were killed, and pulmonary metastases were counted and measured.
  
  
  RESULTS
  The mean metastatic burden in the lungs was consistently greater for smoke-exposed animals at each concentration of cells injected (at 50,000 cells per 100 micro L, 9.8 vs 4.8 micro m(3), respectively [p < 0.01]; at 100,000 cells per 100 micro L, 34.5 vs 17.4 micro m(3), respectively [p < 0.10]; and at 150,000 cells per 100 micro L, 54.0 vs 31.5 micro m(3), respectively [p < 0.05]). This was largely attributable to a significant increase in the number of metastatic nodules per animal (at 50,000 cells per 100 micro L, 8.7 vs 4.8, respectively [p < 0.001]; at 100,000 cells per 100 micro L, 24.3 vs 14.0, respectively [p > 0.10]; and at 150,000 cells per 100 micro L, 42.0 vs 20.1, respectively [p < 0.02]) rather than to a change in nodule size.
  
  
  CONCLUSIONS
  Cigarette smoke exposure is associated with an increase in the total pulmonary metastatic burden in this murine model of metastatic mammary cell cancer. This study provides experimental support for an adverse effect of smoking on the metastatic process and suggests a possible mechanism for smokers' increased breast cancer mortality.
authors.affiliation: Division of Pulmonary and Critical Care Medicine, University of California Davis School of Medicine, Davis, CA, USA. sxmurin@ucdavis.edu
authors.forename: Susan
authors.initials: S
authors.lastname: Murin
authors.forename: Kent E
authors.initials: KE
authors.lastname: Pinkerton
authors.forename: Neil E
authors.initials: NE
authors.lastname: Hubbard
authors.forename: Kent
authors.initials: K
authors.lastname: Erickson
mesh_terms: D000818:Animals; D005260:Female; D008175:Lung Neoplasms / Q000556:secondary*; D008325:Mammary Neoplasms, Experimental / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011446:Prospective Studies; D011897:Random Allocation; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
chemical_list: D014028:Tobacco Smoke Pollution