title: Purification of a fibrinolytic enzyme (myulchikinase) from pickled anchovy and its cytotoxicity to the tumor cell lines.
abstract: A fibrinolytic enzyme, myulchikinase, from a Korean seasoning ingredient, myul-chi-jeot-gal, has been purified to electrophoretic homogeneity. The molecular mass of the myulchikinase was estimated to about 28 kDa by SDS-PAGE and gel filtration. Amino acid sequence of the NH2-terminal of myulchikinase showed significant homology with other fibrinolytic enzymes including trypsin from starfish, katsuwokinase, and rat pancreatic elastase II. The purified myulchikinase hydrolyzed various synthetic substrates with different substrate specificity and cytotoxic to the tumor cell lines.
authors.affiliation: Department of Life Science and Biotechnology, Dong-Eu University, Busan 614-714, Korea. ykjeong@dongeui.ac.kr
authors.forename: Yong-Kee
authors.initials: YK
authors.lastname: Jeong
authors.forename: Woong Suk
authors.initials: WS
authors.lastname: Yang
authors.forename: Kwang Hyuk
authors.initials: KH
authors.lastname: Kim
authors.forename: Kyung Tae
authors.initials: KT
authors.lastname: Chung
authors.forename: Woo Hong
authors.initials: WH
authors.lastname: Joo
authors.forename: Jae Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Dong-Eun
authors.initials: DE
authors.lastname: Kim
authors.forename: Jeong Uck
authors.initials: JU
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001940:Breast / Q000187:drug effects / Q000473:pathology; D045744:Cell Line, Tumor / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D010450:Endopeptidases / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004789:Enzyme Activation; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005399:Fishes / Q000378:metabolism*; D005404:Fixatives / Q000737:chemistry; D006801:Humans; D020014:K562 Cells; D008223:Lymphoma / Q000473:pathology; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009369:Neoplasms / Q000473:pathology*; D017386:Sequence Homology, Amino Acid
chemical_list: D000970:Antineoplastic Agents; D005343:Fibrinolytic Agents; D005404:Fixatives; D010450:Endopeptidases; C492920:myulchikinase