[Determination of the functional activity, amount of C1 inhibitor, and autoantibodies as a tool for differential diagnosis of angioedema].
Aetiology of angioedema (and therefore the scheme of its treatment) can be different. Angioedema may be subdivided into four categories: hereditary and acquired angioedemas, allergies and vasculitis. To establish the reason of the hereditary and acquired form of angioedema analyses of functional activity of complement components, quantities and activity of C1 inhibitor, presence (or absence) autoantibodies to C1 inhibitor allow. Sorption of the puried enzymes of activated subcomponent C1s or plasmin on micropanels allows to connect specifically in cells of plate C1 inhibitor from serum and with the help conjugate of antibodies against C1 inhibitor with a horse-radish peroxidase to determine quantity of connected functionally active C1 inhibitor. Addition of this test-system ELISA system for determination of quantitative contents of C1 inhibitor in serum, and also systems for definition IgG, IgA and IgM autoantibodies against C1 inhibitor finishes creation of a necessary set of methods of differential diagnostics.
Andina
S S
SS
Gabrichevsky Moscow Scientific Research Institute of Epidemiology and Microbiology, Admirala Makarova, 10, Moscow, 125212 Russia.
Kozlov
L V
LV
D'iakov
V L
VL
D000799:Angioedema / Q000145:classification / Q000175:diagnosis* / Q000276:immunology; D001323:Autoantibodies / Q000097:blood*; D003174:Complement C1 Inactivator Proteins; D050718:Complement C1 Inhibitor Protein; D003937:Diagnosis, Differential; D006801:Humans; D007124:Immunoenzyme Techniques; D015843:Serpins / Q000097:blood* / Q000172:deficiency
D001323:Autoantibodies; D003174:Complement C1 Inactivator Proteins; D050718:Complement C1 Inhibitor Protein; C469952:SERPING1 protein, human; D015843:Serpins