In vitro RNA editing systems from higher plant chloroplasts.
RNA editing in higher plant chloroplasts involves C-to-U conversion at ~30 specific sites. In vitro systems supporting accurate editing have been developed from tobacco and pea chloroplasts. mRNA substrates labeled with 32P at C residues to be edited provide sensitive detection of editing activity in vitro. The present systems allow the rapid identification of cis-elements using mutated mRNA substrates and trans-acting factors by ultraviolet crosslinking.
Hirose
Tetsuro
T
School of Biomedical Science, Tokyo Medical and Dental University, Japan.
Miyamoto
Tetsuya
T
Obokata
Junichi
J
Sugiura
Masahiro
M
D001483:Base Sequence; D002458:Cell Fractionation / Q000379:methods; D002736:Chloroplasts / Q000235:genetics* / Q000648:ultrastructure; D003596:Cytosine; D018532:Pisum sativum / Q000235:genetics; D010944:Plants / Q000235:genetics*; D017393:RNA Editing / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics*; D012680:Sensitivity and Specificity; D014026:Nicotiana / Q000235:genetics; D014498:Uracil
D012333:RNA, Messenger; D018749:RNA, Plant; D014498:Uracil; D003596:Cytosine