Stimulation of reductive dechlorination of hexachlorobenzene in soil by inducing the native microbial activity.
The reductive dechlorination and behaviour of (14)C-hexachlorobenzene (HCB) was investigated in an arable soil. The activity of the native anaerobic microbial communities could be induced by saturating the soil with water. Under these conditions high rates of dechlorination were observed. After 20 weeks of incubation only 1% of the applied 14C-HCB could be detected in the fraction of extractable residues. Additional organic substances, like wheat straw and lucerne straw, however considerably delayed and reduced the dechlorination process in the soil. The decline of HCB was not only caused by dechlorination but also by the formation of non-extractable residues, whereby their amounts varied with time depending on the experimental conditions. Several dechlorination products were detected, indicating the following main HCB transformation pathway: HCB --> PCB --> 1,2,3,5-TeCB --> 1,3,5-TCB --> 1,3-DCB, with 1,3,5-TCB as main intermediate dechlorination product. The other TeCB-, TCB- and DCB-isomers were also detected in low amounts, showing the presence of more than one dechlorination pathway. Since the methane production rates were lowest when the dechlorination rates were highest, it can be assumed that methanogenic bacteria were not involved in the dechlorination process of HCB. The established 14C-mass balances show, that with increasing dechlorination and incubation times, the 14C-recoveries decreased.
Brahushi
Ferdi
F
GSF--National Research Center for Environment and Health, Institute of Soil Ecology, Neuherberg 85764, Germany.
Dörfler
Ulrike
U
Schroll
Reiner
R
Munch
Jean Charles
JC
D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002250:Carbon Radioisotopes; D002712:Chlorides; D002849:Chromatography, Gas; D006581:Hexachlorobenzene / Q000378:metabolism*; D007700:Kinetics; D008697:Methane; D012988:Soil Microbiology
D002250:Carbon Radioisotopes; D002712:Chlorides; D006581:Hexachlorobenzene; D008697:Methane