Induction of transcription factor c-myb expression in reactive astrocytes following intracerebroventricular kainic acid injection in mouse hippocampus.
The transcription factor c-myb is known to play an important role in the regulation of cellular proliferation and differentiation. Recently, the constitutive and aberrant expression of c-myb in the normal and Cu/Zn SOD mutant mouse brain was reported. However, the expression of c-myb in the process of reactive gliosis is not known yet. Here we report the delayed and protracted induction of c-myb in the brain of mice following kainic acid (KA) induced seizure. Our western blot analysis revealed that the amount of c-myb was dramatically increased in the brain 3 days after KA treatment. The induction of c-myb was sustained for more than 7 days after KA treatment. The c-myb immunoreactivity (IR) was restricted to neurons of the hippocampus in control mice. Three days after KA treatment, a strong c-myb IR was found in reactive astrocytes in the whole areas of the CA3 region. Thereafter, c-myb IR astrocytes were gradually concentrated around the CA3 region undergoing selective neuronal loss. A few c-myb IR astrocytes were continuously persisted in the CA3 region 14 days after KA treatment. These findings suggest a role of c-myb signal pathway in reactive gliosis in mice with KA induced seizure.
Jeon
Gye Sun
GS
Department of Anatomy, Seoul National University, College of Medicine, 28 Yongon-Dong, Chongno-Gu, Seoul 110-799, South Korea.
Shin
Dong Hoon
DH
Cho
Sa Sun
SS
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D020570:Genes, myb / Q000187:drug effects / Q000502:physiology*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D007276:Injections, Intraventricular; D007608:Kainic Acid / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
D014157:Transcription Factors; D007608:Kainic Acid