A multiresolution approach to orientation assignment in 3D electron microscopy of single particles.
Three-dimensional (3D) electron microscopy (3DEM) aims at the determination of the spatial distribution of the Coulomb potential of macromolecular complexes. The 3D reconstruction of a macromolecule using single-particle techniques involves thousands of 2D projections. One of the key parameters required to perform such a 3D reconstruction is the orientation of each projection image as well as its in-plane orientation. This information is unknown experimentally and must be determined using image-processing techniques. We propose the use of wavelets to match the experimental projections with those obtained from a reference 3D model. The wavelet decomposition of the projection images provides a framework for a multiscale matching algorithm in which speed and robustness to noise are gained. Furthermore, this multiresolution approach is combined with a novel orientation selection strategy. Results obtained from computer simulations as well as experimental data encourage the use of this approach.
Sorzano
C O S
CO
Escuela Politécnica Superior, Universidad San Pablo-CEU, Campus Urb., Madrid, Spain. coss@cnb.uam.es
Jonić
S
S
El-Bez
C
C
Carazo
J M
JM
De Carlo
S
S
Thévenaz
P
P
Unser
M
M
D000465:Algorithms; D001436:Bacteriorhodopsins / Q000737:chemistry; D018834:Chaperonin 60 / Q000737:chemistry; D003198:Computer Simulation; D021621:Imaging, Three-Dimensional / Q000379:methods*; D046911:Macromolecular Substances / Q000737:chemistry*; D008854:Microscopy, Electron / Q000379:methods*; D008958:Models, Molecular; D010316:Particle Size
D018834:Chaperonin 60; D046911:Macromolecular Substances; D001436:Bacteriorhodopsins