title: Identification of 36 kDa phosphoprotein in fibrous sheath of hamster spermatozoa.
abstract: In our previous studies (Fujinoki et al., 2001, 2003), we reported that two types of 36 kDa proteins, designated 36K-A protein and 36K-B protein, obtained from hamster sperm flagella, are associated with motility activation and phosphorylated in a cAMP-dependent manner at serine residues. In the present experiments, we focused on the hamster (Mesocricetus auratus) 36K-A protein, which was analyzed by peptide mass finger printing and amino acid sequencing. The results suggest that 36K-A protein is a pyruvate dehydrogenase E1 component beta subunit lacking the N-terminal 30 amino acids. Moreover, our results suggest that 36 K-A protein is localized in the fibrous sheath of the principal piece of hamster spermatazoa.
authors.affiliation: Department of Physiology, Dokkyo University School of Medicine, 880 kitakobayasi, Mibu, Tochigi 321-0293, Japan. fujinoki@dokkyomed.ac.jp
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Fujinoki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kawamura
authors.forename: Toshifusa
authors.initials: T
authors.lastname: Toda
authors.forename: Hideki
authors.initials: H
authors.lastname: Ohtake
authors.forename: Tadashi
authors.initials: T
authors.lastname: Ishimoda-Takagi
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Shimizu
authors.forename: Sadao
authors.initials: S
authors.lastname: Yamaoka
authors.forename: Makoto
authors.initials: M
authors.lastname: Okuno
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D006224:Cricetinae; D000242:Cyclic AMP / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis / Q000737:chemistry; D010750:Phosphoproteins / Q000737:chemistry / Q000302:isolation & purification*; D010766:Phosphorylation; D021122:Protein Subunits / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D030481:Pyruvate Dehydrogenase (Lipoamide) / Q000378:metabolism; D012694:Serine / Q000737:chemistry; D013082:Sperm Tail / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism; D013094:Spermatozoa / Q000737:chemistry*
chemical_list: D010455:Peptides; D010750:Phosphoproteins; D021122:Protein Subunits; D012694:Serine; D000242:Cyclic AMP; D030481:Pyruvate Dehydrogenase (Lipoamide); C111305:pyruvate dehydrogenase E1 beta subunit