title: Context-dependent effects of CB1 cannabinoid gene disruption on anxiety-like and social behaviour in mice.
abstract: Contrasting data were reported regarding the effects of cannabinoids on anxiety and social behaviour in both animals and humans. The cognitive effects of cannabinoids and their interactions with the HPA-axis raise the possibility that cannabinoid effects are context but not behaviour specific. To assess this hypothesis, we submitted CB1 receptor knock-out (CB1-KO) and wild-type (WT) mice to tests, which involved similar behaviours, but the behavioural context was different. The elevated plus-maze test was performed under less and more anxiogenic conditions, i.e. under low and high light, respectively. We also compared the social behaviour of the two genotypes in the resident/intruder and social interaction tests. Both tests represent a social challenge and induce similar behaviours, but involve different contexts. The behaviour of CB1-KO and WT mice was similar under low light, but CB1 gene disruption increased anxiety-like behaviour under the high light condition. CB1 gene disruption promoted aggressive behaviour in the home-cage, whereas it inhibited social behaviour in the unfamiliar cage. Thus, the anxiogenic-like effect was restricted to the more stressful unfamiliar environment. These data suggest that the effects of CB1 gene disruption were context and not behaviour specific. Novelty stress resulted in higher ACTH levels in CB1-KOs than in WTs, which suggests that context dependency occurred in conjunction with an altered HPA axis function. The present data at least partly explain contrasting effects of cannabinoids in different contexts as well as in different species and strains that show differential stress responses and coping strategies.
authors.affiliation: Institute of Experimental Medicine, 1083 Budapest, P.O. Box 67, Hungary. haller@koki.hu
authors.forename: J
authors.initials: J
authors.lastname: Haller
authors.forename: B
authors.initials: B
authors.lastname: Varga
authors.forename: C
authors.initials: C
authors.lastname: Ledent
authors.forename: I
authors.initials: I
authors.lastname: Barna
authors.forename: T F
authors.initials: TF
authors.lastname: Freund
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D001007:Anxiety / Q000235:genetics / Q000503:physiopathology*; D001522:Behavior, Animal; D005106:Exploratory Behavior / Q000502:physiology; D007398:Interpersonal Relations; D008027:Light; D008124:Locomotion / Q000235:genetics; D018782:Maze Learning / Q000187:drug effects / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D043884:Receptor, Cannabinoid, CB1 / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D012919:Social Behavior; D013997:Time Factors
chemical_list: D043884:Receptor, Cannabinoid, CB1; D000324:Adrenocorticotropic Hormone