title: Crystal structure of the YffB protein from Pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function.
abstract: BACKGROUND
  The yffB (PA3664) gene of Pseudomonas aeruginosa encodes an uncharacterized protein of 13 kDa molecular weight with a marginal sequence similarity to arsenate reductase from Escherichia coli. The crystal structure determination of YffB was undertaken as part of a structural genomics effort in order to assist with the functional assignment of the protein.
  
  
  RESULTS
  The structure was determined at 1.0 A resolution by single-wavelength anomalous diffraction. The fold is very similar to that of arsenate reductase, which is an extension of the thioredoxin fold.
  
  
  CONCLUSIONS
  Given the conservation of the functionally important residues and the ability to bind glutathione, YffB is likely to function as a GSH-dependent thiol reductase.
authors.affiliation: Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, MD 20850, USA. teplyako@umbi.umd.edu
authors.forename: Alexey
authors.initials: A
authors.lastname: Teplyakov
authors.forename: Sadhana
authors.initials: S
authors.lastname: Pullalarevu
authors.forename: Galina
authors.initials: G
authors.lastname: Obmolova
authors.forename: Victoria
authors.initials: V
authors.lastname: Doseeva
authors.forename: Andrey
authors.initials: A
authors.lastname: Galkin
authors.forename: Osnat
authors.initials: O
authors.lastname: Herzberg
authors.forename: Miroslawa
authors.initials: M
authors.lastname: Dauter
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Dauter
authors.forename: Gary L
authors.initials: GL
authors.lastname: Gilliland
mesh_terms: D053501:Arsenite Transporting ATPases; D001426:Bacterial Proteins / Q000737:chemistry*; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D016623:Ion Pumps / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000737:chemistry; D011490:Protein Disulfide Reductase (Glutathione) / Q000737:chemistry*; D017510:Protein Folding; D017434:Protein Structure, Tertiary / Q000502:physiology; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship
chemical_list: D001426:Bacterial Proteins; D016623:Ion Pumps; D009097:Multienzyme Complexes; D011490:Protein Disulfide Reductase (Glutathione); D053501:Arsenite Transporting ATPases