Evaluation of spectral imaging for plant cell analysis.
Fluorescence imaging at high spectral resolution is now a practical reality and has great promise in plant cell biology. Emission spectral curve data can be used computationally to distinguish spectrally similar fluorophores, or to remove autofluorescence, and to spectrally analyse autofluorescent molecules, which are especially abundant in plant tissues. Examples of these applications in plant cells are given, and a comparison is made between the current offerings in spectral imaging laser scanning confocal microscopes.
Berg
R H
RH
Integrated Microscopy Facility, Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, USA. rhberg@danforthcenter.org
D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D059828:Plant Cells; D010944:Plants / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods*
D005456:Fluorescent Dyes