title: Is histoaspartic protease a serine protease with a pepsin-like fold?
abstract: The primary structure of the so-called histoaspartic protease from Plasmodium falciparum has a very high percentage of identity and homology with the pepsin-like enzyme plasmepsin II. A homology modeling approach was used to calculate the three-dimensional structure of the enzyme. Molecular dynamics (MD) simulations were applied to find those structural properties of the histoaspartic protease that had a tendency to remain stable during all runs. The results have shown that hydrogen-bonded residues Ser37-His34-Asp214 are arranged without any strain, in a manner that resembles the active site of a serine protease, while Ser38 and Asn39 take up positions appropriate to formation of an oxyanion hole. Although there are several important differences between the enzyme and plasmepsin II, all of the structural features associated with a typical pepsin-like aspartic protease are present in the final model of the histoaspartic protease. A possibility that this enzyme may function as a serine protease is discussed.
authors.affiliation: V. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia. andreeva@genome.eimb.relarn.ru
authors.forename: N
authors.initials: N
authors.lastname: Andreeva
authors.forename: P
authors.initials: P
authors.lastname: Bogdanovich
authors.forename: I
authors.initials: I
authors.lastname: Kashparov
authors.forename: M
authors.initials: M
authors.lastname: Popov
authors.forename: M
authors.initials: M
authors.lastname: Stengach
mesh_terms: D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000737:chemistry; D001665:Binding Sites; D003198:Computer Simulation; D008958:Models, Molecular; D010434:Pepsin A / Q000737:chemistry*; D010963:Plasmodium falciparum / Q000201:enzymology; D017510:Protein Folding; D015800:Protozoan Proteins; D012697:Serine Endopeptidases / Q000737:chemistry* / Q000378:metabolism
chemical_list: D015800:Protozoan Proteins; D012697:Serine Endopeptidases; D016282:Aspartic Acid Endopeptidases; D010434:Pepsin A; C100637:plasmepsin II