Elucidating the role of conserved glutamates in H+-pyrophosphatase of Rhodospirillum rubrum.
H(+)-pyrophosphatase (H(+)-PPase) catalyzes pyrophosphate-driven proton transport against the electrochemical potential gradient in various biological membranes. All 50 of the known H(+)-PPase amino acid sequences contain four invariant glutamate residues. In this study, we use site-directed mutagenesis in conjunction with functional studies to determine the roles of the glutamate residues Glu(197), Glu(202), Glu(550), and Glu(649) in the H(+)-PPase of Rhodospirillum rubrum (R-PPase). All residues were replaced with Asp and Ala. The resulting eight variant R-PPases were expressed in Escherichia coli and isolated as inner membrane vesicles. All substitutions, except E202A, generated enzymes capable of PP(i) hydrolysis and PP(i)-energized proton translocation, indicating that the negative charge of Glu(202) is essential for R-PPase function. The hydrolytic activities of all other PPase variants were impaired at low Mg(2+) concentrations but were only slightly affected at high Mg(2+) concentrations, signifying that catalysis proceeds through a three-metal pathway in contrast to wild-type R-PPase, which employs both two- and three-metal pathways. Substitution of Glu(197), Glu(202), and Glu(649) resulted in decreased binding affinity for the substrate analogues aminomethylenediphosphonate and methylenediphosphonate, indicating that these residues are involved in substrate binding as ligands for bridging metal ions. Following the substitutions of Glu(550) and Glu(649), R-PPase was more susceptible to inactivation by the sulfhydryl reagent mersalyl, highlighting a role of these residues in maintaining enzyme tertiary structure. None of the substitutions affected the coupling of PP(i) hydrolysis to proton transport.
Malinen
Anssi M
AM
Department of Biochemistry and Food Chemistry, University of Turku, FIN-20014 Turku, Finland.
Belogurov
Georgiy A
GA
Salminen
Mirka
M
Baykov
Alexander A
AA
Lahti
Reijo
R
D019943:Amino Acid Substitution; D001692:Biological Transport; D017124:Conserved Sequence; D022162:Cytoplasmic Vesicles / Q000378:metabolism; D011756:Diphosphates / Q000378:metabolism; D004164:Diphosphonates / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004926:Escherichia coli / Q000166:cytology / Q000378:metabolism; D005971:Glutamates / Q000235:genetics* / Q000378:metabolism*; D006868:Hydrolysis; D043564:Inorganic Pyrophosphatase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D007700:Kinetics; D008274:Magnesium / Q000737:chemistry / Q000378:metabolism; D008634:Mersalyl / Q000494:pharmacology; D016297:Mutagenesis, Site-Directed; D011522:Protons; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012247:Rhodospirillum rubrum / Q000201:enzymology* / Q000235:genetics
D011756:Diphosphates; D004164:Diphosphonates; D004791:Enzyme Inhibitors; D005971:Glutamates; D011522:Protons; D011994:Recombinant Proteins; D008634:Mersalyl; D043564:Inorganic Pyrophosphatase; D008274:Magnesium