A subtractive approach to characterize genes with regionalized expression in the gliogenic ventral neuroepithelium: identification of chick sulfatase 1 as a new oligodendrocyte lineage gene.
To address the question of the origin of glial cells and the mechanisms leading to their specification, we have sought to identify novel genes expressed in glial progenitors. We adopted suppression subtractive hybridization (SSH) to establish a chick cDNA library enriched for genes specifically expressed at 6 days of incubation (E6) in the ventral neuroepithelium, a tissue previously shown to contain glial progenitors. Screens were then undertaken to select differentially expressed cDNAs, and out of 82 unique SSH clones, 21 were confirmed to display a regionalized expression along the dorsoventral axis of the E6 ventral neuroepithelium. Among these, we identified a transcript coding for the chick orthologue of Sulf1, a recently identified cell surface sulfatase, as a new, early marker of oligodendrocyte (OL) precursors in the chick embryonic spinal cord. This study provides groundwork for the further identification of genes involved in glial specification.
Braquart-Varnier
Christine
C
Centre de Biologie du Développement, UMR5547 CNRS/UPS, Université Paul Sabatier, 31062 Toulouse Cedex, France.
Danesin
Cathy
C
Clouscard-Martinato
Catherine
C
Agius
Eric
E
Escalas
Nathalie
N
Benazeraf
Bertrand
B
Ai
Xingbin
X
Emerson
Charles
C
Cochard
Philippe
P
Soula
Cathy
C
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D019521:Body Patterning / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics; D002490:Central Nervous System / Q000166:cytology / Q000196:embryology* / Q000201:enzymology; D002642:Chick Embryo; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D004847:Epithelial Cells / Q000166:cytology / Q000201:enzymology*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D015698:Genomic Library; D009419:Nerve Tissue Proteins / Q000235:genetics; D009693:Nucleic Acid Hybridization / Q000379:methods; D009836:Oligodendroglia / Q000166:cytology / Q000201:enzymology*; D013234:Stem Cells / Q000166:cytology / Q000201:enzymology*; D015238:Sulfotransferases / Q000235:genetics*
D015415:Biomarkers; D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; C470791:SULF1 protein, human; D015238:Sulfotransferases