title: Effect of laparoscopy versus laparotomy on circulating tumor cells using isolation by size of epithelial tumor cells.
abstract: OBJECTIVE
  To assess the effect of laparoscopy on circulating tumor cell (CTC) detection in case of carcinosis.
  
  
  METHODS
  We compared laparoscopy versus laparotomy on tumor cell blood release in an animal model of ovarian carcinosis obtained by intraperitoneal inoculation of IGR-OV1 cells in nude rats. Animals were randomly assigned to one of the following groups: CO(2) laparoscopy (L), gasless laparoscopy (GL), midline laparotomy (ML), or general anesthesia as control (C). A 0.5 ml blood sample was taken in each case before and after experiment and tested with a novel assay, ISET (isolation by size of epithelial tumor cells), which isolates CTC by filtration on account of their size. Statistics were performed with the Fisher's and the Chi-square tests.
  
  
  RESULTS
  Ten rats were included in each group. We did not find any significant difference in CTC prevalence before and after surgery (2/14 versus 3/19, respectively, P = 1). Similarly, the three surgical accesses were equivalent with one post-experiment detection per group: 1/5 for L, 1/7 for ML, 1/7 for GL, and 1/6 for C (P = 0.9).
  
  
  CONCLUSIONS
  This trial did not show any deleterious effect of laparoscopy on CTC when compared to laparotomy.
authors.affiliation: Service de Chirurgie Gynécologique et Oncologique, Hôpital Européen Georges Pompidou, 20 rue Leblanc, 75015 Paris, France. lelievre.loic@wanadoo.fr
authors.forename: L
authors.initials: L
authors.lastname: Lelievre
authors.forename: P
authors.initials: P
authors.lastname: Paterlini-Brechot
authors.forename: S
authors.initials: S
authors.lastname: Camatte
authors.forename: E
authors.initials: E
authors.lastname: Tartour
authors.forename: M
authors.initials: M
authors.lastname: Aggerbeck
authors.forename: F
authors.initials: F
authors.lastname: Vilde
authors.forename: F
authors.initials: F
authors.lastname: Lecuru
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D010535:Laparoscopy / Q000009:adverse effects*; D007813:Laparotomy / Q000009:adverse effects*; D009368:Neoplasm Transplantation; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000473:pathology / Q000601:surgery*; D011897:Random Allocation; D051381:Rats; D011923:Rats, Nude