Production and characterization of peptide mimotopes of phenolic glycolipid-I of Mycobacterium leprae.
Phenolic glycolipid-I (PGL-I), a Mycobacterium leprae-specific antigen, has been widely used for the serodiagnosis of leprosy and has been implicated in the pathogenesis of leprosy. In an effort to produce an alternate antigen of PGL-I, the mimotope peptides of PGL-I, W(T/R)LGPY(V/M), were obtained using a monoclonal antibody, III603.8, specific to PGL-I by a phage library. The biotin-labeled predominant mimotope peptide of PGLP1, WTLGPYV, bound to III603.8 in a dose-dependent manner in an immunoassay. However, PGLP1 did not bind to anti-PGL-I antibodies in the serum samples from leprosy patients that were reactive to PGL-I. Although the mimotope peptide of WTLGPYV was not effective as an alternate antigen of PGL-I for the serodiagnosis of leprosy, but it would be of interest to know how the mimotope peptides mimic the role of PGL-I antigen in the pathogenesis of M. leprae infection.
Youn
Ju Ho
JH
Department of Microbiology, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 134 Shinchon-dong, Seoul 120-752, Republic of Korea.
Myung
Han-Jeong
HJ
Liav
Abraham
A
Chatterjee
Delphi
D
Brennan
Patrick J
PJ
Choi
In-Hong
IH
Cho
Sang-Nae
SN
Shin
Jeon-Soo
JS
D020816:Amino Acid Motifs; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000276:immunology*; D006017:Glycolipids / Q000096:biosynthesis / Q000276:immunology*; D006801:Humans; D007918:Leprosy / Q000175:diagnosis / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018716:Molecular Mimicry; D009166:Mycobacterium leprae / Q000276:immunology*; D019151:Peptide Library
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D006017:Glycolipids; D019151:Peptide Library; C045169:phenolic glycolipid I, Mycobacterium leprae