Morphological and immunological characterization of immunostimulatory complexes based on glycoglycerolipids from Laminaria japonica.
Some physicochemical properties of glycoglycerolipids (monogalactosyldiacylglycerol, digalactosyldiacylglycerol and sulfoquinovosyldiacylglycerol) from the sea algae Laminaria japonica, as well as their ability to become incorporate into immunostimulating complexes (ISCOMs), used as a delivery system of microbial and tumor antigens in vesicular form, were studied. These glycolipids were found to differ essentially in fatty acid composition, unsaturation index and thermotropic behavior. The possibility of ISCOM modification by embedding the glycolipids studied instead of a phospholipid component in vesicles was shown. A preliminary research of the immunogenicity of the pore-forming protein from Yersinia pseudotuberculosis in modified (by monogalactosyldiacylglycerol) and typical (egg phosphatidylcholine) ISCOMs did not reveal a significant enhancement of immune response in comparison with that of isolated protein.
Lee
Irina A
IA
Pacific Institute of Bioorganic Chemistry FEBRAS, Vladivostok, Russia. irinali@piboc.dvo.ru
Popov
Alexander M
AM
Sanina
Nina M
NM
Kostetsky
Eduard Y
EY
Novikova
Olga D
OD
Reunov
Anatoliy V
AV
Nagorskayaand
Vera P
VP
Shnyrov
Valeriy L
VL
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000941:Antigens / Q000276:immunology; D006017:Glycolipids / Q000276:immunology* / Q000302:isolation & purification; D007114:Immunization; D007795:Laminaria / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000187:drug effects / Q000276:immunology
D000276:Adjuvants, Immunologic; D000941:Antigens; D006017:Glycolipids; C015903:glycerolglycolipids