title: Delivery of short hairpin RNA sequences by using a replication-competent avian retroviral vector.
abstract: While recent studies have demonstrated that retroviral vectors can be used to stably express short hairpin RNA (shRNA) to inhibit gene expression, these studies have utilized replication-defective retroviruses. We describe the creation of a replication-competent, Gateway-compatible retroviral vector capable of expressing shRNA that inhibits the expression of specific genes.
authors.affiliation: Laboratory of Tumor Metastasis and Angiogenesis, Van Andel Research Institute, Grand Rapids, Michigan 49503, USA.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Bromberg-White
authors.forename: Craig P
authors.initials: CP
authors.lastname: Webb
authors.forename: Veronique S
authors.initials: VS
authors.lastname: Patacsil
authors.forename: Cindy K
authors.initials: CK
authors.lastname: Miranti
authors.forename: Bart O
authors.initials: BO
authors.lastname: Williams
authors.forename: Sheri L
authors.initials: SL
authors.lastname: Holmen
mesh_terms: D000818:Animals; D001717:Birds / Q000821:virology*; D002460:Cell Line; D045744:Cell Line, Tumor; D005347:Fibroblasts; D005822:Genetic Vectors; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication
chemical_list: D034741:RNA, Small Interfering; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases