[Incorporation of glycoproteins of the Aujeszky's disease virus ( Suid herpesvirus 1) into artificial liposome membranes and their interaction with cells].
The purpose of the case study was to investigate the interplay between liposomes, containing the in-built glycoproteins of the Aujeszky disease virus (ADV, Suid herpesvirus 1) with plasmatic membranes of sensitive cells. The conditions of reconstructing the ADV glycoproteins into artificial-liposome membranes were optimized. The above liposomes (virosomes), 40 x 200 nm, were impermeable to univalent ions, which confirmed the virosome membranes were intact. The gE and gB glycoproteins (90-98% of them) were located, inside the liposome membrane with the outwards orientation of their ecto-domain fragments. Virosomes were binding with cells in the dose-dependent mode. The purified ADV virions, the ADV gB glycoproteins and heparin inhibited such binding process of virosomes with cells, which denoted the specificity of their interaction with cells. An effective internalization of cell-binding virosomes was observed at 37 degrees C. The conclusion is that the ADV glycoproteins, constructed into the liposome membranes, simulate adequately enough the viral receptor structures and that the thus obtained virosomes could be used to investigate the interplay between alpha-herpes viruses and cells.
Vrublevskaia
V V
VV
Vinokurov
M G
MG
Kholodkov
O A
OA
Kornev
A N
AN
Morenkov
O S
OS
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D011558:Herpesvirus 1, Suid / Q000378:metabolism* / Q000502:physiology; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D013696:Temperature; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014771:Virion / Q000378:metabolism; D014779:Virus Replication
D006023:Glycoproteins; D008081:Liposomes; D014759:Viral Envelope Proteins