[Immunolocalization of the signaling protein 14-3-3 of Schistosoma japonicum].
OBJECTIVE
To study the localization of the signaling protein 14-3-3 of Schistosoma japonicum (Sj14-3-3) in the parasite.


METHODS
Cercariae were collected from the infected Oncomelania hupensis for the infection of rabbits. Fifteen-day-old schistosomula and adult worms obtained from infected rabbits 15 and 42 days post-infection were used for frozen sections and indirect immunofluorescence staining with monoclonal antibody to rSj14-3-3.


RESULTS
The results showed that the Sj14-3-3 distributed mainly in the tegument, subtegument, muscle, and parenchyma of both adult worms and 15-day-old schistosomula.


CONCLUSIONS
The wide distribution and large sites of Sj14-3-3 in the parasite were clearly demonstrated, which established a significant clue for further studies of biologic actions and application of 14-3-3 protein.
Liu
Qing-zhong
QZ
Department of Pathobiology, Anhui Medical University, Hefei 230032.
Shen
Ji-long
JL
Wang
Xue-long
XL
D048948:14-3-3 Proteins; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D012549:Schistosoma japonicum / Q000378:metabolism*; D012908:Snails; D014446:Tyrosine 3-Monooxygenase / Q000276:immunology / Q000378:metabolism*
D048948:14-3-3 Proteins; D000911:Antibodies, Monoclonal; D014446:Tyrosine 3-Monooxygenase