title: 7-Nitroindazole upregulates phosphorylated cAMP response element binding protein, polysialylated-neural cell adhesion molecule and tryptophan hydroxylase expression in the adult rat hippocampus.
abstract: We examined the effect of nitric oxide (NO) on the expression of phosphorylated cAMP response element binding (pCREB) protein and on the polysialylated form of the neural cell adhesion molecule (PSA-NCAM), in the hippocampus of the adult rat, by administering 7-nitroindazole (7-NI) for 5 days, to block neuronal nitric oxide synthase (nNOS) activity. It was found that PSA-NCAM and pCREB immunoreactive cells were increased two-fold in the dentate gyrus by this treatment. In addition, the immunoreactivities of serotonin (5-HT, 5-hydroxytryptamine), known to be involved in neural plasticity, and of tryptophan hydroxylase immunoreactive-fibers in the dentate gyrus were both upregulated by 7-NI. Our results indicate that nNOS inhibition upregulates neural plasticity and increases 5-HT in the rat hippocampus, and suggest the existence of regulatory relationships between NO, neural plasticity and 5-HT.
authors.affiliation: Department of Anatomy, Medical Science and Engineering Research Center for Reactive Oxygen Species, College of Medicine, Kyunghee University, Hoeki-Dong 1, Dongdaemun-Gu, Seoul 130-701, South Korea.
authors.forename: Chan
authors.initials: C
authors.lastname: Park
authors.forename: Keumok
authors.initials: K
authors.lastname: Cho
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Ryu
authors.forename: Ki Soon
authors.initials: KS
authors.lastname: Shin
authors.forename: Junghye
authors.initials: J
authors.lastname: Kim
authors.forename: Heekyung
authors.initials: H
authors.lastname: Ahn
authors.forename: Youngbuhm
authors.initials: Y
authors.lastname: Huh
mesh_terms: D000818:Animals; D002452:Cell Count / Q000379:methods; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D007191:Indazoles / Q000494:pharmacology*; D008297:Male; D039842:Neural Cell Adhesion Molecule L1 / Q000378:metabolism*; D011903:Raphe Nuclei / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012794:Sialic Acids / Q000378:metabolism*; D014365:Tryptophan Hydroxylase / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D004791:Enzyme Inhibitors; D007191:Indazoles; D039842:Neural Cell Adhesion Molecule L1; D012794:Sialic Acids; C103469:polysialyl neural cell adhesion molecule; D014365:Tryptophan Hydroxylase; C080122:7-nitroindazole