Inability of simian virus 40 to establish productive infection of lymphoblastic cell lines.
Lymphoblastic cell lines were infected with simian virus 40 (SV40) and then monitored for evidence of a productive infection. No evidence of early gene expression was found 2 days following infection, as determined by assaying viral mRNAs and early antigens. Furthermore, only small amounts of virus could be detected by plaque assay 2 days after infection, and levels slowly declined until they were undetectable after a few weeks in culture. Thus, human lymphocytes are not readily infectible with SV40 and do not provide a simple model for studying interactions of SV40 with a human cell type.
Shaikh
Sophie
S
Department of Microbiology-Immunology, Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, Illinois 60611, USA.
Skoczylas
Christine
C
Longnecker
Richard
R
Rundell
Kathleen
K
D002460:Cell Line; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D006801:Humans; D008214:Lymphocytes / Q000821:virology*; D013539:Simian virus 40 / Q000472:pathogenicity*; D010948:Viral Plaque Assay