Specific regulation of SOD isoforms by NaCl and osmotic stress in leaves of the C3 halophyte Suaeda salsa L.
The halophyte Suaeda salsa L., exposed to different NaCl concentrations (100 and 400 mmol/L) and polyethylene glycol (isoosomotic to 100 mmol/L NaCl) containing nutrient solutions under normal or K+-deficient conditions for 7 days, was used to study effects of NaCl salinity and osmotic stress on chlorophyll content, chlorophyll fluorescence characteristics, malonedialdehyde (MDA) content, and superoxide dismutase (SOD) isoform activities. Photosynthetic capacity was not decreased by NaCl treatment, indicating that S. salsa possesses an effective antioxidative response system for avoiding oxidative damage. Seven SOD activity bands were detected in S. salsa leaf extracts, including an Mn-SOD and several isoforms of Fe-SOD and CuZn-SOD. It turned out that NaCl salinity and osmotic stress lead to a differential regulation of distinct SOD isoenzymes. This differential regulation is suggested to play a major role in stress tolerance of S. salsa.
Wang
Baoshan
B
Institut für Botanik, Technische Universität Darmstadt, Fachbereich 10, Schnittspahnstrasse 3-5, D-64287 Darmstadt, Germany.
Lüttge
Ulrich
U
Ratajczak
Rafael
R
D019663:Chenopodiaceae / Q000187:drug effects* / Q000201:enzymology* / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D005453:Fluorescence; D007527:Isoenzymes / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009997:Osmotic Pressure; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism*
D007527:Isoenzymes; D002734:Chlorophyll; D012965:Sodium Chloride; D008315:Malondialdehyde; D013482:Superoxide Dismutase