[Preliminary study on proteomic patterns in hepatic tissue to identify HBV related hepatocellular carcinoma].
OBJECTIVE
To identify proteomic patterns in hepatic tissues for diagnosing early HBV related HCC.


METHODS
Proteomic spectra were generated by two-dimensional gel electrophoresis (2-DE), A preliminary "raining" set of spectra derived from analysis of 14 cancer tissues and 14 non-cancer tissues, a proteomic patterns that completely discriminated cancer from non-cancer was identified. The discovered pattern was then used to classify an independent set of 48 masked samples: 24 from cancer tissues, and 24 from non-cancer tissues.


RESULTS
The discriminatory pattern correctly identified all cancer tissues and non-cancer tissues in the masked set. This result yielded a sensitivity of 100%, specificity of 100%.


CONCLUSIONS
Further analysis on these proteins in the proteomic pattern will be helpful to screen tumor markers for HBV related HCC. These findings justify a prospective assessment of proteomic pattern technology as a screening tool for cancer in high-risk and general populations.
Long
Yun-zhu
YZ
Department of Infectious Diseases, XiangYa Hospital, Central South University, Changsha 410008, China.
Fan
Xue-gong
XG
Li
Ning
N
Huang
Yu-kun
YK
D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000209:etiology; D006509:Hepatitis B / Q000150:complications*; D006801:Humans; D008099:Liver / Q000737:chemistry*; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000209:etiology; D009363:Neoplasm Proteins / Q000032:analysis; D040901:Proteomics
D009363:Neoplasm Proteins