The evolution of microarrayed compound screening.
This review describes recent developments in the evolutionary process of microarrayed compound screening (microARCS to become a robust and efficient ultra-high-throughput screening technology. Improvements in compound spotting (including new quality-control methods), gel casting and imaging, together with software capable of automatic analysis and deconvolution of images, have helped to streamline the screening process. A variety of screening projects using cell-based and non-cell-based approaches have been successfully concluded using microARCS. Comparison of hits derived from standard microtitre-plate-based screening and from microARCS reveals excellent overlap. Furthermore, there seems to be no bias towards finding compounds within a particular range of logP values, even though compounds are solubilized from a dry state during the course of the assay.
Hoever
Michael
M
Discovery Partners International AG, Gewerbestrasse 16, CH-4123 Allschwil, Switzerland. mhoever@discoverypartners.com
Zbinden
Peter
P
D001681:Biological Assay / Q000379:methods; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005782:Gels; D008904:Miniaturization / Q000295:instrumentation / Q000379:methods; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D012984:Software; D013329:Structure-Activity Relationship
D005782:Gels; D004364:Pharmaceutical Preparations