Intratracheal application of recombinant surfactant protein-C surfactant to rabbits attenuates acute lung injury induced by intratracheal acidified infant formula.
Our aim in the current study was to determine whether recombinant surfactant protein-C (rSP-C) surfactant improves acute lung injury (ALI) induced by intratracheal acidified milk products. Twenty-eight rabbits were randomly divided into four groups. ALI was induced with intratracheal acidified infant formula (0.8 mL/kg, pH 1.8) in 3 groups. The control group received intratracheal acidified saline. Therapy groups received 1 of 2 doses of intratracheal rSP-C surfactant (0.5 or 2 SP-C mg/kg) 30 min after the acidified infant formula. The lungs were ventilated with 100% oxygen for 4 h after induction of ALI. Acidified infant formula dramatically reduced oxygenation and lung compliance, and increased resistance. Both doses of rSP-C improved the variables [mean PaO(2) (mm Hg) and compliance (mL/cm H(2)O) at 4 h: 61 and 0.4 for infant formula, 162 and 1.0 for small-dose rSP-C, and 152 and 1.2 for large-dose rSP-C, respectively; P < 0.05]. Pulmonary leukosequestration and edema, and severe morphological changes were attenuated by rSP-C treatment (ALI score: 14, 7, 7 in infant formula, small-dose rSP-C, and large-dose rSP-C; P < 0.05). The efficacy was similar for the two doses of rSP-C. These findings suggest that intratracheal administration of rSP-C ameliorates ALI induced by aspiration of acidified milk products.


CONCLUSIONS
Small or large doses of recombinant surfactant protein-C surfactant given 30 min after intratracheal acidified infant formula attenuated physiological, biochemical, and morphological lung damage.
Mikawa
Katsuya
K
Department of Anesthesia and Perioperative Medicine, Kobe University Graduate School of Medicine, Kobe, Japan. katzmikawa@yahoo.co.jp
Nishina
Kahoru
K
Takao
Yumiko
Y
Obara
Hidefumi
H
D000403:Airway Resistance / Q000187:drug effects / Q000502:physiology; D000818:Animals; D001784:Blood Gas Analysis; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D006339:Heart Rate / Q000187:drug effects; D006439:Hemodynamics / Q000502:physiology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007223:Infant; D007225:Infant Food; D007442:Intubation, Intratracheal; D007958:Leukocyte Count; D008163:Luminescent Measurements; D008168:Lung / Q000473:pathology / Q000503:physiopathology; D055370:Lung Injury; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D010976:Platelet Count; D011015:Pneumonia, Aspiration / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D011652:Pulmonary Circulation / Q000187:drug effects / Q000502:physiology; D037721:Pulmonary Surfactant-Associated Protein C / Q000008:administration & dosage / Q000627:therapeutic use*; D011817:Rabbits; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000627:therapeutic use; D015656:Respiratory Mechanics / Q000187:drug effects / Q000502:physiology
D037721:Pulmonary Surfactant-Associated Protein C; D011994:Recombinant Proteins