Studies on intestinal permeability of cirrhotic patients by analysis lactulose and mannitol in urine with HPLC/RID/MS.
The method for separation and determination of lactulose (L) and mannitol (M) in urine was developed by HPLC with a refractive index detector (RID). The linearity ranged from 5 to 1000 microg/mL for L and M, respectively. Recoveries ranged from 93.1% to 97.1%. The intra- and inter-day relative standard deviations of peak area were between 0.8-1.4% (n=3) and 1.4-3.6% (n=3). The limits of detection were obtained with 1.40 microg/mL for L and 1.65 microg/mL for M. The ratios of L/M in the urine samples for the spontaneous ascitic fluid infection (SAI), sterile ascitic fluid (SA) patients, and healthy volunteers (HV) were determined. The results showed well the correlations among the L/M ratio, intestinal permeability (IP) and the illness status of patients, and also indicated lactulose could improve the IP of SAI patients. The peaks of L and M in chromatograms were identified by electrospray ionization/mass spectrometry (ESI/MS), which ensured the accurate measurement of the ratio L/M. This method presented a rapid, accurate, and practical technique for determining IP in clinical practice and investigating the pathology of hepatocirrhosis.
Liu
Hongxia
H
Chemistry Department, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, No. 75 Daxue Road, Zhengzhou 450052, PR China. liuhongxia999@371.net
Zhang
Shusheng
S
Yu
Ajuan
A
Qu
Lingbo
L
Zhao
Yufen
Y
Huang
Hongchun
H
Li
Jichang
J
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007408:Intestinal Absorption; D007792:Lactulose / Q000652:urine*; D008103:Liver Cirrhosis / Q000503:physiopathology*; D008353:Mannitol / Q000652:urine*; D010539:Permeability; D012031:Refractometry; D021241:Spectrometry, Mass, Electrospray Ionization
D008353:Mannitol; D007792:Lactulose