title: In vitro assays for kinetoplastid U insertion-deletion editing and associated activities.
abstract: This chapter describes biochemical assays that have been used in analyzing RNA editing in kinetoplastid mitochondria and to characterize the general mechanism of editing by the editosome. Studies using these assays have shown that the characteristics of each activity contribute to editing site selection, U addition and removal, and RNA ligation resulting in accurately edited mRNAs.
authors.affiliation: Seattle Biomedical Research Institute, and Department of Pathobiology, University of Washington, Seattle, USA.
authors.forename: Kenneth
authors.initials: K
authors.lastname: Stuart
authors.forename: Reza
authors.initials: R
authors.lastname: Salavati
authors.forename: Robert P
authors.initials: RP
authors.lastname: Igo
authors.forename: Nancy
authors.initials: N
authors.lastname: Lewis Ernst
authors.forename: Setareh S
authors.initials: SS
authors.lastname: Palazzo
authors.forename: Bingbing
authors.initials: B
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D016830:Kinetoplastida / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction / Q000379:methods; D017393:RNA Editing / Q000235:genetics*; D016337:RNA, Catalytic / Q000235:genetics; D016053:RNA, Protozoan / Q000235:genetics; D017384:Sequence Deletion; D014498:Uracil
chemical_list: D016337:RNA, Catalytic; D016053:RNA, Protozoan; C427204:hammerhead ribozyme; D014498:Uracil