[Small but high throughput: how "tissue-microarrays" became a favorite tool for pathologists and scientists].
Progress in the knowledge of molecular genetics and availability of high-throughput technologies offer the opportunity to identify new diagnostic and prognostic markers and new therapeutic targets in human cancer. The recently developed "tIssue microarrays" (TMA) technology allows parallel molecular profiling of clinical samples. Using this technique and immunohistochemistry (IHC), fluorescence in situ hybridisation (FISH), or RNA in situ hybridisation (ISH), the pathologist is now able to perform unprecedented large-scale analyses. The advantages are significant: large number of cases assessed simultaneously for numerous markers, processed in identical conditions, from reduced amount of archival tIssues, with an excellent correlation with standard methods, and a reduction in cost and time. This Article provides a short review of this technology, and points out several aspects of the TMA construction and its applications for clinical research.
Jacquemier
Jocelyne
J
Département d'Oncologie Moléculaire, Institut Paoli-Calmettes, 232 Bd de Sainte Marguerite, 13273 Marseille Cedex 9, France. jacquemierj@marseille.Fnclcc.fr
Ginestier
Christophe
C
Charafe-Jauffret
Emmanuelle
E
Bertucci
François
F
Bege
Thierry
T
Geneix
Jeanine
J
Birnbaum
Daniel
D
D015415:Biomarkers / Q000032:analysis*; D003362:Cost-Benefit Analysis; D020869:Gene Expression Profiling; D023281:Genomics; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D017404:In Situ Hybridization, Fluorescence; D009369:Neoplasms / Q000737:chemistry / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis / Q000191:economics / Q000592:standards; D010336:Pathology / Q000379:methods* / Q000639:trends; D010597:Pharmacogenetics / Q000379:methods; D040081:Protein Array Analysis / Q000191:economics / Q000592:standards; D040901:Proteomics; D011786:Quality Control; D012106:Research / Q000639:trends; D012107:Research Design; D013048:Specimen Handling
D015415:Biomarkers