A novel calmodulin-binding protein functions as a negative regulator of osmotic stress tolerance in Arabidopsis thaliana seedlings.
A clone for a novel Arabidopsisthaliana calmodulin (CaM)-binding protein of 25 kDa (AtCaMBP25) has been isolated by using a radiolabelled CaM probe to screen a cDNA expression library derived from A. thaliana cell suspension cultures challenged with osmotic stress. The deduced amino acid sequence of AtCaMBP25 contains putative nuclear localization sequences and shares significant degree of similarity with hypothetical plant proteins only. Fusion of the AtCaMBP25 coding sequence to reporter genes targets the hybrid protein to the nucleus. Bacterially expressed AtCaMBP25 binds, in a calcium-dependent manner, to a canonical CaM but not to a less conserved isoform of the calcium sensor. AtCaMBP25 is encoded by a single-copy gene, whose expression is induced in Arabidopsis seedlings exposed to dehydration, low temperature or high salinity. Transgenic plants overexpressing AtCaMBP25 exhibits an increased sensitivity to both ionic (NaCl) and non-ionic (mannitol) osmotic stress during seed germination and seedling growth. By contrast, transgenic lines expressing antisense AtCaMBP25 are significantly more tolerant to mannitol and NaCl stresses than the wild type. Thus, the AtCaMBP25 gene functions as a negative effector of osmotic stress tolerance and likely participates in stress signal transduction pathways.
Perruc
Elian
E
Surfaces cellulaires et signalisation chez les végétaux, UMR 5546 CNRS/Université Paul Sabatier, Pôle de Biotechnologie Végétale, BP 17 Auzeville, 31326 Castanet-Tolosan Cedex, France.
Charpenteau
Martine
M
Ramirez
Bertha Cecilia
BC
Jauneau
Alain
A
Galaud
Jean-Philippe
JP
Ranjeva
Raoul
R
Ranty
Benoît
B
D000222:Adaptation, Physiological / Q000235:genetics* / Q000502:physiology; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002148:Calmodulin-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008353:Mannitol / Q000494:pharmacology; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals / Q000235:genetics; D009997:Osmotic Pressure / Q000187:drug effects; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology
D029681:Arabidopsis Proteins; D002148:Calmodulin-Binding Proteins; D019913:Nuclear Localization Signals; C486918:calmodulin-binding protein 25 kDa, Arabidopsis; D008353:Mannitol; D012965:Sodium Chloride; D002118:Calcium