title: Tuber borchii fruit body: 2-dimensional profile and protein identification.
abstract: The formation of the fruit body represents the final phase of the ectomycorrhizal fungus T. borchii life cycle. Very little is known concerning the molecular and biochemical processes involved in the fructification phase. 2-DE maps of unripe and ripe ascocarps revealed different protein expression levels and the comparison of the electropherograms led to the identification of specific proteins for each developmental phase. Associating micropreparative 2-DE to microchemical approaches, such as N-terminal sequencing and 2-D gel-electrophoresis mass-spectrometry, proteins playing pivotal roles in truffle physiology were identified.
authors.affiliation: Istituto di Chimica Biologica Giorgio Fornaini, Università degli Studi di Urbino Carlo Bo, Via A. Saffi, 2, I-61029 Urbino (PU), Italy.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Pierleoni
authors.forename: Michele
authors.initials: M
authors.lastname: Buffalini
authors.forename: Luciana
authors.initials: L
authors.lastname: Vallorani
authors.forename: Chiara
authors.initials: C
authors.lastname: Guidi
authors.forename: Sabrina
authors.initials: S
authors.lastname: Zeppa
authors.forename: Cinzia
authors.initials: C
authors.lastname: Sacconi
authors.forename: Piero
authors.initials: P
authors.lastname: Pucci
authors.forename: Angela
authors.initials: A
authors.lastname: Amoresano
authors.forename: Annarita
authors.initials: A
authors.lastname: Casbarra
authors.forename: Vilberto
authors.initials: V
authors.lastname: Stocchi
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D020539:Sequence Analysis, Protein / Q000379:methods; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D005656:Fungal Proteins