Spatiotemporal patterns of Musashi1 expression during inner ear development.
Musashi1 (Msi 1) is an RNA binding protein associated with asymmetric cell divisions in neural progenitor cells. To investigate the involvement of Msi1 in the inner ear development, we studied the expression of Msi1 in mouse inner ears with RT-PCR and immunohistochemistry. Immunohistochemistry revealed that Msi1 was expressed in all otocyst cells at embryonic day (E) 10 and 12. Msi1 immunoreactivity became lost in hair cells after E14 in vestibule and after E16 in cochlea, whereas it persisted in supporting cells until adulthood. The subcellular localization of Msi1 changed from "cytoplasmic predominance" to "nuclear predominance" during the first 2 weeks after birth. The present data suggested that Msi may play a role in inner ear development.
Sakaguchi
Hirofumi
H
Department of Pathology and Applied Neurobiology, Kyoto Prefectural University of Medicine Graduate School of Medical Science, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.
Yaoi
Takeshi
T
Suzuki
Toshihiro
T
Okano
Hideyuki
H
Hisa
Yasuo
Y
Fushiki
Shinji
S
D000818:Animals; D007758:Ear, Inner / Q000737:chemistry / Q000196:embryology* / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000096:biosynthesis*; D011247:Pregnancy; D016601:RNA-Binding Proteins / Q000032:analysis / Q000096:biosynthesis*; D013997:Time Factors
C104786:Msi1h protein, mouse; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins