title: The development of larger cells that spontaneously escape senescence--a step during the immortalization of a human cancer cell line.
abstract: There are few information concerning the changes associated with the transition interval when slow growing, primary explanted human cancer cells are displaced by new selected faster growing cells and became an immortal cell line. In a previous paper (J. Cell. Mol. Med., 5: 49-59, 2001) we described the TV cell line derived from a laryngeal tumor which harbors human papillomavirus (HPV) gene sequences throughout more than sixty in vitro passages. In this paper we analyze the modifications observed during the crisis interval when significant amount of cells senesce but occasional cells acquire some mutations that make them immortal. Confocal microscopy analysis revealed the heterogeneity of the cells in terms of their size and nucleus/cell ratio. Proliferation capacity was assessed by flow cytometry analyzing DNA content and expression of transferrin receptor (CD71). We discussed the possibility that HPV genome sequences alleviate a proliferation block during the crisis growth arrest of human larynx carcinoma cell line and the possibility that the cells monitor their size and growth by measuring the levels of some protein whose synthesis is coupled to cell development.
authors.affiliation: St. S. Nicolau Institute of Virology, Bucharest, Romania. ccdiaconu@yahoo.com
authors.forename: Carmen C
authors.initials: CC
authors.lastname: Diaconu
authors.forename: Coralia
authors.initials: C
authors.lastname: Bleotu
authors.forename: Mihaela
authors.initials: M
authors.lastname: Chivu
authors.forename: Irina
authors.initials: I
authors.lastname: Alexiu
authors.forename: Daniela
authors.initials: D
authors.lastname: Petrusca
authors.forename: Gabriela
authors.initials: G
authors.lastname: Anton
authors.forename: Ruxandra
authors.initials: R
authors.lastname: Achim
authors.forename: Simona M
authors.initials: SM
authors.lastname: Ruta
authors.forename: C
authors.initials: C
authors.lastname: Cernescu
mesh_terms: D015703:Antigens, CD / Q000096:biosynthesis; D000944:Antigens, Differentiation, B-Lymphocyte / Q000096:biosynthesis; D002277:Carcinoma / Q000473:pathology; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D002460:Cell Line; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D016922:Cellular Senescence; D004247:DNA / Q000737:chemistry; D005434:Flow Cytometry; D016679:Genome, Viral; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology; D018613:Microscopy, Confocal; D009154:Mutation; D027383:Papillomaviridae / Q000235:genetics; D011003:Ploidies; D016133:Polymerase Chain Reaction; D011990:Receptors, Transferrin / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; C068126:CD71 antigen; D011990:Receptors, Transferrin; D004247:DNA