Expression analyses of zebrafish transferrin, ifabp, and elastaseB mRNAs as differentiation markers for the three major endodermal organs: liver, intestine, and exocrine pancreas.
In the present work, three zebrafish cDNA clones encoding transferrin, intestinal fatty acid binding protein (IFABP), and elastaseB were cloned and their expression patterns in early zebrafish development were characterized as differentiation markers for the three major endoderm organs: liver, intestine, and exocrine pancreas. transferrin and ifabp mRNAs exhibit a biphasic expression pattern during early development. transferrin mRNAs were first expressed at approximately 7 hours postfertilization (hpf) in the yolk syncytial layer (YSL) and later in the liver rudiment (from approximately 48 hpf) and in the esophagus transiently (72-96 hpf). Ifabp mRNAs were initially expressed in the YSL at the ventral side during late epiboly (8-9 hpf), spread throughout the YSL of later stage embryos, and appeared in the intestine rudiment at approximately 36 hpf. In contrast to the transferrin and ifabp mRNAs, elastaseB mRNAs were not expressed in the yolk sac or YSL, and these transcripts were detected exclusively in the exocrine pancreas after approximately 56 hpf.
Mudumana
Sudha Puttur
SP
Department of Biological Sciences, National University of Singapore, Singapore.
Wan
Haiyan
H
Singh
Malkeet
M
Korzh
Vladimir
V
Gong
Zhiyuan
Z
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002347:Carps; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D007413:Intestinal Mucosa / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D010179:Pancreas / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000096:biosynthesis* / Q000235:genetics; D013997:Time Factors; D014168:Transferrin / Q000096:biosynthesis* / Q000235:genetics; D015017:Yolk Sac / Q000378:metabolism; D015027:Zebrafish
D002352:Carrier Proteins; D018076:DNA, Complementary; D050556:Fatty Acid-Binding Proteins; D012333:RNA, Messenger; D014168:Transferrin; D007501:Iron; D012697:Serine Endopeptidases; C117309:pancreatic elastase II