title: The bchU gene of Chlorobium tepidum encodes the c-20 methyltransferase in bacteriochlorophyll c biosynthesis.
abstract: Bacteriochlorophylls (BChls) c and d, two of the major light-harvesting pigments in photosynthetic green sulfur bacteria, differ only by the presence of a methyl group at the C-20 methine bridge position in BChl c. A gene potentially encoding the C-20 methyltransferase, bchU, was identified by comparative analysis of the Chlorobium tepidum and Chloroflexus aurantiacus genome sequences. Homologs of this gene were amplified and sequenced from Chlorobium phaeobacteroides strain 1549, Chlorobium vibrioforme strain 8327d, and C. vibrioforme strain 8327c, which produce BChls e, d, and c, respectively. A single nucleotide insertion in the bchU gene of C. vibrioforme strain 8327d was found to cause a premature, in-frame stop codon and thus the formation of a truncated, nonfunctional gene product. The spontaneous mutant of this strain that produces BChl c (strain 8327c) has a second frameshift mutation that restores the correct reading frame in bchU. The bchU gene was inactivated in C. tepidum, a BChl c-producing species, and the resulting mutant produced only BChl d. Growth rate measurements showed that BChl c- and d-producing strains of the same organism (C. tepidum or C. vibrioforme) have similar growth rates at high and intermediate light intensities but that strains producing BChl c grow faster than those with BChl d at low light intensities. Thus, the bchU gene encodes the C-20 methyltransferase for BChl c biosynthesis in Chlorobium species, and methylation at the C-20 position to produce BChl c rather than BChl d confers a significant competitive advantage to green sulfur bacteria living at limiting red and near-infrared light intensities.
authors.affiliation: Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: Julia A
authors.initials: JA
authors.lastname: Maresca
authors.forename: Aline
authors.initials: A
authors.lastname: Gomez Maqueo Chew
authors.forename: Marta Ros
authors.initials: MR
authors.lastname: Ponsatí
authors.forename: Niels-Ulrik
authors.initials: NU
authors.lastname: Frigaard
authors.forename: John G
authors.initials: JG
authors.lastname: Ormerod
authors.forename: Donald A
authors.initials: DA
authors.lastname: Bryant
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis*; D001429:Bacteriochlorophylls / Q000096:biosynthesis*; D041883:Chlorobium / Q000235:genetics* / Q000254:growth & development; D005798:Genes, Bacterial; D008780:Methyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D010860:Pigments, Biological / Q000032:analysis
chemical_list: D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D010860:Pigments, Biological; C002084:bacteriochlorophyll c; D008780:Methyltransferases