title: Effect of phytase supplementation on phosphorus digestibility in low-phytate barley fed to finishing pigs.
abstract: Forty crossbred barrows (Camborough 15 Line female x Canabred sire) weighing an average of 79.6 +/- 8.0 kg were used in a factorial design experiment (5 barleys x 2 enzyme levels) conducted to determine the effects of phytase supplementation on nutrient digestibility in low-phytate barleys fed to finishing pigs. The pigs were assigned to one of 10 dietary treatments comprised of a normal 2-rowed, hulled variety of barley (CDC Fleet, 0.26% phytate) or 2 low-phytate hulled genotypes designated as LP422 (0.14% phytate) and LP635 (0.09% phytate). A normal, hulless barley (CDC Dawn, 0.26% phytate) and a hulless genotype designated as LP422H (0.14% phytate) were also included. All barleys were fed with and without phytase (Natuphos 5000 FTU/kg). The diets fed contained 98% barley, 0.5% vitamin premix, 0.5% trace mineral premix, 0.5% NaCl and 0.5% chromic oxide but no supplemental phosphorus. The marked feed was provided for a 7-day acclimatization period, followed by a 3-day faecal collection. In the absence of phytase, phosphorus digestibility increased substantially (P < 0.05) as the level of phytate in the barley declined. For the hulled varieties, phosphorus digestibility increased from 12.9% for the normal barley (0.26% phytate) to 35.3 and 39.8% for the two low-phytate genotypes (0.14 and 0.09% phytate respectively). For the hulless varieties, phosphorus digestibility increased from 9.2% for the normal barley (0.26% phytate) to 34.7% for the hulless variety with 54% of the normal level of phytate (0.14% phytate). In contrast, when phytase was added to the diet, there was little difference in phosphorus digestibility between pigs fed normal barley and those fed the low-phytate genotypes (significant barley x enzyme interaction, P = 0.01). For the hulled varieties, phosphorus digestibility was 50.1% for the barley with the normal level of phytate (0.26% phytate) compared with 51.1 and 52.4% for the varieties with 54 and 35% of the normal level of phytate (0.14 and 0.09% phytate respectively). For the hulless varieties, phosphorus digestibility increased from 47.1% for the normal barley (0.26% phytate) to 54.4% for the hulless variety with 54% of the normal level of phytate (0.14% phytate). In conclusion, both supplementation with phytase and selection for low-phytate genotypes of barley were successful in increasing the digestibility of phosphorus for pigs. Unfortunately, the effects did not appear to be additive. Whether or not swine producers will choose low-phytate barley or supplementation with phytase as a means to improve phosphorus utilization, will likely depend on the yield potential of low-phytate barley and the additional costs associated with supplementation with phytase.
authors.affiliation: Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. thacker@admin.usask.ca
authors.forename: P A
authors.initials: PA
authors.lastname: Thacker
authors.forename: B G
authors.initials: BG
authors.lastname: Rossnagel
authors.forename: V
authors.initials: V
authors.lastname: Raboy
mesh_terms: D010832:6-Phytase / Q000008:administration & dosage* / Q000378:metabolism; D000821:Animal Feed; D000818:Animals; D019587:Dietary Supplements; D004063:Digestion / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D001467:Hordeum / Q000737:chemistry / Q000235:genetics; D008297:Male; D010758:Phosphorus / Q000378:metabolism*; D010833:Phytic Acid / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011897:Random Allocation; D013552:Swine / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D010758:Phosphorus; D010833:Phytic Acid; D010832:6-Phytase