title: Cadmium-induced changes in antioxidant enzymes in suspension culture of soybean cells.
abstract: Cadmium (Cd), similarly to other heavy metals, inhibits plant growth. We have recently showed that Cd(2+) either stimulates (1-4 microM) or inhibits (>or= 6 microM) growth of soybean (Glycine max L.) cells in suspension culture (Sobkowiak & Deckert, 2003, Plant Physiol Biochem. 41: 767-72). Here, soybean cell suspension cultures were treated with various concentrations of Cd(2+) (1-10 microM) and the following enzymes were analyzed by native electrophoresis: superoxide dismutase (SOD), catalase (CAT), peroxidase (POX) and ascorbate peroxidase (APOX). We found a significant correlation between the cadmium-induced changes of soybean cell culture growth and the isoenzyme pattern of the antioxidant enzymes. The results suggest that inhibition of growth and modification of antioxidant defense reactions appear in soybean cells when Cd(2+) concentration in culture medium increases only slightly, from 4 to 6 microM.
authors.affiliation: Laboratory of Plant Ecophysiology, Faculty of Biology, Adam Mickiewicz University, 61-713 Poznań, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Sobkowiak
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Rymer
authors.forename: Renata
authors.initials: R
authors.lastname: Rucińska
authors.forename: Joanna
authors.initials: J
authors.lastname: Deckert
mesh_terms: D060387:Ascorbate Peroxidases; D002104:Cadmium / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism*; D018929:Cell Culture Techniques; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D009195:Peroxidase / Q000378:metabolism*; D010544:Peroxidases / Q000494:pharmacology*; D013025:Glycine max / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D002104:Cadmium; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase