title: Sequence analysis of cDNA encoding follicle-stimulating hormone and luteinizing hormone beta-subunits in the Mongolian gerbil (Meriones unguiculatus).
abstract: To examine the molecular basis for efficient superovulation in the Mongolian gerbil, the cDNA sequences of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) beta-subunits were determined and compared with those of other mammals. FSHbeta and LHbeta cDNAs were 1637 and 507bp long, respectively, from the 5'-end to putative polyA sites. The deduced sequences of the FSHbeta and LHbeta precursor proteins were 129 and 141 amino acids in length, respectively. The amino acid sequences of both Mongolian gerbil hormone subunits showed overall similarity to those of other rodents, confirming that the combination of equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) should be effective for induction of superovulation in Mongolian gerbils, as in mice and rats. However, the use of hCG might need to be re-evaluated owing to its low homology to rodent LH.
authors.affiliation: Department of Veterinary Science, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
authors.forename: Minako
authors.initials: M
authors.lastname: Koura
authors.forename: Hiroko
authors.initials: H
authors.lastname: Handa
authors.forename: Yoko
authors.initials: Y
authors.lastname: Noguchi
authors.forename: Kaoru
authors.initials: K
authors.lastname: Takano
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Junichiro
authors.initials: J
authors.lastname: Matsuda
authors.forename: Osamu
authors.initials: O
authors.lastname: Suzuki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D037201:Follicle Stimulating Hormone, beta Subunit / Q000235:genetics*; D015723:Gene Library; D005849:Gerbillinae / Q000235:genetics*; D037101:Luteinizing Hormone, beta Subunit / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D010902:Pituitary Gland / Q000502:physiology*; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D037201:Follicle Stimulating Hormone, beta Subunit; D037101:Luteinizing Hormone, beta Subunit; D012313:RNA