title: Spatiotemporal patterns of Musashi1 expression during inner ear development.
abstract: Musashi1 (Msi 1) is an RNA binding protein associated with asymmetric cell divisions in neural progenitor cells. To investigate the involvement of Msi1 in the inner ear development, we studied the expression of Msi1 in mouse inner ears with RT-PCR and immunohistochemistry. Immunohistochemistry revealed that Msi1 was expressed in all otocyst cells at embryonic day (E) 10 and 12. Msi1 immunoreactivity became lost in hair cells after E14 in vestibule and after E16 in cochlea, whereas it persisted in supporting cells until adulthood. The subcellular localization of Msi1 changed from "cytoplasmic predominance" to "nuclear predominance" during the first 2 weeks after birth. The present data suggested that Msi may play a role in inner ear development.
authors.affiliation: Department of Pathology and Applied Neurobiology, Kyoto Prefectural University of Medicine Graduate School of Medical Science, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Sakaguchi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yaoi
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Suzuki
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Hisa
authors.forename: Shinji
authors.initials: S
authors.lastname: Fushiki
mesh_terms: D000818:Animals; D007758:Ear, Inner / Q000737:chemistry / Q000196:embryology* / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000096:biosynthesis*; D011247:Pregnancy; D016601:RNA-Binding Proteins / Q000032:analysis / Q000096:biosynthesis*; D013997:Time Factors
chemical_list: C104786:Msi1h protein, mouse; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins