Development of multiplex DNA electronic microarrays using a universal adaptor system for detection of single nucleotide polymorphisms.
The NanoChip electronic microarray is designed for the rapid detection of genetic variation in research and clinical diagnosis. We have developed a multiplex electronic microarray assay, specific for single nucleotide polymorphism (SNP) genotyping and mutation detection, using universal adaptor sequences tailed to the 5' end of PCR primers specific to each target. PCR products, amplified by primers directed to the universal adaptor sequence, are immobilized on the microarray either directly or via capture oligonucleotides complementary to the universal adaptor sequence. This simple modification results in a significant increase in fidelity with improved specificity and accuracy. In addition, the multiplexing of genetic variant detection allows increased throughput and significantly reduced cost per assay. This general schema can also be applied to other microarray and macroarray formats.
Tsang
Shirley
S
SAIC-Frederick, National Cancer Institute, Frederick, MD, USA. shirleytsang@ncifcrf.gov
Sun
Zhonghe
Z
Stewart
Claudia
C
Lum
Nicole
N
Frankenberger
Casey
C
Subleski
Marianne
M
Rasmussen
Lynn
L
Munroe
David J
DJ
D000818:Animals; D015342:DNA Probes; D051379:Mice; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide
D015342:DNA Probes