[Application of PCR to Haemophilus influenzae typing. I. PCR standardization for bexA gene fragment detection and for detection of DNA fragment specific for H.influenzae type B].
PCR standardization was performed in order to detect a fragment of bexA gene, which is presented in all capsulate H. influenzae isolates, and a DNA fragment specific for H. influenzae type b. Implementing of such PCR into H. influenzae typing may be very useful in cases of isolates from clinical material for which serotyping alone gives unclear results. Standardization of PCR detecting DNA fragments specific for all capsular types will enable to perform complete typing of H. influenzae isolates.
Zasada
Aleksandra A
AA
Zakład Bakteriologii Państwowego Zakładu Higieny w Warszawie.
Paciorek
Jarosław
J
D015373:Bacterial Typing Techniques / Q000592:standards*; D004269:DNA, Bacterial / Q000032:analysis; D019741:Haemophilus influenzae type b / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000592:standards*
D004269:DNA, Bacterial