title: Elastase and elastase inhibitor from Aspergillus fumigatus, Aspergillus flavus and Aspergillus niger.
abstract: Elastolytic and elastase inhibitory activities were investigated for 13 strains of Aspergillus fumigatus, three strains of Aspergillus flavus and three strains of Aspergillus niger. Nine of the 13 strains of A. fumigatus and all strains of A. flavus demonstrated elastase activity (more than 1 unit ml(-1)). Six of the 13 strains of A. fumigatus and all strains of A. flavus expressed elastase inhibitory activity (more than 2 units ml(-1)). However, no elastase or elastase inhibitory activities were observed with A. niger. It was also found that crude elastase inhibitors from six strains of A. fumigatus and two strains of A. flavus were stable to heat treatment at 100 degrees C for 10 min. In addition, human leukocyte elastases were inhibited by crude elastase inhibitors from A. fumigatus and A. flavus; however, no effect was observed on the elastase derived from porcine pancreas.
authors.affiliation: Department of Quality Control, Mathuurayakugyo Co. Ltd, 24-21 Enjo-chou, Syowa-ku, Nagoya 466-0054, Japan 2Department of Microbiology, Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tenpaku-ku, Nagoya 468-8503, Japan 3Department of Pulmonary Medicine, National Higashinagoya Hospital, 5-101 Umemorizaka, Meito-ku, Nagoya 465-8620, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Okumura
authors.affiliation: Department of Quality Control, Mathuurayakugyo Co. Ltd, 24-21 Enjo-chou, Syowa-ku, Nagoya 466-0054, Japan 2Department of Microbiology, Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tenpaku-ku, Nagoya 468-8503, Japan 3Department of Pulmonary Medicine, National Higashinagoya Hospital, 5-101 Umemorizaka, Meito-ku, Nagoya 465-8620, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Ogawa
authors.affiliation: Department of Quality Control, Mathuurayakugyo Co. Ltd, 24-21 Enjo-chou, Syowa-ku, Nagoya 466-0054, Japan 2Department of Microbiology, Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tenpaku-ku, Nagoya 468-8503, Japan 3Department of Pulmonary Medicine, National Higashinagoya Hospital, 5-101 Umemorizaka, Meito-ku, Nagoya 465-8620, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Nikai
mesh_terms: D000818:Animals; D001230:Aspergillus / Q000201:enzymology* / Q000254:growth & development; D001231:Aspergillus flavus / Q000201:enzymology / Q000254:growth & development; D001232:Aspergillus fumigatus / Q000201:enzymology / Q000254:growth & development; D001234:Aspergillus niger / Q000201:enzymology / Q000254:growth & development; D003470:Culture Media; D004795:Enzyme Stability; D006801:Humans; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D011480:Protease Inhibitors / Q000378:metabolism*
chemical_list: D003470:Culture Media; D011480:Protease Inhibitors; D010196:Pancreatic Elastase