title: Role for platelet-endothelial cell adhesion molecule-1 in macrophage Fcgamma receptor function.
abstract: Platelet-endothelial cell adhesion molecule-1 (PECAM-1) (CD31), a 130-kD transmembrane glycoprotein that functions in adhesion and signaling, is thought to play a role in some forms of leukocyte transmigration. In the lung, PECAM-1 is highly expressed, yet there have been few studies examining its role in pulmonary pathology. We therefore examined the inflammatory response (measured by bronchoalveolar lavage cell counts and protein content) after several types of lung injury in wild-type and PECAM-1 knockout mice. Consistent with studies in other organs, instillation of an endothelial stimulant (interleukin-1) was PECAM-1-dependent. In contrast, we noted that three other forms of acute lung injury (acid aspiration, adenoviral instillation, and tumor necrosis factor instillation) were completely PECAM-1-independent. Interestingly, in situ immune complex deposition injury, another complex lung disease, was also PECAM-1-dependent. This surprising finding was investigated in more detail and found to be due to a defect in macrophage activation, and not to a blockade of leukocyte transmigration. Experiments in bone marrow chimeric mice as well as ex vivo data demonstrated that Fcgamma receptor-dependent phagocytosis and tumor necrosis factor release were significantly reduced in macrophages derived from PECAM-1 knockout mice. Although PECAM-1 may not be required for transmigration of leukocytes into the alveolar space in many forms of complex lung inflammation, it is important in the function of Fcgamma receptors on alveolar macrophages.
authors.affiliation: Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA. albelda@mail.med.upenn.edu
authors.forename: Steven M
authors.initials: SM
authors.lastname: Albelda
authors.forename: Kelvin C
authors.initials: KC
authors.lastname: Lau
authors.forename: Paul
authors.initials: P
authors.lastname: Chien
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Huang
authors.forename: Eugenia
authors.initials: E
authors.lastname: Arguiris
authors.forename: Alyssa
authors.initials: A
authors.lastname: Bohen
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Billet
authors.forename: Melpo
authors.initials: M
authors.lastname: Christofidou-Solomidou
authors.forename: Zena K
authors.initials: ZK
authors.lastname: Indik
authors.forename: Alan D
authors.initials: AD
authors.lastname: Schreiber
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D008264:Macrophages / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000235:genetics / Q000502:physiology*; D011014:Pneumonia / Q000503:physiopathology; D017452:Receptors, IgG / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
chemical_list: D017931:DNA Primers; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D017452:Receptors, IgG; D014409:Tumor Necrosis Factor-alpha