Heterogeneous nuclear ribonucleoprotein E2 binds to tau exon 10 and moderately activates its splicing.
Tau is a microtubule-associated protein whose transcript undergoes complex-regulated splicing in the mammalian nervous system. Exon 10 of the gene is an alternatively spliced cassette, which is adult-specific and codes for a microtubule-binding domain. Mutations that affect splicing of exon 10 have been shown to cause frontotemporal dementia with parkinsonism (FTDP). Using tau exon 10 as a bait in a yeast three-hybrid screen, we discovered that it interacts with hnRNPE2. Cotransfection assays show that hnRNPE2 isoforms moderately activate the splicing of exon 10.
Broderick
Jennifer
J
Neurobiology Division, Shriver Center at UMMS, Waltham, MA 02452, USA.
Wang
Junning
J
Andreadis
Athena
A
D000328:Adult; D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000196:embryology / Q000378:metabolism; D019556:COS Cells; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005091:Exons / Q000235:genetics*; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000235:genetics; D016875:tau Proteins / Q000235:genetics*
D004268:DNA-Binding Proteins; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D016875:tau Proteins