The molecular scanner: concept and developments.
Approaches aimed at deciphering the proteome have illustrated the need for relatively complex and highly sensitive methodologies. The major elements of proteome analysis, such as powerful protein separation and enzymatic processing, mass spectrometry and dedicated bioinformatics have been assembled in the development of the molecular scanner. This highly flexible and data-rich approach has combined the power of electrophoretic protein separation, the simultaneous digestion and transfer of proteins through an enzymatic membrane, the immediate use of the MALDI mass spectrometer to scan a collecting membrane, and the development of dedicated bioinformatics tools to perform protein identification and molecular imaging of the proteome. Clinical applications of the molecular scanner have also started to be developed for disease diagnosis in biological material.
Binz
Pierre-Alain
PA
Swiss Institute of Bioinformatics, Proteome Informatics Group, CMU, Michel Servet 1, 1211 Geneva, Switzerland. Pierre-Alain.Binz@isb-sib.ch
Müller
Markus
M
Hoogland
Christine
C
Zimmermann
Catherine
C
Pasquarello
Carla
C
Corthals
Garry
G
Sanchez
Jean-Charles
JC
Hochstrasser
Denis F
DF
Appel
Ron D
RD
D000818:Animals; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods / Q000639:trends; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D006801:Humans; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D040901:Proteomics / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D020539:Sequence Analysis, Protein / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D018511:Systems Integration; D014584:User-Computer Interface
D011506:Proteins