The chronic colitis developed by HLA-B27 transgenic rats is associated with altered in vivo mucin synthesis.
HLA-B27 transgenic rats spontaneously developing a chronic inflammation mainly involving the colon are recognized as a powerful animal model for IBD. We investigated the mucin production in 6-month-old HLA-B27 rats by measuring in vivo fractional synthesis rate (FSR) and expression of mucins. In the inflamed colon of HLA-B27 rats, the mucin FSR was stimulated by 75% compared to F-344 controls, while MUC2,3 mRNA expression was unchanged. A local depletion in mucus-containing goblet cells was observed, suggesting a rapid mucin production/release and/or a real global decrease in goblet cell number. In the noninflamed jejunum of HLA-B27 rats, the mucin FSR was reduced by 35% compared to controls, while MUC2,3 mRNA expression was unchanged. Different alterations in mucin metabolism and expression are observed between HLA-B27 rats and a model of chemically induced chronic colitis (DSS-treated rats), suggesting that mucin alterations may be dependent on the animal model and colitis underlying mechanism.
Faure
M
M
Nestlé Research Center, Nutrition and Health Department, Nestec Ltd., Vers-chez-les-Blanc, P.O. Box 44, 1000 Lausanne 26, Switzerland. magali.faure@rdls.nestle.com
Moënnoz
D
D
Mettraux
C
C
Montigon
F
F
Schiffrin
E J
EJ
Obled
C
C
Breuillé
D
D
Boza
J
J
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D030801:Animals, Genetically Modified; D002908:Chronic Disease; D003092:Colitis / Q000276:immunology* / Q000473:pathology; D003106:Colon / Q000473:pathology; D015796:HLA-B27 Antigen / Q000235:genetics* / Q000276:immunology*; D007413:Intestinal Mucosa / Q000473:pathology; D007583:Jejunum / Q000378:metabolism; D008297:Male; D055262:Mucin-2; D055263:Mucin-3; D009077:Mucins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344
D000596:Amino Acids; D015796:HLA-B27 Antigen; C524628:Muc2 protein, rat; C524651:Muc3 protein, rat; D055262:Mucin-2; D055263:Mucin-3; D009077:Mucins; D012333:RNA, Messenger