title: Prion protein (PrPc) immunocytochemistry and expression of the green fluorescent protein reporter gene under control of the bovine PrP gene promoter in the mouse brain.
abstract: Expression of the cellular prion protein (PrP(c)) by host cells is required for prion replication and neuroinvasion in transmissible spongiform encephalopathies. As a consequence, identification of the cell types expressing PrP(c) is necessary to determine the target cells involved in the cerebral propagation of prion diseases. To identify the cells expressing PrP(c) in the mouse brain, the immunocytochemical localization of PrP(c) was investigated at the cellular and ultrastructural levels in several brain regions. In addition, we analyzed the expression pattern of a green fluorescent protein reporter gene under the control of regulatory sequences of the bovine prion protein gene in the brain of transgenic mice. By using a preembedding immunogold technique, neuronal PrP(c) was observed mainly bound to the cell surface and presynaptic sites. Dictyosomes and recycling organelles in most of the major neuron types also exhibited PrP(c) antigen. In the olfactory bulb, neocortex, putamen, hippocampus, thalamus, and cerebellum, the distribution pattern of both green fluorescent protein and PrP(c) immunoreactivity suggested that the transgenic regulatory sequences of the bovine PrP gene were sufficient to promote expression of the reporter gene in neurons that express immunodetectable endogenous PrP(c). Transgenic mice expressing PrP-GFP may thus provide attractive murine models for analyzing the transcriptional activity of the Prnp gene during prion infections as well as the anatomopathological kinetics of prion diseases.
authors.affiliation: Neurotransmission et Sécrétion Neuroendocrine UPR 2356 Centre National de la Recherche Scientifique, IFR37 des Neurosciences, 67084 Strasbourg, France. byan@neurochem.u-strasb.fr
authors.forename: Yannick
authors.initials: Y
authors.lastname: Bailly
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Haeberlé
authors.forename: Françoise
authors.initials: F
authors.lastname: Blanquet-Grossard
authors.forename: Sylvette
authors.initials: S
authors.lastname: Chasserot-Golaz
authors.forename: Nancy
authors.initials: N
authors.lastname: Grant
authors.forename: Tobias
authors.initials: T
authors.lastname: Schulze
authors.forename: Guy
authors.initials: G
authors.lastname: Bombarde
authors.forename: Jacques
authors.initials: J
authors.lastname: Grassi
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Cesbron
authors.forename: Catherine
authors.initials: C
authors.lastname: Lemaire-Vieille
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D002417:Cattle; D005786:Gene Expression Regulation / Q000502:physiology*; D017930:Genes, Reporter / Q000502:physiology*; D049452:Green Fluorescent Proteins; D007150:Immunohistochemistry; D008164:Luminescent Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D018621:PrPC Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D008164:Luminescent Proteins; D018621:PrPC Proteins; D049452:Green Fluorescent Proteins