title: [Determination of protein allergenicity--BN rat model].
abstract: OBJECTIVE
  Protein allergenicity was investigated in BN rat model.
  
  
  METHODS
  OVA or BSA was administered i.p. (1 mg/ml) or orally (10 mg/ml) to male BN rats for 6 weeks. In different intervals, blood samples were obtained from the orbital plexus for PCA and histamine determination. In addition, blood pressure of rats in OVA-treated group and control group was measured.
  
  
  RESULTS
  Relatively high titres (1/32-1/128) IgE responses were provoked by administration of OVA, while BSA stimulated only low titres (1/2-1/4) IgE. Compared with the controls, the plasma histamine concentrations of OVA groups were higher. An oral challenge with OVA did not induce a clear effect on blood pressure in the majority of rats. However, two rats demonstrated a temporary drop in blood pressure.
  
  
  CONCLUSIONS
  BN rat model may be a suitable animal model to determine protein allergenicity.
authors.affiliation: Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Jia
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Yongning
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000818:Animals; D004044:Dietary Proteins / Q000009:adverse effects*; D005512:Food Hypersensitivity / Q000209:etiology; D006632:Histamine / Q000097:blood*; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D010047:Ovalbumin / Q000009:adverse effects*; D010323:Passive Cutaneous Anaphylaxis; D011897:Random Allocation; D051381:Rats; D011914:Rats, Inbred BN; D012710:Serum Albumin, Bovine / Q000009:adverse effects*
chemical_list: D004044:Dietary Proteins; D012710:Serum Albumin, Bovine; D007073:Immunoglobulin E; D006632:Histamine; D010047:Ovalbumin