Comparative study of brain CD8+ T cells induced by sporozoites and those induced by blood-stage Plasmodium berghei ANKA involved in the development of cerebral malaria.
To obtain insight into the mechanisms that contribute to the pathogenesis of Plasmodium infections, we developed an improved rodent model that mimics human malaria closely by inducing cerebral malaria (CM) through sporozoite infection. We used this model to carry out a detailed study on isolated T cells recruited from the brains of mice during the development of CM. We compared several aspects of the immune response related to the experimental model of Plasmodium berghei ANKA infection induced by sporozoites in C57BL/6 mice and those related to a blood-stage infection. Our data show that in both models, oligoclonal TCRVbeta4(+), TCRVbeta6(+), TCRVbeta8.1(+), and TCRVbeta11(+) major histocompatibility complex class I-restricted CD8 T cells were present in the brains of CM(+) mice. These CD8(+) T cells display an activated phenotype, do not undergo apoptosis, secrete gamma interferon or tumor necrosis factor alpha, and are associated with the development of the neurological syndrome.
Bagot
Sébastien
S
Unité d'Immunophysiopathologie Infectieuse, CNRS URA 1961, Université Pierre et Marie Curie, Paris, France.
Nogueira
Fatima
F
Collette
Alexis
A
do Rosario
Virgilio
V
Lemonier
François
F
Cazenave
Pierre-André
PA
Pied
Sylviane
S
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000276:immunology* / Q000469:parasitology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D018076:DNA, Complementary / Q000235:genetics; D006183:H-2 Antigens / Q000235:genetics; D008212:Lymphocyte Depletion; D016779:Malaria, Cerebral / Q000209:etiology / Q000276:immunology* / Q000469:parasitology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010641:Phenotype; D010962:Plasmodium berghei / Q000254:growth & development / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics; D016176:T-Lymphocyte Subsets / Q000276:immunology
D018076:DNA, Complementary; D006183:H-2 Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta