title: Isolation and characterisation of new Planococcus sp. strain able for aromatic hydrocarbons degradation.
abstract: New Planococcus sp. strain S5 able to grow on salicylate or benzoate as sole carbon source was isolated from activated sludge adapted to sodium salicylate degradation. S5 was determined to be a strictly aerobic, gram-positive, catalase positive, oxidase negative, non-motile, non-spore forming coccus. The strain harboured a plasmid, named pLS5. The S5 strain when grown on salicylate expressed both catechol 1,2-dioxygenase and catechol 2,3-dioxygenase activities and degraded this substrate by both the ortho and meta pathways while grown on benzoate expressed only catechol 1,2-dioxygenase activity. Curing of the plasmid from the strain showed that plasmid pLS5 was involved in salicylate degradation by the meta pathway.
authors.affiliation: Department of Biochemistry, Faculty of Biology and Environmental Protection, University of Silesia, Jagiellońska 28, 40-032 Katowice, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Labuzek
authors.forename: Katarzyna T
authors.initials: KT
authors.lastname: Hupert-Kocurek
authors.forename: Mikael
authors.initials: M
authors.lastname: Skurnik
mesh_terms: D001565:Benzoates / Q000378:metabolism; D050548:Catechol 1,2-Dioxygenase; D050557:Catechol 2,3-Dioxygenase; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D049308:Dioxygenases; D006095:Gram-Positive Cocci / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D010105:Oxygenases / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012722:Sewage / Q000382:microbiology*; D012980:Sodium Salicylate / Q000378:metabolism*
chemical_list: D001565:Benzoates; D004269:DNA, Bacterial; D012722:Sewage; D010105:Oxygenases; D049308:Dioxygenases; D050548:Catechol 1,2-Dioxygenase; D050557:Catechol 2,3-Dioxygenase; D012980:Sodium Salicylate