title: Identification of signature genes for rapid and specific characterization of Yersinia pestis.
abstract: Polymerase chain reaction (PCR) amplification of DNA-based unique markers, the signature sequences, is ideal for rapid detection and identification of pathogens. We described the discovery of twenty-eight signature genes of Yersinia pestis by DNA microarray-based comparative genome hybridization in conjunction with PCR validation. Three pairs of Y. pestis-specific primers designed from signature genes were demonstrated to have the expected specificity to this target bacterium, without cross-reaction with the closely related Y. pseudotuberculosis or a large collection of genomic DNAs from other organisms.
authors.affiliation: National Center for Biomedical Analysis, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Bejing, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhou
authors.forename: Yanping
authors.initials: Y
authors.lastname: Han
authors.forename: Erhei
authors.initials: E
authors.lastname: Dai
authors.forename: Decui
authors.initials: D
authors.lastname: Pei
authors.forename: Yajun
authors.initials: Y
authors.lastname: Song
authors.forename: Junhui
authors.initials: J
authors.lastname: Zhai
authors.forename: Zongmin
authors.initials: Z
authors.lastname: Du
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Zhaobiao
authors.initials: Z
authors.lastname: Guo
authors.forename: Ruifu
authors.initials: R
authors.lastname: Yang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D017931:DNA Primers; D016680:Genome, Bacterial; D006801:Humans; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D015010:Yersinia pestis / Q000145:classification* / Q000235:genetics; D015011:Yersinia pseudotuberculosis / Q000145:classification / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers