title: Three-dimensional reconstruction of Haversian systems in ovine compact bone.
abstract: Haversian systems or secondary osteons are an integral component of compact bone. However, as their exact shape is debatable, this study describes a technique to view their morphology in three dimensions. Bone remodeling in adult ovine long bones was labelled at intervals using a series of chelating fluorochromes. A series of longitudinal sections were cut at 25 microm intervals through blocks of the distal radius embedded in methylmethacrylate using a sledge macrotome. The chelating agents were used as markers of bone formation in the study of bone growth and osteon morphology. The two-dimensional image of each section was examined using an epifluorescence microscope. Images were transferred to a PC via a CCD low light colour video camera. Surface reference points were noted on each of the sections and, using computer software, a three-dimensional image of a refilling labelled osteon was reconstructed and its dimensions measured. Haversian systems may have a gentle spiral course along the longitudinal axis of the bone. They intertwine with adjacent osteons and give multiple branches along their course producing a complex pattern of organization. The mean labelled length and diameter of the osteons was 1.4 + 1 mm and 145 + 0.42 microm [Mean + S.D], respectively.
authors.affiliation: Department of Anatomy, Royal College of Surgeons in Ireland, Dublin.
authors.forename: S
authors.initials: S
authors.lastname: Mohsin
authors.forename: D
authors.initials: D
authors.lastname: Taylor
authors.forename: T C
authors.initials: TC
authors.lastname: Lee
mesh_terms: D000818:Animals; D016723:Bone Remodeling; D001842:Bone and Bones / Q000033:anatomy & histology*; D006253:Haversian System / Q000033:anatomy & histology*; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence; D018715:Microscopy, Video; D012756:Sheep