Comparison of the binding properties of the mushroom Marasmius oreades lectin and Griffonia simplicifolia I-B isolectin to alphagalactosyl carbohydrate antigens in the surface phase.
The binding of two alpha-galactophilic lectins, Marasmius oreades agglutinin (MOA), and Griffonia simplicifolia I isolectin B(4) (GS I-B(4)) to neoglycoproteins and natural glycoproteins were compared in a surface phase assay. Neoglycoproteins carrying various alpha-galactosylated glycans and laminin from basement membrane of mouse sarcoma that contains the xenogenic Galalpha1-3Gal1-4GlcNAc epitope were immobilized in microtiter plate wells and lectin binding determined with an enzyme-linked assay. After 24 h of incubation, MOA had higher affinity for the xenogenic pentasaccharide (Galalpha1-3Gal1-4GlcNAcbeta1-3Galbeta1-4Glc) than for the Galalpha-monosaccharide. The binding properties of MOA and GS I-B(4) to the xenogenic disaccharide (Galalpha1-3Galbeta1) were comparable while the binding of MOA to the xenogenic pentasaccharide was much stronger than the binding of GS I-B(4) to the same epitope. Non-xenogenic disaccharide-coupled neoglycoproteins having galactose end groups linked alpha1-2 or alpha1-4 to Gal or linked alpha1-3 to GalNAc bound very weakly to MOA, whereas GS I-B(4) recognized all of these disaccharides with similarly high affinity. MOA also showed high affinity for laminin. The results indicate that the Marasmius oreades lectin has nearly the same affinities as does GS I-B(4) for the simple xenogenic carbohydrate antigens, but MOA has greater affinity for the pentasaccharide and is far more specific in its binding preferences than the Griffonia lectin.
Kirkeby
Svend
S
Department of Oral Medicine, Dental School, The Panum Institute, University of Copenhagen, Nørre Allé 20, 2200 N Copenhagen, Denmark. sk@odont.ku.dk
Winter
Harry C
HC
Goldstein
Irwin J
IJ
D000363:Agaricales; D001665:Binding Sites; D004187:Disaccharides / Q000276:immunology; D005697:Galactosides / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology; D031295:Griffonia; D007700:Kinetics; D008553:Melibiose / Q000494:pharmacology; D037121:Plant Lectins / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology
D004187:Disaccharides; D005697:Galactosides; D006023:Glycoproteins; C045475:Griffonia simplicifolia lectins; D037121:Plant Lectins; D011994:Recombinant Proteins; D008553:Melibiose