[Study on the subcloning, expression and immunoprotection with Schistosoma japonicum CAI gene].
OBJECTIVE
To subclone and express the new gene of Schistosoma japonicum (Sj) CAI and evaluate the immunoprotective effect of the recombinant molecule.


METHODS
The cDNA of SjCAI gene was subcloned into expression vector pGEX-5X-3 to form recombinants which were then used to transform to E. coli strain ER 2566. Expression was induced by IPTG. The mice were vaccinated with the expressed protein and the immunoprotective effect was tested.


RESULTS
Fusion protein of SjGST-CAI was highly expressed in E. coli as inclusion bodies. The worm reduction rate and the liver egg reduction rate in vaccination group of SjGST-CAI were 29.87% and 63.71%, respectively.


CONCLUSIONS
SjCAI gene can be highly expressed in E. coli after subcloning into pGEX-5X-3 vector and the expressed fusion protein can induce immunoprotective effect against Sj in mice.
Luo
Xiu-ju
XJ
Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078.
Yuan
Shi-shan
SS
Yi
Xin-yuan
XY
Zeng
Xian-fang
XF
Zhang
Shun-ke
SK
Tang
Lian-fei
LF
Cai
Chun
C
Zhang
Jie
J
McReynolds
Larry
L
D000818:Animals; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D015870:Gene Expression; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology; D012549:Schistosoma japonicum / Q000235:genetics* / Q000276:immunology
D015801:Helminth Proteins; D011993:Recombinant Fusion Proteins