Tuber borchii fruit body: 2-dimensional profile and protein identification.
The formation of the fruit body represents the final phase of the ectomycorrhizal fungus T. borchii life cycle. Very little is known concerning the molecular and biochemical processes involved in the fructification phase. 2-DE maps of unripe and ripe ascocarps revealed different protein expression levels and the comparison of the electropherograms led to the identification of specific proteins for each developmental phase. Associating micropreparative 2-DE to microchemical approaches, such as N-terminal sequencing and 2-D gel-electrophoresis mass-spectrometry, proteins playing pivotal roles in truffle physiology were identified.
Pierleoni
Raffaella
R
Istituto di Chimica Biologica Giorgio Fornaini, Università degli Studi di Urbino Carlo Bo, Via A. Saffi, 2, I-61029 Urbino (PU), Italy.
Buffalini
Michele
M
Vallorani
Luciana
L
Guidi
Chiara
C
Zeppa
Sabrina
S
Sacconi
Cinzia
C
Pucci
Piero
P
Amoresano
Angela
A
Casbarra
Annarita
A
Stocchi
Vilberto
V
D000595:Amino Acid Sequence; D001203:Ascomycota / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D020539:Sequence Analysis, Protein / Q000379:methods; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D005656:Fungal Proteins