[Hepatitis c virus genotype research by ABC programs of 5'-NCR restriction endonuclease digestion].
OBJECTIVE
In order to fully understand hepatitis c virus (HCV) genotype 3b, 1a, 2b and 6a infection in China, We built HCV 5'-noncoding region (5'-NCR) of different genotypes and subtypes.


METHODS
The classification HCV into variable genotypes (subtypes) was carried on by programs A, B and C A. Using a combination of three restriction endonuclease BHH' (BsrB I, Hae II, Hinf I) digestions at the same time. The distinct genotypes were classified into 5 groups: genotype 1 (1a, 1b), 6a, 2 (2a, 2b), genotype 3 (3a, 3b), genotype4 (4a). B. With regard to genotype 1, we could distinguish subtype 1a from 1b using BstU I digestion. C. Using restriction endonuclease Hae III, genotype 2a, 2b, 3b, 4a, 6a are differentiated respectively.


RESULTS
(1) HCV genotype 1a, 1b, 2a, 2b, 3a, 3b, 4a, 6a are fully discriminated by comparison with the genotypes regular samples. (2) Of the 93 patients, HCV genotype distribution in China was 66.67% for 1b, 18.28% for 2a, 3.23% for 1b/2b, 3b, 2b respectively. 2.15% for 2a/2b, 1b/2a respectively. 1.08% for 1a.


CONCLUSIONS
This research indicated that adoption of HCV 5'-NCR A B C restriction endonuclease digestions techniques, might be sensitive and efficient to detect HCV and discriminate HCV genotype (subtypes) 1a to 6a.
Qiu
Guo-hua
GH
Hepatology Institute of People's Hospital, Peking University, Beijing 100044, China.
Du
Shao-cai
SC
Sun
Nan-xiong
NX
You
Peng
P
Fan
Xiao-feng
XF
Zhang
Yong-xiang
YX
Wei
Lai
L
D020121:5' Untranslated Regions / Q000737:chemistry*; D004262:DNA Restriction Enzymes; D005838:Genotype; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics; D012367:RNA, Viral / Q000032:analysis
D020121:5' Untranslated Regions; D012367:RNA, Viral; D004262:DNA Restriction Enzymes