title: [The first report of an epidemic of human metapneumovirus infection in Japan: clinical and epidemiological study].
abstract: Human metapneumovirus (hMPV) was newly discovered as a pathogen in 2001 and is thought to be associated with respiratory disease. To elucidate the prevalence and clinical significance of hMPV among children, we investigated the positive cases of hMPV-RNA by reverse transcription-polymerase chain reaction (RT-PCR) in their nasopharyngeal specimens collected from January to August 2003 in Hiroshima Prefecture, Japan. Our prospective study revealed 77 hMPV-positive cases among 377 children with acute respiratory diseases. Clinical diagnoses of 77 hMPV-positive cases were as follows; bronchitis (33.8%), pneumonia (24.7%), acute respiratory illness (19.5%), asthmatic bronchitis (11.7%) and bronchiolitis (5.2%). The most common symptoms were cough (97.4%), high fever (94.8%) and rhinorrhea (76.6%). Most of the hMPV-positive cases were identified in the spring (between March and May), indicating the presence of an epidemic of hMPV infection in Hiroshima Prefecture. Phylogenetic analysis of the amplified F gene of hMPV isolates revealed that hMPV strains were divided into two genotypes and that their simultaneous circulation occurred within the same epidemic area of Hiroshima Prefecture.
authors.affiliation: Division of Microbiology II, Hiroshima Prefectural Institute of Health and Environment.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Takao
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Shimozono
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kashiwa
authors.forename: Keita
authors.initials: K
authors.lastname: Matsubara
authors.forename: Takashi
authors.initials: T
authors.lastname: Sakano
authors.forename: Masanori
authors.initials: M
authors.lastname: Ikeda
authors.forename: Naoko
authors.initials: N
authors.lastname: Okamoto
authors.forename: Hiromu
authors.initials: H
authors.lastname: Yoshida
authors.forename: Yukie
authors.initials: Y
authors.lastname: Shimazu
authors.forename: Shinji
authors.initials: S
authors.lastname: Fukuda
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D004196:Disease Outbreaks; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007564:Japan / Q000453:epidemiology; D008297:Male; D029121:Metapneumovirus; D018184:Paramyxoviridae Infections / Q000453:epidemiology*; D015995:Prevalence; D011446:Prospective Studies