Formalin-induced spinal glutamate release in freely moving rats: comparison of two spinal microdialysis approaches.
Two different spinal microdialysis approaches using either a linear tissue probe (LM-3) or a loop probe were explored on freely-moving rats to investigate the basal and formalin-evoked release of glutamate (Glu) in the spinal dorsal horn or in the cerebrospinal fluid (CSF). Adult male Wistar rats were implanted either with a LM-3 probe transversely through the spinal dorsal horn or with a loop probe in the CSF. After 24 hours recovery, microdialysis was initiated with perfusion of modified Ringer's solution at a flow rate of 5 microliters/min and the basal Glu concentrations were sampled for 1 hour. The effects of altering the microdialysis flow rate and perfusion solution on basal Glu release were next investigated. Following the injection of 50 microliters of formalin 5% into the hind paw, 10-min samples were collected for 90 min. The baseline levels of Glu were 0.82 +/- 0.09 microM with LM-3 probes and 5.96 +/- 0.22 microM with the loop probes. Decreasing the flow rate from 5 to 2 microliters/min increased extracellular Glu concentrations by 222.7 +/- 7.3%, whereas perfusion with artificial CSF reduced baseline Glu by 61.5 +/- 9.5% with LM-3 probes. Injection of formalin induced a short-lasting but significant increase of Glu with a similar profile and time course when using either of the microdialysis approaches. In conclusion, microdialysis in the dorsal horn or in the CSF are both effective techniques to assess the alterations in Glu release following peripheral nociceptive input. The loop probe technique in CSF is more reproducible for routine investigation of drug effects, whereas the microdialysis of the dorsal horn provides a useful tool to precisely locate where the release of the neurotransmitters occurs.
Shi
L
L
Department of Anesthesiology, AZ-VUB, Department of Physiology, Faculty of Medicine, Brussels.
Smolders
I
I
Sarre
S
S
Michotte
Y
Y
Zizi
M
M
Camu
F
F
D000704:Analysis of Variance; D000818:Animals; D005557:Formaldehyde / Q000008:administration & dosage / Q000494:pharmacology*; D018698:Glutamic Acid / Q000134:cerebrospinal fluid / Q000378:metabolism*; D007552:Isotonic Solutions / Q000008:administration & dosage; D008297:Male; D017551:Microdialysis / Q000295:instrumentation / Q000379:methods; D018377:Neurotransmitter Agents / Q000134:cerebrospinal fluid / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D012212:Rheology; D000077331:Ringer's Solution; D012636:Secretory Rate / Q000187:drug effects; D013116:Spinal Cord / Q000378:metabolism*; D013997:Time Factors
D007552:Isotonic Solutions; D018377:Neurotransmitter Agents; D005557:Formaldehyde; D018698:Glutamic Acid; D000077331:Ringer's Solution