Restricted Mobility of Cell Surface Proteins in the Polar Regions of Escherichia coli.
The polar regions of the Escherichia coli murein sacculus are metabolically inert and stable in time. Because the sacculus and the outer membrane are tightly associated, we investigated whether polar inert murein could restrict the mobility of other cell envelope elements. Cells were covalently labeled with a fluorescent reagent, chased in dye-free medium, and observed by microscopy. Fluorescent material was more efficiently retained at the cell poles than at any other location. The boundary between high and low fluorescence intensity areas was rather sharp. Labeled material consisted mostly of cell envelope proteins, among them the free and murein-bound forms of Braun's lipoprotein. Our results indicate that the mobility of at least some cell envelope proteins is restrained at regions in correspondence with underlying areas of inert murein.
de Pedro
Miguel A
MA
Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Campus de Cantoblanco, 28049 Madrid, Spain. madepedro@cbm.uam.es
Grünfelder
Christoph G
CG
Schwarz
Heinz
H
D000560:Amdinocillin / Q000494:pharmacology; D029968:Escherichia coli Proteins / Q000737:chemistry*; D005456:Fluorescent Dyes; D008565:Membrane Proteins / Q000737:chemistry*; D010457:Peptidoglycan / Q000737:chemistry*
D029968:Escherichia coli Proteins; D005456:Fluorescent Dyes; D008565:Membrane Proteins; D010457:Peptidoglycan; D000560:Amdinocillin