Study of the interactions between tetracycline analogues and lysozyme.
The interactions between tetracycline analogues (oxytetracycline, doxycycline, methacycline) and lysozyme (LYSO) were studied by using UV-visible absorption and spectrofluorimetric method. Tetracycline analogues can make LYSO's fluorescence quenching. The quenching mechanism is static quenching. Absorption spectrum and quenching constant all support this conclusion. The binding constants of tetracycline analogues with LYSO were obtained at various temperatures. According to the Förster nonradiative energy transfer theory, we can get the distances of donators and acceptors. We also determined the main acting force between them is electrostatic gravitation according to the thermodynamic parameter.
Jiang
Chong-qiu
CQ
Department of Chemistry, Shandong Normal University, Jinan 250014, China. jiangchongqiu@sdnu.edu.cn
Wang
Ting
T
D009113:Muramidase / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013754:Tetracyclines / Q000737:chemistry*
D013754:Tetracyclines; D009113:Muramidase