title: Factorial design, physicochemical characterisation and activity of ciprofloxacin-PLGA nanoparticles.
abstract: Poly(lactide-co-glycolide) nanoparticles incorporating ciprofloxacin HCl were prepared by means of a W/O/W emulsification solvent evaporation method. The stabiliser selected was poly(vinylalcohol). A 2(4) full factorial design based on four independent variables was used to plan the experiments and the variable parameters were the number of homogenisation cycles, addition of boric acid to the inner water phase containing the drug, ciprofloxacin concentration in the inner water phase and oil:outer water phase ratio. The effects of these parameters on the particle size, zeta potential, drug loading efficiency and drug release were investigated. Also the effect of gamma irradiation on the particle size and drug release was evaluated and DSC and XRD analyses of the compounds and the nanoparticles were performed. The activity on two series of microorganisms, Pseudomonas aeruginosa and Staphylococcus aureus, was examined.
authors.affiliation: Laboratory of Pharmaceutical Technology and Biopharmacy, Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp (Wilrijk), Belgium.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Dillen
authors.forename: Jo
authors.initials: J
authors.lastname: Vandervoort
authors.forename: Guy
authors.initials: G
authors.lastname: Van den Mooter
authors.forename: Loes
authors.initials: L
authors.lastname: Verheyden
authors.forename: Annick
authors.initials: A
authors.lastname: Ludwig
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002939:Ciprofloxacin / Q000737:chemistry* / Q000494:pharmacology; D003460:Crystallization; D004339:Drug Compounding; D005163:Factor Analysis, Statistical; D005720:Gamma Rays; D019344:Lactic Acid / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D036103:Nanotechnology; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012996:Solutions; D013211:Staphylococcus aureus / Q000187:drug effects; D013242:Sterilization; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D011108:Polymers; D012996:Solutions; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D002939:Ciprofloxacin