[Cloning and sequence analysis of the genome of a strain of duck hepatitis B virus].
OBJECTIVE
To clone and analyze the genome of a duck hepatitis B virus (DHBV) strain isolated from the ducks found in the local area.


METHODS
The complete genome of DHBV was amplified by PCR prior to cloning into T vector and sequencing, with also homology and phylogenetic analyses.


RESULTS
Genome comparison of an isolated DHBV strain and 16 DHBV strains in GenBank revealed a homology of 89.4% to 99.3% at the nucleotide level, and the amino acid identity of the 3 open reading frames showed that the P region harbored more obvious variations than the other regions.


CONCLUSIONS
The isolated DHBV strain might belong to a subtype of the virus found in the western countries.
Liang
Wei-fang
WF
Department of Infectious Diseases, Nangfang Hospital, First Military Medical University, Guangzhou 510515, China.
Liu
Zhi-hua
ZH
He
Hai-tang
HT
Luo
Kang-xian
KX
D000818:Animals; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000737:chemistry*; D004372:Ducks; D015611:Hepatitis B Virus, Duck / Q000145:classification / Q000235:genetics*; D016366:Open Reading Frames; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D004279:DNA, Viral