Serum pro-oxidant-antioxidant balance and low-density lipoprotein oxidation in healthy subjects with different cholesterol levels.
In this study, we investigated serum pro-oxidantantioxidant balance in 210 healthy subjects divided into groups with low and high atherogenic risk according to the levels of serum total cholesterol (TC) and low-density lipoprotein-cholesterol (LDL-C) and high-density lipoprotein- cholesterol (HDL-C). Diene conjugate (DC), malondialdehyde (MDA), polyunsaturated fatty acid (PUFA), vitamin E, and vitamin C levels and antioxidant activity (AOA) were determined in the serum. Endogenous DC and copper-induced MDA levels were also measured in the LDL fraction isolated by precipitation with buffered heparin from plasma in 80 healthy subjects with different serum LDL-C levels. Subjects with a high atherogenic risk had significantly higher plasma DC, MDA, and PUFA levels, but lower vitamin E/TC values and AOA than subjects with low atherogenic risk. Endogenous DC and copper-induced MDA levels in the LDL fraction were increased in subjects with serum LDL-C levels higher than 4.14 mM compared with those with normal LDL-C levels. In conclusion, this study clearly indicates that a disturbance in serum pro-oxidant-antioxidant balance and an increase in LDL oxidation are concomitant with higher TC and LDL-C and lower HDL-C levels in the serum.
Balkan
J
J
Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, 34093 Capa-Istanbul, Turkey.
Doğru-Abbasoğlu
S
S
Aykaç-Toker
G
G
Uysal
M
M
D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000032:analysis* / Q000378:metabolism; D002784:Cholesterol / Q000097:blood*; D003627:Data Interpretation, Statistical; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000097:blood* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D016877:Oxidants / Q000097:blood* / Q000378:metabolism; D010084:Oxidation-Reduction
D000975:Antioxidants; D008077:Lipoproteins, LDL; D016877:Oxidants; D002784:Cholesterol