Detection of bovine respiratory syncytial virus in experimentally infected balb/c mice.
The present study used an RT-nested-PCR and an immunohistochemistry assay to detect bovine respiratory syncytial virus in tissues from experimentally infected balb/c mice. As a first step, Chicken Embryo Related (CER) cell monolayers infected with the BRSV-25-BR strain isolated in Brazil were used for antigen production. Then, the infected lung and tracheal tissues of female balb/c mice were collected on 3, 5, 7 and 10 days post-infection and submitted to both techniques. Primers specific to F and G genes that amplify fragments of 481 bp and 371 bp, respectively, were used. The BRSV detection was not successful in all of the animals tested. The genomic fragment of the G gene from the organs of some infected mice on all analyzed post-infection days was amplified. However, in the RT-nested-PCR corresponding to the F gene, it was not possible to observe any amplified fragment. This was probably due to the higher sensitivity of the developed technique to amplify the fragment corresponding to the G gene compared to the F gene. Moreover, only three of the lungs collected five days post-infection were positive by immunohistochemistry. To the author's knowledge, this is the first study reporting bovine respiratory syncytial virus detection in balb/c mice after experimental inoculation.
Almeida
Renata Servan
RS
Laboratório de Virologia Animal, Departamento de Microbiologia e Imunologia, Instituto de Biologia, Universidade Estadual de Campinas-UNICAMP, 13081-970 Campinas, SP, Brazil.
Domingues
Helena Gallicchio
HG
Coswig
Lia Treptow
LT
D'Arce
Regina Celia Freitas
RC
de Carvalho
Rodrigo Franco
RF
Arns
Clarice Weis
CW
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D002642:Chick Embryo / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D051379:Mice; D008807:Mice, Inbred BALB C; D018357:Respiratory Syncytial Virus Infections / Q000662:veterinary* / Q000821:virology; D018112:Respiratory Syncytial Virus, Bovine / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012680:Sensitivity and Specificity; D013047:Specific Pathogen-Free Organisms
D004279:DNA, Viral