Expression of LacZ gene in canine muscle by intramuscular inoculation of a plasmid DNA.
DNA immunization induces systemic humoral and cellular immune responses to the antigen encoded by cDNA in a plasmid DNA. In the present study, a plasmid DNA encoding cDNA of beta-galactosidase (beta-gal), pCAGGS-lacZ, was inoculated intramuscularly to a healthy dog in order to evaluate location and duration of the gene expression. On day 7, the plasmid DNA was found by PCR in the muscle where the plasmid was injected. Furthermore, beta-gal expression was detected in the same muscle sample by beta-gal staining. However, the plasmid DNA was not detected in any samples collected on days 14, 21 and 28. The present results suggest that duration of the gene expression of beta-gal by the plasmid DNA is limited in the muscle in dogs and an efficacy for a gene expression should be evaluated depending on the gene inserted in the plasmid DNA for immunotherapy.
Maeda
Sadatoshi
S
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
Ohmori
Keitaro
K
Kurata
Keigo
K
Sakaguchi
Masahiro
M
Masuda
Kenichi
K
Ohno
Koichi
K
Tsujimoto
Hajime
H
D000818:Animals; D004247:DNA / Q000378:metabolism*; D017931:DNA Primers; D004285:Dogs / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D007273:Injections, Intramuscular; D018482:Muscle, Skeletal / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D013997:Time Factors; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism*
D017931:DNA Primers; D004247:DNA; D001616:beta-Galactosidase