[Assessment of hematopoiesis regeneration in patients with solid tumors after radiotherapy].
The myelotoxicity is one of the most severe adverse events of radiotherapy. Increase of CD34+ cells level in peripheral blood as result of raised output of granulocyte colony stimulating factor (G-CSF) can be result of hematopoiesis regeneration after radiotherapy. The aim of this study was to determine the hematopoiesis regeneration using analysis of CD34+ cells level in peripheral blood and serum concentration of G-CSF in patients treated with radiotherapy according to irradiated body region and irradiation field size. Two groups of irradiated patients were examined. Group I consisted of 11 patients (mean age 56) with gynecological malignancies (teletherapy dose 40-50 Gy for pelvic area and brachytherapy with Cs). Group II consisted of 10 patients (mean age 58) with head and neck malignancies (teletherapy only 50-70 Gy). Every patient was evaluated 3 times: before radiotherapy, in the day of ending and 14 days after therapy. 3 ml of blood for CD34 and serum for G-CSF estimation were collected. Blood cells were stained with monoclonal antibody specific for CD34 antigen and analysed by flow cytometry. G-CSF level was estimated by ELISA. After radiotherapy in both groups statistically significant leukopenia (p < 0.001) was observed. There was no difference between two groups in levels of CD34+ cells before and in the last day of therapy but there was significant increase of CD34+ cells in group I compared with group II 14 days after treatment (p < 0.01). Decrease of CD34+ cells during radiotherapy and after its ending in all patients was observed but only in group II was statistically significant. Positive correlation between amount of leukocytes and CD34+ cells percentage was stated. There were no statistically significant differences in serum G-CSF concentration within particular groups and between group I and II. Our results indicate that evaluation of CD34+ cells level in peripheral blood is useful in prediction of hematopoiesis regeneration after radiotherapy. G-CSF serum concentration is not prognostic factor in these groups of patients.
Mazur
Grzegorz
G
Klinika Hematologii, Nowotworów Krwi i Transplantacji Szpiku AM we Wrocławiu.
Wróbel
Tomasz
T
Włodarska-Polińska
Iwona
I
Łacko
Aleksandra
A
Bojarowska
Katarzyna
K
Jagas
Maria
M
Kuliczkowski
Kazimierz
K
D000368:Aged; D018952:Antigens, CD34 / Q000097:blood / Q000528:radiation effects; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D005833:Genital Neoplasms, Female / Q000532:radiotherapy*; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood / Q000528:radiation effects; D006258:Head and Neck Neoplasms / Q000532:radiotherapy*; D006410:Hematopoiesis / Q000528:radiation effects*; D006412:Hematopoietic Stem Cells / Q000528:radiation effects; D017619:Hemibody Irradiation / Q000009:adverse effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011867:Radioisotope Teletherapy / Q000009:adverse effects*; D012038:Regeneration; D018709:Statistics, Nonparametric
D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor