Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation.
The Saccharomyces cerevisiae gene RRP1 encodes an essential, evolutionarily conserved protein necessary for biogenesis of 60S ribosomal subunits. Processing of 27S pre-ribosomal RNA to mature 25S rRNA is blocked and 60S subunits are deficient in the temperature-sensitive rrp1-1 mutant. We have used recent advances in proteomic analysis to examine in more detail the function of Rrp1p in ribosome biogenesis. We show that Rrp1p is a nucleolar protein associated with several distinct 66S pre-ribosomal particles. These pre-ribosomes contain ribosomal proteins plus at least 28 nonribosomal proteins necessary for production of 60S ribosomal subunits. Inactivation of Rrp1p inhibits processing of 27SA(3) to 27SB(S) pre-rRNA and of 27SB pre-rRNA to 7S plus 25.5S pre-rRNA. Thus, in the rrp1-1 mutant, 66S pre-ribosomal particles accumulate that contain 27SA(3) and 27SB(L) pre-ribosomal RNAs.
Horsey
Edward W
EW
Department of Biological Sciences, Carnegie Mellon University, 616 Mellon Institute, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA.
Jakovljevic
Jelena
J
Miles
Tiffany D
TD
Harnpicharnchai
Piyanun
P
Woolford
John L
JL
D000595:Amino Acid Sequence; D002466:Cell Nucleolus / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D012322:RNA Precursors / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D012269:Ribosomal Proteins; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
D009687:Nuclear Proteins; D012322:RNA Precursors; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; C485288:Rrp1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C065039:RNA, ribosomal, 25S