title: Deciphering enzymes. Genetic selection as a probe of structure and mechanism.
abstract: The efficient engineering of enzymes with novel activities remains an ongoing challenge. Towards this end, genetic selection techniques provide a method for finding rare solutions to catalytic problems that requires only a limited foreknowledge of structure-function relationships. We have used genetic selections to extensively probe the structure and mechanism of chorismate mutases. The insights gained from these investigations will aid future enzyme design efforts.
authors.affiliation: Laboratorium für Organische Chemie, Swiss Federal Institute of Technology, ETH-Hönggerberg, Zürich, Switzerland.
authors.forename: Kenneth J
authors.initials: KJ
authors.lastname: Woycechowsky
authors.forename: Donald
authors.initials: D
authors.lastname: Hilvert
mesh_terms: D001665:Binding Sites; D002384:Catalysis; D002826:Chorismate Mutase / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D015202:Protein Engineering; D017510:Protein Folding; D017433:Protein Structure, Secondary; D012641:Selection, Genetic; D013329:Structure-Activity Relationship
chemical_list: D002826:Chorismate Mutase