title: Quantitative assessment of in vitro interactions implicates TATA-binding protein as a target of the VP16C transcriptional activation region.
abstract: Models of mechanisms of transcriptional activation in eukaryotes frequently invoke direct interactions of transcriptional activation domains with target proteins including general transcription factors or coactivators such as chromatin modifying complexes. The potent transcriptional activation domain (AD) of the VP16 protein of herpes simplex virus has previously been shown to interact with several general transcription factors including the TATA-binding protein (TBP), TBP-associated factor 9 (TAF9), TFIIA, and TFIIB. In surface plasmon resonance assays, a module of the VP16 AD designated VP16C (residues 452-490) bound to TBP with an affinity notably stronger than to TAF9, TFIIA or TFIIB. Moreover, the interaction of VP16C with TBP correlated well with transcriptional activity for a panel of VP16C substitution variants. These results support models in which the interactions of ADs with TBP play an important role in transcriptional activation.
authors.affiliation: Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824-1319, USA.
authors.forename: Yuri A
authors.initials: YA
authors.lastname: Nedialkov
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Triezenberg
mesh_terms: D000818:Animals; D017405:Herpes Simplex Virus Protein Vmw65 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D018139:Simplexvirus / Q000378:metabolism*; D035181:TATA-Box Binding Protein / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D015533:Transcriptional Activation
chemical_list: D017405:Herpes Simplex Virus Protein Vmw65; D011993:Recombinant Fusion Proteins; D035181:TATA-Box Binding Protein