Preparation of highly active alpha-chymotrypsin for catalysis in organic media.
A simple one step process for the preparation of free alpha-chymotrypsin, using an organic solvent to precipitate the enzyme from a buffered solution, followed by washing with organic solvents, is described. This preparation gave 132 times greater esterification activity than lyophilized powder.
Roy
Ipsita
I
Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India.
Gupta
Munishwar N
MN
D002384:Catalysis; D002918:Chymotrypsin / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D009930:Organic Chemicals / Q000737:chemistry*
D009930:Organic Chemicals; D002918:Chymotrypsin; C117608:alpha-chymotrypsin