title: Comparative serological evaluation of 10 Blastomyces dermatitidis yeast phase lysate antigens from different sources.
abstract: Yeast phase lysate antigens from 10 isolates of Blastomyces dermatitidis (dog, ERC-2 and T-58; T-27, polar bear; woodpile, ER-3; bat lung, 48938; human, B5929, B5895, B5896, B5931, and CAPP) from different geographical regions, in addition to a Histoplasma capsulatum (G217B) lysate preparation were compared with respect to their reactivity against serum specimens from dogs, rabbits and humans positive for blastomycosis using an indirect enzyme-linked immunosorbent assay. In addition, the lysate antigens were also assayed against histoplasmosis-positive human serum samples to study their cross-reactivity. Variable results were obtained with T-58 and T-27 exhibiting the greatest reactivity. We also noticed that the lysate did not react consistently to serum samples across species with lesser reactivity evidenced when testing dog sera. Finally, T-58 gave the highest cross-reactivity with histoplasmosis-positive sera. The study may prove valuable in the development of antigen candidates for blastomycosis serodiagnosis.
authors.affiliation: College of Health Sciences, University of Sharjah, Sharjah, United Arab Emirates. abuodeh@sharjah.ac.ae
authors.forename: R O
authors.initials: RO
authors.lastname: Abuodeh
authors.forename: E M
authors.initials: EM
authors.lastname: Chester
authors.forename: G M
authors.initials: GM
authors.lastname: Scalarone
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood*; D000946:Antigens, Fungal / Q000276:immunology*; D001758:Blastomyces / Q000276:immunology*; D001759:Blastomycosis / Q000175:diagnosis / Q000276:immunology* / Q000382:microbiology / Q000662:veterinary; D003429:Cross Reactions; D004283:Dog Diseases / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007106:Immune Sera; D011817:Rabbits; D001503:Ursidae
chemical_list: D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D007106:Immune Sera