Functional relevance of the stretch-dependent slow force response in failing human myocardium.
Stretch induces immediate and delayed inotropic effects in mammalian myocardium via distinct mechanosensitive pathways, but these effects are poorly characterized in human cardiac muscle. We tested the effects of stretch on immediate and delayed force response in failing human myocardium. Experiments were performed in muscle strips from 52 failing human hearts (37 degrees C, 1 Hz, bicarbonate buffer). Muscles were stretched from 88% of optimal length to 98% of optimal length. The resulting immediate and delayed (ie, slow force response [SFR]) increases in twitch force were assessed without and after blockade of the sarcoplasmic reticulum (SR; cyclopiazonic acid and ryanodine), stretch-activated ion channels (SACs; gadolinium, streptomycin), L-type Ca2+-channels (diltiazem), angiotensin II type-1 (AT1) receptors (candesartan), endothelin (ET) receptors (PD145065 or BQ123), Na+/H+ exchange (NHE1; HOE642), or reverse-mode Na+/Ca+ exchange (NCX; KB-R7493). We also tested the effects of stretch on SR Ca2+ load (rapid cooling contractures [RCCs]) and intracellular pH (in BCECF-loaded trabeculae). Stretch induced an immediate (<10 beats), followed by a slow (5 to 10 minutes), force response. Twitch force increased to 232+/-6% of prestretch value during the immediate phase, followed by a further increase to 279+/-8% during the SFR. RCC amplitude significantly increased, but pHi did not change during SFR. Inhibition of SACs, L-type Ca2+ channels, AT1 receptors, or ET receptors did not affect the stretch-dependent immediate or SFR. In contrast, the SFR was reduced by NHE1 inhibition and almost completely abolished by reverse-mode NCX inhibition or blockade of sarcoplasmic reticulum function. The data demonstrate the existence of a functionally relevant, SR-Ca2+-dependent SFR in failing human myocardium, which partly depends on NHE1 and reverse-mode NCX activation.
von Lewinski
Dirk
D
Department of Cardiology and Pneumology, Georg-August-University, Göttingen, Germany.
Stumme
Burkhard
B
Fialka
Florian
F
Luers
Claus
C
Pieske
Burkert
B
D001696:Biomechanical Phenomena; D020746:Calcium Channels, L-Type / Q000502:physiology; D002303:Cardiac Output, Low / Q000378:metabolism / Q000503:physiopathology*; D002311:Cardiomyopathy, Dilated / Q000503:physiopathology; D065128:Endothelin Receptor Antagonists; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007473:Ion Channels / Q000502:physiology; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D009200:Myocardial Contraction; D017202:Myocardial Ischemia / Q000503:physiopathology; D044140:Receptor, Angiotensin, Type 1 / Q000502:physiology; D019831:Sodium-Calcium Exchanger / Q000502:physiology*; D017923:Sodium-Hydrogen Exchangers / Q000502:physiology*
D020746:Calcium Channels, L-Type; D065128:Endothelin Receptor Antagonists; D007473:Ion Channels; D044140:Receptor, Angiotensin, Type 1; D019831:Sodium-Calcium Exchanger; D017923:Sodium-Hydrogen Exchangers