title: Crystallization and preliminary crystallographic studies of the D59A mutant of MicA, a YycF response-regulator homologue from Streptococcus pneumoniae.
abstract: RR02 (MicA) is an essential bacterial protein that belongs to the YycF family of response regulators and consists of two domains: an N-terminal receiver domain and a C-terminal effector domain. Streptococcus pneumoniae RR02 (MicA; residues 2-234) has been crystallized using the sitting-drop vapour-diffusion technique. The crystals belong to space group P2(1), with unit-cell parameters a = 46.46, b = 32.61, c = 63.35 A, beta = 90.01 degrees. X-ray diffraction data have been collected to 1.93 A resolution.
authors.affiliation: IBLS, Division of Infection and Immunity, University of Glasgow, Glasgow G12 8QQ, Scotland.
authors.forename: Alan
authors.initials: A
authors.lastname: Riboldi-Tunnicliffe
authors.forename: Marie Claude
authors.initials: MC
authors.lastname: Trombe
authors.forename: Colin J
authors.initials: CJ
authors.lastname: Bent
authors.forename: Neil W
authors.initials: NW
authors.lastname: Isaacs
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Mitchell
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D009154:Mutation; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013296:Streptococcus pneumoniae / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D047908:Intracellular Signaling Peptides and Proteins; C495423:MicA protein, Streptococcus pneumoniae; D011994:Recombinant Proteins; C119939:YycF protein, Bacteria