Auto-accumulation method using simulated annealing enables fully automatic particle pickup completely free from a matching template or learning data.
Single-particle analysis is a 3-D structure determining method using electron microscopy (EM). In this method, a large number of projections is required to create 3-D reconstruction. In order to enable completely automatic pickup without a matching template or a training data set, we established a brand-new method in which the frames to pickup particles are randomly shifted and rotated over the electron micrograph and, using the total average image of the framed images as an index, each frame reaches a particle. In this process, shifts are selected to increase the contrast of the average. By iterated shifts and further selection of the shifts, the frames are induced to shift so as to surround particles. In this algorithm, hundreds of frames are initially distributed randomly over the electron micrograph in which multi-particle images are dispersed. Starting with these frames, one of them is selected and shifted randomly, and acceptance or non-acceptance of its new position is judged using the simulated annealing (SA) method in which the contrast score of the total average image is adopted as an index. After iteration of this process, the position of each frame converges so as to surround a particle and the framed images are picked up. This method is the first unsupervised fully automatic particle picking method which is applicable to EM of various kinds of proteins, especially to low-contrasted cryo-EM protein images.
Ogura
Toshihiko
T
Neuroscience Research Institute and Biological Information Research Center, National Institute of Advanced Industrial Science and Technology, Umezono 1-1-4, Tsukuba, Ibaraki 305-8568, Japan.
Sato
Chikara
C
D000465:Algorithms; D000818:Animals; D001052:Apoferritins / Q000737:chemistry; D001331:Automation; D020285:Cryoelectron Microscopy / Q000379:methods*; D004524:Eels; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008968:Molecular Conformation; D010316:Particle Size; D015222:Sodium Channels / Q000737:chemistry
D015222:Sodium Channels; D001052:Apoferritins