In vivo 13C-NMR and modelling study of metabolic yield response to ethanol stress in a wild-type strain of Saccharomyces cerevisiae.
In this paper the combined use of in vivo (13)C-nuclear magnetic resonance spectroscopy and mathematical modelling allowed the analysis of the response to ethanol stress in a wild-type strain of Saccharomyces cerevisiae, in terms of a reduced metabolic activity. The model developed succeeded in describing and interpreting the effects of increasing concentrations of exogenous ethanol. In particular, the ratio between the kinetic constants associated with ethanol production and glucose consumption gave the estimation of the metabolic yield of the processes in perfect agreement with experimental results.
Martini
Silvia
S
Dipartimento di Scienze e Tecnologie Chimiche e dei Biosistemi, Università di Siena, Via Aldo Moro 2, 53100 Siena, Italy.
Ricci
Maso
M
Bonechi
Claudia
C
Trabalzini
Lorenza
L
Santucci
Annalisa
A
Rossi
Claudio
C
D002247:Carbon Isotopes; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000378:metabolism / Q000494:pharmacology*; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D008954:Models, Biological; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D002247:Carbon Isotopes; D000431:Ethanol; D005947:Glucose