Expression of calretinin by fetal otocyst cells after transplantation into damaged rat utricle explants.
Severe damage by acoustic overstimulation or ototoxins induces inner ear hair cell loss, resulting in permanent hearing loss and balance disorders because hair cell regeneration scarcely occurs in the inner ear sensory organs of mammals. In this study, to evaluate the possibilities of cell transplantation therapy for damaged inner ear sensory organs, dissociated cell cultures of fetal otocyst cells (FOCs) were established from embryonic day 12.5 (E12.5) rat inner ears, and transplanted into gentamicin-treated explants of vestibular sensory epithelia. Two weeks after transplantation, immunohistochemical analysis demonstrated that some of the grafted FOCs survived within the vestibular sensory epithelia and expressed epitopes of calretinin. one of the hair cell marker proteins. These findings indicate that FOCs have the potential to migrate into damaged vestibular epithelia and differentiate into hair cell immunophenotypes. Cell transplantation therapy may be available for functional regeneration in inner ear diseases.
Kim
Tae-Soo
TS
Department of Otolaryngology--Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Kojima
Ken
K
Nishida
Akiko T
AT
Tashiro
Kei
K
Lee
Ji-Eun
JE
Fujino
Kiyohiro
K
Nakagawa
Takayuki
T
Naito
Yasushi
Y
Omori
Koichi
K
Lefebvre
Phillippe
P
Ito
Juichi
J
D000818:Animals; D064032:Calbindin 2; D018929:Cell Culture Techniques; D007758:Ear, Inner / Q000166:cytology / Q000196:embryology* / Q000473:pathology; D004848:Epithelium / Q000502:physiology; D006198:Hair Cells, Auditory / Q000166:cytology*; D007150:Immunohistochemistry; D016130:Immunophenotyping; D007381:Intermediate Filament Proteins / Q000378:metabolism; D007759:Labyrinth Diseases / Q000209:etiology / Q000628:therapy*; D008856:Microscopy, Fluorescence; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D051381:Rats; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D012444:Saccule and Utricle / Q000166:cytology / Q000473:pathology; D033581:Stem Cell Transplantation / Q000379:methods; D014945:Wound Healing / Q000502:physiology
C576512:Calb2 protein, rat; D064032:Calbindin 2; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D064030:S100 Calcium Binding Protein G