[Genotyping of HBV DNA in Shenzhen and clinical manifestations].
OBJECTIVE
To detect HBV DNA and its genotypes.


METHODS
The 6 isoforms of HBV DNA was detected out using of different probes by Polymerase Chain Reaction and Nucleic Acid hybridization.


RESULTS
Of 150 HBV DNA positive patients who lived in Shenzhen, 50 samples (33%) are type B, 36 samples (24%) are type C, 13 samples (9%) are type D, 3 samples is type F, 1 sample is type A, 48 samples (31%) are mixed type. The ALT value was significantly higher in genotype B than in genotype C. HBe positivity were higher in genotype B than genotype C. HBeAg positivity were higher in genotype C than in genotype B. There are not obvious relations between genotype and age or sex.


CONCLUSIONS
In the detected samples, the major genotype of HBV DNA is type B, several are type C, D. The type E haven't been found. There are some relations between all kinds of genotypes and the severity of hepatitis B.
Dai
Jian-yi
JY
The Department of Digestion Medicine of Shenzhen People's Hospital, Shenzhen 518020, China.
Shi
Zhi-lin
ZL
Dai
Yong
Y
Du
Hong
H
Chen
De-heng
DH
Wang
Sha-yan
SY
D000328:Adult; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005838:Genotype; D006509:Hepatitis B / Q000821:virology*; D006515:Hepatitis B virus / Q000145:classification* / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction
D004279:DNA, Viral