title: Regeneration of mastoid air cells: clinical applications.
abstract: The objective of this study was to establish a method for regenerating mastoid air cells and their functions for clinical use in incurable otitis media. For this clinical study three patients (one male, two female) were randomly selected from patients with severe cholesteatoma about to undergo staged operations. Hydroxy-apatite in three-dimensional, honeycomb-like structures (3D-HA) were used as artificial pneumatic bones. This 3D-HA is made of calcium phosphate and has a high percentage of micropores (90%). Its surface is coated with collagen. At the first stage of tympanoplasty, collagen-coated 3D-HA was put into the opened mastoid cavity and fixed by fibrin glue. Recovery of mastoid aeration and regeneration of the pneumatic air cells of the mastoid cavity were estimated on CT scan images after the first operation. Aeration was recovered in all cases. The mastoid air cells were regenerated in two cases. In the failed case, subcutaneous connective tissues and granulations invaded into the spaces of the 3D-HA. This study demonstrated that mucosa would grow on the surface of a 3D-HA implant and could provide gas exchange functions in the newly opened mastoid cavity. This tissue engineering method may be a possible treatment for intractable otitis media.
authors.affiliation: Department of Otolaryngology--Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan. kanemaru@ent.kuhp.kyoto-u.ac.jp
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Kanemaru
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Nakamura
authors.forename: Koichi
authors.initials: K
authors.lastname: Omori
authors.forename: Akhmar
authors.initials: A
authors.lastname: Magrufov
authors.forename: Masaru
authors.initials: M
authors.lastname: Yamashita
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Haruo
authors.initials: H
authors.lastname: Takahashi
authors.forename: Juichi
authors.initials: J
authors.lastname: Ito
mesh_terms: D000368:Aged; D002781:Cholesteatoma / Q000601:surgery*; D017886:Durapatite / Q000737:chemistry; D005260:Female; D006801:Humans; D008297:Male; D008416:Mastoid / Q000166:cytology / Q000502:physiology* / Q000601:surgery*; D008875:Middle Aged; D012038:Regeneration / Q000502:physiology*; D023822:Tissue Engineering; D014057:Tomography, X-Ray Computed; D016896:Treatment Outcome
chemical_list: D017886:Durapatite