title: Human S15a expression is upregulated by hepatitis B virus X protein.
abstract: The hepatitis B virus (HBV)-encoded X antigen (HBxAg) may contribute to the development of hepatocellular carcinoma (HCC) through the upregulated expression of selected cellular genes. To identify these genes, RNAs isolated from HBxAg-positive and -negative HepG2 cells were compared by PCR select cDNA subtraction. One gene overexpressed in HBxAg-positive cells by Northern and Western blotting is the ribosomal protein S15a. The S15a mRNA is 535 base pairs, encoding a protein 130 amino acids long with a molecular weight of 14.3 kDa. S15a expression was upregulated in HBV-infected livers, where it costained with HBxAg. Overexpression of S15a stimulated cell growth, colony formation in soft agar, and tumor formation in SCID mice. Hence, HBxAg upregulated the expression of S15a, the latter of which participates in the development of HCC, perhaps by altering the integrity of translation.
authors.affiliation: Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107-6749, USA.
authors.forename: Zhaorui
authors.initials: Z
authors.lastname: Lian
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Xianxing
authors.initials: X
authors.lastname: Li
authors.forename: N Lale Satiroglu
authors.initials: NL
authors.lastname: Tufan
authors.forename: Meng-Chao
authors.initials: MC
authors.lastname: Wu
authors.forename: Hong-Yang
authors.initials: HY
authors.lastname: Wang
authors.forename: Patrick
authors.initials: P
authors.lastname: Arbuthnot
authors.forename: Michael
authors.initials: M
authors.lastname: Kew
authors.forename: Mark A
authors.initials: MA
authors.lastname: Feitelson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D003001:Cloning, Molecular; D003114:Colony-Forming Units Assay; D018076:DNA, Complementary; D015972:Gene Expression Regulation, Neoplastic; D006511:Hepatitis B Antigens / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D016513:Mice, SCID; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism; D013382:Subtraction Technique; D015534:Trans-Activators / Q000235:genetics*; D015854:Up-Regulation; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D018076:DNA, Complementary; D006511:Hepatitis B Antigens; D012269:Ribosomal Proteins; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; C074488:ribosomal protein S15a