Virulence properties of rough and smooth strains of Vibrio cholerae O1.
A comparative study was carried out to see the differences in pathogenicity of rough and smooth strains. A total of 10 strains including 5 each of rough and smooth strains of Vibrio cholerae O1 were tested and found positive for toxin production by enzyme-linked immunosorbent assay (ELISA) in Richardson's and AKI media. All the smooth and rough strains, except one, showed a titre of 1: 10 and 1: 100 in Richardson's and AKI media, respectively. Both types of strains produced enterotoxin in rabbit ileal loop (RIL). The differences in multiplication abilities of smooth and rough strains in RIL were statistically significant (P <0.05). However, these differences in multiplying abilities did not influence the adherence potential or enterotoxin production as there was no significant difference (P >0.05) between these properties. This study demonstrated that the rough strains are equally pathogenic and as important as smooth strains.
Islam
Mohammad Sirajul
MS
International Centre for Diarrhoeal Disease Research, Bangladesh. sislam@icddrb.org
Ahsan
Sunjukta
S
Khan
Sirajul Islam
SI
Ahmed
Qazi Shafi
QS
Rashid
Mohammad Harunur
MH
Islam
Khan M Nasirul
KM
Sack
Richard Bradley
RB
D000818:Animals; D001422:Bacterial Adhesion; D002772:Cholera Toxin / Q000096:biosynthesis; D003470:Culture Media; D006801:Humans; D007082:Ileum / Q000382:microbiology / Q000503:physiopathology; D010641:Phenotype; D011817:Rabbits; D041241:Vibrio cholerae O1 / Q000472:pathogenicity* / Q000502:physiology*; D014774:Virulence
D003470:Culture Media; D002772:Cholera Toxin