title: Crystallization and preliminary crystallographic analysis of BbCRASP-1, a complement regulator-acquiring surface protein of Borrelia burgdorferi.
abstract: Borrelia burgdorferi is the causative agent of Lyme disease. Serum-resistant strains of the pathogen are able to reduce the host's immune response to infection by recruiting fluid-phase complement regulators from the serum. B. burgdorferi complement regulator-acquiring surface protein-1 (BbCRASP-1) binds factor H and factor-H-like protein-1 to the bacterial surface, where they actively down-regulate complement response. Crystals of native and selenomethionine-substituted BbCRASP-1 have been obtained and a native data set to 2.7 A as well as selenomethionine MAD data to 3.2 A resolution have been collected. The selenium substructure has been solved and initial phases have been refined to 3.0 A by density-modification methods. Model building and refinement are under way.
authors.affiliation: Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, England.
authors.forename: Frank S
authors.initials: FS
authors.lastname: Cordes
authors.forename: Peter
authors.initials: P
authors.lastname: Kraiczy
authors.forename: Pietro
authors.initials: P
authors.lastname: Roversi
authors.forename: Christine
authors.initials: C
authors.lastname: Skerka
authors.forename: Michael
authors.initials: M
authors.lastname: Kirschfink
authors.forename: Markus M
authors.initials: MM
authors.lastname: Simon
authors.forename: Volker
authors.initials: V
authors.lastname: Brade
authors.forename: Edward D
authors.initials: ED
authors.lastname: Lowe
authors.forename: Peter
authors.initials: P
authors.lastname: Zipfel
authors.forename: Reinhard
authors.initials: R
authors.lastname: Wallich
authors.forename: Susan M
authors.initials: SM
authors.lastname: Lea
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001665:Binding Sites; D025065:Borrelia burgdorferi / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012643:Selenium / Q000737:chemistry / Q000378:metabolism; D012645:Selenomethionine / Q000737:chemistry / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; C440651:complement regulator-acquiring surface proteins, Borrelia burgdorferi; D012645:Selenomethionine; D012643:Selenium