title: Mass spectrometric detection of protein, lipid and heme components of cytochrome c oxidase from R. sphaeroides and the stabilization of non-covalent complexes from the enzyme.
abstract: The cytochrome c oxidase enzyme from the Rhodobacter sphaeroides bacteria exists as a complex of four peptide subunits, two hemes, and a variety of lipids and metal ions held together by non-covalent forces. While the native enzyme functions as an associated unit, this complex usually dissociates during MALDI- TOF analysis. Through the use of matrix additives such as sucrose, the complete complex and partial complexes can be stabilized in the MALDI-TOF experiment. The dissociation of the complex allows for the detection of the components of the enzyme. The direct detection of associated lipids from an aqueous solution of the intact enzyme may eliminate the need for enzyme disruption and lipid extraction. The partial dissociation of multisubunit enzymes in such experiments may allow for the determination of subunit-subunit and subunit-lipid interactions
authors.affiliation: Department of Chemistry, Michigan State University, East Lansing, MI 48824-1322, USA.
authors.forename: Anne M
authors.initials: AM
authors.lastname: Distler
authors.forename: John
authors.initials: J
authors.lastname: Allison
authors.forename: Carrie
authors.initials: C
authors.lastname: Hiser
authors.forename: Ling
authors.initials: L
authors.lastname: Qin
authors.forename: Yasmin
authors.initials: Y
authors.lastname: Hilmi
authors.forename: Shelagh
authors.initials: S
authors.lastname: Ferguson-Miller
mesh_terms: D000595:Amino Acid Sequence; D003576:Electron Transport Complex IV / Q000737:chemistry*; D006418:Heme / Q000032:analysis* / Q000737:chemistry; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D008969:Molecular Sequence Data; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D012242:Rhodobacter sphaeroides / Q000737:chemistry / Q000201:enzymology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D008055:Lipids; D011506:Proteins; D006418:Heme; D003576:Electron Transport Complex IV