title: Inability of simian virus 40 to establish productive infection of lymphoblastic cell lines.
abstract: Lymphoblastic cell lines were infected with simian virus 40 (SV40) and then monitored for evidence of a productive infection. No evidence of early gene expression was found 2 days following infection, as determined by assaying viral mRNAs and early antigens. Furthermore, only small amounts of virus could be detected by plaque assay 2 days after infection, and levels slowly declined until they were undetectable after a few weeks in culture. Thus, human lymphocytes are not readily infectible with SV40 and do not provide a simple model for studying interactions of SV40 with a human cell type.
authors.affiliation: Department of Microbiology-Immunology, Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, Illinois 60611, USA.
authors.forename: Sophie
authors.initials: S
authors.lastname: Shaikh
authors.forename: Christine
authors.initials: C
authors.lastname: Skoczylas
authors.forename: Richard
authors.initials: R
authors.lastname: Longnecker
authors.forename: Kathleen
authors.initials: K
authors.lastname: Rundell
mesh_terms: D002460:Cell Line; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D006801:Humans; D008214:Lymphocytes / Q000821:virology*; D013539:Simian virus 40 / Q000472:pathogenicity*; D010948:Viral Plaque Assay