title: Cross-clade HIV-1 neutralization by an antibody fragment from a lupus phage display library.
abstract: A single-chain fragment containing antibody V domains (scFv) isolated from a lupus antibody library displayed the ability to bind gp120 and the conserved gp120 determinant composed of residues 421-436. The scFv neutralized R5 and X4-dependent HIV-1 strains from clades B, C, and D. The lupus repertoire may be useful as a source of neutralizing antibodies to HIV.
authors.affiliation: Chemical Immunology Research Center, Department of Pathology, University of Texas, Houston Medical School, USA.
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Karle
authors.forename: Stephanie
authors.initials: S
authors.lastname: Planque
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Nishiyama
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Taguchi
authors.forename: Yong-Xin
authors.initials: YX
authors.lastname: Zhou
authors.forename: Maria
authors.initials: M
authors.lastname: Salas
authors.forename: Douglas
authors.initials: D
authors.lastname: Lake
authors.forename: Perumal
authors.initials: P
authors.lastname: Thiagarajan
authors.forename: Frank
authors.initials: F
authors.lastname: Arnett
authors.forename: Carl V
authors.initials: CV
authors.lastname: Hanson
authors.forename: Sudhir
authors.initials: S
authors.lastname: Paul
mesh_terms: D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000378:metabolism*; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007128:Immunoglobulin Fragments / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology
chemical_list: D000939:Epitopes; D015699:HIV Envelope Protein gp120; D007128:Immunoglobulin Fragments