title: Sperm count in earthworms (Lumbricus terrestris) as a biomarker for environmental toxicology: effects of cadmium and chlordane.
abstract: Earthworms, Lumbricus terrestris, exposed in artificial soil to sublethal concentrations of technical chlordane (6.25, 12.5, and 25 ppm) and cadmium nitrate (100, 200, and 300 ppm) exhibited significant reduction in spermatozoa from testes and seminal vesicles. The onset time of reduction varied with exposure concentration, but absolute depression in sperm count was independent of exposure concentration or exposure duration after reduction was first manifested, demonstrating a threshold effect. Earthworm sperm counts show potential as a rapid-measurement endpoint biomarker for measuring sublethal effects of chemical pollutants on reproduction.
authors.affiliation: Departamento de Ciencias Biologicas, Facultad de Ciencias de la Salud, Universidad de Antofagasta, Antofagasta, Chile.
authors.forename: M A
authors.initials: MA
authors.lastname: Cikutovic
authors.forename: L C
authors.initials: LC
authors.lastname: Fitzpatrick
authors.forename: B J
authors.initials: BJ
authors.lastname: Venables
authors.forename: A J
authors.initials: AJ
authors.lastname: Goven