title: In vitro RNA editing systems from higher plant chloroplasts.
abstract: RNA editing in higher plant chloroplasts involves C-to-U conversion at ~30 specific sites. In vitro systems supporting accurate editing have been developed from tobacco and pea chloroplasts. mRNA substrates labeled with 32P at C residues to be edited provide sensitive detection of editing activity in vitro. The present systems allow the rapid identification of cis-elements using mutated mRNA substrates and trans-acting factors by ultraviolet crosslinking.
authors.affiliation: School of Biomedical Science, Tokyo Medical and Dental University, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Hirose
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Miyamoto
authors.forename: Junichi
authors.initials: J
authors.lastname: Obokata
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sugiura
mesh_terms: D001483:Base Sequence; D002458:Cell Fractionation / Q000379:methods; D002736:Chloroplasts / Q000235:genetics* / Q000648:ultrastructure; D003596:Cytosine; D018532:Pisum sativum / Q000235:genetics; D010944:Plants / Q000235:genetics*; D017393:RNA Editing / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics*; D012680:Sensitivity and Specificity; D014026:Nicotiana / Q000235:genetics; D014498:Uracil
chemical_list: D012333:RNA, Messenger; D018749:RNA, Plant; D014498:Uracil; D003596:Cytosine