Chlorpromazine interactions to sera albumins. A study by the quenching of fluorescence.
Binding of chlorpromazine (CPZ) and hemin (Hmn) to human (HSA) and bovine (BSA) serum albumin was studied by fluorescence quenching technique. Intrinsic fluorescences of BSA and HSA were measured by selectively exciting their tryptophan residues. Gradual quenching was observed by titration of both proteins with CPZ and Hmn. CPZ is a widely used anti-psychosis drug that causes severe side effects and strongly interacts with biomembranes, both in its lipidic and proteic regions. CPZ also interacts with blood components, influences bioavailability, and affects the function of several biomolecules. Albumin plays an important role in the transport and storage of hormones, ions, fatty acids and others substances, including CPZ, affecting the regulation of their plasmatic concentration. Hmn is an important ferric residue of hemoglobin that binds within the hydrophobic region of albumin with great specificity. Hmn added to HSA and BSA solutions at a molar ratio of 1:1 quenched about half of their fluorescence. Stern-Volmer plots obtained from experiments carried out at 25 and 35 degrees C showed the quenching of fluorescence of HSA and BSA by CPZ to be a collisional phenomenon. Hmn quenches fluorescence by a static process, which specifically indicates the formation of a complex. Our results suggest the prime binding site for CPZ and Hmn on both HSA and BSA to be near tryptophan residues.
Silva
Dilson
D
Department of Physiology, Rio de Janeiro State University, Rio de Janeiro, Brazil. dilsons@uerj.br
Cortez
Célia M
CM
Louro
Sônia R W
SR
D000418:Albumins / Q000737:chemistry*; D000818:Animals; D014150:Antipsychotic Agents / Q000737:chemistry* / Q000494:pharmacology; D002417:Cattle; D002746:Chlorpromazine / Q000737:chemistry* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006427:Hemin / Q000737:chemistry; D006801:Humans; D008956:Models, Chemical; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature
D000418:Albumins; D014150:Antipsychotic Agents; D012709:Serum Albumin; D006427:Hemin; D002746:Chlorpromazine