title: Comparison between different sheathless electrospray emitter configurations regarding the performance of nanoscale liquid chromatography-time-of-flight mass spectrometry analysis.
abstract: Four different sheathless electrospray ionization (ESI) configurations were investigated for a nano liquid chromatography (LC) system. The studied configurations were: a column with an integrated emitter, with the ESI potential applied before or after the column, and a column with separate emitter, with the ESI voltage applied at a union before the emitter or at the emitter tip. The results indicates that the efficiency of the LC system is rather independent of the configuration when using 95 microm i.d. columns, acetic mobile phase and standard peptides as a sample. Introduction of post column dead volume seems not to be a critical issue at least with flow rates down to 600 nl/min.
authors.affiliation: Department of Analytical Chemistry, Institute of Chemistry, Uppsala University, PO Box 599, SE-75124 Uppsala, Sweden.
authors.forename: Ardeshir
authors.initials: A
authors.lastname: Amirkhani
authors.forename: Magnus
authors.initials: M
authors.lastname: Wetterhall
authors.forename: Stefan
authors.initials: S
authors.lastname: Nilsson
authors.forename: Rolf
authors.initials: R
authors.lastname: Danielsson
authors.forename: Jonas
authors.initials: J
authors.lastname: Bergquist
mesh_terms: D036103:Nanotechnology; D010449:Peptide Mapping; D012710:Serum Albumin, Bovine / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014357:Trypsin / Q000737:chemistry
chemical_list: D012710:Serum Albumin, Bovine; D014357:Trypsin