DNA-templated Ag nanocluster formation.
The high affinity of Ag+ for DNA bases has enabled creation of short oligonucleotide-encapsulated Ag nanoclusters without formation of large nanoparticles. Time-dependent formation of cluster sizes ranging from Ag1 to Ag4/oligonucleotide were observed with strong, characteristic electronic transitions between 400 and 600 nm. The slow nanocluster formation kinetics enables observation of specific aqueous nanocluster absorptions that evolve over a period of 12 h. Induced circular dichroism bands confirm that the nanoclusters are associated with the chiral ss-DNA template. Fluorescence, absorption, mass, and NMR spectra all indicate that multiple species are present, but that their creation is both nucleotide- and time-dependent.
Petty
Jeffrey T
JT
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332-0400 USA. jeff.petty@furman.edu
Zheng
Jie
J
Hud
Nicholas V
NV
Dickson
Robert M
RM
D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D021241:Spectrometry, Mass, Electrospray Ionization; D013698:Templates, Genetic
D009841:Oligonucleotides; D012834:Silver; D004247:DNA