Primary murine cells as a model for HIV-1 infection.
Immortalized and transduced cell lines are traditionally used in model of the HIV-1 life cycle. Primary cells may better represent the tissue of origin and events in vivo. We utilized an HIV-1/murine leukemia A4070 pseudotype virus and human Cyclin T1 to replicate HIV-1 in primary murine cells, and demonstrate that primary murine cells support HIV-1 infection better than immortalized cells.
Lund
Lars H
LH
Microbiology and Tumor Biology Center and Department of Virology, Swedish Institute for Infectious Disease Control, Karolinska Institute, 17182 Solna, Sweden.
Ljungberg
Karl
K
Wahren
Britta
B
Hinkula
Jorma
J
D000818:Animals; D002460:Cell Line; D004195:Disease Models, Animal; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D014779:Virus Replication