title: [Effect of testosterone on expression of androgen receptor in human monocytic cell line THP-1].
abstract: OBJECTIVE
  To investigate the effect of testosterone on the expression of androgen receptor (AR) in human monocytic cell line THP-1.
  
  
  METHODS
  Cultured THP-1 cells were stimulated by testosterone with different concentrations and the AR protein was examined by Western blotting.
  
  
  RESULTS
  AR expression was observed in unstimulated THP-1 cells and the quantity of AR expression was reduced by testosterone treatment in a dose-dependent manner. A selective AR blocker completely abolished this inhibitory effect of testosterone on AR expresison.
  
  
  CONCLUSIONS
  Testosterone simulation time- and dose dependently reduces AR expression in THP-1 cells.
authors.affiliation: Department of Cardiology, Nanfang Hostipal, First Military Medical University, Guangzhou 510515, China. liucqing@fimmu.com
authors.forename: Chang-qing
authors.initials: CQ
authors.lastname: Liu
authors.forename: Sai-zhu
authors.initials: SZ
authors.lastname: Wu
authors.forename: Zi-dong
authors.initials: ZD
authors.lastname: Wang
authors.forename: Wen-yang
authors.initials: WY
authors.lastname: Lai
authors.forename: Fei
authors.initials: F
authors.lastname: Sun
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D006801:Humans; D009000:Monocytes / Q000737:chemistry / Q000187:drug effects*; D011944:Receptors, Androgen / Q000032:analysis*; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D011944:Receptors, Androgen; D013739:Testosterone