title: Porcine choroid plexus epithelial cells induce Streptococcus suis bacteriostasis in vitro.
abstract: The involvement of the choroid plexus in host defense during bacterial meningitis is unclear. Aiming to elucidate possible antibacterial mechanisms, we stimulated primary porcine choroid plexus epithelial cells (pCPEC) with proinflammatory cytokines and challenged them with various Streptococcus suis strains. In the supernatant of gamma interferon (IFN-gamma)-stimulated pCPEC, streptococcal growth was markedly suppressed. Costimulation with tumor necrosis factor alpha enhanced this bacteriostatic effect, while supplementation of L-tryptophan completely eliminated it. We also demonstrate that an activation of indoleamine 2,3-dioxygenase in the pCPEC seems to be responsible for the IFN-gamma-induced bacteriostasis. This supports the hypothesis of an active role of the choroid plexus in host defense against bacterial meningitis.
authors.affiliation: Klinik für Allgemeine Pädiatrie. Institut für Medizinische Mikrobiologie, Universitätsklinikum Düsseldorf, Düsseldorf, Germany.
authors.forename: Rüdiger A
authors.initials: RA
authors.lastname: Adam
authors.forename: Tobias
authors.initials: T
authors.lastname: Tenenbaum
authors.forename: Peter
authors.initials: P
authors.lastname: Valentin-Weigand
authors.forename: Maurice
authors.initials: M
authors.lastname: Laryea
authors.forename: Bernd
authors.initials: B
authors.lastname: Schwahn
authors.forename: Susanne
authors.initials: S
authors.lastname: Angelow
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Galla
authors.forename: Walter
authors.initials: W
authors.lastname: Däubener
authors.forename: Horst
authors.initials: H
authors.lastname: Schroten
mesh_terms: D000818:Animals; D002831:Choroid Plexus / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D004789:Enzyme Activation; D004847:Epithelial Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000494:pharmacology; D016920:Meningitis, Bacterial / Q000276:immunology; D011994:Recombinant Proteins / Q000494:pharmacology; D013290:Streptococcal Infections / Q000276:immunology; D017011:Streptococcus suis / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D034421:Sus scrofa; D014364:Tryptophan / Q000378:metabolism; D014366:Tryptophan Oxygenase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D014364:Tryptophan; D014366:Tryptophan Oxygenase