Fast fibres in a large animal: fibre types, contractile properties and myosin expression in pig skeletal muscles.
Little is known about the influence of Myosin Heavy Chain (MHC) isoforms on the contractile properties of single muscle fibres in large animals. We have studied MHC isoform composition and contractile properties of single muscle fibres from the pig. Masseter, diaphragm, longissimus, semitendinosus, rectractor bulbi and rectus lateralis were sampled in female pigs (aged 6 months, mass 160 kg). RT-PCR, histochemistry, immunohistochemistry and gel electrophoresis were combined to identify and separate four MHC isoforms: MHC-slow and three fast MHC (2A, 2X, 2B). Maximum shortening velocity (V(o)) and isometric tension (P(o)) were measured in single muscle fibres with known MHC isoform composition. Six groups of fibres (pure: slow, 2A, 2X and 2B, and hybrid: 2A-2X and 2X-2B) with large differences in V(o) and P(o) were identified. Slow fibres had mean V(o)=0.17+/-0.01 length s(-1) and P(o)=25.1+/-3.3 mN mm(-2). For fast fibres 2A, 2X and 2B, mean V(o) values were 1.86+/-0.18, 2.55+/-0.19 and 4.06+/-0.33 length s(-1) and mean P(o) values 74.93+/-8.36, 66.85+/-7.58 and 32.96+/-7.47 mN mm(-2), respectively. An in vitro motility assay confirmed that V(o) strictly reflected the functional properties of the myosin isoforms. We conclude that pig muscles express high proportions of fast MHC isoforms, including MHC-2B, and that V(o) values are higher than expected on the basis of the scaling relationship between contractile parameters and body size.
Toniolo
Luana
L
Department of Anatomy and Physiology, University of Padova, 35131 Padova, Italy.
Patruno
Marco
M
Maccatrozzo
Lisa
L
Pellegrino
Maria A
MA
Canepari
Monica
M
Rossi
Rosetta
R
D'Antona
Giuseppe
G
Bottinelli
Roberto
R
Reggiani
Carlo
C
Mascarello
Francesco
F
D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D006651:Histocytochemistry; D007150:Immunohistochemistry; D009119:Muscle Contraction / Q000502:physiology*; D018485:Muscle Fibers, Skeletal / Q000502:physiology*; D018482:Muscle, Skeletal / Q000502:physiology*; D018995:Myosin Heavy Chains / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D034421:Sus scrofa / Q000378:metabolism* / Q000502:physiology
D017931:DNA Primers; D018076:DNA, Complementary; D018995:Myosin Heavy Chains