High-energy collision-induced dissociation of oxosteroids derivatised to Girard hydrazones.
Neutral oxosteroids have been derivatised with Girard T and P hydrazine reagents to give the corresponding Girard hydrazone quaternary ammonium salts. Both Girard T (GT) and Girard P (GP) hydrazones of oxosteroids give very intense [M](+) ion signals in electrospray (ES) mass spectra and fragment within the ES interface and collision cell to give characteristic fragment ions. GT and GP derivatives give informative high-energy collision-induced dissociation spectra, from which the structure of the precursor oxosteroid can be determined. Both charge-remote and charge-mediated mechanisms are responsible for the formation of the fragment ions at high collision-energy.
Griffiths
William J
WJ
Department of Pharmaceutical & Biological Chemistry, The School of Pharmacy, University of London, 29-39 Brunswick Square, London WC1N 1AX, UK.
Alvelius
Gunvor
G
Liu
Suya
S
Sjövall
Jan
J
D006835:Hydrazones / Q000032:analysis / Q000737:chemistry*; D007664:Ketosteroids / Q000737:chemistry*; D007700:Kinetics; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization; D013739:Testosterone / Q000031:analogs & derivatives / Q000737:chemistry
D006835:Hydrazones; D007664:Ketosteroids; D013739:Testosterone