title: Effect of cadmium on germination, amylases and rate of respiration of germinating pea seeds.
abstract: Growth of embryonic axis of germinating pea seeds (Pisum sativum cv. Bonneville) was significantly inhibited by as low as 0.25 mM cadmium and the elongation of the radicle was affected more severely than that of the plumule. Total amylolytic activity, as well as activities of alpha- and beta-amylases, diminished progressively with increasing concentrations of the metal in the media. The deleterious effect on alpha-amylase persisted throughout, whereas beta-amylase activity recovered with time. The rate of respiration of seeds, measured by oxygen uptake, was markedly impeded by cadmium and the phase of rapid and linear development of respiratory activity (after 3 days of imbibition) was almost completely, suppressed in the presence of higher concentrations (1 mM) of the metal. The observed impact of cadmium on starch mobilization and on respiratory activity are discussed in relation to its adverse effects on seed germination.
authors.affiliation: Department of Chemistry and Biochemistry, CCS Haryana Agricultural University, Hisar, India.
authors.forename: L K
authors.initials: LK
authors.lastname: Chugh
authors.forename: S K
authors.initials: SK
authors.lastname: Sawhney