Identification of a novel 29-linked polyubiquitin binding protein, Ufd3, using polyubiquitin chain analogues.
Lysine 48-linked polyubiquitin chains are the best understood form of polyubiquitin and are necessary for the function of the ubiquitin-proteasome system. However, other forms of polyubiquitin (e.g., K29- and K63-linked chains) are also present in vivo. Less is known about the functional roles of these linkages or the proteins specifically interacting with these forms of polyubiquitin. Use of native polyubiquitin chains to identify binding proteins is complicated by the difficulties of synthesis and stability. Here, we report the synthesis of a nonhydrolyzable analogue of 29-linked polyubiquitin chains on an affinity support and its use in identifying proteins that bind 29-linked polyubiquitin chains. The 29-linked Ub4 resin was stable and tightly bound recombinant human Isopeptidase T (USP5), a deubiquitinating enzyme known to bind the 29-linked polyubiquitin chains. Two high affinity interactors of the 29-linked polyubiquitin analogues were identified from Saccharomyces cerevisiae lysates. They were identified as Ubp14, the yeast ortholog of Isopeptidase T, and Ufd3, a member of the ubiquitin-fusion degradation pathway with unknown function. Purified recombinant Ufd3 bound to the resin as well, confirming that Ufd3 is a novel binding partner of polyubiquitin. These results demonstrate the efficacy of using polyubiquitin analogue affinity supports to identify novel binding partners of specifically linked polyubiquitin chains. Identification of these proteins will lead to a greater understanding of the physiological relevance of different polyubiquitin linkages.
Russell
Nathaniel S
NS
Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
Wilkinson
Keith D
KD
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D019759:Carbon-Nitrogen Lyases; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002474:Cell-Free System / Q000378:metabolism; D019281:Dimerization; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008239:Lysine / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D017354:Point Mutation; D025821:Polyubiquitin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000302:isolation & purification / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D029867:Xenopus Proteins / Q000378:metabolism; D014982:Xenopus laevis
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; C099944:DOA1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D029867:Xenopus Proteins; D025821:Polyubiquitin; D010450:Endopeptidases; C108364:UBP14 protein, S cerevisiae; C091939:ubiquitin isopeptidase; D019759:Carbon-Nitrogen Lyases; C034300:isopeptidase; D008239:Lysine