title: Antiprotozoal effect of crude extracts and flavonoids isolated from Chromolaena hirsuta (asteraceae).
abstract: Phytochemical study of Chromolaena hirsuta (Hook. & Arn.) R. King & H. Robinson crude extract (collected in Furnas, MG-Brasil) produced fifteen flavonoids, two triterpenes and five steroids. IR, UV,(1)H and (13)C NMR spectroscopy and GC analysis were used for the identification of these compounds. The dichloromethanic and ethanolic crude extracts (flowers and leaves) and six flavonoids isolated from this specie have been assayed for antiprotozoal activity against tripomastigote forms of Trypanosoma cruzi and promastigote forms of Leishmania amazonensis. The crude extracts significantly reduced the viability of T. cruzi and Leishmania amazonensis, and the six flavonoids showed considerable antiproliferative effect of development of two parasites evaluated. This is the fi rst report of antiprotozoal activity of extracts of C. hirsuta.
authors.affiliation: Departamento de Química, Faculdade de Filoso fi a Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, (SP), Brasil.
authors.forename: S H
authors.initials: SH
authors.lastname: Taleb-Contini
authors.forename: M J
authors.initials: MJ
authors.lastname: Salvador
authors.forename: J M F
authors.initials: JM
authors.lastname: Balanco
authors.forename: S
authors.initials: S
authors.lastname: Albuquerque
authors.forename: D C R
authors.initials: DC
authors.lastname: de Oliveira
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D036604:Chromolaena; D005419:Flavonoids / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D035264:Flowers; D007891:Leishmania / Q000187:drug effects*; D021261:Parasitic Sensitivity Tests; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves; D014349:Trypanosoma cruzi / Q000187:drug effects*
chemical_list: D000981:Antiprotozoal Agents; D005419:Flavonoids; D010936:Plant Extracts