Supplementation with cysteamine during maturation and embryo culture on embryo development of prepubertal goat oocytes selected by the brilliant cresyl blue test.
Our previous studies have shown that the addition of 100 mircroM cysteamine to the in vitro maturation (IVM) medium increased the embryo development of prepubertal goat oocytes. The aim of the present study was to evaluate the effect of adding different concentrations of cysteamine to the IVM medium and to the in vitro embryo culture medium (IVC) on the embryo development of prepubertal goat oocytes selected by the brilliant cresyl blue (BCB) test. Oocytes were exposed to BCB and classified as: oocytes with a blue cytoplasm or grown oocytes (BCB+) or oocytes without blue cytoplasm or growing oocytes (BCB-). In Experiment 1, oocytes were matured in a conventional IVM medium supplemented with 100 microM, 200 microM or 400 microM cysteamine. In Experiment 2, oocytes were matured with 400 microM cysteamine and following in vitro fertilization (IVF) were cultured in SOF medium supplemented with 50 microM and 100 microM cysteamine. In Experiment 1, BCB+ oocytes matured with 100 microM and 200 microM cysteamine showed higher normal fertilization and embryo development rates than BCB- oocytes. Oocytes matured with 400 microM cysteamine did not present these differences between BCB+ and BCB- oocytes. In Experiment 2, the addition of 50 microM and 100 microM cysteamine to culture medium did not affect the proportion of total embryos obtained from BCB+ oocytes (35.89% and 38.29%, respectively) but was significantly different in BCB- oocytes (34.23% and 29.04%, respectively, P < 0.05). In conclusion, the addition of 400 microM cysteamine to the IVM improved normal fertilization and embryo development of BCB- oocytes at the same rates as those obtained from BCB+ oocytes. The proportions of morulae plus blastocyst development were not affected by the treatments.
Urdaneta
Aixa
A
Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
Jiménez-Macedo
Ana-Raquel
AR
Izquierdo
Dolors
D
Paramio
Maria-Teresa
MT
D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D018929:Cell Culture Techniques; D003470:Culture Media / Q000737:chemistry; D003543:Cysteamine / Q000494:pharmacology*; D005314:Embryonic and Fetal Development / Q000187:drug effects / Q000502:physiology*; D005307:Fertilization in Vitro; D006041:Goats; D008297:Male; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D010078:Oxazines / Q000737:chemistry
D003470:Culture Media; D010078:Oxazines; C006797:Brilliant Cresyl Blue; D003543:Cysteamine