B-cell repertoire reconstitution after hematopoietic stem cell transplantation in children evaluated by immunoglobulin heavy chain third complementarity determining region fingerprinting.
Immunodeficiency after hematopoietic stem cell transplantation leads to a high risk of opportunistic infections. We evaluated B-lymphocyte reconstitution in 36 children by heavy chain third complementarity determining region (CDR3)-fingerprinting and immunophenotypic analysis. The time necessary to return to the normal immunoglobulin heavy chain-CDR3 polyclonal situation was basically related to the type of transplant and this process did not recapitulate fetal ontogenesis.
Di Martino
Daniela
D
Dallorso
Sandro
S
Terranova
Paola
P
Scarso
Lucia
L
Morreale
Giuseppe
G
Valetto
Angelo
A
D000293:Adolescent; D001402:B-Lymphocytes / Q000502:physiology*; D002648:Child; D002675:Child, Preschool; D022801:Complementarity Determining Regions / Q000235:genetics*; D016172:DNA Fingerprinting; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007107:Immune System / Q000502:physiology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007223:Infant; D007700:Kinetics; D038041:Lymphopoiesis; D008297:Male; D012038:Regeneration
D022801:Complementarity Determining Regions; D007143:Immunoglobulin Heavy Chains