Development of 99mTc labelled Lipiodol: biodistribution following injection into the hepatic artery of the healthy pig.
BACKGROUND
We develop a method for the radiolabelling of Lipiodol with Tc, using a lipophilic complex, [99mTc-(S2CPh)(S3Ph)2], dissolved in Lipiodol (99mTc-SSS Lipiodol).


RESULTS
The labelling yield is high (96 +/- 0.8%), and the radiochemical purity satisfactory (92 +/- 2.6%). This labelling is reproducible and stable for up to 24 h in vitro. Studies carried out after injection into the hepatic artery of the healthy pig show that the biodistribution of 99mTc-SSS Lipiodol is comparable with that observed for 188Re Lipiodol.


METHODS
The 99mTc-SSS lipiodol was obtained after dissolving a chelating agent, previously labelled with 99mTc, in cold lipiodol. The radiochemical purity (RCP) of the labelling was checked immediately and at 24 h. The 99mTc-SSS lipiodol was injected into the hepatic artery of four healthy pigs for an ex-vivo biodistribution study. An autoradiographic study was performed in two cases.


CONCLUSIONS
Apart from the specific interest of a Lipiodol-bearing technetiated agent for carrying out dosimetric studies, the labelling of Lipiodol with 99mTc is a preliminary step towards the use of radiolabelling with the 188Re analogue.
Garin
Etienne
E
Service de Médecine Nucléaire, Centre Eugène Marquis, Rennes, France. etienne.garin@univ-rennes1.fr
Noiret
Nicolas
N
Malbert
Charles-Henri
CH
Lepareur
Nicolas
N
Roucoux
Alain
A
Dazord
Léontine
L
Caulet-Maugendre
Sylvie
S
Turlin
Bruno
B
Moisan
Annick
A
Lecloirec
Joseph
J
Herry
Jean-Yves
JY
Boucher
Eveline
E
Raoul
Jean-Lue
JL
Bourguet
Patrick
P
D000818:Animals; D005240:Feasibility Studies; D006499:Hepatic Artery / Q000187:drug effects / Q000378:metabolism*; D008658:Inactivation, Metabolic; D007269:Injections, Intra-Arterial; D007459:Iodized Oil / Q000737:chemistry* / Q000493:pharmacokinetics*; D007553:Isotope Labeling / Q000379:methods*; D009928:Organ Specificity; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000493:pharmacokinetics; D013552:Swine; D013667:Technetium / Q000737:chemistry* / Q000493:pharmacokinetics*; D014018:Tissue Distribution
D019275:Radiopharmaceuticals; D013667:Technetium; D007459:Iodized Oil