Inhibitory effects of extracellular matrix modulator(s) on lipopolysaccharide (LPS)- and acidosis-damaged glia containing cerebellar granule cell cultures.
The inhibitory effects of a novel chondroitin sulfate compound on lipopolysaccharide (LPS)- and acidosis-induced neuronal dysfunctions were examined. Cell viabilities in cultured neurons and/or astrocyte-rich cerebellar granule cells were measured by the calcein-AM method. Ten and 20 microg, as a final dosage, of LPS damaged less than 20% cells during a-2 h incubation. More than 5000 ng/ml of chondroitin sulphate-dipalmitoylphosphatidylethanolamine (CS-PE), but not chondroitin sulfate (CS) treatment, significantly inhibited such damage. Twenty microg of LPS damaged more than 40% cells during 24 h incubation, and these cell damages were significantly inhibited by less than 1000 ng/ml of CS-PE. Moreover, treatments with between 5 and 500 ng/ml CS-PE, but not CS, significantly reduced the number of acidosis-damaged cells in a dose-dependent manner. The current results indicate that modulator(s) of ECM and its derivative containing covalently linked dipalmitoylphosphatidylethanolamine show neuroprotective effects under conditions of brain inflammation.
Fukui
Hidekimi
H
Department of Anesthesiology, Tokyo Medical University, Tokyo, Japan.
Fukui
Yoko
Y
Noguchi
Masashi
M
Kameyama
Yoshiyuki
Y
Isshiki
Atsushi
A
Watanabe
Yasuo
Y
D000138:Acidosis / Q000473:pathology*; D000818:Animals; D002531:Cerebellum / Q000187:drug effects* / Q000473:pathology; D003594:Cytoplasmic Granules / Q000187:drug effects*; D005109:Extracellular Matrix / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009457:Neuroglia / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
D008070:Lipopolysaccharides