[Effect of apoptosis in human mammary cancer (MCF-7) cells induced by cis9, t11-conjugated linoleic acid].
OBJECTIVE
To determine the effect of cell apoptosis induced by c9, t11-conjugated linoleic acid was investigated in human mammary cancer cells (MCF-7), which were treated with various c9, t11-CLA concentrations (25, 50, 100 and 200 mumol/L), with a negative control.


METHODS
In this study, the methods of the curve of cell growth, cell apoptosis detecting in fluorescent and electronic microscope and flow cytometry, and expression of p53 protein were employed.


RESULTS
The cell growth of MCF-7 cells was inhibited by c9, t11-CLA. Eight day after treatment with various concentrations of c9, t11-CLA, as mentioned above, the inhibition rates were -6.0%, 45.2%, 99.0% and 99.4%, respectively. Apoptotic cells of morphology from MCF-7 cells treated by different concentrations of c9, t11-CLA were observed by fluorescent and electronic microscope and the frequency of apoptosis in MCF-7 cells by flow cytometry showed increasingly with concentrations of c9, t11-CLA increased. While the expression of p53 protein in MCF-7 cells was decreased with the doses of c9, t11-CLA increased.


CONCLUSIONS
c9, t11-CLA can induce the effect of apoptosis in MCF-7 cells and its may be one of mechanisms for the inhibitory effect of c9, t11-CLA in human cancer cells.
Liu
Jiaren
J
Public Health College, Harbin Medical University, Harbin 150001, China.
Chen
Bingqing
B
Yang
Yanmei
Y
Wang
Xuanlin
X
D016587:Antimutagenic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D005260:Female; D006801:Humans; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis / Q000235:genetics
D016587:Antimutagenic Agents; D044243:Linoleic Acids, Conjugated; D016159:Tumor Suppressor Protein p53