New phenotypic, functional and electrophysiological characteristics of KG-1 cells.
Myeloid dendritic cells (DC) are representatives of a rare and phenotypically diverse population of professional antigen presenting cells possessing high functional heterogeneity and flexibility. Here we studied the phenotypic, functional and electrophysiological characteristics of KG-1 cells, an erythroleukemia model cell line, which shares morphological and physiological similarities with immature and mature myeloid DC. We compared the expression of internalizing receptors and other cell surface molecules, antigen uptake and migration of unstimulated and activated KG-1 cells with the characteristics of immature and mature DC. Unstimulated KG-1 cells were less potent in capturing extracellular materials than immature DC. In contrast to monocyte-derived DC KG-1 cells stimulated by PMA and ionomycin ceased to migrate along the MIP-3beta chemokine gradient despite their high expression of CCR7 chemokine receptor and MDR, a transporter implicated in DC migration. Moreover, we determined the ion channel repertoire of KG-1 cells before and after treatment with PMA and ionomycin by using the patch-clamp technique. We found that both unstimulated and activated KG-1 cells expressed time- and voltage-independent, ChTx sensitive intracellular Ca(2+)-gated potassium conductance suggesting the presence of K(Ca) channels in their membranes. Based on our results we propose that KG-1 cells resemble myeloid DC but also possess unique phenotypic, functional and electrophysiological characteristics.
Hajas
György
G
Faculty of Medicine, Research Centre for Molecular Medicine, Medical and Health Science Center, Institute of Immunology, University of Debrecen, Debrecen H-4012, Hungary.
Zsiros
Emese
E
László
Tünde
T
Hajdú
Péter
P
Somodi
Sándor
S
Réthi
Bence
B
Gogolák
Péter
P
Ludányi
Katalin
K
Panyi
György
G
Rajnavölgyi
Eva
E
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002465:Cell Movement / Q000276:immunology / Q000502:physiology; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D003911:Dextrans / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D007546:Isoquinolines / Q000378:metabolism; D004915:Leukemia, Erythroblastic, Acute / Q000276:immunology* / Q000378:metabolism; D018408:Patch-Clamp Techniques; D014407:Tumor Cells, Cultured
D003911:Dextrans; D007546:Isoquinolines; C015219:fluorescein isothiocyanate dextran; C017475:lucifer yellow; D016650:Fluorescein-5-isothiocyanate; D002118:Calcium