title: [The role of peroxisome proliferators-activated receptor gamma (PPARgamma) during activation of hepatic stellate cells].
abstract: OBJECTIVE
  To observe the role of PPARgamma during the activation process of hepatic stellate cells (HSC).
  
  
  METHODS
  By morphology and RT-PCR, we study the changes of expression of PPARgamma in culture-activated HSC or in vivo activated HSC induced by dimethylnitrosamine (DMN).
  
  
  RESULTS
  In vitro, the expression level of PPARgamma in freshly isolated HSC (0.72+/-0.01) significantly reduced to 0.48+/-0.03 on the third day of culture (t = 19.8372, P<0.01), and reduced 70% on the seventh culture-day and could not be detected after the second passage. In vivo, HSC freshly isolated from normal control rats expressed PPARgamma (0.76+/-0.01). During the development of rat liver fibrosis induced by DMN, the expression level significantly reduced to 0.46+/-0.02 after the third injection of DMN (t = 29.5318, P<0.01), and reduced 66% on the end of first week and could not be detected on the end of second and third week.
  
  
  CONCLUSIONS
  The expression of PPARgamma might play an important role on the maintenance of resting-form of HSC, and the reduction of expression of PPARgamma might be an early event during the activation process of HSC.
authors.affiliation: Tongji Hospital of Tongji University, Shanghai 200065, China.
authors.forename: Wen-zhuo
authors.initials: WZ
authors.lastname: Yang
authors.forename: Rui-lin
authors.initials: RL
authors.lastname: Liu
authors.forename: Min-de
authors.initials: MD
authors.lastname: Zeng
authors.forename: Lun-gen
authors.initials: LG
authors.lastname: Lu
authors.forename: Xi-mei
authors.initials: XM
authors.lastname: Chen
authors.forename: Shu-chang
authors.initials: SC
authors.lastname: Xu
authors.forename: Yi-min
authors.initials: YM
authors.lastname: Mao
authors.forename: Zhu-ping
authors.initials: ZP
authors.lastname: Fan
authors.forename: Zhi-rong
authors.initials: ZR
authors.lastname: Wang
authors.forename: Ai-ping
authors.initials: AP
authors.lastname: Cao
mesh_terms: D000818:Animals; D008099:Liver / Q000166:cytology*; D008103:Liver Cirrhosis / Q000209:etiology* / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D018160:Receptors, Cytoplasmic and Nuclear / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology*
chemical_list: D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors