[Biological characteristics of female rabbit clitoral smooth muscle cell culture in vitro].
OBJECTIVE
To investigate the method of culturing rabbit clitoral smooth muscle cells(SMC) in vitro and their biological characteristics.


METHODS
Rabbit clitoral SMCs were cultured in vitro by enzymatically dispersed method, and morphological observation, cell counting and immunohistochemical staining were employed to study their biological characteristics.


RESULTS
Rabbit clitoral SMCs were spindle-shaped and parallelled along their longitudinal axis, and the attachment and proliferation in vitro were rapid.


CONCLUSIONS
Rabbit clitoral SMCs cultured in vitro can grow and maintain their steady characteristics when provided with appropriate culture condition. Clitoral SMCs cultured in vitro can serve as a cell material for researches of clitoris.
Liu
Bo
B
Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Liu
Jihong
J
Wang
Tao
T
D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D002987:Clitoris / Q000166:cytology*; D005260:Female; D007150:Immunohistochemistry; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D011817:Rabbits