title: Subtracted restriction fingerprinting--a new typing technique using magnetic capture of tagged restriction fragments.
abstract: Molecular typing of bacterial pathogens is an important issue in the epidemiological analysis of emerging infections in humans and animals. Numerous methods have been developed for and applied to a wide variety of bacteria of medical, veterinary and zoonotic importance. The present minireview provides a description of a new typing approach designated subtracted restriction fingerprinting (SRF), its use for typing of Salmonella isolates and a comparison with the most widely used typing techniques for these bacteria. SRF is based on double restriction endonuclease digestion of whole cell DNA, followed by a fill-in reaction with specifically tagged nucleotides and subtractive capture of selected restriction fragments. This results in a reduced number of fragments optimal for separation in standard agarose gels.
authors.affiliation: Institute for Animal Breeding, Federal Agricultural Research Center (FAL), Höltystrasse 10, 31535 Neustadt-Mariensee, Germany.
authors.forename: Valeri
authors.initials: V
authors.lastname: Terletski
authors.forename: Geovana Brenner
authors.initials: GB
authors.lastname: Michael
authors.forename: Stefan
authors.initials: S
authors.lastname: Schwarz
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000191:economics / Q000379:methods*; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012475:Salmonella / Q000737:chemistry* / Q000145:classification; D012480:Salmonella Infections / Q000382:microbiology*; D012481:Salmonella Infections, Animal / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial