Synthetic peptide immunocortin stimulates the production of 11-oxycorticosteroides by rat adrenal cortex through ACTH receptors.
Adrenocorticotropic hormone (ACTH)-like peptide immunocortin (IMC) VKKPGSSVKV, corresponding to the amino acid sequence 11-20 of the variable part of human immunoglobulin G (IgG) 1 heavy chain, at concentrations of 10(-9)-10(-6) I was found to increase the adenylate cyclase activity in adrenal cortex membranes, while intramuscular injection of immunocortin at doses of 10-100 microg/kg was found to stimulate the secretion of 11-oxycorticosteroids (CS) from the adrenals to the bloodstream. Immunocortin was labeled with tritium to specific activity of 22 Ci/mmol. Receptor binding studies revealed that [(3)H]immunocortin ([(3)H]IMC) bound with high affinity and specificity to ACTH receptors on rat adrenal cortex membranes (K(d)=2.1+/-0.2 nM, B(max)=1.1+/-0.1 pmol/mg protein).
Navolotskaya
E V
EV
Branch of Shemyakin and Ovchinnikov, Institute of Bioorganic Chemistry, Russian Academy of Sciences, Science Avenue, 6, 142290 Pushchino, Moscow Region, Russia. navolots@fibkh.serpukhov.su
Vanina
V I
VI
Zolotarev
Yu A
YA
Kudryashova
N Yu
NY
Goncharenko
E N
EN
Kolobov
A A
AA
Kampe-Nemm
E A
EA
Yurovsky
V V
VV
Lipkin
V M
VM
D015062:11-Hydroxycorticosteroids / Q000378:metabolism*; D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism*; D000311:Adrenal Glands / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D007074:Immunoglobulin G / Q000737:chemistry* / Q000378:metabolism; D007135:Immunoglobulin Variable Region / Q000737:chemistry; D007700:Kinetics; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D051381:Rats; D018043:Receptors, Corticotropin / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014316:Tritium / Q000737:chemistry
D015062:11-Hydroxycorticosteroids; D007074:Immunoglobulin G; D007135:Immunoglobulin Variable Region; D010446:Peptide Fragments; D010455:Peptides; D018043:Receptors, Corticotropin; D014316:Tritium; C082551:immunocorticotropin; D000242:Cyclic AMP