Molecularly imprinted copolymer membranes functionalized by phase inversion imprinting for uracil recognition and permselective binding.
Uracil (URA) was selected as a template for preparing molecularly imprinted membranes of poly(acrylonitrile-co-methylacrylic acid) [P(AN-co-MAA)] using the phase inversion technique. This study used Fourier transform infra-red (FT-IR) and (1)H nuclear magnetic resonance (NMR) spectroscopic studies to characterize the polymer-template interaction and scanning electron microscopy (SEM) and atomic force microscopy (AFM) for morphology of the URA imprinted membrane. Resultant membranes had typical ultrafiltration structure with porous morphology and showed a permeation flux of 3.5 x 10 9-5)m(3)/(m(2)s) for 32 microM URA aqueous solution. Permselective binding to the target molecule was observed in permeation experiments with 7.9 micromol/g binding capacity of URA. Binding selectivity was discussed for URA and its analogs, dimethyluracil (DMURA) and caffeine (CAF), with 0.6 and 0.8 micromol/g binding capacity, respectively.
Wang
Hong Ying
HY
Material Engineering School of Zhengzhou University, Zhengzhou 450052, PR China.
Xia
Shao Ling
SL
Sun
Hong
H
Liu
Yu Kun
YK
Cao
Shao Kui
SK
Kobayashi
Takaomi
T
D001665:Binding Sites; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry*; D014498:Uracil / Q000032:analysis* / Q000737:chemistry
D008567:Membranes, Artificial; D011108:Polymers; D014498:Uracil