title: Effect of dentin perfusion on the sealing ability and microtensile bond strengths of a total-etch versus an all-in-one adhesive.
abstract: OBJECTIVE
  To test the null hypothesis that perfusion of dentin has no effect on the sealing or bond strength in Class V resin composite restorations using a two-step total-etch (Scotchbond 1), or a one-step self-etch (all-in-one) (Prompt L-Pop) adhesive.
  
  
  METHODS
  Dentin permeability in Class V cavity preparations cut into extracted human third molars was measured in a fluid transport setup. Resin composites were placed using either Scotchbond 1, or Prompt L-Pop with or without dentin perfusion at 1.47 kPa. Permeability was re-measured, and the reduction in permeability was calculated as a percentage of the initial value. Additionally, microtensile bond strength (microTBS) to dentin was measured.
  
  
  RESULTS
  Whereas the ability of Scotchbond 1 to seal dentin was significantly decreased due to dentin perfusion (p = 0.00025), that of Prompt L-Pop was not affected (p = 0.099). Scotchbond 1 produced significantly less dentinal seal than Prompt L-Pop only in the restorations carried out with perfusion (p = 0.0003), in the absence of perfusion, however, the difference was not significant (p = 0.343). Dentin perfusion significantly decreased the microTBS of Scotchbond 1 to dentin (p = 0.001). Due to excessive premature failure during sectioning, microTBS test could not be performed with Prompt L-Pop.
  
  
  CONCLUSIONS
  Dentinal fluid flow may have detrimental effects on the sealing ability of dental adhesives depending on the type of adhesive used. Studies aiming to rank adhesives in relation to their sealing ability in vitro should not disregard dentin perfusion.
authors.affiliation: Department of Cariology Endodontology Pedodontology, Academic Center for Dentistry Amsterdam (ACTA), Louwesweg 1, Amsterdam, 1066 EA, Netherlands. r.ozok@acta.nl
authors.forename: Ahmet R
authors.initials: AR
authors.lastname: Ozok
authors.forename: Min-Kai
authors.initials: MK
authors.lastname: Wu
authors.forename: Anton J
authors.initials: AJ
authors.lastname: De Gee
authors.forename: Paul R
authors.initials: PR
authors.lastname: Wesselink
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D001840:Dental Bonding; D003737:Dental Cavity Preparation; D003763:Dental Leakage / Q000517:prevention & control; D003793:Dental Restoration, Permanent / Q000379:methods; D003799:Dental Stress Analysis; D003806:Dentin Permeability; D017220:Dentin-Bonding Agents; D019234:Dentinal Fluid / Q000502:physiology*; D006801:Humans; D008422:Materials Testing; D008963:Molar; D010477:Perfusion; D019279:Resin Cements; D013718:Tensile Strength
chemical_list: D017220:Dentin-Bonding Agents; C408584:Prompt L-Pop; D019279:Resin Cements; C041330:Scotchbond