title: Spatial and temporal localization of secretory IgA in healing tooth extraction sockets in a rabbit model.
abstract: OBJECTIVE
  The purpose of this study was to look at the spatial and temporal localization of secretory IgA in healing tooth extraction sockets in a rabbit model.
  
  
  METHODS
  Twenty-four male New Zealand White rabbits were used in the study. Incisor teeth were extracted from both jaws, and the healing extraction socket with the surrounding jaw bone was harvested at 48 hours, 4 days, and 1, 2, 4, 8, 12, and 16 weeks. Tissues were fixed, decalcified, and processed for hematoxylin and eosin and immunohistochemical staining. The sections were stained to detect secretory IgA. The stained sections were then imaged, and an automated computer program was used to detect the brown 3,3'-diaminobenzidine stain that represented the secretory IgA. The data were obtained in the form of percentage area and intensity of stain and analyzed using analysis of variance (Tukey-Kramer and Scheffé's tests).
  
  
  RESULTS
  Spatial and temporal differences in localization of secretory IgA were observed across time frames in both jaws.
  
  
  CONCLUSIONS
  The results of this study showed definite trends in the spatial and temporal localization of secretory IgA in healing tooth extraction sockets in a rabbit model.
authors.affiliation: Department of Oral and Maxillofacial Surgery, The University of Texas Health Science Center at Houston, Houston, TX, USA. Zahid.Lalani@uth.tmc.edu
authors.forename: Zahid
authors.initials: Z
authors.lastname: Lalani
authors.forename: Mark
authors.initials: M
authors.lastname: Wong
authors.forename: Eric M
authors.initials: EM
authors.lastname: Brey
authors.forename: Antonios G
authors.initials: AG
authors.lastname: Mikos
authors.forename: P J
authors.initials: PJ
authors.lastname: Duke
authors.forename: Carol
authors.initials: C
authors.lastname: Johnston
mesh_terms: D000539:Alveolar Process / Q000276:immunology; D000704:Analysis of Variance; D000818:Animals; D005109:Extracellular Matrix / Q000276:immunology; D007091:Image Processing, Computer-Assisted; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D007150:Immunohistochemistry; D008297:Male; D023421:Models, Animal; D011817:Rabbits; D013997:Time Factors; D014081:Tooth Extraction; D020390:Tooth Socket / Q000276:immunology*; D014945:Wound Healing / Q000276:immunology*
chemical_list: D007071:Immunoglobulin A, Secretory