title: [Prophylactic effect of Lactobacillus GG in animal colitis and its effect on cytokine secretion and mucin gene expressions].
abstract: OBJECTIVE
  Lactobacillus rhamnosus GG (LGG) has been used in acute colitis treatment. However, it is unclear whether the LGG prevents chronic colitis. The aim of this study was to examine the prophylactic effect of LGG on animal colitis, cytokine secretion, and mucin gene expression.
  
  
  METHODS
  BALB/c mice (n=64) were exposed to 5% dextran sulfate sodium (DSS) for 7 days followed by 10 days recovery period and repeatedly exposed for 4 days. Then, the mice were devided into three group; group of oral LGG adminstration throughout the recovery and repeated colitis period; PBS group of PBS administration; control group. Colon length, histologic score, tumor necrosis factor-alpha (TNF-alpha), interleukin-10 (IL-10) levels, mucin gene expressions were determined at each period.
  
  
  RESULTS
  In acute colitis period, the LGG group showed higher levels of disease activity index (DAI), histologic score, TNF-alpha, IL-10, but shorter colon length, lower levels of mucin gene expressions than the control group. However, in repeated colitis period, the LGG group showed markedly lower levels of DAI and IL-10 but significantly longer colon length than PBS group (p<0.05). There was no difference in the mucin gene expression.
  
  
  CONCLUSIONS
  These results suggest that LGG prevents chronic murine colitis. It may be associated with cytokine modulation and competitive inhibition of pathogenic bacteria. However, it may not be related with gene expression.
authors.affiliation: Department of Internal Medicine, University of Ulsan College of Medicine, Asan Medical Center, 388-1 Pungnap-2dong, Songpa-gu, Seoul 138-736, Korea. sjm5675@amc.seoul.kr
authors.forename: Gyoo
authors.initials: G
authors.lastname: Moon
authors.forename: Seung-Jae
authors.initials: SJ
authors.lastname: Myung
authors.forename: Jin-Yong
authors.initials: JY
authors.lastname: Jeong
authors.forename: Suk-Kyun
authors.initials: SK
authors.lastname: Yang
authors.forename: Yoon Kyung
authors.initials: YK
authors.lastname: Cho
authors.forename: Sun-Mi
authors.initials: SM
authors.lastname: Lee
authors.forename: Hye-Sook
authors.initials: HS
authors.lastname: Chang
authors.forename: Jeong-Sik
authors.initials: JS
authors.lastname: Byeon
authors.forename: Yun Jung
authors.initials: YJ
authors.lastname: Lee
authors.forename: Gin Hyug
authors.initials: GH
authors.lastname: Lee
authors.forename: Weon-Seon
authors.initials: WS
authors.lastname: Hong
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Young Il
authors.initials: YI
authors.lastname: Min
authors.forename: Jung-Sun
authors.initials: JS
authors.lastname: Kim
mesh_terms: D000818:Animals; D003092:Colitis / Q000517:prevention & control*; D016207:Cytokines / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects*; D007778:Lactobacillus; D051379:Mice; D008807:Mice, Inbred BALB C; D009077:Mucins / Q000235:genetics* / Q000378:metabolism; D019936:Probiotics / Q000627:therapeutic use*
chemical_list: D016207:Cytokines; D009077:Mucins