title: Efficient generation of antileukemic autologous T cells by short-term culture and gamma-irradiation of myeloid leukemic cells.
abstract: Blasts from patients with acute myeloid leukemia (AML) can be differentiated in dendritic cells (DCs) using appropriate combinations of cytokines. However, generation of autologous antileukemic cytotoxic T cells using leukemic DCs remains difficult. We have previously reported that expression of costimulatory molecules in cultured AML cells could be induced by gamma-irradiation. In the present study, blasts from 21 patients with AML were cultured in vitro for 2 days, then cells were gamma-irradiated and antigen-presenting cell (APC) characteristics were assessed. gamma-Irradiation induced expression of several characteristics of APCs in AML blasts, including expression of CD80, CD86, and BDCA-4, and were stimulators of allogeneic mixed lymphocyte reactions. Autologous antileukemic cytotoxicity was induced in seven out of ten cases. This study shows that cells with APC characteristics and able to induce ex vivo stimulation of autologous antileukemic T cells can be generated from AML cells using the simple and rapid method of gamma-irradiation of cultured leukemic cells.
authors.affiliation: Unité INSERM 524, Institut de Recherche sur le Cancer de Lille, Lille, France.
authors.forename: Rodolphe
authors.initials: R
authors.lastname: Vereecque
authors.forename: Aurore
authors.initials: A
authors.lastname: Saudemont
authors.forename: Stéphane
authors.initials: S
authors.lastname: Depil
authors.forename: Selim
authors.initials: S
authors.lastname: Corm
authors.forename: Joris
authors.initials: J
authors.lastname: Andrieux
authors.forename: Valérie
authors.initials: V
authors.lastname: Soenen-Cornu
authors.forename: Bruno
authors.initials: B
authors.lastname: Quesnel
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D000938:Antigen-Presenting Cells / Q000276:immunology*; D015703:Antigens, CD / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen; D002478:Cells, Cultured / Q000276:immunology / Q000528:radiation effects; D005260:Female; D005720:Gamma Rays; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D007951:Leukemia, Myeloid / Q000276:immunology* / Q000473:pathology; D008213:Lymphocyte Activation; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D015703:Antigens, CD; D000951:Antigens, Neoplasm; D001324:Autoantigens; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497964:CD86 protein, human; D008562:Membrane Glycoproteins