Protein extraction and comparison of stain protocols for analysis of two-dimensional electrophoresis gels.
Protein extraction and the proteome of Lactobacillus delbrueckii subsp. bulgaricus were studied using different stains. The reversible silver staining technique was shown to be more sensitive than the irreversible silver stain. Coomassie colloidal was demonstrated to be as sensitive as reversible silver stain; however, the Coomassie colloidal blue solution developed a higher background and for sample preparation was more time-consuming.
Silva
Joana
J
Escola Superior de Biotecnologia, Universidade Catolica Portuguesa, Rua Dr. Antonio Bernardino de Almeida, 4200-072 Porto, Portugal.
Carvalho
Ana Sofia
AS
Vitorino
Rui
R
Domingues
Pedro
P
Teixeira
Paula
P
Gibbs
Paul
P
D001426:Bacterial Proteins / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods / Q000592:standards; D007202:Indicators and Reagents; D007778:Lactobacillus / Q000737:chemistry; D012394:Rosaniline Dyes; D016622:Silver Staining
D001426:Bacterial Proteins; D007202:Indicators and Reagents; D012394:Rosaniline Dyes; C048139:Coomassie blue