title: Nonmyeloablative bone marrow transplantation of BXSB lupus mice using fully matched allogeneic donor cells from green fluorescent protein transgenic mice.
abstract: Male BXSB mice, a mouse model of systemic lupus erythematosus, were given bone marrow transplants (BMT) at 20 wk of age using MHC-matched donor cells and nonmyeloablative conditioning (550 cGy irradiation). Transplanted mice and irradiation controls were followed for a period of 20 wk. Mice transgenic for green fluorescent protein were used as donors to allow tracking of donor cells and a determination of chimerism. Radiation controls had reduced renal pathology at 10 wk posttransplant, but not at 20 wk compared with untreated mice, while nonmyeloablative BMT mice had significantly reduced pathology at both time intervals. The monocytosis characteristic of older BXSB mice was also reduced by BMT, but the treatment did not prevent production of Ab to dsDNA. A stable chimerism of 24-40% donor CD45-positive cells was achieved in spleen and bone marrow, and there was no evidence of clinical graft vs host disease. Donor cells were detected in most recipient organs, notably the thymus and renal glomeruli. The results suggest that complete depletion of mature lymphocytes or of progenitor stem cells is not required to control lupus nephritis in BXSB mice.
authors.affiliation: Pediatric Rheumatology, All Children's Hospital, University of South Florida, St. Petersburg, FL 33701, USA. joneso@allkids.org
authors.forename: Olcay Y
authors.initials: OY
authors.lastname: Jones
authors.forename: Ann
authors.initials: A
authors.lastname: Steele
authors.forename: Joe M
authors.initials: JM
authors.lastname: Jones
authors.forename: Yasmin
authors.initials: Y
authors.lastname: Marikar
authors.forename: Yenhui
authors.initials: Y
authors.lastname: Chang
authors.forename: Alexander
authors.initials: A
authors.lastname: Feliz
authors.forename: Richard A
authors.initials: RA
authors.lastname: Cahill
authors.forename: Robert A
authors.initials: RA
authors.lastname: Good
mesh_terms: D000818:Animals; D000974:Antibodies, Antinuclear / Q000096:biosynthesis; D016026:Bone Marrow Transplantation / Q000276:immunology* / Q000379:methods / Q000473:pathology; D004247:DNA / Q000276:immunology; D005260:Female; D049452:Green Fluorescent Proteins; D006650:Histocompatibility Testing / Q000379:methods; D007668:Kidney / Q000473:pathology; D007964:Leukocytosis / Q000235:genetics / Q000276:immunology / Q000473:pathology; D008164:Luminescent Proteins / Q000235:genetics*; D008180:Lupus Erythematosus, Systemic / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D009000:Monocytes / Q000276:immunology / Q000473:pathology; D011828:Radiation Chimera / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology / Q000473:pathology; D019172:Transplantation Conditioning / Q000379:methods
chemical_list: D000974:Antibodies, Antinuclear; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D004247:DNA