title: CLAG 9 is located in the rhoptries of Plasmodium falciparum.
abstract: Clag 9, a gene located on chromosome 9 of Plasmodium falciparum has previously been associated with the cytoadherence of parasitized erythrocytes to CD36. This gene is part of a multi-gene family found in all Plasmodium species studied to date. Using data from the Malaria Genome Sequencing Project, peptides specific for clag 9 were designed, synthesized and used to immunize mice. This antisera was used in Western blotting and immunofluorescence experiments to determine the cellular localization of CLAG 9 in the parasitized erythrocyte. Co-localization using immunofluorescence of wildtype and knockout parasites unequivocally shows that CLAG 9 is localized to the rhoptry organelles of P. falciparum.
authors.affiliation: Malaria Biology Laboratory, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research, 300 Herston Road, 4006 Herston, Queensland, Australia. donG@qimr.edu.au
authors.forename: Donald L
authors.initials: DL
authors.lastname: Gardiner
authors.forename: Tobias
authors.initials: T
authors.lastname: Spielmann
authors.forename: Matthew W A
authors.initials: MW
authors.lastname: Dixon
authors.forename: Paula L
authors.initials: PL
authors.lastname: Hawthorne
authors.forename: Maria R
authors.initials: MR
authors.lastname: Ortega
authors.forename: Karen L
authors.initials: KL
authors.lastname: Anderson
authors.forename: Tina S
authors.initials: TS
authors.lastname: Skinner-Adams
authors.forename: David J
authors.initials: DJ
authors.lastname: Kemp
authors.forename: Katharine R
authors.initials: KR
authors.lastname: Trenholme
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D004912:Erythrocytes / Q000469:parasitology; D005455:Fluorescent Antibody Technique; D006801:Humans; D016778:Malaria, Falciparum / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D015388:Organelles / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D010963:Plasmodium falciparum / Q000235:genetics / Q000378:metabolism*; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: D000913:Antibodies, Protozoan; D015815:Cell Adhesion Molecules; D010455:Peptides; D015800:Protozoan Proteins; C407823:clag9 protein, Plasmodium falciparum