Development of cell cultures with competency for contributing to the zebrafish germ line.
The zebrafish is an established model for the genetic analysis of vertebrate development. Forward-genetic screens have generated thousands of mutations, and antisense-based methods have been used to transiently knockdown gene expression during embryogenesis. Although these methods have made the zebrafish a valuable system for the identification and functional characterization of developmentally important genes, one deficiency of the zebrafish model is the absence of methods to introduce targeted mutations to generate knockout lines of fish. Application of gene-targeting methods has been limited in nonmurine species due to the absence of germ-line competent embryonic stem (ES) cell lines. Recently, progress was made in addressing this problem by the derivation of zebrafish embryo cell lines that remain pluripotent and germ-line competent for multiple passages in culture. Zebrafish germ-line chimeras were generated using cultures derived from embryos at two different developmental stages, and targeted insertion of vector DNA by homologous recombination was demonstrated in both cultures. Several strategies are being used to optimize the production and identification of germ-line chimeras. The zebrafish embryo cell culture system should provide the basis of a gene-targeting approach that will complement other genetic strategies and improve the utility of the zebrafish model for studies of development and disease.
Fan
Lianchun
L
Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA.
Alestrom
Annette
A
Alestrom
Peter
P
Collodi
Paul
P
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D004625:Embryo, Nonmammalian / Q000166:cytology; D018390:Gene Targeting / Q000379:methods*; D005854:Germ Cells / Q000166:cytology*; D016254:Mutagenesis, Insertional; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*