Methods for subchoroidal implantation of Greene melanoma in rabbits.
As an alternative to previously reported treatments, experimental animal models of uveal melanoma provide useful information for assessing the advantage of treatments designed to salvage eyes. Greene melanoma is the best-established model and has been used for the purpose of assessment of several treatments. Various other experimental models that implant melanoma cell lines into the anterior chamber of the rabbit eye have been reported. This article reviews some of the advantages and disadvantages of these experimental models of human uveal melanoma in rabbits and presents a modified subchoroidal implantation procedure for melanoma cells that is used in our laboratory. This procedure effectively, efficiently, and consistently enables us to provide animal models bearing large-sized choroidal melanomas. This modified technique may eventually be applicable to the development of new therapeutic options.
Shikishima
Keigo
K
Department of Ophthalmology, Aoto Hospital, Jikei University School of Medicine, 6-41-2 Aoto, Katsushika-ku, 25-0062, Tokyo, Japan. shiki@jikei.ac.jp
D000818:Animals; D006224:Cricetinae; D008545:Melanoma / Q000473:pathology*; D023421:Models, Animal; D009368:Neoplasm Transplantation; D011817:Rabbits; D014604:Uveal Neoplasms / Q000473:pathology*