The relationship between the degree of thrombocytopenia and infection with Ehrlichia canis in an endemic area.
Ehrlichia canis is the causative agent of canine monocytic ehrlichiosis. In order to evaluate platelet counts as a screening test for E. canis in an endemic area, 217 whole blood samples from dogs were divided into three groups: 71 non-thrombocytopenic samples (group A, platelet counts greater than 200 000/mL) and 146 thrombocytopenic samples (less than 200 000/mL). The thrombocytopenic group was further divided into 62 with platelet counts between 100 000-200 000/mL (Group B) and 84 samples with less than 100 000 platelets/mL (Group C). All samples were examined for the presence of a segment of the Ehrlichia canis 16S rRNA gene using a nested polymerase chain reaction. Sixty-seven of the 217 samples (30.9%) were positive for the presence of the E. canis 16S rRNA gene; 53 (63.1%) of the group C samples and 13 (21%) of group B. Only one (1.4%) of the non-thrombocytopenic samples (Group A) was positive. These data support the concept that platelet counts may be a good screening test for canine monocytic ehrlichiosis, and that the magnitude of thrombocytopenia may increase the reliability of diagnosis.
Bulla
Camilo
C
Clinical Pathology Laboratory, Departamento de Clínica Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, Brazil. cbulla@fmvz.unesp.br
Kiomi Takahira
Regina
R
Pessoa Araújo
João
J
AparecidaTrinca
Luzia
L
Souza Lopes
Raimundo
R
Wiedmeyer
Charles Edward
CE
D000818:Animals; D004283:Dog Diseases / Q000175:diagnosis* / Q000276:immunology; D004285:Dogs; D041061:Ehrlichia canis / Q000235:genetics / Q000302:isolation & purification; D016873:Ehrlichiosis / Q000150:complications / Q000175:diagnosis / Q000662:veterinary*; D008403:Mass Screening / Q000379:methods / Q000662:veterinary*; D010976:Platelet Count / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D012336:RNA, Ribosomal, 16S / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013921:Thrombocytopenia / Q000175:diagnosis / Q000209:etiology / Q000662:veterinary*
D012336:RNA, Ribosomal, 16S