title: Myosin light chain composition in non-failing donor and end-stage failing human ventricular myocardium.
abstract: The increased Ca(2+)-responsiveness in end-stage human heart failure cannot be attributed to contractile protein isoform changes, but rather is the complex resultant of changes in degree of phosphorylation of VLC-2 and TnI. Despite the decreased basal level of VLC-2 phosphorylation the response to VLC-2 dephosphorylation is enhanced in failing myocytes, which might result from differences in endogenous phosphorylation of thin and thick filament proteins between donor and failing hearts. Taken together decreased VLC-2 phosphorylation in end-stage human heart failure might represent a compensatory process leading to an improvement of myocardial contractility by opposing the detrimental effects of increased Ca(2+)-responsiveness of force and impaired Ca(2+)-handling on diastolic function.
authors.affiliation: Laboratory for Physiology, Institute for Cardiovascular Research, VU University Medical Center, Amsterdam, the Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: van der Velden
authors.forename: Z
authors.initials: Z
authors.lastname: Papp
authors.forename: N M
authors.initials: NM
authors.lastname: Boontje
authors.forename: R
authors.initials: R
authors.lastname: Zaremba
authors.forename: J W
authors.initials: JW
authors.lastname: de Jong
authors.forename: P M L
authors.initials: PM
authors.lastname: Janssen
authors.forename: G
authors.initials: G
authors.lastname: Hasenfuss
authors.forename: G J M
authors.initials: GJ
authors.lastname: Stienen
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D006333:Heart Failure / Q000473:pathology; D006352:Heart Ventricles / Q000473:pathology*; D006801:Humans; D008954:Models, Biological; D008956:Models, Chemical; D009206:Myocardium / Q000166:cytology*; D018994:Myosin Light Chains / Q000737:chemistry*; D010766:Phosphorylation; D020033:Protein Isoforms; D011720:Pyrazoles / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism
chemical_list: C108123:4-amino-5-(4-methylphenyl)-7-(tert-butyl)pyrazolo(3,4-d)pyrimidine; D018994:Myosin Light Chains; D020033:Protein Isoforms; D011720:Pyrazoles; D011743:Pyrimidines; D002118:Calcium