Astilbin prevents concanavalin A-induced liver injury by reducing TNF-alpha production and T lymphocytes adhesion.
The aim of this study was to evaluate the effect of astilbin on concanavalin A (Con A)-induced hepatitis, a T cell-dependent model of liver injury. Con A administration resulted in a severe liver injury in mice, with a strong increment in spleen cell adhesion and liver infiltration of T cells, as well as in tumour necrosis factor (TNF)-alpha production. Against this liver injury, astilbin significantly inhibited the elevation in transaminase activity, reduced the TNF-alpha production, and improved the histological changes, including inflammatory infiltration, hepatocyte necrosis and degeneration and Kupffer cell hyperplasia. In addition, astilbin inhibited the adhesion of spleen cells and purified T lymphocytes isolated from the liver-injured mice to fibronectin, laminin and type IV collagen.Moreover, the adhesion of human Jurkat T cells to endothelial cell line ECV-304 was also inhibited by astilbin. These results suggest that the improvement of the T cell-mediated liver injury by astilbinmay be related to the reduction in TNF-alpha production and in T cell adhesion to extracellular matrices and endothelial cells.
Wang
Jun
J
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, China.
Zhao
Ying
Y
Xu
Qiang
Q
D000818:Animals; D002448:Cell Adhesion; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D024141:Collagen Type IV / Q000502:physiology; D003208:Concanavalin A; D005109:Extracellular Matrix / Q000502:physiology; D005260:Female; D005353:Fibronectins / Q000502:physiology; D044948:Flavonols / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D019169:Jurkat Cells; D007797:Laminin / Q000502:physiology; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000737:chemistry; D032281:Smilax; D013601:T-Lymphocytes / Q000187:drug effects* / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000096:biosynthesis
D024141:Collagen Type IV; D005353:Fibronectins; D044948:Flavonols; D007797:Laminin; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D003208:Concanavalin A; C099069:astilbin