Towards sorting of biolibraries using single-molecule fluorescence detection techniques.
The selection of specific binding molecules like peptides and proteins from biolibraries using, for instance, phage display methods can be quite time-consuming. It is therefore desirable to develop a strategy that is much faster in selection and sorting of potential binders out of a biolibrary. In this contribution we separately discuss the current achievements in generation of biolibraries, single-molecule detection techniques and microfluidic devices. A high-throughput microfluidic platform is then proposed that combines the propulsion of liquid containing fluorescent components of the biolibrary through microchannels, single-molecule fluorescence photon burst detection and real-time sorting of positive hits.
Visser
Antonie J W G
AJ
MicroSpectroscopy Centre, Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands. Ton.Visser@wur.nl
Kunst
Beno H
BH
Keller
Hans
H
Schots
Arjen
A
D005453:Fluorescence; D005544:Forecasting; D015723:Gene Library; D007834:Lasers; D044085:Microfluidics / Q000295:instrumentation; D019151:Peptide Library; D013050:Spectrometry, Fluorescence / Q000379:methods
D019151:Peptide Library