title: The mouse natural killer T cell-associated antigen recognized by U5A2-13 monoclonal antibody is intercellular adhesion molecule-1.
abstract: Natural Killer T (NKT) cells in mice are generally defined as NK1.1(+) T cells, although NK1.1 antigen is expressed only in C57BL/6 and related strains. This has precluded investigations of other strains. To find a novel NKT cell surface marker, we generated a monoclonal antibody (mAb), U5A2-13, which recognizes phenotypically and functionally similar populations to NKT cells in naïve mice irrespective of strain. Here, by using a COS-7 expressional cloning system, we molecularly cloned a cDNA encoding a protein reactive with the U5A2-13 mAb and then identified it as intercellular adhesion molecule-1 (ICAM-1). Importantly, the U5A2-13 mAb did not stain hepatic mononuclear cells from ICAM-1 gene disrupted mice. Furthermore, Pepscan method disclosed that the discontinuous epitope for U5A2-13 mAb is composed of three loops located in extracellular domain two of ICAM-1. Overall, U5A2-13, a mAb originally established for mouse NKT cells, recognizes a novel conformational epitope of ICAM-1.
authors.affiliation: Pharmacology Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-ku, Tokyo 104-0045, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Shimizu
authors.forename: Hiroki
authors.initials: H
authors.lastname: Sasaki
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Aoyagi
authors.forename: Mitsuzi
authors.initials: M
authors.lastname: Yoshida
authors.forename: Kazunori
authors.initials: K
authors.lastname: Kato
authors.forename: Yuji
authors.initials: Y
authors.lastname: Heike
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Ikarashi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shirakawa
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Takaue
authors.forename: Atsushi
authors.initials: A
authors.lastname: Miyajima
authors.forename: Masaaki
authors.initials: M
authors.lastname: Terada
authors.forename: Hideo
authors.initials: H
authors.lastname: Nagai
authors.forename: Hiro
authors.initials: H
authors.lastname: Wakasugi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003001:Cloning, Molecular; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005260:Female; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D018984:Epitopes, T-Lymphocyte; D018799:Intercellular Adhesion Molecule-1