title: Biochemical bone turnover markers are useful tools to assess changes in bone metabolism in marmosets.
abstract: This study was designed to investigate whether biochemical markers of bone resorption and formation could be determined in the serum and urine of marmosets (Callithrix jacchus), using standard laboratory chemistry methods and commercially available human kits. Consequently, the findings from this study will indicate whether the techniques and kits could serve as appropriate tools for assessing changes in bone turnover in this species. Two groups of animals (n = 12/group), consisting of a comparable number of young and old male and female marmosets, were given either isotonic saline or a single dose of the bisphosphonate ibandronate (0.1 mg/kg) s.c. in order to suppress bone turnover. Blood and urine were collected at baseline and 5 days after administration. Samples were analyzed for urinary (u) and serum (s) markers of bone formation (serum osteocalcin [sOC], serum N-terminal crosslinks of human pro-collagen type I [sP1NP]) and bone resorption (urinary pyridinoline [uPYD], urinary deoxypyridinoline [uDPD], serum C-terminal crosslinks of human collagen type I (C-telopeptide) [sCTX]), intact serum parathyroid hormone (iPTH) and urinary calcium and creatinine. Levels of all the markers of bone resorption and formation decreased during the study period. As expected, the bone formation markers decreased slightly less relative to the resorption markers. The most sensitive markers were sCTX (-33%; P < or =0.001) for bone resorption, and sP1NP (-3%; P < or =0.05) for bone formation. Serum PTH levels increased by 8% ( P < or =0.05), demonstrating a physiological reaction to prevent changes in serum calcium. Although not all variables reached statistical significance within the tested interval, the applied methods and kits were considered suitable for evaluating bone turnover changes in marmosets. Thus, these methods and kits can be utilized not only during the course of pharmacological investigations but also as additional tools to assess the overall bone health of this species.
authors.affiliation: Department of Pharma Research, Animal Husbandry, F. Hoffmann-La Roche Ltd., Basel, Switzerland.
authors.forename: Anna
authors.initials: A
authors.lastname: Angeliewa
authors.forename: Michael
authors.initials: M
authors.lastname: Budde
authors.forename: Manfred
authors.initials: M
authors.lastname: Schlachter
authors.forename: Nicholas R
authors.initials: NR
authors.lastname: Hoyle
authors.forename: Frieder
authors.initials: F
authors.lastname: Bauss
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001862:Bone Resorption / Q000378:metabolism*; D001842:Bone and Bones / Q000378:metabolism*; D002144:Callithrix / Q000378:metabolism*; D005260:Female; D008297:Male; D015999:Multivariate Analysis; D014659:Vasectomy
chemical_list: D015415:Biomarkers