Factorial design, physicochemical characterisation and activity of ciprofloxacin-PLGA nanoparticles.
Poly(lactide-co-glycolide) nanoparticles incorporating ciprofloxacin HCl were prepared by means of a W/O/W emulsification solvent evaporation method. The stabiliser selected was poly(vinylalcohol). A 2(4) full factorial design based on four independent variables was used to plan the experiments and the variable parameters were the number of homogenisation cycles, addition of boric acid to the inner water phase containing the drug, ciprofloxacin concentration in the inner water phase and oil:outer water phase ratio. The effects of these parameters on the particle size, zeta potential, drug loading efficiency and drug release were investigated. Also the effect of gamma irradiation on the particle size and drug release was evaluated and DSC and XRD analyses of the compounds and the nanoparticles were performed. The activity on two series of microorganisms, Pseudomonas aeruginosa and Staphylococcus aureus, was examined.
Dillen
Kathleen
K
Laboratory of Pharmaceutical Technology and Biopharmacy, Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp (Wilrijk), Belgium.
Vandervoort
Jo
J
Van den Mooter
Guy
G
Verheyden
Loes
L
Ludwig
Annick
A
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002939:Ciprofloxacin / Q000737:chemistry* / Q000494:pharmacology; D003460:Crystallization; D004339:Drug Compounding; D005163:Factor Analysis, Statistical; D005720:Gamma Rays; D019344:Lactic Acid / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D036103:Nanotechnology; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012996:Solutions; D013211:Staphylococcus aureus / Q000187:drug effects; D013242:Sterilization; D013696:Temperature; D014961:X-Ray Diffraction
D000900:Anti-Bacterial Agents; D011108:Polymers; D012996:Solutions; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D002939:Ciprofloxacin