title: An interferon-gamma ELISPOT and immunohistochemical investigation of cytotoxic T lymphocyte-mediated tumour immunity in patients with paraneoplastic cerebellar degeneration and anti-Yo antibodies.
abstract: Eight patients with paraneoplastic cerebellar degeneration (PCD) and anti-Yo antibodies were investigated to determine whether there is any association between cytotoxic T lymphocyte (CTL) responses reactive with two previously defined Yo-derived, HLA-A2.1 restricted epitopes (cdr2-1 and cdr2-2) and the presence of tumour-infiltrating CD8+ CTLs. cdr2-1 and cdr2-2-specific CTL responses could not be detected in 5 HLA-A2.1(+) patients in an ex vivo interferon-gamma ELISPOT assay and only 2/9 tumour sections contained CD8(+) intratumoural lymphocytes suggesting a very limited role for CTL-mediated tumour immunity in this patient group, all of whom had evidence of widespread malignancy at the time of diagnosis and/or death.
authors.affiliation: Department of Neurology, Queen Elizabeth Hospital, Edgbaston, Birmingham, UK. iansutton@email.com
authors.forename: I J
authors.initials: IJ
authors.lastname: Sutton
authors.forename: J
authors.initials: J
authors.lastname: Steele
authors.forename: C O
authors.initials: CO
authors.lastname: Savage
authors.forename: J B
authors.initials: JB
authors.lastname: Winer
authors.forename: L S
authors.initials: LS
authors.lastname: Young
mesh_terms: D000912:Antibodies, Neoplasm / Q000097:blood*; D001943:Breast Neoplasms / Q000276:immunology; D002528:Cerebellar Neoplasms / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D004268:DNA-Binding Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005260:Female; D015789:HLA-A2 Antigen / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000032:analysis* / Q000378:metabolism; D018655:Lymphocyte Count; D016246:Lymphocytes, Tumor-Infiltrating / Q000737:chemistry / Q000276:immunology / Q000473:pathology; D009363:Neoplasm Proteins / Q000276:immunology*; D009419:Nerve Tissue Proteins / Q000276:immunology; D010051:Ovarian Neoplasms / Q000276:immunology; D020362:Paraneoplastic Cerebellar Degeneration / Q000175:diagnosis / Q000276:immunology* / Q000473:pathology; D020033:Protein Isoforms / Q000276:immunology; D013194:Staining and Labeling; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000473:pathology
chemical_list: D000912:Antibodies, Neoplasm; C095184:CDR2 protein, human; D004268:DNA-Binding Proteins; D018984:Epitopes, T-Lymphocyte; D015789:HLA-A2 Antigen; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D007371:Interferon-gamma