title: Protein extraction and comparison of stain protocols for analysis of two-dimensional electrophoresis gels.
abstract: Protein extraction and the proteome of Lactobacillus delbrueckii subsp. bulgaricus were studied using different stains. The reversible silver staining technique was shown to be more sensitive than the irreversible silver stain. Coomassie colloidal was demonstrated to be as sensitive as reversible silver stain; however, the Coomassie colloidal blue solution developed a higher background and for sample preparation was more time-consuming.
authors.affiliation: Escola Superior de Biotecnologia, Universidade Catolica Portuguesa, Rua Dr. Antonio Bernardino de Almeida, 4200-072 Porto, Portugal.
authors.forename: Joana
authors.initials: J
authors.lastname: Silva
authors.forename: Ana Sofia
authors.initials: AS
authors.lastname: Carvalho
authors.forename: Rui
authors.initials: R
authors.lastname: Vitorino
authors.forename: Pedro
authors.initials: P
authors.lastname: Domingues
authors.forename: Paula
authors.initials: P
authors.lastname: Teixeira
authors.forename: Paul
authors.initials: P
authors.lastname: Gibbs
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods / Q000592:standards; D007202:Indicators and Reagents; D007778:Lactobacillus / Q000737:chemistry; D012394:Rosaniline Dyes; D016622:Silver Staining
chemical_list: D001426:Bacterial Proteins; D007202:Indicators and Reagents; D012394:Rosaniline Dyes; C048139:Coomassie blue