Poly(L-lysine)-mediated immobilisation of oligonucleotides on carboxy-rich polymer surfaces.
The immobilisation efficiency of the complexes of oligonucleotide/poly(L-lysine) on two polymeric carboxy-rich surfaces, i.e. poly(styrene/maleic acid) (PSMA) and poly(styrene/maleic anhydride) (PSMAA), has been investigated using X-ray photoelectron spectroscopy, atomic force microscopy (AFM) and fluorescence-based measurements of DNA attachment. A molecularly thin layer of either electrostatically or covalently (via amide bond) bound poly(L-lysine) allows the 'switching' from COOH-based to NH(2)-based surface functionality. The results indicate that approximately 54-57% and 55-62% of the applied oligonucleotides bind to polymeric surfaces via the route of electrostatic adsorption of poly(L-lysine) and covalent bonding of poly(L-lysine), respectively. This system can be applied conveniently for the detection of nucleic acids in both disposable and reusable biosensors.
Ivanova
Elena P
EP
Industrial Research Institute Swinburne, Swinburne University of Technology, PO Box 218, Hawthorn, Vic. 3122, Australia. eivanova@swin.edu.au
Pham
Duy K
DK
Brack
Narelle
N
Pigram
Paul
P
Nicolau
Dan V
DV
D018625:Microscopy, Atomic Force; D009841:Oligonucleotides / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D013057:Spectrum Analysis
D009841:Oligonucleotides; D011137:Polystyrenes; D011107:Polylysine