title: Effect of dietary fat on early morphological intestinal adaptation in a rat with short bowel syndrome.
abstract: Among factors promoting mucosal hyperplasia after bowel resection, long-chain fatty acids may have a special role. The purpose of the present study was to evaluate the effects of high-fat diet (HFD) on early intestinal adaptation in rats with short bowel syndrome (SBS). Male Sprague-Dawley rats underwent either a bowel transection with re-anastomosis (Sham rats) or 75% small bowel resection (SBS rats). Animals were randomly assigned to one of three groups: Sham rats fed normal chow (Sham-NC); SBS rats fed NC (SBS-NC); and SBS rats fed HFD (SBS-HFD). Rats were killed on days 3 or 14. Body weight and parameters of intestinal adaptation (overall bowel and mucosal weight, mucosal DNA and protein, villus height, and crypt depth) were determined at time of killing. By day 3, SBS-HFD rats demonstrated higher duodenal and jejunal bowel and mucosal weights and ileal villus height and jejunal crypt depth vs SBS-NC rats. By day 14 SBS-HFD rats continued to demonstrate increased duodenal and jejunal bowel weight and duodenal mucosal weight vs SBS-NC animals. We conclude that early exposure to HFD both augmented and accelerated structural bowel adaptation in a rat model of SBS.
authors.affiliation: Section of Pediatric Surgery, C.S. Mott Children's Hospital and University of Michigan Medical School, Ann Arbor, MI, USA. igor-dr@internet-zahav.net
authors.forename: Igor
authors.initials: I
authors.lastname: Sukhotnik
authors.forename: Nirit
authors.initials: N
authors.lastname: Mor-Vaknin
authors.forename: Robert A
authors.initials: RA
authors.lastname: Drongowski
authors.forename: Ines
authors.initials: I
authors.lastname: Miselevich
authors.forename: Arnold G
authors.initials: AG
authors.lastname: Coran
authors.forename: Carroll M
authors.initials: CM
authors.lastname: Harmon
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D001835:Body Weight; D004041:Dietary Fats / Q000008:administration & dosage*; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D007421:Intestine, Small / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012778:Short Bowel Syndrome / Q000178:diet therapy / Q000473:pathology / Q000503:physiopathology*; D013997:Time Factors
chemical_list: D004041:Dietary Fats