Individual variation in plasma estradiol-17beta and androgen levels during egg formation in the European starling Sturnus vulgaris: implications for regulation of yolk steroids.
While it is clear that maternal transfer of steroids to egg yolk can have significant effects on offspring phenotype, an unresolved question is whether females can facultatively adjust yolk hormone levels independently of their own plasma levels or whether yolk steroid levels are simply a direct consequence of temporal variation in the female's hormonal status. In part, this is because we lack detailed information about the day-to-day pattern of changes in plasma hormone levels during the laying cycle for non-domesticated birds. Here, we describe changes in plasma estradiol-17beta (E2) and androgens, throughout laying in relation to specific stages of ovarian follicular development in the European starling (Sturnus vulgaris). Plasma E2 levels increased rapidly from the onset of rapid yolk development (RYD) to reach maximum levels in birds with a complete follicle hierarchy (> or = 4 yolky follicles). However, levels decreased linearly throughout the later stages of follicle development returning to pre-breeding values before the final yolky follicle was ovulated. In females with > or = 4 yolky follicles there was 10-fold variation in plasma E2 levels among individual females, but this was not related to plasma levels of the main yolk precursor vitellogenin or to the total mass of yolky follicles developing at the time of blood sampling. In contrast to E2, plasma androgen levels showed only a very gradual linear decline throughout the laying cycle from pre-RYD to clutch completion. Furthermore, androgen levels showed less individual variability: 4-fold variation among females with > or = 4 yolky follicles, although this was also independent of our measures of reproductive function. Data on inter- and intra-individual variation in female hormone levels are important to set-up a priori predictions for, and interpretation of, studies of yolk hormone levels.
Williams
T D
TD
Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC, Canada. tdwillia@sfu.ca
Kitaysky
A S
AS
Vézina
F
F
D000728:Androgens / Q000097:blood*; D000818:Animals; D004530:Egg Yolk / Q000378:metabolism*; D004958:Estradiol / Q000097:blood*; D005260:Female; D008297:Male; D006080:Ovarian Follicle / Q000254:growth & development / Q000378:metabolism; D010058:Oviposition / Q000502:physiology*; D010063:Ovum / Q000378:metabolism*; D011863:Radioimmunoassay / Q000662:veterinary; D012098:Reproduction / Q000502:physiology; D020308:Songbirds / Q000378:metabolism / Q000502:physiology*; D014819:Vitellogenins / Q000097:blood
D000728:Androgens; D014819:Vitellogenins; D004958:Estradiol