title: Ebp2p, the yeast homolog of Epstein-Barr virus nuclear antigen 1-binding protein 2, interacts with factors of both the 60 S and the 40 s ribosomal subunit assembly.
abstract: Ebp2p, the yeast homolog of human Epstein-Barr virus nuclear antigen 1-binding protein 2, is essential for biogenesis of the 60 S ribosomal subunit. Two-hybrid screening exhibited that, in addition to factors necessary for assembly of the 60 S subunit, Ebp2p interacts with Rps16p, ribosomal protein S16, and the 40 S ribosomal subunit assembly factor, Utp11p, as well as Yil019w, the function of which was previously uncharacterized. Depletion of Yil019w resulted in reduction in levels of both of 18 S rRNA and 40 S ribosomal subunit without affecting levels of 25 S rRNA and 60 S ribosomal subunits. 35 S pre-rRNA and aberrant 23 S RNA accumulated, indicating that pre-rRNA processing at sites A(0)-A(2) is inhibited when Yil019w is depleted. Each combination from Yil019w, Utp11p, and Rps16p showed two-hybrid interaction.
authors.affiliation: Department of Bioresource Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Kagamiyama 1-4-4, Higashi-Hiroshima 739-8528, Japan.
authors.forename: Chiharu
authors.initials: C
authors.lastname: Shirai
authors.forename: Tomoko
authors.initials: T
authors.lastname: Takai
authors.forename: Masanobu
authors.initials: M
authors.lastname: Nariai
authors.forename: Chihiro
authors.initials: C
authors.lastname: Horigome
authors.forename: Keiko
authors.initials: K
authors.lastname: Mizuta
mesh_terms: D002352:Carrier Proteins / Q000502:physiology*; D002455:Cell Division; D012322:RNA Precursors / Q000378:metabolism; D012337:RNA, Ribosomal, 18S / Q000378:metabolism; D012270:Ribosomes / Q000737:chemistry / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*; D020798:Two-Hybrid System Techniques
chemical_list: D002352:Carrier Proteins; C499008:EBP2 protein, S cerevisiae; D012322:RNA Precursors; D012337:RNA, Ribosomal, 18S; D029701:Saccharomyces cerevisiae Proteins