title: Frequency and quantity of the parvovirus B19 genome in endomyocardial biopsies from patients with suspected myocarditis or idiopathic left ventricular dysfunction.
abstract: Parvovirus B19 (PB19) has been identified as a possible cause of myocarditis and heart failure in both children and adult patients. This study used real time PCR analysis, to determine the frequency and to quantify PB19 viral genomes in endomyocardial tissue samples from 80 adult patients with clinically suspected myocarditis or idiopathic left ventricular dysfunction and from 36 controls. Histological (Dallas classification) and immunohistological analyses were performed to detect myocardial inflammation in the endomyocardial biopsies.PB19 genomic DNA was found in nine of 80 patients (11.2%), 4 out of 31 (12.9%) patients with inflammatory infiltrates detected via immunohistological methods and 5 out of 49 (10.2%) patients with left ventricular dysfunction without myocardial inflammation. The copy numbers for PB19 DNA ranged between 30 and 3900 per microg of cellular DNA. Four patients with clinically suspected myocarditis had copy numbers for PB19 DNA of 70, 740, 3400 and 3900, respectively, per microg of cellular DNA in the endomyocardial biopsy. Five patients with idiopathic left ventricular dysfunction had copy numbers for PB19 DNA of 30, 38, 52, 58 and 90, respectively, per microg of cellular DNA in the endomyocardial biopsy. The amplicon of one of the nine positive PCR fragment was sequenced and was found to be fully identical in the highly conserved sequence of published Parvovirus B19 VP1/VP2 genes (NCBI gene bank). In all patients, acute myocarditis was excluded according to the Dallas classification. All biopsies of 36 controls with no history of myocarditis or recent viral infection were negative for myocardial inflammation and parvovirus B19 genomes. In summary, Parvovirus B19 DNA is present within the myocardium of patients with suspected myocarditis and idiopathic left ventricular dysfunction and can be detected and quantified in endomyocardial specimens via real time PCR.
authors.affiliation: Department of Cardiology, HELIOS Klinikum Wuppertal, Arrenbergerstr. 20, 42117 Wuppertal, Germany. rklein@klinikum-wuppertal.de
authors.forename: R M
authors.initials: RM
authors.lastname: Klein
authors.forename: H
authors.initials: H
authors.lastname: Jiang
authors.forename: D
authors.initials: D
authors.lastname: Niederacher
authors.forename: O
authors.initials: O
authors.lastname: Adams
authors.forename: M
authors.initials: M
authors.lastname: Du
authors.forename: M
authors.initials: M
authors.lastname: Horlitz
authors.forename: P
authors.initials: P
authors.lastname: Schley
authors.forename: R
authors.initials: R
authors.lastname: Marx
authors.forename: M R
authors.initials: MR
authors.lastname: Lankisch
authors.forename: M U
authors.initials: MU
authors.lastname: Brehm
authors.forename: B E
authors.initials: BE
authors.lastname: Strauer
authors.forename: H E
authors.initials: HE
authors.lastname: Gabbert
authors.forename: T
authors.initials: T
authors.lastname: Scheffold
authors.forename: H
authors.initials: H
authors.lastname: Gülker
mesh_terms: D000328:Adult; D000368:Aged; D015897:Comorbidity; D004699:Endocardium / Q000473:pathology / Q000821:virology; D005260:Female; D016679:Genome, Viral; D005858:Germany / Q000453:epidemiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009205:Myocarditis / Q000453:epidemiology* / Q000473:pathology / Q000821:virology*; D010322:Parvoviridae Infections / Q000453:epidemiology* / Q000235:genetics*; D016732:Parvovirus B19, Human / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D018570:Risk Assessment / Q000379:methods; D012307:Risk Factors; D018487:Ventricular Dysfunction, Left / Q000196:embryology* / Q000473:pathology / Q000821:virology*