[Production and examination of double antigen specific immunoserum for immunochemical detection of occult blood].
OBJECTIVE
The authors have developed an immunochemical procedure and an immunisation method for the simultaneous detection of fecal hemoglobin and albumin to increase the screening effectiveness of colorectal cancers.


METHODS
In the human specific blood testing, bispecific immunoserum recognising two antigens have been produced by glutardyaldehyde-hemoglobin-albumin makromolecule immunisation of goats. The purified and concentrated antiserum with double antibody specifity has been checked in a screening group of 1196 individuals aged over 40 years with Fecatest reservoirs.


CONCLUSIONS
The analytical sensitivity was proved 0.5 microg/ml for both proteins, which was greatly favourable for the screening. Furthermore, the intensity of the immunochemical reactions has grown, and it has increased the safety of the detection without decreasing the specificity. Because the number of the immunochemical tests that could be completed at the same time has been doubled (without excess of cost), this method has increased the effectiveness of the screening with taking care of expense.
Sz  Németh
Mária
M
Klinikai Laboratóriumi Osztály, Országos Onkológiai Intézet, Budapest 1122, Hungary.
Ottó
Szabolcs
S
D000818:Animals; D005243:Feces / Q000737:chemistry*; D006471:Gastrointestinal Hemorrhage / Q000378:metabolism; D006454:Hemoglobins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D009780:Occult Blood
D006454:Hemoglobins