title: Three-dimensional expression of adhesion molecules on the superficial synovial intima of LPS-induced arthritis of the mouse knee analyzed by immuno-SEM.
abstract: Three-dimensional microlocalization of adhesion molecules, i.e. ICAM-1 (intercellular adhesion molecule), VCAM-1 (vascular adhesion molecule), LFA-1 (lymphocyte function-associated antigen), Mac-1 (macrophage differentiation antigen) and VLA-4 (very late activation antigen), expressed on type-A synoviocyte (macrophage-like cell) and type-B synoviocyte (fibroblast-like cell), were detected by immuno-scanning electron microscopy (SEM) to investigate the immunoreactive microenvironment of the superficial synovial intima in lipopolysaccharide (LPS)-induced arthritis of the mouse knee. Type-B synoviocytes extended rich slender processes from the periphery and constructed a cytoplasmic network, to which ICAM-1 was restricted. VCAM-1 was expressed only in the LPS-stimulated group and was relatively limited to the microvilli of type-B synoviocytes. Type-A synoviocytes were located randomly among the network with a smoother surface and expressed Mac-1 and LFA-1, which were counter-receptors for ICAM-1, and VLA-4 for VCAM-1 on the microvilli or lamellipodia. Three-dimensional microlocalization of adhesion molecules suggests that the network constructed by cytoplasmic processes and microvilli of type-B synoviocytes forms the pathway for the migration or the foothold for the fixation of type-A synoviocytes and takes part in forming an immunoreactive environment in the articular cavity.
authors.affiliation: Department of Anatomy and Organ Technology, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.forename: Shinji
authors.initials: S
authors.lastname: Nakamura
authors.forename: Li
authors.initials: L
authors.lastname: Cui
authors.forename: Kohei
authors.initials: K
authors.lastname: Johkura
authors.forename: Naoko
authors.initials: N
authors.lastname: Ogiwara
authors.forename: Katsunori
authors.initials: K
authors.lastname: Sasaki
mesh_terms: D000818:Animals; D001168:Arthritis / Q000139:chemically induced / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002450:Cell Communication / Q000502:physiology; D005121:Extremities / Q000473:pathology; D007596:Joints / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D016253:Microscopy, Immunoelectron; D013583:Synovial Membrane / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure*
chemical_list: D015815:Cell Adhesion Molecules; D008070:Lipopolysaccharides