Skeletal muscle mitochondrial protein metabolism and function in ageing and type 2 diabetes.
OBJECTIVE
Mitochondria are the site of oxidative substrate utilization to produce adenosine triphosphate for normal tissue function. Tissue substrate utilization is impaired in ageing and type 2 diabetes. Defects in mitochondrial gene expression, protein synthesis and function occur with ageing in various tissues including skeletal muscle, and are emerging in individuals with type 2 diabetes. The current review will discuss advances in the understanding of skeletal muscle mitochondrial alterations associated with age and type 2 diabetes.


RESULTS
Insulin acutely stimulates skeletal muscle mitochondrial protein synthesis and adenosine triphosphate production. These insulin effects are impaired in insulin-resistant patients with type 2 diabetes who also exhibit defective basal muscle mitochondrial function. The age-related reduction in mitochondrial adenosine triphosphate production has been confirmed in vivo in skeletal muscle in humans and rodents.


CONCLUSIONS
The emerging concept that insulin stimulates mitochondrial protein synthesis and function indicates potential novel molecular mechanisms of metabolic defects in type 2 diabetes, particularly in the post-prandial period characterized by acute increments of plasma insulin concentrations. The potential relationship between insulin resistance and basal post-absorptive muscle mitochondrial defects should be further investigated. As ageing is characterized by insulin resistance, the hypothesis that impaired insulin action could contribute to age-related muscle mitochondrial dysfunction, and metabolic alterations should be addressed.
Barazzoni
Rocco
R
Department of Clinical Morphological and Technological Sciences, Institute of Clinical Medicine, University of Trieste, Trieste, Italy. barazzon@units.it
D000255:Adenosine Triphosphate / Q000378:metabolism; D000368:Aged; D000375:Aging / Q000378:metabolism*; D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D005260:Female; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D008931:Mitochondria, Muscle / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000187:drug effects / Q000378:metabolism*; D051381:Rats
D007328:Insulin; D011506:Proteins; D000255:Adenosine Triphosphate