Effects of antimicrobial peptides derived from the beetle Allomyrina dichotoma defensin on mouse peritoneal macrophages stimulated with lipopolysaccharide.
We previously reported that synthetic peptides, RLYLRIGRR-NH2 (peptide A) and RLRLRIGRR-NH2 (peptide B), derived from the beetle Allomyrina dichotoma defensin, showed antimicrobial activity against both Gram-positive and negative bacteria and suppressed lipopolysaccharide (LPS)-induced tumor necrosis factor-alpha (TNF-alpha) mRNA expression in a murine macrophage cell line. In this study, inhibitory effects of these peptides in LPS-induced mouse peritoneal macrophage activation were investigated. The supplement of peptide A to macrophages cultured with LPS resulted in a significant decrease in nitric oxide and TNF-alpha production. Furthermore, NF-kappaB activation was also blocked by addition of peptide A. These results indicated that peptide A blocked macrophage activation induced by LPS.
Motobu
Maki
M
Department of Production Diseases, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
Amer
Said
S
Yamada
Manabu
M
Nakamura
Kikuyasu
K
Saido-Sakanaka
Hisako
H
Asaoka
Ai
A
Yamakawa
Minoru
M
Hirota
Yoshikazu
Y
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001517:Coleoptera / Q000737:chemistry*; D023082:Defensins / Q000494:pharmacology*; D008070:Lipopolysaccharides; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000900:Anti-Bacterial Agents; D023082:Defensins; D008070:Lipopolysaccharides; D010455:Peptides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide