Comparative mapping of QTLs for Al tolerance in rice and identification of positional Al-induced genes.
Aluminum (Al) toxicity is the major factor limiting crop productivity in acid soils. In this study, a recombinant inbreed line (RIL) population derived from a cross between an Al sensitive lowland indica rice variety IR1552 and an Al tolerant upland japonica rice variety Azucena, was used for mapping quantitative trait loci (QTLs) for Al tolerance. Three QTLs for relative root length (RRL) were detected on chromosome 1, 9, 12, respectively, and 1 QTL for root length under Al stress is identical on chromosome 1 after one week and two weeks stress. Comparison of QTLs on chromosome 1 from different studies indicated an identical interval between C86 and RZ801 with gene(s) for Al tolerance. This interval provides an important start point for isolating genes responsible for Al tolerance and understanding the genetic nature of Al tolerance in rice. Four Al induced ESTs located in this interval were screened by reverse Northern analysis and confirmed by Northern analysis. They would be candidate genes for the QTL.
Mao
Chuan-zao
CZ
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310029, China.
Yang
Ling
L
Zheng
Bing-song
BS
Wu
Yun-rong
YR
Liu
Fei-yan
FY
Yi
Ke-ke
KK
Wu
Ping
P
D000535:Aluminum / Q000494:pharmacology*; D015152:Blotting, Northern; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant / Q000235:genetics*; D012275:Oryza / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D010641:Phenotype; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D040641:Quantitative Trait Loci / Q000235:genetics*; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000254:growth & development
D000535:Aluminum