[Schistosoma japonicum: screening of cDNA library with sera from self-cured water buffaloes (Bos buffelus)].
OBJECTIVE
To understand and identify the molecules related to the self-cure of Schistosoma japonicum infection in water buffaloes.


METHODS
The S. japonicum adult worm cDNA library was immunologically screened with the sera of self-cured water buffaloes. The positive clones were identified, cloned, sequenced and analysed with software.


RESULTS
Three genes encoding antigens relevant to sera antibodies in water buffaloes were cloned and sequenced. These antigens included paramyosin (Sj97), GST, carbonyl reductase-like 20-beta-hydroxysteroid dehydrogenase(CR/20-beta-HSD).


CONCLUSIONS
Sera from self-cured S. japonicum infected water buffaloes can be used to screen adult worm cDNA library for vaccine development.
Wang
Wen-qin
WQ
Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025.
Liu
Shu-xian
SX
Song
Guang-cheng
GC
Xu
Yu-xin
YX
Cao
Jian-ping
JP
Chen
Jia-xu
JX
D000818:Animals; D002020:Buffaloes / Q000276:immunology*; D018076:DNA, Complementary / Q000276:immunology*; D018509:DNA, Helminth / Q000276:immunology*; D015723:Gene Library; D007106:Immune Sera / Q000276:immunology; D012549:Schistosoma japonicum / Q000235:genetics* / Q000276:immunology; D012554:Schistosomiasis japonica / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology
D018076:DNA, Complementary; D018509:DNA, Helminth; D007106:Immune Sera; D019444:Vaccines, DNA