OTK18 expression in brain mononuclear phagocytes parallels the severity of HIV-1 encephalitis.
OTK18 was isolated by mRNA differential display of human monocyte-derived macrophages (MDM) infected with human immunodeficiency virus type one (HIV-1). Northern blot and real-time reverse transcription polymerase chain reaction showed low levels of OTK18 expression in human tissue, which markedly increased during advanced HIV-1 encephalitis (HIVE). Immunocytochemistry, using rabbit polyclonal antisera, showed OTK18 localized to brain mononuclear phagocytes (MP) in moderate to severe HIV-1 encephalitis. OTK18 expression was selective and not found in HIV-1-infected brain tissue with limited neuropathological abnormalities, nor in cytomegalovirus encephalitis, multiple sclerosis, Alzheimer's disease, or uninfected control brains. Thus, OTK18 expression in brain mononuclear phagocytes is a signature for advanced HIV-1 encephalitis.
Carlson
Kimberly A
KA
Center for Neurovirology and Neurodegenerative Disorders, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-5215, USA.
Limoges
Jenae
J
Pohlman
Garrett D
GD
Poluektova
Larisa Y
LY
Langford
Dianne
D
Masliah
Eliezer
E
Ikezu
Tsuneya
T
Gendelman
Howard E
HE
D015526:AIDS Dementia Complex / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D001921:Brain / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D018450:Disease Progression; D018792:Encephalitis, Viral / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008264:Macrophages / Q000276:immunology / Q000378:metabolism* / Q000821:virology; D009928:Organ Specificity / Q000235:genetics / Q000276:immunology; D012720:Severity of Illness Index; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D014779:Virus Replication / Q000235:genetics / Q000276:immunology
D004268:DNA-Binding Proteins; D014157:Transcription Factors; C104178:ZNF175 protein, human