[Effects of zinc and selenium on the disorders of blood glucose and lipid metabolism and its molecular mechanism in diabetic rats].
OBJECTIVE
In order to study the effects of zinc and selenium on glucose and lipid metabolism and its molecular mechanism in diabetic rats.


METHODS
The Blood glucose, lipid and serum insulin were measured before and after the treatment, and the changes in gene expression among four groups were studied by mRNA differential display technique.


RESULTS
The blood glucose in DM + Zn group, DM + Se group decreased significantly than that before experiment; the levels of serum TG, TC, LDL-c and AI in DM + Zn group and DM + Se group were lower than those of DM group, while the serum HDL-c levels were higher. The serum insulin was also improved obviously. 31 cDNA fragments larger than 400 bp expressed differences in liver between DM + Zn group, DM + Se group and DM group were isolated, 19 of them expressed higher in DM + Zn group and DM + Se group, while the rest expressed higher in DM group.


CONCLUSIONS
It is suggested that zinc and selenium supplementation could partially improve the disorders of glucose and lipid metabolism, and have an impact on some gene expression in diabetic rats, which may contribute to the regulating effects on its disorders of metabolism.
Wu
Yuntang
Y
School of Public Health, Tianjin Medical University, Tianjin 300070, China.
Sun
Zhong
Z
Che
Suping
S
Chang
Hong
H
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood*; D003921:Diabetes Mellitus, Experimental / Q000097:blood*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000494:pharmacology*; D014280:Triglycerides / Q000097:blood; D015032:Zinc / Q000494:pharmacology*
D001786:Blood Glucose; D014280:Triglycerides; D002784:Cholesterol; D012643:Selenium; D015032:Zinc