Targeting of human aFGF gene into silkworm, Bombyx mori L. through homologous recombination.
The long-arm and short-arm genes of fibroin light chain (L-chain) of silkworm, Bombyx Mori L., and the gene of human acidic fibroblast growth factor were cloned respectively and subsequently inserted into a transfer vector pVL1392 used as a tool to target the L-chain region of the silkworm genome. Genomic DNA from their offsprings was extracted and the expected targeting was detected using polymerase chain reaction and DNA sequencing, as well as protein analysis. The results showed that positive events occurred and that the FGF gene was integrated into the L-chain locus through homologous recombination.
Wu
Xiao-feng
XF
Lab of Gene Engineering, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China. wuxiaofeng@zju.edu.cn
Cao
Cui-ping
CP
D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics*; D016220:Fibroblast Growth Factor 1 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005349:Fibroins / Q000235:genetics / Q000378:metabolism; D018390:Gene Targeting; D005822:Genetic Vectors / Q000235:genetics; D016678:Genome; D006801:Humans; D008969:Molecular Sequence Data; D011995:Recombination, Genetic / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D016220:Fibroblast Growth Factor 1; D005349:Fibroins