[Immunoscreening of Schistosoma japonicum adult worm cDNA library with sera vaccinated with cercaria antigen and analysis of novel genes].
OBJECTIVE
To explore antigens possessing common immunogenicity with Schistosoma japonicum (Sj) cercariae antigens, and to find out new candidate antigens for schistosomiasis diagnosis and vaccine.


METHODS
Sj adult cDNA library was screened using sera from rabbits vaccinated with Sj cercariae antigen, the inserts of positive clones were amplified by PCR, all positive clones were sequenced and the data were analysed using Nucleotide BLAST software of NCBI and Expert Protein Analysis System of Swiss Institute of Bioinformatics.


RESULTS
Thirteen positive clones were obtained after three rounds of immunoscreening, and all amplified by PCR. Among four novel genes, SjCAI, SjCA, SjCAI-2 and SjCAI-3 (GenBank accession number: AF495883, AF515834, AY118086 and AY129303, respectively) encoded proteins with 353, 161, 137 and 72 animo acids respectively. Sj CAI protein contained six DNA-binding zinc fingers and showed some homology to gastrula zinc finger protein XLCGF48.2; proteins encoded by SjCA, SjCAI-2 and SjCAI-3 respectively contained N-glycosylation sites and phosphorylation sites.


CONCLUSIONS
Novel genes were obtained by immunoscreening Sj adult cDNA library.
Yuan
Shi-shan
SS
Laboratory of Schistosomiasis, Xiangya School of Medicine, Central South University, Changsha 410078.
Yi
Xin-yuan
XY
Zeng
Xian-fang
XF
Ouyang
Li
L
Wang
Min
M
Tang
Lian-fei
LF
McReynolds
Larry
L
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D017238:Genes, Helminth; D007106:Immune Sera / Q000276:immunology*; D011817:Rabbits; D012549:Schistosoma japonicum / Q000235:genetics* / Q000276:immunology
D000947:Antigens, Helminth; D018076:DNA, Complementary; D007106:Immune Sera