title: Overexpression, purification and characterization of recombinant salmon calcitonin, a therapeutic protein, in Streptomyces avermitilis.
abstract: Calcitonin (CT) is a peptide hormone produced by the parafollicular cells of the thyroid gland in mammals and by the ultimobranchial gland of birds and fish. Salmon calcitonin (sCT), which is more potent and longer lasting than human CT, has been used widely for the treatment of osteoporosis, paget's disease, hypercalcemic shock and chronic pain in terminal cancer patients. sCT is one of the many bioactive peptides that require C-terminal amidation for full biological activity. In this study we describe the over-expression and over-production of C-terminal amidated sCT in recombinant Streptomyces avermitilis. With this approach the utilization of expensive peptide synthesis can be circumvented.
authors.affiliation: Biotechnology Department, Glenmark Laboratories, C-33 Nizamuddin East, New Delhi-110013, India. drchiranjib@yahoo.com
authors.forename: Chiranjib
authors.initials: C
authors.lastname: Chakraborty
authors.forename: Shyam
authors.initials: S
authors.lastname: Nandi
authors.forename: S
authors.initials: S
authors.lastname: Sinha
mesh_terms: D000595:Amino Acid Sequence; D000700:Analgesics / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D002116:Calcitonin / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D010146:Pain / Q000188:drug therapy; D010957:Plasmids / Q000235:genetics; D011499:Protein Processing, Post-Translational / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000627:therapeutic use; D016415:Sequence Alignment; D013302:Streptomyces; D013961:Thyroid Gland / Q000378:metabolism
chemical_list: D000700:Analgesics; D018076:DNA, Complementary; D011994:Recombinant Proteins; C028815:salmon calcitonin; D002116:Calcitonin