title: Cadherin-2 function in the cranial ganglia and lateral line system of developing zebrafish.
abstract: Cadherins are cell surface molecules that mediate cell-cell adhesion through homophilic interactions. Cadherin-2 (also called N-cadherin), a member of classic cadherin subfamily, has been shown to play important roles in development of a variety of tissues and organs, including the nervous system. We recently reported that cadherin-2 was strongly expressed by the majority of cranial ganglia and lateral line system of developing zebrafish. To gain insight into cadherin-2 role in the formation of these structures, we have used several markers to analyze zebrafish embryos injected with a specific cadherin-2 antisense morpholino oligonucleotide (cdh2MO). We find that development of several cranial ganglia, including the trigeminal, facial, and vagal ganglia, and the lateral line ganglia and neuromasts of the cdh2MO-injected embryos are severely disrupted. These phenotypes were confirmed by analyzing a cadherin-2 mutant, glass onion. Our results suggest that cadherin-2 function is crucial for the normal formation of the zebrafish lateral line system and a subset of cranial ganglia.
authors.affiliation: Department of Biology, University of Akron, Akron, Ohio 44325-3908, USA.
authors.forename: A E
authors.initials: AE
authors.lastname: Kerstetter
authors.forename: E
authors.initials: E
authors.lastname: Azodi
authors.forename: J A
authors.initials: JA
authors.lastname: Marrs
authors.forename: Q
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000818:Animals; D001479:Basal Ganglia / Q000196:embryology; D001921:Brain / Q000196:embryology; D015820:Cadherins / Q000096:biosynthesis; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002462:Cell Membrane / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008953:Models, Anatomic; D009474:Neurons / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000737:chemistry
chemical_list: D015820:Cadherins; C489083:Cdh2 protein, mouse; D015815:Cell Adhesion Molecules; D016376:Oligonucleotides, Antisense; D029961:Zebrafish Proteins; C502406:fat1 protein, mouse