Isolation from Eucalyptus occidentalis and identification of a new kaempferol derivative that induces apoptosis in human myeloid leukemia cells.
In this paper we report the isolation and structural elucidation of a new flavonoid (1) and three known compounds, 6,8-di-C-methylkaempferol 3-methyl ether (2), oleanolic acid, and 2 alpha,3 beta-dihydroxyurs-12-en-28-oic acid, from aerial parts of Eucalyptus occidentalis collected in Algeria. Flavonoids 1 and 2 were used to study their biological activities on the human promyelocytic leukemia cell line, HL-60. Our data show that these compounds induce morphological changes and internucleosomal DNA fragmentation characteristic of apoptotic cell death, which is mediated by caspase-8/caspase-3 activation and cytochrome c release.
Benyahia
Samira
S
Laboratoire de Valorisation des Ressources Naturelles, Université Mentouri, Constantine, Route de Aín El Bey 25000 Constantine, Algeria.
Benayache
Samir
S
Benayache
Fadila
F
Quintana
José
J
López
Matías
M
León
Francisco
F
Hernández
Juan C
JC
Estévez
Francisco
F
Bermejo
Jaime
J
D000462:Algeria; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D053181:Caspase 8; D020169:Caspases / Q000378:metabolism; D003574:Cytochrome c Group / Q000378:metabolism; D053938:DNA Fragmentation; D004789:Enzyme Activation; D005052:Eucalyptus; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018922:HL-60 Cells / Q000737:chemistry*; D006801:Humans; D044949:Kaempferols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D007951:Leukemia, Myeloid; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry
C485060:6,8-di-C-methylkaempferol 3-methyl ether; D000970:Antineoplastic Agents; D003574:Cytochrome c Group; D005419:Flavonoids; D044949:Kaempferols; C076615:cytochrome c''; D009828:Oleanolic Acid; C006552:kaempferol; C505407:CASP3 protein, human; C505530:CASP8 protein, human; D053148:Caspase 3; D053181:Caspase 8; D020169:Caspases