title: Antemortem diagnosis of canine neural angiostrongylosis using ELISA.
abstract: A 5-month-old female Kelpie developed paraparesis, hind limb ataxia and spinal hyperaesthesia 4 days after ovariohysterectomy. Neurological examination demonstrated upper motor neuron signs in the pelvic limbs with lower motor neuron signs in the tail. Cerebrospinal fluid analysis demonstrated an increased protein concentration and marked eosinophilic pleocytosis. The dog was known to have eaten rats, snails and slugs. A tentative diagnosis of neural angiostrongylosis was made and later confirmed using an ELISA based on soluble antigens obtained from larval 4 Angiostrongylus cantonensis. Antibody titres from the patient's serum and CSF were 800 and 6400, respectively. The dog was treated successfully with prednisolone. ELISA testing of serum may provide a non-invasive means for diagnosing neural angiostrongylosis in dogs.
authors.affiliation: University Veterinary Centre Sydney, Faculty of Veterinary Science, The University of Sydney, New South Wales 2006.
authors.forename: J
authors.initials: J
authors.lastname: Lunn
authors.forename: R
authors.initials: R
authors.lastname: Lee
authors.forename: P
authors.initials: P
authors.lastname: Martin
authors.forename: R
authors.initials: R
authors.lastname: Malik
mesh_terms: D017185:Angiostrongylus cantonensis / Q000276:immunology*; D000818:Animals; D000871:Anthelmintics / Q000627:therapeutic use; D000909:Antibodies, Helminth / Q000097:blood* / Q000134:cerebrospinal fluid; D000947:Antigens, Helminth / Q000032:analysis; D020809:Central Nervous System Helminthiasis / Q000175:diagnosis / Q000188:drug therapy / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis* / Q000188:drug therapy / Q000469:parasitology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D011239:Prednisolone / Q000627:therapeutic use; D017206:Strongylida Infections / Q000175:diagnosis / Q000188:drug therapy / Q000662:veterinary*; D016896:Treatment Outcome
chemical_list: D000871:Anthelmintics; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D011239:Prednisolone