title: Increased frequency of the k-ras G12C mutation in MYH polyposis colorectal adenomas.
abstract: Colorectal tumours from MYH polyposis patients display an excess of somatic G : C --> T : A transversions in the adenomatous polyposis coli gene. Here, we identify k-ras mutations in nine out of 54 (16.7%) MYH polyposis tumours. Their presence was associated with increased dysplasia and tubulovillous morphology (P=0.005). G : C --> T : A transversions in k-ras were significantly more frequent in MYH polyposis adenomas than in sporadic or familial adenomatous polyposis-associated tumours (P<or=0.002), and all resulted in a glycine-to-cysteine substitution at codon 12.
authors.affiliation: Institute of Medical Genetics, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK.
authors.forename: S
authors.initials: S
authors.lastname: Jones
authors.forename: S
authors.initials: S
authors.lastname: Lambert
authors.forename: G T
authors.initials: GT
authors.lastname: Williams
authors.forename: J M
authors.initials: JM
authors.lastname: Best
authors.forename: J R
authors.initials: JR
authors.lastname: Sampson
authors.forename: J P
authors.initials: JP
authors.lastname: Cheadle
mesh_terms: D000236:Adenoma / Q000235:genetics* / Q000473:pathology; D011125:Adenomatous Polyposis Coli / Q000235:genetics* / Q000473:pathology; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D004252:DNA Mutational Analysis; D011905:Genes, ras; D018095:Germ-Line Mutation; D006801:Humans; D016133:Polymerase Chain Reaction