Identification of 36 kDa phosphoprotein in fibrous sheath of hamster spermatozoa.
In our previous studies (Fujinoki et al., 2001, 2003), we reported that two types of 36 kDa proteins, designated 36K-A protein and 36K-B protein, obtained from hamster sperm flagella, are associated with motility activation and phosphorylated in a cAMP-dependent manner at serine residues. In the present experiments, we focused on the hamster (Mesocricetus auratus) 36K-A protein, which was analyzed by peptide mass finger printing and amino acid sequencing. The results suggest that 36K-A protein is a pyruvate dehydrogenase E1 component beta subunit lacking the N-terminal 30 amino acids. Moreover, our results suggest that 36 K-A protein is localized in the fibrous sheath of the principal piece of hamster spermatazoa.
Fujinoki
Masakatsu
M
Department of Physiology, Dokkyo University School of Medicine, 880 kitakobayasi, Mibu, Tochigi 321-0293, Japan. fujinoki@dokkyomed.ac.jp
Kawamura
Takeshi
T
Toda
Toshifusa
T
Ohtake
Hideki
H
Ishimoda-Takagi
Tadashi
T
Shimizu
Nobuyoshi
N
Yamaoka
Sadao
S
Okuno
Makoto
M
D000595:Amino Acid Sequence; D000818:Animals; D006224:Cricetinae; D000242:Cyclic AMP / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis / Q000737:chemistry; D010750:Phosphoproteins / Q000737:chemistry / Q000302:isolation & purification*; D010766:Phosphorylation; D021122:Protein Subunits / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D030481:Pyruvate Dehydrogenase (Lipoamide) / Q000378:metabolism; D012694:Serine / Q000737:chemistry; D013082:Sperm Tail / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism; D013094:Spermatozoa / Q000737:chemistry*
D010455:Peptides; D010750:Phosphoproteins; D021122:Protein Subunits; D012694:Serine; D000242:Cyclic AMP; D030481:Pyruvate Dehydrogenase (Lipoamide); C111305:pyruvate dehydrogenase E1 beta subunit