title: Genomic DNA-chip hybridization in t(11;14)-positive mantle cell lymphomas shows a high frequency of aberrations and allows a refined characterization of consensus regions.
abstract: Tumor samples of 53 patients with t(11;14)-positive mantle cell lymphomas (MCLs) were analyzed by matrix-based comparative genomic hybridization (matrix-CGH) using a dedicated DNA array. In 49 cases, genomic aberrations were identified. In comparison to chromosomal CGH, a 50% higher number of aberrations was found and the high specificity of matrix-CGH was demonstrated by fluorescence in situ hybridization (FISH) analyses. The 11q gains and 13q34 deletions, which have not been described as frequent genomic aberrations in MCL, were identified by matrix-CGH in 15 and 26 cases, respectively. For several genomic aberrations, novel consensus regions were defined: 8p21 (size of the consensus region, 2.4 megabase pairs [Mbp]; candidate genes: TNFRSF10B, TNFRSF10C, TNFRSF10D); 10p13 (2.7 Mbp; BMI1); 11q13 (1.4 Mbp; RELA); 11q13 (5.2 Mbp; CCND1); 13q14 (0.4 Mbp; RFP2, BCMSUN) and 13q34 (6.9 Mbp). In univariate analyses correlating genomic aberrations and clinical course, 8p- and 13q14- deletions were associated with an inferior overall survival. These data provide a basis for further studies focusing on the identification of pathogenetically or clinically relevant genes in MCL.
authors.affiliation: Department of Innere Medizin I, Medizinische Klinik der Universität Ulm, Ulm, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Kohlhammer
authors.forename: Carsten
authors.initials: C
authors.lastname: Schwaenen
authors.forename: Swen
authors.initials: S
authors.lastname: Wessendorf
authors.forename: Karlheinz
authors.initials: K
authors.lastname: Holzmann
authors.forename: Hans A
authors.initials: HA
authors.lastname: Kestler
authors.forename: Dirk
authors.initials: D
authors.lastname: Kienle
authors.forename: Thomas F E
authors.initials: TF
authors.lastname: Barth
authors.forename: Peter
authors.initials: P
authors.lastname: Möller
authors.forename: German
authors.initials: G
authors.lastname: Ott
authors.forename: Jörg
authors.initials: J
authors.lastname: Kalla
authors.forename: Bernhard
authors.initials: B
authors.lastname: Radlwimmer
authors.forename: Armin
authors.initials: A
authors.lastname: Pscherer
authors.forename: Stephan
authors.initials: S
authors.lastname: Stilgenbauer
authors.forename: Hartmut
authors.initials: H
authors.lastname: Döhner
authors.forename: Peter
authors.initials: P
authors.lastname: Lichter
authors.forename: Martin
authors.initials: M
authors.lastname: Bentz
mesh_terms: D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D016384:Consensus Sequence; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D020522:Lymphoma, Mantle-Cell / Q000235:genetics* / Q000473:pathology; D009367:Neoplasm Staging; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012189:Retrospective Studies; D014178:Translocation, Genetic