title: Rapid enzymatic transglycosylation and oligosaccharide synthesis in a microchip reactor.
abstract: Glycosidase-promoted hydrolysis was performed in a microreaction channel. The result was compared with the reaction in a micro-test tube. Transgalactosylation on p-nitrophenyl-2-acetamide-2-deoxy-beta-D-glucopyranoside was also examined in a microreaction channel, because transglycosylation is a useful method for oligosaccharide synthesis. We examined both the forward reaction, i.e., hydrolysis, and the reverse reaction, i.e., transglycosylation, in the microreaction channel. The results showed that both hydrolysis and transglycosylation were enhanced in the microreaction channel compared with the batch reaction.
authors.affiliation: Department of Bio-Environmental Chemistry, Kyushu School of Engineering, Kinki University, Kayanomori 11-6, Iizuka-shi, Fukuoka, 820-8555, Japan.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Kanno
authors.forename: Hideaki
authors.initials: H
authors.lastname: Maeda
authors.forename: Showta
authors.initials: S
authors.lastname: Izumo
authors.forename: Masakazu
authors.initials: M
authors.lastname: Ikuno
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Takeshita
authors.forename: Asuka
authors.initials: A
authors.lastname: Tashiro
authors.forename: Masayuki
authors.initials: M
authors.lastname: Fujii
mesh_terms: D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000737:chemistry; D006031:Glycosylation; D008904:Miniaturization; D009844:Oligosaccharides / Q000138:chemical synthesis*; D001616:beta-Galactosidase / Q000737:chemistry*
chemical_list: D009844:Oligosaccharides; C014124:4-nitrophenyl-N-acetyl-2-deoxyglucopyranoside; D001616:beta-Galactosidase; D000117:Acetylglucosamine