PDMS-glass hybrid microreactor array with embedded temperature control device. Application to cell-free protein synthesis.
A microreactor array was developed which enables high-throughput cell-free protein synthesis. The microreactor array is composed of a temperature control chip and a reaction chamber chip. The temperature control chip is a glass-made chip on which temperature control devices, heaters and temperature sensors, are fabricated with an ITO (indium tin oxide) resistive material. The reaction chamber chip is fabricated by micromolding of PDMS (polydimethylsiloxane), and is designed to have an array of reaction chambers and flow channels for liquid introduction. The microreactor array is assembled by placing the reaction chamber chip on the temperature control chip. The small thermal mass of the reaction chamber resulted in a short thermal time constant of 170 ms for heating and 3 s for cooling. The performance of the microreactor array was examined through the experiments of cell-free protein synthesis. By measuring the fluorescence emission from the products, it was confirmed that GFP (Green Fluorescent Protein) and BFP (Blue Fluorescent Protein) were successfully synthesized using Escherichia coli extract.
Yamamoto
Takatoki
T
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan. takatoki@iis.u-tokyo.ac.jp
Fujii
Takahiko
T
Nojima
Teruo
T
D000327:Adsorption; D002474:Cell-Free System; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000138:chemical synthesis*; D009757:Nylons / Q000737:chemistry*; D013696:Temperature
D004129:Dimethylpolysiloxanes; D008164:Luminescent Proteins; D009757:Nylons; C409504:blue fluorescent protein, Aequorea victoria; C085707:poly(dimethylsiloxane)-polyamide copolymer; D049452:Green Fluorescent Proteins