Golgi targeting of ARF-like GTPase Arl3p requires its Nalpha-acetylation and the integral membrane protein Sys1p.
Myristoylation of ARF family GTPases is required for their association with Golgi and endosomal membranes, where they regulate protein sorting and the lipid composition of these organelles. The Golgi-localized ARF-like GTPase Arl3p/ARP lacks a myristoylation signal, indicating that its targeting mechanism is distinct from myristoylated ARFs. We demonstrate that acetylation of the N-terminal methionine of Arl3p requires the NatC N(alpha)-acetyltransferase and that this modification is required for its Golgi localization. Chemical crosslinking and fluorescence microscopy experiments demonstrate that localization of Arl3p also requires Sys1p, a Golgi-localized integral membrane protein, which may serve as a receptor for acetylated Arl3p.
Setty
Subba Rao Gangi
SR
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, 421 Curie Blvd. BRB 2/3 room 1010, Philadelphia, PA 19104-6058, USA.
Strochlic
Todd I
TI
Tong
Amy Hin Yan
AH
Boone
Charles
C
Burd
Christopher G
CG
D020727:ADP-Ribosylation Factors / Q000235:genetics / Q000378:metabolism*; D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism*; D001191:Arylamine N-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D063210:N-Terminal Acetyltransferase B; D021381:Protein Transport / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D033921:Vesicular Transport Proteins
C077082:MAK10 protein, S cerevisiae; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; C444081:Sys1 protein, S cerevisiae; D033921:Vesicular Transport Proteins; D000123:Acetyltransferases; D063210:N-Terminal Acetyltransferase B; C077299:MAK3 protein, S cerevisiae; D001191:Arylamine N-Acetyltransferase; D020727:ADP-Ribosylation Factors; C490249:ARL3 protein, S cerevisiae