1,536-well assay development and screening using whole cell displacement binding and laser scanning imaging.
A screen of a GPCR against Pharmacopeia's combinatorial libraries was performed using 1,536-well plates in a 1.5-microl assay volume with an LSI that was specially modified to enable detection at these volumes. The screen encompassed approximately 4 x 10(6) compounds. The assay uses a CHO cell line that expresses human CXCR1. The plate format chosen was the Corning 1536 low-profile wafer plate. The performance of the screen is evaluated, and the necessity to obtain cytotoxicity data from the same well is described.
Auld
Douglas S
DS
Pharmacopeia Inc., Box 5350, Princeton, NJ 08543, USA. dauld@pharmacop.com
Dunn
David A
DA
Lehrach
John M
JM
McCoy
Paul
P
Swanson
Rob
R
D000818:Animals; D001667:Binding, Competitive; D016466:CHO Cells; D020650:Combinatorial Chemistry Techniques; D006224:Cricetinae; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods*; D008904:Miniaturization; D023062:Receptors, Interleukin-8A / Q000378:metabolism*; D015203:Reproducibility of Results
D023062:Receptors, Interleukin-8A