title: Modeling of the pyruvate production with Escherichia coli in a fed-batch bioreactor.
abstract: A family of 10 competing, unstructured models has been developed to model cell growth, substrate consumption, and product formation of the pyruvate producing strain Escherichia coli YYC202 ldhA::Kan strain used in fed-batch processes. The strain is completely blocked in its ability to convert pyruvate into acetyl-CoA or acetate (using glucose as the carbon source) resulting in an acetate auxotrophy during growth in glucose minimal medium. Parameter estimation was carried out using data from fed-batch fermentation performed at constant glucose feed rates of q(VG)=10 mL h(-1). Acetate was fed according to the previously developed feeding strategy. While the model identification was realized by least-square fit, the model discrimination was based on the model selection criterion (MSC). The validation of model parameters was performed applying data from two different fed-batch experiments with glucose feed rate q(VG)=20 and 30 mL h(-1), respectively. Consequently, the most suitable model was identified that reflected the pyruvate and biomass curves adequately by considering a pyruvate inhibited growth (Jerusalimsky approach) and pyruvate inhibited product formation (described by modified Luedeking-Piret/Levenspiel term).
authors.affiliation: Faculty of Chemical Engineering and Technology, University of Zagreb, Marulicev trg 19, 10000 Zagreb, Croatia. bzelic@marie.fkit.hr
authors.forename: B
authors.initials: B
authors.lastname: Zelić
authors.forename: D
authors.initials: D
authors.lastname: Vasić-Racki
authors.forename: C
authors.initials: C
authors.lastname: Wandrey
authors.forename: R
authors.initials: R
authors.lastname: Takors
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D003198:Computer Simulation; D004926:Escherichia coli / Q000502:physiology*; D005947:Glucose / Q000378:metabolism*; D008954:Models, Biological; D019289:Pyruvic Acid / Q000378:metabolism*
chemical_list: D019289:Pyruvic Acid; D005947:Glucose