Murine B-cell leukemia lymphoma (BCL1) cells as a target for NK cell-mediated immunotherapy.
Natural killer (NK) cells are important for their ability to recognize and lyse tumor cells and virus infected cells. NK cells express triggering receptors that are specific for non-MHC ligands. This article describes the 35S release cytotoxic assay, which measures the ability of NK cells derived from spleen cells taken from polyIC-treated mice to lyse B-cell leukemia (BCL1) cells. BCL1 cells express ligands for NKp46 on the cell surface membrane and they are sensitive to allogeneic but not syngeneic IL-2 activated natural killer cells.
Weiss
L
L
Department of Bone Marrow Transplantation and Cancer Immunotherapy, Cell Therapy and Transplantation Immunobiology Research Center, Hadassah University Hospital, Jerusalem, Israel.
Reich
S
S
Mandelboim
O
O
Slavin
S
S
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic; D007167:Immunotherapy / Q000379:methods*; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology*; D015448:Leukemia, B-Cell / Q000473:pathology* / Q000628:therapy; D008024:Ligands; D016393:Lymphoma, B-Cell / Q000473:pathology* / Q000628:therapy; D008562:Membrane Glycoproteins / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D055627:Natural Cytotoxicity Triggering Receptor 1; D011070:Poly I-C / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000032:analysis; D013460:Sulfur Isotopes
D008024:Ligands; D008562:Membrane Glycoproteins; C112260:NCR1 protein, human; D055627:Natural Cytotoxicity Triggering Receptor 1; D011971:Receptors, Immunologic; D013460:Sulfur Isotopes; D011070:Poly I-C