Flow injection chemiluminescent determination of tetracycline using a tris(2,2'-bipyridine)ruthenium(II)-cerium(IV) sulphate system.
A flow-injection chemiluminescence method for the determination of tetracycline was developed. The method is based on an enhancement by tetracycline of the chemiluminescence light emission of tris(2,2'-bipyridine)ruthenium(II). In sulphuric acid medium, the chemiluminescence is generated by the continuous oxidation of tris(2,2'-bipyridine)ruthenium(II) by cerium (IV) sulphate. The light-emission intensity is greatly enhanced in the presence of tetracycline. Under the optimum conditions, the calibration curve is linear over the range 3.75 x 10(-8) g/mL-1.5 x 10(-5) g/mL for tetracycline with the linear equation: deltaINT = 205.898 x C - 20.442 (R2 = 0.9974). The detection limit is 3.27 x 10(-8) g/mL. The proposed method was also successfully used to determine tetracycline in pharmaceutical formulation (mean recovery of tetracycline, 100.7%).
Guo
Liangqia
L
Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, People's Republic of China.
Xie
Zenghong
Z
Lin
Xucong
X
Liu
Xiaohua
X
Zhang
Weilin
W
Chen
Guonan
G
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry*; D002563:Cerium / Q000737:chemistry*; D056831:Coordination Complexes; D017022:Flow Injection Analysis; D007700:Kinetics; D008163:Luminescent Measurements; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013431:Sulfates / Q000737:chemistry*; D013464:Sulfuric Acids / Q000737:chemistry; D013752:Tetracycline / Q000032:analysis*; D017975:Ruthenium Compounds
D015082:2,2'-Dipyridyl; D002563:Cerium; D056831:Coordination Complexes; D009942:Organometallic Compounds; D013431:Sulfates; D013464:Sulfuric Acids; D013752:Tetracycline; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds; C107379:cerium sulfate; C033158:sulfuric acid