title: Circulating auto-antibody against hepatoma-derived growth factor (HDGF) in patients with ulcerative colitis.
abstract: OBJECTIVE
  Various circulating auto-antibodies have been reported in patients with ulcerative colitis. Hepatoma-derived growth factor (HDGF) is a mitogen, localized dominantly in the nucleus of proliferating cells. In this study, we demonstrated the circulating anti-HDGF auto-antibody and investigated its clinical roles in patients with ulcerative colitis.
  
  
  METHODS
  Anti-HDGF IgG antibodies were measured by the enzyme-linked immunosorbent assay with recombinant HDGF in 20 healthy volunteers and 40 patients with ulcerative colitis.
  
  
  RESULTS
  Circulating anti-HDGF antibody was detected in the serum of a patient with total colitis by Western blotting. Anti-HDGF auto-antibodies were detected at 65.6% in the serum of patients with total/left-sided colitis, compared with healthy subjects at 10%. During active stage, the circulating anti-HDGF auto-antibodies were detected at a higher frequency of 78.3% than those in remission stage at 37.5%. Furthermore, the titers during active colitis were higher than those during the remission stage. Anti-HDGF auto-antibodies were not detected in any patients with proctitis.
  
  
  CONCLUSIONS
  These findings suggest that anti-HDGF auto-antibodies in the serum of patients with ulcerative colitis would help to classify the total/left-sided colitis from proctitis, and the serial measurement of the titer would also be a good marker for the active colitis.
authors.affiliation: Department of Molecular Medicine, Osaka University Graduate School of Medicine, Suita, Osaka, Japan. nakamura@hyo-med.ac.jp
authors.forename: Hideji
authors.initials: H
authors.lastname: Nahamura
authors.forename: Kenya
authors.initials: K
authors.lastname: Yoshida
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Kishima
authors.forename: Hirayuki
authors.initials: H
authors.lastname: Enomoto
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Uyama
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Kuroda
authors.forename: Yorihide
authors.initials: Y
authors.lastname: Okuda
authors.forename: Tomonori
authors.initials: T
authors.lastname: Hirotani
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ito
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kawase
mesh_terms: D001323:Autoantibodies / Q000097:blood*; D015153:Blotting, Western; D003093:Colitis, Ulcerative / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000276:immunology* / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D011349:Proctitis / Q000276:immunology
chemical_list: D001323:Autoantibodies; D036341:Intercellular Signaling Peptides and Proteins; C051610:hepatoma-derived growth factor