title: A reverse-phase HPLC assay for the simultaneous determination of enrofloxacin and ciprofloxacin in pig faeces.
abstract: A reverse-phase HPLC assay is described for the simultaneous assay of enrofloxacin (ENR) and ciprofloxacin (CPX) in pig faeces. Extraction used dichloromethane, 2-propanol and 0.3M ortho-phosphoric acid (1:5:4 v/v/v). Separation was achieved using a Spherisorb S5 C8 column, heated to 50 degrees C and a mobile phase of 0.16% ortho-phosphoric acid (adjusted to pH 3.0 with tetrabutylammonium hydroxide solution) with 20 ml acetonitrile per litre solution. The method used fluorescence detection (Ex 310 nm; Em 445 nm), a flow rate of 1 ml/min and a 20 microl injection volume. Retention times were approximately 6 min for ciprofloxacin and 10 min for enrofloxacin. The linearity range for both compounds was 0-20 mg/kg, lowest limit of quantification 0.3 mg/kg and recoveries were >92%.
authors.affiliation: Microbiology Department, Bristol Centre for Antimicrobial Research and Evaluation, Southmead Hostital, North Bristol NHS Trust, Bristol, BS10 5NB, UK. sunderlandj@hotmail.com
authors.forename: Julie
authors.initials: J
authors.lastname: Sunderland
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Lovering
authors.forename: Caroline M
authors.initials: CM
authors.lastname: Tobin
authors.forename: Alasdair P
authors.initials: AP
authors.lastname: MacGowan
authors.forename: John M
authors.initials: JM
authors.lastname: Roe
authors.forename: Anne A
authors.initials: AA
authors.lastname: Delsol
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002939:Ciprofloxacin / Q000032:analysis* / Q000302:isolation & purification; D000077422:Enrofloxacin; D005243:Feces / Q000737:chemistry*; D024841:Fluoroquinolones / Q000032:analysis* / Q000302:isolation & purification; D015363:Quinolones / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine / Q000502:physiology*
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D015363:Quinolones; D000077422:Enrofloxacin; D002939:Ciprofloxacin