Mycoplasma gallisepticum--induced alterations in cytokine genes in chicken cells and embryos.
Mycoplasma gallisepticum (MG) was used to infect the MSB-1 cells, the HD-11 cells, and chicken embryos. The RNA from these cells and the embryonic spleen cells were extracted and assayed for the expression of cytokine genes using reverse transcription-polymerase chain reaction. The results showed that MG infection suppressed the expression of the IL-8 gene in the MSB-1 cells and enhanced the expression of the IL-8 and IL-6 genes in the HD-11 cells; a slightly increased expression of macrophage inflammatory protein-1beta (MIP-1beta) in MG-infected spleen cells was noted. Because of the divergent response in cytokine expression in different cell types, more work needs to be done before a correlation between the cytokine gene expression and the pathogenesis of a disease can be made.
Lam
K M
KM
Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D016207:Cytokines / Q000235:genetics*; D020869:Gene Expression Profiling; D009175:Mycoplasma Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary; D045686:Mycoplasma gallisepticum / Q000472:pathogenicity*; D011201:Poultry Diseases / Q000235:genetics / Q000276:immunology
D016207:Cytokines