Risk assessment of listeriosis linked to the consumption of two soft cheeses made from raw milk: Camembert of Normandy and Brie of Meaux.
This article reports a quantitative risk assessment of human listeriosis linked to the consumption of soft cheeses made from raw milk. Risk assessment was based on data purposefully acquired inclusively over the period 2000-2001 for two French cheeses, namely: Camembert of Normandy and Brie of Meaux. Estimated Listeria monocytogenes concentration in raw milk was on average 0.8 and 0.3 cells/L, respectively, in Normandy and Brie regions. A Monte Carlo simulation was used to account for the time-temperature history of the milk and cheeses from farm to table. It was assumed that cell progeny did not spread within the solid cheese matrix (as they would be free to do in liquid broth). Interaction between pH and temperature was accounted for in the growth model. The simulated proportion of servings with no L. monocytogenes cell was 88% for Brie and 82% for Camembert. The 99th percentile of L. monocytogenes cell numbers in servings of 27 g of cheese was 131 for Brie and 77 for Camembert at the time of consumption, corresponding respectively to three and five cells of L. monocytogenes per gram. The expected number of severe listeriosis cases would be < or =10(-3) and < or =2.5 x 10(-3) per year for 17 million servings of Brie of Meaux and 480 million servings of Camembert of Normandy, respectively.
Sanaa
Moez
M
Epidemiology and Risk Analysis Unit, National Veterinary School of Alfort, Alfort, France. msanaa@vet-alfort.fr
Coroller
Louis
L
Cerf
Olivier
O
D000818:Animals; D002611:Cheese / Q000382:microbiology*; D015169:Colony Count, Microbial; D003198:Computer Simulation; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000453:epidemiology / Q000209:etiology*; D005602:France / Q000453:epidemiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008089:Listeria monocytogenes / Q000254:growth & development / Q000302:isolation & purification; D008088:Listeriosis / Q000453:epidemiology / Q000209:etiology*; D008892:Milk / Q000382:microbiology; D008954:Models, Biological; D009010:Monte Carlo Method; D018570:Risk Assessment