title: Evaluation of adrenal function in serum and feces of Steller sea lions (Eumetopias jubatus): influences of molt, gender, sample storage, and age on glucocorticoid metabolism.
abstract: Fecal corticosterone concentrations, measured via radioimmunoassay (RIA), were validated as a method to monitor adrenal function in Steller sea lion physiology. Quantification of adrenal response to an acute stressor and relevance of data produced by developed methodologies was determined through physiological challenge with exogenous administration of adrenocorticotropic hormone (ACTH) to captive adult, reproductively intact, Steller sea lions of both sexes (n=3, 1 male, 2 female) during seasonal molt. Following ACTH administration, serial blood and fecal samples were collected and analyzed by RIA to determine adrenal response. Storage regimens and weather exposure were examined to establish external impact on fecal corticosterone concentrations. High-pressure liquid chromatography (HPLC) of both serum and feces of Steller sea lions was employed to explore potential gender-based differences extant in either sample media. ACTH challenges produced >3-fold increases in serum cortisol concentrations which were reflected in >18-fold increases in fecal corticosterone concentrations post-injection at 3.25 and 32 h, respectively, and fecal corticosterone concentrations returned to baseline 52 h post-injection. Neither outdoor exposure to weather nor variation in duration and temperature of freezer storage impacted fecal corticosterone concentrations. HPLC of individual fecal samples produced eluate immunoreactivity profiles that differed consistently with both sex and age class. Techniques developed herein effectively detected physiologically relevant corticosterone data in Steller sea lion feces, unaffected by conditions likely to be encountered with field collection samples. Additionally, results quantify an acute response to ACTH and provide methodology for examining chronically heightened adrenal activity in Steller sea lions.
authors.affiliation: University of Alaska, Fairbanks and Alaska SeaLife Center, P.O. Box 1329, 301 Railway Avenue, Seward, AL 99664, USA.
authors.forename: Kendall L
authors.initials: KL
authors.lastname: Mashburn
authors.forename: Shannon
authors.initials: S
authors.lastname: Atkinson
mesh_terms: D000311:Adrenal Glands / Q000502:physiology*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000367:Age Factors; D018685:Anesthetics, Inhalation / Q000494:pharmacology; D000818:Animals; D003345:Corticosterone / Q000097:blood; D005243:Feces / Q000737:chemistry*; D005260:Female; D006854:Hydrocortisone / Q000097:blood; D007530:Isoflurane / Q000494:pharmacology; D008297:Male; D011863:Radioimmunoassay / Q000662:veterinary; D046070:Sea Lions / Q000097:blood / Q000502:physiology*; D012737:Sex Factors; D013048:Specimen Handling / Q000592:standards / Q000662:veterinary
chemical_list: D018685:Anesthetics, Inhalation; D000324:Adrenocorticotropic Hormone; D007530:Isoflurane; D003345:Corticosterone; D006854:Hydrocortisone