title: [Expression and identification of cecropin D gene cloned in Pichia pastoris yeast cells].
abstract: OBJECTIVE
  Cecropin D gene was cloned into methanol nutritional vector-pPICZ alpha-A, linked with alpha-factor signal peptide.
  
  
  METHODS
  Positive recombinant was gotten through PCR and EcoR I restriction enzyme. The positive recombinant vector was transformed into Pichia pastoris yeast strain GS115.
  
  
  RESULTS
  The positive recombinant yeast velum was fermented and its expressive product was assayed by the method of anti-germ.
  
  
  CONCLUSIONS
  Cecropin D gene was cloned into yeast cell and its expressive products has high activity of killing bacteria, Which could be developed into a new type of anti-bacterial medicine.
authors.affiliation: South China University of Technology, Guangzhou 510642, China.
authors.forename: Shisong
authors.initials: S
authors.lastname: Fang
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Pingjian
authors.initials: P
authors.lastname: Deng
authors.forename: Shujin
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000494:pharmacology; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000187:drug effects; D019476:Insect Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D010843:Pichia / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D019476:Insect Proteins; D011994:Recombinant Proteins; C036149:cecropin D protein, Hyalophora cecropia