title: Uncoupling of flow and metabolism induced by sodium nitroprusside in rat cerebral cortex.
abstract: The effects of sodium nitroprusside (SNP) on cerebral blood flow and glucose metabolism were investigated by the microinfusion of SNP into rat cerebral cortex. A significant enhancement in glucose metabolism, as measured using [14C]deoxyglucose (DG), was observed throughout widespread areas of the cerebral cortex within 1 h of microinjection of 50 nmol/microl SNP. Using a kinetic analysis, the increase in glucose metabolism was found to be due to an increase in the phosphorylation of [14C]DG in the brain. On the other hand, regional cerebral blood flow, as measured using [14C]iodoantypirine, was not significantly altered by the SNP infusion. No significant cell death was detected by 2,3,5-triphenyltetrazolium chloride (TTC) staining 1 h after the SNP infusion. The uncoupling of flow and metabolism was almost completely prevented by pretreatment with an NMDA antagonist, MK-801. However, pretreatment with MK-801 did not prevent the SNP-induced neural cell death detected 6 h after the SNP infusion. These results suggest that the SNP-induced uncoupling of flow and metabolism was not directly related to neural cell death in the cerebral cortex.
authors.affiliation: Department of Medical Physics, School of Allied Health Sciences, Faculty of Medicine, Osaka University, 1-7 Yamadaoka, Suita-shi, Osaka 565-0871, Japan. inoue@sahs.med.osaka-u.ac.jp
authors.forename: Osamu
authors.initials: O
authors.lastname: Inoue
authors.forename: Hitomi
authors.initials: H
authors.lastname: Taguchi
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Watanabe
authors.forename: Rie
authors.initials: R
authors.lastname: Hosoi
authors.forename: Kaoru
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Tsunehiko
authors.initials: T
authors.lastname: Nishimura
authors.forename: Antony
authors.initials: A
authors.lastname: Gee
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000098:blood supply* / Q000187:drug effects* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D008297:Male; D009599:Nitroprusside / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D009599:Nitroprusside; D005947:Glucose