title: Tensile forces attenuate estrogen-stimulated collagen synthesis in the ACL.
abstract: The purpose of this study was to examine whether mechanical tensile forces affect estrogen regulation of collagen synthesis of anterior cruciate ligament fibroblasts at the mRNA level. Estrogen was studied at three physiologic levels, 10(-11), 10(-10), and 10(-9)M. The results revealed that estrogen alone stimulated Type I and III collagen synthesis at the mRNA level, and application of mechanical force decreased the expression of collagen Type I and III genes at all tested estrogen levels. These findings suggest that estrogen may directly regulate ligament structure and function by alteration of Type I and III collagen synthesis. This regulation is dependent on mechanical loading.
authors.affiliation: Department of Cell Biology, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Chun-Yi
authors.initials: CY
authors.lastname: Lee
authors.forename: Carolyn L
authors.initials: CL
authors.lastname: Smith
authors.forename: Xiaoliu
authors.initials: X
authors.lastname: Zhang
authors.forename: Horng-Chaung
authors.initials: HC
authors.lastname: Hsu
authors.forename: Der-Yean
authors.initials: DY
authors.lastname: Wang
authors.forename: Zong-Ping
authors.initials: ZP
authors.lastname: Luo
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis*; D024061:Collagen Type III / Q000096:biosynthesis*; D004967:Estrogens / Q000494:pharmacology*; D005347:Fibroblasts / Q000378:metabolism; D015870:Gene Expression; D012333:RNA, Messenger / Q000096:biosynthesis; D013314:Stress, Mechanical; D013552:Swine; D013718:Tensile Strength
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D004967:Estrogens; D012333:RNA, Messenger