title: Kidney angiotensin and angiotensin receptor expression in prenatally programmed hypertension.
abstract: Adult hypertension may be programmed by the prenatal environment in humans and in experimental animals. The potential role of the intrarenal renin-angiotensin system (RAS) in prenatally programmed hypertension was investigated. Hypertension in rat offspring was induced by maternal protein restriction during pregnancy. The offspring were studied on day 1 of life and immediately preceding the development of hypertension on day 28. ANG I and II contents were determined by radioimmunoassy. Angiotensin receptor protein and mRNA levels were quantified by immunoblotting and real-time RT-PCR, respectively. Plasma and kidney ANG I and II were unchanged in the offspring from low-protein pregnancies (LP). ANG II type 1 receptor (AT(1)R) protein abundance was low in the newborn LP kidney (P < 0.05) but rose above control values by 28 days of age (P < 0.05); the rise was associated with an increase in AT(1)R subtype A (P < 0.01), but not subtype B, mRNA level. ANG II type 2 receptor protein expression was decreased on day 1 (P < 0.05) and increased on day 28 (P < 0.05) in LP kidneys. The results show that prenatal programming of hypertension is associated with an abnormal pattern of intrarenal RAS ontogeny that may play a pathogenetic role, for instance, by constitutively altering renal hemodynamics or Na reabsorption.
authors.affiliation: The Research Institute for Children, 200 Henry Clay Ave., New Orleans, LA 70118, USA. vvehas@lsuhsc.edu
authors.forename: V Matti
authors.initials: VM
authors.lastname: Vehaskari
authors.forename: Tyrus
authors.initials: T
authors.lastname: Stewart
authors.forename: Derek
authors.initials: D
authors.lastname: Lafont
authors.forename: Christopher
authors.initials: C
authors.lastname: Soyez
authors.forename: Dale
authors.initials: D
authors.lastname: Seth
authors.forename: Jennifer
authors.initials: J
authors.lastname: Manning
mesh_terms: D000375:Aging / Q000378:metabolism; D000809:Angiotensins / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism; D018753:Diet, Protein-Restricted; D005260:Female; D006973:Hypertension / Q000209:etiology* / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics / Q000378:metabolism; D044139:Receptor, Angiotensin, Type 2 / Q000378:metabolism; D011945:Receptors, Angiotensin / Q000378:metabolism*
chemical_list: D000809:Angiotensins; D020033:Protein Isoforms; D012333:RNA, Messenger; D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2; D011945:Receptors, Angiotensin