Enzymatic nanolithography of a self-assembled oligonucleotide monolayer on gold.
This paper describes a simple strategy to biochemically manipulate a surface at the nanoscale by enzyme dip-pen nanolithography using an endonuclease (DNase I) that is directly patterned on a self-assembled monolayer presenting a terminal oligonucleotide. Physisorbed nanopatterns of DNase I carried out nanoscale enzymology at the surface creating oligonucleotide patterns with the fidelity of the patterned enzyme because of the affinity of the enzyme for the immobilized, oligonucleotide substrate.
Hyun
Jinho
J
Department of Biomedical Engineering, Center for Biologically Inspired Materials and Material Systems, Duke University, Durham, North Carolina 27708-0281, USA.
Kim
Jeonghan
J
Craig
Stephen L
SL
Chilkoti
Ashutosh
A
D003850:Deoxyribonuclease I / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods; D036103:Nanotechnology / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry*
D009841:Oligonucleotides; D006046:Gold; D003850:Deoxyribonuclease I