Increased expression of a glutamine transporter SNAT3 is a marker of malignant gliomas.
Glutamine (Gln) is a growth determinant in neoplastic tissues. We analysed by RT-PCR the expression of mRNAs coding for the human variants of Gln transporters: ASCT2 (system ASC), SNAT1 [ATA1] (system A), SNAT3 [SN1] and SNAT5 [SN2] (system N), in samples of human malignant gliomas WHO grades III/IV (anaplastic astrocytoma and glioblastoma), glioma-derived cell cultures, brain metastases from peripheral organs, and control brain tissue. SNAT3 mRNA showed a 3-5 times stronger expression in gliomas than in metastases or control tissue, and was virtually absent from glioma cultures. Native glioblastoma immunostained positively with anti-SNAT3 antibody. The expression of ASCT2 mRNA, but not SNAT5 or SNAT1 mRNAs, was increased in all neoplastic tissues studied. Hence, increased expression of SNAT3 is a marker of primary malignant gliomas in situ.
Sidoryk
Marta
M
Department of Neurotoxicology, Medical Research Centre, Polish Academy of Science, Warsaw, Poland.
Matyja
Ewa
E
Dybel
Anna
A
Zielinska
Magdalena
M
Bogucki
Jacek
J
Jaskólski
Dariusz J
DJ
Liberski
Pawel P
PP
Kowalczyk
Pawel
P
Albrecht
Jan
J
D026924:Amino Acid Transport System A / Q000235:genetics; D026962:Amino Acid Transport System ASC / Q000235:genetics; D026921:Amino Acid Transport Systems, Neutral / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D005910:Glioma / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D015778:Minor Histocompatibility Antigens; D011237:Predictive Value of Tests; D012333:RNA, Messenger / Q000378:metabolism; D015203:Reproducibility of Results; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000502:physiology
D026924:Amino Acid Transport System A; D026962:Amino Acid Transport System ASC; D026921:Amino Acid Transport Systems, Neutral; D014408:Biomarkers, Tumor; D015778:Minor Histocompatibility Antigens; D012333:RNA, Messenger; C420506:SLC1A5 protein, human; C445761:SLC38A1 protein, human; C424137:SLC38A5 protein, human; C403222:system N protein 1