Gene expression profile of human mesenchymal stem cells during osteogenesis in three-dimensional thermoreversible gelation polymer.
This study attempted to characterize the ability of thermoreversible gelation polymer (TGP) to induce differentiation of human mesenchymal stem cells (hMSC) into osteoblasts. Using a long oligo microarray system consisting of 3760 genes, we compared the expression profiles of the cells in 2-dimensional (2D) culture, 3D culture in collagen gel, and 3D culture in TGP with or without osteogenic induction. Compared to 2D culture, the gene expression profile of hMSC showed almost the same pattern in TGP without osteogenic induction, but 72% of genes (2701/3760) were up-regulated in collagen gel. With osteogenic induction, hMSC showed higher ALP activity and osteocalcin production in TGP as compared to 2D culture. Moreover, up-regulation and down-regulation of osteogenic genes were augmented in 3D culture in TGP as compared to 2D culture. As TGP is chemically synthesized and completely free from pathogen such as prion in bovine spongiform encephalopathy, these results suggest that TGP could be applied clinically to induce osteogenic differentiation of hMSC.
Hishikawa
Keiichi
K
Department of Clinical Renal Regeneration, Graduate School of Medicine, University of Tokyo, Japan. hishikawa-tky@umin.ac.jp
Miura
Shigeki
S
Marumo
Takeshi
T
Yoshioka
Hiroshi
H
Mori
Yuichi
Y
Takato
Tsuyoshi
T
Fujita
Toshiro
T
D000469:Alkaline Phosphatase / Q000378:metabolism; D001704:Biopolymers / Q000494:pharmacology; D002113:Calcification, Physiologic / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology; D015536:Down-Regulation; D005782:Gels / Q000494:pharmacology*; D020869:Gene Expression Profiling; D006358:Hot Temperature; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D015675:Osteocalcin / Q000096:biosynthesis; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D012044:Regression Analysis; D013194:Staining and Labeling / Q000379:methods; D015854:Up-Regulation
D001704:Biopolymers; D005782:Gels; D015675:Osteocalcin; D003094:Collagen; D000469:Alkaline Phosphatase