title: Phosphophloretin sensitivity of rabbit renal NaPi-IIa and NaPi-Ia.
abstract: The effect of phosphorylated phloretins on Na(+)-dependent phosphate uptake into rabbit renal brush-border membrane vesicles (BBMV) was examined. Na(+)-dependent phosphate uptake into isolated rabbit cortex BBMV was sensitive to 2'-phosphophloretin (2'-PP) and 2'-phospho-4',4,6'-trimethoxy phloretin (PTMP) in a dose-dependent and pH-dependent manner. PTMP inhibition of Na(+)-dependent phosphate uptake was maximum at alkali pH, and 2'-PP inhibition of Na(+)-dependent phosphate uptake was maximum at acidic pH. Increasing Na(+) concentrations did not increase PTMP inhibition of renal cortex BBMV Na(+)-dependent phosphate uptake at pH 6. The effect of phosphophloretins on Na(+)-dependent phosphate uptake was examined in BBMV isolated from purified proximal tubules and distal tubules. 2'-PP and PTMP inhibition of Na(+)-dependent phosphate uptake into BBMV isolated from purified proximal tubules was similar to the inhibition seen with BBMV from renal cortex. 2'-PP, but not PTMP, inhibited Na(+)-dependent phosphate uptake into BBMV isolated from purified distal tubules. The pH dependence of inhibition, the absence of PTMP inhibition of Na(+)-dependent phosphate uptake into distal tubule BBMV, and the inhibition of Na(+)-dependent phosphate uptake into distal tubule BBMV suggest that NaPi-Ia is 2'-PP sensitive and NaPi-IIa is PTMP sensitive.
authors.affiliation: Department of Physiology and Biophysics, Univesity of Texas Medical Branch, Galveston, TX 77555-0641, USA. bpeerce@utmb.edu
authors.forename: Brian E
authors.initials: BE
authors.lastname: Peerce
authors.forename: Brandon
authors.initials: B
authors.lastname: Peerce
authors.forename: Rebecca D
authors.initials: RD
authors.lastname: Clarke
mesh_terms: D000818:Animals; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007686:Kidney Tubules, Distal / Q000187:drug effects / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000378:metabolism*; D008871:Microvilli / Q000378:metabolism; D010693:Phloretin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D010710:Phosphates / Q000493:pharmacokinetics*; D010761:Phosphorus Radioisotopes; D011817:Rabbits; D012964:Sodium / Q000378:metabolism
chemical_list: C466384:2'-phosphophloretin; D010710:Phosphates; D010761:Phosphorus Radioisotopes; D012964:Sodium; D010693:Phloretin