Adoptive transfer of anti-idiotypic T cells cure mice of disseminated B cell lymphoma.
There is extensive interest in idiotypic vaccination as a treatment of lymphoma. An alternative approach is the adoptive transfer of in vitro generated T cells. This strategy has been used to treat posttransplantation EBV-related diseases. The ability to generate in vitro T cells to peptides derived from immunoglobulin idiotypes raises the possibility of directly using such cells as a treatment of lymphoma. Investigating the adoptive transfer of specific T cells to idiotype derived peptides in a murine lymphoma model is therefore an important part of the clinical translation of this alternative approach. We have generated an idiotype-specific T cell line, able to recognise a defined, naturally processed idiotype-derived epitope. This line has been used to successfully treat mice with disseminated lymphoma supporting the clinical use of idiotype specific T cells.
Armstrong
Anne C
AC
Cancer Research UK Department of Medical Oncology, Paterson Institute of Cancer Research, Christie Hospital NHS Trust, Manchester, UK.
Dermime
Said
S
Mulryan
Kate
K
Stern
Peter L
PL
Bhattacharyya
Tapan
T
Hawkins
Robert E
RE
D000256:Adenoviridae / Q000235:genetics; D019264:Adoptive Transfer / Q000379:methods*; D000818:Animals; D002455:Cell Division; D045744:Cell Line, Tumor; D002857:Chromium / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry; D005260:Female; D007128:Immunoglobulin Fragments; D007130:Immunoglobulin Idiotypes / Q000737:chemistry*; D007136:Immunoglobulins / Q000737:chemistry; D007167:Immunotherapy / Q000379:methods*; D016219:Immunotherapy, Adoptive; D007371:Interferon-gamma / Q000737:chemistry; D008223:Lymphoma / Q000276:immunology; D016393:Lymphoma, B-Cell / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D013997:Time Factors
D000939:Epitopes; D007128:Immunoglobulin Fragments; D007130:Immunoglobulin Idiotypes; D007136:Immunoglobulins; D010455:Peptides; D002857:Chromium; D007371:Interferon-gamma