Expression of CYP3A4 mRNA is correlated with CYP3A4 protein level and metabolic activity in human liver.
A relatively large amount of human liver tissue was required to determine the exact activity of human hepatic CYP3A. Although, the quantity of available human liver tissue samples is limited. We measured levels of CYP3A4 mRNA by RT-PCR with a radiolabeled primer specific for CYP3A4 and compared mRNA expression with CYP3A4 protein level and metabolic activity in liver. The level of CYP3A4 mRNA was correlated with the levels of CYP3A4 protein and activity. Our results suggest that CYP3A4 protein and activity levels can be predicted from CYP3A4 mRNA levels determined by RT-PCR and using a very small amount of liver tissue.
Watanabe
Minoru
M
Department of Pharmacology, St Marianna University School of Medicine, Kanagawa, Japan. m4wata@marianna-u.ac.jp
Kumai
Toshio
T
Matsumoto
Naoki
N
Tanaka
Masami
M
Suzuki
Satoshi
S
Satoh
Tetsuo
T
Kobayashi
Shinichi
S
D015153:Blotting, Western; D051544:Cytochrome P-450 CYP3A; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D006900:Hydroxylation; D008099:Liver / Q000201:enzymology* / Q000378:metabolism*; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis*; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013739:Testosterone / Q000032:analysis / Q000378:metabolism; D044465:White People
D012333:RNA, Messenger; D013739:Testosterone; D003577:Cytochrome P-450 Enzyme System; C104464:CYP3A protein, human; D051544:Cytochrome P-450 CYP3A; C510163:CYP3A4 protein, human