title: A "mix and read" assay for insulin using fluorometric microvolume assay technology.
abstract: We describe a novel, "mix and read" immunoassay for insulin in biological samples using FMAT. Current commercial assays for insulin require multiple washing steps and can be expensive. The insulin assay described is a simple two-step, time-saving assay and amenable to robotics. The linear response for the fluorometric signal is comparable to that observed using classical ELISA and RIA. A series of mouse plasma samples were tested for insulin levels and yielded results comparable to that measured using a commercial ELISA for insulin.
authors.affiliation: Metabolic Disorders-Diabetes, Merck Research Laboratories, Rahway, NJ 07065-0900, USA.
authors.forename: Jeffrey H
authors.initials: JH
authors.lastname: Toney
authors.forename: Aimie
authors.initials: A
authors.lastname: Ogawa
authors.forename: Megan
authors.initials: M
authors.lastname: Blair
authors.forename: Young-Whan
authors.initials: YW
authors.lastname: Park
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005470:Fluorometry / Q000191:economics / Q000379:methods*; D006801:Humans; D007328:Insulin / Q000032:analysis*; D051379:Mice; D008832:Microchemistry / Q000379:methods*; D051381:Rats
chemical_list: D007328:Insulin