Antigenic differences between H5N1 human influenza viruses isolated in 1997 and 2003.
To assess whether the antigenic properties of H5 hemagglutinin (HA) change over time due to antigenic drift, we produced a panel of monoclonal antibodies (mAbs) against the HA of the index H5N1 human influenza A virus, A/Hong Kong/156/97. By immunizing mice with a plasmid expressing this HA and boosting the initial immunization with cell lysates transfected with the plasmid, a total of six hybridomas producing HA-specific mAbs were established: four to the HA1 subunit with hemadsorption-inhibiting activity and two to the HA2 subunit. None of the mAbs to HA1 could bind to the HA of a recent human isolate, A/Hong Kong/213/2003, indicating that there are substantial antigenic differences between the H5N1 human influenza virus isolated in 1997 and that isolated in 2003.
Horimoto
Taisuke
T
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, The University of Tokyo, Japan.
Fukuda
Nao
N
Iwatsuki-Horimoto
Kiyoko
K
Guan
Yi
Y
Lim
Wilina
W
Peiris
Malik
M
Sugii
Shunji
S
Odagiri
Takato
T
Tashiro
Masato
M
Kawaoka
Yoshihiro
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D000940:Antigenic Variation / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D053124:Influenza A Virus, H5N1 Subtype; D009980:Influenza A virus / Q000276:immunology*; D051379:Mice; D010957:Plasmids / Q000276:immunology; D013997:Time Factors
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus