title: Determination of dissociation constants by competitive binding in partial filling capillary electrophoresis.
abstract: The determination of dissociation constands (K(d)) by competitive ligand binding in partial filling capillary electrophoresis is demonstrated. Two different strategies were applied, one of which only uses a single reporter ligand and a more elaborated one which suppresses systemic disturbances by using a racemic mixture as reporter. The dissociation constants obtained by both alternatives were virtually identical and in good agreement with those previously reported.
authors.affiliation: Department of Chemistry and Biomedical Sciences, University of Kalmar, Kalmar, Sweden.
authors.forename: Mikael
authors.initials: M
authors.lastname: Nilsson
authors.forename: Gunnar
authors.initials: G
authors.lastname: Johansson
authors.forename: Roland
authors.initials: R
authors.lastname: Isaksson
mesh_terms: D000526:Alprenolol / Q000378:metabolism; D001667:Binding, Competitive; D019075:Electrophoresis, Capillary / Q000379:methods*; D008689:Methacrylates / Q000378:metabolism; D011433:Propranolol / Q000378:metabolism; D012821:Silanes / Q000378:metabolism; D013237:Stereoisomerism
chemical_list: D008689:Methacrylates; D012821:Silanes; C017492:methacryloxypropyltrimethoxysilane; D000526:Alprenolol; D011433:Propranolol