title: Sil overexpression in lung cancer characterizes tumors with increased mitotic activity.
abstract: Sil (SCL interrupting locus) was cloned from the most common chromosomal rearrangement in T-cell acute lymphoblastic leukemia. It is an immediate early gene whose expression is associated with cell proliferation. Sil protein levels are tightly regulated during the cell cycle, reaching peak levels in mitosis and disappearing on transition to G1. A recent study found Sil to be one of 17 genes whose overexpression in primary adenocarcinomas predicts metastatic spread. We hypothesized that Sil might have a role in carcinogenesis. To address this question, we utilized several approaches. Using a multitumor tissue array, we found that Sil protein expression was increased mostly in lung cancer, but also at lower levels, in a subset of other tumors. Microarray gene expression analysis and immunohistochemistry of lung cancer samples verified these observations. Sil gene expression in lung cancer correlated with the expression of several kinetochore check-point genes and with the histopathologic mitotic index. These observations suggest that overexpression of the Sil gene characterizes tumors with increased mitotic activity.
authors.affiliation: Department of Pediatric Hemato-Oncology, The Chaim Sheba Cancer Research Center, Sheba Medical Center, Tel-Hashomer, Israel.
authors.forename: Ayelet
authors.initials: A
authors.lastname: Erez
authors.forename: Marina
authors.initials: M
authors.lastname: Perelman
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Hewitt
authors.forename: Gadi
authors.initials: G
authors.lastname: Cojacaru
authors.forename: Iris
authors.initials: I
authors.lastname: Goldberg
authors.forename: Iris
authors.initials: I
authors.lastname: Shahar
authors.forename: Pnina
authors.initials: P
authors.lastname: Yaron
authors.forename: Inna
authors.initials: I
authors.lastname: Muler
authors.forename: Stefano
authors.initials: S
authors.lastname: Campaner
authors.forename: Ninette
authors.initials: N
authors.lastname: Amariglio
authors.forename: Gideon
authors.initials: G
authors.lastname: Rechavi
authors.forename: Ilan R
authors.initials: IR
authors.lastname: Kirsch
authors.forename: Meir
authors.initials: M
authors.lastname: Krupsky
authors.forename: Naftali
authors.initials: N
authors.lastname: Kaminski
authors.forename: Shai
authors.initials: S
authors.lastname: Izraeli
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism; D015153:Blotting, Western; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D016193:G1 Phase; D017781:Genes, Immediate-Early; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins; D018386:Kinetochores / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism*; D008938:Mitosis; D009362:Neoplasm Metastasis; D020411:Oligonucleotide Array Sequence Analysis; D015514:Oncogene Proteins, Fusion / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D015514:Oncogene Proteins, Fusion; D012333:RNA, Messenger; C071236:STIL protein, human