title: [Screening of schistosomiasis japonica diagnostic antigen with phage 12-mer peptide library].
abstract: OBJECTIVE
  To obtain the mimic epitope of specific and sensitive diagnostic antigen in schistosomiasis japonica from phage 12-mer peptide library.
  
  
  METHODS
  Specific Ig was purified from sera of patients with acute schistosomiasis and used to immunoscreen the phage peptide library (PH. D.-12). After 3 rounds of panning, 10 positive plaques were selected and amplified. The immunoactivity of each clone was examined by ELISA. The sensitivity and specificity of immunoactive clones were confirmed by detecting the sera of patients with different parasitosis.
  
  
  RESULTS
  Six clones could bind to the specific Ig purified from sera of patients with acute schistosomiasis. One clone with the highest A492 value showed a high sensitivity and specificity.
  
  
  CONCLUSIONS
  The clone (SjA1) identified by the specific Ig from the library played a better part in the immunodiagnosis of schistosomiasis.
authors.affiliation: Department of Pathogenic Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
authors.forename: Xiao-hua
authors.initials: XH
authors.lastname: Zhu
authors.forename: Chang-fu
authors.initials: CF
authors.lastname: Jiang
authors.forename: Lan-ying
authors.initials: LY
authors.lastname: Wei
authors.forename: Jia-hui
authors.initials: JH
authors.lastname: Lei
authors.forename: Hong-bo
authors.initials: HB
authors.lastname: Shi
authors.forename: Hong
authors.initials: H
authors.lastname: Pan
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology; D019151:Peptide Library; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000175:diagnosis / Q000276:immunology*; D012680:Sensitivity and Specificity
chemical_list: D000947:Antigens, Helminth; D000939:Epitopes; D007136:Immunoglobulins; D019151:Peptide Library