Circadian regulation of GABAA receptor function by CKI epsilon-CKI delta in the rat suprachiasmatic nuclei.
The type A GABA receptors are thought to mediate synchronization of clock cell activity within the suprachiasmatic nuclei (SCN). Here we report that casein kinases I epsilon and delta (CKI epsilon and CKI delta), the crucial clock regulators, form a complex with GABA(A) receptors and inhibit the receptors' function within the SCN according to a circadian rhythm. These results indicate that circadian variation of the kinase-receptor association may mediate regulation of GABA(A) receptor function by CKI epsilon-CKI delta in the SCN.
Ning
Ke
K
Division of Cellular & Molecular Biology, Toronto Western Research Institute, University Health Network, and Department of Physiology, University of Toronto, Toronto, Ontario, Canada.
Li
Lei
L
Liao
Mingxia
M
Liu
Baosong
B
Mielke
John G
JG
Chen
Yonghong
Y
Duan
Ying
Y
El-Hayek
Youssef H
YH
Wan
Qi
Q
D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D047388:Casein Kinases; D002940:Circadian Rhythm / Q000502:physiology*; D003624:Darkness; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007150:Immunohistochemistry / Q000379:methods; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology; D008029:Lighting / Q000379:methods; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000528:radiation effects; D018408:Patch-Clamp Techniques / Q000379:methods; D010696:Phloroglucinol / Q000031:analogs & derivatives* / Q000494:pharmacology; D011233:Precipitin Tests / Q000379:methods; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000502:physiology*; D018080:Receptors, GABA-B / Q000378:metabolism; D013493:Suprachiasmatic Nucleus / Q000502:physiology*; D013997:Time Factors
D004791:Enzyme Inhibitors; C411652:IC 261; D007211:Indoles; D011963:Receptors, GABA-A; D018080:Receptors, GABA-B; D010696:Phloroglucinol; D011494:Protein Kinases; D047388:Casein Kinases