The inhibition of nitric oxide synthase enhances PSA-NCAM expression and CREB phosphorylation in the rat hippocampus.
It is well known that nitric oxide (NO) acts downstream of NMDA receptor activation, which regulates the neural plasticity in the brain. In the present study, the effect of L-NAME, a non-selective nitric oxide synthase (NOS) inhibitor, on neural plasticity in the hippocampus was investigated. L-NAME increased the expression of PSA-NCAM and pCREB in the adult rat hippocampus. The co-localization of PSA-NCAM and pCREB indicates a possible relationship between the two in the granule cell layer in the dentate gyrus. Our results demonstrate that NO, as a subsignal of NMDA receptors, could be involved in the structural plasticity of the granule cell layer in the dentate gyrus by regulating the expression of PSA-NCAM and pCREB in the hippocampus.
Park
Chan
C
Department of Anatomy, Medical Science and Engineering Research Center for Reactive Oxygen Species, College of Medicine, Kyunghee University, Hoeki-Dong 1, Dongdaemun-Gu, Seoul 130-701, South Korea.
Shin
Ki Soon
KS
Ryu
Jong Hoon
JH
Kang
Kyounglan
K
Kim
Junghye
J
Ahn
Heekyung
H
Huh
Youngbuhm
Y
D000818:Animals; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D018891:Dentate Gyrus / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D039842:Neural Cell Adhesion Molecule L1 / Q000378:metabolism*; D009434:Neural Pathways / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D009473:Neuronal Plasticity / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000201:enzymology; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012794:Sialic Acids / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
D017362:Cyclic AMP Response Element-Binding Protein; D004791:Enzyme Inhibitors; D039842:Neural Cell Adhesion Molecule L1; D012794:Sialic Acids; C103469:polysialyl neural cell adhesion molecule; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester