title: Functional CCR9 expression is associated with small intestinal metastasis.
abstract: In general, metastases to the small intestine are rare, and mostly occur in melanoma. CCR9 has been shown to be the principal chemokine receptor for the thymus expressed chemokine (TECK), a chemokine selectively expressed in the small intestine and thymus. Here we show that CCR9 is highly expressed on melanoma cells and all melanoma cell lines isolated from small intestinal metastases, and on a proportion of cell lines from other sites. Only melanoma cells and cell lines from small intestinal metastases, however, were responsive to the CCR9 ligand TECK, as assessed by receptor downregulation and by actin polymerization. CCR9 expression was also found on the adenocarcinoma cell line CaCo-2 expressing characteristics of enterocytic differentiation, but not on any other cell line isolated from colorectal, breast, and lung cancer. Our data provide evidence that the aberrant functional cell surface expression of an organ-specific chemokine receptor is associated with metastasis to this site. The regulation of receptor function seems to be a critical step in the metastatic process.
authors.affiliation: Charité University Medicine Berlin, Campus Benjamin-Franklin, Hematology, Oncology and Transfusion Medicine, Berlin, Germany. anne.letsch@medizin.fu-berlin.de
authors.forename: Anne
authors.initials: A
authors.lastname: Letsch
authors.forename: Ulrich
authors.initials: U
authors.lastname: Keilholz
authors.forename: Dirk
authors.initials: D
authors.lastname: Schadendorf
authors.forename: Geraldine
authors.initials: G
authors.lastname: Assfalg
authors.forename: Anne Marie
authors.initials: AM
authors.lastname: Asemissen
authors.forename: Eckhard
authors.initials: E
authors.lastname: Thiel
authors.forename: Carmen
authors.initials: C
authors.lastname: Scheibenbogen
mesh_terms: D000199:Actins / Q000378:metabolism; D018938:Caco-2 Cells; D004379:Duodenal Neoplasms / Q000276:immunology / Q000556:secondary*; D006801:Humans; D007078:Ileal Neoplasms / Q000276:immunology / Q000556:secondary*; D007580:Jejunal Neoplasms / Q000276:immunology / Q000556:secondary*; D008545:Melanoma / Q000276:immunology / Q000556:secondary*; D054388:Receptors, CCR; D054400:Receptors, CCR7; D019718:Receptors, CXCR4 / Q000032:analysis; D019707:Receptors, Chemokine / Q000032:analysis* / Q000502:physiology
chemical_list: D000199:Actins; C119309:CC chemokine receptor 9; C515370:CCR7 protein, human; D054388:Receptors, CCR; D054400:Receptors, CCR7; D019718:Receptors, CXCR4; D019707:Receptors, Chemokine