Delivery of short hairpin RNA sequences by using a replication-competent avian retroviral vector.
While recent studies have demonstrated that retroviral vectors can be used to stably express short hairpin RNA (shRNA) to inhibit gene expression, these studies have utilized replication-defective retroviruses. We describe the creation of a replication-competent, Gateway-compatible retroviral vector capable of expressing shRNA that inhibits the expression of specific genes.
Bromberg-White
Jennifer L
JL
Laboratory of Tumor Metastasis and Angiogenesis, Van Andel Research Institute, Grand Rapids, Michigan 49503, USA.
Webb
Craig P
CP
Patacsil
Veronique S
VS
Miranti
Cindy K
CK
Williams
Bart O
BO
Holmen
Sheri L
SL
D000818:Animals; D001717:Birds / Q000821:virology*; D002460:Cell Line; D045744:Cell Line, Tumor; D005347:Fibroblasts; D005822:Genetic Vectors; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication
D034741:RNA, Small Interfering; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases