[Expression and identification of cecropin D gene cloned in Pichia pastoris yeast cells].
OBJECTIVE
Cecropin D gene was cloned into methanol nutritional vector-pPICZ alpha-A, linked with alpha-factor signal peptide.


METHODS
Positive recombinant was gotten through PCR and EcoR I restriction enzyme. The positive recombinant vector was transformed into Pichia pastoris yeast strain GS115.


RESULTS
The positive recombinant yeast velum was fermented and its expressive product was assayed by the method of anti-germ.


CONCLUSIONS
Cecropin D gene was cloned into yeast cell and its expressive products has high activity of killing bacteria, Which could be developed into a new type of anti-bacterial medicine.
Fang
Shisong
S
South China University of Technology, Guangzhou 510642, China.
Liu
Tao
T
Deng
Pingjian
P
Zhao
Shujin
S
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000494:pharmacology; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000187:drug effects; D019476:Insect Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D010843:Pichia / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014170:Transformation, Genetic
D000900:Anti-Bacterial Agents; D019476:Insect Proteins; D011994:Recombinant Proteins; C036149:cecropin D protein, Hyalophora cecropia