Methods of analyzing chromatin changes accompanying apoptosis of target cells in killer cell assays.
Natural killer (NK) cells are a crucial component of the immune system. For example, the NK cell-mediated killing of tumor cells is a first line of defense against the development of cancer. Detection and quantification of apoptosis induced in target cells by NK cells is a useful tool for studies of the mechanisms of both immunological protection and pathogenesis. This chapter describes how to do this by using techniques with a broad application to both NK cells and cytotoxic T cells.
Duke
Richard C
RC
CERES Pharmaceuticals Ltd., Denver, CO, USA.
D017209:Apoptosis / Q000502:physiology*; D001681:Biological Assay / Q000379:methods*; D002614:Chelating Agents / Q000378:metabolism; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism*; D053938:DNA Fragmentation; D004533:Egtazic Acid / Q000378:metabolism; D053222:Fas Ligand Protein; D005456:Fluorescent Dyes / Q000378:metabolism; D020287:In Situ Nick-End Labeling; D015759:Ionomycin / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000378:metabolism; D008853:Microscopy / Q000379:methods; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014466:Ultraviolet Rays; D019014:fas Receptor / Q000378:metabolism
D002614:Chelating Agents; D002843:Chromatin; D053222:Fas Ligand Protein; D005456:Fluorescent Dyes; D007476:Ionophores; D008562:Membrane Glycoproteins; D019014:fas Receptor; D004533:Egtazic Acid; D015759:Ionomycin; D013755:Tetradecanoylphorbol Acetate