[Pigments of green sulfur bacteria isolated from reservoirs of Iavoriv sulfur deposit].
The enormous amount of hydrogen sulfide (up to 11 mg/ml) is present in the Yavoriv sulfur deposit reservoirs owing to sulfur reductive bacteria activity. As a consequence the ecological situation is badly affected and requires recovering. The biological H2S decomposition by photosynthetic sulfur bacteria, which use the hydrogen sulfide as electron donor during photosynthesis, can be one of the possible ways of this toxic substance destruction. The qualitative and quantitative analysis of photosynthetic pigments composition that derived from green photosynthesizing sulfur bacteria from reservoirs of Yavoriv sulfur deposit is carried out. It was fixed that Pelodictyon sp., Chlorobium sp. and isolated consortia "Pelochromatium sp." contain the bacteriochlorophyll c and d. All the isolated cultures contained bacteriochlorophyll a in trace amounts. The obtained photosynthetic pigments (bacteriochlorophylls, carotenoids) were recognized by their absorption spectra in the visible and far-red region and by their quantity. The difference was not essential. All investigated cultures of isolated bacteria contain some carotenoid the Chlorobium sp. and obtained consortia possesses isorenieratene. The absorption maxima of extracted pigments from young cultures of isolated green sulfur bacteria are more definitely displayed than those from old cultures. Investigations of phototrophic sulfur bacteria were carried out in Ukraine up to now. Ecological problem that occurred in the Yavoriv sulfur deposit as a result of the deposit exploitation caused a necessity of the investigation of photosynthetic sulfur bacteria and bacterial photosynthesis mechanism. The photosynthetic pigments nature identification will promote the fast and precise identification of the new forms of photosynthetic sulfur bacteria and will extend our knowledge about their role in the anoxygenic photosynthesis.
Baran
I M
IM
Ivan Franko Lviv National University, 4 Hrushevskogo St., Lviv, 79005, Ukraine.
Hudz'
S P
SP
Hnatush
S O
SO
Fedorovych
A M
AM
D001426:Bacterial Proteins / Q000032:analysis; D001429:Bacteriochlorophylls / Q000032:analysis; D002338:Carotenoids / Q000032:analysis; D019414:Chlorobi / Q000737:chemistry* / Q000254:growth & development / Q000302:isolation & purification; D004783:Environmental Microbiology; D010788:Photosynthesis; D010860:Pigments, Biological / Q000032:analysis*; D013053:Spectrophotometry; D014455:Ukraine
D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D010860:Pigments, Biological; D002338:Carotenoids; C002084:bacteriochlorophyll c; C459779:bacteriochlorophyll d