Differentiation pattern of Vero cells cultured on poly(L-lactic acid)/poly(hydroxybutyrate-co-hydroxyvalerate) blends.
This study evaluates the effect of poly(L-lactic acid) (PLLA) and poly(hydroxybutyrate-cohydroxyvalerate) (PHBV) bioabsorbable polymers and their blends on the induction of alteration of cell growth pattern in vitro. Vero cells were cultured on PLLA, PHBV, and different blends (100/0, 60/40, 50/50, 40/60, and 0/100). The cell adhesion assay showed that the best results were obtained with the (60/40, 50/50) blends. Scanning electron microscopy showed that the cells on (100/0) and (60/40) samples grew with a round morphology preferentially in the porous areas. The (50/50) blends had cells in the porous and smooth areas in a similar way. The (40/60) blends showed spreading cells on the smooth areas. The (0/100) sample, which had no pores, had spreading cells interconnected by filaments. Histological sections showed a confluent cell monolayer and the immunocytochemistry showed that the cells produced collagen IV and fibronectin on all substrates. Thus, we conclude that PLLA/PHBV blends were efficient in maintaining cell growth and producing an extracellular matrix on them.
Santos
Arnaldo R
AR
Department of Cell Biology, Institute of Biology, State University of Campinas, Campinas, SP, Brazil. arsantosjr@hotmail.com
Ferreira
Betina M P
BM
Duek
Eliana A R
EA
Dolder
Heidi
H
Wada
Ronaldo S
RS
Wada
Maria Lucia F
ML
D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D024141:Collagen Type IV / Q000096:biosynthesis; D005353:Fibronectins / Q000096:biosynthesis; D006885:Hydroxybutyrates / Q000737:chemistry*; D007150:Immunohistochemistry; D019344:Lactic Acid / Q000737:chemistry*; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D014631:Valerates / Q000737:chemistry*; D014709:Vero Cells
D001672:Biocompatible Materials; D024141:Collagen Type IV; D005353:Fibronectins; D006885:Hydroxybutyrates; D008567:Membranes, Artificial; D011091:Polyesters; D011108:Polymers; D014631:Valerates; D019344:Lactic Acid; C033616:poly(lactide)