Isolation of a putative ribosome inactivating protein from dried roots of Trichosanthes kirilowii used in Traditional Chinese Medicine.
A putative ribosome inactivating protein (RIP), named T 33, was isolated from dried roots of Trichosanthes kirilowii Maximovicz which are used in Traditional Chinese Medicine. After chromatographic enrichment a protein band with molecular mass of 33 kDa was found by SDS-PAGE. Partial amino acid sequences of this protein consisting of 8 - 33 amino acids reveal homology to highly conserved regions of different RIPs, but also indicate T 33 to be a novel type 2 RIP. Future heterologous expression of this protein will allow investigation of its biological activities in detail.
Fei
Xu
X
College of Life Science, Jilin University, Changchun, P. R. China.
Hill
Marco
M
Ma
Xueyan
X
Teng
Lirong
L
Li
Qingshan
Q
Stöckigt
Joachim
J
D000595:Amino Acid Sequence; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008969:Molecular Sequence Data; D008517:Phytotherapy; D010940:Plant Proteins / Q000032:analysis / Q000302:isolation & purification*; D018517:Plant Roots; D011500:Protein Synthesis Inhibitors / Q000032:analysis / Q000302:isolation & purification*; D029778:Trichosanthes
D004365:Drugs, Chinese Herbal; D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors