title: A sol-gel method using acetic anhydride in the presence of cholesterol in organic solution media: preparation of silicas that recognize steroid hormones.
abstract: Silicas were prepared by a sol-gel method from tetraethoxysilane and acetic anhydride in the presence of cholesterol in organic solution media. Some silicas had higher pore volumes than silicas obtained in the absence of cholesterol. The adsorption abilities by these silicas were compared using various compounds in benzene solution. Although no clear difference among their adsorptions of cholesterol was observed, progesterone and other analogous steroid hormones were well adsorbed by silicas prepared in the presence of cholesterol, especially, prepared with n-heptane as an additional solvent. This silica adsorbed steroid hormones more selectively than other analogous compounds such as bisphenol A and hexestrol. On the other hand, a silica prepared by the usual aqueous sol-gel method with cholesterol had no clear adsorption selectivity to steroid hormones. Furthermore, no selective adsorption of steroid hormones was observed in the case of a common silica gel for column chromatography. This unique property of adsorption observed in silicas prepared using acetic anhydride in the presence of cholesterol is likely to be induced by the imprinting effect of the steroid skeleton part of cholesterol in silica matrix.
authors.affiliation: Kansai Center, National Institute of Advanced Industrial Science and Technology (AIST-Kansai), 1 Midorigaoka, Ikeda, Osaka 563-8577, Japan. m-fujiwara@aist.go.jp
authors.forename: Masahiro
authors.initials: M
authors.lastname: Fujiwara
authors.forename: Motoyasu
authors.initials: M
authors.lastname: Nishiyama
authors.forename: Ikou
authors.initials: I
authors.lastname: Yamamura
authors.forename: Souichi
authors.initials: S
authors.lastname: Ohtsuki
authors.forename: Ryoki
authors.initials: R
authors.lastname: Nomura
mesh_terms: D000089:Acetic Anhydrides / Q000737:chemistry*; D000327:Adsorption; D002784:Cholesterol / Q000737:chemistry*; D005782:Gels; D015394:Molecular Structure; D009930:Organic Chemicals / Q000737:chemistry; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry*; D013256:Steroids / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000089:Acetic Anhydrides; D005782:Gels; D009930:Organic Chemicals; D012821:Silanes; D012996:Solutions; D013256:Steroids; C031800:acetic anhydride; C040733:tetraethoxysilane; D012822:Silicon Dioxide; D002784:Cholesterol