title: [Preliminary analysis on the gene expression profiles of medulloblastomas by use of cDNA array].
abstract: OBJECTIVE
  To explore the molecular genesis of medulloblastomas with cDNA array.
  
  
  METHODS
  Four samples of medulloblastomas and 1 sample of normal brain tissue were collected freshly. After total RNA extraction, the (32)P targeted cDNA probes were converted and then hybridized with Atlas Human Cancer Array 1.2. The gene expression profiles were acquired through autoradiography. The discrepancy between the tumor and the normal brain tissue was analyzed with Atlas Image 1.01a.
  
  
  RESULTS
  In comparison with the genes in the normal brain tissue, 6 down-regulated and 35 up-regulated genes in the medulloblastomas were revealed by means of the microarrays and autoradiography, and were verified by reverse transcriptase-PCR. The regulatory trends of most differential expression genes were in compliance with the biological features of this tumor.
  
  
  CONCLUSIONS
  Medulloblastomas are diseases involving multiple genes with some molecular pathological mechanisms different from the astrocytic gliomas. There are complex interrelationships between these genes, which need to be further researched.
authors.affiliation: Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, 300052 PR China.
authors.forename: Rong-cai
authors.initials: RC
authors.lastname: Jiang
authors.forename: Pei-yu
authors.initials: PY
authors.lastname: Pu
authors.forename: Chang-hong
authors.initials: CH
authors.lastname: Shen
authors.forename: Shiz-hu
authors.initials: SH
authors.lastname: Yu
authors.forename: Bao-hua
authors.initials: BH
authors.lastname: Jiao
authors.forename: Hu
authors.initials: H
authors.lastname: Wang
authors.forename: Chun-yan
authors.initials: CY
authors.lastname: Wang
authors.forename: Chun-sheng
authors.initials: CS
authors.lastname: Kang
mesh_terms: D002648:Child; D002675:Child, Preschool; D020869:Gene Expression Profiling; D006801:Humans; D008527:Medulloblastoma / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis