title: Continuous-flow UVC irradiation: a new, effective, protein activity-preserving system for inactivating bacteria and viruses, including erythrovirus B19.
abstract: Despite the increasing number of screening tests being introduced, ensuring the inactivation of blood-borne pathogens in blood-derived therapeutic material is a major concern. Dynamic continuous-flow UVC irradiation is a new way to inactivate a large range of pathogens without adding any photosentizers. The efficacy of different methods was evaluated against the following viruses: murine parvovirus MVMp, human B19, the encephalomyocarditis virus (EMC, a picornavirus used as a model for model for hepatitis A virus), and bovine herpes virus type 1 (BHV, a model for enveloped viruses such as hepatitis B virus). We show that continuous-flow UVC irradiation is very effective, particularly against resistant pathogens (e.g. parvoviruses and bacteria) at UVC doses preserving protein activity. It may be applicable to newly emerging related viruses or variants.
authors.affiliation: Laboratoire de Virologie Moléculaire, Faculté de Médecine, Université Libre de Bruxelles, CP614, 808 route de Lennik, 1070 Brussels, Belgium. pcaillet@ulb.ac.be
authors.forename: Perrine
authors.initials: P
authors.lastname: Caillet-Fauquet
authors.forename: Mario
authors.initials: M
authors.lastname: Di Giambattista
authors.forename: Marie-Louise
authors.initials: ML
authors.lastname: Draps
authors.forename: Flavienne
authors.initials: F
authors.lastname: Sandras
authors.forename: Theo
authors.initials: T
authors.lastname: Branckaert
authors.forename: Yvan
authors.initials: Y
authors.lastname: de Launoit
authors.forename: Ruth
authors.initials: R
authors.lastname: Laub
mesh_terms: D000818:Animals; D001419:Bacteria / Q000528:radiation effects*; D001798:Blood Proteins / Q000378:metabolism / Q000528:radiation effects; D017848:Blood-Borne Pathogens / Q000528:radiation effects*; D002417:Cattle; D004203:Disinfection / Q000379:methods*; D005169:Factor VIII / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D051379:Mice; D016732:Parvovirus B19, Human / Q000528:radiation effects; D010949:Plasma / Q000528:radiation effects; D014466:Ultraviolet Rays; D014780:Viruses / Q000528:radiation effects*
chemical_list: D001798:Blood Proteins; D007136:Immunoglobulins; D005169:Factor VIII; D005340:Fibrinogen