Engineered endophytic bacteria improve phytoremediation of water-soluble, volatile, organic pollutants.
Phytoremediation of highly water soluble and volatile organic xenobiotics is often inefficient because plants do not completely degrade these compounds through their rhizospheres. This results in phytotoxicity and/or volatilization of chemicals through the leaves, which can cause additional environmental problems. We demonstrate that endophytic bacteria equipped with the appropriate degradation pathway improve the in planta degradation of toluene. We introduced the pTOM toluene-degradation plasmid of Burkholderia cepacia G4 into B. cepacia L.S.2.4, a natural endophyte of yellow lupine. After surface-sterilized lupine seeds were successfully inoculated with the recombinant strain, the engineered endophytic bacteria strongly degraded toluene, resulting in a marked decrease in its phytotoxicity, and a 50-70% reduction of its evapotranspiration through the leaves. This strategy promises to improve the efficiency of phytoremediating volatile organic contaminants.
Barac
Tanja
T
Limburgs Universitair Centrum (LUC), Department of Environmental Biology, Universitaire Campus building D, B-3590 Diepenbeek, Belgium.
Taghavi
Safiyh
S
Borremans
Brigitte
B
Provoost
Ann
A
Oeyen
Licy
L
Colpaert
Jan V
JV
Vangronsveld
Jaco
J
van der Lelie
Daniel
D
D001673:Biodegradation, Environmental; D016956:Burkholderia cepacia / Q000235:genetics / Q000378:metabolism*; D030781:Organisms, Genetically Modified; D012995:Solubility; D014835:Volatilization; D014867:Water; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D014874:Water Pollutants, Chemical; D014867:Water