[The internal control role of ribosomal protein S7 in the defense of Anopheles dirus against Plasmodium infection].
OBJECTIVE
To investigate the role of ribosomal protein S7 (rpS7) in the defense of Anopheles dirus against infection.


METHODS
rpS7 was amplified from Anopheles dirus hemocytes with degenerated primers designed according to the conservative region of S7, rpS7 was then cloned using T/A cloning kit and the inserted fragment was sequenced. The difference of the transcript abundance of rpS7 from Anopheles dirus hemocyte among non-blood-fed (N), normal-blood-fed (B) and Plasmodium yoelii infected groups (I) was also analyzed by RT-PCR and gel scanning system at d1, d2, d3, d4, d7 and d11 after blood feeding.


RESULTS
There is no significant difference of rpS7 signal between the three groups.


CONCLUSIONS
Anopheles dirus S7 can be used as an internal control to study the role of Anopheles dirus related immune factors in Plasmodium infection.
Xu
Wen-yue
WY
Department of Pathogenic Biology, Third Military Medical University, Chongqing, 400038.
Huang
Fu-sheng
FS
Duan
Jian-hua
JH
D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000235:genetics / Q000469:parasitology*; D001483:Base Sequence; D005260:Female; D007303:Insect Vectors / Q000235:genetics / Q000469:parasitology*; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D010967:Plasmodium yoelii / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000235:genetics / Q000502:physiology*; D017386:Sequence Homology, Amino Acid
D012269:Ribosomal Proteins; C037442:ribosomal protein S7