title: Chemiluminescence quenching of pteroic acid-N-sulfonyl-acridinium-9-carboxamide conjugates by folate binding protein.
abstract: N(10)-Trifluoroacetylpteroic acid was conjugated to chemiluminescent N-sulfonylacridinium-9-carboxamide labels at the N(10) or 9-position carboxamide. Upon binding to folate binding protein the light output of the N(10) derivative (9) was quenched up to 62% upon triggering with basic peroxide, while the 9-position carboxamide conjugate (7) was quenched only 12%. The utility of this effect was demonstrated in a model homogeneous chemiluminescent assay for folic acid.
authors.affiliation: Department of Chemistry (D09MD), Abbott Laboratories, 100 Abbott Park Road, AP20, Abbott Park, IL 60064-6016, USA. maciej.adamczyk@abbott.com
authors.forename: Maciej
authors.initials: M
authors.lastname: Adamczyk
authors.forename: James R
authors.initials: JR
authors.lastname: Fino
authors.forename: Phillip G
authors.initials: PG
authors.lastname: Mattingly
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Moore
authors.forename: You
authors.initials: Y
authors.lastname: Pan
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry*; D058973:Folate Receptors, GPI-Anchored; D049449:Luminescence; D011956:Receptors, Cell Surface / Q000737:chemistry*
chemical_list: D002352:Carrier Proteins; D058973:Folate Receptors, GPI-Anchored; D011956:Receptors, Cell Surface