Cell cycle-dependent dynamic localization of a bacterial response regulator with a novel di-guanylate cyclase output domain.
Pole development is coordinated with the Caulobacter crescentus cell cycle by two-component signaling proteins. We show that an unusual response regulator, PleD, is required for polar differentiation and is sequestered to the cell pole only when it is activated by phosphorylation. Dynamic localization of PleD to the cell pole provides a mechanism to temporally and spatially control the signaling output of PleD during development. Targeting of PleD to the cell pole is coupled to the activation of a C-terminal guanylate cyclase domain, which catalyzes the synthesis of cyclic di-guanosine monophosphate. We propose that the local action of this novel-type guanylate cyclase might constitute a general regulatory principle in bacterial growth and development.
Paul
Ralf
R
Division of Molecular Microbiology, Biozentrum, University of Basel, 4056 Basel, Switzerland.
Weiser
Stefan
S
Amiot
Nicholas C
NC
Chan
Carmen
C
Schirmer
Tilman
T
Giese
Bernd
B
Jenal
Urs
U
D001426:Bacterial Proteins / Q000378:metabolism*; D016935:Caulobacter crescentus / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology*; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000096:biosynthesis; D006160:Guanosine Triphosphate / Q000378:metabolism; D006162:Guanylate Cyclase / Q000378:metabolism*; D000071677:Histidine Kinase; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism
D001426:Bacterial Proteins; C096455:PleD protein, Caulobacter crescentus; C062025:bis(3',5')-cyclic diguanylic acid; D006160:Guanosine Triphosphate; D011494:Protein Kinases; D000071677:Histidine Kinase; D006162:Guanylate Cyclase; D006152:Cyclic GMP