Inhibition of tumorigenicity of the hepatitis B virus X gene in Chang liver cell line.
The hepatitis B virus X gene, which encodes the HBx protein, has multiple functions and is involved in hepatocarcinogenesis. However, the exact role of HBx in hepatocarcinogenesis is still controversial. We have established an inducible (tet-off system) HBx-expressing cell line, Chang-HBx. Compared with the original of Chang liver cell line (ATCC CCL13), Chang-HBx grows faster in serum-containing medium but slower in serum-free medium. Chang-HBx colony formation in soft agar shows an anchorage-demanding character and its tumorigenicity potential in BALB/c nude mice were substantially inhibited. HBx also causes the induction of G1 phase arrest of cell growth in early infection of HBV and therefore plays a negative role in tumorigenicity. An excellent mice animal model for producing hepatoma was also provided in this study.
Wang
Jing-Chyi
JC
Department of Biology, Tunghai University, 181, Sec. 3, Chungkang Road, Taichung, Taiwan, ROC.
Hsu
Shih-Lan
SL
Hwang
Guang-Yuh
GY
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D015870:Gene Expression; D005814:Genes, Viral; D006515:Hepatitis B virus / Q000235:genetics*; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism / Q000821:virology; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000821:virology; D051379:Mice; D008819:Mice, Nude; D010957:Plasmids; D015534:Trans-Activators / Q000235:genetics* / Q000502:physiology*; D054334:Viral Regulatory and Accessory Proteins
D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein