Tight binding ligand approach to oligosaccharide-grafted protein.
A novel type of artificial glycoprotein was developed, by using dihydrofolate reductase (DHFR) and methotrexate (MTX) as a protein-ligand pair. Various oligosaccharides linked to MTX were shown to bind tightly with DHFR and afforded oligosaccharide-grafted protein, which could be isolated easily by lectin beads.
Totani
Kiichiro
K
CREST, JST, Kawaguchi 332-1102, Japan.
Matsuo
Ichiro
I
Ito
Yukishige
Y
D002240:Carbohydrate Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D008024:Ligands; D008727:Methotrexate / Q000737:chemistry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry / Q000378:metabolism*
D008024:Ligands; D009844:Oligosaccharides; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate