Human oocytes and preimplantation embryos express mRNA for growth hormone receptor.
Human genetic expression of growth hormone receptor (GHR) gene was qualitatively analysed using reverse transcription polymerase chain reaction (RT-PCR) in cumulus cells, immature germinal vesicle (GV) and mature metaphase II (MII) stage oocytes and preimplantation human embryos. The transcripts encoding GHR were detected in cumulus cells and also in naked oocytes, either mature or not. In this case, a nested PCR is needed, as for early embryo preimplantation stages, before genomic activation. The GHR gene is highly expressed from the 4-day morula onwards. This suggests that GHR transcription follows a classical scheme associated with genomic activation. It is probable that, in human, growth hormone plays a role in the final stages of oocyte maturation and early embryogenesis as it does for several other mammalian species.
Ménézo
Y J
YJ
Laboratoire Marcel Merieux, 1 Rue Laborde, 69500 Bron, France. ymenezo@lab-merieux.fr
el Mouatassim
S
S
Chavrier
M
M
Servy
E J
EJ
Nicolet
B
B
D001755:Blastocyst / Q000378:metabolism*; D017931:DNA Primers; D005260:Female; D006801:Humans; D008677:Metaphase / Q000502:physiology; D009865:Oocytes / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D011986:Receptors, Somatotropin / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D012333:RNA, Messenger; D011986:Receptors, Somatotropin