Flow cytometric measurement of intracellular IFN-gamma induction in aged subjects before and after parenteral influenza vaccination.
Flow cytometric analysis, used to study intracellular expression of IFN-gamma in peripheral blood mononuclear cells (PBMC) from aged volunteers before and after parenteral influenza vaccination, was found capable of rapidly detecting influenza antigen induced variation of IFN-gamma expression. Although the vaccine was capable of generating a satisfactory antibody response, it did not stimulate an increase in the percentage of IFN-gamma positive cells.
Fringuelli
E
E
Department of Hygiene and Public Health, University of Perugia, Italy.
Lepri
E
E
Camilloni
B
B
Neri
M
M
Carriò
E
E
Russano
A
A
Spinozzi
F
F
Iorio
A M
AM
D000368:Aged; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D007371:Interferon-gamma / Q000032:analysis / Q000096:biosynthesis*
D007252:Influenza Vaccines; D007371:Interferon-gamma