[Study on the teratogenicity effects of genetically modified rich which expressed cowpea trypsin inhibitor on rats].
OBJECTIVE
Rats were fed by transgenic rice which expressed insecticidal protein CpTI (cowpea trypsin inhibitor) to study if the transgenic rice possessed potential teratogenicity effects.


METHODS
Weanling Wistar rats were randomly divided into four groups: transgenic rice group, non transgenic rice group, negative control group and positive control group. The diet of transgenic rice group contained 78.3% transgenic rice. The diet of non transgenic rice group contained 74.7% non transgenic rice which was the parent line of the transgenic one. The diet formula of the two control groups was AIN93G. The marco- and micronutrient content were equal in three diets. When the sexual maturation period of rats arrived, conventional teratogenicity test was performed.


RESULTS
The body weight gain of pregnant rats and body weight, body length and tail length of fetal rats in transgenic rice group were significant higher than those in positive control group (P < 0.01). The malformation rate of fetus in transgenic rice group was significant lower than that in positive control group (P < 0.01).


CONCLUSIONS
There were no significant differences of all indexes among transgenic rice group, non transgenic rice group and negative control group (P > 0.05). Judging from these results, the transgenic rice modified with CpTI was considered to have no maternal toxicity, embryotoxicity and teratogenicity effects.
Zhuo
Qin
Q
Institute of Nutrition and Food Safety, Chinese Centre for Diease Control and Prevention, Beijing 100050, China.
Chen
Xiaoping
X
Piao
Jianhua
J
Han
Chi
C
D000818:Animals; D000831:Animals, Newborn; D005260:Female; D008297:Male; D012275:Oryza / Q000235:genetics* / Q000633:toxicity; D018532:Pisum sativum / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000633:toxicity*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013723:Teratogens / Q000633:toxicity*; D014361:Trypsin Inhibitors; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
D010940:Plant Proteins; D013723:Teratogens; D014361:Trypsin Inhibitors; D000516:alpha-Amylases