Identification of GRP 78 (BiP) as a liver cell expressed receptor element for dengue virus serotype 2.
This study sought to identify receptor elements for dengue virus serotype 2 on human liver cells (HepG2) using the viral overlay protein binding assay (VOPBA) technique and Mass Spectrometry fingerprinting. A single major and several minor virus binding bands were observed, and mass spectrometry identified a candidate binding protein for the major binding band as GRP 78 (BiP). GRP78 expression on the cell surface was confirmed, and antibodies directed against both the N and C-terminus of GRP 78 (BiP) altered both the binding of the virus to the cell surface as well as the infectivity profile of HepG2 cells in response to dengue serotype 2 infection. GRP 78 (BiP), which has previously been identified as a co-receptor protein for coxsackievirus A9, is the first non-Fc receptor protein identified for the dengue virus, although GRP78 probably functions as part of a receptor complex.
Jindadamrongwech
S
S
Molecular Pathology Laboratory, Institute of Molecular Biology and Genetics, Mahidol University, Nakorn Pathom, Thailand.
Thepparit
C
C
Smith
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DR
D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism*; D002460:Cell Line; D003716:Dengue Virus / Q000254:growth & development*; D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; D006801:Humans; D007118:Immunoassay; D008099:Liver / Q000378:metabolism / Q000821:virology*; D013058:Mass Spectrometry; D018832:Molecular Chaperones; D010449:Peptide Mapping; D011991:Receptors, Virus / Q000032:analysis / Q000378:metabolism*; D010948:Viral Plaque Assay
D002352:Carrier Proteins; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D011991:Receptors, Virus; C111476:dengue virus receptor