Assessment of molecularly imprinted sol-gel materials for selective room temperature phosphorescence recognition of nafcillin.
Sol-gel imprinted materials were prepared against nafcillin, a semisynthetic beta-lactamic antibiotic employed in the treatment of serious infections caused by penicillinase-producing staphylococci. Two approaches were addressed for preparation of the imprinted materials and the controls: as conventional monoliths, which were ground and sieved to a desired particle size for rebinding analysis, and as films on supporting glass slides. The specific binding sites that are created during the imprinting process are analyzed via selective room temperature phosphorescence (RTP) (sol-gel films) measurements as well as via competitive room temperature phosphorescence ligand assay. Results demonstrated the importance of the physical configuration of the imprinted material for minimizing non-specific binding. The close similarities between the structures of different beta-lactamic antibiotics made it possible to interpret the roles of the template structure on specific molecular recognition. In this article, we introduce the use of room temperature phosphorescence as selective transduction method for the template. The imprinted sol-gel films displayed enhanced specific binding characteristics respect to the monolithic sol-gel and can be envisaged for the use as recognition matrices for the screening and rapid selection of antibiotics from a combinatorial library or for the rapid control of nafcillin in biological samples (e.g. milk, serum, urine).
Fernández-González
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Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, C/ Julian Claveria 8, Oviedo 33006, Spain.
Badía Laíño
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Diaz-García
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ME
Guardia
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Viale
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D005782:Gels; D008163:Luminescent Measurements; D009254:Nafcillin / Q000032:analysis*; D013696:Temperature
D005782:Gels; D009254:Nafcillin