title: The efficient prevascularization induced by fibroblast growth factor 2 with a collagen-coated device improves the cell survival of a bioartificial pancreas.
abstract: OBJECTIVE
  The subcutaneous transplantation of a bioartificial pancreas is a very attractive cure for diabetes mellitus. We recently developed a new immunoisolatory device that has the ability to induce neovascularization for subcutaneous transplantation. We applied the newly developed device to subcutaneous transplantation of a bioartificial pancreas.
  
  
  METHODS
  We investigated the prevascularization-inducing activity of the device in diabetic rats by histologic analysis and evaluated the permeability of the device to insulin and BSA. We also evaluated the survival of cells enclosed in a bioartificial pancreas, which was composed of the device, from the viewpoint of the effects of prevascularization by semiquantitative RT-PCR.
  
  
  RESULTS
  The devices induced prevascularization more efficiently than fibroblast growth factor 2 impregnated in gelatin microspheres alone did and had more useful permeability than a noncollagen-coated device. Significantly higher expression of insulin mRNA was detected in the RT-PCR amplicons from cells retrieved from the bioartificial pancreas transplanted at the prevascularization-induced site as compared with at a nonprevascularization-induced site.
  
  
  CONCLUSIONS
  We demonstrated that our newly developed device has a superior ability to induce prevascularization in diabetic rats, and the prevascularization improves the initial cell survival of the implanted cells following transplantation.
authors.affiliation: Department of Organ Reconstruction, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Sakurai
authors.forename: Akira
authors.initials: A
authors.lastname: Satake
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Sumi
authors.forename: Kazutomo
authors.initials: K
authors.lastname: Inoue
authors.forename: Natsuki
authors.initials: N
authors.lastname: Nagata
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
authors.forename: Junji
authors.initials: J
authors.lastname: Miyakoshi
mesh_terms: D000818:Animals; D021522:Bioartificial Organs; D002460:Cell Line; D002463:Cell Membrane Permeability; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen; D003131:Combined Modality Therapy; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy / Q000473:pathology / Q000601:surgery*; D016222:Fibroblast Growth Factor 2 / Q000627:therapeutic use*; D006085:Graft Survival / Q000187:drug effects; D008297:Male; D018919:Neovascularization, Physiologic; D016035:Pancreas Transplantation; D019397:Pancreas, Artificial; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D016222:Fibroblast Growth Factor 2; D003094:Collagen