Evaluation of analyte stability and method ruggedness in the determination of streptomycin residues in honey by liquid chromatography with post-column derivatization.
This study demonstrated that streptomycin in honey is quite stable, and the results showed no obvious differences for 3 samples containing incurred analyte during continuous testing for 4 months. Fifteen laboratories evaluated method performance at 4 fortification levels ranging from 0.010 to 0.100 mg/kg; the recoveries ranged from 73.7 to 78.5%, the reproducibility relative standard deviations ranged from 5.76 to 15.85%, and the repeatability relative standard deviations ranged from 1.64 to 3.80%. In 1999-2002, the method was used to determine streptomycin residues in 5106 lots of honey samples from >20 provinces all over China. All of the honey samples were found to be in conformity with the requirements of customs clearance for exports to Europe, the United States, and Japan. The continuous 4-year quality analysis also found that C18 solid-phase extraction cartridges should be standardized to ensure that the analytical results are accurate when different lots of cartridges are used.
Pang
Guo-Fang
GF
Qinhuangdao Entry-Exit Inspection and Quarantine Bureau, 39 Haibin Rd, Qinhuangdao, Hebei, PC 066002, People's Republic of China. gfpang@heinfo.net
Zhang
Jin-Jie
JJ
Cao
Yan-Zhong
YZ
Fan
Chun-Lin
CL
Lin
Xue-Min
XM
Li
Zeng-Yin
ZY
Jia
Guo-Qun
GQ
D000900:Anti-Bacterial Agents / Q000032:analysis*; D002021:Buffers; D002411:Cation Exchange Resins; D002853:Chromatography, Liquid; D004350:Drug Residues / Q000032:analysis*; D006722:Honey / Q000032:analysis*; D007202:Indicators and Reagents; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012997:Solvents; D013307:Streptomycin / Q000032:analysis*
D000900:Anti-Bacterial Agents; D002021:Buffers; D002411:Cation Exchange Resins; D007202:Indicators and Reagents; D012997:Solvents; D013307:Streptomycin