[High-performance capillary electrophoresis for determination of urinary citrate and oxalate].
OBJECTIVE
To develop a method for determining urinary citrate and oxalate using high-performance capillary electrophoresis.


METHODS
Capillary electrophoresis with indirect ultraviolet (UV) detection was performed for determining urinary citrate and oxalate. Sodium hromate was used as the UV-absorbing background electrolyte.


RESULTS
Citrate and oxalate had a migration time of 3.76 min and 3.14 min respectively. The calibration curves were linear within a limit of about 2 microg/ml for citrate and about 1 microg/ml for oxalate. The recovery rates of citrate and oxalate were 97.53% and 99.11% respectively.


CONCLUSIONS
This method is simple, accurate and at low cost for determining citrate and oxalate in the urine.
Luo
Qi-zhi
QZ
Central Laboratory, First Military Medical University, Guangzhou 510515, China.
Dai
Kai-jin
KJ
Ma
An-de
AD
Zhou
Jun-ling
JL
D019343:Citric Acid / Q000652:urine*; D019075:Electrophoresis, Capillary; D006801:Humans; D010070:Oxalates / Q000652:urine*
D010070:Oxalates; D019343:Citric Acid