Creation of reversed phase high-performance liquid chromatographic technique to assay platelet-activating factor.
OBJECTIVE
To establish a new assay for platelet-activating factor (PAF), to compare it with bio-assay; and to discuss its significance in some elderly people diseases such as cerebral infarction and coronary heart disease.


METHODS
To measure PAF levels in 100 controls, 23 elderly patients with cerebral infarction and 65 cases with coronary heart disease by reversed phase high-performance liquid chromatographic technique (rHPLC).


RESULTS
rHPLC is more convenient, sensitive, specific, and less confusing, compared with bio-assay. The level of plasma PAF in patients with cerebral infarction was higher than that in the controls (P<0.01), and in patients with coronary heart disease.


CONCLUSIONS
Detection of PAF with rHPLC is more reliable and more accurate. The new assay has important significance in PAF research.
Yang
Yun-mei
YM
First Affiliated Hospital, College of Medical, Zhejiang University, Hangzhou 310003, China.
Cao
Hong-cui
HC
Xu
Zhe-rong
ZR
Chen
Xiao-ming
XM
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D002544:Cerebral Infarction / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003327:Coronary Disease / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010972:Platelet Activating Factor / Q000032:analysis* / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D010972:Platelet Activating Factor