title: A new protocol to measure the effects of toxicants on daphnid-algae interactions.
abstract: Inhibition of zooplankton grazing by toxicants present at sublethal concentrations in freshwater ecosystems can lead to uncontrolled algal growth and consequently exacerbate eutrophication problems. Short-term measurements of cladoceran grazing inhibition have been reported for some toxicants, but these studies do not mimic the actual interactions between microalgae, daphnids and toxicants, since algal growth is not allowed. On the opposite, algal blooms in complex microcosm or mesocosm assays have been interpreted as consequences on zooplankton, but effects on grazing, survival, growth or reproduction could not be easily discriminated. In this study, a simple assay with daphnids and microalgae is proposed to measure effects on grazing in dynamic conditions (algal growth over 6 days), and applied to copper and lindane. In the same time, direct effects on algal growth can be shown and taken into account. Results are compared with daphnid response measured with different endpoints (immobilization test and static grazing assay). For both toxicants, effects at sublethal concentrations were demonstrated. Copper impaired daphnid grazing at 10 microg/l (60% inhibition) in the 48 h-static test and 15 microg/l (40% inhibition) in the 6 day-dynamic test, whereas 48 h-EC50 for daphnid mobility was 47 microg/l. The EC50s for lindane were 50 microg/l for daphnid grazing (48 h-static and 6 day-dynamic tests) and 383 microg/l for the immobilization test (48 h).
authors.affiliation: Ecole Nationale des Travaux Publics de l'Etat, Laboratoire des Sciences de l'Environnement, Rue Maurice Audin, 69518 Vaulx en Velin Cedex, France. bernard.clement@entpe.fr
authors.forename: Bernard
authors.initials: B
authors.lastname: Clément
authors.forename: Soumia
authors.initials: S
authors.lastname: Zaid
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D003300:Copper / Q000633:toxicity; D003621:Daphnia / Q000187:drug effects* / Q000502:physiology; D017753:Ecosystem; D056890:Eukaryota / Q000187:drug effects*; D005247:Feeding Behavior / Q000187:drug effects* / Q000502:physiology; D005618:Fresh Water; D015386:Hazardous Substances / Q000633:toxicity*; D001556:Hexachlorocyclohexane / Q000633:toxicity; D018675:Toxicity Tests
chemical_list: D015386:Hazardous Substances; D001556:Hexachlorocyclohexane; D003300:Copper