Rapid enzymatic transglycosylation and oligosaccharide synthesis in a microchip reactor.
Glycosidase-promoted hydrolysis was performed in a microreaction channel. The result was compared with the reaction in a micro-test tube. Transgalactosylation on p-nitrophenyl-2-acetamide-2-deoxy-beta-D-glucopyranoside was also examined in a microreaction channel, because transglycosylation is a useful method for oligosaccharide synthesis. We examined both the forward reaction, i.e., hydrolysis, and the reverse reaction, i.e., transglycosylation, in the microreaction channel. The results showed that both hydrolysis and transglycosylation were enhanced in the microreaction channel compared with the batch reaction.
Kanno
Ken-ichi
K
Department of Bio-Environmental Chemistry, Kyushu School of Engineering, Kinki University, Kayanomori 11-6, Iizuka-shi, Fukuoka, 820-8555, Japan.
Maeda
Hideaki
H
Izumo
Showta
S
Ikuno
Masakazu
M
Takeshita
Kazuhito
K
Tashiro
Asuka
A
Fujii
Masayuki
M
D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000737:chemistry; D006031:Glycosylation; D008904:Miniaturization; D009844:Oligosaccharides / Q000138:chemical synthesis*; D001616:beta-Galactosidase / Q000737:chemistry*
D009844:Oligosaccharides; C014124:4-nitrophenyl-N-acetyl-2-deoxyglucopyranoside; D001616:beta-Galactosidase; D000117:Acetylglucosamine