Effects of maribavir and selected indolocarbazoles on Epstein-Barr virus protein kinase BGLF4 and on viral lytic replication.
The human cytomegalovirus (HCMV) homolog of the Epstein-Barr virus (EBV) protein kinase (PK), UL97, is inhibited by maribavir (1263W94) and selected indolocarbazoles. Here we show that only one of these indolocarbazoles (K252a), but not maribavir, inhibits autophosphorylation of the EBV PK, BGLF4. However, maribavir and another indolocarbazole, NGIC-I, do inhibit EBV DNA synthesis, suggesting that although these last compounds inhibit both HCMV and EBV, they seem to operate through differ-ent pathways.
Gershburg
Edward
E
Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
Hong
Ke
K
Pagano
Joseph S
JS
D001562:Benzimidazoles / Q000494:pharmacology*; D002227:Carbazoles / Q000494:pharmacology*; D002384:Catalysis; D002460:Cell Line; D003587:Cytomegalovirus / Q000187:drug effects; D004279:DNA, Viral / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004854:Herpesvirus 4, Human / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors* / Q000235:genetics; D012263:Ribonucleosides / Q000494:pharmacology*; D014764:Viral Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D014779:Virus Replication / Q000187:drug effects*; D004004:Dichlororibofuranosylbenzimidazole / Q000031:analogs & derivatives
D001562:Benzimidazoles; D002227:Carbazoles; D004279:DNA, Viral; D004791:Enzyme Inhibitors; D019384:Nucleic Acid Synthesis Inhibitors; D017346:Protein Serine-Threonine Kinases; D012263:Ribonucleosides; D014764:Viral Proteins; C406063:BGLF4 protein, Epstein-Barr virus; C400401:maribavir; D004004:Dichlororibofuranosylbenzimidazole