title: Alpha-amylase inhibitors from Ficus sp. seeds and their activities towards coleoptera insect pests.
abstract: Alpha-amylase Inhibitors were isolated from Ficus sp. (Gameleira) seeds by acetone fractionation and Sephadex G-50. Two inhibitors (alpha-PPAI and alpha-ZSAI) were tested against alpha-amylases from coleopteran larvae. alpha-PPAI was active to alpha-amylases of Callosobruchus maculatus (52%) and Zabrotes subfasciatus (53%). alpha-ZSAI was strongly active to Z. subfasciatus (100%) of and Mimosestes mimosae (98%). The alpha-ZSAI is a glycoprotein of approximately 50 kDa with an IC50 value of 0.074 microg microl(-1).
authors.affiliation: Departamento de Bioquimica, Centro de Biociências, Universidade Federal do Rio Grande do Norte, 59072-970, Natal/RN, Brazil.
authors.forename: Ingrid W L
authors.initials: IW
authors.lastname: Bezerra
authors.forename: Fabiano M
authors.initials: FM
authors.lastname: Teixeira
authors.forename: Adeliana S
authors.initials: AS
authors.lastname: Oliveira
authors.forename: Carina L
authors.initials: CL
authors.lastname: Araújo
authors.forename: Edda L
authors.initials: EL
authors.lastname: Leite
authors.forename: Karla C S
authors.initials: KC
authors.lastname: Queiroz
authors.forename: Maurício P
authors.initials: MP
authors.lastname: Sales
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000187:drug effects* / Q000201:enzymology; D030681:Ficus / Q000737:chemistry*; D007814:Larva / Q000187:drug effects / Q000201:enzymology; D010571:Pest Control; D010940:Plant Proteins / Q000737:chemistry* / Q000633:toxicity; D012639:Seeds / Q000737:chemistry; D014361:Trypsin Inhibitors; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
chemical_list: D010940:Plant Proteins; D014361:Trypsin Inhibitors; D000516:alpha-Amylases