Novel, rapid, and inexpensive cell-based quantification of antimalarial drug efficacy.
We report on the development of a new SYBR Green I-based plate assay for analyzing the activities of antimalarial drugs against intraerythrocytic Plasmodium falciparum. This assay is considerably faster, less labor-intensive, and less expensive than conventional radiotracer (e.g., [3H]hypoxanthine and [3H]ethanolamine)-based assays or P. falciparum lactate dehydrogenase activity-based assays. The assay significantly improves the pace at which antimalarial drug discovery efforts may proceed.
Bennett
Tyler N
TN
Department of Chemistry, Lombardi Cancer Center, Georgetown University, Washington DC 20057, USA.
Paguio
Michelle
M
Gligorijevic
Bojana
B
Seudieu
Clement
C
Kosar
Andrew D
AD
Davidson
Eugene
E
Roepe
Paul D
PD
D000818:Animals; D000962:Antimalarials / Q000191:economics / Q000494:pharmacology*; D052160:Benzothiazoles; D002738:Chloroquine / Q000494:pharmacology; D003959:Diamines; D004353:Drug Evaluation, Preclinical / Q000191:economics / Q000379:methods*; D004351:Drug Resistance; D004912:Erythrocytes / Q000469:parasitology; D005456:Fluorescent Dyes; D006801:Humans; D018613:Microscopy, Confocal; D009930:Organic Chemicals; D010963:Plasmodium falciparum / Q000187:drug effects; D011804:Quinolines
D000962:Antimalarials; D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; D002738:Chloroquine