Sequence analysis of 0.5 Mb of the rabbit germline immunoglobulin heavy chain locus.
A bacterial artificial chromosome (BAC) library was created using partially digested rabbit chromosomal DNA. Four BAC clones spanning about 0.5 Mb of the rabbit immunoglobulin (Ig) heavy chain locus were isolated and sequenced. Three of the BAC clones were partially overlapping. Thirty-four V elements, 11 D elements, DQ52, six J elements and the coding regions of Cmicro, Cgamma, C and four Calpha genes were identified and characterized. Additionally, the sequence of a fosmid clone spanning Calpha13 and 30 kb 3'enhancer region was determined. The organization of the locus and the potential function of newly identified functional and structural elements are discussed.
Ros
Francesca
F
University of Munich, Institut für Molekulare Tierzucht und Biotechnologie, Feodor-Lynen-Str. 25, 81377 München, Germany.
Puels
Juergen
J
Reichenberger
Nicole
N
van Schooten
Wim
W
Buelow
Roland
R
Platzer
Josef
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D004247:DNA / Q000737:chemistry / Q000235:genetics; D015698:Genomic Library; D018095:Germ-Line Mutation; D007073:Immunoglobulin E / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D011817:Rabbits / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D007143:Immunoglobulin Heavy Chains; D007073:Immunoglobulin E; D004247:DNA