[Anti-oncogene p53-induced expression of secreted frizzled-related protein, a Wnt signaling inhibitor].
OBJECTIVE
To investigate the effects of anti-oncogene p53 on the expression of secreted frizzled-related protein (sFRP), a Wnt pathway inhibitor.


METHODS
Human hepatocarcinoma Hep3B cells were transfected with a replication-defective adenovirus encoding p53 (Adp53), and Adp53 transgene expression was measured by fluorescence-activated cell sorting (FACS) and sFRP mRNA expression detected by reverse transcriptional (RT)-PCR.


RESULTS
sFRP mRNA expression in Hep3B cells was potentiated 20 h after the transfection with Adp53, reaching the peak level at 32 h. Dose-effect studies revealed that Adp53 at the doses of 0.5, 5 and 50 pfu/cell could promote the expression of sFRP mRNA, with the highest expression occurred with the dose of 5 pfu/cell.


CONCLUSIONS
Ectogenetic p53 promotes mRNA expression of Wnt signaling pathway inhibitor sFRP in Hep3B cells.
La
Lei
L
Institute of Pharmaceutic Science, First Military Medical University, Guangzhou 510515, China. LaLei88@163.com
Rao
Jin-jun
JJ
Wu
Shu-guang
SG
D000818:Animals; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D008113:Liver Neoplasms / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors*; D012333:RNA, Messenger / Q000032:analysis; D015398:Signal Transduction; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000502:physiology*; D051153:Wnt Proteins; D000094742:Secreted Frizzled-Related Proteins
D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D051153:Wnt Proteins; C117636:SFRP1 protein, human; D000094742:Secreted Frizzled-Related Proteins; C485539:Sfrp1 protein, mouse