Transcriptome variations in human CaCo-2 cells: a model for enterocyte differentiation and its link to iron absorption.
Complete clinical expression of the HFE1 hemochromatosis is very likely modulated by genes linked to duodenal iron absorption, whose level is conditioned by unknown processes taking place during enterocyte differentiation. We carried out a transcriptomic study on CaCo-2 cells used as a model of enterocyte differentiation in vitro. Of the 720 genes on the microarrays, 80, 50, and 56 were significantly down-regulated up-regulated, and invariant during differentiation. With regard to iron metabolism, we showed that HEPH, SLC11A2, SLC11A3, and TF are significantly up-regulated, while ATP7B and SLC39A1 (and SFT) are down-regulated and ACO1, dCYTb, FECH, and FTH1 show constant expression. Ontological annotations highlight the decrease in the expression of cell cycle and DNA metabolism associated genes as well as transcription, protein metabolism, signal transduction, and nucleocytoplasmic transport associated genes, whereas there are increases in the expression of genes linked to cell adhesion, lipid and xenobiotic metabolism, iron transport and homeostasis, and immune response.
Bédrine-Ferran
Hélène
H
UMR-6061 CNRS, IFR 97, Faculté de Médecine, CS 34317-35043 Rennes, France.
Le Meur
Nolwenn
N
Gicquel
Isabelle
I
Le Cunff
Martine
M
Soriano
Nicolas
N
Guisle
Isabelle
I
Mottier
Stéphanie
S
Monnier
Annabelle
A
Teusan
Raluca
R
Fergelot
Patricia
P
Le Gall
Jean-Yves
JY
Léger
Jean
J
Mosser
Jean
J
D018938:Caco-2 Cells; D002454:Cell Differentiation; D020895:Enterocytes / Q000166:cytology* / Q000378:metabolism*; D020869:Gene Expression Profiling; D023281:Genomics; D006801:Humans; D007501:Iron / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000235:genetics*
D012333:RNA, Messenger; D007501:Iron