Glycosyltransferase activity can be selectively modulated by chemical modifications of acceptor substrates.
A range of N-acetyllactosamine derivatives, which are modified by a wide range of functionalities at C-2(') and C-6, have been synthesised and the kinetic parameters of transfer catalysed by recombinant alpha-2,6-sialyltransferase and alpha-1,3-fucoyltransferase VI determined. Several of the chemical modifications led to selective modulate the activity the enzymes and offer promising lead compounds for the development of oligosaccharide primers for selective metabolic inhibition of oligosaccharide biosynthesis.
Galan
M Carmen
MC
Complex Carbohydrate Research Center, University of Georgia, 220 Riverbend Road, Athens, GA 30602, USA.
Dodson
Christopher S
CS
Venot
Andre P
AP
Boons
Geert-Jan
GJ
D000606:Amino Sugars / Q000737:chemistry / Q000494:pharmacology*; D002240:Carbohydrate Sequence; D002384:Catalysis; D016695:Glycosyltransferases / Q000378:metabolism*; D007700:Kinetics; D013379:Substrate Specificity
D000606:Amino Sugars; C000458:N-acetyllactosamine; D016695:Glycosyltransferases