High genetic stability of TM1 and TM2 strains of subtype B feline immunodeficiency virus in long-term infection.
To know the genetic changes of feline immunodeficiency virus (FIV) in long-term infection in cats, we inoculated three specific pathogen-free cats with FIV isolates and determined a partial env sequence covering the V3-V5 region. In 2 cats infected with subtype B strains TM1 and TM2, only one amino acid change in region V3 was observed at 9 years post infection (y.p.i.), and no nucleotide substitutions were observed between 9 and 10 y.p.i., indicating that these strains are genetically stable. On the other hand, in a cat infected with subtype A strain Petaluma at 8.7 y.p.i., 3 nucleotide insertions (one amino acid insertion) in region V5, and 1 synonymous nucleotide substitution and 2 non-synonymous nucleotide substitutions in region V5, were observed.
Ikeda
Yasuhiro
Y
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
Miyazawa
Takayuki
T
Nishimura
Yorihiro
Y
Nakamura
Kazuya
K
Tohya
Yukinobu
Y
Mikami
Takeshi
T
D000595:Amino Acid Sequence; D000818:Animals; D002371:Cat Diseases / Q000235:genetics* / Q000821:virology; D002415:Cats; D015752:Genes, env / Q000235:genetics*; D016679:Genome, Viral; D042822:Genomic Instability; D016089:Immunodeficiency Virus, Feline / Q000235:genetics*; D016180:Lentivirus Infections / Q000235:genetics / Q000662:veterinary*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D017422:Sequence Analysis, DNA