Phylogenetic characterization of a corrosive consortium isolated from a sour gas pipeline.
Biocorrosion is a common problem in oil and gas industry facilities. Characterization of the microbial populations responsible for biocorrosion and the interactions between different microorganisms with metallic surfaces is required in order to implement efficient monitoring and control strategies. Denaturing gradient gel electrophoresis (DGGE) analysis was used to separate PCR products and sequence analysis revealed the bacterial composition of a consortium obtained from a sour gas pipeline in the Gulf of Mexico. Only one species of sulfate-reducing bacteria (SRB) was detected in this consortium. The rest of the population consisted of enteric bacteria with different characteristics and metabolic capabilities potentially related to biocorrosion. Therefore, several types of bacteria may be involved in biocorrosion arising from natural biofilms that develop in industrial facilities. The low abundance of the detected SRB was evidenced by environmental scanning electron microscopy (ESEM). In addition, the localized corrosion of pipeline steel in the presence of the consortium was clearly observed by ESEM after removing the adhered bacteria.
Jan-Roblero
J
J
Programa de Investigación Desarrollo en Biotecnología del Petróleo, Instituto Mexicano del Petróleo, Eje Central # 152, Col. San Bartolo Atepehuacan, 07730 Mexico, DF, Mexico.
Romero
J M
JM
Amaya
M
M
Le Borgne
S
S
D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D018441:Biofilms / Q000145:classification*; D002617:Chemical Industry / Q000592:standards; D003343:Corrosion; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006089:Gram-Negative Anaerobic Bacteria / Q000235:genetics / Q000302:isolation & purification; D008670:Metals; D008800:Mexico; D008854:Microscopy, Electron / Q000379:methods; D008969:Molecular Sequence Data; D010578:Petroleum / Q000382:microbiology* / Q000592:standards / Q000600:supply & distribution; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D008670:Metals; D010578:Petroleum; D012336:RNA, Ribosomal, 16S