Growth inhibition and mutagenesis induced in Escherichia coli by dihydropyrazines with DNA strand-cleaving activity.
Dihydropyrazine (DHP) causes DNA strand breaks in vitro. We evaluated the cytotoxic and genotoxic potential of DHP in Escherichia coli. DHP exposure dose-dependently caused inhibition of cell growth in the wild-type strain, death in recA and uvrB, and an increase in mutation frequency in uvrB. These findings indicate that DHP causes DNA strand breaks in vivo.
Takechi
Shinji
S
Department of Biochemistry, Miyazaki Medical College, Kihara, Kiyotake, Miyazaki 889-1692, Japan. stakechi@post.med.miyazaki-u.ac.jp
Yamaguchi
Tadatoshi
T
Nomura
Hajime
H
Minematsu
Toshio
T
Nakayama
Tatsuo
T
D004249:DNA Damage; D004269:DNA, Bacterial / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D016296:Mutagenesis; D011719:Pyrazines / Q000633:toxicity*
D004269:DNA, Bacterial; D011719:Pyrazines