title: Immunohistochemical analysis of adhesion molecule expression on muscle biopsy specimens from patients with juvenile dermatomyositis.
abstract: OBJECTIVE
  To assess expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) on muscle biopsy specimens from patients with untreated juvenile dermatomyositis (JDM).
  
  
  METHODS
  Histochemical and immunohistochemical tests for ICAM-1 and VCAM-1 were performed on serial frozen sections from 27 JDM muscle biopsy specimens. ICAM-1 and VCAM-1 expression was analyzed on capillaries, perimysial and endomysial large vessels, and muscle fibers. Expression was assessed and graded semiquantitatively.
  
  
  RESULTS
  Increased ICAM-1 expression was observed on capillaries and perimysial large vessels on semiquantitative analysis, and was statistically more evident than on endomysial large vessels. In all cases, only a few muscle vessels showed expression of VCAM-1. Expression of ICAM-1 and VCAM-1 was observed on few muscle fibers.
  
  
  CONCLUSIONS
  The observation of ICAM-1 expression on muscle vessels, mainly on capillaries of patients with untreated JDM compared to controls, and VCAM-1 expression to a lesser extent, mostly on muscle vessels surrounded by inflammatory infiltrate, supports the participation of these adhesion molecules in the pathologic mechanism of vascular injury in JDM.
authors.affiliation: Department of Pediatrics, University of São Paulo Medical School, São Paulo, Brazil. adrianasallum@ig.com.br
authors.forename: Adriana M E
authors.initials: AM
authors.lastname: Sallum
authors.forename: Suely K N
authors.initials: SK
authors.lastname: Marie
authors.forename: Alda
authors.initials: A
authors.lastname: Wakamatsu
authors.forename: Silvana
authors.initials: S
authors.lastname: Sachetti
authors.forename: Maria A A G
authors.initials: MA
authors.lastname: Vianna
authors.forename: Clóvis A A
authors.initials: CA
authors.lastname: Silva
authors.forename: Maria H B
authors.initials: MH
authors.lastname: Kiss
mesh_terms: D000293:Adolescent; D001706:Biopsy; D001808:Blood Vessels / Q000378:metabolism / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D003882:Dermatomyositis / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*
chemical_list: D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1