In vitro assays for kinetoplastid U insertion-deletion editing and associated activities.
This chapter describes biochemical assays that have been used in analyzing RNA editing in kinetoplastid mitochondria and to characterize the general mechanism of editing by the editosome. Studies using these assays have shown that the characteristics of each activity contribute to editing site selection, U addition and removal, and RNA ligation resulting in accurately edited mRNAs.
Stuart
Kenneth
K
Seattle Biomedical Research Institute, and Department of Pathobiology, University of Washington, Seattle, USA.
Salavati
Reza
R
Igo
Robert P
RP
Lewis Ernst
Nancy
N
Palazzo
Setareh S
SS
Wang
Bingbing
B
D000818:Animals; D001483:Base Sequence; D016830:Kinetoplastida / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction / Q000379:methods; D017393:RNA Editing / Q000235:genetics*; D016337:RNA, Catalytic / Q000235:genetics; D016053:RNA, Protozoan / Q000235:genetics; D017384:Sequence Deletion; D014498:Uracil
D016337:RNA, Catalytic; D016053:RNA, Protozoan; C427204:hammerhead ribozyme; D014498:Uracil