[Enzyme activity of nucleocapsid proteins of the curly potato dwarf virus].
The individual nucleocapsid proteins of phytorhabdovirus of curly potato dwarf were isolated: N-protein (m. w. 56 kDa), NS-protein (m. w. 49 kDa), L-protein (m. w. 128 kDa). It was establish that L and NS proteins displayed enzyme activity in the replication, phosphorylation and adenylation systems in vitro. Major N protein (m. w. 56 kDa) did not show the enzyme activity in these systems. The revelation of RNA-polymerase, poly(A)-polymerase and proteinkinase activities of nucleocapsid proteins are characteristic of the viruses belonging to the Rhabdoviridae family.
Parkhomenko
N I
NI
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv.
Didenko
L F
LF
Maksimenko
L A
LA
Diachenko
N S
NS
D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism; D019590:Nucleocapsid Proteins / Q000302:isolation & purification / Q000378:metabolism*; D010766:Phosphorylation; D011061:Poly A / Q000378:metabolism; D011062:Polynucleotide Adenylyltransferase / Q000378:metabolism; D012209:Rhabdoviridae / Q000201:enzymology*
D019590:Nucleocapsid Proteins; D011061:Poly A; D011062:Polynucleotide Adenylyltransferase; D012321:DNA-Directed RNA Polymerases; D010450:Endopeptidases