title: Characterization of a new HpaI centromeric satellite DNA in Salmo salar.
abstract: A highly repeated HpaI DNA family was revealed in Atlantic salmon (Salmo salar) and analyzed by Southern blotting and fluorescence in situ hybridization (FISH). In this report, we describe the nucleotide sequence, genomic structure and chromosomal localization of this HpaI repeat. This novel satellite appeared tandemly arrayed and located at centromeric areas of three acrocentric chromosome pairs as evidenced by FISH. The sequence was characterized by a high AT content (63%), a short consensus motif (A/T)(G/C)AAA(T/C) similar to other centromeric satellites motifs, and by short AT enriched stretches. The presence of this sequence in other salmonid species was also tested by Southern blot hybridization and used to analyze its evolution within this group.
authors.affiliation: Departamento de Genética, Facultad de Biología, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain. avinas@lugo.usc.es
authors.forename: Ana
authors.initials: A
authors.lastname: Viñas
authors.forename: María
authors.initials: M
authors.lastname: Abuín
authors.forename: Belén G
authors.initials: BG
authors.lastname: Pardo
authors.forename: Paulino
authors.initials: P
authors.lastname: Martínez
authors.forename: Laura
authors.initials: L
authors.lastname: Sánchez
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002503:Centromere; D004276:DNA, Satellite / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D020317:Salmo salar / Q000235:genetics*
chemical_list: D004276:DNA, Satellite