Identification of Chromobacterium violaceum genes with potential biotechnological application in environmental detoxification.
Chromobacterium violaceum is a Gram-negative bacterium found in a wide variety of tropical and subtropical ecosystems. The complete genome sequence of C. violaceum ATCC 12472 is now available, and it has considerable biotechnological potential for various applications, such as environmental detoxification, as well as medical and agricultural use. We examined the biotechnological potential of C. violaceum for environmental detoxification. Three operons, comprising the ars operon, involved in arsenic resistance, the cyn operon, involved in cyanate detoxification, and the hcn operon, encoding a cyanase, responsible for biogenic production of cyanide, as well as an open reading frame, encoding an acid dehalogenase, were analyzed in detail. Probable catalytic mechanisms for the enzymes were determined, based on amino acid sequence comparisons and on published structural information for these types of proteins.
Carepo
Marta S P
MS
Departamento de Genética/Laboratório de Polimorfismo de DNA, Universidade Federal do Pará, Campus Universitário do Guamá, Rua Augusto Correa, 01, 66075-970 Belém, PA, Brasil.
Azevedo
Juliana S Nina de
JS
Porto
Jorge I R
JI
Bentes-Sousa
Alexandra R
AR
Batista
Jacqueline da Silva
Jda S
Silva
Artur L C da
AL
Schneider
Maria P C
MP
D001151:Arsenic / Q000378:metabolism / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001673:Biodegradation, Environmental; D001709:Biotechnology; D002861:Chromobacterium / Q000235:genetics* / Q000378:metabolism; D003486:Cyanides / Q000378:metabolism; D024881:Drug Resistance, Bacterial / Q000235:genetics; D006867:Hydrolases / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D009876:Operon / Q000235:genetics
D001426:Bacterial Proteins; D003486:Cyanides; D006867:Hydrolases; C034507:2-haloacid dehalogenase; D001151:Arsenic