Natural polymorphism of cytomegalovirus DNA polymerase lies in two nonconserved regions located between domains delta-C and II and between domains III and I.
We described the natural polymorphism of cytomegalovirus DNA polymerase in 42 unrelated isolates susceptible to ganciclovir, foscarnet, and cidofovir. All variations, including an eight-amino-acid deletion, were located between domains delta-C and II and between domains III and I, suggesting that these specific residues are not involved in enzymatic functions.
Fillet
Anne-Marie
AM
Virology Laboratories, Hospital AP-HP Pitié-Salpêtrière and University Paris VI, Paris, France. anne-marie.fillet@psl.ap-hop-paris.fr
Auray
Laetitia
L
Alain
Sophie
S
Gourlain
Karine
K
Imbert
Berthe Marie
BM
Najioullah
Fatiha
F
Champier
Gael
G
Gouarin
Stéphanie
S
Carquin
Jocelyne
J
Houhou
Nadhira
N
Garrigue
Isabelle
I
Ducancelle
Alexandra
A
Thouvenot
Danielle
D
Mazeron
Marie-Christine
MC
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000998:Antiviral Agents / Q000494:pharmacology; D017124:Conserved Sequence; D003587:Cytomegalovirus / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D017353:Gene Deletion; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000998:Antiviral Agents; D004259:DNA-Directed DNA Polymerase