Elimination of zinc-65 from the brain under kainate-induced seizures.
On the basis of the previous evidence that 65Zn concentrations in the brain of EL (epilepsy) mice was affected by induction of seizures, 65Zn movement in the brain was quantitatively evaluated in ddY mice treated with kainate. Six days after intravenous injection of 65ZnCl2, mice were intraperitoneally injected with kainate (10 mg/kg x 6 times in 2 weeks). Myoclonic jerks were observed during treatment with kainate. Twenty days after 65Zn injection, 65Zn distribution in the brain was compared between the kainite-treated and control mice. 65Zn distribution in the brain of the kainate-treated mice was overall lower than in the control mice. 65Zn concentration was significantly decreased in the frontal cortex, hippocampal CA1, thalamus and hypothalamus by treatment with kainate. These results demonstrate that kainate-induced seizures are linked to decreased zinc concentrations in the brain.
Takeda
Atsushi
A
Department of Medical Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52- 1 Yada, Shizuoka 422-8526, Japan. takedaa@u-shizuoka-ken.ac.jp
Hirate
Maki
M
Oku
Naoto
N
D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D007608:Kainic Acid / Q000494:pharmacology; D008297:Male; D008667:Metalloproteins / Q000378:metabolism; D051379:Mice; D009928:Organ Specificity; D011859:Radiography; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism*; D017967:Zinc Compounds / Q000378:metabolism; D015037:Zinc Radioisotopes / Q000378:metabolism*
D002712:Chlorides; D018690:Excitatory Amino Acid Agonists; D008667:Metalloproteins; D017967:Zinc Compounds; D015037:Zinc Radioisotopes; C016837:zinc chloride; D007608:Kainic Acid