title: Development of colonic neoplasia in p53 deficient mice with experimental colitis induced by dextran sulphate sodium.
abstract: BACKGROUND
  Several animal models for human ulcerative colitis (UC) associated neoplasia have been reported. However, most neoplasias developed in these models have morphological and genetic characteristics different from UC associated neoplasia.
  
  
  OBJECTIVE
  To establish a new colitis associated neoplasia model in p53 deficient mice by treatment with dextran sulphate sodium (DSS).
  
  
  METHODS
  DSS colitis was induced in homozygous p53 deficient mice (p53(-/-)-DSS), heterozygous p53 deficient mice (p53(+/-)-DSS) and wild-type mice (p53+/+-DSS) by treatment with 4% DSS. Numbers of developed neoplasias were compared among the experimental groups, and macroscopic and microscopic features of the neoplasias were analysed. Furthermore, K-ras mutation and beta-catenin expression were assessed.
  
  
  RESULTS
  p53(-/-)-DSS mice showed 100% incidence of neoplasias whereas the incidences in p53(+/-)-DSS and p53+/+-DSS mice were 46.2% and 13.3%, respectively. No neoplasias were observed in the control groups. The mean numbers of total neoplasias per mouse were 5.0 (p53(-/-)-DSS), 0.62 (p53(+/-)-DSS), and 0.2 (p53+/+-DSS). The number of neoplasias per mouse in the p53(-/-)-DSS group was significantly higher than that in the other DSS groups. The incidences of superficial type neoplasias were 91.7% in p53(-/-)-DSS mice, 75.0% in p53(+/-)-DSS mice, and 33.3% in p53+/+-DSS mice. The K-ras mutation was not detected in any of the neoplasias tested. Translocation of beta-catenin from the cell membrane to the cytoplasm or nucleus was observed in 19 of 23 (82.6%) neoplasias.
  
  
  CONCLUSIONS
  The p53(-/-)-DSS mice is an excellent animal model of UC associated neoplasia because the morphological features and molecular genetics are similar to those of UC associated neoplasia. Therefore, this model will contribute to the analysis of tumorigenesis related to human UC associated neoplasia and the development of chemopreventive agents.
authors.affiliation: Department of Gastroenterology and Hepatology, Kyoto University Graduate School of Medicine, Kyoto, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Fujii
authors.forename: T
authors.initials: T
authors.lastname: Fujimori
authors.forename: H
authors.initials: H
authors.lastname: Kawamata
authors.forename: J
authors.initials: J
authors.lastname: Takeda
authors.forename: K
authors.initials: K
authors.lastname: Kitajima
authors.forename: F
authors.initials: F
authors.lastname: Omotehara
authors.forename: T
authors.initials: T
authors.lastname: Kaihara
authors.forename: T
authors.initials: T
authors.lastname: Kusaka
authors.forename: K
authors.initials: K
authors.lastname: Ichikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Ohkura
authors.forename: Y
authors.initials: Y
authors.lastname: Ono
authors.forename: J
authors.initials: J
authors.lastname: Imura
authors.forename: S
authors.initials: S
authors.lastname: Yamaoka
authors.forename: C
authors.initials: C
authors.lastname: Sakamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Ueda
authors.forename: T
authors.initials: T
authors.lastname: Chiba
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000150:complications* / Q000473:pathology; D003110:Colonic Neoplasms / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D003598:Cytoskeletal Proteins / Q000378:metabolism; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D016158:Genes, p53; D011905:Genes, ras; D020022:Genetic Predisposition to Disease; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D018345:Mice, Knockout; D009154:Mutation; D015534:Trans-Activators / Q000378:metabolism; D051176:beta Catenin
chemical_list: C495267:CTNNB1 protein, mouse; D003598:Cytoskeletal Proteins; D015534:Trans-Activators; D051176:beta Catenin; D016264:Dextran Sulfate