Evaluating human sperm DNA integrity: relationship between 8-hydroxydeoxyguanosine quantification and the sperm chromatin structure assay.
In our work, we have used 8-hydroxy-deoxyguanosine (8-OH-dG), one of the major oxidative products of sperm DNA, in a population of patients consulting for infertility. We found an inverse relationship between sperm concentration and the log of the ratio of 8-OH-dG to dG (P < 0.01). On the same patients' sperm samples, the sperm chromatin structure assay (SCSA) was performed. An inverse relationship was observed between the DNA fragmentation index and sperm concentration (P < 0.001). There was also a positive relationship between SCSA and log 8-OH-dG/dG. This indicates that DNA fragmentation measured by the SCSA originates in part from oxidative products. In a few patients, antioxidant treatment decreased the DNA fragmentation index below the threshold of 30% that is crucial for subfertility.
Oger
Isabelle
I
Institut Rhonalpin (IRH) Laboratoire Marcel Merieux, 1 Rue Laborde, 69500 Bron, France.
Da Cruz
Christelle
C
Panteix
Gilles
G
Menezo
Yves
Y
D000080242:8-Hydroxy-2'-Deoxyguanosine / Q000031:analogs & derivatives; D002843:Chromatin; D002851:Chromatography, High Pressure Liquid; D053938:DNA Fragmentation / Q000187:drug effects / Q000502:physiology*; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism*; D006801:Humans; D007246:Infertility / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000494:pharmacology; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism
D002843:Chromatin; D017382:Reactive Oxygen Species; D006147:Guanine; C078307:8-oxo-7,8-dihydrodeoxyguanine; D000080242:8-Hydroxy-2'-Deoxyguanosine