title: Moving boundaries in sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
abstract: In sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), moving boundaries I-IV (Table 1) form. They migrate isotachophoretically at displacement rates that increase in the order of I to II to III. Moving boundaries IV and V comprising Pyronin-SDS as a leading constituent are retarded at high gel concentrations in comparison with the isotachophoretically migrating species. Since analytes depending on their net mobilities stack within any of those moving boundaries, previous R(f) values and Ferguson plots may have to be revised.
authors.affiliation: Agricultural Biotechnology Center, Gödöllo, Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Buzás
authors.forename: Andreas
authors.initials: A
authors.lastname: Chrambach
mesh_terms: D002021:Buffers; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*
chemical_list: D002021:Buffers