title: Increased expression of a glutamine transporter SNAT3 is a marker of malignant gliomas.
abstract: Glutamine (Gln) is a growth determinant in neoplastic tissues. We analysed by RT-PCR the expression of mRNAs coding for the human variants of Gln transporters: ASCT2 (system ASC), SNAT1 [ATA1] (system A), SNAT3 [SN1] and SNAT5 [SN2] (system N), in samples of human malignant gliomas WHO grades III/IV (anaplastic astrocytoma and glioblastoma), glioma-derived cell cultures, brain metastases from peripheral organs, and control brain tissue. SNAT3 mRNA showed a 3-5 times stronger expression in gliomas than in metastases or control tissue, and was virtually absent from glioma cultures. Native glioblastoma immunostained positively with anti-SNAT3 antibody. The expression of ASCT2 mRNA, but not SNAT5 or SNAT1 mRNAs, was increased in all neoplastic tissues studied. Hence, increased expression of SNAT3 is a marker of primary malignant gliomas in situ.
authors.affiliation: Department of Neurotoxicology, Medical Research Centre, Polish Academy of Science, Warsaw, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Sidoryk
authors.forename: Ewa
authors.initials: E
authors.lastname: Matyja
authors.forename: Anna
authors.initials: A
authors.lastname: Dybel
authors.forename: Magdalena
authors.initials: M
authors.lastname: Zielinska
authors.forename: Jacek
authors.initials: J
authors.lastname: Bogucki
authors.forename: Dariusz J
authors.initials: DJ
authors.lastname: Jaskólski
authors.forename: Pawel P
authors.initials: PP
authors.lastname: Liberski
authors.forename: Pawel
authors.initials: P
authors.lastname: Kowalczyk
authors.forename: Jan
authors.initials: J
authors.lastname: Albrecht
mesh_terms: D026924:Amino Acid Transport System A / Q000235:genetics; D026962:Amino Acid Transport System ASC / Q000235:genetics; D026921:Amino Acid Transport Systems, Neutral / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D005910:Glioma / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D015778:Minor Histocompatibility Antigens; D011237:Predictive Value of Tests; D012333:RNA, Messenger / Q000378:metabolism; D015203:Reproducibility of Results; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000502:physiology
chemical_list: D026924:Amino Acid Transport System A; D026962:Amino Acid Transport System ASC; D026921:Amino Acid Transport Systems, Neutral; D014408:Biomarkers, Tumor; D015778:Minor Histocompatibility Antigens; D012333:RNA, Messenger; C420506:SLC1A5 protein, human; C445761:SLC38A1 protein, human; C424137:SLC38A5 protein, human; C403222:system N protein 1