Bacterial phospholipid molecular species analysis by ion-pair reversed-phase HPLC/ESI/MS.
This work set out to optimize the detection and separation of several phospholipid molecular species on a reversed-phase column with the use of an electrospray ionization/mass spectrometry-compatible counter-ion. An application of this technique concerned a qualitative and quantitative analysis of bacterial membrane phospholipids extracted from Corynebacterium species strain 8. The phospholipid classes of strain 8 were identified as phosphatidylglycerol, phosphatidylinositol, diphosphatidylglycerol, and a peculiar lipid compound, acyl phosphatidylglycerol. Most of the molecular species structures were elucidated, and regarding phosphatidylglycerol, the fatty acid positions were clearly determined with the calculation of the sn-2/sn-1 intensity ratio of the fatty acyl chain fragments.
Mazzella
Nicolas
N
Laboratoire de Chimie Analytique de l'Environnement, Unité Mixte de Recherche 6171, Institut Fédératif de Recherche Pôle Méditerannéen des Sciences de l'Environement 112, Europôle de l'Arbois, BP 80, 13545 Aix-en-Provence Cedex 4, France.
Molinet
Josiane
J
Syakti
Agung Dhamar
AD
Dodi
Alain
A
Doumenq
Pierre
P
Artaud
Jacques
J
Bertrand
Jean-Claude
JC
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003352:Corynebacterium / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis; D008722:Methods; D010743:Phospholipids / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013237:Stereoisomerism
D005227:Fatty Acids; D010743:Phospholipids