title: Suppression of experimental crescentic glomerulonephritis by interleukin-10 gene transfer.
abstract: BACKGROUND
  Investigated were effects of overexpression of interleukin-10 (IL-10) on the outcome and progression of crescentic glomerulonephritis in Wistar-Kyoto (WKY) rats.
  
  
  METHODS
  Rats were singly or simultaneously injected with antiglomerular basement membrane (a-GBM) antibody and adenoviral vector encoding rat IL-10 (Ad-rIL-10) or LacZ (Ad-LacZ) (3 x 1010 pfu/rat) intravenously, and were sacrificed at day 7. Their kidneys and other organs were isolated and examined by histology and immunohistochemistry. The In vivo expression of IL-10 mRNA in the liver of Ad-rIL-10-injected rats was confirmed by both reverse transcription-polymerase chain reaction (RT-PCR) and ribonuclease protection assay analysis and its translated protein was measured in the serum by enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  The exogenous IL-10 mRNA was strongly expressed in the liver in a dose-dependent manner and was intense at days 4 and 7 but was less intense at day 14. Ad-rIL-10 treatment significantly reduced the incidence of glomerular crescent formation from 67%+/- 1.9% in a-GBM antibody-treated group or 69.8%+/- 1.9% in a-GBM antibody + Ad-LacZ-treated group to 21.6%+/- 1.8% (P < 0.001), the glomerular infiltration of macrophages from 35.7 +/- 6.3 cell s/gcs (a-GBM antibody) or 37.6 +/- 8.6 cells/gcs (both a-GBM antibody + Ad-LacZ) to 17.9 +/- 5.5 cells/gcs (P < 0.001), that of major histocompatibility complex (MHC) class II-positive cells from 14.4 +/- 5.3 cells/gcs (a-GBM antibody) or 15 +/- 4.6 cells/gcs (a-GBM antibody + Ad-LacZ) to 5.7 +/- 2.3 cells/gcs (P < 0.0001) at day 7, the glomerular and immune tissue expression of IL-1beta mRNA, as well as the proteinuria from 159.0 +/- 22.7 mg/24 hours (a-GBM antibody) or 166 +/- 28 mg/24 hours (a-GBM antibody + Ad-LacZ) to 42.2 +/- 35.2 mg/24 hours (P < 0.01) at day 7. The serum creatinine and blood urea nitrogen levels were also reduced from 2.8 +/- 0.1 mg/dL (a-GBM antibody) or 2.8 +/- 0.1 mg/dL (a-GBM antibody + Ad-LacZ) to 1.0 +/- 0.1 mg/dL (P < 0.001) and from 63.2 +/- 8.9 mg/dL (a-GBM antibody) or 61.3 +/- 5.2 mg/dL (a-GBM antibody + Ad-LacZ) to 27.0 +/- 4.5 mg/dL (P < 0.001), respectively. However, the glomerular accumulation of CD8+ T cells was unaffected: 5.4 +/- 1.1 cells/gcs (a-GBM antibody + Ad-rIL-10), 5.9 +/- 1.5 cells/gcs (a-GBM antibody), and 5.8 +/- 1.1 cells/gcs (a-GBM antibody + Ad-LacZ) (P= NS).
  
  
  CONCLUSIONS
  IL-10 gene transfer significantly attenuated the glomerular lesions and injury in the anti-GBM crescentic glomerulonephritis of WKY rats.
authors.affiliation: Department of Structural Pathology, Institute of Nephrology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan.
authors.forename: Adel G A
authors.initials: AG
authors.lastname: El-Shemi
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Fujinaka
authors.forename: Asako
authors.initials: A
authors.lastname: Matsuki
authors.forename: Junichi
authors.initials: J
authors.lastname: Kamiie
authors.forename: Pavel
authors.initials: P
authors.lastname: Kovalenko
authors.forename: Zhenyun
authors.initials: Z
authors.lastname: Qu
authors.forename: Vladimir
authors.initials: V
authors.lastname: Bilim
authors.forename: Goro
authors.initials: G
authors.lastname: Nishimoto
authors.forename: Eishin
authors.initials: E
authors.lastname: Yaoita
authors.forename: Yuatka
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Ignacio
authors.initials: I
authors.lastname: Anegon
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yamamoto
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D019867:Anti-Glomerular Basement Membrane Disease / Q000378:metabolism* / Q000473:pathology*; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D018450:Disease Progression; D005260:Female; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics*; D007678:Kidney Glomerulus / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism; D011507:Proteinuria / Q000652:urine; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D011921:Rats, Inbred WKY; D013154:Spleen / Q000378:metabolism
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger; D016753:Interleukin-10; D003404:Creatinine