title: Permanent repigmentation of piebaldism by erbium:YAG laser and autologous cultured epidermis.
abstract: BACKGROUND
  Several surgical techniques have been proposed for the treatment of piebaldism. These procedures, however, are poorly suited for the treatment of large leucodermal lesions, can cause scars and require multiple donor sites. Recently, it has been reported that autologous cultured epidermis induces scarless repigmentation of large vitiligo lesions, using a single small donor site.
  
  
  OBJECTIVE
  To induce permanent repigmentation of large achromic lesions in patients suffering from piebaldism by means of autologous cultured epidermal grafts using a rapid, simple and non-invasive surgical procedure.
  
  
  METHODS
  Six patients with piebaldism were enrolled in this study. Achromic epidermis was removed by means of appropriately set erbium:YAG laser and autologous cultured epidermal grafts were applied on to the recipient bed. Melanocyte content was evaluated by 3,4-dihydroxyphenylalanine reaction. The percentage of repigmentation was calculated using a semiautomatic image analysis system.
  
  
  RESULTS
  Autologous cultured epidermis, bearing a controlled number of melanocytes, induced repigmentation of all piebald lesions. The mean percentage repigmentation was 95.45% (2791.5 cm2 repigmented/2924.2 cm2 transplanted).
  
  
  CONCLUSIONS
  Autologous cultured epidermal grafts induce permanent and complete repigmentation of piebald lesions, in the absence of scars. Erbium:YAG laser surgery is a rapid and precise tool for disepithelialization, hence allowing treatment of large piebald lesions during a single surgical operation.
authors.affiliation: Laboratory of Tissue Engineering, Istituto Dermopatico dell'Immacolata, Rome, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Guerra
authors.forename: G
authors.initials: G
authors.lastname: Primavera
authors.forename: D
authors.initials: D
authors.lastname: Raskovic
authors.forename: G
authors.initials: G
authors.lastname: Pellegrini
authors.forename: O
authors.initials: O
authors.lastname: Golisano
authors.forename: S
authors.initials: S
authors.lastname: Bondanza
authors.forename: S
authors.initials: S
authors.lastname: Kuhn
authors.forename: P
authors.initials: P
authors.lastname: Piazza
authors.forename: A
authors.initials: A
authors.lastname: Luci
authors.forename: F
authors.initials: F
authors.lastname: Atzori
authors.forename: M
authors.initials: M
authors.lastname: De Luca
mesh_terms: D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D002648:Child; D003131:Combined Modality Therapy / Q000379:methods; D004817:Epidermis / Q000503:physiopathology; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000637:transplantation*; D053685:Laser Therapy / Q000379:methods*; D008297:Male; D016116:Piebaldism / Q000503:physiopathology / Q000601:surgery / Q000628:therapy*; D012880:Skin Pigmentation / Q000502:physiology*; D014182:Transplantation, Autologous