Posttranscriptional gene silencing in plants.
Double-stranded RNA when introduced into cells results in severe reduction of the target mRNA. This phenomenon is known as posttranscriptional gene silencing in plants and RNA interference in animals. Hairpin RNA-mediated gene silencing exploits this cellular mechanism. A convenient way of generating hairpin constructs is to use generic vectors such as pHANNIBAL and pHELLSGATE, vectors based on the Gateway technology. These vectors are suitable for high-throughput gene silencing, and the silencing effect is stably inherited over many generations.
Wesley
Susan Varsha
SV
CSIRO Plant Industry, Canberra, Australia.
Helliwell
Chris
C
Wang
Ming-Bo
MB
Waterhouse
Peter
P
D003001:Cloning, Molecular / Q000379:methods; D018744:DNA, Plant / Q000235:genetics*; D020868:Gene Silencing / Q000502:physiology*; D005822:Genetic Vectors; D010944:Plants / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012330:RNA, Double-Stranded / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D015183:Restriction Mapping / Q000379:methods
D018744:DNA, Plant; D012330:RNA, Double-Stranded; D018749:RNA, Plant