[Comparative assessment of the human cell viability ex vivo in Hanks solutions in distilled and Penta water].
Assessment of human colon and lung mucosa cell viability was performed in Hanks salt media prepared separately with distilled and patented Penta water. The cell viability in the suspension was estimated by fluorescence intensity of propidium iodide, a DNA specific dye, that is an indicator of DNA structure intactness or damage. The experiments were conducted with the flow cytometry technique. The histogram analysis showed that 2-hour incubation of the cells in Hanks salt medium prepared with distilled water resulted in an increase of the number of the apoptotic cells with a respective decrease of the number of the intact cells (approximately 2- and 4-fold in the suspensions of the colon and lung mucosa cells respectively). A similar experiment with Hanks salt medium prepared with Penta water resulted in a less marked increase of the viability of the apoptotic cells that did not exceed 20 and 50% for the colon and lung mucosa cells respectively. The findings showed that viability of the cells ex vivo was significantly higher when Penta water was used as a solvent for preparing Hanks salt media as compared to distilled water. The result is important for ex vivo experiments since maximum preservation of the DNA structure minimizes the number of possible experimental inaccurate and consequently erroneous conclusions. Furthermore, the fact of pathologic process inhibition in cells isolated from various human tissues in Penta-based salt media is in favour of using Penta water as a solvent for nutritional ingredients in ex vivo maintenance of human tissues for transplantation as compared to distilled water.
Bogush
T A
TA
N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow.
Laktionov
K K
KK
Bogush
E A
EA
Polotskiĭ
B E
BE
D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D007420:Intestine, Large / Q000166:cytology*; D007552:Isotonic Solutions / Q000737:chemistry*; D008168:Lung / Q000166:cytology*; D014867:Water / Q000737:chemistry*
D003470:Culture Media; C072670:Hanks Balanced Salt Solution; D007552:Isotonic Solutions; D014867:Water