The preventive inhibition of chondroitin sulfate against the CCl4-induced oxidative stress of subcellular level.
Our work in this study was made in the microsomal fraction to evaluate the lipid peroxidation by measuring superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and malondialdehyde (MDA) and to elucidate the preventive role of CS in the CCl4-induced oxidative stress. The excessive lipid peroxidation by free radicals derived from CCl4 leads to the condition of oxidative stress which results in the accumulation of MDA. MDA is one of the end-products in the lipid peroxidation process and oxidative stress. MDA, lipid peroxide, produced in this oxidative stress causes various diseases related to aging and hepatotoxicity, etc. Normal cells have a number of enzymatic and nonenzymatic endogenous defense systems to protect themselves from reactive species. The enzymes in the defense systems, for example, are SOD, CAT, and GPx. They quickly eliminate reactive oxygen species (ROS) such as superoxide anion free radical *O2(-), hydrogen peroxide H2O2 and hydroxyl free radical *OH. CS inhibited the accumulation of MDA and the deactivation of SOD, CAT and GPx in the dose-dependent and preventive manner. Our study suggests that CS might be a potential scavenger of free radicals in the oxidative stress originated from the lipid peroxidation of the liver cells of CCl4-treated rats.
Lee
Jin Young
JY
Department of Biochemistry, College of Medicine, Inje University, 633-165, Gaegum-Dong, Busanjin-Gu, Busan, 614-735, Korea.
Lee
Sang Hun
SH
Kim
Hee Jin
HJ
Ha
Jong-Myung
JM
Lee
Sang-Hyun
SH
Lee
Jae-Hwa
JH
Ha
Bae Jin
BJ
D000818:Animals; D002251:Carbon Tetrachloride / Q000037:antagonists & inhibitors; D002809:Chondroitin Sulfates / Q000494:pharmacology*; D005260:Female; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
D002809:Chondroitin Sulfates; D002251:Carbon Tetrachloride