Identification of Botrytis cinerea susceptibility loci in Arabidopsis thaliana.
Botrytis cinerea is a major pathogen of fruit and vegetable crops causing both pre- and post-harvest grey mould. We have analysed 16 Arabidopsis thaliana ecotypes for natural variation in B. cinerea susceptibility. Susceptibility was associated with lower camalexin accumulation, and three ecotypes (Cape Verdi Islands (Cvi-0), Slavice (Sav-0) and Kindalville (Kin-0)) showed differential susceptibility to the two B. cinerea isolates used. Subsequently, to better understand the genetic control of grey mould disease, we assayed the Arabidopsis Landsberg erecta (Ler) x Columbia (Col-0) recombinant inbred population with the two isolates, and identified multiple small-to-medium-effect quantitative trait loci (QTL) governing susceptibility. Interestingly, the QTL for each isolate are distinct, suggesting that different mechanisms govern defence against these two isolates. Two QTL for each isolate exhibited epistatic interactions with specific allele combinations generating heightened B. cinerea susceptibility.
Denby
Katherine J
KJ
Department of Molecular and Cell Biology, University of Cape Town, Private Bag Rondebosch 7700, South Africa.
Kumar
Pavan
P
Kliebenstein
Daniel J
DJ
D017360:Arabidopsis / Q000145:classification / Q000235:genetics* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D020171:Botrytis / Q000254:growth & development*; D007113:Immunity, Innate / Q000187:drug effects / Q000235:genetics; D007211:Indoles / Q000378:metabolism; D009579:Nitrobenzoates / Q000494:pharmacology; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D040641:Quantitative Trait Loci; D013045:Species Specificity; D013844:Thiazoles / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000094162:Phytoalexins
D029681:Arabidopsis Proteins; D007211:Indoles; D009579:Nitrobenzoates; D013844:Thiazoles; D014157:Transcription Factors; C482738:BOTRYTIS SUSCEPTIBLE1 protein, Arabidopsis; C102405:camalexin; D000094162:Phytoalexins; C018425:acifluorfen