Regulation of stearoyl-CoA desaturase-1 after central and peripheral nerve lesions.
BACKGROUND
Interruption of mature axons activates a cascade of events in neuronal cell bodies which leads to various outcomes from functional regeneration in the PNS to the failure of any significant regeneration in the CNS. One factor which seems to play an important role in the molecular programs after axotomy is the stearoyl Coenzyme A-desaturase-1 (SCD-1). This enzyme is needed for the conversion of stearate into oleate. Beside its role in membrane synthesis, oleate could act as a neurotrophic factor, involved in signal transduction pathways via activation of protein kinases C.


RESULTS
In situ hybridization and immunohistochemistry demonstrated a strong up-regulation of SCD at mRNA and protein level in regenerating neurons of the rat facial nucleus whereas non-regenerating Clarke's and Red nucleus neurons did not show an induction of this gene.


CONCLUSIONS
This differential expression points to a functionally significant role for the SCD-1 in the process of regeneration.
Breuer
Sebastian
S
Department of Neurology, University Medical School, Pauwelsstr 30, D-52074 Aachen, Germany. gotzes@rwth-aachen.de
Pech
Katrin
K
Buss
Armin
A
Spitzer
Christoph
C
Ozols
Juris
J
Hol
Elly M
EM
Heussen
Nicole
N
Noth
Johannes
J
Schwaiger
Franz-Werner
FW
Schmitt
Andreas B
AB
D000818:Animals; D019771:Axotomy; D002490:Central Nervous System / Q000201:enzymology* / Q000293:injuries / Q000473:pathology; D018450:Disease Progression; D020220:Facial Nerve Injuries / Q000201:enzymology / Q000473:pathology; D007002:Hypoglossal Nerve / Q000201:enzymology / Q000473:pathology; D061228:Hypoglossal Nerve Injuries; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000378:metabolism; D009416:Nerve Regeneration / Q000502:physiology*; D009474:Neurons / Q000201:enzymology / Q000473:pathology; D017933:Peripheral Nervous System / Q000201:enzymology* / Q000293:injuries / Q000473:pathology; D011149:Pons / Q000201:enzymology / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012012:Red Nucleus / Q000201:enzymology / Q000473:pathology; D013119:Spinal Cord Injuries / Q000201:enzymology / Q000473:pathology; D013230:Stearoyl-CoA Desaturase / Q000378:metabolism*; D020196:Trauma, Nervous System / Q000201:enzymology* / Q000473:pathology; D015854:Up-Regulation
D007527:Isoenzymes; D012333:RNA, Messenger; D013230:Stearoyl-CoA Desaturase