Light traffic: photo-crosslinking a novel transport system.
The Tat protein transporter is found in the membranes of many bacteria and in plant chloroplasts. This highly unusual transport machine moves folded and often oligomeric substrate proteins across energy-conserving membranes. A recent paper reports the first use of a photo-crosslinking approach to dissect the early recognition events between the transporter and its substrate.
Palmer
Tracy
T
Department of Molecular Microbiology, John Innes Centre, Norwich, UK. tracy.palmer@bbsrc.ac.uk
Sargent
Frank
F
Berks
Ben C
BC
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001120:Arginine / Q000378:metabolism; D001419:Bacteria / Q000166:cytology; D001426:Bacterial Proteins / Q000378:metabolism; D001692:Biological Transport; D002736:Chloroplasts / Q000378:metabolism; D016384:Consensus Sequence; D003432:Cross-Linking Reagents / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism; D015696:Gene Products, tat / Q000378:metabolism*; D008027:Light; D008239:Lysine / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D010940:Plant Proteins / Q000378:metabolism; D017510:Protein Folding; D021382:Protein Sorting Signals; D021381:Protein Transport / Q000235:genetics; D013379:Substrate Specificity; D020524:Thylakoids / Q000378:metabolism
D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D029968:Escherichia coli Proteins; D015696:Gene Products, tat; D026901:Membrane Transport Proteins; D010940:Plant Proteins; D021382:Protein Sorting Signals; D001120:Arginine; D008239:Lysine