title: Two cDNAs from the purple sea urchin, Strongylocentrotus purpuratus, encoding mosaic proteins with domains found in factor H, factor I, and complement components C6 and C7.
abstract: The vertebrate complement system is composed of about 30 serum and cell surface proteins that make up three activation pathways, a lytic pathway, and a set of proteins that regulate complement. Regulatory proteins are required for host protection against autologous complement attack and to control the amplification feedback loop of the alternative pathway. Purple sea urchin, Strongylocentrotus purpuratus, homologues of complement C3 (SpC3) and factor B (SpBf) have been identified, suggesting the presence of an alternative complement pathway. This implies that echinoderms require a complement regulatory system for the same reasons that it is required in higher vertebrates. Two cDNAs, Sp5 and Sp5013, have been characterized from coelomocytes and the deduced structures of the encoded mosaic proteins, SpCRL ( S. p urpuratus complement related protein, long form) and SpCRS ( short form), have domains that are also found in regulatory proteins such as factor H and factor I and the terminal pathway components C6 and C7. These domains include multiple short consensus repeats, a fucolectin domain, Ser/Thr/Pro-rich regions, a Cys-rich region, and a factor I-membrane attack complex domain. The genes are constitutively expressed in all tissues of the sea urchin and are not induced in response to immune challenge. Multiple bands of varying intensity on both genome blots and RNA blots suggest that Sp5 and Sp5013 are members of a small gene family and that they might undergo alternative splicing. Based on the domains present in SpCRL and SpCRS, they might be either examples of complement regulatory proteins or members of the terminal pathway of complement.
authors.affiliation: Graduate Program in Genetics, The Institute of Biomedical Sciences, George Washington University, Washington, DC 20052, USA.
authors.forename: Keri A
authors.initials: KA
authors.lastname: Multerer
authors.forename: L Courtney
authors.initials: LC
authors.lastname: Smith
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003183:Complement C6 / Q000235:genetics*; D003184:Complement C7 / Q000235:genetics*; D017242:Complement Factor H / Q000235:genetics*; D003165:Complement System Proteins / Q000276:immunology*; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000235:genetics*; D005340:Fibrinogen / Q000235:genetics*; D007107:Immune System / Q000502:physiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D012617:Sea Urchins / Q000235:genetics* / Q000276:immunology; D017386:Sequence Homology, Amino Acid
chemical_list: D003183:Complement C6; D003184:Complement C7; D018076:DNA, Complementary; D012333:RNA, Messenger; D017242:Complement Factor H; D005340:Fibrinogen; D003165:Complement System Proteins