Transcellular transport of 4-iodo-L-meta-tyrosine via system L across monolayers of kidney epithelial cell line LLC-PK1.
The substance 4-[(125)I]iodo-L-meta-tyrosin (4-[(125)I]mTyr) is a radioiodinated amino acid that exhibits high in vivo stability and rapid renal elimination in vivo. We investigated transport of 4-[(125)I]mTyr in LLC-PK(1) (porcine kidney epithelial cell line) monolayers grown on collagen-coated, micro-porous membrane filters. We found that 4-[(125)I]mTyr transport in LLC-PK(1) cells was carrier-mediated and sodium-independent, and that 4-[(125)I]mTyr transport was similar to that of L-Tyr and 3-iodo-alpha-methyl-L-tyrosine. The results of the inhibition experiments suggest that 4-[(125)I]mTyr transport is predominantly mediated by a L-type amino acid transporter 1-like porcine homologue (a component of system L) in both basolateral and apical membrane.
Shikano
Naoto
N
Department of Radiological Sciences, Ibaraki Prefectural University of Health Sciences, 4669-2 Ami Ami-machi, Inashiki-gun, Ibaraki 300-0394, Japan. sikano@ipu.ac.jp
Kawai
Keiichi
K
Nakajima
Syuichi
S
Kubodera
Akiko
A
Kubota
Nobuo
N
Ishikawa
Nobuyoshi
N
Saji
Hideo
H
D027062:Amino Acid Transport System L / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics*; D007668:Kidney / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D013552:Swine; D014443:Tyrosine / Q000493:pharmacokinetics*
D027062:Amino Acid Transport System L; D007457:Iodine Radioisotopes; D019275:Radiopharmaceuticals; D014443:Tyrosine