7-Nitroindazole upregulates phosphorylated cAMP response element binding protein, polysialylated-neural cell adhesion molecule and tryptophan hydroxylase expression in the adult rat hippocampus.
We examined the effect of nitric oxide (NO) on the expression of phosphorylated cAMP response element binding (pCREB) protein and on the polysialylated form of the neural cell adhesion molecule (PSA-NCAM), in the hippocampus of the adult rat, by administering 7-nitroindazole (7-NI) for 5 days, to block neuronal nitric oxide synthase (nNOS) activity. It was found that PSA-NCAM and pCREB immunoreactive cells were increased two-fold in the dentate gyrus by this treatment. In addition, the immunoreactivities of serotonin (5-HT, 5-hydroxytryptamine), known to be involved in neural plasticity, and of tryptophan hydroxylase immunoreactive-fibers in the dentate gyrus were both upregulated by 7-NI. Our results indicate that nNOS inhibition upregulates neural plasticity and increases 5-HT in the rat hippocampus, and suggest the existence of regulatory relationships between NO, neural plasticity and 5-HT.
Park
Chan
C
Department of Anatomy, Medical Science and Engineering Research Center for Reactive Oxygen Species, College of Medicine, Kyunghee University, Hoeki-Dong 1, Dongdaemun-Gu, Seoul 130-701, South Korea.
Cho
Keumok
K
Ryu
Jong Hoon
JH
Shin
Ki Soon
KS
Kim
Junghye
J
Ahn
Heekyung
H
Huh
Youngbuhm
Y
D000818:Animals; D002452:Cell Count / Q000379:methods; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D007191:Indazoles / Q000494:pharmacology*; D008297:Male; D039842:Neural Cell Adhesion Molecule L1 / Q000378:metabolism*; D011903:Raphe Nuclei / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012794:Sialic Acids / Q000378:metabolism*; D014365:Tryptophan Hydroxylase / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
D017362:Cyclic AMP Response Element-Binding Protein; D004791:Enzyme Inhibitors; D007191:Indazoles; D039842:Neural Cell Adhesion Molecule L1; D012794:Sialic Acids; C103469:polysialyl neural cell adhesion molecule; D014365:Tryptophan Hydroxylase; C080122:7-nitroindazole