In vivo antitumor and immunomodulation activities of different molecular weight lambda-carrageenans from Chondrus ocellatus.
lambda-Carrageenan is a sulfated galactan isolated from some red algae and have been reported to have many kinds of biological activities. lambda-Carrageenan from Chondrus ocellatus, an important economic alga in China and many other parts of the world, was degraded by microwave, and obtained five products that have different molecular weight: 650, 240, 140, 15, 9.3 kDa. Analytical results confirmed that microwave degradation might not change the chemical components and structure of polysaccharides under certain condition. In this study, tumor-inhibiting activities, weight of immune organ, nature killer cells activity, lymphocyte proliferation ratio and pathological slice of spleen and tumor cells from the control group and lambda-carrageenan-treated mice of transplanted S180 and H22 tumor were investigated. The results indicated that the five lambda-carrageenan samples all showed antitumor and immunomodulation activities in different degree. Molecular weight of polysaccharides had notable effect on the activities. In addition, their antitumor and immunomodulation have some relevance and the five lambda-carrageenans probably inhibited tumor by means of activating the immunocompetence of the body. Among all the experiment results, samples with the highest activities are PC4 and PC5 whose molecular weight are 15 and 9.3 kDa.
Zhou
Gefei
G
Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
Sun
YuePing
Y
Xin
Hua
H
Zhang
Yuna
Y
Li
Zhien
Z
Xu
Zuhong
Z
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002351:Carrageenan / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D044723:Chondrus / Q000737:chemistry*; D007155:Immunologic Factors / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects; D051379:Mice; D008813:Mice, Inbred ICR; D008872:Microwaves; D008970:Molecular Weight; D009368:Neoplasm Transplantation; D009929:Organ Size / Q000187:drug effects; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D007155:Immunologic Factors; D002351:Carrageenan