Improved production of bioactive glucosylmannosyl-glycerolipid by sponge-associated Microbacterium species.
The marine Microbacterium species HP2 (DSM 12583), isolated from the sponge Halichondria panicea, is able to produce a glucosylmannosyl-glycerolipid when grown on a complex medium with glucose. Optimizing the carbon sources in shake flask experiments has shown that glycerol affords the highest specific glycoglycerolipid production. The product yield approached 300 mg/L or 25 mg/g biomass upon scaling up in a 40-L bioreactor volume. The native diglycosyl-glycerolipid GGL.2 strongly inhibited growth of the tumor cell lines HM02 and Hep G2 (50% inhibition at 0.4 to 3 microg/mL), while the related deacylated compound (GG.2) showed a potent anti-tumor-promoting activity.
Lang
Siegmund
S
Technical University of Braunschweig, Institute of Biochemistry and Biotechnology, Biotechnology Group, Spielmannstr. 7, D-38106 Braunschweig, Germany. s.lang@tu-bs.de
Beil
Winfried
W
Tokuda
Harukumi
H
Wicke
Corinna
C
Lurtz
Verena
V
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D019149:Bioreactors; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006017:Glycolipids / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology*; D008835:Micrococcaceae / Q000737:chemistry*; D011161:Porifera / Q000382:microbiology*
D000970:Antineoplastic Agents; D006017:Glycolipids