title: Simultaneous determination of the major isosteroidal alkaloids and their glucosides in the bulbs of Fritillaria by high performance liquid chromatography coupled with evaporative light scattering detection.
abstract: OBJECTIVE
  To establish an HPLC-ELSD method for the simultaneous determination of five major bioactive isosteroidal alkaloids and gluco-alkaloids in the bulbs of Fritillaria namely peimissine, imperialine, sinpeinine A, imperialine-3 beta-glucoside and yibeinoside A.
  
  
  METHODS
  A Nova-Pak C18 column (150 mm x 3.9 mm ID) was used. The chromatography was carried out with a linear gradient programming. The mobile phase was acetonitrile-water (containing 0.1% diethylamine) and the flow rate was 1.0 mL.min-1.
  
  
  RESULTS
  The linear range of peimissine was 13.1-288.2 mg.L-1 (r2 = 0.9975), imperialine-3 beta-glucoside 7.7-169.4 mg.L-1 (r2 = 0.9993), yibeinoside A 7.3-160.6 mg.L-1 (r2 = 0.9997), imperialine 16.5-363.0 mg.L-1 (r2 = 0.9992), sinpeinine A 8.7-191.4 mg.L-1 (r2 = 0.9942).
  
  
  CONCLUSIONS
  The method is accurate with overall intra- and inter-day variation less than 5% and recovery more than 95%. The method was successfully applied to analyze five major bioactive alkaloids and gluco-alkaloids in three Fritillaria bulbs.
authors.affiliation: Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210009, China. lipingli@public1.ptt.js.cn
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Ling-jie
authors.initials: LJ
authors.lastname: Zeng
authors.forename: Song-lin
authors.initials: SL
authors.lastname: Li
authors.forename: Zhi-ming
authors.initials: ZM
authors.lastname: Bi
authors.forename: Ge
authors.initials: G
authors.lastname: Lin
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D002595:Cevanes / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D031410:Fritillaria / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D002595:Cevanes; D004365:Drugs, Chinese Herbal; D005960:Glucosides; C052510:peimisine; C069537:sinpeinine A; C069538:yibeinoside A; C047331:peiminine