title: The importance of cleaning for the overall results of processing endoscopes.
abstract: Reprocessing comprises three steps: cleaning, disinfection and-if required-sterilisation. While the extents of disinfection and of sterilisation are quantitatively defined, there are only imprecise (qualitative) definitions of cleaning. There are two main reasons for accurate cleaning. First organic and inorganic materials that remain on inner and outer surfaces will interfere with the efficacy of the disinfectants. In case of endoscopes this will lead to channel blockages; they remain undisinfected. Second the bioburden found on endoscopes after use can be very high. Data available demonstrate that a bacterial burden of up to 10(9)cfu/endoscope channel can be expected. Therefore it is necessary to perform a thorough cleaning. Studies using endoscopes showed a reduction in microbial counts by a factor of approximately 10(4) by cleaning (manual and mechanical). Therefore in 2001 the German Society of Hospital Hygiene (DGKH) specified its requirements and recommendations for determining cleaning efficacy separately from those for disinfection. Cleaning and disinfecting can be done manually or mechanically, but it seems impossible to validate manual processes. However our studies in two different washer-disinfectors (WD) showed differences in cleaning efficacy. The tested cleaning processes showed different efficacies. Not all cleaning processes showed better results than water alone with regard to visible cleanliness and to a microbiological reduction E. faecium. Our results show that the evaluation of cleanliness exclusively by visible inspection is not sufficient, particular for the lumens of endoscopes. The results also show that a cleaning process may be very effective also in reducing micro-organisms present.
authors.affiliation: Charité-Universitätsmedizin Berlin, Technische Hygiene, Campus Benjamin Franklin, Hindenburgdamm 27, 12203 Berlin, Germany. heike.martiny@medizin.fu-berlin.de
authors.forename: H
authors.initials: H
authors.lastname: Martiny
authors.forename: H
authors.initials: H
authors.lastname: Floss
authors.forename: B
authors.initials: B
authors.lastname: Zühlsdorf
mesh_terms: D015169:Colony Count, Microbial; D004203:Disinfection / Q000379:methods*; D019723:Endoscopes / Q000382:microbiology*; D017743:Equipment Reuse; D005858:Germany; D006801:Humans; D011786:Quality Control