[Determination of calycosin-7-O-beta-D-glucopyranoside in radix astragali by HPLC].
OBJECTIVE
TO establish a RP-HPLC method for the determination of calycosin-7-O-beta-D-glucopyranoside in Radix Astragali, and to analyse the calycosin-7-O-beta-D-glucopyranoside content of ten samples of Radix Astragali, collected from different regions.


METHODS
A Polaris C18(250 mm x 4.6 mm, 5 microns) column was used and a mixture of methanol-water (30:70) was used as the mobile phase at a flow rate of 1.0 mL.min-1. The column temperature was 25 degrees C and the UV detection wavelength was 254 nm.


RESULTS
The calibration curve was in good linearity over the range of 0.0106-2.12 micrograms with the regression equation Y = 3035. 97 X - 14.85(r = 0.9999). The average recovery was 95.8% (n = 5, RSD = 1.3%).


CONCLUSIONS
The method is simple, quick, sensitive and reproducible. In all of the samples, the calycosin-7-O-beta-D-glucopyranoside contents differ markedly.
Luo
Chun-xia
CX
Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, China.
Lin
Ping-chuan
PC
Gu
Li-hua
LH
Wu
Tao
T
Wu
Da-zheng
DZ
Wang
Zheng-tao
ZT
Hu
Zhi-bi
ZB
D027885:Astragalus propinquus / Q000737:chemistry* / Q000145:classification; D002681:China; D002851:Chromatography, High Pressure Liquid; D017753:Ecosystem; D005960:Glucosides / Q000032:analysis*; D007529:Isoflavones / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D013045:Species Specificity
D005960:Glucosides; D007529:Isoflavones; C488311:calycosin-7-O-glucopyranoside