title: [The dynamic expression of TNF-alpha mRNA of hypertrophic scars and its roles].
abstract: OBJECTIVE
  To investigate the role of tumor necrosis factor alpha: TNF-alpha in the hypertrophic scar and find a valid way to treat the hypertrophic scars with gene therapy.
  
  
  METHODS
  TNF-alpha mRNA expression was tested with a semiquantitative reverse polymerase chain reation (RT-PCR) method in the different period of a hypertrophic scar.
  
  
  RESULTS
  There was a significant increase of TNF-alpha mRNA as the hypertrophic scar was getting mature (P < 0.01). However, the ratios of TNF-alpha mRNA was significantly in a lower level than the normal scar.
  
  
  CONCLUSIONS
  The results suggest that TNF-alpha may play an important role in normal would healing, but the hypertrophic scar may result from the shortage of the TNF-alpha. This indicates that to increase the TNF-alpha with gene therapy may be a good way to treat the hyphertrophic scar.
authors.affiliation: Department of Plastic Surgery, Affiliated Hospital of Second Military Medical University of PLA, Shanghai 200003, China.
authors.forename: Jian-lin
authors.initials: JL
authors.lastname: Zhang
authors.forename: Zi-hao
authors.initials: ZH
authors.lastname: Lin
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.forename: Xiang-bin
authors.initials: XB
authors.lastname: Yuan
authors.forename: Yao-zhong
authors.initials: YZ
authors.lastname: Zhao
authors.forename: Jian-ming
authors.initials: JM
authors.lastname: Wu
authors.forename: Xiao-hai
authors.initials: XH
authors.lastname: Zhu
authors.forename: Hong
authors.initials: H
authors.lastname: Wu
mesh_terms: D000328:Adult; D017439:Cicatrix, Hypertrophic / Q000235:genetics* / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha