Colonic ulceration and increase of neutrophil elastase activity in the acetic acid-induced colitis model in Syrian hamsters.
A novel colitis model using Syrian hamsters was developed. Colitis was induced by intracolonic administration of 1% acetic acid, and the ulcer area, tissue myeloperoxidase (MPO) activity, and luminal neutrophil elastase (NE) activity of the colon were determined at 1, 3, 8, 24 and 48 hr after colitis induction. The histopathological changes of the colon were also examined in this model. An increase of tissue MPO activity and NE activity was evident at 3 hr after induction of colitis, peaked at 24 hr, and decreased subsequently. The increase of luminal NE activity was well correlated with the colonic ulcer area. In histopathological examination, ulceration, erosion, crypt abscesses, neutrophil infiltration, hemorrhage, and edema were seen. The effects of prednisolone were examined to evaluate the adequacy of our colitis model. Syrian hamsters were treated orally with prednisolone at 18 and 1 hr before and at 6 hr after induction of colitis, and the ulcer area, tissue MPO activity, and luminal NE activity were evaluated at 24 hr after colitis induction. Prednisolone therapy had little effect on the tissue MPO activity. However, the NE activity of the prednisolone-treated group was significantly decreased. In addition, although prednisolone did not significantly decrease the ulcer area, a tendency toward decrease was noted. We conclude that this new model of experimental colitis in Syrian hamsters is useful for investigating the pathophysiology of colitis, especially useful for studying the relationship between colitis and NE activity.
Hirota
Yasushi
Y
Fukui Safety Research Laboratories, Ono Pharmaceutical Co., Ltd., Japan.
Suzuki
Mikie
M
Yamaguchi
Kojiro
K
Fujita
Tsuneo
T
Katsube
Nobuo
N
D019342:Acetic Acid; D000818:Animals; D003093:Colitis, Ulcerative / Q000139:chemically induced* / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D003106:Colon / Q000473:pathology*; D006224:Cricetinae; D004195:Disease Models, Animal; D006652:Histological Techniques; D019272:Leukocyte Elastase / Q000378:metabolism*; D008647:Mesocricetus; D009195:Peroxidase / Q000378:metabolism*; D011239:Prednisolone / Q000627:therapeutic use*; D013997:Time Factors
D011239:Prednisolone; D009195:Peroxidase; D019272:Leukocyte Elastase; D019342:Acetic Acid