title: [Association between a genomic polymorphism within the CD14 locus and severe sepsis susceptibility as well as prognosis in patients after extensive burns].
abstract: OBJECTIVE
  To investigate the relation of a lipopolysaccharide receptor CD14C-159T gene polymorphism to severe sepsis susceptibility and prognosis in patients with extensive burns.
  
  
  METHODS
  The study group consisted of 118 normal controls and 16 patients with burns covering more than 60 percent total body surface area. Typing of each patient for the CD14C-159T gene polymorphism was performed by analyzing restriction fragments of a Hae III-digested DNA fragment obtained using polymerase chain reaction (PCR-RFLP). Genotypes were then related to the susceptibility and mortality rate of severe sepsis.
  
  
  RESULTS
  The overall allele frequency (C 43.7 percent, T 56.3 percent) and genotype distribution (C homozygous 12.5 percent, C/T 62.5 percent, T homozygous 25.0 percent) were in agreement with the distribution in healthy volunteers. Genotype distribution in patients with severe sepsis was different from that in patients with an uncomplicated clinical course. Development of severe sepsis was increased in patients homozygous for the allele T (71.4 percent) than that of the non- sepsis patients (44.4 percent).
  
  
  CONCLUSIONS
  The single base pair polymorphism at position-159 in the CD14 gene promoter might influence the development of severe sepsis in patients with extensive burns.
authors.affiliation: Burns Institute, 304th Hospital of PLA, Beijing 100037, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lin
authors.forename: Yong-ming
authors.initials: YM
authors.lastname: Yao
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Huang
authors.forename: Xiao-xia
authors.initials: XX
authors.lastname: Hou
authors.forename: Jing-min
authors.initials: JM
authors.lastname: Zhu
authors.forename: Jia-ke
authors.initials: JK
authors.lastname: Chai
mesh_terms: D000328:Adult; D002056:Burns / Q000150:complications*; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011379:Prognosis; D018805:Sepsis / Q000209:etiology / Q000235:genetics* / Q000401:mortality; D015996:Survival Rate
chemical_list: D005819:Genetic Markers; D018950:Lipopolysaccharide Receptors