Matrix metalloproteinase-1 polymorphism of promoter region in sarcoidosis and tuberculosis patients.
OBJECTIVE
Matrix metalloproteinases (MMPs) are associated with several diseases. MMP-1 is one of the interstitial collagenases and the most highly expressed. Recent studies have found that a single nucleotide polymorphism located in the promoter region of the MMP-1 gene affects transcriptional activity. This polymorphism, 1G/2G, has been reported to associate with several malignant tumors and lung diseases. In this study, we investigated whether this polymorphism is associated with sarcoidosis or tuberculosis.


METHODS
Polymerase chain reaction-restriction fragment length polymorphism was used to determine the MMP-1 genotypes of 103 sarcoidosis patients, 105 tuberculosis patients and 106 healthy control subjects.


RESULTS
We found no differences in genotype distributions and allele frequency between sarcoidosis or tuberculosis patients and healthy control subjects. In sarcoidosis patients with ocular involvement, a significant increase in 1G/1G or 1G/2G genotype was observed compared with patients without (p = 0.009, odds ratio (OR) = 3.22, 95% confidence interval (CI): 1.34-7.71). In sarcoidosis patients with three or more organs involved, 1G/1G type tended to increase compared with patients without (p = 0.035, OR = 5.17, 95% CI: 1.12-23.9). In tuberculosis patients with cavity formation, an increasing trend of 1G/1G type was observed compared with patients without (p = 0.064, OR = 7.69, 95% CI: 0.89-66.3).


CONCLUSIONS
Although MMP-1 polymorphism was not associated with onset risk of sarcoidosis and tuberculosis, the clinical characteristics of both diseases were affected by this polymorphism.
Ninomiya
Shigemitsu
S
Department of Internal Medicine and Molecular Science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Aichi, Japan.
Niimi
Takashi
T
Shimizu
Shigeki
S
Sato
Shigeki
S
Achiwa
Hiroyuki
H
Ito
Hidemi
H
Akita
Kenji
K
Maeda
Hiroyoshi
H
Ueda
Ryuzo
R
D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005128:Eye Diseases / Q000473:pathology; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic / Q000235:genetics; D012307:Risk Factors; D012507:Sarcoidosis / Q000235:genetics*; D014397:Tuberculosis, Pulmonary / Q000235:genetics*
D020781:Matrix Metalloproteinase 1