Production of aralin, a selective cytotoxic lectin against human transformed cells, in callus culture of Aralia elata.
Recently we found that aralin, a novel lectin, from Aralia elata selectively induces apoptosis in human virus-transformed and cancer cells compared with normal cells. To overcome the reduction of aralin biosynthesis according to the development of leaves, we established callus tissues from the young shoots. The N-terminal amino acid sequence and immunochemical analyses revealed that ca-aralin, an aralin synthesized in the callus, was structurally identical with aralin except for the glycosylation of the B chain. The selective cytotoxic index (ratio of IC50 value against human lung normal cells versus virus-transformed cells) of ca-aralin was 2-fold higher than that of aralin. Thus, these results suggest that ca-aralin is more useful than aralin as an anticancer agent.
Tomatsu
Makoto
M
Akita Research Institute of Food and Brewing (ARIF), Arayamachi, Akita, Japan. tomatsu@arif.pref.akita.jp
Mujin
Toshiyuki
T
Shibamoto
Norio
N
Tashiro
Fumio
F
Ikuta
Akira
A
D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D027983:Aralia; D002472:Cell Transformation, Viral / Q000187:drug effects; D046508:Culture Techniques; D006801:Humans; D020128:Inhibitory Concentration 50; D037102:Lectins / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D008168:Lung / Q000166:cytology / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018520:Plant Shoots; D054790:Ribosome Inactivating Proteins, Type 2; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000972:Antineoplastic Agents, Phytogenic; D037102:Lectins; D010936:Plant Extracts; D054790:Ribosome Inactivating Proteins, Type 2; C488031:aralin