Humoral immunoreaction induced by TCR DNA vaccine for beta chain of T cell lymphoma.
We exploited the humoral immunoreaction of mice induced by TCR DNA vaccine of beta chain of T cell lymphoma. The plasmids of pcDNA3.1/TCR V beta 8 was constructed. The BALB/c mice were randomly divided into four groups which were pcDNA3.1, pcDNA3.1/TCR V beta 8, pcDNA3.1/TCR V beta 8 + CpG + liposome and phosphorothioate CpG groups with six mice in each group. Vaccines were injected in bilateral musculus quadriceps femoris of mice in the 0, second, and fourth week, respectively. The antibody formation was tested by indirect immuofluorescence in the 0, second, fourth, sixth and eighth weeks, respectively, before and after immunization. Production of antibody against TCR V beta 8 antigen was observed in the groups of pcDNA3.1/TCR V beta 8 and pcDNA3.1/TCR V beta 8 + CpG + liposome. The antibody titer began to rise in the fourth week and attain the maximal value in the sixth week. The antibody titer in the group of pcDNA3.1/TCR V beta 8 + CpG + liposome was higher than that in the group of pcDNA3.1/TCR V beta 8 in the fourth and eighth weeks (both P<0.01); the antibody titer in the group of pcDNA3.1/TCR V beta 8 + CpG + liposome was markedly higher than that in the group of pcDNA3.1/TCR V beta 8 in the sixth week (P<0.001). The result indicate that TCR V beta 8 antigen can induce formation of special antibody in mice. CpG and liposome can improve the humoral immunoreaction induced by TCR V beta 8 gene vaccine.
Zhang
Mingzhi
M
Tumor Department, The First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China. zmz666@sina.com
Dong
Ziming
Z
Li
Jichang
J
Zhao
Guoqiang
G
Chen
Changying
C
Li
Aimin
A
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation / Q000276:immunology*; D000918:Antibody Specificity; D001483:Base Sequence; D019470:Biolistics; D018899:CpG Islands; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D004720:Endonucleases / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D019169:Jurkat Cells; D016399:Lymphoma, T-Cell / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics / Q000276:immunology; D012313:RNA / Q000032:analysis / Q000096:biosynthesis; D011948:Receptors, Antigen, T-Cell / Q000235:genetics* / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D019444:Vaccines, DNA / Q000276:immunology
D018076:DNA, Complementary; D011948:Receptors, Antigen, T-Cell; D019444:Vaccines, DNA; D012313:RNA; D004720:Endonucleases