Sensitive and simple gas chromatographic-mass spectrometric determination for amphetamine in microdialysate and ultrafiltrate samples.
A gas chromatographic-mass spectrometric (GC-MS) method is described for the measurement of amphetamine (AMP) using negative chemical ionization (NCI) mode. Without prior extraction AMP was derivatized with 2,3,4,5,6-pentafluorobenzoyl chloride (PFBC) and simultaneously extracted into toluene. The toluene extract was injected directly into GC-MS equipped with a HP-1 capillary column. The method is simple and more sensitive than most of the previously published methods. The limit of quantification of amphetamine is 25pg (1.4pg on column) with a very limited sample volume (25microl). The within-day precision was from 1.7 to 5.1% and between-day precision was from 2.2 to 7.3%. The method has been used for the measurement of several thousand microdialysate and ultrafiltrate samples and proven reliable.
Xie
Shan
S
Analytical Psychopharmacology Laboratory, Nathan Kline Institute, 140 Old Orangeburg Road, Orangeburg, NY 10962, USA. xies@nki.rfmh.org
Aspromonte
John
J
Balla
Andrea
A
Sershen
Henry
H
Javitt
Daniel C
DC
Cooper
Thomas B
TB
D000661:Amphetamine / Q000032:analysis*; D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008297:Male; D017551:Microdialysis; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000661:Amphetamine