The G protein-coupled receptor rhodopsin in the native membrane.
The higher-order structure of G protein-coupled receptors (GPCRs) in membranes may involve dimerization and formation of even larger oligomeric complexes. Here, we have investigated the organization of the prototypical GPCR rhodopsin in its native membrane by electron and atomic force microscopy (AFM). Disc membranes from mice were isolated and observed by AFM at room temperature. In all experimental conditions, rhodopsin forms structural dimers organized in paracrystalline arrays. A semi-empirical molecular model for the rhodopsin paracrystal is presented validating our previously reported results. Finally, we compare our model with other currently available models describing the supramolecular structure of GPCRs in the membrane.
Fotiadis
Dimitrios
D
M.E. Müller Institute for Microscopy, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.
Liang
Yan
Y
Department of Ophthalmology, University of Washington, Box 356485, Seattle, WA 98195-6485, USA.
Filipek
Slawomir
S
International Institute of Molecular and Cell Biology, PL-02109 Warsaw, Poland.
Saperstein
David A
DA
Department of Ophthalmology, University of Washington, Box 356485, Seattle, WA 98195-6485, USA.
Engel
Andreas
A
M.E. Müller Institute for Microscopy, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.
Palczewski
Krzysztof
K
Department of Ophthalmology, University of Washington, Box 356485, Seattle, WA 98195-6485, USA.|Department of Pharmacology, University of Washington, Seattle, WA 98195, USA.|Department of Chemistry, University of Washington, Seattle, WA 98195, USA.
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry*; D019281:Dimerization; D051379:Mice; D018625:Microscopy, Atomic Force; D008854:Microscopy, Electron; D008958:Models, Molecular; D020836:Protein Structure, Quaternary; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure*; D012243:Rhodopsin / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure*; D012374:Rod Cell Outer Segment / Q000648:ultrastructure
D043562:Receptors, G-Protein-Coupled; D012243:Rhodopsin