[Fingerprint construction of Panax notoginseng].
OBJECTIVE
To provide application and identification basis for Panax notoginseng by constructing the HPLC-FP of Panax Notoginsing.


METHODS
The luna C18(2) column was used with a mobile phase of (A) acetonitrile-(B) 0.05% phosphoric acid gradient elution, 0-8 min (20% A, 80% B), 8-20 min (20% A-40% A, 80% B-60% B), 20-30 min(40% A-20% A, 60% B-80% B), 30-36 min(20% A-100% A, 80% B-0), 36-50 min(100% A), 50-65 min (100% A-20% A, 0-80% B). The flow rate was 1.0 ml.min-1. The wavelength of detecter was set at 203 nm. Caffeine was internal standard.


RESULTS
By Cluster Analysis, the twenty-one notoginseng samples were classified as four clusters: the superior in producing area, the ordinary in producing area, the ordinary and the inferior. By Similarty Calculation, the similarity of the twenty-one notoginseng samples were 0.9-1.0, and the similarity of the eight common falses are less than 0.9.


CONCLUSIONS
The HPLC-FP of notoginseng has been established. The method can be used to identify and evaluate the quality of notoginseng.
Wang
Yan
Y
Panjin Institute of Drug Control, Panjin 124010, Liaoning, China.
Bi
Kai-shun
KS
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004340:Drug Contamination; D005894:Panax / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control