Detection of reactive oxygen and nitrogen species by EPR spin trapping.
Electron paramagnetic resonance spin trapping has become an indispensable tool for the specific detection of reactive oxygen free radicals in biological systems. In this review we describe some of the advantages as well as some experimental considerations of this technique and how it can be applied to biological systems to measure oxidative stress.
Villamena
Frederick A
FA
Center for Biomedical EPR Spectroscopy and Imaging, and The Davis Heart and Lung Research Institute, College of Medicine, The Ohio State University, Columbus, OH 43210, USA.
Zweier
Jay L
JL
D016477:Artifacts; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D005609:Free Radicals; D007700:Kinetics; D008055:Lipids / Q000737:chemistry; D008956:Models, Chemical; D018384:Oxidative Stress; D011110:Polymorphism, Genetic; D026361:Reactive Nitrogen Species / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013113:Spin Labels; D019097:Spin Trapping / Q000379:methods*
D005609:Free Radicals; D008055:Lipids; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D013113:Spin Labels