title: Optical microscopy of Arabidopsis seedlings fixed in non-fresh FAA using Kennedy Fixation Tubes.
abstract: Samples will be kept in non-fresh fixative for longer period than usual in Kennedy Space Center Fixation Tubes (KFT) when they will be fixed for microscopy in the International Space Station (ISS). It is necessary to examine characteristics of samples prepared under such conditions and to improve preservation of the samples. In this study, morphology of Arabidopsis tissues prepared under such conditions was examined under a light microscope. Deformation of cell shape was observed in tissues with well-developed intercellular spaces when they were fixed in non-fresh fixative for longer period in KFTs.
authors.affiliation: Department of Biology, Toyama University.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Honma
authors.forename: Izumi
authors.initials: I
authors.lastname: Nakabayashi
authors.forename: Daisuke
authors.initials: D
authors.lastname: Tamaoki
authors.forename: Haruo
authors.initials: H
authors.lastname: Kasahara
authors.forename: Noriaki
authors.initials: N
authors.lastname: Ishioka
authors.forename: Toru
authors.initials: T
authors.lastname: Shimazu
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kasahara
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Yamada
authors.forename: Ichirou
authors.initials: I
authors.lastname: Karahara
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Kamisaka
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology; D000438:Alcohols / Q000494:pharmacology; D017360:Arabidopsis / Q000166:cytology*; D048430:Cell Shape / Q000187:drug effects*; D005404:Fixatives / Q000494:pharmacology*; D005557:Formaldehyde / Q000494:pharmacology; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects; D036226:Seedlings / Q000166:cytology*; D013026:Space Flight / Q000295:instrumentation; D013048:Specimen Handling; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*
chemical_list: D000438:Alcohols; D005404:Fixatives; D005557:Formaldehyde; D019342:Acetic Acid