Synthesis and DNA binding properties of dioxime-peptide nucleic acids.
Peptide nucleic acids (PNAs) C- or N-modified with dioxime ligands were prepared by solid-phase synthesis using iron(II)-clathrochelates as protected dioxime building blocks. These PNA bind complementary DNA sequence specifically, though with much reduced affinity in comparison with nonmodified PNA. The dioxime-PNA conjugates bind Cu2+ and Ni2+ at microM concentration.
Mokhir
Andriy
A
Anorganisch-Chemisches Institut, Ruprecht-Karls-Universität Heidelberg, Im Neuenheimer Feld 270, 69120 Heidelberg, Germany. andriy.mokhir@urz.uni-heidelberg.de
Krämer
Roland
R
Voloshin
Yan Z
YZ
Varzatskii
Oleg A
OA
D001483:Base Sequence; D003300:Copper / Q000737:chemistry; D004247:DNA / Q000378:metabolism*; D015394:Molecular Structure; D009532:Nickel / Q000737:chemistry; D009691:Nucleic Acid Denaturation; D010091:Oximes / Q000138:chemical synthesis / Q000494:pharmacology; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013696:Temperature
D010091:Oximes; D020135:Peptide Nucleic Acids; D003300:Copper; D009532:Nickel; D004247:DNA