title: [The use of human fibroblasts grown on microcarriers for the formation of the connective tissue equivalent].
abstract: Live equivalents of tissues, specifically those produced on the basis of fibroblasts and collagen gel, are widely used for repair of organ and tissues defects. In clinical practice, it is more convenient to use the fibroblasts grown on microcarriers or such a connective tissue equivalent when the fibroblasts on microcarriers are embedded in collagen gel. We studied the properties of a connective tissue equivalent produced by embedding the fibroblasts grown on microcarriers in collagen gel for its prospective use in clinical practice. According to our results, the optimal time of use of the live tissue equivalent amounts to three--four days after embedding of fibroblasts on microcarriers in gel. At that time, contraction only begins, which facilitates manipulations with the gel.
authors.affiliation: Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 119991 Russia.
authors.forename: O S
authors.initials: OS
authors.lastname: Rogovaia
authors.forename: A V
authors.initials: AV
authors.lastname: Vasil'ev
authors.forename: I V
authors.initials: IV
authors.lastname: Kiselev
authors.forename: V V
authors.initials: VV
authors.lastname: Terskikh
mesh_terms: D001187:Artificial Organs; D001672:Biocompatible Materials; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D003094:Collagen; D003238:Connective Tissue; D004622:Embryo, Mammalian / Q000166:cytology; D004625:Embryo, Nonmammalian; D005347:Fibroblasts / Q000166:cytology*; D005782:Gels; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D005782:Gels; D003094:Collagen