title: [The first pregnancy and birth after cryopreservation of oocytes in the Czech Republic--importance of the method for reproductive medicine].
abstract: OBJECTIVE
  The purpose of this work was to develop methods for successful cryopreservation of human oocytes.
  
  
  METHODS
  Case-controlled study and case report.
  
  
  METHODS
  Sanatorium Pronatal, Prague.
  
  
  METHODS
  Propanediol (PrOH)-sucrose was used as cryoprotectant medium for cumulus-free oocytes and stepwise dilution of cryoprotectant post-thaw.
  
  
  RESULTS
  The method was used in three patients (38 denuded oocytes) and yielded excellent survival and fertilization rates (89.5% and 73.5% respectively). In all patients embryo-transfer was possible, one patient got pregnant and delivered a healthy baby.
  
  
  CONCLUSIONS
  Our data show that cryopreservation may ensure that the integrity of the human oocyte is adequate for normal fertilization and embryo development.
authors.affiliation: Sanatorium Pronatal, Praha.
authors.forename: R
authors.initials: R
authors.lastname: Huttelová
authors.forename: T
authors.initials: T
authors.lastname: Mardesić
authors.forename: V
authors.initials: V
authors.lastname: Becvárová
authors.forename: T
authors.initials: T
authors.lastname: Brachtlová
mesh_terms: D000328:Adult; D015925:Cryopreservation; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007248:Infertility, Male / Q000628:therapy; D008297:Male; D009865:Oocytes; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic