Modeling electron transfer thermodynamics in protein complexes: interaction between two cytochromes c(3).
Redox protein complexes between type I and type II tetraheme cytochromes c(3) from Desulfovibrio vulgaris Hildenborough are here analyzed using theoretical methodologies. Various complexes were generated using rigid-body docking techniques, and the two lowest energy complexes (1 and 2) were relaxed using molecular dynamics simulations with explicit solvent and subjected to further characterization. Complex 1 corresponds to an interaction between hemes I from both cytochromes c(3). Complex 2 corresponds to an interaction between the heme IV from type I and the heme I from type II cytochrome c(3). Binding free energy calculations using molecular mechanics, Poisson-Boltzmann, and surface accessibility methods show that complex 2 is more stable than complex 1. Thermodynamic calculations on complex 2 show that complex formation induces changes in the reduction potential of both cytochromes c(3), but the changes are larger in the type I cytochrome c(3) (the largest one occurring on heme IV, of approximately 80 mV). These changes are sufficient to invert the global titration curves of both cytochromes, generating directionally in electron transfer from type I to type II cytochrome c(3), a phenomenon of obvious thermodynamic origin and consequences, but also with kinetic implications. The existence of processes like this occurring at complex formation may constitute a natural design of efficient redox chains.
Teixeira
Vitor H
VH
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal.
Baptista
António M
AM
Soares
Cláudio M
CM
D001703:Biophysics / Q000379:methods; D018360:Crystallography, X-Ray; D003574:Cytochrome c Group / Q000737:chemistry* / Q000378:metabolism; D003580:Cytochromes / Q000737:chemistry; D016969:Desulfovibrio vulgaris / Q000378:metabolism*; D004579:Electron Transport; D004583:Electrons; D006418:Heme / Q000737:chemistry; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D010084:Oxidation-Reduction; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry; D011522:Protons; D013816:Thermodynamics; D013997:Time Factors; D014867:Water / Q000737:chemistry; D014965:X-Rays
D003574:Cytochrome c Group; D003580:Cytochromes; D011506:Proteins; D011522:Protons; D014867:Water; D006418:Heme; C017165:cytochrome c(3)