Amino acid sequence and function of a new alpha-toxin from the Amazonian scorpion Tityus cambridgei.
A toxic peptide earlier denominated Tc48b [Toxicon 40 (2002) 557] was purified to homogeneity and its amino acid sequence determined. It has 64 amino acid residues stabilized by four disulfide bridges with a molecular weight of 7,385.2 atomic mass units (a.m.u.). It affects Na(+)-permeability in pituitary GH3 cells in culture, in a similar fashion as those reported for alpha-scorpion toxins, contrary to most of the New World scorpion toxins that are beta-toxins.
Murgia
Anna Rosa
AR
Istituto di Biofisica, C.N.R., via De Marini 6, 16149 Genoa, Italy.
Batista
Cesar V F
CV
Prestipino
Gianfranco
G
Possani
Lourival D
LD
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000235:genetics* / Q000633:toxicity*; D010902:Pituitary Gland / Q000187:drug effects; D012604:Scorpion Venoms / Q000235:genetics* / Q000633:toxicity*; D015222:Sodium Channels / Q000187:drug effects*
D009498:Neurotoxins; D012604:Scorpion Venoms; D015222:Sodium Channels; C487692:toxin alpha, Tityus cambridgei