title: Polyamine metabolism in a member of the phylum Microspora (Encephalitozoon cuniculi): effects of polyamine analogues.
abstract: The uptake, biosynthesis and catabolism of polyamines in the microsporidian parasite Encephalitozoon cuniculi are detailed with reference to the effects of oligoamine and arylamine analogues of polyamines. Enc. cuniculi, an intracellular parasite of mammalian cells, has both biosynthetic and catabolic enzymes of polyamine metabolism, as demonstrated in cell-free extracts of mature spores. The uptake of polyamines was measured in immature, pre-emergent spores isolated from host cells by Percoll gradient. Spermine was rapidly taken up and metabolized to spermidine and an unknown, possibly acetamidopropanal, by spermidine/spermine N(1)-acetyltransferase (SSAT) and polyamine oxidase (PAO). Most of the spermidine and the unknown product were found in the cell incubation medium, indicating they were released from the cell. bis(Ethyl) oligoamine analogues of polyamines, such as SL-11144 and SL-11158, as well as arylamine analogues [BW-1, a bis(phenylbenzyl) 3-7-3 analogue] blocked uptake and interconversion of spermine at micromolar levels and, in the case of BW-1, acted as substrate for PAO. The Enc. cuniculi PAO activity differed from that found in mammalian cells with respect to pH optimum, substrate specificity and sensitivity to known PAO inhibitors. SL-11158 inhibited SSAT activity with a mixed type of inhibition in which the analogue had a 70-fold higher affinity for the enzyme than the natural substrate, spermine. The interest in Enc. cuniculi polyamine metabolism and the biochemical effects of these polyamine analogues is warranted since they cure model infections of Enc. cuniculi in mice and are potential candidates for human clinical trials.
authors.affiliation: Haskins Laboratories and Department of Biology, Pace University, New York, NY 10038, USA.
authors.forename: Cyrus J
authors.initials: CJ
authors.lastname: Bacchi
authors.affiliation: Haskins Laboratories and Department of Biology, Pace University, New York, NY 10038, USA.
authors.forename: Donna
authors.initials: D
authors.lastname: Rattendi
authors.affiliation: Haskins Laboratories and Department of Biology, Pace University, New York, NY 10038, USA.
authors.forename: Evangeline
authors.initials: E
authors.lastname: Faciane
authors.affiliation: Department of Chemistry, Pace University, New York, NY 10038, USA.|Haskins Laboratories and Department of Biology, Pace University, New York, NY 10038, USA.
authors.forename: Nigel
authors.initials: N
authors.lastname: Yarlett
authors.affiliation: Departments of Medicine and Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
authors.forename: Louis M
authors.initials: LM
authors.lastname: Weiss
authors.affiliation: SLIL Biomedical Corp., Madison, WI 53711, USA.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Frydman
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48202, USA.
authors.forename: Patrick
authors.initials: P
authors.lastname: Woster
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48202, USA.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Wei
authors.affiliation: SLIL Biomedical Corp., Madison, WI 53711, USA.
authors.forename: Laurence J
authors.initials: LJ
authors.lastname: Marton
authors.affiliation: Departments of Medicine and Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
authors.forename: Murray
authors.initials: M
authors.lastname: Wittner
mesh_terms: D000123:Acetyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism; D000818:Animals; D000981:Antiprotozoal Agents / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D009670:Encephalitozoon cuniculi / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000037:antagonists & inhibitors / Q000378:metabolism; D011073:Polyamines / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D011817:Rabbits; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D013379:Substrate Specificity; D000097667:Polyamine Oxidase; D000099228:Diamine N-Acetyltransferase
chemical_list: D000123:Acetyltransferases; D000981:Antiprotozoal Agents; D004791:Enzyme Inhibitors; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011073:Polyamines; D013095:Spermidine; D013096:Spermine; D000097667:Polyamine Oxidase; D000099228:Diamine N-Acetyltransferase; C465646:SL-11158