Involvement of protein kinases on the process of erythrophagocytis by Entamoeba histolytica.
Erythrophagocytic capacity of trophozoites of Entamoeba histolytica is considered a factor in the virulence of this pathogenic protozoan. We present evidence showing that such activity resembles the ingestion of microorganisms by highly differentiated phagocytic cells, such as macrophages. Previous treatment of the trophozoites with genistein or tyrphostin, inhibitors of tyrosine protein kinases, with staurosporine, a protein kinase C inhibitor, and wortmannin, a fungal metabolite that inhibits phosphoinositide 3-OH kinase, significantly inhibited their erythrophagocytic capacity.
Batista
Evander de J O
Ede J
Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCS-Bloco G, Ilha do Fundão, Rio de Janeiro RJ, 21941-900, Brazil.
de Souza
Wanderley
W
D000730:Androstadienes / Q000494:pharmacology; D000818:Animals; D004748:Entamoeba histolytica / Q000201:enzymology* / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004912:Erythrocytes / Q000502:physiology*; D019833:Genistein / Q000494:pharmacology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology*; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism*; D019311:Staurosporine / Q000494:pharmacology; D020032:Tyrphostins / Q000494:pharmacology; D000077191:Wortmannin
D000730:Androstadienes; D004791:Enzyme Inhibitors; D047428:Protein Kinase Inhibitors; D020032:Tyrphostins; D019833:Genistein; D011494:Protein Kinases; D019311:Staurosporine; D000077191:Wortmannin