title: Differential distribution of MAP1A isoforms in the adult mouse barrel cortex and comparison with the serotonin 5-HT2A receptor.
abstract: Microtubule-associated protein 1A (MAP1A) is essential during the late differentiation phase of neuronal development. Here, we demonstrated the presence of two MAP1A isoforms with a differential spatial distribution in the adult mouse barrel cortex. Antibody A stained MAP1A in pyramidal and stellate cells, including dendrites that crossed layer IV in the septa between barrels. The other antibody, BW6 recognized a MAP1A isoform that was mainly confined to the barrel hollow and identified smaller caliber dendrites. Previously, an interaction of MAP1A and the serotonin 5-hydroxytryptamine 2A (5-HT(2A)) receptor was shown in the rat cortex. Here, we identified, by double-immunofluorescent labeling, MAP1A isoform and serotonin 5-HT(2A) receptor distribution. MAP1A co-localized mainly with 5-HT(2A) receptor in larger apical dendrites situated in septa. This differential staining of MAP1A and a serotonin receptor in defined barrel compartments may be due to changes in the expression or processing of MAP1A during dendritic transport as a consequence of functional differences in processing of whisker-related sensory input.
authors.affiliation: Institut de Biologie Cellulaire et de Morphologie, Université de Lausanne, Rue du Bugnon 9, 1005 Lausanne, Switzerland.
authors.forename: F
authors.initials: F
authors.lastname: Touri
authors.forename: E
authors.initials: E
authors.lastname: Welker
authors.forename: B M
authors.initials: BM
authors.lastname: Riederer
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000033:anatomy & histology* / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D051379:Mice; D018613:Microscopy, Confocal; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D044402:Receptor, Serotonin, 5-HT2A / Q000378:metabolism
chemical_list: C490547:MAP1A protein, mouse; C490546:Map1a protein, rat; D008869:Microtubule-Associated Proteins; D020033:Protein Isoforms; D044402:Receptor, Serotonin, 5-HT2A