Transglycosylation reaction of Mucor hiemalis endo-beta-N-acetylglucosaminidase using sugar derivatives modified at C-1 or C-2 as oligosaccharide acceptors.
We investigated the transglycosylation reaction of the recombinant endo-beta-N-acetylglucosaminidase from Mucor hiemalis (Endo-M) expressed in Candida boidinii using such sugar derivatives as N-acylated d-glucosamines, C-glucosyl derivatives, and a 2-O-glycosylated disaccharide as acceptors. We found that a variety of sugar derivatives modified at C-1 or C-2 could be used as acceptors for transglycosylation by Endo-M to create novel oligosaccharides.
Yamanoi
Takashi
T
The Noguchi Institute, 1-8-1 Kaga, Itabashi-ku, Tokyo 173-0003, Japan. tyama@noguchi.or.jp
Tsutsumida
Maki
M
Oda
Yoshiki
Y
Akaike
Eri
E
Osumi
Kenji
K
Yamamoto
Kenji
K
Fujita
Kiyotaka
K
D000117:Acetylglucosamine / Q000031:analogs & derivatives / Q000737:chemistry; D004187:Disaccharides / Q000737:chemistry; D006031:Glycosylation; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase / Q000737:chemistry*; D015394:Molecular Structure; D009089:Mucor / Q000201:enzymology*; D009844:Oligosaccharides / Q000737:chemistry*
D004187:Disaccharides; D009844:Oligosaccharides; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D000117:Acetylglucosamine