title: Development of a monoclonal immunoassay selective for chlorinated cyclodiene insecticides.
abstract: Organochlorine pesticides still generate public health concerns because of their unresolved health impact and their persistence in living beings, which is demanding appropriate analytical techniques for their monitoring. In this study, an enzyme-linked immunosorbent assay based on monoclonal antibodies (MAbs) for the detection of an important group of organochlorine pesticides, the cyclodiene group, has been developed. With this aim, several hapten-protein conjugates, characterized by exposure of the common hexachlorinated bicyclic (norbornene) moiety and differing in the linking structure to the carrier protein, were prepared. From mice immunized with these conjugates, several MAbs with the ability to sensitively bind the majority of cyclodienes were obtained. Among them CCD2.2 MAb displaying the broadest recognition to cyclodiene compounds (endosulfan, dieldrin, endrin, chlordane, heptachlor, aldrin, toxaphene: I(50) values in the 6-25 nM range) was selected for the assay. Interestingly, this MAb showed certain stereospecificity toward other polychlorinated cycloalkanes because the gamma-isomer of hexachlorocyclohexane (lindane) was also very well recognized (I(50) value of 22 nM). This immunoassay is potentially a very valuable analytical tool for the rapid and sensitive determination of cyclodiene insecticides and related compounds, which in turn may contribute to the understanding of the biological activities and of the overall environmental impact of these persistent organic pollutants.
authors.affiliation: Centro de Investigación e Innovación en Bioingeniería and Instituto de Tecnología Química, Universidad Politécnica de Valencia, 46022 Valencia, Spain.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Manclús
authors.forename: Antonio
authors.initials: A
authors.lastname: Abad
authors.forename: Mijail Y
authors.initials: MY
authors.lastname: Lebedev
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mojarrad
authors.forename: Barbora
authors.initials: B
authors.lastname: Micková
authors.forename: Josep V
authors.initials: JV
authors.lastname: Mercader
authors.forename: Jaime
authors.initials: J
authors.lastname: Primo
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Miranda
authors.forename: Angel
authors.initials: A
authors.lastname: Montoya
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000918:Antibody Specificity; D006241:Haptens; D006843:Hydrocarbons, Chlorinated; D007118:Immunoassay / Q000379:methods*; D007306:Insecticides / Q000032:analysis*; D051379:Mice; D012997:Solvents
chemical_list: D000911:Antibodies, Monoclonal; D006241:Haptens; D006843:Hydrocarbons, Chlorinated; D007306:Insecticides; D012997:Solvents