Plasma intestinal fatty acid binding protein (I-FABP) concentrations increase following intestinal ischemia in pigs.
Intestinal fatty acid binding protein (I-FABP) is an intracellular epithelial protein in the intestinal mucosa of many animals. I-FABP appears in the circulation following epithelial damage, and in humans, is proven to be a parameter for damage to the mucosa. In this paper, an ELISA test designed for human I-FABP analysis was used to assay pig blood samples. The test recognized I-FABP cloned from pig small intestine and expressed in Escherichia coli. Furthermore, in our experimental model of (low flow) intestinal ischemia and reperfusion a significant rise in plasma I-FABP concentrations 15-30 min after clamping of the mesenteric artery was demonstrated. This is the first report that in pigs circulating I-FABP is a useful marker for (mild) intestinal injury, and could possibly be used to monitor (intestinal) health in clinical practice.
Niewold
T A
TA
Animal Sciences Group, Division of Animal Resources Development, Research Group Animal Physiology and Health, P.O. Box 65, 8200 AB Lelystad, The Netherlands. theo.niewold@wur.nl
Meinen
M
M
van der Meulen
J
J
D000818:Animals; D002352:Carrier Proteins / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D050556:Fatty Acid-Binding Proteins; D007413:Intestinal Mucosa / Q000098:blood supply*; D008297:Male; D017538:Mesenteric Artery, Superior; D015427:Reperfusion Injury / Q000097:blood / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000097:blood*
D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins