Analysis of liposomal membrane composition using Raman tweezers.
We have developed a methodology for the analysis of liposomal membranes and their contents using near-IR Raman spectroscopy on liposomes held in an optical trap. We were able to detect a variety of membrane components including lipids, cholesterol, and small molecule solutes such as ethanol, DMSO and hexafluoroisopropanol. The methodology is able to distinguish between solutes that equilibrate across the liposomal membrane from those that partition selectively into the lipid bilayer.
Sanderson
John M
JM
University Science Laboratories, Department of Chemistry, South Road, Durham, UK DH1 3LE. j.m.sanderson@dur.ac.uk
Ward
Andrew D
AD
D002784:Cholesterol / Q000032:analysis / Q000737:chemistry; D004121:Dimethyl Sulfoxide / Q000032:analysis / Q000737:chemistry; D000431:Ethanol / Q000032:analysis / Q000737:chemistry; D008055:Lipids / Q000032:analysis / Q000737:chemistry; D008081:Liposomes / Q000032:analysis / Q000737:chemistry*; D020005:Propanols / Q000032:analysis / Q000737:chemistry; D012995:Solubility; D013055:Spectrophotometry, Infrared; D013059:Spectrum Analysis, Raman / Q000379:methods*; D006845:Hydrocarbons, Fluorinated
D002784:Cholesterol; D004121:Dimethyl Sulfoxide; D000431:Ethanol; D008055:Lipids; D008081:Liposomes; D020005:Propanols; C001337:hexafluoroisopropanol; D006845:Hydrocarbons, Fluorinated