title: Scale-up micropropagation of sweet basil (Ocimum basilicum L.) in an airlift bioreactor and accumulation of rosmarinic acid.
abstract: Nodal explants with lateral buds and leaf-derived suspension cultures of sweet basil, Ocimum basilicum L., were cultured in 5 l airlift bioreactors for three weeks, thereby increasing the fresh wt of suspension cultures 2.5-fold. Rosmarinic acid accumulated at 29 micromicrog g(-1) dry wt in the suspension culture but, for micropropagated plants, it reached 178 microg g(-1) dry wt.
authors.affiliation: Laboratory of Plant Physiology, Faculty of Agricultural Biotechnology Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece. skin@aua.gr
authors.forename: Spiridon
authors.initials: S
authors.lastname: Kintzios
authors.forename: Haralambos
authors.initials: H
authors.lastname: Kollias
authors.forename: Evangelos
authors.initials: E
authors.lastname: Straitouris
authors.forename: Olga
authors.initials: O
authors.lastname: Makri
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D019149:Bioreactors; D002934:Cinnamates / Q000378:metabolism*; D053630:Depsides; D018649:Ocimum basilicum / Q000378:metabolism*; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism*; D000097123:Rosmarinic Acid
chemical_list: D000975:Antioxidants; D002934:Cinnamates; D053630:Depsides; D000097123:Rosmarinic Acid