title: Degradation pathways of cyclic alkanes in Rhodococcus sp. NDKK48.
abstract: The degradation pathways for cyclic alkanes (c-alkanes) in Rhodococcus sp. NDKK48 were investigated. Strain NDKK48 used dodecylcyclohexane as a sole carbon and energy source, and five metabolites in the dodecylcyclohexane degradation pathway were detected by gas-chromatography/mass spectra. The metabolites were identified as cyclohexanecarboxylic acid, cyclohexylacetic acid, 1-cyclohexene-1-acetic acid, 4-dodecylcyclohexanol, and 4-dodecylcyclohexanone. The strain degrades dodecylcyclohexane via a ring oxidation pathway and an alkyl side chain oxidation pathway. Cyclohexanecarboxylic acid was further oxidized to muconic acid via 1-cyclohexene-1-carboxylic acid and benzoic acid, and the muconic acid was finally used by strain NDKK48 for growth. Methylcyclohexane and cyclohexane were co-oxidized with hexadecane by strain NDKK48. Methylcyclohexane was degraded via a ring oxidation pathway, and the degradation pathway contained part of the Baeyer-Villiger oxidation for ring cleavage. Cyclohexane was also degraded by the same pathway as methylcyclohexane. Thus, strain NDKK48 has two pathways for the complete degradation of c-alkanes.
authors.affiliation: Department of Bioscience and Technology, Faculty of Science and Engineering, Ritsumeikan University, Kusatsu, 525-8577 Shiga, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Koma
authors.forename: Y
authors.initials: Y
authors.lastname: Sakashita
authors.forename: K
authors.initials: K
authors.lastname: Kubota
authors.forename: Y
authors.initials: Y
authors.lastname: Fujii
authors.forename: F
authors.initials: F
authors.lastname: Hasumi
authors.forename: S Y
authors.initials: SY
authors.lastname: Chung
authors.forename: M
authors.initials: M
authors.lastname: Kubo
mesh_terms: D001673:Biodegradation, Environmental; D003510:Cyclohexanes / Q000737:chemistry / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D015394:Molecular Structure; D012240:Rhodococcus / Q000378:metabolism*; D013997:Time Factors
chemical_list: D003510:Cyclohexanes