title: Tyrosine-induced melanogenesis shows differences in morphologic and melanogenic preferences of melanosomes from light and dark skin types.
abstract: The quality, quantity and distribution of melanosomes in epidermis play a crucial role in the determination of skin color and its sensitivity to UV radiation. Melanocyte cultures originating from individuals with light and dark skin types were grown in media with varying concentration of L-tyrosine. Melanosomal melanin content and the size of the organelles were measured after subcellular fractionation. In light-skin type cells, increased melanin production resulted in a more elliptical shape of melanosomes. In melanosomes that constitutively produce more melanin, the tyrosine-induced melanogenesis caused enlargement in all dimensions. X-ray microanalysis provided evidence that the increase in sulfur content induced by high tyrosine concentration was more prominent in the melanosomes from light skin types. A ratio between pheomelanin and eumelanin found in light-skin type melanosomes by HPLC was increased more markedly than that in melanosomes from dark skin melanocytes. These findings suggest that the melanocytes of light-skinned individuals exhibit a preference for pheomelanogenesis. Pheomelanin production is a thiol-consuming process and that might increase the risk of oxidation stress in these cells. This fact, together with the limited ability of pheomelanin to absorb UV radiation may lead to an elevated skin cancer risk among light-skinned individuals.
authors.affiliation: Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands. s.pavel@lumc.nl
authors.forename: Frans
authors.initials: F
authors.lastname: van Nieuwpoort
authors.forename: Nico P M
authors.initials: NP
authors.lastname: Smit
authors.forename: Ria
authors.initials: R
authors.lastname: Kolb
authors.forename: Hans
authors.initials: H
authors.lastname: van der Meulen
authors.forename: Henk
authors.initials: H
authors.lastname: Koerten
authors.forename: Stan
authors.initials: S
authors.lastname: Pavel
mesh_terms: D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000494:pharmacology; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism*; D006801:Humans; D008543:Melanins / Q000096:biosynthesis; D020460:Melanosomes / Q000187:drug effects / Q000378:metabolism*; D012880:Skin Pigmentation / Q000502:physiology*; D013455:Sulfur / Q000378:metabolism; D014443:Tyrosine / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D008543:Melanins; C018362:pheomelanin; C041877:eumelanin; D014443:Tyrosine; D013455:Sulfur