Stability indicating method for SPf66 antimalarial peptide in solution.
Stability studies on the SPf66 antimalarial peptide with different pH and temperature conditions were carried out. The degradation mechanism was elucidated by the size-exclusion chromatography (SEC) technique and the experimental data obtained at 37 degrees C and different pH were fitted to a kinetic degradation model that could explain the loss of its immunogenic capacity. At 5, 25, 37, and 70 degrees C and pH 2, changes were detected in the areas of the different species, although the values obtained could not be fitted to any known degradation kinetics.
Santoveña
A
A
Dpto. Ingeniería Química y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de La Laguna, Tenerife, Spain.
Dorta
M J
MJ
Oliva
A
A
Llabrés
M
M
Patarroyo
M E
ME
Fariña
J B
JB
D002850:Chromatography, Gel; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D017780:Malaria Vaccines / Q000737:chemistry*; D015800:Protozoan Proteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry*; D013696:Temperature; D014614:Vaccines, Synthetic / Q000737:chemistry
D017780:Malaria Vaccines; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C066269:SPf66 protein, Plasmodium; D014614:Vaccines, Synthetic