Expression of allergens in E. coli and plants--benefits and drawbacks.
Recombinant allergens are quickly becoming the reagents of choice for diagnosis and therapy of type I allergic diseases. Consequently, the different methods for the production of recombinant proteins that are available today are of great interest to allergologists. Without doubt, bacterial expression will continue to play a pivotal role. In addition, plant-based expression systems will be needed to overcome problems inherent in the E. coli systems and to allow the production of glycoallergens or allergens of more complex folding.
Breiteneder
Heimo
H
Dept. of Pathophysiology, University of Vienna, Währinger Gürtel 18-20, A-1090 Vienna.
Wagner
Birgit
B
D000485:Allergens / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D015870:Gene Expression; D010944:Plants / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D000485:Allergens; D011994:Recombinant Proteins