Abscisic acid and jasmonic acid affect proteinase inhibitor activities in barley leaves.
Proteinase inhibitor (PI) accumulation has been described as a plant defense response against insects and pathogens. The induction of PIs is known to be regulated by endogenous chemical factors including phytohormones. We studied the induction of barley chymotrypsin and trypsin inhibitory activities by aphid infestation, mechanical wounding, abscisic acid (ABA) and jasmonic acid (JA). Wounding experiments led to a minimal accumulation of PI activity (16% over controls) compared to that found in barley seedlings infested by aphids, where chymotrypsin inhibitor activity showed a two-fold increment. No systemic induction could be detected in healthy leaves of an infested or mechanically injured plant. Exogenous ABA applied on barley leaves increased the chymotrypsin inhibitory activity, while JA only increased trypsin inhibitory activity locally and systemically when applied exogenously. Our data suggest that two different mechanisms may be regulating the induction of these two types of inhibitors.
Casaretto
José A
JA
Departamento de Biología, Universidad de Chile, Casilla 653, Santiago, Chile. casarett@biology.wustl.edu
Zúñiga
Gustavo E
GE
Corcuera
Luis J
LJ
D000040:Abscisic Acid / Q000494:pharmacology*; D000818:Animals; D001042:Aphids / Q000254:growth & development; D002918:Chymotrypsin / Q000378:metabolism; D003517:Cyclopentanes / Q000494:pharmacology*; D001467:Hordeum / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D007113:Immunity, Innate / Q000502:physiology; D054883:Oxylipins; D010935:Plant Diseases / Q000469:parasitology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects* / Q000378:metabolism / Q000469:parasitology; D011480:Protease Inhibitors / Q000378:metabolism*; D013314:Stress, Mechanical; D014357:Trypsin / Q000378:metabolism
D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D011480:Protease Inhibitors; C011006:jasmonic acid; D000040:Abscisic Acid; D002918:Chymotrypsin; D014357:Trypsin