title: Comparative study of anti-double stranded DNA detection by ELISA and Crithidia luciliae immunofluorescence.
abstract: Two commercial enzyme-linked immunosorbent assays (ELISA) and two commercial Crithidia luciliae immunofluorescence tests (CLIF) were reevaluated as to the efficiency and degree of correlation of anti-double stranded DNA (anti-dsDNA) detection in systemic lupus erythematosus (SLE). The two ELISAs exhibited an overall agreement of 95% and significantly correlated with each other (r=0.91, p<0.001). They were comparable in sensitivity (64%, 61%) and had the same specificity (95%, 95%). The sensitivity of the two CLIFs was 39% and 29% with corresponding specificities of 100% and 97%, and an overall agreement with each other of 94%. The two ELISAs had comparable specificity to the CLIFs with good agreement (84%, 79%) while they had a much greater sensitivity than the CLIFs. These findings suggest that ELISA is a useful laboratory test for anti-dsDNA detection of SLE due to its simplicity, quantitative results, sensitivity, specificity and cost, as compared to CLIFs.
authors.affiliation: Faculty of Medical Technology, Rangsit University, Pathum Thani, Thailand. janya@hotmail.com
authors.forename: Kritsana
authors.initials: K
authors.lastname: Janyapoon
authors.forename: Pantakit
authors.initials: P
authors.lastname: Jivakanont
authors.forename: Kantima
authors.initials: K
authors.lastname: Choosang
authors.forename: Rungkarn
authors.initials: R
authors.lastname: Surbrsing
authors.forename: Vannapa
authors.initials: V
authors.lastname: Charoenying
authors.forename: Sirinya
authors.initials: S
authors.lastname: Baithong
mesh_terms: D000974:Antibodies, Antinuclear / Q000032:analysis*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D016014:Linear Models; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis* / Q000235:genetics; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D000974:Antibodies, Antinuclear; D011933:Reagent Kits, Diagnostic