Isolation and identification of two isomeric forms of malonyl-coenzyme A in commercial malonyl-coenzyme A.
Two isomers of malonyl-coenzyme A (malonyl-CoA) were detected in a commercial preparation of malonyl-CoA. These compounds were separated by preparative high-performance liquid chromatography (HPLC) and characterized by HPLC/ultraviolet (UV)/mass spectrometry. Both compounds had a UV absorbance maximum at 259-260 nm. Both compounds underwent negative electrospray ionization to produce a [M-H](-)quasi-molecular ion at m/z 852 and both compounds underwent collision-induced dissociation to produce a characteristic fragment at m/z 808, all consistent with the structure of malonyl-CoA. Nuclear magnetic resonance spectrometry showed that the two chromatographically distinguishable malonyl-CoAs are structural isomers: the major component is the naturally occurring malonyl-CoA and the contaminant is 3'-dephospho- 2'-phospho-coenzyme A.
Minkler
Paul E
PE
Medical Research Service, Louis Stokes Department of Veterans Affairs Medical Center, Cleveland, OH 44106, USA.
Anderson
Vernon E
VE
Maiti
Nakul C
NC
Kerner
Janos
J
Hoppel
Charles L
CL
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003065:Coenzyme A / Q000737:chemistry / Q000302:isolation & purification; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008316:Malonyl Coenzyme A / Q000737:chemistry / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D008316:Malonyl Coenzyme A; D003065:Coenzyme A