title: An all-in-one dry chemistry immunoassay for the screening of coccidiostat nicarbazin in poultry eggs and liver.
abstract: An automated immunoassay for the detection of nicarbazin residues in poultry eggs and liver was developed. The assay was based on a novel all-in-one dry chemistry concept and time-resolved fluorometry. The analyte specific antibody was immobilized into a single microtiter well and covered with an insulation layer, on top of which the label was dried in a small volume. The extracted sample was added automatically to the dry microtiter well, and the result was available within 18 min. Due to the rapidity and simplicity, the quantitative immunoassay could also be used as a high throughput screening method. The analytical limit of detection for the assay was calculated as 0.1 ng mL(-)(1) (n = 12) and the functional limit of detection as 3.2 ng g(-)(1) for egg (n = 6) and 11.3 ng g(-)(1) for liver (n = 6) samples. The sample recovery varied from 97.3 to 115.6%. Typically, the intra-assay variations were less than 10%, and interassay variations ranged between 8.1 and 13.6%.
authors.affiliation: Department of Biotechnology, University of Turku, Tykistökatu 6A, FIN-20520 Turku, Finland. virve.hagren@utu.fi
authors.forename: Virve
authors.initials: V
authors.lastname: Hagren
authors.forename: Steven R H
authors.initials: SR
authors.lastname: Crooks
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Elliott
authors.forename: Timo
authors.initials: T
authors.lastname: Lövgren
authors.forename: Mika
authors.initials: M
authors.lastname: Tuomola
mesh_terms: D000818:Animals; D002645:Chickens; D003049:Coccidiostats / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis; D004531:Eggs / Q000032:analysis*; D015200:Fluoroimmunoassay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D008099:Liver / Q000737:chemistry*; D009528:Nicarbazin / Q000032:analysis*
chemical_list: D003049:Coccidiostats; D009528:Nicarbazin