Feasibility of polysaccharide hybrid materials for scaffolds in cartilage tissue engineering: evaluation of chondrocyte adhesion to polyion complex fibers prepared from alginate and chitosan.
The ideal cell-carrier material for cartilage regeneration should be one that closely mimics the natural environment in a living articular cartilage matrix. In the current study, we considered that alginate-based chitosan hybrid biomaterials could provide excellent supports for chondrocyte adhesion. To test this hypothesis, we investigated the adhesion behavior of rabbit chondrocytes onto an alginate polymer versus the adhesion of the chondrocytes onto some alginate-based chitosan hybrid polymer fibers in vitro. We demonstrated that the alginate-based chitosan hybrid polymer fibers showed much improved adhesion capacity with chondrocytes in comparison with alginate polymer fiber. Additionally, morphologic studies revealed maintenance of the characteristic round morphology of the chondrocyte and the dense fiber of the type II collagen produced by the chondrocytes in the hybrid polymer. On the basis of these results, we conclude that an alginate-based chitosan hybrid polymer fiber has considerable potential as a desirable biomaterial for cartilage tissue scaffolds.
Iwasaki
Norimasa
N
Department of Orthopaedic Surgery, Hokkaido University School of Medicine, Sapporo 060-8638, Japan. niwasaki@med.hokudai.ac.jp
Yamane
Shin-Tarou
ST
Majima
Tokifumi
T
Kasahara
Yasuhiko
Y
Minami
Akio
A
Harada
Kazuo
K
Nonaka
Sachiko
S
Maekawa
Nobuhiko
N
Tamura
Hiroshi
H
Tokura
Seiichi
S
Shiono
Masamichi
M
Monde
Kenji
K
Nishimura
Shin-ichiro
S
D000464:Alginates / Q000737:chemistry*; D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology*; D002448:Cell Adhesion; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry*; D019902:Chondrocytes / Q000166:cytology*; D004247:DNA / Q000032:analysis; D005240:Feasibility Studies; D020723:Glucuronic Acid / Q000737:chemistry*; D006603:Hexuronic Acids / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D011134:Polysaccharides / Q000737:chemistry*; D011817:Rabbits; D023822:Tissue Engineering
D000464:Alginates; D006603:Hexuronic Acids; D011134:Polysaccharides; D020723:Glucuronic Acid; D004247:DNA; D048271:Chitosan