title: [Evaluation of experimental cartilage lesions with ultrahigh-resolution multi-slice-CT in comparison to histology].
abstract: OBJECTIVE
  Histologic validation of ultrahigh-resolution multi-slice (MS)-CT for the evaluation of focal, experimental cartilage lesions with special regard to the subchondral bone. Testing of micro-CT ( micro CT) as alternative reference standard.
  
  
  METHODS
  32 experimental cartilage lesions in bovine patellae were imaged surrounded by air (MS-CT-air) and immersed in a contrast material solution (MS-CT-CM) with MS-CT (collimation 2 x 0,5 mm). After the micro CT (8 micro m-voxelsite) examination in three specimen and histologic work-up of 29 specimen two radiologist graded the defects on MS-CT images in consensus (subchondral bone involvement yes or no) and results were compared to the results of histomorphometry and micro CT.
  
  
  RESULTS
  The MS-CT-air and -CM had an accuracy of 94 % (30/32) and 88 % (28/32), respectively. MS-CT-air led to one false-positive (remaining cartilage: = 0,1 mm) and false-negative result, each. MS-CT-CM showed false-positive results if the remaining cartilage was < 0,3 mm thick (n = 4), i. e. showed subchondral bone involvement. Contrast-to-noise ratio was significantly higher in MS-CT-air compared to MS-CT-CM. micro CT yielded a clear depiction of cartilage defect depth in the three cases.
  
  
  CONCLUSIONS
  MS-CT-air has a high accuracy in the depiction of focal cartilage defects. MS-CT-CM has a tendency to overestimate cartilage defect depth. micro CT could potentially serve as alternative reference standard to histology.
authors.affiliation: Klinik für Diagnostische und Interventionelle Radiologie. astork@uke.uni-hamburg.de
authors.forename: A
authors.initials: A
authors.lastname: Stork
authors.forename: J
authors.initials: J
authors.lastname: Kemper
authors.forename: M
authors.initials: M
authors.lastname: Priemel
authors.forename: T
authors.initials: T
authors.lastname: Kummer
authors.forename: P G
authors.initials: PG
authors.lastname: Begemann
authors.forename: C R
authors.initials: CR
authors.lastname: Habermann
authors.forename: M
authors.initials: M
authors.lastname: Amling
authors.forename: G
authors.initials: G
authors.lastname: Adam
mesh_terms: D000818:Animals; D002357:Cartilage Diseases / Q000000981:diagnostic imaging* / Q000473:pathology*; D002358:Cartilage, Articular / Q000033:anatomy & histology / Q000000981:diagnostic imaging* / Q000293:injuries* / Q000473:pathology; D002417:Cattle; D003287:Contrast Media; D006652:Histological Techniques; D066298:In Vitro Techniques; D010329:Patella; D012015:Reference Standards; D036542:Tomography, Spiral Computed; D014057:Tomography, X-Ray Computed / Q000379:methods*
chemical_list: D003287:Contrast Media