title: Overexpression of DRG2 increases G2/M phase cells and decreases sensitivity to nocodazole-induced apoptosis.
abstract: DRG2, a member of the DRG subfamily in the GTP-binding protein superfamily, was identified as a repressed gene product in fibroblasts transformed by SV40. The significance of this down-regulation and the cellular role of DRG2 has not been understood in the past. To investigate the function of DRG2 we made a Jurkat cell line, Jurkat-LNCX2-DRG2, stably transfected with pLNCX2-DRG2 to overexpress human DRG2. Cell cycle distribution analysis revealed an increased accumulation of G(2)/M phase cells in Jurkat-LNCX2-DRG2 cells, indicating a retardation of cell-cycle progression. In addition, an overexpression of DRG2 reduced the sensitivity of Jurkat cells to the mitotic poison nocodazole. Our data suggest that overexpression of DRG2 in Jurkat cells affects genes regulating cell-cycle arrest and apoptosis, and that these molecular changes may be important in the growth or differentiation of cells.
authors.affiliation: Immunomodulation Research Center, University of Ulsan, Ulsan 680-749, Korea.
authors.forename: Hebok
authors.initials: H
authors.lastname: Song
authors.forename: Sin-Il
authors.initials: SI
authors.lastname: Kim
authors.forename: Myoung Seok
authors.initials: MS
authors.lastname: Ko
authors.forename: Hyo Jeong
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jin Chul
authors.initials: JC
authors.lastname: Heo
authors.forename: Hae Jin
authors.initials: HJ
authors.lastname: Lee
authors.forename: Han Saem
authors.initials: HS
authors.lastname: Lee
authors.forename: In Seob
authors.initials: IS
authors.lastname: Han
authors.forename: KyuBum
authors.initials: K
authors.lastname: Kwack
authors.forename: Jeong Woo
authors.initials: JW
authors.lastname: Park
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D016195:G2 Phase / Q000187:drug effects; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D015739:Nocodazole / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: C409877:DRG2 protein, human; D012333:RNA, Messenger; D019204:GTP-Binding Proteins; D015739:Nocodazole