title: Amperometric detection of glucose using self-catalytic carbon paste electrodes.
abstract: The analytical detection of d-glucose by means of self-catalytic carbon paste electrodes is described. In the construction of these electrodes, carbon powder has been modified with the redox liquid n-butylferrocene, which simultaneously serves both to help bind the paste together whilst also acting as a mediator in the enzymatic oxidation of d-glucose by glucose oxidase. The sensor then functions by monitoring the electrochemical oxidation of the constituent n-butylferrocene itself. Through testing in model glucose solutions, the electrodes were found to yield a linear response over a d-glucose concentration range of 2-20 mM. They were also successfully employed in the determination of d-glucose levels in a spiked blood sample, giving a detection limit of 0.8 mM (based on the 3sigma criterion).
authors.affiliation: Physical and Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, UK.
authors.forename: Russell G
authors.initials: RG
authors.lastname: Evans
authors.forename: Craig E
authors.initials: CE
authors.lastname: Banks
authors.forename: Richard G
authors.initials: RG
authors.lastname: Compton
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000032:analysis*; D002244:Carbon; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D006736:Horses
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D002244:Carbon