title: PADMA 28 modulates interferon-gamma-induced tryptophan degradation and neopterin production in human PBMC in vitro.
abstract: Tibetan herbal remedy PADMA 28 revealed promising results to support treatment of intermittent claudication, atherosclerosis and chronic hepatitis. The remedy was confirmed to be closely linked with anti- and pro-oxidative properties in vitro. In this study, effect of PADMA 28 was investigated in stimulated and unstimulated human peripheral blood mononuclear cells (PBMC) in vitro. Neopterin production and tryptophan degradation were measured in supernatants of PBMC in the presence or absence of mitogens phytohaemagglutinin (PHA) and concanavalin A (Con A). Stimulation of PBMC induced neopterin formation and tryptophan degradation (p<0.001 compared to unstimulated PBMC), and PADMA 28 inhibited both immunobiochemical effects (p<0.001) in a concentration-dependent manner. Higher concentrations of PADMA 28 were more effective and were able to completely block the pathways induced upon mitogenic stimulation. Data allow to conclude that PADMA 28 is able to inhibit immunobiological effects in stimulated PBMC in vitro. The suppression of neopterin production and tryptophan degradation suggests a specific influence on biochemical pathways induced by Th1-type cytokine interferon-gamma.
authors.affiliation: Institute of Medical Chemistry and Biochemistry, Leopold Franzens University, Innsbruck, Austria.
authors.forename: G
authors.initials: G
authors.lastname: Neurauter
authors.forename: B
authors.initials: B
authors.lastname: Wirleitner
authors.forename: K
authors.initials: K
authors.lastname: Schroecksnadel
authors.forename: H
authors.initials: H
authors.lastname: Schennach
authors.forename: F
authors.initials: F
authors.lastname: Ueberall
authors.forename: D
authors.initials: D
authors.lastname: Fuchs
mesh_terms: D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007502:Iron Chelating Agents / Q000494:pharmacology*; D007737:Kynurenine / Q000378:metabolism; D008934:Mitogens / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D019798:Neopterin / Q000096:biosynthesis*; D010835:Phytohemagglutinins / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D012997:Solvents; D014364:Tryptophan / Q000378:metabolism*
chemical_list: D007502:Iron Chelating Agents; D008934:Mitogens; D010835:Phytohemagglutinins; D010936:Plant Extracts; D012997:Solvents; D003208:Concanavalin A; C066069:Padma 28; D007737:Kynurenine; D000431:Ethanol; D019798:Neopterin; D007371:Interferon-gamma; D014364:Tryptophan