[Analysis of DNA markers specific for G-genome of wheat].
A RAPD marker specific for the G genome of wheat was identified. The corresponding 1171-bp DNA sequence was cloned and analyzed. Screening of the database did not reveal any homologies with the known plant DNA sequences. Using the primers specific to the flanking regions of the marker sequence, PCR analysis of the polyploid wheat species and the diploid species of the section Sitopsis was carried out. In addition, using the cloned sequence as a molecular hybridization probe, RFLP analysis of the genomic DNA of these species was performed.
Shcherban'
A B
AB
Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, 630090 Russia.
Khlestkina
E K
EK
Salina
E A
EA
D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics*; D005819:Genetic Markers; D018745:Genome, Plant; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D014908:Triticum / Q000235:genetics*
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers