Adsorption and desorption behavior of plasmid DNA on ion-exchange membranes. Effect of salt valence and compaction agents.
In this study, we report the effect of salt type and compaction agents on adsorption and desorption behavior of plasmid DNA on strong anion-exchange membranes. Both divalent cations and compaction agents are known to reduce the effective charge density of plasmid DNA in solution, and compaction agents decrease the radius of gyration of plasmids. Differences in the batch uptake adsorption of a 6.1 kilo base pair plasmid in solution with sodium and magnesium salts were observed at low ionic strengths. Recoveries at high salt conditions, however, were independent of the cation, and measured only 63-76%. Similarly, no improvement in recoveries were observed when using sulfate rather than chloride anions as displacers. The compaction agents, spermine and spermidine, showed no strong effect on the uptake adsorption, capacity, or recovery of three different-sized plasmids on membrane sheets. It is recommended that further efforts to improve plasmid recoveries from anion-exchange membranes focus on properties of the adsorbent surface.
Teeters
Mark A
MA
Department of Chemical Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706-1619, USA.
Root
Thatcher W
TW
Lightfoot
Edwin N
EN
D000327:Adsorption; D000838:Anions; D002412:Cations; D004247:DNA / Q000737:chemistry*; D007475:Ion Exchange Resins; D008567:Membranes, Artificial; D010957:Plasmids / Q000737:chemistry*; D012492:Salts / Q000737:chemistry*
D000838:Anions; D002412:Cations; D007475:Ion Exchange Resins; D008567:Membranes, Artificial; D012492:Salts; D004247:DNA