No existence of translocus balancer to coordinate the expression and regulation of human hemoglobin genes in transgenic mice study.
All mammals use hemoglobin (Hb) to transport oxygen. Each Hb molecule is a tetramer of two pairs of unlike globin polypeptide chains. Equal amount of subunit globin chains derived from the corresponding alpha- and beta-like genes can always result during development though the two separate gene clusters are located on two different chromosomes and spatially transcribed within different nuclear domains. Disturbance of this balance will result in degradation or precipitation of the excessive globin chains, which is the character of various thalassemic syndromes. In previous studies, we had established two kinds of bacterial artificial chromosome (BAC) mediated transgenic mouse models, which contain respectively the entire human alpha- and beta-globin cluster. Here, we investigated the regulatory relationship between the two clusters by interbreeding these two kinds of transgenic mice. The levels of human alpha- and beta-mRNA in the various hybrid lines reflect the levels in the original transgenic lines that contain either the alpha- or beta-globin cluster alone. The results suggested that there is no apparent cross talk or regulatory interaction between the two human globin clusters in transgenic mice.
Huang
Yue
Y
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
Liu
De-Pei
DP
Feng
Dong-Xiao
DX
Wu
Min
M
Shen
Wei
W
Tang
Yi
Y
Tang
Xiao-Bin
XB
Liang
Chih-Chuan
CC
D000818:Animals; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics / Q000378:metabolism; D006454:Hemoglobins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D005810:Multigene Family / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis
D006454:Hemoglobins; D012333:RNA, Messenger; D005914:Globins