Genome-wide analysis of lipoprotein expression in Escherichia coli MG1655.
To gain insight into the cell envelope of Escherichia coli grown under aerobic and anaerobic conditions, lipoproteins were examined by using functional genomics. The mRNA expression levels of each of these genes under three growth conditions--aerobic, anaerobic, and anaerobic with nitrate--were examined by using both Affymetrix GeneChip E. coli antisense genome arrays and real-time PCR (RT-PCR). Many genes showed significant changes in expression level. The RT-PCR results were in very good agreement with the microarray data. The results of this study represent the first insights into the possible roles of unknown lipoprotein genes and broaden our understanding of the composition of the cell envelope under different environmental conditions. Additionally, these data serve as a test set for the refinement of high-throughput bioinformatic and global gene expression methods.
Brokx
Stephen J
SJ
Project CyberCell, Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada.
Ellison
Michael
M
Locke
Troy
T
Bottorff
Drell
D
Frost
Laura
L
Weiner
Joel H
JH
D004926:Escherichia coli / Q000235:genetics*; D016680:Genome, Bacterial; D008074:Lipoproteins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D008074:Lipoproteins