Neuronal traits are required for glucose-induced insulin secretion.
The transcriptional repressor RE1 silencer transcription factor (REST) is an important factor that restricts some neuronal traits to neurons. Since these traits are also present in pancreatic beta-cells, we evaluated their role by generating a model of insulin-secreting cells that express REST. The presence of REST led to a decrease in expression of its known target genes, whereas insulin expression and its cellular content were conserved. As a consequence of REST expression, the capacity to secrete insulin in response to mitochondrial fuels, a particularity of mature beta-cells, was impaired. These data provide evidence that REST target genes are required for an appropriate glucose-induced insulin secretion.
Abderrahmani
Amar
A
Department of Internal Medicine, University of Lausanne, CHUV-1011 Lausanne, Switzerland.
Niederhauser
Guy
G
Plaisance
Valérie
V
Haefliger
Jacques-Antoine
JA
Regazzi
Romano
R
Waeber
Gérard
G
D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D056970:Co-Repressor Proteins; D004396:Coloring Agents / Q000494:pharmacology; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism*; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013997:Time Factors; D014158:Transcription, Genetic; D014162:Transfection
D056970:Co-Repressor Proteins; D004396:Coloring Agents; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D007328:Insulin; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C485650:Rcor2 protein, mouse; D012097:Repressor Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; D012313:RNA; D011189:Potassium Chloride; C022616:thiazolyl blue; D005947:Glucose