Cytogenetic abnormalities and the failure of development after round spermatid injections.
OBJECTIVE
To assess cytologic and cytogenetic abnormalities following round spermatid injection.


METHODS
Prospective analysis.


METHODS
In vitro fertilization centers.


METHODS
Fourteen couples accepted to a round spermatid injection (ROSI) and preimplantation genetic diagnosis (PGD) program after appropriate counseling.


METHODS
ROSI, PGD, with fluorescence in situ hybridization for chromosome enumeration.


METHODS
Cytologic and cytogenetic abnormalities in oocytes, zygotes, and blastomeres.


RESULTS
The fertilization rate following ROSI was 36%. Only 11 of 143 (7.7%) oocytes developed to have several blastomeres. Cytologic and cytogenetic abnormalities accounted for the vast majority of blockage at oocyte, zygote, and early mitotic division stages. Four biopsied embryos were normal. These and seven others were implanted, but no pregnancy was achieved.


CONCLUSIONS
A PGD diagnosis for common aneuploidies and blastocyst stage transfer is feasible for ROSI cases. Failure with ROSI is cause primarily by chromosome abnormalities, so use of ROSI in assisted reproductive technologies should be limited.
Benkhalifa
Moncef
M
ART and Reproductive Genetics Unit, Istanbul Memorial Hospital, Istanbul, Turkey. atl78@aol.com
Kahraman
Semra
S
Biricik
Annil
A
Serteyl
Semra
S
Domez
Ersan
E
Kumtepe
Yacoup
Y
Qumsiyeh
Mazin B
MB
D002869:Chromosome Aberrations; D005314:Embryonic and Fetal Development; D005260:Female; D005306:Fertilization; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007267:Injections; D008297:Male; D011446:Prospective Studies; D013087:Spermatids / Q000648:ultrastructure*