A role for the Cdc14-family phosphatase Flp1p at the end of the cell cycle in controlling the rapid degradation of the mitotic inducer Cdc25p in fission yeast.
The Schizosaccaromyces pombe protein Flp1p belongs to a conserved family of serine-threonine-phosphatases. The founding member of this family, Saccharomyces cerevisiae Cdc14p, is required for inactivation of mitotic CDKs and reversal of CDK mediated phosphorylation at the end of mitosis, thereby bringing about the M-G1 transition. Initial studies of Flp1p suggest that it may play a different role to Cdc14p. Here we show that Flp1p is required for rapid degradation of the mitotic inducer Cdc25p at the end of mitosis, and that Cdc25p is a substrate of Flp1p in vitro. Down-regulation of Cdc25p activity by Flp1p may ensure a prompt inactivation of mitotic CDK complexes to trigger cell division. Our results suggest a regulatory mechanism, and a universal role, for Cdc14p like proteins in coordination of cytokinesis with other cell cycle events.
Esteban
Verónica
V
Departamento de Microbiología y Genética, Campus Miguel de Unamuno, Universidad de Salamanca/CSIC, 37007 Salamanca, Spain.
Blanco
Miguel
M
Cueille
Nathalie
N
Simanis
Viesturs
V
Moreno
Sergio
S
Bueno
Avelino
A
D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D048749:Cytokinesis; D015536:Down-Regulation; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005982:Glutathione Transferase / Q000378:metabolism; D008938:Mitosis; D009154:Mutation; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism*; D017027:Protein Tyrosine Phosphatases; D011994:Recombinant Proteins / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D020687:cdc25 Phosphatases / Q000235:genetics / Q000378:metabolism*
D018797:Cell Cycle Proteins; D011994:Recombinant Proteins; D029702:Schizosaccharomyces pombe Proteins; D005982:Glutathione Transferase; D010749:Phosphoprotein Phosphatases; C439269:Clp1 protein, S. pombe; D017027:Protein Tyrosine Phosphatases; D020687:cdc25 Phosphatases