title: Recombinant single-chain canine interleukin 12 induces interferon gamma mRNA expression in peripheral blood mononuclear cells of dogs with visceral leishmaniasis.
abstract: Canine visceral leishmaniasis poses important concerns for public health and veterinary medicine in many areas of the world. Resistance to it seems to be associated with cellular specific immune responses of the so-called Th1 type. Interleukin-12 (IL-12) is one of the most potent inducers of Th1 type of immune responses to co-administered antigens. Herein, the cloning of canine IL-12, as a single-chain fusion protein (sccaIL-12), and its expression in biologically active form in COS-7 cells is reported. Supernatants from these cells stimulated the expression of comparable amounts of interferon gamma mRNA in peripheral blood mononuclear cells from dogs with natural visceral leishmaniasis. In addition, after stimulation with sccaIL-12, there was no difference between interferon gamma mRNA expressions in peripheral blood mononuclear cells of dogs with visceral leishmaniasis and from normal healthy control animals.
authors.affiliation: Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Rua Waldemar Falcão, No. 121, Brotas, Salvador-Bahia, CEP 40295-001, Brazil.
authors.forename: Lenita Ramires
authors.initials: LR
authors.lastname: dos Santos
authors.forename: Stella Maria
authors.initials: SM
authors.lastname: Barrouin-Melo
authors.forename: Yung-Fu
authors.initials: YF
authors.lastname: Chang
authors.forename: John
authors.initials: J
authors.lastname: Olsen
authors.forename: Sean P
authors.initials: SP
authors.lastname: McDonough
authors.forename: Fred
authors.initials: F
authors.lastname: Quimby
authors.forename: Washington Luis Conrado
authors.initials: WL
authors.lastname: dos Santos
authors.forename: Lain Carlos
authors.initials: LC
authors.lastname: Pontes-de-Carvalho
authors.forename: Geraldo Gileno de Sá
authors.initials: GG
authors.lastname: Oliveira
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004283:Dog Diseases / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology; D004285:Dogs; D015870:Gene Expression; D066298:In Vitro Techniques; D007369:Interferon Inducers / Q000737:chemistry / Q000494:pharmacology*; D007371:Interferon-gamma / Q000235:genetics*; D018664:Interleukin-12 / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D007898:Leishmaniasis, Visceral / Q000188:drug therapy / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology
chemical_list: D007369:Interferon Inducers; D012333:RNA, Messenger; D011994:Recombinant Proteins; D018664:Interleukin-12; D007371:Interferon-gamma