title: [Effect of intraoperative radiation therapy on enzyme histochemistry of expression muscle of guinea pigs after injury of facial nerve].
abstract: OBJECTIVE
  To explore the mechanism of facial nerve recovery after radiotherapy by simulating surgical treatment of parotid gland carcinoma with reserving facial nerve and studying the ability of aerobic metabolism, transmission of neurotransmitter, variation of ultrastructure of motor end and mitochondrion after radiotherapy during operation.
  
  
  METHODS
  Animal models of treatment group(15Gy) and lethal dose group(17Gy) were established. Succinic dehydrogenase(SDH) and acetylcholine lipase(AchE) of orbicularis oris were measured after radiotherapy. Student's t test was used for statistical analysis.
  
  
  RESULTS
  The activity of SDH and AchE declined after radiotherapy. There was significant difference between treatment group and lethal dose group. One month later, there was a significant improvement in the activity of SDH and AchE in treatment group, while that of lethal dose group continued to decline.
  
  
  CONCLUSIONS
  After radiotherapy during parotid gland surgery, the activity of SDH and AchE, transmission of neurotransmitter, and the ability of aerobic metabolism decreased. The ultrastructure of motor end and mitochondrion was destroyed. The variation returned to preoperative levels in treatment group, while did not in lethal dose group.
authors.affiliation: Department of Oral and Maxillofacial Surgery,The First Affiliated Hospital,China Medical University. Shenyang 110001, Liaoning province, China. hansiyuan2002@yahoo.com.cn
authors.forename: Si-yuan
authors.initials: SY
authors.lastname: Han
authors.forename: Yu-xin
authors.initials: YX
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Song
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D005149:Facial Expression; D005152:Facial Muscles / Q000201:enzymology*; D005154:Facial Nerve / Q000473:pathology / Q000528:radiation effects*; D006168:Guinea Pigs; D006651:Histocytochemistry; D010307:Parotid Neoplasms / Q000532:radiotherapy / Q000601:surgery; D011878:Radiotherapy / Q000009:adverse effects*; D013385:Succinate Dehydrogenase / Q000378:metabolism*
chemical_list: D013385:Succinate Dehydrogenase; D000110:Acetylcholinesterase