Analysis of the S810L point mutation of the mineralocorticoid receptor in patients with pregnancy-induced hypertension.
OBJECTIVE
A missense mutation at codon 810 (Ser --> Leu) of the mineralocorticoid receptor was recently observed in a family with early manifestation of hypertension. Our objective was to determine if this mineralocorticoid receptor alterations is prevalent in patients with pregnancy-induced hypertension.


METHODS
Thirty-eight women with hypertension during pregnancy were tested for the mineralocorticoid receptor gene mutation. DNA was extracted out of blood leucocytes. PCR and automated DNA sequencing were used to analyze exon 6 for the S810L missense mutation. Anamnestical data concerning cardiovascular risk factors and family history were evaluated with a questionnaire. Pregnancy course and outcome were documented in all cases.


RESULTS
In 33 patients with pregnancy-induced hypertension and in five patients with exacerbation of preexisting hypertension in pregnancy no point mutations were found at codon 810 in exon 6.


CONCLUSIONS
Our data suggest that the S810L missense mutation of the mineralocorticoid receptor does not play a major role in the etiology of pregnancy-induced hypertension in a German /Turkish population.
Schmider-Ross
Annette
A
Department of Obstetrics and Gynecology, Charité, Campus Virchow-Klinikum, Humboldt-University, Berlin, Germany. annette.schmider@charite.de
Wirsing
M
M
Büscher
U
U
Neitzel
H
H
Krause
M
M
Henrich
W
W
Reles
A
A
Dudenhausen
J W
JW
D003062:Codon / Q000235:genetics; D005091:Exons / Q000235:genetics; D005260:Female; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D008429:Maternal Welfare; D020125:Mutation, Missense / Q000235:genetics; D017354:Point Mutation / Q000235:genetics; D011247:Pregnancy; D011249:Pregnancy Complications, Cardiovascular / Q000209:etiology*; D011338:Probability Theory; D018161:Receptors, Mineralocorticoid / Q000235:genetics*
D003062:Codon; D018161:Receptors, Mineralocorticoid