[Effect of repeated acute hypoxic treatment on the expression of alpha-synuclein in the mouse brain cortex].
An anti-alpha-synuclein (alpha-SYN) monoclonal antibody produced in our laboratory was used to investigate the effect of repeated acute hypoxic treatments on the expression of alpha-SYN in the mouse cerebral cortex. Western blot analysis showed that the expression levels of alpha-SYN in the cortex changed accordingly upon hypoxic exposure times, as that the alpha-synuclein level significantly increased after the first hypoxic exposure and then dropped down to the background level after the fourth hypoxic exposure. Immunohistochemical staining revealed that the alpha-SYN-immunopositive substance was localized not only in the nerve endings, but also within the nuclei of some neurons. The cell density of the neurons with alpha-SYN immunopositive nuclei was increased significantly after the first hypoxic exposure but returned back to control levels after the fourth hypoxic exposure. Our results indicate that both of the alpha-SYN expression level in the brain and the number of the neurons with alpha-SYN positive nuclei are affected by the repeated acute hypoxic treatments and that this modification is hypoxic time-dependent. The mechanism and the physiological significance underlying these changes need to be further investigated.
Yu
Shun
S
Department of Neurobiology, Beijing Institute of Geriatrics, Xuan Wu Hospital, Capital University of Medical Sciences.
Liu
Xue-Mei
XM
Li
Yao-Hua
YH
Lu
Guo-Wei
GW
Chen
Biao
B
D000818:Animals; D001921:Brain / Q000098:blood supply*; D002545:Brain Ischemia / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D019194:Ischemic Preconditioning; D051379:Mice; D008807:Mice, Inbred BALB C; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009474:Neurons / Q000378:metabolism; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051843:Synucleins; D051844:alpha-Synuclein
D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; C497605:Snca protein, mouse; D051843:Synucleins; D051844:alpha-Synuclein