Determination of lower sub ppt levels of environmental analytes using high-powered concentration system and high-performance liquid chromatography with fluorescence detection.
The proposed method was successful in the determination and separation of lower sub ppt levels of polycyclic aromatic hydrocarbons (PAHs). In this study, a new phase separation phenomenon (i.e., homogeneous liquid-liquid extraction) was developed that rapidly and simply separated an immiscible liquid phase from water-miscible organic solvents. One liter of sample solution was preconcentrated by a solid phase extraction method, then the obtained eluate (5 mL) was further preconcentrated to just 20 microL by this homogeneous liquid-liquid extraction. The 20 [micro sign]L sedimented phase was directly injected into the high-performance liquid chromatography with fluorescence detection (FL-HPLC). The entire preconcentration factor was 50,000-fold. Six kinds of PAHs were determined in the range of 3.0 x 10(-18) approximately 4.5 x 10(-11) mol L(-1). These chemicals were also satisfactorily separated.
Akiyama
Ryotarou
R
Department of Materials Science, Faculty of Engineering, Ibaraki University, Nakanarusawa 4-12-1, Hitachi, Ibaraki 316-8511, Japan.
Takagai
Yoshitaka
Y
Igarashi
Shukuro
S
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D011891:Rain; D014874:Water Pollutants, Chemical / Q000032:analysis*
D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical