In vivo antiosteoporotic activity of a fraction of Dioscorea spongiosa and its constituent, 22-O-methylprotodioscin.
The antiosteoporotic activity of the 90 % EtOH fraction of the water extract of rhizomes of Dioscorea spongiosa and methylprotodioscin, its major constituent, were examined in the model of postmenopausal bone loss using ovariectomized (OVX) rats or mice. After 6 weeks treatment, the proximal tibia of rats or mice and the distal femora of mice were scanned by peripheral quantitative computed tomography (pQCT). Both the 90 % EtOH fraction (100 mg/kg/d) and methylprotodioscin (50 mg/kg/d) significantly inhibited bone loss in bone mineral content (BMC) and bone mineral density (BMD) in total, cancellous and cortical bones, and the decrease in bone strength indexes induced by OVX, without side effect on the uterus.
Yin
Jun
J
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Japan.
Tezuka
Yasuhiro
Y
Kouda
Kyoji
K
Le Tran
Quan
Q
Miyahara
Tatsuro
T
Chen
Yingjie
Y
Kadota
Shigetoshi
S
D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D027742:Dioscorea; D004144:Diosgenin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology* / Q000627:therapeutic use; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D010024:Osteoporosis / Q000517:prevention & control*; D010052:Ovariectomy; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
D010936:Plant Extracts; D012503:Saponins; C425324:methyl protodioscin; D004144:Diosgenin