Three-dimensional cellular-level imaging using full-field optical coherence tomography.
An ultrahigh-resolution full-field optical coherence tomography (OCT) system has been developed for cellular-level imaging of biological media. The system is based on a Linnik interference microscope illuminated with a tungsten halogen lamp, associated with a high-resolution CCD camera. En face tomographic images are produced in real time, with the best spatial resolution ever achieved in OCT (0.7 microm x 0.9 microm, axial x transverse). A shot-noise limited detection sensitivity of 80 dB can be reached with an acquisition time per image of 1 s. Images of animal ophthalmic biopsies and vegetal tissues are shown.
Dubois
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Laboratoire d'Optique Physique, Ecole Supérieure de Physique et Chimie Industrielles, Centre National de la Recherche Scientifique, UPR A0005, 10 rue Vauquelin, F-75231 Paris Cedex 5, France.
Moneron
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Grieve
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Boccara
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AC
D000818:Animals; D005123:Eye / Q000166:cytology*; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008857:Microscopy, Interference / Q000379:methods*; D018515:Plant Leaves / Q000166:cytology*; D051381:Rats; D014026:Nicotiana / Q000166:cytology; D041623:Tomography, Optical Coherence / Q000379:methods*