[Cloning of mouse p27Kip1 and investigation on its role in apoptosis and survival of peripheral blood B lymphocytes].
OBJECTIVE
To clone the mouse p27(Kip1) gene and analyze its expression and possible function in the signaling through antigen receptor and CD40 in peripheral blood B cells.


METHODS
Mouse p27(Kip1)was cloned from cultured B cells by RT-PCR. Apoptosis of the B cells was detected by flow cytometry. Expression of p27(Kip1)gene was detected by Northern blot.


RESULTS
Mouse p27(Kip1)gene was successfully cloned. In consistence with B cell apoptosis or survival, cross-linking of antigen receptor also induced up-regulation of p27(Kip1)in mouse B cells, which was blocked by CD40 signaling. Antigen receptor-mediated B cell apoptosis was blocked by antisense oligonucleotides directed against p27(Kip1).


CONCLUSIONS
These data suggest that p27(Kip1)may be involved in apoptosis and survival of B cells mediated by antigen receptor and CD40 signals, respectively.
Wang
Li-li
LL
Laboratory of Clinical Pharmacology of Chinese Materiae Medica, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Han
Hua
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D017209:Apoptosis; D001402:B-Lymphocytes / Q000166:cytology* / Q000378:metabolism; D019013:CD40 Antigens / Q000276:immunology*; D002470:Cell Survival; D002478:Cells, Cultured; D003001:Cloning, Molecular; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D000911:Antibodies, Monoclonal; D019013:CD40 Antigens; C493961:Cdkn1b protein, mouse; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D050760:Cyclin-Dependent Kinase Inhibitor p27