Development of a hemi-nested RT-PCR method for the specific determination of European Bat Lyssavirus 1. Comparison with other rabies diagnostic methods.
A simplified hemi-nested reverse transcriptase polymerase chain reaction (hnRT-PCR) has been developed to determine specifically the European Bat Lyssavirus 1 (EBLV-1) nucleoprotein gene. The specificity of this method was determined by using the seven genotypes of lyssavirus by RT-PCR, Southern blot and sequence analysis. Compared to the rabies diagnostic methods, the hnRT-PCR showed a higher sensitivity for the detection of small amounts of EBLV-1 virus. In view of these results, we suggest this new hnRT-PCR should be performed for the epidemiological survey of bat colonies, also providing rapid detection and genotyping of EBLV-1 until now encountered in all naturally infected bats in France.
Picard-Meyer
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Agence Française de Sécurité Sanitaire des Aliments, AFSSA-Nancy, Domaine de Pixérécourt, BP 9, Malzéville F-54220, France. e.picard@nancy.afssa.fr
Bruyère
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Barrat
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Tissot
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Barrat
M J
MJ
Cliquet
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D000595:Amino Acid Sequence; D000818:Animals; D015139:Blotting, Southern; D001921:Brain / Q000821:virology; D002685:Chiroptera / Q000821:virology*; D046508:Culture Techniques; D017931:DNA Primers; D005455:Fluorescent Antibody Technique; D018114:Lyssavirus / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000235:genetics; D011818:Rabies / Q000175:diagnosis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D018353:Rhabdoviridae Infections / Q000175:diagnosis* / Q000453:epidemiology
D017931:DNA Primers; D009698:Nucleoproteins