title: Inhibitory effect of pyrimidine and purine nucleotides on the multiplication of Babesia gibsoni: possible cause of low parasitemia and simultaneous reticulocytosis in canine babesiosis.
abstract: The present study was conducted to determine the cause of low parasitemia and simultaneous reticulocytosis in canine babesiosis. The parasitemia was significantly decreased in in vitro cultures of Babesia gibsoni by the pretreatment of host canine erythrocytes with lead acetate, which is a specific inhibitor of pyrimidine 5'-nucleotidase subclass I (P5N-I). The serum from dogs chronically infected with B. gibsoni did not decrease the activities of hexokinase, glucose-6-phosphate dehydrogenase or 6-phosphogluconate dehydrogenase in canine reticulocytes, although it was previously reported that this serum had inhibitory effects on both the maturation of reticulocytes and the canine P5N-I and purine-specific 5'-nucleotidase activities. Furthermore, the in vitro multiplication of B. gibsoni was significantly inhibited by pyrimidine nucleotides such as cytidine 5'-monophosphate (5'-CMP), which is preferentially catalyzed by P5N-I and also inhibits the morphological maturation of canine reticulocytes. Purine nucleotides such as inosine 5'-monophosphate (5'-IMP) also had an inhibitory effect on the multiplication of this parasite. These results suggest that nucleotides such as 5'-CMP and 5'-IMP might accumulate in young erythrocytes and/or serum in dogs infected with B. gibsoni as a result of the decreased activity of erythrocyte 5'-nucleotidase, and the accumulation of these nucleotides might inhibit the multiplication of this parasite and simultaneously retard the maturation of reticulocytes. The results obtained from the in vitro examinations in the present study may partially clarify the relationship between low parasitemia and simultaneous reticulocytosis in vivo in canine babesiosis.
authors.affiliation: Laboratory of Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Mohammad Alamgir
authors.initials: MA
authors.lastname: Hossain
authors.forename: Osamu
authors.initials: O
authors.lastname: Yamato
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Maede
mesh_terms: D015720:5'-Nucleotidase / Q000037:antagonists & inhibitors; D000818:Animals; D001403:Babesia / Q000187:drug effects* / Q000254:growth & development; D001404:Babesiosis / Q000378:metabolism / Q000662:veterinary*; D003568:Cytidine Monophosphate / Q000378:metabolism / Q000494:pharmacology*; D004283:Dog Diseases / Q000378:metabolism / Q000469:parasitology*; D004285:Dogs; D004912:Erythrocytes / Q000378:metabolism / Q000469:parasitology; D007291:Inosine Monophosphate / Q000378:metabolism / Q000494:pharmacology*; D009942:Organometallic Compounds / Q000378:metabolism; D045262:Reticulocytosis / Q000187:drug effects*
chemical_list: D009942:Organometallic Compounds; D007291:Inosine Monophosphate; D015720:5'-Nucleotidase; D003568:Cytidine Monophosphate; C008261:lead acetate