Degradation pathways of cyclic alkanes in Rhodococcus sp. NDKK48.
The degradation pathways for cyclic alkanes (c-alkanes) in Rhodococcus sp. NDKK48 were investigated. Strain NDKK48 used dodecylcyclohexane as a sole carbon and energy source, and five metabolites in the dodecylcyclohexane degradation pathway were detected by gas-chromatography/mass spectra. The metabolites were identified as cyclohexanecarboxylic acid, cyclohexylacetic acid, 1-cyclohexene-1-acetic acid, 4-dodecylcyclohexanol, and 4-dodecylcyclohexanone. The strain degrades dodecylcyclohexane via a ring oxidation pathway and an alkyl side chain oxidation pathway. Cyclohexanecarboxylic acid was further oxidized to muconic acid via 1-cyclohexene-1-carboxylic acid and benzoic acid, and the muconic acid was finally used by strain NDKK48 for growth. Methylcyclohexane and cyclohexane were co-oxidized with hexadecane by strain NDKK48. Methylcyclohexane was degraded via a ring oxidation pathway, and the degradation pathway contained part of the Baeyer-Villiger oxidation for ring cleavage. Cyclohexane was also degraded by the same pathway as methylcyclohexane. Thus, strain NDKK48 has two pathways for the complete degradation of c-alkanes.
Koma
D
D
Department of Bioscience and Technology, Faculty of Science and Engineering, Ritsumeikan University, Kusatsu, 525-8577 Shiga, Japan.
Sakashita
Y
Y
Kubota
K
K
Fujii
Y
Y
Hasumi
F
F
Chung
S Y
SY
Kubo
M
M
D001673:Biodegradation, Environmental; D003510:Cyclohexanes / Q000737:chemistry / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D015394:Molecular Structure; D012240:Rhodococcus / Q000378:metabolism*; D013997:Time Factors
D003510:Cyclohexanes