title: Michael addition of imidazole with acrylates catalyzed by alkaline protease from Bacillus subtilis in organic media.
abstract: A new activity of alkaline protease from Bacillus subtilis for Michael addition reactions of imidazole, 4-nitro-1H-imidazole and 2-methyl-4-nitro-1H-imidazole with acrylates and acrylic acid was investigated. The reactions were carried out in pyridine at 50 degrees C for 72 h. Five N-substituted imidazole derivatives were obtained using acrylate esters, but not acrylic acid, in yields from 62% to 76%.
authors.affiliation: Department of Chemistry, Zhejiang University, Hangzhou 310027, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Cai
authors.forename: Shi-Ping
authors.initials: SP
authors.lastname: Yao
authors.forename: Qi
authors.initials: Q
authors.lastname: Wu
authors.forename: Xian-Fu
authors.initials: XF
authors.lastname: Lin
mesh_terms: D000179:Acrylates / Q000737:chemistry*; D001412:Bacillus subtilis / Q000737:chemistry / Q000201:enzymology*; D003546:Cysteine Endopeptidases / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry*; D012997:Solvents
chemical_list: D000179:Acrylates; D007093:Imidazoles; D011725:Pyridines; D012997:Solvents; C029899:imidazole; D003546:Cysteine Endopeptidases; C065940:alkaline peptidase; C036658:acrylic acid; C023666:pyridine