Effect of cyclin G2 on proliferative ability of SGC-7901 cell.
OBJECTIVE
To study the effect of cyclin G2 on proliferation of gastric adenocarcinoma cell line-SGC-7901 cell in vitro.


METHODS
By use of cation lipofectamine transfection reagent, the pIRES-G2 and pIRESneo plasmids were transferred into SGC-7901cell line. Anticlones were selected by G418. Positive clones were observed and counted using Giemsa staining. Cell proliferative ability was assayed by MTT.


RESULTS
(1) The clone number of pIRES-G2 group decreased, clone volume reduced. The number of cell clones in pIRESneo group was 87+/-3, that of pIRES-G2 group was 53+/-4, occupying 60.1% of pIRESneo group, there was significant difference obviously (P<0.01, t=15.45). (2) The average absorbance of clone cell obtained by stable transfection of pIRES-G2 at 570 nm was 1.6966+/-0.2125, the average absorbance of clone cell obtained by stable transfection of pIRESneo at 570 nm was 2.1182+/-0.3675, there was significant difference between them (P<0.01, t=3.412).


CONCLUSIONS
Cyclin G2 can inhibit SGC-7901cell proliferative ability obviously, it may be a negative regulator in cell cycle regulation.
Liu
Jie
J
Experimental Technology Center, China Medical University, Shenyang 110001, Liaoning Province, China.
Cui
Ze-Shi
ZS
Luo
Yang
Y
Jiang
Li
L
Man
Xiao-Hui
XH
Zhang
Xue
X
D000230:Adenocarcinoma / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology; D002455:Cell Division / Q000502:physiology*; D045744:Cell Line, Tumor; D056767:Cyclin G2; D016213:Cyclins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D013274:Stomach Neoplasms / Q000378:metabolism; D014162:Transfection
C101593:CCNG2 protein, human; D056767:Cyclin G2; D016213:Cyclins