title: Rhamnogalacturonan lyase reveals a unique three-domain modular structure for polysaccharide lyase family 4.
abstract: Rhamnogalacturonan lyase (RG-lyase) specifically recognizes and cleaves alpha-1,4 glycosidic bonds between L-rhamnose and D-galacturonic acids in the backbone of rhamnogalacturonan-I, a major component of the plant cell wall polysaccharide, pectin. The three-dimensional structure of RG-lyase from Aspergillus aculeatus has been determined to 1.5 A resolution representing the first known structure from polysaccharide lyase family 4 and of an enzyme with this catalytic specificity. The 508-amino acid polypeptide displays a unique arrangement of three distinct modular domains. Each domain shows structural homology to non-catalytic domains from other carbohydrate active enzymes.
authors.affiliation: Centre for Crystallographic Studies, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen Ø, DK, Denmark. michael.mcdonough@chemistry.oxford.ac.uk
authors.forename: Michael A
authors.initials: MA
authors.lastname: McDonough
authors.forename: Renuka
authors.initials: R
authors.lastname: Kadirvelraj
authors.forename: Pernille
authors.initials: P
authors.lastname: Harris
authors.forename: Jens-Christian N
authors.initials: JC
authors.lastname: Poulsen
authors.forename: Sine
authors.initials: S
authors.lastname: Larsen
mesh_terms: D000595:Amino Acid Sequence; D001230:Aspergillus / Q000201:enzymology; D002384:Catalysis; D020134:Catalytic Domain; D002473:Cell Wall / Q000378:metabolism; D018360:Crystallography, X-Ray; D006603:Hexuronic Acids / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010368:Pectins / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D011133:Polysaccharide-Lyases / Q000737:chemistry* / Q000502:physiology; D011485:Protein Binding; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012210:Rhamnose / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
chemical_list: D006603:Hexuronic Acids; D010455:Peptides; C007819:galacturonic acid; D010368:Pectins; D011133:Polysaccharide-Lyases; C426005:rhamnogalacturonan lyase 11A; D012210:Rhamnose