IL-9 increases the expression of several cytokines in activated mast cells, while the IL-9-induced IL-9 production is inhibited in mast cells of histamine-free transgenic mice.
Histamine and IL-9 are suspected to play an important role in the pathogenesis of asthmatic and allergic reactions. Mast cells store a large amount of histamine in their granules and are capable of producing different cytokines upon stimulation. In this study we show that mast cells stimulated by IL-9 and ionomycin or IL-9 and antigen-specific IgE/antigen express several cytokines at mRNA level, among them are IL-5, IL-4, IL-10, IL-9, IL-13, IL-1beta, IL-1Ra, IL-6 and MIF. Furthermore, both IL-9 and ionomycin are needed for the production of these cytokines in great quantities, which is mediated through the production of IL-1beta. Histamine-free mast cells respond by a markedly decreased IL-9 expression to this stimulation. Our results show that this IL-9-induced IL-9 production may result in a positive feedback loop in mast cells and the lack of histamine disturbs this loop, which may serve as an explanation for the reduced asthmatic symptoms, observed in histamine-free mice.
Wiener
Zoltan
Z
Department of Genetics, Cell and Immunobiology, Semmelweis University of Medicine, Nagyvárad tér 4, Budapest 1089, Hungary.
Falus
Andras
A
Toth
Sara
S
D000818:Animals; D000941:Antigens / Q000276:immunology; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D005434:Flow Cytometry; D006632:Histamine / Q000378:metabolism; D007073:Immunoglobulin E / Q000276:immunology; D007375:Interleukin-1 / Q000378:metabolism; D016906:Interleukin-9 / Q000378:metabolism*; D015759:Ionomycin / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D007700:Kinetics; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D051379:Mice
D000941:Antigens; D016207:Cytokines; D007375:Interleukin-1; D016906:Interleukin-9; D007476:Ionophores; D007073:Immunoglobulin E; D015759:Ionomycin; D006632:Histamine