title: The portal protein plays essential roles at different steps of the SPP1 DNA packaging process.
abstract: A large number of viruses use a specialized portal for entry of DNA to the viral capsid and for its polarized exit at the beginning of infection. These families of viruses assemble an icosahedral procapsid containing a portal protein oligomer in one of its 12 vertices. The viral ATPase (terminase) interacts with the portal vertex to form a powerful molecular motor that translocates DNA to the procapsid interior against a steep concentration gradient. The portal protein is an essential component of this DNA packaging machine. Characterization of single amino acid substitutions in the portal protein gp6 of bacteriophage SPP1 that block DNA packaging identified sequential steps in the packaging mechanism that require its action. Gp6 is essential at early steps of DNA packaging and for DNA translocation to the capsid interior, it affects the efficiency of DNA packaging, it is a central component of the headful sensor that determines the size of the packaged DNA molecule, and is essential for closure of the portal pore by the head completion proteins to prevent exit of the DNA encapsidated. Functional regions of gp6 necessary at each step are identified within its primary structure. The similarity between the architecture of portal oligomers and between the DNA packaging strategies of viruses using portals strongly suggests that the portal protein plays the same roles in a large number of viruses.
authors.affiliation: Instituto de Tecnologia Química e Biológica, 2781-901 Oeiras, Portugal.
authors.forename: Anabela
authors.initials: A
authors.lastname: Isidro
authors.forename: Adriano O
authors.initials: AO
authors.lastname: Henriques
authors.forename: Paulo
authors.initials: P
authors.lastname: Tavares
mesh_terms: D019943:Amino Acid Substitution; D017103:Bacillus Phages / Q000502:physiology*; D001412:Bacillus subtilis / Q000821:virology*; D002213:Capsid / Q000378:metabolism*; D042003:DNA Packaging; D009154:Mutation; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D014764:Viral Proteins; C115880:portal protein, Bacteriophage SPP1