Transgenic Cre expression mice for generation of erythroid-specific gene alterations.
Transgenic mice that express Cre recombinase in erythroid cell lineages were developed so that genes affecting erythropoiesis/hematopoiesis may be altered without necessarily affecting fetus viability. A micro-LCR cassette-beta-globin promoter-Cre recombinase gene (microLCR-betapr-Cre) construct was synthesized and used to generate transgenic mice. Concurrently, we produced mice containing a microLCR-loxP-flanked beta sickle gene (microLCR-loxP-beta(S)-loxP) construct. microLCR-betapr-Cre mice with intact transgenes in variable copy number were identified. Cre expression was assessed by RNAse protection and RT-PCR. Cre function was ascertained by breeding to microLCR-loxP-beta(S)-loxP mice. We demonstrate that beta(S) expression was not detected in the blood of bigenics, but the gene was present in nonerythroid cells. Thus, excision of the loxP-flanked beta(S) gene was restricted to erythroid cell lineages.
Peterson
Kenneth R
KR
Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, Kansas 66160-7421, USA. kpeterson@kumc.edu
Fedosyuk
Halyna
H
Zelenchuk
Lesya
L
Nakamoto
Betty
B
Yannaki
Evangelia
E
Stamatoyannopoulos
George
G
Ciciotte
Steven
S
Peters
Luanne L
LL
Scott
Linda M
LM
Papayannopoulou
Thalia
T
D000818:Animals; D001483:Base Sequence; D002469:Cell Separation; D017931:DNA Primers; D004912:Erythrocytes / Q000378:metabolism*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D017930:Genes, Reporter; D051379:Mice; D008822:Mice, Transgenic; D045522:Recombinases / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D045522:Recombinases