title: Ring-shaped architecture of RecR: implications for its role in homologous recombinational DNA repair.
abstract: RecR, together with RecF and RecO, facilitates RecA loading in the RecF pathway of homologous recombinational DNA repair in procaryotes. The human Rad52 protein is a functional counterpart of RecFOR. We present here the crystal structure of RecR from Deinococcus radiodurans (DR RecR). A monomer of DR RecR has a two-domain structure: the N-terminal domain with a helix-hairpin-helix (HhH) motif and the C-terminal domain with a Cys4 zinc-finger motif, a Toprim domain and a Walker B motif. Four such monomers form a ring-shaped tetramer of 222 symmetry with a central hole of 30-35 angstroms diameter. In the crystal, two tetramers are concatenated, implying that the RecR tetramer is capable of opening and closing. We also show that DR RecR binds to both dsDNA and ssDNA, and that its HhH motif is essential for DNA binding.
authors.affiliation: Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul, Korea.
authors.forename: Byung Il
authors.initials: BI
authors.lastname: Lee
authors.forename: Kyoung Hoon
authors.initials: KH
authors.lastname: Kim
authors.forename: Soo Jeong
authors.initials: SJ
authors.lastname: Park
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Eom
authors.forename: Hyun Kyu
authors.initials: HK
authors.lastname: Song
authors.forename: Se Won
authors.initials: SW
authors.lastname: Suh
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D034301:Deinococcus / Q000737:chemistry*; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D051721:Rad52 DNA Repair and Recombination Protein; D011995:Recombination, Genetic; D016415:Sequence Alignment
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C497194:RAD52 protein, human; D051721:Rad52 DNA Repair and Recombination Protein; C065831:RecR protein, Bacteria; D004247:DNA