title: Actinobacillus pleuropneumoniae metalloprotease: cloning and in vivo expression.
abstract: The complete amino acid and nucleotide sequence of a secreted metalloprotease produced by Actinobacillus pleuropneumoniae serotype 1 is reported. A clone showing proteolytic activity in cell-free culture media was selected from a genomic library of A. pleuropneumoniae serotype 1 in pUC 19. The sequence obtained contained an open reading frame encoding a protein with 869 amino acids. This protein was identified as a zinc neutral-metalloprotease belonging to the aminopeptidase family, with a predicted molecular weight of approximately 101 kDa. This sequence showed high homology with other predicted or sequenced aminopeptidases reported for different Gram-negative bacteria. Expression of the protease was observed in lung tissue from pigs that died of porcine pleuropneumonia suggesting a role in pathogenesis.
authors.affiliation: Carrera de Biología, Facultad de Estudios Superiores Iztacala, UNAM, Av. de los Barrios #1, Los Reyes Iztacala, Tlalnepantla, Estado de México 54090, Mexico.
authors.forename: Octavio
authors.initials: O
authors.lastname: García González
authors.forename: Rosa M
authors.initials: RM
authors.lastname: García
authors.forename: Mireya
authors.initials: M
authors.lastname: de la Garza
authors.forename: Sergio
authors.initials: S
authors.lastname: Vaca
authors.forename: Gloria Luz
authors.initials: GL
authors.lastname: Paniagua
authors.forename: Ricardo
authors.initials: R
authors.lastname: Mejía
authors.forename: Víctor R
authors.initials: VR
authors.lastname: Tenorio
authors.forename: Erasmo
authors.initials: E
authors.lastname: Negrete-Abascal
mesh_terms: D000189:Actinobacillus Infections / Q000382:microbiology / Q000662:veterinary; D016977:Actinobacillus pleuropneumoniae / Q000201:enzymology* / Q000235:genetics* / Q000472:pathogenicity; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005798:Genes, Bacterial; D008168:Lung / Q000473:pathology; D045726:Metalloproteases / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D011001:Pleuropneumonia / Q000382:microbiology / Q000662:veterinary; D011994:Recombinant Proteins / Q000378:metabolism; D017385:Sequence Homology; D013552:Swine / Q000382:microbiology; D013553:Swine Diseases / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D011994:Recombinant Proteins; D045726:Metalloproteases