Detection of multidrug resistant gene 1 in pancreatic cancer.
BACKGROUND
The results are conflicting in detecting P-glycoprotein (P-gp) in pancreatic cancer. The aim of this study was to detect the expression of multidrug resistant gene 1 (MDR1) in pancreatic cancer cell lines.


METHODS
MDR1 mRNA and P-gp were detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical assay (IHC) in three pancreatic cancer cell lines SW1990, CAPAN-1 and P3. P-gp functions were evaluated through the rhodamine extrusion test.


RESULTS
Two of the three cell lines expressed MDR1 positively at different levels. The rhodamine extrusion test showed that the percentage of positive cells in MDR(+) cells was significantly lower than that in MDR1(-) cells. The results of IHC, RT-PCR and the rhodamine extrusion test were consistent with each other.


CONCLUSIONS
All of these methods are reliable in the detection of MDR1 in pancreatic cancer tissue, thus providing a guide for clinical chemotherapy of pancreatic cancer.
Zhao
Yu-Pei
YP
Department of General Surgery, Peking Union Medical College Hospital, Beijing 100730, China. zhao8028@263.net
Zhang
Li-Yang
LY
Liao
Quan
Q
Guo
Jun-Chao
JC
Chen
Ge
G
Li
Jia-Yi
JY
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D015870:Gene Expression / Q000235:genetics; D019450:Genes, MDR / Q000235:genetics*; D006801:Humans; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D012333:RNA, Messenger