title: Influence of weaning and effect of post weaning dietary zinc and copper on electrophysiological response to glucose, theophylline and 5-HT in piglet small intestinal mucosa.
abstract: This study aimed to examine how weaning and how dietary zinc and/or copper fed post weaning may affect the electrophysiological response to glucose and to chloride secretagogues in piglet small intestine in vitro. Study 1 included 54 piglets (six litters of nine piglets). One piglet from every litter was killed 1 day before weaning. The remaining 48 piglets were allocated at weaning (28 d) to four dietary zinc treatments and subsequently killed 1-2, 5-6 or 14-15 days after weaning. Study 2 included 48 piglets (six litters of eight piglets) allocated to four dietary treatments, consisting of high or low dietary zinc with or without high dietary copper. All piglets in study 2 were killed 5-7 days after weaning. The in vitro studies in Ussing chambers showed that weaning resulted in increased ileal glucose absorption as well as increased neuroendocrine-regulated (activated by 5-HT) and cAMP-dependent (activated by theophylline) chloride secretion. High zinc supplementation reduced the responses to 5-HT and theophylline. The study did not reveal any influence of copper on these parameters. It is concluded that the positive effect of zinc supplementation on diarrhoea in weaned piglets may be due to zinc reducing the intestinal mucosal susceptibility to secretagogues that activate chloride secretion.
authors.affiliation: Department of Animal Nutrition and Physiology, Danish Institute of Agricultural Sciences, Research Centre Foulum, 8830 Tjele, Denmark. dorthe.carlson@agrsci.dk
authors.forename: Dorthe
authors.initials: D
authors.lastname: Carlson
authors.forename: Hanne Damgaard
authors.initials: HD
authors.lastname: Poulsen
authors.forename: Jakob
authors.initials: J
authors.lastname: Sehested
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002712:Chlorides / Q000378:metabolism; D003300:Copper / Q000008:administration & dosage / Q000494:pharmacology*; D003967:Diarrhea / Q000188:drug therapy / Q000209:etiology / Q000662:veterinary; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology; D007421:Intestine, Small / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology; D018408:Patch-Clamp Techniques; D012701:Serotonin / Q000494:pharmacology; D013552:Swine / Q000254:growth & development* / Q000502:physiology; D013806:Theophylline / Q000494:pharmacology; D014886:Weaning; D015032:Zinc / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D002712:Chlorides; D012701:Serotonin; D003300:Copper; D013806:Theophylline; D005947:Glucose; D015032:Zinc