Ubiquitin-like polypeptide inhibits cAMP-induced p38 MAPK activation in Th2 cells.
Ubi-L, an isoform of the monoclonal nonspecific suppressor factor (MNSF), is an 8.5-kDa ubiquitin-like polypeptide. Ubi-L exhibits an antigen-nonspecific immunosuppressive function on various target cells including murine T helper type 2 (Th2) clone, D10 cells. Ubi-L specifically binds to cell surface receptors on D10 cells. In this study, we observed that Ubi-L inhibited cAMP-induced IL-5 mRNA expression in D10 cells but not in thymoma cell line EL4. In addition, Ubi-L effectively inhibited cAMP-induced p38 MAPK activation in D10 cells. Ubi-L also showed inhibitory activity on IL-5 and IL-13 production by D10 cells stimulated with phorbol ester plus dibutyryl cAMP. Furthermore, Ubi-L inhibited IL-4 production in Th2 cells derived from primary CD4+ T cells.
Nakamura
Morihiko
M
Cooperative Medical Research Center, Shimane Medical University, School of Medicine, Izume, Japan. nkmr0515@med.shimane-u.ac.jp
Tanigawa
Yoshinori
Y
D000818:Animals; D015152:Blotting, Northern; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism*; D018793:Interleukin-13 / Q000378:metabolism; D015848:Interleukin-5 / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000378:metabolism; D018418:Th2 Cells / Q000201:enzymology* / Q000378:metabolism; D013997:Time Factors; D014452:Ubiquitins / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors*
D018793:Interleukin-13; D015848:Interleukin-5; D012333:RNA, Messenger; D014452:Ubiquitins; D000242:Cyclic AMP; D048051:p38 Mitogen-Activated Protein Kinases