title: Aluminum fluoride affects the structure and functions of cell membranes.
abstract: No useful biological function for aluminum has been found. To the contrary, it might play an important role in several pathologies, which could be related to its interactions with cell membranes. On the other hand, fluoride is a normal component of body fluids, soft tissues, bones and teeth. Its sodium salt is frequently added to drinking water to prevent dental caries. However, large doses cause severe pathological alterations. In view of the toxicity of Al(3+) and F(-) ions, it was thought of interest to explore the damaging effects that AlF(3) might induce in cell membranes. With this aim, it was incubated with human erythrocytes, which were examined by phase contrast and scanning electron microscopy, and molecular models of biomembranes. The latter consisted of large unilamellar vesicles (LUV) of dimyristoylphosphatidylcholine (DMPC) and bilayers of DMPC and dimyristoylphosphatidylethanolamine (DMPE) which were studied by fluorescence spectroscopy and X-ray diffraction, respectively. In order to understand the effects of AlF(3) on ion transport (principally sodium and chloride) we used the isolated toad skin to which electrophysiological measurements were applied. It was found that AlF(3) altered the shape of erythrocytes inducing the formation of echinocytes. This effect was explained by X-ray diffraction which revealed that AlF(3) perturbed the structure of DMPC, class of lipids located in the outer monolayer of the erythrocyte membrane. This result was confirmed by fluorescence spectroscopy on DMPC LUV. The biphasic (stimulatory followed by inhibitory) effects on the isolated skin suggested changes in apical Cl(-) secretion and moderate ATPase inactivation.
authors.affiliation: Faculty of Chemical Sciences, University of Concepcion, Casilla 160-C, Concepcion, Chile. msuwalsk@udec.cl
authors.forename: M
authors.initials: M
authors.lastname: Suwalsky
authors.forename: B
authors.initials: B
authors.lastname: Norris
authors.forename: F
authors.initials: F
authors.lastname: Villena
authors.forename: F
authors.initials: F
authors.lastname: Cuevas
authors.forename: P
authors.initials: P
authors.lastname: Sotomayor
authors.forename: P
authors.initials: P
authors.lastname: Zatta
mesh_terms: D000328:Adult; D017607:Aluminum Compounds / Q000633:toxicity*; D018929:Cell Culture Techniques; D002462:Cell Membrane / Q000187:drug effects* / Q000502:physiology; D004594:Electrophysiology; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology; D005459:Fluorides / Q000633:toxicity*; D006801:Humans; D008297:Male; D008958:Models, Molecular; D013050:Spectrometry, Fluorescence; D014961:X-Ray Diffraction
chemical_list: D017607:Aluminum Compounds; D005459:Fluorides; C032311:aluminum fluoride