Effect of gamma-hydroxybutyric acid on lipid peroxidation and tissue lactate level in experimental head trauma.
BACKGROUND
This study was designed to determine the effects of gamma-hydroxybutyric acid (GHB) on tissue lactate and malondialdehyde (MDA) levels in rabbit brain after experimental head trauma.


METHODS
Thirty New Zealand rabbits were divided equally into three groups: group S was the sham-operated group, group C, and group GHB received head trauma, where group C was the untreated and group GHB was the treated group. Head trauma was delivered by performing a craniectomy over the right hemisphere and dropping a weight of 10 g from a height of 80 cm. GHB was administered 400 mg/kg intravenously for 10 minutes after the head trauma to group GHB. The nontraumatized side was named "1" and the traumatized side was named "2." One hour after trauma, brain cortices were resected from both sides and the concentrations of lactate and MDA were determined.


RESULTS
There were significant differences between lactate and MDA levels of group S and all other groups (C1, C2, GHB1, and GHB2) except between lactate levels of group S and group GHB1, the nontraumatized and traumatized sides of groups C and group GHB, group C2 versus group GHB2, and group C1 versus group GHB1 (p < 0.05). Rectal temperature after the administration of GHB in group GHB was found lower than in groups S and C (p < 0.05).


CONCLUSIONS
These results demonstrate that head trauma leads to an increase in brain tissue lactate and MDA levels, and GHB effectively suppresses the increase of lactate and MDA.
Yosunkaya
Alper
A
Department of Anesthesiology, Faculty of Meram Medicine, University of Selçuk, Konya, Turkey. alpyos@superonline.com
Ak
Ahmet
A
Barişkaner
Hülagü
H
Ustün
Mehmet Erkan
ME
Tuncer
Sema
S
Gürbilek
Mehmet
M
D000759:Adjuvants, Anesthesia / Q000494:pharmacology*; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000293:injuries* / Q000378:metabolism; D004569:Electroencephalography / Q000187:drug effects; D005260:Female; D019344:Lactic Acid / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D011817:Rabbits; D012978:Sodium Oxybate / Q000494:pharmacology*
D000759:Adjuvants, Anesthesia; D019344:Lactic Acid; D008315:Malondialdehyde; D012978:Sodium Oxybate