[Effect of culture medium composition on callus formation and regeneration in oil flax anther culture].
Regeneration ability in callus cultures from anthers of two hybrid genotypes of oil flax was studied on N6 and LMA-1 nutrient media at various concentrations of cytokinine 6-benzylamynopurine (BAP). It was shown that callus grew and developed better at BAP concentrations of 2 mg/l, comparing with 4 and 6 mg/l. Shoot and root regeneration was observed in F1 genotype 6-8-gnezdny x M22 only and did not depend on BAP concentration in the medium and on the medium composition itself. Transfer onto fresh medium often stimulated dedifferentiation of the regenerated structures.
Soroka
A I
AI
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001593:Benzyl Compounds; D003470:Culture Media / Q000737:chemistry* / Q000494:pharmacology*; D046508:Culture Techniques; D019597:Flax / Q000196:embryology / Q000502:physiology*; D007701:Kinetin; D010937:Plant Growth Regulators / Q000494:pharmacology; D011687:Purines; D012038:Regeneration
D001593:Benzyl Compounds; D003470:Culture Media; D010937:Plant Growth Regulators; D011687:Purines; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin