title: The G protein-coupled receptor rhodopsin in the native membrane.
abstract: The higher-order structure of G protein-coupled receptors (GPCRs) in membranes may involve dimerization and formation of even larger oligomeric complexes. Here, we have investigated the organization of the prototypical GPCR rhodopsin in its native membrane by electron and atomic force microscopy (AFM). Disc membranes from mice were isolated and observed by AFM at room temperature. In all experimental conditions, rhodopsin forms structural dimers organized in paracrystalline arrays. A semi-empirical molecular model for the rhodopsin paracrystal is presented validating our previously reported results. Finally, we compare our model with other currently available models describing the supramolecular structure of GPCRs in the membrane.
authors.affiliation: M.E. Müller Institute for Microscopy, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Fotiadis
authors.affiliation: Department of Ophthalmology, University of Washington, Box 356485, Seattle, WA 98195-6485, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.affiliation: International Institute of Molecular and Cell Biology, PL-02109 Warsaw, Poland.
authors.forename: Slawomir
authors.initials: S
authors.lastname: Filipek
authors.affiliation: Department of Ophthalmology, University of Washington, Box 356485, Seattle, WA 98195-6485, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Saperstein
authors.affiliation: M.E. Müller Institute for Microscopy, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.
authors.forename: Andreas
authors.initials: A
authors.lastname: Engel
authors.affiliation: Department of Ophthalmology, University of Washington, Box 356485, Seattle, WA 98195-6485, USA.|Department of Pharmacology, University of Washington, Seattle, WA 98195, USA.|Department of Chemistry, University of Washington, Seattle, WA 98195, USA.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Palczewski
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry*; D019281:Dimerization; D051379:Mice; D018625:Microscopy, Atomic Force; D008854:Microscopy, Electron; D008958:Models, Molecular; D020836:Protein Structure, Quaternary; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure*; D012243:Rhodopsin / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure*; D012374:Rod Cell Outer Segment / Q000648:ultrastructure
chemical_list: D043562:Receptors, G-Protein-Coupled; D012243:Rhodopsin