title: ELXR: a resource for rapid exon-directed sequence analysis.
abstract: ELXR (Exon Locator and Extractor for Resequencing) streamlines the process of determining exon/intron boundaries and designing PCR and sequencing primers for high-throughput resequencing of exons. We have pre-computed ELXR primer sets for all exons identified from the human, mouse, and rat mRNA reference sequence (RefSeq) public databases curated by the National Center for Biotechnology Information. The resulting exon-flanking PCR primer pairs have been compiled into a system called ELXRdb, which may be searched by keyword, gene name or RefSeq accession number.
authors.affiliation: Eugene McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center, Harry Hines Boulevard, Dallas, TX 75390, USA. jeff.schageman@utsouthwestern.edu
authors.forename: Jeoffrey J
authors.initials: JJ
authors.lastname: Schageman
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Horton
authors.forename: Sijing
authors.initials: S
authors.lastname: Niu
authors.forename: Harold R
authors.initials: HR
authors.lastname: Garner
authors.forename: Alexander
authors.initials: A
authors.lastname: Pertsemlidis
mesh_terms: D000465:Algorithms; D000818:Animals; D019295:Computational Biology / Q000379:methods; D017931:DNA Primers / Q000235:genetics; D030541:Databases, Genetic / Q000639:trends; D005091:Exons / Q000235:genetics*; D006801:Humans; D020407:Internet; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software
chemical_list: D017931:DNA Primers