Overexpression of DRG2 increases G2/M phase cells and decreases sensitivity to nocodazole-induced apoptosis.
DRG2, a member of the DRG subfamily in the GTP-binding protein superfamily, was identified as a repressed gene product in fibroblasts transformed by SV40. The significance of this down-regulation and the cellular role of DRG2 has not been understood in the past. To investigate the function of DRG2 we made a Jurkat cell line, Jurkat-LNCX2-DRG2, stably transfected with pLNCX2-DRG2 to overexpress human DRG2. Cell cycle distribution analysis revealed an increased accumulation of G(2)/M phase cells in Jurkat-LNCX2-DRG2 cells, indicating a retardation of cell-cycle progression. In addition, an overexpression of DRG2 reduced the sensitivity of Jurkat cells to the mitotic poison nocodazole. Our data suggest that overexpression of DRG2 in Jurkat cells affects genes regulating cell-cycle arrest and apoptosis, and that these molecular changes may be important in the growth or differentiation of cells.
Song
Hebok
H
Immunomodulation Research Center, University of Ulsan, Ulsan 680-749, Korea.
Kim
Sin-Il
SI
Ko
Myoung Seok
MS
Kim
Hyo Jeong
HJ
Heo
Jin Chul
JC
Lee
Hae Jin
HJ
Lee
Han Saem
HS
Han
In Seob
IS
Kwack
KyuBum
K
Park
Jeong Woo
JW
D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D016195:G2 Phase / Q000187:drug effects; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D015739:Nocodazole / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection
C409877:DRG2 protein, human; D012333:RNA, Messenger; D019204:GTP-Binding Proteins; D015739:Nocodazole