Influences of traditional Chinese medicine on non-specific immunity of Jian Carp (Cyprinus carpio var. Jian).
The influence of traditional Chinese medicine (TCM) formulation from Astragalus Root (Radix astragalin seu Hedysari) and Chinese Angelica Root (R. Angelicae Sinensis) at a ratio of 5:1 (w/w) on non-specific immunity of Jian carp, Cyprinus carpio var. Jian was investigated. The number of NBT-positive cells in the blood and lysozyme and complement activities in the serum of Carp fed with commercial feed supplemented with 1.0% (diet 1) and 1.5% (diet 2) TCM at 10 day of post-feeding were not different from those of the control group fed with feed unsupplemented TCM 10 days post-feeding (P>0.05), but at 20 and 30 days they increased significantly (P<0.05). The values of diet 1 group and diet 2 group at 20 day and at 30 day were not significantly different (P>0.05) from each other. In addition, the TCM formula increased body weight of experimental fish by about 16.84% (diet 1) and 19% (diet 2) above that of the control group. Therefore, these data suggest that the TCM formula could elevate the function of non-specific immunity of Jian carp. The optimal dosage added to commercial carp feed was 1.0% (w/w) and the oral administration time as a course of treatment was 20 days.
Jian
Jichang
J
Fisheries College of Zhanjiang University, Xiashan District, Zhanjiang City, Guangdong 524025, China. jianjichang@21cn.com
Wu
Zaohe
Z
D029971:Angelica sinensis; D000818:Animals; D027885:Astragalus propinquus; D001835:Body Weight; D002347:Carps / Q000276:immunology*; D015941:Complement Hemolytic Activity Assay; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007113:Immunity, Innate / Q000187:drug effects*; D008516:Medicine, Chinese Traditional; D009113:Muramidase / Q000187:drug effects / Q000276:immunology; D009580:Nitroblue Tetrazolium / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D009580:Nitroblue Tetrazolium; C027492:Huang Qi; C044613:angelicae sinensis extract; D009113:Muramidase