title: Effects of Cordyceps militaris extract on angiogenesis and tumor growth.
abstract: OBJECTIVE
  To evaluate the effects of Cordyceps militaris extract (CME) on angiogenesis and tumor growth.
  
  
  METHODS
  Human umbilical vein endothelial cells (HUVEC), HT1080, and B16-F10 cells were used. DNA fragment, angiogenic related gene expressions (MMPs, bFGF, VEGF, etc), capillary tube formation, wound healing in vitro, tumor growth in vivo were measured.
  
  
  RESULTS
  CME inhibited growth of HUVECs and HT1080 (P<0.01). CME 100 and 200 mg/L reduced MMP-2 gene expression in HT1080 cells by 6.0 % and 22.9 % after 3-h and 14.9 % and 32.8 % after 6-h treatment. CME did not affect MMP-9 gene expression in B16-F10 melanoma cells. CME 100 and 200 mg/L also reduced bFGF gene expression in HUVECs by 22.2 % and 41.3 %. CME inhibited tube formation of endothelial cells in vitro and in vivo. CME repressed the growth of B16-F10 melanoma cells in mice compared with control group (P<0.05).
  
  
  CONCLUSIONS
  CME has antiangiogenetic properties.
authors.affiliation: Department of East-West Cancer Center, College of Oriental Medicine, Daejeon University, Daejeon 301-724, Korea.
authors.forename: Hwa-seung
authors.initials: HS
authors.lastname: Yoo
authors.forename: Jang-woo
authors.initials: JW
authors.lastname: Shin
authors.forename: Jung-hyo
authors.initials: JH
authors.lastname: Cho
authors.forename: Chang-gue
authors.initials: CG
authors.lastname: Son
authors.forename: Yeon-weol
authors.initials: YW
authors.lastname: Lee
authors.forename: Sang-yong
authors.initials: SY
authors.lastname: Park
authors.forename: Chong-kwan
authors.initials: CK
authors.lastname: Cho
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D032661:Cordyceps / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis* / Q000235:genetics; D005354:Fibrosarcoma / Q000473:pathology; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000235:genetics; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics; D014407:Tumor Cells, Cultured; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9