Development of a one-step immunochromatographic strip test for the detection of sennosides A and B.
An immunochromatographic strip test was developed to detect sennoside A (1) and sennoside B (2) using anti-1 and anti-2 monoclonal antibodies. The qualitative assay was based on a competitive immunoassay in which the detector reagent consisted of colloidal gold particles coated with the respective sennoside antibodies. The capture reagents were 1- and 2-human serum albumin (HSA) conjugates immobilised on a nitrocellulose membrane on the test strip. The sample containing 1 and 2, together with detector reagent, passed over the zone where the capture reagents had been immobilised. The analytes in the sample competed for binding to the limited amount of antibodies in the detector reagent with the immobilised 1- and 2-HSA conjugates on the membrane and hence positive samples showed no colour in the capture spot zone. Detection limits for the strip test were 125 ng/mL for both sennosides. The assay system is useful as a rapid and simple screening method for the detection of 1 and 2 in plants, drugs and body fluids.
Putalun
Waraporn
W
Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.
Morinaga
Osamu
O
Tanaka
Hiroyuki
H
Shoyama
Yukihiro
Y
D000880:Anthraquinones / Q000032:analysis* / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000276:immunology; D002845:Chromatography / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D007887:Fabaceae / Q000737:chemistry; D017979:Gold Colloid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000379:methods; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry; D011933:Reagent Kits, Diagnostic; D012676:Senna Extract; D000081226:Sennosides; D012680:Sensitivity and Specificity
D000880:Anthraquinones; D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D011933:Reagent Kits, Diagnostic; D000081226:Sennosides; D012676:Senna Extract