title: Detection of HIV-1 subtype G using Cobas Ampliscreen test.
abstract: A nucleic acid amplification test for detection of HIV-1 RNA was designed for screening pools of human plasma. This test achieves a similar level of sensitivity for group M subtypes, but few samples of subtype G and none of its CRFs had been tested, which are the most relevant in Portugal. We found that the test is effective in detecting HIV-1 subtypes and has an analytical sensitivity similar to B subtype.
authors.affiliation: Department of Transfusion Medicine and Blood Bank, Molecular Biology Center, Serv. Imuno-hemoterapia/Hospital S. João, Hospital S. João, 4200 Porto, Portugal. araujo@hsjoao.min-saude.pt
authors.forename: Fernando M
authors.initials: FM
authors.lastname: Araújo
authors.forename: Isabel S
authors.initials: IS
authors.lastname: Henriques
authors.forename: Fátima P
authors.initials: FP
authors.lastname: Monteiro
authors.forename: Elsa R
authors.initials: ER
authors.lastname: Meireles
authors.forename: Luis M
authors.initials: LM
authors.lastname: Cunha-Ribeiro
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis / Q000453:epidemiology; D015497:HIV-1 / Q000145:classification* / Q000302:isolation & purification; D006801:Humans; D012367:RNA, Viral / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D012367:RNA, Viral