A dendritic cell based hybrid cell vaccine generated by electrofusion for immunotherapy strategies in HNSCC.
OBJECTIVE
Hybrid cells generated from dendritic cells (DC) and tumor cells provide tumor-associated antigens (TAA) in a polyvalent mode and therefore they have aroused interest in cancer immunotherapy. The present study was designed to investigate the hybrid cell generation and optimize its implementation for a TAA-target treatment of head and neck squamous cell carcinoma (HNSCC).


METHODS
Hybrid cells from mature DC and laryngeal carcinoma cell line UTSCC-19A were generated by electrofusion. Fusion efficiency and viability were determined by flow cytometry, light and fluorescence microscopy analyses.


RESULTS
The gradual electrofusion process constituted real human tumor and dendritic cell hybrids characterized by polynuclear cells and double staining as a result of overlay of red (HLA-DR:R-PE) and green (HEA:FITC) fluorescence. Furthermore, analyses have proven viability of fusion results, and factors influencing fusion yield were determined.


CONCLUSIONS
Physical fusion of mature dendritic cells with laryngeal carcinoma cells provides a dendritic cell based hybrid cell vaccine as a quantitative prerequisite for anti-cancer vaccination. Specific cytotoxic T-lymphocytes need to be induced before hybrid cell application in clinical studies.
Weise
Jan Bernd
JB
Department of Otorhinolaryngology, Head and Neck Surgery, University of Kiel, Arnold-Heller-Strasse 14, D-24105 Kiel, Germany. jweise@hno.uni-kiel.de
Maune
Steffen
S
Görögh
Tibor
T
Kabelitz
Dietrich
D
Arnold
Norbert
N
Pfisterer
Jacobus
J
Hilpert
Felix
F
Heiser
Axel
A
D000951:Antigens, Neoplasm / Q000276:immunology* / Q000627:therapeutic use; D019496:Cancer Vaccines / Q000627:therapeutic use*; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000628:therapy*; D002459:Cell Fusion / Q000379:methods; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D005434:Flow Cytometry; D006258:Head and Neck Neoplasms / Q000276:immunology / Q000628:therapy*; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology*; D016130:Immunophenotyping; D007167:Immunotherapy / Q000379:methods; D008856:Microscopy, Fluorescence
D000951:Antigens, Neoplasm; D019496:Cancer Vaccines