title: Genealogical concordance between the mating type locus and seven other nuclear genes supports formal recognition of nine phylogenetically distinct species within the Fusarium graminearum clade.
abstract: Species limits were investigated within the Fusarium graminearum clade (Fg clade) through phylogenetic analyses of DNA sequences from portions of 11 nuclear genes including the mating-type (MAT) locus. Nine phylogenetically distinct species were resolved within the Fg clade, and they all possess contiguous MAT1-1 and MAT1-2 idiomorphs consistent with a homothallic reproductive mode. In contrast, only one of the two MAT idiomorphs was found in five other species, four of which were putatively asexual, and the other was heterothallic. Molecular evolutionary analyses indicate the MAT genes are under strong purifying selection and that they are functionally constrained, even in species for which a sexual state is unknown. The phylogeny supports a monophyletic and apomorphic origin of homothallism within this clade. Morphological analyses demonstrate that a combination of conidial characters could be used to differentiate three species and three species pairs. Species rank is formally proposed for the eight unnamed species within the Fg clade using fixed nucleotide characters.
authors.affiliation: National Center for Agricultural Utilization Research, US Department of Agriculture, Agricultural Research Service, Peoria, IL 61604, USA. kodonnell@ncaur.usda.gov
authors.forename: Kerry
authors.initials: K
authors.lastname: O'Donnell
authors.forename: Todd J
authors.initials: TJ
authors.lastname: Ward
authors.forename: David M
authors.initials: DM
authors.lastname: Geiser
authors.forename: H
authors.initials: H
authors.lastname: Corby Kistler
authors.forename: Takayuki
authors.initials: T
authors.lastname: Aoki
mesh_terms: D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000235:genetics; D004271:DNA, Fungal / Q000032:analysis; D019143:Evolution, Molecular; D005656:Fungal Proteins / Q000235:genetics*; D005670:Fusarium / Q000145:classification* / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics*; D049770:Genes, Mating Type, Fungal; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins