title: Methionine synthase reductase MTRR 66A > G has no effect on total homocysteine, folate, and Vitamin B12 concentrations in renal transplant patients.
abstract: The association of variants of the gene encoding methionine synthase reductase (MTRR) with hyperhomocysteinemia, folate and Vitamin B(12) status in kidney graft recipients is unknown. We examined two mutations in MTRR in a cross-sectional study of 733 kidney graft recipients. The allele frequency of MTRR 66G was 0.55. 369 patients (50.3%) were heterozygous and 219 patients (29.9%) were homozygous for the mutation. None of the patients showed the 997C > G mutation. The allelic variants of MTRR 66A > G showed no significant association with total homocysteine (tHcy) levels, both in univariate analyses, and in a multivariate model controlling for age, gender, body mass index, renal function, time since transplantation, underlying kidney disease, as well as the MTHFR 677C > T/1298A > C genotypes. Similarly, no significant associations between the MTRR 66A > Ggenotypes and plasma folate or Vitamin B(12) levels were found. In conclusion, MTRR 66A > G has no major effect on tHcy, folate, or Vitamin B(12) plasma concentrations in kidney graft recipients.
authors.affiliation: Division of Nephrology and Dialysis, Department of Medicine III, University of Vienna, Währinger Gürtel 18-20, 1090 Vienna, Austria. alexandrafeix@hotmail.com
authors.forename: Alexandra
authors.initials: A
authors.lastname: Feix
authors.forename: Wolfgang C
authors.initials: WC
authors.lastname: Winkelmayer
authors.forename: Corinna
authors.initials: C
authors.lastname: Eberle
authors.forename: Gere
authors.initials: G
authors.lastname: Sunder-Plassmann
authors.forename: Manuela
authors.initials: M
authors.lastname: Födinger
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D001483:Base Sequence; D003430:Cross-Sectional Studies; D005260:Female; D005287:Ferredoxin-NADP Reductase / Q000235:genetics*; D005492:Folic Acid / Q000032:analysis / Q000378:metabolism*; D015870:Gene Expression; D006084:Graft Rejection; D006085:Graft Survival; D006710:Homocysteine / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000175:diagnosis / Q000601:surgery; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D015999:Multivariate Analysis; D009154:Mutation; D016133:Polymerase Chain Reaction; D011379:Prognosis; D014805:Vitamin B 12 / Q000032:analysis / Q000378:metabolism*
chemical_list: D006710:Homocysteine; D005492:Folic Acid; C111241:methionine synthase reductase; D005287:Ferredoxin-NADP Reductase; D014805:Vitamin B 12