DNA vaccines against the human papillomavirus type 16 E6 or E7 oncoproteins.
DNA vaccines expressing the E6 or E7 oncoproteins of human papilloma virus type 16 (HPV-16) in either their wild-type form or fused to sequences that affect intracellular trafficking were tested for induction of protective immunity against tumor cell challenge in two models based on BALB/c and C57Bl/6 mice. The DNA vaccines to E7 gave uniformly disappointing results, while the DNA vaccine that expressed E6 linked to a viral leader sequence protected BALB/c mice against tumor cell challenge given before or after vaccination. The efficacy of this vaccine could be enhanced by a DNA vector prime/viral vector boost regimen. In contrast, priming of mice with the DNA vaccines to E7 reduced the efficacy of a viral vector expressing the same antigen.
Wlazlo
Anthony P
AP
The Wistar Institute, 3601 Spruce St., Philadelphia, Pennsylvania 19104, USA.
Deng
Hongying
H
Giles-Davis
Wynetta
W
Ertl
Hildegund C J
HC
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D019556:COS Cells; D019496:Cancer Vaccines; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis; D004247:DNA / Q000378:metabolism; D005822:Genetic Vectors; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000517:prevention & control* / Q000628:therapy*; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D050725:Papillomavirus E7 Proteins; D053918:Papillomavirus Vaccines; D010455:Peptides / Q000737:chemistry; D012097:Repressor Proteins / Q000235:genetics*; D013154:Spleen / Q000166:cytology; D013997:Time Factors; D019444:Vaccines, DNA
D019496:Cancer Vaccines; D016207:Cytokines; C068651:E6 protein, Human papillomavirus type 16; D007376:Interleukin-2; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D053918:Papillomavirus Vaccines; D010455:Peptides; D012097:Repressor Proteins; D019444:Vaccines, DNA; C059731:oncogene protein E7, Human papillomavirus type 16; D004247:DNA