title: High mobility group A1 expression correlates with the histological grade of human glial tumors.
abstract: Glioblastoma is one of the most aggressive tumors in mankind with 50% of patients dying within the 1st year of diagnosis, and being refractory to conventional therapies. The aim of our work has been to analyse the expression of the HMGA1 proteins in human astrocytomas and glioblastomas in order to verify whether the detection of these proteins might be of some help in the diagnosis of these neoplasias. Here we report the analysis of 27 cases, including 12 astrocytomas and 15 glioblastomas, for HMGA1 expression. All the neoplastic samples showed positive staining even though the number of positive cells and the staining intensity was higher in glioblastomas compared to astrocytomas. Conversely, HMGA1 proteins were not detected in normal brain. Accordingly, expression of the hmga1 gene, analysed by RT-PCR, was higher in glioblastomas than in astrocytomas.
authors.affiliation: Cattedra-Servizio di Anatomia Patologica, Facoltà di Medicina e Chirurgia Università 'Magna Graecia', 88100 Catanzaro, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Donato
authors.forename: Josefina
authors.initials: J
authors.lastname: Martinez Hoyos
authors.forename: Andrea
authors.initials: A
authors.lastname: Amorosi
authors.forename: Lorenza
authors.initials: L
authors.lastname: Maltese
authors.forename: Angelo
authors.initials: A
authors.lastname: Lavano
authors.forename: Giorgio
authors.initials: G
authors.lastname: Volpentesta
authors.forename: Francesco
authors.initials: F
authors.lastname: Signorelli
authors.forename: Francesca
authors.initials: F
authors.lastname: Pentimalli
authors.forename: Pierlorenzo
authors.initials: P
authors.lastname: Pallante
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Ferraro
authors.forename: Luigi
authors.initials: L
authors.lastname: Tucci
authors.forename: Cosma Damiano
authors.initials: CD
authors.lastname: Signorelli
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Viglietto
authors.forename: Alfredo
authors.initials: A
authors.lastname: Fusco
mesh_terms: D000328:Adult; D001254:Astrocytoma / Q000175:diagnosis / Q000378:metabolism*; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000175:diagnosis / Q000378:metabolism*; D025741:HMGA1a Protein / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D025741:HMGA1a Protein