title: In vivo epinephrine-mediated regulation of gene expression in human skeletal muscle.
abstract: The stress hormone epinephrine produces major physiological effects on skeletal muscle. Here we determined skeletal muscle mRNA expression profiles before and during a 6-h epinephrine infusion performed in nine young men. Stringent statistical analysis of data obtained using 43000 cDNA element microarrays showed that 1206 and 474 genes were up- and down-regulated, respectively. Microarray data were validated using reverse transcription quantitative PCR. Gene classification was performed through data mining of Gene Ontology annotations, cluster analysis of regulated genes among 14 human tissues, and correlation analysis of mRNA and clinical parameter variations. Evidence of an autoregulatory control was provided by the regulation of key genes of the cAMP-dependent transcription pathway. Genes with known functional cAMP response elements were regulated by the hormone. The impact on metabolism was illustrated by coordinated regulations of genes involved in carbohydrate and protein metabolisms. Epinephrine had a profound effect on genes involved in immunity and inflammatory response, a previously unappreciated aspect of catecholamine action. Information on 526 mRNAs corresponded to genes of unknown function. These data define the molecular signatures of epinephrine action in human skeletal muscle. They may contribute to the understanding of skeletal muscle alterations observed in pathological conditions characterized by sympathetic nervous system overdrive.
authors.affiliation: Unité de Recherches sur les Obésités, Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 586, Institut Louis Bugnard, Centre Hospitalier Universitaire de Toulouse, Université Paul Sabatier, 31403 Toulouse, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Viguerie
authors.forename: Karine
authors.initials: K
authors.lastname: Clement
authors.forename: Pierre
authors.initials: P
authors.lastname: Barbe
authors.forename: Melanie
authors.initials: M
authors.lastname: Courtine
authors.forename: Arriel
authors.initials: A
authors.lastname: Benis
authors.forename: Dominique
authors.initials: D
authors.lastname: Larrouy
authors.forename: Blaise
authors.initials: B
authors.lastname: Hanczar
authors.forename: Veronique
authors.initials: V
authors.lastname: Pelloux
authors.forename: Christine
authors.initials: C
authors.lastname: Poitou
authors.forename: Yadh
authors.initials: Y
authors.lastname: Khalfallah
authors.forename: Gregory S
authors.initials: GS
authors.lastname: Barsh
authors.forename: Claire
authors.initials: C
authors.lastname: Thalamas
authors.forename: Jean-Daniel
authors.initials: JD
authors.lastname: Zucker
authors.forename: Dominique
authors.initials: D
authors.lastname: Langin
mesh_terms: D000322:Adrenergic Agonists / Q000378:metabolism* / Q000494:pharmacology; D000328:Adult; D002320:Cardiovascular Physiological Phenomena; D000242:Cyclic AMP / Q000378:metabolism; D017362:Cyclic AMP Response Element-Binding Protein / Q000502:physiology; D004734:Energy Metabolism / Q000502:physiology; D004837:Epinephrine / Q000494:pharmacology / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008297:Male; D008660:Metabolism / Q000502:physiology; D018482:Muscle, Skeletal / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D013312:Stress, Physiological / Q000235:genetics; D015854:Up-Regulation
chemical_list: D000322:Adrenergic Agonists; D017362:Cyclic AMP Response Element-Binding Protein; D011506:Proteins; D012333:RNA, Messenger; D000242:Cyclic AMP; D004837:Epinephrine