title: [From the conception of the PRINS to its coronation].
abstract: As a non-isotopic molecular cytogenetic technique, the primed in situ (PRINS) labelling reaction represents a major technological progress achieved in the past decade. It has become a routine technique for the microscopic visualization of specific DNA sequences in cells and nuclei and constitutes a good alternative to the fluorescence in situ hybridization (FISH) procedure. Among the multiple advantages that characterize the PRINS technique, specificity, rapidity, reliability, reproducibility, and cost-effectiveness can be mentioned. PRINS can be in addition associated with other techniques like FISH, indirect immunofluorescence, and nick translation. The most recent developments show the great potential of this technique. Now PRINS can be used to study single-copy genes and, consequently, can be routinely used to investigate deletions associated with microdeletion syndromes. Therefore, the PRINS technique has the potential to become a widely used molecular cytogenetic tool in clinics and research. This short review presents how the PRINS technique contributed to further the understanding of biological phenomena and describes the different possibilities and applications of the PRINS method in several biological and clinical fields (pre-implantation testing, prenatal, constitutional and oncologic genetic diagnosis).
authors.affiliation: Département de Pédiatrie, Faculté de médecine, Université de Sherbrooke, Québec, Canada.
authors.forename: Kada
authors.initials: K
authors.lastname: Krabchi
authors.forename: Josée
authors.initials: J
authors.lastname: Lavoie
authors.forename: Philippe
authors.initials: P
authors.lastname: Coullin
authors.forename: Marc
authors.initials: M
authors.lastname: Bronsard
authors.forename: Franck
authors.initials: F
authors.lastname: Pellestor
authors.forename: Ju
authors.initials: J
authors.lastname: Yan
authors.forename: Régen
authors.initials: R
authors.lastname: Drouin
mesh_terms: D001483:Base Sequence; D002875:Chromosomes / Q000235:genetics / Q000648:ultrastructure; D004247:DNA / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D005315:Fetal Diseases / Q000175:diagnosis / Q000235:genetics; D005455:Fluorescent Antibody Technique; D030342:Genetic Diseases, Inborn / Q000175:diagnosis / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D008677:Metaphase; D019836:Preimplantation Diagnosis / Q000379:methods; D011296:Prenatal Diagnosis / Q000379:methods; D020401:Primed In Situ Labeling / Q000379:methods / Q000639:trends; D012680:Sensitivity and Specificity
chemical_list: D004273:DNA, Neoplasm; D004279:DNA, Viral; D004247:DNA