Gene expression analysis in Interleukin-12-induced suppression of mouse mammary carcinoma.
Interleukin-12 (IL-12) has potent antitumor activities via natural killer cells and cytotoxic T lymphocytes. However, the molecular mechanisms whereby IL-12 induces tumoricidal activities are poorly understood. Here, we report the genome-wide analysis of gene expression in a primary murine mammary carcinoma model that resembles human breast cancer, following the therapeutic application of recombinant IL-12, which restricted tumor growth and metastasis. IL-12 was able to curtail neovascularization in the tumor as well as enhance the number of tumor-infiltrating lymphocytes. Comprehensive examination of global gene expression revealed IL-12-induced molecular changes associated with tumor regression and reduced lung metastasis, thus providing a high-resolution snapshot of a host response against a developing malignancy and a rich source of potential targets for therapeutic intervention of breast cancer.
Shi
Xiaoyan
X
Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, N Y10021, USA.
Liu
Jianguo
J
Xiang
Zhaoying
Z
Mitsuhashi
Maki
M
Wu
Rita S
RS
Ma
Xiaojing
X
D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D018664:Interleukin-12 / Q000627:therapeutic use*; D016246:Lymphocytes, Tumor-Infiltrating / Q000187:drug effects; D008325:Mammary Neoplasms, Experimental / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009389:Neovascularization, Pathologic / Q000517:prevention & control; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018664:Interleukin-12