title: Dynamic contrast-enhanced magnetic resonance imaging at 1.5 Tesla with gadopentetate dimeglumine to assess the angiostatic effects of anginex in mice.
abstract: The purpose of this study was to evaluate the effects of anginex on tumour angiogenesis assessed by dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) on a clinical 1.5 Tesla MR system and with the clinically available contrast agent gadopentetate dimeglumine. C57BL/6 mice carrying B16F10 melanomas were treated with anginex, TNP-470 or saline. Tumour growth curves and microvessel density (MVD) were recorded to establish the effects of treatment. DCE-MRI was performed on day 16 after tumour inoculation, and the endothelial transfer coefficients of the microvessel permeability surface-area product (K(PS)) were calculated using a two-compartment model. Both anginex and TNP-470 resulted in smaller tumour volumes (P<0.0001) and lower MVD (P <0.05) compared to saline. Treatment with anginex resulted in a 64% reduction (P<0.01) of tumour K(PS) and TNP-470 resulted in a 44% reduction (P=0.17), compared to saline. DCE-MRI with a clinically available, small-molecular contrast agent can therefore be used to evaluate the angiostatic effects of anginex and TNP-470 on tumour angiogenesis.
authors.affiliation: Department of Radiology, Maastricht University Hospital, and Cardiovascular Research Institute Maastricht, Maastricht University, The Netherlands.
authors.forename: Q G
authors.initials: QG
authors.lastname: de Lussanet
authors.forename: R G H
authors.initials: RG
authors.lastname: Beets-Tan
authors.forename: W H
authors.initials: WH
authors.lastname: Backes
authors.forename: D W J
authors.initials: DW
authors.lastname: van der Schaft
authors.forename: J M A
authors.initials: JM
authors.lastname: van Engelshoven
authors.forename: K H
authors.initials: KH
authors.lastname: Mayo
authors.forename: A W
authors.initials: AW
authors.lastname: Griffioen
mesh_terms: D020533:Angiogenesis Inhibitors / Q000627:therapeutic use*; D000818:Animals; D003287:Contrast Media; D019786:Gadolinium DTPA; D008279:Magnetic Resonance Imaging / Q000379:methods; D008545:Melanoma / Q000098:blood supply* / Q000188:drug therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D009389:Neovascularization, Pathologic / Q000175:diagnosis / Q000517:prevention & control*; D010455:Peptides; D011506:Proteins
chemical_list: D020533:Angiogenesis Inhibitors; D003287:Contrast Media; D010455:Peptides; D011506:Proteins; C111993:betapep-25 protein, synthetic; D019786:Gadolinium DTPA