[The effect of newcastle disease virus on the biological behavior of tumor cells].
OBJECTIVE
To investigate the anti-tumor effect of newcastle disease virus (NDV).


METHODS
Plaque formation test was used to investigate the effect of NDV on chicken embryofibroblasts(CEF). Cell suppression test, agarose gel electrophoresis, cytoskeleton staining, fluorescence staining, TUNEL staining, and sialic acid content determination were used to observe the influence of NDV on several human tumor cells.


RESULTS
The Plaque formation was observed in chicken embryo fibroblasts.NDV could lead to the apparent cytopathy of BHK, Hela and Hep-2 tumor cells, but has no apparent effect on Wish cells. The strong suppressive effect of NDV on the growth of these tumor cells was found without dose dependence. The optimal dose of NDV could induce the death of tumor cells which was mainly apoptosis, as showed by the classical DNA ladder in DNA gel electrophoresis. NDV also resulted in the changes of cytoskeleton and decreased the level of sialic acid contents on tumor cells.


CONCLUSIONS
NDV may be a potential anti-tumor agent.
Xue
Li-juan
LJ
Quartermaster University of Chinese PLA, Changchun 130062, China.
Jin
Ning-yi
NY
Gong
Wei
W
Wang
Hong-wei
HW
Sun
Da-hui
DH
Luo
Qin-fang
QF
Ge
Tao
T
Li
Ping
P
D000818:Animals; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D016923:Cell Death; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002478:Cells, Cultured; D002642:Chick Embryo; D006224:Cricetinae; D003599:Cytoskeleton / Q000821:virology; D005347:Fibroblasts / Q000166:cytology / Q000821:virology; D006367:HeLa Cells; D006801:Humans; D007668:Kidney / Q000166:cytology; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D009522:Newcastle disease virus / Q000472:pathogenicity / Q000502:physiology*; D010948:Viral Plaque Assay
D019158:N-Acetylneuraminic Acid