title: Nucleosides and ODN electrochemical detection onto boron doped diamond electrodes.
abstract: Boron doped diamond (BDD) is a promising material for electroanalytical chemistry due mainly to its chemical stability, its high electrical conductivity and to the large amplitude of its electroactive window in aqueous media. The latter feature allowed us to study the direct oxidation of the two electroactive nucleosides, guanosine and adenosine. The BDD electrode was first activated by applying high oxidizing potentials, allowing to increase anodically its working potential window through the oxidation of CH surface groups into hydroxyl and carbonyl terminations. Guanosine (1.2 V vs. Ag/AgCl) and adenosine (1.5 V vs. Ag/AgCl) could then be detected electrochemically with an acceptable signal to noise ratio. The electrochemical signature of each oxidizable base was assessed using differential pulse voltammetry (DPV), in solutions containing one or both nucleosides. These experiments pointed out the existence of adsorption phenomena of the oxidized products onto the diamond surface. Scanning electrochemical microscopy (SECM) was used to investigate these adsorption effects at the microscopic scale. The usefulness of BDD electrodes for the direct electrochemical detection of synthetic oligonucleotides is also evidenced.
authors.affiliation: CREAB group, Laboratoire des Structures et Propriétés des Architectures Moléculaires UMR 5819 CNRS-CEA-Université Joseph Fourier Grenoble 1, DRFMC/SI3M/CREAB, CEA 17 avenue des Martyrs 38054 Grenoble cedex 9, France. pmailley@cea.fr
authors.forename: Elodie
authors.initials: E
authors.lastname: Fortin
authors.forename: Jérôme
authors.initials: J
authors.lastname: Chane-Tune
authors.forename: Pascal
authors.initials: P
authors.lastname: Mailley
authors.forename: Sabine
authors.initials: S
authors.lastname: Szunerits
authors.forename: Bernadette
authors.initials: B
authors.lastname: Marcus
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Petit
authors.forename: Michel
authors.initials: M
authors.lastname: Mermoux
authors.forename: Eric
authors.initials: E
authors.lastname: Vieil
mesh_terms: D000241:Adenosine / Q000032:analysis* / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D001895:Boron / Q000737:chemistry*; D018130:Diamond / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D006151:Guanosine / Q000032:analysis* / Q000737:chemistry; D009705:Nucleosides / Q000032:analysis / Q000737:chemistry; D009841:Oligonucleotides / Q000032:analysis* / Q000737:chemistry; D010084:Oxidation-Reduction
chemical_list: D009705:Nucleosides; D009841:Oligonucleotides; D006151:Guanosine; D018130:Diamond; D000241:Adenosine; D001895:Boron