Anti-human Olig2 antibody as a useful immunohistochemical marker of normal oligodendrocytes and gliomas.
Olig2 is a recently identified transcription factor involved in the phenotype definition of cells in the oligodendroglial lineage. The expression of Olig2 transcript has been demonstrated in human oligodendroglial tumors, although the protein expression has not been studied extensively. We developed a polyclonal antibody to human Olig2 and analyzed it immunohistochemically. The antibody depicted a single distinct band of predicted molecular weight by Western blotting, and did not cross-react with human Olig1. In normal human brain tissue, the nuclei of oligodendrocytes of interfascicular, perivascular, and perineuronal disposition were clearly labeled by the antibody. Similarly, the nuclei of oligodendroglial tumors were labeled. There was no apparent correlation between the staining intensity and histological grade. Astrocytic components within the tumors were generally less or not stained. Astrocytic tumors were also positive with the Olig2 antiserum to a lesser extent, and the difference between oligodendroglial and astrocytic tumors was demonstrated by a statistical analysis. Olig2 and glial fibrillary acidic protein were expressed in a mutually exclusive manner, and Olig2 expression was cell-cycle related. Neither central neurocytoma nor schwannoma cases were stained. Our antibody was demonstrated to be useful in recognizing normal oligodendrocytes on paraffin sections, and applicable in diagnosis of some brain tumors.
Yokoo
Hideaki
H
Department of Pathology, Gunma University Graduate School of Medicine, Maebashi, Japan. yokoo@med.gunma-u.ac.jp
Nobusawa
Sumihito
S
Takebayashi
Hirohide
H
Ikenaka
Kazuhiro
K
Isoda
Koji
K
Kamiya
Makoto
M
Sasaki
Atsushi
A
Hirato
Junko
J
Nakazato
Yoichi
Y
D000818:Animals; D000906:Antibodies / Q000737:chemistry; D051792:Basic Helix-Loop-Helix Proteins; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002453:Cell Cycle; D019070:Cell Lineage; D002467:Cell Nucleus / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005910:Glioma / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D051379:Mice; D041681:NIH 3T3 Cells; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000276:immunology; D000076104:Oligodendrocyte Transcription Factor 2; D009836:Oligodendroglia / Q000276:immunology / Q000473:pathology*; D010232:Paraffin / Q000378:metabolism
D000906:Antibodies; D051792:Basic Helix-Loop-Helix Proteins; D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; D000076104:Oligodendrocyte Transcription Factor 2; D010232:Paraffin; C405708:OLIG2 protein, human; C512773:Olig2 protein, mouse