title: Gene content of the 750-kb critical region for mouse embryonic ectoderm lethal tcl-w5.
abstract: Mice homozygous for the t(w5) allele arrest at gastrulation from defects associated with embryonic ectoderm development. The mutated gene has been genetically closely linked to the H-2K locus in the mouse MHC region, flanked by markers H-2Pb and D17Mit147. Aiming at the positional cloning of the mutated gene, we constructed a BAC contig spanning about 1 Mb of the genomic region. On the basis of our mapping and sequencing analysis of the BACs combined with public genome data, EST database searches, and gene prediction programs, we delimit the 1.06 cM of the t(w5) critical region to 750 kb, and infer 36 genes (1/20 kb) encoded in the interval. All of the 33 genes tested were confirmed as expressed in embryonic tissues by RT-PCR analyses, and in many cases by EST expression profiles as well. Thus, this highly gene-rich region is essentially totally transcribed during early development and provides priority candidates to be screened for the t(w5) embryonic lesion.
authors.affiliation: Technology and Development Team for Mammalian Cellular Dynamics, BioResource Center, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan. abe@rtc.riken.go.jp
authors.forename: Kuniya
authors.initials: K
authors.lastname: Abe
authors.forename: Misako
authors.initials: M
authors.lastname: Yuzuriha
authors.forename: Michihiko
authors.initials: M
authors.lastname: Sugimoto
authors.forename: Minoru S H
authors.initials: MS
authors.lastname: Ko
authors.forename: Mgavi
authors.initials: M
authors.lastname: Brathwaite
authors.forename: Paul
authors.initials: P
authors.lastname: Waeltz
authors.forename: Ramaiah
authors.initials: R
authors.lastname: Nagaraja
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping / Q000662:veterinary; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D004475:Ectoderm; D020224:Expressed Sequence Tags; D005805:Genes, MHC Class I / Q000235:genetics*; D005819:Genetic Markers; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D005819:Genetic Markers; D012313:RNA