Overexpression of the erbB-2 proto-oncogene in canine osteosarcoma cell lines and tumors.
The status of the erbB-2 (human epidermal growth factor receptor 2/neu) proto-oncogene in canine osteosarcoma (OSA) has not been reported previously. In this study we used real-time reverse transcriptase polymerase chain reaction to evaluate erbB-2 expression in seven canine OSA cell lines and 10 canine OSA tissue samples. We determined erbB-2 to be significantly overexpressed in 86% (six of seven) of the cell lines and 40% (4 of 10) of the OSA tissues samples. Given the importance of erbB-2 in human breast cancer, the finding of erbB-2 overexpression in canine OSA may be important in further understanding the pathogenesis and possible therapies of OSA.
Flint
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AF
Department of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Campus Delivery, Building Physiology 1680, Fort Collins, CO 80523, USA.
U'Ren
L
L
Legare
M E
ME
Withrow
S J
SJ
Dernell
W
W
Hanneman
W H
WH
D000818:Animals; D045744:Cell Line, Tumor; D017931:DNA Primers; D004195:Disease Models, Animal; D004285:Dogs; D015972:Gene Expression Regulation, Neoplastic; D018734:Genes, erbB-2; D006652:Histological Techniques; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology; D012516:Osteosarcoma / Q000378:metabolism* / Q000473:pathology; D000090063:Proto-Oncogene Mas; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D000090063:Proto-Oncogene Mas; D018719:Erb-b2 Receptor Tyrosine Kinases; C000715470:MAS1 protein, human