An alternative and simple method to consistently prepare viable isolated human islets for clinical transplantation.
We describe a method to consistently prepare human islets for transplantation. By combining a simple collagenase digestion method and a density gradient purification system, we were able to obtain successful isolations (>/=200,000 islet equivalents, >/=50% purity) in 69% of processed glands. No reagent of animal source was used. Isolated islets were morphologically well maintained and functionally competent, with sterility confirmed in 97% of cases. Two patients were transplanted with islets prepared by this method; graft function was demonstrated for a few months. Improved simplicity and consistency, together with adequate quality of the preparations, are the main features of this isolation method.
Bugliani
M
M
Department of Endocrinology and Metabolism, Metabolic Unit, University of Pisa, Pisa, Italy.
Lupi
R
R
Del Guerra
S
S
Boggi
U
U
Marselli
L
L
Sbrana
S
S
Vistoli
F
F
Torri
S
S
Del Chiaro
M
M
Signori
S
S
Filipponi
F
F
Del Prato
S
S
Campa
M
M
Corsini
V
V
Campatelli
A
A
Di Candio
F
F
Mosca
F
F
Marchetti
P
P
D000328:Adult; D002469:Cell Separation / Q000379:methods; D006085:Graft Survival; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology*; D016381:Islets of Langerhans Transplantation / Q000379:methods* / Q000502:physiology; D008875:Middle Aged; D009926:Organ Preservation / Q000379:methods; D014019:Tissue Donors / Q000706:statistics & numerical data; D020858:Tissue and Organ Harvesting / Q000379:methods; D016896:Treatment Outcome