title: BF, HP, DQB and DRB are associated with haemolytic complement activity, acute phase protein reaction and antibody response in the pig.
abstract: In order to examine the loci factor B (BF), C3, corticotropin releasing hormone (CRH), DQB, DRB, haptoglobin (HP) and transforming growth factor beta 1 (TGFB1) for association with traits of humoral, specific and unspecific defence F2-animals of a porcine resource family were genotyped at single nucleotide polymorphisms within these loci. Haemolytic complement activity in the alternative and classical pathway, C3c and haptoglobin serum concentration and antibody titres were determined immediately prior and at days 4 and 10 after vaccinations against Mycoplasma hyopneumoniae (Mh), Aujeszky's disease virus, and porcine reproductive and respiratory syndrome virus at 6, 14 and 16 weeks of age, respectively. Analysis of variance revealed association of BF, HP and DRB with C3c serum concentration. The trend of haemolytic complement activity and C3c serum concentration during the experiment was affected by the interaction of DQB genotype and time of measurement. Association with antibody titres were found for BF, DQB and DRB. Results of the mixed model analyses were confirmed by quantitative transmission disequilibrium test that showed linkage and association with antibody titres, complement activity and acute phase reaction at certain times of measurement. The findings promote the importance of the candidate genes for humoral mechanisms of unspecific and specific defence that provide natural resistance against many pathogens.
authors.affiliation: Institute of Animal Breeding and Genetics, University of Bonn, Endenicher Allee 15, 53115 Bonn, Germany. wimmers@fbn-dummerstorf.de
authors.forename: Klaus
authors.initials: K
authors.lastname: Wimmers
authors.forename: Karl
authors.initials: K
authors.lastname: Schellander
authors.forename: Siriluck
authors.initials: S
authors.lastname: Ponsuksili
mesh_terms: D000209:Acute-Phase Proteins / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D011415:Complement Factor B / Q000235:genetics / Q000276:immunology; D003165:Complement System Proteins / Q000235:genetics / Q000276:immunology*; D003346:Corticotropin-Releasing Hormone / Q000235:genetics / Q000276:immunology; D003433:Crosses, Genetic; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics / Q000276:immunology; D059866:HLA-DQ beta-Chains; D006242:Haptoglobins / Q000235:genetics / Q000276:immunology; D011558:Herpesvirus 1, Suid / Q000276:immunology; D008297:Male; D045705:Mycoplasma hyopneumoniae / Q000276:immunology; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology; D040641:Quantitative Trait Loci / Q000235:genetics / Q000276:immunology; D013552:Swine / Q000235:genetics / Q000276:immunology*; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000276:immunology; D014611:Vaccination / Q000662:veterinary
chemical_list: D000209:Acute-Phase Proteins; D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C057615:HLA-DQbeta antigen; D006242:Haptoglobins; D016212:Transforming Growth Factor beta; D003165:Complement System Proteins; D003346:Corticotropin-Releasing Hormone; D011415:Complement Factor B