[Mutation of Drosophila melanogaster glass-like suppress expression of mini-white transgenes and dependence on their genomic environment].
Pleiotropic recessive mutation glass-like (gl-l) found in region 8C-10E of the X chromosome was shown to cause glass-like eyes having no boundaries between facets and a nonuniform pigment distribution in the presence of the endogenous white gene. The gl-l mutation completely inhibited expression of the mini-white transgene contained in several constructs, but the effect depended on the site of construct integration in the genome. The mutation had no effect on the expression of the white transgene having the enhancer and flanked by insulators. The gl-l mutation did not affect the extent of mosaic eye pigmentation when a construct with mini-white was inserted in the telomeric or pericentric region. However, in most cases it completely inhibited the mosaic mini-white expression when cloned heterochromatic repeats were adjacent to the reporter gene in a construct. The gl-l gene was assumed to play a role in the formation of the chromatin structure, because the effect of its mutation on expression of the white transgene depended on the transgene insertion site, the presence of insulators or an enhancer in the vicinity of the transgene, and on the adjacent heterochromatic repeats.
Popkova
A M
AM
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182 Russia.
Abramov
Iu A
IuA
Gvozdev
V A
VA
D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000818:Animals; D003001:Cloning, Molecular; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics*; D005136:Eye Proteins / Q000235:genetics; D016678:Genome; D006570:Heterochromatin / Q000235:genetics; D019076:Transgenes
D018528:ATP-Binding Cassette Transporters; D029721:Drosophila Proteins; D005136:Eye Proteins; D006570:Heterochromatin; C059254:w protein, Drosophila