Acetyl coenzyme A concentrations in plant tissues.
Despite the importance of acetyl coenzyme A in many facets of metabolism and the availability of methods for estimation of its concentration, data for acetyl-CoA concentrations in plant tissues have been very scarce. A method using reversed phase HPLC for the quantitative estimation of acetyl-CoA was applied to a variety of plant tissues. In three different developing oilseeds the bulk acetyl-CoA concentration ranged from 5 to 25 nmoles/g fresh weight. In Arabidopsis thaliana leaves it was 5 nmoles/g fresh weight, and in Spinacia oleracea leaves 6.8 nmoles/g fresh weight. Immediate quenching of the harvested tissue in liquid nitrogen is needed to obtain high recoveries of acetyl-CoA.
Tumaney
Ajay W
AW
Department of Plant Biology, Michigan State University, East Lansing, MI 48824, USA.
Ohlrogge
John B
JB
Pollard
Mike
M
D000105:Acetyl Coenzyme A / Q000096:biosynthesis*; D017360:Arabidopsis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D018515:Plant Leaves / Q000378:metabolism; D010944:Plants / Q000378:metabolism*; D018724:Spinacia oleracea / Q000378:metabolism
D000105:Acetyl Coenzyme A