title: Hunting for genes by functional screens.
abstract: Advances in high throughput sequencing technologies have led to an explosion of sequence information available for today's researchers. Efforts in the emerging next phase of the genomic era are focusing on the assignment of function to genes uncovered by genome sequencing programs. The main approaches include high throughput mutagenesis, predictions based on homology in primary sequence, microarray and proteomics. Despite the variety of strategies applied, only 30% of predicted human genes have any function assigned. There is a need, therefore, for additional tools to overcome some of the limitations of existing techniques. In this review we discuss some recent developments and their impact on gene function annotation, especially as they relate to the elucidation of signalling cascades activated by cytokines and growth factors.
authors.affiliation: Cardiovascular Research Unit, Division of Clinical Sciences (North), University of Sheffield, Northern General Hospital, Sheffield S5 7AU, UK. e.kiss-toth@sheffield.ac.uk
authors.forename: Endre
authors.initials: E
authors.lastname: Kiss-Toth
authors.forename: Eva E
authors.initials: EE
authors.lastname: Qwarnstrom
authors.forename: Steven K
authors.initials: SK
authors.lastname: Dower
mesh_terms: D000818:Animals; D002460:Cell Line; D005796:Genes / Q000502:physiology*; D017930:Genes, Reporter; D023281:Genomics / Q000379:methods; D006801:Humans; D016296:Mutagenesis; D025941:Protein Interaction Mapping; D011506:Proteins / Q000235:genetics / Q000502:physiology*; D040901:Proteomics / Q000379:methods; D034622:RNA Interference; D015398:Signal Transduction
chemical_list: D011506:Proteins