Tuning the setup of sputter-coated multilayers in nanocluster-based signal-enhancing biochips for optimal performance in protein and DNA assays.
In biochip development two issues are critical: stable and specific immobilization of the ligand and achievement of high signal-to-background ratio. In this work we have addressed these issues for the development of biochips, produced by sputtering multilayers of thin metal films, metal oxides, and metal nitrides (tens to hundreds of nanometers thick) onto glass wafers. Optimized surfaces have shown good results in genomic and proteomic experiments with biochips based on surface-enhanced fluorescence and absorption techniques.
Matyushin
Viatcheslav
V
Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.
Gandhum
Arminder
A
Stich
Norbert
N
Schalkhammer
Thomas
T
Hagen
Wilfred R
WR
Mayer
Christian
C
D000327:Adsorption; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry*; D003460:Crystallization / Q000379:methods*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005740:Gases; D006358:Hot Temperature; D008422:Materials Testing; D008567:Membranes, Artificial; D008670:Metals / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D005740:Gases; D008567:Membranes, Artificial; D008670:Metals