Molecular cytogentic profiles of hepatitis C infection in patients at Sharkia Governorate, Egypt.
It has become apparent that hepatitis C virus (HCV) infection is a major risk factor for the development of hepatocellular carcinoma (HCC) worldwide. The precise mechanism by which HCV causes HCC is not known. Unlike the hepatitis B virus (HBV), HCV is not a DNA virus and does not become integrated within the genome of hepatocytes. It is more likely that HCC occurs against a background of inflammation and regeneration, associated with liver injury due to chronic hepatitis. In this study, 40 of paraffin blocks liver tissues from HCV-PCR positive patients (HBV seronegative) were examined using DNA image cytometry to evaluate its role in diagnosing HCC associated with HCV infection. Fluorescent in situ hybridization (FISH) technique using LSIZNF 217 chromosome 20q 13.2 probe was applied as well. The results showed high percentage of S-phase fraction in cases of G2S2 and G3S3 with DNA diploidy. Only two cases of G3S3 showed DNA aneuploidy with severe amplification of chromosome 20q 13.2. Consequently, DNA imaging cytometry is a good approach in differentiating dysplasia from well-differentiated HCC on top of HCV infection. In conclusion HCV has an acquired role in development of HCC through amplification of the aggressive tumor behavior oncogene LSIZNF 217 at chromosome 20q 13.2.
el-Sobky
Ezzat
E
Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Mangoud
Amal M
AM
Mahrous
Seham
S
Eissa
Mostafa H
MH
Sabee
Essam I
EI
Ibrahem
Ibrahem A
IA
Ismail
Alaa
A
Morsy
Tosson A
TA
Nor Edin
Essam
E
el-Naggar
Ismail
I
Mostafa
Yousry
Y
Abuel-Magd
Yousry
Y
Afefy
Afefy F
AF
el-Shorbagy
Eman
E
el-Sadawy
Mahmoud
M
Ragab
Hosnia
H
el-Far
Ahmed
A
Hassan
Mostafa I
MI
Lakouz
Khalid
K
Abdel-Aziz
Khalid
K
Saber
Mahmoud
M
el-Hady
Gaber
G
D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis; D004534:Egypt; D005260:Female; D005434:Flow Cytometry; D016679:Genome, Viral; D016174:Hepacivirus / Q000235:genetics; D019698:Hepatitis C, Chronic / Q000150:complications / Q000235:genetics* / Q000473:pathology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008099:Liver / Q000473:pathology / Q000821:virology; D008113:Liver Neoplasms / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics
D004273:DNA, Neoplasm; D012367:RNA, Viral