Glycine cleavage system in neurogenic regions.
The glycine cleavage system (GCS) is the essential enzyme complex for degrading glycine and supplying 5,10-methylenetetrahydrofolate for DNA synthesis. Inherited deficiency of this system causes nonketotic hyperglycinemia, characterized by severe neurological symptoms and frequent association of brain malformations. Although high levels of glycine have been considered to cause the above-mentioned problems, the detailed pathogenesis of this disease is still unknown. Here we show that GCS is abundantly expressed in rat embryonic neural stem/progenitor cells in the neuroepithelium, and this expression is transmitted to the radial glia-astrocyte lineage, with prominence in postnatal neurogenic regions. These data indicate that GCS plays important roles in neurogenesis, and suggest that disturbance of neurogenesis induced by deficiency of GCS may be the main pathogenesis of nonketotic hyperglycinemia.
Ichinohe
Akiko
A
Department of Medical Genetics, Tohoku University School of Medicine, Sendai 980-8574, Japan.
Kure
Shigeo
S
Mikawa
Sumiko
S
Ueki
Takatoshi
T
Kojima
Kanako
K
Fujiwara
Kazuko
K
Iinuma
Kazuie
K
Matsubara
Yoichi
Y
Sato
Kohji
K
D000594:Amino Acid Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D027322:Amino Acid Transport System X-AG / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology / Q000502:physiology*; D001923:Brain Chemistry; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D004847:Epithelial Cells / Q000166:cytology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods; D008099:Liver / Q000201:enzymology; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism*; D009457:Neuroglia / Q000201:enzymology*; D009474:Neurons / Q000201:enzymology*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014166:Transferases / Q000235:genetics / Q000378:metabolism*
D027322:Amino Acid Transport System X-AG; D002352:Carrier Proteins; D009097:Multienzyme Complexes; D012333:RNA, Messenger; C064525:glycine cleavage system; D000594:Amino Acid Oxidoreductases; D014166:Transferases