title: Removal of hepatitis A virus from water by polyacrylonitrile-based ultrafiltration membranes.
abstract: Ultrafiltration-based polyacrylonitrile membranes (UF-membranes) were evaluated for their ability to retain tissue culture adapted indigenous hepatitis A virus from water. Amicon cell (dead-end ultrafiltration unit)-based experiment was set up and viral assay was carried out using rapid and sensitive reverse transcriptase polymerase chain reaction (RT-PCR). The RT-PCR results show retention of virus particles by the polyacrylonitrile membranes. A protocol for routine virological evaluation of membranes is described and the use of these membranes for water purification units and for virus concentration systems for field application is discussed.
authors.affiliation: Hepatitis Division, National Institute of Virology, 20-A Dr. Ambedkar road, Post Box No. 11, Pune 411001, India. suravaidya@yahoo.com
authors.forename: Sunil R
authors.initials: SR
authors.lastname: Vaidya
authors.forename: Ulhas K
authors.initials: UK
authors.lastname: Kharul
authors.forename: Shobha D
authors.initials: SD
authors.lastname: Chitambar
authors.forename: Santosh D
authors.initials: SD
authors.lastname: Wanjale
authors.forename: Yogesh S
authors.initials: YS
authors.lastname: Bhole
mesh_terms: D000180:Acrylic Resins; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D030041:Hepatitis A virus / Q000235:genetics / Q000302:isolation & purification*; D008567:Membranes, Artificial; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014462:Ultrafiltration / Q000379:methods; D014773:Virology / Q000379:methods; D014871:Water Microbiology; D014881:Water Supply
chemical_list: D000180:Acrylic Resins; D004279:DNA, Viral; D008567:Membranes, Artificial; C010504:polyacrylonitrile