Actinobacillus pleuropneumoniae metalloprotease: cloning and in vivo expression.
The complete amino acid and nucleotide sequence of a secreted metalloprotease produced by Actinobacillus pleuropneumoniae serotype 1 is reported. A clone showing proteolytic activity in cell-free culture media was selected from a genomic library of A. pleuropneumoniae serotype 1 in pUC 19. The sequence obtained contained an open reading frame encoding a protein with 869 amino acids. This protein was identified as a zinc neutral-metalloprotease belonging to the aminopeptidase family, with a predicted molecular weight of approximately 101 kDa. This sequence showed high homology with other predicted or sequenced aminopeptidases reported for different Gram-negative bacteria. Expression of the protease was observed in lung tissue from pigs that died of porcine pleuropneumonia suggesting a role in pathogenesis.
García González
Octavio
O
Carrera de Biología, Facultad de Estudios Superiores Iztacala, UNAM, Av. de los Barrios #1, Los Reyes Iztacala, Tlalnepantla, Estado de México 54090, Mexico.
García
Rosa M
RM
de la Garza
Mireya
M
Vaca
Sergio
S
Paniagua
Gloria Luz
GL
Mejía
Ricardo
R
Tenorio
Víctor R
VR
Negrete-Abascal
Erasmo
E
D000189:Actinobacillus Infections / Q000382:microbiology / Q000662:veterinary; D016977:Actinobacillus pleuropneumoniae / Q000201:enzymology* / Q000235:genetics* / Q000472:pathogenicity; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005798:Genes, Bacterial; D008168:Lung / Q000473:pathology; D045726:Metalloproteases / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D011001:Pleuropneumonia / Q000382:microbiology / Q000662:veterinary; D011994:Recombinant Proteins / Q000378:metabolism; D017385:Sequence Homology; D013552:Swine / Q000382:microbiology; D013553:Swine Diseases / Q000382:microbiology
D004269:DNA, Bacterial; D011994:Recombinant Proteins; D045726:Metalloproteases