Construction and use of PCR primers from a 65 kDa mannoprotein gene for identification of C. albicans.
A method for detection of Candida albicans in biological samples (blood, serum, urine) was developed by the use of polymerase chain reaction (PCR) amplification of a DNA fragment of a gene coding for a 65 kDa mannoprotein of C. albicans (CaMP65). The PCR amplifies a 220 bp fragments whose specificity for C. albicans was demonstrated by Southern blot with a non-radioactive probe, leading to the differentiation from all other yeast species or human and bacterial DNA. The sensitivity of this assay was 5-10 C. albicans cells per milliliter of biological sample.
Arancia
S
S
Department of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161 Rome, Italy. rlavalle@iss.it
Sandini
S
S
Cassone
A
A
De Bernardis
F
F
La Valle
R
R
D015139:Blotting, Southern; D002176:Candida albicans / Q000235:genetics* / Q000302:isolation & purification*; D002177:Candidiasis / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics*; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008970:Molecular Weight; D016533:Mycological Typing Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity
D017931:DNA Primers; D004271:DNA, Fungal; D008562:Membrane Glycoproteins; C025363:mannoproteins