Use of the omp50 gene for identification of Campylobacter species by PCR.
We studied the prevalence of the omp50 gene and the Omp50 protein in Campylobacter strains. Immunodetection assays and DNA-DNA hybridizations showed that most C. coli strains tested were negative and most C. jejuni and C. lari strains tested were positive. A PCR assay was developed, using the omp50 gene as a species-specific target. We propose a combination of a hippurate test and an omp50 assay to perform identification of Campylobacter species.
Dedieu
Luc
L
EA 2197 and IFR 48, Faculté de Médecine, Université de la Méditerranée, 13385 Marseille Cedex 5, France.
Pagès
Jean-Marie
JM
Bolla
Jean-Michel
JM
D000818:Animals; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D002167:Campylobacter / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017000:Campylobacter coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D044863:Campylobacter lari / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D018272:Porins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D004269:DNA, Bacterial; D018272:Porins