title: The antiviral effect of interferon-beta against SARS-coronavirus is not mediated by MxA protein.
abstract: Severe acute respiratory syndrome (SARS) is caused by a novel coronavirus termed SARS-CoV. No antiviral treatment has been established so far. Interferons are cytokines which induce the synthesis of several antivirally active proteins in the cell. In this study, we demonstrated that multiplication of SARS-CoV in cell culture can be strongly inhibited by pretreatment with interferon-beta. Interferon-alpha and interferon-gamma, by contrast, were less effective. The human MxA protein is one of the most prominent proteins induced by interferon-beta. Nevertheless, no interference with SARS-CoV replication was observed in Vero cells stably expressing MxA. Therefore, other interferon-induced proteins must be responsible for the strong inhibitory effect of interferon-beta against SARS-CoV.
authors.affiliation: Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Universität Freiburg, D-79008 Freiburg, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Spiegel
authors.forename: Andreas
authors.initials: A
authors.lastname: Pichlmair
authors.forename: Elke
authors.initials: E
authors.lastname: Mühlberger
authors.forename: Otto
authors.initials: O
authors.lastname: Haller
authors.forename: Friedemann
authors.initials: F
authors.lastname: Weber
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D016899:Interferon-beta / Q000494:pharmacology*; D064547:Myxovirus Resistance Proteins; D045473:Severe acute respiratory syndrome-related coronavirus / Q000187:drug effects* / Q000502:physiology; D045169:Severe Acute Respiratory Syndrome / Q000821:virology; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects
chemical_list: C579071:MX1 protein, human; D064547:Myxovirus Resistance Proteins; D016899:Interferon-beta; D019204:GTP-Binding Proteins