Effect of two nitrogenous diphenyl ether pesticides on mast cell activation.
We examined the effect of two nitrogenous diphenyl ether pesticides, nitrofen (NIP) and chlornitrofen (CNP), on mast cell activation. RBL-2H3 (rat basophilic leukemia) cells were exposed to NIP or CNP for 30 min to investigate their effect on degranulation, and for 3 h to investigate their effect on cytokine production and gene expression. NIP and CNP increased IgE receptor-mediated beta-hexosaminidase release, MCP-1 release, and TNF-alpha release in a dose-dependent manner. The increasing effect of CNP on their release was greater than that of NIP. In the gene expression experiment, 30 microg/ml CNP significantly upregulated Egr-1, MCP-1 and GADD45a gene expression. These results suggest that at higher concentrations (more than 30 microg/ml) the nitrogenous diphenyl ether pesticides had both a degranulation-enhancing effect and proinflammatory cytokine-production enhancing effect through the expression of some transcription factors in RBL-2H3 cells.
Teshima
Reiko
R
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences, Kamiyoga 1-18-1, Tokyo 158-8501, Japan. rteshima@nihs.go.jp
Nakamura
Ryosuke
R
Nakajima
Osamu
O
Hachisuka
Akiko
A
Sawada
Jun-Ichi
J
D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics; D015550:Cell Degranulation / Q000187:drug effects*; D045744:Cell Line, Tumor; D018932:Chemokine CCL2 / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D008407:Mast Cells / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D052066:N-Ethylmaleimide-Sensitive Proteins; D009687:Nuclear Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010575:Pesticides / Q000633:toxicity*; D010647:Phenyl Ethers / Q000633:toxicity*; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D015854:Up-Regulation; D033921:Vesicular Transport Proteins / Q000235:genetics; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism; D000097642:GADD45 Proteins
D018797:Cell Cycle Proteins; D018932:Chemokine CCL2; D052066:N-Ethylmaleimide-Sensitive Proteins; D009687:Nuclear Proteins; D010575:Pesticides; D010647:Phenyl Ethers; D014409:Tumor Necrosis Factor-alpha; D033921:Vesicular Transport Proteins; D001619:beta-N-Acetylhexosaminidases; C491766:Ccl2 protein, rat; C499073:Gadd45a protein, rat; D000097642:GADD45 Proteins; C010422:2,4,6-trichlorophenyl 4-nitrophenyl ether; C498737:Nsf protein, rat; C007350:nitrofen