Antiherpetic mode of action of (22S,23S)-3beta-bromo-5alpha,22,23-trihydroxystigmastan-6-one in vitro.
The replication of herpes simplex virus (HSV) type 1 in Vero cells is inhibited in the presence of (22S,23S)-3beta-bromo-5alpha,22,23-trihydroxystigmastan-6-one (6b), a synthetic brassinosteroid derivative. Attempts to disclose the mode of action of 6b indicate that a late step of virus multiplication is affected. In the presence of 6b, HSV late protein synthesis was severely diminished and this inhibitory effect of 6b on HSV antigen expression was confirmed by immunofluorescence assays.
Wachsman
Mónica B
MB
Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, Piso 4, 1428 Buenos Aires, Argentina. wachsman@qb.fcen.uba.ar
Castilla
Viviana
V
Talarico
Laura B
LB
Ramirez
Javier A
JA
Galagovsky
Lydia R
LR
Coto
Celia E
CE
D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis / Q000187:drug effects; D000998:Antiviral Agents / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D002778:Cholestanones / Q000494:pharmacology*; D019084:Fluorescent Antibody Technique, Indirect; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000254:growth & development*; D014158:Transcription, Genetic / Q000187:drug effects; D014709:Vero Cells; D014764:Viral Proteins; D014779:Virus Replication / Q000187:drug effects*
C493970:3-bromo-5,22,23-trihydroxystigmastan-6-one; D000956:Antigens, Viral; D000998:Antiviral Agents; D002778:Cholestanones; D014764:Viral Proteins