Spatial and temporal expression of ligands for CXCR3 and CXCR4 in human endometrium.
In this study, we investigated the expression of ligands for CXCR3 (Mig, IP-10, and I-TAC) and CXCR4 (SDF-1) in the human endometrium throughout the menstrual cycle. By immunohistochemistry, immunostaining for Mig and IP-10 was found in the surface epithelia, glandular epithelia, and stroma with some menstrual cycle-dependent fluctuation. By contrast, immunostaining for I-TAC or SDF-1 was not detected. ELISA demonstrated that the concentrations of Mig and IP-10 were higher in the secretory phase than in the proliferative phase, but I-TAC and SDF-1alpha was detected in only a few samples. Endometrial Mig and IP-10 concentrations strongly correlated with the number of endometrial natural killer cells. Progesterone significantly induced Mig secretion and tended to induce IP-10 secretion from the cultured endometrial stromal cells, whereas 17beta-estradiol had no significant effect. Neither I-TAC nor SDF-1alpha was detected in the supernatant of cultured endometrial stromal cells in the presence or absence of 17beta-estradiol or progesterone. The results suggest that Mig and IP-10 may be involved in the recruitment of natural killer cells or other phenomena in the human endometrium.
Kitaya
Kotaro
K
Departments of Obstetrics and Gynecology, Koto Prefectural University of Medicine, Kyoto 602-8566, Japan. kitaya@koto.kpu-m.ac.jp
Nakayama
Takeshi
T
Daikoku
Nobue
N
Fushiki
Shinji
S
Honjo
Hideo
H
D000328:Adult; D002478:Cells, Cultured; D054357:Chemokine CXCL10; D054371:Chemokine CXCL11; D054377:Chemokine CXCL12; D054370:Chemokine CXCL9; D019743:Chemokines, CXC / Q000378:metabolism*; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D008024:Ligands; D054367:Receptors, CXCR3; D019718:Receptors, CXCR4 / Q000378:metabolism; D019707:Receptors, Chemokine / Q000378:metabolism; D013194:Staining and Labeling; D013997:Time Factors; D014018:Tissue Distribution
C515202:CXCL11 protein, human; C515255:CXCL12 protein, human; C080810:CXCL9 protein, human; C515187:CXCR3 protein, human; D054357:Chemokine CXCL10; D054371:Chemokine CXCL11; D054377:Chemokine CXCL12; D054370:Chemokine CXCL9; D019743:Chemokines, CXC; D036341:Intercellular Signaling Peptides and Proteins; D008024:Ligands; D054367:Receptors, CXCR3; D019718:Receptors, CXCR4; D019707:Receptors, Chemokine