Inhibitory effects of prior low-dose X-ray irradiation on carbon tetrachloride-induced hepatopathy in acatalasemic mice.
The catalase activities in blood and organs of the acatalasemic (C3H/AnLCs(b)Cs(b)) mouse of C3H strain are lower than those of the normal (C3H/AnLCs (a)Cs(a)) mouse. We examined the effects of prior low-dose (0.5 Gy) X-ray irradiation, which reduced the oxidative damage under carbon tetrachloride-induced hepatopathy in the acatalasemic or normal mice. The acatalasemic mice showed a significantly lower catalase activity and a significantly higher glutathione peroxidase activity compared with those in the normal mice. Moreover, low-dose irradiation increased the catalase activity in the acatalasemic mouse liver to a level similar to that of the normal mouse liver. Pathological examinations and analyses of blood glutamic oxaloacetic and glutamic pyruvic transaminase activity and lipid peroxide levels showed that carbon tetrachloride induced hepatopathy was inhibited by low-dose irradiation. These findings may indicate that the free radical reaction induced by the lack of catalase and the administration of carbon tetrachloride is more properly neutralized by high glutathione peroxidase activity and low-dose irradiation in the acatalasemic mouse liver.
Yamaoka
Kiyonori
K
Chair of Medical Radioscience, Okayama University Medical School, Japan. yamaoka@md.okayama-u.ac.jp
Kataoka
Takahiro
T
Nomura
Takaharu
T
Taguchi
Takehito
T
Wang
Da-Hong
DH
Mori
Shuji
S
Hanamoto
Katsumi
K
Kira
Shohei
S
D020642:Acatalasia / Q000097:blood* / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D000975:Antioxidants / Q000032:analysis; D002252:Carbon Tetrachloride Poisoning; D002374:Catalase / Q000378:metabolism; D004351:Drug Resistance / Q000528:radiation effects*; D005978:Glutathione / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D008106:Liver Cirrhosis, Experimental / Q000097:blood* / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008809:Mice, Inbred C3H; D013482:Superoxide Dismutase / Q000378:metabolism; D000637:Transaminases / Q000097:blood; D014965:X-Rays
D000975:Antioxidants; D008054:Lipid Peroxides; D002374:Catalase; D013482:Superoxide Dismutase; D000637:Transaminases; D005978:Glutathione