[The study of yi-shen-huo-xue fang's effects on expression of GMP-140 and cleaning out the oxygenic free radicle on rabbits blood stasis model].
OBJECTIVE
To evaluate the effects of Yi-Shen-Huo-Xue Fang on expression of GMP-140 and cleaning out the oxygenic free radicle on rabbits blood stasis model.


METHODS
Thirty rabbits were divided randomly into five groups as the normal group, model group, large dose of "Yi-Shen-Huo-Xue Fang" group, small dose of "Yi-Shen-Huo-Xue Fang" group and "Xue-Shuan-Xin-Mai-Ning" group. After being treated respectively, granule membrane protein 140(GMP-140), erythrocyte sueroxide dismutase (E-SOD), erythrocyte lipid peroxide(E-LPO), plasma lipid peroxide(P-LPO) were checked up.


RESULTS
The GMP-140, E-SOD, E-LPO, P-LPO in normal control were compared with those in model groups, With the difference(P < 0.01), model control group was compared with large dose group and small dose group (P < 0.01), with "Xue-Shuan-Xin-Mai-Ning" group(P < 0.05), large dose group was compared with "Xue-Shuan-Xin-Mai-Ning" group(P < 0.05), and large dose group were compared with small dose group (P > 0.05).


CONCLUSIONS
The model was made successfully. Large dose group, small dose group and "xue-shuan-xin-mai-ning" group can inhibit expression of GMP-140, enhence SOD activity and decrease LPO content on blood stasis rabbit model. Large dose group and small dose group have stronger effect than "xue-shuan-xin-mai-ning" group.
Zhan
Xiao-ping
XP
Second Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, Zhejiang, China.
Fang
Qing
Q
Lou
Jian-guo
JG
Xu
Xi-hong
XH
Jin
Xiao-ying
XY
Qin
Guang-ming
GM
Bao
Jun
J
D000818:Animals; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D008516:Medicine, Chinese Traditional; D019007:P-Selectin / Q000096:biosynthesis*; D010946:Plants, Medicinal / Q000737:chemistry; D011817:Rabbits; D011897:Random Allocation; D013482:Superoxide Dismutase / Q000097:blood
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D008054:Lipid Peroxides; D019007:P-Selectin; D013482:Superoxide Dismutase