Mapping of the insulin-like growth factor II binding site of the Type I insulin-like growth factor receptor by alanine scanning mutagenesis.
The Type I insulin-like growth factor receptor is a physiological receptor for insulin-like growth factor II (IGF-II). To characterize the molecular basis of the receptor's ligand binding properties, we have examined the effects of alanine mutations of residues in the ligand binding site of the receptor on its affinity for IGF-II. The functional epitope for IGF-II comprises residues in the N-terminal L1 domain and residues at the C-terminus of the alpha subunit. Cysteine rich domain residues do not appear to be critical for IGF-II binding.
Sørensen
Heidi
H
Receptor Biology Laboratory, Hagedorn Research Institute, 2820 Gentofte, Denmark.
Whittaker
Linda
L
Hinrichsen
Jane
J
Groth
Andreas
A
Whittaker
Jonathan
J
D000409:Alanine / Q000737:chemistry / Q000235:genetics*; D001665:Binding Sites; D002460:Cell Line; D003545:Cysteine / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D000939:Epitopes / Q000737:chemistry; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000737:chemistry* / Q000502:physiology; D007335:Insulin-Like Growth Factor II / Q000737:chemistry / Q000235:genetics*; D007700:Kinetics; D008024:Ligands; D008958:Models, Molecular; D016296:Mutagenesis; D009154:Mutation; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary
D018076:DNA, Complementary; D000939:Epitopes; D008024:Ligands; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D003545:Cysteine; D000409:Alanine