title: traT and CNF2 genes of Escherichia coli isolated from milk of healthy cows and sheep.
abstract: The objectives of the present study were to isolate Escherichia coli from milk of apparently healthy cows and sheep and to investigate the presence of traT and cytotoxic necrotising factor-2 (CNF2) virulence genes by multiplex polymerase chain reaction (PCR). Milk samples collected from a total of 1028 apparently healthy ruminants (737 cows and 291 sheep) in eastern Turkey were streaked onto 5% sheep-blood agar. E. coli was isolated and identified by biochemical tests in 5.9% (61/1028) of milk samples. Correct amplification with the molecular length of 232 bp was obtained from all E. coli isolates by the species-specific PCR. The isolation rates of the agent were calculated to be 7.6% (56/737) in cows and 1.7% (5/291) in sheep. The difference between these proportions was statistically significant (p < 0.001). Multiplex PCR showed that traT and CNF2 genes were present in 62.3% and 6.6% of all isolates, respectively. Both genes were present in 16.4% of the isolates, with only 14.7% having neither gene.
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, University of Firat, 23119 Elazig, Turkey.
authors.forename: M N
authors.initials: MN
authors.lastname: Açik
authors.forename: N E
authors.initials: NE
authors.lastname: Yurdakul
authors.forename: L
authors.initials: L
authors.lastname: Cakici
authors.forename: N
authors.initials: N
authors.lastname: Onat
authors.forename: O
authors.initials: O
authors.lastname: Dogan
authors.forename: B
authors.initials: B
authors.lastname: Cetinkaya
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D015373:Bacterial Typing Techniques / Q000662:veterinary; D002417:Cattle; D003603:Cytotoxins / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics; D008892:Milk / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012756:Sheep
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; D003603:Cytotoxins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C036297:TraT protein, E coli; C086995:cytotoxic necrotizing factor type 2