title: The role of allogenic fibroblasts in an acute wound healing model.
abstract: Skin is the first tissue-engineered organ to have been successfully developed in the laboratory, and it has been clinically available for use as epidermal sheets for some time. As refinements in this field of tissue engineering continue, several key issues give cause for concern. One issue is the need to form a more complete dermal analogue before grafting. To this end, fibroblasts may be used in vitro to deposit extracellular matrix components within a basic scaffold, laying down those molecules not endogenous to the material and thereby improving the quality of the skin replacement. Many studies have shown the benefits of in vitro seeding with fibroblasts, but there has been some debate regarding the longevity of such cells after allotransplantation into an immunocompetent host. In this study, the authors set out to determine the longevity of transplanted cells in an immunocompetent porcine model. A total of 24 wounds were made on four female animals, 12 of which were covered with acellular hyaluronic acid dermal matrices, and the remainder of which were covered with matrices seeded with allogenic (male) fibroblasts. After a week in vivo, the wounds were grafted with either split-thickness skin grafts or cultured epithelial autograft. Biopsy specimens were obtained from wounds at varying time intervals and assessed using genetic analysis to determine the survival of allotransplanted cells. No cells were detectable by polymerase chain reaction analysis (sensitivity, 1:100,000) after 7 days in vivo. Subsequent histologic examination demonstrated little difference in wound morphology. The authors conclude that allogenic fibroblasts do not survive transplantation in a porcine wound model.
authors.affiliation: Department of Plastic Surgery, Wythenshawe Hospital, Queen Mary, University of London, Barts & London Medical School, 2 Newark Street, London, United Kingdom.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Price
authors.forename: Victoria
authors.initials: V
authors.lastname: Das-Gupta
authors.forename: Paul A
authors.initials: PA
authors.lastname: Harris
authors.forename: Irene M
authors.initials: IM
authors.lastname: Leigh
authors.forename: Harshad A
authors.initials: HA
authors.lastname: Navsaria
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D062109:Dermatologic Surgical Procedures; D020405:Dermis / Q000378:metabolism; D004848:Epithelium / Q000637:transplantation; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000637:transplantation*; D006085:Graft Survival; D015603:Keratinocytes / Q000637:transplantation; D008297:Male; D016133:Polymerase Chain Reaction; D012867:Skin / Q000293:injuries* / Q000473:pathology; D016038:Skin Transplantation; D017768:Skin, Artificial; D013552:Swine; D023822:Tissue Engineering; D014184:Transplantation, Homologous; D014945:Wound Healing
chemical_list: D004247:DNA