title: Dogs vaccinated with common Lyme disease vaccines do not respond to IR6, the conserved immunodominant region of the VlsE surface protein of Borrelia burgdorferi.
abstract: A 25-amino-acid synthetic peptide (C(6) peptide) derived from an immunodominant conserved region (designated IR(6)) of the VlsE protein of Borrelia burgdorferi has been identified and used to construct immunoenzyme-based diagnostic procedures. These procedures have excellent sensitivity and specificity. Previous reports have demonstrated the usefulness of the C(6) peptide as an antigen for the serodiagnosis of human and canine Lyme disease. Results indicated that assays based on the C(6) peptide were nonreactive to sera from vaccinated nonexposed animals. The purpose of the present study was to confirm these results in a controlled trial by testing sera from experimentally vaccinated dogs known to be uninfected. Nine specific-pathogen-free beagles were assigned to one of three vaccine groups, each containing three dogs. Each group received one of three commercial Lyme vaccines: RECOMBITEK Lyme (Merial), LymeVax (Fort Dodge Animal Health), and Galaxy Lyme (Schering-Plough Animal Health). Each animal was administered a total of five doses of vaccine over a period of 39 weeks. Serum samples were collected prior to vaccination and then on a weekly basis from weeks 3 to 18 and from weeks 33 to 43. Selected samples were tested by the immunofluorescence assay (IFA) and the Western blot (WB) assay using whole-cell B. burgdorferi antigen extracts, and the results were compared to those obtained with two immunoenzyme-based procedures formatted by using the C(6) peptide. Serum specimens from all animals were reactive to the IFA and WB assay at week 5 and became highly reactive following the administration of multiple doses of vaccine. All serum specimens were uniformly nonreactive in the C(6) peptide immunoenzyme procedures at all time points throughout the study.
authors.affiliation: Department of Research and Development, IDEXX Laboratories, One IDEXX Dr., Westbrook, ME 04092, USA. tom-oconnor@idexx.com
authors.forename: Thomas P
authors.initials: TP
authors.lastname: O'Connor
authors.forename: Kathy J
authors.initials: KJ
authors.lastname: Esty
authors.forename: Jancy L
authors.initials: JL
authors.lastname: Hanscom
authors.forename: Paulette
authors.initials: P
authors.lastname: Shields
authors.forename: Mario T
authors.initials: MT
authors.lastname: Philipp
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D001428:Bacterial Vaccines; D015153:Blotting, Western; D025065:Borrelia burgdorferi / Q000276:immunology*; D004283:Dog Diseases / Q000382:microbiology / Q000517:prevention & control; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007118:Immunoassay; D016056:Immunodominant Epitopes / Q000276:immunology*; D008074:Lipoproteins / Q000276:immunology*; D008193:Lyme Disease / Q000517:prevention & control / Q000662:veterinary; D022123:Lyme Disease Vaccines / Q000008:administration & dosage / Q000276:immunology*; D008297:Male; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D013047:Specific Pathogen-Free Organisms; D014611:Vaccination
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D016056:Immunodominant Epitopes; D008074:Lipoproteins; D022123:Lyme Disease Vaccines; C072045:OspA protein; D010455:Peptides; C105546:VlsE protein, Borrelia burgdorferi; C077030:OspB protein, Borrelia burgdorferi