[Analysis of expression of a series of lymphoma-specific genes in human fibroblasts immortalized by SV40 virus].
Subtraction hybridization was earlier used to obtain cDNA clones corresponding to human genes upregulated in HIV-associated centroblast lymphoma (CL) as compared with HIV-associated immunoblast lymphoma (IL). With inverse subtraction hybridization, clones were isolated that correspond to genes upregulated in IL compared with CL. In addition to cDNAs characterized earlier, the resulting clones contained several (seven CL-specific and three IL-specific) sequences with unknown functions. To identify the lymphoma-specific genes that are overexpressed in early carcinogenesis, Northern blotting was used to assess the level of gene transcription in two human fibroblast lines and in their derivatives immortalized with either a temperature-sensitive mutant of SV-40 or with pSV3neo carrying the SV-40 A gene, considering the latter as a model of early cell malignant transformation. Increased expression in at least one immortalized line compared with normal fibroblasts was observed for set, a-myb, ND1, ND2, ND4 (NADH dehydrogenase subunits 1, 2, and 4), COX2, COX3 (cytochrome-c-oxidase subunits 2 and 3), KIAA0129, and the gene corresponding to cDNA hss2-1-7-10. High expression of these genes was assumed to be associated not only with lymphomogenesis, but also with early transformation (immortalization) of other, nonlymphoid cells. Expression of the calpain gene and the gene corresponding to cDNA hss2-2-9-5 proved to be lower in immortalized than in normal fibroblasts. This was considered indicative of an alternative mechanism of fibroblast transformation or of different processes regulating the expression of these genes in early and late carcinogenesis.
Nenasheva
V V
VV
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182 Russia. val-nenasheva@mail.ru
Nikolaev
A I
AI
Dubovaia
V I
VI
Inozemtseva
L S
LS
Manuilova
E S
ES
Martynenko
A V
AV
Tarantul
V Z
VZ
D001483:Base Sequence; D002461:Cell Line, Transformed; D017931:DNA Primers; D018076:DNA, Complementary; D005347:Fibroblasts / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D016483:Lymphoma, AIDS-Related / Q000235:genetics*; D013539:Simian virus 40 / Q000502:physiology
D017931:DNA Primers; D018076:DNA, Complementary