Inhibition of Cav3.1 channel by silver ions.
We have investigated the effects of AgCl and AgNO3 on the Cav3.1 calcium channels stably expressed in the HEK 293 cells. Ca2+ was used as a charge carrier. Both forms of Ag+ blocked the Cav3.1 channel and negatively shifted the I-V relations in a concentration-dependent manner. The inhibition of current amplitude by AgCl was voltage-dependent and increased with increasing amplitude of the depolarizing pulse. Furthermore, AgCl but not AgNO3 accelerated the kinetics of current activation. No effect on current inactivation or steady-state inactivation of the channel was observed for AgCl or AgNO3.
Gromová
P
P
Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Bratislava, Slovakia.
Kurejová
M
M
Lacinová
L
L
D020746:Calcium Channels, L-Type / Q000187:drug effects* / Q000502:physiology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology; D007668:Kidney / Q000187:drug effects* / Q000196:embryology / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects* / Q000502:physiology*; D012834:Silver / Q000494:pharmacology*; D018030:Silver Compounds / Q000494:pharmacology; D012835:Silver Nitrate / Q000494:pharmacology
C485995:Cacna1d protein, mouse; D020746:Calcium Channels, L-Type; D018030:Silver Compounds; D012834:Silver; D012835:Silver Nitrate; C037548:silver chloride