title: Bactericidal effects of acidic electrolyzed water on the dental unit waterline.
abstract: Many studies have been conducted in the United States regarding the microbial contamination of dental unit waterline, but not in Japan. Recently, acidic electrolyzed water has been used in the medical and dental fields. In this study, we investigated the bactericidal effects of the temporary inflow of acidic electrolyzed water on microbial contamination of the dental unit waterline. First, in order to observe the daily bacterial contamination of the dental unit waterline, water samples were collected at the end of handpieces and three-way syringes before the inflow of acidic electrolyzed water. They were cultured to detect viable bacteria. Later, the inflow of acidic electrolyzed water was conducted through the piping box of the dental unit. Before starting operation on next day, water samples were collected and cultured, as described above. The mean viable bacteria count was 910 -/+ 190 CFU/ml at the end of handpieces, and 521 -/+ 116 CFU/ml at the end of three-way syringes before the inflow of acidic electrolyzed water. However, bacteria were detected in only small numbers at the end of handpieces and three-way syringes on the next day. These results indicated that acidic electrolyzed water could be applied as an appropriate measure against bacterial contamination of the dental unit waterline.
authors.affiliation: Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University, Hiroshima 734-8553, Japan. acho@hiroshima-u.ac.jp
authors.forename: Shinya
authors.initials: S
authors.lastname: Kohno
authors.forename: Toshitsugu
authors.initials: T
authors.lastname: Kawata
authors.forename: Masato
authors.initials: M
authors.lastname: Kaku
authors.forename: Tadashi
authors.initials: T
authors.lastname: Fuita
authors.forename: Keisuke
authors.initials: K
authors.lastname: Tsutsui
authors.forename: Junji
authors.initials: J
authors.lastname: Ohtani
authors.forename: Kaoru
authors.initials: K
authors.lastname: Tenjo
authors.forename: Masahide
authors.initials: M
authors.lastname: Motokawa
authors.forename: Yuiko
authors.initials: Y
authors.lastname: Tohma
authors.forename: Mao
authors.initials: M
authors.lastname: Shigekawa
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kamata
authors.forename: Kazuo
authors.initials: K
authors.lastname: Tanne
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D003748:Dental Equipment / Q000382:microbiology*; D004203:Disinfection / Q000379:methods*; D004572:Electrolysis; D004866:Equipment Contamination / Q000517:prevention & control*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D014871:Water Microbiology