FOS expression in orexin neurons following muscimol perfusion of preoptic area.
Unilateral microdialysis-perfusion of the preoptic area with 50 microM muscimol decreased the sleep period of rats to less than 3% of the baseline value over a 90 min period before death (p = 0.018 by Wilcoxon signed-rank test). These rats showed the expression of FOS in 36% of the orexin neurons located in the perifornical/lateral hypothalamic areas on the side ipsilateral to the perfusion site, but in only 3% of the orexin neurons on the side contralateral to it (p = 0.018 by Wilcoxon signed-rank test). These results suggest that subpopulations of the preoptic neurons give an inhibitory tone to the activities of the orexin neurons in the perifornical/lateral hypothalamic areas.
Satoh
Shinsuke
S
Department of Neuropsychiatry, Osaka Medical College, Takatsuki, Osaka 569-8686, Japan. knssat@yahoo.com
Matsumura
Hitoshi
H
Fujioka
Atsuko
A
Nakajima
Tomoko
T
Kanbayashi
Takashi
T
Nishino
Seiji
S
Shigeyoshi
Yasufumi
Y
Yoneda
Hiroshi
H
D000818:Animals; D001143:Arousal / Q000187:drug effects / Q000502:physiology; D002352:Carrier Proteins / Q000032:analysis / Q000096:biosynthesis*; D005786:Gene Expression Regulation / Q000502:physiology; D007276:Injections, Intraventricular; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D009118:Muscimol / Q000008:administration & dosage*; D009474:Neurons / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D009479:Neuropeptides / Q000032:analysis / Q000096:biosynthesis*; D000068797:Orexins; D010477:Perfusion / Q000379:methods; D011301:Preoptic Area / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012890:Sleep / Q000187:drug effects / Q000502:physiology
D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009479:Neuropeptides; D000068797:Orexins; D016760:Proto-Oncogene Proteins c-fos; D009118:Muscimol