Reversible immobilization of engineered molecules by Ni-NTA chelators.
Electrochemical synthesis of nickel-nitrilotriacetic acid (Ni-NTA) chelators, for subsequent immobilization of (His)(6)-tagged proteins (Photosystem II (PSII) as model molecule), on Au or Au-graphite electrodes is compared to chemical synthesis. Results show: (i) higher Ni-NTA surface density, (ii) shorter treatment time (1-12 min vs. 16 h normally needed for self-assembled monolayer (SAM)), (iii) possibility of addressing the chelator to only one Au electrode, in a sensor micro-array.
Maly
J
J
Department of Biology, University of J.E. Purkyne, Usti nad Labem, Czech Republic.
Di Meo
C
C
De Francesco
M
M
Masci
A
A
Masojidek
J
J
Sugiura
M
M
Volpe
A
A
Pilloton
R
R
D000327:Adsorption; D015374:Biosensing Techniques / Q000379:methods*; D002614:Chelating Agents / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D008027:Light; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives* / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D045332:Photosystem II Protein Complex / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics / Q000528:radiation effects; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000737:chemistry / Q000528:radiation effects
D002614:Chelating Agents; D020099:Coated Materials, Biocompatible; D009942:Organometallic Compounds; D045332:Photosystem II Protein Complex; D011994:Recombinant Proteins; C088321:nickel nitrilotriacetic acid; D009571:Nitrilotriacetic Acid