title: Validated 5' nuclease PCR assay for rapid identification of the genus Brucella.
abstract: A real-time, genus-specific 5' nuclease PCR assay for amplification of a 322-bp fragment of the per gene was developed for rapid (<2 h) identification of Brucella spp. from agar plates. The assay, including an internal amplification control (116 bp), identified Brucella strains (n = 23) and did not detect non-Brucella strains (n = 174), indicating its usefulness, particularly for laboratories with stringent quality assurance programs.
authors.affiliation: University of Turku, Turku, Finland.
authors.forename: T
authors.initials: T
authors.lastname: Bogdanovich
authors.forename: M
authors.initials: M
authors.lastname: Skurnik
authors.forename: P S
authors.initials: PS
authors.lastname: Lübeck
authors.forename: P
authors.initials: P
authors.lastname: Ahrens
authors.forename: J
authors.initials: J
authors.lastname: Hoorfar
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D001483:Base Sequence; D002002:Brucella / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification*; D002006:Brucellosis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary; D002238:Carbohydrate Epimerases / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D000637:Transaminases / Q000235:genetics
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D000637:Transaminases; D002238:Carbohydrate Epimerases; C061272:perosamine synthetase