Characterisation of autolytic enzymes in Lactobacillus pentosus.
OBJECTIVE
To characterize autolysis and autolytic system of the lactic acid bacterium Lactobacillus pentosus.


RESULTS
Autolysis of nine Lact. pentosus strains was evaluated in buffer solution. Their peptidoglycan hydrolase profiles were examined by renaturing SDS-PAGE and revealed two major activity bands at 58 and 112 kDa. Specificity analysis indicated the presence of at least two different types of peptidoglycan hydrolase activities in Lact. pentosus 1091.


CONCLUSIONS
Autolysis of Lact. pentosus was shown to be strain dependent and involvement of at least two different autolysins was evidenced.


CONCLUSIONS
The autolytic system of Lact. pentosus was characterized for the first time and the data obtained could be used in the selection of strains of technological interest.
Cibik
R
R
Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, University of Uludag, Bursa, Turkey.
Chapot-Chartier
M-P
MP
D001433:Bacteriolysis; D002241:Carbohydrates / Q000032:analysis; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D005516:Food Microbiology; D007778:Lactobacillus / Q000201:enzymology*; D008970:Molecular Weight; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D013379:Substrate Specificity
D002241:Carbohydrates; D009238:N-Acetylmuramoyl-L-alanine Amidase