Enantioseparation of four cis and trans diastereomers of 2',3'-didehydro-2',3'-dideoxythymidine analogs, by high-performance liquid chromatography and capillary electrophoresis.
Compounds 1-4 are the four stereoisomers of a synthetic new potential antiviral agent (d4T analog) containing two chiral centers and a base (uracil). Both high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE) techniques were used to separate and quantify enantiomers with high resolution. The determination of enantiomeric purity of the compounds was developed using both amylose chiral stationary phase by HPLC and anionic cyclodextrins (highly S-CD) as chiral selectors in CE. The HPLC method was found to be superior in sensitivity to the CE method.
Lipka
E
E
Laboratoire de Chimie Analytique, Faculté des Sciences Pharmaceutiques et Biologiques, Université de Lille II, BP 83-3, rue du Pr. Laguesse, 59006 Lille Cedex, France.
Selouane
A
A
Postel
D
D
Len
C
C
Vaccher
M P
MP
Bonte
J P
JP
Vaccher
C
C
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D015203:Reproducibility of Results; D018894:Reverse Transcriptase Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D018119:Stavudine / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism
D018894:Reverse Transcriptase Inhibitors; D018119:Stavudine