[Association between a genomic polymorphism within the CD14 locus and severe sepsis susceptibility as well as prognosis in patients after extensive burns].
OBJECTIVE
To investigate the relation of a lipopolysaccharide receptor CD14C-159T gene polymorphism to severe sepsis susceptibility and prognosis in patients with extensive burns.


METHODS
The study group consisted of 118 normal controls and 16 patients with burns covering more than 60 percent total body surface area. Typing of each patient for the CD14C-159T gene polymorphism was performed by analyzing restriction fragments of a Hae III-digested DNA fragment obtained using polymerase chain reaction (PCR-RFLP). Genotypes were then related to the susceptibility and mortality rate of severe sepsis.


RESULTS
The overall allele frequency (C 43.7 percent, T 56.3 percent) and genotype distribution (C homozygous 12.5 percent, C/T 62.5 percent, T homozygous 25.0 percent) were in agreement with the distribution in healthy volunteers. Genotype distribution in patients with severe sepsis was different from that in patients with an uncomplicated clinical course. Development of severe sepsis was increased in patients homozygous for the allele T (71.4 percent) than that of the non- sepsis patients (44.4 percent).


CONCLUSIONS
The single base pair polymorphism at position-159 in the CD14 gene promoter might influence the development of severe sepsis in patients with extensive burns.
Lin
Jing
J
Burns Institute, 304th Hospital of PLA, Beijing 100037, China.
Yao
Yong-ming
YM
Huang
Zhi-hong
ZH
Hou
Xiao-xia
XX
Zhu
Jing-min
JM
Chai
Jia-ke
JK
D000328:Adult; D002056:Burns / Q000150:complications*; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011379:Prognosis; D018805:Sepsis / Q000209:etiology / Q000235:genetics* / Q000401:mortality; D015996:Survival Rate
D005819:Genetic Markers; D018950:Lipopolysaccharide Receptors