title: Oxidative DNA damage in placentas from normal and pre-eclamptic pregnancies.
abstract: Placental oxidative stress was suggested to play a role in the pathogenesis of pre-eclampsia (PE). In this study, levels of 8-hydroxy-2'-deoxyguanosine (8-OH-dG), a well-established marker of oxidative DNA damage, were analysed in placental cellular DNA from normal (group NP) and pre-eclamptic (group PE) pregnancies as well as from PE pregnancies complicated by intrauterine growth restriction (group PE-IUGR). Placental samples obtained immediately after delivery were frozen at -80 degrees C until analysis. Cellular DNA was isolated, hydrolysed and analysed using high-performance liquid chromatography. Native nucleosides were monitored at 254 nm and 8-OH-dG using electrochemical detection. Concentrations of 8-OH-dG were expressed as micro mol/mol 2'-deoxyguanosine. In group NP, mean concentration of 8-OH-dG reached 179.97+/-80.58 (+/-SEM; micro mol/mol dG). 8-OH-dG levels were higher in group PE (273.44+/-110.14 micro mol/mol), but the difference was not significant in comparison with group NP. Highest concentrations of 8-OH-dG were found in group PE-IUGR (428.97+/-141.40 micro mol/mol), with levels significantly higher than in group NP, but not group PE. The results indicate a positive correlation between the severity of PE and the degree of oxidative stress and corroborate previous studies suggesting reactive oxygen species to be involved in the pathophysiology of PE.
authors.affiliation: Department of Obstetrics and Gynaecology, Medical University School, Lublin, Poland.
authors.forename: Henryk
authors.initials: H
authors.lastname: Wiktor
authors.forename: Marta
authors.initials: M
authors.lastname: Kankofer
authors.forename: Ivo
authors.initials: I
authors.lastname: Schmerold
authors.forename: Agnes
authors.initials: A
authors.lastname: Dadak
authors.forename: Maciej
authors.initials: M
authors.lastname: Lopucki
authors.forename: Hans
authors.initials: H
authors.lastname: Niedermüller
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D005260:Female; D005317:Fetal Growth Retardation / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007231:Infant, Newborn; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010920:Placenta / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D011225:Pre-Eclampsia / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine