A recessive mutation in the RUB1-conjugating enzyme, RCE1, reveals a requirement for RUB modification for control of ethylene biosynthesis and proper induction of basic chitinase and PDF1.2 in Arabidopsis.
By screening etiolated Arabidopsis seedlings for mutants with aberrant ethylene-related phenotypes, we identified a mutant that displays features of the ethylene-mediated triple response even in the absence of ethylene. Further characterization showed that the phenotype observed for the dark-grown seedlings of this mutant is reversible by prevention of ethylene perception and is dependent on a modest increase in ethylene production correlated with an increase in 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO) activity in the hypocotyl. Molecular characterization of leaves of the mutant revealed severely impaired induction of basic chitinase (chiB) and plant defensin (PDF)1.2 following treatment with jasmonic acid and/or ethylene. Positional cloning of the mutation resulted in identification of a 49-bp deletion in RCE1 (related to ubiquitin 1 (RUB1)-conjugating enzyme), which has been demonstrated to be responsible for covalent attachment of RUB1 to the SCF (Skpl Cdc 53 F-box) ubiquitin ligase complex to modify its activity. Our analyses with rce1-2 demonstrate a previously unknown requirement for RUB1 modification for regulation of ethylene biosynthesis and proper induction of defense-related genes in Arabidopsis.
Larsen
Paul B
PB
Department of Biochemistry, University of California-Riverside, Riverside, CA 92521, USA. paul.larsen@ucr.edu
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Jesse D
JD
D000594:Amino Acid Oxidoreductases / Q000235:genetics; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000096:biosynthesis*; D002688:Chitinases / Q000096:biosynthesis* / Q000235:genetics; D023082:Defensins / Q000096:biosynthesis* / Q000235:genetics; D005030:Ethylenes / Q000378:metabolism; D017343:Genes, Plant; D005808:Genes, Recessive; D018546:Hypocotyl / Q000201:enzymology; D008025:Ligases / Q000096:biosynthesis*; D010937:Plant Growth Regulators / Q000502:physiology; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis*; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*; D036226:Seedlings / Q000201:enzymology
D000594:Amino Acid Oxidoreductases; D029681:Arabidopsis Proteins; D002688:Chitinases; D023082:Defensins; D005030:Ethylenes; D008025:Ligases; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012273:Ribulose-Bisphosphate Carboxylase; C487542:At5g44420 protein, Arabidopsis; C036216:ethylene; C059460:1-aminocyclopropane-1-carboxylic acid oxidase; C487541:RCE1 protein, Arabidopsis