title: Stability indicating method for SPf66 antimalarial peptide in solution.
abstract: Stability studies on the SPf66 antimalarial peptide with different pH and temperature conditions were carried out. The degradation mechanism was elucidated by the size-exclusion chromatography (SEC) technique and the experimental data obtained at 37 degrees C and different pH were fitted to a kinetic degradation model that could explain the loss of its immunogenic capacity. At 5, 25, 37, and 70 degrees C and pH 2, changes were detected in the areas of the different species, although the values obtained could not be fitted to any known degradation kinetics.
authors.affiliation: Dpto. Ingeniería Química y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de La Laguna, Tenerife, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Santoveña
authors.forename: M J
authors.initials: MJ
authors.lastname: Dorta
authors.forename: A
authors.initials: A
authors.lastname: Oliva
authors.forename: M
authors.initials: M
authors.lastname: Llabrés
authors.forename: M E
authors.initials: ME
authors.lastname: Patarroyo
authors.forename: J B
authors.initials: JB
authors.lastname: Fariña
mesh_terms: D002850:Chromatography, Gel; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D017780:Malaria Vaccines / Q000737:chemistry*; D015800:Protozoan Proteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry*; D013696:Temperature; D014614:Vaccines, Synthetic / Q000737:chemistry
chemical_list: D017780:Malaria Vaccines; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C066269:SPf66 protein, Plasmodium; D014614:Vaccines, Synthetic