title: Mineralization of dentin induced by treatment with bioactive glass S53P4 in vitro.
abstract: Dentin hypersensitivity can be managed to occlude dentin tubules, but none of the agents used are components of natural dentin. Using a calcium phosphate precipitation (CPP) method, dentin tubules can be occluded with a calcium phosphate (CaP) layer similar to the major inorganic component of dentin. The CPP method utilizes acidic pH conditions, such as etching of dentin, over the course of several dental treatments. A gentler method can be used to produce a CaP layer on the surface of dentin. By treating with bioactive glass S53P4 (BAG), or regular commercial glass (CG), mineralization occurs in physiologically neutral solutions such as simulated body fluid (SBF) and remineralization solution (RMS). After a short period of immersion, silica is dissolved from both types of glass, but the amount of silica released is much greater from BAG than from CG. The dissolved silica is adsorbed on the surface of dentin during the pretreatment procedure and enhances the mineralization of dentin in SBF. After 14 days' mineralization the dentin is fully covered by the CaP layer, but after 14 days' immersion in RMS decalcification of the dentin occurs. Pretreatment with BAG decreases the degree of decalcification of dentin during the mineralization process. These findings suggest that bioactive glass S53P4 can be used as a therapeutic material for mineralization of dentin and its tubules in a physiological environment.
authors.affiliation: Turku Centre for Biomaterials, Turku, Finland. ari-pekka.forsback@utu.fi
authors.forename: Ari-Pekka
authors.initials: AP
authors.lastname: Forsback
authors.forename: Sami
authors.initials: S
authors.lastname: Areva
authors.forename: Jukka I
authors.initials: JI
authors.lastname: Salonen
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D032701:Biomimetics; D001826:Body Fluids / Q000737:chemistry; D002122:Calcium Chloride / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry; D003804:Dentin / Q000187:drug effects / Q000648:ultrastructure*; D003807:Dentin Sensitivity / Q000473:pathology; D005898:Glass / Q000737:chemistry*; D006801:Humans; D015636:Magnesium Chloride / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry; D011189:Potassium Chloride / Q000737:chemistry; D017680:Potassium Compounds / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D017693:Sodium Bicarbonate / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D012995:Solubility; D013431:Sulfates / Q000737:chemistry; D013997:Time Factors; D017001:Tooth Demineralization / Q000473:pathology; D014089:Tooth Remineralization
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D010710:Phosphates; D017680:Potassium Compounds; D013431:Sulfates; C485828:alpha-tricalcium phosphate; C403922:bioactive glass S53P4; C485830:tetracalcium phosphate; D015636:Magnesium Chloride; C012036:sodium sulfate; D012965:Sodium Chloride; D011189:Potassium Chloride; C485838:calcium phosphate, monobasic, anhydrous; D012822:Silicon Dioxide; D017693:Sodium Bicarbonate; C020243:calcium phosphate; C013216:potassium phosphate; C485829:calcium phosphate, dibasic, anhydrous; D002122:Calcium Chloride