title: Molecular mechanism of chemoresistance to cisplatin in ovarian cancer cell lines.
abstract: It is important to clarify the mechanism of resistance to cisplatin for the treatment of solid tumors. We have examined the expression of apoptosis-related proteins, Bax and Bcl-2, in ovarian cancer cells. We used the cell line 2008 and its cisplatin resistant subclone 2008 C-13. The percentage of 2008 cells showing apoptosis was significantly higher following cisplatin treatment as compared to untreated controls. 2008 C-13 cells showing apoptosis did not differ between the cisplatin-treated group and the untreated group. The expression of mRNA and protein of Bax in the two cell lines were not altered by treatment with cisplatin. Although the expression of mRNA and protein of Bcl-2 decreased in 2008 cells after treatment with cisplatin, the expression of Bcl-2 remained unchanged in 2008 C-13 cells. Our results indicate that the down-regulation of Bcl-2 plays an important role in the mechanisms of tumor resistance to anticancer drugs.
authors.affiliation: Departments of Gastroenterological Surgery, Transplant, and Surgical Oncology, Okayama University, Okayama 700-8558, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Murata
authors.forename: Minoru
authors.initials: M
authors.lastname: Haisa
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Uetsuka
authors.forename: Tetuji
authors.initials: T
authors.lastname: Nobuhisa
authors.forename: Takaomi
authors.initials: T
authors.lastname: Ookawa
authors.forename: Yoko
authors.initials: Y
authors.lastname: Tabuchi
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Shirakawa
authors.forename: Tomoki
authors.initials: T
authors.lastname: Yamatsuji
authors.forename: Junji
authors.initials: J
authors.lastname: Matsuoka
authors.forename: Masahiko
authors.initials: M
authors.lastname: Nishiyama
authors.forename: Noriaki
authors.initials: N
authors.lastname: Tanaka
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Naomoto
mesh_terms: D017209:Apoptosis / Q000502:physiology; D002945:Cisplatin / Q000494:pharmacology*; D015536:Down-Regulation / Q000502:physiology; D019008:Drug Resistance, Neoplasm / Q000502:physiology*; D005260:Female; D019254:Genes, bcl-2 / Q000502:physiology*; D006801:Humans; D010051:Ovarian Neoplasms / Q000188:drug therapy*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D051028:bcl-2-Associated X Protein
chemical_list: C494826:BAX protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D002945:Cisplatin