Effects of Cordyceps militaris extract on angiogenesis and tumor growth.
OBJECTIVE
To evaluate the effects of Cordyceps militaris extract (CME) on angiogenesis and tumor growth.


METHODS
Human umbilical vein endothelial cells (HUVEC), HT1080, and B16-F10 cells were used. DNA fragment, angiogenic related gene expressions (MMPs, bFGF, VEGF, etc), capillary tube formation, wound healing in vitro, tumor growth in vivo were measured.


RESULTS
CME inhibited growth of HUVECs and HT1080 (P<0.01). CME 100 and 200 mg/L reduced MMP-2 gene expression in HT1080 cells by 6.0 % and 22.9 % after 3-h and 14.9 % and 32.8 % after 6-h treatment. CME did not affect MMP-9 gene expression in B16-F10 melanoma cells. CME 100 and 200 mg/L also reduced bFGF gene expression in HUVECs by 22.2 % and 41.3 %. CME inhibited tube formation of endothelial cells in vitro and in vivo. CME repressed the growth of B16-F10 melanoma cells in mice compared with control group (P<0.05).


CONCLUSIONS
CME has antiangiogenetic properties.
Yoo
Hwa-seung
HS
Department of East-West Cancer Center, College of Oriental Medicine, Daejeon University, Daejeon 301-724, Korea.
Shin
Jang-woo
JW
Cho
Jung-hyo
JH
Son
Chang-gue
CG
Lee
Yeon-weol
YW
Park
Sang-yong
SY
Cho
Chong-kwan
CK
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D032661:Cordyceps / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis* / Q000235:genetics; D005354:Fibrosarcoma / Q000473:pathology; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000235:genetics; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics; D014407:Tumor Cells, Cultured; D014471:Umbilical Veins / Q000166:cytology
D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9