title: Use of the omp50 gene for identification of Campylobacter species by PCR.
abstract: We studied the prevalence of the omp50 gene and the Omp50 protein in Campylobacter strains. Immunodetection assays and DNA-DNA hybridizations showed that most C. coli strains tested were negative and most C. jejuni and C. lari strains tested were positive. A PCR assay was developed, using the omp50 gene as a species-specific target. We propose a combination of a hippurate test and an omp50 assay to perform identification of Campylobacter species.
authors.affiliation: EA 2197 and IFR 48, Faculté de Médecine, Université de la Méditerranée, 13385 Marseille Cedex 5, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Dedieu
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Pagès
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Bolla
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D002167:Campylobacter / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017000:Campylobacter coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D044863:Campylobacter lari / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D018272:Porins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D018272:Porins