An anti E. coli O157:H7 antibody-immobilized microcantilever for the detection of Escherichia coli (E. coli).
A silicon microcantilever sensor was developed for the detection of Escherichia coli O157:H7. The microcantilever was modified by anti-E. coli O157:H7 antibodies on the silicon surface of the cantilever. When the aquaria E. coli O157:H7 positive sample is injected into the fluid cell where the microcantilever is held, the microcantilever bends upon the recognition of the E. coli O157:H7 antigen by the antibodies on the surface of the microcantilever. A negative control sample that does not contain E. coli O157:H7 antigen did not cause any bending of the microcantilever. The detection limit of the sensor was 1 x 10(6) cfu/mL when the assay time was < 2 h.
Zhang
Jing
J
Department of Chemistry, Institute for Micromanufacturing, Louisiana Tech University, Ruston, Louisiana 71272, USA.
Ji
Hai-Feng
HF
D000327:Adsorption; D000907:Antibodies, Bacterial / Q000737:chemistry*; D003114:Colony-Forming Units Assay; D004926:Escherichia coli / Q000737:chemistry*; D019453:Escherichia coli O157 / Q000737:chemistry*
D000907:Antibodies, Bacterial