Beta-endorphin-like peptide SLTCLVKGFY reduces the production of 11-oxycorticosteroids by rat adrenal cortex through nonopioid beta-endorphin receptors.
Beta-endorphin-like peptide immunorphin (SLTCLVKGFY), a selective agonist of nonopioid beta-endorphin receptor, was labeled with tritium to specific activity of 24 Ci/mmol. It was used for the detection and characterization of nonopioid beta-endorphin receptors on rat adrenal cortex membranes (Kd = 31.6 +/- 0.2 nM, Bmax = 37.4 +/- 2.2 pmol/mg protein). Immunorphin at concentrations of 10(-9) to 10(-6) M was found to inhibit the adenylate cyclase activity in adrenal cortex membranes, while intramuscular injection of immunorphin at doses of 10-100 microg/kg was found to reduce the secretion of 11-oxycorticosteroids from the adrenals to the bloodstream.
Navolotskaya
Elena V
EV
Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Science Avenue 6, Pushchino, Moscow 142290, Russia. navolots@fibkh.serpukhov.su
Kovalitskaya
Yulia A
YA
Zolotarev
Yury A
YA
Kudryashova
Nina Yu
NY
Goncharenko
Elena N
EN
Kolobov
Alexander A
AA
Kampe-Nemm
Elena A
EA
Malkova
Natalia V
NV
Yurovsky
Vladimir V
VV
Lipkin
Valery M
VM
D000067956:Adenylyl Cyclase Inhibitors; D000302:Adrenal Cortex / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D000305:Adrenal Cortex Hormones / Q000096:biosynthesis* / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D007127:Immunoglobulin Constant Regions; D007142:Immunoglobulin gamma-Chains; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D011957:Receptors, Opioid / Q000819:agonists* / Q000378:metabolism; D001615:beta-Endorphin / Q000494:pharmacology*
D000067956:Adenylyl Cyclase Inhibitors; D000305:Adrenal Cortex Hormones; D007127:Immunoglobulin Constant Regions; D007142:Immunoglobulin gamma-Chains; D009842:Oligopeptides; D010446:Peptide Fragments; D011957:Receptors, Opioid; C445661:immunorphin; D001615:beta-Endorphin