Specific PCR assay for a tannin-tolerant selenomonas ruminantium isolate, derived from helicase coding sequences.
Sequences from a tannin-tolerant Selenomonas ruminantium isolate (EAT2) that hydrolyzes gallic acid were identified. Two exhibited identity to helicases with a wide phylogenetic distribution. PCR amplification by using primers from one helicase gene detected 2000 to 5000 EAT2 genome equivalents but did not amplify total gastrointestinal microbial DNA of nine other ungulate species.
Bishop
Richard
R
The International Livestock Research Institute, Nairobi, Kenya, and Addis Ababa, Ethiopia. R.Bishop@cgiar.org
Obura
Moses
M
Odongo
David
D
Odenyo
Agnes
A
D000818:Animals; D000835:Animals, Wild; D004265:DNA Helicases / Q000235:genetics* / Q000378:metabolism; D005707:Gallic Acid / Q000378:metabolism*; D006041:Goats; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012417:Rumen / Q000382:microbiology; D012418:Ruminants / Q000378:metabolism / Q000382:microbiology*; D020357:Selenomonas / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012756:Sheep; D013045:Species Specificity; D013634:Tannins / Q000378:metabolism*
D013634:Tannins; D005707:Gallic Acid; D004265:DNA Helicases