Dairy cows experience selective reduction of the hepatic growth hormone receptor during the periparturient period.
At parturition, dairy cows experience a 70% reduction in plasma IGF-I. This reduction coincides with decreased abundance of GHR1A, the liver-specific transcript of the growth hormone receptor (GHR) gene, suggesting impaired growth hormone-dependent synthesis of IGF-I. It is not immediately obvious that the periparturient reduction in GHR1A is sufficient to reduce hepatic GHR abundance. This is because approximately 50% of total GHR mRNA abundance in prepartum liver is accounted for by ubiquitously expressed transcripts which remain collectively unchanged at parturition. In addition, the possibility that parturition alters GHR expression in other growth hormone target tissue has not been examined. To address these questions, we measured GHR gene expression and GHR protein in liver and skeletal muscle of four dairy cows on days -35,+3 and+56 (relative to parturition on day 0). Hepatic GHR abundance and GHR1A transcripts were lower on day+3 than on day -35 and returned to late pregnancy value by day+56. Additional studies in two other groups of cows indicated that the hepatic levels of the GHR protein recovered substantially within 10 days after parturition. These changes occurred without variation in the abundance of HNF4, a liver-enriched transcription factor activating the promoter responsible for GHR1A synthesis. In contrast to liver, levels of GHR gene expression and GHR protein were identical on days -35,+3 and+56 in skeletal muscle. These data suggest a role for the GHR in regulating tissue-specific changes in growth hormone responsiveness in periparturient dairy cows.
Wook Kim
Jin
J
Department of Animal Science, Cornell University, Ithaca, New York 14853-4801, USA.
Rhoads
Robert P
RP
Block
Stephanie S
SS
Overton
Thomas R
TR
Frank
Stuart J
SJ
Boisclair
Yves R
YR
D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002417:Cattle / Q000378:metabolism*; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000032:analysis; D005260:Female; D015870:Gene Expression; D051557:Hepatocyte Nuclear Factor 4; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007743:Labor, Obstetric / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism; D010750:Phosphoproteins / Q000032:analysis; D049590:Postpartum Period / Q000378:metabolism; D011233:Precipitin Tests / Q000379:methods; D011247:Pregnancy; D043562:Receptors, G-Protein-Coupled / Q000032:analysis / Q000235:genetics; D054440:Receptors, Ghrelin; D011986:Receptors, Somatotropin / Q000032:analysis* / Q000378:metabolism; D014157:Transcription Factors / Q000032:analysis
D004268:DNA-Binding Proteins; D051557:Hepatocyte Nuclear Factor 4; D010750:Phosphoproteins; D043562:Receptors, G-Protein-Coupled; D054440:Receptors, Ghrelin; D011986:Receptors, Somatotropin; D014157:Transcription Factors; D007334:Insulin-Like Growth Factor I