title: [Preparation and identification of phage-displayed ICAM-1-mimic peptide].
abstract: OBJECTIVE
  To obtain bioactive ICAM-1 mimetic peptide.
  
  
  METHODS
  Phages displaying P1 and P2 were prepared by phage amplication and PEG precipitation. The binding between phage-displayed peptides and anti-ICAM-1 mAb 15.2 was evaluated by sandwich ELISA and competitive ELISA. Bioactivities of P1 and P2 was detected by immunohistochemical staining.
  
  
  RESULTS
  Phage-displayed peptides P1 and P2 could specifically bind to mAb 15.2, and the binding could be competitively inhibited by ICAM-1. Immunohistochemical staining showed that P1 and P2 could mimic the binding of ICAM-1 to its receptor LFA-1.
  
  
  CONCLUSIONS
  Phage-displayed peptides P1 and P2 are bioactive just as native ICAM-1.
authors.affiliation: Institute of Tropical Medicine, First Military Medical University, Guangzhou 510515, China. haowenbo@hotmail.com
authors.forename: Wen-bo
authors.initials: WB
authors.lastname: Hao
authors.forename: Wei-wen
authors.initials: WW
authors.lastname: Xu
authors.forename: Bai-hong
authors.initials: BH
authors.lastname: Chen
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Wen-qi
authors.initials: WQ
authors.lastname: Dong
authors.forename: Ming
authors.initials: M
authors.lastname: Li
mesh_terms: D000911:Antibodies, Monoclonal; D017101:Bacteriophage P1 / Q000235:genetics / Q000378:metabolism; D017901:Bacteriophage P2 / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D000911:Antibodies, Monoclonal; D016169:Lymphocyte Function-Associated Antigen-1; D019151:Peptide Library; D010455:Peptides; D018799:Intercellular Adhesion Molecule-1