Isolation and sequencing of the replication region of plasmid pBFp1 isolated from a marine biofilm.
A 24 kb plasmid, pBFp1, encoding mercury resistance was previously isolated from a marine biofilm. Isolation and sequencing of a 4280 bp DNA fragment containing the plasmid replicon (rep-pBFp1) revealed a putative open reading frame encoding a RepA protein and an oriV-like region containing an A+T rich sub-region, iterons, and DnaA boxes. Sequence comparisons showed significant similarities to the incW plasmid pSa both for the RepA amino acid sequence and in the iteron DNA sequence. Plasmid pBFp1 was also shown to be incompatible with pSa in standard incompatibility testing. A probe from the repA gene of pBFp1 was further made and tested on a collection of plasmids exogenously isolated from marine habitats in a previous study.
Bergström
Maria
M
Department of Cell and Molecular Biology, Microbiology Göteborg University, Box 462, SE 405 30, Sweden.
Hermansson
Malte
M
Dahlberg
Cecilia
C
D000595:Amino Acid Sequence; D001483:Base Sequence; D018441:Biofilms; D024881:Drug Resistance, Bacterial / Q000235:genetics; D008628:Mercury; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D016958:Pseudomonas putida / Q000235:genetics; D018741:Replication Origin / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D008628:Mercury