title: Root-end filling materials alter fibroblast differentiation.
abstract: Root-end filling materials are commonly used following endodontic surgical procedures; however, their effect on adjacent soft tissues is poorly understood. We predict that, due to the differences in their chemical composition, these materials will have profoundly different effects on the survival and differentiation of fibroblasts. Many of the root-end filling materials examined were initially cytotoxic to both PDL and gingival fibroblasts in co-culture experiments; however, this was reduced after the materials were washed in either mineral trioxide aggregate (MTA) or hybrid ionomere composite resin (HICR) for 2 wks. Additionally, PDL fibroblasts displayed enhanced proliferation on MTA and survival on amalgam when compared with gingival fibroblasts. MTA preferentially induced alkaline phosphatase expression and activity in both PDL and gingival fibroblasts. In contrast, HICR inhibited alkaline phosphatase expression and activity. In addition, MTA and HICR repressed pleiotrophin in PDL fibroblasts, while HICR repressed periostin in both fibroblasts. Thus, root-end filling materials differentially affect periodontal fibroblast differentiation.
  
  
  BACKGROUND
  mineral trioxide aggregate (MTA), zinc-oxide eugenol cement (ZOEC), hybrid ionomer composite resin (HICR), reverse-transcriptase polymerase chain-reaction (RT-PCR).
authors.affiliation: Department of Endodontics, Center of Excellence in Oral and Craniofacial Biology, Louisiana State University Health Science Center, School of Dentistry, New Orleans, LA 70119, USA.
authors.forename: S
authors.initials: S
authors.lastname: Bonson
authors.forename: B G
authors.initials: BG
authors.lastname: Jeansonne
authors.forename: T E
authors.initials: TE
authors.lastname: Lallier
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D017607:Aluminum Compounds / Q000633:toxicity; D015415:Biomarkers / Q000032:analysis; D017610:Calcium Compounds / Q000633:toxicity; D002352:Carrier Proteins / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis; D003722:Dental Alloys / Q000737:chemistry; D003723:Dental Amalgam / Q000737:chemistry; D017220:Dentin-Bonding Agents / Q000737:chemistry; D004338:Drug Combinations; D005347:Fibroblasts / Q000187:drug effects*; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D005899:Glass Ionomer Cements / Q000633:toxicity; D006133:Growth Substances / Q000032:analysis; D006801:Humans; D010087:Oxides / Q000633:toxicity; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D012117:Resins, Synthetic / Q000633:toxicity; D012184:Retrograde Obturation; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity; D015035:Zinc Oxide-Eugenol Cement / Q000633:toxicity
chemical_list: D017607:Aluminum Compounds; D015415:Biomarkers; D017610:Calcium Compounds; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D016207:Cytokines; D003722:Dental Alloys; D017220:Dentin-Bonding Agents; D004338:Drug Combinations; C074933:Geristore; D005899:Glass Ionomer Cements; D006133:Growth Substances; D010087:Oxides; C512835:POSTN protein, human; D012117:Resins, Synthetic; D012387:Root Canal Filling Materials; D017640:Silicates; D015035:Zinc Oxide-Eugenol Cement; C086631:mineral trioxide aggregate; C066845:pleiotrophin; C036886:tytin; D003723:Dental Amalgam; C084074:Super EBA; D000469:Alkaline Phosphatase