[Preparation and identification of phage-displayed ICAM-1-mimic peptide].
OBJECTIVE
To obtain bioactive ICAM-1 mimetic peptide.


METHODS
Phages displaying P1 and P2 were prepared by phage amplication and PEG precipitation. The binding between phage-displayed peptides and anti-ICAM-1 mAb 15.2 was evaluated by sandwich ELISA and competitive ELISA. Bioactivities of P1 and P2 was detected by immunohistochemical staining.


RESULTS
Phage-displayed peptides P1 and P2 could specifically bind to mAb 15.2, and the binding could be competitively inhibited by ICAM-1. Immunohistochemical staining showed that P1 and P2 could mimic the binding of ICAM-1 to its receptor LFA-1.


CONCLUSIONS
Phage-displayed peptides P1 and P2 are bioactive just as native ICAM-1.
Hao
Wen-bo
WB
Institute of Tropical Medicine, First Military Medical University, Guangzhou 510515, China. haowenbo@hotmail.com
Xu
Wei-wen
WW
Chen
Bai-hong
BH
Wang
Ping
P
Dong
Wen-qi
WQ
Li
Ming
M
D000911:Antibodies, Monoclonal; D017101:Bacteriophage P1 / Q000235:genetics / Q000378:metabolism; D017901:Bacteriophage P2 / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding
D000911:Antibodies, Monoclonal; D016169:Lymphocyte Function-Associated Antigen-1; D019151:Peptide Library; D010455:Peptides; D018799:Intercellular Adhesion Molecule-1