Plastic encapsulation of stabilized Escherichia coli and Pseudomonas putida.
Escherichia coli and Pseudomonas putida dried in hydroxyectoine or trehalose are shown to be highly resistant to the organic solvents chloroform and acetone, and consequently, they can be encapsulated in a viable form in solid plastic materials. Bacteria are recovered by rehydration after physical disruption of the plastic. P. putida incorporated into a plastic coating of maize seeds was shown to colonize roots efficiently after germination.
Manzanera
M
M
Institute of Biotechnology, University of Cambridge, Cambridge CB2 1QT, United Kingdom.
Vilchez
S
S
Tunnacliffe
A
A
D000096:Acetone / Q000494:pharmacology; D000599:Amino Acids, Diamino; D001709:Biotechnology / Q000379:methods*; D002725:Chloroform / Q000494:pharmacology; D003890:Desiccation; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development*; D010969:Plastics; D011137:Polystyrenes; D016958:Pseudomonas putida / Q000187:drug effects / Q000254:growth & development*; D012639:Seeds / Q000382:microbiology; D003313:Zea mays / Q000382:microbiology
D000599:Amino Acids, Diamino; D010969:Plastics; D011137:Polystyrenes; C121557:hydroxyectoine; D000096:Acetone; D002725:Chloroform