title: Qualitative and quantitative difference in mutation induction between carbon- and neon-ion beams in normal human cells.
abstract: We investigated the difference in cell-killing effect and mutation induction between carbon- and neon-ion beams in normal human cells. Carbon- and neon-ion beams were accelerated by the Riken Ring Cyclotron (RRC) at the Institute of Physical and Chemical Research in Japan. Cell-killing effect was measured as the reproductive cell death using the colony formation assay. Mutation induction at the HPRT locus was detected to measure 6-thioguanine-resistant clones. The mutation spectrum of the deletion pattern of exons of induced mutants was analyzed using the multiplex polymerase chain reaction (PCR). Cell-killing effect was almost the same between carbon- and neon-ion beams with similar linear energy transfer (LET) values, while there observed a large difference in mutation frequency. Furthermore, in the case of neon-ion beams 60% of mutants showed total deletions and 35-40% showed partial deletions, while 95-100% of carbon-ion induced mutants showed total deletions. The results suggest that different ion species may cause qualitative and quantitative difference in mutation induction even if the LET values are similar.
authors.affiliation: International Space Radiation Laboratory, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba-shi 263, Japan. m_suzuki@nirs.go.jp
authors.forename: Masao
authors.initials: M
authors.lastname: Suzuki
authors.forename: Chizuru
authors.initials: C
authors.lastname: Tsuruoka
authors.forename: Tatsuaki
authors.initials: T
authors.lastname: Kanai
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kato
authors.forename: Fumio
authors.initials: F
authors.lastname: Yatagai
authors.forename: Masami
authors.initials: M
authors.lastname: Watanabe
mesh_terms: D002244:Carbon; D016923:Cell Death / Q000528:radiation effects*; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects*; D002588:Cesium Radioisotopes; D017357:Cyclotrons; D004307:Dose-Response Relationship, Radiation; D004622:Embryo, Mammalian / Q000166:cytology / Q000528:radiation effects; D005720:Gamma Rays; D020450:Heavy Ions; D006801:Humans; D018499:Linear Energy Transfer; D016296:Mutagenesis; D009154:Mutation / Q000528:radiation effects*; D009356:Neon; D012062:Relative Biological Effectiveness
chemical_list: D002588:Cesium Radioisotopes; D009356:Neon; D002244:Carbon