Molecular fingerprinting of Mycobacterium bovis subsp. caprae isolates from central Europe.
To study the dissemination of Mycobacterium bovis subsp. caprae, 79 European isolates from cattle, humans, and other hosts were examined by spoligotyping and IS6110 restriction fragment length polymorphism (RFLP) analysis. Among a total of 11 different spoligotypes identified, type C1 proved to be predominant (n = 62). Five of the spoligotypes are described for the first time. A total of 43 different RFLP types were identified, thus allowing further differentiation for epidemiological tracking. Isolates from a series of outbreaks in one village proved to be of the same spoligotype and of identical or closely related RFLP types.
Erler
Wilfried
W
Federal Research Centre for Virus Diseases of Animals (BFAV), Jena, Germany.
Martin
Gerald
G
Sachse
Konrad
K
Naumann
Ludmila
L
Kahlau
Dagmar
D
Beer
Jörg
J
Bartos
Milan
M
Nagy
György
G
Cvetnic
Zeljko
Z
Zolnir-Dovc
Manca
M
Pavlik
Ivo
I
D000818:Animals; D002417:Cattle; D016172:DNA Fingerprinting; D005060:Europe; D019143:Evolution, Molecular; D006801:Humans; D017720:Molecular Epidemiology; D009163:Mycobacterium bovis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012150:Polymorphism, Restriction Fragment Length; D034421:Sus scrofa; D013553:Swine Diseases / Q000382:microbiology; D014376:Tuberculosis / Q000382:microbiology / Q000662:veterinary; D014380:Tuberculosis, Bovine / Q000382:microbiology