The effect of phalloidin and jasplakinolide on the flexibility and thermal stability of actin filaments.
In this work the effect of phalloidin and jasplakinolide on the dynamic properties and thermal stability of actin filaments was studied. Temperature dependent fluorescence resonance energy transfer measurements showed that filaments of Ca-actin became more rigid in the presence of phalloidin or jasplakinolide. Differential scanning calorimetric data implied that the stiffer filaments also had greater thermal stability in the presence of phalloidin or jasplakinolide. The fluorescence and calorimetric measurements provided evidences that the extent of stabilization by jasplakinolide was greater than that by phalloidin.
Visegrády
Balázs
B
Department of Biophysics, Faculty of Medicine, University of Pécs, Szigeti str. 12, Pécs H-7624, Hungary.
Lorinczy
Dénes
D
Hild
Gábor
G
Somogyi
Béla
B
Nyitrai
Miklós
M
D000199:Actins / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002151:Calorimetry; D002152:Calorimetry, Differential Scanning; D047630:Depsipeptides; D031541:Fluorescence Resonance Energy Transfer; D008856:Microscopy, Fluorescence; D018482:Muscle, Skeletal / Q000378:metabolism; D010456:Peptides, Cyclic / Q000494:pharmacology*; D010590:Phalloidine / Q000494:pharmacology*; D011487:Protein Conformation / Q000187:drug effects; D011817:Rabbits; D013696:Temperature; D013816:Thermodynamics
D000199:Actins; D000970:Antineoplastic Agents; D047630:Depsipeptides; D010456:Peptides, Cyclic; C057531:jasplakinolide; D010590:Phalloidine