Expression and functional role of serine/threonine phosphatases in rat esophagus.
BACKGROUND
Smooth muscle contraction is due to phosphorylation, while relaxation results from dephosphorylation of regulatory proteins such as the light chains of myosin (MLC20). Dephosphorylation of MLC20 is catalyzed by protein phosphatases (PP) types 1 and 2A. While some data are available on the expression and functional importance of PP1 and PP2A in vascular smooth muscle preparations, little is known of the importance of PP for force regulation in the gastrointestinal tract.


METHODS
Here we address the expression and functional importance of PP1 and PP2A in the rat esophagus. We measured both the isometric force of contraction and PP activity and also performed Western and Northern blotting for PPs in the tunica muscularis mucosae (TMM) and the muscularis mucosae propria (MM).


RESULTS
Cantharidin (0.01-100 microM) inhibited PP activity in homogenates from TMM (IC50=20 KM) in a concentration-dependent manner. The mRNAs of catalytic and regulatory subunits (PP1 alpha, PP1 beta, PP2A alpha and M110) were detectable at 1.8 kb, 3.2 kb, 2.0 kb and 5.7 kb in both TMM and MM. Western blot analysis revealed PP1 in TMM and MM. PP2A (protein) was more abundant in TMM than in TMM. Cantharidin increased muscular tone in isolated preparations of TMM (starting at 100 microM) to 164.6 (14.8 % of predrug force at 300 microM (the highest concentration studied). This increase in force of contraction was comparable to that of 10 microM carbachol.


CONCLUSIONS
The results suggest that PP activity can play an important role in the regulation of esophageal tone.
van der Voort
Ivo R
IR
Department of Medicine, Division of Hepatology, Charité-Universitätsmedizin Berlin, Campus Virchow-Klinikum, Humboldt-Universität zu Berlin, Germany. ivo.vandervoort@charite.de
Knapp
Jörg
J
Konturek
Jan W
JW
Boknik
Peter
P
Domschke
Wolfram
W
Schmitz
Wilhelm
W
Neumann
Joachim
J
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002193:Cantharidin / Q000737:chemistry; D002384:Catalysis; D020134:Catalytic Domain; D004064:Digestive System; D004305:Dose-Response Relationship, Drug; D004947:Esophagus / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D020128:Inhibitory Concentration 50; D009130:Muscle, Smooth / Q000166:cytology; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D009218:Myosins / Q000737:chemistry; D010749:Phosphoprotein Phosphatases / Q000096:biosynthesis / Q000378:metabolism / Q000502:physiology*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D010766:Phosphorylation; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D020033:Protein Isoforms; D012333:RNA, Messenger; D010749:Phosphoprotein Phosphatases; D010744:Phosphoric Monoester Hydrolases; D009218:Myosins; D002193:Cantharidin