title: p53 expression and environmental tobacco smoke exposure in feline oral squamous cell carcinoma.
abstract: The purpose of this study was to determine the prevalence of p53 overexpression in feline oral squamous cell carcinomas (SCC) and to determine, if any, the association between p53 overexpression and lifestyle factors and environmental exposures, including exposure to environmental tobacco smoke (ETS). Questionnaires concerning exposure to ETS and other environmental factors were sent to owners of cats presenting to the Harrington Oncology Program with a diagnosis of oral SCC between 1991 and 2000. Additionally, 23 formalin-fixed biopsy samples from these cats, with information regarding ETS, were evaluated immunohistochemically for p53 expression using the CM-1 clone and the avidin-biotin-horseradish peroxidase method. Of the 23 samples evaluated, 15 (65%) showed positive nuclear staining for the CM-1 clone. Tumor biopsy samples from cats exposed to any ETS were 4.5 times more likely to overexpress p53 than were tumors from unexposed cats (P = 0.19). Among cats with any ETS exposure, those with 5 years or longer of exposure were 7.0 times more likely to overexpress p53 (P = 0.38). Longhaired cats (P = 0.18) and female cats (P = 0.35) were also more likely to show p53 expression in their tumors. These results provide additional support for a relationship between oral SCC development and exposure to household ETS and may implicate p53 as a potential site for carcinogen-related mutation in this tumor.
authors.affiliation: Harrington Oncology Program, Tufts University School of Veterinary Medicine, 200 Westboro Road, North Grafton, MA 01536, USA.
authors.forename: L A
authors.initials: LA
authors.lastname: Snyder
authors.forename: E R
authors.initials: ER
authors.lastname: Bertone
authors.forename: R M
authors.initials: RM
authors.lastname: Jakowski
authors.forename: M S
authors.initials: MS
authors.lastname: Dooner
authors.forename: J
authors.initials: J
authors.lastname: Jennings-Ritchie
authors.forename: A S
authors.initials: AS
authors.lastname: Moore
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D002371:Cat Diseases / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D002415:Cats; D015870:Gene Expression; D016158:Genes, p53 / Q000502:physiology*; D007150:Immunohistochemistry; D009062:Mouth Neoplasms / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D011795:Surveys and Questionnaires; D014028:Tobacco Smoke Pollution
chemical_list: D014028:Tobacco Smoke Pollution