title: Enzymatic synthesis of two novel non-reducing oligosaccharides using transfructosylation activity with beta-fructofuranosidase from arthrobacter globiformis.
abstract: Two novel non-reducing oligosaccharides together with tri- and tetra-saccharides were synthesized by transfructosylation activity from sucrose as a donor and cellobiose or cellotriose as an acceptor with a purified beta-fructofuranosidase from Arthrobacter globiformis IFO 3062, and these oligosaccharides were identified as O-beta-D-glucopyranosyl-(1-->4)-alpha-D-glucopyranosyl-(1-->2)-alpha,beta-D-fructofuranoside and O-beta-D-glucopyranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->4)-alpha-D-glucopyranosyl-(1-->2)-alpha,beta-D-fructofuranoside by spectrometric analyses. Both oligosaccharides were stable under condition at 100 degrees C for 30 min, and showed no degradation at pH 2.
authors.affiliation: Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Than Than
authors.initials: TT
authors.lastname: Win
authors.forename: Naoto
authors.initials: N
authors.lastname: Isono
authors.forename: Yuyus
authors.initials: Y
authors.lastname: Kusnadi
authors.forename: Kenji
authors.initials: K
authors.lastname: Watanabe
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Obae
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ito
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Matsui
mesh_terms: D001173:Arthrobacter / Q000737:chemistry / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D010084:Oxidation-Reduction; D013395:Sucrose / Q000737:chemistry; D043324:beta-Fructofuranosidase / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D009844:Oligosaccharides; D013395:Sucrose; D043324:beta-Fructofuranosidase