Fractionation and anti-tumor activity of the mycelia of liquid-cultured Phellinus linteus.
All the fractions of Phellinus linteus mycelia showed anti-tumor activity toward solid tumors planted in mice. The highest anti-tumor activity of 81.2% was observed in the protein-glucan complex obtained by precipitating the 24% NaOH extract at pH 6.0. This protein-glucan complex consisted of 39.3% polysaccharide and 49.4% protein. Its (13)C- and (1)H-NMR data showed that the main glucan part of the complex was simple alpha-1,3-glucan chains.
Nakamura
Tomoyuki
T
Applied Fungi Institute, IBI Corporation, Yamanashi, Japan. n-tomo@comlink.ne.jp
Matsugo
Seiichi
S
Uzuka
Yasuyuki
Y
Matsuo
Sumitaka
S
Kawagishi
Hirokazu
H
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001487:Basidiomycota / Q000737:chemistry*; D002457:Cell Extracts / Q000737:chemistry; D045744:Cell Line, Tumor; D011232:Chemical Precipitation; D017077:Culture Media, Conditioned; D005936:Glucans / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D009368:Neoplasm Transplantation; D012510:Sarcoma 180 / Q000188:drug therapy / Q000473:pathology
D000970:Antineoplastic Agents; D002457:Cell Extracts; D017077:Culture Media, Conditioned; D005936:Glucans