Protective effect of doxorubicin induced heat shock protein 72 on cold preservation injury of rat livers.
OBJECTIVE
To observe the protective effect of heat shock protein 72 (HSP 72) induced by pretreatment of doxorubicin (DXR) on long-term cold preservation injury of rat livers.


METHODS
Sprague-Dawley rats were administered intravenously DXR at a dose of 1 mg/kg body mass in DXR group and saline in control group. After 48 h, the rat liver was perfused with cold Linger's and University of Wisconsin (UW) solutions and then was preserved in UW solution at 4 degrees for 24, 36 and 48 h. AST, ALT, LDH and hyaluronic acid in preservative solution were determined. Routine HE, immunohistochemical staining for HSP 72 and electron microscopic examination of hepatic tissues were performed.


RESULTS
After 24, 36 and 48 h, the levels of AST, ALT and hyaluronic acid in preservative solution were significantly higher in control group than in DXR group (P<0.05), while LDH level was not significantly different between the 2 groups (P>0.05). Hepatic tissues in DXR group were morphologically normal and significantly injured in control group. HSP 72 was expressed in hepatocytes and sinusoidal endothelial cells in DXR group but not in control group.


CONCLUSIONS
Pretreatment of DXR may extend the time of rat liver cold preservation and keep liver alive. The expression of HSP 72 in liver can prevent hepatocytes and sinusoidal endothelial cells from long-term cold preservation injury.
Chen
Hao
H
Department of Surgery and Center of Organ Transplantation, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China. chenhaodr@sohu.com
Yu
Ying-Yan
YY
Zhang
Ming-Jun
MJ
Deng
Xia-Xing
XX
Yang
Wei-Ping
WP
Ji
Jun
J
Peng
Cheng-Hong
CH
Li
Hong-Wei
HW
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000378:metabolism*; D003080:Cold Temperature; D015925:Cryopreservation / Q000379:methods*; D004317:Doxorubicin / Q000378:metabolism*; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism*; D008099:Liver; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D000903:Antibiotics, Antineoplastic; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D004317:Doxorubicin