Quantitative microbiological study of subgingival plaque by real-time PCR shows correlation between levels of Tannerella forsythensis and Fusobacterium spp.
A TaqMan-based real-time PCR assay was established to quantify the periodontopathic bacteria Tannerella forsythensis and Fusobacterium spp. With this assay, the prevalence and proportion of these bacteria in clinical specimens were evaluated. Our preliminary results suggest a positive colocalization of T. forsythensis and Fusobacterium spp. in periodontal pockets.
Suzuki
Nao
N
Department of Oral Bacteriology, Ohu University School of Dentistry, Tomitamachi, Koriyama 963-8611, Japan.
Yoshida
Akihiro
A
Saito
Toshiyuki
T
Kawada
Miki
M
Nakano
Yoshio
Y
D000328:Adult; D000368:Aged; D001431:Bacteriological Techniques; D041963:Bacteroidetes / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D003773:Dental Plaque / Q000382:microbiology*; D005673:Fusobacterium / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D004269:DNA, Bacterial