title: Role of Ca2+ in induction of neurotransmitter-related gene expression by butyrate.
abstract: We examined the effect of butyrate on neurotransmitter-related gene expression and calcium homeostasis in PC12 cells. Pretreatment with Ca2+ chelators (EGTA or BAPTA-AM) attenuated the butyrate-triggered accumulation of TH and ppEnk mRNA indicating that Ca2+ plays a role in butyrate-induced regulation of neuronal genes. Butyrate alone did not alter intracellular Ca2+ levels as determined by Fura-PE3 fluorescence; however, pretreatment with butyrate (18-24 h) reduced the first Ca2+ peak and prevented the second sustained rise in [Ca2+]i as induced by nicotine or ryanodine. In contrast, butyrate had no effect on Ca2+ transients when added shortly before or during nicotine or ryanodine stimulation. These results suggest that chronic butyrate exposure can modulate cell responses by affecting intracellular Ca2+ signaling.
authors.affiliation: Division of Newborn Medicine Westchester Medical Center/Department of Pediatrics; Department of Biochemistry and Molecular Biology, The Regional Neonatal Center, New York Medical College-Westchester Medical Center, Valhalla, NY 10595, USA.
authors.forename: Muhammad T
authors.initials: MT
authors.lastname: Zia
authors.forename: Bistra B
authors.initials: BB
authors.lastname: Nankova
authors.forename: Kavitha
authors.initials: K
authors.lastname: Krishnan
authors.forename: Volodia D
authors.initials: VD
authors.lastname: Gueorguiev
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Frenz
authors.forename: Esther L
authors.initials: EL
authors.lastname: Sabban
authors.forename: Edmund F
authors.initials: EF
authors.lastname: La Gamma
mesh_terms: D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D002118:Calcium / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000502:physiology; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D018377:Neurotransmitter Agents / Q000096:biosynthesis* / Q000378:metabolism; D016716:PC12 Cells; D051381:Rats
chemical_list: D002087:Butyrates; D018377:Neurotransmitter Agents; D002118:Calcium