title: Glutaraldehyde in bonding systems disinfects dentin in vitro.
abstract: OBJECTIVE
  To study the dentin disinfecting capacity of glutaraldehyde-containing bonding agents.
  
  
  METHODS
  Uniformly sized bovine dentin blocks infected with Enterococcus faecalis ATCC 29212 were treated according to manufacturer's instructions with a three-step bonding system (Syntac Classic), its single components, or Gluma Primer. Bacterial growth from dentinal filings obtained immediately after application of the bonding agents was assessed at different dentin depths. Minimal inhibitory concentration and minimal bactericidal concentration of glutaraldehyde against E. faecalis were determined.
  
  
  RESULTS
  Both Gluma Primer and Syntac Adhesive disinfected dentin, while the other components failed to do so. This was explained by the high antibacterial efficacy of glutaraldehyde even at low concentrations. Applied in consecutive order, the Syntac bonding system disinfected dentin blocks equally well with or without previous application of Gluma Primer.
  
  
  CONCLUSIONS
  Under the conditions of the current study, bonding agents containing glutaraldehyde appear to be good dentin disinfectants compatible with bonding systems. However, their effect on pulp tissue awaits further clarification.
authors.affiliation: Department of Preventive Dentistry, Cariology, and Periodontology, University of Zürich Center for Dental Medicine, Zürich, Switzerland. patrick.schmidlin@zzmk.unizh.ch
authors.forename: Patrick R
authors.initials: PR
authors.lastname: Schmidlin
authors.forename: Matthias
authors.initials: M
authors.lastname: Zehnder
authors.forename: Till N
authors.initials: TN
authors.lastname: Göhring
authors.forename: Tuomas M
authors.initials: TM
authors.lastname: Waltimo
mesh_terms: D000818:Animals; D002417:Cattle; D003804:Dentin / Q000187:drug effects* / Q000382:microbiology; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D004202:Disinfectants / Q000737:chemistry / Q000494:pharmacology*; D013293:Enterococcus faecalis / Q000187:drug effects; D005976:Glutaral / Q000737:chemistry / Q000494:pharmacology*; D008422:Materials Testing; D008826:Microbial Sensitivity Tests; D011109:Polymethacrylic Acids / Q000737:chemistry / Q000494:pharmacology; D019279:Resin Cements / Q000737:chemistry / Q000494:pharmacology
chemical_list: D017220:Dentin-Bonding Agents; D004202:Disinfectants; D011109:Polymethacrylic Acids; D019279:Resin Cements; C078484:Syntac dentine adhesive; C044372:Gluma; D005976:Glutaral