title: Synthesis and characterization of bifunctional probes for the specific labeling of fusion proteins.
abstract: Labeling proteins with synthetic probes is important for studying and characterizing protein function. We have recently introduced a general method for the specific in vivo and in vitro labeling of fusion proteins that is based on the reaction of O6-alkylguanine-DNA alkyltransferase (AGT) with O6-benzylguanine derivatives. Here we report two complementary routes for the synthesis of O6-benzylguanine derivatives, which allow for the labeling of AGT fusion proteins with bifunctional synthetic probes and demonstrate the specific labeling of AGT fusion proteins with these probes. These molecules should become useful tools for various applications in functional proteomics.
authors.affiliation: Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
authors.forename: Maik
authors.initials: M
authors.lastname: Kindermann
authors.forename: India
authors.initials: I
authors.lastname: Sielaff
authors.forename: Kai
authors.initials: K
authors.lastname: Johnsson
mesh_terms: D003001:Cloning, Molecular; D005982:Glutathione Transferase / Q000235:genetics; D006147:Guanine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D015335:Molecular Probes / Q000138:chemical synthesis* / Q000737:chemistry; D019853:O(6)-Methylguanine-DNA Methyltransferase / Q000737:chemistry / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D015335:Molecular Probes; D011993:Recombinant Fusion Proteins; D006147:Guanine; C008449:O-(6)-methylguanine; D019853:O(6)-Methylguanine-DNA Methyltransferase; D005982:Glutathione Transferase