Enzymatic synthesis of two novel non-reducing oligosaccharides using transfructosylation activity with beta-fructofuranosidase from arthrobacter globiformis.
Two novel non-reducing oligosaccharides together with tri- and tetra-saccharides were synthesized by transfructosylation activity from sucrose as a donor and cellobiose or cellotriose as an acceptor with a purified beta-fructofuranosidase from Arthrobacter globiformis IFO 3062, and these oligosaccharides were identified as O-beta-D-glucopyranosyl-(1-->4)-alpha-D-glucopyranosyl-(1-->2)-alpha,beta-D-fructofuranoside and O-beta-D-glucopyranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->4)-alpha-D-glucopyranosyl-(1-->2)-alpha,beta-D-fructofuranoside by spectrometric analyses. Both oligosaccharides were stable under condition at 100 degrees C for 30 min, and showed no degradation at pH 2.
Win
Than Than
TT
Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
Isono
Naoto
N
Kusnadi
Yuyus
Y
Watanabe
Kenji
K
Obae
Kazuhiro
K
Ito
Hiroyuki
H
Matsui
Hirokazu
H
D001173:Arthrobacter / Q000737:chemistry / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D010084:Oxidation-Reduction; D013395:Sucrose / Q000737:chemistry; D043324:beta-Fructofuranosidase / Q000737:chemistry* / Q000302:isolation & purification
D009844:Oligosaccharides; D013395:Sucrose; D043324:beta-Fructofuranosidase