Amplification of coccidioidal DNA in clinical specimens by PCR.
Coccidioides DNA was amplified from serum by a PCR using coccidioid-specific primers. A 239-bp product was visualized when 10 fg of exogenous coccidioidal DNA was subjected to amplification. This product was demonstrated in some human and mouse sera prior to the detection of coccidioidal antibodies.
Johnson
Suzanne M
SM
Department of Medical Microbiology and Immunology, School of Medicine, University of California-Davis, Davis, California 95616, USA. smjohnson@ucdavis.edu
Simmons
Keira A
KA
Pappagianis
Demosthenes
D
D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood; D003045:Coccidioides / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D003047:Coccidioidomycosis / Q000097:blood / Q000175:diagnosis / Q000382:microbiology; D004271:DNA, Fungal / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid / Q000235:genetics*; D006801:Humans; D051379:Mice; D021141:Nucleic Acid Amplification Techniques / Q000706:statistics & numerical data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D012698:Serologic Tests
D000908:Antibodies, Fungal; D004271:DNA, Fungal