title: Protein-coated poly(L-lactic acid) fibers provide a substrate for differentiation of human skeletal muscle cells.
abstract: Tissue engineering represents a potential method for repairing damaged skeletal muscle tissue. Extracellular matrix (ECM) proteins were evaluated for their ability to aid in cell attachment, whereas a poly(L-lactic acid) (PLLA) fiber scaffold was tested as a substrate for the differentiation of human skeletal muscle cells. In comparison to uncoated or gelatin-coated PLLA films, cell attachment increased significantly (p < 0.001) on PLLA films coated with ECM gel, fibronectin, or laminin. Myoblasts differentiated into multinucleated myofibers on ECM gel-coated PLLA fibers, and expressed muscle markers such as myosin and alpha-actinin. Oligonucleotide microarray analysis showed similar gene expression profiles for human skeletal muscle cells on ECM gel-coated PLLA fibers as to that observed for myofibers on tissue culture plates. Therefore, PLLA fibers coated with ECM proteins provide a scaffold for the development of skeletal muscle tissue for tissue engineering and cell transplantation applications.
authors.affiliation: Joint Biomedical Engineering Program, University of Texas Southwestern Medical Center, Dallas, and University of Texas at Arlington, Arlington, USA.
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Cronin
authors.forename: Frederick A
authors.initials: FA
authors.lastname: Thurmond
authors.forename: Rhonda
authors.initials: R
authors.lastname: Bassel-Duby
authors.forename: R Sanders
authors.initials: RS
authors.lastname: Williams
authors.forename: Woodring E
authors.initials: WE
authors.lastname: Wright
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Nelson
authors.forename: Harold R
authors.initials: HR
authors.lastname: Garner
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry / Q000378:metabolism*; D008422:Materials Testing; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology* / Q000502:physiology; D032448:Myoblasts, Skeletal / Q000166:cytology / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods
chemical_list: D016326:Extracellular Matrix Proteins; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)