Identification of the major vanilloid component in Capsicum extract by HPLC-EC and HPLC-MS.
A sensitive multi-channel HPLC-electrochemical (EC) method has been developed to determine the vanilloid content in the complex Capsicum annuum extract Capsibiol. Chromatographic separation was achieved within 10 min using a YMC Basic S5 column with a mobile phase containing chloroacetic acid, heptane sulphonic acid and acetonitrile. The multi-channel detector simultaneously applied four potentials between +500 and +800 mV (referenced to a silver/silver chloride electrode) to four glassy carbon working electrodes. The most abundant (0.94 mg/g) vanilloid analogue in the Capsibiol sample demonstrated an electrochemical reactivity and retention time similar to that of vanillic acid in HPLC-EC analysis. Its identity was confirmed by HPLC-MS using a Zorbax SB-CN column with a mobile phase containing formic acid and methanol.
Zhou
Qin
Q
Bioanalytical Systems Inc., 2701 Kent Avenue, West Lafayette, IN 47906, USA. qinz@bioanalytical.com
Zhu
Yongxin
Y
Chiang
Hwa
H
Yagiz
Kader
K
Morre
D James
DJ
Morre
Dorothy M
DM
Janle
Elsa
E
Kissinger
Peter T
PT
D002212:Capsicum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014641:Vanillic Acid / Q000032:analysis* / Q000737:chemistry
C502157:Capsibiol; D010936:Plant Extracts; D014641:Vanillic Acid