[Effect of PD98059 on Ras-MAPK signal transduction pathway of chronic myelogenous leukemia].
OBJECTIVE
To study the effect and mechanism of PD98059 on Ras-MAPK signal transduction pathway of chronic myelogenous leukemia.


METHODS
K562 cell line was treated by PD98059, cell viability, DNA synthesis, colony formation and MAPK activity of the treated cells were analyzed.


RESULTS
The cell viability, DNA synthesis, colony formation and MAPK activity were significantly inhibited by PD98059 (P<0.05), and the inhibitory effect was dose dependent.


CONCLUSIONS
The inhibitory effect of PD98059 was achieved by blocking Ras-MAPK signal transduction pathway which can become a new target in the treatment of CML.
Shang
Zhen-chuan
ZC
Hematology Department of Xijing Hospital, Fourth Military Medical University,Xi'an 710032,China.
Sun
Bing-zhong
BZ
Chen
Zhi-nan
ZN
Wang
Jing
J
Feng
Qi
Q
Wang
Wei
W
Wang
Sha
S
Zhang
Tao
T
D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D020014:K562 Cells; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D015398:Signal Transduction; D018631:ras Proteins / Q000378:metabolism*
D004273:DNA, Neoplasm; D005419:Flavonoids; D020928:Mitogen-Activated Protein Kinases; D018631:ras Proteins; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one