title: In vivo evidence suggesting a role for purine-catabolizing enzymes in the pathogenesis of cisplatin-induced nephrotoxicity in rats and effect of erdosteine against this toxicity.
abstract: The aim of this experimental study was to investigate the possible role of adenosine deaminase (AD) and xanthine oxidase (XO) in the pathogenesis of cisplatin-induced nephrotoxicity and the effect of erdosteine in decreasing the toxicity. The intraperitoneal injection of cisplatin (7 mg kg(-1) body weight) induced a significant increase in plasma creatinine level and blood urea nitrogen (BUN), and plasma and damaged renal tissue activities of AD and XO in rats. Co-treatment with erdosteine (10 mg kg(-1)day(-1)) attenuated the increase in the plasma creatinine and BUN levels, and significantly prevented the increase in tissue and plasma AD and XO activities (P<0.05). The results of this study revealed that XO and AD may play an important role in the pathogenesis of cisplatin-induced nephrotoxicity. The potent free radical scavenger erdosteine may have protective potential in this process and it will become a promising drug in the prevention of this undesired side-effect of cisplatin, but further studies are needed to illuminate the exact protection mechanism of erdosteine against cisplatin-induced nephrotoxicity.
authors.affiliation: Department of Biochemistry, Inonu University Medical Faculty, Malatya, Turkey.
authors.forename: Sadik
authors.initials: S
authors.lastname: Söğüt
authors.forename: Mahir
authors.initials: M
authors.lastname: Kotuk
authors.forename: H Ramazan
authors.initials: HR
authors.lastname: Yilmaz
authors.forename: Ramazan
authors.initials: R
authors.lastname: Ulu
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Ozyurt
authors.forename: Zeki
authors.initials: Z
authors.lastname: Yildirim
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced* / Q000201:enzymology / Q000517:prevention & control; D000243:Adenosine Deaminase / Q000032:analysis / Q000378:metabolism; D000818:Animals; D002945:Cisplatin / Q000037:antagonists & inhibitors / Q000633:toxicity*; D005260:Female; D011687:Purines / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013864:Thioglycolates / Q000627:therapeutic use*; D013876:Thiophenes / Q000627:therapeutic use*; D014969:Xanthine Oxidase / Q000032:analysis / Q000378:metabolism
chemical_list: D011687:Purines; D013864:Thioglycolates; D013876:Thiophenes; C048498:erdosteine; D014969:Xanthine Oxidase; D000243:Adenosine Deaminase; D002945:Cisplatin