Chiral separation of tamsulosin isomers by HPLC using cellulose tris (3,5-dimethhylphenylcarbamate) as a chiral stationary phase.
A high-performance liquid chromatographic (HPLC) method was developed for the chiral separation of an antagonist of alpha1A adrenoceptors, tamsulosin and its S-isomer. Baseline separation of the isomers was achieved within 35 min on a CHIRALCEL OD-RH column with a binary solvent mixture of 50 mmol l(-1) KPF6-acetonitrile (v/v (70:30), pH 5.0) as the optimized mobile phase. The detection limits and quantification limits of both R-isomer and S-isomer were 0.11 and 0.44 ng, respectively. The R.S.D. values of peak-area for the two isomer were 0.42% (of peak-height: 0.77%) for R-isomer and 0.64% (of peak-height:0.92%) for S-isomer (n = 5).
Zhang
Zhefeng
Z
Department of Chemistry, College of Chemistry and Environmental Science, Hebei University, Baoding, Hebei 071002, PR China.
Yang
Gengliang
G
Liang
Guijian
G
Liu
Haiyan
H
Chen
Yi
Y
D002219:Carbamates / Q000032:analysis* / Q000737:chemistry*; D002482:Cellulose / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D048448:Phenylcarbamates; D013237:Stereoisomerism; D013449:Sulfonamides / Q000032:analysis* / Q000737:chemistry*; D000077409:Tamsulosin
D002219:Carbamates; D048448:Phenylcarbamates; D013449:Sulfonamides; C065943:cellulose tris-3,5-dimethylphenyl-carbamate; D002482:Cellulose; D000077409:Tamsulosin