title: Site-specific conjugation of oligonucleotides to the C-terminus of recombinant protein by expressed protein ligation.
abstract: We developed a convenient method for synthesizing homogeneous DNA-protein conjugates. The method is based on expressed protein ligation of intein-fusion proteins and oligonucleotides derivatized with a cysteine. A range of cysteinyl oligonucleotides were synthesized by using a new reagent 1 and were successfully applied to expressed protein ligation to attach the oligonucleotides specifically at the C-terminus of a recombinant protein.
authors.affiliation: Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo 113-8656, Japan.
authors.forename: Shuji
authors.initials: S
authors.lastname: Takeda
authors.forename: Shinya
authors.initials: S
authors.lastname: Tsukiji
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Nagamune
mesh_terms: D000595:Amino Acid Sequence; D003432:Cross-Linking Reagents; D003545:Cysteine / Q000737:chemistry; D047668:Inteins; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry*
chemical_list: D003432:Cross-Linking Reagents; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D003545:Cysteine