Phagosomal acidification is not a prerequisite for intracellular multiplication of Legionella pneumophila in human monocytes.
Legionella pneumophila is able to multiply in a variety of eukaryotic cells. Contradictory results have been published on the significance of phagosomal acidification in the intracellular multiplication of Legionella species in monocytes. Therefore, we analyzed the phagosomal pH values in 2 different types of human monocytes throughout the intracellular-replication cycles of 2 Legionella species that have different rates of intracellular multiplication. Our results show that phagosomal acidification is not a prerequisite for intracellular replication of Legionella pneumophila in human monocytes.
Wieland
Hagen
H
Department of Transfusion Medicine, University Hospitals Tubingen, Tubingen, Germany. hagen.wieland@uni-tuebingen.de.
Goetz
Friedrich
F
Neumeister
Birgid
B
D015169:Colony Count, Microbial; D049452:Green Fluorescent Proteins; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016952:Legionella pneumophila / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D009000:Monocytes / Q000382:microbiology*; D010587:Phagocytosis; D010588:Phagosomes / Q000737:chemistry* / Q000382:microbiology
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins