title: Effects of ascorbic acid and ascorbic acid 2-phosphate, a long-acting vitamin C derivative, on the proliferation and differentiation of human osteoblast-like cells.
abstract: In order to investigate the effect of ascorbic acid (AsA) and ascorbic acid 2-phosphate (Asc 2-P), a long-acting vitamin C derivative, on the growth and differentiation of human osteoblast-like cells, we supplemented the culture medium of MG-63 cells with various concentrations (0.25 to 1 mM) of these factors. Asc 2-P significantly stimulated nascent cell growth at all concentrations in the presence of fetal bovine serum (FBS). On the other hand, AsA showed a growth repressive effect depending on its concentration, and that of FBS. Asc 2-P also increased expression of osteoblast differentiation markers, such as collagen synthesis and alkaline phosphatase (ALP) activity. These stimulative activities of Asc 2-P were attenuated by inhibitors of collagen synthesis, indicating that these effects were dependent on collagen synthesis. Electron micrographs of the cells showed the formation of a three-dimensional tissue-like structure endowed with a mature extracellular matrix in the presence of Asc 2-P.
authors.affiliation: Department of Biochemistry and Molecular Biology, Kanagawa Dental College, 82 Inaoka-cho, Yokosuka 238-8580, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Takamizawa
authors.forename: Yojiro
authors.initials: Y
authors.lastname: Maehata
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Imai
authors.forename: Haruki
authors.initials: H
authors.lastname: Senoo
authors.forename: Sadao
authors.initials: S
authors.lastname: Sato
authors.forename: Ryu-Ichiro
authors.initials: R
authors.lastname: Hata
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D001383:Azetidinecarboxylic Acid / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D006801:Humans; D046529:Microscopy, Electron, Transmission; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
chemical_list: C011669:ascorbate-2-phosphate; D001383:Azetidinecarboxylic Acid; D003094:Collagen; D000469:Alkaline Phosphatase; D001205:Ascorbic Acid