title: [Study on the relationship between nitric oxide synthase gene G894T polymorphism and hypertension related risk factors in patients with essential hypertension in Chongqing city].
abstract: OBJECTIVE
  To explore the relationship between genetic anomaly and risk factors in hypertension, a polymorphism at position G894T of the gene encoding the endothelial nitric oxide synthase (eNOS) together with hypertension related risk factors were observed in patients with essential hypertension (EH) in Chongqing city.
  
  
  METHODS
  Two hundred and twenty-six patients with EH and matched controls were selected. Genotypes of polymorphisms were determined by polymerase chain reaction (PCR), while PCR products were digested by restriction endonuclease (BanII). Questionnaire referred to life style, dietary, smoking, alcohol consumption, psychological and mental state, waist-to-hip ratio (WHR), etc was administered.
  
  
  RESULTS
  There was no significant difference noticed in genotype distribution for the eNOS gene G894T genotype between hypertensive groups and controls, but difference was found among certain related risk factors, such as salt intake, snoring and WHR, etc. Logistic regression analysis showed no association between 894T allele and hypertension.
  
  
  CONCLUSIONS
  Although the polymorphism of eNOS gene G894T did not seem to play an important and direct role in the pathogenesis of EH it might have indirect effects through certain risk factors.
authors.affiliation: Daping Hospital, The Third Military Medical University, Chongqing 400042, China.
authors.forename: Jian-cong
authors.initials: JC
authors.lastname: Tan
authors.forename: Zhi-ming
authors.initials: ZM
authors.lastname: Zhu
authors.forename: Shan-jun
authors.initials: SJ
authors.lastname: Zhu
authors.forename: Chang-qing
authors.initials: CQ
authors.lastname: Yu
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Liu
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
mesh_terms: D000328:Adult; D002681:China; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000201:enzymology / Q000235:genetics*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D019001:Nitric Oxide Synthase / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors; D011795:Surveys and Questionnaires
chemical_list: D019001:Nitric Oxide Synthase