Effects of elephant garlic volatile oil (Allium ampeloprasum) and T-2 toxin on murine skin.
Effects of elephant garlic (Allium ampeloprasum) volatile oil (GVO) and trichothecene (T-2) toxin were studied in Swiss albino mice. The animals were 1) topically applied GVO, 2) topically applied T-2 toxin, 3) topically applied GVO followed by T-2 toxin (GVO/T-2), and 4) T-2 toxin application followed by GVO (T-2/GVO) on the right footpad. All animals were observed by Langerhans cell enumeration and pathological changes of the footpad on days 1, 3, 5 and 7. The number of Langerhans cells in the GVO treated group (1,097 +/- 33/mm2 to 1,624 +/- 19/mm2) was not significantly different when compared with the corresponding control left footpad (1,143 +/- 33/mm2 to 1,674 +/- 21/mm2). Langerhans cells density in T-2 toxin treated group (629 +/- 29/mm2to 1,090 +/- 31/mm2) was reduced by 20-35% of the opposite control footpad (962 +/- 40/mm2 to 1,392 +/- 29/mm2). Furthermore, GVO/T-2 and T-2/GVO treated mice showed a decrease in Langerhans cell number than a single T-2 toxin treated group. While Langerhans cells in T-2 toxin, GVO/T-2 and T-2/GVO groups revealed a smaller cell size with shortening dendritic processes when compare to the normal control group. Histopathological findings of the footpad skin in T-2 toxin treated group revealed epidermal desquamation and necrosis with edema and inflammatory cells infiltration. While GVO/T-2 and T-2/GVO showed a similar sequence but a lesser severe degree. These findings suggested that GVO both in pre- and posttreatment could protect T-2 toxin induced epidermal damage in a mouse footpad.
Nguansangiam
Sudarat
S
Department of Pathology, Bangkok Metropolitan Administration Medical College and Vajira Hospital, Bangkok, Thailand.
Angsubhakorn
Subhkij
S
Bhamarapravati
Sutatip
S
Suksamrarn
Apichart
A
D000818:Animals; D005737:Garlic; D007801:Langerhans Cells / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D009822:Oils, Volatile / Q000494:pharmacology*; D010938:Plant Oils / Q000494:pharmacology*; D011897:Random Allocation; D012867:Skin / Q000187:drug effects* / Q000473:pathology; D013605:T-2 Toxin / Q000494:pharmacology*
D009822:Oils, Volatile; D010938:Plant Oils; D013605:T-2 Toxin