Validated 5' nuclease PCR assay for rapid identification of the genus Brucella.
A real-time, genus-specific 5' nuclease PCR assay for amplification of a 322-bp fragment of the per gene was developed for rapid (<2 h) identification of Brucella spp. from agar plates. The assay, including an internal amplification control (116 bp), identified Brucella strains (n = 23) and did not detect non-Brucella strains (n = 174), indicating its usefulness, particularly for laboratories with stringent quality assurance programs.
Bogdanovich
T
T
University of Turku, Turku, Finland.
Skurnik
M
M
Lübeck
P S
PS
Ahrens
P
P
Hoorfar
J
J
D000818:Animals; D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D001483:Base Sequence; D002002:Brucella / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification*; D002006:Brucellosis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary; D002238:Carbohydrate Epimerases / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D000637:Transaminases / Q000235:genetics
D017931:DNA Primers; D004269:DNA, Bacterial; D000637:Transaminases; D002238:Carbohydrate Epimerases; C061272:perosamine synthetase