title: Interaction between a type-II dockerin domain and a type-II cohesin domain from Clostridium thermocellum cellulosome.
abstract: The interaction between the type-II dockerin domain of the scaffoldin protein CipA and the type-II cohesin domain of the outer layer protein SdbA is the fundamental mechanism for anchoring the cellulosome to the cell surface of Clostridium thermocellum. We constructed and purified a dockerin polypeptide and a cohesin polypeptide, and determined affinity constants of the interaction between them by the surface plasmon resonance method. The dissociation constant (K(D)) value was 1.8 x 10(-9) M, which is a little larger than that for the combination of a type-I dockerin and a type-I cohesin.
authors.affiliation: Faculty of Bioresources, Mie University, Tsu, Japan.
authors.forename: Sadanari
authors.initials: S
authors.lastname: Jindou
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kajino
authors.forename: Minoru
authors.initials: M
authors.lastname: Inagaki
authors.forename: Shuichi
authors.initials: S
authors.lastname: Karita
authors.forename: Pierre
authors.initials: P
authors.lastname: Beguin
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kimura
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sakka
authors.forename: Kunio
authors.initials: K
authors.lastname: Ohmiya
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D018797:Cell Cycle Proteins; D044603:Cellulosomes / Q000737:chemistry* / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone; D003013:Clostridium / Q000737:chemistry* / Q000378:metabolism; D005656:Fungal Proteins; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D009687:Nuclear Proteins / Q000737:chemistry*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D020349:Surface Plasmon Resonance; D000097722:Cohesins
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D005656:Fungal Proteins; D008565:Membrane Proteins; D009687:Nuclear Proteins; D000097722:Cohesins; C080369:CipA protein, Clostridium; C093327:OlpB protein, Clostridium thermocellum; C105449:SdbA protein, bacteria