Prevalence of non-O157 Escherichia coli strains among shiga-like toxin-producing (SLTEC) isolates in the region of Lower Silesia, Poland.
During 5 y (1997-2002) in the region of Lower Silesia, Poland, 55 shiga-like toxin-producing strains were isolated from children with diarrhoea, none of whom developed haemolytic uremic syndrome. Shiga-like toxin production was detected on Vero cells and confirmed by the detection of slt1 and slt2 gene sequences. Most of isolates examined possessed slt2 gene alone (41.8%), or in combination with slt1 gene (38.2%). None of the shiga-like toxin-producing strains was of serotype O157:H7. It appears that non-O157 shiga-like toxin-producing E. coil strains are prevalent as the causal agent of severe diarrhoea in the region.
Sobieszczańska
Beata
B
Department of Microbiology, University of Medicine, 4 Chalubińskiego Street, 50-368 Wroclaw, Poland. mapasobie@provider.pl
Gryko
Romuald
R
Kuzko
Katarzyna
K
Dworniczek
Ewa
E
D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000302:isolation & purification* / Q000378:metabolism*; D005243:Feces / Q000382:microbiology; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011044:Poland / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D012424:Rural Population; D012494:Sampling Studies; D012720:Severity of Illness Index; D022622:Shiga Toxin 1 / Q000032:analysis*; D022641:Shiga Toxin 2 / Q000032:analysis*
D004269:DNA, Bacterial; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2