Enhancement of antitumor response to sarcoma 45 in rats by combination of whole-body hyperthermia and interleukin-2.
OBJECTIVE
To evaluate the summarized effect of hyperthermia and interleukin-2 (IL-2) administration on antitumor defense in tumor-bearing rats.


METHODS
Nonbred rats after subcutaneous inoculation of sarcoma 45 cells were treated with whole-body hyperthermia (WBH, +42.5 degrees C, 60 min) and interleukin-2 (IL-2, 10,000 U/kg of body weight). Parameters of tumor growth and survival of animals were monitored till day 33 after tumor cell inoculation.


RESULTS
Combined application of WBH and IL-2 at 5th day after tumor cell transplantation resulted in a delay of tumor growth and improvement of survival parameters in comparison with control group or animals that received separate treatment. Therapeutic efficacy of WBH combined with IL-2 was analogous to a single-dose chemotherapy with cyclophosphamide.


CONCLUSIONS
Combined application of WBH and IL-2 is the useful approach for cancer immunotherapy.
Potapnev
Michael P
MP
Research Department, Belarussian Research Center for Pediatric Oncology & Hematology, P.O. Lesnoy, Minsk BE-223052, Belarus. mpotapnev@yandex.ru
Istomin
Yuri P
YP
Ismail-zade
Reiman S
RS
Zhavrid
Edvard A
EA
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D003131:Combined Modality Therapy; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000627:therapeutic use*; D005260:Female; D006979:Hyperthermia, Induced; D007279:Injections, Subcutaneous; D007376:Interleukin-2 / Q000008:administration & dosage / Q000627:therapeutic use*; D009368:Neoplasm Transplantation; D051381:Rats; D012513:Sarcoma, Experimental / Q000276:immunology / Q000473:pathology / Q000628:therapy*
D000970:Antineoplastic Agents; D007376:Interleukin-2; D003520:Cyclophosphamide