Correlation between Helicobacter pylori OipA protein expression and oipA gene switch status.
Polyclonal antisera to either a synthetic OipA peptide or a recombinant OipA protein detected OipA expression in Helicobacter pylori and correlated with functional oipA status determined by PCR sequence (sensitivity and specificity of >94%). Immunoblotting is a simple and accurate method for detecting expression of the important virulence factor OipA.
Kudo
Takahiko
T
Department of Medicine, Baylor College of Medicine, Houston, Texas, USA.
Nurgalieva
Zhannat Z
ZZ
Conner
Margaret E
ME
Crawford
Sue
S
Odenbreit
Stefan
S
Haas
Rainer
R
Graham
David Y
DY
Yamaoka
Yoshio
Y
D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D016480:Helicobacter pylori / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D006801:Humans; D015151:Immunoblotting / Q000379:methods / Q000706:statistics & numerical data; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity; D014774:Virulence / Q000235:genetics
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D011994:Recombinant Proteins