title: Comparison of efficiency of ex vivo expansion of whole blood, mononuclear cells and purified CD34+ cells from human umbilical cord blood.
abstract: We used a dual-chamber culture system separated by a dialysis membrane to test the efficiency of expansion of whole cord blood and cell fractions. We found that expansion of progenitor cells was more efficient from whole blood than from purified CD34+ or partially purified mononuclear cell fractions.
authors.forename: Kyung-Ha
authors.initials: KH
authors.lastname: Ryu
authors.forename: Hee-Young
authors.initials: HY
authors.lastname: Shin
authors.forename: Hyo-Seop
authors.initials: HS
authors.lastname: Ahn
authors.forename: Young-Ju
authors.initials: YJ
authors.lastname: Kim
authors.forename: So-Youn
authors.initials: SY
authors.lastname: Woo
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Seoh
mesh_terms: D018952:Antigens, CD34 / Q000097:blood*; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000276:immunology
chemical_list: D018952:Antigens, CD34