[Studies on liver-toxicity in rhigoma of Dioscorea bulbifera].
OBJECTIVE
To explore the liver-toxic fraction in Rhigoma of Dioscorea bulbifera.


METHODS
The rats were randomized into four groups: control group (20% PVP-water), T001(10% total methanol extraction), F002(5% chloroform fraction) and F003(5% methanol fraction). Direct bilirubin (DBil) and Glutamic-pyruvic transaminase (GPT) were examined, and liver index was measured. The histological and morphological observations were performed with optical and electrical microscope.


RESULTS
T001 and F002 showed significant liver toxicity.


CONCLUSIONS
The chloroform fraction was the liver-toxic fraction of D. bulbifera.
Tan
Xing-qi
XQ
NO. 98 Hospital of PLA, Huzhou 313000, Zhejang, China. tanxq@sohu.com
Ruan
Jin-lan
JL
Chen
Hai-sheng
HS
Wang
Ju-ying
JY
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001663:Bilirubin / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000209:etiology / Q000473:pathology; D027742:Dioscorea / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000633:toxicity*; D005260:Female; D008099:Liver / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D000410:Alanine Transaminase; D001663:Bilirubin