Genetic variation in an endemic salamander, Salamandra atra, using amplified fragment length polymorphism.
The pattern of genetic differentiation of the endemic alpine salamander, Salamandra atra, has been studied using amplified fragment length polymorphism (AFLP) from 11 populations throughout the range of the two currently recognized subspecies, atra and aurorae. Five different primer combinations produced 706 bands and were analyzed by constructing a phylogenetic tree using NJ and principal component analysis. Significant genetic variation was revealed by AFLP between and within populations but, our results show a lack of genetic structure. AFLP markers seems to be unsuitable to investigate complex and recent diversification.
Riberon
Alexandre
A
Laboratoire de Biologie des populations d'Altitude, UMR CNRS 5553, Université de J. Fourier, Grenoble, France. riberon@cict.fr
Miaud
Claude
C
Guyetant
R
R
Taberlet
P
P
D000818:Animals; D017931:DNA Primers / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006239:Haplotypes; D010802:Phylogeny; D011110:Polymorphism, Genetic; D013045:Species Specificity; D014562:Caudata / Q000235:genetics*
D017931:DNA Primers