title: Depth-resolved structural imaging by third-harmonic generation microscopy.
abstract: Third harmonic generation microscopy is shown to be a robust method for obtaining structural information on a variety of biological specimens. Its nature allows depth-resolved imaging of inhomogeneities with virtually no background from surrounding homogeneous media. With an appropriate illumination geometry, third harmonic generation microscopy is shown to be particularly suitable for imaging of biogenic crystals, enabling extraction of the crystal orientation.
authors.affiliation: Department of Physics of Complex Systems, Weizmann Institute of Science, Rehovot IL-76100, Israel. dan.oron@weizmann.ac.il
authors.forename: Dan
authors.initials: D
authors.lastname: Oron
authors.forename: Dvir
authors.initials: D
authors.lastname: Yelin
authors.forename: Eran
authors.initials: E
authors.lastname: Tal
authors.forename: Sefi
authors.initials: S
authors.lastname: Raz
authors.forename: Rachel
authors.initials: R
authors.lastname: Fachima
authors.forename: Yaron
authors.initials: Y
authors.lastname: Silberberg
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000033:anatomy & histology; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D009865:Oocytes / Q000166:cytology; D001744:Urinary Bladder Calculi / Q000648:ultrastructure