A phage with high affinity for hepatitis B surface antigen for the detection of HBsAg.
An M13 phage, called PHH2, with the ability to bind HBsAg was isolated from a coat protein III display library. The region of the HBsAg polypeptide to which PHH2 binds was determined. The HBsAg binding phage was used in an assay referred to as "PHALISA", an abbreviation for phage-linked immune-absorbent assay. This assay was at least 20-100 times more sensitive in the detection of HBV antigen than conventional enzyme-linked immune-absorbent assays (ELISA). The application of this method for screening and detection of specific protein is discussed.
Lu
Xuanyong
X
Jefferson Center for Biomedical Research and Agricultural Medicine, Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, 700 East Butler Avenue, Doylestown, PA 18901, USA. xuanyong.lu@jefferson.edu
Weiss
Paul
P
Block
Timothy
T
D000595:Amino Acid Sequence; D017104:Bacteriophage M13 / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D019151:Peptide Library; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods / Q000706:statistics & numerical data
D017931:DNA Primers; D006514:Hepatitis B Surface Antigens; D019151:Peptide Library