title: Effect of two nitrogenous diphenyl ether pesticides on mast cell activation.
abstract: We examined the effect of two nitrogenous diphenyl ether pesticides, nitrofen (NIP) and chlornitrofen (CNP), on mast cell activation. RBL-2H3 (rat basophilic leukemia) cells were exposed to NIP or CNP for 30 min to investigate their effect on degranulation, and for 3 h to investigate their effect on cytokine production and gene expression. NIP and CNP increased IgE receptor-mediated beta-hexosaminidase release, MCP-1 release, and TNF-alpha release in a dose-dependent manner. The increasing effect of CNP on their release was greater than that of NIP. In the gene expression experiment, 30 microg/ml CNP significantly upregulated Egr-1, MCP-1 and GADD45a gene expression. These results suggest that at higher concentrations (more than 30 microg/ml) the nitrogenous diphenyl ether pesticides had both a degranulation-enhancing effect and proinflammatory cytokine-production enhancing effect through the expression of some transcription factors in RBL-2H3 cells.
authors.affiliation: Division of Biochemistry and Immunochemistry, National Institute of Health Sciences, Kamiyoga 1-18-1, Tokyo 158-8501, Japan. rteshima@nihs.go.jp
authors.forename: Reiko
authors.initials: R
authors.lastname: Teshima
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Nakamura
authors.forename: Osamu
authors.initials: O
authors.lastname: Nakajima
authors.forename: Akiko
authors.initials: A
authors.lastname: Hachisuka
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Sawada
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics; D015550:Cell Degranulation / Q000187:drug effects*; D045744:Cell Line, Tumor; D018932:Chemokine CCL2 / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D008407:Mast Cells / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D052066:N-Ethylmaleimide-Sensitive Proteins; D009687:Nuclear Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010575:Pesticides / Q000633:toxicity*; D010647:Phenyl Ethers / Q000633:toxicity*; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D015854:Up-Regulation; D033921:Vesicular Transport Proteins / Q000235:genetics; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism; D000097642:GADD45 Proteins
chemical_list: D018797:Cell Cycle Proteins; D018932:Chemokine CCL2; D052066:N-Ethylmaleimide-Sensitive Proteins; D009687:Nuclear Proteins; D010575:Pesticides; D010647:Phenyl Ethers; D014409:Tumor Necrosis Factor-alpha; D033921:Vesicular Transport Proteins; D001619:beta-N-Acetylhexosaminidases; C491766:Ccl2 protein, rat; C499073:Gadd45a protein, rat; D000097642:GADD45 Proteins; C010422:2,4,6-trichlorophenyl 4-nitrophenyl ether; C498737:Nsf protein, rat; C007350:nitrofen