Site-specific conjugation of oligonucleotides to the C-terminus of recombinant protein by expressed protein ligation.
We developed a convenient method for synthesizing homogeneous DNA-protein conjugates. The method is based on expressed protein ligation of intein-fusion proteins and oligonucleotides derivatized with a cysteine. A range of cysteinyl oligonucleotides were synthesized by using a new reagent 1 and were successfully applied to expressed protein ligation to attach the oligonucleotides specifically at the C-terminus of a recombinant protein.
Takeda
Shuji
S
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo 113-8656, Japan.
Tsukiji
Shinya
S
Nagamune
Teruyuki
T
D000595:Amino Acid Sequence; D003432:Cross-Linking Reagents; D003545:Cysteine / Q000737:chemistry; D047668:Inteins; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry*
D003432:Cross-Linking Reagents; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D003545:Cysteine