Cultured neurons coupled to microelectrode arrays: circuit models, simulations and experimental data.
The purpose of this paper is to characterize the neuron-microelectrode junction, based on the equivalent electric-circuit approach. As a result, recording of action potentials can be simulated with a general-purpose circuit simulation program such as HSPICE. The response of the microelectrode was analyzed as a function of parameters such as sealing resistance and adhesion conditions. The models of the neuron and microelectrode implemented in HSPICE were first described. These models were used to simulate the behavior of the junction between a patch of neuronal membrane (described by the compartmental model) and a microelectrode.
Martinoia
Sergio
S
Neuroengineering and Bio-nanoTechnology Group, Department of Biophysical and Electronic Engineering, University of Genova, Via Opera Pia 11a, 16145 Genova, Italy. giaser@dibe.unige.it
Massobrio
Paolo
P
Bove
Marco
M
Massobrio
Giuseppe
G
D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D003198:Computer Simulation; D004594:Electrophysiology / Q000379:methods*; D019544:Equipment Failure Analysis / Q000379:methods*; D008839:Microelectrodes; D008959:Models, Neurological; D009474:Neurons / Q000502:physiology*; D051381:Rats