title: Inhibitory effects of polyphenols in leaves of Artemisia princeps PAMP on protein fragmentation by Cu(II)-H2O2 in vitro.
abstract: The leaves of Artemisia princeps PAMP have traditionally been used as teas and foods in Japan. Polyphenols in Artemisia plants have been shown to have inhibitory effects against biological damages. The inhibitory effects of polyphenols in the leaves of A. princeps PAMP were investigated on protein fragmentation induced by Cu(II)-H(2)O(2) in vitro. The total polyphenol content in the leaves of A. princeps PAMP was 4.58%. The condensed tannin content was 0.62% by vanillin assay and 0.14% by proanthrocyanidin assay. The polyphenols in the leaves of A. princeps PAMP inhibited bovine albumin fragmentation by Cu(II)-H(2)O(2). The effects of polyphenols in the leaves of A. princeps PAMP were similar to those of tannic acid, studied as a related polyphenol. These results demonstrated that the leaves of A. princeps PAMP have inhibitory effects on protein fragmentation damage.
authors.affiliation: Department of Pharmaceutical Science, Kansai College of Oriental Medicine, Sen-nan, Osaka, Japan. toda@kansai.ac.jp
authors.forename: Shizuo
authors.initials: S
authors.lastname: Toda
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D019306:Artemisia / Q000737:chemistry; D003300:Copper / Q000037:antagonists & inhibitors / Q000633:toxicity*; D004591:Electrophoresis, Polyacrylamide Gel; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000633:toxicity*; D047348:Hydrolyzable Tannins / Q000032:analysis / Q000494:pharmacology; D010446:Peptide Fragments / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D010636:Phenols / Q000032:analysis / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D047348:Hydrolyzable Tannins; D010446:Peptide Fragments; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D012710:Serum Albumin, Bovine; D003300:Copper; D006861:Hydrogen Peroxide