Effect of coenzyme Q10 on the mitochondrial function of skin fibroblasts from Parkinson patients.
Several lines of evidence suggest an impairment of mitochondrial function in the brain of patients with Parkinson's disease (PD). However, the presence of a detectable mitochondrial defect in extracerebral tissue of these patients remains a matter of dispute. Therefore, we investigated mitochondrial function in fibroblasts of 18 PD patients applying biochemical micromethods. Putative beneficial effects of coenzyme Q(10) (CoQ(10)), a potent antioxidant, on the mitochondrial function of skin fibroblast cultures were evaluated. Applying inhibitor titrations of the mitochondrial respiration to calculate flux control coefficients of respiratory chain complexes I and IV, we observed deficiencies of both complexes in cultivated skin fibroblasts of PD patients. Cultivation of fibroblasts in the presence of 5 microM CoQ(10) restored the activity of impaired respiratory chain complexes in the fibroblast cultures of 9 out of 18 PD patients. Our data support the presence of a generalised mitochondrial defect in at least a subgroup of patients with PD that can be partially ameliorated in vitro by coenzyme Q(10) treatment.
Winkler-Stuck
Kirstin
K
Klinik für Neurologie der Otto-von-Guericke-Universität Magdeburg, Leipziger Strasse 44, 39120 Magdeburg, Germany.
Wiedemann
Falk R
FR
Wallesch
Claus-W
CW
Kunz
Wolfram S
WS
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D000368:Aged; D000654:Amobarbital / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D003067:Coenzymes; D004347:Drug Interactions; D005260:Female; D005347:Fibroblasts / Q000187:drug effects*; D018698:Glutamic Acid / Q000494:pharmacology; D006801:Humans; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology; D009243:NAD / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism; D010300:Parkinson Disease / Q000473:pathology*; D019289:Pyruvic Acid / Q000494:pharmacology; D012867:Skin / Q000473:pathology*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology*
D000975:Antioxidants; D003067:Coenzymes; D009243:NAD; D014451:Ubiquinone; D018698:Glutamic Acid; D000244:Adenosine Diphosphate; D019289:Pyruvic Acid; C024989:coenzyme Q10; D000654:Amobarbital; D010100:Oxygen