Analysis of gangliosides from carp intestinal mucosa.
The gangliosides of carp intestinal mucosa were isolated and analysed by thin-layer chromatography (TLC), TLC immunostaining test, and TLC/secondary ion mass spectrometry (TLC/SIMS). Four species of gangliosides, designated as G-1, G-2, G-3 and G-4, were separated on TLC. The TLC/SIMS analysis of the G-1 ganglioside of carp intestinal mucosa revealed a series of [M-H](-)ions from m/z 1061 to m/z 1131 representing the molecular mass range of GM4-like ganglioside with NeuAc. G-2, G-3 and G-4 gangliosides were analysed by the TLC immunostaining test. G-2 ganglioside was recognised by the monoclonal antibody specific for ganglioside GM1 (AGM-1 monoclonal antibody). However, G-3 ganglioside migrating on TLC between GM3 and GM1 ganglioside was not recognised by anti-GM3 monoclonal antibody and by AGM-1 monoclonal antibody. Furthermore, G-4 ganglioside with a similar TLC mobility as GD1a ganglioside did not show the reactivity to the anti-GD1a monoclonal antibody. In addition using the AGM-1 monoclonal antibody, the expression of GM1 ganglioside in the carp intestinal tissue was studied. GM1 ganglioside was detected on the epithelial cell surface of carp intestinal mucosa.
Irie
Takuya
T
Laboratory of Veterinary Immunology, Division of Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Gakuen-cho 1-1, Sakai, Osaka 599-8531, Japan.
Watarai
Shinobu
S
Kushi
Yasunori
Y
Kasama
Takeshi
T
Kodama
Hiroshi
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002347:Carps / Q000276:immunology / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D004847:Epithelial Cells / Q000276:immunology*; D019085:Fluorescent Antibody Technique, Direct; D005732:Gangliosides / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000276:immunology* / Q000378:metabolism; D018629:Spectrometry, Mass, Secondary Ion
D000911:Antibodies, Monoclonal; D005732:Gangliosides