[Ecdysone 20-monooxygenase activity of cytochrome P450 in plants and cell culture of Ajuga reptans L].
The concentration of cytochrome P450 and ecdysone 20-monooxygenase activity in plants and callus cell culture of the bugleweed Ajuga reptans L. were determined. The maximal ecdysone 20-monooxygenase activity of cytochrome P450 was found in vegetative rosettes of intact plants. During the stage of flowering, the ecdysone 20-monooxygenase activity of cytochrome P450 in plant leaves was higher than in other organs. It was demonstrated that the content of ecdysteroids in callus cell culture is higher than in the intact plant with concurrent retention of a high ecdysone-20-monooxygenase activity.
Alekseeva
L I
LI
Institute of Biology, Komi Scientific Center, Ural Division, Russian Academy of Sciences, Syktyvkar, 167982 Russia. alexeeva@ib.komisc.ru
D031328:Ajuga / Q000201:enzymology*; D001189:Aryl Hydrocarbon Hydroxylases / Q000737:chemistry / Q000378:metabolism*; D002478:Cells, Cultured; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D035263:Flowering Tops / Q000201:enzymology; D018515:Plant Leaves / Q000201:enzymology; D012621:Seasons; D013250:Steroid Hydroxylases / Q000737:chemistry / Q000378:metabolism*
D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; C020095:ecdysone 20-hydroxylase