title: Heritable transgene expression pattern imposed onto maize ubiquitin promoter by maize adh-1 matrix attachment regions: tissue and developmental specificity in maize transgenic plants.
abstract: Matrix attachment regions (MARs) have been used to enhance transgene expression and to reduce transgene expression instability in various organisms. In plants, contradictory data question the role of MAR sequences. To assess the use of MAR sequences in maize, we have used two well-characterized MARs from the maize adh-1 region. The MARs have been cloned either 5' to or at both sides of a reporter gene expression cassette to reconstitute a MAR-based domain. Histochemical staining revealed a new transgene expression pattern in roots of regenerated plants and their progeny. Furthermore, MARs systematically induced variegation. We show here that maize adh-1 MARs are able to modify transgene expression patterns as a heritable trait, giving a new and complementary outcome following use of MARs in genetic transformation.
authors.affiliation: UMR INRA-UBP 1095 Amélioration et Santé des Plantes, Domaine de Crouelle, 63039, Clermont-Ferrand, France.
authors.forename: François
authors.initials: F
authors.lastname: Torney
authors.forename: Anne
authors.initials: A
authors.lastname: Partier
authors.forename: Véronique
authors.initials: V
authors.lastname: Says-Lesage
authors.forename: Isabelle
authors.initials: I
authors.lastname: Nadaud
authors.forename: Pierre
authors.initials: P
authors.lastname: Barret
authors.forename: Michel
authors.initials: M
authors.lastname: Beckert
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000235:genetics*; D000080924:Aldehyde Dehydrogenase 1 Family; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D017931:DNA Primers; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics*; D045170:Matrix Attachment Regions / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D050697:Retinal Dehydrogenase; D025801:Ubiquitin / Q000235:genetics*; D003313:Zea mays / Q000235:genetics* / Q000254:growth & development
chemical_list: D017931:DNA Primers; D007527:Isoenzymes; D025801:Ubiquitin; D000080924:Aldehyde Dehydrogenase 1 Family; D000444:Aldehyde Dehydrogenase; D050697:Retinal Dehydrogenase; D005966:Glucuronidase