title: Fluconazole and itraconazole susceptibility of vaginal yeast isolates from Slovakia.
abstract: Vulvovaginal candidiasis is a common mucosal infection caused by opportunistic yeasts of the Candida genus. In this study, we isolated and identified the yeast species in the vagina of patients treated in the gynecology clinic and tested in vitro activities of fluconazole and itraconazole against 227 clinical yeast isolates by the NCCLS microdilution method. C. albicans (87.6%) was the most frequently identified species followed by C. glabrata (6.2%) and C. krusei (2.2%). Almost thirteen percent of yeast strains were resistant to fluconazole and 18.5% were resistant to itraconazole. Cross-resistance analyses of C. albicans isolates revealed that fluconazole resistance and itraconazole resistance were also associated with decreased susceptibilities to other azole derivatives mainly to ketoconazole and miconazole. At the same time no cross-resistance to polyene antibiotics amphotericin B and nystatin was observed. These results support the notion that antifungal agents used to treat vaginitis may be contributing to the drug resistance problem by promoting cross-resistance to a range of clinically used antifungals.
authors.affiliation: 1st Department of Obstetrics and Gynecology, Faculty of Medicine, Comenius University, Zochova 7, 811 03 Bratislava, Slovak Republic.
authors.forename: Monika
authors.initials: M
authors.lastname: Sojakova
authors.forename: Denisa
authors.initials: D
authors.lastname: Liptajova
authors.forename: Miroslav
authors.initials: M
authors.lastname: Borovsky
authors.forename: Julius
authors.initials: J
authors.lastname: Subik
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification*; D002176:Candida albicans / Q000187:drug effects / Q000302:isolation & purification; D041221:Candida glabrata / Q000187:drug effects / Q000302:isolation & purification; D002181:Candidiasis, Vulvovaginal / Q000382:microbiology; D025141:Drug Resistance, Fungal; D005260:Female; D015725:Fluconazole / Q000494:pharmacology*; D006801:Humans; D017964:Itraconazole / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D014621:Vagina / Q000382:microbiology*
chemical_list: D000935:Antifungal Agents; D017964:Itraconazole; D015725:Fluconazole