Studying vaccinia virus RNA processing in vitro.
The study and use of vaccinia virus-derived RNA-modifying proteins has made a significant contribution to molecular biology. Here, the purification and assay of two such proteins, comprising the vaccinia poly(A) polymerase/cap-specific mRNA 2'-O-methyltransferase, is described.
Gershon
Paul D
PD
Institute of Biosciences and Technology, Center for Genome Research, Texas A&M University Health Science Center, Houston, TX, USA.
D002460:Cell Line; D066298:In Vitro Techniques; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D014616:Vaccinia virus / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D012367:RNA, Viral; D009713:Nucleotidyltransferases