Liver fatty acid binding protein expression in colorectal neoplasia.
Liver fatty acid binding protein is a member of the fatty acid binding group of proteins that are involved in the intracellular transport of bioactive fatty acids and participate in intracellular signalling pathways, cell growth and differentiation. In this study we have used proteomics and immunohistochemistry to determine the changes in liver fatty acid binding protein in colorectal neoplasia. Comparative proteome analysis of paired samples colorectal cancer and normal colon identified consistent loss of liver fatty acid binding protein (L-FABP) in colorectal cancer compared with normal colon. To identify the changes in liver fatty acid binding protein expression during colorectal cancer development and progression the cell-specific expression of L-FABP was determined by immunohistochemistry in a series of colorectal cancers and colorectal adenomas. Decreased L-FABP immunoreactivity was significantly associated with poorly differentiated cancers (P<0.001). In colorectal adenomas there was a significant trend towards decreased staining of L-FABP in the larger adenomas (P<0.001). There was consistent L-FABP immunostaining of normal surface colonocytes. This study demonstrates that loss of L-FABP occurs at the adenoma stage of colorectal tumour development and also indicates that L-FABP is a marker of colorectal cancer differentiation.
Lawrie
L C
LC
Department of Pathology, University of Aberdeen, Foresterhill, Aberdeen, Foresterhill AB25 2ZD, UK.
Dundas
S R
SR
Curran
S
S
Murray
G I
GI
D000236:Adenoma / Q000235:genetics* / Q000473:pathology*; D000368:Aged; D000943:Antigens, Differentiation; D002352:Carrier Proteins / Q000096:biosynthesis*; D016022:Case-Control Studies; D002454:Cell Differentiation; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D018450:Disease Progression; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D040901:Proteomics; D015398:Signal Transduction; D025521:Tumor Suppressor Proteins
D000943:Antigens, Differentiation; D002352:Carrier Proteins; C493319:FABP1 protein, human; C107608:FABP7 protein, human; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D025521:Tumor Suppressor Proteins