title: Biocompatibility and biodegradation of cross-linked gelatin/hyaluronic acid sponge in rat subcutaneous tissue.
abstract: A gelatin/hyaluronic acid (GH) sponge has been fabricated by freeze-drying and cross-linking. The GH sponge was insoluble when cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide. The morphologies of sponges were investigated using a field emission scanning electron microscope. The porosity of the GH sponge increased with hyaluronic acid content. The GH sponge was biodegradable, as evidenced by implantation in Wistar rat subcutaneous connective tissue. Fibroblasts infiltrated into the sponge matrix, and regenerated collagen in the matrix to a level of 25% by 15 days after surgery. The GH73 sponge induced an acute inflammatory response compared with the GH91 sponge. This inflammatory response could have been stimulated by the presence of hyaluronic acid up to Day 10, as it decreased afterwards. The C-reactive protein of blood samples also indicated the same result. The blood tests and histological results show that GH sponges have good biocompatibility and low antigenicity for tissue engineering scaffolds.
authors.affiliation: School of Chemical Engineering, College of Engineering, Hanyang University, Seongdong-ku, Seoul, 133-791, South Korea.
authors.forename: Sung Ran
authors.initials: SR
authors.lastname: Hong
authors.forename: Moo Sang
authors.initials: MS
authors.lastname: Chong
authors.forename: Sang Bong
authors.initials: SB
authors.lastname: Lee
authors.forename: Young Moo
authors.initials: YM
authors.lastname: Lee
authors.forename: Kang Won
authors.initials: KW
authors.lastname: Song
authors.forename: Moon Hyang
authors.initials: MH
authors.lastname: Park
authors.forename: Sung Hwa
authors.initials: SH
authors.lastname: Hong
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D001673:Biodegradation, Environmental; D002097:C-Reactive Protein / Q000032:analysis*; D003432:Cross-Linking Reagents / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry* / Q000276:immunology / Q000633:toxicity; D006653:Histology; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000276:immunology / Q000633:toxicity; D007958:Leukocyte Count; D008855:Microscopy, Electron, Scanning; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects; D016062:Porosity; D019736:Prostheses and Implants; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D005780:Gelatin; D006820:Hyaluronic Acid; D002097:C-Reactive Protein