title: [Investigation of primary cell culture for dental pulp: comparing of coronal pulp and root pulp].
abstract: OBJECTIVE
  To compare and establish an ideal method for the culture of human primary dental coronal and root pulp cells.
  
  
  METHODS
  The whole dental pulp tissues were obtained from healthy and young human teeth extracted for orthodontic purposes. The cells in coronal and root pulp were separately cultured by the tissue explant and tissue explant-collagenase digestion(0.1% type I collagenase) methods. By evaluating the attachment efficiency,viability and the successfulness of the cultured cells, the optimal primary cell culture process of human dental coronal and root pulp was determined.
  
  
  RESULTS
  The tissue explant culture had more successfulness,more viability and less attachment efficiency than that of tissue explant-collagenase digestion method in the culture of human coronal and root dental pulp cells.
  
  
  CONCLUSIONS
  Tissue explant method was superior to tissue explant-collagenase digestion method for the culturing of human dental coronal and root pulp cells. The ideal method for dental coronal and root pulp cells was established in this study.
authors.affiliation: Oral and Maxillofacial Department, Qilu Hospital of Shangdong University,Jinan 250012, Shandong province, China. lshabccba@sdu.edu.cn
authors.forename: Shao-hua
authors.initials: SH
authors.lastname: Liu
authors.forename: Feng-cai
authors.initials: FC
authors.lastname: Wei
authors.forename: Shan-zhen
authors.initials: SZ
authors.lastname: Sun
authors.forename: Chun-yan
authors.initials: CY
authors.lastname: Zhang
authors.forename: Yun-sheng
authors.initials: YS
authors.lastname: Liu
mesh_terms: D000293:Adolescent; D000328:Adult; D018929:Cell Culture Techniques / Q000379:methods*; D003782:Dental Pulp / Q000166:cytology*; D006801:Humans