[Structural-functional characteristics of the 13q14 region of the human genome in the search for potential tumor suppressor genes].
Works on chromosome 13 mapping supported by the Russian program Human Genome are reviewed. Emphasis is placed on studies of region 13q14.3, which is often lost in some human tumors and potentially contains tumor suppressor genes (TSG). A strategy of TSG search is described. As the resolution of genome analysis improved, a minimal overlap of genetic loss in B-cell chronic lymphocytic leukemia (B-CLL) was established for chromosome 13. A map of expressed sequences was constructed for the region containing the overlap, and candidate TSG of chromosome 13q14 were identified. The candidate genes were analyzed both structurally and functionally, and their possible role in tumorigenesis was considered. Assuming haploinsufficiency as a genetic mechanism controlling B-CLL, a new strategy was proposed for mutation screening aimed at identifying potential TSG of region 13q14.
Baranova
A V
AV
Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991 Russia.
Ivanov
D V
DV
Tiazhelova
T V
TV
Iankovskiĭ
N K
NK
D002882:Chromosomes, Human, Pair 13; D016147:Genes, Tumor Suppressor; D015894:Genome, Human; D006801:Humans