Noninvasive auto-photoreduction used as a tool for studying structural changes in heme-copper oxidases by FTIR spectroscopy.
We demonstrate an efficient Fourier transform infrared (FTIR) spectroscopic method, termed "auto-photoreduction," that uses anaerobic photo-induced internal electron transfer to monitor reaction-initiated changes of heme-copper oxidases. It can be applied without the use of either expensive electrochemical equipment, or caged compounds, which cause significant background signals. At high irradiation power, carbon monoxide is released from high-spin heme a of cytochrome c oxidase and heme o from cytochrome bo(3). Photochemistry is initiated at wavelengths <355 nm, and the photochemical action spectrum has a maximum of 290 nm for cytochrome bo(3), which is consistent with the possible intermediate involvement of tyrosinate or an activated state of tyrosine. We propose that the final electron donors are proton channel water molecules. In the pH range of 4-9, the noninvasive auto-photoreduction method yields highly reproducible FTIR redox difference spectra within a broad range, resolving a number of vibrational changes outside the amide I region (1600-1640 cm(-1)). Furthermore, it provides details of redox-induced changes in the spectral region between 1600 and 1100 cm(-1). The auto-photoreduction method should be universally applicable to heme proteins.
Bettinger
Karin
K
Lehrstuhl für Biophysik, Ruhr-Universität Bochum, D-44780 Bochum, Germany.
Prutsch
Alexander
A
Vogtt
Karsten
K
Lübben
Mathias
M
D002248:Carbon Monoxide / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003573:Cytochrome b Group / Q000737:chemistry; D004563:Electrochemistry; D003576:Electron Transport Complex IV / Q000737:chemistry; D004583:Electrons; D004926:Escherichia coli / Q000378:metabolism; D006418:Heme / Q000737:chemistry*; D007834:Lasers; D008027:Light; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry*; D010777:Photochemistry; D011487:Protein Conformation; D012242:Rhodobacter sphaeroides / Q000378:metabolism; D013053:Spectrophotometry; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013997:Time Factors
D003573:Cytochrome b Group; C110232:cytochrome b(oa)3; C069808:heme O; D006418:Heme; D002248:Carbon Monoxide; D010088:Oxidoreductases; C024748:copper oxidase; D003576:Electron Transport Complex IV