Correlation of morphological findings with functional reserve in the aging donor: role of the poly (ADP-ribose) polymerase.
BACKGROUND
The enzyme poly (ADP-ribose) polymerase (PARP-1) participates in the first events of DNA repair in higher organisms. Under conditions of tissue ischemia, this action can lead to significant decreases in NAD(+), massive adenosine triphosphate (ATP) depletion, and cell death. In renal grafts with pretransplantation cold ischemia and subsequent ischemia-reperfusion injury, overactivation of PARP-1 may lead to a higher index of acute tubular necrosis, a delay in total recovery of the function of the transplanted organ, and an early progression to chronic graft nephropathy. The present study examined whether increased tubular expression of PARP-1 in kidneys from aged donors contributed to recipient renal function.


METHODS
We studied the nuclear expression of PARP-1 using immunohistochemistry with monoclonal antibody PAR01 in 75 kidney biopsy specimens from 40 aged donors.


RESULTS
Immunohistochemical expression of PARP-1 showed a statistically significant relationship with donor age (r =.408, P =.006, Spearman test), with time required to achieve effective diuresis (r =.386, P =.01, Spearman test) and with creatinine levels in the first 3 months. We also highlighted a greater intensity of PARP-1 expression in suboptimal donor kidneys that failed to reduce the serum creatinine levels to <1.7 mg/dL (creatinine <1.7 PARP: 1.29 +/- 1.49 vs creatinine >1.7 PARP: 2.29 +/- 1.33, P =.047, Mann-Whitney U test).


CONCLUSIONS
We conclude that the determination of PARP-1 in biopsy specimens from aged donors may be a useful predictive factor for renal graft function.
O'Valle
F
F
Department of Pathology, San Cecilio University Hospital, Granada, Spain.
Benitez
M C
MC
Gómez-Morales
M
M
Bravo
J
J
Osuna
A
A
del Moral
R M G
RM
Martin-Oliva
D
D
Oliver
F J
FJ
del Moral
R G
RG
D000368:Aged; D001331:Automation; D001706:Biopsy; D006801:Humans; D007150:Immunohistochemistry; D007677:Kidney Function Tests; D016030:Kidney Transplantation / Q000473:pathology / Q000502:physiology*; D011065:Poly(ADP-ribose) Polymerases / Q000032:analysis* / Q000235:genetics; D011237:Predictive Value of Tests; D014019:Tissue Donors
D011065:Poly(ADP-ribose) Polymerases