Effects of Bt plants on the development and survival of the parasitoid Cotesia plutellae (Hymenoptera: Braconidae) in susceptible and Bt-resistant larvae of the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae).
A range of crops have been transformed with delta-endotoxin genes from Bacillus thuringiensis (Bt) to produce transgenic plants with high levels of resistance to lepidopteran pests. Parasitoids are important natural enemies of lepidopteran larvae and the effects of Bt plants on these non-target insects have to be investigated to avoid unnecessary disruption of biological control. This study investigated the effects of Cry1Ac-expressing transgenic oilseed rape (Brassica napus) on the solitary braconid endoparasitoid Cotesia plutellae in small-scale laboratory experiments. C. plutellae is an important natural enemy of the diamondback moth (Plutella xylostella), the most important pest of brassica crops world-wide. Bt oilseed rape caused 100% mortality of a Bt-susceptible P. xylostella strain but no mortality of the Bt-resistant P. xylostella strain NO-QA. C. plutellae eggs laid in Bt-susceptible hosts feeding on Bt leaves hatched but premature host mortality did not allow C. plutellae larvae to complete their development. In contrast, C. plutellae developed to maturity in Bt-resistant hosts fed on Bt oilseed rape leaves and there was no effect of Bt plants on percentage parasitism, time to emergence from hosts, time to adult emergence and percentage adult emergence from cocoons. Weights of female progeny after development in Bt-resistant hosts did not differ between plant types but male progeny was significantly heavier on wildtype plants in one of two experiments. The proportion of female progeny was significantly higher on Bt plants in the first experiment with Bt-resistant hosts but this effect was not observed again when the experiment was repeated.
Schuler
Tanja H
TH
Division of Plant and Invertebrate Ecology, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK. tanja.schuler@bbsrc.ac.uk
Denholm
Ian
I
Clark
Suzanne J
SJ
Stewart
C Neal
CN
Poppy
Guy M
GM
D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics / Q000494:pharmacology; D001835:Body Weight; D029741:Brassica rapa / Q000235:genetics / Q000469:parasitology*; D018556:Crops, Agricultural / Q000235:genetics / Q000469:parasitology; D005260:Female; D006790:Host-Parasite Interactions; D006927:Hymenoptera / Q000187:drug effects / Q000502:physiology*; D007305:Insecticide Resistance; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D008297:Male; D008675:Metamorphosis, Biological; D009036:Moths / Q000187:drug effects / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000469:parasitology*; D011679:Pupa / Q000187:drug effects / Q000254:growth & development; D012744:Sex Ratio; D013997:Time Factors
D001427:Bacterial Toxins