title: Feasibility of polysaccharide hybrid materials for scaffolds in cartilage tissue engineering: evaluation of chondrocyte adhesion to polyion complex fibers prepared from alginate and chitosan.
abstract: The ideal cell-carrier material for cartilage regeneration should be one that closely mimics the natural environment in a living articular cartilage matrix. In the current study, we considered that alginate-based chitosan hybrid biomaterials could provide excellent supports for chondrocyte adhesion. To test this hypothesis, we investigated the adhesion behavior of rabbit chondrocytes onto an alginate polymer versus the adhesion of the chondrocytes onto some alginate-based chitosan hybrid polymer fibers in vitro. We demonstrated that the alginate-based chitosan hybrid polymer fibers showed much improved adhesion capacity with chondrocytes in comparison with alginate polymer fiber. Additionally, morphologic studies revealed maintenance of the characteristic round morphology of the chondrocyte and the dense fiber of the type II collagen produced by the chondrocytes in the hybrid polymer. On the basis of these results, we conclude that an alginate-based chitosan hybrid polymer fiber has considerable potential as a desirable biomaterial for cartilage tissue scaffolds.
authors.affiliation: Department of Orthopaedic Surgery, Hokkaido University School of Medicine, Sapporo 060-8638, Japan. niwasaki@med.hokudai.ac.jp
authors.forename: Norimasa
authors.initials: N
authors.lastname: Iwasaki
authors.forename: Shin-Tarou
authors.initials: ST
authors.lastname: Yamane
authors.forename: Tokifumi
authors.initials: T
authors.lastname: Majima
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Kasahara
authors.forename: Akio
authors.initials: A
authors.lastname: Minami
authors.forename: Kazuo
authors.initials: K
authors.lastname: Harada
authors.forename: Sachiko
authors.initials: S
authors.lastname: Nonaka
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Maekawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tamura
authors.forename: Seiichi
authors.initials: S
authors.lastname: Tokura
authors.forename: Masamichi
authors.initials: M
authors.lastname: Shiono
authors.forename: Kenji
authors.initials: K
authors.lastname: Monde
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Nishimura
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology*; D002448:Cell Adhesion; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry*; D019902:Chondrocytes / Q000166:cytology*; D004247:DNA / Q000032:analysis; D005240:Feasibility Studies; D020723:Glucuronic Acid / Q000737:chemistry*; D006603:Hexuronic Acids / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D011134:Polysaccharides / Q000737:chemistry*; D011817:Rabbits; D023822:Tissue Engineering
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D011134:Polysaccharides; D020723:Glucuronic Acid; D004247:DNA; D048271:Chitosan