[Induction of IgG and IgM production by Epstein-Barr virus protein].
OBJECTIVE
To detect the production of IgG and IgM by cultured umbilical blood B cell stimulated by UV and heat-inactivated Epstein-Barr virus (EBV).


METHODS
Mononuclear cells were isolated routinely from umbilical blood, then monocytes, NK cells and cytotoxic T cells were eliminated by L-leucine methy ester method; remained T cells were eliminated by AET-SRBC rosette method. The purified B cells were treated with UV or heat-inactivated EBV respectively and cultured in complete IMDM. The IgG and IgM in the supernatant were detected by ELISA.


RESULTS
The IgG and IgM production(A value) in UV-inactivated EBV group increased from 18th day to 30 th day after stimulation compared with the baseline. The A value of all time points in heat-inactivated EBV group had no significant difference.


CONCLUSIONS
UV-inactivated EBV may induce IgG and IgM production with a time-effect relationship, suggesting that EBV protein is a key component of stimulating Ig production. This result provide the basis for the further researching the effects of functional EBV protein on the production of natural autoantibodies (NAA).
Zhang
Yan-guo
YG
Center of Dermatology of Chinese PLA, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Fu
Meng
M
Liu
Yu-fengg
YF
Gao
Tian-wen
TW
Pan
Lei
L
Wang
Lin
L
D001402:B-Lymphocytes / Q000276:immunology / Q000821:virology; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology; D004854:Herpesvirus 4, Human / Q000528:radiation effects; D006358:Hot Temperature; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000378:metabolism*
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014764:Viral Proteins