Increased adrenomedullin expression in lungs in endotoxaemia.
Adrenomedullin (AM) is a peptide involved in cardiovascular homeostasis and in inflammation. We examined its expression in a rat model of endotoxaemia. Male Sprague-Dawley rats received intraperitoneal injection of 5 or 10 mg/kg lipopolysaccharide (LPS), or saline as control. Rats were killed at 1, 3, 6, 12 and 24 h after injection. LPS at 5 mg/kg, but not saline, increased plasma AM significantly at 3 h. At 10 mg/kg, plasma AM was raised at 3, 6 and 12 h. Immunoreactive AM concentration in lung increased after 5 or 10 mg/kg LPS, but not saline. PreproAM mRNA level in lung was significantly increased at 3 and 6 h. In conclusion, endotoxin stimulates the expression of AM in the lungs and increases its circulatory concentration. AM may be involved in the systemic response to sepsis.
Cheung
B M Y
BM
Department of Medicine, University of Hong Kong, Hong Kong, China. mycheung@hkucc.hku.hk
Hwang
I S S
IS
Li
C Y Y
CY
O
W-S
WS
Tsang
K W T
KW
Leung
R Y H
RY
Kumana
C R
CR
Tang
F
F
D053607:Adrenomedullin; D000818:Animals; D019446:Endotoxemia / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D008070:Lipopolysaccharides; D008168:Lung / Q000737:chemistry*; D008297:Male; D023421:Models, Animal; D010455:Peptides / Q000032:analysis* / Q000097:blood; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D008070:Lipopolysaccharides; D010455:Peptides; D011498:Protein Precursors; D012333:RNA, Messenger; C112034:preproadrenomedullin; D053607:Adrenomedullin