title: Specific PCR assay for a tannin-tolerant selenomonas ruminantium isolate, derived from helicase coding sequences.
abstract: Sequences from a tannin-tolerant Selenomonas ruminantium isolate (EAT2) that hydrolyzes gallic acid were identified. Two exhibited identity to helicases with a wide phylogenetic distribution. PCR amplification by using primers from one helicase gene detected 2000 to 5000 EAT2 genome equivalents but did not amplify total gastrointestinal microbial DNA of nine other ungulate species.
authors.affiliation: The International Livestock Research Institute, Nairobi, Kenya, and Addis Ababa, Ethiopia. R.Bishop@cgiar.org
authors.forename: Richard
authors.initials: R
authors.lastname: Bishop
authors.forename: Moses
authors.initials: M
authors.lastname: Obura
authors.forename: David
authors.initials: D
authors.lastname: Odongo
authors.forename: Agnes
authors.initials: A
authors.lastname: Odenyo
mesh_terms: D000818:Animals; D000835:Animals, Wild; D004265:DNA Helicases / Q000235:genetics* / Q000378:metabolism; D005707:Gallic Acid / Q000378:metabolism*; D006041:Goats; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012417:Rumen / Q000382:microbiology; D012418:Ruminants / Q000378:metabolism / Q000382:microbiology*; D020357:Selenomonas / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012756:Sheep; D013045:Species Specificity; D013634:Tannins / Q000378:metabolism*
chemical_list: D013634:Tannins; D005707:Gallic Acid; D004265:DNA Helicases