Theoretical analysis of twist/bend ratio and mechanical moduli of bacterial flagellar hook and filament.
Certain motile bacteria employ rotating flagella for propulsion. The relative flexibility of two key components of the flagellum, filament and hook, is partially responsible for the mechanistic workings of this motor. A new computational method, the quantized elastic deformational model, was employed in this article to calculate the dimensionless twist/bend ratio (EI/GJ) of the filament and hook, providing a quantitative means to compare their relative stiffness. Both ratios were much <1.0, an average of 0.0440 for the filament and 0.0512 for the hook, indicating that within each structure bending is favored over twisting. These two ratios, along with previous experimental measurements, allowed us to propose a theoretical Young's modulus (E) between 10(6) and 10(7) dyn/cm(2) for the hook. This value is orders of magnitude smaller than experimentally determined Young's moduli of the filament, hence in agreement with empirical evidence linking compliance in the flagellum mainly to the hook.
Flynn
Terence C
TC
Department of Bioengineering, Rice University, Houston, Texas 77005, USA.
Ma
Jianpeng
J
D000818:Animals; D001419:Bacteria / Q000648:ultrastructure; D018407:Bacterial Physiological Phenomena; D001426:Bacterial Proteins / Q000737:chemistry; D001696:Biomechanical Phenomena; D001703:Biophysics / Q000379:methods*; D005407:Flagella / Q000737:chemistry* / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D008962:Models, Theoretical; D009068:Movement; D012486:Salmonella typhimurium / Q000502:physiology
D001426:Bacterial Proteins