title: Processing of plasmid DNA with ColE1-like replication origin.
abstract: With the increasing utilization of plasmid DNA as a biopharmaceutical drug, there is a rapidly growing need for high quality plasmid DNA for drug applications. Although there are several different kinds of replication origins, ColE1-like replication origin is the most extensively used origin in biotechnology. This review addresses problems in upstream and downstream processing of plasmid DNA with ColE1-like origin as drug applications. In upstream processing of plasmid DNA, regulation of replication of ColE1-like origin was discussed. In downstream processing of plasmid DNA, we analyzed simple, robust, and scalable methods, which can be used in the efficient production of pharmaceutical-grade plasmid DNA.
authors.affiliation: Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, 200032 Shanghai, People's Republic of China. zjwang@shmu.edu.cn
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Wang
authors.forename: Zhenghong
authors.initials: Z
authors.lastname: Yuan
authors.forename: Ulrich R
authors.initials: UR
authors.lastname: Hengge
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D004261:DNA Replication / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D008957:Models, Genetic; D010957:Plasmids / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics; D018741:Replication Origin / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; C042833:ROM protein, Bacteria; D012343:RNA, Transfer