title: In vivo tracking of T cell development, ablation, and engraftment in transgenic zebrafish.
abstract: Transgenic zebrafish that express GFP under control of the T cell-specific tyrosine kinase (lck) promoter were used to analyze critical aspects of the immune system, including patterns of T cell development and T cell homing after transplant. GFP-labeled T cells could be ablated in larvae by either irradiation or dexamethasone added to the water, illustrating that T cells have evolutionarily conserved responses to chemical and radiation ablation. In transplant experiments, thymocytes from lck-GFP fish repopulated the thymus of irradiated wild-type fish only transiently, suggesting that the thymus contains only short-term thymic repopulating cells. By contrast, whole kidney marrow permanently reconstituted the T lymphoid compartment of irradiated wild-type fish, suggesting that long-term thymic repopulating cells reside in the kidney.
authors.affiliation: Pediatric Oncology, Dana-Farber Cancer Institute, Boston, MA 02115.
authors.forename: David M
authors.initials: DM
authors.lastname: Langenau
authors.forename: Adolfo A
authors.initials: AA
authors.lastname: Ferrando
authors.forename: David
authors.initials: D
authors.lastname: Traver
authors.forename: Jeffery L
authors.initials: JL
authors.lastname: Kutok
authors.forename: John-Paul D
authors.initials: JP
authors.lastname: Hezel
authors.forename: John P
authors.initials: JP
authors.lastname: Kanki
authors.forename: Leonard I
authors.initials: LI
authors.lastname: Zon
authors.forename: A Thomas
authors.initials: AT
authors.lastname: Look
authors.forename: Nikolaus S
authors.initials: NS
authors.lastname: Trede
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000276:immunology*; D003907:Dexamethasone / Q000494:pharmacology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D015027:Zebrafish / Q000276:immunology*
chemical_list: D003907:Dexamethasone