title: Structure of N-glycosidic carbohydrates of secretory proteins of Tetrahymena thermophila.
abstract: Glycoproteins secreted by Tetrahymena into the culture medium were isolated and the N-glycosidic oligosaccharides analyzed using lectin blots and fluorophore-assisted carbohydrate gel electrophoresis (FACE). Lectin blots showed that the glycoproteins secreted by Tetrahymena contain only N-glycosidic structures of the high mannose type. Further analysis using the FACE technology revealed the presence of four different N-glycosidic structures differing only in the number of mannose residues attached to the core chitobiose unit.
authors.affiliation: Botanisches Institut, Universität zu Köln, Gyrhofstr. 15, 50931 Köln, Germany. b.becker@uni-koeln.de
authors.forename: Burkhard
authors.initials: B
authors.lastname: Becker
authors.forename: Matthias
authors.initials: M
authors.lastname: Rüsing
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D018929:Cell Culture Techniques; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D016808:Tetrahymena thermophila / Q000737:chemistry*
chemical_list: D006023:Glycoproteins; D009844:Oligosaccharides