A novel role of Mgm1p, a dynamin-related GTPase, in ATP synthase assembly and cristae formation/maintenance.
In Saccharomyces cerevisiae, two mitochondrial inner-membrane proteins play critical roles in organellar morphology. One is a dynamin-related GTPase, Mgm1p, which participates in mitochondrial fusion. Another is Tim11p, which is required for oligomeric assembly of F1Fo-ATP synthase, which generates ATP through oxidative phosphorylation. Our data bring these findings together and define a novel role for Mgm1p in the formation and maintenance of mitochondrial cristae. We show that Mgm1p serves as an upstream regulator of Tim11p protein stability, ATP synthase assembly, cristae morphology and cytochrome c storage within cristae.
Amutha
Boominathan
B
Department of Pharmacology and Physiology, UMDNJ-New Jersey Medical School, 185 South Orange Avenue, MSB I-669, Newark, NJ 07101-1709, U.S.A.
Gordon
Donna M
DM
Gu
Yajuan
Y
Pain
Debkumar
D
D045304:Cytochromes c / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D008854:Microscopy, Electron / Q000379:methods; D008928:Mitochondria / Q000737:chemistry* / Q000201:enzymology / Q000648:ultrastructure; D024101:Mitochondrial Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D025261:Mitochondrial Proton-Translocating ATPases / Q000737:chemistry / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D009687:Nuclear Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D015388:Organelles / Q000737:chemistry / Q000201:enzymology / Q000648:ultrastructure; D010641:Phenotype; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000201:enzymology / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012697:Serine Endopeptidases
C014059:CYC1 protein, S cerevisiae; C082883:MGM1 protein, S cerevisiae; D024101:Mitochondrial Proteins; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D045304:Cytochromes c; C490256:Pcp1 protein, S cerevisiae; D012697:Serine Endopeptidases; D019204:GTP-Binding Proteins; D025261:Mitochondrial Proton-Translocating ATPases; C102550:TIM11 protein, S cerevisiae; D006180:Proton-Translocating ATPases