title: Mechanism of 2-aminopurine-stimulated mutagenesis in Escherichia coli.
abstract: 2-Aminopurine (2AP), a base analog, causes both transition and frameshift mutations in Escherichia coli. The analog is thought to cause mutations by two mechanisms: directly, by mispairing with cytosine, and indirectly, by saturation of mismatch repair (MMR). The goal of this work was to measure the relative contribution of these two mechanisms to the occurrence of transition mutations. Our data suggest that, in contrast to 2-aminopurine-stimulated frameshift mutations, the majority of transition mutations are a direct effect of base mispairing.
authors.affiliation: Department of Biology, Concordia University, Montréal, Quebec, Canada.
authors.forename: Photini
authors.initials: P
authors.lastname: Pitsikas
authors.forename: Jason M
authors.initials: JM
authors.lastname: Patapas
authors.forename: Claire G
authors.initials: CG
authors.lastname: Cupples
mesh_terms: D015075:2-Aminopurine; D001665:Binding Sites; D003596:Cytosine / Q000737:chemistry; D004260:DNA Repair; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000235:genetics*; D008954:Models, Biological; D016296:Mutagenesis; D009153:Mutagens; D009154:Mutation; D010957:Plasmids / Q000378:metabolism
chemical_list: D009153:Mutagens; D015075:2-Aminopurine; D003596:Cytosine