title: [Protective effect of ONO-1078, a leukotriene receptor antagonist, on focal cerebral ischemia induced by endothelin-1 in rats].
abstract: OBJECTIVE
  To determine the protective effect of ONO-1078, a leukotriene receptor antagonist, on focal cerebral ischemia induced by endothelin-1 in rats.
  
  
  METHODS
  Slow microinjection of endothelin-1 (120 pmol in 6 microL, for > 6 min) into the region near the middle cerebral artery was used to induce focal cerebral ischemia. ONO-1078 (0.1 mg.kg-1) was i.p. injected 1 h before endothelin-1 injection. Neurological symptoms, brain edema, brain infarction size, and the survival neurons in cortex and striatum were observed 24 h after ischemia.
  
  
  RESULTS
  Intracerebral microinjection of endothelin-1 induced remarkable neurological symptoms, brain infarction, brain edema, and decrease of survival neurons in the cortex and striatum. In rats pretreated with ONO-1078, endothelin-1-induced brain edema and brain infarction size were decreased. The numbers of survival neurons in striatum and cortex were increased significantly. The neurological symptoms were improved but not significantly.
  
  
  CONCLUSIONS
  ONO-1078 possesses neuroprotective effect against cerebral ischemic injury induced by endothelin-1, therefore, leukotrienes may play a role in the injury of cerebral ischemia.
authors.affiliation: Department of Pharmacology, Medical School, Zhejiang University, Hangzhou 310031, China.
authors.forename: Shi-hong
authors.initials: SH
authors.lastname: Zhang
authors.forename: Er-qing
authors.initials: EQ
authors.lastname: Wei
authors.forename: Chao-yang
authors.initials: CY
authors.lastname: Zhu
authors.forename: Zhong
authors.initials: Z
authors.lastname: Chen
authors.forename: Song-fa
authors.initials: SF
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001929:Brain Edema / Q000473:pathology; D002545:Brain Ischemia / Q000139:chemically induced / Q000473:pathology*; D002540:Cerebral Cortex / Q000473:pathology; D002544:Cerebral Infarction / Q000473:pathology; D002867:Chromones / Q000494:pharmacology*; D003342:Corpus Striatum / Q000473:pathology; D019332:Endothelin-1; D020024:Leukotriene Antagonists / Q000494:pharmacology*; D008297:Male; D009474:Neurons / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002867:Chromones; D019332:Endothelin-1; D020024:Leukotriene Antagonists; D018696:Neuroprotective Agents; C047681:pranlukast