In vitro homocysteine inhibits platelet Na+,K+-ATPase and serum butyrylcholinesterase activities of young rats.
Homocystinuria is an inborn error of sulphur amino acid metabolism, resulting in accumulation of tissue homocysteine. This disease is characterized predominantly by vascular and nervous system dysfunction. In the present study we investigated the in vitro effects of homocysteine, the main metabolite accumulated in homocystinuria, on platelet Na+,K+-ATPase and serum butyrylcholinesterase (BuChE) activities of young rats. Platelet and serum of 29-day-old Wistar rats were incubated in the absence (control) or presence of homocysteine (0.01-0.5 mM). Results showed that Na+,K+-ATPase and BuChE activities were significantly inhibited by homocysteine. It is proposed that inhibition of Na+,K+-ATPase and BuChE activities might be one useful peripheral marker for the neurotoxic effects of homocysteine.
Stefanello
Francieli M
FM
Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, Porto Alegre, RS, Brazil.
Franzon
Renata
R
Wannmacher
Clovis M D
CM
Wajner
Moacir
M
Wyse
Angela T S
AT
D000818:Animals; D001792:Blood Platelets / Q000201:enzymology*; D002091:Butyrylcholinesterase / Q000097:blood / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006710:Homocysteine / Q000008:administration & dosage / Q000494:pharmacology*; D066298:In Vitro Techniques; D009994:Osmolar Concentration; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors* / Q000097:blood
D006710:Homocysteine; D002091:Butyrylcholinesterase; D000254:Sodium-Potassium-Exchanging ATPase