title: Antimicrobial and antioxidant activities of Melissa officinalis L. (Lamiaceae) essential oil.
abstract: The present study describes antimicrobial and free radical scavenging capacity (RSC) together with the effects on lipid peroxidation (LP) of Melissa officinalis essential oil. The chemical profile of essential oil was evaluated by the means of gas chromatography-mass spectrometry (GC-MS) and thin-layer chromatography (TLC). RSC was assessed measuring the scavenging activity of essential oil on the 2,2-diphenyl-1-picrylhydrazyl (DPPH(*)) and OH(*) radicals. The effect on LP was evaluated following the activities on Fe(2+)/ascorbate and Fe(2+)/H(2)O(2) systems of induction. The antimicrobial activity was tested against 13 bacterial strains and six fungi. The examined essential oil exhibited very strong RSC, reducing the DPPH radical formation (IC(50) = 7.58 microg/mL) and OH radical generation (IC(50) = 1.74 microg/mL) in a dose-dependent manner. According to the GC-MS and TLC (dot-blot techniques), the most powerful scavenging compounds were monoterpene aldehydes and ketones (neral/geranial, citronellal, isomenthone, and menthone) and mono- and sesquiterpene hydrocarbons (E-caryophyllene). Very strong inhibition of LP, particularly in the Fe(2+)/H(2)O(2) system of induction (94.59% for 2.13 microg/mL), was observed in both cases, also in a dose-dependent manner. The most effective antibacterial activity was expressed on a multiresistant strain of Shigella sonei. A significant rate of antifungal activity was exhibited on Trichophyton species.
authors.affiliation: Department of Chemistry, Faculty of Natural Sciences, Trg D. Obradovica 3, University of Novi Sad, 21000 Novi Sad, Serbia and Montenegro. mimica@ih.ns.ac.yu
authors.forename: Neda
authors.initials: N
authors.lastname: Mimica-Dukic
authors.forename: Biljana
authors.initials: B
authors.lastname: Bozin
authors.forename: Marina
authors.initials: M
authors.lastname: Sokovic
authors.forename: Natasa
authors.initials: N
authors.lastname: Simin
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis* / Q000494:pharmacology; D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002855:Chromatography, Thin Layer; D016166:Free Radical Scavengers / Q000494:pharmacology; D005658:Fungi / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D015227:Lipid Peroxidation / Q000187:drug effects; D031334:Melissa / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D009822:Oils, Volatile; D010938:Plant Oils