Specific detection and identification of herpes B virus by a PCR-microplate hybridization assay.
Herpes B virus DNA was specifically amplified by PCR, targeting the regions that did not cross-react with herpes simplex virus (HSV). The amplified products, which were shown to be highly genetic polymorphisms among herpes B virus isolates, were identified by microplate hybridization with probes generated by PCR. The products immobilized in microplate wells were hybridized with the biotin-labeled probes derived from the SMHV strain of herpes B virus. The amplified products derived from the SMHV and E2490 strains of herpes B virus were identified by microplate hybridization. PCR products amplified from the trigeminal ganglia of seropositive cynomolgus macaques were identified as herpes B virus DNA. The utility of the PCR-microplate hybridization assay for genetic detection and identification of the polymorphic region of herpes B virus was determined.
Oya
Chika
C
Department of Veterinary Public Health, Nippon Veterinary and Animal Science University, Musashino, Tokyo 180-8602, Japan.
Ochiai
Yoshitsugu
Y
Taniuchi
Yojiro
Y
Takano
Takashi
T
Ueda
Fukiko
F
Yoshikawa
Yasuhiro
Y
Hondo
Ryo
R
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D006566:Herpesviridae Infections / Q000821:virology; D006568:Herpesvirus 1, Cercopithecine / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008252:Macaca fascicularis; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D012668:Trigeminal Ganglion / Q000821:virology
D017931:DNA Primers; D004279:DNA, Viral