title: The auxotrophic aroA mutant of Aeromonas hydrophila as a live attenuated vaccine against A. salmonicida infections in rainbow trout (Oncorhynchus mykiss).
abstract: An auxotrophic aroA mutant of the Aeromonas hydrophila AG2 strain is a live attenuated vaccine against A. hydrophila infection in rainbow trout (Oncorhynchus mykiss). The protection conferred by the live attenuated vaccine against A. salmonicida strains is reported here, and several parameters of the specific and non-specific immune response in vaccinated trout were characterised. Vaccination with a dose of 10(7)cells/fish of the aroA mutant elicited significant protection against the Hooke and DK30 strains of A. salmonicida (relative percent survival RPS >60%). This cross-protection correlated moderately with the activation of the humoral and cellular specific immune responses, which show cross-reactivity against antigens shared by the two bacterial species, and a moderate increase in the lysozyme and antiprotease activities in the serum of vaccinated trout.
authors.affiliation: Departmento de Biología Celular, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, 24071 León, Spain.
authors.forename: Jose
authors.initials: J
authors.lastname: Vivas
authors.forename: Jorge
authors.initials: J
authors.lastname: Riaño
authors.forename: Begoña
authors.initials: B
authors.lastname: Carracedo
authors.forename: Blanca E
authors.initials: BE
authors.lastname: Razquin
authors.forename: Pilar
authors.initials: P
authors.lastname: López-Fierro
authors.forename: Germán
authors.initials: G
authors.lastname: Naharro
authors.forename: Alberto J
authors.initials: AJ
authors.lastname: Villena
mesh_terms: D000333:Aeromonas / Q000276:immunology*; D000372:Agglutination Tests; D000704:Analysis of Variance; D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000517:prevention & control / Q000662:veterinary*; D007964:Leukocytosis / Q000276:immunology; D009113:Muramidase / Q000276:immunology / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000382:microbiology*; D011480:Protease Inhibitors / Q000276:immunology / Q000378:metabolism; D014613:Vaccines, Attenuated / Q000276:immunology
chemical_list: D001428:Bacterial Vaccines; D011480:Protease Inhibitors; D014613:Vaccines, Attenuated; D009113:Muramidase