title: Molecular cytogentic profiles of hepatitis C infection in patients at Sharkia Governorate, Egypt.
abstract: It has become apparent that hepatitis C virus (HCV) infection is a major risk factor for the development of hepatocellular carcinoma (HCC) worldwide. The precise mechanism by which HCV causes HCC is not known. Unlike the hepatitis B virus (HBV), HCV is not a DNA virus and does not become integrated within the genome of hepatocytes. It is more likely that HCC occurs against a background of inflammation and regeneration, associated with liver injury due to chronic hepatitis. In this study, 40 of paraffin blocks liver tissues from HCV-PCR positive patients (HBV seronegative) were examined using DNA image cytometry to evaluate its role in diagnosing HCC associated with HCV infection. Fluorescent in situ hybridization (FISH) technique using LSIZNF 217 chromosome 20q 13.2 probe was applied as well. The results showed high percentage of S-phase fraction in cases of G2S2 and G3S3 with DNA diploidy. Only two cases of G3S3 showed DNA aneuploidy with severe amplification of chromosome 20q 13.2. Consequently, DNA imaging cytometry is a good approach in differentiating dysplasia from well-differentiated HCC on top of HCV infection. In conclusion HCV has an acquired role in development of HCC through amplification of the aggressive tumor behavior oncogene LSIZNF 217 at chromosome 20q 13.2.
authors.affiliation: Faculty of Medicine, Ain Shams University, Cairo, Egypt.
authors.forename: Ezzat
authors.initials: E
authors.lastname: el-Sobky
authors.forename: Amal M
authors.initials: AM
authors.lastname: Mangoud
authors.forename: Seham
authors.initials: S
authors.lastname: Mahrous
authors.forename: Mostafa H
authors.initials: MH
authors.lastname: Eissa
authors.forename: Essam I
authors.initials: EI
authors.lastname: Sabee
authors.forename: Ibrahem A
authors.initials: IA
authors.lastname: Ibrahem
authors.forename: Alaa
authors.initials: A
authors.lastname: Ismail
authors.forename: Tosson A
authors.initials: TA
authors.lastname: Morsy
authors.forename: Essam
authors.initials: E
authors.lastname: Nor Edin
authors.forename: Ismail
authors.initials: I
authors.lastname: el-Naggar
authors.forename: Yousry
authors.initials: Y
authors.lastname: Mostafa
authors.forename: Yousry
authors.initials: Y
authors.lastname: Abuel-Magd
authors.forename: Afefy F
authors.initials: AF
authors.lastname: Afefy
authors.forename: Eman
authors.initials: E
authors.lastname: el-Shorbagy
authors.forename: Mahmoud
authors.initials: M
authors.lastname: el-Sadawy
authors.forename: Hosnia
authors.initials: H
authors.lastname: Ragab
authors.forename: Ahmed
authors.initials: A
authors.lastname: el-Far
authors.forename: Mostafa I
authors.initials: MI
authors.lastname: Hassan
authors.forename: Khalid
authors.initials: K
authors.lastname: Lakouz
authors.forename: Khalid
authors.initials: K
authors.lastname: Abdel-Aziz
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Saber
authors.forename: Gaber
authors.initials: G
authors.lastname: el-Hady
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis; D004534:Egypt; D005260:Female; D005434:Flow Cytometry; D016679:Genome, Viral; D016174:Hepacivirus / Q000235:genetics; D019698:Hepatitis C, Chronic / Q000150:complications / Q000235:genetics* / Q000473:pathology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008099:Liver / Q000473:pathology / Q000821:virology; D008113:Liver Neoplasms / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics
chemical_list: D004273:DNA, Neoplasm; D012367:RNA, Viral