title: Prevention of cardiac fibrosis and left ventricular dysfunction in diabetic cardiomyopathy in rats by transgenic expression of the human tissue kallikrein gene.
abstract: Diabetic cardiomyopathy includes fibrosis. Kallikrein (KLK) can inhibit collagen synthesis and promote collagen breakdown. We investigated cardiac fibrosis and left ventricular (LV) function in transgenic rats (TGR) expressing the human kallikrein 1 (hKLK1) gene in streptozotocin (STZ) -induced diabetic conditions. Six weeks after STZ injection, LV function was determined in male Sprague-Dawley (SD) rats and TGR(hKLK1) (n=10/group) by a Millar tip catheter. Total collagen content (Sirius Red staining) and expression of types I, III, and VI collagen were quantified by digital image analysis. SD-STZ hearts demonstrated significantly higher total collagen amounts than normoglycemic controls, reflected by the concomitant increment of collagen types I, III, and VI. This correlated with a significant reduction of LV function vs. normoglycemic controls. In contrast, surface-specific content of the extracellular matrix, including collagen types I, III, and VI expression, was significantly lower in TGR(hKLK1)-STZ, not exceeding the content of SD and TGR(hKLK1) controls. This was paralleled by a preserved LV function in TGR(hKLK1)-STZ animals. The kallikrein inhibitor aprotinin and the bradykinin (BK) B2 receptor antagonist icatibant reduced the beneficial effects on LV function and collagen content in TGR(hKLK1)-STZ animals. Transgenic expression of hKLK1 counteracts the progression of LV contractile dysfunction and extracellular matrix remodeling in STZ-induced diabetic cardiomyopathy via a BK B2 receptor-dependent pathway.
authors.affiliation: Department of Cardiology and Pneumonology, Campus Benjamin Franklin, Charité-University Medicine, Free University of Berlin, Germany. ctschoepe@yahoo.com
authors.forename: Carsten
authors.initials: C
authors.lastname: Tschöpe
authors.forename: Thomas
authors.initials: T
authors.lastname: Walther
authors.forename: Jens
authors.initials: J
authors.lastname: Königer
authors.forename: Frank
authors.initials: F
authors.lastname: Spillmann
authors.forename: Dirk
authors.initials: D
authors.lastname: Westermann
authors.forename: Felicitas
authors.initials: F
authors.lastname: Escher
authors.forename: Matthias
authors.initials: M
authors.lastname: Pauschinger
authors.forename: Joao B
authors.initials: JB
authors.lastname: Pesquero
authors.forename: Michael
authors.initials: M
authors.lastname: Bader
authors.forename: Heinz-Peter
authors.initials: HP
authors.lastname: Schultheiss
authors.forename: Michel
authors.initials: M
authors.lastname: Noutsias
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D007611:Aprotinin / Q000494:pharmacology; D001920:Bradykinin / Q000031:analogs & derivatives* / Q000494:pharmacology; D065094:Bradykinin B2 Receptor Antagonists; D009202:Cardiomyopathies / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D003094:Collagen / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D006801:Humans; D007610:Kallikreins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D008297:Male; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000502:physiology; D013311:Streptozocin; D018487:Ventricular Dysfunction, Left / Q000209:etiology / Q000517:prevention & control*
chemical_list: D065094:Bradykinin B2 Receptor Antagonists; D011993:Recombinant Fusion Proteins; D013311:Streptozocin; C065679:icatibant; D003094:Collagen; D007611:Aprotinin; D007610:Kallikreins; D001920:Bradykinin