title: Relative contribution of target gene mutation and efflux to fluoroquinolone and erythromycin resistance, in French poultry and pig isolates of Campylobacter coli.
abstract: Thirty-eight avian and swine French isolates of Campylobacter coli were studied for their mechanisms of co-resistance to fluoroquinolones and erythromycin. A Thr86Ile modification of GyrA, responsible for fluoroquinolone resistance, was found in all the strains. Two different levels of resistance to erythromycin (MIC of 8-16 or >/=256 mg/l) were observed. A A2075G mutation in the 23S rRNA genes was found only in the highly-resistant strains. Phe-Arg-beta-naphthylamide, an efflux pump inhibitor, potentiated erythromycin in all the strains examined but restored susceptibility only in the strains with a low-level of resistance. This suggests the involvement of efflux in intrinsic and in acquired low-level of resistance to erythromycin in C. coli.
authors.affiliation: Institut National de la Recherche Agronomique, UR086 BioAgresseurs, Santé, Environnement, 37380 Nouzilly, France. payot@tours.inra.fr
authors.forename: Sophie
authors.initials: S
authors.lastname: Payot
authors.forename: Laetitia
authors.initials: L
authors.lastname: Avrain
authors.forename: Catherine
authors.initials: C
authors.lastname: Magras
authors.forename: Karine
authors.initials: K
authors.lastname: Praud
authors.forename: Axel
authors.initials: A
authors.lastname: Cloeckaert
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Chaslus-Dancla
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001692:Biological Transport / Q000187:drug effects; D017000:Campylobacter coli / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D016172:DNA Fingerprinting; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D004151:Dipeptides / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004357:Drug Synergism; D004917:Erythromycin / Q000378:metabolism / Q000494:pharmacology*; D024841:Fluoroquinolones / Q000378:metabolism / Q000494:pharmacology*; D005516:Food Microbiology; D005602:France; D005798:Genes, Bacterial; D020459:Genes, rRNA; D049990:Membrane Transport Modulators; D026901:Membrane Transport Proteins / Q000037:antagonists & inhibitors; D008826:Microbial Sensitivity Tests; D009154:Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011200:Poultry / Q000382:microbiology*; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D013552:Swine / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004151:Dipeptides; D024841:Fluoroquinolones; D049990:Membrane Transport Modulators; D026901:Membrane Transport Proteins; D012338:RNA, Ribosomal, 23S; C474925:phenylalanylarginine-naphthylamide; D004917:Erythromycin; D027081:DNA Gyrase