title: Cloning, functional characterization, and near-ultraviolet radiation-enhanced expression of a photolyase gene (PHR1) from the phytopathogenic fungus Bipolaris oryzae.
abstract: Photolyase is a DNA repair enzyme that can absorb blue/ultraviolet A light as energy and split a pyrimidine dimer induced by ultraviolet radiation. We isolated and characterized PHR1, a gene encoding photolyase, from the phytopathogenic fungus Bipolaris oryzae. Sequence analysis showed that PHR1 encodes a putative protein that has 634 amino acids, a molecular mass of 72.6 kDa, and 51.3-55.5% sequence identity to other fungal photolyases. Complementation of the photoreactivation-deficient Escherichia coli mutant by PHR1 cDNA demonstrated that the PHR1 gene from B. oryzae encodes a functional photolyase. Real-time PCR analysis showed that the PHR1 transcripts were specifically enhanced by near-ultraviolet radiation (300-400 nm) and by sunlight.
authors.affiliation: Faculty of Life and Environmental Science, Shimane University, Matsue, Shimane, 690-8504, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Kihara
authors.forename: Akihiro
authors.initials: A
authors.lastname: Moriwaki
authors.forename: Nobuhito
authors.initials: N
authors.lastname: Matsuo
authors.forename: Sakae
authors.initials: S
authors.lastname: Arase
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Honda
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014466:Ultraviolet Rays; D015854:Up-Regulation
chemical_list: D005656:Fungal Proteins; C437712:UVI-1 protein, Bipolaris oryzae; D004255:Deoxyribodipyrimidine Photo-Lyase