title: Bone formation using novel interconnected porous calcium hydroxyapatite ceramic hybridized with cultured marrow stromal stem cells derived from Green rat.
abstract: The clinical use of cultured marrow stromal stem cells (MSCs) has recently attracted attention in the field of tissue engineering. For the clinical use of the MSCs, a prominent scaffold is needed. A scaffold hybridized with MSCs is transformed into a "bioactive bone substitute," and this provides good osteoconduction. In this study, a novel calcium hydroxyapatite ceramic with an interconnected porous structure (IP-CHA) was used as a scaffold. MSCs were harvested from Green rats containing Green Fluorescent Protein (GFP), and then these hybrids were implanted into the tibias of Sprague-Dawley rats. The purposes of this study were to examine the osteogenic ability of these hybrids without coculture, and to evaluate whether the resulting bone formation originated from the grafted MSCs or the recipient's cells. The hybridized group showed excellent bone formation compared with the IP-CHA-only implant group. Observation of the implanted MSCs revealed that they survived 8 weeks after surgery, and differentiated into osteoblast-like cells, thus providing bone formation. This implantation of the MSCs/IP-CHA composite provides excellent osteoconduction, and is expected to have extensive clinical applications.
authors.affiliation: Department of Orthopedic Surgery, Division of Clinical Medical Science, Programs for Applied Biomedicine, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan. yoito@hiroshima-u.ac.jp
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Ito
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Tanaka
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Fujimoto
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yasunaga
authors.forename: Osamu
authors.initials: O
authors.lastname: Ishida
authors.forename: Muhammad
authors.initials: M
authors.lastname: Agung
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Ochi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D015415:Biomarkers; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D001857:Bone Matrix / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry / Q000378:metabolism*; D017886:Durapatite / Q000737:chemistry / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis / Q000502:physiology*; D019736:Prostheses and Implants; D051381:Rats; D011919:Rats, Inbred Strains; D023822:Tissue Engineering / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D015415:Biomarkers; D049452:Green Fluorescent Proteins; D017886:Durapatite