title: [Microbiological findings in patients with recurrent vulvovaginal candidiasis in the Hradec Králové Faculty Hospital 1995-2002].
abstract: OBJECTIVE
  To evaluate the microbiological findings in the patients with the recurrent vulvovaginal candidiasis (RVVC) with a focus on the establishment of fungal etiology and its in vitro antifungal susceptibility.
  
  
  METHODS
  Retrospective clinical and laboratory study.
  
  
  METHODS
  Department of Obstetrics and Gynecology, Medical Faculty Hradec Králové, Charles University, Prague, Department of Clinical Microbiology, Medical Faculty Hradec Králové, Charles University, Prague, Department of Biological and Medical Sciences, Faculty of Pharmacy Hradec Králové, Charles University, Prague.
  
  
  METHODS
  An analysis of clinical and anamnestic data in outpatients of the Dept. of Obstetrics and Gynecology and the laboratory data from the microbiological examinations performed in the Dept. of Clinical Microbiology from 1995 to 2002.
  
  
  RESULTS
  Candida albicans accounted for 88.5% of the episodes of RVVC in the setting of 56 patients. Non-albicans Candida species were represented especially by C. glabrata (4.9%) and C. krusei (3.1%). There were no considerable differences between the spectrum of RVVC and acute vulvovaginal candidiasis with the exception of Saccharomyces cerevisiae (0.7% in RVVC vs. 3.7% in acute VVC). Mycological findings in 61 (20.5%) samples were accompanied by bacterial microbiota with the predominance of Streptococcus agalactiae (n = 15) and Gardnerella vaginalis (n = 9). Decreased antifungal susceptibility determined by the disk test was observed in the strains of C. glabrata, C. krusei and S. cerevisiae, the other yeast isolates being susceptible to all ten antifungal drugs tested.
  
  
  CONCLUSIONS
  The microbiological examination was decisive for the establishment of the diagnosis of RVVC in most cases. The most frequent etiological agents responsible for the attacks of RVVC as well as for acute vulvovaginal candidiasis was C. albicans, which was generally susceptible to antifungal drugs.
authors.affiliation: Ustav klinické mikrobiologie, LF UK a FN, Hradec Králové.
authors.forename: V
authors.initials: V
authors.lastname: Buchta
authors.forename: J
authors.initials: J
authors.lastname: Spacek
mesh_terms: D000208:Acute Disease; D000935:Antifungal Agents / Q000494:pharmacology; D001419:Bacteria / Q000302:isolation & purification; D002175:Candida / Q000302:isolation & purification; D002181:Candidiasis, Vulvovaginal / Q000382:microbiology*; D005260:Female; D006801:Humans; D008826:Microbial Sensitivity Tests; D012008:Recurrence; D012441:Saccharomyces cerevisiae / Q000302:isolation & purification; D014621:Vagina / Q000382:microbiology*
chemical_list: D000935:Antifungal Agents