Determination of minimal regrowth concentration (MRC) in clinical isolates of various biofilm-forming bacteria.
Based on the ability to attach to polymeric surfaces, the formation of biofilms was determined in 5 wild-type strains (Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumanii, Escherichia coli, Staphylococcus warneri). Using modified Christensen method, minimum regrowth concentration (MRC) of piperacillin, piperacillin-tazobactam, cefoperazon, ceftazidim, cefepim, meronem, ciprofloxacin, netilmicin and amikacin for Gram-negative and of ampicillin-sulbactam, chloramphenicol, tetracycline, clindamycin, vancomycin and teicoplanin for Gram-positive bacteria was estimated in trypticase-soy broth medium after a 1-d growth on polystyrene microtiter plates. Adherent bacterial populations exhibited reduced antimicrobial susceptibility, which was not shown in submerged cultures. Our results indicate that MRC can predict therapeutic outcome of antibiotic treatment better than the minimum inhibitory concentration tests commonly used.
Cernohorská
L
L
Department of Microbiology, Masaryk University Medical School, St. Anna's Faculty Hospital, 656 91 Brno, Czechia. lenka.cernohorska@fnusa.cz
Votava
M
M
D040981:Acinetobacter baumannii / Q000187:drug effects / Q000254:growth & development; D000583:Amikacin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development*; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D002701:Chloramphenicol / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology; D002981:Clindamycin / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000254:growth & development; D007769:Lactams / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009428:Netilmicin / Q000494:pharmacology; D011237:Predictive Value of Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D013210:Staphylococcus / Q000187:drug effects / Q000254:growth & development; D017334:Teicoplanin / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology; D014640:Vancomycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D007769:Lactams; D002981:Clindamycin; D009428:Netilmicin; D002939:Ciprofloxacin; D017334:Teicoplanin; D002701:Chloramphenicol; D014640:Vancomycin; D000583:Amikacin; D013752:Tetracycline