Identification of immunoreactive proteins of Trichinella spiralis adult and adult excretory/secretory (E/S) antigens in sera of human and animals.
Trichinella spiralis adult and adult excretory/secretory antigens were evaluated by ELISA and Western blot techniques for the serological diagnosis of trichinellosis in naturally infected human, swine and experimentally infected rats. Blood samples were collected from 16 symptomatic patients at hospital and 12 asymptomatic individuals working at the swine slaughter-house. Blood samples were also obtained from 75 pigs at two abattoirs and experimentally infected rats (day 25 PI). There was no great difference in the seroprevalence of trichinellosis between symptomatic (56%) and asymptomatic (50%) individuals. ELISA results recorded that 13% of swine was seropositive, while 44% were suspected to be infected with T. spiralis. Immunoblotting profiles of T. spiralis adult antigen against human, swine and rat sera showed common reactive bands at 95.00 and 64.466 KDa (human and swine), and 35.554 KDa (human and rat), while the blotting patterns of adult E/S products against human, swine and rat antibodies recognized two trichinellosis-specific determinants between human and swine (87.619 & 74.136 KDa) and between human and rat (98.00 & 16.535 KDa). It can be suggested that a 45.00, 75.355 & 25.389, and a 57.989 KDa polypeptides of adult antigen were diagnostic for human, swine and rat trichinellosis, respectively. While a 26.00, 24.00, and 46.994 KDa proteins of adult E/S products were diagnostic for human, swine and rat trichinellosis, respectively.
Hassanain
Mohey A
MA
Department of Parasitology and Animal Diseases, National Research Center, Dokki, Giza, Egypt.
Hassanain
Nawal A
NA
El-Mogazy
Faragalla M
FM
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000097:blood* / Q000276:immunology; D015153:Blotting, Western / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D008297:Male; D008970:Molecular Weight; D051381:Rats; D016036:Seroepidemiologic Studies; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth