Construction and isolation of recombinant MVA.
Modified vaccinia virus Ankara (MVA) is a valuable tool for the expression of recombinant genes used for such purposes as the study of protein functions or characterization of cellular and humoral immune responses. A major advantage of MVA is its clear safety record, and it can be handled under biosafety level 1 conditions. Despite its replication deficiency in human and most mammalian cells, MVA provides high-level gene expression and has proven to be immunogenic when delivering heterologous antigens in animals and humans. This chapter provides state-of-the-art protocols for generation, plaque isolation, molecular characterization, as well as amplification and purification of MVA vector viruses to obtain recombinant viruses for further evaluation.
Staib
Caroline
C
Institute of Molecular Virology, GSF-Research Center and Technical University Munich, Munich, Germany.
Drexler
Ingo
I
Sutter
Gerd
G
D000818:Animals; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D011995:Recombination, Genetic; D014616:Vaccinia virus / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics*
D014764:Viral Proteins