Reduction of 1-methyl-1-nitrosourea-induced tumor burden with DNA vaccines encoding mutated ras epitopes and the costimulatory molecule B7.1.
1-methyl-1-nitrosourea (MNU), a well characterized carcinogen, was used to induce adenocarcinomas in rat mammary gland. 150 days after the first injection of MNU, the animals were treated with DNA minigene vaccines encoding ras T cell epitopes together with the co-stimulatory molecule B7.1 (CD 80). Five injections with a biolistic device (gene gun) in monthly intervals significantly reduced the tumor burden. A therapeutic effect could be measured with both, DNA vaccines encoding ras epitopes and B7.1, as well as with a DNA vaccine expressing solely the B7.1 molecule thus indicating the potential of genetic vaccination for turnor treatment.
Mostböck
S
S
Institute of Experimental Endocrinology, Slovak Academy of Science, Bratislava, Slovakia.
Macejová
D
D
Baranová
M
M
Weiss
R
R
Scheiblhofer
S
S
Thalhamer
J
J
Brtko
J
J
D000230:Adenocarcinoma / Q000139:chemically induced / Q000235:genetics / Q000276:immunology / Q000628:therapy*; D000818:Animals; D018122:B7-1 Antigen / Q000235:genetics* / Q000276:immunology / Q000627:therapeutic use*; D001943:Breast Neoplasms / Q000139:chemically induced / Q000235:genetics / Q000276:immunology / Q000628:therapy*; D019496:Cancer Vaccines / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use; D002273:Carcinogens; D005240:Feasibility Studies; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D007114:Immunization / Q000379:methods; D008770:Methylnitrosourea; D016297:Mutagenesis, Site-Directed; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome; D019444:Vaccines, DNA / Q000235:genetics / Q000627:therapeutic use*
D018122:B7-1 Antigen; D019496:Cancer Vaccines; D002273:Carcinogens; D019444:Vaccines, DNA; D008770:Methylnitrosourea