Osmotic stress leads to decreased intracellular pH of Listeria monocytogenes as determined by fluorescence ratio-imaging microscopy.
Intracellular pH (pH(i)) of Listeria monocytogenes was determined after exposure to NaCl or sorbitol in liquid and solid media (agar). Both compounds decreased pH(i), and recovery on solid medium was impaired compared to that in liquid medium. N,N'-dicyclohexylcarbodiimide abolished pH(i) recovery, and lowering a(w) with glycerol showed no effect on pH(i).
Fang
Weihuan
W
College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310029, People's Republic of China.
Siegumfeldt
Henrik
H
Budde
Birgitte Bjørn
BB
Jakobsen
Mogens
M
D003470:Culture Media; D018869:Heat-Shock Response; D006863:Hydrogen-Ion Concentration; D008089:Listeria monocytogenes / Q000502:physiology*; D008856:Microscopy, Fluorescence / Q000379:methods; D009997:Osmotic Pressure; D012965:Sodium Chloride / Q000494:pharmacology*; D013012:Sorbitol / Q000494:pharmacology*
D003470:Culture Media; D012965:Sodium Chloride; D013012:Sorbitol