Localization by indirect immunofluorescence of tetrin, actin, and centrin to the oral apparatus and buccal cavity of the macrostomal form of Tetrahymena vorax.
We have taken advantage of the size of the macrostomal oral apparatus of Tetrahymena vorax to investigate the immunofluorescent localization of three cytoskeletal proteins--tetrin, actin, and centrin. Tetrin and actin antibodies co-localize to cross-connectives that anchor the membranelles. These antibodies also recognize the coarse filamentous reticulum, a filament associated with the undulating membrane. Actin-specific localization extends beyond the coarse filamentous reticulum-undulating membrane complex into a region called the specialized cytoplasm. A centrin antibody localizes to the fine filamentous reticulum which, along with microtubules of the oral ribs, circumscribes the cytostomal opening. Models of phagocytic contraction based on these data are presented.
McLaughlin
Neil B
NB
Department of Biological Sciences, University of Illinois at Chicago, 845 West Taylor Street, Chicago, Illinois 60607-7060, USA. nmclau1@uic.edu
Buhse
Howard E
HE
D000199:Actins / Q000032:analysis* / Q000276:immunology; D000818:Animals; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000276:immunology; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis* / Q000276:immunology; D003598:Cytoskeletal Proteins / Q000032:analysis* / Q000276:immunology; D003599:Cytoskeleton / Q000737:chemistry; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000737:chemistry; D010587:Phagocytosis; D015800:Protozoan Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D013768:Tetrahymena / Q000737:chemistry* / Q000166:cytology
D000199:Actins; D002135:Calcium-Binding Proteins; D002868:Chromosomal Proteins, Non-Histone; D003598:Cytoskeletal Proteins; D015800:Protozoan Proteins; C418785:tetrin A protein, Tetrahymena; C418786:tetrin B protein, Tetrahymena; C418787:tetrin C protein, Tetrahymena thermophila; C056608:caltractin