title: Protective immunity of SpaA-antigen producing Lactococcus lactis against Erysipelothrix rhusiopathiae infection.
abstract: OBJECTIVE
  To develop an economical, safe and simple vaccination system against swine erysipelas using SpaA-antigen producing Lactococcus lactis.
  
  
  RESULTS
  The spaA gene of Erysipelothrix rhusiopathiae was inserted into a shuttle plasmid pSECE1 to construct pSECE1.3. The SpaA produced in L. lactis maintained a stable antigenicity without degrading in growth. After mice were inoculated intranasally and orally with pSECE1.3-carrying L. lactis cells, IgG and IgA specific to SpaA were detected, and all the mice survived a challenge with 100 LD(50) of E. rhusiopathiae Tama-96 in the inner thigh.
  
  
  CONCLUSIONS
  SpaA-producing L. lactis appears useful as an effective subunit vaccine against swine erysipelas.
  
  
  CONCLUSIONS
  In this vaccination system, purification of the antigen and injection are unnecessary, leading to a reduced production cost, reduced labour and less stress to the animals. This vaccination system of the lactic acid bacteria should be a safe and suitable vehicle for a polyvalent vaccine.
authors.affiliation: Research Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
authors.forename: H I
authors.initials: HI
authors.lastname: Cheun
authors.forename: K
authors.initials: K
authors.lastname: Kawamoto
authors.forename: M
authors.initials: M
authors.lastname: Hiramatsu
authors.forename: H
authors.initials: H
authors.lastname: Tamaoki
authors.forename: T
authors.initials: T
authors.lastname: Shirahata
authors.forename: S
authors.initials: S
authors.lastname: Igimi
authors.forename: S-I
authors.initials: SI
authors.lastname: Makino
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D005260:Female; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D013294:Lactococcus lactis / Q000276:immunology*; D051379:Mice; D010957:Plasmids; D013552:Swine; D013554:Swine Erysipelas / Q000276:immunology / Q000517:prevention & control*; D014611:Vaccination / Q000379:methods; D019444:Vaccines, DNA / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; C114457:SpaA protein, Erysipelothrix rhusiopathiae; D019444:Vaccines, DNA