title: Application of preparative high-speed counter-current chromatography for the separation of flavonoids from the leaves of Byrsonima crassa Niedenzu (IK).
abstract: The methanolic extract of the leaves of the medicinal plant Byrsonima crassa (Malpighiaceae) contain flavonoids with antioxidant activity. They were separated in a preparative scale using high-speed counter-current chromatography. The optimum solvent system used was composed of a mixture of ethyl acetate-n-propanol-water (140:8:80 (v/v/v)) and led to a successful separation between monoglucosilated flavonoids (quercetin-3-O-alpha-L-arabinoside, quercetin-3-O-beta-D-galactoside) and the biflavonoid amentoflavone in only 3.5 h. The purities of quercetin-3-O-alpha-L-arabinoside (95 mg), quercetin-3-O-beta-D-galactoside (16 mg) and the biflavonoid amentoflavone (114 mg) were all isolated at purity over 95%. Identification was performed by 1H NMR, 13C NMR and UV analyses.
authors.affiliation: Departamento de Química Orgânica, Instituto de Química, UNESP, Rua Francisco Degni s/n, Bairro Quitandinha, CP 355, CEP 14800-900 Araraquara, SP, Brazil. miriamsannomiya@pop.com.br
authors.forename: Miriam
authors.initials: M
authors.lastname: Sannomiya
authors.forename: Clenilson M
authors.initials: CM
authors.lastname: Rodrigues
authors.forename: Roberta G
authors.initials: RG
authors.lastname: Coelho
authors.forename: Lourdes C
authors.initials: LC
authors.lastname: dos Santos
authors.forename: Clélia A
authors.initials: CA
authors.lastname: Hiruma-Lima
authors.forename: Alba R M
authors.initials: AR
authors.lastname: Souza Brito
authors.forename: Wagner
authors.initials: W
authors.lastname: Vilegas
mesh_terms: D003377:Countercurrent Distribution / Q000379:methods*; D005419:Flavonoids / Q000302:isolation & purification*; D029581:Malpighiaceae / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005419:Flavonoids