Fatty acid pattern, oxidation product development, and antioxidant loss in muscle tissue of rainbow trout and Dicentrarchus labrax during growth.
The levels of hydrophilic, lipophilic, and enzymatic antioxidants, the oxidative damage to lipids and proteins, and the fatty acid patterns of triglyceride and phospholipid fractions were assayed in fresh muscle tissue of rainbow trouts (Oncorhynchus mykiss) and sea basses (Dicentrarchus labrax) during aging, to investigate the correlation between oxidative stress and aging processes in fish. The present studies suggests that lipid peroxidation and accumulation of oxidized proteins during in vivo aging are most likely to be linked with an age-dependent decline of lipophilic antioxidants (CoQH(2), CoQ, and vitamin E) and vitamin C contents in muscle tissue, whereas fish aging is not linked to a decline in antioxidant enzymes and reduced glutathione levels. Lipophilic antioxidant and vitamin C levels represent a reliable marker of oxidative stress during aging, and their determination might be useful for the assessment of fish age.
Passi
Siro
S
Istituto Dermopatico dell'Immacolata (IDI, IRCCS), Via Monti di Creta, 104, Roma, Italy.
Ricci
Riccardo
R
Cataudella
Stefano
S
Ferrante
Irene
I
De Simone
Francesco
F
Rastrelli
Luca
L
D000375:Aging; D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D001205:Ascorbic Acid / Q000032:analysis; D001492:Bass / Q000254:growth & development*; D005227:Fatty Acids / Q000032:analysis*; D015227:Lipid Peroxidation; D008055:Lipids / Q000032:analysis; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000254:growth & development*; D017686:Oncorhynchus mykiss / Q000254:growth & development*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011506:Proteins / Q000032:analysis / Q000737:chemistry
D000975:Antioxidants; D005227:Fatty Acids; D008055:Lipids; D011506:Proteins; D001205:Ascorbic Acid