title: Pulsed field gel electrophoresis of related Escherichia coli O157 isolates associated with beef cattle and comparison with unrelated isolates from animals, meats and humans.
abstract: The pulsed field gel electrophoresis (PFGE) diversity of 51 related Escherichia coli O157 isolates, associated with beef cattle from a single-farm-to-single abattoir (SF-SA) chain of events was determined. The 51 related E. coli O157 isolates from hides, faeces or carcasses of SF-SA cattle produced 11 different PFGE profiles. Also, the PFGE diversity of 6 isolates, associated with a second cattle abattoir, was determined; only two PFGE profiles were found. On the other hand, the PFGE diversity of 136 unrelated E. coli O157 isolates (from healthy meat animals, retail meats and cases of human disease) was also determined. The 136 unrelated E. coli O157 isolates produced 78 different PFGE profiles, most of which (approximately 70%) comprised only one isolate. Overall, the results showed: (a) related E. coli O157 isolates (from both SF-SA events, and the second abattoir) had a markedly narrower clonal profile than the 136 unrelated E. coli O157 isolates; (b) the isolation of identical PFGE types from hide, lairage environment, and carcasses confirms the significance of cross-contamination (both pre-slaughter and during skinning) taking place at abattoirs; and (c) PFGE typing of isolates offers a good tool for tracking sources/routes of such cross-contamination. Such cross-contamination may lead to originally E. coli O157-free animals (and resultant carcasses) becoming contaminated during farm-slaughter-dressing chain of events, so development of efficient control strategies is required.
authors.affiliation: Microbiology Department, Direct Laboratory Services Ltd., Wergs Road, Wolverhampton WV6 8TQ, UK. sheryl.avery@directlabs.co.uk
authors.forename: S M
authors.initials: SM
authors.lastname: Avery
authors.forename: E
authors.initials: E
authors.lastname: Liebana
authors.forename: M L
authors.initials: ML
authors.lastname: Hutchison
authors.forename: S
authors.initials: S
authors.lastname: Buncic
mesh_terms: D000003:Abattoirs; D000818:Animals; D002417:Cattle / Q000382:microbiology*; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D019453:Escherichia coli O157 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D005506:Food Contamination / Q000032:analysis* / Q000517:prevention & control; D005511:Food Handling / Q000379:methods; D005516:Food Microbiology; D006801:Humans; D008460:Meat / Q000382:microbiology*; D022603:Shiga Toxins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D022603:Shiga Toxins