title: Study in mono and bilayers of the interaction of hepatitis G virus (GBV-C/HGV) synthetic antigen E2(99-118) with cell membrane phospholipids.
abstract: The interaction of the hepatitis G synthetic peptide E2(99-118) with cell membrane phospholipids of different characteristics such as dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylglycerol (DPPG) was studied by Langmuir isotherms. Epifluorescence microscopy and Atomic force microscopy (AFM) was also used to study interactions with DPPC. Compression isotherms of DPPC/E2(99-118) and DPPG/E2(99-118) mixed monolayers showed negative deviation from ideallity consistent with the existence of attractive interactions. The incorporation of the peptide in DPPC monolayer was also confirmed in epifluorescence microscopy and AFM studies. The peptide retarded the formation of DPPC domains and did not let the phospholipid get organized. No important differences in the interactions with DPPC (neutral) or DPPG (anionic) were found, thus suggesting that electrostatics forces do not have a predominant influence in these interactions.
authors.affiliation: Department of Physiochemistry, Faculty of Pharmacy, University of Barcelona, Av. Joan XXIII s.n., 08028 Barcelona, Spain.
authors.forename: Macarena
authors.initials: M
authors.lastname: Dastis
authors.forename: Nuria
authors.initials: N
authors.lastname: Rojo
authors.forename: M A
authors.initials: MA
authors.lastname: Alsina
authors.forename: Isabel
authors.initials: I
authors.lastname: Haro
authors.forename: Amiya Kumar
authors.initials: AK
authors.lastname: Panda
authors.forename: Concepció
authors.initials: C
authors.lastname: Mestres
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D000956:Antigens, Viral / Q000737:chemistry*; D002462:Cell Membrane / Q000378:metabolism*; D004347:Drug Interactions; D029407:GB virus C / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D010455:Peptides / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D000956:Antigens, Viral; D008051:Lipid Bilayers; D010455:Peptides; D010715:Phosphatidylglycerols; D010743:Phospholipids; D015060:1,2-Dipalmitoylphosphatidylcholine; C030345:1,2-dipalmitoylphosphatidylglycerol