Depth-resolved structural imaging by third-harmonic generation microscopy.
Third harmonic generation microscopy is shown to be a robust method for obtaining structural information on a variety of biological specimens. Its nature allows depth-resolved imaging of inhomogeneities with virtually no background from surrounding homogeneous media. With an appropriate illumination geometry, third harmonic generation microscopy is shown to be particularly suitable for imaging of biogenic crystals, enabling extraction of the crystal orientation.
Oron
Dan
D
Department of Physics of Complex Systems, Weizmann Institute of Science, Rehovot IL-76100, Israel. dan.oron@weizmann.ac.il
Yelin
Dvir
D
Tal
Eran
E
Raz
Sefi
S
Fachima
Rachel
R
Silberberg
Yaron
Y
D000818:Animals; D004625:Embryo, Nonmammalian / Q000033:anatomy & histology; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D009865:Oocytes / Q000166:cytology; D001744:Urinary Bladder Calculi / Q000648:ultrastructure