Novel fluorescent biosensor for pathogenic toxins using cyclic polypeptide conjugates.
This work describes a two-step conjugate synthesis of a new fluorescent analog of microcystin-LR and its subsequent utilization for the development of an optical biosensor for cyanobacteria toxins. The biosensor concept is based on the competitive binding between the native microcystin and its fluorescent analog at immobilized alkaline phosphatase enzymes.
Sadik
Omowunmi A
OA
Department of Chemistry, State University of New York at Binghamton, P. O. Box 6000, Binghamton, NY 13902-6000, USA.
Yan
Fei
F
D001427:Bacterial Toxins / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D001667:Binding, Competitive; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D000458:Cyanobacteria / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D008387:Marine Toxins; D052998:Microcystins; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism
D001427:Bacterial Toxins; D005456:Fluorescent Dyes; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; D010749:Phosphoprotein Phosphatases; C057862:cyanoginosin LR