title: Studying vaccinia virus RNA processing in vitro.
abstract: The study and use of vaccinia virus-derived RNA-modifying proteins has made a significant contribution to molecular biology. Here, the purification and assay of two such proteins, comprising the vaccinia poly(A) polymerase/cap-specific mRNA 2'-O-methyltransferase, is described.
authors.affiliation: Institute of Biosciences and Technology, Center for Genome Research, Texas A&M University Health Science Center, Houston, TX, USA.
authors.forename: Paul D
authors.initials: PD
authors.lastname: Gershon
mesh_terms: D002460:Cell Line; D066298:In Vitro Techniques; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D014616:Vaccinia virus / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D009713:Nucleotidyltransferases