Isolation and characterization of a generalized transducing phage for Pseudomonas aeruginosa strains PAO1 and PA14.
A temperate, type IV pilus-dependent, double-stranded DNA bacteriophage named DMS3 was isolated from a clinical strain of Pseudomonas aeruginosa. A clear-plaque variant of this bacteriophage was isolated. DMS3 is capable of mediating generalized transduction within and between P. aeruginosa strains PA14 and PAO1, thus providing a useful tool for the genetic analysis of P. aeruginosa.
Budzik
Jonathan M
JM
Dartmouth College, Hanover, New Hampshire 03755, USA.
Rosche
William A
WA
Rietsch
Arne
A
O'Toole
George A
GA
D010861:Fimbriae, Bacterial / Q000502:physiology; D017105:Pseudomonas Phages / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000821:virology*; D011991:Receptors, Virus / Q000502:physiology; D014161:Transduction, Genetic
D011991:Receptors, Virus