Regulating effects of porcine interleukin-6 gene and CpG motifs on immune responses to porcine trivalent vaccines in mice.
In order to develop novel immunoadjuvants to boost immune response of conventional vaccines, experiments were conducted to investigate the regulating effects of porcine interleukin-6 gene and CpG motifs as the molecular adjuvants on immune responses of mice that were co-inoculated with trivalent vaccines against Swine fever, the Pasteurellosis and Erysipelas suis. Synthetic oligodeoxynuleotides containing CpG motifs were ligated into pUC18, forming recombinant pUC18-CpG plasmid. Eukaryotic plasmid expressing porcine interleukin-6 (VPIL-6) were also constructed as molecular adjuvants in an attempt to enhance levels of immune responses of mice co-administered with the trivalent vaccines in this paper. The cellular and humoral immune responses of mice were systematically analysed, and the experimental results were observed that the number of white blood cells, monocytes, granuloytes and lymphocytes significantly increased, respectively, in the mice immunized with VPIL-6, compared with those of the control; the IgG content and titre of specific antibodies to the trivalent vaccine mounted remarkably in the sera from the VPIL-6 vaccinated mice; the proliferation of lymphocytes and induced IL-2 activities were significantly increased in the vaccinated groups. The above-mentioned immune responses of mice co-inoculated with pUC18-CpG plasmid were significantly stronger than those of co-inoculated with pUC18 plasmid, suggesting that the immunostimulatory effect of oligodeoxynuleotides CpG is closely connected with the number of CpG motifs. These results suggest that the porcine IL-6 gene and CpG motifs could be employed as effective immunoadjuvants to elevate immunity to conventional vaccines.
Wu
Mei
M
National Lab of Biocontrol Engineering of Grassland Pests, Life Science College, Sichuan University, Chendu, Sichuan 610064, PR China. wumei8012@yahoo.com.cn
Gao
Rong
R
Meng
Mingjie
M
Li
Jiangling
J
Tang
Manshu
M
Tan
Manshu
M
Shen
Yi
Y
Wang
Lihuan
L
Yin
Xue
X
Wu
Xueyan
X
Xie
Hongguan
H
Liu
Shigui
S
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000917:Antibody Formation / Q000502:physiology; D006692:Classical Swine Fever Virus / Q000276:immunology; D004886:Erysipelas / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D007111:Immunity, Cellular / Q000502:physiology; D015850:Interleukin-6 / Q000235:genetics* / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D010325:Pasteurella / Q000276:immunology; D010957:Plasmids; D013552:Swine; D014611:Vaccination; D017778:Vaccines, Combined / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; C408982:CPG-oligonucleotide; D015850:Interleukin-6; D009838:Oligodeoxyribonucleotides; D017778:Vaccines, Combined; D014765:Viral Vaccines