title: Radionuclide imaging of mesenchymal stem cells transplanted into spinal cord.
abstract: In vivo tracking of stem cells implanted to spinal cord by radionuclide imaging was investigated. The high expression of transferrin receptor on human mesenchymal stem cells (hMSCs) was verified by flow cytometry, radioligand binding and immunofluorescence. Radiolabelled transferrin was chosen as a tracer for scintigraphic imaging of the hMSCs transplanted into spinal cord of rabbits. Comparative experiments with radiolabelled human serum albumin as tracer and PBS as graft as well as ex vivo autoradiography demonstrated the specific uptake of radiolabelled transferrin of hMSCs. hMSCs could be detected in vivo with radiolabelled transferrin targeting at cellular transferrin receptors at an early stage after transplantation into spinal cord.
authors.affiliation: Department of Orthopedics, Peking University Third Hospital, Beijing 100083, China.
authors.forename: Jinzhu
authors.initials: J
authors.lastname: Bai
authors.forename: Weimin
authors.initials: W
authors.lastname: Ding
authors.forename: Minjun
authors.initials: M
authors.lastname: Yu
authors.forename: Jin
authors.initials: J
authors.lastname: Du
authors.forename: Zhongjun
authors.initials: Z
authors.lastname: Liu
authors.forename: Bing
authors.initials: B
authors.lastname: Jia
authors.forename: Lingsong
authors.initials: L
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.forename: Jiahe
authors.initials: J
authors.lastname: Tian
authors.forename: Fan
authors.initials: F
authors.lastname: Wang
mesh_terms: D000818:Animals; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000000981:diagnostic imaging*; D011817:Rabbits; D011877:Radionuclide Imaging; D013116:Spinal Cord / Q000000981:diagnostic imaging* / Q000637:transplantation*; D033581:Stem Cell Transplantation / Q000379:methods*