Postmortem effect of pentobarbital anesthetic on survival of adult cortical neurons in primary culture.
We determined whether pentobarbital anesthetic is required to culture postmortem adult rat neurons. Pentobarbital treatment resulted in two-fold increases in neuron survival in culture after 2 and 4 h postmortem compared to non-anesthetic controls, but was not as effective as simple postmortem treatment on ice and therefore not essential.
Viel
John J
JJ
Department of Medical Microbiology and Immunology, School of Medicine, Southern Illinois University, Springfield, IL 62794-9626, USA.
McManus
Dennis Q
DQ
Brewer
Gregory J
GJ
D000759:Adjuvants, Anesthesia / Q000494:pharmacology; D000818:Animals; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D002540:Cerebral Cortex / Q000166:cytology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007053:Ice; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D010424:Pentobarbital / Q000494:pharmacology*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D011180:Postmortem Changes; D051381:Rats; D013997:Time Factors
D000759:Adjuvants, Anesthesia; D005904:Glial Fibrillary Acidic Protein; D007053:Ice; D010751:Phosphopyruvate Hydratase; D010424:Pentobarbital