title: [Cloning of human amelogenin gene encoding mature peptide].
abstract: OBJECTIVE
  The purpose of this study was to clone human amelogenin gene encoding mature protein, which provides a basis for expressing the recombinant human amelogenin in Escherichia coli. in the future.
  
  
  METHODS
  In this study, total RNA was extracted from the dental germ of a legally aborted embryo by Trizol. Using RT-PCR technique we obtained synthesis of cDNA from the total RNA, and the desired DNA products were conducted with PCR from cDNA. The segment was inserted into expression vector PQE30 and the interesting plasmid was transformed into Escherichia coli. host DH5alpha. The double-stranded DNA of positive clone was analyzed by PCR, restriction endonuclease mapping and DNA sequence analysis.
  
  
  RESULTS
  The sequence analysis of recombinant plasmid showed that the human amelogenin encoding mature protein was inserted into vector PQE30 accurately.
  
  
  CONCLUSIONS
  We conducted human amelogenin encoding mature protein from dental germ of a legally aborted embryo and got the recombinant plasmid which may express amelogenin gene for further research.
authors.affiliation: Department of Operation Dentistry,School of Stomatology,Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Cheng
authors.forename: Jian-qiang
authors.initials: JQ
authors.lastname: Lei
authors.forename: Qi-quan
authors.initials: QQ
authors.lastname: Zhu
authors.forename: Hong-hai
authors.initials: HH
authors.lastname: Wang
authors.forename: Rong
authors.initials: R
authors.lastname: Shu
mesh_terms: D053523:Amelogenin; D003001:Cloning, Molecular; D003746:Dental Enamel Proteins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008970:Molecular Weight; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D053523:Amelogenin; D003746:Dental Enamel Proteins; D011994:Recombinant Proteins