[Effect of microwave irradiation on neurocyte mitochondrial ultrastructure and mtTFA mRNA expression in rats cerebral cortex and hippocampus].
OBJECTIVE
To explore molecular controlling mechanism of mitochondrial injury induced by different density of microwave irradiation.


METHODS
Rats were exposed to microwave irradiation for 1 hour at average power density of 3 mW/cm(2) or 30 mW/cm(2). After microwave irradiation, the changes of pathological ultrastructure of rat cerebral cortex and hippocampus were observed by electron microscope, and mitochondrial transcription factor A (mtTFA) mRNA expression level were determined by RT-PCR.


RESULTS
After 3 mW/cm(2) microwave irradiation for 0, 3, 24 h, mitochondrial ultrastructure and mtTFA mRNA expression level didn't significantly change in rat cerebral cortex and hippocampus. After 30 mW/cm(2) microwave irradiation for 0, 3, 24 h, mitochondrial ultrastructure obviously changed, mtTFA mRNA expression in rat hippocampus significantly increased by 67.00%, 80.00%, 30.00% respectively, and in rat cerebral cortex by 133.00%, 86.00%, 233.00% respectively. There were significant differences between the corresponding groups of hippocampus and cerebral cortex (P < 0.01).


CONCLUSIONS
No obvious change in mitochondria was found after 3 mW/cm(2) microwave irradiation, but it was found after 30 mW/cm(2) microwave irradiation. Mitochondria injury in cerebral cortex was more severe than that in hippocampus. mtTFA mRNA may have certain regulation in mitochondrial energy metabolism.
Xie
Yan
Y
Department of Military Labor Hygiene, Third Military Medical University, Chongqing 400038, China.
Jiang
Hai-hong
HH
Gong
Qian-fen
QF
Zhang
Guang-bin
GB
Yu
Jian-hua
JH
Yu
Zheng-ping
ZP
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000235:genetics*; D006624:Hippocampus / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D008854:Microscopy, Electron; D008872:Microwaves / Q000009:adverse effects*; D008928:Mitochondria / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure*; D024101:Mitochondrial Proteins / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000077278:RNA, Mitochondrial; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D024101:Mitochondrial Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D000077278:RNA, Mitochondrial; C489030:Tfam protein, rat; D014157:Transcription Factors; C422562:mitochondrial transcription factor A; D012313:RNA