title: Identification of the major vanilloid component in Capsicum extract by HPLC-EC and HPLC-MS.
abstract: A sensitive multi-channel HPLC-electrochemical (EC) method has been developed to determine the vanilloid content in the complex Capsicum annuum extract Capsibiol. Chromatographic separation was achieved within 10 min using a YMC Basic S5 column with a mobile phase containing chloroacetic acid, heptane sulphonic acid and acetonitrile. The multi-channel detector simultaneously applied four potentials between +500 and +800 mV (referenced to a silver/silver chloride electrode) to four glassy carbon working electrodes. The most abundant (0.94 mg/g) vanilloid analogue in the Capsibiol sample demonstrated an electrochemical reactivity and retention time similar to that of vanillic acid in HPLC-EC analysis. Its identity was confirmed by HPLC-MS using a Zorbax SB-CN column with a mobile phase containing formic acid and methanol.
authors.affiliation: Bioanalytical Systems Inc., 2701 Kent Avenue, West Lafayette, IN 47906, USA. qinz@bioanalytical.com
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhou
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Zhu
authors.forename: Hwa
authors.initials: H
authors.lastname: Chiang
authors.forename: Kader
authors.initials: K
authors.lastname: Yagiz
authors.forename: D James
authors.initials: DJ
authors.lastname: Morre
authors.forename: Dorothy M
authors.initials: DM
authors.lastname: Morre
authors.forename: Elsa
authors.initials: E
authors.lastname: Janle
authors.forename: Peter T
authors.initials: PT
authors.lastname: Kissinger
mesh_terms: D002212:Capsicum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014641:Vanillic Acid / Q000032:analysis* / Q000737:chemistry
chemical_list: C502157:Capsibiol; D010936:Plant Extracts; D014641:Vanillic Acid