Diagnostic multiplex PCR for toxin genotyping of Clostridium perfringens isolates.
In this study we provide a protocol for genotyping Clostridium perfringens with a new multiplex PCR. This PCR enables reliable and specific detection of the toxin genes cpa, cpb, etx, iap, cpe and cpb2 from heat lysed bacterial suspensions. The efficiency of the protocol was demonstrated by typing C. perfringens reference strains and isolates from veterinary bacteriological routine diagnostic specimens.
Baums
Christoph G
CG
Institut für Mikrobiologie und Tierseuchen, Tierärztliche Hochschule Hannover, Bischofsholer Damm 15, D-30173 Hannover, Germany.
Schotte
Ulrich
U
Amtsberg
Gunter
G
Goethe
Ralph
R
D000818:Animals; D003015:Clostridium Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary; D003016:Clostridium perfringens / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D007410:Intestinal Diseases / Q000382:microbiology / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000379:methods*
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes