Establishment and characterization of a renal cell carcinoma cell line (FU-UR-1) with the reciprocal ASPL-TFE3 fusion transcript.
We have established a cultured cell line named FU-UR-1 from a large retroperitoneal tumor of a 24-year-old Japanese male patient who simultaneously had a small renal cell carcinoma (RCC). Cytogenetic analysis and fluorescence in situ hybridization of the retroperitoneal tumor, and the cell line established from this tumor demonstrated similar karyotypes including add(13)(p11).ish der(13)t(13;17) (p11;q11)t(X;17)(p11;q25)(wcpX+,wcp17+). FU-UR-1 had been propagated continuously for more than 70 passages, and the doubling time was 32 h. Successful heterotransplantation was performed by inoculation of the cultured FU-UR-1 cells into the subcutis of BALB/c nude mice. Reverse transcriptase-polymerase chain reaction (RT-PCR) and sequence analysis demonstrated reciprocal ASPL-TFE3 and TFE3-ASPL fusion transcripts in the retroperitoneal tumor, cultured FU-UR-1 cells and xenografted tumors. In addition, the pathological findings of these samples and the renal tumor resembled each other. These observations suggest that the FU-UR-1 cell line established from the retroperitoneal tumor is an RCC cell line. This well-examined cell line may become a useful system for studying the genetic and biologic characteristics of rare neoplasms with the reciprocal ASPL-TFE3 fusion transcript.
Ishiguro
Masako
M
Department of Pathology, Fukuoka University School of Medicine, Jonan-ku, Fukuoka 814-0180, Japan. sbmasa@anet.ne.jp
Iwasaki
Hiroshi
H
Ohjimi
Yuko
Y
Kaneko
Yasuhiko
Y
D000328:Adult; D000818:Animals; D019677:Artificial Gene Fusion; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002455:Cell Division; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D047908:Intracellular Signaling Peptides and Proteins; D007621:Karyotyping; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000235:genetics*; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*; D014178:Translocation, Genetic; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
C422743:ASPSCR1 protein, human; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins; D015514:Oncogene Proteins, Fusion; D011993:Recombinant Fusion Proteins; C069203:TFE3 protein, human; D014157:Transcription Factors