Anemia with chronic renal disorder and disrupted metabolism of erythropoietin in ICR-derived glomerulonephritis (ICGN) mice.
The ICR-derived glomerulonephritis (ICGN) mouse, a new inbred mouse strain with a hereditary nephrotic syndrome, is considered to be a good model of human idiopathic nephrotic syndrome and notably exhibits proteinuria and hypoproteinemia from the neonatal stage. In chronic renal disorder (CRD), anemia is a major subsequent symptom (renal anemia). The precise cause of renal anemia remains unclear, primarily owing to the lack of appropriate spontaneous animal models for CRD. To establish adequate animal models for anemia with CRD, we examined the hematological-biochemical properties and histopathological characteristics. With the deterioration of renal function, ICGN mice developed a normochromic and normocytic anemia, and exhibited normochromic and microcytic at the terminal stage. The expression of erythropoietin (EPO) mRNA both in the kidneys and liver and the EPO leak into the urine were observed in ICGN mice, indicating a disrupted metabolism of EPO in ICGN mice. In addition, a lack of iron induced by the hemolysis in the spleen and the leak of transferrin into urine as proteinuria aggravated the anemic condition. In conclusion, the ICGN mouse is a good model for anemia with CRD.
Yamaguchi-Yamada
Misuzu
M
Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University, Japan.
Manabe
Noboru
N
Uchio-Yamada
Kozue
K
Akashi
Naotsugu
N
Goto
Yasufumi
Y
Miyamoto
Youhei
Y
Nagao
Masaya
M
Yamamoto
Yoshie
Y
Ogura
Atsuo
A
Miyamoto
Hajime
H
D000704:Analysis of Variance; D000740:Anemia / Q000150:complications*; D000818:Animals; D003433:Crosses, Genetic; D004195:Disease Models, Animal; D004921:Erythropoietin / Q000378:metabolism* / Q000652:urine; D005921:Glomerulonephritis / Q000150:complications*; D006403:Hematologic Tests; D006652:Histological Techniques; D007668:Kidney / Q000378:metabolism; D007676:Kidney Failure, Chronic / Q000150:complications*; D008099:Liver / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D004921:Erythropoietin