Genome sequence of the lignocellulose degrading fungus Phanerochaete chrysosporium strain RP78.
White rot fungi efficiently degrade lignin, a complex aromatic polymer in wood that is among the most abundant natural materials on earth. These fungi use extracellular oxidative enzymes that are also able to transform related aromatic compounds found in explosive contaminants, pesticides and toxic waste. We have sequenced the 30-million base-pair genome of Phanerochaete chrysosporium strain RP78 using a whole genome shotgun approach. The P. chrysosporium genome reveals an impressive array of genes encoding secreted oxidases, peroxidases and hydrolytic enzymes that cooperate in wood decay. Analysis of the genome data will enhance our understanding of lignocellulose degradation, a pivotal process in the global carbon cycle, and provide a framework for further development of bioprocesses for biomass utilization, organopollutant degradation and fiber bleaching. This genome provides a high quality draft sequence of a basidiomycete, a major fungal phylum that includes important plant and animal pathogens.
Martinez
Diego
D
US DoE Joint Genome Institute, Walnut Creek, California 94598, USA.
Larrondo
Luis F
LF
Putnam
Nik
N
Gelpke
Maarten D Sollewijn
MD
Huang
Katherine
K
Chapman
Jarrod
J
Helfenbein
Kevin G
KG
Ramaiya
Preethi
P
Detter
J Chris
JC
Larimer
Frank
F
Coutinho
Pedro M
PM
Henrissat
Bernard
B
Berka
Randy
R
Cullen
Dan
D
Rokhsar
Daniel
D
D001482:Base Composition / Q000235:genetics; D001673:Biodegradation, Environmental; D002482:Cellulose / Q000378:metabolism*; D002965:Classification; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D005091:Exons / Q000235:genetics; D005656:Fungal Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D015723:Gene Library; D005800:Genes, Fungal / Q000235:genetics; D016681:Genome, Fungal; D023281:Genomics; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism; D007438:Introns / Q000235:genetics; D042845:Laccase / Q000235:genetics / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism; D020075:Phanerochaete / Q000235:genetics* / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D018626:Retroelements / Q000235:genetics; D017422:Sequence Analysis, DNA; D019895:Transposases / Q000235:genetics
D004271:DNA, Fungal; D005656:Fungal Proteins; D011134:Polysaccharides; D018626:Retroelements; C036909:lignocellulose; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D042845:Laccase; D010544:Peroxidases; D016695:Glycosyltransferases; D019895:Transposases; D006026:Glycoside Hydrolases