title: Mass spectral analysis in proteomics.
abstract: Mass spectrometry provides key tools for the analysis of proteins. New types of mass spectrometers that provide enhanced capability to discover protein identities and perform improved proteomic experiments are discussed. Handling the complex mixtures of peptides and proteins generated from protein complexes and whole cells requires multidimensional separations; several forms of separation are discussed. Applications of mass spectrometry-based approaches for contemporary proteomic analyses are described.
authors.affiliation: Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA. jyates@scripps.edu
authors.forename: John R
authors.initials: JR
authors.lastname: Yates
mesh_terms: D002845:Chromatography / Q000379:methods* / Q000639:trends; D045424:Complex Mixtures / Q000032:analysis / Q000737:chemistry; D004586:Electrophoresis / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods* / Q000639:trends; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D020543:Proteome / Q000032:analysis* / Q000737:chemistry*; D040901:Proteomics / Q000379:methods* / Q000639:trends
chemical_list: D045424:Complex Mixtures; D011506:Proteins; D020543:Proteome