[Preparation and simultaneous determination of corynoline and acetylcorynoline in the herb of Corydalis bungeana].
OBJECTIVE
To isolate and purify corynoline and acetylcorynoline from Corydalis bungeana and develop a reversed-phase HPLC method of determining the two components in C. bungeana.


METHODS
Alkaloids were isolated from the ethanolic extract with column gel chromatography, and identified on the basis of spectral analysis (UV, 1H-NMR, 13C-NMR) and physicochemical properties. For quantitative analysis of the two components, samples were separated on an ODS column with mobile phase of methanol-15 mmol.L-1 potassium dihydrogen phosphate/potassium phosphate dibasic (pH 6.70, 70:30). The flow rate was 0.8 mL.min-1, and the detection was set at 289 nm.


RESULTS
The purity was 99.5% and 99.1% for corynoline and acetylcorynoline respectively. The calibration curves were linear in the range of 6.9-110.4 mg.L-1 corynoline and 8.7-139.5 mg.L-1 acetylcorynoline. The RSD was 2.1% and 2.7%, and the average recovery was 97.3% and 97.2% respectively.


CONCLUSIONS
The method of isolating and purifying corynoline and acetylcorynoline from Corydalis bungeana and the HPLC method of simultaneous determination of the two components have been developed. The HPLC method is simple, easy to perform and applicable to the content determination of corynoline and acetylcorynoline in C. bungeana of various origins.
Huang
Ge
G
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, Liaoning, China.
Yang
Hong-juan
HJ
Li
Fa-mei
FM
D001600:Berberine Alkaloids / Q000032:analysis / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D031311:Corydalis / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*
D001600:Berberine Alkaloids; C487645:acetylcorynoline; C487644:corynoline