title: Synthesis of fluorescein labeled 7-methylguanosinemonophosphate.
abstract: Binding of eIF4E to the cap structure (m(7)GpppN) plays a critical role in mRNA translation. To study the interaction between eIF4E and cap, and to identify small molecule inhibitors of their binding, we synthesized a fluorescent-labeled cap analogue and used it to develop a fluorescence-polarization assay. This preliminary communication describes the synthesis of a fluorescein labeled 7-methylguanosinemonophosphate, and its dose dependent binding to purified human eIF4E as demonstrated by the fluorescence polarization assay.
authors.affiliation: Laboratory for Translational Research, One Kendall Square, Building 600, 3rd Floor, Harvard Medical School, Cambridge, MA 02139, USA.
authors.forename: Amarnath
authors.initials: A
authors.lastname: Natarajan
authors.forename: Nathan
authors.initials: N
authors.lastname: Moerke
authors.forename: Yun-Hua
authors.initials: YH
authors.lastname: Fan
authors.forename: Han
authors.initials: H
authors.lastname: Chen
authors.forename: William J
authors.initials: WJ
authors.lastname: Christ
authors.forename: Gerhard
authors.initials: G
authors.lastname: Wagner
authors.forename: Jose A
authors.initials: JA
authors.lastname: Halperin
mesh_terms: D039561:Eukaryotic Initiation Factor-4E / Q000737:chemistry; D019793:Fluorescein; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes / Q000138:chemical synthesis; D006801:Humans; D011485:Protein Binding; D014176:Protein Biosynthesis; D012314:RNA Cap Analogs / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D039561:Eukaryotic Initiation Factor-4E; D005456:Fluorescent Dyes; D012314:RNA Cap Analogs; C011925:7-methylguanosine-5'-monophosphate; D019793:Fluorescein