A von Willebrand factor-binding protein from Staphylococcus lugdunensis.
In the present study, a phage display library covering the genome of Staphylococcus lugdunensis, was affinity-selected against von Willebrand factor (vWf). This led to the identification of a gene, vwbl, encoding a putative cell surface protein of 2060 amino acids, denoted vWbl. The deduced protein has an overall organisation typical of staphylococcal cell surface proteins, with an N-terminal signal peptide, and a C-terminal cell wall sorting signal. The vWf-binding part is located in repetitive domains and antibodies against vWbl or vWf can inhibit the binding. Southern blot analysis showed that vwbl was present in the 12 S. lugdunensis strains tested.
Nilsson
Martin
M
Department of Microbiology, Swedish University of Agricultural Sciences, SE75007 Uppsala, Sweden.
Bjerketorp
Joakim
J
Wiebensjö
Asa
A
Ljungh
Asa
A
Frykberg
Lars
L
Guss
Bengt
B
D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D015139:Blotting, Southern; D003001:Cloning, Molecular; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D015698:Genomic Library; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D019151:Peptide Library; D011485:Protein Binding; D021382:Protein Sorting Signals; D017434:Protein Structure, Tertiary; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D013210:Staphylococcus / Q000235:genetics* / Q000378:metabolism*; D014841:von Willebrand Factor / Q000378:metabolism*
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008565:Membrane Proteins; D019151:Peptide Library; D021382:Protein Sorting Signals; D014841:von Willebrand Factor