Enhanced resistance to blast fungus and bacterial blight in transgenic rice constitutively expressing OsSBP, a rice homologue of mammalian selenium-binding proteins.
The rice Oryza sativa selenium-binding protein homologue (OsSBP) gene encodes a homologue of mammalian selenium-binding proteins, and it has been isolated as one of the genes induced by treating a plant with a cerebroside elicitor from rice blast fungus. The possible role of OsSBP in plant defense was evaluated by using a transgenic approach. Plants overexpressing OsSBP showed enhanced resistance to a virulent strain of rice blast fungus as well as to rice bacterial blight. The expression of defense-related genes and the accumulation of phytoalexin after infection by rice blast fungus were accelerated in the OsSBP overexpressors. A higher level of H(2)O(2) accumulation and reduced activity of such scavenging enzymes as ascorbate peroxidase and catalase were seen when the OsSBP-overexpressing plants were treated with the protein phosphatase 1 inhibitor, calyculin A. These results suggest that the upregulation of OsSBP expression conferred enhanced tolerance to different pathogens, possibly by increasing plant sensitivity to endogenous defense responses. Additionally, the OsSBP protein might have a role in modulating the defense mechanism to biotic stress in rice.
Sawada
Kazutoshi
K
Research Association for Biotechnology, Ikoma, Nara, Japan. ksawada@bs.aist-nara.ac.jp
Hasegawa
Morifumi
M
Tokuda
Lena
L
Kameyama
Junko
J
Kodama
Osamu
O
Kohchi
Takayuki
T
Yoshida
Kazuya
K
Shinmyo
Atsuhiko
A
D000818:Animals; D018407:Bacterial Physiological Phenomena; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D005658:Fungi / Q000502:physiology*; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors / Q000235:genetics; D006861:Hydrogen Peroxide / Q000378:metabolism; D008322:Mammals; D008387:Marine Toxins; D012275:Oryza / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D010080:Oxazoles / Q000494:pharmacology; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D010936:Plant Extracts / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D051137:Selenium-Binding Proteins; D012717:Sesquiterpenes; D013729:Terpenes; D000094162:Phytoalexins
D002352:Carrier Proteins; D008387:Marine Toxins; D010080:Oxazoles; D010936:Plant Extracts; D010940:Plant Proteins; D051137:Selenium-Binding Proteins; D012717:Sesquiterpenes; D013729:Terpenes; C053376:pathogenesis-related proteins, plant; C059041:calyculin A; D006861:Hydrogen Peroxide; D000094162:Phytoalexins