Immunodiagnosis of human fascioliasis using an antigen of Fasciola gigantica adult worm with the molecular mass of 27 kDa by a dot-ELISA.
Immunodominant antigens of an approximate molecular mass of 27 kDa (FG 27) were obtained from an excretory-secretory product of adult Fasciola gigantica by a simple continuous-elution method. A dot-ELISA using the FG 27 antigen was developed for the detection of specific antibodies from patients infected with F. gigantica. Control sera were obtained from patients with other parasitic infections and healthy volunteers. The accuracy, sensitivity, specificity, and positive and negative predictive values were 98.2%, 100%, 97.4%, 76.9% and 100%, respectively. This dot-ELISA is a specific, sensitive and easy to perform method for the rapid diagnosis of fascioliasis, particularly when more complex laboratory tests are unavailable.
Intapan
Pewpan M
PM
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. pewpan@kku.ac.th
Maleewong
Wanchai
W
Nateeworanart
Saengchai
S
Wongkham
Chaisiri
C
Pipitgool
Vichit
V
Sukolapong
Varaporn
V
Sangmaneedet
Somboon
S
D000818:Animals; D000947:Antigens, Helminth; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005209:Fasciola / Q000276:immunology*; D005211:Fascioliasis / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
D000947:Antigens, Helminth