title: The effects of phytase and phytic acid on the loss of endogenous amino acids and minerals from broiler chickens.
abstract: 1. The effects of myo-inositol hexaphosphate (IP6) and phytase (EC 3.1.3.26) on the excretion of endogenous compounds were investigated using growing broiler chickens. 2. A total of 32 female Ross broilers were used in a precision feeding assay involving a 2 x 2 factorial arrangement of treatments. The materials administered were glucose, glucose + 1000 units of phytase activity (FTU), glucose + 1 g of IP6 and glucose + 1 g of IP6 + 1000 FTU. Excreta were collected quantitatively over a 48-h period following intubation of the test materials. The excretion of nitrogen, amino acids, minerals, sialic acid and phytate phosphorus was determined. 3. The ingestion of 1 g of IP6 by broilers increased the excretion of endogenous nitrogen, amino acids, iron, sodium, sulphur and sialic acid compared with birds fed on glucose. Supplementation of IP6 with exogenous phytase reduced the excretion of endogenous amino acids, calcium, sodium, phytate phosphorus and sialic acid compared with birds fed IP6. 4. It can be concluded that IP6 increases the excretion of endogenous minerals and amino acids in broiler chickens. Part of the beneficial effects of the addition of exogenous phytases to the diets of poultry appears to be mediated through a reduction in endogenous losses of these nutrients.
authors.affiliation: Avian Science Research Centre, Scottish Agricultural College, Ayr, Scotland.
authors.forename: A J
authors.initials: AJ
authors.lastname: Cowieson
authors.forename: T
authors.initials: T
authors.lastname: Acamovic
authors.forename: M R
authors.initials: MR
authors.lastname: Bedford
mesh_terms: D010832:6-Phytase / Q000378:metabolism / Q000494:pharmacology*; D000596:Amino Acids / Q000032:analysis / Q000378:metabolism*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D005243:Feces / Q000737:chemistry*; D005260:Female; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism; D019158:N-Acetylneuraminic Acid / Q000032:analysis; D009584:Nitrogen / Q000032:analysis; D010758:Phosphorus / Q000032:analysis; D010833:Phytic Acid / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D011897:Random Allocation
chemical_list: D000596:Amino Acids; D010758:Phosphorus; D010833:Phytic Acid; D010832:6-Phytase; D019158:N-Acetylneuraminic Acid; D005947:Glucose; D009584:Nitrogen