Fungal laccase-catalyzed degradation of hydroxy polychlorinated biphenyls.
Hydroxy polychlorinated biphenyls (hydroxy PCBs) are toxic metabolites of PCBs. Their toxicity such as strong endocrine disruption demands effective remediation methods. Laccases from Trametes versicolor and Pleurotus ostreatus were tested to degrade hydroxy PCBs. Optimum pHs for both enzymes were around 4.0. Laccase from T. versicolor degrades hydroxy PCBs more rapidly than that from P. ostreatus. The enzymatic activities remained little changes in up to 10% organic solvents, but decreased rapidly in more than 10% acetone, acetonitrile or DMSO. Degradation rate constants decreased with increase of chlorination and no degradation was observed with tetra-, penta- and hexa-chloro hydroxy PCBs in non-mediated reactions. However, the tetra- to hexa-chloro hydroxy PCBs were degraded by laccase from T. versicolor in the presence of the mediator 2,2,6,6-tetramethylpiperidine-N-oxy radical. The other mediators, 4-ethyl-2-methoxyphenol, 2,2'-azino-bis(3-ethylbenzthiazoline sulfonic acid) diammonium salt and 1-hydroxybenzotriazole and humic acid, also enhanced degradation of all the hydroxy PCBs except 4-hydroxy-2',3,3',4',5,5'-hexachlorobiphenyl. The results showed that 3-hydroxy biphenyl was more resistant to laccase degradation than 2- or 4-hydroxy analogues. Significant linear-correlations (coefficient of determination, r2 = 0.9097 and 0.8186 for laccases from P. ostreatus and T. versicolor, respectively) were found between the ionization potentials and the removal rate constants of hydroxy PCBs.
Keum
Young Soo
YS
Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, 1955 East-West Road, Ag Sci 218, Honolulu, HI 96822, USA.
Li
Qing X
QX
D000096:Acetone; D000097:Acetonitriles; D001487:Basidiomycota / Q000201:enzymology*; D001673:Biodegradation, Environmental; D002384:Catalysis; D004121:Dimethyl Sulfoxide; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D042845:Laccase / Q000378:metabolism*; D008958:Models, Molecular; D011078:Polychlorinated Biphenyls / Q000378:metabolism*; D012997:Solvents
D000097:Acetonitriles; D012997:Solvents; D000096:Acetone; D011078:Polychlorinated Biphenyls; D042845:Laccase; D004121:Dimethyl Sulfoxide; C032159:acetonitrile