Tensile strength and microhardness of treated human dentin.
OBJECTIVE
To determine the ultimate tensile strength and Knoop hardness of mineralized, EDTA-treated, sodium hypochlorite (NaOCl)-treated, EDTA-treated resin-infiltrated, and NaOCl-treated resin-infiltrated dentin.


METHODS
Dumbell-shaped specimens with a cross-sectional area of 0.5 mm2 were prepared from the crowns of extracted human third molars. Specimens were randomly assigned to the following experimental groups: (1) mineralized dentin; (2) 0.5 M EDTA-demineralized dentin, pH 7/5 days; (3) 5% NaOCl-deproteinized dentin/2 days; (4) EDTA-treated, Single Bond resin-infiltrated dentin; (5) NaOCl-treated, Single Bond resin-infiltrated dentin. All specimens were tested in tension in a Vitrodyne testing machine at 0.6 mm/min. Knoop microhardness was measured on the fractured edges of specimens in groups 1, 3, 4, and 5. Results were analyzed by ANOVA and SNK tests (p < 0.05).


RESULTS
Both EDTA and NaOCl treatments caused significant reductions in the tensile strength and microhardness of mineralized dentin (p < 0.05) with the largest reductions observed after NaOCl treatment (p < 0.05) Resin infiltration of treated dentin resulted in moderate increase of its tensile strength and microhardness, however, the original mineralized values were not recovered (p < 0.05).


CONCLUSIONS
Whenever dentin surfaces are treated with EDTA or NaOCl prior to a clinical bonding procedure, clinicians must be aware that a weak layer may be present at the interface, which may lead to premature failures of resin/dentin bonds.
Fuentes
Victoria
V
Department of Dental Materials, School of Dentistry Granada, University of Granada, Spain.
Ceballos
Laura
L
Osorio
Raquel
R
Toledano
Manuel
M
Carvalho
Ricardo M
RM
Pashley
David H
DH
D000704:Analysis of Variance; D017438:Bisphenol A-Glycidyl Methacrylate / Q000493:pharmacokinetics; D001840:Dental Bonding; D003799:Dental Stress Analysis; D003804:Dentin / Q000187:drug effects* / Q000378:metabolism; D017220:Dentin-Bonding Agents / Q000493:pharmacokinetics; D004492:Edetic Acid / Q000494:pharmacology; D006244:Hardness; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000493:pharmacokinetics*; D012973:Sodium Hypochlorite / Q000494:pharmacology; D018709:Statistics, Nonparametric; D013718:Tensile Strength; D017001:Tooth Demineralization
D017220:Dentin-Bonding Agents; D008689:Methacrylates; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate; C005044:hydroxyethyl methacrylate; D004492:Edetic Acid; D012973:Sodium Hypochlorite