title: Functional expression of the ectoine hydroxylase gene (thpD) from Streptomyces chrysomallus in Halomonas elongata.
abstract: The formation of hydroxyectoine in the industrial ectoine producer Halomonas elongata was improved by the heterologous expression of the ectoine hydroxylase gene, thpD, from Streptomyces chrysomallus. The efficient conversion of ectoine to hydroxyectoine was achieved by the concerted regulation of thpD by the H. elongata ectA promoter.
authors.affiliation: ActinoDrug Pharmaceuticals GmbH, 16761 Hennigsdorf, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Prabhu
authors.forename: Florian
authors.initials: F
authors.lastname: Schauwecker
authors.forename: Nicolas
authors.initials: N
authors.lastname: Grammel
authors.forename: Ullrich
authors.initials: U
authors.lastname: Keller
authors.forename: Michael
authors.initials: M
authors.lastname: Bernhard
mesh_terms: D000599:Amino Acids, Diamino / Q000096:biosynthesis / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D015964:Gene Expression Regulation, Bacterial; D020632:Halomonas / Q000201:enzymology* / Q000235:genetics; D006899:Mixed Function Oxygenases / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics
chemical_list: D000599:Amino Acids, Diamino; D001426:Bacterial Proteins; C121557:hydroxyectoine; C045628:ectoine; D006899:Mixed Function Oxygenases