title: Expression profiling of purified normal human luminal and myoepithelial breast cells: identification of novel prognostic markers for breast cancer.
abstract: The normal duct-lobular system of the breast is lined by two epithelial cell types, inner luminal secretory cells and outer contractile myoepithelial cells. We have generated comprehensive expression profiles of the two normal cell types, using immunomagnetic cell separation and gene expression microarray analysis. The cell-type specificity was confirmed at the protein level by immunohistochemistry in normal breast tissue. New prognostic markers for survival were identified when the luminal- and myoepithelial-specific molecules were evaluated on breast tumor tissue microarrays. Nuclear expression of luminal epithelial marker galectin 3 correlated with a shorter overall survival in these patients, and the expression of SPARC (osteonectin), a myoepithelial marker, was an independent marker of poor prognosis in breast cancers as a whole. These data provide a framework for the interpretation of breast cancer molecular profiling experiments, the identification of potential new diagnostic markers, and development of novel indicators of prognosis.
authors.affiliation: The Breakthrough Toby Robins Breast Cancer Research Centre, Institute of Cancer Research, London, United Kingdom.
authors.forename: Chris
authors.initials: C
authors.lastname: Jones
authors.forename: Alan
authors.initials: A
authors.lastname: Mackay
authors.forename: Anita
authors.initials: A
authors.lastname: Grigoriadis
authors.forename: Antonio
authors.initials: A
authors.lastname: Cossu
authors.forename: Jorge S
authors.initials: JS
authors.lastname: Reis-Filho
authors.forename: Laura
authors.initials: L
authors.lastname: Fulford
authors.forename: Tim
authors.initials: T
authors.lastname: Dexter
authors.forename: Susan
authors.initials: S
authors.lastname: Davies
authors.forename: Karen
authors.initials: K
authors.lastname: Bulmer
authors.forename: Emily
authors.initials: E
authors.lastname: Ford
authors.forename: Suzanne
authors.initials: S
authors.lastname: Parry
authors.forename: Mario
authors.initials: M
authors.lastname: Budroni
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Palmieri
authors.forename: A Munro
authors.initials: AM
authors.lastname: Neville
authors.forename: Michael J
authors.initials: MJ
authors.lastname: O'Hare
authors.forename: Sunil R
authors.initials: SR
authors.lastname: Lakhani
mesh_terms: D001940:Breast / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D015676:Osteonectin / Q000378:metabolism; D011379:Prognosis
chemical_list: D015676:Osteonectin