Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli.
The gene encoding the excretory-secretory antigen TES-120 of dog ascarid worm Toxocara canis was cloned into the bacterium Escherichia coli. The specificity of the recombinant TES-120 antigen produced by the bacterium was investigated. A total of 45 human serum samples from patients infected with differenthelminthes and protozoa, including 8 cases of toxocariasis, were tested against the recombinant antigens in immunoblot assays. The results from the assays revealed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis.
Fong
Mun-Yik
MY
Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. fongmy@um.edu.my
Lau
Yee-Ling
YL
Init
Ithoi
I
Jamaiah
I
I
Anuar
A Khairul
AK
Rahmah
N
N
D000818:Animals; D000947:Antigens, Helminth / Q000378:metabolism; D003001:Cloning, Molecular; D004285:Dogs; D004926:Escherichia coli / Q000378:metabolism; D015801:Helminth Proteins / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D007815:Larva Migrans / Q000175:diagnosis* / Q000276:immunology; D011994:Recombinant Proteins / Q000378:metabolism; D012680:Sensitivity and Specificity; D017166:Toxocara canis / Q000276:immunology*
D000947:Antigens, Helminth; D015801:Helminth Proteins; D011994:Recombinant Proteins; C045159:TES-26 protein, Toxocara canis