Testing of newly developed glycophospholipid antigen for the detection of P. falciparum malaria by laser light immunoassay in endemic and non-endemic areas.
A glycophospholipid (GPL) antigen isolated from Plasmodium falciparum culture supernatant has been tested for its antigenicity. Detection of malaria positive known blood samples and unknown field samples from endemic and non-endemic areas were compared. In this study laser light scattering immunoassay (LIA) was used for the detection of P. falciparum malaria. Test results of control (malaria negative samples from Surat) were compared with known positive samples and unknown malaria positive field samples. A positive correlation has been observed (97%) in falciparum positive samples from laboratory and unknown samples from endemic area (Haldwani) by LIA method using GPL antigen. From the results of the study it was found that GPL antigen has a better antigenic property and can detect almost all the cases of Pf malaria by LIA method.
Roy
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A
Malaria Research Centre (ICMR), 22 Sham Nath Marg, Delhi, India.
Biswas
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S
Mya
M M
MM
Saxena
R K
RK
Roy
K B
KB
D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan; D019353:Endemic Diseases; D005260:Female; D006801:Humans; D007118:Immunoassay; D007834:Lasers; D016778:Malaria, Falciparum / Q000097:blood / Q000469:parasitology*; D008297:Male; D010743:Phospholipids; D010963:Plasmodium falciparum / Q000276:immunology / Q000302:isolation & purification*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D010743:Phospholipids