title: mRNA expression profiling reveals a role of Helicobacter pylori vacuolating toxin in escaping host defense.
abstract: OBJECTIVE
  To study the immune response of host to Helicobacter pylori VacA.
  
  
  METHODS
  The monocyte/macrophage-like U937 cells were infected with Helicobacter pylori vacA-positive strain NCTC 11638 or isogenic vacA-negative mutant. Differentially expressed genes were identified at 2, 6, 10, and 24 h post-infection by cDNA microarray. Differential expressions of some genes were confirmed by Northern blot.
  
  
  RESULTS
  More than 100 genes altered their mRNA expression at different time points respectively, many of which were identified to be related to immune evasion.
  
  
  CONCLUSIONS
  VacA is a crucial element for H pylori to escape from host immune defense by means of differentially regulating the expression of some related genes. These genes, previously known or unknown to be involved in the mechanism of immune evasion, deserve further investigation to unearth much more information complicated in the immune response.
authors.affiliation: Department of Microbiology and Parasitology, Shanghai Second Medical University, 280 Chongqingnan Road, Shanghai 200025, China.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Yuan
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Zhen-Hong
authors.initials: ZH
authors.lastname: Li
authors.forename: Gui-Zhen
authors.initials: GZ
authors.lastname: Yang
authors.forename: Bao-Yu
authors.initials: BY
authors.lastname: Hu
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Shi
authors.forename: Tie-Liu
authors.initials: TL
authors.lastname: Shi
authors.forename: Shan-Qing
authors.initials: SQ
authors.lastname: Tong
authors.forename: Xiao-Kui
authors.initials: XK
authors.lastname: Guo
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D016480:Helicobacter pylori / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D007107:Immune System / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D012333:RNA, Messenger; C074689:VacA protein, Helicobacter pylori