Preimplantation genetic diagnosis for the treatment of failed in vitro fertilization-embryo transfer and habitual abortion.
OBJECTIVE
To apply preimplantation genetic diagnosis (PGD) for the treatment of patients with a history of failed IVF-ET or habitual aborters.


METHODS
Prospective clinical study.


METHODS
Tertiary center for assisted reproduction.


METHODS
Ninety-four couples with failed IVF-ET after >2 IVF cycles and 64 couples with >2 spontaneous abortions.


METHODS
Patients were prepared for oocyte retrieval using standard controlled ovarian hyperstimulation protocols after standard laboratory techniques. Blastomeres from 6- to 8-cell embryos were analysed using fluorescence in situ hybridization with commercial chromosomal probes, and normoploid embryos were transferred on day 3 after fertilization.


METHODS
Pregnancy and implantation rates and live births.


RESULTS
Both 3- and 5-probe PGD resulted in a significantly higher outcome than controls for failed IVF-ET. Five-probe PGD appeared to be more suitable for habitual aborters.


CONCLUSIONS
This pilot study suggests that 3-probe PGD is a valid option for failed IVF-ET patients. The use of five or more probes is indicated for habitual aborters.
Wilding
Martin
M
Center for Reproductive Biology, Clinica Villa del Sole, Naples, Italy.
Forman
Robert
R
Hogewind
George
G
Di Matteo
Loredana
L
Zullo
Fulvio
F
Cappiello
Fulvio
F
Dale
Brian
B
D000026:Abortion, Habitual / Q000235:genetics*; D000328:Adult; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D011247:Pregnancy; D019836:Preimplantation Diagnosis; D011446:Prospective Studies