title: Hierarchically biomimetic bone scaffold materials: nano-HA/collagen/PLA composite.
abstract: A bone scaffold material (nano-HA/ collagen/PLA composite) was developed by biomimetic synthesis. It shows some features of natural bone both in main composition and hierarchical microstructure. Nano-hydroxyapatite and collagen assembled into mineralized fibril. The three-dimensional porous scaffold materials mimic the microstructure of cancellous bone. Cell culture and animal model tests showed that the composite material is bioactive. The osteoblasts were separated from the neonatal rat calvaria. Osteoblasts adhered, spread, and proliferated throughout the pores of the scaffold material within a week. A 15-mm segmental defect model in the radius of the rabbit was used to evaluate the bone-remodeling ability of the composite. Combined with 0.5 mg rhBMP-2, the material block was implanted into the defect. The segmental defect was integrated 12 weeks after surgery, and the implanted composite was partially substituted by new bone tissue. This scaffold composite has promise for the clinical repair of large bony defects according to the principles of bone tissue engineering.
authors.affiliation: Biomaterials Laboratory, Department of Material Science & Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
authors.forename: S S
authors.initials: SS
authors.lastname: Liao
authors.forename: F Z
authors.initials: FZ
authors.lastname: Cui
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: Q L
authors.initials: QL
authors.lastname: Feng
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D040761:Biomimetic Materials; D001861:Bone Regeneration; D016723:Bone Remodeling; D018786:Bone Substitutes; D001842:Bone and Bones / Q000502:physiology*; D002478:Cells, Cultured; D003094:Collagen; D019245:Compressive Strength; D017886:Durapatite; D020007:Implants, Experimental; D019344:Lactic Acid; D008422:Materials Testing; D008854:Microscopy, Electron; D016348:Osseointegration; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D011091:Polyesters; D011108:Polymers; D011817:Rabbits; D051381:Rats; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen; D017886:Durapatite