title: Evolution of expression of cardiac phenotypes over a 4-year period in the beta-myosin heavy chain-Q403 transgenic rabbit model of human hypertrophic cardiomyopathy.
abstract: Hypertrophic cardiomyopathy (HCM), the most common cause of sudden cardiac death in the young, is characterized by a diverse array of cardiac phenotypes evolving over several decades. We have developed transgenic rabbits that fully recapitulate the phenotype of human HCM and provide for the opportunity to delineate the sequence of evolution of cardiac phenotypes, and thus, the pathogenesis of HCM. We determined evolution of biochemical, molecular, histological, structural and functional phenotypes at 4 age-periods in 47 beta-myosin heavy chain-glutamine (MyHC-Q)-403 transgenic rabbits. Ca(+2) sensitivity of myofibrillar ATPase activity was reduced very early and in the absence of other discernible phenotypes. Myocyte disarray also occurred early, prior to, and independent of hypertrophy and fibrosis. The latter phenotypes evolved predominantly during puberty in conjunction with activation of stress-related signaling kinases. Myocardial contraction and relaxation velocities were decreased early despite normal global cardiac function and in the absence of histological phenotype. Global cardiac function declined with aging, while left atrial size was increased along with Doppler indices of left ventricular filling pressure. Thus, Ca(+2) sensitivity of myofibrillar ATPase activity is a primary phenotype expressed early and independent of the ensuing phenotypes. Pathogenesis of myocyte disarray, which exhibits age-independent penetrance, differs from those of hypertrophy and fibrosis, which show age-dependent expression. Myocardial dysfunction is an early marker that predicts subsequent development of hypertrophy. These findings in an animal model that recapitulates the phenotype of human HCM, implicate involvement of multiple independent mechanisms in the pathogenesis of cardiac phenotypes in HCM.
authors.affiliation: Sections of Cardiology, Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Sherif F
authors.initials: SF
authors.lastname: Nagueh
authors.forename: Suetnee
authors.initials: S
authors.lastname: Chen
authors.forename: Rajnikant
authors.initials: R
authors.lastname: Patel
authors.forename: Natalia
authors.initials: N
authors.lastname: Tsybouleva
authors.forename: Silvia
authors.initials: S
authors.lastname: Lutucuta
authors.forename: Helen A
authors.initials: HA
authors.lastname: Kopelen
authors.forename: William A
authors.initials: WA
authors.lastname: Zoghbi
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Quiñones
authors.forename: Robert
authors.initials: R
authors.lastname: Roberts
authors.forename: A J
authors.initials: AJ
authors.lastname: Marian
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000375:Aging / Q000502:physiology; D000818:Animals; D030801:Animals, Genetically Modified; D002118:Calcium / Q000378:metabolism; D002312:Cardiomyopathy, Hypertrophic / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D004195:Disease Models, Animal; D004452:Echocardiography; D005973:Glutamine / Q000235:genetics / Q000378:metabolism; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D009200:Myocardial Contraction; D009210:Myofibrils / Q000187:drug effects / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000235:genetics* / Q000378:metabolism*; D024501:Nonmuscle Myosin Type IIB; D010641:Phenotype; D011817:Rabbits; D015398:Signal Transduction; D013997:Time Factors; D019076:Transgenes / Q000235:genetics*
chemical_list: D005973:Glutamine; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D000251:Adenosine Triphosphatases; D024501:Nonmuscle Myosin Type IIB; C103815:nonmuscle myosin type IIB heavy chain; D018995:Myosin Heavy Chains; D002118:Calcium