Biotic disturbance, recolonization, and early succession of bacterial assemblages in intertidal sediments.
The role of disturbance in structuring natural microbial communities has been largely unexplored. Disturbance associated with invertebrate ingestion can reduce bacterial biomass and alter metabolic activities and compositions of bacterial assemblages in marine sediments. The primary objectives of the research presented here were to test whether ingestion by a taxonomically diverse group of deposit feeders constituted a disturbance, and to determine the mechanisms by which bacterial assemblages recover following deposit-feeder ingestion. To test the question of disturbance, we compared fresh egesta vs surficial sediments with respect to bacterial assemblage structure. In emersed intertidal sediments, microbial recovery could be due to regrowth of bacterial populations surviving gut passage or to immigration from adjacent sediments. To differentiate between these modes of recolonization we used field manipulative experiments to exclude migration by isolating freshly extruded fecal coils of three deposit-feeding species from surrounding sediments. We then followed the quantitative and qualitative recovery in egesta and sediments through time using epifluorescence microscopy and PCR-DGGE analysis of 16S rDNA. Our findings indicate that (1) the degree and nature of the disturbance to bacterial assemblages from deposit feeding varies among invertebrate taxa, (2) recovery was significant but incomplete over 3 h, and (3) recolonization of biotically disturbed sediments is dominated by immigration.
Plante
C J
CJ
Department of Biology, Grice Marine Laboratory, College of Charleston, Charleston, SC 29412, USA. plantec@cofc.edu
Wilde
S B
SB
D000704:Analysis of Variance; D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics; D017753:Ecosystem; D004586:Electrophoresis; D005243:Feces / Q000737:chemistry; D005247:Feeding Behavior / Q000502:physiology*; D019015:Geologic Sediments / Q000382:microbiology*; D007448:Invertebrates / Q000502:physiology*; D008856:Microscopy, Fluorescence; D016133:Polymerase Chain Reaction; D011157:Population Dynamics; D013022:South Carolina
D017931:DNA Primers; D004275:DNA, Ribosomal