title: Evaluation of the card agglutination test (CATT/T. evansi) for detection of Trypanosoma evansi infection in water buffaloes (Bubalus bubalis) in Egypt.
abstract: A card agglutination test (CATT/T. evansi) was evaluated for detection of antibodies against Trypanosoma evansi (T. evansi) in experimentally and naturally infected buffaloes. Four calves were inoculated with a strain of T. evansi isolated from a dromedary camel. Parasitological examination of the calves revealed trypanosomes in the blood from days 4 to 9 post-inoculation (PI). General emaciation appeared from day 26 PI and aggravated until the end of the experiment (day 88 PI). Antibodies against T. evansi were detectable from day 8 PI till the end of the experiment. Parasitological examination of 200 water buffalo blood samples obtained from slaughterhouses revealed negative results. Serological examination of these animals showed that 48 (24%) water buffaloes had anti-T. evansi antibodies.
authors.affiliation: Parasitology Department, Faculty of Veterinary Medicine, Cairo University, P.O. Box 12211, Giza, Cairo, Egypt. mosaadhilai@yahoo.com
authors.forename: M
authors.initials: M
authors.lastname: Hilali
authors.forename: A
authors.initials: A
authors.lastname: Abdel-Gawad
authors.forename: A
authors.initials: A
authors.lastname: Nassar
authors.forename: A
authors.initials: A
authors.lastname: Abdel-Wahab
authors.forename: E
authors.initials: E
authors.lastname: Magnus
authors.forename: P
authors.initials: P
authors.lastname: Büscher
mesh_terms: D000372:Agglutination Tests / Q000379:methods / Q000592:standards / Q000662:veterinary*; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001831:Body Temperature; D001835:Body Weight; D002020:Buffaloes / Q000097:blood / Q000469:parasitology*; D004534:Egypt; D051379:Mice; D018512:Parasitemia; D014345:Trypanosoma / Q000302:isolation & purification*; D014352:Trypanosomiasis / Q000097:blood / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*
chemical_list: D000913:Antibodies, Protozoan