title: Genetic diversity and molecular phylogeny of Anaplasma marginale isolates from Minas Gerais, Brazil.
abstract: Anaplasma marginale (Rickettsiales: Anaplasmataceae), a tick-borne pathogen of cattle, is endemic in tropical and subtropical regions of the world, and many isolates of A. marginale may occur in a given geographic area. Phylogenetic relationships have been reported for A. marginale isolates from the US using gene and protein sequences of MSP1a and msp4. These studies demonstrated that msp4 sequences, but not MSP1a DNA or protein sequences, provide phylogeographic information and also that MSP1a sequences are highly heterogeneous among A. marginale populations. However, little information is available on the genetic diversity of A. marginale isolates from other regions of the world. The present study was undertaken to examine genetic variation among 10 isolates of A. marginale obtained from infected cattle in the State of Minas Gerais, Brazil, where A. marginale is endemic. Neighbor-joining analysis of msp4 sequences of Brazilian and New World isolates of A. marginale from Argentina, Mexico and the US provided bootstrap support for a Latin American clade. The sequences of the MSP1a repeats of four Brazilian isolates of A. marginale were compared to sequences of Latin American and US isolates. The MSP1a repeated sequences of Latin American isolates of A. marginale had nine repeat forms, alpha-phi, which have not been reported previously in North American isolates of A. marginale. Furthermore, the repeated forms tau, sigma and mu were only present in the Brazilian isolates. The results demonstrated that the genetic heterogeneity observed among isolates of A. marginale is common in endemic areas, independent of the predominant tick vector and is consistent with previous studies in which msp4 provided phylogeographic information about A. marginale isolates, while MSP1a was found not to be a useful marker for phylogeographic characterization of A. marginale isolates.
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, 250 McElroy Hall, Stillwater, OK 74078, USA. jose_delafuente@yahoo.com
authors.forename: José
authors.initials: J
authors.lastname: de La Fuente
authors.forename: Lygia M F
authors.initials: LM
authors.lastname: Passos
authors.forename: Ronald A
authors.initials: RA
authors.lastname: Van Den Bussche
authors.forename: Múcio F B
authors.initials: MF
authors.lastname: Ribeiro
authors.forename: E J
authors.initials: EJ
authors.lastname: Facury-Filho
authors.forename: Katherine M
authors.initials: KM
authors.lastname: Kocan
mesh_terms: D000595:Amino Acid Sequence; D042322:Anaplasma marginale / Q000235:genetics* / Q000302:isolation & purification; D000712:Anaplasmosis / Q000469:parasitology*; D000818:Animals; D001483:Base Sequence; D001938:Brazil; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment
chemical_list: D016054:DNA, Protozoan