Spinal neurons involved in the generation of at-level pain following spinal injury in the rat.
Using a conjugate of substance P and the ribosome-inactivating protein saporin, neurons expressing the neurokinin-1 receptor in lamina I of the spinal cord were targeted to determine their role in the expression of a spontaneous pain behavior following intraspinal injections of quisqualic acid in the rat. Treatment was carried out at the time of injury in order to prevent the onset of the behavior, and following onset in order to evaluate the potential clinical utility of this intervention. Treatment at the time of injury resulted in significant decreases in onset-time and severity of pain behavior, while treatment at the time of onset led to a significant reduction of the spontaneous self-directed behavior. The results suggest that the substrate for at-level pain following spinal cord injury includes a population of spinal neurons expressing the neurokinin-1 receptor in the superficial laminae of the spinal cord.
Yezierski
Robert P
RP
Department of Orthodontics, University of Florida, Gainesville, FL 32610, USA. ryezierski@dental.ufl.edu
Yu
Chen-Guang
CG
Mantyh
Patrick W
PW
Vierck
Charles J
CJ
Lappi
Douglas A
DA
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D004195:Disease Models, Animal; D006120:Grooming / Q000187:drug effects / Q000502:physiology; D007150:Immunohistochemistry; D000922:Immunotoxins / Q000494:pharmacology / Q000627:therapeutic use; D009699:N-Glycosyl Hydrolases / Q000494:pharmacology / Q000627:therapeutic use; D009498:Neurotoxins / Q000494:pharmacology* / Q000627:therapeutic use; D010146:Pain / Q000188:drug therapy / Q000209:etiology* / Q000503:physiopathology*; D010940:Plant Proteins / Q000494:pharmacology / Q000627:therapeutic use; D020671:Posterior Horn Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D018040:Receptors, Neurokinin-1 / Q000187:drug effects / Q000378:metabolism*; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins; D012652:Self Mutilation / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology; D012867:Skin / Q000294:innervation; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology*; D013119:Spinal Cord Injuries / Q000150:complications*; D013373:Substance P / Q000494:pharmacology / Q000627:therapeutic use
D000922:Immunotoxins; D009498:Neurotoxins; D010940:Plant Proteins; D018040:Receptors, Neurokinin-1; D054789:Ribosome Inactivating Proteins, Type 1; D013373:Substance P; D009699:N-Glycosyl Hydrolases; D000076985:Saporins