Characterization of an excreted/secreted antigen form of 14-3-3 protein in Toxoplasma gondii tachyzoites.
The 14-3-3 protein was shown to be present into the parasitophorous vacuole of Toxoplasma gondii-infected human monocyte cells and in the excreted/secreted antigens (ESA). The ESA 14-3-3 protein migrates electrophoretically as the cytosol and the main membranous 14-3-3 isoforms. The excretion/secretion of 14-3-3 was not sensitive to cycloheximide, a protein synthesis inhibitor, even at a concentration which inhibited the production of 14-3-3 inside the tachyzoites. Recombinant 14-3-3/GST protein was used to test the presence of 14-3-3 antibodies in different human sera. A positive immunoreactivity was observed with sera corresponding to acute toxoplasmosis and a possible involvement of 14-3-3 in host immunity is discussed.
Assossou
Olga
O
Laboratoire de Parasitologie, Mycologie Médicale et Pathologie Exotique, Equipe d'accueil 3087 Faculté de Médecine, Université Claude Bernard, 8 avenue Rockefeller, 69373 Lyon, France. oasso@rockefeller.univ-lyon1.fr
Besson
Françoise
F
Rouault
Jean-Pierre
JP
Persat
Florence
F
Ferrandiz
Josette
J
Mayençon
Martine
M
Peyron
François
F
Picot
Stèphane
S
D048948:14-3-3 Proteins; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D015870:Gene Expression; D006801:Humans; D007700:Kinetics; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D009000:Monocytes / Q000378:metabolism / Q000469:parasitology / Q000648:ultrastructure; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D015800:Protozoan Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000276:immunology; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000276:immunology* / Q000469:parasitology; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis / Q000276:immunology* / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism / Q000469:parasitology / Q000648:ultrastructure
D048948:14-3-3 Proteins; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D011500:Protein Synthesis Inhibitors; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; C408624:excretory-secretory antigen, Toxoplasma; D003513:Cycloheximide; D014446:Tyrosine 3-Monooxygenase