Global analysis of gene expression in an rpoN mutant of Listeria monocytogenes.
The role of the alternative sigma(54) factor, encoded by the rpoN gene, was investigated in Listeria monocytogenes by comparing the global gene expression of the wild-type EGDe strain and an rpoN mutant. Gene expression, using whole-genome macroarrays, and protein content, using two-dimensional gel electrophoresis, were analysed. Seventy-seven genes and nine proteins, whose expression was modulated in the rpoN mutant as compared to the wild-type strain, were identified. Most of the modifications were related to carbohydrate metabolism and in particular to pyruvate metabolism. However, under the conditions studied, only the mptACD operon was shown to be directly controlled by sigma(54). Therefore, the remaining modifications seem to be due to indirect effects. In parallel, an in silico analysis suggests that sigma(54) may directly control the expression of four different phosphotransferase system (PTS) operons, including mptACD. PTS activity is known to have a direct effect on the pyruvate pool and on catabolite regulation. These results suggest that sigma(54) is mainly involved in the control of carbohydrate metabolism in L. monocytogenes via direct regulation of PTS activity, alteration of the pyruvate pool and modulation of carbon catabolite regulation.
Arous
Safia
S
Equipe de Microbiologie Fondamentale et Appliquée, Université de Poitiers, 40 avenue du Recteur Pineau, 86022 Poitiers Cedex, France.
Buchrieser
Carmen
C
Laboratoire de Génomique des Micro-organismes Pathogènes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France.
Folio
Patrice
P
Station de Recherches sur la Viande, Institut National de la Recherche Agronomique de Theix, 63122 Saint-Genes Champanelle, France.
Glaser
Philippe
P
Laboratoire de Génomique des Micro-organismes Pathogènes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France.
Namane
Abdelkader
A
Plateforme de protéomique, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France.
Hébraud
Michel
M
Station de Recherches sur la Viande, Institut National de la Recherche Agronomique de Theix, 63122 Saint-Genes Champanelle, France.
Héchard
Yann
Y
Equipe de Microbiologie Fondamentale et Appliquée, Université de Poitiers, 40 avenue du Recteur Pineau, 86022 Poitiers Cedex, France.
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D050260:Carbohydrate Metabolism; D004268:DNA-Binding Proteins; D012321:DNA-Directed RNA Polymerases / Q000235:genetics* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D008089:Listeria monocytogenes / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011401:Promoter Regions, Genetic; D051877:RNA Polymerase Sigma 54; D012329:RNA, Bacterial / Q000378:metabolism; D012808:Sigma Factor / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012329:RNA, Bacterial; D012808:Sigma Factor; D012321:DNA-Directed RNA Polymerases; D051877:RNA Polymerase Sigma 54