ClC-3 expression in the cell cycle of nasopharyngeal carcinoma cells.
The immunofluorescence approach, the confocal microscopy and the patch-clamp technique were used to investigate the expression of ClC-3 (one of the candidates of volume-activated chloride channels) and its relationships with the volume-activated chloride current and the capacity of regulatory volume decrease (RVD) in the cell cycle of nasopharyngeal carcinoma cells (CNE-2Z cells). The results indicated that CNE-2Z cells expressed ClC-3. ClC-3 was located predominantly inside the cells but not in the membrane. Both the expression level and the distribution of ClC-3 were cell cycle dependent. ClC-3 expression was low in G1 but high in S phase. The cells in G2/M phase possessed an intermediate level of the expression. ClC-3 expression level was negatively correlated to the RVD capacity and amplitude of the volume-activated chloride current in the cell cycle. The results suggest that ClC-3 may be an important factor in the regulation of cell cycle progression, but that it is probably not the chloride channel associated with RVD in these cancer cells.
Wang
Li-Wei
LW
Department of Physiology and Laboratory of Cell Biology, Guangdong Medical College, Zhanjiang, Guangdong 524023, China. WANGLW@cardiff.ac.uk
Chen
Li-Xin
LX
Jacob
Tim
T
D000255:Adenosine Triphosphate / Q000494:pharmacology; D002453:Cell Cycle / Q000502:physiology*; D018118:Chloride Channels / Q000378:metabolism* / Q000502:physiology; D002712:Chlorides / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009124:Muscle Proteins / Q000378:metabolism / Q000502:physiology; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured
D018118:Chloride Channels; D002712:Chlorides; C115376:ClC-3 channel; D009124:Muscle Proteins; D000255:Adenosine Triphosphate