Characterization of proteases in the digestive system of spiny lobster (Panulirus interruptus).
Enzymes responsible for digestion of food protein were evaluated and characterized in red lobster (Panulirus interruptus). Several tissues, organs, and body fluids were analyzed. The same composition of proteases was found in gastric juice, midgut gland, and intestinal contents. Using specific substrates and inhibitors, we identified several isotrypsins and isochymotrypsins by gel electrophoresis. Protease activity was found at pH 3 and reduced by using pepstatin A. Operational variables of enzymes were characterized for management of future studies and potential biotechnologies. Types and activities of lobster digestive enzymes constitute background information to study the digestive abilities of the organism further, and will lead to understanding nutritional needs and feeding ecology, mainly because decapods display unique morphologic, metabolic, and behavioral changes during their life cycle. Also, such enzymes become alternative tools for use in biotechnologies.
Celis-Guerrero
Laura E
LE
Centro de Investigaciones Biológicas del Noroeste (CIBNOR), PO Box 129, La Paz, B.C.S. 23000, México.
García-Carreño
Fernando L
FL
del Toro
M Angeles Navarrete
MA
D000704:Analysis of Variance; D000818:Animals; D004064:Digestive System / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D033521:Palinuridae / Q000201:enzymology*; D010436:Pepstatins; D010447:Peptide Hydrolases / Q000302:isolation & purification*; D013696:Temperature; D013997:Time Factors
D010436:Pepstatins; D010447:Peptide Hydrolases; C031375:pepstatin