Exploring the context of the lung proteome within the airway mucosa following allergen challenge.
The lung proteome is a dynamic collection of specialized proteins related to pulmonary function. Many cells of different derivations, activation states, and levels of maturity contribute to the changing environment, which produces the lung proteome. Inflammatory cells reacting to environmental challenge, for example from allergens, produce and secrete proteins which have profound effects on both resident and nonresident cells located in airways, alveoli, and the vascular tree which provides blood cells to the parenchyma alveolar bed for gas exchange. In an experimental model of allergic airway inflammation, we have compared control and allergen challenged lung compartments to determine global protein expression patterns using 2D-gel electrophoresis and subsequent spot identification by MS/MS mass spectrometry. We have then specifically isolated the epithelial mucosal layer, which lines conducting airways, from control and allergen challenged lungs, using laser capture technology and performed proteome identification on these selected cell samples. A central component of our investigations has been to contextually relate the histological features of the dynamic pulmonary environment to the changes in protein expression observed following challenge. Our results provide new information of the complexity of the submucosa/epithelium interface and the mechanisms behind the transformation of airway epithelium from normal steady states to functionally activated states.
Fehniger
Thomas E
TE
AstraZeneca R&D, Respiratory and Inflammation, Department of Biological Sciences, S-221 87 Lund, Sweden.
Sato-Folatre
José-Gabriel
JG
Malmström
Johan
J
Berglund
Magnus
M
Lindberg
Claes
C
Brange
Charlotte
C
Lindberg
Henrik
H
Marko-Varga
György
G
D000485:Allergens / Q000737:chemistry*; D000818:Animals; D001249:Asthma / Q000473:pathology; D001980:Bronchi / Q000378:metabolism; D016535:Bronchial Hyperreactivity / Q000473:pathology; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D004804:Eosinophils / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D005260:Female; D007834:Lasers; D008168:Lung / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D013058:Mass Spectrometry; D051379:Mice; D008807:Mice, Inbred BALB C; D009092:Mucous Membrane / Q000473:pathology*; D011506:Proteins / Q000737:chemistry; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D012137:Respiratory System / Q000378:metabolism*
D000485:Allergens; D011506:Proteins; D020543:Proteome