[Isolation and purification of gonyautoxins from Alexandrium mimutum Halim].
OBJECTIVE
To isolate and purify gonyautoxins from Alexandrium mimutum Halim Amtk2 strain.


METHODS
The ethanol extracts of culture Alexandriun minutum Halim Amtk2 were isolated by means of gel filtration chromatography, the toxin fraction obtained was then purified by ion exchange chromatography.


RESULTS
From 100 liter of cultivation liquid of Alexandrium mimutum Halim Amtk2 (6.74 +/- 0.31) x 10(9) cells were obtained. The ethanol extracts of Alexandriun minutum Halim purified by gel filtration chromatography obtained gonyautoxins mixture 29.59 mg. 4.06 mg of the mixture was further purified by two steps of ion exchange chromatography, and obtained pure GTX-4 (0.40 +/- 0.002) mg, GTX-1 (5.95 +/- 0.03) x 10(-2) mg, GTX-3 (6.92 +/- 0.05) x 10(-4) mg and GTX-2 (0.11 +/- 0.005) mg.


CONCLUSIONS
Pure gonyautoxins can be obtained by means of gel filtration chromatography and ion exchange chromatography from ethanol extracts of cultured Alexandriun minutum Halim Amtk2 strain.
Miao
Yu-ping
YP
School of Pharmacy, Fu Dan University, Shanghai 200032, China.
Zhou
Hong-nong
HN
Wen
Ren
R
D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange; D004141:Dinoflagellida / Q000737:chemistry*; D008387:Marine Toxins / Q000737:chemistry / Q000302:isolation & purification; D015394:Molecular Structure; D012530:Saxitoxin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification*
D008387:Marine Toxins; D012530:Saxitoxin; C013007:gonyautoxins