title: Role of nucleostemin in growth regulation of gastric cancer, liver cancer and other malignancies.
abstract: OBJECTIVE
  To examine the role of nucleostemin in the growth regulation of gastric cancer, liver cancer and other cancers.
  
  
  METHODS
  RT-PCR was used to clone the fragment of nucleostemin cDNA from HEK 293 cells. Eighteen kinds of malignant tumor tissues including gastric adenocarcinoma and liver cancer tissues, 3 kinds of benign tumor tissues, 3 kinds of benign hyperplastic tissues and normal tissues were employed to examine nucleostemin gene expression by RT-PCR, Slot blot, Northern blot and in situ hybridization.
  
  
  RESULTS
  We successfully cloned a 570 bp fragment of nucleostemin-cDNA from HEK-293 cells. All detected malignant tumor tissues, benign tumor tissues, and benign hyperplastic tissues had high levels of nucleostemin expression. Nucleostemin was also expressed in human placenta tissue at a high level. In terminally differentiated normal human adult kidney and mammary gland tissues, no nucleostemin expression could be detected.
  
  
  CONCLUSIONS
  Nucleostemin can help regulate the proliferation of both cancer cells and stem cells. It might play an important role in the growth regulation of gastric cancer, liver cancer and other cancers.
authors.affiliation: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Si-Jin
authors.initials: SJ
authors.lastname: Liu
authors.forename: Zi-Wei
authors.initials: ZW
authors.lastname: Cai
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Liu
authors.forename: Mei-Yu
authors.initials: MY
authors.lastname: Dong
authors.forename: Li-Qiu
authors.initials: LQ
authors.lastname: Sun
authors.forename: Guo-Fa
authors.initials: GF
authors.lastname: Hu
authors.forename: Ying-Yun
authors.initials: YY
authors.lastname: Wei
authors.forename: Wei-De
authors.initials: WD
authors.lastname: Lao
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D005260:Female; D019204:GTP-Binding Proteins; D005786:Gene Expression Regulation; D006801:Humans; D017403:In Situ Hybridization; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D002352:Carrier Proteins; C469949:GNL3 protein, human; D009687:Nuclear Proteins; D019204:GTP-Binding Proteins