Analysis of the interaction of a hybrid system consisting of bovine adrenodoxin reductase and flavodoxin from the cyanobacterium Anabaena PCC 7119.
The mitochondrial steroid-hydroxylating system in vertebrates and the NADPH producing electron transfer chain in photosynthetic organisms contain structurally and functionally similar components. Examination of a potential hybrid reconstitution of the electron transfer chain between different components of both systems could help to improve our knowledge on protein-protein interaction and subsequent electron transfer. Here we analyzed the interaction between bovine adrenodoxin reductase and flavodoxin from the cyanobacterium Anabaena PCC 7119. Optical biosensor as well as steady state and fast kinetic experiments showed their ability to form distinct productive complexes. Compared with the corresponding physiological systems the electron transfer is rather slow, probably due to the lack of specificity at the interaction surface.
Zöllner
A
A
Fachrichtung 8.8-Biochemie, Universität des Saarlandes, P.O. Box 15 11 50, DE-66041, Saarbrücken, Germany.
Nogués
I
I
Heinz
A
A
Medina
M
M
Gómez-Moreno
C
C
Bernhardt
R
R
D000818:Animals; D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D003067:Coenzymes; D000458:Cyanobacteria / Q000201:enzymology*; D004579:Electron Transport; D004789:Enzyme Activation; D005287:Ferredoxin-NADP Reductase / Q000032:analysis* / Q000737:chemistry*; D005418:Flavodoxin / Q000032:analysis* / Q000737:chemistry*; D007700:Kinetics; D055095:Optics and Photonics; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*
D003067:Coenzymes; D005418:Flavodoxin; D005287:Ferredoxin-NADP Reductase