title: Precise excision of the large pathogenicity island, SPI7, in Salmonella enterica serovar Typhi.
abstract: The large pathogenicity island (SPI7) of Salmonella enterica serovar Typhi is a 133,477-bp segment of DNA flanked by two 52-bp direct repeats overlapping the pheU (phenylalanyl-tRNA) gene, contains 151 potential open reading frames, and includes the viaB operon involved in the synthesis of Vi antigen. Some clinical isolates of S. enterica serovar Typhi are missing the entire SPI7, due to its precise excision; these strains have lost the ability to produce Vi antigen, are resistant to phage Vi-II, and invade a human epithelial cell line more rapidly. Excision of SPI7 occurs spontaneously in a clinical isolate of S. enterica serovar Typhi when it is grown in the laboratory, leaves an intact copy of the pheU gene at its novel join point, and results in the same three phenotypic consequences. SPI7 is an unstable genetic element, probably an intermediate in the pathway of lateral transfer of such pathogenicity islands among enteric gram-negative bacteria.
authors.affiliation: Instituto de Ciencias Biomédicas, Universidad de Chile, Santiago, Chile.
authors.forename: Susan M
authors.initials: SM
authors.lastname: Bueno
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Santiviago
authors.forename: Alejandro A
authors.initials: AA
authors.lastname: Murillo
authors.forename: Juan A
authors.initials: JA
authors.lastname: Fuentes
authors.forename: A Nicole
authors.initials: AN
authors.lastname: Trombert
authors.forename: Paula I
authors.initials: PI
authors.lastname: Rodas
authors.forename: Philip
authors.initials: P
authors.lastname: Youderian
authors.forename: Guido C
authors.initials: GC
authors.lastname: Mora
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D006801:Humans; D008969:Molecular Sequence Data; D009876:Operon; D010641:Phenotype; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000235:genetics*; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics*; D011995:Recombination, Genetic; D012485:Salmonella typhi / Q000235:genetics* / Q000472:pathogenicity*
chemical_list: D000942:Antigens, Bacterial; D011135:Polysaccharides, Bacterial; D012346:RNA, Transfer, Amino Acyl