Role of endogenous lipids in the chromophore regeneration of bacteriorhodopsin.
The regeneration method of Khorana [J. Biol. Chem. 262 (1987) 9271] has been modified in order to study the effect of endogenous archaeabacterial lipids and, in particular, of glycocardiolipin (GlyC) in the refolding and chromophore regeneration of bacteriorhodopsin (BR). BR refolding and chromophore regeneration could be obtained in the presence of endogenous lipid mixtures containing or not containing glycocardiolipin; however, the kinetics of bacteriorhodopsin regeneration in the presence of glycocardiolipin was faster than in its absence. These results show for the first time that the interaction of glycocardiolipin with bacteriorhodopsin favours its refolding from the denaturated state and the chromophore regeneration.
Catucci
L
L
Dipartimento di Chimica, Università di Bari, Via Orabona 4, 70126 Bari, Italy.
Lattanzio
V M T
VM
Lobasso
S
S
Agostiano
A
A
Corcelli
A
A
D001436:Bacteriorhodopsins / Q000737:chemistry* / Q000528:radiation effects*; D002308:Cardiolipins / Q000737:chemistry* / Q000528:radiation effects*; D045424:Complex Mixtures / Q000737:chemistry / Q000528:radiation effects; D007700:Kinetics; D008027:Light; D008563:Membrane Lipids / Q000737:chemistry / Q000528:radiation effects; D011485:Protein Binding; D011489:Protein Denaturation / Q000528:radiation effects; D017510:Protein Folding
D002308:Cardiolipins; D045424:Complex Mixtures; D008563:Membrane Lipids; D001436:Bacteriorhodopsins