Effect of carbonic anhydrase-related protein VIII expression on lung adenocarcinoma cell growth.
Carbonic anhydrase-related protein VIII (CA-RP VIII) is expressed in most non-small cell lung cancer, and especially strongly at the front of tumor progression. Screening analysis of CA-RP VIII expression in a panel of cultured lung cancer cell lines showed that a well differentiated adenocarcinoma cell line, PC-9, appeared to lack CA-RP VIII. Subsequently, CA8 cDNA was transfected with an expression vector into PC-9. Ectopic overexpression of CA-RP VIII reduced the growth of PC-9 cells on uncoated culture dishes, especially when the cultures were started at low cell density, but increased cell growth on laminin-coated dishes. Interestingly, ectopic CA-RP VIII expression markedly reduced caspase-3 activity induced by serum starvation and anti-cancer agents in PC-9 cells. The present findings suggest that CA-RP VIII expression promotes progression of lung cancer by multifarious mechanisms.
Lu
Shu-hui
SH
Department of Tumor Pathology, Kochi Medical School, Nankoku, Kochi 783-8505, Japan.
Takeuchi
Tamotsu
T
Fujita
Jiro
J
Ishida
Toshihiko
T
Akisawa
Yousuke
Y
Nishimori
Isao
I
Kohsaki
Takuhiro
T
Onishi
Saburo
S
Sonobe
Hiroshi
H
Ohtsuki
Yuji
Y
D000230:Adenocarcinoma / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000494:pharmacology; D014408:Biomarkers, Tumor; D002256:Carbonic Anhydrases / Q000096:biosynthesis*; D018288:Carcinoma, Small Cell / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D020169:Caspases / Q000187:drug effects; D002455:Cell Division / Q000502:physiology; D045744:Cell Line, Tumor; D018450:Disease Progression; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D047908:Intracellular Signaling Peptides and Proteins
D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; D002256:Carbonic Anhydrases; D020169:Caspases; D009419:Nerve Tissue Proteins; C512821:Car8 protein, mouse; C512818:CA8 protein, human; D047908:Intracellular Signaling Peptides and Proteins