[Exoproteinases of the oomycete Phytophthora infestans].
When grown in a medium containing heat-stable potato tuber proteins, the oomycete Phytophthora infestans (Mont.) de Bary produces a set of exoproteinases active at neutral and mildly basic pH values. These extracellular proteinases have been shown by SDS-PAGE with the presence of gelatin to include at least six components differing in molecular weight. Inhibitory analysis and study of the effects of the enzymes on various synthetic substrates show that the culture liquid of P. infestans contains mainly serine proteinases specific to trypsin and subtilisin and metalloproteinases. Their activity is suppressed by proteinase-inhibitor proteins from potato tubers. It is suggested that P. infestans exoproteinases may be the metabolic target for natural proteinase inhibitors from potato.
Gvozdeva
E L
EL
Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 119071 Russia.
Ievleva
E V
EV
Gerasimova
N G
NG
Ozeretskovskaia
O L
OL
Valueva
T A
TA
D003470:Culture Media; D020689:Exopeptidases / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D045726:Metalloproteases / Q000032:analysis; D010838:Phytophthora / Q000201:enzymology* / Q000254:growth & development; D010940:Plant Proteins / Q000378:metabolism; D015842:Serine Proteinase Inhibitors / Q000378:metabolism; D011198:Solanum tuberosum / Q000378:metabolism; D013379:Substrate Specificity; D020860:Subtilisin / Q000032:analysis; D014357:Trypsin / Q000032:analysis
D003470:Culture Media; D010940:Plant Proteins; D015842:Serine Proteinase Inhibitors; D020689:Exopeptidases; D045726:Metalloproteases; D014357:Trypsin; D020860:Subtilisin