Functional expression of the ectoine hydroxylase gene (thpD) from Streptomyces chrysomallus in Halomonas elongata.
The formation of hydroxyectoine in the industrial ectoine producer Halomonas elongata was improved by the heterologous expression of the ectoine hydroxylase gene, thpD, from Streptomyces chrysomallus. The efficient conversion of ectoine to hydroxyectoine was achieved by the concerted regulation of thpD by the H. elongata ectA promoter.
Prabhu
Julia
J
ActinoDrug Pharmaceuticals GmbH, 16761 Hennigsdorf, Germany.
Schauwecker
Florian
F
Grammel
Nicolas
N
Keller
Ullrich
U
Bernhard
Michael
M
D000599:Amino Acids, Diamino / Q000096:biosynthesis / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D015964:Gene Expression Regulation, Bacterial; D020632:Halomonas / Q000201:enzymology* / Q000235:genetics; D006899:Mixed Function Oxygenases / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics
D000599:Amino Acids, Diamino; D001426:Bacterial Proteins; C121557:hydroxyectoine; C045628:ectoine; D006899:Mixed Function Oxygenases