Comparison of VITEK 2 with ITS2-fragment length polymorphism analysis for identification of yeast species.
A total of 61 clinical yeast isolates of Candida, Cryptococcus, Blastoschizomyces, and Saccharomyces spp. were used to compare two identification techniques, VITEK 2 and ITS2-fragment length polymorphism analysis (ITS2-FLP), with ID32C as the reference method. ID32C identified 58 isolates correctly. ITS2-FLP with Instagene DNA extraction identified 59 isolates. ITS2-FLP combined with boiling-freezing DNA extraction identified 55 isolates. VITEK 2 identified 41 isolates correctly.
Massonet
Caroline
C
Department of Immunology and Microbiology, Rega Institute, U.Z. Gasthuisberg, B-3000 Leuven, Belgium. caroline.massonet@med.kuleuven.ac.be
Van Eldere
Johan
J
Vaneechoutte
Mario
M
De Baere
Thierry
T
Verhaegen
Jan
J
Lagrou
Katrien
K
D002175:Candida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D003454:Cryptococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D009172:Mycology / Q000379:methods*; D009181:Mycoses / Q000175:diagnosis / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012440:Saccharomyces / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D004718:Saccharomycetales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D015003:Yeasts / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
D004271:DNA, Fungal