title: Antitrypanosomal activity of Brazilian propolis from Apis mellifera.
abstract: Extracts from different samples of Brazilian propolis were obtained by Soxhlet extraction or maceration at room temperature using ethanol, water, and accombination of both solvents. Analysis of their composition using HPLC revealed that no major differences were seen when a propolis sample was subject to different extraction methods. The activity of the 15 extracts was assayed against bloodstream trypomastigotes of Trypanosoma cruzi, the etiologic agent of Chagas' disease. Multivariate analysis was applied to evaluate the efficiency of the different extracts and the trypanocidal activity. The extracts could be divided into two groups. In the first, in which, extracts were obtained by reflux in Soxhlet using 100% ethanol, there was a lower content of bioactive compounds and consequently lower trypanocidal activity. Extract 136-Et100 stands out in this group, since it had the highest levels of bioactive compounds together with highest activity against the parasite when compared with all other extracts. The second group comprises extracts with intermediate levels of bioactive compounds and higher activity against T. cruzi.
authors.affiliation: Faculdade de Ciências Farmacêuticas, Universidade São Francisco, SP, Brazil.
authors.forename: Ildenize Barbosa
authors.initials: IB
authors.lastname: da Silva Cunha
authors.forename: Kelly
authors.initials: K
authors.lastname: Salomão
authors.forename: Mario
authors.initials: M
authors.lastname: Shimizu
authors.forename: Vassya S
authors.initials: VS
authors.lastname: Bankova
authors.forename: Angela Ramalho
authors.initials: AR
authors.lastname: Custódio
authors.forename: Solange Lisboa
authors.initials: SL
authors.lastname: de Castro
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Marcucci
mesh_terms: D000818:Animals; D001516:Bees; D001938:Brazil; D051379:Mice; D011429:Propolis / Q000302:isolation & purification / Q000494:pharmacology*; D014344:Trypanocidal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000502:physiology
chemical_list: D014344:Trypanocidal Agents; D011429:Propolis