Parasitic helminth fauna of the cutlass fish, Trichiurus lepturus L., and the differentiation of four anisakid nematode third-stage larvae by nuclear ribosomal DNA sequences.
The helminth fauna of the gastrointestinal tract and abdominal cavity of cutlass fish, Trichiurus lepturus L., off the Taiwanese coast of the north-western Pacific was investigated. The following helminths were found: (1) nematodes--Anisakis simplex, Hysterothylacium aduncum, Porrocaecum decipiens, Raphidascaris trichiuri; (2) digeneans--adult Lecithochirium trichiuri; and (3) cestodes-plerocercoids of Proteocephalus spp. The third-stage larvae of these four anisakid nematodes were characterized genetically using a molecular approach. The nuclear ribosomal DNA region spanning the first internal transcribed spacer (ITS-1), the 5.8S gene and the second internal transcribed spacer (ITS-2) was amplified and sequenced. Based on the sequence differences, a PCR-based restriction fragment length polymorphism method was established for the unequivocal delineation of the four species. Phylogenetic analysis showed that H. aduncum clustered with P. decipiens, whereas A. simplex was not closely related to these according to the nucleotide sequences of all rDNA.
Shih
Hsiu-Hui
HH
Department of Life Science and Institute of Zoology, National Taiwan University, 106 Taipei, Taiwan, Republic of China. shihhh@ntu.edu.tw
D034841:Abdominal Cavity / Q000469:parasitology; D000818:Animals; D017163:Anisakis / Q000145:classification / Q000302:isolation & purification; D001199:Ascaridoidea; D001483:Base Sequence; D002467:Cell Nucleus / Q000235:genetics; D002589:Cestoda / Q000145:classification* / Q000302:isolation & purification; D018509:DNA, Helminth / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000302:isolation & purification; D007422:Intestines / Q000469:parasitology; D007814:Larva / Q000033:anatomy & histology / Q000235:genetics / Q000648:ultrastructure; D008969:Molecular Sequence Data; D009348:Nematoda / Q000145:classification* / Q000302:isolation & purification; D010140:Pacific Ocean; D010473:Perciformes / Q000469:parasitology*; D010802:Phylogeny; D020161:Physical Chromosome Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013624:Taiwan; D014200:Trematoda / Q000145:classification* / Q000302:isolation & purification
D018509:DNA, Helminth; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer