title: Microcapsule modification with peroxidase-catalyzed phenol polymerization.
abstract: A biocatalytic polymer synthesis on a surface of polyelectrolyte microcapsules was studied. Horseradish peroxidase assembled in nanoorganized capsule walls by alternate adsorption with linear polyions retains its activity in reactions of enzyme-catalyzed polymerization of 4-oxyphenols. It allowed controllable synthesis of a phenolic polymer layer on microcapsule walls using an outermost surface peroxidase layer as a template. By varying the phenol type, buffer pH, and reaction component concentrations, the phenolic polymer coating of the capsules with a thickness in the range 20-50 nm was formed. The polymeric products are fluorescent, which provided a good opportunity for confocal image analysis of the capsule wall structure and the attached layer. The influence of a phenolic polymer layer on the permeability of the capsule walls was investigated.
authors.affiliation: Institute for Micromanufacturing and Chemistry Program, Louisiana Tech University, Ruston, Louisiana 71272, USA.
authors.forename: Tatsiana
authors.initials: T
authors.lastname: Shutava
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Zheng
authors.forename: Vijay
authors.initials: V
authors.lastname: John
authors.forename: Yuri
authors.initials: Y
authors.lastname: Lvov
mesh_terms: D002384:Catalysis; D004573:Electrolytes; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D010636:Phenols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004573:Electrolytes; D010636:Phenols; D011108:Polymers