Encapsulation of crosslinked penicillin G acylase aggregates in lentikats: evaluation of a novel biocatalyst in organic media.
The encapsulation of crosslinked enzyme aggregates (CLEA) of penicillin G acylase into a very rigid polymeric matrix based on polyvinyl alcohol (LentiKats) has been used successfully to improve the inadequate mechanical properties of CLEA. This encapsulation decreased CLEA activity by only around 40%. As compensation, a significant improvement in the stability of the CLEA in the presence of organic solvents was detected. This could be related to the highly hydrophilic environment inside the LentiKats biocatalysts: Partition experiments showed that the concentration of dioxane inside LentiKats was lower than in the reaction medium. In fact, thermal stability was about the same as in the corresponding CLEA. This permitted great improvement in the reaction rate for thermodynamically controlled synthesis of a model antibiotic (using phenylacetic acid and 7-amino-deacetoxycefalosporanic acid). Even more importantly, yields could be improved by using LentiKats-encapsulated CLEA, very likely by a favorable product/substrate partition. Thus, this very simple technique not only provides an efficient technique for solving the mechanical stability problem associated with CLEA, but also greatly improves the behavior of CLEA in organic media.
Wilson
Lorena
L
Departamento de Biocatalisis, Instituto de Catalisis,Campus UAM Cantoblanco, 28049 Madrid, Spain.
Illanes
Andrés
A
Pessela
Benevides C C
BC
Abian
Olga
O
Fernández-Lafuente
Roberto
R
Guisán
José M
JM
D000042:Absorption; D002384:Catalysis; D002511:Cephalosporins / Q000138:chemical synthesis*; D003432:Cross-Linking Reagents / Q000737:chemistry; D019281:Dimerization; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry; D006868:Hydrolysis; D009930:Organic Chemicals / Q000737:chemistry; D010399:Penicillin Amidase / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D013696:Temperature
D002511:Cephalosporins; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D020100:Hydrogels; D009930:Organic Chemicals; C025578:deacetoxycephalosporin C; D011142:Polyvinyl Alcohol; D010399:Penicillin Amidase