title: Automated sample preparation for the TRUGENE HIV-1 genotyping kit using the MagNA pure LC instrument.
abstract: Use of the MagNA Pure LC Total Nucleic Acid Isolation Kit-Large Volume in conjunction with the TRUGENE HIV-1 Genotyping Kit yielded consistent, interpretable sequence data from 1-mL plasma preparations containing HIV-1 RNA concentrations of > or =400 copies/mL. This finding was confirmed in 18 of 24 low-titer clinical specimens.
authors.affiliation: Division of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Germer
authors.forename: Jill N
authors.initials: JN
authors.lastname: Vandenameele
authors.forename: P Shawn
authors.initials: PS
authors.lastname: Mitchell
authors.forename: W Scott
authors.initials: WS
authors.lastname: Harmsen
authors.forename: Joseph D C
authors.initials: JD
authors.lastname: Yao
mesh_terms: D001331:Automation; D005838:Genotype; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D012367:RNA, Viral / Q000032:analysis; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012494:Sampling Studies; D012680:Sensitivity and Specificity
chemical_list: D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic