title: Immunoadjuvant potential of Asparagus racemosus aqueous extract in experimental system.
abstract: The immunoadjuvant potential of Asparagus racemosus (Willd.) Family (Liliaceae) aqueous root extract was evaluated in experimental animals immunized with diphtheria, tetanus, pertussis (DTP) vaccine. Immunostimulation was evaluated using serological and hematological parameters. Oral administration of test material at 100 mg/kg per day dose for 15 days resulted significant increase (P = 0.0052) in antibody titers to Bordtella pertussis as compared to untreated (control) animals. Immunized animals (treated and untreated) were challenged with B. pertussis 18323 strain and the animals were observed for 14 days. Results indicate that the treated animals did show significant increase in antibody titers as compared to untreated animals after challenge (P = 0.002). Immunoprotection against intra-cerebral challenge of live B. pertussis cells was evaluated based on degree of sickness, paralysis and subsequent death. Reduced mortality accompanied with overall improved health status was observed in treated animals after intra-cerebral challenge of B. pertussis indicating development of protective immune response. Present study indicates applications of test material as potential immunoadjuvant that also offers direct therapeutic benefits resulting in less morbidity and mortality.
authors.affiliation: Interdisciplinary School of Health Sciences, University of Pune, Pune, Maharastra 411007, India. gautam_monty@hotmail.com
authors.forename: Manish
authors.initials: M
authors.lastname: Gautam
authors.forename: Sham
authors.initials: S
authors.lastname: Diwanay
authors.forename: Sunil
authors.initials: S
authors.lastname: Gairola
authors.forename: Yojana
authors.initials: Y
authors.lastname: Shinde
authors.forename: Pralhad
authors.initials: P
authors.lastname: Patki
authors.forename: Bhushan
authors.initials: B
authors.lastname: Patwardhan
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000187:drug effects* / Q000276:immunology; D027761:Asparagus Plant; D001886:Bordetella pertussis / Q000276:immunology*; D015721:Diphtheria-Tetanus-Pertussis Vaccine / Q000276:immunology*; D005260:Female; D008297:Male; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots; D011897:Random Allocation
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D010936:Plant Extracts