[Primary study on measuring the internal transcribed spacer I regions of rRNA genein seeds of Gentiana dahurica].
OBJECTIVE
To amplify the PCR with the internal transcribed spacerl regions measure the base sequence of the amplified products of DNA, and to set up an identified standard on the level of molecule.


METHODS
DNA from the seeds of G. dahurica was extracted by conventional method, and composed peculiar primer was used to amplify with the internal transcribed spacerl regions of the rRNA gene, and the base sequence of the amplified products by stopping the circle of the end of double deoxidation of four color fluorescent mark was measured.


RESULTS
It was proved by agar sugar gel electrophoresis that the PCR amplified products of the internal transcribed spacerl regions of the rRNA gene existed. The base sequence of the seeds of G. dahurica's internal transcribed spacerl regions of the rRNA gene was measured.


CONCLUSIONS
To measure the base sequence of internal transcribed spacerl regions of the rRNA gene in the seeds of G. dahurica's is a method to identify vegetal Chinese traditional medicine on the level of molecule.
Ji
Ke-ping
KP
Gansu College of Traditional Chinese Medicine, Gansu 730000, Lanzhou, China.
Zhang
Xi-ling
XL
Liu
Li-sha
LS
Lu
Quan-yun
QY
Cheng
Che
C
D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D030012:Gentiana / Q000235:genetics*; D008969:Molecular Sequence Data; D010946:Plants, Medicinal / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics*; D012639:Seeds / Q000235:genetics
D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal