title: Spectrofluorometric determination of hydrogen peroxide based on oxidative catalytic reactions of p-hydroxyphenyl derivatives with metal complexes of thiacalix[4]arenetetrasulfonate on a modified anion-exchanger.
abstract: The peroxidase-like catalytic activity of metal complexes of thiacalix[4]arenetetrasulfonate (TCAS[4]) on a modified anion-exchanger (Me(n+)-TCAS[4]A-500; Me(n+) = H2, Fe3+, Fe2+, Mn3+, Co3+, Co2+, Cu2+, Zn2+, Ni2+) for the oxidation of p-hydroxyphenyl derivatives to produce fluorescent substances in the presence of hydrogen peroxide has been investigated. Among the Me(n+)-TCAS[4]A-500 tested, Fe(3+)-TCAS[4]A-500 exhibited the highest level of catalytic activity for the oxidation of p-acetoamidophenol in a carbonate buffer solution of pH 10. The catalytic activity of Fe(3+)-TCAS[4]A-500 was then used for the spectrofluorometric determination of hydrogen peroxide. The calibration curve for the Fe(3+)-TCAS[4]A-500 method was linear over a range spanning from 0.1 to 5.0 microg of hydrogen peroxide in a 1.0 ml sample solution.
authors.affiliation: Department of Biological Chemistry, Faculty of Science, Okayama University of Science, Ridai-cho, Okayama 700-0005, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Odo
authors.forename: Kozue
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Eri
authors.initials: E
authors.lastname: Shinmoto
authors.forename: Yoko
authors.initials: Y
authors.lastname: Hatae
authors.forename: Atsushi
authors.initials: A
authors.lastname: Shiozaki