title: [Studies on callus growth and phillyrin accumulation of Forsythia suspensa].
abstract: OBJECTIVE
  To investigate the effects of physical and chemical factors on callus growth and phillyrin contents of F. suspensa.
  
  
  METHODS
  The cell growth index and phyllirin yield in different culture condition such as different plant hormones mixed, mediums, light and dark were compared. HPLC was used to examine phillyrin contents.
  
  
  CONCLUSIONS
  Growth cycle of cells is twenty-eight days. During the course of callus growth, the processes of phillyrin biosynthesis were parallel with the cell growth. The optimum medium is MS. The optimum hormones concentrations are 1 mg.L-1 2,4-D, 0.5 mg.L-1 6-BA and 0.5 mg.L-1KT. The cell culture in light is more suitable than that in dark.
authors.affiliation: School of Life Science, Hubei University, Wuhan 430062, Hubei, China.
authors.forename: You-qi
authors.initials: YQ
authors.lastname: Liu
authors.forename: Xing-chun
authors.initials: XC
authors.lastname: Tang
authors.forename: De-tian
authors.initials: DT
authors.lastname: Cai
mesh_terms: D003470:Culture Media; D046508:Culture Techniques; D031657:Forsythia / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D005960:Glucosides / Q000096:biosynthesis*; D008029:Lighting; D010937:Plant Growth Regulators / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism
chemical_list: D003470:Culture Media; D005960:Glucosides; D010937:Plant Growth Regulators; C075528:phillyrin