[Sbudies on the effect of parthenolide on the proliferation of rat vascular smooth muscle cells and the mechanism].
OBJECTIVE
To study the effect of parthenolide on the proliferation of vascular smooth muscle cell(VSMC) and its mechanism.


METHODS
Vascular smooth muscle cell was cultured, the protein levels of c-fos, c-myc, p15, p16, p18, p19 were measured by Western blot method, cell cycle were examined with flow cytometry, and the DNA synthesis was determined by [3H]-TdR incorporation.


RESULTS
Parthenolide inhibited protein levels of c-fos, c-myc in a time-dependent manner but didn't affect the protein levels of p15, p16, p18, p19. Flow cytometric DNA analysis revealed that parthenolide increased significantly G0/G1 phase of VSMC and decreased S phase of VSMC in a dose-dependent manner. Parthenolide inhibited [3H]-TdR incorporation in a dose dependent manner.


CONCLUSIONS
Parthenolide may inhibit proliferation of VSMC by inhibiting the expressions of c-fos, c-myc, but not the expressions of p15, p16, p18, p19.
Weng
Shao-xiang
SX
Department of Cardiology, Second Affiliated Hospital, Medical School, Zhejiang University, Hangzhou 310009, Zhejiang, China. wengshaox@sina.com
Shan
Jiang
J
Lin
Xiao-xia
XX
Fu
Guo-sheng
GS
D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology; D019659:Asteraceae / Q000737:chemistry; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000494:pharmacology*
D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc; D012717:Sesquiterpenes; C002669:parthenolide