title: Increased adrenomedullin expression in lungs in endotoxaemia.
abstract: Adrenomedullin (AM) is a peptide involved in cardiovascular homeostasis and in inflammation. We examined its expression in a rat model of endotoxaemia. Male Sprague-Dawley rats received intraperitoneal injection of 5 or 10 mg/kg lipopolysaccharide (LPS), or saline as control. Rats were killed at 1, 3, 6, 12 and 24 h after injection. LPS at 5 mg/kg, but not saline, increased plasma AM significantly at 3 h. At 10 mg/kg, plasma AM was raised at 3, 6 and 12 h. Immunoreactive AM concentration in lung increased after 5 or 10 mg/kg LPS, but not saline. PreproAM mRNA level in lung was significantly increased at 3 and 6 h. In conclusion, endotoxin stimulates the expression of AM in the lungs and increases its circulatory concentration. AM may be involved in the systemic response to sepsis.
authors.affiliation: Department of Medicine, University of Hong Kong, Hong Kong, China. mycheung@hkucc.hku.hk
authors.forename: B M Y
authors.initials: BM
authors.lastname: Cheung
authors.forename: I S S
authors.initials: IS
authors.lastname: Hwang
authors.forename: C Y Y
authors.initials: CY
authors.lastname: Li
authors.forename: W-S
authors.initials: WS
authors.lastname: O
authors.forename: K W T
authors.initials: KW
authors.lastname: Tsang
authors.forename: R Y H
authors.initials: RY
authors.lastname: Leung
authors.forename: C R
authors.initials: CR
authors.lastname: Kumana
authors.forename: F
authors.initials: F
authors.lastname: Tang
mesh_terms: D053607:Adrenomedullin; D000818:Animals; D019446:Endotoxemia / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D008070:Lipopolysaccharides; D008168:Lung / Q000737:chemistry*; D008297:Male; D023421:Models, Animal; D010455:Peptides / Q000032:analysis* / Q000097:blood; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D008070:Lipopolysaccharides; D010455:Peptides; D011498:Protein Precursors; D012333:RNA, Messenger; C112034:preproadrenomedullin; D053607:Adrenomedullin