title: [Activation of Ca(2+)-activated K+ channels by oxyphenamone in rabbit mesenteric vascular smooth muscle cells].
abstract: OBJECTIVE
  To study the effects of oxyphenamone (Oxy) on activation of Ca(2+)-activated K+ channels in rabbit mesenteric vascular smooth muscle cells.
  
  
  METHODS
  To measure the effect of Oxy on the Ca(2+)-activated K+ channel (BK (Ca) channel) activity in rabbit mesenteric vascular smooth muscle cells by using whole cell patch clamp techniques.
  
  
  RESULTS
  Oxy reversibly increase BK (Ca) channel activity in rabbit mesenteric artery smooth muscle cells. Application of Oxy (0.1 mumol.L-1) to the perfusion solution caused significant increase in outward currents and its effect was completely abolished by washout; The outward currents K+ was inhibited by TEA (7.5 mmol.L-1); Oxy activated the BK (Ca) channel in a dose-dependent manner (0.01-10 mumol.L-1).
  
  
  CONCLUSIONS
  Oxy directly increase the activity of BK (Ca) channel activity in rabbit mesenteric vascular smooth muscle cells in dose-dependent manner.
authors.affiliation: Department of Pharmacology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China.
authors.forename: An-long
authors.initials: AL
authors.lastname: Li
authors.forename: Zhong-wu
authors.initials: ZW
authors.lastname: Liu
authors.forename: Li-xia
authors.initials: LX
authors.lastname: Zhu
authors.forename: De-chang
authors.initials: DC
authors.lastname: Zhang
authors.forename: Yi-xin
authors.initials: YX
authors.lastname: Ye
mesh_terms: D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology*; D008638:Mesenteric Arteries / Q000166:cytology; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000502:physiology*; D009930:Organic Chemicals; D018408:Patch-Clamp Techniques; D024681:Potassium Channels, Calcium-Activated / Q000187:drug effects*; D011817:Rabbits
chemical_list: D002316:Cardiotonic Agents; D009930:Organic Chemicals; D024681:Potassium Channels, Calcium-Activated; C442156:oxyphenamone