title: Microdialysis combined with liquid chromatography-tandem mass spectrometry for the determination of 6-aminobutylphthalide and its main metabolite in the brains of awake freely-moving rats.
abstract: 6-Aminobutylphthalide (ABP) is a new drug candidate which is currently being developed for the treatment of cerebral ischemia. The pharmacokinetics and metabolism of ABP were studied using in situ microdialysis sampling in the brains of awake freely-moving rats. Two LC-MS/MS methods were used for the quantitative and qualitative analysis of microdialysate. For comparison and confirmation, brain tissue samples were also analyzed by LC-MS/MS and GC/MS. The results described provide more authentic information in pharmacokinetics and metabolism at the site of action by using the coupling of microdialysis to LC-MS/MS technique than the traditional sampling methods.
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, 1 Xian Nong Tan Street, Beijing 100050, China.
authors.forename: Jin-ping
authors.initials: JP
authors.lastname: Qiao
authors.forename: Zeper
authors.initials: Z
authors.lastname: Abliz
authors.forename: Feng-ming
authors.initials: FM
authors.lastname: Chu
authors.forename: Pei-ling
authors.initials: PL
authors.lastname: Hou
authors.forename: Li-yan
authors.initials: LY
authors.lastname: Zhao
authors.forename: Min
authors.initials: M
authors.lastname: Xia
authors.forename: Yan
authors.initials: Y
authors.lastname: Chang
authors.forename: Zong-ru
authors.initials: ZR
authors.lastname: Guo
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D008297:Male; D017551:Microdialysis; D051381:Rats; D017208:Rats, Wistar
chemical_list: C493999:6-Aminobutylphthalide; D001572:Benzofurans