Influence of IL-1 beta and TNF-alpha on Fas expression of human retinal pigment epithelial cells in vitro.
OBJECTIVE
To observe Fas expression change of cultured human retinal pigment epithelial (RPE) cells by IL-1 beta and TNF-alpha.


METHODS
With flow cytometry, immunohischemistry, and color imaging system, Fas expressions by exposure to IL-1 beta and/or TNF-alpha were measured.


RESULTS
The gray degree values of Fas expression were 67.5 +/- 6.1 in IL-1 beta + TNF-alpha-treated group, 80.1 +/- 9.2 in IL-1 beta-treated group, and 70.4 +/- 6.4 in TNF-alpha-treated group, respectively. There were significant differences (P < 0.005) compared with control group (107.0 +/- 10.2). Flow cytometry showed that 15.0% cultured human RPE cells expressed Fas. Fas-positive in IL-1 beta, TNF-alpha, and IL-1 beta + TNF-alpha-treated groups expressed was 28.1%, 34.5%, and 65.2%, respectively.


CONCLUSIONS
IL-1 beta, TNF-alpha, and combining both of them can up-regulate Fas protein expression, which may contribute to more Fas (+) cells in proliferative vitreoretinopathy (PVR). Minimizing this process by means of inducing apoptosis of Fas (+) proliferative cells of Fas/FasL pathway is a future preventive and therapeutic possibility for PVR.
Liu
Bing
B
Department of Ophthalmology, Air Force General Hospital, Beijing 100036, China.
Ma
Jixian
J
Wei
Hong
H
Hui
Yannian
Y
D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D053222:Fas Ligand Protein; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D015854:Up-Regulation; D019014:fas Receptor / Q000096:biosynthesis* / Q000235:genetics
C505661:FASLG protein, human; D053222:Fas Ligand Protein; D007375:Interleukin-1; D008562:Membrane Glycoproteins; D014409:Tumor Necrosis Factor-alpha; D019014:fas Receptor