Effects of copper-aspirin complex on platelet-neutrophil interactions.
OBJECTIVE
To investigate the effects of copper-aspirin complex on rat thrombosis and the interaction between platelets and neutrophils.


METHODS
The model of electrically stimulated carotid artery thrombosis in Sprague Dawley rats was used; the effects of copper-aspirin complex on rat platelet-neutrophil adhesion and platelet aggregation stimulated by activated neutrophils were observed by rosette assay and Born's method, respectively.


RESULTS
Intragastric copper-aspirin complex (5, 7, and 10 mg/kg) dose-dependently prolonged the occlusion time; it significantly decreased the rosette number formed between thrombin-activated platelets and neutrophils; the 50 % of inhibitory concentration (IC50) was (54.6+/-4.3) micromol/L. Copper-aspirin complex markedly inhibited rat platelet aggregation induced by either cell free supernatant of activated neutrophils or by activated neutrophil suspension. The values of IC50 were (224.5+/-16.2) micromol/L and (820.5+/-21.4) micromol/L, whereas aspirin had no influence.


CONCLUSIONS
Copper-aspirin complex inhibited platelet-neutrophil interactions through a different property from aspirin and resulted in a more potent antithrombotic activity.
Shen
Zhi-qiang
ZQ
Yunnan Pharmacological Laboratories of Natural Products, Kunming Medical College, Kunming 650031, China. szq21cn@hotmail.com
Chen
Peng
P
Li
Ling
L
Chen
Peng
P
Liu
Wei-ping
WP
D000818:Animals; D001241:Aspirin / Q000494:pharmacology*; D001792:Blood Platelets / Q000502:physiology*; D002341:Carotid Artery Thrombosis / Q000097:blood*; D002448:Cell Adhesion / Q000187:drug effects; D003300:Copper / Q000494:pharmacology*; D004338:Drug Combinations; D008297:Male; D009504:Neutrophils / Q000502:physiology*; D010973:Platelet Adhesiveness / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley
D004338:Drug Combinations; D003300:Copper; D001241:Aspirin