title: Association of maternal histocompatibility at class II HLA loci with maternal microchimerism in the fetus.
abstract: For investigating the possible influence of maternal-fetal HLA compatibility on maternal microchimerism, DNA samples from blood of 120 maternal-fetal pairs were genotyped at two polymorphic loci: glutathione-S-transferase M1 (GSTM1) and angiotensin-converting enzyme (ACE). Informative pairs (mother heterozygous/fetus homozygous at one of the two loci) were then tested by quantitative real-time PCR for the noninherited maternal allele(s) and genotyped at the HLA-A, B, and C class I loci and/or at the DRB1 and/or DQB1 class II loci. Small numbers of maternal cells were detected in the circulation of 16 of the 30 informative second- and third-trimester fetuses. Comparison with HLA data suggested an association between microchimerism and maternal compatibility at the class II DRB1 and/or DQB1 HLA loci and with the maternal HLA-DQB1*0301 allele. There was no relationship between maternal microchimerism and maternal-fetal HLA compatibility at other HLA loci or with gestational age, fetal anomalies, or red cell or platelet isoimmunity.
authors.affiliation: Division of Maternal-Fetal Medicine, Wayne State University, Detroit, MI 48201, USA.
authors.forename: Stanley M
authors.initials: SM
authors.lastname: Berry
authors.forename: Sonia S
authors.initials: SS
authors.lastname: Hassan
authors.forename: Evelyne
authors.initials: E
authors.lastname: Russell
authors.forename: Debra
authors.initials: D
authors.lastname: Kukuruga
authors.forename: Susan
authors.initials: S
authors.lastname: Land
authors.forename: Joseph
authors.initials: J
authors.lastname: Kaplan
mesh_terms: D000328:Adult; D046528:Chimerism / Q000706:statistics & numerical data*; D005260:Female; D005312:Fetal Blood / Q000502:physiology; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D008431:Maternal-Fetal Exchange / Q000235:genetics*; D011247:Pregnancy; D015995:Prevalence
chemical_list: D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains