title: MAGP-2 has multiple binding regions on fibrillins and has covalent periodic association with fibrillin-containing microfibrils.
abstract: The interactions of microfibril-associated glycoprotein (MAGP)-2 have been investigated with fibrillins and fibrillin-containing microfibrils. Solid phase binding assays were conducted with recombinant fragments covering fibrillin-1 and most of fibrillin-2. MAGP-2, and its structure relative MAGP-1, were found to bind two fragments spanning the N-terminal half of fibrillin-1 and an N-terminal fragment of fibrillin-2. Blocking experiments indicated that MAGP-2 had a binding site(s) close to the N terminus of the fibrillin-1 molecule that was distinct from that for MAGP-1 and an additional, more central binding site(s) that may be shared by the two MAGPs. Immunogold labeling of developing nuchal ligament tissue showed that MAGP-2 had regular covalent and periodic (about 56 nm) association with fibrillin-containing microfibrils of elastic fibers in this tissue. Further analysis of isolated microfibrils indicated that MAGP-2 was attached at two points along the microfibril substructure, "site 1" on the "beads" and "site 2" at the "shoulder" of the interbead region close to where the two "arms" fuse. In contrast, MAGP-1 was located only on the beads. Comparison of the MAGP-2 binding data with known fibrillin epitope maps of the microfibrils showed that site 1 correlated with the N-terminal MAGP-2 binding region, and site 2 correlated with the second, more central, MAGP-2 binding region on the fibrillin-1 molecule. Of particular note, immunolabeling at site 2 was markedly decreased, relative to that at site 1, on extended microfibrils with bead-to-bead periods over 90 nm, suggesting that site 2 may move toward the beads when the microfibril is stretched. The study points to MAGP-2 being an integral component of some populations of fibrillin-containing microfibrils. Moreover, the identification of multiple MAGP-binding sequences on fibrillins supports the concept that MAGPs may function as molecular cross-linkers, stabilizing fibrillin monomers in folded conformation within or between the microfibrils, and thus MAGPs may be implicated in the modulation of the elasticity of these structures.
authors.affiliation: Department of Pathology, University of Adelaide, Adelaide, SA 5005, South Australia, Australia.
authors.forename: Eric
authors.initials: E
authors.lastname: Hanssen
authors.forename: Fan Hing
authors.initials: FH
authors.lastname: Hew
authors.forename: Emma
authors.initials: E
authors.lastname: Moore
authors.forename: Mark A
authors.initials: MA
authors.lastname: Gibson
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002460:Cell Line; D003285:Contractile Proteins / Q000378:metabolism*; D004547:Elastic Tissue / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D000939:Epitopes; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D000071838:Fibrillin-1; D000071840:Fibrillin-2; D000071837:Fibrillins; D006801:Humans; D007150:Immunohistochemistry; D008022:Ligaments / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D020894:Microfibrils / Q000378:metabolism*; D008840:Microfilament Proteins / Q000737:chemistry / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D000072260:RNA Splicing Factors; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D054392:Adipokines
chemical_list: D003285:Contractile Proteins; D000939:Epitopes; D016326:Extracellular Matrix Proteins; D000071838:Fibrillin-1; D000071840:Fibrillin-2; D000071837:Fibrillins; D008840:Microfilament Proteins; D010446:Peptide Fragments; D000072260:RNA Splicing Factors; D011994:Recombinant Proteins; C000605722:FBN1 protein, human; D054392:Adipokines; C000605723:FBN2 protein, human; C016598:microfibrillar protein