title: Treatment of full-thickness skin defect with concomitant grafting of 6-fold extended mesh auto-skin and allogeneic cultured dermal substitute.
abstract: The aim of this clinical study was to evaluate an allogeneic cultured dermal substitute (CDS) as a biological dressing for highly extended mesh auto-skin grafting. The subjects were five patients with extensive deep burn wounds. Allogeneic CDS was prepared by seeding fibroblasts on a spongy matrix of hyaluronic acid and atelo-collagen. Six-fold extended auto-skin graft was applied to the debrided wound, on which allogeneic CDS was placed. A conventional ointment-gauze dressing was used to protect the CDS. The CDS was applied repeatedly at intervals of 5-7 days. In all cases, the wounds were closed by successful take of mesh auto-skin graft and prompt epithelization from the grafted skin. The skin on the grafted area had a cicatrix appearance, but was soft and thin, maintaining good quality. The application of 6-fold extended auto-skin graft in conjunction with allogeneic CDS is an effective method for treatment of extensive severe burn wounds.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Kagawa Prefectural Central Hospital, Takamatsu, Kagawa, Japan.
authors.forename: Naohiro
authors.initials: N
authors.lastname: Kashiwa
authors.forename: Osamu
authors.initials: O
authors.lastname: Ito
authors.forename: Takeshige
authors.initials: T
authors.lastname: Ueda
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kubo
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Matsui
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Kuroyanagi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002056:Burns / Q000601:surgery; D062109:Dermatologic Surgical Procedures; D020405:Dermis / Q000254:growth & development; D005260:Female; D005347:Fibroblasts; D006801:Humans; D008297:Male; D012867:Skin / Q000378:metabolism; D016038:Skin Transplantation / Q000379:methods*; D017768:Skin, Artificial; D013526:Surgical Mesh; D023822:Tissue Engineering; D014184:Transplantation, Homologous; D016896:Treatment Outcome