Similar genotypes of Pneumocystis jirovecii in different forms of Pneumocystis infection.
This study describes the genotyping of Pneumocystis jirovecii organisms isolated from three groups of patients that developed diverse forms of P. jirovecii infection; the patients were monitored in the same French hospital. Forty archival specimens from 13 adults with Pneumocystis pneumonia, eight adults colonized by P. jirovecii and 19 immunocompetent infants infected with the fungus contemporaneously with a bronchiolitis episode were analysed retrospectively. Genotyping was performed by analysis of sequences of the internal transcribed spacer (ITS)1 and ITS2 regions, and of the dihydropteroate synthase (DHPS) locus. At the ITS regions, a high diversity of genotypes, identical main genotypes (B(1)a(3) and B(2)a(1)) and the occurrence of mixed infections (more than one genotype) were observed in the three patient groups. At the DHPS locus, the results indicated the presence of mutants in the two adult groups, as well as in the infant group. Consequently, at these two independent genomic regions, P. jirovecii isolates from patients who developed different forms of infection and who lived in the same geographical region presented common characteristics. These results suggest that patients infected with P. jirovecii, whatever the form of infection they present, are part of a common human reservoir for P. jirovecii.
Totet
Anne
A
Service de Parasitologie, Mycologie et Médecine des Voyages, Hôpital Sud, Centre Hospitalier Universitaire, Université de Picardie Jules Verne, 1 rue Laennec, 80054 Amiens, Paris, France.
Duwat
Hyacinthe
H
Service de Parasitologie, Mycologie et Médecine des Voyages, Hôpital Sud, Centre Hospitalier Universitaire, Université de Picardie Jules Verne, 1 rue Laennec, 80054 Amiens, Paris, France.
Magois
Eline
E
Service de Parasitologie, Mycologie et Médecine des Voyages, Hôpital Sud, Centre Hospitalier Universitaire, Université de Picardie Jules Verne, 1 rue Laennec, 80054 Amiens, Paris, France.
Jounieaux
Vincent
V
Service de Parasitologie, Mycologie et Médecine des Voyages, Hôpital Sud, Centre Hospitalier Universitaire, Université de Picardie Jules Verne, 1 rue Laennec, 80054 Amiens, Paris, France.
Roux
Patricia
P
Centre Hospitalier Universitaire Saint Antoine, Paris, France.
Raccurt
Christian
C
Service de Parasitologie, Mycologie et Médecine des Voyages, Hôpital Sud, Centre Hospitalier Universitaire, Université de Picardie Jules Verne, 1 rue Laennec, 80054 Amiens, Paris, France.
Nevez
Gilles
G
Service de Parasitologie, Mycologie et Médecine des Voyages, Hôpital Sud, Centre Hospitalier Universitaire, Université de Picardie Jules Verne, 1 rue Laennec, 80054 Amiens, Paris, France.
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001988:Bronchiolitis / Q000382:microbiology*; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology*; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D004094:Dihydropteroate Synthase / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016720:Pneumocystis Infections / Q000382:microbiology*; D045363:Pneumocystis carinii / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D011020:Pneumonia, Pneumocystis / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D004094:Dihydropteroate Synthase