title: Comparison of adolescent and young adult self-collected and clinician-collected samples for human papillomavirus.
abstract: OBJECTIVE
  To examine the concordance between self-collected and clinician-collected samples for human papillomavirus (HPV) DNA.
  
  
  METHODS
  Sexually active adolescent and young adult women aged 14-21 years (N = 101) were enrolled in a prospective cohort study of HPV testing. Participants self-collected vaginal samples for HPV DNA, and clinicians collected cervicovaginal samples for HPV DNA and a cervical cytology specimen. We determined concordance between the results of self- and clinician-collected specimens using a kappa statistic and McNemar's test.
  
  
  RESULTS
  Of the 51% of participants who were HPV positive, 53% had 1 type, 25% had 2 types, and 22% had 3 types or more; 25 different HPV types were identified. Self-collected samples detected more participants with HPV than clinician-collected samples (45% versus 42%, P =.65). When results were categorized into presence or absence of high-risk HPV types, agreement between self- and clinician-collected specimens was high (kappa 0.72) and the difference between test results was not significant (McNemar's P =.41). However, when all HPV types detected were considered, agreement was perfect in only 51% of those with 1 or more types of high-risk HPV type. There was no association between agreement and age or HPV type.
  
  
  CONCLUSIONS
  Self testing for HPV DNA may be sufficiently sensitive for the detection of high-risk HPV DNA among adolescent and young adult women in clinical settings.
authors.affiliation: Division of Adolescent Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio 45229, USA. jessica.kahn@cchmc.org
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Kahn
authors.forename: Gail B
authors.initials: GB
authors.lastname: Slap
authors.forename: Bin
authors.initials: B
authors.lastname: Huang
authors.forename: Susan L
authors.initials: SL
authors.lastname: Rosenthal
authors.forename: Abbigail M
authors.initials: AM
authors.lastname: Wanchick
authors.forename: Linda M
authors.initials: LM
authors.lastname: Kollar
authors.forename: Paula A
authors.initials: PA
authors.lastname: Hillard
authors.forename: David
authors.initials: D
authors.lastname: Witte
authors.forename: Pam
authors.initials: P
authors.lastname: Groen
authors.forename: David I
authors.initials: DI
authors.lastname: Bernstein
mesh_terms: D000293:Adolescent; D000328:Adult; D015344:DNA Probes, HPV; D006801:Humans; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D011446:Prospective Studies; D015203:Reproducibility of Results; D013048:Specimen Handling / Q000379:methods*
chemical_list: D015344:DNA Probes, HPV