title: Chimerism and clinical outcomes of 110 recipients of unrelated donor bone marrow transplants who underwent conditioning with low-dose, single-exposure total body irradiation and cyclophosphamide.
abstract: We hypothesized that low-dose (550-cGy), single-exposure, high dose rate (30 cGy/min) total body irradiation (TBI) with cyclophosphamide as conditioning for HLA-compatible unrelated donor (URD) bone marrow transplantation (BMT) would result in donor chimerism (DC) with a low risk for serious organ toxicity and treatment-related mortality (TRM). Twenty-six patients with good risk diagnoses (acute leukemia in first complete remission [CR] and chronic-phase chronic myelogenous leukemia [CML]) and 84 with poor risk diagnoses underwent this regimen and URD BMT. Unsorted marrow nucleated cells were assessed for chimerism using VNTR probes. All DC occurred in 78 (86%) of 91 evaluable patients at 1 or more follow-up points. Graft failure occurred in 7 (7.7%) patients. Fatal organ toxicity occurred in only 2% of patients. TRM rates through 2 years of follow-up were 19% and 42% in those with good and poor risk diagnoses, respectively. Overall and disease-free survival rates in the good risk group were 47% and 40%, respectively, and in the poor risk group they were 25% and 21%, respectively, at a median follow-up for living patients of 850 days (range, 354-1588 days). This regimen resulted in 100% DC in most patients undergoing URD BMT with a relatively low risk for fatal organ toxicity and TRM.
authors.affiliation: Department of Internal Medicine, Division of Oncology, Section of Bone Marrow Transplantation and Leukemia, Washington University School of Medicine, St Louis, MO 63110-1093, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Girgis
authors.forename: Chris
authors.initials: C
authors.lastname: Hallemeier
authors.forename: William
authors.initials: W
authors.lastname: Blum
authors.forename: Randy
authors.initials: R
authors.lastname: Brown
authors.forename: Hsiu-San
authors.initials: HS
authors.lastname: Lin
authors.forename: Hanna
authors.initials: H
authors.lastname: Khoury
authors.forename: L Tim
authors.initials: LT
authors.lastname: Goodnough
authors.forename: Ravi
authors.initials: R
authors.lastname: Vij
authors.forename: Steve
authors.initials: S
authors.lastname: Devine
authors.forename: Marita
authors.initials: M
authors.lastname: Wehde
authors.forename: Stacey
authors.initials: S
authors.lastname: Postma
authors.forename: Aarti
authors.initials: A
authors.lastname: Oza
authors.forename: John
authors.initials: J
authors.lastname: Dipersio
authors.forename: Douglas
authors.initials: D
authors.lastname: Adkins
mesh_terms: D000328:Adult; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D003520:Cyclophosphamide / Q000008:administration & dosage*; D005334:Fever / Q000209:etiology / Q000401:mortality; D005500:Follow-Up Studies; D006085:Graft Survival; D006086:Graft vs Host Disease / Q000209:etiology / Q000401:mortality; D006650:Histocompatibility Testing; D006801:Humans; D007166:Immunosuppressive Agents / Q000008:administration & dosage*; D007239:Infections / Q000209:etiology / Q000401:mortality; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000401:mortality / Q000628:therapy*; D008875:Middle Aged; D020127:Recovery of Function / Q000276:immunology; D012008:Recurrence; D012307:Risk Factors; D018183:Transplantation Chimera; D019172:Transplantation Conditioning / Q000379:methods; D016896:Treatment Outcome; D014916:Whole-Body Irradiation
chemical_list: D007166:Immunosuppressive Agents; D003520:Cyclophosphamide