title: Fish as bioreactors: transgene expression of human coagulation factor VII in fish embryos.
abstract: A plasmid containing human coagulation factor VII (hFVII) complementary DNA regulated by a cytomegalovirus promoter was microinjected into fertilized eggs of zebrafish, African catfish, and tilapia. The active form of hFVll was detected in the fish embryos by various assays. This positive expression of human therapeutic protein in fish embryos demonstrates the possibility of exploitation of transgenic fish as bioreactors.
authors.affiliation: Division of Cell Sciences, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, SO16 7PX, UK.
authors.forename: Gyulin
authors.initials: G
authors.lastname: Hwang
authors.forename: Ferenc
authors.initials: F
authors.lastname: Müller
authors.forename: M Aziz
authors.initials: MA
authors.lastname: Rahman
authors.forename: Darren W
authors.initials: DW
authors.lastname: Williams
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Murdock
authors.forename: K John
authors.initials: KJ
authors.lastname: Pasi
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Goldspink
authors.forename: Hamid
authors.initials: H
authors.lastname: Farahmand
authors.forename: Norman
authors.initials: N
authors.lastname: Maclean
mesh_terms: D000818:Animals; D019149:Bioreactors; D001777:Blood Coagulation / Q000187:drug effects; D003587:Cytomegalovirus / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005167:Factor VII / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D005399:Fishes / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D008845:Microinjections; D010783:Photometry; D011401:Promoter Regions, Genetic / Q000235:genetics; D019076:Transgenes / Q000235:genetics*; D015053:Zygote / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D005167:Factor VII