title: Specific detection and identification of herpes B virus by a PCR-microplate hybridization assay.
abstract: Herpes B virus DNA was specifically amplified by PCR, targeting the regions that did not cross-react with herpes simplex virus (HSV). The amplified products, which were shown to be highly genetic polymorphisms among herpes B virus isolates, were identified by microplate hybridization with probes generated by PCR. The products immobilized in microplate wells were hybridized with the biotin-labeled probes derived from the SMHV strain of herpes B virus. The amplified products derived from the SMHV and E2490 strains of herpes B virus were identified by microplate hybridization. PCR products amplified from the trigeminal ganglia of seropositive cynomolgus macaques were identified as herpes B virus DNA. The utility of the PCR-microplate hybridization assay for genetic detection and identification of the polymorphic region of herpes B virus was determined.
authors.affiliation: Department of Veterinary Public Health, Nippon Veterinary and Animal Science University, Musashino, Tokyo 180-8602, Japan.
authors.forename: Chika
authors.initials: C
authors.lastname: Oya
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Ochiai
authors.forename: Yojiro
authors.initials: Y
authors.lastname: Taniuchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Takano
authors.forename: Fukiko
authors.initials: F
authors.lastname: Ueda
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yoshikawa
authors.forename: Ryo
authors.initials: R
authors.lastname: Hondo
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D006566:Herpesviridae Infections / Q000821:virology; D006568:Herpesvirus 1, Cercopithecine / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008252:Macaca fascicularis; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D012668:Trigeminal Ganglion / Q000821:virology
chemical_list: D017931:DNA Primers; D004279:DNA, Viral