title: Lipoxygenase inhibitory activity of octyl gallate.
abstract: Octyl gallate inhibited soybean lipoxygenase-1 (EC 1.13.11.12, type I) with an IC(50) of 1.3 microM. The inhibition of the enzyme by octyl gallate is a slow and reversible reaction without residual activity. The inhibition kinetics analyzed by Lineweaver-Burk plots indicates that octyl gallate is a competitive inhibitor, and the inhibition constant, K(I), was obtained as 0.54 microM. One molecule of octyl gallate scavenged six molecules of 1,1-diphenyl-2-picrylhydrazyl and inhibited autoxidative lipid peroxidation. In addition, octyl gallate was effective in preventing lipid peroxidation.
authors.affiliation: Department of Environmental Science, Policy and Management, University of California, Berkeley, California 94720-3112, USA.
authors.forename: Tae Joung
authors.initials: TJ
authors.lastname: Ha
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Nihei
authors.forename: Isao
authors.initials: I
authors.lastname: Kubo
mesh_terms: D001667:Binding, Competitive; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects; D008084:Lipoxygenase / Q000378:metabolism; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology*; D013025:Glycine max / Q000201:enzymology*
chemical_list: D016859:Lipoxygenase Inhibitors; C016627:octyl gallate; D005707:Gallic Acid; D008084:Lipoxygenase