[Comparison of apoptosis expression in the secretory endometrium during a normal menstrual cycle].
OBJECTIVE
Comparison of level of apoptosis in the mid-secretory human endometrium.


METHODS
Clinical-laboratory trial.


METHODS
Department of Gynecology and Obstetrics, Medical Faculty, Palacký University, Faculty Hospital, Olomouc, Department of Gynecology and Obstetrics, Bata Hospital, Zlín.


METHODS
Samples of secretory endometrium were obtained from 14 women. The women included in the study had the next criteria: a history of infertility of more than 12 months, regular menstrual cycles, age below 40 and FSH basal level range 5-9 IU/L. Sampling of tissue was performed after confirmed ovulation by LH surge and repeated ultrasound examinations. Patterns were processed by routine methods and apoptosis was detected using TUNEL assay.


RESULTS
The level of apoptosis in the endometrial epithelial surface was significantly higher on day +7 compared to day after ovulation (P < 0.02). Apoptotic cells were seen only sporadically in endometrial stromal cells. The level of apoptosis in endometrial stroma was also higher (but not statistically significant) on day +7 compared to day  The level of apoptosis was markedly higher in spongy layer on day +7 (P < 0.01) compared to day  There were no significant differences between day and +7 in compact layers of the glands.
Havelka
P
P
Gynekologicko-porodnické oddĕlení Batovy nemocnice, Zlín.
Oborná
I
I
Lichnovský
V
V
Krsková
M
M
Fingerová
H
H
Brezinová
J
J
D000328:Adult; D017209:Apoptosis; D004717:Endometrium / Q000166:cytology* / Q000473:pathology / Q000502:physiology; D004848:Epithelium / Q000473:pathology; D005260:Female; D006801:Humans; D020287:In Situ Nick-End Labeling; D007247:Infertility, Female / Q000473:pathology; D008183:Luteal Phase; D008597:Menstrual Cycle; D010060:Ovulation