title: Sequence-altered peptide adopts optimum conformation for modification-dependent binding of the yeast tRNAPhe anticodon domain.
abstract: Amino acid contributions to protein recognition of naturally modified RNAs are not understood. Circular dichroism spectra and predictive software suggested that peptide tF2 (S1ISPW5GFSGL10 LRWSY15), selected from a phage display library to bind the modified anticodon domain of yeast tRNAPhe (ASL), adopted a beta-sheet structure. Ala residues incorporated at positions Pro4 and Gly6, both predicted to be involved in a turn, did not alter the peptide binding affinity for the ASLPhe, although major changes in the peptide's CD spectra were observed. Substitutions at three positions Pro4, Gly6, and Gly9, the latter not predicted to be in a turn, reduced the peptide's binding affinity to 4% of that of the unsubstituted tF2 and strongly influenced the peptide's secondary structure. The results suggest that peptides with different conformations, but similar affinities, adopt the optimal binding conformation, indicative of a structurally adaptive model of binding in which the modified RNA serves as a scaffold.
authors.affiliation: Department of Chemistry, University of Gdansk, Sobieskiego 18, Gdansk 80-952, Poland. fly@chem.univ.gda.pl
authors.forename: Piotr
authors.initials: P
authors.lastname: Mucha
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Szyk
authors.forename: Piotr
authors.initials: P
authors.lastname: Rekowski
authors.forename: Paul F
authors.initials: PF
authors.lastname: Agris
mesh_terms: D000926:Anticodon / Q000737:chemistry*; D002942:Circular Dichroism; D008968:Molecular Conformation; D009843:Oligoribonucleotides / Q000737:chemistry*; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry*; D012331:RNA, Fungal / Q000737:chemistry*; D012360:RNA, Transfer, Phe / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*
chemical_list: D000926:Anticodon; D009843:Oligoribonucleotides; D019151:Peptide Library; D010455:Peptides; D012331:RNA, Fungal; D012360:RNA, Transfer, Phe