HPV typing in Brazilian patients with epidermodysplasia verruciformis: high prevalence of EV-HPV 25.
BACKGROUND
Epidermodysplasia verruciformis is a rare genetic disorder characterized by development of lesions associated with HPV#5 or HPV#8 in early childhood; malignant transformation occurs in approximately half of individuals during adulthood.


OBJECTIVE
Our goal was to study the presence and spectrum of EV-HPV types in Brazilian EV patients, a population that had never been studied in this regard.


METHODS
Forty-one biopsies from different lesions (benign and skin tumors) and one biopsy from clinically normal skin from each of 20 Brazilian patients with EV were studied for HPV typing using nested PCR.


RESULTS
EV-HPV DNA was detected in all 41 skin lesions of the patients and was also identified in specimens considered as normal skin from 8 patients (40%). In this study HPV-EV 25 was the most prevalent (70%), and HPV 14d (67%) was highly associated with malignant lesions.


CONCLUSIONS
EV-HPV 25 was the most prevalent in our study. The noteworthy association of EV-HPV type 14d with skin cancers suggests its possible oncogenic role in malignant transformation in this population.
de Oliveira
Walmar Roncalli P
WR
Department of Dermatology, University of São Paulo, São Paulo, Brazil.
He
Qin
Q
Rady
Peter L
PL
Hughes
Thomas K
TK
Neto
Cyro Festa
CF
Rivitti
Evandro A
EA
Tyring
Stephen K
SK
D000293:Adolescent; D000328:Adult; D001938:Brazil; D004819:Epidermodysplasia Verruciformis / Q000821:virology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics; D017422:Sequence Analysis, DNA; D012867:Skin / Q000821:virology; D012878:Skin Neoplasms / Q000821:virology