[Analysis of population genetic structure and molecular identification of Changium smyrnioides and Chuanminshen violaceum with ISSR marker].
OBJECTIVE
To assess the population genetic diversity and genetic structure and screen species-specific bands for identification of Changium smyrnioides and Chuanminshen violaceum.


METHODS
Seven wild populations of Changium smyrnioides and one cultivated population of Chuanminshen violaceum were studied by ISSR analysis. The population genetic diversity and population genetic structure were assessed by using POPGENE software.


RESULTS
A total of 152 ISSR markers were scored, among which 136 (90.8%) were polymorphic. The values of Gst tended to be high (mean Gst = 0.575). The level of genetic divesity of Changium smyrnioides (A = 1.272; P = 27.26%; I = 0.132; H = 0.087) was higher than that of Chuanminshen violaceum (A = 1.217; P = 21.7; I = 0.103; H = 0.067).


CONCLUSIONS
The genetic variation of Changium smyrnioides is high and the majority of genetic variation occur among populations. Substantial genetic divergence is shown by cluster analysis (UPGMA) to befound between Changium smyrnioides and Chuanminshen violaceum at DNA level. In addition, one species-specific marker has been obtained in Chuanminshen violaceum. The phylogenetic relationship of two species has also been discussed.
Qiu
Ying-xiong
YX
College of Life Sciences, Zhejiang University, Hangzhou 310029, Zhejiang, China.
Fu
Cheng-xin
CX
Wu
Fei-jie
FJ
D019661:Apiaceae / Q000145:classification / Q000235:genetics*; D002681:China; D016000:Cluster Analysis; D018744:DNA, Plant / Q000235:genetics*; D017753:Ecosystem; D005787:Gene Frequency; D005819:Genetic Markers; D040342:Genetic Structures; D010802:Phylogeny; D010946:Plants, Medicinal / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
D018744:DNA, Plant; D005819:Genetic Markers