Synthesis of fluorescein labeled 7-methylguanosinemonophosphate.
Binding of eIF4E to the cap structure (m(7)GpppN) plays a critical role in mRNA translation. To study the interaction between eIF4E and cap, and to identify small molecule inhibitors of their binding, we synthesized a fluorescent-labeled cap analogue and used it to develop a fluorescence-polarization assay. This preliminary communication describes the synthesis of a fluorescein labeled 7-methylguanosinemonophosphate, and its dose dependent binding to purified human eIF4E as demonstrated by the fluorescence polarization assay.
Natarajan
Amarnath
A
Laboratory for Translational Research, One Kendall Square, Building 600, 3rd Floor, Harvard Medical School, Cambridge, MA 02139, USA.
Moerke
Nathan
N
Fan
Yun-Hua
YH
Chen
Han
H
Christ
William J
WJ
Wagner
Gerhard
G
Halperin
Jose A
JA
D039561:Eukaryotic Initiation Factor-4E / Q000737:chemistry; D019793:Fluorescein; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes / Q000138:chemical synthesis; D006801:Humans; D011485:Protein Binding; D014176:Protein Biosynthesis; D012314:RNA Cap Analogs / Q000138:chemical synthesis* / Q000737:chemistry
D039561:Eukaryotic Initiation Factor-4E; D005456:Fluorescent Dyes; D012314:RNA Cap Analogs; C011925:7-methylguanosine-5'-monophosphate; D019793:Fluorescein