Radionuclide imaging of mesenchymal stem cells transplanted into spinal cord.
In vivo tracking of stem cells implanted to spinal cord by radionuclide imaging was investigated. The high expression of transferrin receptor on human mesenchymal stem cells (hMSCs) was verified by flow cytometry, radioligand binding and immunofluorescence. Radiolabelled transferrin was chosen as a tracer for scintigraphic imaging of the hMSCs transplanted into spinal cord of rabbits. Comparative experiments with radiolabelled human serum albumin as tracer and PBS as graft as well as ex vivo autoradiography demonstrated the specific uptake of radiolabelled transferrin of hMSCs. hMSCs could be detected in vivo with radiolabelled transferrin targeting at cellular transferrin receptors at an early stage after transplantation into spinal cord.
Bai
Jinzhu
J
Department of Orthopedics, Peking University Third Hospital, Beijing 100083, China.
Ding
Weimin
W
Yu
Minjun
M
Du
Jin
J
Liu
Zhongjun
Z
Jia
Bing
B
Li
Lingsong
L
Shen
Li
L
Tian
Jiahe
J
Wang
Fan
F
D000818:Animals; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000000981:diagnostic imaging*; D011817:Rabbits; D011877:Radionuclide Imaging; D013116:Spinal Cord / Q000000981:diagnostic imaging* / Q000637:transplantation*; D033581:Stem Cell Transplantation / Q000379:methods*