Removal of hepatitis A virus from water by polyacrylonitrile-based ultrafiltration membranes.
Ultrafiltration-based polyacrylonitrile membranes (UF-membranes) were evaluated for their ability to retain tissue culture adapted indigenous hepatitis A virus from water. Amicon cell (dead-end ultrafiltration unit)-based experiment was set up and viral assay was carried out using rapid and sensitive reverse transcriptase polymerase chain reaction (RT-PCR). The RT-PCR results show retention of virus particles by the polyacrylonitrile membranes. A protocol for routine virological evaluation of membranes is described and the use of these membranes for water purification units and for virus concentration systems for field application is discussed.
Vaidya
Sunil R
SR
Hepatitis Division, National Institute of Virology, 20-A Dr. Ambedkar road, Post Box No. 11, Pune 411001, India. suravaidya@yahoo.com
Kharul
Ulhas K
UK
Chitambar
Shobha D
SD
Wanjale
Santosh D
SD
Bhole
Yogesh S
YS
D000180:Acrylic Resins; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D030041:Hepatitis A virus / Q000235:genetics / Q000302:isolation & purification*; D008567:Membranes, Artificial; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014462:Ultrafiltration / Q000379:methods; D014773:Virology / Q000379:methods; D014871:Water Microbiology; D014881:Water Supply
D000180:Acrylic Resins; D004279:DNA, Viral; D008567:Membranes, Artificial; C010504:polyacrylonitrile