title: [Induction of IgG and IgM production by Epstein-Barr virus protein].
abstract: OBJECTIVE
  To detect the production of IgG and IgM by cultured umbilical blood B cell stimulated by UV and heat-inactivated Epstein-Barr virus (EBV).
  
  
  METHODS
  Mononuclear cells were isolated routinely from umbilical blood, then monocytes, NK cells and cytotoxic T cells were eliminated by L-leucine methy ester method; remained T cells were eliminated by AET-SRBC rosette method. The purified B cells were treated with UV or heat-inactivated EBV respectively and cultured in complete IMDM. The IgG and IgM in the supernatant were detected by ELISA.
  
  
  RESULTS
  The IgG and IgM production(A value) in UV-inactivated EBV group increased from 18th day to 30 th day after stimulation compared with the baseline. The A value of all time points in heat-inactivated EBV group had no significant difference.
  
  
  CONCLUSIONS
  UV-inactivated EBV may induce IgG and IgM production with a time-effect relationship, suggesting that EBV protein is a key component of stimulating Ig production. This result provide the basis for the further researching the effects of functional EBV protein on the production of natural autoantibodies (NAA).
authors.affiliation: Center of Dermatology of Chinese PLA, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yan-guo
authors.initials: YG
authors.lastname: Zhang
authors.forename: Meng
authors.initials: M
authors.lastname: Fu
authors.forename: Yu-fengg
authors.initials: YF
authors.lastname: Liu
authors.forename: Tian-wen
authors.initials: TW
authors.lastname: Gao
authors.forename: Lei
authors.initials: L
authors.lastname: Pan
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology / Q000821:virology; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology; D004854:Herpesvirus 4, Human / Q000528:radiation effects; D006358:Hot Temperature; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014764:Viral Proteins