Comparison of efficiency of ex vivo expansion of whole blood, mononuclear cells and purified CD34+ cells from human umbilical cord blood.
We used a dual-chamber culture system separated by a dialysis membrane to test the efficiency of expansion of whole cord blood and cell fractions. We found that expansion of progenitor cells was more efficient from whole blood than from purified CD34+ or partially purified mononuclear cell fractions.
Ryu
Kyung-Ha
KH
Shin
Hee-Young
HY
Ahn
Hyo-Seop
HS
Kim
Young-Ju
YJ
Woo
So-Youn
SY
Seoh
Ju-Young
JY
D018952:Antigens, CD34 / Q000097:blood*; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000276:immunology
D018952:Antigens, CD34