title: Properties of mycelial aggregate-specific lectin of Pleurotus cornucopiae produced in Pichia pastoris.
abstract: cDNA of a mycelial aggregate-specific lectin of Pleurotus cornucopiae was expressed in Pichia pastoris, and the expression product was purified and characterized. The product was functional, and the hemagglutinating activity was inhibited most strongly by the addition of N-acetyl-D-galactosamine as was the native lectin. The native lectin is a glycoprotein having five glycosylation recognition signals, and the expression product showed slightly larger molecular mass than that of the native one due to further glycosylation.
authors.affiliation: Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University, Matsudo, Japan.
authors.forename: Futoshi
authors.initials: F
authors.lastname: Sumisa
authors.forename: Naoto
authors.initials: N
authors.lastname: Iijima
authors.forename: Akikazu
authors.initials: A
authors.lastname: Ando
authors.forename: Maiko
authors.initials: M
authors.lastname: Shiga
authors.forename: Kozue
authors.initials: K
authors.lastname: Kondo
authors.forename: Koh
authors.initials: K
authors.lastname: Amano
authors.forename: Yoshiho
authors.initials: Y
authors.lastname: Nagata
mesh_terms: D050260:Carbohydrate Metabolism; D018076:DNA, Complementary / Q000235:genetics; D006031:Glycosylation; D006385:Hemagglutination Inhibition Tests; D037102:Lectins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D010843:Pichia / Q000235:genetics*; D020076:Pleurotus / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D018076:DNA, Complementary; D037102:Lectins; D011994:Recombinant Proteins