title: [Tissue microarrays. Value of immunohistochemical proliferation markers for serial investigations of head and neck cancer].
abstract: BACKGROUND
  Tissue microarrays (TMAs) are still seldom used in oncological research, especially that involving otorhinolaryngology, although the benefit of this method has often been reported.
  
  
  METHODS
  We prepared ten different tumor probes from patients with head and neck cancer for immunohistochemistry and performed ki67 and proliferating cell nuclear antigen (PCNA) staining on the usual paraffin sections as well as on TMAs consisting of the same tissue.
  
  
  CONCLUSIONS
  We conclude that investigation by means of TMA makes oncological research, and especially the screening of tumor probes, much more efficient since a high correlation ( r>0.7, P<0.0001) between both methods was found.
authors.affiliation: Zentrum für Hals-Nasen-Ohren-Heilkunde, Kopf- und Halschirurgie, Johann-Wolfgang-Goethe-Universität, Frankfurt am Main. silke.peters@em.uni-frankfurt.de
authors.forename: S
authors.initials: S
authors.lastname: Peters
authors.forename: M
authors.initials: M
authors.lastname: Hambek
authors.forename: W
authors.initials: W
authors.lastname: Gstöttner
authors.forename: R
authors.initials: R
authors.lastname: Knecht
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D006258:Head and Neck Neoplasms / Q000175:diagnosis*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D019394:Ki-67 Antigen / Q000032:analysis*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen