title: Voltammetry of osmium-modified DNA at a mercury film electrode: application in detecting DNA hybridization.
abstract: Mercury film electrodes (MFE) have recently been used in nucleic acid electrochemical analysis as alternatives to the classical mercury drop ones. DNA modified with osmium tetroxide, 2,2'-bipyridine (Os,bipy) can be detected with a high sensitivity at mercury electrodes via measurements of a catalytic osmium signal. In this paper we show that mercury film on a glassy carbon electrode can be used in voltammetric analysis of Os,bipy-modified DNA. Application of the MFE as a detection electrode in double-surface electrochemical DNA hybridization assay involving osmium labeling of target DNA is demonstrated.
authors.affiliation: Laboratory of Biophysical Chemistry and Molecular Oncology, Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, Brno 612 65, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Kostecka
authors.forename: Ludek
authors.initials: L
authors.lastname: Havran
authors.forename: Hana
authors.initials: H
authors.lastname: Pivonkova
authors.forename: Miroslav
authors.initials: M
authors.lastname: Fojta
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D008628:Mercury / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009942:Organometallic Compounds / Q000032:analysis / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D020099:Coated Materials, Biocompatible; D015342:DNA Probes; D009942:Organometallic Compounds; D015082:2,2'-Dipyridyl; C076491:osmium tetroxide-2,2'-bipyridine; D004247:DNA; C427203:calf thymus DNA; D008628:Mercury