Cloning and characterization of a mouse spergen-1 localized in sperm mitochondria.
Development of spermatozoa is a complex process involving specific morphological formation of flagella, nucleus and mitochondria. Although detailed morphological observations of these events are available, the molecular mechanisms remain to be fully elucidated. We report here the molecular cloning and characterization of mouse spergen-1 encoding a sperm specific mitochondrial protein, from a haploid germ cell-specific subtracted cDNA library of mouse testis. Isolated cDNA is c. 0.7 kb and contains a 465 bp ORF that encodes mouse spergen-1, a sperm mitochondrial protein consisting of 154 predicted amino acids. Antibodies raised against mouse Spergen-1 identified a testis-specific c. 18 Mr x 10(3) band in Western blot analysis. The protein was localized to the mitochondria of mouse sperm. Comparison of the mouse and human genomic sequences showed that 55 bps of the 5'-upstream region containing a CAAT box and binding sequence for NF-kappa B is conserved and could be important for specific expression of mouse spergen-1 in haploid germ cells.
Matsuoka
Yasuhiro
Y
Department of Science for Laboratory Animal Experimentation, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita City, Osaka 565 0871, Japan.
Iguchi
Naoko
N
Kitamura
Kouichi
K
Nishimura
Hiromi
H
Manabe
Hiroyuki
H
Miyagawa
Yasushi
Y
Koga
Minoru
M
Matsumiya
Kiyomi
K
Okuyama
Akihiko
A
Tanaka
Hiromitsu
H
Nishimune
Yoshitake
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D017124:Conserved Sequence; D015723:Gene Library; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000737:chemistry*; D024101:Mitochondrial Proteins / Q000737:chemistry* / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D029589:Seminal Plasma Proteins / Q000737:chemistry* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013094:Spermatozoa / Q000166:cytology*
D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D029589:Seminal Plasma Proteins; C494888:spergen-1 protein, mouse