Chitosanase activity in Bacillus thuringiensis.
The ability to produce extracellular chitosanase (EC 3.2.1.132) was found by plate assays in 18 (23%) out of 77 crystalliferous strains of Bacillus thuringiensis. The best chitosanase producer was selected after the growth chosen in a liquid medium with colloidal chitosan as carbon source. Enzyme production was optimized (a 4-d incubation at 32 degrees C with shaking in a medium of pH 6.5 with 4% colloidal chitosan) and the enzyme was partially characterized. This is the first report on the chitosanase of B. thuringiensis.
Cruz Camarillo
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Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politecnico National, México 11340, DF, México. cruzcamarillo@hotmail.com
Sánchez Pérez
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Rojas Avelizapa
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NG
Gómez Ramírez
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Rojas Avelizapa
L I
LI
D001413:Bacillus thuringiensis / Q000201:enzymology*; D002686:Chitin / Q000031:analogs & derivatives* / Q000378:metabolism; D048271:Chitosan; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004795:Enzyme Stability; D006026:Glycoside Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010572:Pest Control, Biological; D013696:Temperature
D004791:Enzyme Inhibitors; D002686:Chitin; D048271:Chitosan; D006026:Glycoside Hydrolases; C019868:chitosanase