Diagnosis of intestinal acariasis with avidin-biotin system enzyme-linked immunosorbent assay.
OBJECTIVE
To explore the value of avidin-biotin system enzyme-linked immunosorbent assay (ABC-ELISA) in diagnosis of intestinal acariasis.


METHODS
Mite-specific IgG levels in serum of 48 patients with intestinal acariasis were measured with ABC-ELISA. The sensitivity of this method was compared with that of staphylococcal protein A enzyme-linked immunosorbent assay (SPA-ELISA).


RESULTS
The positive rate of mite-specific IgG detected with ABC-ELISA and SPA-ELISA was 89.58% (43/48) and 56.25% (27/48), respectively. The positive rate with ABC-ELISA was statistically higher than that with SPA-ELISA (chi2=13.50, P<0.01).


CONCLUSIONS
ABC-ELISA is an effective method for the diagnosis of intestinal acariasis.
Zhang
Rong-Bo
RB
Department of Etiology and Immunology, School of Medicine, Anhui University of Science and Technology, Huainan 232001, Anhui Province, China.
Huang
Yong
Y
Li
Chao-Pin
CP
Cui
Yu-Bao
YB
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D008297:Male; D008924:Mite Infestations / Q000175:diagnosis*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity