Tissue CA-19.9 content in colorectal adenomas and its value in the assessment of dysplasia.
BACKGROUND
occasionally, the risk of malignant transformation may be difficult to establish in adenomatous polyps due to the fact that they contain areas with variable grades of dysplasia. A measurement of tissue tumor markers may be useful to recognize these adenomas.


OBJECTIVE
the aims of this study were: to established firstly the relationship between carbohydrate antigen 19.9 (CA-19.9) content in the colorectal mucosa and the characteristics of polyps, and secondly, the diagnostic value of the formers measurement.


METHODS
tissue CA-19.9 concentration was measured in 155 colorectal samples obtained from 145 patients (21 normal mucosa; 113 adenomatous polyps; 21 adenocarcinoma). Cytosol CA-19.9 content was determined by enzyme-linked immunoadsorbant assay, and the measurement of this protein was achieved by quantitative assay. Tissue samples were also processed for histological examination.


RESULTS
we demonstrated that CA-19.9 levels in adenomatous polyps and adenocarcinomas were significantly higher than in the normal mucosa. These levels varied significantly according to polyp size, histological type, and grade of dysplasia. CA-19.9 contents were higher in polyps with a high risk of malignant transformation than in those with a low risk of severe dysplasia. The cut-off value 214 U/mg of protein properly differentiated both types of risk. The area under the receiver operating characteristic (ROC) curves showed that cytosol CA-19.9 levels allow classifying polyps according to their histological features.


CONCLUSIONS
we concluded that the measurement of CA-19.9 content in adenomatous polyps may be useful to classify these tumors and confirm the feasibility to separate adenomas into two groups: low and high risk of malignant change.
Salces
I
I
Department of Digestive Diseases, Unit of Research: Research Center, Hospital 12 Octubre, Universidad Complutense, Madrid, Spain. luisframos@wanadoo.es
Vegh
I
I
Rodríguez-Muñoz
S
S
Colina
F
F
Pérez
A
A
Soto
S
S
Sánchez
F
F
de la Cruz
J
J
Solís-Herruzo
J A
JA
D000230:Adenocarcinoma / Q000737:chemistry* / Q000473:pathology; D000236:Adenoma / Q000737:chemistry* / Q000473:pathology; D018395:CA-19-9 Antigen / Q000032:analysis*; D003111:Colonic Polyps / Q000737:chemistry / Q000473:pathology; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D012720:Severity of Illness Index
D018395:CA-19-9 Antigen