Evidence of electroconformational changes in membrane proteins: field-induced reductions in intra membrane nonlinear charge movement currents.
Experimental results are presented to show that a pulsed, intensive membrane potential can reduce intra membrane, nonlinear charge movement currents, which are the voltage-sensors in the voltage-dependent membrane proteins and in the excitation-contraction coupling of skeletal muscle fibers. The results indicate a possible mechanism involved in electrical injury: dysfunctions of the voltage-dependent membrane proteins caused by electroconformational damages in their voltage-sensors.
Chen
Wei
W
Cellular and Molecular Biophysics, Department of Physics, University of South Florida, 4202 E. Fowler Avenue, PHY 114 Tampa, FL 33620, USA. wchen@chumal.cas.usf.edu
D000818:Animals; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000502:physiology* / Q000528:radiation effects*; D002478:Cells, Cultured; D004574:Electromagnetic Fields; D008564:Membrane Potentials / Q000502:physiology / Q000528:radiation effects*; D008565:Membrane Proteins / Q000502:physiology / Q000528:radiation effects; D018485:Muscle Fibers, Skeletal / Q000502:physiology* / Q000528:radiation effects*; D018482:Muscle, Skeletal / Q000502:physiology / Q000528:radiation effects; D024642:Potassium Channels, Voltage-Gated / Q000502:physiology* / Q000528:radiation effects*; D011487:Protein Conformation / Q000528:radiation effects; D011898:Ranidae; D012964:Sodium / Q000378:metabolism
D015220:Calcium Channels; D008565:Membrane Proteins; D024642:Potassium Channels, Voltage-Gated; D012964:Sodium; D002118:Calcium