-1 frameshifting at a CGA AAG hexanucleotide site is required for transposition of insertion sequence IS1222.
The discovery of programmed -1 frameshifting at the hexanucleotide shift site CGA_AAG, in addition to the classical X_XXY_YYZ heptanucleotide shift sequences, prompted a search for instances among eubacterial insertion sequence elements. IS1222 has a CGA_AAG shift site. A genetic analysis revealed that frameshifting at this site is required for transposition.
Mejlhede
Nina
N
Laboratoire de Microbiologie et Génétique Moléculaire, UMR5100 Centre National de la Recherche Scientifique et Université Paul Sabatier, Toulouse 31062, France.
Licznar
Patricia
P
Prère
Marie-Françoise
MF
Wills
Norma M
NM
Gesteland
Raymond F
RF
Atkins
John F
JF
Fayet
Olivier
O
D001483:Base Sequence; D004251:DNA Transposable Elements; D018965:Frameshifting, Ribosomal; D008969:Molecular Sequence Data; D020638:Rahnella / Q000235:genetics*
D004251:DNA Transposable Elements