Scale-up micropropagation of sweet basil (Ocimum basilicum L.) in an airlift bioreactor and accumulation of rosmarinic acid.
Nodal explants with lateral buds and leaf-derived suspension cultures of sweet basil, Ocimum basilicum L., were cultured in 5 l airlift bioreactors for three weeks, thereby increasing the fresh wt of suspension cultures 2.5-fold. Rosmarinic acid accumulated at 29 micromicrog g(-1) dry wt in the suspension culture but, for micropropagated plants, it reached 178 microg g(-1) dry wt.
Kintzios
Spiridon
S
Laboratory of Plant Physiology, Faculty of Agricultural Biotechnology Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece. skin@aua.gr
Kollias
Haralambos
H
Straitouris
Evangelos
E
Makri
Olga
O
D000975:Antioxidants / Q000378:metabolism*; D019149:Bioreactors; D002934:Cinnamates / Q000378:metabolism*; D053630:Depsides; D018649:Ocimum basilicum / Q000378:metabolism*; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism*; D000097123:Rosmarinic Acid
D000975:Antioxidants; D002934:Cinnamates; D053630:Depsides; D000097123:Rosmarinic Acid