Analysis of the S. pombe signalling scaffold protein Cdc11p reveals an essential role for the N-terminal domain in SIN signalling.
The initiation of cytokinesis in the fission yeast Schizosaccharomyces pombe is signalled by the septation initiation network (SIN). Signalling originates from the spindle pole body (SPB), where SIN proteins are anchored by a scaffold composed of cdc11p and sid4p. Cdc11p links the other SIN proteins to sid4p and the SPB. Homologues of cdc11p have been identified in Saccharomyes cerevisiae (Nud1p) and human cells (Centriolin). We have defined functional domains of cdc11p by analysis of deletion mutants. We demonstrate that the C-terminal end of cdc11p is necessary for SPB localisation. We also show that the N-terminal domain is necessary and sufficient for signal transduction, since tethering of this domain to the SPB will substitute for cdc11p in SIN function.
Krapp
Andrea
A
Cell Cycle Control Laboratory, Swiss Institute for Experimental Cancer Research (ISREC), 1066 Epalinges, Switzerland.
Cano
Elena
E
Simanis
Viesturs
V
D018797:Cell Cycle Proteins / Q000737:chemistry / Q000502:physiology*; D002455:Cell Division; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000502:physiology*; D003851:Deoxyribonucleases / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism; D017353:Gene Deletion; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D009154:Mutation; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D012568:Schizosaccharomyces / Q000378:metabolism*; D015398:Signal Transduction; D008941:Spindle Apparatus; D013696:Temperature; D012359:tRNA Methyltransferases
C099502:CDC11 protein, S cerevisiae; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D029721:Drosophila Proteins; D008164:Luminescent Proteins; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins; D012359:tRNA Methyltransferases; D017346:Protein Serine-Threonine Kinases; D003851:Deoxyribonucleases; C077501:TRM2 protein, S cerevisiae