Defocused transfer function for a partially coherent microscope and application to phase retrieval.
The defocused weak-object transfer function of a partially coherent bright-field microscope is calculated. For weak defocus, this can be expressed analytically. Use of this transfer function for phase restoration (quantitative phase retrieval) from images of weak mixed phase-amplitude objects is discussed.
Sheppard
Colin J R
CJ
Division of Bioengineering and Department of Diagnostic Radiology, National University of Singapore, Singapore 117576. colin@nus.edu.sg