3-Amino-1,4-dimethyl-5H-pyrido[4,3-b]indole induces apoptosis and necrosis with activation of different caspases in rat splenocytes.
A dietary carcinogen, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) at 20 microM activates caspase-3-like proteases as an apoptotic marker in rat splenocytes. The present study demonstrated 100 microM Trp-P-1 induced necrosis with activation of caspase-3-like proteases. The activation in necrosis and apoptosis resulted from the activation of caspase-9 and caspase-8, respectively. Thus, Trp-P-1 induces apoptosis and necrosis with the activation of different caspases.
Hashimoto
Takashi
T
Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, USA.
Sano
Takashi
T
Ito
Wakana
W
Kanazawa
Kazuki
K
Danno
Gen-ichi
G
Ashida
Hitoshi
H
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002243:Carbolines / Q000494:pharmacology*; D020169:Caspases / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D009336:Necrosis; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013154:Spleen / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism
D002243:Carbolines; D017382:Reactive Oxygen Species; D020169:Caspases; C019132:3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole