Auxins affected ginsenoside production and growth of hairy roots in Panax hybrid.
Hairy roots of interspecific hybrid ginseng (Panax ginseng x P. quinquefolium), induced by Agrobacterium rhizogenes ATCC 15834, grew well in B5 liquid media supplemented with 2.5 microM auxins (3-indole butyric acid (IBA), 1-naphtaleneacetic acid (NAA) and 3-indoleacetic acid (IAA)). The hairy roots cultured in B5 liquid medium supplemented with 2.5 microM IBA showed best growth (6.39 g fresh weight per a flask, at week 8). The highest content of the total ginsenosides was 1.63% as dry weight at week 8 when cultured with 2.5 microM NAA. The different auxins affected the numbers and lateral branching roots. Especially, 2.5 microM IBA promoted the lateral root formation (43.7+/-4.0 roots, at week 8), and 2.5 microM NAA promoted the lateral root growth (45.3+/-5.6 mm, at week 8). The growth and ginsenosides production of 8-week old hairy roots cultured in B5 liquid media supplemented with IBA and NAA combinations were also investigated. Hairy roots produced higher amounts of ginsenosides in B5 liquid media supplemented with 0.5-1.0 microM IBA and NAA combinations than that cultured in B5 liquid media supplemented with only IBA and NAA. The highest yield of ginsenoside was obtained when cultured with 0.5 microM IBA and 1.0 microM IBA combination (6.38 mg per a flask, at week 8).
Washida
Daisuke
D
College of Pharmacy, Nihon University, Funabashi, Chiba, Japan. kitanaka@pha.nihon-u.ac.jp
Shimomura
Koichiro
K
Takido
Michio
M
Kitanaka
Susumu
S
D046508:Culture Techniques / Q000379:methods; D036145:Ginsenosides / Q000494:pharmacology*; D007210:Indoleacetic Acids / Q000494:pharmacology*; D005894:Panax / Q000187:drug effects* / Q000254:growth & development; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development
D036145:Ginsenosides; D007210:Indoleacetic Acids