title: Binding interaction studies of the immobilized Salmonella typhimurium with extracellular matrix and muscle proteins, and polysaccharides.
abstract: Our research attempts to understand the real-time interactions of immobilized Salmonella typhimurium with extracellular membrane proteins (collagen I, fibronectin and laminin) and muscle proteins (actin and myosin). Salmonella cells were immobilized on the sensor chip of a surface plasmon resonance (SPR) biosensor. Typical results showed that collagen I and myosin had higher binding responses to the S. typhimurium surface but laminin, actin and fibronectin had lower binding responses. The binding kinetics of collagen I and Salmonella cell surface showed an apparent dissociation and association rate constants of 3.90 E-4 s(-1) and 1.07 E+4 mol(-1) s(-1). Using the model system developed in our laboratory, the interactions of carrageenans and other polysaccharides with collagen and the Salmonella sensor surface were evaluated. The kappa-carrageenans blocked 92-100% binding of collagen to the Salmonella surface, while sodium alginate and low methoxy pectin blocked 50% and 18% binding, respectively. These biosensor studies allowed the rapid evaluation of compounds that may prevent bacterial attachment to poultry skin and carcasses, thus reducing pathogen contamination of poultry foods.
authors.affiliation: Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA. mmedina@arserrc.gov
authors.forename: Marjorie B
authors.initials: MB
authors.lastname: Medina
mesh_terms: D000199:Actins / Q000037:antagonists & inhibitors / Q000378:metabolism; D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D015374:Biosensing Techniques; D002351:Carrageenan / Q000494:pharmacology; D018914:Cells, Immobilized; D002645:Chickens; D003094:Collagen / Q000037:antagonists & inhibitors / Q000378:metabolism; D005353:Fibronectins / Q000037:antagonists & inhibitors / Q000378:metabolism; D005506:Food Contamination / Q000517:prevention & control*; D006801:Humans; D007700:Kinetics; D007797:Laminin / Q000037:antagonists & inhibitors / Q000378:metabolism; D009218:Myosins / Q000037:antagonists & inhibitors / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology; D011485:Protein Binding / Q000502:physiology*; D012486:Salmonella typhimurium / Q000502:physiology*; D020349:Surface Plasmon Resonance / Q000379:methods
chemical_list: D000199:Actins; D005353:Fibronectins; D007797:Laminin; D011134:Polysaccharides; D002351:Carrageenan; D003094:Collagen; D009218:Myosins