Boron nutrition of cultured tobacco BY-2 cells. IV. Genes induced under low boron supply.
Genes whose expression was up-regulated in low boron (B)-acclimated tobacco BY-2 (Nicotiana tabacum L. cv. Bright Yellow 2) cells, which had been selected under a low supply of B, were screened by the cDNA differential subtraction method. Thirteen genes were identified, including early salicylate-inducible glucosyltransferase, glutamine synthetase, glutathione S-transferase, and a pathogenesis-related protein, which might constitute a rescue system for oxidative damage. This indicates that B deficiency might impose cellular redox imbalance on the cells. Two of the 13 genes were induced within 30 min of B removal in the parent cells, indicating fast signal transfer from the cell walls to the cytoplasm.
Kobayashi
Masaru
M
Laboratory of Plant Nutrition, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
Mutoh
Takashi
T
Matoh
Toru
T
D000064:Acclimatization / Q000187:drug effects / Q000235:genetics*; D001895:Boron / Q000172:deficiency / Q000494:pharmacology*; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000235:genetics / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000235:genetics; D012459:Salicylates / Q000494:pharmacology; D015398:Signal Transduction / Q000235:genetics; D014026:Nicotiana / Q000166:cytology / Q000187:drug effects / Q000235:genetics*
D018076:DNA, Complementary; D012459:Salicylates; D005964:Glucosyltransferases; D005982:Glutathione Transferase; D005974:Glutamate-Ammonia Ligase; D001895:Boron