title: [Preparation and simultaneous determination of corynoline and acetylcorynoline in the herb of Corydalis bungeana].
abstract: OBJECTIVE
  To isolate and purify corynoline and acetylcorynoline from Corydalis bungeana and develop a reversed-phase HPLC method of determining the two components in C. bungeana.
  
  
  METHODS
  Alkaloids were isolated from the ethanolic extract with column gel chromatography, and identified on the basis of spectral analysis (UV, 1H-NMR, 13C-NMR) and physicochemical properties. For quantitative analysis of the two components, samples were separated on an ODS column with mobile phase of methanol-15 mmol.L-1 potassium dihydrogen phosphate/potassium phosphate dibasic (pH 6.70, 70:30). The flow rate was 0.8 mL.min-1, and the detection was set at 289 nm.
  
  
  RESULTS
  The purity was 99.5% and 99.1% for corynoline and acetylcorynoline respectively. The calibration curves were linear in the range of 6.9-110.4 mg.L-1 corynoline and 8.7-139.5 mg.L-1 acetylcorynoline. The RSD was 2.1% and 2.7%, and the average recovery was 97.3% and 97.2% respectively.
  
  
  CONCLUSIONS
  The method of isolating and purifying corynoline and acetylcorynoline from Corydalis bungeana and the HPLC method of simultaneous determination of the two components have been developed. The HPLC method is simple, easy to perform and applicable to the content determination of corynoline and acetylcorynoline in C. bungeana of various origins.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, Liaoning, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Huang
authors.forename: Hong-juan
authors.initials: HJ
authors.lastname: Yang
authors.forename: Fa-mei
authors.initials: FM
authors.lastname: Li
mesh_terms: D001600:Berberine Alkaloids / Q000032:analysis / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D031311:Corydalis / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D001600:Berberine Alkaloids; C487645:acetylcorynoline; C487644:corynoline