title: Preparation of protein gradients through the controlled deposition of protein-nanoparticle conjugates onto functionalized surfaces.
abstract: This paper describes a simple method for the preparation and characterization of protein density gradients on solid supports. The method employs colloidal metal nanoparticles as protein carriers and optical tags and is capable of forming linear, exponential, 1D, 2D, and multiprotein gradients of varying slope without expensive or sophisticated surface patterning techniques. Surfaces patterned with proteins using the procedures described within are shown to support cell growth and are thus suitable for studies of protein-cell interactions.
authors.affiliation: Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA.
authors.forename: Stephan
authors.initials: S
authors.lastname: Krämer
authors.forename: Huan
authors.initials: H
authors.lastname: Xie
authors.forename: John
authors.initials: J
authors.lastname: Gaff
authors.forename: John R
authors.initials: JR
authors.lastname: Williamson
authors.forename: Alexander G
authors.initials: AG
authors.lastname: Tkachenko
authors.forename: Navid
authors.initials: N
authors.lastname: Nouri
authors.forename: David A
authors.initials: DA
authors.lastname: Feldheim
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Feldheim
mesh_terms: D000818:Animals; D001369:Axons / Q000187:drug effects / Q000502:physiology; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D017979:Gold Colloid / Q000737:chemistry / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology; D008670:Metals / Q000737:chemistry; D008856:Microscopy, Fluorescence; D036103:Nanotechnology; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D011107:Polylysine / Q000737:chemistry; D051381:Rats; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000494:pharmacology*; D013053:Spectrophotometry
chemical_list: D017979:Gold Colloid; D008670:Metals; D011107:Polylysine; D012710:Serum Albumin, Bovine