Further method development for measurement of linezolid in human serum by MEKC.
A method for determining linezolid concentration in human serum using micellar electrokinetic capillary chromatography by direct injection of serum is described. A borate buffer (pH 8.0) containing sodium dodecyl sulfate was used as a run buffer and detection of linezolid was performed at 250 nm (its absorption maximum). The migration time of linezolid was 5.5 min and the detection limit was 0.5 mg/l (S/N = 3). The precision and accuracy of this method was good with no interference with the detection from bilirubin, hemoglobin and chyle of high concentrations. This provides a simple and easy method where samples of micro-quantity are used.
Kitahashi
Toshihiro
T
Department of Laboratory Medicine, Kinki University School of Medicine, 377-2 Ohno-higashi, Osakasayama, Osaka, Japan. kitahasi@med.kindai.ac.jp
Furuta
Itaru
I
D000081:Acetamides / Q000097:blood* / Q000737:chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods* / Q000639:trends; D006801:Humans; D000069349:Linezolid; D023303:Oxazolidinones / Q000097:blood* / Q000737:chemistry
D000081:Acetamides; D023303:Oxazolidinones; D000069349:Linezolid