[Construction and expression of prokaryotic expression vector for pTAT-HBV targeted ribonuclease fusion protein].
OBJECTIVE
To construct Tat-HBV targeted ribonuclease fusion protein prokaryotic expression vector and express it in E.coli.


METHODS
The cDNAs encoding HBV targeted ribonuclease, human eosinophil-derived neurotoxin and HBV core protein were respectively cloned into prokaryotic expression vector pTAT-HA. Recombinant plasmids were transformed into E.coli BL21(DE3) LysS, then the transformed cells were induced with IPTG. The expression of the fusion proteins were analyzed by SDS-PAGE and Western blot.


RESULTS
The three recombinant plasmids were constructed and expressed after IPTG induction successfully.


CONCLUSIONS
The obtained Tat-HBV targeted ribonuclease fusion protein has laid the foundation for using TR in therapy of HBV infection.
Ding
Jin
J
Department of Etiology, Fourth Military Medical University, Xi'an 710032, China.
Liu
Jun
J
Xue
Cai-fang
CF
Li
Ying-hui
YH
Gong
Wei-dong
WD
D047208:Eosinophil-Derived Neurotoxin / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D015696:Gene Products, tat / Q000235:genetics* / Q000378:metabolism; D015756:Genes, tat; D005822:Genetic Vectors; D006515:Hepatitis B virus / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012260:Ribonucleases / Q000235:genetics* / Q000378:metabolism; D014758:Viral Core Proteins / Q000235:genetics* / Q000378:metabolism
D015696:Gene Products, tat; D011993:Recombinant Fusion Proteins; D014758:Viral Core Proteins; D047208:Eosinophil-Derived Neurotoxin; D012260:Ribonucleases