title: Quantifying intermediates in template-directed natural product biosynthesis.
abstract: High-performance mass spectrometry is providing new experimental windows into the enzymology of natural product biosynthesis. The first quantitative assessments of covalently attached biosynthetic intermediates promise to shine new light on template-directed biosynthesis.
authors.affiliation: Departments of Chemistry and Biochemistry, University of Wisconsin, Madison, 1101 University Avenue, Madison, WI 53706, USA.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Belshaw
mesh_terms: D001665:Binding Sites; D001685:Biological Factors / Q000096:biosynthesis / Q000737:chemistry; D034261:Epothilones / Q000096:biosynthesis* / Q000737:chemistry*; D004952:Esters / Q000737:chemistry*; D013058:Mass Spectrometry; D009097:Multienzyme Complexes / Q000737:chemistry / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D001685:Biological Factors; D034261:Epothilones; D004952:Esters; D009097:Multienzyme Complexes; D013438:Sulfhydryl Compounds