[Modeling of acute respiratory distress syndrome in canine after inhalation of perfluoroisobutylene and preliminary study on mechanisms of injury].
OBJECTIVE
To establish of acute respiratory distress syndrome (ARDS) model in canine after inhalation of perfluoroisobutylene (PFIB), and to observe the progressing of lung injury, and to study the mechanisms of injury.


METHODS
A device of inhalation of PFIB for canine was made. The concentration of PFIB was 0.30 - 0.32 mg/L. Serum IL-6 and IL-8 were dynamically measured. Clinical manifestations, pathology of organs in canine were observed.


RESULTS
(1) During inhalation, the concentration of PFIB remained stable; (2) After inhalation, blood arterial oxygen partial pressure fell gradually, and eventually met the criteria for diagnosing ARDS; (3) The level of IL-8 in serum rises significantly after inhalation (P < 0.05), whereas that of IL-6 was not obviously altered (P > 0.05); (4) Within 6 hours after inhalation, no abnormality in canine was observed, but afterwards symptoms gradually appeared, and typical breath of ARDS, such as high frequency and lower level could be seen in later phase; (5) Pathological examination showed severe congestion, edema and atelectasis in most part of both lungs, and signs of anoxia in other organs.


CONCLUSIONS
(1) The device designed is capable of ensuring control of inhalation of PFIB; (2) Exposure to PFIB for 30 mins, canines all met the criteria for diagnosing ARDS 22 hours after inhalation, therefore the modeling is successful; (3) PFIB specifically damages the lung by causing excessive inflammation.
Liang
Hai-long
HL
Department of Cardiovascular Surgery, General Hospital of PLA, Beijing 100083, China.
Jiang
Chao-guang
CG
Zhang
Xian-cheng
XC
Ding
Ri-gao
RG
Huang
Chun-qian
CQ
Sun
Xiao-hong
XH
Hou
Ting-kui
TK
D000280:Administration, Inhalation; D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D005466:Fluorocarbons / Q000633:toxicity*; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D008168:Lung / Q000187:drug effects / Q000473:pathology*; D008297:Male; D011897:Random Allocation; D012128:Respiratory Distress Syndrome / Q000097:blood / Q000139:chemically induced*
D005466:Fluorocarbons; D015850:Interleukin-6; D016209:Interleukin-8; C016648:perfluoroisobutylene