Flow cytometry identification of X- and Y-chromosome-bearing goat spermatozoa.
Flow cytometric sorting technology was used to measure the difference in DNA content between X- and Y-chromosome-bearing spermatozoa in bucks. Spermatozoa were analysed by flow cytometry to characterize X- and Y-chromosome-bearing sperm populations and to quantify the DNA difference between them. Two symmetrical, overlapping and clearly separated peaks, corresponding to X- and Y-bearing spermatozoa, were detected. The difference in fluorescence intensity between the peaks was 4.4 +/- 0.03% without any significant inter- or intra-animal variations. Therefore, the identification and selection of high-purity samples of sperm populations for sex sorting is easier in bucks compared with other domestic species.
Parrilla
I
I
Department of Animal Medicine and Surgery (Animal Reproduction), University of Murcia, Murcia, Spain. parrilla@um.es
Vazquez
J M
JM
Roca
J
J
Martinez
E A
EA
D000818:Animals; D005434:Flow Cytometry / Q000379:methods / Q000662:veterinary*; D006041:Goats / Q000502:physiology*; D008297:Male; D012743:Sex Preselection / Q000379:methods / Q000662:veterinary*; D013094:Spermatozoa / Q000166:cytology*; D014960:X Chromosome / Q000235:genetics; D014998:Y Chromosome / Q000235:genetics