title: Keratin 2e: a marker for murine nipple epidermis.
abstract: Mesenchyme-derived signals influence the unique keratinization and appendage formation programs in specialized skin regions. Interactions between primary mammary mesenchyme and epidermal cells result in the formation of the nipple; however, it is unclear whether this represents a site of regionally specialized epidermis. We profiled the ultrastructure and keratin expression of the murine nipple, and the ventral skin of the K14-parathyroid hormone-related protein (PTHrP) transgenic mouse, which models nipple formation. We found the murine nipple and ventral K14-PTHrP epidermis display expanded suprabasal and granular layers, as well as a thickened cornified layer compared to ventral skin of wild-type littermates. We also observed increased levels of filaggrin in extracts from the ventral epidermis of the K14-PTHrP mouse when compared to that of wild-type littermates. Keratin 2e, previously reported to be expressed in various specialized epidermal sites in the mouse, is expressed in the nipple and the ventral skin of the K14-PTHrP mouse. Keratinocytes grown from the ventral epidermis of the K14-PTHrP mouse or wild-type littermates exhibited identical expression of epidermal markers in vitro, suggesting that the modulated differentiation profile observed in the nipple or the ventral K14-PTHrP skin was dependent on interactions with fibroblasts. The lack of appendages, altered stratification pattern and expression of a specialized keratin suggests that the murine nipple is an example of regionally specialized epidermis.
authors.affiliation: Medical Sciences, Indiana University School of Medicine, Bloomington, IN 47405, USA.
authors.forename: Bryon
authors.initials: B
authors.lastname: Mahler
authors.forename: Todd
authors.initials: T
authors.lastname: Gocken
authors.forename: Marija
authors.initials: M
authors.lastname: Brojan
authors.forename: Sue
authors.initials: S
authors.lastname: Childress
authors.forename: Dan F
authors.initials: DF
authors.lastname: Spandau
authors.forename: John
authors.initials: J
authors.lastname: Foley
mesh_terms: D000818:Animals; D015415:Biomarkers; D004586:Electrophoresis; D004817:Epidermis / Q000648:ultrastructure*; D005260:Female; D007150:Immunohistochemistry; D007633:Keratins; D051379:Mice; D008854:Microscopy, Electron; D009558:Nipples / Q000648:ultrastructure*
chemical_list: D015415:Biomarkers; D007633:Keratins