title: Presence of bacterial phage-like DNA sequences in commercial Taq DNA polymerase reagents.
abstract: Many studies have reported the presence of bacterial DNA contamination in commercial Taq DNA polymerase reagents. This is the first report of the presence of phage-like DNA sequences in certain commercial Taq DNA polymerase reagents. Precautions are needed when using amplification reagents with exogenous DNAs.
authors.affiliation: Department of Infectious and Parasitic Disease Pathology, American Registry of Pathology, Washington, DC, USA.
authors.forename: Tamara
authors.initials: T
authors.lastname: Newsome
authors.forename: Bing-Jie
authors.initials: BJ
authors.lastname: Li
authors.forename: Nianxiang
authors.initials: N
authors.lastname: Zou
authors.forename: Shyh-Ching
authors.initials: SC
authors.lastname: Lo
mesh_terms: D001435:Bacteriophages / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification*; D004340:Drug Contamination; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D019914:Taq Polymerase
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D007202:Indicators and Reagents; D019914:Taq Polymerase