title: Detection of Candida albicans mRNA in archival histopathology samples by reverse transcription-PCR.
abstract: The feasibility of detecting Candida albicans mRNA in formalin-fixed paraffin-embedded archival human histopathology specimens by reverse transcription-PCR (RT-PCR) was investigated. RT with gene-specific primers was used to detect five single-copy C. albicans gene transcripts, including those of two housekeeping genes, in oral candidiasis samples up to 8 years of age.
authors.affiliation: Department of Oral Sciences, School of Dentistry, University of Otago, Dunedin 9001, New Zealand.
authors.forename: Kyle T
authors.initials: KT
authors.lastname: Beggs
authors.forename: Ann R
authors.initials: AR
authors.lastname: Holmes
authors.forename: Richard D
authors.initials: RD
authors.lastname: Cannon
authors.forename: Alison M
authors.initials: AM
authors.lastname: Rich
mesh_terms: D001483:Base Sequence; D002176:Candida albicans / Q000235:genetics* / Q000302:isolation & purification; D002180:Candidiasis, Oral / Q000175:diagnosis / Q000473:pathology / Q000821:virology; D017931:DNA Primers / Q000235:genetics; D005557:Formaldehyde; D018628:Gene Dosage; D005800:Genes, Fungal; D006801:Humans; D016612:Paraffin Embedding; D012331:RNA, Fungal / Q000235:genetics* / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D016707:Tissue Fixation; D014773:Virology / Q000379:methods
chemical_list: D017931:DNA Primers; D012331:RNA, Fungal; D012333:RNA, Messenger; D005557:Formaldehyde