title: Plastic encapsulation of stabilized Escherichia coli and Pseudomonas putida.
abstract: Escherichia coli and Pseudomonas putida dried in hydroxyectoine or trehalose are shown to be highly resistant to the organic solvents chloroform and acetone, and consequently, they can be encapsulated in a viable form in solid plastic materials. Bacteria are recovered by rehydration after physical disruption of the plastic. P. putida incorporated into a plastic coating of maize seeds was shown to colonize roots efficiently after germination.
authors.affiliation: Institute of Biotechnology, University of Cambridge, Cambridge CB2 1QT, United Kingdom.
authors.forename: M
authors.initials: M
authors.lastname: Manzanera
authors.forename: S
authors.initials: S
authors.lastname: Vilchez
authors.forename: A
authors.initials: A
authors.lastname: Tunnacliffe
mesh_terms: D000096:Acetone / Q000494:pharmacology; D000599:Amino Acids, Diamino; D001709:Biotechnology / Q000379:methods*; D002725:Chloroform / Q000494:pharmacology; D003890:Desiccation; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development*; D010969:Plastics; D011137:Polystyrenes; D016958:Pseudomonas putida / Q000187:drug effects / Q000254:growth & development*; D012639:Seeds / Q000382:microbiology; D003313:Zea mays / Q000382:microbiology
chemical_list: D000599:Amino Acids, Diamino; D010969:Plastics; D011137:Polystyrenes; C121557:hydroxyectoine; D000096:Acetone; D002725:Chloroform