Direct optical detection of singlet oxygen from a single cell.
Singlet oxygen has been detected in single nerve cells by its weak 1270 nm phosphorescence (a1deltag --> X3sigmag-) upon irradiation of a photosensitizer incorporated in the cell. Thus, one can now consider the application of direct optical imaging techniques to mechanistic studies of singlet oxygen at the single-cell level.
Zebger
Ingo
I
Department of Chemistry, University of Aarhus, Arhus, Denmark.
Snyder
John W
JW
Andersen
Lars Klembt
LK
Poulsen
Lars
L
Gao
Zhan
Z
Lambert
John D C
JD
Kristiansen
Uffe
U
Ogilby
Peter R
PR
D000818:Animals; D006624:Hippocampus / Q000737:chemistry; D049449:Luminescence; D008433:Mathematics; D009474:Neurons / Q000737:chemistry*; D010777:Photochemistry; D017319:Photosensitizing Agents / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D026082:Singlet Oxygen / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods
D017319:Photosensitizing Agents; D011166:Porphyrins; D026082:Singlet Oxygen; C021096:tetra(4-N-methylpyridyl)porphine