Transient suppression of IgG1 with IL-6 over-expression in immunized TCR-transgenic mice.
Helper T cell-derived cytokines play a pivotal role in the production of antigen-specific IgG antibody by B cells. In order to examine the in vivo effect of massive activation of helper T cells on the production of specific antibodies, ovalbumin (OVA)-specific TCR transgenic mice (OVA23-3) were immunized with OVA and serum levels of antigen-specific antibodies were measured. As a result, a great enhancement of antigen-specific IgM secretion and delay of IgG secretion were observed while the T cells produced sufficient Th2 cytokines. Immediately after OVA-immunization, marked increase of serum IL-6 was noted, which was followed by a transient increase of antibody forming cells. Both in vivo and in vitro experiments demonstrated that excess amount of IL-6 enhanced IgM production by activated B cells while suppressing IgG production. These results suggest that overproduction of IL-6 in the early stages of the primary immunization promotes development of IgM producing cells and causes the delay of IgG1 secretion.
Kametani
Yoshie
Y
Division of Immunology, Department of Immunology, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan.
Katano
Ikumi
I
Hirano
Yasuyuki
Y
Mochida
Naoko
N
Takei
Eriko
E
Habu
Sonoko
S
D000818:Animals; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism*; D015850:Interleukin-6 / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D011948:Receptors, Antigen, T-Cell / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D013997:Time Factors
D007074:Immunoglobulin G; D015850:Interleukin-6; D011948:Receptors, Antigen, T-Cell