Hydrophobic interaction chromatography at low salt concentration for the capture of monoclonal antibodies.
We evaluated hydrophobic interaction chromatography (HIC) at low salt concentration for the capture of proteins from feed stocks by using monoclonal antibodies as model samples. It was indicated that the HIC at low salt concentration on critical hydrophobicity supports has a potential for capturing hydrophobic monoclonal antibodies directly from large volumes of feed stocks and recovering bound monoclonal antibodies in high yield. On the other hand, the HIC at low salt concentration did not seem so useful for the capture of weakly hydrophobic monoclonal antibodies. The recovery of weakly hydrophobic monoclonal antibodies from columns packed with critical hydrophobicity supports was not quantitative and significantly decreased as the residence time of the monoclonal antibodies in the columns became longer.
Kato
Yoshio
Y
Nanyo Research Laboratory, Tosoh Corporation, Kaisei-cho 4560, Shunan, Yamaguchi 746-8501, Japan. katoh_y@tosoh.co.jp
Nakamura
Koji
K
Kitamura
Takashi
T
Hasegawa
Masazumi
M
Sasaki
Hiroo
H
D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002850:Chromatography, Gel / Q000379:methods*; D009994:Osmolar Concentration
D000911:Antibodies, Monoclonal