Measurement of one-bond heteronuclear dipolar coupling contributions for amine and diastereotopic methylene protons.
One-bond heteronuclear and two-bond homonuclear residual dipolar couplings measured at methylene or amine sites can be utilized as long-range constraints in structure determination of molecules as well as to facilitate characterization of local conformation by stereospecific assignment of diastereotopic protons. We present two J-modulated HMQC type experiments to measure the one-bond heteronuclear dipolar coupling contributions of geminal protons individually. In addition two-bond homonuclear residual dipolar couplings between the diastereotopic protons are also obtained.
Kövér
Katalin E
KE
Department of Inorganic and Analytical Chemistry, University of Debrecen, Egyetem tér 1, P.O. Box 21, H-4010 Debrecen, Hungary. kover@tigris.unideb.hu
Fehér
Krisztina
K
D000465:Algorithms; D000588:Amines / Q000737:chemistry*; D001665:Binding Sites; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000737:chemistry; D006574:Heterocyclic Compounds, 2-Ring / Q000737:chemistry; D006838:Hydrocarbons; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008697:Methane / Q000031:analogs & derivatives* / Q000737:chemistry*; D008968:Molecular Conformation; D011522:Protons; D013237:Stereoisomerism
C439754:4,6-O-ethylideneglucopyranosylamine; D000588:Amines; D006574:Heterocyclic Compounds, 2-Ring; D006838:Hydrocarbons; D046911:Macromolecular Substances; D011522:Protons; C030011:carbene; D005944:Glucosamine; D008697:Methane