Recombinant single-chain canine interleukin 12 induces interferon gamma mRNA expression in peripheral blood mononuclear cells of dogs with visceral leishmaniasis.
Canine visceral leishmaniasis poses important concerns for public health and veterinary medicine in many areas of the world. Resistance to it seems to be associated with cellular specific immune responses of the so-called Th1 type. Interleukin-12 (IL-12) is one of the most potent inducers of Th1 type of immune responses to co-administered antigens. Herein, the cloning of canine IL-12, as a single-chain fusion protein (sccaIL-12), and its expression in biologically active form in COS-7 cells is reported. Supernatants from these cells stimulated the expression of comparable amounts of interferon gamma mRNA in peripheral blood mononuclear cells from dogs with natural visceral leishmaniasis. In addition, after stimulation with sccaIL-12, there was no difference between interferon gamma mRNA expressions in peripheral blood mononuclear cells of dogs with visceral leishmaniasis and from normal healthy control animals.
dos Santos
Lenita Ramires
LR
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Rua Waldemar Falcão, No. 121, Brotas, Salvador-Bahia, CEP 40295-001, Brazil.
Barrouin-Melo
Stella Maria
SM
Chang
Yung-Fu
YF
Olsen
John
J
McDonough
Sean P
SP
Quimby
Fred
F
dos Santos
Washington Luis Conrado
WL
Pontes-de-Carvalho
Lain Carlos
LC
Oliveira
Geraldo Gileno de Sá
GG
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004283:Dog Diseases / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology; D004285:Dogs; D015870:Gene Expression; D066298:In Vitro Techniques; D007369:Interferon Inducers / Q000737:chemistry / Q000494:pharmacology*; D007371:Interferon-gamma / Q000235:genetics*; D018664:Interleukin-12 / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D007898:Leishmaniasis, Visceral / Q000188:drug therapy / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology
D007369:Interferon Inducers; D012333:RNA, Messenger; D011994:Recombinant Proteins; D018664:Interleukin-12; D007371:Interferon-gamma