Automated sample preparation for the TRUGENE HIV-1 genotyping kit using the MagNA pure LC instrument.
Use of the MagNA Pure LC Total Nucleic Acid Isolation Kit-Large Volume in conjunction with the TRUGENE HIV-1 Genotyping Kit yielded consistent, interpretable sequence data from 1-mL plasma preparations containing HIV-1 RNA concentrations of > or =400 copies/mL. This finding was confirmed in 18 of 24 low-titer clinical specimens.
Germer
Jeffrey J
JJ
Division of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota, USA.
Vandenameele
Jill N
JN
Mitchell
P Shawn
PS
Harmsen
W Scott
WS
Yao
Joseph D C
JD
D001331:Automation; D005838:Genotype; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D012367:RNA, Viral / Q000032:analysis; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012494:Sampling Studies; D012680:Sensitivity and Specificity
D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic