Synthesis of novel fluorescent-labelled dinucleoside polyphosphates.
A novel tandem synthetic-biosynthetic procedure is described for the synthesis of four new fluorescent dinucleoside polyphosphates: mant-Ap4A, mant-AppCH2ppA, TNP-Ap4A and TNP-AppCH2ppA. These compounds are expected to supplement the existing etheno (epsilon) and 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) labelled derivatives, being the fluorescent probes of choice to investigate polyphosphate/enzyme binding behaviour.
Wright
Michael
M
Imperial College Genetic Therapies Centre, Department of Chemistry, Flowers Building, Armstrong Road, Imperial College London, London SW7 2AZ, UK.
Miller
Andrew D
AD
D001896:Boron Compounds; D015226:Dinucleoside Phosphates / Q000138:chemical synthesis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D013057:Spectrum Analysis; D014301:Trinitrobenzenes; D062367:ortho-Aminobenzoates
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D015226:Dinucleoside Phosphates; D005456:Fluorescent Dyes; D014301:Trinitrobenzenes; D062367:ortho-Aminobenzoates