Determination of HIV type 1 CRF01_AE gag p17 and env-V3 consensus sequences for HIV/AIDS vaccine design.
A molecular epidemiological study of the gag p17 and env-V3 regions on HIV-infected drug users and blood donors was carried out in northern Thailand from 1998 through 2002 to determine the predominant subtype and consensus sequence (CS) for circulating HIV-1 strains. CRF01_AE was concluded to be a predominant strain and the nucleotide CSs in gag p17 and env-V3 showed only 1.26% and no difference from CS in the Los Alamos database, respectively. Our env-V3 CS was identical to the previously published CSs, suggesting that the CS was very conserved from 1990 through 2002 in Thailand. Gag p17 and env-V3 nucleotide sequences of seroconvertors in our subjects were quite similar to the CS and conserved for at least 9 and 6 years postinfection, respectively. These results suggest that the CS approach to the HIV-1 antigen design could overcome HIV diversity and help us develop an effective HIV/AIDS vaccine.
Hamano
Takaichi
T
JST AIDS Vaccine Project, c/o National Institute of Health, Ministry of Public Health, Nonthaburi 11000, Thailand. taka0627@yahoo.co.jp
Sawanpanyalert
Pathom
P
Yanai
Hideki
H
Piyaworawong
Surachai
S
Hara
Takashi
T
Sapsutthipas
Sompong
S
Phromjai
Jurairat
J
Yamazaki
Shudo
S
Yamamoto
Naoki
N
Warachit
Paijit
P
Honda
Mitsuo
M
Matsuo
Kazuhiro
K
D016915:AIDS Vaccines; D001782:Blood Donors; D016384:Consensus Sequence; D015195:Drug Design; D015683:Gene Products, gag / Q000235:genetics*; D015488:HIV Antigens / Q000235:genetics*; D015699:HIV Envelope Protein gp120 / Q000235:genetics*; D015658:HIV Infections / Q000453:epidemiology / Q000821:virology; D015497:HIV-1 / Q000145:classification / Q000235:genetics; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics*; D017422:Sequence Analysis, DNA; D015819:Substance Abuse, Intravenous / Q000150:complications; D013785:Thailand / Q000453:epidemiology; D014764:Viral Proteins / Q000235:genetics*; D054301:gag Gene Products, Human Immunodeficiency Virus
D016915:AIDS Vaccines; D015683:Gene Products, gag; D015488:HIV Antigens; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1