title: Rrs1p, a ribosomal protein L11-binding protein, is required for nuclear export of the 60S pre-ribosomal subunit in Saccharomyces cerevisiae.
abstract: Rrs1p is a ribosomal protein L11-binding protein in Saccharomyces cerevisiae. We have obtained temperature-sensitive rrs1 mutants by random PCR mutagenesis. [(3)H]Methionine pulse-chase analysis reveals that the rrs1 mutations cause a defect in maturation of 25S rRNA. Ribosomal protein L25-enhanced green fluorescent protein, a reporter of the 60S ribosomal subunit, concentrates in the nucleus with enrichment in the nucleolus when the rrs1 mutants are shifted to the restrictive temperature. These results suggest that Rrs1p stays on the pre-60S particle from the early stage to very late stage of the large-subunit maturation and is required for export of 60S subunits from the nucleolus to the cytoplasm.
authors.affiliation: Department of Bioresource Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Kagamiyama 1-3-1, Higashi-Hiroshima 739-8530, Japan.
authors.forename: Keita
authors.initials: K
authors.lastname: Miyoshi
authors.forename: Chiharu
authors.initials: C
authors.lastname: Shirai
authors.forename: Chihiro
authors.initials: C
authors.lastname: Horigome
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Takenami
authors.forename: Junko
authors.initials: J
authors.lastname: Kawasaki
authors.forename: Keiko
authors.initials: K
authors.lastname: Mizuta
mesh_terms: D021581:Active Transport, Cell Nucleus; D000483:Alleles; D000595:Amino Acid Sequence; D015153:Blotting, Western; D002352:Carrier Proteins / Q000737:chemistry* / Q000502:physiology*; D002466:Cell Nucleolus / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009154:Mutation; D009687:Nuclear Proteins; D010957:Plasmids / Q000378:metabolism; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000737:chemistry; D012269:Ribosomal Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D002352:Carrier Proteins; D008164:Luminescent Proteins; D009687:Nuclear Proteins; D012335:RNA, Ribosomal; C485770:RRS1 protein, S cerevisiae; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; C027624:ribosomal protein L11; C065039:RNA, ribosomal, 25S; D049452:Green Fluorescent Proteins; C083550:60S ribosomal protein kinase; D017346:Protein Serine-Threonine Kinases