[Effects of varglaucocalyx on c-fos gene expression during global myocardial ischemia-reperfusion in rat].
OBJECTIVE
To determine the effects of Varglaucocalyx on c-fos gene expression during global myocardial ischemia-reperfusion.


METHODS
Forty Wistar rats were divided into 5 groups: group N as control; group CN as ischemia-reperfusion control and group XH, XM and XL treated with Varglaucocalyx 5%, 1%, 0.5% respectively prior to ischemia-reperfusion. The isolated rat hearts were perfused in condition of constant temperature and pressure, and then the left ventricular myocardiums were extracted for use. The expression of c-fos protein was detected by immunochemical method. The expression of c-fos protein were quantified by using computer image analysis system.


RESULTS
Compared with the values of group N, protein expressions relative area of c-fos gene (PERA) were increased significantly in group CN, XH, XM, XL(P < 0.01), but decreased significantly in group XH, XM, XL, compared with those of group CN (P < 0.05). The PERA of c-fos gene in group XM, XL were significantly lower than in group XH (P < 0.01), and the PERA of c-fos gene in group XM were lower than in group XL(P < 0.05).


CONCLUSIONS
Varglaucocalyx can effectively depress the expression of c-fos gene in myocardium which may account for its protection against myocardial ischemia-reperfusion injury, and the middle and the low concentrations of Varglaucocalyx are more effective than the high concentrations.
Liu
Lan-di
LD
No. 202 Hospital Of PLA, Shenyang 110003, Liaoning, China.
Ye
Li-ka
LK
Pan
Dong-jun
DJ
Jiang
Yan-duo
YD
Zhang
Tie-zheng
TZ
Yang
Hui
H
D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D016762:Genes, fos; D037602:Isodon / Q000737:chemistry; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000209:etiology / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D002316:Cardiotonic Agents; D004365:Drugs, Chinese Herbal; D016760:Proto-Oncogene Proteins c-fos