Role of nucleostemin in growth regulation of gastric cancer, liver cancer and other malignancies.
OBJECTIVE
To examine the role of nucleostemin in the growth regulation of gastric cancer, liver cancer and other cancers.


METHODS
RT-PCR was used to clone the fragment of nucleostemin cDNA from HEK 293 cells. Eighteen kinds of malignant tumor tissues including gastric adenocarcinoma and liver cancer tissues, 3 kinds of benign tumor tissues, 3 kinds of benign hyperplastic tissues and normal tissues were employed to examine nucleostemin gene expression by RT-PCR, Slot blot, Northern blot and in situ hybridization.


RESULTS
We successfully cloned a 570 bp fragment of nucleostemin-cDNA from HEK-293 cells. All detected malignant tumor tissues, benign tumor tissues, and benign hyperplastic tissues had high levels of nucleostemin expression. Nucleostemin was also expressed in human placenta tissue at a high level. In terminally differentiated normal human adult kidney and mammary gland tissues, no nucleostemin expression could be detected.


CONCLUSIONS
Nucleostemin can help regulate the proliferation of both cancer cells and stem cells. It might play an important role in the growth regulation of gastric cancer, liver cancer and other cancers.
Liu
Si-Jin
SJ
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China.
Cai
Zi-Wei
ZW
Liu
Ya-Jun
YJ
Dong
Mei-Yu
MY
Sun
Li-Qiu
LQ
Hu
Guo-Fa
GF
Wei
Ying-Yun
YY
Lao
Wei-De
WD
D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D005260:Female; D019204:GTP-Binding Proteins; D005786:Gene Expression Regulation; D006801:Humans; D017403:In Situ Hybridization; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution
D002352:Carrier Proteins; C469949:GNL3 protein, human; D009687:Nuclear Proteins; D019204:GTP-Binding Proteins