[Effect of ouabain, iodoacetamide, and pH on the human erythrocyte hemolysis induced by calcium ions in the presence of ionophore A23187].
The effect of inhibitors of Na+, K(+)-ATPase (ouabain) and glycolysis (iodacetamide) as well as pH on calcium ion-induced erythrocyte hemolysis in the presence of ionophore A23187 is first described. Hemoglobin release decreases under the influence of ouabain, iodacetamide, and low pH, which is commonly observed at low temperature and in the samples studied in spring and summer. Active hemoglobin release through defects of the erythrocyte membrane under the influence of the transmembrane electrical potential was proposed to mediate hemolysis.
Marachev
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AG
Institute of Biological Problems of the North, Far East Division, Russian Academy of Sciences, ul. Portovaya 18, 685000 Russia.
Lapinskiĭ
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AG
D000293:Adolescent; D000328:Adult; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects* / Q000473:pathology; D006454:Hemoglobins / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007460:Iodoacetamide / Q000494:pharmacology*; D007476:Ionophores / Q000494:pharmacology*; D007477:Ions; D008297:Male; D010042:Ouabain / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors; D013696:Temperature
D004791:Enzyme Inhibitors; D006454:Hemoglobins; D007476:Ionophores; D007477:Ions; D000001:Calcimycin; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase; D002118:Calcium; D007460:Iodoacetamide