Apoptosis-like cell death of human breast cancer cell line MCF-7 induced by buprenorphine hydrochloride.
The analgesic buprenorphine hydrochloride (Bph) induced apoptosis-like cell death in the caspase-3-deficient human breast cancer cell line, MCF-7. This apoptosis-like cell death activated key molecules in the mitochondrial apoptotic pathway: cytochrome c, caspase-9, caspase-7, and caspase-6. Bph caused the release of fluorescent protein from the mitochondria of MCF-7 cells transfected with the pDsRed2-Mito-vector in a time-dependent manner, suggesting disruption of the mitochondrial membrane. Zn(2+) as high as 2 mM did not inhibit the DNase that took part in this apoptosis. Thus, this unidentified DNase might resemble other DNases involved in apoptosis-like cell death whose activity is not inhibited by zinc ion.
Kugawa
Fumihiko
F
Department of Biological Pharmaceutical Sciences, College of Pharmacy, Nihon University, 7-7-1 Narashino-dai, Funabashi, Chiba 274-8555, Japan. jfkugawa@pha.nihon-u.ac.jp
Matsumoto
Kazuhiko
K
Aoki
Masatada
M
D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000473:pathology*; D002047:Buprenorphine / Q000494:pharmacology*; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival; D045304:Cytochromes c / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D003851:Deoxyribonucleases / Q000378:metabolism; D004789:Enzyme Activation / Q000502:physiology; D005260:Female; D006801:Humans; D008928:Mitochondria / Q000187:drug effects; D009292:Narcotic Antagonists / Q000494:pharmacology*; D014162:Transfection; D015032:Zinc / Q000494:pharmacology
D009292:Narcotic Antagonists; D002047:Buprenorphine; D045304:Cytochromes c; D003851:Deoxyribonucleases; D020169:Caspases; D015032:Zinc