title: Genome sequence of the lignocellulose degrading fungus Phanerochaete chrysosporium strain RP78.
abstract: White rot fungi efficiently degrade lignin, a complex aromatic polymer in wood that is among the most abundant natural materials on earth. These fungi use extracellular oxidative enzymes that are also able to transform related aromatic compounds found in explosive contaminants, pesticides and toxic waste. We have sequenced the 30-million base-pair genome of Phanerochaete chrysosporium strain RP78 using a whole genome shotgun approach. The P. chrysosporium genome reveals an impressive array of genes encoding secreted oxidases, peroxidases and hydrolytic enzymes that cooperate in wood decay. Analysis of the genome data will enhance our understanding of lignocellulose degradation, a pivotal process in the global carbon cycle, and provide a framework for further development of bioprocesses for biomass utilization, organopollutant degradation and fiber bleaching. This genome provides a high quality draft sequence of a basidiomycete, a major fungal phylum that includes important plant and animal pathogens.
authors.affiliation: US DoE Joint Genome Institute, Walnut Creek, California 94598, USA.
authors.forename: Diego
authors.initials: D
authors.lastname: Martinez
authors.forename: Luis F
authors.initials: LF
authors.lastname: Larrondo
authors.forename: Nik
authors.initials: N
authors.lastname: Putnam
authors.forename: Maarten D Sollewijn
authors.initials: MD
authors.lastname: Gelpke
authors.forename: Katherine
authors.initials: K
authors.lastname: Huang
authors.forename: Jarrod
authors.initials: J
authors.lastname: Chapman
authors.forename: Kevin G
authors.initials: KG
authors.lastname: Helfenbein
authors.forename: Preethi
authors.initials: P
authors.lastname: Ramaiya
authors.forename: J Chris
authors.initials: JC
authors.lastname: Detter
authors.forename: Frank
authors.initials: F
authors.lastname: Larimer
authors.forename: Pedro M
authors.initials: PM
authors.lastname: Coutinho
authors.forename: Bernard
authors.initials: B
authors.lastname: Henrissat
authors.forename: Randy
authors.initials: R
authors.lastname: Berka
authors.forename: Dan
authors.initials: D
authors.lastname: Cullen
authors.forename: Daniel
authors.initials: D
authors.lastname: Rokhsar
mesh_terms: D001482:Base Composition / Q000235:genetics; D001673:Biodegradation, Environmental; D002482:Cellulose / Q000378:metabolism*; D002965:Classification; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D005091:Exons / Q000235:genetics; D005656:Fungal Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D015723:Gene Library; D005800:Genes, Fungal / Q000235:genetics; D016681:Genome, Fungal; D023281:Genomics; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism; D007438:Introns / Q000235:genetics; D042845:Laccase / Q000235:genetics / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism; D020075:Phanerochaete / Q000235:genetics* / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D018626:Retroelements / Q000235:genetics; D017422:Sequence Analysis, DNA; D019895:Transposases / Q000235:genetics
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D011134:Polysaccharides; D018626:Retroelements; C036909:lignocellulose; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D042845:Laccase; D010544:Peroxidases; D016695:Glycosyltransferases; D019895:Transposases; D006026:Glycoside Hydrolases