title: The control of Staphylococcus epidermidis biofilm formation and in vivo infection rates by covalently bound furanones.
abstract: In order to overcome the continuing infection rate associated with biomaterials, the use of covalently bound furanones as an antibiofilm coating for biomaterials has been investigated. Furanones have previously been shown to inhibit growth of Gram-positive and Gram-negative bacteria. The aim of these studies were to covalently bind furanones to polymers and to test their efficacy for inhibiting biofilm formation of Staphylococcus epidermidis and in vivo infection rate. Two methods of covalent attachment of furanones were used. The first, a co-polymerisation with a styrene polymer, and second, a plasma-1-ethyl-3-(dimethylaminopropyl) carbodiimide (EDC) reaction to produce furanone-coated catheters. Biofilm formation by S. epidermidis in vitro was inhibited by 89% for polystryene-furanone disks and by 78% by furanone-coated catheters (p<0.01). In an in vivo sheep model we found furanones were effective at controlling infection for up to 65 days. Furanones have potential to be used as a coating for biomaterials to control infection caused by S. epidermidis.
authors.affiliation: Cooperative Research Centre for Eye Research and Technology, Vision CRC, The University of New South Wales, Sydney, NSW 2052, Australia e.hume@visioncrc.org
authors.forename: E B H
authors.initials: EB
authors.lastname: Hume
authors.forename: J
authors.initials: J
authors.lastname: Baveja
authors.forename: B
authors.initials: B
authors.lastname: Muir
authors.forename: T L
authors.initials: TL
authors.lastname: Schubert
authors.forename: N
authors.initials: N
authors.lastname: Kumar
authors.forename: S
authors.initials: S
authors.lastname: Kjelleberg
authors.forename: H J
authors.initials: HJ
authors.lastname: Griesser
authors.forename: H
authors.initials: H
authors.lastname: Thissen
authors.forename: R
authors.initials: R
authors.lastname: Read
authors.forename: L A
authors.initials: LA
authors.lastname: Poole-Warren
authors.forename: K
authors.initials: K
authors.lastname: Schindhelm
authors.forename: M D P
authors.initials: MD
authors.lastname: Willcox
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001672:Biocompatible Materials; D018441:Biofilms; D001704:Biopolymers; D002404:Catheterization; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry*; D005663:Furans / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D011485:Protein Binding; D012756:Sheep; D013203:Staphylococcal Infections / Q000378:metabolism*; D013212:Staphylococcus epidermidis / Q000378:metabolism*; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D001704:Biopolymers; D005663:Furans; D011108:Polymers; D011137:Polystyrenes; D005022:Ethyldimethylaminopropyl Carbodiimide; C039281:furan