[Use of genetically modified embryonic stem cells for creating transgenic animals].
Targeted genetic modification of embryonic stem cells (ESC) was used to obtain nondifferentiated cell clones containing the foreign genetic material in the genome. It was demonstrated that transgenic animals may be obtained by ESC injection in preimplantation embryos and subsequent transplantation of the embryos into a recipient female. Using this method, we constructed chimeric animals with a modified genome.
Pryzhkova
M V
MV
Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
Zakeeva
I R
IR
Kibardin
A V
AV
Georgiev
G P
GP
Kiselev
S L
SL
D000818:Animals; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D013234:Stem Cells / Q000166:cytology*