Cdc14 and condensin control the dissolution of cohesin-independent chromosome linkages at repeated DNA.
Chromosome segregation is triggered by the cleavage of cohesins by separase. Here we show that in budding yeast separation of the ribosomal DNA (rDNA) and telomeres also requires Cdc14, a protein phosphatase known for its role in mitotic exit. Cdc14 shares this role with the FEAR network, which activates Cdc14 during early anaphase, but not the mitotic exit network, which promotes Cdc14 activity during late anaphase. We further show that CDC14 is necessary and sufficient to promote condensin enrichment at the rDNA locus and to trigger rDNA segregation in a condensin-dependent manner. We propose that Cdc14 released by the FEAR network mediates the partitioning of rDNA by facilitating the localization of condensin thereto. This dual role of the FEAR network in initiating mitotic exit and promoting chromosome segregation ensures that exit from mitosis is coupled to the completion of chromosome segregation.
D'Amours
Damien
D
Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, E17-233, 40 Ames Street, Cambridge, MA 02139, USA.
Stegmeier
Frank
F
Amon
Angelika
A
D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D002466:Cell Nucleolus / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D002868:Chromosomal Proteins, Non-Histone; D020090:Chromosome Segregation / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins; D008938:Mitosis / Q000235:genetics; D046912:Multiprotein Complexes; D009154:Mutation / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics; D017027:Protein Tyrosine Phosphatases / Q000235:genetics* / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D025842:SUMO-1 Protein / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D016615:Telomere / Q000235:genetics; D000097722:Cohesins
D000251:Adenosine Triphosphatases; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D004275:DNA, Ribosomal; D004268:DNA-Binding Proteins; D010450:Endopeptidases; D005656:Fungal Proteins; D046912:Multiprotein Complexes; D009687:Nuclear Proteins; D017027:Protein Tyrosine Phosphatases; D025842:SUMO-1 Protein; D029701:Saccharomyces cerevisiae Proteins; D000097722:Cohesins; C074860:CDC14 protein, S cerevisiae; C108312:condensin complexes; C405949:ULP2 protein, S cerevisiae