[Study on the solubility changes of Rhizoma Coptidis' main chemical constituents influenced by the proportion of Rhizoma Coptidis and Fructus Evodiae].
OBJECTIVE
To investigate the relationship between the solubility of Rhizoma Coptidis' main chemical constituents and the proportion of Rhizoma Coptidis and Fructus Evodiae.


METHODS
9 samples were selected in which the proportion of Rhizoma Coptidis and Fructus Evodiae were 1:0, 6:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:6 and 0:1, respectively. The samples' water-extracts were analyzed by RP-HPLC. Then solubility changes of eight chemical constituents were calculated, and the regression equations were established.


RESULTS
Among the eight chemical constituents, one was independent on the change of proportion of Rhizoma Coptidis and Fructus Evodiae, six having linear relationship. A new constituent was detected, and there was nonlinear relationship between its quantity and the proportion of the couple.


CONCLUSIONS
The solubility of Rhizoma Coptidis' main chemical constituents declines almost in the same scale when Rhizoma Coptidis and Fructus Evodiae are used as a couple, and a new constituent has been detected which can't be found in the mono-extraction.
Peng
Ming-xing
MX
Department of Chinese Medine Science and Engineering, Zhejiang University, Hangzhou 310027, Zhejiang, China.
Wu
Yong-jiang
YJ
Cheng
Yi-yu
YY
D001599:Berberine / Q000031:analogs & derivatives* / Q000032:analysis; D001600:Berberine Alkaloids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D039323:Coptis / Q000737:chemistry*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D032086:Evodia / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012995:Solubility
D001600:Berberine Alkaloids; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C055785:jatrorrhizine; C034384:coptisine; D001599:Berberine; C005413:palmatine