Accurate assignment of ethanol origin in postmortem urine: liquid chromatographic-mass spectrometric determination of serotonin metabolites.
Toxicological examination of fatal aviation accident victims routinely includes analysis of ethanol levels. However, distinguishing between antemortem ingestion and postmortem microbial formation complicates all positive ethanol results. Development of a single analytical approach to determine concentrations of 5-hydroxytryptophol (5-HTOL) and 5-hydroxyindole-3-acetic acid (5-HIAA), two well-known metabolites of serotonin, has provided a convenient, rapid and reliable solution to this problem. Antemortem ethanol leads to an elevation in the 5-HTOL/5-HIAA ratio for 11-19 h after acute ingestion. The liquid-liquid extracts of postmortem urine samples were subjected to liquid chromatography-mass spectrometry (LC-MS) for the simultaneous quantitation of these two analytes, yielding detection limits of 0.1 ng/ml for each. Examination of the 5-HTOL/5-HIAA ratio was undertaken for 44 urine samples known to be antemortem ethanol-positive or antemortem ethanol-negative. Recent ethanol ingestion was conveniently and accurately separated using a 5-HTOL/5-HIAA ratio of 15 pmol/nmol, a value previously suggested using human volunteers. All 21 ethanol-negative postmortem samples were below this cutoff, while all 23 ethanol-positive postmortem samples were above this cutoff. Thus, we recommend the employment of this cutoff value, established using this straightforward LC-MS procedure, to confirm or deny recent antemortem ethanol ingestion in postmortem urine samples.
Johnson
R D
RD
Federal Aviation Administration, Civil Aerospace Medical Institute, AAM-610, Oklahoma City, OK 73169-6901, USA. r.d.johnson@faa.gov
Lewis
R J
RJ
Canfield
D V
DV
Blank
C L
CL
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000431:Ethanol / Q000652:urine*; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000652:urine*; D006917:Hydroxytryptophol / Q000652:urine*; D013058:Mass Spectrometry / Q000379:methods*; D011180:Postmortem Changes; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D006917:Hydroxytryptophol; D000431:Ethanol; D006897:Hydroxyindoleacetic Acid