Electrochemical determination of lead and glutathione in a plant cell culture.
In this work we established differential pulse anodic stripping voltammetry (DPASV) as the tool for analysis of lead in the plant cell culture. For the cultivation procedure, lead in Pb(II)-ethylenediaminetetraacetic acid (Pb-EDTA) chelate has been used. The detection limit of lead was found at 500 pM in phosphate buffer (pH 5.5), and 100 nM in prepared cells intracellular extract (20 pg Pb(II)/mg cells). For determination of cysteine-rich peptides, voltammetry in differential mode (DPV) in cobalt(III)-containing ammonia buffer (Brdicka reaction) was used. In this short communication, we present suitable voltammetric techniques for the physiological study of lead and thiols in plant cell culture.
Vacek
Jan
J
Department of Botany and Plant Physiology, Mendel University of Agriculture and Forestry, Zemedelska 1, 613 00 Brno, Czech Republic.
Petrek
Jirí
J
Kizek
René
R
Havel
Ladislav
L
Klejdus
Borivoj
B
Trnková
Libuse
L
Jelen
Frantisek
F
D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D004563:Electrochemistry / Q000379:methods*; D005978:Glutathione / Q000032:analysis* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007854:Lead / Q000032:analysis* / Q000378:metabolism; D028222:Picea / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000032:analysis* / Q000378:metabolism
D013438:Sulfhydryl Compounds; D007854:Lead; D005978:Glutathione