Sensitization of recombinant vanilloid receptor-1 by various neurotrophic factors.
The vanilloid receptor (VR1) is a central integrator molecule of nociceptive stimuli. In this study, we have measured the effects of various neurotrophins (nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3, and -4) on recombinant rat VR1-mediated intracellular calcium rise in response to capsaicin in VR1/C6 cells. Our results clearly show that all neurotrophins sensitize the VR1 to capsaicin. Furthermore, using K252a, an inhibitor of tyrosine kinases, we present that actions of neurotrophins are mediated by the trk (A, B, C) receptors expressed in these cells. These data argue for the putative roles of neurotrophins in inducing inflammatory (thermal) hyperalgesia via VR1.
Lázár
József
J
Department of Physiology and Cell Physiology Research Group of the Hungarian Academy of Sciences, H-4012 Debrecen, Nagyerdei krt. 98. PO Box 22, Hungary.
Szabó
Tamás
T
Marincsák
Rita
R
Kovács
László
L
Blumberg
Peter M
PM
Bíró
Tamás
T
D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism*; D002211:Capsaicin / Q000378:metabolism*; D006930:Hyperalgesia / Q000139:chemically induced; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009619:Nociceptors / Q000187:drug effects*; D051381:Rats; D011955:Receptors, Drug / Q000187:drug effects*; D011994:Recombinant Proteins / Q000187:drug effects; D050916:TRPV Cation Channels; D014162:Transfection; D014407:Tumor Cells, Cultured
D009414:Nerve Growth Factors; D011955:Receptors, Drug; D011994:Recombinant Proteins; D050916:TRPV Cation Channels; C494484:TRPV1 receptor; D002211:Capsaicin; D002118:Calcium