Presence of bacterial phage-like DNA sequences in commercial Taq DNA polymerase reagents.
Many studies have reported the presence of bacterial DNA contamination in commercial Taq DNA polymerase reagents. This is the first report of the presence of phage-like DNA sequences in certain commercial Taq DNA polymerase reagents. Precautions are needed when using amplification reagents with exogenous DNAs.
Newsome
Tamara
T
Department of Infectious and Parasitic Disease Pathology, American Registry of Pathology, Washington, DC, USA.
Li
Bing-Jie
BJ
Zou
Nianxiang
N
Lo
Shyh-Ching
SC
D001435:Bacteriophages / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification*; D004340:Drug Contamination; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D019914:Taq Polymerase
D017931:DNA Primers; D004279:DNA, Viral; D007202:Indicators and Reagents; D019914:Taq Polymerase