[Comparative studies on proteins of cytoplasmic male-sterile wheat and its maintainer by 2D-PAGE in Triticum aestivum].
By using 2D-PAGE techniques, protein compositions have been studied at different developmental stage leaf (seedling, tillering, shooting and booting stages) and pollen mother cells (PMC) (meiotic, 1-nucleus and 2-3 nuclei stages) in male sterile wheat and its maintainer. The results indicated that a specific protein (33KD/pI6.3) identified at the shooting and booting stage leaves in male-sterile wheat, but this protein species has not been identified in the male fertile wheat. Four protein species, 53KD/PI5.5, 50KD/PI5.7, 48KD/PI5.6, 20KD/PI7.5 were detected at the 2-3 nuclei stages, which was thought key stage of pollen fertility lost in male-sterile wheat, and these protein species were not be seen in male-fertile wheat. According above experiment results, five protein species which detected in leaf and PMC might participate in fertility regulation and related to the male-sterility of male-sterile wheat.
Fan
Bao Li
BL
Department of Biology, Tianjin Normal University, Tianjin 300074.
Wang
Zhen Ying
ZY
Chen
Hong
H
Peng
Yong Kang
YK
D015180:Electrophoresis, Gel, Two-Dimensional; D005298:Fertility; D008540:Meiosis; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D011058:Pollen / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism; D014908:Triticum / Q000254:growth & development / Q000378:metabolism*
D010940:Plant Proteins