A new model of Hantaan virus persistence in mice: the balance between HTNV infection and CD8(+) T-cell responses.
We established a viral persistence model that involves the adoptive transfer of spleen cells from immunocompetent mice (H-2(d)) into Hantaan virus (HTNV)-infected severe combined immunodeficient (SCID, H-2(d)) mice. The infection is maintained despite the presence of neutralizing antibodies, without apparent signs of disease, and there is a correlation between HTNV persistence and the lack of HTNV-specific CD8(+) T cells. In addition, disseminated HTNV infection before the initiation of immune responses appears to be important for virus persistence. The suppression of HTNV-specific CD8(+) T cells in the present model appears to occur at the periphery. The present study also demonstrates that CD8(+) T cells contribute to the clearance of HTNV. Thus, it seems that HTNV-specific CD8(+) T cells play a key role in HTNV persistence in mice. This model of viral persistence is useful for studies of immune responses and immunocytotherapy against viral infection.
Araki
Koichi
K
Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Yoshimatsu
Kumiko
K
Lee
Byoung-Hee
BH
Kariwa
Hiroaki
H
Takashima
Ikuo
I
Arikawa
Jiro
J
D019264:Adoptive Transfer; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D036022:Capsid Proteins / Q000378:metabolism; D002908:Chronic Disease; D003602:Cytotoxicity, Immunologic; D004195:Disease Models, Animal; D018063:Hantaan virus / Q000276:immunology / Q000472:pathogenicity*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000276:immunology* / Q000503:physiopathology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D014758:Viral Core Proteins / Q000378:metabolism
D036022:Capsid Proteins; D014758:Viral Core Proteins; C111782:nucleocapsid protein, Hantaan virus