title: Alterations in expression of monocyte chemotactic protein-1 in the simian immunodeficiency virus model of disseminated Mycobacterium avium complex.
abstract: Mycobacterium avium complex (MAC) infection is the most common disseminated bacterial infection in untreated patients with acquired immunodeficiency syndrome (AIDS). We investigated the potential role of monocyte chemotactic protein-1 (MCP-1) in the pathogenesis of disseminated MAC, using the simian immunodeficiency virus (SIV)/macaque model of AIDS. Macaques were inoculated with SIV, followed by challenge with a pathogenic AIDS isolate of M. avium 14 days later. After challenge with M. avium, marked increases in serum MCP-1 levels were detected in SIV-infected macaques, a finding that was duplicated in coinoculated bronchoalveolar macrophages. MCP-1 levels were significantly higher in SIV-infected macaques than in non-SIV-infected controls (327.1 vs. 151.5 pg/mL, respectively; P=.04), suggesting that up-regulation of MCP-1 contributes to the development of progressive mycobacterial disease. Similarly, morphometric analysis revealed increased expression of MCP-1 in hepatic microgranulomas from SIV-infected macaques. We conclude that the pronounced increases in MCP-1 levels demonstrated in tissue and serum samples after M. avium inoculation may play a role in the development of disseminated mycobacterial disease.
authors.affiliation: Department of Pathobiology and Veterinary Sciences, University of Connecticut, Storrs, Connecticut, USA.
authors.forename: Elizabeth E
authors.initials: EE
authors.lastname: Hendricks
authors.forename: Kuei-Chin
authors.initials: KC
authors.lastname: Lin
authors.forename: Karen
authors.initials: K
authors.lastname: Boisvert
authors.forename: Douglas
authors.initials: D
authors.lastname: Pauley
authors.forename: Keith G
authors.initials: KG
authors.lastname: Mansfield
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D018932:Chemokine CCL2 / Q000378:metabolism*; D004195:Disease Models, Animal; D006801:Humans; D008253:Macaca mulatta; D008264:Macrophages / Q000382:microbiology / Q000821:virology; D015269:Mycobacterium avium Complex / Q000472:pathogenicity*; D015270:Mycobacterium avium-intracellulare Infection / Q000382:microbiology / Q000503:physiopathology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000150:complications*; D015302:Simian Immunodeficiency Virus / Q000472:pathogenicity*
chemical_list: D018932:Chemokine CCL2