title: Dynamic populations of dendritic cell-specific ICAM-3 grabbing nonintegrin-positive immature dendritic cells and liver/lymph node-specific ICAM-3 grabbing nonintegrin-positive endothelial cells in the outer zones of the paracortex of human lymph nodes.
abstract: In the paracortex of lymph nodes, cellular immune responses are generated against antigens captured in peripheral tissues by dendritic cells (DCs). DC-SIGN (dendritic cell-specific ICAM-3 grabbing nonintegrin), a C-type lectin exclusively expressed by DCs, functions as an antigen receptor as well as an adhesion receptor. A functional homologue of DC-SIGN, L-SIGN (liver/lymph node-SIGN, also called DC-SIGN-related), is expressed by liver sinus endothelial cells. In lymph nodes, both DC-SIGN and L-SIGN are expressed. In this study, we analyzed the distribution of these two SIGN molecules in detail in both normal and immunoreactive lymph nodes. DC-SIGN is expressed by mature DCs in paracortical areas and in addition by DCs with an immature phenotype in the outer zones of the paracortex. L-SIGN expression was also detected in the outer zones on sinus endothelial cells characterized by their expression of the lymphatic endothelial markers LYVE-1 and CLEVER-1. During both cellular and humoral immune responses changes in the amount of DC-SIGN+ immature and mature DCs and L-SIGN+ endothelial cells were observed, indicating that the influx or proliferation of these cells is dynamically regulated.
authors.affiliation: Department of Molecular Cell Biology and Immunology, Vrije Universiteit Medical Center, Amsterdam, The Netherlands.
authors.forename: Anneke
authors.initials: A
authors.lastname: Engering
authors.forename: Sandra J
authors.initials: SJ
authors.lastname: van Vliet
authors.forename: Konnie
authors.initials: K
authors.lastname: Hebeda
authors.forename: David G
authors.initials: DG
authors.lastname: Jackson
authors.forename: Remko
authors.initials: R
authors.lastname: Prevo
authors.forename: Satwinder K
authors.initials: SK
authors.lastname: Singh
authors.forename: Teunis B H
authors.initials: TB
authors.lastname: Geijtenbeek
authors.forename: Han
authors.initials: H
authors.lastname: van Krieken
authors.forename: Yvette
authors.initials: Y
authors.lastname: van Kooyk
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002455:Cell Division; D003713:Dendritic Cells / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D016023:Integrins / Q000378:metabolism*; D020014:K562 Cells; D037102:Lectins / Q000737:chemistry; D037181:Lectins, C-Type / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism*; D008207:Lymphatic Metastasis; D010641:Phenotype; D011956:Receptors, Cell Surface / Q000378:metabolism*; D014018:Tissue Distribution; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D016023:Integrins; D037102:Lectins; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C433029:CLEC4M protein, human; C483616:ICAM3 protein, human