title: 3-Amino-1,4-dimethyl-5H-pyrido[4,3-b]indole induces apoptosis and necrosis with activation of different caspases in rat splenocytes.
abstract: A dietary carcinogen, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) at 20 microM activates caspase-3-like proteases as an apoptotic marker in rat splenocytes. The present study demonstrated 100 microM Trp-P-1 induced necrosis with activation of caspase-3-like proteases. The activation in necrosis and apoptosis resulted from the activation of caspase-9 and caspase-8, respectively. Thus, Trp-P-1 induces apoptosis and necrosis with the activation of different caspases.
authors.affiliation: Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, USA.
authors.forename: Takashi
authors.initials: T
authors.lastname: Hashimoto
authors.forename: Takashi
authors.initials: T
authors.lastname: Sano
authors.forename: Wakana
authors.initials: W
authors.lastname: Ito
authors.forename: Kazuki
authors.initials: K
authors.lastname: Kanazawa
authors.forename: Gen-ichi
authors.initials: G
authors.lastname: Danno
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ashida
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002243:Carbolines / Q000494:pharmacology*; D020169:Caspases / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D009336:Necrosis; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013154:Spleen / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism
chemical_list: D002243:Carbolines; D017382:Reactive Oxygen Species; D020169:Caspases; C019132:3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole