[Multiple forms of the proteasomes and some approaches to their separation].
A technique for proteasome isolation from rat liver was developed; it allows isolation of 26S proteasome fraction free from 20S proteasome admixture. The technique includes consecutive stages of protein fractionation by different concentrations of ammonium sulfate, gel filtration on Sepharose 2B, and high-speed ultracentrifugation.
Abramova
E B
EB
Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 119991 Russia.
Astakhova
T M
TM
Erokhov
P A
PA
Sharova
N P
NP
D000645:Ammonium Sulfate / Q000737:chemistry; D000818:Animals; D001671:Biochemistry / Q000379:methods*; D002850:Chromatography, Gel / Q000379:methods; D009955:Ornithine Decarboxylase / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats; D014461:Ultracentrifugation / Q000379:methods
D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease; D009955:Ornithine Decarboxylase; D000645:Ammonium Sulfate