[Protective effect of ONO-1078, a leukotriene receptor antagonist, on focal cerebral ischemia induced by endothelin-1 in rats].
OBJECTIVE
To determine the protective effect of ONO-1078, a leukotriene receptor antagonist, on focal cerebral ischemia induced by endothelin-1 in rats.


METHODS
Slow microinjection of endothelin-1 (120 pmol in 6 microL, for > 6 min) into the region near the middle cerebral artery was used to induce focal cerebral ischemia. ONO-1078 (0.1 mg.kg-1) was i.p. injected 1 h before endothelin-1 injection. Neurological symptoms, brain edema, brain infarction size, and the survival neurons in cortex and striatum were observed 24 h after ischemia.


RESULTS
Intracerebral microinjection of endothelin-1 induced remarkable neurological symptoms, brain infarction, brain edema, and decrease of survival neurons in the cortex and striatum. In rats pretreated with ONO-1078, endothelin-1-induced brain edema and brain infarction size were decreased. The numbers of survival neurons in striatum and cortex were increased significantly. The neurological symptoms were improved but not significantly.


CONCLUSIONS
ONO-1078 possesses neuroprotective effect against cerebral ischemic injury induced by endothelin-1, therefore, leukotrienes may play a role in the injury of cerebral ischemia.
Zhang
Shi-hong
SH
Department of Pharmacology, Medical School, Zhejiang University, Hangzhou 310031, China.
Wei
Er-qing
EQ
Zhu
Chao-yang
CY
Chen
Zhong
Z
Zhang
Song-fa
SF
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001929:Brain Edema / Q000473:pathology; D002545:Brain Ischemia / Q000139:chemically induced / Q000473:pathology*; D002540:Cerebral Cortex / Q000473:pathology; D002544:Cerebral Infarction / Q000473:pathology; D002867:Chromones / Q000494:pharmacology*; D003342:Corpus Striatum / Q000473:pathology; D019332:Endothelin-1; D020024:Leukotriene Antagonists / Q000494:pharmacology*; D008297:Male; D009474:Neurons / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D002867:Chromones; D019332:Endothelin-1; D020024:Leukotriene Antagonists; D018696:Neuroprotective Agents; C047681:pranlukast