Lipoxygenase inhibitory activity of octyl gallate.
Octyl gallate inhibited soybean lipoxygenase-1 (EC 1.13.11.12, type I) with an IC(50) of 1.3 microM. The inhibition of the enzyme by octyl gallate is a slow and reversible reaction without residual activity. The inhibition kinetics analyzed by Lineweaver-Burk plots indicates that octyl gallate is a competitive inhibitor, and the inhibition constant, K(I), was obtained as 0.54 microM. One molecule of octyl gallate scavenged six molecules of 1,1-diphenyl-2-picrylhydrazyl and inhibited autoxidative lipid peroxidation. In addition, octyl gallate was effective in preventing lipid peroxidation.
Ha
Tae Joung
TJ
Department of Environmental Science, Policy and Management, University of California, Berkeley, California 94720-3112, USA.
Nihei
Ken-ichi
K
Kubo
Isao
I
D001667:Binding, Competitive; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects; D008084:Lipoxygenase / Q000378:metabolism; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology*; D013025:Glycine max / Q000201:enzymology*
D016859:Lipoxygenase Inhibitors; C016627:octyl gallate; D005707:Gallic Acid; D008084:Lipoxygenase