Effect of halothane on the release of [Ca2+]i in dorsal root ganglion neurons.
We investigated the effect of the volatile anaesthetic halothane on [Ca2+]i of dorsal root ganglion neurons. Halothane was able to increase [Ca2+]i in those neurons in a dose-dependent manner and independent of extracellular calcium. However, halothane action was inhibited by BAPTA-AM, suggesting the involvement of intracellular calcium stores. Dantrolene, an inhibitor of ryanodine-sensitive calcium stores had no effect while 2-APB, an inhibitor of IP3-sensitive calcium store reduced by 78% the halothane-evoked increase on [Ca2+]i. These data suggests that halothane increased [Ca2+]i of ganglion neurons through calcium release from IP3-sensitive calcium store. One possible consequence of the halothane action is to alter presynaptic activity and signaling pathways that influence neurotransmission.
Gomes
Dawidson A
DA
Departamento de Farmacologia, Fisiologia e Biofísica, UFMG, Belo Horizonte, Minas Gerais, Brazil.
Guatimosim
Cristina
C
Gomez
Renato Santiago
RS
Leite
Maria F
MF
Vieira
Luciene B
LB
Prado
Marco A
MA
Romano-Silva
Marco A
MA
Gomez
Marcus V
MV
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005727:Ganglia, Spinal / Q000187:drug effects* / Q000378:metabolism; D006221:Halothane / Q000494:pharmacology*; D007424:Intracellular Fluid / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism
D002118:Calcium; D006221:Halothane