Penta-acetyl geniposide-induced C6 glioma cell apoptosis was associated with the activation of protein kinase C-delta.
Herbal medicine has been utilized to treat a variety of diseases, including cancer. On the other hand, disturbance of apoptosis is often observed in cancer cells. It has been reported that protein kinase C (PKC) isoforms are involved in the signaling of apoptosis. In the present study, we investigate the antitumor effect and possible mechanism of a herbal-originated product, (Ac)(5)GP. We demonstrate that (Ac)(5)GP treatment results in DNA fragmentation of C6 glioma cells dose-dependently. Stimulated by (Ac)(5)GP, PKCdelta and PKCzeta were activated and translocated to the cell membrane fraction. Flow cytometry analysis showed that PKCdelta, but not PKCzeta inhibition blocks the (Ac)(5)GP-induced apoptosis by decreasing the cell population of sub G1 peak. However, the mRNA levels of PKCdelta and PKCzeta were not altered by (Ac)(5)GP-induced glioma cell apoptosis. These results suggested that the treatment of (Ac)(5)GP induces apoptosis of tumor cells through the activation but not the synthesis of PKCdelta.
Peng
Chiung-Huei
CH
Institute of Biochemistry, Chung Shan Medical University, No. 110, Section 2, Chien Kuo N. Road, Taichung 402, Taichung, Taiwan, ROC.
Tseng
Tsui-Hwa
TH
Liu
Jer-Yuh
JY
Hsieh
Yi-Hsien
YH
Huang
Chien-Ning
CN
Hsu
Shu-Ping
SP
Wang
Chau-Jong
CJ
D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D004789:Enzyme Activation; D032062:Gardenia / Q000737:chemistry; D005910:Glioma / Q000201:enzymology / Q000473:pathology*; D011493:Protein Kinase C / Q000378:metabolism*; D051745:Protein Kinase C-delta; D011714:Pyrans / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats
C469218:1-(2',3',4',6'-tetraacetylglucopyranosyloxyl)-1,4a,5,7a-tetrahydro-7-(acetomethyl)-cyclopentapyran-4-caroboxylic acid methyl ester; D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; D011714:Pyrans; C497260:Prkcd protein, rat; D011493:Protein Kinase C; D051745:Protein Kinase C-delta