title: [Study on preparation process and analytical methods of ESAC from Ganoderma lucidum].
abstract: OBJECTIVE
  To develop the procedure for separating the ethanol-soluble and acidic components (ESAC) from Ganoderma lucidum, and to establish a method for quantifying ESAC in G. lucidum.
  
  
  METHODS
  The ethanol extract of G. lucidum was extracted with a saturated NaHCO3 solution, acidified and re-extracted by chloroform to obtain ESAC. The quantitative analysis of ESAC was based on the characteristic color reaction between ESAC and H2SO4.
  
  
  RESULTS
  The optimal conditions for separating ESAC on a 10 g scale are as follows: ratio of material and ethanol (mL), 1:15; immersing time, 24 h; volume of saturated NaHCO3 and chloroform, 1300 mL; extract 3 times. The condition for measuring ESAC is as follows: sample weight, 1 g; solution volume, 1.5 mL; immuersing time, 0.5 h; detecting reagent, 50% H2SO4 in ethanol; heating time in 100 degrees C water bathe, 3 min; measuring wavelength, 490 nm.
  
  
  CONCLUSIONS
  The procedure for ESAC preparation is simple and well-designed, and the established method for ESAC can be used for the qualitative analysis of the G. lucidum related products.
authors.affiliation: School of Life Sciences and Technology, Beijing Institute of Technology, Beijing 100081, China.
authors.forename: Shu-ming
authors.initials: SM
authors.lastname: Huang
authors.forename: Xin-lin
authors.initials: XL
authors.lastname: Yang
authors.forename: Bang-wu
authors.initials: BW
authors.lastname: Wang
authors.forename: He-sun
authors.initials: HS
authors.lastname: Zhu
authors.forename: Jian-lan
authors.initials: JL
authors.lastname: Xu
mesh_terms: D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification*; D000431:Ethanol; D020940:Reishi / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*; D014315:Triterpenes / Q000032:analysis / Q000302:isolation & purification*
chemical_list: D004365:Drugs, Chinese Herbal; D014315:Triterpenes; D000431:Ethanol