title: [Monitoring indexes for early renal injury in the workers exposed to mercury].
abstract: OBJECTIVE
  To study the diagnostic method for early renal injury in the workers exposed to mercury (Hg).
  
  
  METHODS
  The contents of urinary Hg were determined by chemical method. Urinary microalbumin (mALB), beta(2)-microglodulin (beta(2)-MG) and retinol binding protein (RBP) levels were measured with total quantitative enzyme immunoassay. The activities of urinary N-acetyl-beta-D-glucosaminidase (NAG) and gamma-glutamyl transferase (gamma-GT) were determined by rate methods. Urinary creatinine (Cr) was measured by using picric acid method.
  
  
  RESULTS
  The levels of urinary BRP, beta(2)-MG, NAG and gamma-GT in exposed workers [(439.7 +/- 201.4), (141.4 +/- 56.3) micro g/g Cr and (12.3 +/- 5.7), (60.3 +/- 18.5) U/g Cr respectively] were significantly higher than those in controls (P < 0.05, P < 0.01). The levels were increased gradually with the increasing contents of urinary Hg. The positive detection rate for single or two combined indexes was rather lower whereas that for 4 combined indexes was as high as 85.5%. A positive correlation was noted between the contents of urinary Hg and urinary BRP, beta(2)-MG, NAG and gamma-GT (r: 0.466, 0.379, 0.323, 0.311, P < 0.05). Urinary RBP was correlated to urinary beta(2)-MG, NAG and gamma-GT (r: 0.362, 0.354, 0.332, P < 0.05).
  
  
  CONCLUSIONS
  Combined detection of urinary RBP, beta(2)-MG, NAG and gamma-GT is a sensitive method for the diagnosis of early renal injury in the workers exposed to Hg.
authors.affiliation: Shenyang No. 9th People's Hospital, Shenyang 110024, China.
authors.forename: Shan-zhuo
authors.initials: SZ
authors.lastname: Peng
authors.forename: Chun-sheng
authors.initials: CS
authors.lastname: Zhang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Hu
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Mingzhi
authors.initials: M
authors.lastname: Wei
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000118:Acetylglucosaminidase / Q000652:urine; D000328:Adult; D000419:Albuminuria / Q000652:urine; D003404:Creatinine / Q000652:urine; D005260:Female; D006801:Humans; D007668:Kidney / Q000293:injuries / Q000503:physiopathology; D007674:Kidney Diseases / Q000209:etiology / Q000652:urine*; D008297:Male; D008630:Mercury Poisoning / Q000150:complications*; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects*; D012177:Retinol-Binding Proteins / Q000652:urine; D005723:gamma-Glutamyltransferase / Q000652:urine
chemical_list: D012177:Retinol-Binding Proteins; D003404:Creatinine; D005723:gamma-Glutamyltransferase; D000118:Acetylglucosaminidase