[Development of magnetic biosorbents and their application in microbial antigen immunoassays].
The feasibility of rendering erythrocytes magnetic and thereby creating magnetic biosorbents (MB Ss) through room temperature exposure to 25-37% iron (II) sulfate solution for 48 +/- 2 h, followed by exposure to 15-35% ammonia water solution for 48 +/- 2 h (with drying after each procedure) was demonstrated. The feasibility of immobilizing ligands on magnetic erythrocytes (ME) and obtaining biological magnetic immunosorbents (BMISs) for further use in EIAs for plague and tularemia antigens was demonstrated. The sensitivity of EIAs involving BMISs amounted to 10 ng/ml and 100 microbial cells per 1 ml. The relative error did not exceed 8%.
Kal'noĭ
S M
SM
Stavropol Antiplague Institute, Stavropol, 355106 Russia.
Goncharov
V I
VI
Vasilenko
N F
NF
D000641:Ammonia; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D004912:Erythrocytes / Q000378:metabolism; D005296:Ferrous Compounds / Q000378:metabolism; D005604:Francisella tularensis / Q000276:immunology; D007118:Immunoassay / Q000379:methods*; D007164:Immunosorbents; D008024:Ligands; D008280:Magnetics; D012680:Sensitivity and Specificity; D013997:Time Factors; D015010:Yersinia pestis / Q000276:immunology
D000942:Antigens, Bacterial; D005296:Ferrous Compounds; D007164:Immunosorbents; D008024:Ligands; D000641:Ammonia