Isolation and molecular characterization of a novel type 3 reovirus from a child with meningitis.
Mammalian reoviruses are non-enveloped viruses that contain a segmented, double-stranded RNA genome. Reoviruses infect most mammalian species, although infection with these viruses in humans is usually asymptomatic. We report the isolation of a novel reovirus strain from a 6.5-week-old child with meningitis. Hemagglutination and neutralization assays indicated that the isolate is a serotype 3 strain, leading to the designation T3/Human/Colorado/1996 (T3C/96). Sequence analysis of the T3C/96 S1 gene segment, which encodes the viral attachment protein, sigma 1, confirmed the serotype assignment for this strain and indicated that T3C/96 is a novel reovirus isolate. T3C/96 is capable of systemic spread in newborn mice after peroral inoculation and produces lethal encephalitis. These results suggest that serotype 3 reoviruses can cause meningitis in humans.
Tyler
Kenneth L
KL
Department of Neurology, University of Colorado Health Science Center, Denver, Colorado 80262, USA. ken.tyler@uchsc.edu.
Barton
Erik S
ES
Ibach
Maria L
ML
Robinson
Christine
C
Campbell
Jacquelyn A
JA
O'Donnell
Sean M
SM
Valyi-Nagy
Tibor
T
Clarke
Penny
P
Wetzel
J Denise
JD
Dermody
Terence S
TS
D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D002460:Cell Line; D002555:Cerebrospinal Fluid / Q000821:virology; D005260:Female; D006367:HeLa Cells; D006801:Humans; D007223:Infant; D012089:Mammalian orthoreovirus 3 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D008587:Meningitis, Viral / Q000821:virology*; D051379:Mice; D008969:Molecular Sequence Data; D012088:Reoviridae Infections / Q000821:virology*; D017422:Sequence Analysis, DNA; D012703:Serotyping
D036022:Capsid Proteins; C031728:sigma 1 protein, reovirus