title: Cell response to dextran-derivatised iron oxide nanoparticles post internalisation.
abstract: Magnetic nanoparticles have been used for many years as magnetic resonance imaging contrast agents. Despite the fact that there are currently several dextran-coated iron oxide nanoparticles are in preclinical and clinical use, there is very little information available concerning the influence such particles have on cells in culture. The prerequisite for particles employed as contrast agents is capture and subsequent uptake by cells. This study involved the use of magnetic nanoparticles synthesised and derivatised with dextran, compared to similar underivatised plain particles. The influence in vitro was assessed using human dermal fibroblasts and various techniques to observe cell-particles interaction, including light and fluorescence microscopy, scanning and transmission electron microscopy. The results indicate that although both the uncoated and the dextran-derivatised particles are uptaken into the cell, the derivatised particles induce alterations in cell behaviour and morphology distinct from the plain particles, suggesting that cell response is dependent on the particles coating.
authors.affiliation: Centre for Cell Engineering, University of Glasgow, Joseph Black Building, IBLS University Avenue, Glasgow G12 8QQ, UK. catherine.berry @bio.gla.ac.uk
authors.forename: Catherine Cecilia
authors.initials: CC
authors.lastname: Berry
authors.forename: Stephen
authors.initials: S
authors.lastname: Wells
authors.forename: Stuart
authors.initials: S
authors.lastname: Charles
authors.forename: Gregor
authors.initials: G
authors.lastname: Aitchison
authors.forename: Adam S G
authors.initials: AS
authors.lastname: Curtis
mesh_terms: D000327:Adsorption; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000493:pharmacokinetics* / Q000494:pharmacology; D003911:Dextrans / Q000138:chemical synthesis / Q000493:pharmacokinetics* / Q000494:pharmacology; D005290:Ferric Compounds / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size
chemical_list: D020099:Coated Materials, Biocompatible; D003911:Dextrans; D005290:Ferric Compounds; C000499:ferric oxide