title: Habenula lesions cause impaired cognitive performance in rats: implications for schizophrenia.
abstract: Cognitive impairment is a prominent feature of schizophrenia. Currently there is no well-accepted explanation of the aetiology of this disorder, but recent evidence indicates that dysfunction of the habenula may be involved. We therefore examined whether habenula lesions in Sprague-Dawley rats cause behavioural changes resembling those of schizophrenia. Rats received either habenula lesions, a sham operation or a small lesion of the overlying dorsal hippocampus as a check that effects observed were not due to incidental damage to this structure. As there are alterations of social behaviour, sensorimotor gating and cognition in schizophrenia, we examined comparable behaviours. Social interaction time was measured during a 5-min encounter with a novel juvenile conspecific. Prepulse inhibition of an acoustic startle response, as an index of sensorimotor gating, was measured with prepulses of various amplitudes, and spatial cognitive performance was assessed in the Morris water maze task. Histological analysis showed that habenula lesions substantially damaged both medial and lateral habenula bilaterally while largely sparing neighbouring structures. Assay of choline acetyltransferase (ChAT) in the interpeduncular nucleus terminal region of the habenulo-interpeduncular tract, showed marked reduction (by 80%) in habenula-lesioned animals. Habenula-lesioned rats, but not the control group with small dorsal hippocampus lesions, showed marked impairment of Morris maze performance compared to the sham-operated control group. Social interaction time and prepulse inhibition were not significantly altered in either lesion group. The results are consistent with a role of the habenula in cognition, and with the view that pathology of the habenula may contribute to the cognitive impairments of schizophrenia.
authors.affiliation: NS Research, WSJ-386. 262, Novartis Institutes for BioMedical Research, CH-4002 Basel, Switzerland.
authors.forename: Lucas
authors.initials: L
authors.lastname: Lecourtier
authors.forename: Hans C
authors.initials: HC
authors.lastname: Neijt
authors.forename: Peter H
authors.initials: PH
authors.lastname: Kelly
mesh_terms: D000161:Acoustic Stimulation / Q000379:methods; D000818:Animals; D001522:Behavior, Animal; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003072:Cognition Disorders / Q000209:etiology / Q000503:physiopathology*; D004195:Disease Models, Animal; D004307:Dose-Response Relationship, Radiation; D019262:Habenula / Q000293:injuries / Q000502:physiology*; D007398:Interpersonal Relations; D008297:Male; D018782:Maze Learning / Q000502:physiology; D009433:Neural Inhibition / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013216:Reflex, Startle / Q000502:physiology; D012559:Schizophrenia / Q000150:complications / Q000503:physiopathology*; D013550:Swimming / Q000502:physiology; D013997:Time Factors
chemical_list: D002795:Choline O-Acetyltransferase