Molecular characterization of Egyptian human and anima Echinococcus granulosus isolates by RAPD-PCR technique.
Five primers of known, but arbitrary nucleotide sequence (OPH-03, OPH-05, OPH-12, OPH-15, OPH-18) were used to detect genetic variability in Egyptian human, camel and pig E. granulosus isolates. OPH-03, OPH-05 & OPH-15 proved useful as genetic markers of strain variation, while OPH-12 and OPH-18 allowed distinction at the genus level i.e. diversified from Cysticercus tenuicollis. OPH-03 was the most effective giving sharp distinct banding pattern and the least values of similarity coefficients. Some variations were detected within E. granulosus isolates from the same host. The level of heterogeneity was low in three of the human isolates, camel and pig strains. Individual variation was detectable within other 3 human isolates. Human and camel isolates were the most related pair, having similar patterns and the highest similarity coefficients. The study implies that human cases in Egypt are of the camel/dog strain, and camels are important hosts for the transmission of human hydatidosis.
Azab
Magda E
ME
Department of Parasitology, Faculty of Medicine, Ain Shams University, Cairo 11566.
Bishara
Sawsan A
SA
Helmy
Hanan
H
Oteifa
Nihad M
NM
El-Hoseiny
Laila M
LM
Ramzy
Reda M R
RM
Ahmed
Mona A
MA
D000818:Animals; D002162:Camelus / Q000469:parasitology; D018509:DNA, Helminth / Q000032:analysis; D004443:Echinococcosis / Q000469:parasitology / Q000635:transmission*; D004446:Echinococcus / Q000235:genetics*; D004534:Egypt; D006801:Humans; D011634:Public Health; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology; D015047:Zoonoses
D018509:DNA, Helminth