title: Reduced L-selectin (CD62LLow) expression identifies tumor-specific type 1 T cells from lymph nodes draining an autologous tumor cell vaccine.
abstract: Reduced expression of CD62L can identify tumor-specific T cells in lymph nodes draining murine tumors. Here, we examined whether this strategy could isolate tumor-specific T cells from vaccinated patients. Tumor vaccine-draining lymph node (TVDLN) T cells of seven patients were separated into populations with reduced (CD62LLow) or high levels of CD62L (CD62LHigh). Effector T cells generated from CD62LLow cells maintained or enriched the autologous tumor-specific type 1 cytokine response compared to unseparated TVDLN T cells in four of four patients showing tumor-specific cytokine secretion. Interestingly, effector T cells generated from CD62LLow or CD62LHigh TVDLN were polarized towards a dominant type 1 or type 2 cytokine profile, respectively. For CD62LLow T cells the type 1 cytokine profile appeared determined prior to culture. Since a tumor-specific type 1 cytokine profile appears critical for mediating anti-tumor activity in vivo, this approach might be used to isolate T cells for adoptive immunotherapy.
authors.affiliation: Laboratory of Molecular and Tumor Immunology, Robert W. Franz Cancer Research Center, Earle A. Chiles Research Institute, Providence Portland Medical Center, Portland, OR, USA.
authors.forename: Sybren L
authors.initials: SL
authors.lastname: Meijer
authors.forename: Annemieke
authors.initials: A
authors.lastname: Dols
authors.forename: Hong-Ming
authors.initials: HM
authors.lastname: Hu
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Chu
authors.forename: Pedro
authors.initials: P
authors.lastname: Romero
authors.forename: Walter J
authors.initials: WJ
authors.lastname: Urba
authors.forename: Bernard A
authors.initials: BA
authors.lastname: Fox
mesh_terms: D019496:Cancer Vaccines / Q000276:immunology*; D002292:Carcinoma, Renal Cell / Q000276:immunology* / Q000628:therapy; D006801:Humans; D016219:Immunotherapy, Adoptive; D007371:Interferon-gamma / Q000096:biosynthesis; D015848:Interleukin-5 / Q000096:biosynthesis; D007680:Kidney Neoplasms / Q000276:immunology* / Q000628:therapy; D019041:L-Selectin / Q000032:analysis*; D008198:Lymph Nodes / Q000276:immunology*; D008545:Melanoma / Q000276:immunology* / Q000628:therapy; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D019496:Cancer Vaccines; D015848:Interleukin-5; D019041:L-Selectin; D007371:Interferon-gamma