Enzyme production by the mycoparasite Verticillium biguttatum against Rhizoctonia solani.
Verticillium biguttatum, a mycoparasite of the ubiquitous soil-borne plant pathogen Rhizoctonia solani, excreted chitinase and beta-1,3-glucanase into liquid medium when grown on laminarin and chitin, respectively. Neither chitinase nor beta-1,3-glucanase was produced by the mycoparasite when grown on cell walls of two isolates of R. solani representing anastomosis groups (AG)-3 and AG-8. Extracellular protease was induced by growth on cell walls of the pathogen, whereas beta-1,3-glucanase and chitinase were produced bound to the cell wall of V. biguttatum. This is the first report of chitinase, beta-1,3-glucanase and protease production by V. biguttatum. These enzymes may play a previously unforeseen role in dissolving and penetrating the cell walls of R. solani.
McQuilken
Mark P
MP
Department of Plant Biology, Scottish Agricultural College, Ayr Campus, Auchincruive Estate, Ayr KA6 5HW, UK. m.mcquilken@au.sac.ac.uk
Gemmell
Jacqueline
J
D002473:Cell Wall / Q000378:metabolism; D002688:Chitinases / Q000378:metabolism*; D003470:Culture Media; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000378:metabolism*; D006790:Host-Parasite Interactions; D025282:Mycelium / Q000201:enzymology; D010572:Pest Control, Biological; D012232:Rhizoctonia / Q000254:growth & development*; D020172:Verticillium / Q000201:enzymology* / Q000254:growth & development
D003470:Culture Media; D002688:Chitinases; D004693:Glucan Endo-1,3-beta-D-Glucosidase