title: Tuning the setup of sputter-coated multilayers in nanocluster-based signal-enhancing biochips for optimal performance in protein and DNA assays.
abstract: In biochip development two issues are critical: stable and specific immobilization of the ligand and achievement of high signal-to-background ratio. In this work we have addressed these issues for the development of biochips, produced by sputtering multilayers of thin metal films, metal oxides, and metal nitrides (tens to hundreds of nanometers thick) onto glass wafers. Optimized surfaces have shown good results in genomic and proteomic experiments with biochips based on surface-enhanced fluorescence and absorption techniques.
authors.affiliation: Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.
authors.forename: Viatcheslav
authors.initials: V
authors.lastname: Matyushin
authors.forename: Arminder
authors.initials: A
authors.lastname: Gandhum
authors.forename: Norbert
authors.initials: N
authors.lastname: Stich
authors.forename: Thomas
authors.initials: T
authors.lastname: Schalkhammer
authors.forename: Wilfred R
authors.initials: WR
authors.lastname: Hagen
authors.forename: Christian
authors.initials: C
authors.lastname: Mayer
mesh_terms: D000327:Adsorption; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry*; D003460:Crystallization / Q000379:methods*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005740:Gases; D006358:Hot Temperature; D008422:Materials Testing; D008567:Membranes, Artificial; D008670:Metals / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D005740:Gases; D008567:Membranes, Artificial; D008670:Metals