Mouse monoclonal IgA binds to the galectin-3/Mac-2 lectin from mouse macrophage cell lines.
The beta-galactoside-binding S-type lectin galectin-3/Mac-2, expressed among several other cell types on activated macrophages, is known to bind IgE, but not other Ig classes. We report in this paper that the single major constituent in a detergent lysate from the J774 mouse macrophage cell line bound to a mouse monoclonal IgA-affinity column. This fraction has been identified by mass spectrometry analysis as galectin-3. Binding of both mouse IgA and IgE to galectin-3 coated plates was inhibited by lactose and asialofetuin. Furthermore, three different monoclonal IgAs bound also to purified recombinant hamster galectin-3 coated plates in a concentration dependent manner. The potential functional significance of IgA binding to galectin-3 within macrophages and possibly other cell types is discussed.
Reljic
Rajko
R
Department of Oral Medicine and Pathology, Guy's Campus of King's College London, Guy's Tower Floor 28, Guy's Hospital, London SE1 9RT, UK.
Crawford
Carol
C
Challacombe
Stephen
S
Ivanyi
Juraj
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002241:Carbohydrates / Q000276:immunology; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D037502:Galectin 3 / Q000276:immunology* / Q000302:isolation & purification; D007070:Immunoglobulin A / Q000276:immunology*; D008024:Ligands; D008264:Macrophages / Q000276:immunology*; D051379:Mice
D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D037502:Galectin 3; D007070:Immunoglobulin A; D008024:Ligands