Polyamine metabolism in a member of the phylum Microspora (Encephalitozoon cuniculi): effects of polyamine analogues.
The uptake, biosynthesis and catabolism of polyamines in the microsporidian parasite Encephalitozoon cuniculi are detailed with reference to the effects of oligoamine and arylamine analogues of polyamines. Enc. cuniculi, an intracellular parasite of mammalian cells, has both biosynthetic and catabolic enzymes of polyamine metabolism, as demonstrated in cell-free extracts of mature spores. The uptake of polyamines was measured in immature, pre-emergent spores isolated from host cells by Percoll gradient. Spermine was rapidly taken up and metabolized to spermidine and an unknown, possibly acetamidopropanal, by spermidine/spermine N(1)-acetyltransferase (SSAT) and polyamine oxidase (PAO). Most of the spermidine and the unknown product were found in the cell incubation medium, indicating they were released from the cell. bis(Ethyl) oligoamine analogues of polyamines, such as SL-11144 and SL-11158, as well as arylamine analogues [BW-1, a bis(phenylbenzyl) 3-7-3 analogue] blocked uptake and interconversion of spermine at micromolar levels and, in the case of BW-1, acted as substrate for PAO. The Enc. cuniculi PAO activity differed from that found in mammalian cells with respect to pH optimum, substrate specificity and sensitivity to known PAO inhibitors. SL-11158 inhibited SSAT activity with a mixed type of inhibition in which the analogue had a 70-fold higher affinity for the enzyme than the natural substrate, spermine. The interest in Enc. cuniculi polyamine metabolism and the biochemical effects of these polyamine analogues is warranted since they cure model infections of Enc. cuniculi in mice and are potential candidates for human clinical trials.
Bacchi
Cyrus J
CJ
Haskins Laboratories and Department of Biology, Pace University, New York, NY 10038, USA.
Rattendi
Donna
D
Haskins Laboratories and Department of Biology, Pace University, New York, NY 10038, USA.
Faciane
Evangeline
E
Haskins Laboratories and Department of Biology, Pace University, New York, NY 10038, USA.
Yarlett
Nigel
N
Department of Chemistry, Pace University, New York, NY 10038, USA.|Haskins Laboratories and Department of Biology, Pace University, New York, NY 10038, USA.
Weiss
Louis M
LM
Departments of Medicine and Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Frydman
Benjamin
B
SLIL Biomedical Corp., Madison, WI 53711, USA.
Woster
Patrick
P
Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48202, USA.
Wei
Benjamin
B
Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48202, USA.
Marton
Laurence J
LJ
SLIL Biomedical Corp., Madison, WI 53711, USA.
Wittner
Murray
M
Departments of Medicine and Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
D000123:Acetyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism; D000818:Animals; D000981:Antiprotozoal Agents / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D009670:Encephalitozoon cuniculi / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000037:antagonists & inhibitors / Q000378:metabolism; D011073:Polyamines / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D011817:Rabbits; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D013379:Substrate Specificity; D000097667:Polyamine Oxidase; D000099228:Diamine N-Acetyltransferase
D000123:Acetyltransferases; D000981:Antiprotozoal Agents; D004791:Enzyme Inhibitors; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011073:Polyamines; D013095:Spermidine; D013096:Spermine; D000097667:Polyamine Oxidase; D000099228:Diamine N-Acetyltransferase; C465646:SL-11158