[The antitussive and de-sputum essential substance from the root of Gerbera piloselloides and its analytical compositions].
OBJECTIVE
To study the chemical constituents of the essential substance from the root of Gerbera piloselloides and its antitussive and de-sputum effects.


METHODS
The essential substance (G4) was extracted from the root by alcohol and ethyl acetate, then it was separated by silica gel column eluted by the mixture of ethyl acetate and petroleum ether (5:95). Its chemical components were separated and identified by GC-MS. Its antitussive and de-sputum effect was tested by mice.


RESULTS
4 main peaks were separated and identified by GS-MS. They are beta-caryophyllene (15.160%), caryophyllene oxide (21.140%), aristolenepoxide (2.673%) and 6-acetyl-2,2-dimethyl-8(3-methyl-2-butenyl)-2H-chromoene (60.077%) respectively. Its antitussive and de-sputum effect was prominent when the mice was given G4 2,000 mg.kg-1 ig.


CONCLUSIONS
Itis the first time that the antitussive and de-sputum essential substance was separated from the root of Gerbera piloselloides and its main compositions were analyzed.
Tang
Xiao-jiang
XJ
Zhongshan University, Guangdong Provincial Center for Occupational Disease Prevention and Treatment, Guangzhou, Guangdong, China. Chao-zhu@163.com
Huang
Hua-rong
HR
Fang
Tie-zheng
TZ
Yang
Cui-ping
CP
Zhang
Yuan
Y
Wu
Ban-hua
BH
Xu
Shi-bo
SB
D000818:Animals; D000996:Antitussive Agents / Q000302:isolation & purification / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D002867:Chromones / Q000302:isolation & purification* / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005100:Expectorants / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D051379:Mice; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000494:pharmacology
C488007:6-acetyl-2,2-dimethyl-8-(3-methyl-2-butenyl)-2H-chromone; D000996:Antitussive Agents; D002867:Chromones; D004365:Drugs, Chinese Herbal; D005100:Expectorants; D009822:Oils, Volatile; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; C024714:caryophyllene