title: Organization of the mitochondrial genome of Antarctic krill Euphausia superba (Crustacea: Malacostraca).
abstract: We determined the nearly complete DNA sequence of the mitochondrial genome of Antarctic krill Euphausia superba (Crustacea: Malacostraca), one of the most ecologically and commercially important zooplankters in Antarctic waters. All of the genome sequences were purified by gene amplification using long polymerase chain reaction (PCR), and the products were subsequently used as templates for either direct sequencing using a primer-walking strategy or nested PCR with crustacea-versatile primers. Although we were unable to determine a portion of the genome owing to technical difficulties, the sequenced position, 14,606 bp long, contained all of the 13 protein-coding genes, 19 of the 22 transfer RNA genes, and the large subunit as well as a portion of the small subunit ribosomal RNA genes. Gene rearrangement was observed for 3 transfer RNA genes (tRNACys, tRNATyr, and tRNATrp) and the 2 leucine tRNA genes.
authors.affiliation: Ocean Research Institute, University of Tokyo, Tokyo 164-8639, Japan. ryuji@ori.u-tokyo.ac.jp
authors.forename: Ryuji J
authors.initials: RJ
authors.lastname: Machida
authors.forename: Masaki U
authors.initials: MU
authors.lastname: Miya
authors.forename: Mitsugu M
authors.initials: MM
authors.lastname: Yamauchi
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Nishida
authors.forename: Shuhei
authors.initials: S
authors.lastname: Nishida
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000864:Antarctic Regions; D001482:Base Composition; D020029:Base Pairing; D001483:Base Sequence; D016000:Cluster Analysis; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D033201:Euphausiacea / Q000235:genetics*; D023061:Gene Order; D015321:Gene Rearrangement / Q000235:genetics*; D008969:Molecular Sequence Data; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D012343:RNA, Transfer