title: High-performance thin-layer chromatography with a derivatization procedure, a suitable method for the identification and the quantitation of busulfan in various pharmaceutical products.
abstract: Busulfan is an alkylating agent widely used in combination chemotherapy regimens followed by allogeneic or autologous hematopoietic stem cell transplantation (HSCT). We present a rapid method for assaying busulfan in pharmaceutical preparations using high-performance thin-layer chromatography (HPTLC) and derivatization with 4-nitrobenzylpyridine. The method is accurate and precise and allows quantitation of busulfan in aqueous solutions from 100 to 500 microg/ml. It is suitable for identification. quantitation and stability studies of busulfan in pharmaceutical products, i.e. capsules or infusion bags prepared in a hospital pharmacy.
authors.affiliation: Department of Clinical Pharmacy, Institut Gustave Roussy, 39 Rue Camille Desmoulins, 94800 Villejuif, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Bouligand
authors.forename: Angelo
authors.initials: A
authors.lastname: Paci
authors.forename: Lionel
authors.initials: L
authors.lastname: Mercier
authors.forename: Gilles
authors.initials: G
authors.lastname: Vassal
authors.forename: Philippe
authors.initials: P
authors.lastname: Bourget
mesh_terms: D002066:Busulfan / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000737:chemistry
chemical_list: D004364:Pharmaceutical Preparations; D002066:Busulfan