Liquid chromatographic method for the determination of rizatriptan in human plasma.
A high-performance liquid chromatographic (HPLC) method with fluorescence detection has been developed for the determination of rizatriptan in human plasma. Following a single-step liquid-liquid extraction with methyl tertiarybutyl ether, the analytes were separated using a mobile phase consisting of 0.05% (v/v) triethylamine in water (adjusting to pH 2.75 with 85% phosphoric acid) and acetonitrile (92:8, v/v). Fluorescence detection was performed at an excitation wavelength of 225nm and an emission wavelength of 360nm. The linearity for rizatriptan was within the concentration range of 0.5-50ng/ml. The intra- and inter-day precisions of the method were not more than 8.0%. The lower limit of quantification (LLOQ) was 0.5ng/ml for rizatriptan. The method was sensitive, simple and repeatable enough to be used in pharmacokinetic studies.
Chen
Jun
J
Department of Pharmaceutics, School of Pharmacy, Fudan University, 138 Yi Xue Yuan Road, Shanghai 200032, PR China.
Jiang
Xinguo
X
Jiang
Wenming
W
Mei
Ni
N
Gao
Xiaoling
X
Zhang
Qizhi
Q
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017366:Serotonin Receptor Agonists / Q000097:blood* / Q000493:pharmacokinetics; D013050:Spectrometry, Fluorescence; D014230:Triazoles / Q000097:blood* / Q000493:pharmacokinetics; D014363:Tryptamines
D017366:Serotonin Receptor Agonists; D014230:Triazoles; D014363:Tryptamines; C093622:rizatriptan