Optimized chimeragenesis; creating diverse p450 functions.
A drawback to generating chimeric proteins by chimeragenesis, especially when the "parent" proteins share low sequence identity, is that unfolded proteins frequently result. In this issue of Chemistry & Biology, Arnold and coworkers report their use of the SCHEMA algorithm to effectively predict ideal hybrids of cytochromes p450.
Bernhardt
Rita
R
Department of Biochemistry, PO Box 15 11 50, Saarland University, D-66041 Saarbrücken, Germany.
D000465:Algorithms; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004795:Enzyme Stability; D009154:Mutation / Q000235:genetics; D015202:Protein Engineering; D017510:Protein Folding; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D011993:Recombinant Fusion Proteins; D003577:Cytochrome P-450 Enzyme System