title: Effect of enamel matrix derivative (Emdogain) on bone defects in rabbit tibias.
abstract: The aim of this study was to assess the effect of an enamel matrix derivative (Emdogain, Biora, AB, Malmö, Sweden) on bone healing. Ten New Zealand rabbits, weighing about 2.5 kg, were used. One 8-mm bone defect was created in each tibia. The defect on the right leg was filled with Emdogain, whereas the defect on the opposite leg was left unfilled as control. A total of 20 defects were created. Five rabbits each were killed at 4 and 8 weeks with an overdose of Tanax. Block sections containing the defects were retrieved and the specimens processed for light microscopy examination. The slides were stained with acid and basic fuchsin and toluidine blue. Histologically, no differences were noted in both groups at each observation period; in the test group, remnants of the implanted Emdogain were not present at 4 weeks. Newly formed bone was detectable in both groups at all observation times. At 8 weeks, both groups showed mature bone, and in the test group the material implanted was not visible. No inflammatory cells were visible in both groups. In conclusion, our results indicate that Emdogain implanted in bone defects is fully resorbed after 4 to 8 weeks and does not adversely affect bone formation.
authors.affiliation: Dental School, University of Chieti, Chieti, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Cornelini
authors.forename: Antonio
authors.initials: A
authors.lastname: Scarano
authors.forename: Maurizio
authors.initials: M
authors.lastname: Piattelli
authors.forename: Sebastiano
authors.initials: S
authors.lastname: Andreana
authors.forename: Ugo
authors.initials: U
authors.lastname: Covani
authors.forename: Alessandro
authors.initials: A
authors.lastname: Quaranta
authors.forename: Adriano
authors.initials: A
authors.lastname: Piattelli
mesh_terms: D000818:Animals; D001557:Benzenesulfonates; D001847:Bone Diseases / Q000473:pathology / Q000601:surgery*; D001857:Bone Matrix / Q000473:pathology; D001861:Bone Regeneration / Q000187:drug effects; D018786:Bone Substitutes / Q000627:therapeutic use*; D004396:Coloring Agents; D003746:Dental Enamel Proteins / Q000627:therapeutic use*; D004195:Disease Models, Animal; D010006:Osteoblasts / Q000473:pathology; D011817:Rabbits; D012394:Rosaniline Dyes; D013977:Tibia / Q000473:pathology / Q000601:surgery*; D013997:Time Factors; D014048:Tolonium Chloride; D014945:Wound Healing / Q000187:drug effects
chemical_list: D001557:Benzenesulfonates; D018786:Bone Substitutes; D004396:Coloring Agents; D003746:Dental Enamel Proteins; D012394:Rosaniline Dyes; C109069:enamel matrix proteins; D014048:Tolonium Chloride; C025485:basic fuchsin; C086337:acid fuchsin