title: New Polyacetylenes, DGAT inhibitors from the roots of Panax ginseng.
abstract: The petroleum ether extract of Panax ginseng showed a significant inhibition of the diacylglycerol acyltransferase (DGAT) enzyme from rat liver microsomes. Bioactivity-guided fractionation led to the isolation of two new polyacetylenic compounds, (9 R,10 S)-epoxyheptadecan-4,6-diyn-3-one ( 1) and 1-methoxy-(9 R,10 S)-epoxyheptadecan-4,6-diyn-3-one ( 2). Their chemical structures were elucidated on the basis of spectroscopic evidence and asymmetric synthesis. IC50 values of 9 microg/mL ( 1) and 32 microg/mL ( 2) were obtained.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Taejon, Korea.
authors.forename: Seung Woong
authors.initials: SW
authors.lastname: Lee
authors.forename: Koanhoi
authors.initials: K
authors.lastname: Kim
authors.forename: Mun-Chual
authors.initials: MC
authors.lastname: Rho
authors.forename: Mi Yeon
authors.initials: MY
authors.lastname: Chung
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
authors.forename: Sangku
authors.initials: S
authors.lastname: Lee
authors.forename: Hyun Sun
authors.initials: HS
authors.lastname: Lee
authors.forename: Young Kook
authors.initials: YK
authors.lastname: Kim
mesh_terms: D000114:Acetylene / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology* / Q000627:therapeutic use; D000217:Acyltransferases / Q000037:antagonists & inhibitors* / Q000187:drug effects*; D000818:Animals; D051048:Diacylglycerol O-Acyltransferase; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D020128:Inhibitory Concentration 50; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D005894:Panax; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots; D011108:Polymers / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D053279:Polyynes; D051381:Rats
chemical_list: D004791:Enzyme Inhibitors; D010936:Plant Extracts; D011108:Polymers; D053279:Polyynes; D000217:Acyltransferases; D051048:Diacylglycerol O-Acyltransferase; D000114:Acetylene