title: [The effects of chitosan on different-derived fibroblasts].
abstract: OBJECTIVE
  To investigate the effects of chitosan on the biological activities of the fibroblasts derived from different tissues.
  
  
  METHODS
  The biological activities of the fibroblasts derived from different tissues were evaluated with a MTT method for fibroblast proliferation, photic and electronic microscope for morphologic and subcellular structure, 3H-proline uptake method for collagen secretion and ELISA box for the secretion of TGF-beta 1, FGF-AB, and IL-8.
  
  
  RESULTS
  This study showed that the chitosan inhabited the proliferation of the fibroblasts and the secretion of the TGF-beta 1, FGF-AB and collagen of the fibroblasts with a dose-depended manner in the normal skin, hypertrophic scar and keloid groups, but it stimulated the IL-8. However, there were no significant differences among the three groups (P > 0.05).
  
  
  CONCLUSIONS
  The chitosan could inhibit the growth, proliferation, biosynthesis and secretion of the fibroblasts, and it may be used to treat different scars.
authors.affiliation: Department of Plastic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Jing-De
authors.initials: JD
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Xing
authors.forename: Mei-qing
authors.initials: MQ
authors.lastname: Sun
authors.forename: Jian-xing
authors.initials: JX
authors.lastname: Song
authors.forename: Tian-xiang
authors.initials: TX
authors.lastname: Ouyang
mesh_terms: D000293:Adolescent; D000328:Adult; D002455:Cell Division / Q000187:drug effects; D002686:Chitin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D048271:Chitosan; D004305:Dose-Response Relationship, Drug; D005260:Female; D005346:Fibroblast Growth Factors / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D006490:Hemostatics / Q000494:pharmacology; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D010446:Peptide Fragments / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D006490:Hemostatics; D016209:Interleukin-8; D010446:Peptide Fragments; D016212:Transforming Growth Factor beta; D002686:Chitin; D005346:Fibroblast Growth Factors; D048271:Chitosan; C052888:fibroblast growth factor (1-10)