title: Electrochemical determination of lead and glutathione in a plant cell culture.
abstract: In this work we established differential pulse anodic stripping voltammetry (DPASV) as the tool for analysis of lead in the plant cell culture. For the cultivation procedure, lead in Pb(II)-ethylenediaminetetraacetic acid (Pb-EDTA) chelate has been used. The detection limit of lead was found at 500 pM in phosphate buffer (pH 5.5), and 100 nM in prepared cells intracellular extract (20 pg Pb(II)/mg cells). For determination of cysteine-rich peptides, voltammetry in differential mode (DPV) in cobalt(III)-containing ammonia buffer (Brdicka reaction) was used. In this short communication, we present suitable voltammetric techniques for the physiological study of lead and thiols in plant cell culture.
authors.affiliation: Department of Botany and Plant Physiology, Mendel University of Agriculture and Forestry, Zemedelska 1, 613 00 Brno, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Vacek
authors.forename: Jirí
authors.initials: J
authors.lastname: Petrek
authors.forename: René
authors.initials: R
authors.lastname: Kizek
authors.forename: Ladislav
authors.initials: L
authors.lastname: Havel
authors.forename: Borivoj
authors.initials: B
authors.lastname: Klejdus
authors.forename: Libuse
authors.initials: L
authors.lastname: Trnková
authors.forename: Frantisek
authors.initials: F
authors.lastname: Jelen
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D004563:Electrochemistry / Q000379:methods*; D005978:Glutathione / Q000032:analysis* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007854:Lead / Q000032:analysis* / Q000378:metabolism; D028222:Picea / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000032:analysis* / Q000378:metabolism
chemical_list: D013438:Sulfhydryl Compounds; D007854:Lead; D005978:Glutathione