title: [Effects of varglaucocalyx on c-fos gene expression during global myocardial ischemia-reperfusion in rat].
abstract: OBJECTIVE
  To determine the effects of Varglaucocalyx on c-fos gene expression during global myocardial ischemia-reperfusion.
  
  
  METHODS
  Forty Wistar rats were divided into 5 groups: group N as control; group CN as ischemia-reperfusion control and group XH, XM and XL treated with Varglaucocalyx 5%, 1%, 0.5% respectively prior to ischemia-reperfusion. The isolated rat hearts were perfused in condition of constant temperature and pressure, and then the left ventricular myocardiums were extracted for use. The expression of c-fos protein was detected by immunochemical method. The expression of c-fos protein were quantified by using computer image analysis system.
  
  
  RESULTS
  Compared with the values of group N, protein expressions relative area of c-fos gene (PERA) were increased significantly in group CN, XH, XM, XL(P < 0.01), but decreased significantly in group XH, XM, XL, compared with those of group CN (P < 0.05). The PERA of c-fos gene in group XM, XL were significantly lower than in group XH (P < 0.01), and the PERA of c-fos gene in group XM were lower than in group XL(P < 0.05).
  
  
  CONCLUSIONS
  Varglaucocalyx can effectively depress the expression of c-fos gene in myocardium which may account for its protection against myocardial ischemia-reperfusion injury, and the middle and the low concentrations of Varglaucocalyx are more effective than the high concentrations.
authors.affiliation: No. 202 Hospital Of PLA, Shenyang 110003, Liaoning, China.
authors.forename: Lan-di
authors.initials: LD
authors.lastname: Liu
authors.forename: Li-ka
authors.initials: LK
authors.lastname: Ye
authors.forename: Dong-jun
authors.initials: DJ
authors.lastname: Pan
authors.forename: Yan-duo
authors.initials: YD
authors.lastname: Jiang
authors.forename: Tie-zheng
authors.initials: TZ
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
mesh_terms: D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D016762:Genes, fos; D037602:Isodon / Q000737:chemistry; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000209:etiology / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002316:Cardiotonic Agents; D004365:Drugs, Chinese Herbal; D016760:Proto-Oncogene Proteins c-fos