title: Effect of ischemia/reperfusion on telomere length and CDKI genes expression in a concordant ex-vivo hemoperfusion model of primate kidneys.
abstract: OBJECTIVE
  The telomere (T) length, p21(WAF1/CIP1) and p27(Kip1) cyclin dependent kinase inhibitor (CDKI) genes are considered the markers of cell senescence and DNA damage. The aim of the study was to evaluate the influence of renal ischemia/reperfusion (I/R) on the value of above-mentioned markers.
  
  
  METHODS
  13 Macaque cynomolgus monkey kidneys were harvested and placed in Eurocollins solution. 9 kidneys were ex-vivo perfused with human blood and 4 kidneys were not perfused at all (control group). Tissue expression of p21(WAF1/CIP1) and p27(Kip1) was evaluated immunohistochemically and the T lengths were measured by southern blotting technique.
  
  
  RESULTS
  Significantly higher levels of p21 and p27 were expressed by the glomeruli (p = 0.001 and 0.0001), tubules (p = 0.0065 and 0.0006) and interstitial cells (p = 0.0017 and 0.0022, respectively) of the xenoperfused kidneys. The mean T length was higher in the control group (5.56 +/- 0.60 kbp) than in the study group kidneys (5.46 +/- 0.36 kbp) (P = NS).
  
  
  CONCLUSIONS
  Renal I/R is associated with telomere shortening and an over-expression of p21 and p27 genes indicating substantial DNA damage and/or accelerated tissue senescence.
authors.affiliation: Transplantation Centre, University Clinic Ulm, Ulm, Germany. hubert.schelzig@medizin.uni-ulm.de
authors.forename: Hubert
authors.initials: H
authors.lastname: Schelzig
authors.forename: Archil B
authors.initials: AB
authors.lastname: Chkhotua
authors.forename: Peter
authors.initials: P
authors.lastname: Wiegand
authors.forename: Stephan
authors.initials: S
authors.lastname: Grosse
authors.forename: Simone
authors.initials: S
authors.lastname: Reis
authors.forename: Martina
authors.initials: M
authors.lastname: Art
authors.forename: Dietmar
authors.initials: D
authors.lastname: Abendroth
mesh_terms: D000818:Animals; D016203:CDC2 Protein Kinase / Q000235:genetics*; D005786:Gene Expression Regulation; D006464:Hemoperfusion; D007511:Ischemia; D007668:Kidney / Q000098:blood supply* / Q000166:cytology / Q000502:physiology*; D007678:Kidney Glomerulus / Q000166:cytology; D008252:Macaca fascicularis; D023421:Models, Animal; D009926:Organ Preservation; D015424:Reperfusion; D016615:Telomere / Q000648:ultrastructure*
chemical_list: D016203:CDC2 Protein Kinase