Enrichment of cardiolipin content throughout the purification procedure of photosystem II.
Photosystem II is a multisubunit membrane complex which performs the water oxidation process in the higher plants. Core dimers and monomers of photosystem II have been isolated from thylakoid membranes by sucrose density gradient centrifugation. Lipids extracted from different photosystem II-enriched fractions obtained from spinach thylakoids have been analysed by thin layer chromatography. Cardiolipin is enriched throughout the purification of photosystem II complexes; in particular dimers contained two times more cardiolipin than their monomeric counterparts.
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Dipartimento di Chimica, Università di Bari, via Orabona 4, I-70126 Bari, Italy.
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Mallardi
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Agostiano
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D002308:Cardiolipins / Q000737:chemistry* / Q000302:isolation & purification*; D005591:Chemical Fractionation / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods*; D045332:Photosystem II Protein Complex / Q000737:chemistry* / Q000302:isolation & purification*; D018515:Plant Leaves / Q000378:metabolism*; D018724:Spinacia oleracea / Q000378:metabolism; D020524:Thylakoids / Q000378:metabolism*
D002308:Cardiolipins; D045332:Photosystem II Protein Complex