title: Identification of a Shiga-toxin type I variant containing an IS1203-like element, from Shiga-toxin producing Escherichia coli O157:H7.
abstract: We found two Shiga toxin producing Escherichia coli O157:H7 strains isolated from humans carrying the stx(1) gene with an IS1203-like element (designated as IS1203v(1)). The IS1203v(1) was inserted into the coding region of the A subunit 7 bp upstream from the TGA termination codon, resulting in a loss of two amino acid residues (Ser-Ser) from its C terminus. Toxicity of the Stx1 was confirmed by Vero cell assay. IS1203v(1) hardly affected the stx(1) gene in either its expression or the toxicity of its product.
authors.affiliation: Aichi Prefectural Institute of Public Health, 7-6 Nagare, Tsuji-machi, Kita-ku, Nagoya 462-8576, Japan. masahiro_4_suzuki@pref.aichi.lg.jp
authors.forename: Masahiro
authors.initials: M
authors.lastname: Suzuki
authors.forename: Fumio
authors.initials: F
authors.lastname: Kondo
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ito
authors.forename: Masakado
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Mami
authors.initials: M
authors.lastname: Hata
authors.forename: Hisao
authors.initials: H
authors.lastname: Oka
authors.forename: Masao
authors.initials: M
authors.lastname: Takahashi
authors.forename: Kenji
authors.initials: K
authors.lastname: Sakae
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D002851:Chromatography, High Pressure Liquid; D018388:Codon, Terminator; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D019453:Escherichia coli O157 / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D021122:Protein Subunits / Q000235:genetics; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D022622:Shiga Toxin 1 / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000633:toxicity; D014709:Vero Cells
chemical_list: D018388:Codon, Terminator; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D021122:Protein Subunits; D022622:Shiga Toxin 1