Heritable transgene expression pattern imposed onto maize ubiquitin promoter by maize adh-1 matrix attachment regions: tissue and developmental specificity in maize transgenic plants.
Matrix attachment regions (MARs) have been used to enhance transgene expression and to reduce transgene expression instability in various organisms. In plants, contradictory data question the role of MAR sequences. To assess the use of MAR sequences in maize, we have used two well-characterized MARs from the maize adh-1 region. The MARs have been cloned either 5' to or at both sides of a reporter gene expression cassette to reconstitute a MAR-based domain. Histochemical staining revealed a new transgene expression pattern in roots of regenerated plants and their progeny. Furthermore, MARs systematically induced variegation. We show here that maize adh-1 MARs are able to modify transgene expression patterns as a heritable trait, giving a new and complementary outcome following use of MARs in genetic transformation.
Torney
François
F
UMR INRA-UBP 1095 Amélioration et Santé des Plantes, Domaine de Crouelle, 63039, Clermont-Ferrand, France.
Partier
Anne
A
Says-Lesage
Véronique
V
Nadaud
Isabelle
I
Barret
Pierre
P
Beckert
Michel
M
D000444:Aldehyde Dehydrogenase / Q000235:genetics*; D000080924:Aldehyde Dehydrogenase 1 Family; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D017931:DNA Primers; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics*; D045170:Matrix Attachment Regions / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D050697:Retinal Dehydrogenase; D025801:Ubiquitin / Q000235:genetics*; D003313:Zea mays / Q000235:genetics* / Q000254:growth & development
D017931:DNA Primers; D007527:Isoenzymes; D025801:Ubiquitin; D000080924:Aldehyde Dehydrogenase 1 Family; D000444:Aldehyde Dehydrogenase; D050697:Retinal Dehydrogenase; D005966:Glucuronidase