Mammary lesions associated with bovine herpesvirus type 4 in a cow with clinical mastitis.
Intranuclear eosinophilic inclusion bodies were seen in the lactiferous duct and sinus epithelium of mammary tissues collected from a cow with clinical mastitis. Transmission electron microscopy revealed herpesvirus particles in these cells. Immunolabeling against anti bovine herpesvirus type 4 (BHV-4) rabbit serum was detected in nuclei that had intranuclear inclusion bodies. In addition, BHV-4 was isolated from the mammary tissue. The viral DNA was detected by nested PCR from the same tissue. This is the first report to describe mammary lesions in association with BHV-4.
Miyano
Hideki
H
Fukushima Prefectural Kentyu Livestock Hygiene Service Center, Koriyama, Japan.
Haritani
Makoto
M
Sentsui
Hiroshi
H
Tsuboi
Takamitsu
T
Tanimura
Nobuhiko
N
Kimura
Kumiko M
KM
Kobayashi
Masaru
M
Obara
Naoki
N
Akimoto
Yuzuru
Y
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000473:pathology* / Q000821:virology*; D006566:Herpesviridae Infections / Q000150:complications / Q000473:pathology / Q000662:veterinary*; D026021:Herpesvirus 4, Bovine / Q000648:ultrastructure*; D007150:Immunohistochemistry; D007181:Inclusion Bodies, Viral / Q000473:pathology*; D008321:Mammary Glands, Animal / Q000473:pathology / Q000821:virology; D008414:Mastitis, Bovine / Q000150:complications / Q000473:pathology* / Q000821:virology; D008854:Microscopy, Electron; D016133:Polymerase Chain Reaction; D014412:Tumor Virus Infections / Q000150:complications / Q000473:pathology / Q000662:veterinary*