title: Genotoxic evaluation of a vinifera skin extract that present pharmacological activities.
abstract: Toxicity of an alcohol-free hydro-alcoholic grape skin extract (GSE) obtained from red grapes Vitis labrusca (Isabel varietal) that present antihypertensive, vasodilator and antioxidant effects was estimated by different bioassays. Using the Salmonella/microsome assay for strains TA97, TA98, TA100 and TA102 no mutagenicity was detected for all tested concentrations (0.1-100 microg/ml), even with metabolization. Nevertheless, cytotoxicity was observed for TA97 and TA102 with and without metabolization and for TA100 with metabolization. The measurement of beta-galactosidase induction in the SOS-chromotest was positive only for Escherichia coli PQ37 when metabolization enzymes were present. Using Balb/c 3T3 fibroblasts, DNA strand breaks induction by GSE was also investigated by the comet assay and no significative difference was detected for treated and no treated DNA for 60 min. Our data suggest that GSE although no mutagenic presents cytotoxic activity.
authors.affiliation: Departamento de Biofísica e Biometria e, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, 20551-030, Rio de Janeiro, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Aiub
authors.forename: L
authors.initials: L
authors.lastname: Stankevicins
authors.forename: V
authors.initials: V
authors.lastname: da Costa
authors.forename: F
authors.initials: F
authors.lastname: Ferreira
authors.forename: J
authors.initials: J
authors.lastname: Mazzei
authors.forename: A
authors.initials: A
authors.lastname: Ribeiro da Silva
authors.forename: R
authors.initials: R
authors.lastname: Soares de Moura
authors.forename: I
authors.initials: I
authors.lastname: Felzenszwalb
mesh_terms: D000818:Animals; D020552:Comet Assay; D004249:DNA Damage; D004790:Enzyme Induction; D004926:Escherichia coli / Q000235:genetics; D005347:Fibroblasts; D051379:Mice; D008807:Mice, Inbred BALB C; D009152:Mutagenicity Tests; D010936:Plant Extracts / Q000633:toxicity; D018570:Risk Assessment; D012475:Salmonella / Q000235:genetics; D027843:Vitis / Q000737:chemistry*; D014920:Wine; D001616:beta-Galactosidase / Q000096:biosynthesis
chemical_list: D010936:Plant Extracts; D001616:beta-Galactosidase