The Filamin-A is a partner of Tc-mip, a new adapter protein involved in c-maf-dependent Th2 signaling pathway.
Using a yeast two-hybrid screen, we identified Filamin-A as a binding partner of the new adapter protein c-mip (c-maf inducing protein) and it's splice variant Tc-mip (truncated c-maf inducing protein). We have previously shown that Tc-mip is involved in Th2 signaling pathway and cytoskeletal reorganization in patients with minimal change nephrotic syndrome (MCNS), the most frequent glomerular disease in children. We showed that Filamin-A and c-mip or Tc-mip co-immunoprecipitate from c-mip or Tc-mip Jurkat transfected cells using antibodies directed against both types of proteins. In co-immunoprecipitate Jurkat cells, Filamin-A and c-mip were distributed evenly in the cytoplasm, whereas in Tc-mip-transfected Jurkat cells, Filamin-A was expressed in zones facing the cell contact. Moreover, we found that Filamin-A was upregulated in T lymphocytes of MCNS patients, as compared to normal subjects. These findings suggest that Filamin-A interacts with c-mip/Tc-mip in this new T-cell signaling pathway.
Grimbert
Philippe
P
Unité INSERM 581, Hôpital Henri Mondor, AP-HP, Université Paris XII, 51, Avenue du Mal. de Lattre-de-Tassigny, 94010 Créteil, France.
Valanciute
Asta
A
Audard
Vincent
V
Lang
Philippe
P
Guellaën
Georges
G
Sahali
Djillali
D
D048868:Adaptor Proteins, Signal Transducing; D003285:Contractile Proteins / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D064448:Filamins; D006801:Humans; D019169:Jurkat Cells; D008840:Microfilament Proteins / Q000378:metabolism*; D009402:Nephrosis, Lipoid / Q000378:metabolism; D011233:Precipitin Tests; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D051257:Proto-Oncogene Proteins c-maf; D015398:Signal Transduction / Q000276:immunology; D018418:Th2 Cells / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; C540426:CMIP protein, human; D003285:Contractile Proteins; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; D064448:Filamins; C495607:MAF protein, human; D008840:Microfilament Proteins; D011518:Proto-Oncogene Proteins; D051257:Proto-Oncogene Proteins c-maf