title: Immunofluorescence assay for serologic diagnosis of SARS.
abstract: We evaluated an indirect immunofluorescence assay based on virus-infected cells for detecting anti-severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, People's Republic of China. paulkschan@cuhk.edu.hk
authors.forename: Paul K S
authors.initials: PK
authors.lastname: Chan
authors.forename: King-Cheung
authors.initials: KC
authors.lastname: Ng
authors.forename: Rickjason C W
authors.initials: RC
authors.lastname: Chan
authors.forename: Rebecca K Y
authors.initials: RK
authors.lastname: Lam
authors.forename: Viola C Y
authors.initials: VC
authors.lastname: Chow
authors.forename: Mamie
authors.initials: M
authors.lastname: Hui
authors.forename: Alan
authors.initials: A
authors.lastname: Wu
authors.forename: Nelson
authors.initials: N
authors.lastname: Lee
authors.forename: Florence H Y
authors.initials: FH
authors.lastname: Yap
authors.forename: Frankie W T
authors.initials: FW
authors.lastname: Cheng
authors.forename: Joseph J Y
authors.initials: JJ
authors.lastname: Sung
authors.forename: John S
authors.initials: JS
authors.lastname: Tam
mesh_terms: D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D021821:Communicable Diseases, Emerging / Q000175:diagnosis* / Q000276:immunology / Q000503:physiopathology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D006723:Hong Kong; D006801:Humans; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045169:Severe Acute Respiratory Syndrome / Q000175:diagnosis* / Q000276:immunology / Q000503:physiopathology
chemical_list: D000914:Antibodies, Viral