title: Multipotential osteosarcoma with various mesenchymal differentiations in a young dog.
abstract: Apparently synchronous, aggressive, mixed mesenchymal tumors in the right tibia, right femur, left femur, and rib cage produced multiple microscopic metastases in the lungs and macroscopic metastases in the liver, kidney, and spleen in a 1.5-year-old, neutered male, mixed-breed dog. No primary soft tissue tumor mass was present. Microscopically, the neoplasm exhibited osteosarcomatous, chondrosarcomatous, liposarcomatous, leiomyosarcomatous, fibrosarcomatous, angiosarcomatous, and leukocytic differentiation and was diagnosed as a multipotential osteosarcoma with various mesenchymal differentiation. Immunohistochemically, the neoplasm was cytoplasmically immunoreactive for vimentin, osteonectin, osteocalcin, CD 18, CD 31, desmin, and muscle-specific actin. Oil Red O staining was positive within liposarcomatous areas. Skeletal metastases from a primary bone tumor are exceedingly rare in human and veterinary medicine. However, the history, clinical signs, location, microscopic and immunohistochemical features were similar to those described in aggressive, poorly differentiated osteosarcomas of children. In addition, the wide range of mesenchymal tissue differentiation of this neoplasm was unusual, and to the authors' knowledge, an osteosarcoma with this degree of multiple differentiation has not been previously reported in the dog.
authors.affiliation: Department of Pathobiology and Diagnostic Investigation, Diagnostic Center for Population and Animal Health, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48823, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Hoenerhoff
authors.forename: M
authors.initials: M
authors.lastname: Kiupel
authors.forename: D
authors.initials: D
authors.lastname: Rosenstein
authors.forename: R R
authors.initials: RR
authors.lastname: Pool
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000473:pathology / Q000662:veterinary*; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000556:secondary / Q000662:veterinary; D008113:Liver Neoplasms / Q000556:secondary / Q000662:veterinary; D008297:Male; D009361:Neoplasm Invasiveness; D012516:Osteosarcoma / Q000473:pathology / Q000662:veterinary*; D013160:Splenic Neoplasms / Q000556:secondary / Q000662:veterinary