title: [Expression of PTEN protein in oromaxillofacial tumor cell lines].
abstract: OBJECTIVE
  To investigate expression of PTEN protein in oromaxillofacial tumor cell lines and to compare the difference of PTEN protein expression between highly metastatic cancer cell lines and their parent cell lines.
  
  
  METHODS
  ABC immunohistochemical staining was applied for detecting expression of PTEN protein in 8 kinds of cancer cell lines, including tongue cancer cell lines Tca8113 and HSC-3,oral bottom cancer cell line HSC-2, buccal cancer cell line BcaCD885, mucoepidermoid carcinoma cell line MEC-1, adenoid cystic carcinoma cell line SACC83, highly metastatic tongue cancer cell line Tb-TLP and highly metastatic mucoepidermoid carcinoma cell line M3SP2.
  
  
  RESULTS
  Of the 8 kinds of cancer cell lines, 5 kinds of cancer cell lines were positive for PTEN protein expression. PTEN protein was located in cytoplasm around nucleus. 3 kinds of cancer cell lines, BcaCD885, Tb-TLP and M3SP2, lacked expression of PTEN protein.
  
  
  CONCLUSIONS
  Deficiency of PTEN protein expression may play a certain role in cancer cell metastasis.
authors.affiliation: Department of Oral Biology, College of Stomatology, Fourth Military Medical University, Xi'an 710032, China. kqyljd@fmmu.edu.cn
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Jun-zheng
authors.initials: JZ
authors.lastname: Wu
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Duan
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D003528:Carcinoma, Adenoid Cystic / Q000201:enzymology / Q000235:genetics; D018277:Carcinoma, Mucoepidermoid / Q000201:enzymology / Q000235:genetics; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000201:enzymology* / Q000235:genetics; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism*; D014062:Tongue Neoplasms / Q000201:enzymology* / Q000235:genetics
chemical_list: D051059:PTEN Phosphohydrolase