[Mechanism of charge separation and their stabilization in bacterial reaction centers].
The nuclear wavepacket formed by 20-fs excitation on the P* potential energy surface in native and mutant (YM210W and YM210L) reaction centers of Rhodobacter (Rb.) sphaeroides and Chloroflexus (C.) aurantiacus RCs was found to be reversibly transferred to the P+BA- surface at 120, 380, and 640-fs delays (monitored by measurements of BA- absorption at 1020-1028 nm). The reaction centers of YM210W(L) mutant show the most simple pattern of fs oscillations with a period of 230 fs in stimulated emission from P* and in the product P+BA-. The mechanisms of the electron transfer pathway between P* and BA and of the stabilization of the state P+BA- in bacterial reaction centers are discussed.
Iakovlev
A G
AG
Department of Photobiophysics, Belozerskii Research Institute, Moscow State University, Vorob'evy Gory, 119992 Russia.
Vasil'eva
L G
LG
Shkuropatov
A Ia
AIa
Bolgarina
T I
TI
Shkuropatova
V A
VA
Dolgova
T A
TA
Shuvalov
V A
VA
D041861:Chloroflexus / Q000737:chemistry; D004579:Electron Transport; D007700:Kinetics; D009154:Mutation; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012242:Rhodobacter sphaeroides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013053:Spectrophotometry / Q000379:methods; D014443:Tyrosine / Q000737:chemistry / Q000235:genetics
D045322:Photosynthetic Reaction Center Complex Proteins; D014443:Tyrosine