Comparison of flow cytometric and Alamar Blue tests with the proportional method for testing susceptibility of Mycobacterium tuberculosis to rifampin and isoniazid.
The performance of flow cytometry and the microplate Alamar Blue assay in determining susceptibility of Mycobacterium tuberculosis was assessed by testing 150 Brazilian isolates. The overall agreement was 97.3 and 98% for isoniazid and 94.7 and 100% for rifampin by flow cytometry and MABA, respectively. This study was entirely done in a developing country.
Reis
Roberto S
RS
Oswaldo Cruz Fundation (FIOCRUZ), Rio de Janeiro, Brazil.
Neves
Ivan
I
Lourenço
Sergio L S
SL
Fonseca
Leila S
LS
Lourenço
Maria Cristina S
MC
D000995:Antitubercular Agents / Q000494:pharmacology*; D001938:Brazil; D004396:Coloring Agents; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000706:statistics & numerical data; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification; D010078:Oxazines; D012293:Rifampin / Q000494:pharmacology*; D014966:Xanthenes
D000995:Antitubercular Agents; D004396:Coloring Agents; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin; D007538:Isoniazid; D012293:Rifampin