[Modification of the chemiluminescent analysis method for evaluation of the whole blood phagocyte activity in sensibilized animals].
A modified method of chemiluminescence analysis was developed in experiments with animals. Whole blood of guinea pigs was made use of as the biological sample. BaSO4 and another new substance, i.e. aerosol, were used in the phagocytes' stimulation of whole blood. Optimal concentrations of reaction components, the incubation period of whole blood samples and of specific allergens and the duration of chemiluminescence registration were defined. The discussed modified method of chemiluminescence analysis can be used to monitor the specific treatment schemes by immunotherapy and to choose adequate schemes for the mentioned therapy.
Mitereva
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DE
Agafonov
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VE
D000485:Allergens / Q000276:immunology; D000818:Animals; D006168:Guinea Pigs; D006967:Hypersensitivity / Q000276:immunology*; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D009504:Neutrophils / Q000737:chemistry / Q000276:immunology; D010586:Phagocytes / Q000737:chemistry / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology
D000485:Allergens; D012710:Serum Albumin, Bovine