Preparation of protein gradients through the controlled deposition of protein-nanoparticle conjugates onto functionalized surfaces.
This paper describes a simple method for the preparation and characterization of protein density gradients on solid supports. The method employs colloidal metal nanoparticles as protein carriers and optical tags and is capable of forming linear, exponential, 1D, 2D, and multiprotein gradients of varying slope without expensive or sophisticated surface patterning techniques. Surfaces patterned with proteins using the procedures described within are shown to support cell growth and are thus suitable for studies of protein-cell interactions.
Krämer
Stephan
S
Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA.
Xie
Huan
H
Gaff
John
J
Williamson
John R
JR
Tkachenko
Alexander G
AG
Nouri
Navid
N
Feldheim
David A
DA
Feldheim
Daniel L
DL
D000818:Animals; D001369:Axons / Q000187:drug effects / Q000502:physiology; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D017979:Gold Colloid / Q000737:chemistry / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology; D008670:Metals / Q000737:chemistry; D008856:Microscopy, Fluorescence; D036103:Nanotechnology; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D011107:Polylysine / Q000737:chemistry; D051381:Rats; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000494:pharmacology*; D013053:Spectrophotometry
D017979:Gold Colloid; D008670:Metals; D011107:Polylysine; D012710:Serum Albumin, Bovine