Preoperative endoscopic analysis of thymidine phosphorylase and dihydropyrimidine dehydrogenase in gastrointestinal cancer.
Thymidine phosphorylase (TP) and dihydropyrimidine dehydrogenase (DPD) are considered to be key enzymes affecting the prognosis for patients with advanced gastrointestinal cancer. Preoperative examination of TP and DPD expression levels and assessment of these enzymes in inoperable cancer patients may contribute to successful treatment. We tried to prove the correlation of TP and DPD expression in preoperative specimens by endoscopy and in surgical specimens. The present study was designed to quantify TP and DPD levels by enzyme-linked immunosorbent assay (ELISA) in tumor tissue obtained from 30 gastrointestinal cancer patients by preoperative endoscopy and surgery, including 15 gastric and 15 colorectal cancers. Successful cases as those in which cancer cells were demonstrated histologically in preoperative specimens by endoscopy were 12 (success rate: 80%) in gastric cancer patients, and 15 (success rate: 100%) in colorectal cancer patients. In successful cases, there were almost significant correlations in all cases, gastric cancer, and colorectal cancer among the expression of TP, DPD, and TP/DPD ratio in each preoperative specimen by endoscopy and surgical specimen, respectively. On the other hand, in the gastric cancer group, 3 unsuccessful cases resulted in a significant departure from ideal equation compared with 12 successful cases. In actual clinical care, physicians should pay attention to and evaluate carefully the data from endoscopical biopsy specimens in which cancer cells may not be demonstrated histologically. Thus, endoscopic analysis of TP and DPD expression in preoperative or inoperable cancer patients may contribute to successful treatment.
Hotta
Tsukasa
T
Department of Surgery, Wakayama Rosai Hospital, Wakayama 640-8505, Japan. hotta@naxnet.or.jp
Taniguchi
Katsutoshi
K
Kobayashi
Yasuhito
Y
Johata
Kiyofumi
K
Sahara
Masaki
M
Naka
Teiji
T
Watanabe
Takashi
T
Ochiai
Minoru
M
Tanimura
Hiroshi
H
Tsubota
Yukari T
YT
D000368:Aged; D001808:Blood Vessels / Q000473:pathology; D042943:Dihydrouracil Dehydrogenase (NADP) / Q000378:metabolism*; D004724:Endoscopy; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000201:enzymology* / Q000473:pathology / Q000601:surgery; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011300:Preoperative Care; D013939:Thymidine Phosphorylase / Q000378:metabolism*
D042943:Dihydrouracil Dehydrogenase (NADP); D013939:Thymidine Phosphorylase