[Expression of PTEN protein in oromaxillofacial tumor cell lines].
OBJECTIVE
To investigate expression of PTEN protein in oromaxillofacial tumor cell lines and to compare the difference of PTEN protein expression between highly metastatic cancer cell lines and their parent cell lines.


METHODS
ABC immunohistochemical staining was applied for detecting expression of PTEN protein in 8 kinds of cancer cell lines, including tongue cancer cell lines Tca8113 and HSC-3,oral bottom cancer cell line HSC-2, buccal cancer cell line BcaCD885, mucoepidermoid carcinoma cell line MEC-1, adenoid cystic carcinoma cell line SACC83, highly metastatic tongue cancer cell line Tb-TLP and highly metastatic mucoepidermoid carcinoma cell line M3SP2.


RESULTS
Of the 8 kinds of cancer cell lines, 5 kinds of cancer cell lines were positive for PTEN protein expression. PTEN protein was located in cytoplasm around nucleus. 3 kinds of cancer cell lines, BcaCD885, Tb-TLP and M3SP2, lacked expression of PTEN protein.


CONCLUSIONS
Deficiency of PTEN protein expression may play a certain role in cancer cell metastasis.
Liu
Bin
B
Department of Oral Biology, College of Stomatology, Fourth Military Medical University, Xi'an 710032, China. kqyljd@fmmu.edu.cn
Wu
Jun-zheng
JZ
Duan
Xiao-hong
XH
Li
Jie
J
Li
Yan
Y
D000818:Animals; D003528:Carcinoma, Adenoid Cystic / Q000201:enzymology / Q000235:genetics; D018277:Carcinoma, Mucoepidermoid / Q000201:enzymology / Q000235:genetics; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000201:enzymology* / Q000235:genetics; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism*; D014062:Tongue Neoplasms / Q000201:enzymology* / Q000235:genetics
D051059:PTEN Phosphohydrolase