Identification of an Arabidopsis inorganic pyrophosphatase capable of being imported into chloroplasts.
An Arabidopsis cDNA coding for a previously uncharacterized isoform of inorganic pyrophosphatase was isolated. It was used to complement an E. coli mutant, demonstrating that it coded for an active enzyme. MgCl(2) was necessary for the protein's activity, whilst NaF inhibited it. The K(m) for pyrophosphate and the pH optimum of the protein was determined. The gene coding for this protein was expressed in all tissues, and its expression in rosette leaves was induced by incubation on metabolizable sugars. In vitro import experiments demonstrated that the protein could be imported into chloroplasts and localized to the stromal compartment.
Schulze
Silke
S
Max-Planck-Institute of Molecular Plant Physiology, Golm, Germany.
Mant
Alexandra
A
Kossmann
Jens
J
Lloyd
James R
JR
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D002736:Chloroplasts / Q000201:enzymology* / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005816:Genetic Complementation Test; D006863:Hydrogen-Ion Concentration; D043564:Inorganic Pyrophosphatase / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D015636:Magnesium Chloride / Q000494:pharmacology; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000201:enzymology; D010802:Phylogeny; D018087:Plastids / Q000378:metabolism; D020033:Protein Isoforms; D021381:Protein Transport; D012313:RNA / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012969:Sodium Fluoride / Q000494:pharmacology; D014018:Tissue Distribution
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D020033:Protein Isoforms; D012333:RNA, Messenger; D015636:Magnesium Chloride; D012313:RNA; D012969:Sodium Fluoride; D043564:Inorganic Pyrophosphatase; C487103:PPa6 protein, Arabidopsis