High-throughput preparative process utilizing three complementary chromatographic purification technologies.
A high-throughput process was developed in which wells in plates generated from parallel synthesis are automatically channeled to an appropriate purification technique using analytical data as a guide. Samples are directed to either of three fundamentally different preparative techniques: HPLC with UV-triggered fraction collection, supercritical fluid chromatography (SFC) with UV-triggered fraction collection, or HPLC with MS-triggered fraction collection. Automated analysis of the analytical data identifies the product compound mass and creates work lists based on chromatographic properties exhibited in the data so that each preparative instrument cherry picks the appropriate list of samples to purify when a preparative-scale plate is loaded.
Ventura
Manuel
M
Pfizer Global R&D-La Jolla, 10770 Science Center Drive, San Diego, CA 92121, USA. manuel.ventura@pfizer.com
Farrell
William
W
Aurigemma
Christine
C
Tivel
Kathleen
K
Greig
Michael
M
Wheatley
Jeffrey
J
Yanovsky
Alex
A
Milgram
Kenneth Eric
KE
Dalesandro
David
D
DeGuzman
Raylyn
R
Tran
Phuong
P
Nguyen
Leslie
L
Chung
Loanne
L
Gron
Ole
O
Koch
Charles A
CA
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet