Improved skin flap survival after local heat preconditioning in pigs.
BACKGROUND
Preconditioning induces the expression of heat shock proteins (HSPs), which can help a cell survive an acute episode of stress. Similar to the induction of HSP expression, the cell protection is independent of the type of stress. The aim of this study was to test in a large, randomized animal model, if skin flap survival may be improved by local heat preconditioning and induction of HSP 70.


METHODS
Twenty-four hours before surgery, a heating blanket was laid on the buttocks of large white pigs. In the preconditioned group (n = 6), the blanket was warmed up to 43 degrees C for 3 x 30 min, whereas it was kept at room temperature in between the heating episodes as well as in the control animals (n = 6). A random pattern skin flap was raised on both sides of the buttocks. Flap survival was measured clinically. Induction of HSP and apoptosis were assessed quantitatively by immunohistochemistry and TUNEL assay, respectively.


RESULTS
Preconditioning reduced flap necrosis from 40 +/- 8% of the total flap surface to 7 +/- 14% (P < 0.01). Induction of HSP was significantly higher in the experimental group (79 +/- 12% versus 42 +/- 13%, P < 0.01), whereas apoptosis in healthy flap tissue was reduced from 30 +/- 11 to 11 +/- 6 cells/visual field (P < 0.01).


CONCLUSIONS
In the present study, necrosis and apoptosis rate of skin flaps could be reduced significantly due to local heat preconditioning. Our results suggest that ischemia-related wound healing complications could be diminished with local heat application, a most simple and least invasive method of preconditioning.
Harder
Y
Y
Division of Plastic, Reconstructive, and Aesthetic Surgery, University Hospital, CH-3010 Berne, Switzerland. yvesharder@bluewin.ch
Contaldo
C
C
Klenk
J
J
Banic
A
A
Jakob
S M
SM
Erni
D
D
D000818:Animals; D017209:Apoptosis; D003213:Conditioning, Psychological; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006358:Hot Temperature; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D009336:Necrosis; D012867:Skin / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D013524:Surgical Flaps; D013552:Swine; D014022:Tissue Survival
D018840:HSP70 Heat-Shock Proteins