Syntaxin 5 interacts with presenilin holoproteins, but not with their N- or C-terminal fragments, and affects beta-amyloid peptide production.
Mutations in presenilins 1 and 2 (PS1 and PS2) account for the majority of cases of early-onset familial Alzheimer's disease. However, the trafficking and interaction of PSs with other proteins in the early secretory pathways are poorly understood. Using co-immunoprecipitation, we found that PS bound to Syx5 (syntaxin 5), which is a target-soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor involved in endoplasmic reticulum (ER)-Golgi vesicular transport in vivo. Syx5 interacted only with the full-length PS holoproteins and not with the naturally occurring N- or C-terminal fragments. The PS holoproteins co-immunoprecipitated with the mutant Syx5, which localized to the ER and Golgi compartments, despite the substitution of the transmembrane region with that of syntaxin 1A. In contrast, the transmembrane deletion mutant that localized to the cytosol, but not to the ER or Golgi compartments, did not co-immunoprecipitate the PS holoproteins. The PS1 variant linked to familial Alzheimer's disease (PS1DeltaE9), lacking the region that contains the endoproteolytic cleavage site in the cytoplasmic loop, showed markedly decreased binding to Syx5. Immunofluorescence and sucrose-density-gradient fractionation analyses showed that the full-length PS holoproteins co-localized with Syx5 to the ER and cis-Golgi compartments. Furthermore, Syx5 overexpression resulted in the accumulation of PS holoproteins and the beta-amyloid precursor protein, and reduced the secretion of the Abeta (amyloid beta) peptide in COS-7 cells. In summary, these results indicate that Syx5 binds to full-length PSs and affects the processing and trafficking of beta-amyloid precursor protein in the early secretory compartments.
Suga
Kei
K
Department of Cell Physiology, Kyorin University School of Medicine, 6-20-2, Shinkawa, Mitaka, Tokyo 181-8611, Japan. ksuga@kyorin-u.ac.jp
Tomiyama
Takami
T
Mori
Hiroshi
H
Akagawa
Kimio
K
D016229:Amyloid beta-Peptides / Q000096:biosynthesis*; D000818:Animals; D019556:COS Cells / Q000737:chemistry / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D006367:HeLa Cells / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D009154:Mutation / Q000235:genetics; D010452:Peptide Biosynthesis / Q000502:physiology*; D010455:Peptides / Q000378:metabolism*; D053764:Presenilin-1; D053766:Presenilin-2; D025941:Protein Interaction Mapping / Q000379:methods; D017434:Protein Structure, Tertiary; D050765:Qa-SNARE Proteins; D000685:Serum Amyloid A Protein / Q000096:biosynthesis; D050827:Syntaxin 1; D014162:Transfection
D016229:Amyloid beta-Peptides; D008565:Membrane Proteins; C508853:PSEN1 protein, human; C508861:PSEN2 protein, human; D010455:Peptides; D053764:Presenilin-1; D053766:Presenilin-2; D050765:Qa-SNARE Proteins; C494212:STX1A protein, human; D000685:Serum Amyloid A Protein; D050827:Syntaxin 1