title: Effect of 2'-deoxyisoguanosine on the growth of human tumor and normal cell lines.
abstract: OBJECTIVE
  To test the effect of 2'-deoxyisoguanosine as a specific substrate of HIV reverse transcriptase on the growth of cultured normal and cancer cells.
  
  
  METHODS
  The effect of 2'-deoxyisoguanosine on the growth of cells of two normal (telomerase-negative) and five cancer cell lines have been tested. Cell viability was assessed by MTT assay.
  
  
  RESULTS
  We have found that this nucleoside analogue has low potency and specificity in inhibiting tumor cell growth. IC50 ranged from 0.5 mM to 2 mM for tumor cells, and from 1 mM to more than 4 mM for normal cells.
  
  
  CONCLUSIONS
  2'-deoxyisoguanosine has low potency and specificity in inhibiting tumor cell growth, similar to other telomerase inhibitors.
authors.affiliation: Department of Molecular Biology, Maria Sklodowska-Curie Memorial Cancer Center, Institute of Oncology, Warsaw, Poland. radka@coi.waw.pl
authors.forename: Radoslawa
authors.initials: R
authors.lastname: Nowak
authors.forename: Maria
authors.initials: M
authors.lastname: Sawardo-Rochowska
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Kazimierczuk
authors.forename: Roman J
authors.initials: RJ
authors.lastname: Nowak
mesh_terms: D000241:Adenosine; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D006151:Guanosine / Q000494:pharmacology*; D006801:Humans; D020128:Inhibitory Concentration 50; D013379:Substrate Specificity; D019098:Telomerase / Q000378:metabolism
chemical_list: D006151:Guanosine; C008184:isoguanosine; D019098:Telomerase; D000241:Adenosine