The effect of growth medium salinity of Photobacterium damselae subsp. piscicida on the immune response of hybrid bass (Morone saxatilis x M. chrysops).
Photobacterium damselae subsp. piscicida (P. damselae) was grown on various media and the effect of media salinity on certain immune responses of hybrid bass was studied. In Israel, pasteurellosis outbreaks have not been reported at water salinities below 1.38 per thousand. During vaccination experiments the salinity of the medium on which P. damselae is grown, was shown to affect stimulation of the immune system. No correlation was found between antibody response and protection. Bacterial envelopes separated by electrophoresis and subjected to western blot analysis revealed an antibody response against some protein bands. Band sequencing was performed to identify the protein stimulating the immune response. Sequence identity of 80% was seen in 10-amino-acid overlap of the 36-kDa band with a specific gene of alkalophilic Bacillus firmus. A preparation of P. damselae grown in a 2.5% NaCl medium at 25 degrees C is the most effective vaccine against pasteurellosis, providing hybrid bass with quite good protection.
Nitzan
S
S
Central Fish Health Laboratory, Department of Fisheries and Agriculture and Rural Development, Nir-David 19150, Israel. fish_lab@netvision.net.il
Shwartsburd
B
B
Heller
E D
ED
D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001407:Bacillus / Q000737:chemistry; D001492:Bass / Q000276:immunology*; D015153:Blotting, Western; D003470:Culture Media / Q000737:chemistry; D004586:Electrophoresis; D004797:Enzyme-Linked Immunosorbent Assay; D005393:Fish Diseases / Q000276:immunology / Q000382:microbiology*; D006824:Hybridization, Genetic; D007110:Immunity, Active / Q000187:drug effects; D007557:Israel; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010326:Pasteurella Infections / Q000276:immunology / Q000662:veterinary*; D010776:Photobacterium / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology*; D014611:Vaccination / Q000662:veterinary*
D000907:Antibodies, Bacterial; D003470:Culture Media; D008565:Membrane Proteins; D012965:Sodium Chloride