title: Increased nitric oxide is accompanied by lipid oxidation in adolescent varicocele.
abstract: Summary One of the mechanisms of injury in varicocele has been proposed to be elevated nitric oxide (NO). We aimed to determine the association between the elevation of NO and lipid oxidation in varicocele compared with peripheral venous levels of these two substances as it has not been studied before. The study group consisted of 13 adolescents with left idiopathic varicocele of grades II-III. Blood specimens were obtained from dilated spermatic and peripheral veins simultaneously. Peripheral samples were also collected from 13 healthy children as controls. Nitrite/nitrate levels (NO(x)) and levels of malonedialdehyde (MDA) were determined using Griess reaction and thiobarbituric acid test, respectively. Results were compared with Kruskal-Wallis and Mann-Whitney tests. Peripheral NO(x) and MDA were the same in the study and control groups (p = 0.069 and p = 0.27, respectively). Spermatic vein NO(x) and MDA levels were elevated significantly compared with the peripheral levels in the study group (p = 0.005 and p = 0.048, respectively). Increased NO(x) levels with lipid oxidation occur locally in adolescent varicocele, implying that these events could be reversed by early treatment.
authors.affiliation: Department of Pediatric Surgery, Gazi University School of Medicine, Ankara, Turkey. zafertrk@yahoo.com
authors.forename: Zafer
authors.initials: Z
authors.lastname: Türkyilmaz
authors.forename: Sebnem
authors.initials: S
authors.lastname: Gülen
authors.forename: Kaan
authors.initials: K
authors.lastname: Sönmez
authors.forename: Ramazan
authors.initials: R
authors.lastname: Karabulut
authors.forename: Sibel
authors.initials: S
authors.lastname: Dinçer
authors.forename: A
authors.initials: A
authors.lastname: Can Başaklar
authors.forename: Nuri
authors.initials: N
authors.lastname: Kale
mesh_terms: D000293:Adolescent; D016022:Case-Control Studies; D002648:Child; D005215:Fasting / Q000097:blood; D006801:Humans; D050356:Lipid Metabolism; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism; D009566:Nitrates / Q000032:analysis / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis*; D009573:Nitrites / Q000032:analysis / Q000378:metabolism; D010084:Oxidation-Reduction; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014421:Turkey; D014646:Varicocele / Q000378:metabolism* / Q000473:pathology
chemical_list: D009566:Nitrates; D009573:Nitrites; D017392:Thiobarbituric Acid Reactive Substances; D009569:Nitric Oxide; D008315:Malondialdehyde