title: Identification of virus-encoded microRNAs.
abstract: RNA silencing processes are guided by small RNAs that are derived from double-stranded RNA. To probe for function of RNA silencing during infection of human cells by a DNA virus, we recorded the small RNA profile of cells infected by Epstein-Barr virus (EBV). We show that EBV expresses several microRNA (miRNA) genes. Given that miRNAs function in RNA silencing pathways either by targeting messenger RNAs for degradation or by repressing translation, we identified viral regulators of host and/or viral gene expression.
authors.affiliation: Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, NY 10021, USA.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Pfeffer
authors.forename: Mihaela
authors.initials: M
authors.lastname: Zavolan
authors.forename: Friedrich A
authors.initials: FA
authors.lastname: Grässer
authors.forename: Minchen
authors.initials: M
authors.lastname: Chien
authors.forename: James J
authors.initials: JJ
authors.lastname: Russo
authors.forename: Jingyue
authors.initials: J
authors.lastname: Ju
authors.forename: Bino
authors.initials: B
authors.lastname: John
authors.forename: Anton J
authors.initials: AJ
authors.lastname: Enright
authors.forename: Debora
authors.initials: D
authors.lastname: Marks
authors.forename: Chris
authors.initials: C
authors.lastname: Sander
authors.forename: Thomas
authors.initials: T
authors.lastname: Tuschl
mesh_terms: D000818:Animals; D002144:Callithrix; D002460:Cell Line; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D019295:Computational Biology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000502:physiology; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D020506:Untranslated Regions; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D017735:Virus Latency
chemical_list: C083278:BHRF1 protein, Human herpesvirus 4; D004268:DNA-Binding Proteins; D035683:MicroRNAs; D011506:Proteins; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D012367:RNA, Viral; D020506:Untranslated Regions; D014764:Viral Proteins; C083202:BALF5 protein, Epstein-barr virus; D004259:DNA-Directed DNA Polymerase