title: Thermodynamic studies on the adsorption of fibronectin adhesion-promoting peptide on nanothin films of poly(2-vinylpyridine) by SPR.
abstract: This paper describes a methodology for preparing uniform, nanothin polymer films for the study of biomolecule adsorption by surface plasmon resonance spectroscopy (SPR). The methodology combines molecular self-assembly of alkanethiols on gold with surface-confined atom transfer radical polymerization (ATRP). Poly(2-vinylpyridine) was chosen to demonstrate the methodology, and growth kinetics were studied by ex situ ellipsometry. Atomic force microscopy (AFM) indicated that the polymer films were uniform with RMS roughness of approximately 0.5 nm. Subsequent SPR measurements were done to determine thermodynamic adsorption properties (deltaG, deltaH, and deltaS) between fibronectin adhesion-promoting peptide and the surface-confined poly(2-vinylpyridine) at 15, 20, and 25 degrees C. The flexibility in synthesis conditions and the opportunities for manipulating film thicknesses and graft densities that ATRP provides to grow polymer films from gold surfaces holds advantages over conventional spin-coating and grafting to approaches in the design of model polymer films for biomolecule adsorption studies. These advantages are described.
authors.affiliation: Department of Chemical Engineering, Clemson University, Clemson, South Carolina 29634-0909, USA.
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Wei
authors.forename: Scott M
authors.initials: SM
authors.lastname: Husson
mesh_terms: D000327:Adsorption; D007700:Kinetics; D018625:Microscopy, Atomic Force; D036103:Nanotechnology; D010455:Peptides / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D020349:Surface Plasmon Resonance / Q000379:methods*; D013816:Thermodynamics
chemical_list: D010455:Peptides; D011145:Polyvinyls; C058671:poly(2-vinylpyridine)