title: The Ser(+r83k) mutation is a second site mutation of SerD affecting the N-terminus of serrate.
abstract: The Serrate gene encodes an essential ligand for Notch signaling used during development of the adult wing and other systems in Drosophila melanogaster. Animals heterozygous or homozygous for the Ser(D) allele of this gene display characteristic defects in wing margin formation. We have characterized a spontaneously arising intragenic suppressor of Ser(D) named Ser(+r83k). Homozygous double mutant Ser(+r83k), Ser(D) animals are viable, with normal wing margin formation, but display an aberrant outspread wing posture. The two mutations can be separated by meiotic recombination which restores the Ser(D) mutant phenotype and demonstrates that in the absence of Ser(D) the Ser(+r83k) mutation is homozygous lethal. These two mutations therefore display allelic compensation. Molecular analysis reveals a single C-T transition mutation within the 5' (protein encoding region) of the Ser(+r83k) transcript. This mutation is predicted to change Arginine(176) to Cysteine, possibly leading to altered interactions with the Notch receptor.
authors.affiliation: Department of Biology, Trinity College, Life Sciences Center, Hartford, Connecticut 06106, USA.
authors.forename: Tung T
authors.initials: TT
authors.lastname: Trang
authors.forename: Vera
authors.initials: V
authors.lastname: Tannous
authors.forename: Yi
authors.initials: Y
authors.lastname: Gu
authors.forename: Jessica
authors.initials: J
authors.lastname: Mosher
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Fleming
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins; D017931:DNA Primers; D029721:Drosophila Proteins; D004331:Drosophila melanogaster; D005260:Female; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D017386:Sequence Homology, Amino Acid; D012694:Serine / Q000235:genetics*; D000072099:Serrate-Jagged Proteins
chemical_list: D002135:Calcium-Binding Proteins; D017931:DNA Primers; D029721:Drosophila Proteins; D036341:Intercellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; C000606278:Ser protein, Drosophila; D000072099:Serrate-Jagged Proteins; D012694:Serine