Development and evaluation of a new commercial test allowing the simultaneous detection of noroviruses and sapoviruses by reverse transcription-PCR and microplate hybridization.
This work describes the design and initial evaluation of a commercial test allowing the detection of noroviruses and sapoviruses by reverse transcription-PCR (RT-PCR) in a single tube followed by microplate hybridization, as well as the detection of PCR inhibitors. The test was shown to be broadly reactive (except for Melksham-like strains), sensitive, and specific and thus should be useful for calicivirus detection in clinical practice.
Bon
F
F
Laboratoire de Virologie, Reference Laboratory for Enteric Viruses, Centre Hospitalier Universitaire, and Microbiologie Médicale et Moléculaire, UFRS Médecine et Pharmacie, Dijon, France.
Giraudon
H
H
Sancey
C
C
Barranger
C
C
Joannes
M
M
Pothier
P
P
Kohli
E
E
D017250:Caliciviridae Infections / Q000453:epidemiology / Q000821:virology; D004196:Disease Outbreaks; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology; D006801:Humans; D029322:Norovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D029341:Sapovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods* / Q000706:statistics & numerical data