Ultrahigh resolution optical coherence tomography of the monkey fovea. Identification of retinal sublayers by correlation with semithin histology sections.
Optical coherence tomography (OCT) has become an established diagnostic tool for the clinical assessment of retinal pathology but correlation of acquired signals with retinal substructures has often been ambiguous. In the monkey retina we have now obtained ultrahigh resolution (UHR) OCT images with 1.4 microm axial x 3 microm transverse resolution from perfusion-fixed eye cups of Macaca fascicularis and optimized the identification of retinal anatomy by correction of spatial artefacts in correlated histology. After resin embedding, serial semithin sections were obtained that corresponded to OCT transects. The direct overlay of features identified in histological sections with corresponding OCT locations was limited by non-linear tissue shrinkage due to dehydration and sectioning stress. In the present study, these misalignments were further corrected by using polygonal spline morphing based on corresponding unequivocal landmarks. The geometric normalization then allowed detailed comparison of both profiles including delicate sublayers of photoreceptor inner- and outer segments. Such correlation will facilitate the extraction of structural information from in vivo ultrahigh resolution OCT images in clinical and experimental applications.
Anger
Elisabeth M
EM
Department of Physiology, Medical University of Vienna, Vienna, Austria.
Unterhuber
Angelika
A
Hermann
Boris
B
Sattmann
Harald
H
Schubert
Christian
C
Morgan
James E
JE
Cowey
Alan
A
Ahnelt
Peter K
PK
Drexler
Wolfgang
W
D000818:Animals; D016477:Artifacts; D005584:Fovea Centralis / Q000648:ultrastructure*; D008252:Macaca fascicularis / Q000033:anatomy & histology*; D009898:Optic Disk / Q000648:ultrastructure; D020419:Photoreceptor Cells, Vertebrate / Q000648:ultrastructure; D012160:Retina / Q000648:ultrastructure; D041623:Tomography, Optical Coherence / Q000379:methods