It's green outside: tracking cell surface proteins with pH-sensitive GFP.
Green fluorescent protein (GFP) and mutated GFP variants have proved to be immensely powerful tools that have had a profound impact on research in biological sciences. This review considers the development, use and future implications of pH-dependent GFP variants (e.g. pHluorins). These proteins hold considerable promise for the relatively non-invasive monitoring of events such as exocytosis, endocytosis and protein surface expression in living neurons with high spatial and temporal resolution.
Ashby
Michael C
MC
MRC Centre for Synaptic Plasticity, Department of Anatomy, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK.
Ibaraki
Kyoko
K
Henley
Jeremy M
JM
D000818:Animals; D002468:Cell Physiological Phenomena; D049452:Green Fluorescent Proteins; D006863:Hydrogen-Ion Concentration; D008164:Luminescent Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D009154:Mutation; D021381:Protein Transport / Q000502:physiology
D008164:Luminescent Proteins; D008565:Membrane Proteins; D049452:Green Fluorescent Proteins