Supported synthesis of ferrocene modified oligonucleotides as new electroactive DNA probes.
The use of 1-[3-O-(2-cyanoethyl-N,N-diisopropylphosphor amidityl)propyl]ferrocene and 1-[3-O-dimethoxytrityl propyl]-1'-[3'-O-(2-cyanoethyl-N,N-diisopropylphosphoramidityl) propyl] ferrocene as reactive synthons for DNA/RNA synthesizer allows to generate ferrocene-labelled oligonucleotides with remarkable DNA detection properties.
Navarro
Aude-Emmanuelle
AE
Groupe de Chimie Organique et Matériaux Moléculaires, UMR CNRS 6114, Faculté des Sciences de Luminy, case 901, 163 avenue de Luminy, 13288 Marseille Cedex 9, France.
Spinelli
Nicolas
N
Chaix
Carole
C
Moustrou
Corinne
C
Mandrand
Bernard
B
Brisset
Hugues
H
D000595:Amino Acid Sequence; D015342:DNA Probes / Q000138:chemical synthesis*; D004563:Electrochemistry; D005296:Ferrous Compounds / Q000737:chemistry*; D000075163:Metallocenes; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds
D015342:DNA Probes; D005296:Ferrous Compounds; D000075163:Metallocenes; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; C004998:ferrocene