title: Postmortem effect of pentobarbital anesthetic on survival of adult cortical neurons in primary culture.
abstract: We determined whether pentobarbital anesthetic is required to culture postmortem adult rat neurons. Pentobarbital treatment resulted in two-fold increases in neuron survival in culture after 2 and 4 h postmortem compared to non-anesthetic controls, but was not as effective as simple postmortem treatment on ice and therefore not essential.
authors.affiliation: Department of Medical Microbiology and Immunology, School of Medicine, Southern Illinois University, Springfield, IL 62794-9626, USA.
authors.forename: John J
authors.initials: JJ
authors.lastname: Viel
authors.forename: Dennis Q
authors.initials: DQ
authors.lastname: McManus
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Brewer
mesh_terms: D000759:Adjuvants, Anesthesia / Q000494:pharmacology; D000818:Animals; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D002540:Cerebral Cortex / Q000166:cytology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007053:Ice; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D010424:Pentobarbital / Q000494:pharmacology*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D011180:Postmortem Changes; D051381:Rats; D013997:Time Factors
chemical_list: D000759:Adjuvants, Anesthesia; D005904:Glial Fibrillary Acidic Protein; D007053:Ice; D010751:Phosphopyruvate Hydratase; D010424:Pentobarbital