Identification and characterisation of the fur genes in Photobacterium damselae ssp. piscicida and ssp. damselae.
The gene encoding the ferric uptake regulator protein (fur gene) of the fish pathogenic bacterium Photobacterium damselae ssp. piscicida Strain D121 was partially amplified using degenerate oligonucleotides. Complete sequencing of the fur gene and neighbouring DNA was accomplished by primer walking. An open reading frame of 447 bp, coding for a protein of 148 amino acids, and with high homology to previously described Fur proteins, was identified. The fur gene of P. damselae ssp. damselae ATCC 35083 was subsequently amplified by PCR with specific primers and its sequence determined, showing a 99.3% similarity to the P. damselae ssp. piscicida fur gene. The P. damselae fur gene was able to complement the fur mutation of Escherichia coli Strain H1681 in an iron-dependent fashion.
Juiz-Río
Sandra
S
Departamento de Microbiología y Parasitología, Instituto de Acuicultura, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Osorio
Carlos R
CR
Lemos
Manuel L
ML
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D016000:Cluster Analysis; D017931:DNA Primers; D008969:Molecular Sequence Data; D010776:Photobacterium / Q000235:genetics*; D010802:Phylogeny; D012097:Repressor Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D001426:Bacterial Proteins; D017931:DNA Primers; D012097:Repressor Proteins; C068477:ferric uptake regulating proteins, bacterial