title: Correlation of morphological findings with functional reserve in the aging donor: role of the poly (ADP-ribose) polymerase.
abstract: BACKGROUND
  The enzyme poly (ADP-ribose) polymerase (PARP-1) participates in the first events of DNA repair in higher organisms. Under conditions of tissue ischemia, this action can lead to significant decreases in NAD(+), massive adenosine triphosphate (ATP) depletion, and cell death. In renal grafts with pretransplantation cold ischemia and subsequent ischemia-reperfusion injury, overactivation of PARP-1 may lead to a higher index of acute tubular necrosis, a delay in total recovery of the function of the transplanted organ, and an early progression to chronic graft nephropathy. The present study examined whether increased tubular expression of PARP-1 in kidneys from aged donors contributed to recipient renal function.
  
  
  METHODS
  We studied the nuclear expression of PARP-1 using immunohistochemistry with monoclonal antibody PAR01 in 75 kidney biopsy specimens from 40 aged donors.
  
  
  RESULTS
  Immunohistochemical expression of PARP-1 showed a statistically significant relationship with donor age (r =.408, P =.006, Spearman test), with time required to achieve effective diuresis (r =.386, P =.01, Spearman test) and with creatinine levels in the first 3 months. We also highlighted a greater intensity of PARP-1 expression in suboptimal donor kidneys that failed to reduce the serum creatinine levels to <1.7 mg/dL (creatinine <1.7 PARP: 1.29 +/- 1.49 vs creatinine >1.7 PARP: 2.29 +/- 1.33, P =.047, Mann-Whitney U test).
  
  
  CONCLUSIONS
  We conclude that the determination of PARP-1 in biopsy specimens from aged donors may be a useful predictive factor for renal graft function.
authors.affiliation: Department of Pathology, San Cecilio University Hospital, Granada, Spain.
authors.forename: F
authors.initials: F
authors.lastname: O'Valle
authors.forename: M C
authors.initials: MC
authors.lastname: Benitez
authors.forename: M
authors.initials: M
authors.lastname: Gómez-Morales
authors.forename: J
authors.initials: J
authors.lastname: Bravo
authors.forename: A
authors.initials: A
authors.lastname: Osuna
authors.forename: R M G
authors.initials: RM
authors.lastname: del Moral
authors.forename: D
authors.initials: D
authors.lastname: Martin-Oliva
authors.forename: F J
authors.initials: FJ
authors.lastname: Oliver
authors.forename: R G
authors.initials: RG
authors.lastname: del Moral
mesh_terms: D000368:Aged; D001331:Automation; D001706:Biopsy; D006801:Humans; D007150:Immunohistochemistry; D007677:Kidney Function Tests; D016030:Kidney Transplantation / Q000473:pathology / Q000502:physiology*; D011065:Poly(ADP-ribose) Polymerases / Q000032:analysis* / Q000235:genetics; D011237:Predictive Value of Tests; D014019:Tissue Donors
chemical_list: D011065:Poly(ADP-ribose) Polymerases