title: Structural, cytochemical, immunocytochemical and ultrastructural characterization of blood granulocytes of the roadside hawk Buteo magnirostris (Gmelin, 1788) (Avian, Falconiform).
abstract: In the peripheral blood of the roadside hawk, Buteo magnirostris, the following types of granulocytic leucocytes were identified: heterophil, eosinophil and basophil. The heterophils presented acidophilic and spindle shaped granules, the eosinophils possess spherical eosinophilic granules and the basophils showed spherical and basophilic granules. The heterophils and eosinophils presented positive cytochemical reaction to glycogen and basic polyaminoacid, while the eosinophils presented sudanophilic granules, which were also positive for myeloperoxidase. The heterophils, alone, presented positivity for acid phosphatase in some granules and immunoreactivity to TGF-beta1 was observed only in the cytoplasm of the eosinophils. Electron microscopy demonstrated the heterophil granules as predominantly spindle shaped, being strongly electron-dense, while the eosinophils had numerous uniformly electron-dense spherical granules and the basophils presented three different types of granules identified according to their electron-density and the aspect of their matrix.
authors.affiliation: Morphology Department of the Adventist University Center of São Paulo, UNASP, Brazil. antenors@iae-sp.br
authors.forename: A A
authors.initials: AA
authors.lastname: Santos
authors.forename: A Marques Joppert
authors.initials: AM
authors.lastname: Da Silva
authors.forename: M R Regis
authors.initials: MR
authors.lastname: Silva
authors.forename: H R Cômodo
authors.initials: HR
authors.lastname: Segreto
authors.forename: M Imoto
authors.initials: MI
authors.lastname: Egami
mesh_terms: D000818:Animals; D001491:Basophils / Q000166:cytology / Q000276:immunology / Q000502:physiology / Q000648:ultrastructure; D004804:Eosinophils / Q000166:cytology / Q000276:immunology / Q000502:physiology / Q000648:ultrastructure; D006098:Granulocytes / Q000166:cytology / Q000276:immunology / Q000502:physiology* / Q000648:ultrastructure; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D020304:Raptors / Q000276:immunology / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000276:immunology
chemical_list: D016212:Transforming Growth Factor beta