Influence of thyroid status on the differentiation of slow and fast muscle phenotypes.
Muscle phenotype is determined by combined effects of intrinsic genetic and extrinsic factors like innervation, hormonal levels and mechanical factors or muscle activity. We have been studying the effect of altered thyroid hormone levels on the expression of myosin heavy chain (MyHC) isoforms in control and regenerating soleus and extensor digitorum longus muscles of euthyroid, hypothyroid or hyperthyroid female inbred Lewis rats. The fiber type composition has been determined according to the mATPase activity and immunocytochemical staining of MyHC isoforms, the content of MyHC isoforms has been determined by SDS-PAGE, the mRNA levels have been measured by RT-PCR and the ultrastructural transformation has been analyzed by electron-microscopy. Our results indicate that although the innervation plays a decisive role in the determination of muscle phenotype, levels of thyroid hormones contribute to the extent of muscle phenotype transformation.
Vadászová
A
A
Department of Functional Morphology, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Zacharová
G
G
Machácová
K
K
Jirmanová
I
I
Soukup
T
T
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D005260:Female; D006980:Hyperthyroidism / Q000235:genetics; D007037:Hypothyroidism / Q000235:genetics; D018656:Muscle Fibers, Fast-Twitch / Q000502:physiology; D018657:Muscle Fibers, Slow-Twitch / Q000502:physiology; D018482:Muscle, Skeletal / Q000502:physiology* / Q000648:ultrastructure; D018995:Myosin Heavy Chains / Q000235:genetics / Q000502:physiology*; D010641:Phenotype; D020033:Protein Isoforms / Q000235:genetics; D051381:Rats; D011917:Rats, Inbred Lew; D019831:Sodium-Calcium Exchanger / Q000235:genetics; D013963:Thyroid Hormones / Q000235:genetics / Q000502:physiology*
D020033:Protein Isoforms; D019831:Sodium-Calcium Exchanger; D013963:Thyroid Hormones; D018995:Myosin Heavy Chains