[The expression and significance of major histocompatibility complex II transactivator gene in five human malignant hematological cell lines].
OBJECTIVE
To investigate the role of major histocompatibility complex (MHC) II transactivator in constitutive or interferon (IFN)-gamma inducible expression of MHC molecules in human malignant hematological cell lines.


METHODS
The expression of MHC molecules and MHC class II transactivator (CIITA) protein were detected with Western blot, immunohistochemistry and flow cytometry. The expression of CIITA gene was determined with RT-PCR. T cell expressing interleukin (IL)-2 mRNA was induced by Jurkat with mixed lymphocyte reaction.


RESULTS
It was found that the MHCII-positive tumor cells expressed the CIITA quite well, and IFN-gamma could induce the expression of MHCI + II only if the tumor cells were able to express CIITA in an IFN-gamma induced manner (20%, 42% for Jurkat cells respectively), while T cell expressed more IL-2 mRNA (51 times) in the case of Jurkat after induction with IFN-gamma.


CONCLUSIONS
It is suggested that there is a correlation between the inability of the tumor cells in response to IFN-gamma for MHC expression and the deficiency in the inducible expression of CIITA. This might be due to immune sneaking through the tumor cells to some extent.
You
Yong
Y
Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. youuion@medmail.com.cn
Zou
Ping
P
Guo
Rong
R
D015153:Blotting, Western; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D018922:HL-60 Cells; D019337:Hematologic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000494:pharmacology; D007376:Interleukin-2 / Q000235:genetics; D019169:Jurkat Cells; D020014:K562 Cells; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D020298:U937 Cells
D007376:Interleukin-2; C083728:MHC class II transactivator protein; D009687:Nuclear Proteins; D012333:RNA, Messenger; D015534:Trans-Activators; D007371:Interferon-gamma