[The effects of chitosan on different-derived fibroblasts].
OBJECTIVE
To investigate the effects of chitosan on the biological activities of the fibroblasts derived from different tissues.


METHODS
The biological activities of the fibroblasts derived from different tissues were evaluated with a MTT method for fibroblast proliferation, photic and electronic microscope for morphologic and subcellular structure, 3H-proline uptake method for collagen secretion and ELISA box for the secretion of TGF-beta 1, FGF-AB, and IL-8.


RESULTS
This study showed that the chitosan inhabited the proliferation of the fibroblasts and the secretion of the TGF-beta 1, FGF-AB and collagen of the fibroblasts with a dose-depended manner in the normal skin, hypertrophic scar and keloid groups, but it stimulated the IL-8. However, there were no significant differences among the three groups (P > 0.05).


CONCLUSIONS
The chitosan could inhibit the growth, proliferation, biosynthesis and secretion of the fibroblasts, and it may be used to treat different scars.
Zhang
Jing-De
JD
Department of Plastic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Xing
Xin
X
Sun
Mei-qing
MQ
Song
Jian-xing
JX
Ouyang
Tian-xiang
TX
D000293:Adolescent; D000328:Adult; D002455:Cell Division / Q000187:drug effects; D002686:Chitin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D048271:Chitosan; D004305:Dose-Response Relationship, Drug; D005260:Female; D005346:Fibroblast Growth Factors / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D006490:Hemostatics / Q000494:pharmacology; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D010446:Peptide Fragments / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism
D006490:Hemostatics; D016209:Interleukin-8; D010446:Peptide Fragments; D016212:Transforming Growth Factor beta; D002686:Chitin; D005346:Fibroblast Growth Factors; D048271:Chitosan; C052888:fibroblast growth factor (1-10)