Oxidative DNA damage in placentas from normal and pre-eclamptic pregnancies.
Placental oxidative stress was suggested to play a role in the pathogenesis of pre-eclampsia (PE). In this study, levels of 8-hydroxy-2'-deoxyguanosine (8-OH-dG), a well-established marker of oxidative DNA damage, were analysed in placental cellular DNA from normal (group NP) and pre-eclamptic (group PE) pregnancies as well as from PE pregnancies complicated by intrauterine growth restriction (group PE-IUGR). Placental samples obtained immediately after delivery were frozen at -80 degrees C until analysis. Cellular DNA was isolated, hydrolysed and analysed using high-performance liquid chromatography. Native nucleosides were monitored at 254 nm and 8-OH-dG using electrochemical detection. Concentrations of 8-OH-dG were expressed as micro mol/mol 2'-deoxyguanosine. In group NP, mean concentration of 8-OH-dG reached 179.97+/-80.58 (+/-SEM; micro mol/mol dG). 8-OH-dG levels were higher in group PE (273.44+/-110.14 micro mol/mol), but the difference was not significant in comparison with group NP. Highest concentrations of 8-OH-dG were found in group PE-IUGR (428.97+/-141.40 micro mol/mol), with levels significantly higher than in group NP, but not group PE. The results indicate a positive correlation between the severity of PE and the degree of oxidative stress and corroborate previous studies suggesting reactive oxygen species to be involved in the pathophysiology of PE.
Wiktor
Henryk
H
Department of Obstetrics and Gynaecology, Medical University School, Lublin, Poland.
Kankofer
Marta
M
Schmerold
Ivo
I
Dadak
Agnes
A
Lopucki
Maciej
M
Niedermüller
Hans
H
D000080242:8-Hydroxy-2'-Deoxyguanosine; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D005260:Female; D005317:Fetal Growth Retardation / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007231:Infant, Newborn; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010920:Placenta / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D011225:Pre-Eclampsia / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy
D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine