Fast screening method for detection of acyl-HSL-degrading soil isolates.
A reliable method was developed for screening of bacteria isolates capable of degrading acyl-HSLs, the signal molecules in quorum-sensing-mediated processes of many Proteobacteria. The microtiter assay was based on the use of a GFP-marked Escherichia coli strain, which fluoresces upon the presence of acyl-HSLs. Measurement of GFP fluorescence with a Molecular Imager FX scanner (fluorometer) detected isolates capable of degrading acyl-HSLs. The potential of this method was demonstrated by isolation of different bacteria from a potato rhizosphere able to inactivate synthetic and natural acyl HSLs produced by Pectobacterium carotovorum subsp. carotovorum (Pcc) (Erwinia carotovora subsp. carotovora (Ecc)).
Jafra
Sylwia
S
Plant Research International, P.O. Box 16, 6700 AA Wageningen, The Netherlands. jafra@biotech.univ.gda.pl
van der Wolf
Jean Martin
JM
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000378:metabolism*; D016960:Agrobacterium tumefaciens / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D005470:Fluorometry; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D016973:Pectobacterium carotovorum / Q000378:metabolism*; D012988:Soil Microbiology; D011198:Solanum tuberosum
D008164:Luminescent Proteins; C088386:homoserine lactone; D049452:Green Fluorescent Proteins; D015107:4-Butyrolactone