title: Paratuberculosis in red deer (Cervus elaphus): an immunohistochemical study.
abstract: In the present study, we compared the utility of immunohistochemistry with serological and histological results for the characterization of Mycobacterium avium ssp. paratuberculosis (M. paratuberculosis) in tissues of affected red deer. Bacterial isolation was considered the standard reference. Samples were taken from seven clinically affected animals with typical macroscopic lesions. The enzyme linked immunosorbent assay (ELISA) and the gel diffusion tests (GD) were used for serological determinations. Samples from intestine and mesenteric lymph nodes were processed for bacterial isolation and histology. M. paratuberculosis was isolated from all the animals. Histologically, lymph nodes displayed necrosis and mineralization at the cortical and medullar areas. Ziehl-Neelsen stained bacteria were numerous inside macrophages and Langhans-type giant cells. Giant and epithelioid cells and lymphocytes were prominent at the ileal mucous membrane. The immunostaining of M. paratuberculosis was very clear inside epithelioid and giant cells. Image analysis was carried out to determine the immunostained area. There was total agreement among the methods employed. Immunohistochemistry can be very useful when the microorganism cannot be recovered from tissues or faeces.
authors.affiliation: Department of Animal Production, National University of Mar del Plata, EEA INTA Balcarce, PO Box 276, 7620 Balcarce, Argentina. fpaolicchi@balcarce.inta.gov.ar
authors.forename: F A
authors.initials: FA
authors.lastname: Paolicchi
authors.forename: A
authors.initials: A
authors.lastname: Vagnozzi
authors.forename: C G
authors.initials: CG
authors.lastname: Morsella
authors.forename: A E
authors.initials: AE
authors.lastname: Verna
authors.forename: A R
authors.initials: AR
authors.lastname: Massone
authors.forename: E L
authors.initials: EL
authors.lastname: Portiansky
authors.forename: E J
authors.initials: EJ
authors.lastname: Gimeno
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D004269:DNA, Bacterial / Q000032:analysis; D003670:Deer; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000382:microbiology; D005779:Immunodiffusion / Q000662:veterinary; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary*; D008198:Lymph Nodes / Q000382:microbiology; D016927:Mycobacterium avium subsp. paratuberculosis / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D010283:Paratuberculosis / Q000382:microbiology* / Q000473:pathology
chemical_list: D000907:Antibodies, Bacterial; D004269:DNA, Bacterial