title: [Effect of PD98059 on Ras-MAPK signal transduction pathway of chronic myelogenous leukemia].
abstract: OBJECTIVE
  To study the effect and mechanism of PD98059 on Ras-MAPK signal transduction pathway of chronic myelogenous leukemia.
  
  
  METHODS
  K562 cell line was treated by PD98059, cell viability, DNA synthesis, colony formation and MAPK activity of the treated cells were analyzed.
  
  
  RESULTS
  The cell viability, DNA synthesis, colony formation and MAPK activity were significantly inhibited by PD98059 (P<0.05), and the inhibitory effect was dose dependent.
  
  
  CONCLUSIONS
  The inhibitory effect of PD98059 was achieved by blocking Ras-MAPK signal transduction pathway which can become a new target in the treatment of CML.
authors.affiliation: Hematology Department of Xijing Hospital, Fourth Military Medical University,Xi'an 710032,China.
authors.forename: Zhen-chuan
authors.initials: ZC
authors.lastname: Shang
authors.forename: Bing-zhong
authors.initials: BZ
authors.lastname: Sun
authors.forename: Zhi-nan
authors.initials: ZN
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Qi
authors.initials: Q
authors.lastname: Feng
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Sha
authors.initials: S
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D020014:K562 Cells; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D015398:Signal Transduction; D018631:ras Proteins / Q000378:metabolism*
chemical_list: D004273:DNA, Neoplasm; D005419:Flavonoids; D020928:Mitogen-Activated Protein Kinases; D018631:ras Proteins; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one