title: Improved antiviral activity in vitro of ribavirin against measles virus after complexation with cyclodextrins.
abstract: Despite vaccination, measles remains a burden in both developed and developing countries and complications may necessitate an efficient therapy. Measles virus (MEV) is susceptible to ribavirin (RBV), but the use of this drug is limited by its toxicity. Cyclodextrins (CDs) can form complexes with numerous molecules, improving their bioavailability and their biological properties. We have evaluated in vitro the antiviral effects of complexes of RBV with alpha-, beta- or gamma-CD against two clade A laboratory strains of MEV (Edmonston and CAM/RB) grown on Vero cells. Complexation of RBV with alpha-CD or beta-CD lead to a five-fold or a two-fold decrease in the 50% inhibitory concentration, respectively, against both MEV strains. In contrast, gamma-CD complexation showed no modification.
authors.affiliation: Groupe d'Etude des Vecteurs Supramoléculaires du Médicament, Unité Mixte de Recherche Université-CNRS 7565, Structure et Réactivité des Systèmes Moléculaires Complexes, Université Henri Poincaré, Nancy 1, France. nicolas.grancher@wanadoo.fr
authors.forename: Nicolas
authors.initials: N
authors.lastname: Grancher
authors.forename: Véronique
authors.initials: V
authors.lastname: Venard
authors.forename: Francine
authors.initials: F
authors.lastname: Kedzierewicz
authors.forename: Wim
authors.initials: W
authors.lastname: Ammerlaan
authors.forename: Chantal
authors.initials: C
authors.lastname: Finance
authors.forename: Claude P
authors.initials: CP
authors.lastname: Muller
authors.forename: Alain
authors.initials: A
authors.lastname: Le Faou
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D003505:Cyclodextrins / Q000378:metabolism / Q000494:pharmacology*; D004339:Drug Compounding; D004355:Drug Stability; D066298:In Vitro Techniques; D008459:Measles virus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D012254:Ribavirin / Q000378:metabolism / Q000494:pharmacology*; D014709:Vero Cells
chemical_list: D003505:Cyclodextrins; D012254:Ribavirin