Protein-coated poly(L-lactic acid) fibers provide a substrate for differentiation of human skeletal muscle cells.
Tissue engineering represents a potential method for repairing damaged skeletal muscle tissue. Extracellular matrix (ECM) proteins were evaluated for their ability to aid in cell attachment, whereas a poly(L-lactic acid) (PLLA) fiber scaffold was tested as a substrate for the differentiation of human skeletal muscle cells. In comparison to uncoated or gelatin-coated PLLA films, cell attachment increased significantly (p < 0.001) on PLLA films coated with ECM gel, fibronectin, or laminin. Myoblasts differentiated into multinucleated myofibers on ECM gel-coated PLLA fibers, and expressed muscle markers such as myosin and alpha-actinin. Oligonucleotide microarray analysis showed similar gene expression profiles for human skeletal muscle cells on ECM gel-coated PLLA fibers as to that observed for myofibers on tissue culture plates. Therefore, PLLA fibers coated with ECM proteins provide a scaffold for the development of skeletal muscle tissue for tissue engineering and cell transplantation applications.
Cronin
Elizabeth M
EM
Joint Biomedical Engineering Program, University of Texas Southwestern Medical Center, Dallas, and University of Texas at Arlington, Arlington, USA.
Thurmond
Frederick A
FA
Bassel-Duby
Rhonda
R
Williams
R Sanders
RS
Wright
Woodring E
WE
Nelson
Kevin D
KD
Garner
Harold R
HR
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry / Q000378:metabolism*; D008422:Materials Testing; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology* / Q000502:physiology; D032448:Myoblasts, Skeletal / Q000166:cytology / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods
D016326:Extracellular Matrix Proteins; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)