Effect of 2'-deoxyisoguanosine on the growth of human tumor and normal cell lines.
OBJECTIVE
To test the effect of 2'-deoxyisoguanosine as a specific substrate of HIV reverse transcriptase on the growth of cultured normal and cancer cells.


METHODS
The effect of 2'-deoxyisoguanosine on the growth of cells of two normal (telomerase-negative) and five cancer cell lines have been tested. Cell viability was assessed by MTT assay.


RESULTS
We have found that this nucleoside analogue has low potency and specificity in inhibiting tumor cell growth. IC50 ranged from 0.5 mM to 2 mM for tumor cells, and from 1 mM to more than 4 mM for normal cells.


CONCLUSIONS
2'-deoxyisoguanosine has low potency and specificity in inhibiting tumor cell growth, similar to other telomerase inhibitors.
Nowak
Radoslawa
R
Department of Molecular Biology, Maria Sklodowska-Curie Memorial Cancer Center, Institute of Oncology, Warsaw, Poland. radka@coi.waw.pl
Sawardo-Rochowska
Maria
M
Kazimierczuk
Zygmunt
Z
Nowak
Roman J
RJ
D000241:Adenosine; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D006151:Guanosine / Q000494:pharmacology*; D006801:Humans; D020128:Inhibitory Concentration 50; D013379:Substrate Specificity; D019098:Telomerase / Q000378:metabolism
D006151:Guanosine; C008184:isoguanosine; D019098:Telomerase; D000241:Adenosine