Evaluation of antiviral activity against human herpesvirus 8 (HHV-8) and Epstein-Barr virus (EBV) by a quantitative real-time PCR assay.
A real-time quantitative PCR was developed to assess antiviral activity of molecules against human herpesvirus 8 (HHV-8) and the Epstein-Barr virus (EBV). The antiviral activity of the reference molecules acyclovir, ganciclovir, cidofovir, adefovir and brivudin, as assessed by this methodology, proved very similar to the activity as determined by a DNA-DNA hybridisation method.
Friedrichs
Claudia
C
Institute of Virology and Antiviral Therapy, Friedrich-Schiller University of Jena, Jena, Germany.
Neyts
Johan
J
Gaspar
Gabor
G
De Clercq
Erik
E
Wutzler
Peter
P
D000212:Acyclovir / Q000494:pharmacology; D000998:Antiviral Agents / Q000097:blood / Q000494:pharmacology*; D002460:Cell Line; D000077404:Cidofovir; D003596:Cytosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D015774:Ganciclovir / Q000494:pharmacology; D004854:Herpesvirus 4, Human / Q000187:drug effects* / Q000235:genetics / Q000276:immunology; D019288:Herpesvirus 8, Human / Q000187:drug effects* / Q000235:genetics / Q000276:immunology; D006801:Humans; D008826:Microbial Sensitivity Tests; D063065:Organophosphonates; D009943:Organophosphorus Compounds / Q000494:pharmacology; D016133:Polymerase Chain Reaction
D000998:Antiviral Agents; D063065:Organophosphonates; D009943:Organophosphorus Compounds; D003596:Cytosine; D000077404:Cidofovir; D015774:Ganciclovir; D000212:Acyclovir