Identification of virus-encoded microRNAs.
RNA silencing processes are guided by small RNAs that are derived from double-stranded RNA. To probe for function of RNA silencing during infection of human cells by a DNA virus, we recorded the small RNA profile of cells infected by Epstein-Barr virus (EBV). We show that EBV expresses several microRNA (miRNA) genes. Given that miRNAs function in RNA silencing pathways either by targeting messenger RNAs for degradation or by repressing translation, we identified viral regulators of host and/or viral gene expression.
Pfeffer
Sébastien
S
Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, NY 10021, USA.
Zavolan
Mihaela
M
Grässer
Friedrich A
FA
Chien
Minchen
M
Russo
James J
JJ
Ju
Jingyue
J
John
Bino
B
Enright
Anton J
AJ
Marks
Debora
D
Sander
Chris
C
Tuschl
Thomas
T
D000818:Animals; D002144:Callithrix; D002460:Cell Line; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D019295:Computational Biology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000502:physiology; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D020506:Untranslated Regions; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D017735:Virus Latency
C083278:BHRF1 protein, Human herpesvirus 4; D004268:DNA-Binding Proteins; D035683:MicroRNAs; D011506:Proteins; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D012367:RNA, Viral; D020506:Untranslated Regions; D014764:Viral Proteins; C083202:BALF5 protein, Epstein-barr virus; D004259:DNA-Directed DNA Polymerase