[Interaction of viral and cellular genes in cervical tumors].
The results obtained in the Laboratory of Molecular Biology of Viruses, CRC carried out in the framework of the Human Genome program and devoted to the study of the activity of cell and viral genes in cervical cancer are summarized. DNA of human papillomaviruses persists in tumors both in episomal and integrative forms. Integration may occur in different regions of chromosomes. Viral transforming genes E6 and E7 are always present in tumor cells, while antibodies to these proteins are detected only in approximately 30% of patients. Loss of heterozygosity is detected on long and short arms of chromosome 6; some such cases are manifest already at the early stages of tumor progression, while others are typical of the late stages. Several genes that are potentially involved in tumorigenesis and are subject to hypermethylation in CpG islands were identified. Methylation of several genes is observed in approximately 30% of tumors. Tumor progression is associated with increased expression of p16ink4a, an inhibitor of cyclin-dependent kinases.
Kiselev
F L
FL
Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Moscow, 115478 Russia. f_kiss@crc.umos.ru
Mazurenko
N N
NN
Volgareva
G M
GM
Kislelev
N P
NP
D019175:DNA Methylation; D005260:Female; D005814:Genes, Viral; D006801:Humans; D019656:Loss of Heterozygosity; D027383:Papillomaviridae / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000821:virology*