DNA microarrays as a tool in toxicogenomics.
Toxicogenomics is an emerging technology that defines the use of novel genomic techniques to investigate the adverse effects of xenobiotic on gene expression. Toxicogenomics is based on the fact that most of relevant toxicological effects of a compound affect directly or indirectly the gene expression. The most common methods to profile gene expression at the transcript level are Northern Blotting and the real-time PCR. While commonly used and well accepted, these techniques are now superseded by new technologies allowing the analysis of the expression for multiple genes simultaneously. DNA microarrays are now developed for simultaneous gene analysis but inherent to such multiple assays, their quantitative aspect and their relevance for toxicogenomics have been questioned. We will review here recent studies on their use for toxicogenomics and examine the possible future of such technology in complementation with the other toxicology methods.
de Longueville
F
F
Eppendorf Array Technologies, 20 rue du séminaire, 5000 Namur, Belgium. delongueville.f@eppendorf.be
Bertholet
V
V
Remacle
J
J
D000818:Animals; D020869:Gene Expression Profiling; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D004364:Pharmaceutical Preparations / Q000378:metabolism; D011237:Predictive Value of Tests; D043922:Toxicogenetics; D014158:Transcription, Genetic; D015262:Xenobiotics / Q000633:toxicity
D004364:Pharmaceutical Preparations; D015262:Xenobiotics