title: Depletion of methionine aminopeptidase 2 does not alter cell response to fumagillin or bengamides.
abstract: Inhibition of endothelial cell growth by fumagillin has been assumed to be mediated by inhibition of the molecular target methionine aminopeptidase 2 (MetAp2). New data show that depletion of MetAp2 by siRNA does not inhibit endothelial cell growth. Moreover, MetAp2-depleted endothelial cells remain responsive to inhibition by either fumagillin or a newly identified MetAp2 enzyme inhibitor. These data suggest that MetAp2 function is not required for endothelial cell proliferation.
authors.affiliation: Novartis Pharmaceuticals, East Hanover, New Jersey 07936, USA.
authors.forename: Sunkyu
authors.initials: S
authors.lastname: Kim
authors.forename: Kenneth
authors.initials: K
authors.lastname: LaMontagne
authors.forename: Michael
authors.initials: M
authors.lastname: Sabio
authors.forename: Sushil
authors.initials: S
authors.lastname: Sharma
authors.forename: Richard W
authors.initials: RW
authors.lastname: Versace
authors.forename: Naeem
authors.initials: N
authors.lastname: Yusuff
authors.forename: Penny E
authors.initials: PE
authors.lastname: Phillips
mesh_terms: D000626:Aminopeptidases / Q000037:antagonists & inhibitors / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D001381:Azepines / Q000494:pharmacology*; D002289:Carcinoma, Non-Small-Cell Lung / Q000201:enzymology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D003510:Cyclohexanes; D004730:Endothelium, Vascular / Q000187:drug effects / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D012717:Sesquiterpenes; D013379:Substrate Specificity; D014162:Transfection
chemical_list: C438868:3,4,5-trihydroxy-2--methoxy-8,8-dimethyl-N-(hexahydro-2-oxo-6-(cyclohexylcarbonyl)oxy-2H-azepin-3-yl)non-6-enamide; D001381:Azepines; D003510:Cyclohexanes; D004791:Enzyme Inhibitors; D005231:Fatty Acids, Unsaturated; D034741:RNA, Small Interfering; D012717:Sesquiterpenes; C026211:fumagillin; D000626:Aminopeptidases; C106541:methionine aminopeptidase 2; D008666:Metalloendopeptidases