[Investigation of primary cell culture for dental pulp: comparing of coronal pulp and root pulp].
OBJECTIVE
To compare and establish an ideal method for the culture of human primary dental coronal and root pulp cells.


METHODS
The whole dental pulp tissues were obtained from healthy and young human teeth extracted for orthodontic purposes. The cells in coronal and root pulp were separately cultured by the tissue explant and tissue explant-collagenase digestion(0.1% type I collagenase) methods. By evaluating the attachment efficiency,viability and the successfulness of the cultured cells, the optimal primary cell culture process of human dental coronal and root pulp was determined.


RESULTS
The tissue explant culture had more successfulness,more viability and less attachment efficiency than that of tissue explant-collagenase digestion method in the culture of human coronal and root dental pulp cells.


CONCLUSIONS
Tissue explant method was superior to tissue explant-collagenase digestion method for the culturing of human dental coronal and root pulp cells. The ideal method for dental coronal and root pulp cells was established in this study.
Liu
Shao-hua
SH
Oral and Maxillofacial Department, Qilu Hospital of Shangdong University,Jinan 250012, Shandong province, China. lshabccba@sdu.edu.cn
Wei
Feng-cai
FC
Sun
Shan-zhen
SZ
Zhang
Chun-yan
CY
Liu
Yun-sheng
YS
D000293:Adolescent; D000328:Adult; D018929:Cell Culture Techniques / Q000379:methods*; D003782:Dental Pulp / Q000166:cytology*; D006801:Humans