Saturated fatty acids suppress adrenocorticotropic hormone (ACTH) release from rat anterior pituitary cells in vitro.
We studied whether fatty acids modify adrenocorticotropic hormone (ACTH) release induced by stimulation with corticotropin-releasing hormone (CRH) from rat anterior pituitary cells. Stimulation with CRH (0.01-100 nmol/l) significantly and concentration-dependently increased ACTH release, which was synergistically enhanced by the simultaneous stimulation with 1 nmol/l arginine-vasopressin. Addition of saturated fatty acids (butyrate, caprylate, laurate, palmitate and stearate) in a medium at 1 mmol/l, despite effects on the basal release, significantly reduced the ACTH release induced by CRH (1 nmol/l) stimulation. Caprylate suppressed ACTH release in a concentration-dependent manner. However, unsaturated C18 and C20 fatty acids (oleate, linolate, linolenate and arachidonate) at 1 mmol/l significantly increased the basal release, but none of them suppressed CRH (1 nmol/l)-induced ACTH release. In the presence of caprylate (1 mmol/l), CRH (1 nmol/l)-stimulated increase in cellular calcium ion concentration was diminished. From these results we conclude that saturated fatty acids have a suppressing effect on CRH-induced ACTH increase in primary cultured rat anterior pituitary cells.
Katoh
Kazuo
K
Department of Animal Physiology, Postgraduate School of Agriculture, Tohoku University, Tsutsumidori, Aoba-ku, Sendai 981-8555, Japan. kato@mail.bios.tohoku.ac.jp
Asari
Mami
M
Ishiwata
Hiroko
H
Sasaki
Yasuyuki
Y
Obara
Yoshiaki
Y
D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D001127:Arginine Vasopressin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002210:Caprylates / Q000494:pharmacology; D002478:Cells, Cultured; D003346:Corticotropin-Releasing Hormone / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D005227:Fatty Acids / Q000494:pharmacology* / Q000502:physiology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology / Q000502:physiology; D018613:Microscopy, Confocal; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism*; D051381:Rats
D002210:Caprylates; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D001127:Arginine Vasopressin; D016718:Arachidonic Acid; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone; C031492:octanoic acid; D002118:Calcium