[Preparation and identification of monoclonal antibodies against CD100 molecule].
OBJECTIVE
To prepare and identify the monoclonal antibodies (mAbs) against CD100.


METHODS
BALB/c mice were immunized with eukaryotic expressed CD100 protein. The splenocytes from immunized mice were fused with Sp2/0 cells and the positive hybridoma clones were screened by indirect ELISA and mAbs reacting to CD100 antigen were identified by indirect immunofluorescent staining and flow cytometry with human acute T lymphoblastic leukemia cell line MOLT-4, activated human PBMC, gibbon T lymphocyte cell line MLA-144 and monkey EBV-transformed B lymphoblast cell line(BLCL).


RESULTS
Seven hybridomas secreting mAbs against CD100 molecule were obtained. All the mAbs could react to natural CD100 expressed on human MOLT-4 and activated PBMCs as well as monkey BLCL cells, and six out of them could react to gibbon MLA-144 cells.


CONCLUSIONS
A set of mAbs recognizing CD100 molecule expressed on human, monkey and gibbon cells are obtained, which may provide an useful tool for studying the structure-function relationship of CD100 molecule of human, as well as gibbon and monkey.
Xu
Xiao-guang
XG
Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.
Zhu
Yong
Y
Liu
Xue-song
XS
Tian
Ying
Y
Cao
Yun-xin
YX
Hu
Yi
Y
Jin
Bo-quan
BQ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology*; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D000882:Haplorhini; D006801:Humans; D006825:Hybridomas / Q000276:immunology / Q000378:metabolism; D006922:Hylobates; D015458:Leukemia, T-Cell / Q000276:immunology / Q000473:pathology*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D039961:Semaphorins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C093224:CD100 antigen; D039961:Semaphorins