title: Prophylactic effect of IL-10 gene transfer on induced autoimmune dacryoadenitis.
abstract: OBJECTIVE
  To evaluate the effect of viral IL-10 on the lacrimal gland immunopathologic response in the ocular surface disease, induced autoimmune dacryoadenitis.
  
  
  METHODS
  Disease was induced in rabbits by injecting inferior lacrimal glands with peripheral blood lymphocytes activated by 5 days of coculture with autologous acinar cells in a mixed-cell reaction. In the treated group, an adenoviral vector carrying the vIL-10 gene was concurrently injected with activated lymphocytes. Tears were collected periodically for quantitation of IL-10 by ELISA. Two weeks after disease induction, tear production, tear film breakup time, and rose bengal staining score were determined. Sectioned glands were immunostained for expression of CD4, CD8, rabbit thymic lymphocyte antigen (RTLA), CD18 and major histocompatibility complex class II.
  
  
  RESULTS
  The titer of vIL-10 in tears was at its maximum on day 3, started to decline by day 7, and was undetectable by day 14. In the diseased group, the tear production rate and tear film breakup time were significantly decreased, and rose bengal staining was significantly increased. Diseased glands had immune cell infiltrates containing CD4+, RTLA+, and CD18+ cells, and major histocompatibility complex class II expression was increased. These changes were significantly ameliorated by expression of vIL-10.
  
  
  CONCLUSIONS
  In vivo transduction of the lacrimal gland with AdvIL-10 resulted in the transient appearance of vIL-10 in tears. The presence of vIL-10 partially suppressed the appearance of Sjögren-syndrome-like features of reduced tear production, accelerated tear breakup, ocular surface disease, and immunopathologic response. Anti-inflammatory cytokine gene expression may offer a therapeutic modality for the treatment of autoimmune dacryoadenitis, once suitable vectors become available.
authors.affiliation: Doheny Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA.
authors.forename: Zejin
authors.initials: Z
authors.lastname: Zhu
authors.forename: Douglas
authors.initials: D
authors.lastname: Stevenson
authors.forename: Joel E
authors.initials: JE
authors.lastname: Schechter
authors.forename: Austin K
authors.initials: AK
authors.lastname: Mircheff
authors.forename: Thomas
authors.initials: T
authors.lastname: Ritter
authors.forename: Laurie
authors.initials: L
authors.lastname: Labree
authors.forename: Melvin D
authors.initials: MD
authors.lastname: Trousdale
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001327:Autoimmune Diseases / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D018821:CD18 Antigens / Q000378:metabolism; D015704:CD4 Antigens / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D003607:Dacryocystitis / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007124:Immunoenzyme Techniques; D016753:Interleukin-10 / Q000235:genetics* / Q000378:metabolism; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D011817:Rabbits; D012859:Sjogren's Syndrome / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D013666:Tears / Q000378:metabolism
chemical_list: D018821:CD18 Antigens; D015704:CD4 Antigens; D016827:CD8 Antigens; D000949:Histocompatibility Antigens Class II; D016169:Lymphocyte Function-Associated Antigen-1; D016753:Interleukin-10