[Immortalized cell lines from murine embryos are characterized by progressive destabilization of their karyotype structure].
The karyotype structure was studied for three cell lines obtained from cells of transgenic murine embryos at early stages of their establishment. The first line was obtained from a transgenic embryonic explantant containing oncogen v-sis under promotor MMTV, two other lines originated from cells of transgenic embryos containing oncogen k51. The karyotypic analysis of G-banded metaphase chromosomes revealed deviations from the normal mouse karyotype as early as by the third passage of cultivation of independent embryonic cell lines that contained a foreign oncogene in their genome. The repeated analysis that involved 15-22 passages revealed similar abnormalities: variability and progression in chromosome number with the appearance of hyperpolyploid combinations, and a large number of rearranged chromosomes, both marker and unique ones. It is concluded that introduction of a foreign oncogene into murine cell genome leads to its enhanced and progressive non-specific destabilization. Oncogen v-sis produces a more valuable karyotype destabilization than oncogen k51.
Grinchuk
T M
TM
Institute of Cytology RAS, St. Petersburg. gmdmk@mail.cytspb.rssi.ru
Pugovkina
N A
NA
Tarunina
M V
MV
Sorokina
E A
EA
Ignatova
T N
TN
D000818:Animals; D002460:Cell Line; D002875:Chromosomes / Q000235:genetics*; D004622:Embryo, Mammalian; D020571:Genes, sis / Q000235:genetics*; D007621:Karyotyping; D008677:Metaphase; D051379:Mice; D008822:Mice, Transgenic; D011003:Ploidies