title: Culture-independent species typing of neotropical Leishmania for clinical validation of a PCR-based assay targeting heat shock protein 70 genes.
abstract: PCR-restriction fragment length polymorphism analysis of heat shock protein 70 genes discriminates most neotropical Leishmania species, as well as Trypanosoma cruzi. The assay, combined with capillary electrophoresis in a microchip device, may be applied directly on clinical samples with a high sensitivity, hence supporting clinical and epidemiological monitoring of leishmaniasis.
authors.affiliation: Prince Leopold Institute of Tropical Medicine, Molecular Parasitology, Antwerp, Belgium.
authors.forename: Lineth
authors.initials: L
authors.lastname: Garcia
authors.forename: Ann
authors.initials: A
authors.lastname: Kindt
authors.forename: Hernan
authors.initials: H
authors.lastname: Bermudez
authors.forename: Alejandro
authors.initials: A
authors.lastname: Llanos-Cuentas
authors.forename: Simonne
authors.initials: S
authors.lastname: De Doncker
authors.forename: Jorge
authors.initials: J
authors.lastname: Arevalo
authors.forename: Kelly
authors.initials: K
authors.lastname: Wilber Quispe Tintaya
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Dujardin
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D017125:Genes, Protozoan; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D007891:Leishmania / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D007896:Leishmaniasis / Q000469:parasitology; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012150:Polymorphism, Restriction Fragment Length; D015800:Protozoan Proteins / Q000235:genetics*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D016054:DNA, Protozoan; D018840:HSP70 Heat-Shock Proteins; D015800:Protozoan Proteins