[Saccharide analysis of si-wu-tang by automated multiple development and off-line coupling thin layer chromatography electrospray ionization mass spectrometry techniques].
OBJECTIVE
To make qualitative analysis on saccharide spots in thin layer chromatography (TLC) chromatogram of SI-WU-TANG extract C, which possesses blood-enrichment activity.


METHODS
TLC chromatogram of SI-WU-TANG extract C was obtained by using Automated Multiple Development (AMD) method. 4 major spots in the chromatogram were analyzed by off-line coupling TLC electrospray ionization mass spectrometry (ESI-MS) technique. Moreover, composition of monosaccharides in the fraction was analyzed by AMD technique.


RESULTS
Main constituents of substances from the 4 spots were monosaccharide, disaccharide, trisaccharide and tetrasaccharide respectively. Monosaccharide was mainly composed of fructose and glucose.


CONCLUSIONS
Off-line coupling TLC ESI-MS can simply and rapidly provide qualitative examination of saccharide spots in TLC chromatogram of Traditional Chinese Medicine. AMD method can make good separation of 8 frequently-observed monosaccharides in a regular 10 cm silica gel plate, the process of which was automated, AMD and off-line coupling TLC ESI-MS techniques show good value in saccharides analysis.
Liang
Qian-de
QD
Institue of Radiation Medicine, Acadamy of Military Medical Sciences, Beijing 100850, China.
Li
Wei-hua
WH
Wang
Hong-xia
HX
Wang
Sheng-qi
SQ
D029971:Angelica sinensis / Q000737:chemistry; D002855:Chromatography, Thin Layer; D004187:Disaccharides / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005632:Fructose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D030004:Ligusticum / Q000737:chemistry; D009005:Monosaccharides / Q000032:analysis* / Q000737:chemistry; D029596:Paeonia / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D032267:Rehmannia / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D014312:Trisaccharides / Q000032:analysis
D004187:Disaccharides; D004365:Drugs, Chinese Herbal; D009005:Monosaccharides; D014312:Trisaccharides; C403258:si-wu-tang; D005632:Fructose; D005947:Glucose