Prediction of survival in diffuse large-B-cell lymphoma based on the expression of six genes.
BACKGROUND
Several gene-expression signatures can be used to predict the prognosis in diffuse large-B-cell lymphoma, but the lack of practical tests for a genome-scale analysis has restricted the use of this method.


METHODS
We studied 36 genes whose expression had been reported to predict survival in diffuse large-B-cell lymphoma. We measured the expression of each of these genes in independent samples of lymphoma from 66 patients by quantitative real-time polymerase-chain-reaction analyses and related the results to overall survival.


RESULTS
In a univariate analysis, genes were ranked on the basis of their ability to predict survival. The genes that were the strongest predictors were LMO2, BCL6, FN1, CCND2, SCYA3, and BCL2. We developed a multivariate model that was based on the expression of these six genes, and we validated the model in two independent microarray data sets. The model was independent of the International Prognostic Index and added to its predictive power.


CONCLUSIONS
Measurement of the expression of six genes is sufficient to predict overall survival in diffuse large-B-cell lymphoma.
Lossos
Izidore S
IS
Division of Oncology, Department of Medicine, Stanford University Medical Center, Stanford, Calif, USA.
Czerwinski
Debra K
DK
Alizadeh
Ash A
AA
Wechser
Mark A
MA
Tibshirani
Rob
R
Botstein
David
D
Levy
Ronald
R
D000293:Adolescent; D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D016393:Lymphoma, B-Cell / Q000188:drug therapy / Q000235:genetics* / Q000401:mortality; D016403:Lymphoma, Large B-Cell, Diffuse / Q000188:drug therapy / Q000235:genetics* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D008957:Models, Genetic; D015233:Models, Statistical; D015999:Multivariate Analysis; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016019:Survival Analysis