Rapid diagnosis of Staphylococcus aureus bacteremia using S. aureus PNA FISH.
In the study presented here, the performance of the S. aureus PNA FISH assay was evaluated using 285 blood cultures (from 104 patients) that had gram-positive cocci resembling staphylococci on Gram stain. The new molecular test is based on a fluorescence in situ hybridization assay using peptide nucleic acid probes targeting Staphylococcus aureus 16S rRNA and is designed for the rapid identification of Staphylococcus aureus directly from positive blood cultures. The sensitivity, specificity, and positive and negative predictive values of the S. aureus PNA FISH for the rapid identification of Staphylococcus aureus directly from positive blood culture bottles were 100, 99.4, 99.2 and 100%, respectively.
González
V
V
Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Carretera. del Canyet s/n, Badalona, 08916 Barcelona, Spain.
Padilla
E
E
Giménez
M
M
Vilaplana
C
C
Pérez
A
A
Fernández
G
G
Quesada
M D
MD
Pallarés
M A
MA
Ausina
V
V
D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology; D001769:Blood / Q000382:microbiology; D003470:Culture Media; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D020135:Peptide Nucleic Acids / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification*; D013997:Time Factors
D003470:Culture Media; D020135:Peptide Nucleic Acids; D012336:RNA, Ribosomal, 16S