Characterization of chromate-sensitive and -tolerant mutants of Schizosaccharomyces pombe.
Stable chromium(VI)-sensitive and -tolerant mutants were obtained by induced mutagenesis of Schizosaccharomyces pombe lysine and leucine auxotrophic heterothallic strains 6chr+ and 9chr+. Eleven of them were selected for further studies. Fast transport of 51CrO4(2-) was detected in a representative sensitive mutant, chr-51S, while the tolerant mutant chr1-66T and the parental strain 6chr+ exhibited significantly lower 51CrO4(2-) uptake. The segregation of tetrads of three selected CrVI-tolerant mutants, chr1-66T, chr1-14T and chr2-04T, strongly indicated that tolerance was determined by single mutations. Random spore analysis proved that the mutations of chr1-66T and chr1-14T were allelic and the mutation of mutant chr2-04T was not allelic with the mutation of chr1-66T. Recombinants carrying the ura4D18 selective marker were created for transformation experiments. Two of them (chr1-661T and chr2-046T) can be used to clone and identify the genes responsible for their CrVI tolerance phenotype.
Czakó-Vér
K
K
Department of General and Environmental Microbiology, Faculty of Science, University of Pécs, 7601 Pécs, Hungary.
Koósz
Z
Z
Antal
J
J
Rácz
T
T
Sipiczki
M
M
Pesti
M
M
D000483:Alleles; D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology; D001692:Biological Transport; D002857:Chromium / Q000378:metabolism* / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003433:Crosses, Genetic; D025141:Drug Resistance, Fungal / Q000235:genetics*; D005799:Genes, Dominant; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D007700:Kinetics; D008670:Metals / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D009154:Mutation; D011995:Recombination, Genetic; D012568:Schizosaccharomyces / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D014170:Transformation, Genetic
D000935:Antifungal Agents; D008670:Metals; D002857:Chromium; C074702:chromium hexavalent ion