title: Evaluation of chlorophyll fluorescence and membrane injury in the leaves of barley cultivars under osmotic stress.
abstract: Two physiological tests for screening drought tolerance of barley (Hordeum vulgare, L.) plants are compared in this work. Water deficit is induced by treating the plants' roots with polyethylene glycol (PEG 8000). The relative water content (RWC) of the plants is used as a measure of the water status. Conductometrically determined electrolyte leakage from the leaf tissue demonstrates the membrane injury caused by dehydration. It is shown that the injury index increases with the decrease of the RWC of the leaves. The F(v)/F(m) ratio is employed to assess changes in the primary photochemical reactions of the photosynthetic apparatus after dehydration. The results suggest that PSII is weakly affected by the imposed osmotic stress. The fluorescence behaviour of the examined cultivars is related to their RWC.
authors.affiliation: Institute of Plant Physiology, Bulgarian Academy of Sciences, Academy G. Bonchev Street, Bl. 21, 1113 Sofia, Bulgaria.
authors.forename: Konstantina
authors.initials: K
authors.lastname: Kocheva
authors.forename: Petar
authors.initials: P
authors.lastname: Lambrev
authors.forename: Georgy
authors.initials: G
authors.lastname: Georgiev
authors.forename: Vasilii
authors.initials: V
authors.lastname: Goltsev
authors.forename: Miroslav
authors.initials: M
authors.lastname: Karabaliev
mesh_terms: D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology*; D002734:Chlorophyll / Q000378:metabolism* / Q000528:radiation effects*; D004190:Disasters; D005453:Fluorescence; D001467:Hordeum / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D007113:Immunity, Innate / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D008027:Light; D009997:Osmotic Pressure / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D010935:Plant Diseases / Q000139:chemically induced; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D011092:Polyethylene Glycols / Q000494:pharmacology; D014867:Water / Q000378:metabolism*; D014882:Water-Electrolyte Balance / Q000187:drug effects / Q000502:physiology
chemical_list: D014867:Water; D002734:Chlorophyll; D011092:Polyethylene Glycols