title: A novel hydrophilic support, CoFoam, for enzyme immobilization.
abstract: Lipases from different sources (porcine pancreas, Mucor miehei and Candida antarctica B) were covalently immobilized on a hydrophilic polyurethane composite (CoFoam). Their hydrolytic activities assayed with tributyrin were 0.55, 2.1 and 447 U g(-1), respectively. The activity of the C. antarctica B composite in the synthesis of methyl oleate in hexane was 8.8 U g(-1) compared to 60.6 U g(-1) for commercial Novozyme 435. The advantage of the CoFoam composite lies in the low pressure drop in a packed-bed reactor at fairly large flow rates. For example, at flow rates of 10-12 l min(-1), the pressure drop over 15 cm is typically 3 kPa.
authors.affiliation: Department of Chemical Engineering, University of New Hampshire, Durham, NH 03824, USA.
authors.forename: P T
authors.initials: PT
authors.lastname: Vasudevan
authors.forename: N
authors.initials: N
authors.lastname: López-Cortés
authors.forename: H
authors.initials: H
authors.lastname: Caswell
authors.forename: D
authors.initials: D
authors.lastname: Reyes-Duarte
authors.forename: F J
authors.initials: FJ
authors.lastname: Plou
authors.forename: A
authors.initials: A
authors.lastname: Ballesteros
authors.forename: K
authors.initials: K
authors.lastname: Como
authors.forename: T
authors.initials: T
authors.lastname: Thomson
mesh_terms: D000818:Animals; D019149:Bioreactors; D002175:Candida / Q000737:chemistry; D002384:Catalysis; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D007700:Kinetics; D008049:Lipase / Q000737:chemistry*; D009089:Mucor / Q000737:chemistry; D010179:Pancreas / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry*; D011312:Pressure; D013379:Substrate Specificity; D013552:Swine
chemical_list: D004800:Enzymes, Immobilized; D011140:Polyurethanes; D008049:Lipase