Egyptian genotyping of Giardia lamblia.
Out of 105 patients infected with Giardia, 38 patients have Genotype I (36.19%), 13 have Genotype II (12.38%), 10 have Genotype III (9.52%), 16 have mixed Genotype infection (15.24%) and 28 with undetermined Giardia infection by PCR (26.67%). None of the control group gave positive results for Giardia in stool by PCR. So, the sensitivity of the test for detection and identification of Giardia Genotypes from the original stool samples was 73.33% and specificity was 100%. Out of 61 cases in the symptomatic group, the prevalence of Giardia Genotype I was 32.79%, Genotype II was 16.39%, Genotype III was 9.84%, mixed Genotype infection was 16.39% and undetermined Genotype was 24.59% as compared to 40.91%, 6.82%, 9.09%, 13.64% & 29.55% in the asymptomatic group respectively. There is statistically insignificant difference between both groups as regarding the prevalence of the different Giardia Genotypes. (P < or = 0.05). The use of PCR as a routine work for diagnosis of giardiasis is not accepted at least in the developing and under-developing countries due to its high cost, the high quality of technical staff and advanced laboratory equipments required for PCR performance. Its application is usually limited to research activities, the detection of water sources contamination and for the detection of a potential source of Giardia infection in epidemics.
El-Shazly
Atef M
AM
Department of Parasitology, Faculties of Medicine, Masoura University, Egypt.
Mowafy
Nawras
N
Soliman
Mohamed
M
El-Bendary
Mahmoud
M
Morsy
Ayman T A
AT
Ramadan
Nashwa I I
NI
Arafa
Wafaa A S
WA
D000293:Adolescent; D000328:Adult; D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis / Q000737:chemistry; D004534:Egypt; D005243:Feces / Q000469:parasitology; D005260:Female; D005838:Genotype; D016829:Giardia lamblia / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005873:Giardiasis / Q000175:diagnosis* / Q000469:parasitology; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity; D014305:Triose-Phosphate Isomerase / Q000235:genetics
D016054:DNA, Protozoan; D014305:Triose-Phosphate Isomerase