Recombinant bacteriophage-based multiepitope vaccine against Taenia solium pig cysticercosis.
The aim of this study was to test the capacity of recombinant phages to deliver antigens for vaccination against porcine cysticercosis. Thus, three peptides (KETc1, KETc12, GK1) and a recombinant antigen KETc7, previously proven to induce high levels of protection against pig cysticercosis, were expressed on the surface of the M13 bacteriophage at multiple copies. The pool of these four recombinant phages induced high levels of protection against an experimental murine cysticercosis. The immunogenicity of the phage vaccine preparation was therefore, tested in pigs, the natural host of Taenia solium. Subcutaneous or oral vaccination with these phages induced antigen-specific cellular immune responses in pigs. Preliminary data also points to the protective capacity of this recombinant phage vaccine against pig cysticercosis. The immunogenicity of these recombinant phages, together with the low cost of their production, make them a realistic candidate to be tested in pigs as an anti-cysticercus phage vaccine for field trials. This is the first report describing the application of a filamentous bacteriophage as a vaccine in large animals such as pigs, the only intermediate hosts of T. solium, a parasite of major medical importance in developing countries. The potential application of phages as a modern platform for vaccines for human and animal diseases is discussed.
Manoutcharian
Karen
K
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México CP 04510, D.F., Mexico. karman@servidor.unam.mx
Díaz-Orea
Alicia
A
Gevorkian
Goar
G
Fragoso
Gladis
G
Acero
Gonzalo
G
González
Erik
E
De Aluja
Aline
A
Villalobos
Nelly
N
Gómez-Conde
Eduardo
E
Sciutto
Edda
E
D000284:Administration, Oral; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000941:Antigens / Q000235:genetics / Q000276:immunology; D017104:Bacteriophage M13 / Q000235:genetics / Q000276:immunology*; D003551:Cysticercosis / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D005260:Female; D006651:Histocytochemistry; D007279:Injections, Subcutaneous / Q000662:veterinary; D008099:Liver / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000469:parasitology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000469:parasitology* / Q000517:prevention & control; D041201:Taenia solium / Q000276:immunology*; D014611:Vaccination / Q000379:methods / Q000662:veterinary*; D014614:Vaccines, Synthetic / Q000191:economics / Q000235:genetics / Q000276:immunology*
D000909:Antibodies, Helminth; D000941:Antigens; D000939:Epitopes; D014614:Vaccines, Synthetic