Structure of a highly phosphorylated O-polysaccharide of Proteus mirabilis O41.
A highly phosphorylated O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Proteus mirabilis O41 followed by GPC. The initial and dephosphorylated polysaccharides and phosphorylated products from two sequential Smith degradations were studied by (1)H, (13)C and (31)P NMR spectroscopy and ESI-MS. The O-polysaccharide was found to have a tetrasaccharide repeating unit containing one ribitol phosphate (presumably d-Rib-ol-5-P) and two ethanolamine phosphate (Etn-P) groups, one of which is present in the stoichiometric amount and the other in a nonstoichiometric amount. The following structure of the O-polysaccharide was established:
Senchenkova
Sof'ya N
SN
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
Perepelov
Andrei V
AV
Cedzynski
Maciej
M
Swierzko
Anna S
AS
Ziolkowski
Andrzej
A
Shashkov
Alexander S
AS
Kaca
Wieslaw
W
Knirel
Yuriy A
YA
Jansson
Per-Erik
PE
D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D010428:Pentosephosphates / Q000737:chemistry; D010766:Phosphorylation; D011513:Proteus mirabilis / Q000737:chemistry*
D019081:O Antigens; D010428:Pentosephosphates; C041025:ribitol-5-phosphate