The KlSRB10 gene from Kluyveromyces lactis.
We report the cloning and sequencing of a gene from Kluyveromyces lactis with high homology to the SRB10 gene (alias UME5, SSN3, GIG2, NUT7, RYE5) from Saccharomyces cerevisiae and other organisms. The KlSRB10 gene is located in a similar configuration to that found in S. cerevisiae, flanked by NOT4 and a gene with high similarity to YPL041c. The translated protein contains 593 amino acids and the characteristic domains of kinases from the CMGC subgroup. The functional relationship to yeast SRB10 is demonstrated by complementation of mutant phenotypes in a haploid S. cerevisiae strain containing a null allele.
Núñez
Laura
L
Departamento de Biología Celular y Molecular, Universidad de La Coruña, La Coruña 15071, Spain.
Fernández-Otero
Cristina
C
Rodríguez-Belmonte
Esther
E
Cerdán
M Esperanza
ME
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D056850:Cyclin-Dependent Kinase 8; D018844:Cyclin-Dependent Kinases / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D005816:Genetic Complementation Test; D007716:Kluyveromyces / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D005656:Fungal Proteins; D012331:RNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D056850:Cyclin-Dependent Kinase 8; D018844:Cyclin-Dependent Kinases; C498309:SSN3 protein, S cerevisiae