Antibiotic resistance, plasmid profile and RAPD-PCR analysis of enteropathogenic Escherichia coli (EPEC) clinical isolates.
Enteropathogenic Escherichia coli (EPEC) is a leading cause of diarrhea among infants in developing countries. A total of 38 EPEC isolates, obtained from diarrhea patients of Hospital Miri, Sarawak, were investigated through plasmid profile, antibiotic resistance and randomly amplified polymorphic DNA (RAPD) analysis. From the 8 types of antibiotics used, all isolates were 100% resistant to furoxime, cephalothin and sulphamethoxazole and showed high multiple antibiotic resistant (MAR) indexes, ranging from 0.5 to 1.0. In plasmid profiling, 22 isolates (58%) showed the presence of one or more plasmids in the range 1.0 to 30.9 mDa. The dendrogram obtained from the results of the RAPD-PCR discriminated the isolates into 30 single isolates and 3 clusters at the level of 40% similarity. The EPEC isolates were highly diverse, as shown by their differing plasmid profiles, antibiotic resistance patterns and RAPD profiles.
Wan
Keng Fei
KF
Department of Biotechnology, Faculty of Food Science and Biotechnology, University Putra Malaysia, UPM Serdang, Selangor, Malaysia.
Radu
Son
S
Cheah
Yoke Kqueen
YK
Benjamin
Patrick Guda
PG
Ling
Clemente Michael Wong Vui
CM
Hon
Shui Fong
SF
Harun
Abidah
A
Lo
Sze Khiong
SK
Liew
Phen Kiew
PK
D000328:Adult; D016172:DNA Fingerprinting / Q000379:methods*; D003967:Diarrhea / Q000382:microbiology; D004351:Drug Resistance / Q000235:genetics*; D004926:Escherichia coli / Q000145:classification* / Q000187:drug effects / Q000235:genetics; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D008296:Malaysia / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D011237:Predictive Value of Tests; D019105:Random Amplified Polymorphic DNA Technique