An electrochemical study of enzymatic oligonucleotide digestion.
This paper describes the synthesis and application of a novel ferrocene (Fc) label that can be efficiently attached to oligonucleotides. We demonstrate how pulse electrochemical methods can be used to measure very low concentrations of ferrocene label and, importantly, show good electroanalytical discrimination between a labelled oligonucleotide and an enzyme digested labelled oligonucleotide, in which the ferrocene label nucleotide conjugate has been released. Real time in situ analysis gives a much greater understanding of the process. Potential applications include the detection of specific nucleic acid sequences and measurement of nuclease activity.
Hillier
Stephen C
SC
Department of Chemistry, University of Bath, Bath BA2 7AY UK.
Frost
Christopher G
CG
Jenkins
A Toby A
AT
Braven
Helen T
HT
Keay
Russell W
RW
Flower
Stephen E
SE
Clarkson
John M
JM
D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D004563:Electrochemistry / Q000379:methods*; D005296:Ferrous Compounds / Q000737:chemistry*; D007700:Kinetics; D000075163:Metallocenes; D008832:Microchemistry / Q000379:methods*; D015335:Molecular Probes / Q000737:chemistry; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000032:analysis* / Q000737:chemistry*; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000032:analysis / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods
D005296:Ferrous Compounds; D000075163:Metallocenes; D015335:Molecular Probes; D009841:Oligonucleotides; D015719:Single-Strand Specific DNA and RNA Endonucleases; C004998:ferrocene