title: The study of the interaction of a model alpha-helical peptide with lipid bilayers and monolayers.
abstract: We studied the interaction of the alpha-helical peptide acetyl-Lys(2)-Leu(24)-Lys(2)-amide (L(24)) with tethered bilayer lipid membranes (tBLM) and lipid monolayers formed at an air-water interface. The interaction of L(24) with tBLM resulted in adsorption of the peptide to the surface of the bilayer, characterized by a binding constant K(c)=2.4+/-0.6 microM(-1). The peptide L(24) an induced decrease of the elasticity modulus of the tBLM in a direction perpendicular to the membrane surface, E(radial). The decrease of E(radial) with increasing peptide concentration can be connected with a disordering effect of the peptide to the tBLM structure. The pure peptide formed a stable monolayer at the air/water interface. The pressure-area isotherms were characterized by a transition of the peptide monolayer, which probably corresponds of the partial intercalation of the alpha-helixes at higher surface pressure. Interaction of the peptide molecules with lipid monolayers resulted in an increase of the mean molecular area of phospholipids both in the gel and liquid crystalline states. With increasing peptide concentration, the temperature of the phase transition of the monolayer shifted toward lower temperatures. The analysis showed that the peptide-lipid monolayer is not an ideally miscible system and that the peptide molecules form aggregates in the monolayer.
authors.affiliation: Department of Biophysics and Chemical Physics, Comenius University, Faculty of Mathematics and Physics, Mlynská dolina F1, 842 48 Bratislava, Slovakia.
authors.forename: P
authors.initials: P
authors.lastname: Vitovic
authors.forename: S
authors.initials: S
authors.lastname: Kresák
authors.forename: R
authors.initials: R
authors.lastname: Naumann
authors.forename: S M
authors.initials: SM
authors.lastname: Schiller
authors.forename: R N A H
authors.initials: RN
authors.lastname: Lewis
authors.forename: R N
authors.initials: RN
authors.lastname: McElhaney
authors.forename: T
authors.initials: T
authors.lastname: Hianik
mesh_terms: D000327:Adsorption; D040761:Biomimetic Materials / Q000737:chemistry*; D004548:Elasticity; D008051:Lipid Bilayers / Q000737:chemistry*; D008560:Membrane Fluidity; D008565:Membrane Proteins / Q000737:chemistry*; D008567:Membranes, Artificial; D010455:Peptides / Q000737:chemistry; D044367:Phase Transition; D010743:Phospholipids / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation
chemical_list: C419685:(LA)12 peptide; D008051:Lipid Bilayers; D008565:Membrane Proteins; D008567:Membranes, Artificial; D010455:Peptides; D010743:Phospholipids