title: Transmigration of blood leukocytes into the peritoneal cavity is related to the upregulation of ICAM-1 (CD54) and Mac-1 (CD11b/CD18) adhesion molecules.
abstract: BACKGROUND
  Migration of blood leukocytes into the peritoneal cavity of patients treated with peritoneal dialysis appears to be an important mechanism to prevent and fight peritonitis. To study the role of adhesion molecules in the process of leukocyte transmigration, we compared the expression of several adhesion receptors between peripheral blood monocytes and macrophages isolated from overnight dwell effluents.
  
  
  METHODS
  The study was performed in 12, stable, infection-free patients treated with continuous ambulatory peritoneal dialysis (CAPD) and in 9 patients during peritonitis. In another set of experiments, we analyzed the expression of these molecules on blood leukocytes in 10 predialysis chronic renal failure (CRF) patients and 9 healthy controls. Peritoneal cells from an 8-hour dwell were isolated by centrifugation. Expression of adhesion receptors CD11a, CD11b, CD18, CD49d, and CD54 on blood and peritoneal leukocytes was measured using flow cytometry.
  
  
  RESULTS
  In macrophages from the uninfected effluents, expression of both subunits of Mac-1 integrin receptor (CD11b and CD18) and intercellular adhesion molecule (ICAM)-1 receptor (CD54) was upregulated compared to peripheral blood monocytes from the same patients. The median value of mean fluorescence intensity in blood and effluent was 760.3 versus 1085.8 for CD11b (p = 0.013), 288.8 versus 448.6 for CD18 (p = 0.003), and 186.1 versus 365.7 for CD54 (p = 0.001). The same adhesion receptors were also significantly upregulated on peritoneal macrophages and neutrophils during peritonitis compared to blood leukocytes. Blood leukocytes from CAPD and CRF patients showed higher expression of CD54 and CD49d molecules compared to leukocytes from healthy controls.
  
  
  CONCLUSIONS
  These data suggest that transmigration of blood leukocytes into the peritoneal cavity during uncomplicated dialysis and in peritonitis is related to selective upregulation of ICAM-1 (CD54) and Mac-1 (CD18/CD11b) receptors.
authors.affiliation: Department of Nephrology, Transplantology and Internal Medicine, Gdańsk Medical University, Gdańsk, Poland. tlib@amg.gda.pl
authors.forename: Tomasz
authors.initials: T
authors.lastname: Liberek
authors.forename: Michal
authors.initials: M
authors.lastname: Chmielewski
authors.forename: Monika
authors.initials: M
authors.lastname: Lichodziejewska-Niemierko
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Lewandowski
authors.forename: Boleslaw
authors.initials: B
authors.lastname: Rutkowski
mesh_terms: D000328:Adult; D000368:Aged; D039481:CD11b Antigen / Q000378:metabolism*; D018821:CD18 Antigens / Q000378:metabolism*; D002634:Chemotaxis, Leukocyte / Q000502:physiology; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism*; D016177:Macrophage-1 Antigen / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010531:Peritoneal Dialysis, Continuous Ambulatory; D010537:Peritoneum / Q000276:immunology / Q000378:metabolism*; D010538:Peritonitis / Q000276:immunology / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D039481:CD11b Antigen; D018821:CD18 Antigens; D007136:Immunoglobulins; D016177:Macrophage-1 Antigen; D018799:Intercellular Adhesion Molecule-1