title: Residue depletion of tilmicosin in chicken tissues.
abstract: A high-performance liquid chromatography (HPLC) method with detection at 290 nm was modified and validated for the determination of tilmicosin residues in broiler chicken tissues. The limits of detection (LOD) of the method were 0.01 microg/g for muscle and 0.025 microg/g for liver and kidney. Average recoveries ranged from 80.4 to 88.3%. Relative standard deviation values ranged from 5.2 to 12.1%. Residue depletion of tilmicosin in broiler chickens was examined after dosing over a 5-day period by incorporation of the drug into drinking water at 37.5 and 75.0 mg/L. Tilmicosin concentrations in liver and kidney were highest on day 3 of medication and on day 5 in muscle, in both low- and high-dose groups. The residue levels in both groups were significantly higher in liver than in kidney or muscle. A minimum withdrawal time of 9 days was indicated for residue levels in muscle, liver, and kidney tissues below the maximum residue level (MRL).
authors.affiliation: Beijing Veterinary Diagnostic Institute, Beijing 100101, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Haiyang
authors.initials: H
authors.lastname: Jiang
authors.forename: Xingjun
authors.initials: X
authors.lastname: Jin
authors.forename: Zhangqi
authors.initials: Z
authors.lastname: Shen
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Shen
authors.forename: Caixia
authors.initials: C
authors.lastname: Fu
authors.forename: Junlin
authors.initials: J
authors.lastname: Guo
mesh_terms: D000818:Animals; D002645:Chickens; D004350:Drug Residues / Q000032:analysis*; D007668:Kidney / Q000737:chemistry; D007700:Kinetics; D008099:Liver / Q000737:chemistry; D018942:Macrolides / Q000008:administration & dosage / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000737:chemistry; D015645:Tylosin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000032:analysis*
chemical_list: D018942:Macrolides; C052319:tilmicosin; D015645:Tylosin