title: High-throughput preparative process utilizing three complementary chromatographic purification technologies.
abstract: A high-throughput process was developed in which wells in plates generated from parallel synthesis are automatically channeled to an appropriate purification technique using analytical data as a guide. Samples are directed to either of three fundamentally different preparative techniques: HPLC with UV-triggered fraction collection, supercritical fluid chromatography (SFC) with UV-triggered fraction collection, or HPLC with MS-triggered fraction collection. Automated analysis of the analytical data identifies the product compound mass and creates work lists based on chromatographic properties exhibited in the data so that each preparative instrument cherry picks the appropriate list of samples to purify when a preparative-scale plate is loaded.
authors.affiliation: Pfizer Global R&D-La Jolla, 10770 Science Center Drive, San Diego, CA 92121, USA. manuel.ventura@pfizer.com
authors.forename: Manuel
authors.initials: M
authors.lastname: Ventura
authors.forename: William
authors.initials: W
authors.lastname: Farrell
authors.forename: Christine
authors.initials: C
authors.lastname: Aurigemma
authors.forename: Kathleen
authors.initials: K
authors.lastname: Tivel
authors.forename: Michael
authors.initials: M
authors.lastname: Greig
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Wheatley
authors.forename: Alex
authors.initials: A
authors.lastname: Yanovsky
authors.forename: Kenneth Eric
authors.initials: KE
authors.lastname: Milgram
authors.forename: David
authors.initials: D
authors.lastname: Dalesandro
authors.forename: Raylyn
authors.initials: R
authors.lastname: DeGuzman
authors.forename: Phuong
authors.initials: P
authors.lastname: Tran
authors.forename: Leslie
authors.initials: L
authors.lastname: Nguyen
authors.forename: Loanne
authors.initials: L
authors.lastname: Chung
authors.forename: Ole
authors.initials: O
authors.lastname: Gron
authors.forename: Charles A
authors.initials: CA
authors.lastname: Koch
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet