Improved expression of novel red- and green-emitting luciferases of Phrixothrix railroad worms in mammalian cells.
Luciferases are widely used for the quantitative monitoring of gene expression in a variety of organisms. We successfully expressed novel red- and green-emitting luciferases of Phrixothrix railroad worms in mammalian cells in combination with the Kozak sequence and the CAG promoter. The characteristic properties of these luciferases indicate that they are appropriate reporter genes for the simultaneous monitoring of two gene expressions.
Nakajima
Yoshihiro
Y
Cell Dynamics Research Group, Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, Japan. y-nakajima@aist.go.jp
Kimura
Takuma
T
Suzuki
Chie
C
Ohmiya
Yoshihiro
Y
D000818:Animals; D002457:Cell Extracts; D003058:Cockroaches / Q000201:enzymology* / Q000235:genetics; D003116:Color; D015870:Gene Expression; D008156:Luciferases / Q000235:genetics* / Q000378:metabolism*; D049449:Luminescence; D051379:Mice; D041681:NIH 3T3 Cells; D013057:Spectrum Analysis
D002457:Cell Extracts; D008156:Luciferases