title: Improved expression of novel red- and green-emitting luciferases of Phrixothrix railroad worms in mammalian cells.
abstract: Luciferases are widely used for the quantitative monitoring of gene expression in a variety of organisms. We successfully expressed novel red- and green-emitting luciferases of Phrixothrix railroad worms in mammalian cells in combination with the Kozak sequence and the CAG promoter. The characteristic properties of these luciferases indicate that they are appropriate reporter genes for the simultaneous monitoring of two gene expressions.
authors.affiliation: Cell Dynamics Research Group, Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, Japan. y-nakajima@aist.go.jp
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nakajima
authors.forename: Takuma
authors.initials: T
authors.lastname: Kimura
authors.forename: Chie
authors.initials: C
authors.lastname: Suzuki
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ohmiya
mesh_terms: D000818:Animals; D002457:Cell Extracts; D003058:Cockroaches / Q000201:enzymology* / Q000235:genetics; D003116:Color; D015870:Gene Expression; D008156:Luciferases / Q000235:genetics* / Q000378:metabolism*; D049449:Luminescence; D051379:Mice; D041681:NIH 3T3 Cells; D013057:Spectrum Analysis
chemical_list: D002457:Cell Extracts; D008156:Luciferases