Mitochondrial complex I and IV activities in leukocytes from patients with parkin mutations.
The parkin protein functions as a RING-type ubiquitin protein ligase. Considering the possibility that impaired ubiquitin-proteosomal system activity may impair antioxidant defenses and enhance oxidative stress, we have investigated the activity of mitochondrial respiratory enzymes in patients with parkin gene mutations. A significant decrease in the leukocyte complex I activity was found both in patients with parkin mutations (62.5%) and idiopathic PD (64.5%) compared with age-matched controls (P < 0.001). Complex IV activity was also decreased significantly in idiopathic PD patients (60%), but no difference was detected between controls and patients with parkin mutations.
Müftüoglu
Meltem
M
Department of Biochemistry, Hacettepe University, Faculty of Medicine, Ankara, Turkey.
Elibol
Bülent
B
Dalmizrak
Ozlem
O
Ercan
Ayse
A
Kulaksiz
Gülnihal
G
Ogüs
Hamdi
H
Dalkara
Turgay
T
Ozer
Nazmi
N
D000328:Adult; D042967:Electron Transport Complex I / Q000235:genetics* / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000235:genetics* / Q000378:metabolism*; D005091:Exons / Q000235:genetics; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010300:Parkinson Disease / Q000235:genetics* / Q000378:metabolism*; D017354:Point Mutation / Q000235:genetics*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics* / Q000378:metabolism*
D003576:Electron Transport Complex IV; D044767:Ubiquitin-Protein Ligases; C111567:parkin protein; D042967:Electron Transport Complex I