title: Determination of potato glycoalkaloids using high-pressure liquid chromatography-electrospray ionisation/mass spectrometry.
abstract: A method for quantifying two toxic glycoalkaloids, alpha-solanine and alpha-chaconine, in potato (Solanum tuberosum) tuber tissue was developed using HPLC-electrospray ionisation (ESI)/MS. Potato samples were extracted with 5% aqueous acetic acid, and the extracts were subjected directly to HPLC-ESI/MS after filtration. By determining the intensities of the protonated molecules of alpha-solanine (m/z 868) and alpha-chaconine (m/z 852) using selected ion monitoring (positive ion mode), a sensitive assay was attained with detection limits of 38 and 14 ppb for the two glycoalkaloids, respectively. The high sensitivity and selectivity of MS detection effectively reduced the time of analysis thus enabling a high throughput assay of glycoalkaloids in potato tubers.
authors.affiliation: Division of Applied Life Sciences, Department of Agriculture, Kyoto University, Kyoto, 606-8502 Japan. matsuda@kais.kyoto-u.ac.jp
authors.forename: Fumio
authors.initials: F
authors.lastname: Matsuda
authors.forename: Keiko
authors.initials: K
authors.lastname: Morino
authors.forename: Haruna
authors.initials: H
authors.lastname: Miyazawa
authors.forename: Masahiro
authors.initials: M
authors.lastname: Miyashita
authors.forename: Hisashi
authors.initials: H
authors.lastname: Miyagawa
mesh_terms: D002236:Carbohydrate Conformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012992:Solanine / Q000031:analogs & derivatives* / Q000032:analysis*; D011198:Solanum tuberosum / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D012992:Solanine; C011860:alpha-chaconine