title: EtCRK2, a cyclin-dependent kinase gene expressed during the sexual and asexual phases of the Eimeria tenella life cycle.
abstract: EtCRK2, a cyclin-dependent kinase from the coccidian parasite, Eimeria tenella is closely related to eukaryotic cyclin-dependent kinases that regulate progression of the cell cycle and to several cyclin-dependent kinases identified in the Apicomplexa. Northern blot analyses revealed that EtCRK2 is transcribed during both asexual (first-generation schizogony) and sexual (oocyst sporulation) replicative phases of the parasite life cycle. In addition, it appears to be transcriptionally regulated during meiosis. Recombinant EtCRK2 produced in Escherichia coli has kinase activity which is significantly stimulated by the addition of vertebrate cyclin A. This cyclin-dependent kinase may play a significant role in regulating critical cell cycle events during both asexual proliferation and sexual development of the parasite.
authors.affiliation: Veterinary Parasitology, Institute of Comparative Medicine, University of Glasgow, Garscube Estate, Bearsden Road, Glasgow G61 1QH, UK. j.kinnaird@vet.gla.ac.uk
authors.forename: J H
authors.initials: JH
authors.lastname: Kinnaird
authors.forename: J M
authors.initials: JM
authors.lastname: Bumstead
authors.forename: D J
authors.initials: DJ
authors.lastname: Mann
authors.forename: R
authors.initials: R
authors.lastname: Ryan
authors.forename: M W
authors.initials: MW
authors.lastname: Shirley
authors.forename: B R
authors.initials: BR
authors.lastname: Shiels
authors.forename: F M
authors.initials: FM
authors.lastname: Tomley
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern / Q000379:methods; D019925:Cyclin A / Q000378:metabolism; D016786:Eimeria tenella / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D008018:Life Cycle Stages / Q000235:genetics*; D008540:Meiosis / Q000235:genetics; D008969:Molecular Sequence Data; D033661:Oocysts / Q000235:genetics; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D050737:Proto-Oncogene Proteins c-crk; D011994:Recombinant Proteins / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D019925:Cyclin A; D011518:Proto-Oncogene Proteins; D050737:Proto-Oncogene Proteins c-crk; D011994:Recombinant Proteins; D011505:Protein-Tyrosine Kinases