title: Spatiotemporal quantification of cerebral blood flow during functional activation in rat somatosensory cortex using laser-speckle flowmetry.
abstract: Laser-speckle flowmetry was used to characterize activation flow coupling after electrical somatosensory stimulation of forepaw and hindpaw in the rat. Quantification of functional activation was made with high transverse spatial (microm) and temporal (msec) resolution. Different activation levels and duration of stimulation were quantitatively investigated, and were in good agreement with previous laser-Doppler measurements. Interestingly, the magnitude but not the overall shape of the response was found to scale with stimulus amplitude and the distance from the activation centroid. The results provide new insights about the spatial characteristics of cerebral blood flow response to functional activation, and the method should lead to improved understanding of the coupling of neuronal activity and hemodynamics under normal and pathologic conditions.
authors.affiliation: Departments of Physics and Astronomy, University of Pennsylvania, Philadelphia, PA 19104, USA. durduran@stwing.upenn.edu
authors.forename: Turgut
authors.initials: T
authors.lastname: Durduran
authors.forename: Mark G
authors.initials: MG
authors.lastname: Burnett
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Yu
authors.forename: Chao
authors.initials: C
authors.lastname: Zhou
authors.forename: Daisuke
authors.initials: D
authors.lastname: Furuya
authors.forename: Arjun G
authors.initials: AG
authors.lastname: Yodh
authors.forename: John A
authors.initials: JA
authors.lastname: Detre
authors.forename: Joel H
authors.initials: JH
authors.lastname: Greenberg
mesh_terms: D000818:Animals; D002560:Cerebrovascular Circulation; D004558:Electric Stimulation; D005528:Foot; D017078:Laser-Doppler Flowmetry / Q000295:instrumentation / Q000379:methods*; D008297:Male; D008962:Models, Theoretical; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow; D013003:Somatosensory Cortex / Q000033:anatomy & histology / Q000502:physiology*