title: Immunohistochemical localization of integrin alpha V beta 3 and osteopontin suggests that they do not interact during embryo implantation in ruminants.
abstract: BACKGROUND
  It has been suggested that trophoblast attachment requires co-expression of integrin alpha V beta 3 and its ligand osteopontin at the fetal-maternal interface. Until now the expression patterns of integrin alpha V beta 3 and osteopontin in the pregnant bovine uterus were unknown. The objectives of this study were to localize integrin alpha V beta 3 and osteopontin in bovine and sheep endometrium during the periimplantation period and to compare the distribution patterns using antibodies that had not yet been tested in sheep.
  
  
  METHODS
  Cell compartments within endometrial tissue sections were scored for immunohistochemical staining intensity and data were analyzed to determine the effects of day of pregnancy or cycle.
  
  
  RESULTS
  In pregnant bovine endometrium, integrin alpha V beta 3 was detected in luminal epithelium, stroma, myometrium and smooth muscle. A strong band of immunoreactivity was observed in the subepithelial stroma of intercaruncular regions, but there was reduced reactivity in the caruncles and glands. Bovine trophoblast did not express integrin alpha V beta 3 at any stage of pregnancy. In ovine endometrium a different pattern of staining for integrin alpha V beta 3 was observed. Reactivity was not present in the luminal epithelium or trophoblast. There was strong staining of the deep glands and no reactivity in the superficial glands. Osteopontin distribution was similar for sheep and cattle. For both species, apical staining was present on the luminal epithelium and glands and on embryonic tissues.
  
  
  CONCLUSIONS
  In ruminants, integrin alpha V beta 3 and osteopontin do not co-localize at the fetal-maternal interface indicating that these proteins could not interact to facilitate embryo attachment as has been proposed in other species.
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS-INSERM-Université Louis Pasteur, B.P. 16367404 Illkirch, Strasbourg, France. sarahk@igbmc.u-strasbg.fr
authors.forename: Sarah
authors.initials: S
authors.lastname: Kimmins
authors.forename: Hai Choo
authors.initials: HC
authors.lastname: Lim
authors.forename: Leslie A
authors.initials: LA
authors.lastname: MacLaren
mesh_terms: D000818:Animals; D002417:Cattle; D010064:Embryo Implantation / Q000502:physiology*; D004717:Endometrium / Q000737:chemistry; D004848:Epithelium / Q000737:chemistry; D030762:Estrous Cycle; D005260:Female; D007150:Immunohistochemistry; D039302:Integrin alphaVbeta3 / Q000032:analysis* / Q000378:metabolism; D008297:Male; D053495:Osteopontin; D011247:Pregnancy; D011485:Protein Binding; D012756:Sheep; D012795:Sialoglycoproteins / Q000032:analysis* / Q000378:metabolism
chemical_list: D039302:Integrin alphaVbeta3; D012795:Sialoglycoproteins; D053495:Osteopontin