Simultaneous determination of the major isosteroidal alkaloids and their glucosides in the bulbs of Fritillaria by high performance liquid chromatography coupled with evaporative light scattering detection.
OBJECTIVE
To establish an HPLC-ELSD method for the simultaneous determination of five major bioactive isosteroidal alkaloids and gluco-alkaloids in the bulbs of Fritillaria namely peimissine, imperialine, sinpeinine A, imperialine-3 beta-glucoside and yibeinoside A.


METHODS
A Nova-Pak C18 column (150 mm x 3.9 mm ID) was used. The chromatography was carried out with a linear gradient programming. The mobile phase was acetonitrile-water (containing 0.1% diethylamine) and the flow rate was 1.0 mL.min-1.


RESULTS
The linear range of peimissine was 13.1-288.2 mg.L-1 (r2 = 0.9975), imperialine-3 beta-glucoside 7.7-169.4 mg.L-1 (r2 = 0.9993), yibeinoside A 7.3-160.6 mg.L-1 (r2 = 0.9997), imperialine 16.5-363.0 mg.L-1 (r2 = 0.9992), sinpeinine A 8.7-191.4 mg.L-1 (r2 = 0.9942).


CONCLUSIONS
The method is accurate with overall intra- and inter-day variation less than 5% and recovery more than 95%. The method was successfully applied to analyze five major bioactive alkaloids and gluco-alkaloids in three Fritillaria bulbs.
Li
Ping
P
Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210009, China. lipingli@public1.ptt.js.cn
Zeng
Ling-jie
LJ
Li
Song-lin
SL
Bi
Zhi-ming
ZM
Lin
Ge
G
D000470:Alkaloids / Q000032:analysis*; D002595:Cevanes / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D031410:Fritillaria / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*
D000470:Alkaloids; D002595:Cevanes; D004365:Drugs, Chinese Herbal; D005960:Glucosides; C052510:peimisine; C069537:sinpeinine A; C069538:yibeinoside A; C047331:peiminine