[Determination of aloin in aloes by HPLC].
OBJECTIVE
To describe a simple and rapid isocratic reversed-phase high performance liquid chromatography method for the baseline separation, identification and assay of aloin in aloes.


METHODS
The analytical column was a ZORBAX SB-C18(4.6 mm x 250 mm) filled with a 5 microns stationary phase. The mobile phase consisted of acetonitrile-water (25:75); the flow-rate was 1 mL.min-1. The injection volume was 10 microL. The DAD detector was set at 355 nm.


RESULTS
The calibration curve was linear over the range of 0.17-5.9 micrograms (r = 0.9999). The average recovery of the method was 98.6%, RSD 1.32% (n = 6).


CONCLUSIONS
The results showed that this method was reliable and accurate. The method was applied to eleven Cape and East African aloes of different origin.
Cao
Hong
H
Institute for Drug and Instrument Control of PLA, Beijing 100071, China.
Liu
Yun
Y
D000504:Aloe / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004642:Emodin / Q000031:analogs & derivatives / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*
D004642:Emodin; C006457:alloin