Real-time scanning tunnelling microscopy imaging of protein motion at electrode surfaces.
A mutant (K27C) of the blue copper protein azurin [Eur. J. Biochem. 194 (1990) 109; J. Mol. Biol. 221 (1991) 765] for orientated immobilisation on gold surfaces was analysed by scanning tunnelling microscopy (STM) both in a resting state and following the application of a short potential pulse between the tip and sample.
Halliwell
C M
CM
National Physical Laboratory, Queens Road, Teddington, Middlesex, TW11 0LW, UK. Catherine.Halliwell@npl.co.uk
Davies
J A
JA
Gallop
J C
JC
Josephs-Franks
P W
PW
D000327:Adsorption; D001400:Azurin / Q000032:analysis / Q000737:chemistry / Q000648:ultrastructure*; D015374:Biosensing Techniques / Q000295:instrumentation*; D020099:Coated Materials, Biocompatible / Q000032:analysis / Q000737:chemistry; D019281:Dimerization; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D019544:Equipment Failure Analysis / Q000379:methods*; D008422:Materials Testing; D016252:Microscopy, Scanning Tunneling / Q000379:methods*; D009038:Motion; D009862:Online Systems; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D001400:Azurin