Rapid and sensitive detection of 17beta-estradiol in environmental water using automated immunoassay system with bacterial magnetic particles.
A fully automated immunoassay of 17beta-estradiol (E2) was performed using anti-E2 monoclonal antibody immobilized on bacterial magnetic particles (AntiE2-BMPs) and alkaline phosphatase-conjugated E2 (ALP-E2). E2 concentration in environmental water samples was evaluated by decrease in luminescence based on competitive reaction. A linear correlation between the luminescence intensity and E2 concentration was obtained between 0.5 and 5 ppb. The minimum detectable concentration of E2 was 20 ppt. All measurement steps were done within 0.5 h. The analysis of environmental water samples by a commercially available ELISA kit and the BMP-based immunoassay gave good correlation plots with a correlation efficient of 0.992. These results suggest that the fully automated system using the BMP-based immunoassay has some advantages in the high rapidity and sensitivity of the measurement. This system will enable us to determine low E2 concentrations without sample condensation.
Tanaka
Tsuyoshi
T
Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Naka-Cho, Koganei, Tokyo 184-8588, Japan.
Takeda
Hajime
H
Ueki
Fumiko
F
Obata
Kimimichi
K
Tajima
Hideji
H
Takeyama
Haruko
H
Goda
Yasuhiro
Y
Fujimoto
Shigeru
S
Matsunaga
Tadashi
T
D001426:Bacterial Proteins / Q000276:immunology*; D004784:Environmental Monitoring / Q000379:methods*; D004958:Estradiol / Q000032:analysis* / Q000276:immunology*; D007124:Immunoenzyme Techniques / Q000379:methods*; D008565:Membrane Proteins / Q000276:immunology*; D015203:Reproducibility of Results; D012371:Robotics / Q000379:methods; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D014874:Water Pollutants, Chemical / Q000032:analysis*
D001426:Bacterial Proteins; D008565:Membrane Proteins; C405425:MpsA protein, Magnetospirillum sp.; D014874:Water Pollutants, Chemical; D004958:Estradiol