Moderate skin sensitizers can induce phenotypic changes on in vitro generated dendritic cells.
In the present study, we analyzed the phenotypic alterations induced by several allergens on immature dendritic cells (DC), with the aim to develop a potential in vitro alternative for predicting the sensitizing potential of chemicals. DC were generated from human monocytes cultured in the presence of GM-CSF, IL-4 and TGF-beta1 and treated for 2 or 4 days with different chemicals. Surface marker expression (HLA-DR, CD1a, CD40, CD54, CD83, CD86, CCR7 and E-cadherin) was analyzed by flow cytometry. Results showed that a 2-day treatment with the representative allergens DNCB and NiSO(4) induced significant changes of most antigens while other chemicals such as balm of Peru (strong allergen), kathon (moderate allergen), cinnamic aldehyde (mild allergen) or the irritant SLS had no significant effect. In contrast, the 4-day treatment with allergens substantially improved the results. Indeed, despite a large variability according to the donors, the number of modified antigens was significantly higher with all the tested chemicals, except kathon, as compared to that observed with the irritant SLS. The present study indicates that, in this model, the screening of mild or moderate allergens requires both the consideration of many antigens and a prolonged time of incubation with the chemicals.
Staquet
M J
MJ
INSERM U 346, Pavillon R, Hôpital E. Herriot, 69437, Lyon 03, France.
Sportouch
M
M
Jacquet
C
C
Schmitt
D
D
Guesnet
J
J
Péguet-Navarro
J
J
D000485:Allergens / Q000009:adverse effects*; D000954:Antigens, Surface / Q000032:analysis*; D018929:Cell Culture Techniques; D016207:Cytokines / Q000494:pharmacology; D003713:Dendritic Cells / Q000502:physiology*; D004353:Drug Evaluation, Preclinical; D005544:Forecasting; D006801:Humans; D007114:Immunization; D009000:Monocytes / Q000276:immunology; D010641:Phenotype
D000485:Allergens; D000954:Antigens, Surface; D016207:Cytokines