Effects of annexins II and V on survival of neurons and astrocytes in vitro.
OBJECTIVE
To study the effects of annexins II and V on the survival and neurite outgrowth of primary cultured neurons and the survival of astrocytes after peroxide and hypoxia insults in vitro.


METHODS
Annexins II and V proteins and/or corresponding antibodies were added to the medium of primary neocortical cultures. H(2)O(2) and NaN(3) were used to induce neuron injury, respectively. Lactate dehydrogenase (LDH) release was measured.


RESULTS
Addition of annexin II or V into the culture medium did not affect the normal survival and neurite outgrowth of cortical neurons. However, when an antibody against annexin II or V was added to the culture, the survival and neurite outgrowth of these neurons markedly declined. Further, addition of the two annexins into cortical cultures after peroxide and hypoxia insults markedly reduced the LDH release and cell death.


CONCLUSIONS
Annexins II and V are essential for the survival and neurite outgrowth of developing cortical neurons, the survival of glial cells, and protect neurons and glial cells against peroxide and hypoxia injuries.
Han
Shu
S
Department of Neurobiology, Shanghai Second Medical University, Shanghai 200025, China.
Zhang
Kai-hua
KH
Lu
Pei-hua
PH
Xu
Xiao-ming
XM
D000818:Animals; D017306:Annexin A2 / Q000276:immunology / Q000494:pharmacology*; D017304:Annexin A5 / Q000276:immunology / Q000494:pharmacology*; D000906:Antibodies / Q000494:pharmacology; D001253:Astrocytes / Q000187:drug effects*; D015687:Cell Hypoxia; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D004622:Embryo, Mammalian; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D016501:Neurites / Q000187:drug effects; D009474:Neurons / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D017306:Annexin A2; D017304:Annexin A5; D000906:Antibodies; D018696:Neuroprotective Agents; D007770:L-Lactate Dehydrogenase