[Effect of overall alkali of Tongbiling on CD69 expression activated mouse T lymphocytes].
OBJECTIVE
To investigate the effect of overall alkali of Tongbiling(TBL) on CD69 expression on activated mouse T lymphocytes and its possible mechanism.


METHODS
Phorbol 12,13-dibutyrate(PDB) or concanavalin (ConA) were added successively into mouse lymphocytic culture with various concentration of overall alkali TBL. After 24 hours, CD69 expression rate on mouse T lymphocytes activated with PDB or ConA was analyzed by flow cytometry.


RESULTS
Overall alkali TBL could significantly down-regulate CD69 expression in a dose-dependent manner.


CONCLUSIONS
Overall alkali TBL can significantly inhibit CD69 expression on activated mouse T lymphocytes. This study provided an experimental basis for application of overall alkali TBL to treatment of rheumatoid arthritis.
Chen
Ji-fan
JF
The First Attached Hospital, Guangzhou TCM University, Guangzhou 510405, China. guangxing_chen@hotmail.com
Chen
Guang-xing
GX
Li
Xiao-juan
XJ
Quan
Shi-ming
SM
Zeng
Yao-ying
YY
Hu
Ying-je
YJ
Huang
Ke-er
KE
Liu
Xiao-ling
XL
D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D031403:Anemarrhena / Q000737:chemistry; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism*; D002935:Cinnamomum zeylanicum / Q000737:chemistry; D015536:Down-Regulation; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D037181:Lectins, C-Type; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D029596:Paeonia / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D000099192:CD69 Antigens
D000470:Alkaloids; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D037181:Lectins, C-Type; D000099192:CD69 Antigens