title: Activation of crystalline cellulose surfaces through the chemoenzymatic modification of xyloglucan.
abstract: Cellulose constitutes an important raw material for many industries. However, the superb load-bearing properties of cellulose are accompanied by poor chemical reactivity. The hydroxyl groups on cellulose surfaces can be reacted but usually not without loss of fiber integrity and strength. Here, we describe a novel chemoenzymatic approach for the efficient incorporation of chemical functionality onto cellulose surfaces. The modification is brought about by using a transglycosylating enzyme, xyloglucan endotranglycosylase, to join chemically modified xyloglucan oligosaccharides to xyloglucan, which has a naturally high affinity to cellulose. Binding of the chemically modified hemicellulose molecules can thus be used to attach a wide variety of chemical moieties without disruption of the individual fiber or fiber matrix.
authors.affiliation: Department of Biotechnology, Royal Institute of Technology, AlbaNova University Center, SE-106 91 Stockholm, Sweden.
authors.forename: Harry
authors.initials: H
authors.lastname: Brumer
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Baumann
authors.forename: Kjell
authors.initials: K
authors.lastname: Carlsson
authors.forename: Tuula T
authors.initials: TT
authors.lastname: Teeri
mesh_terms: D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism*; D003460:Crystallization; D016650:Fluorescein-5-isothiocyanate; D005936:Glucans / Q000737:chemistry* / Q000378:metabolism*; D016695:Glycosyltransferases / Q000378:metabolism; D018613:Microscopy, Confocal; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties; D014990:Xylans / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D005936:Glucans; D009844:Oligosaccharides; D014990:Xylans; C029353:xyloglucan; D002482:Cellulose; D016695:Glycosyltransferases; C073693:xyloglucan endotransglycosylase; D016650:Fluorescein-5-isothiocyanate