title: Reversible immobilization of engineered molecules by Ni-NTA chelators.
abstract: Electrochemical synthesis of nickel-nitrilotriacetic acid (Ni-NTA) chelators, for subsequent immobilization of (His)(6)-tagged proteins (Photosystem II (PSII) as model molecule), on Au or Au-graphite electrodes is compared to chemical synthesis. Results show: (i) higher Ni-NTA surface density, (ii) shorter treatment time (1-12 min vs. 16 h normally needed for self-assembled monolayer (SAM)), (iii) possibility of addressing the chelator to only one Au electrode, in a sensor micro-array.
authors.affiliation: Department of Biology, University of J.E. Purkyne, Usti nad Labem, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Maly
authors.forename: C
authors.initials: C
authors.lastname: Di Meo
authors.forename: M
authors.initials: M
authors.lastname: De Francesco
authors.forename: A
authors.initials: A
authors.lastname: Masci
authors.forename: J
authors.initials: J
authors.lastname: Masojidek
authors.forename: M
authors.initials: M
authors.lastname: Sugiura
authors.forename: A
authors.initials: A
authors.lastname: Volpe
authors.forename: R
authors.initials: R
authors.lastname: Pilloton
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000379:methods*; D002614:Chelating Agents / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D008027:Light; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives* / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D045332:Photosystem II Protein Complex / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics / Q000528:radiation effects; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000737:chemistry / Q000528:radiation effects
chemical_list: D002614:Chelating Agents; D020099:Coated Materials, Biocompatible; D009942:Organometallic Compounds; D045332:Photosystem II Protein Complex; D011994:Recombinant Proteins; C088321:nickel nitrilotriacetic acid; D009571:Nitrilotriacetic Acid