Modelling effect of physical and chemical parameters on heat inactivation kinetics of hepatitis A virus in a fruit model system.
While thermal destruction of pathogenic bacteria has been thoroughly studied in food industry, heat inactivation of viruses in food has been poorly investigated. Experiments were carried out to characterize the effects of controlled physical and chemical characteristics of a food matrix upon heat resistance parameters (D and z values) of hepatitis A virus (HAV), taken as model because of its reported heat resistance. Sucrose content (28-52 degrees Brix), calcium concentration (90-1700 mg kg(-1)) and pH (3.3-4.3) were selected for possible influence on thermal inactivation of HAV in strawberry mashes and thus included in an experimental design according to a Doehlert matrix. Use of this design not only allowed to detect and quantify the direct influence of sucrose concentration upon the D85 degrees C value to be higher than the one of pH, but also to reveal a sucrose concentration/pH specific interaction, while no effect of calcium concentration was evidenced. Although the model cannot be directly used to predict heat resistance in real fruit systems, because of differences observed between predicted and measured D85 degrees C values, it is useful for predicting the trends and relative changes in D values due to sucrose concentration and pH variations. Results suggested possible effects of other constituents of strawberry products on heat resistance of HAV and confirmed the importance of experimental validation of any model-derived process. Nevertheless, such a modelling approach using response surface methodology provides a rapid answer to heat resistance evaluation of a food-borne virus as a function of specific physical and chemical parameters of specific food products.
Deboosere
N
N
Unité de Sécurité Microbiologique, Institut Pasteur de Lille, 1 rue du Professeur Calmette, BP 245, 59019 Lille, France. nathalie.deboosere@pasteur-lille.fr
Legeay
O
O
Caudrelier
Y
Y
Lange
M
M
D002118:Calcium / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005516:Food Microbiology; D031985:Fragaria / Q000821:virology*; D030041:Hepatitis A virus / Q000187:drug effects / Q000254:growth & development / Q000472:pathogenicity*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D011237:Predictive Value of Tests; D013395:Sucrose / Q000494:pharmacology*
D013395:Sucrose; D002118:Calcium