[The first pregnancy and birth after cryopreservation of oocytes in the Czech Republic--importance of the method for reproductive medicine].
OBJECTIVE
The purpose of this work was to develop methods for successful cryopreservation of human oocytes.


METHODS
Case-controlled study and case report.


METHODS
Sanatorium Pronatal, Prague.


METHODS
Propanediol (PrOH)-sucrose was used as cryoprotectant medium for cumulus-free oocytes and stepwise dilution of cryoprotectant post-thaw.


RESULTS
The method was used in three patients (38 denuded oocytes) and yielded excellent survival and fertilization rates (89.5% and 73.5% respectively). In all patients embryo-transfer was possible, one patient got pregnant and delivered a healthy baby.


CONCLUSIONS
Our data show that cryopreservation may ensure that the integrity of the human oocyte is adequate for normal fertilization and embryo development.
Huttelová
R
R
Sanatorium Pronatal, Praha.
Mardesić
T
T
Becvárová
V
V
Brachtlová
T
T
D000328:Adult; D015925:Cryopreservation; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007248:Infertility, Male / Q000628:therapy; D008297:Male; D009865:Oocytes; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic