title: Gamma-H2AX expression pattern in non-irradiated neonatal mouse germ cells and after low-dose gamma-radiation: relationships between chromatid breaks and DNA double-strand breaks.
abstract: The DNA double-strand breaks (DSBs) are considered to be the most relevant lesions for the deleterious effects of ionizing radiation exposure. The discovery that the induction of DSBs is rapidly followed by the phosphorylation of H2AX histone at Ser-139, favoring repair protein recruitment or access, opens the possibility for a wide range of research. This phosphorylated histone, named gamma-H2AX, has been shown to form foci in interphase nuclei as well as megabase chromatin domains surrounding the DNA lesion on chromosomes. Using detection of gamma-H2AX on germ cell mitotic chromosomes 2 h after gamma-irradiation, we studied radiation-induced DSBs during the G(2)/M phase of the cell cycle. We show that 1) non-irradiated neonatal germ cells express gamma-H2AX with variable patterns at metaphase, 2) gamma-irradiation induces foci whose number increases in a dose-dependent manner, 3) some foci correspond to visible chromatid breaks or exchanges, 4) sticky chromosomes characterizing cell radiation exposure during mitosis are a consequence of DSBs, and 5) gamma-H2AX remains localized at the sites of the lesions even after end-joining has taken place. This suggests that completion of DSB repair does not necessarily imply disappearance of gamma-H2AX.
authors.affiliation: INSERM U566-CEA-Paris 7, Laboratoire de radiosensibilité des cellules germinales, Département de Radiobiologie et Radiopathologie, Direction des sciences du vivant, Commissariat à l'Energie Atomique, BP 6, 92265 Fontenay-aux-Roses Cedex, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Forand
authors.forename: Bernard
authors.initials: B
authors.lastname: Dutrillaux
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Bernardino-Sgherri
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001399:Azure Stains; D002842:Chromatids / Q000528:radiation effects*; D002869:Chromosome Aberrations; D004247:DNA / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D015870:Gene Expression / Q000528:radiation effects; D006657:Histones / Q000235:genetics*; D008297:Male; D008677:Metaphase; D051379:Mice; D008815:Mice, Inbred Strains; D012854:Sister Chromatid Exchange / Q000528:radiation effects; D013091:Spermatogenesis / Q000235:genetics / Q000528:radiation effects; D013093:Spermatogonia / Q000502:physiology / Q000528:radiation effects*
chemical_list: D001399:Azure Stains; D006657:Histones; C475961:gamma-H2AX protein, mouse; D004247:DNA