title: Transformation of folate-consuming Lactobacillus gasseri into a folate producer.
abstract: Five genes essential for folate biosynthesis in Lactococcus lactis were cloned on a broad-host-range lactococcal vector and were transferred to the folate auxotroph Lactobacillus gasseri. As a result L. gasseri changed from a folate consumer to a folate producer. This principle can be used to increase folate levels in many fermented food products.
authors.affiliation: Wageningen Centre for Food Sciences and NIZO food research, Ede, The Netherlands.
authors.forename: Arno
authors.initials: A
authors.lastname: Wegkamp
authors.forename: Marjo
authors.initials: M
authors.lastname: Starrenburg
authors.forename: Willem M
authors.initials: WM
authors.lastname: de Vos
authors.forename: Jeroen
authors.initials: J
authors.lastname: Hugenholtz
authors.forename: Wilbert
authors.initials: W
authors.lastname: Sybesma
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005492:Folic Acid / Q000096:biosynthesis* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D007778:Lactobacillus / Q000235:genetics / Q000378:metabolism*; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family; D010957:Plasmids; D014169:Transformation, Bacterial
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005492:Folic Acid