title: Square wave and elimination voltammetric analysis of azidothymidine in the presence of oligonucleotides and chromosomal DNA.
abstract: Azidothymidine (AZT, 3'-azido-3'-deoxythymidine, Zidovudine, Retrovir) is an approved and widely used antiretroviral drug for the treatment of human immunodeficiency virus (HIV) infection. Dynamic electrochemical methods have been employed for the fast and inexpensive determination of this drug in natural samples. The electrochemical signal of AZT, resulting from the reduction of azido group, was studied by square wave voltammetry (SWV), linear sweep voltammetry (LSV) and elimination voltammetry with linear scan (EVLS) using a hanging mercury drop electrode (HMDE). This paper explores the possibility of determining AZT in the presence of native (dsDNA) or denatured calf thymus DNA (ssDNA), and/or some synthetic oligodeoxynucleotides (ODNs). The detection limit of AZT in the absence and in the presence of ssDNA (10 microg/ml) is 1 and 250 nM, respectively. It was found that the signal of AZT is not substantially affected by the presence of DNA. We can therefore assume that the electrons are transferred through the adsorption layer of nucleic acids. By using the elimination procedure, both irreversible reduction signals of AZT and DNA are augmented. Moreover, the elimination signal in the peak-counterpeak form may indicate the adsorption of the analytes on the electrode surface preceding an electron transfer.
authors.affiliation: Department of Theoretical and Physical Chemistry, Faculty of Science, Masaryk University Brno, Kotlárská 2, 611 37 Brno, Czech Republic. libuse@chemi.muni.cz
authors.forename: Libuse
authors.initials: L
authors.lastname: Trnková
authors.forename: René
authors.initials: R
authors.lastname: Kizek
authors.forename: Jan
authors.initials: J
authors.lastname: Vacek
mesh_terms: D002875:Chromosomes / Q000737:chemistry; D045424:Complex Mixtures / Q000032:analysis* / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods*; D009841:Oligonucleotides / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D015215:Zidovudine / Q000032:analysis* / Q000737:chemistry
chemical_list: D045424:Complex Mixtures; D009841:Oligonucleotides; D015215:Zidovudine; D004247:DNA; C427203:calf thymus DNA