title: Vitronectin is significant in the adhesion of lens epithelial cells to PMMA polymers.
abstract: A major complication of intraocular lens surgery is diminished visual acuity caused by the regrowth of lens epithelial cells (secondary cataract). Polymethylmethacrylate (PMMA) is a commonly used intraocular lens material. This study addresses the mechanisms underlying the initial adhesion of lens epithelial cells to PMMA and a functionalized PMMA-based terpolymer known to inhibit cell proliferation. Rabbit lens epithelial cells were cultured on the test polymer surfaces in medium containing serum depleted of either fibronectin or vitronectin (or both) to identify the role of these proteins in the initial process of cell adhesion. Adherent cells were quantitated after 60 min, and the actin cytoskeleton and focal contact formation were compared in each serum treatment on both polymers. Vitronectin was significantly more effective for initial cell attachment to both polymers than fibronectin. Normal cell spreading on PMMA required vitronectin and was independent of fibronectin, whereas cell spreading on the terpolymer was abnormal and required the presence of fibronectin and vitronectin together. Together, these results help to explain the inhibition of cell proliferation previously shown on the functionalized PMMA. This work contributes to the design of a polymer for use in intraocular lenses that inhibits proliferation of the target cells.
authors.affiliation: CSIRO Molecular Science, 11 Julius Avenue, North Ryde, Sydney, NSW, Australia 2113. meg.evans@csiro.au
authors.forename: Margaret D M
authors.initials: MD
authors.lastname: Evans
authors.forename: Graciela
authors.initials: G
authors.lastname: Pavon-Djavid
authors.forename: Gérard
authors.initials: G
authors.lastname: Hélary
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Legeais
authors.forename: Véronique
authors.initials: V
authors.lastname: Migonney
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology*; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D022001:Focal Adhesions / Q000378:metabolism; D007908:Lens, Crystalline / Q000166:cytology*; D008422:Materials Testing; D019904:Polymethyl Methacrylate / Q000737:chemistry / Q000378:metabolism*; D019736:Prostheses and Implants; D011817:Rabbits; D044967:Serum / Q000737:chemistry; D013499:Surface Properties; D019096:Vitronectin / Q000378:metabolism*
chemical_list: D001672:Biocompatible Materials; D019096:Vitronectin; D019904:Polymethyl Methacrylate