Simultaneous quantification of beclomethasone dipropionate and its metabolite, beclomethasone 17-monopropionate in rat and human plasma and different rat tissues by liquid chromatography-positive electrospray ionization tandem mass spectrometry.
A sensitive, rapid and selective liquid chromatography-positive electrospray ionization tandem mass spectrometry (LC-(ESI+)-MS-MS) method has been developed and validated for the simultaneous quantification of beclomethasone dipropionate (BDP) and its active metabolite, beclomethasone 17-monopropionate (17-BMP) in rat plasma and different tissues using fluticasone propionate (FP) as the internal standard. The method was validated over a linear range from 0.05 to 5 ng/ml for both analytes. A solid-phase extraction procedure was used for plasma samples and a liquid-liquid extraction procedure for tissues samples (lung, liver and kidney). The between-day and within-day coefficients of variation for all compounds were </= 20% at the concentrations of lower limit of quantification (LLOQ) and </= 15% at other quality control concentrations. The same method was also partially validated for human plasma. The utility of this assay was demonstrated by monitoring BDP and 17-BMP plasma and tissue concentrations in an animal study designed for evaluation of pulmonary targeting after intratracheal administration of BDP dry powder.
Wang
Yaning
Y
Department of Pharmaceutics, College of Pharmacy, University of Florida, Gainesville, FL 32610, USA.
Hochhaus
Günther
G
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000097:blood*; D001507:Beclomethasone / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D051381:Rats; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000893:Anti-Inflammatory Agents; D001507:Beclomethasone