title: Expression of clusterin in Müller cells of the rat retina after pressure-induced ischemia.
abstract: We have investigated the expression and cellular localization of clusterin in the rat retina following ischemia induced by transiently increasing the intraocular pressure. In the normal retina, weak clusterin immunoreactivity was visible in Müller cell profiles located in the inner nuclear layer. Following ischemia and reperfusion, strong immunoreactivity appeared in Müller cell somata and processes up to 3 days postlesion. Quantitative evaluation by immunoblotting confirmed that clusterin expression continuously increased and showed a peak value at 3 days after ischemic injury (to 1300% of control levels), and then decreased again to 400% of controls at 4 weeks postlesion. Immunocytochemistry using antisera against clusterin or glutamine synthase combined with the TUNEL method or immunocytochemistry using antisera activated caspase 3 and electron microscopy revealed that some clusterin-labeled Müller cells underwent apoptotic cell death. Our findings demonstrate that some Müller cells die by apoptosis, and suggest that clusterin produced and released by Müller cell may play an important role in the pathogenesis of ischemic injury in the rat retina.
authors.affiliation: Department of Anatomy, College of Medicine, Catholic University of Korea, Seoul, Korea.
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Gwon
authors.forename: In-Beom
authors.initials: IB
authors.lastname: Kim
authors.forename: Mun-Yong
authors.initials: MY
authors.lastname: Lee
authors.forename: Su-Ja
authors.initials: SJ
authors.lastname: Oh
authors.forename: Myung-Hoon
authors.initials: MH
authors.lastname: Chun
mesh_terms: D000818:Animals; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D051152:Clusterin; D004195:Disease Models, Animal; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D007150:Immunohistochemistry; D007429:Intraocular Pressure; D007511:Ischemia / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D008854:Microscopy, Electron; D018832:Molecular Chaperones / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D012160:Retina / Q000378:metabolism* / Q000503:physiopathology / Q000648:ultrastructure; D012164:Retinal Diseases / Q000378:metabolism* / Q000503:physiopathology; D015854:Up-Regulation
chemical_list: C498500:Clu protein, rat; D051152:Clusterin; D006023:Glycoproteins; D018832:Molecular Chaperones; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases; D005974:Glutamate-Ammonia Ligase