Characterization of 298 ESTs from porcine back fat tissue and their assignment to the SSRH radiation hybrid map.
Following several criteria, we collected, clustered, and functionally categorized 653 expressed sequence tags (ESTs) of 5' ends from porcine back fat libraries from the >15,000 porcine ESTs collected to date. By searching the LocusLink and Mapviewer database, we knew the positions of these 653 ESTs on human chromosomes (HSAs). Sus scrofa radiation hybrid (SSRH) mapping revealed that 298 porcine EST clusters out of 653 were localized near microsatellite (MS) markers. Among these EST clusters, we could assign 182 to their porcine chromosomes (SSCs) on the SSRH map.
Mikawa
Ayumi
A
Animal Genome Research Program Team, Society for Techno-Innovation of Agriculture, Forestry and Fisheries Institute, Tsukuba, Ibaraki 305-0854, Japan.
Suzuki
Hideaki
H
Suzuki
Kohei
K
Toki
Daisuke
D
Uenishi
Hirohide
H
Awata
Takashi
T
Hamasima
Noriyuki
N
D000273:Adipose Tissue / Q000502:physiology*; D000818:Animals; D016000:Cluster Analysis; D004247:DNA / Q000737:chemistry / Q000235:genetics; D020224:Expressed Sequence Tags; D015723:Gene Library; D006801:Humans; D051379:Mice; D018895:Microsatellite Repeats / Q000235:genetics; D021101:Radiation Hybrid Mapping / Q000662:veterinary; D013552:Swine / Q000235:genetics*
D004247:DNA