[Search for transcribed segments in the region of q14.3 of human chromosome 13 in silico].
Using computer-aided genomic methods, a complete map of the expressed sequence tags (EST) located in the human genome region 13q14.3 between the STS markers, D13S810 and D13S1469, was constructed. A total of 62 EST clusters were formed, of which 12 clusters corresponded to the already known human genes, 4 clusters represented pseudogenes, and 10 clusters were new human genes. The use of the method of reverse transcription in combination with polymerase chain reaction (RT-PCR) provided experimental confirmation of the existence of mRNA transcripts for the novel human genes revealed in silico.
Tiazhelova
T V
TV
Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991 Russia. tvt@vigg.ru
Ivanov
D V
DV
Nazarenko
S A
SA
Baranova
A V
AV
Iankovskiĭ
N K
NK
D001483:Base Sequence; D002882:Chromosomes, Human, Pair 13; D017931:DNA Primers; D020224:Expressed Sequence Tags; D006801:Humans; D005810:Multigene Family; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D012333:RNA, Messenger