title: Micropatterned surfaces prepared using a liquid crystal projector-modified photopolymerization device and microfluidics.
abstract: A commercial liquid crystal device projector was modified for photopolymerization using its on-board intense light source and a precision optical control circuit. This device projects reduced images generated by a typical personal computer onto the stage where photopolymerization on a surface occurs. This all-in-one device does not require expensive photomasks and external light sources. However, light scattering and diffraction through glass substrates resulted in undesired reactions in areas corresponding to masked (black) domains in mask patterns, limiting pattern resolution. To overcome this shortcoming, two-step surface patterning was developed. First, three-dimensional microstructures of crosslinked silicone elastomer were fabricated with this device and adhered onto silanized glass substrate surfaces, forming microchannels in patterns on the glass support. Then, acrylamide monomer solution containing photoreactive initiator was flowed into these micromold channels and reacted in situ. The resultant polyacrylamide layer was highly hydrophilic and repelled protein adsorption. Cell seeding on these patterns in serum-supplemented culture medium produced cells selectively adhered to different patterns: cells attached and spread only on unpolymerized silanized glass surfaces, not on the photopolymerized acrylamide surfaces. This technique should prove useful for inexpensive, rapid prototyping of surface micropatterns from polymer materials.
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, CREST-JST, Kawada-cho 8-1, Shinjuku-ku, Tokyo 162-8666, Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Itoga
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.forename: Jun
authors.initials: J
authors.lastname: Kobayashi
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kikuchi
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D004129:Dimethylpolysiloxanes / Q000737:chemistry / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology; D008422:Materials Testing; D044085:Microfluidics; D010777:Photochemistry; D011108:Polymers / Q000737:chemistry; D012828:Silicones / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D004129:Dimethylpolysiloxanes; D011108:Polymers; D012828:Silicones; D000099166:Polyacrylamides; C013830:baysilon