title: Structure of a highly phosphorylated O-polysaccharide of Proteus mirabilis O41.
abstract: A highly phosphorylated O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Proteus mirabilis O41 followed by GPC. The initial and dephosphorylated polysaccharides and phosphorylated products from two sequential Smith degradations were studied by (1)H, (13)C and (31)P NMR spectroscopy and ESI-MS. The O-polysaccharide was found to have a tetrasaccharide repeating unit containing one ribitol phosphate (presumably d-Rib-ol-5-P) and two ethanolamine phosphate (Etn-P) groups, one of which is present in the stoichiometric amount and the other in a nonstoichiometric amount. The following structure of the O-polysaccharide was established:
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Sof'ya N
authors.initials: SN
authors.lastname: Senchenkova
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Perepelov
authors.forename: Maciej
authors.initials: M
authors.lastname: Cedzynski
authors.forename: Anna S
authors.initials: AS
authors.lastname: Swierzko
authors.forename: Andrzej
authors.initials: A
authors.lastname: Ziolkowski
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Wieslaw
authors.initials: W
authors.lastname: Kaca
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
authors.forename: Per-Erik
authors.initials: PE
authors.lastname: Jansson
mesh_terms: D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D010428:Pentosephosphates / Q000737:chemistry; D010766:Phosphorylation; D011513:Proteus mirabilis / Q000737:chemistry*
chemical_list: D019081:O Antigens; D010428:Pentosephosphates; C041025:ribitol-5-phosphate