title: Fluorescence in situ hybridization analysis of nitrifiers in piggery wastewater treatment reactors.
abstract: Fluorescence in situ hybridization (FISH) was performed to analyze the nitrifying microbial communities in an activated sludge reactor (ASR) and a fixed biofilm reactor (FBR) for piggery wastewater treatment. Heterotrophic oxidation and nitrification were occurring simultaneously in the ASR and the COD and nitrification efficiencies depend on the loads. In the FBR nitrification efficiency also depends on ammonium load to the reactor and nitrite was accumulated when free ammonia concentration was higher than 0.2 mg NH3-N/L. FISH analysis showed that ammonia-oxidizing bacteria (NSO1225) and denitrifying bacteria (RRP1088) were less abundant than other bacteria (EUB338) in ASR. Further analysis on nitrifying bacteria in the FBR showed that Nitrosomonas species (NSM156) and Nitrospira species (NSR1156) were the dominant ammonia-oxidizing and nitrite-oxidizing bacteria, respectively, in the piggery wastewater nitrification system.
authors.affiliation: Department of Environmental Systems Engineering, Hallym University, Chunchon, Kangwon 200-70, Korea. dongjin@hallym.ac.kr
authors.forename: D J
authors.initials: DJ
authors.lastname: Kim
authors.forename: T K
authors.initials: TK
authors.lastname: Kim
authors.forename: E J
authors.initials: EJ
authors.lastname: Choi
authors.forename: W C
authors.initials: WC
authors.lastname: Park
authors.forename: T H
authors.initials: TH
authors.lastname: Kim
authors.forename: D H
authors.initials: DH
authors.lastname: Ahn
authors.forename: Z
authors.initials: Z
authors.lastname: Yuan
authors.forename: L
authors.initials: L
authors.lastname: Blackall
authors.forename: J
authors.initials: J
authors.lastname: Keller
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000254:growth & development; D019149:Bioreactors; D004269:DNA, Bacterial / Q000032:analysis*; D017404:In Situ Hybridization, Fluorescence; D008372:Manure; D009584:Nitrogen / Q000302:isolation & purification* / Q000378:metabolism*; D009606:Nitrosomonas / Q000235:genetics / Q000254:growth & development; D011157:Population Dynamics; D012722:Sewage / Q000382:microbiology*; D013552:Swine; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D004269:DNA, Bacterial; D008372:Manure; D012722:Sewage; D009584:Nitrogen