Oxy-peptide nucleic acid with a pyrrolidine ring that is configurationally optimized for hybridization with DNA.
Four stereoisomers of oxy-peptide nucleic acids containing ether linkages in the main chain and conformationally-restricted pyrrolidine rings (pyrrolidine-based oxy-PNA = POPNA) were newly synthesized and investigated for binding to DNA. cis-l-POPNA with 9 adenine bases formed the most stable hybrid with dT(9). The POPNA showed high sequence specificity similar to that of the Nielsen-type PNA and sharper melting behavior in hybridization with DNA than the Nielsen-type PNA.
Kitamatsu
Mizuki
M
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.
Shigeyasu
Masanori
M
Okada
Tomoyuki
T
Sisido
Masahiko
M
D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D006824:Hybridization, Genetic / Q000502:physiology*; D008968:Molecular Conformation; D009696:Nucleic Acids / Q000737:chemistry / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D011759:Pyrrolidines / Q000737:chemistry / Q000378:metabolism*
D009696:Nucleic Acids; D010446:Peptide Fragments; D011759:Pyrrolidines; D004247:DNA