title: Isolation and partial characterization of fucan sulfates from the body wall of sea cucumber Stichopus japonicus and their ability to inhibit osteoclastogenesis.
abstract: Two types of fucan sulfate were isolated from chloroform/methanol extract of the body wall of the sea cucumber Stichopus japonicus. One type (type A) contained 3.41 mmol fucose/g and 2.35 mmol sulfate/g, and the molecular mass was determined to be 9 kDa by gel permeation chromatography (GPC). Structural analysis suggested that type A consists of a backbone of (1-->3)-linked fucosyl residues that are substituted at C-4 with fucosyl residues, and that fucosyl residues are sulfated at C-2 and/or C-4. Another type (type B) contained 3.90 mmol fucose/g and 3.07 mmol sulfate/g, and the molecular mass was determined to be 32kDa by GPC. Structural analysis showed that type B is largely composed of unbranched (1-->3)-linked fucosyl residues, and that sulfate substitution(s) occur at C-2 and/or C-4. The potential of both types to inhibit osteoclastogenesis was examined by an in vitro assay system, showing that both types of fucan sulfate inhibit osteoclastogenesis more than 95% at 50 microg/mL concentration. These results suggest that types A and B fucan sulfate from sea cucumber are potent inhibitors of osteoclastogenesis.
authors.affiliation: Central Research Laboratories, Seikagaku Corporation, 3-1253 Tateno, Higashiyamato, Tokyo 207-0021, Japan. kariya@seikagaku.co.jp
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kariya
authors.forename: Barbara
authors.initials: B
authors.lastname: Mulloy
authors.forename: Kyoko
authors.initials: K
authors.lastname: Imai
authors.forename: Akihiro
authors.initials: A
authors.lastname: Tominaga
authors.forename: Takuji
authors.initials: T
authors.lastname: Kaneko
authors.forename: Akira
authors.initials: A
authors.lastname: Asari
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Suzuki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Masuda
authors.forename: Mamoru
authors.initials: M
authors.lastname: Kyogashima
authors.forename: Tadashi
authors.initials: T
authors.lastname: Ishii
mesh_terms: D000818:Animals; D001681:Biological Assay; D001862:Bone Resorption / Q000188:drug therapy; D015394:Molecular Structure; D010010:Osteoclasts / Q000187:drug effects*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D047528:Stichopus / Q000737:chemistry*
chemical_list: D011134:Polysaccharides; C007789:fucoidan