Persistent increase of matrix metalloproteinases in cerebrospinal fluid of tuberculous meningitis.
Matrix metalloproteinase (MMP)-2 and MMP-9 were analyzed by gelatin zymography and an enzyme-linked immunosorbent assay (ELISA) in a cerebrospinal fluid (CSF) from patients with tuberculous meningitis (n=24), acute aseptic meningitis (n=23) and the control (n=10). The MMP-2 and MMP-9 levels were significantly higher in the samples from the tuberculous meningitis patients than those from either the aseptic meningitis patients or the controls. In tuberculous meningitis, the patients with late neurologic complications had higher MMP-2 and MMP-9 levels than those without. The persistent increase in the MMP-2 and MMP-9 levels was associated with the development of complications following tuberculous meningitis. Inhibiting the MMPs may be an effective strategy for preventing or reducing the complications in tuberculous meningitis.
Lee
Kyung Yul
KY
Department of Neurology, Yongdong Severance Hospital, Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul, South Korea.
Kim
Eun Hee
EH
Yang
Won Seok
WS
Ryu
Hyunjeong
H
Cho
Sang-Nae
SN
Lee
Byung In
BI
Heo
Ji Hoe
JH
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000134:cerebrospinal fluid*; D020780:Matrix Metalloproteinase 9 / Q000134:cerebrospinal fluid*; D008582:Meningitis, Aseptic / Q000134:cerebrospinal fluid / Q000201:enzymology; D014390:Tuberculosis, Meningeal / Q000134:cerebrospinal fluid* / Q000201:enzymology
D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9