title: Biomarker discovery by comprehensive phenotyping for autoimmune diseases.
abstract: There is a well-recognized but unmet need for biological markers to characterize disease type, status, progression, and response to therapy in autoimmune diseases. We are developing and applying an integrated bioanalytical platform and clinical research program to facilitate comprehensive differential phenotyping of patient samples and enable the discovery of biomarkers. Our measurement platform includes microvolume laser scanning cytometry for the quantification of hundreds of cellular parameters in whole blood and other samples, liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry for the quantification of proteins and low molecular weight biomolecules in serum and other fluids or tissues, and specific immunoassays for the quantification of trace proteins in serum. We describe the technologies and discuss initial applications to the analysis of subjects with rheumatoid arthritis (RA) and healthy controls.
authors.affiliation: SurroMed, Inc., Menlo Park, CA 94025, USA. akantor@surromed.com
authors.forename: Aaron B
authors.initials: AB
authors.lastname: Kantor
authors.forename: Weixun
authors.initials: W
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Lin
authors.forename: Harini
authors.initials: H
authors.lastname: Govindarajan
authors.forename: Markus
authors.initials: M
authors.lastname: Anderle
authors.forename: Andrea
authors.initials: A
authors.lastname: Perrone
authors.forename: Christopher
authors.initials: C
authors.lastname: Becker
mesh_terms: D001172:Arthritis, Rheumatoid / Q000097:blood* / Q000276:immunology*; D015415:Biomarkers / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D019044:Image Cytometry / Q000379:methods; D017846:Longitudinal Ligaments; D008297:Male; D010641:Phenotype; D040901:Proteomics / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D015415:Biomarkers