Local acute application of BDNF in the lesioned spinal cord anti-inflammatory and anti-oxidant effects.
We studied the early anti-inflammatory and anti-oxidant effects of local application of BDNF after dorsal spinal cord transection in the adult rat. Both the distribution and accumulation of neutrophils and microglial cells in and around the lesion site (inflammatory response) and the accumulation of lipid peroxidation product 4-hydroxynonenal (HNE; oxidative damage) around the lesion was examined using immunohistochemical techniques. We demonstrate that BDNF application affects the microglial response in and around the lesion and results in a reduced lipid peroxidation as shown by HNE-immunoreactive staining around the lesion 48 h post-injury. The early anti-inflammatory and anti-oxidant effects of local BDNF-application into the lesioned spinal cord may contribute to the observed decreased loss of locomotor function of the hindlimbs 2 days after injury.
Joosten
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EA
Division of Cellular Neuroscience, Room 1.112, European Graduate School for Neuroscience, Faculty of Medicine, UNS 50, University of Maastricht, 6200 MD Maastricht, The Netherlands. b.joosten@np.unimaas.nl
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D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage*; D000975:Antioxidants / Q000008:administration & dosage*; D019208:Brain-Derived Neurotrophic Factor / Q000008:administration & dosage*; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013904:Thoracic Vertebrae / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D019208:Brain-Derived Neurotrophic Factor