title: Dual role of polymorphonuclear neutrophils on the growth of Ehrlich ascites tumor (EAT) in mice.
abstract: We show that granulocytes (PMN) have a dual role in the development of Ehrlich Ascites Tumor (EAT) in mice. EAT intraperitoneal inoculation causes a local inflammatory reaction, ascites development and mortality that distinguish resistant and susceptible strains. In resistant mice (CAF1), there is a less pronounced PMN influx after EAT inoculation than in susceptible Swiss mice. Accordingly, the increase in peritoneal PMN numbers enhanced tumor growth in CAF1 mice, but had no effect in the susceptible Swiss animals. Contrastingly, PMN depletion had no effect in resistant mice but facilitated tumor growth in susceptible animals. Though no differences were noted between the strains in peritoneal cell spreading and hydrogen peroxide release after tumor inoculation, in vitro PMN cytotoxic activity against EAT was significantly higher in susceptible Swiss mice. These data indicate a paradoxical dual role for PMN against EAT: while they help control tumor development in susceptible animals, they seem to enhance tumor growth in resistant mice.
authors.affiliation: Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Professor Lineu Prestes, 1730, Cidade Universitária, CEP 05508-000, São Paulo, Brazil.
authors.forename: Patrícia C
authors.initials: PC
authors.lastname: Bergami-Santos
authors.forename: Mário
authors.initials: M
authors.lastname: Mariano
authors.forename: José Alexandre M
authors.initials: JA
authors.lastname: Barbuto
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001835:Body Weight; D002286:Carcinoma, Ehrlich Tumor / Q000150:complications / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D006003:Glycogen / Q000276:immunology / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007249:Inflammation / Q000209:etiology / Q000276:immunology*; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009504:Neutrophils / Q000276:immunology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D013778:Tetrazolium Salts; D013844:Thiazoles; D006003:Glycogen; D006861:Hydrogen Peroxide; C022616:thiazolyl blue