title: Effect of sodium arsenite on peripheral lymphocytes in vitro: individual susceptibility among a population exposed to arsenic through the drinking water.
abstract: Arsenic (As) contamination in ground water has affected more than 19 countries. Approximately 36 million people in the Bengal delta alone are exposed to this toxicant via drinking water (>50 microg/l) and are at potential health risk. Chronic ingestion of As via drinking water is associated with occurrence of skin lesions, cancer and other arsenic-induced diseases in West Bengal, India. An in vitro cytogenetic study was performed utilizing chromosomal aberrations (CA) in lymphocytes treated with sodium arsenite (0-5 microM) in six symptomatic (having arsenic-related skin lesions) individuals, six age- and sex-matched As-exposed asymptomatic (no arsenic-related skin lesions) individuals and six control individuals with similar socio-economic status residing in non-affected districts of West Bengal with no evidence of As exposure. The mean As content in nails and hair was 9.61 and 5.23 microg/g in symptomatic, 3.48 and 2.17 microg/g in asymptomatic and 0.42 and 0.33 microg/g in the control individuals, respectively. The main aim of our study was to determine whether genotoxic effects differed in the lymphocytes of the control (no exposure to arsenic), asymptomatic and symptomatic individuals after in vitro treatment with sodium arsenite. Although both the exposed groups had chronic exposure to As through the drinking water, individuals with skin lesions accumulated more As in their nails and hair and excreted less in urine (127.80 versus 164.15 microg/l). The results show that sodium arsenite induced a significantly higher percentage of aberrant cells in the lymphocytes of control individuals than in the lymphocytes of both the exposed groups. Within the two exposed groups As induced higher incidences of CA in the symptomatic than the asymptomatic individuals. These results suggest that asymptomatic individuals have relatively lower sensitivity and susceptibility to induction of genetic damage by As compared with the symptomatic individuals.
authors.affiliation: Division of Human Genetics and Genomics, Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, Jadavpur, Kolkata-700032, India.
authors.forename: Julie
authors.initials: J
authors.lastname: Mahata
authors.forename: Pritha
authors.initials: P
authors.lastname: Ghosh
authors.forename: Jyotirindra N
authors.initials: JN
authors.lastname: Sarkar
authors.forename: Kunal
authors.initials: K
authors.lastname: Ray
authors.forename: Adayapalam T
authors.initials: AT
authors.lastname: Natarajan
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Giri
mesh_terms: D000328:Adult; D001151:Arsenic / Q000494:pharmacology / Q000652:urine; D018053:Arsenites / Q000494:pharmacology*; D002869:Chromosome Aberrations / Q000139:chemically induced; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006197:Hair / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects*; D009262:Nails / Q000378:metabolism; D017670:Sodium Compounds / Q000494:pharmacology*; D014874:Water Pollutants, Chemical / Q000494:pharmacology
chemical_list: D018053:Arsenites; D004791:Enzyme Inhibitors; D017670:Sodium Compounds; D014874:Water Pollutants, Chemical; C017947:sodium arsenite; D001151:Arsenic