The influence of filling technique on depth of tubule penetration by root canal sealer: a study using light microscopy and digital image processing.
The purpose of this study was to compare the depth of sealer penetration into dentinal tubules by three root-filling techniques using light microscopy and digital image processing. Thirty-two maxillary central incisors were prepared. Two teeth were separated for the control group. The rest were divided into three equal groups and obturated as following--G1: lateral condensation; G2: warm vertical compaction of gutta-percha and G3: Thermafil system. Each sample was sectioned longitudinally and prepared for microscopic analysis. A sequence of photomicrographs with magnifications of X50, X200 and X500 were taken. Through digital image analysis and processing, measurements for each field were obtained. A non-parametric ANOVA Kruskal-Wallis analysis was used to determine whether there were significant differences among the groups. Significant differences between G2 and G1 (p = 0.034) and between G3 and G1 (p = 0.021) were identified. There were no significant differences between G2 and G3 (p > 0.05). The results of this research suggest that samples root-filled by thermoplasticised gutta-percha techniques lead to deeper penetration of the root canal sealer into the dentinal tubules.
De Deus
Gustavo A
GA
Department of Endodontics, Rio de Janeiro State University--UERJ, Brazil.
Gurgel-Filho
Eduardo Diogo
ED
Maniglia-Ferreira
Cláudio
C
Coutinho-Filho
Tauby
T
D000704:Analysis of Variance; D003804:Dentin / Q000648:ultrastructure*; D006171:Gutta-Percha / Q000737:chemistry; D006358:Hot Temperature; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007180:Incisor; D008853:Microscopy; D010784:Photomicrography; D012387:Root Canal Filling Materials / Q000737:chemistry*; D012389:Root Canal Obturation / Q000379:methods*; D018915:Root Canal Preparation; D018709:Statistics, Nonparametric
D012387:Root Canal Filling Materials; D006171:Gutta-Percha