title: Detection of multidrug resistant gene 1 in pancreatic cancer.
abstract: BACKGROUND
  The results are conflicting in detecting P-glycoprotein (P-gp) in pancreatic cancer. The aim of this study was to detect the expression of multidrug resistant gene 1 (MDR1) in pancreatic cancer cell lines.
  
  
  METHODS
  MDR1 mRNA and P-gp were detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical assay (IHC) in three pancreatic cancer cell lines SW1990, CAPAN-1 and P3. P-gp functions were evaluated through the rhodamine extrusion test.
  
  
  RESULTS
  Two of the three cell lines expressed MDR1 positively at different levels. The rhodamine extrusion test showed that the percentage of positive cells in MDR(+) cells was significantly lower than that in MDR1(-) cells. The results of IHC, RT-PCR and the rhodamine extrusion test were consistent with each other.
  
  
  CONCLUSIONS
  All of these methods are reliable in the detection of MDR1 in pancreatic cancer tissue, thus providing a guide for clinical chemotherapy of pancreatic cancer.
authors.affiliation: Department of General Surgery, Peking Union Medical College Hospital, Beijing 100730, China. zhao8028@263.net
authors.forename: Yu-Pei
authors.initials: YP
authors.lastname: Zhao
authors.forename: Li-Yang
authors.initials: LY
authors.lastname: Zhang
authors.forename: Quan
authors.initials: Q
authors.lastname: Liao
authors.forename: Jun-Chao
authors.initials: JC
authors.lastname: Guo
authors.forename: Ge
authors.initials: G
authors.lastname: Chen
authors.forename: Jia-Yi
authors.initials: JY
authors.lastname: Li
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D015870:Gene Expression / Q000235:genetics; D019450:Genes, MDR / Q000235:genetics*; D006801:Humans; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D012333:RNA, Messenger