title: Glycerophosphodiester phosphodiesterase gene (glpQ) of Borrelia lonestari identified as a target for differentiating Borrelia species associated with hard ticks (Acari:Ixodidae).
abstract: A glpQ ortholog was identified in DNA from Borrelia lonestari-positive Amblyomma americanum, providing further evidence that B. lonestari is more closely related to the relapsing fever group spirochetes than to borreliae that cause Lyme disease. This finding provides a basis for developing diagnostic assays to differentiate species of borrelia transmitted by hard ticks.
authors.affiliation: Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80521, USA. rbacon@cdc.gov
authors.forename: Rendi Murphree
authors.initials: RM
authors.lastname: Bacon
authors.forename: Mark A
authors.initials: MA
authors.lastname: Pilgard
authors.forename: Barbara J B
authors.initials: BJ
authors.lastname: Johnson
authors.forename: Sandra J
authors.initials: SJ
authors.lastname: Raffel
authors.forename: Tom G
authors.initials: TG
authors.lastname: Schwan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D001898:Borrelia / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D001899:Borrelia Infections / Q000175:diagnosis / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D026863:Ixodidae / Q000382:microbiology*; D008969:Molecular Sequence Data; D010727:Phosphoric Diester Hydrolases / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D010727:Phosphoric Diester Hydrolases; C087921:glpQ protein, Bacteria