Immune tolerance in pancreatic islet xenotransplantation.
OBJECTIVE
To observe the effect of tail vein injection with donor hepatocytes and/or splenocytes on the islet xenotransplantation rejection.


METHODS
New-born male pigs and BALB/C mice were selected as donors and recipients respectively. Islet xenotransplantation was performed in recipients just after the third time of tail vein injection with donor hepatocytes and/or splenocytes. Macrophage phagocytosis, NK(natural killing cell) killing activity, T lymphocyte transforming function of spleen cells, antibody forming function of B lymphocytes, and T lymphocyte subsets were taken to monitor transplantation rejection. The effects of this kind of transplantation were indicated as variation of blood glucose and survival days of recipients.


RESULTS
The results showed that streptozotocin (STZ) could induce diabetes mellitus models of mice. The pre-injection of donor hepatocytes, splenocytes or their mixture by tail vein injection was effective in preventing donor islet transplantation from rejection, which was demonstrated by the above-mentioned immunological marks. Each group of transplantation could decrease blood glucose in recipients and increase survival days. Pre-injection of mixture of donor hepatocytes and splenocytes was more effective in preventing rejection as compared with that of donor hepatocyte or splenocyte pre-injection respectively.


CONCLUSIONS
Pre-injection of donor hepatocytes, splenocytes or their mixture before donor islet transplantation is a good way in preventing rejection.
Tang
Tian-Hua
TH
Department of Hemato-oncology, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jingshi Road 89, Jinan 250062, Shandong Province, China.
Li
Chun-Lin
CL
Li
Xin
X
Jiang
Feng-Qin
FQ
Zhang
Yu-Kun
YK
Ren
Hai-Quan
HQ
Su
Shan-Shan
SS
Jiang
Guo-Sheng
GS
D000818:Animals; D000831:Animals, Newborn; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000276:immunology; D005260:Female; D006084:Graft Rejection / Q000517:prevention & control*; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D007108:Immune Tolerance / Q000276:immunology / Q000502:physiology*; D016381:Islets of Langerhans Transplantation; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology; D013552:Swine; D014183:Transplantation, Heterologous
D001786:Blood Glucose