Genomics in the immune system.
The analysis of gene expression in tissues, cells, and biologic systems has evolved in the last decade from the analysis of a selected set of genes to an efficient high throughput whole-genome screening approach of potentially all genes expressed in a tissue or cell sample. Development of sophisticated methodologies such as microarray technology allows an open-ended survey to identify comprehensively the fraction of genes that are differentially expressed between samples and define the samples' unique biology. This discovery-based research provides the opportunity to characterize either new genes with unknown function or genes not previously known to be involved in a biologic process. The latter category may hold surprises that sometimes urge us to redirect our thinking. Here, we review the impact of large-scale gene expression profiling by DNA-microarray technology on basic and clinical aspects of immunology.
van der Pouw Kraan
Tineke C M T
TC
Department of Molecular Cell Biology and Immunology, Amsterdam, VU Medical Center, The Netherlands.
Kasperkovitz
Pia V
PV
Verbeet
Nicolette
N
Verweij
Cornelis L
CL
D001327:Autoimmune Diseases / Q000235:genetics / Q000276:immunology; D003141:Communicable Diseases / Q000235:genetics / Q000276:immunology; D020869:Gene Expression Profiling / Q000379:methods; D023281:Genomics / Q000379:methods*; D006801:Humans; D007107:Immune System / Q000502:physiology*; D007111:Immunity, Cellular / Q000235:genetics / Q000276:immunology; D007113:Immunity, Innate / Q000235:genetics / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods