Effect of freeze-thawing and polyethylene glycol (PEG) lipid on fusion and fission of phospholipid vesicles.
The effect of freeze-thawing on the size of egg yolk phosphatidylcholine (EggPC) vesicles in the presence of 0-40 mol% distearoylphosphatidylethanolamine-polyethylene glycol 2000 (DSPE-PEG) was studied. Mean diameters of the vesicles fell into a range of 80-150 nm after 10 times freeze-thawing in spite of their original size. In the process of freeze-thawing, two opponent events, one is fission and the other is fusion, occurred at the same time. DSPE-PEG accelerated the fusion event.
Sriwongsitanont
Supaporn
S
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Ueno
Masaharu
M
D005615:Freezing; D008081:Liposomes; D010743:Phospholipids / Q000032:analysis / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000032:analysis / Q000737:chemistry*
D008081:Liposomes; D010743:Phospholipids; D011092:Polyethylene Glycols