title: Simple staining detects ultrastructural and biochemical differentiation of vegetative hyphae and fruit body initials in colonies of Pleurotus pulmonarius.
abstract: OBJECTIVE
  To know the ultrastructural and biochemical differences of vegetative hyphae and fruit body initials in colonies of Pleurotus pulmonarius.
  
  
  RESULTS
  Feulgen reagent was used to detects differentiation of hyphae. The intracellular laccases, proteases and beta-1,3-glucanases activity, content of cytoplasmic protein, glycogen and glucans in the cell wall were evaluated in hyphae of fruit body initials and in vegetative hyphae. The thickness of hyphal walls of the vegetative hyphae was also evaluated. Substantial biochemical changes were observed in hyphae of different zones of the fruiting colony. Hyphae at the periphery had thinner walls than in the centre of the colony.
  
  
  CONCLUSIONS
  Staining correlated with the enzymatic activity, protein, glycogen and glucans, in mycelium and in fruit body initials. The implications are that hyphal maturity in P. pulmonarius involves storage of glucans, in part at least, in the form of a thickened hyphal wall.
authors.affiliation: Laboratory of Biotechnology, Research Centre for Biological Sciences, Universidad Autónoma de Tlaxcala, Tlaxcala, México. sanher@cci.uatx.mx
authors.forename: C
authors.initials: C
authors.lastname: Sánchez
authors.forename: M
authors.initials: M
authors.lastname: Téllez-Téllez
authors.forename: G
authors.initials: G
authors.lastname: Díaz-Godínez
authors.forename: D
authors.initials: D
authors.lastname: Moore
mesh_terms: D002473:Cell Wall / Q000737:chemistry / Q000648:ultrastructure; D003593:Cytoplasm / Q000737:chemistry / Q000201:enzymology; D010450:Endopeptidases / Q000032:analysis / Q000378:metabolism; D005656:Fungal Proteins / Q000032:analysis; D043326:Glucan 1,3-beta-Glucosidase / Q000032:analysis / Q000378:metabolism; D005936:Glucans / Q000032:analysis; D006003:Glycogen / Q000032:analysis; D025301:Hyphae / Q000737:chemistry* / Q000201:enzymology* / Q000648:ultrastructure; D042845:Laccase / Q000032:analysis / Q000378:metabolism; D008854:Microscopy, Electron; D020076:Pleurotus / Q000737:chemistry* / Q000201:enzymology* / Q000254:growth & development / Q000648:ultrastructure; D012394:Rosaniline Dyes / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*
chemical_list: C007722:Feulgen stain; D005656:Fungal Proteins; D005936:Glucans; D012394:Rosaniline Dyes; D006003:Glycogen; D042845:Laccase; D043326:Glucan 1,3-beta-Glucosidase; D010450:Endopeptidases