Collaborative study of the NCCLS and flow cytometry methods for antifungal susceptibility testing of Candida albicans.
One hundred clinical isolates of Candida albicans were tested for amphotericin B and fluconazole susceptibilities by the National Committee for Clinical Laboratory Standards (NCCLS) broth microdilution test at center 1 (C1). The same isolates were tested blinded at center 2 (C2) by NCCLS and flow cytometry (FC) methods. The agreement between NCCLS and FC methods ranged from 96 to 99%.
Chaturvedi
Vishnu
V
Mycology Laboratory, Wadsworth Center, New York State Department of Health, Albany, 12208-2002, USA. vishnu@wadsworth.org
Ramani
Rama
R
Pfaller
Michael A
MA
D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000302:isolation & purification; D025141:Drug Resistance, Fungal; D005434:Flow Cytometry / Q000379:methods; D015725:Fluconazole / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000706:statistics & numerical data
D000935:Antifungal Agents; D000666:Amphotericin B; D015725:Fluconazole