Could prolactin receptor gene polymorphism play a role in pathogenesis of breast carcinoma?
OBJECTIVE
Constitutive activation of various hormone and growth factor receptors is newly recognised as a common cause of tumour development. This study investigated the presence of any mutation or polymorphism of prolactin receptor (PRLR) in 38 patients with breast cancer.


METHODS
Genomic DNA was extracted and PCR amplification was carried out for exon 1-10 of PRLR from tumoral and adjacent non-cancerous breast tissue of tumour specimens from 38 breast cancer patients. PCR products were analysed by SSCP and automatic sequencing for mutations.


RESULTS
For the first time, A150C (Leu-->Ile) transversion at exon 6 of PRLR in tumour tissues, in adjacent non-cancerous breast tissues, and in blood samples of two (5.3%) out of 38 patients with breast cancer were detected. In contrast to this finding, no polymorphism of PRLR in blood samples of 100 normal individuals were found.


CONCLUSIONS
Polymorphism of prolactin receptors might play a role in mammary carcinogenesis as a consequence of intracellular changes of PRLR signalling.
Canbay
Emel
E
Department of General Surgery, Cumhuriyet University Hospital, Sivas, Turkey. canbay@cumhuriyet.edu.tr
Degerli
Naci
N
Gulluoglu
Bahadir M
BM
Kaya
Handan
H
Sen
Metin
M
Bardakci
Fevzi
F
D000328:Adult; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D005091:Exons; D005260:Female; D006801:Humans; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D011960:Receptors, Estrogen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis; D011981:Receptors, Prolactin / Q000235:genetics*; D017422:Sequence Analysis, DNA
D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D011981:Receptors, Prolactin