Effects of periodontal dressings on fibroblasts and gingival wound healing in dogs.
In the present study the effects of different commercially available periodontal dressings (Peripac, Barricaid, Fittydent, Reso-Pack and Myzotect-tincture) on fibroblast (V-79-379A) proliferation and survival were tested in vitro. Barricaid, Fittydent and Reso-Pack periodontal dressings have only small inhibitory effects on cell proliferation (83.3 +/- 9%, 71.6 +/- 8.7% and 87.3 +/- 4.5% of control after 48 h, respectively) in comparison with the great inhibitory effect of Myzotect-tincture (2.9 +/- 0.1%) and Peripac (33.7 +/- 11.4%) (p < 0.001). Barricaid was the only dressing where 41% of cells survived after exposure, while the other four dressings killed all the cells in 6 days. In addition, the healing of artificially created gingival wounds covered by Barricaid and Reso-Pack was followed for 7 days in 12 Beagle dogs. Histological evaluation of gingival tissue demonstrated that wounds covered by Reso-Pack showed the best epithelisation and vascularity and the least inflammatory reaction in first 4 days. Later the observed parameters were similar with those of wounds covered by Barricaid or without pack. The present results indicate that Peripac periodontal dressing and Myzotect-tincture showed the highest cytotoxicity to fibroblasts in vitro. From the histological observations in Beagle dogs Reso-Pack has been found to be the most suitable dressing, followed by Barricaid.
Petelin
M
M
Department of Oral Medicine and Periodontology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia. milan.petelin@mf.uni-lj.si
Pavlica
Z
Z
Batista
Urska
U
Stiblar-Martincic
Draga
D
Skaleric
U
U
D000269:Adhesives / Q000494:pharmacology*; D000818:Animals; D006224:Cricetinae; D003764:Dental Materials / Q000494:pharmacology*; D004285:Dogs / Q000293:injuries*; D005054:Eugenol / Q000494:pharmacology; D005260:Female; D005347:Fibroblasts / Q000187:drug effects*; D005881:Gingiva / Q000293:injuries*; D010511:Periodontal Dressings; D011145:Polyvinyls; D012117:Resins, Synthetic / Q000494:pharmacology; D014945:Wound Healing; D014950:Wounds, Penetrating / Q000628:therapy / Q000662:veterinary*
D000269:Adhesives; C073467:Barricaid; D003764:Dental Materials; C095403:Fittydent; D011145:Polyvinyls; D012117:Resins, Synthetic; D005054:Eugenol; C033389:Peripac