Activation of porcine cytomegalovirus, but not porcine lymphotropic herpesvirus, in pig-to-baboon xenotransplantation.
Tissue-invasive disease due to porcine cytomegalovirus (PCMV) has been demonstrated after pig-to-baboon solid-organ xenotransplantation. Porcine lymphotropic herpesvirus (PLHV)-1 is associated with B cell proliferation and posttransplant lymphoproliferative disorder after allogeneic bone marrow transplantation in swine but has not been observed in pig-to-primate xenotransplantation. Activation of PCMV and PLHV-1 was investigated in 22 pig-to-baboon xenotransplants by use of quantitative polymerase chain reaction. PCMV was found in all xenografts; increased viral replication occurred in 68% of xenografts during immunosuppression. PLHV-1 was found in 12 xenografts (55%); no increases in viral replication occurred during immunosuppression. Control immunosuppressed swine coinfected with PCMV and PLHV-1 had activation of PCMV but not PLHV-1. PCMV, but not PLHV-1, is activated in solid-organ xenotransplantation.
Mueller
Nicolas J
NJ
Infectious Disease Division, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA.
Livingston
Christine
C
Knosalla
Christoph
C
Barth
Rolf N
RN
Yamamoto
Shin
S
Gollackner
Bernd
B
Dor
Frank J M F
FJ
Buhler
Leo
L
Sachs
David H
DH
Yamada
Kazuhiko
K
Cooper
David K C
DK
Fishman
Jay A
JA
D000818:Animals; D030801:Animals, Genetically Modified; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000662:veterinary / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D018142:Gammaherpesvirinae / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006566:Herpesviridae Infections / Q000662:veterinary / Q000821:virology; D016377:Organ Transplantation / Q000009:adverse effects*; D010215:Papio / Q000821:virology*; D013552:Swine / Q000821:virology*; D013556:Swine, Miniature / Q000821:virology; D014183:Transplantation, Heterologous / Q000009:adverse effects*; D014775:Virus Activation
D004279:DNA, Viral