Retrotransposition of limited deletion type of intracisternal A-particle elements in the myeloid leukemia Clls of C3H/He mice.
The murine genome has about 1,000 copies of DNA elements for the intracisternal A-particle (IAP) that resembles a retrovirus. We previously reported that the genomic DNA of the cells from radiation-induced acute myeloid leukemia (AML) lines derived from C3H/He inbred mice was frequently rearranged by the integration of the IAP element. In this study, 8 IAP elements from the characteristic integration sites in 6 cell lines of radiation-induced AML from different mice were characterized and compared in structure with 114 IAP elements isolated from the normal C3H/He genome. One of the 8 elements was a full-length type I IAP, and 7 were of type-I Delta 1 with a common deletion site. Although the type I Delta 1 form is a minor population accounting for about 6% of total genomic IAP elements, it is predominantly retrotransposed in the AML cells from different C3H/He mice. This indicates that limited populations of the IAP elements contribute to the unique retrotransposition in AML cells.
Ishihara
Hiroshi
H
Redox Regulation Research Group, Radiation Safety Research Center, National Institute of Radiological Sciences, Chiba, Japan. ishihara@nirs.go.jp
Tanaka
Izumi
I
Wan
Hong
H
Nojima
Kumie
K
Yoshida
Kazuko
K
D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D017353:Gene Deletion; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D007426:Genes, Intracisternal A-Particle / Q000235:genetics* / Q000528:radiation effects*; D014644:Genetic Variation; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D007953:Leukemia, Radiation-Induced / Q000235:genetics*; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D010641:Phenotype; D018626:Retroelements / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D012689:Sequence Homology, Nucleic Acid
D018626:Retroelements