Affinity adsorbent based on combinatorial phage display peptides that bind alpha-cobratoxin.
Combinatorial phage display was used to discover peptides that selectively bind to the alpha-cobratoxin (neurotoxin) component of the multi-component venom of the Thai cobra, Naja kaouthia. Peptide sequences determined in this way were synthesized chemically and were covalently attached to agarose through the alpha-amino terminus. Such affinity chromatography supports selectively bound the alpha-cobratoxin component from crude venom, while passage of the crude venom over the support selectively depleted the venom of this component. The selective binding of alpha-cobratoxin to peptide-based solid-phase supports suggests that a limitless variety of peptides similarly obtained by combinatorial phage display can be used to craft specific analytical and preparative tools.
Byeon
Woo-Hyeon
WH
Kangwon National University, Chuncheon, 200-701, Korea.
Weisblum
Bernard
B
D000327:Adsorption; D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D002846:Chromatography, Affinity / Q000295:instrumentation*; D003039:Cobra Neurotoxin Proteins / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding
D003039:Cobra Neurotoxin Proteins; D017931:DNA Primers; D010455:Peptides; C025154:alpha-cobratoxin