Determination of some local anesthetics in human serum by gas chromatography with solid-phase extraction.
A method of analysis based on solid-phase extraction coupled with capillary gas chromatographic system for determination of mepivacaine, bupivacaine and lidocaine from human serum was developed. As extraction sorbents were used Chromosorb 103, Tenax-GC and Chromosorb T. The best extraction sorbent proved to be Chromosorb 103. Their recoveries ranged from 91 to 94% at the target concentrations of approx. 1.5 microgml(-1) in serum. Relative standard deviation of the recoveries ranged from 3.11 to 5.30 at these concentrations. As internal standard was used lidocaine. The chromatographic analysis was performed on a gas chromatograph equipped with a capillary column, HP-Innowax, and flame ionisation detector. Samples were injected in splitless mode. This method was applied in a stomatological clinic to healthy volunteers to whom superior-posterior alveolar nerve block anesthesia with mepivacaine was administered.
Baniceru
M
M
Faculty of Pharmacy, University of Medicine and Pharmacy, Peru Rares 4, 1100 Craiova, Romania. mbaniceru@k.ro
Croitoru
O
O
Popescu
S M
SM
D000328:Adult; D000368:Aged; D000779:Anesthetics, Local / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods; D005260:Female; D006801:Humans; D008875:Middle Aged; D012680:Sensitivity and Specificity; D013678:Technology, Pharmaceutical / Q000379:methods*
D000779:Anesthetics, Local