Development of a 16S rRNA primer for the detection of Brevibacterium spp.
OBJECTIVE
To develop a PCR method for the rapid identification of the genus Brevibacterium.


RESULTS
Genus-specific primers were designed by aligning and comparing the 16S sequence of all Brevibacterium spp. with closely related genera. The primer set was tested with all validly described Brevibacterium spp. and their closest neighbours.


CONCLUSIONS
Until today brevibacteria could only be identified with laborious and time-consuming phenotypic characterization. The primer from this study offers a rapid alternative to the detection of Brevibacterium spp. Brevibacteria have been isolated from food, blood, ear discharge, from a wound and from an intravascular catheter.
Gelsomino
R
R
BCCM/LMG Bacteria Culture Collection, Laboratory of Microbiology, Ghent University, Gent, Belgium. roberto.gelsomino@Ugent.be
Vancanneyt
M
M
Vandekerckhove
T M
TM
Swings
J
J
D001769:Blood / Q000382:microbiology; D001951:Brevibacterium / Q000235:genetics / Q000302:isolation & purification*; D002404:Catheterization; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000032:analysis / Q000302:isolation & purification; D004423:Ear / Q000382:microbiology; D004783:Environmental Microbiology; D005516:Food Microbiology; D020459:Genes, rRNA; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D014947:Wounds and Injuries / Q000382:microbiology
D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S