Fast EPR imaging at 300 MHz using spinning magnetic field gradients.
Electron paramagnetic resonance imaging (EPRI) technology has rapidly progressed in the last decade enabling many important applications in the fields of biology and medicine. At frequencies of 300-1200 MHz a range of in vivo applications have been performed. However, the requisite imaging time duration to acquire a given number of projections, limits the use of this technique in many in vivo applications where relatively rapid kinetics occur. Therefore, there has been a great need to develop approaches to accelerate EPRI data acquisition. We report the development of a fast low-frequency EPRI technique using spinning magnetic field gradients (SMFG). Utilizing a 300 MHz CW (continuous wave) EPRI system, SMFG enabled over 10-fold accelerated acquisition of image projections. 2D images with over 200 projections could be acquired in less than 3s and with 20s acquisitions good image quality was obtained on large aqueous free radical samples. This technique should be particularly useful for in vivo studies of free radicals and their metabolism.
Deng
Yuanmu
Y
Center for Biomedical EPR Spectroscopy and Imaging, The Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH 43210, USA.
He
Guanglong
G
Petryakov
Sergy
S
Kuppusamy
Periannan
P
Zweier
Jay L
JL
D000465:Algorithms; D004574:Electromagnetic Fields; D004578:Electron Spin Resonance Spectroscopy / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods*; D008280:Magnetics / Q000295:instrumentation*; D019047:Phantoms, Imaging; D011786:Quality Control; D011846:Radio Waves; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013113:Spin Labels
D013113:Spin Labels