Basic studies on the bone formation ability by platelet rich plasma in rabbits.
OBJECTIVE
Platelets, which contain many growth factors, such as platelet-derived growth factor (PDGF) and transforming growth factor-beta(TGF-beta), can be obtained in high concentrations by centrifugal separation and are being used as platelet-rich plasma (PRP) in clinical applications. The authors evaluated the bone formation ability by PRP in rabbits.


METHODS
In experiment 1, the authors made platelet precipitations from the whole blood obtained from rabbits. Each precipitated platelet was resuspended in fibrin glue. The glue was applied to a 1-mm wide slit defect of the same rabbit mandible. After 1 week, the authors observed the histologic appearances. In experiment 2, the fibrin glue containing the precipitated platelet was applied to a bicortical defect of a rabbit cranium with beta-tricalcium phosphate (beta-TCP) granules as artificial bone material. After 1 week, 1 month, and 2 months, the authors observed the radiologic and histologic appearances.


CONCLUSIONS
In both experiments, a prosperous bone formation was observed from the beginning more in the group treated with PRP than in the group untreated. In experiment 2, gradual appearances of the bone formation were observed in the bubble of beta-TCP. In application with artificial bone materials, bone formations by PRP were suitably observed.
Yazawa
Masaki
M
Department of Plastic and Reconstructive Surgery, Department of Pathology, and Blood Center, Keio University School of Medicine, Shinanomachi 35, Shinjuku-ku, Tokyo 160-8582, Japan. yazawa@sc.itc.keio.ac.jp
Ogata
Hisao
H
Kimura
Akiko
A
Nakajima
Tatsuo
T
Mori
Taisuke
T
Watanabe
Naohide
N
D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use; D001792:Blood Platelets / Q000502:physiology*; D001847:Bone Diseases / Q000601:surgery; D018786:Bone Substitutes / Q000627:therapeutic use; D002113:Calcification, Physiologic / Q000502:physiology; D002130:Calcium Phosphates / Q000627:therapeutic use; D002498:Centrifugation; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use; D008297:Male; D008336:Mandibular Diseases / Q000473:pathology / Q000628:therapy; D010012:Osteogenesis / Q000502:physiology*; D017713:Platelet Transfusion; D011817:Rabbits; D012886:Skull / Q000473:pathology; D013997:Time Factors; D014014:Tissue Adhesives / Q000627:therapeutic use
D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D015718:Fibrin Tissue Adhesive; D014014:Tissue Adhesives; C485817:beta-tricalcium phosphate