title: New cationic lipids for gene transfer with high efficiency and low toxicity: T-shape cholesterol ester derivatives.
abstract: New degradable cationic ester lipids with 'T-shape' configurations were synthesized and tested for gene delivery carrier. Their transfection efficiency and toxicity were compared with commercially available cationic lipids, DOTMA, DOSPA, and DC-Chol. They showed efficient transfection activity and almost no toxicity on mammalian cell lines. Their ester bond degradation was monitored by (1)H NMR.
authors.affiliation: School of Chemistry and Molecular Engineering, Seoul National University, San 56-1, Shillim-dong, Kwanak-ku, Seoul, 151-742, South Korea.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lee
authors.forename: Heebeom
authors.initials: H
authors.lastname: Koo
authors.forename: Yong-beom
authors.initials: YB
authors.lastname: Lim
authors.forename: Youngeun
authors.initials: Y
authors.lastname: Lee
authors.forename: Heejung
authors.initials: H
authors.lastname: Mo
authors.forename: Jong Sang
authors.initials: JS
authors.lastname: Park
mesh_terms: D002412:Cations; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002788:Cholesterol Esters; D004247:DNA / Q000008:administration & dosage; D006801:Humans; D008055:Lipids; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D000644:Quaternary Ammonium Compounds; D013096:Spermine / Q000031:analogs & derivatives*; D014162:Transfection / Q000379:methods* / Q000592:standards
chemical_list: C098223:2,3-dioleyloxy-N-(2-(sperminecarboxamido)ethyl)-N,N-dimethyl-1-propanaminium trifluoroacetate; D002412:Cations; D002788:Cholesterol Esters; D008055:Lipids; D000644:Quaternary Ammonium Compounds; C054005:N-(1-(2,3-dioleyloxy)propyl)-N,N,N-trimethylammonium; D013096:Spermine; D004247:DNA