Isoflavone characterization and antioxidant activity of ohio soybeans.
Seventeen Ohio soybeans were screened for isoflavone content and antioxidant activity. Isoflavone content was determined by C(18) reversed phase high-performance liquid chromatography coupled with a photodiode array detector. Antioxidant activities of soybean extracts were measured using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free radical and photochemiluminescence (PCL) methods. The highest and lowest total isoflavone contents were 11.75 and 4.20 micromol/g soy, respectively, while the average was 7.12 micromol/g soy. Antioxidant activities of soybean extracts ranged from 7.51 to 12.18 micromol butylated hydroxytoluene (BHT) equivalent/g soy using the DPPH method. Lipid and water soluble antioxidant activities of soybean extracts ranged from 2.40 to 4.44 micromol Trolox equivalent/g soy and from 174.24 to 430.86 micromol ascorbic acid equivalent/g soy, respectively, using the PCL method.
Lee
JaeHwan
J
Department of Food Science and Technology, Parker Food Science and Technology Building, The Ohio State University, 2015 Fyffe Court, Columbus, Ohio 43210, USA.
Renita
Marjory
M
Fioritto
Ronald J
RJ
St  Martin
Steve K
SK
Schwartz
Steven J
SJ
Vodovotz
Yael
Y
D000975:Antioxidants / Q000032:analysis*; D001713:Biphenyl Compounds; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000032:analysis; D007529:Isoflavones / Q000032:analysis*; D008163:Luminescent Measurements; D009820:Ohio; D010777:Photochemistry; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry*
D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D007529:Isoflavones; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl