Transformation of folate-consuming Lactobacillus gasseri into a folate producer.
Five genes essential for folate biosynthesis in Lactococcus lactis were cloned on a broad-host-range lactococcal vector and were transferred to the folate auxotroph Lactobacillus gasseri. As a result L. gasseri changed from a folate consumer to a folate producer. This principle can be used to increase folate levels in many fermented food products.
Wegkamp
Arno
A
Wageningen Centre for Food Sciences and NIZO food research, Ede, The Netherlands.
Starrenburg
Marjo
M
de Vos
Willem M
WM
Hugenholtz
Jeroen
J
Sybesma
Wilbert
W
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005492:Folic Acid / Q000096:biosynthesis* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D007778:Lactobacillus / Q000235:genetics / Q000378:metabolism*; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family; D010957:Plasmids; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005492:Folic Acid