Voltammetry of osmium-modified DNA at a mercury film electrode: application in detecting DNA hybridization.
Mercury film electrodes (MFE) have recently been used in nucleic acid electrochemical analysis as alternatives to the classical mercury drop ones. DNA modified with osmium tetroxide, 2,2'-bipyridine (Os,bipy) can be detected with a high sensitivity at mercury electrodes via measurements of a catalytic osmium signal. In this paper we show that mercury film on a glassy carbon electrode can be used in voltammetric analysis of Os,bipy-modified DNA. Application of the MFE as a detection electrode in double-surface electrochemical DNA hybridization assay involving osmium labeling of target DNA is demonstrated.
Kostecka
Pavel
P
Laboratory of Biophysical Chemistry and Molecular Oncology, Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, Brno 612 65, Czech Republic.
Havran
Ludek
L
Pivonkova
Hana
H
Fojta
Miroslav
M
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D008628:Mercury / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009942:Organometallic Compounds / Q000032:analysis / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods
D020099:Coated Materials, Biocompatible; D015342:DNA Probes; D009942:Organometallic Compounds; D015082:2,2'-Dipyridyl; C076491:osmium tetroxide-2,2'-bipyridine; D004247:DNA; C427203:calf thymus DNA; D008628:Mercury