Gene expression changes in rat white adipose tissue after a high-fat diet determined by differential display.
The differences in gene expression pattern of visceral white adipose tissue between control and high-fat-fed rats were compared using the mRNA differential display methodology. The results, confirmed by Northern blot, showed eight genes upregulated: adiponectin, fibrillin-1, transferrin, Y-box binding protein-1, IgE receptor beta chain (FcRIbeta), alpha-1 haemoglobin, and ribosomal proteins S10 and L7 and four genes downregulated: caveolin-2, lactate dehydrogenase-A, mitochondrial 16S rRNA, and mitochondrial cytochrome oxidase subunit I/serine tRNA. Two of these genes have been already related to obesity (adiponectin and caveolin-2) while the others are known to participate in metabolic, signalling or transcription regulation pathways that can be relevant in energy (lipid and/or carbohydrate) metabolism.
López
I P
IP
Department of Biochemistry and Molecular Biology, University of Navarra, 31080 Pamplona, Navarra, Spain.
Milagro
F I
FI
Martí
A
A
Moreno-Aliaga
M J
MJ
Martínez
J A
JA
De Miguel
C
C
D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D051242:Caveolin 1; D051243:Caveolin 2; D022461:Caveolins / Q000096:biosynthesis; D004041:Dietary Fats / Q000008:administration & dosage*; D015536:Down-Regulation / Q000187:drug effects; D005260:Female; D000071838:Fibrillin-1; D000071837:Fibrillins; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis; D008840:Microfilament Proteins / Q000096:biosynthesis; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation / Q000187:drug effects; D015430:Weight Gain; D054392:Adipokines
D051242:Caveolin 1; D051243:Caveolin 2; D022461:Caveolins; D004041:Dietary Fats; D000071838:Fibrillin-1; D000071837:Fibrillins; D007770:L-Lactate Dehydrogenase; D008840:Microfilament Proteins; D011506:Proteins; D012333:RNA, Messenger; C495537:Cav1 protein, rat; C000605725:Fbn1 protein, rat; D054392:Adipokines