Intramural virtual electrodes in ventricular wall: effects on epicardial polarizations.
BACKGROUND
Intramural virtual electrodes (IVEs) are believed to play an important role in defibrillation, but their existence in intact myocardium remains unproven. Here, IVEs were detected by use of optical recordings of shock-induced transmembrane potential (V(m)) changes (DeltaV(m)) measured from the intact epicardial heart surface.


RESULTS
To detect IVEs, isolated porcine left ventricles were sequentially stained with a V(m)-sensitive dye by 2 methods: (1) surface staining (SS) and (2) global staining (GS) via coronary perfusion. Shocks (2 to 50 V/cm) were applied across the ventricular wall in an epicardial-to-endocardial direction during the action potential plateau via transparent mesh electrodes, and shock-induced DeltaV(m) were measured optically from the same epicardial locations after SS and GS. Optical recordings revealed significant differences between DeltaV(m) of 2 types that became more prominent with increasing shock strength: (1) for weak shocks, SS-DeltaV(m) were larger and faster than GS-DeltaV(m); (2) for intermediate shocks, cathodal GS-DeltaV(m) became multiphasic, whereas SS-DeltaV(m) remained monophasic; and (3) for strong shocks, cathodal GS-DeltaV(m) became uniformly negative, whereas SS-DeltaV(m) typically remained positive. The radical differences in the shape and polarity of SS and GS polarizations can be explained by the contribution of subepicardial IVEs to optical signals. Histological examination revealed a dense network of collagen septa in the subepicardium, which could form the IVE substrate.


CONCLUSIONS
Intramural virtual electrodes are reflected in optical measurements of shock-induced DeltaV(m) on the intact epicardial surface. These IVEs could be a result of microscopic resistive discontinuities formed by collagen septa.
Sharifov
Oleg F
OF
Department of Biomedical Engineering, University of Alabama at Birmingham, AL 35294, USA.
Fast
Vladimir G
VG
D000200:Action Potentials; D000818:Animals; D001391:Azo Compounds / Q000032:analysis; D003094:Collagen / Q000032:analysis; D004396:Coloring Agents / Q000032:analysis; D004558:Electric Stimulation; D004566:Electrodes; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000032:analysis; D006352:Heart Ventricles / Q000737:chemistry; D008564:Membrane Potentials; D008856:Microscopy, Fluorescence; D010496:Pericardium / Q000502:physiology*; D011726:Pyridinium Compounds / Q000032:analysis; D013194:Staining and Labeling; D013552:Swine; D016276:Ventricular Function
D001391:Azo Compounds; D004396:Coloring Agents; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; C009798:C.I. direct red 80; D003094:Collagen; C050019:1-(3-sulfonatopropyl)-4-(beta)(2-(di-n-butylamino)-6-naphthylvinyl)pyridinium betaine