Application of preparative high-speed counter-current chromatography for the separation of flavonoids from the leaves of Byrsonima crassa Niedenzu (IK).
The methanolic extract of the leaves of the medicinal plant Byrsonima crassa (Malpighiaceae) contain flavonoids with antioxidant activity. They were separated in a preparative scale using high-speed counter-current chromatography. The optimum solvent system used was composed of a mixture of ethyl acetate-n-propanol-water (140:8:80 (v/v/v)) and led to a successful separation between monoglucosilated flavonoids (quercetin-3-O-alpha-L-arabinoside, quercetin-3-O-beta-D-galactoside) and the biflavonoid amentoflavone in only 3.5 h. The purities of quercetin-3-O-alpha-L-arabinoside (95 mg), quercetin-3-O-beta-D-galactoside (16 mg) and the biflavonoid amentoflavone (114 mg) were all isolated at purity over 95%. Identification was performed by 1H NMR, 13C NMR and UV analyses.
Sannomiya
Miriam
M
Departamento de Química Orgânica, Instituto de Química, UNESP, Rua Francisco Degni s/n, Bairro Quitandinha, CP 355, CEP 14800-900 Araraquara, SP, Brazil. miriamsannomiya@pop.com.br
Rodrigues
Clenilson M
CM
Coelho
Roberta G
RG
dos Santos
Lourdes C
LC
Hiruma-Lima
Clélia A
CA
Souza Brito
Alba R M
AR
Vilegas
Wagner
W
D003377:Countercurrent Distribution / Q000379:methods*; D005419:Flavonoids / Q000302:isolation & purification*; D029581:Malpighiaceae / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D005419:Flavonoids