Altered blood-brain barrier development in dystrophic MDX mice.
In order to ascertain whether the alterations of the blood-brain barrier (BBB) seen in adult dystrophic mdx-mice [Glia 42 (2003) 235], a human model of Duchenne muscular dystrophy (DMD), are developmentally established and correlated with other dystrophin isoforms which are localized at the glial-vascular interface, we used immunocytochemistry to investigate the expression of dystrophin isoforms (Dp71) during BBB development in mdx fetuses and in adult mice. Parallelly, we used Western blot, immunocytochemistry and immunogold electron microscopy to analyze the expression of the zonula occludens (ZO-1), aquaporin-4 (AQP4) and glial fibrillary acidic (GFAP) proteins as endothelial and glial markers, and we evaluated the integrity of the mdx BBB by means of intravascular injection of horseradish peroxidase (HRP). The results show reduced dystrophin isoforms (Dp71) in the mdx mouse compared with the control, starting from early embryonic life. Endothelial ZO-1 expression was reduced, and the tight junctions were altered and unlabeled. AQP4 and GFAP glial proteins in mdx mice also showed modifications in developmental expression, the glial vascular processes being only lightly AQP4- and GFAP-labeled compared with the controls. Confocal microscopy and HRP assays confirmed the alteration in vessel glial investment, GFAP perivascular endfoot reactivity being strongly reduced and BBB permeability increasing. These results demonstrate that a reduction in dystrophin isoforms (Dp71) at glial endfeet leads to an altered development of the BBB, whose no-closure might contribute to the neurological dysfunctions associated with DMD.
Nico
B
B
Department of Human Anatomy and Histology, University of Bari Medical School, Piazza Giulio Cesare, 11, Policlinico, I-70124 Bari, Italy. nico@histology.uniba.it
Paola Nicchia
G
G
Frigeri
A
A
Corsi
P
P
Mangieri
D
D
Ribatti
D
D
Svelto
M
M
Roncali
L
L
D000818:Animals; D051401:Aquaporin 4; D020346:Aquaporins / Q000096:biosynthesis; D001812:Blood-Brain Barrier / Q000254:growth & development* / Q000473:pathology* / Q000648:ultrastructure; D015153:Blotting, Western; D016189:Dystrophin / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D005333:Fetus; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000096:biosynthesis; D051379:Mice; D018101:Mice, Inbred mdx; D018613:Microscopy, Confocal; D016253:Microscopy, Immunoelectron; D009137:Muscular Dystrophy, Animal / Q000473:pathology*; D010750:Phosphoproteins / Q000096:biosynthesis; D020033:Protein Isoforms / Q000096:biosynthesis; D019108:Tight Junctions / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D062826:Zonula Occludens-1 Protein
C496031:Aqp4 protein, mouse; D051401:Aquaporin 4; D020346:Aquaporins; D016189:Dystrophin; D005904:Glial Fibrillary Acidic Protein; D008565:Membrane Proteins; D010750:Phosphoproteins; D020033:Protein Isoforms; C569042:TJP1 protein, human; C569043:Tjp1 protein, mouse; D062826:Zonula Occludens-1 Protein