title: Regulation of Cdc25C activity during the meiotic G2/M transition.
abstract: The Cdc25C phosphatase is a key activator of Cdc2/cyclin B that controls M-phase entry in eukaryotic cells. Here we discuss the regulation of Cdc25C by phosphorylation during the meiotic maturation of Xenopus oocytes. In G(2) arrested oocytes, Cdc25C is phosphorylated on Ser287 and associated with 14-3-3 proteins. Entry of the oocytes into M-phase of meiosis is triggered by progesterone, which activates a signaling pathway leading to the dephosphorylation of Ser287, probably mediated by the PP1 phosphatase. The activation of Cdc25C during oocyte maturation correlates also with its phosphorylation on multiple sites. These phosphorylations involve several signaling pathways, including Polo-like kinases and MAP kinases, and might require also the inhibition of the PP2A phosphatase. Finally, Cdc25C is further phosphorylated by its substrate Cdc2/cyclin B, as part of an auto-amplification loop that ensures the high Cdc2/cyclin B activity level required to drive the oocyte through the meiotic cell cycle.
authors.affiliation: Centro Nacional de Investigaciones Oncológicas (CNIO), Madrid, Spain.
authors.forename: Eusebio
authors.initials: E
authors.lastname: Perdiguero
authors.forename: Angel R
authors.initials: AR
authors.lastname: Nebreda
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000502:physiology*; D002455:Cell Division / Q000502:physiology*; D016195:G2 Phase / Q000502:physiology*; D006801:Humans; D008938:Mitosis / Q000502:physiology*; D020687:cdc25 Phosphatases / Q000502:physiology*
chemical_list: D018797:Cell Cycle Proteins; C517009:CDC25C protein, human; D020687:cdc25 Phosphatases