Prussian blue modified amperometric FIA biosensor: one-step immunoassay for alpha-fetoprotein.
The aim of this study was to develop a rapid method to measure alpha-fetoprotein (AFP) in human serum by use of one-step sandwich enzyme-linked immunosorbent assay (ELISA)-based immunobiosensor with disposable screen-printed carbon electrode technology. Prussian blue (PB) was deposited using cyclic voltammetry (CV) on the surface of electrode to catalyze H202 from the reaction of glucose oxidase. It took only about 30 min to complete the whole experimental procedure through flow injection analysis (FIA). A detection range obtained is in the range from 5 to 500 ng/ml AFP. This detection seems to be quick, reliable and practical.
Guan
Jian-Guo
JG
State key Lab of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, China. guanjg@public.wh.hb.cn
Miao
Yu-Qing
YQ
Chen
Jian-Rong
JR
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001774:Blood Chemical Analysis / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005295:Ferrocyanides / Q000737:chemistry*; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000509:alpha-Fetoproteins / Q000032:analysis*
D020099:Coated Materials, Biocompatible; D005295:Ferrocyanides; D000509:alpha-Fetoproteins; C000170:ferric ferrocyanide