Metabolomic differential display analysis of the white-rot basidiomycete Phanerochaete chrysosporium grown under air and 100% oxygen.
When the cultural atmosphere of the white-rot basidiomycete, Phanerochaete chrysosporium, was changed from air to 100% oxygen, the lyophilized mycelial weight increased and thickening of extracellular glucan layer was observed in 2-3 days. To better understand the oxygen-stress responsive mechanism of P. chrysosporium, the metabolomic differential display analysis was performed using metabolites isolated from fungal cells grown under either air or 100% O(2) atmosphere. In the GC-MS total ion chromatogram of methanol-extracts from fungal cells, at least 183 peaks were detected and 53 compounds were identified. Among them, veratryl alcohol (VA), threonate, and erythronate were identified as oxygen-stress responsive metabolites. The intracellular concentration of VA increased dramatically within 1 h after an oxygen purge, indicating that VA production is sensitive to the oxygen stress in P. chrysosporium.
Miura
Daisuke
D
Department of Forest and Forest Products Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Tanaka
Hiroo
H
Wariishi
Hiroyuki
H
D000388:Air; D001592:Benzyl Alcohols / Q000032:analysis / Q000302:isolation & purification; D018533:Biomass; D002087:Butyrates / Q000032:analysis / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry; D005936:Glucans / Q000096:biosynthesis / Q000378:metabolism; D025282:Mycelium / Q000254:growth & development / Q000378:metabolism; D018384:Oxidative Stress; D010100:Oxygen; D020075:Phanerochaete / Q000737:chemistry* / Q000378:metabolism*
D001592:Benzyl Alcohols; D002087:Butyrates; D005936:Glucans; C121691:erythronic acid; C042197:veratryl alcohol; C011369:threonic acid; D010100:Oxygen