Inhibition of PMN apoptosis after adherence to dip-coated calcium phosphate surfaces on a NiTi shape memory alloy.
Nickel-titanium shape-memory alloys (NiTi-SMA) were coated with calcium phosphate by dipping in oversaturated calcium phosphate solution (CaP-coating). Polymorphonuclear neutrophil granulocytes (PMN) belong to the first cells which will adhere to implant materials. We analyzed the apoptosis of isolated human PMN after cell culture on non-coated and CaP-coated NiTi-SMA by light and scanning electron microscopy (cell morphology) and by flow cytometry (DNA-fragmentation). In contrast to PMN adherent to non-coated NiTi-SMA, the apoptosis of PMN adherent CaP-coated samples was inhibited. Cell culture media obtained from cultured leukocytes with CaP-coatings (conditioned media, CM) were able to transfer the apoptosis inhibiting activities to freshly isolated PMN. There was a significant (p<0.01) increase in GM-CSF, IL-8, IL-6, and TNF-alpha within CM obtained from coated versus non-coated NiTi-SMA as was determined by ELISA.
Bogdanski
Denise
D
Department of Surgery, Surgical Research, BG Kliniken Bergmannsheil-Universitätsklinik, Bürkle-de-la-Camp-Platz 1, D-44780 Bochum, Germany.
Esenwein
Stefan A
SA
Prymak
Oleg
O
Epple
Matthias
M
Muhr
Gert
G
Köller
Manfred
M
D000327:Adsorption; D017209:Apoptosis / Q000502:physiology; D002130:Calcium Phosphates / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D009504:Neutrophils / Q000166:cytology* / Q000502:physiology*; D009532:Nickel / Q000737:chemistry*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; C040654:titanium nickelide; D009532:Nickel; C020243:calcium phosphate; D014025:Titanium