Interleukin-8 response in an intestinal HCT-8 cell line infected with enteroaggregative and enterotoxigenic Escherichia coli.
This study examined the interleukin-8 (IL-8) response of the intestinal adenocarcinoma HCT-8 cell line to infection with enteroaggregative and enterotoxigenic Escherichia coli pathotypes isolated from patients with travelers' diarrhea. Individual diarrheagenic E. coli strains (enteroaggregative E. coli [EAEC]; n = 30), heat-stable enterotoxin (ST)-producing enterotoxigenic E. coli (ETEC ST; n = 11), heat-labile enterotoxin (LT)-producing enterotoxigenic E. coli (ETEC LT; n = 10), and ST- and LT-producing enterotoxigenic E. coli (ETEC ST:LT; n = 8) were coincubated with HCT-8 cells for 3 h. Tissue culture supernatants were assayed for IL-8 content by enzyme-linked immunosorbent assay. Fifty percent of EAEC (72% of those EAEC carrying the virulence factors aggR, aggA, and aspU and 40% of those EAEC not carrying virulence factors) and 64% of ETEC ST elicited IL-8 production. In contrast, 10% of ETEC LT elicited the production of IL-8 above baseline. These results suggest that (i) the HCT-8 cell line infection model can be used as a tool to differentiate proinflammatory E. coli from noninflammatory isolates; (ii) EAEC has a heterogeneous ability to induce the production of IL-8, and this may be associated with the presence of virulence factors; and (iii) ETEC ST can elicit an inflammatory response and helps explain our earlier findings of increased fecal IL-8 in patients with ETEC diarrhea.
Huang
David B
DB
University of Texas Health Science Center, Houston Medical School, 6431 Fannin, JFB 1.728, Houston, TX 77030, USA.
DuPont
Herbert L
HL
Jiang
Zhi-Dong
ZD
Carlin
Lily
L
Okhuysen
Pablo C
PC
D000230:Adenocarcinoma / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D001427:Bacterial Toxins / Q000235:genetics; D045744:Cell Line, Tumor; D004269:DNA, Bacterial / Q000235:genetics; D004768:Enterotoxins / Q000235:genetics / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000235:genetics / Q000502:physiology; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D007414:Intestinal Neoplasms / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D016133:Polymerase Chain Reaction; D014774:Virulence / Q000235:genetics
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D016209:Interleukin-8; C034151:heat stable toxin (E coli); C027538:heat-labile enterotoxin, E coli