title: An in vitro transcription system for studying vaccinia virus late genes.
abstract: This chapter describes a protocol that allows accurate in vitro transcription of vaccinia virus late genes. In this method, extracts are made from vaccinia virus-infected cells and used as enzyme sources to produce mRNAs from plasmid templates containing late gene promoter sequences.
authors.affiliation: Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC, USA.
authors.forename: Cynthia F
authors.initials: CF
authors.lastname: Wright
mesh_terms: D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D066298:In Vitro Techniques; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D014158:Transcription, Genetic / Q000235:genetics*; D014616:Vaccinia virus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins