title: Identification of a collagen production-promoting factor from an extract of royal jelly and its possible mechanism.
abstract: We have previously shown that royal jelly (RJ) promoted collagen production by skin fibroblasts in the presence of ascorbic acid-2-O-alpha-glucoside (AA-2G). In this study, we purified the honeybee RJ-derived collagen production-promoting factor (HBRJ-CPF) from an alkali-solubilized fraction of RJ by C18 reverse-phase column chromatography. The elution profile by the C18 column chromatography and the molecular mass of the purified HBRJ-CPF material coincided with those of 10-hydroxy-2-decenoic acid (10H2DA). We then examined the collagen production-promoting activities of several commercially available fatty acids contained in RJ. We found that 10H2DA and 10-hydroxydecanoic acid increased the collagen production in a dose-dependent manner. Furthermore, 10H2DA induced the fibroblast cell line, NHDF, to produce transforming growth factor-beta 1 (TGF-beta 1) which is an important factor for collagen production. As expected, the collagen production-promoting activity of 10H2DA was neutralized by the anti-TGF-beta 1 antibody. These result suggest that HBRJ-CPF identified as 10H2DA promoted the collagen production of AA-2G-treated fibroblasts by inducing TGF-beta 1 production.
authors.affiliation: Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc., Fujisaki, Okayama, Japan. fujisaki@hayashibara.co.jp
authors.forename: Satomi
authors.initials: S
authors.lastname: Koya-Miyata
authors.forename: Iwao
authors.initials: I
authors.lastname: Okamoto
authors.forename: Shimpei
authors.initials: S
authors.lastname: Ushio
authors.forename: Kanso
authors.initials: K
authors.lastname: Iwaki
authors.forename: Masao
authors.initials: M
authors.lastname: Ikeda
authors.forename: Masashi
authors.initials: M
authors.lastname: Kurimoto
mesh_terms: D000468:Alkalies / Q000737:chemistry; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000096:biosynthesis*; D005227:Fatty Acids / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012995:Solubility; D016212:Transforming Growth Factor beta / Q000378:metabolism; D000099219:Royal Jelly
chemical_list: D000468:Alkalies; D003094:Collagen; D005227:Fatty Acids; D016212:Transforming Growth Factor beta; D000099219:Royal Jelly