title: Cytogenetic abnormalities and the failure of development after round spermatid injections.
abstract: OBJECTIVE
  To assess cytologic and cytogenetic abnormalities following round spermatid injection.
  
  
  METHODS
  Prospective analysis.
  
  
  METHODS
  In vitro fertilization centers.
  
  
  METHODS
  Fourteen couples accepted to a round spermatid injection (ROSI) and preimplantation genetic diagnosis (PGD) program after appropriate counseling.
  
  
  METHODS
  ROSI, PGD, with fluorescence in situ hybridization for chromosome enumeration.
  
  
  METHODS
  Cytologic and cytogenetic abnormalities in oocytes, zygotes, and blastomeres.
  
  
  RESULTS
  The fertilization rate following ROSI was 36%. Only 11 of 143 (7.7%) oocytes developed to have several blastomeres. Cytologic and cytogenetic abnormalities accounted for the vast majority of blockage at oocyte, zygote, and early mitotic division stages. Four biopsied embryos were normal. These and seven others were implanted, but no pregnancy was achieved.
  
  
  CONCLUSIONS
  A PGD diagnosis for common aneuploidies and blastocyst stage transfer is feasible for ROSI cases. Failure with ROSI is cause primarily by chromosome abnormalities, so use of ROSI in assisted reproductive technologies should be limited.
authors.affiliation: ART and Reproductive Genetics Unit, Istanbul Memorial Hospital, Istanbul, Turkey. atl78@aol.com
authors.forename: Moncef
authors.initials: M
authors.lastname: Benkhalifa
authors.forename: Semra
authors.initials: S
authors.lastname: Kahraman
authors.forename: Annil
authors.initials: A
authors.lastname: Biricik
authors.forename: Semra
authors.initials: S
authors.lastname: Serteyl
authors.forename: Ersan
authors.initials: E
authors.lastname: Domez
authors.forename: Yacoup
authors.initials: Y
authors.lastname: Kumtepe
authors.forename: Mazin B
authors.initials: MB
authors.lastname: Qumsiyeh
mesh_terms: D002869:Chromosome Aberrations; D005314:Embryonic and Fetal Development; D005260:Female; D005306:Fertilization; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007267:Injections; D008297:Male; D011446:Prospective Studies; D013087:Spermatids / Q000648:ultrastructure*