title: Compositional equivalency of Cry1F corn event TC6275 and conventional corn (Zea mays L.).
abstract: Maize (Zea mays L.) plants have been transformed to express a Cry1F insecticidal crystal protein originally isolated from Bacillus thuringiensis Berliner. This protein controls lepidopteran pests of maize, including the European corn borer, Ostrinia nubilalis (Hübner). As part of the safety assessment for crops containing transgenes, a compositional analysis of the food and feed is conducted. This analysis is designed to detect unintended changes in the nutrient and antinutrient content of the raw commodities produced by the crop due to the insertion of the genes into the genomic DNA of the plant (pleotropic effects). Samples of transgenic and nontransgenic maize forage and grain were collected from six field sites located in the U.S. and Canada. Forage samples were analyzed for proximates and minerals, and grain was further analyzed for fatty acids, amino acids, vitamins, secondary metabolites, and antinutrients. Results demonstrated that maize expressing the Cry1F protein was equivalent to nontransgenic maize with respect to these important components. Comparison of the variability within the nontransgenic and transgenic hybrid, as compared to composition values reported in the literature, suggest that factors other than transgenes may contribute more substantially to the composition of crops.
authors.affiliation: Dow AgroSciences LLC, 9330 Zionsville Road, Indianapolis, Indiana 46268, USA. raherman@dow.com
authors.forename: Rod A
authors.initials: RA
authors.lastname: Herman
authors.forename: Amy M
authors.initials: AM
authors.lastname: Phillips
authors.forename: Randy A
authors.initials: RA
authors.lastname: Collins
authors.forename: Laura A
authors.initials: LA
authors.lastname: Tagliani
authors.forename: Fred A
authors.initials: FA
authors.lastname: Claussen
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Graham
authors.forename: Brenda L
authors.initials: BL
authors.lastname: Bickers
authors.forename: Travis A
authors.initials: TA
authors.lastname: Harris
authors.forename: Lee M
authors.initials: LM
authors.lastname: Prochaska
mesh_terms: D000601:Amino Acids, Essential / Q000032:analysis; D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins; D004731:Endotoxins / Q000235:genetics; D006460:Hemolysin Proteins; D007915:Lepidoptera; D008903:Minerals / Q000032:analysis; D010572:Pest Control, Biological; D030821:Plants, Genetically Modified / Q000737:chemistry*; D014815:Vitamins / Q000032:analysis; D003313:Zea mays / Q000737:chemistry* / Q000235:genetics*
chemical_list: D000601:Amino Acids, Essential; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D008903:Minerals; D014815:Vitamins; C030956:insecticidal crystal protein, Bacillus Thuringiensis