Golgi duplication in Trypanosoma brucei.
Duplication of the single Golgi apparatus in the protozoan parasite Trypanosoma brucei has been followed by tagging a putative Golgi enzyme and a matrix protein with variants of GFP. Video microscopy shows that the new Golgi appears de novo, near to the old Golgi, about two hours into the cell cycle and grows over a two-hour period until it is the same size as the old Golgi. Duplication of the endoplasmic reticulum (ER) export site follows exactly the same time course. Photobleaching experiments show that the new Golgi is not the exclusive product of the new ER export site. Rather, it is supplied, at least in part, by material directly from the old Golgi. Pharmacological experiments show that the site of the new Golgi and ER export is determined by the location of the new basal body.
He
Cynthia Y
CY
Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar St., P.O. Box 208002, New Haven, CT 06520-8002, USA.
Ho
Helen H
HH
Malsam
Joerg
J
Chalouni
Cecile
C
West
Christopher M
CM
Ullu
Elisabetta
E
Toomre
Derek
D
Warren
Graham
G
D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D002478:Cells, Cultured; D004721:Endoplasmic Reticulum / Q000378:metabolism / Q000648:ultrastructure; D005407:Flagella / Q000378:metabolism / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006056:Golgi Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D008565:Membrane Proteins / Q000378:metabolism; D008854:Microscopy, Electron; D018715:Microscopy, Video; D021381:Protein Transport / Q000502:physiology; D015800:Protozoan Proteins / Q000378:metabolism; D014346:Trypanosoma brucei brucei / Q000378:metabolism* / Q000648:ultrastructure
D008164:Luminescent Proteins; D008565:Membrane Proteins; D015800:Protozoan Proteins; D049452:Green Fluorescent Proteins