Immunofluorescence assay for serologic diagnosis of SARS.
We evaluated an indirect immunofluorescence assay based on virus-infected cells for detecting anti-severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.
Chan
Paul K S
PK
Department of Microbiology, Faculty of Medicine, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, People's Republic of China. paulkschan@cuhk.edu.hk
Ng
King-Cheung
KC
Chan
Rickjason C W
RC
Lam
Rebecca K Y
RK
Chow
Viola C Y
VC
Hui
Mamie
M
Wu
Alan
A
Lee
Nelson
N
Yap
Florence H Y
FH
Cheng
Frankie W T
FW
Sung
Joseph J Y
JJ
Tam
John S
JS
D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D021821:Communicable Diseases, Emerging / Q000175:diagnosis* / Q000276:immunology / Q000503:physiopathology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D006723:Hong Kong; D006801:Humans; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045169:Severe Acute Respiratory Syndrome / Q000175:diagnosis* / Q000276:immunology / Q000503:physiopathology
D000914:Antibodies, Viral