[The effects of the tangfukang capsules on cytokines of early diabetic nephropathy].
OBJECTIVE
To find out the mechanism of Tangfukang on the diabetic nephropathy(DN).


METHODS
A Model of streptorotocin diabetic rats was used. The expressions of the AGEs, ICAM-1, IV-C and FN were observed in renal cortex of diabetic rats with immunohistochemical method. The level of the IL-1 beta in blood serum was measured by radioimmunoassay method and the level of TNF was determined in blood serum by ELISA method. Aminoguanidine was selected as the control medicine which could inhibit the expression of monenzymatic glycosylation end products of protein. All the results were compared to analyze the effect of Tangfukang on monenzymatic glycosylation of protein and cytokine expression in early diabetic nephropathy rats.


RESULTS
Both Tangfukang and aminoguanidine significantly decreased the expression of AGEs, but Tangfukang was greatly superior to aminoguanidine in suppressing the expression of cytokine like ICAM-1, IL-1 beta, TNF-alpha and the ingredients of extracellular matrix like IV-C, FN.


CONCLUSIONS
It may be the partical mechanism of Tangfukang to suppress the monenzymatic glycosylation of protein and to reduce the expression of cytokine in diabetes. The effect of Tangfukang is superior to that of aminoguanidine.
Zhang
Xue-zhu
XZ
Department of Biochem. Eng., School of Chem. Eng. & Tech. Tianjin University, Tianjin 300072, China.
Zhao
Kai-li
KL
Dai
Qi
Q
Li
Lin
L
Zhang
Bai-lin
BL
D000302:Adrenal Cortex / Q000378:metabolism; D000818:Animals; D027885:Astragalus propinquus / Q000737:chemistry; D002214:Capsules; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D003928:Diabetic Nephropathies / Q000378:metabolism*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007375:Interleukin-1 / Q000097:blood; D031659:Ligustrum / Q000737:chemistry; D008297:Male; D032102:Phellodendron / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D002214:Capsules; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D017127:Glycation End Products, Advanced; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1