title: Differential expression of three plastidial sigma factors, OsSIG1, OsSIG2A, and OsSIG2B, during leaf development in rice.
abstract: We isolated and characterized two rice nuclear genes, OsSIG2A and OsSIG2B, encoding the putative sigma-factor of the plastid RNA polymerase. Deduced protein sequences predicted a plastid-localizing signal in the N-terminus and subsequent polypeptides similar to known SIG2 proteins. Gene expression analysis revealed that the OsSIG2A transcript is more abundant than the OsSIG2B transcript in all tissues tested and that both rice SIG2s are expressed from earlier stages of leaf development than that in the case of OsSIG1. These results indicate differential expression of SIG genes in leaf morphogenesis, suggesting the existence of tissue- and stage-specific functions of SIG proteins for transcriptional regulation of chloroplast genes in plant development.
authors.affiliation: National Food Research Institute, Tsukuba, Ibaraki, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Kasai
authors.forename: Makiko
authors.initials: M
authors.lastname: Kawagishi-Kobayashi
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Teraishi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Kozo
authors.initials: K
authors.lastname: Ochi
authors.forename: Kyo
authors.initials: K
authors.lastname: Wakasa
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Tozawa
mesh_terms: D000595:Amino Acid Sequence; D018506:Gene Expression Regulation, Plant; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry / Q000166:cytology / Q000235:genetics* / Q000254:growth & development*; D010802:Phylogeny; D018515:Plant Leaves / Q000737:chemistry / Q000166:cytology / Q000235:genetics* / Q000254:growth & development*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics; D018087:Plastids / Q000235:genetics* / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D012808:Sigma Factor / Q000737:chemistry / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D018749:RNA, Plant; D012808:Sigma Factor