title: Global analysis of gene expression in an rpoN mutant of Listeria monocytogenes.
abstract: The role of the alternative sigma(54) factor, encoded by the rpoN gene, was investigated in Listeria monocytogenes by comparing the global gene expression of the wild-type EGDe strain and an rpoN mutant. Gene expression, using whole-genome macroarrays, and protein content, using two-dimensional gel electrophoresis, were analysed. Seventy-seven genes and nine proteins, whose expression was modulated in the rpoN mutant as compared to the wild-type strain, were identified. Most of the modifications were related to carbohydrate metabolism and in particular to pyruvate metabolism. However, under the conditions studied, only the mptACD operon was shown to be directly controlled by sigma(54). Therefore, the remaining modifications seem to be due to indirect effects. In parallel, an in silico analysis suggests that sigma(54) may directly control the expression of four different phosphotransferase system (PTS) operons, including mptACD. PTS activity is known to have a direct effect on the pyruvate pool and on catabolite regulation. These results suggest that sigma(54) is mainly involved in the control of carbohydrate metabolism in L. monocytogenes via direct regulation of PTS activity, alteration of the pyruvate pool and modulation of carbon catabolite regulation.
authors.affiliation: Equipe de Microbiologie Fondamentale et Appliquée, Université de Poitiers, 40 avenue du Recteur Pineau, 86022 Poitiers Cedex, France.
authors.forename: Safia
authors.initials: S
authors.lastname: Arous
authors.affiliation: Laboratoire de Génomique des Micro-organismes Pathogènes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France.
authors.forename: Carmen
authors.initials: C
authors.lastname: Buchrieser
authors.affiliation: Station de Recherches sur la Viande, Institut National de la Recherche Agronomique de Theix, 63122 Saint-Genes Champanelle, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Folio
authors.affiliation: Laboratoire de Génomique des Micro-organismes Pathogènes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Glaser
authors.affiliation: Plateforme de protéomique, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France.
authors.forename: Abdelkader
authors.initials: A
authors.lastname: Namane
authors.affiliation: Station de Recherches sur la Viande, Institut National de la Recherche Agronomique de Theix, 63122 Saint-Genes Champanelle, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Hébraud
authors.affiliation: Equipe de Microbiologie Fondamentale et Appliquée, Université de Poitiers, 40 avenue du Recteur Pineau, 86022 Poitiers Cedex, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Héchard
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D050260:Carbohydrate Metabolism; D004268:DNA-Binding Proteins; D012321:DNA-Directed RNA Polymerases / Q000235:genetics* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D008089:Listeria monocytogenes / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011401:Promoter Regions, Genetic; D051877:RNA Polymerase Sigma 54; D012329:RNA, Bacterial / Q000378:metabolism; D012808:Sigma Factor / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012329:RNA, Bacterial; D012808:Sigma Factor; D012321:DNA-Directed RNA Polymerases; D051877:RNA Polymerase Sigma 54