Rapid detection and differentiation method of VanA, VanB and VanC phenotypes in vancomycin-resistant enterococci.
We developed a simple method that can replace the polymerase chain reaction (PCR) to distinguish between vancomycin-resistant enterococci (VRE) with the vanA, vanB and vanC genes. The method is based on induction of teicoplanin resistance by vancomycin in vanB-VRE, while the two compounds have a synergistic effect in vanC-VRE. In addition, vanA-VRE shows resistance to both vancomycin and teicoplanin, and both the compounds can induce resistance to vanA-VRE. Utilising these properties, we attempted to develop a simple method to distinguish between vanA, vanB and vanC. We compared our simple method with the PCR method in 43 strains of vanA-VRE, 35 strains of vanB-VRE and 37 strains of vanC-VRE. The results were 100% consistent with that obtained by PCR.
Hanaki
Hideaki
H
Laboratory of Clinical Bacteriology, Department Nutrition Nakamuragakuen University, 5-7-1 Befu, Jyonan-ku, Fukuoka, Japan. hanaki@lisci.kitasato-u.ac.jp
Yamaguchi
Yoshio
Y
Nomura
Syuichi
S
Nagayama
Ariaki
A
Sunakawa
Keisuke
K
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D019729:Carbon-Oxygen Ligases / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis; D004357:Drug Synergism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008826:Microbial Sensitivity Tests; D010453:Peptide Synthases / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D016133:Polymerase Chain Reaction; D013291:Streptococcus / Q000187:drug effects* / Q000235:genetics; D017334:Teicoplanin / Q000494:pharmacology; D014640:Vancomycin / Q000494:pharmacology; D020713:Vancomycin Resistance / Q000235:genetics*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C071437:VanA ligase, Bacteria; C079913:VanB protein, Enterococcus; D017334:Teicoplanin; D014640:Vancomycin; D019729:Carbon-Oxygen Ligases; D010453:Peptide Synthases; C108395:VanC protein, Enterococcus