title: Neuronal traits are required for glucose-induced insulin secretion.
abstract: The transcriptional repressor RE1 silencer transcription factor (REST) is an important factor that restricts some neuronal traits to neurons. Since these traits are also present in pancreatic beta-cells, we evaluated their role by generating a model of insulin-secreting cells that express REST. The presence of REST led to a decrease in expression of its known target genes, whereas insulin expression and its cellular content were conserved. As a consequence of REST expression, the capacity to secrete insulin in response to mitochondrial fuels, a particularity of mature beta-cells, was impaired. These data provide evidence that REST target genes are required for an appropriate glucose-induced insulin secretion.
authors.affiliation: Department of Internal Medicine, University of Lausanne, CHUV-1011 Lausanne, Switzerland.
authors.forename: Amar
authors.initials: A
authors.lastname: Abderrahmani
authors.forename: Guy
authors.initials: G
authors.lastname: Niederhauser
authors.forename: Valérie
authors.initials: V
authors.lastname: Plaisance
authors.forename: Jacques-Antoine
authors.initials: JA
authors.lastname: Haefliger
authors.forename: Romano
authors.initials: R
authors.lastname: Regazzi
authors.forename: Gérard
authors.initials: G
authors.lastname: Waeber
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D056970:Co-Repressor Proteins; D004396:Coloring Agents / Q000494:pharmacology; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism*; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013997:Time Factors; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D056970:Co-Repressor Proteins; D004396:Coloring Agents; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D007328:Insulin; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C485650:Rcor2 protein, mouse; D012097:Repressor Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; D012313:RNA; D011189:Potassium Chloride; C022616:thiazolyl blue; D005947:Glucose