title: N1-dansyl-spermine: a potent polyamine antagonist.
abstract: The potential polyamine antagonist action of N1-dansyl-spermine (a potent NMDA antagonist) was assessed in two in vivo mouse models of polyamine action. Co-administration of N1-dansyl-spermine (2-10 microg, i.c.v.) with spermine (100 microg, i.c.v.) resulted in a dose-dependent antagonism of the spermine-induced CNS excitation (body tremor and fatal tonic convulsions). In addition, the same dose of N1-dansyl-spermine antagonised spermine's enhancement of NMDA-induced convulsions. These results suggest that N1-dansyl-spermine is in vivo a potent antagonist of the CNS effects of spermine and of its action at the positive polyamine modulatory site on the NMDA receptor.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Trinity College, Dublin 2, Ireland. bkirby@rcsi.ie
authors.forename: Brian P
authors.initials: BP
authors.lastname: Kirby
authors.forename: Sheila A
authors.initials: SA
authors.lastname: Ryder
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Seiler
authors.forename: Jacques
authors.initials: J
authors.lastname: Renault
authors.forename: Graham G
authors.initials: GG
authors.lastname: Shaw
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000187:drug effects* / Q000503:physiopathology; D003619:Dansyl Compounds / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D005260:Female; D007276:Injections, Intraventricular; D051379:Mice; D016202:N-Methylaspartate / Q000494:pharmacology; D011073:Polyamines / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D011930:Reaction Time / Q000187:drug effects; D012640:Seizures / Q000139:chemically induced / Q000188:drug therapy; D013096:Spermine / Q000031:analogs & derivatives* / Q000494:pharmacology*
chemical_list: D003619:Dansyl Compounds; D018690:Excitatory Amino Acid Agonists; C105932:N(1)-dansylspermine; D011073:Polyamines; D013096:Spermine; D016202:N-Methylaspartate