[The effect of calcium ionophore on dendritic cells derived from peripheral blood mononuclear cells].
OBJECTIVE
To explore the effect of calcium ionophore (CI) on dendritic cells (DC) derived from peripheral blood monocytes.


METHODS
Peripheral blood monocytes from healthy donors were treated with 100 microg/L rhGM-CSF, 100 microg/L rhGM-CSF plus (10 microg/L) CI, and 100 microg/L rhGM-CSF plus (100 microg/L) respectively. After cultivation of 40 hours, cellular morphology was observed under light microscope and electron microscope. Surface markers on treated PBMCs were analyzed by flow cytometry. MTT colorimetry was used to detect proliferation of autologous T cells.


RESULTS
Peripheral blood monocytes treated with 100 microg/L rhGM-CSF plus 100 microg/L CI for 40 hours showed typical morphology of DCs. Simultaneously there was a decrease in CD14 expression and increase in HLA-DR, CD40, CD83 and CD86 expressions on these cells. In addition, PBMCs treated with 100 microg/L rhGM-CSF pass 100 microg/L CI for 40 hrs. Could evidently stimulate proliferation of autologous T cells.


CONCLUSIONS
CI can markedly enhance transformation of peripheral blood monocytes induced by GM-CSF into DCs.
Wu
Jun
J
Institute of Molecular Oncology, General Hospital of Guangzhou Command, Guangzhou 510010, China. Junwuwang@hotmail.com
Wang
Xiao-huai
XH
Wang
Jie
J
Yang
Tai-cheng
TC
Lai
Huang-wen
HW
Zheng
Wen-ling
WL
D015703:Antigens, CD / Q000378:metabolism*; D051940:B7-2 Antigen / Q000378:metabolism; D019013:CD40 Antigens / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism*; D007476:Ionophores / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism*; D011994:Recombinant Proteins; D013601:T-Lymphocytes / Q000166:cytology; D000097802:CD83 Antigen
D015703:Antigens, CD; D051940:B7-2 Antigen; D019013:CD40 Antigens; D002118:Calcium; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006684:HLA-DR Antigens; D007136:Immunoglobulins; D007476:Ionophores; D018950:Lipopolysaccharide Receptors; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D000097802:CD83 Antigen