[Effects of NGF and estrogens on human hair follicle in vitro].
OBJECTIVE
To observe the effects of NGF, estrogens and minoxidil on the growth of human hair follicle in vitro.


METHODS
In a model of human hair follicle in vitro, the follicle was separately treated with the NGF, estrogens and minoxidil. The growth of the hair follicle was measured in length with an eyepiece micrometer. The effects of the NGF, estrogens and minoxidil were evaluated by measuring the rates of incorporation of 3H-TdR of DNA synthesis.


RESULTS
The growth of the human hair follicle was showing significantly faster in the 100 ng/ml NGF and 125 micrograms/ml minoxidil groups, compared with the control (P < 0.05), but the growth was significantly inhibited in the 0.5 microgram/ml 17 beta-E2 group (P < 0.05). There was no difference shown for the growth of the hair follicle in the group mixed with 100 ng/ml NGF and 0.5 microgram/ml 17 beta-E2 (P > 0.05). The rates of incorporation of 3H-TdR in the groups were shown that the results just correlated with the results of the above-mentioned method.


CONCLUSIONS
The 100 ng/ml NGF and 125 micrograms/ml minoxidil could increase the growth of human hair follicle while the 0.5 microgram/ml 17 beta-E2 could inhibit it. The 100 ng/ml NGF could neutralized the effect of the 0.5 microgram/ml 17 beta-E2.
Yang
Zhuang-qun
ZQ
Department of Oral and Maxillary Surgery, Stomatological College, Xi'an Jiao Tong University, Xi'an 710004, China.
Tu
Jun-bo
JB
Yao
Tian-hua
TH
Zhao
Xiao-ge
XG
D000293:Adolescent; D000328:Adult; D004967:Estrogens / Q000494:pharmacology*; D018859:Hair Follicle / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D066298:In Vitro Techniques; D008875:Middle Aged; D008914:Minoxidil / Q000494:pharmacology; D020932:Nerve Growth Factor / Q000494:pharmacology*; D014665:Vasodilator Agents / Q000494:pharmacology
D004967:Estrogens; D014665:Vasodilator Agents; D008914:Minoxidil; D020932:Nerve Growth Factor