Ion transport across Xenopus alveolar epithelium is regulated by extracellular ATP, UTP and adenosine.
Native alveolar epithelium from Xenopus lung was used for electrophysiological Ussing chamber experiments to investigate ion transport regulation. The tissue exhibits a considerable absorption of Na(+) ions and this transepithelial transport is largely up-regulated after treatment of donor animals with ACTH. Extracellular ATP, UTP and adenosine were tested for their regulating effects and all three increased I(sc), which was mainly due to a stimulation of amiloride sensitive Na(+) transport (increase of I(ami) 32% for ATP, 21% for UTP, 25% for adenosine). Solely the effect of UTP was completely abolished in the presence of amiloride. In contrast, the effects of ATP or adenosine disappeared under Cl(-)-free conditions. ATP and UTP proved to have additive effects and pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid (PPADS), an antagonist of purinergic receptors, inhibited selectively the effect of UTP on I(sc). Further, I(sc) was increased by the P2X selective agonist beta,gamma-meATP. We were able to demonstrate, that extracellular purines and pyrimidines play a possible role as auto/paracrine messengers for alveolar ion transport regulation in Xenopus lung.
Fronius
Martin
M
Institute of Animal Physiology, Justus-Liebig-University Giessen, Wartweg 95, D-35392 Giessen, Germany. martin.fronius@bio.uni-giessen.de
Berk
Andreas
A
Clauss
Wolfgang
W
Schnizler
Mikael
M
D000241:Adenosine / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D004347:Drug Interactions; D005110:Extracellular Space / Q000187:drug effects; D005260:Female; D017136:Ion Transport / Q000187:drug effects*; D008564:Membrane Potentials / Q000187:drug effects; D018408:Patch-Clamp Techniques / Q000379:methods; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D014544:Uridine Triphosphate / Q000494:pharmacology*; D014982:Xenopus laevis
D010975:Platelet Aggregation Inhibitors; C077792:pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid; D011732:Pyridoxal Phosphate; D000255:Adenosine Triphosphate; D000324:Adrenocorticotropic Hormone; D012964:Sodium; D000241:Adenosine; D014544:Uridine Triphosphate