The T cell activation marker CD150 can be used to identify alloantigen-activated CD4(+)25+ regulatory T cells.
We have been investigating whether alloantigen-specific CD4(+)25+ regulatory T cells can be identified for use in treating graft-versus-host disease. CD150, which is upregulated on the surface of all activated T lymphocytes, was identified as a candidate marker for alloantigen-activated CD4(+)25+ regulatory T cells by gene chip analysis. Freshly isolated CD4(+)25+ cells had only low cell-surface expression of CD150, comparable to that of CD4(+)25- T cells. Increased CD150 expression was observed on all T cells after coculture with allogeneic stimulator cells. When purified CD4(+)25+ cells were precultured with allogeneic stimulator cells, then sorted into CD150+ and CD150- subsets, allosuppressive activity was contained primarily in the CD150+ fraction. These cells also suppressed the proliferation of alloantigen-activated autologous T cells, and they could be expanded in vitro without loss of their suppressive capacity. These results suggest that CD150 can be used as a marker for the identification of purified alloantigen-activated CD4(+)25+ regulatory T cells.
Browning
Meghen B
MB
Department of Pediatrics, The Medical College of Wisconsin and the Children's Research Institute of the Children's Hospital of Wisconsin, Milwaukee, WI 53226, USA. mbrownin@mcw.edu
Woodliff
Jeffrey E
JE
Konkol
Marja C
MC
Pati
Nirupma T
NT
Ghosh
Soumitra
S
Truitt
Robert L
RL
Johnson
Bryon D
BD
D015703:Antigens, CD; D015704:CD4 Antigens / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis*; D007519:Isoantigens / Q000276:immunology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D011948:Receptors, Antigen, T-Cell / Q000502:physiology; D011956:Receptors, Cell Surface; D015375:Receptors, Interleukin-2 / Q000032:analysis*; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D013601:T-Lymphocytes / Q000276:immunology*
D015703:Antigens, CD; D015704:CD4 Antigens; D006023:Glycoproteins; D007136:Immunoglobulins; D007519:Isoantigens; D011948:Receptors, Antigen, T-Cell; D011956:Receptors, Cell Surface; D015375:Receptors, Interleukin-2; C000604059:SLAMF1 protein, human; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1