title: Expression of somatostatin mRNA and peptides in C-cell tumours of the thyroid gland in Han Wistar rats.
abstract: C-cell tumours of the thyroid gland are among the most common spontaneous neoplasms of the laboratory rat. With the exception of calcitonin, little attention has been paid to the secretory peptides of C cells during the development of neoplasia. Of these peptides, somatostatin (SS) is of particular interest because it has been shown to have a direct anti-secretory effect on both thyroid follicular and C cells in vitro. In the present study, in situ hybridization and immunohistochemistry were used to investigate the expression of SS mRNA and SS peptides, in normal C cells and a range of spontaneous proliferative C-cell lesions in the Han Wistar rat. It was confirmed that a small minority of C cells in the normal rat thyroid gland produce and store SS peptides; however, approximately half of all C-cell adenomas and C-cell carcinomas stained positively for SS mRNA and peptides. SS expression was also observed in all metastatic deposits of carcinomas in drainage lymph nodes. From these observations, it appears that C-cell tumours are more likely to develop from SS-expressing stem cells, rather than from non-SS-expressing stem cells. In addition, a lack of differentiation of neoplastic C cells, or reversion to more primitive cell types, could account for increased number of cells expressing SS in C-cell tumours relative to the normal C-cell population. Finally, the mean percentage of cells that stained positively for SS mRNA and peptides appeared to be significantly higher in small C-cell tumours, suggesting that SS may have exerted a growth-controlling influence on these lesions.
authors.affiliation: GlaxoSmithKline Research and Development, Ware, Hertfordshire, UK. pillinga@ukorg.huntingdon.com
authors.forename: Andrew
authors.initials: A
authors.lastname: Pilling
authors.forename: Stewart
authors.initials: S
authors.lastname: Jones
authors.forename: John
authors.initials: J
authors.lastname: Turton
mesh_terms: D000236:Adenoma / Q000378:metabolism / Q000473:pathology; D000818:Animals; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology; D005260:Female; D015870:Gene Expression; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D008297:Male; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000096:biosynthesis* / Q000235:genetics; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D009363:Neoplasm Proteins; D010455:Peptides; D012333:RNA, Messenger; D013004:Somatostatin