Effects of triethylene tetraamine on telomerase activity and proliferation in HeLa cells.
Telomerase, which is required to maintain telomeres, has attracted considerable attention as a target for anticancer therapy. In this study, we investigated the inhibition of HeLa cell telomerase activity and cell cycle progression by triethylene tetraamine (TETA), using a modified telomeric repeat amplification protocol (TRAP) assay, and flow cytometry. TETA inhibited telomerase activity in HeLa cell extracts, with an IC50 of about 7.8 microM. Coupled with this inhibition, TETA also increased the proportion of cells in the G1 phase of the cell cycle in a dose-dependent manner. These findings demonstrate that TETA is a potent inhibitor of telomerase in micromolar concentrations, and inhibits the proliferation of HeLa cells by arresting them in G1.
Yin
Fei
F
State Key Laboratory of Fine Chemicals, Dalian University of Technology, 158 Zhong-shan Road, Dalian 116012, China.
Liu
Jianhui
J
Peng
Xiaojun
X
D002453:Cell Cycle / Q000187:drug effects*; D004789:Enzyme Activation / Q000187:drug effects; D016193:G1 Phase / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D019098:Telomerase / Q000378:metabolism*; D014266:Trientine / Q000633:toxicity*
D019098:Telomerase; D014266:Trientine