Comparison of linear regression and probit analysis for detecting H-reflex threshold in individuals with and without spinal cord injury.
A major challenge to understanding spinal reflex organization in health and disease is identifying sensitive measures of reflex excitability. The purpose of this study was to determine whether linear regression or probit analysis techniques are more sensitive for detecting H-reflex and M-wave threshold and for identifying differences in H-reflex threshold in individuals with and without spinal cord injury (SCI). Soleus H-reflex recruitment curves were generated in 9 individuals with SCI and 20 able-bodied individuals. H-reflex and M-wave threshold was estimated using three different methods, two that used linear regression of H-reflex peak-to-peak amplitude and one that used probit analysis of quantal H-reflexes. Results indicate that in both groups all three techniques were equally sensitive for detecting H-reflex but not M-wave threshold. When H-reflex threshold was normalized to M-wave threshold, different techniques provided different estimates of H-reflex threshold. However, between-group differences (SCI vs. able-body) in H-reflex and M-wave threshold were not affected by the measurement techniques that were compared in this study. We conclude that these techniques provide equally sensitive estimates of H-reflex but not M-wave threshold in persons with and without SCI. Hence, caution should be used when interpreting normalized and non-normalized values of H-reflex threshold.
Schindler-Ivens
S M
SM
The University of Iowa, Graduate Program in Physical Therapy and Rehabilitation Science, Iowa City, IA, USA. s-schindler-ivens@northwestern.edu
Shields
R K
RK
D000328:Adult; D004576:Electromyography / Q000706:statistics & numerical data*; D005260:Female; D006181:H-Reflex / Q000502:physiology*; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D015233:Models, Statistical; D009046:Motor Neurons / Q000502:physiology; D018482:Muscle, Skeletal / Q000294:innervation; D011999:Recruitment, Neurophysiological / Q000502:physiology*; D012016:Reference Values; D012021:Reflex, Abnormal / Q000502:physiology*; D012684:Sensory Thresholds / Q000502:physiology; D012815:Signal Processing, Computer-Assisted; D013119:Spinal Cord Injuries / Q000503:physiopathology*