4Pi-microscopy of the Golgi apparatus in live mammalian cells.
We report the applicability of 4Pi-microscopy to live mammalian cells. Controlled interference of the counterpropagating wavefronts is possible despite the slight variations in cellular refractive index. Superresolved 3D-fluorescence imaging is exemplified with the first representation of the Golgi apparatus in a live cell at approximately 100 nm resolution.
Egner
Alexander
A
Department of NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry, High Resolution Optical Microscopy Group, 37077 Goettingen, Germany.
Verrier
Sophie
S
Goroshkov
Alexander
A
Söling
Hans-Dieter
HD
Hell
Stefan W
SW
D000818:Animals; D002522:Chlorocebus aethiops; D006056:Golgi Apparatus / Q000201:enzymology / Q000648:ultrastructure*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D015238:Sulfotransferases / Q000378:metabolism; D014709:Vero Cells; D006022:beta-N-Acetylglucosaminylglycopeptide beta-1,4-Galactosyltransferase / Q000378:metabolism
D049452:Green Fluorescent Proteins; D006022:beta-N-Acetylglucosaminylglycopeptide beta-1,4-Galactosyltransferase; D015238:Sulfotransferases