title: Efficacy of different commercial and new inactivated vaccines against ovine enzootic abortion.
abstract: The protective efficacy of two inactivated commercial (A, B) and two new inactivated vaccines (M7, QS) against ovine enzootic abortion was determined in two separate experiments in sheep. Vaccine A contained chlamydiae propagated in chicken embryos, adjuvated with Marcol 82, and vaccine B contained chlamydiae cultured in cell monolayers, adjuvated with aluminium hydroxide. For the preparation of the experimental vaccines, Chlamydophila abortus AB7 strain was cultured in McCoy cells and adjuvated with QS-21 (QS) or Montanide ISA 773 (M7). The ewes were vaccinated twice subcutaneously and challenged at 90 days of gestation. Protection was evaluated by clinical, bacteriological and serological examinations, and compared to two control groups: one of infected but not vaccinated ewes, and another of vaccinated but not infected ewes. The experimental vaccines induced considerably better protection than the two commercial ones. The new vaccine M7 especially showed no abortions, a good antibody response, the highest newborn lamb weights and the lowest level of C. abortus shedding at lambing.
authors.affiliation: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, Unidad de Microbiología e Inmunología, Campus de Vegazana, s/n, 24007 León, Spain.
authors.forename: J N
authors.initials: JN
authors.lastname: García de la Fuente
authors.forename: C B
authors.initials: CB
authors.lastname: Gutiérrez-Martín
authors.forename: N
authors.initials: N
authors.lastname: Ortega
authors.forename: E F
authors.initials: EF
authors.lastname: Rodríguez-Ferri
authors.forename: M L
authors.initials: ML
authors.lastname: del Río
authors.forename: O R
authors.initials: OR
authors.lastname: González
authors.forename: J
authors.initials: J
authors.lastname: Salinas
mesh_terms: D000034:Abortion, Veterinary / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D000818:Animals; D000831:Animals, Newborn; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000276:immunology / Q000627:therapeutic use*; D001831:Body Temperature; D001835:Body Weight; D023501:Chlamydophila / Q000276:immunology*; D023521:Chlamydophila Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008297:Male; D011247:Pregnancy; D011897:Random Allocation; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary*; D015164:Vaccines, Inactivated / Q000276:immunology / Q000627:therapeutic use
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D015164:Vaccines, Inactivated