title: A new test for immunological infertility: an ELISA based on prostasomes.
abstract: Antisperm antibodies (ASA) are present in patients with immunological infertility, but the antigens are poorly characterized. Prostasomes adhere to sperm cells and are recognized as antigens for ASA. This investigation aimed to study the prevalence of antiprostasome antibodies in ASA-classified sera. We studied the reactivity of ASA-positive sera from 116 immunoinfertile patients. Ninety-seven per cent (113 of 116) of the patients' sera contained IgG antibodies against seminal prostasomes. Accordingly, prostasomes are one of the major targets for ASA. An enzyme-linked immunosorbent assay based on prostasomes is simpler to perform than ASA tests presently in use. It is also easier to achieve reproducible and standardized results.
authors.affiliation: Department of Medical Cell Biology, Biomedical Center, University of Uppsala, Uppsala, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Carlsson
authors.forename: B O
authors.initials: BO
authors.lastname: Nilsson
authors.forename: G
authors.initials: G
authors.lastname: Ronquist
authors.forename: M
authors.initials: M
authors.lastname: Lundquist
authors.forename: A
authors.initials: A
authors.lastname: Larsson
mesh_terms: D000906:Antibodies / Q000097:blood* / Q000276:immunology; D000941:Antigens / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007158:Immunologic Techniques / Q000592:standards; D007248:Infertility, Male / Q000276:immunology*; D008297:Male; D015388:Organelles / Q000276:immunology*; D011467:Prostate / Q000166:cytology* / Q000378:metabolism; D015203:Reproducibility of Results; D012661:Semen / Q000276:immunology; D013094:Spermatozoa / Q000276:immunology
chemical_list: D000906:Antibodies; D000941:Antigens