Comparative studies on the spermatotoxic effects of dinoseb and its structurally related chemicals.
Three dinitrophenolic compounds, dinoseb (DNBP; 7.5 mg/kg b.w.), 4,6-dinitro-o-cresol (DNOC; 4, 7.5, 15 mg/kg b.w.), and 2,4-dinitrophenol (DNP; 7.5, 15, 30 mg/kg b.w.) were administered orally to sexually matured Jcl:SD male rats for 5 consecutive days. Half of the males in each group were necropsied at 3 (D3) and 14 (D14) days after the last dosing, respectively, and examined for the effects of dinitrophenols on spermato-/spermiogenesis. DNBP (7.5 mg/kg), DNOC (15 mg/kg), and DNP (30 mg/kg) caused 1, 5, and 0 deaths, respectively, as well as a decreased body weights during the treatment. Although examinations on D3 revealed no treatment-related alterations, DNBP and DNOC resulted in reduced sperm motility and increased incidence of tailless sperm in the cauda epididymis on D14. DNP also caused slightly increased incidence of tailless sperm on D14. These results demonstrate that DNBP, DNOC, and DNP manifest similar spermatotoxic effects at or around a lethal dose in rats.
Takahashi
Ken L
KL
Laboratory of Reproductive Toxicology, Toxicology Division I, Institute of Environmental Toxicology, 4321 Uchimoriya-machi, Mitsukaido-shi, Ibaraki, Japan. k.takahashi@iet.or.jp
Hojo
Hitoshi
H
Aoyama
Hiroaki
H
Teramoto
Shoji
S
D019297:2,4-Dinitrophenol / Q000031:analogs & derivatives* / Q000633:toxicity*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003408:Cresols / Q000633:toxicity; D004138:Dinitrocresols; D004140:Dinitrophenols / Q000633:toxicity; D004822:Epididymis / Q000166:cytology; D005837:Genitalia, Male / Q000187:drug effects; D006540:Herbicides / Q000633:toxicity*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000648:ultrastructure
D003408:Cresols; D004138:Dinitrocresols; D004140:Dinitrophenols; D006540:Herbicides; C024132:4,6-dinitro-o-cresol; D019297:2,4-Dinitrophenol; C018527:dinoseb