Effects of nuclear transfer procedures on ES cell cloning efficiency in the mouse.
Enucleated oocytes receiving mouse embryonic stem (ES) cells develop into fertile young. The developmental potential to young is low, however, and the rate of postnatal death is high. We examined the effect of various nuclear transfer procedures on the in vitro and in vivo developmental potential of nuclear-transferred oocytes. The potential of oocytes receiving ES cells at M phase to develop into blastocysts after fusion by Sendai virus was high compared with that after direct injection (67% vs. 30%). The developmental potential of oocytes receiving ES cells at the M phase is higher than that of oocytes receiving ES cells at the G(1) phase (30-67% vs. 2-5%). Developmental ability to live young was low in all groups (0-4%). Different activation protocols affected the potential to develop into blastocysts to a different extent (27-62%), but did not affect the potential to develop into live young (0-3%). The present study demonstrated that the various conditions examined did not affect the potential of nuclear-transferred oocytes receiving ES cells to develop into live young or the incidence of postnatal death.
Yabuuchi
Akiko
A
Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, Japan.
Yasuda
Yoshiko
Y
Kato
Yoko
Y
Tsunoda
Yukio
Y
D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism*; D019976:Cloning, Organism / Q000379:methods*; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000166:cytology*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008938:Mitosis; D009865:Oocytes / Q000378:metabolism; D029082:Sendai virus / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology*; D013997:Time Factors