Glycoprotein profiling by electrospray mass spectrometry.
This work compares several different methods of site-specific analysis of glycoproteins using electrospray mass spectrometry. The glycoprotein, oLHalpha (ovine luteinizing hormone, alpha-subunit) was chosen as an appropriate example protein for these studies because of its biological relevance and extreme microheterogeneity. More than 20 unique glycoforms were detected for this glycoprotein at the Asn(56) site of oLHalpha. The carbohydrates present at this site affect receptor binding affinity, so understanding the great variety in the composition of these carbohydrates is important in studying ligand binding interactions. MS data was acquired on a quadrupole ion trap, a triple quadrupole, and a quadrupole time of flight mass spectrometer, and carbohydrate composition at the Asn(56) site of oLHalpha was determined using these instruments. Additionally, neutral loss and precursor ion scanning modes were also used to identify the glycoforms present, and these techniques were compared to the standard MS data. Of the three instruments compared in the study, the qTOF mass spectrometer achieved the lowest sample consumption, but all three instruments were useful in profiling the glycopeptide composition.
Jiang
Hui
H
Department of Chemistry, University of Kansas, Lawrence, Kansas 66045, USA.
Desaire
Heather
H
Butnev
Vladimir Y
VY
Bousfield
George R
GR
D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification; D009005:Monosaccharides / Q000032:analysis / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D006023:Glycoproteins; D009005:Monosaccharides; D010446:Peptide Fragments