No detectable transfer of dietary lactoferrin or its functional fragments to portal blood in healthy adult rats.
We investigated the transfer of dietary bovine lactoferrin (LF) and its functional lactoferricin (LFcin) B-containing fragments to the portal blood of healthy adult rats by using several techniques. After a single administration of (125)I-labeled LF, radioactive bands were detected in autoradioluminograms of the portal blood, but similar bands were also observed after the administration of [(125)I]NaI. Although ovalbumin was detected by ELISA at 3-18 ng/ml in the portal blood plasma after an overnight administration, no LF was detected (< or =1.5 ng/ml). The antibody-captured ovalbumin fragments, but not the LF fragments, were detected in the plasma by surface-enhanced laser desorption/ionization affinity mass spectrometry (SELDI affinity MS). We finally attempted to detect the LFcin B-containing fragments by SELDI affinity MS with on-chip LFcin B-conversion, but could not detect them (< or =1 ng/ml) in the portal blood after the LF ingestion. The level of LF or its functional fragments transferred to the portal blood was therefore extremely low, if any.
Wakabayashi
Hiroyuki
H
Nutritional Science Laboratory, Morinaga Milk Industry Co., Ltd., Zama, Kanagawa, Japan. h_wakaby@morinagamilk.co.jp
Kuwata
Hidefumi
H
Yamauchi
Koji
K
Teraguchi
Susumu
S
Tamura
Yoshitaka
Y
D000375:Aging / Q000502:physiology; D000821:Animal Feed; D000818:Animals; D000906:Antibodies / Q000276:immunology; D004032:Diet; D004797:Enzyme-Linked Immunosorbent Assay; D006262:Health; D007781:Lactoferrin / Q000008:administration & dosage* / Q000031:analogs & derivatives* / Q000097:blood* / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D010047:Ovalbumin / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry / Q000276:immunology; D010434:Pepsin A / Q000378:metabolism; D010446:Peptide Fragments / Q000097:blood* / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D051381:Rats
D000906:Antibodies; D010446:Peptide Fragments; C082171:lactoferricin B; D010047:Ovalbumin; D007781:Lactoferrin; D010434:Pepsin A