Molecular evidence of tumour cell removal from salvaged blood after irradiation and leucocyte depletion.
Intra-operative autologous blood recovery offers many advantages. However, blood salvage during cancer surgery is of limited use due to the potential presence of circulating tumour cells. It was the aim of this study to show that intra-operative salvage blood can be freed of cells and cellular DNA after leucoreduction by filtration and irradiation of washed blood. Known amounts of tissue culture derived from carcinoma, melanoma and osteosarcoma were added to whole blood bags. This mixture was then submitted to washing, leucoreduction and irradiation. Samples were studied stepwise in relation to the integrity and size of DNA by the polymerase chain reaction (PCR). After filtration and irradiation, PCR targeting the beta-globin gene (268 bp amplicon) was negative. Our results were corroborated by studying plasma samples added with tumoural cells. Using PCR methodology, we showed the absence of DNA from cells in experimentally contaminated blood and plasma bags after filtration and irradiation. This experimental study is an effort to ensure the safety of intra-operative autologous transfusion.
Poli
M C C
MC
Transfusion Medicine Department, Centro de Tratamento e Pesquisa Hospital do Câncer, Rua Professor Antonio Prudente, 211-CEP:01509-010, São Paulo, Brazil. mccpoli@hcancer.org.br
Villa
L L
LL
Colella
R
R
Deheinzelin
D
D
D001773:Blood Cells / Q000473:pathology / Q000528:radiation effects*; D001804:Blood Transfusion, Autologous / Q000379:methods / Q000592:standards*; D004273:DNA, Neoplasm / Q000032:analysis; D005374:Filtration; D005914:Globins / Q000235:genetics; D006801:Humans; D047589:Leukocyte Reduction Procedures; D008954:Models, Biological; D009369:Neoplasms / Q000473:pathology / Q000628:therapy*; D009360:Neoplastic Cells, Circulating / Q000473:pathology* / Q000528:radiation effects; D016133:Polymerase Chain Reaction
D004273:DNA, Neoplasm; D005914:Globins