title: Restriction of bovine herpesvirus 1 (BHV-1) growth in non-permissive cells beyond the expression of immediate early genes.
abstract: Mouse BALB/3T3-A31-1-1 (A31) cells are non-permissive to bovine herpes virus-1 (BHV-1) but permissive to pseudorabies virus (PrV). The promoter activity of the immediate early gene of BHV-1 (BICP4) was very weak when compared with that of PrV in A31 cells. Infectious BHV-1 genomic DNA co-transfected into A31 cells with plasmids expressing BICP4 and BICP0 by a strong promoter failed to yield any progeny virus. Growth of BHV-1 in non-permissible A31 cells is restricted in many phases of the growth. The fact that expression of BICP4 and/or BICP0 in A31 cells does not improve the yield of progeny virus from infectious BHV-1 genomic DNA suggests that some more growth restrictions exist beyond the expression of BHV-1 immediate early proteins.
authors.affiliation: Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
authors.forename: Orkash
authors.initials: O
authors.lastname: Hushur
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Takashima
authors.forename: Yasunobu
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Haruki
authors.initials: H
authors.lastname: Otsuka
mesh_terms: D000818:Animals; D041702:BALB 3T3 Cells; D015153:Blotting, Western; D017931:DNA Primers; D015967:Gene Expression Regulation, Viral; D007242:Herpesvirus 1, Bovine / Q000235:genetics / Q000254:growth & development*; D011558:Herpesvirus 1, Suid / Q000254:growth & development*; D017874:Immediate-Early Proteins / Q000235:genetics; D051379:Mice; D008856:Microscopy, Fluorescence; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D015534:Trans-Activators; D014162:Transfection; D044767:Ubiquitin-Protein Ligases; D014759:Viral Envelope Proteins / Q000235:genetics*; D014764:Viral Proteins
chemical_list: D017931:DNA Primers; D017874:Immediate-Early Proteins; D015534:Trans-Activators; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C083786:immediate early protein BICP4, bovine herpesvirus 1; D044767:Ubiquitin-Protein Ligases; C051804:Vmw110 protein, Human herpesvirus 1; C086729:bICP0 protein, Bovine herpesvirus 1