[Selection of scaffolds of rat hepatocytes in three-dimension culture under simulated microgravity].
In order to investigate the biocompatibility, degradation and inflammatory reactions of poly benzyl glutamate (PBLG), poly lactic-co-glycolic acid (PLGA) and fibrin gel used as the scaffolds co-cultured with the hepatocytes of rats under simulated microgravity conditions in rotating wall vessel bioreactor (RWVB). PLGA is low biocompatibility, PBLG is medium biocompatibility with rat hepatocytes. Fibrin gel is high biocompatibility, no toxic degradation and no inflammatory reactions. Fibrin gel can be used as three dimensional scaffolds of rat hepatocytes under simulated microgravity.
Zhang
Shou Quan
SQ
College of Animal Science, South China Agricultural University, Guangzhou 510642.
Gao
Shu Jing
SJ
Jiang
Qing Yan
QY
Lao
Quan Lin
QL
Feng
Ding Yuan
DY
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D019149:Bioreactors; D018929:Cell Culture Techniques; D005337:Fibrin / Q000737:chemistry*; D006016:Glycolates / Q000737:chemistry; D022781:Hepatocytes / Q000166:cytology*; D019344:Lactic Acid; D008422:Materials Testing; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017207:Rats, Sprague-Dawley; D018474:Weightlessness Simulation
D001672:Biocompatible Materials; D006016:Glycolates; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; C003172:poly-gamma-benzyl-L-glutamate; D011099:Polyglutamic Acid; D011100:Polyglycolic Acid; D019344:Lactic Acid; D005337:Fibrin