title: A novel encoded particle technology that enables simultaneous interrogation of multiple cell types.
abstract: The authors have developed a cellular analysis platform, based on encoded microcarriers, that enables the multiplexed analysis of a diverse range of cellular assays. At the core of this technology are classes of microcarriers that have unique, identifiable codes that are deciphered using CCD-based imaging and subsequent image analysis. The platform is compatible with a wide variety of cellular imaging-based assays, including calcium flux, reporter gene activation, cytotoxicity, and proliferation. In addition, the platform is compatible with both colorimetric and fluorescent readouts. Notably, this technology has the unique ability to multiplex different cell lines in a single microplate well, enabling scientists to perform assays and data analysis in novel ways.
authors.affiliation: Vitra Bioscience, Inc., Mountain View, CA 94043, USA. obeske@vitrabio.com
authors.forename: Oren
authors.initials: O
authors.lastname: Beske
authors.forename: Jinjiao
authors.initials: J
authors.lastname: Guo
authors.forename: Jianren
authors.initials: J
authors.lastname: Li
authors.forename: Daniel
authors.initials: D
authors.lastname: Bassoni
authors.forename: Kimberly
authors.initials: K
authors.lastname: Bland
authors.forename: Holly
authors.initials: H
authors.lastname: Marciniak
authors.forename: Mike
authors.initials: M
authors.lastname: Zarowitz
authors.forename: Vladimir
authors.initials: V
authors.lastname: Temov
authors.forename: Ilya
authors.initials: I
authors.lastname: Ravkin
authors.forename: Simon
authors.initials: S
authors.lastname: Goldbard
mesh_terms: D000818:Animals; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D002217:Carbachol / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D018914:Cells, Immobilized; D006224:Cricetinae; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007609:Kallidin / Q000494:pharmacology; D008853:Microscopy / Q000295:instrumentation / Q000379:methods; D008967:Molecular Biology / Q000379:methods*; D010316:Particle Size; D043782:Receptor, Bradykinin B2 / Q000032:analysis / Q000187:drug effects / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000032:analysis / Q000187:drug effects / Q000235:genetics; D011976:Receptors, Muscarinic / Q000032:analysis / Q000187:drug effects / Q000235:genetics; D015398:Signal Transduction; D018675:Toxicity Tests
chemical_list: D043782:Receptor, Bradykinin B2; D043562:Receptors, G-Protein-Coupled; D011976:Receptors, Muscarinic; D007609:Kallidin; D002217:Carbachol; D002118:Calcium