[Effect of bovine serum albumin and restriction enzyme on quality of DNA profile of tissues extracted from paraffin blocks and histological slides].
This paper reports the possibility of DNA profiling obtained from fixed and paraffin-embedded tissues or histological slides. The influence of using bovine serum albumin and restriction enzyme Hinfl on quality of DNA was analysed. The usability of those chemicals was estimated by examination of amplification results locus VWA, and multiplex PCR with the use commercial kit AmpFlSTR Identifiler (including 15 loci + amelogenine locus) and AmpFlSTR SEfiler kit (including 11 loci + amelogenine locus) Applied Biosystems. PCR products of VWA locus were separated by electrophoresis on polyacrylamide gels and visualized by silver staining. The products of loci of AmpFlSTR Identifiler and AmpFlSTR SEfiler were analyzed by capillary electrophoresis on an ABI Prism 310 sequencer. Results achieved with and without using bovine serum albumin and restriction enzyme Hinfl were compared and positive influence of those substances on DNA quality was demonstrated. Obtained results of purification DNA with the use of restriction enzyme were verified in paternity testing case.
Piasecka-Pazik
Dorota
D
Katedry i Zakładu Medycyny Sadowej AM w Gdańsku.
Szczerkowska
Zofia
Z
D000818:Animals; D001344:Autopsy; D001801:Blood Stains; D002417:Cattle; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000379:methods*; D004262:DNA Restriction Enzymes; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic / Q000235:genetics; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine; D020080:Tandem Repeat Sequences / Q000235:genetics
D012710:Serum Albumin, Bovine; D004247:DNA; D004262:DNA Restriction Enzymes