title: Genomic aberrations in carcinomas of the uterine corpus.
abstract: Endometrial carcinoma, the most common invasive neoplasm of the female genital tract, occurs either in a hormone-related, less virulent form (type I) or in a hormone-independent, more aggressive form (type II). Another cancer of the uterine corpus is carcinosarcoma, a biphasic or mixed epithelial-mesenchymal tumor, now classified as metaplastic carcinoma. We examined by karyotyping and comparative genomic hybridization a consecutive series of 67 endometrial carcinomas and 15 carcinosarcomas and compared the cytogenetic features of the different carcinoma subtypes. All three subtypes of uterine carcinoma had in common a nonrandom gain of material from 1q and 8q but differed from one another in other respects. Endometrial carcinomas of type I mostly presented gains from chromosome arms 1q and 8q and losses from Xp, 9p, 9q, 17p, 19p, and 19q, whereas endometrial carcinomas of type II showed a more complex imbalance picture, with gains from chromosome arms 1q, 2p, 3q, 5p, 6p, 7p, 8q, 10q, and 20q and losses from Xq, 5q, and 17p. The carcinosarcomas mostly showed gains of or from 1q, 5p, 8q, and 12q but losses from 9q, that is, they were much more similar to endometrial carcinomas in their pattern of acquired genomic changes than to sarcomas of the uterine corpus. It was also possible to identify different copy number changes among the different grades of type I carcinomas, between serous papillary and clear-cell carcinomas of type II, as well as between homologous and heterologous carcinosarcomas. Specifically, type I adenocarcinomas that were highly differentiated mostly showed gains from 1q and 10p; those that were moderately differentiated showed gains from 1q, 7p, 7q, and 10q as well as losses from Xp, 9p, 9q, 17p, 19p, and 19q; whereas those poorly differentiated showed gains from 1q, 2p, 2q, 3q, 6p, 8q, and 20q but losses from Xp, Xq, 5q, 9p, 9q, 17p, and 17q. The serous papillary carcinomas showed gains from 1q, 2p, 2q, 3q, 5p, 6p, 6q, 7p, 8q, 18q, 20p, and 20q but losses from 17p, whereas the clear-cell carcinomas showed gains from 3q, 7p, 8q, 10q, 16p, and 20q but losses from 6q. Finally, the homologous carcinosarcomas presented gains from 1p, 1q, 8q, 12q, and 17q as well as losses from 9q and 13q, whereas the heterologous tumors showed gains from 1q, 8p, and 8q. The reproducibility of the observed correlations between karyotypic aberration patterns and histological differentiation was underscored by the fact that those carcinosarcomas whose epithelial component resembled type I endometrial carcinomas also exhibiting a type I aberration profile, whereas carcinosarcomas with a type II carcinoma differentiation had karyotypic abnormalities similar to those of type II endometrial carcinomas.
authors.affiliation: Department of Cancer Genetics, The Norwegian Radium Hospital, Oslo, Norway.
authors.forename: Francesca
authors.initials: F
authors.lastname: Micci
authors.forename: Manuel R
authors.initials: MR
authors.lastname: Teixeira
authors.forename: Lisbeth
authors.initials: L
authors.lastname: Haugom
authors.forename: Gunnar
authors.initials: G
authors.lastname: Kristensen
authors.forename: Vera M
authors.initials: VM
authors.lastname: Abeler
authors.forename: Sverre
authors.initials: S
authors.lastname: Heim
mesh_terms: D002277:Carcinoma / Q000145:classification / Q000235:genetics*; D002296:Carcinosarcoma / Q000145:classification / Q000235:genetics; D002869:Chromosome Aberrations / Q000145:classification*; D002871:Chromosome Banding / Q000379:methods; D002872:Chromosome Deletion; D020732:Cytogenetic Analysis; D016889:Endometrial Neoplasms / Q000145:classification / Q000235:genetics; D005260:Female; D005784:Gene Amplification / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D007621:Karyotyping; D008679:Metaplasia / Q000145:classification / Q000151:congenital / Q000235:genetics; D009693:Nucleic Acid Hybridization / Q000379:methods; D014594:Uterine Neoplasms / Q000145:classification / Q000235:genetics*; D014599:Uterus / Q000473:pathology