Saccharomyces cerevisiae SSD1-V confers longevity by a Sir2p-independent mechanism.
The SSD1 gene of Saccharomyces cerevisiae is a polymorphic locus that affects diverse cellular processes including cell integrity, cell cycle progression, and growth at high temperature. We show here that the SSD1-V allele is necessary for cells to achieve extremely long life span. Furthermore, addition of SSD1-V to cells can increase longevity independently of SIR2, although SIR2 is necessary for SSD1-V cells to attain maximal life span. Past studies of yeast aging have been performed in short-lived ssd1-d strain backgrounds. We propose that SSD1-V defines a previously undescribed pathway affecting cellular longevity and suggest that future studies on longevity-promoting genes should be carried out in long-lived SSD1-V strains.
Kaeberlein
Matt
M
Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA. kaeber@u.washington.edu
Andalis
Alex A
AA
Liszt
Gregory B
GB
Fink
Gerald R
GR
Guarente
Leonard
L
D000483:Alleles; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics; D006655:Histone Deacetylases / Q000378:metabolism*; D008136:Longevity / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D009994:Osmolar Concentration; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae / Q000378:metabolism*; D056565:Sirtuin 2; D037761:Sirtuins / Q000378:metabolism*; D013696:Temperature; D014170:Transformation, Genetic
D029701:Saccharomyces cerevisiae Proteins; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae; C485219:Ssd1 protein, S cerevisiae; C087778:SIR2 protein, S cerevisiae; D056565:Sirtuin 2; D037761:Sirtuins; D006655:Histone Deacetylases