Ion transport mechanisms in the mesonephric collecting duct system of the toad Bufo bufo: microelectrode recordings from isolated and perfused tubules.
It is not clear how and whether terrestrial amphibians handle NaCl transport in the distal nephron. Therefore, we studied ion transport in isolated perfused collecting tubules and ducts from toad, Bufo bufo, by means of microelectrodes. No qualitative difference in basolateral cell membrane potential (Vbl) was observed between tubules and ducts in response to ion substitutions, inhibitor and agonist applications. Cl- substitution experiments indicated a small Cl- conductance in the basolateral membrane. The apical membrane did not have a significant Cl- conductance. Luminal [Na+] steps and amiloride application showed a small apical Na+ conductance. Arginine vasotocin depolarized Vbl. The small apical Na+ conductance indicates that the collecting duct system contributes little to NaCl reabsorption when compared to aquatic amphibians. In contrast, Vbl rapidly depolarized upon lowering of [Na+] in the bath, demonstrating the presence of a Na+-coupled anion transporter. [HCO3-] steps revealed that this transporter is not a Na+-HCO3- cotransporter. Together, our results indicate that a major task of the collecting duct system in B. bufo is not conductive NaCl transport but rather K+ secretion, as shown by our previous studies. Moreover, our results indicate the presence of a novel basolateral Na+-coupled anion transporter, the identity of which remains to be elucidated.
Møbjerg
Nadja
N
Zoophysiological Laboratory, August Krogh Institute, University of Copenhagen, Universitetsparken 13, DK-2100 Copenhagen Ø, Denmark. nmobjerg@aki.ku.dk
Larsen
Erik Hviid
EH
Novak
Ivana
I
D000818:Animals; D002023:Bufo bufo / Q000196:embryology / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D004594:Electrophysiology / Q000379:methods; D004625:Embryo, Nonmammalian; D066298:In Vitro Techniques; D017136:Ion Transport / Q000187:drug effects / Q000502:physiology*; D007545:Isoproterenol / Q000494:pharmacology; D007685:Kidney Tubules, Collecting / Q000196:embryology / Q000378:metabolism*; D008650:Mesonephros / Q000378:metabolism*; D008839:Microelectrodes; D010477:Perfusion; D012964:Sodium / Q000378:metabolism; D014668:Vasotocin / Q000494:pharmacology
D002712:Chlorides; D012964:Sodium; D007545:Isoproterenol; D014668:Vasotocin