title: Attachment and infection to MA104 cells of avian rotaviruses require the presence of sialic acid on the cell surface.
abstract: To determine the characters of receptors on target cells for avian rotaviruses, the receptors on MA104 cells for the pigeon rotavirus PO-13, the turkey rotaviruses Ty-1 and Ty-3, and the chicken rotavirus Ch-1 were analyzed. Pretreatment of MA104 cells with neuraminidase greatly reduced the infection by all of the four avian rotavirus strains. Binding of the cell-attachment protein, purified VP8 expressed in bacteria, of strain PO-13 to MA104 cells was also inhibited by pretreatment of cells with neuraminidase. These findings suggest that avian rotaviruses primarily utilize sialic acid-containing molecules as receptors on MA 104 cells.
authors.affiliation: Laboratory of Zoonotic Diseases, Faculty of Agriculture, Gifu University, Yanagido, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Sugiyama
authors.forename: Kazuo
authors.initials: K
authors.lastname: Goto
authors.forename: Hiroko
authors.initials: H
authors.lastname: Uemukai
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Mori
authors.forename: Naoto
authors.initials: N
authors.lastname: Ito
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Minamoto
mesh_terms: D000818:Animals; D001717:Birds / Q000821:virology*; D015153:Blotting, Western; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005982:Glutathione Transferase / Q000378:metabolism; D009439:Neuraminidase / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012401:Rotavirus / Q000378:metabolism*
chemical_list: D036022:Capsid Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D011956:Receptors, Cell Surface; C092488:sialic acid receptor; D005982:Glutathione Transferase; D009439:Neuraminidase