title: NY-ESO-1 expression and its serum immunoreactivity in esophageal cancer.
abstract: OBJECTIVE
  NY-ESO-1, a member of the cancer/testis antigen (CTA) family, elicits humoral and cellular immune responses in patients with advanced cancer. Unresectable or metastatic esophageal carcinoma patients do not benefit from the present multimodality treatment regimens in terms of survival. The objectives of this study were to analyze the antibody response to NY-ESO-1 antigen in patients with esophageal cancer and to determine the potential of NY-ESO-1 for use in tumor-specific immunotherapy.
  
  
  METHODS
  Serum from 69 patients with esophageal cancer was investigated for antibody production against NY-ESO-1 by Western blot analysis. Also analyzed by immunohistochemistry were 56 tissue samples from these patients for NY-ESO-1 protein expression.
  
  
  RESULTS
  NY-ESO-1 protein expression was found in 18 of 56 (32%) esophageal carcinomas. Serum immunoreactivity specific for NY-ESO-1 was found in 9 patients (13%) of whom 8 were in the advanced stage (stages III and IV). There was no relationship between clinicopathologic features and serum immunoreactivity for NY-ESO-1. NY-ESO-1 protein expression was detected in three of five antibody-positive patients whose tissue was available for analysis. Survival analysis showed no significant difference between antibody-positive and antibody-negative patient groups.
  
  
  CONCLUSIONS
  A humoral immune response to NY-ESO-1 antigen was established in patients with advanced esophageal cancer. NY-ESO-1 is a good candidate for vaccine-based immunotherapy for advanced esophageal carcinoma.
authors.affiliation: Division of Digestive and General Surgery, Niigata University Graduate School of Medical and Dental Sciences, 951-8510 Niigata, Japan.
authors.forename: Argun
authors.initials: A
authors.lastname: Akcakanat
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Kanda
authors.forename: Yu
authors.initials: Y
authors.lastname: Koyama
authors.forename: Michitoshi
authors.initials: M
authors.lastname: Watanabe
authors.forename: Eiji
authors.initials: E
authors.lastname: Kimura
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Shintarou
authors.initials: S
authors.lastname: Komukai
authors.forename: Satoru
authors.initials: S
authors.lastname: Nakagawa
authors.forename: Shoji
authors.initials: S
authors.lastname: Odani
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Fujii
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Hatakeyama
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000917:Antibody Formation; D000951:Antigens, Neoplasm / Q000096:biosynthesis* / Q000276:immunology*; D015153:Blotting, Western; D019496:Cancer Vaccines / Q000276:immunology; D004938:Esophageal Neoplasms / Q000276:immunology* / Q000628:therapy; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D008875:Middle Aged; D013737:Testis
chemical_list: D000951:Antigens, Neoplasm; C104948:CTAG1B protein, human; D019496:Cancer Vaccines; D008565:Membrane Proteins