Activity of cholinesterases in human whole blood measured with acetylthiocholine as substrate and ethopropazine as selective inhibitor of plasma butyrylcholinesterase.
A procedure is suggested for measuring acetylcholinesterase and butyrylcholinesterase activities in human whole blood using acetylthiocholine as a substrate and ethopropazine as a selective inhibitor of butyrylcholinesterase. The procedure is suitable for screening cholinesterase activities in routine and/or field tests.
Reiner
Elsa
E
Institute for Medical Research and Occupational Health, Zagreb, Croatia. ereiner@imi.hr
Bosak
Anita
A
Simeon-Rudolf
Vera
V
D000109:Acetylcholine; D000110:Acetylcholinesterase / Q000097:blood*; D002091:Butyrylcholinesterase / Q000097:blood*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D004796:Clinical Enzyme Tests / Q000379:methods*; D006801:Humans; D010640:Phenothiazines; D013053:Spectrophotometry / Q000379:methods; D013379:Substrate Specificity
D002800:Cholinesterase Inhibitors; D010640:Phenothiazines; C084820:profenamine; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D000109:Acetylcholine