Starter unit choice determines the production of two tetraene macrolides, rimocidin and CE-108, in Streptomyces diastaticus var. 108.
Streptomyces diastaticus var. 108, a newly isolated strain, produces two closely related tetraene macrolides (rimocidin and CE-108) as well as oxytetracycline. A region of 19,065 base pairs of DNA from the S. diastaticus var. 108 genome was isolated, sequenced, and characterized. Ten complete genes and one truncated ORF were located. Disruption of these genes proved that this genomic region is part of the biosynthetic cluster for the two tetraenes. The choice of starter units by the loading module and the in vivo availability of the starter metabolites are crucial for the final ratio of the two macrolides. A second type I PKS, unrelated to tetraene biosynthesis, was also identified; disruption of these genes suggests that they would code for enzymes involved in the biosynthesis of a polyketide that might compete metabolically with rimocidin production.
Seco
Elena M
EM
Centro Nacional de Biotecnología, Campus de la UAM, 28049 Cantoblanco, Madrid, Spain.
Pérez-Zúñiga
Francisco J
FJ
Rolón
Miriam S
MS
Malpartida
Francisco
F
D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D018942:Macrolides; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009005:Monosaccharides / Q000096:biosynthesis*; D005810:Multigene Family / Q000235:genetics; D015345:Oligonucleotide Probes / Q000235:genetics / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics; D011090:Polyenes / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics
C485858:CE-108; D018942:Macrolides; D009005:Monosaccharides; D015345:Oligonucleotide Probes; D011090:Polyenes; C003273:rimocidin