Frequent detection of viral nucleic acids in heart valve tissue.
Due to a paucity of published data concerning the prevalence of viral nucleic acid in homografts, we analyzed tissue from 30 donor hearts for the presence of viral genome sequences of enteroviruses, adenoviruses, human cytomegalovirus, and influenza virus using different PCR techniques. Viral DNA was amplified in 64 and 52% of the subvalvular myocardial tissue and non-coronary valve samples, respectively. These findings, compared with clinical history and histologic and serologic analysis, demonstrate the importance of viral safety measures in heart valve banking.
Donoso Mantke
Oliver
O
Centre for Biological Safety, Robert Koch-Institut, 13353 Berlin, Germany. donosoo@rki.de
Meyer
Rudolf
R
Prösch
Susanna
S
Niedrig
Matthias
M
D000293:Adolescent; D000328:Adult; D001705:Bioprosthesis / Q000009:adverse effects / Q000821:virology*; D002648:Child; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D006350:Heart Valve Prosthesis / Q000009:adverse effects / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012307:Risk Factors; D012449:Safety; D014019:Tissue Donors; D014184:Transplantation, Homologous; D014777:Virus Diseases / Q000635:transmission
D004279:DNA, Viral