Inhibitors of 5-lipoxygenase inhibit expression of intercellular adhesion molecule-1 in human melanoma cells.
OBJECTIVE
To study the effect of 5-lipoxygenase inhibitors on the expression of intercellular adhesion molecule-1 (ICAM-1) in melanoma cells.


METHODS
ICAM-1 protein of human melanoma cell a375 was detected by enzyme-linked immunosorbent, flow cytometry and Western blot analysis. Level of ICAM-1 mRNA in a375 was evaluated by Northern blot analysis. Adhesion of a375 to endothelial cell EC304 was analyzed by isotopic tracing.


RESULTS
5-Lipoxygenase inhibitors nordihydroguaiaretic acid, AA861 and MK886, could suppress the expression of ICAM-1 protein as well as of its mRNA in a375 cells and reduce the adhesion of a375 to EC304.


CONCLUSIONS
5-Lipoxygenase inhibitors can inhibit the expression of ICAM-1 in human melanoma cells and may be valuable for treatment of melanoma metastasis.
Wang
Yin
Y
Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Zhou
Bin
B
Li
Ji
J
Cao
Yong-bing
YB
Chen
Xin-sheng
XS
Cheng
Ming-he
MH
Yin
Ming
M
D016227:Benzoquinones / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000235:genetics; D016859:Lipoxygenase Inhibitors; D009637:Masoprocol / Q000494:pharmacology*; D008545:Melanoma / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D014407:Tumor Cells, Cultured; D014471:Umbilical Veins / Q000166:cytology; D002722:Chlorobenzenes
D016227:Benzoquinones; D007211:Indoles; D018799:Intercellular Adhesion Molecule-1; D016859:Lipoxygenase Inhibitors; D009637:Masoprocol; D012333:RNA, Messenger; C060893:MK-886; D002722:Chlorobenzenes; C036837:2,3,5-trimethyl-6-(12-hydroxy-5,10-dodecadiynyl)-1,4-benzoquinone