title: Crystal structure of the YDR533c S. cerevisiae protein, a class II member of the Hsp31 family.
abstract: The ORF YDR533c from Saccharomyces cerevisiae codes for a 25.5 kDa protein of unknown biochemical function. Transcriptome analysis of yeast has shown that this gene is activated in response to various stress conditions together with proteins belonging to the heat shock family. In order to clarify its biochemical function, we determined the crystal structure of YDR533c to 1.85 A resolution by the single anomalous diffraction method. The protein possesses an alpha/beta hydrolase fold and a putative Cys-His-Glu catalytic triad common to a large enzyme family containing proteases, amidotransferases, lipases, and esterases. The protein has strong structural resemblance with the E. coli Hsp31 protein and the intracellular protease I from Pyrococcus horikoshii, which are considered class I and class III members of the Hsp31 family, respectively. Detailed structural analysis strongly suggests that the YDR533c protein crystal structure is the first one of a class II member of the Hsp31 family.
authors.affiliation: Laboratoire d'Enzymologie et Biochimie Structurales, CNRS-UPR 9063, Bâtiment 34, 1 Avenue de la Terrasse, 91198 Gif sur Yvette, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Graille
authors.forename: Sophie
authors.initials: S
authors.lastname: Quevillon-Cheruel
authors.forename: Nicolas
authors.initials: N
authors.lastname: Leulliot
authors.forename: Cong-Zhao
authors.initials: CZ
authors.lastname: Zhou
authors.forename: Ines
authors.initials: I
authors.lastname: Li de la Sierra Gallay
authors.forename: Lilian
authors.initials: L
authors.lastname: Jacquamet
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Ferrer
authors.forename: Dominique
authors.initials: D
authors.lastname: Liger
authors.forename: Anne
authors.initials: A
authors.lastname: Poupon
authors.forename: Joel
authors.initials: J
authors.lastname: Janin
authors.forename: Herman
authors.initials: H
authors.lastname: van Tilbeurgh
mesh_terms: D000595:Amino Acid Sequence; D020134:Catalytic Domain; D019281:Dimerization; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D006360:Heat-Shock Proteins; D029701:Saccharomyces cerevisiae Proteins; C496117:HSP31 protein, S cerevisiae