title: Differentiation of preosteoblasts is affected by implant surface microtopographies.
abstract: Osteogenesis involves the recruitment of multipotent mesenchymal cells and the progressive differentiation of these cells into osteoblasts. The transcription factor Runx2 regulates osteoblast differentiation and expression of genes necessary for the development of a mineralized phenotype. The purpose of this study was to determine if preosteoblast cell differentiation associated with Runx2 and osteocalcin gene expression was influenced by implant surface microtopography. Human embryonic palatal mesenchymal cells (HEPM cells) were cultured on grooved or roughened cpTi implant discs. Cell phenotypes were evaluated with epifluorescent microscopy. Real-time PCR was used for quantitative analysis of Runx2 and osteocalcin gene expression. HEPM cells mineralized when grown on rough and grooved implant surfaces relative to tissue culture plastic. Real-time PCR showed significant (p < 0.05) increases in Runx2 and osteocalcin gene expression in cells cultured on rough and grooved implant microtopographies. These results suggest that preosteoblast cell differentiation is affected by implant surface microtopographies during osseointegration of dental implants.
authors.affiliation: Dows Institute for Dental Research and the Department of Prosthodontics, N402, University of Iowa College of Dentistry, Iowa City, Iowa 52242.
authors.forename: Galen B
authors.initials: GB
authors.lastname: Schneider
authors.forename: Rebecca
authors.initials: R
authors.lastname: Zaharias
authors.forename: Denise
authors.initials: D
authors.lastname: Seabold
authors.forename: John
authors.initials: J
authors.lastname: Keller
authors.forename: Clark
authors.initials: C
authors.lastname: Stanford
mesh_terms: D000880:Anthraquinones / Q000378:metabolism; D001483:Base Sequence; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D004396:Coloring Agents / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism*; D019736:Prostheses and Implants; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry; D050656:Transcription Factor AP-2; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000880:Anthraquinones; D001672:Biocompatible Materials; D004396:Coloring Agents; D050659:Core Binding Factor Alpha 1 Subunit; D004268:DNA-Binding Proteins; C493709:RUNX2 protein, human; D050656:Transcription Factor AP-2; D014157:Transcription Factors; D015675:Osteocalcin; C004468:Alizarin Red S; D014025:Titanium