Genetic and epigenetic evaluations of citrus calluses recovered from slow-growth culture.
The embryogenic callus of "Red Marsh" grapefruit was stored in vitro by slow growth culture method for one year, and survived with a significant weight increment over that period. The survivers regenerated somatic embryos more easily than the controls. Eight callus lines were used for genetic analyses. Although chromosome number variations were verified by cytological examination both in the controls and the stored samples, the ploidy level remained relatively stable during the storage period. Randomly amplified polymorphic DNA (RAPD) analysis was performed to detect DNA sequence variation. No difference in RAPD pattern was found with the 102 primers used. However, a methylation sensitive amplified polymorphism (MSAP) assay showed DNA methylation changes in the stored samples compared with the controls.
Hao
Yu-Jin
YJ
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P. R. China. yjcxhao@hotmail.com
Wen
Xiao-Peng
XP
Deng
Xiu-Xin
XX
D002957:Citrus / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D046508:Culture Techniques; D019175:DNA Methylation; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D044127:Epigenesis, Genetic; D011003:Ploidies; D019105:Random Amplified Polymorphic DNA Technique
D018744:DNA, Plant