Investigation of recombinant human elastin polypeptides as non-thrombogenic coatings.
We investigated the use of a recombinant human elastin polypeptide as a coating on synthetic materials with a view to determining if these polypeptides could improve the blood compatibility of cardiovascular devices such as vascular conduits and arterial/venous catheters. Platelet adhesion and activation were studied in vitro using three commercially available synthetic materials: polyethylene terephthalate (Mylar), a poly(tetrafluoroethylene/ethylene) copolymer (Tefzel) and a polycarbonate polyurethane (Corethane). Coated with adsorbed polypeptide, all three synthetic materials demonstrated reduced platelet activation and adhesion in platelet rich plasma in vitro. Compared to non-coated controls, there was a significant decrease (p=0.05) in both platelet microparticle release and P-selectin expression for the polypeptide-coated surfaces. Scanning electron microscopy indicated fewer adhering platelets on coated surfaces compared to non-coated controls. In vivo, in a rabbit model, evaluations of polyurethane catheters coated with the polypeptide showed a marked increase in catheter patency and a significant decrease in fibrin accretion and embolism when compared to uncoated controls. This polypeptide shows a strong potential for use as a non-thrombogenic coating for small diameter vascular grafts. In addition, the results of this study indicate that the elastin polypeptide would be a valuable component of a tissue engineered vascular conduit.
Woodhouse
Kimberly A
KA
Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, Ontario, Canada M5S 3E5. kas@chem-eng.utoronto.ca
Klement
Petr
P
Chen
Vivian
V
Gorbet
Maud B
MB
Keeley
Fred W
FW
Stahl
Richard
R
Fromstein
Joanna D
JD
Bellingham
Catherine M
CM
D000327:Adsorption; D000818:Animals; D001792:Blood Platelets / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D001807:Blood Vessel Prosthesis / Q000009:adverse effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004549:Elastin / Q000008:administration & dosage* / Q000737:chemistry* / Q000235:genetics; D005343:Fibrinolytic Agents / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D008297:Male; D008422:Materials Testing; D019007:P-Selectin / Q000378:metabolism*; D010455:Peptides / Q000008:administration & dosage* / Q000737:chemistry* / Q000235:genetics; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology; D020246:Venous Thrombosis / Q000209:etiology / Q000378:metabolism / Q000473:pathology* / Q000517:prevention & control
D020099:Coated Materials, Biocompatible; D005343:Fibrinolytic Agents; D019007:P-Selectin; D010455:Peptides; D011994:Recombinant Proteins; C495470:elastin polypeptide 20-24-24, human; D004549:Elastin