[Suspension culture of protocorm-like bodies from the endangered medicinal plant Dendrobium huoshanenese].
OBJECTIVE
To investigate the characteristics of growth, and water-soluble polysaccharide and total alkaloid accumulation in protocom-like bodies (PLBs) of Dendrobium huoshanenese in liquid culture system.


METHODS
PLBs were suspended in liquid medium and growth kinetics was analyzed. Water-soluble polysaccharide and total alkaloid content in PLBs were determined by colorimetry.


RESULTS
PLBs were induced from stem explants of D. huoshanenese regenerants cultured on MS medium supplemented with NAA or NAA and KT at different concentrations. Basal MS medium was suitable for propagation of PLBs. When PLBs were suspended in liquid medium, mumax was 0.044.d-1, t(d) was 15.8 d and the optimum growth time was 4 weeks. Water-soluble polysaccharide and total alkaloid contents in PLBs were 3.75% and 0.0261%, respectively.


CONCLUSIONS
PLBs in liquid culture system show a potential for rapid growth and high metabolite synthesis, which provides possibility for exploiting resources of D. huoshanenese by large scale culture of PLBs.
Luo
Jiang-ping
JP
College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, Anhui, China. jianpinglou@sohu.com
Zha
Xue-qiang
XQ
Jiang
Shao-tong
ST
D000470:Alkaloids / Q000032:analysis; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D031667:Dendrobium / Q000737:chemistry / Q000254:growth & development*; D018547:Plant Stems / Q000254:growth & development; D010946:Plants, Medicinal / Q000737:chemistry / Q000254:growth & development*; D011134:Polysaccharides / Q000032:analysis
D000470:Alkaloids; D003470:Culture Media; D011134:Polysaccharides