Delayed interconversion of D-glucose anomers in D2O.
The anomeric specificity of D-glucose metabolism and functional effects is currently assessed either over about 5 min at 37 degrees C or longer periods at much lower temperatures. In the present study, the half-life for the interconversion of D-glucose anomers at 30 degrees C was found to be increased (P<0.005) from 17.2+/-0.9 min in a mixture of H2O/D2O (9/1, v/v) to 58.6+/-1.9 min in pure D2O. It is proposed that advantage can be taken from this delayed interconversion in D2O in experiments dealing with the anomeric specificity of D-glucose metabolic fate and functional effects. Thus, even over 60-min incubation at 30 degrees C, the integrated mean abundance of alpha- and beta-D-glucose would remain close to 82 and 89%, respectively, of the corresponding initial value (100%).
Malaisse
Willy J
WJ
Laboratory of Experimental Hormonology, Université Libre de Bruxelles, B-1070 Brussels, Belgium. malaisse@ulb.ac.be
Verbruggen
Ingrid
I
Biesemans
Monique
M
Willem
Rudolph
R
D002250:Carbon Radioisotopes; D005947:Glucose / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy
D002250:Carbon Radioisotopes; D005947:Glucose