title: The effect of desferrioxamine on peroxynitrite-induced oxidative damage in erythrocytes.
abstract: The aim of this study was to investigate the effect of desferrioxamine on peroxynitrite-mediated damage in erythrocytes by measuring the 3-nitrotyrosine level and glutathione peroxidase and Na(+)-K(+) ATPase activities in vitro. 3-Nitrotyrosine levels were determined by HPLC; glutathione peroxidase and Na(+)-K(+) ATPase activities were measured by spectrophotometry. Peroxynitrite increased the 3-nitrotyrosine level but decreased both enzyme activities. In the presence of desferrioxamine, glutathione peroxidase activity was increased with a decrease in the 3-nitrotyrosine level. Desferrioxamine was found to possess an important antioxidant activity as assessed in an in vitro system, reducing protein nitration, restoring enzyme activities and maintaining erythrocyte membrane integrity.
authors.affiliation: Biochemistry Department, Faculty of Medicine, Gazi University, Turkey.
authors.forename: Aytu
authors.initials: A
authors.lastname: Ertabak
authors.forename: Türker
authors.initials: T
authors.lastname: Kutluay
authors.forename: Ali
authors.initials: A
authors.lastname: Unlü
authors.forename: Nurten
authors.initials: N
authors.lastname: Türközkan
authors.forename: Behzat
authors.initials: B
authors.lastname: Cimen
authors.forename: Halil
authors.initials: H
authors.lastname: Yaman
mesh_terms: D003676:Deferoxamine / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D005979:Glutathione Peroxidase / Q000187:drug effects; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects*; D030421:Peroxynitrous Acid / Q000037:antagonists & inhibitors* / Q000633:toxicity; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D030421:Peroxynitrous Acid; C002744:3-nitrotyrosine; D014443:Tyrosine; D005979:Glutathione Peroxidase; D000254:Sodium-Potassium-Exchanging ATPase; D003676:Deferoxamine