The effect of desferrioxamine on peroxynitrite-induced oxidative damage in erythrocytes.
The aim of this study was to investigate the effect of desferrioxamine on peroxynitrite-mediated damage in erythrocytes by measuring the 3-nitrotyrosine level and glutathione peroxidase and Na(+)-K(+) ATPase activities in vitro. 3-Nitrotyrosine levels were determined by HPLC; glutathione peroxidase and Na(+)-K(+) ATPase activities were measured by spectrophotometry. Peroxynitrite increased the 3-nitrotyrosine level but decreased both enzyme activities. In the presence of desferrioxamine, glutathione peroxidase activity was increased with a decrease in the 3-nitrotyrosine level. Desferrioxamine was found to possess an important antioxidant activity as assessed in an in vitro system, reducing protein nitration, restoring enzyme activities and maintaining erythrocyte membrane integrity.
Ertabak
Aytu
A
Biochemistry Department, Faculty of Medicine, Gazi University, Turkey.
Kutluay
Türker
T
Unlü
Ali
A
Türközkan
Nurten
N
Cimen
Behzat
B
Yaman
Halil
H
D003676:Deferoxamine / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D005979:Glutathione Peroxidase / Q000187:drug effects; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects*; D030421:Peroxynitrous Acid / Q000037:antagonists & inhibitors* / Q000633:toxicity; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
D030421:Peroxynitrous Acid; C002744:3-nitrotyrosine; D014443:Tyrosine; D005979:Glutathione Peroxidase; D000254:Sodium-Potassium-Exchanging ATPase; D003676:Deferoxamine