title: Purification and characterization of inulinase from Aspergillus niger AF10 expressed in Pichia pastoris.
abstract: The inuA1 gene encoding an exoinulinase from Aspergillus niger AF10 was expressed in Pichia pastoris, and the recombinant enzyme activity was 316U/ml in a 5L fermentor, with the inulinase protein accounting for 35% of the total protein of fermentation broth. The hydrolysis rate of inulin can reach 92%, with a 25U/g inulin enzyme addition, and 90% of fructose content after 6h. Glucose can significantly inhibit the enzymatic hydrolysis of inulin. This is the first report of glucose inhibition of inulinase-catalyzed hydrolysis.
authors.affiliation: College of Bio and Food Technology, Dalian Institute of Light Industry, Dalian 116034, PR China. dlzlh@163.com
authors.forename: Linghua
authors.initials: L
authors.lastname: Zhang
authors.forename: Changxin
authors.initials: C
authors.lastname: Zhao
authors.forename: Daochen
authors.initials: D
authors.lastname: Zhu
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Ohta
authors.forename: Yunji
authors.initials: Y
authors.lastname: Wang
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D002384:Catalysis; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D010843:Pichia / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; C023086:inulinase