Plasmid partitioning and the spreading of P1 partition protein ParB.
Bacterial plasmids of low copy number, P1 prophage among them, are actively partitioned to nascent daughter cells. The process is typically mediated by a pair of plasmid-encoded proteins and a cis-acting DNA site or cluster of sites, referred to as the plasmid centromere. P1 ParB protein, which binds to the P1 centromere (parS), can spread for several kilobases along flanking DNA. We argue that studies of mutant ParB that demonstrated a strong correlation between spreading capacity and the ability to engage in partitioning may be misleading, and describe here a critical test of the dependence of partitioning on the spreading of the wild-type protein. Physical constraints imposed on the spreading of P1 ParB were found to have only a minor, but reproducible, effect on partitioning. We conclude that, whereas extensive ParB spreading is not required for partitioning, spreading may have an auxiliary role in the process.
Rodionov
Oleg
O
Laboratory of Biochemistry, National Cancer Institute, NIH, Bldg 37, Room 6044C, 37 Convent Drive, Bethesda, MD 20892-4255, USA.
Yarmolinsky
Michael
M
D001426:Bacterial Proteins / Q000378:metabolism; D017101:Bacteriophage P1 / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D002455:Cell Division; D004268:DNA-Binding Proteins / Q000378:metabolism; D010957:Plasmids / Q000502:physiology*; D014764:Viral Proteins / Q000378:metabolism*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C121785:ParB protein, Enterobacteria phage P1; D014764:Viral Proteins