title: [Determination of aloin in aloes by HPLC].
abstract: OBJECTIVE
  To describe a simple and rapid isocratic reversed-phase high performance liquid chromatography method for the baseline separation, identification and assay of aloin in aloes.
  
  
  METHODS
  The analytical column was a ZORBAX SB-C18(4.6 mm x 250 mm) filled with a 5 microns stationary phase. The mobile phase consisted of acetonitrile-water (25:75); the flow-rate was 1 mL.min-1. The injection volume was 10 microL. The DAD detector was set at 355 nm.
  
  
  RESULTS
  The calibration curve was linear over the range of 0.17-5.9 micrograms (r = 0.9999). The average recovery of the method was 98.6%, RSD 1.32% (n = 6).
  
  
  CONCLUSIONS
  The results showed that this method was reliable and accurate. The method was applied to eleven Cape and East African aloes of different origin.
authors.affiliation: Institute for Drug and Instrument Control of PLA, Beijing 100071, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000504:Aloe / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004642:Emodin / Q000031:analogs & derivatives / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D004642:Emodin; C006457:alloin