Induction of heat shock protein 72 in the failing heart is attenuated after an exposure to heat shock.
Induction of heat shock protein (Hsp) 72 in the right ventricular muscle of the rat with heart failure following acute myocardial infarction (AMI) was examined. AMI was induced by the left coronary artery ligation (CAL). The animals at the 8th, but not 2nd, week after CAL revealed a decrease in cardiac output index (COI), suggesting that heart failure had developed by 8 weeks after CAL. Increases in the right ventricular developed pressure and the ratios of right ventricle/body weight and lung/body weight at the 2nd and 8th weeks showed the development of the right ventricular hypertrophy. After measurement of hemodynamic parameters, the hearts isolated from animals at the 2nd and 8th weeks after CAL (2w- and 8w-CAL hearts, respectively) were perfused and subjected to heat shock (at 42 degrees C, for 15 min) followed by 6-h perfusion. At the end of perfusion, Hsp72 content in the left ventricle without infarct area (viable LV) and the right ventricle (RV) was determined by the Western immunoblotting method. The production of myocardial Hsp72 in the viable LV and RV of the 2w-CAL heart increased after an exposure to heat shock. In contrast, induction of Hsp72 in the viable LV and RV of the 8w-CAL heart was blunted. The results suggest that the development of heart failure following AMI may result in a decrease in the ability for Hsp72 induction not only in the viable LV but also in the RV, leading to contractile dysfunction of the heart.
Tanonaka
Kouichi
K
Department of Pharmacology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan.
Toga
Wakako
W
Takahashi
Masaya
M
Yoshida
Hiroyuki
H
Oikawa
Ryo
R
Takeo
Satoshi
S
D000818:Animals; D002303:Cardiac Output, Low / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation; D050884:HSP72 Heat-Shock Proteins; D006352:Heart Ventricles / Q000378:metabolism* / Q000473:pathology; D006360:Heat-Shock Proteins; D018869:Heat-Shock Response / Q000502:physiology; D006358:Hot Temperature; D066298:In Vitro Techniques; D008297:Male; D009200:Myocardial Contraction; D009203:Myocardial Infarction / Q000378:metabolism* / Q000473:pathology; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D018497:Ventricular Dysfunction, Right
D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins