Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method.
Paracoccidioidomycosis is a deep mycosis caused by the thermo-dependent dimorphic fungus Paracoccidioides brasiliensis and is prevalent in Latin American countries. We detected the species specific gp43 gene of P. brasiliensis by loop-mediated isothermal amplification (LAMP) in 22 clinical and seven armadillo-derived isolates. The amplified DNA appeared as a ladder with a specific banding pattern. The advantage of the LAMP method is speed; only 3 h were necessary for identification of the organism and diagnosis of the disease. We were also able to obtain positive results from DNA extracted from a paraffin-embedded tissue sample of paracoccidioidomycosis, suggesting that this method may achieve clinical application in the near future.
Endo
Shigeo
S
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
Komori
Takashi
T
Ricci
Giannina
G
Sano
Ayako
A
Yokoyama
Koji
K
Ohori
Akira
A
Kamei
Katsuhiko
K
Franco
Marcello
M
Miyaji
Makoto
M
Nishimura
Kazuko
K
D000818:Animals; D000946:Antigens, Fungal / Q000235:genetics*; D001135:Armadillos / Q000382:microbiology; D016172:DNA Fingerprinting / Q000379:methods; D004271:DNA, Fungal / Q000032:analysis; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010228:Paracoccidioides / Q000235:genetics* / Q000302:isolation & purification; D010229:Paracoccidioidomycosis / Q000175:diagnosis / Q000382:microbiology; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods
C103180:43 kDa protein, Paracoccidioides; D000946:Antigens, Fungal; D004271:DNA, Fungal; D005656:Fungal Proteins; D006023:Glycoproteins