Incorporation of arachidonic and linoleic acid hydroperoxides into cultured human umbilical vein endothelial cells.
The current study assessed the differential incorporation of 12-hydroperoxyeicosatetraenoic acid (12-HPETE), arachidonic acid (AA), 12-hydroxyeicosatetraenoic acid (12-HETE) and the linoleic acid (LA) oxidation products, 13-hydroxyoctadecadienoic acid (13-HODE) and 13-hydroperoxyoctadecadienoic acid (13-HPODE), into human umbilical vein endothelial cells (HUVEC). Approximately 80-90% of AA (10(-8)-10(-5)M) and 80% of LA (10(-8)-10(-5)M) were incorporated into HUVEC within 12h, while less than 50% of the hydroxy metabolites (12-HETE, 12-HPETE, 13-HODE, 13-HPODE) were incorporated into HUVEC over 48h. Further, treatment of HUVEC with either 12-HPETE or 13-HPODE (concentrations of 10(-5)M) had no effect on cell number at a 48h time point when compared with control. These results demonstrate that exogeneous hydroxy metabolites are incorporated into HUVEC to a lesser degree than were endogenous fatty acids. Further, we speculate that 12-HPETE and 13-HPODE are rapidly metabolized to substances without significant cytotoxic effects.
Tanabe
Michiharu
M
Department of Neurosurgery, Institute of Neurological Sciences, Tottori University School of Medicine, 36-1 Nishi-machi, Yonago 683-8504, Japan.
Tabuchi
Sadaharu
S
Iwatsuki
Masato
M
Kurosaki
Masamichi
M
Kamitani
Hideki
H
Yokota
Masayuki
M
Watanabe
Takashi
T
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid / Q000378:metabolism* / Q000633:toxicity; D016718:Arachidonic Acid / Q000378:metabolism*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D006801:Humans; D015289:Leukotrienes / Q000378:metabolism* / Q000633:toxicity; D008041:Linoleic Acids / Q000378:metabolism* / Q000633:toxicity; D008054:Lipid Peroxides / Q000378:metabolism* / Q000633:toxicity; D014471:Umbilical Veins / Q000166:cytology
D015289:Leukotrienes; D008041:Linoleic Acids; D008054:Lipid Peroxides; C038649:13-hydroperoxy-9,11-octadecadienoic acid; C008285:linoleic acid hydroperoxide; D016718:Arachidonic Acid; C024348:13-hydroxy-9,11-octadecadienoic acid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; C021201:12-HPETE