title: [Study of the cholinesterase active site using a fluorescent probe].
abstract: We studied fluorescence of 9-amino-1,2,3,4-tetrahydroacridine hydrochloride (tacrine) in the presence of serum cholinesterase. Quenching of tacrine fluorescence dependent on cholinesterase activity has been revealed. A quenching mechanism is proposed; it includes formation of a complex and charge transfer mediated by excited tacrine molecule as well as indole of tryptophan residue from the periphery of cholinesterase active site.
authors.affiliation: Faculty of Physics, Moscow State University, Vorob'evy gory, Moscow, 119992 Russia.
authors.forename: D O
authors.initials: DO
authors.lastname: Vetkin
authors.forename: E T
authors.initials: ET
authors.lastname: Gaĭnullina
authors.forename: V A
authors.initials: VA
authors.lastname: Karavaev
authors.forename: R N
authors.initials: RN
authors.lastname: Nurmukhametov
authors.forename: S B
authors.initials: SB
authors.lastname: Ryzhikov
authors.forename: V F
authors.initials: VF
authors.lastname: Taranchenko
mesh_terms: D000609:Aminoacridines / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002800:Cholinesterase Inhibitors / Q000378:metabolism*; D002802:Cholinesterases / Q000097:blood / Q000737:chemistry / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007211:Indoles / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013619:Tacrine / Q000378:metabolism*; D014364:Tryptophan / Q000737:chemistry
chemical_list: D000609:Aminoacridines; D002800:Cholinesterase Inhibitors; D005456:Fluorescent Dyes; D007211:Indoles; D013619:Tacrine; D014364:Tryptophan; D002802:Cholinesterases; C025902:acridine yellow