title: Morphological changes contribute to apoptotic cell death and are affected by caspase-3 and caspase-6 inhibitors during red sea bream iridovirus permissive replication.
abstract: Red sea bream iridovirus (RSIV) of the Iridoviridae family is a causative agent of lethal infections in many cultured marine fish species in southwestern Japan. RSIV-induced apoptosis was divided as follows: (1). cell shrinkage and rounding at the early apoptotic stage, (2). cell enlargement at the middle apoptotic stage, (3). formation of apoptotic body-like vesicles at the late apoptotic stage and phagocytosis by neighboring cells, and (4). loss of membrane integrity in apoptotic body-like vesicles without phagocytosis by neighboring cells. By affinity labeling, RSIV-induced apoptosis included caspase-dependent apoptosis. RSIV infection caused cell rounding but not cell enlargement or formation of apoptotic body-like vesicles and further restricted part of the structural protein synthesis in the presence of caspase-3 and -6 inhibitors. These findings showed the involvement of caspase-3 and -6 in the morphological changes at the middle and late apoptotic stages and viral protein synthesis in the late stage of RSIV infection.
authors.affiliation: Department of Aquaculture, Kochi University, Nankoku, Kochi 783-8502, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Imajoh
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Sugiura
authors.forename: Syun-ichirou
authors.initials: S
authors.lastname: Oshima
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3; D053178:Caspase 6; D061945:Caspase Inhibitors; D020169:Caspases / Q000378:metabolism*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D003588:Cytopathogenic Effect, Viral; D053938:DNA Fragmentation; D017994:Iridovirus / Q000472:pathogenicity* / Q000502:physiology; D008854:Microscopy, Electron; D021541:Sea Bream / Q000821:virology*; D014779:Virus Replication
chemical_list: D061945:Caspase Inhibitors; D015853:Cysteine Proteinase Inhibitors; D053148:Caspase 3; D053178:Caspase 6; D020169:Caspases