title: Morphologically structured model for antitumoral retamycin production during batch and fed-batch cultivations of Streptomyces olindensis.
abstract: A morphologically structured model is proposed to describe trends in biomass growth, substrate consumption, and antitumoral retamycin production during batch and fed-batch cultivations of Streptomyces olindensis. Filamentous biomass is structured into three morphological compartments (apical, subapical, and hyphal), and the production of retamycin, a secondary metabolite, is assumed to take place in the subapical cell compartment. Model accounts for the effect of glucose as well as complex nitrogen source on both the biomass growth and retamycin production. Laboratory data from bench-scale batch and fed-batch fermentations were used to estimate some model parameters by nonlinear regression. The predictive capability of the model was then tested for additional fed-batch and continuous experiments not used in the previous fitting procedure. The model predictions show fair agreement to the experimental data. The proposed model can be useful for further studies on process optimization and control.
authors.affiliation: Department of Chemical Engineering--Escola Politécnica da Universidade de São Paulo, P.O. Box 61548, CEP 05424-970, São Paulo, SP, Brazil. rgiudici@usp.br
authors.forename: Reinaldo
authors.initials: R
authors.lastname: Giudici
authors.forename: Celso R D
authors.initials: CR
authors.lastname: Pamboukian
authors.forename: Maria Cândida R
authors.initials: MC
authors.lastname: Facciotti
mesh_terms: D018943:Anthracyclines / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000378:metabolism; D002455:Cell Division / Q000502:physiology; D003470:Culture Media; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D007218:Industrial Microbiology / Q000295:instrumentation / Q000379:methods*; D008433:Mathematics; D008954:Models, Biological; D008962:Models, Theoretical; D009584:Nitrogen / Q000378:metabolism; D015203:Reproducibility of Results; D013302:Streptomyces / Q000254:growth & development / Q000378:metabolism*; D013997:Time Factors
chemical_list: D018943:Anthracyclines; D000970:Antineoplastic Agents; D003470:Culture Media; C002920:retamycin; D005947:Glucose; D009584:Nitrogen