Changes in mitochondrial DNA copy number in blood cells from HIV-infected patients undergoing antiretroviral therapy.
Mitochondrial DNA (mtDNA) copy number was measured in peripheral blood mononuclear cells (PBMCs) from 69 individuals using a real-time NASBA quantitative assay. Patients with HIV infection harbored significantly lower mtDNA copy number in PBMC than HIV-negative controls. Besides, subjects on stavudine-containing regimens showed significantly lower median mtDNA amounts than HIV-positive patients receiving other antiretroviral drugs, and this was associated with higher lactate levels. Thus, either HIV infection itself or treatment with stavudine-containing regimens might induce mtDNA depletion and related metabolic disturbances as hyperlactatemia.
de Mendoza
Carmen
C
Service of Infectious Diseases, Hospital Carlos III, Madrid, Spain.
de Ronde
Anthony
A
Smolders
Karlijn
K
Blanco
Francisco
F
Garcia-Benayas
Teresa
T
de Baar
Michel
M
Fernández-Casas
Paloma
P
González-Lahoz
Juan
J
Soriano
Vincent
V
D019380:Anti-HIV Agents / Q000009:adverse effects* / Q000627:therapeutic use; D004272:DNA, Mitochondrial / Q000097:blood* / Q000235:genetics; D004359:Drug Therapy, Combination; D018628:Gene Dosage; D015658:HIV Infections / Q000188:drug therapy*; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics; D006801:Humans; D007773:Lactates / Q000097:blood; D018894:Reverse Transcriptase Inhibitors / Q000009:adverse effects* / Q000627:therapeutic use; D021161:Self-Sustained Sequence Replication / Q000379:methods; D018119:Stavudine / Q000009:adverse effects* / Q000627:therapeutic use
D019380:Anti-HIV Agents; D004272:DNA, Mitochondrial; D007773:Lactates; D018894:Reverse Transcriptase Inhibitors; D018119:Stavudine