Detection of polyomavirus DNA in human brain tumors.
OBJECTIVE
To investigate surgically resected human brain tumors for the presence of polyomavirus DNA.


METHODS
65 samples of surgically resected human brain tumors (57 tumors were of neuroectodermal genesis, 4--mesenchimal, 4--metastatic tumors) were examined for the presence of viral DNA by PCR analysis.


RESULTS
DNA of polyomaviruses was found in oligodendroligomas (66.7% of cases), oligodendroastrocytomas (66.7% of cases) and glioblastomas (40% of cases). In metastatic tumors viral infection had not been detected. No correlation between the grade of brain tumors and infection with polyomavirus DNA has been revealed.


CONCLUSIONS
Our data point to importance of investigation of the brain tumors and cerebrospinal fluid for determination of viral infection.
Vasilyeva
Iryna G
IG
A.P. Romodanov Institute of Neurosurgery of Academy of Medical Sciences of Ukraine, Kyiv, Ukraine. ira@neuro.kiev.ua
Shamaev
Mikhail I
MI
Glavatskiy
Alexandr Ya
AY
Chopick
Natalya G
NG
Olexenko
Natalya P
NP
Tsyubko
Olga I
OI
Galanta
Elena S
ES
Malisheva
Tatyana A
TA
D001932:Brain Neoplasms / Q000473:pathology / Q000821:virology*; D002555:Cerebrospinal Fluid / Q000821:virology; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011120:Polyomavirus / Q000235:genetics / Q000302:isolation & purification*; D027601:Polyomavirus Infections / Q000473:pathology / Q000821:virology*; D014412:Tumor Virus Infections / Q000473:pathology / Q000821:virology*
D004279:DNA, Viral