Microdialysis combined with liquid chromatography-tandem mass spectrometry for the determination of 6-aminobutylphthalide and its main metabolite in the brains of awake freely-moving rats.
6-Aminobutylphthalide (ABP) is a new drug candidate which is currently being developed for the treatment of cerebral ischemia. The pharmacokinetics and metabolism of ABP were studied using in situ microdialysis sampling in the brains of awake freely-moving rats. Two LC-MS/MS methods were used for the quantitative and qualitative analysis of microdialysate. For comparison and confirmation, brain tissue samples were also analyzed by LC-MS/MS and GC/MS. The results described provide more authentic information in pharmacokinetics and metabolism at the site of action by using the coupling of microdialysis to LC-MS/MS technique than the traditional sampling methods.
Qiao
Jin-ping
JP
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, 1 Xian Nong Tan Street, Beijing 100050, China.
Abliz
Zeper
Z
Chu
Feng-ming
FM
Hou
Pei-ling
PL
Zhao
Li-yan
LY
Xia
Min
M
Chang
Yan
Y
Guo
Zong-ru
ZR
D000818:Animals; D001572:Benzofurans / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D008297:Male; D017551:Microdialysis; D051381:Rats; D017208:Rats, Wistar
C493999:6-Aminobutylphthalide; D001572:Benzofurans