title: [Studies on liver-toxicity in rhigoma of Dioscorea bulbifera].
abstract: OBJECTIVE
  To explore the liver-toxic fraction in Rhigoma of Dioscorea bulbifera.
  
  
  METHODS
  The rats were randomized into four groups: control group (20% PVP-water), T001(10% total methanol extraction), F002(5% chloroform fraction) and F003(5% methanol fraction). Direct bilirubin (DBil) and Glutamic-pyruvic transaminase (GPT) were examined, and liver index was measured. The histological and morphological observations were performed with optical and electrical microscope.
  
  
  RESULTS
  T001 and F002 showed significant liver toxicity.
  
  
  CONCLUSIONS
  The chloroform fraction was the liver-toxic fraction of D. bulbifera.
authors.affiliation: NO. 98 Hospital of PLA, Huzhou 313000, Zhejang, China. tanxq@sohu.com
authors.forename: Xing-qi
authors.initials: XQ
authors.lastname: Tan
authors.forename: Jin-lan
authors.initials: JL
authors.lastname: Ruan
authors.forename: Hai-sheng
authors.initials: HS
authors.lastname: Chen
authors.forename: Ju-ying
authors.initials: JY
authors.lastname: Wang
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001663:Bilirubin / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000209:etiology / Q000473:pathology; D027742:Dioscorea / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000633:toxicity*; D005260:Female; D008099:Liver / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal; D000410:Alanine Transaminase; D001663:Bilirubin