Detection of Candida albicans mRNA in archival histopathology samples by reverse transcription-PCR.
The feasibility of detecting Candida albicans mRNA in formalin-fixed paraffin-embedded archival human histopathology specimens by reverse transcription-PCR (RT-PCR) was investigated. RT with gene-specific primers was used to detect five single-copy C. albicans gene transcripts, including those of two housekeeping genes, in oral candidiasis samples up to 8 years of age.
Beggs
Kyle T
KT
Department of Oral Sciences, School of Dentistry, University of Otago, Dunedin 9001, New Zealand.
Holmes
Ann R
AR
Cannon
Richard D
RD
Rich
Alison M
AM
D001483:Base Sequence; D002176:Candida albicans / Q000235:genetics* / Q000302:isolation & purification; D002180:Candidiasis, Oral / Q000175:diagnosis / Q000473:pathology / Q000821:virology; D017931:DNA Primers / Q000235:genetics; D005557:Formaldehyde; D018628:Gene Dosage; D005800:Genes, Fungal; D006801:Humans; D016612:Paraffin Embedding; D012331:RNA, Fungal / Q000235:genetics* / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D016707:Tissue Fixation; D014773:Virology / Q000379:methods
D017931:DNA Primers; D012331:RNA, Fungal; D012333:RNA, Messenger; D005557:Formaldehyde