Therapeutic effects of glucose infusion on monochloroacetic acid exposure in rats.
The effects of glucose infusion on monochloroacetate (MCA) exposure were examined in male rats with a view toward effective clinical treatment for MCA intoxication. Rats were injected with 80 mg/kg sodium monochloroacetate (SMCA) (single lethal dose) and then infused with saline (control group) or 5% or 10% glucose solution at 2 mL/hour for ten hours. No animal in the control group survived the total 14-day follow-up period. The survival rate in 5% glucose group was 57% at ten hours; it decreased to 14% at 14 days. The survival rate in 10% glucose group was 79% at ten hours, and all rats that survived the first ten hours also survived the 14 days. Kaplan-Meier analysis showed the survival rate in 10% glucose group to be improved upon in both the 5% glucose group and the control group. Blood glucose and lactate levels were measured every hour during infusion. Blood glucose levels decreased in the control group but remained in the glucose-infused groups. Although the blood lactate level increased in each group, there was an excellent inverse linear relation between blood glucose levels and blood lactate levels. Thus, continuous parenteral infusion of glucose solution at an early stage after exposure may be an effective clinical therapy for the prevention of hypoglycaemia and metabolic lactic acidosis caused by MCA.
Shimizu
Hiroyasu
H
Department of Hygiene and Public Health, Osaka Medical College, Takatsuki, Osaka, Japan.
Dote
Tomotaro
T
Usuda
Kan
K
Toshina
Yumi
Y
Kato
Junko
J
Sakai
Akiko
A
Furuya
Eisuke
E
Kono
Koichi
K
D000085:Acetates / Q000633:toxicity*; D000140:Acidosis, Lactic / Q000517:prevention & control*; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D005947:Glucose / Q000008:administration & dosage*; D007003:Hypoglycemia / Q000517:prevention & control*; D007262:Infusions, Intravenous; D019344:Lactic Acid / Q000097:blood; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015996:Survival Rate
D000085:Acetates; D001786:Blood Glucose; D019344:Lactic Acid; C006972:chloroacetic acid; D005947:Glucose