title: Detection of Naegleria sp. in a thermal, acidic stream in Yellowstone National Park.
abstract: An initial survey of sequences of PCR-amplified portions of the 18S rRNA genes from a community DNA clone library, prepared from an algal mat in a thermal, acidic stream in Yellowstone National Park, WY, USA, revealed among other sequences, several that matched Vahlkampfia. This finding prompted further investigation using primers specific for Naegleria. Sequences from a subsequent DNA clone library, prepared from the 5.8S rRNA gene and the adjacent internal transcribed spacer (ITS) regions of the rRNA, closely matched Naegleria and formed an independent lineage within a clade containing Naegleria sturti and Naegleria niuginiensis. The sequences may represent a new Naegleria species.
authors.affiliation: Thermal Biology Institute, Montana State University, Bozeman, Montana 59717, USA. umbks@montana.edu
authors.forename: Kathy B
authors.initials: KB
authors.lastname: Sheehan
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Ferris
authors.forename: Joan M
authors.initials: JM
authors.lastname: Henson
mesh_terms: D000818:Animals; D056890:Eukaryota / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D045482:Hot Springs / Q000382:microbiology*; D009253:Naegleria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000032:analysis; D014871:Water Microbiology
chemical_list: D012335:RNA, Ribosomal