title: Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli.
abstract: The gene encoding the excretory-secretory antigen TES-120 of dog ascarid worm Toxocara canis was cloned into the bacterium Escherichia coli. The specificity of the recombinant TES-120 antigen produced by the bacterium was investigated. A total of 45 human serum samples from patients infected with differenthelminthes and protozoa, including 8 cases of toxocariasis, were tested against the recombinant antigens in immunoblot assays. The results from the assays revealed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis.
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. fongmy@um.edu.my
authors.forename: Mun-Yik
authors.initials: MY
authors.lastname: Fong
authors.forename: Yee-Ling
authors.initials: YL
authors.lastname: Lau
authors.forename: Ithoi
authors.initials: I
authors.lastname: Init
authors.forename: I
authors.initials: I
authors.lastname: Jamaiah
authors.forename: A Khairul
authors.initials: AK
authors.lastname: Anuar
authors.forename: N
authors.initials: N
authors.lastname: Rahmah
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000378:metabolism; D003001:Cloning, Molecular; D004285:Dogs; D004926:Escherichia coli / Q000378:metabolism; D015801:Helminth Proteins / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D007815:Larva Migrans / Q000175:diagnosis* / Q000276:immunology; D011994:Recombinant Proteins / Q000378:metabolism; D012680:Sensitivity and Specificity; D017166:Toxocara canis / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins; D011994:Recombinant Proteins; C045159:TES-26 protein, Toxocara canis