Functional characterization of human Tc0, Tc1 and Tc2 CD8+ T cell clones: control of X4 and R5 HIV strain replication.
CD8(+) T lymphocytes have the potential ability to inhibit human immunodeficiency virus (HIV) replication, by secreting soluble(s) factor(s) known as CD8(+) T lymphocyte antiviral factor (CAF). A panel of CD8(+) and CD4(+) T cell clones from HIV1-infected and uninfected donors were generated to better define the phenotype of CAF-producing cells. We first verified that the different CD4(+) T cell subsets (Th0, Th1, and Th2) were productively infected by X4 and R5 virus strains. X4 viral replication in CD4(+) T cells was controlled by the three CD8(+) T cell subsets (Tc0, Tc1, and Tc2); however, the frequency of Tc clones controlling R5 strain was much lower with a dramatic absence of this activity among Tc clones from uninfected donor. Finally, capacity to control viral replication showed an heterogeneity: some clones could control both virus strains, some controlled only the X4 virus, whereas the majority exerted no suppressive activity.
Février
Michèle
M
Unité d'Immunopathologie Virale, URA CNRS 1930, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris cedex 15, France. mfevrier@pasteur.fr
le Borgne
Sylvie
S
Marty
Christian
C
Talarmin
Antoine
A
Rivière
Yves
Y
D002999:Clone Cells; D016207:Cytokines / Q000276:immunology; D005260:Female; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D008297:Male; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D018417:Th1 Cells / Q000821:virology; D018418:Th2 Cells / Q000821:virology; D014779:Virus Replication
D016207:Cytokines; D012194:RNA-Directed DNA Polymerase