Identification of a Shiga-toxin type I variant containing an IS1203-like element, from Shiga-toxin producing Escherichia coli O157:H7.
We found two Shiga toxin producing Escherichia coli O157:H7 strains isolated from humans carrying the stx(1) gene with an IS1203-like element (designated as IS1203v(1)). The IS1203v(1) was inserted into the coding region of the A subunit 7 bp upstream from the TGA termination codon, resulting in a loss of two amino acid residues (Ser-Ser) from its C terminus. Toxicity of the Stx1 was confirmed by Vero cell assay. IS1203v(1) hardly affected the stx(1) gene in either its expression or the toxicity of its product.
Suzuki
Masahiro
M
Aichi Prefectural Institute of Public Health, 7-6 Nagare, Tsuji-machi, Kita-ku, Nagoya 462-8576, Japan. masahiro_4_suzuki@pref.aichi.lg.jp
Kondo
Fumio
F
Ito
Yuko
Y
Matsumoto
Masakado
M
Hata
Mami
M
Oka
Hisao
H
Takahashi
Masao
M
Sakae
Kenji
K
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D002851:Chromatography, High Pressure Liquid; D018388:Codon, Terminator; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D019453:Escherichia coli O157 / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D021122:Protein Subunits / Q000235:genetics; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D022622:Shiga Toxin 1 / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000633:toxicity; D014709:Vero Cells
D018388:Codon, Terminator; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D021122:Protein Subunits; D022622:Shiga Toxin 1