title: A keratin K5Cre transgenic line appropriate for tissue-specific or generalized Cre-mediated recombination.
abstract: We describe here a mouse line bearing a bovine keratin K5Cre recombinase transgene. These mice showed a dual pattern of Cre-mediated recombination, depending on the parent transmitting the transgene. In paternal transmission, recombination occurred specifically in the skin and stratified epithelia-as expected according to the expression of endogenous keratin K5. However, constitutive recombination between loxP sites transmitted by the sperm took place when the mother possessed the K5Cre transgene, even when the transgene was absent in the progeny. Cre expression in late-stage oocytes, with the Cre protein persisting into the developing embryo, leads to the constitutive recombination observed. Thus, this transgenic line allows for both tissue-specific and generalized recombination, depending on the breeding scheme.
authors.affiliation: Epithelial Damage, Repair and Tissue Engineering Program, CIEMAT, Madrid, Spain.
authors.forename: Angel
authors.initials: A
authors.lastname: Ramirez
authors.forename: Angustias
authors.initials: A
authors.lastname: Page
authors.forename: Alberto
authors.initials: A
authors.lastname: Gandarillas
authors.forename: Jennifer
authors.initials: J
authors.lastname: Zanet
authors.forename: Sophie
authors.initials: S
authors.lastname: Pibre
authors.forename: Miguel
authors.initials: M
authors.lastname: Vidal
authors.forename: Laura
authors.initials: L
authors.lastname: Tusell
authors.forename: Anna
authors.initials: A
authors.lastname: Genesca
authors.forename: Duncan A
authors.initials: DA
authors.lastname: Whitaker
authors.forename: David W
authors.initials: DW
authors.lastname: Melton
authors.forename: Jose L
authors.initials: JL
authors.lastname: Jorcano
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D017931:DNA Primers; D005455:Fluorescent Antibody Technique; D005838:Genotype; D007633:Keratins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D045522:Recombinases / Q000378:metabolism*; D011995:Recombination, Genetic
chemical_list: D017931:DNA Primers; D045522:Recombinases; D007633:Keratins