High-performance thin-layer chromatography with a derivatization procedure, a suitable method for the identification and the quantitation of busulfan in various pharmaceutical products.
Busulfan is an alkylating agent widely used in combination chemotherapy regimens followed by allogeneic or autologous hematopoietic stem cell transplantation (HSCT). We present a rapid method for assaying busulfan in pharmaceutical preparations using high-performance thin-layer chromatography (HPTLC) and derivatization with 4-nitrobenzylpyridine. The method is accurate and precise and allows quantitation of busulfan in aqueous solutions from 100 to 500 microg/ml. It is suitable for identification. quantitation and stability studies of busulfan in pharmaceutical products, i.e. capsules or infusion bags prepared in a hospital pharmacy.
Bouligand
Jérôme
J
Department of Clinical Pharmacy, Institut Gustave Roussy, 39 Rue Camille Desmoulins, 94800 Villejuif, France.
Paci
Angelo
A
Mercier
Lionel
L
Vassal
Gilles
G
Bourget
Philippe
P
D002066:Busulfan / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000737:chemistry
D004364:Pharmaceutical Preparations; D002066:Busulfan