Comparison of short- and long-duration oxygen treatment after cerebral asphyxia in newborn piglets.
We tested whether reoxygenation with 100% O(2) for 5 min after experimental asphyxia in newborn piglets was as efficient as 100% O(2) for 20 min compared with room air. Forty-one anesthetized piglets, 1-3 d old, were randomized to cerebral hypoxemia-ischemia-hypercapnia (HIH) or control (n = 5). HIH was achieved by ventilation with 8% O(2), temporary occlusion of the common carotid arteries, and adding of CO(2). After 25 min, reoxygenation-reperfusion was started with 100% O(2) for 20 min (group 1, n = 12), 100% O(2) for 5 min (group 2, n = 12), or 21% O(2) (group 3, n = 12). All piglets were observed for 2 h. During reoxygenation-reperfusion, significantly higher blood pressure and more complete restoration of microcirculation (laser Doppler flow) in the cerebral cortex was found in both groups reoxygenated with 100% O(2) compared with 21% O(2) (regional cerebral blood flow >or=100% versus 70% of baseline, p = 0.04). Reoxygenation with 100% O(2) for 5 min was as efficient as 20 min. Oxygen delivery in cortex was significantly higher in groups 1 and 2 compared with group 3 (p = 0.03), but there were no significant differences in cerebral metabolic rate for oxygen. In the striatum, no significant differences in flow or extracellular glutamate, glycerol, and lactate/pyruvate ratio were found between the groups. In conclusion, after experimental asphyxia, newborn piglets can be reoxygenated as efficiently with 100% O(2) for only 5 min as 100% O(2) for 20 min compared with room air.
Solas
Anne-Beate
AB
Department of Pediatric Research, The National Hospital, Oslo, Norway. absolaas@klinmed.uio.no
Munkeby
Berit Holte
BH
Saugstad
Ola Didrik
OD
D000818:Animals; D000831:Animals, Newborn; D001237:Asphyxia / Q000188:drug therapy*; D001794:Blood Pressure; D002245:Carbon Dioxide / Q000097:blood; D002540:Cerebral Cortex / Q000098:blood supply / Q000378:metabolism; D002560:Cerebrovascular Circulation; D003342:Corpus Striatum / Q000098:blood supply / Q000378:metabolism; D004195:Disease Models, Animal; D018698:Glutamic Acid / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D017078:Laser-Doppler Flowmetry; D017551:Microdialysis; D010100:Oxygen / Q000097:blood / Q000494:pharmacology*; D019289:Pyruvic Acid / Q000378:metabolism; D013552:Swine
D002245:Carbon Dioxide; D019344:Lactic Acid; D018698:Glutamic Acid; D019289:Pyruvic Acid; D005990:Glycerol; D010100:Oxygen