New insights on glycerol transport in Saccharomyces cerevisiae.
Previous studies evidenced in Saccharomyces cerevisiae the activity of a H(+)/glycerol symport, derepressed by growth on non-fermentable carbon sources, later associated with GUP1 and GUP2 genes. It was also demonstrated that only the combined deletion of GUP1, GUP2 together with GUT1 (glycerol kinase) abolished active transport in ethanol-induced cells. In this work, we show that a glycerol H(+)/symport, with identical characteristics to the previously described, was found in gup1gup2gut1 grown under salt-stress, particularly high in cells collected during diauxic-shift. These results suggest different roles for Gup1/2p than glycerol transport. The gene encoding for glycerol active uptake is thus yet unknown.
Neves
Luisa
L
Department of Biology, Environmental Science Research Center, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Lages
Fernanda
F
Lucas
Cândida
C
D001692:Biological Transport; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005990:Glycerol / Q000378:metabolism* / Q000493:pharmacokinetics; D005991:Glycerol Kinase / Q000378:metabolism; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D002352:Carrier Proteins; C499609:GUP2 protein, S cerevisiae; C414103:Gup1 protein, S cerevisiae; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; C487106:GUT1 protein, S cerevisiae; D005991:Glycerol Kinase; D005990:Glycerol