title: [DNA family shuffling of tissue-type plasminogen activator genes].
abstract: OBJECTIVE
  To probe the possibility of screening tPA with higher activity through DNA shuffling.
  
  
  METHODS
  Human, rat and rhesus monkey tPA cDNAs were used as the templates of "DNA family shuffling". The diverse tPA library was expressed in CHO cells, then the screening was carried out.
  
  
  RESULTS
  Two shuffled clones, t9 and t17 were selected. The activity of tPA expressed by t9 clone was 4-fold higher than the activity of human tPA. tPA expressed by t17 clone was found having a 88-amino-acid deletion, but still showed the same activity as human tPA. DNA sequencing demonstrated that the sequences of t9 and t17 were mainly derived from human and monkey tPA cDNAs.
  
  
  CONCLUSIONS
  Above work has laid the foundation for further experiments in the screening for higher activity of tPA.
authors.affiliation: Institute of Microbiology and Epidemiology, AMMS, Beijing 100071, China.
authors.forename: Qing-sheng
authors.initials: QS
authors.lastname: Huang
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Jun-jie
authors.initials: JJ
authors.lastname: Xu
authors.forename: Yi-gang
authors.initials: YG
authors.lastname: Tong
authors.forename: Hai-tao
authors.initials: HT
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D032621:DNA Shuffling; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D008253:Macaca mulatta; D051381:Rats; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D010959:Tissue Plasminogen Activator