Qualitative and quantitative difference in mutation induction between carbon- and neon-ion beams in normal human cells.
We investigated the difference in cell-killing effect and mutation induction between carbon- and neon-ion beams in normal human cells. Carbon- and neon-ion beams were accelerated by the Riken Ring Cyclotron (RRC) at the Institute of Physical and Chemical Research in Japan. Cell-killing effect was measured as the reproductive cell death using the colony formation assay. Mutation induction at the HPRT locus was detected to measure 6-thioguanine-resistant clones. The mutation spectrum of the deletion pattern of exons of induced mutants was analyzed using the multiplex polymerase chain reaction (PCR). Cell-killing effect was almost the same between carbon- and neon-ion beams with similar linear energy transfer (LET) values, while there observed a large difference in mutation frequency. Furthermore, in the case of neon-ion beams 60% of mutants showed total deletions and 35-40% showed partial deletions, while 95-100% of carbon-ion induced mutants showed total deletions. The results suggest that different ion species may cause qualitative and quantitative difference in mutation induction even if the LET values are similar.
Suzuki
Masao
M
International Space Radiation Laboratory, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba-shi 263, Japan. m_suzuki@nirs.go.jp
Tsuruoka
Chizuru
C
Kanai
Tatsuaki
T
Kato
Takeshi
T
Yatagai
Fumio
F
Watanabe
Masami
M
D002244:Carbon; D016923:Cell Death / Q000528:radiation effects*; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects*; D002588:Cesium Radioisotopes; D017357:Cyclotrons; D004307:Dose-Response Relationship, Radiation; D004622:Embryo, Mammalian / Q000166:cytology / Q000528:radiation effects; D005720:Gamma Rays; D020450:Heavy Ions; D006801:Humans; D018499:Linear Energy Transfer; D016296:Mutagenesis; D009154:Mutation / Q000528:radiation effects*; D009356:Neon; D012062:Relative Biological Effectiveness
D002588:Cesium Radioisotopes; D009356:Neon; D002244:Carbon