[Immunodetections and analysis of peripheral blood for patients with oral cancer undergoing operation].
OBJECTIVE
To investigate the immunological function for patients with oral cancer undergoing operation.


METHODS
Peripheral blood was taken from patients with oral cancer before and after operation. Proportions of CD3(+) total T, CD4(+) Th, CD8(+) Tc/Ts, CD19(+) total B, CD16(+)/CD56(+)NK were detected by flow cytometry (FCM). Detections of serum tumor necrosis factor alpha (TNF-alpha) and soluble interleukin-2 receptor (sIL-2R) were performed with enzyme-linked immunosorbent assay(ELISA). Paired t test was used for statistical analysis.


RESULTS
The proportions of CD8(+)Tc/Ts, CD19(+) total B and CD4(+)/CD8(+) increased significantly after surgery. Post operative proportion of CD16(+)/CD56(+)NK decreased more significantly. The concentration of TNF-alpha was less than that before operation(P<0.05).


CONCLUSIONS
Surgery could change immunological function and alleviate immunosuppression of oral cancer patients. Detections for immune conditions of oral cancer patients might have important clinical values in prognosis evaluation and guiding treatment, especially immunotherapy, after operation.
Ye
Dong-xia
DX
Department of Oral Immunology, School of Stomatology, Shanghai Second Medical University. Shanghai 200011,China.
Xu
Qin
Q
Chen
Wan-tao
WT
Zhu
Han-guang
HG
Lin
Guo-chu
GC
Jiang
Can-hua
CH
Qiu
Wei-liu
WL
D016516:CD4-CD8 Ratio; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D009062:Mouth Neoplasms / Q000276:immunology* / Q000601:surgery*; D015375:Receptors, Interleukin-2 / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D015375:Receptors, Interleukin-2; D014409:Tumor Necrosis Factor-alpha