title: Sensitization of recombinant vanilloid receptor-1 by various neurotrophic factors.
abstract: The vanilloid receptor (VR1) is a central integrator molecule of nociceptive stimuli. In this study, we have measured the effects of various neurotrophins (nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3, and -4) on recombinant rat VR1-mediated intracellular calcium rise in response to capsaicin in VR1/C6 cells. Our results clearly show that all neurotrophins sensitize the VR1 to capsaicin. Furthermore, using K252a, an inhibitor of tyrosine kinases, we present that actions of neurotrophins are mediated by the trk (A, B, C) receptors expressed in these cells. These data argue for the putative roles of neurotrophins in inducing inflammatory (thermal) hyperalgesia via VR1.
authors.affiliation: Department of Physiology and Cell Physiology Research Group of the Hungarian Academy of Sciences, H-4012 Debrecen, Nagyerdei krt. 98. PO Box 22, Hungary.
authors.forename: József
authors.initials: J
authors.lastname: Lázár
authors.forename: Tamás
authors.initials: T
authors.lastname: Szabó
authors.forename: Rita
authors.initials: R
authors.lastname: Marincsák
authors.forename: László
authors.initials: L
authors.lastname: Kovács
authors.forename: Peter M
authors.initials: PM
authors.lastname: Blumberg
authors.forename: Tamás
authors.initials: T
authors.lastname: Bíró
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism*; D002211:Capsaicin / Q000378:metabolism*; D006930:Hyperalgesia / Q000139:chemically induced; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009619:Nociceptors / Q000187:drug effects*; D051381:Rats; D011955:Receptors, Drug / Q000187:drug effects*; D011994:Recombinant Proteins / Q000187:drug effects; D050916:TRPV Cation Channels; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D009414:Nerve Growth Factors; D011955:Receptors, Drug; D011994:Recombinant Proteins; D050916:TRPV Cation Channels; C494484:TRPV1 receptor; D002211:Capsaicin; D002118:Calcium