title: A fragmentation study of a flavone triglycoside, kaempferol-3-O-robinoside-7-O-rhamnoside.
abstract: A mass spectrometric method based on the combined use of electrospray ionization, collision-induced dissociation and tandem mass spectrometry has been applied to the structural characterization of the flavone triglycoside, robinin (3,5,7,4'-tetrahydroxyflavone-3-O-robinoside-7-O-rhamnoside). The deprotonated molecule fragments by loss of the rhamnose glycan residue to yield the Y(7) (-) ion (m/z 593) and by scission of the robinose glycan residue to yield the radical anion [Y(3,0)-H](-.) (m/z 430). The Y(7) (-) ion fragments by scission of the robinose glycan residue to yield the radical anion of Y(7)[Y(3,0)-H](-.) (m/z 284). The [Y(3,0)-H](-.) radical anion fragments by loss of the rhamnose glycan residue to yield the radical anion Y(7)[Y(3,0)-H](-.) (m/z 284) and by scission to yield [Y(7)-H][Y(3,0)--H](-) (m/z 283). A fragmentation mechanism has been proposed.
authors.affiliation: Water Quality Centre, Trent University, Peterborough, Ontario K9J 7B8, Canada. rmarch@trentu.ca
authors.forename: Raymond E
authors.initials: RE
authors.lastname: March
authors.forename: Xiu-Sheng
authors.initials: XS
authors.lastname: Miao
authors.forename: Chris D
authors.initials: CD
authors.lastname: Metcalfe
mesh_terms: D005419:Flavonoids / Q000737:chemistry*; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D005419:Flavonoids; C005183:robinin