A new HPLC/UV method for the determination of clindamycin in dog blood serum.
A new HPLC method for the quantitative determination of clindamycin in dog blood serum at levels down to 80 ng/ml has been developed. Samples were deproteinised with acetonitrile and clindamycin was extracted with dichloromethane. Chromatographic analysis was carried out on a C(18) reversed-phase analytical column in the presence of tetra-n-butylammonium hydrogen sulfate (TBA), as an ion-pairing agent. UV detector wavelength was set at 195 nm. The assay was validated for a concentration range from 80 to 6000 ng/ml serum. Good linearity was observed in the entire concentration range. The limit of quantification (LOQ) was 80 ng/ml and the limit of detection (LOD) was 60 ng/ml. Regression of accuracy data yielded an overall mean recovery value (+/-S.E.M.) of 93.98+/-0.42%, while precision data revealed coefficient of variation (CV (%)) values lower than 4.41%. The method was successfully applied to determine drug concentrations in serum samples from dogs that had been orally administered clindamycin hydrochloride.
Batzias
G C
GC
Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Aristotle University of Thessaloniki, GR-54 124 Thessaloniki, Greece. batzias@vet.auth.gr
Delis
G A
GA
Koutsoviti-Papadopoulou
M
M
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000662:veterinary*; D002981:Clindamycin / Q000032:analysis / Q000097:blood*; D004283:Dog Diseases / Q000097:blood / Q000188:drug therapy; D004285:Dogs; D004355:Drug Stability; D013056:Spectrophotometry, Ultraviolet / Q000379:methods / Q000662:veterinary*; D013678:Technology, Pharmaceutical / Q000379:methods*
D002981:Clindamycin