Antimicrobial and antioxidative enrichment of oak (Quercus robur) bark by rotation planar extraction using ExtraChrom.
The multifunctional ExtraChrom instrument was used in the extraction of antimicrobial and radical scavenging components from oak (Quercus robur L.) bark. Milled and sieved oak bark was extracted with 80% (v/v) methanol solution in water on the ExtraChrom instrument using step-gradient in the preparative separation. Extracts were tested using agar diffusion method on Staphylococcus aureus, Enterobacter aerogenes and Candida albicans. Some extracts showed moderate bactericidal, fungicidal, bacteriostatic and fungistatic activity. The composition related to activity of the fractions and extracts was screened simultaneously by thin-layer chromatography (TLC) detected by UV and by spraying the plate with radical scavenging reagent 1,1-diphenyl-2-picrylhydrazyl (DPPH) to detect antioxidant activity. Thus, we could demonstrate the antiradical and antimicrobial activity of oak beneficial in the storage of wine against the oxidation and human microbial exposure.
Andrensek
Samo
S
Laboratory for Food Chemistry, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia.
Simonovska
Breda
B
Vovk
Irena
I
Fyhrquist
Pia
P
Vuorela
Heikki
H
Vuorela
Pia
P
D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development; D002855:Chromatography, Thin Layer; D015169:Colony Count, Microbial; D021902:Enterobacter aerogenes / Q000187:drug effects / Q000254:growth & development; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods*; D016166:Free Radical Scavengers / Q000494:pharmacology; D010084:Oxidation-Reduction; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification* / Q000494:pharmacology*; D029963:Quercus / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D014920:Wine
D016166:Free Radical Scavengers; D010936:Plant Extracts