The epiplasm gene EPC1 influences cell shape and cortical pattern in Tetrahymena thermophila.
The cortical protein Epc1p is the most abundant protein in the membrane skeleton, or epiplasm, of Tetrahymena thermophila. A partial sequence of the EPC1 gene was obtained and used to obtain a knockout construct that was successful in transforming Tetrahymena thermophila cells. The results support the conclusion that Epc1p influences cell shape and the fidelity of cortical development. It was further observed that this protein is transferred from plus to minus cells during conjugation, and that the imported protein is assembled into the epiplasm of the recipient cell in a discreet series of steps.
Williams
Norman E
NE
Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242, USA. norman-williams@uiowa.edu
D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D003227:Conjugation, Genetic; D003599:Cytoskeleton / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D017353:Gene Deletion; D017125:Genes, Protozoan; D007438:Introns; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames; D010641:Phenotype; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D016808:Tetrahymena thermophila / Q000166:cytology* / Q000235:genetics*; D014170:Transformation, Genetic
D020121:5' Untranslated Regions; D016054:DNA, Protozoan; D015800:Protozoan Proteins