[Extracellular carbohydrates and polysaccharides of the algae Chlorella pyrenoidosa Chick S-39].
In the growing culture of the thermophilic alga Chlorella pyrenoidosa Chick S-39, the amount of extracellular carbohydrates in the medium reached 5-17% of their content in the cells and 20-40% of the total content of extracellular organic matter. Experiments with the enrichment and synchronous algal cultures showed that the accumulation of extracellular carbohydrates and polysaccharides in the media occurred due to their release from the cells, rather than to cell lysis, and depended on cell photosynthetic activity and reproduction. Chromatographic determination of free sugars revealed the presence of saccharose, glucose, and fructose in the culture medium. Extracellular carbohydrates in C. pyrenoidosa cultures were represented mainly by water-soluble polysaccharides containing galactose, mannose, arabinose, xylose, ribose, fucose, and rhamnose.
Maksimova
I V
IV
Biological Faculty, Moscow State University, Vorob'evy gory, Moscow, 119892 Russia.
Bratkovskaia
L B
LB
Plekhanov
S E
SE
D001089:Arabinose / Q000032:analysis; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D049109:Cell Proliferation; D002478:Cells, Cultured; D002708:Chlorella / Q000254:growth & development / Q000378:metabolism*; D002854:Chromatography, Paper / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D005109:Extracellular Matrix / Q000378:metabolism*; D005643:Fucose / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D008358:Mannose / Q000032:analysis; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D014994:Xylose / Q000032:analysis
D002241:Carbohydrates; D003470:Culture Media; D011134:Polysaccharides; D005643:Fucose; D014994:Xylose; D001089:Arabinose; D005947:Glucose; D008358:Mannose; D005690:Galactose