Functionally deficient neuronal differentiation of mouse embryonic neural stem cells in vitro.
Embryonic mouse neural stem cells (NSCs) were isolated from E14 mice, multiplied in medium containing epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) and plated in laminin-coated wells in basic serum-free neurobasal medium. After 7 days in vitro, approximately 20% of the embryonic mouse NSCs developed into morphologically and biochemically fully maturated neurons, with extensive dendrites and multiple synaptic contacts. However, even after 22 days of culture, none of these neurons developed voltage-dependent sodium-channels characteristic for a functional neuron. Apparently, the morphological differentiation and the electrophysiological maturation of an embryonic mouse NSC into a neuron are independently regulated.
Balasubramaniyan
V
V
Department of Medical Physiology, University of Groningen, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands.
de Haas
A H
AH
Bakels
R
R
Koper
A
A
Boddeke
H W G M
HW
Copray
J C V M
JC
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D004558:Electric Stimulation / Q000379:methods; D004622:Embryo, Mammalian; D004815:Epidermal Growth Factor / Q000502:physiology; D016222:Fibroblast Growth Factor 2 / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000502:physiology; D007150:Immunohistochemistry / Q000379:methods; D008564:Membrane Potentials / Q000502:physiology / Q000528:radiation effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D018408:Patch-Clamp Techniques / Q000379:methods; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology
D005904:Glial Fibrillary Acidic Protein; D008869:Microtubule-Associated Proteins; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor