title: Electrochemically assisted co-precipitation of protein with calcium phosphate coatings on titanium alloy.
abstract: A bovine serum albumin protein-containing calcium phosphate coating (BSA/brushite) was prepared by electrochemically assisted co-precipitation onto a hydroxyapatite (HA) coated Ti-6Al-4V surface. Electrochemically assisted co-precipitation of BSA/brushite coatings onto HA resulted in a 70-fold increase in BSA inclusion compared to simple adsorption, and was subsequently released by a slower mechanism (15% loss over 70 h). Thus, this electrochemically assisted co-precipitation technique provides an efficient method of protein incorporation at physiological temperature, with a potential for sustained release of therapeutic agents as may be required for metallic implant fixation.
authors.affiliation: Department of Chemistry, Acadia University, 6 University Ave, Wolfville, Nova Scotia, Canada B4P 2R6.
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Cheng
authors.forename: Mark
authors.initials: M
authors.lastname: Filiaggi
authors.forename: Sharon G
authors.initials: SG
authors.lastname: Roscoe
mesh_terms: D000327:Adsorption; D000497:Alloys; D011232:Chemical Precipitation; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D008422:Materials Testing; D008968:Molecular Conformation; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000648:ultrastructure; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000648:ultrastructure*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D000497:Alloys; D020099:Coated Materials, Biocompatible; D011506:Proteins; C031462:titanium alloy (TiAl6V4); D012710:Serum Albumin, Bovine; D014025:Titanium