title: Dendritic cells from mice neonatally vaccinated with modified vaccinia virus Ankara transfer resistance against herpes simplex virus type I to naive one-week-old mice.
abstract: Modified vaccinia Ankara (MVA) is an attenuated virus. MVA induces the production of IFN and Flt3-L (FL), which results in the expansion of dendritic cells (DC) and enhanced resistance against viral infections. We report on the interplay among IFN, FL, and DC in the resistance against heterologous virus after injection of neonatal mice with MVA. The induction of serum FL was tested on day 2, and the expansion of DC was tested 1 wk after treatment with MVA. At this time point the resistance against infection with heterologous virus was also determined. After MVA treatment, serum FL was enhanced, and DC, including plasmacytoid cells in spleen, were increased in number. Mice that lacked functional IFN type I and II systems failed to increase both the concentration of FL and the number of DC. Treatment with MVA enhanced resistance against HSV-1 in wild-type animals 100-fold, but animals without a functional IFN system were not protected. Transfer of CD11c(+) cells from MVA-treated mice into naive animals protected against lethal infection with HSV-1. Thus, although the increased resistance could be largely attributed to the increase in activation of IFN-producing plasmacytoid cells, this, in turn, depends on a complex interplay between the DC and T cell systems involving both FL and IFNs.
authors.affiliation: Institute of Virology, University of Zurich, Zurich, Switzerland.
authors.forename: Marco
authors.initials: M
authors.lastname: Franchini
authors.forename: Hanspeter
authors.initials: H
authors.lastname: Hefti
authors.forename: Sabine
authors.initials: S
authors.lastname: Vollstedt
authors.forename: Bettina
authors.initials: B
authors.lastname: Glanzmann
authors.forename: Matthias
authors.initials: M
authors.lastname: Riesen
authors.forename: Mathias
authors.initials: M
authors.lastname: Ackermann
authors.forename: Paul
authors.initials: P
authors.lastname: Chaplin
authors.forename: Ken
authors.initials: K
authors.lastname: Shortman
authors.forename: Mark
authors.initials: M
authors.lastname: Suter
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D019264:Adoptive Transfer; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000276:immunology*; D016827:CD8 Antigens / Q000096:biosynthesis; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000637:transplantation*; D006561:Herpes Simplex / Q000276:immunology / Q000517:prevention & control*; D022301:Herpes Simplex Virus Vaccines / Q000008:administration & dosage / Q000276:immunology*; D018259:Herpesvirus 1, Human / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D007113:Immunity, Innate; D007370:Interferon Type I / Q000096:biosynthesis / Q000378:metabolism / Q000502:physiology; D008024:Ligands; D008565:Membrane Proteins / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D053633:Receptor, Interferon alpha-beta; D017471:Receptors, Interferon / Q000172:deficiency / Q000235:genetics; D013234:Stem Cells / Q000276:immunology / Q000378:metabolism; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology; D014616:Vaccinia virus / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D016827:CD8 Antigens; C431698:CD8alpha antigen; D022301:Herpes Simplex Virus Vaccines; D000949:Histocompatibility Antigens Class II; D007370:Interferon Type I; D008024:Ligands; D008565:Membrane Proteins; D017471:Receptors, Interferon; D014613:Vaccines, Attenuated; C084881:flt3 ligand protein; D053633:Receptor, Interferon alpha-beta