Enhanced motility of a Proteus mirabilis strain expressing hybrid FlaAB flagella.
Proteus mirabilis has two tandemly arranged flagellin-encoding genes, flaA and flaB. flaA is transcribed from a sigma(28) promoter, while flaB is silent. flaA and flaB can undergo reversible rearrangement to produce a set of hybrid genes referred to as flaAB. Flagellins composed of FlaAB protein have a different amino acid sequence and are antigenically distinct from flagellin composed of FlaA, implicating flagellin gene conversion as a putative virulence mechanism for P. mirabilis. The change in amino acid sequence is also hypothesized to alter the filament helix and, hence, affect the motility of FlaAB-expressing strains. To test this hypothesis, the motility of wild-type P. mirabilis was compared with that of a strain, DF1003, locked into the FlaAB(+) hybrid phase, under conditions of altered ionic strength, pH and viscosity. Cell motion tracking analysis showed that DF1003 has wild-type swimming velocity at physiological conditions, but moves significantly faster and travels further compared to the wild-type at NaCl concentrations greater than 170 mM. DF1003 is also significantly faster than the wild-type at pH 5.2, 5.8 and 8.2, and at 5 and 10 % polyvinylpyrrolidone. Measurements of amplitude and wavelength for isolated flagella subjected to pH 5.8 or 425 mM NaCl showed a loss of helical structure in FlaA flagella compared to FlaAB filaments, a feature that could significantly affect motility under these conditions. These results support a hypothesis that FlaAB flagellin imparts a motile advantage to P. mirabilis in conditions that otherwise may impede bacterial movement. In a broader context, flagellar antigenic variation, commonly thought to serve as means to avoid host defences, may also enhance motility in other bacterial species, thus aiding in the adaptation and survival of the cells.
Manos
Jim
J
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 710 East Pratt Street, Baltimore, MD 21202, USA.
Artimovich
Elena
E
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 710 East Pratt Street, Baltimore, MD 21202, USA.
Belas
Robert
R
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 710 East Pratt Street, Baltimore, MD 21202, USA.
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D003470:Culture Media; D005407:Flagella / Q000378:metabolism* / Q000648:ultrastructure; D005408:Flagellin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009068:Movement; D011513:Proteus mirabilis / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*; D011995:Recombination, Genetic; D012965:Sodium Chloride / Q000494:pharmacology
D003470:Culture Media; D005408:Flagellin; C067138:flaA protein, bacteria; C416532:flaB flagellin; D012965:Sodium Chloride