[Immunopotency of the recombinant urease B subunit vaccine of Helicobacter pylori after intranasal administration to mice].
OBJECTIVE
To observe the immunopotency of the recombinant urease B subunit (rUreB) of Helicobacter pylori after intranasal administration to mice. MDTHODS: BALB/c mice were immunized intranasally with rUreB of 20 microg,10 microg and rUreB plus different adjuvants, such as cholera toxin B subunit (CTB), Escherichia coli heat labile enterotoxin B subunit (LTB) and carbopol respectively, four times at an intervals of 7 days. The serum and washing solution from gastric, intestinal, nasal and tracheal mucosas were collected in 7 days after final immunization. IgG and IgA antibodies specific for rUreB were detected by ELISA.


RESULTS
The levels of IgA and IgG antibodies in sera every groups of mice immunized intranasally were significantly increased compared with control group (P<0.01). Only the levels of serum IgG of mice immunized with 20 microg dose were higher than those of mice immunized with 10 microg dose. Carbopol could enhance the level of IgA antibodies in washing solution from mouse gastric mucosa after intranasal immunization. The efficacy of LTB as a nasal mucosal immuno-adjuvant was stronger than that of CTB.


CONCLUSIONS
CTB, LTB and carbopol can play the role of adjuvant in nasal mucosal immunization. Intranasal immunization with rUreB can induce not only serum IgG antibody production but also antibody responses of different mucosa. Thus intranasal inoculation is a convenient, effective and cheap immunization way.
Gao
Zhi-gang
ZG
Department of Clinical Microbiology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, China.
Zou
Quan-ming
QM
Guo
Gang
G
Guo
Tong-sheng
TS
Zou
Quan-ming
QM
Zeng
Wei-kun
WK
Xie
Qing-hua
QH
D000180:Acrylic Resins; D000276:Adjuvants, Immunologic; D000281:Administration, Intranasal; D000818:Animals; D001427:Bacterial Toxins / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D002772:Cholera Toxin / Q000276:immunology; D004768:Enterotoxins / Q000276:immunology; D029968:Escherichia coli Proteins / Q000276:immunology; D005260:Female; D005753:Gastric Mucosa / Q000276:immunology; D016480:Helicobacter pylori / Q000276:immunology*; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000378:metabolism*; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000097:blood; D007413:Intestinal Mucosa / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009297:Nasal Mucosa / Q000276:immunology; D011145:Polyvinyls / Q000494:pharmacology; D011994:Recombinant Proteins / Q000276:immunology; D014510:Urease / Q000276:immunology*; D014611:Vaccination
D000180:Acrylic Resins; D000276:Adjuvants, Immunologic; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011145:Polyvinyls; D011994:Recombinant Proteins; C006912:carboxypolymethylene; D002772:Cholera Toxin; C027538:heat-labile enterotoxin, E coli; D014510:Urease