title: Expression profile analysis of wild-type and fcc1 mutant strains of Fusarium verticillioides during fumonisin biosynthesis.
abstract: Fusarium verticillioides produces a group of mycotoxins known as fumonisins that are associated with a variety of mycotoxicoses in humans and animals. In this study, DNA microarrays were constructed with expressed sequence tags (ESTs) from F. verticillioides. To identify genes with patterns of expression similar to the fumonisin biosynthetic (FUM) genes, the microarray was probed with labeled cDNAs originating from a wild-type strain and a fcc1 mutant grown on maize and in a defined medium adjusted to either pH 3 or pH 8. The comparative analyses revealed differential expression of genes corresponding to 116 ESTs when the fungal strains were grown on maize. Under different pH conditions, 166 ESTs were differentially expressed, and 19 ESTs were identified that displayed expression patterns similar to the FUM ESTs. These results provide candidate genes with potential roles in fumonisin biosynthesis.
authors.affiliation: Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Pirttilä
authors.forename: L M
authors.initials: LM
authors.lastname: McIntyre
authors.forename: G A
authors.initials: GA
authors.lastname: Payne
authors.forename: C P
authors.initials: CP
authors.lastname: Woloshuk
mesh_terms: D020224:Expressed Sequence Tags; D037341:Fumonisins / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005670:Fusarium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D003313:Zea mays / Q000382:microbiology
chemical_list: D037341:Fumonisins; D005656:Fungal Proteins