Fish as bioreactors: transgene expression of human coagulation factor VII in fish embryos.
A plasmid containing human coagulation factor VII (hFVII) complementary DNA regulated by a cytomegalovirus promoter was microinjected into fertilized eggs of zebrafish, African catfish, and tilapia. The active form of hFVll was detected in the fish embryos by various assays. This positive expression of human therapeutic protein in fish embryos demonstrates the possibility of exploitation of transgenic fish as bioreactors.
Hwang
Gyulin
G
Division of Cell Sciences, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, SO16 7PX, UK.
Müller
Ferenc
F
Rahman
M Aziz
MA
Williams
Darren W
DW
Murdock
Paul J
PJ
Pasi
K John
KJ
Goldspink
Geoffrey
G
Farahmand
Hamid
H
Maclean
Norman
N
D000818:Animals; D019149:Bioreactors; D001777:Blood Coagulation / Q000187:drug effects; D003587:Cytomegalovirus / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005167:Factor VII / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D005399:Fishes / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D008845:Microinjections; D010783:Photometry; D011401:Promoter Regions, Genetic / Q000235:genetics; D019076:Transgenes / Q000235:genetics*; D015053:Zygote / Q000378:metabolism*
D018076:DNA, Complementary; D005167:Factor VII