title: Upregulation of heparin-binding growth-associated molecule after spinal cord injury in adult rats.
abstract: OBJECTIVE
  To investigate whether traumatic spinal cord injury (SCI) induces changes of gene expression of heparin-binding growth-associated molecule (HB-GAM).
  
  
  METHODS
  In a spinal cord transection model, HB-GAM expression and cellular localization were examined using Northern blot, RT-PCR, immunohistochemistry and immunofluorescence double-labeling methods.
  
  
  RESULTS
  HB-GAM mRNA was significantly upregulated in spinal cord tissues rostral and caudal to the injury at 7 d after SCI. HB-GAM gene expression was markedly increased at 3 d, peaked at 7 d, and declined to the baseline level at 28 d post-injury. During its peak expression, HB-GAM was co-localized in astrocytes, oligodendrocytes, and neurons in spinal cord tissues within 7 mm from the site of spinal transection.
  
  
  CONCLUSIONS
  SCI induces HB-GAM expression at both mRNA and protein levels in areas close to the injury. Both neurons and glial cells expressed HB-GAM implying that HB-GAM played a role in the process of injury and/or repair following SCI.
authors.affiliation: Department of Neurobiology, Shanghai Second Medical University, Shanghai 200025, China.
authors.forename: Yan-Ting
authors.initials: YT
authors.lastname: Wang
authors.forename: Shu
authors.initials: S
authors.lastname: Han
authors.forename: Kai-Hua
authors.initials: KH
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Jin
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Xu
authors.forename: Pei-Hua
authors.initials: PH
authors.lastname: Lu
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*; D013119:Spinal Cord Injuries / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D002352:Carrier Proteins; D016207:Cytokines; D012333:RNA, Messenger; C066845:pleiotrophin