A novel hydrophilic support, CoFoam, for enzyme immobilization.
Lipases from different sources (porcine pancreas, Mucor miehei and Candida antarctica B) were covalently immobilized on a hydrophilic polyurethane composite (CoFoam). Their hydrolytic activities assayed with tributyrin were 0.55, 2.1 and 447 U g(-1), respectively. The activity of the C. antarctica B composite in the synthesis of methyl oleate in hexane was 8.8 U g(-1) compared to 60.6 U g(-1) for commercial Novozyme 435. The advantage of the CoFoam composite lies in the low pressure drop in a packed-bed reactor at fairly large flow rates. For example, at flow rates of 10-12 l min(-1), the pressure drop over 15 cm is typically 3 kPa.
Vasudevan
P T
PT
Department of Chemical Engineering, University of New Hampshire, Durham, NH 03824, USA.
López-Cortés
N
N
Caswell
H
H
Reyes-Duarte
D
D
Plou
F J
FJ
Ballesteros
A
A
Como
K
K
Thomson
T
T
D000818:Animals; D019149:Bioreactors; D002175:Candida / Q000737:chemistry; D002384:Catalysis; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D007700:Kinetics; D008049:Lipase / Q000737:chemistry*; D009089:Mucor / Q000737:chemistry; D010179:Pancreas / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry*; D011312:Pressure; D013379:Substrate Specificity; D013552:Swine
D004800:Enzymes, Immobilized; D011140:Polyurethanes; D008049:Lipase