title: Biochemical effect evaluation of perfluorooctane sulfonic acid-contaminated wood mice (Apodemus sylvaticus).
abstract: Wood mice (Apodemus sylvaticus) were captured at Blokkersdijk, a nature reserve in the immediate vicinity of a fluorochemical plant in Antwerp, Belgium, and at Galgenweel, 3 kilometers farther away. The liver perfluorooctane sulfonic acid (PFOS) concentrations in the Blokkersdijk mice were extremely high (0.47-178.55 micro g/g wet weight). Perfluorononanoic, perfluorodecanoic, perfluoroundecanoic, and perfluorododecanoic acids were found sporadically in the liver tissue of the Blokkersdijk mice. The liver PFOS concentrations at Galgenweel were significantly lower than those at Blokkersdijk (0.14-1.11 micro g/g wet weight). Further results suggest sex independence of the liver PFOS levels, increased levels of PFOS bioaccumulation in older mice, and maternal PFOS transfer to the young. Several liver end points were significantly elevated in the Blokkersdijk mice: liver weight, relative liver weight, peroxisomal beta-oxidation activity, microsomal lipid peroxidation level, and mitochondrial fraction protein content. For the mitochondrial fraction catalase activity, no significant difference between locations was found. The liver weight, relative liver weight, and liver microsomal lipid peroxidation level increased significantly with the liver PFOS concentration. No indications for PFOS-mediated effects on the serum triglyceride, cholesterol, or potassium levels were obtained. The liver PFOS concentration was negatively related to the serum alanine aminotransferase activity.
authors.affiliation: Department of Biology, Research Unit Ecophysiology, Biochemistry and Toxicology, Antwerp University, Antwerp, Belgium. phoff@ruca.ua.ac.be
authors.forename: Philippe Tony
authors.initials: PT
authors.lastname: Hoff
authors.forename: Jan
authors.initials: J
authors.lastname: Scheirs
authors.forename: Kristin
authors.initials: K
authors.lastname: Van de Vijver
authors.forename: Walter
authors.initials: W
authors.lastname: Van Dongen
authors.forename: Eddy Louis
authors.initials: EL
authors.lastname: Esmans
authors.forename: Ronny
authors.initials: R
authors.lastname: Blust
authors.forename: Wim
authors.initials: W
authors.lastname: De Coen
mesh_terms: D000410:Alanine Transaminase / Q000494:pharmacology; D017738:Alkanesulfonic Acids / Q000493:pharmacokinetics* / Q000633:toxicity*; D000818:Animals; D004785:Environmental Pollutants / Q000493:pharmacokinetics* / Q000633:toxicity*; D005227:Fatty Acids / Q000493:pharmacokinetics* / Q000633:toxicity*; D005260:Female; D005466:Fluorocarbons / Q000493:pharmacokinetics* / Q000633:toxicity*; D015227:Lipid Peroxidation; D008099:Liver / Q000737:chemistry* / Q000201:enzymology; D008297:Male; D008928:Mitochondria / Q000502:physiology; D009115:Muridae; D012737:Sex Factors; D014018:Tissue Distribution
chemical_list: D017738:Alkanesulfonic Acids; D004785:Environmental Pollutants; D005227:Fatty Acids; D005466:Fluorocarbons; C586085:perfluoroundecanoic acid; C076994:perfluorooctane sulfonic acid; D000410:Alanine Transaminase