title: [Competitive immunochemical determination of antigens using conjugates containing Co(II) and Ni(II)].
abstract: A new variant of competitive heterogeneous immunoassay for certain proteinaceous antigens has been developed. The assay is based on the use of the target protein conjugated with Co(II) or Ni(II) ions and immobilized Abs. The effect of catalytic hydrogen release allows quantitation of the metal ion labels by voltammetry at the final step of the assay. The conjugates have been characterized by spectrophotometry, voltammetry, atomic adsorption spectrometry, and nuclear magnetic relaxation. Based on the use of the conjugate RNase-diethylenetriaminepentaacetic acid-Co(II) (10:4:4), a competitive immunoassay for RNase has been developed, detecting the target protein in the range 2 x 10(-2)-2 x 10(-4) mg/ml.
authors.affiliation: Faculty of Chemistry, Kazan State University, Kazan, 420008 Russia.
authors.forename: Iu I
authors.initials: IuI
authors.lastname: Dykhal
authors.forename: E P
authors.initials: EP
authors.lastname: Mediantseva
authors.forename: N R
authors.initials: NR
authors.lastname: Murtazina
authors.forename: N V
authors.initials: NV
authors.lastname: Kalacheva
authors.forename: G K
authors.initials: GK
authors.lastname: Budnikov
mesh_terms: D000941:Antigens / Q000032:analysis*; D002384:Catalysis; D003035:Cobalt / Q000032:analysis; D007120:Immunochemistry / Q000379:methods; D009682:Magnetic Resonance Spectroscopy; D009532:Nickel / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012260:Ribonucleases / Q000032:analysis*; D013054:Spectrophotometry, Atomic
chemical_list: D000941:Antigens; D011506:Proteins; D003035:Cobalt; D009532:Nickel; D012260:Ribonucleases