title: Matrix metalloproteinase-1 polymorphism of promoter region in sarcoidosis and tuberculosis patients.
abstract: OBJECTIVE
  Matrix metalloproteinases (MMPs) are associated with several diseases. MMP-1 is one of the interstitial collagenases and the most highly expressed. Recent studies have found that a single nucleotide polymorphism located in the promoter region of the MMP-1 gene affects transcriptional activity. This polymorphism, 1G/2G, has been reported to associate with several malignant tumors and lung diseases. In this study, we investigated whether this polymorphism is associated with sarcoidosis or tuberculosis.
  
  
  METHODS
  Polymerase chain reaction-restriction fragment length polymorphism was used to determine the MMP-1 genotypes of 103 sarcoidosis patients, 105 tuberculosis patients and 106 healthy control subjects.
  
  
  RESULTS
  We found no differences in genotype distributions and allele frequency between sarcoidosis or tuberculosis patients and healthy control subjects. In sarcoidosis patients with ocular involvement, a significant increase in 1G/1G or 1G/2G genotype was observed compared with patients without (p = 0.009, odds ratio (OR) = 3.22, 95% confidence interval (CI): 1.34-7.71). In sarcoidosis patients with three or more organs involved, 1G/1G type tended to increase compared with patients without (p = 0.035, OR = 5.17, 95% CI: 1.12-23.9). In tuberculosis patients with cavity formation, an increasing trend of 1G/1G type was observed compared with patients without (p = 0.064, OR = 7.69, 95% CI: 0.89-66.3).
  
  
  CONCLUSIONS
  Although MMP-1 polymorphism was not associated with onset risk of sarcoidosis and tuberculosis, the clinical characteristics of both diseases were affected by this polymorphism.
authors.affiliation: Department of Internal Medicine and Molecular Science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Aichi, Japan.
authors.forename: Shigemitsu
authors.initials: S
authors.lastname: Ninomiya
authors.forename: Takashi
authors.initials: T
authors.lastname: Niimi
authors.forename: Shigeki
authors.initials: S
authors.lastname: Shimizu
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sato
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Achiwa
authors.forename: Hidemi
authors.initials: H
authors.lastname: Ito
authors.forename: Kenji
authors.initials: K
authors.lastname: Akita
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Maeda
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Ueda
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005128:Eye Diseases / Q000473:pathology; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic / Q000235:genetics; D012307:Risk Factors; D012507:Sarcoidosis / Q000235:genetics*; D014397:Tuberculosis, Pulmonary / Q000235:genetics*
chemical_list: D020781:Matrix Metalloproteinase 1