Tuning the redox and enzymatic activity of glucose oxidase in layered organic films and its application in glucose biosensors.
Glucose oxidase was embedded in organic films through a layer-by-layer approach, where the enzyme demonstrated significantly enhanced electron-transfer reactivity and finely tuned enzymatic activity. An unmediated, reagentless glucose biosensor was accordingly prepared with two polyethylenimine/glucose oxidase bilayers-modified pyrolytic graphite electrode. A calibration linear range of glucose was 0.5-8.9 mM with a detection limit of 50 microM and sensitivity of 0.76 microA mM(-1).
Zhang
Wenjun
W
Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, People's Republic of China.
Huang
Yinxi
Y
Dai
Han
H
Wang
Xiaoyue
X
Fan
Chunhai
C
Li
Genxi
G
D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000295:instrumentation*; D002384:Catalysis; D004563:Electrochemistry; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity
D005949:Glucose Oxidase; D005947:Glucose