title: The first fluorescent diboronic acid sensor specific for hepatocellular carcinoma cells expressing sialyl Lewis X.
abstract: Carbohydrate antigens with subterminal fucosylation have been implicated in the development and progression of several cancers, including hepatocellular carcinoma (HCC). Fluorescent sensors targeting fucosylated carbohydrate antigens could potentially be used for diagnostic and other applications. We have designed and synthesized a series of 26 diboronic acid compounds as potential fluorescent sensors for such carbohydrates. Among these compounds, 7q was able to fluorescently label cells expressing high levels of sLex (HEPG2) within a concentration range of 0.5 to 10 microM. This compound (7q) did not label cells expressing Lewis Y (HEP3B), nor cells without fucosylated antigens (COS7). This represents the first example of a fluorescent compound labeling cells based on cell surface carbohydrate structures.
authors.affiliation: Department of Chemistry, Georgia State University, 33 Gilmer Street S.E., Atlanta, GA 30303, USA.
authors.forename: Wenqian
authors.initials: W
authors.lastname: Yang
authors.forename: Haiying
authors.initials: H
authors.lastname: Fan
authors.forename: Xingming
authors.initials: X
authors.lastname: Gao
authors.forename: Shouhai
authors.initials: S
authors.lastname: Gao
authors.forename: Vishnu Vardhan Reddy
authors.initials: VV
authors.lastname: Karnati
authors.forename: Weijuan
authors.initials: W
authors.lastname: Ni
authors.forename: W Borden
authors.initials: WB
authors.lastname: Hooks
authors.forename: John
authors.initials: J
authors.lastname: Carson
authors.forename: Brent
authors.initials: B
authors.lastname: Weston
authors.forename: Binghe
authors.initials: B
authors.lastname: Wang
mesh_terms: D000818:Animals; D001897:Boronic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line; D005453:Fluorescence; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015394:Molecular Structure; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D000080506:Sialyl Lewis X Antigen
chemical_list: D001897:Boronic Acids; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen