title: Effect of red and blue light on the timing of cyclin-dependent kinase activity and the timing of cell division in Chlamydomonas reinhardtii.
abstract: In this study, we describe the effect of red and blue light on the timing of cell division, DNA synthesis, and activity and presence of cyclin-dependent kinases (CDKs), in synchronous cultures of the unicellular green alga Chlamydomonas reinhardtii. Cell division and DNA synthesis were found to occur later in cells grown in blue or white light, than in red light. CDK-like activity, measured using a histone H1 kinase assay, correspondingly occurred later in cultures that were grown in blue light compared to cultures grown in red light. The amount of CDK-like proteins, as detected using an antibody against the PSTAIRE motif, showed a maximum during the division phase. We conclude that the mechanism that causes the delay in the timing of cell division in blue light has its action before DNA replication takes place and also precedes the increase in CDK-like activity.
authors.affiliation: Laboratory of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands. jet_oldenhof@yahoo.com
authors.forename: Harriëtte
authors.initials: H
authors.lastname: Oldenhof
authors.forename: Katerina
authors.initials: K
authors.lastname: Bisová
authors.forename: Herman
authors.initials: H
authors.lastname: van den Ende
authors.forename: Vilém
authors.initials: V
authors.lastname: Zachleder
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D015153:Blotting, Western; D002455:Cell Division / Q000502:physiology / Q000528:radiation effects; D002478:Cells, Cultured; D016825:Chlamydomonas reinhardtii / Q000166:cytology / Q000201:enzymology* / Q000528:radiation effects*; D018844:Cyclin-Dependent Kinases / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects*; D004247:DNA / Q000096:biosynthesis; D008027:Light; D011494:Protein Kinases / Q000378:metabolism; D013997:Time Factors
chemical_list: D004247:DNA; D011494:Protein Kinases; C102333:histone H1 kinase; D018844:Cyclin-Dependent Kinases