Immunohistochemistry for the detection of swine hepatitis E virus in the liver.
Swine hepatitis E virus (HEV) antigen was detected immunohistochemically in formalin-fixed, paraffin-embedded hepatic tissue from 30 naturally infected pigs. Thirty pigs from 30 different herds were selected on the basis of positive results for reverse transcription-polymerase chain reaction. Positive cells typically exhibited a red reaction product in the cytoplasm without any observable background staining. Swine HEV antigen was consistently detected in liver from all 30 pigs tested. A strong immunohistochemical signal was seen within a variable number of hepatocytes in multifocal lobules. The signal involved the majority of hepatocytes diffusely or was confined to foci of liver cells. Positive immunohistochemical signals were also detected in small and large intestine, lymph node, tonsil, spleen, and kidney. The immunohistochemistry technique developed in this study proved useful for the detection of swine HEV in formalin-fixed, paraffin-embedded tissues taken from naturally infected pigs and may be a valuable tool in studying the pathogenesis of swine HEV infection.
Ha
S-K
SK
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
Chae
C
C
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis / Q000235:genetics; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D016752:Hepatitis E virus / Q000235:genetics / Q000302:isolation & purification*; D006524:Hepatitis, Viral, Animal / Q000821:virology*; D007150:Immunohistochemistry / Q000379:methods; D008099:Liver / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D034421:Sus scrofa / Q000821:virology*; D014773:Virology / Q000379:methods
D000956:Antigens, Viral; D004279:DNA, Viral