title: Sequencing of two new HLA class II alleles: DRB3*0218 and DQB1*030202.
abstract: Two novel human leukocyte antigen (HLA) class II alleles for DRB3 and DQB1 genes detected in Caucasoid Spanish individuals are described: DRB3*0218 and DQB1*030202. Both alleles have been found during routine high-resolution typing by sequencing. DRB3*0218 shows a novel DRB3 gene polymorphic position, located at amino acid residue 58, alanine to glutamic acid. This residue is shared by several DRB1 alleles, including all described DRB1*11 subtypes. DQB1*030202 differs from DQB1*030201 by a point mutation at position 319 (T to C). This nucleotide change generates a new codon at amino acid position 75 that is not shared by any other DQB1 allele.
authors.affiliation: Histocompatibility Department, Regional Transfusion Center of Madrid, Madrid, Spain. abalas.trans@salud.madrid.org
authors.forename: A
authors.initials: A
authors.lastname: Balas
authors.forename: M J
authors.initials: MJ
authors.lastname: Aviles
authors.forename: R
authors.initials: R
authors.lastname: Lillo
authors.forename: M
authors.initials: M
authors.lastname: Alonso-Nieto
authors.forename: L
authors.initials: L
authors.lastname: Zarapuz
authors.forename: R
authors.initials: R
authors.lastname: García-Villaescusa
authors.forename: F
authors.initials: F
authors.lastname: García-Sánchez
authors.forename: J L
authors.initials: JL
authors.lastname: Vicario
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D005091:Exons; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics*; D059826:HLA-DRB3 Chains; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment
chemical_list: D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059826:HLA-DRB3 Chains; C495082:HLA-DRB3*02:18 antigen