title: Validity of plasma remnant lipoproteins as surrogate markers of antemortem level in cases of sudden coronary death.
abstract: Hitherto triglycerides (TG) and TG-rich lipoproteins were been of limited value as surrogates for antemortem levels. We measured TG levels in postmortem plasma from sudden coronary death cases (SCD, n=91) by using two TG assays, Dry Chem TG (free glycerol was added) and the Determiner L-TG (without added free glycerol) that measured net TG. TG levels were markedly higher by the Dry Chem TG (y) vs. Determiner L-TG (x), y = 1.03x + 229 mg/dl. HPLC showed large amounts of free glycerol in postmortem plasma and in TG-rich lipoprotein remnants (RLP). These results were verified in a rabbit model of SCD. Further, RLP from SCD were found to be biophysically similar to those from living patients with coronary artery disease (CAD). In conclusion, postmortem plasma sampled up to 12 h after death is appropriate for measuring lipid and lipoproteins, TG and RLP-TG as surrogates for antemortem levels when a TG assay without added free glycerol is used.
authors.affiliation: Department of Forensic Medicine, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan. takeichi@is.icc.u-tokai.ac.jp
authors.forename: Sanae
authors.initials: S
authors.lastname: Takeichi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Nakajima
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Yukawa
authors.forename: Masaki Q
authors.initials: MQ
authors.lastname: Fujita
authors.forename: Takeshi
authors.initials: T
authors.lastname: Saito
authors.forename: Fumiko
authors.initials: F
authors.lastname: Satoh
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Seto
authors.forename: Takahiko
authors.initials: T
authors.lastname: Kusakabe
authors.forename: Zheng-Bin
authors.initials: ZB
authors.lastname: Jin
authors.forename: Iwao
authors.initials: I
authors.lastname: Hasegawa
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Nakano
authors.forename: Abby
authors.initials: A
authors.lastname: Saniabadi
authors.forename: Masakazu
authors.initials: M
authors.lastname: Adachi
authors.forename: Naoki
authors.initials: N
authors.lastname: Ohara
authors.forename: Shinichi
authors.initials: S
authors.lastname: Usui
authors.forename: Mitsuyo
authors.initials: M
authors.lastname: Okazaki
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Nakajima
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D015415:Biomarkers / Q000097:blood; D002784:Cholesterol / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D016757:Death, Sudden, Cardiac; D005260:Female; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011180:Postmortem Changes; D011817:Rabbits; D015203:Reproducibility of Results; D014280:Triglycerides / Q000097:blood
chemical_list: D015415:Biomarkers; D008074:Lipoproteins; D014280:Triglycerides; D002784:Cholesterol