B- and T-cell markers in opsoclonus-myoclonus syndrome: immunophenotyping of CSF lymphocytes.
BACKGROUND
Although many lines of evidence suggest an autoimmune etiology, the pathophysiology of opsoclonus-myoclonus syndrome (OMS) remains poorly understood and no immunologic abnormalities have correlated with neurologic severity. Conventional immunotherapies often do not prevent relapse or permanent sequelae.


OBJECTIVE
To test the cellular immune hypothesis of OMS in a cross-sectional study and determine if CSF lymphocyte subset analysis provides biomarkers of disease activity.


METHODS
The expression of lymphocyte surface antigens was investigated in CSF and blood of 36 children with OMS and 18 control subjects, using a comprehensive panel of monoclonal antibodies to adhesion and activation proteins in combination with anti-CD3 and anti-CD45 antibodies in four-color fluorescence-activated cell sorting.


RESULTS
Although most children with OMS had normal CSF cell counts, they exhibited expansion of CD19+ B-cell (up to 29%) and gammadelta T-cell (up to 26%) subsets and a lower percentage of CD4+ T-cells and CD4/CD8 ratio, which persisted even years after disease onset and conventional treatments. The percentage of activated CSF T-cells was also higher. Abnormalities correlated with neurologic severity, as scored blinded from videotapes using a 12-item motor scale, and disease duration. No significant differences were found between tumor and no-tumor groups. In children with neuroblastoma, tumor resection or cancer chemotherapy did not alter immunologic abnormalities.


CONCLUSIONS
CSF B- and T-cell recruitment is linked to neurologic signs in pediatric OMS, which may relate to relapses and disease progression.
Pranzatelli
M R
MR
Department of Neurology, Southern Illinois University School of Medicine, Springfield, IL 62794-9658, USA. mpranzatelli@siumed.edu
Travelstead
A L
AL
Tate
E D
ED
Allison
T J
TJ
Moticka
E J
EJ
Franz
D N
DN
Nigro
M A
MA
Parke
J T
JT
Stumpf
D A
DA
Verhulst
S J
SJ
D018941:Antigens, CD19 / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D015415:Biomarkers / Q000134:cerebrospinal fluid*; D018450:Disease Progression; D006801:Humans; D016130:Immunophenotyping; D008213:Lymphocyte Activation; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000166:cytology / Q000276:immunology; D020361:Paraneoplastic Syndromes, Nervous System / Q000134:cerebrospinal fluid* / Q000276:immunology; D012008:Recurrence; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D018941:Antigens, CD19; D000954:Antigens, Surface; D015415:Biomarkers