Role of Ca2+ in induction of neurotransmitter-related gene expression by butyrate.
We examined the effect of butyrate on neurotransmitter-related gene expression and calcium homeostasis in PC12 cells. Pretreatment with Ca2+ chelators (EGTA or BAPTA-AM) attenuated the butyrate-triggered accumulation of TH and ppEnk mRNA indicating that Ca2+ plays a role in butyrate-induced regulation of neuronal genes. Butyrate alone did not alter intracellular Ca2+ levels as determined by Fura-PE3 fluorescence; however, pretreatment with butyrate (18-24 h) reduced the first Ca2+ peak and prevented the second sustained rise in [Ca2+]i as induced by nicotine or ryanodine. In contrast, butyrate had no effect on Ca2+ transients when added shortly before or during nicotine or ryanodine stimulation. These results suggest that chronic butyrate exposure can modulate cell responses by affecting intracellular Ca2+ signaling.
Zia
Muhammad T
MT
Division of Newborn Medicine Westchester Medical Center/Department of Pediatrics; Department of Biochemistry and Molecular Biology, The Regional Neonatal Center, New York Medical College-Westchester Medical Center, Valhalla, NY 10595, USA.
Nankova
Bistra B
BB
Krishnan
Kavitha
K
Gueorguiev
Volodia D
VD
Frenz
Christopher M
CM
Sabban
Esther L
EL
La Gamma
Edmund F
EF
D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D002118:Calcium / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000502:physiology; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D018377:Neurotransmitter Agents / Q000096:biosynthesis* / Q000378:metabolism; D016716:PC12 Cells; D051381:Rats
D002087:Butyrates; D018377:Neurotransmitter Agents; D002118:Calcium