Lentiviral and MLV based retroviral vectors for ex vivo and in vivo gene transfer.
Retroviral vectors have become an important tool for gene transfer in vitro and in vivo. Classical Moloney murine leukemia virus (MLV) based retroviral vectors have been used for over 20 years to transfer genes into dividing cells. Cell lines for production of retroviral vectors have become commonly available and modifications in retroviral vector design and use of envelope proteins have made the production of high titer, helper-free, infectious virus stocks relatively easy. More recently, lentiviral vectors, another class of retroviruses, have been modified for in vitro and in vivo gene transfer. The ability of lentiviral vectors to transduce non-dividing cells has made them especially attractive for in vivo gene transfer into differentiated, non-dividing tissues. Several improvements in helper plasmids and vectors have made lentivirus a safe vector system for ex vivo and in vivo gene transfer. This review will briefly summarize the background of these vector systems and provide some common protocols available for the preparation of MLV based retroviral vectors and HIV-1 based lentiviral vectors.
Blesch
Armin
A
Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093-0626, USA. ablesch@ucsd.edu
D000818:Animals; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics*; D014162:Transfection / Q000379:methods*