A complex prescription for vitiligo activates mitochondrial ATP synthase-6 expression in B-16 murine melanoma cells.
A complex prescription (CP) used traditionally for the treatment of vitiligo was prepared from water extract of eight plant materials. To study the mechanism of this preparation its effect on gene expression of B-16 murine melanoma cells was estimated using differential display method (DDRT-PCR). The results showed that the complex prescription was effective in activating the mitochondrial ATP synthase-6 (ATPase-6) gene expression in B-16 murine melanoma cells. This activity may play an important role in its treatment of vitiligo.
Li
Wenyong
W
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China.
Yu
Weidong
W
Dong
Qun
Q
Wang
Ping
P
Chen
Qingxuan
Q
D000818:Animals; D015152:Blotting, Northern; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D008546:Melanoma, Experimental / Q000473:pathology; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology*; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics*; D008954:Models, Biological; D010946:Plants, Medicinal; D012333:RNA, Messenger / Q000235:genetics; D014820:Vitiligo / Q000188:drug therapy* / Q000201:enzymology
D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; C412204:ATP synthase subunit 6; D025261:Mitochondrial Proton-Translocating ATPases