title: Human programmed cell death 5 protein has a helical-core and two dissociated structural regions.
abstract: Programmed cell death 5 (PDCD5) protein is phylogenetically conserved in both the nucleus and cytoplasm. The human PDCD5 protein is expressed in tumor cells during apoptosis independent of the apoptosis-inducing stimuli, and recently it was found that PDCD5 is an important regulator in both apoptotic and non-apoptotic programmed cell death. In this study, human PDCD5 was expressed in Escherichia coli cell and studied using heteronuclear NMR method. The NMR results indicate that PDCD5 protein can be divided into three structural regions, a core region and two dissociated terminal regions. The core region (41-101) represents a rigid sub-domain consisting mainly of a triple-helix bundle. The N-terminal 38 residues (3-40) are ordered, but not a rigid structural region which contains abundant secondary structure, and packs very loosely against the core. The C-terminal 17 residues (102-118) represent a mobile unstructured region, which may be capable of interaction with nucleic acid.
authors.affiliation: National Laboratory of Biomacromolecules, Center for Molecular Biology, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Liu
authors.forename: Yingang
authors.initials: Y
authors.lastname: Feng
authors.forename: Yuan
authors.initials: Y
authors.lastname: Cheng
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D051017:Apoptosis Regulatory Proteins; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D000596:Amino Acids; D051017:Apoptosis Regulatory Proteins; D009363:Neoplasm Proteins; C117283:PDCD5 protein, human; D011993:Recombinant Fusion Proteins