Rapid preparation of vaccinia virus DNA template for analysis and cloning by PCR.
This chapter describes the preparation of template DNA from poxvirus-infected cells, plaques, or crude virus stocks for polymerase chain reaction (PCR) amplification. The advantages of this technique are that it is rapid, inexpensive, and, most importantly, reliable, requiring only centrifugation, detergent, and protease treatment. The template preparation is suitable for PCR amplification for screening viruses, cloning, transfection, and DNA sequencing.
Roper
Rachel L
RL
Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC, USA.
D003001:Cloning, Molecular / Q000379:methods; D004277:DNA, Single-Stranded; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005821:Genetic Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D014616:Vaccinia virus / Q000235:genetics*
D004277:DNA, Single-Stranded; D004279:DNA, Viral