[Evaluation of the AOAC 985.29 enzimic gravimetric method for determination of dietary fiber in oat and corn grains].
The precision attributes and use of the enzymatic-gravimetric method of Prosky et al. (1992) (AOAC 985.29) were evaluated using corn (BR 5202 Pampa) and oat (UFRGS 15) samples. The effect of laboratory batches carried out in different days were evaluated in six laboratory batches, using for each material one duplicate for total fiber (FT) determination, one duplicate for insoluble fiber (FI) determination and blank ones for FT and for FI (both in duplicate). In order to characterize repetitive aspects, five other FT and FI determinations added to each sample were evaluated, summing up 11 data. The low coefficients of variation in the first six batches were considered acceptable as an expression of expected total intralaboratory variation. The repetitive of the method was considered good for FT determinations (CVs < 10%). However, in the FI determination a high frequency of negative values of ash and blanks was found, impairing the repetitive aspects evaluation. The magnitude of the total gravimetric corrections varies with the kind of the sample and is especially influenced by the protein content.
da Silva
Leila Picolli
LP
Centro de Ciências Rurais da Universidade Federal de Santa Maria, Universidade Federal do Rio Grande do Sul, Universidade do Rio Grande, Brasil.
Ciocca
Maria de Lourdes Santorio
Mde L
Furlong
Eliana Badiale
EB
D018554:Avena; D004043:Dietary Fiber / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D015203:Reproducibility of Results; D012995:Solubility; D003313:Zea mays