Selective precipitation and recovery of xylanase using surfactant and organic solvent.
The selective precipitation of xylanase from an aqueous phase containing mixtures of xylanase and cellulase was studied using an ionic surfactant as precipitating ligand and a polar organic solvent as recovery solvent. Of four ionic surfactants tested, sodium di-(2-ethylhexyl) sulfosuccinate (AOT) showed a complete removal of xylanase at pH 4.5. The recovery of xylanase from the xylanase-AOT complex was a strong function of the type and the volume of the polar solvent and of the concentration of sodium acetate buffer in the final aqueous solution used to solubilize the recovered xylanase. With ethanol as a recovery solvent, a recovery of xylanase activity of 78 +/- 10% was obtained. The cellulase activity in the recovered xylanase was below the detection limit. The results demonstrate that an ionic surfactant can recover enzymes from aqueous solutions without loss in their activity.
Shin
Youn-Ok
YO
Department of Chemical Engineering, McGill University, Montreal, Quebec, H3A 2B2 Canada.
Wahnon
Daphne
D
Weber
Martin E
ME
Vera
Juan H
JH
D002480:Cellulase / Q000737:chemistry; D011232:Chemical Precipitation; D004143:Dioctyl Sulfosuccinic Acid / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D000431:Ethanol / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D009930:Organic Chemicals / Q000737:chemistry*; D012996:Solutions; D012997:Solvents / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*; D014867:Water / Q000737:chemistry; D014995:Xylosidases / Q000737:chemistry* / Q000302:isolation & purification*
D008024:Ligands; D009930:Organic Chemicals; D012996:Solutions; D012997:Solvents; D013501:Surface-Active Agents; D014867:Water; D004143:Dioctyl Sulfosuccinic Acid; D000431:Ethanol; D014995:Xylosidases; D002480:Cellulase