title: [Isolation and purification of gonyautoxins from Alexandrium mimutum Halim].
abstract: OBJECTIVE
  To isolate and purify gonyautoxins from Alexandrium mimutum Halim Amtk2 strain.
  
  
  METHODS
  The ethanol extracts of culture Alexandriun minutum Halim Amtk2 were isolated by means of gel filtration chromatography, the toxin fraction obtained was then purified by ion exchange chromatography.
  
  
  RESULTS
  From 100 liter of cultivation liquid of Alexandrium mimutum Halim Amtk2 (6.74 +/- 0.31) x 10(9) cells were obtained. The ethanol extracts of Alexandriun minutum Halim purified by gel filtration chromatography obtained gonyautoxins mixture 29.59 mg. 4.06 mg of the mixture was further purified by two steps of ion exchange chromatography, and obtained pure GTX-4 (0.40 +/- 0.002) mg, GTX-1 (5.95 +/- 0.03) x 10(-2) mg, GTX-3 (6.92 +/- 0.05) x 10(-4) mg and GTX-2 (0.11 +/- 0.005) mg.
  
  
  CONCLUSIONS
  Pure gonyautoxins can be obtained by means of gel filtration chromatography and ion exchange chromatography from ethanol extracts of cultured Alexandriun minutum Halim Amtk2 strain.
authors.affiliation: School of Pharmacy, Fu Dan University, Shanghai 200032, China.
authors.forename: Yu-ping
authors.initials: YP
authors.lastname: Miao
authors.forename: Hong-nong
authors.initials: HN
authors.lastname: Zhou
authors.forename: Ren
authors.initials: R
authors.lastname: Wen
mesh_terms: D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange; D004141:Dinoflagellida / Q000737:chemistry*; D008387:Marine Toxins / Q000737:chemistry / Q000302:isolation & purification; D015394:Molecular Structure; D012530:Saxitoxin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D008387:Marine Toxins; D012530:Saxitoxin; C013007:gonyautoxins