[Isolation and structure identification of chemical constituents from the seeds of Descurainia sophia (L.) Webb ex Prantl].
OBJECTIVE
To isolate and determine the structures of chemical constituents from the seeds of Descurainia sophia (L.) Webb ex Prantl.


METHODS
The chemical constituents were extracted from the seeds of Descurainia sophia (L.) Webb ex Prantl with 75% ethanol and purified by polyamide, silica gel, RP-C18 and Sephadex LH-20 on column chromatography. Chemical methods and spectroscopic methods, such as 1H and 13CNMR, HSQC, HMBC and TOCSY spectra were used for the structural identification.


RESULTS
Fifteen compounds were obtained. Twelve of them were identified as quercetin-3-O-beta-D-glucopyranosyl-7-O-beta-gentiobioside (I), kaempferol-3-O-beta-D-glucopyranosyl-7-O-beta-gentiobioside (II), isorhamnetin-3-O-beta-D-glucopyranosyl-7-O-beta-gentiobioside (III), quercetin-7-O-beta-gentiobioside (IV), kaempferol-7-O-beta-gentiobioside (V), isorhamnetin-7-O-beta-gentiobioside (VI), quercetin-3,7-di-O-beta-D-glucopyranoside (VII), kaempferol-3, 7-di-O-beta-D-glucopyranoside (VIII), isorhamnetin-3, 7-di-O-beta-D-glucopyranoside (IX), kaempferol-3-O-beta-D-glucopyranosyl-7-O-[(2-O-trans-sinnapoyl)-beta-D- glucopyranosyl(1-->6)]-beta-D-glucopyranoside) (X), sinapic acid ethyl ester (XI) and 3, 4, 5-trimethoxyl-cinnamic acid (XII).


CONCLUSIONS
Compounds X and VI are new compounds. IV, V, VII, VIII and IX were isolated from Cruciferae family for the first time. I, II, III were obtained from Descurania genus and XI, XII from D. sophia for the first time.
Wang
Ai-qin
AQ
Beijing University of Traditional Chinese Medicine, Beijing 100102, China.
Wang
Xiu-kun
XK
Li
Jun-lin
JL
Cui
Xiang-yu
XY
D019607:Brassicaceae / Q000737:chemistry*; D044948:Flavonols / Q000737:chemistry / Q000302:isolation & purification*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry
D044948:Flavonols; D005960:Glucosides; C488139:isorhamnetin-7-O-beta-gentiobioside; C488140:kaempferol-3-O-beta-D-glucopyranosyl-7-O-((2-O-trans-sinnapoyl)-beta-D- glucopyranosyl(1-6))-beta-D-glucopyranoside