Use of novel PCR primers specific to the genes of staphylococcal enterotoxin G, H, I for the survey of Staphylococcus aureus strains isolated from food-poisoning cases and food samples in Taiwan.
Data regarding the incidence of the newly found enterotoxigenic Staphylococcus aureus strains in food poisoning cases and in food samples were to date not available in Taiwan. In this study, PCR primers specific for the detection of SEG, H and I genes, i.e., seg, seh and sei, were used for the assay of 55 human isolates of S. aureus negative to the classical enterotoxins (SEA-->SEE) detection. These isolates were from the fecal specimens of the patients suffering from food poisoning outbreaks. Only eight strains were found to have the seg, seh and sei. The presence of other bacterial pathogens, such as Vibrio parahaemolyticus, Bacillus cereus and Salmonella spp. and perhaps, strains producing other new staphylococcal enterotoxins, in the fecal specimens of these patients, may account for these food poisoning cases. For 139 strains from food samples, such as frozen Chinese foods, Chinese sausages and lunch meals, sea strains accounted for the major portion and it seemed to be the most common SE type to coexist with seg, seh and sei. Only two strains had sec and none of them had seg, seh or sei. For strains without the classical SE genes, only 13 strains had seg, seh and/or sei. The above results imply that seg, seh and sei S. aureus strains play only a minor role in food-borne outbreaks in Taiwan.
Chen
Tong-Rong
TR
Department of Food Science, National Chia-Yi University, Chia-Yi, Taiwan, ROC.
Chiou
Chien-Shun
CS
Tsen
Hau-Yang
HY
D017931:DNA Primers; D004269:DNA, Bacterial / Q000145:classification / Q000302:isolation & purification*; D004768:Enterotoxins / Q000235:genetics*; D005243:Feces / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D013202:Staphylococcal Food Poisoning / Q000175:diagnosis; D013211:Staphylococcus aureus / Q000302:isolation & purification* / Q000378:metabolism; D018089:Superantigens; D013624:Taiwan
D017931:DNA Primers; D004269:DNA, Bacterial; D004768:Enterotoxins; C402489:Staphylococcal enterotoxin H; D018089:Superantigens; C419397:enterotoxin G, staphylococcal; C419398:enterotoxin I, staphylococcal