Mechanism of 2-aminopurine-stimulated mutagenesis in Escherichia coli.
2-Aminopurine (2AP), a base analog, causes both transition and frameshift mutations in Escherichia coli. The analog is thought to cause mutations by two mechanisms: directly, by mispairing with cytosine, and indirectly, by saturation of mismatch repair (MMR). The goal of this work was to measure the relative contribution of these two mechanisms to the occurrence of transition mutations. Our data suggest that, in contrast to 2-aminopurine-stimulated frameshift mutations, the majority of transition mutations are a direct effect of base mispairing.
Pitsikas
Photini
P
Department of Biology, Concordia University, Montréal, Quebec, Canada.
Patapas
Jason M
JM
Cupples
Claire G
CG
D015075:2-Aminopurine; D001665:Binding Sites; D003596:Cytosine / Q000737:chemistry; D004260:DNA Repair; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000235:genetics*; D008954:Models, Biological; D016296:Mutagenesis; D009153:Mutagens; D009154:Mutation; D010957:Plasmids / Q000378:metabolism
D009153:Mutagens; D015075:2-Aminopurine; D003596:Cytosine