Polyethylenimine strategies for plasmid delivery to brain-derived cells.
The introduction of effective transfection reagents has had a dramatic impact on basic scientific studies over the past decade and is methodically becoming a clinical relevant agent. An area where these agents have had little impact to date is in transfection of neuronal cells either in vivo or in vitro. The poor results, obtained with these cells, likely arise from the innate properties of the cell itself such as its post-mitotic state and its fragility to the transfection agent. In this report, we investigated the transfection efficiency of branched and linear form of polyethylenimine (PEI) for a commonly used tissue culture cell line, the human CF bronchial epithelial cell line IB3-1, rat brain-derived glial, and neuronal cell lines. In addition, the effect of reaction conditions, such as ratio of PEI/plasmid, polymer molecular weight, and shape, was addressed on the transfection effects. The results indicate that branched PEI is more effective for the brain-derived cells. It is also shown that PEI 25 is more effective for the glial cells and PEI 50-100 is more effective for the neuronal cells under the evaluation conditions.
Zhang
Chun
C
Department of Pharmaceutics, University of Florida, Gainesville, FL 32601, USA.
Yadava
Preeti
P
Hughes
Jeffrey
J
D000818:Animals; D001921:Brain / Q000166:cytology*; D018929:Cell Culture Techniques; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D006801:Humans; D009457:Neuroglia / Q000166:cytology; D009474:Neurons / Q000166:cytology; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism*; D011094:Polyethyleneimine / Q000737:chemistry*; D051381:Rats; D014162:Transfection / Q000379:methods*
C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D011094:Polyethyleneimine