Effect of three xenobiotic compounds on Glutathione S-Transferase in the clam Ruditapes decussatus.
The effects of 4,4'DDE, methoxychlor and imidazole were studied on glutathione S-transferase activities in the gills and hepatopancreas of the clam Ruditapes decussatus. The contamination doses were 0.14 microM for 4,4'DDE, 0.14 microM for methoxychlor and 25 microM for imidazole. GST activities were spectrophotometrically measured. SDS-PAGE and isoelectric focusing (IEF) were used to separate the different GST isoforms in control and treated animals, followed by Western blotting performed with anti-alpha, anti-mu and anti-pi GST anti-sera. In the hepatopancreas, GST-CDNB activities were always two to five-fold lower than in the gills where the activities were significantly increased after exposure to 4,4'DDE (ca. 1.6-fold) and to methoxychlor (ca. 1.3-fold) compared to the controls (ca. 3 micromolmin(-1)mg(-1)protein) whereas they remained unchanged after treatment with imidazole. When using glutathione S-transferase anti-alpha, anti-mu and anti-pi anti-sera, a single 26 kDa polypeptide was observed in the hepatopancreas and in the gills in all the tested conditions. Five GST subunits were observed after IEF showing greater immuno-reactivity with the anti-pi mammalian class antiserum than with the anti-alpha or anti-mu mammalian anti-sera. One isoform of pI 5.77 was particularly induced by 4,4'DDE and methoxychlor; it was recognized by the three anti-sera tested and seemed to be more efficient in the gills than in the hepatopancreas. This isoform may play a role in organochlorine detoxication.
Hoarau
Pascal
P
UMR INRA-UNSA 1112 Response des Organismes aux Stress Environnementaux (R.O.S.E.), Faculté des Sciences, Université de Nice-Sophia Antipolis, Parc Valrose, BP 71, 06108 Nice, Cedex 2, France.
Garello
Ginette
G
Gnassia-Barelli
Mauricette
M
Roméo
Michèle
M
Girard
Jean-Pierre
JP
D000818:Animals; D049872:Bivalvia / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D015153:Blotting, Western; D003633:Dichlorodiphenyl Dichloroethylene / Q000633:toxicity; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction / Q000187:drug effects; D005602:France; D005880:Gills / Q000378:metabolism*; D005982:Glutathione Transferase / Q000096:biosynthesis*; D043143:Hepatopancreas / Q000378:metabolism*; D007093:Imidazoles / Q000633:toxicity; D007106:Immune Sera; D007525:Isoelectric Focusing; D008731:Methoxychlor / Q000633:toxicity; D012623:Seawater; D013053:Spectrophotometry; D015262:Xenobiotics / Q000633:toxicity*
D007093:Imidazoles; D007106:Immune Sera; D015262:Xenobiotics; D003633:Dichlorodiphenyl Dichloroethylene; C029899:imidazole; D005982:Glutathione Transferase; D008731:Methoxychlor