A keratin K5Cre transgenic line appropriate for tissue-specific or generalized Cre-mediated recombination.
We describe here a mouse line bearing a bovine keratin K5Cre recombinase transgene. These mice showed a dual pattern of Cre-mediated recombination, depending on the parent transmitting the transgene. In paternal transmission, recombination occurred specifically in the skin and stratified epithelia-as expected according to the expression of endogenous keratin K5. However, constitutive recombination between loxP sites transmitted by the sperm took place when the mother possessed the K5Cre transgene, even when the transgene was absent in the progeny. Cre expression in late-stage oocytes, with the Cre protein persisting into the developing embryo, leads to the constitutive recombination observed. Thus, this transgenic line allows for both tissue-specific and generalized recombination, depending on the breeding scheme.
Ramirez
Angel
A
Epithelial Damage, Repair and Tissue Engineering Program, CIEMAT, Madrid, Spain.
Page
Angustias
A
Gandarillas
Alberto
A
Zanet
Jennifer
J
Pibre
Sophie
S
Vidal
Miguel
M
Tusell
Laura
L
Genesca
Anna
A
Whitaker
Duncan A
DA
Melton
David W
DW
Jorcano
Jose L
JL
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D017931:DNA Primers; D005455:Fluorescent Antibody Technique; D005838:Genotype; D007633:Keratins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D045522:Recombinases / Q000378:metabolism*; D011995:Recombination, Genetic
D017931:DNA Primers; D045522:Recombinases; D007633:Keratins