title: Phospholipids and oxophospholipids in atherosclerotic plaques at different stages of plaque development.
abstract: We identified and quantified the hydroperoxides, hydroxides, epoxides, isoprostanes, and core aldehydes of the major phospholipids as the main components of the oxophospholipids (a total of 5-25 pmol/micromol phosphatidylcholine) in a comparative study of human atheroma from selected stages of lesion development. The developmental stages examined included fatty streak, fibrous plaque, necrotic core, and calcified tissue. The lipid analyses were performed by normal-phase HPLC with on-line electrospray MS using conventional total lipid extracts. There was great variability in the proportions of the various oxidation products and a lack of a general trend. Specifically, the early oxidation products (hydroperoxides and epoxides) of the glycerophosphocholines were found at the advanced stages of the plaques in nearly the same relative abundance as the more advanced oxidation products (core aldehydes and acids). The anticipated linear accumulation of the more stable oxidation products with progressive development of the atherosclerotic plaque was not apparent. It is therefore suggested that lipid infiltration and/or local peroxidation is a continuous process characterized by the formation and destruction of both early and advanced products of lipid oxidation at all times. The process of lipid deposition appears to have been subject to both enzymatic and chemical modification of the normal tissue lipids. Clearly, the appearance of new and disproportionate old lipid species excludes randomness in any accumulation of oxidized LDL lipids in atheroma.
authors.affiliation: Banting and Best Department of Medical Research, University of Toronto, Toronto, Canada M5G 1L6.
authors.forename: Amir
authors.initials: A
authors.lastname: Ravandi
authors.forename: Saeid
authors.initials: S
authors.lastname: Babaei
authors.forename: Ramsey
authors.initials: R
authors.lastname: Leung
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Monge
authors.forename: George
authors.initials: G
authors.lastname: Hoppe
authors.forename: Henry
authors.initials: H
authors.lastname: Hoff
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kamido
authors.forename: Arnis
authors.initials: A
authors.lastname: Kuksis
mesh_terms: D001011:Aorta / Q000473:pathology; D001161:Arteriosclerosis / Q000378:metabolism* / Q000473:pathology*; D002851:Chromatography, High Pressure Liquid; D018450:Disease Progression; D006801:Humans; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000378:metabolism; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000032:analysis / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis* / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D008077:Lipoproteins, LDL; D010713:Phosphatidylcholines; D010743:Phospholipids; C099252:oxidized low density lipoprotein