Monitoring sub-nanogram amount of acetylspiramycin in human urine using flow injection analysis with chemiluminescence detection.
OBJECTIVE
To establish a new and simple flow injection method for the rapid determination of acetylspiramycin (ASPM).


METHODS
ASPM was determined by chemiluminescence (CL) method combined with flow injection (FI) technology, which was based on the inhibitive effect of ASPM on the chemiluminescence reaction of the luminol-K3Fe (CN)6 system.


RESULTS
The decrease of chemiluminescence intensity was proportional to the logarithm of ASPM concentration (0.1-100) microgram.L-1, the detection limit was 40 ng.L-1 (3 sigma). The whole process, including sampling and washing, could be completed in 0.5 min with a RSD less than 3.0% (n = 5).


CONCLUSIONS
The FI-CL method is of both high sensitivity and good selectivity giving a throughput of 120 h-1. The proposed method was applied successfully to the determination of ASPM in pharmaceutical preparations and human urine without any pre-treatment. It was found that the ASPM concentration reached its maximum after being orally administrated for two hours.
Luan
Xin-jun
XJ
Department of Chemistry, Northwest University, Xi'an 710069, China.
Song
Zheng-hua
ZH
Xiao
Zhen
Z
D000900:Anti-Bacterial Agents / Q000032:analysis / Q000097:blood / Q000652:urine*; D017022:Flow Injection Analysis; D006801:Humans; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry; D008297:Male; D008832:Microchemistry; D015572:Spiramycin / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine*
D000900:Anti-Bacterial Agents; C033163:acetylspiramycin; D008165:Luminol; D015572:Spiramycin