Effects of the molecular structure of two amphiphilic antidepressant drugs on the formation of complexes with human serum albumin.
Interactions of two amphiphilic antidepressant drugs, imipramine and desipramine hydrochlorides, with the blood protein human serum albumin (HSA) were investigated to gain an understanding of the effects of drug molecular structure on the complex formation of drug-protein molecules. To elucidate the mechanisms of such effects, the protein-antidepressant interactions in aqueous buffered solutions of pH 3.0 and 5.5 (isoelectric point of HSA = 4.9) were investigated using conductivity, zeta potential, and dynamic light scattering. An increase of the critical micelle concentration of both antidepressants was detected as a consequence of extensive binding to the protein. From zeta-potential measurements, the Gibbs energies of adsorption of the drugs onto the protein were derived using the proposed models of Kayes and Ottewill and Watanabe. Measurements of the hydrodynamic radii of HSA-antidepressant complexes as a function of the drug concentration have shown a gradual increase of size of a saturation rather than a denaturation process of the protein. A larger drug adsorption at pH 5.5 than at pH 3.0 was also observed, as a consequence of a more important specific binding at the former pH.
Taboada
Pablo
P
Departamento de Física de la Materia Condensada, Facultad de Física, Universidad de Santiago de Compostela, E-15782 Santiago de Compostela, Spain. fmpablo@usc.es
Gutiérrez-Pichel
Manuel
M
Mosquera
Víctor
V
D000327:Adsorption; D000929:Antidepressive Agents, Tricyclic / Q000737:chemistry*; D003891:Desipramine / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007099:Imipramine / Q000737:chemistry*; D015394:Molecular Structure; D012709:Serum Albumin / Q000737:chemistry*; D055672:Static Electricity
D000929:Antidepressive Agents, Tricyclic; D012709:Serum Albumin; D007099:Imipramine; D003891:Desipramine