title: [Determination of calycosin-7-O-beta-D-glucopyranoside in radix astragali by HPLC].
abstract: OBJECTIVE
  TO establish a RP-HPLC method for the determination of calycosin-7-O-beta-D-glucopyranoside in Radix Astragali, and to analyse the calycosin-7-O-beta-D-glucopyranoside content of ten samples of Radix Astragali, collected from different regions.
  
  
  METHODS
  A Polaris C18(250 mm x 4.6 mm, 5 microns) column was used and a mixture of methanol-water (30:70) was used as the mobile phase at a flow rate of 1.0 mL.min-1. The column temperature was 25 degrees C and the UV detection wavelength was 254 nm.
  
  
  RESULTS
  The calibration curve was in good linearity over the range of 0.0106-2.12 micrograms with the regression equation Y = 3035. 97 X - 14.85(r = 0.9999). The average recovery was 95.8% (n = 5, RSD = 1.3%).
  
  
  CONCLUSIONS
  The method is simple, quick, sensitive and reproducible. In all of the samples, the calycosin-7-O-beta-D-glucopyranoside contents differ markedly.
authors.affiliation: Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, China.
authors.forename: Chun-xia
authors.initials: CX
authors.lastname: Luo
authors.forename: Ping-chuan
authors.initials: PC
authors.lastname: Lin
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Gu
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.forename: Da-zheng
authors.initials: DZ
authors.lastname: Wu
authors.forename: Zheng-tao
authors.initials: ZT
authors.lastname: Wang
authors.forename: Zhi-bi
authors.initials: ZB
authors.lastname: Hu
mesh_terms: D027885:Astragalus propinquus / Q000737:chemistry* / Q000145:classification; D002681:China; D002851:Chromatography, High Pressure Liquid; D017753:Ecosystem; D005960:Glucosides / Q000032:analysis*; D007529:Isoflavones / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D013045:Species Specificity
chemical_list: D005960:Glucosides; D007529:Isoflavones; C488311:calycosin-7-O-glucopyranoside