Sensitive enzyme-linked immunosorbent assay with urine samples: a tool for surveillance of schistosomiasis japonica.
An enzyme-linked immunosorbent assay (ELISA) to detect antibodies to Schistosoma japonicum soluble egg antigens (SEA) in un-concentrated urine was developed. The urine ELISA was applied to samples collected in a schistosomiasis-endemic village in China. The levels of anti-SEA antibodies detected in urine correlated well with those obtained with paired serum samples (r = 0.694, p<0.0001). Among 129 serum ELISA positives, 112 (86.8%) were positive by urine ELISA, while all 40 serum ELISA negatives from a non-endemic area were negative. The levels of anti-SEA in urine samples were stable up to 8 weeks of storage at 37 degrees C, with sodium azide as a preservative. Therefore, ELISA with urine samples can be used for the surveillance of schistosomiasis.
Itoh
Makoto
M
Department of Parasitology Aichi Medical University School of Medicine, Nagakute, Aichi-ken, Japan.
Ohta
Nobuo
N
Kanazawa
Tamotsu
T
Nakajima
Yasuo
Y
Sho
Masanori
M
Minai
Masaru
M
Daren
Zhou
Z
Chen
Yan
Y
He
Hongbin
H
He
Yong-Kang
YK
Zhong
Zhinan
Z
D000909:Antibodies, Helminth / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000652:urine*; D011159:Population Surveillance / Q000379:methods; D012554:Schistosomiasis japonica / Q000175:diagnosis* / Q000517:prevention & control; D012680:Sensitivity and Specificity; D013048:Specimen Handling
D000909:Antibodies, Helminth; D007074:Immunoglobulin G