title: Effect of freeze-thawing and polyethylene glycol (PEG) lipid on fusion and fission of phospholipid vesicles.
abstract: The effect of freeze-thawing on the size of egg yolk phosphatidylcholine (EggPC) vesicles in the presence of 0-40 mol% distearoylphosphatidylethanolamine-polyethylene glycol 2000 (DSPE-PEG) was studied. Mean diameters of the vesicles fell into a range of 80-150 nm after 10 times freeze-thawing in spite of their original size. In the process of freeze-thawing, two opponent events, one is fission and the other is fusion, occurred at the same time. DSPE-PEG accelerated the fusion event.
authors.affiliation: Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
authors.forename: Supaporn
authors.initials: S
authors.lastname: Sriwongsitanont
authors.forename: Masaharu
authors.initials: M
authors.lastname: Ueno
mesh_terms: D005615:Freezing; D008081:Liposomes; D010743:Phospholipids / Q000032:analysis / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000032:analysis / Q000737:chemistry*
chemical_list: D008081:Liposomes; D010743:Phospholipids; D011092:Polyethylene Glycols