title: [Germination of basidiospores of Agaricus bisporus].
abstract: The type of dormancy and conditions necessary for germination of Agaricus bisporus basidiospores (BS) were studied. BS failed to germinate on starvation agar and required the presence of carbon and nitrogen (asparagine and/or glucose) sources in the medium. Upon 3-week storage, BS germinated after 4-5 days. Heat shock (20 min at 45 degrees C) and the decreased temperature facilitated activation of germination. Heterocyclic compounds stimulating germination of endogenously dormant spores, such as furfural, failed to activate germination. The data obtained suggested an endogenous dormancy of A. bisporus BS differing from zygospores of Mucorales. BS contained 17-19% lipids with a composition of fatty acids differing from those of pileus and stipe of the fruiting body. The soluble carbohydrates of the cytosol amounted to 12% dry spore weight and consisted of mannitol (74%) and trehalose (26%). Unlike BS stored at 2 degrees C, the BS stored for 5 months at 20 degrees C lost their ability to germinate, which correlated with a decrease in the content of trehalose.
authors.affiliation: Institute of Microbiology, Russian Academy of Sciences, Moscow, 117813 Russia. feofilov@inmi.soft
authors.forename: E P
authors.initials: EP
authors.lastname: Feofilova
authors.forename: V M
authors.initials: VM
authors.lastname: Tereshina
authors.forename: L V
authors.initials: LV
authors.lastname: Garibova
authors.forename: L A
authors.initials: LA
authors.lastname: Zav'ialova
authors.forename: A S
authors.initials: AS
authors.lastname: Memorskaia
authors.forename: N S
authors.initials: NS
authors.lastname: Maryshova
mesh_terms: D000364:Agaricus / Q000378:metabolism / Q000502:physiology*; D001216:Asparagine; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis; D003470:Culture Media; D005662:Furaldehyde; D005947:Glucose; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D013172:Spores, Fungal / Q000254:growth & development / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014199:Trehalose / Q000032:analysis
chemical_list: D002241:Carbohydrates; D003470:Culture Media; D008055:Lipids; D001216:Asparagine; D014199:Trehalose; D005662:Furaldehyde; D005947:Glucose