Analysis of PEG 400 and 4000 in urine for gut permeability assessment using solid phase extraction and gel permeation chromatography with refractometric detection.
We developed a treatment of urine samples allowing the analysis of two intestinal permeability markers: polyethylene glycol (PEG) 400 (highly diffusible; basal permeability indicator) and PEG 4000 (poorly diffusible; indicator of an abnormal increase of permeability) by a unique gel permeation chromatography (GPC) with refractometric detection. Urinary PEG were extracted using a mixed-bed resin composed of C2 and C18 layers. Permeability mean values determined in 11 human healthy subjects were 24.20 +/- 9.30% and 0.12 +/- 0.08% for, respectively, PEG 400 and 4000. The percentage of the PEG 4000 permeability value to the one of PEG 400 corresponded to an intestinal permeability index (IPI) of 0.52 +/- 0.35 expressing a low diffusion of this poorly permeability marker.
Loret
S
S
Department of Biochemistry and General Physiology, University of Liege, B-4000 Liege, Belgium. suzanne.loret@fundp.ac.be
Nollevaux
G
G
Remacle
R
R
Klimek
M
M
Barakat
I
I
Deloyer
P
P
Grandfils
C
C
Dandrifosse
G
G
D000328:Adult; D002850:Chromatography, Gel / Q000379:methods*; D005260:Female; D006801:Humans; D007408:Intestinal Absorption; D008297:Male; D010539:Permeability; D011092:Polyethylene Glycols / Q000032:analysis*; D012031:Refractometry / Q000379:methods*
D011092:Polyethylene Glycols