title: Comparison of cell viability on anorganic bone matrix with or without P-15 cell binding peptide.
abstract: Cell-extracellular cell matrix (ECM) interactions play a vital role in development including cytoskeletal structure, growth and differentiation. A 15 amino acid, non-RGD containing, peptide that is derived from type I collagen was tested for its ability to confer cell survival. Two routinely utilized bone graft materials, anorganic bone matrix (ABM) and ABM carrying the cell binding peptide P-15 were compared for cell viability and apoptosis. Anchorage-dependent human foreskin fibroblasts (HFF) or osteogenic MC3T3-E1 cells were seeded on ABM or ABM/P-15. After serum withdrawal, viability and level of apoptosis were significantly (p<0.05) improved for cells on ABM/P-15 compared to cells on ABM. Scanning electron microscopy confirmed large, spread cells on ABM/P-15 and smaller cells with apoptotic cellular blebs on ABM. In addition, viable cell attachment was significantly greater on cells cultured on ABM/P-15 compared with demineralized freeze-dried bone allograft. This is the first report that the P-15 cell binding peptide, when complexed with ABM, promotes cell survival. The P-15 peptide may modulate cell number and tissue structure by the enhancement of viable cell attachment and via the regulation of apoptosis.
authors.affiliation: Research and Development, Dentsply Friadent Ceramed, 12860 W. Cedar Drive, Lakewood, CO 80228, USA.
authors.forename: Tracey
authors.initials: T
authors.lastname: Hanks
authors.forename: Brent Lee
authors.initials: BL
authors.lastname: Atkinson
mesh_terms: D016475:3T3 Cells; D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry / Q000494:pharmacology; D018786:Bone Substitutes / Q000737:chemistry*; D016025:Bone Transplantation / Q000379:methods; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D003094:Collagen / Q000737:chemistry* / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007287:Inorganic Chemicals / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D010446:Peptide Fragments / Q000737:chemistry* / Q000494:pharmacology*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D018786:Bone Substitutes; D007287:Inorganic Chemicals; D010446:Peptide Fragments; C407062:cell-binding peptide P-15; D003094:Collagen