[Effect of nitric oxide donor (NaNO2) on the stability of non-transformed and malignant cells to ultraviolet and gamma-radiation].
A decreased sensitivity of the Chinese hamster cells (line V-79) to gamma-radiation under the influence of nitric oxide induction was shown elsewhere. This effect is connected hypothetically with post-radiation reparation of DNA. The investigation of the nitric oxide donor effect on sensitivity of these to UV-radiation is of interest, because this radiation is an important ecological factor of the environment. The question of retention of nitric oxide positive effect on UV and gamma-radiation sensitivity in malignant HeLa cells is no less actual, because these cells significantly differ from normal cells of line V-79. We demonstrated that the donor of nitric oxide enhances stability of the Chinese hamster cells (line V-79) to UV-radiation, as well as to gamma-radiation independently of the time of cell incubation with sodium oxide donor before or after irradiation. The inefficiency of nitric oxide as a factor increasing UV-stability of cells was shown for malignant HeLa cells. A 1 h long incubation of these cells with NO-donor before gamma-irradiation decreased the number of chromosome aberrations, and conversely, the addition of this agent to the HeLa cell culture after gamma-irradiation did not change the radiostability. It may be inferred that distinctions in behaviour of nitric oxide in cultures of V-79 and HeLa cells using UV-radiation may be explained by transformation of the latter special features of their damage, and by the following reparation.
Bol'shakova
O I
OI
St. Petersburg Nuclear Physics Institute RAS, Gatchina, Leningrad District.
Sverdlov
A G
AG
Timoshenko
S I
SI
Nikanorova
N G
NG
Bondarev
G N
GN
Grachev
S A
SA
D000818:Animals; D002460:Cell Line / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006367:HeLa Cells / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D006801:Humans; D012977:Sodium Nitrite / Q000494:pharmacology*; D013997:Time Factors; D014466:Ultraviolet Rays
D012977:Sodium Nitrite