Development of an inducible system to control and easily monitor gene expression in Lactococcus lactis.
This report describes the implementation and use of a maltose-inducible system for regulated gene expression in Lactococcus lactis. The system was established using Green Fluorescent Protein as reporter. The transcription of a gene of interest from the inducible promoter of pLS1RGFP plasmid vector can be easily monitored by fluorescence spectroscopy and microscopy. As an example, the lactococcal ribonuclease III was overproduced in an active form.
Viegas
S C
SC
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apartado 127, 2781-901 iras, Portugal.
Fernández De Palencia
P
P
Amblar
M
M
Arraiano
C M
CM
López
P
P
D015153:Blotting, Western; D017931:DNA Primers; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins; D013294:Lactococcus lactis / Q000235:genetics*; D008164:Luminescent Proteins; D008320:Maltose; D008856:Microscopy, Fluorescence; D010957:Plasmids / Q000235:genetics*; D043244:Ribonuclease III / Q000235:genetics
D017931:DNA Primers; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D008320:Maltose; D043244:Ribonuclease III