title: Successful gene transfer using adeno-associated virus vectors into the kidney: comparison among adeno-associated virus serotype 1-5 vectors in vitro and in vivo.
abstract: OBJECTIVE
  Gene transfer into the kidney has great potential as a novel therapeutic approach. However, an efficient method of gene transfer into the kidney has not been established. We explored the transduction efficiency of renal cells in vitro and in vivo using adeno-associated virus (AAV) serotype 1-5 vectors encoding the beta-galactosidase gene.
  
  
  METHODS
  In the in vitro study, rat kidney epithelial cell line NRK52E cells were transfected with AAV serotype derived vectors. In the in vivo study, AAV serotype derived vectors were selectively injected into the kidney using a catheter-based gene delivery system in rats and mice mimicking the clinical procedure. The efficiency of gene expression was histologically evaluated on the basis of the beta-galactosidase expression.
  
  
  RESULTS
  AAV serotype 1, 2, and 5 vectors transduced in rat kidney epithelial cell line NRK52E cells in vitro, whereas AAV serotype 3 or 4 vectors showed no transduction. In addition, the kidney-specific injection of AAV serotype 2 vectors successfully transduced in tubular epithelial cells, but not in glomerular, blood vessel, or interstitial cells in vivo, whereas the rest of the serotypes showed no transduction.
  
  
  CONCLUSIONS
  Since kidney-specific gene delivery via the renal artery by catheterization is highly feasible in humans, these findings provide useful information for promising strategies in renal gene therapy.
authors.affiliation: Division of Organ Replacement Research, Center for Molecular Medicine, Jichi Medical School, Tochigi, Japan. masafumi@jichi.ac.jp
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Takeda
authors.forename: Masafumi
authors.initials: M
authors.lastname: Takahashi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Mizukami
authors.forename: Eiji
authors.initials: E
authors.lastname: Kobayashi
authors.forename: Koichi
authors.initials: K
authors.lastname: Takeuchi
authors.forename: Yoji
authors.initials: Y
authors.lastname: Hakamata
authors.forename: Takashi
authors.initials: T
authors.lastname: Kaneko
authors.forename: Hisashi
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Chiharu
authors.initials: C
authors.lastname: Ito
authors.forename: Keiya
authors.initials: K
authors.lastname: Ozawa
authors.forename: Kenichi
authors.initials: K
authors.lastname: Ishibashi
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Matsuzaki
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Takata
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Asano
authors.forename: Eiji
authors.initials: E
authors.lastname: Kusano
mesh_terms: D000818:Animals; D002404:Catheterization; D002460:Cell Line; D000229:Dependovirus / Q000145:classification / Q000235:genetics*; D005822:Genetic Vectors / Q000008:administration & dosage; D007668:Kidney / Q000033:anatomy & histology / Q000378:metabolism*; D008297:Male; D051379:Mice; D051381:Rats; D011917:Rats, Inbred Lew; D014161:Transduction, Genetic / Q000379:methods*