Competitive inhibition of adherence of enterotoxigenic Escherichia coli, enteropathogenic Escherichia coli and Clostridium difficile to intestinal epithelial cell line Lovo by purified adhesin of Bifidobacterium adolescentis 1027.
OBJECTIVE
To observe competitive inhibition of adherence of enterotoxigenic Escherichia coli (ETEC), enteropathogenic Escherichia coli (EPEC) and Clostridium difficile (C. difficile) to intestinal epithelial cell line Lovo by purified adhesin of Bifidobacterium adolescentis 1027 (B. ado 1027).


METHODS
The binding of bacteria to intestinal epithelial cell line Lovo was counted by adhesion assay. The inhibition of adherence of ETEC, EPEC and C. difficile to intestinal epithelial cell line Lovo by purified adhesin of B. ado 1027 was evaluated quantitatively by flow cytometry.


RESULTS
The purified adhesin at the concentration of 10 microg/mL, 20 microg/mL and 30 microg/mL except at 1 microg/mL and 5 microg/mL could inhibit significantly the adhesion of ETEC, EPEC and C. difficile to intestinal epithelial cell line Lovo. Moreover, we observed that a reduction in bacterial adhesion was occurred with increase in the concentration of adhesin, and MFI (Mean fluorescent intensity) was decreased with increase in the concentration of adhesin.


CONCLUSIONS
The purified adhesin of B. ado 1027 can inhibit the adhesion of ETEC, EPEC and C. difficile to intestinal epithelial cell line Lovo in a dose-dependent manner.
Zhong
Shi-Shun
SS
Chinese PLA Institute of Digestive Disease, Nanfang Hospital, First Military Medical University, Guangzhou 510515, Guangdong Province, China. zdoctor@sohu.com
Zhang
Zhen-Shu
ZS
Wang
Ji-De
JD
Lai
Zhuo-Sheng
ZS
Wang
Qun-Ying
QY
Pan
Ling-Jia
LJ
Ren
Yue-Xin
YX
D018829:Adhesins, Bacterial / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D001644:Bifidobacterium; D002460:Cell Line; D016360:Clostridioides difficile / Q000472:pathogenicity / Q000502:physiology*; D003967:Diarrhea / Q000517:prevention & control; D004761:Enterocolitis, Pseudomembranous / Q000517:prevention & control*; D004926:Escherichia coli / Q000472:pathogenicity / Q000502:physiology*; D004927:Escherichia coli Infections / Q000517:prevention & control*; D005434:Flow Cytometry; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000382:microbiology; D014774:Virulence
D018829:Adhesins, Bacterial