Catecholaminergic control of NK cell cytolytic activity regulatory factors in the spleen.
We examined the effects of norepinephrine (NE), isoproterenol (ISO) and metaproterenol (MP) on natural killer (NK) cell cytolytic activity and on the protein and mRNA levels of the regulatory factors perforin and granzyme B and interferon-gamma (IFN-gamma) in splenocytes. NE, the beta-adrenergic agonist ISO, and the beta 2-selective-agonist MP all inhibited the protein and mRNA levels of perforin, granzyme B and mRNA levels of IFN-gamma. NE inhibited NK cell cytolytic activity as well. Furthermore, MP inhibition of granzyme B mRNA was blocked by the beta-antagonist nadolol. These data suggest that NE and beta-adrenergic agonists may inhibit NK cell cytolytic activity by regulating the production of perforin, granzyme B, and IFN-gamma in splenocytes.
Dokur
Madhavi
M
Endocrinology Program, Biomedical Division at the Center of Alcohol Studies and Department of Animal Sciences, Rutgers, The State University of New Jersey, 84 Lipman Drive, New Brunswick, NJ 08901-8525, USA.
Boyadjieva
Nadka
N
Sarkar
Dipak K
DK
D000322:Adrenergic Agonists / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western; D002395:Catecholamines / Q000494:pharmacology*; D003602:Cytotoxicity, Immunologic / Q000502:physiology; D053804:Granzymes; D007371:Interferon-gamma / Q000187:drug effects / Q000276:immunology; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000187:drug effects* / Q000276:immunology; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000187:drug effects; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012697:Serine Endopeptidases / Q000187:drug effects / Q000276:immunology; D013154:Spleen / Q000276:immunology*
D000322:Adrenergic Agonists; D002395:Catecholamines; D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; D012333:RNA, Messenger; D054353:Perforin; D007371:Interferon-gamma; D053804:Granzymes; C509215:Gzmb protein, rat; D012697:Serine Endopeptidases