title: Automated flow cytometric analysis of blood cells in cerebrospinal fluid: analytic performance.
abstract: We compared the performance of an automated method for obtaining RBC and WBC counts and WBC differential counts in cerebrospinal fluid (CSF) samples with the reference manual method. Results from 325 samples from 10 worldwide clinical sites were used to demonstrate the accuracy, precision, and linearity of the method. Accuracy statistics for absolute cell counts showed a high correlation between methods, with correlation coefficients for all reportable absolute counts greater than 0.9. Linearity results demonstrated that the method provides accurate results throughout the reportable ranges, including clinical decision points for WBCs of 0 to 10/microL. Interassay precision and intra-assay precision for the ADVIA 120 (Bayer HealthCare, Tarrytown, NY) method were acceptable at all levels. The ADVIA 120 CSF Assay enumerates and differentiates cells via flow cytometry in a minimally diluted sample, improving the analysis of typically hypocellular CSF samples. Study results demonstrate that the automated ADVIA 120 CSF Assay is an acceptable alternative to the labor-intensive manual method.
authors.affiliation: St Olaf's University Hospital, Trondheim, Norway.
authors.forename: Marthe W
authors.initials: MW
authors.lastname: Aune
authors.forename: Joanne L
authors.initials: JL
authors.lastname: Becker
authors.forename: Carlo
authors.initials: C
authors.lastname: Brugnara
authors.forename: William
authors.initials: W
authors.lastname: Canfield
authors.forename: David M
authors.initials: DM
authors.lastname: Dorfman
authors.forename: W
authors.initials: W
authors.lastname: Fiehn
authors.forename: Gena
authors.initials: G
authors.lastname: Fischer
authors.forename: Patricia
authors.initials: P
authors.lastname: Fitzpatrick
authors.forename: Timothy H
authors.initials: TH
authors.lastname: Flaming
authors.forename: Hilde-Kristin
authors.initials: HK
authors.lastname: Henriksen
authors.forename: Jolanta E
authors.initials: JE
authors.lastname: Kunicka
authors.forename: Karl J
authors.initials: KJ
authors.lastname: Lackner
authors.forename: Elaine
authors.initials: E
authors.lastname: Minchello
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Mullenix
authors.forename: Michael
authors.initials: M
authors.lastname: Myers
authors.forename: Annette
authors.initials: A
authors.lastname: Petersen
authors.forename: Wanda
authors.initials: W
authors.lastname: Ternstrom
authors.forename: Sandra J
authors.initials: SJ
authors.lastname: Wilson
mesh_terms: D001322:Autoanalysis / Q000295:instrumentation; D002555:Cerebrospinal Fluid / Q000166:cytology*; D004906:Erythrocyte Count / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D006403:Hematologic Tests / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D007958:Leukocyte Count / Q000379:methods*; D012015:Reference Standards; D015203:Reproducibility of Results