Isolation and purification of honokiol and magnolol from cortex Magnoliae officinalis by high-speed counter-current chromatography.
High-speed counter-current chromatography was used to isolate and purify honokiol and magnolol from cortex Magnoliae Officinalis (Magnolia officinalis Rehd. et Wils.), a plant used in the traditional Chinese medicine. A crude sample, 150 mg, was successfully separated with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:0.4:1:0.4, v/v), and the fractions were analyzed by high-performance liquid chromatography. The separation produced 80 and 45 mg of honokiol and magnolol with purities of 99.2 and 98.2%, respectively, in 2.5 h.
Wang
Xiao
X
College of Life Sciences, Shandong Agricultural University, 18 Daizong Street, Taian, Shandong 271018, China.
Wang
Yuqiang
Y
Geng
Yanling
Y
Li
Fuwei
F
Zheng
Chengchao
C
D001713:Biphenyl Compounds / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D017705:Lignans / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D031566:Magnolia / Q000737:chemistry*; D000498:Allyl Compounds; D010636:Phenols
D001713:Biphenyl Compounds; D017705:Lignans; C005498:magnolol; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols