High-performance liquid chromatography and thin-layer chromatography assays for Devil's Club (Oplopanax horridus).
Devil's root, Oplopanax horridus, is a widely used folk medicine in Alaska and British Columbia. The inner bark of the root and stem has been used to treat colds, cough, fever, and diabetes. The present study involves the development of high-pressure liquid chromatography (HPLC) and thin-layer chromatography (TLC) methods to detect the presence of trans-nerolidol and sterols in the root bark. The HPLC and TLC analytical methods presented are suitable for the characterization and identification of Oplopanax horridus.
Gruber
John W
JW
Department of Biology, University of the Sciences in Philadelphia, Philadelphia, PA 19104, USA.
Kittipongpatana
Nisit
N
Bloxton
James D
JD
Der Marderosian
Ara
A
Schaefer
Frederick T
FT
Gibbs
Ron
R
D027481:Araliaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy