title: Determination of the antiangiogenesis agent 2-methoxyestradiol in human plasma by liquid chromatography with ultraviolet detection.
abstract: A high-performance liquid chromatography (HPLC) assay was developed for the quantitative determination of 2-methoxyestradiol (2ME2) in human plasma. Sample pretreatment involved a solid-phase extraction of 1 ml aliquots of plasma with C(18) micro-columns. Separation was achieved on a Novapak C(18) column (300 mm x 3.9 mm i.d.; 4 microm PS) at room temperature at an isocratic flow rate of 1 ml/min with 50% acetonitrile in water. Detection was performed at a UV wavelength of 205 nm. Calibration curves were linear in the concentration range of 1-50 ng/ml. The accuracy and precision values obtained from three different sets of quality controls analyzed in replicates of four on four separate occasions ranged from 90.7 to 105.2 and 3.17 to 8.27%, respectively.
authors.affiliation: Clinical Pharmacology Research Core, National Cancer Institute, 9000 Rockville Pike, Building 10, Room 5A01, Bethesda, MD 20892, USA.
authors.forename: Nehal J
authors.initials: NJ
authors.lastname: Lakhani
authors.forename: Alex
authors.initials: A
authors.lastname: Sparreboom
authors.forename: William L
authors.initials: WL
authors.lastname: Dahut
authors.forename: Jürgen
authors.initials: J
authors.lastname: Venitz
authors.forename: William D
authors.initials: WD
authors.lastname: Figg
mesh_terms: D000077584:2-Methoxyestradiol; D020533:Angiogenesis Inhibitors / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004958:Estradiol / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D020533:Angiogenesis Inhibitors; D004958:Estradiol; D000077584:2-Methoxyestradiol