title: [Activation effects of BCR/ABL antigen on CML T cells mediated by protein transduction domain].
abstract: OBJECTIVE
  To study the activation effect of BCR/ABL antigen on T cells from CML patients mediated by protein transduction domain (PTD).
  
  
  METHODS
  The fused plasmid containing PTD gene and b3a2 bcr/abl gene of CML was constructed by genetic engineering technique and was expressed in E.coli. The PBMCs from CML patients were stimulated in-vitro with purified PTD-BCR/ABL antigen and then expression of the activation antigen CD25 on CD8(+) and CD4(+) T cells after stimulation was detected by flow cytometry (FCM).
  
  
  RESULTS
  After stimulation with 100 mg/L of PTD-BCR/ABL antigen (final concentration) for 4 days in-vitro, CD8(+) T cells were activated in 5 of 10 CML patients and CD4(+) T cells were activated in 2 of 10 patients. Both CD8(+) and CD4(+) T cells were activated simultaneously in one of them. However, neither CD4(+) nor CD8(+) T cells was activated in BCR/ABL antigen stimulation group as control.
  
  
  CONCLUSIONS
  Using a PTD-mediated antigen transduction system, exogenous BCR/ABL antigen can be transferred into APCs and be processed and presented onto surface of APCs to activate Ag-specific CD8(+) and CD4(+) T cells in-vitro. The strategy outlined in this paper may provide a new approach for priming Ag-specific CD8(+) and CD4(+) T cells in-vitro and immunotherapy of CML.
authors.affiliation: Department of Hematology of Tangdu Hospital,Fourth Military Medical University, Xi'an 710038,China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.forename: Ying-Min
authors.initials: YM
authors.lastname: Liang
authors.forename: Jin
authors.initials: J
authors.lastname: Zheng
authors.forename: Xue-Yi
authors.initials: XY
authors.lastname: Li
authors.forename: Ren-An
authors.initials: RA
authors.lastname: Chen
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Jiang
authors.forename: Rong-Li
authors.initials: RL
authors.lastname: Wu
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016827:CD8 Antigens; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism; D016044:Fusion Proteins, bcr-abl / Q000235:genetics; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive; D017434:Protein Structure, Tertiary
chemical_list: D016827:CD8 Antigens; D016044:Fusion Proteins, bcr-abl