title: [The difference between the positive rate of anti-HCV in the patients with severe viral hepatitis detected by the first generation or second generation enzyme linked-immunosorbent assay].
abstract: OBJECTIVE
  To compare the positive rate of antibody to hepatitis C virus (anti-HCV) in sera of patients with severe viral hepatitis between 1984-1990 year and 1997-2003 year.
  
  
  METHODS
  Serum anti-HCV was detected by enzyme linked-immunosorbent assay (ELISA). It was detected by the first generation (1st) ELISA (Ortho Co. USA) in 79 cases of severe viral hepatitis during 1984-1990 year, and it was detected by the second generation (2nd) ELISA (Xiamen Xingchuang Co. China) in 251 cases of severe viral hepatitis during 1997-2003 year.
  
  
  RESULTS
  The positive rate of serum anti-HCV was 51.9% detected by the 1st ELISA in 79 cases of severe viral hepatitis during 1984-1990 year, and it was 1.2% detected by the 2nd ELISA in 251 cases of severe hepatitis during 1997-2003 year (chi2 = 133.68, P </= 0.001).
  
  
  CONCLUSIONS
  To compare with the positive rate of serum antibody to hepatitis C virus in severe viral hepatitis detected by the 1st ELISA, it was lower that detected by the 2nd ELISA
authors.affiliation: The Viral Institute of Chongqing Medical University, Chongqing 400010, China.
authors.forename: Da-zhi
authors.initials: DZ
authors.lastname: Zhang
authors.forename: You-rong
authors.initials: YR
authors.lastname: Zhao
authors.forename: Quan-hai
authors.initials: QH
authors.lastname: Zhang
authors.forename: Zhi-yi
authors.initials: ZY
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Qin
authors.forename: Hua
authors.initials: H
authors.lastname: He
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhou
authors.forename: Shu-hua
authors.initials: SH
authors.lastname: Guo
authors.forename: Ding-feng
authors.initials: DF
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D018937:Hepatitis C Antibodies / Q000097:blood*; D006525:Hepatitis, Viral, Human / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis
chemical_list: D018937:Hepatitis C Antibodies