Preparation of alkylation agents for bulged DNA microenvironments.
A designed molecule with capacity to alkylate DNA bulges has been prepared from readily available starting materials. The spirocyclic template utilized was designed on the basis of established architectures, and equipped with a mustard alkylating group. Preliminary studies confirm alkylation of specific bulged sequences, paving the way for second generation substrates with higher affinity.
Fouad
Farid S
FS
Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA.
Xi
Zhen
Z
Goldberg
Irving H
IH
Jones
Graham B
GB
D000477:Alkylating Agents / Q000138:chemical synthesis* / Q000378:metabolism; D001483:Base Sequence / Q000502:physiology; D001665:Binding Sites / Q000502:physiology; D004247:DNA / Q000235:genetics* / Q000378:metabolism*; D009690:Nucleic Acid Conformation
D000477:Alkylating Agents; D004247:DNA