Expression of fibrinolytic and coagulation factors in cocultured human endothelial and smooth muscle cells.
Interactions between endothelial cells and smooth muscle cells are interesting from a tissue-engineering point of view. We have developed a coculture system that allows direct contact between these two cell types. The fibrinolytic factors PAI-1, tPA, and uPA and the coagulation factor TF, were studied at the gene level by RT-PCR and at the protein level by ELISA. Significant changes of all studied factors were seen at the gene level in cocultured endothelial cells. tPA and TF were upregulated 4- and 7-fold, respectively, and PAI-1 and uPA were downregulated 4- and 1.5-fold, respectively, compared with single-cultured controls. In cocultured smooth muscle cells alterations of PAI-1 and TF were significant, with a 1.5-fold upregulation of PAI-1 and a 2.5-fold downregulation of TF. Results at the protein level mirrored the gene expression results. These findings indicate that cocultured endothelial cells are rendered both hypercoagulative and hyperfibrinolytic.
Helenius
Gisela
G
Department of Surgery, Wallenberg Laboratory for Cardiovascular Research, Sahlgrenska University Hospital, Göteborg University, Se-413 45 Göteborg, Sweden.
Heydarkhan-Hagvall
Sepideh
S
Siegbahn
Agneta
A
Risberg
Bo
B
D001779:Blood Coagulation Factors / Q000235:genetics / Q000378:metabolism*; D002450:Cell Communication; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000378:metabolism*; D005343:Fibrinolytic Agents / Q000378:metabolism*; D006801:Humans; D009130:Muscle, Smooth / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D023822:Tissue Engineering
D001779:Blood Coagulation Factors; D005343:Fibrinolytic Agents; D012333:RNA, Messenger