title: Molecular analysis of T-cell repertoire in patients with graft-versus-host disease after allogeneic stem cell transplantation.
abstract: Complementarity-determining region (CDR3) size spectratyping has often been used to analyze the clonal expansion of T-cells. CDR3 size spectratyping has been useful in the analysis of the oligoclonal expansion of T-cells in virus infection, graft-versus-leukemia effect (GVL), graft-versus-host disease (GVHD), and immune reconstitution of T-cells after allogeneic stem cell transplantation (allo-SCT). We analyzed 26 T cell receptor (TCR)-beta-chain subfamilies (VB) in 25 patients who underwent allo-SCT. Fifteen of these patients developed acute GVHD (aGVHD). Many TCR-VB were skewed in the early stage. In these TCR-VB subfamilies, VB6 was most often skewed at the time of skin aGVHD. We then analyzed the average score of the complexity of 26 TCR-VB spectratypings in patients with or without cGVHD. The patients who developed chronic GVHD (cGVHD) had a lower average score of TCR-VB complexity than that of patients without cGVHD (P = 0.010). In particular. the patients who developed the quiescent type and de novo type of cGVHD from 4 months after allo- SCT had a lower average score of TCR-VB complexity at 3 months than that of the patients who had no cGVHD (P = 0.0055). These results suggest that we might be able to consider a possible development of cGVHD by analyzing TCR-VB spectratyping after allo-SCT.
authors.affiliation: Department of Hematology and Oncology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tsutsumi
authors.forename: Junji
authors.initials: J
authors.lastname: Tanaka
authors.forename: Yoko
authors.initials: Y
authors.lastname: Miura
authors.forename: Tomomi
authors.initials: T
authors.lastname: Toubai
authors.forename: Naoko
authors.initials: N
authors.lastname: Kato
authors.forename: Fumie
authors.initials: F
authors.lastname: Fujisaw
authors.forename: Nobuyasu
authors.initials: N
authors.lastname: Toyoshima
authors.forename: Shuiti
authors.initials: S
authors.lastname: Ota
authors.forename: A
authors.initials: A
authors.lastname: Mori
authors.forename: Masakatu
authors.initials: M
authors.lastname: Yonezumi
authors.forename: Koiji
authors.initials: K
authors.lastname: Chiba
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kondo
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hasino
authors.forename: Ryouji
authors.initials: R
authors.lastname: Kobayasi
authors.forename: Nobuo
authors.initials: N
authors.lastname: Masauji
authors.forename: Masaharu
authors.initials: M
authors.lastname: Kasai
authors.forename: Masahiro
authors.initials: M
authors.lastname: Asaka
authors.forename: Masahiro
authors.initials: M
authors.lastname: Imamura
mesh_terms: D000293:Adolescent; D000328:Adult; D001773:Blood Cells; D002648:Child; D022801:Complementarity Determining Regions / Q000032:analysis; D005260:Female; D006086:Graft vs Host Disease / Q000175:diagnosis / Q000276:immunology*; D006402:Hematologic Diseases / Q000150:complications / Q000628:therapy; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology*; D014184:Transplantation, Homologous / Q000276:immunology
chemical_list: D022801:Complementarity Determining Regions; D016693:Receptors, Antigen, T-Cell, alpha-beta