title: [Influence of epidermal growth factor and gonadotrophin on the in vitro maturation of human oocytes].
abstract: OBJECTIVE
  To study the effect of epidermal growth factor(EGF) and different concentrations of gonadotrophin (Gn) on the in vitro maturation of human oocytes.
  
  
  METHODS
  EGF was added to the vitro culture medium in order to observe the effect of Gn combined with or without EGF on the result of in vitro maturation. The concentrations of hCG and FSH were changed respectively to observe the difference between the results.
  
  
  RESULTS
  Adding EGF to the culture medium improved the maturation rate of oocyte significantly (P < 0.05). There was no difference between the results with different concentrations of hCG and FSH in the culture medium.
  
  
  CONCLUSIONS
  EGF can improve the results of the in vitro maturation of human oocytes by increasing the maturation rate significantly. Increasing the concentration of Gn does not influence the results of in vitro maturation.
authors.affiliation: Reproductive Medical Center, Shandong Provincial Hospital, Shandong University, Jinan, Shandong 250021, China. yuanyuantv@sina.com
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Zijiang
authors.initials: Z
authors.lastname: Chen
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhao
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.forename: Shuiying
authors.initials: S
authors.lastname: Ma
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Jingmei
authors.initials: J
authors.lastname: Hu
mesh_terms: D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D005306:Fertilization / Q000187:drug effects; D006062:Gonadotropins / Q000494:pharmacology*; D006801:Humans; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology
chemical_list: D006062:Gonadotropins; D004815:Epidermal Growth Factor