title: [Induction of specific humoral immune response in mice by recombinant mutant HBV s gene vaccine].
abstract: OBJECTIVE
  To construct the recombinant eukaryotic expression vector pCMV-S2. S + 145R(PR) containing mutant HBV s gene and detect the specific humoral immune response to the PR in mice.
  
  
  METHODS
  The PR was constructed by positional cloning of restriction endonuclease, and then it was transfected into human hepatocellular carcinoma cell line Hep G2 through electrotransformation. The antigenicity of PR was examined by EIA, ELISA and immunocytochemical staining. The PR and empty vector pcDNA3.0 were then used respectively to immunize intramuscularly 5 C57BL/6 mice, dosage being 100 pg purified plasmid each mouse. The titers of serum anti-HBs and anti-HBs2 antibodies were detected by ELISA.
  
  
  RESULTS
  In vitro experiment showed that mutant HBsAg could bind to anti-HBs antibody. The PR could induce anti-HBs and anti-HBs2 antibody production in immunized mice. But the appearance time of serum anti-HBs2 antibody one or two weeks earlier than that of serum anti-HBs antibody.
  
  
  CONCLUSIONS
  The expression product of PR had a good antigenicity, which can induce specific hu-moral immune response in C57BL/6 mice.
authors.affiliation: Department of Pathology, Changzheng Hospital, Shanghai 200003, China. gerllini@yahoo.com.cn
authors.forename: Jun-hui
authors.initials: JH
authors.lastname: Ge
authors.forename: Hui-min
authors.initials: HM
authors.lastname: Liu
authors.forename: Jin
authors.initials: J
authors.lastname: He
authors.forename: Yu-li
authors.initials: YL
authors.lastname: Li
authors.forename: Hong-yu
authors.initials: HY
authors.lastname: Yu
authors.forename: Zhong
authors.initials: Z
authors.lastname: Ye
authors.forename: Le-zhi
authors.initials: LZ
authors.lastname: Zhang
authors.forename: Yi-ping
authors.initials: YP
authors.lastname: Hu
mesh_terms: D000818:Animals; D005260:Female; D006510:Hepatitis B Antibodies / Q000097:blood*; D006514:Hepatitis B Surface Antigens / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D017325:Hepatitis B Vaccines / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D011498:Protein Precursors / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D011498:Protein Precursors; D014614:Vaccines, Synthetic; C050807:presurface protein 2, hepatitis B surface antigen