Activation of phospholipase D by osmotic cell swelling.
In response to osmotic cell swelling, Intestine 407 cells react with a rapid and transient activation of phospholipase D (PLD). To investigate the role of PLD during the regulatory volume decrease, cells were treated with 1-butanol resulting in a depletion of PLD substrates. Activation of volume-regulated anion channels, but not the cell swelling-induced release of taurine, was largely inhibited in the presence of low concentrations of 1-butanol. In addition, hypotonicity-induced exocytosis, ATP release and subsequent endocytosis were found to be largely abrogated. The results support a model of cell volume regulation in which PLD plays an essential role in the cell swelling-induced vesicle cycling and in the activation of volume-sensitive anion channels.
Tomassen
Sebastian F B
SF
Department of Biochemistry, Erasmus University Medical Center, P.O. Box 1738, 3000DR Rotterdam, The Netherlands.
van der Wijk
Thea
T
de Jonge
Hugo R
HR
Tilly
Ben C
BC
D020001:1-Butanol / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism; D002460:Cell Line; D048429:Cell Size / Q000187:drug effects / Q000502:physiology*; D003911:Dextrans / Q000737:chemistry / Q000378:metabolism; D004789:Enzyme Activation; D006801:Humans; D007038:Hypotonic Solutions / Q000494:pharmacology; D007422:Intestines / Q000166:cytology; D008856:Microscopy, Fluorescence; D009997:Osmotic Pressure; D010739:Phospholipase D / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D011868:Radioisotopes; D013654:Taurine / Q000378:metabolism
D003911:Dextrans; D007038:Hypotonic Solutions; D010743:Phospholipids; D011868:Radioisotopes; D013654:Taurine; D000255:Adenosine Triphosphate; D020001:1-Butanol; D010739:Phospholipase D