Global gene expression of cells attached to a tissue engineering scaffold.
A goal of tissue engineering is to produce a scaffold material that will guide cells to differentiate and regenerate functional replacement tissue at the site of injury. Little is known about how cells respond on a molecular level to tissue engineering scaffold materials. In this work we used oligonucleotide microarrays to interrogate gene expression profiles associated with cell-biomaterial interactions. We seeded collagen-glycosaminoglycan meshes, a widely used tissue engineering scaffold material, with human IMR-90 fibroblasts and compared transcript levels with control cells grown on tissue culture polystyrene. Genes involved in cell signaling, extracellular matrix remodeling, inflammation, angiogenesis and hypoxia were all activated in cells on the collagen-GAG mesh. Understanding the impact of a scaffold on attached cells will facilitate the design of improved tissue engineering materials.
Klapperich
Catherine M
CM
Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. catherin@bu.edu
Bertozzi
Carolyn R
CR
D018958:CD55 Antigens / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D002460:Cell Line; D019743:Chemokines, CXC / Q000235:genetics; D011508:Chondroitin Sulfate Proteoglycans / Q000235:genetics; D003094:Collagen / Q000494:pharmacology*; D005293:Ferritins / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects* / Q000235:genetics; D006025:Glycosaminoglycans / Q000494:pharmacology*; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics; D015850:Interleukin-6; D016209:Interleukin-8 / Q000235:genetics; D053605:Leukemia Inhibitory Factor; D009363:Neoplasm Proteins / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010088:Oxidoreductases; D011137:Polystyrenes / Q000494:pharmacology; D011257:Pregnancy Proteins / Q000235:genetics; D011506:Proteins / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D013879:Thioredoxins / Q000235:genetics; D019700:Thrombospondin 1 / Q000235:genetics; D023822:Tissue Engineering; D019717:Tissue Inhibitor of Metalloproteinase-3; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics; D053675:Versicans
D018958:CD55 Antigens; D002352:Carrier Proteins; D019743:Chemokines, CXC; D011508:Chondroitin Sulfate Proteoglycans; D006025:Glycosaminoglycans; C405620:HILPDA protein, human; D018960:Hyaluronan Receptors; D015850:Interleukin-6; D016209:Interleukin-8; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D011137:Polystyrenes; D011257:Pregnancy Proteins; D011506:Proteins; C088390:TIMP3 protein, human; C486715:TXNIP protein, human; D019700:Thrombospondin 1; D019717:Tissue Inhibitor of Metalloproteinase-3; D019714:Tissue Inhibitor of Metalloproteinases; C508322:VCAN protein, human; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C058506:collagen-glycosaminoglycan copolymer; D053675:Versicans; D013879:Thioredoxins; D012313:RNA; D003094:Collagen; D005293:Ferritins; C454461:FTH1 protein, human; D010088:Oxidoreductases