title: [Study on immunoreaction induced by DCs loaded with hepatocarcinoma antigen peptide in-vitro].
abstract: OBJECTIVE
  To investigate the potency and antitumor effect of specific cytotoxic T lymphocytes induced by hepatocarcinoma antigen peptide EPVTKAEML-loaded dendritic cells (DCs).
  
  
  METHODS
  The healthy donors of HLA-B7 genotype were selected by PCR-SSP. Isolated and cultured DCs from HLA-B7 donors' spleen tissues were loaded with the peptide EPVTKAEML from hepatocarcinoma cell line HHCC. The specific CTL reaction and inhibitory effect of anti-HLA-I molecule mAb on CTL cytotoxicity were detected in-vitro by (51)Cr-release assay.
  
  
  RESULTS
  4 donors with HLA-B7 genotype were found. Their spleen T lymphocytes could be induced to proliferate and differentiate into specific CTLs by DCs loaded with the peptide. Antibody-blocking test demonstrated that the cytotoxity of CTLs was strictly limited by MHC-I molecule.
  
  
  CONCLUSIONS
  EPVTKAEML-loaded DCs can induce efficient and specific antitumor immune reaction in-vitro.
authors.affiliation: Department of Pathology, Fourth Military Medical University,Xi'an 710032 China.
authors.forename: Ping
authors.initials: P
authors.lastname: Qu
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Sui
authors.forename: Ai-Lin
authors.initials: AL
authors.lastname: Guo
authors.forename: Yun-Xin
authors.initials: YX
authors.lastname: Cao
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Dong
authors.forename: Shao-Ying
authors.initials: SY
authors.lastname: Lu
authors.forename: Jing
authors.initials: J
authors.lastname: Ye
authors.forename: Xiu-Min
authors.initials: XM
authors.lastname: Zhang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D045744:Cell Line, Tumor; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000276:immunology; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D010455:Peptides