One-step analysis of testis steroidogenesis from neonatal exposure to synthetic estrogen by normal-phase HPLC.
Neonatal exposure to synthetic estrogen endocrine disruptors or estrogen-receptor inhibitors induces developmental abnormalities in the male reproductive system. To investigate whether neonatal exposure affects spermatogenesis in juvenile and pubertal testis, Sprague-Dawley rat pups were given synthetic estrogen endocrine disruptors or estrogen-receptor inhibitors by a single injection on the day of birth at concentrations ranging between 2 to 40 mm, and sacrificed on day 21 (juvenile), 35 (prepuberty) or 50 (puberty). The testes were weighed and examined histologically at each stage. Further, the metabolites of steroidogenesis were analyzed using normal-phase high performance liquid chromatography. Neonatal exposure significantly reduced testis weights and steroidogenesis to one- fifth to one-half of that of the juvenile control, and further suppressed irreversible steroidogenesis and spermatogenesis during puberty.
Kuwada
Masahiro
M
Department of Biochemistry, School of Medicine Kitasato University, 1-15-1 Kitasato, Sagamihara, Kanagawa 228-0829, Japan. kuwada@med.kitasato-u.ac.jp
Kawashima
Rei
R
Furudate
Senich
S
Sugano
Sachiko
S
Maki
Jun
J
D000818:Animals; D000831:Animals, Newborn; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004054:Diethylstilbestrol / Q000494:pharmacology; D004702:Endocrine Glands / Q000187:drug effects; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D008297:Male; D009929:Organ Size / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000037:antagonists & inhibitors / Q000378:metabolism; D012715:Sesame Oil; D012741:Sexual Maturation; D013091:Spermatogenesis / Q000187:drug effects; D013256:Steroids / Q000032:analysis / Q000096:biosynthesis*; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
D004967:Estrogens; D011960:Receptors, Estrogen; D013256:Steroids; D004054:Diethylstilbestrol; D012715:Sesame Oil