Atrazine degradation in anaerobic environment by a mixed microbial consortium.
Atrazine degradation by anaerobic mixed culture microorganism in co-metabolic process and in absence of external carbon and nitrogen source was studied at influent atrazine concentration range of 0.5-15 mg/l. Wastewater of desired characteristic was prepared by the addition of various constituents in distilled water spiked with atrazine. In co-metabolic process, dextrose of various concentrations (150-2000 mg/l) was supplied as external carbon source. The reactors were operated in sequential batch mode in which 20% of treated effluent was replaced by the same amount of fresh wastewater everyday, thus maintaining a hydraulic retention time (HRT) equal to 5 days. In co-metabolic process, 40-50% of influent atrazine degradation was observed. First-order atrazine degradation rate (expressed in day(-1)) was better in co-metabolic process (5.5 x 10(-4)) than in absence of external carbon source (2.5 x 10(-5)) or carbon and nitrogen source (1.67 x 10(-5)). In presence of 2000 mg/l of dextrose, atrazine degradation was between 8% and 15% only. Maximum atrazine degradation was observed from wastewater containing 300 mg/l of dextrose and 5mg/l of atrazine. Influent atrazine concentration did not have much effect on the methanogenic bacteria which was clear from methane gas production and specific methanogenic activity (SMA).
Ghosh
Pranab Kumar
PK
Department of Civil Engineering, Vellore Institute of Technology, Vellore, Pin-632 014, India.
Philip
Ligy
L
D001280:Atrazine / Q000032:analysis / Q000378:metabolism*; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D004783:Environmental Microbiology; D007700:Kinetics; D008697:Methane / Q000378:metabolism; D017672:Nitrogen Compounds / Q000378:metabolism; D012722:Sewage / Q000382:microbiology; D014865:Waste Disposal, Fluid / Q000379:methods; D018508:Water Purification / Q000379:methods*
D003470:Culture Media; D017672:Nitrogen Compounds; D012722:Sewage; D002244:Carbon; D008697:Methane; D001280:Atrazine