title: Structure analysis of PH1161 protein, a transcriptional activator TenA homologue from the hyperthermophilic archaeon Pyrococcus horikoshii.
abstract: The crystal structure of the Bacillus subtilis TenA-homologue protein PH1161 from the hyperthermophilic archaebacterium Pyrococcus horikoshii was determined. TenA is known to belong to a new family of activators that stimulate the production of extracellular proteases in B. subtilis. A sequence-similarity search revealed that TenA-homologue proteins are widespread in bacteria and archaea, suggesting that this family of proteins plays an essential role in these organisms. In the present study, the first three-dimensional structure of a member of the TenA family of proteins was determined, unexpectedly revealing that the protein has a fold identical to that of haem oxygenase-1. Analysis has also shown that the protein has a unique ligand-binding pocket. Electron density of a bound ligand molecule was observed in this pocket. These results provide a valuable insight into the functional understanding of the TenA family of proteins.
authors.affiliation: Frontier Research Center for Post-genomic Science and Technology, Hokkaido University, Sapporo 001-0021, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Itou
authors.forename: Min
authors.initials: M
authors.lastname: Yao
authors.forename: Nobuhisa
authors.initials: N
authors.lastname: Watanabe
authors.forename: Isao
authors.initials: I
authors.lastname: Tanaka
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000378:metabolism; D001665:Binding Sites; D004583:Electrons; D006419:Heme Oxygenase (Decyclizing) / Q000737:chemistry; D051547:Heme Oxygenase-1; D008024:Ligands; D008958:Models, Molecular; D015233:Models, Statistical; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D043902:Pyrococcus horikoshii / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D015534:Trans-Activators / Q000737:chemistry*; D015533:Transcriptional Activation
chemical_list: D008024:Ligands; D015534:Trans-Activators; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1