Genetic and phenotype changes following in vitro interactions between Helicobacter pylori strains.
BACKGROUND
The purpose of the present paper was to determine whether in vitro interaction between different Helicobacter pylori strains leads to changes in antibiotic susceptibility, cagA, vacAM2 and DNA fingerprint patterns.


METHODS
Three H. pylori strains with known antibiotic susceptibility, cagA, vacAM2 status and polymerase chain reaction-random amplified polymorphic DNA (PCR-RAPD) fingerprint analysis were suspended in phosphate buffered saline (PBS pH 7.0), and the suspensions were mixed in equal proportion prior to culture on chocolate agar plates. Subcultures were performed five times every 3 days. As a control, each of the three strains was also subcultured separately. Antibiotic susceptibility testing, PCR for cagA, vacAM2 and PCR-RAPD analysis were done.


RESULTS
Surviving strain of the two H. pylori strains in each combination demonstrated change in resistance to both antibiotics but no change in sensitivity. CagA status of the surviving strain varied as compared to the vacAM2 status, which did not change. The PCR-RAPD fingerprint showed unique band pattern.


CONCLUSIONS
DNA transformation follows in vitro interaction. Helicobacter pylori strain with antibiotic resistance is likely to dominate in such in vitro interactions between various strains.
Yakoob
Javed
J
Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha, Hunan, China. yakoobjaved@hotmail.com
Fan
Xuegong
X
Hu
Guoling
G
Zhang
Zheng
Z
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis*; D016172:DNA Fingerprinting; D005838:Genotype; D016480:Helicobacter pylori / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D019105:Random Amplified Polymorphic DNA Technique
D000900:Anti-Bacterial Agents; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080866:cagA protein, Helicobacter pylori