Putrescine as a marker of the effects of 2-chloropropionic acid in the rat brain.
The neurotoxin 2-chloropropionic acid (2CPA, 750 mg/kg, per os) induces ataxia in rats causing neuropathological changes (necrosis and edema) localized mainly in the cerebellum (CB). It has been described that putrescine (PUT) is a good marker of severe brain damage. We measured the concentration of PUT (by HPLC) in ataxic rat brains 3 days after 2CPA dosing. PUT was 9-fold higher than normal values in CB, 5-fold higher in midbrain (MB) and medulla oblongata + pons (MO) and 3-fold higher in the remaining areas studied. Treatment with glycerol, a reducer of brain edema, lowered the concentration of PUT only in CB, MB and MO. Histological damage was found in CB and the spinal trigeminal nucleus (located in the pontomedullar brainstem). We suggest that PUT can act as a marker of both neuronal necrosis and brain edema.
de Vera
Núria
N
Department of Pharmacology and Toxicology, Institut d'Investigacions Biomèdiques de Barcelona (IIBB-CSIC, IDIBAPS), Barcelona, Spain. nvcfat@iibb.csic.es
Camón
Lluïsa
L
Martínez
Emili
E
D000704:Analysis of Variance; D000818:Animals; D015415:Biomarkers; D001921:Brain / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism; D001929:Brain Edema / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005990:Glycerol / Q000627:therapeutic use; D006652:Histological Techniques / Q000379:methods; D006843:Hydrocarbons, Chlorinated; D008297:Male; D011422:Propionates / Q000633:toxicity*; D011700:Putrescine / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D015415:Biomarkers; D006843:Hydrocarbons, Chlorinated; D011422:Propionates; C021974:2-chloropropionic acid; D005990:Glycerol; D011700:Putrescine