Evaluation of the preventive effect of isoflavone extract on bone loss in ovariectomized rats.
To examine a potential role for soybean phytoestrogens in postmenopausal bone loss, twenty-four 12-week-old Sprague-Dawley rats were divided randomly into 4 groups and given controlled diets for 16 weeks. The treatment groups were as followed: sham operated, ovariectomized (OVX) control, OVX + isoflavone extract (6.25 g/kg), and OVX + 17beta-estradiol (4 mg/kg). OVX treatments reduced femoral and fourth lumbar vertebral bone density and mineral content (p<0.01), decreased uterine weight (p<0.01), accelerated body weight increases (p<0.05), and increased the activities (p<0.01) of both serum alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP). Supplementation with isoflavone prevented the losses of bone density and mineral content caused by OVX (p<0.01). Although both isoflavone and 17beta-estradiol exhibited similar bone-sparing ability on the OVX-induced bone loss, the effect of isoflavone was not the same as that of 17beta-estradiol on the serum ALP and TRAP, body weight increase, and uterine weight change. We concluded that dietary supplementation with soybean isoflavone can prevent postmenopausal bone loss via a different mechanism of estrogen in OVX rats.
Lee
Yoon-Bok
YB
Central Research Institute, Dr Chung's Food Co, Ltd, Korea. leehyjo@snu.ac.kr
Lee
Hyong Joo
HJ
Kim
Kang Sung
KS
Lee
Jae-Yong
JY
Nam
Sang-Yoon
SY
Cheon
Sang-Hee
SH
Sohn
Heon-Soo
HS
D000135:Acid Phosphatase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D015519:Bone Density; D002118:Calcium / Q000032:analysis; D004435:Eating; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D019833:Genistein / Q000032:analysis; D006801:Humans; D007527:Isoenzymes / Q000097:blood; D007529:Isoflavones / Q000032:analysis / Q000737:chemistry / Q000627:therapeutic use*; D009929:Organ Size; D010024:Osteoporosis / Q000188:drug therapy / Q000517:prevention & control*; D015663:Osteoporosis, Postmenopausal / Q000188:drug therapy / Q000517:prevention & control; D010052:Ovariectomy; D010936:Plant Extracts / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013025:Glycine max / Q000737:chemistry*; D000071681:Tartrate-Resistant Acid Phosphatase
D007527:Isoenzymes; D007529:Isoflavones; D010936:Plant Extracts; D004958:Estradiol; C004742:daidzein; C086566:glycitein; D019833:Genistein; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D002118:Calcium