A mutation in the 5,10-methylenetetrahydrofolate reductase gene is not associated with preeclampsia in women of southeast Mexico.
BACKGROUND
We investigated the possible association between C677T-5,10-methylenetetrahydrofolate (MTHFR) polymorphism and preeclampsia in women from the Yucatan Peninsula in Mexico.


METHODS
We performed a comparative study among three groups of subjects: 148 preeclamptic women; 177 women with normal pregnancies, and 313 healthy volunteers as control group. All subjects were genotyped for C677T polymorphism in MTHFR gene by PCR amplification and digestion of the product with Hinf I restriction enzyme. Genotypic and allelic frequencies were compared between affected women (preeclamptic) and nonaffected groups to determine the association using Fisher exact test. Odds ratios (ORs) were also calculated to obtain estimation of relative risk (RR) for developing preeclampsia.


RESULTS
Statistical analysis of intergroup comparisons among preeclamptic women, women with normal pregnancies, and control group showed no statistically significant differences for any genotype or for T allele (p >0.05).


CONCLUSIONS
Our results suggest that C677T-MTHFR polymorphism is not an associated risk factor for developing preeclampsia in southeast Mexico. Results also confirmed high prevalence of C677T mutation in Mexico.
Pérez-Mutul
José
J
Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán, Mérida, Yucatán, Mexico. pmutul@tunku.uady.mx
González-Herrera
Lízbeth
L
Sosa-Cabrera
Tayde
T
Martínez-Olivares
Ramón
R
D000293:Adolescent; D000328:Adult; D000483:Alleles; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D009154:Mutation; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D011225:Pre-Eclampsia / Q000235:genetics; D011247:Pregnancy; D012306:Risk; D012307:Risk Factors
D042965:Methylenetetrahydrofolate Reductase (NADPH2)