[Expressions of c-fos in different brain regions after microinjection of 5-HT3 receptors agonist in hippocampus].
OBJECTIVE
To explore the neuronal circuit and possible pathway related to the 5-HT3 receptors in hippocampus neuroimmunomodulation.


METHODS
SABC immunocytochemical staining was used to detect the c-fos expression.


RESULTS
A large number of FOS-positive neurons expression were found in the hippocampus and brain cortex at 1 hour after microinjection of 5-HT3 receptor agonist (I-PBG) in the hippocampus, but as time went on, the number of FOS positive-neurons gradually decrease. A small number of FOS positive-neurons were found only in the hypothalamus and gray matter around acqueduct of midbrain, respectively at 8 and 16 hs after injection of 1-PBG. The expression of c-fos induced by 1-PBG could be blocked by pre-microinjection of tropisetron.


CONCLUSIONS
Brain cortex-hippocampus hypothalamus-gray matter around acqueduct of midbrain forma neuronal network, which may be related to immunomodulation.
Liu
She-lan
SL
Jiangsu Province Center for Disease Prevention and Control, Nanjing 210009, China.
Zhang
Xiao-yu
XY
Xu
De-yi
DY
D000818:Animals; D001645:Biguanides / Q000494:pharmacology*; D001923:Brain Chemistry / Q000187:drug effects*; D005260:Female; D006624:Hippocampus / Q000187:drug effects*; D007150:Immunohistochemistry; D008297:Male; D008845:Microinjections; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017366:Serotonin Receptor Agonists / Q000494:pharmacology*
D001645:Biguanides; D016760:Proto-Oncogene Proteins c-fos; D017366:Serotonin Receptor Agonists; C008846:phenyl biguanide