title: Production of monoclonal antibodies against canine leukocytes.
abstract: A panel of anti-canine leukocyte monoclonal antibodies (MAbs) was produced by immunizing BALB/c mice with canine peripheral blood mononuclear cells (PBMC), either resting or stimulated with concanavalin A (ConA). Three out of 28 clones-IH1, AB6, and HG6-screened by ELISA and producing antibody with the highest specificity for canine cell immunostaining, were subjected to three subsequent subcloning steps by limiting dilution, and selected for further characterization. These MAbs belonged to IgG1 (HG6 and IH1) and IgG2a (AB6) isotypes. The distribution of cell populations expressing the antigen recognized by the antibodies was identified by indirect immunoflorescence on canine PBMC and on tissue sections of lymph node, spleen, liver and skin. The possible crossreactivity with human PBMC was also examined in immunocytochemistry. One of the antibodies specifically recognized macrophages. The MAbs presented here can be foreseen as possible valuable diagnostic and research tools to study immune functions in dogs.
authors.affiliation: Laboratory of Molecular and Cellular Immunology, Gonçalo Moniz Research Center, Oswaldo Cruz Foundation, Bahia, Brazil. phpa@ufba.br
authors.forename: Paulo Henrique Palis
authors.initials: PH
authors.lastname: Aguiar
authors.forename: Roberto Robson
authors.initials: RR
authors.lastname: Borges dos Santos
authors.forename: Carla Andrade
authors.initials: CA
authors.lastname: Lima
authors.forename: Hilton
authors.initials: H
authors.lastname: Rios de Sousa Gomes
authors.forename: Daniela Farias
authors.initials: DF
authors.lastname: Larangeira
authors.forename: Patrícia Meira
authors.initials: PM
authors.lastname: Santos
authors.forename: Stella Maria
authors.initials: SM
authors.lastname: Barrouin-Melo
authors.forename: Washington Luis
authors.initials: WL
authors.lastname: Conrado dos-Santos
authors.forename: Lain
authors.initials: L
authors.lastname: Pontes-de-Carvalho
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003208:Concanavalin A / Q000276:immunology; D004285:Dogs; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007150:Immunohistochemistry; D007962:Leukocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D003208:Concanavalin A