Determination of fluquinconazole, pyrimethanil, and clofentezine residues in fruits by liquid chromatography with ultraviolet detection.
A simple method was developed for the determination of fluquinconazole, pyrimethanil, and clofentezine in whole fruit; peel; and pulp of mango, apple, and papaya. These compounds were extracted from fruit samples with a mixture of ethyl acetate-n-hexane (1 + 1, v/v). An aliquot (2 mL) of the extract was evaporated to near dryness under a stream of nitrogen, and the residue was dissolved with 2 mL methanol. The analysis was performed by means of liquid chromatography with ultraviolet detection at 254 nm using a gradient solvent system. The method was validated with fortified fruit samples at concentration levels of 0.05, 0.10, 0.20, and 0.50 mg/kg. Average recoveries (4-8 replicates) ranged from 80 to 95% with relative standard deviations between 3.5 and 12.7%. Detection limits ranged from 0.03 to 0.05 mg/kg for fruit pulp and 0.03 mg/kg for whole fruit. The quantitation limits ranged from 0.05 to 0.10 mg/kg for fruit pulp and 0.05 mg/kg for whole fruit. The analytical method was applied to fruit samples obtained from local markets.
Navickiene
Sandro
S
Universidade Estadual Paulista-Unesp, Departamento Química Orgânica, Instituto de Química, CP 355, 14800-900, Araraquara, São Paulo, Brazil.
Ribeiro
Maria Lúcia
ML
D002722:Chlorobenzenes / Q000032:analysis*; D002853:Chromatography, Liquid; D005638:Fruit / Q000737:chemistry*; D005659:Fungicides, Industrial / Q000032:analysis*; D011743:Pyrimidines / Q000032:analysis*; D015203:Reproducibility of Results
D002722:Chlorobenzenes; D005659:Fungicides, Industrial; D011743:Pyrimidines; C108337:pyrimethanil; C062106:clofentezine