title: Petrobacter succinatimandens gen. nov., sp. nov., a moderately thermophilic, nitrate-reducing bacterium isolated from an Australian oil well.
abstract: A novel Gram-negative, aerobic and moderately thermophilic bacterium, strain 4BON(T), was isolated from a non-water-flooded Australian terrestrial oil reservoir. Cells were non-spore-forming straight rods, which were motile by means of a polar flagellum. The optimum growth conditions were 55 degrees C, pH 6.9 and 0.5 % NaCl. Strain 4BON(T) was oxidase- and catalase-positive; it grew on fumarate, pyruvate, succinate, formate, ethanol and yeast extract in the presence of oxygen or nitrate as terminal electron acceptor. Nitrate was reduced to nitrous oxide. The DNA G+C content of the strain was 58.6 mol%. The closest phylogenetic relative of strain 4BON(T) was Hydrogenophilus thermoluteolus (similarity of 91.8 %), of the beta-Proteobacteria. As strain 4BON(T) is physiologically and phylogenetically different from H. thermoluteolus, it is proposed that it be assigned to a novel species of a novel genus, Petrobacter succinatimandens gen. nov., sp. nov. The type strain is 4BON(T) (=DSM 15512(T)=CIP 107790(T)).
authors.affiliation: IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, Marseille, France.
authors.forename: Monica Bonilla
authors.initials: MB
authors.lastname: Salinas
authors.affiliation: IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, Marseille, France.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fardeau
authors.affiliation: IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, Marseille, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Cayol
authors.affiliation: IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, Marseille, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Casalot
authors.affiliation: School of Biomolecular and Biomedical Sciences, Griffith University, Brisbane, Australia.
authors.forename: Bharat K C
authors.initials: BKC
authors.lastname: Patel
authors.affiliation: IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, Marseille, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Thomas
authors.affiliation: IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, Marseille, France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Garcia
authors.affiliation: IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, Marseille, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Ollivier
mesh_terms: D001315:Australia; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism; D010084:Oxidation-Reduction; D010578:Petroleum / Q000382:microbiology*; D010802:Phylogeny; D043362:Rhodocyclaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D009566:Nitrates; D010578:Petroleum