[Effect of lipid peroxide photooxidation on activity of lymphocyte-bound peroxidase].
The UV-light influence (240-390 nm) in the doses range of 75.5 divided by 2265 J/m2 has been studied on structurally functional state of lymphocytes by the method of determination of the TBA-active products, spectrophotometric method and enzyme-linked immunosorbent assay. It has been shown that UV-irradiation dose increase led to the intensification of lipids peroxide photooxidation processes on lymphocytes, accompanied by the increase of their natural peroxidase activity and ability to sorb exogenic peroxidase. The association of peroxidase with the native and UV-irradiated lymphocytes results in the decrease of its catalytic activity.
Artiukhov
V G
VG
Voronezh State University, Russia.
Gusinskaia
V V
VV
Ramil'tseva
E A
EA
D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000528:radiation effects*; D008214:Lymphocytes / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects*; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000378:metabolism*; D014466:Ultraviolet Rays
D006735:Horseradish Peroxidase; D009195:Peroxidase