Use of RAPD-PCR as a method to follow the progress of starter cultures in sauerkraut fermentation.
DNA fingerprinting methods were used to follow the progress of unmarked starter cultures in laboratory sauerkraut fermentations (1.2 and 13 l). Random prime PCR (RAPD-PCR) was used for strain-specific identification of Leuconostoc mesenteroides cultures. A comparative analysis of RAPD banding patterns for fermentation isolates and starter cultures was carried out using both genetically marked and unmarked cultures. While some variation in the RAPD patterns was observed, the results showed that the starter cultures dominated the fermentation during early heterofermentative stage for up to 5 days after the start of fermentation. Results from marked and unmarked starter cultures were confirmed by intergenic transcribed spacer (ITS)-PCR, and strain identify was confirmed by pulse field gel electrophoresis (PFGE) patterns. The results demonstrate the utility of RAPD to follow the progression of unmarked starter cultures of L. mesenteroides in sauerkraut fermentations.
Plengvidhya
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U.S. Department of Agriculture, Agricultural Research Service, NC State University, Raleigh, NC 27695-7624, USA.
Breidt
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Fleming
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HP
D001937:Brassica / Q000382:microbiology*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis; D021901:DNA, Intergenic / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D005285:Fermentation; D005516:Food Microbiology; D005784:Gene Amplification; D007934:Leuconostoc / Q000235:genetics / Q000302:isolation & purification*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*
D004269:DNA, Bacterial; D021901:DNA, Intergenic