title: Detection and quantitation of HBV DNA in the WHO International Standard for HIV-1 RNA (NIBSC code: 97/656).
abstract: Nucleic-acid amplification technology (NAT) assays have been implemented for HCV and HIV-1 in the United States, and many parts of Europe, Australia and Asia. Nucleic acid detection assays utilize many different technologies, and the WHO International Standards for nucleic acid tests are widely used to compare them. Currently, several laboratories are developing an assay for simultaneous detection of HCV RNA, HIV-1 RNA and HBV DNA. In the course of such development it was observed that the WHO International Standard for HIV-1 RNA (97/656) was positive for HBV DNA. In this report we confirm the presence of HBV DNA in the HIV-1 international standard through the qualitative Procleix-Ultrio assay. Further, using the TaqMan technology, through quantitative Bead Capture-TaqMan assay, we report that approximately 1000IU/ml dilution of HIV RNA contains approximately 4500IU/ml of HBV DNA.
authors.affiliation: Chiron Corporation, Blood Testing Division, 4560 Horton Street, 4.305, Emeryville, CA 94608, USA. v_shyamala@chiron.com
authors.forename: Venkatakrishna
authors.initials: V
authors.lastname: Shyamala
authors.forename: Joshua
authors.initials: J
authors.lastname: Cottrell
authors.forename: Phillip
authors.initials: P
authors.lastname: Arcangel
authors.forename: Dennis
authors.initials: D
authors.lastname: Madriaga
authors.forename: Jeff
authors.initials: J
authors.lastname: Linnen
authors.forename: Bruce
authors.initials: B
authors.lastname: Phelps
authors.forename: David
authors.initials: D
authors.lastname: Chien
mesh_terms: D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics / Q000592:standards; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods* / Q000592:standards; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics / Q000592:standards; D014773:Virology / Q000379:methods / Q000592:standards; D014944:World Health Organization
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral