[Inhibition of intracellular Ca(2+)-chelating agent on Hela cell apoptosis induced by HSV-1].
OBJECTIVE
To explore the variation of intracellular free-Ca (2+) concentration during Hela cell apoptosis induced by HSV-1 and inhibition of Ca(2+)-chelating agent on the apoptosis.


METHODS
Apoptosis of the Hela cells infected by HSV-1 was observed under scanning electron microscope (SEM) and transmission electron microscope (TEM), and the variation of intracellular free-Ca (2+) concentration, labeled with fluorescent probe was observed under fluorescence-microscope at a series of timepoints.


RESULTS
The Hela cells infected by HSV-1 showed typical apoptotic morphological changes. Free-Ca (2+) concentration increased and reached peak at the 12 hours after HSV-1 infection. Characteristic morphological features of apoptosis occurred at the 24 hours after infection. This apoptosis could be inhibited by intracellular Ca(2+)-chelating agent.


CONCLUSIONS
Intracellular free-Ca(2+) might play an important role in Hela cell apoptosis induced by HSV-1, which provides useful clues for clinical treatment of associated diseases.
Yang
Qiu-xia
QX
Department of Immunology, Harbin Medical University, Harbin 150086, China. yangqx_9051@yahoo.com
Jiang
Hong-chi
HC
Li
Bin
B
Xi
Xue-yan
XY
Liu
Yu
Y
Wang
Lan
L
Lu
Xue-ying
XY
Gu
Jin-yu
JY
Li
Dian-jun
DJ
D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000502:physiology*; D002614:Chelating Agents / Q000494:pharmacology*; D006367:HeLa Cells; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D006801:Humans; D008854:Microscopy, Electron
D002614:Chelating Agents; D002118:Calcium