Molecular analysis of T-cell repertoire in patients with graft-versus-host disease after allogeneic stem cell transplantation.
Complementarity-determining region (CDR3) size spectratyping has often been used to analyze the clonal expansion of T-cells. CDR3 size spectratyping has been useful in the analysis of the oligoclonal expansion of T-cells in virus infection, graft-versus-leukemia effect (GVL), graft-versus-host disease (GVHD), and immune reconstitution of T-cells after allogeneic stem cell transplantation (allo-SCT). We analyzed 26 T cell receptor (TCR)-beta-chain subfamilies (VB) in 25 patients who underwent allo-SCT. Fifteen of these patients developed acute GVHD (aGVHD). Many TCR-VB were skewed in the early stage. In these TCR-VB subfamilies, VB6 was most often skewed at the time of skin aGVHD. We then analyzed the average score of the complexity of 26 TCR-VB spectratypings in patients with or without cGVHD. The patients who developed chronic GVHD (cGVHD) had a lower average score of TCR-VB complexity than that of patients without cGVHD (P = 0.010). In particular. the patients who developed the quiescent type and de novo type of cGVHD from 4 months after allo- SCT had a lower average score of TCR-VB complexity at 3 months than that of the patients who had no cGVHD (P = 0.0055). These results suggest that we might be able to consider a possible development of cGVHD by analyzing TCR-VB spectratyping after allo-SCT.
Tsutsumi
Yutaka
Y
Department of Hematology and Oncology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
Tanaka
Junji
J
Miura
Yoko
Y
Toubai
Tomomi
T
Kato
Naoko
N
Fujisaw
Fumie
F
Toyoshima
Nobuyasu
N
Ota
Shuiti
S
Mori
A
A
Yonezumi
Masakatu
M
Chiba
Koiji
K
Kondo
Takeshi
T
Hasino
Satoshi
S
Kobayasi
Ryouji
R
Masauji
Nobuo
N
Kasai
Masaharu
M
Asaka
Masahiro
M
Imamura
Masahiro
M
D000293:Adolescent; D000328:Adult; D001773:Blood Cells; D002648:Child; D022801:Complementarity Determining Regions / Q000032:analysis; D005260:Female; D006086:Graft vs Host Disease / Q000175:diagnosis / Q000276:immunology*; D006402:Hematologic Diseases / Q000150:complications / Q000628:therapy; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology*; D014184:Transplantation, Homologous / Q000276:immunology
D022801:Complementarity Determining Regions; D016693:Receptors, Antigen, T-Cell, alpha-beta