[The influence of the extract of lumbricus on the production of NO and TNF-alpha by mouse MPhi and splenocytes].
OBJECTIVE
To explore the effect of the abstracts of lumbricus on the secretion of NO and TNF-alpha by mouse Mphi s and splenocytes.


METHODS
Murine Mphi s and spleen cell were co-cultured with various doses of lumbricus abstracts for 24 hours and then the supernate was collected. The levels of NO and TNF-alpha were detected by diazotization reaction and MTT colorimetry, respectively.


RESULTS
Compared with control group, 0.1 g/L of lumbricus abstracts could increase the NO level and antagonize the inhibition of dexamethasone(Dex). 1 x 10(-4), 1 x 10(-3) g/L of lumbricus abstracts could increase TNF-alpha level and also antagonize the inhibition of Dex on the secretion of TNF-alpha by Mphi s and splenic cells.


CONCLUSIONS
The abstracts of lumbricus can activate Mphi s and splenic cells to secrete NO and TNF-alpha and antagonize the inhibition effect of Dex on these cells.
Tang
Xiao-yun
XY
Department No. 1 of Experiment Technology Center, China Medical University, Shenyang 110001, China. tangxiaoyun00@yahoo.com.cn
Liang
Zai-fu
ZF
Jia
Xiu-kun
XK
Ji
Xiao-li
XL
Yang
Li-qun
LQ
D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000096:biosynthesis*; D009835:Oligochaeta; D010936:Plant Extracts / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D003907:Dexamethasone