title: Quantification of cow milk adulteration in goat milk using high-performance liquid chromatography with electrospray ionization mass spectrometry.
abstract: A method was developed for the quantification of cow milk adulteration in goat milk, based on solvent separation of whey proteins followed by high-performance liquid chromatography with electrospray ionization mass spectrometry (HPLC/ESI-MS). The presence of cow milk was determined using beta-lactoglobulin whey protein as the molecular marker. The adulterants were identified using both retention time and molecular mass derived from multiply charged molecular ions. Standard solutions containing cow and goat milk in different volume ratios were prepared and analyzed. Good linearity covering cow milk content from 5% and above was obtained. The proposed method identifies the adulterants using accurate molecular masses for protein identification and detects the addition of cow milk to goat milk at levels as low as 5%.
authors.affiliation: Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan 300.
authors.forename: Ren-Kun
authors.initials: RK
authors.lastname: Chen
authors.forename: Li-Wen
authors.initials: LW
authors.lastname: Chang
authors.forename: Ya-Yun
authors.initials: YY
authors.lastname: Chung
authors.forename: Ming-Hsung
authors.initials: MH
authors.lastname: Lee
authors.forename: Yong-Chien
authors.initials: YC
authors.lastname: Ling
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005504:Food Analysis / Q000379:methods* / Q000592:standards; D005506:Food Contamination / Q000032:analysis*; D006041:Goats; D007782:Lactoglobulins / Q000302:isolation & purification; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000302:isolation & purification; D012015:Reference Standards; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods* / Q000592:standards; D000067816:Whey Proteins
chemical_list: D007782:Lactoglobulins; D008894:Milk Proteins; D000067816:Whey Proteins