title: Quantitative detection of common deletion of mitochondrial DNA in hepatocellular carcinoma and hepatocellular nodular hyperplasia.
abstract: OBJECTIVE
  To study the deletion of mitochondiral DNA in hepatocellular carcinoma and hepatocellular nodular hyperplasia and its significance in the development of cancer.
  
  
  METHODS
  Deleted mtDNA (CD-mtDNA) and wild type mtDNA (WT-mtDNA) were quantitatively analyzed by using real-time PCR in 27 hepatocellular carcinomas (HCC) and corresponding noncancerous liver tissues and 27 hepatocellular nodular hyperplasiae (HNH).
  
  
  RESULTS
  A novel CD (4 981 bp) was detected in 85% (23/27) and 83%(22/27) of HCC and HNH tumor tissues, respectively, which were significantly higher than that in paired noncancerous liver tissues (57%, 15/27) (P<0.05). The CD/WT-mtDNA ratio in HCC tumors was 0.00092 (median, interquartile range, 0.0001202-0.00105), which was significantly higher than that in paired noncancerous liver tissues (median, 0.000, quartile range, 0-0) (P=0.002, Mann-Whitney Test), and was 25 of times of that in HNH tissues (median, 0.0000374, quartile range, 0-0.0004225) (P=0.002, Mann-Whitney test).
  
  
  CONCLUSIONS
  CD-mtDNA mutation plays an important role in the development and progression of HCC.
authors.affiliation: Department of Pathology, Cancer Center, Sun Yat-Sen University, Guangzhou 510060, Guangdong Province, China. jyshao@gzsums.edu.cn
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Shao
authors.forename: Hong-Yi
authors.initials: HY
authors.lastname: Gao
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: You-Yong
authors.initials: YY
authors.lastname: Lu
authors.forename: Yi-Xin
authors.initials: YX
authors.lastname: Zeng
mesh_terms: D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000453:epidemiology / Q000235:genetics*; D002681:China; D004272:DNA, Mitochondrial / Q000235:genetics*; D020518:Focal Nodular Hyperplasia / Q000453:epidemiology / Q000235:genetics*; D017353:Gene Deletion; D005819:Genetic Markers; D006801:Humans; D015994:Incidence; D008113:Liver Neoplasms / Q000453:epidemiology / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D004272:DNA, Mitochondrial; D005819:Genetic Markers