Enzymatic reactions by five chalcone synthase homologs from hop (Humulus lupulus L.).
The enzyme activities encoded in five cDNAs for chalcone synthase (CHS) homologs from hop were investigated. Only valerophenone synthase (VPS) and CHS_H1 showed both naringenin-chalcone and phlorisovalerophenone forming activity. Narigenin-chalcone production by VPS was much lower than by CHS_H1. Therefore, it is highly possible that flavonoid depends mainly on CHS_H1, while bitter acid biosynthesis depends mainly on VPS and CHS_H1.
Okada
Yukio
Y
Bioresources Research and Development Laboratories, Sapporo Breweries, Ltd, Gunma, Japan. yukio.okada@sapporobeer.co.jp
Sano
Yukie
Y
Kaneko
Takafumi
T
Abe
Ikuro
I
Noguchi
Hiroshi
H
Ito
Kazutoshi
K
D000214:Acyl Coenzyme A / Q000378:metabolism; D000217:Acyltransferases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D044950:Flavanones / Q000096:biosynthesis; D027582:Humulus / Q000201:enzymology* / Q000235:genetics
D000214:Acyl Coenzyme A; D044950:Flavanones; D000217:Acyltransferases; C020141:flavanone synthetase