Evolution of robusta green coffee redox enzymatic activities with maturation.
Oxidation reactions in coffee involve redox-sensitive polyphenols and appear to control the fragmentation of coffee storage proteins both in solution and during roasting. Coffee-specific nitrogenous flavor precursors may derive from this process. Accordingly, data converge to suggest that the redox status of the green bean before roasting might control the development of subsequent redox reactions during roasting. Consequently, we decided to identify biological events that may trigger or prevent oxidation during maturation of the coffee cherry and set the final redox status of the green bean. In a previous study, we observed that the sensitivity of green coffee to oxidative processes decreased along maturation. By using the very same samples originating from open-pollinated Robusta clones, we followed the activity of three essential redox enzymes: catalase (CAT), peroxidase (POD) and polyphenoloxidase (PPO). While CAT and POD activities increased with maturation, PPO activities decreased. Thanks to the identification of an atypical immature subclass, it appeared that CAT might be an essential factor in setting the final redox status of the green bean before the roasting event.
Montavon
Philippe
P
Nestec Ltd., Nestlé Research Centre, Vers-chez-les-Blanc, PO Box 44, CH-1000 Lausanne 26, Switzerland. philippe.montavon@rdls.nestle.com
Bortlik
Karlheinz
K
D002374:Catalase / Q000032:analysis; D004156:Catechol Oxidase / Q000032:analysis; D040503:Coffea / Q000201:enzymology* / Q000254:growth & development*; D005419:Flavonoids / Q000032:analysis; D005511:Food Handling / Q000379:methods; D006358:Hot Temperature; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000032:analysis; D010636:Phenols / Q000032:analysis; D059808:Polyphenols; D012639:Seeds / Q000737:chemistry* / Q000254:growth & development*
D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D004156:Catechol Oxidase; D002374:Catalase; D009195:Peroxidase