Differential regulation of FAK and PYK2 tyrosine phosphorylation after electroconvulsive shock in the rat brain.
It has been suggested that FAK and PYK2 have differential regulatory pathways and differential functions in the central nervous system. The authors have previously reported that electroconvulsive shock (ECS) activates PYK2 mediated signaling in the rat hippocampus. In the present article, the authors examined the effect of ECS on PYK2 and FAK mediated signaling in the rat cerebral cortex and hippocampus. Our results showed that ECS activated PYK2 more preferentially than FAK in both the cortex and the hippocampus. The association of Src-family kinases with FAK and PYK2 was also distinctively affected by ECS; Src was mainly associated with PYK2 while Yes was associated with FAK. The phosphorylation of FAK and PYK2 at the key tyrosine residue was not well correlated with the association with Src-family kinases.
Kang
Ung Gu
UG
Department of Psychiatry and Behavioral Science, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, Seoul 110-799, South Korea.
Jun
Sook Ja
SJ
Yoon
Se Chang
SC
Jeon
Songhee
S
Park
Joo-Bae
JB
Chung
Chun Kee
CK
Juhnn
Yong-Sung
YS
Kim
Yong Sik
YS
D000818:Animals; D002540:Cerebral Cortex / Q000201:enzymology*; D004597:Electroshock; D051417:Focal Adhesion Kinase 1; D051418:Focal Adhesion Kinase 2; D051416:Focal Adhesion Protein-Tyrosine Kinases; D006624:Hippocampus / Q000201:enzymology*; D008297:Male; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000502:physiology; D015854:Up-Regulation / Q000502:physiology; D019061:src-Family Kinases / Q000378:metabolism
D011505:Protein-Tyrosine Kinases; D051417:Focal Adhesion Kinase 1; D051418:Focal Adhesion Kinase 2; D051416:Focal Adhesion Protein-Tyrosine Kinases; C496052:Ptk2 protein, rat; C496054:Ptk2b protein, rat; D019061:src-Family Kinases