Insulin-induced changes of proteolytic activity of the lysosomal enzymes.
OBJECTIVE
Changes in the activity of alanine aminopeptidase, leucine aminopeptidase and cathepsins D and L in the liver and kidney of male and female of mice, injected with 0.4 IU/kg b.w. insulin for 4 and 8 days.


METHODS
The homogenates of the liver and kidney were taken for examination. The activity of alanine aminopeptidase, leucine aminopeptidase and cathepsins D and L has been determined according to [1] method.


RESULTS
The activity of alanine aminopeptidase, leucine aminopeptidase, cathepsins D and L in the liver and kidney of male and female of mice decreased in effect of insulin injections for 4 and 8 days.


CONCLUSIONS
The changes of enzyme activities showed a stimulating effect of the insulin injection on the labilization of lysosomal membranes. The range of the reaction remained in a relationship with the kind of the organ, the type of enzyme, time over which insulin introduced operates in the organism, and with the sex.
Witek
Bozena
B
Department of Genetics, Institute of Biology, Swietokrzyska Academy, Poland. b.witek@pu.kielce.pl
Ochwanowska
Ewa
E
Kolataj
Adam
A
Król
Teodora
T
Baranowska
Danuta
D
Rafay
Jan
J
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D018826:CD13 Antigens / Q000378:metabolism; D002402:Cathepsin D / Q000378:metabolism; D056668:Cathepsin L; D002403:Cathepsins / Q000378:metabolism; D003546:Cysteine Endopeptidases; D015536:Down-Regulation; D005260:Female; D007328:Insulin / Q000502:physiology*; D007668:Kidney / Q000201:enzymology; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D051379:Mice; D010447:Peptide Hydrolases / Q000378:metabolism*; D011897:Random Allocation
D001786:Blood Glucose; D007328:Insulin; D002403:Cathepsins; D010447:Peptide Hydrolases; D007931:Leucyl Aminopeptidase; D018826:CD13 Antigens; D003546:Cysteine Endopeptidases; D056668:Cathepsin L; C534278:Ctsl protein, mouse; D002402:Cathepsin D