title: Substitution of 3'-phosphate cap with a carbon-based blocker reduces the possibility of fluorescence resonance energy transfer probe failure in real-time PCR assays.
authors.affiliation: Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.
authors.forename: Kendall W
authors.initials: KW
authors.lastname: Cradic
authors.forename: Jason E
authors.initials: JE
authors.lastname: Wells
authors.forename: Lindsay
authors.initials: L
authors.lastname: Allen
authors.forename: Kent E
authors.initials: KE
authors.lastname: Kruckeberg
authors.forename: Ravinder J
authors.initials: RJ
authors.lastname: Singh
authors.forename: Stefan K G
authors.initials: SK
authors.lastname: Grebe
mesh_terms: D002244:Carbon; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D010755:Organophosphates / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic
chemical_list: D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D010755:Organophosphates; D011933:Reagent Kits, Diagnostic; D002244:Carbon