title: Using a customized DNA microarray for expression profiling of the estrogen-responsive genes to evaluate estrogen activity among natural estrogens and industrial chemicals.
abstract: We developed a DNA microarray to evaluate the estrogen activity of natural estrogens and industrial chemicals. Using MCF-7 cells, we conducted a comprehensive analysis of estrogen-responsive genes among approximately 20,000 human genes. On the basis of reproducible and reliable responses of the genes to estrogen, we selected 172 genes to be used for developing a customized DNA microarray. Using this DNA microarray, we examined estrogen activity among natural estrogens (17beta-estradiol, estriol, estrone, genistein), industrial chemicals (diethylstilbestrol, bisphenol A, nonylphenol, methoxychlor), and dioxin. We obtained results identical to those for other bioassays that are used for detecting estrogen activity. On the basis of statistical correlations analysis, these bioassays have shown more sensitivity for dioxin and methoxychlor.
authors.affiliation: Research Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
authors.forename: Shunichi
authors.initials: S
authors.lastname: Terasaka
authors.forename: Yukie
authors.initials: Y
authors.lastname: Aita
authors.forename: Akio
authors.initials: A
authors.lastname: Inoue
authors.forename: Shinichi
authors.initials: S
authors.lastname: Hayashi
authors.forename: Michiko
authors.initials: M
authors.lastname: Nishigaki
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Aoyagi
authors.forename: Hiroki
authors.initials: H
authors.lastname: Sasaki
authors.forename: Yuko
authors.initials: Y
authors.lastname: Wada-Kiyama
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Sakuma
authors.forename: Shuichi
authors.initials: S
authors.lastname: Akaba
authors.forename: Junko
authors.initials: J
authors.lastname: Tanaka
authors.forename: Hideko
authors.initials: H
authors.lastname: Sone
authors.forename: Junzo
authors.initials: J
authors.lastname: Yonemoto
authors.forename: Masao
authors.initials: M
authors.lastname: Tanji
authors.forename: Ryoiti
authors.initials: R
authors.lastname: Kiyama
mesh_terms: D001681:Biological Assay / Q000379:methods; D001943:Breast Neoplasms / Q000473:pathology; D004785:Environmental Pollutants / Q000494:pharmacology / Q000633:toxicity*; D004967:Estrogens / Q000494:pharmacology / Q000633:toxicity*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D011960:Receptors, Estrogen / Q000187:drug effects*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D004785:Environmental Pollutants; D004967:Estrogens; D011960:Receptors, Estrogen