Effect of rLcrV and rYopB from Yersinia pestis on murine peritoneal macrophages in vitro.
The interaction between macrophages and bacterial pathogens is crucial in the pathogenesis of infectious diseases. The 70 kb plasmid encodes low calcium response V (LcrV) or V antigen and a group of highly conserved yersinia outer proteins (Yops) are essential for full virulence. In present study, we investigated the effect of rLcrV and rYopB on macrophage functions in vitro. It is observed that rLcrV and rYopB inhibited the LPS induced expression of TNF-alpha, IFN-gamma, KC, IP-10, and IL-12 in macrophages. rLcrV and rYopB caused increased expression of IL-10 and TLR2, whereas inhibited TLR4 expression in LPS treated macrophages. IL-10 and TLR2 antibodies reversed the rLcrV and rYopB induced inhibition of TNF-alpha production by LPS treated macrophages, whereas IL-4 and TLR4 antibodies had no effect. Our data suggests a possible role of IL-10 and TLR2 in rLcrV and rYopB mediated inhibition of macrophage function.
Sharma
Rajesh Kumar
RK
School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
Sodhi
Ajit
A
Batra
Harsh Vardhan
HV
Tuteja
Urmil
U
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D000942:Antigens, Bacterial / Q000378:metabolism / Q000494:pharmacology*; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism / Q000494:pharmacology*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D054357:Chemokine CXCL10; D019743:Chemokines, CXC / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D019286:Endopeptidase K / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006358:Hot Temperature; D007371:Interferon-gamma / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D018664:Interleukin-12 / Q000235:genetics; D015847:Interleukin-4 / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D052899:Pore Forming Cytotoxic Proteins; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000235:genetics
D000906:Antibodies; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D054357:Chemokine CXCL10; D019743:Chemokines, CXC; C043908:LcrV protein, Yersinia; D008070:Lipopolysaccharides; D052899:Pore Forming Cytotoxic Proteins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; C493486:Tlr2 protein, mouse; C493487:Tlr4 protein, mouse; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; C078909:YopB protein, Yersinia; D016753:Interleukin-10; D018664:Interleukin-12; D015847:Interleukin-4; D007371:Interferon-gamma; D019286:Endopeptidase K