The glycosome membrane of Trypanosoma cruzi epimastigotes: protein and lipid composition.
Highly purified glycosomes from Trypanosoma cruzi epimastigotes were obtained by differential centrifugation and isopycnic ultracentrifugation. Glycosomal membranes, produced by carbonate treatment of purified glycosomes, exhibited about eight main protein bands and eight minor ones. Essentially the same protein pattern was observed in the detergent-rich fraction of a Triton X-114 fractionation of whole glycosomes, indicating that most of the membrane-bound polypeptides were highly hydrophobic. The orientation of these proteins was studied by in situ labelling followed by limited pronase hydrolysis of intact glycosomes. Three glycosome membrane proteins were characterized as peripheral by comparing the protein bands patterns of membrane fractions obtained by different treatments. Noteworthy membrane polypeptides were: (1) a peripheral 75k Da membrane protein, oriented towards the cytosol, which was the most abundant glycosomal membrane protein in exponentially growing epimastigotes but was essentially absent in stationary phase cells; (2) a pair of integral membrane proteins with molecular masses in the range of 85-100 kDa, which were only present in stationary phase cells; (3) a heme-containing 36k Da protein, strongly associated to the membrane, present in both growth phases; (4) a very immunogenic 41k Da integral membrane polypeptide, oriented towards the cytosol. The lipid composition of the glycosomal membranes was also investigated. The distribution of phospholipid species in glycosomes and glycosomal membranes was very similar to that of whole cells, with phosphatidyl-ethanolamine, phosphatidyl-choline, and phosphatidyl-serine as main components and smaller proportions of sphingomyelin and with phosphatidyl-inositol. On the other hand, glycosomes were enriched in endogenous sterols (ergosterol, 24-ethyl-5,7,22-cholesta-trien-3beta-ol), and precursors, when compared with whole cells, a finding consistent with the proposal that these organelles are involved in the de novo biosynthesis of sterols in trypanosomatids.
Quiñones
Wilfredo
W
Unidad de Bioquímica de Parásitos, Centro de Ingeniería Genética, Facultad de Ciencias, Universidad de Los Andes, Apartado 38, Mérida, Venezuela.
Urbina
Julio A
JA
Dubourdieu
Michel
M
Luis Concepción
Juan
J
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis; D002499:Centrifugation, Density Gradient; D007425:Intracellular Membranes / Q000737:chemistry / Q000648:ultrastructure; D008563:Membrane Lipids / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis*; D008830:Microbodies / Q000737:chemistry* / Q000648:ultrastructure; D015800:Protozoan Proteins / Q000032:analysis*; D014349:Trypanosoma cruzi / Q000737:chemistry* / Q000276:immunology / Q000648:ultrastructure; D014461:Ultracentrifugation
D000953:Antigens, Protozoan; D008563:Membrane Lipids; D008565:Membrane Proteins; D015800:Protozoan Proteins