title: Risk assessment of biocontrol products: a semi-selective medium for improving quantitative isolation of Paecilomyces lilacinus (strain 251) from soil.
abstract: Paecilomyces lilacinus (Thom) Samson 1974 is an egg pathogenic fungus, attacking mainly sedentary stages of root-knot and cyst nematodes, especially eggs, and is about to register as a product for the biological control of nematodes. Monitoring P. lilacinus (strain 251) is essential by way of ascertaining the fate and behaviour of this fungus in the soil. In order to obtain more data on the persistence of P. lilacinus (strain 251) in the soil, an improved selective medium for this strain is necessary. For this reason, different compounds with known antifungal properties were tested. Among them, P. lilacinus (strain 251) was found to tolerate high concentrations of 2,6-dichlor-4-nitroanilin, the active ingredient of Dichloran, which inhibits growth of many commonly occurring soil-borne fungi. The fact that P. lilacinus is more tolerant of higher salt concentrations than many other fungi are, was also taken into consideration. As a basis for the improved medium, OHIO-agar medium was used.
authors.affiliation: Institute for Plant Diseases, Phytopathology and Nematology in Soil Ecosystems, University of Bonn, Nussallee 9, D-53115 Bonn, Germany.
authors.forename: C I
authors.initials: CI
authors.lastname: Rumbos
authors.forename: R A
authors.initials: RA
authors.lastname: Sikora
authors.forename: S
authors.initials: S
authors.lastname: Kiewnick
mesh_terms: D000818:Animals; D001542:Benomyl / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D009348:Nematoda; D010143:Paecilomyces / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D010572:Pest Control, Biological / Q000379:methods* / Q000592:standards; D018570:Risk Assessment; D012988:Soil Microbiology
chemical_list: D003513:Cycloheximide; D001542:Benomyl