title: Products of the reaction between alpha- or gamma-tocopherol and nitrogen oxides analyzed by high-performance liquid chromatography with UV-visible and atmospheric pressure chemical ionization mass spectrometric detection.
abstract: The reaction products of alpha- or gamma-tocopherol with nitric oxide in the presence of molecular oxygen were isolated and characterized. The consumption of tocopherols and the formation of the major products were monitored by high-performance liquid chromatography (HPLC) by a gradient elution method. The quantitative analysis of these compounds with UV-Vis detectors, however, was interfered by several minor products having similar UV spectra and retention times as those of the major ones. In order to establish a quantitative analytical method for the products, we investigated other detection methods, and found that atmospheric pressure chemical ionization (APCI), LC-MS was a more selective and better analytical method for these compounds.
authors.affiliation: Department of Physical Chemistry, Kyoritsu University of Pharmacy, 1-5-30 Shibakoen, Minato-ku, Tokyo 105-8512, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Nagata
authors.forename: Yohta
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Hideko
authors.initials: H
authors.lastname: Kanazawa
mesh_terms: D001274:Atmospheric Pressure; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D009589:Nitrogen Oxides / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D024502:alpha-Tocopherol / Q000032:analysis* / Q000737:chemistry; D024504:gamma-Tocopherol / Q000032:analysis* / Q000737:chemistry
chemical_list: D009589:Nitrogen Oxides; D024504:gamma-Tocopherol; D024502:alpha-Tocopherol