Co-localization of CD3 and prion protein in Jurkat lymphocytes after hypothermal stimulation.
While long-term effects of temperature treatment in respect of, e.g., gene-expression and cellular function have already been studied in some detail, nothing is known on the physiological responses of lymphocytes during short-term hypothermal shifts. In this report, we characterized the effects of such a stimulation using the human lymphocyte cell line Jurkat E6.1 and present evidence that warming from 4 to 37 degrees C for only 2 min is sufficient to cause co-localization of CD3, prion protein and the lipid-raft ganglioside GM1 paralleling lymphocyte activation as observed by Ca(2+) mobilization and mitogen-activated protein kinase-phosphorylation.
Wurm
Susanne
S
Upper Austrian Research GmbH, Center for Biomedical Nanotechnology, Scharitzerstr. 6-8, 4020 Linz, Austria.
Paar
Christian
C
Sonnleitner
Alois
A
Sonnleitner
Max
M
Höglinger
Otmar
O
Romanin
Christoph
C
Wechselberger
Christian
C
D000199:Actins / Q000378:metabolism; D017252:CD3 Complex / Q000737:chemistry / Q000378:metabolism*; D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002784:Cholesterol / Q000172:deficiency / Q000378:metabolism; D003505:Cyclodextrins / Q000494:pharmacology; D015638:Cytochalasin D / Q000494:pharmacology; D004789:Enzyme Activation; D005455:Fluorescent Antibody Technique; D005677:G(M1) Ganglioside / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007036:Hypothermia, Induced; D019169:Jurkat Cells; D008213:Lymphocyte Activation; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D011328:Prions / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D013601:T-Lymphocytes / Q000378:metabolism*; D047392:beta-Cyclodextrins
D000199:Actins; D017252:CD3 Complex; D003505:Cyclodextrins; D011328:Prions; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D015638:Cytochalasin D; D005677:G(M1) Ganglioside; D002784:Cholesterol; D020928:Mitogen-Activated Protein Kinases; D002118:Calcium