title: Expression of transforming growth factor-beta isoforms in the skin, kidney, pancreas and bladder in a German shepherd dog affected by renal cystadenocarcinoma and nodular dermatofibrosis.
abstract: The present study was performed to assess the expression of isoforms 1, 2 and 3 of transforming growth factor (TGF)-beta in skin nodular dermatofibrosis lesions, kidney, bladder and pancreas from a 10-year-old female German shepherd dog (GSD) affected by renal cystadenocarcinoma and nodular dermatofibrosis (RCND) compared with normal GSDs (n = 2). Formalin-fixed, paraffin-embedded tissues obtained from the dog affected by RCND, diagnosed by renal ultrasonography and histopathological examination were analysed by immunohistochemistry using polyclonal antibodies to TGF-beta1, 2 and 3, and evaluated semiquantitatively using an immunoreactivity score. Similar expression of TGF-beta2 and TGF-beta3 was observed in all tissue specimens in both the RCND-affected animal and normal dogs. In contrast, TGF-beta1 immunoreactivity was increased in the derma of the RCND canine. Comparable TGF-beta1 serum levels were found between the diseased and normal animals. The increased local cutaneous production of TGF-beta1 in the RCND dog, compared with the normal animals, suggests that this cytokine may play an important role in the induction of nodular dermatofibrosis associated with renal cystadenocarcinoma.
authors.affiliation: Department of Animal Pathology, Faculty of Veterinary Medicine, University of Torino, Via Leonardo da Vinci 44, Grugliasco, Torino, Italy. ebaver@libero.it
authors.forename: A
authors.initials: A
authors.lastname: Vercelli
authors.forename: G
authors.initials: G
authors.lastname: Bellone
authors.forename: O
authors.initials: O
authors.lastname: Abate
authors.forename: G
authors.initials: G
authors.lastname: Emanuelli
authors.forename: A
authors.initials: A
authors.lastname: Cagnasso
mesh_terms: D000818:Animals; D016022:Case-Control Studies; D003536:Cystadenocarcinoma / Q000150:complications / Q000662:veterinary*; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D005260:Female; D018219:Histiocytoma, Benign Fibrous / Q000150:complications / Q000378:metabolism / Q000662:veterinary*; D007150:Immunohistochemistry / Q000662:veterinary; D007668:Kidney / Q000378:metabolism; D007680:Kidney Neoplasms / Q000150:complications / Q000662:veterinary*; D010179:Pancreas / Q000378:metabolism; D012867:Skin / Q000378:metabolism; D012878:Skin Neoplasms / Q000150:complications / Q000378:metabolism / Q000662:veterinary*; D016212:Transforming Growth Factor beta / Q000097:blood / Q000378:metabolism*; D001743:Urinary Bladder / Q000378:metabolism
chemical_list: D016212:Transforming Growth Factor beta