Sirius red is able to selectively stain eosinophil granulocytes in bovine, ovine and equine cervical tissue.
The aim of the present study was to examine the suitability of sirius red staining for selective light microscopic demonstration of eosinophil granulocytes in cervical tissue of mares, cows and sheep. For this purpose, tissue was fixed in 4% neutral buffered formol or in Bouin's solution. Paraffin sections of 5-microm thickness were stained with sirius red. In cows, mares and sheep a selective distinction of eosinophilic infiltration is successful after both fixation methods.
Wehrend
A
A
Klinik für Geburtshilfe, Gynäkologie und Andrologie der Gross- und Kleintiere, Giessen, Germany. Azel.Wehrend@vetmed.uni-giessen.de
Hetzel
U
U
Huchzermeyer
S
S
Klein
C
C
Bostedt
H
H
D000818:Animals; D001391:Azo Compounds; D002417:Cattle; D002584:Cervix Uteri / Q000166:cytology*; D004804:Eosinophils / Q000166:cytology*; D005260:Female; D006098:Granulocytes / Q000166:cytology*; D006736:Horses; D012756:Sheep; D013194:Staining and Labeling / Q000379:methods / Q000662:veterinary*
D001391:Azo Compounds; C062246:Direct Red 81