title: Murine plasmacytoid dendritic cells induce effector/memory CD8+ T-cell responses in vivo after viral stimulation.
abstract: Like their human counterparts, mouse plasmacytoid dendritic cells (pDCs) play a central role in innate immunity against viral infections, but their capacity to prime T cells in vivo remains unknown. We show here that virus-activated pDCs differentiate into antigen-presenting cells able to induce effector/memory CD8(+) T-cell responses in vivo against both epitopic peptides and endogenous antigen, whereas pDCs activated by synthetic oligodeoxynucleotides containing unmethylated cytosine-guanine motifs (CpG) acquire only the ability to recall antigen-experienced T-cell responses. We also show that immature pDCs are unable to induce effector or regulatory CD8(+) T-cell responses. Thus, murine pDCs take part in both innate and adaptive immune responses by directly priming naive CD8(+) T cells during viral infection.
authors.affiliation: Unité de Biologie des Régulations Immunitaires, Institut National de la Santé et de la Recherche Médicale, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris cedex 15, France.
authors.forename: Géraldine
authors.initials: G
authors.lastname: Schlecht
authors.forename: Sylvie
authors.initials: S
authors.lastname: Garcia
authors.forename: Nicolas
authors.initials: N
authors.lastname: Escriou
authors.forename: Antonio A
authors.initials: AA
authors.lastname: Freitas
authors.forename: Claude
authors.initials: C
authors.lastname: Leclerc
authors.forename: Gilles
authors.initials: G
authors.lastname: Dadaglio
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D000956:Antigens, Viral / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology*; D002454:Cell Differentiation; D002465:Cell Movement; D018899:CpG Islands / Q000502:physiology; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology* / Q000821:virology; D005260:Female; D006358:Hot Temperature; D007156:Immunologic Memory / Q000187:drug effects / Q000276:immunology*; D008297:Male; D051379:Mice; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D009975:Orthomyxoviridae / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology; D013154:Spleen / Q000276:immunology / Q000473:pathology
chemical_list: D000956:Antigens, Viral; D009838:Oligodeoxyribonucleotides; D010047:Ovalbumin