Quantitative detection of common deletion of mitochondrial DNA in hepatocellular carcinoma and hepatocellular nodular hyperplasia.
OBJECTIVE
To study the deletion of mitochondiral DNA in hepatocellular carcinoma and hepatocellular nodular hyperplasia and its significance in the development of cancer.


METHODS
Deleted mtDNA (CD-mtDNA) and wild type mtDNA (WT-mtDNA) were quantitatively analyzed by using real-time PCR in 27 hepatocellular carcinomas (HCC) and corresponding noncancerous liver tissues and 27 hepatocellular nodular hyperplasiae (HNH).


RESULTS
A novel CD (4 981 bp) was detected in 85% (23/27) and 83%(22/27) of HCC and HNH tumor tissues, respectively, which were significantly higher than that in paired noncancerous liver tissues (57%, 15/27) (P<0.05). The CD/WT-mtDNA ratio in HCC tumors was 0.00092 (median, interquartile range, 0.0001202-0.00105), which was significantly higher than that in paired noncancerous liver tissues (median, 0.000, quartile range, 0-0) (P=0.002, Mann-Whitney Test), and was 25 of times of that in HNH tissues (median, 0.0000374, quartile range, 0-0.0004225) (P=0.002, Mann-Whitney test).


CONCLUSIONS
CD-mtDNA mutation plays an important role in the development and progression of HCC.
Shao
Jian-Yong
JY
Department of Pathology, Cancer Center, Sun Yat-Sen University, Guangzhou 510060, Guangdong Province, China. jyshao@gzsums.edu.cn
Gao
Hong-Yi
HY
Li
Yu-Hong
YH
Zhang
Yu
Y
Lu
You-Yong
YY
Zeng
Yi-Xin
YX
D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000453:epidemiology / Q000235:genetics*; D002681:China; D004272:DNA, Mitochondrial / Q000235:genetics*; D020518:Focal Nodular Hyperplasia / Q000453:epidemiology / Q000235:genetics*; D017353:Gene Deletion; D005819:Genetic Markers; D006801:Humans; D015994:Incidence; D008113:Liver Neoplasms / Q000453:epidemiology / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D004272:DNA, Mitochondrial; D005819:Genetic Markers