title: Prevalence of Enterocytozoon bieneusi in post-weaned dairy calves in the eastern United States.
abstract: Fecal specimens were obtained from 3- to 8-month-old post-weaned dairy calves on farms in Vermont, New York, Pennsylvania, Maryland, Virginia, North Carolina, and Florida. After removal of fecal debris by sieving and density gradient centrifugation, 59 of 452 calves (13%) from 11 farms in six states were found positive for Enterocytozoon bieneusi by PCR and DNA sequence analysis. Based on gene sequence data this genotype of E. bieneusi found in post-weaned calves was 100% identical to that found in pre-weaned calves in North America and differed by only two positions in 1,069 base pairs from specimens analyzed from humans. However, compared with previous reports, the prevalence of E. bieneusi was significantly higher in post-weaned than in pre-weaned calves from many of the same farms.
authors.affiliation: Environmental Microbial Safety Laboratory, Animal and Natural Resources Institute Agricultural Research Service, United States Department of Agriculture, Building 173, MD 20705, Beltsville, USA.
authors.forename: Mónica
authors.initials: M
authors.lastname: Santín
authors.forename: James M
authors.initials: JM
authors.lastname: Trout
authors.forename: Ronald
authors.initials: R
authors.lastname: Fayer
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000469:parasitology; D016054:DNA, Protozoan / Q000032:analysis; D021862:Enterocytozoon / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D005243:Feces / Q000469:parasitology; D005838:Genotype; D016881:Microsporidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D014481:United States / Q000453:epidemiology; D014886:Weaning
chemical_list: D016054:DNA, Protozoan; D012335:RNA, Ribosomal