Cultivation of immobilized Dictyostelium discoideum for the production of soluble human Fas ligand.
Dictyostelium discoideum was immobilized by cultivation on inorganic porous matrices consisting of broken pumice or a ceramic catalyst carrier (CeramTec) to produce human soluble Fas ligand (hFasL). These supports were actively colonized by D. discoideum reaching cell (number) densities 10-20 times higher locally than those observed in suspension culture under similar conditions. In repeated batch or continuous operation, hFasL productivities of up to 15-25 microg h(-1) l(-1) pore volume were attained. The immobilized cell densities and hFasL productivities could be kept constant for a long period of time by repeated renewal or continuous feeding of complex or synthetic medium.
Lu
Yinghua
Y
Department of Chemical Engineering, Xiamen University, 361005, Fujian, PR China.
Knol
Jaco C
JC
Linskens
Maarten H K
MH
Friehs
Karl
K
van Haastert
Peter J M
PJ
Flaschel
Erwin
E
D000818:Animals; D001709:Biotechnology / Q000379:methods; D002452:Cell Count; D018914:Cells, Immobilized; D002516:Ceramics; D003470:Culture Media / Q000737:chemistry; D004023:Dictyostelium / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D053222:Fas Ligand Protein; D005947:Glucose / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis; D017640:Silicates
D003470:Culture Media; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D017640:Silicates; D005947:Glucose; C005144:pumice