title: Headspace solid-phase microextraction and capillary gas chromatographic-mass spectrometric determination of rivastigmine in canine plasma samples.
abstract: A simple, rapid and sensitive method for determination of rivastigmine in plasma samples was developed using headspace solid-phase microextraction (HS-SPME) and gas chromatography with mass spectrometry (GC-MS). The optimum conditions for the SPME procedure were: headspace extraction on a 65-microm polydimethylsiloxane/divinylbenzene (PDMS/DVB) fiber; 0.5 ml of plasma modified with 1.0 ml of sodium hydroxide-sodium carbonate solution (0.7 M:0.5M); extraction temperature of 100 degrees C, with stirring at 2000 rpm for 30 min. The calibration curve showed linearity in the range from 0.2 to 80 ng/ml with regression coefficient corresponding to 0.9965 and coefficient of the variation of the points of the calibration curve lower than 10%. The quantification limit for rivastigmine in plasma was 0.2 ng/ml. The method was applied to determination of rivastigmine in canine plasma samples from animals after a single oral administration.
authors.affiliation: Department of Pharmacy, Fudan University, Shanghai 200032, PR China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Sha
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.forename: Taomin
authors.initials: T
authors.lastname: Huang
authors.forename: Bei
authors.initials: B
authors.lastname: Yang
authors.forename: Gengli
authors.initials: G
authors.lastname: Duan
mesh_terms: D000818:Animals; D002800:Cholinesterase Inhibitors / Q000097:blood*; D004285:Dogs; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D018696:Neuroprotective Agents / Q000097:blood*; D048448:Phenylcarbamates / Q000097:blood*; D000068836:Rivastigmine
chemical_list: D002800:Cholinesterase Inhibitors; D018696:Neuroprotective Agents; D048448:Phenylcarbamates; D000068836:Rivastigmine