Does octreotide impair anastomotic healing after small bowel resection?
Seventy two rats underwent small bowel resection followed by end-to-end anastomosis. The octreotide group consisted of 36 rats treated with octreotide (subcutaneously, 7 microg/kg/day, in two equal doses), and the control group consisted of 36 rats treated with the same volume saline. Twelve animals from each group were re-explored on the 4th, 8th and 15th postoperative day. Leakages, adhesions, obstructions were the clinical postoperative findings identified and recorded. Anastomotic bursting pressures were measured. Histochemical studies included haematoxylin-eosin and Van Gieson staining techniques and focused on the microscopic characterization of the healing process. Adhesions, leakages and obstructions were not different between octreotide and control groups. Anastomoses of the octreotide group had increased bursting pressures on the 8th and the 15th day (p < .05 , p < .05, respectively). Regarding the histogical results, on the 8th day the octreotide group compared to the control group, showed healing in more layers (p < .05), increased bright red collagen fibers and quantity of fibroblasts (p < .05), and on the 15th day, the octreotide group showed fewer gaps (p < .05), increased bright red collagen fibers and quantity of fibroblasts (p < .05). In this experimental model, it appeared that octreotide does not impair healing of small bowel anastomoses, but in contrast, there is some evidence that it enhances healing on the 8th and the 15th postoperative day.
Papalambros
E
E
Department of Surgery, University of Athens Medical School, Laiko Hospital, Athens, Greece.
Filis
K
K
Angelopoulou
R
R
Diamantis
T
T
Tsigris
C
C
Bastounis
E
E
D000714:Anastomosis, Surgical; D000818:Animals; D001696:Biomechanical Phenomena; D013505:Digestive System Surgical Procedures / Q000379:methods*; D005765:Gastrointestinal Agents / Q000494:pharmacology*; D007421:Intestine, Small / Q000187:drug effects* / Q000601:surgery; D008297:Male; D023421:Models, Animal; D015282:Octreotide / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013529:Surgical Wound Dehiscence / Q000139:chemically induced; D014945:Wound Healing / Q000187:drug effects*
D005765:Gastrointestinal Agents; D015282:Octreotide