Interval immobilization technique for recognition toward a highly hydrophilic cyanobacterium toxin.
A novel adsorption medium containing selective molecular recognition site for one of the powerful cyanobacterium toxins, Cylindrospermopsin (CYN) was developed using a special technique, namely interval immobilization technique. The adsorption medium was prepared using molecular assembly derived from an alternative-template molecule coupled with functional monomers for fixing the interval between the ionic functional groups in CYN. As results of liquid chromatographic evaluations, selective molecular recognition ability for CYN was observed as expected. Further studies proved that the association constant for CYN on this medium was slightly higher than that on blank polymer.
Kubo
Takuya
T
Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
Hosoya
Ken
K
Watabe
Yoshiyuki
Y
Tanaka
Nobuo
N
Takagi
Hiroo
H
Sano
Tomoharu
T
Kaya
Kunimitsu
K
D000327:Adsorption; D001427:Bacterial Toxins / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000737:chemistry*; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared
D001427:Bacterial Toxins