[Expression of amino terminal of nucleoprotein and glycoprotein G2 of Hantaan virus in insect cells in the form of fusion protein].
OBJECTIVE
To express the glycoprotein G2 and amino terminal of nucleoprotein (NP) of Hantaan virus in Bac-to-Bac baculovirus expression system in the form of fusion protein.


METHODS
The recombinant baculovirus expression vector pFBDHTa-G2S 0.7 was constructed. The chimeric gene was inserted into bacmid in E.coli DH10Bac with the help of Tn7 transposition system. Then the recombinant baculovirus was screened and the fusion protein was expressed in insect cells. The expression product was detected by ELISA, immunofluorescence assay and Western blot analysis.


RESULTS
The recombinant baculovirus containing the chimeric gene G2S 0.7 had been constructed successfully and the fusion protein could be expressed in insect cells. The expressed protein could be recognized by the Hantaan virus NP-specific mAb and glycoprotein G2-specific mAb.


CONCLUSIONS
The successful expression of fusion protein G2S 0.7 with biological activity in insect cells lays the foundation for further research on its immunological characteristic.
Liu
Yong
Y
Department of Microbiology, Fourth Military Medical University, Xi'an 710032, China.
Xu
Zhi-Kai
ZK
Zhang
Fang-Lin
FL
Luo
Wen
W
Wu
Xing-An
XA
Bai
Wen-Tao
WT
Hu
Gang
G
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D018063:Hantaan virus; D007313:Insecta; D009698:Nucleoproteins / Q000235:genetics
D009698:Nucleoproteins