title: Evaluation of a recombinant salivary gland protein (thrombostasin) as a vaccine candidate to disrupt blood-feeding by horn flies.
abstract: The potential for controlling blood-feeding by the cattle pest, Haematobia irritans irritans (horn fly), was tested by vaccination against thrombostasin (TS), an inhibitor of mammalian thrombin that is released into skin during horn fly blood-feeding. The increase in blood meal size that occurred for flies feeding on sensitized non-vaccinated hosts was blocked and egg development in female flies was delayed when horn flies fed on rabbits and cattle immunized with recombinant TS. This demonstration of the impact of disrupting TS action by vaccination provides a novel approach toward control of this veterinary pest and offers a paradigm for limiting blood-feeding in other medically-important insect species.
authors.affiliation: Department of Entomology and Plant Pathology, 301 Funchess Hall, Auburn University, Auburn, AL 36849, USA. mcupp@acesag.auburn.edu
authors.forename: Mary S
authors.initials: MS
authors.lastname: Cupp
authors.forename: E W
authors.initials: EW
authors.lastname: Cupp
authors.forename: Christine
authors.initials: C
authors.lastname: Navarre
authors.forename: Nancy
authors.initials: N
authors.lastname: Wisnewski
authors.forename: Kevin S
authors.initials: KS
authors.lastname: Brandt
authors.forename: Gary M
authors.initials: GM
authors.lastname: Silver
authors.forename: Dunhua
authors.initials: D
authors.lastname: Zhang
authors.forename: Victor
authors.initials: V
authors.lastname: Panangala
mesh_terms: D000818:Animals; D001769:Blood; D001777:Blood Coagulation; D002417:Cattle; D005247:Feeding Behavior; D007074:Immunoglobulin G / Q000276:immunology; D007300:Insect Control / Q000379:methods*; D019476:Insect Proteins / Q000235:genetics / Q000276:immunology*; D009117:Muscidae / Q000254:growth & development / Q000276:immunology* / Q000502:physiology*; D011817:Rabbits; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D019476:Insect Proteins; C439004:Thrombostasin protein, Haematobia irritans; D014614:Vaccines, Synthetic