title: Growth of Piscirickettsia salmonis to high titers in insect tissue culture cells.
abstract: Piscirickettsia salmonis was grown in established insect, frog, and fish tissue culture cells. The yield of P. salmonis in Sf21 cells was up to 100 times that obtained in CHSE-214 cells, and virulence for Atlantic salmon was retained. The ceiling temperature for growth of P. salmonis in Sf21 cells was 24 degrees C.
authors.affiliation: Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom. T.H.Birkbeck@bio.gla.ac.uk
authors.forename: T Harry
authors.initials: TH
authors.lastname: Birkbeck
authors.forename: Angela A
authors.initials: AA
authors.lastname: Griffen
authors.forename: Helen I
authors.initials: HI
authors.lastname: Reid
authors.forename: L Anthony
authors.initials: LA
authors.lastname: Laidler
authors.forename: Simon
authors.initials: S
authors.lastname: Wadsworth
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D002478:Cells, Cultured; D005393:Fish Diseases / Q000382:microbiology / Q000401:mortality; D044147:Piscirickettsiaceae / Q000254:growth & development* / Q000472:pathogenicity; D044225:Piscirickettsiaceae Infections / Q000382:microbiology / Q000662:veterinary; D020317:Salmo salar / Q000382:microbiology*; D018411:Spodoptera / Q000382:microbiology*; D014774:Virulence