[Preparation and preliminary identification of the monoclonal antibody to bovine bFGF].
OBJECTIVE
To prepare mAb against bovine bFGF and identify their Ig subgroups so as to establish an ELISA for detection of bFGF level.


METHODS
BALB/c mice were immunized by recombinant bovine bFGF. Hybridoma cell lines which could stably secret the monoclonal antibodies to bFGF were established by cell fusion technique, and their related characteristics were identified. In addition, polyclonal antibodies to bFGF were prepared by immunization of rabbits with bovine bFGF. The mAb and polyclonal antibodies purified through protein A affinity chromatography were used to develop a sandwich ELISA for detection of bFGF level.


RESULTS
Three hybridoma cell lines which could secret the mAbs IgG 1 to bFGF were obtained. The concentration of bFGF could be detected by sandwich ELISA developed with purified mAb and polyclonal antibody at nanogram level.


CONCLUSIONS
mAb and polyclonal antibodies against to bovine bFGF have been prepared successfully, which provide powerful tool for further clinical application and related studies.
Liu
Shi-De
SD
College of Life Science, Shenzhen University, Shenzhen 518060, China.
Zhang
Jian-Hua
JH
Qin
Zhi-Hong
ZH
Yu
Yu-Wen
YW
Xing
Miao
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal