[Effect of ligustrazine on the expression of LFA-1 and ICAM-1 in bone marrow transplantation recipient mice].
The objective of study was to investigate the effect of ligustrazine on the expression of LFA-1 and ICAM-1 in bone marrow and the mechanism of hematopoietic reconstitution after bone marrow transplantation (BMT). The 150 mice were randomly divided into 3 groups: normal group, saline group and ligustrazine group. The normal group was not treated. The saline group was given normal saline (0.2 ml/mouse, twice a day) through gastric tube, while the ligustrazine group was given ligustrazine (0.2 ml/mouse, twice a day) through gastric tube. At days 7, 14, 21 and 28 after BMT, the survival rate, colony forming unit of spleen (CFU-S), peripheral blood cells, bone marrow mononuclear cells (BMMNC), histologic changes of bone marrow, as well as the expression level of LFA-1 and ICAM-1 were observed. The results showed that in ligustrazine group CFU-S counts on day 10 and survival rate, WBC and Plt amount in peripheral blood, BMMNC counts, hematopoietic tissue volume as well as the expression level of LFA-1 at 7, 14, 21, 28 days after BMT were higher than in saline group (P < 0.01 or P < 0.05). However, mature RBC volume and expression level of ICAM-1 were lower than in the saline group (P < 0.01 or P < 0.05). Furthermore, fat tissue volume was higher at 7 and 14 days (P < 0.01) and was lower at 21 and 28 days (P < 0.01) after BMT than in the saline group. It is concluded that ligustrazine could improve bone marrow microenvironment and promote hematopoietic reconstitution.
Fu
Li
L
Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Liu
Wen-Li
WL
Sun
Han-Ying
HY
Luo
Lin
L
Zhou
Jian-Feng
JF
Liu
Dan
D
Xu
Hui-Zhen
HZ
D000818:Animals; D001772:Blood Cell Count; D001856:Bone Marrow Examination; D016026:Bone Marrow Transplantation; D005260:Female; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011719:Pyrazines / Q000494:pharmacology*
D016169:Lymphocyte Function-Associated Antigen-1; D011719:Pyrazines; D018799:Intercellular Adhesion Molecule-1; C017953:tetramethylpyrazine