Bright fluorescence of a novel protein from Vibrio vulnificus depends on NADPH and the expression of this protein is regulated by a LysR-type regulatory gene.
A blue fluorescent protein, BfgV, belonging to the short-chain dehydrogenase/reductase superfamily, was found in a non-bioluminescent pathogen Vibrio vulnificus CKM-1. This protein has fluorescence spectra with two excitation peaks at 283 and 352 nm, and one emission peak at 456 nm. BfgV fluoresces through effectively augmenting the intrinsic fluorescence of NADPH bound to it. Escherichia coli transformants expressing this protein can emit eye-detectable fluorescence. A LysR-type transcriptional regulator gene bfgR was found at the vicinal upstream region of bfgV in CKM-1 genome. The clues that products of bfgR can specifically bind to bfgR-bfgV intergenic promoter region and the deletion of bfgR significantly decreases the expression of bfgV reveal bfgR is a repressor gene of bfgV in V. vulnificus CKM-1.
Chang
Chun Chin
CC
Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC.
Chuang
Yin Ching
YC
Chen
Yu Chung
YC
Chang
Ming Chung
MC
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism; D001483:Base Sequence; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry; D005786:Gene Expression Regulation; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000379:methods*; D008969:Molecular Sequence Data; D009249:NADP / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods; D013053:Spectrophotometry; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism; D041261:Vibrio vulnificus / Q000378:metabolism*
D001426:Bacterial Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D009249:NADP; C073470:LysR protein, Bacteria; D004247:DNA