Using a customized DNA microarray for expression profiling of the estrogen-responsive genes to evaluate estrogen activity among natural estrogens and industrial chemicals.
We developed a DNA microarray to evaluate the estrogen activity of natural estrogens and industrial chemicals. Using MCF-7 cells, we conducted a comprehensive analysis of estrogen-responsive genes among approximately 20,000 human genes. On the basis of reproducible and reliable responses of the genes to estrogen, we selected 172 genes to be used for developing a customized DNA microarray. Using this DNA microarray, we examined estrogen activity among natural estrogens (17beta-estradiol, estriol, estrone, genistein), industrial chemicals (diethylstilbestrol, bisphenol A, nonylphenol, methoxychlor), and dioxin. We obtained results identical to those for other bioassays that are used for detecting estrogen activity. On the basis of statistical correlations analysis, these bioassays have shown more sensitivity for dioxin and methoxychlor.
Terasaka
Shunichi
S
Research Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
Aita
Yukie
Y
Inoue
Akio
A
Hayashi
Shinichi
S
Nishigaki
Michiko
M
Aoyagi
Kazuhiko
K
Sasaki
Hiroki
H
Wada-Kiyama
Yuko
Y
Sakuma
Yasuo
Y
Akaba
Shuichi
S
Tanaka
Junko
J
Sone
Hideko
H
Yonemoto
Junzo
J
Tanji
Masao
M
Kiyama
Ryoiti
R
D001681:Biological Assay / Q000379:methods; D001943:Breast Neoplasms / Q000473:pathology; D004785:Environmental Pollutants / Q000494:pharmacology / Q000633:toxicity*; D004967:Estrogens / Q000494:pharmacology / Q000633:toxicity*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D011960:Receptors, Estrogen / Q000187:drug effects*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D004785:Environmental Pollutants; D004967:Estrogens; D011960:Receptors, Estrogen