title: [The effect of artificial support system on serum cytokine in chronic severe hepatitis].
abstract: OBJECTIVE
  To explore the changes of cytokines including TNFalpha, TGFbeta1 and nitrogen monoxide, and endotoxin in the serum of chronic severe hepatitis after the treatment of ALSS, and to evaluate further the value of ALSS in the treatment of chronic severe hepatitis.
  
  
  METHODS
  Forty two patients were screened. The changes of TNFalpha, TGFbeta1, nitrogen monoxide and endotoxin were detected respectively. The relationship between the cytokines and the severity and prognosis were further analyzed.
  
  
  RESULTS
  ALSS was effective to decrease the serum concentration of cytokines. TNFalpha dropped from (481.57+/-229.33) pg/ml to (156.46+/-78.12) pg/ml (P < 0.05). TGFbeta1 from (44.09+/-31.73) ng/ml to (27.77+/-23.28) ng/ml (P < 0.01), endotoxin from (1.05+/-0.37) Eu/ml to (0.28+/-0.22) Eu/ml (P < 0.001). NO from (71.15+/-33.09) micromol/L to (58.11+/-29.30) micromol/L (P < 0.001). Before the therapy endotoxin was related with TNFalpha and total bilirubin, while after the therapy, NO was related with protime and aminonemia.
  
  
  CONCLUSIONS
  High level of endotoxin and nitrogen monoxide in serum plays an important role in hepatocyte damage of chronic severe hepatitis. The changes of serum endotoxin TNFalpha, TGFbeta1 and nitrogen monoxide level in patients with chronic severe hepatitis can be used to judge the severity and prognosis of severe hepatitis. ALSS is a reliable hepatic support device for chronic severe hepatitis
authors.affiliation: Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Qin
authors.forename: Chun-hua
authors.initials: CH
authors.lastname: Qie
authors.forename: Da-zhi
authors.initials: DZ
authors.lastname: Zhang
authors.forename: You-rong
authors.initials: YR
authors.lastname: Zhao
authors.forename: Shu-hua
authors.initials: SH
authors.lastname: Guo
authors.forename: Zhi-yi
authors.initials: ZY
authors.lastname: Wang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000368:Aged; D016207:Cytokines / Q000097:blood*; D004731:Endotoxins / Q000097:blood; D005260:Female; D006521:Hepatitis, Chronic / Q000097:blood / Q000276:immunology / Q000628:therapy*; D006801:Humans; D019164:Liver, Artificial; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood; D011379:Prognosis; D016212:Transforming Growth Factor beta / Q000097:blood
chemical_list: D016207:Cytokines; D004731:Endotoxins; D016212:Transforming Growth Factor beta; D009569:Nitric Oxide