Prevention of cardiac hypertrophy by angiotensin II type-2 receptor gene transfer.
The role of the angiotensin II type-2 receptor (AT2R) in cardiac hypertrophy remains elusive despite its demonstrated involvement in cardiovascular development. We have previously shown that a lentiviral vector gene delivery system is able to transduce cardiac tissue with high efficiency in vivo. Using such an approach, our objectives in the present study were 2-fold: (1) to overexpress the AT2R in cardiac tissue after completion of natural embryonic development of the heart and (2) to determine the effects of this overexpression on cardiac hypertrophy and basal blood pressure (BP). A lentiviral vector encoding the AT2R (lenti-AT2R) was administered (1.5x10(8) transducing units) into the left ventricular space of 5-day-old spontaneously hypertensive rats (SHRs). AT2R transgene expression increased in these animals and persisted for 30 weeks. In contrast, the expression of the angiotensin II type-1 receptor remained unchanged following lenti-AT2R treatment. At 21 weeks following gene transduction, the lenti-AT2R-treated SHRs exhibited decreased left ventricular wall thickness compared with control animals. In contrast, basal BP did not differ between the two SHR groups. Finally, heart weight to body weight ratios indicated a significant decrease in lenti-AT2R-treated SHRs compared with SHR controls. Our data indicate that AT2R overexpression attenuates cardiac hypertrophy in the SHR. This beneficial outcome was observed despite the existence of elevated BP.
Metcalfe
Beverly L
BL
Department of Physiology College of Medicine and the Evelyn F. and William L. McKnight Brain Institute, University of Florida, Gainesville, FL 32610, USA.
Huentelman
Matthew J
MJ
Parilak
Leonard D
LD
Taylor
David G
DG
Katovich
Michael J
MJ
Knot
Harm J
HJ
Sumners
Colin
C
Raizada
Mohan K
MK
D000818:Animals; D000831:Animals, Newborn; D016466:CHO Cells / Q000378:metabolism; D006332:Cardiomegaly / Q000235:genetics / Q000517:prevention & control*; D002478:Cells, Cultured / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D005822:Genetic Vectors / Q000008:administration & dosage; D006321:Heart / Q000254:growth & development; D006352:Heart Ventricles; D006973:Hypertension / Q000235:genetics; D017379:Hypertrophy, Left Ventricular / Q000235:genetics / Q000473:pathology / Q000517:prevention & control; D007267:Injections; D016086:Lentivirus / Q000235:genetics; D032383:Myocytes, Cardiac / Q000378:metabolism; D009929:Organ Size; D051381:Rats; D011918:Rats, Inbred SHR; D017207:Rats, Sprague-Dawley; D044140:Receptor, Angiotensin, Type 1 / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D044139:Receptor, Angiotensin, Type 2 / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000502:physiology
D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2; D011993:Recombinant Fusion Proteins