Isolation of flavohemoglobin from the actinomycete Streptomyces antibioticus grown without external nitric oxide stress.
A flavocytochrome protein was isolated from the actinomycete Streptomyces antibioticus. The purified protein contained protoheme and FAD, and its M(r) was estimated to be 52000. The absorption spectra in its resting oxidized, dithionite-reduced, carbon monoxide-bound, and oxygenated (O(2)-bound) forms were characteristic of those of flavohemoglobin (Fhb). The N-terminal amino acid sequence showed high identities to those of other Fhb's. Furthermore, the actinomycete flavocytochrome scavenged nitric oxide in the presence of NADH. These results demonstrated that the flavocytochrome is the first Fhb purified from actinomycetes. The actinomycete Fhb was produced in S. antibioticus cells in large amounts without any external nitric oxide (NO) stress, which is indicative of a physiological function of Fhb other than detoxification of NO.
Sasaki
Yasuyuki
Y
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Takaya
Naoki
N
Nakamura
Akira
A
Shoun
Hirofumi
H
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D005420:Flavoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D016166:Free Radical Scavengers / Q000737:chemistry; D006420:Hemeproteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D006454:Hemoglobins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000737:chemistry; D018384:Oxidative Stress / Q000502:physiology*; D016415:Sequence Alignment; D013303:Streptomyces antibioticus / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*
D001426:Bacterial Proteins; D005420:Flavoproteins; D016166:Free Radical Scavengers; D006420:Hemeproteins; D006454:Hemoglobins; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase