title: Competitive inhibition of adherence of enterotoxigenic Escherichia coli, enteropathogenic Escherichia coli and Clostridium difficile to intestinal epithelial cell line Lovo by purified adhesin of Bifidobacterium adolescentis 1027.
abstract: OBJECTIVE
  To observe competitive inhibition of adherence of enterotoxigenic Escherichia coli (ETEC), enteropathogenic Escherichia coli (EPEC) and Clostridium difficile (C. difficile) to intestinal epithelial cell line Lovo by purified adhesin of Bifidobacterium adolescentis 1027 (B. ado 1027).
  
  
  METHODS
  The binding of bacteria to intestinal epithelial cell line Lovo was counted by adhesion assay. The inhibition of adherence of ETEC, EPEC and C. difficile to intestinal epithelial cell line Lovo by purified adhesin of B. ado 1027 was evaluated quantitatively by flow cytometry.
  
  
  RESULTS
  The purified adhesin at the concentration of 10 microg/mL, 20 microg/mL and 30 microg/mL except at 1 microg/mL and 5 microg/mL could inhibit significantly the adhesion of ETEC, EPEC and C. difficile to intestinal epithelial cell line Lovo. Moreover, we observed that a reduction in bacterial adhesion was occurred with increase in the concentration of adhesin, and MFI (Mean fluorescent intensity) was decreased with increase in the concentration of adhesin.
  
  
  CONCLUSIONS
  The purified adhesin of B. ado 1027 can inhibit the adhesion of ETEC, EPEC and C. difficile to intestinal epithelial cell line Lovo in a dose-dependent manner.
authors.affiliation: Chinese PLA Institute of Digestive Disease, Nanfang Hospital, First Military Medical University, Guangzhou 510515, Guangdong Province, China. zdoctor@sohu.com
authors.forename: Shi-Shun
authors.initials: SS
authors.lastname: Zhong
authors.forename: Zhen-Shu
authors.initials: ZS
authors.lastname: Zhang
authors.forename: Ji-De
authors.initials: JD
authors.lastname: Wang
authors.forename: Zhuo-Sheng
authors.initials: ZS
authors.lastname: Lai
authors.forename: Qun-Ying
authors.initials: QY
authors.lastname: Wang
authors.forename: Ling-Jia
authors.initials: LJ
authors.lastname: Pan
authors.forename: Yue-Xin
authors.initials: YX
authors.lastname: Ren
mesh_terms: D018829:Adhesins, Bacterial / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D001644:Bifidobacterium; D002460:Cell Line; D016360:Clostridioides difficile / Q000472:pathogenicity / Q000502:physiology*; D003967:Diarrhea / Q000517:prevention & control; D004761:Enterocolitis, Pseudomembranous / Q000517:prevention & control*; D004926:Escherichia coli / Q000472:pathogenicity / Q000502:physiology*; D004927:Escherichia coli Infections / Q000517:prevention & control*; D005434:Flow Cytometry; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000382:microbiology; D014774:Virulence
chemical_list: D018829:Adhesins, Bacterial