NG2-expressing cells in the subventricular zone are type C-like cells and contribute to interneuron generation in the postnatal hippocampus.
The subventricular zone (SVZ) is a source of neural progenitors throughout brain development. The identification and purification of these progenitors and the analysis of their lineage potential are fundamental issues for future brain repair therapies. We demonstrate that early postnatal NG2-expressing (NG2+) progenitor cells located in the SVZ self-renew in vitro and display phenotypic features of transit-amplifier type C-like multipotent cells. NG2+ cells in the SVZ are highly proliferative and express the epidermal growth factor receptor, the transcription factors Dlx, Mash1, and Olig2, and the Lewis X (LeX) antigen. We show that grafted early postnatal NG2+ cells generate hippocampal GABAergic interneurons that propagate action potentials and receive functional glutamatergic synaptic inputs. Our work identifies Dlx+/Mash1+/LeX+/NG2+/GFAP-negative cells of the SVZ as a new class of postnatal multipotent progenitor cells that may represent a specific cellular reservoir for renewal of postnatal and adult inhibitory interneurons in the hippocampus.
Aguirre
Adan A
AA
Center for Neuroscience Research, Children's Research Institute, Rm. 5345, Children's National Medical Center, 111 Michigan Ave., N.W., Washington, DC 20010, USA.
Chittajallu
Ramesh
R
Belachew
Shibeshih
S
Gallo
Vittorio
V
D000200:Action Potentials / Q000235:genetics; D000818:Animals; D000831:Animals, Newborn; D000941:Antigens / Q000235:genetics / Q000378:metabolism*; D051792:Basic Helix-Loop-Helix Proteins; D002454:Cell Differentiation / Q000235:genetics*; D002455:Cell Division / Q000235:genetics; D002465:Cell Movement / Q000235:genetics; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000378:metabolism; D007395:Interneurons / Q000166:cytology / Q000378:metabolism*; D020547:Lateral Ventricles / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D016256:Lewis X Antigen / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009433:Neural Inhibition / Q000235:genetics; D000076104:Oligodendrocyte Transcription Factor 2; D011509:Proteoglycans / Q000235:genetics / Q000378:metabolism*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D009435:Synaptic Transmission / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism; D000099216:Chondroitin Sulfate Proteoglycan 4
D000941:Antigens; D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins; D066246:ErbB Receptors; D018398:Homeodomain Proteins; D016256:Lewis X Antigen; D009419:Nerve Tissue Proteins; D000076104:Oligodendrocyte Transcription Factor 2; D011509:Proteoglycans; D014157:Transcription Factors; D005680:gamma-Aminobutyric Acid; D000099216:Chondroitin Sulfate Proteoglycan 4; C499317:Ascl1 protein, mouse; C088334:Distal-less homeobox proteins; C512773:Olig2 protein, mouse