Differential clustering of Mycoplasma mycoides subsp. mycoides SC strains by PCR-REA of the bgl locus.
In order to develop a specific tool differentiating the African field strains of Mycoplasma mycoides subsp. mycoides SC from other potentially less virulent strains, including the vaccine strains, we have developed a PCR followed by a restriction enzyme analysis (PCR-REA). This approach also differentiates the African field strains from the Australian strains and the type strain PG1. The genomic marker detected by the PCR-REA is based on a single nucleotide change in the bgl gene that codes for 6-phospho-beta-glucosidase (Bgl), an enzyme that is involved in sugar metabolism.
Vilei
Edy M
EM
Department of Research, Institute of Veterinary Bacteriology, University of Bern, Postfach, CH-3001 Bern, Switzerland. edy.vilei@vbi.unibe.ch
Frey
Joachim
J
D000349:Africa; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005819:Genetic Markers; D005959:Glucosidases / Q000737:chemistry / Q000235:genetics; D009176:Mycoplasma mycoides / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D016133:Polymerase Chain Reaction / Q000662:veterinary; D014774:Virulence
D004269:DNA, Bacterial; D005819:Genetic Markers; C056436:endodeoxyribonuclease SpeI; D015252:Deoxyribonucleases, Type II Site-Specific; D005959:Glucosidases; C066586:6-phospho-beta-glucosidase