Substitution of 3'-phosphate cap with a carbon-based blocker reduces the possibility of fluorescence resonance energy transfer probe failure in real-time PCR assays.
Cradic
Kendall W
KW
Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.
Wells
Jason E
JE
Allen
Lindsay
L
Kruckeberg
Kent E
KE
Singh
Ravinder J
RJ
Grebe
Stefan K G
SK
D002244:Carbon; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D010755:Organophosphates / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D010755:Organophosphates; D011933:Reagent Kits, Diagnostic; D002244:Carbon