[Effect of guizhi decoction on CD4+, CD8+ T-lymphocytes and SIgA in mucosal immune system in intestine of mice with Bi syndrome (collagen induced immune arthritis)].
OBJECTIVE
To observe the effect of Guizhi Decoction (GZD) on immunological indexes in intestinal mucosal immune system of mice with Bi syndrome (collagen induced immune arthritis).


METHODS
Eighty male DBA mice were randomly divided into 4 groups, the normal group, the model group and the high and low dosage GZD groups. Model of Bi syndrome was induced by collagen type II. CD4+, CD8+ T-lymphocytes and SIgA in mice' small intestine and PP node were labeled by immunohistochemical staining and their number was calculated using imaging analyzer.


RESULTS
Compared with the normal group, in the model group, after immunization with collagen type II, numbers of CD4+ and CD8+ T-lymphocytes and SIgA in mice' intestinal mucosal immune system reduced significantly (P < 0.05 or P < 0.01). After treatment with GZD of both high and low dosage, CD4+ T-lymphocytes and SIgA increased significantly, but significant difference only showed in comparison between the high dosage GZD group and the model group (P < 0.05 or P < 0.01).


CONCLUSIONS
GZD could enhance the immune function in intestinal mucosa in mice with Bi syndrome, thus it might induce immune tolerance and immune inhibition.
Zhou
Gui-qin
GQ
Department of Pathology, Institute of Basic Theory, China Academy of Traditional Chinese Medicine, Beijing 100700.
Xiao
Cheng
C
Zhou
Jing
J
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D001169:Arthritis, Experimental / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D018928:Immunity, Mucosal; D007071:Immunoglobulin A, Secretory / Q000276:immunology*; D007413:Intestinal Mucosa / Q000276:immunology; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D008517:Phytotherapy; D011897:Random Allocation
D000276:Adjuvants, Immunologic; D004365:Drugs, Chinese Herbal; D007071:Immunoglobulin A, Secretory