title: Detection of 14 alleles derived from the MHC class I A locus in cynomolgus monkeys.
abstract: A basic understanding of the major histocompatibility complex (MHC) class I, which, together with T-cell receptors, is a key player in antigen recognition by cytotoxic T lymphocytes, is necessary to study the cellular immune response to intracellular pathogens. The MHC has hardly been reported in cynomolgus monkeys ( Macaca facicularis), although cynomolgus monkeys have been frequently used as the surrogate animal model. We attempted to determine the nucleotide sequences of the MHC class I A locus of cynomolgus monkeys ( Mafa-A) and eventually 34 independent sequences of Mafa-A were obtained from 29 cynomolgus monkeys. These 34 sequences were classified into 14 Mafa-A alleles according to the results of phylogenetic analyses using the neighbor-joining method. One to three Mafa-A alleles were obtained from a single animal. We also tried to establish a multiplex PCR-SSP method for convenient typing of Mafa-A alleles. cDNA from a family of cynomolgus monkeys, which is composed of four sirs and four dams, were examined by multiplex PCR-SSP. The result of multiplex PCR-SSP showed that an individual cynomolgus monkey had two or three Mafa-A alleles, suggesting that the A locus of cynomolgus monkeys might be duplicated.
authors.affiliation: Tsukuba Primate Center for Medical Science, National Institute of Infectious Diseases, 1 Hachimandai, Tsukuba, 305-0843, Ibaraki, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Uda
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Tanabayashi
authors.forename: Yasuko K
authors.initials: YK
authors.lastname: Yamada
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Akari
authors.forename: Young-Jung
authors.initials: YJ
authors.lastname: Lee
authors.forename: Ryozaburo
authors.initials: R
authors.lastname: Mukai
authors.forename: Keiji
authors.initials: K
authors.lastname: Terao
authors.forename: Akio
authors.initials: A
authors.lastname: Yamada
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005260:Female; D005805:Genes, MHC Class I; D015395:Histocompatibility Antigens Class I / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008252:Macaca fascicularis / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D015395:Histocompatibility Antigens Class I