Real-time quantification of TEL-AML1 fusion transcripts for MRD detection in relapsed childhood acute lymphoblastic leukaemia. Comparison with antigen receptor-based MRD quantification methods.
Response to therapy of ALL assessed by molecular methods has been proved to be a predictor of outcome. Alternatively to established but very labour-intensive DNA-based PCR-techniques the TEL-AML1 fusion transcript can serve as a marker for MRD monitoring. MRD quantification using TEL-AML1 is of particular interest if the results are directly comparable to data obtained by established DNA-based assays. Investigation of the potential of MRD monitoring using LightCycler technology for TEL-AML1 real-time quantification and comparison to results from established DNA-based MRD assays revealed corresponding results. Accordingly, TEL-AML1 MRD quantification is a sensitive, specific and rapid method that can supplement clone-specific MRD detection.
Taube
Tillmann
T
Klinik für Pädiatrie mit Schwerpunkt Onkologie/Hämatologie, Otto-Heubner Centrum für Kinder- und Jugendmedizin, Charité, Medizinische Fakultät der Humboldt-Universität zu Berlin, Forschungshaus 37, 3353 Berlin, Germany. tillmann.taube@charite.de
Eckert
Cornelia
C
Körner
Gabriele
G
Henze
Günter
G
Seeger
Karlheinz
K
D001853:Bone Marrow / Q000473:pathology; D002648:Child; D050676:Core Binding Factor Alpha 2 Subunit; D005453:Fluorescence; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods / Q000592:standards; D018365:Neoplasm, Residual / Q000175:diagnosis* / Q000235:genetics; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis*; D011946:Receptors, Antigen / Q000032:analysis; D012008:Recurrence; D012680:Sensitivity and Specificity; D000097764:ETS Translocation Variant 6 Protein
D050676:Core Binding Factor Alpha 2 Subunit; D015514:Oncogene Proteins, Fusion; D012333:RNA, Messenger; D011946:Receptors, Antigen; C102421:TEL-AML1 fusion protein; D000097764:ETS Translocation Variant 6 Protein