title: A histological evaluation of the involvement of Bio-Oss in osteoblastic differentiation and matrix synthesis.
abstract: This study was designed to investigate the responses of bone cells to a deproteinized bovine bone material, Bio-Oss (Geistlich-Pharma, Wolhunsen, Switzerland), which was grafted in artificial bone defects of rat femurs. Standardized bone defects in the cortical bone of the right femurs were grafted with Bio-Oss particles. Narrow penetrations were prepared on the bottom of the cavity, enabling osteogenic cells to migrate from the bone marrow. A defect in the left femur without Bio-Oss was used as a control. The treated femurs were histochemically examined at 1, 3, 5, 7, and 14 days after the operation. At day 1, no osteogenic migration into the cavities occurred in either the control or experimental groups. At day 3, alkaline phosphatase (ALPase) immunohistochemistry showed a migration of the positive cells at the bottom of the cavities of the experimental groups, but not in the control ones. At day 5, new bone formation was recognized at the bottom of the cavity of both groups. In the experimental group, ALPase-positive cells were localized on Bio-Oss and/or on the thin bone matrix that covered this material. The superficial layer of Bio-Oss underlying the newly formed bone exhibited osteocalcin immunoreactivity. Transmission electron microscopy revealed osteoblasts depositing bone matrices--including collagen fibers--on the surface of Bio-Oss. At days 7 and 14, woven bone occupied the previous cavities of both control and experimental groups, accompanied by osteoclasts. Thus, Bio-Oss appears to serve as a scaffold for osteogenic cells as well as to promote osteoblastic differentiation and matrix synthesis.
authors.affiliation: Division of Oral Anatomy, Department of Oral Biological Science, Niigata University Graduate School of Medicine and Dental Sciences, Gakkoucho-Dori, Japan.
authors.forename: Fabricio I
authors.initials: FI
authors.lastname: Tapety
authors.forename: Norio
authors.initials: N
authors.lastname: Amizuka
authors.forename: Katsumi
authors.initials: K
authors.lastname: Uoshima
authors.forename: Shuichi
authors.initials: S
authors.lastname: Nomura
authors.forename: Takeyasu
authors.initials: T
authors.lastname: Maeda
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001853:Bone Marrow / Q000601:surgery; D001857:Bone Matrix / Q000378:metabolism / Q000637:transplantation*; D018786:Bone Substitutes / Q000627:therapeutic use*; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000502:physiology; D003094:Collagen / Q000378:metabolism; D005269:Femur / Q000601:surgery; D008297:Male; D008854:Microscopy, Electron; D008903:Minerals / Q000627:therapeutic use*; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D015675:Osteocalcin / Q000032:analysis; D010010:Osteoclasts / Q000187:drug effects; D010012:Osteogenesis / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: C077540:Bio-Oss; D018786:Bone Substitutes; D008903:Minerals; D015675:Osteocalcin; D003094:Collagen; D000469:Alkaline Phosphatase