Functional relationship between Na/K-ATPase and NMDA-receptors in rat cerebellum granule cells.
Activation of rat cerebellum granule cells by N-methyl-D-aspartate (NMDA, 10(-4)-10(-3) M) results in progressive increase in reactive oxygen species (ROS) and suppression of the ouabain-sensitive part of Na/K-ATPase activity. When Na/K-ATPase was inhibited by high ouabain concentrations (10(-5)-5 x 10(-4) M), an increase in stationary ROS level in neuronal cells was noted, this effect being attenuated by NMDA antagonists, MK-801 and D-AP5. It is concluded that in cerebellum neurons, ouabain-resistant Na/K-ATPase is responsible for suppression of intracellular level of ROS, which, in turn, inhibit ouabain-sensitive Na/K-ATPase.
Boldyrev
A
A
Department of Biochemistry, Faculty of Biology, Lomonosov Moscow State University, Moscow 119992, Russia. aaboldyrev@mail.ru
Bulygina
E
E
Gerassimova
O
O
Lyapina
L
L
Schoner
W
W
D000818:Animals; D002531:Cerebellum / Q000166:cytology* / Q000201:enzymology; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D017382:Reactive Oxygen Species; D016194:Receptors, N-Methyl-D-Aspartate; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase