title: [Exon polymorphism of human RHD gene].
abstract: OBJECTIVE
  To study exon polymorphism of human RHD gene and investigate the genetic mechanism of RhD-negative individuals.
  
  
  METHODS
  PCR using sequence-specific primers (PCR-SSP) was performed on 40 RhD-positive, 120 RhD-negative and 2 weak D blood samples.
  
  
  RESULTS
  All 10 exons could be detected in the 40 RhD-positive and 2 weak D samples. Out of the 120 RhD-negative samples, 28 (23.33%) carried 10 exons, 19 (15.83%) lost most of the 10 exons (with mainly intermediate deletion), and 73 (60.83%) had deletion of all the 10 exons; 19 samples of Del phenotype identified from the 120 RhD-negative samples had all the 10 exons.
  
  
  CONCLUSIONS
  Polymorphism of the exon structure of RHD gene is present in RhD-negative individuals, characterized chiefly by gross deletion, partial deletion and non-deletion.
authors.affiliation: Department of Blood Transfusion, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. zhy@fimmu.com
authors.forename: Hua-you
authors.initials: HY
authors.lastname: Zhou
authors.forename: Jiong-cai
authors.initials: JC
authors.lastname: Lan
authors.forename: Xiao-zhu
authors.initials: XZ
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Fan
authors.forename: Qing-bao
authors.initials: QB
authors.lastname: Meng
authors.forename: Hong-wei
authors.initials: HW
authors.lastname: Gong
mesh_terms: D044466:Asian People; D005091:Exons; D017353:Gene Deletion; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012204:Rh-Hr Blood-Group System / Q000235:genetics*
chemical_list: D012204:Rh-Hr Blood-Group System