title: Involvement of secreted Aspergillus fumigatus proteases in disruption of the actin fiber cytoskeleton and loss of focal adhesion sites in infected A549 lung pneumocytes.
abstract: Aspergillus fumigatus is an opportunistic pathogenic fungus that predominantly infects the respiratory system. Penetration of the lung alveolar epithelium is a key step in the infectious process. The cytoskeleton of alveolar epithelial cells forms the cellular basis for the formation of a physical barrier between the cells and their surroundings. This study focused on the distinct effects of A. fumigatus on the actin cytoskeleton of A549 lung pneumocytes. Of the 3 major classes of cytoskeletal fibers--actin microfilaments, microtubules, and intermediate filaments--only the actin cytoskeleton was found to undergo major structural changes in response to infection, including loss of actin stress fibers, formation of actin aggregates, disruption of focal adhesion sites, and cell blebbing. These changes could be specifically blocked in wild-type strains of A. fumigatus by the addition of antipain, a serine and cysteine protease inhibitor, and were not induced by an alkaline serine protease-deficient strain of A. fumigatus. Antipain also reduced, by approximately 50%, fungal-induced A549 cell detachment from the plates and reduction in viability. Our findings suggest that A. fumigatus breaches the alveolar epithelial cell barrier by secreting proteases that act together to disorganize the actin cytoskeleton and destroy cell attachment to the substrate by disrupting focal adhesions.
authors.affiliation: Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Tanya V
authors.initials: TV
authors.lastname: Kogan
authors.forename: Jeries
authors.initials: J
authors.lastname: Jadoun
authors.forename: Leonid
authors.initials: L
authors.lastname: Mittelman
authors.forename: Koret
authors.initials: K
authors.lastname: Hirschberg
authors.forename: Nir
authors.initials: N
authors.lastname: Osherov
mesh_terms: D000199:Actins / Q000378:metabolism*; D000976:Antipain / Q000494:pharmacology; D001228:Aspergillosis / Q000382:microbiology*; D001232:Aspergillus fumigatus / Q000201:enzymology* / Q000502:physiology; D001557:Benzenesulfonates / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology; D045744:Cell Line, Tumor; D003599:Cytoskeleton / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism*; D005562:Formazans / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung / Q000382:microbiology / Q000648:ultrastructure; D018613:Microscopy, Confocal; D008870:Microtubules; D011480:Protease Inhibitors / Q000494:pharmacology; D016596:Vinculin / Q000502:physiology
chemical_list: D000199:Actins; D001557:Benzenesulfonates; D005562:Formazans; D011480:Protease Inhibitors; D016596:Vinculin; D000976:Antipain; C059088:1,5-bis(2-methoxy-4-nitro-5-sulfophenyl)-3-((phenylamino)carbonyl)formazan; C007061:C.I. Fluorescent Brightening Agent 28; D010450:Endopeptidases