Photosynthetic electron transport controls expression of the high light inducible gene in the cyanobacterium Synechococcus elongatus strain PCC 7942.
The hliA gene of Synechococcus, encoding a photoprotective high light inducible polypeptide, is up-regulated by high light (HL) or low intensity blue/UV-A light (BL). hliA expression was found to be up-regulated by KCN in low light (LL) (but not in the dark), and up-regulation in HL, BL, and LL (with KCN) was inhibited by 2,5-dibromo-3-methyl-6-isopropyl-benzoquinone. A working hypothesis is proposed whereby up-regulation is in response to the reduced state of cytochrome b(6)f or a carrier beyond in photosynthesis. Modest up-regulation occurs in LL by treatment with 3-(3,4-dichlorophenyl)-1,1-dimethylurea, but this is related to effects on hliA mRNA stability rather than on transcription.
Salem
Kavitha
K
Department of Biology and the Converging Biotechnology Center, Box 19498, The University of Texas at Arlington, Arlington, TX 76019, USA.
van Waasbergen
Lorraine G
LG
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects*; D019069:Cell Respiration / Q000235:genetics / Q000528:radiation effects*; D000458:Cyanobacteria / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects*; D045346:Cytochrome b6f Complex / Q000378:metabolism; D003991:Dibromothymoquinone / Q000494:pharmacology; D004237:Diuron / Q000494:pharmacology; D004579:Electron Transport / Q000235:genetics / Q000528:radiation effects*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics / Q000528:radiation effects; D008027:Light; D045342:Light-Harvesting Protein Complexes / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects*; D010775:Photic Stimulation; D010788:Photosynthesis / Q000235:genetics / Q000528:radiation effects*; D011190:Potassium Cyanide / Q000494:pharmacology; D020871:RNA Stability / Q000187:drug effects / Q000235:genetics; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D013045:Species Specificity; D015854:Up-Regulation / Q000235:genetics / Q000528:radiation effects
D001426:Bacterial Proteins; D045342:Light-Harvesting Protein Complexes; D012333:RNA, Messenger; C091582:high light-inducible protein, cyanobacteria; D003991:Dibromothymoquinone; D045346:Cytochrome b6f Complex; D004237:Diuron; D011190:Potassium Cyanide