Adaptive responses to static conditions in nutrient-rich cultures of luminous Ralstonia eutropha.
The lux-gene fused Ralstonia eutropha, when adapting to static conditions, causes stratification of air-exposed and nutrient-rich cultures at above 0.15 mg biomass ml(-1). The O2 respiring biofilm (luminous neuston) phase, along with the dark sub-neustonic suspension phase, develops within 5-60 min. The instability of the biphasic static culture was identified as a reason for occasionally observable oscillatory bioluminescence.
Simkus
R
R
Institute of Biochemistry, Mokslininku 12, LT-08662 Vilnius, Lithuania. simkus@bchi.lt
Meskiene
R
R
Leth
S
S
Csöregi
E
E
D000222:Adaptation, Physiological / Q000502:physiology; D001683:Biological Clocks / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods*; D020118:Cupriavidus necator / Q000502:physiology*; D006706:Homeostasis / Q000502:physiology; D008163:Luminescent Measurements; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism
D008164:Luminescent Proteins; D011994:Recombinant Proteins; D010100:Oxygen