Detection of anticoagulant activities of isolated canine fibrinogen degradation products X, Y, D and E using resonance thrombography.
The objective of the study was to examine the influence of fibrinogen degradation products (FDPs) on the resonance thrombogram (RTG). Different concentrations (up to 5 g/l plasma) of purified canine FDP X, FDP Y, FDP D and FDP E were added to the blood of healthy dogs and the RTG was measured. Of the examined RTG parameters [reaction time, fibrin formation time (RTG-f), and fibrin amplitude], FDP X, FDP Y, and FDP D showed the clearest effects on RTG-f. RTG-f was on the average prolonged by addition of 0.06 g/l FDP Y, 0.15 g/l FDP X, and 0.17 g/l FDP D to the upper limit of the reference range. Based on the gram amounts of the added FDP, fragment Y also showed in other RTG parameters the most marked effect, followed by FDP X and FDP D. FDP E showed no substantial effect rS [Spearmans rank correlation coefficient (< 0.3, P > or = 0.5)] in any of the examined RTG parameters. The closest correlation between FDP concentration and the result of the different RTG parameters was found for the RTG-f (FDP X, rS = 0.819; FDP Y, rS = 0.795; FDP D, rS = 0.764). The results indicate that RTG is a useful screening test for the detection of increased FDP concentrations.
Mischke
Reinhard
R
Small Animal Clinic, Hannover School of Veterinary Medicine, Hannover, Germany. reinhard.mischke@tiho-hannover.de
Wolling
Hermann
H
Nolte
Ingo
I
D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D001780:Blood Coagulation Tests / Q000379:methods; D004285:Dogs; D005338:Fibrin Fibrinogen Degradation Products / Q000302:isolation & purification* / Q000494:pharmacology*
D005338:Fibrin Fibrinogen Degradation Products