title: Chromosome aberrations of clonal origin are present in astronauts' blood lymphocytes.
abstract: Radiation-induced chromosome translocations remain in peripheral blood cells over many years, and can potentially be used to measure retrospective doses or prolonged low-dose rate exposures. However, several recent studies have indicated that some individuals possess clones of cells with balanced chromosome abnormalities, which can result in an overestimation of damage and, therefore, influence the accuracy of dose calculations. We carefully examined the patterns of chromosome damage found in the blood lymphocytes of twelve astronauts, and also applied statistical methods to screen for the presence of potential clones. Cells with clonal aberrations were identified in three of the twelve individuals. These clonal cells were present in samples collected both before and after space flight, and yields are higher than previously reported for healthy individuals in this age range (40-52 years of age). The frequency of clonal damage appears to be even greater in chromosomes prematurely condensed in interphase, when compared with equivalent analysis in metaphase cells. The individuals with clonal aberrations were followed-up over several months and the yields of all clones decreased during this period. Since clonal aberrations may be associated with increased risk of tumorigenesis, it is important to accurately identify cells containing clonal rearrangements for risk assessment as well as biodosimetry.
authors.affiliation: Wyle Laboratories, Houston, TX 77058, USA. kerry.a.george1@jsc.nasa.gov
authors.forename: K
authors.initials: K
authors.lastname: George
authors.forename: M
authors.initials: M
authors.lastname: Durante
authors.forename: V
authors.initials: V
authors.lastname: Willingham
authors.forename: F A
authors.initials: FA
authors.lastname: Cucinotta
mesh_terms: D000328:Adult; D018480:Astronauts; D002869:Chromosome Aberrations; D019457:Chromosome Breakage; D002877:Chromosomes, Human / Q000528:radiation effects / Q000648:ultrastructure*; D002999:Clone Cells / Q000648:ultrastructure; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000528:radiation effects / Q000648:ultrastructure*; D008875:Middle Aged; D016273:Occupational Exposure; D013026:Space Flight; D014178:Translocation, Genetic