title: [Experiment and study of endothelialization of prostheses with bone marrow CD34+ cells].
abstract: OBJECTIVE
  To exploration the endothelialization of prostheses with bone marrow CD(34)(+) cells.
  
  
  METHODS
  CD(34)(+) cells were isolated from bone marrow of carine by an immune magnetic cell sorting system and induced into endothelial cells with VEGF. Seeding the cells to PTFE prostheses which implanted the abdominal aorta artery (AAA) and inferior vena cava (IVC).
  
  
  RESULTS
  The isolated cells from bone marrow were CD(34)(+) by flow cytometer which could differentiate into endothelial cells in vascular endothelial growth factor (VEGF). The endothelial cells were identified by immunostaining and transmission electron microscope. The obliteration rate of the seeding grafts implanting AAA was 0%, the stenosis rate 12.5%; the obliteration rate implanting IVC 12.5%, the stenosis rate 25%.
  
  
  CONCLUSIONS
  CD(34)(+) cells were isolated from bone marrow by an immune magnetic cell sorting system and were able to be induced into endothelial cells with VEGF in vitro. PTFE prostheses seeding CD(34)(+) cells have the ideal endothelialization and patency.
authors.affiliation: Department of Vascular Surgery, The First Affiliated Hospital College of Medicine, Zhejiang University, HangZhou 310003, China.
authors.forename: Hong-kun
authors.initials: HK
authors.lastname: Zhang
authors.forename: Shu-sen
authors.initials: SS
authors.lastname: Zheng
authors.forename: Zhong-gao
authors.initials: ZG
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.forename: Hua
authors.initials: H
authors.lastname: Feng
authors.forename: Ping
authors.initials: P
authors.lastname: Shan
authors.forename: Hai-ge
authors.initials: HG
authors.lastname: Zhao
authors.forename: Xu-dong
authors.initials: XD
authors.lastname: Chen
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism*; D001807:Blood Vessel Prosthesis; D019917:Blood Vessel Prosthesis Implantation; D001853:Bone Marrow / Q000098:blood supply; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D004285:Dogs; D042783:Endothelial Cells / Q000187:drug effects / Q000648:ultrastructure; D005260:Female; D005434:Flow Cytometry; D008297:Male; D008854:Microscopy, Electron; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology
chemical_list: D018952:Antigens, CD34; D042461:Vascular Endothelial Growth Factor A