Effect of DMSO on radiation-induced chromosome aberrations analysed by FISH.
The purpose of the present work was to determine if the described reduction in the frequency of radiation-induced chromosome aberrations by DMSO is homogeneous within different human chromosomes. Blood samples were irradiated with 4 Gy of X-rays in absence and presence of 0.5 M DMSO. FISH painting was carried out independently for human chromosomes 1, 2, 3, 4, 7, 11 and 12. The observed frequencies of apparently simple translocations and dicentrics for all these chromosomes, showed a homogeneous reduction when the irradiation was done in the presence of DMSO. Moreover, a better fit between the observed and expected frequencies was obtained when (DNA content)2/3 was used to calculate the expected frequencies, instead of just the DNA content. This result supports the idea that for exchange type aberrations, a better adjustment is obtained when the surface area of spherical chromosome territories is considered.
Cigarrán
S
S
Unitat d'Antropologia, Dpt. Biologia Animal, Biologia Vegetal i Ecologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Barrios
L
L
Caballín
M R
MR
Barquinero
J F
JF
D002869:Chromosome Aberrations / Q000187:drug effects*; D002877:Chromosomes, Human / Q000528:radiation effects* / Q000648:ultrastructure; D004249:DNA Damage; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D008297:Male; D011836:Radiation Tolerance / Q000187:drug effects; D014178:Translocation, Genetic
D004121:Dimethyl Sulfoxide