Isolation of granulocytes and mononuclear cells from the blood of dogs, cats, horses and cattle.
A simple discontinuous Percoll density-gradient technique was adapted for isolation of granulocytes and mononuclear cells from cats, dogs, horses and cattle. Separation was accomplished at low speeds using a standard tabletop centrifuge. Cell purity was 100% for both granulocytes and mononuclear cells and cell viability exceeded 95%. Percent recovery of leukocytes ranged from 69 to 83%.
Weiss
D J
DJ
Department of Veterinary Pathobiology College of Veterinary Medicine University of Minnesota St. Paul, Minnesota 55108.
Kraemer
R
R
Schmit
K
K