Surfactant-based methods for prevention of protein adsorption in capillary electrophoresis.
Surfactants such as didodecyldimethyl ammonium bromide (DDAB) and 1,2-dilauroyl-sn-phosphatidylcholine (DLPC) form bilayers at the walls of bare silica capillaries. Once formed, these bilayers are stable in the absence of surfactant in the buffer. DDAB provides a cationic bilayer coating which yields a strong reversed EOF and is effective for separation of cationic proteins. DLPC provides a zwitterionic bilayer coating which is effective for both cationic and anionic proteins. The electroosmotic flow (EOF) is strongly suppressed in DLPC-coated capillaries, thus low mobility proteins are slow to elute, and so the coating is favored for separation of high mobility proteins.
Lucy
Charles A
CA
Department of Chemistry, Gunning-Lemieux Chemistry Centre, University of Alberta, Edmonton, Alberta, Canada.
Baryla
Nicole E
NE
Yeung
Ken K C
KK
D000327:Adsorption; D019075:Electrophoresis, Capillary; D010713:Phosphatidylcholines / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*
D010713:Phosphatidylcholines; D011506:Proteins; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; C046112:didodecyldimethylammonium; C015153:1,2-dilauroylphosphatidylcholine