Reverse genetic analysis of the transcription regulatory sequence of the coronavirus transmissible gastroenteritis virus.
Coronavirus discontinuous transcription uses a highly conserved sequence (CS) in the joining of leader and body RNAs. Using a full-length infectious construct of transmissable gastroenteritis virus, the present study demonstrates that subgenomic transcription is heavily influenced by upstream flanking sequences and supports a mechanism of transcription attenuation that is regulated in part by a larger domain composed of primarily upstream flanking sequences which select appropriately positioned CS elements for synthesis of subgenomic RNAs.
Curtis
Kristopher M
KM
Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7435, USA. rbaric@email.unc.edu
Yount
Boyd
B
Sims
Amy C
AC
Baric
Ralph S
RS
D001483:Base Sequence; D017124:Conserved Sequence; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011995:Recombination, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic; D005760:Transmissible gastroenteritis virus / Q000235:genetics*