title: Anaerobranca californiensis sp. nov., an anaerobic, alkalithermophilic, fermentative bacterium isolated from a hot spring on Mono Lake.
abstract: A novel, obligately anaerobic, alkalithermophilic, chemo-organotrophic bacterium was isolated from the sediment of an alkaline hot spring located on Paoha Island in Mono Lake, California, USA. This rod-shaped bacterium was motile via peritrichous flagella. Isolated strains grew optimally in 5-25 g NaCl l(-1), at pH 9.0-9.5 and at a temperature of 58 degrees C and were fermentative and mainly proteolytic, utilizing peptone, Casamino acids and yeast extract. Optimal growth was seen in the presence of elemental sulfur, polysulfide or thiosulfate with concomitant reduction to hydrogen sulfide. Sulfite was also formed in an equal ratio to sulfide during reduction of thiosulfate. The novel isolate could also reduce Fe(III) and Se(IV) in the presence of organic matter. On the basis of physiological properties, 16S rRNA gene sequence and DNA-DNA hybridization data, strain PAOHA-1(T) (=DSM 14826(T)=UNIQEM 227(T)) belongs to the genus Anaerobranca and represents a novel species, Anaerobranca californiensis sp. nov.
authors.affiliation: Institute of Microbiology, Russian Academy of Science, Moscow 117811, Russia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Gorlenko
authors.affiliation: University of Southern California, Los Angeles, CA 90089, USA.|Jet Propulsion Laboratory/California Institute of Technology, Pasadena, CA 91109-8099, USA.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Tsapin
authors.affiliation: Institute of Microbiology, Russian Academy of Science, Moscow 117811, Russia.
authors.forename: Zorigto
authors.initials: Z
authors.lastname: Namsaraev
authors.affiliation: California Institute of Technology, Pasadena, CA 91125, USA.
authors.forename: Tracy
authors.initials: T
authors.lastname: Teal
authors.affiliation: Institute of Microbiology, Russian Academy of Science, Moscow 117811, Russia.
authors.forename: Tatyana
authors.initials: T
authors.lastname: Tourova
authors.affiliation: Jet Propulsion Laboratory/California Institute of Technology, Pasadena, CA 91109-8099, USA.
authors.forename: Diane
authors.initials: D
authors.lastname: Engler
authors.affiliation: Jet Propulsion Laboratory/California Institute of Technology, Pasadena, CA 91109-8099, USA.
authors.forename: Randy
authors.initials: R
authors.lastname: Mielke
authors.affiliation: University of Southern California, Los Angeles, CA 90089, USA.|Jet Propulsion Laboratory/California Institute of Technology, Pasadena, CA 91109-8099, USA.
authors.forename: Kenneth
authors.initials: K
authors.lastname: Nealson
mesh_terms: D001421:Bacteria, Anaerobic / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002140:California; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005285:Fermentation; D005618:Fresh Water / Q000382:microbiology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013440:Sulfides / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013440:Sulfides