Nerve growth factor (NGF) induces mRNA expression of the new transcription factor protein p48ZnF.
Apoptosis, the cell's intrinsic death program, plays a crucial role in the regulation of tissue homeostasis, and abnormal inhibition of apoptosis is an indicator of cancer and autoimmune diseases, whereas excessive cell death is implicated in neurodegenerative disorders such as Alzheimer's disease (AD). Using cDNA subtraction analysis, we compared p60TRP (p60 transcription regulator protein) expressing cells with control cells during the process of apoptosis and we identified the new zinc-finger protein p48ZnF that is predominantly located in the cytoplasm of the cell. Additionally, we demonstrate here that p48ZnF is up-regulated in rat neuronal PC12 cells upon stimulation with the neurotrophic factor NGF (50 ng/ml). These findings point to a possible pivotal role of p48ZnF in the control of neuronal survival.
Heese
Klaus
K
BF Research Institute Inc., c/o National Cardiovascular Center 5-7-1 Fujishirodai, Suita, Osaka 565-0873, Japan. KHEESE@NTU.EDU.SG
Nagai
Yasuo
Y
Sawada
Tohru
T
D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D001327:Autoimmune Diseases / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D015415:Biomarkers; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects; D003001:Cloning, Molecular; D006224:Cricetinae; D003593:Cytoplasm / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000378:metabolism; D020932:Nerve Growth Factor / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D016716:PC12 Cells; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics*; D014162:Transfection; D016335:Zinc Fingers / Q000235:genetics*
D015415:Biomarkers; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C495885:Zc3h15 protein, rat; D020932:Nerve Growth Factor