title: Crystallization and preliminary X-ray diffraction analysis of an acidic phospholipase A(2) complexed with p-bromophenacyl bromide and alpha-tocopherol inhibitors at 1.9- and 1.45-A resolution.
abstract: An acidic phospholipase A(2) (PLA(2)) isolated from Bothrops jararacussu snake venom was crystallized with two inhibitors: alpha-tocopherol (vitamin E) and p-bromophenacyl bromide (BPB). The crystals diffracted at 1.45- and 1.85-A resolution, respectively, for the complexes with alpha-tocopherol and p-bromophenacyl bromide. The crystals are not isomorphous with those of the native protein, suggesting the inhibitors binding was successful and changes in the quaternary structure may have occurred.
authors.affiliation: Departamento de Física e Biofísica, Instituto de Biociências, UNESP, Dist. Rubiao Jr. s/n-C.P. 510, CEP 18618-000, Botucatu-São Paulo, Brazil.
authors.forename: Agnes A S
authors.initials: AA
authors.lastname: Takeda
authors.forename: Juliana I
authors.initials: JI
authors.lastname: dos Santos
authors.forename: Silvana
authors.initials: S
authors.lastname: Marcussi
authors.forename: Lucas B
authors.initials: LB
authors.lastname: Silveira
authors.forename: Andreimar M
authors.initials: AM
authors.lastname: Soares
authors.forename: Marcos R M
authors.initials: MR
authors.lastname: Fontes
mesh_terms: D000098:Acetophenones / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001665:Binding Sites; D017837:Bothrops; D003435:Crotalid Venoms / Q000737:chemistry / Q000201:enzymology*; D003460:Crystallization; D004791:Enzyme Inhibitors / Q000378:metabolism; D010741:Phospholipases A / Q000737:chemistry* / Q000378:metabolism*; D014810:Vitamin E / Q000378:metabolism*; D014961:X-Ray Diffraction
chemical_list: D000098:Acetophenones; D000975:Antioxidants; D003435:Crotalid Venoms; D004791:Enzyme Inhibitors; D014810:Vitamin E; D010741:Phospholipases A; C006821:4-bromophenacyl bromide