Live cold-adapted influenza A vaccine produced in Vero cell line.
The African green monkey kidney (Vero) cell line was used as a substrate for the development of a live cold-adapted (ca) reassortant influenza vaccine. For that purpose, a new master strain was generated by an adaptation of the wild type (wt) A/Singapore/1/57 virus to growth at 25 degrees C in a Vero cell line. The resulting cold-adapted (ca) muster strain A/Singapore/1/57ca showed temperature sensitive (ts) phenotype and was attenuated in animal models and protective in the challenge experiments in ferrets. Two vaccine candidates of influenza A(H1N1) and A(H3N2) subtypes (6/2 reassortants) inheriting six genes coding internal proteins from the new master strain and the surface antigens hemagglutinin (HA) and neuraminidase (NA) from the epidemic viruses were obtained by a standard method of genetic reassortment. All steps of the vaccine preparation were done exclusively in Vero cells, including the isolation of the epidemic viruses. Both vaccine strains were used for immunization of young adult volunteers in a limited clinical trial and appeared to be safe, well tolerated and immunogenic after intranasal administration.
Romanova
Julia
J
Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18B, A-1190 Vienna, Austria. j.romanova@iam.boku.ac.at
Katinger
Dietmar
D
Ferko
Boris
B
Vcelar
Brigitta
B
Sereinig
Sabine
S
Kuznetsov
Oleg
O
Stukova
Marina
M
Erofeeva
Marjana
M
Kiselev
Oleg
O
Katinger
Hermann
H
Egorov
Andrej
A
D000222:Adaptation, Physiological; D000328:Adult; D000818:Animals; D002522:Chlorocebus aethiops; D003080:Cold Temperature; D005289:Ferrets; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology; D007251:Influenza, Human / Q000517:prevention & control; D051379:Mice; D008807:Mice, Inbred BALB C; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology; D014709:Vero Cells / Q000821:virology; D014779:Virus Replication
D007252:Influenza Vaccines; D014613:Vaccines, Attenuated