[Preparation and identification of polyclonal antibody to B cell epitope domain of TRP-1].
OBJECTIVE
To prepare the polyclonal antibody to B cell epitope domain of TRP-1 and apply it to study the immunotherapy of vitiligo and melanoma.


METHODS
The fusion protein PRSETA/TRP-1 was expressed in E.coli, the polyclonal antibody was prepared by immunizing a rabbit with the protein. The qulity of the antibody was identified by ELISA and Western-blot.


RESULTS
(1)The pRSEA/TRP-1 fusion protein was correctly expressed;(2)the polyclonal antibodies to the B cell eoitope domain of TRP-1 were obtained; (3)the rabbit antiserum containing of polyclonal antibody showed high titer and possessed good binding activity to 6His-TRP1 expressed by Pichia pastoris.


CONCLUSIONS
The polyclonal antibody could be applied to the research on B cell epitope domain of TRP-1.
Li
Ting-Hui
TH
Department of Dermatology and Venerology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Gao
Tian-Wen
TW
Li
Chun-Ying
CY
Hou
Xiao-Bin
XB
Liu
Yu-Feng
YF
D000818:Animals; D000906:Antibodies / Q000276:immunology; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D018985:Epitopes, B-Lymphocyte; D004926:Escherichia coli / Q000378:metabolism; D007106:Immune Sera / Q000276:immunology
D000906:Antibodies; D018985:Epitopes, B-Lymphocyte; D007106:Immune Sera