Crystallization and preliminary crystallographic analysis of Bacillus subtilis guanine deaminase.
Guanine deaminase, a key enzyme in nucleotide metabolism, catalyzes the hydrolytic deamination of guanine to xanthine. The first guanine deaminase crystal from Bacillus subtilis was grown in the absence or presence of the inhibitor hypoxanthine in 30% polyethylene glycol 4000, 0.2 M ammonium acetate and 0.1 M sodium citrate pH 6.5. The crystals belong to space group C222(1), with unit-cell parameters a = 84.91, b = 90.90, c = 80.19 angstroms, with one dimer per asymmetric unit. The crystals diffract X-rays to beyond 1.2 angstroms resolution and an initial atomic model has been built based on selenomethionyl multiwavelength anomalous data at 2 angstroms resolution. Unexpectedly, this is the first domain-swapped structure in the cytidine deaminase superfamily.
Chang
Yu Jui
YJ
Structural Biology Program, National Yang-Ming University, Taipei, Taiwan.
Huang
Chun Hsiang
CH
Hu
Chih Yung
CY
Liaw
Shwu Huey
SH
D000085:Acetates / Q000737:chemistry; D001412:Bacillus subtilis / Q000201:enzymology*; D018360:Crystallography, X-Ray / Q000379:methods*; D019281:Dimerization; D004926:Escherichia coli / Q000378:metabolism; D005583:Fourier Analysis; D006148:Guanine Deaminase / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D019271:Hypoxanthine / Q000737:chemistry; D008958:Models, Molecular; D011092:Polyethylene Glycols / Q000737:chemistry; D011487:Protein Conformation; D012643:Selenium / Q000737:chemistry; D014961:X-Ray Diffraction; D015032:Zinc / Q000737:chemistry
D000085:Acetates; D019271:Hypoxanthine; D011092:Polyethylene Glycols; D006148:Guanine Deaminase; D012643:Selenium; D015032:Zinc; C018824:ammonium acetate