Intraventricular transplantation of embryonic stem cell-derived neural stem cells in intracerebral hemorrhage rats.
In the present study, we attempted to explore cell transplantation therapy for intracerebral hemorrhage (ICH) using embryonic stem (ES) cells. Collagenase-induced ICH rats were used as model animals. Mouse ES cells were differentiated into nestin-positive neural stem cells in vitro by alltrans retinoic acid (ATRA). ATRA-treated ES cells (10(5)) were transplanted into the lateral ventricle in the hemisphere contralateral to the hemorrhage 7 days after collagenase infusion. Twenty-eight days after transplantation, ES-derived neurons and astrocytes were observed around the hematoma cavities of the brain in all of the ten rats receiving grafts. Graft-derived neurons were found in the subependymal area of the lateral ventricle as cellular nodules. Although one of the ten rats receiving grafts showed uncontrolled growth of astroglia derived from the ES cells, intraventricular transplantation of ATRA-treated ES cells is an effective delivery system of neuronal lineage-committed progenitor cells toward the site of ICH.
Nonaka
Masahiro
M
Department of Neurosurgery, Nara Medical University, Kashihara, Japan.
Yoshikawa
Masahide
M
Nishimura
Fumihiko
F
Yokota
Hiroshi
H
Kimura
Hajime
H
Hirabayashi
Hidehiro
H
Nakase
Hiroyuki
H
Ishizaka
Shigeaki
S
Wanaka
Akio
A
Sakaki
Toshisuke
T
D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002455:Cell Division / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D002543:Cerebral Hemorrhage / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D017364:Collagenases / Q000378:metabolism; D004195:Disease Models, Animal; D004622:Embryo, Mammalian / Q000166:cytology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D049452:Green Fluorescent Proteins; D007150:Immunohistochemistry / Q000379:methods; D066298:In Vitro Techniques; D007211:Indoles / Q000378:metabolism; D007276:Injections, Intraventricular; D007381:Intermediate Filament Proteins / Q000378:metabolism; D020547:Lateral Ventricles / Q000378:metabolism; D008164:Luminescent Proteins / Q000378:metabolism; D008297:Male; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D051381:Rats; D011916:Rats, Inbred F344; D013194:Staining and Labeling / Q000379:methods; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000502:physiology*
D005904:Glial Fibrillary Acidic Protein; D007211:Indoles; D007381:Intermediate Filament Proteins; D008164:Luminescent Proteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; C577486:Nes protein, rat; D064231:Nestin; D049452:Green Fluorescent Proteins; C007293:DAPI; D017364:Collagenases