Plasmid-based short-hairpin RNA interference in the chicken embryo.
The chicken neural tube serves as an ideal model for new techniques to alter gene expression. We show that short-hairpin RNA delivered by a plasmid vector can effectively knock down expression of both exogenous and endogenous genes in the chicken neural tube. We also assay the effects of short-hairpin RNA on the interferon response and find no difference between electroporation of a control plasmid and a short-hairpin RNA plasmid.
Chesnutt
Catherine
C
Neuroscience Program, Weill Graduate School of Medical Sciences of Cornell University, New York, New York, USA.
Niswander
Lee
L
D000818:Animals; D002642:Chick Embryo / Q000378:metabolism*; D018274:Electroporation; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D007372:Interferons / Q000494:pharmacology; D060850:LIM-Homeodomain Proteins; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009420:Nervous System / Q000196:embryology*; D010957:Plasmids / Q000235:genetics*; D034622:RNA Interference; D012320:RNA Polymerase III / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors
D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; D009419:Nerve Tissue Proteins; D034741:RNA, Small Interfering; D014157:Transcription Factors; C063843:insulin gene enhancer binding protein Isl-1; D049452:Green Fluorescent Proteins; D007372:Interferons; D012320:RNA Polymerase III