[Expression of a secreted fusion protein consisting of anti-HER2 antibody and reversed caspase-3 and its suppression on growth of SKOV3 cells].
OBJECTIVE
To investigate the killing effect on SKOV3 cells by fusion protein HER2-specific antibody-reversed caspase-3 in the secreted form.


METHODS
The reversed human caspase-3 gene was subcloned into pCMV-e23scFv-PEII-PEIII to construct recombinant eukaryotic expression vector pCMV-e23scFv-PEII-revcasp-3 and transfect Jurkat cells. The secreted expression of the in culture supernatant of transfected Jurkat cells was detected by ELISA. The suppression of the fusion protein on growth of SKOV3 cells was examined by co-culture with supernatant of transfected Jurkat cells.


RESULTS
There was the secreted expression of the fusion protein in Jurkat cells. Expressed product had the activity of inducing SKOV3 cells to death.


CONCLUSIONS
The fusion protein expressed in the secreted form can targeted towards SKOV3 cells and kill them.
Zhang
Li-Hong
LH
Department of Biochemistry and Molecular Biology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi'an 710032, China.
Jia
Lin-Tao
LT
Bao
Wei
W
Yu
Cui-Juan
CJ
Zhao
Jing
J
Jin
Ming
M
Wang
Cheng-Ji
CJ
Yang
An-Gang
AG
D000906:Antibodies / Q000235:genetics; D017209:Apoptosis / Q000235:genetics; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014162:Transfection
D000906:Antibodies; D011993:Recombinant Fusion Proteins; D053148:Caspase 3