title: Chicken ovalbumin upstream promoter-transcription factor I represses the transcriptional activity of the human muscle glycogen phosphorylase promoter in C2C12 cells.
abstract: The responsiveness of the 1.13 kb proximal human muscle glycogen phosphorylase (MGP) gene promoter to the chicken ovalbumin upstream promoter-transcription factor (COUP-TF) repressor, known to be ablated during muscle cell differentiation, was examined. Constitutive expression of COUP-TFI repressed the activity of the promoter in C2C12 muscle cells and sequential deletion analysis mapped the sensitive region between nucleotides -362 and -185, which included a putative consensus COUP-TF binding half-site at -198/-193. Mutation of this site abolished transcriptional response to COUP-TFI of the -362 construct. A -209/-180 probe bound in vitro to COUP-TFI and to protein extracts from proliferating but not fusing myoblasts. Thus, COUP-TF may be involved in repression of the human MGP gene promoter at the myoblast stage.
authors.affiliation: Department de Bioquímica i Biologia Molecular, Facultat de Química, Universitat de Barcelona, Martí i Franquès 1, E-08028 Barcelona, Spain. aferrer@bio.ub.es
authors.forename: Andreu
authors.initials: A
authors.lastname: Ferrer-Martínez
authors.forename: Mario
authors.initials: M
authors.lastname: Marotta
authors.forename: Angel
authors.initials: A
authors.lastname: Baldán
authors.forename: Diego
authors.initials: D
authors.lastname: Haro
authors.forename: Anna M
authors.initials: AM
authors.lastname: Gómez-Foix
mesh_terms: D000818:Animals; D001665:Binding Sites; D051838:COUP Transcription Factor I; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000502:physiology*; D017353:Gene Deletion; D024981:Glycogen Phosphorylase / Q000235:genetics*; D006801:Humans; D051379:Mice; D008957:Models, Genetic; D009132:Muscles / Q000201:enzymology*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011987:Receptors, Steroid / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D051838:COUP Transcription Factor I; D017931:DNA Primers; D004268:DNA-Binding Proteins; C497589:NR2F1 protein, human; C497590:Nr2f1 protein, mouse; D011987:Receptors, Steroid; D014157:Transcription Factors; D024981:Glycogen Phosphorylase