[Effects of leukemia bone marrow stromal cells on resistance of co-cultured HL-60 to idarubicin].
To study the role of hematopoietic microenvironment abnormality in development of minimal residual disease and its mechanism, the viability of HL-60 cells was investigated by means of bone marrow stromal cell culture system or co-culture system of bone marrow stromal cell with HL-60 cells and idarubicin (IDA), flow cytometry and ELISA. The results showed that viability of HL-60 cells gradually decreased along with the increase of IDA dose and prolongation of culture time. Amount of HL-60 cells co-cultured with leukemia bone marrow stramal cells was significantly increased as compared with that of the control (P < 0.05). Bone marrow stromal cells or stromal cell conditioned medium reduced the effect of IDA on HL-60 cells in culture. In conclusion, leukemia bone marrow stromal cells contribute to increasing resistance of HL-60 cells to chemotherapeutic agents, and play some role in developing minimal residual disease.
Zhang
Xi
X
Department of Hematology, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, China.
Wang
Ping
P
Chen
Xing-Hua
XH
Liu
Lin
L
Peng
Xian-Gui
XG
Wang
Qing-Yu
QY
Kong
Pei-Yan
PY
Liu
Hong
H
Zhang
Yi
Y
Gao
Lei
L
Zhong
Yong-Ming
YM
D001854:Bone Marrow Cells / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects; D018920:Coculture Techniques; D004305:Dose-Response Relationship, Drug; D019008:Drug Resistance, Neoplasm; D018922:HL-60 Cells / Q000187:drug effects*; D006801:Humans; D015255:Idarubicin / Q000494:pharmacology*; D017154:Stromal Cells / Q000502:physiology
D015255:Idarubicin