title: Repeated streptozotocin injections cause early onset of glomerulosclerosis in mice.
abstract: Diabetic nephropathy (DN), a major cause of end-stage chronic renal failure, is histologically characterized by glomerulosclerosis. To investigate the molecular mechanisms of DN, it is important to establish a stable model of glomerulosclerosis in mice, because genomic manipulation techniques (such as gene destruction or transgene insertion) are well established in rodent species. In this study, we found that repeated administrations of streptozotocin led to early onset of glomerular sclerotic lesions in C57BL/6 mice, accompanied with renal dysfunction. During the natural course of DN, glomerular endothelial cells decreased at 10 weeks after the start of streptozotocin-injections, whereas myofibroblastic mesangial cells became evident. Our results provide an animal tool to elucidate the molecular mechanisms of DN, for example to investigate vascular pathology in diabetic glomerular diseases.
authors.affiliation: Division of Molecular Regenerative Medicine, Department of Molecular Regenerative Medicine, Osaka University, Suita, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Mizuno
authors.forename: Jinhua
authors.initials: J
authors.lastname: Wen
authors.forename: Yoko
authors.initials: Y
authors.lastname: Mizuno-Horikawa
mesh_terms: D000818:Animals; D003928:Diabetic Nephropathies / Q000139:chemically induced* / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D007150:Immunohistochemistry; D007267:Injections; D051379:Mice; D008810:Mice, Inbred C57BL; D013311:Streptozocin / Q000633:toxicity*; D013997:Time Factors
chemical_list: D013311:Streptozocin