title: Escherichia coli methionine aminopeptidase with Tyr168 to alanine substitution can improve the N-terminal processing of recombinant proteins with valine at the penultimate position.
authors.affiliation: Institute of Molecular Biology, Academia Sinica, NanKang Taipei 11529, Taiwan, ROC.
authors.forename: Li-Fan
authors.initials: LF
authors.lastname: Liu
authors.forename: Cheng-Hsilin
authors.initials: CH
authors.lastname: Hsieh
authors.forename: Pei-Fen
authors.initials: PF
authors.lastname: Liu
authors.forename: Shu-Ping
authors.initials: SP
authors.lastname: Tsai
authors.forename: Ming F
authors.initials: MF
authors.lastname: Tam
mesh_terms: D000409:Alanine / Q000235:genetics / Q000378:metabolism*; D000626:Aminopeptidases / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D063208:Methionyl Aminopeptidases; D011499:Protein Processing, Post-Translational / Q000502:physiology; D013379:Substrate Specificity; D014443:Tyrosine / Q000235:genetics / Q000378:metabolism*; D014633:Valine / Q000378:metabolism*
chemical_list: D014443:Tyrosine; D000626:Aminopeptidases; D063208:Methionyl Aminopeptidases; D014633:Valine; D000409:Alanine