Separation of rutin nona(H-) and deca(H-) sulfonate sodium by ion-pairing reversed-phase liquid chromatography.
Ion-pairing reversed-phase liquid chromatography (RPLC) was used to separate two polysulfonates, rutin nona(H-) sulfonate sodium and rutin deca(H-) sulfonate sodium, which have very similar chemical structures. The final product always contained both of them when one of the compounds was synthesized. Baseline separation was achieved on a C8-bonded silica column at ambient temperature. The eluent was acetonitrile-15 mM phosphate buffer solution containing 20 mM TBA (pH 6.0) (46:54, v/v). The calibration plot was linear in the concentration range 0.5-200 microg ml(-1) for both analytes. The limits of detection (LODs; 254 nm) were 0.03 microg ml(1-) for rutin nona(H-) sulfonate sodium and 0.04 microg ml(-1) for rutin deca(H-) sulfonate sodium. Three batches of rutin deca(H-) sulfonate sodium were analyzed using the assay; the results showed that the analytical performance is really satisfactory.
Wang
Xiang-Jun
XJ
Department of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, 353 Yanan Road, Hangzhou, Zhejiang 310031, PR China.
Tang
Yi-Hong
YH
Yao
Tong-Wei
TW
Zeng
Su
S
D002853:Chromatography, Liquid / Q000379:methods*; D007477:Ions; D007536:Isomerism; D015203:Reproducibility of Results; D012431:Rutin / Q000737:chemistry / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D007477:Ions; D012431:Rutin