Evolution and conservation of microsatellite markers for Leishmania tropica.
Sixteen polymorphic microsatellite markers were developed for phylogenetic analysis of Leishmania tropica. The phylogenetic tests done demonstrated that they do provide a powerful tool for epidemiological studies. They were also tested for their ability to differentiate strains of other species of Leishmania, confirming that microsatellite markers developed for one leishmanial species cannot generally be used for other leishmanial species. In addition to length variation, a high degree of allelic heterozygosity was seen among the strains investigated, suggestive of sexual recombination within the species L. tropica.
Schwenkenbecher
Jan M
JM
Institute of Microbiology and Hygiene, Humboldt University, Charité, Berlin, Germany. janschwenkenbecher@yahoo.de
Fröhlich
Chris
C
Gehre
Florian
F
Schnur
Lionel F
LF
Schönian
Gabriele
G
D000483:Alleles; D000818:Animals; D005075:Biological Evolution; D016054:DNA, Protozoan / Q000032:analysis / Q000302:isolation & purification; D018740:Genetic Heterogeneity; D006579:Heterozygote; D018320:Leishmania major / Q000235:genetics; D007895:Leishmania tropica / Q000235:genetics*; D018895:Microsatellite Repeats; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D016054:DNA, Protozoan