Respiration activity of Escherichia coli entrapped in a cone-shaped microwell and cylindrical micropore monitored by scanning electrochemical microscopy (SECM).
The metabolic activity of E. coli cells embedded in collagen gel microstructures in a cone-shaped well and in a cylindrical micropore was investigated using scanning electrochemical microscopy (SECM), based on the oxygen consumption rate and the conversion rate from ferrocyanide to ferricyanide. The analysis of the concentration profiles for oxygen and ferrocyanide afforded the oxygen consumption rate and the ferrocyanide production rate. A comparison indicated that the ferrocyanide production rates were larger than the oxygen consumption rate, and also that the rates observed in the cylindrical micropore were larger than those observed in the cone-shaped well. The ferrocyanide production rate of a single E. coli cell was calculated to be (5.4 +/- 2.6) x 10(-19) mol s(-1), using a cylindrical micropore system.
Kaya
Takatoshi
T
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai 980-8579, Japan.
Numai
Daisuke
D
Nagamine
Kuniaki
K
Aoyagi
Shigeo
S
Shiku
Hitoshi
H
Matsue
Tomokazu
T
D004563:Electrochemistry / Q000379:methods; D004926:Escherichia coli / Q000502:physiology*; D005295:Ferrocyanides / Q000032:analysis; D008832:Microchemistry / Q000379:methods; D008855:Microscopy, Electron, Scanning; D010100:Oxygen / Q000032:analysis; D012119:Respiration
D005295:Ferrocyanides; C020354:hexacyanoferrate II; D010100:Oxygen