title: Receptor editing is the main mechanism of B cell tolerance toward membrane antigens.
abstract: Self-reactive B cells specific for ubiquitous membrane-bound autoantigens are eliminated in the bone marrow by two mechanisms of tolerance: receptor editing and clonal deletion. However, the relative contributions of clonal deletion and receptor editing to B cell tolerance in a polyclonal B cell population have not been established. Here we show that tolerance toward a membrane antigen-reactive B cell clone acts by receptor editing with very minimal cell loss. The capacity of receptor editing to rescue almost all autoreactive B cells from deletion relies on the availability of multiple joining light chain gene segments as substrate for secondary immunoglobulin light chain gene rearrangement and is independent of the affinity of the autoantigen and the presence of non-autoreactive B cells. Our data further suggest that clonal deletion is a default pathway that functions only when receptor editing has been exhausted.
authors.affiliation: Integrated Department of Immunology, National Jewish Medical and Research Center and University of Colorado Health Sciences Center, 1400 Jackson Street, Denver, Colorado 80206, USA.
authors.forename: Regina
authors.initials: R
authors.lastname: Halverson
authors.forename: Raul M
authors.initials: RM
authors.lastname: Torres
authors.forename: Roberta
authors.initials: R
authors.lastname: Pelanda
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology* / Q000378:metabolism; D001324:Autoantigens / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002455:Cell Division / Q000502:physiology; D002462:Cell Membrane / Q000276:immunology* / Q000378:metabolism; D017637:Clonal Deletion / Q000502:physiology; D007108:Immune Tolerance / Q000276:immunology* / Q000502:physiology; D051379:Mice; D011956:Receptors, Cell Surface / Q000276:immunology* / Q000378:metabolism
chemical_list: D000941:Antigens; D001324:Autoantigens; D011956:Receptors, Cell Surface