High pressure induces scrapie-like prion protein misfolding and amyloid fibril formation.
Our understanding of conformational conversion of proteins in diseases is essential for any diagnostic and therapeutic approach. Although not fully understood, misfolding of the prion protein (PrP) is implicated in the pathogenesis of prion diseases. Despite several efforts to produce the pathologically misfolded conformation in vitro from a recombinant PrP, no positive result has yet been obtained. Within the "protein-only hypothesis", the reason for this hindrance may be that the experimental conditions used did not allow selection of the pathway adopted in vivo resulting in conversion into the infectious form. Here, using a pressure perturbation approach, we show that recombinant PrP is converted to a novel misfolded conformer, which is prone to aggregate and ultimately form amyloid fibrils. A short incubation at high pressure (600 MPa) of the truncated form of hamster prion protein (SHaPrP(90-231)) resulted in the formation of pre-amyloid structures. The mostly globular aggregates were characterized by ThT and ANS binding, and by a beta-sheet-rich secondary structure. After overnight incubation at 600 MPa, amyloid fibrils were formed. In contrast to pre-amyloid structures, they showed birefringency of polarized light after Congo red staining and a strongly decreased ANS binding capacity, but enhanced ThT binding. Both aggregate types were resistant to digestion by PK, and can be considered as potential scrapie-like forms or precursors. These results may be useful for the search for compounds preventing pathogenic PrP misfolding and aggregation.
Torrent
Joan
J
Université de Montpellier 2, EA3763, Place Eugène Bataillon, F-34095 Montpellier cédex 5, France.
Alvarez-Martinez
Maria Teresa
MT
Harricane
Marie-Cécile
MC
Heitz
Frédéric
F
Liautard
Jean-Pierre
JP
Balny
Claude
C
Lange
Reinhard
R
D000682:Amyloid / Q000737:chemistry*; D000817:Anilino Naphthalenesulfonates / Q000494:pharmacology; D000818:Animals; D003224:Congo Red; D006224:Cricetinae; D019286:Endopeptidase K / Q000378:metabolism; D005456:Fluorescent Dyes / Q000494:pharmacology; D011312:Pressure; D017096:Prion Diseases / Q000473:pathology; D011328:Prions / Q000737:chemistry* / Q000648:ultrastructure; D011487:Protein Conformation / Q000187:drug effects; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
D000682:Amyloid; D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D011328:Prions; D011994:Recombinant Proteins; D003224:Congo Red; C027132:1-anilino-8-naphthalenesulfonate; D019286:Endopeptidase K