Gene expression studies using self-fabricated parasite cDNA microarrays.
DNA microarray platforms represent a functional genomics technology that uses structured information obtained from genomic sequencing efforts as a means to study transcriptional processes in a systematic and high-throughput manner. Specifically in this chapter, we outline the ordered processes involved in large-scale parasite gene expression studies including complementary (cDNA) microarray fabrication, total RNA isolation, cDNA labeling using fluorochromes, and DNA:DNA hybridization. Methods described herein were adapted for the study of schistosome sexual maturation and developmental biology but could be easily modified for the study of any additional parasitological system.
Hoffmann
Karl F
KF
Department of Pathology, University of Cambridge, UK.
Fitzpatrick
Jennifer M
JM
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics*; D015870:Gene Expression; D020411:Oligonucleotide Array Sequence Analysis; D010271:Parasites / Q000235:genetics*; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D018076:DNA, Complementary