Tissue distribution and purification of prophenoloxidase in larvae of Asian Corn Borer, Ostrinia furnacalis Guenee (Lepidoptera: Pyralidae).
Using ammonium sulphate precipitation, Blue-Sepharose CL-6B, Phenyl-Sepharose CL-4B, prophenoloxidase (PPO) was isolated and purified from hemolymph of Ostrinia furnacalis larvae. This zymogen was a heterodimer, and composed of two subunits with the relative molecular mass ranging from 66.2 kD to 97.4 kD determined by SDS-PAGE. Western blotting and indirect immunofluorescence test showed that PPO was present in integument, hemolymph plasma and cell membrane of granular hemocytes and oenocytoids of O. furnacalis larvae.
Feng
Cong-Jing
CJ
Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200025, China.
Fu
Wen-Jun
WJ
D000818:Animals; D004156:Catechol Oxidase / Q000378:metabolism*; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000378:metabolism*; D005216:Fat Body / Q000201:enzymology*; D006434:Hemocytes / Q000201:enzymology*; D006458:Hemolymph / Q000201:enzymology*; D007814:Larva / Q000201:enzymology; D007915:Lepidoptera / Q000201:enzymology*; D008970:Molecular Weight; D009928:Organ Specificity; D014018:Tissue Distribution
D004792:Enzyme Precursors; C038451:pro-phenoloxidase; D004156:Catechol Oxidase