Effect of octreotide on expression of calcitonin gene in cultured cells of medullary thyroid carcinoma.
The studies were performed on cultured TT cells, originating from medullary thyroid carcinoma and producing calcitonin (CT) and CT gene-related peptide (CGRP), synthesized on the template of the same gene. Addition of octreotide (a synthetic somatostatin analogue) to the cell culture augmented amounts of CGRP mRNA and of CGRP protein in the cells as well as increased levels of CGRP in the culture medium. In contrast, amounts of CT mRNA and of CT protein in the cells as well as levels of CT in the medium remained unchanged. The results showed that octreotide stimulates transcription and alternate splicing of the CT gene.
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Department of Histology and Embryology, Wroclaw University of Medicine, Wroclaw, Poland. mazab@hist.am.wroc.pl
D017398:Alternative Splicing / Q000187:drug effects; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology*; D002116:Calcitonin / Q000235:genetics / Q000378:metabolism*; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D018276:Carcinoma, Medullary / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D003470:Culture Media; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D015282:Octreotide / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology; D014158:Transcription, Genetic / Q000187:drug effects
D018931:Antineoplastic Agents, Hormonal; D003470:Culture Media; D012333:RNA, Messenger; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide; D015282:Octreotide