title: Elevated expression of matrix metalloproteinase-13 in abdominal aortic aneurysms.
abstract: Destructive remodeling of extracellular matrix has been shown to be present in aneurysmal abdominal aorta. We used real-time quantitative reverse transcriptase polymerase chain reaction to determine the relative expression of matrix metalloproteinase-13 (MMP13) in aortic tissue samples from patients who underwent abdominal aortic aneurysm repair operations (n = 36) and from nonaneurysmal autopsy samples (n = 20). The assays were carried out simultaneously in the same reaction tubes for ribosomal 18S RNA to correct for different amounts of input RNA. MMP13 was expressed in all parts of aorta and its expression was elevated in aneurysmal sac. In further studies using MMP13-specific antibody we demonstrated that MMP13 protein was present in the aneurysmal wall.
authors.affiliation: Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI 48201, USA.
authors.forename: Gerard
authors.initials: G
authors.lastname: Tromp
authors.forename: Zoran
authors.initials: Z
authors.lastname: Gatalica
authors.forename: Magdalena
authors.initials: M
authors.lastname: Skunca
authors.forename: Ramon
authors.initials: R
authors.lastname: Berguer
authors.forename: Thomas
authors.initials: T
authors.lastname: Siegel
authors.forename: Ronald A
authors.initials: RA
authors.lastname: Kline
authors.forename: Helena
authors.initials: H
authors.lastname: Kuivaniemi
mesh_terms: D000368:Aged; D001012:Aorta, Abdominal / Q000201:enzymology; D017544:Aortic Aneurysm, Abdominal / Q000201:enzymology*; D017364:Collagenases / Q000096:biosynthesis* / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D053509:Matrix Metalloproteinase 13; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D017364:Collagenases; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13