An addressable antibody nanoarray produced on a nanostructured surface.
The ability to address specific nanoscale features is required to produce diverse biological nanoarrays or perform local assembly using biological building blocks and is an important unsolved problem in nanotechnology. In this work, we describe the use of a novel nanofabricated gold surface, with regions of distinct topography and chemical functionalities, to solve this problem. First, nanoarrays of IgG antibodies were produced by selective immobilization in nanoholes on the surface. The smallest feature size was determined by the hole size (fwhm 90 nm) and not surface diffusion. Using holes of 300 nm diameter, we selectively addressed specific features in the array by nanopipet delivery of a functional antibody, anti-IgG. To our knowledge, this is the first example of addressing specific biologically functional features on a surface at the nanoscale.
Bruckbauer
Andreas
A
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.
Zhou
Dejian
D
Kang
Dae-Joon
DJ
Korchev
Yuri E
YE
Abell
Chris
C
Klenerman
David
D
D015097:3-Mercaptopropionic Acid / Q000737:chemistry; D000327:Adsorption; D000906:Antibodies / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D019855:Ethylene Glycol / Q000737:chemistry; D005708:Gallium / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D007074:Immunoglobulin G / Q000276:immunology; D008856:Microscopy, Fluorescence / Q000379:methods; D036103:Nanotechnology / Q000379:methods*; D013499:Surface Properties
D000906:Antibodies; D007074:Immunoglobulin G; D006046:Gold; D015097:3-Mercaptopropionic Acid; D005708:Gallium; D019855:Ethylene Glycol