title: [Expression of a secreted fusion protein consisting of anti-HER2 antibody and reversed caspase-3 and its suppression on growth of SKOV3 cells].
abstract: OBJECTIVE
  To investigate the killing effect on SKOV3 cells by fusion protein HER2-specific antibody-reversed caspase-3 in the secreted form.
  
  
  METHODS
  The reversed human caspase-3 gene was subcloned into pCMV-e23scFv-PEII-PEIII to construct recombinant eukaryotic expression vector pCMV-e23scFv-PEII-revcasp-3 and transfect Jurkat cells. The secreted expression of the in culture supernatant of transfected Jurkat cells was detected by ELISA. The suppression of the fusion protein on growth of SKOV3 cells was examined by co-culture with supernatant of transfected Jurkat cells.
  
  
  RESULTS
  There was the secreted expression of the fusion protein in Jurkat cells. Expressed product had the activity of inducing SKOV3 cells to death.
  
  
  CONCLUSIONS
  The fusion protein expressed in the secreted form can targeted towards SKOV3 cells and kill them.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Zhang
authors.forename: Lin-Tao
authors.initials: LT
authors.lastname: Jia
authors.forename: Wei
authors.initials: W
authors.lastname: Bao
authors.forename: Cui-Juan
authors.initials: CJ
authors.lastname: Yu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Ming
authors.initials: M
authors.lastname: Jin
authors.forename: Cheng-Ji
authors.initials: CJ
authors.lastname: Wang
authors.forename: An-Gang
authors.initials: AG
authors.lastname: Yang
mesh_terms: D000906:Antibodies / Q000235:genetics; D017209:Apoptosis / Q000235:genetics; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014162:Transfection
chemical_list: D000906:Antibodies; D011993:Recombinant Fusion Proteins; D053148:Caspase 3