title: [Screening of TNF-alpha mimotopes from c7c phage display peptide library].
abstract: OBJECTIVE
  To acquire oligo-peptides mimicking TNF-alpha epitopes.
  
  
  METHODS
  The TNF-alpha mimotopes were screened from c7c phage display peptide library by using neutralizing mAb J1D9 against TNF-alpha, and positive clones were identified by sandwich ELISA.
  
  
  RESULTS
  9 out of 20 phage clones were identified as positive clones which can bind to mAb J1D9. The deduced amino acid sequence analysis showed three different sequences: c-RRPAQSG-c, c-NKHNRKI-c and c-RGMSRKI-c.
  
  
  CONCLUSIONS
  All of the three peptides obtained can mimic the epitopes of TNF-alpha and bind to mAb J1D9.
authors.affiliation: Department of Immunology, First Military Medical University, Guangzhou 510515, China.
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Luo
authors.forename: Bei-Yi
authors.initials: BY
authors.lastname: Liu
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Zhu
authors.forename: Ning
authors.initials: N
authors.lastname: Fu
mesh_terms: D000595:Amino Acid Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D019151:Peptide Library; D010455:Peptides / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000939:Epitopes; D019151:Peptide Library; D010455:Peptides; D014409:Tumor Necrosis Factor-alpha