title: Imaging green fluorescent protein fusions in living fission yeast cells.
abstract: The use of green fluorescent protein (GFP) fusions as biosensors for examining protein localization and dynamics has revolutionized cell biology. Here, we describe the methods developed for imaging of GFP-fusions in the fission yeast Schizosaccharomyces pombe using fluorescence microscopy, with a focus on the use of time-lapse imaging to analyze the dynamics of microtubules. We discuss the considerations in fluorescence microscopy, cell preparation, data acquisition, and image analysis appropriate for analysis of living cells.
authors.affiliation: University of Pennsylvania, Department of Cell and Developmental Biology, 421 Curie Blvd.-BRB2/3 Room 1009, Philadelphia, PA 19104, USA.
authors.forename: P T
authors.initials: PT
authors.lastname: Tran
authors.forename: Anne
authors.initials: A
authors.lastname: Paoletti
authors.forename: Fred
authors.initials: F
authors.lastname: Chang
mesh_terms: D003599:Cytoskeleton / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000032:analysis*; D012568:Schizosaccharomyces / Q000737:chemistry* / Q000166:cytology*
chemical_list: D049452:Green Fluorescent Proteins