ELVIRA HSV, a yield reduction assay for rapid herpes simplex virus susceptibility testing.
A colorimetric yield reduction assay, ELVIRA (enzyme-linked virus inhibitor reporter assay) HSV, was developed to determine the antiviral drug susceptibilities of herpes simplex virus (HSV). It uses an HSV-inducible reporter cell line. This simple and rapid assay has an objective readout, low inoculum size, and good reproducibility. The results correlate well with those of the plaque reduction assay.
Stránská
Ruzena
R
Department of Virology, Eijkman-Winkler Center, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands. r.stranska@azu.nl
Schuurman
Rob
R
Scholl
David R
DR
Jollick
Joseph A
JA
Shaw
Carl J
CJ
Loef
Caroline
C
Polman
Merjo
M
van Loon
Anton M
AM
D000212:Acyclovir / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology*; D003124:Colorimetry; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D017245:Foscarnet / Q000494:pharmacology; D017930:Genes, Reporter / Q000235:genetics; D007763:Lac Operon / Q000235:genetics; D018139:Simplexvirus / Q000187:drug effects* / Q000235:genetics; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D017245:Foscarnet; D000212:Acyclovir