[The anti-HBV effect and mechanism of C gene truncated mutant in vitro].
OBJECTIVE
To explore the effect and mechanism on HBV replication in C gene truncated mutant.


METHODS
Protein expression of C gene truncated vector and wild C gene vector were assay by SDS-PAGE Western blot. Constructed C gene truncated expression vector was cotransfected with wild HBV genome; virus load was detected by PCR in the culture medium and the cell. The formation of core particle was assay by Native western blot.


RESULTS
The recombinant vectors can efficiently express. Virus load of the cotransfected group by pcDNA3-deltaC and adwR9 was lower than that of control group in the culture medium and the cell. Protein band of the co-expressed group by pcDNA3-deltaC and pcDNA3-C showed slightly weaker than that of the co-expressed group by pcDNA3 and pcDNA3-C.


CONCLUSIONS
C gene truncated mutant could interfere with the formation of core particle and reduce of HBV replication
Di
Ya-nan
YN
Institute of Hepatology, Beijing Military General Hospital, Beijing 100700, China.
Hu
Da-rong
DR
Fan
Gong-ren
GR
Hu
Xue-ling
XL
Liu
Chao-ying
CY
Liu
Yong
Y
Wu
Yi-pin
YP
D002460:Cell Line; D015316:Genetic Therapy; D006509:Hepatitis B / Q000628:therapy*; D006801:Humans; D009154:Mutation; D014162:Transfection; D014758:Viral Core Proteins / Q000235:genetics*; D014779:Virus Replication
D014758:Viral Core Proteins