Chondrogenic differentiation of human mesenchymal stem cells using a thermosensitive poly(N-isopropylacrylamide) and water-soluble chitosan copolymer.
Poly(N-isopropylacrylamide) (PNIPAAm) is known to be thermally responsive material and has a lower critical solution temperature (LCST, 32 degrees C) at which a macromolecular transition from a hydrophilic to a hydrophobic structure occurs. Chitosan is a useful natural polymeric biomaterial due to its biocompatibility and biodegradable properties. It has good characteristics for cell attachment, proliferation and viability. The aim of this study was to assess the ability to differentiate from mesenchymal stem cells (MSCs) to chondrocytes and mass formation using a newly developed injectable material, a thermosensitive (water-soluble chitosan-g-PNIPAAm) gel, and evaluate cartilage formation in vivo after injecting a cell-thermosensitive gel complex. The MSCs were cultured in the chitosan-PNIPAAm in vitro. Fluorescence-activated cell sort analysis, viability test, collagen type I, II, X formation and the aggrecan levels were examined. These cultured cells can be easily recovered from a copolymer gel by simply lowering the temperature. An animal study was performed to assess cartilage formation in the submucosal layer of the bladder of rabbits. The cartilage formation could be detected. This can be used to treat vesicoureteral reflux or reflux esophagitis by the effective mass effect. This is a simple method (sol-gel technique in LCST), and good cartilage formation occurs in the bladder tissue.
Cho
Jae Ho
JH
Department of Orthopaedic Surgery, Ajou University College of Medicine, San 5, Wonchun-Dong, Paldal-Gu, Suwon 442-749, South Korea.
Kim
Su-Hyang
SH
Park
Ki Dong
KD
Jung
Myung Chul
MC
Yang
Woo Ick
WI
Han
Sang Won
SW
Noh
Ji Yeun
JY
Lee
Jin Woo
JW
D020341:Absorbable Implants; D000180:Acrylic Resins / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D019902:Chondrocytes / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D011108:Polymers / Q000138:chemical synthesis; D012995:Solubility; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods*; D014867:Water / Q000737:chemistry
D000180:Acrylic Resins; D001672:Biocompatible Materials; D011108:Polymers; D014867:Water; C052970:poly-N-isopropylacrylamide; D048271:Chitosan