The phosphoprotein StarD10 is overexpressed in breast cancer and cooperates with ErbB receptors in cellular transformation.
We have identified that StarD10, a member of the START protein family, is overexpressed in both mouse and human breast tumors. StarD10 was initially discovered on the basis of its cross-reactivity with a phosphoserine-specific antibody in mammary tumors from Neu/ErbB2 transgenic mice and subsequently isolated from SKBR3 human breast carcinoma cells using a multistep biochemical purification strategy. We have shown that StarD10 is capable of binding lipids. StarD10 was found to be overexpressed in 35% of primary breast carcinomas and 64% of human breast cancer cell lines, correlating with their ErbB2/Her2 status. Coexpression of StarD10 with ErbB1/epidermal growth factor receptor in murine fibroblasts enhanced anchorage-independent growth in soft agar, providing evidence for functional cooperation between StarD10 and ErbB receptor signaling. Taken together, these data suggest that overexpression of this lipid-binding protein contributes to breast oncogenesis.
Olayioye
Monilola A
MA
The Walter and Eliza Hall Institute of Medical Research and Bone Marrow Research Laboratories, Royal Melbourne Hospital, Parkville, Victoria, Australia.
Hoffmann
Peter
P
Pomorski
Thomas
T
Armes
Jane
J
Simpson
Richard J
RJ
Kemp
Bruce E
BE
Lindeman
Geoffrey J
GJ
Visvader
Jane E
JE
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000378:metabolism* / Q000473:pathology; D003429:Cross Reactions; D066246:ErbB Receptors / Q000096:biosynthesis / Q000378:metabolism*; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; D006801:Humans; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010750:Phosphoproteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000737:chemistry / Q000276:immunology
D066246:ErbB Receptors; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; D010750:Phosphoproteins; D018719:Erb-b2 Receptor Tyrosine Kinases; D014157:Transcription Factors; C467200:Foxo1 protein, mouse; C488090:STARD10 protein, human; C488091:Stard10 protein, mouse