NK cell depletion diminish tumour-specific B cell responses.
Natural killer (NK) cells can exercise immediate cytotoxicity against malignant cells and thus far modulate the development of tumour directed T cell immunity. To investigate the impact of NK cells on the development of tumour directed B cell immunity mice were immunised with IMR5-75 human neuroblastoma cells with or without prior in vivo NK cell depletion. Flow cytometry analyses gave evidence for an impaired IgG response against the cells immunised with. Dissection of Th1 (IgG2a) and Th2 (IgG1) oriented B cell responses revealed Th1 responses as primarily affected, while Th2 oriented B cell responses as measured by flow cytometry and GD2 ganglioside-specific ELISA were enforced. The data reveal an unexpected impact of NK cells on the development of tumour directed B cell responses. Consequently, NK cell function has also to be taken into account when developing B cell-based cancer immunotherapy.
Jensen
Markus
M
Molecular Tumour Biology and Tumour Immunology, University of Cologne, Cologne, Germany. jensen@uni-koeln.de
Tawadros
Samir
S
Sedlacek
Hans-Harald
HH
Schultze
Joachim L
JL
Berthold
Frank
F
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D000917:Antibody Formation / Q000187:drug effects / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D045744:Cell Line, Tumor; D017690:Cell Transplantation; D003602:Cytotoxicity, Immunologic / Q000187:drug effects / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005677:G(M1) Ganglioside / Q000276:immunology; D005732:Gangliosides / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007132:Immunoglobulin Isotypes / Q000097:blood / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D009374:Neoplasms, Experimental / Q000276:immunology* / Q000473:pathology; D014611:Vaccination; D023041:Xenograft Model Antitumor Assays
D000906:Antibodies; D005732:Gangliosides; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D005677:G(M1) Ganglioside; C019403:ganglioside, GD2; C018835:asialo GM1 ganglioside