Sub-genomic replicons of Tick-borne encephalitis virus.
We constructed three sub-genomic replicons of Tick-borne encephalitis virus (TBEV) (Oshima REP, Oshima REP-GFP and Oshima REP-Neo) by deleting genes coding for structural proteins without or with insertion of green fluorescent protein (GFP) or Neo genes, respectively. BHK cells transfected with Oshima REP expressed the viral non-structural antigens in immunofluorescent and western blot analyses. GFP and viral antigens were co-expressed in the transfected cells with Oshima REP-GFP. G418-resistant cells harboring Oshima REP-Neo consistently expressed the antigens without showing any apparent CPE. These replicons constructed in this study will be useful in studies on the replication, assembly and packaging of TBEV, and to develop vaccines and gene-delivering systems.
Hayasaka
D
D
Division of Clinical Investigation, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.
Yoshii
K
K
Ueki
T
T
Iwasaki
T
T
Takashima
I
I
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004669:Encephalitis Viruses, Tick-Borne / Q000235:genetics*; D017353:Gene Deletion; D005818:Genetic Engineering; D016679:Genome, Viral; D049452:Green Fluorescent Proteins; D019868:Kanamycin Kinase / Q000235:genetics; D008164:Luminescent Proteins; D012093:Replicon; D015678:Viral Structural Proteins / Q000235:genetics; D014779:Virus Replication
D008164:Luminescent Proteins; D015678:Viral Structural Proteins; D049452:Green Fluorescent Proteins; D019868:Kanamycin Kinase