Susceptibility of various mouse strains to systemically- or aerosol-initiated tularemia by virulent type A Francisella tularensis before and after immunization with the attenuated live vaccine strain of the pathogen.
The following study determined whether commonly available mouse strains could be used to reveal vaccines capable of protecting against aerosol exposure to virulent type A strains of Francisella tularensis. The attenuated live vaccine strain (LVS) of the pathogen, F. tularensis LVS, was used as the model vaccine for these studies. The results showed that most mouse strains survived intradermal immunization with 10(5)cfu of LVS, except for A/J mice the majority of which died following exposure to 10(4)cfu (colony forming units). Most LVS-immunized mouse strains survived subsequent i.d. challenge with >50 LD(50) of a virulent type A strain of the pathogen. However, all mouse strains tested remained susceptible to lethal infection by a low dose ( approximately 20 cfu) aerosol challenge with virulent F. tularensis even after vaccination with LVS, though immunized C3H/HeN mice appeared to be slightly more resistant than the rest to such an assault.
Shen
Hua
H
Institute for Biological Sciences, National Research Council Canada, Ottawa, Ont, Canada K1A 0R6.
Chen
Wangxue
W
Conlan
J Wayne
JW
D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D004198:Disease Susceptibility; D005604:Francisella tularensis / Q000276:immunology* / Q000472:pathogenicity; D019570:Inhalation Exposure; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000382:microbiology / Q000473:pathology; D016019:Survival Analysis; D014406:Tularemia / Q000276:immunology* / Q000382:microbiology; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology
D001428:Bacterial Vaccines; D014613:Vaccines, Attenuated