[Development and application of an indirect immunofluorescence assay for the detection of serum immunoglobulin G to human herpesvirus 6].
OBJECTIVE
To establish an indirect immunofluorescence assay(IFA) for detection of serum IgG antibody against human herpesvirus 6(HHV-6).


METHODS
Cord blood mononuclear cells infected by HHV-6 strain CN(5) were used to prepare cell antigen smear, so as to establish an IFA and make an epidemical investigation on serum specimens of child-bearing age, women.


RESULTS
The specificity of the IFA for HHV-6 was confirmed by absorption assay(test). The IFA detection showed that in serum specimens from 116 cases of child-bearing age, women, the positive rate of anti-HHV-6 IgG was 72.4% and geometric mean titer(GMT) was 1:61. The positive rate and GMT of serum anti-HHV-6 IgG had no differences between pregnant women and unpregnant women, neither among pregnant women at different pregnant stages.


CONCLUSIONS
A specific IFA has been developed successfully for epidemical investigation of HHV-6 infection rate in child-bearing women.
Liu
Jun-Lian
JL
Department of Dermatology, 306th Hospital of PLA, Beijing 100101, China.
Xu
Zhi-Kai
ZK
Wang
Hong-Ying
HY
Wang
De-Tang
DT
Zhang
Fang-Ling
FL
Yang
Qiao-Xin
QX
Yao
Kun
K
D000914:Antibodies, Viral / Q000097:blood; D019084:Fluorescent Antibody Technique, Indirect; D006566:Herpesviridae Infections; D015654:Herpesvirus 6, Human; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood
D000914:Antibodies, Viral; D007074:Immunoglobulin G