Expression of KiSS-1, a metastasis suppressor gene, in trophoblast giant cells of the rat placenta.
Metastin is encoded by a putative human metastasis suppressor gene KiSS-1, and is the cognate ligand of a G-protein-coupled receptor designated OT7T175. To study the physiological function(s) of metastin, we cloned rat and mouse KiSS-1 cDNAs both encoding 130-amino acid KiSS-1 proteins. Sequence analysis suggested that processing of the rat and mouse KiSS-1 proteins produces 52-amino-acid peptides, each with an amidated carboxyl terminal and with a single possible disulfide bond, corresponding to rat and mouse metastins. The carboxyl-terminal sequence of metastin, known to be essential for functional receptor interaction, was found to be highly conserved among humans and rodents. Real-time PCR analysis indicated that rat KiSS-1 mRNA showed the highest expression level in the cecum and colon. Since KiSS-1 mRNA and metastin are known to be abundant in human placenta, we further studied the localization of KiSS-1 and OT7T175 mRNAs in rat placenta by in situ hybridization. KiSS-1 and OT7T175 mRNAs were specifically detected in trophoblast giant cells at embryonic day 12.5, and the transcripts in the cells gradually decreased during placental maturation. These results suggest that metastin/OT7T175 signaling may participate in implantation of the mammalian embryo, placenta formation, and maintenance of pregnancy.
Terao
Yasuko
Y
Pharmacology Research Laboratories I, Pharmaceutical Research Division, Takeda Chemical Industries, Ltd., 17-85 Jusohonmachi 2-chome, Yodogawa, Osaka 532-8686, Japan.
Kumano
Satoshi
S
Takatsu
Yoshihiro
Y
Hattori
Masahiko
M
Nishimura
Atsushi
A
Ohtaki
Tetsuya
T
Shintani
Yasushi
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000378:metabolism; D004220:Disulfides; D004305:Dose-Response Relationship, Drug; D010064:Embryo Implantation; D004622:Embryo, Mammalian / Q000378:metabolism; D016147:Genes, Tumor Suppressor; D015726:Giant Cells / Q000378:metabolism*; D017403:In Situ Hybridization; D059648:Kisspeptins; D051379:Mice; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D010455:Peptides / Q000737:chemistry; D010920:Placenta / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013997:Time Factors; D014018:Tissue Distribution; D014327:Trophoblasts / Q000378:metabolism
D018076:DNA, Complementary; D004220:Disulfides; C491407:Kiss1 protein, mouse; C491406:Kiss1 protein, rat; D059648:Kisspeptins; D010455:Peptides; D011506:Proteins; D012333:RNA, Messenger