Mutagenic mechanism of the A-T to G-C transition induced by 5-bromouracil: an ab Initio Study.
The tautomerisms of uracil, 5-bromouracil (BrU), G-U, G-BrU, A-U, and A-BrU have been studied theoretically in an effort to investigate the mutagenicity of BrU. The ab initio calculations have been performed using HF and B3LYP methods with various basis sets. The relative stability of all tautomers was established. The intermolecular interactions between U, BrU, U*, BrU* (asterisks denote enol forms), and water have been studied. It shows that the possibility of tautomerism from BrU to BrU* is much more likely than that from U to U*. Further research indicates that BrU* tends to pair with guanine more easily than U*. The proton transfer process has been investigated by potential energy surface (PES) scan and transition state analysis. The results show that the proton transfer between G-U* and G*-U is monodirectional barrier-free proton transfer (BFPT), which terminates the base mismatch induced by U*. On the other hand, the proton transfer between G-BrU* and G*-BrU is bidirectional BFPT, which makes the base mismatch induced by BrU* sustained. On the basis of all of these calculated results, a new mutagenic mechanism for the A-T to G-C transition induced by 5-bromouracil is described in detail for the first time. It might give a new insight into the origin of the mutagenicity of the 5-Br derivative.
Hu
Xingbang
X
Department of Chemistry, Zhejiang University, Hangzhou 310027, People's Republic of China.
Li
Haoran
H
Ding
Jiayi
J
Han
Shijun
S
D001482:Base Composition / Q000187:drug effects*; D020029:Base Pairing / Q000187:drug effects*; D001976:Bromouracil / Q000737:chemistry* / Q000494:pharmacology*; D008956:Models, Chemical; D009153:Mutagens / Q000737:chemistry / Q000494:pharmacology*; D014498:Uracil / Q000737:chemistry; D014867:Water
D009153:Mutagens; D014867:Water; D001976:Bromouracil; D014498:Uracil