Dentonin, a fragment of MEPE, enhanced dental pulp stem cell proliferation.
Matrix extracellular phosphoglycoprotein (MEPE) is a SIBLING protein, found in bone and dental tissues. The purpose of this study was to determine whether a 23-amino-acid peptide derived from MEPE (Dentonin or AC-100) could stimulate dental pulp stem cell (DPSC) proliferation and/or differentiation. DPSCs were isolated from erupted human molars, and the mitogenic potential of Dentonin in DPSCs was measured by BrdU immunoassay and cell-cycle gene SuperArray. Differentiation of DPSCs with Dentonin was characterized by Western blot and by osteogenesis gene SuperArray. Dentonin enhanced DPSC proliferation by down-regulating P16, accompanied by up-regulation of ubiquitin protein ligase E3A and human ubiquitin-related protein SUMO-1. Enhanced cell proliferation required intact RGD and SGDG motifs in the peptide. This study shows that Dentonin can promote DPSC proliferation, with a potential role in pulp repair. Further studies are required to determine the usefulness of this material in vivo.
Liu
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Box 0640, University of California, San Francisco, CA 94143-0640, USA.
Li
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W
Gao
C
C
Kumagai
Y
Y
Blacher
R W
RW
DenBesten
P K
PK
D000595:Amino Acid Sequence; D015153:Blotting, Western; D001973:Bromodeoxyuridine; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000032:analysis; D018844:Cyclin-Dependent Kinases / Q000032:analysis; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D015536:Down-Regulation; D016326:Extracellular Matrix Proteins / Q000494:pharmacology*; D005353:Fibronectins / Q000032:analysis; D006023:Glycoproteins / Q000494:pharmacology*; D006025:Glycosaminoglycans / Q000032:analysis; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D009842:Oligopeptides / Q000032:analysis; D015676:Osteonectin / Q000032:analysis; D010446:Peptide Fragments / Q000494:pharmacology*; D010750:Phosphoproteins / Q000494:pharmacology*; D011971:Receptors, Immunologic / Q000032:analysis; D025842:SUMO-1 Protein / Q000032:analysis; D013234:Stem Cells / Q000187:drug effects*; D044767:Ubiquitin-Protein Ligases / Q000032:analysis; D015854:Up-Regulation
D019941:Cyclin-Dependent Kinase Inhibitor p16; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D006023:Glycoproteins; D006025:Glycosaminoglycans; C487705:MEPE protein (242-264), human; C486579:MEPE protein, human; D009842:Oligopeptides; D015676:Osteonectin; D010446:Peptide Fragments; D010750:Phosphoproteins; D011971:Receptors, Immunologic; D025842:SUMO-1 Protein; C047981:arginyl-glycyl-aspartic acid; D044767:Ubiquitin-Protein Ligases; D018844:Cyclin-Dependent Kinases; D001973:Bromodeoxyuridine