title: Genoprevalence in human tissues of TT-virus genotype 6.
abstract: TT virus (TTV) is a newly discovered human virus of high genotypic diversity. TTV is widely distributed among humans, but the possible genotype-related differences in TTV biology are not well known. The prevalence and amount of TTV-DNA, especially of genotype 6, was determined by nested-PCR in various human tissues, and human parvovirus B19, another ssDNA virus, was used as a reference. TTV DNA was detected simultaneously in bile, peripheral blood mononuclear cells (PBMC) and plasma of 77% subjects, in 38% skin samples, in 38% synovial samples and in all (100%) adenoids, tonsils and liver samples. The relative concentrations of TTV-DNA did not vary significantly among the different samples. Genotype 6 TTV-DNA was detected in bile and plasma of one subject (3%), in skin and serum of one subject (8%) and in one liver (5%). The overall prevalence of TTV genotype 6 was 4% in subjects and 4% in sera. TTV genotype 6 was shown to occur in human tissues with no obvious tissue-type or symptom specificity. Parvovirus B19 DNA was detected overall in 38% subjects, and bile was the only sample type tested that did not persistently harbour B19 DNA.
authors.affiliation: Department of Virology, Haartman Institute, University of Helsinki and Helsinki University Central Hospital, POB 21,Haartmaninkatu 3, Helsinki, Finland. laura.kakkola@helsinki.fi
authors.forename: L
authors.initials: L
authors.lastname: Kakkola
authors.forename: N
authors.initials: N
authors.lastname: Kaipio
authors.forename: K
authors.initials: K
authors.lastname: Hokynar
authors.forename: P
authors.initials: P
authors.lastname: Puolakkainen
authors.forename: P S
authors.initials: PS
authors.lastname: Mattila
authors.forename: A
authors.initials: A
authors.lastname: Kokkola
authors.forename: E K
authors.initials: EK
authors.lastname: Partio
authors.forename: A-M
authors.initials: AM
authors.lastname: Eis-Hübinger
authors.forename: M
authors.initials: M
authors.lastname: Söderlund-Venermo
authors.forename: K
authors.initials: K
authors.lastname: Hedman
mesh_terms: D000328:Adult; D000368:Aged; D001646:Bile / Q000821:virology; D001769:Blood / Q000821:virology; D004266:DNA Virus Infections / Q000097:blood / Q000453:epidemiology / Q000821:virology; D004279:DNA, Viral / Q000032:analysis* / Q000097:blood; D005260:Female; D005387:Finland / Q000453:epidemiology; D005838:Genotype; D006801:Humans; D008099:Liver / Q000821:virology; D008221:Lymphoid Tissue / Q000821:virology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012867:Skin / Q000821:virology; D013582:Synovial Fluid / Q000821:virology; D022783:Torque teno virus / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004279:DNA, Viral