title: [Isolation and structure identification of the chemical constituents from pine needles of Pinus massoniana Lamb].
abstract: OBJECTIVE
  To study the chemical constituents of the water-extracts of the pine needles of Pinus massoniana Lamb..
  
  
  METHODS
  Pine needles were collected in Xixia country and extracted with boiling water for two times and the water-extracts were concentrated. The crude extract of pine needles was fractionated into four fractions by Et2O, EtOAc and n-BuOH. Various column chromatography with D-101 macroreticular resin, Toyopearl HW-40 and silica gel were employed for the isolation and purification of compounds from the n-BuOH fraction of pine needles. The structures of the compounds were identified by physiochemical properties and spectral analysis (UV, IR, MS, 1HNMR, 13CNMR, DEPT, HMQC, HMBC, etc.).
  
  
  RESULTS
  Four compounds were isolated from the n-BuOH fraction of water-extracts, and their structures were identified as 3-methoxyl-9'-O-alpha-L-rhamnopyranosyl-4':7,5':8-diepoxyneoligan-4,9-diol (massonianoside E, I), 4,4',8,8',9-pentahydroxyl-3,3'-dimethoxyl-7,9'-monoepoxylignan (II), umbelliferon (III), 4-(4'-hydroxyl-3'-methoxylbenzyl)-2-butanone (IV).
  
  
  CONCLUSIONS
  Compound I is a new compound, and compounds II, III and IV were isolated from this plant for the first time.
authors.affiliation: Henan College of Traditional Chinese Medicine, Zhengzhou 450008, China. fwsh@371.net
authors.forename: Wei-sheng
authors.initials: WS
authors.lastname: Feng
authors.forename: Yan-zhi
authors.initials: YZ
authors.lastname: Wang
authors.forename: Xiao-ke
authors.initials: XK
authors.lastname: Zheng
authors.forename: Yue-feng
authors.initials: YF
authors.lastname: Bi
mesh_terms: D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification*; D008968:Molecular Conformation; D015394:Molecular Structure; D028223:Pinus / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D014468:Umbelliferones / Q000737:chemistry / Q000302:isolation & purification
chemical_list: C488566:3-methoxyl-9'-O-alpha-L-rhamnopyranosyl-4',7-diepoxyneoligan-4,9-diol; D017705:Lignans; D014468:Umbelliferones