title: [Construction and identification of recombinant retrovirus vector containg hTrx gene].
abstract: OBJECTIVE
  To construct a recombinant retrovirus vector carrying hTrx gene.
  
  
  METHODS
  hTrx gene was cloned directionally into a retroviral vector pLXSN by DNA recombinant technique and the recombinant vector was identified EcoRI and BamHI digestion analysis. The recombinant was transfected into packaging cells PA317 via CaPO(4)-based transfection method, and viral titer in culture supernatant of transfected cells was detected.
  
  
  RESULTS
  The recombinant pLTrxSN had been constracted. EcoRI and BamHI digestion analysis displayed two positive bands of 0.3 kb and 5.9 kb respectively. The viral titer in supernatant of transfected cells was 5.5x10(9) cfu/L.
  
  
  CONCLUSIONS
  pLhTrxSN has been constructed successfully, which will be useful for Trx gene therapy and experiment study.
authors.affiliation: Neuroscience Center of Xi'an Jiaotong University Xi'an 710061, China.
authors.forename: Min-Jie
authors.initials: MJ
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Zhao
authors.forename: Quan-Ying
authors.initials: QY
authors.lastname: Wang
authors.forename: Guang-Xiao
authors.initials: GX
authors.lastname: Yang
mesh_terms: D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D012190:Retroviridae / Q000235:genetics; D014162:Transfection