title: [The construction and expression of a hepatocellular carcinoma-specific superantigen SEA (D227A) expression vector].
abstract: OBJECTIVE
  To construct the superantigen SEA (D227A) expression vector modulated by cis-acting element of AFP and express it specifically on AFP(+) hepatocellular carcinoma (HCC) cells.
  
  
  METHODS
  The enhancer and promoter of AFP gene, SEA (D227A) cDNA and linker-CD80tm were amplified by PCR. The gene segments mentioned above were cloned into the multiple cloning sites of retrovirus vector pLXSN to construct an attenuated superantigen expression vector pLXSN-SEA (D227A)-linker-CD80tm modulated by cis-acting element of AFP gene. AFP(+) or AFP(-) HCCs were transfected by the superantigen expression vector through lipofectamine mediation. SEA expression was detected by RT-PCR and indirect immunofluorescence staining.
  
  
  RESULTS
  Promoter, enhancer, SEA (D227A) and linker-CD80tm had been successfully cloned into the multiple cloning site of retrovirus vector pLXSN RT-PCR and indirect immunofluorescence assay were used to prove that SEA (D227A) could specifically express in the AFP(+) HCCs.
  
  
  CONCLUSIONS
  The successful construction and expression of hepatocellular carcinoma-specific superantigen SEA (D227A) gene expression vector lay the foundation for enhancing immunotherapy of hepatocellular carcinoma.
authors.affiliation: Department of General Surgery, First Hospital of Xi'an Jiaotong University, Xi'an 710061 China.
authors.forename: Shao-Ying
authors.initials: SY
authors.lastname: Lu
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Sui
authors.forename: Zeng-Shan
authors.initials: ZS
authors.lastname: Li
authors.forename: Cheng-En
authors.initials: CE
authors.lastname: Pan
authors.forename: Jing
authors.initials: J
authors.lastname: Ye
authors.forename: Xiu-Min
authors.initials: XM
authors.lastname: Zhang
authors.forename: Wen-Yong
authors.initials: WY
authors.lastname: Wang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics; D018089:Superantigens / Q000276:immunology; D014162:Transfection; D000509:alpha-Fetoproteins / Q000235:genetics
chemical_list: D018089:Superantigens; D000509:alpha-Fetoproteins