[Mutations of the androgen receptor gene in patients with clinically localized adenocarcinoma of the prostate].
The aim of this study was to detect mutations in the human androgen receptor gene in radical prostatectomy specimens.


METHODS
The genomic sequence was realized in 67 radical prostatectomy specimens. The mean age was 64 years old. The PSA median was 15 ng/ml. TNM 1997: 34.3% were T1 and 65.7% T2. Genomic sequence: 1. Radical prostatectomy specimens desparaffitation. 2. Extraction of the DNA 3. DNA amplification. 4. Automatic genome sequence. 5. Comparison with Gene-Bank.


RESULTS
16.7% of the specimens were mutated. The most frequent mutation was the punctual mutation. The exon most frequent mutated was exon 1.
Sánchez Zalabardo
D
D
Departamento de Urología, Clínica Universitaria, Universidad de Navarra, Pamplona.
Rosell Costa
D
D
Honorato Cia
B
B
Rioja Zuazu
J
J
Regojo Balboa
J M
JM
Fernández Montero
J M
JM
López Ferrandis
J
J
Robles García
J E
JE
Zudaire Bergera
J J
JJ
García Foncillas
J
J
Berián Polo
J M
JM
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000368:Aged; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D011944:Receptors, Androgen / Q000235:genetics*
D011944:Receptors, Androgen