Characterization of the glycosyltransferase activity of desVII: analysis of and implications for the biosynthesis of macrolide antibiotics.
In vitro catalytic activity of DesVII, the glycosyltransferase involved in the biosynthesis of methymycin, neomethymycin, narbomycin, and pikromycin in Streptomyces venezuelae, is described. This is the first report of demonstrated in vitro activity of a glycosyltransferase involved in the biosynthesis of macrolide antibiotics. DesVII is unique among glycosyltransferases in that it requires an additional protein component, DesVIII, as well as basic pH for its full activity.
Borisova
Svetlana A
SA
Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712, USA.
Zhao
Lishan
L
Melançon III
Charles E
CE
Kao
Chai-Lin
CL
Liu
Hung-Wen
HW
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D006031:Glycosylation; D016695:Glycosyltransferases / Q000378:metabolism*; D018942:Macrolides / Q000378:metabolism*; D015394:Molecular Structure; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics
D000900:Anti-Bacterial Agents; D018942:Macrolides; C008517:methymycin; D016695:Glycosyltransferases; C003145:picromycin