title: Uptake and remodeling of exogenous phosphatidylethanolamine in E. coli.
abstract: The fate of exogenous short-chain analogues of phosphatidylethanolamine and phosphatidylserine was studied in a deep-rough derivative of E. coli mutant strain AD93 that cannot synthesize phosphatidylethanolamine de novo. Using mass spectrometry, it was shown that dicaproyl(di 6:0)-phosphatidylethanolamine is extensively remodeled, eventually adopting the phosphatidylethanolamine species profile of the parental wild-type strain of AD93. Dicaproyl-phosphatidylserine was decarboxylated to form phosphatidylethanolamine, and yielded a species profile, which strongly resembled that of the introduced phosphatidylethanolamine. This demonstrates transport of phosphatidylserine to the cytosolic leaflet of the inner membrane. The changes of the species profile of phosphatidylethanolamine indicate that the short-chain phospholipids are most likely remodeled via two consecutive acyl chain substitutions, and at least part of this remodeling involves transport to the inner membrane.
authors.affiliation: Department of Biochemistry of Membranes, Institute of Biomembranes, Ctr. Biomem. and Lipid Enz. (CBLE), Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors.forename: Matthijs A
authors.initials: MA
authors.lastname: Kol
authors.forename: Diederik W D
authors.initials: DW
authors.lastname: Kuster
authors.forename: Henry A
authors.initials: HA
authors.lastname: Boumann
authors.forename: Hans
authors.initials: H
authors.lastname: de Cock
authors.forename: Albert J R
authors.initials: AJ
authors.lastname: Heck
authors.forename: Ben
authors.initials: B
authors.lastname: de Kruijff
authors.forename: Anton I P M
authors.initials: AI
authors.lastname: de Kroon
mesh_terms: D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D013058:Mass Spectrometry; D010714:Phosphatidylethanolamines / Q000378:metabolism*
chemical_list: D010714:Phosphatidylethanolamines; C483858:phosphatidylethanolamine