title: [Study on detecting the sensitivity of tumor cells to chemotherapeutic agents by immunohistochemical staining with anti-ssDNA mAb].
abstract: OBJECTIVE
  To explore the possibility of application of self-made anti-ssDNA mAb to detecting the apoptosis of tumor cells induced by chemotherapeutic drugs and evaluate the sensitivity of tumor cells to chemotherapeutic drugs from a apoptotic rate.
  
  
  METHODS
  Apoptosis of both K562 and Hep-2 cells were induced by maximum plasma concentrations of 9 different chemotherapeutic drugs. The apoptosis of tumor cells was detected by immunohistochemical ( IHC) staining with anti-ssDNA mAb and their apoptotic rate were calculated.
  
  
  RESULTS
  The apoptotic cells could be differentiated from non-apoptotic cells by the HIC staining with anti-ssDNAmAb. The apoptosis of tumor cells were induced by chemotherapeutic drugs, but apoptotic extent of different tumor cells, even the same one tumor cell, was distinct when they exposed to all kinds of anti-tumor drugs.
  
  
  CONCLUSIONS
  The combination of anti-ssDNA mAb with IHC staining can be used to detect the sensitivity of tumor cells to chemotherapeutic drugs.
authors.affiliation: Institute of Immunology, Weifang Medical College, Weifang 261042, China.
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Wu
authors.forename: Zhi-wei
authors.initials: ZW
authors.lastname: Xu
authors.forename: Guo-qing
authors.initials: GQ
authors.lastname: Wu
authors.forename: Nai-fa
authors.initials: NF
authors.lastname: Miao
authors.forename: Yong-tang
authors.initials: YT
authors.lastname: Feng
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D004277:DNA, Single-Stranded / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D020014:K562 Cells / Q000187:drug effects; D051379:Mice; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology; D013194:Staining and Labeling
chemical_list: D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D004277:DNA, Single-Stranded