[Immunohistochemical study of in situ CD86 expression in rejected rat corneal graft after penetrating keratoplasty].
OBJECTIVE
To investigate the expression of CD86 co-stimulatory molecules in rejected rat corneal graft in situ after penetrating keratoplasty.


METHODS
Rat models of orthotopical corneal transplantation were established, and after the occurrence of graft rejection, immunohistochemical staining was performed on the corneal whole-mounts and the spleen tissue of the recipient rats.


RESULTS
CD86(+) cells were detected in the epithelium of the corneal graft during allograft rejection but not in normal cornea.


CONCLUSIONS
In situ expression of costimulatory molecules in the corneal allograft may be related to the acute immune rejection after penetrating keratoplasty.
Bai
Lang
L
Department of Ophthalmology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
Lu
Xiao-he
XH
Zhou
Jin
J
Dang
Sen-tao
ST
Zhang
Jin-hua
JH
D000818:Animals; D015703:Antigens, CD / Q000032:analysis*; D018122:B7-1 Antigen / Q000032:analysis; D051940:B7-2 Antigen; D005260:Female; D006084:Graft Rejection / Q000276:immunology*; D007150:Immunohistochemistry; D015948:Keratoplasty, Penetrating / Q000276:immunology*; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D014184:Transplantation, Homologous
D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497966:Cd86 protein, rat; D008562:Membrane Glycoproteins