title: Receptor tyrosine phosphatase zeta/beta in astrocyte progenitors in the developing chick spinal cord.
abstract: We cloned a cDNA encoding the receptor-type protein tyrosine phosphatase zeta/beta (RPTPZ/beta) from embryonic chick spinal cord. RPTPZ/beta was expressed throughout the ventricular zone (VZ) of the developing spinal cord and in scattered cells outside the VZ. Platelet-derived growth factor receptor alpha (PDGFRa)-positive oligodendrocyte progenitors co-expressed RPTPZ/beta within the VZ but down-regulated RPTPZ/beta after leaving the VZ. Most RPTPZ/beta-positive cells outside the VZ co-expressed glutamine synthetase and fibroblast growth factor receptor-3, indicating that they are astrocyte progenitors. Northern blot analysis revealed a single approximately 9 kbp RPTPZ/beta transcript expressed in the embryonic chick spinal cord, indicating that the shorter alternative-splice products of RPTPZ/beta found in rodent spinal cord and brain--including the abundant extracellular proteoglycan known as phosphacan--are not present in the embryonic chick spinal cord.
authors.affiliation: Wolfson Institute for Biomedical Research, Department of Biology, University College London, Gower Street, London WC1E 6BT, UK.
authors.forename: Anna
authors.initials: A
authors.lastname: Ivanova
authors.forename: Mahima
authors.initials: M
authors.lastname: Agochiya
authors.forename: Marc
authors.initials: M
authors.lastname: Amoyel
authors.forename: William D
authors.initials: WD
authors.lastname: Richardson
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D015152:Blotting, Northern; D002642:Chick Embryo; D015870:Gene Expression / Q000502:physiology; D020869:Gene Expression Profiling; D015723:Gene Library; D017403:In Situ Hybridization; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D013116:Spinal Cord / Q000196:embryology* / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D017027:Protein Tyrosine Phosphatases