Serial bull cloning by somatic cell nuclear transfer.
Although the list of species successfully cloned continues to grow, serial cloning has not been reported in species other than the mouse. Here we describe two live births of second-generation clones of a bull. Clones of the first and second generations appear healthy and have normal telomere lengths. Our attempts to produce the third generation of clones were unsuccessful.
Kubota
Chikara
C
Laboratory of Cell Genetics and Embryo Transfer, Kagoshima Prefectural Cattle Breeding Development Institute, Japan.
Tian
X Cindy
XC
Yang
Xiangzhong
X
D000034:Abortion, Veterinary; D000818:Animals; D001757:Blastomeres / Q000166:cytology; D002417:Cattle; D002452:Cell Count; D002970:Cleavage Stage, Ovum / Q000166:cytology; D019976:Cloning, Organism / Q000379:methods*; D004624:Embryo Transfer / Q000662:veterinary; D005260:Female; D005298:Fertility / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism; D008297:Male; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D019098:Telomerase / Q000235:genetics / Q000378:metabolism; D016615:Telomere / Q000235:genetics / Q000378:metabolism
D019098:Telomerase