Isolation and characterization of subgenomic DNAs encapsidated in "single" T = 1 isometric particles of Maize streak virus.
"Single" T = 1 isometric particles of Maize streak virus (MSV) have been isolated from infected maize leaves. Biochemical and genetic characterizations show that these particles contain subgenomic (sg) MSV DNA encapsidated by the MSV coat protein. The largest sg DNA is 1.56 kb, slightly larger than half genome size, although sg DNAs as small as 0.2 kb were also cloned. The sg DNAs are not infectious, and they do not appear to play a role in the pathogenicity of MSV. This is the first report of sg DNAs for MSV and, to our knowledge, the first time that encapsidated sg DNAs have been characterized at the sequence level for any geminivirus. These data will assist in our investigations into the role of genomic DNA in the formation of the unique geminate capsid architecture of the Geminiviridae.
Casado
Carolina G
CG
Department of Biochemistry and Molecular Biology, College of Medicine, McKnight Brain Institute, University of Florida, Gainesville, FL 32610-0245, USA.
Javier Ortiz
G
G
Padron
Eric
E
Bean
Samantha J
SJ
McKenna
Robert
R
Agbandje-McKenna
Mavis
M
Boulton
Margaret I
MI
D036022:Capsid Proteins; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D016679:Genome, Viral; D026762:Maize streak virus / Q000235:genetics*; D008854:Microscopy, Electron; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D014764:Viral Proteins / Q000378:metabolism; D014771:Virion / Q000737:chemistry* / Q000378:metabolism; D003313:Zea mays / Q000821:virology
D036022:Capsid Proteins; D004279:DNA, Viral; D014764:Viral Proteins