Transfection efficiency and cytotoxicity of cationic liposomes in salmonid cell lines of hepatocyte and macrophage origin.
The transfection efficiency of liposome-based DNA formulations was studied in different salmonid cell lines of hepatocyte and macrophage origin. Parallel assessment of cell viability was carried out to define the balance between transfection efficiency and toxicity. For all cell lines, transfection efficiency varied with the lipoplex charge ratio and the amount of DNA added to the liposomes. The hepatocyte-derived cell line was most readily transfected while lower transfection efficiency was observed for the macrophage cell lines. The cationic liposomes showed a dose-dependent toxicity and were found to be most toxic for cells of macrophage origin. This was in line with the observation that higher amounts of lipids were associated with the cells of macrophage origin than the hepatocytes. Complexing DNA with the liposomes reduced the toxicity for all three cell lines, most markedly, however, for macrophage cell lines. The differences in the transfection and toxicity patterns between the cell lines are probably caused by differences in membrane composition as well as differences in phagocytic activity and processing of the liposomes/lipoplexes.
Romøren
Kristine
K
Department of Pharmaceutics, School of Pharmacy, University of Oslo, Norway.
Thu
Beate J
BJ
Bols
Niels C
NC
Evensen
Øystein
Ø
D000818:Animals; D002412:Cations; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000235:genetics; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry / Q000633:toxicity; D005453:Fluorescence; D022781:Hepatocytes / Q000166:cytology / Q000502:physiology*; D008081:Liposomes / Q000737:chemistry* / Q000633:toxicity*; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D008264:Macrophages / Q000166:cytology / Q000502:physiology*; D010714:Phosphatidylethanolamines / Q000737:chemistry / Q000633:toxicity; D000644:Quaternary Ammonium Compounds / Q000737:chemistry / Q000633:toxicity; D012487:Salmonidae; D055672:Static Electricity; D014162:Transfection / Q000379:methods*
C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D002412:Cations; D005229:Fatty Acids, Monounsaturated; D008081:Liposomes; D010714:Phosphatidylethanolamines; D000644:Quaternary Ammonium Compounds; D004247:DNA; D008156:Luciferases; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane