Microcalorimetric investigation on the growth model and the protein yield of Bacillus thuringiensis.
A novel microcalorimetric technique based on the bacterial heat output was applied to evaluate the special growth model, the protein expression and the generation time of Bacillus thuringiensis for the first time. The thermogenic curves of the aerobic metabolism of B. thuringiensis strains YBT-833, YBT-1520 and YBT-833-2-1 were determined by using an LKB-2277 BioActivity Monitor. The analysis of the thermogenic curves indicated both the mutant strain and the wild-type strains followed the same linear growth model during sporulation. The metabolism heat output revealed heat output was correlated to the yield of the insecticidal crystal proteins (ICPs) very well, the more protein product, and the less heat output. Based on the data acquired, we proposed that this method could be a useful tool in monitoring the fermentation of B. thuringiensis.
Xiaoyan
Lin
L
Key Laboratory of Agricultural Microbiology of Chinese Agriculture Ministry, Life Science and Technology College, Huazhong Agriculture University, Wuhan 430070, Hubei, PR China. linxiaoyan@hotmail.com
Yi
Liu
L
Peng
Liu
L
Songsheng
Qu
Q
Ziniu
Yu
Y
D001413:Bacillus thuringiensis / Q000254:growth & development* / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000096:biosynthesis*; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002151:Calorimetry / Q000379:methods*; D049109:Cell Proliferation; D003198:Computer Simulation; D004731:Endotoxins / Q000096:biosynthesis*; D004734:Energy Metabolism / Q000502:physiology*; D006460:Hemolysin Proteins; D008954:Models, Biological
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis