Lack of association between Alzheimer's disease and the promoter region polymorphisms of the nicastrin gene.
The biological analysis of nicastrin (NCSTN) shows its crucial role in gamma-cleavage of the amyloid precursor protein. Inhibition of NCSTN demonstrated altered gamma-cleavage activity, suggesting its potential implication in Alzheimer's disease (AD). We sequenced the NCSTN gene promoter region and found two promoter single nucleotide polymorphisms (SNPs) at putative transcription binding sites, -796T/G and -1216C/A. The association study using the promoter SNPs showed no significant genetic effect upon the development of AD. Haplotype analysis with the promoter SNPs and coding SNPs demonstrated no significant difference between familial AD cases and controls. Moreover, the genotype of each promoter SNP did not have an association with age-at-onset in AD. Our investigation suggests that the two promoter SNPs are unrelated to the development of AD, however, further investigation at the promoter region of NCSTN may be necessary to address its potential implication of gene expression in AD.
Orlacchio
Antonio
A
Laboratorio di Neurogenetica, IRCCS Santa Lucia, Rome, Italy. a.orlacchio@hsantalucia.it
Kawarai
Toshitaka
T
Polidoro
Mario
M
Paterson
Andrew D
AD
Rogaeva
Ekaterina
E
Orlacchio
Aldo
A
St George-Hyslop
Peter H
PH
Bernardi
Giorgio
G
D000328:Adult; D017668:Age of Onset; D000368:Aged; D000409:Alanine / Q000235:genetics; D000483:Alleles; D000544:Alzheimer Disease / Q000235:genetics*; D053829:Amyloid Precursor Protein Secretases; D001057:Apolipoproteins E / Q000235:genetics; D003545:Cysteine / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005998:Glycine / Q000235:genetics; D006239:Haplotypes; D006801:Humans; D016015:Logistic Models; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013912:Threonine / Q000235:genetics
D001057:Apolipoproteins E; D008562:Membrane Glycoproteins; C413604:nicastrin protein; D013912:Threonine; D053829:Amyloid Precursor Protein Secretases; D003545:Cysteine; D000409:Alanine; D005998:Glycine