title: In vitro activity of amphotericin B, fluconazole and voriconazole against 162 Cryptococcus neoformans isolates from Africa and Cambodia.
abstract: In order to determine the potential role that various antifungal agents might have in the management of cryptococcosis in tropical areas, the in vitro susceptibility of Cryptococcus neoformans isolates from Africa ( n=52) and Cambodia ( n=110) to three antifungal agents (amphotericin B, fluconazole and voriconazole) were compared using the E-test method. The results of this study (i) confirm the value of the E-test for testing the in vitro susceptibility of C. neoformans towards voriconazole; (ii) provide the first evidence demonstrating good activity of amphotericin B, fluconazole and voriconazole against Cambodian isolates; and (iii) show there are differences in susceptibility between African and Asian C. neoformans isolates, with Cambodian isolates appearing less susceptible to the agents tested but with amphotericin B maintaining good activity.
authors.affiliation: Service de Parasitologie-Mycologie-Médecine Tropicale, Centre Hospitalier et Universitaire, 37000 Tours, France. jac-bri.chandenier@wanadoo.fr
authors.forename: J
authors.initials: J
authors.lastname: Chandenier
authors.forename: K D
authors.initials: KD
authors.lastname: Adou-Bryn
authors.forename: C
authors.initials: C
authors.lastname: Douchet
authors.forename: B
authors.initials: B
authors.lastname: Sar
authors.forename: M
authors.initials: M
authors.lastname: Kombila
authors.forename: D
authors.initials: D
authors.lastname: Swinne
authors.forename: M
authors.initials: M
authors.lastname: Thérizol-Ferly
authors.forename: Y
authors.initials: Y
authors.lastname: Buisson
authors.forename: D
authors.initials: D
authors.lastname: Richard-Lenoble
mesh_terms: D000349:Africa / Q000453:epidemiology; D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology; D002160:Cambodia / Q000453:epidemiology; D003453:Cryptococcosis / Q000175:diagnosis / Q000453:epidemiology; D003455:Cryptococcus neoformans / Q000187:drug effects* / Q000302:isolation & purification; D003906:Developing Countries; D025141:Drug Resistance, Fungal; D005260:Female; D015725:Fluconazole / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D011743:Pyrimidines / Q000494:pharmacology*; D018570:Risk Assessment; D012494:Sampling Studies; D014230:Triazoles / Q000494:pharmacology*; D065819:Voriconazole
chemical_list: D000935:Antifungal Agents; D011743:Pyrimidines; D014230:Triazoles; D000666:Amphotericin B; D015725:Fluconazole; D065819:Voriconazole