[Microbial receptor competitive binding assay of basic macrolide antibiotics].
OBJECTIVE
To establish a biological method for determining the macrolide content in various matrices. METHODS Human serum, urine and tissue homogenate samples were diluted or extracted with MSU buffer, and the specimens of grain and premixed feed extracted with methanol-H(3)PO(4) buffer, before microbial receptor competitive binding assay was carried out on these various specimens, with the elimination of interference from methanol with the M8 buffer.


RESULTS
The method was sensitive, class-specific and precise, and the recommended screening concentrations for specimens of the serum, urine, tissue and grains were 200, 200, 100 and 1 200 ng/g (ng/ml), respectively, with a relative standard deviation less than 8%.


CONCLUSIONS
Microbial receptor competitive binding assay is accurate and rapid for efficient qualitative and quantitative assay of the total macrolide content in various matrices.
Qin
Yan
Y
Food Inspection Center, Guangzhou Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510623, China. maomaodan@163.net
Zhang
Mei-jin
MJ
Lin
Feng
F
D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D001667:Binding, Competitive; D018942:Macrolides / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D018942:Macrolides