Decreased expression of the pancreatic secretory trypsin inhibitor II gene during aging of the rat liver.
The genetic constitution and differential gene expression of an organism play important roles in controlling the species-specific rate of aging and the maximum life span potential. We utilized a differential-display polymerase chain reaction technique to identify the age-dependent expression of genes in the rat liver. We demonstrate in this report, for the first time, that expression of the pancreatic secretory trypsin inhibitor II (PSTI-II) gene declines drastically during aging. We confirmed this decrease by Northern blot analysis. Low PSTI-II levels in aged animals might result in a lack of protection from prematurely activated trypsin-like proteases, which would thus enhance inflammation.
Panda
Harekrushna
H
Institute of Life Sciences, Bhubaneswar, India.
Zaidi
Ghazala
G
Supakar
Prakash C
PC
D000375:Aging / Q000235:genetics*; D000818:Animals; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics* / Q000502:physiology; D008099:Liver / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011916:Rats, Inbred F344
D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; C497897:pancreatic secretory trypsin inhibitor II, rat