High cell density fed-batch cultivation of Escherichia coli using exponential feeding combined with pH-stat.
A new feeding strategy in fed-batch culture, exponential feeding combined with pH-stat is suggested to avoid the accumulation of substrate in culture broth. Exponential feeding was stopped whenever a predetermined amount of limiting substrate was supplied and then pH change was observed. When pH rose above an upper limit due to the depletion of substrate, feeding was restarted. With this feeding strategy, recombinant Escherichia coli could be grown to 101 g/l by controlling the specific growth rate at 0.1 h(-1).
Kim
Beom Soo
BS
Department of Chemical Engineering, Chungbuk National University, Cheongju, Korea. bskim@chungbuk.ac.kr
Lee
Seung Chul
SC
Lee
Sang Yup
SY
Chang
Yong Keun
YK
Chang
Ho Nam
HN
D019342:Acetic Acid / Q000378:metabolism*; D000641:Ammonia / Q000378:metabolism*; D001431:Bacteriological Techniques; D003470:Culture Media; D004926:Escherichia coli / Q000254:growth & development* / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods*
D003470:Culture Media; D000641:Ammonia; D005947:Glucose; D019342:Acetic Acid