title: [A comparative analysis of genomes of virulent and avirulent strains of Vibrio cholerae O139].
abstract: A comparative analysis of the genome of V. cholerae O139 strains isolated in Russia's territory from patients with cholera and from the environment showed essential differences in their structures. The genome of clinical strains possessed all tested genes associated with virulence (ctxAB, zot, ace, rstC, rtxA, hap, toxR and toxT) and the at-tRS site for the CTXp phage DNA integration. As for the O139 V. cholerae chromosome strains isolated from water, 70% of the studied genes (ctxAB, zot, ace, rstC, tcpA, and toxT) and the attRS sequence were not detected in them. A lack of the key virulence genes in O139-serogroup "water" vibrios, including genes of toxin-coregulated adhesion pili. (that are receptors for the CTXp phage), and of the attachment site of the above phage are indicative of that the O139 V. cholerae strains isolated from open water sources located in different Russia's regions are epidemically negligible.
authors.affiliation: Mikrob Russian Research Anti-Plague Institute, Saratov.
authors.forename: G A
authors.initials: GA
authors.lastname: Eroshenko
authors.forename: A V
authors.initials: AV
authors.lastname: Osin
authors.forename: E Iu
authors.initials: EIu
authors.lastname: Shchelkanova
authors.forename: N I
authors.initials: NI
authors.lastname: Smirnova
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D002771:Cholera / Q000382:microbiology*; D002772:Cholera Toxin / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D004731:Endotoxins; D016680:Genome, Bacterial; D006801:Humans; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000235:genetics; D011506:Proteins / Q000235:genetics; D012426:Russia; D012697:Serine Endopeptidases / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D041242:Vibrio cholerae O139 / Q000235:genetics* / Q000472:pathogenicity; D037521:Virulence Factors / Q000235:genetics; D014871:Water Microbiology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004268:DNA-Binding Proteins; D004731:Endotoxins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011506:Proteins; C434464:RtxA protein, Vibrio cholerae; D014157:Transcription Factors; D037521:Virulence Factors; C499324:toxR protein, Vibrio cholerae; C066719:toxR protein, bacteria; C077728:zonula occludens toxin, Vibrio cholerae; C075626:tcpN protein, Vibrio cholerae; D002772:Cholera Toxin; D012697:Serine Endopeptidases; D000251:Adenosine Triphosphatases