title: Technique review: how to use RNA interference.
abstract: RNA interference (RNAi) has been rapidly adopted as a general method for inhibiting gene expression in most laboratory organisms. This paper discusses how libraries of RNAi reagents are being used to perform genome-wide reverse genetic screens in both model organisms and mammalian cells. Guidelines for designing effective small interfering RNAs and appropriate controls for mammalian RNAi experiments will also be discussed.
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK. bll@sanger.ac.uk
authors.forename: Ben
authors.initials: B
authors.lastname: Lehner
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Fraser
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Sanderson
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015723:Gene Library; D005821:Genetic Techniques; D006801:Humans; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D011995:Recombination, Genetic
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering