FISH mapping for helminth genome.
Basic techniques for fluorescence in situ hybridization (FISH) mapping that have been used in genome projects on schistosomes and filariae are introduced. The chapter shows techniques specific for bacterial artificial chromosome (BAC) and yeast artificial chromosome (YAC) clones and includes experiences of chromosome preparation, DNA labeling, hybridization, microscopy, and localization of BAC clones.
Hirai
Hirohisa
H
Center for Human Evolution Modeling Research, Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Inuyama, Aichi, Japan.
Hirai
Yuriko
Y
D000818:Animals; D016678:Genome; D006376:Helminths / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*