title: Echinococcus granulosus coproantigens: chromatographic fractionation and characterization.
abstract: Dogs infected with adult tapeworms of Echinococcus granulosus release antigens (coproantigens) in faeces which can be detected by a capture ELISA. Supernatants prepared from E. granulosus-infected dog faecal samples were fractionated by size-exclusion fast protein liquid chromatography (FPLC) on a Superose-6 column. Coproantigen ELISA and Western blotting were used to demonstrate the immunoreactivity of eluted fractions. Two main FPLC peaks of antigenic activity were detected and designated as fraction F1 and fraction F2 with approximate relative molecular weights > 670 kDa, and in the range of 146 to 440 kDa respectively. These two antigenic fractions (F1 and F2) fractionated from infected dog faeces were heat stable and largely protease-insensitive, but were highly sensitive to sodium periodate treatment, which strongly suggested the involvement of carbohydrates. Capture IgG antibodies against E. granulosus proglottis somatic extracts, detected a molecule with an approximate molecular weight of 155 kDa in fraction F2 after immunoblotting. The 155 kDa antigen could be completely ablated by sodium periodate treatment, but not after protease or lipase treatment. A surface tegument preparation of adult E. granulosus tapeworms contained large amounts of antigen that corresponded in size range and antigenicity to that observed in the FPLC fraction F2. There was also a peak of antigenic activity at > 670 kDa corresponding to fraction F1 from a culture derived excretory-secretory (E-S) adult tapeworm preparation. The involvement of carbohydrate moieties in coproantigen activity present in the FPLC fractions F1 and F2 from faecal supernatants of E. granulosus-infected dogs was confirmed by lectin-binding assays and exoglycosidase treatment, which showed that alpha-D-mannose and/or alpha-D-glucose, beta-galactose and N-acetyl-beta-glucosamine residues were the most important carbohydrate components in putative coproantigens present in both fractions. N-acetyl-beta-glucosamine and sialic acid residues were also contained in coproantigen molecules present in fraction F2. These results suggested that coproantigens detected in faeces of E. granulosus-infected dogs are large molecular weight molecules that may be derived from the carbohydrate-rich surface glycocalyx of adult worms, and are shed, released or secreted during the life-span of the tapeworm.
authors.affiliation: Cestode Zoonoses Research Group, Biosciences Research Institute, School of Environment and Life Sciences, University of Salford, Salford M5 4WT, UK.
authors.forename: F A
authors.initials: FA
authors.lastname: Elayoubi
authors.forename: P S
authors.initials: PS
authors.lastname: Craig
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000302:isolation & purification*; D050260:Carbohydrate Metabolism; D002850:Chromatography, Gel; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs; D004443:Echinococcosis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D004446:Echinococcus / Q000254:growth & development* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology*; D006026:Glycoside Hydrolases / Q000378:metabolism; D006358:Hot Temperature; D037102:Lectins / Q000378:metabolism; D008970:Molecular Weight; D010504:Periodic Acid / Q000378:metabolism
chemical_list: D000947:Antigens, Helminth; D037102:Lectins; D010504:Periodic Acid; C009288:metaperiodate; D006026:Glycoside Hydrolases