Facile formation of an intrastrand cross-link lesion between cytosine and guanine upon pyrex-filtered UV light irradiation of d((Br)CG) and duplex DNA containing 5-bromocytosine.
Pyrex-filtered UV light irradiation of d(BrCG) and 5-bromocytosine-containing duplex DNA leads to facile formation of a cross-link lesion between the C5 carbon atom of cytosine and the C8 carbon atom of its adjacent guanine. A similar cross-link lesion has been previously found in the X-ray irradiation mixture of d(CGTA).
Zeng
Yu
Y
Department of Chemistry-027, University of California at Riverside, Riverside, California 92521-0403, USA.
Wang
Yinsheng
Y
D000332:Aerobiosis; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000528:radiation effects; D003596:Cytosine / Q000031:analogs & derivatives* / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects; D004249:DNA Damage / Q000528:radiation effects; D006147:Guanine / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes; D009841:Oligonucleotides / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D014466:Ultraviolet Rays / Q000009:adverse effects
D003432:Cross-Linking Reagents; D009692:Nucleic Acid Heteroduplexes; D009841:Oligonucleotides; C103182:5-bromocytosine; D006147:Guanine; D003596:Cytosine; D004247:DNA