[Preparation and characterization of monoclonal antibodies against human transmembrane activator and calcium modulator and cyclophilin ligand interactor].
OBJECTIVE
To prepare monoclonal antibodies against human TACI and study the distribution of TACI on peripheral blood nuclear cells(PBMCs).


METHODS
Murine mAbs were prepared by hybridoma technique. The distribution of TACI on PBMCs were identified by immunofluorescence staining, FCM analysis as well as immunohistochemical staining.


RESULTS
Two hybridoma cell lines secreting mAbs against TACI were obtained. The titers of ascitic mAbs was 10(-7) and their Ig subclasses were IgG1. Both could recognize natural TACI on T cells. In the PHA activated models, TACI was expressed mainly on activated CD4(+) T cells.


CONCLUSIONS
Two mAbs can recognize natural TACI, which will be of greater value in research on the structure and functions of TACI.
Liu
Ying
Y
Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.
Liu
Xue-Song
XS
Zhu
Yong
Y
Chen
Li-Hua
LH
Cao
Yun-Xin
YX
Xu
Xiao-Guang
XG
Hu
Yi
Y
Tian
Ying
Y
Jin
Bo-Quan
BQ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology; D002118:Calcium / Q000378:metabolism; D021983:Cyclophilins; D006801:Humans; D006825:Hybridomas; D008807:Mice, Inbred BALB C; D053303:Transmembrane Activator and CAML Interactor Protein
D000911:Antibodies, Monoclonal; D053303:Transmembrane Activator and CAML Interactor Protein; D021983:Cyclophilins; D002118:Calcium