title: ELVIRA HSV, a yield reduction assay for rapid herpes simplex virus susceptibility testing.
abstract: A colorimetric yield reduction assay, ELVIRA (enzyme-linked virus inhibitor reporter assay) HSV, was developed to determine the antiviral drug susceptibilities of herpes simplex virus (HSV). It uses an HSV-inducible reporter cell line. This simple and rapid assay has an objective readout, low inoculum size, and good reproducibility. The results correlate well with those of the plaque reduction assay.
authors.affiliation: Department of Virology, Eijkman-Winkler Center, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands. r.stranska@azu.nl
authors.forename: Ruzena
authors.initials: R
authors.lastname: Stránská
authors.forename: Rob
authors.initials: R
authors.lastname: Schuurman
authors.forename: David R
authors.initials: DR
authors.lastname: Scholl
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Jollick
authors.forename: Carl J
authors.initials: CJ
authors.lastname: Shaw
authors.forename: Caroline
authors.initials: C
authors.lastname: Loef
authors.forename: Merjo
authors.initials: M
authors.lastname: Polman
authors.forename: Anton M
authors.initials: AM
authors.lastname: van Loon
mesh_terms: D000212:Acyclovir / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology*; D003124:Colorimetry; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D017245:Foscarnet / Q000494:pharmacology; D017930:Genes, Reporter / Q000235:genetics; D007763:Lac Operon / Q000235:genetics; D018139:Simplexvirus / Q000187:drug effects* / Q000235:genetics; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D017245:Foscarnet; D000212:Acyclovir