title: [Characterization of Mycobacterium tuberculosis polypeptide antigen capable of stimulating the proliferation of human gammadeltaT cells].
abstract: OBJECTIVE
  To analyse biological properties of Mycobacterium tuberculosis polypeptide antigen(Mtb-Ag) that could stimulate the proliferation of human gammadeltaT cells.
  
  
  METHODS
  Mtb secretory proteins and Mtb-Ag treated via dialysis or with pronase were used to stimulate human PBMCs. After being cultured for 10 days, the phenotype of responsive cells was analysed by flow cytometry.
  
  
  RESULTS
  The proliferation stimulating activity to human gammadeltaT cells of dialysed Mtb-Ag did not decrease remarkably, whereas that of the pronase-treated Mtb-Ag reduced significantly. Moreover, the proliferative stimulating activity to gammadeltaT cells of Mtb-Ag was notably higher than Mtb secretory protein.
  
  
  CONCLUSIONS
  The component in Mtb-Ag that can stimulate the proliferation of human gammadeltaT cells is a non-secretory and protase-sensitive polypeptides with M(r) over 10 000.
authors.affiliation: Department of Immunology, Bengbu Medical College, Bengbu 233003,China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: He-Zuo
authors.initials: HZ
authors.lastname: Lu
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Hu
authors.forename: Bai-Qing
authors.initials: BQ
authors.lastname: Li
mesh_terms: D000942:Antigens, Bacterial / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation; D009169:Mycobacterium tuberculosis / Q000235:genetics; D010455:Peptides; D014376:Tuberculosis / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial; D010455:Peptides