Synthesis of marimastat and a marimastat conjugate for affinity chromatography and surface plasmon resonance studies.
Immobilized small-molecule inhibitors are suited for enrichment of biomolecules by affinity chromatography, as it is shown for metalloproteinases and an immobilizable derivative of the hydroxamate-type inhibitor marimastat. A new asymmetric synthesis of marimastat is presented that allows for site-specific attachment to a solid surface, e.g., a chromatography matrix or a surface plasmon resonance sensor chip. The latter technique is shown to be a valuable tool for the optimization of binding and elution conditons of biomolecules in affinity chromatography.
Jenssen
Kai
K
Department of Chemistry, University of Bielefeld, Universitätsstrasse 25, D-33615 Bielefeld, Germany.
Sewald
Katherina
K
Sewald
Norbert
N
D015374:Biosensing Techniques / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods; D006877:Hydroxamic Acids / Q000138:chemical synthesis / Q000737:chemistry; D015394:Molecular Structure; D020349:Surface Plasmon Resonance / Q000379:methods*; D013997:Time Factors
D006877:Hydroxamic Acids; C100342:marimastat