title: [Regulation by astrocytic ATP of synaptic transmission].
abstract: Originally ascribed to having only passive roles in the CNS, astrocytes are now known to have an active role in the regulation of synaptic transmission. Neuronal activity can evoke Ca(2+) transients in astrocytes and Ca(2+) transients in astrocytes can evoke changes in neuronal activity. The excitatory neurotransmitter glutamate has been shown to mediate such bi-directional communication between astrocytes and neurons. We demonstrate here that ATP, a primary mediator of intercellular Ca(2+) signaling among astrocytes, also mediates intercellular signaling between astrocytes and neurons in hippocampal cultures. Mechanical stimulation of astrocytes evoked Ca(2+) waves mediated by the release of ATP and activation of P2 receptors. Mechanically evoked Ca(2+) waves led to decreased excitatory glutamatergic synaptic transmission in an ATP-dependent manner. Exogenous application of ATP does not affect post-synaptic glutamatergic responses but decreased pre-synaptic exocytotic events. Finally, we show that astrocytes exhibit spontaneous Ca(2+) oscillations mediated by extracellular ATP and that inhibition of these Ca(2+) responses enhanced excitatory glutamatergic transmission. We therefore conclude that ATP released from astrocytes exerts tonic and activity-dependent down-regulation of synaptic transmission via pre-synaptic mechanisms.
authors.affiliation: Division of Pharmacology, National Institute of Health Sciences. skoizumi@nihs.go.jp
authors.forename: Schuichi
authors.initials: S
authors.lastname: Koizumi
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Inoue
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001253:Astrocytes / Q000502:physiology*; D002118:Calcium / Q000502:physiology; D018698:Glutamic Acid / Q000502:physiology; D006624:Hippocampus / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D009474:Neurons / Q000502:physiology; D009435:Synaptic Transmission / Q000502:physiology*
chemical_list: D018698:Glutamic Acid; D000255:Adenosine Triphosphate; D002118:Calcium