title: Recombinant Sendai virus expressing the G glycoprotein of respiratory syncytial virus (RSV) elicits immune protection against RSV.
abstract: Although RSV causes serious pediatric respiratory disease, an effective vaccine does not exist. To capture the strengths of a live virus vaccine, we have used the murine parainfluenza virus type 1 (Sendai virus [SV]) as a xenogeneic vector to deliver the G glycoprotein of RSV. It was previously shown (J. L. Hurwitz, K. F. Soike, M. Y. Sangster, A. Portner, R. E. Sealy, D. H. Dawson, and C. Coleclough, Vaccine 15:533-540, 1997) that intranasal SV protected African green monkeys from challenge with the related human parainfluenza virus type 1 (hPIV1), and SV has advanced to clinical trials as a vaccine for hPIV1 (K. S. Slobod, J. L. Shenep, J. Lujan-Zilbermann, K. Allison, B. Brown, R. A. Scroggs, A. Portner, C. Coleclough, and J. L. Hurwitz, Vaccine, in press). Recombinant SV expressing RSV G glycoprotein was prepared by using reverse genetics, and intranasal inoculation of cotton rats elicited RSV-specific antibody and elicited protection from RSV challenge. RSV G-recombinant SV is thus a promising live virus vaccine candidate for RSV.
authors.affiliation: St. Jude Children's Research Hospital, 332 N. Lauderdale St., Memphis, TN 38105, USA.
authors.forename: Toru
authors.initials: T
authors.lastname: Takimoto
authors.forename: Julia L
authors.initials: JL
authors.lastname: Hurwitz
authors.forename: Chris
authors.initials: C
authors.lastname: Coleclough
authors.forename: Cecilia
authors.initials: C
authors.lastname: Prouser
authors.forename: Sateesh
authors.initials: S
authors.lastname: Krishnamurthy
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhan
authors.forename: Kelli
authors.initials: K
authors.lastname: Boyd
authors.forename: Ruth A
authors.initials: RA
authors.lastname: Scroggs
authors.forename: Brita
authors.initials: B
authors.lastname: Brown
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Nagai
authors.forename: Allen
authors.initials: A
authors.lastname: Portner
authors.forename: Karen S
authors.initials: KS
authors.lastname: Slobod
mesh_terms: D000818:Animals; D007114:Immunization; D022261:Respiratory Syncytial Virus Vaccines / Q000276:immunology*; D029082:Sendai virus / Q000235:genetics; D016205:Sigmodontinae; D014614:Vaccines, Synthetic / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D022261:Respiratory Syncytial Virus Vaccines; D014614:Vaccines, Synthetic; D014764:Viral Proteins