Plant phenolics protect from bleomycin-induced oxidative stress and mutagenicity in Salmonella typhimurium TA102.
Antioxidants are deemed to be important against DNA damage and mutations induced by reactive oxygen species (ROS). An assay for the ability of plant phenolics to protect against mutations caused by bleomycin treatment in Salmonella typhimurium TA102 cells in concentrations up to 20 microM was developed. Caffeic acid, gallic acid, protocatechuic acid, ferulic acid and rutin hydrate in final concentrations of 0.5 to 20 microM were tested for their ability to protect TA102 cells from mutations caused by oxidative stress from bleomycin. The cut-off concentration of 20 microM was used because as a limit it is biologically meaningful, higher concentrations being unrealistic in vivo. Caffeic acid was very potent at a concentration of 0.5-20 microM. The other four antioxidants were not effective up to 20 microM. The above assay will be helpful to characterize antioxidant molecules.
Stagos
Demetrios
D
Department of Biochemistry-Biotechnology, University of Thessaly, Larissa, Greece.
Kouris
Spyridon
S
Kouretas
Demetrios
D
D000964:Antimetabolites, Antineoplastic / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology*; D001761:Bleomycin / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D002109:Caffeic Acids / Q000494:pharmacology; D003373:Coumaric Acids / Q000494:pharmacology; D004347:Drug Interactions; D005707:Gallic Acid / Q000494:pharmacology; D062385:Hydroxybenzoates / Q000494:pharmacology; D009152:Mutagenicity Tests; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D012431:Rutin / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
D000964:Antimetabolites, Antineoplastic; D000975:Antioxidants; D002109:Caffeic Acids; D003373:Coumaric Acids; D062385:Hydroxybenzoates; D010636:Phenols; D010936:Plant Extracts; D001761:Bleomycin; C009091:protocatechuic acid; D012431:Rutin; D005707:Gallic Acid; C004999:ferulic acid; C040048:caffeic acid