Sympathetic innervation of white adipose tissue and its regulation of fat cell number.
White adipose tissue (WAT) is innervated by the sympathetic nervous system (SNS), and the central origins of this innervation have been demonstrated for inguinal and epididymal WAT (iWAT and eWAT, respectively) using a viral transneuronal tract tracer, the pseudorabies virus (PRV). Although the more established role of this sympathetic innervation of WAT is as a major stimulator of lipid mobilization, this innervation also inhibits WAT fat cell number (FCN); thus, local denervation of WAT leads to marked increases in WAT mass and FCN. The purpose of this study was to extend our understanding of the SNS regulation of FCN using neuroanatomical and functional analyses. Therefore, we injected PRV into retroperitoneal WAT (rWAT) to compare the SNS outflow to this pad from what already is known for iWAT and eWAT. In addition, we tested the ability of local unilateral denervation of rWAT or iWAT to promote increases in WAT mass and FCN vs. their contralateral neurally intact counterparts. Although the overall pattern of innervation was more similar than different for rWAT vs. iWAT or eWAT, its SNS outflow appeared to involve more neurons in the suprachiasmatic and solitary tract nuclei. Denervation produced significant increases in WAT mass and FCN for both iWAT and rWAT, but FCN was increased significantly more in iWAT than in rWAT. These data suggest differences in origins of the sympathetic outflow to WAT and functional differences in the WAT SNS innervation that could contribute to the differential propensity for fat cell proliferation across WAT depots in vivo.
Bowers
Robert R
RR
Molecular and Cellular Biology and Pathobiology Program, Medical University of South Carolina, Charleston, South Carolina 29425, USA.
Festuccia
William T L
WT
Song
C Kay
CK
Shi
Haifei
H
Migliorini
Renato H
RH
Bartness
Timothy J
TJ
D017667:Adipocytes / Q000502:physiology* / Q000648:ultrastructure; D000273:Adipose Tissue / Q000166:cytology* / Q000294:innervation*; D000818:Animals; D001921:Brain / Q000166:cytology; D001933:Brain Stem / Q000166:cytology; D002452:Cell Count; D048429:Cell Size; D006224:Cricetinae; D003714:Denervation; D011558:Herpesvirus 1, Suid; D008297:Male; D009929:Organ Size / Q000502:physiology; D017111:Phodopus; D016548:Prosencephalon / Q000166:cytology; D011557:Pseudorabies / Q000473:pathology; D013564:Sympathetic Nervous System / Q000502:physiology*