Endothelial nitric oxide synthase phosphorylated at Ser116 is localized in nerve fibers of the rat glandulae palatinae.
It is known that total endothelial nitric oxide synthase (eNOS) is localized in peripheral nerve fibers, but the existence of the phosphorylation site/s of eNOS in peripheral nerve fibers is unknown. In the perfusion-fixed and decalcified sections of rat palates, eNOS and eNOS phosphorylated at Ser(116) were identified in the nerve fibers of the glandulae palatinae. The localization of eNOS phosphorylated at Ser(1177) and Thr(495) in nerve fibers was undetectable. It is concluded that eNOS is phosphorylated at Ser(116) in nerve fibers of the glandulae palatinae under basal conditions. The basal Ser(1177) and Thr(495) phosphorylation of eNOS are missing in nerve fibers of the glandulae palatinae.
Korkmaz
Yüksel
Y
Department of Operative and Preventive Dentistry and Endodontics, Heinrich-Heine-University of Düsseldorf, Moorenstrasse 5, 40225 Duesseldorf, Germany. yueksel.korkmaz@uni-duesseldorf.de
Bloch
Wilhelm
W
Addicks
Klaus
K
Baumann
Michael A
MA
Raab
Wolfgang H-M
WH
D000818:Animals; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D009412:Nerve Fibers / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III; D010159:Palate / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D012694:Serine / Q000378:metabolism*
D012694:Serine; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat