title: Molecular characterisation of five trypsin-like peptidase transcripts from the salmon louse (Lepeophtheirus salmonis) intestine.
abstract: Four novel trypsin-like S1A peptidase transcripts (LsTryp2-5) from the marine parasitic copepod Lepeophtheirus salmonis were characterised based on analyses of 1918 expressed sequence tags from two adult female libraries. In addition, one previously described salmon louse trypsin, LsTryp1, has been further characterised. The five peptidases possessed all residues typically found in trypsins in correct sequence contexts. Interestingly, two cysteine residues, possibly involved in a disulphide bridge not previously reported in trypsins are conserved in all louse trypsin sequences. Phylogenetic analyses showed that the five louse peptidases form a monophyletic group with other crustacean trypsins (Brachyurin Ts). Quantitative PCR analyses demonstrated increased transcript levels from planktonic to early host-attached stages and from preadult to sexually mature adult stages. Furthermore, sex-specific differences in transcription regulation were found. In situ hybridisation demonstrated that all five trypsin-like peptidases are transcribed throughout the undifferentiated midgut, indicating a digestive function. The sequence characteristics, histological localisation and transcript regulation suggest that LsTryp1-4 encode typical digestive trypsins. LsTryp5, however, showed some sequence and regulatory peculiarities that rendered its function less clear. Our findings support earlier suggestions for the function of the midgut cells and suggest the existence of an additional undifferentiated cell-type.
authors.affiliation: Institute of Marine Research, P.O. Box 1870, Bergen N-5817, Norway.
authors.forename: Bjørn Olav
authors.initials: BO
authors.lastname: Kvamme
authors.forename: Rasmus
authors.initials: R
authors.lastname: Skern
authors.forename: Petter
authors.initials: P
authors.lastname: Frost
authors.forename: Frank
authors.initials: F
authors.lastname: Nilsen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D033342:Copepoda / Q000201:enzymology* / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D015723:Gene Library; D006790:Host-Parasite Interactions / Q000235:genetics; D017403:In Situ Hybridization / Q000379:methods; D007422:Intestines / Q000201:enzymology*; D008018:Life Cycle Stages / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012697:Serine Endopeptidases / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics; D014357:Trypsin
chemical_list: D018076:DNA, Complementary; D012697:Serine Endopeptidases; D014357:Trypsin