[Combined analysis on morphology, immunology, cytogenetics and molecular biology (MICM) classification of 55 patients with acute promyelocytic leukemia].
To investigate the value of bone marrow morphology, immunology, cytogenetics and molecular biology (MICM) examination in the diagnosis of acute promyelocytic leukemia (APL) and their relations of each other, the MICM data of 55 APL patients were analyzed retrospectively. The result showed that the accuracy rate of morphological diagnosis based on FAB classification could reach 96.4%; CD33 and CD13 antigen were co-expressed the highest in immunophenotyping (CD33(+)CD13(+) occupied 96.4%); cytogenetic abnormality containing t (15; 17)(q22; 21) accounted for 87.3%, translocation of chromosomes (simple type) of 100% t (15; 17)(q22; 21) occupied 75%, other involved chromosomes included 1, 8, 9, 11, 12, 21; the positive rate of PML/RARalpha gene also reached 96.4%; the accuracy rate of APL diagnosis by combining MICM measure was 100%. In conclusion, the bone marrow morphology still remains to be base for diagnosis of APL, but the combined analysis of MICM could obviously enhance the accuracy of diagnosis for APL. The MICM examination may provide a new approach to find subtype of APL.
Xiao
Rong
R
Jiangsu Institute of Hematology, The First Affiliated Hospital of Suzhou University, Suzhou 215006, China.
Zhang
Ri
R
D000293:Adolescent; D000328:Adult; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D015473:Leukemia, Promyelocytic, Acute / Q000145:classification* / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics; D015514:Oncogene Proteins, Fusion / Q000235:genetics
D009363:Neoplasm Proteins; D015514:Oncogene Proteins, Fusion; C093476:promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein