title: Development of the mammary gland requires DGAT1 expression in stromal and epithelial tissues.
abstract: Mammary gland development is a complex process that is dependent on interactions between the developing mammary epithelium and the surrounding stromal tissues. We show that mice lacking the triglyceride synthesis enzyme acyl CoA:diacylglycerol transferase 1 (DGAT1) have impaired mammary gland development, characterized by decreased epithelial proliferation and alveolar development, and reduced expression of markers of functional differentiation. Transplantation studies demonstrate that the impaired development results from a deficiency of DGAT1 in both the stromal and epithelial tissues. Our findings are the first to link defects in stromal lipid metabolism to impaired mammary gland development.
authors.affiliation: Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94141-1900, USA.
authors.forename: Sylvaine
authors.initials: S
authors.lastname: Cases
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Shillingford
authors.forename: Bryony S
authors.initials: BS
authors.lastname: Wiseman
authors.forename: Jo Dee
authors.initials: JD
authors.lastname: Fish
authors.forename: Christina S
authors.initials: CS
authors.lastname: Angle
authors.forename: Lothar
authors.initials: L
authors.lastname: Hennighausen
authors.forename: Zena
authors.initials: Z
authors.lastname: Werb
authors.forename: Robert V
authors.initials: RV
authors.lastname: Farese
mesh_terms: D000217:Acyltransferases / Q000096:biosynthesis* / Q000502:physiology*; D000818:Animals; D017209:Apoptosis; D002454:Cell Differentiation; D051048:Diacylglycerol O-Acyltransferase; D004847:Epithelial Cells / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D015151:Immunoblotting; D007150:Immunohistochemistry; D007774:Lactation; D050356:Lipid Metabolism; D008321:Mammary Glands, Animal / Q000196:embryology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011233:Precipitin Tests; D012313:RNA / Q000378:metabolism; D017154:Stromal Cells / Q000378:metabolism*; D013997:Time Factors; D016378:Tissue Transplantation
chemical_list: D012313:RNA; D000217:Acyltransferases; C494882:Dgat1 protein, mouse; D051048:Diacylglycerol O-Acyltransferase