title: Establishment and characterization of a primary androgen-responsive African-American prostate cancer cell line, E006AA.
abstract: BACKGROUND
  Establishment of human prostate cancer cell lines is essential to advance our understanding of complex processes associated with the initiation and progression of the disease. In the present study, we report the establishment of a primary African-American prostate cancer cell line (E006AA) as well as its associated stromal cells (S006AA).
  
  
  METHODS
  E006AA cell line was established as a spontaneously immortalized cells from a patient with a clinically localized prostate cancer. Extensive characterization of the cells was accomplished using androgen-dependent growth and sensitivity assays, Western analyses, RT-PCR/real-time PCR, cytogenetic analyses, and tumorigenicity in nude mice.
  
  
  RESULTS
  E006AA cell line shows androgen-dependent growth, expresses PSA and the androgen receptor (AR) with 26 CAG repeats in exon 1 of AR. Cytogenetic analyses revealed a hypertriploid karyotype with additional numerical gains in chromosomes 5, 6, 8, 10, 17, 20, 21 and a marker chromosome of unknown origin as well as structural abnormalities in chromosomes 4, 5, 8, 9, 11-14, 18, and 20. This cell line is not tumorigenic in nude mice. S006AA cell line, isolated from the same tumor specimen, also expresses AR and shows the morphological characteristics of smooth muscle cells of prostatic stroma.
  
  
  CONCLUSIONS
  These cell lines are the first available primary epithelial and stromal cells derived from an African-American patient with organ-confined prostate cancer and in conjunction with other established cell lines, could provide an in vitro model system to investigate early transforming events in prostate cancer.
authors.affiliation: Department of Biochemistry and Molecular Biology, Louisiana State University-Health Sciences Center, New Orleans, 70112, USA.
authors.forename: Shahriar
authors.initials: S
authors.lastname: Koochekpour
authors.forename: Grace A
authors.initials: GA
authors.lastname: Maresh
authors.forename: Adrienne
authors.initials: A
authors.lastname: Katner
authors.forename: Kitani
authors.initials: K
authors.lastname: Parker-Johnson
authors.forename: Tae-Jin
authors.initials: TJ
authors.lastname: Lee
authors.forename: Francine E
authors.initials: FE
authors.lastname: Hebert
authors.forename: Yuan S
authors.initials: YS
authors.lastname: Kao
authors.forename: John
authors.initials: J
authors.lastname: Skinner
authors.forename: Walter
authors.initials: W
authors.lastname: Rayford
mesh_terms: D001741:Black or African American; D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D018450:Disease Progression; D006801:Humans; D007621:Karyotyping; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D017430:Prostate-Specific Antigen / Q000032:analysis; D011471:Prostatic Neoplasms / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000728:Androgens; D017430:Prostate-Specific Antigen