title: [New molecular marker in dialysis setting--misfolded beta 2-microglobulin].
abstract: More than 25 clinical settings in amyloidosis have been acknowledged in which a peculiar criminal protein, a precursor protein, has been identified. As of now, however, the mechanism of amyloidogenesis, by which a precursor protein is transformed irreversibly into an amyloid protein, remains to be clarified. We speculated that a study of the molecular conformation of beta 2-microglobulin (beta 2 m), a precursor protein in dialysis-related amyloidosis (DRA), might provide a typic model of amyloidogenesis in other precursor proteins. Therefore, we investigated the misfolding of beta 2 m in DRA using a specific monoclonal antibody against C-terminal peptide 92-99 of beta 2 m. Our study indicated the possibility that the monoclonal antibody specific for C-terminal 92-99 of beta 2 m can detect a pre-amyloid state in amyloidogenesis in vivo, which might take place in the extravascular space.
authors.affiliation: Suiyukai Clinic, Kashihara 634-0007.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Motomiya
authors.forename: Katsuki
authors.initials: K
authors.lastname: Haraoka
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Morita
authors.forename: Izumi
authors.initials: I
authors.lastname: Amano
authors.forename: Xuguo
authors.initials: X
authors.lastname: Sun
authors.forename: Yukio
authors.initials: Y
authors.lastname: Ando
mesh_terms: D000686:Amyloidosis / Q000175:diagnosis* / Q000209:etiology*; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D006801:Humans; D011487:Protein Conformation; D017510:Protein Folding; D006435:Renal Dialysis / Q000009:adverse effects*; D001613:beta 2-Microglobulin / Q000737:chemistry / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D001613:beta 2-Microglobulin