Growth characteristics of porcine chlamydial strains in different cell culture systems and comparison with ovine and avian chlamydial strains.
Porcine Chlamydiaceae were cultivated under various culture conditions and we compared their growth characteristics with those of ruminant and avian strains. The combination of centrifugation assisted cell culture infection and cycloheximide treatment of Vero cell coverslip cultures provided the highest inclusion numbers with all chlamydial strains. Interestingly, the use of Iscove's modified Dulbecco's medium instead of Eagle's minimal essential medium significantly increased Chlamydia suis inclusion counts. C. suis and Chlamydophila pecorum inclusion numbers were markedly increased in CaCo cells, compared with Vero cells. This accelerated growth of porcine Chlamydiaceae under certain cultivation conditions may be helpful for the propagation of low chlamydial numbers or for their isolation from field samples. The intracellular distribution of porcine Chlamydiaceae in polarised CaCo cells clearly demonstrated differences between the chlamydial strains: C. pecorum 1710S inclusions were predominantly localised in the apical cytoplasm, C. suis S45 inclusions, however, were mostly situated in lower cytoplasmatic compartments. These findings might reflect biological differences in vivo.
Schiller
Irene
I
Institute of Veterinary Pathology, University of Zurich, Winterthurerstrasse 268, 8057 Zurich, Switzerland. schiller@vetpath.unizh.ch
Schifferli
Andrea
A
Gysling
Petra
P
Pospischil
Andreas
A
D000818:Animals; D018938:Caco-2 Cells / Q000382:microbiology; D018929:Cell Culture Techniques / Q000662:veterinary; D002689:Chlamydia / Q000145:classification* / Q000502:physiology; D002694:Chlamydiaceae Infections / Q000382:microbiology / Q000662:veterinary*; D002522:Chlorocebus aethiops; D010856:Columbidae / Q000382:microbiology; D006801:Humans; D012756:Sheep / Q000382:microbiology; D013552:Swine / Q000382:microbiology; D013553:Swine Diseases / Q000382:microbiology*; D014709:Vero Cells / Q000382:microbiology