What can we learn from highly purified neutrophils?
Neutrophil purification has traditionally been performed by centrifugation of leucocytes through density gradients. These reliable methods produce populations that are typically >95% pure neutrophils, and have allowed the widespread study of the function of these cells. Our recent work has suggested that residual monocytes may play a more important role than has been previously realized, and suggest that for some functional experiments, further purification of cells is required to understand fully the neutrophil phenotype.
Sabroe
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Division of Genomic Medicine, University of Sheffield, M Floor, Royal Hallamshire Hospital, Sheffield S10 2JF, UK.
Prince
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Dower
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Walmsley
S R
SR
Chilvers
E R
ER
Whyte
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MK
D000818:Animals; D017209:Apoptosis; D002469:Cell Separation; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D010641:Phenotype; D013997:Time Factors
D008070:Lipopolysaccharides