title: Chromosomal transformation in donor cells following allogeneic bone marrow transplantation.
abstract: We report here a monosomy 7 transformation of donor cells following matched-unrelated, same sex, allogeneic bone marrow transplantation in a patient with severe congenital aplastic anemia. A PCR technique was employed to amplify microsatellite markers on chromosome 7 to confirm donor/recipient identity. We found that the transformation of monosomy 7 occurred in previously genetically normal donor cells. This study suggests that the microenvironment of the bone marrow of our patient with severe congenital aplastic anemia may have played a critical role in the development of monosomy 7 of normal donor cells and we conclude that chromosomal microsatellite marker analysis can be a valuable tool for precise donor/recipient differentiation in engraftment monitoring.
authors.affiliation: Center for Cancer and Blood Disorders, Washington, DC, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Lang
authors.forename: P
authors.initials: P
authors.lastname: Dinndorf
authors.forename: S
authors.initials: S
authors.lastname: Ladisch
authors.forename: E
authors.initials: E
authors.lastname: Bayever
authors.forename: G
authors.initials: G
authors.lastname: Reaman
mesh_terms: D000741:Anemia, Aplastic / Q000150:complications / Q000151:congenital / Q000628:therapy*; D016026:Bone Marrow Transplantation; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002897:Chromosomes, Human, Pair 7; D020732:Cytogenetic Analysis; D005260:Female; D006801:Humans; D007223:Infant; D009006:Monosomy; D016133:Polymerase Chain Reaction; D014019:Tissue Donors; D014184:Transplantation, Homologous