title: [Development and application of an indirect immunofluorescence assay for the detection of serum immunoglobulin G to human herpesvirus 6].
abstract: OBJECTIVE
  To establish an indirect immunofluorescence assay(IFA) for detection of serum IgG antibody against human herpesvirus 6(HHV-6).
  
  
  METHODS
  Cord blood mononuclear cells infected by HHV-6 strain CN(5) were used to prepare cell antigen smear, so as to establish an IFA and make an epidemical investigation on serum specimens of child-bearing age, women.
  
  
  RESULTS
  The specificity of the IFA for HHV-6 was confirmed by absorption assay(test). The IFA detection showed that in serum specimens from 116 cases of child-bearing age, women, the positive rate of anti-HHV-6 IgG was 72.4% and geometric mean titer(GMT) was 1:61. The positive rate and GMT of serum anti-HHV-6 IgG had no differences between pregnant women and unpregnant women, neither among pregnant women at different pregnant stages.
  
  
  CONCLUSIONS
  A specific IFA has been developed successfully for epidemical investigation of HHV-6 infection rate in child-bearing women.
authors.affiliation: Department of Dermatology, 306th Hospital of PLA, Beijing 100101, China.
authors.forename: Jun-Lian
authors.initials: JL
authors.lastname: Liu
authors.forename: Zhi-Kai
authors.initials: ZK
authors.lastname: Xu
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Wang
authors.forename: De-Tang
authors.initials: DT
authors.lastname: Wang
authors.forename: Fang-Ling
authors.initials: FL
authors.lastname: Zhang
authors.forename: Qiao-Xin
authors.initials: QX
authors.lastname: Yang
authors.forename: Kun
authors.initials: K
authors.lastname: Yao
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D019084:Fluorescent Antibody Technique, Indirect; D006566:Herpesviridae Infections; D015654:Herpesvirus 6, Human; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G