T-588, a cognitive enhancer, protects against sodium nitroprusside-induced toxicity in cultured astrocytes.
The effects of (1R)-1-benzo[b]thiophen-5-yl-2-[2-(diethylamino)ethoxy]ethan-1-ol hydrochloride (T-588), a cognitive enhancer, on sodium nitroprusside (SNP)-induced cytotoxicity were examined in cultured rat astrocytes. Treatment with 100 microM SNP for 72 h decreased cell viability and mitochondrial function assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenil tetrazolium bromide (MTT) reduction activity, mitochondrial transmembrane potential, and intracellular ATP level. T-588 at 100 microM prevented SNP-induced mitochondrial dysfunction and cell injury. Furthermore, T-588 increased MTT reduction activity without affecting cell proliferation in astrocytes. These results suggest that T-588 has a protective effect against SNP-mediated toxicity via improvement of mitochondrial dysfunction in astrocytes.
Phuagphong
Patamawan
P
Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Japan.
Fukushima
Tetsuo
T
Hatanaka
Rie
R
Tanaka
Kazuhiro
K
Baba
Akemichi
A
Matsuda
Toshio
T
D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D004048:Diethylamines / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009599:Nitroprusside / Q000633:toxicity*; D018697:Nootropic Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013876:Thiophenes / Q000494:pharmacology*
D004048:Diethylamines; D018696:Neuroprotective Agents; D018697:Nootropic Agents; D013876:Thiophenes; C082372:T 588; D009599:Nitroprusside