title: Genomic characterization of thermophilic Geobacillus species isolated from the deepest sea mud of the Mariana Trench.
abstract: The thermophilic strains HTA426 and HTA462 isolated from the Mariana Trench were identified as Geobacillus kaustophilus and G. stearothermophilus, respectively, based on physiologic and phylogenetic analyses using 16S rDNA sequences and DNA-DNA relatedness. The genome size of HTA426 and HTA462 was estimated at 3.23-3.49 Mb and 3.7-4.49 Mb, respectively. The nucleotide sequences of three independent lambda-phage inserts of G. stearothermophilus HTA462 have been determined. The organization of protein coding sequences (CDSs) in the two lambda-phage inserts was found to differ from that in the contigs corresponding to each lambda insert assembled by the shotgun clones of the G. kaustophilus HTA426 genome, although the CDS organization in another lambda insert is identical to that in the HTA426 genome.
authors.affiliation: Microbial Genome Research Group, Japan Agency of Marine-Earth Science and Technology, 2-15 Natsushima, Yokosuka 237-0061, Japan, takamih@jamstec.go.jp
authors.forename: Hideto
authors.initials: H
authors.lastname: Takami
authors.forename: Shinro
authors.initials: S
authors.lastname: Nishi
authors.forename: Jei
authors.initials: J
authors.lastname: Lu
authors.forename: Shigeru
authors.initials: S
authors.lastname: Shimamura
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Takaki
mesh_terms: D001406:Bacillaceae / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005227:Fatty Acids / Q000378:metabolism; D005821:Genetic Techniques; D016680:Genome, Bacterial; D008957:Models, Genetic; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011809:Quinones / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013696:Temperature; D013729:Terpenes / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D013729:Terpenes; D004247:DNA