[Development of a rapid quantitative double mAb sandwich ELISA for detecting galactomannan antigen of aspergillus fumigatus].
OBJECTIVE
To develop a double mAb sandwich ELISA for rapidly and quantitatively detecting galactomannan (GM) antigen of aspergillus fumigatus.


METHODS
Four monoclonal antibodies (mAb) against GM of aspergillus fumigatus were used as coating or enzyme conjugated antibodies respectively. Capture and sandwich mAbs were selected by sandwich ELISA paired one by one.


RESULTS
Optimal capture and sandwich mAbs were selected and a highly sensitive double sandwich ELISA established. The sensitivity of detecting GM reached 0.1 microg/L, the detectable range was 0.1-10 microg/L. Coefficient of variation (C.V) obtained from detecting the same sample for 6 times by ELISA was (7.2+/-3.8)%.


CONCLUSIONS
A sensitive, repeatable and rapid double mAb sandwich ELISA was established for quantitation of aspergillus fumigatus GM, which might apply to early diagnosis and treatment of patients with aspergillosis.
Hao
Wei
W
Center of Laboratory, Zhujiang Hospital, First Military Medical University,Guangzhou 510282, China.
Qiu
Li-Wen
LW
Che
Xiao-Yan
XY
D000911:Antibodies, Monoclonal; D001228:Aspergillosis; D001232:Aspergillus fumigatus; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans
D000911:Antibodies, Monoclonal