title: Implantation of neural stem cells via cerebrospinal fluid into the injured root.
abstract: In avulsion injury of the dorsal root, regenerating axons cannot extend through the entry zone, i.e. the transition zone between peripheral and central nervous systems, due to the discontinuity between Schwann cells and astrocytes. We infused neural stem cells through the 4th ventricle in an attempt to enhance axonal growth in injured dorsal roots. Infused stem cells were attached to, and integrated into, the lesion of the root and became associated with axons in the same manner as Schwann cells or perineurial sheath cells in the peripheral nerve, and as astrocytes in the central nerve area. These findings suggest that neural stem cells integrated by infusion through CSF might have a beneficial effect on nerve regeneration by inducing a continuity of Schwann cells and astrocytes at the transition zone.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Kyoto University Graduate School of Medicine, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Ohta
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Toru
authors.initials: T
authors.lastname: Noda
authors.forename: Kazuya
authors.initials: K
authors.lastname: Kataoka
authors.forename: Hirotomi
authors.initials: H
authors.lastname: Chou
authors.forename: Namiko
authors.initials: N
authors.lastname: Ishikawa
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kitada
authors.forename: Naoya
authors.initials: N
authors.lastname: Matsumoto
authors.forename: Mari
authors.initials: M
authors.lastname: Dezawa
authors.forename: Shigehiko
authors.initials: S
authors.lastname: Suzuki
authors.forename: Chizuka
authors.initials: C
authors.lastname: Ide
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001253:Astrocytes / Q000502:physiology / Q000648:ultrastructure; D001369:Axons / Q000502:physiology / Q000648:ultrastructure; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D002555:Cerebrospinal Fluid / Q000166:cytology / Q000502:physiology; D004195:Disease Models, Animal; D005333:Fetus; D020546:Fourth Ventricle / Q000166:cytology / Q000502:physiology / Q000601:surgery; D006085:Graft Survival / Q000502:physiology; D007276:Injections, Intraventricular / Q000379:methods; D009416:Nerve Regeneration / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D010525:Peripheral Nerves / Q000254:growth & development / Q000648:ultrastructure; D011843:Radiculopathy / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000502:physiology / Q000648:ultrastructure; D013126:Spinal Nerve Roots / Q000254:growth & development* / Q000293:injuries* / Q000503:physiopathology; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology / Q000502:physiology; D013346:Subarachnoid Space / Q000166:cytology / Q000502:physiology; D016896:Treatment Outcome