title: Yap8p activation in Saccharomyces cerevisiae under arsenic conditions.
abstract: Yap8p, a member of the Saccharomyces cerevisiae Yap family, is activated in response to arsenic. Both the mechanisms by which this activation takes place and its regulation have not yet been identified. In this report, we show that Yap8p is not activated at the transcriptional level but, rather, its nuclear transport is actively regulated and dependent on the exportin chromosome region maintenance protein. In addition, it is shown that Cys(132), Cys(137)and Cys(274) are essential for Yap8p localization and transactivation function both of which are required for its biological activity.
authors.affiliation: Stress and Genomics Laboratory, Instituto de Tecnologia Química e Biológica, Avenida da República, Apt. 127, 2781-901 Oeiras, Portugal.
authors.forename: Regina A
authors.initials: RA
authors.lastname: Menezes
authors.forename: Catarina
authors.initials: C
authors.lastname: Amaral
authors.forename: Agnès
authors.initials: A
authors.lastname: Delaunay
authors.forename: Michel
authors.initials: M
authors.lastname: Toledano
authors.forename: Claudina
authors.initials: C
authors.lastname: Rodrigues-Pousada
mesh_terms: D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001151:Arsenic / Q000494:pharmacology*; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003545:Cysteine / Q000235:genetics; D017353:Gene Deletion; D005838:Genotype; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010641:Phenotype; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation / Q000187:drug effects
chemical_list: D002352:Carrier Proteins; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D003545:Cysteine; D001151:Arsenic