Immune responses to a GnRH-based anti-fertility immunogen, induced by different adjuvants and subsequent effect on vaccine efficacy.
A modified GnRH peptide (CHWSYGLRPG-NH2) was conjugated to tetanus toxoid and formulated with different adjuvants (non-ionic surfactant vesicles, aluminium hydroxide, Quil A, PLGA (poly(lactide-co-glycolide)/triacetin), and Quil A/PLGA). A comparison of the anti-fertility efficacy of the formulations was made by examining specific antibody levels, antibody subclasses, endocrine ablation and gonadal atrophy. The production of IgG2b antibody provided the best correlation for castration. PLGA was considered the most effective adjuvant as it produced a consistent anti-fertility response in all the treated animals.
Ferro
Valerie A
VA
Department of Immunology, University of Strathclyde, 27 Taylor Street, Glasgow G4 0NR, Scotland, UK. v.a.ferro@strath.ac.uk
Costa
Rosaria
R
Carter
K Christine
KC
Harvey
Michael J A
MJ
Waterston
Mary M
MM
Mullen
Alexander B
AB
Matschke
Christian
C
Mann
Jamie F S
JF
Colston
Angela
A
Stimson
William H
WH
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation; D007987:Gonadotropin-Releasing Hormone / Q000737:chemistry / Q000276:immunology*; D006062:Gonadotropins / Q000097:blood; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D009842:Oligopeptides / Q000737:chemistry / Q000276:immunology*; D009929:Organ Size / Q000187:drug effects / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013737:Testis / Q000187:drug effects / Q000276:immunology; D013739:Testosterone / Q000097:blood; D013745:Tetanus Toxoid / Q000737:chemistry; D022642:Vaccines, Contraceptive / Q000737:chemistry / Q000276:immunology*
D000276:Adjuvants, Immunologic; D006062:Gonadotropins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009842:Oligopeptides; C473153:TT-CHWSYGLRPG-NH2; D013745:Tetanus Toxoid; D022642:Vaccines, Contraceptive; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone