Recombination of fragmented proteins.
Directed evolution is a powerful method for generating novel molecules with desirable properties. In developing a new sensor to screen for protein-protein interactions, Tafelmeyer et al. report a clever strategy to evolve heterodimeric "split proteins" from a monomer in this issue of Chemistry & Biology.
Woycechowsky
Kenneth J
KJ
Laboratory of Organic Chemistry, Swiss Federal Institute of Technology, ETH Hönggerberg, CH-8093 Zurich, Switzerland.
D015374:Biosensing Techniques; D019281:Dimerization; D019020:Directed Molecular Evolution; D015723:Gene Library; D007370:Interferon Type I / Q000737:chemistry / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011257:Pregnancy Proteins / Q000737:chemistry / Q000378:metabolism; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism
D007370:Interferon Type I; D010446:Peptide Fragments; D011257:Pregnancy Proteins; D011506:Proteins; C020947:interferon tau