Construction and analysis of a subtracted library and microarray of cDNAs expressed specifically in chicken heart progenitor cells.
A subtracted library was constructed of genes expressed specifically in the chick precardiac mesoendoderm. The subtracted library was obtained by hybridization of nucleic acids derived from a starting tester library of stage 4-7 chick precardiac mesoendoderm and a starting driver library of stage 2 area pellucida. Approximately 11,000 clones from the resulting subtracted library were printed onto a microarray. Screening of the microarray with probes derived from cardiac and noncardiac tissues, followed by in situ hybridization during chick embryo development, has identified multiple cardiac-specific genes, including several that have not been characterized previously. The microarray will be useful for future attempts to identify additional novel cardiac-specific genes, as well as to characterize patterns of gene expression during heart differentiation.
Afrakhte
Mozhgan
M
Department of Molecular and Vascular Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA.
Schultheiss
Thomas M
TM
D000818:Animals; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D020869:Gene Expression Profiling; D015723:Gene Library; D006321:Heart / Q000196:embryology*; D017403:In Situ Hybridization; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
D018076:DNA, Complementary; D012333:RNA, Messenger