title: [Dynamic changes in the expressions of myogenic regulatory factors MyoD and myogenin during repair of muscle injury].
abstract: OBJECTIVE
  To observe the dynamic changes in the expressions of myogenic regulatory factors MyoD and myogenin during the repair of injured muscle.
  
  
  METHODS
  Muscular injury model was established by local injection of bupivacain, and at different time points following the injection, the gastrocnemius muscles were collected for preparation of cryosections. HE staining was performed for examination of the pathological changes in the injured muscles, and the expressions of MyoD and myogenin were detected by SABC.
  
  
  RESULTS
  MyoD-positive nuclei began to appear 18 h after muscle injury, reaching the peak till 48 h after the injury. Myogenin-positive nuclei appeared 24 h after muscle injury and peaked at 72 h.
  
  
  CONCLUSIONS
  MyoD and myogenin play a role in muscle regeneration after muscle injury, and they may serve as the indexes to identify muscle precursor cells and mark muscle regeneration process.
authors.affiliation: Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. drzengying@21cn.com
authors.forename: Ying
authors.initials: Y
authors.lastname: Zeng
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.forename: Ke-xuan
authors.initials: KX
authors.lastname: Liu
authors.forename: Cai-ming
authors.initials: CM
authors.lastname: Li
authors.forename: San-wei
authors.initials: SW
authors.lastname: Feng
authors.forename: Qun
authors.initials: Q
authors.lastname: Li
authors.forename: Tai-yun
authors.initials: TY
authors.lastname: Liu
authors.forename: Wen
authors.initials: W
authors.lastname: Huang
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002045:Bupivacaine / Q000633:toxicity*; D004268:DNA-Binding Proteins / Q000032:analysis; D064326:MEF2 Transcription Factors; D009132:Muscles / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology; D017570:MyoD Protein / Q000032:analysis*; D018006:Myogenic Regulatory Factors; D018008:Myogenin / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration; D014157:Transcription Factors / Q000032:analysis
chemical_list: D004268:DNA-Binding Proteins; D064326:MEF2 Transcription Factors; D017570:MyoD Protein; C503803:Myog protein, rat; D018006:Myogenic Regulatory Factors; D018008:Myogenin; D014157:Transcription Factors; D002045:Bupivacaine