Evaluation of pressurized liquid extraction and pressurized hot water extraction for tanshinone I and IIA in Salvia miltiorrhiza using LC and LC-ESI-MS.
Pressurized liquid extraction (PLE) and pressurized hot water extraction (PHWE) using a laboratory-made system are applied for the extraction of thermally labile components such as tanshinone I and IIA in Salvia miltiorrhiza. PLE and PHWE are carried out dynamically at a flow of 1 mL/min, temperature between 95-140 degrees C, applied pressure of 10-20 bars, and extraction times of 20 and 40 min, respectively. Effects of ethanol added into the water used in PHWE are explored. PLE is found to give comparable or higher extraction efficiencies compared with PHWE with reference to Soxhlet extraction for tanshinone I and IIA in Salvia miltiorrhiza. The tanshinone I and IIA present in the various medicinal plant extracts are determined by liquid chromatography and liquid chromatography-mass spectrometry.
Ong
Eng Shi
ES
Centre for Analytical Science, Health Sciences Authority, 11 Outram Road, Singapore 169078, Republic of Singapore. ong_eng_shi@hsa.gov.sg
Len
Shea Mei
SM
D045784:Abietanes; D002853:Chromatography, Liquid / Q000379:methods*; D006358:Hot Temperature; D010616:Phenanthrenes / Q000302:isolation & purification*; D011312:Pressure; D012015:Reference Standards; D027544:Salvia / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014867:Water
D045784:Abietanes; D010616:Phenanthrenes; C021751:tanshinone; D014867:Water