Effect of linoleic acid on proliferation and gene expression in the breast cancer cell line T47D.
Human and animal studies have linked n-6 polyunsaturated fatty acids with mammary carcinogenesis. We investigated the cellular and molecular effects of linoleic acid on the human breast cancer cell line T47D. Linoleic acid had a stimulatory effect on the growth of T47D cells, associated with an increase in the proportion of cells in the S phase of the cell cycle. Microarray, functional group and quantitative PCR analyses indicate that linoleic acid may affect T47D cell growth by modulation of the estrogen receptor (ERalpha), the G13alpha G protein, and p38 MAP kinase gene expression as well genes involved in RNA transcription and cell cycle regulation.
Reyes
Niradiz
N
Department of Microbiology and Immunology, New York Medical College, Valhalla, NY 10595, USA.
Reyes
Ismael
I
Tiwari
Raj
R
Geliebter
Jan
J
D002455:Cell Division; D045744:Cell Line, Tumor; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D047628:Estrogen Receptor alpha; D044365:GTP-Binding Protein alpha Subunits, G12-G13 / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019787:Linoleic Acid / Q000494:pharmacology*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D009841:Oligonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012313:RNA / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016196:S Phase; D014158:Transcription, Genetic; D015854:Up-Regulation; D048051:p38 Mitogen-Activated Protein Kinases
D047628:Estrogen Receptor alpha; D009841:Oligonucleotides; D011960:Receptors, Estrogen; D012313:RNA; D019787:Linoleic Acid; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D044365:GTP-Binding Protein alpha Subunits, G12-G13