[Study on the preparation of folate-conjugated albumin nanoparticles].
Bovine serum albumin nanoparticles(BSANP) were prepared by desolvation method. The activated folic acid (N-hydroxysuccinimide ester of folic acid) was conjuated to the surface of BSANP via the amino groups. Then the folate-conjugated BSANPs (folate-BSANP) were purified with Sephadex G-50 column and completely separated from unreacted folic acid. After chymotryptic hydrolysis, the extent of folate conjugation on the BSANP was determined by quantitative ultraviolet(UV) spectrophotometric analysis. It was found that the spectrum of trypsin digest of folate-conjugate BSANP is basically identical with that of folate, thus indicating folate is successfully expressed on the surface of BSANP. The folate-BSANP was averagely 66 nm in diameter and was spherical in shape. Folate-conjugated BSANP was achieved, which represents a potential new drug carrier for tumor cell-selective targeting.
Zhang
Liangke
L
West China School of Pharmacy, Sichuan University, Chengdu 610041.
Hou
Shixiang
S
Mao
Shengjun
S
Song
Xiangrong
X
Chen
Tong
T
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage; D002352:Carrier Proteins / Q000737:chemistry; D002417:Cattle; D016503:Drug Delivery Systems / Q000379:methods*; D058973:Folate Receptors, GPI-Anchored; D005492:Folic Acid / Q000737:chemistry*; D000922:Immunotoxins; D008863:Microspheres; D036103:Nanotechnology; D011956:Receptors, Cell Surface / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*
D000970:Antineoplastic Agents; D002352:Carrier Proteins; D058973:Folate Receptors, GPI-Anchored; D000922:Immunotoxins; D011956:Receptors, Cell Surface; D012710:Serum Albumin, Bovine; D005492:Folic Acid