title: Eight gram-negative bacteria are 10,000 times more sensitive to cationic detergents than to anionic detergents.
abstract: In liquid culture, eight typical gram-negative bacteria were ca. 10,000-fold more sensitive to cationic detergents than to the anionic detergent sodium dodecyl sulfate. Cetyltrimethylammonium bromide (CTAB) was inhibitory at concentrations ranging from 0.0006% to 0.01%. Four pseudomonads able to form biofilms were ca. 1000-fold more resistant to CTAB on Luria-Bertani agar plates than they were in liquid culture. A lasI mutant of Pseudomonas aeruginosa was only able to tolerate 0.1% CTAB on Luria-Bertani agar plates but could tolerate 5% CTAB when supplemented with homoserine lactone containing culture supernatants.
authors.affiliation: School of Biological Sciences, University of Nebraska, Lincoln, NE 68588-0666, USA.
authors.forename: Soumitra
authors.initials: S
authors.lastname: Rajagopal
authors.forename: Nicole
authors.initials: N
authors.lastname: Eis
authors.forename: Kenneth W
authors.initials: KW
authors.lastname: Nickerson
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000378:metabolism; D000838:Anions; D001548:Benzalkonium Compounds / Q000494:pharmacology; D002412:Cations; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology*; D002594:Cetylpyridinium / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology*; D016972:Enterobacter cloacae / Q000187:drug effects / Q000254:growth & development; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000254:growth & development; D011549:Pseudomonas / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D012706:Serratia marcescens / Q000187:drug effects / Q000254:growth & development; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
chemical_list: D000838:Anions; D001548:Benzalkonium Compounds; D002412:Cations; D002593:Cetrimonium Compounds; D003902:Detergents; C088386:homoserine lactone; D012967:Sodium Dodecyl Sulfate; D002594:Cetylpyridinium; D015107:4-Butyrolactone; D000077286:Cetrimonium