H3 mRNA level as a new proliferative marker in astrocytomas.
Replication-dependent H3.1 and H3.2 histones are encoded by 11 genes. The H3 mRNA levels in brain astrocytomas using real-time RT-PCR assay was examined. The sequence of primers and probe used in amplification was designed basing on the reference sequence GenBank accession no. The H3 mRNA levels correlated with tumor grade (R=0.56, P=0.0012), Ki-67 proliferative antigen labeling index (R=0.58, P=0.0008) and patient survival time (R=-0.50, P=0.005), discriminating low-grade and high-grade tumors. Quantification of H3 mRNA with real-time RT-PCR using the proposed pair of primers may supplement classic proliferative tests and predictive factors in brain astrocytomas.
Orchel
Joanna
J
Department of Molecular Biology, Medical University of Silesia, Narcyzow 1, 41-206, Sosnowiec, Poland.
Slowinski
Jerzy
J
Mazurek
Urszula
U
Wilczok
Tadeusz
T
D000328:Adult; D000368:Aged; D017398:Alternative Splicing; D001254:Astrocytoma / Q000235:genetics* / Q000473:pathology*; D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology*; D002455:Cell Division; D006657:Histones / Q000235:genetics*; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis / Q000378:metabolism; D008875:Middle Aged; D008969:Molecular Sequence Data; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
D006657:Histones; D019394:Ki-67 Antigen; D012333:RNA, Messenger