Differential transcription of beta-glucosidase and cellobiose dehydrogenase genes in cellulose degradation by the basidiomycete Phanerochaete chrysosporium.
Transcriptional analysis of beta-glucosidase gene (bgl) and cellobiose dehydrogenase gene (cdh) in relation to cellobiose metabolism in the basidiomycete Phanerochaete chrysosporium was performed using real-time quantitative RT-PCR. Addition of glucose to cellulose-degrading culture significantly decreased the number of both transcripts. In contrast, addition of cellobiose repressed only transcription of bgl but no effect for that of cdh. Moreover, to investigate induction of the two genes, the mycelia grown on glucose medium were transferred to medium containing glucose, cellobiose or no carbon source. In cellobiose medium, the number of bgl transcripts was slightly lower, whereas that of cdh transcripts was 2.3-fold higher than those in glucose medium. Consequently, cellobiose represses transcription of bgl, whereas it induces that of cdh.
Yoshida
Makoto
M
Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Igarashi
Kiyohiko
K
Kawai
Rie
R
Aida
Katsumi
K
Samejima
Masahiro
M
D002237:Carbohydrate Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D020075:Phanerochaete / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012331:RNA, Fungal / Q000378:metabolism; D014158:Transcription, Genetic; D001617:beta-Glucosidase / Q000235:genetics* / Q000378:metabolism
D012331:RNA, Fungal; D002244:Carbon; D002482:Cellulose; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase; D001617:beta-Glucosidase; D005947:Glucose