title: Control of pyrimidine biosynthesis in "Pseudomonas alkanolytica" ATCC 21034.
abstract: The control of de novo pyrimidine biosynthesis in the industrially important patent strain "Pseudomonas alkanolytica" ATCC 21034 was investigated. Uracil supplementation of succinate-grown "P. alkanolytica" cells produced the greatest depression of the de novo pyrimidine biosynthetic pathway enzyme activities. After the pyrimidine limitation of a "P. alkanolytica" orotate phosphoribosyltransferase mutant strain grown on succinate, the pyrimidine biosynthetic pathway enzyme activities were derepressed. The pyrimidine biosynthetic pathway enzyme aspartate transcarbamoylase in "P. alkanolytica" was inhibited by pyrophosphate, cytidine 5'-triphosphate (CTP), uridine 5'-triphosphate (UTP), and guanosine 5'-triphosphate (GTP).
authors.affiliation: Olson Biochemistry Laboratories, Department of Chemistry and Biochemistry, South Dakota State University, Brookings, SD 57007, USA. Thomas_West@sdstate.edu
authors.forename: Thomas P
authors.initials: TP
authors.lastname: West
mesh_terms: D001221:Aspartate Carbamoyltransferase / Q000378:metabolism; D003570:Cytidine Triphosphate / Q000378:metabolism; D011756:Diphosphates / Q000378:metabolism; D004794:Enzyme Repression; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006160:Guanosine Triphosphate / Q000378:metabolism; D009154:Mutation; D009962:Orotate Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D011743:Pyrimidines / Q000096:biosynthesis*; D019802:Succinic Acid / Q000378:metabolism; D014498:Uracil / Q000378:metabolism; D014544:Uridine Triphosphate / Q000378:metabolism
chemical_list: D011756:Diphosphates; D011743:Pyrimidines; D014498:Uracil; D003570:Cytidine Triphosphate; D006160:Guanosine Triphosphate; D019802:Succinic Acid; D001221:Aspartate Carbamoyltransferase; D009962:Orotate Phosphoribosyltransferase; C030986:pyrimidine; D014544:Uridine Triphosphate