The biological potency of a series of analogues of human calcitonin correlates with their interactions with phospholipids.
The conformational and lipid binding properties of several calcitonin analogs were compared. These analogs were designed to have the central amphipathic helical region of salmon calcitonin and N- and C-terminal segments similar to human calcitonin. The various analogs differed from one another either by removal of Leu19 from this hybrid analog, replacement of Leu19 with Gly19 or having a carboxyl terminus more closely related to salmon calcitonin. It had been found that replacement of Leu19 with Gly19 caused a marked reduction in the hypocalemic activity of the analog. The ability of the analogs to form helical structures in the presence of dimyristoylphosphatidylglycerol as well as their ability to lower the enthalpy of the calorimetric phase transition of this phospholipid correlates well with the hypocalcemic potency of the peptide.
Epand
Raquel F
RF
Department of Biochemistry, McMaster University, Hamilton, ON L8N 3Z5, Canada.
Orlowski
Ronald C
RC
Epand
Richard M
RM
D000595:Amino Acid Sequence; D000818:Animals; D002116:Calcitonin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D006996:Hypocalcemia / Q000139:chemically induced; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010715:Phosphatidylglycerols / Q000378:metabolism*; D011487:Protein Conformation; D013816:Thermodynamics
D010715:Phosphatidylglycerols; C028815:salmon calcitonin; D002116:Calcitonin; C002773:dimyristoylphosphatidylglycerol