title: The Butyrivibrio fibrisolvens tet(W) gene is carried on the novel conjugative transposon TnB1230, which contains duplicated nitroreductase coding sequences.
abstract: The Butyrivibrio fibrisolvens tet(W) gene is located on the conjugative transposon TnB1230. TnB1230 encodes transfer proteins with 48 to 67% identity to some of those encoded by Tn1549. tet(W) is flanked by directly repeated sequences with significant homology to oxygen-insensitive nitroreductases. The 340 nucleotides upstream of tet(W) are strongly conserved and are required for tetracycline resistance.
authors.affiliation: Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, United Kingdom.
authors.forename: Claire M
authors.initials: CM
authors.lastname: Melville
authors.forename: Romain
authors.initials: R
authors.lastname: Brunel
authors.forename: Harry J
authors.initials: HJ
authors.lastname: Flint
authors.forename: Karen P
authors.initials: KP
authors.lastname: Scott
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D045856:Butyrivibrio / Q000235:genetics*; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry; D020440:Gene Duplication; D008969:Molecular Sequence Data; D009601:Nitroreductases / Q000235:genetics*; D013753:Tetracycline Resistance / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D009601:Nitroreductases