Administration of phosphatidylcholine-cholesterol liposomes partially reconstitutes fat absorption in chronically bile-diverted rats.
OBJECTIVE
Intestinal bile deficiency in cholestatic patients leads to fat malabsorption. We addressed the potency of model bile, bile salts and phosphatidylcholine (PC)-cholesterol (CH) liposomes to reconstitute fat absorption in permanently bile-diverted (BD) rats.


METHODS
The plasma appearance of 13C-labeled palmitic acid (13C-16:0) and linoleic acid (13C-18:2) was determined after their enteral administration to BD or to control rats with an intact enterohepatic circulation (EHC) (13C-16:0 and 13C-18:2 dissolved in 25% olive oil-75% medium chain triacylglycerol oil mixture). BD rats were intraduodenally infused with buffer, model bile [consisting of 60 mM taurocholate (TC), 8 mM PC and 1 mM CH], buffer with TC, buffer with PC and CH liposomes, or buffer with lyso-PC and CH.


RESULTS
Plasma concentrations of 13C-16:0 and 13C-18:2 were consistently three- to eightfold higher in control rats than those in buffer-infused BD rats (P < 0.01). ID administration of either model bile or TC to BD rats restored plasma appearance of 13C-fatty acids at least to concentrations observed in control rats. Administration of PC + CH liposomes to BD rats partially reconstituted the plasma appearance of 13C-16:0, but did not affect that of 13C-18:2. Compared with control rats, the area under the curve (AUC) of plasma 13C-16:0 concentrations was 13.0 +/- 6.9% in buffer-infused rats and 40.9 +/- 3.1% in liposome-infused rats (P < 0.005).


CONCLUSIONS
Enteral administration of PC + CH liposomes to BD rats partially corrects the absorption of palmitic acid. Present data suggest that administration of PC + CH liposomes could enhance fat absorption in clinical conditions of cholestasis in which bile salt supplemention is contraindicated.
Nishioka
Tomoji
T
First Department of Internal Medicine, Hiroshima University School of Medicine, Hiroshima, Japan.
Having
Rick
R
Tazuma
Susumu
S
Stellaard
Frans
F
Kuipers
Folkert
F
Verkade
Henkjan J
HJ
D000818:Animals; D001652:Bile Ducts / Q000601:surgery*; D002784:Cholesterol / Q000008:administration & dosage*; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000378:metabolism; D007408:Intestinal Absorption; D008081:Liposomes; D008297:Male; D010713:Phosphatidylcholines / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar
D005227:Fatty Acids; D008081:Liposomes; D010713:Phosphatidylcholines; D002784:Cholesterol