title: [Construction of VEGF eukaryotic expression vector and its expression in endothelial cells and cardiac myocytes in-vitro].
abstract: OBJECTIVE
  To study the feasibility of VEGF165 gene transfection to endothelial cells and cardiac myocytes in-vitro.
  
  
  METHODS
  The VEGF165 DNA fragment was inserted into a green fluorescent protein fusion vector, which was then transfected into endothelial cells and cardiac myocytes with lipofectamine.
  
  
  RESULTS
  The endothelial cells and cardiac myocytes were successfully transfected with pIRES2-EGFP-hVEGF165 gene, which was confirmed by fluoroscopy and immunohistochemistry.
  
  
  CONCLUSIONS
  VEGF can be expressed stably in the endothelial cells and cardiac myocytes, which might be useful for the gene therapy of myocardial ischemia.
authors.affiliation: Department of Ultrasound, Xijing Hospital, Fourth Military Medical University, Xi'an 710032,China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yu
authors.forename: Yun-Qiu
authors.initials: YQ
authors.lastname: Qian
authors.forename: Shao-Ying
authors.initials: SY
authors.lastname: Lu
authors.forename: Guang-Sheng
authors.initials: GS
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Ye
mesh_terms: D042783:Endothelial Cells / Q000378:metabolism; D056890:Eukaryota / Q000235:genetics; D032383:Myocytes, Cardiac / Q000378:metabolism; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: D042461:Vascular Endothelial Growth Factor A