title: Development and implementation of a database system to manage a large-scale mouse ENU-mutagenesis program.
abstract: A mouse ENU-mutagenesis program at RIKEN GSC has been initiated to conduct a large-scale, genome-wide, early- and late-onset phenotypic screen of mutant mice. We screened about a hundred mice every week with a comprehensive set of phenotype assays including behavioral tests based on a modified SHIRPA protocol, blood tests (both clinical biochemical testing and hemogram), and measurement of locomotor activity in their home cages. To manage the entire program, we developed a client/server architecture database system and named it MUSDB (Mutagenesis Universal Support DataBase). It manages mouse husbandry, mating protocols, procedures for ENU injection and phenotypic screens, phenotype inheritance tests, preservation of sperm and organs, and other materials generated during the program. We have implemented MUSDB in quite a large-scale system that includes 150 client computers. It has, helped reduce typographical errors and provided simple and efficient operation via its front-end user interface. It significantly contributed to the communication within and between workgroups in the program and in the accumulation of various phenotypic and inheritance data.
authors.affiliation: Mouse Functional Genomics Research Group, RIKEN GSC, 214 Maeda-cho, Totsuka-ku, 244-0804, Yokohama City, Kanagawa, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Masuya
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nakai
authors.forename: Hiromi
authors.initials: H
authors.lastname: Motegi
authors.forename: Norio
authors.initials: N
authors.lastname: Niinaya
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Kida
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Kaneko
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Aritake
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Suzuki
authors.forename: Jun
authors.initials: J
authors.lastname: Ishii
authors.forename: Koji
authors.initials: K
authors.lastname: Koorikawa
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Suzuki
authors.forename: Maki
authors.initials: M
authors.lastname: Inoue
authors.forename: Kimio
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Hideaki
authors.initials: H
authors.lastname: Toki
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Wada
authors.forename: Hideki
authors.initials: H
authors.lastname: Kaneda
authors.forename: Junko
authors.initials: J
authors.lastname: Ishijima
authors.forename: K Ryo
authors.initials: KR
authors.lastname: Takahashi
authors.forename: Osamu
authors.initials: O
authors.lastname: Minowa
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Noda
authors.forename: Shigeharu
authors.initials: S
authors.lastname: Wakana
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Gondo
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Shiroishi
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D019992:Databases as Topic; D005038:Ethylnitrosourea / Q000494:pharmacology*; D005260:Female; D008297:Male; D051379:Mice / Q000235:genetics*; D008817:Mice, Mutant Strains; D016296:Mutagenesis; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation; D010641:Phenotype
chemical_list: D009153:Mutagens; D005038:Ethylnitrosourea