[Study on the enantiomer separation of cetirizine dihydrochloride using proteinate- and amylose-based chiral stationary phase].
OBJECTIVE
To study the chromatographic behavior of cetirizine dihydrochloride on the proteinate- and amylose- based chiral stationary phases so as to optimizate the chromatographic condition of its enantiomers separation.


METHODS
When using amylose-based, alpha1-acid glycoprotein and ovomucoid protein chiral stationary phase, the mobile phase was hexane-isopropyl alcohol-alcohol-trifluoroacetic acid (430:45:25:1), acetonitrile-10 mmol x L(-1) phosphate buffer solution (adjusted to pH 7.0 with sodium hydroxide) (4:96) and acetonitrile-20 mmol x L(-1) KH, PO4 solution (adjusted to pH 7.0 with triethylamine) (12.7:87.3), respectively. The temperature of proteinate column was 25 degrees C. The detective wavelength was 230 nm.


RESULTS
The two enantiomers could be separated on the two kinds of chiral stationary phases without derivatization and the resolution was above 2.0. The methods developed on the two kinds of chiral stationary phases are accurate, sensitive and specific.


CONCLUSIONS
Both the proteinate- and amylose-based chiral stationary phases can be used to separate the enantiomers of cetirizine.
Zhang
Zhe-feng
ZF
College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China.
Yang
Geng-liang
GL
Liang
Gui-jian
GJ
Zhou
Yu
Y
Chen
Yi
Y
D000688:Amylose / Q000031:analogs & derivatives; D017332:Cetirizine / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D039563:Histamine H1 Antagonists, Non-Sedating / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D009961:Orosomucoid; D013237:Stereoisomerism
D039563:Histamine H1 Antagonists, Non-Sedating; D009961:Orosomucoid; D000688:Amylose; D017332:Cetirizine