Different strains of noncytopathic bovine viral diarrhea virus (BVDV) vary in their affinity for in vivo-derived bovine embryos.
Washing procedures (without trypsin treatment) recommended by the International Embryo Transfer Society (IETS) for use on in vivo-derived embryos effectively removed a cytopathic strain (NADL) of bovine viral diarrhea virus (BVDV) after artificial exposure. However, these washing procedures have not been evaluated using other isolates of BVDV, including representative non-cytopathic strains. Thus, the objective of this study was to evaluate the efficacy of the IETS procedures following artificial exposure of in vivo-derived bovine embryos to two different strains and biotypes of BVDV. One hundred and twenty-nine zona pellucida-intact (ZP-I) morulae and blastocysts (MB) and 56 non-fertile and degenerated (NFD) ova were collected 7 days following exposure to bulls from 32, BVDV-negative, superovulated cows. After collection, all MB and NFD ova were washed according to IETS standards. Subsequently, half of the MB and NFD ova were exposed for 1h to approximately 10(6)-cell culture infective doses (50% endpoint) per milliliter of viral strain SD-1, and the other half were exposed to the same concentration of CD-87. After exposure, groups of > or =3 and < or = 10 MB or NFD ova were washed using methods that met or exceeded IETS standards. Then, the washed groups were sonicated, and sonicate fluids were assayed for presence of virus using virus isolation and a reverse transcription nested polymerase chain reaction. No virus was detected in any group of MB or NFD ova that had been exposed to the CD-87 isolate. However, virus was detected in association with 50% of the groups of MB and 33% of the groups of NFD ova that had been exposed to the SD-1 isolate. Therefore, standard embryo-washing procedures recommended by the IETS are more effective for removal of some isolates of BVDV than for others. It remains to be determined if the quantity of a high-affinity isolate of BVDV associated with individual washed embryos would infect recipients via the intrauterine route. Further, it should be determined if an alternative embryo processing procedure, washing and trypsin treatment, would be more effective for removal of high-affinity isolates.
Waldrop
Julie G
JG
College of Veterinary Medicine, Auburn University, 100 McAdory Hall, Auburn, AL 36849, USA. waldrja@vetmed.auburn.edu
Stringfellow
David A
DA
Riddell
Kay P
KP
Galik
Patricia K
PK
Riddell
M Gatz
MG
Givens
M Daniel
MD
Carson
Robert L
RL
Brock
Kenny V
KV
D000818:Animals; D002417:Cattle / Q000196:embryology*; D001908:Diarrhea Viruses, Bovine Viral / Q000302:isolation & purification / Q000502:physiology*; D004622:Embryo, Mammalian / Q000821:virology*; D005260:Female; D008297:Male; D016133:Polymerase Chain Reaction; D013010:Sonication; D013045:Species Specificity; D020858:Tissue and Organ Harvesting / Q000662:veterinary