title: Evaluation of cellulose-binding domain fused to a lipase for the lipase immobilization.
abstract: A cellulose-binding domain (CBD) fragment of a cellulase gene of Trichoderma hazianum was fused to a lipase gene of Bacillus stearothermophilus L1 to make a gene cluster for CBD-BSL lipase. The specific activity of CBD-BSL lipase for oil hydrolysis increased by 33% after being immobilized on Avicel (microcrystalline cellulose), whereas those of CBD-BSL lipase and BSL lipase decreased by 16% and 54%, respectively, after being immobilized on silica gel. Although the loss of activity of an enzyme immobilized by adsorption has been reported previously, the loss of activity of the CBD-BSL lipase immobilized on Avicel was less than 3% after 12 h due to the irreversible binding of CBD to Avicel.
authors.affiliation: Department of Chemical Engineering, Yonsei University, Seoul 120-749, Korea.
authors.forename: Sangpill
authors.initials: S
authors.lastname: Hwang
authors.forename: Jungoh
authors.initials: J
authors.lastname: Ahn
authors.forename: Sumin
authors.initials: S
authors.lastname: Lee
authors.forename: Tai Gyu
authors.initials: TG
authors.lastname: Lee
authors.forename: Seungjoo
authors.initials: S
authors.lastname: Haam
authors.forename: Kangtaek
authors.initials: K
authors.lastname: Lee
authors.forename: Ik-Sung
authors.initials: IS
authors.lastname: Ahn
authors.forename: Joon-Ki
authors.initials: JK
authors.lastname: Jung
mesh_terms: D002480:Cellulase / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D002482:Cellulose / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000235:genetics; D001411:Geobacillus stearothermophilus / Q000201:enzymology* / Q000235:genetics; D006868:Hydrolysis; D008049:Lipase / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry; D015202:Protein Engineering / Q000379:methods*; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics
chemical_list: D004800:Enzymes, Immobilized; D000069463:Olive Oil; D010938:Plant Oils; D011993:Recombinant Fusion Proteins; D002482:Cellulose; D008049:Lipase; D002480:Cellulase