Regulation of phosphatidylcholine biosynthesis under salt stress involves choline kinases in Arabidopsis thaliana.
Increasing evidence suggests a major role for phosphatidylcholine (PC) in plant stress adaptation. The present work investigated the regulation of choline, PC and interconnected phosphatidylethanolamine biosynthesis in Arabidopsis thaliana L. as a function of cold- and salt- or mannitol-mediated hyperosmotic stresses. While PC synthesis is accelerated in both salt- and cold-treated plants, the choline kinase (CK) and phosphocholine cytidylyltransferase genes are oppositely regulated with respect to these abiotic treatments. Salt stress also stimulates CK activity in vitro. A possible regulatory role of CK in stimulating PC biosynthesis rate in salt-stressed plants is discussed.
Tasseva
Guergana
G
Physiologie Cellulaire et Moléculaire des Plantes, UMR CNRS 7632, Université Pierre et Marie Curie, 4, place Jussieu, 75252 Paris Cedex 05, France. guergana.tasseva@snv.jussieu.fr
Richard
Luc
L
Zachowski
Alain
A
D017360:Arabidopsis / Q000201:enzymology* / Q000378:metabolism; D015152:Blotting, Northern; D002250:Carbon Radioisotopes; D002797:Choline Kinase / Q000378:metabolism*; D003080:Cold Temperature; D003566:Cytidine Diphosphate Choline / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D015870:Gene Expression; D006982:Hypertonic Solutions / Q000494:pharmacology; D008353:Mannitol / Q000494:pharmacology; D009997:Osmotic Pressure; D010713:Phosphatidylcholines / Q000096:biosynthesis* / Q000235:genetics; D010714:Phosphatidylethanolamines / Q000096:biosynthesis; D012965:Sodium Chloride / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects
D002250:Carbon Radioisotopes; D006982:Hypertonic Solutions; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; C483858:phosphatidylethanolamine; D008353:Mannitol; D012965:Sodium Chloride; D003566:Cytidine Diphosphate Choline; D002797:Choline Kinase