Correlation of allergen-induced IL-5 and IL-13 production by peripheral blood T cells of asthma patients.
BACKGROUND
Helper T cells and T cell cytokines are implicated in allergic disorders such as asthma and atopic dermatitis (AD). We reported enhanced interleukin-5 (IL-5) production by peripheral blood T cells of asthmatic patients. Production of cytokines, particularly IL-5 and IL-13, by peripheral blood mononuclear cells (PBMC) obtained from bronchial asthma (BA) and AD patients was investigated in the present study.


METHODS
PBMC were cultured in the presence of either polyclonal activator, phorbol ester plus Ca2+ ionophore or Dermatophagoides farinae (Df) antigen. The resulting supernatants were assayed for IL-4, IL-5, and IL-13 by specific ELISAs.


RESULTS
IL-5 and IL-13 production in response to Df antigen is significantly higher in allergic groups compared to control subjects. IL-5 responses induced by Df antigen were strongly correlated with IL-13 responses.


CONCLUSIONS
In spite of IL-13 gene proximity to IL-4 gene, IL-5 production was more strongly correlated to IL-13 production in the BA group, suggesting a common control mechanism that regulates the IL-5 and IL-13 gene.
Hashimoto
Tomomi
T
Clinical Research Center for Allergy and Rheumatology, National Sagamihara Hospital, Kanagawa, Japan.
Akiyama
Kazuo
K
Kawaguchi
Hiroshi
H
Maeda
Yuji
Y
Taniguchi
Masami
M
Kobayashi
Noriaki
N
Mori
Akio
A
D000328:Adult; D000485:Allergens / Q000276:immunology; D039741:Antigens, Dermatophagoides / Q000276:immunology; D001249:Asthma / Q000276:immunology*; D002478:Cells, Cultured; D003876:Dermatitis, Atopic / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D018793:Interleukin-13 / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000096:biosynthesis; D015848:Interleukin-5 / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000378:metabolism*
D000485:Allergens; D039741:Antigens, Dermatophagoides; D018793:Interleukin-13; D015848:Interleukin-5; D015847:Interleukin-4