title: Crystallization and preliminary crystallographic analysis of the fusion core from two new zoonotic paramyxoviruses, Nipah virus and Hendra virus.
abstract: Highly conserved heptad-repeat (HR1 and HR2) regions in class I viral fusion (F) proteins, including the F protein from paramyxovirus, interact with each other post-fusion to form a six-helix bundle called a fusion core. Crystals of the fusion core of Nipah virus have been grown at 291 K using PEG 4000 as precipitant. The diffraction pattern of the crystal extends to 2.1 angstroms resolution at 100 K in-house. The crystals have unit-cell parameters a = 31.664, b = 31.725, c = 51.256 angstroms, alpha = 80.706, beta = 86.343, gamma = 65.812 degrees and belong to space group P1. Crystals of the fusion core of Hendra virus have also been grown at 291 K using PEG 4000 as precipitant. The diffraction pattern of the crystal extends to 2.0 angstroms resolution at 100 K in-house. A selenomethionine (SeMet) derivative of the HeV fusion core was overexpressed using the same Escherichia coli expression system and purified. The derivative crystals were obtained under similar conditions and three different wavelength data sets were collected to 2.0 angstroms resolution from the derivative crystal at BSRF (Beijing Synchrotron Radiation Facility). The crystals have unit-cell parameters a = 31.997, b = 31.970, c = 53.865 angstroms, alpha = 85.990, beta = 85.842, gamma = 68.245 degrees and belong to space group P1.
authors.affiliation: Laboratory of Structural Biology and MOE Laboratory of Protein Sciences, School of Life Sciences and Bio-Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Xu
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Lou
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Liu
authors.forename: David K
authors.initials: DK
authors.lastname: Cole
authors.forename: Nan
authors.initials: N
authors.lastname: Su
authors.forename: Lan
authors.initials: L
authors.lastname: Qin
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Bai
authors.forename: Zihe
authors.initials: Z
authors.lastname: Rao
authors.forename: George F
authors.initials: GF
authors.lastname: Gao
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism; D045404:Hendra Virus / Q000378:metabolism*; D006801:Humans; D045405:Nipah Virus / Q000378:metabolism*; D011092:Polyethylene Glycols; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D013696:Temperature; D014760:Viral Fusion Proteins / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D014760:Viral Fusion Proteins; D011092:Polyethylene Glycols