Immunocharacterization of the DNA puff BhC4-1 protein of Bradysia hygida (Diptera: Sciaridae).
The DNA puff BhC4-1 gene is amplified and highly expressed in the salivary gland of Bradysia hygida late larvae. Using affinity-purified polyclonal antibodies we have identified the product of the BhC4-1 gene as a 43 kDa polypeptide which is present in extracts of salivary glands from late fourth instar larvae and in the corresponding gland secretion, but not in glands from earlier stages. We also demonstrate that this protein is produced mainly in the S1 and S3 regions of the salivary gland, where BhC4-1 amplification levels are more pronounced and larger amounts of mRNA are produced. By immunoelectron microscopy the BhC4-1 protein was detected in secretory granules of the S1 and S3 regions, and localized in fibrous structures present in the saliva.
Monesi
N
N
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
Silva
J A
JA
Martins
P C M
PC
Teixeira
A B
AB
Dornelas
E C
EC
Moreira
J E
JE
Paçó Larson
M L
ML
D000818:Animals; D004175:Diptera / Q000378:metabolism*; D005260:Female; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D008297:Male; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D012333:RNA, Messenger / Q000378:metabolism; D012469:Salivary Glands / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D012471:Salivary Proteins and Peptides / Q000235:genetics / Q000378:metabolism*
D019476:Insect Proteins; D012333:RNA, Messenger; D012471:Salivary Proteins and Peptides; C095605:puff C4 protein, Bradysia hygida