Determination of albumin concentration by MIP-QCM sensor.
An MIP-QCM sensor able to detect micro-determine albumin concentrations was prepared by imprinting albumin with 3-dimethylaminopropyl methacrylamide-acrylate. The albumin MIP was coated on a QCM Au electrode. The adsorption characteristics of different electrodes, such as Au-OH, Au-COOH, Au-NH2 and Au electrodes, with albumin or mixture was examined. In the tetraethyleneglycol dimethacrylate crosslinking agent system, the adsorption capacity of different Au electrodes is in the order Au-OH > Au-COOH > Au-NH2 > Au. Additionally, the time taken to receive a steady-state frequency is in the order Au-NH2 < Au-OH < Au-COOH < Au. However, in the trimethylolpropane trimethacrylate crosslinking agent system, the adsorption capacity is in the order Au > Au-NH2 > Au-OH > Au-COOH electrode. Hence, the crosslinking agent had a significant effect on the MIP-QCM. On the adsorption selectivity, the albumin MIP-QCM exhibited higher response to albumin, the adsorption mass ratio of cytochrome c:lysozyme:albumin:myoglobin was 160:1:1942:30. On the other hand, in the non-MIP-QCM, the adsorption mass ratio of cytochrome c:lysozyme:albumin:myoglobin was 13:1:249:86. Additionally, a linear fitting was established and a clinical real sample was tested. This novel potential application of molecular imprinting to the recognition element of an MIP-QCM sensor appears to be promising.
Lin
Thong-Yueh
TY
Department of Environmental Engineering, Kun-Shan University of Technology, Yung-Kang City, Tainan Hsien 710, Taiwan, ROC.
Hu
Chih-Hsien
CH
Chou
Tse-Chuan
TC
D000327:Adsorption; D000418:Albumins / Q000032:analysis* / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D045424:Complex Mixtures / Q000032:analysis / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D011108:Polymers / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000032:analysis*; D014159:Transducers
D000418:Albumins; D020099:Coated Materials, Biocompatible; D045424:Complex Mixtures; D011108:Polymers; D012709:Serum Albumin