Derivatization of amino acids with N,N-dimethyl-2,4-dinitro-5-fluorobenzylamine for liquid chromatography/electrospray ionization mass spectrometry.
Derivatization, separation and identification of amino acids with a novel compound, N,N-dimethyl-2,4-dinitro-5-fluorobenzylamine (DMDNFB), using high-performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI-MS) was demonstrated. Compared to derivatization with 2,4-dinitrofluorobenzene (DNFB), DMDNFB-derivatized amino acids and dipeptides exhibit much larger ion current signals in the commonly used ESI positive mode, which was attributed to the introduction of the N,N-dimethylaminomethyl protonatable site.
Liu
Zhongfa
Z
Department of Chemistry, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
Minkler
Paul E
PE
Lin
De
D
Sayre
Lawrence M
LM
D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry*; D001596:Benzylamines; D002847:Chromatography, Agarose / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004139:Dinitrofluorobenzene; D007202:Indicators and Reagents; D012680:Sensitivity and Specificity; D012685:Sepharose / Q000031:analogs & derivatives*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods* / Q000706:statistics & numerical data
D000596:Amino Acids; D001596:Benzylamines; D007202:Indicators and Reagents; C020927:phenyl-sepharose; D012685:Sepharose; D004139:Dinitrofluorobenzene