title: Cloning, expression and partial characterization of a Haemaphysalis longicornis and a Rhipicephalus appendiculatus glutathione S-transferase.
abstract: The ticks Haemaphysalis longicornis and Rhipicephalus appendiculatus are important parasites worldwide. The current method for control of cattle ticks involves the use of chemicals. Nevertheless, parasite resistance is an ever increasing global problem. Glutathione S-transferases (GSTs) play a central role in detoxication of xenobiotic and endogenous compounds. Several authors have noted that an increase in GST activity is associated with resistance to insecticides and acaricides. In the present study, we report the cloning and expression of GST cDNAs from H. longicornis and R. appendiculatus. In addition, we determine the effect of three acaricides (ethion, deltamethrin and diazinon) on the enzymatic activity of rGSTs.
authors.affiliation: Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido, Japan.
authors.forename: I
authors.initials: I
authors.lastname: da Silva Vaz Jnr
authors.forename: S
authors.initials: S
authors.lastname: Imamura
authors.forename: K
authors.initials: K
authors.lastname: Ohashi
authors.forename: M
authors.initials: M
authors.lastname: Onuma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D003124:Colorimetry; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D003976:Diazinon / Q000633:toxicity; D004789:Enzyme Activation / Q000187:drug effects; D015870:Gene Expression; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism*; D007305:Insecticide Resistance / Q000235:genetics; D026863:Ixodidae / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009570:Nitriles; D009946:Organothiophosphorus Compounds / Q000633:toxicity; D011722:Pyrethrins / Q000633:toxicity; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D009570:Nitriles; D009946:Organothiophosphorus Compounds; D011722:Pyrethrins; C017180:decamethrin; C100038:ethion; D005982:Glutathione Transferase; D003976:Diazinon