The CD200 and CD200 receptor cell surface proteins interact through their N-terminal immunoglobulin-like domains.
CD200 (OX2) is a broadly distributed cell surface glycoprotein that interacts with a receptor on myeloid cells (CD200R) involved in regulation of macrophage function. Both CD200 and CD200R contain two Ig superfamily domains like many other leukocyte membrane proteins. Site-directed mutagenesis of CD200R showed that, like CD200, it interacted through its N-terminal domain. This indicated that the cell-cell interaction spans four Ig superfamily domains and this distance is similar to many interactions found between T cells and antigen-presenting cells. This suggests that this topology is also important in interactions of CD200 on a variety of cells with CD200R on myeloid cells, and comparable contact sites may be important mediating regulation in other cell-cell interactions. The mutagenesis showed that the binding involved the predicted GFCC' face of its N-terminal domain, like that of CD200, suggesting that the interaction evolved from a homotypic interaction.
Hatherley
Deborah
D
Sir William Dunn School of Pathology, University of Oxford, Oxford, GB.
Barclay
A Neil
AN
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D000954:Antigens, Surface / Q000276:immunology* / Q000378:metabolism; D016466:CHO Cells; D006224:Cricetinae; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D022423:Myeloid Cells / Q000276:immunology; D064446:Orexin Receptors; D011956:Receptors, Cell Surface
D015703:Antigens, CD; D000954:Antigens, Surface; C477337:CD200R1 protein, human; D007136:Immunoglobulins; D064446:Orexin Receptors; D011956:Receptors, Cell Surface; C042739:antigens, CD200