[The expression of Cyclin D1 modulated by somatotropin on human pancreas cancer cell lines Bxpc-3].
OBJECTIVE
To observe the growth effect of somatostapin on human pancreas cancer lines Bxpc-3.


METHODS
The Bxpc-3 pancreas cancer cells were treated with Somatotropin. The cells hyperplasia were detected by MTT and were observed apoptosis cells determinated quantitatively by TUNEL, quantify immune fluoresence double marked the proliferation cells and apoptosis cells, the expression of Cyclin D1 detected by immunohistochemical.


RESULTS
The growth effect of pancrea cancer cells were limited by 10(-7) M, 10(-8) M, 10(-9) M Somatotropin on 2 day. The limited effect was decreased from 3 day. The cells proliferation were increased by somotostapin on 4day to 5day. The relationship between the expression of Cyclin D1 and apoptosis was negative correlation and the cells hyperplasia was positive correlation in Bxpc-3 cell line.


CONCLUSIONS
From the cell study we knew the expression of Cyclin D1 reflected the prolefiration of pancreas cancer cells.
Li
Fei
F
Capital University of Medical Sciences Xuanwu Hospital, Beijing 100053, China.
Liu
Da-chuan
DC
Sun
Jia-bang
JB
D017209:Apoptosis / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D019938:Cyclin D1 / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D013006:Growth Hormone / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors
D019938:Cyclin D1; D013006:Growth Hormone