title: [Cloning, prokaryotic expression of LAIR and identification of their immunological reactivities].
abstract: OBJECTIVE
  To clone and express the leukocyte-associated immunoglobulin-like receptor (LAIR) and to identify the immune reactivity of LAIR-2 to anti-LAIR-1 specific monoclonal antibodies(mAb).
  
  
  METHODS
  Genes encoding LAIR-1 and LAIR-2 were cloned by RT-PCR from human peripheral blood mononuclear cells(PBMC) and two leukemia cell lines Jurkat and HL-60. The extracellular region gene of LAIR1 and LAIR-2 were inserted into vector pGEX-4T-3 expressing GST fusion protein, expressed on IPTG induction and purified through glutathione-sepharose 4B column. The immunological reactivity of expressed LAIR-2 to anti-LAIR-1 mAb was identified by indirect ELISA.
  
  
  RESULTS
  LAIR-1 and LAIR-2 cDNAs had been cloned and expressed. Five new LAIR-1 cDNA isoforms were cloned. Among them, two isoforms from HL-60 included LAIR-1 open reading frames (ORF) and three isoforms from Jurkat were LAIR-1 cDNA segments. The LAIR-1 and LAIR-2 showed different immunological reactivities.
  
  
  CONCLUSIONS
  The transcription, processing after transcription and expression of LAIRs may be related to disparities in individuals and disease status. The difference in immunological reactivity may be involved in their structure.
authors.affiliation: Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Xie
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Ouyang
authors.forename: Jiang-Nan
authors.initials: JN
authors.lastname: Xue
authors.forename: Xin-Hai
authors.initials: XH
authors.lastname: Zhang
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.forename: Ran
authors.initials: R
authors.lastname: Zhuang
authors.forename: Bo-Quan
authors.initials: BQ
authors.lastname: Jin
mesh_terms: D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D007963:Leukocytes, Mononuclear; D011993:Recombinant Fusion Proteins / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins