Crystallization and preliminary X-ray crystallographic analyses of EcoO109I and its complex with DNA.
EcoO109I is a type II restriction endonuclease that recognizes seven base pairs of the degenerate and discontinuous sequence RGGNCCY. The enzyme and its complex with DNA were successfully crystallized by the hanging-drop vapour-diffusion method using polyethylene glycols as precipitants. The crystal of EcoO109I belongs to space group I4, with unit-cell parameters a = b = 175.5, c = 44.6 angstoms, and that of the DNA complex belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 49.1, b = 71.8, c = 203.2 angstroms. Full sets of X-ray diffraction data from the enzyme and its complex with DNA were collected to 2.4 and 1.9 angstroms resolution, respectively.
Imasaki
Tsuyoshi
T
Graduate School of Integrated Science, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
Hashimoto
Hiroshi
H
Shimizu
Toshiyuki
T
Kato
Matsuri
M
Tsuda
Junko
J
Kita
Keiko
K
Sato
Mamoru
M
D002384:Catalysis; D018360:Crystallography, X-Ray / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry*; D004058:Diffusion; D004926:Escherichia coli / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D014961:X-Ray Diffraction
D011092:Polyethylene Glycols; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C045450:RGGNCCY-specific type II deoxyribonucleases