title: Preparation of collagen/calcium phosphate multilayer sheet using enzymatic mineralization.
abstract: The multilayer sheets (2-10 layers), which consisted of alternately cumulated collagen and calcium phosphate layers with the thickness of 6-8microm in each layer, were prepared. The inorganic layer was mineralized by means of an alkaline phosphatase-catalyzed hydrolysis of water-soluble phosphate esters in the presence of calcium ions. The calcium phosphate, which was formed on the collagen, was assayed as a mixture of hydroxyapatite (main) and amorphous calcium phosphate. The multilayer sheets were not only strong mechanically but also semitransparent and flexible in a dry state. Furthermore, the collagen/calcium phosphate multilayer sheets did not swell in water to keep the original morphology. As a scaffold, the sheets having the calcium phosphate layer on the top supported the attachment and growth of L929 fibroblast cells. The enzymatic mineralization and the collagen/calcium phosphate composite sheets were discussed in conjunction with physicochemical and biological properties.
authors.affiliation: Department of Bioapplied Chemistry, Graduate School of Engineering, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan. jamauchi@bioa.eng.osaka-cu.ac.jp
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Yamauchi
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Goda
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Takeuchi
authors.forename: H
authors.initials: H
authors.lastname: Einaga
authors.forename: Toshizumi
authors.initials: T
authors.lastname: Tanabe
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D004577:Electron Probe Microanalysis; D006868:Hydrolysis; D008855:Microscopy, Electron, Scanning
chemical_list: D002130:Calcium Phosphates; C519480:amorphous calcium phosphate; D003094:Collagen; C020243:calcium phosphate; D000469:Alkaline Phosphatase