title: HPLC-DAD-MS identification of bioactive secondary metabolites from Ferula communis roots.
abstract: A simple HPLC method was developed to distinguish between 'poisonous' and 'non-poisonous' chemotypes of Ferula communis. The method was performed on a C8 reverse phase analytical column using a binary eluent (aqueous TFA 0.01%-TFA 0.01% in acetonitrile) under gradient condition. The two chemotypes showed different fingerprints. The identification of five coumarins and eleven daucane derivatives by HPLC-diode array detection (HPLC-DAD) and HPLC-MS is described. A coumarin, not yet described, was detected.
authors.affiliation: Indena S.p.A. Research and Development Laboratories, Via Don Minzoni 6, 20090 Settala (MI), Italy. lolita.arnoldi@indena.com
authors.forename: Lolita
authors.initials: L
authors.lastname: Arnoldi
authors.forename: Mauro
authors.initials: M
authors.lastname: Ballero
authors.forename: Nicola
authors.initials: N
authors.lastname: Fuzzati
authors.forename: Andrea
authors.initials: A
authors.lastname: Maxia
authors.forename: Enrico
authors.initials: E
authors.lastname: Mercalli
authors.forename: Luca
authors.initials: L
authors.lastname: Pagni
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003374:Coumarins / Q000737:chemistry*; D005309:Ferula; D006801:Humans; D013058:Mass Spectrometry; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots; D017368:Protein Prenylation; D015203:Reproducibility of Results
chemical_list: D003374:Coumarins; D010936:Plant Extracts; C092340:ferulenol