Specific erythrocyte binding capacity and biological activity of Plasmodium falciparum-derived rhoptry-associated protein 1 peptides.
Rhoptry-associated protein 1 (RAP1) is a merozoite antigen within Plasmodium falciparum rhoptries as yet having no specific function described for it. Synthetic peptides spanning the RAP1 sequence were tested in erythrocyte binding assays to identify possible RAP1 functional regions. Five high activity binding peptides (HABPs) were identified; 26201, 26202, 26203 and 26204 spanned residues 461C-K540 within RAP1 Cys region, whilst 26188 (201T-Y220) was located in p67 amino terminal. The results showed that peptide binding was saturable, some HABPs inhibited in vitro merozoite invasion and specifically bound to a 72 kDa protein in red blood cell membrane. HABP possible function in merozoite invasion of erythrocytes is also discussed.
Curtidor
Hernando
H
Fundación Instituto de Inmunología de Colombia and Universidad Nacional de Colombia, Cra 50 No. 26-00, Bogota 571, Colombia.
Ocampo
Marisol
M
Tovar
Diana
D
López
Ramses
R
García
Javier
J
Valbuena
Jhon
J
Vera
Ricardo
R
Suárez
Jorge
J
Rodríguez
Luis E
LE
Puentes
Alvaro
A
Guzmán
Fanny
F
Torres
Elizabeth
E
Patarroyo
Manuel E
ME
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000378:metabolism*; D001345:Autoradiography; D001667:Binding, Competitive; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism* / Q000469:parasitology; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism* / Q000494:pharmacology; D010963:Plasmodium falciparum / Q000187:drug effects; D011485:Protein Binding; D015800:Protozoan Proteins / Q000378:metabolism*; D011869:Radioligand Assay
D000953:Antigens, Protozoan; D010455:Peptides; D015800:Protozoan Proteins; C065352:rhoptry associated protein, Plasmodium