Capillary coating for protein separation based on Si-O and Si-C covalent bond formation for capillary electrophoresis with laser-induced fluorescence detection.
Protein adsorption to capillary walls is one of the major complications in protein analyses with capillary electrophoresis (CE). Coating the capillary with different materials is used to reduce the adsorption. This chapter overviews different approaches used for capillary coating and concentrates on those utilizing Si-O and Si-C covalent bonds. The apparatus and methods are presented for capillary coating using Si-O and Si-C chemistry. Furthermore, procedures are described for monitoring the quality of coating.
Ahmadzadeh
Hossein
H
Department of Chemistry, University of Minnesota, Minneapolis, MN., USA.
Dovichi
Norman J
NJ
Krylov
Sergey
S
D000180:Acrylic Resins / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D005456:Fluorescent Dyes / Q000737:chemistry*; D007834:Lasers; D011506:Proteins / Q000302:isolation & purification*; D013499:Surface Properties
D000180:Acrylic Resins; D005456:Fluorescent Dyes; D011506:Proteins