title: Increased expression of resistin and tumour necrosis factor-alpha in pig adipose tissue as well as effect of feeding treatment on resistin and cAMP pathway.
abstract: OBJECTIVE
  Resistin, a novel hormone, is expressed in mouse, rat and human adipose tissue. Its resistance to insulin is controversial. We hypothesized that resistin has the similar roles like those of tumour necrosis factor-alpha (TNF-alpha), a putative mediator of insulin resistance, and they are in common involved in regulating lipid metabolism in vivo.
  
  
  METHODS
  We demonstrated TNF-alpha and resistin expression in different growth-stage adipose tissue of genetically lean and obese pigs using semiquantitative reverse transcriptase-polymerase chain reaction and Western blotting analysis. Moreover, we analysed the variations of resistin and some parameters associated with lipolysis pathway after body-weight reduction in feeding-restricted obese pigs. In addition, we localized resistin expression in the cell types from pig adipose tissue.
  
  
  RESULTS
  Overall, the expression of TNF-alpha and resistin in obese pigs was statistically higher than their lean counterparts, although this difference in resistin mRNA expression tailed off with age. In individual pigs, their levels increased progressively with age and degree of obesity. After feeding restriction, resistin expression was gradually decreased. Simultaneously, feeding restriction also resulted in a significant decrease of serum triglyceride and plasma cAMP, free fatty acids and insulin as well as fat adenyl cyclase and cAMP. In addition, in isolated pig adipocytes, our data showed that resistin was localized not only to adipocytes but also to other unidentified stromal vascular cells.
  
  
  CONCLUSIONS
  TNF-alpha and resistin are increased in obesity. Weight loss results in the decrease of resistin and lipolysis-associated parameters, suggesting the possible association between resistin and lipolysis pathway. Cell localization of resistin expression implies the possibility of other biological actions of resistin.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, PR China.
authors.forename: X D
authors.initials: XD
authors.lastname: Chen
authors.forename: T
authors.initials: T
authors.lastname: Lei
authors.forename: T
authors.initials: T
authors.lastname: Xia
authors.forename: L
authors.initials: L
authors.lastname: Gan
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Yang
mesh_terms: D000273:Adipose Tissue / Q000254:growth & development / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D004038:Diet, Reducing; D004435:Eating / Q000502:physiology; D005260:Female; D006729:Hormones, Ectopic / Q000378:metabolism*; D008297:Male; D009765:Obesity / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D052243:Resistin; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D013851:Thinness / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D015431:Weight Loss / Q000502:physiology
chemical_list: D006729:Hormones, Ectopic; D052243:Resistin; D014409:Tumor Necrosis Factor-alpha; D012313:RNA