title: Immobilization of cell adhesive RGD peptide onto the surface of highly porous biodegradable polymer scaffolds fabricated by a gas foaming/salt leaching method.
abstract: A cell adhesive peptide moiety, Gly-Arg-Gly-Asp-Tyr (GRGDY), was immobilized onto the surface of highly porous biodegradable polymer scaffolds for enhancing cell adhesion and function. A carboxyl terminal end of poly(D,L-lactic-co-glycolic acid) (PLGA) was functionalized with a primary amine group by conjugating hexaethylene glycol-diamine. The PLGA-NH2 was blended with PLGA in varying ratios to prepare films by solvent casting or to fabricate porous scaffolds by a gas foaming/salt leaching method. Under hydrating conditions, the activated GRGDY could be directly immobilized to the surface exposed amine groups of the PLGA-NH2 blend films or scaffolds. For the PLGA blend films, the surface density of GRGDY, surface wettability change, and cell adhesion behaviors were characterized. The extent of cell adhesion was substantially enhanced by increasing the blend ratio of PLGA-NH2 to PLGA. The level of an alkaline phosphatase activity, measured as a degree of cell differentiation, was also enhanced as a result of the introduction of cell adhesive peptides.
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejon 305-701, South Korea.
authors.forename: Jun Jin
authors.initials: JJ
authors.lastname: Yoon
authors.forename: Soon Ho
authors.initials: SH
authors.lastname: Song
authors.forename: Doo Sung
authors.initials: DS
authors.lastname: Lee
authors.forename: Tae Gwan
authors.initials: TG
authors.lastname: Park
mesh_terms: D020341:Absorbable Implants; D000469:Alkaline Phosphatase / Q000378:metabolism; D000586:Amination; D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology*; D049109:Cell Proliferation; D005450:Fluorescamine / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D019344:Lactic Acid / Q000737:chemistry*; D008567:Membranes, Artificial; D008856:Microscopy, Fluorescence; D009842:Oligopeptides / Q000032:analysis / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley; D013499:Surface Properties
chemical_list: D008567:Membranes, Artificial; D009842:Oligopeptides; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D005450:Fluorescamine; C047981:arginyl-glycyl-aspartic acid; D000469:Alkaline Phosphatase