[Effects of Shenqi compound recipe on cell cycle and apoptosis of ascites inducible hepatocarcinoma cell line H22].
OBJECTIVE
To observe the effects of Shenqi compound recipe (SQ) on cell cycle and apoptosis of hepatocarcinoma cell line H22 in-vitro and in-vivo.


METHODS
BALB/c mice bearing tumors were perfused with SQ for 10 days running. And then cytotoxic activity of mouse NK cells and macrophages and variation of cell cycle of H22 cells were observed. Inhibition of H22 cell proliferation and induction of the their apoptosis by SQ were analyzed by flow cytometry.


RESULTS
The tumor-suppre ssive rate of SQ was 65.68%. SQ could make H22 cells arrested at the S phase and induce their apoptosis.


CONCLUSIONS
SQ has significant anti-tumor effect both in-vitro and in-vivo. Its mechanism may be related to blocking of cell cycle and inducing apoptosis of H22 cells.
Li
Hong-Zhe
HZ
Department of Medical Microbiology, Dalian Medical University, Dalian 116027,China.
Fan
Xiao-Lei
XL
Li
Qing
Q
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001201:Ascites; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D008113:Liver Neoplasms