title: The ex vivo study of synergistic effects of polycyclic aromatic hydrocarbon, benzo(a)pyrene with ovalbumin on systemic immune responses by oral route.
abstract: The present study was undertaken in order to examine whether oral administration of soluble antigen together with one of polycyclic aromatic hydrocarbons (PAHs) which is present in diesel exhaust particles (DEPs) called benzo(a)pyrene (BP), induced the systemic immune response in mice or not. Mice were orally given 1mg of ovalbumin (OA), a common food allergen, every 3 days over a period of 15 days. The results showed that oral administration of OA plus BP produced anti-OA IgE antibodies in serum, whereas either OA or BP alone failed to show the antigen-specific IgE antibody production. Production of anti-OA IgE antibody, which is dependent on Th2 CD4(+) T cells, was seen in mice fed with combined OA and BP was significantly higher than that of other groups. The anti-OA antibody production was associated with marked secretion of the Th1 cytokines, IFN-gamma and IL-12p70 as well as the Th2 cytokines IL-4, and IL-10. These results suggest that BP may act as a mucosal adjuvant in the gut enhancing systemic Th1 and Th2 immune responses and might play a role in oral immunization and food allergy.
authors.affiliation: Department of Immunology, School of Medical Sciences, Tarbiat Modarres University, P.O. Box 14115-331, Tehran, Iran. kadkhoda@modares.ac.ir
authors.forename: Kamran
authors.initials: K
authors.lastname: Kadkhoda
authors.forename: Zahra
authors.initials: Z
authors.lastname: Pourpak
authors.forename: Ali
authors.initials: A
authors.lastname: Akbar Pourfathallah
authors.forename: Anoshiravan
authors.initials: A
authors.lastname: Kazemnejad
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000284:Administration, Oral; D000485:Allergens / Q000008:administration & dosage / Q000276:immunology*; D000818:Animals; D001564:Benzo(a)pyrene / Q000008:administration & dosage / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D004357:Drug Synergism; D007111:Immunity, Cellular / Q000187:drug effects* / Q000276:immunology; D007114:Immunization; D007073:Immunoglobulin E / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism
chemical_list: D000276:Adjuvants, Immunologic; D000485:Allergens; D016207:Cytokines; D001564:Benzo(a)pyrene; D007073:Immunoglobulin E; D010047:Ovalbumin