title: Differential gene induction in macrophage-like human cells by two types of Porphyromonas gingivalis: a microarray study.
abstract: Several studies have provided clinical evidence that FimA clonal variation may contribute to the periodontopathogenicity of Porphyromonas gingivalis (P.g.). We studied the gene expression profiling of the macrophage-like human cell line U937 after infection of two types of P.g. (fimA type I; Pg-I and fimA type II; Pg-II) using microarray. Of 1088 genes examined, 394 genes were detectable. Bioinformatics algorithms were used to analyze the detectable genes. Hierarchical clustering analysis showed that gene expression patterns of Pg-II and the control (no infection) were grouped together. K-means clustering grouped 79 genes into Pg-II dominance and 88 genes into Pg-I dominance. A large number of genes related to cell signaling, extracellular communication proteins, cell receptors (by ligands), protein turnover and cell adhesion receptors/proteins were grouped into clusters of Pg-I dominance. Our results indicate that compared with Pg-I, Pg-II induces a low host response as measured by its weak induction of gene expression.
authors.affiliation: Nihon University Graduate School of Dentistry, Tokyo, Japan.
authors.forename: Maiko
authors.initials: M
authors.lastname: Oshikawa
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Sugano
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Koshi
authors.forename: Kyoko
authors.initials: K
authors.lastname: Ikeda
authors.forename: Koichi
authors.initials: K
authors.lastname: Ito
mesh_terms: D016866:Bacteroidaceae Infections / Q000382:microbiology; D002450:Cell Communication / Q000235:genetics; D002478:Cells, Cultured; D036781:Fimbriae Proteins / Q000145:classification / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D006801:Humans; D016023:Integrins / Q000235:genetics; D008264:Macrophages / Q000382:microbiology*; D020411:Oligonucleotide Array Sequence Analysis; D018933:Pili, Sex / Q000145:classification / Q000235:genetics; D016966:Porphyromonas gingivalis / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D011506:Proteins / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics; D015533:Transcriptional Activation
chemical_list: D016023:Integrins; D011506:Proteins; D011956:Receptors, Cell Surface; C063055:fimbrillin; D036781:Fimbriae Proteins