title: Typing single-nucleotide polymorphisms in Toxoplasma gondii by allele-specific primer extension and microarray detection.
abstract: Genotyping is an important tool for epidemiological and population genetic studies in protozoan parasites. The most commonly used method for genotyping is polymerase chain reaction (PCR)-based restriction fragment length polymorphism (RFLP) analysis of single nucleotide polymorphisms (SNPs). However, PCR-RFLP analysis is labor intensive, and only a proportion of the SNPs are recognized by currently available restriction enzymes. Here, we have developed a more efficient microarray-based method to genotype SNPs in the protozoan parasite Toxoplasma gondii. This method is sensitive, accurate, and capable of analyzing multiple SNPs simultaneously in a high-throughput format.
authors.affiliation: Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Su
authors.forename: Christian
authors.initials: C
authors.lastname: Hott
authors.forename: Bernard H
authors.initials: BH
authors.lastname: Brownstein
authors.forename: L David
authors.initials: LD
authors.lastname: Sibley
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005819:Genetic Markers; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide; D014122:Toxoplasma / Q000235:genetics*
chemical_list: D017931:DNA Primers; D005819:Genetic Markers