Coenzyme Q10 and lipid-related gene induction in HeLa cells.
OBJECTIVE
Coenzyme Q10 is an antioxidant that may have a therapeutic role in cervical cancer.


METHODS
We investigated the cellular and molecular effects of 30 micromol/L Coenzyme Q10 in HeLa cells. Cell growth assays, fluorescence-activated cell sorting analyses, and Oil Red O staining were performed. Microarray experiments were performed in duplicate and analyzed on the basis of 2-fold changes in levels of gene expression.


RESULTS
Coenzyme Q10 inhibited cell growth and led to apoptosis. Microarray analysis showed that 264 sequences were altered over time, with enrichment in lipid-related genes. Enhanced lipid accumulation was confirmed with Oil Red O staining.


CONCLUSIONS
A lipid response to Coenzyme Q10 may affect mechanisms of growth inhibition in HeLa cells.
Gorelick
Constantine
C
Albert Einstein College of Medicine, Albert Einstein Cancer Center and Montefiore Medical Center Bronx, NY 10467, USA.
Lopez-Jones
Melissa
M
Goldberg
Gary L
GL
Romney
Seymour L
SL
Khabele
Dineo
D
D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D002455:Cell Division / Q000187:drug effects*; D003067:Coenzymes; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D015227:Lipid Peroxidation; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D012016:Reference Values; D012680:Sensitivity and Specificity; D015533:Transcriptional Activation; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002583:Uterine Cervical Neoplasms
D000975:Antioxidants; D003067:Coenzymes; D012333:RNA, Messenger; D014451:Ubiquinone; C024989:coenzyme Q10