title: Physiological difference between free and triglyceride-type conjugated linoleic acid on the immune function of C57BL/6N mice.
abstract: Previous studies have shown the physiological significance of dietary conjugated linoleic acid (CLA) in various experimental animals and in human beings. One of the important problems to better elucidate is the difference between triglyceride (TG) and free (FFA) dietary CLA. Here, using splenocytes, this study assesses how TG- and FFA-CLA modulate immunoglobulin and various cytokine productions. In this study, C57BL/6N mice were fed an experimental diet containing 0% CLA, 0.1 or 1% FFA-CLA, or 0.1 or 1% TG-CLA for 3 weeks. The production of immunoglobulin tended to be up-regulated by 1% FFA-CLA. As a result of protein array analysis using the supernatant from splenocytes cultured with no CLA, 1% FFA-CLA, and TG-CLA, some cytokine production was shown to be remarkably regulated by dietary FFA- and TG-CLA. A total of 32 cytokines were examined, and 11-14 produced cytokines that were 2-fold up-regulated as compared with control for FFA- or TG-CLA, respectively. Especially, the production of IL-9 and MCP-5 and other cytokines was remarkably up-regulated by both FFA- and TG-CLA. In addition, seven cytokines were 2-fold down-regulated by TG-CLA. These data show that there is a slight but significant difference between the functionalities of FFA- and TG-CLA.
authors.affiliation: Research and Development Division, Omu Milk Products Company, Ltd, 1-38-1 Shin katsudachi Omuta, Fukuoka 836-0895, Japan. masawo@agr.kyushu-u.ac.jp
authors.forename: Masao
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Takae
authors.initials: T
authors.lastname: Kitagawa
authors.forename: Hitomi
authors.initials: H
authors.lastname: Chujo
authors.forename: Nami
authors.initials: N
authors.lastname: Koyanagi
authors.forename: Eri
authors.initials: E
authors.lastname: Nishida
authors.forename: Mako
authors.initials: M
authors.lastname: Nakaya
authors.forename: Kazuma
authors.initials: K
authors.lastname: Yoshimi
authors.forename: Hidenori
authors.initials: H
authors.lastname: Maeda
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Nou
authors.forename: Toshio
authors.initials: T
authors.lastname: Iwata
authors.forename: Kanehide
authors.initials: K
authors.lastname: Ogita
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Tachibana
authors.forename: Koji
authors.initials: K
authors.lastname: Yamada
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D004041:Dietary Fats / Q000008:administration & dosage; D007109:Immunity; D007136:Immunoglobulins / Q000096:biosynthesis; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009929:Organ Size; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D014280:Triglycerides / Q000494:pharmacology / Q000502:physiology*; D015430:Weight Gain
chemical_list: D016207:Cytokines; D004041:Dietary Fats; D007136:Immunoglobulins; D044243:Linoleic Acids, Conjugated; D014280:Triglycerides