RCA combined nanoparticle-based optical detection technique for protein microarray: a novel approach.
Developing a readily available biosensor with excellent performances is the main focus of many research groups. Recently, major breakthroughs in miniaturization of molecular analysis have produced DNA and protein microarrays. The aim of our group is to develop a sensitive technique for analyzing signals on protein microarray by applying the surface plasmon resonance (SPR) method. This new detection technique for specific molecular binding utilizes rolling circles amplification (RCA) post-signal processing method [Nat. Genet. 19 (1998) 225-232] and optical visualization by nanogold particle-labeled molecules on a micro-structured chip surface. By covalent bonding of the RCA primer to the detection antibody guarantees that the linkage between the analyte and the amplified RCA product is maintained during the assay. Experimental results show that RCA has significantly enhanced sensitivity compared to conventional methods. This combination of an easily detectable signal with chip technology should have the potential to become a successful commercial application.
Hsu
Hsin-Yun
HY
Institute of Biomedical Engineering, College of Engineering, College of Medicine, National Taiwan University, No. 1, Sec. 1, Jen-Ai Road, Taipei 100, Taiwan, ROC.
Huang
Yi-You
YY
D004270:DNA, Circular / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D006046:Gold / Q000737:chemistry; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D055095:Optics and Photonics / Q000295:instrumentation*; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods
D004270:DNA, Circular; D011506:Proteins; D006046:Gold