[Modulating effect of anticonvulsive agents on Ca2+ dependent membrane fusion in cell-free model of neurosecretion].
The effect of antiepileptic drug ethosuximide and sodium valproat on fusion of synaptic vesicles with synaptosomal plasma membranes was studied in cell-free system. It was shown that ethosuximide and sodium valproat increases the rate of Ca(2+)-dependent fusion reaction in vitro. We have found that convulsant drugs pentylenetetrazol and picrotoxin did not fuse membrane components of the model system. Ethosuximide- and sodium valproat-provoked fusion of synaptic vesicles and plasma membranes of synaptosomes were suppressed by convulsant drugs pentylenetetrazol and picrotoxin.
Trykash
I O
IO
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv. trikash@biochem.kiev.ua
Humeniuk
V P
VP
Hromov
L O
LO
Syrovats'ka
L P
LP
D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000648:ultrastructure; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D002474:Cell-Free System; D003292:Convulsants / Q000494:pharmacology; D005013:Ethosuximide / Q000494:pharmacology; D008561:Membrane Fusion / Q000187:drug effects*; D051381:Rats; D013572:Synaptic Vesicles / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D013574:Synaptosomes / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D014635:Valproic Acid / Q000494:pharmacology
D000927:Anticonvulsants; D003292:Convulsants; D005013:Ethosuximide; D014635:Valproic Acid; D002118:Calcium