[Genetic variability of the nucleocapsid protein of the virus of the porcine reproductive and respiratory syndrome].
The porcine reproductive and respiratory syndrome (PRRS) is a contagious viral pathology caused by PRRS virus. There are 2 types of the above virus--the European and American ones. Distribution patterns of the PRRS virus were studied for Russia and Byelorussia. Above 700 porcine sera obtained from 32 households of 21 Russia's administrative regions and from 19 households of 6 Byelorussia's administrative regions were tested for presence of antibodies to the PRRS virus. Simultaneously, the samples were tested for virus presence by polymerase chain reaction (PCR). It was proven serologically that the PRRS virus is widespread in the territories of Russia and Byelorussia. Noteworthily, all field isolates found in Russia and Byelorussia belong to the European type. Not a single viral isolate of the American PRRS type was found. The nucleocapsid (N) recombinant protein was obtained on the basis of the Russian field isolate of the PRRS virus by using the E. coli. expression system. Finally, it was shown as possible to use the recombinant protein in indirect immune enzyme assay for the sake of detecting the antibodies to the PRRS virus.
Grebennikova
T V
TV
Ivanovsky's Research Institute of Virology, Research and Production Company NARVAK, Russia's Academy of Medical Sciences, Moscow.
Zaberezhnyĭ
A D
AD
Vlasova
A N
AN
Musienko
M I
MI
Sokolov
M A
MA
Grabovetskiĭ
V V
VV
Tsybezov
V V
VV
Bogdanova
V S
VS
Orliankin
B G
BG
Aliper
T I
TI
Nepoklonov
E A
EA
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D019084:Fluorescent Antibody Technique, Indirect; D014644:Genetic Variation; D019590:Nucleocapsid Proteins / Q000235:genetics*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000097:blood / Q000453:epidemiology / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000145:classification / Q000235:genetics* / Q000276:immunology; D002094:Republic of Belarus / Q000453:epidemiology; D012426:Russia / Q000453:epidemiology; D016036:Seroepidemiologic Studies; D013552:Swine
D000914:Antibodies, Viral; D019590:Nucleocapsid Proteins