Single molecule diffraction.
For solving the atomic structure of organic molecules such as small proteins which are difficult to crystallize, the use of a jet of doped liquid helium droplets traversing a continuous high energy electron beam is proposed as a means of obtaining electron diffraction patterns (serial crystallography). Organic molecules (such as small proteins) within the droplet (and within a vitreous ice jacket) may be aligned by use of a polarized laser beam. Iterative methods for solving the phase problem are indicated. Comparisons with a related plan for pulsed x-ray diffraction from single proteins in a molecular beam are provided.
Spence
J C H
JC
Department of Physics and Astronomy, Arizona State University, Tempe, Arizona 85287-1504, USA.
Doak
R B
RB
D004583:Electrons; D006371:Helium / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry*; D033362:Powder Diffraction / Q000295:instrumentation / Q000379:methods*; D014961:X-Ray Diffraction
D008565:Membrane Proteins; D006371:Helium