title: Recognition of HLA-A3 and HLA-A11 by KIR3DL2 is peptide-specific.
abstract: The recognition of MHC class I molecules by killer cell immunoglobulin-like receptors (KIR) is central to the control of NK cell function and can also modulate the CTL activation threshold. Among KIR receptors, KIR3DL2 is thought to interact with HLA-A3 and -A11, although direct evidence has been lacking. In this study, we show that HLA-A3 and -A11 tetramers specifically bind to KIR3DL2*001 transfectants and that this recognition is peptide-specific. Single amino acid substitutions in the nonamer peptide underline a critical role for residue 8 in recognition of KIR3DL2. However, the role of this interaction in vivo still remains to be established.
authors.affiliation: Faculty of Medicine, King Chulalongkorn Memorial Hospital, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Pokrath
authors.initials: P
authors.lastname: Hansasuta
authors.forename: Tao
authors.initials: T
authors.lastname: Dong
authors.forename: Hathairat
authors.initials: H
authors.lastname: Thananchai
authors.forename: Michael
authors.initials: M
authors.lastname: Weekes
authors.forename: Christian
authors.initials: C
authors.lastname: Willberg
authors.forename: Hatice
authors.initials: H
authors.lastname: Aldemir
authors.forename: Sarah
authors.initials: S
authors.lastname: Rowland-Jones
authors.forename: Veronique M
authors.initials: VM
authors.lastname: Braud
mesh_terms: D005434:Flow Cytometry; D015234:HLA-A Antigens / Q000276:immunology*; D059870:HLA-A11 Antigen; D015791:HLA-A3 Antigen / Q000276:immunology*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D010446:Peptide Fragments; D011487:Protein Conformation; D011971:Receptors, Immunologic / Q000276:immunology*; D054340:Receptors, KIR; D054347:Receptors, KIR3DL2; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014162:Transfection
chemical_list: D015234:HLA-A Antigens; D059870:HLA-A11 Antigen; D015791:HLA-A3 Antigen; C515084:KIR3DL2 protein, human; D010446:Peptide Fragments; D011971:Receptors, Immunologic; D054340:Receptors, KIR; D054347:Receptors, KIR3DL2