title: Development of an isotope-coded activity-based probe for the quantitative profiling of cysteine proteases.
abstract: Quantification studies of complex protein mixtures have been restricted mainly to whole cell extracts. Here we describe the synthesis of two sets of isotope-coded activity-based probes that allow quantitative functional proteomics experiments on the cathepsins.
authors.affiliation: Gorlaeus Laboratories, Leiden University, Einsteinweg 55, 2300 RA Leiden, The Netherlands.
authors.forename: Paul F
authors.initials: PF
authors.lastname: van Swieten
authors.forename: Rene
authors.initials: R
authors.lastname: Maehr
authors.forename: Adrianus M C H
authors.initials: AM
authors.lastname: van den Nieuwendijk
authors.forename: Benedikt M
authors.initials: BM
authors.lastname: Kessler
authors.forename: Michael
authors.initials: M
authors.lastname: Reich
authors.forename: Chung-Sing
authors.initials: CS
authors.lastname: Wong
authors.forename: Hubert
authors.initials: H
authors.lastname: Kalbacher
authors.forename: Michiel A
authors.initials: MA
authors.lastname: Leeuwenburgh
authors.forename: Christoph
authors.initials: C
authors.lastname: Driessen
authors.forename: Gijsbert A
authors.initials: GA
authors.lastname: van der Marel
authors.forename: Hidde L
authors.initials: HL
authors.lastname: Ploegh
authors.forename: Herman S
authors.initials: HS
authors.lastname: Overkleeft
mesh_terms: D003546:Cysteine Endopeptidases / Q000032:analysis / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods*; D007554:Isotopes / Q000138:chemical synthesis / Q000378:metabolism; D040901:Proteomics / Q000379:methods
chemical_list: D007554:Isotopes; D003546:Cysteine Endopeptidases