A novel mechanism for regulating clonal propagation of mouse ES cells.
Self-renewal and differentiation of embryonic stem (ES) cells are controlled by the combinatorial action of extracellular signals and regulation of gene expression. For characterizing the entire molecular mechanism governing these events, we first established a feeder- and serum-free culture system in which mouse ES cells could propagate in clonal density in keeping with proper pluripotency. Supplementation of peptide hormones such as adrenocorticotropic hormone (ACTH) is required to remove serum, and the key event in this phenomenon may be the inhibition of the adenylyl cyclase (AC) activity, as it replaces the effect of these peptides. Because ES cells themselves produce the same activity, the finding suggests a novel mechanism in which activation of AC restricts clonal propagation of pluripotent stem cells.
Ogawa
Kazuya
K
Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology, Minatojima-Minamimachi 2-2-3, Chuo-ku, Kobe 650-0047, Japan.
Matsui
Hisanori
H
Ohtsuka
Satoshi
S
Niwa
Hitoshi
H
D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000378:metabolism; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D003470:Culture Media; D016895:Culture Media, Serum-Free; D004789:Enzyme Activation; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000201:enzymology
D000067956:Adenylyl Cyclase Inhibitors; D003470:Culture Media; D016895:Culture Media, Serum-Free; D000324:Adrenocorticotropic Hormone; D000262:Adenylyl Cyclases