[Cloning and expression of immunoadjuvant molecule--CTB gene].
We cloned cholera toxin subunit B gene from 569B and M045 strain of Vibrio cholerae with polymerase chain reaction, constructed recombinant plasmid pCTB, and transformed pCTB into the prokaryotic cell strain JM109. The indentification was made by means of restriction enzyme analysis, polymerase chain reaction, DNA sequencing, SDS-polyacrylamine gel electrophoresis analysis and Western blot. The results indicate that we have amplified cholera toxin subunit B gene of 376 bp from Vibrio cholerae and hve constructed the recombinant plasmid pCTB, and we have affained the object amied at successful expression of 12 KD in the prokaryotic cell strain.
Wang
Tao
T
Department of Parasitology, West China Medical Center, Sichuan University, Chengdu 610041, China.
Chen
Jianping
J
Zhang
Lei
L
Wang
Yufang
Y
Ma
Ying
Y
D000276:Adjuvants, Immunologic / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002772:Cholera Toxin / Q000096:biosynthesis / Q000235:genetics*; D022121:Cholera Vaccines; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics
D000276:Adjuvants, Immunologic; D022121:Cholera Vaccines; D011993:Recombinant Fusion Proteins; D002772:Cholera Toxin