title: Beneficial effects of supplemental buffer and substrate on energy metabolism during small bowel storage.
abstract: Successful preservation of small bowel (SB) is closely correlated with the maintenance of cellular energetics. This study was designed to assess the ability of a modified UW solution supplemented with buffer and glucose to facilitate ATP production during cold storage. In part A, rats SB (n = 4) were flushed vascularly as follows: Group 1, UW solution (control); Group 2, HUW solution (UW+90 mM histidine). Inclusion of histidine resulted in a >3-fold increase in buffering capacity over the pH range 7.4-6.8. Positive effects of histidine on ATP and energy charge were apparent after 4-10h storage. Examination of the key regulatory enzyme, Phosphofructokinase (PFK), reflected a sustained activation was over 1-4h in the HUW group only. In part B, groups were vascularly flushed as follows: Group 1, HUW solution (control); Group 2, Group 1+20mM glucose; and Group 3, Group 2+luminal flush. Elevated ATP and total adenylates over 2-10h in Group 3 compared to control were a direct consequence of improved glycolytic activity. This data supports the hypothesis that tissue energetics can be significantly improved during cold storage using a histidine-buffered UW solution supplemented with carbohydrate substrate.
authors.affiliation: Surgical-Medical Research Institute, 1074 Dentistry-Pharmacy Building, University of Alberta, Edmonton, Alta., Canada T6G 2N8.
authors.forename: Payam
authors.initials: P
authors.lastname: Salehi
authors.forename: Jennifer
authors.initials: J
authors.lastname: Spratlin
authors.forename: Tze-Feng
authors.initials: TF
authors.lastname: Chong
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Churchill
mesh_terms: D000241:Adenosine; D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000493:Allopurinol; D000818:Animals; D002021:Buffers; D003080:Cold Temperature; D004734:Energy Metabolism / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D005978:Glutathione; D024981:Glycogen Phosphorylase / Q000378:metabolism; D006019:Glycolysis; D006639:Histidine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007328:Insulin; D007421:Intestine, Small / Q000378:metabolism*; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D025221:Phosphofructokinases / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D011887:Raffinose; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002021:Buffers; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D006639:Histidine; D000493:Allopurinol; D000255:Adenosine Triphosphate; D024981:Glycogen Phosphorylase; D025221:Phosphofructokinases; D011770:Pyruvate Kinase; D005978:Glutathione; D005947:Glucose; D000241:Adenosine; D011887:Raffinose