title: Ionizing radiation induces delayed hyperrecombination in Mammalian cells.
abstract: Exposure to ionizing radiation can result in delayed effects that can be detected in the progeny of an irradiated cell multiple generations after the initial exposure. These effects are described under the rubric of radiation-induced genomic instability and encompass multiple genotoxic endpoints. We have developed a green fluorescence protein (GFP)-based assay and demonstrated that ionizing radiation induces genomic instability in human RKO-derived cells and in human hamster hybrid GM10115 cells, manifested as increased homologous recombination (HR). Up to 10% of cells cultured after irradiation produce mixed GFP(+/-) colonies indicative of delayed HR or, in the case of RKO-derived cells, mutation and deletion. Consistent with prior studies, delayed chromosomal instability correlated with delayed reproductive cell death. In contrast, cells displaying delayed HR showed no evidence of delayed reproductive cell death, and there was no correlation between delayed chromosomal instability and delayed HR, indicating that these forms of genome instability arise by distinct mechanisms. Because delayed hyperrecombination can be induced at doses of ionizing radiation that are not associated with significantly reduced cell viability, these data may have important implications for assessment of radiation risk and understanding the mechanisms of radiation carcinogenesis.
authors.affiliation: Radiation Oncology Research Laboratory, Bressler Research Building, Room 7-002, University of Maryland, 655 W. Baltimore St., Baltimore, MD 21201-1559, USA. lhuan001@umaryland.edu
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.forename: Suzanne
authors.initials: S
authors.lastname: Grim
authors.forename: Leslie E
authors.initials: LE
authors.lastname: Smith
authors.forename: Perry M
authors.initials: PM
authors.lastname: Kim
authors.forename: Jac A
authors.initials: JA
authors.lastname: Nickoloff
authors.forename: Olga G
authors.initials: OG
authors.lastname: Goloubeva
authors.forename: William F
authors.initials: WF
authors.lastname: Morgan
mesh_terms: D043171:Chromosomal Instability / Q000528:radiation effects; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D011995:Recombination, Genetic / Q000528:radiation effects*; D012107:Research Design