Nesterenkonia halotolerans sp. nov. and Nesterenkonia xinjiangensis sp. nov., actinobacteria from saline soils in the west of China.
The taxonomic position of two Gram-positive strains, YIM 70084(T) and YIM 70097(T), isolated from hypersaline soils was determined by a polyphasic approach. Cells of strain YIM 70084(T) are motile cocci, whereas those of strain YIM 70097(T) are non-motile rods. The G+C contents of their DNA are 64.4 and 66.7 mol%. Both strains had chemotaxonomic markers typical of the genus Nesterenkonia and formed a coherent cluster with Nesterenkonia species in a phylogenetic inference based on 16S rDNA sequence analysis, exhibiting less than 97 % similarity to each other and to the other two type strains of the genus. Phylogenetic distinction and differences in the peptidoglycan type, composition of cell-wall sugars, phospholipid patterns, the major menaquinones and other phenotypic characteristics indicate that the strains under study represent two novel species, Nesterenkonia halotolerans sp. nov. (type strain YIM 70084(T)=CCTCC AA 001022(T)=DSM 15474(T)) and Nesterenkonia xinjiangensis sp. nov. (type strain YIM 70097(T)=CCTCC AA 001025(T)=DSM 15475(T)).
Li
Wen-Jun
WJ
The Key Laboratory for Microbial Resources of Ministry of Education, People's Republic of China, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, People's Republic of China.
Chen
Hua-Hong
HH
Department of Chemistry, Chuxiong Normal College, Chuxiong, Yunnan 675000, People's Republic of China.|The Key Laboratory for Microbial Resources of Ministry of Education, People's Republic of China, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, People's Republic of China.
Zhang
Yu-Qin
YQ
The Key Laboratory for Microbial Resources of Ministry of Education, People's Republic of China, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, People's Republic of China.
Schumann
Peter
P
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
Stackebrandt
Erko
E
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
Xu
Li-Hua
LH
The Key Laboratory for Microbial Resources of Ministry of Education, People's Republic of China, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, People's Republic of China.
Jiang
Cheng-Lin
CL
The Key Laboratory for Microbial Resources of Ministry of Education, People's Republic of China, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, People's Republic of China.
D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008835:Micrococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010457:Peptidoglycan; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride