Postattachment events associated with viral entry are necessary for induction of interferon-stimulated genes by human cytomegalovirus.
Utilizing a human cytomegalovirus-specific fusion inhibitor and an antiglycoprotein H antibody, we studied the role of virion fusion in interferon-stimulated gene (ISG) induction. Our results indicate that ISG induction does not occur when virion-mediated, post-high-affinity attachment events are inhibited by either reagent. Thus, virion-mediated postattachment events, such as fusion, are required for ISG induction.
Netterwald
James R
JR
Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, 225 Warren Streeet, Newark, NJ 07101-1709, USA.
Jones
Thomas R
TR
Britt
William J
WJ
Yang
Shao-Jun
SJ
McCrone
Ian P
IP
Zhu
Hua
H
D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000378:metabolism / Q000472:pathogenicity*; D005347:Fibroblasts; D005786:Gene Expression Regulation; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D008561:Membrane Fusion / Q000187:drug effects; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000378:metabolism; D014771:Virion / Q000378:metabolism
D011506:Proteins; D014759:Viral Envelope Proteins; C071440:glycoprotein H, Cytomegalovirus; C499358:glycoprotein H, Human cytomegalovirus; D007372:Interferons