Identifying gp85-regions involved in Epstein-Barr virus binding to B-lymphocytes.
Epstein-Barr virus lacking glycoprotein gp85 cannot infect B-cells and epithelial cells. The gp85 belongs to the molecular complex required for virus invasion of B-lymphocyte or epithelial cells. Moreover, there is evidence that gp85 is necessary for virus attachment to epithelial cells. Thirty-six peptides from the entire gp85-sequence were tested in epithelial and lymphoblastoid cell line binding assays to identify gp85-regions involved in virus-cell interaction. Five of these peptides presented high binding activity to Raji, Ramos, P3HR-1, and HeLa cells, but not to erythrocytes; Raji-cell affinity constants were between 80 and 140nM. Of these five peptides, 11435 ((181)TYKRVTEKGDEHVLSLVFGK(200)), 11436 ((201)TKDLPDLRGPFSYPSLTSAQ(220)), and 11438 ((241)YFVPNLKDMFSRAVTMTAAS(260)) bound to a 65kDa protein on Raji-cell surface. These peptides and antibodies induced by them (recognising live EBV-infected cells) inhibited Epstein-Barr virus interaction with cord blood lymphocytes. It is thus probable that gp85-regions defined by peptides 11435, 11436, and 11438 are involved in EBV invasion of B-lymphocytes.
Urquiza
Mauricio
M
Fundación Instituto de Inmunología de Colombia and Universidad Nacional de Colombia, Bogota, Colombia.
Suarez
Jorge
J
Lopez
Ramses
R
Vega
Erika
E
Patino
Helena
H
Garcia
Javier
J
Patarroyo
Manuel A
MA
Guzman
Fanny
F
Patarroyo
Manuel E
ME
D000818:Animals; D001402:B-Lymphocytes / Q000821:virology*; D015153:Blotting, Western; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002469:Cell Separation; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000821:virology; D005434:Flow Cytometry; D006367:HeLa Cells; D004854:Herpesvirus 4, Human / Q000378:metabolism*; D006801:Humans; D007136:Immunoglobulins / Q000737:chemistry; D007962:Leukocytes / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D014759:Viral Envelope Proteins / Q000737:chemistry*
D007136:Immunoglobulins; D010455:Peptides; D014759:Viral Envelope Proteins; C090195:glycoprotein 85, Epstein-Barr virus