title: [Interaction between p53 and HSP70 in human hepatocellular carcinoma tissues].
abstract: OBJECTIVE
  To investigate the interaction between p53 and heat shock protein70 (HSP70) in human hepatocellular carcinoma tissues.
  
  
  METHODS
  HSP70 and p53-double positive samples were screened by imunohistochemical staining from twelve human hepatocellular carcinoma tissues, and the tissue homogenate was prepared. Coimmunoprecipitation was performed by using either anti-HSP70 mAb or anti-p53 mAb. The immunoprecipitate was analysed by SDS-PAGE and Western blot.
  
  
  RESULTS
  Immunohistochemical staining detection showed that three samples out of twelve cases were double positive for both HSP70 and p53. p53 protein existed in the immunoprecipitate of anti-HSP70 mAb, while there was HSP70 protein in the immunoprecipitate of anti-p53 mAb.
  
  
  CONCLUSIONS
  HSP70 and p53 exist as a complex in human hepatocellular carcinoma tissues, which will provide a new route for studying the pathogenesis and immunotherapy of human hepatocellular carcinoma.
authors.affiliation: Department of Pathology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Chong-Wei
authors.initials: CW
authors.lastname: Cui
authors.forename: Shou-Jing
authors.initials: SJ
authors.lastname: Yang
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Liu
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Liu
mesh_terms: D006528:Carcinoma, Hepatocellular; D004591:Electrophoresis, Polyacrylamide Gel; D018840:HSP70 Heat-Shock Proteins; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D018840:HSP70 Heat-Shock Proteins; D016159:Tumor Suppressor Protein p53