Molecular cloning and characterization of three adult rat beta-globin gene promoters.
We analyzed the promoter regions of the adult rat beta (IIbeta, IIIbeta, and 0beta)-globin genes. The results indicated that (1) the activities of the minimal promoters of these three genes are proportional to the gene expression levels in vivo, and (2) erythroid-specific repressor regions are located immediately upstream of the minimal promoter sequences and are regulated by the same transcription factor.
Okumura
Satoshi
S
Department of First Internal Medicine, Nippon Medical School, 1-1-5 Sendagi, Bunkyo, Tokyo 113-8602, Japan.
Fujii
Hiroyuki
H
Inokuchi
Naomi
N
Watanabe
Michiaki
M
Nishino
Takeshi
T
Okazaki
Taro
T
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D015723:Gene Library; D005914:Globins / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051381:Rats; D014157:Transcription Factors; D014162:Transfection
D014157:Transcription Factors; D005914:Globins; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase