The effects of nitric oxide on striatal serotoninergic transmission involve multiple targets: an in vivo microdialysis study in the awake rat.
The role of endogenous nitric oxide (NO) in N-methyl-D-aspartate (NMDA)-induced modulation of serotonin (5-HT) release in the striatum of freely moving rats has been studied using microdialysis technique. NMDA-induced increase in 5-HT release was significantly inhibited by selective nitric oxide synthase (nNOS) inhibitor S-methylthiocitrulline (S-Me-TC), ONOO- scavenger L-cysteine (L-cys), and guanylate cyclase (GC) inhibitor 1H[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ). These data suggest that modulation of 5-HT levels is linked to the formation of NO produced by NMDA receptor activation and that endogenously produced NO increases 5-HT concentrations both by stimulating formation of 3'-5'-cyclic monophosphate (cGMP) and conversion of ONOO-.
Trabace
Luigia
L
Department of Biomedical Sciences, Faculty of Medicine c/o OO.RR., University of Foggia, Viale L. Pinto, Foggia 71100, Italy. trabace@unifg.it
Cassano
Tommaso
T
Tucci
Paolo
P
Steardo
Luca
L
Kendrick
Keith M
KM
Cuomo
Vincenzo
V
D000818:Animals; D006152:Cyclic GMP / Q000502:physiology; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers / Q000494:pharmacology; D006162:Guanylate Cyclase / Q000502:physiology; D008297:Male; D017551:Microdialysis; D016202:N-Methylaspartate / Q000502:physiology; D017072:Neostriatum / Q000187:drug effects* / Q000378:metabolism; D009569:Nitric Oxide / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D052248:Nitric Oxide Synthase Type I; D030421:Peroxynitrous Acid / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects; D013481:Superoxides / Q000378:metabolism; D009435:Synaptic Transmission / Q000187:drug effects*
D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D013481:Superoxides; D030421:Peroxynitrous Acid; D009569:Nitric Oxide; D012701:Serotonin; D016202:N-Methylaspartate; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat; D006162:Guanylate Cyclase; D006152:Cyclic GMP