title: Luminescent pH sensing and DNA binding properties of a novel ruthenium(II) complex.
abstract: A new Ru(II) complex, [Ru(bpy)(2)(dhipH3)](ClO4)(2) (in which bpy=2,2'-bipyridine, dhipH(3)=3,4-dihydroxy-imidado[4,5-f][1,10]-phenanthroline), was synthesized and characterized, and the pH effect on the emission spectra of the complex was studied. The interaction of the complex with calf thymus DNA was investigated by UV-visible and emission spectroscopy, and viscosity measurements. The results suggest that the complex acted as a sensitive luminescent pH sensor and a strong ct-DNA intercalator with an intrinsic binding constant of (4.0+/-0.7) x 10(5) M(-1) in buffered 50 mM NaCl.
authors.affiliation: Department of Chemistry, Beijing Normal University, Xinjiecouwai Street 19, Beijing 100875, China.
authors.forename: Guang-Yao
authors.initials: GY
authors.lastname: Bai
authors.forename: Ke-Zhi
authors.initials: KZ
authors.lastname: Wang
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Duan
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Gao
mesh_terms: D004247:DNA / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007364:Intercalating Agents / Q000138:chemical synthesis* / Q000737:chemistry*; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D007364:Intercalating Agents; D009942:Organometallic Compounds; D012428:Ruthenium; D004247:DNA