Constitutive expression of interleukin-18 and interleukin-18 receptor mRNA in tumour derived human B-cell lines.
Interleukin-18 (IL-18) is a pro-inflammatory cytokine involved in the Th1 immune response and expressed by a variety of cell types. IL-18 is a member of the IL-1 family and plays an important role in autoimmune diseases and inflammation. Using reverse transcriptase-polymerase chain reaction (RT-PCR) mRNA expression of IL-18, IL-18 receptor alpha (IL-18R alpha), and beta (IL-18R beta) were studied in tumour derived human B-cell lines. Furthermore, we investigated IL-18 protein secretion by using enzyme linked immunosorbent assay (ELISA). The results, as presented in this report, suggest that IL-18, IL-18R alpha, and IL-18R beta mRNA are constitutively and ubiquitously expressed in human B-cell lines, but secretion of the functional protein does not occur. We therefore speculate that IL-18 possibly affects B-cells through paracrine actions.
Lorey
S L
SL
Laboratory of Cytokine Research, Department of Biology, University of West Florida, Pensacola, Florida 32514, USA.
Huang
Y C
YC
Sharma
V
V
D001402:B-Lymphocytes / Q000276:immunology*; D045744:Cell Line, Tumor; D006801:Humans; D020382:Interleukin-18 / Q000235:genetics*; D053727:Interleukin-18 Receptor alpha Subunit; D012333:RNA, Messenger / Q000032:analysis*; D018123:Receptors, Interleukin / Q000235:genetics*; D053724:Receptors, Interleukin-18; D020133:Reverse Transcriptase Polymerase Chain Reaction
C508572:IL18R1 protein, human; D020382:Interleukin-18; D053727:Interleukin-18 Receptor alpha Subunit; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D053724:Receptors, Interleukin-18