[Effect of glia cell line-derived neurotrophic factor on cultured spinal motor neurons from embryonic rat].
OBJECTIVE
To observe the effects of various concentrations of gli-a cell line-derived neurotrophic factor ( GDNF) on the growth of cultured spinal motor neurons from embryonic rat.


METHODS
Neurons were isolated from embryonic rat spi-nal ventral tissue and then were primarily cultured in vitro. Morphological feature of neurons was examined by immuno-cytochemical staining with mAb SMI-32. The effect of GDNFon growth of spinal motor neurons was detected by MTT colorimetry.


RESULTS
GDNF could significantly enhance the survival and neurite outgrowth of rat spinal motor neurons cultured in vitro in dose-dependent tendency.


CONCLUSIONS
Varying concentrations of GDNF can enhance the growth of embryonic rat spinal motor neurons to various extent.
Song
Xue-qin
XQ
Department of Neurology,Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
Wu
Shu-yu
SY
Li
Chun-yan
CY
Wang
Li-qin
LQ
Wang
Xiao-juan
XJ
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D051100:Glial Cell Line-Derived Neurotrophic Factor; D007150:Immunohistochemistry; D008297:Male; D009046:Motor Neurons / Q000187:drug effects* / Q000502:physiology; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000187:drug effects* / Q000196:embryology / Q000502:physiology
C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors