title: Applied study on magnetic nanometer beads in preparation of genechip samples.
abstract: A protocol for enrichment and adsorption of karyocyte from whole blood by using magnetic nanometer beads as solid-phase absorbents was presented. The PCR amplification could be accomplished by using the nanobeads with karyocyte as template directly and the PCR products were applied on an oligonucleotide array to do gene typing. The HLA-A PCR amplification system and a small HLA-A oligonucleotide microarray were applied as the platform and an experiment protocol of separating karyocyte from whole blood using the magnetic nanometer beads (Fe2O3) were set up. The experimental conditions were also discussed. It showed that pH level of PBS eluent, Taq enzyme quantity and fragment length of products could influent the amplification results, and the magnetic nano-beads could succeed in sample preparation in microarray to provide a promising way in automatic detection and lab-on-a-chip.
authors.affiliation: Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong Univeristy of Science and Technology, Wuhan 430030.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Huafang
authors.initials: H
authors.lastname: Gao
authors.forename: Xin
authors.initials: X
authors.lastname: Xie
authors.forename: Xuemei
authors.initials: X
authors.lastname: Ma
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000327:Adsorption; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D005838:Genotype; D007962:Leukocytes / Q000166:cytology*; D008280:Magnetics; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods*; D036103:Nanotechnology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D013698:Templates, Genetic