title: CcpA-dependent regulation of Bacillus subtilis glutamate dehydrogenase gene expression.
abstract: The Bacillus subtilis rocG gene, encoding catabolic glutamate dehydrogenase, was found to be subject to direct CcpA-dependent glucose repression. The effect of CcpA required the presence of both the HPr and Crh proteins. The primary CcpA binding site was identified by mutational analysis and DNase I footprinting. In the absence of inducers of the Roc pathway, rocG was still expressed at a low level due to readthrough transcription. CcpA-dependent repression of rocG readthrough transcription proved to contribute to the slow growth rate of B. subtilis cells in glucose-glutamate medium. Increased readthrough expression of rocG was shown to be partially responsible for the growth defect of ccpA strains in glucose-ammonium medium.
authors.affiliation: Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA. bbelit02@granite.tufts.edu
authors.forename: Boris R
authors.initials: BR
authors.lastname: Belitsky
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Abraham L
authors.initials: AL
authors.lastname: Sonenshein
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000494:pharmacology; D005969:Glutamate Dehydrogenase / Q000235:genetics*; D018698:Glutamic Acid / Q000378:metabolism; D008969:Molecular Sequence Data; D009952:Ornithine / Q000494:pharmacology; D011401:Promoter Regions, Genetic
chemical_list: D018698:Glutamic Acid; D009952:Ornithine; D005969:Glutamate Dehydrogenase; D005947:Glucose