title: CD4+ T lymphocytes from Anaplasma marginale major surface protein 2 (MSP2) vaccinees recognize naturally processed epitopes conserved in MSP3.
abstract: Major surface protein 2 (MSP2) and MSP3 of the persistent bovine ehrlichial pathogen Anaplasma marginale are immunodominant proteins that undergo antigenic variation. The recently completed sequence of MSP3 revealed blocks of amino acids in the N and C termini that are conserved with MSP2. This study tested the hypothesis that CD4+ T cells specific for MSP2 recognize naturally processed epitopes conserved in MSP3. At least one epitope in the N terminus and two in the C terminus of MSP2 were also processed from MSP3 and presented to CD4+ T lymphocytes from MSP2-immunized cattle. This T-lymphocyte response to conserved and partially conserved epitopes may contribute to the immunodominance of MSP2 and MSP3.
authors.affiliation: Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USA. wbrown@vetmed.wsu.edu
authors.forename: Wendy C
authors.initials: WC
authors.lastname: Brown
authors.forename: Guy H
authors.initials: GH
authors.lastname: Palmer
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Brayton
authors.forename: Patrick F M
authors.initials: PF
authors.lastname: Meeus
authors.forename: Anthony F
authors.initials: AF
authors.lastname: Barbet
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Kegerreis
authors.forename: Travis C
authors.initials: TC
authors.lastname: McGuire
mesh_terms: D000595:Amino Acid Sequence; D000709:Anaplasma / Q000276:immunology*; D000712:Anaplasmosis / Q000517:prevention & control; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control; D002478:Cells, Cultured; D017124:Conserved Sequence; D018604:Epitope Mapping; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D007114:Immunization; D016056:Immunodominant Epitopes / Q000276:immunology; D008969:Molecular Sequence Data
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D018984:Epitopes, T-Lymphocyte; D016056:Immunodominant Epitopes; C103346:major surface protein 3, Anaplasma marginale; C103348:msp2 protein, Anaplasma marginale