Crystallization and preliminary crystallographic data of SnoaL, a polyketide cyclase in nogalamycin biosynthesis.
Nogalonic acid methyl ester cyclase (SnoaL) catalyzes the last ring-closure step in the biosynthesis of the polyketide antibiotic nogalamycin. Crystals of a complex of SnoaL with the substrate nogalonic acid methyl ester have been obtained using PEG 4000 as precipitant. The crystals are orthorhombic, space group I222, with unit-cell parameters a = 69.1, b = 72.0, c = 65.4 angstroms. They diffract to 1.35 angstroms resolution using synchrotron radiation. A Matthews coefficient of 2.0 angstroms3 Da(-1) suggests one subunit in the asymmetric unit. Diffraction data for an isomorphous uranium derivative were collected and a difference Patterson map showed strong peaks which allowed determination of the position of the uranium ions.
Sultana
Azmiri
A
Molecular Structural Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-171 77 Stockholm, Sweden.
Kallio
Pauli
P
Jansson
Anna
A
Niemi
Jarmo
J
Mäntsälä
Pekka
P
Schneider
Gunter
G
D000595:Amino Acid Sequence; D000903:Antibiotics, Antineoplastic / Q000737:chemistry; D001426:Bacterial Proteins; D018360:Crystallography, X-Ray / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007477:Ions; D007535:Isomerases / Q000737:chemistry*; D008956:Models, Chemical; D008969:Molecular Sequence Data; D009621:Nogalamycin / Q000096:biosynthesis*; D011092:Polyethylene Glycols / Q000737:chemistry; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity; D017356:Synchrotrons; D014501:Uranium / Q000737:chemistry / Q000378:metabolism
D000903:Antibiotics, Antineoplastic; D001426:Bacterial Proteins; D007477:Ions; D011092:Polyethylene Glycols; D014501:Uranium; D007535:Isomerases; C405498:nogalonic acid methyl ester cyclase; D009621:Nogalamycin