title: Quantitative analysis of highly parallel transfection in cell microarrays.
abstract: As more genomes are sequenced, we are facing the challenge of rapidly unraveling the functions of genes. To that end, cell microarrays have recently been described that transfect thousands of nucleic acids in parallel and can be used to analyze the phenotypic consequences of such perturbations. As many parameters can influence the efficacy of transfection in such a format, we describe some important features in manufacturing cell microarrays that may improve reliability and efficiency of both plasmid DNA and siRNA transfection. We have also developed image analysis software that allows automatic detection of cell clusters, quantification of transfection efficiency and levels of expression/extinction of genes. Along with cell microarrays, this bioinformatic tool should expedite functional exploration of the human genome.
authors.affiliation: Service de Génomique Fonctionnelle CEA, 2 Rue Gaston Crémieux CP22, 91057 Evry Cedex, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Baghdoyan
authors.forename: Yoann
authors.initials: Y
authors.lastname: Roupioz
authors.forename: Amandine
authors.initials: A
authors.lastname: Pitaval
authors.forename: David
authors.initials: D
authors.lastname: Castel
authors.forename: Elena
authors.initials: E
authors.lastname: Khomyakova
authors.forename: Alexandre
authors.initials: A
authors.lastname: Papine
authors.forename: Françoise
authors.initials: F
authors.lastname: Soussaline
authors.forename: Xavier
authors.initials: X
authors.lastname: Gidrol
mesh_terms: D001331:Automation; D001704:Biopolymers; D002460:Cell Line; D002477:Cells / Q000378:metabolism*; D019295:Computational Biology / Q000379:methods; D004247:DNA / Q000008:administration & dosage / Q000235:genetics; D005453:Fluorescence; D005780:Gelatin; D020869:Gene Expression Profiling / Q000379:methods; D020868:Gene Silencing; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D015894:Genome, Human; D023281:Genomics / Q000379:methods*; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010641:Phenotype; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D012984:Software; D014162:Transfection / Q000379:methods* / Q000592:standards*
chemical_list: D001704:Biopolymers; D008164:Luminescent Proteins; D034741:RNA, Small Interfering; D049452:Green Fluorescent Proteins; D005780:Gelatin; D004247:DNA