title: Amifostine as differentiating agent in cord blood and bone marrow cultures from children with hematological disorders.
abstract: Amifostine protects normal tissues from the cytotoxicity of chemotherapy and radiotherapy and has an effect a growth factor both in vivo and in vitro. To evaluate its possible hemopoietic promoting activity, the authors studied the effect of WR-2721 on cord blood and bone marrow progenitors from children with hematological disorders and normal controls in semisolid cultures after preincubation with 0.1-1000 microM amifostine. Amifostine enhanced the growth of BFU-E in autoimmune neutropenia but inhibited CFU-GM development. It also exerted an inhibitory effect upon growth of committed progenitors in the groups studied (acute lymphoblastic leukemia, Langerhans cell histiocytosis), including cord blood and marrow of healthy controls.
authors.affiliation: Department of Pediatric Hematology/Oncology, University Hospital of Heraklion, 71110, Heraklion, Crete, Greece.
authors.forename: Vassiliki
authors.initials: V
authors.lastname: Danilatou
authors.forename: Helen
authors.initials: H
authors.lastname: Dimitriou
authors.forename: Eftichia
authors.initials: E
authors.lastname: Stiakaki
authors.forename: Maria
authors.initials: M
authors.lastname: Kalmanti
mesh_terms: D004999:Amifostine / Q000494:pharmacology*; D001854:Bone Marrow Cells; D016022:Case-Control Studies; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002648:Child; D004305:Dose-Response Relationship, Drug; D004341:Drug Evaluation; D005312:Fetal Blood / Q000166:cytology; D006402:Hematologic Diseases / Q000473:pathology*; D006410:Hematopoiesis / Q000187:drug effects*; D006646:Histiocytosis, Langerhans-Cell / Q000473:pathology; D006801:Humans; D023461:Myeloid Progenitor Cells / Q000187:drug effects; D009503:Neutropenia / Q000473:pathology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000473:pathology; D020011:Protective Agents / Q000494:pharmacology
chemical_list: D020011:Protective Agents; D004999:Amifostine