CpG island hypermethylation is maintained in human colorectal cancer cells after RNAi-mediated depletion of DNMT1.
The role of the primary mammalian DNA methyltransferase, DNMT1, in maintaining CpG island methylation in human colon cancer cells has recently been questioned. This controversy has arisen from discrepancies between genetic knockout and RNA interference-mediated knockdown studies. Here, we re-examined the RNA interference-based approach and found that hypermethylation of single-copy genes is maintained in cells transiently and stably depleted of DNMT1.
Ting
Angela H
AH
Program in Cellular and Molecular Medicine, Cancer Biology Division, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, The Johns Hopkins University, Baltimore, Maryland 21231, USA.
Jair
Kam-wing
KW
Suzuki
Hiromu
H
Yen
Ray-Whay Chiu
RW
Baylin
Stephen B
SB
Schuebel
Kornel E
KE
D001483:Base Sequence; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000235:genetics*; D018899:CpG Islands; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation; D020868:Gene Silencing; D016147:Genes, Tumor Suppressor; D045325:HCT116 Cells; D006801:Humans; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D012334:RNA, Neoplasm / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics
D012334:RNA, Neoplasm; D034741:RNA, Small Interfering; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; C000619380:DNMT1 protein, human