[Fusion expression of Neisseria gonorrhoeae outmembrane protein with a mucosal adjuvant].
OBJECTIVE
To express a fusion protein of Neisseria gonorrhoeae with a mucosal adjuvant.


METHODS
The gene coding Loop VI-VIII(PL678) of porin, an out-membrane protein of Neisseria gonorrhoeae, was obtained by PCR. It was inserted into a plasmid fused with subunit B of heat labile enterotoxin. The recombinant was transformed in E. coli. The expression of fusion protein was analysed by ELISA, SDS-PAGE and Western-blot.


RESULTS
Fusion protein with LTB was successfully expressed, and displayed both the ability of binding GM1 and the reactogenicity with polyclonal antibodies against Neisseria gonorrhoeae.


CONCLUSIONS
The expression of fusion protein laid a foundation for the study of the intramolecular vaccine against Neisseria gonorrhoeae.
Pan
Jing
J
College of Medical Laboratory, Third Military Medical University, Chongqing 400038, China.
Mao
Xuhu
X
Zhang
Weijun
W
Lu
Dongshui
D
Luo
Ping
P
Wang
Ning
N
D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis*; D001427:Bacterial Toxins / Q000096:biosynthesis*; D001428:Bacterial Vaccines / Q000276:immunology; D004768:Enterotoxins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D009344:Neisseria gonorrhoeae / Q000737:chemistry* / Q000276:immunology; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D014614:Vaccines, Synthetic / Q000276:immunology
D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; C027538:heat-labile enterotoxin, E coli