Cleavage inhibition of the murine coronavirus spike protein by a furin-like enzyme affects cell-cell but not virus-cell fusion.
Cleavage of the mouse hepatitis coronavirus strain A59 spike protein was blocked in a concentration-dependent manner by a peptide furin inhibitor, indicating that furin or a furin-like enzyme is responsible for this process. While cell-cell fusion was clearly affected by preventing spike protein cleavage, virus-cell fusion was not, indicating that these events have different requirements.
de Haan
Cornelis A M
CA
Virology Division, Department of Infectious Diseases & Immunology, Yalelaan 1, 3584CL Utrecht, The Netherlands. p.rottier@vet.uu.nl
Stadler
Konrad
K
Godeke
Gert-Jan
GJ
Bosch
Berend Jan
BJ
Rottier
Peter J M
PJ
D004705:Endocytosis; D045683:Furin / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008561:Membrane Fusion; D008562:Membrane Glycoproteins / Q000378:metabolism*; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000378:metabolism*
D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; D045683:Furin