title: Application of liquid chromatography-tandem mass spectrometry for the determination of opioidmimetics in the brain dialysates from rats treated with opioidmimetics intraperitoneally.
abstract: We have determined three opioidmimetics (compounds I-III) in the rat brain dialysates after intraperitoneal (i.p.) administration of compounds I-III using a liquid chromatography/mass spectrometry with tandem mass spectrometry (LC-MS/MS). The dialysate samples with methanol were directly analyzed by online column-switching liquid chromatography. Using multiple reaction monitoring (MRM, product ions m/z 421 of m/z 657 for compound I, m/z 421 of m/z 643 for compound II, and m/z 407 of m/z 629 for compound III) on LC-MS/MS with electrospray ionization (ESI), opioidmimetics in rat brain dialysates were determined. Calibration curves of the method showed a good linearity in the range of 10-100 ng/ml for each compound. The limit of determination was estimated to be ca. 1 ng/ml for compounds II and III, and ca. 5 ng/ml for compound I, respectively. The precision of analysis showed coefficients of variation ranging from 4.7 to 10.4% at compound III concentration (10-100 ng/ml) in Ringer's solution. As a result, the procedure proved to be very suitable for routine analysis. The method was applied to the analysis of three opioidmimetics in the brain dialysate samples from rats treated with these compounds.
authors.affiliation: Department of Analytical Toxicology, Faculty of Pharmaceutical Sciences, Kobe Gakuin University, Ikawadani-cho, Nishi-ku, Kobe 651-2180, Japan. igarashi@pharm.kobegakuin.ac.jp
authors.forename: Kazuo
authors.initials: K
authors.lastname: Igarashi
authors.forename: Yuki
authors.initials: Y
authors.lastname: Murabayashi
authors.forename: Koichiro
authors.initials: K
authors.lastname: Hotta
authors.forename: Youji
authors.initials: Y
authors.lastname: Kitamura
authors.forename: Fumiyo
authors.initials: F
authors.lastname: Kasuya
authors.forename: Kimitaka
authors.initials: K
authors.lastname: Shiotani
authors.forename: Li
authors.initials: L
authors.lastname: Tingyou
authors.forename: Anna
authors.initials: A
authors.lastname: Miyazaki
authors.forename: Yuko
authors.initials: Y
authors.lastname: Tsuda
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Okada
authors.forename: Shoji
authors.initials: S
authors.lastname: Fukushima
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D007274:Injections, Intraperitoneal; D008297:Male; D017551:Microdialysis; D018716:Molecular Mimicry; D009294:Narcotics / Q000008:administration & dosage / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D009294:Narcotics