Modified-vaccinia-virus-Ankara (MVA) priming and fowlpox-virus booster elicit a stronger CD8+ T-cell response in mice against an HIV-1 epitope than does a DNA/poxvirus prime-booster approach.
A prime-boost strategy combining FWPV (fowlpox virus) and the MVA (modified vaccinia virus Ankara), both expressing HIV-1 multi-V3 epitope polypeptides, was compared with a DNA-based Semliki Forest virus replicon/poxvirus approach for the induction of a CD8(+) T-cell response. Priming mice with recombinant MVA and boosting with recombinant FWPV, and not in the reverse order, increased the number of specific interferon-gamma-secreting cells in relation to the homologous combinations. Moreover, the improvement of the CD8(+) T-cell response with this combination was remarkably higher than that obtained by priming with a DNA vector containing a Semliki Forest virus replicon expressing the multi-epitope polypeptide and boosting either with recombinant MVA or FWPV. These results open a new and attractive alternative for vaccine preparation against HIV-1 using different immunogens.
Vázquez-Blomquist
Dania
D
Departamento de SIDA, División de Vacunas, Centro de Ingeniería Genética y Biotecnología, Apdo 6162, Ciudad de La Habana 10600, Cuba.
Quintana
Diógenes
D
Duarte
Carlos A
CA
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D045744:Cell Line, Tumor; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005260:Female; D005587:Fowlpox virus / Q000235:genetics / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D007117:Immunization, Secondary; D007371:Interferon-gamma / Q000378:metabolism; D034801:Mastocytoma / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D011995:Recombination, Genetic; D013154:Spleen / Q000166:cytology; D014611:Vaccination; D019444:Vaccines, DNA; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology*
D000939:Epitopes; D019444:Vaccines, DNA; D007371:Interferon-gamma