title: DNA microarray screening of differential gene expression in bone marrow samples from AML, non-AML patients and AML cell lines.
abstract: This study used cDNA microarray technology to compare gene expression profiles in acute myeloblastic leukaemia (AML) with cDNA dot-blot and real time PCR analysis of cDNA transcripts to confirm array data. Patient AML marrow samples and AML cell lines were compared with normal/non-AML samples. Screening revealed five particular genes to be significantly differentially expressed across the sample groups. The migration-inhibitory factor-related-proteins 8 and 14 (MRP-8 and MRP-14) genes, the products of which inhibit cell migration and differentiation were the most highly expressed in non-malignant cells. The high-mobility-group-protein gene (HMG-1) was up regulated in leukaemic samples and cell lines, which may be associated with aggressive disease. Also upregulated in malignant samples were genes encoding c-myc and glutathione-S-transferase pi (GSTP), the latter implicated in chemotherapy resistance. Faulty expression of such genes may contribute to the pathogenesis of AML and resistance to treatment.
authors.affiliation: Centre for Research in Biomedicine, University of the West of England, Bristol BS16 1QY, UK.
authors.forename: Emma Louise
authors.initials: EL
authors.lastname: Court
authors.forename: M Ann
authors.initials: MA
authors.lastname: Smith
authors.forename: Neil David
authors.initials: ND
authors.lastname: Avent
authors.forename: John T
authors.initials: JT
authors.lastname: Hancock
authors.forename: Lyn M
authors.initials: LM
authors.lastname: Morgan
authors.forename: Atherton G
authors.initials: AG
authors.lastname: Gray
authors.forename: J Graham
authors.initials: JG
authors.lastname: Smith
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D001853:Bone Marrow / Q000473:pathology; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000235:genetics; D024243:HMGB1 Protein / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism / Q000473:pathology*; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis / Q000235:genetics; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis / Q000235:genetics; D015854:Up-Regulation
chemical_list: D024243:HMGB1 Protein; D008263:Macrophage Migration-Inhibitory Factors; D016271:Proto-Oncogene Proteins c-myc; D005982:Glutathione Transferase