title: Improved sensitivity for insulin in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry by premixing alpha-cyano-4-hydroxycinnamic acid matrix with transferrin.
abstract: This report describes an enhancement of the signal intensities of proteins and peptides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). When alpha-cyano-4-hydroxycinnamic acid (CHCA) premixed with human transferrin (Tf) was used as a matrix, the signal intensity of insulin was amplified to more than ten times that of the respective control in CHCA without Tf. The detection limit of insulin was 0.39 fmol on-probe in the presence of Tf, while it was 6.3 fmol in the absence of Tf. The signal intensity of insulin was also enhanced when the CHCA matrix was premixed with proteins other than Tf (80 kDa), such as horse ferritin (20 kDa), bovine serum albumin (BSA, 66 kDa), or human immunoglobulin G (150 kDa). The optimum spectrum of insulin was obtained when the added amount of protein was in the range 0.26-0.62 pmol, regardless of the molecular weight of the added protein. Tf and BSA outperformed the other tested proteins, as determined by improvements in the resulting spectra. When the mass spectra of several peptides and proteins were recorded in the presence of Tf or BSA, the signal intensities of large peptides such as glucagon were enhanced, though those of smaller peptides were not enhanced. In addition, the signal enhancement achieved with Tf and BSA was more pronounced for the proteins, including cytochrome C, than for the large peptides. This enhancement effect could be applied to improve the sensitivity of MALDI-TOFMS to large peptides and proteins.
authors.affiliation: Division of Biological Chemistry and Biologicals, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan. kobayash@nihs.go.jp
authors.forename: Tetsu
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawai
authors.forename: Takuo
authors.initials: T
authors.lastname: Suzuki
authors.forename: Toru
authors.initials: T
authors.lastname: Kawanishi
authors.forename: Takao
authors.initials: T
authors.lastname: Hayakawa
mesh_terms: D000818:Animals; D002417:Cattle; D003373:Coumaric Acids; D005293:Ferritins; D006736:Horses; D006801:Humans; D007074:Immunoglobulin G; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D007328:Insulin / Q000032:analysis*; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods* / Q000706:statistics & numerical data; D014168:Transferrin
chemical_list: D003373:Coumaric Acids; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D007328:Insulin; D010455:Peptides; D011506:Proteins; D014168:Transferrin; D012710:Serum Albumin, Bovine; C007175:alpha-cyano-4-hydroxycinnamate; D005293:Ferritins