Prenatal exposure to ethanol causes partial diabetes insipidus in adult rats.
Chronic consumption of ethanol in adult rats and humans leads to reduced AVP-producing neurons, and prenatal ethanol (PE) exposure has been reported to cause changes in the morphology of AVP-producing cells in the suprachiasmatic nucleus of young rats. The present studies further characterize the effects of PE exposure on AVP in the young adult rat, its hypothalamic synthesis, pituitary storage, and osmotically stimulated release. Pregnant rats were fed a liquid diet with 35% of the calories from ethanol or a control liquid diet for days 7-22 of pregnancy. Water consumption and urine excretion rate were measured in the offspring at 60-68 days of age. Subsequently, the offspring were infused with 5% NaCl at 0.05 ml.kg(-1).min(-1) with plasma samples taken before and at three 40-min intervals during infusion for measurement of AVP and osmolality. Urine output and water intake were approximately 20% greater in PE-exposed rats than in rats with no PE exposure, and female rats had a greater water intake than males. The relationship between plasma osmolality and AVP in PE-exposed rats was parallel to, but shifted to the right of, the control rats, indicating an increase in osmotic threshold for AVP release. Pituitary AVP was reduced by 13% and hypothalamic AVP mRNA content was reduced by 35% in PE-exposed rats. Our data suggest that PE exposure can cause a permanent condition of a mild partial central diabetes insipidus.
Knee
Daniel S
DS
Department of Pediatrics, Tripler Army Medical Center, Hawaii 96859-5000, USA.
Sato
Aileen K
AK
Uyehara
Catherine F T
CF
Claybaugh
John R
JR
D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism; D001794:Blood Pressure; D002492:Central Nervous System Depressants / Q000494:pharmacology*; D003919:Diabetes Insipidus / Q000139:chemically induced* / Q000503:physiopathology*; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D063647:Fetal Alcohol Spectrum Disorders / Q000503:physiopathology; D006339:Heart Rate; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D010902:Pituitary Gland / Q000378:metabolism; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D014882:Water-Electrolyte Balance / Q000187:drug effects
D002492:Central Nervous System Depressants; D001127:Arginine Vasopressin; D000431:Ethanol