title: The brain-specific double-stranded RNA-binding protein Staufen2: nucleolar accumulation and isoform-specific exportin-5-dependent export.
abstract: The mammalian double-stranded RNA-binding proteins Staufen (Stau1 and Stau2) are involved in RNA localization in polarized neurons. In contrast to the more ubiquitously expressed Stau1, Stau2 is mainly expressed in the nervous system. In Drosophila, the third double-stranded RNA-binding domain (RBD3) of Staufen is essential for RNA interaction. When conserved amino acids within the RBD3 of Stau2 were mutated to render Stau2 defective for RNA binding, the mutant Stau2 proteins accumulate predominantly in the nucleolus. This is in contrast to wild type Stau2 that mostly localizes in the cytosol. The nuclear import is dependent on a nuclear localization signal in close proximity to the RBD3. The nuclear export of Stau2 is not dependent on CRM1 but rather on Exportin-5. We show that Exportin-5 interacts with the RBD3 of wild type Stau2 in an RNA-dependent manner in vitro but not with mutant Stau2. When Exportin-5 is down-regulated by RNA interference, only the largest isoform of Stau2 (Stau2(62)) preferentially accumulates in the nucleolus. It is tempting to speculate that Stau2(62) binds RNA in the nucleus and assembles into ribonucleoparticles, which are then exported via the Exportin-5 pathway to their final destination.
authors.affiliation: Max-Planck-Institute for Developmental Biology, D-72076 Tübingen, Germany.
authors.forename: Paolo
authors.initials: P
authors.lastname: Macchi
authors.forename: Amy M
authors.initials: AM
authors.lastname: Brownawell
authors.forename: Barbara
authors.initials: B
authors.lastname: Grunewald
authors.forename: Luc
authors.initials: L
authors.lastname: DesGroseillers
authors.forename: Ian G
authors.initials: IG
authors.lastname: Macara
authors.forename: Michael A
authors.initials: MA
authors.lastname: Kiebler
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D001921:Brain / Q000378:metabolism*; D002460:Cell Line; D002466:Cell Nucleolus / Q000378:metabolism*; D006224:Cricetinae; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D028884:Karyopherins / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D019913:Nuclear Localization Signals; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020931:ran GTP-Binding Protein / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D028884:Karyopherins; D019913:Nuclear Localization Signals; D020033:Protein Isoforms; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; C446511:XPO5 protein, human; C069927:stau protein, Drosophila; D020931:ran GTP-Binding Protein