title: Effect of hydrophilic coating on microorganism colonization in silicone tubing.
abstract: BACKGROUND
  Shunt infections are one of the major causes of mortality and morbidity of patients with hydrocephalus. The aim of this research is to compare the bacterial colonization characteristics of a regular silicone elastomer shunt material coated with polyvinylpyrrolidone and dimethylpolysiloxane (silicone).
  
  
  METHODS
  Regular coated shunt materials were compared by in-vivo and in-vitro methods. In the in-vitro experiment, silicone and coated material immersed and not immersed in vancomycin solution was treated with a certain concentration of Staphylococcus epidermidis. In the in-vivo study, silicone and coated material specimens were treated with Staphylococcus epidermidis and they were stereotactically placed in the lateral ventricles of the rats. One week after the inoculation, shunt pieces were removed and the colonies were counted by using a scanning electron microscope.
  
  
  RESULTS
  There was a statistically significant difference of colonization in the in-vitro groups in coated material vs. silicone, coated material vs. vancomycin treated silicone, vancomycin treated coated material vs. silicone, vancomycin treated coated material vs. vancomycin treated silicone. There was no statistically significant difference for colonization in in-vitro groups of coated material and vancomycin treated coated material. With in-vivo experiments we can say that, coated material catheters are superior than the silicone catheters in respect to colonization but after the bacterial colonization has occurred, the amount of colonization did not differ.
  
  
  CONCLUSIONS
  Coated material catheters are superior to silicone catheters and they prevent bacterial colonization in some respect.
authors.affiliation: Department of Neurosurgery, Karaelmas University FOM, Zonguldak, Turkey. cagavif@yahoo.com
authors.forename: F
authors.initials: F
authors.lastname: Cağavi
authors.forename: N
authors.initials: N
authors.lastname: Akalan
authors.forename: H
authors.initials: H
authors.lastname: Celik
authors.forename: D
authors.initials: D
authors.lastname: Gür
authors.forename: B
authors.initials: B
authors.lastname: Güçiz
mesh_terms: D000818:Animals; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D002557:Cerebrospinal Fluid Shunts / Q000295:instrumentation*; D020099:Coated Materials, Biocompatible; D015169:Colony Count, Microbial; D006801:Humans; D006849:Hydrocephalus / Q000601:surgery*; D066298:In Vitro Techniques; D008297:Male; D008855:Microscopy, Electron, Scanning; D011205:Povidone; D051381:Rats; D017207:Rats, Sprague-Dawley; D012826:Silicone Elastomers; D012828:Silicones; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000254:growth & development*; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D020099:Coated Materials, Biocompatible; D012826:Silicone Elastomers; D012828:Silicones; D014640:Vancomycin; D011205:Povidone