[Effects of altered peptide ligands (APLs) of proteolipid(136-150) on T-cell clone 4B.14a].
OBJECTIVE
By investigating the effects of APLs of PLP(136-150) on T cell clone 4B.14a in-vitro and in-vivo, to determine the feasibility of APLs to prevent relapsing multiple sclerosis (MS) in human.


METHODS
To mimic the clinic course of relapsing MS, SJL/J female mice were first irradiated at 450R and intravenously infused with 1x10(7) resting 4B.14a T cells per mouse. Then mice were immunized with 50 microg/mouse APL for inducing passive experiment allergic encephalomyelitis (EAE). The proliferation and cytokine production of 4B.14a T cells in response to APLs were also examined.


RESULTS
Except 139A, 143A, 144A, 145A and 148A, other APLs triggered T cells to induce passive EAE; 4B.14a T cells well responded to the most APLs, weakly responded to 137A, 144A and 148A, but the response was suppressed by 139A.


CONCLUSIONS
The effects of some APLs on 4B.14a T cells are different in-vitro and in-vivo. It may be feasible to select some APLs to prevent relapsing MS in human.
Geng
Tong-Chao
TC
Department of Neurology, China-Japan Friendship Hospital, Beijing 100029, China.
D000818:Animals; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology; D006801:Humans; D008024:Ligands; D009103:Multiple Sclerosis; D010455:Peptides / Q000276:immunology; D011510:Proteolipids; D013601:T-Lymphocytes / Q000276:immunology
D008024:Ligands; D010455:Peptides; D011510:Proteolipids