Biomaterial surface-dependent neutrophil mobility.
Compromised neutrophil function in the presence of an implanted biomaterial may represent an important mechanism that allows for the development of implant-associated infections. Here, human neutrophil mobility has been investigated on a polyurethane (ChronoFlex AR), a hydrophobic surface consisting of an octadecyltrichlorosilane (OTS) self-assembled monolayer, and a glass reference material. Neutrophil mobility was quantified, based on cell movement speed and persistence time obtained from time-lapse optical microscopy, while neutrophil cytoskeletal structures and morphology were visualized using confocal microscopy and atomic force microscopy. Our results show that material surface properties affect neutrophil-surface interactions, as reflected by morphological changes, and the mobility of neutrophils stimulated by N-formylmethionyl-leucyl-phenylalanine (fMLP). In the absence of adsorbed plasma proteins, the mobility of stimulated neutrophils increased with increasing material hydrophobicity from glass, to polyurethane, to OTS. The opposite trend was observed in the presence of adsorbed plasma proteins, such that neutrophil mobility increased with decreasing material hydrophobicity. Analysis of the results showed that the mobility of fMLP-stimulated neutrophils cells was inversely related to the extent of cell spreading on the materials.
Zhou
Yue
Y
Department of Biomedical Engineering, Case Western Reserve University, Wickenden Building, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
Doerschuk
Claire M
CM
Anderson
James M
JM
Marchant
Roger E
RE
D001672:Biocompatible Materials; D002465:Cell Movement / Q000502:physiology*; D005898:Glass; D006801:Humans; D018625:Microscopy, Atomic Force; D009504:Neutrophils / Q000166:cytology / Q000502:physiology*; D011140:Polyurethanes; D012821:Silanes; D013997:Time Factors
D001672:Biocompatible Materials; D011140:Polyurethanes; D012821:Silanes; C061189:octadecyltrichlorosilane