Somatic embryogenesis and plant regeneration from immature zygotic embryos of Hinoki cypress (Chamaecyparis obtusa Sieb. et Zucc.).
We established a plant regeneration system for Hinoki cypress (Chamaecyparis obtusa) via somatic embryogenesis. Embryogenic tissues were successfully induced on three kinds of Smith media from megagametophyte explants containing pre-cotyledonary embryos of C. obtusa plus-trees. Factors affecting somatic embryo maturation were examined. The concentration of polyethylene glycol 4000 in the medium was a critical factor for embryo maturation and its effective concentration was 150 g/l. The addition of 30 g/l maltose to the medium had a positive effect on embryo maturation, but sucrose was ineffective. The mature somatic embryos germinated at a germination frequency of approximately 60%, and the presence of activated charcoal was effective in stimulating plantlet growth. The plantlets acclimatized successfully in a greenhouse. To our knowledge, this is first report describing details of a plant regeneration method for C. obtusa via somatic embryogenesis.
Taniguchi
T
T
Forest Tree Breeding Center, 3809-1 Ishi, Juo, 319-1301, Taga, Ibaraki, Japan. toru.t@affrc.go.jp
Kurita
M
M
Itahana
N
N
Kondo
T
T
D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D029780:Chamaecyparis / Q000166:cytology / Q000196:embryology* / Q000502:physiology*; D002606:Charcoal / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005818:Genetic Engineering / Q000379:methods; D018525:Germination / Q000187:drug effects / Q000502:physiology; D008320:Maltose / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D012639:Seeds / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D013395:Sucrose / Q000494:pharmacology
D003470:Culture Media; D002606:Charcoal; D011092:Polyethylene Glycols; D013395:Sucrose; D008320:Maltose