[Characterization of Mycobacterium tuberculosis polypeptide antigen capable of stimulating the proliferation of human gammadeltaT cells].
OBJECTIVE
To analyse biological properties of Mycobacterium tuberculosis polypeptide antigen(Mtb-Ag) that could stimulate the proliferation of human gammadeltaT cells.


METHODS
Mtb secretory proteins and Mtb-Ag treated via dialysis or with pronase were used to stimulate human PBMCs. After being cultured for 10 days, the phenotype of responsive cells was analysed by flow cytometry.


RESULTS
The proliferation stimulating activity to human gammadeltaT cells of dialysed Mtb-Ag did not decrease remarkably, whereas that of the pronase-treated Mtb-Ag reduced significantly. Moreover, the proliferative stimulating activity to gammadeltaT cells of Mtb-Ag was notably higher than Mtb secretory protein.


CONCLUSIONS
The component in Mtb-Ag that can stimulate the proliferation of human gammadeltaT cells is a non-secretory and protase-sensitive polypeptides with M(r) over 10 000.
Chen
Yong
Y
Department of Immunology, Bengbu Medical College, Bengbu 233003,China.
Lu
He-Zuo
HZ
Hu
Jian-Guo
JG
Li
Bai-Qing
BQ
D000942:Antigens, Bacterial / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation; D009169:Mycobacterium tuberculosis / Q000235:genetics; D010455:Peptides; D014376:Tuberculosis / Q000382:microbiology
D000942:Antigens, Bacterial; D010455:Peptides