title: The fate of mitochondria in Ibex-hirus reconstructed early embryos.
abstract: Inter-species nuclear transfer could be used to preserve North Goat (Capra ibex), an endangered species. We established the culture conditions for ibex-hirus reconstructed embryos and optimized the method for DNA extractions of a single cell and early cloned embryo. By using mitochondria-specific probes of ibex and hirus respectively we found that mitochondria of donor cells can co-exist with recipients in 1-cell and 2-cell stages of the reconstructed embryos but not in the following developmental stages.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, the Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
authors.forename: Shu-Zhen
authors.initials: SZ
authors.lastname: Liu
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Zhang
authors.forename: Man-Xi
authors.initials: MX
authors.lastname: Jiang
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Sun
authors.forename: Da-Yuan
authors.initials: DY
authors.lastname: Chen
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000196:embryology; D019976:Cloning, Organism / Q000379:methods*; D004272:DNA, Mitochondrial / Q000235:genetics; D004622:Embryo, Mammalian / Q000502:physiology* / Q000601:surgery*; D005314:Embryonic and Fetal Development / Q000235:genetics; D008928:Mitochondria / Q000502:physiology*; D053652:Nuclear Transfer Techniques; D012418:Ruminants / Q000196:embryology* / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial