Hemodilution with lactated Ringer's solution causes hypocoagulability in rabbits.
Hemodilution (HD) has been associated with hypercoagulability. It was hypothesized that HD with lactated Ringer's solution (LR) would result in hypercoagulability in rabbits. Sedated rabbits (n = 12) underwent HD with LR (40% estimated blood volume replaced with five volumes of LR) via ear vessels. Key procoagulants and anticoagulant activities were assessed prior to and 3 h after HD. Hemostatic function was assessed with the activated coagulation time and platelet-inhibited thrombelastography. Circulating tissue factor activity was much more diluted (-67.2% from baseline) than tissue factor pathway inhibitor (-45.2%) or antithrombin (-9.5%) activities after HD. HD significantly decreased factor VIII complex activity (-31.5%) more than protein C activity (-5.9%), and factor X activity (-29.2%) was more diluted than antithrombin activity. The activated coagulation time and thrombelastography demonstrated a significant decrease in hemostatic function after HD. Hemodilution with LR caused hypocoagulability in the rabbit. A greater decrease in circulating procoagulant activity than anticoagulant activity appears to be the mechanism underlying HD-mediated decreases in hemostasis.
Nielsen
Vance G
VG
Department of Anesthesiology, Division of Cardiothoracic Anesthesia, The University of Alabama at Birmingham, Alabama 35249-6810, USA. vance.nielsen@ccc.uab.edu
D000818:Animals; D000991:Antithrombins / Q000032:analysis; D001777:Blood Coagulation; D001778:Blood Coagulation Disorders / Q000097:blood* / Q000209:etiology; D005169:Factor VIII / Q000032:analysis; D005170:Factor X / Q000032:analysis; D006438:Hemodilution / Q000009:adverse effects*; D007552:Isotonic Solutions / Q000008:administration & dosage*; D008074:Lipoproteins / Q000097:blood; D011817:Rabbits; D000077325:Ringer's Lactate; D013916:Thrombelastography; D013925:Thromboplastin / Q000032:analysis
D000991:Antithrombins; D007552:Isotonic Solutions; D008074:Lipoproteins; D000077325:Ringer's Lactate; C051928:lipoprotein-associated coagulation inhibitor; D005169:Factor VIII; D005170:Factor X; D013925:Thromboplastin