Effect of chlorophyllin on normothermic storage of human periodontal ligament cells.
The purpose of the present study was to evaluate whether chlorophyllin could serve as an effective constituent of a storage medium to enhance the human periodontal ligament (PDL) cell viability. Freshly isolated PDL cells from premolars extracted from healthy people were stored at 37 degrees C for 6 h in various solutions: F-medium and Hank's balanced salt solution (HBSS), supplemented with chlorophyllin. From MTT viability assays, the highest cell viability was found in the PDL cells stored in HBSS supplemented with 500 nM chlorophyllin, and the chlorophyllin-treated cells showed a dose-dependent response to concentration. Additionally, the results from flow cytometry showed that 77 to 80% of the PDL cells were in the G0/G1 phases of the cell cycle, which suggested that most were in a stable stage. These result showed that HBSS, supplemented with chlorophyllin, may be a useful solution for preserving the viability of PDL cells.
Chung
Won-Gyun
WG
Department of Dental Hygiene, Yonsei University, Wonju College of Medicine, Wonju-City, Kangwon-Do, Korea.
Lee
Eun Ju
EJ
Lee
Seung-Jong
SJ
Lee
Seung-Ae
SA
Kim
Jin
J
D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002735:Chlorophyllides / Q000494:pharmacology*; D004396:Coloring Agents; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D016193:G1 Phase; D006801:Humans; D007552:Isotonic Solutions / Q000494:pharmacology; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D016192:Resting Phase, Cell Cycle; D013696:Temperature; D013778:Tetrazolium Salts; D013844:Thiazoles; D014021:Tissue Preservation
D000975:Antioxidants; D002735:Chlorophyllides; D004396:Coloring Agents; D003470:Culture Media; C072670:Hanks Balanced Salt Solution; D007552:Isotonic Solutions; D013778:Tetrazolium Salts; D013844:Thiazoles; C007020:chlorophyllin; C022616:thiazolyl blue