Production of monoclonal antibody against ginkgolic acids in Ginkgo biloba Linn.
A competitive enzyme-linked immunosorbent assay (ELISA) for ginkgolic acids (GAs) was developed using monoclonal antibody (MAb) 9F raised against 6-(13-formylheptyl) salicylic acid covalently coupled to bovine serum albumin (BSA). ELISA, at an effective measuring range of 300 ng/ml-1 microgram/ml of GA15:1, was successful in detecting GAs content in ginkgo leaves and standardized extracts due to the lack of cross-reactivity against various related compounds. The sensitive and simple immunoassay developed in this study was validated to be specific for the quantitative determination of total GAs content in ginkgo crude drugs with no interference from the sample matrix. The analytical recovery of spiked GA15:1 was 103% in a concentration range between 10 and 40 mg/g dry weight of ginkgo leaves.
Loungratana
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Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
Tanaka
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H
Shoyama
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Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D020441:Ginkgo biloba; D008297:Male; D008516:Medicine, Chinese Traditional / Q000592:standards; D051379:Mice; D008807:Mice, Inbred BALB C; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves; D012459:Salicylates / Q000276:immunology*; D000096983:Ginkgo Extract
D000911:Antibodies, Monoclonal; D010936:Plant Extracts; D012459:Salicylates; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract; C112485:ginkgolic acid