title: Polyamines reduces lipid peroxidation induced by different pro-oxidant agents.
abstract: Polyamines, among other functions, are considered to act as a free radical scavenger and antioxidant. The quinolinic acid (QA), sodium nitroprusside (SNP) and iron (Fe+2) stimulate production of free radicals and lipid peroxidation. In the present study, we investigated the free radical and/or aldehyde scavenger effects of polyamines spermine and spermidine on thiobarbituric acid reactive species (TBARS) production induced by QA, SNP, Fe+2/EDTA system and free Fe2+ in rat brain. Spermine and spermidine inhibited QA-induced TBARS production; however spermine was a better antioxidant than spermidine. Spermine also inhibited SNP-, Fe+2/EDTA- and free Fe2+-induced TBARS production, but had a modest effect. Spermidine, in turn, also discretely inhibited SNP-, Fe+2/EDTA- and free Fe2+-induced TBARS production. In the presence of MK-801, QA-induced TBARS production was considerably more inhibited by polyamines. In addition, arcaine does not affect the reducer effect of polyamines. The present findings suggest that the observed effects of polyamines are not related to the activation of NMDA receptor but with their antioxidant and free radical scavenger properties.
authors.affiliation: Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus UFSM, Santa Maria, RS 97105-900, Brazil.
authors.forename: Nádia Aléssio Velloso
authors.initials: NA
authors.lastname: Bellé
authors.forename: Gerusa Duarte
authors.initials: GD
authors.lastname: Dalmolin
authors.forename: Graciela
authors.initials: G
authors.lastname: Fonini
authors.forename: Maribel Antonello
authors.initials: MA
authors.lastname: Rubin
authors.forename: João Batista Teixeira
authors.initials: JB
authors.lastname: Rocha
mesh_terms: D000818:Animals; D000975:Antioxidants; D001645:Biguanides / Q000494:pharmacology; D015317:Biogenic Polyamines / Q000494:pharmacology*; D016291:Dizocilpine Maleate / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D007501:Iron / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects*; D009599:Nitroprusside / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology*; D017378:Quinolinic Acid / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001645:Biguanides; D015317:Biogenic Polyamines; D016877:Oxidants; D017392:Thiobarbituric Acid Reactive Substances; D009599:Nitroprusside; D013096:Spermine; C006624:arcaine; D016291:Dizocilpine Maleate; D004492:Edetic Acid; D007501:Iron; D017378:Quinolinic Acid; D013095:Spermidine