Luminescent pH sensing and DNA binding properties of a novel ruthenium(II) complex.
A new Ru(II) complex, [Ru(bpy)(2)(dhipH3)](ClO4)(2) (in which bpy=2,2'-bipyridine, dhipH(3)=3,4-dihydroxy-imidado[4,5-f][1,10]-phenanthroline), was synthesized and characterized, and the pH effect on the emission spectra of the complex was studied. The interaction of the complex with calf thymus DNA was investigated by UV-visible and emission spectroscopy, and viscosity measurements. The results suggest that the complex acted as a sensitive luminescent pH sensor and a strong ct-DNA intercalator with an intrinsic binding constant of (4.0+/-0.7) x 10(5) M(-1) in buffered 50 mM NaCl.
Bai
Guang-Yao
GY
Department of Chemistry, Beijing Normal University, Xinjiecouwai Street 19, Beijing 100875, China.
Wang
Ke-Zhi
KZ
Duan
Zhi-Ming
ZM
Gao
Li-Hua
LH
D004247:DNA / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007364:Intercalating Agents / Q000138:chemical synthesis* / Q000737:chemistry*; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D007364:Intercalating Agents; D009942:Organometallic Compounds; D012428:Ruthenium; D004247:DNA