title: Determining virological, serological and immunological parameters of EBV infection in the development of PTLD.
abstract: Post-transplant lymphoproliferative disease (PTLD) in Epstein-Barr virus (EBV) seronegative solid organ transplant recipients remains a significant problem, particularly in the first year post-transplant. Immune monitoring of a cohort of high-risk patients indicated that four EBV seronegative transplant recipients developed early-onset PTLD prior to evidence of an EBV humoral response. EBV status has been classically defined serologically, however these patients demonstrated multiple parameters of EBV infection, including the generation of EBV-specific CTL, outgrowth of spontaneous lymphoblastoid cell lines, and elevated EBV DNA levels, despite the absence of a classic EBV antibody response. As EBV serology is influenced by both immunosuppression and cytomegalovirus immunoglobulin treatment, both the EBV-specific CTL response and elevated EBV levels are more reliable indicators of EBV infection post-transplant.
authors.affiliation: Division of Immunology and Infectious Diseases and the Cooperative Research Centre for Vaccine Technology, The Queensland Institute of Medical Research and University of Queensland Joint Oncology Program, Brisbane, 4029 Australia. joanne.Da@qimr.edu.au
authors.forename: Joanne E
authors.initials: JE
authors.lastname: Davis
authors.forename: Martina A
authors.initials: MA
authors.lastname: Sherritt
authors.forename: Mandvi
authors.initials: M
authors.lastname: Bharadwaj
authors.forename: Leanne E
authors.initials: LE
authors.lastname: Morrison
authors.forename: Suzanne L
authors.initials: SL
authors.lastname: Elliott
authors.forename: Laurie M
authors.initials: LM
authors.lastname: Kear
authors.forename: Joanne
authors.initials: J
authors.lastname: Maddicks-Law
authors.forename: Tom
authors.initials: T
authors.lastname: Kotsimbos
authors.forename: Devinder
authors.initials: D
authors.lastname: Gill
authors.forename: Monique
authors.initials: M
authors.lastname: Malouf
authors.forename: Michael C
authors.initials: MC
authors.lastname: Falk
authors.forename: Rajiv
authors.initials: R
authors.lastname: Khanna
authors.forename: Denis J
authors.initials: DJ
authors.lastname: Moss
mesh_terms: D000293:Adolescent; D000328:Adult; D004279:DNA, Viral / Q000097:blood*; D020031:Epstein-Barr Virus Infections / Q000097:blood / Q000175:diagnosis / Q000276:immunology* / Q000628:therapy / Q000821:virology; D005260:Female; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007116:Immunization, Passive; D007165:Immunosuppression Therapy; D016031:Liver Transplantation; D016040:Lung Transplantation; D008232:Lymphoproliferative Disorders / Q000175:diagnosis / Q000209:etiology / Q000276:immunology* / Q000821:virology; D008297:Male; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000821:virology
chemical_list: D004279:DNA, Viral