Identification of an oxidative stress-sensitive protein from Campylobacter jejuni, homologous to rubredoxin oxidoreductase/rubrerythrin.
An oxidative stress-sensitive protein was found in the microaerophile Campylobacter jejuni. A novel 27-kDa protein was found to decrease concomitantly with a decrease in viability from either exogenous H(2)O(2) stress or endogenous oxidative stresses in aerobic conditions. Sequence analyses revealed that the 27-kDa protein was identical to Cj0012c in C. jejuni NCTC11168 and its deduced 215 amino acid sequence has similarity to two non-heme iron proteins found in other bacteria, rubredoxin oxidoreductase (Rbo) and rubrerythrin (Rbr). Thus, we designated the protein as Rrc (Rbo/Rbr-like protein of C. jejuni). In H(2)O(2)-treated cells, Western blot analysis showed some bands smaller than Rrc, and RT-PCR showed similar expression of Rrc mRNA to the control without treatment, suggesting that the sensitive response of Rrc to oxidative stress is due to degradation of the protein.
Yamasaki
Manabu
M
Division of Biomedical Food Research, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
Igimi
Shizunobu
S
Katayama
Yoko
Y
Yamamoto
Shigeki
S
Amano
Fumio
F
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D016123:Campylobacter jejuni / Q000187:drug effects / Q000378:metabolism*; D005288:Ferredoxins / Q000737:chemistry; D006422:Hemerythrin; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000378:metabolism; D012416:Rubredoxins; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D005288:Ferredoxins; D006422:Hemerythrin; D012333:RNA, Messenger; D012416:Rubredoxins; C055857:rubrerythrins; D006861:Hydrogen Peroxide; C023091:rubredoxin-NAD+ reductase; D009247:NADH, NADPH Oxidoreductases