title: Artificially ambiguous genetic code confers growth yield advantage.
abstract: A primitive genetic code is thought to have encoded statistical, ambiguous proteins in which more than one amino acid was inserted at a given codon. The relative vitality of organisms bearing ambiguous proteins and the kinds of pressures that forced development of the highly specific modern genetic code are unknown. Previous work demonstrated that, in the absence of selective pressure, enforced ambiguity in cells leads to death or to sequence reversion to eliminate the ambiguous phenotype. Here, we report the creation of a nonreverting strain of bacteria that produced statistical proteins. Ablating the editing activity of isoleucyl-tRNA synthetase resulted in an ambiguous code in which, through supplementation of a limited supply of isoleucine with an alternative amino acid that was noncoding, the mutant generating statistical proteins was favored over the wild-type isogenic strain. Such organisms harboring statistical proteins could have had an enhanced adaptive capacity and could have played an important role in the early development of living systems.
authors.affiliation: Evologic SA, 93 Rue Henri Rochefort, 91000 Evry, France.
authors.forename: V
authors.initials: V
authors.lastname: Pezo
authors.forename: D
authors.initials: D
authors.lastname: Metzgar
authors.forename: T L
authors.initials: TL
authors.lastname: Hendrickson
authors.forename: W F
authors.initials: WF
authors.lastname: Waas
authors.forename: S
authors.initials: S
authors.lastname: Hazebrouck
authors.forename: V
authors.initials: V
authors.lastname: Döring
authors.forename: P
authors.initials: P
authors.lastname: Marlière
authors.forename: P
authors.initials: P
authors.lastname: Schimmel
authors.forename: V
authors.initials: V
authors.lastname: De Crécy-Lagard
mesh_terms: D000215:Acylation; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D005798:Genes, Bacterial; D005815:Genetic Code; D007533:Isoleucine-tRNA Ligase / Q000235:genetics / Q000378:metabolism; D008957:Models, Genetic; D017393:RNA Editing
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D007533:Isoleucine-tRNA Ligase