Complementation of Saccharomyces cerevisiae ccc2 mutant by a putative P1B-ATPase from Brassica napus supports a copper-transporting function.
Copper transport across membranes plays an important role in plant growth and survival. P(1B)-type ATPases participate in transmembrane transport of copper in various organisms. A Brassica napus cDNA (BnRAN1) encoding a putative Cu(2+)-ATPase was cloned in this study. A complementation assay demonstrated that the protein encoded by this cDNA could functionally replace Ccc2p, a Saccharomyces cerevisiae Cu(2+)-ATPase, rescuing growth of ccc2 mutant under iron-limited conditions. Our results suggest that this rescue likely resulted from restoration of copper delivery, mediated by BnRAN1, to Fet3p. This study is amongst the first to demonstrate that a putative plant P(1B)-ATPase is functional and to examine its substrate specificity.
Southron
Jennafer L
JL
Department of Biological Sciences, University of Alberta, Edmonton, Canada T6G 2E9. jsouthro@ualberta.ca
Basu
Urmila
U
Taylor
Gregory J
GJ
D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000378:metabolism*; D001692:Biological Transport; D029688:Brassica napus / Q000201:enzymology* / Q000235:genetics; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism; D003300:Copper / Q000737:chemistry / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D005690:Galactose / Q000378:metabolism; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D005947:Glucose / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D011887:Raffinose / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D014170:Transformation, Genetic
D027682:Cation Transport Proteins; D018076:DNA, Complementary; D011994:Recombinant Proteins; D003300:Copper; D007501:Iron; D000251:Adenosine Triphosphatases; D005947:Glucose; D011887:Raffinose; D005690:Galactose