Beneficial effects of supplemental buffer and substrate on energy metabolism during small bowel storage.
Successful preservation of small bowel (SB) is closely correlated with the maintenance of cellular energetics. This study was designed to assess the ability of a modified UW solution supplemented with buffer and glucose to facilitate ATP production during cold storage. In part A, rats SB (n = 4) were flushed vascularly as follows: Group 1, UW solution (control); Group 2, HUW solution (UW+90 mM histidine). Inclusion of histidine resulted in a >3-fold increase in buffering capacity over the pH range 7.4-6.8. Positive effects of histidine on ATP and energy charge were apparent after 4-10h storage. Examination of the key regulatory enzyme, Phosphofructokinase (PFK), reflected a sustained activation was over 1-4h in the HUW group only. In part B, groups were vascularly flushed as follows: Group 1, HUW solution (control); Group 2, Group 1+20mM glucose; and Group 3, Group 2+luminal flush. Elevated ATP and total adenylates over 2-10h in Group 3 compared to control were a direct consequence of improved glycolytic activity. This data supports the hypothesis that tissue energetics can be significantly improved during cold storage using a histidine-buffered UW solution supplemented with carbohydrate substrate.
Salehi
Payam
P
Surgical-Medical Research Institute, 1074 Dentistry-Pharmacy Building, University of Alberta, Edmonton, Alta., Canada T6G 2N8.
Spratlin
Jennifer
J
Chong
Tze-Feng
TF
Churchill
Thomas A
TA
D000241:Adenosine; D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000493:Allopurinol; D000818:Animals; D002021:Buffers; D003080:Cold Temperature; D004734:Energy Metabolism / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D005978:Glutathione; D024981:Glycogen Phosphorylase / Q000378:metabolism; D006019:Glycolysis; D006639:Histidine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007328:Insulin; D007421:Intestine, Small / Q000378:metabolism*; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D025221:Phosphofructokinases / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D011887:Raffinose; D051381:Rats; D017207:Rats, Sprague-Dawley
D002021:Buffers; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D006639:Histidine; D000493:Allopurinol; D000255:Adenosine Triphosphate; D024981:Glycogen Phosphorylase; D025221:Phosphofructokinases; D011770:Pyruvate Kinase; D005978:Glutathione; D005947:Glucose; D000241:Adenosine; D011887:Raffinose