title: [Effect of granulocyte colony-stimulating factor on the ratio of peripheral blood dendritic cell subsets].
abstract: OBJECTIVE
  To clarify the effect of granulocyte colony-stimulating factor (G-CSF) on the radio of two peripheral blood dendritic cell(DC) subsets in-vivo.
  
  
  METHODS
  Various doses (0, 5, 10 and 15 microg) of G-CSF were subcutaneosly injected respectively into BALB/c mice, once each day, for 6 days running. Six days later, DCs were separated from peripheral blood. Then the flow cytometry was used to detect the radio of DC1 and DC2 (CD11c(+) CD8a(-) and CD11c(+) CD8a(+)), and to calculate their absolute numbers.
  
  
  RESULTS
  After stimulation with varying doses of G-CSF for 6 days, the absolute number of DC2s increased from 9.6x10(6)/L to 55.1x10(6)/L (P<0.01), while that of DC1s had no notably variation, so the ratio of DC1 and DC2 decreases from 4.2+/-1.1 to 0.7+/-0.3 (P<0.01).
  
  
  CONCLUSIONS
  G-CSF can increase the absolute number of peripheral blood DC2s, but has no influence on peripheral blood DC1 number, thus inversing the ratio of DC1/DC2 in-vivo.
authors.affiliation: Department of Hepato-biliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Jing-Shi
authors.initials: JS
authors.lastname: Zhou
authors.forename: Ke-Feng
authors.initials: KF
authors.lastname: Dou
authors.forename: Kai
authors.initials: K
authors.lastname: Sun
authors.forename: Rong
authors.initials: R
authors.lastname: Fu
authors.forename: Hai-Min
authors.initials: HM
authors.lastname: Li
authors.forename: Fu-Qin
authors.initials: FQ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001773:Blood Cells; D003713:Dendritic Cells / Q000276:immunology; D005434:Flow Cytometry; D016179:Granulocyte Colony-Stimulating Factor
chemical_list: D016179:Granulocyte Colony-Stimulating Factor