title: Hydrolysis of rice bran oil using an immobilized lipase from Candida rugosa in isooctane.
abstract: The kinetics of enzymatic hydrolysis of rice bran oil in isooctane by immobilized Candida rugosa lipase in a batch reactor showed competitive inhibition by isooctane with a dissociation constant, K1, of 0.92 M. Continuous hydrolysis of rice bran oil was performed in recycling, packed bed reactor with 4352 U of immobilized lipase; the optimum recycle ratio was 9 and the operational half-life was 360 h without isooctane but 288 h with 25% (v/v) isooctane in rice bran oil.
authors.affiliation: Department of Chemical Engineering, Manipal Institute of Technology, Manipal Academy of Higher Education (Deemed University), Manipal-576104, India. vytlarama@yahoo.com
authors.forename: V Ramachandra
authors.initials: VR
authors.lastname: Murty
authors.forename: Jayadev
authors.initials: J
authors.lastname: Bhat
authors.forename: P K A
authors.initials: PK
authors.lastname: Muniswaran
mesh_terms: D019149:Bioreactors; D002175:Candida / Q000201:enzymology*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D006868:Hydrolysis; D007700:Kinetics; D008049:Lipase / Q000737:chemistry*; D008956:Models, Chemical; D009795:Octanes / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D000073879:Rice Bran Oil
chemical_list: D004800:Enzymes, Immobilized; D009795:Octanes; D010938:Plant Oils; D008049:Lipase; D000073879:Rice Bran Oil; C045798:2,2,4-trimethylpentane