[Screening of TNF-alpha mimotopes from c7c phage display peptide library].
OBJECTIVE
To acquire oligo-peptides mimicking TNF-alpha epitopes.


METHODS
The TNF-alpha mimotopes were screened from c7c phage display peptide library by using neutralizing mAb J1D9 against TNF-alpha, and positive clones were identified by sandwich ELISA.


RESULTS
9 out of 20 phage clones were identified as positive clones which can bind to mAb J1D9. The deduced amino acid sequence analysis showed three different sequences: c-RRPAQSG-c, c-NKHNRKI-c and c-RGMSRKI-c.


CONCLUSIONS
All of the three peptides obtained can mimic the epitopes of TNF-alpha and bind to mAb J1D9.
Luo
Hai-Bo
HB
Department of Immunology, First Military Medical University, Guangzhou 510515, China.
Liu
Bei-Yi
BY
Liu
Yan-Jun
YJ
Zhu
Ping
P
Fu
Ning
N
D000595:Amino Acid Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D019151:Peptide Library; D010455:Peptides / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000939:Epitopes; D019151:Peptide Library; D010455:Peptides; D014409:Tumor Necrosis Factor-alpha