title: [In-vivo dynamic changes of antigen presenting cells infected with recombinant attenuated Salmonella typhimurium expressing green fluorescent protein].
abstract: OBJECTIVE
  To clarify in-vivo early dynamic changes of antigen-presenting cells (APCs) infected with recombinant attenuated Salmonella typhimurium.
  
  
  METHODS
  BALB/c mice were orally infected with recombinant attenuated Salmonella typhimurium expressing green fluorescent protein (GFP) X4550 (pYAGFP). Peritoneal macrophages were taken out 3 days after infection, cultured for 24 hours, and observed under the fluorescent microscope. Moreover, BALB/c mice were also infected intravenously with X4550 (pYAGFP) and low density cells (LDCs) were isolated and prepared from mouse spleen and liver 3,6 and 12 hours after infection. Infection rates of macrophages and dendritic cells (DCs) were analyzed by flow cytometry.
  
  
  RESULTS
  About 50% peritoneal macrophages were X4550 (pYAGFP) positive. Infection rates of macrophages in spleen and liver were about 20%-40%. As for the DCs, the infection rates in spleen and liver were about 4%-10% and 10%-20%, respectively.
  
  
  CONCLUSIONS
  Recombinant attenuated Salmonella can be captured by APCs in-vivo in early infection, which provides a precondition for inducing effective immune responses.
authors.affiliation: Key Laboratory of Animal Infectious Disease of Ministry of Agriculture, Yangzhou University, Yangzhou 225009, China. rwangfang@hotmail.com
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Xin-an
authors.initials: XA
authors.lastname: Jiao
authors.forename: Jian
authors.initials: J
authors.lastname: Gu
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Richard
authors.initials: R
authors.lastname: Lo-Man
authors.forename: Claude
authors.initials: C
authors.lastname: Leclerc
authors.forename: Xiu-fan
authors.initials: XF
authors.lastname: Liu
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000382:microbiology*; D005260:Female; D049452:Green Fluorescent Proteins; D008099:Liver / Q000382:microbiology; D008164:Luminescent Proteins / Q000235:genetics; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D012486:Salmonella typhimurium / Q000472:pathogenicity*; D013154:Spleen / Q000382:microbiology
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins