title: T-588, a cognitive enhancer, protects against sodium nitroprusside-induced toxicity in cultured astrocytes.
abstract: The effects of (1R)-1-benzo[b]thiophen-5-yl-2-[2-(diethylamino)ethoxy]ethan-1-ol hydrochloride (T-588), a cognitive enhancer, on sodium nitroprusside (SNP)-induced cytotoxicity were examined in cultured rat astrocytes. Treatment with 100 microM SNP for 72 h decreased cell viability and mitochondrial function assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenil tetrazolium bromide (MTT) reduction activity, mitochondrial transmembrane potential, and intracellular ATP level. T-588 at 100 microM prevented SNP-induced mitochondrial dysfunction and cell injury. Furthermore, T-588 increased MTT reduction activity without affecting cell proliferation in astrocytes. These results suggest that T-588 has a protective effect against SNP-mediated toxicity via improvement of mitochondrial dysfunction in astrocytes.
authors.affiliation: Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Japan.
authors.forename: Patamawan
authors.initials: P
authors.lastname: Phuagphong
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Fukushima
authors.forename: Rie
authors.initials: R
authors.lastname: Hatanaka
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Tanaka
authors.forename: Akemichi
authors.initials: A
authors.lastname: Baba
authors.forename: Toshio
authors.initials: T
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D004048:Diethylamines / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009599:Nitroprusside / Q000633:toxicity*; D018697:Nootropic Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013876:Thiophenes / Q000494:pharmacology*
chemical_list: D004048:Diethylamines; D018696:Neuroprotective Agents; D018697:Nootropic Agents; D013876:Thiophenes; C082372:T 588; D009599:Nitroprusside