A highly discriminating quencher-free molecular beacon for probing DNA.
We inserted a fluorene-labeled deoxyuridine derivative, synthesized using Sonogashira coupling, efficiently into the loop region of a DNA hairpin using phosphoramidite chemistry. This molecular beacon, which features no additional fluorescence quencher, discriminates between perfect and one-base-mismatched base pairing by changes in its fluorescence intensity. The discrimination factor is 14.7 for the recognition of a single (A/C) base mismatch.
Hwang
Gil Tae
GT
Department of Chemistry, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea.
Seo
Young Jun
YJ
Kim
Byeang Hyean
BH
D020137:Base Pair Mismatch; D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D005449:Fluorenes / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009705:Nucleosides / Q000138:chemical synthesis; D009841:Oligonucleotides / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013696:Temperature
D005449:Fluorenes; D009705:Nucleosides; D009841:Oligonucleotides; D004247:DNA