Determining virological, serological and immunological parameters of EBV infection in the development of PTLD.
Post-transplant lymphoproliferative disease (PTLD) in Epstein-Barr virus (EBV) seronegative solid organ transplant recipients remains a significant problem, particularly in the first year post-transplant. Immune monitoring of a cohort of high-risk patients indicated that four EBV seronegative transplant recipients developed early-onset PTLD prior to evidence of an EBV humoral response. EBV status has been classically defined serologically, however these patients demonstrated multiple parameters of EBV infection, including the generation of EBV-specific CTL, outgrowth of spontaneous lymphoblastoid cell lines, and elevated EBV DNA levels, despite the absence of a classic EBV antibody response. As EBV serology is influenced by both immunosuppression and cytomegalovirus immunoglobulin treatment, both the EBV-specific CTL response and elevated EBV levels are more reliable indicators of EBV infection post-transplant.
Davis
Joanne E
JE
Division of Immunology and Infectious Diseases and the Cooperative Research Centre for Vaccine Technology, The Queensland Institute of Medical Research and University of Queensland Joint Oncology Program, Brisbane, 4029 Australia. joanne.Da@qimr.edu.au
Sherritt
Martina A
MA
Bharadwaj
Mandvi
M
Morrison
Leanne E
LE
Elliott
Suzanne L
SL
Kear
Laurie M
LM
Maddicks-Law
Joanne
J
Kotsimbos
Tom
T
Gill
Devinder
D
Malouf
Monique
M
Falk
Michael C
MC
Khanna
Rajiv
R
Moss
Denis J
DJ
D000293:Adolescent; D000328:Adult; D004279:DNA, Viral / Q000097:blood*; D020031:Epstein-Barr Virus Infections / Q000097:blood / Q000175:diagnosis / Q000276:immunology* / Q000628:therapy / Q000821:virology; D005260:Female; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007116:Immunization, Passive; D007165:Immunosuppression Therapy; D016031:Liver Transplantation; D016040:Lung Transplantation; D008232:Lymphoproliferative Disorders / Q000175:diagnosis / Q000209:etiology / Q000276:immunology* / Q000821:virology; D008297:Male; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000821:virology
D004279:DNA, Viral