title: Characterization of the glycosyltransferase activity of desVII: analysis of and implications for the biosynthesis of macrolide antibiotics.
abstract: In vitro catalytic activity of DesVII, the glycosyltransferase involved in the biosynthesis of methymycin, neomethymycin, narbomycin, and pikromycin in Streptomyces venezuelae, is described. This is the first report of demonstrated in vitro activity of a glycosyltransferase involved in the biosynthesis of macrolide antibiotics. DesVII is unique among glycosyltransferases in that it requires an additional protein component, DesVIII, as well as basic pH for its full activity.
authors.affiliation: Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712, USA.
authors.forename: Svetlana A
authors.initials: SA
authors.lastname: Borisova
authors.forename: Lishan
authors.initials: L
authors.lastname: Zhao
authors.forename: Charles E
authors.initials: CE
authors.lastname: Melançon III
authors.forename: Chai-Lin
authors.initials: CL
authors.lastname: Kao
authors.forename: Hung-Wen
authors.initials: HW
authors.lastname: Liu
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D006031:Glycosylation; D016695:Glycosyltransferases / Q000378:metabolism*; D018942:Macrolides / Q000378:metabolism*; D015394:Molecular Structure; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D018942:Macrolides; C008517:methymycin; D016695:Glycosyltransferases; C003145:picromycin