The effects of interferon-gamma on the expression of the cyclin D isoforms in cord blood hematopoietic stem/progenitor cells.
To explore the hematopoiesis inhibition mechanisms of interferon-gamma (IFN-gamma), the effects of IFN-gamma on the expression of the cyclin D in the umbilical cord blood hematopoietic stem/progenitor cells were observed. In the experiments the CD34(+) cells were isolated from the cord blood with MIDI-MACS system; semi-solid methylcellulose culture technique was used to measure the formation of CFU-GM; the expression levels of cyclin D isoforms were assayed by semi-quantitative RT-PCR, after the hematopoietic stem/progenitor cells were incubated with IFN-gamma. The results indicated that IFN-gamma could inhibit the formation of CFU-GM and down-regulate the expression of cyclin D2 and cyclin D3 at the mRNA level. It is concluded that the IFN-gamma could inhibit the proliferation of hematopoietic stem cells and down-regulate the expression of cyclin D, that may be one mechanism underlying the hematopoietic inhibition of IFN-gamma.
Meng
Fan-Kai
FK
Department of Hematology, Tongji hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Tan
Xi-You
XY
Liu
Wen-Li
WL
Sun
Han-Ying
HY
Zhou
Jian-Feng
JF
Zhou
Yin-Li
YL
Wu
Ning
N
Sun
Lan
L
D056741:Cyclin D; D016213:Cyclins / Q000235:genetics*; D005312:Fetal Blood / Q000166:cytology*; D016193:G1 Phase; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000032:analysis
D056741:Cyclin D; D016213:Cyclins; D020033:Protein Isoforms; D012333:RNA, Messenger; D007371:Interferon-gamma