Strain improvement for cephalosporin production by Acremonium chrysogenum using geneticin as a suitable transformation marker.
An Acremonium chrysogenum strain improvement program based on the transformation with cephalosporin biosynthetic genes was carried out to enhance cephalosporin C production. Best results were obtained with cefEF and cefG genes, selecting transformants with increased cephalosporin C production and lower accumulation of biosynthetic intermediates. Phleomycin resistant transformants, designated B1 and C1, showed a single copy random integration event, higher levels of cefEF transcript and, according to immunoblotting analyses, higher amounts of deacetylcephalosporin C acetyltransferase (DAC-AT) protein than their parental strains. Moreover, DAC-AT activity was higher in the transformants. Plasmids carrying geneticin resistance markers based on the nptII gene from Tn5 and the aphI gene from Tn903 were constructed to transform again B1 and C1, showing that the cassette Pgdh-nptII-trpC was able to confer geneticin resistance to A. chrysogenum and demonstrating that geneticin is a helpful selection marker.
Rodríguez-Sáiz
Marta
M
R+D Biology, Antibióticos S.A., Avenida de Antibióticos 59-61, 24009 León, Spain.
Lembo
Marianna
M
Bertetti
Luca
L
Muraca
Roberto
R
Velasco
Javier
J
Malcangi
Antonella
A
de la Fuente
Juan Luis
JL
Barredo
José Luis
JL
D000164:Acremonium / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D002511:Cephalosporins / Q000096:biosynthesis*; D024881:Drug Resistance, Bacterial; D005798:Genes, Bacterial; D005819:Genetic Markers; D005839:Gentamicins / Q000494:pharmacology*; D014170:Transformation, Genetic
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D005819:Genetic Markers; D005839:Gentamicins; C025163:cephalosporin C; C010680:antibiotic G 418