title: Induction of secretory and tumoricidal activities in peritoneal macrophages activated by an acidic heteropolysaccharide (ARAGAL) from the gum of Anadenanthera colubrina (Angico branco).
abstract: The immunomodulatory and anti-tumoral effects of an acidic heteropolysaccharide containing mainly galactose and arabinose (ARAGAL), isolated from the gum of the leguminous tree Anadenanthera colubrina (Angico branco) native to Brazil, were studied. It has been demonstrated that activation of mice peritoneal macrophages both in vivo and in vitro, increases phagocytic ability and anion superoxide production. In order to obtain further insights on the biological effects of ARAGAL, the capacity of eliciting peritoneal macrophages and tumor necrosis factor-alpha (TNF-alpha) production, and anti-tumoral effect against Sarcoma 180 (S-180), are now evaluated. Cell eliciting activity was observed in ARAGAL-treated animals in a dose dependent manner. Treatment of animals with 50, 100 or 200 mg/kg of ARAGAL increased peritoneal exudate cell (PEC) numbers by approximately 18, approximately 44 and approximately 88%, respectively. ARAGAL also increased 26-fold TNF-alpha production by peritoneal macrophages. Macrophages, treated in vitro for 18 h with ARAGAL, were able to kill Sarcoma 180 cells, as observed by their structures inside the macrophage cytoplasm. ARAGAL (100 mg/kg) showed anti-tumoral activity against S-180 in ascites or solid tumors, the tumoral inhibition being 63 and 38%, respectively. The results suggest a possible role as a BRM for ARAGAL.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, CEP 81531-990 Curitiba, Paraná, PR, Brazil.
authors.forename: Mariana Piemonte
authors.initials: MP
authors.lastname: Moretão
authors.forename: Aleksander Roberto
authors.initials: AR
authors.lastname: Zampronio
authors.forename: Philip A J
authors.initials: PA
authors.lastname: Gorin
authors.forename: Marcello
authors.initials: M
authors.lastname: Iacomini
authors.forename: Maria Benigna M
authors.initials: MB
authors.lastname: Oliveira
mesh_terms: D000818:Animals; D001202:Ascitic Fluid / Q000187:drug effects / Q000473:pathology; D001938:Brazil; D002240:Carbohydrate Sequence; D002452:Cell Count; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D003602:Cytotoxicity, Immunologic / Q000502:physiology; D004354:Drug Screening Assays, Antitumor; D007887:Fabaceae / Q000737:chemistry*; D005685:Galactans / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D008262:Macrophage Activation / Q000276:immunology* / Q000502:physiology; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D009369:Neoplasms / Q000188:drug therapy / Q000473:pathology; D010529:Peritoneal Cavity / Q000166:cytology; D011134:Polysaccharides / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D005685:Galactans; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; C005653:arabinogalactan