title: Determination of mangiferin, jateorrhizine, palmatine, berberine, cinnamic acid, and cinnamaldehyde in the traditional Chinese medicinal preparation Zi-Shen pill by high-performance liquid chromatography.
abstract: High-performance liquid chromatography is employed to determine the contents of six marker components such as mangiferin, jateorrhizine, palmatine, berberine, cinnamic acid, and cinnamaldehyde in the traditional Chinese medicinal preparation Zi-Shen pill. The separation is performed on a C(18) column by stepwise gradient elution with water (0.2%, v/v, triethylamine adjusted to pH 4 with phosphoric acid)-methanol-acetonitrile (0.01 min, 98:0:2; 20 min, 80:5:15; 30 min, 65:13:22; and 55 min, 65:13:22) as the mobile phase at a flow rate of 0.9 mL/min, with UV detection at 280 nm. Six regression equations show good linear relationships between the peak area of each marker and concentration. The recoveries of the markers listed are 95.5%, 98.3%, 96.8%, 99.5%, 101.7%, and 102.1%, respectively. The repeatability and reproducibility (relative standard deviation) of the method are less than 2.5% and 3.3%, respectively.
authors.affiliation: Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, PR China.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Dai
authors.forename: Kang
authors.initials: K
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Kaishun
authors.initials: K
authors.lastname: Bi
mesh_terms: D000171:Acrolein / Q000031:analogs & derivatives* / Q000032:analysis; D001599:Berberine / Q000032:analysis; D001600:Berberine Alkaloids / Q000032:analysis; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002934:Cinnamates / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D044004:Xanthones / Q000032:analysis
chemical_list: D001600:Berberine Alkaloids; D002934:Cinnamates; D004365:Drugs, Chinese Herbal; D044004:Xanthones; D001599:Berberine; C029010:cinnamic acid; C013592:mangiferin; D000171:Acrolein; C005413:palmatine; C012843:cinnamaldehyde