Power-free poly(dimethylsiloxane) microfluidic devices for gold nanoparticle-based DNA analysis.
An extremely simple, power-free pumping method for poly(dimethylsiloxane)(PDMS) microfluidic devices is presented. By exploiting the high gas solubility of PDMS, the energy for the pumping is pre-stored in the degassed bulk PDMS, therefore no additional structures other than channels and reservoirs are required. In a Y-shaped microchannel with cross section of 100 microm width x 25 microm height, this method has provided flow rate of 0.5-2 nL s(-1), corresponding to linear velocity of 0.2-0.8 mm s(-1), with good reproducibility. As an application of the power-free pumping, gold nanoparticle-based DNA analysis, which does not rely on the cross-linking mechanism between nanoparticles, has been implemented in a microchannel with three inlets. Target 15mer DNA has been easily and unambiguously discriminated from its single-base substituted mutant. Instead of colorimetric detection in a conventional microtube, an alternative detection technique suitable for microdevices has been discovered-observation of deposition on the PDMS surfaces. The channel layout enabled two simultaneous DNA analyses at the two interfaces between the three laminar streams.
Hosokawa
Kazuo
K
Bioengineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. k-hoso@riken.go.jp
Sato
Kae
K
Ichikawa
Naoki
N
Maeda
Mizuo
M
D004247:DNA / Q000032:analysis*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D044085:Microfluidics / Q000379:methods; D049329:Nanostructures; D012828:Silicones / Q000737:chemistry*; D013997:Time Factors
D004129:Dimethylpolysiloxanes; D012828:Silicones; C013830:baysilon; D006046:Gold; D004247:DNA