Alterations in monoamine levels after cocaine-induced status epilepticus and death in striatum and prefrontal cortex of mice.
Seizures and death are the more important toxic consequences related to cocaine overdose. Some reports have shown that pharmacological manipulations in dopaminergic, serotonergic and noradrenergic systems alter the occurrence of cocaine-induced convulsions and death. Based on this fact, this work was performed to determine the changes in monoamine levels (DA, 5-HT and NE) and their metabolites (DOPAC, HVA and 5-HIAA) after cocaine-induced status epilepticus (SE) and death in striatum and prefrontal cortex (PFC). The monoamines and their metabolites were assayed by reverse-phase high-performance liquid chromatography with electrochemical detection. Animal SE in striatum presented a decrease in DA and NE levels and an increase in HVA although in PFC there was an increase in DA, 5-HT and NE. Animals that died from cocaine-induced seizures in striatum showed an increase only in NE levels, but on the other hand in PFC a decrease occurred in DA and NE levels. Taken together these results indicated that cocaine-induced SE and death altered monoamine levels in different ways depending on the brain area studied, suggesting that different mechanisms are involved.
Macêdo
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DS
Department of Physiology and Pharmacology, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, Fortaleza 60431-970, CE, Brazil.
Vasconcelos
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SM
Belchior
L D
LD
Santos
R S
RS
Viana
G S B
GS
Sousa
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FC
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D015306:Biogenic Monoamines / Q000378:metabolism*; D001923:Brain Chemistry; D002452:Cell Count / Q000379:methods; D016923:Cell Death / Q000187:drug effects / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003042:Cocaine; D003342:Corpus Striatum / Q000378:metabolism*; D004563:Electrochemistry / Q000379:methods; D006719:Homovanillic Acid / Q000378:metabolism; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D008297:Male; D051379:Mice; D017397:Prefrontal Cortex / Q000378:metabolism*; D013226:Status Epilepticus / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology*
D015306:Biogenic Monoamines; D015102:3,4-Dihydroxyphenylacetic Acid; D006897:Hydroxyindoleacetic Acid; D003042:Cocaine; D006719:Homovanillic Acid