title: Epithelio-mesenchymal transition in a neoplastic ovarian epithelial hybrid cell line.
abstract: A hybrid cell line, IOSE-Ov29, was created through fusion of cells from the human ovarian adenocarcinoma line OVCAR3 and the non-tumorigenic SV40 Tag-transfected human ovarian surface epithelial line IOSE-29. OVCAR3 cells exhibit a differentiated epithelial phenotype, whereas line IOSE-29 expresses mesenchymal characteristics that were acquired in culture by epithelio-mesenchymal transition. Microsatellite analysis, comparative genomic hybridization (CGH), and MFISH showed the genotype of the IOSE-Ov29 cells to contain components of both parent cell lines, but to be predominantly OVCAR3 derived. IOSE-Ov29 resembled OVCAR3 and differed from IOSE-29 as shown by its unlimited life span, tumorigenicity, epithelial morphology, keratin, occludin, E-cadherin and CA125 expression, increased expression of kinases of the PI3K pathway, and loss of cGMP-dependent protein kinase expression. IOSE-29-derived properties included SV40 Tag expression, growth inhibition by activin, collagen type III secretion, increased adhesion and spreading on tissue culture plastic, and increased growth rate. Proliferation of all three lines was stimulated by FSH and ATP and inhibited by GnRH I and GnRH II. Interestingly, IOSE-Ov29 was more anchorage independent than either parent line and was the only line that invaded Matrigel in Boyden chambers and formed invasive branches in collagen gels. The results indicate that IOSE-Ov29 is an IOSE-29/OVCAR3 hybrid, which differs from both parent lines genetically and phenotypically. Unexpectedly, fusion with the non-tumorigenic IOSE-29 cells enhanced malignancy-associated characteristics of OVCAR3, presumably as a result of the expression of IOSE-29-derived mesenchymal properties that are usually acquired by carcinoma cells through epithelio-mesenchymal transition during metastatic progression.
authors.affiliation: Department of Obstetrics and Gynaecology, University of British Columbia, BC Women's Hospital, 4490 Oak Street, Vancouver, BC V6H 3V5, Canada.
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Maines-Bandiera
authors.forename: David
authors.initials: D
authors.lastname: Huntsman
authors.forename: Valia S
authors.initials: VS
authors.lastname: Lestou
authors.forename: Wen-Lin
authors.initials: WL
authors.lastname: Kuo
authors.forename: Peter C K
authors.initials: PC
authors.lastname: Leung
authors.forename: R Douglas
authors.initials: RD
authors.lastname: Horsman
authors.forename: Alice S T
authors.initials: AS
authors.lastname: Wong
authors.forename: Michelle M M
authors.initials: MM
authors.lastname: Woo
authors.forename: Keith K C
authors.initials: KK
authors.lastname: Choi
authors.forename: Calvin D
authors.initials: CD
authors.lastname: Roskelley
authors.forename: Nelly
authors.initials: N
authors.lastname: Auersperg
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000483:Alleles; D000952:Antigens, Polyomavirus Transforming / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D015820:Cadherins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D024061:Collagen Type III / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D046508:Culture Techniques; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D006822:Hybrid Cells; D007621:Karyotyping; D008648:Mesoderm / Q000378:metabolism / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D018911:Trinucleotide Repeats / Q000235:genetics
chemical_list: D000952:Antigens, Polyomavirus Transforming; D015820:Cadherins; D024061:Collagen Type III