Brain metabolite concentration ratios in vivo: multisite reproducibility by single-voxel 1H MR spectroscopy.
Ten normal subjects were scanned identically at three separate sites (Little Rock, Houston, and New Orleans) to evaluate the reproducibility of brain metabolite ratios in single-voxel (1)H point-resolved spectroscopy sequence (PRESS) magnetic resonance (MR) spectroscopy in vivo. All scans were processed by a single individual at a single site. Coefficients of variation of the measured metabolite ratios generally were in the range found for previous single-voxel, single-site reproducibility studies. No differences were found among the sites for ratios of N-acetylaspartate to creatine (NAA/Cr) or choline to Cr (Cho/Cr) in left thalamus by multivariate ANOVA. Metabolite ratios of Cr or Cho relative to local brain H(2)O did not vary among the sites. However, by multivariate ANOVA, NAA/H(2)O differed between Little Rock and New Orleans, but not between those sites and Houston. Intraclass correlation coefficients suggested reasonable reproducibility between Little Rock and New Orleans, but not between those sites and Houston.
Komoroski
Richard A
RA
Department of Radiology, University of Arkansas for Medical Sciences, Little Rock 72205, USA. komoroskiricharda@uams.edu
Kotrla
Kathryn J
KJ
Lemen
Lisa
L
Lindquist
Diana
D
Diaz
P
P
Foundas
Anne
A
D000328:Adult; D000704:Analysis of Variance; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D002794:Choline / Q000378:metabolism; D015331:Cohort Studies; D003404:Creatinine / Q000378:metabolism; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D013788:Thalamus / Q000378:metabolism*
D001224:Aspartic Acid; C000179:N-acetylaspartate; D003404:Creatinine; D002794:Choline