title: Presence of TT virus DNA in bone marrow cells from hematologic patients.
abstract: Recent observations suggest that TT virus (TTV), in addition to liver, may also infect bone marrow. In this study, bone marrow samples and sera from 33 patients with haematological disorders and sera from 16 healthy controls were investigated for TTV DNA presence. Altogether TTV DNA sequences were demonstrated in bone marrow cells of 84.84% of patients. Moreover TTV DNA was detected in sera from 72.72% of patients and from 93.75% of controls. N22 sequences amplified from bone marrow cells and serum of 3 patients were analysed, after cloning: all these isolates were of type 2c and 2 or 3 variants were present in each isolate. After single strand DNA degradation, replicative forms were detectable in BM cells. This finding, in addition to the detection of variants similar in the BM and in the serum of the same patient could suggest that BM is a site of TTV replication (or one of the sites) from which the virus is spread in blood.
authors.affiliation: Haematology Unit, University of Florence and Careggi Hospital of Florence, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Fanci
authors.forename: R
authors.initials: R
authors.lastname: De Santis
authors.forename: K
authors.initials: K
authors.lastname: Zakrzewska
authors.forename: C
authors.initials: C
authors.lastname: Paci
authors.forename: A
authors.initials: A
authors.lastname: Azzi
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D001854:Bone Marrow Cells / Q000821:virology*; D004266:DNA Virus Infections / Q000150:complications* / Q000175:diagnosis* / Q000276:immunology; D004277:DNA, Single-Stranded / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D005838:Genotype; D006402:Hematologic Diseases / Q000150:complications*; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000150:complications; D008875:Middle Aged; D009101:Multiple Myeloma / Q000150:complications; D010265:Paraproteinemias / Q000150:complications; D013921:Thrombocytopenia / Q000150:complications; D022783:Torque teno virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000914:Antibodies, Viral; D004277:DNA, Single-Stranded; D004279:DNA, Viral