Inhibitory effect of cadmium and tobacco alkaloids on expansion of porcine oocyte-cumulus complexes.
Studies aimed at the influence of smoking on reproductive functions have found out fertility disorders in smokers occurring at any stage of reproductive processes. In our experiments the role of cadmium, nicotine and anabasine was investigated in the expansion of oocyte-cumulus complexes (OCC) isolated from large antral porcine follicles. Suppression of FSH-induced cumulus expansion and significant inhibition of synthesis and accumulation of hyaluronic acid in the cell/matrix compartment of the OCC was observed in the presence of different concentrations of tested compounds. The suppressive effect of cadmium and tobacco alkaloids on the cumulus expansion was accompanied by decreased progesterone production by cumulus cells during 42 h incubation of the OCC with FSH.
Mlynarcíková
A
A
Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic. ueenamly@savba.sk
Scsuková
S
S
Vrsanská
S
S
Nagyová
E
E
Ficková
M
M
Kolena
J
J
D000470:Alkaloids / Q000494:pharmacology*; D000691:Anabasine / Q000494:pharmacology*; D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D009538:Nicotine / Q000494:pharmacology*; D006080:Ovarian Follicle / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D013552:Swine
D000470:Alkaloids; D002104:Cadmium; D009538:Nicotine; D005640:Follicle Stimulating Hormone; D006820:Hyaluronic Acid; D000691:Anabasine