[Effects of LFA-1 costimulation on proliferation and IgG production of PBMCs from lupus nephritis patients].
OBJECTIVE
To study the effect of lymphocyte function associated antigen-1(LFA-1) costimulation on proliferation and immunoglobin production of peripheral blood mononuclear cells(PBMCs) form lupus nephritis(LN) patients.


METHODS
29 LN patients were enrolled in this study, and 12 healthy persons served as control. PBMCs from LN patients and healthy persons were obtained by Ficoll density gradient centrifugation, and cell proliferation was detected by (3)H-TdR incorporation. IgG content in cultural supermatant was detected by ELISA.


RESULTS
Stimulation of anti-CD3 mAb alone could enhance the proliferation and IgG production of PBMCs from 29 LN patients,while the effects on PBMCs from patients in active phase were stronger than those from the patients in the inactive phase (P<0.01). But anti-CD3 mAb had no influenence on PBMCs from healthy persons.The costimulation of anti-CD3 mAb and LFA-1 could increase proliferation and IgG production of PBMCs from LN patients and healthy persons. The effects of this costimulation decreased in turn from active and inactive LN patients to normal persons (P<0.01). The costimulant effects of LFA-1 was inhibited by anti-LFA-1 mAb.


CONCLUSIONS
The effects of enhancing PBMC proliferation and IgG production by LFA-1 may be a mechanism of LN pathogenesis.
Wang
Jian-Qin
JQ
Department of Nephrology, Sun Yat-sen University of Medical Sciences, Guangzhou 510080,China.
Li
You-Ji
YJ
Xia
Yan-Ling
YL
Ye
Ren-Gao
RG
Zhang
Di-Hua
DH
Zhou
Dao-Yuan
DY
D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008181:Lupus Nephritis; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1
D007074:Immunoglobulin G; D007376:Interleukin-2; D016169:Lymphocyte Function-Associated Antigen-1