title: Serial bull cloning by somatic cell nuclear transfer.
abstract: Although the list of species successfully cloned continues to grow, serial cloning has not been reported in species other than the mouse. Here we describe two live births of second-generation clones of a bull. Clones of the first and second generations appear healthy and have normal telomere lengths. Our attempts to produce the third generation of clones were unsuccessful.
authors.affiliation: Laboratory of Cell Genetics and Embryo Transfer, Kagoshima Prefectural Cattle Breeding Development Institute, Japan.
authors.forename: Chikara
authors.initials: C
authors.lastname: Kubota
authors.forename: X Cindy
authors.initials: XC
authors.lastname: Tian
authors.forename: Xiangzhong
authors.initials: X
authors.lastname: Yang
mesh_terms: D000034:Abortion, Veterinary; D000818:Animals; D001757:Blastomeres / Q000166:cytology; D002417:Cattle; D002452:Cell Count; D002970:Cleavage Stage, Ovum / Q000166:cytology; D019976:Cloning, Organism / Q000379:methods*; D004624:Embryo Transfer / Q000662:veterinary; D005260:Female; D005298:Fertility / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism; D008297:Male; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D019098:Telomerase / Q000235:genetics / Q000378:metabolism; D016615:Telomere / Q000235:genetics / Q000378:metabolism
chemical_list: D019098:Telomerase