title: Increased nuclear factor-kB activation in human colorectal carcinoma and its correlation with tumor progression.
abstract: OBJECTIVE
  Nuclear factor-kB (NF-kB) is a transcription factor that participates in the induction of several genes for cytokines and enzymes that play important functional roles in various cell types. The aim of this study was to determine NF-kB activation status in human colorectal carcinoma and its correlation to the clinicopathological characteristics of patients.
  
  
  METHODS
  We examined the activation status of NF-kB in 28 resected colorectal carcinomas and in colonic mucosa from uninvolved portions of these specimens by electrophoretic mobility shift assay (EMSA) and immunohistochemical staining for the p65 subunit of NF-kB.
  
  
  RESULTS
  EMSA showed much greater activation in the tumors than in normal mucosa, as did epithelial p65 immunostaining. NF-kB activation significantly increased in the more progressed cases (T3 + T4 cases or Stage II< cases). In vitro studies using lipopolysaccharide (LPS)-responsive colon carcinoma cells suggested a correlation between NF-kB activation and cell proliferation.
  
  
  CONCLUSIONS
  Our findings indicated that NF-kB is constitutively activated in human colorectal carcinoma tissue and correlates with tumor progression. The regulation of this transcription factor might be therapeutically useful against these tumors.
authors.affiliation: Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. s1kojima@surg1.med.kyushu-u.ac.jp
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kojima
authors.forename: Takashi
authors.initials: T
authors.lastname: Morisaki
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Sasaki
authors.forename: Kenji
authors.initials: K
authors.lastname: Nakano
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Mibu
authors.forename: Masao
authors.initials: M
authors.lastname: Tanaka
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Katano
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002455:Cell Division / Q000502:physiology; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D018450:Disease Progression; D004586:Electrophoresis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000096:biosynthesis / Q000378:metabolism*; D051996:Transcription Factor RelA
chemical_list: D016328:NF-kappa B; D051996:Transcription Factor RelA