Stage-specific expression of two actin genes in the yellow fever mosquito, Aedes aegypti.
Abstract The expression patterns of two muscle-specific actin genes were studied in the yellow fever mosquito, Aedes aegypti. The coding sequence of AeAct-2 exhibits between 82 and 85% similarity with coding sequences of the Drosophila melanogaster and predicted Anopheles gambiae actin genes. The transcription of the AeAct-2 gene was differentially regulated during developmental stages with higher levels of expression in larvae and lower levels in pupae and adults. The AeAct-2 gene is mainly expressed in the head and body wall tissues. Transcripts of the AeAct-3 gene are not detectable in larvae until late 4th instar and the level increased in male pupae and early male adults. The main site of expression of the AeAct-3 gene was the thoracic tissue. Thus, AeAct-3 is the first reported male-specific actin gene in mosquitoes.
Vyazunova
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Department of Entomology, University of Wisconsin-Madison, Madison, WI, USA.
Lan
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D000199:Actins / Q000235:genetics* / Q000378:metabolism*; D000330:Aedes / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D016000:Cluster Analysis; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004330:Drosophila / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D008018:Life Cycle Stages / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012737:Sex Factors
D000199:Actins; D017931:DNA Primers; D018076:DNA, Complementary