title: [Effects of the escharectomy during burn shock stage on expression of glucose translator-4 mRNA in skeletal muscle and adipose tissue].
abstract: OBJECTIVE
  To investigate the effect of the escharectomy during burn shock stage on expression of glucose translator-4 (GLUT4) mRNA in skeletal muscle and adipose tissue.
  
  
  METHODS
  30% TBSA scalded rats were employed. Escharectomy were conducted at 8 h, 24 h, 168 h after burns respectively. Insulin, glucagon, cortisol and glucose levels in serum were analyzed. RT-PCR were employed to analyze GLUT4 mRNA expression in skeletal muscle and adipose tissue.
  
  
  RESULTS
  Glucagon, cortisol and glucose levels in serum were declined in groups which escharectomy were conducted during burn shock stage. GLUT4 mRNA expression in both skeletal muscle and adipose tissue were downregulated after burns and escharectomy conducted during burn shock stage made it restored to near normal.
  
  
  CONCLUSIONS
  GLUT4 mRNA expression will declined after major burns in skeletal muscle and adipose tissue. Escharectomy during shock stage could make it upregulated, which will be helpful to improve glucose metabolism and hypermetabolism after major burns.
authors.affiliation: Burns department, Hospital of Armed Police in Sichuan, Leshan 614000, China.
authors.forename: Xiu-rong
authors.initials: XR
authors.lastname: Shuai
authors.forename: Tong-fa
authors.initials: TF
authors.lastname: Liu
authors.forename: Zhen-rong
authors.initials: ZR
authors.lastname: Guo
authors.forename: Shun-xian
authors.initials: SX
authors.lastname: Yu
authors.forename: Peng-fei
authors.initials: PF
authors.lastname: He
authors.forename: Wen-zhou
authors.initials: WZ
authors.lastname: Yuan
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Li-xin
authors.initials: LX
authors.lastname: He
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose; D002056:Burns / Q000503:physiopathology / Q000601:surgery*; D015870:Gene Expression; D005934:Glucagon / Q000097:blood; D006854:Hydrocortisone / Q000097:blood; D007328:Insulin / Q000097:blood; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D018482:Muscle, Skeletal / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012774:Shock, Traumatic / Q000503:physiopathology
chemical_list: D001786:Blood Glucose; D007328:Insulin; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; D005934:Glucagon; D006854:Hydrocortisone