title: [Inhibition of intracellular Ca(2+)-chelating agent on Hela cell apoptosis induced by HSV-1].
abstract: OBJECTIVE
  To explore the variation of intracellular free-Ca (2+) concentration during Hela cell apoptosis induced by HSV-1 and inhibition of Ca(2+)-chelating agent on the apoptosis.
  
  
  METHODS
  Apoptosis of the Hela cells infected by HSV-1 was observed under scanning electron microscope (SEM) and transmission electron microscope (TEM), and the variation of intracellular free-Ca (2+) concentration, labeled with fluorescent probe was observed under fluorescence-microscope at a series of timepoints.
  
  
  RESULTS
  The Hela cells infected by HSV-1 showed typical apoptotic morphological changes. Free-Ca (2+) concentration increased and reached peak at the 12 hours after HSV-1 infection. Characteristic morphological features of apoptosis occurred at the 24 hours after infection. This apoptosis could be inhibited by intracellular Ca(2+)-chelating agent.
  
  
  CONCLUSIONS
  Intracellular free-Ca(2+) might play an important role in Hela cell apoptosis induced by HSV-1, which provides useful clues for clinical treatment of associated diseases.
authors.affiliation: Department of Immunology, Harbin Medical University, Harbin 150086, China. yangqx_9051@yahoo.com
authors.forename: Qiu-xia
authors.initials: QX
authors.lastname: Yang
authors.forename: Hong-chi
authors.initials: HC
authors.lastname: Jiang
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Xue-yan
authors.initials: XY
authors.lastname: Xi
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
authors.forename: Xue-ying
authors.initials: XY
authors.lastname: Lu
authors.forename: Jin-yu
authors.initials: JY
authors.lastname: Gu
authors.forename: Dian-jun
authors.initials: DJ
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000502:physiology*; D002614:Chelating Agents / Q000494:pharmacology*; D006367:HeLa Cells; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D006801:Humans; D008854:Microscopy, Electron
chemical_list: D002614:Chelating Agents; D002118:Calcium