title: Ischemic preconditioning decreases intracellular zinc accumulation induced by oxygen-glucose deprivation in gerbil hippocampal CA1 neurons.
abstract: In normal gerbils, intracellular zinc ions ([Zn2+]i) and calcium ions ([Ca2+]i) accumulate in hippocampal CA1 neurons after global ischemia. We examined whether ischemic preconditioning modifies these changes in gerbil hippocampal slices. In normal slices, large increases in [Zn2+]i and [Ca2+]i were observed in the stratum radiatum of the CA1 area after oxygen-glucose deprivation. In preconditioned slices, there were significantly decreased peak levels of [Zn2+]i and [Ca2+]i in CA1. However, there were no differences in the peak levels of these ions in CA3 and dentate gyrus. These results suggest that modified [Zn2+]i and [Ca2+]i accumulation after an ischemic insult might be important for the mechanisms of ischemic tolerance induced by preconditioning.
authors.affiliation: Department of Surgical Neurology, Jichi Medical School, Minamikawachi-machi, Tochigi 329-0498, Japan. tmiyawaki@jichi.ac.jp
authors.forename: Takahiro
authors.initials: T
authors.lastname: Miyawaki
authors.forename: Hidenori
authors.initials: H
authors.lastname: Yokota
authors.forename: Keiji
authors.initials: K
authors.lastname: Oguro
authors.forename: Kengo
authors.initials: K
authors.lastname: Kato
authors.forename: Kuniko
authors.initials: K
authors.lastname: Shimazaki
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D003952:Diagnostic Imaging / Q000379:methods; D005110:Extracellular Space / Q000378:metabolism; D005849:Gerbillinae; D005947:Glucose / Q000172:deficiency*; D006624:Hippocampus / Q000166:cytology*; D000860:Hypoxia / Q000378:metabolism*; D066298:In Vitro Techniques; D007511:Ischemia / Q000378:metabolism*; D019194:Ischemic Preconditioning; D008297:Male; D009474:Neurons / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism*
chemical_list: D005947:Glucose; D015032:Zinc; D002118:Calcium