Selective adsorption of human CD4 T cells.
The pathogenesis of most autoimmune diseases directly involves CD4(+) helper T cells. To remove CD4(+) T cells selectively from the circulation, we designed a new column in which an anti-CD4 monoclonal antibody was immobilized on the activated substance. Nearly 90% of CD4(+) T cells were selectively adsorbed from whole blood with a single passage through the column in vitro, resulting in depletion of the antigen-specific T cell responses. We conclude that this new column would be potentially useful for treatment of T cell-mediated autoimmune diseases.
Matsuo
Hidenori
H
Division of Clinical Research, Kawatana National Hospital, Nagasaki, Japan. hidenori@kawatana.hosp.go.jp
Goto
Hirofumi
H
Kambara
Chiaki
C
Fukudome
Takayasu
T
Mizota
Takamitsu
T
Onodera
Hirokazu
H
Yoshida
Makoto
M
Shibuya
Noritoshi
N
D000911:Antibodies, Monoclonal / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002469:Cell Separation / Q000379:methods*; D016238:Cytapheresis / Q000379:methods*; D005434:Flow Cytometry; D006801:Humans
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens