title: Enniatins of Fusarium sp. strain F31 and their inhibition of Botrytis cinerea spore germination.
abstract: A spectrum of enniatins was isolated from Fusarium sp. strain F31 by bioassay-guided isolation directed against Botrytis cinerea. Two new enniatins, J(2) (7) and J(3) (8), were co-isolated and both contained, in addition to three hydroxyisovaleric acid units, N-methylated l-alanine, l-valine, and l-isoleucine units, differing only in their primary sequence. Two other enniatins, named enniatin J(1) (1) and enniatin K(1) (6), each containing two N-Me-l-Val units and one N-Me-l-Ala or alpha-N-Me-l-butyric acid unit, respectively, were isolated for the first time without directed biosynthesis. The enniatin structures were elucidated by spectroscopic and chemical methods, and the absolute configuration of the amino acids (l) and hydroxyisovaleric acid (d) was consistent with all previously isolated enniatins. The known enniatins B (2), B(1) (4), B(2) (5), and B(4) (3) were also isolated. The minimum inhibitory concentration of pure enniatins against Botrytis cinerea was 75 microg/mL.
authors.affiliation: Department of Chemistry, Swedish University of Agricultural Sciences, PO Box 7015, SE-750 07 Uppsala, Sweden. Anton.Pohanka@kemi.slu.se
authors.forename: Anton
authors.initials: A
authors.lastname: Pohanka
authors.forename: Kristof
authors.initials: K
authors.lastname: Capieau
authors.forename: Anders
authors.initials: A
authors.lastname: Broberg
authors.forename: Jan
authors.initials: J
authors.lastname: Stenlid
authors.forename: Elna
authors.initials: E
authors.lastname: Stenström
authors.forename: Lennart
authors.initials: L
authors.lastname: Kenne
mesh_terms: D000595:Amino Acid Sequence; D000613:Aminobutyrates / Q000138:chemical synthesis; D020171:Botrytis / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D005659:Fungicides, Industrial / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005670:Fusarium / Q000737:chemistry*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013172:Spores, Fungal / Q000187:drug effects*
chemical_list: D000613:Aminobutyrates; D005659:Fungicides, Industrial; D010456:Peptides, Cyclic; C487189:enniatin J2; C487190:enniatin, J3