title: [Screening of bioactive peptide that mimic the epitope of gastric cancer associated antigen].
abstract: OBJECTIVE
  To screen bioactive peptides that mimic the epitope of gastric cancer associated antigen.
  
  
  METHODS
  Anti-gastric cancer monoclonal antibody (mAb) MG7 was purified by ion chromatography, and then coated on ELISA plate. By using the mAb as selective molecular, a 12-meres phage displaying peptide library was biopanned, and the positive clones were selected.
  
  
  RESULTS
  12 positive phage clones were selected.
  
  
  CONCLUSIONS
  A candidate mimic epitope of gastric cancer associated antigen was identified, which provided the foundation for developing tumor peptide vaccine.
authors.affiliation: Department of Gastroenterology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Quan-Li
authors.initials: QL
authors.lastname: Hang
authors.forename: Jie
authors.initials: J
authors.lastname: Ding
authors.forename: Ai-Chun
authors.initials: AC
authors.lastname: Gong
authors.forename: Zhao-Cai
authors.initials: ZC
authors.lastname: Yu
authors.forename: Tai-Dong
authors.initials: TD
authors.lastname: Qiao
authors.forename: Bao-Jun
authors.initials: BJ
authors.lastname: Chen
authors.forename: Xue-Yong
authors.initials: XY
authors.lastname: Zhang
authors.forename: Dai-Ming
authors.initials: DM
authors.lastname: Fan
mesh_terms: D001435:Bacteriophages / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D006801:Humans; D019151:Peptide Library; D013274:Stomach Neoplasms / Q000276:immunology
chemical_list: D000939:Epitopes; D019151:Peptide Library