title: Activation of fibrinolysis and coagulation in non-occlusive intestinal ischaemia in a pig model.
abstract: The present study was carried out to explore the dynamics of the fibrinolytic and coagulation systems during non-occlusive intestinal ischaemia in a porcine model. Nineteen pigs underwent laparotomy. The inferior mesenteric artery and the collateral vessels to the rectum were ligated. The superior mesenteric artery (SMA) was isolated at its origin from the aorta, and constriction and flowmeter devices were applied. The blood flow of the SMA was reduced to cause ischaemia in the distal colon within the pHi interval 6.9-7.1. Fibrinogen, soluble fibrin, D-dimer, tissue plasminogen activator/plasminogen activator inhibitor-1 (tPA/PAI-1) complex and albumin were measured. Corrections for pig D-dimer and albumin were performed. Fibrinogen decreased significantly after laparotomy (P < 0.0001) and further after constriction of the SMA (P < 0.05), whereas soluble fibrin increased significantly (P < 0.005) after constriction of the SMA. The tPA/PAI-1 complex increased significantly after laparotomy (P < 0.05) and, after constriction of the SMA, the values first tended to decrease (P = 0.06) and then to increase (P = 0.056). The need to calibrate assays of human plasma proteins when applying them to experimental pig models was demonstrated. After constriction of the SMA, there was a rapidly reversed peak in the coagulation marker soluble fibrin and a strong tendency of alteration of the fibrinolytic marker tPA/PAI-1 complex.
authors.affiliation: Department of Surgery, Blekinge County Hospital, Orebro University Hospital, Sweden. stefan.acosta@telia.com
authors.forename: Stefan
authors.initials: S
authors.lastname: Acosta
authors.forename: Torbjörn K
authors.initials: TK
authors.lastname: Nilsson
authors.forename: David
authors.initials: D
authors.lastname: Bergqvist
authors.forename: Martin
authors.initials: M
authors.lastname: Björck
mesh_terms: D000818:Animals; D001777:Blood Coagulation; D017091:Colitis, Ischemic / Q000097:blood*; D003106:Colon / Q000098:blood supply / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis; D005342:Fibrinolysis; D008297:Male; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood; D012709:Serum Albumin / Q000032:analysis; D013552:Swine; D010959:Tissue Plasminogen Activator / Q000097:blood
chemical_list: D005338:Fibrin Fibrinogen Degradation Products; D017395:Plasminogen Activator Inhibitor 1; D012709:Serum Albumin; C036309:fibrin fragment D; D010959:Tissue Plasminogen Activator