Evaluation of bronchoalveolar lavage fluid cytotoxic parameters after inhalation exposure to amosite and wollastonite fibrous dusts combined with cigarette smoke.
The aim of this work was to compare the influence of amosite-asbestos and wollastonite fibrous dusts combined with cigarette smoke on chosen cytotoxic parameters of bronchoalveolar lavage fluid (BALF) in rats. Fisher 344 rats inhaled wollastonite or amosite fibrous dusts (60 or 30 mg x m(-3) air) one hour every two days combined with daily breathing of diluted mainstream tobacco smoke (30 mg of TPM x m(-3) air). The experiment lasted 6 months. After sacrifying the animals bronchoalveolar lavage (BAL) was performed and the viability and phagocytic activity of alveolar macrophages (AM), lactate dehydrogenase (LDH) and alkaline phosphatase activity (in the cell-free BALF), acid phosphatase (ACP) and cathepsin D activity (in cell-free BALF and BAL cell suspension) were examined. Exposure to amosite without tobacco smoke significantly decreased the viability of AM and increased the cathepsin D activity in BAL cells. Exposure to wollastonite significantly increased only the cathepsin D activity in BAL cells. Smoking significantly depressed the phagocytic activity of AM and amplified the amosite-induced increase of lysosomal enzyme activities--especially the activity of cathepsin D in BAL cells.
Cerná
S
S
Slovak Medical University, Institute of Preventive and Clinical Medicine, Bratislava, Slovak Republic. cerna@upkm.sk
Beno
M
M
Hurbánková
M
M
Kováciková
Z
Z
Bobek
P
P
Kyrtopoulos
S A
SA
D000818:Animals; D017639:Asbestos, Amosite / Q000633:toxicity*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry* / Q000166:cytology; D017610:Calcium Compounds / Q000633:toxicity*; D004391:Dust; D019570:Inhalation Exposure; D016676:Macrophages, Alveolar / Q000737:chemistry / Q000201:enzymology; D051381:Rats; D011916:Rats, Inbred F344; D017640:Silicates / Q000633:toxicity*; D012907:Smoking / Q000009:adverse effects*; D018709:Statistics, Nonparametric
D017610:Calcium Compounds; D004391:Dust; D017640:Silicates; D017639:Asbestos, Amosite; C031293:calcium silicate