title: Identifying gp85-regions involved in Epstein-Barr virus binding to B-lymphocytes.
abstract: Epstein-Barr virus lacking glycoprotein gp85 cannot infect B-cells and epithelial cells. The gp85 belongs to the molecular complex required for virus invasion of B-lymphocyte or epithelial cells. Moreover, there is evidence that gp85 is necessary for virus attachment to epithelial cells. Thirty-six peptides from the entire gp85-sequence were tested in epithelial and lymphoblastoid cell line binding assays to identify gp85-regions involved in virus-cell interaction. Five of these peptides presented high binding activity to Raji, Ramos, P3HR-1, and HeLa cells, but not to erythrocytes; Raji-cell affinity constants were between 80 and 140nM. Of these five peptides, 11435 ((181)TYKRVTEKGDEHVLSLVFGK(200)), 11436 ((201)TKDLPDLRGPFSYPSLTSAQ(220)), and 11438 ((241)YFVPNLKDMFSRAVTMTAAS(260)) bound to a 65kDa protein on Raji-cell surface. These peptides and antibodies induced by them (recognising live EBV-infected cells) inhibited Epstein-Barr virus interaction with cord blood lymphocytes. It is thus probable that gp85-regions defined by peptides 11435, 11436, and 11438 are involved in EBV invasion of B-lymphocytes.
authors.affiliation: Fundación Instituto de Inmunología de Colombia and Universidad Nacional de Colombia, Bogota, Colombia.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Urquiza
authors.forename: Jorge
authors.initials: J
authors.lastname: Suarez
authors.forename: Ramses
authors.initials: R
authors.lastname: Lopez
authors.forename: Erika
authors.initials: E
authors.lastname: Vega
authors.forename: Helena
authors.initials: H
authors.lastname: Patino
authors.forename: Javier
authors.initials: J
authors.lastname: Garcia
authors.forename: Manuel A
authors.initials: MA
authors.lastname: Patarroyo
authors.forename: Fanny
authors.initials: F
authors.lastname: Guzman
authors.forename: Manuel E
authors.initials: ME
authors.lastname: Patarroyo
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000821:virology*; D015153:Blotting, Western; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002469:Cell Separation; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000821:virology; D005434:Flow Cytometry; D006367:HeLa Cells; D004854:Herpesvirus 4, Human / Q000378:metabolism*; D006801:Humans; D007136:Immunoglobulins / Q000737:chemistry; D007962:Leukocytes / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D014759:Viral Envelope Proteins / Q000737:chemistry*
chemical_list: D007136:Immunoglobulins; D010455:Peptides; D014759:Viral Envelope Proteins; C090195:glycoprotein 85, Epstein-Barr virus