Transfection of the human malaria parasite Plasmodium falciparum.
Methods to transiently and stably transfect blood stages of the human malaria parasite Plasmodium falciparum have been developed and adapted for gene-knockout, allelic replacement, and transgene expression in this organism. These methods are detailed in this chapter, as are approaches used to monitor transfectants during the selection process. The different plasmid vectors that are currently used for gene targeting and transgene expression (including green fluorescent protein expression) are also described.
Crabb
Brendan S
BS
Division of Infection and Immunity, The Walter and Eliza Hall Institute of Medical Research, Victoria, Australia.
Rug
Melanie
M
Gilberger
Tim-Wolf
TW
Thompson
Jennifer K
JK
Triglia
Tony
T
Maier
Alexander G
AG
Cowman
Alan F
AF
D000483:Alleles; D000818:Animals; D010957:Plasmids; D010963:Plasmodium falciparum / Q000235:genetics*; D014162:Transfection; D019076:Transgenes