Application of DNA chip techniques for Yq microdeletion analysis in infertile males.
Our aim was to apply DNA chip technology as a diagnostic tool in infertility research and clinics. Six loci, including a sex-determining region on the Y chromosome and five sequence-tagged sites in azoospermia-factor regions were investigated in infertile male patients. Our method produced a sensitive signal, which showed the presence or absence of the STS regions on the Y chromosome. The results from 93 patients with non- obstructive azoospermia, oligoathenoteratozoospermia, or oligozoospermia were identical when analyzed with either the DNA chip technique or conventional PCR-gel electrophoresis. We have demonstrated its application in the molecular diagnosis of male infertility. This system provides an economic and high-throughput method for detecting the deletion of genomic DNA sequences of large groups of infertile patients, and a completely new approach to male infertility screening. The application of DNA chip technology to identify Yq deletions can also facilitate our understanding of male infertility.
Lee
Suman
S
Genome Research Center for Reproductive Medicine and Infertility, Infertility Medical Center, CHA General Hospital College of Medicine, Pochon CHA University, Seoul 135-913, Korea.
Joo
Hyun Suk
HS
Lee
Sook-Hwan
SH
Park
Jung Eun
JE
Kim
Jong Man
JM
Hwang
Jae Hoon
JH
Cho
Key Seung
KS
Hwang
Seung Yong
SY
D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods; D004587:Electrophoresis, Agar Gel; D005260:Female; D056426:Genetic Loci; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis* / Q000235:genetics*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D029589:Seminal Plasma Proteins / Q000235:genetics*; D012680:Sensitivity and Specificity; D016324:Sequence Tagged Sites; D012729:Sex Chromosome Aberrations
D029589:Seminal Plasma Proteins