Localization of infection-related epitopes on the non-structural protein 3ABC of foot-and-mouth disease virus and the application of tandem epitopes.
By means of overlapping peptides expressed in Escherichia coli in combination with Western-blotting, infection specific linear epitopes were identified on the non-structural protein 3ABC of FMDV. The epitopes reacted with sera from pigs or guinea pigs infected with different serotypes of FMDV, but not with sera from normal or vaccinated animals. A protein was constructed by tandem repeat of the epitope covering amino acid residues 141-190 on 3ABC. An ELISA based on the protein with tandem epitopes could be used as a diagnostic antigen for differentiating infected pigs from vaccinated ones.
Sun
Tao
T
Biotechnology Institute, School of Agriculture and Biology, Shanghai JiaoTong University, 2678 Qixin Road, Shanghai 201101, PR China.
Lu
Ping
P
Wang
Xin
X
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D005536:Foot-and-Mouth Disease / Q000175:diagnosis* / Q000276:immunology / Q000517:prevention & control; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000276:immunology; D013552:Swine; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines