Suppression subtractive hybridization-mediated transcriptome analysis from multiple tissues of aspen (Populus tremuloides) altered in phenylpropanoid metabolism.
A PCR-based suppression subtractive hybridization (SSH) technique was used to identify differentially expressed genes in developing tissues of control and transgenic aspen (Populus tremuloides Michx.) with down-regulated 4CL1 (4-coumarate:coenzyme A ligase) expression and enhanced growth. A total of 11,308 expressed sequence tags (ESTs) representing 5,028 non-redundant transcripts encoding 4,224 unique proteins was obtained from shoot apex, young stem, young leaf and root tip SSH libraries. Putative functions can be assigned to 60% of these transcripts. Approximately 14% of the ESTs are not represented among the 111,000 entries already present in Populus EST databases. In general, ESTs of the metabolism class occurred at a higher frequency in control- than transgenic-enriched libraries of all tissues, whereas protein synthesis and protein fate ESTs were over-represented in meristematic tissues of transgenics where 4CL1 was relatively strongly suppressed. Among all tissues, leaves yielded the highest percentage of ESTs with either unknown protein function or insignificant similarity to other protein/DNA/EST sequences in existing databases. Of particular interest was a large number of ESTs (16%) associated with signal transduction in transgenic leaves. Among these were several leucine-rich-repeat receptor-like protein kinases with markedly elevated expression in transgenic leaves. We also identified homologs of transposable elements that were up-regulated in transgenic tissues, providing the first experimental data for active expression of DNA mobile elements in long-lived tree species.
Ranjan
Priya
P
Plant Biotechnology Research Center, School of Forest Resources and Environmental Science, Michigan Technological University, 1400 Townsend Drive, Houghton 49931, USA.
Kao
Yu-Ying
YY
Jiang
Hongying
H
Joshi
Chandrashekhar P
CP
Harding
Scott A
SA
Tsai
Chung-Jui
CJ
D004251:DNA Transposable Elements; D030562:Databases, Protein; D015536:Down-Regulation; D020224:Expressed Sequence Tags; D015870:Gene Expression; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D006824:Hybridization, Genetic; D010666:Phenylpropionates / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D032107:Populus / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D004251:DNA Transposable Elements; D010666:Phenylpropionates; C019521:phenylpropiolic acid