Solution structure of hypothetical Nudix hydrolase DR0079 from extremely radiation-resistant Deinococcus radiodurans bacterium.
Using nuclear magnetic resonance (NMR) based methods, including residual dipolar coupling restraints, we have determined the solution structure of the hypothetical Deinococcus radiodurans Nudix protein DR0079 (171 residues, MW = 19.3 kDa). The protein contains eight beta-strands and three alpha-helices organized into three subdomains: an N-terminal beta-sheet (1-34), a central Nudix core (35-140), and a C-terminal helix-turn-helix (141-171). The Nudix core and the C-terminal helix-turn-helix form the fundamental fold common to the Nudix family, a large mixed beta-sheet sandwiched between alpha-helices. The residues that compose the signature Nudix sequence, GX5EX7REUXEEXGU (where U = I, L, or V and X = any amino acid), are contained in a turn-helix-turn motif on the face of the mixed beta-sheet. Chemical shift mapping experiments suggest that DR0079 binds Mg2+. Experiments designed to determine the biological function of the protein indicate that it is not a type I isopentenyl-diphosphate delta-isomerase and that it does not bind alpha,beta-methyleneadenosine 5'-triphosphate (AMPCPP) or guanosine 5'-[beta,gamma-imido]triphosphate (GMPPNP). In this article, the structure of DR0079 is compared to other known Nudix protein structures, a potential substrate-binding surface is proposed, and its possible biological function is discussed.
Buchko
Garry W
GW
Fundamental Sciences, Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, USA.
Ni
Shuisong
S
Holbrook
Stephen R
SR
Kennedy
Michael A
MA
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001665:Binding Sites; D034301:Deinococcus / Q000201:enzymology* / Q000502:physiology; D008274:Magnesium / Q000378:metabolism / Q000494:pharmacology; D008345:Manganese / Q000378:metabolism / Q000494:pharmacology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary / Q000187:drug effects; D011755:Pyrophosphatases / Q000737:chemistry* / Q000378:metabolism; D011836:Radiation Tolerance; D016415:Sequence Alignment; D012996:Solutions; D013329:Structure-Activity Relationship; D000096964:Nudix Hydrolases
D008274:Magnesium; D008345:Manganese; D011755:Pyrophosphatases; D012996:Solutions; D000096964:Nudix Hydrolases