title: Cleavage inhibition of the murine coronavirus spike protein by a furin-like enzyme affects cell-cell but not virus-cell fusion.
abstract: Cleavage of the mouse hepatitis coronavirus strain A59 spike protein was blocked in a concentration-dependent manner by a peptide furin inhibitor, indicating that furin or a furin-like enzyme is responsible for this process. While cell-cell fusion was clearly affected by preventing spike protein cleavage, virus-cell fusion was not, indicating that these events have different requirements.
authors.affiliation: Virology Division, Department of Infectious Diseases & Immunology, Yalelaan 1, 3584CL Utrecht, The Netherlands. p.rottier@vet.uu.nl
authors.forename: Cornelis A M
authors.initials: CA
authors.lastname: de Haan
authors.forename: Konrad
authors.initials: K
authors.lastname: Stadler
authors.forename: Gert-Jan
authors.initials: GJ
authors.lastname: Godeke
authors.forename: Berend Jan
authors.initials: BJ
authors.lastname: Bosch
authors.forename: Peter J M
authors.initials: PJ
authors.lastname: Rottier
mesh_terms: D004705:Endocytosis; D045683:Furin / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008561:Membrane Fusion; D008562:Membrane Glycoproteins / Q000378:metabolism*; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000378:metabolism*
chemical_list: D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; D045683:Furin