[Preparation of monoclonal antibodies against morphine and preliminary application of its colloidal gold conjugates].
OBJECTIVE
To prepare monoclonal antibodies (mAbs) against morphine.


METHODS
Morphine was conjugated to rabbit serum albumin or bovine serum albumin, respectively and the BALB/c mice were immunized with the conjugated. The mAbs against morphine were obtained by hybridoma technique. The urine samples were detected with the paper chromatography based on crosslinked of the purified mAb with colloidal gold.


RESULTS
Three mAbs against morphine were obtained and the sensitivity detected by paper chromatography reached to 200 microg/L.


CONCLUSIONS
The successful preparation of the mAb and its colloid gold conjugates may be useful for field check.
Xi
Xin
X
Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.
Jin
Bo-Quan
BQ
Zhu
Yong
Y
Liu
Xue-Song
XS
Wang
Wei-Xue
WX
Li
Xiao-Song
XS
Wu
Han-Yuan
HY
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D017979:Gold Colloid; D006825:Hybridomas / Q000276:immunology; D008807:Mice, Inbred BALB C; D009020:Morphine
D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D009020:Morphine