title: Evaluation of serum values of pancreatic enzymes after endoscopic retrograde pancreatography in dogs.
abstract: OBJECTIVE
  To assess the safety of endoscopic retrograde pancreatography (ERP) in dogs by performing repeated clinical examinations and laboratory analyses of serum amylase, lipase, canine trypsin-like immunoreactivity (cTLI), and canine pancreatic elastase 1 (cE1) after the procedure.
  
  
  METHODS
  7 healthy Beagles.
  
  
  METHODS
  Clinical examinations were performed and blood samples obtained for serum enzyme determinations before and at intervals (10 minutes; 2, 4, and 6 hours; and 1, 2, and 3 days) after ERP.
  
  
  RESULTS
  Repeated clinical examinations revealed no signs of ERP-induced complications in the 7 dogs. Results of repeated laboratory tests indicated a transient increase in serum values of amylase, lipase, and cTLI but not cE1. Mean +/- SD lipase activity increased from 120.7 +/- 116.4 U/L to 423.4 +/- 243.1 U/L at 4 hours after ERP. Median serum cTLI concentration increased from 16.2 microg/L (range, 77 to 26.5 microg/L) to 34.9 microg/L (range, 16.6 to 68.3 microg/L) 10 minutes after ERP. Enzyme values returned to baseline levels at the latest on day 2 in 6 of 7 dogs. Highest values for serum amylase, lipase, and cTLI and their delayed return to baseline values were detected in 1 dog with contrast filling of the pancreatic parenchyma.
  
  
  CONCLUSIONS
  Results indicated that ERP appears to be a safe imaging technique of pancreatic ducts in healthy dogs, although it induced a transient increase in serum values of pancreatic enzymes. In dogs, repeated clinical examinations and serum enzyme determinations can be used to monitor ERP-induced complications such as acute pancreatitis.
authors.affiliation: Department of Small Animal Internal Medicine at the Medical and Forensic Veterinary Clinic I, Justus-Liebig-University Giessen, Frankfurter Strasse 126, 35392 Giessen, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Spillmann
authors.forename: Irmeli
authors.initials: I
authors.lastname: Happonen
authors.forename: Satu
authors.initials: S
authors.lastname: Sankari
authors.forename: Andrea
authors.initials: A
authors.lastname: Wittker
authors.forename: Tuomo
authors.initials: T
authors.lastname: Kähkönen
authors.forename: Elias
authors.initials: E
authors.lastname: Westermarck
mesh_terms: D000681:Amylases / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D002760:Cholangiopancreatography, Endoscopic Retrograde / Q000009:adverse effects* / Q000379:methods; D004285:Dogs / Q000097:blood*; D008049:Lipase / Q000097:blood; D010179:Pancreas / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000097:blood; D014357:Trypsin / Q000097:blood / Q000276:immunology
chemical_list: D008049:Lipase; D000681:Amylases; D010196:Pancreatic Elastase; D014357:Trypsin