title: Role of VLDL receptor in the process of foam cell formation.
abstract: The role of very low density lipoprotein receptor (LVLDR) in the process of foam cell formation was investigated. After the primary cultured mouse peritoneal macrophages were incubated with VLDL, beta-VLDL or low density lipoprotein (LDL), respectively for 24 h and 48 h, foam cells formation was identified by oil red O staining and cellular contents of triglyceride (TG) and total cholesterol (TC) were determined. The mRNA levels of LDLR, LDLR related protein (ILRP) and VLDLR were detected by semi-quantitative RT-PCR. The results demonstrated that VLDL, beta-VLDL and LDL could increase the contents of TG and TC in macrophages. Cells treated with VLDL or beta-VLDL showed markedly increased expression of VLDLR and decreased expression of LDLR, whereas LRP was up-regulated slightly. For identifying the effect of VLDL receptor on cellular lipid accumulation, ldl-A7-VR cells, which expresses VLDLR and trace amount of LRP without functional LDLR, was used to incubate with lipoproteins for further examination. The results elucidated that the uptake of triglyceride-rich lipoprotein mediated by VLDLR plays an important role in accumulation of lipid and the formation of foam cells.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
authors.forename: Shen
authors.initials: S
authors.lastname: Qu
authors.forename: Fan
authors.initials: F
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Tian
authors.forename: Yinghong
authors.initials: Y
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yuzhe
authors.initials: Y
authors.lastname: Wang
authors.forename: Yiqiang
authors.initials: Y
authors.lastname: Zong
mesh_terms: D000818:Animals; D001161:Arteriosclerosis / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D008078:Cholesterol, LDL / Q000378:metabolism / Q000494:pharmacology; D005260:Female; D005487:Foam Cells / Q000166:cytology* / Q000378:metabolism; D008079:Lipoproteins, VLDL / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D011973:Receptors, LDL / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D008078:Cholesterol, LDL; D008079:Lipoproteins, VLDL; D011973:Receptors, LDL; D014280:Triglycerides; C036053:VLDL receptor