The proximal 2-kb of the Hoxa3 promoter directs gene expression in distinct branchial compartments and cranial ganglia.
Developing structures such as hindbrain, neural crest cells or spinal cord express Hoxa3. Here, we have investigated the regulatory role of a 2-kb fragment spanning the proximal promoter of Hoxa3 by a reporter-based approach in mice. We show that this fragment promotes reporter activity in ganglionic and branchial compartments known to express Hoxa3 but for which no cis-regulatory elements have been identified so far. We also show that the 2-kb promoter fragment is active in rhombomere 4 and in the ganglion of the cranial nerve complex VII/VIII that are devoid of Hoxa3 expression.
Diman
Nata Y S-G
NY
Unit of Developmental Genetics, Université Catholique de Louvain, 73 (box 82) Avenue Mounier, Brussels 1200, Belgium.
Chauvier
Ethel
E
Pacico
Nathalie
N
Picard
Jacques J
JJ
Rezsohazy
René
R
D000818:Animals; D001934:Branchial Region / Q000196:embryology / Q000378:metabolism*; D003391:Cranial Nerves / Q000196:embryology / Q000378:metabolism*; D004622:Embryo, Mammalian; D004627:Embryonic Induction; D005725:Ganglia, Autonomic / Q000196:embryology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012249:Rhombencephalon / Q000196:embryology / Q000378:metabolism*
D018398:Homeodomain Proteins; C080331:Hoxa3 protein, mouse