title: Alteration of urinary sorbitol excretion in WBN-kob diabetic rats - treatment with an aldose reductase inhibitor.
abstract: An accelerated polyol pathway in diabetes contributes to the development of diabetic complications. To elucidate diabetic nephropathy involving also renal tubular damage, we measured urinary sorbitol concentration concomitantly with urinary N-acetyl-D-glucosaminidase (NAG) excretion in WBN-kob diabetic rats.Twenty-four-hour urinary sorbitol concentrations increased in the diabetic rats in parallel with whole blood sorbitol concentrations. An increase in 24-h urinary NAG excretion coincided with the elevated urinary sorbitol levels in the diabetic rats. The administration of epalrestat, an aldose reductase inhibitor, reduced the increased whole blood and urinary sorbitol concentrations and urinary NAG excretion concomitantly with renal aldose reductase inhibition in the diabetic rats. These results indicate that diabetic nephropathy involves distorted cell function of renal tubules, and that treatment with epalrestat may prevent at least the progress of the nephropathy.
authors.affiliation: Department of Internal Medicine, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Tsugawa
authors.forename: Rikio
authors.initials: R
authors.lastname: Shinohara
authors.forename: Akio
authors.initials: A
authors.lastname: Nagasaka
authors.forename: Itsuko
authors.initials: I
authors.lastname: Nakano
authors.forename: Fumiko
authors.initials: F
authors.lastname: Takeda
authors.forename: Minoru
authors.initials: M
authors.lastname: Nagata
authors.forename: Naohisa
authors.initials: N
authors.lastname: Oda
authors.forename: Yoshikuni
authors.initials: Y
authors.lastname: Sawai
authors.forename: Nobuki
authors.initials: N
authors.lastname: Hayakawa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Suzuki
authors.forename: Mitsuyasu
authors.initials: M
authors.lastname: Itoh
mesh_terms: D000118:Acetylglucosaminidase / Q000652:urine; D000449:Aldehyde Reductase / Q000032:analysis / Q000037:antagonists & inhibitors; D000818:Animals; D003928:Diabetic Nephropathies / Q000188:drug therapy / Q000378:metabolism / Q000652:urine*; D007668:Kidney / Q000378:metabolism; D008297:Male; D023421:Models, Animal; D051381:Rats; D017208:Rats, Wistar; D012236:Rhodanine / Q000031:analogs & derivatives* / Q000627:therapeutic use*; D013012:Sorbitol / Q000652:urine*; D053778:Thiazolidines
chemical_list: D053778:Thiazolidines; C038131:epalrestat; D013012:Sorbitol; D012236:Rhodanine; D000449:Aldehyde Reductase; D000118:Acetylglucosaminidase