Ribosomal crystallography: a flexible nucleotide anchoring tRNA translocation, facilitates peptide-bond formation, chirality discrimination and antibiotics synergism.
The linkage between internal ribosomal symmetry and transfer RNA (tRNA) positioning confirmed positional catalysis of amino-acid polymerization. Peptide bonds are formed concurrently with tRNA-3' end rotatory motion, in conjunction with the overall messenger RNA (mRNA)/tRNA translocation. Accurate substrate alignment, mandatory for the processivity of protein biosynthesis, is governed by remote interactions. Inherent flexibility of a conserved nucleotide, anchoring the rotatory motion, facilitates chirality discrimination and antibiotics synergism. Potential tRNA interactions explain the universality of the tRNA CCA-end and P-site preference of initial tRNA. The interactions of protein L2 tail with the symmetry-related region periphery explain its conservation and its contributions to nascent chain elongation.
Agmon
Ilana
I
Department of Structural Biology, The Weizmann Institute, 76100 Rehovot, Israel.
Amit
Maya
M
Auerbach
Tamar
T
Bashan
Anat
A
Baram
David
D
Bartels
Heike
H
Berisio
Rita
R
Greenberg
Inbal
I
Harms
Joerg
J
Hansen
Harly A S
HA
Kessler
Maggie
M
Pyetan
Erez
E
Schluenzen
Frank
F
Sittner
Assa
A
Yonath
Ada
A
Zarivach
Raz
R
D000596:Amino Acids / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry; D017963:Azithromycin / Q000494:pharmacology; D002384:Catalysis; D018360:Crystallography, X-Ray / Q000379:methods*; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry; D020033:Protein Isoforms; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D012333:RNA, Messenger / Q000378:metabolism; D012343:RNA, Transfer / Q000737:chemistry / Q000378:metabolism; D012270:Ribosomes / Q000737:chemistry* / Q000648:ultrastructure*; D013379:Substrate Specificity; D014769:Virginiamycin / Q000494:pharmacology
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D010455:Peptides; D020033:Protein Isoforms; D012333:RNA, Messenger; D014769:Virginiamycin; C062940:quinupristin-dalfopristin; D017963:Azithromycin; D012343:RNA, Transfer