Purification of goat immunoglobulin G by immobilized metal-ion affinity using cross-linked alginate beads.
The alginate beads obtained by cross-linking of the polymer by epichlorohydrin were charged with Cu(II). The copper-charged beads could be directly used as a immobilized-metal-affinity-chromatographic medium for purification of goat IgG. The best results in the packed-bed mode were obtained by using beads charged with 95.4 micromol/ml Cu(II). We found that we could recover 97.4% IgG with an 8-fold purification.
Jain
Sulakshana
S
Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India.
Gupta
Munishwar N
MN
D000464:Alginates / Q000737:chemistry*; D000818:Animals; D002846:Chromatography, Affinity; D003300:Copper / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D004811:Epichlorohydrin / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry*; D006041:Goats; D006603:Hexuronic Acids / Q000737:chemistry*; D007074:Immunoglobulin G / Q000097:blood / Q000302:isolation & purification*; D008670:Metals / Q000737:chemistry*; D008863:Microspheres
D000464:Alginates; D003432:Cross-Linking Reagents; D006603:Hexuronic Acids; D007074:Immunoglobulin G; D008670:Metals; D004811:Epichlorohydrin; D003300:Copper; D020723:Glucuronic Acid