Spinning-disk self-referencing interferometry of antigen-antibody recognition.
A gold ridge microstructure fabricated to a height of lambda/8 on a high-reflectivity substrate behaves as a wave-front-splitting self-referencing interferometer in phase quadrature when illuminated by a Gaussian laser beam and observed in the far field along the optic axis. When immuno-gammaglobulin (IgG) antibodies are selectively immobilized on the gold microstructure, they recognize and bind to a specific antigen, which shifts the relative optical phase of the interferometer and modifies the far-field diffracted intensity. We detect bound antigen interferometrically on spinning disks at a sampling rate of 100 kHz and verify the interferometric nature of the signal by using two quadratures of opposite sign to rule out effects of dynamic light scattering. Strong molecular recognition is demonstrated by the absence of binding to nontarget molecules but strong signal change in response to a specific antigen. This BioCD has the potential to be applied as a spinning-disk interferometric immunoassay and biosensor.
Varma
M M
MM
Department of Physics, Purdue University, 525 Northwestern Avenue, West Lafayette, Indiana 47907, USA. manoj@physics.purdue.edu
Nolte
D D
DD
Inerowicz
H D
HD
Regnier
F E
FE
D000818:Animals; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000276:immunology; D016250:Compact Disks; D007074:Immunoglobulin G / Q000276:immunology; D007368:Interferometry / Q000295:instrumentation* / Q000379:methods*; D007834:Lasers
D000941:Antigens; D007074:Immunoglobulin G