title: Activation of phospholipase D by osmotic cell swelling.
abstract: In response to osmotic cell swelling, Intestine 407 cells react with a rapid and transient activation of phospholipase D (PLD). To investigate the role of PLD during the regulatory volume decrease, cells were treated with 1-butanol resulting in a depletion of PLD substrates. Activation of volume-regulated anion channels, but not the cell swelling-induced release of taurine, was largely inhibited in the presence of low concentrations of 1-butanol. In addition, hypotonicity-induced exocytosis, ATP release and subsequent endocytosis were found to be largely abrogated. The results support a model of cell volume regulation in which PLD plays an essential role in the cell swelling-induced vesicle cycling and in the activation of volume-sensitive anion channels.
authors.affiliation: Department of Biochemistry, Erasmus University Medical Center, P.O. Box 1738, 3000DR Rotterdam, The Netherlands.
authors.forename: Sebastian F B
authors.initials: SF
authors.lastname: Tomassen
authors.forename: Thea
authors.initials: T
authors.lastname: van der Wijk
authors.forename: Hugo R
authors.initials: HR
authors.lastname: de Jonge
authors.forename: Ben C
authors.initials: BC
authors.lastname: Tilly
mesh_terms: D020001:1-Butanol / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism; D002460:Cell Line; D048429:Cell Size / Q000187:drug effects / Q000502:physiology*; D003911:Dextrans / Q000737:chemistry / Q000378:metabolism; D004789:Enzyme Activation; D006801:Humans; D007038:Hypotonic Solutions / Q000494:pharmacology; D007422:Intestines / Q000166:cytology; D008856:Microscopy, Fluorescence; D009997:Osmotic Pressure; D010739:Phospholipase D / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D011868:Radioisotopes; D013654:Taurine / Q000378:metabolism
chemical_list: D003911:Dextrans; D007038:Hypotonic Solutions; D010743:Phospholipids; D011868:Radioisotopes; D013654:Taurine; D000255:Adenosine Triphosphate; D020001:1-Butanol; D010739:Phospholipase D