Monosialoganglioside protected ischemic rat hippocampal slices through stabilizing expression of N-methyl-D-aspartate receptor subunit.
OBJECTIVE
To determine direct protective effect of monosialoganglioside (GM1) on hippocampal slices after oxygen-glucose deprivation and reperfusion (OGD/RP), and investigate the influence on the expression of N-methyl-D-aspartate receptor subunit 1 (NMDAR1) in those hippocampal slices.


METHODS
Injury of hippocampal slices and protective effects of GM1 were detected by 2,3,5-triphenyltetrazolium chloride (TTC) staining, toluidine blue staining, and transmission electron microscopy of rat hippocampal slices. Expression of NMDAR1 was detected by Western blot.


RESULTS
(1) GM1 at 1.0 micromol/L was the most effective concentration to preserve the TTC staining of the hippocampal slices after OGD/RP (P<0.05), and the next was GM1 at 10.0 micromol/L (P<0.05). (2) Toluidine blue staining and transmission electron microscopy showed GM1 protected the injuried hippocamal slices after OGD/RP. (3) GM1 downregulated the temporally high expression of NMDAR1 in the hippocampal slices immediately after a 25-min OGD and prevented the over low expression of NMDAR1 after a 30-min reperfusion.


CONCLUSIONS
GM1 could protect injuried rat hippocampal slices after OGD/RP through stabilizing the expression of NMDAR1.
Liu
Jian-Ren
JR
Neurology Department, 2 nd Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China.
Ding
Mei-Ping
MP
Wei
Er-Qing
EQ
Huang
Jian-Zheng
JZ
Song
Ying
Y
Ding
Qiang
Q
Ge
Qiu-Fu
QF
D000818:Animals; D005677:G(M1) Ganglioside / Q000494:pharmacology*; D005947:Glucose / Q000172:deficiency*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D009474:Neurons / Q000378:metabolism; D010100:Oxygen / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*
D005677:G(M1) Ganglioside; D005947:Glucose; D010100:Oxygen; D016194:Receptors, N-Methyl-D-Aspartate