[Study on a new IL-12 inducing gene NAP2].
OBJECTIVE
To study the function of newly cloned NF-kappaB-activating gene (NAP2) and its mechanism. METHEDS: Activation of NF-kappaB and IL-12 promoters as well as modulation of IL-12 synthesis by NAP2 in macrophages were observed by NF-kappaB and IL-12 luciferase reporter assay and intracellular IL-12 staining.


RESULTS
NAP2 could activate NF-kappaB and IL-12 promoter. But IkappaBalphaDN could inhibit their activation. In addition, NAP2 could induce IL-12 expression in macrophage cell line J774.


CONCLUSIONS
NAP2 as a newly cloned NF-kappaB activating gene can activate IL-12 promoter and induce IL-12 expression in macrophages. The data suggest that NAP2 regulates the expression of IL-12 most likely through its modulation of NF-kappaB transcription factor.
Yang
Min
M
Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710032, China.
Liu
Na
N
Ting
Adrian T
AT
D002460:Cell Line; D005786:Gene Expression Regulation; D018664:Interleukin-12 / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D011401:Promoter Regions, Genetic
D016328:NF-kappa B; D018664:Interleukin-12