title: Presence of collagen IV in the ciliary zonules of the human eye: an immunohistochemical study by LM and TEM.
abstract: The ciliary zonules of the eye are composed of fibrillar and non-fibrillar components. Fibrils provide tensile strength and elasticity, whereas non-fibrillar components serve as a coating surrounding the fibrils. This coating behaves as a barrier to macromolecules. The present light and transmission electron microscopic (LM and TEM) study identified collagen IV as a novel component of this coating. Collagen IV was demonstrated by pre-embedding and postembedding immunohistochemical (IHC) techniques using monoclonal and polyclonal antibodies. The specificity of the polyclonal anticollagen IV antibody was verified by ELISA.
authors.affiliation: University Hospital/University of Groningen, Department of Ophthalmology, Groningen, The Netherlands. l.i.los@ohk.azg.nl
authors.forename: Leonoor I
authors.initials: LI
authors.lastname: Los
authors.forename: Roelofje J
authors.initials: RJ
authors.lastname: van der Worp
authors.forename: Marja J A
authors.initials: MJ
authors.lastname: van Luyn
authors.forename: Johanna M M
authors.initials: JM
authors.lastname: Hooymans
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D024141:Collagen Type IV / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005123:Eye / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D008853:Microscopy / Q000379:methods; D008875:Middle Aged
chemical_list: D024141:Collagen Type IV