title: Tissue-specific distribution of donor mitochondrial DNA in cloned mice produced by somatic cell nuclear transfer.
abstract: Highly diverse results have been reported for mitochondrial DNA (mtDNA) hetero-plasmy in nuclear-transferred farm animals. In this study, we cloned genetically defined mice and investigated donor mtDNA inheritance following somatic cell cloning. Polymerase chain reaction (PCR) analysis with primers that were specific for either the recipient oocytes or donor cells revealed that the donor mtDNA coexisted with the recipient mtDNA in the brain, liver, kidney, and tail tissues of 96% (24/25) of the adult clones. When the proportion of donor mtDNA in each tissue was measured by allele-specific quantitative PCR and subjected to ANOVA analysis, a tissue-specific mtDNA segregation pattern (P < 0.05) was observed, with the liver containing the highest proportion of donor mtDNA. Therefore, the donor mtDNA was inherited consistently by the cloned offspring, whereas donor mtDNA segregation was not neutral, which is in accordance with previous notions about tissue-specific nuclear control of mtDNA segregation.
authors.forename: Kimiko
authors.initials: K
authors.lastname: Inoue
authors.forename: Narumi
authors.initials: N
authors.lastname: Ogonuki
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Kaoru
authors.initials: K
authors.lastname: Takano
authors.forename: Hiromi
authors.initials: H
authors.lastname: Miki
authors.forename: Keiji
authors.initials: K
authors.lastname: Mochida
authors.forename: Atsuo
authors.initials: A
authors.lastname: Ogura
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002467:Cell Nucleus / Q000235:genetics; D020090:Chromosome Segregation; D019976:Cloning, Organism; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D005347:Fibroblasts / Q000502:physiology; D007668:Kidney / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008930:Mitochondria, Liver / Q000378:metabolism; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000502:physiology*; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D014019:Tissue Donors
chemical_list: D004272:DNA, Mitochondrial