title: A novel lectin with a fibrinogen-like domain and its potential involvement in the innate immune response of Armigeres subalbatus against bacteria.
abstract: Mosquitoes have an efficient cellular innate immune response that includes phagocytosis of microbial pathogens and encapsulation of metozoan parasites. In this study, we describe a novel lectin in the mosquito, Armigeres subalbatus (aslectin or AL-1). The 1.27 kb cDNA clone for the AL-1 gene (AL-1) encodes a 279 deduced amino acid sequence that contains a C-terminal fibrinogen-like domain. AL-1 is transcribed in all life stages. AL-1 mainly exists in the haemolymph of adult female mosquitoes, and is upregulated following both Escherichia coli and Micrococcus luteus challenge. AL-1 specifically recognizes N-acetyl-d-glucosamine and is able to bind both E. coli and M. luteus. These results suggest that AL-1 might function as a pattern recognition receptor in the immune response in Ar. subalbatus.
authors.affiliation: Department of Animal Health and Biomedical Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: T A
authors.initials: TA
authors.lastname: Rocheleau
authors.forename: J F
authors.initials: JF
authors.lastname: Fuchs
authors.forename: J F
authors.initials: JF
authors.lastname: Hillyer
authors.forename: C-C
authors.initials: CC
authors.lastname: Chen
authors.forename: B M
authors.initials: BM
authors.lastname: Christensen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D009033:Culicidae / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D040461:Gene Components; D005786:Gene Expression Regulation; D015723:Gene Library; D006458:Hemolymph / Q000378:metabolism; D007113:Immunity, Innate / Q000235:genetics*; D037102:Lectins / Q000235:genetics* / Q000378:metabolism*; D015336:Molecular Probe Techniques; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D037102:Lectins; D014157:Transcription Factors