[Construction and identification of recombinant retrovirus vector containg hTrx gene].
OBJECTIVE
To construct a recombinant retrovirus vector carrying hTrx gene.


METHODS
hTrx gene was cloned directionally into a retroviral vector pLXSN by DNA recombinant technique and the recombinant vector was identified EcoRI and BamHI digestion analysis. The recombinant was transfected into packaging cells PA317 via CaPO(4)-based transfection method, and viral titer in culture supernatant of transfected cells was detected.


RESULTS
The recombinant pLTrxSN had been constracted. EcoRI and BamHI digestion analysis displayed two positive bands of 0.3 kb and 5.9 kb respectively. The viral titer in supernatant of transfected cells was 5.5x10(9) cfu/L.


CONCLUSIONS
pLhTrxSN has been constructed successfully, which will be useful for Trx gene therapy and experiment study.
Li
Min-Jie
MJ
Neuroscience Center of Xi'an Jiaotong University Xi'an 710061, China.
Liu
Yong
Y
Zhao
Jian-Jun
JJ
Wang
Quan-Ying
QY
Yang
Guang-Xiao
GX
D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D012190:Retroviridae / Q000235:genetics; D014162:Transfection