[Simultaneous analysis of glyphosate and glufosinate in vegetables and fruits by GC-FPD].
A rapid analytical method for residues of the herbicide, glyphosate [N-(phosphonomethyl)glycine], glufosinate [DL-homoalanine-4-yl(methyl)phosphinic acid] and glufosinate metabolite (MPPA: 3-methylphosphinicopropionic acid) in vegetables and fruits was developed by improving the bulletin method of glufosinate. 50 mL of solution extracted with water (corresponding to 2 g of the sample) was loaded on a column packed with 5 mL of anion exchange resin and then the trapped glyphosate, glufosinate and MPPA were eluted with 60 mL of 50% acetic acid. After derivatization with trimethyl orthoacetate, the derivatives were purified and separated on a Florisil cartridge column. The determination of the derivatives was performed with GC-FPD. The detection limits for glyphosate, glufosinate and MPPA were 0.01 microgram/g, 0.01 microgram/g and 0.005 microgram/g, respectively. The recoveries from fortified samples were 83.5-89.8% for glyphosate, 77.9-92.2% for glufosinate and 75.0-87.2% for MPPA.
Watanabe
Sadao
S
Kanagawa Prefectural Insutitute of Public Health: 1-3-1, Shimomachiya, Chigasaki-shi 253-0087, Japan.
D000613:Aminobutyrates / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis*; D006540:Herbicides / Q000032:analysis*; D014675:Vegetables / Q000737:chemistry*; D000097797:Glyphosate
D000613:Aminobutyrates; D005998:Glycine; D006540:Herbicides; D000097797:Glyphosate; C003121:phosphinothricin