title: [Effects of leukemia bone marrow stromal cells on resistance of co-cultured HL-60 to idarubicin].
abstract: To study the role of hematopoietic microenvironment abnormality in development of minimal residual disease and its mechanism, the viability of HL-60 cells was investigated by means of bone marrow stromal cell culture system or co-culture system of bone marrow stromal cell with HL-60 cells and idarubicin (IDA), flow cytometry and ELISA. The results showed that viability of HL-60 cells gradually decreased along with the increase of IDA dose and prolongation of culture time. Amount of HL-60 cells co-cultured with leukemia bone marrow stramal cells was significantly increased as compared with that of the control (P < 0.05). Bone marrow stromal cells or stromal cell conditioned medium reduced the effect of IDA on HL-60 cells in culture. In conclusion, leukemia bone marrow stromal cells contribute to increasing resistance of HL-60 cells to chemotherapeutic agents, and play some role in developing minimal residual disease.
authors.affiliation: Department of Hematology, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Xian-Gui
authors.initials: XG
authors.lastname: Peng
authors.forename: Qing-Yu
authors.initials: QY
authors.lastname: Wang
authors.forename: Pei-Yan
authors.initials: PY
authors.lastname: Kong
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Gao
authors.forename: Yong-Ming
authors.initials: YM
authors.lastname: Zhong
mesh_terms: D001854:Bone Marrow Cells / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects; D018920:Coculture Techniques; D004305:Dose-Response Relationship, Drug; D019008:Drug Resistance, Neoplasm; D018922:HL-60 Cells / Q000187:drug effects*; D006801:Humans; D015255:Idarubicin / Q000494:pharmacology*; D017154:Stromal Cells / Q000502:physiology
chemical_list: D015255:Idarubicin