title: [Shistosoma japonicum: dynamics of cytokine production induced by immunization of mice with recombinant GST-Sj32 protein before and after challenge infection].
abstract: OBJECTIVE
  To explore the mechanism of protective immunity of rGST-Sj32.
  
  
  METHODS
  BALB/c mice were immunized subcutaneously with rGST-Sj32 emulsified with Freund's complete adjuvant. Five mice from each group were killed prior to immunization, prior to challenge and 10 days, 30 days and 45 days post-challenge, respectively. Spleen cells from these mice were cultured and levels of cytokine secreted by splenocytes were detected by ELISA.
  
  
  RESULTS
  Compared with the control group, the quantity of IL-4, IL-5 and IFN-gamma released from rGST-Sj32-primed splenocytes were increased to varying degrees, being (10.21+/-3.65) ng/L, (19.89+/-9.57) ng/L and (5.09+/-2.51) microg/L, respectively. The level of IFN-gamma post challenge did not increase in control group and immunization group. The levels of IL- 4 and IL-5 in the control group were elevated gradually following challenge, and were much higher than those in immunization group.
  
  
  CONCLUSIONS
  The immunization with rGST-Sj32 elicited mainly a Th1 type immune response in BALB/c mice, whereas the immune response induced by challenge was a Th2 type.
authors.affiliation: Department of Parasitology,Xiang-Ya School of Medicine, Central South University, Changsha 410078, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Cai
authors.forename: Xin-Yuan
authors.initials: XY
authors.lastname: Yi
authors.forename: Qing-Lin
authors.initials: QL
authors.lastname: Wang
authors.forename: Jin-Chun
authors.initials: JC
authors.lastname: Zhou
authors.forename: Xian-Fang
authors.initials: XF
authors.lastname: Zeng
authors.forename: Larry
authors.initials: L
authors.lastname: McReynolds
mesh_terms: D000818:Animals; D016207:Cytokines; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D014611:Vaccination
chemical_list: D016207:Cytokines; D011994:Recombinant Proteins