Model of small bowel transplantation with systemic venous drainage in rats.
Authors in details describe microsurgical technique of heterotopic small bowel transplantation (SBT) with systemic venous drainage in rats. Surgical technique was practiced on laboratory Wistar rat (n = 40), male with weight 240-300 g. This paper in details analyzes the surgical procedure and includes methods of anesthesia, organ harvest, methods of conservation, transplantation and their complications. The aim of authors is to describe a reproducible model of small bowel transplantation in rat and to get a basis for next experimental studies, which will investigate others aspects of SBT like preservation-reperfusion injury and immunological aspect as rejection. Five days of surviving of rats was accepted as successful.
Baláz
Peter
P
Transplant Surgery Department, Institute of Clinical and Experimental Medicine (IKEM), Prague 4, Czech Republic. peba@medicon.cz
Kudla
Michal
M
Matia
Ivan
I
Fronĕk
Jirí
J
Ryska
Miroslav
M
D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D013505:Digestive System Surgical Procedures / Q000379:methods; D007421:Intestine, Small / Q000098:blood supply / Q000637:transplantation*; D008297:Male; D008866:Microsurgery / Q000379:methods; D023421:Models, Animal; D051381:Rats; D017208:Rats, Wistar