Expression of prostaglandin D2 receptors DP1 and DP2 by human colorectal cancer cells.
The expression and function of prostaglandin (PG) D2 DP receptors during colorectal carcinogenesis has not been elucidated. Therefore, we studied expression of DP1 and DP2 receptors by reverse transcription-polymerase chain reaction analysis of receptor mRNA levels in five human colorectal cancer cell lines (HT-29, HCA-7, HCT116, SW480 and SW48) and VACO-235 human colorectal adenoma cells. DP1 receptor transcripts were present only in HT-29 cells. In addition, none of the human colorectal epithelial cell lines tested expressed DP2 receptor mRNA. Therefore, PGD2 is unlikely to have direct activity on neoplastic colorectal epithelial cells via cell surface DP receptors.
Hawcroft
Gillian
G
Molecular Medicine Unit, University of Leeds, Clinical Sciences Building, St James's University Hospital, Leeds LS9 7TF, UK.
Gardner
Sarah H
SH
Hull
Mark A
MA
D000236:Adenoma / Q000378:metabolism* / Q000473:pathology; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D017931:DNA Primers / Q000737:chemistry; D019073:HT29 Cells / Q000378:metabolism; D006801:Humans; D015230:Prostaglandin D2 / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D011971:Receptors, Immunologic; D011982:Receptors, Prostaglandin / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011971:Receptors, Immunologic; D011982:Receptors, Prostaglandin; D015230:Prostaglandin D2; C036293:prostaglandin D2 receptor