Marek's disease vaccination, with turkey herpesvirus, and enrofloxacin modulate the activities of hepatic microsomal enzymes in broiler chickens.
Chickens were vaccinated against Marek's disease intramuscularly at one day of age. Enrofloxacin was given ad libitum in the drinking water at concentrations of 50, 100 and 250 mg/L from 8 days to 13 days of age when the animals were killed and the activities of cytochrome P-450 enzymes in the liver were measured. Vaccinated non-treated chickens served as a positive control. A negative control group was neither vaccinated nor treated. Vaccination decreased the activity of aniline hydroxylase and ethylmorphine N-demethylase in the positive control group. Subsequent application of enrofloxacin in the lowest concentration (50 mg/L) decreased, while that given at the highest level (250 mg/L) significantly increased the activity of the same microsomal enzymes. Relative liver weights and concentrations of proteins in 9000 x g supernatant were not affected by vaccination or treatment.
Sakar
D
D
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia.
Prukner-Radovcić
Estela
E
Prevendar Crnić
Andreja
A
Pompe-Gotal
Jelena
J
Ragland
W L
WL
Mazija
H
H
D000815:Aniline Hydroxylase / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002645:Chickens; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000008:administration & dosage / Q000494:pharmacology*; D006564:Herpesviridae / Q000276:immunology*; D007273:Injections, Intramuscular / Q000662:veterinary; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008380:Marek Disease / Q000378:metabolism / Q000517:prevention & control*; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D015363:Quinolones / Q000008:administration & dosage / Q000494:pharmacology*; D014765:Viral Vaccines
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D015363:Quinolones; D014765:Viral Vaccines; D000077422:Enrofloxacin; D000815:Aniline Hydroxylase