[Immunochromatographic analysis of 2,4-dichlorophenoxyacetic acid and simazine using monoclonal antibodies labelled with colloidal gold].
A method of the competitive immunochromatographic assay of the pesticides 2,4-D (2,4-dichlorophenoxyacetic acid) and simazine (2-chloro-4,6-bis(N-ethylamino)-1,2,5-triazine) in aqueous samples was developed. Monoclonal antibodies to these pesticides labeled with colloidal gold were used to visualize the results. The sensitivity of the 2,4-D and simazine assay is 12 ng/ml, and the time of analysis is 3-7 min. The method does not differ in sensitivity from the competitive EIA using conjugates of monoclonal antibodies to the pesticides with horseradish peroxidase; however, the time of the EIA is 1.5 h. The immunochromatographic method of the pesticide detection is available and simple and may be recommended for the development of assays of any other low-molecular compounds. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 2; see also http://www.maik.ru.
Liubavina
I A
IA
Shemyakin-Ovchinnikov, Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP Moscow, 117997 Russia. lia@ibch.ru
Zinchenko
A A
AA
Salomatina
I S
IS
Zherdev
A V
AV
Dzantiev
B B
BB
D015084:2,4-Dichlorophenoxyacetic Acid / Q000032:analysis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002845:Chromatography / Q000379:methods*; D017979:Gold Colloid / Q000737:chemistry*; D007124:Immunoenzyme Techniques; D012680:Sensitivity and Specificity; D012839:Simazine / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D015084:2,4-Dichlorophenoxyacetic Acid; D012839:Simazine