A conserved non-homeodomain Hoxa9 isoform interacting with CBP is co-expressed with the 'typical' Hoxa9 protein during embryogenesis.
Various Hox genes are known to produce alternative transcripts encoding different isoforms whose physiological relevance during development is not yet understood. In this work, we analysed two different Hoxa9 mRNAs encoding a full-length protein (Hoxa9) or a protein lacking the homeodomain (Hoxa9T). First, we demonstrated that these transcripts are conserved from birds to mammals. We then showed that both transcripts are present throughout embryogenesis and that Hoxa9T transcript is particularly abundant in embryonic genital tract, kidney, forelimb and tail. We further found that both isoforms are able to interact with CBP, suggesting a competition between Hoxa9 and Hoxa9T with this protein.
Dintilhac
Agnès
A
UMR 6061, Génétique et Développement, IFR 97, Université de Rennes 1, Campus Villejean, 2 avenue du Professeur Léon Bernard, CS34317, F-35043 Rennes, France.
Bihan
Réjane
R
Guerrier
Daniel
D
Deschamps
Stéphane
S
Pellerin
Isabelle
I
D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D017930:Genes, Reporter; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA
D018398:Homeodomain Proteins; D008565:Membrane Proteins; C498709:Pag1 protein, mouse; D010750:Phosphoproteins; D020033:Protein Isoforms; D012333:RNA, Messenger; C097899:homeobox protein HOXA9