title: Expression of pro-inflammatory cytokines in rainbow trout (Oncorhynchus mykiss) during an infection with Ichthyophthirius multifiliis.
abstract: Inflammatory processes are known to take place in the skin of fish during an infection with the parasitic ciliate Ichthyophthirius multifiliis. The present study shows that the pro-inflammatory cytokines interleukin 1 beta (IL-1beta) and tumor necrosis factor alpha (TNF-alpha), the chemokine interleukin 8 (IL-8) and the type II IL-1 receptor (IL-1RII) are expressed in rainbow trout during an infection. In the skin an up to 17.8 times increase in the expression level of IL-1beta relative to uninfected controls was seen 4 days after infection. Upregulated expression of IL-1beta was likewise observed in the head kidney and spleen during the infection although to a lesser degree. TNF-alpha expression was seen in all examined tissues, with an increase in transcript level in the skin of up to 3.2 times the level in uninfected fish. A significant increase in the expression of IL-8 was evident in the skin and also to a lower extent in the head kidney and spleen. Expression of the down regulatory IL-1RII was seen in all three tissues. The potential involvement of the expression of these genes and their relevance in an infection with I. multifiliis are discussed.
authors.affiliation: Department of Veterinary Microbiology, Section of Fish Diseases, The Royal Veterinary and Agricultural University, Stigbøjlen 4.1, DK-1870 Frederiksberg C, Denmark. jsi@kvl.dk
authors.forename: J
authors.initials: J
authors.lastname: Sigh
authors.forename: T
authors.initials: T
authors.lastname: Lindenstrøm
authors.forename: K
authors.initials: K
authors.lastname: Buchmann
mesh_terms: D000818:Animals; D016770:Ciliophora Infections / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005393:Fish Diseases / Q000276:immunology* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000276:immunology*; D016803:Hymenostomatida / Q000276:immunology*; D007668:Kidney / Q000276:immunology / Q000378:metabolism; D017686:Oncorhynchus mykiss; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000276:immunology / Q000378:metabolism; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D013997:Time Factors
chemical_list: D016207:Cytokines; D017931:DNA Primers; D018076:DNA, Complementary