Role of VLDL receptor in the process of foam cell formation.
The role of very low density lipoprotein receptor (LVLDR) in the process of foam cell formation was investigated. After the primary cultured mouse peritoneal macrophages were incubated with VLDL, beta-VLDL or low density lipoprotein (LDL), respectively for 24 h and 48 h, foam cells formation was identified by oil red O staining and cellular contents of triglyceride (TG) and total cholesterol (TC) were determined. The mRNA levels of LDLR, LDLR related protein (ILRP) and VLDLR were detected by semi-quantitative RT-PCR. The results demonstrated that VLDL, beta-VLDL and LDL could increase the contents of TG and TC in macrophages. Cells treated with VLDL or beta-VLDL showed markedly increased expression of VLDLR and decreased expression of LDLR, whereas LRP was up-regulated slightly. For identifying the effect of VLDL receptor on cellular lipid accumulation, ldl-A7-VR cells, which expresses VLDLR and trace amount of LRP without functional LDLR, was used to incubate with lipoproteins for further examination. The results elucidated that the uptake of triglyceride-rich lipoprotein mediated by VLDLR plays an important role in accumulation of lipid and the formation of foam cells.
Qu
Shen
S
Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
Wu
Fan
F
Tian
Jun
J
Li
Yinghong
Y
Wang
Yan
Y
Wang
Yuzhe
Y
Zong
Yiqiang
Y
D000818:Animals; D001161:Arteriosclerosis / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D008078:Cholesterol, LDL / Q000378:metabolism / Q000494:pharmacology; D005260:Female; D005487:Foam Cells / Q000166:cytology* / Q000378:metabolism; D008079:Lipoproteins, VLDL / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D011973:Receptors, LDL / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism
D008078:Cholesterol, LDL; D008079:Lipoproteins, VLDL; D011973:Receptors, LDL; D014280:Triglycerides; C036053:VLDL receptor