title: Screening for topoisomerase I binding compounds by high-performance liquid chromatography-mass spectrometry.
abstract: A new rapid compound screening approach for topoisomerase I binding activity is presented. DNA topoisomerase I is used as a target protein to capture binding compounds from a mixture of combinatorial compounds by bioaffinity ultrafiltration. Using high-performance liquid chromatography combined with electrospray ionization mass spectrometry, small-molecule active compounds were identified. We also have successfully applied this method to identifying compounds from cells grown in culture.
authors.affiliation: Department of Food Science, Hangzhou University of Commerce, Hangzhou, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Qing
authors.initials: Q
authors.lastname: Gu
authors.forename: Xinle
authors.initials: X
authors.lastname: Liang
authors.forename: Yuanjiang
authors.initials: Y
authors.lastname: Pan
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004264:DNA Topoisomerases, Type I / Q000378:metabolism*; D008024:Ligands; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D008024:Ligands; D004264:DNA Topoisomerases, Type I