A new assay using surface plasmon resonance (SPR) to determine binding of the Lactobacillus acidophilus group to human colonic mucin.
A new binding assay to investigate the mechanism of adhesion of lactic acid bacteria to the human intestine was established by the surface plasmon resonance technique using a biosensor BIACORE1000. Cells of 26 strains of the Lactobacillus acidophilus group as analytes were eluted onto a sensor chip on which were immobilized biotinylated A-trisaccharide polymer probes having human A-type antigen [(GalNAcalpha1-3(Fucalpha1-2)Gal)-] or human colonic mucin of blood type A (HCM-A) as ligands. In the first screening, high adhesive affinity to the A-trisaccharide BP-probe was observed in L. acidophilus OLL2769, L. crispatus JCM8778, LA205 and LA206. In the second screening, which used HCM-A, only L. acidophilus OLL2769 and L. crispatus JCM8778 were selected as adhesive strains with specific binding ability to human A-antigen. The results indicated that some strains of the L. acidophilus group could recognize and bind the sugar chain of A-antigen structure on HCM.
Uchida
Hideaki
H
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Fujitani
Kenji
K
Kawai
Yasushi
Y
Kitazawa
Haruki
H
Horii
Akira
A
Shiiba
Kenichi
K
Saito
Kazuya
K
Saito
Tadao
T
D001422:Bacterial Adhesion / Q000502:physiology*; D015374:Biosensing Techniques / Q000379:methods*; D003106:Colon / Q000378:metabolism / Q000382:microbiology*; D006801:Humans; D007779:Lactobacillus acidophilus / Q000378:metabolism / Q000472:pathogenicity*; D009077:Mucins / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding; D020349:Surface Plasmon Resonance
D009077:Mucins