Rapid detection and identification of tomato vascular wilt pathogens using a DNA array.
Fusarium wilt, caused by Fusarium oxysporum f. sp. lycopersici, and Verticillium wilt, caused by either Verticillium albo-atrum or V. dahliae, are devastating diseases of tomato (Lycopersicon esculentum Mill.) found worldwide. Monitoring is the cornerstone of integrated pest management of any disease. The lack of rapid, accurate, and reliable means by which plant pathogens can be detected and identified is one of the main limitations in integrated disease management. In this paper, we describe the development of a molecular detection system, based on DNA array technology, for rapid and efficient detection of these vascular wilt pathogens. We demonstrate that by using this array these pathogens can be detected within 24 h from complex substrates like soil, plant material, and samples as they are collected by tomato growers in their greenhouses.
Lievens
B
B
Scientia Terrae Research Institute, Fortsesteenweg 30A, B-2860 Sint-Katelijne-Waver, Belgium. bli@scientiaterrae.org
Brouwer
M
M
Vanachter
A C R C
AC
Cammue
B P A
BP
Thomma
B P H J
BP
D004271:DNA, Fungal / Q000032:analysis*; D005670:Fusarium / Q000235:genetics* / Q000302:isolation & purification; D018551:Solanum lycopersicum / Q000382:microbiology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010935:Plant Diseases / Q000382:microbiology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020172:Verticillium / Q000235:genetics* / Q000302:isolation & purification
D004271:DNA, Fungal