Clastogenic effect of ethanol in chronic and abstinent alcoholics.
Alcoholism is one of the main causes of damage for human health, being relevant to study the induction of chromosomal aberrations (CA) by ethanol, and to investigate the individual susceptibility to diseases caused by alcoholism. A cytogenetic study was performed in human peripheral blood lymphocytes of 29 heavy chronic alcoholics, 11 alcoholics in abstinence, and 10 controls. The values of the chromosomal aberrations, mitotic indexes (MI) and proliferation indexes (PI) were determined. A molecular cytogenetic study was also carried out using fluorescence in situ hybridization (FISH) method with DNA library probes for chromosomes 1, 3 and 6, in lymphocytes from chronic alcoholic individuals in comparison with a control group. The results showed that the CA frequencies for chronic alcoholics (5.15 CA/100 cells) and alcoholics in abstinence (3.87 CA/100 cells) were higher than those obtained for control individuals (1.72 CA/100 cells). The mean translocation frequencies (equivalent to the genome) were calculated for six chronic alcoholics (0.267 translocations/100 cells) and six alcoholics in abstinence (0.167 translocations/100 cells), whose values were significantly higher than those observed for six control individuals (0.067 translocations/100 cells). The CA frequencies were not statistically different when smoker and non-smoker alcoholics were compared, indicating that although the smoking habit had significantly increased (four-fold) the CA frequency in healthy control individuals, a lack of interaction effect was observed within the group of alcoholics when smokers and non-smokers were compared. The CA frequencies presented by alcoholics in abstinence were similar to those obtained for chronic alcoholics. Therefore, chronic ethanol intoxication can lead to chromosome damage and disturbances in the metabolism of endogenous and exogenous compounds, which may persist for a long time, and constitute a relevant factor of risk for the development of neoplasias.
Burim
Regislaine Valéria
RV
Department of Genetics, Faculty of Medicine of Ribeirão Preto, São Paulo University, Ribeirão Preto, São Paulo, Brazil. cstaka@usp.br
Canalle
Renata
R
Takahashi
Catarina Satie
CS
Tavares
Denise Crispim
DC
Martinelli Ad
Ana de Lôurdes Candolo
Ade L
Sakamoto-Hojo
Elza Tiemi
ET
D000437:Alcoholism / Q000235:genetics*; D016022:Case-Control Studies; D002869:Chromosome Aberrations; D002908:Chronic Disease; D000431:Ethanol / Q000633:toxicity*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009153:Mutagens / Q000633:toxicity*; D014178:Translocation, Genetic
D009153:Mutagens; D000431:Ethanol