Protein kinase C beta is dispensable for TCR-signaling.
PKCbeta has been established to be essential in B cell receptor (BCR) signaling. Additionally, a critical role of PKCbeta in TCR/CD28-stimulated regulation of IL-2 gene transcription but also exocytotic IL-2 secretion was observed in leukemic T cell lines. To now study the physiological function of PKCbeta in primary CD3(+) T cells, we used our established PKCbeta null mice. Unexpectantly, we did not reveal any defect in the development and function of T cells. Proliferative responses as well as IL-2 cytokine secretion of PKCbeta-deficient CD3(+) T cells induced by allogenic MHC, plate-bound anti-CD3 antibodies (with or without anti-CD28 costimulation), or mitogenic stimuli such as phorbol ester and Ca(2+) ionophore were comparable with wild-type controls. Thus, PKCbeta-deficient T cells had similar physiological thresholds for activation in vitro. These findings suggest that PKCbeta plays a redundant role in TCR-induced regulation of IL-2 cytokine production and T cell proliferation.
Thuille
Nikolaus
N
Department for Medical Biology and Human Genetics, Innsbruck Medical University, Schoepfstrasse 41, A-6020 Innsbruck, Austria.
Gruber
Thomas
T
Böck
Günther
G
Leitges
Michael
M
Baier
Gottfried
G
D000818:Animals; D018106:CD28 Antigens / Q000276:immunology; D002455:Cell Division; D017930:Genes, Reporter; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics / Q000378:metabolism; D015759:Ionomycin / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D007527:Isoenzymes / Q000235:genetics / Q000502:physiology; D019169:Jurkat Cells; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D016853:Muromonab-CD3 / Q000494:pharmacology; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000235:genetics; D011493:Protein Kinase C / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D000074121:Protein Kinase C-theta; D011948:Receptors, Antigen, T-Cell / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000502:physiology; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology; D014162:Transfection
D018106:CD28 Antigens; D007376:Interleukin-2; D007476:Ionophores; D007527:Isoenzymes; D016853:Muromonab-CD3; D011948:Receptors, Antigen, T-Cell; D011993:Recombinant Fusion Proteins; D015240:Phorbol 12,13-Dibutyrate; D015759:Ionomycin; D008156:Luciferases; C076233:PRKCQ protein, human; C489593:Prkcq protein, mouse; D011493:Protein Kinase C; D000074121:Protein Kinase C-theta