title: [Study on the affinities of antagonistic peptides with HAb18G/CD147 on the hepatocellular carcinoma cells].
abstract: OBJECTIVE
  To study the affinities of antagonistic peptides with HAb18G/CD147 on the hepatocellular carcinoma cells.
  
  
  METHODS
  Fluorescence-activated cell sorting analysis(FACS) was used to detect the expression of HAb18G antigen on HHCC and SMMC7721 cells. Antagonistic peptides(APs) AP-1, AP-2 and AP-6 were labelled with biotin at their N-terminals. The binding abilities of APs to HHCC or SMMC7721 cells were determined by FACS and confocal microscope.
  
  
  RESULTS
  HAb18G antigen was highly expressed on the HHCC and SMMC7721 cells. AP-6 has the highest affinity for HHCC and SMMC7721 cells compared with AP-1 and AP-2.
  
  
  CONCLUSIONS
  Antagonistic peptides of HAb18G/CD147 have high affinities with HAb18G/CD147 on the hepatocellular carcinoma cells.
authors.affiliation: Cell Engineering Research Centre, Fourth Military Medical University, Xi'an 710032,China.
authors.forename: Ai-Rong
authors.initials: AR
authors.lastname: Qian
authors.forename: Peng
authors.initials: P
authors.lastname: Shang
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Fan
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Zhu
authors.forename: Zhi-Nan
authors.initials: ZN
authors.lastname: Chen
mesh_terms: D051926:Basigin / Q000378:metabolism; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D045744:Cell Line, Tumor; D006801:Humans; D008113:Liver Neoplasms; D010455:Peptides
chemical_list: D010455:Peptides; D051926:Basigin