A new ribonuclease from the black oyster mushroom Pleurotus ostreatus.
A ribonuclease that is co-specific for poly C and poly U has been isolated from the fruiting bodies of the black oyster mushroom. The enzyme possesses a molecular mass of 14 kDa, and is unadsorbed on DEAE-cellulose and adsorbed on Affi-gel blue gel and CM-cellulose. A pH of 7 is required for the enzyme to exhibit maximal activity. The activity of the enzyme does not vary appreciably over the temperature range 30-60 degrees C, but drops when the temperature is reduced to 20 degrees C or raised to and above 70 degrees C. The ribonuclease does not exert any inhibitory activity toward HIV-1 reverse transcriptase.
Wang
H X
HX
Department of Microbiology, China Agricultural University, Beijing, and State Key Laboratory of Agrobiotechnology, Beijing, China.
Ng
T B
TB
D002853:Chromatography, Liquid; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D008970:Molecular Weight; D020076:Pleurotus / Q000201:enzymology* / Q000254:growth & development; D011066:Poly C / Q000737:chemistry; D011072:Poly U / Q000737:chemistry; D012260:Ribonucleases / Q000737:chemistry* / Q000302:isolation & purification*; D013379:Substrate Specificity
D005656:Fungal Proteins; D011072:Poly U; D011066:Poly C; D012260:Ribonucleases