title: In vivo and in vitro analysis of the resistance against viral haemorrhagic septicaemia virus in Japanese flounder (Paralichthys olivaceus) precedingly infected with aquabirnavirus.
abstract: The resistance of Japanese flounder (Paralichthys olivaceus Temminck et Schlegel) against a viral haemorrhagic septicaemia virus (VHSV) challenge induced by a preceding non-lethal aquabirnavirus (ABV) challenge was investigated through experimental dual-infections with different intervals between the two challenges. The non-specific protection conferred by the primary ABV infection against the secondary VHSV infection commenced at Day 3 and persisted up to Day 14 but vanished at Day 21 post-ABV challenge. The in vitro assay using HINAE (hirame natural embryo) cells demonstrated anti-VHSV activity in the serum of ABV-challenged flounder from Day 1 to Day 14 but not at Day 21 post-ABV challenge. A high expression of a Mx gene, a molecular marker of type I interferon(s) (IFN) occurred in the head kidneys of ABV-challenged flounder from Day 1 to Day 7. These results suggest that the non-specific protection against the secondary VHSV infection in flounder was due to IFN(s) induced by the primary ABV infection.
authors.affiliation: Graduate School of Biosphere Science, Hiroshima University, Higashi-hiroshima 739-8528, Japan.
authors.forename: Rolando
authors.initials: R
authors.lastname: Pakingking
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Okinaka
authors.forename: Koh-Ichiro
authors.initials: K
authors.lastname: Mori
authors.forename: Misao
authors.initials: M
authors.lastname: Arimoto
authors.forename: Kiyokuni
authors.initials: K
authors.lastname: Muroga
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Nakai
mesh_terms: D000818:Animals; D019201:Aquabirnavirus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005432:Flounder; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D031941:Hemorrhagic Septicemia, Viral / Q000276:immunology*; D007113:Immunity, Innate; D007372:Interferons / Q000097:blood; D007668:Kidney / Q000378:metabolism / Q000821:virology; D007700:Kinetics; D064547:Myxovirus Resistance Proteins; D029141:Novirhabdovirus / Q000276:immunology*; D013997:Time Factors
chemical_list: D064547:Myxovirus Resistance Proteins; D007372:Interferons; D019204:GTP-Binding Proteins