Cell-free protein synthesis for proteomics.
The use of cell-free expression systems as an alternative to cell-based methods for protein production is greatly facilitating studies of protein functions. Recent improvements to cell-free systems, and the development of cell-free protein display and microarray technologies, have led to cell-free protein synthesis becoming a powerful tool for large-scale analysis of proteins. This paper reviews the most commonly used cell-free systems and their applications in proteomics.
Jackson
Alison M
AM
Discerna Ltd., Babraham Research Campus, Babraham, Cambridge CB2 4AT, UK.
Boutell
Joe
J
Cooley
Neil
N
He
Mingyue
M
D000818:Animals; D002474:Cell-Free System; D016678:Genome; D006801:Humans; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D040081:Protein Array Analysis / Q000379:methods*; D017510:Protein Folding; D020543:Proteome; D040901:Proteomics / Q000295:instrumentation* / Q000379:methods*; D012333:RNA, Messenger / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism
D020543:Proteome; D012333:RNA, Messenger