Expression pattern of the HMGB1 gene in sarcomas of the dog.
BACKGROUND
The human high mobility group protein B1 (HMGB1) has attracted considerable interest among oncologists because it sensitises cancer cells to the anticancer drug cisplatin by shielding cisplatin-DNA adducts from nucleotide excision repair.


METHODS
Since cisplatin is the cornerstone of adjuvant systemic therapy for osteosarcomas, in both humans and dogs, the expression pattern of the HMGB1 gene in seven canine sarcomas was investigated by Northern blot analysis and semi-quantitative RT-PCR.


RESULTS
A strong intertumoural variation of HMGB1 expression was detected by Northern blot analysis and confirmed by the semi-quantitative RT-PCR established herein.


CONCLUSIONS
The observed variations of HMGB1 expression in canine sarcomas emphasises the role of HMGB1 as a potential marker of clinical interest as its expression level may predict the clinical outcome of therapies based on cisplatin. The semi-quantitative RT-PCR established allows a quick and convenient determination of the HMGB1 expression level as necessary for clinical applications.
Meyer
Britta
B
alcedo biotech GmbH, Leobener Strasse ZHG, D-28359 Bremen, Germany.
Murua Escobar
Hugo
H
Hauke
Sven
S
Richter
Andreas
A
Winkler
Susanne
S
Rogalla
Piere
P
Flohr
Aljoscha M
AM
Bullerdiek
Jörn
J
Nolte
Ingo
I
D000818:Animals; D015152:Blotting, Northern; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism / Q000662:veterinary; D004283:Dog Diseases / Q000235:genetics* / Q000378:metabolism; D004285:Dogs; D005260:Female; D005354:Fibrosarcoma / Q000235:genetics / Q000378:metabolism / Q000662:veterinary; D015972:Gene Expression Regulation, Neoplastic; D024243:HMGB1 Protein / Q000096:biosynthesis / Q000235:genetics*; D007890:Leiomyosarcoma / Q000235:genetics / Q000378:metabolism / Q000662:veterinary; D008297:Male; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism / Q000662:veterinary; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012509:Sarcoma / Q000235:genetics* / Q000378:metabolism / Q000662:veterinary*
D024243:HMGB1 Protein; D012333:RNA, Messenger