Low-molecular-weight, biologically active compounds from marine Pseudoalteromonas species.
We have examined the ability of marine Proteobacteria from the Pseudoalteromonas genus and Alteromonas macleodii to produce low-molecular-weight, biologically active compounds with antimicrobial and surface-active properties. A new marine bacterium, Pseudoalteromonas issachenkonii, exhibited a high level of biological activity and produced antifungal and hemolytic compounds. A detailed spectroscopic investigation based on UV, IR, high-resolution mass spectrometry, and 2D 1H and 13C nuclear magnetic resonance revealed that the former was indole-2,3-dione (isatin). The chemical structure of red-brown pigment (C9H7N3OS3) responsible for hemolytic activity remained unclear. Four of the 15 strains studied (P. luteoviolacea, P. rubra, P. undina, and P. issachenkonii) produced cell-bound, two (P. elaykovii and P. carrageenovora) produced extracellular, and one strain (P. citrea) produced cell-bound and extracellular fatty acids and phospholipids with surface activity. Neither peptides nor glycolipids with surface activity were detected.
Kalinovskaya
Nataliya I
NI
Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Pr. 100 Let Vladivostoku 159, Russian Federation.
Ivanova
Elena P
EP
Alexeeva
Yulia V
YV
Gorshkova
Nataliya M
NM
Kuznetsova
Tatyana A
TA
Dmitrenok
Andrey S
AS
Nicolau
Dan V
DV
D020591:Alteromonas / Q000737:chemistry / Q000302:isolation & purification; D000935:Antifungal Agents / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D005227:Fatty Acids / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000633:toxicity; D006460:Hemolysin Proteins / Q000302:isolation & purification* / Q000378:metabolism / Q000633:toxicity; D007510:Isatin / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010743:Phospholipids / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000633:toxicity; D010860:Pigments, Biological / Q000302:isolation & purification* / Q000378:metabolism / Q000633:toxicity; D031161:Pseudoalteromonas / Q000737:chemistry* / Q000302:isolation & purification*; D013501:Surface-Active Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000633:toxicity; D014871:Water Microbiology
D000935:Antifungal Agents; D005227:Fatty Acids; D006460:Hemolysin Proteins; D010743:Phospholipids; D010860:Pigments, Biological; D013501:Surface-Active Agents; D007510:Isatin