Recognition of HLA-A3 and HLA-A11 by KIR3DL2 is peptide-specific.
The recognition of MHC class I molecules by killer cell immunoglobulin-like receptors (KIR) is central to the control of NK cell function and can also modulate the CTL activation threshold. Among KIR receptors, KIR3DL2 is thought to interact with HLA-A3 and -A11, although direct evidence has been lacking. In this study, we show that HLA-A3 and -A11 tetramers specifically bind to KIR3DL2*001 transfectants and that this recognition is peptide-specific. Single amino acid substitutions in the nonamer peptide underline a critical role for residue 8 in recognition of KIR3DL2. However, the role of this interaction in vivo still remains to be established.
Hansasuta
Pokrath
P
Faculty of Medicine, King Chulalongkorn Memorial Hospital, Chulalongkorn University, Bangkok, Thailand.
Dong
Tao
T
Thananchai
Hathairat
H
Weekes
Michael
M
Willberg
Christian
C
Aldemir
Hatice
H
Rowland-Jones
Sarah
S
Braud
Veronique M
VM
D005434:Flow Cytometry; D015234:HLA-A Antigens / Q000276:immunology*; D059870:HLA-A11 Antigen; D015791:HLA-A3 Antigen / Q000276:immunology*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D010446:Peptide Fragments; D011487:Protein Conformation; D011971:Receptors, Immunologic / Q000276:immunology*; D054340:Receptors, KIR; D054347:Receptors, KIR3DL2; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014162:Transfection
D015234:HLA-A Antigens; D059870:HLA-A11 Antigen; D015791:HLA-A3 Antigen; C515084:KIR3DL2 protein, human; D010446:Peptide Fragments; D011971:Receptors, Immunologic; D054340:Receptors, KIR; D054347:Receptors, KIR3DL2