title: Ribosomal crystallography: a flexible nucleotide anchoring tRNA translocation, facilitates peptide-bond formation, chirality discrimination and antibiotics synergism.
abstract: The linkage between internal ribosomal symmetry and transfer RNA (tRNA) positioning confirmed positional catalysis of amino-acid polymerization. Peptide bonds are formed concurrently with tRNA-3' end rotatory motion, in conjunction with the overall messenger RNA (mRNA)/tRNA translocation. Accurate substrate alignment, mandatory for the processivity of protein biosynthesis, is governed by remote interactions. Inherent flexibility of a conserved nucleotide, anchoring the rotatory motion, facilitates chirality discrimination and antibiotics synergism. Potential tRNA interactions explain the universality of the tRNA CCA-end and P-site preference of initial tRNA. The interactions of protein L2 tail with the symmetry-related region periphery explain its conservation and its contributions to nascent chain elongation.
authors.affiliation: Department of Structural Biology, The Weizmann Institute, 76100 Rehovot, Israel.
authors.forename: Ilana
authors.initials: I
authors.lastname: Agmon
authors.forename: Maya
authors.initials: M
authors.lastname: Amit
authors.forename: Tamar
authors.initials: T
authors.lastname: Auerbach
authors.forename: Anat
authors.initials: A
authors.lastname: Bashan
authors.forename: David
authors.initials: D
authors.lastname: Baram
authors.forename: Heike
authors.initials: H
authors.lastname: Bartels
authors.forename: Rita
authors.initials: R
authors.lastname: Berisio
authors.forename: Inbal
authors.initials: I
authors.lastname: Greenberg
authors.forename: Joerg
authors.initials: J
authors.lastname: Harms
authors.forename: Harly A S
authors.initials: HA
authors.lastname: Hansen
authors.forename: Maggie
authors.initials: M
authors.lastname: Kessler
authors.forename: Erez
authors.initials: E
authors.lastname: Pyetan
authors.forename: Frank
authors.initials: F
authors.lastname: Schluenzen
authors.forename: Assa
authors.initials: A
authors.lastname: Sittner
authors.forename: Ada
authors.initials: A
authors.lastname: Yonath
authors.forename: Raz
authors.initials: R
authors.lastname: Zarivach
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry; D017963:Azithromycin / Q000494:pharmacology; D002384:Catalysis; D018360:Crystallography, X-Ray / Q000379:methods*; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry; D020033:Protein Isoforms; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D012333:RNA, Messenger / Q000378:metabolism; D012343:RNA, Transfer / Q000737:chemistry / Q000378:metabolism; D012270:Ribosomes / Q000737:chemistry* / Q000648:ultrastructure*; D013379:Substrate Specificity; D014769:Virginiamycin / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D000900:Anti-Bacterial Agents; D010455:Peptides; D020033:Protein Isoforms; D012333:RNA, Messenger; D014769:Virginiamycin; C062940:quinupristin-dalfopristin; D017963:Azithromycin; D012343:RNA, Transfer