title: Production of polyclonal antibodies against territrem B and detection of territrem B in the conidia of Aspergillus terreus 23-1 by immunoelectron microscopy.
abstract: Territrem B, a fungal metabolite isolated from Aspergillums terreus 23-1, is a tremorgenic mycotoxin. Immunoelectron microscopy using anti-territrem B polyclonal antibody was used to detect territrem B in the fungal body of A. terreus 23-1 at different times of culture without shaking on potato dextrose (PD) agar medium. The anti-territrem B serum was produced by immunization of rabbits with 4beta-hydroxymethyl-4beta-demethylterritrem B-sccinate bound by a linker to keyhole limpet hemocyanin. This antiserum recognized territrems and immunoelectron microscopy using this antiserum, and colloidal gold-conjugated anti-rabbit IgG antibodies showed that territrem B was localized to the fungal body of A. terreus 23-1. Territrem B was first seen in the cytoplasm of the conidia after 4 days' culture on PD agar medium. Maximal territrem B production in the conidia was seen on the 14th day of culture; however, territrem B was not formed in the hyphae at any stage of culture. These results are consistent with the previous finding that the formation of territrems is related to fungal sporulation.
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei, Taiwan. fcpeng@ha.mc.ntu.edu.tw
authors.forename: Fu-Chuo
authors.initials: FC
authors.lastname: Peng
authors.forename: Chiung-Hsiang
authors.initials: CH
authors.lastname: Cheng
authors.forename: Jen-Cheng
authors.initials: JC
authors.lastname: Liu
authors.forename: Ming-Fang
authors.initials: MF
authors.lastname: Wu
authors.forename: Su-Huei
authors.initials: SH
authors.lastname: Lee
authors.forename: Huei-Ju
authors.initials: HJ
authors.lastname: Pan
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D001230:Aspergillus / Q000737:chemistry*; D006241:Haptens / Q000276:immunology; D007106:Immune Sera / Q000096:biosynthesis; D016253:Microscopy, Immunoelectron; D011714:Pyrans / Q000032:analysis* / Q000276:immunology*; D011817:Rabbits; D013172:Spores, Fungal / Q000737:chemistry
chemical_list: D000906:Antibodies; D006241:Haptens; D007106:Immune Sera; D011714:Pyrans; C040405:territrem B