[Study on detecting the sensitivity of tumor cells to chemotherapeutic agents by immunohistochemical staining with anti-ssDNA mAb].
OBJECTIVE
To explore the possibility of application of self-made anti-ssDNA mAb to detecting the apoptosis of tumor cells induced by chemotherapeutic drugs and evaluate the sensitivity of tumor cells to chemotherapeutic drugs from a apoptotic rate.


METHODS
Apoptosis of both K562 and Hep-2 cells were induced by maximum plasma concentrations of 9 different chemotherapeutic drugs. The apoptosis of tumor cells was detected by immunohistochemical ( IHC) staining with anti-ssDNA mAb and their apoptotic rate were calculated.


RESULTS
The apoptotic cells could be differentiated from non-apoptotic cells by the HIC staining with anti-ssDNAmAb. The apoptosis of tumor cells were induced by chemotherapeutic drugs, but apoptotic extent of different tumor cells, even the same one tumor cell, was distinct when they exposed to all kinds of anti-tumor drugs.


CONCLUSIONS
The combination of anti-ssDNA mAb with IHC staining can be used to detect the sensitivity of tumor cells to chemotherapeutic drugs.
Wu
Xiao-yan
XY
Institute of Immunology, Weifang Medical College, Weifang 261042, China.
Xu
Zhi-wei
ZW
Wu
Guo-qing
GQ
Miao
Nai-fa
NF
Feng
Yong-tang
YT
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D004277:DNA, Single-Stranded / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D020014:K562 Cells / Q000187:drug effects; D051379:Mice; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D004277:DNA, Single-Stranded