[The effects of Zheng's plant-protein on the proliferation of lymphocytes from patients with breast cancer and precancer].
OBJECTIVE
To study the effect of Zheng's plant-protein(ZPP) and phytohemagglutinin(PHA) on proliferation of peripheral blood lymphocytes (PBL) and it's application value on the early diagnosis and therapy of breast cancer.


METHODS
The proliferation assay was used (3)H-TdR incorporation test. The phenotypes of PBL were analysised by flow cytometry(FCM).


RESULTS
The ZPP significantly and selectively enhanced proliferation activity of PBL of primary breast and precancer cases. Their stimulation indexes(SI) were 3.00+/-1.45 and 2.53+/-0.80, while the SI of simple hyperplasia cases and normal subjecties were 1.18+/-0.47 and 1.06+/-0.91. The former were significantly higher than the latter (P<0.01). The ZPP was also enhanced the expression level of CD3(+),CD4(+) and CD8(+) of PBL.


CONCLUSIONS
This results suggest that ZPP might be a beast cancer common antigen. These activity might be used in clinical implication to diagnosis and treatment of breast cancer.
Ren
Jin-Rong
JR
Department of Immunology, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011,China.
Shan
Bao-En
BE
Zheng
Zhen-Hai
ZH
Liu
Lu-Ning
LN
He
Lan-Xin
LX
Ai
Jun
J
D001943:Breast Neoplasms / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism; D010641:Phenotype