title: Composite vascularized skin/bone graft model: a viable source for vascularized bone marrow transplantation.
abstract: In this study, we introduce a new model for vascularized skin and bone marrow transplantation. Twenty-five Lewis (RT1(1)) rats were studied. Anatomic dissection studies were performed in 5 animals. In the experimental group, 10 isograft transplantations were performed between Lewis rats. Combined groin skin and femoral bone flaps were transplanted based on the femoral artery and vein. Transplants were evaluated on a daily basis. All flaps survived without problems over 100 days posttransplant. The skin component remained pink and pliable, and grew new hair. Histological examination of the femoral bone (except the femoral head) revealed active hematopoiesis with a viable compact and cancellous bone components on day 100 posttransplant. This model can be applied to tolerance induction studies across the major Histocompatibility (MHC) barrier, where bone will serve as donor of stem and progenitor cells, and the skin flap will serve as a monitor of graft rejection.
authors.affiliation: Department of Plastic Surgery, Cleveland Clinic Foundation, Cleveland, OH 44195, USA. siemiom@ccf.org
authors.forename: Maria
authors.initials: M
authors.lastname: Siemionow
authors.forename: Betul G
authors.initials: BG
authors.lastname: Ulusal
authors.forename: Selahattin
authors.initials: S
authors.lastname: Ozmen
authors.forename: Ali E
authors.initials: AE
authors.lastname: Ulusal
authors.forename: Kagan
authors.initials: K
authors.lastname: Ozer
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D016025:Bone Transplantation / Q000379:methods / Q000473:pathology; D002678:Chimera; D004195:Disease Models, Animal; D005260:Female; D005269:Femur / Q000098:blood supply* / Q000637:transplantation*; D006084:Graft Rejection; D006085:Graft Survival; D008297:Male; D008833:Microcirculation; D051381:Rats; D011917:Rats, Inbred Lew; D012680:Sensitivity and Specificity; D016038:Skin Transplantation / Q000379:methods / Q000473:pathology; D020858:Tissue and Organ Harvesting; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic