Aspects of the barley seed proteome during development and germination.
Analysis of the water-soluble barley seed proteome has led to the identification of proteins by MS in the major spots on two-dimensional gels covering the pI ranges 4-7 and 6-11. This provides the basis for in-depth studies of proteome changes during seed development and germination, tissue-specific proteomes, cultivar differences related to quality parameters, analysis of the genetic basis for spot variations and targeted investigations of specific proteins.
Finnie
C
C
Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500 Valby, Denmark. csf@biocentrum.dtu.dk
Maeda
K
K
ØStergaard
O
O
Bak-Jensen
K S
KS
Larsen
J
J
Svensson
B
B
D015180:Electrophoresis, Gel, Two-Dimensional; D018525:Germination; D001467:Hordeum / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry; D010940:Plant Proteins / Q000737:chemistry*; D020543:Proteome; D012639:Seeds / Q000378:metabolism*; D013045:Species Specificity; D014867:Water / Q000737:chemistry
D010940:Plant Proteins; D020543:Proteome; D014867:Water