title: A highly discriminating quencher-free molecular beacon for probing DNA.
abstract: We inserted a fluorene-labeled deoxyuridine derivative, synthesized using Sonogashira coupling, efficiently into the loop region of a DNA hairpin using phosphoramidite chemistry. This molecular beacon, which features no additional fluorescence quencher, discriminates between perfect and one-base-mismatched base pairing by changes in its fluorescence intensity. The discrimination factor is 14.7 for the recognition of a single (A/C) base mismatch.
authors.affiliation: Department of Chemistry, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea.
authors.forename: Gil Tae
authors.initials: GT
authors.lastname: Hwang
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Seo
authors.forename: Byeang Hyean
authors.initials: BH
authors.lastname: Kim
mesh_terms: D020137:Base Pair Mismatch; D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D005449:Fluorenes / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009705:Nucleosides / Q000138:chemical synthesis; D009841:Oligonucleotides / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D005449:Fluorenes; D009705:Nucleosides; D009841:Oligonucleotides; D004247:DNA