New developments in automated cytogenetic imaging: unattended scoring of dicentric chromosomes, micronuclei, single cell gel electrophoresis, and fluorescence signals.
The quantification of DNA damage, both in vivo and in vitro, can be very time consuming, since large amounts of samples need to be scored. Additional uncertainties may arise due to the lack of documentation or by scoring biases. Image analysis automation is a possible strategy to cope with these difficulties and to generate a new quality of reproducibility. In this communication we collected some recent results obtained with the automated scanning platform Metafer, covering applications that are being used in radiation research, biological dosimetry, DNA repair research and environmental mutagenesis studies. We can show that the automated scoring for dicentric chromosomes, for micronuclei, and for Comet assay cells produce reliable and reproducible results, which prove the usability of automated scanning in the above mentioned research fields.
Schunck
C
C
MetaSystems GmbH, Altlussheim, Ulm, Germany. cschunck@metasystems.de
Johannes
T
T
Varga
D
D
Lörch
T
T
Plesch
A
A
D000818:Animals; D001331:Automation; D002452:Cell Count / Q000295:instrumentation; D002467:Cell Nucleus / Q000648:ultrastructure; D002869:Chromosome Aberrations; D020552:Comet Assay / Q000295:instrumentation; D020732:Cytogenetic Analysis / Q000295:instrumentation*; D004249:DNA Damage; D004867:Equipment Design; D005720:Gamma Rays; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation*; D008214:Lymphocytes / Q000528:radiation effects / Q000648:ultrastructure; D008838:Microcomputers; D015162:Micronucleus Tests / Q000295:instrumentation; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D009152:Mutagenicity Tests / Q000295:instrumentation; D011874:Radiometry / Q000295:instrumentation / Q000379:methods; D015203:Reproducibility of Results