title: Interactive effects of microbial transglutaminase and recombinant cystatin on the mackerel and hairtail muscle protein.
abstract: Interactive effects of microbial transglutaminase (MTGase) and recombinant cystatin on the mackerel and hairtail water soluble protein (WSP), salt soluble protein (SSP), and muscle protein (MP) were investigated. According to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and enzymic activity analyses, cross-linking of mackerel and hairtail myosin heavy chain and low molecular mass compounds and formation of epsilon-(gamma-glutamyl)lysine cross-links were observed on samples with MTGase, while the recombinant cystatin could effectively inhibit the cathepsins and subsequently prevent degradation of proteins during setting. The cathepsins and MTGase activities in WSP, SSP, and MP solutions decreased, but the recombinant cystatin activity increased during setting at 45 degrees C.
authors.affiliation: Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan 20224, Republic of China. sjiang@mail.ntou.edu.tw
authors.forename: Shann-Tzong
authors.initials: ST
authors.lastname: Jiang
authors.forename: Jung-Feng
authors.initials: JF
authors.lastname: Hsieh
authors.forename: Guo-Jane
authors.initials: GJ
authors.lastname: Tsai
mesh_terms: D000818:Animals; D002403:Cathepsins / Q000037:antagonists & inhibitors; D015891:Cystatins / Q000494:pharmacology*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005396:Fish Products; D009124:Muscle Proteins / Q000378:metabolism*; D010473:Perciformes; D011994:Recombinant Proteins; D012995:Solubility; D011503:Transglutaminases / Q000378:metabolism*
chemical_list: D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors; D009124:Muscle Proteins; D011994:Recombinant Proteins; D011503:Transglutaminases; D002403:Cathepsins