Nucleotide sequence analysis of the recA gene and discrimination of the three isolates of urease-positive thermophilic Campylobacter (UPTC) isolated from seagulls (Larus spp.) in Northern Ireland.
Nucleotide sequencing after TA cloning of the amplicon of the almost-full length recA gene from three strains of UPTC (A1, A2, and A3) isolated from seagulls in Northern Ireland, the phenotypical and genotypical characteristics of which have been demonstrated to be indistinguishable, clarified nucleotide differences at three nucleotide positions among the three strains. In conclusion, the nucleotide sequences of the recA gene were found to discriminate among the three strains of UPTC, A1, A2, and A3, which are indistinguishable phenotypically and genotypically. Thus, the present study strongly suggests that nucleotide sequence data of the amplicon of a suitable gene or region could aid in discriminating among isolates of the UPTC group, which are indistinguishable phenotypically and genotypically.
Matsuda
M
M
Laboratory of Molecular Biology, School of Environmental Health Sciences, Azabu University, Fuchinobe 1-17-71 [corrected] Sagamihara 229-8501, Japan. matsuda@azabu-u.ac.jp
Tai
K
K
Moore
J E
JE
Millar
B C
BC
Murayama
O
O
D000818:Animals; D002167:Campylobacter / Q000145:classification* / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D046091:Charadriiformes / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D005798:Genes, Bacterial; D005838:Genotype; D008969:Molecular Sequence Data; D009660:Northern Ireland; D010641:Phenotype; D011938:Rec A Recombinases / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014510:Urease / Q000096:biosynthesis*
D004269:DNA, Bacterial; D011938:Rec A Recombinases; D014510:Urease