Establishment and characterization of a human malignant mesothelioma cell line (HMMME).
A cell line designated "HMMME" was established from the pleural fluids of a malignant mesothelioma patient. This line grew well without interruption for 12 years and was subcultured over 200 times. The cells were spindle and roundish in shape and displayed a monolayer sheet in an epithelial pavement cell arrangement. They were neoplastic, had pleomorphic features, and easily formed multilayering without contact inhibition. The cell cytoplasm was strongly positive against anti-vimentin, anti-calretinin and anti-pan-keratin, but negative against anti-BerEP4. The cells proliferated rapidly, and the population doubling time was about 42 hours. Their chromosome number showed a wide distribution of aneuploidy with a mode in the diploid range; many marker chromosomes were observed. The cultured cells were easily transplanted into the subcutaneous of nude mice and produced a tumor classified as a malignant mesothelioma.
Ishiwata
Isamu
I
Ishiwata Obstetrics and Gynecology Hospital, Ibaraki-ken, Japan.
Ishiwata
Chieko
C
Anzo
Mitsuru
M
Minami
Rie
R
Kiguchi
Kazushige
K
Tachibana
Toshiaki
T
Ishikawa
Hiroshi
H
D000818:Animals; D064032:Calbindin 2; D002455:Cell Division; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000378:metabolism; D008654:Mesothelioma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
C576764:CALB2 protein, human; C576511:Calb2 protein, mouse; D064032:Calbindin 2; D064030:S100 Calcium Binding Protein G; D014746:Vimentin; D007633:Keratins