Trp-Lys-Tyr-Met-Val-Met stimulates phagocytosis via phospho-lipase D-dependent signaling in mouse dendritic cells.
Dendritic cells (DCs) play a key role in activating the immune response against invading pathogens as well as dying cells or tumors. Although the immune response can be initiated by the phagocytic activity by DCs, the molecular mechanism involved in this process has not been fully investigated. Trp-Lys-Tyr-Met-Val-Met-NH(2) (WKYMVM) stimulates the activation of phospholipase D (PLD) via Ca(2+) increase and protein kinase C activation in mouse DC cell line, DC2.4. WKYMVM stimulates the phagocytic activity, which is inhibited in the presence of N-butanol but not t-butanol in DC2.4 cells. Furthermore, the addition of phosphatidic acid, an enzymatic product of PLD activity, enhanced the phagocytic activity in DC2.4 cells. Since at least two of formyl peptide receptor (FPR) family (FPR1 and FPR2) are expressed in DC2.4 as well as in mouse bone marrow-derived dendritic cells, this study suggests that the activation of FPR family by WKYMVM stimulates the PLD activity resulting in phagocytic activity in DC2.4 cells.
Lee
Ha-Young
HY
Medical Research Center for Cancer Molecular Therapy, College of Medicine, Dong-A University, Busan 602-714, Korea.
Kang
Hyun Kyu
HK
Jo
Eun Jin
EJ
Kim
Jung Im
JI
Lee
Youl-Nam
YN
Lee
Sang Hwa
SH
Park
Yeong Min
YM
Ryu
Sung Ho
SH
Kwak
Jong-Young
JY
Bae
Yoe-Sik
YS
D020001:1-Butanol / Q000494:pharmacology; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects*; D016923:Cell Death / Q000276:immunology; D002460:Cell Line; D003141:Communicable Diseases / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D009369:Neoplasms / Q000276:immunology; D009842:Oligopeptides / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects*; D010712:Phosphatidic Acids / Q000494:pharmacology; D010739:Phospholipase D / Q000378:metabolism*; D044042:Receptors, Formyl Peptide / Q000378:metabolism*; D020002:tert-Butyl Alcohol / Q000494:pharmacology
D009842:Oligopeptides; D010712:Phosphatidic Acids; D044042:Receptors, Formyl Peptide; C113617:Trp-Lys-Tyr-Met-Val-Met; D020001:1-Butanol; D010739:Phospholipase D; D020002:tert-Butyl Alcohol