title: Isolation of granulocytes and mononuclear cells from the blood of dogs, cats, horses and cattle.
abstract: A simple discontinuous Percoll density-gradient technique was adapted for isolation of granulocytes and mononuclear cells from cats, dogs, horses and cattle. Separation was accomplished at low speeds using a standard tabletop centrifuge. Cell purity was 100% for both granulocytes and mononuclear cells and cell viability exceeded 95%. Percent recovery of leukocytes ranged from 69 to 83%.
authors.affiliation: Department of Veterinary Pathobiology College of Veterinary Medicine University of Minnesota St. Paul, Minnesota 55108.
authors.forename: D J
authors.initials: DJ
authors.lastname: Weiss
authors.forename: R
authors.initials: R
authors.lastname: Kraemer
authors.forename: K
authors.initials: K
authors.lastname: Schmit