Solasodine glycoside production by hairy root cultures of Physalis minima Linn.
Hairy root cultures of Physalis minima L. were developed using Agrobacterium rhizogenes, strain ATCC 15834 mediated transformation and grown in half strength of Murashige and Skoog medium containing 8% (w/v) sucrose. Media supplementation with 1 mg naphthalenacetic acid l(-1) and 1 mg benzyladenine increased solasodine glycoside up to 900 g dry wt, which was 20 times higher than that in the native root.
Putalun
Waraporn
W
Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand. waraporn@kku.ac.th
Prasamsiwamai
Preeyaporn
P
Tanaka
Hiroyuki
H
Shoyama
Yukihiro
Y
D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D032321:Physalis / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology*; D012231:Rhizobium / Q000502:physiology*; D012991:Solanaceous Alkaloids / Q000096:biosynthesis* / Q000302:isolation & purification; D013395:Sucrose / Q000378:metabolism
D010937:Plant Growth Regulators; D012991:Solanaceous Alkaloids; D013395:Sucrose; C026608:alpha-solamargine