Cationic oligopeptide-mediated delivery of dsRNA for post-transcriptional gene silencing in plant cells.
We have used cationic oligopeptide polyarginine-12mer (POA) to deliver double-stranded RNA (dsRNA), prepared in vitro, to tobacco (Nicotiana tabacum) suspension cells. POA interacts electrostatically with dsRNA to form a complex. When dsRNA for the GUS or NPTII gene was delivered into cells carrying the same genes, the corresponding mRNA was degraded. Using RNase protection assay we were able to detect 21-bp small interfering RNA in dsRNA/POA-treated cells. These results demonstrate that POA can be used to deliver dsRNA to induce post-transcriptional gene silencing in plant cells.
Unnamalai
Narayanan
N
Department of Biological Science, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Kang
Bong Gu
BG
Lee
Woo Sung
WS
D015152:Blotting, Northern; D002412:Cations; D002478:Cells, Cultured; D017343:Genes, Plant / Q000235:genetics; D009842:Oligopeptides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D018520:Plant Shoots / Q000166:cytology; D030821:Plants, Genetically Modified / Q000166:cytology; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000096:biosynthesis; D014026:Nicotiana / Q000166:cytology / Q000235:genetics* / Q000648:ultrastructure
D002412:Cations; D009842:Oligopeptides; D010455:Peptides; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C015462:polyarginine