Influence of antiplatelet substances on platelet-rich plasma.
OBJECTIVE
Platelets containing a number of growth factors (platelet-derived growth factor [PDGF], transforming growth factor-beta [TGF-beta], etc) can be obtained in high concentrations through centrifugal separation and are used in clinical applications as platelet-rich plasma (PRP). However, only a few studies have been conducted on the growth factors present in PRP. In this study, we focused on the concentrations of growth factors in PRP and clarified the influence of using antiplatelet substances in the process of platelet concentration to improve the concentration rate of growth factors in PRP.


METHODS
We made platelet pellets from whole blood obtained from humans with or without some antiplatelet substances (prostaglandin E1, aspirin, apyrase). Platelet pellets were resuspended in phosphate-buffered saline as platelet resuspensions. We measured PDGF and TGF-beta1 concentrations in the samples. In measurements, we had the samples treated to release growth factors from platelets to measure accurate concentrations.


RESULTS
PDGF and TGF-beta1 were concentrated to a mean of over 400% in the samples with antiplatelet substances as compared with the samples without antiplatelet substances.


CONCLUSIONS
The antiplatelet substances were effective for efficiently concentrating growth factors in platelets.
Yazawa
Masaki
M
Department of Plastic and Reconstructive Surgery, Keio University School of Medicine, Tokyo, Japan. yazawa@sc.itc.keio.ac.jp
Ogata
Hisao
H
Nakajima
Tatsuo
T
Watanabe
Naohide
N
D000328:Adult; D001792:Blood Platelets / Q000737:chemistry / Q000187:drug effects*; D002452:Cell Count; D006801:Humans; D066298:In Vitro Techniques; D010949:Plasma / Q000737:chemistry / Q000166:cytology*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D010982:Platelet-Derived Growth Factor / Q000032:analysis / Q000187:drug effects*; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000187:drug effects*
D010975:Platelet Aggregation Inhibitors; D010982:Platelet-Derived Growth Factor; D016212:Transforming Growth Factor beta