Simultaneous determination of aflatoxins, ochratoxin A, and zearalenone in grains by new immunoaffinity column/liquid chromatography.
The simultaneous determination of mycotoxins was performed in 3 steps: extraction, cleanup, and detection. For extraction, a mixture of acetonitrile-water (60 + 40, v/v) was proved appropriate. For cleanup, a new Afla-Ochra-Zea immunoaffinity column was used. After derivatization with trifluoroacetic acid, the mycotoxins aflatoxins, ochratoxin A (OTA), and zearalenone (ZEA) were determined simultaneously by liquid chromatography with fluorescence detection. The detection limits in different matrixes after cleanup with the new immunoaffinity column were very low: aflatoxins, 0.002-0.7 microg/kg; OTA, 0.07-0.25 microg/kg; ZEA, 1-3 microg/kg. The limits of determination were: aflatoxins, 0.25 microg/kg; OTA, 0.5 microg/kg; ZEA, 5 microg/kg. The recovery rates for aflatoxins, OTA, and ZEA for rye and rice were between 86 and 93% when a 0.5 g sample matter per immunoaffinity column was used.
Göbel
Roswitha
R
Institut für Veterinar-Pharmakologie und Toxikologie GmbH, Weissenseer Strasse 36 D-16321 Bernau, Germany. ivpt-bernau@t-online.de
Lusky
Klaus
K
D000348:Aflatoxins / Q000032:analysis*; D002846:Chromatography, Affinity; D002853:Chromatography, Liquid; D009793:Ochratoxins / Q000032:analysis*; D015025:Zearalenone / Q000032:analysis*
D000348:Aflatoxins; D009793:Ochratoxins; C025589:ochratoxin A; D015025:Zearalenone