The use of isotope-coded affinity tags (ICAT) to study organelle proteomes in Arabidopsis thaliana.
Organelle proteomics is the analysis of the protein contents of a subcellular compartment. Proteins identified in subcellular proteomic studies can only be assigned to an organelle if there are no contaminants present in the sample preparation. As a result, the majority of plant organelle proteomic studies have focused on the chloroplast and mitochondria, which can be isolated relatively easily. However, the isolation of components of the endomembrane system is far more difficult due to their similar sizes and densities. For this reason, quantitative proteomics methods are being developed to enable the assignment of proteins to a specific component of the endomembrane system without the need to obtain pure organelles.
Dunkley
T P J
TP
Department of Biochemistry, Cambridge Centre for Proteomics, University of Cambridge, Building O, Downing Site, Cambridge CB2 1QW, UK.
Dupree
P
P
Watson
R B
RB
Lilley
K S
KS
D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000032:analysis; D002462:Cell Membrane / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D007554:Isotopes; D008928:Mitochondria / Q000473:pathology; D015388:Organelles; D010455:Peptides / Q000737:chemistry; D010944:Plants / Q000378:metabolism; D020543:Proteome; D040901:Proteomics / Q000379:methods
D029681:Arabidopsis Proteins; D007554:Isotopes; D010455:Peptides; D020543:Proteome