title: Marek's disease vaccination, with turkey herpesvirus, and enrofloxacin modulate the activities of hepatic microsomal enzymes in broiler chickens.
abstract: Chickens were vaccinated against Marek's disease intramuscularly at one day of age. Enrofloxacin was given ad libitum in the drinking water at concentrations of 50, 100 and 250 mg/L from 8 days to 13 days of age when the animals were killed and the activities of cytochrome P-450 enzymes in the liver were measured. Vaccinated non-treated chickens served as a positive control. A negative control group was neither vaccinated nor treated. Vaccination decreased the activity of aniline hydroxylase and ethylmorphine N-demethylase in the positive control group. Subsequent application of enrofloxacin in the lowest concentration (50 mg/L) decreased, while that given at the highest level (250 mg/L) significantly increased the activity of the same microsomal enzymes. Relative liver weights and concentrations of proteins in 9000 x g supernatant were not affected by vaccination or treatment.
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia.
authors.forename: D
authors.initials: D
authors.lastname: Sakar
authors.forename: Estela
authors.initials: E
authors.lastname: Prukner-Radovcić
authors.forename: Andreja
authors.initials: A
authors.lastname: Prevendar Crnić
authors.forename: Jelena
authors.initials: J
authors.lastname: Pompe-Gotal
authors.forename: W L
authors.initials: WL
authors.lastname: Ragland
authors.forename: H
authors.initials: H
authors.lastname: Mazija
mesh_terms: D000815:Aniline Hydroxylase / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002645:Chickens; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000008:administration & dosage / Q000494:pharmacology*; D006564:Herpesviridae / Q000276:immunology*; D007273:Injections, Intramuscular / Q000662:veterinary; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008380:Marek Disease / Q000378:metabolism / Q000517:prevention & control*; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D015363:Quinolones / Q000008:administration & dosage / Q000494:pharmacology*; D014765:Viral Vaccines
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D015363:Quinolones; D014765:Viral Vaccines; D000077422:Enrofloxacin; D000815:Aniline Hydroxylase