Woronin body function in Magnaporthe grisea is essential for efficient pathogenesis and for survival during nitrogen starvation stress.
The Woronin body is a peroxisome-derived dense-core vesicle that is specific to several genera of filamentous ascomycetes, where it has been shown to seal septal pores in response to cellular damage. The Hexagonal peroxisome (Hex1) protein was recently identified as a major constituent of the Woronin body and shown to be responsible for self-assembly of the dense core of this organelle. Using a mutation in the Magnaporthe grisea HEX1 ortholog, we define a dual and essential function for Woronin bodies during the pathogenic phase of the rice blast fungus. We show that the Woronin body is initially required for proper development and function of appressoria (infection structures) and subsequently necessary for survival of infectious fungal hyphae during invasive growth and host colonization. Fungal mycelia lacking HEX1 function were unable to survive nitrogen starvation in vitro, suggesting that in planta growth defects are a consequence of the mutant's inability to cope with nutritional stress. Thus, Woronin body function provides the blast fungus with an important defense against the antagonistic and nutrient-limiting environment encountered within the host plant.
Soundararajan
Shanthi
S
Fungal Genomics Laboratory, Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604.
Jedd
Gregory
G
Li
Xiaolei
X
Ramos-Pamploña
Marilou
M
Chua
Nam H
NH
Naqvi
Naweed I
NI
D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005090:Exodeoxyribonucleases / Q000172:deficiency / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D001467:Hordeum / Q000382:microbiology; D025301:Hyphae / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D020082:Magnaporthe / Q000187:drug effects / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009584:Nitrogen / Q000494:pharmacology*; D019697:Onions / Q000382:microbiology; D012275:Oryza / Q000382:microbiology; D020675:Peroxisomes / Q000201:enzymology / Q000502:physiology*; D010935:Plant Diseases / Q000382:microbiology*; D010944:Plants / Q000382:microbiology*; D014774:Virulence
D004269:DNA, Bacterial; D005656:Fungal Proteins; C036483:T-DNA; D005090:Exodeoxyribonucleases; D009584:Nitrogen