6-Dimethylaminopurine (6-DMAP), which is used to produce most cloned animals, is mutagenic in Salmonella typhimurium TA1535.
6-Dimethylaminopurine (6-DMAP) and cycloheximide have recently been used for successful production of cloned mammals. We investigated whether 6-DMAP or cycloheximide are mutagenic agents in the Ames test. Whereas cycloheximide showed no differences compared to the negative control in any of the tester strains, 6-DMAP was clearly mutagenic in Salmonella typhimurium strain TA1535. Here, we strongly propose that innocuous chemicals be used in the production of cloned animals.
Katoh
Motoe
M
Genotoxic Risk Assessment Center, Universidad de Chile, Santiago, Chile. mkato@uchile.cl
Araki
Akihiro
A
Ogura
Takahiro
T
Valdivia
Ruby P A
RP
D000225:Adenine / Q000031:analogs & derivatives* / Q000633:toxicity*; D000818:Animals; D019976:Cloning, Organism; D004305:Dose-Response Relationship, Drug; D009153:Mutagens / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000187:drug effects*
D009153:Mutagens; C001020:N(6),N(6)-dimethyladenine; D000225:Adenine