The effect of the active component of Dendrostellera lessertii on the adhesive property of human platelets and HL-60 cells.
To evaluate the mediation of cell adhesion by Dendrostellera lessertii, an anti-cancer medicinal plant in Iranian folk medicine, the adhesion of thrombin activated human platelets to the cultured monocytes or HL-60 cells was investigated under the effect of the plant extract (0.4 mg/ml) and one of its purified components, 3-hydrgenkwadaphnin (0.2 nM). Treatment of the platelets with the plant extract or/3-hydrgenkwadaphnin, for various time intervals, followed by their activation by thrombin resulted in 80-90% reduction in the number of monocytes with more than 10 attached platelets. Similarly, under almost all identical conditions, the adhesion of the activated platelets to HL-60 cells was decreased by 90%. The adhesion of thrombin activated platelets to the plant extract treated HL-60 cells was also reduced significantly (95%). These data clearly indicates that Dendrostellera lessertii is capable of mediating tumor metastasis through affecting the adhesion properties of the cells.
Yazdanparast
Razieh
R
Institute of Biochemistry and Biophysics, P.O. Box 13145 - 1384 University of Tehran, Tehran, Iran. yazdan@ibb.ut.ac.ir
Mianabadi
Manijeh
M
D000328:Adult; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D018922:HL-60 Cells / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007492:Iran; D008297:Male; D008519:Medicine, Traditional; D009000:Monocytes / Q000187:drug effects / Q000502:physiology; D010936:Plant Extracts / Q000494:pharmacology*; D010973:Platelet Adhesiveness / Q000187:drug effects*; D029645:Thymelaeaceae
D000970:Antineoplastic Agents; D010936:Plant Extracts