title: Nanoporous aluminum oxide affects neutrophil behaviour.
abstract: This study evaluates neutrophil responses on aluminum oxide membranes. Using an in vitro cell culture system, we have found that the pore size (20 and 200 nm in diameter) of alumina membranes have a significant effect on leukocyte morphology and activation. Specifically, our results show that 20-nm pore-size membranes were more potent in triggering PMN spreading and extending of pseudopodia than 200-nm pore-size membranes. The morphological changes are also associated with cell activation. In fact, adherent neutrophils on 20-nm pore-size membranes elicit much stronger initial oxygen free radical production. Overall, our results point out that membrane pore size significantly affects the extent of cellular responses of adherent neutrophils.
authors.affiliation: Department of Surface Biotechnology, BMC, Husarg. 3, D7:3, Box 577, 751 23 Uppsala, Sweden. Marjam.Karlsson@ytbioteknik.uu.se
authors.forename: M
authors.initials: M
authors.lastname: Karlsson
authors.forename: A
authors.initials: A
authors.lastname: Johansson
authors.forename: L
authors.initials: L
authors.lastname: Tang
authors.forename: M
authors.initials: M
authors.lastname: Boman
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000494:pharmacology*; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002478:Cells, Cultured; D006801:Humans; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D018375:Neutrophil Activation / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D000537:Aluminum Oxide