Sodium butyrate suppresses interferon-gamma-, but not lipopolysaccharide-mediated induction of nitric oxide and tumor necrosis factor-alpha in microglia.
In the present study, we demonstrate that sodium butyrate repressed IFN-gamma-induced expression of iNOS and TNF-alpha, but had little effect on LPS-induced expression in BV2 murine microglial cells. Sodium butyrate significantly inhibited NF-kappa B binding and NF-kappa B-mediated transcription induced by IFN-gamma, suggesting that the anti-inflammatory effect of sodium butyrate is mediated via specific inhibition of the NF-kappa B pathway. IFN-gamma is a major stimulator of innate and adaptive immune response. Thus, the specific down-regulation of IFN-gamma-induced microglial activation by sodium butyrate may provide potential therapeutic strategies for a variety of inflammatory diseases in the central nervous system.
Kim
Hee-Sun
HS
Department of Neuroscience, Ewha Institute of Neuroscience and Medical Research Center, College of Medicine, Ewha Woman's University, Seoul, South Korea. hskimp@mm.ewha.ac.kr
Whang
So-Young
SY
Woo
Moon-Sook
MS
Park
Jin-Sun
JS
Kim
Won-Ki
WK
Han
Inn-Oc
IO
D000818:Animals; D015153:Blotting, Western; D020148:Butyric Acid / Q000494:pharmacology*; D002478:Cells, Cultured; D024202:Electrophoretic Mobility Shift Assay; D004797:Enzyme-Linked Immunosorbent Assay; D007371:Interferon-gamma / Q000187:drug effects* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism; D016328:NF-kappa B / Q000187:drug effects / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis / Q000187:drug effects; D052247:Nitric Oxide Synthase Type II; D011994:Recombinant Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000276:immunology; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000378:metabolism*
D008070:Lipopolysaccharides; D016328:NF-kappa B; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D020148:Butyric Acid; D009569:Nitric Oxide; D007371:Interferon-gamma; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse