Polyamines reduces lipid peroxidation induced by different pro-oxidant agents.
Polyamines, among other functions, are considered to act as a free radical scavenger and antioxidant. The quinolinic acid (QA), sodium nitroprusside (SNP) and iron (Fe+2) stimulate production of free radicals and lipid peroxidation. In the present study, we investigated the free radical and/or aldehyde scavenger effects of polyamines spermine and spermidine on thiobarbituric acid reactive species (TBARS) production induced by QA, SNP, Fe+2/EDTA system and free Fe2+ in rat brain. Spermine and spermidine inhibited QA-induced TBARS production; however spermine was a better antioxidant than spermidine. Spermine also inhibited SNP-, Fe+2/EDTA- and free Fe2+-induced TBARS production, but had a modest effect. Spermidine, in turn, also discretely inhibited SNP-, Fe+2/EDTA- and free Fe2+-induced TBARS production. In the presence of MK-801, QA-induced TBARS production was considerably more inhibited by polyamines. In addition, arcaine does not affect the reducer effect of polyamines. The present findings suggest that the observed effects of polyamines are not related to the activation of NMDA receptor but with their antioxidant and free radical scavenger properties.
Bellé
Nádia Aléssio Velloso
NA
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus UFSM, Santa Maria, RS 97105-900, Brazil.
Dalmolin
Gerusa Duarte
GD
Fonini
Graciela
G
Rubin
Maribel Antonello
MA
Rocha
João Batista Teixeira
JB
D000818:Animals; D000975:Antioxidants; D001645:Biguanides / Q000494:pharmacology; D015317:Biogenic Polyamines / Q000494:pharmacology*; D016291:Dizocilpine Maleate / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D007501:Iron / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects*; D009599:Nitroprusside / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology*; D017378:Quinolinic Acid / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D001645:Biguanides; D015317:Biogenic Polyamines; D016877:Oxidants; D017392:Thiobarbituric Acid Reactive Substances; D009599:Nitroprusside; D013096:Spermine; C006624:arcaine; D016291:Dizocilpine Maleate; D004492:Edetic Acid; D007501:Iron; D017378:Quinolinic Acid; D013095:Spermidine