Structural organization of the murine microsomal glutathione S-transferase gene (MGST1) from the 129/SvJ strain: identification of the promoter region and a comprehensive examination of tissue expression.
The structure and regulation of the murine microsomal glutathione transferase gene (MGST1) from the 129/SvJ strain is described and demonstrates considerable difference in nucleotide sequence and consequently in restriction enzyme sites as compared to other mouse strains. A comparison of the amino acid sequence for MGST1 revealed one difference in exon 2 between the 129/SvJ strain (arginine at position 5) and the sequence previously reported for the Balb/c strain (lysine). The promoter region immediately upstream of the dominant first exon is functional, transcriptionally responds to oxidative stress, and is highly homologous to the human region. Oxidative stress also induced the production of endogenous MGST1 mRNA. The tissue-specific expression of MGST1 mRNA was studied, and as anticipated, was indeed highest in liver. There was, however, marked mRNA expression in several tissues not previously studied including smooth muscle, epidymus, ovaries, and endocrine glands in which the expression of various peroxidases is also very high (salivary and thyroid). Overall, there was a good agreement between the mRNA content detected and previous reports of MGST1 activity with the exception of brain tissue.
Kelner
Michael J
MJ
Department of Pathology, University of California, 8320 UCSD Medical Center, 200 West Arbor Drive, San Diego, CA, USA. mkelner@ucsd.edu
Bagnell
Richard D
RD
Morgenstern
Ralf
R
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D015152:Blotting, Northern; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D005091:Exons; D005822:Genetic Vectors; D005982:Glutathione Transferase / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013329:Structure-Activity Relationship; D014018:Tissue Distribution; D014162:Transfection; D001616:beta-Galactosidase / Q000378:metabolism
D012333:RNA, Messenger; D008156:Luciferases; C122107:microsomal glutathione S-transferase-I; D005982:Glutathione Transferase; D001616:beta-Galactosidase