Comparison of IL-6-induced STAT1 activation in different responsive cells.
OBJECTIVE
To investigate the activation status of signal transducer and activator of transcription 1 (STAT1) induced by interlerkin-6 (IL-6) in different responsive cells.


METHODS
Immunoblot analysis was performed with antibodies against STAT1 and tyrosine-phosphorylated STAT1.


RESULTS
It's found that IL-6 had growth-promoting effects on 7TD1 and TF1 cells but had little effect on the tyrosine-phosphorylation of STAT1. On the other hand, IL-6 activated STAT1 in a dose and time-dependent manner in M1, R2 and U937 cells which underwent growth arrest and macrophage differentiation upon IL-6 induction.


CONCLUSIONS
These results suggest that IL-6-induced STAT1 activation might be associated with growth-inhibitory effects, and our data might help to explain why IL-6 exerts distinct biological effects in different target cells.
Zhang
Ji-Yan
JY
Department of Molecular Immunology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850,China.
Li
Yan
Y
Ren
Yun-Fang
YF
Shen
Bei-Fen
BF
D004268:DNA-Binding Proteins / Q000378:metabolism; D006801:Humans; D010766:Phosphorylation; D050794:STAT1 Transcription Factor; D015398:Signal Transduction / Q000187:drug effects; D015534:Trans-Activators / Q000378:metabolism; D014443:Tyrosine
D004268:DNA-Binding Proteins; D050794:STAT1 Transcription Factor; D015534:Trans-Activators; D014443:Tyrosine