Influence of origin of isolates, especially endocarditis isolates, and various genes on biofilm formation by Enterococcus faecalis.
Endocarditis isolates of Enterococcus faecalis produced biofilm significantly more often than nonendocarditis isolates, and 39% of 79 versus 6% of 84 isolates produced strong biofilm (P < 0.0001). esp was not required, but its presence was associated with higher amounts of biofilm (P < 0.001). Mutants disrupted in dltA, efaA, ace, lsa, and six two-component regulatory systems were largely unaltered, while disruptions in epa (encoding enterococcal polysaccharide antigen), atn (encoding an autolysin), gelE (encoding gelatinase), and fsr (encoding the E. faecalis regulator) [corrected] resulted in fewer attached bacteria, as determined using phase-contrast microscopy, and less biofilm (P < 0.0001).
Mohamed
Jamal A
JA
Division of Infectious Diseases, Department of Internal Medicine, University of Texas Medical School, Houston, Texas 77030, USA.
Huang
Wenxiang
W
Nallapareddy
Sreedhar R
SR
Teng
Fang
F
Murray
Barbara E
BE
D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms; D004697:Endocarditis, Bacterial / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D018093:Gelatinases / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D009154:Mutation; D011137:Polystyrenes
D001426:Bacterial Proteins; D008565:Membrane Proteins; D011137:Polystyrenes; C116707:enterococcal surface protein, esp; D018093:Gelatinases