Generic lanthanide fluoroimmunoassay for the simultaneous screening of 18 sulfonamides using an engineered antibody.
Sulfa antibiotics (sulfonamides) are used in veterinary and human medicine for therapeutic and prophylactic purposes. Veterinary use can result in foodstuffs derived from animals being contaminated with residual sulfonamides. Current sulfonamide-screening methods (mainly based on bacterial growth inhibition) are slow and inaccurate, since sensitivities of bacteria to different sulfonamides vary a lot. Therefore, a rapid immunoassay that was able to detect at least 18 different sulfonamides at the MRL level (100 microg/kg) from food samples in a single reaction was developed. The assay was reproducible and adequately accurate for screening purposes. The presence of sulfonamide metabolites did not cause major assay interference. We also demonstrated reliable detection of sulfonamides from a panel of meat, milk, and serum samples with the assay.
Korpimäki
Teemu
T
Department of Biotechnology, University of Turku, Turku, Finland. teemu.korpimaki@utu.fi
Hagren
Virve
V
Brockmann
Eeva-Christine
EC
Tuomola
Mika
M
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D004353:Drug Evaluation, Preclinical; D015200:Fluoroimmunoassay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics* / Q000276:immunology; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D008460:Meat / Q000032:analysis; D008892:Milk / Q000737:chemistry; D015202:Protein Engineering; D013449:Sulfonamides / Q000032:analysis* / Q000097:blood / Q000737:chemistry
D000900:Anti-Bacterial Agents; D007135:Immunoglobulin Variable Region; D028581:Lanthanoid Series Elements; D013449:Sulfonamides