title: Insulin-induced changes of proteolytic activity of the lysosomal enzymes.
abstract: OBJECTIVE
  Changes in the activity of alanine aminopeptidase, leucine aminopeptidase and cathepsins D and L in the liver and kidney of male and female of mice, injected with 0.4 IU/kg b.w. insulin for 4 and 8 days.
  
  
  METHODS
  The homogenates of the liver and kidney were taken for examination. The activity of alanine aminopeptidase, leucine aminopeptidase and cathepsins D and L has been determined according to [1] method.
  
  
  RESULTS
  The activity of alanine aminopeptidase, leucine aminopeptidase, cathepsins D and L in the liver and kidney of male and female of mice decreased in effect of insulin injections for 4 and 8 days.
  
  
  CONCLUSIONS
  The changes of enzyme activities showed a stimulating effect of the insulin injection on the labilization of lysosomal membranes. The range of the reaction remained in a relationship with the kind of the organ, the type of enzyme, time over which insulin introduced operates in the organism, and with the sex.
authors.affiliation: Department of Genetics, Institute of Biology, Swietokrzyska Academy, Poland. b.witek@pu.kielce.pl
authors.forename: Bozena
authors.initials: B
authors.lastname: Witek
authors.forename: Ewa
authors.initials: E
authors.lastname: Ochwanowska
authors.forename: Adam
authors.initials: A
authors.lastname: Kolataj
authors.forename: Teodora
authors.initials: T
authors.lastname: Król
authors.forename: Danuta
authors.initials: D
authors.lastname: Baranowska
authors.forename: Jan
authors.initials: J
authors.lastname: Rafay
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D018826:CD13 Antigens / Q000378:metabolism; D002402:Cathepsin D / Q000378:metabolism; D056668:Cathepsin L; D002403:Cathepsins / Q000378:metabolism; D003546:Cysteine Endopeptidases; D015536:Down-Regulation; D005260:Female; D007328:Insulin / Q000502:physiology*; D007668:Kidney / Q000201:enzymology; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D051379:Mice; D010447:Peptide Hydrolases / Q000378:metabolism*; D011897:Random Allocation
chemical_list: D001786:Blood Glucose; D007328:Insulin; D002403:Cathepsins; D010447:Peptide Hydrolases; D007931:Leucyl Aminopeptidase; D018826:CD13 Antigens; D003546:Cysteine Endopeptidases; D056668:Cathepsin L; C534278:Ctsl protein, mouse; D002402:Cathepsin D