Mapping of domains responsible for nucleocapsid protein-phosphoprotein interaction of Henipaviruses.
Hendra virus (HeV) and Nipah virus (NiV) are members of a new genus, Henipavirus, in the family paramyxoviridae. Each virus encodes a phosphoprotein (P) that is significantly larger than its counterparts in other known paramyxoviruses. The interaction of this unusually large P with its nucleocapsid protein (N) was investigated in this study by using recombinant full-length and truncated proteins expressed in bacteria and a modified protein-blotting protein-overlay assay. Results from our group demonstrated that the N and P of both viruses were able to form not only homologous, but also heterologous, N-P complexes, i.e. HeV N was able to interact with NiV P and vice versa. Deletion analysis of the N and P revealed that there were at least two independent N-binding sites on P and they resided at the N and C termini, respectively. Similarly, more than one P-binding site was present on N and one of these was mapped to a 29 amino acid (aa) C-terminal region, which on its own was sufficient to interact with the extreme C-terminal 165 aa region of P.
Chan
Y P
YP
Institute of Biological Sciences (Genetics), University of Malaya, 50603 Kuala Lumpur, Malaysia.
Koh
C L
CL
Institute of Biological Sciences (Genetics), University of Malaya, 50603 Kuala Lumpur, Malaysia.
Lam
S K
SK
Department of Medical Microbiology, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Wang
L-F
LF
CSIRO Livestock Industries, Australian Animal Health Laboratory, PO Bag 24, Geelong, Victoria 3220, Australia.
D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000235:genetics; D045403:Henipavirus / Q000737:chemistry*; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000737:chemistry*; D010750:Phosphoproteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D014764:Viral Proteins / Q000737:chemistry*
D019590:Nucleocapsid Proteins; D010750:Phosphoproteins; D011994:Recombinant Proteins; D014764:Viral Proteins; C110268:nonstructural protein C;nonstructural protein V;phosphoprotein P, Hendra virus