[Interaction between p53 and HSP70 in human hepatocellular carcinoma tissues].
OBJECTIVE
To investigate the interaction between p53 and heat shock protein70 (HSP70) in human hepatocellular carcinoma tissues.


METHODS
HSP70 and p53-double positive samples were screened by imunohistochemical staining from twelve human hepatocellular carcinoma tissues, and the tissue homogenate was prepared. Coimmunoprecipitation was performed by using either anti-HSP70 mAb or anti-p53 mAb. The immunoprecipitate was analysed by SDS-PAGE and Western blot.


RESULTS
Immunohistochemical staining detection showed that three samples out of twelve cases were double positive for both HSP70 and p53. p53 protein existed in the immunoprecipitate of anti-HSP70 mAb, while there was HSP70 protein in the immunoprecipitate of anti-p53 mAb.


CONCLUSIONS
HSP70 and p53 exist as a complex in human hepatocellular carcinoma tissues, which will provide a new route for studying the pathogenesis and immunotherapy of human hepatocellular carcinoma.
Cui
Chong-Wei
CW
Department of Pathology, Fourth Military Medical University, Xi'an 710032, China.
Yang
Shou-Jing
SJ
Liu
Yan-Ping
YP
Liu
Yan-Fang
YF
D006528:Carcinoma, Hepatocellular; D004591:Electrophoresis, Polyacrylamide Gel; D018840:HSP70 Heat-Shock Proteins; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D018840:HSP70 Heat-Shock Proteins; D016159:Tumor Suppressor Protein p53