Electrochemical measurements confirm the preferential bonding of the antimetastatic complex [ImH][RuCl(4)(DMSO)(Im)] (NAMI-A) with proteins and the weak interaction with nucleobases.
An electrochemical and biological study of interaction between the prototypical antimetastatic drug imidazolium trans-tetrachlorodimethylsulfoxideimidazoleruthenate (III) complex, [ImH][RuCl(4)(DMSO)(Im)] (DMSO = dimethylsulfoxide, Im = imidazole), nicknamed NAMI-A, and several biomolecules, namely DNA, bovine (BSA) and human (HSA) serum albumin, is reported. Electrochemistry offers great advantages over the existing devices based on optical techniques, since it provides rapid, simple, and low-cost information whether the interaction occurs or not. Moreover, we describe some biochemical assays to test the interaction of NAMI-A with ribonucleoprotein telomerase and protein Taq polymerase. All the data confirm the preferential interaction of NAMI-A with proteins with respect to nucleotides, especially when compared with the behaviour of the well-known alkylating drug cisplatin in the presence of the same targets.
Ravera
Mauro
M
Dipartimento Scienze dell'Ambiente e della Vita, Università del Piemonte Orientale Amedeo Avogadro, Spalto Marengo 33, 15100 Alessandria, Italy.
Baracco
Sara
S
Cassino
Claudio
C
Colangelo
Donato
D
Bagni
Graziana
G
Sava
Gianni
G
Osella
Domenico
D
D018906:Antineoplastic Agents, Alkylating / Q000737:chemistry*; D004121:Dimethyl Sulfoxide / Q000031:analogs & derivatives* / Q000737:chemistry*; D004563:Electrochemistry; D008968:Molecular Conformation; D015394:Molecular Structure; D009711:Nucleotides / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D017975:Ruthenium Compounds; D019914:Taq Polymerase / Q000737:chemistry*; D019098:Telomerase / Q000737:chemistry*
D018906:Antineoplastic Agents, Alkylating; D009711:Nucleotides; D009942:Organometallic Compounds; D017975:Ruthenium Compounds; C113661:imidazolium-bis(imidazole)dimethylsulfoxideimidazotetrachlororuthenate(III); D019914:Taq Polymerase; D019098:Telomerase; D004121:Dimethyl Sulfoxide