Development of the mammary gland requires DGAT1 expression in stromal and epithelial tissues.
Mammary gland development is a complex process that is dependent on interactions between the developing mammary epithelium and the surrounding stromal tissues. We show that mice lacking the triglyceride synthesis enzyme acyl CoA:diacylglycerol transferase 1 (DGAT1) have impaired mammary gland development, characterized by decreased epithelial proliferation and alveolar development, and reduced expression of markers of functional differentiation. Transplantation studies demonstrate that the impaired development results from a deficiency of DGAT1 in both the stromal and epithelial tissues. Our findings are the first to link defects in stromal lipid metabolism to impaired mammary gland development.
Cases
Sylvaine
S
Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94141-1900, USA.
Zhou
Ping
P
Shillingford
Jonathan M
JM
Wiseman
Bryony S
BS
Fish
Jo Dee
JD
Angle
Christina S
CS
Hennighausen
Lothar
L
Werb
Zena
Z
Farese
Robert V
RV
D000217:Acyltransferases / Q000096:biosynthesis* / Q000502:physiology*; D000818:Animals; D017209:Apoptosis; D002454:Cell Differentiation; D051048:Diacylglycerol O-Acyltransferase; D004847:Epithelial Cells / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D015151:Immunoblotting; D007150:Immunohistochemistry; D007774:Lactation; D050356:Lipid Metabolism; D008321:Mammary Glands, Animal / Q000196:embryology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011233:Precipitin Tests; D012313:RNA / Q000378:metabolism; D017154:Stromal Cells / Q000378:metabolism*; D013997:Time Factors; D016378:Tissue Transplantation
D012313:RNA; D000217:Acyltransferases; C494882:Dgat1 protein, mouse; D051048:Diacylglycerol O-Acyltransferase