Isolation and characterization of a novel angiotensin I-converting enzyme inhibitory peptide derived from the edible mushroom Tricholoma giganteum.
The fruiting body of Tricholoma giganteum has many pharmaceutical uses and has long been utilized as a home remedy in Asia. This study describes the extraction and characterization of the first angiotensin I-converting enzyme (ACE) inhibitory peptide from T. giganteum. The maximum ACE inhibitory activity (IC50: 0.31 mg) was obtained when the fruiting body of T. giganteum was extracted with distilled water at 30 degrees C for 3 h. After the purification of ACE inhibitory peptides with ultrafiltration, Sephadex G-25 column chromatography, and reverse-phase HPLC, an active fraction with an IC50 of 0.04 mg and a yield of 0.3% was obtained. The ACE inhibitory peptide was a novel tripeptide, showing very low similarity to other ACE inhibitory peptide sequences, and was sequenced as Gly-Glu-Pro. The purified ACE inhibitor from T. giganteum competitively inhibited ACE, and it maintained inhibitory activity even after incubation with proteases. ACE inhibitor from T. giganteum showed a clear antihypertensive effect in spontaneously hypertensive rats (SHR), at a dosage of 1 mg/kg.
Hyoung Lee
Dae
D
Department of Genetic Engineering, Paichai University, Daejeon 302-735, Republic of Korea.
Ho Kim
Jae
J
Sik Park
Jeong
J
Jun Choi
Young
Y
Soo Lee
Jong
J
D000363:Agaricales / Q000737:chemistry* / Q000254:growth & development; D000818:Animals; D002853:Chromatography, Liquid; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification*; D005656:Fungal Proteins / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification*; D006973:Hypertension / Q000188:drug therapy; D009842:Oligopeptides / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification*; D007703:Peptidyl-Dipeptidase A / Q000737:chemistry*; D011817:Rabbits; D051381:Rats; D011918:Rats, Inbred SHR
D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D009842:Oligopeptides; D007703:Peptidyl-Dipeptidase A