title: Association of single nucleotide polymorphisms within cytokine genes with juvenile idiopathic arthritis in the Czech population.
abstract: OBJECTIVE
  To examine the possible association of juvenile idiopathic arthritis (JIA) with polymorphisms within cytokine genes in the Czech population.
  
  
  METHODS
  In a case-control study, genotypes of 130 patients with JIA (63 male, 67 female; age at onset 7.6 +/- 4.4 yrs; 43 oligoarticular, 72 polyarticular, 15 systemic form) were compared to 102 healthy unrelated blood donors. Using the polymerase chain reaction technique with sequence-specific primers from the 13th IHWG workshop, we analyzed 19 single nucleotide polymorphisms within 12 different cytokine genes [interleukin (IL)-1a, IL-1beta, IL-2, IL-4, IL-6, IL-10, IL-12, transforming growth factor (TGF)-beta, interferon (IFN)-g], and related molecules (IL-1R, IL-1RA, IL-4Ra). Genotype frequencies were compared using chi-square analysis, and the significance level was corrected for the number of independent tests.
  
  
  RESULTS
  Significant positive association was found for the G allele of the IL-4 -1098 T/G polymorphism, which was carried by 10% of cases and 25% of controls [odds ratio (OR) 0.32, 95% confidence interval (CI) 0.16-0.67, corrected p = 0.038). Also, a nonsignificant increase in the frequency of the IL-1beta +3962 C allele was detected in cases (96%) versus controls (84%) (OR 4.65, 95% CI 1.64-13.2, corrected p = 0.091). We did not replicate previously found associations with the IL-1a, IL-6, IL-10, and IL-1RA polymorphisms.
  
  
  CONCLUSIONS
  Our study showed association with JIA for the IL-4 -1098 T/G polymorphism. It also underlines the genetic contribution of IL-1 polymorphisms to the pathogenesis of JIA, as another polymorphism within the IL-1beta may influence the risk of the disease.
authors.affiliation: Department of Paediatrics, Motol University Hospital, Prague, Czech Republic. Ondrej.Cinek@Lfmotol.cuni.cz
authors.forename: Ondrej
authors.initials: O
authors.lastname: Cinek
authors.forename: Pavla
authors.initials: P
authors.lastname: Vavrincová
authors.forename: Ilja
authors.initials: I
authors.lastname: Striz
authors.forename: Pavel
authors.initials: P
authors.lastname: Drevínek
authors.forename: Pavlína
authors.initials: P
authors.lastname: Sedláková
authors.forename: Jan
authors.initials: J
authors.lastname: Vavrinec
authors.forename: Antonij
authors.initials: A
authors.lastname: Slavcev
mesh_terms: D000293:Adolescent; D000328:Adult; D001171:Arthritis, Juvenile / Q000235:genetics*; D002648:Child; D016207:Cytokines / Q000235:genetics*; D018153:Czech Republic; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: D016207:Cytokines