Headspace solid-phase microextraction and capillary gas chromatographic-mass spectrometric determination of rivastigmine in canine plasma samples.
A simple, rapid and sensitive method for determination of rivastigmine in plasma samples was developed using headspace solid-phase microextraction (HS-SPME) and gas chromatography with mass spectrometry (GC-MS). The optimum conditions for the SPME procedure were: headspace extraction on a 65-microm polydimethylsiloxane/divinylbenzene (PDMS/DVB) fiber; 0.5 ml of plasma modified with 1.0 ml of sodium hydroxide-sodium carbonate solution (0.7 M:0.5M); extraction temperature of 100 degrees C, with stirring at 2000 rpm for 30 min. The calibration curve showed linearity in the range from 0.2 to 80 ng/ml with regression coefficient corresponding to 0.9965 and coefficient of the variation of the points of the calibration curve lower than 10%. The quantification limit for rivastigmine in plasma was 0.2 ng/ml. The method was applied to determination of rivastigmine in canine plasma samples from animals after a single oral administration.
Sha
Yunfei
Y
Department of Pharmacy, Fudan University, Shanghai 200032, PR China.
Deng
Chunhui
C
Liu
Zhen
Z
Huang
Taomin
T
Yang
Bei
B
Duan
Gengli
G
D000818:Animals; D002800:Cholinesterase Inhibitors / Q000097:blood*; D004285:Dogs; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D018696:Neuroprotective Agents / Q000097:blood*; D048448:Phenylcarbamates / Q000097:blood*; D000068836:Rivastigmine
D002800:Cholinesterase Inhibitors; D018696:Neuroprotective Agents; D048448:Phenylcarbamates; D000068836:Rivastigmine