Allowable warm ischemic time to tracheal extraction for allotransplantation of cryopreserved trachea.
OBJECTIVE
The allowable warm ischemic time from circulatory arrest to tracheal extraction for allotransplantation of cryopreserved tracheal grafts from cadaveric donors was examined in adult mongrel dogs.


METHODS
The animals were divided into 4 groups (n = 28) according to the warm ischemic time of less than 1 hour, 3 hours, 6 hours, and 12 hours, after transplantation, and comparisons were made. The grafts were cryopreserved for at least 2 months and were evaluated by extraction from the recipients generally 2 months after transplantation.


RESULTS
All the grafts with a warm ischemic time of less than 1 hour were viable and did not show stenosis. This group did not differ significantly from the groups with a warm ischemic time of 3 and 6 hours in terms of viability. However, all of the grafts with a warm ischemic time of 12 hours showed stenosis, and there was a significantly lower viability rate. Histological examination of the grafts showed that warm ischemia caused necrosis of the tracheal cartilage.


CONCLUSIONS
Based on these results, it was concluded that 6 hours was the maximum allowable warm ischemic time for cryopreserved tracheal transplantation, and that necrosis of the tracheal cartilage due to warm ischemia reduced the viability of the grafts.
Kitamura
Yasuhiro
Y
Department of Cancer and Thoracic Surgery, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata, Okayama 700-8558, Japan.
Shimizu
Nobuyoshi
N
Aoe
Motoi
M
Date
Hiroshi
H
Nagahiro
Itaru
I
Andou
Akio
A
D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D004285:Dogs; D006085:Graft Survival; D009336:Necrosis; D013696:Temperature; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*; D014132:Trachea / Q000473:pathology / Q000601:surgery / Q000637:transplantation*; D014184:Transplantation, Homologous