Alterations in chemokine expression following Theiler's virus infection and restraint stress.
Restraint stress (RS) applied to mice during acute infection with Theiler's virus causes corticosterone-induced immunosuppression. This effect was further investigated by measuring chemokine changes in the spleen and central nervous system (CNS) using an RNase Protection Assay. mRNAs for lymphotactin (Ltn), interferon-induced protein-10 (IP-10), MIP-1 beta, monocyte chemoattractant protein-1 (MCP-1) and TCA-3 were detected in the spleen at day 2 pi, but not in the brain of CBA mice infected with Theiler's virus. Ltn, IP-10 and RANTES were elevated in both the spleen and the brain at day 7 pi, and were significantly decreased by RS in the brain. RS also resulted in decreased inflammation within the CNS.
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Department of Veterinary Anatomy and Public Health, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843, USA.
Belyavskyi
M
M
Johnson
R R
RR
Sieve
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AN
Storts
R
R
Meagher
M W
MW
Welsh
C J R
CJ
D000818:Animals; D001921:Brain / Q000276:immunology* / Q000473:pathology / Q000821:virology; D018188:Cardiovirus Infections / Q000276:immunology* / Q000503:physiopathology; D018925:Chemokines / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis; D012149:Restraint, Physical; D013154:Spleen / Q000276:immunology / Q000473:pathology / Q000821:virology; D013315:Stress, Psychological / Q000276:immunology*; D017945:Theilovirus / Q000378:metabolism*; D013950:Thymus Gland / Q000276:immunology / Q000473:pathology / Q000821:virology
D018925:Chemokines; D012333:RNA, Messenger