Evaluation of a prototype HCV NS5b assay for typing strains of hepatitis C virus isolated from Tunisian haemodialysis patients.
Hepatitis C virus (HCV) strains isolated from 68 haemodialysis Tunisian patients exhibiting chronic infection were genotyped targeting the NS5b region of the HCV genome using a prototype assay developed by Bayer HealthCare-Diagnostics (TRUGENE NS5b HCV). The overall results were compared to those obtained with another assay of the same company based on sequencing of the 5' non-coding region (TRUGENE HCV 5'NC genotyping kit). All strains could be typed by the 5'NC typing kit, but only 62 (91; 2%) by the NS5b prototype assay. All the 62 strains typed by both methods exhibited the same pattern at the type level: 57 were type 1, 3 were type 2, and 2 were type 4. At the subtype level, eight strains that gave undetermined results by the 5'NC kit were successfully typed by the NS5b kit; eight additional strains exhibited discrepant results. The overall agreement between the two assays was 74.2% at the subtype level. In conclusion, the NS5b region appears to be much more accurate than the 5'NC region to subtype HCV strains, especially in those isolated from patients attending haemodialysis centres where the subtype distribution suggests frequent nosocomial transmissions.
Othman
S Ben
SB
Laboratoire des Maladies Transmissibles, Department of Microbiology, Faculty of Pharmacy, Monastir, Hôpital Farhat Hached, Sousse, Tunisia.
Trabelsi
A
A
Monnet
A
A
Bouzgarrou
N
N
Grattard
F
F
Beyou
A
A
Bourlet
T
T
Pozzetto
B
B
D020121:5' Untranslated Regions / Q000032:analysis* / Q000737:chemistry; D005069:Evaluation Studies as Topic; D005838:Genotype; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics; D006801:Humans; D011933:Reagent Kits, Diagnostic; D014416:Tunisia; D017361:Viral Nonstructural Proteins / Q000032:analysis* / Q000737:chemistry; D012324:RNA-Dependent RNA Polymerase
D020121:5' Untranslated Regions; D011933:Reagent Kits, Diagnostic; D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase