[Application of vital dye CFDA-SE to study lymphocytic proliferation].
OBJECTIVE
To explore the application value of vital dye CFDA-SE to study of lymphocytic proliferation.


METHODS
CFDA-SE staining, fluorescence antibody labeling, flow cytometry and related software were used to detect the fluorescence intensity and analysis the proliferation kinetics of lymphocytes and their subsets after stimulation with polyclonal stimulators.


RESULTS
Lymphocytes divided after stimulation of PDB+ionomycin or ConA for 48 h, manifesting the serial halving of fluorescence intensity. CsA inhibited the proliferative effect of ConA on lymphocytes and no CFSE fluorescence halving were seen. Proliferation of CD4(+) T cells and CD8(+) T cells were asynchronous after ConA stimulation for 48 h, which became more obvious at the time of 72 h. Proliferation-related indexs got ten from ModFit(TM) software showed that the proliferative effect of ConA on CD8(+) T cells was stronger than that on CD4(+) T cells.


CONCLUSIONS
CFDA-SE staining combined with fluorescent antibody labeling and cytometry were powerful tools for analysis of lymphocytic proliferation.
Zhao
Jing-Xian
JX
Key Laborotary of Ministry of Education for Tissue Transplantation and Immunology, Jinan University, Guangzhou 510632,China.
Zeng
Yao-Ying
YY
He
Xian-Hui
XH
Wang
Nan
N
Di
Jing-Fang
JF
Zeng
Shan
S
D000818:Animals; D018414:CD8-Positive T-Lymphocytes; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry; D008213:Lymphocyte Activation / Q000187:drug effects; D008807:Mice, Inbred BALB C