title: Expression pattern of the novel gene EG-1 in cancer.
abstract: OBJECTIVE
  We recently discovered a novel gene responsive to tumor-conditioned media: endothelial-derived gene 1 (EG-1). Its transcript has been shown to be present in epithelial cells, as well as in endothelial cells. In this study, we examined the levels of EG-1 protein expression in breast, colon, prostate, and lung cancers, which constitute the four most common solid malignancies in the United States.
  
  
  METHODS
  Polyclonal antibodies were generated that recognize the EG-1 peptide. These antibodies were used in immunoblot analysis, as well as immunohistochemistry of multiple human clinical specimens of cancer.
  
  
  RESULTS
  In immunoblots of whole cell lysates, EG-1 antibodies revealed the presence of a 22-kDa peptide. Immunohistochemistry of breast, colon, and prostate specimens showed higher levels of EG-1 peptides in cancer tissues, in comparison with their benign counterparts. However, EG-1 expression was minimal in both benign and malignant lung tissues.
  
  
  CONCLUSIONS
  Here, we demonstrated that the expression of EG-1 is elevated in cancerous in comparison to benign epithelial cells, as seen in immunohistochemistry of human pathological specimens. These observations collectively support the hypothesis that the novel gene EG-1 is associated with the malignant phenotype of the common epithelial-derived cancers of the breast, colon, and prostate.
authors.affiliation: Department of Surgery, Division of Oncology, University of California at Los Angeles, Los Angeles, California 90095, USA.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhang
authors.forename: Raymond Scott
authors.initials: RS
authors.lastname: Maul
authors.forename: JianYu
authors.initials: J
authors.lastname: Rao
authors.forename: Sophia
authors.initials: S
authors.lastname: Apple
authors.forename: David
authors.initials: D
authors.lastname: Seligson
authors.forename: Maryam
authors.initials: M
authors.lastname: Sartippour
authors.forename: Rosalio
authors.initials: R
authors.lastname: Rubio
authors.forename: Mai N
authors.initials: MN
authors.lastname: Brooks
mesh_terms: D000906:Antibodies / Q000737:chemistry; D015153:Blotting, Western; D002460:Cell Line; D045744:Cell Line, Tumor; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D056892:Mediator Complex; D018613:Microscopy, Confocal; D009369:Neoplasms / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry; D010641:Phenotype; D011506:Proteins / Q000378:metabolism*; D014162:Transfection
chemical_list: D000906:Antibodies; C496877:MED28 protein, human; D056892:Mediator Complex; D010455:Peptides; D011506:Proteins