Identification of interferon-tau isoforms expressed by the peri-implantation goat (Capra hircus) conceptus.
Interferon-tau (IFN-tau) is the maternal recognition of pregnancy factor in pecoran ruminants. The aims of this study were to identify the various IFN-tau transcripts in the peri-implantation caprine (ca) conceptus and to compare these nucleotide sequences phylogenetically with established mRNA sequences from the goat. Conceptuses (n = 5) were collected from Boer crossbred and Angora female goats by laparotomy at days 17 and 18 of pregnancy. Total cellular RNA was extracted and RT-PCR was performed by standard procedures using a DNA polymerase with proofreading activity and gene-specific primers complimentary to non-coding regions of the published caIFN-tau sequence. Nine distinct nucleotide sequences were isolated that encode five distinct caIFN-tau proteins. These caIFN-tau have greater sequence homology with ovine IFN-tau (94-96% nucleotide identity; 90-93% amino acid identity) than with bovine IFN-tau (<92% nucleotide identity; <85% amino acid identity). The novel caIFN-tau isoforms contained pronounced nucleotide and amino acid sequence identity with one another (97-99% nucleotide identity; 94-99% amino acid identity) but only moderate sequence identity with the previously identified caIFN-tau (94-96% nucleotide identity; 87-90% amino acid identity). In conclusion, multiple caIFN-tau mRNA species are expressed during peri-implantation conceptus development and distinct clusters of caIFN-tau genes appear to have evolved in this species.
Ealy
Alan D
AD
Department of Dairy & Animal Science, The Pennsylvania State University, University Park, PA 16802, USA. ade3@psu.edu
Wagner
Sara K
SK
Sheils
Ann E
AE
Whitley
Niki C
NC
Kiesling
David O
DO
Johnson
Sally E
SE
Barbato
Guy F
GF
D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary / Q000032:analysis; D010064:Embryo Implantation / Q000235:genetics / Q000502:physiology*; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006041:Goats / Q000196:embryology*; D007370:Interferon Type I / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms / Q000145:classification / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017385:Sequence Homology
D018076:DNA, Complementary; D007370:Interferon Type I; D011257:Pregnancy Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; C020947:interferon tau