title: Adipose tissue transcriptome by serial analysis of gene expression.
abstract: OBJECTIVE
  To describe the genomic expression profile or transcriptome of adipose tissue using the serial analysis of gene expression method.
  
  
  METHODS
  The serial analysis of gene expression strategy is based on isolation of short sequences (tags), which usually correspond to unique transcripts, and on their concatenation into long DNA molecules, which are then cloned and sequenced. Experiments were performed with mRNA from retroperitoneal adipose tissue of male C57BL6 mice.
  
  
  RESULTS
  We isolated 45,996 tags corresponding to more than 17,000 different genes. Eighty-eight genes were expressed at more than 0.1% of the total population and represented 26% of the mRNA population identified. The most expressed genes were: carbonic anhydrase 3 (1.97%), cytochrome c oxidase (COX) 1 (1.47%), COX2 (1.25%), diazepam binding inhibitor (1.04%), a novel transcript (0.87%), COX3 (0.55%), fatty acid-binding protein 4 (0.55%), and NADH dehydrogenase 4 (0.52%). Other genes known to be expressed in adipose tissue, such as uncoupling protein 2, angiotensinogen, adipsin, and insulin-like growth factor 1, were found at a lower level. Several tags corresponding to novel transcripts were also found.
  
  
  CONCLUSIONS
  To our knowledge, the present results provide for the first time a quantitative description of the transcriptome in adipose tissue.
authors.affiliation: Molecular Endocrinology and Oncology Research Center, Laval Univerisity Medical Center and Laval University, Quebec, Canada.
authors.forename: Carl
authors.initials: C
authors.lastname: Bolduc
authors.forename: Marianne
authors.initials: M
authors.lastname: Larose
authors.forename: Nicolas
authors.initials: N
authors.lastname: Lafond
authors.forename: Mayumi
authors.initials: M
authors.lastname: Yoshioka
authors.forename: Marc-André
authors.initials: MA
authors.lastname: Rodrigue
authors.forename: Jean
authors.initials: J
authors.lastname: Morissette
authors.forename: Claude
authors.initials: C
authors.lastname: Labrie
authors.forename: Vincent
authors.initials: V
authors.lastname: Raymond
authors.forename: Jonny
authors.initials: J
authors.lastname: St-Amand
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D020869:Gene Expression Profiling; D015723:Gene Library; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000235:genetics*; D012187:Retroperitoneal Space
chemical_list: D012333:RNA, Messenger