TcZFP1: a CCCH zinc finger protein of Trypanosoma cruzi that binds poly-C oligoribonucleotides in vitro.
We have identified two zinc finger proteins of Trypanosoma cruzi, the protozoan parasite that causes Chagas disease in humans. These proteins, named tcZFP1 and tcZFP2, share the unusual zinc finger motif (CCCH) found in a diverse range of RNA-binding proteins involved in various aspects of the control of cell homeostasis and differentiation. We report here the functional expression of a recombinant tcZFP1, and the relative affinity and stability of the specific complexes formed between the protein and synthetic oligoribonucleotides containing C-rich sequences.
Mörking
Patrícia A
PA
Instituto de Biologia Molecular do Paraná, Rua Professor Algacyr Munhoz Mader 3775, Curitiba, Paraná 81350-010, Brazil.
Dallagiovanna
Bruno M
BM
Foti
Leonardo
L
Garat
Beatriz
B
Picchi
Gisele F A
GF
Umaki
Adriana C S
AC
Probst
Christian M
CM
Krieger
Marco A
MA
Goldenberg
Samuel
S
Fragoso
Stenio P
SP
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D049452:Green Fluorescent Proteins; D007700:Kinetics; D008164:Luminescent Proteins / Q000737:chemistry; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides / Q000737:chemistry*; D011132:Polyribosomes / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000737:chemistry* / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000378:metabolism*; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D008164:Luminescent Proteins; D009843:Oligoribonucleotides; D015800:Protozoan Proteins; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; C444079:zinc finger protein 1, Trypanosoma brucei; C444080:zinc finger protein 2, Trypanosoma brucei; D049452:Green Fluorescent Proteins