Qualitative method for determination of aflatoxin B1 in nuts.
The proper characterization of a commercial qualitative method for determining aflatoxin B1 in some nuts is described. A qualitative method that provides binary responses of the yes/no type means that the performance parameters have been properly adapted and defined. Performance characteristics such as the cut-off limit, the detection limit, sensitivity, specificity, false-positive and negative rates, and the unreliability or uncertainty region are defined and then estimated by means of the performance characteristic curves. The commercial test kit showed the cut-off limit at 1.6 ng/g, with a sensitivity rate of 95% and a false-negative rate of zero. A modification can be performed to shift the cut-off to 2.0 ng/g, keeping the same values for the sensitivity and false-negative rate.
Trullols
Esther
E
Universitat Rovira i Virgili, Departament de Química Analítica i Química Orgànica, Plaça Imperial Tàrraco 1, 43005 Tarragona, Spain. ets@quimica.urv.es
Ruisánchez
Itziar
I
Rius
F Xavier
FX
Odena
Marta
M
Feliu
M Teresa
MT
D016604:Aflatoxin B1 / Q000032:analysis*; D009754:Nuts / Q000737:chemistry*; D011933:Reagent Kits, Diagnostic
D011933:Reagent Kits, Diagnostic; D016604:Aflatoxin B1