title: Effect of erythromycin on chronic respiratory infection caused by Pseudomonas aeruginosa with biofilm formation in an experimental murine model.
abstract: Diffuse panbronchiolitis (DPB) is a chronic lower respiratory tract infection commonly associated with persistent late-stage Pseudomonas aeruginosa infection. However, low-dose long-term therapy with certain macrolides is effective in most patients with DPB. The present study was designed to examine the effects of long-term erythromycin (ERY) therapy by using our established murine model of chronic respiratory P. aeruginosa infection. ERY or saline was administered from day 80 after intubation with a P. aeruginosa-precoated tube for the subsequent 10, 20, 40, and 80 days. Bacteriologic and histologic analyses of the murine lungs and electron microscopy of the intubated tube were performed. In the murine model, treatment with ERY for 80 days significantly reduced the number of viable P. aeruginosa organisms in the lungs (P < 0.05). The biofilm formed in situ by P. aeruginosa on the inner wall of the inoculation tube placed into the murine bronchus became significantly thinner after 80 days of ERY treatment. We conclude that the clinical efficacy of macrolides in DPB may be due at least in part to the reduction in P. aeruginosa biofilm formation.
authors.affiliation: Second Department of Internal Medicine, School of Medicine, Nagasaki University, 1-7-1 Sakamoto, Nagasaki 852, Japan.
authors.forename: Towako
authors.initials: T
authors.lastname: Nagata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mukae
authors.forename: Junichi
authors.initials: J
authors.lastname: Kadota
authors.forename: Tomayoshi
authors.initials: T
authors.lastname: Hayashi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fujii
authors.forename: Misuzu
authors.initials: M
authors.lastname: Kuroki
authors.forename: Ryo
authors.initials: R
authors.lastname: Shirai
authors.forename: Katsunori
authors.initials: K
authors.lastname: Yanagihara
authors.forename: Kazunori
authors.initials: K
authors.lastname: Tomono
authors.forename: Takehiko
authors.initials: T
authors.lastname: Koji
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kohno
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D018441:Biofilms / Q000187:drug effects*; D001982:Bronchial Diseases / Q000188:drug therapy / Q000382:microbiology / Q000473:pathology; D002908:Chronic Disease; D004917:Erythromycin / Q000627:therapeutic use*; D008168:Lung / Q000382:microbiology / Q000473:pathology; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011552:Pseudomonas Infections / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D012141:Respiratory Tract Infections / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology
chemical_list: D000900:Anti-Bacterial Agents; D004917:Erythromycin