Quantification of cow milk adulteration in goat milk using high-performance liquid chromatography with electrospray ionization mass spectrometry.
A method was developed for the quantification of cow milk adulteration in goat milk, based on solvent separation of whey proteins followed by high-performance liquid chromatography with electrospray ionization mass spectrometry (HPLC/ESI-MS). The presence of cow milk was determined using beta-lactoglobulin whey protein as the molecular marker. The adulterants were identified using both retention time and molecular mass derived from multiply charged molecular ions. Standard solutions containing cow and goat milk in different volume ratios were prepared and analyzed. Good linearity covering cow milk content from 5% and above was obtained. The proposed method identifies the adulterants using accurate molecular masses for protein identification and detects the addition of cow milk to goat milk at levels as low as 5%.
Chen
Ren-Kun
RK
Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan 300.
Chang
Li-Wen
LW
Chung
Ya-Yun
YY
Lee
Ming-Hsung
MH
Ling
Yong-Chien
YC
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005504:Food Analysis / Q000379:methods* / Q000592:standards; D005506:Food Contamination / Q000032:analysis*; D006041:Goats; D007782:Lactoglobulins / Q000302:isolation & purification; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000302:isolation & purification; D012015:Reference Standards; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods* / Q000592:standards; D000067816:Whey Proteins
D007782:Lactoglobulins; D008894:Milk Proteins; D000067816:Whey Proteins