Cloning, expression and partial characterization of a Haemaphysalis longicornis and a Rhipicephalus appendiculatus glutathione S-transferase.
The ticks Haemaphysalis longicornis and Rhipicephalus appendiculatus are important parasites worldwide. The current method for control of cattle ticks involves the use of chemicals. Nevertheless, parasite resistance is an ever increasing global problem. Glutathione S-transferases (GSTs) play a central role in detoxication of xenobiotic and endogenous compounds. Several authors have noted that an increase in GST activity is associated with resistance to insecticides and acaricides. In the present study, we report the cloning and expression of GST cDNAs from H. longicornis and R. appendiculatus. In addition, we determine the effect of three acaricides (ethion, deltamethrin and diazinon) on the enzymatic activity of rGSTs.
da Silva Vaz Jnr
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Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido, Japan.
Imamura
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Ohashi
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Onuma
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M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D003124:Colorimetry; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D003976:Diazinon / Q000633:toxicity; D004789:Enzyme Activation / Q000187:drug effects; D015870:Gene Expression; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism*; D007305:Insecticide Resistance / Q000235:genetics; D026863:Ixodidae / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009570:Nitriles; D009946:Organothiophosphorus Compounds / Q000633:toxicity; D011722:Pyrethrins / Q000633:toxicity; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D018076:DNA, Complementary; D009570:Nitriles; D009946:Organothiophosphorus Compounds; D011722:Pyrethrins; C017180:decamethrin; C100038:ethion; D005982:Glutathione Transferase; D003976:Diazinon