title: Bone marrow stromal cells infused into the cerebrospinal fluid promote functional recovery of the injured rat spinal cord with reduced cavity formation.
abstract: The effects of bone marrow stromal cells (BMSCs) on the repair of injured spinal cord and on the behavioral improvement were studied in the rat. The spinal cord was injured by contusion using a weight-drop at the level of T8-9, and the BMSCs from the bone marrow of the same strain were infused into the cerebrospinal fluid (CSF) through the 4th ventricle. BMSCs were conveyed through the CSF to the spinal cord, where most BMSCs attached to the spinal surface although a few invaded the lesion. The BBB score was higher, and the cavity volume was smaller in the rats with transplantation than in the control rats. Transplanted cells gradually decreased in number and disappeared from the spinal cord 3 weeks after injection. The medium supplemented by CSF (250 microl in 3 ml medium) harvested from the rats in which BMSCs had been injected 2 days previously promoted the neurosphere cells to adhere to the culture dish and to spread into the periphery. These results suggest that BMSCs can exert effects by producing some trophic factors into the CSF or by contacting with host spinal tissues on the reduction of cavities and on the improvement of behavioral function in the rat. Considering that BMSCs can be used for autologous transplantation, and that the CSF infusion of transplants imposes a minimal burden on patients, the results of the present study are important and promising for the clinical use of BMSCs in spinal cord injury treatment.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Kyoto University Graduate School of Medicine, Shogoin, Sakyo-Ku, Kyoto 606-8507, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Ohta
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Toru
authors.initials: T
authors.lastname: Noda
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ejiri
authors.forename: Mari
authors.initials: M
authors.lastname: Dezawa
authors.forename: Kazuya
authors.initials: K
authors.lastname: Kataoka
authors.forename: Hirotomi
authors.initials: H
authors.lastname: Chou
authors.forename: Namiko
authors.initials: N
authors.lastname: Ishikawa
authors.forename: Naoya
authors.initials: N
authors.lastname: Matsumoto
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Iwashita
authors.forename: Eiji
authors.initials: E
authors.lastname: Mizuta
authors.forename: Sadako
authors.initials: S
authors.lastname: Kuno
authors.forename: Chizuka
authors.initials: C
authors.lastname: Ide
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001522:Behavior, Animal; D001854:Bone Marrow Cells / Q000166:cytology*; D016380:Brain Tissue Transplantation / Q000379:methods; D002448:Cell Adhesion; D002465:Cell Movement; D002470:Cell Survival; D002555:Cerebrospinal Fluid / Q000166:cytology* / Q000502:physiology; D004195:Disease Models, Animal; D006085:Graft Survival; D049452:Green Fluorescent Proteins; D006624:Hippocampus / Q000166:cytology / Q000637:transplantation; D007276:Injections, Intraventricular; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D020127:Recovery of Function / Q000187:drug effects / Q000502:physiology*; D013116:Spinal Cord / Q000166:cytology / Q000473:pathology / Q000637:transplantation; D013119:Spinal Cord Injuries / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D017154:Stromal Cells / Q000166:cytology / Q000637:transplantation*; D016896:Treatment Outcome
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins