Evaluation of cellulose-binding domain fused to a lipase for the lipase immobilization.
A cellulose-binding domain (CBD) fragment of a cellulase gene of Trichoderma hazianum was fused to a lipase gene of Bacillus stearothermophilus L1 to make a gene cluster for CBD-BSL lipase. The specific activity of CBD-BSL lipase for oil hydrolysis increased by 33% after being immobilized on Avicel (microcrystalline cellulose), whereas those of CBD-BSL lipase and BSL lipase decreased by 16% and 54%, respectively, after being immobilized on silica gel. Although the loss of activity of an enzyme immobilized by adsorption has been reported previously, the loss of activity of the CBD-BSL lipase immobilized on Avicel was less than 3% after 12 h due to the irreversible binding of CBD to Avicel.
Hwang
Sangpill
S
Department of Chemical Engineering, Yonsei University, Seoul 120-749, Korea.
Ahn
Jungoh
J
Lee
Sumin
S
Lee
Tai Gyu
TG
Haam
Seungjoo
S
Lee
Kangtaek
K
Ahn
Ik-Sung
IS
Jung
Joon-Ki
JK
D002480:Cellulase / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D002482:Cellulose / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000235:genetics; D001411:Geobacillus stearothermophilus / Q000201:enzymology* / Q000235:genetics; D006868:Hydrolysis; D008049:Lipase / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry; D015202:Protein Engineering / Q000379:methods*; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics
D004800:Enzymes, Immobilized; D000069463:Olive Oil; D010938:Plant Oils; D011993:Recombinant Fusion Proteins; D002482:Cellulose; D008049:Lipase; D002480:Cellulase