[Influence of epidermal growth factor and gonadotrophin on the in vitro maturation of human oocytes].
OBJECTIVE
To study the effect of epidermal growth factor(EGF) and different concentrations of gonadotrophin (Gn) on the in vitro maturation of human oocytes.


METHODS
EGF was added to the vitro culture medium in order to observe the effect of Gn combined with or without EGF on the result of in vitro maturation. The concentrations of hCG and FSH were changed respectively to observe the difference between the results.


RESULTS
Adding EGF to the culture medium improved the maturation rate of oocyte significantly (P < 0.05). There was no difference between the results with different concentrations of hCG and FSH in the culture medium.


CONCLUSIONS
EGF can improve the results of the in vitro maturation of human oocytes by increasing the maturation rate significantly. Increasing the concentration of Gn does not influence the results of in vitro maturation.
Li
Yuan
Y
Reproductive Medical Center, Shandong Provincial Hospital, Shandong University, Jinan, Shandong 250021, China. yuanyuantv@sina.com
Chen
Zijiang
Z
Zhao
Lixin
L
Li
Mei
M
Ma
Shuiying
S
Wang
Li
L
Hu
Jingmei
J
D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D005306:Fertilization / Q000187:drug effects; D006062:Gonadotropins / Q000494:pharmacology*; D006801:Humans; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology
D006062:Gonadotropins; D004815:Epidermal Growth Factor