The two subfamilies of rice glutelin differ in both primary and higher-order structures.
Rice glutelin, which accounts for 70-80% of the total proteins of the seeds, consists of two nutritionally different subfamilies (A and B types). Although the similarity in primary sequences between the two subfamilies is as high as 60%, we established conditions to discriminate the two subfamilies when low amounts of antigen are analyzed by immunoblot methods. The glutelin alpha polypeptides can be resolved into six bands labeled alpha1 to alpha6 by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Gel filtration analysis showed that glutelin exists as a polymerized and a smaller molecular weight form. Immunoblot analysis of SDS-PAGE resolved polypeptides showed that alpha2, alpha3, and alpha4 are an A type and that these A types as well as alpha1, a B type, are polymerized. The polymerization tendency clearly differed between the two subfamilies except for alpha1, which may be derived from GluB-4 as suggested by analysis using Escherichia coli expression systems of glutelin cDNA regions corresponding to alpha polypeptides. GluB-4 and all the A type subunits have an extra Cys residue in the hypervariable regions, corresponding to the C-terminal region of alpha polypeptide. Accordingly, the extra Cys residue is hypothesized to be responsible for the polymerization of glutelin.
Katsube-Tanaka
Tomoyuki
T
Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan. tanakat@muses.tottori-u.ac.jp
Duldulao
Joy Bartolome A
JB
Kimura
Yuka
Y
Iida
Shuichi
S
Yamaguchi
Takeshi
T
Nakano
Junichi
J
Utsumi
Shigeru
S
D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D018076:DNA, Complementary / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005983:Glutens / Q000737:chemistry* / Q000235:genetics; D012275:Oryza / Q000737:chemistry* / Q000235:genetics; D017433:Protein Structure, Secondary; D012639:Seeds / Q000737:chemistry / Q000235:genetics
D018076:DNA, Complementary; D005983:Glutens