Capillary electrophoresis in the analysis and monitoring of biotechnological processes.
Capillary electrophoresis (CE) became a versatile technique for analysis of biological macromolecules. We have applied capillary zone electrophoresis (CZE) and SDS-gel CE for the characterization of recombinant proteins during development of major bioprocessing steps, including fermentation, hybridoma cell cultivation, chromatographic purification, and chemical transformation. Rapid SDS-gel CE was developed for the fast analysis of fermentation broth and hybridoma cell culture. The total analysis time was reduced to 4.5 min. We have developed system for fraction collection, which allows analyzing separated proteins by MALDI-TOF-MS. The main advantages of applied techniques were high resolution and selectivity, fast analysis, and high accuracy.
Klyushnichenko
Vadim
V
Altus Biologics Inc., Cambridge, MA., USA.
D000818:Animals; D002478:Cells, Cultured; D019075:Electrophoresis, Capillary / Q000379:methods; D005782:Gels; D006801:Humans; D006825:Hybridomas / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013501:Surface-Active Agents / Q000737:chemistry*
D005782:Gels; D011994:Recombinant Proteins; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate