title: Human herpesvirus 6 (HHV-6) is transmitted from parent to child in an integrated form and characterization of cases with chromosomally integrated HHV-6 DNA.
abstract: We obtained 7,566 peripheral blood mononuclear cell (PBMC) samples from 2,332 individuals and screened them for human herpesvirus infection. We identified five individuals who persistently harbored high copy numbers of human herpesvirus 6 (HHV-6) DNA in their PBMCs. HHV-6 DNA was also detected in other somatic tissues of these individuals. Five additional cases were identified among their family members. For two of these families, chromosomally integrated HHV-6 DNA (CIHHV-6) was detected in the PBMCs by fluorescence in situ hybridization. The prevalence of CIHHV-6 among all the subjects was 0.21%. The HHV-6 DNA was variant B in four families and variant A in one family. Antibodies to immediate early antigen and glycoprotein B were detected in 57 and 14% of individuals with CIHHV-6 and in 0 and 60% of healthy volunteers without CIHHV-6, respectively. HHV-6 could not be isolated from PBMCs with CIHHV-6. These cases shared no clinical features, and included three healthy individuals. Our data suggest that CIHHV-6 is rare but detectable in the general population and that hereditary transmission is one of the routes of HHV-6 transmission.
authors.affiliation: Department of Developmental Medicine (Pediatrics), Osaka University Graduate School of Medicine, Osaka, Japan. ktaya@nih.go.jp
authors.forename: Keiko
authors.initials: K
authors.lastname: Tanaka-Taya
authors.forename: Junji
authors.initials: J
authors.lastname: Sashihara
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kurahashi
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Amo
authors.forename: Hiromi
authors.initials: H
authors.lastname: Miyagawa
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kondo
authors.forename: Shintaro
authors.initials: S
authors.lastname: Okada
authors.forename: Koichi
authors.initials: K
authors.lastname: Yamanishi
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D002877:Chromosomes, Human / Q000821:virology; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006197:Hair / Q000821:virology; D015654:Herpesvirus 6, Human / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D017874:Immediate-Early Proteins / Q000276:immunology; D017404:In Situ Hybridization, Fluorescence; D018445:Infectious Disease Transmission, Vertical; D007963:Leukocytes, Mononuclear / Q000821:virology*; D008297:Male; D009061:Mouth Mucosa / Q000821:virology; D010375:Pedigree; D010614:Pharynx / Q000821:virology; D010750:Phosphoproteins / Q000276:immunology; D016133:Polymerase Chain Reaction; D019349:Roseolovirus Infections / Q000635:transmission* / Q000821:virology*; D014759:Viral Envelope Proteins / Q000276:immunology; D016662:Virus Integration
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D017874:Immediate-Early Proteins; D010750:Phosphoproteins; D014759:Viral Envelope Proteins; C458509:immediate-early 1 protein, human herpesvirus 6; C077311:glycoprotein B, human herpesvirus-6