title: On-chip biosensing of estrogen receptor-alpha at single molecular level.
abstract: A novel method for detecting interaction between DNA and DNA-binding protein at single molecular level has been proposed. In this study, estrogen receptor-alpha (ER-alpha) was used for biosensing as the proof-example. A 518 bp-long (ca. 176 nm) DNA probe labeled with streptavidin at its 5'-terminus was prepared by inserting a consensus oligonucleotide sequence that binds to ER-alpha. A solution containing ER-alpha was dropped onto the Ni-treated mica substrate on which the DNA prove was previously immobilized, and it was observed by AFM. Specific binding of ER-alpha could be observed by measuring the distance between the site where binding occur, to the streptavidin label.
authors.affiliation: Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Dedy H B
authors.initials: DH
authors.lastname: Wicaksono
authors.forename: Takashi
authors.initials: T
authors.lastname: Ebihara
authors.forename: Hisakage
authors.initials: H
authors.lastname: Funabashi
authors.forename: Masayasu
authors.initials: M
authors.lastname: Mie
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Yanagida
authors.forename: Masuo
authors.initials: M
authors.lastname: Aizawa
authors.forename: Eiry
authors.initials: E
authors.lastname: Kobatake
mesh_terms: D001665:Binding Sites; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000648:ultrastructure*; D015342:DNA Probes / Q000032:analysis / Q000737:chemistry / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000032:analysis / Q000737:chemistry / Q000648:ultrastructure; D047628:Estrogen Receptor alpha / Q000032:analysis* / Q000737:chemistry / Q000648:ultrastructure*; D018625:Microscopy, Atomic Force / Q000379:methods*; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D015342:DNA Probes; D004268:DNA-Binding Proteins; D047628:Estrogen Receptor alpha; D004247:DNA