Identification of CD4+ T cell epitopes of Taenia solium paramyosin.
T cell mediated response is involved in a protective immune response against experimental cysticercosis conferred by immunization with Taenia solium paramyosin (TPmy) to BALB/c mice. In this study, we analysed the TPmy amino acid sequence for predicted CD4+ T cells epitopes. Five different regions of this protein showed that the residues anchor to bind the I-Ad molecule, synthetic peptides containing these epitopes were evaluated for their ability to induce lymphoproliferative responses of spleen cells from TPmy immunized mice. Among them, Tp176 (amino acids 176-192 sequence DDLQRQMADANSAKSRL) was the immunodominant T cell epitope of TPmy. Delineation of this epitope should facilitate analysis of the role of CD4+ T cell response in experimental cysticercosis.
López-Moreno
H S
HS
Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del IPN, D.F., México, DF.
Correa
D
D
Laclette
J P
JP
Ortiz-Navarrete
V F
VF
D000595:Amino Acid Sequence; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002455:Cell Division / Q000276:immunology; D003551:Cysticercosis / Q000276:immunology*; D018604:Epitope Mapping; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry* / Q000276:immunology; D005260:Female; D000949:Histocompatibility Antigens Class II; D016056:Immunodominant Epitopes / Q000737:chemistry / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D041201:Taenia solium / Q000276:immunology*; D014335:Tropomyosin / Q000276:immunology*
D018984:Epitopes, T-Lymphocyte; D000949:Histocompatibility Antigens Class II; C111389:I-Ad antigen; D016056:Immunodominant Epitopes; D010446:Peptide Fragments; D014335:Tropomyosin