title: Different cytokine levels in thrombolysis patients as predictors for clinical outcome.
abstract: Thrombolytic therapy not always improves clinical outcome in ischemic stroke patients. This could cause lymphomonocyte accumulation in the infarcted brain area. These produce an excessive amount of proinflammatory cytokines, such as IL-1 beta, IL-6 and TNF-alfa. The aim of our study was to determine ILs levels in fibrinolytic therapy treated patients, compared with healthy controls and to evaluate if the varying levels can predictors of neurological outcome. Eighteen patients underwent thrombolytic treatment with t-PA within 3 h. Plasma levels of IL-1 beta, IL-6, TNF-alfa and IL-10 were determined by ELISA method before and within 24 h after t-PA infusion and compared with controls. Significantly higher levels of IL-1 beta and Il-6 emerged in stroke patients before treatment compared with the control group (P < 0.05 and 0.04, respectively). Slightly higher plasma levels of TNF-alfa and lower plasma levels of IL-10 were also found at base line in stroke patients. After thrombolytic treatment no significant variations were observed in the levels of TNF-alfa and IL-6, whereas a trend toward lower values for IL-1 beta and higher levels for IL-10 was observed. Positive correlations among the values of IL-6, TNF-alfa and National Institute of Health Stroke Scale (NIHSS) at discharges were observed. A similar correlation with modified Rankin scale score at 3 month was found. Pre-treatment cytokine status seems to influence pre-and long-term clinical outcome. Therefore an investigation into the possible predictor of cytokines seem worthy.
authors.affiliation: Stroke Unit, Department of Neuroscience, University of Perugia, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Mazzotta
authors.forename: P
authors.initials: P
authors.lastname: Sarchielli
authors.forename: V
authors.initials: V
authors.lastname: Caso
authors.forename: M
authors.initials: M
authors.lastname: Paciaroni
authors.forename: A
authors.initials: A
authors.lastname: Floridi
authors.forename: A
authors.initials: A
authors.lastname: Floridi
authors.forename: V
authors.initials: V
authors.lastname: Gallai
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D016207:Cytokines / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005343:Fibrinolytic Agents / Q000627:therapeutic use*; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011379:Prognosis; D012307:Risk Factors; D018709:Statistics, Nonparametric; D020521:Stroke / Q000175:diagnosis / Q000188:drug therapy* / Q000378:metabolism; D015912:Thrombolytic Therapy / Q000379:methods; D013997:Time Factors; D010959:Tissue Plasminogen Activator / Q000627:therapeutic use*; D016896:Treatment Outcome
chemical_list: D016207:Cytokines; D005343:Fibrinolytic Agents; D010959:Tissue Plasminogen Activator