Inactivation of pathogenic bacteria by cucumber volatiles (E,Z)-2,6-nonadienal and (E)-2-nonenal.
The effects of (E,Z)-2,6-nonadienal (NDE) and (E)-2-nonenal (NE) on Bacillus cereus, Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella Typhimurium were investigated. A suspension of each organism of 6 to 9 log CFU/ml was incubated for 1 h at 37 degrees C in brain heart infusion solution that contained 0 to 500 or 1,000 ppm of NDE or NE. Depending on concentration, exposure to either NDE or NE caused a reduction in CFU of each organism. Treatment with 250 and 500 ppm NDE completely eliminated viable B. cereus and Salmonella Typhimurium cells, respectively. L. monocytogenes was the most resistant to NDE, showing only about a 2-log reduction from exposure to 500 ppm for 1 h. Conversely, this concentration of NDE caused a 5.8-log reduction in E. coli O157:H7 cells. NE was also effective in inactivating organisms listed above. A higher concentration of NE, 1,000 ppm, was required to kill E. coli O157:H7, L. monocytogenes, or Salmonella Typhimurium compared with NDE. In conclusion, both NDE and NE demonstrated an apparent bactericidal activity against these pathogens.
Cho
M J
MJ
Department of Food Science and Institute of Food Science and Engineering, University of Arkansas, 2650 North Young Avenue, Fayetteville, Arkansas 72704, USA.
Buescher
R W
RW
Johnson
M
M
Janes
M
M
D000447:Aldehydes / Q000494:pharmacology*; D001409:Bacillus cereus / Q000187:drug effects* / Q000254:growth & development; D015169:Colony Count, Microbial; D018553:Cucumis sativus / Q000382:microbiology*; D004305:Dose-Response Relationship, Drug; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000254:growth & development; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000254:growth & development; D013696:Temperature; D013997:Time Factors; D014835:Volatilization
D000447:Aldehydes