Hepatoprotective effect of Sabina przewalskii against menadione-induced toxicity.
Sabina przewalskii is a Tibetan medicinal plant. Pretreatment with an ethyl acetate-soluble fraction of the plant suppressed the plasma alanine aminotransferase activity in menadione-intoxicated mice. In vitro studies showed that the Sabina extract could inhibit NADH-induced superoxide production in isolated hepatocytes as indicated by a decrease of lucigenin-amplified chemiluminescence. In addition, the extract inhibited tert-butyl hydroperoxide-induced lipid peroxidation in isolated hepatocytes dose-dependently. These results suggested that the protective action of the Sabina extract against menadione-induced hepatotoxicity is associated with its antioxidant activities including the scavenging of superoxide anion radicals and inhibition of lipid peroxidation.
Ip
Siu-Po
SP
School of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong.
Woo
Ka-Yan
KY
Lau
Oi-Wah
OW
Che
Chun-Tao
CT
D000410:Alanine Transaminase / Q000097:blood / Q000187:drug effects; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D005260:Female; D022781:Hepatocytes / Q000187:drug effects; D018653:Juniperus; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009243:NAD; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D020011:Protective Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D024483:Vitamin K 3
D010936:Plant Extracts; D020011:Protective Agents; D009243:NAD; D024483:Vitamin K 3; D000410:Alanine Transaminase