title: Importance of hydrophobic cluster formation through long-range contacts in the folding transition state of two-state proteins.
abstract: Understanding the folding pathways of proteins is a challenging task. The Phi value approach provides a detailed understanding of transition-state structures of folded proteins. In this work, we have computed the hydrophobicity associated with each residue in the folded state of 16 two-state proteins and compared the Phi values of each mutant residue. We found that most of the residues with high Phi value coincide with local maximum in surrounding hydrophobicity, or have nearby residues that show such maximum in hydrophobicity, indicating the importance of hydrophobic interactions in the transition state. We have tested our approach to different structural classes of proteins, such as alpha-helical, SH3 domains of all-beta proteins, beta-sandwich, and alpha/beta proteins, and we observed a good agreement with experimental results. Further, we have proposed a hydrophobic contact network pattern to relate the Phi values with long-range contacts, which will be helpful to understand the transition-state structures of folded proteins. The present approach could be used to identify potential hydrophobic clusters that may form through long-range contacts during the transition state.
authors.affiliation: Department of Physics, Bharathidasan University, Tamilnadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Selvaraj
authors.forename: M Michael
authors.initials: MM
authors.lastname: Gromiha
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry; D002268:Carboxypeptidases / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D010455:Peptides / Q000737:chemistry; D010940:Plant Proteins; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry; D000097024:Acylphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D000596:Amino Acids; D002268:Carboxypeptidases; D010455:Peptides; D010940:Plant Proteins; D011506:Proteins; D000097024:Acylphosphatase; C093336:chymotrypsin inhibitor 2