title: In vitro biocompatibility assessment of poly(epsilon-caprolactone) films using L929 mouse fibroblasts.
abstract: Biodegradable and biocompatible materials are the basis for tissue engineering. As an initial step for developing vascular grafts, the in vitro biocompatibility of poly(epsilon-caprolactone) (PCL), recently suggested for several clinical applications, was evaluated in this study using L929 mouse fibroblasts. Different cellular aspects were analyzed in order to know the cell viability during cell culture on PCL films: adhesion, proliferation, morphology, LDH release and mitochondrial function. Since topography and other surface characteristics of materials play an essential part in cell adhesion, PCL membranes with either smooth or rough surface were prepared, characterized and used to carry out cell cultures. During short culture times, PCL produced a significant stimulation of mitochondrial activity evaluated by reduction of the MTT reagent. The results provide evidences of good adhesion, growth, viability, morphology and mitochondrial activity of cells on PCL films. Therefore, it can be concluded that PCL is a suitable and biocompatible material as a scaffold for vascular graft development.
authors.affiliation: Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense, Madrid-28040, Spain.
authors.forename: M C
authors.initials: MC
authors.lastname: Serrano
authors.forename: R
authors.initials: R
authors.lastname: Pagani
authors.forename: M
authors.initials: M
authors.lastname: Vallet-Regí
authors.forename: J
authors.initials: J
authors.lastname: Peña
authors.forename: A
authors.initials: A
authors.lastname: Rámila
authors.forename: I
authors.initials: I
authors.lastname: Izquierdo
authors.forename: M T
authors.initials: MT
authors.lastname: Portolés
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002452:Cell Count; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D042963:Electron Transport Complex II / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism* / Q000648:ultrastructure; D005898:Glass / Q000737:chemistry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008422:Materials Testing; D008567:Membranes, Artificial; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D013499:Surface Properties; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
chemical_list: D008567:Membranes, Artificial; D011091:Polyesters; D011137:Polystyrenes; D013778:Tetrazolium Salts; D013844:Thiazoles; C016240:polycaprolactone; D007770:L-Lactate Dehydrogenase; D042963:Electron Transport Complex II; C022616:thiazolyl blue