A validated HPLC method with ultraviolet detection for the determination of buformin in plasma.
A sensitive and rapid high-performance liquid chromatographic assay is developed and validated for the determination of buformin in plasma. After addition of metformin as the internal standard, the analytes were deproteinated with acetonitrile, washed with dichloromethane, and the resulting supernatant injected. Chromatography was performed at ambient temperature by pumping a mobile phase of 0.03 m diammonium hydrogen phosphate buffer (pH 7, 250 mL) in methanol (750 mL) at a fl ow rate of 1 mL/min through a silica column. Buformin and metformin were detected at 236 nm, and eluted 9.8 and 15.4 min, respectively. No endogenous substances were found to interfere. Calibration curves were linear over the concentration range of 20-2000 ng/mL. The limit of quantitation was 20 ng/mL. The intra- and inter-day relative standard deviation (RSD) was 8.3%, or less, and the accuracy was within 10.1% of the relative error (RE). The method is suitable in pharmacokinetic investigation of buformin.
Chou
Chen-Hsi
CH
Institute of Clinical Pharmacy, Medical College, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan.
Cheng
Ching-Ling
CL
Huang
Chien-Chen
CC
D000818:Animals; D014150:Antipsychotic Agents / Q000097:blood; D002026:Buformin / Q000097:blood* / Q000737:chemistry; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007004:Hypoglycemic Agents / Q000097:blood; D008297:Male; D008687:Metformin / Q000097:blood* / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D014150:Antipsychotic Agents; D007004:Hypoglycemic Agents; D008687:Metformin; D002026:Buformin