[Preparation of rabbit anti-Syk antibody and its application in breast cancer diagnosis].
OBJECTIVE
To prepare rabbit anti-Syk antibody and analysis its application in breast cancer diagnosis.


METHODS
The rabbits were immunized with recombinant mouse Syk protein to prepare antibody against Syk. The purity and specificity of the antibody were analyzed by Western blot. The expression of Syk protein was detected by immunohistochemical staining.


RESULTS
The titer of serum anti-Syk antibody was 1:6 400 (A450 = 1. 03) 6 weeks after the immunization. The SDS-PAGE and Western blot analysis showed that there was a obvious protein band with Mr 72,000, confirming the specificity of obtained anti-Syk antibody. It was displayed by immunohistochemical staining with anti-Sykantibody that in normal breast tissue Syk was high expression; while in infilfrative dustal carcinoma of breast tissue the expression of syk was negative.


CONCLUSIONS
The rabbit anti-Syk antibody with high titer and specificity has been prepared. The Syk is high expression in normal breast tissue, but is deficient in breast cancer tissue. The result may be useful for clinical diagnosis of breast cancer.
Shan
Bao-en
BE
Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China. baoenshan@yahoo.com.cn
Dong
Qing
Q
Wang
Xiao-ling
XL
Chen
Xing
X
Dong
Jin-zhuo
JZ
Ma
Hong
H
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification*; D000918:Antibody Specificity; D001943:Breast Neoplasms / Q000737:chemistry / Q000175:diagnosis*; D004792:Enzyme Precursors / Q000032:analysis / Q000276:immunology*; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D011505:Protein-Tyrosine Kinases / Q000032:analysis / Q000276:immunology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D000072377:Syk Kinase
D000906:Antibodies; D004792:Enzyme Precursors; D047908:Intracellular Signaling Peptides and Proteins; D011994:Recombinant Proteins; D011505:Protein-Tyrosine Kinases; D000072377:Syk Kinase; C000606897:Syk protein, mouse