Biocompatibility of poly(epsilon-caprolactone)/poly(ethylene glycol) diblock copolymers with nanophase separation.
In this study, we prepared diblock copolymers of poly(epsilon-caprolactone) (PCL) and poly(ethylene glycol) (PEG) by aluminum alkoxide catalysts. The biological responses to the spin cast surface of different PCL/PEG diblock copolymers were investigated in vitro. Our results showed that surface hydrophilicity improved with the increased PEG segments in diblock copolymers and that bacteria adhesion was inhibited by increased PEG contents. PCL-PEG 23:77 showed nanotopography on the surface. The number of adhered endothelial cells, platelets and monocytes on diblock copolymer surfaces was inhibited in PCL-PEG 77:23 and enhanced in PCL-PEG 23:77. Nevertheless, the platelet and monocyte activation on PCL-PEG 23:77 was reduced. PCL-PEG 23:77 had better cellular response as well as lower degree of platelet and monocyte activation. The current study was the first one to demonstrate that surface nanotopography could influence not only cell adhesion and growth but also platelet and monocyte activation.
Hsu
Shan-Hui
SH
Department of Chemical Engineering, National Chung Hsing University, Taichung, Taiwan. shhsu@nchu.edu.tw
Tang
Cheng-Ming
CM
Lin
Chu-Chieh
CC
D001412:Bacillus subtilis / Q000502:physiology; D001422:Bacterial Adhesion / Q000502:physiology; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D001792:Blood Platelets / Q000166:cytology / Q000502:physiology / Q000648:ultrastructure; D002448:Cell Adhesion / Q000502:physiology; D049109:Cell Proliferation; D003599:Cytoskeleton / Q000737:chemistry; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008262:Macrophage Activation / Q000502:physiology; D009682:Magnetic Resonance Spectroscopy; D008422:Materials Testing; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D009000:Monocytes / Q000502:physiology; D015539:Platelet Activation / Q000502:physiology; D010973:Platelet Adhesiveness; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties
D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011091:Polyesters; C405621:poly(epsilon-caprolactone-co-ethylene oxide); C016240:polycaprolactone; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols