The osmotic characteristics of human fetal liver-derived hematopoietic stem cell candidates.
Hematopoietic stem cells derived from fetal liver have promising therapeutic potential for allotransplantation but require a specific protocol to minimize the damage produced by cryopreservation procedures. In this study, a fundamental approach was applied for designing a cell preservation protocol. To this end, the biophysical characteristics that describe the osmotic reaction of CD34(+)CD38(-) human fetal liver stem cell candidates were studied using fluorescent microscopy. The osmotically inactive volume of the stem cell candidates was determined as 48% of the isotonic volume. The permeability coefficients for water and Me(2)SO were determined at T = +22 degree C: L(p) = 0.27 +/- 0.03 microm x min(-1)atm(-1), P(Me(2)SO)) = 2.09 +/- 0.85 x 10 (-4) cm x min(-1), sigma (Me(2)SO)) = 0.63 +/- 0.03 and at T = +12 degree C: L(p) = 0.15 +/-0.02 microm x min(-1)atm(-1), P(Me(2)SO)) = 6.44 +/-1.42 x 10 (-5) cm x min(-1), sigma (Me(2)SO)) = 0.46 +/- 0.05. The results obtained suggest that post-hypertonic and hypotonic stress are the possible reasons for damage to a CD34(+)CD38(-) cell during the cryopreservation procedure.
Tarasov
A I
AI
Institute for Problems of Cryobiology and Cryomedicine of the NASU, Kharkov, Ukraine.
Petrenko
A Yu
AY
Jones
D R E
DR
D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34 / Q000032:analysis; D001793:Blood Preservation; D002463:Cell Membrane Permeability / Q000187:drug effects; D048429:Cell Size; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D006023:Glycoproteins / Q000032:analysis; D006412:Hematopoietic Stem Cells; D006801:Humans; D016130:Immunophenotyping; D017493:Leukocyte Common Antigens / Q000032:analysis; D008099:Liver / Q000166:cytology / Q000196:embryology; D009995:Osmosis; D010455:Peptides / Q000032:analysis; D013696:Temperature; D014867:Water / Q000378:metabolism
D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D003451:Cryoprotective Agents; D006023:Glycoproteins; D010455:Peptides; D014867:Water; D017493:Leukocyte Common Antigens; D004121:Dimethyl Sulfoxide