Development of an isotope-coded activity-based probe for the quantitative profiling of cysteine proteases.
Quantification studies of complex protein mixtures have been restricted mainly to whole cell extracts. Here we describe the synthesis of two sets of isotope-coded activity-based probes that allow quantitative functional proteomics experiments on the cathepsins.
van Swieten
Paul F
PF
Gorlaeus Laboratories, Leiden University, Einsteinweg 55, 2300 RA Leiden, The Netherlands.
Maehr
Rene
R
van den Nieuwendijk
Adrianus M C H
AM
Kessler
Benedikt M
BM
Reich
Michael
M
Wong
Chung-Sing
CS
Kalbacher
Hubert
H
Leeuwenburgh
Michiel A
MA
Driessen
Christoph
C
van der Marel
Gijsbert A
GA
Ploegh
Hidde L
HL
Overkleeft
Herman S
HS
D003546:Cysteine Endopeptidases / Q000032:analysis / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods*; D007554:Isotopes / Q000138:chemical synthesis / Q000378:metabolism; D040901:Proteomics / Q000379:methods
D007554:Isotopes; D003546:Cysteine Endopeptidases