Overexpression of the neuropilin 1 (NRP1) gene correlated with poor prognosis in human glioma.
We studied whether the expression of the Neuropilin (NRP) gene was correlated with clinicopathological features in glioma. We examined the gene expression of vascular endothelial growth factor (VEGF)-A, Flt-1, KDR, NRP1 and NRP2 in 37 gliomas by real time reverse transcriptase PCR (real time RT-PCR) as well as immunohistochemical analysis. The vascular counts of each tumor were evaluated by anti-CD34 antibody. NRP1 mRNA overexpression was significantly higher in neoplastic tissue compared to normal brain tissue samples. The higher grade of glioma overexpressed the NRP1 gene significantly (p=0.0015). The glioma patients with NRP1 overexpression showed a poorer prognosis (p=0.0202) than those without such overexpression. NRP1 was observed in the glioma cells by immunohistochemical analyses. VEGF-A and VEGFR overexpression did not show any correlation with the clinicopathological features, including NRP expression. These results suggest that NRP1 overexpression, rather than VEGF-A or VEGFR, contributes to tumor progression and has clinical significance for glioma.
Osada
Hideo
H
Department of Pathology, Tokai University, School of Medicine, Bohseidai, Isehara, Kanagawa 259-1143, Japan.
Tokunaga
Tetsuji
T
Nishi
Masatake
M
Hatanaka
Hiroyuki
H
Abe
Yoshiyuki
Y
Tsugu
Atsushi
A
Kijima
Hiroshi
H
Yamazaki
Hitoshi
H
Ueyama
Yoshito
Y
Nakamura
Masato
M
D000328:Adult; D001932:Brain Neoplasms / Q000098:blood supply / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015870:Gene Expression; D005910:Glioma / Q000098:blood supply / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism; D039942:Neuropilin-1 / Q000096:biosynthesis* / Q000235:genetics; D039943:Neuropilin-2 / Q000096:biosynthesis / Q000235:genetics; D011379:Prognosis; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000096:biosynthesis / Q000235:genetics; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000096:biosynthesis / Q000235:genetics
D039943:Neuropilin-2; D039942:Neuropilin-1; D040281:Vascular Endothelial Growth Factor Receptor-1; D040301:Vascular Endothelial Growth Factor Receptor-2