Technique review: how to use RNA interference.
RNA interference (RNAi) has been rapidly adopted as a general method for inhibiting gene expression in most laboratory organisms. This paper discusses how libraries of RNAi reagents are being used to perform genome-wide reverse genetic screens in both model organisms and mammalian cells. Guidelines for designing effective small interfering RNAs and appropriate controls for mammalian RNAi experiments will also be discussed.
Lehner
Ben
B
Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK. bll@sanger.ac.uk
Fraser
Andrew G
AG
Sanderson
Christopher M
CM
D000818:Animals; D002478:Cells, Cultured; D015723:Gene Library; D005821:Genetic Techniques; D006801:Humans; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D011995:Recombination, Genetic
D012333:RNA, Messenger; D034741:RNA, Small Interfering