Regulatory regions of smeDEF in Stenotrophomonas maltophilia strains expressing different amounts of the multidrug efflux pump SmeDEF.
The smeT-smeDEF region and the smeT gene, which encodes the smeDEF repressor, are highly polymorphic. Few changes in smeT might be associated with smeDEF overexpression. The results obtained with cellular extracts suggest that mutant SmeT proteins cannot bind to the operator and that other transcription factors besides SmeT are involved in the regulation of smeDEF expression.
Sanchez
Patricia
P
Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, CSIC, Campus Universidad Autónoma de Madrid, Cantoblanco, 28049-Madrid, Spain.
Alonso
Ana
A
Martinez
Jose L
JL
D018435:ATP Binding Cassette Transporter, Subfamily B / Q000096:biosynthesis / Q000235:genetics*; D019943:Amino Acid Substitution; D005798:Genes, Bacterial / Q000235:genetics*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D020615:Stenotrophomonas maltophilia / Q000235:genetics*; D014158:Transcription, Genetic
D018435:ATP Binding Cassette Transporter, Subfamily B