Identification and molecular characterization of myosin gene family in Oryza sativa genome.
Myosins play an important role in various developmental processes in plants. We have identified 14 myosin genes in rice (Oryza sativa cv. Nipponbare) genome using sequence information available in public databases. Phylogenetic analysis of these sequences with other plant and non-plant myosins revealed that two of the predicted sequences belonged to class VIII and the others to class XI. All of these genes were distributed on seven chromosomes in the rice genome. Domain searches on these sequences indicated that a typical rice myosin consisted of Myosin_N, head domain, neck (IQ motifs), tail, and dilute (DIL) domain. Based on the sequence information obtained from predicted myosins, we isolated and sequenced two full-length cDNAs, OsMyoVIIIA and OsMyoXIE, representing each of the two classes of myosins. These two cDNAs isolated from different organs existed in isoforms due to differential splicing and showed minor differences from the predicted myosin in exon organization. Out of 14 myosin genes 11 were expressed in three major organs: leaves, panicles, and roots, among which three myosins exhibited different expression levels. On the other hand, three of the total myosin sequences showed organ-specific expression. The existence of different myosin genes and their isoforms in different organs or tissues indicates the diversity of myosin functions in rice.
Jiang
ShuYe
S
Rice Functional Genomics Group, Temasek Life Sciences Laboratory, 1 Research Link, the National University of Singapore, Singapore 117604.
Ramachandran
Srinivasan
S
D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D030541:Databases, Genetic; D019143:Evolution, Molecular; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D018745:Genome, Plant; D008969:Molecular Sequence Data; D005810:Multigene Family; D009218:Myosins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D010940:Plant Proteins; D020033:Protein Isoforms; D009218:Myosins