title: [Effect of glia cell line-derived neurotrophic factor on cultured spinal motor neurons from embryonic rat].
abstract: OBJECTIVE
  To observe the effects of various concentrations of gli-a cell line-derived neurotrophic factor ( GDNF) on the growth of cultured spinal motor neurons from embryonic rat.
  
  
  METHODS
  Neurons were isolated from embryonic rat spi-nal ventral tissue and then were primarily cultured in vitro. Morphological feature of neurons was examined by immuno-cytochemical staining with mAb SMI-32. The effect of GDNFon growth of spinal motor neurons was detected by MTT colorimetry.
  
  
  RESULTS
  GDNF could significantly enhance the survival and neurite outgrowth of rat spinal motor neurons cultured in vitro in dose-dependent tendency.
  
  
  CONCLUSIONS
  Varying concentrations of GDNF can enhance the growth of embryonic rat spinal motor neurons to various extent.
authors.affiliation: Department of Neurology,Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
authors.forename: Xue-qin
authors.initials: XQ
authors.lastname: Song
authors.forename: Shu-yu
authors.initials: SY
authors.lastname: Wu
authors.forename: Chun-yan
authors.initials: CY
authors.lastname: Li
authors.forename: Li-qin
authors.initials: LQ
authors.lastname: Wang
authors.forename: Xiao-juan
authors.initials: XJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D051100:Glial Cell Line-Derived Neurotrophic Factor; D007150:Immunohistochemistry; D008297:Male; D009046:Motor Neurons / Q000187:drug effects* / Q000502:physiology; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000187:drug effects* / Q000196:embryology / Q000502:physiology
chemical_list: C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors