title: State-of-the-art serological techniques for detection of antibodies against tick-borne encephalitis virus.
abstract: Indirect immunofluorescence, ELISA and immunoblot test systems for the detection of antibodies against tick-borne encephalitis (TBE) virus were developed and evaluated. Sera from 112 patients with clinically characterized TBE virus infections, 27 patients with antibodies against dengue or yellow fever virus and 100 healthy blood donors were investigated for anti-TBE virus antibodies. The assays yielded sensitivities of 91-95% and specificities of 91-94%. The test systems are valuable new tools for the diagnosis and monitoring of TBE virus infections.
authors.affiliation: EUROIMMUN AG, Lübeck, Germany. k.sonnenberg@euroimmun.de
authors.forename: Karen
authors.initials: K
authors.lastname: Sonnenberg
authors.forename: Matthias
authors.initials: M
authors.lastname: Niedrig
authors.forename: Katja
authors.initials: K
authors.lastname: Steinhagen
authors.forename: Edda
authors.initials: E
authors.lastname: Rohwäder
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Meyer
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schlumberger
authors.forename: Ewald
authors.initials: E
authors.lastname: Müller-Kunert
authors.forename: Winfried
authors.initials: W
authors.lastname: Stöcker
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis* / Q000097:blood; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology*; D004675:Encephalitis, Tick-Borne / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral