Pemphigus vulgaris autoantibodies induce apoptosis in HaCaT keratinocytes.
Pemphigus vulgaris (PV) is an autoimmune disease characterized by binding of IgG autoantibodies to epidermal keratinocyte desmosomes. IgG autoantibodies obtained from a patient with mucocutaneous PV reacted with plakoglobin (Plkg) in addition to desmoglein-3 (Dsg3) and Dsg1. Immunofluorescence analysis confirmed that IgG autoantibodies, unlike antibodies from a healthy volunteer, caused disruption of cell-cell contacts in HaCaT keratinocytes. Moreover, apoptosis was enhanced in cells treated with autoantibodies compared to those treated with normal antibodies. The apoptotic process induced by IgG autoantibodies was characterized by caspase-3 activation, Bcl-2 depletion and Bax expression. The present report demonstrates that PV IgG autoantibodies promote apoptosis in HaCaT keratinocytes.
Pelacho
B
B
Department of Biochemistry, University of Navarra, Apartado 177, 31080 Pamplona, Spain.
Natal
C
C
España
A
A
Sánchez-Carpintero
I
I
Iraburu
M J
MJ
López-Zabalza
M J
MJ
D017209:Apoptosis / Q000276:immunology*; D001323:Autoantibodies / Q000276:immunology / Q000494:pharmacology*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002461:Cell Line, Transformed; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000378:metabolism; D053938:DNA Fragmentation; D004306:Dose-Response Relationship, Immunologic; D007074:Immunoglobulin G / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism / Q000648:ultrastructure; D008856:Microscopy, Fluorescence; D010392:Pemphigus / Q000276:immunology*; D011233:Precipitin Tests; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D013997:Time Factors; D051028:bcl-2-Associated X Protein
D001323:Autoantibodies; D003598:Cytoskeletal Proteins; D007074:Immunoglobulin G; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D053148:Caspase 3; D020169:Caspases