title: Tegumental surface changes in adult Fasciola hepatica following treatment in vitro and in vivo with an experimental fasciolicide.
abstract: Our objective was to determine by scanning electron microscopy the structural changes in the tegument of adult Fasciola hepatica after treatment with 5-chloro-2-methylthio-6-(1-naphtyloxy)-1 H-benzimidazole, called compound alpha, and its active metabolite sulphoxide, under in vitro and in vivo conditions. For the in vitro studies, flukes from sheep were exposed to 40 mg/l of compound alpha-sulphoxide over different incubation times. Flukes for the in vivo studies were raised in sheep treated orally with compound alpha and killed at different times post-treatment. Non-treated controls were included for each time of incubation. The results showed lesions after 6 h of treatment, such as swelling and furrows. At 12 h, the spines appeared to be surrounded by the tegument. At 24 h the tegument in some areas showed an exposed basal lamina. These changes became more severe as the incubation periods of the treated flukes increased. Compound alpha exerts a significant effect on the tegument of F. hepatica.
authors.affiliation: Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Mexico City, Mexico. normariv@hotmail.com
authors.forename: Norma
authors.initials: N
authors.lastname: Rivera
authors.forename: Froylán
authors.initials: F
authors.lastname: Ibarra
authors.forename: Armando
authors.initials: A
authors.lastname: Zepeda
authors.forename: Teresa
authors.initials: T
authors.lastname: Fortoul
authors.forename: Alicia
authors.initials: A
authors.lastname: Hernández
authors.forename: Rafael
authors.initials: R
authors.lastname: Castillo
authors.forename: Germinal
authors.initials: G
authors.lastname: Cantó
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D004817:Epidermis / Q000187:drug effects* / Q000648:ultrastructure; D005210:Fasciola hepatica / Q000187:drug effects* / Q000648:ultrastructure; D005211:Fascioliasis / Q000188:drug therapy / Q000662:veterinary*; D007093:Imidazoles / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D008297:Male; D008855:Microscopy, Electron, Scanning / Q000662:veterinary; D009281:Naphthalenes / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D012757:Sheep Diseases / Q000469:parasitology; D013454:Sulfoxides / Q000494:pharmacology*
chemical_list: C468032:5-chloro-2-methylthio-6-(1-naphthyloxy)-1H-benzimidazole; D000871:Anthelmintics; D007093:Imidazoles; D009281:Naphthalenes; D013454:Sulfoxides