Proposal to accommodate Burkholderia cepacia genomovar VI as Burkholderia dolosa sp. nov.
Phenotypic and genotypic studies revealed new tools for differentiating Burkholderia cepacia genomovar VI from Burkholderia multivorans and other B. cepacia-complex species. Hence, the name Burkholderia dolosa sp. nov. is proposed, with LMG 18943(T) (=CCUG 47727(T)) as the type strain. B. dolosa can be differentiated from other B. cepacia-complex bacteria by its inability to assimilate tryptamine, azelaic acid and salicin and by its failure to grow on the B. cepacia-selective medium PCAT. Both 16S rDNA and recA RFLP analysis revealed unique B. dolosa restriction patterns. In addition, new 16S rDNA- and recA-based PCR assays allowed its specific identification.
Vermis
Karen
K
Laboratory for Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium.
Coenye
Tom
T
Laboratory for Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
LiPuma
John J
JJ
Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI 48109-0646, USA.
Mahenthiralingam
Eshwar
E
Cardiff School of Biosciences, PO Box 915, Cardiff University, Cardiff CF10 3TL, UK.
Nelis
Hans J
HJ
Laboratory for Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium.
Vandamme
Peter
P
Laboratory for Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
D001483:Base Sequence; D019117:Burkholderia / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D016956:Burkholderia cepacia / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D011938:Rec A Recombinases