HPLC-DAD-MS identification of bioactive secondary metabolites from Ferula communis roots.
A simple HPLC method was developed to distinguish between 'poisonous' and 'non-poisonous' chemotypes of Ferula communis. The method was performed on a C8 reverse phase analytical column using a binary eluent (aqueous TFA 0.01%-TFA 0.01% in acetonitrile) under gradient condition. The two chemotypes showed different fingerprints. The identification of five coumarins and eleven daucane derivatives by HPLC-diode array detection (HPLC-DAD) and HPLC-MS is described. A coumarin, not yet described, was detected.
Arnoldi
Lolita
L
Indena S.p.A. Research and Development Laboratories, Via Don Minzoni 6, 20090 Settala (MI), Italy. lolita.arnoldi@indena.com
Ballero
Mauro
M
Fuzzati
Nicola
N
Maxia
Andrea
A
Mercalli
Enrico
E
Pagni
Luca
L
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003374:Coumarins / Q000737:chemistry*; D005309:Ferula; D006801:Humans; D013058:Mass Spectrometry; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots; D017368:Protein Prenylation; D015203:Reproducibility of Results
D003374:Coumarins; D010936:Plant Extracts; C092340:ferulenol