Production of polyclonal antibodies against territrem B and detection of territrem B in the conidia of Aspergillus terreus 23-1 by immunoelectron microscopy.
Territrem B, a fungal metabolite isolated from Aspergillums terreus 23-1, is a tremorgenic mycotoxin. Immunoelectron microscopy using anti-territrem B polyclonal antibody was used to detect territrem B in the fungal body of A. terreus 23-1 at different times of culture without shaking on potato dextrose (PD) agar medium. The anti-territrem B serum was produced by immunization of rabbits with 4beta-hydroxymethyl-4beta-demethylterritrem B-sccinate bound by a linker to keyhole limpet hemocyanin. This antiserum recognized territrems and immunoelectron microscopy using this antiserum, and colloidal gold-conjugated anti-rabbit IgG antibodies showed that territrem B was localized to the fungal body of A. terreus 23-1. Territrem B was first seen in the cytoplasm of the conidia after 4 days' culture on PD agar medium. Maximal territrem B production in the conidia was seen on the 14th day of culture; however, territrem B was not formed in the hyphae at any stage of culture. These results are consistent with the previous finding that the formation of territrems is related to fungal sporulation.
Peng
Fu-Chuo
FC
Department of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei, Taiwan. fcpeng@ha.mc.ntu.edu.tw
Cheng
Chiung-Hsiang
CH
Liu
Jen-Cheng
JC
Wu
Ming-Fang
MF
Lee
Su-Huei
SH
Pan
Huei-Ju
HJ
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D001230:Aspergillus / Q000737:chemistry*; D006241:Haptens / Q000276:immunology; D007106:Immune Sera / Q000096:biosynthesis; D016253:Microscopy, Immunoelectron; D011714:Pyrans / Q000032:analysis* / Q000276:immunology*; D011817:Rabbits; D013172:Spores, Fungal / Q000737:chemistry
D000906:Antibodies; D006241:Haptens; D007106:Immune Sera; D011714:Pyrans; C040405:territrem B