title: Expression of reg I alpha protein in human gastric cancers.
abstract: OBJECTIVE
  Although regenerating gene(Reg) I alpha protein has a trophic effect on gastric epithelial cells, it is unclear whether Reg I alpha protein and its receptor are involved in gastric carcinogenesis. Therefore, we investigated the Reg I alpha protein expression in human gastric cancers and assessed its relationship to clinicopathological factors.
  
  
  METHODS
  Sixty-one gastric cancer specimens were examined, using immunohistochemistry, for Reg I alpha protein, p53, and proliferating cell nuclear antigen. The expression of both Reg I alpha and Reg receptor mRNA was examined in seven human gastric cancer cell lines (MKN1, MKN28, MKN45, MKN74, KATOIII, GCIY, and AGS) by reverse transcription-polymerase chain reaction and Northern blot analysis.
  
  
  RESULTS
  Twenty-three (37.7%) of the 61 gastric cancer tissues samples were positive for Reg I alpha protein. The Reg I alpha expression was significantly related to the presence of lymphatic invasion but not to tumor size, tumor stage, Lauren's classification, presence of venous invasion, lymph node metastases, or p53 overexpression. Gastric cancers positive for Reg I alpha protein showed a significantly higher proliferating cell nuclear antigen labeling index than negative ones. The expression of both Reg I alpha and Reg receptor mRNA was detected in all seven gastric cancer cell lines.
  
  
  CONCLUSIONS
  Reg I alpha protein may play a role in the development of gastric cancers.
authors.affiliation: Department of Gastroenterology and Hepatology, Kyoto University Graduate School of Medicine, Sakyo Kyoto, Japan. hfukui@kuhp.kyoto-u.ac.jp
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Fukui
authors.forename: Shigehiko
authors.initials: S
authors.lastname: Fujii
authors.forename: Jun
authors.initials: J
authors.lastname: Takeda
authors.forename: Takahisa
authors.initials: T
authors.lastname: Kayahara
authors.forename: Akira
authors.initials: A
authors.lastname: Sekikawa
authors.forename: Apichart
authors.initials: A
authors.lastname: Nanakin
authors.forename: Katsumasa
authors.initials: K
authors.lastname: Suzuki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hisatsune
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Seno
authors.forename: Mitsutaka
authors.initials: M
authors.lastname: Sawada
authors.forename: Takahiro
authors.initials: T
authors.lastname: Fujimori
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Chiba
mesh_terms: D002471:Cell Transformation, Neoplastic; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D037181:Lectins, C-Type / Q000096:biosynthesis; D051847:Lithostathine; D008297:Male; D008875:Middle Aged; D010189:Pancreatic Juice; D011498:Protein Precursors / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000503:physiopathology; D014407:Tumor Cells, Cultured
chemical_list: D006023:Glycoproteins; D037181:Lectins, C-Type; D051847:Lithostathine; D011498:Protein Precursors; C497617:REG1A protein, human; D012333:RNA, Messenger; C469954:Reg receptor, human