Establishing a selectivity survey as part of the method development activity for an isocratic reversed-phase liquid chromatographic method.
Often high-performance liquid chromatography method development is done by choosing a single C18 column and optimizing only the mobile phase composition. In this paper, it is demonstrated how to evaluate and optimize the best combination of the different stationary phase chemistries and mobile phases for a limited method development activity. By using column and mobile phase switching, it is possible to automate most of the activity in a nine-step process. Columns are chosen to represent the range of selectivity currently available. Interestingly, although the most popular column is the C18 phase, it is not the best column for the optimized methods in the cases studied.
Bidlingmeyer
Brian A
BA
Agilent Technologies, Inc., Little Falls Site, 2850 Centerville Road, Wilmington, DE 19808, USA.
Broske
Alan D
AD
Snyder
W Dale
WD