title: Amperometric enzyme electrodes for aerobic and anaerobic glucose monitoring prepared by glucose oxidase immobilized in mixed ferrocene-cobaltocenium dendrimers.
abstract: The enzyme glucose oxidase (GOx) has been immobilized electrostatically onto carbon and platinum electrodes modified with mixed ferrocene-cobaltocenium dendrimers. The ferrocene units have been used successfully as mediators between the GOx and the electrode under anaerobic conditions. In experiments carried out in the presence of oxygen, the cobaltocenium moieties act as electrocatalysts in the reduction of the oxygen in the solution, thus making possible the determination of the oxygen variation due to the enzymatic reaction, with high sensitivity. The current response of the electrode was determined by measuring steady-state current values obtained applying a constant potential. The effect of the substrate concentration, the dendrimer generation, the thickness of the dendrimer layer, interferences, and storage on the response of the sensors were investigated.
authors.affiliation: Departamento de Química Inorgánica, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain. beatriz.alonso@uam.es
authors.forename: Beatriz
authors.initials: B
authors.lastname: Alonso
authors.forename: Pilar García
authors.initials: PG
authors.lastname: Armada
authors.forename: José
authors.initials: J
authors.lastname: Losada
authors.forename: Isabel
authors.initials: I
authors.lastname: Cuadrado
authors.forename: Blanca
authors.initials: B
authors.lastname: González
authors.forename: Carmen M
authors.initials: CM
authors.lastname: Casado
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D003035:Cobalt / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis / Q000379:methods; D005296:Ferrous Compounds / Q000737:chemistry*; D005947:Glucose / Q000032:analysis* / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D008567:Membranes, Artificial; D000075163:Metallocenes; D010100:Oxygen / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; D008567:Membranes, Artificial; D000075163:Metallocenes; D011108:Polymers; D003035:Cobalt; D005949:Glucose Oxidase; D005947:Glucose; D010100:Oxygen; C004998:ferrocene