Isolation and characterization of two nuclear genes encoding glutathione and thioredoxin reductases from the yeast Kluyveromyces lactis.
Response to oxidative stress has been hitherto scarcely studied in the respiratory yeast Kluyveromyces lactis. The genes coding for reductases of glutathione and thioredoxin, KlGLR1 and KlTRR1, respectively, have been cloned and characterized in this work. H(2)O(2) treatment increased transcription and enzyme activity of KlTRR1 but not of KlGLR1, suggesting a different situation from that reported for the fermentative yeast Saccharomyces cerevisiae. A consensus for Yap1p binding is functional in the KlTRR1 promoter.
Tarrío
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Department of Molecular and Cell Biology, University of A Coruña, Campus da Zapateira s/n, 15071-A Coruña, Spain.
Díaz Prado
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Cerdán
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González Siso
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MI
D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000201:enzymology*; D003001:Cloning, Molecular; D005980:Glutathione Reductase / Q000737:chemistry / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007716:Kluyveromyces / Q000201:enzymology*; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013880:Thioredoxin-Disulfide Reductase / Q000737:chemistry / Q000235:genetics*; D014158:Transcription, Genetic
D006861:Hydrogen Peroxide; D005980:Glutathione Reductase; D013880:Thioredoxin-Disulfide Reductase