Fine-tuning of helper T cell activation and apoptosis by antigen-presenting cells.
The role of antigen-presenting cells (APC) in regulating helper T cell responses and activation-induced cell death (AICD) was investigated in vitro. T cell activation was monitored by measuring the early rise of intracellular free calcium [Ca+]ic, mRNA and cell surface expression of activation and apoptotic molecules, the production of cytokines and the activation of transcription factors. Our results demonstrate that the unique characteristics of a given APC can modify the threshold, kinetics and magnitude of the T cell response. The rapid and sustained rise of intracellular free calcium correlated well with the extent of cytokine production and the expression of activation molecules. Fas-dependent AICD could be induced by the most potent antigen-presenting cell (2PK3) only. Our results demonstrate that the response and fate of effector/memory CD4+ helper T lymphocytes is highly dependent on the individual properties of the APC they encounter.
Ludanyi
Katalin
K
Institute of Immunology, Medical and Health Science Center, University of Debrecen, 98 Nagyerdei Boulevard, Debrecen H-4012, Hungary.
Gogolak
Peter
P
Rethi
Bence
B
Magocsi
Maria
M
Detre
Cynthia
C
Matko
Janos
J
Rajnavolgyi
Eva
E
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D017209:Apoptosis / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002118:Calcium / Q000378:metabolism; D020169:Caspases / Q000378:metabolism; D016923:Cell Death / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D005434:Flow Cytometry; D051379:Mice; D014157:Transcription Factors / Q000378:metabolism
D016207:Cytokines; D014157:Transcription Factors; D020169:Caspases; D002118:Calcium