Intercalation behavior and tensile strength of DNA-lipid films for the dental application.
In this study, we prepared DNA-lipid films and examined their intercalation behavior and tensile strength as an indicator for usefulness as a dental material. The lipids were synthesized from the reaction of glycine, l-alanine, or l-glutamic acid with n-alkyl alcohol in the presence of p-toluenesulfonic acid. The self-standing, water-insoluble DNA-lipid films were prepared by casting the DNA-lipid complex from chloroform/ethanol solution. The DNA-lipid films formed intercalation complexes with ethidium bromide. This indicates that DNA-lipid films maintain a double helical structure. The tensile strengths of DNA films were 0.8-2.4MPa and were compatible with a commercially available material (Membrane) for guided tissue regeneration in dental use. We conclude that DNA-lipid films have potential for use as a material for the surface treatment of implanted materials or as a bone-guiding scaffold for dental application.
Fukushima
Tadao
T
Department of Dental Engineering, Biomaterials Section, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan. tadaof@college.fdcnet.ac.jp
Hayakawa
Tohru
T
Inoue
Yusuke
Y
Miyazaki
Koji
K
Okahata
Yoshio
Y
D004247:DNA / Q000737:chemistry*; D003764:Dental Materials; D004996:Ethidium / Q000737:chemistry; D007364:Intercalating Agents / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D013718:Tensile Strength
D003764:Dental Materials; D007364:Intercalating Agents; D008055:Lipids; D004247:DNA; D004996:Ethidium