title: Relationship between serum amyloid A level and Tanis/SelS mRNA expression in skeletal muscle and adipose tissue from healthy and type 2 diabetic subjects.
abstract: Tanis is a recently described protein reported to be a putative receptor for serum amyloid A and found to be dysregulated with diabetes in the Israeli sand rat Psamommys obesus. Tanis has also been identified as a selenoprotein, one of the first two identified membrane selenoproteins. We determined mRNA expression of the human homologue of Tanis, SelS/AD-015, in skeletal muscle and adipose tissue biopsies obtained from 10 type 2 diabetic patients and 11 age- and weight-matched healthy subjects. Expression of Tanis/SelS mRNA in skeletal muscle and adipose tissue biopsies was similar between diabetic and control subjects. A subset of subjects underwent a euglycemic-hyperinsulinemic clamp, and adipose tissue expression of Tanis/SelS was determined after in vivo insulin stimulation. Adipose tissue Tanis/SelS mRNA expression was unchanged after insulin infusion in control subjects, whereas Tanis/SelS mRNA increased in seven of eight subjects following insulin stimulation in diabetic subjects. Skeletal muscle and adipose tissue Tanis/SelS mRNA expression were positively correlated with plasma serum amyloid A. In conclusion, there is a strong trend toward upregulation of Tanis/SelS following insulin infusion in adipose tissue from type 2 diabetic subjects. Moreover, the positive relationship between Tanis mRNA and the acute-phase protein serum amyloid A suggests an interaction between innate immune system responses and Tanis expression in muscle and adipose tissue.
authors.affiliation: Integrative Physiology, Department of PhysiologyPharmacology, Karolinska Institutet, 171 77 Stockholm, Sweden.
authors.forename: Håkan K R
authors.initials: HK
authors.lastname: Karlsson
authors.forename: Hiroki
authors.initials: H
authors.lastname: Tsuchida
authors.forename: Staffan
authors.initials: S
authors.lastname: Lake
authors.forename: Heikki A
authors.initials: HA
authors.lastname: Koistinen
authors.forename: Anna
authors.initials: A
authors.lastname: Krook
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D015309:Glucose Clamp Technique; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051140:Selenoproteins; D000685:Serum Amyloid A Protein / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D007328:Insulin; D008565:Membrane Proteins; D012333:RNA, Messenger; C487710:SELENOS protein, human; D051140:Selenoproteins; D000685:Serum Amyloid A Protein