title: Leukocyte subsets and specific antibodies in pigs vaccinated with a classical swine fever subunit (E2) vaccine and the attenuated ORF virus strain D1701.
abstract: Total white blood cell (WBC) counts and percentages of CD4a+, CD8a+, CD5a+, CD45RA+, CD45RC+, wCD21+ and SWC3a+ cells in the peripheral blood of pigs were analysed in this study. Blood samples were collected before and on days 4, 10, 21 and 28 after vaccination. Group 1 pigs were vaccinated with a subunit E2 vaccine (gp E2 32 microg/dose), and Group 2 received a subunit vaccine combined with an attenuated ORF virus strain D1701 10(6.45) TCID50/dose. Control pigs received a placebo. The total WBC count and percentage of particular cell types were within the normal range in vaccinated and control pigs. Although the mechanism of attenuated ORF virus activity is not clear, changes were observed in CD4a+, CD5a+, CD8a+, CD45RA+ and CD45RC+ cells in pigs that received the combination of a subunit vaccine and ORF virus. However, the percentage of wCD21+ and SWC3a+ did not differ significantly from that recorded in pigs given only the subunit vaccine. At days 4 and 10 the number of pigs positive to E2 antibodies was higher in the group that received the subunit vaccine and ORF virus than in pigs vaccinated with the subunit vaccine only. A higher percentage of memory cells (CD45RC+) as well as Th and Tc lymphocytes in pigs that received the ORF virus and the subunit vaccine could be ascribed to a nonspecific influence of the ORF virus on the development (through cognate interactions between T and B cells) and the duration (presumed according to the finding of the clonal expression of memory cells) of humoral immunity (assessed by a higher number of seropositive pigs in this group). This seems likely since the proportion of these cells was found to be lower in the pigs that received E2 vaccine only.
authors.affiliation: Croatian Veterinary Institute, Savska cesta 143, PO Box 883, 10000 Zagreb, Croatia. terzic@veinst.hr
authors.forename: Svjetlana
authors.initials: S
authors.lastname: Terzić
authors.forename: Lorena
authors.initials: L
authors.lastname: Jemersić
authors.forename: M
authors.initials: M
authors.lastname: Lojkić
authors.forename: Lidija
authors.initials: L
authors.lastname: Sver
authors.forename: I
authors.initials: I
authors.lastname: Valpotić
authors.forename: Nada
authors.initials: N
authors.lastname: Orsolić
authors.forename: Andrea
authors.initials: A
authors.lastname: Humski
authors.forename: Z
authors.initials: Z
authors.lastname: Cvetnić
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D006691:Classical Swine Fever / Q000276:immunology* / Q000517:prevention & control*; D006692:Classical Swine Fever Virus / Q000145:classification / Q000276:immunology*; D005434:Flow Cytometry / Q000662:veterinary; D007958:Leukocyte Count / Q000662:veterinary; D016131:Lymphocyte Subsets; D009923:Orf virus / Q000145:classification / Q000276:immunology*; D013552:Swine; D016896:Treatment Outcome; D014611:Vaccination / Q000662:veterinary*; D014613:Vaccines, Attenuated / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D014613:Vaccines, Attenuated; D014765:Viral Vaccines