title: Detection of bone marrow infiltration of lymphoma cells by fluorescence in situ hybridization.
abstract: BACKGROUND
  It is sometimes difficult to detect the bone marrow infiltration of lymphoma cells, because lymphoma cells are not distinguishable from normal lymphocytes due to the similarity of their phenotype.
  
  
  METHODS
  Bone marrow involvement of 17 samples of 15 patients with follicular lymphoma, whose lymphoma cells were confirmed to harbor the translocation of chromosome14q32, were examined by microscopic analysis of bone marrow smear and biopsy, flow cytometorical analysis (FCM), chromosomal analysis of G-banding and fluorescence in situ hybridization (FISH). FISH was performed using a probe, which detects the split of IGH gene on 14q32.
  
  
  RESULTS
  The positivity of FISH was highest among these methods and FISH was able to detect the bone marrow involvement in one case who was defined as negative by bone marrow biopsy.
  
  
  CONCLUSIONS
  FISH can be used for detection of bone marrow involvement of malignant lymphoma that carries chromosomal rearrangement involving 14q32.
authors.affiliation: Department of Rheumatology and Hematology, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Ishizawa
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Obara
authors.forename: Osamu
authors.initials: O
authors.lastname: Seki
authors.forename: Shinichi
authors.initials: S
authors.lastname: Fujimaki
authors.forename: Junichi
authors.initials: J
authors.lastname: Kameoka
authors.forename: Ryo
authors.initials: R
authors.lastname: Ichinohasama
authors.forename: Keiji
authors.initials: K
authors.lastname: Kanemitsu
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sasaki
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Kaku
authors.forename: Hideo
authors.initials: H
authors.lastname: Harigae
mesh_terms: D001853:Bone Marrow / Q000473:pathology*; D001856:Bone Marrow Examination; D002871:Chromosome Banding; D002883:Chromosomes, Human, Pair 14; D005434:Flow Cytometry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008223:Lymphoma / Q000473:pathology*; D008224:Lymphoma, Follicular / Q000473:pathology; D009361:Neoplasm Invasiveness / Q000473:pathology; D014178:Translocation, Genetic