Screening for topoisomerase I binding compounds by high-performance liquid chromatography-mass spectrometry.
A new rapid compound screening approach for topoisomerase I binding activity is presented. DNA topoisomerase I is used as a target protein to capture binding compounds from a mixture of combinatorial compounds by bioaffinity ultrafiltration. Using high-performance liquid chromatography combined with electrospray ionization mass spectrometry, small-molecule active compounds were identified. We also have successfully applied this method to identifying compounds from cells grown in culture.
Zhang
Hong
H
Department of Food Science, Hangzhou University of Commerce, Hangzhou, People's Republic of China.
Gu
Qing
Q
Liang
Xinle
X
Pan
Yuanjiang
Y
D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004264:DNA Topoisomerases, Type I / Q000378:metabolism*; D008024:Ligands; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D008024:Ligands; D004264:DNA Topoisomerases, Type I