title: [Prokaryotic expression and bioactivity identification of Fab against human gamma-seminoprotein].
abstract: OBJECTIVE
  To construct the expression vector containing cDNA encoding Fab against human gamma-seminoprotein and express it in E. coli.
  
  
  METHODS
  The genes encoding K chain and Fd against gamma-seminoprotein were acquired from pUC19-K and pBluescript KS( M13-)-Fd by restrictive enzyme digestion and then cloned into the expression vector pComb3 to construct recombinant expression vector pComb3-Fab. pComb3-Fab was transfected into and expressed in XLI-Blue.
  
  
  RESULTS
  Fab against r-semino-protein was expressed in. XLI-Blue. Western blot analysis and immunocytochemical staining demonstrated that ex-pressed Fab could specifically bind to gamma-seminoprotein.
  
  
  CONCLUSIONS
  Fab against gamma -seminoprotein has been expressed successfully with biological activity, which create favourable condition for further study on targeted therapy of prostate cancer.
authors.affiliation: Department of Nephrology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. jifeng-sun@163.com
authors.forename: Ji-feng
authors.initials: JF
authors.lastname: Sun
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Xiao-ke
authors.initials: XK
authors.lastname: Hao
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Wei-hong
authors.initials: WH
authors.lastname: Wen
authors.forename: Ming
authors.initials: M
authors.lastname: Jin
authors.forename: Cheng-ji
authors.initials: CJ
authors.lastname: Wang
authors.forename: An-gang
authors.initials: AG
authors.lastname: Yang
mesh_terms: D000818:Animals; D006801:Humans; D015151:Immunoblotting; D007140:Immunoglobulin Fab Fragments / Q000032:analysis / Q000235:genetics*; D007150:Immunohistochemistry; D051379:Mice; D017430:Prostate-Specific Antigen / Q000276:immunology*
chemical_list: D007140:Immunoglobulin Fab Fragments; D017430:Prostate-Specific Antigen