Nucleosides and nucleotides. Part 226: alternate-strand triple-helix formation by 3'-3'-linked oligodeoxynucleotides composed of asymmetrical sequences.
In this paper, we describe the synthesis of the 3'-3'-linked oligonucleotides connected with pentaerythritol composed of asymmetrical sequences. Stability of the triplexes between these oligonucleotides and the DNA targets involving the adjacent oligopurine domains on alternate strands was investigated using the electrophoretic mobility shift assay (EMSA) and DNase I footprinting experiment. It was found that the 3'-3'-linked oligonucleotides composed of asymmetrical sequences formed the stable antiparallel triplexes with the DNA targets as compared with the unlinked oligonucleotides. Thus, oligonucleotides linked with pentaerythritol would be useful as antigene oligonucleotides for DNA targets consisting of the alternating oligopyrimidine-oligopurine sequences.
Hoshika
Shuichi
S
Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
Ueno
Yoshihito
Y
Kamiya
Hiroyuki
H
Matsuda
Akira
A
D001482:Base Composition; D001483:Base Sequence; D018983:DNA Footprinting; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry*; D009705:Nucleosides / Q000737:chemistry / Q000235:genetics; D009711:Nucleotides / Q000737:chemistry / Q000235:genetics; D009841:Oligonucleotides / Q000737:chemistry* / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods
D009692:Nucleic Acid Heteroduplexes; D009705:Nucleosides; D009711:Nucleotides; D009841:Oligonucleotides