Two novel antibiotics, Sch 419558 and Sch 419559, produced by Pseudomonas fluorescens: effect on activity by overexpression of RpoE.
Two new secondary metabolites designated as Sch 419558 (1) and Sch 419559 (2), were isolated from the fermentation broth of Pseudomonas fluorescens. Structure elucidation of 1 and 2 was accomplished by spectroscopic data analyses including MS and NMR experiments. Both compounds were identified as lipopeptides containing valine and threonine linked with 1-amino-1-hydroxy-heptadec-9-en-2-one or 1-amino-1-hydroxy-pentadecan-2-one carbon chains, respectively. Characterization of the amino acids was further confirmed by amino acid analysis. Compounds 1 and 2 exhibited antibacterial activity against a sensitized E. coli strain with minimum inhibitory concentration of 0.3 and 0.6 microg/mL, respectively. Overexpression of RpoE in the E. coli strain increased the MIC over 60-fold for compounds 1 and 2.
Yang
Shu-Wei
SW
Schering-Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA. shu-wei.yang@spcorp.com
Xu
Ling
L
Mierzwa
Ronald
R
He
Ling
L
Terracciano
Joseph
J
Patel
Mahesh
M
Gullo
Vincent
V
Black
Todd
T
Zhao
Wenjun
W
Chan
Tze-Ming
TM
Chu
Min
M
D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry* / Q000494:pharmacology*; D001426:Bacterial Proteins; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D015870:Gene Expression; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010455:Peptides; D011551:Pseudomonas fluorescens / Q000737:chemistry* / Q000378:metabolism; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D010455:Peptides; C495618:Sch 419558; C495617:Sch 419559; D012808:Sigma Factor; D014157:Transcription Factors; C063202:sporulation-specific sigma factors