Electron conduction across electrode-immobilized neutravidin bound with biotin-labeled ruthenium pentaamine.
Voltammetry is reported here of a self-assembled redox-protein conjugate consisting of neutravidin conjugated with a biotin derivative redox probe, Ru(NH3)5(N-[(N-[(4-pyridyl)methyl]biotinamide], immobilized on gold electrodes modified by self-assembled monolayers of mercaptoundecanoic acid. This voltammetry indicates that self-assembly of the conjugate/electrode electronic interface, driven by electrostatic binding between the monolayer and a single redox probe, favors orientation of the conjugate, resulting in electronic accessibility of the remaining three redox probes.
Jhaveri
Sulay D
SD
Nova Research, Inc, 1900 Elkin St, Alexandria, Virginia 22308, USA.
Trammell
Scott A
SA
Lowy
Daniel A
DA
Tender
Leonard M
LM
D000588:Amines / Q000737:chemistry* / Q000378:metabolism; D001360:Avidin / Q000737:chemistry* / Q000378:metabolism; D001710:Biotin / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D004583:Electrons; D008958:Models, Molecular; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry / Q000378:metabolism; D011422:Propionates / Q000737:chemistry; D012428:Ruthenium / Q000737:chemistry* / Q000378:metabolism; D013194:Staining and Labeling
D000588:Amines; D009942:Organometallic Compounds; D011422:Propionates; C093483:neutravidin; D001360:Avidin; D001710:Biotin; D012428:Ruthenium