title: Inactivation of Helicobacter pylori cagA gene affects motility.
abstract: BACKGROUND
  The cytotoxin-associated protein CagA is a Helicobacter pylori immunodominant antigen whose gene resides in the cag pathogenicity island. Our purpose was to determine if the disruption or deletion of cagA gene could have an effect on the expression of other proteins at the proteome level. We analyzed two H. pylori strains, 328 and G27 wild-type, bearing the cag pathogenicity island, and their respective isogenic cagA(-) mutants.
  
  
  METHODS
  The proteomes of two H. pylori strains (328 and its isogenic mutant SPM328_DeltacagA) were resolved by two-dimensional electrophoresis and the digitalized images obtained were analysed both quantitatively and qualitatively. Peculiar spots of each strain were identified by mass spectrometry or by Western blotting.
  
  
  RESULTS
  The comparison between the proteome expression of an H. pylori cagA(+) strain and an isogenic mutant strain where the cagA gene was disrupted showed that, as well as the lack of expression of CagA, both flagellin A and flagellin B expressions were significantly decreased. The cagA(-) isogenic mutant was nonmotile. G27_DeltacagA, in which CagA was inactivated by gene deletion, was nonmotile as well respecting to motile G27 wild-type strain. Moreover, reintroduction of cagA in G27_DeltacagA restored motility.
  
  
  CONCLUSIONS
  Our results suggest that CagA could quantitatively influence flaA and flaB transcription or their subsequent translation and/or correct folding.
authors.affiliation: Dipartimento di Medicina Interna, Scienze Endocrino-Metaboliche e Biochimica, Policlinico Le Scotte, 53100 Siena, Italy.
authors.forename: Natale
authors.initials: N
authors.lastname: Figura
authors.forename: Lorenza
authors.initials: L
authors.lastname: Trabalzini
authors.forename: Roberta
authors.initials: R
authors.lastname: Mini
authors.forename: Giulia
authors.initials: G
authors.lastname: Bernardini
authors.forename: Andrea
authors.initials: A
authors.lastname: Scaloni
authors.forename: Fabio
authors.initials: F
authors.lastname: Talamo
authors.forename: Paola
authors.initials: P
authors.lastname: Lusini
authors.forename: Elisa
authors.initials: E
authors.lastname: Ferro
authors.forename: Paola
authors.initials: P
authors.lastname: Martelli
authors.forename: Annalisa
authors.initials: A
authors.lastname: Santucci
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D004415:Dyspepsia / Q000382:microbiology; D015180:Electrophoresis, Gel, Two-Dimensional; D005408:Flagellin / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics / Q000502:physiology*; D006801:Humans; D009068:Movement; D020543:Proteome
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D020543:Proteome; C080866:cagA protein, Helicobacter pylori; D005408:Flagellin