Systemic bone formation with weekly PTH administration in ovariectomized rats.
OBJECTIVE
Weekly subcutaneous administration of 0 (vehicle), 10 and 80 microg/kg doses of human parathyroid hormone (1-34) [PTH (1-34)] were compared based on their capacity to induce systemic formation of bone in 9 month-old ovariectomized (OVX) Sprague-Dawley rats.


METHODS
Changes elicited at bone tissue after 4 weeks of treatment were assessed using dual x-ray absorptiometry, micro-computed tomography (microCT), and ashing.


RESULTS
The 10 microg/kg dose led to a significant increase (p<0.025) in femoral bone mineral density (BMD) over vehicle- and 80 microg/kg-treated groups. Similarly, structural analysis of the femoral neck trabecular bone by microCT revealed increases in bone volume fraction and trabecular thickness over the pre-treatment baseline, and vehicle- and 80 microg/kg-treated groups.


CONCLUSIONS
The data suggest that the weekly administration of 10 microg/kg of PTH (1-34) was sufficient to significantly promote the bone mineral density systemically. The weekly administration of 10 microg/kg over a 4-week treatment period is, to our knowledge, one of the lowest reported total dose of PTH (1-34) shown to induce a net anabolic effect on skeletal tissue in OVX rats.
Gittens
Sébastien A
SA
Faculty of Pharmacy & Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada
Wohl
Gregory R
GR
Zernicke
Ronald F
RF
Matyas
John R
JR
Morley
Paul
P
Uludag
Hasan
H
D000818:Animals; D015992:Body Mass Index; D015519:Bone Density / Q000187:drug effects*; D005260:Female; D008159:Lumbar Vertebrae / Q000033:anatomy & histology / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D010052:Ovariectomy; D010281:Parathyroid Hormone / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000601:surgery; D013977:Tibia / Q000737:chemistry / Q000187:drug effects
D010281:Parathyroid Hormone; D010446:Peptide Fragments