Differences in responsiveness of mouse strain against p-benzoquinone as assessed by non-radioisotopic murine local lymph node assay.
The non-radioisotopic modification of murine local lymph node assay (LLNA) by using 5-bromo-2'-deoxyuridine (BrdU) was conducted to investigate the strain-related difference of the responsiveness of mice to p-benzoquinone (PBQ) with BALB/cAnN, CBA/JN and CD-1 mouse strains. Strain and dose related differences were analyzed by two-way analysis of variance (two-way ANOVA). CBA/JN was considered to be the highest responsive strain to PBQ, and interaction was detected between CD-1 and each of the other inbred strains. These results support the recommendation in the OECD test guideline 429 and the skin sensitization test guideline of US-EPA with regard to the selection of mouse strain for LLNA.
Takeyoshi
Masahiro
M
Hita Laboratory, Chemicals Evaluation and Research Institute, Japan, Oita, Japan.
Noda
Shuji
S
Yamasaki
Kanji
K
D000704:Analysis of Variance; D000818:Animals; D016227:Benzoquinones / Q000633:toxicity*; D001973:Bromodeoxyuridine / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D023441:Local Lymph Node Assay; D008198:Lymph Nodes / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains / Q000378:metabolism*
D016227:Benzoquinones; C004532:quinone; D001973:Bromodeoxyuridine