[The study of primary culture methods for hippocampal neurons of newborn rats].
OBJECTIVE
To compare three different methods for neuron culture, so as to provide a culture technique with higher neuron purity and survival rate.


METHODS
Neurons in hippocampal region of newborn SD rats were cultured by common culture method, cytosine arabinoside (Ara-c)-supplementing method, Ara-c and nerve growth factor (NGF)-supplementing method. Then morphology of neurons was observed under microscope. The survival rates of the neurons at different culture times were compared by inverted microscope observation and MTT colorimetry. The purity of neurons cultured by 3 methods was detected by immunocytochemical staining.


RESULTS
Neurons cultured by Ara-c and NGF-supplementing method grew well. The purity and survival rate of neurons cultured by Ara-c and NGF-supplementing method was highest.


CONCLUSIONS
Ara-c and NGF-supplementing method is a good method for neuron culture.
Wang
Ying
Y
PLA Center of Neurology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Che
Yu
Y
Miao
Jian-Ting
JT
Li
Zhu-Yi
ZY
Wang
Zhe-Jin
ZJ
D000818:Animals; D002478:Cells, Cultured; D003561:Cytarabine; D006624:Hippocampus / Q000166:cytology; D009414:Nerve Growth Factors; D009474:Neurons / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley
D009414:Nerve Growth Factors; D003561:Cytarabine