title: Analytical performance of several classical swine fever laboratory diagnostic techniques on live animals for detection of infection.
abstract: The diagnostic properties of several assays on live animals were studied using data from different experiments. These experiments involved 128 classical swine fever virus (CSFV) infected pigs (weaner pigs, fatteners and sows). Since all pigs in the study were infected with CSFV, only the proportion of test positive results and the time until a test positive result is obtained were evaluated. The RT-nPCR detected the highest proportion of infected pigs (98.9%), whereas the Antigen ELISA gave the worst detection results (74.7%). Within the group of test positive animals, infection was detected earliest using the leukocyte count and latest using Antigen ELISA. Using the virus neutralisation test, antibodies against CSFV were detectable on average 7.6 days after the onset of viraemia in virus isolation in whole blood. Using survival analysis, the time until the first positive diagnosis and the proportion of detected animals were combined in one test. Results showed that RT-nPCR performed significantly better than either virus isolation in different blood fractions or antigen ELISA. It is concluded that the RT-nPCR technique is the best diagnostic tool available for early detection of a classical swine fever infection.
authors.affiliation: Department of Reproduction, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. jeroen.dewulf@ugent.be
authors.forename: Jeroen
authors.initials: J
authors.lastname: Dewulf
authors.forename: Frank
authors.initials: F
authors.lastname: Koenen
authors.forename: Koen
authors.initials: K
authors.lastname: Mintiens
authors.forename: Pierre
authors.initials: P
authors.lastname: Denis
authors.forename: Stefaan
authors.initials: S
authors.lastname: Ribbens
authors.forename: Aart
authors.initials: A
authors.lastname: de Kruif
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000097:blood; D006691:Classical Swine Fever / Q000175:diagnosis*; D006692:Classical Swine Fever Virus / Q000302:isolation & purification*; D019411:Clinical Laboratory Techniques / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013552:Swine
chemical_list: D000956:Antigens, Viral