title: Two novel antibiotics, Sch 419558 and Sch 419559, produced by Pseudomonas fluorescens: effect on activity by overexpression of RpoE.
abstract: Two new secondary metabolites designated as Sch 419558 (1) and Sch 419559 (2), were isolated from the fermentation broth of Pseudomonas fluorescens. Structure elucidation of 1 and 2 was accomplished by spectroscopic data analyses including MS and NMR experiments. Both compounds were identified as lipopeptides containing valine and threonine linked with 1-amino-1-hydroxy-heptadec-9-en-2-one or 1-amino-1-hydroxy-pentadecan-2-one carbon chains, respectively. Characterization of the amino acids was further confirmed by amino acid analysis. Compounds 1 and 2 exhibited antibacterial activity against a sensitized E. coli strain with minimum inhibitory concentration of 0.3 and 0.6 microg/mL, respectively. Overexpression of RpoE in the E. coli strain increased the MIC over 60-fold for compounds 1 and 2.
authors.affiliation: Schering-Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA. shu-wei.yang@spcorp.com
authors.forename: Shu-Wei
authors.initials: SW
authors.lastname: Yang
authors.forename: Ling
authors.initials: L
authors.lastname: Xu
authors.forename: Ronald
authors.initials: R
authors.lastname: Mierzwa
authors.forename: Ling
authors.initials: L
authors.lastname: He
authors.forename: Joseph
authors.initials: J
authors.lastname: Terracciano
authors.forename: Mahesh
authors.initials: M
authors.lastname: Patel
authors.forename: Vincent
authors.initials: V
authors.lastname: Gullo
authors.forename: Todd
authors.initials: T
authors.lastname: Black
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhao
authors.forename: Tze-Ming
authors.initials: TM
authors.lastname: Chan
authors.forename: Min
authors.initials: M
authors.lastname: Chu
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry* / Q000494:pharmacology*; D001426:Bacterial Proteins; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D015870:Gene Expression; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010455:Peptides; D011551:Pseudomonas fluorescens / Q000737:chemistry* / Q000378:metabolism; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D010455:Peptides; C495618:Sch 419558; C495617:Sch 419559; D012808:Sigma Factor; D014157:Transcription Factors; C063202:sporulation-specific sigma factors