[Experiment and study of endothelialization of prostheses with bone marrow CD34+ cells].
OBJECTIVE
To exploration the endothelialization of prostheses with bone marrow CD(34)(+) cells.


METHODS
CD(34)(+) cells were isolated from bone marrow of carine by an immune magnetic cell sorting system and induced into endothelial cells with VEGF. Seeding the cells to PTFE prostheses which implanted the abdominal aorta artery (AAA) and inferior vena cava (IVC).


RESULTS
The isolated cells from bone marrow were CD(34)(+) by flow cytometer which could differentiate into endothelial cells in vascular endothelial growth factor (VEGF). The endothelial cells were identified by immunostaining and transmission electron microscope. The obliteration rate of the seeding grafts implanting AAA was 0%, the stenosis rate 12.5%; the obliteration rate implanting IVC 12.5%, the stenosis rate 25%.


CONCLUSIONS
CD(34)(+) cells were isolated from bone marrow by an immune magnetic cell sorting system and were able to be induced into endothelial cells with VEGF in vitro. PTFE prostheses seeding CD(34)(+) cells have the ideal endothelialization and patency.
Zhang
Hong-kun
HK
Department of Vascular Surgery, The First Affiliated Hospital College of Medicine, Zhejiang University, HangZhou 310003, China.
Zheng
Shu-sen
SS
Wang
Zhong-gao
ZG
Li
Ming
M
Jin
Wei
W
Feng
Hua
H
Shan
Ping
P
Zhao
Hai-ge
HG
Chen
Xu-dong
XD
D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism*; D001807:Blood Vessel Prosthesis; D019917:Blood Vessel Prosthesis Implantation; D001853:Bone Marrow / Q000098:blood supply; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D004285:Dogs; D042783:Endothelial Cells / Q000187:drug effects / Q000648:ultrastructure; D005260:Female; D005434:Flow Cytometry; D008297:Male; D008854:Microscopy, Electron; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology
D018952:Antigens, CD34; D042461:Vascular Endothelial Growth Factor A