title: What can we learn from highly purified neutrophils?
abstract: Neutrophil purification has traditionally been performed by centrifugation of leucocytes through density gradients. These reliable methods produce populations that are typically >95% pure neutrophils, and have allowed the widespread study of the function of these cells. Our recent work has suggested that residual monocytes may play a more important role than has been previously realized, and suggest that for some functional experiments, further purification of cells is required to understand fully the neutrophil phenotype.
authors.affiliation: Division of Genomic Medicine, University of Sheffield, M Floor, Royal Hallamshire Hospital, Sheffield S10 2JF, UK.
authors.forename: I
authors.initials: I
authors.lastname: Sabroe
authors.forename: L R
authors.initials: LR
authors.lastname: Prince
authors.forename: S K
authors.initials: SK
authors.lastname: Dower
authors.forename: S R
authors.initials: SR
authors.lastname: Walmsley
authors.forename: E R
authors.initials: ER
authors.lastname: Chilvers
authors.forename: M K B
authors.initials: MK
authors.lastname: Whyte
mesh_terms: D000818:Animals; D017209:Apoptosis; D002469:Cell Separation; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D010641:Phenotype; D013997:Time Factors
chemical_list: D008070:Lipopolysaccharides