title: Toxic effects of bis(thiosemicarbazone) compounds and its palladium(II) complexes on herpes simplex virus growth.
abstract: Here, we present data on the activity of benzyl bis(thiosemicarbazone); 3,5-diacyl-1,2,4-triazole bis(4-methylthiosemicarbazone) and their Pd(II) complexes against the replication of wild type and of acyclovir (ACV)-resistant, herpes simplex virus type 1 (HSV 1) and type 2 (HSV 2) strains. The data were compared to those under the action of acyclovir. The testing of cytotoxic activity suggests that these compounds may be endowed with important antiviral properties. It is interesting to note that the Pd(II)-benzyl bis(thiosemicarbazone) complex, 2, exhibits a significant activity against acyclovir-resistant viruses R-100 (HSV 1) and PU (HSV 2) with an in vitro selectivity index (SI) of 8.0 vs. 0.01 for acyclovir. This complex also negatively influenced the expression of key structural HSV 1 proteins (VP23, gH and gG/gD), thus suppressing simultaneously virus entry, transactivation of virus genome, capsid assembly, and cell-to-cell spread of infectious HSV progeny.
authors.affiliation: Laboratory of Virology, Faculty of Biology, Sofia University, 1164 Sofia, Bulgaria.
authors.forename: Petia
authors.initials: P
authors.lastname: Genova
authors.forename: Tatiana
authors.initials: T
authors.lastname: Varadinova
authors.forename: Ana I
authors.initials: AI
authors.lastname: Matesanz
authors.forename: Desislava
authors.initials: D
authors.lastname: Marinova
authors.forename: Pilar
authors.initials: P
authors.lastname: Souza
mesh_terms: D000212:Acyclovir / Q000494:pharmacology; D000818:Animals; D000998:Antiviral Agents; D015153:Blotting, Western; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004285:Dogs; D024882:Drug Resistance, Viral; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D018258:Herpesvirus 2, Human / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D009942:Organometallic Compounds / Q000494:pharmacology*; D010165:Palladium / Q000494:pharmacology*; D013882:Thiosemicarbazones / Q000494:pharmacology*; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D009942:Organometallic Compounds; D013882:Thiosemicarbazones; D014764:Viral Proteins; D010165:Palladium; D000212:Acyclovir