Experimental immunisation and protection of guinea pigs with Vibrio cholerae toxoid and mucinases, neuraminidase and proteinase.
As measured by fluid accumulation in ileal loops, Vibrio cholerae mucinase complex, with or without toxoid, protected guinea pigs from challenges with V. cholerae live organisms and enterotoxin. The neuraminidase and proteinases of the complex were combined in modified oil emulsion or aluminum hydroxide adjuvants and the resultant vaccines given by the parenteral or oral routes. There was little difference between the two types of adjuvant. Control of stomach acidity improved oral vaccination. Animals injected intramuscularly (i.m.) with toxoid-containing vaccines were protected from challenge with cholera toxin (CT) whereas those given oral doses were not. Toxoid plus killed V. cholerae cells elicited a more effective protection against toxin challenge than killed V. cholerae cells alone. Vaccines containing mucinases, with or without toxoid, protected the animals from a live V. cholerae challenge. The anti-mucinase immune response may prevent adhesion of the V. cholerae cells and hence reduce delivery of toxin to receptors. These mucinases, neuraminidase and proteinases, may be useful components of acellular, toxoided cholera vaccines for human immunisation.
Stewart-Tull
Duncan E S
DE
Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK.
Lucas
Carol
C
Bleakley
Carol R
CR
D000276:Adjuvants, Immunologic; D000284:Administration, Oral; D000536:Aluminum Hydroxide / Q000276:immunology; D000818:Animals; D002771:Cholera / Q000517:prevention & control*; D002772:Cholera Toxin / Q000276:immunology* / Q000633:toxicity; D022121:Cholera Vaccines / Q000008:administration & dosage / Q000276:immunology*; D005620:Freund's Adjuvant; D006168:Guinea Pigs; D007082:Ileum / Q000473:pathology; D007273:Injections, Intramuscular; D009439:Neuraminidase / Q000276:immunology; D010447:Peptide Hydrolases / Q000276:immunology; D011133:Polysaccharide-Lyases / Q000276:immunology; D014121:Toxoids / Q000276:immunology*; D014611:Vaccination; D015164:Vaccines, Inactivated / Q000276:immunology; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology; D014734:Vibrio cholerae / Q000276:immunology / Q000472:pathogenicity
D000276:Adjuvants, Immunologic; D022121:Cholera Vaccines; D014121:Toxoids; D015164:Vaccines, Inactivated; D022223:Vaccines, Subunit; C038458:cholera toxoid; D000536:Aluminum Hydroxide; D005620:Freund's Adjuvant; D002772:Cholera Toxin; D009439:Neuraminidase; D010447:Peptide Hydrolases; D011133:Polysaccharide-Lyases; C100002:hyaluronate lyase