title: Gene expression signatures in chronic and aggressive periodontitis: a pilot study.
abstract: This pilot study examined gene expression signatures in pathological gingival tissues of subjects with chronic or aggressive periodontitis, and explored whether new subclasses of periodontitis can be identified based on gene expression profiles. A total of 14 patients, seven with chronic and seven with aggressive periodontitis, were examined with respect to clinical periodontal status, composition of subgingival bacterial plaque assessed by checkerboard hybridizations, and levels of serum IgG antibodies to periodontal bacteria assayed by checkerboard immunoblotting. In addition, at least two pathological pockets/patient were biopsied, processed for RNA extraction, amplification and labeling, and used to study gene expression using Affymetrix U-133 A arrays. Based on a total of 35 microarrays, no significantly different gene expression profiles appeared to emerge between chronic and aggressive periodontitis. However, a de novo grouping of the 14 subjects into two fairly robust clusters was possible based on similarities in gene expression. These two groups had similar clinical periodontal status and subgingival bacterial profiles, but differed significantly with respect to serum IgG levels against the important periodontal pathogens Porphyromonas gingivalis, Tannerella forsythensis and Campylobacter rectus. These early data point to the usefulness of gene expression profiling techniques in the identification of subclasses of periodontitis with common pathobiology.
authors.affiliation: Division of Periodontics, Section of Oral and Diagnostic Sciences, School of Dental and Oral Surgery, Columbia University, New York, NY 10032, USA. pp192@columbia.edu
authors.forename: Panos N
authors.initials: PN
authors.lastname: Papapanou
authors.forename: Armin
authors.initials: A
authors.lastname: Abron
authors.forename: Miguel
authors.initials: M
authors.lastname: Verbitsky
authors.forename: Doros
authors.initials: D
authors.lastname: Picolos
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Qin
authors.forename: James B
authors.initials: JB
authors.lastname: Fine
authors.forename: Paul
authors.initials: P
authors.lastname: Pavlidis
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics; D010520:Aggressive Periodontitis / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D000907:Antibodies, Bacterial / Q000235:genetics; D001439:Bacteroides / Q000235:genetics; D044883:Campylobacter rectus / Q000235:genetics; D002908:Chronic Disease; D004269:DNA, Bacterial / Q000032:analysis; D003773:Dental Plaque / Q000382:microbiology; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000235:genetics; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D010518:Periodontitis / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*; D010865:Pilot Projects; D016966:Porphyromonas gingivalis / Q000235:genetics
chemical_list: D000907:Antibodies, Bacterial; D004269:DNA, Bacterial; D007074:Immunoglobulin G