Different frequency treadmill running in immobilization-induced muscle atrophy and ankle joint contracture of rats.
We investigated the effects of different frequencies of treadmill running on immobilization-induced soleus and gastrocnemius muscle atrophy and ankle joint contracture in rats using morphology and histochemistry. The right ankle joint of rat was immobilized for 2 weeks. Thereafter, the rats were randomly assigned to four groups for 6 weeks of exercise under different conditions: free cage activity and free remobilization (FR), once-a-week treadmill running (low-frequency running program (LFR)), three-time-a-week running (middle-frequency running program (MFR)), and six-time-a-week running (high-frequency running program (HFR)) groups. Two weeks of immobilization significantly reduced the cross-sectional area of soleus type I (62%, P<0.05) and type II muscle fibers (66%, P<0.05), gastrocnemius type I (78%, P<0.05) and type II muscle fibers (68%, P<0.05), and the range of ankle joint movement (46%, P<0.05). Immobilization also increased the ratio of type II to total fiber numbers in the soleus (P<0.05), and gastrocnemius (P<0.05), and induced pathological changes in muscle fibers. Some of these changes could not be corrected by free remobilization; however, the LFR, MFR, and HFR groups clearly recovered toward normal levels with exercise frequency, the effect on muscle recovery being more beneficial in the MFR and HFR groups. In addition, the range of ankle joint contracture was improved in LFR, MFR, and HFR groups in comparison with that in the FR group. These findings indicate that treadmill running exercise improved the immobilization-induced muscle fiber histochemical alterations and the range of the ankle motion in rats. Running three times and six times a week was more beneficial for recovery of immobilization-induced muscle atrophy and joint contracture compared with no running or once-a-week running.
Sakakima
Harutoshi
H
School of Health Sciences, Faculty of Medicine, Kagoshima University, 8-35-1, Sakuragaoka, Kagoshima 890-8520, Japan. sakaki@health.nop.kagoshima-u.ac.jp
Yoshida
Yoshihiro
Y
Sakae
Kiyohiro
K
Morimoto
Norio
N
D000818:Animals; D000843:Ankle Joint / Q000473:pathology*; D003286:Contracture / Q000209:etiology*; D005260:Female; D019417:Hindlimb Suspension; D018485:Muscle Fibers, Skeletal / Q000473:pathology; D009133:Muscular Atrophy / Q000209:etiology*; D051381:Rats; D017208:Rats, Wistar; D012420:Running