[Dynamic changes in the expressions of myogenic regulatory factors MyoD and myogenin during repair of muscle injury].
OBJECTIVE
To observe the dynamic changes in the expressions of myogenic regulatory factors MyoD and myogenin during the repair of injured muscle.


METHODS
Muscular injury model was established by local injection of bupivacain, and at different time points following the injection, the gastrocnemius muscles were collected for preparation of cryosections. HE staining was performed for examination of the pathological changes in the injured muscles, and the expressions of MyoD and myogenin were detected by SABC.


RESULTS
MyoD-positive nuclei began to appear 18 h after muscle injury, reaching the peak till 48 h after the injury. Myogenin-positive nuclei appeared 24 h after muscle injury and peaked at 72 h.


CONCLUSIONS
MyoD and myogenin play a role in muscle regeneration after muscle injury, and they may serve as the indexes to identify muscle precursor cells and mark muscle regeneration process.
Zeng
Ying
Y
Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. drzengying@21cn.com
Zhang
Cheng
C
Liu
Ke-xuan
KX
Li
Cai-ming
CM
Feng
San-wei
SW
Li
Qun
Q
Liu
Tai-yun
TY
Huang
Wen
W
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002045:Bupivacaine / Q000633:toxicity*; D004268:DNA-Binding Proteins / Q000032:analysis; D064326:MEF2 Transcription Factors; D009132:Muscles / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology; D017570:MyoD Protein / Q000032:analysis*; D018006:Myogenic Regulatory Factors; D018008:Myogenin / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration; D014157:Transcription Factors / Q000032:analysis
D004268:DNA-Binding Proteins; D064326:MEF2 Transcription Factors; D017570:MyoD Protein; C503803:Myog protein, rat; D018006:Myogenic Regulatory Factors; D018008:Myogenin; D014157:Transcription Factors; D002045:Bupivacaine