Heterogeneity of mitochondrial creatine kinase.
The heterogeneity of cardiac sarcomeric mitochondrial creatine kinase (creatine N-phosphotransferase, EC 2.7.3.2, sMi-CK), namely, brain ubiquitous Mi-CK (uMi-CK) and an atypical Mi-CK detected in the serum of a patient with ovarian cancer, was studied by isoelectric focusing. These Mi-CKs were found to be slightly different from each other with respect to their pIs under the examined conditions. The atypical Mi-CK was found to be an atypically oxidized form of uMi-CK. Results suggest that these heterogeneities of Mi-CK are caused by the genotypes, structures, biological functions and metabolism/dissimilation of Mi-CKs in the mitochondria and intravascular circulation.
Kanemitsu
Fusae
F
Clinical Laboratories, Kurashiki Central Hospital, 1-1-1 Miwa, Kurashiki 710-8602, Japan. fkl306@kchnet.or.jp
Kageoka
Takeshi
T
Kira
Shohei
S
D003402:Creatine Kinase / Q000378:metabolism*; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D008931:Mitochondria, Muscle / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology; D010051:Ovarian Neoplasms / Q000097:blood / Q000201:enzymology
D003402:Creatine Kinase