title: On-line coupling of flow injection displacement sorption preconcentration to high-performance liquid chromatography for speciation analysis of mercury in seafood.
abstract: A simple and cost-effective method for speciation analysis of trace mercury in seafood was developed by on-line coupling flow injection microcolumn displacement sorption preconcentration to high-performance liquid chromatography (HPLC) with UV detection. The methodology involved the presorption of the Cu-PDC (pyrrolidine dithiocarbamate) chelate onto a microcolumn packed with a cigarette filter sorbent, simultaneous preconcentration of Hg(II), methylmercury (MeHg), ethylmercury (EtHg), and phenylmercury (PhHg) onto the microcolumn through a displacement reaction with the presorbed Cu-PDC, and their subsequent elution from the microcolumn for on-line HPLC separation. Interferences from heavy metal ions with lower stability of their PDC chelates relative to Cu-PDC were minimized without the need of any masking agents. With the consumption of 4.0 ml of sample solution, the enrichment factors were about 80. The detection limits were 10-25 ng g(-1) (as Hg) in fresh tissue. Precision (R.S.D. (%), n = 5) ranged from 2 to 3% at the 500 microg l(-1) (as Hg) level. The developed technique was validated by analyzing a certified reference material (DORM-2, dogfish-muscle), and was shown to be useful for mercury speciation in real seafood samples.
authors.affiliation: State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, and Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Dong
authors.forename: Xiu-Ping
authors.initials: XP
authors.lastname: Yan
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
authors.forename: Shan-Wei
authors.initials: SW
authors.lastname: Wang
authors.forename: Dong-Qing
authors.initials: DQ
authors.lastname: Jiang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017022:Flow Injection Analysis / Q000379:methods*; D008628:Mercury / Q000737:chemistry*; D015203:Reproducibility of Results; D017747:Seafood / Q000032:analysis*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D008628:Mercury