Chromosomal transformation in donor cells following allogeneic bone marrow transplantation.
We report here a monosomy 7 transformation of donor cells following matched-unrelated, same sex, allogeneic bone marrow transplantation in a patient with severe congenital aplastic anemia. A PCR technique was employed to amplify microsatellite markers on chromosome 7 to confirm donor/recipient identity. We found that the transformation of monosomy 7 occurred in previously genetically normal donor cells. This study suggests that the microenvironment of the bone marrow of our patient with severe congenital aplastic anemia may have played a critical role in the development of monosomy 7 of normal donor cells and we conclude that chromosomal microsatellite marker analysis can be a valuable tool for precise donor/recipient differentiation in engraftment monitoring.
Lang
Z
Z
Center for Cancer and Blood Disorders, Washington, DC, USA.
Dinndorf
P
P
Ladisch
S
S
Bayever
E
E
Reaman
G
G
D000741:Anemia, Aplastic / Q000150:complications / Q000151:congenital / Q000628:therapy*; D016026:Bone Marrow Transplantation; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002897:Chromosomes, Human, Pair 7; D020732:Cytogenetic Analysis; D005260:Female; D006801:Humans; D007223:Infant; D009006:Monosomy; D016133:Polymerase Chain Reaction; D014019:Tissue Donors; D014184:Transplantation, Homologous