title: Methylated DNA collected by tampons--a new tool to detect endometrial cancer.
abstract: This proof of principle study aimed to define a new and simple strategy for detection of endometrial cancer using epigenetic markers. We investigated DNA isolated from vaginal secretion collected from tampon for aberrant methylation of five genes (CDH13, HSPA2, MLH1, RASSF1A, and SOCS2) using MethyLight in 15 patients with endometrial cancer and 109 patients without endometrial cancer. All endometrial cancer patients revealed three or more methylated genes, whereas 91% (99 of 109) of the patients without endometrial cancer had no or fewer than three genes methylated in their vaginal secretion. The methods developed in this study provide the basis for a prospective clinical trial to screen asymptomatic women who are at high risk for endometrial cancer.
authors.affiliation: Department of Obstetrics and Gynecology, Innsbruck University Hospital, Anichstrasse 35, A-6020 Innsbruck, Austria.
authors.forename: Heidi
authors.initials: H
authors.lastname: Fiegl
authors.forename: Conny
authors.initials: C
authors.lastname: Gattringer
authors.forename: Andreas
authors.initials: A
authors.lastname: Widschwendter
authors.forename: Alois
authors.initials: A
authors.lastname: Schneitter
authors.forename: Angela
authors.initials: A
authors.lastname: Ramoni
authors.forename: Daniela
authors.initials: D
authors.lastname: Sarlay
authors.forename: Inge
authors.initials: I
authors.lastname: Gaugg
authors.forename: Georg
authors.initials: G
authors.lastname: Goebel
authors.forename: Hannes M
authors.initials: HM
authors.lastname: Müller
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Mueller-Holzner
authors.forename: Christian
authors.initials: C
authors.lastname: Marth
authors.forename: Martin
authors.initials: M
authors.lastname: Widschwendter
mesh_terms: D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics; D016022:Case-Control Studies; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000032:analysis*; D016889:Endometrial Neoplasms / Q000175:diagnosis*; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000032:analysis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011336:Probability; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D013630:Tampons, Surgical; D014626:Vaginal Smears
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins