[Change of activity of NF-kappaB p65 in lymphocytes from rats with oral tolerance induced by Fx1A].
OBJECTIVE
To explore the activity change and significance of NF-kappaB p65 in lymphocytes from rats with oral tolerance induced by Fx1A.


METHODS
30 female Wistar rats were divided into two groups (oral tolerance and control groups), which stomachs were perfused with Fx1A and PBS, respectively. The delayed-type hypersensitivity (DTH) was evaluated by skin test, the immune function was detected by splenic lymphocyte proliferation test, the activity of NF-kappaB p65 and expression of TGF-beta1 in lymphoid tissues were examined by immunohistochemical staining and Sandwich ELISA.


RESULTS
In comparison with control group, the DTH and antigen-specific lymphocyte proliferation reaction in oral tolerance group were suppressed significantly. The activity of NF-kappaB p65 and expression of TGF-beta1 in Peyer's patch were increased notably. However, the activity of NF-kappaB p65 was decreased, but expression of TGF-beta1 was increased markedly in splenic lymphocytes.


CONCLUSIONS
Information of oral tolerance induced by Fx1A may be related to the change of NF-kappaB p65 activity in lymphocytes of body's different places.
Du
Xiao-gang
XG
Department of Nephrology, The First Affiliated Hospital of Chongqing University of Medical Science, Chongqing 400016, China.
Gan
Hua
H
Chen
Xue-mei
XM
D000284:Administration, Oral; D000818:Animals; D005260:Female; D026541:Heymann Nephritis Antigenic Complex / Q000008:administration & dosage*; D006968:Hypersensitivity, Delayed / Q000209:etiology; D007108:Immune Tolerance; D007150:Immunohistochemistry; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000737:chemistry*; D016328:NF-kappa B / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D051996:Transcription Factor RelA; D016212:Transforming Growth Factor beta / Q000032:analysis; D053773:Transforming Growth Factor beta1
D026541:Heymann Nephritis Antigenic Complex; D016328:NF-kappa B; C508962:Tgfb1 protein, rat; D051996:Transcription Factor RelA; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1