title: Influence of origin of isolates, especially endocarditis isolates, and various genes on biofilm formation by Enterococcus faecalis.
abstract: Endocarditis isolates of Enterococcus faecalis produced biofilm significantly more often than nonendocarditis isolates, and 39% of 79 versus 6% of 84 isolates produced strong biofilm (P < 0.0001). esp was not required, but its presence was associated with higher amounts of biofilm (P < 0.001). Mutants disrupted in dltA, efaA, ace, lsa, and six two-component regulatory systems were largely unaltered, while disruptions in epa (encoding enterococcal polysaccharide antigen), atn (encoding an autolysin), gelE (encoding gelatinase), and fsr (encoding the E. faecalis regulator) [corrected] resulted in fewer attached bacteria, as determined using phase-contrast microscopy, and less biofilm (P < 0.0001).
authors.affiliation: Division of Infectious Diseases, Department of Internal Medicine, University of Texas Medical School, Houston, Texas 77030, USA.
authors.forename: Jamal A
authors.initials: JA
authors.lastname: Mohamed
authors.forename: Wenxiang
authors.initials: W
authors.lastname: Huang
authors.forename: Sreedhar R
authors.initials: SR
authors.lastname: Nallapareddy
authors.forename: Fang
authors.initials: F
authors.lastname: Teng
authors.forename: Barbara E
authors.initials: BE
authors.lastname: Murray
mesh_terms: D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms; D004697:Endocarditis, Bacterial / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D018093:Gelatinases / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D009154:Mutation; D011137:Polystyrenes
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D011137:Polystyrenes; C116707:enterococcal surface protein, esp; D018093:Gelatinases