Structural and sequence comparisons arising from the solution structure of the transcription elongation factor NusG from Thermus thermophilus.
NusG is an essential bacterial protein modulator of transcriptional elongation and termination events, and interacts directly with RNA polymerase and Rho protein. Found also in Archaea, NusG shows stretches of sequence similarity to the eukaryotic transcription elongation factor Spt5. Herein, the three-dimensional solution structure of the bacterial NusG from Thermus thermophilus, which shows 43% amino acid sequence similarity to the Escherichia coli NusG, is described, and a survey of NusG and Spt5 amino acid sequences is presented. Although there is a clear evolutionary and functional relationship between these proteins, it is evident from the structural, sequence, and biochemical data that their binding specificities to both nucleic acids and other proteins differ.
Reay
Paul
P
Age Dimension Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
Yamasaki
Kazuhiko
K
Terada
Takaho
T
Kuramitsu
Seiki
S
Shirouzu
Mikako
M
Yokoyama
Shigeyuki
S
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D002868:Chromosomal Proteins, Non-Histone; D029968:Escherichia coli Proteins / Q000737:chemistry; D019614:Haloarcula marismortui / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010445:Peptide Elongation Factors / Q000737:chemistry*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012269:Ribosomal Proteins / Q000737:chemistry; D016415:Sequence Alignment; D017385:Sequence Homology; D012996:Solutions; D020349:Surface Plasmon Resonance; D013816:Thermodynamics; D016963:Thermus thermophilus / Q000737:chemistry*; D014157:Transcription Factors / Q000737:chemistry*; D035602:Transcriptional Elongation Factors
D001426:Bacterial Proteins; D002868:Chromosomal Proteins, Non-Histone; D029968:Escherichia coli Proteins; C070291:NusG protein, E coli; D010445:Peptide Elongation Factors; D012269:Ribosomal Proteins; D012996:Solutions; D014157:Transcription Factors; D035602:Transcriptional Elongation Factors; C036298:ribosomal protein L24; C069940:SPT5 transcriptional elongation factor; C078391:nusG protein, Thermus thermophilus