title: Sequence variation in pertussis S1 subunit toxin and pertussis genes in Bordetella pertussis strains used for the whole-cell pertussis vaccine produced in Poland since 1960: efficiency of the DTwP vaccine-induced immunity against currently circulating B. pertussis isolates.
abstract: The present study indicates that the appearance of the B. pertussis harbouring prn2 gene allele variant (not found among clinical isolates before 1990s) may have been induced by long-term vaccination in Poland with DTP-composed vaccine strains presenting exclusively prn1. However, ptxS1A allele of pertussis toxin subunit S1 encoding gene, predominant in the currently isolated B. pertussis strains, has been found in vaccine strains used for whole-cell pertussis component (wP) production of DTP vaccine in 1960-1978. This outrules the possibility that the appearance of ptxSIA allele might be related to vaccine pressure driven by non-ptxS1A vaccine strains used for long-term immunization with wP. Intranasal challenge animal model testing the efficiency of the clearance of B. pertussis strains harbouring different ptxS1/prn allele gene combinations revealed that currently produced DTwP vaccine may not contain adequate B. pertussis vaccine strains, since isolates with gene variants different from those observed in vaccine strains were eliminated from the lungs of the immunized animals with lower efficiency.
authors.affiliation: Department of Sera and Vaccines Evaluation, National Institute of Hygiene, Chocimska Street 24, 00-791 Warsaw, Poland. agzyl@pzh.gov.pl
authors.forename: Anna
authors.initials: A
authors.lastname: Gzyl
authors.forename: Ewa
authors.initials: E
authors.lastname: Augustynowicz
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Gniadek
authors.forename: Daniel
authors.initials: D
authors.lastname: Rabczenko
authors.forename: Grazyna
authors.initials: G
authors.lastname: Dulny
authors.forename: Janusz
authors.initials: J
authors.lastname: Slusarczyk
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D001886:Bordetella pertussis / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D015169:Colony Count, Microbial; D015721:Diphtheria-Tetanus-Pertussis Vaccine / Q000235:genetics* / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D005798:Genes, Bacterial; D008168:Lung / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D037342:Pertussis Toxin / Q000235:genetics* / Q000276:immunology; D011044:Poland; D016896:Treatment Outcome; D014611:Vaccination; D010566:Virulence Factors, Bordetella / Q000235:genetics* / Q000276:immunology; D014917:Whooping Cough / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D010566:Virulence Factors, Bordetella; C083388:pertussis toxin, S1 subunit; C068929:pertactin; D037342:Pertussis Toxin