title: Platelet aggregation and antibacterial effects of an l-amino acid oxidase purified from Bothrops alternatus snake venom.
abstract: The isolation and biochemical/enzymatic characterization of an L-amino acid oxidase, Balt-LAAO-I, from Bothrops alternatus snake venom, is described. Balt-LAAO-I is an acidic glycoprotein, pI approximately 5.37, homodimeric, Mr approximately 123,000, whose N-terminal sequence is ADVRNPLE EFRETDYEVL. It displays a high specificity toward hydrophobic and basic amino acids, while deglycosylation does not alter its enzymatic activity. Balt-LAAO-I induces platelet aggregation and shows bactericidal activity against Escherichia coli and Staphylococcus aureus. In addition, this enzyme is slightly hemorrhagic and induces edema in the mouse paw. Balt-LAAO-I is a multifunctional enzyme with promising relevant biotechnological and medical applications.
authors.affiliation: Laboratório de Bioquímica do Instituto de Pesquisas em Patologias Tropicais, Porto Velho-RO, Brazil.
authors.forename: Rodrigo G
authors.initials: RG
authors.lastname: Stábeli
authors.forename: Silvana
authors.initials: S
authors.lastname: Marcussi
authors.forename: Guilherme B
authors.initials: GB
authors.lastname: Carlos
authors.forename: Rosemeire C L R
authors.initials: RC
authors.lastname: Pietro
authors.forename: Heloísa S
authors.initials: HS
authors.lastname: Selistre-de-Araújo
authors.forename: José R
authors.initials: JR
authors.lastname: Giglio
authors.forename: Eduardo B
authors.initials: EB
authors.lastname: Oliveira
authors.forename: Andreimar M
authors.initials: AM
authors.lastname: Soares
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D017837:Bothrops; D003435:Crotalid Venoms / Q000201:enzymology*; D050830:L-Amino Acid Oxidase; D051379:Mice; D008969:Molecular Sequence Data; D010974:Platelet Aggregation / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D003435:Crotalid Venoms; D000594:Amino Acid Oxidoreductases; D050830:L-Amino Acid Oxidase