title: "Multiplex PCR" identification of the atypical and monophasic Salmonella enterica subsp. enterica serotype 1,4,[5],12:i:- in São Paulo State, Brazil: frequency and antibiotic resistance patterns.
abstract: Salmonella spp. are the etiologic agents of salmonellosis, a worldwide spread zoonoses causing foodborne outbreaks and clinical diseases. By serological identification, Salmonella enterica subsp. enterica serotype 1,4,[5],12:i:- accounted for 8.8% of human and 1.6% of nonhuman Salmonella strains isolated in São Paulo State, during 1991-2000. A total of 28.6% of them amplified a fragment corresponding to H:1,2 (flagellar phase two) through PCR analysis and were further assigned as S. Typhimurium. Antimicrobial resistance was detected in 36.3% of the 369 PCR-negative strains tested, including the multiresistance to ampicillin, chloramphenicol, sulfonamides, tetracycline, and streptomycin.
authors.affiliation: Enteropathogens Laboratory, Instituto Adolfo Lutz, São Paulo, Brazil. atavecch@hotmail.com
authors.forename: Ana T
authors.initials: AT
authors.lastname: Tavechio
authors.forename: Angela C R
authors.initials: AC
authors.lastname: Ghilardi
authors.forename: Sueli A
authors.initials: SA
authors.lastname: Fernandes
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D001938:Brazil; D024901:Drug Resistance, Multiple, Bacterial; D005516:Food Microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D019779:Salmonella enterica / Q000145:classification* / Q000187:drug effects; D012486:Salmonella typhimurium / Q000187:drug effects; D012703:Serotyping
chemical_list: D000900:Anti-Bacterial Agents