Detection of hepatitis C virus RNA in formalin-fixed paraffin-embedded sections with digoxigenin-labeled cRNA probes.
Although recent studies have analyzed Hepatitis C (HCV) infections in liver tissue by in situ hybridization (ISH), many of these studies have been of limited diagnostic utility because of the low copy numbers of HCV in formalin-fixed paraffin-embedded (FFPE) tissue and failure to correlate the ISH analysis with other methods of detecting HCV. Thirty six cases of liver biopsies from patients with known HCV antibody status including 20 cases of serum HCV positive and 16 cases of serum HCV negative were analyzed. All cases showed histologic features suggestion of HCV infection. Analyses of all 36 cases were done by RT-PCR combined with Southern hybridization (RT-PCR-SH) and in situ hybridization (ISH). A prolactin riboprobe was used as a negative control. Immunohistochemistry (IHC) with an antibody against HCV (Rb 246) was also used to analyze HCV viral protein in the tissues. Of the 20 serum antibody-positive cases, RT-PCR-SH detected 18 positive cases, while ISH and IHC detected 19 and 16 positive cases, respectively. Of the 16 serum antibody-negative cases, RT-PCR-SH detected 8 positive cases while ISH and IHC detected 8 and 11 positive cases, respectively. A positive ISH signal for HCV was also detected in some lymphocytes and bile ducts in the liver. These results show that ISH with a highly specific riboprobe is comparable to RT-PCR-SH for detection of HCV infection in liver tissue.
Qian
Xiang
X
Department of Laboratory Medicine and Pathology, Mayo Clinic and Mayo Foundation Rochester, MN 55905, USA.
Guerrero
Ruben B
RB
Plummer
Timothy B
TB
Alves
Venancio F
VF
Lloyd
Ricardo V
RV
D001706:Biopsy; D004076:Digoxigenin / Q000737:chemistry; D005404:Fixatives; D005557:Formaldehyde / Q000737:chemistry; D016174:Hepacivirus / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D006508:Hepatitis Antibodies / Q000737:chemistry; D006526:Hepatitis C / Q000175:diagnosis*; D006801:Humans; D007120:Immunochemistry; D017403:In Situ Hybridization / Q000379:methods*; D008099:Liver / Q000473:pathology / Q000821:virology*; D016612:Paraffin Embedding; D011388:Prolactin / Q000737:chemistry; D015347:RNA Probes; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics
D005404:Fixatives; D006508:Hepatitis Antibodies; D015347:RNA Probes; D012367:RNA, Viral; D014764:Viral Proteins; D005557:Formaldehyde; D011388:Prolactin; D004076:Digoxigenin