Increased invasion and expression of MMP-9 in human colorectal cell lines by a CD44-dependent mechanism.
BACKGROUND
In order to investigate the interactions between MMPs and CD44 we stably transfected a non-invasive colon cell line, SW480 with the cDNA for MMP-9 and investigated the effect on CD44 expression and in vitro invasion and migration.


METHODS
In vitro invasion and migration assays were carried out using Biocoat matrigel invasion chambers. MMP and CD44 expression was determined using zymography, Western blot analysis and RT-PCR.


RESULTS
Transfection of the parental SW480 cells with the cDNA for MMP-9 (SW480M9) caused increased invasion and migration. MMP-9 expression increased when the SW480M9 cells were grown on HA and collagen and cultured in the presence of a CD44-activating antibody. Treatment of the cells with HA and a CD44-activating antibody also resulted in increased invasion, migration and attachment.


CONCLUSIONS
These results demonstrated that CD44 and MMP interactions are important in controlling tumour cell invasion and migration.
Murray
David
D
School of Biotechnology, National Institute for Cellular Biotechnology, Dublin City University, Dublin 9, Ireland.
Morrin
Mary
M
McDonnell
Susan
S
D000230:Adenocarcinoma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis* / Q000378:metabolism; D006820:Hyaluronic Acid / Q000378:metabolism / Q000494:pharmacology; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D009361:Neoplasm Invasiveness; D014162:Transfection; D015854:Up-Regulation
D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid; D020780:Matrix Metalloproteinase 9