title: Immunologic response to inhaled endotoxin: changes in peripheral cell surface markers in normal individuals.
abstract: Monocyte cell surface CD14 increases following both in vitro challenge with lipopolysaccharide (LPS) and exposure to organic dusts. We investigated 9 volunteers, mean age 39 years (range, 29-53 years). Each inhaled increasing concentrations of lipopolysaccharide (0.5 microg, 5.0 microg, and 20 microg). Monocyte cell surface CD14 (expressed as mean linear fluorescence) was measured before and after using flow cytometry. Upregulation of CD14 (up to 6 hours after LPS exposure) did not differ significantly between LPS (mean, 35.8; standard deviation [SD]; 54.3), n = 7 after 20 l g LPS) in comparison to placebo (39.3 [49.0]; n = 7). Maximum mean (SD) percentage CD14 upregulation up to 6 hours after challenge differed, but not significantly between those experiencing a clinically significant event (58.4 [49.2]) in comparison to those who did not (13.8, [43.2]; P = 0.27). Two individuals with a marked clinical response developed marked CD14 upregulation after exposure to LPS.
authors.affiliation: Sheffield Occupational and Environmental Lung Injury Centre and Health and Safety Laboratory, Sheffield, UK. david.fishwick@hsl.gov.uk
authors.forename: David
authors.initials: D
authors.lastname: Fishwick
authors.forename: Chris
authors.initials: C
authors.lastname: Barber
authors.forename: Paul
authors.initials: P
authors.lastname: Beckett
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Bradshaw
authors.forename: Roger
authors.initials: R
authors.lastname: Rawbone
authors.forename: Andrew David
authors.initials: AD
authors.lastname: Curran
mesh_terms: D000328:Adult; D005434:Flow Cytometry; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism*; D018950:Lipopolysaccharide Receptors / Q000032:analysis* / Q000378:metabolism; D008875:Middle Aged; D015854:Up-Regulation
chemical_list: D018950:Lipopolysaccharide Receptors