title: [Fusion expression of Neisseria gonorrhoeae outmembrane protein with a mucosal adjuvant].
abstract: OBJECTIVE
  To express a fusion protein of Neisseria gonorrhoeae with a mucosal adjuvant.
  
  
  METHODS
  The gene coding Loop VI-VIII(PL678) of porin, an out-membrane protein of Neisseria gonorrhoeae, was obtained by PCR. It was inserted into a plasmid fused with subunit B of heat labile enterotoxin. The recombinant was transformed in E. coli. The expression of fusion protein was analysed by ELISA, SDS-PAGE and Western-blot.
  
  
  RESULTS
  Fusion protein with LTB was successfully expressed, and displayed both the ability of binding GM1 and the reactogenicity with polyclonal antibodies against Neisseria gonorrhoeae.
  
  
  CONCLUSIONS
  The expression of fusion protein laid a foundation for the study of the intramolecular vaccine against Neisseria gonorrhoeae.
authors.affiliation: College of Medical Laboratory, Third Military Medical University, Chongqing 400038, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Pan
authors.forename: Xuhu
authors.initials: X
authors.lastname: Mao
authors.forename: Weijun
authors.initials: W
authors.lastname: Zhang
authors.forename: Dongshui
authors.initials: D
authors.lastname: Lu
authors.forename: Ping
authors.initials: P
authors.lastname: Luo
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis*; D001427:Bacterial Toxins / Q000096:biosynthesis*; D001428:Bacterial Vaccines / Q000276:immunology; D004768:Enterotoxins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D009344:Neisseria gonorrhoeae / Q000737:chemistry* / Q000276:immunology; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; C027538:heat-labile enterotoxin, E coli