title: Comparative analysis of two 14-3-3 homologues and their expression pattern in the root-knot nematode Meloidogyne incognita.
abstract: 14-3-3 proteins are highly conserved ubiquitous proteins found in all eukaryotic organisms. They are involved in various cellular processes including signal transduction, cell-cycle control, apoptosis, stress response and cytoskeleton organisation. We report here the cloning of two genes encoding 14-3-3 isoforms from the plant parasitic root-knot nematode Meloidogyne incognita, together with an analysis of their expression. Both genes were shown to be transcribed in unhatched second stage larvae, infective second stage larvae, adult males and females. The Mi-14-3-3-a gene was shown to be specifically transcribed in the germinal primordium of infective larvae, whereas Mi-14-3-3-b was transcribed in the dorsal oesophageal gland in larvae of this stage. The MI-14-3-3-B protein was identified by mass spectrometry in in vitro-induced stylet secretions from infective larvae. The stability and distribution of MI-14-3-3 proteins in host plant cells was assessed after stable expression of the corresponding genes in tobacco BY2 cells.
authors.affiliation: INRA. Unité Interactions Plantes-Microorganismes et Santé Végétale. 400, Route des Chappes, BP 167, 06 903 Sophia Antipolis Cedex, France.
authors.forename: S
authors.initials: S
authors.lastname: Jaubert
authors.forename: J-B
authors.initials: JB
authors.lastname: Laffaire
authors.forename: T N
authors.initials: TN
authors.lastname: Ledger
authors.forename: P
authors.initials: P
authors.lastname: Escoubas
authors.forename: E-Z
authors.initials: EZ
authors.lastname: Amri
authors.forename: P
authors.initials: P
authors.lastname: Abad
authors.forename: M N
authors.initials: MN
authors.lastname: Rosso
mesh_terms: D048948:14-3-3 Proteins / Q000032:analysis / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics; D004791:Enzyme Inhibitors / Q000032:analysis; D005260:Female; D017238:Genes, Helminth / Q000235:genetics*; D015801:Helminth Proteins / Q000032:analysis / Q000235:genetics*; D006790:Host-Parasite Interactions / Q000235:genetics; D007814:Larva / Q000235:genetics; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D018613:Microscopy, Confocal / Q000379:methods; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000737:chemistry / Q000235:genetics; D020033:Protein Isoforms / Q000032:analysis / Q000235:genetics; D016415:Sequence Alignment; D014158:Transcription, Genetic / Q000235:genetics; D014431:Tylenchoidea / Q000235:genetics*
chemical_list: D048948:14-3-3 Proteins; D018076:DNA, Complementary; D018509:DNA, Helminth; D004791:Enzyme Inhibitors; D015801:Helminth Proteins; D020033:Protein Isoforms