title: Transcriptional profiling identifies genes differentially expressed during and after migration in murine primordial germ cells.
abstract: Mouse primordial germ cells (PGCs) are migratory until they colonize the genital ridges, assemble with the somatic tissue, and start to differentiate into oocytes or spermatogonia. Using cell transplantation experiments, we show here that germ cells isolated during migration (at E10.5) will migrate actively to the genital ridges, whereas post-migratory PGCs isolated from E12.5 embryos are non-motile even when transferred into a permissive environment (e.g. E10.5 host tissue). Major transcriptional changes must take place between E10.5 and E12.5 that convert germ cells from a migratory to a non-migratory state. To identify the genes involved, we have performed transcriptional profiling of motile and non-motile populations of PGCs. We have identified 55 transcripts that are expressed in E10.5 PGCs at levels at least 3 x their expression at E12.5, and 48 transcripts with the reciprocal expression levels. Additionally, 309 transcripts were found to be expressed in both populations. Many of the E10.5 transcripts encode proteins involved in controlling cytoskeletal and adhesive interactions implicated in cell motility. Many of the E12.5 transcripts encode proteins implicated in germ cell differentiation.
authors.affiliation: Division of Developmental Biology, Children's Hospital Research Foundation, 3333 Burnet Ave., Cincinnati, OH 45229, USA.
authors.forename: Kathleen A
authors.initials: KA
authors.lastname: Molyneaux
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Kyle
authors.initials: K
authors.lastname: Schaible
authors.forename: Chris
authors.initials: C
authors.lastname: Wylie
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology; D005260:Female; D015870:Gene Expression / Q000502:physiology*; D020869:Gene Expression Profiling; D008297:Male; D051379:Mice; D010063:Ovum / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*; D013234:Stem Cells / Q000502:physiology*