Crystallization and preliminary X-ray crystallographic study of the leech protease inhibitor guamerin and its complex with bovine pancreatic chymotrypsin.
Guamerin, a small peptide inhibitor of the serine protease from Hirudo nipponia, was expressed in yeast and crystallized using the vapor diffusion method, with MPD as precipitant. The crystal was found to belong to the monoclinic P2(1) space group with unit cell parameters a=136.06, b=206.59, c=227.39 A, beta=105.03 degrees. The guamerin/bovine pancreatic chymotrypsin complex was also crystallized using PEG 8K as precipitant. The space group was identified as P2(1)2(1)2(1) with unit cell parameters of a=44.01, b=44.30, c=122.47 A. The diffraction data of the complex were collected up to a resolution of 2.4 A using a synchrotron-radiation source under cryogenic condition.
Kim
Dong Ryoung
DR
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, South Korea.
Kim
Dong Young
DY
Chu
Trang Thi Thu
TT
Jung
Kyung-Hwan
KH
Kim
Kyeong Kyu
KK
D000818:Animals; D002417:Cattle; D002918:Chymotrypsin / Q000737:chemistry* / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D007447:Invertebrate Hormones / Q000737:chemistry / Q000378:metabolism*; D007865:Leeches / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology*; D011480:Protease Inhibitors / Q000737:chemistry*
D007447:Invertebrate Hormones; D011480:Protease Inhibitors; C093975:guamerin; D002918:Chymotrypsin