[Establishment of a sandwich ELISA for detecting serum sTRAIL and its application].
OBJECTIVE
To establish quantitative ELISA for detecting souble TRAIL and to evaluate its clinical application.


METHODS
The mAb FMU1.1 against TRAIL was used as coating antibody, and the rabbit polyclonal antibody to TRAIL as a sandwich antibody. The serum sTRAIL levels of nine patients with hemorrhagic fever with renal syndrome and forty psoriasis patients were measured by this method. RESUITS: Sandwich ELISA was established by combination of mAb and pAb. The sensitivity of the ELISA was 0.08 microg/L. The increased levels of serum sTRAIL were found in three cases out of nine HFRS patients and eight cases out of forty psoriasis patients.


CONCLUSIONS
A sandwich ELISA is developed, which provide an useful tool judging the patients condition, curable effect and prognosis of some related diseases.
Li
Ying-Na
YN
Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.
Li
Yi
Y
Liu
Xue-Song
XS
Zhu
Yong
Y
Xu
Xiao-Guang
XG
Jin
Bo-Quan
BQ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D011379:Prognosis; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal