Identification and characterization of a novel mouse prion gene allele.
The major determinant of prion disease incubation time in mice is thought to be the amino acid sequence of the prion protein. Two alleles of the mouse prion gene ( Prnp) have been described, where Prnp(a) (Leu-108, Thr-189) and Prnp(b) (Phe-108, Val-189) are associated with short and long incubation times, to defined prion strains, respectively. As part of a survey of inbred mouse lines, the prion gene open reading frame was sequenced and revealed a new allele, Prnp(c) (Phe-108, Thr-189), in the strain MAI/Pas. To study the influence of Prnp(c) independently of the MAI/Pas genetic background, we generated a congenic line in which Prnp(c) was bred onto the C57BL/6JOlaHsd background. Following intracerebral inoculation with Chandler/RML scrapie prions, the congenic mice showed an increased mean incubation time relative to C57BL/6JOlaHsd, of over 100 days. However, no differences were observed in the intensity and pattern of PrP immunoreactivity deposition or spongiosis. We conclude that the new allele, Prnp(c), modulates incubation time but not neuropathology and that the previous classification of mice into two distinct groups based on incubation time and Prnp genotype should now be revised.
Lloyd
Sarah E
SE
MRC Prion Unit and Department of Neurodegenerative Disease, Institute of Neurology, University College, London, WC1N 3BG, UK.
Thompson
Simon R
SR
Beck
Jonathan A
JA
Linehan
Jacqueline M
JM
Wadsworth
Jonathan D F
JD
Brandner
Sebastian
S
Collinge
John
J
Fisher
Elizabeth M C
EM
D000483:Alleles; D000818:Animals; D003433:Crosses, Genetic; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D011328:Prions / Q000737:chemistry / Q000502:physiology*; D012608:Scrapie / Q000209:etiology*; D013045:Species Specificity; D013329:Structure-Activity Relationship; D013997:Time Factors
D011328:Prions