title: Microsurface plasmon resonance biosensing based on gold-nanoparticle film.
abstract: We use a gold-nanoparticle coated film to achieve highly spatially resolved biosensing that is based on localized surface-plasmon resonance. Unlike the planar gold film employed for conventional surface-plasmon resonance sensing, the gold-nanoparticle film relies exclusively on shifting of the peak extinction wavelength for detection of biointeraction and does not depend critically on the angle of incidence. These characteristics permit integration of surface-plasmon resonance with large-numerical-aperture optics to achieve biosensing with high sensitivity and spatial resolution as high as 25 microm.
authors.affiliation: College of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Hong
authors.forename: Fu-Jen
authors.initials: FJ
authors.lastname: Kao
mesh_terms: D000937:Antigen-Antibody Reactions / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry*; D006046:Gold; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D043942:Nanotubes / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000295:instrumentation / Q000379:methods*; D019809:Streptavidin / Q000032:analysis* / Q000737:chemistry / Q000276:immunology*; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*
chemical_list: D020099:Coated Materials, Biocompatible; D006046:Gold; D019809:Streptavidin