title: Osteoblast responses to tape-cast and sintered bioactive glass ceramics.
abstract: The advantage of tape-cast bioactive glasses lies in the manufacturing procedure, which allows the build-up of layers and, therefore, the production of complex shapes. This, therefore, has applications to tissue engineering, where specific shapes are required such as repair of craniofacial defects. The bioactivity of tape-cast discs sintered at temperatures ranging from 800 degrees C to 1000 degrees C and for 3 or 6 h was analyzed by FTIR. Tape-cast discs were used to culture primary human osteoblasts, and cell attachment, cell death, collagen production, nodule formation, and mineralization were studied. These responses were dependent upon Si and Na release profiles of the tape-cast discs, and development of the hydroxyapatite layer.
authors.affiliation: Department of Materials, Imperial College London, South Kensington Campus, London SW7 2BP, United Kingdom. J.gough@umist.ac.uk
authors.forename: J E
authors.initials: JE
authors.lastname: Gough
authors.forename: D C
authors.initials: DC
authors.lastname: Clupper
authors.forename: L L
authors.initials: LL
authors.lastname: Hench
mesh_terms: D017209:Apoptosis / Q000502:physiology; D001672:Biocompatible Materials; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques; D002516:Ceramics; D003470:Culture Media; D017886:Durapatite / Q000032:analysis / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010006:Osteoblasts / Q000502:physiology*; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D003470:Culture Media; C023506:Glass ceramics; D017886:Durapatite