title: Solasodine glycoside production by hairy root cultures of Physalis minima Linn.
abstract: Hairy root cultures of Physalis minima L. were developed using Agrobacterium rhizogenes, strain ATCC 15834 mediated transformation and grown in half strength of Murashige and Skoog medium containing 8% (w/v) sucrose. Media supplementation with 1 mg naphthalenacetic acid l(-1) and 1 mg benzyladenine increased solasodine glycoside up to 900 g dry wt, which was 20 times higher than that in the native root.
authors.affiliation: Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand. waraporn@kku.ac.th
authors.forename: Waraporn
authors.initials: W
authors.lastname: Putalun
authors.forename: Preeyaporn
authors.initials: P
authors.lastname: Prasamsiwamai
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tanaka
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Shoyama
mesh_terms: D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D032321:Physalis / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology*; D012231:Rhizobium / Q000502:physiology*; D012991:Solanaceous Alkaloids / Q000096:biosynthesis* / Q000302:isolation & purification; D013395:Sucrose / Q000378:metabolism
chemical_list: D010937:Plant Growth Regulators; D012991:Solanaceous Alkaloids; D013395:Sucrose; C026608:alpha-solamargine