Secretory leucoprotease inhibitor impairs Toll-like receptor 2- and 4-mediated responses in monocytic cells.
Secretory leucoprotease inhibitor (SLPI) is an anti-inflammatory antiprotease which can inhibit lipopolysaccharide-induced NF-kappaB activation. We examined its ability to inhibit NF-kappaB activation induced by lipoteichoic acid and investigated the effects of oxidation or complex formation with neutrophil elastase on SLPI's anti-inflammatory properties in U937 myelomonocytic cells and macrophages.
Greene
Catherine M
CM
Respiratory Research Division, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin 9, Ireland. cmgreene@rcsi.ie
McElvaney
Noel G
NG
O'Neill
Shane J
SJ
Taggart
Clifford C
CC
D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009000:Monocytes / Q000276:immunology*; D016328:NF-kappa B / Q000378:metabolism; D010084:Oxidation-Reduction; D010196:Pancreatic Elastase / Q000378:metabolism; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D011956:Receptors, Cell Surface / Q000378:metabolism* / Q000502:physiology*; D053481:Secretory Leukocyte Peptidase Inhibitor; D013682:Teichoic Acids / Q000494:pharmacology; D051195:Toll-Like Receptor 2; D051193:Toll-Like Receptors; D020298:U937 Cells
D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D011956:Receptors, Cell Surface; C506814:SLPI protein, human; D053481:Secretory Leukocyte Peptidase Inhibitor; C495342:TLR2 protein, human; D013682:Teichoic Acids; D051195:Toll-Like Receptor 2; D051193:Toll-Like Receptors; C009900:lipoteichoic acid; D010196:Pancreatic Elastase