title: Contribution of the SgIGSF adhesion molecule to survival of cultured mast cells in vivo.
abstract: Spermatogenic immunoglobulin superfamily (SgIGSF) is a recently identified adhesion molecule, and the microphthalmia transcription factor (MITF) was essential for its expression in mast cells. Since the tg mutant allele is practically a null mutation of the MITF gene, cultured mast cells (CMCs) derived from (WBxC57BL/6)F(1) (F(1))-tg/tg mice did not express SgIGSF whereas CMCs from F(1)-wild-type (+/+) mice expressed it abundantly. When cocultured with NIH/3T3 fibroblasts, F(1)-tg/tg CMCs showed poor adhesion to NIH/3T3 fibroblasts. When injected intraperitoneally, F(1)-tg/tg CMCs showed poor survival in the peritoneal cavity of mast cell-deficient F(1)-W/Wv mice. SgIGSF was expressed in tg/tg CMCs ectopically through retroviral transfection and through expression of a transgene. The resulting tg/tg CMCs showed not only a better adhesion to NIH/3T3 fibroblasts but also a better survival in the peritoneal cavity than control F(1)-tg/tg CMCs. SgIGSF-mediated adhesion seemed to play a role in the survival of CMCs in the peritoneal cavity.
authors.affiliation: Department of Pathology, Osaka University Medical School/Graduate School of Frontier Bioscience, Osaka 565-0871, Japan. akito@med.kobe-u.ac.jp
authors.forename: Akihiko
authors.initials: A
authors.lastname: Ito
authors.forename: Yu-ichiro
authors.initials: Y
authors.lastname: Koma
authors.forename: Kenji
authors.initials: K
authors.lastname: Watabe
authors.forename: Tomoko
authors.initials: T
authors.lastname: Jippo
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Wakayama
authors.forename: Shoichi
authors.initials: S
authors.lastname: Iseki
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Kitamura
mesh_terms: D000483:Alleles; D000818:Animals; D015153:Blotting, Western; D002448:Cell Adhesion; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D002470:Cell Survival; D018920:Coculture Techniques; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D005822:Genetic Vectors; D007136:Immunoglobulins / Q000378:metabolism / Q000502:physiology*; D007150:Immunohistochemistry; D008407:Mast Cells / Q000166:cytology* / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D051739:Microphthalmia-Associated Transcription Factor; D009154:Mutation; D041681:NIH 3T3 Cells; D012190:Retroviridae / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection; D019076:Transgenes
chemical_list: C498598:Cadm1 protein, mouse; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D007136:Immunoglobulins; D008565:Membrane Proteins; D051739:Microphthalmia-Associated Transcription Factor; C497235:Mitf protein, mouse; D014157:Transcription Factors