[Cloning and prokaryotic expression of heat shock protein 70 gene of Mycobacterium tuberculosis].
OBJECTIVE
To clone the heat shock protein 70 (Hsp70) gene of Mycobacterium tuberculosis and express it in E.coli.


METHODS
Hsp70 gene was amplified by PCR from Mycobacterium tuberculosis H37Rv, then inserted into pUC19 vector and sequenced. The recombinant expression plasmid pGEX-TBhsp70 was constructed and expressed in E.coli.


RESULTS
Hsp70 gene was obtained form Mycobacterium tuberculosis and its sequence was identical with that reported in GenBank. The E.coli. containing pGEX-TBhsp70 could express a protein with M(r) being 96,000 after induction of IPTG.


CONCLUSIONS
A confirmed Hsp70 gene is cloned and expressed in E.coli successfully, which lay the foundation for the related research of the gene.
Ye
Jing
J
Department of Pathology, Fourth Military Medical University, Xi'an 710032, China.
Sui
Yan-fang
YF
Chen
Guang-sheng
GS
Zhang
Xiu-min
XM
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D010957:Plasmids
D018840:HSP70 Heat-Shock Proteins