title: Differential change in mRNA expression of p75 and Trk neurotrophin receptors in nucleus gracilis after spinal nerve ligation in the rat.
abstract: In peripheral neuropathy (PN), dorsal column (DC) fibers that synapse in the nucleus gracilis (NuGr) mediate expression of mechanical allodynia and have increased expression of brain-derived neurotrophic factor (BDNF). Neurotrophins (NTs) are implicated in pathology or repair in PN. To assess NTs in the NuGr in PN, mRNA expression of BDNF, nerve growth factor (NGF), and NT receptors TrkA, TrkB, and p75 was determined 1 week after ligation of L5 and L6 spinal nerves (SNL). Laser capture microdissection was used to collect NuGr tissue followed by reverse-transcription (RT)-PCR. TrkA, TrkB, and NGF mRNA levels decreased, whereas p75 mRNA increased, in ipsilateral SNL NuGr compared with SHAM; BDNF was undetectable. Decreased Trk mRNA may result in decreased NT activity in the NuGr. The p75 receptor influences Trk activity and cell survival, thus its role in PN warrants further investigation.
authors.affiliation: Division of Rheumatology, Immunology and Allergy, Department of Internal Medicine, S-2056 William H. Davis Medical Research Center, The Ohio State University College of Medicine and Public Health, Columbus, OH 43210, USA. goettl.l@osu.edu
authors.forename: Virginia M
authors.initials: VM
authors.lastname: Goettl
authors.forename: Syed-Rehan A
authors.initials: SR
authors.lastname: Hussain
authors.forename: Oscar
authors.initials: O
authors.lastname: Alzate
authors.forename: Dylan J
authors.initials: DJ
authors.lastname: Wirtz
authors.forename: Robert L
authors.initials: RL
authors.lastname: Stephens
authors.forename: Kevin V
authors.initials: KV
authors.lastname: Hackshaw
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics; D004195:Disease Models, Animal; D018450:Disease Progression; D005786:Gene Expression Regulation; D008026:Ligation; D008297:Male; D008526:Medulla Oblongata / Q000378:metabolism* / Q000473:pathology; D020932:Nerve Growth Factor / Q000235:genetics; D010523:Peripheral Nervous System Diseases / Q000473:pathology / Q000503:physiopathology*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020800:Receptor, Nerve Growth Factor; D020917:Receptor, trkA / Q000235:genetics*; D020813:Receptor, trkB / Q000235:genetics*; D017475:Receptors, Nerve Growth Factor / Q000235:genetics*; D013127:Spinal Nerves / Q000293:injuries / Q000378:metabolism / Q000473:pathology
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor; D020932:Nerve Growth Factor; D020917:Receptor, trkA; D020813:Receptor, trkB