Rapid enumeration of virus-like particles in drinking water samples using SYBR green I-staining.
We studied the suitability of SYBR green I-staining for determining total counts of virus-like particles and bacteria in drinking water. Low background fluorescence and lack of unspecific staining made drinking water samples an excellent matrix for SYBR green I-staining. Direct microscopic count method is a rapid and economical tool for assessing the total number of virus-like particles in aquatic samples, compared to culture-dependent or molecular biology methods. We applied this method to show the efficiency of a large-scale drinking water purification process in the removal of virus-like particles and bacteria from lake water.
Rinta-Kanto
Johanna M
JM
Department of Environmental Health, National Public Health Institute, P.O. Box 95, FIN-70701 Kuopio, Finland.
Lehtola
Markku J
MJ
Vartiainen
Terttu
T
Martikainen
Pertti J
PJ
D052160:Benzothiazoles; D015169:Colony Count, Microbial; D003959:Diamines; D005374:Filtration; D005618:Fresh Water / Q000821:virology*; D008856:Microscopy, Fluorescence; D009930:Organic Chemicals; D011804:Quinolines; D012621:Seasons; D013194:Staining and Labeling / Q000379:methods*; D019562:Viral Load; D014780:Viruses / Q000302:isolation & purification; D014871:Water Microbiology; D018508:Water Purification; D014881:Water Supply
D052160:Benzothiazoles; D003959:Diamines; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I