title: Selective adsorption of human CD4 T cells.
abstract: The pathogenesis of most autoimmune diseases directly involves CD4(+) helper T cells. To remove CD4(+) T cells selectively from the circulation, we designed a new column in which an anti-CD4 monoclonal antibody was immobilized on the activated substance. Nearly 90% of CD4(+) T cells were selectively adsorbed from whole blood with a single passage through the column in vitro, resulting in depletion of the antigen-specific T cell responses. We conclude that this new column would be potentially useful for treatment of T cell-mediated autoimmune diseases.
authors.affiliation: Division of Clinical Research, Kawatana National Hospital, Nagasaki, Japan. hidenori@kawatana.hosp.go.jp
authors.forename: Hidenori
authors.initials: H
authors.lastname: Matsuo
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Goto
authors.forename: Chiaki
authors.initials: C
authors.lastname: Kambara
authors.forename: Takayasu
authors.initials: T
authors.lastname: Fukudome
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Mizota
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Onodera
authors.forename: Makoto
authors.initials: M
authors.lastname: Yoshida
authors.forename: Noritoshi
authors.initials: N
authors.lastname: Shibuya
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002469:Cell Separation / Q000379:methods*; D016238:Cytapheresis / Q000379:methods*; D005434:Flow Cytometry; D006801:Humans
chemical_list: D000911:Antibodies, Monoclonal; D015704:CD4 Antigens