A survey of the prevalence of infectious bronchitis virus type 4/91 in Iran.
To evaluate the prevalence of infectious bronchitis virus (IBV) type 4/91 in Iran, tracheal swabs from 77 broiler flocks in 16 provinces were collected at the slaughterhouse. Swabs were subjected to RNA extraction and tested by RT-PCR, followed by a type-specific nested PCR. The viral RNA was detected in 33 samples (42.8%) from different provinces. The results indicate a relatively high prevalence of IBV type 4/91 in Iran and necessitate revising the vaccination programme against this disease.
Seyfi Abad Shapouri
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MR
Section of Virology, Veterinary School of Shahid Chamran University of Ahwaz, Iran. masoudrs@yahoo.com
Mayahi
M
M
Assasi
K
K
Charkhkar
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S
D000818:Animals; D002645:Chickens; D018352:Coronavirus Infections / Q000453:epidemiology / Q000662:veterinary*; D017931:DNA Primers; D001351:Infectious bronchitis virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D007492:Iran / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011201:Poultry Diseases / Q000453:epidemiology* / Q000821:virology; D015995:Prevalence; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
D017931:DNA Primers; D012367:RNA, Viral