title: Effects of customized bicarbonate buffered solutions for continuous renal replacement therapies on polymorphonuclear leukocytes function and viability.
abstract: It has previously been shown that the mixture of bicarbonate and calcium in the solutions used for continuous renal replacement therapy led to crystallization and significant changes in calcium concentration and pH. The aim of this study was to investigate the impact of bicarbonate/calcium and lactate/calcium solutions for Continuous Renal Replacement Therapies (CRRT) on the viability and function of polymorphonuclear cells (PMN). We tested four customized bicarbonate buffered solutions: single bag (bicarbonate and calcium mixed 24 h before testing), double bag (mixed immediately before testing), filtered single bag and double bag solutions, and a commercial lactate buffered solution. Blood from 6 volunteers was incubated with the solutions for 30 min followed by PMN isolation. After overnight incubation, viability, phagocytosis, and peroxide production by PMN were determined by flow cytometry. There was no difference between the test solutions with respect to PMN viability and function. Therefore, the presence of microcrystals and the consequent changes in electrolyte concentrations do not seem to impair PMN function.
authors.affiliation: Division of Nephrology, Universidade Federal de São Paulo-UNIFESP, São Paulo, Brazil. emaccariello@nephro.com.br
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Maccariello
authors.forename: Maria A
authors.initials: MA
authors.lastname: Dalboni
authors.forename: Sérgio A
authors.initials: SA
authors.lastname: Draibe
authors.forename: Eduardo
authors.initials: E
authors.lastname: Rocha
authors.forename: Oscar Fernando
authors.initials: OF
authors.lastname: Pavão dos Santos
authors.forename: Bento
authors.initials: B
authors.lastname: Santos
authors.forename: Miguel
authors.initials: M
authors.lastname: Cendoroglo
mesh_terms: D000704:Analysis of Variance; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D001639:Bicarbonates / Q000494:pharmacology*; D002021:Buffers; D002470:Cell Survival / Q000187:drug effects; D005069:Evaluation Studies as Topic; D015312:Hemodialysis Solutions / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008422:Materials Testing; D009336:Necrosis; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology*; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology; D011336:Probability; D006435:Renal Dialysis / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D001639:Bicarbonates; D002021:Buffers; D015312:Hemodialysis Solutions