title: The in vitro antibacterial/synergistic activities of Withania somnifera extracts.
abstract: The methanol, hexane and diethyl ether extracts from both leaves and roots of Withania somnifera were evaluated for the antibacterial/synergistic activity by agar plate disc-diffusion assay against Salmonella typhimurium and Escherichia coli. Different concentrations of Tibrim, a combination of rifampicin and isoniazid, were tested to find out the minimum inhibitory concentration (MIC), which came out to be 0.1 mg/ml for S. typhimurium and E. coli. From the six extracts tested, only methanol and hexane extracts of both leaves and roots were found to have potent antibacterial activity. A synergistic increase in the antibacterial effect of Tibrim was noticed when MIC of Tibrim was supplemented with these extracts.
authors.affiliation: Department of Botanical Sciences, Guru Nanak Dev University, Amritsar 143 005, India. gndu.botanical@vsnl.com
authors.forename: S
authors.initials: S
authors.lastname: Arora
authors.forename: S
authors.initials: S
authors.lastname: Dhillon
authors.forename: G
authors.initials: G
authors.lastname: Rani
authors.forename: A
authors.initials: A
authors.lastname: Nagpal
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D004357:Drug Synergism; D004926:Escherichia coli / Q000187:drug effects*; D006801:Humans; D007538:Isoniazid / Q000008:administration & dosage / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves; D018517:Plant Roots; D012293:Rifampin / Q000008:administration & dosage / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000187:drug effects*; D032341:Withania
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D007538:Isoniazid; D012293:Rifampin