title: [Detection of C5a receptor expressions on human epiretinal membranes and cultured RPE cells by immunohistochemical staining].
abstract: OBJECTIVE
  To observe the expression and distribution of C5a receptor(C5aR) on epiretinal membranes (ERM) tissue from patients with proliferative retreous body-retina (PVR) lesion and cultured retinal pigmentary epithelial (RPE) cells.
  
  
  METHODS
  The C5aR expressions on ERM tissue and cultured RPE cells were detected by ABC immunohistochemical staining with mouse anti-human C5aR mcAbs.
  
  
  RESULTS
  Expression of C5aR was found in ERM tissue and on cultured RPE cells as well as macrophages.
  
  
  CONCLUSIONS
  As a pro-inflammatory factor, binding of C5a to C5aR on RPE cells may play an important role in the development of PVR.
authors.affiliation: Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Wang
authors.forename: Yan-Nian
authors.initials: YN
authors.lastname: Hui
authors.forename: Yu-Sheng
authors.initials: YS
authors.lastname: Wang
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Wang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015936:Complement C5a; D019773:Epiretinal Membrane; D015870:Gene Expression; D006801:Humans; D008264:Macrophages / Q000378:metabolism; D044087:Receptor, Anaphylatoxin C5a; D013194:Staining and Labeling
chemical_list: D044087:Receptor, Anaphylatoxin C5a; D015936:Complement C5a