Growth of Piscirickettsia salmonis to high titers in insect tissue culture cells.
Piscirickettsia salmonis was grown in established insect, frog, and fish tissue culture cells. The yield of P. salmonis in Sf21 cells was up to 100 times that obtained in CHSE-214 cells, and virulence for Atlantic salmon was retained. The ceiling temperature for growth of P. salmonis in Sf21 cells was 24 degrees C.
Birkbeck
T Harry
TH
Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom. T.H.Birkbeck@bio.gla.ac.uk
Griffen
Angela A
AA
Reid
Helen I
HI
Laidler
L Anthony
LA
Wadsworth
Simon
S
D000818:Animals; D001431:Bacteriological Techniques; D002478:Cells, Cultured; D005393:Fish Diseases / Q000382:microbiology / Q000401:mortality; D044147:Piscirickettsiaceae / Q000254:growth & development* / Q000472:pathogenicity; D044225:Piscirickettsiaceae Infections / Q000382:microbiology / Q000662:veterinary; D020317:Salmo salar / Q000382:microbiology*; D018411:Spodoptera / Q000382:microbiology*; D014774:Virulence