Changes in whole cell-derived fatty acids induced by naphthalene in bacteria from genus Pseudomonas.
Fatty acid composition during naphthalene utilization was investigated in three strains of bacteria Pseudomonas vesicularis, Pseudomonas stutzeri and Pseudomonas sp. JS150 that expressed different naphthalene degradation abilities. All strains significantly changed their cellular fatty acid profiles as a response to naphthalene exposure. Since naphthalene was present in the medium P. stutzeri increased ratio of saturated/unsaturated fatty acids from 1.1 to 2.1 and Pseudomonas sp. JS150 from 7.5 to 12.0, respectively. In contrast, this ratio decreased from 2.1 to 1.1 in P. vesicularis under the same growth conditions. The changes comprised also alterations in the percentage of selected groups of fatty acids: iso and anteiso, hydroxy and cyclopropane fatty acids. Our results showed that naphthalene induced in tested strains different changes in fatty acids composition. It may suggest that in the presence of naphthalene microorganisms used different adaptive mechanisms to maintain the cells in appropriate physiological state.
Mrozik
Agnieszka
A
Department of Biochemistry, University of Silesia, Jagielloánska 28, 40-032 Katowice, Poland. amrozik@us.edu.pl
Piotrowska-Seget
Zofia
Z
Łabuzek
Sylwia
S
D001673:Biodegradation, Environmental; D018533:Biomass; D002849:Chromatography, Gas; D003521:Cyclopropanes / Q000032:analysis / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis* / Q000096:biosynthesis / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000737:chemistry; D007700:Kinetics; D009281:Naphthalenes / Q000378:metabolism*; D011549:Pseudomonas / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism*
D003521:Cyclopropanes; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D009281:Naphthalenes; C028775:cyclopropane fatty acids; C031721:naphthalene