A novel ALL-L3 cell line, BALM-25, expressing both immunoglobulin light chains.
A human acute lymphoblastic leukemia (ALL)-derived cell line, BALM-25, was established from the bone marrow specimen of a 59-year-old male patient with B-cell ALL L3 type (ALL-L3) at diagnosis. Immunophenotyping indicated mature B-cell characteristics including expression of cell surface and cytoplasmic immunoglobulin (Ig) chains, CD10, CD19, CD20, CD38, CD39, CD40, CD71, NU-B1 and HLA class II. T-cell and myeloid associated antigens tested were negative except CD5. BALM-25 cells have a morphological appearance typical for L3-type lymphoblasts. Regarding the expression of Ig chains, while the original leukemia cells expressed Ig lambda delta mu and hence a single light (L) chain isotype, the established line revealed double L chain expression both at the cell surface and the cytoplasmic level. Definitive double L chain expression was confirmed by flow cytometry and Western blot analysis. Southern blot analysis demonstrated rearrangement of the IgJH, the Ckappa and the Clambda genes. Cytogenetic analysis of BALM-25 revealed the following numerical and structural abnormalities: 55, X, add(X)(q12), + 2, add(3)(p21), + 5, add(7)(p13), add(11)(p11.2), add(11)(q?23), add(12)(p11.2), add(14)(q22), - 15, + 16, + 16,add(18)(11.2), + 20, + marl, + mar2, + mar3, + mar,inc. The established cell line, BALM-25, provides an unlimited supply of cell material for analyzing the unique (patho)physiology of Ig expression in general and for clarifying the pathogenesis of this type of B-cell malignancy in particular.
Matsuo
Yoshinobu
Y
Fujisaki Cell Center, Hayashibara Biochemical Labs. Inc., Okayama 702-8006, Japan. yomatsuo@hayashibara.co.jp
Drexler
Hans G
HG
Harashima
Akira
A
Takenaka
Katsuto
K
Ishihara
Toshimichi
T
Ishimaru
Fumihiko
F
Orita
Kunzo
K
D015703:Antigens, CD / Q000032:analysis; D015139:Blotting, Southern; D015153:Blotting, Western; D002051:Burkitt Lymphoma / Q000209:etiology / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D020031:Epstein-Barr Virus Infections; D015321:Gene Rearrangement; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D016130:Immunophenotyping; D008297:Male; D008875:Middle Aged
D015703:Antigens, CD; D007147:Immunoglobulin Light Chains