Combining different 'omics' technologies to map and validate protein-protein interactions in humans.
The mapping of protein-protein interactions is key to understanding biological processes. Many technologies have been reported to map interactions and these have been systematically applied in yeast. To date, the number of reported yeast protein interactions that have been truly validated by at least one other approach is low. The mapping of human protein interaction networks is even more complicated. Thus, it is unreasonable to try to map the human interactome; instead, interaction mapping in human cell lines should be focused along the lines of diseases or changes that can be associated with specific cells. In this paper, an approach for combining different 'omics' technologies to achieve efficient mapping and validation of protein interactions in human cell lines is presented.
Figeys
Daniel
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MDS-Proteomics, 251 Attwell Drive, Toronto, Ontario M9W 7H4, Canada. dfigeys@mdsp.com
D002460:Cell Line; D002845:Chromatography / Q000379:methods; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins / Q000378:metabolism; D011485:Protein Binding; D040901:Proteomics / Q000379:methods*; D020798:Two-Hybrid System Techniques
D018076:DNA, Complementary; D005656:Fungal Proteins; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins