Gelatinolytic and fibrinolytic activity in fresh-frozen plasma.
Fresh-frozen plasma (FFP) was evaluated for gelatinolytic and fibrinolytic activity. Gelatin zymography revealed that gelatinase A (MMP-2) was constitutively present in FFP whereas gelatinase B (MMP-9) was present at variable levels. The presence of MMP-9 likely represents differential release from neutrophils during FFP collection or processing. Although fibrin matrices generated from FFP or freshly prepared plasma contained characteristic crosslinked gamma-gamma dimers and beta-monomers, matrices generated from FFP were resistant to spontaneous plasmin-dependent fibrinolysis. This observation likely stems from the plasminogen activator instability and could potentially lead to a hypofibrinolytic state. The impact of these in vitro findings to protease balance in patients receiving multiple FFP doses remains to be determined.
Makowski
Gregory S
GS
Department of Laboratory Medicine, School of Medicine, University of Connecticut Health Center, Farmington, CT 06030-2235, USA. makowski@nso1.uchc.edu
Ramsby
Melinda L
ML
D019281:Dimerization; D005337:Fibrin / Q000378:metabolism; D005342:Fibrinolysis; D018093:Gelatinases / Q000097:blood / Q000378:metabolism*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D010949:Plasma / Q000201:enzymology* / Q000378:metabolism*
D005337:Fibrin; D018093:Gelatinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9