title: Effects of fibrin sealant protein concentrate with and without platelet-released growth factors on bony healing of cortical mandibular defects. An experimental study in minipigs.
abstract: In this study, the effects of fibrin sealant protein concentrate (FSPC; from the Tissucol Kit, Baxter, Vienna, Austria) with and without platelet-released growth factors (PRGF) on bony healing of cortical mandibular defects were investigated. Defects made in the facial mandibular wall of eight adult minipigs with a hollow drill were filled with FSPC alone, a mix of FSPC+PRGF or left untreated as controls. The animals were killed 4 and 8 weeks later. Specimens were processed for histology with the Donath method (1988) and stained with the Levai-Laczko stain. On histology, periosteal osteoneogenesis exceeded endosteal bone formation. No difference was seen in bone formation between FSPC alone, FSPC+PRGF and the controls at 4 and 8 weeks. On histomorphometry, there was no significant difference between the three groups in terms of the percent of newly formed bone at 4 and 8 weeks (P=0.6977). This study showed that FSPC neither increased nor decreased the amount of newly formed bone vs. controls and that the addition of PRGF had no effects on bone regeneration of cortical minipig bone at 4 and 8 weeks.
authors.affiliation: Department of Oral Surgery, University of Vienna Dental School, Vienna, Austria. gabor.fuerst@univie.ac.at
authors.forename: Gabor
authors.initials: G
authors.lastname: Fuerst
authors.forename: Reinhard
authors.initials: R
authors.lastname: Gruber
authors.forename: Stefan
authors.initials: S
authors.lastname: Tangl
authors.forename: Fidel
authors.initials: F
authors.lastname: Sanroman
authors.forename: Georg
authors.initials: G
authors.lastname: Watzek
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects; D005260:Female; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use*; D006490:Hemostatics / Q000627:therapeutic use*; D007091:Image Processing, Computer-Assisted; D016018:Least-Squares Analysis; D008297:Male; D008334:Mandible / Q000187:drug effects / Q000473:pathology; D008336:Mandibular Diseases / Q000188:drug therapy* / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects; D010521:Periosteum / Q000187:drug effects / Q000473:pathology; D010982:Platelet-Derived Growth Factor / Q000627:therapeutic use*; D013552:Swine; D013556:Swine, Miniature; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects
chemical_list: D015718:Fibrin Tissue Adhesive; D006490:Hemostatics; D010982:Platelet-Derived Growth Factor