title: Arthrobacter nitroguajacolicus sp. nov., a novel 4-nitroguaiacol-degrading actinobacterium.
abstract: Three bacterial isolates from soil, capable of degradation or transformation of nitroaromatic compounds and displaying a rod-coccus growth cycle, were studied by a polyphasic approach. On the basis of 16S rRNA sequence analysis and of chemotaxonomic characteristics, such as type A3alpha peptidoglycan with an interpeptide bridge Ala-Thr-Ala, the major menaquinone MK-9(H(2)) and fatty acid composition, the isolates were assigned to the genus Arthrobacter. DNA-DNA hybridization, riboprinting and phenotypic studies revealed that the three strains constitute a single species, distinct from phylogenetically neighbouring Arthrobacter aurescens and Arthrobacter ilicis. A novel species, Arthrobacter nitroguajacolicus sp. nov., with the type strain G2-1(T) (=CCM 4924(T)=DSM 15232(T)) is proposed.
authors.affiliation: Department of Microbiology, Faculty of Science, Masaryk University Brno, Tvrdého 14, 602 00 Brno, Czech Republic.
authors.forename: Ludmila
authors.initials: L
authors.lastname: Kotoučková
authors.affiliation: DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.affiliation: Regional Institute of Public Health, Ostrava, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Durnová
authors.affiliation: DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
authors.forename: Cathrin
authors.initials: C
authors.lastname: Spröer
authors.affiliation: CCM - Czech Collection of Microorganisms, Masaryk University Brno, Czech Republic.
authors.forename: Ivo
authors.initials: I
authors.lastname: Sedláček
authors.affiliation: Veterinary Research Institute, Brno, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Neča
authors.affiliation: Laboratory of Functional Genomics and Proteomics, Faculty of Science, Masaryk University Brno, Czech Republic.|Institute of Analytical Chemistry, Brno, Czech Republic.
authors.forename: Zbyněk
authors.initials: Z
authors.lastname: Zdráhal
authors.affiliation: Department of Microbiology, Faculty of Science, Masaryk University Brno, Tvrdého 14, 602 00 Brno, Czech Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Němec
mesh_terms: D000595:Amino Acid Sequence; D001173:Arthrobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001482:Base Composition; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006139:Guaiacol / Q000031:analogs & derivatives / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000235:genetics; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D006139:Guaiacol