title: Semen characteristics, cryopreservation, and successful artificial insemination in the Blue rock pigeon (Columba livia).
abstract: The present study was undertaken in the Blue rock pigeon (Columba livia) to evaluate the annual semen characteristics, to identify a suitable extender for semen short-term storage, to determine a protocol for cryopreservation of semen and finally to check whether intracloacal insemination would lead to the birth of a chick. Semen characteristics such as semen volume, sperm concentration, sperm motility, and percentage of normal spermatozoa were maximum during the monsoon season. TALP was observed to be the most suitable semen extender and the sperm survived best at 37 degrees C at a dilution of 1:100 in TALP. Further, cryopreservation studies on pigeon semen indicated that 8% DMSO with or without egg yolk (20%) proved to be a better cryoprotectant compared to glycerol and polyethylene glycol. In addition, the slow freezing protocol was better than the fast-freezing protocol and about 40% of the cryopreserved spermatozoa were motile following thawing. Computer-aided semen analysis indicated that pigeon spermatozoa were extremely active immediately after dilution in TALP and exhibited linear trajectories persisting up to 9h. But, with time there was a time-dependent decrease in the velocity parameters (VAP, VSL, and VCL). Cryopreserved spermatozoa following thawing also exhibited linear trajectories but had reduced velocity as evident from the significant decrease in VAP, VSL, and VCL. Further, artificial inseminations using fresh semen resulted in 45% fertilization and birth of a live chick.
authors.affiliation: Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
authors.forename: Sadanand D
authors.initials: SD
authors.lastname: Sontakke
authors.forename: Govindaswamy
authors.initials: G
authors.lastname: Umapathy
authors.forename: Vedantam
authors.initials: V
authors.lastname: Sivaram
authors.forename: Sanjiva D
authors.initials: SD
authors.lastname: Kholkute
authors.forename: Sisinthy
authors.initials: S
authors.lastname: Shivaji
mesh_terms: D000818:Animals; D010856:Columbidae / Q000502:physiology*; D015925:Cryopreservation / Q000662:veterinary*; D005260:Female; D007315:Insemination, Artificial / Q000662:veterinary*; D007700:Kinetics; D008297:Male; D012621:Seasons; D012661:Semen / Q000502:physiology*; D012662:Semen Preservation / Q000662:veterinary*; D013081:Sperm Motility