[Construction of VEGF eukaryotic expression vector and its expression in endothelial cells and cardiac myocytes in-vitro].
OBJECTIVE
To study the feasibility of VEGF165 gene transfection to endothelial cells and cardiac myocytes in-vitro.


METHODS
The VEGF165 DNA fragment was inserted into a green fluorescent protein fusion vector, which was then transfected into endothelial cells and cardiac myocytes with lipofectamine.


RESULTS
The endothelial cells and cardiac myocytes were successfully transfected with pIRES2-EGFP-hVEGF165 gene, which was confirmed by fluoroscopy and immunohistochemistry.


CONCLUSIONS
VEGF can be expressed stably in the endothelial cells and cardiac myocytes, which might be useful for the gene therapy of myocardial ischemia.
Yu
Ming
M
Department of Ultrasound, Xijing Hospital, Fourth Military Medical University, Xi'an 710032,China.
Qian
Yun-Qiu
YQ
Lu
Shao-Ying
SY
Chen
Guang-Sheng
GS
Ye
Jing
J
D042783:Endothelial Cells / Q000378:metabolism; D056890:Eukaryota / Q000235:genetics; D032383:Myocytes, Cardiac / Q000378:metabolism; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
D042461:Vascular Endothelial Growth Factor A