Streptococcal antigen I/II binds to extracellular proteins through intermolecular beta-sheets.
One of the functions associated with the oral streptococcal surface protein I/II is to bind to human extracellular matrix molecules or blood components, which could act as opportunistic ligands in pathological circumstances. In order to understand the relative specificity of the binding repertoire of this bacterial adhesin, we examined by infrared measurements the mode of binding of the protein I/II from Streptococcus mutans OMZ175 (I/IIf) to fibronectin and fibrinogen. This approach revealed the beta-structure forming capacity of I/IIf upon interaction with both proteins. The forming of intermolecular beta-structures may provide a non-selective way of interaction between I/IIf and its possible targets.
Kelemen
Lóránd
L
Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt 62, P.O.B. 521, H-6701 Szeged, Hungary.
Rizk
Samer
S
Debreczeny
Mónika
M
Ogier
Joelle
J
Szalontai
Balázs
B
D018829:Adhesins, Bacterial / Q000378:metabolism; D000577:Amides / Q000737:chemistry; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005340:Fibrinogen / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D008562:Membrane Glycoproteins; D011485:Protein Binding; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013295:Streptococcus mutans / Q000378:metabolism*
D018829:Adhesins, Bacterial; D000577:Amides; D001426:Bacterial Proteins; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; C055764:S-layer proteins; D005340:Fibrinogen