Determination of (+)-3,3',5-triiodo-L-thyronine (L-T3) from serum using a sequential injection analysis/immunosensor system.
A sequential injection analysis/immunosensor system is proposed for the analysis of T3 in serum with a rate of 75 samples/hr. The immunosensor design is based on the physical immobilization of anti-T3 in carbon paste. The working concentration range of the immunosensor in a sequential injection analysis system is between 3.4 and 340 ng/mL with a limit of detection of 2.19 ng/mL. The system is very reliable and very easy to design and operate.
Stefan
Raluca-Ioana
RI
Department of Chemistry, University of Pretoria, Pretoria, South Africa. raluca.stefan@chem.up.ac.za
van Staden
Jacobus Frederick
JF
Aboul-Enein
Hassan Y
HY
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014284:Triiodothyronine / Q000097:blood*
D014284:Triiodothyronine