title: Some lung cellular parameters reflecting inflammation after combined inhalation of amosite dust with cigarette smoke by rats.
abstract: Cellular changes were followed in lung cell suspensions after 175 day inhalation by rats of concentrations 30 mg/m3 or 60 mg/m3 of amosite asbestos every second day combined with daily exposure to cigarette smoke at 30 mg of total particulate matter (TPM)/m3 air. Concomitantly, lung inflammation was assessed by changes in the bronchoalveolar lavage fluid (BALF). A dose-dependent rise in the BALF inflammatory parameters was found. The rise of the proportion of binucleate (BNC) and multinucleate cells (MNC) in lung cell suspensions was also dose-dependent. It is concluded that, in the experimental assessment of effects of fibrogenic dusts, the number of BNC and of MNC in lung cell suspensions may serve as a useful semiquantitative biomarker of the inflammation.
authors.affiliation: Slovak Medical University, Institute of Preventive and Clinical Medicine, Bratislava, Slovak Republic. mbeno@upkm.sk
authors.forename: M
authors.initials: M
authors.lastname: Beno
authors.forename: M
authors.initials: M
authors.lastname: Hurbánková
authors.forename: M
authors.initials: M
authors.lastname: Dusinská
authors.forename: K
authors.initials: K
authors.lastname: Volkovová
authors.forename: M
authors.initials: M
authors.lastname: Staruchová
authors.forename: S
authors.initials: S
authors.lastname: Cerná
authors.forename: M
authors.initials: M
authors.lastname: Barancoková
authors.forename: A
authors.initials: A
authors.lastname: Kazimirová
authors.forename: Z
authors.initials: Z
authors.lastname: Kováciková
authors.forename: P
authors.initials: P
authors.lastname: Bobek
authors.forename: M
authors.initials: M
authors.lastname: Horecký
authors.forename: M
authors.initials: M
authors.lastname: Mikulecký
authors.forename: S A
authors.initials: SA
authors.lastname: Kyrtopoulos
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D017639:Asbestos, Amosite / Q000633:toxicity*; D001992:Bronchoalveolar Lavage Fluid; D002452:Cell Count; D004305:Dose-Response Relationship, Drug; D004391:Dust; D007249:Inflammation; D008168:Lung / Q000166:cytology / Q000473:pathology*; D008264:Macrophages / Q000187:drug effects; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D012907:Smoking / Q000009:adverse effects*
chemical_list: D004391:Dust; D017639:Asbestos, Amosite