A novel rice PR10 protein, RSOsPR10, specifically induced in roots by biotic and abiotic stresses, possibly via the jasmonic acid signaling pathway.
Plant roots have important roles not only in absorption of water and nutrients, but also in stress tolerance such as desiccation, salt, and low temperature. We have investigated stress-response proteins from rice roots using 2-dimensional polyacrylamide-gel electrophoresis and found a rice protein, RO-292, which was induced specifically in roots when 2-week-old rice seedlings were subjected to salt and drought stress. The full-length RO-292 cDNA was cloned, and was determined to encode a protein of 160 amino acid residues (16.9 kDa, pI 4.74). The deduced amino acid sequence showed high similarity to known rice PR10 proteins, OsPR10a/PBZ1 and OsPR10b. RO-292 mRNA accumulated rapidly upon drought, NaCl, jasmonic acid and probenazole, but not by exposure to low temperature or by abscisic acid and salicylic acid. The RO-292 gene was also up-regulated by infection with rice blast fungus. Interestingly, induction was observed almost exclusively in roots, thus we named the gene RSOsPR10 (root specific rice PR10). The present results indicate that RSOsPR10 is a novel rice PR10 protein, which is rapidly induced in roots by salt, drought stresses and blast fungus infection possibly through activation of the jasmonic acid signaling pathway, but not the abscisic acid and salicylic acid signaling pathway.
Hashimoto
Makoto
M
Department of Biological Sciences, Tokyo Metropolitan University, Minami-Osawa, Hachioji-shi, Tokyo, 192-0397 Japan.
Kisseleva
Larisa
L
Sawa
Shinichiro
S
Furukawa
Toshiko
T
Komatsu
Setsuko
S
Koshiba
Tomokazu
T
D000040:Abscisic Acid / Q000378:metabolism / Q000494:pharmacology; D003517:Cyclopentanes / Q000378:metabolism*; D018076:DNA, Complementary / Q000032:analysis; D004190:Disasters; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D008969:Molecular Sequence Data; D009181:Mycoses / Q000235:genetics; D012275:Oryza / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D054883:Oxylipins; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D018517:Plant Roots / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D015398:Signal Transduction / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology
D003517:Cyclopentanes; D018076:DNA, Complementary; D054883:Oxylipins; D010940:Plant Proteins; D012333:RNA, Messenger; D013844:Thiazoles; C099063:oryzemate; D012965:Sodium Chloride; C011006:jasmonic acid; D000040:Abscisic Acid; D020156:Salicylic Acid