title: Clinostomum complanatum and Clinostomum marginatum (Rudolphi, 1819) (Digenea: Clinostomidae) are separate species based on differences in ribosomal DNA.
abstract: Infections by metacercariae of Clinostomum (Leidy, 1856) species adversely affect aquacultured fish and are potentially transmissible to humans. Molecular methodologies are efficient tools, which enable diagnosis of all life-history stages of trematodes in their diverse hosts. The small subunit of ribosomal DNA genes of adults of the Old World Clinostomum complanatum (Rudolphi, 1819) and the New World Clinostomum marginatum (Rudolphi, 1819), obtained from a little egret Egretta garzetta (Linnaeus, 1766) and the great blue heron Ardea herodias (Linnaeus, 1758), respectively, were amplified, sequenced, and aligned. The resulting alignment was used to develop a genetic assay to differentiate between these species.
authors.affiliation: Department of Animal Sciences, Faculty of Agricultural, Food, and Environmental Sciences, the Hebrew University of Jerusalem, Rehovot 76100, Israel. dzikowsk@agri.huji.ac.il
authors.forename: R
authors.initials: R
authors.lastname: Dzikowski
authors.forename: M G
authors.initials: MG
authors.lastname: Levy
authors.forename: M F
authors.initials: MF
authors.lastname: Poore
authors.forename: J R
authors.initials: JR
authors.lastname: Flowers
authors.forename: I
authors.initials: I
authors.lastname: Paperna
mesh_terms: D000818:Animals; D001483:Base Sequence; D001715:Bird Diseases / Q000469:parasitology*; D001717:Birds; D003530:Cyprinidae / Q000469:parasitology; D017931:DNA Primers / Q000737:chemistry; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics*; D005393:Fish Diseases / Q000469:parasitology* / Q000635:transmission; D014644:Genetic Variation; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic; D016415:Sequence Alignment / Q000662:veterinary; D013045:Species Specificity; D014200:Trematoda / Q000145:classification* / Q000235:genetics; D014201:Trematode Infections / Q000469:parasitology / Q000662:veterinary*
chemical_list: D017931:DNA Primers; D018509:DNA, Helminth; D004275:DNA, Ribosomal