Expression of Bax in yeast affects not only the mitochondria but also vacuolar integrity and intracellular protein traffic.
Bax-induced lethality in yeast is accompanied by morphological changes in mitochondria, giving rise to a reduced number of swollen tubules. Although these changes are completely abolished upon coexpression of the Bax inhibitor, Bcl-2, coexpression of Bax with Bax inhibiting-glutathione S-transferase (BI-GST) leads to aggregation, but not fusion of the mitochondria. In addition, Bax affects the integrity of yeast vacuoles, resulting in the disintegration and eventual loss of the organelles, and the disruption of intracellular protein traffic. While Bcl-2 coexpression only partially corrects this phenotype, coexpression of BI-GST fully restores the organelles, indicating a different mode of protection exerted by Bcl-2 and BI-GST.
Dimitrova
Irina
I
Department of Natural Products, Mediterranean Agronomic Institute of Chania, P.O. Box 85, Chania 73100, Greece.
Toby
Garabet G
GG
Tili
Esmerina
E
Strich
Randy
R
Kampranis
Sotirios C
SC
Makris
Antonios M
AM
D002470:Cell Survival; D015870:Gene Expression; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins; D007425:Intracellular Membranes / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D021381:Protein Transport; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism* / Q000648:ultrastructure; D015003:Yeasts / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D051028:bcl-2-Associated X Protein
D008164:Luminescent Proteins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; D051028:bcl-2-Associated X Protein; D049452:Green Fluorescent Proteins; D005982:Glutathione Transferase