title: Identification of CD4+ T cell epitopes of Taenia solium paramyosin.
abstract: T cell mediated response is involved in a protective immune response against experimental cysticercosis conferred by immunization with Taenia solium paramyosin (TPmy) to BALB/c mice. In this study, we analysed the TPmy amino acid sequence for predicted CD4+ T cells epitopes. Five different regions of this protein showed that the residues anchor to bind the I-Ad molecule, synthetic peptides containing these epitopes were evaluated for their ability to induce lymphoproliferative responses of spleen cells from TPmy immunized mice. Among them, Tp176 (amino acids 176-192 sequence DDLQRQMADANSAKSRL) was the immunodominant T cell epitope of TPmy. Delineation of this epitope should facilitate analysis of the role of CD4+ T cell response in experimental cysticercosis.
authors.affiliation: Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del IPN, D.F., México, DF.
authors.forename: H S
authors.initials: HS
authors.lastname: López-Moreno
authors.forename: D
authors.initials: D
authors.lastname: Correa
authors.forename: J P
authors.initials: JP
authors.lastname: Laclette
authors.forename: V F
authors.initials: VF
authors.lastname: Ortiz-Navarrete
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002455:Cell Division / Q000276:immunology; D003551:Cysticercosis / Q000276:immunology*; D018604:Epitope Mapping; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry* / Q000276:immunology; D005260:Female; D000949:Histocompatibility Antigens Class II; D016056:Immunodominant Epitopes / Q000737:chemistry / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D041201:Taenia solium / Q000276:immunology*; D014335:Tropomyosin / Q000276:immunology*
chemical_list: D018984:Epitopes, T-Lymphocyte; D000949:Histocompatibility Antigens Class II; C111389:I-Ad antigen; D016056:Immunodominant Epitopes; D010446:Peptide Fragments; D014335:Tropomyosin