Syntrophomonas curvata sp. nov., an anaerobe that degrades fatty acids in co-culture with methanogens.
A strict anaerobe (strain GB8-1(T)) that degraded straight-chain fatty acids with C(4)-C(18) in syntrophic association with methanogens was isolated from an up-flow anaerobic sludge blanket reactor treating beer wastewater. Strain GB8-1(T) degraded 1 mol butyrate into about 2 mol acetate and 1 mol (presumably) H(2) in co-culture with a methanogen. Neither branched-chain fatty acids nor benzoate could be degraded. Strain GB8-1(T) could grow on crotonate in pure culture and converted 1 mol crotonate to 0.5 mol butyrate and 1 mol acetate. Generation time was about 11 h when grown on crotonate at 37 degrees C. Fumarate, sulfate, thiosulfate, sulfur and nitrate could not serve as electron acceptors for strain GB8-1(T) to degrade butyrate. Cells of strain GB8-1(T) were curved rods with Gram-negative cell walls; no spores were observed. The DNA G+C content was 46.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GB8-1(T) was related most closely to the fatty acid-oxidizing, syntrophic bacterium Syntrophomonas sapovorans DSM 3441(T); however, the relationship was not very close (95.4 % sequence similarity). Some phenotypic features also differentiated strain GB8-1(T) from Syntrophomonas sapovorans DSM 3441(T). Therefore, a novel species, Syntrophomonas curvata sp. nov., is proposed. The type strain is GB8-1(T) (=CGMCC 1.5010(T)=DSM 15682(T)).
Zhang
Chunyang
C
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P.R. China.
Liu
Xiaoli
X
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P.R. China.
Dong
Xiuzhu
X
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P.R. China.
D001421:Bacteria, Anaerobic / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D018920:Coculture Techniques; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism*; D008697:Methane / Q000378:metabolism; D017014:Methanobacterium / Q000378:metabolism; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D008697:Methane