Dendritic cells from chronic hepatitis B patients can induce HBV antigen-specific T cell responses.
OBJECTIVE
To determine whether dendritic cells (DCs) from chronic hepatitis B patients could induce HBV antigen-specific T cell responses or not.


METHODS
DCs were generated from peripheral blood mononuclear cells of patients with chronic hepatitis B (CHB) infection and healthy donors. We compared the phenotypes of these DCs and their ability to secrete cytokines and to participate in mixed lymphocyte reactions. In addition, autologous lymphocytes were cultured with DCs loaded with HBV core region peptide HBcAg8-27, an epitope recognized by cytotoxic T lymphocytes (CTL), and bearing human leucocyte antigen (HLA)-A2 for 10 d. Cytokine secretion and lytic activity against peptide-pulsed target cells were assessed.


RESULTS
DCs with typical morphology were generated successfully by culturing peripheral blood mononuclear cells (PBMCs) from CHB patients with AIM-V containing GM-CSF and IL-4. Compared with DCs from normal donors, the level of CD80 expressed in DCs from CHB patients was lower, and DCs from patients had lower capacity of stimulate T cell proliferation. When PBMCs isolated from patients with chronic or acute hepatitis B infection and from normal donors were cocultured with HBcAg18-27 peptide, the antigen-specific memory response of PBMCs from acute hepatitis B patients was stronger than that of PBMCs from chronic hepatitis B patients or normal donors. PBMCs cocultured with DCs treated with HBcAg18-27 CTL epitope peptide induced an antigen-specific T cell reaction, in which the level of secreted cytokines and lytic activity were higher than those produced by memory T cells.


CONCLUSIONS
DCs from patients with CHB can induce HBV antigen-specific T cell reactions, including secretion of cytokines essential for HBV clearance and for killing cells infected with HBV.
Li
Ruo-Bing
RB
Hepatology Institute, People's Hospital, Peking University, Beijing 100044, China.
Chen
Hong-Song
HS
Xie
Yao
Y
Fei
Ran
R
Cong
Xu
X
Jiang
Dong
D
Wang
Song-Xia
SX
Wei
Lai
L
Wang
Yu
Y
D000328:Adult; D000954:Antigens, Surface / Q000276:immunology; D000970:Antineoplastic Agents / Q000494:pharmacology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002450:Cell Communication / Q000276:immunology; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology* / Q000821:virology*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005260:Female; D015789:HLA-A2 Antigen / Q000276:immunology / Q000494:pharmacology; D019694:Hepatitis B, Chronic / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology; D008297:Male; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D000954:Antigens, Surface; D000970:Antineoplastic Agents; D018984:Epitopes, T-Lymphocyte; D015789:HLA-A2 Antigen; D014409:Tumor Necrosis Factor-alpha