[Study on immunoreaction induced by DCs loaded with hepatocarcinoma antigen peptide in-vitro].
OBJECTIVE
To investigate the potency and antitumor effect of specific cytotoxic T lymphocytes induced by hepatocarcinoma antigen peptide EPVTKAEML-loaded dendritic cells (DCs).


METHODS
The healthy donors of HLA-B7 genotype were selected by PCR-SSP. Isolated and cultured DCs from HLA-B7 donors' spleen tissues were loaded with the peptide EPVTKAEML from hepatocarcinoma cell line HHCC. The specific CTL reaction and inhibitory effect of anti-HLA-I molecule mAb on CTL cytotoxicity were detected in-vitro by (51)Cr-release assay.


RESULTS
4 donors with HLA-B7 genotype were found. Their spleen T lymphocytes could be induced to proliferate and differentiate into specific CTLs by DCs loaded with the peptide. Antibody-blocking test demonstrated that the cytotoxity of CTLs was strictly limited by MHC-I molecule.


CONCLUSIONS
EPVTKAEML-loaded DCs can induce efficient and specific antitumor immune reaction in-vitro.
Qu
Ping
P
Department of Pathology, Fourth Military Medical University,Xi'an 710032 China.
Sui
Yan-Fang
YF
Guo
Ai-Lin
AL
Cao
Yun-Xin
YX
Dong
Hai-Long
HL
Lu
Shao-Ying
SY
Ye
Jing
J
Zhang
Xiu-Min
XM
D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D045744:Cell Line, Tumor; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000276:immunology; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D010455:Peptides