title: Increased invasion and expression of MMP-9 in human colorectal cell lines by a CD44-dependent mechanism.
abstract: BACKGROUND
  In order to investigate the interactions between MMPs and CD44 we stably transfected a non-invasive colon cell line, SW480 with the cDNA for MMP-9 and investigated the effect on CD44 expression and in vitro invasion and migration.
  
  
  METHODS
  In vitro invasion and migration assays were carried out using Biocoat matrigel invasion chambers. MMP and CD44 expression was determined using zymography, Western blot analysis and RT-PCR.
  
  
  RESULTS
  Transfection of the parental SW480 cells with the cDNA for MMP-9 (SW480M9) caused increased invasion and migration. MMP-9 expression increased when the SW480M9 cells were grown on HA and collagen and cultured in the presence of a CD44-activating antibody. Treatment of the cells with HA and a CD44-activating antibody also resulted in increased invasion, migration and attachment.
  
  
  CONCLUSIONS
  These results demonstrated that CD44 and MMP interactions are important in controlling tumour cell invasion and migration.
authors.affiliation: School of Biotechnology, National Institute for Cellular Biotechnology, Dublin City University, Dublin 9, Ireland.
authors.forename: David
authors.initials: D
authors.lastname: Murray
authors.forename: Mary
authors.initials: M
authors.lastname: Morrin
authors.forename: Susan
authors.initials: S
authors.lastname: McDonnell
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis* / Q000378:metabolism; D006820:Hyaluronic Acid / Q000378:metabolism / Q000494:pharmacology; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D009361:Neoplasm Invasiveness; D014162:Transfection; D015854:Up-Regulation
chemical_list: D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid; D020780:Matrix Metalloproteinase 9