title: Proposal to accommodate Burkholderia cepacia genomovar VI as Burkholderia dolosa sp. nov.
abstract: Phenotypic and genotypic studies revealed new tools for differentiating Burkholderia cepacia genomovar VI from Burkholderia multivorans and other B. cepacia-complex species. Hence, the name Burkholderia dolosa sp. nov. is proposed, with LMG 18943(T) (=CCUG 47727(T)) as the type strain. B. dolosa can be differentiated from other B. cepacia-complex bacteria by its inability to assimilate tryptamine, azelaic acid and salicin and by its failure to grow on the B. cepacia-selective medium PCAT. Both 16S rDNA and recA RFLP analysis revealed unique B. dolosa restriction patterns. In addition, new 16S rDNA- and recA-based PCR assays allowed its specific identification.
authors.affiliation: Laboratory for Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium.
authors.forename: Karen
authors.initials: K
authors.lastname: Vermis
authors.affiliation: Laboratory for Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Tom
authors.initials: T
authors.lastname: Coenye
authors.affiliation: Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI 48109-0646, USA.
authors.forename: John J
authors.initials: JJ
authors.lastname: LiPuma
authors.affiliation: Cardiff School of Biosciences, PO Box 915, Cardiff University, Cardiff CF10 3TL, UK.
authors.forename: Eshwar
authors.initials: E
authors.lastname: Mahenthiralingam
authors.affiliation: Laboratory for Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium.
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Nelis
authors.affiliation: Laboratory for Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
mesh_terms: D001483:Base Sequence; D019117:Burkholderia / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D016956:Burkholderia cepacia / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D011938:Rec A Recombinases