Human ACAT-1 and -2 inhibitory activities of saucerneol B, manassantin A and B isolated from Saururus chinensis.
The sesquineolignan, saucerneol B (1), and dineolignans, manassantin A (2), and manassantin B (3), were isolated from the methanol extracts of Saururus chinensis root and elucidated by their spectroscopic data analysis. Compounds 1-3 inhibited hACAT-1 and hACAT-2 with IC(50) values of 43.0 and 124.0 microM for 1, of 39.0 and 8.0 microM for 2, of 82.0 microM and only 32% inhibition at 1mM for 3, respectively. The EtOAc-soluble fraction, which contained compounds 1-3, of methanol extracts of S. chinensis exhibited strong cholesterol-lowering effect in high cholesterol-fed mice.
Lee
Woo Song
WS
Korea Research Institute of Bioscience and Biotechnology, 52 Oun, Yusong, Daejeon 305-333, Republic of Korea.
Lee
Dae-Woo
DW
Baek
Young-Il
YI
An
Sojin
S
An
So-Jin
SJ
Cho
Kyung-Hyun
KH
Choi
Yang-Kyu
YK
Kim
Hyoung-Chin
HC
Park
Ho-Yong
HY
Bae
Ki-Hwan
KH
Jeong
Tae-Sook
TS
D000818:Animals; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005663:Furans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots; D032134:Saururaceae; D002785:Sterol O-Acyltransferase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000097587:Sterol O-Acyltransferase 2
D004791:Enzyme Inhibitors; D005663:Furans; D017705:Lignans; D010936:Plant Extracts; D002785:Sterol O-Acyltransferase; D000097587:Sterol O-Acyltransferase 2; C482885:manassantin B; C446969:saucerneol B; C054895:manassantin A; C493492:sterol O-acyltransferase 1