title: In vivo and in vitro permeability of one-step self-etch adhesives.
abstract: Adhesive dentistry should effectively restore the peripheral seal of dentin after enamel removal. We hypothesize that non-rinsing, simplified, one-step self-etch adhesives are effective for minimizing dentin permeability after tooth preparation procedures. Crown preparations in vital human teeth were sealed with Adper Prompt, Xeno III, iBond, or One-Up Bond F. Epoxy resin replicas were produced from polyvinyl siloxane impressions for SEM examination. Dentin surfaces from extracted human teeth were bonded with these adhesives and connected to a fluid-transport model for permeability measurements and TEM examination. Dentinal fluid droplets were observed from adhesive surfaces in resin replicas of in vivo specimens. In vitro fluid conductance of dentin bonded with one-step self-etch adhesives was either similar to or greater than that of smear-layer-covered dentin. TEM revealed water trees within the adhesives that facilitate water movement across the polymerized, highly permeable adhesives. Both in vitro and in vivo results did not support the proposed hypothesis.
authors.affiliation: Department of Dental Science, University of Bologna, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Chersoni
authors.forename: P
authors.initials: P
authors.lastname: Suppa
authors.forename: S
authors.initials: S
authors.lastname: Grandini
authors.forename: C
authors.initials: C
authors.lastname: Goracci
authors.forename: F
authors.initials: F
authors.lastname: Monticelli
authors.forename: C
authors.initials: C
authors.lastname: Yiu
authors.forename: C
authors.initials: C
authors.lastname: Huang
authors.forename: C
authors.initials: C
authors.lastname: Prati
authors.forename: L
authors.initials: L
authors.lastname: Breschi
authors.forename: M
authors.initials: M
authors.lastname: Ferrari
authors.forename: D H
authors.initials: DH
authors.lastname: Pashley
authors.forename: F R
authors.initials: FR
authors.lastname: Tay
mesh_terms: D000134:Acid Etching, Dental; D000328:Adult; D020770:Compomers / Q000737:chemistry; D003442:Crowns; D003806:Dentin Permeability / Q000502:physiology*; D017220:Dentin-Bonding Agents / Q000737:chemistry* / Q000145:classification; D019234:Dentinal Fluid / Q000737:chemistry; D015194:Diffusion Chambers, Culture; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D016628:Replica Techniques; D019279:Resin Cements / Q000737:chemistry; D016369:Smear Layer; D019246:Tooth Preparation; D014867:Water / Q000737:chemistry
chemical_list: D020770:Compomers; D017220:Dentin-Bonding Agents; D008689:Methacrylates; C446260:One-Up-Bond F; D019279:Resin Cements; C407402:Unifil Bond; C417842:Xeno compomer; C477386:iBond; D014867:Water