title: Analysis of proteins by CE, CIEF, and microfluidic devices with whole-column-imaging detection.
abstract: The recently developed whole-column-imaging detection technique for capillary electrophoresis (CE) and capillary isoelectric focusing (CIEF), a commercial whole-column-imaged CIEF instrument and its standard operation protocol are introduced. Furthermore, new developments and applications of whole-column-imaging detection in protein-protein interaction study, in protein separation using microfluidic devices and CIEF methods without carrier ampholytes, as well as in 2D separation techniques are reviewed. Miniaturization of whole-column-imaging CIEF and axially illuminated fluorescence whole-column-imaging CIEF are also discussed.
authors.affiliation: Convergent Bioscience Ltd., Toronto, Ontario, Canada.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Wu
authors.forename: Xing-Zheng
authors.initials: XZ
authors.lastname: Wu
authors.forename: Tiemin
authors.initials: T
authors.lastname: Huang
authors.forename: Janusz
authors.initials: J
authors.lastname: Pawliszyn
mesh_terms: D055516:2S Albumins, Plant; D000485:Allergens; D000818:Animals; D052179:Antigens, Plant; D010367:Arachis / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods; D006023:Glycoproteins / Q000737:chemistry*; D049452:Green Fluorescent Proteins; D007074:Immunoglobulin G / Q000737:chemistry*; D007525:Isoelectric Focusing / Q000379:methods; D008164:Luminescent Proteins / Q000737:chemistry; D008565:Membrane Proteins; D010799:Phycoerythrin / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D011485:Protein Binding / Q000502:physiology; D011817:Rabbits
chemical_list: D055516:2S Albumins, Plant; D000485:Allergens; D052179:Antigens, Plant; C500889:Ara h 1 protein, Arachis hypogaea; C078642:Ara h II protein, Arachis hypogaea; D006023:Glycoproteins; D007074:Immunoglobulin G; D008164:Luminescent Proteins; D008565:Membrane Proteins; D010940:Plant Proteins; D010799:Phycoerythrin; D049452:Green Fluorescent Proteins