title: Presence of translation elongation factor-1A in the rat cerebellar postsynaptic density.
abstract: This study examined a 55 kDa protein in the rat cerebellar postsynaptic density (PSD) fraction. The amino acid sequence of an HPLC-purified peptide derived from tryptic digestion of the protein was contained in eukaryotic translation elongation factor-1A (eEF1A, formerly known as eEF-1alpha). Immunoblot analysis showed that eEF1A is enriched in the PSD fraction and is tightly associated with the PSD 'core'. The association of eEF1A with the PSD was further evidenced by colocalization of the protein with PSD95, a PSD marker, in dissociated cerebellar cultures and immunoelectron microscopy of the adult rat cerebellum. Combined, our results indicate that eEF1A is associated with the PSD.
authors.affiliation: Department of Anatomy, College of Medicine, Dongguk University, 707 Sukjang, Gyeongju 780-714, South Korea. moonis@dongguk.ac.kr
authors.forename: Il Soo
authors.initials: IS
authors.lastname: Moon
authors.forename: Sun-Jung
authors.initials: SJ
authors.lastname: Cho
authors.forename: Jae Seob
authors.initials: JS
authors.lastname: Jung
authors.forename: In Sick
authors.initials: IS
authors.lastname: Park
authors.forename: Duk Kyu
authors.initials: DK
authors.lastname: Kim
authors.forename: Jin Taek
authors.initials: JT
authors.lastname: Kim
authors.forename: Bok Hyun
authors.initials: BH
authors.lastname: Ko
authors.forename: IngNyol
authors.initials: I
authors.lastname: Jin
mesh_terms: D000818:Animals; D002452:Cell Count / Q000379:methods; D002531:Cerebellum / Q000737:chemistry* / Q000648:ultrastructure; D020648:Peptide Elongation Factor 1 / Q000032:analysis*; D051381:Rats; D013569:Synapses / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D020648:Peptide Elongation Factor 1