title: Frequent occurrence of UVB-induced sister chromatid exchanges in telomere regions and its implication to telomere maintenance.
abstract: Sister chromatid exchanges (SCEs) are symmetrical exchanges between newly replicated chromatids and their sisters. While homologous recombination may be one of the principal mechanisms responsible for SCEs, the full details of their molecular basis and biological significance remain to be elucidated. Following exposure to ultraviolet light B (UVB), mitomycin C (MMC) and cisplatin, we analyzed the location of SCEs on metaphase chromosomes in Chinese hamster CHO cells. The frequency of SCEs increased over the spontaneous level in proportion to the agent's dose. UVB-induced SCEs occurred frequently in telomere regions, as cisplatin-induced SCEs did, differing from MMC-induced ones. The remarkable difference of intrachromosomal distribution among the three mutagens may be attributed to the specificity of induced DNA lesions and structures of different chromosome regions. Telomeric DNA at the end of chromosomes is composed of multiple copies of a repeated motif, 5'-TTAGGG-3' in mammalian cells. Telomeric repeats may be potential targets for UVB and cisplatin, which mainly form pyrimidine dimers and intrastrand d(GpG) cross-links, respectively, resulting in SCE formation. UVB irradiation shortened telomeres and augmented the telomerase activity. The possible implications of the frequent occurrence of SCEs in telomere regions are discussed in connection with the maintenance of telomere integrity.
authors.affiliation: Laboratory of Molecular Genetics, Biology Division, Kyoto University of Education, Fushimi, Kyoto, Japan.
authors.forename: G
authors.initials: G
authors.lastname: Jin
authors.forename: T
authors.initials: T
authors.lastname: Ikushima
mesh_terms: D000818:Animals; D041702:BALB 3T3 Cells / Q000528:radiation effects / Q000648:ultrastructure; D016466:CHO Cells / Q000528:radiation effects / Q000648:ultrastructure; D006224:Cricetinae; D003412:Cricetulus; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004790:Enzyme Induction / Q000528:radiation effects; D051379:Mice; D008807:Mice, Inbred BALB C; D016685:Mitomycin / Q000494:pharmacology; D012854:Sister Chromatid Exchange / Q000187:drug effects / Q000528:radiation effects*; D019098:Telomerase / Q000096:biosynthesis; D016615:Telomere / Q000528:radiation effects* / Q000648:ultrastructure; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D003432:Cross-Linking Reagents; D016685:Mitomycin; D019098:Telomerase