[Role of P38 mitogen-activated protein kinase in expression of P-selectin on human umbilical vein endothelial cells].
OBJECTIVE
To investigate the role of P38 mitogen-activated protein kinase (P38MAPK) in expression of P-selectin on human umbilical vein endothelial cells and diabetic atherosclerosis.


METHODS
Human umbilical vein endothelial cell line ECV304 were stimulated with high glucose, advanced glycosylation end products (AGE), high insulin and H(2)O(2) respectively, and then expression of phosphorylated-P38MAPK and P-selectin was detected after being pre-treated with SB203580 (P38MAPK specific inhibitor).


RESULTS
High glucose, AGE, high insulin and H(2)O(2) could activate P38MAPK by oneself, and increased notably expression of phosphorylated-P38MAPK and P-selectin on cell line ECV304; Expression of P-selectin was inhibited significantly by SB203580.


CONCLUSIONS
P38MAPK can regulate the expression of P-selectin, P38MAPK is an upstream signaling molecule and also may be one of initial signals on atherosclerosis occurrence.
Li
Yan-Bo
YB
Department of Endocrinology, The First Affiliated Hospital of Chongqing University of Medical Sciences, Chongqing 400016, China.
Deng
Hua-Cong
HC
Zheng
Dan
D
Li
Hu-Lun
HL
Zhou
Hong-Bo
HB
D042783:Endothelial Cells / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D019007:P-Selectin / Q000378:metabolism; D014471:Umbilical Veins; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors
D019007:P-Selectin; D006861:Hydrogen Peroxide; D048051:p38 Mitogen-Activated Protein Kinases