Halomonas ventosae sp. nov., a moderately halophilic, denitrifying, exopolysaccharide-producing bacterium.
Halomonas ventosae sp, nov. includes three moderately halophilic, exopolysaccharide-producing strains isolated from saline soils in Jaén (south-eastern Spain). These strains can grow anaerobically using either nitrate or nitrite as terminal electron acceptor and hydrolyse both tyrosine and phenylalanine. Their G+C content varies between 72.6 and 74.3 mol%. The affiliation of the isolates with the genus Halomonas was confirmed by 16S rRNA gene sequence comparison. DNA-DNA hybridization shows 70.4-82.7 % relatedness among the three strains. Nevertheless, their relatedness is less than 43 % compared to related reference strains. The proposed type strain for Halomonas ventosae is strain Al12(T) (=CECT 5797(T)=DSM 15911(T)). It grows best at 8 % (w/v) sea salts and requires the presence of Na(+). Its major fatty acids are 18 : 1 omega7c, 16 : 0, 16 : 1 omega7c, and 15 : 0 iso 2-OH. The predominant respiratory lipoquinone found in strain Al12(T) is ubiquinone with nine isoprene units (Q-9).
Martínez-Cánovas
M José
MJ
Microbial Exopolysaccharide Research Group, Department of Microbiology, Faculty of Pharmacy, Campus Universitario de Cartuja, University of Granada, 18071 Granada, Spain.
Quesada
Emilia
E
Microbial Exopolysaccharide Research Group, Department of Microbiology, Faculty of Pharmacy, Campus Universitario de Cartuja, University of Granada, 18071 Granada, Spain.
Llamas
Inmaculada
I
Microbial Exopolysaccharide Research Group, Department of Microbiology, Faculty of Pharmacy, Campus Universitario de Cartuja, University of Granada, 18071 Granada, Spain.
Béjar
Victoria
V
Microbial Exopolysaccharide Research Group, Department of Microbiology, Faculty of Pharmacy, Campus Universitario de Cartuja, University of Granada, 18071 Granada, Spain.
D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020632:Halomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D010641:Phenotype; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D009566:Nitrates; D009573:Nitrites; D011135:Polysaccharides, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S