[Induction of specific humoral immune response in mice by recombinant mutant HBV s gene vaccine].
OBJECTIVE
To construct the recombinant eukaryotic expression vector pCMV-S2. S + 145R(PR) containing mutant HBV s gene and detect the specific humoral immune response to the PR in mice.


METHODS
The PR was constructed by positional cloning of restriction endonuclease, and then it was transfected into human hepatocellular carcinoma cell line Hep G2 through electrotransformation. The antigenicity of PR was examined by EIA, ELISA and immunocytochemical staining. The PR and empty vector pcDNA3.0 were then used respectively to immunize intramuscularly 5 C57BL/6 mice, dosage being 100 pg purified plasmid each mouse. The titers of serum anti-HBs and anti-HBs2 antibodies were detected by ELISA.


RESULTS
In vitro experiment showed that mutant HBsAg could bind to anti-HBs antibody. The PR could induce anti-HBs and anti-HBs2 antibody production in immunized mice. But the appearance time of serum anti-HBs2 antibody one or two weeks earlier than that of serum anti-HBs antibody.


CONCLUSIONS
The expression product of PR had a good antigenicity, which can induce specific hu-moral immune response in C57BL/6 mice.
Ge
Jun-hui
JH
Department of Pathology, Changzheng Hospital, Shanghai 200003, China. gerllini@yahoo.com.cn
Liu
Hui-min
HM
He
Jin
J
Li
Yu-li
YL
Yu
Hong-yu
HY
Ye
Zhong
Z
Zhang
Le-zhi
LZ
Hu
Yi-ping
YP
D000818:Animals; D005260:Female; D006510:Hepatitis B Antibodies / Q000097:blood*; D006514:Hepatitis B Surface Antigens / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D017325:Hepatitis B Vaccines / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D011498:Protein Precursors / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*
D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D011498:Protein Precursors; D014614:Vaccines, Synthetic; C050807:presurface protein 2, hepatitis B surface antigen