Erythropoietin up-regulates SOCS2 in neuronal progenitor cells derived from SVZ of adult rat.
We examined the effects of EPO on expression of suppressor of cytokine signaling 2 (SOCS2) and found that treatment of neural progenitor cells derived from the adult subventricular zone (SVZ) with recombinant human EPO (rhEPO) stimulated progenitor cell differentiation into neurons, but not astrocytes. Quantitative RT-PCR revealed that SOCS2 mRNA levels were increased in the progenitor cells treated with rhEPO. Immunostaining showed that neurons but not astrocytes were SOCS2 immunoreactive. Incubation of the progenitor cells with rhEPO in the presence of a neutralizing antibody against EPO abolished the effects of EPO on neuronal differentiation and expression of SOCS2. Our data suggest that up-regulation of SOCS2 in neuronal progenitor cells derived from the adult SVZ may regulate EPO enhanced neuronal differentiation.
Wang
Lei
L
Departments of Neurology, Henry Ford Health Sciences Center, 2799 W. Grand Boulevard, Detroit, MI 48202, USA.
Zhang
Zhenggang
Z
Zhang
Ruilan
R
Hafner
Mikehl S
MS
Wong
Henry K
HK
Jiao
ZhongXian
Z
Chopp
Michael
M
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D001921:Brain / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics*; D004921:Erythropoietin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007150:Immunohistochemistry; D020547:Lateral Ventricles / Q000166:cytology / Q000254:growth & development; D008297:Male; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012097:Repressor Proteins / Q000235:genetics*; D018874:Spheroids, Cellular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D050826:Suppressor of Cytokine Signaling Proteins; D015534:Trans-Activators / Q000235:genetics*; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
D000906:Antibodies; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; C491408:Socs2 protein, rat; D050826:Suppressor of Cytokine Signaling Proteins; D015534:Trans-Activators; D004921:Erythropoietin