title: CpG island hypermethylation is maintained in human colorectal cancer cells after RNAi-mediated depletion of DNMT1.
abstract: The role of the primary mammalian DNA methyltransferase, DNMT1, in maintaining CpG island methylation in human colon cancer cells has recently been questioned. This controversy has arisen from discrepancies between genetic knockout and RNA interference-mediated knockdown studies. Here, we re-examined the RNA interference-based approach and found that hypermethylation of single-copy genes is maintained in cells transiently and stably depleted of DNMT1.
authors.affiliation: Program in Cellular and Molecular Medicine, Cancer Biology Division, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, The Johns Hopkins University, Baltimore, Maryland 21231, USA.
authors.forename: Angela H
authors.initials: AH
authors.lastname: Ting
authors.forename: Kam-wing
authors.initials: KW
authors.lastname: Jair
authors.forename: Hiromu
authors.initials: H
authors.lastname: Suzuki
authors.forename: Ray-Whay Chiu
authors.initials: RW
authors.lastname: Yen
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Baylin
authors.forename: Kornel E
authors.initials: KE
authors.lastname: Schuebel
mesh_terms: D001483:Base Sequence; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000235:genetics*; D018899:CpG Islands; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation; D020868:Gene Silencing; D016147:Genes, Tumor Suppressor; D045325:HCT116 Cells; D006801:Humans; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D012334:RNA, Neoplasm / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D012334:RNA, Neoplasm; D034741:RNA, Small Interfering; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; C000619380:DNMT1 protein, human