Distribution of adrenomedullin-containing perivascular nerves in the rat mesenteric artery.
Distribution of adrenomedullin (AM)-containing perivascular nerve fibers was studied in rat mesenteric arteries. Many fibers containing AM-like immunoreactivity (LI) were observed in the adventitia. AM-LI fibers were abolished by cold storage denervation or capsaicin but not 6-hydroxydopamine. Double immunostainings showed colocalization of AM-LI with calcitonin gene-related peptide (CGRP)-LI. The dorsal root ganglia had many AM-positive cells and AM mRNA detected by RT-PCR. Electron microscopy study revealed high proportions of immunogold labeling for AM and colocalization of both AM-LI and CGRP-LI in unmyelinated nerve axons. These results suggest that AM-containing perivascular nerves are distributed in the rat mesenteric artery.
Hobara
N
N
Department of Clinical Pharmaceutical Science, Graduate School of Natural Science and Technology, Okayama University, 1-1-1 Tsushima-naka, Okayama 700-8530, Japan.
Nakamura
A
A
Ohtsuka
A
A
Narasaki
M
M
Shibata
K
K
Gomoita
Y
Y
Kawasaki
H
H
D053607:Adrenomedullin; D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure*; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D003238:Connective Tissue / Q000294:innervation; D005727:Ganglia, Spinal / Q000378:metabolism / Q000648:ultrastructure*; D008297:Male; D008638:Mesenteric Arteries / Q000294:innervation*; D008854:Microscopy, Electron; D010455:Peptides / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D010455:Peptides; D053607:Adrenomedullin; D015740:Calcitonin Gene-Related Peptide