Cytochemical localisation of vanadium (III) in the blood cells of the ascidian Phallusia fumigata.
Most recent studies of vanadium in ascidian blood indicate the signet ring cell as the cell type that stores vanadium. However, in Phallusia fumigata the situation is less clear, with contradictory reports. Therefore, the blood cells of the ascidian P. fumigata were stained with the vanadium(III) specific ligand 2,2'-bipyridine. The presence of vanadium was revealed in the vacuolated amoebocyte, the second compartment cell type or pluri-vacuolated cell, as well as the bivacuolated cell and the signet ring cell. Staining was not observed in either the morula cells or the compartment cells, the predominant cell types in the mature animal.
Nette
Geoffrey
G
Dipartimento di Genetic Biologia, Generale E Molecolare, Facolta di Scienze, Università di Napoli, via Mezzacanone 8, I-80134 Naples, Zoological Station 'A. Dohrn', Villa comunale, 80121 Naples, Italy.
Scippa
Silvia
S
De Candia
Angela
A
de Vincentiis
Mario
M
D015082:2,2'-Dipyridyl; D000818:Animals; D006434:Hemocytes / Q000737:chemistry*; D006651:Histocytochemistry; D007202:Indicators and Reagents; D008024:Ligands; D013194:Staining and Labeling; D014561:Urochordata / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism; D014639:Vanadium / Q000097:blood* / Q000378:metabolism*
D007202:Indicators and Reagents; D008024:Ligands; D014639:Vanadium; D015082:2,2'-Dipyridyl