title: Physiological and pathological age-associated changes in diurnal rhythm of energy expenditure in rats.
abstract: Although the idea that energy metabolism of rats decreases with age has been widely accepted, few studies with regard to the diurnal rhythm of energy expenditure have been reported. Whether age alone altered the diurnal rhythm of energy expenditure was examined in Sprague-Dawley (SD) rats. The same determination was conducted in Otsuka Long Evans Tokushima Fatty (OLETF) rats to examine the effect of insulin resistance and diabetes. OLETF rats were developed as a model of non-insulin-dependent diabetes mellitus (NIDDM) with mild obesity. The characteristic features of OLETF rats are late onset of hyperglycemia at about 18 weeks of age, followed by insulin deficiency at about 65 weeks. Age-associated changes in diurnal rhythm of energy expenditure were not observed in SD rats. In OLETF rats, the diurnal rhythm of energy expenditure with two peaks was observed at 8 weeks of age, while these two peaks disappeared at 24 weeks of age (with NIDDM). Then, the pattern of diurnal rhythm at 44 weeks of age (with advanced NIDDM) was resembled to that of 62 weeks of age (with insulin deficiency). In conclusion, we clarified the changes in diurnal rhythm of energy expenditure associated with the progress of diabetes, while age alone did not alter the diurnal rhythm.
authors.affiliation: Department of Clinical Physiology, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakaecho, Itabashi-ku, Tokyo 173-0015, Japan. miyasaka@tmig.or.jp
authors.forename: Kyoko
authors.initials: K
authors.lastname: Miyasaka
authors.forename: Mineko
authors.initials: M
authors.lastname: Ichikawa
authors.forename: Kiyokazu
authors.initials: K
authors.lastname: Momose
authors.forename: Atsushi
authors.initials: A
authors.lastname: Araki
authors.forename: Mikako
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Yuhei
authors.initials: Y
authors.lastname: Ichimaru
authors.forename: Akihiro
authors.initials: A
authors.lastname: Funakoshi
mesh_terms: D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D002940:Circadian Rhythm; D003920:Diabetes Mellitus / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D004734:Energy Metabolism / Q000502:physiology*; D009765:Obesity; D051381:Rats; D020334:Rats, Inbred OLETF; D017207:Rats, Sprague-Dawley