[Rapid detection of the genotyping of hepatitis C virus using DNA chip with coloration methods].
OBJECTIVE
To develop a new DNA chip with coloration, which can be used for rapid and economical detection of the genotyping of hepatitis C virus (HCV).


METHODS
Probes and primers were designed according to the sequence of HCV 5' non-coding region (5' NCR) to fabricate DNA chip. Experimental group consisted of 60 positive serum samples and control group consisted of 20 negative serum samples. To obtain the aimed gene, then they were hybridized with DNA chip. Finally, the results showed in a nylon film. The results of DNA sequencing of samples were used as the control in double blind experimental.


RESULTS
Using DNA chip, HCV was detected in positive of all serum specimens of experimental group and negative in control group. The determination of HCV genotype by DNA chip showed corresponding rate of 96.7% with those by sequence assay.


CONCLUSIONS
It showed higher specialty and sensitivity using DNA chip to detect the genotype of HCV. It would be valuable for the clinical genotyping of HCV
Zhang
Yi-guan
YG
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
Mao
Hong-ju
HJ
Gu
Shi-min
SM
Xu
Bao-jian
BJ
Zhao
Jian-long
JL
Dong
Zi-ming
ZM
D020121:5' Untranslated Regions / Q000235:genetics; D001483:Base Sequence; D005838:Genotype; D016174:Hepacivirus / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D017422:Sequence Analysis, DNA
D020121:5' Untranslated Regions