[Enzymes and processes of activation of the endometrium stromal cells].
The endometrium stroma cells and properties of such key enzymes as acetylcholinesterase, Mg2+, Ca(2+)-ATPase, AMP-deaminase have been investigated in them. The activity of acetylcholinesterase in suspension of cells compounds is 9.8 +/- 0.2 mumol of tiocholinbromide/mg protein/hour and is reduced under influence of exogenous ATP, NO2-, H2O2 and Triton X-100. Common Mg2+, Ca(2+)-ATPase activity of compounds of 36 +/- 2 mumol Pi/mg protein/hour, is depressed by sodium azide and thapsigargine, that specifies presence of an investigated enzyme in mitochondria and endoplasmic reticulum of investigated cells. In a suspension of stroma cells with addition of 0.2% of Triton X-100 for augmentation of permeability of cellular membranes and 1.5 M KCl for a dissociation of complexes AMP-deaminase with proteins and membranes, the deamination exogenous AMP up to IMP and NH3, is registered generated in the given response. The supposition about NH3 role as the paracrine regulator in the system endometrium-myometrium of the uterus is expressed.
Tuhaĭ
V A
VA
Palladin Institute of Biochemistry, National Academy of Science of Ukraine, Kyiv.
Danylovych
Iu V
IuV
D000659:AMP Deaminase / Q000378:metabolism; D000110:Acetylcholinesterase / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D003470:Culture Media; D003641:Deamination; D004717:Endometrium / Q000166:cytology* / Q000201:enzymology* / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000201:enzymology* / Q000378:metabolism; D005260:Female; D008928:Mitochondria / Q000201:enzymology* / Q000378:metabolism; D017154:Stromal Cells / Q000201:enzymology / Q000378:metabolism; D013552:Swine
D003470:Culture Media; D000641:Ammonia; D000110:Acetylcholinesterase; D000659:AMP Deaminase; D017301:Ca(2+) Mg(2+)-ATPase; D002118:Calcium