[Effect of insulin on apoptosis of cultured human trophoblast cells and its mechanism].
OBJECTIVE
To explore the effect of insulin on apoptosis of cultured human trophoblast cells and its possible mechanism.


METHODS
Human trophoblast cells from early pregnancy women were cultured and divided into 3 groups; normal control group; H2O2, treatment group and insulin plus H2O2 treatment group. H2O2 was used to induce apoptosis of trophoblasts cells. Apoptotic rate was detected by flow cytometry. The effects of insulin on Bcl-2 expression and caspase-3 activity were also detected.


RESULTS
H2O2 might induce apoptosis of trophoblast cells and typical morphological features of apoptotic cells was observed under electron microscope. Flow cytometry detection exhibited that insulin could reduce markedly H2O2-induced apoptotic rate of trophoblasts cells (P < 0. 01). Bcl-2 expression rate inH,O, treatment group was significantly lower than that in control group (P < 0. 01), while caspase-3 activity was distinctly higher than that in control group (P < 0. 01).


CONCLUSIONS
Insulin could inhibit apoptosis of human trophoblasts cells induced by H2O2, which be may through decreased caspase-3 activity and increased Bcl-2 protein expression.
Yu
Yue-cheng
YC
Department of Obstetrics and Gynecology, Xijing Hospital, China.
Xin
Xiao-yan
XY
Zhang
Feng
F
Ma
Xiang-dong
XD
Wang
De-tang
DT
Guo
Hui-ling
HL
D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D007633:Keratins / Q000032:analysis; D011247:Pregnancy; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D014327:Trophoblasts / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure
D007328:Insulin; D019253:Proto-Oncogene Proteins c-bcl-2; D007633:Keratins; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases