title: The influence of methyl jasmonate and beta-glucosidase on induction of apple tree resistance mechanisms to two-spotted spider mite (Tetranychus urncae Koch.).
abstract: The objective of the research was to determine the influence of methyl jasmonate (JA-Me) and beta-glucosidase on the mechanisms of apple tree resistance to T. urticae like antibiosis and non-preference. The experiments were conducted on leaves of Close and Jester apple cultivars in laboratory conditions. Leaves were treated with: 1. solution of 0.1% JA-Me in 0.05% Triton X-100 (by spraying); 2. beta-glucosidase dissolved in 0.1 M citrate buffer at pH 6 (by petiole); 3. 0.05% solution of the Triton X-100 (by spraying); 4. 0.1 M citrate buffer at pH 6 (by petiole); 5. non-treated leaves. In the no-choice test, application of JA-Me on leaves of apple trees caused reducing of number of eggs laid up during three days of the experiment. In the choice test, which was carried out for determination of non-preference mechanism of resistance, there was not significant differences between number of mites on leaves treated with JA-Me compared to leaves treated with beta-glucosidase, and to non-treated leaves after 24 hours from solutions application. However, at the same experiment, females of T. urticae laid the least number of eggs on leaves treated with JA-Me. Analysis conducted using liquid chromatography method, revealed higher level of phenolic compounds on leaves treated with JA-Me compared to check and leaves treated with beta-glucosidase. Thus, it appears that JA-Me may be regarded as elicitor of induced resistance of apple tree to two-spotted spider mite.
authors.affiliation: Research Institute of Pomology and Floriculture Pomologiczna 18 Str. 96-100 Skiemiewice, Poland. wwarab@insad.pl
authors.forename: W
authors.initials: W
authors.lastname: Warabieda
authors.forename: A
authors.initials: A
authors.lastname: Miszczak
authors.forename: R W
authors.initials: RW
authors.lastname: Olszak
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000818:Animals; D003517:Cyclopentanes / Q000494:pharmacology*; D007113:Immunity, Innate; D027845:Malus / Q000201:enzymology* / Q000469:parasitology*; D008925:Mites / Q000472:pathogenicity*; D054883:Oxylipins; D010572:Pest Control, Biological / Q000379:methods*; D010636:Phenols / Q000378:metabolism; D010935:Plant Diseases / Q000469:parasitology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000378:metabolism; D011044:Poland; D001617:beta-Glucosidase / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010636:Phenols; D010937:Plant Growth Regulators; C072239:methyl jasmonate; D001617:beta-Glucosidase