title: [Role of p38 mitogen-activated protein kinase signal transduction pathway in the acute lung injury of severely burned rats].
abstract: OBJECTIVE
  To investigate the role of p38 mitogen-activated protein kinase (MAPK) signal transduction pathway in the acute lung injury of severely burned rats.
  
  
  METHODS
  Forty-eight adult healthy rats were randomly divided into three groups: sham group, burn control group, and burn + SB203580 group. A third-degree burns over 30% total body surface area rat model was used and pulmonary capillary permeability, lung water content, pulmonary histology and p38 MAPK activity were measured at 24 hours postburn.
  
  
  RESULTS
  Burn trauma resulted in increased pulmonary capillary leakage permeability (42.5 +/- 4.7 vs. 12.1 +/- 1.4, P < 0.01), elevated lung water content (P < 0.05), and worsen histologic condition. There was a significant activation of p38 MAPK at 24 hours postburn compared with control. SB203580 inhibited the activation of p38 MAPK, reduced the pulmonary capillary leakage permeability (24.7 +/- 2.9 vs. 42.5 +/- 4.7, P < 0.01), decreased lung water content, and prevented burn-mediated lung injury.
  
  
  CONCLUSIONS
  The activation of p38 MAPK is one important aspect of the signaling event that contributes to burn-induced lung injury.
authors.affiliation: Department of Burns, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Xu-lin
authors.initials: XL
authors.lastname: Chen
authors.forename: Zhao-fan
authors.initials: ZF
authors.lastname: Xia
authors.forename: Duo
authors.initials: D
authors.lastname: Wei
authors.forename: Dao-feng
authors.initials: DF
authors.lastname: Ben
authors.forename: Guang-qing
authors.initials: GQ
authors.lastname: Wang
authors.forename: Sheng
authors.initials: S
authors.lastname: Han
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002056:Burns / Q000201:enzymology / Q000503:physiopathology*; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology; D008168:Lung / Q000473:pathology / Q000503:physiopathology; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000502:physiology; D011725:Pyridines / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012128:Respiratory Distress Syndrome / Q000201:enzymology / Q000503:physiopathology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D048051:p38 Mitogen-Activated Protein Kinases
chemical_list: D004791:Enzyme Inhibitors; D007093:Imidazoles; D011725:Pyridines; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580