[Screening of bioactive peptide that mimic the epitope of gastric cancer associated antigen].
OBJECTIVE
To screen bioactive peptides that mimic the epitope of gastric cancer associated antigen.


METHODS
Anti-gastric cancer monoclonal antibody (mAb) MG7 was purified by ion chromatography, and then coated on ELISA plate. By using the mAb as selective molecular, a 12-meres phage displaying peptide library was biopanned, and the positive clones were selected.


RESULTS
12 positive phage clones were selected.


CONCLUSIONS
A candidate mimic epitope of gastric cancer associated antigen was identified, which provided the foundation for developing tumor peptide vaccine.
Hang
Quan-Li
QL
Department of Gastroenterology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Ding
Jie
J
Gong
Ai-Chun
AC
Yu
Zhao-Cai
ZC
Qiao
Tai-Dong
TD
Chen
Bao-Jun
BJ
Zhang
Xue-Yong
XY
Fan
Dai-Ming
DM
D001435:Bacteriophages / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D006801:Humans; D019151:Peptide Library; D013274:Stomach Neoplasms / Q000276:immunology
D000939:Epitopes; D019151:Peptide Library