title: Correlation of allergen-induced IL-5 and IL-13 production by peripheral blood T cells of asthma patients.
abstract: BACKGROUND
  Helper T cells and T cell cytokines are implicated in allergic disorders such as asthma and atopic dermatitis (AD). We reported enhanced interleukin-5 (IL-5) production by peripheral blood T cells of asthmatic patients. Production of cytokines, particularly IL-5 and IL-13, by peripheral blood mononuclear cells (PBMC) obtained from bronchial asthma (BA) and AD patients was investigated in the present study.
  
  
  METHODS
  PBMC were cultured in the presence of either polyclonal activator, phorbol ester plus Ca2+ ionophore or Dermatophagoides farinae (Df) antigen. The resulting supernatants were assayed for IL-4, IL-5, and IL-13 by specific ELISAs.
  
  
  RESULTS
  IL-5 and IL-13 production in response to Df antigen is significantly higher in allergic groups compared to control subjects. IL-5 responses induced by Df antigen were strongly correlated with IL-13 responses.
  
  
  CONCLUSIONS
  In spite of IL-13 gene proximity to IL-4 gene, IL-5 production was more strongly correlated to IL-13 production in the BA group, suggesting a common control mechanism that regulates the IL-5 and IL-13 gene.
authors.affiliation: Clinical Research Center for Allergy and Rheumatology, National Sagamihara Hospital, Kanagawa, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Hashimoto
authors.forename: Kazuo
authors.initials: K
authors.lastname: Akiyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawaguchi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Maeda
authors.forename: Masami
authors.initials: M
authors.lastname: Taniguchi
authors.forename: Noriaki
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Akio
authors.initials: A
authors.lastname: Mori
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology; D039741:Antigens, Dermatophagoides / Q000276:immunology; D001249:Asthma / Q000276:immunology*; D002478:Cells, Cultured; D003876:Dermatitis, Atopic / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D018793:Interleukin-13 / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000096:biosynthesis; D015848:Interleukin-5 / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D018793:Interleukin-13; D015848:Interleukin-5; D015847:Interleukin-4