title: Decolorization of synthetic dyes by solid state cultures of Lentinula (Lentinus) edodes producing manganese peroxidase as the main ligninolytic enzyme.
abstract: The ability of the white-rot fungus Lentinula (Lentinus) edodes to decolorize several synthetic dyes was investigated using solid state cultures with corn cob as substrate. Cultures, containing amido black, congo red, trypan blue, methyl green, remazol brilliant blue R, methyl violet, ethyl violet and Poly R478 at 200 ppm, were completely decolorized after 18 days of incubation. Partial decolorization was observed in the cultures containing 200 ppm of brilliant cresyl blue and methylene blue. High manganese peroxidase activity (2600 U/g substrate), but very low lignin peroxidase (<10 U/g substrate) and laccase (<16 U/g substrate) activities were detected in the cultures. In vitro, the dye decolorization was markedly decreased by the absence of manganic ions and H2O2. These data suggest that manganese peroxidase appear to be the main responsible for the capability of L. edodes to decolorize synthetic dyes.
authors.affiliation: Department of Biochemistry, University of Maringá, 87020-900 Maringá PR, Brazil.
authors.forename: Cinthia Gandolfi
authors.initials: CG
authors.lastname: Boer
authors.forename: Larissa
authors.initials: L
authors.lastname: Obici
authors.forename: Cristina Giatti Marques
authors.initials: CG
authors.lastname: de Souza
authors.forename: Rosane M
authors.initials: RM
authors.lastname: Peralta
mesh_terms: D000704:Analysis of Variance; D001673:Biodegradation, Environmental; D003116:Color; D004396:Coloring Agents / Q000378:metabolism*; D006861:Hydrogen Peroxide; D007700:Kinetics; D010544:Peroxidases / Q000378:metabolism*; D020942:Shiitake Mushrooms / Q000201:enzymology / Q000378:metabolism*; D013997:Time Factors
chemical_list: D004396:Coloring Agents; D006861:Hydrogen Peroxide; D010544:Peroxidases; C051129:manganese peroxidase