title: In vitro activities of garenoxacin and levofloxacin against Chlamydia pneumoniae are not affected by presence of Mycoplasma DNA.
abstract: We studied 20 Chlamydia pneumoniae isolates obtained from respiratory sites and atheroma tissue of patients from various geographic areas to determine the susceptibilities of these isolates to a new des-fluoroquinolone, garenoxacin, and to levofloxacin. In addition, we assessed the cultures with these isolates by PCR for the presence or absence of Mycoplasma sp. DNA. Both the MIC at which 90% of isolates are inhibited (MIC(90)) and the minimal bactericidal concentration at which 90% of isolates are killed (MBC(90)) for garenoxacin were 0.06 microg/ml, and both the MIC(90) and the MBC(90) for levofloxacin were 2.0 microg/ml. The activity of garenoxacin against C. pneumoniae was 32-fold greater than that of levofloxacin. Mycoplasma sp. DNA was detected by PCR in 17 of 20 cultures. Mycoplasma amplicons from five Mycoplasma DNA-positive C. pneumoniae cultures were sequenced and found to represent four Mycoplasma species. Our data demonstrate that C. pneumoniae cultures frequently contain Mycoplasma DNA and that its presence in C. pneumoniae cultures does not appear to affect the susceptibility results for the two fluoroquinolones that we tested.
authors.affiliation: Stratton Veterans Affairs Medical Center, New York State Department of Health, Albany, NY 12208, USA.
authors.forename: Raymond P
authors.initials: RP
authors.lastname: Smith
authors.forename: Aldona L
authors.initials: AL
authors.lastname: Baltch
authors.forename: William J
authors.initials: WJ
authors.lastname: Ritz
authors.forename: Andrea N
authors.initials: AN
authors.lastname: Carpenter
authors.forename: Tanya A
authors.initials: TA
authors.lastname: Halse
authors.forename: Lawrence H
authors.initials: LH
authors.lastname: Bopp
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D002690:Chlamydia Infections / Q000382:microbiology; D016993:Chlamydophila pneumoniae / Q000187:drug effects*; D004269:DNA, Bacterial / Q000235:genetics*; D030541:Databases, Genetic; D024841:Fluoroquinolones / Q000494:pharmacology*; D006801:Humans; D064704:Levofloxacin; D008826:Microbial Sensitivity Tests; D009174:Mycoplasma / Q000235:genetics*; D015242:Ofloxacin / Q000494:pharmacology*; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D024841:Fluoroquinolones; D012336:RNA, Ribosomal, 16S; D064704:Levofloxacin; D015242:Ofloxacin; C419689:garenoxacin