title: [Preparation and identification of polyclonal antibody to B cell epitope domain of TRP-1].
abstract: OBJECTIVE
  To prepare the polyclonal antibody to B cell epitope domain of TRP-1 and apply it to study the immunotherapy of vitiligo and melanoma.
  
  
  METHODS
  The fusion protein PRSETA/TRP-1 was expressed in E.coli, the polyclonal antibody was prepared by immunizing a rabbit with the protein. The qulity of the antibody was identified by ELISA and Western-blot.
  
  
  RESULTS
  (1)The pRSEA/TRP-1 fusion protein was correctly expressed;(2)the polyclonal antibodies to the B cell eoitope domain of TRP-1 were obtained; (3)the rabbit antiserum containing of polyclonal antibody showed high titer and possessed good binding activity to 6His-TRP1 expressed by Pichia pastoris.
  
  
  CONCLUSIONS
  The polyclonal antibody could be applied to the research on B cell epitope domain of TRP-1.
authors.affiliation: Department of Dermatology and Venerology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Ting-Hui
authors.initials: TH
authors.lastname: Li
authors.forename: Tian-Wen
authors.initials: TW
authors.lastname: Gao
authors.forename: Chun-Ying
authors.initials: CY
authors.lastname: Li
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Hou
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Liu
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D018985:Epitopes, B-Lymphocyte; D004926:Escherichia coli / Q000378:metabolism; D007106:Immune Sera / Q000276:immunology
chemical_list: D000906:Antibodies; D018985:Epitopes, B-Lymphocyte; D007106:Immune Sera