[Inhibition of momordica anti-HIV protein of MAP30 on HBeAg expression by laser scanning confocal microscopy].
OBJECTIVE
To investigate the effect of momordica anti-HIV protein of M(r ) being 30 000 (MAP30) on HBV expression by laser scanning confocal microscopy.


METHODS
HBV DNA-transfected hepatocarcinoma cells 2.2.15 were cultured in the presence of MAP30. Then quantitative analysis of HBeAg expression in the 2.2.15 cells by laser scanning confocal microscopy after indirect immunofluorescence staining was performed.


RESULTS
The fluorescence intensity in 2.2.15 cells co-cultured with MAP30 was notably weaker than that in control cells.


CONCLUSIONS
MAP30 can effectively inhibit HBeAg expression in the 2.2.15 cells.
Wang
Jiu-Ping
JP
Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Wang
Lin-Xu
LX
Sun
Yong-Tao
YT
Bai
Xue-Fan
XF
Li
Jin-Ge
JG
Chen
Wei-Hong
WH
Sun
Li
L
Luo
Kang-Xian
KX
D002478:Cells, Cultured; D006513:Hepatitis B e Antigens; D006515:Hepatitis B virus / Q000187:drug effects; D054298:Human Immunodeficiency Virus Proteins; D006801:Humans; D018613:Microscopy, Confocal; D029775:Momordica; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2
D006513:Hepatitis B e Antigens; D054298:Human Immunodeficiency Virus Proteins; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2