The analysis of thyroxine in human serum by an 'exact matching' isotope dilution method with liquid chromatography/tandem mass spectrometry.
A method for the analysis of thyroxine in human serum, utilising 'exact matching' isotope dilution mass spectrometry (IDMS) in combination with liquid chromatography/tandem mass spectrometry (LC/MS/MS), has been developed with a limit of quantification of 0.5 ng g(-1) of thyroxine in human serum. The extraction and clean-up of thyroxine from serum involves an efficient protein-precipitation stage followed by a solid-phase extraction procedure to produce an extract essentially free from interfering compounds. The method is reproducible, with expanded uncertainties of less than 2%, and is relatively fast to perform.
Hopley
Chris J
CJ
Laboratory of the Government Chemist (LGC Limited), Queens Road, Teddington, Middlesex TW11 0LY, UK.
Stokes
Peter
P
Webb
Kenneth S
KS
Baynham
Michael
M
D001774:Blood Chemical Analysis / Q000379:methods; D002247:Carbon Isotopes; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013974:Thyroxine / Q000097:blood*
D002247:Carbon Isotopes; D013974:Thyroxine