Estrogen-inducible uterine flavonoid binding sites: is it time to reconsider?
Epidemiological data support the beneficial effect of plant flavonoids on human health including anti-inflammatory and cancer preventing actions. The phytoestrogen flavonoids might interfere with estrogen action. The possible relations between the steroid- and the flavonoid-signalling in animal and plant cells have been addressed in numerous studies in the past decade. In search for possible sites of conjunction between these phenomena the post-receptor targets must not be disregarded. The estrogen-inducible type II estrogen binding sites of rat uteri have first been reported 25 years ago by Clark and coworkers [Biochem. Biophys. Res. Commun. 81 (1978) 1]. These sites are known to bind catecholic flavonoids with considerable affinity. Behaviour of the tyrosinase-like enzymatic activity associated with these sites appeared reminiscent to the recently described dopachrome oxidase or tautomerase activity exhibited by the cytokine macrophage migration inhibitory factor (MIF) inasmuch as it also accepts a broad range of catecholic melanogenic precursors. Therefore we assessed, whether the known type II ligand flavonoids interfere with the MIF tautomerase. We report here, that luteolin and quercetin have a biphasic effect on the enol-keto conversion of phenylpyruvate mediated by MIF tautomerase. We also demonstrate the presence of MIF immunoreactivity by Western blotting in rat uterine nuclear extracts prepared according to the method that yields high type II binding activity. These data support the possible participation of MIF in type II estrogen binding phenomena.
Garai
János
J
Department of Pathophysiology, Medical School, University of Pécs, Szigeti u. 12., Pécs H-7624, Hungary. garaij@main.pote.hu
Adlercreutz
Herman
H
D000818:Animals; D015153:Blotting, Western; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D019746:Intramolecular Oxidoreductases / Q000032:analysis / Q000378:metabolism; D047311:Luteolin; D008263:Macrophage Migration-Inhibitory Factors / Q000032:analysis / Q000378:metabolism; D010667:Phenylpyruvic Acids / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011960:Receptors, Estrogen / Q000378:metabolism*; D014599:Uterus / Q000737:chemistry*
D005419:Flavonoids; D008263:Macrophage Migration-Inhibitory Factors; D010667:Phenylpyruvic Acids; D011960:Receptors, Estrogen; C107081:estrogen binding site, type II; D011794:Quercetin; D019746:Intramolecular Oxidoreductases; D047311:Luteolin; C031606:phenylpyruvic acid