Enzymatic chlorophyll degradation in wheat near-isogenic lines elicited by cereal aphid (Homoptera: Aphididae) feeding.
Chlorophyll degradation enzyme (i.e., chlorophyllase, Mg-dechelatase, and chlorophyll oxidase) activities of aphid-infested and uninfested 'Tugela' and Tugela near-isogenic wheat lines (i.e., Tugela-Dn1, Tugela-Dn2, and Tugela-Dn5) were assayed. Chlorophyllase activity was higher in bird cherry-oat aphid, Rhopalosiphum padi (L.) (Homoptera: Aphididae),-infested wheat lines compared with Russian wheat aphid, Diuraphis noxia (Mordvilko) (Homoptera: Aphididae)]-infested and uninfested plants. Mg-dechelatase activity was higher in D. noxia-infested wheat lines than in R. padi-infested and uninfested plants. Also, Mg-dechelatase activity was lower in Tugela wheat infested with D. noxia than in Tugela near-isogenic lines with Dn genes. Based on the in vitro assays of chlorophyll degradation enzyme (i.e., chlorophyllase and Mg-dechelatase) activities, we proposed that the chlorotic symptoms observed on D. noxia-infested Tugela wheat were most likely to be elicited by unbalanced chlorophyll biosynthesis and degradation.
Wang
Tao
T
Department of Entomology, Montana State University, Bozeman, MT 59717, USA.
Quisenberry
Sharron S
SS
Ni
Xinzhi
X
Tolmay
Vicki
V
D000818:Animals; D001042:Aphids / Q000502:physiology*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism*; D004435:Eating; D004798:Enzymes; D005838:Genotype; D010088:Oxidoreductases / Q000378:metabolism; D014908:Triticum / Q000201:enzymology* / Q000235:genetics*
D002352:Carrier Proteins; D004798:Enzymes; C421174:magnesium dechelatase; D002734:Chlorophyll; D010088:Oxidoreductases; D002265:Carboxylic Ester Hydrolases; C019875:chlorophyllase