Identifying functional genetic variants in DNA repair pathway using protein conservation analysis.
The role of DNA repair in initiation, promotion, and progression of malignancy suggests that variations in DNA repair genes confer altered cancer risk. Accordingly, DNA repair gene variants have been studied extensively in the context of cancer predisposition. Single nucleotide polymorphisms (SNPs) are the most common genetic variations in the human genome. A fraction of SNPs are located within the genes, which are likely to alter the gene expression and function. SNPs that change the encoded amino acid sequence of the proteins (non-synonymous; nsSNPs) are potentially genetic disease determinant variations. However, as not all amino acid substitutions are supposed to lead to a change in protein function, it will be necessary to have a priori prediction and determination of the functional consequences of amino acid substitutions per se, and then together with other genetic and environmental factors to study their possible association with a trait. Here we report the analysis of nsSNPs in 88 DNA repair genes and their functional evaluation based on the conservation of amino acids among the protein family members. Our analysis demonstrated that >30% of variants of DNA repair proteins are highly likely to affect the function of the proteins drastically. In this study, we have shown that three nsSNPs, which were predicted to have functional consequences (XRCC1-R399Q, XRCC3-T241M, XRCC1-R280H), were already found to be associated with cancer risk. The strategy developed and applied in this study has the potential to identify functional protein variants of DNA repair pathway that may be associated with cancer predisposition.
Savas
Sevtap
S
Fred A. Litwin Centre for Cancer Genetics, Mount Sinai Hospital, Samuel Lunenfeld Research Institute, 600 University Avenue Room 992A, Toronto, ON M5G 1X5, Canada.
Kim
David Y
DY
Ahmad
M Farhan
MF
Shariff
Mehjabeen
M
Ozcelik
Hilmi
H
D004260:DNA Repair / Q000235:genetics* / Q000502:physiology; D004273:DNA, Neoplasm / Q000235:genetics; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D006801:Humans; D009154:Mutation; D009369:Neoplasms / Q000235:genetics* / Q000503:physiopathology; D020641:Polymorphism, Single Nucleotide; D011237:Predictive Value of Tests; D011487:Protein Conformation; D018570:Risk Assessment; D012680:Sensitivity and Specificity
D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins