NTPDase and 5'-nucleotidase activities in rats with alloxan-induced diabetes.
Diabetes is associated with a hypercoagulable state. In this study, we investigated the potential effects of alloxan-induced diabetes on the activities of the enzymes NTPDase (E.C. 3.6.1.5, apyrase, ATP diphosphohydrolase, ecto/CD39) and 5'-nucleotidase (E.C. 3.1.3.5, CD73) that can control the levels of ADP and adenosine, two substances that regulates platelet aggregation. In the alloxan-treated rats, NTPDase activity was significantly increased by 88 and 35% with ATP as substrate and by 156 and 58% with ADP as substrate in platelets and synaptosomes, respectively (P< 0.05). AMP hydrolysis was increased by 142% (platelets) and 70% (synaptosomes) in diabetic rats compared to control. These results demonstrate that alloxan-induced diabetes interferes with ATP, ADP, and AMP hydrolysis in platelets and synaptosomes. Taken together, these results may indicate that in diabetic rats both NTPDase and 5'-nuleotidase from the central nervous system (CNS) and platelets respond similarly with increased activity. Thus, we speculate that platelets could be used as a potential peripheral marker of central alterations in NTPDase and 5'-nucleotidase activities in diabetes.
Lunkes
Gilberto I L
GI
Departamento de Ciências da Saúde, Universidade de Cruz Alta, Cruz Alta, RS, Brazil.
Lunkes
Daniele S
DS
Morsch
Vera M
VM
Mazzanti
Cintia M
CM
Morsch
André L B
AL
Miron
Vinícius R
VR
Schetinger
Maria R C
MR
D015720:5'-Nucleotidase / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D015703:Antigens, CD; D001081:Apyrase / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism*; D002540:Cerebral Cortex / Q000166:cytology / Q000201:enzymology; D002784:Cholesterol / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000201:enzymology*; D008297:Male; D010974:Platelet Aggregation / Q000502:physiology; D011897:Random Allocation; D051381:Rats; D013574:Synaptosomes / Q000201:enzymology; D014280:Triglycerides / Q000097:blood
D015703:Antigens, CD; D014280:Triglycerides; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D002784:Cholesterol; D015720:5'-Nucleotidase; D001081:Apyrase; C067869:CD39 antigen