Development of in vivo derived diploid and tetraploid pig embryos in a modified medium NCSU 37.
The aim of this study was to assess development of diploid and tetraploid in vivo derived pig embryos cultured in a modified medium NCSU 37 in an atmosphere with reduced concentration of oxygen. The tetraploid embryos were produced by electrofusion of two-cell embryos that had been cultured in vitro from the one-cell stage before fusion (cultured two-cell embryos) or by fusion of freshly recovered two-cell embryos. Development to blastocyst stage of tetraploid embryos, generated from the cultured two-cell embryos was significantly inferior to the development of control one-cell embryos (29.1 +/- 9.7% versus 66.8 +/- 9.7%; P < 0.05). However, development of tetraploid embryos produced from the freshly recovered two-cell embryos and control two-cell embryos was very similar (89.9 +/- 6.1% versus 81.3 +/- 3.4%). Detection of chromosomes 1 and 10 by in situ hybridization showed that more than 85% of the cultured control embryos were diploid while 15% of the embryos were mosaic. Among the fused embryos 50% were tetraploid, 29% mosaic and 21% diploid. These data indicate that the modified medium NCSU 37 provides optimum environment for pre-implantation development of pig diploid and tetraploid embryos.
Procházka
R
R
Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, 277 21 Libĕchov, Czech Republic. prochazka@iapg.cas.cz
Vodicka
P
P
Zudová
D
D
Rybár
R
R
Motlík
J
J
D000818:Animals; D002459:Cell Fusion; D003470:Culture Media; D046508:Culture Techniques; D004171:Diploidy; D004622:Embryo, Mammalian / Q000648:ultrastructure*; D005314:Embryonic and Fetal Development; D009030:Mosaicism; D010100:Oxygen / Q000008:administration & dosage; D011123:Polyploidy; D013552:Swine / Q000196:embryology*
D003470:Culture Media; D010100:Oxygen