Faster cyclic loess: normalizing RNA arrays via linear models.
BACKGROUND
Our goal was to develop a normalization technique that yields results similar to cyclic loess normalization and with speed comparable to quantile normalization.


RESULTS
Fastlo yields normalized values similar to cyclic loess and quantile normalization and is fast; it is at least an order of magnitude faster than cyclic loess and approaches the speed of quantile normalization. Furthermore, fastlo is more versatile than both cyclic loess and quantile normalization because it is model-based.


BACKGROUND
The Splus/R function for fastlo normalization is available from the authors.
Ballman
Karla V
KV
Division of Biostatistics, Mayo Clinic College of Medicine, Rochester, MN 55905, USA. ballman@mayo.edu
Grill
Diane E
DE
Oberg
Ann L
AL
Therneau
Terry M
TM
D000465:Algorithms; D016477:Artifacts; D020869:Gene Expression Profiling / Q000379:methods* / Q000592:standards; D014644:Genetic Variation; D016014:Linear Models; D008957:Models, Genetic; D009716:Numerical Analysis, Computer-Assisted; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods* / Q000592:standards; D012313:RNA / Q000235:genetics*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017423:Sequence Analysis, RNA / Q000379:methods* / Q000592:standards
D012313:RNA