title: [Involvement of TRAIL up-regulation of CD4+, CD8+ T cells in liver injury in chronic hepatitis B].
abstract: OBJECTIVE
  To describe a novel mechanism for TRAIL up-regulation of CD4+, CD8+ T cells to participate in the pathophysiological process in patients with chronic hepatitis B (CHB).
  
  
  METHODS
  The serum levels of soluble TRAIL (sTRAIL), IFN-gamma and membrane bound TRAIL expression on peripheral leucocytes from 58 CHB patients were examined by ELISA and flow cytometry respectively. The levels of TRAIL were compared with the baseline levels of 15 healthy controls, and correlation analysis were performed between ALT, TBil and PT, morphological change in hepatic tissues.
  
  
  RESULTS
  The results showed that TRAIL levels on membranes of CD4+, CD8+ T cells in CHB patients were much higher than the healthy controls (P < 0.001), which of CD4+ T cells positively correlated with serum TBil (r=0.354, P = 0.008), Serum IFN-gamma level (r=0.302, P = 0.011) and which of CD8+ T cells positively correlated with serum TBil (r=0.522, P = 0.000), ALT (r=0.393, P = 0.003), PT (r=0.385, P = 0.004), serum IFN-gamma level (r=0.307, P = 0.009). The serum levels of soluble TRAIL only correlated with serum HBeAg expression (r=0.695, P = 0.001).
  
  
  CONCLUSIONS
  These findings suggest that the expression of TRAIL on the membranes of lymphocytes was up-regulated, which may take part in the immunopathogenesis in CHB patients. TRAIL expression can be induced either by virus-specific protein expression or by inflammation cytokine IFN-gamma
authors.affiliation: Institute of Infectious Disease, the First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China.
authors.forename: Gong-ying
authors.initials: GY
authors.lastname: Chen
authors.forename: Jian-qin
authors.initials: JQ
authors.lastname: He
authors.forename: Guo-cai
authors.initials: GC
authors.lastname: Lv
authors.forename: Min-wei
authors.initials: MW
authors.lastname: Li
authors.forename: Chen-huai
authors.initials: CH
authors.lastname: Xu
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Fan
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D051017:Apoptosis Regulatory Proteins; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry*; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry*; D004279:DNA, Viral / Q000097:blood; D005260:Female; D019694:Hepatitis B, Chronic / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000097:blood*; D008875:Middle Aged; D053221:TNF-Related Apoptosis-Inducing Ligand; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D015854:Up-Regulation
chemical_list: D051017:Apoptosis Regulatory Proteins; D004279:DNA, Viral; D008562:Membrane Glycoproteins; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma