title: [Effect of antisense CD40 RNA on CD40 expression and proliferation of EB virus-transformed B lymphocytes].
abstract: OBJECTIVE
  To investigate the changes of CD40 expression and proliferation pattern of EB virus-transformed B lymphocytes that had been transfected by antisense CD40 RNA.
  
  
  METHODS
  Eukaryotic expression vector pcDNA3/CD40 of human CD40antisense RNA was constructed by means of T-A cloning and subcloning, and then was transfected into EB virus-transformed B lymphocytes. Changes of CD40 expression on the B cells and their proliferation were tested by flow cytometry and MTT colorimetry assay, respectively.
  
  
  RESULTS
  CD40 expression and proliferation of the B cells transfected with antisense CD40 RNA were significantly lower than those of the cells transfected with pcDNA3 alone (P < 0. 01).
  
  
  CONCLUSIONS
  Antisense CD40 RNA may be served as an effective means of regulating immune function, and the CD40 gene itself also play an important role in cell proliferation.
authors.affiliation: Institute of Clinical Immunology, Union Hospital Affiliated to Fujian Medical University, Fuzhou 350001, China. Zhengx@pub1.fz.fj.cn
authors.forename: Xiang-xiong
authors.initials: XX
authors.lastname: Zheng
authors.forename: He-jun
authors.initials: HJ
authors.lastname: Li
authors.forename: Pin
authors.initials: P
authors.lastname: Li
authors.forename: Xiao-ling
authors.initials: XL
authors.lastname: Zhou
mesh_terms: D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000821:virology; D019013:CD40 Antigens / Q000235:genetics*; D002461:Cell Line, Transformed; D004854:Herpesvirus 4, Human; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D016372:RNA, Antisense / Q000494:pharmacology*
chemical_list: D019013:CD40 Antigens; D016372:RNA, Antisense