B-cell development and pre-B-1 cell plasticity in vitro.
B-lymphopoiesis in vivo is a very complex process that is influenced by cooperation between cells, cytokines, and other receptor-ligand interactions, which developmentally occur at different cellular stages. Various in vitro models have been very useful in unraveling this complex process of B-cell development. Here the protocols for how to grow and to study the differentiation of mouse pre-B-1, as developed in our laboratory, are described. Moreover, the protocols of how to grow and to test the plasticity of Pax-5-deficient pre-B-1 cells are also outlined.
Rolink
Antonius G
AG
Division of Molecular Immunology, Pharmazentrum, University of Basel, Basel, Switzerland.
D000818:Animals; D015703:Antigens, CD / Q000276:immunology / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000378:metabolism; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology / Q000494:pharmacology*; D038041:Lymphopoiesis / Q000276:immunology*; D051379:Mice; D018448:Models, Immunological; D051757:PAX5 Transcription Factor; D012190:Retroviridae / Q000235:genetics / Q000502:physiology; D017154:Stromal Cells / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D019065:Virus Assembly / Q000235:genetics / Q000502:physiology
D015703:Antigens, CD; D004268:DNA-Binding Proteins; D007075:Immunoglobulin M; D051757:PAX5 Transcription Factor; C497282:Pax5 protein, mouse; D014157:Transcription Factors