Transcription coactivator PBP, the peroxisome proliferator-activated receptor (PPAR)-binding protein, is required for PPARalpha-regulated gene expression in liver.
Nuclear receptor coactivator PBP (peroxisome proliferator-activated receptor (PPAR)-binding protein) functions as a coactivator for PPARs and other nuclear receptors. PBP serves as an anchor for TRAP (thyroid hormone receptor-associated proteins)/mediator multisubunit cofactor transcription complex. Disruption of the PBP/TRAP220 gene results in embryonic lethality around embryonic day 11.5 by affecting placental, cardiac, hepatic, and bone marrow development. Because PPAR isoforms alpha, gamma, and beta/delta function as important regulators of lipid homeostasis in mammals, it becomes important to assess the requirement of coactivator PBP in the regulation of PPAR functions in vivo. Sustained activation of PPARalpha by structurally diverse classes of chemicals of biological importance, designated peroxisome proliferators, leads to proliferation of peroxisomes in liver, induction of PPARalpha target genes including those involved in fatty acid oxidation, and the eventual development of liver tumors. Here, we show that targeted deletion of PBP in liver parenchymal cells, using the Cre-loxP system, results in the near abrogation of PPARalpha ligand-induced peroxisome proliferation and liver cell proliferation, as well as the induction of PPARalpha-regulated genes in PBP-deficient liver cells. In contrast, scattered PBP(+/+) hepatocytes in these livers showed DNA synthesis and were markedly hypertrophic with peroxisome proliferation in response to PPARalpha ligands. Chromatin immunoprecipitation data suggest that in PBP conditional null livers, there appears to be reduced association of cofactors, especially of CBP and TRAP150, to the mouse enoyl-CoA hydratase/l-3-hydroxyacyl-CoA dehydrogenase gene promoter. These observations suggest that PBP is required for the stabilization of multiprotein cofactor complexes. In essence, the absence of PBP in hepatocytes in vivo appears to mimic the absence of PPARalpha, indicating that coactivator PBP is essential for PPARalpha-regulated gene expression in liver parenchymal cells.
Jia
Yuzhi
Y
Department of Pathology, Northwestern University, Feinberg School of Medicine, Illinois 60611-3008, USA.
Qi
Chao
C
Kashireddi
Papreddy
P
Surapureddi
Sailesh
S
Zhu
Yi-Jun
YJ
Rao
M Sambasiva
MS
Le Roith
Derek
D
Chambon
Pierre
P
Gonzalez
Frank J
FJ
Reddy
Janardan K
JK
D000256:Adenoviridae / Q000235:genetics; D000483:Alleles; D000821:Animal Feed; D000818:Animals; D015152:Blotting, Northern; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004583:Electrons; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D017353:Gene Deletion; D005786:Gene Expression Regulation; D005838:Genotype; D022781:Hepatocytes / Q000378:metabolism; D015151:Immunoblotting; D007150:Immunohistochemistry; D008024:Ligands; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism*; D056913:Mediator Complex Subunit 1; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008957:Models, Genetic; D010100:Oxygen / Q000378:metabolism; D020025:Peroxisome Proliferators / Q000494:pharmacology; D011233:Precipitin Tests; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011743:Pyrimidines / Q000494:pharmacology; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D011995:Recombination, Genetic; D013997:Time Factors; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology*
D002843:Chromatin; D005227:Fatty Acids; D008024:Ligands; C538856:Med1 protein, mouse; D056913:Mediator Complex Subunit 1; D020025:Peroxisome Proliferators; D011743:Pyrimidines; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; C006253:pirinixic acid; D004247:DNA; D010100:Oxygen