Expression of cytochrome P4502E1 gene in hepatocellular carcinoma.
OBJECTIVE
To investigate cytochrome P4502E1 (CYP2E1) gene expression in occurrence and progression of hepatocellular carcinoma (HCC).


METHODS
The human liver arrayed library was spotted onto the nylon membranes to make cDNA array. Hybridization of cDNA array was performed with labeled probes synthesized from RNA isolated from HCC and adjacent liver tissues. Sprague-Dawley rats were administrated diethylnitrosamine (DENA) to induce HCC. CYP2E1 expression was detected by the method of RT-PCR and Northern blot analysis.


RESULTS
CYP2E1 was found by cDNA array hybridization to express differently between HCC and liver tissues. CYP2E1 only expressed in liver, but did not express in HCC tissues and expressed lowly in cirrhotic tissues. In the progression of cirrhosis and HCC, the expression level of CYP2E1 was gradually decreased and hardly detected until the late stage of HCC.


CONCLUSIONS
Using arrayed library to make cDNA arrays is an effective method to find differential expression genes. CYP2E1 is a unique gene expressing in liver but did not express in HCC. CYP2E1 expression descended along with the initiation and progression of HCC, which is noteworthy further investigations in its significance in the development of HCC.
Man
Xiao-Bo
XB
International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Hospital, 225 Changhai Road, Shanghai 200438, China.
Tang
Liang
L
Qiu
Xiu-Hua
XH
Yang
Li-Qun
LQ
Cao
Hui-Fang
HF
Wu
Meng-Chao
MC
Wang
Hong-Yang
HY
D000818:Animals; D015152:Blotting, Northern; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced / Q000235:genetics*; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D006801:Humans; D008113:Liver Neoplasms / Q000139:chemically induced / Q000235:genetics*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
D019392:Cytochrome P-450 CYP2E1