Monitoring the self-assembly of chitosan/glutaraldehyde/cysteamine/Au-colloid and the binding of human serum albumin with hesperidin.
A new method for monitoring, in real-time, the self-assembly of chitosan/glutaraldehyde/cysteamine (CGC) on the gold surface and the immobilization of Au-colloid on CGC membrane with piezoelectric quartz crystal impedance (PQCI) are firstly proposed. Cyclic voltammogram and electrochemical impedance spectroscopy were also use to investigate the formation of Au-colloid/CGC. The viscosity-average molecular weight of chitosan used was firstly estimated as 16.8 x 10(5) with piezoelectric quartz crystal (PQC) sensor. On the basis of the analysis of the multi-dimensional information provided by PQCI, two stages existed in chitosan drying course: the frequency shift of the first stage was controlled by viscoelastic of the liquid, while the total frequency shift was due to mass change. The cross-link ratio of glutaraldehyde with chitosan was about 0.13, while for glutaraldehyde with cysteamine was about 0.217. PQCI also showed that the Au-colloid immobilization is a first-order reaction, while the HSA immobilization is a sum of two exponential functions, e.g., adsorption and re-arrangement. The association of the immobilized HSA with the purified hesperidin was monitored, and the association constant was estimated as 3.42 ml mg(-1) by Scatchard analysis.
Liu
Yingju
Y
College of Chemistry and Chemical Engineering, Hunan University, State Key Laboratory of Chemo/Biosensing and Chemometrics, Changsha, Hunan 410082, China.
Li
Yanling
Y
Liu
Shanchao
S
Li
Jia
J
Yao
Shouzhuo
S
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D048271:Chitosan / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003460:Crystallization / Q000379:methods*; D003543:Cysteamine / Q000737:chemistry; D017097:Electric Impedance; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005976:Glutaral / Q000737:chemistry; D017979:Gold Colloid / Q000032:analysis / Q000737:chemistry*; D006569:Hesperidin / Q000737:chemistry*; D006801:Humans; D009862:Online Systems; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D017979:Gold Colloid; D012709:Serum Albumin; D003543:Cysteamine; D048271:Chitosan; D006569:Hesperidin; D005976:Glutaral