Arthrobacter nitroguajacolicus sp. nov., a novel 4-nitroguaiacol-degrading actinobacterium.
Three bacterial isolates from soil, capable of degradation or transformation of nitroaromatic compounds and displaying a rod-coccus growth cycle, were studied by a polyphasic approach. On the basis of 16S rRNA sequence analysis and of chemotaxonomic characteristics, such as type A3alpha peptidoglycan with an interpeptide bridge Ala-Thr-Ala, the major menaquinone MK-9(H(2)) and fatty acid composition, the isolates were assigned to the genus Arthrobacter. DNA-DNA hybridization, riboprinting and phenotypic studies revealed that the three strains constitute a single species, distinct from phylogenetically neighbouring Arthrobacter aurescens and Arthrobacter ilicis. A novel species, Arthrobacter nitroguajacolicus sp. nov., with the type strain G2-1(T) (=CCM 4924(T)=DSM 15232(T)) is proposed.
Kotoučková
Ludmila
L
Department of Microbiology, Faculty of Science, Masaryk University Brno, Tvrdého 14, 602 00 Brno, Czech Republic.
Schumann
Peter
P
DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
Durnová
Eva
E
Regional Institute of Public Health, Ostrava, Czech Republic.
Spröer
Cathrin
C
DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
Sedláček
Ivo
I
CCM - Czech Collection of Microorganisms, Masaryk University Brno, Czech Republic.
Neča
Jiří
J
Veterinary Research Institute, Brno, Czech Republic.
Zdráhal
Zbyněk
Z
Laboratory of Functional Genomics and Proteomics, Faculty of Science, Masaryk University Brno, Czech Republic.|Institute of Analytical Chemistry, Brno, Czech Republic.
Němec
Miroslav
M
Department of Microbiology, Faculty of Science, Masaryk University Brno, Tvrdého 14, 602 00 Brno, Czech Republic.
D000595:Amino Acid Sequence; D001173:Arthrobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001482:Base Composition; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006139:Guaiacol / Q000031:analogs & derivatives / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000235:genetics; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D006139:Guaiacol