Bioimaging TOF-SIMS: localization of cholesterol in rat kidney sections.
Here, we show the localization of a whole organic molecule in biological tissue using time-of-flight secondary ion mass spectrometry (TOF-SIMS). Rat kidneys were sectioned by cryoultramicrotomy and dried at room temperature. The samples were covered with a thin silver layer and analyzed in an imaging TOF-SIMS instrument equipped with a Ga(+)-source. The cholesterol signal showed a high concentration in the nuclear areas of the epithelial cells and a lower concentration over areas representing the basal lamina of renal tubules. A more diffuse distribution of cholesterol was also found over areas representing the cytoplasm or plasma membrane of the epithelial cells.
Nygren
Håkan
H
Department of Anatomy and Cell Biology, University of Göteborg, P.O. Box 420, SE 405 30 Göteborg, Sweden. hakan.nygren@anatcell.gu.se
Malmberg
Per
P
Kriegeskotte
Christian
C
Arlinghaus
Heinrich F
HF
D000818:Animals; D002784:Cholesterol / Q000737:chemistry / Q000378:metabolism*; D016616:Cryoultramicrotomy; D007091:Image Processing, Computer-Assisted; D007668:Kidney / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008853:Microscopy / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D012834:Silver / Q000737:chemistry; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods*
D012834:Silver; D002784:Cholesterol