An immunohistochemical study of endocrine cells in the alimentary tract of the grass lizard, Takydromus wolteri Fischer (Laceridae).
Distribution patterns and the relative frequency of different types of endocrine cells were demonstrated in the alimentary tract of the grass lizard, Takydromus wolteri, using nine specific antibodies raised against mammalian regulatory peptides. The alimentary tract of the lizard was divided into six portions from the esophagus to the rectum. Most endocrine cells were found in the epithelial lining and were generally spindle shaped with long cytoplasmic processes ending in the lumen (open cell type), whereas cells that were spherical in shape (closed cell type) were occasionally found in gastric, esophageal and intestinal glands. Endocrine cells were stained for the following regulatory peptides: bovine Sp-1/chromogranin (BCG), serotonin, somatostatin, gastrin, cholecystokinin (CCK)-8, glucagon, insulin, human pancreatic polypeptide (HPP) and secretin. Cells stained for BCG and serotonin were present throughout the entire gastrointestinal tract and they occurred with the highest frequency in stomach and pylorus, respectively. Somatostatin-positive cells were detected throughout the entire gastrointestinal tract except for the esophagus and large intestine, and were most predominant in pylorus and duodenum. Cells stained for gastrin were restricted to the pylorus and duodenum and occurred with a relatively low frequency. CCK-8-positive cells were observed from pylorus to small intestine and showed the highest frequency in the pylorus. Glucagon- and insulin-containing cells were located in duodenum and small intestine but were found only rarely. HPP-stained cells were detected in duodenum and small intestine with the highest frequency in duodenum. Cells stained for secretin were restricted to duodenum and were found only rarely. In conclusion, distribution patterns and the relative frequency of these endocrine cells correspond well with previous reports on distribution patterns of endocrine cells in reptile species but some deviating patterns were also observed.
Lee
Hyeung Sik
HS
Department of Herbal Biotechnology, Daegu Haany University, Gyeongsan, Gyeongbuk, 712-715, Republic of Korea. endohist@dhu.ac.kr
Ku
Sae Kwang
SK
D000818:Animals; D002766:Cholecystokinin / Q000096:biosynthesis*; D002864:Chromogranins / Q000096:biosynthesis*; D019858:Enteroendocrine Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D041981:Gastrointestinal Tract / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D008116:Lizards / Q000033:anatomy & histology / Q000378:metabolism*; D008297:Male; D036361:Peptide Hormones / Q000096:biosynthesis*; D012701:Serotonin / Q000096:biosynthesis*
D002864:Chromogranins; D036361:Peptide Hormones; D012701:Serotonin; D002766:Cholecystokinin