[The effect of artificial support system on serum cytokine in chronic severe hepatitis].
OBJECTIVE
To explore the changes of cytokines including TNFalpha, TGFbeta1 and nitrogen monoxide, and endotoxin in the serum of chronic severe hepatitis after the treatment of ALSS, and to evaluate further the value of ALSS in the treatment of chronic severe hepatitis.


METHODS
Forty two patients were screened. The changes of TNFalpha, TGFbeta1, nitrogen monoxide and endotoxin were detected respectively. The relationship between the cytokines and the severity and prognosis were further analyzed.


RESULTS
ALSS was effective to decrease the serum concentration of cytokines. TNFalpha dropped from (481.57+/-229.33) pg/ml to (156.46+/-78.12) pg/ml (P < 0.05). TGFbeta1 from (44.09+/-31.73) ng/ml to (27.77+/-23.28) ng/ml (P < 0.01), endotoxin from (1.05+/-0.37) Eu/ml to (0.28+/-0.22) Eu/ml (P < 0.001). NO from (71.15+/-33.09) micromol/L to (58.11+/-29.30) micromol/L (P < 0.001). Before the therapy endotoxin was related with TNFalpha and total bilirubin, while after the therapy, NO was related with protime and aminonemia.


CONCLUSIONS
High level of endotoxin and nitrogen monoxide in serum plays an important role in hepatocyte damage of chronic severe hepatitis. The changes of serum endotoxin TNFalpha, TGFbeta1 and nitrogen monoxide level in patients with chronic severe hepatitis can be used to judge the severity and prognosis of severe hepatitis. ALSS is a reliable hepatic support device for chronic severe hepatitis
Qin
Bo
B
Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
Qie
Chun-hua
CH
Zhang
Da-zhi
DZ
Zhao
You-rong
YR
Guo
Shu-hua
SH
Wang
Zhi-yi
ZY
Zhou
Zhi
Z
D000328:Adult; D000368:Aged; D016207:Cytokines / Q000097:blood*; D004731:Endotoxins / Q000097:blood; D005260:Female; D006521:Hepatitis, Chronic / Q000097:blood / Q000276:immunology / Q000628:therapy*; D006801:Humans; D019164:Liver, Artificial; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood; D011379:Prognosis; D016212:Transforming Growth Factor beta / Q000097:blood
D016207:Cytokines; D004731:Endotoxins; D016212:Transforming Growth Factor beta; D009569:Nitric Oxide