The effect of high local concentrations of antibiotics on demineralized bone induction in rats.
OBJECTIVE
This study evaluated the effect of high local concentrations of antibiotics on bone repair induced by demineralized bone in rats.


METHODS
Seventy-two 3-week-old rats, weighing 200 to 300 g, were used for the experiment. Rats were divided into 4 groups: control group (group 1), saline-impregnation group (group 2), gentamicin-impregnation group (group 3), and tetracycline-impregnation group (group 4). The rats were killed at 3, 8, and 12 weeks after surgery. Samples were stained with hematoxylin-eosin and subjected to the histomorphometric analyses.


RESULTS
There were significant differences in new bone formation among groups at all time periods. Comparing differences between groups at each time point, significantly more new bone formation was present in group 2 than in group 1 at 3 weeks, more in group 2 than in group 1 and more in group 2 than in group 4 at 8 weeks, and the most in group 2 among all groups at 12 weeks. In terms of the time period, significantly more new bone formation was observed at 8 weeks during the time period between 3 and 8 weeks, and at 12 weeks during the time period between 3 and 12 weeks.


CONCLUSIONS
Our result suggests that the bone graft material is most effective when mixed with saline for the regeneration of osseous defects.
Kim
Su-Gwan
SG
Department of Oral and Maxillofacial Surgery, College of Dentistry, Chosun University, Gwang-Ju, Korea. SGCKIM@mail.chosun.ac.kr
Chung
Tae-Young
TY
Kim
Myong Soo
MS
Lim
Sung-Chul
SC
D000704:Analysis of Variance; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D016025:Bone Transplantation / Q000379:methods / Q000502:physiology*; D005839:Gentamicins / Q000494:pharmacology*; D006801:Humans; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D012886:Skull / Q000601:surgery; D013752:Tetracycline / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D013752:Tetracycline