[Construction and Identification of DNA vaccine containing chimeric gene gag-gp120 of HIV-1].
OBJECTIVE
To construct DNA vaccine expression plasmid containing the chimeric gene gag-gp120 of HIV-1.


METHODS
The recombinant eukaryotic expression vector pVAXGE was constructed via inserting the chimeric gene gag-gp120 into the vector pVAX1. Hela cells had been transfected by recombinant plasmid via liposome. After 72 h, the transfected cells was detected by RT-PCR and analyzed by Dot-ELISA.


RESULTS
The transcript products of target gene could be amplified from the cells transfected by recombinant plasmid. Dot-ELISA detection showed that the target gene was expressed in Hela cells.


CONCLUSIONS
The DNA vaccine plasmid expressing chimeric gene gag-gp120 was successfully constructed, which lays the foundation for preparing DNA vaccine against HIV-1.
Jiang
Wen-Zheng
WZ
Key Laboratory of Gene Engineering of PLA, Quartermaster University of PLA, Changchun 130062,China.
Jin
Ning-Yi
NY
Han
Wen-Yu
WY
D005822:Genetic Vectors; D015699:HIV Envelope Protein gp120; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D010957:Plasmids; D019444:Vaccines, DNA / Q000276:immunology
D015699:HIV Envelope Protein gp120; D019444:Vaccines, DNA