Very high pressure HPLC with 1 mm id columns.
Theoretical calculations and experimental data indicate that very high pressure HPLC can be performed using 1 and 1.5 mm id columns, and contrary to previous beliefs, the frictional heating generated does not appear to be detrimental to the separation.
Colón
Luis A
LA
Department of Chemistry, University at Buffalo, The State University of New York, NS Complex, Buffalo, New York 14260-3000, USA. lacolon@buffalo.edu
Cintrón
José M
JM
Anspach
Jason A
JA
Fermier
Adam M
AM
Swinney
Kelly A
KA
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D006358:Hot Temperature