title: Galactosylceramide expression factor-1 induces myogenesis in MDCK and C3H10T1/2 cells.
abstract: We previously reported that galactosylceramide expression factor-1 (GEF-1), a rat homolog of hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs/Hgs), induces galactosylceramide and/or sulfatide expression and morphological changes in epithelial cells. Here, we show that GEF-1 induces myogenesis in MDCK and C3H10T1/2 cells. GEF-1 overexpression in MDCK cells (MDCK/GEF-1) appeared to promote trans-differentiation to myoblasts that expressed MyoD and myosin heavy chain (MHC). MDCK/GEF-1 cells also expressed several DNA-binding proteins (MyoD and MEF-2) that are essential for myogenesis. These results suggest that GEF-1 induces MDCK cells to enter an early stage of myogenesis. Subsequently, we tested whether GEF-1 could induce myogenesis in C3H10T1/2 mouse fibroblasts, which have the potential to differentiate into myoblast-like cells. Indeed, GEF-1 induced morphological changes that were consistent with myoblast-like cells, and both MyoD and MHC were expressed. Our results suggest that GEF-1 may induce MDCK and C3H10T1/2 cells to trans-differentiate into myoblast-like cells.
authors.affiliation: Department of Tumor Immunology, Tokyo Metropolitan Institute of Medical Science, The Tokyo Metropolitan Organization for Medical Research, 3-18-22, Honkomagome, Bunkyo-Ku, Tokyo 113-8613, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ogura
authors.forename: Yuko S
authors.initials: YS
authors.lastname: Niino
authors.forename: Tadashi
authors.initials: T
authors.lastname: Tai
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism; D004285:Dogs; D056827:Endosomal Sorting Complexes Required for Transport; D004848:Epithelium / Q000378:metabolism* / Q000648:ultrastructure*; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism*; D007668:Kidney; D064326:MEF2 Transcription Factors; D051379:Mice; D008809:Mice, Inbred C3H; D017570:MyoD Protein / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology* / Q000502:physiology*; D018006:Myogenic Regulatory Factors; D018995:Myosin Heavy Chains / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D064326:MEF2 Transcription Factors; D017570:MyoD Protein; D018006:Myogenic Regulatory Factors; D010750:Phosphoproteins; D011994:Recombinant Proteins; D014157:Transcription Factors; D000251:Adenosine Triphosphatases; C115505:Hgs protein, rat; D018995:Myosin Heavy Chains