title: Essential role of the interstitial cells of Cajal in nitric oxide-mediated relaxation of longitudinal muscle of the mouse ileum.
abstract: The role of interstitial cells of Cajal (ICC) in electrical field stimulation (EFS)-induced neurogenic responses in ileum was studied by using the ICC-deficient mutant (SLC-W/W(V)) mouse and its wild type. In the immunohistochemical study with anti-c-Kit antibody, ICC was observed in the myenteric plexus (MY) and deep muscular plexus (DMP) region in the wild type. In the mutant, ICC-MY were lost, only ICC-DMP were present. EFS induced a rapid contraction of the ileal segments from the wild type mouse in the direction of longitudinal muscle. In the mutant mouse, onset of contraction was delayed and its rate was slowed. EFS induced nonadrenergic, noncholinergic (NANC) relaxation in the presence of atropine and guanethidine in the wild type. A nitric oxide synthase inhibitor inhibited the relaxation and L-arginine reversed it. In the mutant, EFS did not induce NANC relaxation. There was no difference between the responsiveness of the segments from wild type and mutant mice to exogenously added acetylcholine or Nor-1. Taking into account the selective loss of ICC-MY in the mutant mice, it seems likely that ICC-MY have an essential role in inducing nitric oxide-mediated relaxation of longitudinal muscle of the mouse ileum and that ICC-MY partly participate in EFS-induced contraction.
authors.affiliation: Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, Osaka Prefecture University, Sakai, Japan. takeuchi@vet.osakafu-u.ac.jp
authors.forename: Tadayoshi
authors.initials: T
authors.lastname: Takeuchi
authors.forename: Kaori
authors.initials: K
authors.lastname: Fujinami
authors.forename: Akikazu
authors.initials: A
authors.lastname: Fujita
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Okishio
authors.forename: Tadashi
authors.initials: T
authors.lastname: Takewaki
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Hata
mesh_terms: D000818:Animals; D003239:Connective Tissue Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D007082:Ileum / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008817:Mice, Mutant Strains; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D009126:Muscle Relaxation / Q000187:drug effects / Q000502:physiology*; D009569:Nitric Oxide / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000494:pharmacology
chemical_list: D020030:Nitric Oxide Donors; D009569:Nitric Oxide