title: Relationship between clinical and environmental isolates of Pseudomonas aeruginosa in a hospital setting.
abstract: BACKGROUND
  Populations of Pseudomonas aeruginosa have been extensively studied, although there is no general agreement concerning their genetic structure. It has been proposed that P. aeruginosa is a very homogeneous species with 90% of individuals within the same clonal group; nonetheless, other results suggested that Pseudomonas populations are panmictic. Here we compared P. aeruginosa populations from clinical and environmental samples, both isolated from the Bellvitge Hospital of the University of Barcelona in Spain.
  
  
  METHODS
  Antibiotic susceptibility determination as well as whole cell and outer membrane protein denaturing gel electrophoresis, pulsed-field electrophoresis, and random amplified polymorphic DNA analysis were performed.
  
  
  RESULTS
  Environmental isolates were much more susceptible to antibiotics than those isolated from clinical specimens. The remainder of the analyses revealed high degree of diversity.
  
  
  CONCLUSIONS
  Whole-cell proteins, outer-membrane proteins, and pulsed field electrophoresis did not support a close relationship between clinical and environmental isolates. Random amplified polymorphic DNA (RAPD) confirmed the distance between isolates from both sources. This suggests that the origin of hospital infections by P. aeruginosa is due mainly to growth of bacterial strains acquired by patients prior to hospital admission or from patient-to-patient through healthcare workers (HCWs).
authors.affiliation: Unitat de Microbiologia, Servei de Microbiologia, Hospital de Bellvitge, Universitat de Barcelona, Hospitalet de Llobregat, Spain.
authors.forename: Lídia
authors.initials: L
authors.lastname: Ruiz
authors.forename: M Angeles
authors.initials: MA
authors.lastname: Domínguez
authors.forename: Neus
authors.initials: N
authors.lastname: Ruiz
authors.forename: Miguel
authors.initials: M
authors.lastname: Viñas
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D003428:Cross Infection; D004352:Drug Resistance, Microbial; D016521:Electrophoresis, Gel, Pulsed-Field; D004591:Electrophoresis, Polyacrylamide Gel; D005838:Genotype; D006761:Hospitals; D006801:Humans; D011110:Polymorphism, Genetic; D011552:Pseudomonas Infections / Q000175:diagnosis*; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D000900:Anti-Bacterial Agents