title: Microfluidic device reads up to four consecutive base pairs in DNA sequencing-by-synthesis.
abstract: We have developed the first fully integrated microfluidic system for DNA sequencing-by-synthesis. Using this chip and fluorescence detection, we have reliably sequenced up to 4 consecutive bps. The described sequencer can be integrated with other microfluidic components on the same chip to produce true lab-on-a-chip technology. The surface chemistry that was designed to anchor the DNA to elastomeric microchannels is useful in a broad range of studies and applications.
authors.affiliation: Applied Physics Department, California Institute of Technology, Pasadena, CA 91125, USA.
authors.forename: Emil P
authors.initials: EP
authors.lastname: Kartalov
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Quake
mesh_terms: D020029:Base Pairing; D001483:Base Sequence; D004247:DNA / Q000096:biosynthesis / Q000737:chemistry; D044085:Microfluidics / Q000295:instrumentation*; D017422:Sequence Analysis, DNA / Q000295:instrumentation* / Q000379:methods
chemical_list: D004247:DNA