title: Transcriptional analysis of the groE and dnaK heat-shock operons of Enterococcus faecalis.
abstract: Enterococcus faecalis is able to survive in extremely adverse conditions, and its ability to resist stress is considered a key virulence attribute. Here, we conducted a detailed transcriptional analysis of the groE and dnaK operons of E. faecalis. The dnaK operon is comprised of four genes (hrcA-grpE-dnaK-dnaJ) preceded by two conserved CIRCE sequences. The dnaK operon is expressed from a sigmaA-type promoter located upstream of hrcA and multiple transcripts are detectable, possibly due to mRNA processing. The groE operon (groES-groEL) is transcribed as a single mRNA from a sigmaA-type promoter located immediately upstream of a CIRCE element. Induction of dnaK and groEL occurs in response to heat shock and exposure to NaCl, SDS and H(2)O(2).
authors.affiliation: Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Marinella S
authors.initials: MS
authors.lastname: Laport
authors.forename: José A C
authors.initials: JA
authors.lastname: Lemos
authors.forename: Maria do Carmo F
authors.initials: Mdo C
authors.lastname: Bastos Md
authors.forename: Robert A
authors.initials: RA
authors.lastname: Burne
authors.forename: Marcia
authors.initials: M
authors.lastname: Giambiagi-De Marval
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D018833:Chaperonins; D017124:Conserved Sequence; D004269:DNA, Bacterial / Q000032:analysis; D013293:Enterococcus faecalis / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012965:Sodium Chloride / Q000494:pharmacology; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C028715:GroE protein, E coli; D006360:Heat-Shock Proteins; D012965:Sodium Chloride; D018833:Chaperonins