title: [Construction and expression of retroviral vector containing bone morphogenetic protein-7].
abstract: OBJECTIVE
  To achieve stable and long-term expression of BMP-7 gene in bone marrow stem cells (BMSCs).
  
  
  METHODS
  Retrovirus expression vector containing BMP-7 gene was constructed and transfected into packaging cells PT67. After puromycin selection and cells cloning, cell clones producing high level of recombinant virus were obtained. The viruses were used to infect directly BMSCs and the expression of BMP-7 gene in BMSCs was analyzed by immunohistochemical staining.
  
  
  RESULTS
  Retrovirus vector containing BMP-7 gene was successfully reconstructed and BMP-7 was expressed in the BMSCs.The transfection rates was about 30%-40%.
  
  
  CONCLUSIONS
  The construction of recombinant retrovirus vector containing BMP-7 gene can provide a reliable tool for the formation of bone or cartilage seed cells in the research of tissue engineering.
authors.affiliation: Key Laboratory of Tissue Engineering, Department of Plastic & Reconstructive Surgery, Affiliated Ninth People Hospital, Shanghai Second Medical University, Shanghai 200011, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Zhu
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.forename: De-Li
authors.initials: DL
authors.lastname: Liu
authors.forename: Fang-Jun
authors.initials: FJ
authors.lastname: Liu
authors.forename: Juan-Juan
authors.initials: JJ
authors.lastname: Wu
authors.forename: Jun-Hong
authors.initials: JH
authors.lastname: Lu
authors.forename: Yi-Lin
authors.initials: YL
authors.lastname: Cao
mesh_terms: D001854:Bone Marrow Cells / Q000378:metabolism; D055419:Bone Morphogenetic Protein 7 / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D023822:Tissue Engineering; D014162:Transfection
chemical_list: D055419:Bone Morphogenetic Protein 7