Relevance of radioprotectors in radiotherapy: studies with tocopherol monoglucoside.
Radioprotective compounds are of importance in clinical radiation therapy, because normal tissues should be protected against radiation injury while using higher doses of radiation to obtain better cancer control. We investigated the radioprotection of cellular DNA in cancer and in various cells and tissues, in a murine system following exposure to gamma-radiation and tocopherol monoglucoside (TMG) administration. We used single-cell gel electrophoresis (comet assay) and studied the progression of murine fibrosarcoma following radiation exposure and administration of TMG. The administration of TMG to tumor-bearing mice protected the cellular DNA against radiation-induced strand breaks as shown by the decrease in comet tail length, tail moment, and percentage of DNA in the tails of the cells of normal tissues. The same parameters were not altered in the cells of fibrosarcoma. Our results showed that the administration of TMG immediately after exposure to gamma-radiation can protect normal tissues against radiation damages in tumor-bearing mice. Local gamma-radiation exposure (5 Gy) of the tumor retarded the tumor growth. Administration of TMG did not protect cancer cells from radiation damage because the growth curves of cancer cells treated with radiation alone and those treated with TMG after irradiation were not significantly different.
Nair
Cherupally Krishnan K
CK
Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Bombay, India. cknair@magnum.barc.ernet.in
Salvi
Veena
V
Kagiya
Tsutomu V
TV
Rajagopalan
Rema
R
D000818:Animals; D002460:Cell Line / Q000187:drug effects / Q000528:radiation effects; D045744:Cell Line, Tumor / Q000187:drug effects / Q000528:radiation effects; D005354:Fibrosarcoma / Q000517:prevention & control*; D005960:Glucosides / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009381:Neoplasms, Radiation-Induced / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D024505:Tocopherols / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology*
D005960:Glucosides; D011837:Radiation-Protective Agents; C460320:tocopherol monoglucoside; D024505:Tocopherols