[Study on the affinities of antagonistic peptides with HAb18G/CD147 on the hepatocellular carcinoma cells].
OBJECTIVE
To study the affinities of antagonistic peptides with HAb18G/CD147 on the hepatocellular carcinoma cells.


METHODS
Fluorescence-activated cell sorting analysis(FACS) was used to detect the expression of HAb18G antigen on HHCC and SMMC7721 cells. Antagonistic peptides(APs) AP-1, AP-2 and AP-6 were labelled with biotin at their N-terminals. The binding abilities of APs to HHCC or SMMC7721 cells were determined by FACS and confocal microscope.


RESULTS
HAb18G antigen was highly expressed on the HHCC and SMMC7721 cells. AP-6 has the highest affinity for HHCC and SMMC7721 cells compared with AP-1 and AP-2.


CONCLUSIONS
Antagonistic peptides of HAb18G/CD147 have high affinities with HAb18G/CD147 on the hepatocellular carcinoma cells.
Qian
Ai-Rong
AR
Cell Engineering Research Centre, Fourth Military Medical University, Xi'an 710032,China.
Shang
Peng
P
Li
Yu
Y
Fan
Chun-Mei
CM
Chen
Dan
D
Wang
Chun-Mei
CM
Zhu
Ping
P
Chen
Zhi-Nan
ZN
D051926:Basigin / Q000378:metabolism; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D045744:Cell Line, Tumor; D006801:Humans; D008113:Liver Neoplasms; D010455:Peptides
D010455:Peptides; D051926:Basigin