title: In-vitro maturation of germinal-vesicle oocytes and cryopreservation in metaphase I/II: a possible additional option to preserve fertility during ovarian tissue cryopreservation.
abstract: This study describes the possibility of combining two options in order to preserve female fertility: cryopreservation of human ovarian tissue and in-vitro matured germinal vesicle (GV) oocytes retrieved during tissue dissection. In contrast to ovarian tissue cryopreservation, the cryostorage of in-vitro matured unfertilized metaphaseI/II oocytes could be a more realistic option. This concept of preserving fertility before chemotherapy and/or radiotherapy without a long time delay could be an additional reason for favouring ovarian tissue cryopreservation. This concept is discussed in regard to two cases.
authors.affiliation: Department of Obstetrics and Gynecology, University of Cologne, Kerpener Strasse 34, 50931 Cologne, Germany.
authors.forename: Eugenia
authors.initials: E
authors.lastname: Isachenko
authors.forename: Gohar
authors.initials: G
authors.lastname: Rahimi
authors.forename: Vladimir
authors.initials: V
authors.lastname: Isachenko
authors.forename: Frank
authors.initials: F
authors.lastname: Nawroth
mesh_terms: D000328:Adult; D018929:Cell Culture Techniques; D015925:Cryopreservation; D005260:Female; D006801:Humans; D008540:Meiosis / Q000502:physiology*; D008677:Metaphase / Q000502:physiology*; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D010053:Ovary / Q000166:cytology / Q000502:physiology