title: Identification of an oxidative stress-sensitive protein from Campylobacter jejuni, homologous to rubredoxin oxidoreductase/rubrerythrin.
abstract: An oxidative stress-sensitive protein was found in the microaerophile Campylobacter jejuni. A novel 27-kDa protein was found to decrease concomitantly with a decrease in viability from either exogenous H(2)O(2) stress or endogenous oxidative stresses in aerobic conditions. Sequence analyses revealed that the 27-kDa protein was identical to Cj0012c in C. jejuni NCTC11168 and its deduced 215 amino acid sequence has similarity to two non-heme iron proteins found in other bacteria, rubredoxin oxidoreductase (Rbo) and rubrerythrin (Rbr). Thus, we designated the protein as Rrc (Rbo/Rbr-like protein of C. jejuni). In H(2)O(2)-treated cells, Western blot analysis showed some bands smaller than Rrc, and RT-PCR showed similar expression of Rrc mRNA to the control without treatment, suggesting that the sensitive response of Rrc to oxidative stress is due to degradation of the protein.
authors.affiliation: Division of Biomedical Food Research, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Shizunobu
authors.initials: S
authors.lastname: Igimi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Katayama
authors.forename: Shigeki
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Fumio
authors.initials: F
authors.lastname: Amano
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D016123:Campylobacter jejuni / Q000187:drug effects / Q000378:metabolism*; D005288:Ferredoxins / Q000737:chemistry; D006422:Hemerythrin; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000378:metabolism; D012416:Rubredoxins; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D005288:Ferredoxins; D006422:Hemerythrin; D012333:RNA, Messenger; D012416:Rubredoxins; C055857:rubrerythrins; D006861:Hydrogen Peroxide; C023091:rubredoxin-NAD+ reductase; D009247:NADH, NADPH Oxidoreductases