[The expression of acetyl-heparanase, laminin and laminin receptor and their role in the metastasis of ovarian cancer].
OBJECTIVE
To explore the expressions of acetyl-heparanase mRNA, laminin ( LN) and laminin receptor ( LR) in 50 ovarian carcinoma, 33 ovarian carcinoma with lymph node metastasis, and 10 serous ovarian cystadenoma as well as their role in the metastasis of ovarian cancer.


METHODS
The transcription level of acetyl-heparanase mRNA, expressions of LN and LR were detected by in situ hibridization and immunohistochemical staining, respectively.


RESULTS
The transcription level of acetyl-heparanase mRNA in ovarian carcinoma tissue and metastatic lymph nodes increased significantly, but its expression in primary focus was notably higher than that in metastatic lymph nodes (P < 0. 05 ). There was low expression of acetyl-heparanase mRNA in serous ovarian cystadenoma. The expression of acetyl-heparanase mRNA in malignant and benign tumor tissues had markedly difference (P < 0. 01 ). Expressions LN in both tissues mentioned above decreased while LR expression was high. The expression of acetyl-heparanase mRNA was negative correlation with that of LN, while positive with that of LR.


CONCLUSIONS
The correlation among expressions of acetyl-heparanase mRNA, LN and LR suggests that heparanase is involved in the growth, invasion and metastasis of ovarian carcinoma.
Liu
Yu
Y
Department of Obstetrics and Gynecology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Xin
Xiao-yan
XY
Han
Liang-fu
LF
Wang
De-tang
DT
D005260:Female; D005966:Glucuronidase / Q000235:genetics* / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007797:Laminin / Q000032:analysis*; D009362:Neoplasm Metastasis; D010051:Ovarian Neoplasms / Q000737:chemistry / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis; D017474:Receptors, Laminin / Q000032:analysis* / Q000502:physiology; D000099175:Heparanase
D005966:Glucuronidase; D007797:Laminin; D012333:RNA, Messenger; D017474:Receptors, Laminin; D000099175:Heparanase