[A novel cis-acting enhancer element between CKLF and CKLFSF1 genes].
OBJECTIVE
To explore the roles of the CKLF gene and CKLFSF1 gene sequence (CCS) in transcriptional regulation.


METHODS
The target gene fragment was amplified by PCR and then inserted into pGL3-basic and pGL3-SV40 containing luciferase reporter vector gene to construct pGL3-basic-CCS and pGL3-SV40-CCS. Using liposome-mediated method, four recombinant plasmids were respectively transfected into Hela cells. Transient expression was analyzed.


RESULTS
The luciferase assay indicated that the no luciferase activity was detected in Hela cells transtected with pGL3-basic and pGL3-basic-CCS. However, the luciferase activity was doubled when pGL3-SV40-CCS was transfected into Hela cells.


CONCLUSIONS
The CCS has no promoter activity, whereas some important cis-acting enhancer elements which modulate its downstream gene expression may exist within this sequence.
Xu
Ming-Xu
MX
Laboratory of Molecular Immunology, Human Disease Genomics, Peking University, Beijing 100083, China.
Lai
Mao-Yi
MY
Xia
Dong-Lan
DL
Wang
Ying
Y
Zhang
Ying-Mei
YM
Wang
Lu
L
Han
Wen-Ling
WL
D001483:Base Sequence; D004742:Enhancer Elements, Genetic; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics; D011401:Promoter Regions, Genetic; D014162:Transfection
D008156:Luciferases