Resistance to scrapie in PrP ARR/ARQ heterozygous sheep is not caused by preferential allelic use.
BACKGROUND
In sheep, susceptibility to scrapie, which is similar to human prion diseases such as Kuru and variant Creutzfeldt-Jakob disease (vCJD), is determined by prion protein (PrP) gene (Prnp) polymorphisms. Sheep with genotype ARQ/ARQ, denoting polymorphisms at codons 136, 154, and 171, are susceptible, whereas those with genotypes ARR/ARQ and ARR/ARR are resistant, indicating dominance of ARR over the ARQ allele.


OBJECTIVE
Based on familial CJD E200K, 129V, where preferential use of the 200E allele in EK heterozygous individuals confers resistance, heterozygous ARR/ARQ sheep were used to test the hypothesis that resistance is caused by preferential use of the ARR allele.


METHODS
After assessment of equivalent PrP expression across genotypes, allele use was analysed by sequencing reverse transcription polymerase chain reaction derived DNA clones containing the Prnp gene coding sequence.


RESULTS
The ARR to ARQ ratio was 1.1 in 133 clones, representing Prnp mRNA from three ARR/ARQ sheep, indicating equal use of both alleles.


CONCLUSIONS
Dominance of the resistant associated allele in sheep scrapie involves mechanisms other than the absence of PrP derived from the disease associated ARQ allele.
Caplazi
P A
PA
Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USA.
O'Rourke
K I
KI
Baszler
T V
TV
D000483:Alleles; D000818:Animals; D005455:Fluorescent Antibody Technique; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006579:Heterozygote; D011328:Prions / Q000235:genetics* / Q000378:metabolism; D012608:Scrapie / Q000235:genetics* / Q000378:metabolism; D012756:Sheep
D011328:Prions