Pressure-inactivated FMDV: a potential vaccine.
Foot-and-mouth disease virus (FMDV) is the causative agent of the foot-and-mouth disease (FMD). Alternative FMD vaccines have been pursued due to important disadvantages of the one currently in use. High hydrostatic pressure (HP) has been observed to inactivate some viruses. Here, we investigated the effects of HP on FMDV O1 Campos-Vallée (CVa) infectivity. A treatment consisting of 2.5 kbar at -15 degrees C and 1M urea, completely abolished FMDV infectivity, maintaining the integrity of its capsid structure. Moreover, its ability to elicit neutralizing antibody production in rabbits was preserved. Taken together, our results suggest that HP could be a safe, simple, cheap and reproducible way for viral vaccine production.
Ishimaru
Daniella
D
Departamento de Bioquímica Médica, Centro Nacional de Ressonância Magnética Nuclear de Macromoléculas, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-590, Brazil.
Sá-Carvalho
Daniel
D
Silva
Jerson L
JL
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D005536:Foot-and-Mouth Disease / Q000517:prevention & control*; D029544:Foot-and-Mouth Disease Virus / Q000254:growth & development / Q000276:immunology* / Q000648:ultrastructure; D006874:Hydrostatic Pressure; D009500:Neutralization Tests; D011817:Rabbits; D013696:Temperature; D014508:Urea; D014611:Vaccination / Q000379:methods*; D014765:Viral Vaccines / Q000276:immunology*; D038301:Virus Inactivation; D014779:Virus Replication
D000914:Antibodies, Viral; D014765:Viral Vaccines; D014508:Urea