Non-invasive online detection of nitric oxide from plants and some other organisms by mass spectrometry.
As nitric oxide (NO) is a key messenger in many organisms, reliable techniques for the detection of NO are essential. Here, it is shown that a combination of membrane inlet mass spectrometry (MIMS) and restriction capillary inlet mass spectrometry (RIMS) allows for the fast, specific, and non-invasive online detection of NO that has been emitted from tissue cultures of diverse organisms, or from whole plants. As an advantage over other NO assays, MIMS/RIMS discriminates nitrogen isotopes and simultaneously measures NO and O(2) (and other gases) from the same sample. MIMS/RIMS technology may thus help to identify the source of gaseous NO in cells, and elucidate the relationship between primary gas metabolism and NO formation. Using RIMS, it is demonstrated that the novel fungicide F 500((R)) triggers NO production in plants.
Conrath
Uwe
U
Plant Molecular Biology Department, Institute for Cellular and Molecular Botany, The University of Bonn, 1 Kirschallee, 53115 Bonn, Germany. uwe.conrath@bio3.rwth-aachen.de
Amoroso
Gabriele
G
Köhle
Harald
H
Sültemeyer
Dieter F
DF
D005658:Fungi / Q000737:chemistry; D005659:Fungicides, Industrial / Q000494:pharmacology; D013058:Mass Spectrometry / Q000379:methods*; D009569:Nitric Oxide / Q000032:analysis*; D009587:Nitrogen Isotopes; D010100:Oxygen / Q000032:analysis; D010944:Plants / Q000737:chemistry*
D005659:Fungicides, Industrial; D009587:Nitrogen Isotopes; D009569:Nitric Oxide; D010100:Oxygen