title: Caspase-3 and caspase-9 mediate developmental apoptosis in the mouse olfactory system.
abstract: Neuronal apoptosis is a key component in the sculpting of tissues during embryonic and postnatal development and is driven largely by the action of caspases. In the mouse olfactory system, caspase-3 and -9 are expressed in olfactory receptor neurons (ORNs) of adult mice, and their selective retrograde activation drives ORN apoptosis following ablation of their target, the olfactory bulb (OB). Here, we show that both of these caspases are expressed at the earliest stages of ORN embryonic development, and their expression is concentrated in outgrowing ORN axons. The retention, in null mice for both caspases, of a population of ORNs that would normally undergo developmental apoptosis beginning at E13 of development, results in a permanently expanded population of ORNs. In turn, in some caspase-3 null mice, the ORN target organ, the OB, also develops abnormally, resulting in the formation of secondary, apparently functional, extracranial ectopic OBs.
authors.affiliation: Department of Zoology, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada.
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Cowan
authors.forename: A Jane
authors.initials: AJ
authors.lastname: Roskams
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3; D053453:Caspase 9; D020169:Caspases / Q000235:genetics / Q000502:physiology*; D002452:Cell Count / Q000379:methods; D048429:Cell Size / Q000235:genetics; D004622:Embryo, Mammalian; D005838:Genotype; D007120:Immunochemistry / Q000379:methods; D020287:In Situ Nick-End Labeling / Q000379:methods; D051379:Mice; D018345:Mice, Knockout; D009833:Olfactory Pathways / Q000166:cytology* / Q000502:physiology; D018034:Olfactory Receptor Neurons / Q000502:physiology*
chemical_list: C505409:Casp3 protein, mouse; C506434:Casp9 protein, mouse; D053148:Caspase 3; D053453:Caspase 9; D020169:Caspases