Isolation of gibberellin metabolic pathway genes from barley and comparative mapping in barley, wheat and rice.
Gene sequences encoding gibberellin (GA) biosynthetic and catabolic enzymes were isolated from "Himalaya" barley. These genes account for most of the enzymes required for the core pathway of GA biosynthesis as well as for the first major catabolic enzyme. By means of DNA gel blot analysis, we mapped coding sequences to chromosome arms in barley and wheat using barley-wheat chromosome addition lines, nulli-tetrasomic substitution and ditelosomic lines of wheat. These same sequences were used to identify closely related sequences from rice, which were mapped in silico, thereby allowing their syntenic relationship with map locations in barley and wheat to be investigated. Determination of the chromosome arm locations for GA metabolic genes provides a framework for future studies investigating possible identity between GA metabolic genes and dwarfing genes in barley and wheat.
Spielmeyer
Wolfgang
W
CSIRO Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia. Wolfgang.Spielmeyer@csiro.au
Ellis
Marc
M
Robertson
Masumi
M
Ali
Shahjahan
S
Lenton
John R
JR
Chandler
Peter M
PM
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D016000:Cluster Analysis; D004798:Enzymes / Q000235:genetics* / Q000378:metabolism; D015723:Gene Library; D005875:Gibberellins / Q000378:metabolism*; D001467:Hordeum / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D026801:Synteny / Q000235:genetics; D014908:Triticum / Q000235:genetics*
D004798:Enzymes; D005875:Gibberellins