An in vivo method for recording single unit activity in lumbar spinal cord in mice anesthetized with a volatile anesthetic.
We describe a method to record single unit neuronal activity from mouse spinal cord using volatile anesthesia. The small size of the mouse can complicate usual methods that are used for single-unit recording in rats, but simple modifications can significantly increase the number of successful recordings. Stabilization of the vertebral column is particularly important, as are adequate ventilation of the animal, control of body temperature and accurate determination of anesthetic concentrations in respiratory gas samples.
Cuellar
Jason M
JM
Section of Neurobiology, Physiology and Behavior, University of California, Davis, Davis, CA 95616, USA.
Antognini
Joseph F
JF
Carstens
Earl
E
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology*; D000758:Anesthesia / Q000379:methods*; D000776:Anesthesiology / Q000295:instrumentation; D018685:Anesthetics, Inhalation / Q000378:metabolism / Q000493:pharmacokinetics; D000818:Animals; D001831:Body Temperature / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods*; D008159:Lumbar Vertebrae; D008297:Male; D008465:Mechanoreceptors / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009482:Neurophysiology / Q000295:instrumentation / Q000379:methods*; D019635:Neurosurgical Procedures / Q000295:instrumentation / Q000379:methods; D009619:Nociceptors / Q000187:drug effects / Q000502:physiology; D010812:Physical Stimulation; D012121:Respiration, Artificial / Q000295:instrumentation / Q000379:methods; D013116:Spinal Cord / Q000187:drug effects / Q000502:physiology*; D013131:Spine / Q000033:anatomy & histology / Q000502:physiology
D018685:Anesthetics, Inhalation