Increased expression and altered location of annexin IV in renal clear cell carcinoma: a possible role in tumour dissemination.
The proteome of renal cell carcinoma and non-neoplastic kidney tissue was analysed from 12 patients by two-dimensional polyacrylamide gel electrophoresis to search for differentially expressed proteins in the tumour. Annexin IV was identified to be up-regulated in tumour cells. These patients and further 11 were characterized by RT-PCR. We found an increased amount of annexin IV mRNA. Immunohistochemical analysis revealed an altered localization of annexin IV in tumour cells. Additionally we demonstrate that over-expressed annexin IV promotes cell migration in a carcinoma model system. From these results above it seems possible that annexin IV plays an important role in the morphological diversification and dissemination of the clear cell renal cell carcinoma.
Zimmermann
Uwe
U
Department of Urology, University of Greifswald, D-17487 Greifswald, Germany.
Balabanov
Stefan
S
Giebel
Jürgen
J
Teller
Steffen
S
Junker
Heike
H
Schmoll
Dieter
D
Protzel
Chris
C
Scharf
Christian
C
Kleist
Britta
B
Walther
Reinhard
R
D018262:Adenocarcinoma, Clear Cell / Q000378:metabolism*; D017314:Annexin A4 / Q000096:biosynthesis*; D002292:Carcinoma, Renal Cell / Q000378:metabolism*; D002465:Cell Movement; D015180:Electrophoresis, Gel, Two-Dimensional; D049452:Green Fluorescent Proteins; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000378:metabolism*; D008164:Luminescent Proteins / Q000378:metabolism; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
D017314:Annexin A4; D008164:Luminescent Proteins; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins