title: The proximal 2-kb of the Hoxa3 promoter directs gene expression in distinct branchial compartments and cranial ganglia.
abstract: Developing structures such as hindbrain, neural crest cells or spinal cord express Hoxa3. Here, we have investigated the regulatory role of a 2-kb fragment spanning the proximal promoter of Hoxa3 by a reporter-based approach in mice. We show that this fragment promotes reporter activity in ganglionic and branchial compartments known to express Hoxa3 but for which no cis-regulatory elements have been identified so far. We also show that the 2-kb promoter fragment is active in rhombomere 4 and in the ganglion of the cranial nerve complex VII/VIII that are devoid of Hoxa3 expression.
authors.affiliation: Unit of Developmental Genetics, Université Catholique de Louvain, 73 (box 82) Avenue Mounier, Brussels 1200, Belgium.
authors.forename: Nata Y S-G
authors.initials: NY
authors.lastname: Diman
authors.forename: Ethel
authors.initials: E
authors.lastname: Chauvier
authors.forename: Nathalie
authors.initials: N
authors.lastname: Pacico
authors.forename: Jacques J
authors.initials: JJ
authors.lastname: Picard
authors.forename: René
authors.initials: R
authors.lastname: Rezsohazy
mesh_terms: D000818:Animals; D001934:Branchial Region / Q000196:embryology / Q000378:metabolism*; D003391:Cranial Nerves / Q000196:embryology / Q000378:metabolism*; D004622:Embryo, Mammalian; D004627:Embryonic Induction; D005725:Ganglia, Autonomic / Q000196:embryology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012249:Rhombencephalon / Q000196:embryology / Q000378:metabolism*
chemical_list: D018398:Homeodomain Proteins; C080331:Hoxa3 protein, mouse