Liquid chromatographic determination including simultaneous "on-cartridge" separation of ranitidine cisapride drug combinations from paediatric plasma samples using an automated solid-phase extraction procedure.
HPLC methodology was investigated for the simultaneous determination of cisapride and ranitidine in small volume paediatric plasma samples. Such a simultaneous determination proved difficult due to the small sample volumes, the low concentrations of the drugs and the different log P values of the two compounds. The two drugs and their respective internal standards were separated "on-cartridge" using HLB Solid Phase Extraction cartridges and the samples quantified by individual HPLC methodologies. The technique has been applied successfully to 60 paediatric plasma samples containing both cisapride and ranitidine.
Hare
L G
LG
Children's Medicines Research Group, The School of Pharmacy, The Queen's University of Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT97BL, Northern Ireland, UK.
Mitchel
D S
DS
Millership
J S
JS
Collier
P S
PS
McElnay
J C
JC
Shields
M D
MD
Carson
D J
DJ
Fair
R
R
D001331:Automation; D002648:Child; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020117:Cisapride / Q000097:blood*; D005765:Gastrointestinal Agents / Q000097:blood*; D006801:Humans; D011899:Ranitidine / Q000097:blood*; D015203:Reproducibility of Results
D005765:Gastrointestinal Agents; D011899:Ranitidine; D020117:Cisapride