Isoflavones found in Korean soybean paste as 3-hydroxy-3-methylglutaryl Coenzyme A reductase inhibitors.
3-Hydroxy-3-methylglutaryl CoA (HMG-CoA) reductase is the rate-limiting enzyme in the biosynthesis of cholesterol in mammals. Some microbial metabolites have been found to be HMG-CoA reductase inhibitors. Korean soybean paste is a unique food fermented by many microorganisms. The enzymatic method using the catalytic domain of Syrian hamster HMG-CoA reductase was employed for the screening of HMG-CoA reductase inhibitors. Soybean paste extract was fractionated by vacuum liquid chromatography. Fractions showing relatively high HMG-CoA reductase inhibition were further purified through Sephadex LH-20 column chromatography and C18 preparative HPLC, and the inhibitory compounds were identified as genistein, daidzein, and glycitein.
Sung
Jang Hoon
JH
Department of Food Science and Technology, School of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, Seoul, Korea.
Choi
Seung Jun
SJ
Lee
Soon Won
SW
Park
Kwan Hwa
KH
Moon
Tae Wha
TW
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D019833:Genistein / Q000032:analysis; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D025782:Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent / Q000378:metabolism; D007529:Isoflavones / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013058:Mass Spectrometry; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry*
D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D007529:Isoflavones; D010936:Plant Extracts; C004742:daidzein; C086566:glycitein; D019833:Genistein; D025782:Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent