Mutational analysis of the Drosophila DNA repair and recombination gene mei-9.
Drosophila mei-9 is essential for several DNA repair and recombination pathways, including nucleotide excision repair (NER), interstrand crosslink repair, and meiotic recombination. To better understand the role of MEI-9 in these processes, we characterized 10 unique mutant alleles of mei-9. These include a P-element insertion that disrupts repair functions but not the meiotic function; three nonsense mutations, one of which has nearly wild-type levels of protein; three missense mutations, one of which disrupts the meiotic function but not repair functions; two small in-frame deletions; and one frameshift.
Yildiz
Ozlem
O
Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
Kearney
Hutton
H
Kramer
Benjamin C
BC
Sekelsky
Jeff J
JJ
D000483:Alleles; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D018389:Codon, Nonsense; D004249:DNA Damage; D004252:DNA Mutational Analysis; D004260:DNA Repair; D029721:Drosophila Proteins / Q000235:genetics* / Q000502:physiology*; D004331:Drosophila melanogaster / Q000235:genetics*; D016368:Frameshift Mutation; D017353:Gene Deletion; D008540:Meiosis; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D020125:Mutation, Missense; D009687:Nuclear Proteins / Q000235:genetics* / Q000502:physiology*; D011995:Recombination, Genetic; D017386:Sequence Homology, Amino Acid; D020798:Two-Hybrid System Techniques; D014466:Ultraviolet Rays
D018389:Codon, Nonsense; D029721:Drosophila Proteins; C097527:MEI-9 protein, Drosophila; D009687:Nuclear Proteins