[In-vivo dynamic changes of antigen presenting cells infected with recombinant attenuated Salmonella typhimurium expressing green fluorescent protein].
OBJECTIVE
To clarify in-vivo early dynamic changes of antigen-presenting cells (APCs) infected with recombinant attenuated Salmonella typhimurium.


METHODS
BALB/c mice were orally infected with recombinant attenuated Salmonella typhimurium expressing green fluorescent protein (GFP) X4550 (pYAGFP). Peritoneal macrophages were taken out 3 days after infection, cultured for 24 hours, and observed under the fluorescent microscope. Moreover, BALB/c mice were also infected intravenously with X4550 (pYAGFP) and low density cells (LDCs) were isolated and prepared from mouse spleen and liver 3,6 and 12 hours after infection. Infection rates of macrophages and dendritic cells (DCs) were analyzed by flow cytometry.


RESULTS
About 50% peritoneal macrophages were X4550 (pYAGFP) positive. Infection rates of macrophages in spleen and liver were about 20%-40%. As for the DCs, the infection rates in spleen and liver were about 4%-10% and 10%-20%, respectively.


CONCLUSIONS
Recombinant attenuated Salmonella can be captured by APCs in-vivo in early infection, which provides a precondition for inducing effective immune responses.
Wang
Fang
F
Key Laboratory of Animal Infectious Disease of Ministry of Agriculture, Yangzhou University, Yangzhou 225009, China. rwangfang@hotmail.com
Jiao
Xin-an
XA
Gu
Jian
J
Ma
Li
L
Lo-Man
Richard
R
Leclerc
Claude
C
Liu
Xiu-fan
XF
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000382:microbiology*; D005260:Female; D049452:Green Fluorescent Proteins; D008099:Liver / Q000382:microbiology; D008164:Luminescent Proteins / Q000235:genetics; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D012486:Salmonella typhimurium / Q000472:pathogenicity*; D013154:Spleen / Q000382:microbiology
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins