Direct measure of functional importance visualized atom-by-atom for photoactive yellow protein: application to photoisomerization reaction.
Photoreceptor proteins serve as efficient nano-machines for the photoenergy conversion and the photosignal transduction of living organisms. For instance, the photoactive yellow protein derived from a halophilic bacterium has the p-coumaric acid chromophore, which undergoes an ultrafast photoisomerization reaction after light illumination. To understand the structure-function relationship at the atomic level, we used a computational method to find functionally important atoms for the photoisomerization reaction of the photoactive yellow protein. In the present study, a "direct" measure of the functional significance was quantitatively evaluated for each atom by calculating the partial atomic driving force for the photoisomerization reaction. As a result, we revealed the reaction mechanism in which the specific role of each functionally important atom has been well characterized in a systematic manner. In addition, we observed that this mechanism is strongly conserved during the thermal fluctuation of the photoactive yellow protein. We compared the experimental data of fluorescence decay constant of several different mutants and the present analysis. As a result, we found that the reaction rate constant is decreased when a large positive driving force is missing.
Yamada
Atsushi
A
Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya, Japan.
Ishikura
Takakazu
T
Yamato
Takahisa
T
D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D019295:Computational Biology; D003373:Coumaric Acids / Q000737:chemistry; D007536:Isomerism; D008958:Models, Molecular; D015394:Molecular Structure; D010777:Photochemistry; D020283:Photoreceptors, Microbial / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D001426:Bacterial Proteins; D003373:Coumaric Acids; D020283:Photoreceptors, Microbial; C050162:photoactive yellow protein, Bacteria