Cloning and expression of type XII collagen isoforms during bovine adipogenesis.
In order to isolate candidate genes involved in bovine adipocyte differentiation, we have constructed a subtraction library from a clonal bovine intra-muscular pre-adipocyte (BIP) cell line using the suppression subtractive hybridization method. We have isolated a set of subtracted cDNA fragments whose respective mRNA levels are up-regulated during the adipogenic differentiation of BIP cells, and cloned cDNAs from a differentiated BIP-lambda ZAP II cDNA library. Two cDNA clones were highly homologous to the sequence of mouse and human type XII collagen alpha-1, determined by a BLAST homology search. As type XII collagen has been reported to have four types of splicing isoform, two clones were determined to be XII-1 and XII-2 splicing isoforms, respectively, because of a difference in the C-terminal NC1 domain. From the expression analysis of type XII collagen, the XIIA-2 isoform was mainly expressed in differentiated BIP cells and adipose tissues. Although the function of type XII collagen has not been established as yet, these results suggest that type XII collagen may be associated with adipocyte differentiation and adipose formation in cattle and is a potentially useful marker for adipogenesis.
Tahara
Koji
K
Cellular Biology Laboratory, Faculty of Agriculture, Tohoku University, Sendai, 981-8555, Japan. tahara@bml.co.jp
Aso
Hisashi
H
Yamasaki
Tadashi
T
Rose
Michael T
MT
Takasuga
Akiko
A
Sugimoto
Yoshikazu
Y
Yamaguchi
Takahiro
T
Tahara
Koji
K
Takano
Shoichi
S
D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000273:Adipose Tissue / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D003001:Cloning, Molecular; D024082:Collagen Type XII / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D014018:Tissue Distribution
D024082:Collagen Type XII; D020033:Protein Isoforms