On line biomonitors used as a tool for toxicity reduction evaluation of in situ groundwater remediation techniques.
Success of groundwater remediation is typically controlled via snapshot analysis of selected chemical substances or physical parameters. Biological parameters, i.e. ecotoxicological assays, are rarely employed. Hence the aim of the study was to develop a bioassay tool, which allows an on line monitoring of contaminated groundwater, as well as a toxicity reduction evaluation (TRE) of different remediation techniques in parallel and may furthermore be used as an additional tool for process control to supervise remediation techniques in a real time mode. Parallel testing of groundwater remediation techniques was accomplished for short and long time periods, by using the energy dependent luminescence of the bacterium Vibrio fischeri as biological monitoring parameter. One data point every hour for each remediation technique was generated by an automated biomonitor. The bacteria proved to be highly sensitive to the contaminated groundwater and the biomonitor showed a long standing time despite the highly corrosive groundwater present in Bitterfeld, Germany. The bacterial biomonitor is demonstrated to be a valuable tool for remediation success evaluation. Dose response relationships were generated for the six quantitatively dominant groundwater contaminants (2-chlortoluene, 1,2- and 1,4-dichlorobenzene, monochlorobenzene, ethylenbenzene and benzene). The concentrations of individual volatile organic chemicals (VOCs) could not explain the observed effects in the bacteria. An expected mixture toxicity was calculated for the six components using the concept of concentration addition. The calculated EC(50) for the mixture was still one order of magnitude lower than the observed EC(50) of the actual groundwater. The results pointed out that chemical analysis of the six most quantitative substances alone was not able to explain the effects observed with the bacteria. Thus chemical analysis alone may not be an adequate tool for remediation success evaluation in terms of toxicity reduction.
Küster
Eberhard
E
Department of Chemical Ecotoxicology, UFZ Centre for Environmental Research Leipzig-Halle in the Helmholtz-Association, Permoserstr. 15, 04318 Leipzig, Germany. eberhard.kuester@ufz.de
Dorusch
Falk
F
Vogt
Carsten
C
Weiss
Holger
H
Altenburger
Rolf
R
D048908:Aliivibrio fischeri / Q000187:drug effects* / Q000302:isolation & purification*; D001673:Biodegradation, Environmental; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D015169:Colony Count, Microbial / Q000295:instrumentation / Q000379:methods; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods; D009862:Online Systems; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000494:pharmacology*; D018508:Water Purification / Q000295:instrumentation* / Q000379:methods
D014874:Water Pollutants, Chemical