[Intracellular localization of thiamine-binding proteins in the liver and kidneys of rats].
The distribution of thiamine-binding and thiamine triphosphatase activity typical of thiamine-binding proteins was studied in intracellular structures of rats liver and kidneys. It was found that the fraction of microsomes has the highest rate of specific thiamine-binding activity amide fractions of subcellular structures that was isolated using differential centrifugation in the both organs. Hydrolysis of thiamine triphosphate (pH 7.4) was also extremely active in these structures. The results of our research allow to make a conclusion that subcellular structures precipitated as fraction of microsomes (endoplasmic reticulum and vesicled parts of plasma membranes) are the sites of the most probable localisation of thiamine-binding proteins of liver and kidneys.
Ianchiĭ
O R
OR
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv. oyanchiy@biochem.kiev.ua
Parkhomenko
Iu M
IuM
Donchenko
G V
GV
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000201:enzymology / Q000378:metabolism; D042541:Intracellular Space / Q000201:enzymology / Q000378:metabolism*; D007668:Kidney / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013837:Thiamin-Triphosphatase / Q000378:metabolism
D002352:Carrier Proteins; C026989:thiamine-binding protein; D013837:Thiamin-Triphosphatase