title: Human amoebic hepatic abscess: in situ interactions between trophozoites, macrophages, neutrophils and T cells.
abstract: Amoebic liver abscesses (ALA) are the most frequent and severe extraintestinal clinical presentations of amoebiasis. During the early establishment of amoebae in the liver parenchyma, as well as during the extension of the tissue necrosis, parasites interact with the parenchymal liver cells and, as a consequence of these interactions, hepatocytes can be destroyed and host immune cells can become activated. However, little is known about the nature of these interactions in the liver or about the factors involved in the local immune response. In this investigation we studied the localization of Entamoeba histolytica trophozoites, TCD4+, TCD8+ cells, CD68+ macrophages and CD15+ neutrophils in human ALA using immunohistochemical techniques. Trophozoites were found close to undamaged hepatocytes in both lysed and non-lysed areas with either sparse or abundant inflammatory infiltrate. CD8+ cells were more abundant than CD4+ T cells. CD 68+ macrophages and CD15+ neutrophils were also detected, suggesting that neutrophils, macrophages and T cells might be related to the local host immune mechanisms in ALA. We also found that E. histolytica possesses proteins recognized by antibodies raised against inducible nitric oxide synthase.
authors.affiliation: Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, México. jventur@correo.uaa.mx
authors.forename: J
authors.initials: J
authors.lastname: Ventura-Juárez
authors.forename: R A
authors.initials: RA
authors.lastname: Jarillo-Luna
authors.forename: E
authors.initials: E
authors.lastname: Fuentes-Aguilar
authors.forename: A
authors.initials: A
authors.lastname: Pineda-Vázquez
authors.forename: L
authors.initials: L
authors.lastname: Muñoz-Fernández
authors.forename: J I
authors.initials: JI
authors.lastname: Madrid-Reyes
authors.forename: R
authors.initials: R
authors.lastname: Campos-Rodríguez
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000469:parasitology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000469:parasitology*; D004748:Entamoeba histolytica / Q000201:enzymology / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D016256:Lewis X Antigen / Q000276:immunology; D008101:Liver Abscess, Amebic / Q000276:immunology* / Q000469:parasitology; D008264:Macrophages / Q000276:immunology / Q000469:parasitology; D009504:Neutrophils / Q000276:immunology / Q000469:parasitology; D019001:Nitric Oxide Synthase / Q000276:immunology; D052247:Nitric Oxide Synthase Type II; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D016256:Lewis X Antigen; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C496319:NOS2 protein, human