title: [Preparation and characterization of monoclonal antibodies against human transmembrane activator and calcium modulator and cyclophilin ligand interactor].
abstract: OBJECTIVE
  To prepare monoclonal antibodies against human TACI and study the distribution of TACI on peripheral blood nuclear cells(PBMCs).
  
  
  METHODS
  Murine mAbs were prepared by hybridoma technique. The distribution of TACI on PBMCs were identified by immunofluorescence staining, FCM analysis as well as immunohistochemical staining.
  
  
  RESULTS
  Two hybridoma cell lines secreting mAbs against TACI were obtained. The titers of ascitic mAbs was 10(-7) and their Ig subclasses were IgG1. Both could recognize natural TACI on T cells. In the PHA activated models, TACI was expressed mainly on activated CD4(+) T cells.
  
  
  CONCLUSIONS
  Two mAbs can recognize natural TACI, which will be of greater value in research on the structure and functions of TACI.
authors.affiliation: Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Xue-Song
authors.initials: XS
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhu
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Chen
authors.forename: Yun-Xin
authors.initials: YX
authors.lastname: Cao
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Xu
authors.forename: Yi
authors.initials: Y
authors.lastname: Hu
authors.forename: Ying
authors.initials: Y
authors.lastname: Tian
authors.forename: Bo-Quan
authors.initials: BQ
authors.lastname: Jin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology; D002118:Calcium / Q000378:metabolism; D021983:Cyclophilins; D006801:Humans; D006825:Hybridomas; D008807:Mice, Inbred BALB C; D053303:Transmembrane Activator and CAML Interactor Protein
chemical_list: D000911:Antibodies, Monoclonal; D053303:Transmembrane Activator and CAML Interactor Protein; D021983:Cyclophilins; D002118:Calcium