Crystallization and preliminary X-ray diffraction analysis of an acidic phospholipase A(2) complexed with p-bromophenacyl bromide and alpha-tocopherol inhibitors at 1.9- and 1.45-A resolution.
An acidic phospholipase A(2) (PLA(2)) isolated from Bothrops jararacussu snake venom was crystallized with two inhibitors: alpha-tocopherol (vitamin E) and p-bromophenacyl bromide (BPB). The crystals diffracted at 1.45- and 1.85-A resolution, respectively, for the complexes with alpha-tocopherol and p-bromophenacyl bromide. The crystals are not isomorphous with those of the native protein, suggesting the inhibitors binding was successful and changes in the quaternary structure may have occurred.
Takeda
Agnes A S
AA
Departamento de Física e Biofísica, Instituto de Biociências, UNESP, Dist. Rubiao Jr. s/n-C.P. 510, CEP 18618-000, Botucatu-São Paulo, Brazil.
dos Santos
Juliana I
JI
Marcussi
Silvana
S
Silveira
Lucas B
LB
Soares
Andreimar M
AM
Fontes
Marcos R M
MR
D000098:Acetophenones / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001665:Binding Sites; D017837:Bothrops; D003435:Crotalid Venoms / Q000737:chemistry / Q000201:enzymology*; D003460:Crystallization; D004791:Enzyme Inhibitors / Q000378:metabolism; D010741:Phospholipases A / Q000737:chemistry* / Q000378:metabolism*; D014810:Vitamin E / Q000378:metabolism*; D014961:X-Ray Diffraction
D000098:Acetophenones; D000975:Antioxidants; D003435:Crotalid Venoms; D004791:Enzyme Inhibitors; D014810:Vitamin E; D010741:Phospholipases A; C006821:4-bromophenacyl bromide