title: Overexpression of the neuropilin 1 (NRP1) gene correlated with poor prognosis in human glioma.
abstract: We studied whether the expression of the Neuropilin (NRP) gene was correlated with clinicopathological features in glioma. We examined the gene expression of vascular endothelial growth factor (VEGF)-A, Flt-1, KDR, NRP1 and NRP2 in 37 gliomas by real time reverse transcriptase PCR (real time RT-PCR) as well as immunohistochemical analysis. The vascular counts of each tumor were evaluated by anti-CD34 antibody. NRP1 mRNA overexpression was significantly higher in neoplastic tissue compared to normal brain tissue samples. The higher grade of glioma overexpressed the NRP1 gene significantly (p=0.0015). The glioma patients with NRP1 overexpression showed a poorer prognosis (p=0.0202) than those without such overexpression. NRP1 was observed in the glioma cells by immunohistochemical analyses. VEGF-A and VEGFR overexpression did not show any correlation with the clinicopathological features, including NRP expression. These results suggest that NRP1 overexpression, rather than VEGF-A or VEGFR, contributes to tumor progression and has clinical significance for glioma.
authors.affiliation: Department of Pathology, Tokai University, School of Medicine, Bohseidai, Isehara, Kanagawa 259-1143, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Osada
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Tokunaga
authors.forename: Masatake
authors.initials: M
authors.lastname: Nishi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hatanaka
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Abe
authors.forename: Atsushi
authors.initials: A
authors.lastname: Tsugu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kijima
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Yamazaki
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Ueyama
authors.forename: Masato
authors.initials: M
authors.lastname: Nakamura
mesh_terms: D000328:Adult; D001932:Brain Neoplasms / Q000098:blood supply / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015870:Gene Expression; D005910:Glioma / Q000098:blood supply / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism; D039942:Neuropilin-1 / Q000096:biosynthesis* / Q000235:genetics; D039943:Neuropilin-2 / Q000096:biosynthesis / Q000235:genetics; D011379:Prognosis; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000096:biosynthesis / Q000235:genetics; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000096:biosynthesis / Q000235:genetics
chemical_list: D039943:Neuropilin-2; D039942:Neuropilin-1; D040281:Vascular Endothelial Growth Factor Receptor-1; D040301:Vascular Endothelial Growth Factor Receptor-2