A study into the anti-microbial properties of an amino functionalised polymer using multi-parameter flow cytometry.
Fluorescent staining techniques were used to study the anti-microbial properties of aqueous suspensions of a novel, water insoluble amino functionalised polymer on three micro-organisms Pseudomonas fluorescens, Staphylococcus epidermidis and Saccharomyses cerevisiae. The mechanism of action was similar for each organism in that, after various contact times with the polymer, a progressive change in individual cell physiological state was measured using multi-parameter flow cytometry. The microbiocidal activity of this polymer may be similar to that of substances referred to as polycationic, amphipathic compounds (peptides, peptide derivatives and other polyamines).
Hewitt
Christopher J
CJ
School of Engineering (Chemical Engineering), Biochemical Engineering, The University of Birmingham, Edgbaston, B15 2TT, UK. c.j.hewitt@bham.ac.uk
Franke
Robert
R
Marx
Achim
A
Kossmann
Beate
B
Ottersbach
Peter
P
D000596:Amino Acids / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D005434:Flow Cytometry / Q000379:methods*; D008689:Methacrylates / Q000494:pharmacology*; D015999:Multivariate Analysis; D011108:Polymers / Q000494:pharmacology; D011551:Pseudomonas fluorescens / Q000166:cytology / Q000187:drug effects*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013212:Staphylococcus epidermidis / Q000166:cytology / Q000187:drug effects*
C495440:AMINA T 100 polymer; D000596:Amino Acids; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D008689:Methacrylates; D011108:Polymers