[Detection of C5a receptor expressions on human epiretinal membranes and cultured RPE cells by immunohistochemical staining].
OBJECTIVE
To observe the expression and distribution of C5a receptor(C5aR) on epiretinal membranes (ERM) tissue from patients with proliferative retreous body-retina (PVR) lesion and cultured retinal pigmentary epithelial (RPE) cells.


METHODS
The C5aR expressions on ERM tissue and cultured RPE cells were detected by ABC immunohistochemical staining with mouse anti-human C5aR mcAbs.


RESULTS
Expression of C5aR was found in ERM tissue and on cultured RPE cells as well as macrophages.


CONCLUSIONS
As a pro-inflammatory factor, binding of C5a to C5aR on RPE cells may play an important role in the development of PVR.
Wang
Yun-Peng
YP
Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Hui
Yan-Nian
YN
Wang
Yu-Sheng
YS
Wang
Hai-Yan
HY
D000818:Animals; D002478:Cells, Cultured; D015936:Complement C5a; D019773:Epiretinal Membrane; D015870:Gene Expression; D006801:Humans; D008264:Macrophages / Q000378:metabolism; D044087:Receptor, Anaphylatoxin C5a; D013194:Staining and Labeling
D044087:Receptor, Anaphylatoxin C5a; D015936:Complement C5a