[Study of antibody enzyme with acetylcholonesteraseactivity].
OBJECTIVE
To study anti-idiotype antibody(AId Ab)directed at mAb 3F3 Fab idiotypic determinant with acetylcholinesteras(AchE) activity.


METHODS
Anti-AchE mAb 3F3(IgG1) was digested by papain to prepare its Fab fragments. And then the Fab fragments were purified through Ach- and SPA-Sepharose 4B columns respectively. The BALB/c mice were immunized by using purified Fab fragments as immunogen to prepare AId Abs against the Fab fragmrnts. Catalytic activity of the AId Abs was detected by ELISA.


RESULTS
The purified mAb 3F3 Fab fragments were acquired by papain digestion and affinity chromatograpy successively. Enzyme catalysis ELISA detection showed that AId Abs to the Fab fragments had activity of AchE.


CONCLUSIONS
An AId Ab to mAb 3F3 Fab fragments has been prepared successfully, which opens up a novel way for treatment of pesticides poisoning.
Gao
Mei-Hua
MH
Department of Immunology, Medical College of Qingdao University, Qingdao 266021,China.
Fu
Feng-Hua
FH
Cheng
Ying
Y
Gong
Zhao-Long
ZL
Hu
Tao
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D000941:Antigens; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G