title: [Enhancement effect of IL-2 on the proliferation of cultured mammary carcinoma cell line Bcap-37].
abstract: OBJECTIVE
  To investigate the effects of IL-2 on the proliferation of human mammary carcinoma cell line Bcap-37 and explore its possible mechanism.
  
  
  METHODS
  Enhancement effect of IL-2 on proliferation of cultured Bcap-37 cells, the IL-2 expression on the cells, the expressions of IL-2Ralpha, beta, gamma mRNAs in the cells, the effect of IL-2 on DNA content at various periods of cell cycle and on Ca(2+) concentration in the cells were detected or observed by MTT colorimetry, immunohistochemical staining, RT-PCR, flow cytometry and laser scanning confocal microscope, respectively.
  
  
  RESULTS
  IL-2 of 1x10(5) to 1x10(6) U/L significantly stimulated the proliferation of cultured Bcap-37 cells. IL-2 was secreted and IL-2Ralpha, beta and gamma were expressed by cultured Bcap-37 cells. IL-2(1x10(5) U/L) increased ratio of Bcap-37 cells in G(2) and S phases and decreased the Ca(2+) release from Bcap-37 cells.
  
  
  CONCLUSIONS
  IL-2 significantly enhance the proliferation of mammary carcinoma cell line Bcap-37. The enhancement effect of IL-2 on Bcap-37 cell proliferation is possibly related to the expression of IL-2R and decreased Ca(2+) concentration in the cells.
authors.affiliation: Department of Physiology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Zi-Ying
authors.initials: ZY
authors.lastname: Zou
authors.forename: Yun-Long
authors.initials: YL
authors.lastname: Zhu
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Zhong
authors.forename: Gao-Feng
authors.initials: GF
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Zhou
mesh_terms: D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D053645:Interleukin-2 Receptor alpha Subunit; D061986:MCF-7 Cells
chemical_list: D007376:Interleukin-2; D053645:Interleukin-2 Receptor alpha Subunit