title: [Studies on the antithrombotic effects of anti-platelet mAb SZ-21 F(ab')(2) fragment].
abstract: OBJECTIVE
  To research the biological effect of the F(ab')(2) of anti-platelet GPIIIa mAb SZ-21.
  
  
  METHODS
  The F(ab')(2) fragment was obtained with papain digestion for 20 hours at 4 degrees Celsius, under acid condition(pH 5.5). The products were purified through DEAE-52 ion exchange column and the first eluted peak components were verified as F(ab')(2) by SDS-PAGE. The immunoactivity and its antithrombotic effects of fragment were detected by ELISA and platelet aggregation inhibition test, respectively.
  
  
  RESULTS
  The recovery rate of the fragment was 60.32%, its immunoactivity was 0.8 x10(-9) mol/L by ELISA. The 50% effective dose (ED(50)) in the platelet aggregate inhibition test was 3.395 mg/L.
  
  
  CONCLUSIONS
  The F(ab')(2) fragment was superior to intact IgG in binding to platelets and inhibiting platelet aggregation. Thus the F(ab')(2) fragment of mAb SZ-21 opens up broad prospects for its application to the clinical antithrombotic therapy.
authors.affiliation: Jiangsu Institute of Hematology,The First Affiliated Hospital to Suzhou University, Suzhou 215006, China.
authors.forename: Bo-Jing
authors.initials: BJ
authors.lastname: Shao
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: Zhao
authors.forename: Fei
authors.initials: F
authors.lastname: Shen
authors.forename: Chang-Geng
authors.initials: CG
authors.lastname: Ruan
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007074:Immunoglobulin G
chemical_list: D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G