Vitellogenin in rare minnow (Gobiocypris rarus): identification and induction by waterborne diethylstilbestrol.
Rare minnow (Gobiocypris rarus) is a tiny Chinese carp that has a short life cycle and is easily cultured in the laboratory. In this study, juvenile rare minnows were exposed to waterborne diethylstilbestrol (DES) at 0.05, 0.5 and 5 microg/l in laboratory aquaria. After exposure for 4, 8, 13 and 21 days, juvenile fish were collected and vitellogenin (Vtg) was measured in whole body homogenates. Native and SDS electrophoresis followed by Western blotting were performed for Vtg identification, and a non-competitive ELISA was developed. In the DES exposure groups (0.5 and 5 microg/l DES), Vtg appeared after 4 days, increased significantly after 8 days and reached a maximum on day 13. Further, a significant increase in the hepatosomatic index (HSI) was found in the 5 microg/l DES exposure group after 21 days. These results indicate that rare minnow provides a good model for assessing endocrine disruption by environmental estrogens.
Zhong
Xueping
X
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China.
Xu
Ying
Y
Liang
Yong
Y
Liao
Tao
T
Wang
Jianwei
J
D000818:Animals; D015153:Blotting, Western; D002852:Chromatography, Ion Exchange; D003530:Cyprinidae / Q000378:metabolism*; D004054:Diethylstilbestrol / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007700:Kinetics; D008297:Male; D008970:Molecular Weight; D012737:Sex Factors; D014819:Vitellogenins / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry* / Q000276:immunology; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity
D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D004054:Diethylstilbestrol