Structure of lipid A from the marine gamma-proteobacterium Marinomonas vaga ATCC 27119T lipopolysaccharide.
The chemical structure of a novel lipid A obtained as a major component on hydrolysis of LPS from the marine gamma-proteobacterium Marinomonas vaga ATCC 27119T with 1% AcOH was determined. Using chemical analysis and NMR data, it was shown to be beta-1,6-glucosaminobiose 1-phosphate acylated with R-3-hydroxydecanoic acid (at position 3), and R-3-dodecanoyloxydecanoic (or R-3-decanoyloxydecanoic) acid and R-3-(R-3-hydroxydecanoyl)oxydecanoic acids (at the 2- or 2;-positions). The absence of a fatty acid at the 3;-position and a phosphoryl group at the 4;-position, and also the presence of R-3-acyloxyalkanoic acid with R-3-hydroxyalkanoic acid as the secondary acid are unique features distinguishing the M. vaga lipid A from other ones.
Kapustina
N V
NV
Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok 690022, Russia.
Krasikova
I N
IN
Isakov
V V
VV
Gorshkova
N M
NM
Solov'eva
T F
TF
D000818:Animals; D005944:Glucosamine / Q000032:analysis; D006868:Hydrolysis; D008050:Lipid A / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000378:metabolism; D044130:Oceanospirillaceae / Q000737:chemistry*; D012623:Seawater
D008050:Lipid A; D008070:Lipopolysaccharides; D005944:Glucosamine