title: Induction of de novo subcortical actin filament assembly by Treponema denticola major outer sheath protein.
abstract: Treponema denticola and its major outer sheath protein (Msp) induce actin reorganization in fibroblasts. We adapted a barbed-end labeling/imaging assay to monitor Msp-induced subcortical actin filament assembly in neutrophils and fibroblasts. Msp, at an actin-reorganizing concentration, inhibited migration of these dissimilar cell types, whose cytoskeletal functions in locomotion and phagocytosis are crucial for immunity and healing of peripheral infections.
authors.affiliation: CHIR Group in Matrix Dynamics and Dental Research Institute, University of Toronto, Toronto, Ontario, Canada M5G 1G6.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Amin
authors.forename: Andy C S
authors.initials: AC
authors.lastname: Ho
authors.forename: Jenny Y
authors.initials: JY
authors.lastname: Lin
authors.forename: Andre Paes
authors.initials: AP
authors.lastname: Batista da Silva
authors.forename: Michael
authors.initials: M
authors.lastname: Glogauer
authors.forename: Richard P
authors.initials: RP
authors.lastname: Ellen
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism / Q000494:pharmacology*; D002465:Cell Movement; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence; D009504:Neutrophils / Q000378:metabolism*; D018272:Porins / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D014208:Treponema / Q000378:metabolism / Q000472:pathogenicity*
chemical_list: D001426:Bacterial Proteins; D018272:Porins; C099361:major outer sheath protein, Treponema