[Effect of granulocyte colony-stimulating factor on the ratio of peripheral blood dendritic cell subsets].
OBJECTIVE
To clarify the effect of granulocyte colony-stimulating factor (G-CSF) on the radio of two peripheral blood dendritic cell(DC) subsets in-vivo.


METHODS
Various doses (0, 5, 10 and 15 microg) of G-CSF were subcutaneosly injected respectively into BALB/c mice, once each day, for 6 days running. Six days later, DCs were separated from peripheral blood. Then the flow cytometry was used to detect the radio of DC1 and DC2 (CD11c(+) CD8a(-) and CD11c(+) CD8a(+)), and to calculate their absolute numbers.


RESULTS
After stimulation with varying doses of G-CSF for 6 days, the absolute number of DC2s increased from 9.6x10(6)/L to 55.1x10(6)/L (P<0.01), while that of DC1s had no notably variation, so the ratio of DC1 and DC2 decreases from 4.2+/-1.1 to 0.7+/-0.3 (P<0.01).


CONCLUSIONS
G-CSF can increase the absolute number of peripheral blood DC2s, but has no influence on peripheral blood DC1 number, thus inversing the ratio of DC1/DC2 in-vivo.
Zhou
Jing-Shi
JS
Department of Hepato-biliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Dou
Ke-Feng
KF
Sun
Kai
K
Fu
Rong
R
Li
Hai-Min
HM
Zhang
Fu-Qin
FQ
D000818:Animals; D001773:Blood Cells; D003713:Dendritic Cells / Q000276:immunology; D005434:Flow Cytometry; D016179:Granulocyte Colony-Stimulating Factor
D016179:Granulocyte Colony-Stimulating Factor