[Functional interaction between nicotinic cholinergic receptors and Na, K-ATPase in the skeletal muscles].
Acetylcholine (ACh) hyperpolarized the rat diaphragm muscle fibers by 4.5 +/- 0.8 mV (K0.5 = = 36 +/- 6 nmol/l). The AC-induced hyperpolarization was blocked by d-tubocurarine and ouabain in nanomolar concentrations. This effect of ACh was not observed in cultured C2C12 muscle cells and in Xenopus oocytes with expressed embryonic mouse muscle nicotinic acetylcholine receptors (nAChR) or with neuronal alpha 4 beta 2 nAChR. In membrane preparations from the Torpedo californica electric organ, containing both nAChR and Na, K-ATPase, 10 nmol/l ouabain modulated the binding kinetics of the cholinergic ligand dansyl-C6-choline to the nAChR. These results suggest that in-sensitive alpha 2 isoform) and nAChR in a state with high affinity to Ach and d-tubocurarine may form a functional complex in which binding of ACh to nAchR is coupled to activation of the Na, K-ATPase.
Krivoĭ
I I
II
St. Petersburg State University, 199034, St. Petersburg, University Emb., 7/9, Russia.
Drabkina
T M
TM
Dobretsov
M G
MG
Vasil'ev
A N
AN
Kravtsova
V V
VV
Eaton
M J
MJ
Skachkov
S N
SN
Mandel
F
F
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D018678:Cholinergic Agents / Q000494:pharmacology; D003964:Diaphragm / Q000187:drug effects / Q000502:physiology; D004557:Electric Organ / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D066298:In Vitro Techniques; D008024:Ligands; D008564:Membrane Potentials / Q000187:drug effects; D008566:Membranes / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000502:physiology; D018482:Muscle, Skeletal / Q000187:drug effects / Q000502:physiology*; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D011978:Receptors, Nicotinic / Q000187:drug effects / Q000502:physiology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000502:physiology*; D014101:Torpedo; D014981:Xenopus
D018678:Cholinergic Agents; D008024:Ligands; D011978:Receptors, Nicotinic; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase; D000109:Acetylcholine