title: Fibroblast growth-stimulating activity of S100A9 (MRP-14).
abstract: Fibroblasts play a critical role in chronic inflammation and wound healing. In this study, a fibroblast growth-stimulating factor was purified from the exudate of carrageenan-induced inflammation in rats. The purified protein was a disulfide-linked homodimer. Amino acid sequence analysis of the peptides generated by cleavage with cyanogen bromide and proteinase V8 resulted in identification of the protein as S100A9. Recombinant S100A9 as well as its disulfide-linked homodimer stimulated the proliferation of fibroblasts at a similar concentration of the purified protein. The concentration of S100A9 in the exudate was determined by immunoblot analysis. The total protein concentration in the exudate reached a maximum 4 days after carrageenan injection and then slightly decreased, whereas the concentration of S100A9 reached a maximum at day 3 and then decreased rapidly. These studies show that S100A9 is present at a high concentration in the exudate of carrageenan-induced inflammation in rats, and that S100A9 stimulates proliferation of fibroblasts, suggesting that it plays a role in chronic inflammation.
authors.affiliation: Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan. fshibata@ms.toyama-mpu.ac.jp
authors.forename: Futoshi
authors.initials: F
authors.lastname: Shibata
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Miyama
authors.forename: Fumie
authors.initials: F
authors.lastname: Shinoda
authors.forename: Jun
authors.initials: J
authors.lastname: Mizumoto
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Takano
authors.forename: Hideo
authors.initials: H
authors.lastname: Nakagawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D040502:Calgranulin B / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D002478:Cells, Cultured; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D007249:Inflammation / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis
chemical_list: D040502:Calgranulin B; D011994:Recombinant Proteins; D005346:Fibroblast Growth Factors