title: [Effects of LFA-1 costimulation on proliferation and IgG production of PBMCs from lupus nephritis patients].
abstract: OBJECTIVE
  To study the effect of lymphocyte function associated antigen-1(LFA-1) costimulation on proliferation and immunoglobin production of peripheral blood mononuclear cells(PBMCs) form lupus nephritis(LN) patients.
  
  
  METHODS
  29 LN patients were enrolled in this study, and 12 healthy persons served as control. PBMCs from LN patients and healthy persons were obtained by Ficoll density gradient centrifugation, and cell proliferation was detected by (3)H-TdR incorporation. IgG content in cultural supermatant was detected by ELISA.
  
  
  RESULTS
  Stimulation of anti-CD3 mAb alone could enhance the proliferation and IgG production of PBMCs from 29 LN patients,while the effects on PBMCs from patients in active phase were stronger than those from the patients in the inactive phase (P<0.01). But anti-CD3 mAb had no influenence on PBMCs from healthy persons.The costimulation of anti-CD3 mAb and LFA-1 could increase proliferation and IgG production of PBMCs from LN patients and healthy persons. The effects of this costimulation decreased in turn from active and inactive LN patients to normal persons (P<0.01). The costimulant effects of LFA-1 was inhibited by anti-LFA-1 mAb.
  
  
  CONCLUSIONS
  The effects of enhancing PBMC proliferation and IgG production by LFA-1 may be a mechanism of LN pathogenesis.
authors.affiliation: Department of Nephrology, Sun Yat-sen University of Medical Sciences, Guangzhou 510080,China.
authors.forename: Jian-Qin
authors.initials: JQ
authors.lastname: Wang
authors.forename: You-Ji
authors.initials: YJ
authors.lastname: Li
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Xia
authors.forename: Ren-Gao
authors.initials: RG
authors.lastname: Ye
authors.forename: Di-Hua
authors.initials: DH
authors.lastname: Zhang
authors.forename: Dao-Yuan
authors.initials: DY
authors.lastname: Zhou
mesh_terms: D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008181:Lupus Nephritis; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1
chemical_list: D007074:Immunoglobulin G; D007376:Interleukin-2; D016169:Lymphocyte Function-Associated Antigen-1