Protective effect of interleukin-1beta on motor neurons after sciatic nerve injury in rats.
Protective effect of interleukin-1beta (IL-1beta) on motor neurons was studied after peripheral nerve injury. Twenty Wistar rats were divided into 2 groups randomly. The right sciatic nerve of each rat was resected. After silicon tubulization of sciatic nerve in rat, 15 microl 1 ng/ml IL-1beta and PBS solution were injected into the silicon capsule respectively. Enzyme histochemistry was performed to show acetyle cholesterase (AchE) and nitric oxide staining (NOS) activity of spinal alpha motor neurons in spinal segments 2 weeks later. Neurons were counted and the diameter and cross sectional (c/s) area of neurons were analyzed by using computer image analysis system. The results showed that as compared with the normal side, both enzyme activities significantly changed in motor neurons in PBS group. The diameter and c/s area of both neurons changed significantly too (P < 0.01). These results suggest that exogenous IL-1beta protects alpha-motor neurons from degeneration and necrosis after peripheral nerve injury.
Weng
Yuxiong
Y
Department of Hand Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.
Khatri
Bharat
B
Hong
Guangxiang
G
Wang
Fabin
F
Chen
Zhenbin
Z
Huang
Qishun
Q
D000818:Animals; D005260:Female; D007375:Interleukin-1 / Q000494:pharmacology*; D008297:Male; D009046:Motor Neurons / Q000473:pathology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000293:injuries*; D013116:Spinal Cord / Q000473:pathology
D007375:Interleukin-1; D018696:Neuroprotective Agents