Acetazolamide inhibits aquaporin-1 protein expression and angiogenesis.
OBJECTIVE
To study effects of acetazolamide on aquaporin-1 (AQP(1)) protein expression and angiogenesis.


METHODS
Establishing Lewis-lung-carcinoma model, the localization of AQP(1) in tumor tissues was investigated by immunohistochemical methods; The biological activity of acetazolamide was detected by endothelial cells proliferation test (MTT) assay and chorioallantoic membrane (CAM) vascular inhibition test.


RESULTS
Immunohistochemical localization of AQP(1) in mice tumor was labeled in capillaries, post capillary venules endothelial cells. After being treated with acetazolamide, the number of capillaries and post capillary venules was significantly decreased in tumor tissue. Acetazolamide showed significant inhibitory effect on angiogenesis in CAM and endothelial cell proliferation.


CONCLUSIONS
Acetazolamide might be identified and developed as one of potential lead compounds for a new therapeutic intervention in inhibiting cancer angiogenesis.
Xiang
Yang
Y
Department of Pharmacology, School of Basic Medical Sciences, Peking University, Beijing 100083, China.
Ma
Bing
B
Li
Tao
T
Gao
Jun-Wei
JW
Yu
He-Ming
HM
Li
Xue-Jun
XJ
D000086:Acetazolamide / Q000494:pharmacology*; D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D051398:Aquaporin 1; D020346:Aquaporins / Q000378:metabolism*; D001789:Blood Group Antigens; D002196:Capillaries / Q000378:metabolism; D018827:Carcinoma, Lewis Lung / Q000098:blood supply / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002642:Chick Embryo; D002823:Chorion / Q000098:blood supply; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D005260:Female; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic
C496020:AQP1 protein, human; D020533:Angiogenesis Inhibitors; C496021:Aqp1 protein, mouse; D020346:Aquaporins; D001789:Blood Group Antigens; D051398:Aquaporin 1; D000086:Acetazolamide