title: Methyl malondialdehyde is not suitable as an internal standard for malondialdehyde detection in urine after derivatisation with 2,4-dinitrophenylhydrazine.
abstract: A previously described method of measurement of malondialdehyde (MDA) in human urine after derivatisation with 2,4-dinitrophenylhydrazine (DNPH) was tested for a possibility of using methyl malondialdehyde (MeMDA) as an internal standard. Despite structural similarity, those compounds were found to produce different yields of derivatisation under the same conditions depending on urine matrix. We conclude, that MeMDA is not suitable as an internal standard for the measurement of MDA in urine under previously reported conditions when DNPH is used as a deriviatising agent.
authors.affiliation: Institute of Science and Technology in Medicine, School of Medicine, University of Keele, Thornburrow Drive, Hartshill, Stoke-on-Trent, ST4 7QB, UK. o.v.exley@keele.ac.uk
authors.forename: Olga
authors.initials: O
authors.lastname: Korchazhkina
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry*; D008315:Malondialdehyde / Q000031:analogs & derivatives* / Q000652:urine*; D010659:Phenylhydrazines / Q000737:chemistry*; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007202:Indicators and Reagents; D010659:Phenylhydrazines; C033319:methylmalondialdehyde; C004787:2,4-dinitrophenylhydrazine; D008315:Malondialdehyde