[Effect of antisense CD40 RNA on CD40 expression and proliferation of EB virus-transformed B lymphocytes].
OBJECTIVE
To investigate the changes of CD40 expression and proliferation pattern of EB virus-transformed B lymphocytes that had been transfected by antisense CD40 RNA.


METHODS
Eukaryotic expression vector pcDNA3/CD40 of human CD40antisense RNA was constructed by means of T-A cloning and subcloning, and then was transfected into EB virus-transformed B lymphocytes. Changes of CD40 expression on the B cells and their proliferation were tested by flow cytometry and MTT colorimetry assay, respectively.


RESULTS
CD40 expression and proliferation of the B cells transfected with antisense CD40 RNA were significantly lower than those of the cells transfected with pcDNA3 alone (P < 0. 01).


CONCLUSIONS
Antisense CD40 RNA may be served as an effective means of regulating immune function, and the CD40 gene itself also play an important role in cell proliferation.
Zheng
Xiang-xiong
XX
Institute of Clinical Immunology, Union Hospital Affiliated to Fujian Medical University, Fuzhou 350001, China. Zhengx@pub1.fz.fj.cn
Li
He-jun
HJ
Li
Pin
P
Zhou
Xiao-ling
XL
D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000821:virology; D019013:CD40 Antigens / Q000235:genetics*; D002461:Cell Line, Transformed; D004854:Herpesvirus 4, Human; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D016372:RNA, Antisense / Q000494:pharmacology*
D019013:CD40 Antigens; D016372:RNA, Antisense