title: Human ACAT-1 and -2 inhibitory activities of saucerneol B, manassantin A and B isolated from Saururus chinensis.
abstract: The sesquineolignan, saucerneol B (1), and dineolignans, manassantin A (2), and manassantin B (3), were isolated from the methanol extracts of Saururus chinensis root and elucidated by their spectroscopic data analysis. Compounds 1-3 inhibited hACAT-1 and hACAT-2 with IC(50) values of 43.0 and 124.0 microM for 1, of 39.0 and 8.0 microM for 2, of 82.0 microM and only 32% inhibition at 1mM for 3, respectively. The EtOAc-soluble fraction, which contained compounds 1-3, of methanol extracts of S. chinensis exhibited strong cholesterol-lowering effect in high cholesterol-fed mice.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, 52 Oun, Yusong, Daejeon 305-333, Republic of Korea.
authors.forename: Woo Song
authors.initials: WS
authors.lastname: Lee
authors.forename: Dae-Woo
authors.initials: DW
authors.lastname: Lee
authors.forename: Young-Il
authors.initials: YI
authors.lastname: Baek
authors.forename: Sojin
authors.initials: S
authors.lastname: An
authors.forename: So-Jin
authors.initials: SJ
authors.lastname: An
authors.forename: Kyung-Hyun
authors.initials: KH
authors.lastname: Cho
authors.forename: Yang-Kyu
authors.initials: YK
authors.lastname: Choi
authors.forename: Hyoung-Chin
authors.initials: HC
authors.lastname: Kim
authors.forename: Ho-Yong
authors.initials: HY
authors.lastname: Park
authors.forename: Ki-Hwan
authors.initials: KH
authors.lastname: Bae
authors.forename: Tae-Sook
authors.initials: TS
authors.lastname: Jeong
mesh_terms: D000818:Animals; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005663:Furans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots; D032134:Saururaceae; D002785:Sterol O-Acyltransferase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000097587:Sterol O-Acyltransferase 2
chemical_list: D004791:Enzyme Inhibitors; D005663:Furans; D017705:Lignans; D010936:Plant Extracts; D002785:Sterol O-Acyltransferase; D000097587:Sterol O-Acyltransferase 2; C482885:manassantin B; C446969:saucerneol B; C054895:manassantin A; C493492:sterol O-acyltransferase 1