[Effects of the escharectomy during burn shock stage on expression of glucose translator-4 mRNA in skeletal muscle and adipose tissue].
OBJECTIVE
To investigate the effect of the escharectomy during burn shock stage on expression of glucose translator-4 (GLUT4) mRNA in skeletal muscle and adipose tissue.


METHODS
30% TBSA scalded rats were employed. Escharectomy were conducted at 8 h, 24 h, 168 h after burns respectively. Insulin, glucagon, cortisol and glucose levels in serum were analyzed. RT-PCR were employed to analyze GLUT4 mRNA expression in skeletal muscle and adipose tissue.


RESULTS
Glucagon, cortisol and glucose levels in serum were declined in groups which escharectomy were conducted during burn shock stage. GLUT4 mRNA expression in both skeletal muscle and adipose tissue were downregulated after burns and escharectomy conducted during burn shock stage made it restored to near normal.


CONCLUSIONS
GLUT4 mRNA expression will declined after major burns in skeletal muscle and adipose tissue. Escharectomy during shock stage could make it upregulated, which will be helpful to improve glucose metabolism and hypermetabolism after major burns.
Shuai
Xiu-rong
XR
Burns department, Hospital of Armed Police in Sichuan, Leshan 614000, China.
Liu
Tong-fa
TF
Guo
Zhen-rong
ZR
Yu
Shun-xian
SX
He
Peng-fei
PF
Yuan
Wen-zhou
WZ
Li
Feng
F
He
Li-xin
LX
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose; D002056:Burns / Q000503:physiopathology / Q000601:surgery*; D015870:Gene Expression; D005934:Glucagon / Q000097:blood; D006854:Hydrocortisone / Q000097:blood; D007328:Insulin / Q000097:blood; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D018482:Muscle, Skeletal / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012774:Shock, Traumatic / Q000503:physiopathology
D001786:Blood Glucose; D007328:Insulin; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; D005934:Glucagon; D006854:Hydrocortisone