title: Primitive complement system--recognition and activation.
abstract: The complement system, composed of more than 30 serum and cell surface components, is collaborating in recognition and elimination of pathogens as a part of both the innate and acquired immune systems. The two collagenous lectins, mannose-binding lectin (MBL) and ficolins, are one of the pattern recognition molecules acting in innate immunity and upon recognition of the pathogens, they trigger the activation of the lectin complement pathway through attached serine proteases (MASPs). A similar lectin-base complement system, consisting of the lectin-protease complex and C3, is present in ascidians, our closest invertebrate relatives and functions in an opsonic manner. On the other hand, ongoing genome projects in both vertebrates and invertebrates revealed that most domains used by mammalian complement components are found in both protostomes and deuterostomes. However, the unique combinations of them as found in mammalian complement components are present only in deuterostomes, indicating the deuterostome origin of the complement system. Unexpectedly, the complement system of an invertebrate deuterostome, ascidian, shows a similar level of complexity as that of mammals, suggesting that expansion of complement genes by gene duplications occurred independently both in the ascidian and vertebrate lineages. Although most characteristic domain structures of the mammalian complement components are found in ascidians, detailed evolutionary analysis casts doubt on their mutual reactivity in several points. Thus, another integrative step seems to have been required to establish the modern complement system of higher vertebrates.
authors.affiliation: Department of Biochemistry, Fukushima Medical University, and CREST, Japan Science and Technology Agency, Japan. tfujita@fmu.ac.jp
authors.forename: Teizo
authors.initials: T
authors.lastname: Fujita
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Endo
authors.forename: Masaru
authors.initials: M
authors.lastname: Nonaka
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000276:immunology; D003167:Complement Activation; D003165:Complement System Proteins / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007113:Immunity, Innate; D037102:Lectins; D037601:Mannose-Binding Lectin / Q000276:immunology; D050606:Mannose-Binding Protein-Associated Serine Proteases; D012697:Serine Endopeptidases / Q000276:immunology; D000097604:Ficolins
chemical_list: D002352:Carrier Proteins; D003165:Complement System Proteins; D037102:Lectins; D037601:Mannose-Binding Lectin; D050606:Mannose-Binding Protein-Associated Serine Proteases; D012697:Serine Endopeptidases; D000097604:Ficolins