Delivery of the immunosuppressive antigen Salp15 to antigen-presenting cells by Salmonella enterica serovar Typhimurium aroA mutants.
A Salmonella enterica serovar Typhimurium aroA-deficient delivery system was used to target the immunosuppressive protein Salp15 to antigen-presenting cells. In vitro and in vivo infections with Salp15-containing Salmonella resulted in an impaired CD4(+)-T-cell activation, suggesting that the protein was produced by antigen-presenting cells in a physiologically active form.
Motameni
Amir-Reza T
AR
Department of Biology, University of North Carolina at Charlotte, Charlotte, North Carolina 28223, USA.
Juncadella
Ignacio J
IJ
Ananthanarayanan
Shobana K
SK
Hedrick
Michael N
MN
Huet-Hudson
Yvette
Y
Anguita
Juan
J
D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D019883:Alkyl and Aryl Transferases / Q000235:genetics; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000473:pathology; D002478:Cells, Cultured; D005822:Genetic Vectors; D008213:Lymphocyte Activation / Q000502:physiology; D016676:Macrophages, Alveolar; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D012471:Salivary Proteins and Peptides / Q000235:genetics / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000235:genetics / Q000472:pathogenicity*
D012471:Salivary Proteins and Peptides; C464566:Salp15 protein, Ixodes scapularis; D019883:Alkyl and Aryl Transferases; D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase