Anaerobranca californiensis sp. nov., an anaerobic, alkalithermophilic, fermentative bacterium isolated from a hot spring on Mono Lake.
A novel, obligately anaerobic, alkalithermophilic, chemo-organotrophic bacterium was isolated from the sediment of an alkaline hot spring located on Paoha Island in Mono Lake, California, USA. This rod-shaped bacterium was motile via peritrichous flagella. Isolated strains grew optimally in 5-25 g NaCl l(-1), at pH 9.0-9.5 and at a temperature of 58 degrees C and were fermentative and mainly proteolytic, utilizing peptone, Casamino acids and yeast extract. Optimal growth was seen in the presence of elemental sulfur, polysulfide or thiosulfate with concomitant reduction to hydrogen sulfide. Sulfite was also formed in an equal ratio to sulfide during reduction of thiosulfate. The novel isolate could also reduce Fe(III) and Se(IV) in the presence of organic matter. On the basis of physiological properties, 16S rRNA gene sequence and DNA-DNA hybridization data, strain PAOHA-1(T) (=DSM 14826(T)=UNIQEM 227(T)) belongs to the genus Anaerobranca and represents a novel species, Anaerobranca californiensis sp. nov.
Gorlenko
Vladimir
V
Institute of Microbiology, Russian Academy of Science, Moscow 117811, Russia.
Tsapin
Alexandre
A
University of Southern California, Los Angeles, CA 90089, USA.|Jet Propulsion Laboratory/California Institute of Technology, Pasadena, CA 91109-8099, USA.
Namsaraev
Zorigto
Z
Institute of Microbiology, Russian Academy of Science, Moscow 117811, Russia.
Teal
Tracy
T
California Institute of Technology, Pasadena, CA 91125, USA.
Tourova
Tatyana
T
Institute of Microbiology, Russian Academy of Science, Moscow 117811, Russia.
Engler
Diane
D
Jet Propulsion Laboratory/California Institute of Technology, Pasadena, CA 91109-8099, USA.
Mielke
Randy
R
Jet Propulsion Laboratory/California Institute of Technology, Pasadena, CA 91109-8099, USA.
Nealson
Kenneth
K
University of Southern California, Los Angeles, CA 90089, USA.|Jet Propulsion Laboratory/California Institute of Technology, Pasadena, CA 91109-8099, USA.
D001421:Bacteria, Anaerobic / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002140:California; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005285:Fermentation; D005618:Fresh Water / Q000382:microbiology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013440:Sulfides / Q000378:metabolism
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013440:Sulfides