Induction of de novo subcortical actin filament assembly by Treponema denticola major outer sheath protein.
Treponema denticola and its major outer sheath protein (Msp) induce actin reorganization in fibroblasts. We adapted a barbed-end labeling/imaging assay to monitor Msp-induced subcortical actin filament assembly in neutrophils and fibroblasts. Msp, at an actin-reorganizing concentration, inhibited migration of these dissimilar cell types, whose cytoskeletal functions in locomotion and phagocytosis are crucial for immunity and healing of peripheral infections.
Amin
Mohsen
M
CHIR Group in Matrix Dynamics and Dental Research Institute, University of Toronto, Toronto, Ontario, Canada M5G 1G6.
Ho
Andy C S
AC
Lin
Jenny Y
JY
Batista da Silva
Andre Paes
AP
Glogauer
Michael
M
Ellen
Richard P
RP
D008841:Actin Cytoskeleton / Q000378:metabolism*; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism / Q000494:pharmacology*; D002465:Cell Movement; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence; D009504:Neutrophils / Q000378:metabolism*; D018272:Porins / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D014208:Treponema / Q000378:metabolism / Q000472:pathogenicity*
D001426:Bacterial Proteins; D018272:Porins; C099361:major outer sheath protein, Treponema