title: Transcriptional co-activator activity of SYT is negatively regulated by BRM and Brg1.
abstract: The t(X;18)(p11.2;q11.2) translocation found in synovial sarcomas results in the fusion of the SYT gene on chromosome 18 to the SSX gene on chromosome X. Although the SYT-SSX fusion proteins may trigger synovial sarcoma development, the biological functions of SYT, SSX and SYT-SSX genes are unclear. Transfections of Gal4 DNA binding domain fusion protein constructs demonstrate that SYT protein acts as a transcriptional co-activator at the C-terminal domain and that the activity is repressed through the N-terminus. The N-terminal 70 amino acids of SYT bind not only to BRM, but also to Brg1, both of which are subunits of SWI/SNF chromatin remodelling complexes. Here, we have investigated the functions of BRM and Brg1 on the repression of SYT activity. The negative regulation of SYT transcriptional co-activator activity is dependent on the ATP-hydrolysis of BRM and Brg1 in the protein complexes. This indicates that the SWI/SNF protein complexes regulate SYT activity using the chromatin remodelling activity.
authors.affiliation: Center for Medical Genomics, National Cancer Center Research Institute, 5-1-1 Tsukiji Chuo-ku, Tokyo 104-0045, Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Ishida
authors.forename: Shinya
authors.initials: S
authors.lastname: Tanaka
authors.forename: Misao
authors.initials: M
authors.lastname: Ohki
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Ohta
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D002460:Cell Line; D042002:Chromatin Assembly and Disassembly; D002868:Chromosomal Proteins, Non-Histone / Q000502:physiology; D004265:DNA Helicases; D006801:Humans; D006868:Hydrolysis; D009687:Nuclear Proteins / Q000502:physiology*; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000502:physiology*; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology*; D015533:Transcriptional Activation
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; C084011:SMARCA2 protein, human; C093961:SS18 protein, human; C445179:SWI-SNF-B chromatin-remodeling complex; D014157:Transcription Factors; D000255:Adenosine Triphosphate; C084108:SMARCA4 protein, human; D004265:DNA Helicases