title: Live cold-adapted influenza A vaccine produced in Vero cell line.
abstract: The African green monkey kidney (Vero) cell line was used as a substrate for the development of a live cold-adapted (ca) reassortant influenza vaccine. For that purpose, a new master strain was generated by an adaptation of the wild type (wt) A/Singapore/1/57 virus to growth at 25 degrees C in a Vero cell line. The resulting cold-adapted (ca) muster strain A/Singapore/1/57ca showed temperature sensitive (ts) phenotype and was attenuated in animal models and protective in the challenge experiments in ferrets. Two vaccine candidates of influenza A(H1N1) and A(H3N2) subtypes (6/2 reassortants) inheriting six genes coding internal proteins from the new master strain and the surface antigens hemagglutinin (HA) and neuraminidase (NA) from the epidemic viruses were obtained by a standard method of genetic reassortment. All steps of the vaccine preparation were done exclusively in Vero cells, including the isolation of the epidemic viruses. Both vaccine strains were used for immunization of young adult volunteers in a limited clinical trial and appeared to be safe, well tolerated and immunogenic after intranasal administration.
authors.affiliation: Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18B, A-1190 Vienna, Austria. j.romanova@iam.boku.ac.at
authors.forename: Julia
authors.initials: J
authors.lastname: Romanova
authors.forename: Dietmar
authors.initials: D
authors.lastname: Katinger
authors.forename: Boris
authors.initials: B
authors.lastname: Ferko
authors.forename: Brigitta
authors.initials: B
authors.lastname: Vcelar
authors.forename: Sabine
authors.initials: S
authors.lastname: Sereinig
authors.forename: Oleg
authors.initials: O
authors.lastname: Kuznetsov
authors.forename: Marina
authors.initials: M
authors.lastname: Stukova
authors.forename: Marjana
authors.initials: M
authors.lastname: Erofeeva
authors.forename: Oleg
authors.initials: O
authors.lastname: Kiselev
authors.forename: Hermann
authors.initials: H
authors.lastname: Katinger
authors.forename: Andrej
authors.initials: A
authors.lastname: Egorov
mesh_terms: D000222:Adaptation, Physiological; D000328:Adult; D000818:Animals; D002522:Chlorocebus aethiops; D003080:Cold Temperature; D005289:Ferrets; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology; D007251:Influenza, Human / Q000517:prevention & control; D051379:Mice; D008807:Mice, Inbred BALB C; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology; D014709:Vero Cells / Q000821:virology; D014779:Virus Replication
chemical_list: D007252:Influenza Vaccines; D014613:Vaccines, Attenuated