title: Association of PTEN mutation with HPV-negative adenocarcinoma of the uterine cervix.
abstract: Serous, mucinous, endometrioid, and clear cell adenocarcinomas arise from reproductive organs of mullerian origin. Although the mutation of PTEN, a tumor suppressor, is known to be involved in tumorigenesis of endometrioid adenocarcinomas of the endometrium and ovary, the role of PTEN alteration in endometrioid adenocarcinoma of the cervix remains to be investigated. To elucidate the molecular pathogenesis of cervical adenocarcinoma and adenosquamous carcinoma, and in particular to examine the potential role of PTEN mutation in endometrioid-type cancer of the cervix, we analyzed 32 cervical adeno- or adenosquamous carcinomas (8 endometrioid adenocarcinomas, 14 mucinous adenocarcinomas and 10 adenosquamous carcinomas) for PTEN mutations and HPV infections. PTEN mutation was detected in 2 of 8 (25.0%) endometrioid cases, 2 of 14 (14.3%) mucinous cases, and none of 10 (0%) adenosquamous cases. HPV DNA was detected in 11 out of 18 (61.1%) PTEN wild-type adenocarcinomas and 8 out of 10 (80.0%) adenosquamous carcinomas. Among 11 HPV-negative adenocarcinomas, 40.0% (2/5) endometrioid cases and 33.3% (2/6) mucinous cases were shown to be PTEN mutated, while no cases (0/21) were PTEN-mutant in the remainder (i.e. adenosquamous carcinomas and HPV-positive adenocarcinomas). The current observations suggest that PTEN mutation is frequently detected in HPV-negative adenocarcinomas of the cervix and the most prevalent occurrence of PTEN mutation in endometrioid subtype is keeping with endometrial and ovarian carcinomas.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Tokyo 113-8655, Japan.
authors.forename: Takeo
authors.initials: T
authors.lastname: Minaguchi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yoshikawa
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Nakagawa
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Yasugi
authors.forename: Tetsu
authors.initials: T
authors.lastname: Yano
authors.forename: Haruko
authors.initials: H
authors.lastname: Iwase
authors.forename: Katsumi
authors.initials: K
authors.lastname: Mizutani
authors.forename: Kenji
authors.initials: K
authors.lastname: Shiromizu
authors.forename: Kazuo
authors.initials: K
authors.lastname: Ohmi
authors.forename: Yoh
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Kiichiro
authors.initials: K
authors.lastname: Noda
authors.forename: Mieko
authors.initials: M
authors.lastname: Nishiu
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Taketani
mesh_terms: D002288:Adenocarcinoma, Mucinous / Q000235:genetics* / Q000821:virology; D000328:Adult; D000368:Aged; D018196:Carcinoma, Adenosquamous / Q000235:genetics / Q000821:virology; D018269:Carcinoma, Endometrioid / Q000235:genetics* / Q000821:virology; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D051059:PTEN Phosphohydrolase; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000235:genetics / Q000821:virology; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000821:virology
chemical_list: D004279:DNA, Viral; D025521:Tumor Suppressor Proteins; D010744:Phosphoric Monoester Hydrolases; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human