An approach to a FMD vaccine based on genetic engineered attenuated pseudorabies virus: one experiment using VP1 gene alone generates an antibody responds on FMD and pseudorabies in swine.
Foot-and-mouth disease (FMD) and pseudorabies (PR) are two important infectious diseases in swine. An attenuated pseudorabies virus (PRV) has been successfully used as a gene delivery vector for the development of live-viral vaccines. In this study, a recombinant PRV-VP1 virus was constructed by fusioning the VP1 gene of FMD virus in frame to the N-terminal sequence of the gG gene of PRV. To test the protective immunity, 15 FMDV sero-negative white swine were divided into three groups and immunized with the recombinant PRV-VP1 virus, commercial FMD vaccine and vector virus (TK(-)/gG(-)/LacZ(+)), respectively, and challenged intramuscularly with 20 minimal infecting doses (MID) of virulent type O FMDV 4 weeks after booster immunization. Swine vaccinated with PRV-VP1 acquired antibodies against both FMDV and PRV, however, anti-FMDV antibodies were much lower than those vaccinated with the commercial FMD vaccine. Our results suggested that the recombinant PRV-VP1 virus, which only expressed FMDV VP1 gene controlled by PRV gG promoter, could not protect swine from the challenge of 20 MID type O FMDV, but could delay and reduce the clinical symptoms of FMD.
Qian
Ping
P
Laboratory of Animal Virology, College of Veterinary Medical Sciences, Huazhong Agricultural University, Wuhan 430070, PR China.
Li
Xiang-Min
XM
Jin
Mei-Lin
ML
Peng
Gui-Qing
GQ
Chen
Huan-Chun
HC
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D036022:Capsid Proteins / Q000235:genetics* / Q000276:immunology; D005334:Fever / Q000662:veterinary; D005528:Foot / Q000473:pathology; D005536:Foot-and-Mouth Disease / Q000503:physiopathology / Q000517:prevention & control*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology*; D011558:Herpesvirus 1, Suid / Q000235:genetics* / Q000276:immunology; D009500:Neutralization Tests; D011557:Pseudorabies / Q000517:prevention & control; D022421:Pseudorabies Vaccines / Q000235:genetics / Q000276:immunology*; D013552:Swine / Q000276:immunology / Q000821:virology; D013553:Swine Diseases / Q000503:physiopathology / Q000517:prevention & control; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D036022:Capsid Proteins; D022421:Pseudorabies Vaccines; C442501:VP1 protein, Foot-and-mouth disease virus; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic; D014765:Viral Vaccines