title: A Technique for Automated Quantification of Canine Glucocorticoid-induced Isoenzyme of Alkaline Phosphatase.
abstract: A sensitive assay for the corticosteroid-induced alkaline phosphatase isoenzyme (CAP), adaptable to most clinical chemistry autoanalyzers, is described and validated. This assay is based on the greater than 98% inhibition of liver alkaline phosphatase isoenzyme (LAP) activity with 4.2 mM levamisole, as compared to the 42% inhibition of CAP activity. Analysis of serum with total alkaline phosphatase (AP) activity within the reference range, resulted in a reference range of 0 to 19 U/L for CAP activity. Analysis of serum from 160 clinical patients with AP activity above the reference range, revealed 73% with increased CAP activity ranging from 20 to 7,000 U/L. The diagnostic significance of this increased CAP activity is discussed.
authors.forename: W E
authors.initials: WE
authors.lastname: Hoffmann
authors.forename: R K
authors.initials: RK
authors.lastname: Sanecki
authors.forename: J L
authors.initials: JL
authors.lastname: Dorner