Murine plasmacytoid dendritic cells induce effector/memory CD8+ T-cell responses in vivo after viral stimulation.
Like their human counterparts, mouse plasmacytoid dendritic cells (pDCs) play a central role in innate immunity against viral infections, but their capacity to prime T cells in vivo remains unknown. We show here that virus-activated pDCs differentiate into antigen-presenting cells able to induce effector/memory CD8(+) T-cell responses in vivo against both epitopic peptides and endogenous antigen, whereas pDCs activated by synthetic oligodeoxynucleotides containing unmethylated cytosine-guanine motifs (CpG) acquire only the ability to recall antigen-experienced T-cell responses. We also show that immature pDCs are unable to induce effector or regulatory CD8(+) T-cell responses. Thus, murine pDCs take part in both innate and adaptive immune responses by directly priming naive CD8(+) T cells during viral infection.
Schlecht
Géraldine
G
Unité de Biologie des Régulations Immunitaires, Institut National de la Santé et de la Recherche Médicale, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris cedex 15, France.
Garcia
Sylvie
S
Escriou
Nicolas
N
Freitas
Antonio A
AA
Leclerc
Claude
C
Dadaglio
Gilles
G
D000818:Animals; D017951:Antigen Presentation; D000956:Antigens, Viral / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology*; D002454:Cell Differentiation; D002465:Cell Movement; D018899:CpG Islands / Q000502:physiology; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology* / Q000821:virology; D005260:Female; D006358:Hot Temperature; D007156:Immunologic Memory / Q000187:drug effects / Q000276:immunology*; D008297:Male; D051379:Mice; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D009975:Orthomyxoviridae / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology; D013154:Spleen / Q000276:immunology / Q000473:pathology
D000956:Antigens, Viral; D009838:Oligodeoxyribonucleotides; D010047:Ovalbumin