Low-frequency electromagnetic stimulation may lead to regression of Morris hepatoma in buffalo rats.
OBJECTIVE
The influence of low-frequency electromagnetic (LF-EM) waves on the processes of carcinogenesis and tumor growth has been the subject of experimental investigations for more than two decades and the results are promising. In parallel, an interesting method of complementary medicine, biophysical-information therapy (BIT) or bioresonance therapy (BRT), which in principle is based on LF-EM stimulation, has emerged. BRT has been known since the late 1980s but is still poorly studied. The idea of applying BRT to tumors is based on two main premises: (1) endogenous biophotonic emission in the case of tumors is different from that produced by healthy tissues/cells and (2) BRT effects are believed to be primarily manifested at the immune-system level. Consequently, we decided to study the influence of BRT on a dynamic and well-known process: the expansion of transplantable hepatoma in rats.


METHODS
The study was carried out on female Buffalo rats with implanted Morris tumors (three experimental and one control group). Fourteen (14) consecutive in vivo exposures using a BRT device (BICOM B15, REGUMED Regulative Medizintechnik Gmbh, Graefelfing, Germany), were made from the third day after inoculation of the tumors. Biometric observations, intra vitam (tumor volume, growth ratio), were completed with histologic investigation (implantation locus, selected internal organs [lungs]).


RESULTS
Thirty-one (31) cases (69%; n = 45) of total tumor regression were observed in experimental groups and these individuals were anesthetized to enable histologic verification to be made. No lung metastases--usually observed in tumor-bearers--could be detected. Moreover, in the inoculation loci, traces of former implantation and tumor absorption were found to be associated with high activity of cell-mediated immune response. No regressions were observed in the control group.


CONCLUSIONS
We cannot exclude the possibility that LF-EM signals transmitted via BRT into the tumor-bearers may stimulate two separate processes: effective immunological response and/or tumor-cell death. The method appears to be capable of inducing the regression of transplantable hepatoma in vivo, thus is a potential subject of further studies.
Fedorowski
Artur
A
Department of Family Medicine, Wroclaw Medical University, Syrokomli Wroclaw, Poland. artur.fedorowski@orebroll.se
Steciwko
Andrzej
A
Rabczynski
Jerzy
J
D000818:Animals; D002455:Cell Division / Q000528:radiation effects; D004195:Disease Models, Animal; D004307:Dose-Response Relationship, Radiation; D004599:Electric Stimulation Therapy / Q000379:methods*; D004574:Electromagnetic Fields; D005260:Female; D008114:Liver Neoplasms, Experimental / Q000473:pathology / Q000532:radiotherapy*; D011829:Radiation Dosage; D011897:Random Allocation; D051381:Rats; D011915:Rats, Inbred BUF; D012074:Remission Induction; D013997:Time Factors