Coat color-tagged green mouse with EGFP expressed from the RNA polymerase II promoter.
Laborious molecular genotyping and variegated gene expression are two widely encountered issues for transgenic mouse studies. To facilitate genotyping in the FVB/N albino background and to reduce variegated expression, we successfully generated double-tagged transgenic mice for direct visual genotyping with the coat color phenotype derived from tyrosinase cDNA driven by the tyrosinase promoter and with simultaneous high enhanced green fluorescent protein (EGFP) expression driven by the promoter of RNA polymerase II large subunit gene. Incorporation of insulator into a transgene construct achieved high efficiency of transgene expression in more than 90% of the founders. EGFP was detected as early as the one-cell fertilized egg and lasted for the whole embryo development, as well as in all of the adult tissues examined. The coat color-tagged green mice offer opportunities in applications such as tissue transplantation, lineage tracing, chimera biology, RNA interference, and other transgenic studies.
Hsiao
Yi-Chun
YC
Faculty of Life Sciences and Institute of Genetics, National Yang-Ming University, Taipei, Taiwan.
Chang
Hsiao-Hui
HH
Tsai
Ching-Yen
CY
Jong
Yiin-Jeng
YJ
Horng
Long-Shyan
LS
Lin
Shu-Fen
SF
Tsai
Ting-Fen
TF
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D002645:Chickens; D018076:DNA, Complementary; D047108:Embryonic Development; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005914:Globins / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006200:Hair Color / Q000235:genetics*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D014442:Monophenol Monooxygenase / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012319:RNA Polymerase II / Q000235:genetics*
D018076:DNA, Complementary; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D005914:Globins; D014442:Monophenol Monooxygenase; D012319:RNA Polymerase II