Immunoassays and sequence-specific DNA detection on a microchip using enzyme amplified electrochemical detection.
A CMOS fabricated silicon microchip was used as a platform for immunoassays and DNA synthesis and hybridization. The chip is covered with a biofriendly matrix wherein the chemistries occur. The active silicon chip has over 1000 active electrodes that can be individually addressed for both synthesis of DNA and protein attachment to a membrane on the chip surface. Additionally, the active chip can be further used for the detection of various analytes at the chip surface via digital read out resulting from the redox enzymes on the captured oligonucleotide or antibody.
Dill
Kilian
K
CombiMatrix Corporation, 6500 Harbour Heights Parkway, Suite 301, Mukilteo, WA 98275, USA. kdill@combimatrix.com
Montgomery
Donald D
DD
Ghindilis
Andrey L
AL
Schwarzkopf
Kevin R
KR
D001435:Bacteriophages / Q000276:immunology / Q000302:isolation & purification; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D004563:Electrochemistry; D004566:Electrodes; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D008027:Light; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D009961:Orosomucoid / Q000032:analysis / Q000276:immunology; D012996:Solutions
D009961:Orosomucoid; D012996:Solutions; D004247:DNA; D006735:Horseradish Peroxidase