title: Isoflavones found in Korean soybean paste as 3-hydroxy-3-methylglutaryl Coenzyme A reductase inhibitors.
abstract: 3-Hydroxy-3-methylglutaryl CoA (HMG-CoA) reductase is the rate-limiting enzyme in the biosynthesis of cholesterol in mammals. Some microbial metabolites have been found to be HMG-CoA reductase inhibitors. Korean soybean paste is a unique food fermented by many microorganisms. The enzymatic method using the catalytic domain of Syrian hamster HMG-CoA reductase was employed for the screening of HMG-CoA reductase inhibitors. Soybean paste extract was fractionated by vacuum liquid chromatography. Fractions showing relatively high HMG-CoA reductase inhibition were further purified through Sephadex LH-20 column chromatography and C18 preparative HPLC, and the inhibitory compounds were identified as genistein, daidzein, and glycitein.
authors.affiliation: Department of Food Science and Technology, School of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, Seoul, Korea.
authors.forename: Jang Hoon
authors.initials: JH
authors.lastname: Sung
authors.forename: Seung Jun
authors.initials: SJ
authors.lastname: Choi
authors.forename: Soon Won
authors.initials: SW
authors.lastname: Lee
authors.forename: Kwan Hwa
authors.initials: KH
authors.lastname: Park
authors.forename: Tae Wha
authors.initials: TW
authors.lastname: Moon
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D019833:Genistein / Q000032:analysis; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D025782:Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent / Q000378:metabolism; D007529:Isoflavones / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013058:Mass Spectrometry; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry*
chemical_list: D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D007529:Isoflavones; D010936:Plant Extracts; C004742:daidzein; C086566:glycitein; D019833:Genistein; D025782:Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent