title: [Immunohistochemical study of in situ CD86 expression in rejected rat corneal graft after penetrating keratoplasty].
abstract: OBJECTIVE
  To investigate the expression of CD86 co-stimulatory molecules in rejected rat corneal graft in situ after penetrating keratoplasty.
  
  
  METHODS
  Rat models of orthotopical corneal transplantation were established, and after the occurrence of graft rejection, immunohistochemical staining was performed on the corneal whole-mounts and the spleen tissue of the recipient rats.
  
  
  RESULTS
  CD86(+) cells were detected in the epithelium of the corneal graft during allograft rejection but not in normal cornea.
  
  
  CONCLUSIONS
  In situ expression of costimulatory molecules in the corneal allograft may be related to the acute immune rejection after penetrating keratoplasty.
authors.affiliation: Department of Ophthalmology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
authors.forename: Lang
authors.initials: L
authors.lastname: Bai
authors.forename: Xiao-he
authors.initials: XH
authors.lastname: Lu
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
authors.forename: Sen-tao
authors.initials: ST
authors.lastname: Dang
authors.forename: Jin-hua
authors.initials: JH
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis*; D018122:B7-1 Antigen / Q000032:analysis; D051940:B7-2 Antigen; D005260:Female; D006084:Graft Rejection / Q000276:immunology*; D007150:Immunohistochemistry; D015948:Keratoplasty, Penetrating / Q000276:immunology*; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D014184:Transplantation, Homologous
chemical_list: D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497966:Cd86 protein, rat; D008562:Membrane Glycoproteins