title: Herpetomonas ztiplika n. sp. (Kinetoplastida: Trypanosomatidae): a parasite of the blood-sucking biting midge Culicoides kibunensis Tokunaga, 1937 (Diptera: Ceratopogonidae).
abstract: Herein, we describe the first case of a natural infection of biting midges by a kinetoplastid protozoan. Flagellates from a female Culicoides kibunensis captured in a bird's nest were introduced into culture and characterized by light and electron microscopy. However, because the morphological data were inconclusive, the novel endosymbiont-free trypanosomatid was assigned into Herpetomonas primarily on the basis of the 18S and 5S ribosomal RNA (rRNA) gene sequences.
authors.affiliation: Institute of Parasitology, Czech Academy of Sciences, Branisovská 31, Ceské Budejovice 37005, Czech Republic.
authors.forename: Sergei
authors.initials: S
authors.lastname: Podlipaev
authors.forename: Jan
authors.initials: J
authors.lastname: Votýpka
authors.forename: Milan
authors.initials: M
authors.lastname: Jirků
authors.forename: Milena
authors.initials: M
authors.lastname: Svobodová
authors.forename: Julius
authors.initials: J
authors.lastname: Lukes
mesh_terms: D000818:Animals; D001483:Base Sequence; D002519:Ceratopogonidae / Q000469:parasitology*; D018105:DNA, Kinetoplast / Q000737:chemistry; D016054:DNA, Protozoan / Q000737:chemistry; D005260:Female; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D014351:Trypanosomatina / Q000145:classification* / Q000235:genetics / Q000648:ultrastructure
chemical_list: D018105:DNA, Kinetoplast; D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S; D012341:RNA, Ribosomal, 5S