title: [Preparation of monoclonal antibodies against morphine and preliminary application of its colloidal gold conjugates].
abstract: OBJECTIVE
  To prepare monoclonal antibodies (mAbs) against morphine.
  
  
  METHODS
  Morphine was conjugated to rabbit serum albumin or bovine serum albumin, respectively and the BALB/c mice were immunized with the conjugated. The mAbs against morphine were obtained by hybridoma technique. The urine samples were detected with the paper chromatography based on crosslinked of the purified mAb with colloidal gold.
  
  
  RESULTS
  Three mAbs against morphine were obtained and the sensitivity detected by paper chromatography reached to 200 microg/L.
  
  
  CONCLUSIONS
  The successful preparation of the mAb and its colloid gold conjugates may be useful for field check.
authors.affiliation: Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Xi
authors.forename: Bo-Quan
authors.initials: BQ
authors.lastname: Jin
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhu
authors.forename: Xue-Song
authors.initials: XS
authors.lastname: Liu
authors.forename: Wei-Xue
authors.initials: WX
authors.lastname: Wang
authors.forename: Xiao-Song
authors.initials: XS
authors.lastname: Li
authors.forename: Han-Yuan
authors.initials: HY
authors.lastname: Wu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D017979:Gold Colloid; D006825:Hybridomas / Q000276:immunology; D008807:Mice, Inbred BALB C; D009020:Morphine
chemical_list: D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D009020:Morphine