title: Crystallization and preliminary X-ray crystallographic analysis of the DNA-binding domain of BldD from Streptomyces coelicolor A3(2).
abstract: The N-terminal DNA-binding domain of BldD from Streptomyces coelicolor A3(2) was crystallized by the hanging-drop vapour-diffusion method at 296 K. A 1.8 angstroms data set has been collected using synchrotron radiation at Pohang Light Source, South Korea. The crystal belongs to the monoclinic space group C2, with unit-cell parameters a = 77.2, b = 31.8, c = 33.6 angstroms, beta = 105.1 degrees. Analysis of the packing density shows that the asymmetric unit probably contains one molecule, with a solvent content of 43.6%.
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, Seoul National University, Seoul 151-742, South Korea.
authors.forename: In Kwon
authors.initials: IK
authors.lastname: Kim
authors.forename: Min Kyu
authors.initials: MK
authors.lastname: Kim
authors.forename: Chang Jin
authors.initials: CJ
authors.lastname: Lee
authors.forename: Hyung Soon
authors.initials: HS
authors.lastname: Yim
authors.forename: Sun Shin
authors.initials: SS
authors.lastname: Cha
authors.forename: Sa Ouk
authors.initials: SO
authors.lastname: Kang
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D001483:Base Sequence; D018360:Crystallography, X-Ray / Q000379:methods*; D004247:DNA / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry*; D004926:Escherichia coli / Q000378:metabolism; D015233:Models, Statistical; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D048372:Streptomyces coelicolor / Q000737:chemistry*; D013696:Temperature; D014157:Transcription Factors / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; C111306:BldD protein, Streptomyces coelicolor; D004268:DNA-Binding Proteins; D011506:Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors; D004247:DNA