[Inducible expression of reconstructed human caspase-6 gene in Hela cells].
OBJECTIVE
To observe the pro-apoptotic effect of RCasp-6 gene in Hela cells.


METHODS
RCasp-6 gene was amplified by PCR and cloned into the pIND vector. Hela cells were transfected with pIND-RCasp-6 and then inducced with ecdyson analogue. Expression of target gene was detected by immunocytochemical staining. Changes of the morphology and growth of Hela cells subjected to transfection of target gene were observed by HE staining and viable cell counting.


RESULTS
RCasp-6 gene was cloned by PCR and its eukaryotic expression vector was successfully reconstructed. Expression of RCasp-6 gene in the transfected Hela cells leads to the morphological changes of the cells. Many of the transfected Hela cells shrunk and some cells were died.


CONCLUSIONS
The expression of reconstructed human caspase-6 gene can efficiently accelerate death of Hela cells.
Xu
Yan-Ming
YM
Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710032,China.
Liu
Rui
R
Hai
Chun-Xu
CX
Wang
Li-Feng
LF
Wang
Ji-Cun
JC
Jin
Ming
M
Wang
Cheng-Ji
CJ
Yang
An-Gang
AG
D017209:Apoptosis; D053178:Caspase 6; D015870:Gene Expression; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D014162:Transfection
D053178:Caspase 6