The fate of mitochondria in Ibex-hirus reconstructed early embryos.
Inter-species nuclear transfer could be used to preserve North Goat (Capra ibex), an endangered species. We established the culture conditions for ibex-hirus reconstructed embryos and optimized the method for DNA extractions of a single cell and early cloned embryo. By using mitochondria-specific probes of ibex and hirus respectively we found that mitochondria of donor cells can co-exist with recipients in 1-cell and 2-cell stages of the reconstructed embryos but not in the following developmental stages.
Jiang
Yan
Y
State Key Laboratory of Reproductive Biology, Institute of Zoology, the Chinese Academy of Sciences, Beijing 100080, China.
Liu
Shu-Zhen
SZ
Zhang
Yan-Ling
YL
Jiang
Man-Xi
MX
Sun
Qing-Yuan
QY
Chen
Da-Yuan
DY
D000818:Animals; D030801:Animals, Genetically Modified / Q000196:embryology; D019976:Cloning, Organism / Q000379:methods*; D004272:DNA, Mitochondrial / Q000235:genetics; D004622:Embryo, Mammalian / Q000502:physiology* / Q000601:surgery*; D005314:Embryonic and Fetal Development / Q000235:genetics; D008928:Mitochondria / Q000502:physiology*; D053652:Nuclear Transfer Techniques; D012418:Ruminants / Q000196:embryology* / Q000235:genetics*
D004272:DNA, Mitochondrial