Effect of the glycosphingolipid, GM1 on localization of dibucaine in phospholipid vesicles: a fluorescence study.
Interaction of the local anesthetic dibucaine with small unilamellar vesicles of dimyristoylphosphatidylcholine (DMPC) and dioleoylphosphatidylcholine (DOPC) containing different mole percents of monosialoganglioside (GM1) has been studied by fluorescence spectroscopy. Fluorescence measurements on dibucaine in the presence of phospholipid vesicles containing various amounts of GM1 yielded a pattern of variation of wavelength at emission maximum and steady-state anisotropy which indicated that the microenvironment of dibucaine is more hydrophobic and rigid in membranes that contain GM1 than in membranes without it. Experiments on quenching of fluorescence from membrane-associated dibucaine by potassium iodide showed reduced quenching efficiency with the increase in GM1 content of the vesicles, demonstrating lesser accessibility of the iodide quenchers to dibucaine in the presence of GM1, when compared to that in its absence. Total emission intensity decay profiles of dibucaine yielded two lifetime components of approximately 1 and approximately 2.8-3.1 ns with mean relative contributions of approximately 25 and approximately 75%, respectively. The mean lifetime in vesicles was 20-30% lower than in the aqueous medium and showed a definite increase in presence of GM1 from that in the absence of it. All the spectral properties point that dibucaine encountered regions of membrane containing significant amount of GM1 and penetrated deeper in hydrophobic core of the bilayer.
Mondal
Mousumi
M
Chemical Sciences Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700064, India. mom2004@med.cornell.edu
Chakrabarti
Abhijit
A
D003992:Dibucaine / Q000737:chemistry* / Q000378:metabolism; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D005453:Fluorescence; D005732:Gangliosides / Q000494:pharmacology*; D006028:Glycosphingolipids / Q000378:metabolism / Q000494:pharmacology*; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism*; D015394:Molecular Structure; D010713:Phosphatidylcholines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D013696:Temperature
D005732:Gangliosides; D006028:Glycosphingolipids; D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; C025447:sialogangliosides; C017251:1,2-oleoylphosphatidylcholine; D003992:Dibucaine; D004134:Dimyristoylphosphatidylcholine