Fenofibrate and fenofibric acid analysis by capillary electrophoresis.
A capillary electrophoresis method has been developed to measure fenofibrate in capsules based on micellar electrokinetic capillary chromatography with detection at 280 nm using a borate buffer containing sodium dodecyl sulfate (SDS). However, the metabolite of this drug (fenofibric acid) in serum and whole blood was analyzed by capillary zone electrophoresis (CZE) in a borate-carbonate buffer using acetonitrile stacking. The analysis is rapid, < 7 min with no interferences. Incubation of fenofibrate in whole blood caused hydrolysis of the ester bond with the release of fenofibric acid.
Shihabi
Zak K
ZK
Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA. zshihabi@wfubmc.edu
D019075:Electrophoresis, Capillary / Q000379:methods*; D011345:Fenofibrate / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood; D006801:Humans; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
D012967:Sodium Dodecyl Sulfate; C006012:fenofibric acid; D011345:Fenofibrate