Erythropoiesis from adult but not fetal blood-derived CD133+ stem cells depends strongly on interleukin-3.
We have shown previously that erythropoiesis in cultures from fetal blood depends less on interleukin-3 (IL3) than erythropoiesis from adult blood. This paper explores if this is due to different proportions of stem cell sub-populations with different IL3 requirement, or to different IL3 requirement on fetal and adult stem cells in general. The CD133 (AC133) antigen is found on half of all CD34+ cells with erythropoietic potential and is thought to mark a primitive stem cell sub-population. This work compares the IL3 requirements of CD133+ and CD133-CD34+ cells derived from fetal (12-20 weeks) and adult blood. Erythropoiesis was monitored using flow cytometry and colony counting. Erythropoiesis from adult CD133+ but not CD133-CD34+ cells was dramatically delayed (6-8 days) in the absence of IL3. In cultures from fetal blood, both CD133+ and CD133-CD34+ cells showed only a small dependency on IL3. The ratio of erythroid colony-forming CD133+ cells to erythroid colony-forming CD34+ cells was 0.5-0.6 in adult and 0.2-0.4 in fetal blood. The differential IL3 dependency of erythropoiesis from fetal and adult blood CD133+ and CD133-CD34+ stem cells may be relevant for the choice and preparation of stem cells for clinical purposes.
Böhmer
Ralph M
RM
Genetic Technologies Pty Ltd., Melbourne, Australia. ralph.bohmer@genetype.com.au
D000071916:AC133 Antigen; D000328:Adult; D015703:Antigens, CD; D018952:Antigens, CD34 / Q000032:analysis; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005260:Female; D005333:Fetus; D005434:Flow Cytometry; D006023:Glycoproteins; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology*; D007700:Kinetics; D008875:Middle Aged; D010455:Peptides; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D015203:Reproducibility of Results
D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D006023:Glycoproteins; D007377:Interleukin-3; C000606023:PROM1 protein, human; D010455:Peptides