title: The in vivo form of the murine class VI POU protein Emb is larger than that encoded by previously described transcripts.
abstract: The class VI POU domain family member known as Emb in the mouse (rat Brn5 or human mPOU/TCFbeta1) is present in vivo as a protein migrating at about 80 kDa on western blots, considerably larger than that predicted (about 42 kDa) from previously cloned coding sequences. By RT-PCR and 5' RACE strategies a full-length Emb sequence, Emb FL, is now identified. Shorter sequences encoding the -COOH terminal, and an -NH(2) terminal isoform, EmbN, were also isolated. Comparisons of Emb coding sequences between species, including the full-length zebra fish, POU(c), are presented, together with a compilation of the multiple transcripts produced by alternative splicing and the presence of different transcriptional start and stop sites, from the Emb gene.
authors.affiliation: C.N.R.S. URA 2578, Department of Developmental Biology, Pasteur Institute, 75724 Paris Cedex 15, France.
authors.forename: F
authors.initials: F
authors.lastname: Relaix
authors.forename: S
authors.initials: S
authors.lastname: Molinari
authors.forename: M
authors.initials: M
authors.lastname: Lemonnier
authors.forename: B
authors.initials: B
authors.lastname: Schäfer
authors.forename: M
authors.initials: M
authors.lastname: Buckingham
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005796:Genes / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D050809:POU Domain Factors; D020033:Protein Isoforms / Q000235:genetics; D051381:Rats; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D050809:POU Domain Factors; C489996:Pou6f1 protein, mouse; C083853:Pou6f1 protein, rat; D020033:Protein Isoforms; D014157:Transcription Factors