The generation of proper constitutive G-tails on yeast telomeres is dependent on the MRX complex.
The precise DNA arrangement at chromosomal ends and the proteins involved in its maintenance are of crucial importance for genome stability. For the yeast Saccharomyces cerevisiae, this constitutive DNA configuration has remained unknown. We demonstrate here that G-tails of 12-14 bases are present outside of S phase on normal yeast telomeres. Furthermore, the Mre11p protein is essential for the proper establishment of this constitutive end-structure. However, the timing of extended G-tails occurring during S phase is not affected in strains lacking Mre11p. Thus, G-tails are present on yeast chromosomes throughout the cell cycle and the MRX complex is required for their normal establishment.
Larrivée
Michel
M
Département de Microbiologie et Infectiologie, Groupe ARN/RNA Group, Faculté de Médecine, Université de Sherbrooke, Sherbrooke, J1H 5N4 Canada.
LeBel
Catherine
C
Wellinger
Raymund J
RJ
D001483:Base Sequence; D002453:Cell Cycle; D015825:Chromosomes, Fungal; D004706:Endodeoxyribonucleases / Q000502:physiology*; D005090:Exodeoxyribonucleases / Q000502:physiology*; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D016196:S Phase; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*; D016615:Telomere / Q000737:chemistry / Q000648:ultrastructure*; D034501:Telomere-Binding Proteins / Q000502:physiology
D029701:Saccharomyces cerevisiae Proteins; D034501:Telomere-Binding Proteins; D004706:Endodeoxyribonucleases; D005090:Exodeoxyribonucleases; C094191:MRE11 protein, S cerevisiae