title: [Expression of RhoC in gastric cancer cell lines and construction and identification of RhoC-specific siRNA expression vector].
abstract: OBJECTIVE
  To study the expression of RhoC in human gastric cancer cell lines and to construct and identify the RhoC-specific siRNA expressing vector.
  
  
  METHODS
  The expression of RhoC in human gastric cancer cell lines was detected by Western blot. According to the computer aided design(CAD),RhoC-specific siRNA gene was synthesized and cloned into the expression vector mU6pro. The constructed RhoC-siRNA was transiently transfected into high-metastatic gastric cancer cell line AGS and the inhibition effect of RhoC-siRNA on expression of RhoC in AGS cells was detected using Western blot.
  
  
  RESULTS
  The expression level of RhoC was up-regulated in high metastatic cells lines. Double enzyme digestion analysis and DNA sequencing confirmed that the RhoC-specific siRNA expression vector was constructed successfully. RhoC expression in AGS cells was specifically suppressed after transfection of RhoC-siRNA.
  
  
  CONCLUSIONS
  The RhoC-specific siRNA expression vector has been successfully constructed, which may provide a novel applicable strategy for gene therapy of gastric cancer.
authors.affiliation: Institute of Digestive Disease, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. liuzhp@edu.cn
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Bi
authors.forename: Yang-lin
authors.initials: YL
authors.lastname: Pan
authors.forename: Yan
authors.initials: Y
authors.lastname: Xue
authors.forename: Zhe-yi
authors.initials: ZY
authors.lastname: Han
authors.forename: Chang-jiang
authors.initials: CJ
authors.lastname: Liu
authors.forename: Dai-ming
authors.initials: DM
authors.lastname: Fan
mesh_terms: D045744:Cell Line, Tumor; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D009362:Neoplasm Metastasis; D034741:RNA, Small Interfering / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D014162:Transfection; D020741:rho GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D000075024:rhoC GTP-Binding Protein
chemical_list: D034741:RNA, Small Interfering; C066690:RHOC protein, human; D020741:rho GTP-Binding Proteins; D000075024:rhoC GTP-Binding Protein