title: Developmental regulation of EVF-1, a novel non-coding RNA transcribed upstream of the mouse Dlx6 gene.
abstract: We previously reported that sonic hedgehog (Shh) induces the differentiation of rat ventral forebrain neurons expressing a novel marker, EVF-1 [Development 125 (1998) 5079]. In this report, we show that EVF-1 is a novel, developmentally regulated, non-coding RNA, with no homology to other known non-coding RNA sequences. Sequence analysis, in vitro translation, and comparison of the rat and mouse EVF-1 sequences suggest that EVF-1 contains no protein coding regions. Chromosomal location indicates that EVF-1 maps adjacent to the Dlx6 gene on mouse chromosome 6. RNA in situ hybridization of the embryonic rat forebrain shows that EVF-1 is expressed by immature neurons in the subventricular zone and its expression decreases during forebrain development. Whole mount in situ hybridization shows that EVF-1 is expressed at high levels in the branchial arches, ventral forebrain, olfactory bulb, and limbs. EVF-1 expression is linked to Shh and the Dlx family of proteins, genes with a demonstrated importance to ventral forebrain and craniofacial development.
authors.affiliation: Program in Neurobiology, Department of Pediatrics, Children's Memorial Institute for Education and Research, Feinberg School of Medicine, Northwestern University, Box No. 209, 2430 North Halsted, Chicago, IL 60614, USA. j-kohtz@northwestern.edu
authors.forename: Jhumku D
authors.initials: JD
authors.lastname: Kohtz
authors.forename: Gord
authors.initials: G
authors.lastname: Fishell
mesh_terms: D000818:Animals; D001934:Branchial Region / Q000196:embryology; D002875:Chromosomes / Q000235:genetics*; D005123:Eye / Q000196:embryology; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology*; D006614:Hindlimb / Q000196:embryology; D018398:Homeodomain Proteins / Q000235:genetics*; D051379:Mice; D009474:Neurons / Q000378:metabolism; D011247:Pregnancy; D016548:Prosencephalon / Q000196:embryology*; D015138:RNA, Nuclear / Q000096:biosynthesis* / Q000235:genetics*; D022661:RNA, Untranslated / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats
chemical_list: C100597:Dlx6 protein, mouse; D018398:Homeodomain Proteins; D015138:RNA, Nuclear; D022661:RNA, Untranslated