title: [The effect of polyporus umbellatus polysaccharide on the immunosuppression property of culture supernatant of S180 cells].
abstract: OBJECTIVE
  To investigate the effect of polyporus umbellatus polysaccharide (PUPS) on the immunosuppression property of tumor cell line S180 culture supernatant.
  
  
  METHODS
  The inhibitory effects of the culture supernatant of S180 cells in the presence or absence of PUPS on the ConA-induced mouse splenocyte proliferation, IL-2 production, killer activity and the reactivity of CTLL-2 cells to IL-2 were detected by MTT colorimetry. The effect of the culture supernatant on the IL-2Ralpha expression on murine splenocytes was detected by flow cytometry.
  
  
  RESULTS
  The culture supernatant of S180 cells could strongly inhibit immunity in terms of the above five indexes, while PUPS could reverse the immunosuppression.
  
  
  CONCLUSIONS
  PUPS can offset the immunosuppression of the supernatant from S180 cell culture, which may be mediated by down-regulating the synthesis and/or secretion of immunosuppressive substance by S180 cells.
authors.affiliation: Department of Immunology, Institute of Basic Medical Science, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Yang
authors.forename: Run-tian
authors.initials: RT
authors.lastname: Wang
authors.forename: Jing-sheng
authors.initials: JS
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Tong
authors.forename: Yu-qing
authors.initials: YQ
authors.lastname: Deng
authors.forename: Quan-hai
authors.initials: QH
authors.lastname: Li
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D005260:Female; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007376:Interleukin-2 / Q000096:biosynthesis; D053645:Interleukin-2 Receptor alpha Subunit; D008297:Male; D051379:Mice; D011124:Polyporaceae / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D012510:Sarcoma 180 / Q000378:metabolism* / Q000473:pathology; D013154:Spleen / Q000166:cytology / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; C508018:Il2ra protein, mouse; D007166:Immunosuppressive Agents; D007376:Interleukin-2; D053645:Interleukin-2 Receptor alpha Subunit; D011134:Polysaccharides; D015375:Receptors, Interleukin-2