Using FM1-43 to study neuropeptide granule dynamics and exocytosis.
In the study of neuropeptide secretion and membrane trafficking, the fluorescent dye FM1-43 provides the ability to label selectively those structures that are undergoing exocytosis and endocytosis in living cells in real time. This review describes the unique properties of the FM dyes that make them ideal for studying neuropeptide granule dynamics and discusses various techniques that take advantage of FM dyes.
Brumback
Audrey C
AC
Department of Biological Sciences, University of Denver, Denver, CO 80208, USA.
Lieber
Janet L
JL
Angleson
Joseph K
JK
Betz
William J
WJ
D000818:Animals; D003594:Cytoplasmic Granules / Q000378:metabolism*; D005089:Exocytosis / Q000502:physiology*; D005456:Fluorescent Dyes; D017930:Genes, Reporter; D005821:Genetic Techniques; D007150:Immunohistochemistry / Q000379:methods; D009479:Neuropeptides / Q000378:metabolism*; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds
C073804:FM1 43; D005456:Fluorescent Dyes; D009479:Neuropeptides; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds