Orthogonal separations of nicotine and nicotine-related alkaloids by various capillary electrophoretic modes.
The migration behaviour of nicotine and related tobacco alkaloids was investigated using three different capillary electrophoretic (CE) modes. Novel separations were achieved both using microemulsion electrokinetic chromatography (MEEKC) and nonaqueous CE (NACE). Improved resolution compared to previous studies was obtained using free-solution CE (FSCE). Each technique resulted in different, orthogonal separation selectivity. The suitability of each method for application to the analysis of nicotine lozenges is discussed. The FSCE method was applied to the analysis of nicotine lozenges due to its compatibility with an established lozenge extraction solvent. The method used gave good injection precision and linearity. Good agreement of CE and high-performance liquid chromatography (HPLC) results was obtained. The CE method is therefore considered suitable for the quantitative determination of nicotine in nicotine lozenges.
Marsh
Alex
A
GlaxoSmithKline R & D, New Frontiers Science Park South, Harlow, Essex, UK. alex.c.marsh@gsk.com
Clark
Brian J
BJ
Altria
Kevin D
KD
D000470:Alkaloids / Q000032:analysis*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D009538:Nicotine / Q000031:analogs & derivatives / Q000032:analysis*
D000470:Alkaloids; D009538:Nicotine