title: Urinary protein fraction in healthy subjects using cellulose acetate membrane electrophoresis followed by colloidal silver staining.
abstract: We previously reported a rapid and highly sensitive colloidal silver staining solution suitable for the cellulose acetate membrane. This method was useful for detecting even very small amounts of urinary protein. In the present study, we examined urinary protein fractions in healthy subjects, using cellulose acetate membrane electrophoresis (CAE) with a highly sensitive colloidal silver staining, in an attempt to determine the clinical relevance of urinary protein fractions. Sixty unconcentrated spot urine specimens were analyzed by CAE and calculated by densitometry. All of the samples were separated into five fractions by CAE. The mean +/- 1 SD of the percentage of five fractions was 28.37 +/- 8.51 in albumin, 4.30 +/- 4.19 in alpha1-globulin, 14.41 +/- 6.14 in alpha2-globulin, 19.45 +/- 7.10 in beta-globulin, and 33.46 +/- 8.24 in gamma-globulin. The albumin/globulin (A/G) ratio was 0.41 +/- 0.17. These six items and the concentrations of total protein, albumin, and beta-N-acetyl-D-glucosaminidase (NAG) did not significantly differ between males and females. NAG is the marker of tubulointerstitial nephropathy. The results suggest that there are no gender-dependent differences in the urinary protein fractions of healthy subjects.
authors.affiliation: Analytical Laboratory Chemistry, Graduate School of Allied Health Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Ryoko
authors.initials: R
authors.lastname: Machii
authors.forename: Ryo
authors.initials: R
authors.lastname: Kubota
authors.forename: Nobuo
authors.initials: N
authors.lastname: Hiratsuka
authors.forename: Kayo
authors.initials: K
authors.lastname: Sugimoto
authors.forename: Rie
authors.initials: R
authors.lastname: Masudo
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Kurihara
authors.forename: Shizuko
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Shiba
mesh_terms: D000328:Adult; D003102:Colloids; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D011506:Proteins / Q000032:analysis*; D016622:Silver Staining / Q000379:methods*; D014556:Urine / Q000737:chemistry*
chemical_list: D003102:Colloids; D011506:Proteins