The Drosophila fragile X-related gene regulates axoneme differentiation during spermatogenesis.
Macroorchidism (i.e., enlarged testicles) and mental retardation are the two hallmark symptoms of Fragile X syndrome (FraX). The disease is caused by loss of fragile X mental retardation protein (FMRP), an RNA-binding translational regulator. We previously established a FraX model in Drosophila, showing that the fly FMRP homologue, dFXR, acts as a negative translational regulator of microtubule-associated Futsch to control stability of the microtubule cytoskeleton during nervous system development. Here, we investigate dFXR function in the testes. Male dfxr null mutants have the enlarged testes characteristic of the disease and are nearly sterile (>90% reduced male fecundity). dFXR protein is highly enriched in Drosophila testes, particularly in spermatogenic cells during the early stages of spermatogenesis. Cytological analyses reveal that spermatogenesis is arrested specifically in late-stage spermatid differentiation following individualization. Ultrastructurally, dfxr mutants lose specifically the central pair microtubules in the sperm tail axoneme. The frequency of central pair microtubule loss becomes progressively greater as spermatogenesis progresses, suggesting that dFXR regulates microtubule stability. Proteomic analyses reveal that chaperones Hsp60B-, Hsp68-, Hsp90-related protein TRAP1, and other proteins have altered expression in dfxr mutant testes. Taken together with our previous nervous system results, these data suggest a common model in which dFXR regulates microtubule stability in both synaptogenesis in the nervous system and spermatogenesis in the testes. The characterization of dfxr function in the testes paves the way to genetic screens for modifiers of dfxr-induced male sterility, as a means to efficiently dissect FMRP-mediated mechanisms.
Zhang
Yong Q
YQ
Department of Biological Sciences, Vanderbilt Kennedy Center for Research on Human Development, Vanderbilt University, Nashville, TN 37235-1634, USA.
Matthies
Heinrich J G
HJ
Mancuso
Joel
J
Andrews
Hillary K
HK
Woodruff
Elvin
E
Friedman
David
D
Broadie
Kendal
K
D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D004330:Drosophila / Q000235:genetics* / Q000502:physiology; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D051860:Fragile X Messenger Ribonucleoprotein 1; D005600:Fragile X Syndrome / Q000235:genetics*; D007150:Immunohistochemistry; D008297:Male; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D008870:Microtubules / Q000000981:diagnostic imaging / Q000502:physiology*; D018832:Molecular Chaperones / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D013082:Sperm Tail / Q000000981:diagnostic imaging; D013091:Spermatogenesis / Q000235:genetics / Q000502:physiology*; D013737:Testis / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D014463:Ultrasonography
D029721:Drosophila Proteins; D051860:Fragile X Messenger Ribonucleoprotein 1; D018832:Molecular Chaperones; D016601:RNA-Binding Proteins; C467328:FMR1 protein, Drosophila