Transcription level of granule-associated phaP and phaR genes and granular morphogenesis of poly-beta-hydroxyalkanoate granules in Ralstonia eutropha.
The transcription levels of the granule-associated phaP and phaR genes in Ralstonia eutropha were regulated through the transformation of the phbC genes from R. eutropha and Alcaligenes latus into the poly-beta-hydroxybutyrate synthase-negative mutant. The granular morphogenesis of short chain length, poly-beta-hydroxyalkanoate (scl-PHA) was closely associated with the mRNA transcription levels of the phaP and phaR genes, especially with the ratio of phaP/phaR genes. The phasin protein encoded by the phaP gene increased the number of granules, while the PhaR protein of the phaR gene enlarged the size of the scl-PHA granules in R. eutropha.
Seo
Min-Chul
MC
Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University, Daegu 702-701, S. Korea.
Shin
Hyun-Dong
HD
Lee
Yong-Hyun
YH
D000217:Acyltransferases / Q000096:biosynthesis / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D020118:Cupriavidus necator / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure*; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C118828:PHAP protein, Bacteria; D012333:RNA, Messenger; D012097:Repressor Proteins; D000217:Acyltransferases; C060970:poly-beta-hydroxybutyrate polymerase