title: [The expression of truncated YggG protein and preparation of its polyclonal antibody].
abstract: OBJECTIVE
  To express truncated YggG protein (TYP) in Escherichia coli and to prepare anti-TYPE antibody.
  
  
  METHODS
  Truncated yggg gene was amplified by PCR from the plasmid containing full length yggg gene and was cloned into the expression vector pDH2. The expression of TYP was achieved by thermal induction. Antiserum was prepared by immunizing rabbit with TYP,The titer and specificity of polyclonal antibody were detected by Western blot.
  
  
  RESULTS
  Thin-layer scan analysis showed that TYP accounted for about 37.1% of total bacterial protein. The antiserum was about 1:2,000 in titier and highly specific. Full-length rggG protein could also be recognized by the antiserum.
  
  
  CONCLUSIONS
  The TYP was highly expressed, and specific anti-TYP antiserum was prepared successfully.
authors.affiliation: Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.forename: Su-min
authors.initials: SM
authors.lastname: Chen
authors.forename: Nan-chun
authors.initials: NC
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Xiao-nan
authors.initials: XN
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D005798:Genes, Bacterial; D005822:Genetic Vectors; D007106:Immune Sera / Q000096:biosynthesis* / Q000276:immunology; D010957:Plasmids; D011817:Rabbits; D014169:Transformation, Bacterial
chemical_list: D029968:Escherichia coli Proteins; D007106:Immune Sera