title: [Effects of total alkaloids of Tongbiling prescription on Th1 type cytokine expression in T lymphocytes].
abstract: OBJECTIVE
  To study the effects of total alkaloid of Tongbiling prescription(TBL) on Th1 type cytokine expression in T cells in order to elucidate the anti-inflammatory mechanism of TBL.
  
  
  METHODS
  The lymphocytes were isolated from mouse mesenteric lymph nodes and cultured in-vitro. Various concentrations of TBL were added to the culture followed by phorbol ester and inomycin treatment and then incubated for another 4 hours. The expressions of IFN-gamma and TNF-alpha in the lymphocytes were analyzed by flow cytometry.
  
  
  RESULTS
  200 mg/L and 100 mg/L TBL could obviously inhibit IFN-gamma and TNF-alpha expressions in T lymphocytes.
  
  
  CONCLUSIONS
  Inhibiting Th1 cytokine expression may be one mechanism by which TBL can treat rheumatoid arthritis.
authors.affiliation: Laboratory of Immunology, Guangzhou University of TCM, Guangzhou 510405, China. leexiaojuan@263.net
authors.forename: Xiao-juan
authors.initials: XJ
authors.lastname: Li
authors.forename: Guang-xing
authors.initials: GX
authors.lastname: Chen
authors.forename: Liang
authors.initials: L
authors.lastname: Liu
authors.forename: Pei-xun
authors.initials: PX
authors.lastname: Wang
authors.forename: Ying-jie
authors.initials: YJ
authors.lastname: Hu
authors.forename: Yiao-ying
authors.initials: YY
authors.lastname: Zeng
authors.forename: Ji-fan
authors.initials: JF
authors.lastname: Chen
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D001172:Arthritis, Rheumatoid / Q000188:drug therapy / Q000276:immunology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D007371:Interferon-gamma / Q000032:analysis*; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
chemical_list: D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma