Lotus hairy roots expressing inducible arginine decarboxylase activity.
Biotechnological uses of plant cell-tissue culture usually rely on constitutive transgene expression. However, such expression of transgenes may not always be desirable. In those cases, the use of an inducible promoter could be an alternative approach. To test this hypothesis, we developed two binary vectors harboring a stress-inducible promoter from Arabidopsis thaliana, driving the beta-glucuronidase reporter gene and the oat arginine decarboxylase. Transgenic hairy roots of Lotus corniculatus were obtained with osmotic- and cold-inducible beta-glucuronidase and arginine decarboxylase activities. The increase in the activity of the latter was accompanied by a significant rise in total free polyamines level. Through an organogenesis process, we obtained L. corniculatus transgenic plants avoiding deleterious phenotypes frequently associated with the constitutive over-expression of arginine decarboxylation and putrescine accumulation.
Chiesa
María A
MA
Unidad de Biotecnología 1, IIB-INTECH/UNSAM-CONICET, Ruta Circunvalación Laguna, km. 6 cc 164, (7130) Chascomús, Provincia de Buenos Aires, Argentina.
Ruiz
Oscar A
OA
Sánchez
Diego H
DH
D017360:Arabidopsis / Q000235:genetics; D018554:Avena / Q000201:enzymology; D002262:Carboxy-Lyases / Q000096:biosynthesis / Q000378:metabolism; D046508:Culture Techniques; D005822:Genetic Vectors; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D000070116:Lotus / Q000235:genetics*; D009995:Osmosis; D010641:Phenotype; D018517:Plant Roots / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D011073:Polyamines / Q000737:chemistry / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011700:Putrescine / Q000378:metabolism; D013997:Time Factors; D019076:Transgenes
D011073:Polyamines; D005966:Glucuronidase; D002262:Carboxy-Lyases; C019722:arginine decarboxylase; D011700:Putrescine