title: Selenium distribution in a Se-enriched mushroom species of the genus Ganoderma.
abstract: Data reported here show that Ganoderma lucidum could biotransform inorganic selenite in the substrate into organic forms by intergrating Se into proteins (56-61%) and polysaccharides (11-18%) and other components. Furthermore, water- and alkaline-soluble protein components were mainly responsible for the storage of organic Se, and Se-Met accounts for only a minor (8.2-18.3%) amount of the selenocompounds present in proteins. The molecular mass of most proteins or protein subunits containing Se was no more than 16 kDa. A low concentration of Se (<100 microg/g) in the substrate facilitated the synthesis of total protein and amino acids in G. lucidum, but a high concentration of Se (>150 microg/g) played a reverse role. Additionally, Se concentration in the culture had no significant effect on the distribution of the amino acids and proteins.
authors.affiliation: DAPR Laboratory, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.forename: Guanghua
authors.initials: G
authors.lastname: Zhao
authors.forename: Zhengdong
authors.initials: Z
authors.lastname: Zhao
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.forename: Jiyun
authors.initials: J
authors.lastname: Tong
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Hu
mesh_terms: D000596:Amino Acids / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis / Q000737:chemistry; D032902:Ganoderma / Q000737:chemistry*; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry; D012643:Selenium / Q000032:analysis*; D051140:Selenoproteins; D014018:Tissue Distribution
chemical_list: D000596:Amino Acids; D005656:Fungal Proteins; D011506:Proteins; D051140:Selenoproteins; D012643:Selenium