Teratogenesis in Fundulus heteroclitus embryos exposed to a creosote-contaminated sediment extract and CYP1A inhibitors.
The goal of these experiments was to explore the relationship between cytochrome P4501A (CYP1A) induction and the teratogenicity of sediments from the Atlantic Wood Industries Superfund site (Elizabeth River, VA) in Fundulus heteroclitus embryos. In these experiments we used embryos spawned from reference site adults to assess CYP1A activity and teratogenicity induced by aqueous Elizabeth River sediment extracts (ERSE). Embryo exposures to ERSE induced CYP1A activity and caused deformities, including pericardial edema, heart elongation and tail shortening. Co-exposures with various CYP1A inhibitors significantly decreased CYP1A activity and increased the teratogenicity of the sediment extract. Potential mechanisms for this increased toxicity are discussed herein.
Wassenberg
Deena M
DM
Nicholas School of the Environment and Earth Sciences and Integrated Toxicology Program, Duke University, P.O. Box 90328, Durham, NC 27708, USA. deena@duke.edu
Di Giulio
Richard T
RT
D000014:Abnormalities, Drug-Induced; D000818:Animals; D001571:Benzoflavones / Q000494:pharmacology; D003407:Creosote / Q000633:toxicity*; D019363:Cytochrome P-450 CYP1A1 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000187:drug effects; D004785:Environmental Pollutants / Q000633:toxicity*; D005449:Fluorenes / Q000494:pharmacology; D005618:Fresh Water; D023781:Fundulidae; D019015:Geologic Sediments / Q000032:analysis*; D006330:Heart Defects, Congenital; D010063:Ovum / Q000378:metabolism; D010882:Piperonyl Butoxide / Q000494:pharmacology; D013623:Tail / Q000002:abnormalities; D014768:Virginia
D001571:Benzoflavones; D004785:Environmental Pollutants; D005449:Fluorenes; C007738:fluoranthene; C011512:alpha-naphthoflavone; D003407:Creosote; D019363:Cytochrome P-450 CYP1A1; D010882:Piperonyl Butoxide