Analysis of strychnine from detoxified Strychnos nux-vomica [corrected] seeds using liquid chromatography-electrospray mass spectrometry.
The content of strychnine from Strychnos nux-vomica [corrected] seeds was analyzed and compared to processed seeds by the HPLC-ESI/MS method. Using this technique, levels as low as 1 ng of strychnine were detected. In contrast to conventional UV detectors, this method also made it possible to discriminate brucine. This study resulted in finding the content of strychnine in detoxified seeds to be one tenth of unprocessed Strychnos nux-vomica [corrected] seeds.
Choi
Young Hae
YH
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
Sohn
You-Min
YM
Kim
Chul Young
CY
Oh
Kwan Yul
KY
Kim
Jinwoong
J
D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D012639:Seeds; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013331:Strychnine / Q000737:chemistry / Q000302:isolation & purification*; D031461:Strychnos nux-vomica
D010936:Plant Extracts; D013331:Strychnine