Direct analysis of selected N-acyl-L-homoserine lactones by gas chromatography/mass spectrometry.
A rapid, simple and selective method involving direct separation by gas chromatography (GC) with electron ionization mass spectrometry (EI-MS) was employed to determine some N-acylhomoserine lactones (AHLs). Using GC/EI-MS, simultaneous separation and characterization of AHLs were possible without prior derivatization. Informative fragmentation patterns were obtained to identify the structures of N-acyl chains of AHLs. Electron ionization resulted in a common fragmentation pattern with the most abundant ion at m/z 143 and other minor peaks at m/z 71, 57, and 43. The presence of AHLs in extracts of Burkholderia cepacia strains was achieved in selected ion monitoring mode by using the prominent fragment at m/z 143.
Cataldi
T R I
TR
Dipartimento di Chimica, Università degli Studi della Basilicata, Via N. Sauro 85, 85100 Potenza, Italy. cataldi@unibas.it
Bianco
G
G
Frommberger
M
M
Schmitt-Kopplin
Ph
P
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry; D000215:Acylation; D016956:Burkholderia cepacia / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006714:Homoserine / Q000032:analysis* / Q000737:chemistry
C104908:N-hexanoyl-L-homoserine lactone; C088386:homoserine lactone; D006714:Homoserine; D015107:4-Butyrolactone