PBAN stimulation of pheromone biosynthesis by inducing calcium influx in pheromone glands of Helicoverpa zea.
Isolated pheromone glands of Helicoverpa zea were utilized to investigate the physiological action of pheromone biosynthesis activating neuropeptide (PBAN) with regard to the role of calcium ions in stimulating pheromone biosynthesis under various incubation conditions. Incubation of glands with 1 microM or 1 nM PBAN produced a significant amount of pheromone after a 5 min incubation period and reached maximum pheromone production after 30 min. Glands incubated with PBAN for 1 min, and then without PBAN for 30 min, produced pheromone whether or not extracellular calcium was present during the first 1 min. The presence of lanthanum as a calcium channel blocker did not affect pheromone production if present during the first 1 min of incubation with PBAN. However, if calcium was absent or lanthanum ion was present during the 30 min of incubation, no pheromone was produced. A maximum amount of pheromone was reached when glands were incubated for 1 min with PBAN and for 10 min without PBAN, and repeated three times. The present results indicate that a time interval exists between PBAN binding to a receptor and opening of extracellular calcium channels. Calcium influx into the cytosol from extracellular stores is required for PBAN to stimulate pheromone production. This could be achieved by PBAN either binding periodically to the receptor or the plasma membrane calcium channel could remain activated for a period of time after the initial activation.
Choi
Man-Yeon
MY
Department of Entomology, Iowa State University, 407 Science II, Ames 50011-3222, USA.
Jurenka
Russell A
RA
D000818:Animals; D002118:Calcium / Q000502:physiology*; D015220:Calcium Channels / Q000378:metabolism; D005088:Exocrine Glands / Q000378:metabolism*; D009036:Moths / Q000502:physiology*; D009479:Neuropeptides / Q000502:physiology*; D009924:Organ Culture Techniques; D011956:Receptors, Cell Surface / Q000378:metabolism; D015290:Second Messenger Systems / Q000502:physiology; D012724:Sex Attractants / Q000096:biosynthesis*; D015398:Signal Transduction / Q000502:physiology
D015220:Calcium Channels; D009479:Neuropeptides; D011956:Receptors, Cell Surface; D012724:Sex Attractants; C079709:pheromone biosynthesis activating neuropeptide, Helicoverpa zea; D002118:Calcium