Embryonic chromosomal abnormalities obtained after rescue intracytoplasmic sperm injection of 1-day-old unfertilized oocytes.
OBJECTIVE
To study if second day intracytoplasmic sperm injection (ICSI) results in chromosomal abnormalities in the embryos.


METHODS
Rescue ICSI was performed on 14 metaphase II (MII) oocytes after unsuccessful conventional IVF, four were fertilized. Fluorescent in situ hybridization (FISH) was performed on these four embryos and was informative for three.


RESULTS
There were two tetraploid embryos, one mosaic embryo with trisomy 21, tetrasomy 18, and tetrasomy for sex chromosomes in one cell and trisomy 22 in another cell.


CONCLUSIONS
We discourage the use of second day ICSI due to the observed increase in chromosomal abnormalities in these embryos.
Pehlivan
Tugce
T
Instituto Valenciano de Infertilidad Valencia, Spain.
Rubio
Carmen
C
Ruiz
Amparo
A
Navarro
Jose
J
Remohí
José
J
Pellicer
Antonio
A
Simón
Carlos
C
D000328:Adult; D002869:Chromosome Aberrations; D004622:Embryo, Mammalian / Q000002:abnormalities*; D047108:Embryonic Development; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D009865:Oocytes; D011247:Pregnancy; D013048:Specimen Handling; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013094:Spermatozoa / Q000473:pathology; D013997:Time Factors; D017211:Treatment Failure