title: Membrane-associated prostaglandin E synthase-1 is required for neuropathic pain.
abstract: It is widely accepted that prostaglandin (PG) E2 is the principal pro-inflammatory prostanoid and plays an important role in inflammatory pain. However whether PGE2 is involved in neuropathic pain remains unknown. PGE2 is produced from arachidonic acid via PGH2 by at least three PGE synthases (PGES), cytosolic PGES (cPGES), and membrane-associated PGES (mPGES)-1 and -2. In the present study, to clarify the involvement of PGE2 and identify PGES mediating neuropathic pain, we applied a neuropathic pain model prepared by L5 spinal nerve transection to mPGES-1 knockout (mPGES-1-/-) mice. Whereas they retained normal nociceptive responses, mPGES-1-/- mice did not exhibit mechanical allodynia and thermal hyperalgesia over a week. These results demonstrate that PGE2 produced by mPGES-1 is involved in neuropathic pain.
authors.affiliation: Department of Medical Chemistry, Kansai Medical University, 10-15 Fumizono, Moriguchi 570-8506, Japan.
authors.forename: Tamaki
authors.initials: T
authors.lastname: Mabuchi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kojima
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Abe
authors.forename: Kunio
authors.initials: K
authors.lastname: Takagi
authors.forename: Madoka
authors.initials: M
authors.lastname: Sakurai
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ohmiya
authors.forename: Satoshi
authors.initials: S
authors.lastname: Uematsu
authors.forename: Shizuo
authors.initials: S
authors.lastname: Akira
authors.forename: Kikuko
authors.initials: K
authors.lastname: Watanabe
authors.forename: Seiji
authors.initials: S
authors.lastname: Ito
mesh_terms: D000818:Animals; D003714:Denervation; D015232:Dinoprostone / Q000378:metabolism; D019746:Intramolecular Oxidoreductases / Q000235:genetics* / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009437:Neuralgia / Q000378:metabolism* / Q000503:physiopathology*; D000071876:Prostaglandin-E Synthases; D012333:RNA, Messenger / Q000032:analysis; D013127:Spinal Nerves / Q000502:physiology
chemical_list: D008565:Membrane Proteins; D012333:RNA, Messenger; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; C000605865:Ptges protein, mouse; D015232:Dinoprostone