[Genotoxicity of a new NanoHA-PA66 root filling material in vitro].
OBJECTIVE
The micronucleus test was applied to evaluate the genotoxicity of a new nanocomplex HA-PA66 root filling material in vitro.


METHODS
The dulbecco's modified eagle media(DMEM) extracts of the powder part and the mixture of the new nanomaterial were prepared separately. The V79 cell was used as the test cell and the mitomycin C(MMC) as the positive control. The MTT assay was employed in our study to evaluate the cytotoxic effect while the number of micronucleus was used as the criteria for the detection of genotoxocity.


RESULTS
The MTT values in test groups and negative group were not significantly different at different times (P > 0.05). The number of micronucleus in test groups (powder group: 6.1 +/- 1.1/1,000; complex group: 5.7 +/- 0.6/1,000) was similar to the negative control(5.3 +/- 0.8/1,000, P > 0.05), while they were significantly different to the positive control(123.9 +/- 8/1,000, P < 0.05).


CONCLUSIONS
The new nanocomplex HA-PA66 root filling material showed no detectable cytotoxic and genotoxic effects in this study and was proved to be biocompatible.
Ye
Ling
L
Dept. of Endodontics, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
Su
Qin
Q
Zhou
Xue-dong
XD
Tan
Hong
H
D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity*; D006224:Cricetinae; D003412:Cricetulus; D017886:Durapatite / Q000633:toxicity; D015162:Micronucleus Tests / Q000379:methods; D009152:Mutagenicity Tests / Q000379:methods; D036103:Nanotechnology; D009757:Nylons / Q000633:toxicity; D012387:Root Canal Filling Materials / Q000633:toxicity*
D001672:Biocompatible Materials; D009757:Nylons; D012387:Root Canal Filling Materials; D017886:Durapatite