[Construction and characterization of osteosarcoma 9901 cell cDNA library].
OBJECTIVE
To construct human osteosarcoma 9901 cell cDNA expression library for screening osteosarcoma-specific antigens.


METHODS
Total RNA was extracted from human osteosarcoma cell line 9901 and mRNA was purified. cDNA was synthesized by reverse transcription, and cDNA fragments larger than 400 bp were ligated with dephosphorylated arms of lambdagt11. The recombinants were packaged in-vitro, and a small portion of packaged phage was used to infect E.coli Y1090 for titration. The size of cDNA inserts and the diversity of library were detected by PCR.


RESULTS
The osteosarcoma 9901 cell line cDNA library consisting of 1.5x10(6) recombinant bacteriophages was constructed. The average length of exogenous inserts in the recombinants was about 1.4 kb.


CONCLUSIONS
The cDNA library reported herein is suitable for screening osteosarcoma-specific antigens.
Liao
Bo
B
Department of Orthopedics, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. liaobo_@163.com
Ma
Bao-an
BA
Zhang
Hui-zhong
HZ
Long
Hua
H
Fan
De-gang
DG
Fan
Qing-yu
QY
D001859:Bone Neoplasms / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D012516:Osteosarcoma / Q000235:genetics*; D016133:Polymerase Chain Reaction