title: Genomic polymorphism of human papillomavirus type 52 predisposes toward persistent infection in sexually active women.
abstract: We investigated the role of human papillomavirus (HPV) type 52 polymorphism in the persistence of HPV infection, which is a predictor for cervical lesions. Cervical samples obtained at 6-month intervals were tested for HPV-52 in 1055 women; 41, 12, and 58 women had persistent, transient, and unclassified HPV-52 infections, respectively. HPV-52 isolates were analyzed by polymerase chain-reaction sequencing of the long control region (LCR), E6, and E7 genes. Although age (odds ratio [OR], 0.90 [95% confidence interval [CI], 0.81-0.99]), nonprototypic LCR (OR, 9.26 [95% CI, 2.1-41.7]), and E6 variant (OR, 7.04 [95% CI, 1.4-37]) were associated, in univariate analysis, with the persistence of HPV-52 infection, a nonprototypic LCR variant was the only independent predictor of it (OR, 14.1 [95% CI, 1.1-200]). In the latter variants, the loss of a binding site for a repressor of HPV expression was associated with the persistence of HPV infection (OR, 7.25 [95% CI, 1.67-31.25]).
authors.affiliation: Laboratoire de Virologie Moléculaire, Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Québec, Canada.
authors.forename: Joséphine
authors.initials: J
authors.lastname: Aho
authors.forename: Catherine
authors.initials: C
authors.lastname: Hankins
authors.forename: Cécile
authors.initials: C
authors.lastname: Tremblay
authors.forename: Pierre
authors.initials: P
authors.lastname: Forest
authors.forename: Karina
authors.initials: K
authors.lastname: Pourreaux
authors.forename: Fabrice
authors.initials: F
authors.lastname: Rouah
authors.forename: François
authors.initials: F
authors.lastname: Coutlée
mesh_terms: D000328:Adult; D002584:Cervix Uteri / Q000821:virology; D015331:Cohort Studies; D003430:Cross-Sectional Studies; D004279:DNA, Viral; D005260:Female; D016679:Genome, Viral; D018023:HIV Seronegativity; D006679:HIV Seropositivity / Q000150:complications; D006801:Humans; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000503:physiopathology / Q000821:virology; D011110:Polymorphism, Genetic; D015995:Prevalence; D012725:Sexual Behavior; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000453:epidemiology / Q000821:virology
chemical_list: D004279:DNA, Viral