A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology.
The Mediator is a multiprotein transcriptional coactivator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factors. In the work described here, we exploit multidimensional protein identification technology (MudPIT) to carry out a proteomic analysis of the subunit composition of the mammalian Mediator complex. By comparing MudPIT data sets obtained from six independent Mediator preparations immunoaffinity purified through their Nut2 (MED10), Med25 (MED9), Intersex (MED29), LCMR1 (MED19), AK007855 (MED28), or CRSP70 (MED26) subunits, we identify a set of consensus mammalian Mediator subunits. In addition, we identify as Mediator-associated proteins the CDK8-like cyclin-dependent kinase CDK11 and the TRAP240-like KIAA1025 protein (MED13L), which is mutated in patients with the congenital heart defect transposition of the great arteries (TGA).
Sato
Shigeo
S
Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA.
Tomomori-Sato
Chieri
C
Parmely
Tari J
TJ
Florens
Laurence
L
Zybailov
Boris
B
Swanson
Selene K
SK
Banks
Charles A S
CA
Jin
Jingji
J
Cai
Yong
Y
Washburn
Michael P
MP
Conaway
Joan Weliky
JW
Conaway
Ronald C
RC
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D046911:Macromolecular Substances; D056892:Mediator Complex; D046912:Multiprotein Complexes; D009687:Nuclear Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D021122:Protein Subunits / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D040901:Proteomics / Q000379:methods*; D012319:RNA Polymerase II / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D015534:Trans-Activators / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D014157:Transcription Factors / Q000032:analysis / Q000737:chemistry / Q000235:genetics
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D029721:Drosophila Proteins; C468125:IX protein, Drosophila; C480067:MED13L protein, human; C483045:MED25 protein, human; C491485:MED6 protein, human; D046911:Macromolecular Substances; C491486:Med6 protein, Drosophila; D056892:Mediator Complex; D046912:Multiprotein Complexes; C113770:NUT2 protein, S cerevisiae; D009687:Nuclear Proteins; D021122:Protein Subunits; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C447612:CDK11a protein, human; D018844:Cyclin-Dependent Kinases; D012319:RNA Polymerase II