DNA methylation of multiple genes and clinicopathological relationship of non-small cell lung cancers.
Aberrant methylation of 5' CpG islands is thought to play an important role in the inactivation of tumor suppressor genes in several types of cancers. In non-small cell lung cancer (NSCLC), several genes are known to be frequently methylated and the correlation of their methylation with clinical features has been studied. We determined the methylation of p16, CDH13 and RAR-beta which were reported to be methylated frequently in NSCLCs and HPP-1 which was known to be methylated in other types of cancers. The correlation between methylation and clinicopathological features were examined. The frequencies of methylation in NSCLCs were 20% for p16, 37% for CDH13, 34% for RAR-beta, and 13% for HPP1. The methylation of p16 is correlated with smoking history and methylation of HPP1 was significantly more frequent in adenocarcinomas than in squamous cell carcinomas. This is the first description of aberrant methylation of the HPP1 gene in lung cancers and our data support the previous reports on methylation in NSCLCs and association with smoking.
Hanabata
Tetsuro
T
Department of Cancer and Thoracic Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700-8558, Japan.
Tsukuda
Kazunori
K
Toyooka
Shinichi
S
Yano
Masaaki
M
Aoe
Motoi
M
Nagahiro
Itaru
I
Sano
Yoshifumi
Y
Date
Hiroshi
H
Shimizu
Nobuyoshi
N
D000230:Adenocarcinoma / Q000145:classification / Q000235:genetics; D000368:Aged; D001483:Base Sequence; D014408:Biomarkers, Tumor; D015820:Cadherins / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000145:classification* / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D002294:Carcinoma, Squamous Cell / Q000145:classification / Q000235:genetics; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics; D019175:DNA Methylation; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000235:genetics*; D015226:Dinucleoside Phosphates / Q000235:genetics; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D008175:Lung Neoplasms / Q000145:classification* / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics; D018168:Receptors, Retinoic Acid / Q000235:genetics
D014408:Biomarkers, Tumor; D015820:Cadherins; D019941:Cyclin-Dependent Kinase Inhibitor p16; D017931:DNA Primers; D004273:DNA, Neoplasm; D015226:Dinucleoside Phosphates; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D018168:Receptors, Retinoic Acid; C099972:H-cadherin; C423567:TMEFF2 protein, human; C082925:retinoic acid receptor beta; C015772:CpG dinucleotide