title: Simultaneous PCR detection of ica cluster and methicillin and mupirocin resistance genes in catheter-isolated Staphylococcus.
abstract: Recent data show that more than 50% of catheter-associated bloodstream infections are caused by staphylococci. Staphylococcal infections produced by intercellular-adhesion cluster (ica) carriers can be even more problematic due to the presence of methicillin and mupirocin resistance genes. In the present study, a multiplex PCR protocol that allows the simultaneous identification of staphylococci and detection of both the ica and methicillin and/or mupirocin resistance genes was developed. Furthermore, the method allows differential detection of the ica locus from Staphylococcus aureus and Staphylococcus epidermidis.
authors.affiliation: Molecular Biology Laboratory, Research Unit, Nuestra Señora de Candelaria University Hospital, Santa Cruz de Tenerife, 38010 Santa Cruz de Tenerife, Spain.
authors.forename: Juana V
authors.initials: JV
authors.lastname: Martín-López
authors.forename: Oscar
authors.initials: O
authors.lastname: Díez-Gil
authors.forename: Manuel
authors.initials: M
authors.lastname: Morales
authors.forename: Ninivé
authors.initials: N
authors.lastname: Batista
authors.forename: Jesús
authors.initials: J
authors.lastname: Villar
authors.forename: Félix
authors.initials: F
authors.lastname: Claverie-Martín
authors.forename: Sebastián
authors.initials: S
authors.lastname: Méndez-Alvarez
mesh_terms: D001483:Base Sequence; D002408:Catheters, Indwelling; D017931:DNA Primers; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics*; D016712:Mupirocin / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods; D013210:Staphylococcus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification
chemical_list: D017931:DNA Primers; D016712:Mupirocin