Recovery of glucocorticoid-related loss of synaptic density in the fetal sheep brain at 0.75 of gestation.
Antenatal glucocorticoids routinely used to accelerate fetal lung maturation in human pregnancy at risk of preterm delivery decrease synaptic density and complex electrocortical activity in the fetal sheep brain at 0.87 gestation. We examined whether the effects of betamethasone on synaptic density depend on maturation of hypothalamo-pituitary-adrenal (HPA) axis and whether these effects are reversible. Betamethasone infusion to fetal sheep comparable to the dose used clinically (3.3 microg kg(-1) h(-1) over 48 h) at 0.75 gestation and, thus, before the prepartum increase of cortisol, reduced synaptophysin immunoreactivity (SY-IR) in the frontal neocortex, caudate putamen and hippocampus (P < 0.05). Loss of SY-IR exceeded that shown previously at 0.87 gestation (P < 0.05). It was not accompanied by neuronal damage and was reversible within 24h. In conclusion, fetal betamethasone exposure induces a gestational age-dependent decrease of synaptic density that is transient and more severe in younger fetuses.
Colberg
Claudia
C
Department of Neurology, Friedrich Schiller University, D-07740 Jena, Germany.
Antonow-Schlorke
Iwa
I
Müller
Thomas
T
Schubert
Harald
H
Witte
Otto W
OW
Schwab
Matthias
M
D000818:Animals; D001623:Betamethasone / Q000494:pharmacology; D001921:Brain / Q000187:drug effects* / Q000196:embryology* / Q000648:ultrastructure; D001923:Brain Chemistry / Q000187:drug effects; D005260:Female; D005865:Gestational Age; D005938:Glucocorticoids / Q000633:toxicity*; D006854:Hydrocortisone / Q000378:metabolism; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects; D007150:Immunohistochemistry; D011247:Pregnancy; D012756:Sheep; D013569:Synapses / Q000187:drug effects*; D016708:Synaptophysin / Q000378:metabolism
D005938:Glucocorticoids; D016708:Synaptophysin; D001623:Betamethasone; D006854:Hydrocortisone