Comparison of histidine-tryptophan-ketoglutarate and Euro-Collins solutions for lung preservation using the minipig in situ warm ischemia model.
OBJECTIVE
Reperfusion injury remains a common problem in lung transplantation. This study compared the effect of lung preservation with histidine-tryptophan-ketoglutarate (HTK), a relatively low potassium solution, and Euro-Collins (EC) solution by using a minipig in situ model of warm ischemia.


METHODS
The left lungs of 5 minipigs were selectively flushed with EC solution. HTK was used for flush perfusion in 6 other minipigs. After 60 minutes of warm ischemia, the left lungs were reperfused. Hemodynamics, aerodynamics, and arterial blood gas were measured after the blood flow and ventilation of the contralateral lungs were temporally blocked. Bronchoalveolar lavage fluid (BALF) analysis, lung wet-to-dry weight ratio (W/D ratio) and histological analysis were done before perfusion (right lung) and 2 hours after reperfusion (left lung).


RESULTS
This in situ model of warm ischemia induced significantly increased pulmonary arterial pressure, pulmonary vascular resistance, BALF albumin contents and neutrophil counts, histological injury score, lung W/D ratio and tissue myeloperoxidase assay. The HTK perfusion group had a significantly lower degree of lung injury than the EC perfusion group.


CONCLUSIONS
Lung preservation with HTK solution resulted in better lung function after reperfusion than preservation by EC solution in this minipig model.
Luh
Shi-Ping
SP
Department of Surgery, Chung-Shan Medical University Hospital, Taichung, Taiwan.
Yang
Pan-Chyr
PC
Lee
Chun-Jean
CJ
Tsai
Tsung-Po
TP
Wang
Yao-Hong
YH
D000818:Animals; D005947:Glucose / Q000494:pharmacology*; D006982:Hypertonic Solutions / Q000494:pharmacology*; D008168:Lung; D016040:Lung Transplantation; D008353:Mannitol / Q000494:pharmacology*; D023421:Models, Animal; D009926:Organ Preservation; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology*; D011343:Procaine / Q000494:pharmacology*; D015427:Reperfusion Injury / Q000517:prevention & control; D013552:Swine; D013556:Swine, Miniature
C039984:Bretschneider cardioplegic solution; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D019188:Organ Preservation Solutions; D008353:Mannitol; D011343:Procaine; D011189:Potassium Chloride; D005947:Glucose