title: [Expression of neuropeptide Y in rat testes and its significance].
abstract: OBJECTIVE
  To assay the expression of neuropeptide Y (NPY) in rat testes and to investigate the significance of NPY in the regulation of androgen production and spermatogenesis.
  
  
  METHODS
  NPY mRNA levels in SD rat testes were measured by RT-PCR semi-quantity with beta-actin as internal control. NPY distribution was observed immunohistochemically.
  
  
  RESULTS
  NPY gene expressed in the testes, showing the strong positive band in the PCR production electrophoresis gel. In the immunostaining slides, NPY was found positively expressed in the Leyding cell area and around the testicular vessels and tubules, but not in the seminiferous tubules.
  
  
  CONCLUSIONS
  There was positive expression of NPY in the rat testes, which showed that NPY played a direct role in the regulation of testicular function.
authors.affiliation: Key Laboratory, First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Wang
authors.forename: Yugui
authors.initials: Y
authors.lastname: Cui
authors.forename: Ziqing
authors.initials: Z
authors.lastname: Chen
authors.forename: Qinqin
authors.initials: Q
authors.lastname: Pan
mesh_terms: D000818:Animals; D007150:Immunohistochemistry; D008297:Male; D009478:Neuropeptide Y / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000737:chemistry*
chemical_list: D009478:Neuropeptide Y; D012333:RNA, Messenger