title: iguana encodes a novel zinc-finger protein with coiled-coil domains essential for Hedgehog signal transduction in the zebrafish embryo.
abstract: Signaling by lipid-modified secreted glycoproteins of the Hedgehog family play fundamental roles during pattern formation in animal development and in humans; dysfunction of Hedgehog pathway components is frequently associated with a variety of congenital abnormalities and cancer. Transcriptional regulation of Hedgehog target genes is mediated by members of the Gli zinc-finger transcription factors. The relative nuclear concentrations of Gli activator (Gli(act)) and repressor (Gli(rep)) forms, together with their nucleocytoplasmic trafficking, appear to be critical determinants for target gene expression. Whereas such stringent controls of Gli activity are critical in ensuring appropriate levels of pathway activation, the mechanisms by which these processes are regulated remain inadequately understood. Here, using genetic analysis, we show that the zebrafish iguana gene product acts downstream of the Smoothened protein to modulate Gli activity in the somites of the developing embryo. Positional cloning reveals that iguana encodes the zebrafish ortholog of Dzip1, a novel zinc-finger/coiled-coil domain protein that we show can shuttle between the cytoplasm and nucleus in a manner correlated with Hedgehog pathway activity.
authors.affiliation: Center for Developmental Genetics, School of Medicine and Biomedical Sciences, University of Sheffield, Western Bank, Sheffield S10 2TN, UK.
authors.forename: Christian
authors.initials: C
authors.lastname: Wolff
authors.forename: Sudipto
authors.initials: S
authors.lastname: Roy
authors.forename: Katharine E
authors.initials: KE
authors.lastname: Lewis
authors.forename: Heike
authors.initials: H
authors.lastname: Schauerte
authors.forename: Gerd
authors.initials: G
authors.lastname: Joerg-Rauch
authors.forename: Annette
authors.initials: A
authors.lastname: Kirn
authors.forename: Christian
authors.initials: C
authors.lastname: Weiler
authors.forename: Robert
authors.initials: R
authors.lastname: Geisler
authors.forename: Pascal
authors.initials: P
authors.lastname: Haffter
authors.forename: Philip W
authors.initials: PW
authors.lastname: Ingham
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D029721:Drosophila Proteins; D004625:Embryo, Nonmammalian; D005260:Female; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins; D008969:Molecular Sequence Data; D009154:Mutation; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D000072086:Smoothened Receptor; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*; D000071676:Zinc Finger Protein GLI1; D000075746:Zinc Finger Protein Gli2; D016335:Zinc Fingers
chemical_list: D002352:Carrier Proteins; D029721:Drosophila Proteins; C488008:Dzip1 protein, zebrafish; D053823:Hedgehog Proteins; D015513:Oncogene Proteins; D043562:Receptors, G-Protein-Coupled; D012097:Repressor Proteins; D000072086:Smoothened Receptor; D015534:Trans-Activators; D014157:Transcription Factors; D029961:Zebrafish Proteins; D000071676:Zinc Finger Protein GLI1; D000075746:Zinc Finger Protein Gli2; C118355:gli2a protein, zebrafish; C100664:smo protein, Drosophila; C491021:smo protein, zebrafish; D017868:Cyclic AMP-Dependent Protein Kinases