title: Analysis of chromosomes 3, 7, X and the EGFR gene in uterine cervical cancer progression.
abstract: The aim of this study was to investigate the possible role of genetic alterations in the genesis and progression of cervical carcinomas. We analysed the 3, 7, X aneusomy of chromosomes and the status of the epidermal growth factor receptor (EGFR) gene by fluorescence in situ hybridisation (FISH) analysis. Polysomy of chromosomes 3 and X defined the transition from high-grade squamous intraepithelium lesions (HSIL) to cervical carcinoma. Chromosome 7 monosomy and polysomy did not show any statistical significant differences between the groups examined. When we compared the chromosomal aneusomies in all of the specimens using the Kruskal-Wallis test, significant differences (P = 0.0001, P = 0.0001 for chromosomes 3 and X, respectively) were observed. Using a ratio of the EGFR gene signals and chromosome 7 centromeric signals, no samples showed gene amplification. Our results demonstrate the importance of chromosomal 3 and X aneusomies in the development and progression from HSIL to cervical carcinoma, highlighting their usefulness as genetic markers for identifying SILs at high-risk of progression.
authors.affiliation: Department of Clinical Pathology (Cytogenetic Unit), Regina Elena Cancer Institute, 00144 Rome, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Marzano
authors.forename: G
authors.initials: G
authors.lastname: Corrado
authors.forename: R
authors.initials: R
authors.lastname: Merola
authors.forename: C
authors.initials: C
authors.lastname: Sbiroli
authors.forename: F
authors.initials: F
authors.lastname: Guadagni
authors.forename: E
authors.initials: E
authors.lastname: Vizza
authors.forename: F
authors.initials: F
authors.lastname: Del Nonno
authors.forename: M
authors.initials: M
authors.lastname: Carosi
authors.forename: M
authors.initials: M
authors.lastname: Galati M
authors.forename: I
authors.initials: I
authors.lastname: Sperduti
authors.forename: A M
authors.initials: AM
authors.lastname: Cianciulli
mesh_terms: D000328:Adult; D000368:Aged; D002869:Chromosome Aberrations; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics*; D041321:Chromosomes, Human, X / Q000235:genetics*; D018450:Disease Progression; D066246:ErbB Receptors / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008875:Middle Aged; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D066246:ErbB Receptors