Quantitative determination of curcuminoids in Curcuma rhizomes and rapid differentiation of Curcuma domestica Val. and Curcuma xanthorrhiza Roxb. by capillary electrophoresis.
The three major curcuminoids, curcumin, demethoxycurcumin and bis-demethoxycurcumin, from Curcuma domestica Val. (Curcuma longa L.) and Curcuma xanthorrhiza Roxb. (Zingiberaceae) were fully separated and quantified in less than 5 min using a capillary zone electrophoresis method with standard fused-silica capillaries and photodiode array detection. An electrolyte solution of 20 mM phosphate, 50 mM sodium hydroxide and 14 mM beta-cyclodextrin was found to be appropriate. Quantification was performed with 3,4-dimethoxy-trans-cinnamic acid as internal standard, and the limit of detection was 0.01 mg/mL. Extraction, stabilisation during sample storage and quantification procedures were optimised and carried out with drugs and commercial curry powder from different provenances. The results were compared with the photometric method of the monograph Curcumae xanthorrhizae rhizoma of the European Pharmacopoeia.
Lechtenberg
Matthias
M
Institute of Pharmaceutical Biology and Phytochemistry, Hittorfstrasse 56, D-48149 Münster, Germany.
Quandt
Bettina
B
Nahrstedt
Adolf
A
D030024:Curcuma / Q000737:chemistry*; D003474:Curcumin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000302:isolation & purification; D036381:Diarylheptanoids; D019075:Electrophoresis, Capillary / Q000379:methods*; D010783:Photometry; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry
D036381:Diarylheptanoids; D003474:Curcumin; C050229:demethoxycurcumin