Heterogeneous nuclear ribonucleoprotein A2/B1 identified as an autoantigen in autoimmune hepatitis by proteome analysis.
Our aim was to characterize antinuclear antibody (ANA) targets in type 1 autoimmune hepatitis (AIH1) using a proteomic tool. ANA-positive sera from 29 patients with AIH1 and 14 negative controls were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The principal antigens identified were a 30 kDa triplet band which was recognized by 79% of patient sera and 14% of control sera, and a 36 kDa antigen which was recognized by 52% and 14% of sera, respectively. The latter antigen was studied in more detail using two-dimensional electrophoresis (2-DE) and mass spectrometry (MS). Its identification corresponded to heterogeneous nuclear ribonucleoprotein A2/B1. This paper presents the first description of this protein as an antigen in AIH1.
Huguet
Stèphanie
S
Service d'Immunologie et Hématologie biologiques, Hôpital Saint-Antoine, Paris, France.
Labas
Valèrie
V
Duclos-Vallee
Jean-Charles
JC
Bruneel
Arnaud
A
Vinh
Joëlle
J
Samuel
Didier
D
Johanet
Catherine
C
Ballot
Eric
E
D000293:Adolescent; D000328:Adult; D000368:Aged; D000974:Antibodies, Antinuclear / Q000032:analysis; D001324:Autoantigens / Q000276:immunology*; D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005602:France; D019693:Hepatitis, Autoimmune / Q000276:immunology*; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D006801:Humans; D015151:Immunoblotting; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D008970:Molecular Weight; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D016622:Silver Staining; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000974:Antibodies, Antinuclear; D001324:Autoantigens; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D020543:Proteome