title: Transcriptional regulation of urokinase receptor in high- (95D) and low-metastatic (95C) human lung cancer cells.
abstract: To study the transcriptional regulation of urokinase receptor (uPAR) in high- (95D) and low-metastatic (95C) human lung cancer cells, we performed PCR to amplify 2238 bp uPAR promoter from 95C and 95D cells. According to the results of sequencing, five different bases are found in uPAR promoter between 95C and 95D cells. The results of luciferase activity assay showed that these differences have no significant effect on the uPAR promoter activity. Based on a normal uPAR promoter, progressive truncated mutants were constructed. The transient transfection/reporter assay showed that the promoter region from -136 to +9 may interact with relevant nuclear factors, which result in different levels of uPAR expression between 95C and 95D cells.
authors.affiliation: Key Laboratory, the Education Ministry, Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
authors.forename: Chun-Yang
authors.initials: CY
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Tan
authors.forename: Guo-Jing
authors.initials: GJ
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Chang
authors.initials: C
authors.lastname: Tong
authors.forename: Jing
authors.initials: J
authors.lastname: Fan
authors.forename: Yun-Song
authors.initials: YS
authors.lastname: Zhu
mesh_terms: D001483:Base Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002874:Chromosome Mapping; D003094:Collagen / Q000378:metabolism / Q000494:pharmacology; D004338:Drug Combinations; D017353:Gene Deletion; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006801:Humans; D007797:Laminin / Q000378:metabolism / Q000494:pharmacology; D008156:Luciferases / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011401:Promoter Regions, Genetic; D011509:Proteoglycans / Q000378:metabolism / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D055293:Receptors, Urokinase Plasminogen Activator; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D004338:Drug Combinations; D007797:Laminin; C524898:PLAUR protein, human; D011509:Proteoglycans; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; C059485:matrigel; D003094:Collagen; D008156:Luciferases