title: Evaluation of the methicillin-resistant Staphylococcus aureus (MRSA)-Screen latex agglutination test for detection of MRSA of animal origin.
abstract: Methicillin (oxacillin)-resistant staphylococci (MRS) have emerged as major clinical and epidemiological pathogens, and there have been frequent reports of MRS infections in the veterinary field. The MRSA-Screen latex agglutination test (Denka Seiken Co., Ltd., Tokyo, Japan) was compared with an oxacillin agar screen test, MIC determination, and mecA PCR assay, the "gold standard." In an analysis of 15 mecA-positive and 48 mecA-negative S. aureus animal isolates, as well as 9 mecA-positive and 147 mecA-negative, coagulase-negative staphylococcal animal isolates, the latex agglutination test surpassed the widely used oxacillin agar screen method and MIC determination, with a sensitivity and a specificity of 100%. The MRSA-Screen test is a reliable and rapid method of detecting MRS in the veterinary field.
authors.affiliation: College of Veterinary Medicine, Chonbuk National University, Chonju 561-756, South Korea. johnhlee@chonbuk.ac.kr
authors.forename: John H
authors.initials: JH
authors.lastname: Lee
authors.forename: Jae-Myung
authors.initials: JM
authors.lastname: Jeong
authors.forename: Yong-Ho
authors.initials: YH
authors.lastname: Park
authors.forename: Sung-Sun
authors.initials: SS
authors.lastname: Choi
authors.forename: Yong-Hwan
authors.initials: YH
authors.lastname: Kim
authors.forename: Joon-Seok
authors.initials: JS
authors.lastname: Chae
authors.forename: Jin-San
authors.initials: JS
authors.lastname: Moon
authors.forename: Hyun
authors.initials: H
authors.lastname: Park
authors.forename: Shin
authors.initials: S
authors.lastname: Kim
authors.forename: Seong-Kug
authors.initials: SK
authors.lastname: Eo
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D007841:Latex Fixation Tests; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D013211:Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification*; D013552:Swine