Transcriptional regulation of urokinase receptor in high- (95D) and low-metastatic (95C) human lung cancer cells.
To study the transcriptional regulation of urokinase receptor (uPAR) in high- (95D) and low-metastatic (95C) human lung cancer cells, we performed PCR to amplify 2238 bp uPAR promoter from 95C and 95D cells. According to the results of sequencing, five different bases are found in uPAR promoter between 95C and 95D cells. The results of luciferase activity assay showed that these differences have no significant effect on the uPAR promoter activity. Based on a normal uPAR promoter, progressive truncated mutants were constructed. The transient transfection/reporter assay showed that the promoter region from -136 to +9 may interact with relevant nuclear factors, which result in different levels of uPAR expression between 95C and 95D cells.
Li
Chun-Yang
CY
Key Laboratory, the Education Ministry, Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Tan
Li
L
Zhang
Guo-Jing
GJ
Li
Ping
P
Tong
Chang
C
Fan
Jing
J
Zhu
Yun-Song
YS
D001483:Base Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002874:Chromosome Mapping; D003094:Collagen / Q000378:metabolism / Q000494:pharmacology; D004338:Drug Combinations; D017353:Gene Deletion; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006801:Humans; D007797:Laminin / Q000378:metabolism / Q000494:pharmacology; D008156:Luciferases / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011401:Promoter Regions, Genetic; D011509:Proteoglycans / Q000378:metabolism / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D055293:Receptors, Urokinase Plasminogen Activator; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014162:Transfection
D004338:Drug Combinations; D007797:Laminin; C524898:PLAUR protein, human; D011509:Proteoglycans; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; C059485:matrigel; D003094:Collagen; D008156:Luciferases