title: Isolation of high quality RNA from Streptomyces.
abstract: Several molecular techniques require high quality RNA, completely free of DNA. Standard methods to isolate total RNA from Streptomyces spp. are based on the application of a 'Modified Kirby Mix' [Practical Streptomyces Genetics. The John Innes Foundation, Norwich, pp. 613]. Here we present an alternative procedure using Triton X-100 and EDTA for the isolation of total RNA from Streptomyces.
authors.affiliation: Laboratory of Bacteriology, Rega Institute, K.U.Leuven, 10 Minderbroedersstraat, B-3000 Leuven, Belgium.
authors.forename: W
authors.initials: W
authors.lastname: Van Dessel
authors.forename: L
authors.initials: L
authors.lastname: Van Mellaert
authors.forename: N
authors.initials: N
authors.lastname: Geukens
authors.forename: E
authors.initials: E
authors.lastname: Lammertyn
authors.forename: J
authors.initials: J
authors.lastname: Anné
mesh_terms: D004492:Edetic Acid / Q000737:chemistry; D006146:Guanidines / Q000737:chemistry; D017830:Octoxynol / Q000737:chemistry; D010636:Phenols / Q000737:chemistry; D012329:RNA, Bacterial / Q000302:isolation & purification*; D013302:Streptomyces / Q000737:chemistry / Q000235:genetics*
chemical_list: D006146:Guanidines; D010636:Phenols; D012329:RNA, Bacterial; C411644:trizol; D017830:Octoxynol; D004492:Edetic Acid