Macrolide resistance phenotypes of commensal viridans group streptococci and Gemella spp. and PCR detection of resistance genes.
One hundred and sixty viridans group streptococci (VGS) and 26 Gemella spp. resistant to erythromycin were studied to detect macrolide lincosamide and streptogramin B (MLS(B)) phenotypes and to investigate resistance rates to other antibiotics. The M phenotype was most prevalent in both bacterial groups (59.6% in VGS, 69.2% in gemellae) and the iMLS(B) phenotype was found least often (9.3 and 13.9%, respectively). All isolates with M phenotype had the mef(A/E) gene, being prevalent the mef(E) subclass. cMLS(B) and iMLS(B) strains contained the erm(B) gene, alone or in combination with the mef(A/E) gene. Thirteen isolates were intermediately resistant to quinupristin/dalfopristin and 11 strains showed low susceptibility to telithromycin. Linezolid was active against all the isolates tested and tetracycline resistance was the major one in VGS (41.6%) and Gemella spp. (46.2%).
Cerdá Zolezzi
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Department of Microbiology, Faculty of Medicine, Clinical University Hospital "Lozano Blesa", University of Zaragoza, c/Domingo Miral s/n, 50009 Zaragoza, Spain.
Rubio Calvo
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MC
Millán
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Goñi
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Canales
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M
Capilla
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Durán
E
E
Gómez-Lus
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D000081:Acetamides / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D004917:Erythromycin / Q000494:pharmacology; D005798:Genes, Bacterial; D006801:Humans; D048628:Ketolides / Q000494:pharmacology; D055231:Lincosamides; D000069349:Linezolid; D018942:Macrolides / Q000494:pharmacology*; D008565:Membrane Proteins / Q000235:genetics; D008780:Methyltransferases / Q000235:genetics; D008826:Microbial Sensitivity Tests; D023303:Oxazolidinones / Q000494:pharmacology; D040141:Staphylococcaceae / Q000187:drug effects* / Q000235:genetics*; D025381:Streptogramin B / Q000494:pharmacology; D013753:Tetracycline Resistance; D014769:Virginiamycin / Q000494:pharmacology; D034363:Viridans Streptococci / Q000187:drug effects* / Q000235:genetics*
D000081:Acetamides; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D048628:Ketolides; D055231:Lincosamides; D018942:Macrolides; C117866:MefA protein, Streptococcus; D008565:Membrane Proteins; D023303:Oxazolidinones; D014769:Virginiamycin; C062940:quinupristin-dalfopristin; D025381:Streptogramin B; D004917:Erythromycin; D008780:Methyltransferases; C035181:rRNA (adenosine-O-2'-)methyltransferase; D000069349:Linezolid; C106791:telithromycin