title: [Serological screening of xenogeneic antigens from rat testis].
abstract: OBJECTIVE
  It is the intent of this study to analyzed the rat testis library by employing a modified SEREX (serological analysis of recombinant cDNA expression library) approach to find the xenogeneic homologous tumor antigens which could be useful in developing cancer vaccines.
  
  
  METHODS
  The screening serum was obtained from the immunized rabbits with human ovarian cancer cells, and 10 positive clones were isolated from the rat testis cDNA library using SEREX technology.
  
  
  RESULTS
  We found these 10 clones encoded seven different proteins by means of bioinformatic analysis. Among them, OV-2 and OV-4 encode proteins related to carcinoma in human, yet OV-6 and OV-7 are novel and their encoded proteins remain unknown.
  
  
  CONCLUSIONS
  This study indicates that the utilization of xenogeneic immunized serum in the serological screening of xenogeneic homologous tumor antigens may expand the application of traditional SEREX technology.
authors.affiliation: Key Laboratory of Biotherapy of Human Diseases of Ministry of Education, Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Peng
authors.forename: Ling
authors.initials: L
authors.lastname: Tian
authors.forename: Hong-xin
authors.initials: HX
authors.lastname: Deng
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Han-shuo
authors.initials: HS
authors.lastname: Yang
authors.forename: Bing
authors.initials: B
authors.lastname: Kan
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.forename: Jian
authors.initials: J
authors.lastname: Liao
authors.forename: Yu-quan
authors.initials: YQ
authors.lastname: Wei
mesh_terms: D000818:Animals; D015478:Antigens, Heterophile / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000276:immunology; D001943:Breast Neoplasms / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics; D051381:Rats; D011994:Recombinant Proteins / Q000276:immunology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012698:Serologic Tests / Q000379:methods; D013737:Testis / Q000276:immunology*
chemical_list: D015478:Antigens, Heterophile; D000951:Antigens, Neoplasm; D018076:DNA, Complementary; D009363:Neoplasm Proteins; D011994:Recombinant Proteins