Molecular typing and phylogenetic analysis of enteroinvasive Escherichia coli using the fliC gene sequence.
Non-motile enteroinvasive Escherichia coli (EIEC) is serotyped based only on O antigen polymorphism, since H antigen epitopes, present on the flagellins, cannot be characterised in these bacteria. In this study, we demonstrate the presence of the flagellin-coding fliC gene in non-motile EIEC strains. Moreover, we were able to group the 11 most common non-motile EIEC serotypes into six different RFLP patterns of the fliC gene. Amplicons representing each RFLP pattern were sequenced. Sequencing data were used to construct a phylogenetic tree which showed two main clusters: one sharing similarity with Shigella dysenteriae and pathogenic E. coli, and the other being closer to non-pathogenic E. coli.
Amhaz
Juliana M K
JM
Departamento de Anàlises Clínicas e Toxicológicas da FCF-USP, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo (USP), Av. Prof. Lineu Prestes 580, 05508-900 São Paulo, SP, Brazil.
Andrade
Agda
A
Bando
Silvia Y
SY
Tanaka
Tânia L
TL
Moreira-Filho
Carlos A
CA
Martinez
Marina B
MB
D015373:Bacterial Typing Techniques; D015252:Deoxyribonucleases, Type II Site-Specific; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology; D005408:Flagellin / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping
D005408:Flagellin; D015252:Deoxyribonucleases, Type II Site-Specific; C040158:GTAC-specific type II deoxyribonucleases