[Relationship of variation 3057 G-->A of exon 20 of leptin receptor gene to lipid metabolism and fat distribution of children with obesity].
OBJECTIVE
To assess the relationship of the variation of exon 20 of leptin receptor (LEPR) gene to the lipid metabolism and fat distribution of the children with obesity.


METHODS
Polymerase chain reaction-restriction fragment length polymorphism(RFLP) and polyacrylamide gel electrophoresis were used to analyze the variation of exon 20 of the LEPR gene of the obesity group(72 obesity children) and the control group(60 healthy children). At the same time, all childrens' serum triglyceride(TG),total cholesterol(TC),high density lipoprotein cholesterol(HDL-C), low density lipoprotein cholesterol(LDL-C), height and weight were measured, and their body mass index(BMI) and fat percent(%fat) were calculated.


RESULTS
Three genotypes of exon 20 of LEPR gene were detected in this study. Compared with the control, the frequency of gene variation at 3057 nucleotide G-->A transversion was higher(P<0.05). The concentration of serum TG and the BMI and %fat of the A/A genotype obesity children were higher than those of the G/G genotype ones(P<0.01) but the level of serum HDL of the A/A children were lower than that of the G/G children (P<0.01). As to the G/A genotype children, only their serum TG level was higher than that of the G/G genotype ones(P<0.05).


CONCLUSIONS
The above findings indicated there were polymorphisms in the children with obesity, and those polymorphisms might remarkably affect their lipid metabolism and fat distribution.
Liu
Chang-yun
CY
Department of Pediatrics, Weifang Medical College, Weifang Shandong, 261031 PR China. changyun1@163.com
Wang
Yong-qin
YQ
Liu
Hong-ying
HY
Ji
Jia-fen
JF
Li
Wei-hua
WH
Bie
Hui-ling
HL
Li
Lan-xiu
LX
D000273:Adipose Tissue / Q000378:metabolism*; D000293:Adolescent; D001483:Base Sequence; D002648:Child; D005091:Exons; D005260:Female; D006801:Humans; D050356:Lipid Metabolism; D008297:Male; D008969:Molecular Sequence Data; D009765:Obesity / Q000235:genetics* / Q000378:metabolism; D011110:Polymorphism, Genetic; D011956:Receptors, Cell Surface / Q000235:genetics*; D054411:Receptors, Leptin; D017422:Sequence Analysis, DNA
C515448:LEPR protein, human; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin