title: Neurobehavioral evaluation of Reln-rl-orl mutant mice and correlations with cytochrome oxidase activity.
abstract: The Reln-rl-orl mutation is characterized by a marked deficit in cerebellar granule cell and Purkinje cell number as well as ectopias in cerebellum, hippocampus, and neocortex. By comparison to Balb/c controls, Reln-rl-orl mutants did not alternate spontaneously in a T-maze and were deficient for visuomotor guidance in a water maze. Despite cerebellar ataxia and motor coordination impairments on stationary beam, coat-hanger, and rotorod tests, the horizontal motor activity of Reln-rl-orl mutants was not reduced in an open-field. The elevated cytochrome oxidase (CO) activity in Purkinje cells and the reduced CO activity in the roof nuclei (interpositus and dentate) of the mutants were associated with poor performance on the small stationary beam. In addition, deficient CO activity of the granular layer of the motor cortex was associated with shorter latencies before falling from the larger stationary beam and a lower number of rears in the open-field. Conversely, elevated CO activity in the polymorphic layer of primary somatosensory cortex was congruent with higher latencies before falling from the same apparatus, indicating functional compensation.
authors.affiliation: Faculté de Médecine et de Pharmacie, Université de Rouen, 22 bld Gambetta, INSERM EMI 9906, Bâtiment de Recherche, Salle 1D18, 76183 Rouen Cedex, France. lalonde@epeire.univ-rouen.fr
authors.forename: R
authors.initials: R
authors.lastname: Lalonde
authors.forename: K
authors.initials: K
authors.lastname: Hayzoun
authors.forename: M
authors.initials: M
authors.lastname: Derer
authors.forename: J
authors.initials: J
authors.lastname: Mariani
authors.forename: C
authors.initials: C
authors.lastname: Strazielle
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D001835:Body Weight / Q000235:genetics; D001921:Brain / Q000166:cytology / Q000378:metabolism; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000502:physiology*; D003576:Electron Transport Complex IV / Q000378:metabolism* / Q000502:physiology; D005106:Exploratory Behavior / Q000502:physiology; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000502:physiology*; D008297:Male; D018782:Maze Learning / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008818:Mice, Neurologic Mutants / Q000235:genetics / Q000502:physiology*; D009043:Motor Activity / Q000502:physiology*; D009419:Nerve Tissue Proteins; D009474:Neurons / Q000378:metabolism; D011597:Psychomotor Performance / Q000502:physiology*; D011689:Purkinje Cells / Q000378:metabolism; D000091042:Reelin Protein; D012697:Serine Endopeptidases
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D000091042:Reelin Protein; D003576:Electron Transport Complex IV; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases