A short note on the nucleolar size and density in apoptotic leukemic granulocytic precursors (HL-60 cells).
The present study was undertaken to provide more information on the nucleolar size and density in mononuclear blastic granulocytic precursors represented by HL-60 cells the proliferation of which was blocked by photodynamic treatment (PDT) which induced apoptotic process without preceding terminal maturation. Both the nucleolar size and density did not change in apoptotic cells in comparison with controls. Thus, large and dense nucleoli in apoptotic cells are not necessarily related to the nucleolar biosynthetic or cell proliferation activity.
Smetana
Karel
K
Institute of Hematology and Blood Transfusion, Prague, Czech Republic. karel.smetana@uhkt.cz
Chan
Pui K
PK
Marinov
Yuri
Y
Soucek
Josef
J
Hrkal
Zbynek
Z
Busch
Harris
H
D000622:Aminolevulinic Acid / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002466:Cell Nucleolus / Q000737:chemistry / Q000187:drug effects / Q000473:pathology*; D006098:Granulocytes / Q000187:drug effects / Q000473:pathology*; D018922:HL-60 Cells / Q000187:drug effects / Q000473:pathology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008027:Light; D009697:Nucleolus Organizer Region / Q000737:chemistry / Q000187:drug effects; D017319:Photosensitizing Agents / Q000494:pharmacology; D016622:Silver Staining
D017319:Photosensitizing Agents; D000622:Aminolevulinic Acid