Enhancement of DNA vaccine potency in rhesus macaques by electroporation.
The potency of an HIV DNA vaccine was enhanced in rhesus macaques by in vivo electroporation, as judged by increased onset, magnitude and duration of antibody and cell-mediated immune responses against both components of a combination Gag and Env vaccine. These data demonstrate the utility of the electroporation technology for use in large animals.
Otten
Gillis
G
Vaccines Research, Chiron Corporation, 4560 Horton St., Mail Stop 4-3, Emeryville, CA 94068, USA.
Schaefer
Mary
M
Doe
Barbara
B
Liu
Hong
H
Srivastava
Indresh
I
zur Megede
Jan
J
O'Hagan
Derek
D
Donnelly
John
J
Widera
Georg
G
Rabussay
Dietmar
D
Lewis
Mark G
MG
Barnett
Susan
S
Ulmer
Jeffrey B
JB
D016915:AIDS Vaccines / Q000008:administration & dosage* / Q000276:immunology; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D018274:Electroporation / Q000379:methods*; D015683:Gene Products, gag / Q000276:immunology; D008253:Macaca mulatta; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology
D016915:AIDS Vaccines; D000914:Antibodies, Viral; D015683:Gene Products, gag; D019444:Vaccines, DNA; D014765:Viral Vaccines