Meeting maximum residue limits: an improved screening technique for the rapid detection of antimicrobial residues in animal food products.
A rapid, high-throughput antimicrobial screening assay was developed using either a physical fluid extraction or a solvent extraction technique coupled to the commercially available PremiTest. The solvent extraction approach was fully validated for a wide range of tissues and the fluid extraction approach partially validated for porcine muscle. Both procedures can detect a wide range of antimicrobial compounds at or below maximum residue limit concentrations. The use of a solvent extraction provides an enhanced test capable of detecting a wider range of drugs than the fluid extraction approach at or below half maximum residue limit levels in a variety of matrices. Biochemical methods for the class-specific identification of beta-lactams and sulphonamides following initial screening were developed and validated. The approach is a significant improvement on existing methodologies as a tool for residues monitoring in surveillance programmes.
Stead
S
S
Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK. s.stead@csl.gov.uk
Sharman
M
M
Tarbin
J A
JA
Gibson
E
E
Richmond
S
S
Stark
J
J
Geijp
E
E
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis; D000890:Anti-Infective Agents / Q000032:analysis*; D001431:Bacteriological Techniques / Q000379:methods; D002417:Cattle; D002645:Chickens; D004350:Drug Residues / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D001411:Geobacillus stearothermophilus; D007769:Lactams / Q000032:analysis; D008452:Maximum Allowable Concentration; D008460:Meat / Q000032:analysis*; D015203:Reproducibility of Results; D012474:Salmon; D012997:Solvents; D013449:Sulfonamides / Q000032:analysis; D013552:Swine
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D007769:Lactams; D012997:Solvents; D013449:Sulfonamides