title: The expression of COX2 protein induced by Lactobacillus rhamnosus GG, endotoxin and lipoteichoic acid in T84 epithelial cells.
abstract: OBJECTIVE
  Lactobacillus rhamnosus GG (L. rhamnosus GG) possess immunomodulatory effects in the host. In the present study, the effect of L. rhamnosus GG on cyclooxygenase 2 (COX2) expression and its pharmacological control was investigated in human T84 colon epithelial cells.
  
  
  RESULTS
  T84 cells were exposed to freeze-dried L. rhamnosus GG in vitro, and the expression of COX2 was detected by Western blot.
  
  
  CONCLUSIONS
  L. rhamnosus GG induced COX2 expression in a time- and concentration-dependent manner in T84 cells. COX2 expression was inhibited by tyrosine kinase inhibitor genistein (100 micromol l(-1)), p38 mitogen-activated protein kinase (MAP kinase) inhibitor (SB203580; 1 micromol l(-1)) and dexamethasone (10 micromol l(-1)), whereas inhibitors of p42/44 MAP kinase (PD98095; 10 micromol l(-1)), protein kinase C (Ro 31-8220; 1 micromol l(-1)) and transcription factor nuclear factor kappa B (NF-kappaB) [pyrrolidinedithiocarbamate (PDTC) 100 micromol l(-1)] had practically no effect.
  
  
  CONCLUSIONS
  The results provide novel information on the cellular mechanisms involved in the interaction between L. rhamnosus GG and colon epithelial cells.
authors.affiliation: The Immunopharmacological Research Group, University of Tampere, Medical School and Tampere University Hospital, Tampere, Finland.
authors.forename: R
authors.initials: R
authors.lastname: Korhonen
authors.forename: O
authors.initials: O
authors.lastname: Kosonen
authors.forename: R
authors.initials: R
authors.lastname: Korpela
authors.forename: E
authors.initials: E
authors.lastname: Moilanen
mesh_terms: D003106:Colon / Q000166:cytology / Q000201:enzymology; D051546:Cyclooxygenase 2; D004731:Endotoxins / Q000494:pharmacology*; D004790:Enzyme Induction; D004847:Epithelial Cells; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis*; D007778:Lactobacillus / Q000254:growth & development*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008565:Membrane Proteins; D019936:Probiotics; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis*; D013682:Teichoic Acids / Q000494:pharmacology*
chemical_list: D004731:Endotoxins; D007527:Isoenzymes; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D013682:Teichoic Acids; C009900:lipoteichoic acid; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases