ROS-related enzyme expressions in endothelial cells regulated by tea polyphenols.
OBJECTIVE
Elevation of reactive oxygen species (ROS), especially the level of superoxide is a key event in many forms of cardiovascular diseases. To study the mechanism of tea polyphenols against cardiovascular diseases, we observed the expressions of ROS-related enzymes in endothelial cells.


METHODS
Tea polyphenols were co-incubated with bovine carotid artery endothelial cells (BCAECs) in vitro and intracellular NADPH oxidase subunits p22phox and p67phox, SOD-1, and catalase protein were detected using Western blot method.


RESULTS
Tea polyphenols of 0.4 microg/mL and 4.0 microg/mL (from either green tea or black tea) down-regulated NADPH oxidase p22phox and p67phox expressions in a dose-negative manner (P < 0.05), and up-regulated the expressions of catalase (P < 0.05).


CONCLUSIONS
Tea polyphenols regulate the enzymes involved in ROS production and elimination in endothelial cells, and may be beneficial to the prevention of endothelial cell dysfunction and the development of cardiovascular diseases.
Ying
Chen-Jiang
CJ
Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, Hubei, China. yingchenjiang@yahoo.com
Sun
Xiu-Fa
XF
Zhang
Shu-Lin
SL
Zhang
Xi-Ping
XP
Mao
Li-Mei
LM
Zuo
Xue-Zhi
XZ
Yao
Ping
P
D000818:Animals; D028241:Camellia sinensis / Q000737:chemistry*; D002339:Carotid Arteries / Q000166:cytology; D002374:Catalase / Q000096:biosynthesis; D002417:Cattle; D002478:Cells, Cultured; D015536:Down-Regulation; D042783:Endothelial Cells / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D026901:Membrane Transport Proteins / Q000096:biosynthesis; D009252:NADPH Dehydrogenase / Q000096:biosynthesis; D019255:NADPH Oxidases; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010750:Phosphoproteins / Q000096:biosynthesis; D059808:Polyphenols; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000096:biosynthesis; D000072105:Superoxide Dismutase-1; D015854:Up-Regulation
D005419:Flavonoids; D026901:Membrane Transport Proteins; D010636:Phenols; D010750:Phosphoproteins; D059808:Polyphenols; D017382:Reactive Oxygen Species; C000606290:SOD1 protein, human; C079789:neutrophil cytosol factor 67K; D002374:Catalase; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D019255:NADPH Oxidases; C086986:CYBA protein, human; D009252:NADPH Dehydrogenase