[A comparison of the lipopolysaccharides and O-specific polysaccharides of Azospirillum brasilense Sp245 and its omegon-Km mutants KM018 and Km252].
The lipopolysaccharides (LPSs) extracted from the outer membrane of Azospirillum brasilense Sp245 and its Omegon-Km mutants KM018 and KM252 with a hot aqueous solution of phenol were found to differ in the content of carbohydrates, glucosamine, and total phosphorus and in the proportion of octadecenoic and hexadecanoic acids in the lipid moieties of the LPSs. The carbohydrate moieties of the LPSs were heterogeneous in charge. The analysis of the O-specific polysaccharides (O-PSs) of the mutants KM018 and KM252 by gas-liquid chromatography, IR spectroscopy, and NMR spectroscopy showed that they are composed of the same linear pentasugar repeating units-->2)-beta-D-Rhap-(1-->3)-alpha-D-Rhap-(1-->3)-alpha-D-Rhap-(1-->2)- alpha-D-Rhap-(1-->2)-alpha-D-Rhap-(1-->as the O-PSs of the parent strain Sp245. The reported differences in the biological activity of the LPSs of the parent and mutant strains can be due to their different chemical structure.
Fedonenko
Iu P
IuP
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410015 Russia.
Zdorovenko
E L
EL
Konnova
S A
SA
Ignatov
V V
VV
Shliakhtin
G V
GV
D015806:Azospirillum brasilense / Q000737:chemistry* / Q000235:genetics; D002241:Carbohydrates / Q000032:analysis; D002849:Chromatography, Gas; D005944:Glucosamine / Q000032:analysis; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009154:Mutation; D019081:O Antigens / Q000737:chemistry*; D010169:Palmitic Acids / Q000032:analysis; D010758:Phosphorus / Q000032:analysis; D013055:Spectrophotometry, Infrared; D013229:Stearic Acids / Q000032:analysis
C114874:5-octadecenoic acid; D002241:Carbohydrates; D008070:Lipopolysaccharides; D019081:O Antigens; D010169:Palmitic Acids; D013229:Stearic Acids; D010758:Phosphorus; C093821:hexadecenoic acid; D005944:Glucosamine