Biodistribution assessment of a lutetium(III) texaphyrin analogue in tumor-bearing mice using NIR Fourier-transform Raman spectroscopy.
The use of near-infrared (NIR)-excited Fourier-transform (FT) Raman spectroscopy as a technique for evaluating the extent of photosensitizer localization in tumor (human pancreatic adenocarcinomas)-bearing mice has been tested using lutetium(III) texaphyrin analogue Lu-T2B2Tex. The complex was injected subcutaneously in the form of three injections given during the course of 3 days. The kinetics of biodistribution were then followed over a time scale of 1-6 days. The NIR-FT-Raman spectra of tissue samples obtained from the xenographic tumor, muscle, heart, brain, liver, spleen, kidney and blood were recorded and used to identify the presence of Lu-T2B2Tex in these tissues. Five Raman sensitizer markers were used to estimate the relative content of Lu-T2B2Tex in tumor at various postinjection times. UV-Visible (Vis) absorption spectroscopic detection of this sensitizer in tissue extracts was applied as a conventional method. Both spectroscopic methods were in good agreement with each other and confirm that Lu-T2B2Tex localizes well in tumor tissue. Maximal drug content was observed 3 days after the final injection. This time delay seems to be optimal for tumor irradiation in photodynamic therapy.
Synytsya
Alla
A
Department of Analytical Chemistry, Institute of Chemical Technology, Prague, Czech Republic. alla.synytsya@vscht.cz
Král
Vladimír
V
Matejka
Pavel
P
Poucková
Pavla
P
Volka
Karel
K
Sessler
Jonathan L
JL
D000230:Adenocarcinoma / Q000378:metabolism*; D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008665:Metalloporphyrins / Q000493:pharmacokinetics*; D051379:Mice; D008815:Mice, Inbred Strains; D008819:Mice, Nude; D023421:Models, Animal; D015394:Molecular Structure; D009368:Neoplasm Transplantation; D009566:Nitrates / Q000493:pharmacokinetics*; D010190:Pancreatic Neoplasms; D017319:Photosensitizing Agents / Q000493:pharmacokinetics*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation / Q000379:methods*; D019265:Spectroscopy, Near-Infrared / Q000295:instrumentation / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D014018:Tissue Distribution
D008665:Metalloporphyrins; D009566:Nitrates; D017319:Photosensitizing Agents; C102825:motexafin lutetium