Effect of Cu(II) on the electrochemically initiated reaction of thiols with N, N-diethyl- p-phenylenediamine: methodology for the indirect voltammetric determination of Cu(II).
The effect of Cu(II) on the determination of homocysteine via its electrochemically initiated reaction with N, N-diethyl-p-phenylenediamine is examined. The presence of copper inhibited the detection process for homocysteine owing to a complexation reaction occurring. This provided an indirect route for the sensitive and selective determination of Cu(II), which produced a linear response over the range from 2.5 to 500 microM and a limit of detection of 2.5 microM. The detection pathway was examined in the presence of metallic and inorganic ions, with negligible interference observed.
Kershaw
Jessica A
JA
Physical and Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, OX1 3QZ, UK.
Nekrassova
Olga
O
Banks
Craig E
CE
Lawrence
Nathan S
NS
Compton
Richard G
RG
D003300:Copper / Q000032:analysis / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D006710:Homocysteine / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D010655:Phenylenediamines / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D010655:Phenylenediamines; D013438:Sulfhydryl Compounds; D006710:Homocysteine; D003300:Copper; C013935:N,N-diethyl 4-phenylenediamine