Phototrophic utilization of taurine by the purple nonsulfur bacteria Rhodopseudomonas palustris and Rhodobacter sphaeroides.
Taurine metabolism by two phototrophically grown purple nonsulfur bacteria enrichment isolates has been examined. Rhodopseudomonas palustris (strain Tau1) grows with taurine as a sole electron donor, sulfur and nitrogen source during photoautotrophic growth. Rhodobacter sphaeroides (strain Tau3) grows on the compound as sole electron donor, sulfur and nitrogen source, and partial carbon source, in the presence of CO(2) during photoheterotrophic growth. Both organisms utilize an inducible taurine-pyruvate aminotransferase and a sulfoacetaldehyde acetyltransferase. The products of this metabolism are bisulfite and acetyl phosphate. Bisulfite ultimately was oxidized to sulfate, but this was not an adequate source of electrons for photometabolism. Experiments using either [U-(14)C]taurine or (14)CO(2) demonstrated that Rb. sphaeroides Tau3 assimilated the carbon from approximately equimolar amounts of taurine and exogenous CO(2). The taurine-carbon assimilation was not diminished by excess non-radioactive bicarbonate. Malate synthase (but not isocitrate lyase) was induced in these taurine-grown cells. It is concluded that assimilation of taurine carbon occurs through an intermediate other than CO(2). Similar labelling experiments with Rp. palustris Tau1 determined that taurine is utilized only as an electron donor for the reduction of CO(2), which contributes all the cell carbon. Photoautotrophic metabolism was confirmed in this organism by the absence of either malate synthase or isocitrate lyase in taurine+CO(2)-grown cells. Culture collection strains of these two bacteria did not utilize taurine in these fashions.
Novak
Ryan T
RT
U-2131 Beach Hall, University of Connecticut, Storrs, CT 06269, USA.
Gritzer
Rachel F
RF
U-2131 Beach Hall, University of Connecticut, Storrs, CT 06269, USA.
Leadbetter
Edward R
ER
U-2131 Beach Hall, University of Connecticut, Storrs, CT 06269, USA.
Godchaux
Walter
W
U-2131 Beach Hall, University of Connecticut, Storrs, CT 06269, USA.
D001639:Bicarbonates / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes / Q000378:metabolism; D003470:Culture Media; D008027:Light; D010788:Photosynthesis; D012242:Rhodobacter sphaeroides / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D012241:Rhodopseudomonas / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism; D013654:Taurine / Q000378:metabolism*
D001639:Bicarbonates; D002250:Carbon Radioisotopes; D003470:Culture Media; D002245:Carbon Dioxide; D013654:Taurine; D013455:Sulfur