title: Rab11 regulates the recycling and lysosome targeting of beta2-adrenergic receptors.
abstract: The pericentriolar recycling endosome (RE) may be an alternative compartment through which some beta2-adrenergic receptors (beta2ARs) recycle from early endosomes to the cell surface during prolonged exposure to agonist. For the transferrin receptor, CXCR2, and the M4-muscarinic acetylcholine receptor, trafficking through the RE and receptor recycling is regulated by the small GTPase rab11. The precise role of the RE and rab11 in regulating the cellular trafficking of the beta2AR is not understood. We therefore monitored trafficking of beta2ARs in HEK293 cells following the modulation of rab11 activity. Expression of a rab11 mutant deficient in GTP binding (as a fusion between enhanced green fluorescent protein (EGFP) and the rab11S25N mutant) significantly slowed receptor recycling to the cell surface from dispersed transferrin-positive peripheral vesicles following a brief exposure to agonist. The agonist was applied at a time when receptors have undergone only one or two rounds of endocytosis and recycling. In cells overexpressing wild-type rab11, beta2ARs localized to a rab11-positive compartment and the rate of beta2AR recycling to the cell surface was reduced, but only after prolonged exposure to agonist and multiple rounds of receptor endocytosis and recycling. This effect was associated with impaired beta2AR trafficking to lysosomes and receptor proteolysis, whereas the sorting of low-density lipoprotein from transferrin-positive vesicles to late endosomes and lysosomes was not affected. These data highlight a pivotal role for rab11 in regulating the traffic of a G protein-coupled receptor at the level of the RE, where modulation of rab11 activity dictates the balance between receptor recycling and downregulation during prolonged exposure to agonist.
authors.affiliation: Department of Pediatrics, Baylor College of Medicine, 6621 Fannin, CCC 1040.00, Houston, TX 77030, USA.
authors.forename: Robert H
authors.initials: RH
authors.lastname: Moore
authors.forename: Ellen E
authors.initials: EE
authors.lastname: Millman
authors.forename: Estrella
authors.initials: E
authors.lastname: Alpizar-Foster
authors.forename: Wenping
authors.initials: W
authors.lastname: Dai
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Knoll
mesh_terms: D002460:Cell Line; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009154:Mutation; D043588:Receptor, Muscarinic M4 / Q000378:metabolism; D018343:Receptors, Adrenergic, beta-2 / Q000378:metabolism*; D023063:Receptors, Interleukin-8B / Q000378:metabolism; D013997:Time Factors; D014168:Transferrin / Q000378:metabolism; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D000099193:rab11 GTP-Binding Proteins
chemical_list: D049452:Green Fluorescent Proteins; D006160:Guanosine Triphosphate; D008077:Lipoproteins, LDL; D043588:Receptor, Muscarinic M4; D018343:Receptors, Adrenergic, beta-2; D023063:Receptors, Interleukin-8B; D014168:Transferrin; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins