Analysis of chromosomes 3, 7, X and the EGFR gene in uterine cervical cancer progression.
The aim of this study was to investigate the possible role of genetic alterations in the genesis and progression of cervical carcinomas. We analysed the 3, 7, X aneusomy of chromosomes and the status of the epidermal growth factor receptor (EGFR) gene by fluorescence in situ hybridisation (FISH) analysis. Polysomy of chromosomes 3 and X defined the transition from high-grade squamous intraepithelium lesions (HSIL) to cervical carcinoma. Chromosome 7 monosomy and polysomy did not show any statistical significant differences between the groups examined. When we compared the chromosomal aneusomies in all of the specimens using the Kruskal-Wallis test, significant differences (P = 0.0001, P = 0.0001 for chromosomes 3 and X, respectively) were observed. Using a ratio of the EGFR gene signals and chromosome 7 centromeric signals, no samples showed gene amplification. Our results demonstrate the importance of chromosomal 3 and X aneusomies in the development and progression from HSIL to cervical carcinoma, highlighting their usefulness as genetic markers for identifying SILs at high-risk of progression.
Marzano
R
R
Department of Clinical Pathology (Cytogenetic Unit), Regina Elena Cancer Institute, 00144 Rome, Italy.
Corrado
G
G
Merola
R
R
Sbiroli
C
C
Guadagni
F
F
Vizza
E
E
Del Nonno
F
F
Carosi
M
M
Galati M
M
M
Sperduti
I
I
Cianciulli
A M
AM
D000328:Adult; D000368:Aged; D002869:Chromosome Aberrations; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics*; D041321:Chromosomes, Human, X / Q000235:genetics*; D018450:Disease Progression; D066246:ErbB Receptors / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008875:Middle Aged; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
D005819:Genetic Markers; D066246:ErbB Receptors