Use of a commercial methylcellulose medium with and without recombinant bovine granulocyte colony-stimulating factor for culturing bovine bone marrow cells.
A commercial methylcellulose culture medium, with and without the addition of recombinant bovine granulocyte colony-stimulating factor (rbG-CSF), was utilized for culturing bovine bone marrow cells in a colony-forming unit assay. Bone marrow mononuclear cells were isolated and cultured in a commercial methylcellulose-based medium containing several recombinant human cytokines. Cultures were prepared with and without 100 ng/mL of rbG-CSF. The size and mean number of colonies per plate from culture days 3 to 9 were compared. We concluded that bovine bone marrow colony growth was supported by this culture medium. The addition of rbG-CSF yielded larger and more numerous colonies. There were significantly more colonies on day 3 (P < 0.001), day 4 (P < 0.001), and day 5 (P = 0.03) with rbG-CSF. Both culture media had the highest colony counts on day 5.
Keller
Sonya L
SL
Department of Pathobiology, University of Guelph, Guelph, Ontario N1G 2W1. skeller@uoguelph.ca
Wood
R Darren
RD
Jefferson
Barbara J
BJ
Jacobs
Robert M
RM
D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay / Q000379:methods / Q000662:veterinary; D003470:Culture Media / Q000737:chemistry*; D016207:Cytokines; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006410:Hematopoiesis / Q000187:drug effects; D006801:Humans; D008747:Methylcellulose / Q000494:pharmacology; D011994:Recombinant Proteins; D013997:Time Factors
D003470:Culture Media; D016207:Cytokines; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D008747:Methylcellulose