title: Catalase deficiency drastically affects gene expression induced by high light in Arabidopsis thaliana.
abstract: In plants, hydrogen peroxide (H(2)O(2)) plays a major signaling role in triggering both a defense response and cell death. Increased cellular H(2)O(2) levels and subsequent redox imbalances are managed at the production and scavenging levels. Because catalases are the major H(2)O(2) scavengers that remove the bulk of cellular H(2)O(2), altering their levels allows in planta modulation of H(2)O(2) concentrations. Reduced peroxisomal catalase activity increased sensitivity toward both ozone and photorespiratory H(2)O(2)-induced cell death in transgenic catalase-deficient Arabidopsis thaliana. These plants were used as a model system to build a comprehensive inventory of transcriptomic variations, which were triggered by photorespiratory H(2)O(2) induced by high-light (HL) irradiance. In addition to an H(2)O(2)-dependent and -independent type of transcriptional response during light stress, microarray analysis on both control and transgenic catalase-deficient plants, exposed to 0, 3, 8, and 23 h of HL, revealed several specific regulatory patterns of gene expression. Thus, photorespiratory H(2)O(2) has a direct impact on transcriptional programs in plants.
authors.affiliation: Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, Technologiepark 927, B-9052 Ghent, Belgium.
authors.forename: Steven
authors.initials: S
authors.lastname: Vandenabeele
authors.forename: Sandy
authors.initials: S
authors.lastname: Vanderauwera
authors.forename: Marnik
authors.initials: M
authors.lastname: Vuylsteke
authors.forename: Stephane
authors.initials: S
authors.lastname: Rombauts
authors.forename: Christian
authors.initials: C
authors.lastname: Langebartels
authors.forename: Harald K
authors.initials: HK
authors.lastname: Seidlitz
authors.forename: Marc
authors.initials: M
authors.lastname: Zabeau
authors.forename: Marc
authors.initials: M
authors.lastname: Van Montagu
authors.forename: Dirk
authors.initials: D
authors.lastname: Inzé
authors.forename: Frank
authors.initials: F
authors.lastname: Van Breusegem
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000528:radiation effects*; D029681:Arabidopsis Proteins; D016923:Cell Death / Q000528:radiation effects; D003470:Culture Media; D015870:Gene Expression / Q000528:radiation effects*; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008027:Light; D020411:Oligonucleotide Array Sequence Analysis; D010126:Ozone / Q000633:toxicity; D020675:Peroxisomes / Q000201:enzymology; D018515:Plant Leaves / Q000201:enzymology / Q000528:radiation effects; D030821:Plants, Genetically Modified; D011506:Proteins / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; D003470:Culture Media; D011506:Proteins; D010126:Ozone; D006861:Hydrogen Peroxide; C490276:CAT2 protein, Arabidopsis