Circular rapid amplification of cDNA ends for high-throughput extension cloning of partial genes.
The rapid amplification of cDNA ends (RACE) procedure is a widely used PCR-based method to clone the cDNA ends of mRNA transcripts. Current RACE methods often produce a high background of nonspecific PCR products, which can exclude the identification of the target cDNA of interest. We describe here an improved RACE procedure using circular cDNA templates and demonstrate the successful extension cloning of 4406 cDNAs.
Fu
Glenn K
GK
Incyte Corporation, Palo Alto, CA 94304, USA. gfu@incyte.com
Wang
Jonathan T
JT
Yang
Junming
J
Au-Young
Janice
J
Stuve
Laura L
LL
D003001:Cloning, Molecular / Q000379:methods*; D004270:DNA, Circular / Q000737:chemistry*; D018076:DNA, Complementary / Q000737:chemistry*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000737:chemistry*
D004270:DNA, Circular; D018076:DNA, Complementary; D012333:RNA, Messenger