title: Effects of Helicobacter pylori water extract on expression of endothelial adhesion molecules.
abstract: The present study investigated whether Helicobacter pylori water extract induces the upregulation of intercellular adhesion molecule-1, vascular cell adhesion molecule-1 and E-selectin on human umbilical vein endothelial cells, using an ELISA. The nature of the substances mediating this upregulation was also analyzed. H pylori water extract derived from type strain (NCTC 11637) significantly upregulated intercellular adhesion molecule-1, vascular cell adhesion molecule-1 and E-selectin to the same extent as interleukin-1. Treatments with extracts from clinical strains showed no significant increases in expression of these adhesion molecules. In a fractionation study, approximately 7 kDa fraction showed peak activity. This activity was tolerant to heating and trypsin digestion. These results indicate that H pylori water extract contains water-soluble, low-molecular, nonprotein substances which induce upregulation of adhesion molecules on human umbilical vein endothelial cells, suggesting that products of H pylori may elicit gastric mucosal inflammation by promoting expression of endothelial adhesion molecules.
authors.affiliation: Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Japan. nyoshida@koto.kpu-m.ac.jp
authors.forename: Norimasa
authors.initials: N
authors.lastname: Yoshida
authors.forename: Kyoichi
authors.initials: K
authors.lastname: Kassai
authors.forename: Hironobu
authors.initials: H
authors.lastname: Murase
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kokura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ichikawa
authors.forename: Yuji
authors.initials: Y
authors.lastname: Naito
authors.forename: Takeshi
authors.initials: T
authors.lastname: Okanoue
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Yoshikawa
mesh_terms: D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000187:drug effects; D002850:Chromatography, Gel; D019040:E-Selectin / Q000032:analysis / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D016480:Helicobacter pylori / Q000737:chemistry*; D006358:Hot Temperature; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000187:drug effects; D007375:Interleukin-1 / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000032:analysis; D008970:Molecular Weight; D012995:Solubility; D014357:Trypsin / Q000187:drug effects; D014471:Umbilical Veins / Q000166:cytology; D015854:Up-Regulation; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis / Q000187:drug effects; D014867:Water
chemical_list: D015815:Cell Adhesion Molecules; D019040:E-Selectin; D007375:Interleukin-1; D008070:Lipopolysaccharides; D019010:Vascular Cell Adhesion Molecule-1; D014867:Water; D018799:Intercellular Adhesion Molecule-1; D014357:Trypsin