Isolation of long-lived mutants in Caenorhabditis elegans using selection for resistance to juglone.
The accumulation of molecular damage from attack by reactive oxygen species is one cause of aging. Therefore, some mutant organisms showing increased resistance to reactive oxygen species should live longer. We show that selection for Caenorhabditis elegans mutants that are resistant to juglone, a reactive oxygen species-generating compound, leads to the identification of long-lived mutants. Indeed, four of six resistant mutants isolated were also long-lived. This study illustrates once more the strong relationship between oxidative stress and the aging processes.
de Castro
Edouard
E
Institute for Behavioral Genetics, University of Colorado at Boulder, 80309, USA.
Hegi de Castro
Sarah
S
Johnson
Thomas E
TE
D000375:Aging; D000818:Animals; D017173:Caenorhabditis elegans; D003433:Crosses, Genetic; D004251:DNA Transposable Elements; D004351:Drug Resistance; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005609:Free Radicals; D005821:Genetic Techniques; D009154:Mutation; D009285:Naphthoquinones / Q000494:pharmacology*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D017382:Reactive Oxygen Species; D013312:Stress, Physiological; D013997:Time Factors; D014466:Ultraviolet Rays
D004251:DNA Transposable Elements; D004791:Enzyme Inhibitors; D005609:Free Radicals; D009285:Naphthoquinones; D017382:Reactive Oxygen Species; D010100:Oxygen; C005134:juglone