title: Fluorescent labelled thiourea-bridged glycodendrons.
abstract: GlcNAc-coated glycodendrimers, which are polyvalent glycomimetics, display strong in vitro affinity for the rat natural killer cell protein-1A (NKR-P1A), a C-type lectin-like receptor of natural killer (NK) cells in rats, humans and some strains of mice. Administration of these compounds in vivo results in a substantial increase in the antitumour activity with involvement of the natural cell immunity. To clarify the in vitro and in vivo fate of these molecules, we synthesized labelled glycodendron analogues of the previously studied glycodendrimers. Labelling with fluorescent tags enabled the localization of the glycodendrons in white blood cells, tumours and other tissues by using different imaging techniques such as fluorescence and confocal microscopy. These studies are useful for probing the mechanism of action and fate of artificial ligands and the cell receptors involved.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídenská 1083, 142 20 Prague 4, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Krist
authors.forename: Luca
authors.initials: L
authors.lastname: Vannucci
authors.forename: Marek
authors.initials: M
authors.lastname: Kuzma
authors.forename: Petr
authors.initials: P
authors.lastname: Man
authors.forename: Kashinath
authors.initials: K
authors.lastname: Sadalapure
authors.forename: Anupama
authors.initials: A
authors.lastname: Patel
authors.forename: Karel
authors.initials: K
authors.lastname: Bezouska
authors.forename: Milan
authors.initials: M
authors.lastname: Pospísil
authors.forename: Ladislav
authors.initials: L
authors.lastname: Petrus
authors.forename: Thisbe K
authors.initials: TK
authors.lastname: Lindhorst
authors.forename: Vladimír
authors.initials: V
authors.lastname: Kren
mesh_terms: D000818:Animals; D005453:Fluorescence; D005690:Galactose / Q000737:chemistry / Q000378:metabolism; D006001:Glycoconjugates / Q000138:chemical synthesis / Q000737:chemistry*; D007694:Killer Cells, Natural / Q000166:cytology / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D018613:Microscopy, Confocal; D015394:Molecular Structure; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013890:Thiourea / Q000737:chemistry*
chemical_list: D006001:Glycoconjugates; D013890:Thiourea; D005690:Galactose