[The observation of the lymphocyte phenotype change in different lymphoid tissues of mice after intranasal immunization with bivalent Shigella vaccines].
OBJECTIVE
To observe the changes of lymphocyte phenotype in different lymphoid tissues of mice at various time after intranasal immunization with bivalent Shigella vaccines.


METHODS
BALB/c mice were randomly divided into three groups, 30 mice per group. Mice were intranasally immunized respectively with PBS, FSM-2117 or FS-5416 four times (bacterial number was sequentially 5x10(6), 1x10(7),4x10(7)and 4x10(7)CFU/mouse) with two week intervals. Lymphocytes in nasal associated lymphoid tissue (NALT), nasal passage(NP), spleen or Peyer's patch (PP) were isolated on the 7th,30th and 90th day after the last immunization. The phenotype of the lymphocytes was detected by FACS.


RESULTS
CD3(+) T cells in NALT, NP and PP increased significantly on the 7th day after the immunization, in which most were CD4(+)T cells. B220(+) cells and CD3(+) T cells increased notably in spleens of FSM-2117 group and FS-5416 group, respectively. The same phenotypic changes still maintained in the NALT, NP and spleen on the 30th day after immunization, but only present in NALT and NP on the 90th day.


CONCLUSIONS
Intranasal inoculation with the two bivalent Shigella vaccines can effectively induce immune responses in different mucosal sites and systematically which can durate for a long time and start to weaken from the tissues distal from nose to those proximal.
Shu
Cui-li
CL
Laboratory of Immunology,Military 302 Hospital, Beijing 100039, China.
Gao
Jie-ying
JY
Peng
Hong
H
Xu
Hui
H
Wang
Hua
H
Chen
Jie
J
Shi
Xin-fu
XF
D000281:Administration, Intranasal; D000818:Animals; D005260:Female; D007114:Immunization; D016130:Immunophenotyping; D051379:Mice; D008807:Mice, Inbred BALB C; D022281:Shigella Vaccines / Q000008:administration & dosage / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D022281:Shigella Vaccines