title: Toward fingerprinting cellular lipidomes directly from biological samples by two-dimensional electrospray ionization mass spectrometry.
abstract: Cellular lipids are composed of linear combinations of aliphatic chains covalently attached to backbones and/or head groups that collectively represent the cellular lipidome. Herein we present a novel two-dimensional electrospray ionization mass spectrometric approach to fingerprint most of the major and many of the minor lipid classes in the hepatic cellular lipidome, which collectively represent >80% of the total lipid mass, directly from their chloroform extracts. Through lipid class-selective intrasource ionization and subsequent analysis of two-dimensional cross-peak intensities, the chemical identity and mass composition of individual molecular species of most mouse hepatic lipid classes were determined from its chloroform extract. This new integrated platform provides a robust foundation for the automated analysis of multidimensional mass spectra to advance the level of analytical scripting in lipidomics to a new plateau.
authors.affiliation: Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St. Louis, MO 63110, USA. xianlin@wustl.edu
authors.forename: Xianlin
authors.initials: X
authors.lastname: Han
authors.forename: Jingyue
authors.initials: J
authors.lastname: Yang
authors.forename: Hua
authors.initials: H
authors.lastname: Cheng
authors.forename: Hongping
authors.initials: H
authors.lastname: Ye
authors.forename: Richard W
authors.initials: RW
authors.lastname: Gross
mesh_terms: D000818:Animals; D005591:Chemical Fractionation; D002725:Chloroform; D008055:Lipids / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D008099:Liver / Q000737:chemistry; D008297:Male; D051379:Mice; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D008055:Lipids; D002725:Chloroform