Isolation of differentially expressed genes from contaminant exposed European flounder by suppressive, subtractive hybridisation.
Commercially farmed European flounder (Platichthys flesus) were treated with benzo[a]pyrene or cadmium under controlled laboratory conditions. In addition, animals were collected from the polluted Tyne and relatively clean Alde estuaries in the UK. Potential differentially expressed genes were isolated from liver tissue by means of suppressive, subtractive hybridisation. A total of 284 different mRNAs were identified as having the potential for being differentially expressed, of which 84 could be tentatively identified by homology with database sequences. These clones will form part of a flounder cDNA array for validation and future applications, with full identification being pursued for those expressed sequence tags for which differential expression can be confirmed by array technology.
Sheader
Derek L
DL
School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. DLS648@bham.ac.uk
Gensberg
Karl
K
Lyons
Brett P
BP
Chipman
Kevin
K
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002104:Cadmium / Q000633:toxicity*; D030541:Databases, Genetic; D005432:Flounder / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D008099:Liver / Q000378:metabolism; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000302:isolation & purification* / Q000378:metabolism; D006113:United Kingdom
D012333:RNA, Messenger; D002104:Cadmium; D001564:Benzo(a)pyrene