Isolation and characterization of anti-HIV peptides from Dorstenia contrajerva and Treculia obovoidea.
Using a high throughput screen based on the interaction of the HIV-1 gp41 ectodomain with the virucidal protein cyanovirin-N (CV-N), we isolated two new peptides which inhibited the binding of CV-N to gp41 and which subsequently showed anti-HIV activity in a whole cell assay. A 5-kDa (contrajervin) and 10 kDa (treculavirin) peptide were isolated from Dorstenia contrajerva and Treculia obovoidea, respectively. Treculavirin was composed of two subunits, each containing 50 amino acid residues, which are covalently linked by at least one disulfide bond between the subunits. Both peptides were shown to bind to gp41 and gp120 and to inhibit the cytopathic effects of HIV-1(RF) infection in a human T-lymphoblastoid cell line (CEM-SS).
Bokesch
Heidi R
HR
Basic Research Program, SAIC-Frederick, and Molecular Targets Development Program, Center for Cancer Research, National Cancer Institute-Frederick, National Institutes of Health, MD 21702-1201, USA.
Charan
Romila D
RD
Meragelman
Karina M
KM
Beutler
John A
JA
Gardella
Roberta
R
O'Keefe
Barry R
BR
McKee
Tawnya C
TC
McMahon
James B
JB
D000117:Acetylglucosamine / Q000378:metabolism; D000595:Amino Acid Sequence; D019380:Anti-HIV Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D001426:Bacterial Proteins; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000378:metabolism; D002460:Cell Line; D005638:Fruit / Q000737:chemistry*; D015700:HIV Envelope Protein gp41 / Q000235:genetics / Q000378:metabolism; D015497:HIV-1 / Q000737:chemistry / Q000187:drug effects / Q000472:pathogenicity; D006801:Humans; D008358:Mannose / Q000378:metabolism; D008969:Molecular Sequence Data; D029586:Moraceae / Q000737:chemistry*; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013601:T-Lymphocytes / Q000166:cytology; D014994:Xylose / Q000378:metabolism
D019380:Anti-HIV Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D015700:HIV Envelope Protein gp41; D010940:Plant Proteins; D011994:Recombinant Proteins; C107733:cyanovirin N; D014994:Xylose; D008358:Mannose; D000117:Acetylglucosamine