Solubilization of aggregates formed by expanded polyglutamine tract expression in cultured cells.
The expression of expanded polyglutamine in mammalian cells causes the formation of aggregates. For elucidation of the biochemical properties of the aggregates, isolation and solubilization of the aggregates are required. This chapter provides useful protocols for the solubilization of polyglutamine aggregates and further applications of protein analysis.
Hazeki
Noriko
N
Division of Neuroscience, Department of Neurology, CREST, Japan Science and Technology Corporation, Graduate School of Medicine, University of Tokyo, Japan.
Kanazawa
Ichiro
I
D001483:Base Sequence; D002469:Cell Separation; D002478:Cells, Cultured; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins; D006801:Humans; D006816:Huntington Disease / Q000378:metabolism; D008164:Luminescent Proteins / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*
D017931:DNA Primers; D008164:Luminescent Proteins; D010455:Peptides; D049452:Green Fluorescent Proteins; C097188:polyglutamine