title: Progesterone determination in Iberian red deer by time-resolved fluorometry: an alternative method to RIA.
abstract: The validation for Iberian red deer of a commercially available Time-resolved fluoroimmunoassay (TR-FIA) designed for analysis of progesterone in human beings was carried out. Intra-assay coefficients of variation ranged from 3.6% to 7.4%, while inter-assay coefficients of variation varied from 5.2% to 15.5%. Accuracy, evaluated by comparing results yielded by TR-FIA with those obtained from a validated radioimmunoassay (RIA) in the measurement of 14 samples, provided a high regression coefficient (R(2)= 0.93). Different progesterone concentrations added to pool plasma showed percentages of recovery that ranged between 102.6% and 82.48%. The limit of detection was 0.102 nmol/L. The results obtained indicate that the present method is suitable for the measurement of progesterone in female Iberian red deer.
authors.affiliation: Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Murcia, 30100 Murcia, Spain.
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Parra
authors.forename: Andrés José
authors.initials: AJ
authors.lastname: García
authors.forename: Luis Jesús
authors.initials: LJ
authors.lastname: Bernal
authors.forename: Tomás
authors.initials: T
authors.lastname: Landete-Castillejos
authors.forename: José Joaquín
authors.initials: JJ
authors.lastname: Cerón
mesh_terms: D000818:Animals; D003670:Deer / Q000097:blood*; D005260:Female; D005470:Fluorometry / Q000379:methods; D011374:Progesterone / Q000097:blood*; D011863:Radioimmunoassay; D012044:Regression Analysis; D015203:Reproducibility of Results; D013030:Spain; D013997:Time Factors
chemical_list: D011374:Progesterone