title: Rapid detection of quinolone-resistant Salmonella by real time SNP genotyping.
abstract: A total of 63 isolates were screened for the gyrA mutation (87Asp-Tyr) in Salmonella enterica serovars using real time PCR. All of the isolates were successfully identified as resistant or susceptible, consistent with the MIC result of the agar dilution method and gyrA sequencing.
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1 Tokura, Kokubunji, Tokyo 185-8511, Japan.
authors.forename: Hidetake
authors.initials: H
authors.lastname: Esaki
authors.forename: Ken
authors.initials: K
authors.lastname: Noda
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Otsuki
authors.forename: Akemi
authors.initials: A
authors.lastname: Kojima
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Asai
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tamura
authors.forename: Toshio
authors.initials: T
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D027081:DNA Gyrase / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005456:Fluorescent Dyes / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D015363:Quinolones / Q000494:pharmacology*; D012481:Salmonella Infections, Animal / Q000382:microbiology; D019779:Salmonella enterica / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D005456:Fluorescent Dyes; D015363:Quinolones; D027081:DNA Gyrase