Improved use of organic phosphate by Skeletonema costatum through regulation of Zn2+ concentrations.
The maximum growth rate (1.4-2 x 10(5) cells ml(-1) d(-1)), cell final yields (2.6-5.2 x 10(5) cells ml(-1)) and extracellular alkaline phosphatase activity (2.4-10.6 microg phosphate released ml(-1) h(-1)) of the red tide alga, Skeletonema costatum, increased when Zn2+ was increased from 0 to 24 pM, but decreased with 66 pM Zn2+ in growth medium with glycerophosphate as the sole phosphorus source. Extracellular carbonic anhydrase activity and the affinity for HCO3- and CO2 uptake increased when Zn2+ was increased from 0 to 12 pM, but then decreased at higher concentrations. The results suggested that utilization of organic phosphate required more Zn2+ than the uptake of inorganic carbon did, while utilization of dissolved inorganic carbon by Skeletonema costatum was very sensitive to Zn2+ concentration variations.
Shi
Yanjun
Y
School of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
Hu
Hanhua
H
Ma
Runyu
R
Cong
Wei
W
Cai
Zhaoling
Z
D000469:Alkaline Phosphatase / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D002245:Carbon Dioxide / Q000737:chemistry; D002256:Carbonic Anhydrases / Q000378:metabolism; D049109:Cell Proliferation; D002734:Chlorophyll / Q000737:chemistry; D000077194:Chlorophyll A; D056890:Eukaryota / Q000378:metabolism*; D005994:Glycerophosphates / Q000737:chemistry; D007700:Kinetics; D010100:Oxygen / Q000378:metabolism; D010710:Phosphates / Q000737:chemistry*; D010788:Photosynthesis; D017693:Sodium Bicarbonate / Q000737:chemistry; D015032:Zinc / Q000737:chemistry*
D005994:Glycerophosphates; D010710:Phosphates; D002734:Chlorophyll; D002245:Carbon Dioxide; D002244:Carbon; D017693:Sodium Bicarbonate; D000469:Alkaline Phosphatase; D002256:Carbonic Anhydrases; D015032:Zinc; D010100:Oxygen; D000077194:Chlorophyll A