Chlorophyll and carotenoid degradation mediated by thylakoid-associated peroxidative activity in olives (Olea europaea) cv. hojiblanca.
A peroxidative activity was found in solubilized thylakoid membranes of olives (Olea europaea) cv. hojiblanca that catalyses degradation of chloroplast pigments in the presence of H2O2 and 2,4-dichlorophenol (DCP). The intermediate products of this degradation were analyzed using HPLC with diode array detection and the results indicated that 13(2)-OH-chlorophyll a and 13(2)-OH-chlorophyll b were the primary catabolites. The peroxidative activity assosiated with the thylakoid membranes affected, not only chlorophyll a and chlorophyll b, but also other accessory pigments in the photosynthetic process, such as the carotenoids. Quantitatively, the progressive decrease of the ratios Chl a/b and total Chls a+b/carotenoids indicated a more rapid disappearance of Chl a than of Chl b and a faster degradation of Chls a+b than of carotenoids.
Gandul-Rojas
Beatriz
B
Departamento de Biotecnologia de Alimentos, Instituto de la Grasa, CSIC, Av/Padre García Tejero, 4, Sevilla 41012, Spain.
Roca
María
M
Mínguez-Mosquera
María Isabel
MI
D002338:Carotenoids / Q000378:metabolism*; D002733:Chlorophenols / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism*; D000077194:Chlorophyll A; D002851:Chromatography, High Pressure Liquid; D006861:Hydrogen Peroxide / Q000378:metabolism; D031658:Olea / Q000737:chemistry / Q000378:metabolism*; D020524:Thylakoids / Q000737:chemistry / Q000378:metabolism*
D002733:Chlorophenols; D002734:Chlorophyll; D002338:Carotenoids; C037184:chlorophyll b; D006861:Hydrogen Peroxide; C004762:2,4-dichlorophenol; D000077194:Chlorophyll A