Individual-specific variation of gene expression in peripheral blood leukocytes.
DNA microarray technology is used to determine gene expression profiles of various cell types, especially abnormal cells, such as cancer. By contrast, relatively little attention has been given to expression profiling of normal tissues. Here we describe studies of gene expression in peripheral blood leukocytes (PBL) from normal individuals sampled multiple times over periods ranging from several weeks up to 6 months. We demonstrate stable patterns of gene expression that differ between individuals. Among the genes whose expression varies by individual is a group of genes responsive to interferon stimulation. Certain individuals ( approximately 10-20% of those tested) showed higher baseline levels and lower inducibility of these genes in response to in vitro interferon stimulation. These studies demonstrate the feasibility of using DNA microarrays to measure the variations in gene expression of PBL from different individuals in response to environmental and genetic factors.
Radich
Jerald P
JP
Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, D4-100, Seattle, WA 98109, USA. jradich@fhcrc.org
Mao
Mao
M
Stepaniants
Sergey
S
Biery
Matt
M
Castle
John
J
Ward
Terry
T
Schimmack
Greg
G
Kobayashi
Sumire
S
Carleton
Michael
M
Lampe
Johanna
J
Linsley
Peter S
PS
D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D014644:Genetic Variation; D006801:Humans; D007372:Interferons / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis
D008070:Lipopolysaccharides; D007372:Interferons