Expression of neutral glycosphingolipids in cytokine-stimulated human endothelial cells.
We compared immunohistochemical distribution of glycosphingolipids globotriosylceramide (GbOse(3)Cer) and globotetraosylceramide (GbOse(4)Cer) with that of E-selectin on human umbilical vein endothelial cells (HUVEC) stimulated with tumor necrosis factor (TNF)-alpha. HUVECs activated by TNF-alpha were characterized by the highest expression of E-selectin and greatest adhesion of HL-60 cells as well compared to stimulation with interleukin-1beta or lipopolysaccharide. HUVECs activated by TNF-alpha also stained intensely with globoside antibodies, especially with the GbOse(3)Cer-directed one, staining being redistributed in a concentration-dependent manner. These results indicate the possible role of GbOse(3)Cer and GbOse(4)Cer in immune effector mechanisms of endothelium such as adhesion.
Zemunik
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T
Department of Biology, School of Medicine, Split University, Split, 21000, Croatia. tzemunik@bsb.mefst.hr
Markotić
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Marusić
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A
D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D019040:E-Selectin / Q000378:metabolism*; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D005915:Globosides / Q000378:metabolism*; D018922:HL-60 Cells / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D007375:Interleukin-1; D008070:Lipopolysaccharides / Q000494:pharmacology; D014281:Trihexosylceramides / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology
D019040:E-Selectin; D005915:Globosides; D007375:Interleukin-1; D008070:Lipopolysaccharides; D014281:Trihexosylceramides; D014409:Tumor Necrosis Factor-alpha; C024032:globotetraosylceramide; C018549:globotriaosylceramide