title: [Development of human myeloid leukemia-like phenotype in NUP98-PMX1 transgenic mice].
abstract: OBJECTIVE
  In order to investigate the leukemogenic potential of NUP98-PMX1 fusion gene in vivo.
  
  
  METHODS
  NUP98-PMX1 transgenic mice were generated, in which the fusion gene was driven by hCG promoter and expressed in myeloid cells at early stage of differentiation. Molecular cloning technology was used to construct NUP98-PMX1 transgenic plasmid. The genotype and phenotype of the NUP98-PMX1 transgenic mice were analyzed by PCR, RT-PCR, peripheral blood count (PBC), bone marrow (BM) cells morphology and pathological examination.
  
  
  RESULTS
  NIH3T3 cells transfected with NUP98-PMX1 fusion gene grew faster, formed colonies in soft agar, and developed tumors in 10 inoculated nude mice. Among 8 disordered NUP98-PMX1 transgenic mice, 4 developed myeloid leukemia-like phenotype, including 3 resembling human chronic myeloid leukemia.
  
  
  CONCLUSIONS
  NUP98-PMX1 has oncogenic activity and plays a crucial role in leukemogenesis.
authors.affiliation: Lab of Medical & Molecular Genetics, Department of Medical Genetics, Shanghai Second Medical University, Shanghai 200025, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Ming-min
authors.initials: MM
authors.lastname: Gu
authors.forename: Yi
authors.initials: Y
authors.lastname: Tan
authors.forename: Shun-yuan
authors.initials: SY
authors.lastname: Lu
authors.forename: Long
authors.initials: L
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Kong
authors.forename: Yue-ping
authors.initials: YP
authors.lastname: Sun
authors.forename: Zhen-yu
authors.initials: ZY
authors.lastname: Lu
authors.forename: Sai-juan
authors.initials: SJ
authors.lastname: Chen
authors.forename: Zhen-yi
authors.initials: ZY
authors.lastname: Wang
authors.forename: Zhu-gang
authors.initials: ZG
authors.lastname: Wang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D015973:Gene Expression Regulation, Leukemic; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008819:Mice, Nude; D008822:Mice, Transgenic; D041681:NIH 3T3 Cells; D028861:Nuclear Pore Complex Proteins / Q000235:genetics* / Q000378:metabolism; D015514:Oncogene Proteins, Fusion / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: C120274:NUP98-PMX1 fusion protein, human; D028861:Nuclear Pore Complex Proteins; D015514:Oncogene Proteins, Fusion; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins