Quantitative analysis of cannabinoids from Cannabis sativa using 1H-NMR.
A (1)H-NMR method has been developed for the quantitative analysis of pure cannabinoids and for cannabinoids present in Cannabis sativa plant material without any chromatographic purification. The experiment was performed by the analysis of singlets in the range of delta 4.0-7.0 in the (1)H-NMR spectrum, in which distinguishable signals of each cannabinoid are shown. Quantitation was performed by calculating the relative ratio of the peak area of selected proton signals of the target compounds to the known amount of the internal standard, anthracene. For this method no reference compounds are needed. It allows rapid and simple quantitation of cannabinoids with a final analysis time of only 5 min without the need for a pre-purification step.
Hazekamp
Arno
A
Division of Pharmacognosy, Institute of Biology, Leiden University, Einsteinweb 55, 2300 RA Leiden, The Netherlands. ahazekamp@rocketmail.com
Choi
Young Hae
YH
Verpoorte
Robert
R
D063385:Cannabinoid Receptor Modulators / Q000032:analysis* / Q000737:chemistry; D002188:Cannabis / Q000737:chemistry*; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry
D063385:Cannabinoid Receptor Modulators; D010936:Plant Extracts; D006859:Hydrogen