[Accelerated hydrophobic chromatography of plant extracts: a promising tool for study of biologically active polyphenols].
A simple and efficient technique for fast isolation and purification of a total polyphenol fraction from dry herbal plant extract ("Phytoprost" preparation) has been developed. The method is based on sequential cascade of 1.0-5.0 kDa ultrafiltration and the following subsequent column chromatography of resultant oligo/polymeric compounds on Phenyl-Sepharose 4B-CL. This novel procedure allows to obtain a highly purified (not more than 0.5-0.6% of aromatic and heterocyclic impurities) fraction of plant polyphenols in amounts required for pharmacological/biochemical screening (0.85 +/- 0.06 A280/g dry extract) within 8-9 hours.
Markarian
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AA
I.M. Sechenov Moscow Medical Academy, Moscow, Russia 119881, Moscow, M. Trubetskaya, 8. markaria@rol.ru
Dargaeva
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TD
Aliautdin
R N
RN
Burkov
V L
VL
D002850:Chromatography, Gel / Q000379:methods; D005419:Flavonoids / Q000032:analysis*; D057927:Hydrophobic and Hydrophilic Interactions; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D059808:Polyphenols; D014462:Ultrafiltration
D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols