Generation and characterization of ras lipoproteins based on chemical coupling.
Chemically synthesized truncated Ras proteins are coupled to C-terminal Ras peptides via a maleimidocaproyl linker. The resulting product is isolated by extraction with Triton X-114. The biological activity of these oncogenic Ras lipoproteins can be determined in a cell-based differentiation assay by microinjection into PC12 cells.
Wagner
Melanie
M
Max-Planck-Institut für Molekulare Physiologie, Dortmund, Germany.
Kuhlmann
Jürgen
J
D000818:Animals; D001681:Biological Assay / Q000379:methods; D002454:Cell Differentiation; D008074:Lipoproteins / Q000138:chemical synthesis* / Q000737:chemistry; D008845:Microinjections; D016716:PC12 Cells; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis; D018631:ras Proteins / Q000096:biosynthesis / Q000737:chemistry*
D008074:Lipoproteins; D011994:Recombinant Proteins; D018631:ras Proteins