Modeling of monolith-supported affinity chromatography.
Ceramic monoliths have been used successfully as active support for affinity chromatography (1). A mathematical model was developed to simulate the adsorption-elution experimental behavior of asparaginase in an agarose-coated monolith support. The computer-based model allows precise determination of experimental parameters. Because of the simple geometry of ceramic monoliths used as support, the mathematical model can be used to design adsorption/elution cycles for the large throughput separation of biomolecules.
Montes Sanchez
Francisco J
FJ
Department of Chemical and Materials Engineering, University of Alabama in Huntsville, Huntsville, Alabama 35899, USA.
Martin del Valle
Eva
E
Galan Serrano
Miguel A
MA
Cerro
Ramon L
RL
D000327:Adsorption; D001215:Asparaginase / Q000737:chemistry* / Q000302:isolation & purification*; D002516:Ceramics; D002846:Chromatography, Affinity / Q000379:methods*; D002847:Chromatography, Agarose / Q000379:methods*; D045424:Complex Mixtures / Q000737:chemistry / Q000302:isolation & purification; D003198:Computer Simulation; D007700:Kinetics; D044085:Microfluidics / Q000379:methods*; D008956:Models, Chemical; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification; D014462:Ultrafiltration / Q000379:methods*
D045424:Complex Mixtures; D011506:Proteins; D001215:Asparaginase