Enhanced expression of an antibody-targeted plasminogen activator in Escherichia coli by fusion to decorsin.
To improve the thrombolytic specificity of plasminogen activators, an antibody-targeted plasminogen activator was constructed consisting of a single-chain variable fragment of a monoclonal antibody SZ-51 raised specifically against human P-selectins on activated platelets and a low molecular weight single-chain urokinase. After fusion to the 3' end of the gene coding for decorsin, originally isolated from the leech Macrobdella decora, expression of the antibody-targeted plasminogen activator gene in E. coli strain Rosetta (DE3) pLysS was greatly enhanced.
Yu
Ming
M
National Key Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, PR China.
Yu
Meimin
M
Ru
Binggen
B
D000818:Animals; D000845:Annelida; D000911:Antibodies, Monoclonal / Q000737:chemistry; D001483:Base Sequence; D001792:Blood Platelets / Q000378:metabolism; D015153:Blotting, Western; D015815:Cell Adhesion Molecules; D002614:Chelating Agents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000378:metabolism*; D005342:Fibrinolysis; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D019007:P-Selectin / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D010960:Plasminogen Activators / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism; D015539:Platelet Activation; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D014568:Urokinase-Type Plasminogen Activator / Q000737:chemistry; D015032:Zinc / Q000737:chemistry
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D002614:Chelating Agents; D019007:P-Selectin; D011506:Proteins; C064478:decorsin; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator; D015032:Zinc