Adenoviral vector-mediated rescue of the OMP-null behavioral phenotype: enhancement of odorant threshold sensitivity.
Mice from which the olfactory marker protein (OMP) gene has been deleted demonstrate a number of neurophysiologic and behavioral defects that suggest OMP is an important component in olfactory signal transduction and is critically involved in odor processing. Recently, the potential pleiotropic effects of gene deletion were addressed by adenoviral vector-mediated rescue of the neurophysiologic defects, in vivo. As a complement to this study, the authors used a recombinant adenoviral vector to transiently introduce OMP into olfactory sensory neurons of adult OMP-null mice and, using psychophysical methods, demonstrated the resulting reacquisition of behavioral function subsequent to gene replacement. The rescue of the OMP-null behavioral phenotype further supports the hypothesis that OMP is an important component in olfactory signal amplification and/or transduction processing.
Youngentob
Steven L
SL
Department of Neuroscience and Physiology, State University of New York Upstate Medical University, 750 East Adams Street, Syracuse, NY, USA. youngens@mail.upstate.edu
Pyrski
Martina M
MM
Margolis
Frank L
FL
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D003216:Conditioning, Operant; D004193:Discrimination Learning; D004305:Dose-Response Relationship, Drug; D018304:Esthesioneuroblastoma, Olfactory / Q000378:metabolism / Q000821:virology; D005822:Genetic Vectors / Q000378:metabolism / Q000494:pharmacology; D049452:Green Fluorescent Proteins; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007239:Infections; D008164:Luminescent Proteins / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology*; D009812:Odorants; D051842:Olfactory Marker Protein; D018034:Olfactory Receptor Neurons / Q000378:metabolism; D010641:Phenotype; D011897:Random Allocation; D012684:Sensory Thresholds / Q000187:drug effects / Q000502:physiology*; D014162:Transfection
D008164:Luminescent Proteins; D009419:Nerve Tissue Proteins; C497597:OMP protein, human; D051842:Olfactory Marker Protein; C497598:Omp protein, mouse; D049452:Green Fluorescent Proteins