Differential regulation of ciliary neurotrophic factor and its receptor in the rat hippocampus in response to kainic acid-induced excitotoxicity.
We analyzed the changes in expression of ciliary neurotrophic factor (CNTF) and its receptor, ligand-binding subunit a (CNTFRa), in the hippocampus following intraperitoneal administration of a convulsant dose of kainic acid (KA). Immunohistochemistry and immunoblotting showed that CNTF levels rose dramatically between day 1 and day 10, and that the CNTF was located in reactive astrocytes. In contrast, upregulation of CNTFRalpha mRNA, occurred in neurons as well as astrocytes. A rapid, and short-lived (3 h-2 d) increase in CNTFRalpha was also observed in the more resistant granule cells and CA2 pyramidal neurons. The increase in astrocytes was detected by day 1 and was sustained for more than 5 d. These results show that CNTF and CNTFRalpha are differentially regulated in hippocampal neurons and reactive astrocytes following KA injection, indicating that these proteins may be involved in the regulation of astrocyte and neuronal degenerative responses.
Choi
Jeong-Sun
JS
Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea.
Kim
Seong Yun
SY
Park
Hyun-Jung
HJ
Cha
Jung-Ho
JH
Choi
Yun-Sik
YS
Chung
Jin-Woong
JW
Chun
Myung-Hoon
MH
Lee
Mun-Yong
MY
D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D020934:Ciliary Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D003292:Convulsants / Q000494:pharmacology; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology / Q000633:toxicity*; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007608:Kainic Acid / Q000494:pharmacology / Q000633:toxicity*; D008297:Male; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D009498:Neurotoxins; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020801:Receptor, Ciliary Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
D020934:Ciliary Neurotrophic Factor; D003292:Convulsants; D018690:Excitatory Amino Acid Agonists; D009498:Neurotoxins; D021122:Protein Subunits; D020801:Receptor, Ciliary Neurotrophic Factor; D007608:Kainic Acid