[Expression and biological activities of TRAIL fusion protein gene].
OBJECTIVE
To obtain and express the full-length of TRAIL gene in prokaryocytes.


METHODS
The full-length of TRAIL gene was amplified from peripheral blood cells(PBCs)by RT-PCR and then cloned into the expression vector pGEX-2T. The TRAIL-GST was expressed in E.coli.


RESULTS
Sequence analysis indicated that the sequence of TRAIL gene was identical with that in the GenBank. The TRAIL-GST was expressed in E.coli. Expressed product could inhibit the proliferation of KB and Hela cells and finally lead to their apoptosis.


CONCLUSIONS
The TRAIL-GST with biological activity has been expressed in the prokaryotic system.
Li
Jing
J
Institute of Beijing Infectious Diseases, Beijing 100039, China.
Zheng
Yu
Y
Chen
Hao
H
Du
Li
L
Shu
Cui-li
CL
Cheng
Yun
Y
D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins; D005434:Flow Cytometry; D005982:Glutathione Transferase / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D007624:KB Cells; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D053221:TNF-Related Apoptosis-Inducing Ligand; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000502:physiology
D051017:Apoptosis Regulatory Proteins; D008562:Membrane Glycoproteins; D011993:Recombinant Fusion Proteins; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; D014409:Tumor Necrosis Factor-alpha; D005982:Glutathione Transferase