title: Genotype and allele frequency of PAI-1 promoter polymorphism in healthy subjects from the west of Mexico. Association with biochemical and hematological parameters.
abstract: We investigated the genotype and allelic frequency of the -675 bp insertion/deletion polymorphism at the PAI-1 gene promoter, in healthy Mexican subjects. It was compared to the lipid profile and hematological parameters, and to other healthy worldwide populations. A Mexican population sample of 110 individuals was studied. Demographic data and clinical characteristics of the subjects were registered. Fasting lipid profile, serum glucose, fibrinogen, hematological parameters and leukocyte genomic DNA isolation from peripheral blood were performed in all the participants. Screening of the PAI-1 genotype was done by PCR and restriction analysis. Genotype 4G/4G, 4G/5G, 5G/5G frequency in Mexican healthy subjects was: 14.55%, 39.09%, 46.36%, respectively, whereas the allelic frequency for 5G allele was 65.9%. A significant lesser frequency for 4G allele and related genotypes (4G/4G and 4G/5G) was established in healthy subjects from Mexico, respect to all the compared populations. A particular genotype and allelic frequency of this PAI-1 polymorphism was established in Mexico. The clinical parameters were not associated according to each genotype of PAI-1.
authors.affiliation: Laboratorio de Inmunología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada Street No. 950, P.O. Box 2-1091, Guadalajara, Jalisco, Mexico, C.P. 44281, Mexico.
authors.forename: Sandra
authors.initials: S
authors.lastname: Ruiz-Quezada
authors.forename: Mónica
authors.initials: M
authors.lastname: Vázquez-Del Mercado
authors.forename: Isela
authors.initials: I
authors.lastname: Parra-Rojas
authors.forename: Héctor
authors.initials: H
authors.lastname: Rangel-Villalobos
authors.forename: Carlos
authors.initials: C
authors.lastname: Best-Aguilera
authors.forename: Laura Verónica
authors.initials: LV
authors.lastname: Sánchez-Orozco
authors.forename: José Francisco
authors.initials: JF
authors.lastname: Muñoz-Valle
mesh_terms: D000328:Adult; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006403:Hematologic Tests; D006801:Humans; D008055:Lipids / Q000097:blood*; D008297:Male; D008800:Mexico; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics*; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D012016:Reference Values
chemical_list: D008055:Lipids; D017395:Plasminogen Activator Inhibitor 1