Regulation of apical surface fluid and protein secretion in human airway epithelial cell line Calu-3.
We evaluated the relationship between apical surface fluid (ASF) and protein secretion in Calu-3 cells grown at an air-liquid interface. Calu-3 monolayers responded to forskolin, a cystic fibrosis transmembrane regulator (CFTR) channel agonist, by secreting a significant amount of ASF. Such a response from Calu-3 monolayers was not observed with CFTR channel blockers glybenclamide and DPC. Other ion channel mediators, PGF-2alpha, PMA, DNDS, and DIDS, had no effect on Calu-3 ASF secretion. Forskolin decreased Calu-3 protein secretion and glybenclamide increased protein secretion. Similarly, forskolin decreased Calu-3 lysozyme secretion, whereas glybenclamide and DPC increased lysozyme secretion. We observed significant changes in Calu-3 fluid and protein secretions with ion channel mediators known to alter CFTR activity. Our results demonstrate a functional link between fluid and protein secretions in Calu-3 apical surface and suggested a possible involvement of CFTR in these processes.
Ramesh Babu
Polani B
PB
Nemours Research Lung Center, Alfred I. duPont Hospital for Children, Wilmington, DE, USA. rbabu@nemours.org
Chidekel
Aaron
A
Utidjian
Levon
L
Shaffer
Thomas H
TH
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology; D001826:Body Fluids / Q000378:metabolism*; D002460:Cell Line; D016764:Cell Polarity; D005576:Colforsin / Q000494:pharmacology; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000378:metabolism*; D015237:Dinoprost / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005905:Glyburide / Q000494:pharmacology; D006801:Humans; D009113:Muramidase / Q000378:metabolism; D010120:Oxytocics / Q000494:pharmacology; D020545:Respiratory Mucosa / Q000166:cytology*; D013267:Stilbenes / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000889:Anti-Arrhythmia Agents; C505032:CFTR protein, human; D010120:Oxytocics; D013267:Stilbenes; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D005576:Colforsin; C022306:4,4'-dinitro-2,2'-stilbenedisulfonic acid; D015237:Dinoprost; D009113:Muramidase; D013755:Tetradecanoylphorbol Acetate; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D005905:Glyburide