A comparative study of the subcellular distribution of native and deglycosylated gelonin in rat liver and kidney.
Intravenous injection of gelonin and deglycosylated gelonin led to rapid clearance from the blood. Both molecules distributed similarly in liver and kidney suggesting that they followed the same pathway. Deglycosylation reduced the uptake by a third in liver, but did not affect uptake by kidney. Studies with Triton WR1339 showed a classical lysosomal pathway for both molecules. The deglycosylated molecule was degraded to a greater extent than native gelonin as seen by the presence of acid soluble radioactivity. Cell separation showed that while endothelial cells mainly took up native gelonin, Kupffer cells took up the deglycosylated molecule.
Colaço
Melwin
M
Department of Biochemistry, The Institute of Science, 15 Madam Cama Road, Mumbai 400 032, India.
Misquith
Sandra
S
Bapat
M M
MM
Wattiaux-De Coninck
Simone
S
Wattiaux
Robert
R
D000818:Animals; D002498:Centrifugation; D006031:Glycosylation; D007457:Iodine Radioisotopes / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D011500:Protein Synthesis Inhibitors / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D054789:Ribosome Inactivating Proteins, Type 1; D013347:Subcellular Fractions / Q000737:chemistry / Q000378:metabolism*
D007457:Iodine Radioisotopes; D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D054789:Ribosome Inactivating Proteins, Type 1; C025674:GEL protein, Gelonium multiflorum