title: Determination of 2-(diethylamino)-N-(2,6-dimethylphenyl) acetamide in a gel pharmaceutical formulation by high-performance liquid chromatography.
abstract: An isocratic reversed-phase high performance liquid chromatographic method is developed and validated for the determination of 2-(diethylamino)-N-(2,6-dimethylphenyl) acetamide (0.3%, w/w) in a gel formulation. The chromatographic separation is achieved with potassium phosphate buffer (pH 7.0)-acetonitrile (47:53, v/v) as mobile phase, a C(18) column, and UV detection at 254 nm. The calibration curve is linear (r(2) = 1.000) from 20-140% of the analytical concentration of 1.4 microg/mL. The mean percent relative standard deviation values for intra- and interday precision studies are < 1%. The recovery ranges 99.95-100.23% from a gel formulation. The method is specific and successfully routinely used in quality control for the analysis of bulk gel samples and final product release.
authors.affiliation: Fleet Laboratories, Watford, Hertfordshire WD18 7JJ, U.K. ghulam.shabir@abbott.com
authors.forename: Ghulam A
authors.initials: GA
authors.lastname: Shabir
mesh_terms: D000081:Acetamides / Q000032:analysis*; D000779:Anesthetics, Local / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008012:Lidocaine; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000081:Acetamides; D000779:Anesthetics, Local; D004364:Pharmaceutical Preparations; D008012:Lidocaine