GABAA receptor alpha1 and alpha6 subunits mediate cell surface anchoring in cultured cells.
The clustering and immobility of gamma-aminobutyric acid type A receptors (GABAARs) at discrete and functionally significant domains on the nerve cell surface is an important determinant in the integration of synaptic inputs. To investigate the role that different GABAAR alpha subunit isoforms play in determining receptor mobility, alphaxbeta3gamma2s subunits (where x = subunit isoforms 1-6) were co-transfected into COS 7 and human embryonic kidney (HEK) 293 cells and the surface mobility of these recombinant complexes was measured by fluorescence photobleach recovery (FPR). In addition, the lateral mobility of endogenous GABAARs in cerebellar granule (CG) cells was measured. We show that the alpha1 and alpha6 subunits immobilize recombinant GABAAR in transfected cells. This is consistent with the immobility of native receptors in CG cells, which express alpha1 and alpha6.
Peran
Macarena
M
Departamento de Bioquímica, Facultad de Medicina, Universidad de Málaga, Campus de Teatinos, Málaga 29080, Spain.
Hooper
Helen
H
Rayner
Sharon L
SL
Stephenson
F Anne
FA
Salas
Ramiro
R
D000818:Animals; D001896:Boron Compounds; D019556:COS Cells; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D005456:Fluorescent Dyes; D006801:Humans; D011956:Receptors, Cell Surface / Q000235:genetics / Q000502:physiology*; D011963:Receptors, GABA-A / Q000235:genetics / Q000502:physiology*; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection
D001896:Boron Compounds; D018076:DNA, Complementary; D005456:Fluorescent Dyes; C546240:GABRA1 protein, human; C546171:GABRA6 protein, human; D011956:Receptors, Cell Surface; D011963:Receptors, GABA-A; D011994:Recombinant Proteins; C090668:Ro 1986