title: Identification of hibernating myocardium by acoustic microscopy.
abstract: Hibernating myocardium is viable myocardium that recovers after revascularization. The observation of loss of contractile proteins (myofibrils) and accumulation of glycogen in hibernating cardiomyocytes provide the basis for diagnosing hibernating myocardium. In this pilot study, acoustic microscopy was used to identify the cellular structure of normal vs. hibernating myocardium. Sections cut at 5-microm of archival paraffin blocks on glass slides were used for this study. Acoustic microscopy of normal cardiomyocytes showed intracellular linear echoes suggestive of myofibrils, and cardiomyocytes of hibernating myocardium revealed absence of myofibrils and dense intracellular echoes that corresponded to glycogen accumulation on optical microscopy. This modality of visualization allows a definitive diagnosis of hibernating myocardium.
authors.affiliation: Division of Cardiology, LAC+USC Medical Center, University of Southern California School of Medicine, Los Angeles, CA, USA.
authors.forename: Daniel Wing Chong
authors.initials: DW
authors.lastname: Ng
authors.forename: Shamachary
authors.initials: S
authors.lastname: Sathish
authors.forename: Aameera
authors.initials: A
authors.lastname: Khan
authors.forename: Parakrama
authors.initials: P
authors.lastname: Chandrasoma
authors.forename: William
authors.initials: W
authors.lastname: Wijns
authors.forename: P A N
authors.initials: PA
authors.lastname: Chandraratna
mesh_terms: D006003:Glycogen / Q000032:analysis; D006801:Humans; D033401:Microscopy, Acoustic / Q000379:methods*; D017682:Myocardial Stunning / Q000000981:diagnostic imaging*; D032383:Myocytes, Cardiac / Q000000981:diagnostic imaging / Q000502:physiology; D009210:Myofibrils / Q000000981:diagnostic imaging; D010865:Pilot Projects; D014617:Vacuoles / Q000000981:diagnostic imaging
chemical_list: D006003:Glycogen