[Typing for HLA-DR DNA by polymerase chain reaction with sequence-specific primers].
OBJECTIVE
To type the HLA-DR DNA for renal transplantation by PCR with sequence specific primers (PCR-SSP).


METHODS
According to nucleotide sequences of HLA-DR, 16 pairs of specific primers and a pair of positive control primers were designed and synthesized for PCR-SSP. Then HLA-DR sites of 52 donors and recipients for renal transplantation were typed by the PCR-SSP.


RESULTS
All the samples were successfully typed by PCR-SSP with the synthesized primers. The results were available within 3 hours after sampling and the accuracy and reproducibility were 100%.


CONCLUSIONS
Genotyping for HLA-DR sites by PCR-SSP with primers reported herein was a simple and accurate technique suitable for clinical application.
Wu
Guo-jun
GJ
Department of Urology Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. wuguojun@fmmu.edu.cn
Wang
He
H
Yu
Lei
L
Yuan
Jian-lin
JL
Zhang
Bo
B
Guo
Wei
W
Wang
Dong
D
Li
Xin
X
D000293:Adolescent; D000328:Adult; D017931:DNA Primers; D005260:Female; D005838:Genotype; D006684:HLA-DR Antigens / Q000235:genetics*; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D006684:HLA-DR Antigens