PKA-phosphorylation of PDE4D3 facilitates recruitment of the mAKAP signalling complex.
mAKAP (muscle-selective A-kinase-anchoring protein) co-ordinates a cAMP-sensitive negative-feedback loop comprising PKA (cAMP-dependent protein kinase) and the cAMP-selective PDE4D3 (phosphodiesterase 4D3). In vitro and cellular experiments demonstrate that PKA-phosphorylation of PDE4D3 on Ser-13 increases the affinity of PDE4D3 for mAKAP. Our data suggest that activation of mAKAP-anchored PKA enhances the recruitment of PDE4D3, allowing for quicker signal termination.
Carlisle Michel
Jennifer J
JJ
Howard Hughes Medical Institute and Vollum Institute, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239-3098, USA.
Dodge
Kimberly L
KL
Wong
Wei
W
Mayer
Nicole C
NC
Langeberg
Lorene K
LK
Scott
John D
JD
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D048868:Adaptor Proteins, Signal Transducing / Q000502:physiology*; D001665:Binding Sites; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4; D004789:Enzyme Activation / Q000502:physiology; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000196:embryology / Q000201:enzymology; D018716:Molecular Mimicry / Q000502:physiology; D010446:Peptide Fragments / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D010766:Phosphorylation; D011485:Protein Binding / Q000502:physiology; D025941:Protein Interaction Mapping; D012694:Serine / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D010446:Peptide Fragments; D010455:Peptides; D012694:Serine; D017868:Cyclic AMP-Dependent Protein Kinases; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4