title: Intracellular IFN-gamma expression by CD3+/CD8+ cell subset in B-CLL patients correlates with stage of the disease.
abstract: Changes in the cytokine network may be responsible for malignant cell accumulation in B-cell chronic lymphocytic leukaemia (B-CLL). Among different cytokines of question interferon gamma (IFN-gamma) is indicated to prevent malignant cells from entering apoptosis. The aim of the study was to determine IFN-gamma production capacity of T-cell subsets and B lymphocytes in B-CLL patients in comparison with healthy individuals and during disease progression. Forty patients with newly diagnosed, untreated B-CLL and 20 healthy individuals were studied. The two- and three-colour flow cytometry techniques were used to detect intracellular cytokine expression. We detected statistically significantly higher percentage of both CD3+/CD4+/IFN-gamma+ and CD3+/CD8+/IFN-gamma+ in patients than in controls (P < 0.001 in both cases). Moreover the percentage of CD3+/CD8+/IFN-gamma+ cells correlated with stage of the disease (R = 0.39, P = 0.01) and parameters of disease progression like lymphocyte count and total tumour mass score (R = 0.33, P = 0.03 and R = 0.31, P = 0.04, respectively). By contrast, the percentage of CD19+/IFN-gamma+ cells in B-CLL group was lower than in controls (P < 0.01). These findings indicate that T-cell populations rather than malignant B cells are the source of IFN-gamma in B-CLL patients. The subset of CD3+/CD8+ cells expressing IFN-gamma seems to play a special role in the disease progression and more precise investigation should elucidate its role as a prognostic marker in B-CLL and a target for therapeutic strategies.
authors.affiliation: Department of Haematology, University School of Medicine in Lublin, Jaczewskiego, Lublin, Poland. mpodhor@asklepios.am.lublin.pl
authors.forename: Monika
authors.initials: M
authors.lastname: Podhorecka
authors.forename: Anna
authors.initials: A
authors.lastname: Dmoszynska
authors.forename: Jacek
authors.initials: J
authors.lastname: Rolinski
mesh_terms: D018941:Antigens, CD19 / Q000032:analysis; D017252:CD3 Complex / Q000032:analysis*; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry*; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism* / Q000473:pathology*; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000737:chemistry* / Q000276:immunology; D009367:Neoplasm Staging
chemical_list: D018941:Antigens, CD19; D017252:CD3 Complex; D007371:Interferon-gamma