The proliferation associated nuclear element (PANE1) is conserved between mammals and fish and preferentially expressed in activated lymphoid cells.
The proliferation associated nuclear element (PANE1) had been identified in a screen for genes activated in mouse mammary epithelium transformed by stabilized beta-catenin. We have now cloned the human and zebrafish orthologs, analyzed their expression and expressed them ectopically in tissue culture cell lines. PANE1 consists of 180 amino acids and displays 38% conservation between man and zebrafish. Expression of the human PANE1 gene was detected preferentially in immune cells including leukemias and lymphomas, tumor tissues and tumor derived cell lines. In B- and T-cells PANE1 RNA was only detected after the respective cell types were activated either in vivo or in vitro.
Bierie
Brian
B
Laboratory of Genetics and Physiology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 8, Room 101, Bethesda, MD 20892-0822, USA.
Edwin
Micah
M
Melenhorst
J Joseph
JJ
Hennighausen
Lothar
L
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D006801:Humans; D008213:Lymphocyte Activation / Q000235:genetics / Q000502:physiology*; D051379:Mice; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000378:metabolism*; D015027:Zebrafish / Q000235:genetics; D029961:Zebrafish Proteins / Q000235:genetics*
C495719:CENPM protein, human; D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; C495721:PANE1 protein, zebrafish; D029961:Zebrafish Proteins; C495720:proliferation associated nuclear element 1 protein, mouse