Nail and bone surface as biomarkers for acute fluoride exposure in rats.
When acute exposure to fluoride is thought to be the cause of death, confirmation often depends on the analysis of some body fluid or tissue. The aim of this study was to evaluate the use of nails and the periosteal surface of bone as indicators of acute exposure to fluoride. Six groups of rats were given a single oral dose of fluoride (50 mg/kg body weight), while the control group was given deionized water. The rats were killed at 2, 4, 8, 16, 24, and 48 h after fluoride administration. Plasma and nails (the proximal halves) were collected and analyzed for fluoride with an ion-specific electrode after hexamethyldisiloxane-facilitated diffusion. A circular area of the femur (4.52 mm(2)) was etched with 0.5M HCl for 15 s, and, after the addition of a buffer, the solution was analyzed with an ion-specific electrode. Peak plasma concentration occurred at 2 h, followed by progressively declining concentrations. Peak nail fluoride concentrations occurred at 8 h. The mean nail concentrations at 8, 16, and 24 h were significantly higher than that of the control group. Bone surface concentrations were significantly higher than that of the control group at 4 h and thereafter. Thus, the proximal portion of nails and bone surface are suitable biomarkers for acute fluoride exposure in rats.
Buzalaf
Marília Afonso Rabelo
MA
Departamento de Ciências Biológicas, Faculdade de Odontologia de Bauru, Universidade de São Paulo, Alameda Octávio Pinheiro Brisolla 9-75, 17012-901 Bauru, SP, Brazil.
Caroselli
Elide Escolástico
EE
Cardoso de Oliveira
Rodrigo
R
Granjeiro
José Mauro
JM
Whitford
Gary Milton
GM
D000818:Animals; D015415:Biomarkers; D005269:Femur / Q000737:chemistry* / Q000378:metabolism; D005458:Fluoride Poisoning / Q000378:metabolism*; D005459:Fluorides / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D006724:Hoof and Claw / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014018:Tissue Distribution
D015415:Biomarkers; D005459:Fluorides