Association between surfactant protein B + 1580 polymorphism and the risk of respiratory failure in adults with community-acquired pneumonia.
OBJECTIVE
Pulmonary surfactant protein (SP)-B plays a vital role in the formation and function of surfactant in the lung. A genetic polymorphism (SP-B + 1580) is postulated to result in diminished activity of SP-B. The objective was to determine whether the SP-B + 1580 CC genotype is associated with an increased risk of respiratory failure and ARDS in adults with community-acquired pneumonia.


METHODS
Prospective cohort of adults diagnosed with community-acquired pneumonia.


METHODS
Hospital system.


METHODS
We enrolled 402 adults > or = 18 yrs of age with community-acquired pneumonia; 158 were white, 243 were African American, and one was Asian.


METHODS
Genotypic analysis was performed on DNA isolated from whole blood using polymerase chain reaction amplification and DdeI restriction enzyme digestion.


RESULTS
We recorded the requirement for mechanical ventilation, the presence of acute respiratory distress syndrome (ARDS) or septic shock, and mortality. Sixty-three patients required mechanical ventilation, 12 patients developed ARDS, and 35 patients developed septic shock. Genotypic frequencies at the SP-B + 1580 site were T/T 183 of 402 (0.45), T/C 160 of 402 (0.40), and C/C 59 of 402 (0.15). Of the 59 patients who were C/C at the SP-B + 1580 site, 21 (0.356) required mechanical ventilation, compared with 26 of 160 patients (0.163) who were T/C and 16 of 183 (0.087) patients who were T/T (p < .001). ARDS developed in five of 59 (0.085) patients with the C/C genotype, compared with six of 160 (.038) patients with T/C and one of 183 patients with T/T (0.005, p < .009). Septic shock occurred in 12 of 59 (0.203) patients with the C/C genotype, compared with 13 of 160 (0.081) patients with T/C and ten of 183 (0.055) patients with T/T (p < .001). Mortality rate was not different between the three genotypes.


CONCLUSIONS
Carriage of the C allele at the SP-B + 1580 site is associated with ARDS, septic shock, and the need for mechanical ventilation in adults with community-acquired pneumonia.
Quasney
Michael W
MW
Division of Critical Care, Department of Pediatrics, University of Tennessee, Memphis, Children's Foundation Research Center of Memphis, Le Bonheur Children's Medical Center, 50 N. Dunlap, Memphis, TN 38103, USA. mquasney@utmem.edu
Waterer
Grant W
GW
Dahmer
Mary K
MK
Kron
Grace K
GK
Zhang
Qing
Q
Kessler
Lori A
LA
Wunderink
Richard G
RG
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D002423:Cause of Death; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics; D017714:Community-Acquired Infections / Q000150:complications*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D017052:Hospital Mortality; D006801:Humans; D008297:Male; D008875:Middle Aged; D011014:Pneumonia / Q000150:complications*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D011446:Prospective Studies; D037701:Pulmonary Surfactant-Associated Protein B / Q000235:genetics* / Q000502:physiology; D012121:Respiration, Artificial / Q000706:statistics & numerical data; D012128:Respiratory Distress Syndrome / Q000209:etiology / Q000401:mortality / Q000628:therapy; D012131:Respiratory Insufficiency / Q000209:etiology* / Q000401:mortality / Q000628:therapy; D015183:Restriction Mapping; D012307:Risk Factors; D012772:Shock, Septic / Q000209:etiology / Q000401:mortality / Q000628:therapy; D013714:Tennessee / Q000453:epidemiology
D037701:Pulmonary Surfactant-Associated Protein B