title: [Effect of ATRA and DNR on the expression and secretion of VEGF in leukemic cells].
abstract: OBJECTIVE
  To investigate the expression of VEGF mRNA and secretion of VEGF protein in NB4 and HL-60 cells affected by all-trans retinoic acid (ATRA) and daunorubincin (DNR) respectively.
  
  
  METHODS
  Semi-quantitative RT-PCR and ELISA were used to study the expression of VEGF mRNA and secretion of VEGF protein in NB4 and HL-60 cell lines treated by ATRA and DNR respectively.
  
  
  RESULTS
  VEGF was expressed in both NB4 and HL-60 cells. The expression of VEGF mRNA and secretion of VEGF protein could be down-regulated by ATRA and DNR respectively in a time and dose dependent manner.
  
  
  CONCLUSIONS
  Besides inducing apoptosis and restraining proliferation of leukemic cells, ATRA and DNR exerted their anti-leukemia effects by reducing angiogenesis via reduction of angiogenic reaction stimulating signals.
authors.affiliation: Department of Leukemia Research, Shanghai Institute of Hematology, Shanghai 200001, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Fang-Yuan
authors.initials: FY
authors.lastname: Chen
authors.forename: Chun-Hong
authors.initials: CH
authors.lastname: Gu
authors.forename: Jie-Ying
authors.initials: JY
authors.lastname: Han
authors.forename: Hua
authors.initials: H
authors.lastname: Zhong
authors.forename: Ji-Hua
authors.initials: JH
authors.lastname: Zhong
authors.forename: Ye
authors.initials: Y
authors.lastname: Teng
authors.forename: Ren-Rong
authors.initials: RR
authors.lastname: Ouyang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003630:Daunorubicin / Q000494:pharmacology*; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D014212:Tretinoin / Q000494:pharmacology*; D042442:Vascular Endothelial Growth Factors / Q000235:genetics* / Q000378:metabolism*
chemical_list: D042442:Vascular Endothelial Growth Factors; D014212:Tretinoin; D003630:Daunorubicin