[Effects of tea polyphenols and tea pigments on telomerase activity of HepG2 cells].
OBJECTIVE
This study is to investigate the effect of tea polyphenols and tea pigments on telomerase activity of human liver cancer cell line, HepG2 cells.


METHODS
TRAP-PCR-ELISA was applied to investigate the telomerase activity.


RESULTS
Telomerase was positive in tea polyphenols treated groups, tea pigments treated groups and blank control group. Telomerase activities (A(450 approximately 690) values) were 1.56 and 1.46 in 50 mg/L and 100 mg/L tea polyphenols-treated groups, 1.55 and 1.49 in 50 mg/L and 100 mg/L tea pigments-treated groups, respectively. The results showed that telomerase activity was significantly inhibited by tea polyphenols and tea pigments treatment as compared with the blank control group (A(450 approximately 690) = 2.11).


CONCLUSIONS
Tea polyphenols and tea pigments could significantly inhibit telomerase activity of HepG2 cells, and telomerase activity may be a useful biomarker for cancer chemoprevention.
Jia
Xu-dong
XD
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Han
Chi
C
Chen
Jun-shi
JS
D014408:Biomarkers, Tumor / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000473:pathology; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology / Q000473:pathology; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010860:Pigments, Biological / Q000302:isolation & purification / Q000494:pharmacology*; D059808:Polyphenols; D013662:Tea / Q000737:chemistry*; D019098:Telomerase / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D005419:Flavonoids; D010636:Phenols; D010860:Pigments, Biological; D059808:Polyphenols; D013662:Tea; D019098:Telomerase