title: PER-1 type beta-lactamase production in Acinetobacter baumannii is related to cell adhesion.
abstract: BACKGROUND
  Recently, the dissemination of ESBL (PER-1) among Acinetobacter isolates was reported in Turkish hospitals. We investigated the presence and the association of various virulence determinants in 20 Acinetobacter baumannii isolates, of which 13 were blaPER-1 positive.
  
  
  METHODS
  Virulence tests were slime and hemolysin production, gelatinase and protease activity, biofilm formation, and Caco2 cell adhesion. RAPD analysis was also performed with ERIC primers.
  
  
  RESULTS
  None of the strains was positive for slime or hemolysin production and gelatinase or protease activity. A total of 16 strains, five of which were PER-1 gene negative, formed a biofilm on polymer surfaces. There was no relation between the presence of the PER gene and biofilm formation. On the other hand, nine strains (all PER-1 gene positive) were positive in adhesion experiments to Caco2 cell lines. All PER-negative strains were negative for cell adhesion.
  
  
  CONCLUSIONS
  This study indicates the existence of a relation between PER-1 gene and cell adhesion in Acinetobacter strains.
authors.affiliation: Dipartimento di Scienze biomediche, Sezione di Microbiologia sperimentale e clinica, University of Sassari, Viale San Pietro 43b, 07100 Sassari, Italy. sechila@uniss.it
authors.forename: Leonardo A
authors.initials: LA
authors.lastname: Sechi
authors.forename: Aynur
authors.initials: A
authors.lastname: Karadenizli
authors.forename: Antonella
authors.initials: A
authors.lastname: Deriu
authors.forename: Stefania
authors.initials: S
authors.lastname: Zanetti
authors.forename: Fetiye
authors.initials: F
authors.lastname: Kolayli
authors.forename: Erdener
authors.initials: E
authors.lastname: Balikci
authors.forename: Haluk
authors.initials: H
authors.lastname: Vahaboglu
mesh_terms: D040981:Acinetobacter baumannii / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity*; D001422:Bacterial Adhesion; D018441:Biofilms; D018938:Caco-2 Cells; D016172:DNA Fingerprinting; D010450:Endopeptidases / Q000378:metabolism; D018093:Gelatinases / Q000378:metabolism; D006460:Hemolysin Proteins / Q000378:metabolism; D006801:Humans; D019105:Random Amplified Polymorphic DNA Technique; D014774:Virulence; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism*
chemical_list: D006460:Hemolysin Proteins; D010450:Endopeptidases; D018093:Gelatinases; D001618:beta-Lactamases