Unexpected observation of ion suppression in a liquid chromatography/atmospheric pressure chemical ionization mass spectrometric bioanalytical method.
Ion suppression is a well-known phenomenon in electrospray ionization (ESI) mass spectrometry. These suppression effects have been shown to adversely affect the accuracy and precision of quantitative bioanalytical methods using ion spray. Such suppression effects have not been as well defined in atmospheric pressure chemical ionization (APCI) and there is some debate whether these effects actually occur in the ionization process using APCI. Here an example is described where clear ion suppression was observed during studies on a model compound and three metabolites using APCI liquid chromatography/tandem mass spectrometry (LC/MS/MS).
Sangster
Tim
T
AstraZeneca, DMPK Alderley, Alderley Park, Macclesfield SK10 4TG, UK. timothy.sangster@astrazeneca.com
Spence
Mike
M
Sinclair
Peta
P
Payne
Richard
R
Smith
Christopher
C
D000605:Amino Alcohols / Q000032:analysis / Q000737:chemistry; D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006900:Hydroxylation; D007477:Ions / Q000737:chemistry*; D008745:Methylation; D051379:Mice; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000605:Amino Alcohols; D007477:Ions