Transgenic mouse line with green-fluorescent protein-labeled Centrin 2 allows visualization of the centrosome in living cells.
The centrosome plays diverse roles throughout the cellular mitotic cycle and in post-mitotic cells. Analysis of centrosome position and dynamics in living murine cells has been limited due to a lack of adequate reporters and currently requires either cell fixation/immunostaining or transfection with centrosome reporters. Here we describe the generation and characterization of a transgenic mouse line that constitutively expresses green fluorescent protein-labeled Centrin-2 (GFP-CETN2). The phenotype of the mouse is indistinguishable from wild-type and it displays a single pair of fluorescent centrioles in cells of every organ and time point examined. This model will be helpful for visualizing the centrosome in multiple experimental conditions.
Higginbotham
Holden
H
Neurogenetics Laboratory, Department of Neurosciences, University of California, San Diego, USA.
Bielas
Stephanie
S
Tanaka
Teruyuki
T
Gleeson
Joseph G
JG
D000818:Animals; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D002467:Cell Nucleus / Q000648:ultrastructure; D002502:Centrioles / Q000648:ultrastructure; D018385:Centrosome / Q000378:metabolism / Q000648:ultrastructure*; D004622:Embryo, Mammalian / Q000166:cytology; D005347:Fibroblasts / Q000648:ultrastructure; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D008856:Microscopy, Fluorescence; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism
D002135:Calcium-Binding Proteins; C424436:Cetn2 protein, mouse; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins