Second-harmonic optical coherence tomography.
Second-harmonic optical coherence tomography, which uses coherence gating of second-order nonlinear optical responses of biological tissues for imaging, is described and demonstrated. Femtosecond laser pulses were used to excite second-harmonic waves from collagen harvested from rat tail tendon and a reference non-linear crystal. Second-harmonic interference fringe signals were detected and used for image construction. Because of the strong dependence of second-harmonic generation on molecular and tissue structures, this technique imparts contrast and resolution enhancement to conventional optical coherence tomography.
Jiang
Yi
Y
Beckman Laser Institute, Department of Biomedical Engineering, University of California, Irvine, Irvine, California 92612, USA.
Tomov
Ivan
I
Wang
Yimin
Y
Chen
Zhongping
Z
D000818:Animals; D001718:Birefringence; D003094:Collagen / Q000648:ultrastructure*; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D051381:Rats; D013623:Tail / Q000166:cytology*; D041623:Tomography, Optical Coherence / Q000379:methods*
D003094:Collagen