Distinct domains of Brn-3a regulate apoptosis and neurite outgrowth in vivo.
The Brn-3a transcription factor is critical for the normal development of the nervous system, promoting both neuronal survival and neurite outgrowth. By manipulating the Brn-3a gene in intact mice, we show that these two functions are separately controlled with an N-terminal domain being essential for neuronal survival, whereas the POU domain is essential for neurite outgrowth. Hence the two naturally occurring forms of Brn-3a, which either contain or lack the N-terminal domain, are likely to play distinct roles in the nervous system.
Faulkes
David J
DJ
Medical Molecular Biology Unit, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK.
Ensor
Elizabeth
E
Le Rouzic
Erwan
E
Latchman
David S
DS
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D016254:Mutagenesis, Insertional; D016501:Neurites / Q000502:physiology*; D009475:Neurons, Afferent / Q000473:pathology / Q000502:physiology / Q000648:ultrastructure; D017434:Protein Structure, Tertiary; D050818:Transcription Factor Brn-3; D050819:Transcription Factor Brn-3A; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D012668:Trigeminal Ganglion / Q000473:pathology
D004268:DNA-Binding Proteins; C494189:Pou4f1 protein, mouse; D050818:Transcription Factor Brn-3; D050819:Transcription Factor Brn-3A; D014157:Transcription Factors