title: [Preparation and characterization of monoclonal antibodies against rat Nogo molecule].
abstract: OBJECTIVE
  To prepare monoclonal antibodies against rat Nogo molecule and study the distribution of Nogo in rat central nervous system (CNS).
  
  
  METHODS
  Murine mAbs were prepared by hybridoma technique. The distribution of Nogo in rat CNS was identified by immunofluorescent histochemical staining.
  
  
  RESULTS
  Three hybridoma cell lines, FMU-Nogo1, FMU-Nogo2 and FMU-Nogo3, secreting mAbs against rat Nogo molecule were obtained. The titers of ascitic mAbs reached to 10(-6) and the Ig subclass of FMU-Nogo1, FMU-Nogo2 and FMU-Nogo3 was IgG2b(kappa), IgG1(kappa) and IgG1(kappa), respectively. Moreover, these mAbs could be used in immunofluoresent histochemical staining. The results showed that rat Nogo molecule could be detected in Purkinje cell layer and granual layer of rat cerebellum and on oligodendrocyte in white matter of rat spinal cord.Furthermore, these mAbs could be used in Western blot for detecting Nogo molecule present in rat spinal cord.
  
  
  CONCLUSIONS
  Three mAbs against rat Nogo molecule were successfully prepared, which can provide a useful tool in research on the structure and functions of Nogo molecule in CNS.
authors.affiliation: Department of Immunology,Fourth Military Medical University,Xi'an 710032, China.
authors.forename: Chao-jun
authors.initials: CJ
authors.lastname: Song
authors.forename: Xi-ping
authors.initials: XP
authors.lastname: Cheng
authors.forename: Xue-song
authors.initials: XS
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhu
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Xiao-guang
authors.initials: XG
authors.lastname: Xu
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Bo-quan
authors.initials: BQ
authors.lastname: Jin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D005260:Female; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009185:Myelin Proteins / Q000032:analysis / Q000276:immunology*; D000070798:Nogo Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D009185:Myelin Proteins; D000070798:Nogo Proteins; C000603201:Rtn4 protein, mouse; C000603197:Rtn4 protein, rat