Catalase deficiency drastically affects gene expression induced by high light in Arabidopsis thaliana.
In plants, hydrogen peroxide (H(2)O(2)) plays a major signaling role in triggering both a defense response and cell death. Increased cellular H(2)O(2) levels and subsequent redox imbalances are managed at the production and scavenging levels. Because catalases are the major H(2)O(2) scavengers that remove the bulk of cellular H(2)O(2), altering their levels allows in planta modulation of H(2)O(2) concentrations. Reduced peroxisomal catalase activity increased sensitivity toward both ozone and photorespiratory H(2)O(2)-induced cell death in transgenic catalase-deficient Arabidopsis thaliana. These plants were used as a model system to build a comprehensive inventory of transcriptomic variations, which were triggered by photorespiratory H(2)O(2) induced by high-light (HL) irradiance. In addition to an H(2)O(2)-dependent and -independent type of transcriptional response during light stress, microarray analysis on both control and transgenic catalase-deficient plants, exposed to 0, 3, 8, and 23 h of HL, revealed several specific regulatory patterns of gene expression. Thus, photorespiratory H(2)O(2) has a direct impact on transcriptional programs in plants.
Vandenabeele
Steven
S
Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, Technologiepark 927, B-9052 Ghent, Belgium.
Vanderauwera
Sandy
S
Vuylsteke
Marnik
M
Rombauts
Stephane
S
Langebartels
Christian
C
Seidlitz
Harald K
HK
Zabeau
Marc
M
Van Montagu
Marc
M
Inzé
Dirk
D
Van Breusegem
Frank
F
D017360:Arabidopsis / Q000235:genetics / Q000528:radiation effects*; D029681:Arabidopsis Proteins; D016923:Cell Death / Q000528:radiation effects; D003470:Culture Media; D015870:Gene Expression / Q000528:radiation effects*; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008027:Light; D020411:Oligonucleotide Array Sequence Analysis; D010126:Ozone / Q000633:toxicity; D020675:Peroxisomes / Q000201:enzymology; D018515:Plant Leaves / Q000201:enzymology / Q000528:radiation effects; D030821:Plants, Genetically Modified; D011506:Proteins / Q000378:metabolism*; D015398:Signal Transduction
D029681:Arabidopsis Proteins; D003470:Culture Media; D011506:Proteins; D010126:Ozone; D006861:Hydrogen Peroxide; C490276:CAT2 protein, Arabidopsis