[Urotensin II induces hypertrophy of in vitro cultured neonatal rat cardiac myocytes].
OBJECTIVE
To examine whether urotensinII (UII) induces hypertrophy of neonatal rat cardiomyocytes cultured in vitro.


METHODS
The primary cardiac myocytes cultured for 40 h followed by further culture in serum-free media for another 24 h were subjected to exposure to UII of varied concentrations for 24 h, after which the changes in the size of the cells were analyzed by flow cytometry with (3)H-leucine incorporation also measured.


RESULTS
At the concentration of 1x10(-7) mol/L, UIIcould increase the size of the cultured cardiac myocardial cells (P=0.021) and 3H-Leucine incorporation (P=0.015).


CONCLUSIONS
UII may induce hypertrophy of neonatal rat cardiac myocytes cultured in vitro.
Zuo
Hui-hua
HH
Department of Cardiology, Guangzhou Red-Cross Hospital and the Fourth Hospital of Jinan University, Guangzhou 510220, China. hhzuo2002@sohu.com
Hong
Jie-min
JM
Guo
Heng-shan
HS
Wei
Jian-rui
JR
He
Yan-hua
YH
Li
Zhi-liang
ZL
D000818:Animals; D000831:Animals, Newborn; D006332:Cardiomegaly / Q000139:chemically induced*; D002478:Cells, Cultured; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D043562:Receptors, G-Protein-Coupled / Q000032:analysis; D014579:Urotensins / Q000633:toxicity*
D043562:Receptors, G-Protein-Coupled; D014579:Urotensins; C473142:Uts2r protein, rat; C029677:urotensin II