Nonaxoplasmic transfer of indocyanine green into the optic nerve after intravitreal application.
OBJECTIVE
To investigate whether indocyanine green (ICG) migrates into the optic nerve after intravitreal application in the rabbit eye.


METHODS
Gas vitrectomy was performed in one eye of adult Dutch belted rabbits by pars plana injection of 0.4 ml of 100% C3F8 (n = 10). One week later, vitrectomy was performed and 0.2 ml of 0.25% ICG was instilled into the vitreous cavity of these vitrectomized eyes. After 30 seconds of ICG application, the vitreous cavity was rinsed with balanced salt solution plus. Unoperated rabbits served as controls (n = 2). Globes, optic nerves, and the entire brain were removed, and ICG fluorescence of the specimens was examined with a digital fundus camera starting from the first day to the fourth postoperative week. Other rabbit eyes (n = 2) were injected with ICG directly into the vitreous without vitrectomy, and assessed for ICG signal after 1 day of ICG application. In the second part of the experiment, four rabbit eyes were gas vitrectomized and ICG was instilled onto retina after blockage of axonal flow by vinblastine. They were evaluated on the first (n = 2) and seventh postoperative days (n = 2).


RESULTS
No fluorescein was detected in the visual pathways and the brains of the control group of rabbits. The optic nerves of the ICG-instilled eyes were stained diffusely with ICG starting from the first day after surgery. ICG injected nonvitrectomized eyes showed similar ICG staining patterns when compared with vitrectomized eyes. ICG staining pattern of the optic nerve did not differ in vinblastine-pretreated eyes. Fluorescence extended along the intraorbital portion of the optic nerve but did not transport more posteriorly in all animals. At the fourth week after vitrectomy, ICG signal was still detectable, but staining was less intense.


CONCLUSIONS
Intravitreal ICG injection results in nonaxoplasmic extension into the optic nerve in the rabbit.
Cekiç
Osman
O
Department of Ophthalmology, Osaka University Medical School, Japan.
Morimoto
Takeshi
T
Ohji
Masahito
M
Sawa
Miki
M
Hasegawa
Takuya
T
Sakaguchi
Hirokazu
H
Ikuno
Yasushi
Y
Gomi
Fumi
F
Kamei
Motohiro
M
Tano
Yasuo
Y
D000818:Animals; D001370:Axonal Transport / Q000187:drug effects; D001692:Biological Transport; D004396:Coloring Agents / Q000493:pharmacokinetics*; D015590:Fluorophotometry; D007208:Indocyanine Green / Q000493:pharmacokinetics*; D007267:Injections; D009900:Optic Nerve / Q000378:metabolism*; D011817:Rabbits; D014747:Vinblastine / Q000494:pharmacology; D014821:Vitrectomy; D014822:Vitreous Body / Q000378:metabolism*
D004396:Coloring Agents; D014747:Vinblastine; D007208:Indocyanine Green