Lactate production in an integrated process configuration: reducing cell adsorption by shielding of adsorbent.
The problem of binding of microbial cells to an adsorbent matrix during in situ recovery of bioproducts from a fermentation broth has been addressed by shielding the adsorbent with a thin layer of a non-ionic polymer. Extractive bioconversion of lactic acid by integrating ion-exchange adsorption with the fermentation stage was studied. The effect of coating of the ion-exchanger with agarose on product recovery and cell adsorption was evaluated. Extractive fermentation with both uncoated and coated resin resulted in an increase in reactor productivity as compared to the normal fermentation. The free cell density in the system with agarose-coated beads was similar to that in control fermentation, but was significantly lower in the system with the uncoated ion-exchanger. Electron microscopic scanning of the bead surface after passage of the fermentation broth showed cells attached to the native adsorbent but not to the coated one.
Senthuran
Appadurai
A
Department of Biotechnology, Centre for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, 221 00 Lund, Sweden.
Senthuran
Vasanthe
V
Hatti-Kaul
Rajni
R
Mattiasson
Bo
B
D001422:Bacterial Adhesion; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D018914:Cells, Immobilized / Q000378:metabolism*; D005285:Fermentation; D007475:Ion Exchange Resins / Q000737:chemistry; D019344:Lactic Acid / Q000096:biosynthesis*; D007780:Lacticaseibacillus casei / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D011108:Polymers; D012685:Sepharose / Q000737:chemistry
D007475:Ion Exchange Resins; D011108:Polymers; D019344:Lactic Acid; C050187:amberlite IRA 400; D012685:Sepharose