Synthetic lipopeptide adjuvants and Toll-like receptor 2--structure-activity relationships.
Bacterial lipoproteins and their synthetic analogues (sLP) are strong immune modulators of the early host responses after infection. Synthetic lipopeptides are strong adjuvants for the adaptive immune system. Lipoproteins and lipopeptides induce signalling in immune cells through Toll-like receptor-TLR2/TLR1 heterodimers. By screening a combinatorial lipohexapeptide amide collection in an in vitro IL-8 induction assay, we systematically evaluated the potential of 19 proteinogenic amino acids in the peptide moiety of Pam3Cys-lipopeptides to interact with TLR2. New Pam3Cys-lipopeptides with high activity were obtained. Different fatty acids were introduced to investigate the influence of the acyl moiety. Lipopeptides with modifications in the core structure of the unusual amino acid S-glycerylcysteine were synthesized and tested for IL-8 induction via TLR2.
Spohn
Renate
R
EMC microcollections GmbH, Sindelfinger Street 3, 72070 Tuebingen, Germany. spohn@microcollections.de
Buwitt-Beckmann
Ute
U
Brock
Roland
R
Jung
Günther
G
Ulmer
Artur J
AJ
Wiesmüller
Karl-Heinz
KH
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D002460:Cell Line; D006801:Humans; D008074:Lipoproteins / Q000276:immunology* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000276:immunology*; D010455:Peptides / Q000276:immunology / Q000302:isolation & purification; D011956:Receptors, Cell Surface / Q000276:immunology*; D013329:Structure-Activity Relationship; D051194:Toll-Like Receptor 1; D051195:Toll-Like Receptor 2; D051193:Toll-Like Receptors
D000276:Adjuvants, Immunologic; D008074:Lipoproteins; D008562:Membrane Glycoproteins; D010455:Peptides; D011956:Receptors, Cell Surface; C495342:TLR2 protein, human; D051194:Toll-Like Receptor 1; D051195:Toll-Like Receptor 2; D051193:Toll-Like Receptors