A TMV-Cg mutant with a truncated coat protein induces cell death resembling the hypersensitive response in Arabidopsis.
Tobacco mosaic virus (TMV)-Cg is able to propagate and multiply systemically to high levels in Arabidopsis thaliana ecotype Col-0. In this study, we obtained a Cg mutant, Cgk1, which expresses a coat protein with a truncated carboxyl terminus. Interestingly, Cgk1 induced necrosis that resembled the hypersensitive response and caused more pronounced disease symptoms than wild type Cg in Arabidopsis Col-0 plants. A reactive oxidative burst occurred prior to this necrosis. We found that expression of the pathogenesis-related gene PR-1 was induced by Cgk1 infection, and also by infection with wild type Cg, but only in npr1-2 mutant plants, not in NahG transgenic plants. These results suggested that PR-1 expression is dependent on the salicylic acid signaling pathway, but is independent of NPR1.
Kurihara
Yukio
Y
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo 153-8902, Japan.
Watanabe
Yuichiro
Y
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000502:physiology* / Q000821:virology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D016923:Cell Death / Q000502:physiology*; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D016897:Respiratory Burst; D016415:Sequence Alignment; D014027:Tobacco Mosaic Virus / Q000235:genetics* / Q000378:metabolism
D029681:Arabidopsis Proteins; D036022:Capsid Proteins; C077262:PR-1 protein, Arabidopsis