Induction of CD28 on the new myeloma cell line MOLP-8 with t(11;14)(q13;q32) expressing delta/lambda type immunoglobulin.
The novel multiple myeloma (MM) cell line MOLP-8 carrying the t(11;14) (q13;q32) was established from the peripheral blood of a 52-year-old Japanese male patient with Bence-Jones delta/lambda type MM (stage IIIA with hyperammonemia). The growth of MOLP-8 cells is constitutively independent of exogenous growth factors or feeder cells. MOLP-8 cells grow mainly as free floating single cells and slightly adherent on the bottom of the plastic culture flask. Wright-Giemsa-stained MOLP-8 cells show the typical plasma cell morphology with abundant cytoplasm, heterogeneous cell size and one to three nuclei. The immunoprofile of MOLP-8 corresponds to that seen typically in primary MM cells: positive for cytoplasmic immunoglobulin (Ig) delta/lambda chains, CD10, CD29, CD38, CD40, CD44, CD49b, CD49d, CD54, CD56, CD58, CD71, CD138 and PCA-1; the cells were negative for surface Igs and various other B-cell, T-cell and myelomonocyte-associated immunomarkers. CD28 became positive after co-culture of MOLP-8 cells with bone marrow adherent stromal (BST) feeder cells for a week. About 30% of MOLP-8 cells adhered strongly to the BST cells, but the cellular adhesion was clearly inhibited by addition of either anti-CD29 or anti-CD106 monoclonal antibody, suggesting a specific cellular adhesion through alpha4beta1-integrin-VCAM-1 interaction. The novel MOLP-8 cell line together with the present myeloma cell lines will present useful model systems in the investigation of the biology of MM.
Matsuo
Yoshinobu
Y
Fujisaki Cell Center, Hayashibara Biochemical Labs., Inc., 675-1 Fujisaki, Okayama 702-8006, Japan. yomatsuo@hayashibara.co.jp
Drexler
Hans G
HG
Harashima
Akira
A
Okochi
Ayumi
A
Hasegawa
Atsuhiko
A
Kojima
Kensuke
K
Orita
Kunzo
K
D015703:Antigens, CD / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D018106:CD28 Antigens / Q000378:metabolism*; D002448:Cell Adhesion; D002455:Cell Division; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D006801:Humans; D007138:Immunoglobulin delta-Chains / Q000378:metabolism*; D007146:Immunoglobulin lambda-Chains / Q000378:metabolism*; D016023:Integrins / Q000378:metabolism; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000235:genetics / Q000276:immunology / Q000473:pathology; D010950:Plasma Cells / Q000473:pathology; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D014178:Translocation, Genetic / Q000235:genetics*; D014407:Tumor Cells, Cultured; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
D015703:Antigens, CD; D000954:Antigens, Surface; D018106:CD28 Antigens; D007138:Immunoglobulin delta-Chains; D007146:Immunoglobulin lambda-Chains; D016023:Integrins; D019010:Vascular Cell Adhesion Molecule-1