Effect of W07-toxin on gut physiological response in mice.
A number of unknown secretogenic factor(s) from Vibrio cholerae have been implicated to play a role in inducing cholera-like symptoms observed in patients. The present study has been carried out on the novel W07-toxin (pI 5.2) from V. cholerae W07, an epidemic cholera strain devoid of the ctx gene. The toxin showed maximum binding to GM(1) and interacted with a 20 kDa glycoprotein present on the cell membrane of mice enterocytes in a GM(1) specific manner. The analysis of biochemical parameters in enterocytes triggered with this toxin revealed a significant increase in intracellular calcium concentration and a massive secretion of Cl(-). However, no absorption of Na(+) was observed under the same condition. This toxin also elevated the level of cyclic adenosine 3',5'-monophosphate (cAMP) as well as protein kinase A (PKA). Thus, the novel toxin, although distinct from cholera-toxin, showed some functional homology to it and may be one of the key players inducing electrolyte imbalance within intestinal cells in the cholera-like symptoms associated with V. cholerae W07.
Bhattacharyya
Shalmoli
S
Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education and Research, Chandigarh 160012, India. shalmoli@glide.net.in
Ghosh
Sujata
S
Shant
Jasleen
J
Ganguly
Nirmal K
NK
Majumdar
Siddhartha
S
D000818:Animals; D001427:Bacterial Toxins / Q000302:isolation & purification / Q000378:metabolism / Q000633:toxicity*; D002118:Calcium / Q000032:analysis; D002712:Chlorides / Q000032:analysis; D000242:Cyclic AMP / Q000032:analysis; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D003593:Cytoplasm / Q000737:chemistry; D020895:Enterocytes / Q000187:drug effects* / Q000378:metabolism*; D005677:G(M1) Ganglioside / Q000378:metabolism; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D011485:Protein Binding; D012964:Sodium / Q000032:analysis; D014734:Vibrio cholerae / Q000302:isolation & purification / Q000472:pathogenicity*
D001427:Bacterial Toxins; D002712:Chlorides; D008562:Membrane Glycoproteins; C420689:Vibrio cholerae WO7 toxin; D005677:G(M1) Ganglioside; D012964:Sodium; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D002118:Calcium