title: Distribution of Kaposi sarcoma-associated herpesvirus/human herpesvirus 8 in maternal saliva and breast milk in Zambia: implications for transmission.
abstract: BACKGROUND
  The seroprevalence of Kaposi sarcoma-associated herpesvirus (KSHV)/human herpesvirus 8 (HHV-8) in sub-Saharan Africa suggests that multiple routes of transmission exist. In the present study, we examined 2 possible routes of mother-to-child transmission, through breast milk and saliva, during the first 6 months after delivery.
  
  
  METHODS
  The prevalence of HHV-8 DNA in the breast-milk cells (n=75), milk supernatant (n=56), colostrum (n=2), and saliva cells (n=65) of HHV-8-seropositive mothers who recently gave birth was examined. Polymerase chain reaction analysis was performed for the detection of HHV-8 in cross-sectional samples isolated at 2, 4, and 6 months after delivery.
  
  
  RESULTS
  None of the 75 breast-milk samples but 2 of the colostrum samples that were analyzed contained HHV-8 DNA at a limit of detection of approximately 1 HHV-8 copy/10(4) cellular genomes, whereas Epstein-Barr virus DNA and HIV-1 DNA were detected in 16 and 22 samples, respectively. Analysis of 65 saliva cell samples, which were obtained from mothers who also provided milk samples, revealed that 19 of the samples had detectable HHV-8 DNA. Viral DNA was found at all time points, but the presence of viral DNA in saliva was independent of maternal HIV-1 serostatus (chi 2=0.33; P=.57).
  
  
  CONCLUSIONS
  Our findings demonstrate the lack of HHV-8 DNA in the breast milk of seropositive mothers, and they suggest that contact with breast milk is not a likely source of horizontal transmission of virus to infants in sub-Saharan Africa.
authors.affiliation: Nebraska Center for Virology and School of Biological Sciences, University of Nebraska, Lincoln, Nebraska 68588-0666, USA.
authors.forename: Brad P
authors.initials: BP
authors.lastname: Brayfield
authors.forename: Chipepo
authors.initials: C
authors.lastname: Kankasa
authors.forename: John T
authors.initials: JT
authors.lastname: West
authors.forename: Jubra
authors.initials: J
authors.lastname: Muyanga
authors.forename: Ganapati
authors.initials: G
authors.lastname: Bhat
authors.forename: Winslow
authors.initials: W
authors.lastname: Klaskala
authors.forename: Charles D
authors.initials: CD
authors.lastname: Mitchell
authors.forename: Charles
authors.initials: C
authors.lastname: Wood
mesh_terms: D000595:Amino Acid Sequence; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006566:Herpesviridae Infections / Q000635:transmission* / Q000821:virology; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D018445:Infectious Disease Transmission, Vertical; D008895:Milk, Human / Q000821:virology*; D010802:Phylogeny; D012463:Saliva / Q000821:virology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; C111600:K1 protein, Human herpesvirus 8; D014764:Viral Proteins