Glutathione content and adaptation to endogenously induced energy depletion in Mv1Lu cells.
Transfection of genes that code for enzymes of energy metabolism provides alternative models to study the adaptive response to energy restriction induced by endogenous changes instead of by unfavorable environmental conditions. Overexpression of the glycolytic enzyme fructose-2,6-bisphosphatase reduced the content of fructose 2,6-bisphosphate, inducing energy limitation in the mink lung epithelial cell line Mv1Lu. This metabolic stress reduced the ATP available in transfected cells by 20%, which downregulated active ion transport and protein turnover. Ion homeostasis and cell function require concomitant reductions in cell membrane ion permeability and protein damage. Our results indicate that glutathione content linked these features of the adaptive response to the endogenously induced metabolic downregulation.
Boada
Jordi
J
Departament de Ciències Fisiològiques II, Universitat de Barcelona, E-08907 Barcelona, Spain.
Cuesta
Eduardo
E
Perales
Jose C
JC
Roig
Teresa
T
Bermudez
Jordi
J
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D015536:Down-Regulation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells / Q000378:metabolism; D005609:Free Radicals; D005947:Glucose / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D006019:Glycolysis; D007477:Ions; D008168:Lung / Q000166:cytology; D008564:Membrane Potentials; D008907:Mink; D009249:NADP / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology; D010101:Oxygen Consumption; D025481:Phosphofructokinase-2 / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D015221:Potassium Channels / Q000378:metabolism; D017382:Reactive Oxygen Species; D012414:Rubidium Radioisotopes / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014162:Transfection
D004791:Enzyme Inhibitors; D005609:Free Radicals; D007477:Ions; D015221:Potassium Channels; D017382:Reactive Oxygen Species; D012414:Rubidium Radioisotopes; D009249:NADP; D010042:Ouabain; D000255:Adenosine Triphosphate; D025481:Phosphofructokinase-2; D000254:Sodium-Potassium-Exchanging ATPase; D005978:Glutathione; D005947:Glucose; D011188:Potassium