Mucosal immunization against dental caries with plasmid DNA encoding pac gene of Streptococcus mutans in rats.
Salivary secretory immunoglobulin A (S-IgA) antibodies act as the first line of defense against dental caries by blocking of adherence of Streptococcus mutans to tooth surfaces. This study focused on finding proper mucosal immunization route and delivery system to induce higher level of specific anti-S. mutans saliva S-IgA and inhibit dental caries in animal model. By immunizing rats with an anti-caries DNA vaccine, pCIA-P, via different mucosal routes, we found that intranasal (i.n.) immunization with pCIA-P/bupivacaine DNA complexes elicited the highest specific anti-S. mutans saliva S-IgA mucosal antibody responses compared with naked DNA and other routes. Correspondingly, rats immunized with pCIA-P/bupivacaine DNA complex via i.n. displayed the least carious lesions. Our findings suggested that DNA vaccination via intranasal immunization with bupivacaine delivery system be a promising approach against dental caries.
Jia
Rong
R
Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, PR China.
Guo
Ji Hua
JH
Fan
Ming Wen
MW
Bian
Zhuan
Z
Chen
Zhi
Z
Peng
Bin
B
Fan
Bing
B
D000281:Administration, Intranasal; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D003731:Dental Caries / Q000517:prevention & control*; D005822:Genetic Vectors / Q000235:genetics; D007114:Immunization; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008562:Membrane Glycoproteins / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013295:Streptococcus mutans / Q000235:genetics* / Q000276:immunology; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use*
D001426:Bacterial Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D008562:Membrane Glycoproteins; C055764:S-layer proteins; D019444:Vaccines, DNA