[Detection of West Nile virus in birds in the territories of Baraba and Kulunda lowlands (West Siberian migration way) during summer-autumn of 2002].
West Nile Virus (WNV) was discovered in 3 species of birds collected in summer-autumn, 2002, in the South of Western Siberia. WNV was identified by ELISA and RT-PCR. Three of 5 dead rooks (Corvus frugilegus), which were found in the territory of the Kulunda plain, were WNV-infected. WNV RNA was detected in 2% of samples of internal organs of aquatics birds, i.e. teal (Anas crecca) and garganey (Anas querquedula), caught in the Chany Lake (Baraba plain). Nucleotide sequencing of the 300-472 aa fragment of WNV protein E gene showed the maximum level of homology with strain WNV/LEIV-Vlg99-27889, which was isolated from a patient in Volgograd (1999). A high homology level of nucleotide sequencing denotes the relationship between the WNV circulating in the Northern Caspian Region and in the South of Western Siberia.
Ternovoĭ
V A
VA
Shchelkanov
M Iu
MIu
Shestopalov
A M
AM
Aristova
V A
VA
Protopopova
E V
EV
Gromashevskiĭ
V L
VL
Druziaka
A V
AV
Slavskiĭ
A A
AA
Zolotykh
S I
SI
Loktev
V B
VB
L'vov
D K
DK
D000595:Amino Acid Sequence; D000818:Animals; D000835:Animals, Wild / Q000821:virology*; D000956:Antigens, Viral / Q000032:analysis; D001717:Birds / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012621:Seasons; D016415:Sequence Alignment; D012800:Siberia; D014902:West Nile virus / Q000235:genetics / Q000302:isolation & purification*
D000956:Antigens, Viral; D012367:RNA, Viral