title: Disappearance of the PHA-E lectin binding site on the surface of ejaculated sperm and sperm capacitation in the dog.
abstract: Ejaculated semen and cross sections of the cauda epididymides collected from 3 normal dogs were smeared or stamped on glass slides, and the sperm on the slides were stained with 7 different FITC-lectins (Con A, DBA, GS-1, PHA-E, PSA, UEA-1, WGA) to examine the relation between sperm-binding glycoprotein derived from the canine prostate and sperm capacitation. The only lectin that stained the ejaculated sperm but not the cauda epididymal sperm was PHA-E. The sperm ejaculated by 5 other dogs were incubated for 4 hr in fluid flushed from the uterine horns or oviducts of estrous bitches, and then the percentages of actively motile sperm and hyperactivated sperm (HA-sperm) were determined. The percentages of PHA-E-labeled sperm and sperm labeled with fluoresceinated Ca indicator to assess the influx of Ca into the sperm were also evaluated. The mean percentages of actively motile sperm, HA-sperm, and Ca-labeled sperm after 4 hr of incubation in the uterine flush fluid and oviductal flush fluid were significantly higher than in control medium (P<0.05, 0.01), but the mean percentages of PHA-E-labeled sperm were lower (both P<0.01). The percentages of PHA-E-unlabeled sperm correlated with the percentages of both HA-sperm and Ca-labeled sperm (r(2)=0.787 and 0.812, respectively). The results indicate that loss of the glycoprotein secreted by the canine prostate on the sperm surface induces the influx of Ca into the sperm, and then hyperactivation of the sperm.
authors.affiliation: Department of Reproduction, Nippon Veterinary and Animal Science University, Tokyo, Japan.
authors.forename: Eiichi
authors.initials: E
authors.lastname: Kawakami
authors.forename: Takashi
authors.initials: T
authors.lastname: Sato
authors.forename: Taichi
authors.initials: T
authors.lastname: Hirano
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Hori
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Tsutsui
mesh_terms: D020101:Acrosome Reaction / Q000187:drug effects / Q000502:physiology; D000818:Animals; D001665:Binding Sites; D001693:Biological Transport, Active / Q000187:drug effects; D001826:Body Fluids / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D004285:Dogs / Q000378:metabolism* / Q000502:physiology; D004822:Epididymis / Q000166:cytology; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism / Q000494:pharmacology; D037102:Lectins / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D011485:Protein Binding; D013075:Sperm Capacitation / Q000502:physiology*; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology; D013094:Spermatozoa / Q000378:metabolism* / Q000502:physiology
chemical_list: D006025:Glycosaminoglycans; D037102:Lectins; D008562:Membrane Glycoproteins; D016650:Fluorescein-5-isothiocyanate; D002118:Calcium