title: The determination of deoxyribonucleic acids with triadimenol based on the enhancement of resonance light scattering.
abstract: For the first time, triadimenol was used to determine nucleic acid (DNA) using the resonance light scattering (RLS) technique. The RLS of triadimenol was greatly enhanced by DNA in the range of pH 1.6 to approximately 1.9. A resonance light-scattering peak at 310 nm was found, and the enhanced intensity of RLS at this wavelength was proportional to the concentration of DNA. The linear range of the calibration curve was 0 to approximately 9 microg/ml with the detection limit of 24 ng ml(-1). The mechanism studies of the system indicated that the enhanced RLS is due to the aggregation of triadimenol on DNA. The nucleic acids in synthetic samples and in rice seedling extraction were analyzed with satisfactory results. Compared with other methods, this method is convenient, rapid, inexpensive and simple.
authors.affiliation: College of Applied Chemistry, China Agricultural University, Beijing, PR China. aydy-826@163.com
authors.forename: Nianqin
authors.initials: N
authors.lastname: Jie
authors.forename: Shicong
authors.initials: S
authors.lastname: Hou
authors.forename: Fengpei
authors.initials: F
authors.lastname: Du
authors.forename: Chunrong
authors.initials: C
authors.lastname: Zhang
authors.forename: Guoxin
authors.initials: G
authors.lastname: Qin
mesh_terms: D004247:DNA / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D012015:Reference Standards; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D014230:Triazoles
chemical_list: D014230:Triazoles; C032910:triadimefon; D004247:DNA