title: [Inhibitory effect of hDAF displayed on yeast cells on deposition of complement on the yeast cells].
abstract: OBJECTIVE
  To investigate whether the hDAF displayed on the surface of yeast cells can inhibit the deposition of C5b-9.
  
  
  METHODS
  After treatment with normal human serum, deposition of human complement C5b-9 and DAF expression on yeast cells EBY100 were analyzed by flow cytometry.
  
  
  RESULTS
  Yeast cells EBY100 could activate the complement molecules in human serum and lead to the deposition of C5b-9, while the deposition of C5b-9 on the surface of yeast cells with hDAF could significantly reduce the deposition of C5b-9.
  
  
  CONCLUSIONS
  hDAF displayed on the surface of yeast cells can fold correctly and exhibit activity of complement's inhibition.
authors.affiliation: Department of Immunology, Third Military Medical University, Chongqing 400038, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Guo
authors.forename: Ping
authors.initials: P
authors.lastname: Zheng
authors.forename: Pei-rong
authors.initials: PR
authors.lastname: Xie
authors.forename: Yu-zhang
authors.initials: YZ
authors.lastname: Wu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zou
mesh_terms: D018958:CD55 Antigens / Q000378:metabolism*; D002478:Cells, Cultured; D003167:Complement Activation; D015938:Complement Membrane Attack Complex / Q000378:metabolism*; D006801:Humans; D017510:Protein Folding; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
chemical_list: D018958:CD55 Antigens; D015938:Complement Membrane Attack Complex