Toward fingerprinting cellular lipidomes directly from biological samples by two-dimensional electrospray ionization mass spectrometry.
Cellular lipids are composed of linear combinations of aliphatic chains covalently attached to backbones and/or head groups that collectively represent the cellular lipidome. Herein we present a novel two-dimensional electrospray ionization mass spectrometric approach to fingerprint most of the major and many of the minor lipid classes in the hepatic cellular lipidome, which collectively represent >80% of the total lipid mass, directly from their chloroform extracts. Through lipid class-selective intrasource ionization and subsequent analysis of two-dimensional cross-peak intensities, the chemical identity and mass composition of individual molecular species of most mouse hepatic lipid classes were determined from its chloroform extract. This new integrated platform provides a robust foundation for the automated analysis of multidimensional mass spectra to advance the level of analytical scripting in lipidomics to a new plateau.
Han
Xianlin
X
Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St. Louis, MO 63110, USA. xianlin@wustl.edu
Yang
Jingyue
J
Cheng
Hua
H
Ye
Hongping
H
Gross
Richard W
RW
D000818:Animals; D005591:Chemical Fractionation; D002725:Chloroform; D008055:Lipids / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D008099:Liver / Q000737:chemistry; D008297:Male; D051379:Mice; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D008055:Lipids; D002725:Chloroform