Columnar resolution of blood volume and oximetry functional maps in the behaving monkey; implications for FMRI.
The ultimate goal of high-resolution functional brain mapping is single-condition (stimulus versus no-stimulus maps) rather than differential imaging (comparing two "stimulus maps"), because the appropriate ("orthogonal") stimuli are rarely available. This requires some component(s) of activity-dependent hemodynamic signals to closely colocalize with electrical activity, like the early increase in deoxyhemoglobin, shown previously to yield high-quality functional single-condition maps. Conversely, nonlocal vascular responses dominate in cerebral blood volume (CBV)-based single-condition maps. Differential CBV maps are largely restricted to the parenchyma, implying that part of the CBV response does colocalize with electrical activity at fine spatial scale. By removing surface vascular activation from optical imaging data, we document the existence of a capillary CBV response component, regulated at fine spatial scale and yielding single-condition maps exhibiting approximately 100 microm resolution. Blood volume and -flow based single-condition functional mapping at columnar level should thus be feasible, provided that the capillary response component is selectively imaged.
Vanzetta
Ivo
I
Department of Neurobiology, The Weizmann Institute of Science, 76100 Rehovot, Israel. vanzetta@incm.cnrs-mrs.fr
Slovin
Hamutal
H
Omer
David B
DB
Grinvald
Amiram
A
D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology; D001810:Blood Volume; D001931:Brain Mapping; D002540:Cerebral Cortex / Q000098:blood supply* / Q000502:physiology; D002560:Cerebrovascular Circulation / Q000502:physiology*; D003287:Contrast Media / Q000378:metabolism; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008252:Macaca fascicularis; D008279:Magnetic Resonance Imaging; D008297:Male; D010092:Oximetry; D010100:Oxygen / Q000032:analysis*; D010775:Photic Stimulation / Q000379:methods; D011930:Reaction Time / Q000502:physiology; D013997:Time Factors; D014851:Wakefulness / Q000502:physiology
D003287:Contrast Media; D010100:Oxygen