title: Truncated mouse adenomatous polyposis coli reduces connexin32 content and increases matrilysin secretion from Paneth cells.
abstract: Heterozygous mutations in adenomatous polyposis coli (APC) is an early event in inheritable and sporadic colon cancer development. We recently found reduced connexin (Cx43) expression in intestinal cell lines with heterozygous Apc mutation. In this study we investigated Cx expression and the role of one mutated Apc allele in epithelia of multiple intestinal neoplasia (Min) mouse intestines by immunohistochemistry. Cx43 was not expressed in intestinal epithelia of Min and wild-type mice. Cx32 was specifically expressed in enterochromaffin cells in both mice types, and in Paneth cells of wild-type mice. In contrast, Min mice had nearly undetectable level of Cx32 in Paneth cells. Isolated small intestinal crypts from Min mice had markedly increased secretion of both lysozyme and matrilysin compared with wild-type mice. Absence of matrilysin in Min mice reduces adenoma development. Reduced Cx32 and increased matrilysin secretion from Paneth cells could be important to neoplastic development in the intestine.
authors.affiliation: Department of Food Toxicology, Norwegian Institute of Public Health, P.O. Box 4404 Nydalen, NO-0403 Oslo, Norway. trine.husoy@fhi.no
authors.forename: Trine
authors.initials: T
authors.lastname: Husøy
authors.forename: Hege B
authors.initials: HB
authors.lastname: Ølstørn
authors.forename: Helle K
authors.initials: HK
authors.lastname: Knutsen
authors.forename: Else Marit
authors.initials: EM
authors.lastname: Løberg
authors.forename: Véronique
authors.initials: V
authors.lastname: Cruciani
authors.forename: Svein-Ole
authors.initials: SO
authors.lastname: Mikalsen
authors.forename: Ingeborg L
authors.initials: IL
authors.lastname: Goverud
authors.forename: Jan
authors.initials: J
authors.lastname: Alexander
mesh_terms: D011125:Adenomatous Polyposis Coli / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D018031:Connexin 43 / Q000378:metabolism*; D017630:Connexins / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D019879:Paneth Cells / Q000378:metabolism*; D000097002:Gap Junction beta-1 Protein
chemical_list: D018031:Connexin 43; D017630:Connexins; D020783:Matrix Metalloproteinase 7; D000097002:Gap Junction beta-1 Protein