title: Beta-tricalcium phosphate combined with recombinant human bone morphogenetic protein-2: a substitute for autograft, used for packing interbody fusion cages in the canine lumbar spine.
abstract: Beta-tricalcium phosphate (beta-TCP) combined with recombinant human bone morphogenetic protein-2 (BMP-2) was examined as a substitute for autograft for packing into interbody fusion cages in the canine lumbar spine model. Discectomy and interbody cage fusion were performed at three disc spaces in eight dogs. Examination of microradiographs and histological sections of the lumbar spine at 16 weeks postsurgery revealed three fusions in the autograft cages (Group A), three in the beta-TCP cages (Group B), and five in the beta-TCP-BMP-2 cages (Group C). The mean percentage of trabecular bone area in the cages was 51.9% in Group A, 48.8% in Group B, and 65.6% in Group C. Mean percentage of trabecular bone formation and mechanical stiffness were highest in the cages filled with beta-TCP and BMP-2. Combination of BMP to beta-TCP may act as an osteoconductive and osteoinductive bone graft substitute in clinical spine surgery.
authors.affiliation: Department of Neurosurgery, Mie University School of Medicine, Tsu, Mie, Japan. takashiro.ohyama@nifty.com
authors.forename: Takashiro
authors.initials: T
authors.lastname: Ohyama
authors.forename: Yoshichika
authors.initials: Y
authors.lastname: Kubo
authors.forename: Hiroo
authors.initials: H
authors.lastname: Iwata
authors.forename: Waro
authors.initials: W
authors.lastname: Taki
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000502:physiology*; D018786:Bone Substitutes / Q000008:administration & dosage*; D016025:Bone Transplantation; D002130:Calcium Phosphates / Q000008:administration & dosage*; D004285:Dogs; D004359:Drug Therapy, Combination; D016268:Internal Fixators; D008159:Lumbar Vertebrae / Q000601:surgery; D008297:Male; D011994:Recombinant Proteins; D013123:Spinal Fusion / Q000379:methods*; D016212:Transforming Growth Factor beta / Q000502:physiology*
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D018786:Bone Substitutes; D002130:Calcium Phosphates; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C485817:beta-tricalcium phosphate