[Participation of ICAM-1 in the cytotoxicity of TM-TNF-alpha mediated by TNF receptor I].
OBJECTIVE
To identify membrane-associated molecules involved in the tumoricidal cytotoxicity of TM-TNF-alpha and to explore the molecular mechanism underlying the tumoricidal cytotoxicity of TM-TNF-alpha and differences of biological effects between TM-TNF-alpha and sTNF.


METHODS
Antibody blocking test and RT-PCR were used to evaluate the role of ICAM-1 and VCAM-1 in the cytotoxicity of TM-TNF-alpha mediated by TNFR I or TNFR II.


RESULTS
After the ICAM-1 expressed on the membrane of MCF-7 cells(expressing TNFRI) was blocked, the cytotoxicity of TM-TNF-alpha to this cell lines decreased significantly, while blocking VCAM-1 had no effect. Blocking ICAM-1 or VCAM-1 expressed on the membrane of HL-60 cells(expressing TNFRII)had no significant effects on the cytotoxicity of TM-TNF-alpha to this cell lines.


CONCLUSIONS
ICAM-1 participates in the cytotoxicity of TM-TNF mediated by TNFRI.
Zheng
Fang
F
Institute of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. zhengfangtj@yahoo.com
Shi
Wen-fang
WF
Feng
Wei
W
Jiang
Xiao-dan
XD
Xu
Yong
Y
Fan
Fang
F
Li
Zhuo-ya
ZY
D000818:Animals; D015703:Antigens, CD / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology*; D051379:Mice; D018124:Receptors, Tumor Necrosis Factor / Q000502:physiology*; D047888:Receptors, Tumor Necrosis Factor, Type I; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000502:physiology
D015703:Antigens, CD; D018124:Receptors, Tumor Necrosis Factor; D047888:Receptors, Tumor Necrosis Factor, Type I; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1