title: Detection of Grb-2-related adaptor protein gene (GRAP) and peptide molecule in salivary glands of MRL/lpr mice and patients with Sjögren's syndrome.
abstract: The pathogenesis of Sjögren's syndrome (SS) is poorly understood. In this study we used an in-house mouse spleen cDNA microarray to analyse genes in spleens from MRL/lpr (an SS mouse model) mice. We have previously demonstrated that GRAP genes were up-regulated in salivary glands of the same mice. The microarray analysis showed that seven out of 2304 genes were highly expressed in spleens from the MRL/lpr mice, one of which was the GRAP gene. In other words, the GRAP gene is highly expressed in the salivary glands and spleen of MRL/lpr mice. We also carried out immunohistochemical studies. Mouse and human Grb-2-related adaptor protein (GRAP) antigens were expressed on ductal cells and infiltrating lymphocytes in salivary glands of MRL/lpr mice and SS patients, but only weakly in controls (MRL/+ mice and individuals with salivary cysts). These results suggest that the GRAP gene might have a role in the pathogenesis of SS.
authors.affiliation: First Department of Internal Medicine, Nihon University School of Medicine, Nerima Hikarigaoka Nihon University Hospital, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Shiraiwa
authors.forename: M
authors.initials: M
authors.lastname: Takei
authors.forename: T
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: T
authors.initials: T
authors.lastname: Azuma
authors.forename: M
authors.initials: M
authors.lastname: Kato
authors.forename: K
authors.initials: K
authors.lastname: Mitamura
authors.forename: T
authors.initials: T
authors.lastname: Ueki
authors.forename: A
authors.initials: A
authors.lastname: Kida
authors.forename: T
authors.initials: T
authors.lastname: Horie
authors.forename: N
authors.initials: N
authors.lastname: Seki
authors.forename: S
authors.initials: S
authors.lastname: Sawada
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D019463:Mice, Inbred MRL lpr; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D012469:Salivary Glands / Q000166:cytology / Q000473:pathology / Q000502:physiology*; D012859:Sjogren's Syndrome / Q000378:metabolism*; D013154:Spleen / Q000502:physiology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C409587:GRAP2 protein, human; C510602:Grap protein, human; C498726:Mona protein, mouse; D010455:Peptides