An assessment of different DNA delivery systems for protection against respiratory syncytial virus infection in the murine model: gene-gun delivery induces IgG in the lung.
Immunization with plasmid DNA (pDNA) has the potential to overcome the difficulties of neonatal vaccination that may be required for protection against infection with respiratory syncytial virus (RSV); however, little is known about optimal delivery modalities. In this pilot study we compared mucosal delivery of pDNA encoding RSV F protein encapsulated in poly(DL-lactide-co-glycolide) with delivery of pDNA by gene-gun for the induction of immunity in mice. Intra-gastric or intra-nasal immunization with various doses of microparticles induced weak low levels of RSV-specific serum antibodies in a proportion of mice; in contrast, gene-gun vaccination led to protective immunity associated with a humoral response. Interestingly, RSV-specific antibody was detected in lung fragment cultures following intradermal vaccination with the gene-gun.
Tree
Julia A
JA
Health Protection Agency, Porton Down, Salisbury, Wiltshire SP4 0JG, UK.
Bembridge
Gary
G
Hou
Sam
S
Taylor
Geraldine
G
Fashola-Stone
Elizabeth
E
Melero
José
J
Cranage
Martin P
MP
D000818:Animals; D002460:Cell Line; D016503:Drug Delivery Systems / Q000592:standards; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology*; D008171:Lung Diseases / Q000517:prevention & control / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology / Q000517:prevention & control*; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology
D007074:Immunoglobulin G; D019444:Vaccines, DNA; D014764:Viral Proteins