Resuscitation of a defective prophage in Salmonella cocultures.
Widely studied Salmonella enterica serovar Typhimurium strains ATCC 14028s and SL1344 harbor a cryptic ST64B prophage unable to produce infectious virions. We found that coculturing either strain with an isogenic sibling lacking the prophage leads to the appearance of active forms of the virus. Active phage originates from reversion of a +1 frameshift mutation at a monotonous G:C run in a presumptive tail assembly pseudogene.
Figueroa-Bossi
Nara
N
Centre de Génétique Moléculaire, CNRS, 91198 Gif-sur-Yvette cedex, France. figueroa@cgm.cnrs-gif.fr
Bossi
Lionello
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D016368:Frameshift Mutation; D008969:Molecular Sequence Data; D039002:Prophages / Q000235:genetics / Q000502:physiology*; D012483:Salmonella Phages / Q000235:genetics / Q000502:physiology*; D012486:Salmonella typhimurium / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics; D014775:Virus Activation
D014764:Viral Proteins