title: Genotype and animal infectivity of a human isolate of Cryptosporidium parvum in the Republic of Korea.
abstract: Cryptosporidium parvum oocysts were isolated from a child suffering from acute gastroenteritis and successfully passaged in a calf and mice (designated hereafter SNU-H1) in the Republic of Korea; its molecular genotype has been analyzed. The GAG microsatellite region was amplified by a polymerase chain reaction (PCR), with a 238 base pair product, which is commonly displayed in C. parvum. The isolate was shown to be a mixture of the genotypes 1 (anthroponotic) and 2 (zoonotic). To study its infectivity in animals, 2 calves and 3 strains of mice were infected with the SNU-H1; in these animals, the propagation of both genotypes was successful. In immunosuppressed (ImSP) BALB/c and C57BL/6 mice the number of oocysts decreased after day 10 post-infection (PI); but in ImSP ICR mice, they remained constant until day 27 PI. The results show that both the C. parvum genotypes 1 and 2 can be propagated in calves and ImSP mice.
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea.
authors.forename: Sang-Mee
authors.initials: SM
authors.lastname: Guk
authors.forename: Tai-Soon
authors.initials: TS
authors.lastname: Yong
authors.forename: Soon-Jung
authors.initials: SJ
authors.lastname: Park
authors.forename: Jae-Hwan
authors.initials: JH
authors.lastname: Park
authors.forename: Jong-Yil
authors.initials: JY
authors.lastname: Chai
mesh_terms: D000818:Animals; D002417:Cattle; D002648:Child; D003457:Cryptosporidiosis / Q000382:microbiology; D016785:Cryptosporidium parvum / Q000235:genetics* / Q000276:immunology; D003967:Diarrhea / Q000469:parasitology; D005243:Feces / Q000469:parasitology; D005838:Genotype; D006801:Humans; D007723:Korea; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D033661:Oocysts; D016133:Polymerase Chain Reaction; D015047:Zoonoses / Q000469:parasitology