[Expression of human growth hormone gene in mice and its effect on concentration of murine serum cytokines].
OBJECTIVE
To explore the effects of high level human growth hormone(GH) on immune function.


METHODS
The eukarytic expression plasmid containing hGH gene was constructed and then was injected into musculi quadriceps femoris of mice. 5th, 15th and 25th day after injection, the murine sera were collected and the levels of hGH, IL-2, IFN-gamma and TNF-alpha were detected by ELISA.


RESULTS
hGH cDNA was correctly cloned into pcDNA3. COS-1 cells transfected with the recombinant plasmid secreted high level of active hGH. The concentration of hGH in the sera of mice receiving the plasmid was obviously higher than that of control group. However, the concentrations of IL-2, IFN-gamma and TNF-alpha had no significant variation in comparison with those of control group.


CONCLUSIONS
High level GH has no marked effect on secretion of IL-2, IFN-gamma and TNF-alpha.
Song
Xiang-feng
XF
Department of Immunology, First Affiliated Hospital, Xinxiang Medical College, Xinxiang 453003, China. xfsong@xxmc.edu.cn
Zhang
Wen
W
Shi
Tai-xin
TX
Wang
Hui
H
D000818:Animals; D019556:COS Cells / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D019382:Human Growth Hormone / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D006801:Humans; D007371:Interferon-gamma / Q000097:blood*; D007376:Interleukin-2 / Q000097:blood*; D019169:Jurkat Cells / Q000166:cytology; D008297:Male; D051379:Mice; D010957:Plasmids; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D019382:Human Growth Hormone; D007371:Interferon-gamma