S-Adenosyl methionine/S-adenosyl-L-homocysteine ratio determination by capillary electrophoresis employed as a monitoring tool for the antiviral effectiveness of adenosine analogs.
S-Adenosyl-L-homocysteine hydrolase (SAHh) inhibitors have long been used as broad-range antivirals and have been recently evaluated as an experimental therapy of filovirus infections. In response to the need for a rapid laboratory testing method that could assess antiviral potency in vivo, our group developed a capillary electrophoresis (CE) method for the determination of the S-adenosyl-L-homocysteine (SAH) to S-adenosyl-L-methionine (SAM) ratio. After chloroacetaldehyde derivatization, SAH and SAM were detected using laser-induced fluorescence detection with a HeCd laser. Separation and quantitation of both SAH and SAM in human plasma were achieved in less than 1 min. The proposed method is rapid and reliable, and could be easily applied to routine monitoring of clinical and preclinical trials subjects.
Sbrana
Elena
E
Department of Pathology, University Of Texas Medical Branch, 1.104 Keiller Building, 301 University Boulevard, Galveston, TX 77555-0609, USA. elsbrana@utmb.edu
Bramanti
Emilia
E
Spinetti
Maria C
MC
Raspi
Giorgio
G
D000079:Acetaldehyde / Q000031:analogs & derivatives* / Q000737:chemistry*; D000998:Antiviral Agents / Q000494:pharmacology*; D019075:Electrophoresis, Capillary; D006801:Humans; D012435:S-Adenosylhomocysteine / Q000032:analysis* / Q000097:blood; D012436:S-Adenosylmethionine / Q000032:analysis* / Q000097:blood
D000998:Antiviral Agents; D012436:S-Adenosylmethionine; D012435:S-Adenosylhomocysteine; C004656:chloroacetaldehyde; D000079:Acetaldehyde