PCR-RFLP using a SNP on the mitochondrial Lsu-rDNA as an easy method to differentiate Tuber melanosporum (Perigord truffle) and other truffle species in cans.
Canned truffle products labeled Tuber melanosporum, the famous Perigord truffle, may contain other less tasty and cheaper truffle species. To protect consumers from fraud, a PCR DNA-based method was used to unequivocally identify the nature of the product. Several rapid and simple cell lysis procedures, used in conjunction with a commercially available DNA purification kit, were evaluated for their effectiveness in recovering DNA from canned truffle. In parallel, a marker for T. melanosporum was tested on the mitochondrial rDNA. These two techniques were then combined to differentiate T. melanosporum from other truffle species like T. aestivum, T. brumale or T. indicum up to the legal threshold in canned products. These findings not only allow a comparison of the effectiveness of the different DNA extraction methods but also provide a preliminary indication of the specificity and sensitivity of the detection with the mitochondrial marker that might be attainable for truffle species in a quantitative PCR-based analysis method.
Mabru
D
D
I.N.R.A., UMR 1095 INRA-UBP Amélioration et santé des plantes, Site de Crouelle, 234 avenue du Brézet, 63039 Clermont-Ferrand Cedex 2, France.
Douet
J P
JP
Mouton
A
A
Dupré
C
C
Ricard
J M
JM
Médina
B
B
Castroviejo
M
M
Chevalier
G
G
D001203:Ascomycota / Q000145:classification* / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005506:Food Contamination / Q000032:analysis* / Q000517:prevention & control; D005519:Food Preservation; D005524:Food Technology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity
D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal