Noninvasive pigment identification in single cells from living phototrophic biofilms by confocal imaging spectrofluorometry.
A new imaging technique for the analysis of fluorescent pigments from a single cell is reported. It is based on confocal scanning laser microscopy coupled with spectrofluorometric methods. The setup allows simultaneous establishment of the relationships among pigment analysis in vivo, morphology, and three-dimensional localization inside thick intact microbial assemblages.
Roldán
M
M
Departament de Productes Naturals, Biologia Vegetal i Edafologia, Universitat de Barcelona, E-08028 Barcelona, Spain.
Thomas
F
F
Castel
S
S
Quesada
A
A
Hernández-Mariné
M
M
D018441:Biofilms / Q000254:growth & development*; D000460:Chlorophyta / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D003470:Culture Media; D000458:Cyanobacteria / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D005453:Fluorescence; D007091:Image Processing, Computer-Assisted; D018613:Microscopy, Confocal / Q000379:methods; D010788:Photosynthesis; D010860:Pigments, Biological / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods
D003470:Culture Media; D010860:Pigments, Biological