[Study on therapeutic window of opportunity for Panax notoginseng saponins following focal cerebral ischemia/reperfusion injury in rats].
OBJECTIVE
To study the therapeutic window of opportunity for Panax notoginseng saponins (Pns) following focal cerebral ischemia/reperfusion injury in rats.


METHODS
Focal cerebral ischemia (2 h)/reperfusion (24 h) model in male rats was induced by transient occlusion and middle cerebral artery (MCA) for 2 h and reperfusion for 24 h. Drugs were administered at 3 h, 4 h, 5 h and 6 h after the onset of ischemia respectively and neurological deficit score, infarct size and brain edema were examined.


RESULTS
The administration of Pns at 3-4 h after onset of ischemia significantly reduced neurological deficit score, infarct size and brain edema. When administrating Pns at 5 h after onset of ischemia, the neuroprotection decreased. When administrating Pns at 6 h after onset of ischemia, there are not significant protective effects.


CONCLUSIONS
The therapeutic window of opportunity for Pns following focal cerebral ischemia/reperfusion injury in rats is not more than 5 h after the onset of ischemia.
He
Wei
W
Gannan Medical College, Ganzhou 341000.
Zhu
Zunping
Z
Liu
Jianxin
J
Ye
Heyang
H
Zeng
Jin
J
Huang
Xianhua
X
Lai
Fei
F
D000818:Animals; D002545:Brain Ischemia / Q000473:pathology*; D002544:Cerebral Infarction / Q000473:pathology; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D036145:Ginsenosides / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D008297:Male; D005894:Panax / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology*; D013997:Time Factors
D004365:Drugs, Chinese Herbal; D036145:Ginsenosides