Helicobacter pylori and malignant lymphoma in Spain.
Helicobacter pylori has been associated with gastric adenocarcinoma and gastric lymphoma. We report on the systematic evaluation of serologic detection of H. pylori in a lymphoma case-control study.


METHODS
Cases (N = 536) were consecutive patients newly diagnosed with a lymphoid malignancy between 1998 and 2002 in four centers in Spain. Lymphomas were diagnosed and classified using the WHO Classification. Controls (N = 603) were hospitalized patients frequency-matched to the cases by 5-year age group, sex, and study center. Severe immunocompromised patients were excluded as controls. Patients underwent a personal interview and blood sampling. H. pylori infection was evaluated by the presence of IgG antibodies using the Premier enzyme immunoassay kit (Meridian Diagnostics Inc., Cincinnati, OH). Logistic regression analysis was used to estimate the odds ratios and 95% confidence intervals (OR, 95% CI) for lymphoma categories.


RESULTS
Anti-H. pylori antibodies were detected in 68.5% of the cases and 71.3% of the controls (P = 0.29) H. pylori was associated with a 3-fold excess risk of splenic marginal B-cell lymphoma (OR = 3.97, 95% CI = 0.92-17.16). H. pylori was not associated with an overall increased risk of extranodal lymphomas (OR = 0.73, 95% CI = 0.44-1.22) but when specific sites were explored, the four mucosa-associated lymphoid tissue and the six diffuse large B-cell lymphomas primary localized in the stomach were all H. pylori seropositive.


CONCLUSIONS
Persistent infection with H. pylori may be implicated in the development of lymphomas of the gastric mucosa and of the spleen. These results could have clinical implications in the management of splenic marginal zone lymphomas.
de Sanjose
Silvia
S
Servei d'Epidemiologia & Registre del Cancer, Institut Catala d'Oncologia, Gran Via Km 2.7, 08907 L'Hospitalet, Barcelona, Spain. s.sanjose@ico.scs.es
Dickie
Andrew
A
Alvaro
Tomas
T
Romagosa
Vicens
V
Garcia Villanueva
Mercedes
M
Domingo-Domenech
Eva
E
Fernandez de Sevilla
Alberto
A
El-Omar
Emad
E
D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D016481:Helicobacter Infections / Q000097:blood / Q000150:complications / Q000453:epidemiology; D016480:Helicobacter pylori / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007407:Interviews as Topic; D016015:Logistic Models; D016393:Lymphoma, B-Cell / Q000175:diagnosis / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D012307:Risk Factors; D016036:Seroepidemiologic Studies; D013030:Spain / Q000453:epidemiology; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000382:microbiology*
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G