Establishment of Cre/LoxP recombination system in transgenic rats.
The rat has offered an important animal model in biomedical research including surgical procedure. However, advanced genetic manipulation has progressed less far in the rat than in the mouse. Here we report the Cre/LoxP transgenic rat system, demonstrating conditional chromosomal translocation both in the fertilization and adult stage, spatio-temporal gene controlling by catheter-based adenoviral gene transfer, and muscular fusion events in the limb transplant. Taking advantage of the larger body size of the rat than the mouse, this rat system provides a potential value to evaluate biomedical and therapeutic significance for gene therapy and regenerative medicine.
Sato
Yuki
Y
Division of Organ Replacement Research, Center for Molecular Medicine, Jichi Medical School, Tochigi 329-0498, Japan.
Endo
Hitoshi
H
Ajiki
Takashi
T
Hakamata
Yoji
Y
Okada
Takashi
T
Murakami
Takashi
T
Kobayashi
Eiji
E
D000818:Animals; D030801:Animals, Genetically Modified; D000831:Animals, Newborn; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology / Q000502:physiology; D010179:Pancreas / Q000166:cytology / Q000378:metabolism; D011401:Promoter Regions, Genetic; D051381:Rats; D017208:Rats, Wistar; D011995:Recombination, Genetic; D016378:Tissue Transplantation; D019076:Transgenes