title: Cloning and identification of NS5ATP2 gene and its spliced variant transactivated by hepatitis C virus non-structural protein 5A.
abstract: OBJECTIVE
  To clone, identify and study new NS5ATP2 gene and its spliced variant transactivated by hepatitis C virus non-structural protein 5A.
  
  
  METHODS
  On the basis of subtractive cDNA library of genes transactivated by NS5A protein of hepatitis C virus, the coding sequence of new gene and its spliced variant were obtained by bioinformatics method. Polymerase chain reaction (PCR) was conducted to amplify NS5ATP2 gene.
  
  
  RESULTS
  The coding sequence of a new gene and its spliced variant were cloned and identified successfully.
  
  
  CONCLUSIONS
  A new gene has been recognized as the new target transactivated by HCV NS5A protein. These results brought some new clues for studying the biological functions of new genes and pathogenesis of the viral proteins.
authors.affiliation: Department of Infectious Diseases, First Hospital of Xi'an Jiaotong University, Shaanxi Province, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Cheng
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Hong
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Wang
authors.forename: Shu-Lin
authors.initials: SL
authors.lastname: Zhang
mesh_terms: D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D001483:Base Sequence; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D015967:Gene Expression Regulation, Viral; D015723:Gene Library; D016174:Hepacivirus / Q000235:genetics*; D018197:Hepatoblastoma; D006801:Humans; D008113:Liver Neoplasms; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012326:RNA Splicing; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000378:metabolism; D012324:RNA-Dependent RNA Polymerase
chemical_list: D000952:Antigens, Polyomavirus Transforming; D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase