Cloning and functional analysis of the promoter of ZNF268 gene.
The human ZNF268 gene is a novel C2H2 zinc finger gene restrictively expressed in the liver of human embryo. As an initial study on the regulation of its expression, 2.5 kb fragment of the ZNF268 5'-flanking region was cloned from human genome by PCR. This fragment was inserted into pEGFP1 vector and then the recombinant plasmid was transfected into several cell lines by liposomal transfection method. EGFP expression was observed in four cell lines under confocal laser scanning microscope. A series of 5'-deletion fragments from -2456(-)+77 bp to -20(-)+77 bp were inserted into pCAT-Basic vector to construct recombinant reporter plasmids, which were then transfected into Hela cells for deletion analysis. Results of deletion analysis revealed that an important region for transcriptional activity lies between -156 and -20 bp.
Peng
Xiao
X
Department of Biotechnology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Sun
Yan
Y
Liu
Hui
H
Gou
De-Ming
DM
Li
Wen-Xin
WX
D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D005453:Fluorescence; D006801:Humans; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics*; D016385:TATA Box
D004268:DNA-Binding Proteins; D012097:Repressor Proteins; C482295:ZNF268 protein, human