title: Development of a two-site ELISA assay for the dimeric form of human TFF1.
abstract: TFF1 is one of three human trefoil proteins expressed principally in the gastrointestinal tract in normal tissues. TFF1 protects the gastric mucosa against damage as a result of its ability to facilitate reconstitution of damaged gastric mucosa and its involvement in the secretion and structure of gastric mucus. The most biologically active molecular form in cell culture and animal models tested is a dimer formed by a disulfide bond between two cysteine residues close to the C terminus of the protein. We have therefore developed an assay for this form of TFF1 which should facilitate its measurement in biological samples.
authors.affiliation: School of Clinical and Laboratory Sciences, University of Newcastle upon Tyne, Royal Victoria Infirmary, NE14LP, UK.
authors.forename: M
authors.initials: M
authors.lastname: Pu
authors.forename: F E B
authors.initials: FE
authors.lastname: May
authors.forename: R J
authors.initials: RJ
authors.lastname: Playford
authors.forename: M-H
authors.initials: MH
authors.lastname: Ruchaud-Sparagano
authors.forename: B R
authors.initials: BR
authors.lastname: Westley
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D019281:Dimerization; D004220:Disulfides / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D011506:Proteins / Q000032:analysis / Q000276:immunology*; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins
chemical_list: D000911:Antibodies, Monoclonal; D004220:Disulfides; D011506:Proteins; C051838:TFF1 protein, human; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins