Seed-specific promoters direct gene expression in non-seed tissue.
The organ specificity of four promoters that are known to direct seed-specific gene expression was tested. Whereas the phaseolin (phas)- and legumin B4 (leB4)-promoters were from genes encoding 7S and 11S globulins from Phaseolus vulgaris and Vicia faba, respectively, the usp- and the sbp-promoters were from non-storage protein genes of V. faba. The expression of different promoter-reporter gene fusions was followed either by RT-PCR or by registering the reporter enzyme activity in organs of transgenic tobacco, pea, narbon bean, or linseed. In addition to seeds, the promoters directed reporter gene expression in pollen and in seed coats. USP-, vicilin- and legumin-mRNA were detected by RT-PCR in pollen of Pisum sativum and V. faba. Expression during microsporogenesis and embryogenesis seems to be a general character of various seed protein genes.
Zakharov
Alexander
A
Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstr. 3, D-06466 Gatersleben, Germany.
Giersberg
Martin
M
Hosein
Felicia
F
Melzer
Michael
M
Müntz
Klaus
K
Saalbach
Isolde
I
D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D018532:Pisum sativum / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D018514:Plant Structures / Q000235:genetics; D030821:Plants, Genetically Modified; D011058:Pollen / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000235:genetics*; D000516:alpha-Amylases / Q000235:genetics; D000097793:Legumins
D010940:Plant Proteins; D012333:RNA, Messenger; D000516:alpha-Amylases; D000097793:Legumins; C048826:phaseolin protein, Phaseolus vulgaris