Vindoline synthesis in in vitro shoot cultures of Catharanthus roseus.
Vindoline, the major alkaloid in cultures of Catharanthus roseus shoots, reached 2 mg g(-1) dry wt after 27 d in culture. Maximal vindoline accumulation coincided with maximum activities of deacetoxyvindoline 4-hydroxylase, deacetylvindoline acetyl-CoA acetyl transferase and tryptophan decarboxylase. Shoot exposure to jasmonate shortened the time required for the maximal vindoline accumulation to 14 d.
Hernández-Domínguez
Elizabeta
E
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigacíon Cientifíca de Yucatán, Calle 43 No. 130 Chuburná 97200, Mérida Yucatán, México.
Campos-Tamayo
Freddy
F
Vázquez-Flota
Felipe
F
D000101:Acetyl-CoA C-Acetyltransferase / Q000378:metabolism; D000470:Alkaloids / Q000032:analysis; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000378:metabolism; D030008:Catharanthus / Q000378:metabolism*; D018929:Cell Culture Techniques; D003517:Cyclopentanes / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism; D054883:Oxylipins; D018520:Plant Shoots / Q000378:metabolism; D013997:Time Factors; D014747:Vinblastine / Q000031:analogs & derivatives* / Q000096:biosynthesis*
D000470:Alkaloids; D003517:Cyclopentanes; D054883:Oxylipins; C009667:vindoline; D014747:Vinblastine; C011006:jasmonic acid; D006899:Mixed Function Oxygenases; C052273:hyoscyamine (6S)-dioxygenase; D000101:Acetyl-CoA C-Acetyltransferase; D001142:Aromatic-L-Amino-Acid Decarboxylases