title: Lipids of the zygomycete Absidia corymbifera F-965.
abstract: The cell lipids of the zygomycete Absidia corymbifera F-965 extracted with isopropanol and CHCl3-MeOH mixtures at the exponential growth phase comprise 20+/-2% of mycelium dry wt. The lipids consist of: triacylglycerols (51% of the total lipids extracted), diacylglycerols (9%), monoacylglycerols (3%), ergosterol (5%), ergosterol peroxide (5alpha,8alpha-epidioxyergosta-6,22-diene-3beta-ol) (3%), fatty-acid esters of ergosterol (less than 0.5%), free fatty acids (4%), glucocerebroside (3%), and glycerophospholipids (22%). The main phospholipids are phosphatidylethanolamine (39% of the total phospholipids), phosphatidyl-myo-inositol (17%), diphosphatidylglycerol (12%), phosphatidic acid (7%), phosphatidylcholine (6%), phosphatidylglycerol (3%), and two unusual phospholipids reported earlier, N-acetylphosphatidylethanolamine (7%) and N-ethoxycarbonyl phosphatidylethanolamine (9%). In addition, two unknown acidic phospholipids are present in traces. Saturated fatty acids of the lipids are dominated by n-hexadecanoic acid and unsaturated ones by octadecenoic acid; octadecadienoic and octadecatrienoic acids are present in lesser amounts. Ergosterol peroxide as well as the above glucocerebroside which contains 9-methylsphinga-4(E),9(E)-dienine have first been revealed in zygomycetes.
authors.affiliation: Russian Research Centre Hydrobios, Ministry of Health, ul. Kosmonavtov 18, korp. 2, Moscow 129301, Russia.
authors.forename: Stanislav G
authors.initials: SG
authors.lastname: Batrakov
authors.forename: Inessa V
authors.initials: IV
authors.lastname: Konova
authors.forename: Vladimir I
authors.initials: VI
authors.lastname: Sheichenko
authors.forename: Stanislav E
authors.initials: SE
authors.lastname: Esipov
authors.forename: Lyudmila A
authors.initials: LA
authors.lastname: Galanina
authors.forename: Larisa N
authors.initials: LN
authors.lastname: Istratova
authors.forename: Yana E
authors.initials: YE
authors.lastname: Sergeeva
mesh_terms: D020095:Absidia / Q000737:chemistry* / Q000235:genetics; D002849:Chromatography, Gas; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D005963:Glucosylceramides / Q000737:chemistry / Q000302:isolation & purification; D008055:Lipids / Q000737:chemistry* / Q000302:isolation & purification; D015394:Molecular Structure; D010743:Phospholipids / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D005227:Fatty Acids; D005963:Glucosylceramides; D008055:Lipids; D010743:Phospholipids; C036071:ergosterol-5,8-peroxide; D004875:Ergosterol