title: [Experimental study on islet cells in rats under condition of three-dimensional microgravity].
abstract: OBJECTIVE
  To study the survival rate and secretory function of islet cells in rats under condition of three-dimensional microgravity.
  
  
  METHODS
  Isolated islet cells were assigned to flask-cultured or bioreactor-cultured. Survival rate of islets cultured for days 3, 7, 14 in stationary flasks or microgravity bioreactors was measured by AO-PI double-staining. Cultured islets were identified by dithizone (DTZ) staining, and insulin contents of different culture liquids were measured by radioimmunoassay.
  
  
  RESULTS
  Pancreatic islets stained nacarat with DTZ were easily visualized. When islet cells were cultured for 7 days and 14 days, survival rate of bioreactor-cultured islets was (0.9000 +/- 0.0107)% and 0.8038% +/- 0.0092% and higher than flask-cultured islets (P < 0.01). Insulin level of bioreactor-cultured islets is (70.875 +/- 0.31) m micro /L on the cultured 7 days while flask-cultured islets is (41.246 +/- 0.35) m micro /L. There was statistically significant difference of insulin production between the two groups (P < 0.01). Bioreactor-cultured islet contents were higher than flask-cultured on the cultured 14, 21 and 30 days (P < 0.01).
  
  
  CONCLUSIONS
  Islet cells survival rate and secretory function revealed that bioreactor-cultured islets functioned better compared with flask-cultured islets.
authors.affiliation: The Key Laboratory of Cell Transplantation of Ministry of Health, Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Song
authors.forename: Xiu-qing
authors.initials: XQ
authors.lastname: Duan
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xi
authors.initials: X
authors.lastname: Li
authors.forename: Li-ou
authors.initials: LO
authors.lastname: Han
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.forename: Chun-fang
authors.initials: CF
authors.lastname: Song
authors.forename: Lian-hong
authors.initials: LH
authors.lastname: Jin
mesh_terms: D000818:Animals; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival; D002478:Cells, Cultured; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018474:Weightlessness Simulation
chemical_list: D007328:Insulin