Expression pattern and raft association of NIPSNAP3 and NIPSNAP4, highly homologous proteins encoded by genes in close proximity to the ATP-binding cassette transporter A1.
The highly homologous genes NIPSNAP3 and NIPSNAP4, with 87% amino acid identity, are members of the NIPSNAP family with putative roles in vesicular trafficking. NIPSNAP3 mRNA and NIPSNAP4 mRNA and protein were detected in multiple tissues and cells at varying degrees. Interestingly, NIPSNAP3 is most highly expressed in skeletal muscle, where NIPSNAP4 has a low mRNA abundance. NIPSNAP4 was found associated with membranes and partly localized in rafts. The ubiquitous expression of the highly conserved NIPSNAPs and their association with membranes further support an important cellular function of these proteins probably linked to vesicular trafficking. The NIPSNAP3 and NIPSNAP4 genes are located in close proximity to the 3' end of the ATP-binding cassette transporter A1 (ABCA1), whose mutations cause familial high-density lipoprotein deficiency syndromes. The adjacent genomic location and the finding that ABCA1 is a regulator of vesicular trafficking may indicate a functional relation of these proteins, even though NIPSNAP4 does not interact directly with ABCA1 nor is its expression altered in cells with mutated ABCA1.
Buechler
Christa
C
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93042 Regensburg, Germany.
Bodzioch
Marek
M
Bared
Salim Maa
SM
Sigruener
Alexander
A
Boettcher
Alfred
A
Lapicka-Bodzioch
Katarzyna
K
Aslanidis
Charalampos
C
Duong
Chinh Quoc
CQ
Grandl
Margot
M
Langmann
Thomas
T
Dembinska-Kiec
Aldona
A
Schmitz
Gerd
G
D064286:ATP Binding Cassette Transporter 1; D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D018938:Caco-2 Cells; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics; D006224:Cricetinae; D003412:Cricetulus; D019073:HT29 Cells; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D021962:Membrane Microdomains / Q000378:metabolism*; D008969:Molecular Sequence Data; D011092:Polyethylene Glycols / Q000737:chemistry; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D013631:Tangier Disease / Q000235:genetics*; D022161:Transport Vesicles / Q000502:physiology; D033921:Vesicular Transport Proteins
C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters; D036341:Intercellular Signaling Peptides and Proteins; C113197:NIPSNAP1 protein, human; C495378:NIPSNAP3A protein, human; D011506:Proteins; C073119:TAP1 protein, human; D033921:Vesicular Transport Proteins; C017474:Lubrol WX; D011092:Polyethylene Glycols