title: Postweaning multisystemic wasting syndrome (PMWS) in the Philippines: porcine circovirus type 2 (PCV2) detection and characterization.
abstract: Four swine facilities located in Northern Luzon, in the Philippines, showed lesions and clinical signs similar to those described in post weaning multisystemic wasting syndrome (PMWS). Post-mortem and histopathological examination revealed the presence of typical lymphoid lesions caused by porcine circovirus type 2 (PCV2). An in situ hybridization (ISH) technique allowed the detection of PCV2 nucleic acid associated with the lesions in target tissues. PCV2 DNA from paraffin embedded tissues was extracted, purified and sequenced. The phylogenetic analysis of the sequences obtained confirmed their identity, and grouped them into a PCV2 subgroup, together with some Canadian, French and Dutch isolates. This report is the first description of the presence of PMWS in the Philippines.
authors.affiliation: Laboratorios Hipra, S.A., Girona, Spain.
authors.forename: Jaime
authors.initials: J
authors.lastname: Maldonado
authors.forename: Joaquim
authors.initials: J
authors.lastname: Ségales
authors.forename: Maria
authors.initials: M
authors.lastname: Calsamiglia
authors.forename: David
authors.initials: D
authors.lastname: Llopart
authors.forename: Marina
authors.initials: M
authors.lastname: Sibila
authors.forename: Zoilo
authors.initials: Z
authors.lastname: Lapus
authors.forename: Pere
authors.initials: P
authors.lastname: Riera
authors.forename: Carlos
authors.initials: C
authors.lastname: Artigas
mesh_terms: D000818:Animals; D001483:Base Sequence; D018173:Circoviridae Infections / Q000453:epidemiology / Q000662:veterinary*; D017925:Circovirus / Q000235:genetics*; D016000:Cluster Analysis; D017403:In Situ Hybridization; D008198:Lymph Nodes / Q000821:virology; D008969:Molecular Sequence Data; D010679:Philippines / Q000453:epidemiology; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D034421:Sus scrofa; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*; D019282:Wasting Syndrome / Q000453:epidemiology / Q000662:veterinary*