Smoking cessation rapidly increases circulating progenitor cells in peripheral blood in chronic smokers.
OBJECTIVE
Circulating endothelial progenitor cells (EPCs) contribute to postnatal angiogenesis. The number of circulating EPCs has an inverse correlation with coronary risk scores. However, the effect of smoking on the number of circulating EPCs is not well-known.


RESULTS
We examined the effects of chronic smoking and of smoking cessation on EPC levels. Circulating EPCs were quantified by flow cytometry as CD45lowCD34+CD133+ (progenitor cells [PCs]) or CD45lowCD34+CD133+VEGFR2+ (EPCs) in 14 nonsmokers and in 15 smokers. All smokers quit smoking. Eight quit smoking with nicotine patch and 7 without nicotine patch. PC/EPC levels were inversely correlated with the number of cigarettes smoked. Circulating PCs/EPCs increased rapidly after cessation (P<0.0001) and decreased again after resumption of smoking to the level similar to that before cessation (P=0.0031). The magnitude of increase in EPCs was greater in light smokers than in heavy smokers.


CONCLUSIONS
The number of circulating PCs/EPCs was reduced in chronic smokers. Smoking cessation led to a rapid restoration of PC/EPC levels. The recovery of EPC levels was greater in light smokers than in heavy smokers. The decreased number of circulating EPCs would make smokers susceptible to cardiovascular disease, and even short-time cessation of smoking may be an effective means to reduce cardiovascular risk.
Kondo
Takahisa
T
Department of Cardiology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan. takahisa@med.nagoya-u.ac.jp
Hayashi
Mutsuharu
M
Takeshita
Kyosuke
K
Numaguchi
Yasushi
Y
Kobayashi
Koichi
K
Iino
Shigeo
S
Inden
Yasuya
Y
Murohara
Toyoaki
T
D000071916:AC133 Antigen; D000328:Adult; D015703:Antigens, CD; D018952:Antigens, CD34 / Q000032:analysis; D001772:Blood Cell Count; D002318:Cardiovascular Diseases / Q000097:blood / Q000453:epidemiology / Q000209:etiology; D002784:Cholesterol / Q000097:blood; D005434:Flow Cytometry; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D007249:Inflammation / Q000097:blood; D017493:Leukocyte Common Antigens / Q000032:analysis; D007958:Leukocyte Count; D008297:Male; D059630:Mesenchymal Stem Cells; D010455:Peptides / Q000032:analysis; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects / Q000097:blood*; D016540:Smoking Cessation; D014280:Triglycerides / Q000097:blood
D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D014280:Triglycerides; D002784:Cholesterol; D017493:Leukocyte Common Antigens