title: A powerful antiradiation compound revealed by a new high-throughput screening method.
abstract: We present a new high-throughput screening method for the selection of powerful water-soluble antiradiation compounds. This method, which uses conventional immunoassay techniques, allowed the capacity of a given compound to protect thymidine from irradiation to be evaluated. By applying this assay to an antioxidant library, we showed for the first time that norbadione A, a well-known mushroom pigment, has pronounced atypical antiradiation properties.
authors.affiliation: Service de Marquage Moléculaire et de Chimie Bio-Organique, DBJC/DSV CEA Saclay, 91191 Gif sur Yvette Cedex, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Meunier
authors.forename: Marine
authors.initials: M
authors.lastname: Desage-El Murr
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Nowaczyk
authors.forename: Thierry
authors.initials: T
authors.lastname: Le Gall
authors.forename: Serge
authors.initials: S
authors.lastname: Pin
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Renault
authors.forename: Didier
authors.initials: D
authors.lastname: Boquet
authors.forename: Christophe
authors.initials: C
authors.lastname: Créminon
authors.forename: Eric
authors.initials: E
authors.lastname: Saint-Aman
authors.forename: Alain
authors.initials: A
authors.lastname: Valleix
authors.forename: Frédéric
authors.initials: F
authors.lastname: Taran
authors.forename: Charles
authors.initials: C
authors.lastname: Mioskowski
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D000363:Agaricales / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry / Q000145:classification / Q000494:pharmacology; D001685:Biological Factors / Q000737:chemistry / Q000494:pharmacology; D004247:DNA / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005069:Evaluation Studies as Topic; D007118:Immunoassay / Q000379:methods*; D010648:Phenylacetates / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry; D011837:Radiation-Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D012995:Solubility; D013936:Thymidine / Q000737:chemistry* / Q000528:radiation effects; D013997:Time Factors
chemical_list: D000975:Antioxidants; D001685:Biological Factors; D010648:Phenylacetates; D010936:Plant Extracts; D011837:Radiation-Protective Agents; D004247:DNA; C464564:norbadione A; D015107:4-Butyrolactone; D013936:Thymidine