Characterization of cholinesterase activity from different tissues of Nile tilapia (Oreochromis niloticus).
Cholinesterases (ChE) from brain, muscle and liver in Nile tilapia (Oreochromis niloticus) were characterized using three substrates: acetylthiocholine iodide, propionylthiocholine iodide, and butyrylthiocholine iodide. Eserine was used as a total ChE inhibitor; BW284c51 and iso-OMPA were used as selective inhibitors for acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE), respectively. The results indicate that AChE is the enzyme present in brain, whereas in both liver and muscle, the presence of atypical ChEs are suggested. These findings indicate that characterization of ChE is necessary prior to use in monitoring programs.
Rodríguez-Fuentes
Gabriela
G
Department of Marine Resources, Centro de Investigación y de Estudios Avanzados del I.P.N., Unidad Mérida, km 6 Antigua Carretera a Progreso, Cordemex, 97310 Mérida, Yucatán, México.
Gold-Bouchot
Gerardo
G
D000122:Acetylthiocholine / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D002093:Benzenaminium, 4,4'-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D002092:Butyrylthiocholine / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000378:metabolism*; D002802:Cholinesterases / Q000378:metabolism*; D023681:Cichlids / Q000378:metabolism*; D004784:Environmental Monitoring / Q000379:methods*; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D010830:Physostigmine / Q000378:metabolism; D012044:Regression Analysis; D013770:Tetraisopropylpyrophosphamide / Q000378:metabolism; D013860:Thiocholine / Q000031:analogs & derivatives* / Q000378:metabolism
D002800:Cholinesterase Inhibitors; C009063:propionylthiocholine; D002093:Benzenaminium, 4,4'-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide; D000122:Acetylthiocholine; D002092:Butyrylthiocholine; D013770:Tetraisopropylpyrophosphamide; D013860:Thiocholine; D010830:Physostigmine; D002802:Cholinesterases