Induction of partial protection in mice after oral administration of Lactococcus lactis producing Brucella abortus L7/L12 antigen.
The Brucella abortus ribosomal protein L7/L12 is an immunodominant antigen and an interesting candidate for the development of oral live vaccines against brucellosis. Here, a recombinant Lactococcus lactis strain producing L7/L12 under the control of nisin inducible promoter was orally administered to BALB/c mice. Significant levels of anti-L7/L12 specific IgA detected in feces revealed an induced local humoral immune response. However, serum analysis did not reveal any anti-L7/L12 antibodies suggesting the absence of a systemic response. Nevertheless, the vaccinated mice showed a partial protective immunity against B. abortus virulent strain (S2308) challenged by intraperitoneal inoculation.
Pontes
Daniela S
DS
Department of General Biology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Dorella
Fernanda A
FA
Ribeiro
Luciana A
LA
Miyoshi
Anderson
A
Le Loir
Yves
Y
Gruss
Alexandra
A
Oliveira
Sérgio C
SC
Langella
Philippe
P
Azevedo
Vasco
V
D000284:Administration, Oral; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D002004:Brucella Vaccine / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*; D002003:Brucella abortus / Q000235:genetics / Q000276:immunology*; D002006:Brucellosis / Q000276:immunology / Q000517:prevention & control; D004797:Enzyme-Linked Immunosorbent Assay; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012269:Ribosomal Proteins / Q000235:genetics / Q000276:immunology*
D000942:Antigens, Bacterial; D002004:Brucella Vaccine; D012269:Ribosomal Proteins; C016700:ribosomal protein L7-L12