Growth enhancement and food conversion efficiency of transgenic fish Labeo rohita.
Three family lines of fast growing transgenic rohu Labeo rohita (rohu) were generated by electroporated-sperm-mediated transfer of the vectors harboring CMV promoter or grass carp beta-actin promoter fused to endogenous rohu GH (rGH) cDNA. The gene transfer efficiency was 25%. The transgenic rohu (family line 1) with CMV promoter showed a growth enhancement of four times normal size, whereas those (family lines 2 and 3) generated with beta-actin promoter grew 4.5 and 5.8 times faster than their respective control siblings. Southern analysis confirmed the transgene extrachromosomal (Te) persistence until the 60th week in family 1. The individuals of family lines 2 and 3, however, showed integration (Ti), as well as persistence as extrachromosomal copies (Te) until the age of 30 weeks. Mosaicism of the transgene was shown at the levels of its presence and expression. The ectopic expression of rGH mRNA was confirmed by RT-PCR. Feeding experiments revealed that the transgenic rohu ate food at a lower rate but grew more efficiently than their control siblings.
Thayanithy
Venugopal
V
Department of Genetics, Center for Advanced Studies in Functional Genomics, School of Biological Sciences, Madurai Kamaraj University, Madurai 625 021 India.
Venugopal
Thayanithy
T
Anathy
Vikas
V
Kirankumar
Santhakumar
S
Pandian
Thavamani Jegajothivel
TJ
D000199:Actins / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified; D017756:Aquaculture; D015139:Blotting, Southern; D001837:Body Weights and Measures; D003530:Cyprinidae / Q000235:genetics / Q000254:growth & development*; D003587:Cytomegalovirus / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004435:Eating / Q000235:genetics / Q000502:physiology*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D007194:India; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D019076:Transgenes / Q000235:genetics
D000199:Actins; D018076:DNA, Complementary; D012333:RNA, Messenger