Biotinylated geldanamycin.
Inhibition of the 90 kDa heat shock proteins (Hsp90) represents a promising new chemotherapeutic approach for the treatment of several cancers. Hsp90 is essential to the survival of cancer cells and is inhibited by members of the ansamycin family of antibiotics. In particular, the quinone-containing antibiotics geldanamycin (GDA) and herbimycin A inhibit Hsp90 function in vitro at low micromolar concentrations via interaction with an ATP binding domain. Many proteins bind ATP, and the discovery of selective Hsp90 inhibitors requires the identification of other proteins that bind GDA and may cause undesired effects. Biotinylated analogues of GDA with varying tether lengths have been synthesized to elucidate other proteins that competitively bind GDA. Analogues containing a photolabile tether have also been prepared as a complementary method for the removal of GDA-bound proteins from neutravidin-containing resin. Preliminary studies indicate several proteins other than Hsp90 are isolated with biotinylated GDA.
Clevenger
Randell C
RC
Department of Medicinal Chemistry and The Center for Protein Structure and Function, The University of Kansas, 1251 Wescoe Hall Dr, Malott Hall 4070, Lawrence, Kansas 66045-7564, USA.
Raibel
Joseph M
JM
Peck
Angela M
AM
Blagg
Brian S J
BS
D000255:Adenosine Triphosphate / Q000378:metabolism; D016227:Benzoquinones; D019921:Biotinylation; D018841:HSP90 Heat-Shock Proteins / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D047029:Lactams, Macrocyclic; D010777:Photochemistry; D011485:Protein Binding; D011809:Quinones / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D017828:Rifabutin / Q000031:analogs & derivatives
D016227:Benzoquinones; D018841:HSP90 Heat-Shock Proteins; D047029:Lactams, Macrocyclic; D011809:Quinones; D017828:Rifabutin; C020754:herbimycin; D000255:Adenosine Triphosphate; C001277:geldanamycin