Novel methylcellulose-immobilized cation-exchange precolumn for on-line enrichment of cationic drugs in plasma.
We developed a novel methylcellulose-immobilized strong cation-exchange (MC-SCX) precolumn for direct analysis of drugs in plasma. MC-SCX consists of silica gel with a methylcellulose outer-surface and a 2-(4-sulfophenyl) ethyl phase inner-surface. The MC-SCX precolumn was evaluated by direct analysis using pyridoxine, atenolol and sulpiride spiked in plasma, using a column-switching HPLC system. Each drug was retained and enriched on MC-SCX using an acidic mobile phase, which resulted in good linearity, sufficient reproducibility, intra- and inter day precision, and accuracy in analytical ion-pair LC with trifluoroacetic acid. The analytical methods for model drugs were applied to pharmacokinetics of atenolol and sulpiride in rats.
Yamamoto
Eiichi
E
Analytical Research Laboratories, Eisai Co., Ltd., 5-1-3 Tokodai, Tsukuba, Ibaraki 300-2635, Japan. e-yamamoto@hhc.eisai.co.jp
Sakaguchi
Takahisa
T
Kajima
Takashi
T
Mano
Nariyasu
N
Asakawa
Naoki
N
D000818:Animals; D002411:Cation Exchange Resins; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000295:instrumentation*; D008297:Male; D004364:Pharmaceutical Preparations / Q000097:blood*; D010599:Pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013056:Spectrophotometry, Ultraviolet
D002411:Cation Exchange Resins; D004364:Pharmaceutical Preparations