Ubc9 is required for damage-tolerance and damage-induced interchromosomal homologous recombination in S. cerevisiae.
Ubc9 is an enzyme involved in the conjugation of small ubiquitin related modifier (SUMO) to target proteins. A Saccharomyces cerevisiae ubc9 temperature sensitive (ts) mutant showed higher sensitivity to various DNA damaging agents such as methylmethanesulfonate (MMS) and UV at a semi-permissive temperature than wild-type cells. The sensitivity of ubc9ts cells was not suppressed by the introduction of a mutated UBC9 gene, UBC9-C93S, whose product is unable to covalently bind to SUMO and consequently fails to conjugate SUMO to target proteins. Diploid ubc9ts cells were more sensitive to various DNA damaging agents than haploid ubc9ts cells suggesting the involvement of homologous recombination in the sensitivity of ubc9ts cells. The frequency of interchromosomal recombination between heteroalleles, his1-1/his1-7 loci, in wild-type cells was remarkably increased upon exposure to MMS or UV. Although the frequency of spontaneous interchromosomal recombination between the heteroalleles in ubc9ts cells was almost the same as that of wild-type cells, no induction of interchromosomal recombination was observed in ubc9ts cells upon exposure to MMS or UV.
Maeda
Daisuke
D
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba-ku, Sendai 980-8578, Japan.
Seki
Masayuki
M
Onoda
Fumitoshi
F
Branzei
Dana
D
Kawabe
Yoh-Ichi
Y
Enomoto
Takemi
T
D004249:DNA Damage; D017931:DNA Primers; D008741:Methyl Methanesulfonate / Q000633:toxicity; D009154:Mutation / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011003:Ploidies; D011995:Recombination, Genetic / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects*; D012441:Saccharomyces cerevisiae; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism*; D013696:Temperature; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics / Q000378:metabolism*; D014466:Ultraviolet Rays; D000098843:Ubiquitin-Conjugating Enzyme UBC9
D017931:DNA Primers; D025841:Small Ubiquitin-Related Modifier Proteins; D008741:Methyl Methanesulfonate; D044763:Ubiquitin-Conjugating Enzymes; D000098843:Ubiquitin-Conjugating Enzyme UBC9