EGb761 pretreatment reduces monoamine oxidase activity in mouse corpus striatum during 1-methyl-4-phenylpyridinium neurotoxicity.
EGb761 produces reversible inhibition of both monoamine oxidase (MAO) isoforms in the central nervous system. 1-Methyl-4-phenylpyridinium (MPP+) neurotoxicity is prevented by treatment with the MAO inhibitor pargyline. We investigated EGb761's effect on striatal MAO activity during MPP+ neurotoxicity. C-57 black mice were pretreated with EGb761 (10 mg/kg) daily for 17 days followed by administration of MPP+ (0.72 mg/kg). MPP+ enhanced striatal MAO (30%) activity at 6 h, and EGb761 prevented this effect. MAO-B activity in striatum was enhanced (70%) 6 h after MPP+ administration and was reduced to almost normal levels in EGb761 + MPP+ group compared to MPP+ group. Pretreatment with EGb761 partially prevented (32%) the striatal dopamine-depleting effect of MPP+ and prevented the reduction in striatal tyrosine hydroxylase activity (100%). Results suggest that EGb761 supplements may be effective in reducing MAO activity as well as enhancement in dopamine metabolism, thereby preventing MPP+-neurotoxicity.
Rojas
Patricia
P
Laboratory of Neurotoxicology, Instituto Nacional de Neurología y Neurocirugía, Manuel Velasco Suárez, México, DF, México. prcastane@hotmail.com
Rojas
Carolina
C
Ebadi
Manuchair
M
Montes
Sergio
S
Monroy-Noyola
Antonio
A
Serrano-García
Norma
N
D015655:1-Methyl-4-phenylpyridinium / Q000633:toxicity*; D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D004298:Dopamine / Q000378:metabolism; D020441:Ginkgo biloba; D007700:Kinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008995:Monoamine Oxidase / Q000378:metabolism*; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D013997:Time Factors; D000096983:Ginkgo Extract
D015655:1-Methyl-4-phenylpyridinium; D004298:Dopamine; D008995:Monoamine Oxidase; D008996:Monoamine Oxidase Inhibitors; D010936:Plant Extracts; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract