[The effects of two TCM prescriptions, Tonifying Kidney and Strengthening Vital Energy, on NF-kappaB activing in aged mouse T cells].
OBJECTIVE
The aim of the present study was to explore the immunologic mechanism of delaying senescence by Strengthening Vital Energy(SVE), Tonifying Kidney (TK) and combined TK and SVE.


METHODS
Mice of 20 months were used as senescence model. The effects of the prescriptions on anti-CD3 antibody-induced NF-kappaB activity and the expression of NF-kappaB in T cells from aged mice were analyzed by electrophoretic mobility shift assay (EMSA) and Western blot, respectively.


RESULTS
NF-kappaB activity in anti-CD3 antibody-induced T cells from the aged mice was lower than that from young ones. The three prescriptions raised the activity of NF-kappaB in the T cells from the aged mice to certain extent. Combined TK and SVE had no influence on p65 and p50 subunits expressions.


CONCLUSIONS
The increased NF-kappaB activity may be one of mechanisms underlying the improvement of immune response in aged mice by Chinese medicines.
Song
Shu-xia
SX
Department of Experimental Zoology, Hebei Medical University, Shijiazhuang 050017, China. prosongsx@yahoo.com
Feng
Xu
X
Cai
Yue-hua
YH
Lü
Zhan-jun
ZJ
D000375:Aging / Q000187:drug effects* / Q000473:pathology; D000818:Animals; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism*; D052002:NF-kappa B p50 Subunit / Q000096:biosynthesis; D010946:Plants, Medicinal / Q000737:chemistry; D013601:T-Lymphocytes / Q000378:metabolism*; D051996:Transcription Factor RelA / Q000096:biosynthesis
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; D051996:Transcription Factor RelA