Interaction of oxoferrylmyoglobin and dinitrosyl-iron complexes.
It is shown that dinitrosyl-iron complexes (DNIC) with glutathione can reduce oxoferrylmyoglobin forming on interaction of tert-butyl hydroperoxide and metmyoglobin. A rapid decrease in the DNIC concentration was observed under the conditions of production of tert-butyl free radicals; however, destruction of DNIC in the presence of oxoferrylmyoglobin alone was negligible. It is demonstrated that DNIC reduces oxoferrylmyoglobin more than an order more efficiently than S-nitrosoglutathione and glutathione. DNIC also inhibits formation of the thiyl radicals of glutathione in a medium containing metmyoglobin and tert-butyl hydroperoxide. A mechanism of the antioxidant action of DNIC based on regeneration of the nitrosyl complexes from the products of their interaction with oxoferrylheme is proposed.
Shumaev
K B
KB
Russian Cardiology Research Center, Moscow, 121552, Russia. shumaev@vipmail.ru
Petrova
N E
NE
Zabbarova
I V
IV
Vanin
A F
AF
Topunov
A F
AF
Lankin
V Z
VZ
Ruuge
E K
EK
D000975:Antioxidants / Q000378:metabolism; D003497:Cyclic N-Oxides / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D008786:Metmyoglobin / Q000378:metabolism*; D009569:Nitric Oxide; D009589:Nitrogen Oxides / Q000378:metabolism*; D026422:S-Nitrosoglutathione / Q000378:metabolism; D020122:tert-Butylhydroperoxide / Q000378:metabolism
D000975:Antioxidants; D003497:Cyclic N-Oxides; D005609:Free Radicals; D009589:Nitrogen Oxides; C028048:ferrylmyoglobin; D008786:Metmyoglobin; C098505:5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide; D009569:Nitric Oxide; D026422:S-Nitrosoglutathione; C073661:dinitrosyl iron complex; D020122:tert-Butylhydroperoxide; D007501:Iron; D005978:Glutathione