title: Saccharomyces bulderi sp. nov., a yeast that ferments gluconolactone.
abstract: An unknown yeast species was isolated from maize silage and was determined to be novel on the basis of morphological and physiological characteristics, nucleotide sequence of domain D1/D2 of LSU rDNA and from its electrophoretic karyotype. The name for the proposed new species is Saccharomyces bulderi Middelhoven, Kurtzman et Vaughan-Martini (type strain CBS 8638, NRRL Y-27203, DBVPG 7127). S. bulderi is closely related to S. barnettii and S. exiguus from which it can be distinguished by having a double vitamin requirement of biotin and thiamine and by no or slow aerobic growth on raffinose, a sugar that on the contrary is fermented rapidly. Gluconolactone is rapidly fermented with ethanol, glycerol and carbon dioxide being the main products.
authors.affiliation: Laboratorium voor Microbiologie, Wageningen Agricultural University, P.O. Box 8033, 6700 EJ Wageningen, The Netherlands. wout.middelhoven@algemeen.micr.wau.nl
authors.forename: W J
authors.initials: WJ
authors.lastname: Middelhoven
authors.forename: C P
authors.initials: CP
authors.lastname: Kurtzman
authors.forename: A
authors.initials: A
authors.lastname: Vaughan-Martini
mesh_terms: D000332:Aerobiosis; D001710:Biotin / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D004271:DNA, Fungal / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005285:Fermentation; D020459:Genes, rRNA; D005942:Gluconates / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D007621:Karyotyping; D007783:Lactones; D000432:Methanol / Q000378:metabolism; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D012335:RNA, Ribosomal / Q000235:genetics; D011887:Raffinose / Q000378:metabolism; D012440:Saccharomyces / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013831:Thiamine / Q000378:metabolism; D003313:Zea mays / Q000382:microbiology
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D005942:Gluconates; D007783:Lactones; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S; D002245:Carbon Dioxide; D001710:Biotin; D011887:Raffinose; D005990:Glycerol; C010730:beta-glucono-1,5-lactone; D013831:Thiamine; D000432:Methanol