[The effects of M-CSF and IL-10 on IL-12 and IL-18 production and expressions of HLA-DR and CD80 by human monocytes].
OBJECTIVE
To explore the effects of M-CSF and IL-10 on IL-12 and IL-18 production and expressions of HLA-DR and CD80 by human peripheral blood monocytes.


METHODS
Human monocytes were isolated from blood of healthy donors, and cultured with M-CSF and or IL-10 alone or together. Then the monocytes and culture supernatant were collected respectively. IL-12 p40 and IL-18 levels in culture supernatant were detected by ELISA and the expressions of CD80 and HLA-DR on the monocytes were analyzed by FACS.


RESULTS
(1)M-CSF induced secretion of IL-18 by the monocytes, while IL-10 inhibited the production of IL-18 and antagonized enhancement of LPS-induced IL-18 production by M-CSF. Both M-CSF and IL-10 inhibited secretion of IL-12 p40 by the monocytes and had a synegistic effect in the inhibition. (2)M-CSF could induce while IL-10 inhibited HLA-DR expression. Moreover, IL-10 had an antagonistic action on HLA-DR expression induced by M-CSF. M-CSF had no influence on CD80 expression, while IL-10 induced CD80 expression.


CONCLUSIONS
M-CSF and IL-10 can regulate IL-12 and IL-18 production and HLA-DR and CD80 expressions by human monocytes, which may affect the activation and differentiation of T cells and T cell-mediated immune response.
Yao
Ting
T
Department of Pathogenic Biology and Immunology, Nanjing Medical Uiniversity, Nanjing 210029, China. immune@njmu.edu.cn
Wang
Shu-kui
SK
Chen
Jun
J
Yao
Kun
K
Qin
Jun-chuan
JC
Ji
Xiao-hui
XH
D018122:B7-1 Antigen / Q000096:biosynthesis*; D006684:HLA-DR Antigens / Q000096:biosynthesis*; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology*; D018664:Interleukin-12 / Q000096:biosynthesis*; D020382:Interleukin-18 / Q000096:biosynthesis*; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology
D018122:B7-1 Antigen; D006684:HLA-DR Antigens; D020382:Interleukin-18; D016753:Interleukin-10; D018664:Interleukin-12; D016173:Macrophage Colony-Stimulating Factor