title: Cloning of murine IL-22 receptor alpha 2 and comparison with its human counterpart.
abstract: We have identified the mouse and rat homologs of human interleukin-22 receptor alpha 2 (IL-22R alpha 2) and compared the localization, structure, and expression of the encoding murine and human genes. The mouse IL-22R alpha 2-encoding gene is located on chromosome 10A3 between, like in human, the genes for interferon-gamma R1 and IL-20R1. It spans a region of approximately 10 kb therefore being three times shorter than the human gene. Although the overall gene structure in both species is similar, the mouse gene lacks a counterpart to the third coding exon of the human gene known to be alternatively spliced. Like in human, mouse and rat IL-22R alpha 2 exist only as soluble receptors as deduced from the lack of transmembrane and intracellular domains encoding sequences. Quantitative expression analyses showed, analogically to the human system, a limited tissue distribution of mouse IL-22R alpha 2 mRNA. Differential modulation of IL-22R alpha 2 mRNA expression was observed upon systemic inflammation in mice in spleen, thymus, and lymph node.
authors.affiliation: Enabling Technologies, Schering AG, Berlin, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Weiss
authors.forename: K
authors.initials: K
authors.lastname: Wolk
authors.forename: B H
authors.initials: BH
authors.lastname: Grünberg
authors.forename: H-D
authors.initials: HD
authors.lastname: Volk
authors.forename: W
authors.initials: W
authors.lastname: Sterry
authors.forename: K
authors.initials: K
authors.lastname: Asadullah
authors.forename: R
authors.initials: R
authors.lastname: Sabat
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics; D003001:Cloning, Molecular; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000235:genetics; D018123:Receptors, Interleukin / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: C433253:IL22RA2 protein, human; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D018123:Receptors, Interleukin; C415369:interleukin-22 receptor