Determination of rofecoxib in human plasma and breast milk by high-performance liquid chromatographic assay.
A rapid and simple HPLC assay was developed for the determination of rofecoxib in human plasma and breast milk. After solid-phase extraction, rofecoxib was resolved on a C18 column and detected by UV detection at 272 nm. Standard curves were linear over the concentration range 10-2000 microg/L (r2 >0.99). Intra- and inter-day coefficients of variation for both matrices were <10% and the limit of quantification was around 10 microg/L.
Zhang
Mei
M
Department of Clinical Pharmacology, Christchurch School of Medicine and Health Sciences, University of Otago, P.O. Box 4345, Christchurch, New Zealand. mei.zhang@cdhb.govt.nz
Moore
Grant A
GA
Gardiner
Sharon J
SJ
Begg
Evan J
EJ
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016861:Cyclooxygenase Inhibitors / Q000032:analysis* / Q000097:blood; D006801:Humans; D007783:Lactones / Q000032:analysis* / Q000097:blood; D008895:Milk, Human / Q000737:chemistry*; D015203:Reproducibility of Results; D013450:Sulfones / Q000032:analysis* / Q000097:blood
D016861:Cyclooxygenase Inhibitors; D007783:Lactones; D013450:Sulfones; C116926:rofecoxib