title: Characterization of soluble non-covalent complexes between bovine serum albumin and beta-1,2,3,4,6-penta-O-galloyl-D-glucopyranose by MALDI-TOF MS.
abstract: Beta-1,2,3,4,6-penta-O-galloyl-D-glucopyranose (PGG) and soluble complexes of PGG with bovine serum albumin (BSA) were characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). PGG was also characterized by electrospray ionization mass spectrometry (ESI-MS). Similar fragmentation patterns of PGG were found in ESI-MS and MALDI-TOF MS. The apparent stoichiometries of non-covalent BSA-PGG complexes were determined by MALDI-TOF MS.
authors.affiliation: Department of Chemistry and Biochemistry, Miami University, Oxford, Ohio 45056, USA.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Chen
authors.forename: Ann E
authors.initials: AE
authors.lastname: Hagerman
mesh_terms: D047348:Hydrolyzable Tannins / Q000031:analogs & derivatives* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D010446:Peptide Fragments / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012995:Solubility; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013634:Tannins / Q000737:chemistry*
chemical_list: D047348:Hydrolyzable Tannins; D010446:Peptide Fragments; D013634:Tannins; C075490:serum albumin, bovine (152-168); D012710:Serum Albumin, Bovine; C435084:pentagalloylglucose