title: Molecular evidence of speciation between island and continental populations of Anopheles (Cellia) sundaicus (Diptera: Culicidae), a principal malaria vector taxon in Southeast Asia.
abstract: Anopheles sundaicus s.l. is a principal malaria vector taxon on islands and along the coastal areas of Southeast Asia. It has a wide geographical distribution and exhibits a high level of ecological and behavioral variability. Study of this taxon is crucial for understanding its biology and implementing effectise vector control measures. We compared populations of An. sundaicus from Vietnam, Thailand, and Malaysian Borneo by using two mitochondrial DNA markers: cytochrome oxidase I and cytochrome b. Genetic divergence, geographic separation, and cladistic analysis of relationships revealed the presence of two cryptic species: Anopheles sundaicus s.s. on Malaysian Borneo and An. sundaicus species A in coastal areas of Thailand and Vietnam. A polymerase chain reaction (PCR) assay was developed to easily identify these two species throughout their geographic distributions. The assay was based on sequence characterized amplified region derived from random amplified polymorphic DNA. This PCR identification method needs to be validated and adapted for the recognition of other possible species in the Sundaicus Complex.
authors.affiliation: Institute of Research for Development, Center of Biology and Management of Populations, Montferrier sur Lez, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Dusfour
authors.forename: Yvonne-Marie
authors.initials: YM
authors.lastname: Linton
authors.forename: Anna
authors.initials: A
authors.lastname: Cohuet
authors.forename: Ralph E
authors.initials: RE
authors.lastname: Harbach
authors.forename: Visut
authors.initials: V
authors.lastname: Baimai
authors.forename: Ho D
authors.initials: HD
authors.lastname: Trung
authors.forename: Chang Moh
authors.initials: CM
authors.lastname: Seng
authors.forename: Asmad
authors.initials: A
authors.lastname: Matusop
authors.forename: Sylvie
authors.initials: S
authors.lastname: Manguin
mesh_terms: D000818:Animals; D000852:Anopheles / Q000145:classification* / Q000235:genetics*; D001210:Asia, Southeastern; D004272:DNA, Mitochondrial / Q000235:genetics*; D003710:Demography; D003576:Electron Transport Complex IV; D005819:Genetic Markers; D005843:Geography; D006801:Humans; D007303:Insect Vectors; D008288:Malaria / Q000635:transmission*; D010802:Phylogeny
chemical_list: D004272:DNA, Mitochondrial; D005819:Genetic Markers; D003576:Electron Transport Complex IV