title: [An improved procedure for autologous gene-modified cancer vaccine preparation for active specific immunotherapy of disseminated solid tumors].
abstract: Resected material was used from 108 patients with disseminated skin melanoma and 47 patients suffering metastasized renal tumors to test procedures of tumor cell culture preparation and to search the best parameters. Gene tag 7 transfer, liposome delivery and electroporation were employed to stimulate immunogenic tumor cells. The transfer results were evaluated by expression of beta-galactosidase and EGFP genes whose products were detected microscopically. Transfer efficiency was boosted by 30% due to selecting suitable parameters of tumor cell modification. Maximum effectiveness was attained by individualized choice of the parameters. Yet, undoubtedly, the best way of cell isolation was mechanical fragmentation of tumor. To speed up cell production, DMEM/F12 medium should be recommended. It should contain cattle embryonic serum (20%), conditioned medium of cultured fibroblasts of human embryonic lungs (20%), transferin, insulin and selenium (standard dose).
authors.affiliation: N.N. Petrov Research Institute of Oncology, Ministry of Health of the RF, St. Petersburg.
authors.forename: A O
authors.initials: AO
authors.lastname: Danilov
authors.forename: S S
authors.initials: SS
authors.lastname: Larin
authors.forename: A B
authors.initials: AB
authors.lastname: Danilova
authors.forename: V M
authors.initials: VM
authors.lastname: Moiseenko
authors.forename: I A
authors.initials: IA
authors.lastname: Baldueva
authors.forename: S L
authors.initials: SL
authors.lastname: Kiselev
authors.forename: E A
authors.initials: EA
authors.lastname: Turkevich
authors.forename: A S
authors.initials: AS
authors.lastname: Barchuk
authors.forename: V V
authors.initials: VV
authors.lastname: Anisimov
authors.forename: G I
authors.initials: GI
authors.lastname: Gafton
authors.forename: V A
authors.initials: VA
authors.lastname: Kochnev
authors.forename: K P
authors.initials: KP
authors.lastname: Khanson
mesh_terms: D019496:Cancer Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000494:pharmacology; D018274:Electroporation; D006801:Humans; D016233:Immunotherapy, Active / Q000379:methods*; D007680:Kidney Neoplasms / Q000276:immunology / Q000628:therapy; D008081:Liposomes; D008545:Melanoma / Q000276:immunology / Q000628:therapy; D009369:Neoplasms / Q000276:immunology* / Q000473:pathology / Q000628:therapy*; D012878:Skin Neoplasms / Q000276:immunology / Q000628:therapy
chemical_list: D019496:Cancer Vaccines; D008081:Liposomes