[Experimental research on tea saponin ameliorating nonoxynol spermicidal action in vitro].
OBJECTIVE
To study the effect of tea saponin in ameliorating nonoxynol(N-9) spermicidal action in vitro.


METHODS
According to the improved spermicidal test method in vitro recommended by International Planned Parenthood Foundation (IPPF), we evaluated the minimum spermicidal concentration of N-9, tea saponin and their mixed solution in 20 s and 3 min.


RESULTS
The minimum spermicidal concentration of N-9 in the mixed solution was (0.13 +/- 0.05) g/L in 20 s and (0.05 +/- 0.004) g/L in 3 min, and that of the tea saponin in the mixed solution was (2.40 +/- 1.07) g/L in 20 s and (1.27 +/- 0.38) g/L in 3 min, compared with the single ingredient N-9 [(0.48 +/- 0.15) g/L in 20 s, (0.34 +/- 0.079 g/L in 3 min], and tea saponin [(5.78 +/- 1.40) g/L in 20 s, (1.71 +/- 0.176) g/L in 3 min], P < 0.01.


CONCLUSIONS
Tea saponin can improve N-9 spermicidal action in vitro, and tea saponin and nonoxynol have proved of synergic effect.
Wang
Zhengyi
Z
United Postdoctoral Workshop, Traditional Chinese Medical University & Enwei Corporation, Chengdu, Sichuan 610041, China. wzyy10@vip.sina.com
Zheng
Youming
Y
Guo
Kai
K
D000818:Animals; D004357:Drug Synergism; D008297:Male; D017137:Nonoxynol / Q000494:pharmacology*; D011817:Rabbits; D012503:Saponins / Q000494:pharmacology*; D013089:Spermatocidal Agents / Q000494:pharmacology*; D013662:Tea
D012503:Saponins; D013089:Spermatocidal Agents; D013662:Tea; D017137:Nonoxynol