Generation of anti-beta-amyloid antibodies via phage display technology.
The EFRH sequence, found to be the main anti-aggregating epitope corresponding to amino acids 3-6 of beta-amyloid peptide (AbetaP), was displayed on a phage and used as an antigen for immunization of mice, guinea pigs and rabbits. The generated antibodies recognize the full-length AbetaP (1-40) and exhibit similar biological properties to antibodies raised against whole soluble peptide and/or fibrillar beta-amyloid. EFRH-phage immunization of a transgenic mouse model of Alzheimer's disease evokes antibodies able to dissolve already formed beta-amyloid plaques, suggesting that they could become a therapeutic approach in treatment of the disease.
Frenkel
Dan
D
Department of Molecular Microbiology & Biotechnology, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Ramat Aviv, Tel-Aviv 69978, Israel.
Dori
Mally
M
Solomon
Beka
B
D000544:Alzheimer Disease / Q000378:metabolism / Q000628:therapy; D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D001435:Bacteriophages / Q000235:genetics; D000939:Epitopes / Q000008:administration & dosage* / Q000737:chemistry; D019151:Peptide Library
D016229:Amyloid beta-Peptides; D000906:Antibodies; D000939:Epitopes; D019151:Peptide Library