Porcine endogenous retroviral nucleic acid in peripheral tissues is associated with migration of porcine cells post islet transplant.
Porcine islets represent an alternative source of insulin-producing tissue, however, porcine endogenous retrovirus (PERV) remains a concern. In this study, SCID mice were transplanted with nonencapsulated (non-EC), microencapsulated (EC) or macroencapsulated (in a TheraCyte trade mark device) neonatal porcine islets (NPIs), and peripheral tissues were screened for presence of viral DNA and mRNA. To understand the role of an intact immune system in PERV incidence, mice with established NPI grafts were reconstituted with splenocytes. Peripheral tissues were screened for PERV and porcine DNA using PCR. Tissues with positive DNA were analyzed for PERV mRNA using RT-PCR. No significant difference was observed between non-EC and EC transplants regarding presence of PERV or porcine-specific DNA or mRNA. In reconstituted animals, little PERV or porcine DNA, and no PERV mRNA was detected. No PERV or porcine-specific DNA was observed in animals implanted with a TheraCyte trade mark device. In conclusion, an intact immune system significantly lowered the presence of PERV. Microencapsulation of islets did not alter PERV presence, however, macroencapsulation in the TheraCyte device did. Lower PERV incidence coincided with lower porcine DNA in peripheral tissues, linking the presence of PERV to migration of porcine cells.
Binette
Tanya M
TM
Surgical-Medical Research Institute, University of Alberta, Edmonton, Alberta, Canada.
Seeberger
Karen L
KL
Lyon
James G
JG
Rajotte
Ray V
RV
Korbutt
Gregory S
GS
D000818:Animals; D000831:Animals, Newborn; D002465:Cell Movement; D051546:Cyclooxygenase 2; D004279:DNA, Viral / Q000235:genetics; D003921:Diabetes Mellitus, Experimental; D004339:Drug Compounding; D020077:Endogenous Retroviruses / Q000235:genetics / Q000302:isolation & purification*; D007515:Islets of Langerhans / Q000166:cytology; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D007527:Isoenzymes / Q000235:genetics; D051379:Mice; D016513:Mice, SCID; D016133:Polymerase Chain Reaction; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics*; D012192:Retroviridae Infections / Q000175:diagnosis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000166:cytology; D013552:Swine; D013997:Time Factors; D014183:Transplantation, Heterologous
D004279:DNA, Viral; D007527:Isoenzymes; D012333:RNA, Messenger; D012367:RNA, Viral; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases