Control of mitotic entry after DNA damage in Drosophila.
In the presence of DNA damage, cells delay the entry into mitosis, presumably to allow time for repair. Methods to detect the delay of mitosis in a multicellular model organism, Drosophila melanogaster, are described here. These include the collection of embryos and larvae, irradiation with x-rays to damage DNA, and fixing and staining of tissues with an antibody to phosphorylated histone H3 to measure the mitotic index. These methods should be useful in identifying potential mutants that are unable to regulate mitosis following DNA damage.
Jaklevic
Burnley
B
Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, USA.
Purdy
Amanda
A
Su
Tin Tin
TT
D000818:Animals; D002453:Cell Cycle; D004249:DNA Damage; D004331:Drosophila melanogaster / Q000502:physiology*; D006657:Histones / Q000378:metabolism; D008938:Mitosis; D008953:Models, Anatomic; D009154:Mutation; D010766:Phosphorylation; D013696:Temperature; D013997:Time Factors; D014965:X-Rays
D006657:Histones