Suppression of neutrophil recruitment in mice by geranium essential oil.
BACKGROUND
In aromatherapy, essential oils are used as anti-inflammatory remedies, but experimental studies on their action mechanisms are very limited.


OBJECTIVE
To assess their anti-inflammatory activities, the effects of essential oils on neutrophil recruitment in mice were examined in vivo.


METHODS
The effect of essential oils on leukocyte and neutrophil recruitment induced 6 h after intraperitoneal injection of casein in mice was examined.


RESULTS
Leukocyte recruitment into the peritoneal cavity in mice was suppressed by intraperitoneal injections of geranium, lemongrass and spearmint oils at the dose of 5 microl/mouse, but was not by tea tree oil. This recruitment was inhibited dose-dependently by geranium oil. The suppression of leukocyte recruitment resulted from inhibition of neutrophil accumulation.


CONCLUSIONS
Some essential oils used as anti-inflammatory remedies suppress neutrophil recruitment into the peritoneal cavity in mice.
Abe
Shigeru
S
Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, Japan. sabe@main.teikyo-u.ac.jp
Maruyama
Naho
N
Hayama
Kazumi
K
Inouye
Shigeharu
S
Oshima
Haruyuki
H
Yamaguchi
Hideyo
H
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D031315:Geranium / Q000737:chemistry*; D007274:Injections, Intraperitoneal; D031337:Mentha spicata / Q000737:chemistry; D051379:Mice; D008813:Mice, Inbred ICR; D020556:Neutrophil Infiltration / Q000187:drug effects*; D009822:Oils, Volatile / Q000008:administration & dosage / Q000494:pharmacology*; D010529:Peritoneal Cavity / Q000166:cytology; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology*; D013729:Terpenes / Q000008:administration & dosage / Q000494:pharmacology
D000893:Anti-Inflammatory Agents; D009822:Oils, Volatile; D010938:Plant Oils; D013729:Terpenes; C052901:lemongrass oil