title: Small-interfering-RNA expression in cells based on an efficiently constructed dumbbell-shaped DNA.
authors.affiliation: Gene Function Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Central 4, 1-1-1 Higashi, Tsukuba Science City 305-8562, Japan.
authors.forename: Masumi
authors.initials: M
authors.lastname: Taki
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kato
authors.forename: Makoto
authors.initials: M
authors.lastname: Miyagishi
authors.forename: Yasuomi
authors.initials: Y
authors.lastname: Takagi
authors.forename: Kazunari
authors.initials: K
authors.lastname: Taira
mesh_terms: D004247:DNA / Q000138:chemical synthesis* / Q000378:metabolism; D011088:DNA Ligases / Q000737:chemistry; D017931:DNA Primers; D015316:Genetic Therapy; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000737:chemistry; D034741:RNA, Small Interfering / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D034741:RNA, Small Interfering; D049452:Green Fluorescent Proteins; D004247:DNA; D011088:DNA Ligases