title: Double chip protein arrays using recombinant single-chain Fv antibody fragments.
abstract: Protein arrays permit the parallel analysis of many different markers in a small sample volume. However, the problem of cross-reactivity limits the degree of multiplexing in parallel sandwich immunoassays (using monoclonal antibodies (mAbs)), meaning antibodies must be prescreened in order to reduce false positives. In contrast, we use a second chip surface for the local application of detection antibodies, thereby efficiently eliminating antibody cross-reactions. Here, we illustrate the potential advantages of using single-chain Fv fragments rather than mAbs as capture and detection molecules with this double chip technology.
authors.affiliation: nanotype, Graefelfing, Germany.
authors.forename: Ilka
authors.initials: I
authors.lastname: Gilbert
authors.forename: Susanne
authors.initials: S
authors.lastname: Schiffmann
authors.forename: Susanne
authors.initials: S
authors.lastname: Rubenwolf
authors.forename: Kristian
authors.initials: K
authors.lastname: Jensen
authors.forename: Thao
authors.initials: T
authors.lastname: Mai
authors.forename: Christian
authors.initials: C
authors.lastname: Albrecht
authors.forename: Andreas
authors.initials: A
authors.lastname: Lankenau
authors.forename: Gerald
authors.initials: G
authors.lastname: Beste
authors.forename: Kerstin
authors.initials: K
authors.lastname: Blank
authors.forename: Hermann E
authors.initials: HE
authors.lastname: Gaub
authors.forename: Hauke
authors.initials: H
authors.lastname: Clausen-Schaumann
mesh_terms: D019921:Biotinylation; D007128:Immunoglobulin Fragments / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000235:genetics* / Q000276:immunology; D040081:Protein Array Analysis / Q000379:methods*; D011994:Recombinant Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D012680:Sensitivity and Specificity
chemical_list: D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D011994:Recombinant Proteins; C055966:immunoglobulin Fv