title: Protection against ischemia/reperfusion injury by the cavitary two-layer method in canine small intestinal transplantation with reduction of reactive oxygen species.
abstract: BACKGROUND
  Ischemia and reperfusion (I/R) injury is a major determinant of early graft dysfunction and long-term graft survival in small intestinal transplantation. The cavitary two-layer method (TLM) has been reported to be superior to the University of Wisconsin cold storage method (UWM) in long-term preservation of canine small intestine. This study was designed to evaluate the protective effect of the cavitary TLM against I/R injury in canine small intestinal transplantation.
  
  
  METHODS
  Intestinal grafts harvested from beagles were allotransplanted after 24-hour preservation by UWM (group 1) or the cavitary TLM (group 2). The graft in the controls (group 3) was immediately allotransplanted without preservation. I/R injury was assessed by functional success rates, biochemical assay, graft adenosine triphosphate (ATP) and lipid peroxidation (LPO) concentrations, and histopathologic examination including TUNEL staining for apoptosis.
  
  
  RESULTS
  In group 1, ATP recovery was delayed after reperfusion, and most recipients died with hemorrhage of the grafts and lungs. In group 2, graft ATP concentrations recovered rapidly, and I/R injury was prevented with reduced LPO production, resulting in good outcome.
  
  
  CONCLUSIONS
  The cavitary TLM protected intestinal grafts against I/R injury evidenced by maintenance of graft ATP levels and reduction of LPO production compared with UWM in canine small intestinal transplantation.
authors.affiliation: Division of Gastroenterological Surgery, Department of Clinical Molecular Medicine, Kobe University Graduate School of Medicine, Japan.
authors.forename: Keitaro
authors.initials: K
authors.lastname: Kakinoki
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Fujino
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Shiri
authors.initials: S
authors.lastname: Li
authors.forename: Takuro
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Tanioka
authors.forename: Yonson
authors.initials: Y
authors.lastname: Ku
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kuroda
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D004285:Dogs; D005260:Female; D006085:Graft Survival; D020287:In Situ Nick-End Labeling; D007421:Intestine, Small / Q000098:blood supply* / Q000473:pathology / Q000503:physiopathology / Q000637:transplantation*; D015227:Lipid Peroxidation; D008297:Male; D009026:Mortality; D016377:Organ Transplantation / Q000379:methods*; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors*; D015427:Reperfusion Injury / Q000517:prevention & control*; D016019:Survival Analysis
chemical_list: D017382:Reactive Oxygen Species; D000255:Adenosine Triphosphate