title: Lentiviral-mediated gene transfer to model triplet repeat disorders.
abstract: This chapter describes the potential use of viral-mediated gene transfer in the central nervous system as a new strategy in developing animal models of neurodegenerative diseases. To illustrate the approach, procedures for the production of lentiviral vectors encoding polyQ proteins are provided, as well as methods for the determination of viral titers, in vitro infection, and basic protocols for in vivo studies in rodents.
authors.affiliation: Institute of Neuroscience, School of Life Sciences, Swiss Federal Institute of Technology Lausanne, Switzerland.
authors.forename: Etienne
authors.initials: E
authors.lastname: Régulier
authors.forename: Diana
authors.initials: D
authors.lastname: Zala
authors.forename: Patrick
authors.initials: P
authors.lastname: Aebischer
authors.forename: Nicole
authors.initials: N
authors.lastname: Déglon
mesh_terms: D000818:Animals; D002460:Cell Line; D005260:Female; D018014:Gene Transfer Techniques; D030342:Genetic Diseases, Inborn / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D016086:Lentivirus / Q000235:genetics*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D018911:Trinucleotide Repeats / Q000235:genetics*