Isolation of photosystem II reaction center complexes from plants.
Methods to isolate and purify 6- and 5-chlorophyll (Chl) D1/D2/Cyt b559 photosystem (PS) II reaction center complexes from plants are presented, and the advantages and disadvantages of each procedure are discussed. One of the simpler 6-Chl procedures and a procedure for isolating 5-Chl complexes are described in detail. Furthermore, a rapid procedure that produces relatively large amounts of less pure 6-Chl material is also described. Criteria to assess the purity of photosystem II (PSII) reaction center preparations are presented, and problems associated with each of the isolation procedures are discussed.
Seibert
Michael
M
Basic Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA.
Yruela
Inmaculada
I
Picorel
Rafael
R
D046911:Macromolecular Substances; D045322:Photosynthetic Reaction Center Complex Proteins / Q000302:isolation & purification*; D045332:Photosystem II Protein Complex / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D010940:Plant Proteins / Q000302:isolation & purification*
D046911:Macromolecular Substances; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins