High-performance liquid chromatographic assay of clonazepam in human plasma using a non-porous silica column.
A new HPLC method has been developed for measuring clonazepam (CZP) in plasma, using a reversed-phase non-porous silica column packed with 2 microm particles. CZP in plasma was first purified with a column extraction technique and injected onto a non-porous silica column. The calibration curve was linear from 5-200 ng/ml. The recoveries of CZP added to plasma were more than 94.0%, with a coefficient of variation in the range of 5.1-13.8%. We developed a rapid routine method using a non-porous silica column that was accurate and improved solvent consumption in the measurement of CZP.
Nakamura
Mitsuhiro
M
Department of Pharmacy, Gifu University Hospital, Yanagido, Japan. mnaka@cc.gifu-u.ac.jp
Fukawa
Kana
K
Sugiyama
Tadashi
T
Katagiri
Yoshihiro
Y
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002998:Clonazepam / Q000097:blood*; D006801:Humans; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000032:analysis*
D002998:Clonazepam; D012822:Silicon Dioxide