Single base oligodeoxyguanosine upregulates Fas ligand release by nonspecific cytotoxic cells.
Nonspecific cytotoxic cells (NCC) are a type of teleost NK-like cell. In the present study a novel stimulus secretion model is described for catfish NCC utilizing single base oligodeoxyguanosine. Binding of guanosine 20-mers (dG20) to NCC up-regulated expression of cytosolic FasL detected by an anti-human FasL monoclonal antibody (mab). In vitro treatment of purified NCC with dG20 produced a 7-fold increase in expression of soluble Fas ligand (sFasL) after 3 h. Antibody binding to NCC was saturable and approximately 30-35% of total NCC were positive for sFasL expression. The teleost FasL equivalent produced programmed cell death of appropriate FasR positive targets. Supernatants from dG20 activated NCC produced hypoploidy and annexin-V binding by FasR bearing HL-60 cells. Treatment of activated supernatants with immobilized anti-FasL mab neutralized these activities. These studies demonstrated that an NK like cell (NCC) produces and secretes sFasL following binding by single base oligodeoxyguanosine.
Kaur
Harjeet
H
Department of Chemical Engineering, North Carolina State University, Raleigh, NC, USA.
Jaso-Friedmann
Liliana
L
Evans
Donald L
DL
D000818:Animals; D017209:Apoptosis / Q000276:immunology; D002397:Catfishes / Q000276:immunology*; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D053938:DNA Fragmentation / Q000276:immunology; D004269:DNA, Bacterial / Q000235:genetics / Q000276:immunology / Q000502:physiology; D003849:Deoxyguanosine / Q000276:immunology*; D053222:Fas Ligand Protein; D005260:Female; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*; D009841:Oligonucleotides / Q000276:immunology; D015854:Up-Regulation; D019014:fas Receptor / Q000276:immunology
D004269:DNA, Bacterial; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D009841:Oligonucleotides; D019014:fas Receptor; D003849:Deoxyguanosine