title: Echinococcus granulosus-specific T-cell lines derived from patients at various clinical stages of cystic echinococcosis.
abstract: To investigate the role of T lymphocytes in the immune response to Echinococcus granulosus, using sheep hydatid fluid (SHF) and antigen B (AgB), we generated T-cell lines from patients with active, transitional and inactive hydatid cysts. We established 16 T-cell lines, eight specific to SHF and eight specific to AgB. At surface phenotyping 88-98% of cells displayed the helper/inducer CD4 antigen. In all patients, at all clinical stages of hydatid cyst disease, T-cell stimulation with SHF and AgB invariably amplified a large number of almost identical Vbeta subfamily fragments. Irrespective of antigen-specificity, the two cell lines from the patient with an inactive cyst had a Th1 profile, because they exclusively expressed and produced IFN-gamma. Conversely, the T-cell lines derived from the seven patients with active and transitional hydatid cysts had mixed Th1/Th2 and Th0 clones. The functional characteristics of the 16 T-cell lines differed markedly in the various clinical stages of cystic echinococcosis, thus providing new in vitro evidence that Th1 lymphocytes contribute decisively to the inactive stage of hydatid disease, Th2 lymphocytes in the active and transitional stages. The parasite-specific T-cell lines, especially the two Th1 lines from the patient with an inactive cyst, may help identify Th1 protective epitopes on SHF and AgB.
authors.affiliation: Department of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Riganò
authors.forename: B
authors.initials: B
authors.lastname: Buttari
authors.forename: E
authors.initials: E
authors.lastname: De Falco
authors.forename: E
authors.initials: E
authors.lastname: Profumo
authors.forename: E
authors.initials: E
authors.lastname: Ortona
authors.forename: P
authors.initials: P
authors.lastname: Margutti
authors.forename: C
authors.initials: C
authors.lastname: Scottà
authors.forename: A
authors.initials: A
authors.lastname: Teggi
authors.forename: A
authors.initials: A
authors.lastname: Siracusano
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D018941:Antigens, CD19 / Q000032:analysis; D017252:CD3 Complex / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D018929:Cell Culture Techniques; D004443:Echinococcosis / Q000276:immunology* / Q000469:parasitology; D004446:Echinococcus / Q000276:immunology*; D005260:Female; D015801:Helminth Proteins / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000032:analysis / Q000096:biosynthesis; D015847:Interleukin-4 / Q000032:analysis / Q000096:biosynthesis; D017493:Leukocyte Common Antigens / Q000032:analysis; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008074:Lipoproteins / Q000276:immunology; D008297:Male; D008875:Middle Aged; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: D018941:Antigens, CD19; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D015801:Helminth Proteins; D008074:Lipoproteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; C089273:antigen B, Echinococcus; D015847:Interleukin-4; D007371:Interferon-gamma; D017493:Leukocyte Common Antigens