Dengue virus serotyping based on envelope and membrane and nonstructural protein NS1 serotype-specific capture immunoglobulin M enzyme-linked immunosorbent assays.
Envelope and membrane (E/M) and nonstructural protein NS1 serotype-specific capture Immunoglobulin M (IgM) enzyme-linked immunosorbent assays (ELISAs) were developed to differentiate four dengue virus serotypes. A total of 93 anti-dengue virus IgM-positive serum samples collected between days 5 and 45 of illness from 59 confirmed dengue patients were analyzed. The results showed that positive serotype specificity could be identified for 86.1 and 47.6% of serum samples tested for E/M-specific IgM antibodies versus 83.3 and 42.9% of serum samples tested for NS1-specific IgM antibodies from patients with primary and secondary dengue virus infections, respectively. Dual analyses with both E/M and NS1 serotype-specific capture IgM ELISAs showed that positive serotype specificity could be correctly identified for 98.6 and 61.9% of all of the primary and secondary serum samples tested, respectively. These findings suggested that E/M and NS1 serotype-specific capture IgM ELISAs have the potential to be of use in dengue virus serotyping.
Shu
Pei-Yun
PY
Division of Laboratory Research and Development, Center for Disease Control, Department of Health, 161, Kun-Yang St., Taipei, Taiwan, Republic of China.
Chen
Li-Kuang
LK
Chang
Shu-Fen
SF
Su
Chien-Ling
CL
Chien
Li-Jung
LJ
Chin
Chuan
C
Lin
Ting-Hsiang
TH
Huang
Jyh-Hsiung
JH
D000914:Antibodies, Viral / Q000097:blood*; D003716:Dengue Virus / Q000145:classification* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood*; D012703:Serotyping; D017361:Viral Nonstructural Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C100315:NS1 protein, Flavivirus; D017361:Viral Nonstructural Proteins