title: The receptor and transporter for internalization of Clostridium botulinum type C progenitor toxin into HT-29 cells.
abstract: Orally ingested botulinum toxin enters the circulatory system and eventually reaches the peripheral nerves, where it elicits a response of neurological dysfunction. In this study, we report the important findings concerning the mechanism of Clostridium botulinum type C progenitor toxin (C16S) endocytic mechanism. C16S toxin bound to high molecular weight proteins on the surface of human colon carcinoma HT-29 cells and was internalized, but not if the cells were pretreated with neuraminidase. Benzyl-GalNAc which inhibited O-glycosylation of glycoproteins also interfered in the toxin's ability to bind the cell surface. On the other hand, the toxin was internalized in spite of pretreatment of the cells with PPMP, an inhibitor of ganglioside synthesis. These results suggest that the glycoproteins, like mucin, fulfill the important roles of receptor and transporter of C16S toxin.
authors.affiliation: Department of Applied Biological Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan. nishikaw@cc.tuat.ac.jp
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nishikawa
authors.forename: Nobuo
authors.initials: N
authors.lastname: Uotsu
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Arimitsu
authors.forename: Jae-Chul
authors.initials: JC
authors.lastname: Lee
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Miura
authors.forename: Yukako
authors.initials: Y
authors.lastname: Fujinaga
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakada
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Watanabe
authors.forename: Tohru
authors.initials: T
authors.lastname: Ohyama
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Sakano
authors.forename: Keiji
authors.initials: K
authors.lastname: Oguma
mesh_terms: D001905:Botulinum Toxins / Q000378:metabolism*; D006031:Glycosylation; D019073:HT29 Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D009439:Neuraminidase / Q000494:pharmacology; D011485:Protein Binding; D021381:Protein Transport
chemical_list: D009439:Neuraminidase; D001905:Botulinum Toxins; C096324:botulinum toxin type C