Proteomic analysis of neuronal hypoxia in vitro.
We used high-throughput Western blotting to identify proteins that are up- or down-regulated by neuronal hypoxia in vitro. Exposure to hypoxia for 24 h produced > or = 1.5-fold increases in the expression of 10/700 proteins (1.4%) and decreases in the expression of 16/700 proteins (2.3%). Up-regulated proteins included Arc, doublecortin/calmodulin kinase-like 1, integrin alpha(v), and fibronectin; down-regulated proteins included nuclear autoantigenic sperm protein, protein kinase C-related kinase 2, and E2F transcription factor 1. The prominence of cytoskeleton-related proteins among those showing altered expression highlights the role of the cytoskeleton in neuronal responses to hypoxia.
Jin
K
K
Buck Institute for Age Research, Novato, California 94945, USA.
Mao
X O
XO
Greenberg
D A
DA
D000818:Animals; D000831:Animals, Newborn; D015687:Cell Hypoxia / Q000502:physiology*; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D020543:Proteome / Q000235:genetics*
D003598:Cytoskeletal Proteins; D009419:Nerve Tissue Proteins; D020543:Proteome