title: Magnetic resonance spectroscopy and chromatographic methods identify altered lipid composition in human renal neoplasms.
abstract: We report on the characterization of the lipid obtained from cortical and medullary normal human kidney tissue, benign renal neoplasms (oncocytoma) and 2 different types of malignant renal neoplasms (chromophobic cell carcinoma and clear cell carcinoma). The total lipid fractions were analyzed by 13C magnetic resonance spectroscopy and thin-layer chromatography, whereas the composition of the total fatty acids and the content of total cholesterol were determined by gas chromatography. alpha-Tocopherol was detected and quantified by high-performance liquid chromatography. The spectroscopic and chromatographic analysis revealed significant differences in the renal tissues examined. It was confirmed that cholesteryl esters (mainly oleate) are typical of clear cell renal carcinomas. Their potential role as prognostic and diagnostic factors is discussed, with particular emphasis on its capability to indicate the tumor diffusion in healthy renal parenchyma. alpha-Tocopherol is prevalent in clear cell carcinoma and it is present in nearly the same low amounts in cortex, medulla and chromophobic cell renal carcinoma. Q10 coenzyme and dolichols were detected by thin-layer chromatography and they are present in significant amounts in the cortex and the benign oncocytoma. Great variations were found in the distribution of saturated and unsaturated fatty acids, especially in the docosapentaenoic, docosahexaenoic and arachidonic acids and the corresponding omega-6/omega-3 fatty acids ratio.
authors.affiliation: Dipartimento di Biochimica, Sezione di Chimica e Propedeutica Biochimica, I-40126 Bologna, Italy. R.Tosi@area.bo.cnr.it
authors.forename: M R
authors.initials: MR
authors.lastname: Tosi
authors.forename: M T
authors.initials: MT
authors.lastname: Rodriguez-Estrada
authors.forename: G
authors.initials: G
authors.lastname: Lercker
authors.forename: A
authors.initials: A
authors.lastname: Poerio
authors.forename: A
authors.initials: A
authors.lastname: Trinchero
authors.forename: A
authors.initials: A
authors.lastname: Reggiani
authors.forename: V
authors.initials: V
authors.lastname: Tugnoli
mesh_terms: D002845:Chromatography; D006801:Humans; D007680:Kidney Neoplasms / Q000737:chemistry*; D008055:Lipids / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D024502:alpha-Tocopherol / Q000032:analysis
chemical_list: D008055:Lipids; D024502:alpha-Tocopherol