title: [Trisomy 3 in intestinal B-cell lymphoma of mucosa-associated lymphoid tissue type].
abstract: OBJECTIVE
  To determine the frequency of trisomy 3 in the intestinal B-cell lymphoma of mucosa associated lymphoid tissue (MALT) type and to explore the relationship between these two.
  
  
  METHODS
  Paraffin embedded tissue sections of intestinal B-cell lymphomas of MALT type, according to WHO classification, were successfully studied. Seven cases were classical MALT lymphoma, being clinically low-grade, and one MALT lymphoma with large cell transform, clinically high-grade. By using biotin labelled DNA probes specific for chromosome 3 centromeric, chromosome in situ hybridization (CISH) were performed. Probe for C16 was used as the positive reference of the method, and chronic intestinal inflammation as the experiment control for detection of C3 copies in tumor cells.
  
  
  RESULTS
  Of the 7 low-grade classical MALT lymphoma, 5 showed trisomy 3 and 2 normal C3. The only case of MALT transformed was trisomy 3. The frequency of trisomy 3 in the intestinal low-grade lymphoma was 71.4%.
  
  
  CONCLUSIONS
  The frequency of trisomy 3 in the intestinal low-grade lymphoma is high, indicating that it may be involved in the pathogenesis of this disease and may be of value for the diagnosis.
authors.affiliation: Department of Pathology, Peking University, Beijing 100083, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Zi-fen
authors.initials: ZF
authors.lastname: Gao
mesh_terms: D000328:Adult; D000368:Aged; D002893:Chromosomes, Human, Pair 3; D005260:Female; D006801:Humans; D007414:Intestinal Neoplasms / Q000235:genetics*; D018442:Lymphoma, B-Cell, Marginal Zone / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D014314:Trisomy