title: Upregulation of glutamate receptors in rat cerebral cortex with neuronal migration disorders.
abstract: Neuronal migration disorders (NMDs) constitute the main pathologic substrate of medically intractable epilepsy in human. This study is designed to investigate the changes in expression of glutamate receptor subtypes on radiation-induced NMD in rats. The lesion was produced by intrauterine irradiation (240 cGy) on E17 rats, and then 10 weeks old rats were used for the study. The pathologic and immuno-histochemical findings for glutamate receptor subunit proteins on NMD cortex were correlated with development of behavioral seizures and EEG abnormality. Spontaneous seizures uncommonly occurred in NMD rats (5%); however, clinical stages of seizures were significantly increased in NMD rats by an administration of kainic acid. Brains taken from irradiated rats revealed gross and histopathologic features of NMD. Focal cortical dysplasia was identified by histopathology and immunohistochemistry with neurofilament protein (NF-M/H). Significantly strong NR1 and NR2A/B immunoreactivities were demonstrated in cytomegalic and heterotopic neurons of NMD rats. The results of the present study indicate that epileptogenesis of NMD might be caused by upregulation of glutamate receptor expression in dysplastic neurons of the rat cerebral cortex with NMDs.
authors.affiliation: Department of Pathology, Chonnam National University Medical School, Gwangju, Korea. mclee@jnu.ac.kr
authors.forename: Min-Cheol
authors.initials: MC
authors.lastname: Lee
authors.forename: Jae-Jin
authors.initials: JJ
authors.lastname: Shim
authors.forename: Jae-Hyoo
authors.initials: JH
authors.lastname: Kim
authors.forename: Myeong-Kyu
authors.initials: MK
authors.lastname: Kim
authors.forename: Young-Jong
authors.initials: YJ
authors.lastname: Woo
authors.forename: Woong-Ki
authors.initials: WK
authors.lastname: Chung
authors.forename: Jung-Jin
authors.initials: JJ
authors.lastname: Suh
authors.forename: Sang-Chae
authors.initials: SC
authors.lastname: Nam
authors.forename: Ji-Shin
authors.initials: JS
authors.lastname: Lee
authors.forename: Yeong-Seon
authors.initials: YS
authors.lastname: Kim
authors.forename: Jin-Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Hyoung-Ihl
authors.initials: HI
authors.lastname: Kim
mesh_terms: D000818:Animals; D002465:Cell Movement; D002540:Cerebral Cortex / Q000378:metabolism*; D004569:Electroencephalography; D018698:Glutamic Acid / Q000378:metabolism; D007150:Immunohistochemistry; D007608:Kainic Acid / Q000494:pharmacology; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D017470:Receptors, Glutamate / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000096:biosynthesis*; D013997:Time Factors; D015854:Up-Regulation
chemical_list: C409466:NR1 NMDA receptor; C120997:NR2A NMDA receptor; C121001:NR2B NMDA receptor; D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; D018698:Glutamic Acid; D007608:Kainic Acid