Poliovirus proves IRES-istible in vivo.
The genetic basis for the attenuation of polio vaccines has been known since the 1980s. Changes in the internal ribosome entry site, within the 5' noncoding region of genomic RNAs, were presumed to reduce translation in certain target organs, leading to the conclusion that attenuation is mediated at the level of translation. A report in this issue of the JCI reveals that poliovirus tropism is, in part, determined after internal ribosome entry.
Semler
Bert L
BL
Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine, California 92697, USA. blsemler@uci.edu
D020121:5' Untranslated Regions; D000818:Animals; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D009690:Nucleic Acid Conformation; D017955:Poliovirus / Q000235:genetics* / Q000502:physiology; D023321:Poliovirus Vaccines; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000737:chemistry; D012045:Regulatory Sequences, Nucleic Acid; D012270:Ribosomes / Q000378:metabolism*
D020121:5' Untranslated Regions; D023321:Poliovirus Vaccines; D012367:RNA, Viral