title: Development of Abeta terminal end-specific antibodies and sensitive ELISA for Abeta variant.
abstract: Alzheimer's disease (AD) is a neurodegenerative affliction associated with memory dysfunction. Senile plaques are a pathological hallmark of AD, and amyloid beta (Abeta) peptides are a major component of these plaques. Abeta peptides are derived from proteolytic cleavage of the Abeta protein precursor (APP) by beta- and gamma-secretases to generate two principal species, Abeta1-40 and Abeta1-42. We have developed antibodies against the N- and C-termini of these peptides, and an ELISA for accurate and sensitive quantitative assessment. Sandwich ELISA composed of N-terminus (Abeta1) end-specific antibody, clone 82E1, and C-termini end-specific antibodies, and clones 1A10 and 1C3 for Abeta40 and Abeta42, respectively, detects full-length Abeta1-40 and 1-42 with a sensitivity in the sub single digit fmol/ml (equivalent to single digit pg/ml) range with no cross-reactivity to APP. A combination of C-termini antibodies and an antibody against the middle region of Abeta detects mouse Abeta in non-transgenic mouse brains.
authors.affiliation: Immuno-Biological Laboratories Co., Ltd., Fujioka-shi, Gunma 375-0005, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Horikoshi
authors.forename: Gaku
authors.initials: G
authors.lastname: Sakaguchi
authors.forename: Amanda G
authors.initials: AG
authors.lastname: Becker
authors.forename: Audrey J
authors.initials: AJ
authors.lastname: Gray
authors.forename: Karen
authors.initials: K
authors.lastname: Duff
authors.forename: Paul S
authors.initials: PS
authors.lastname: Aisen
authors.forename: Haruyasu
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Maeda
authors.forename: Noriaki
authors.initials: N
authors.lastname: Kinoshita
authors.forename: Yasuji
authors.initials: Y
authors.lastname: Matsuoka
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000097:blood / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D001921:Brain / Q000166:cytology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; D000906:Antibodies; D010446:Peptide Fragments