Variations in lipid and apolipoprotein concentrations in human leg lymph: effects of posture and physical exercise.
We studied the variations in the concentrations of cholesterol, triglycerides, phospholipids, apolipoproteins (apos) (A-I, A-II, B, C-III, E), free glycerol and albumin in human prenodal leg lymph during the 24 h cycle. Lymph was collected continuously for up to 96 h from nine healthy males on a low-fat isocaloric diet. In three free-living subjects, all lipid and apolipoprotein concentrations underwent synchronous variations, rising during the night and decreasing during the day. In three subjects who remained in supine rest for 48 h, the amplitude of circadian variation was much smaller. In three who alternated periods of supine rest with upright exercise, the highest concentrations occurred during rest. Lipid, apolipoprotein and albumin concentrations were inversely related to lymph flow rate. Free glycerol, much of which in tissue fluid is derived from local adipocytes, did not follow this pattern. On multiple regression, concentrations in lymph were related independently to the corresponding concentration in plasma (positive) and to lymph flow rate (negative) or lymph albumin concentration (positive). These results show that lipoprotein concentrations in human tissue fluid are determined only partly by their concentrations in plasma. They are also strongly affected by hemodynamic factors via their effects on fluid transport.
Cooke
C Justin
CJ
Department of Cardiovascular Biochemistry, St. Bartholomew's and the Royal London School of Medicine and Dentistry, London, UK.
Nanjee
M Nazeem
MN
Stepanova
Irina P
IP
Olszewski
Waldemar L
WL
Miller
Norman E
NE
D000328:Adult; D000886:Anthropometry; D001053:Apolipoproteins / Q000032:analysis / Q000378:metabolism*; D002784:Cholesterol / Q000032:analysis / Q000378:metabolism; D015444:Exercise / Q000502:physiology; D006801:Humans; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis; D035002:Lower Extremity; D008208:Lymphatic System / Q000378:metabolism*; D008297:Male; D010743:Phospholipids / Q000032:analysis / Q000378:metabolism; D011187:Posture; D012016:Reference Values; D014280:Triglycerides / Q000032:analysis / Q000378:metabolism
D001053:Apolipoproteins; D008055:Lipids; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol