title: Increased levels of gangliosides in the plasma and ascitic fluid of patients with advanced ovarian cancer.
abstract: OBJECTIVE
  To assess the expression of total gangliosides in primary ovarian cancer cell lines, ascitic fluid and plasma of advanced ovarian cancer patients.
  
  
  METHODS
  A prospective study.
  
  
  METHODS
  The Department of Obstetrics and Gynecology at the University of Arkansas for Medical Sciences and the Laboratory of Glycolipid Immunotherapy, John Wayne Cancer Institute.
  
  
  METHODS
  Twenty-two women diagnosed with advanced ovarian cancer and seven normal female controls.
  
  
  METHODS
  Total gangliosides shedding from primary ovarian cancer cell lines were measured by estimating lipid-associated sialic acids (LASAs test) and compared with the ganglioside levels shed by primary cervical and uterine cancer cell lines. In addition, plasma and ascitic samples from advanced ovarian cancer patients were collected at the time of surgery and analysed for the presence of total gangliosides.
  
  
  METHODS
  Levels of total ganglioside in plasma and ascites fluid samples drawn from ovarian cancer patients, relative to total gangliosides levels in plasma from normal female controls.
  
  
  RESULTS
  All primary ovarian tumours secreted high levels of total gangliosides (mean 4 mg/mL, range between 2.7 and 4.8 mg/mL/10(5) cells/24 h) when compared with primary cervical cancers (mean 1.4 mg/mL, range between 0.7 and 2.2 mg/mL/10(5) cells/24 h) (P < 0.008) and uterine carcinoma cell lines (mean 1.4 mg/mL, range between 1.3 and 1.6 mg/mL/10(5) cells/24 h) (P <.004). Elevated levels of total gangliosides were detected in the plasma [mean (SD) 31 (12) mg/mL, range between 18 and 57 mg/mL] (P <.001), and in the peritoneal fluid [mean (SD) 27 (9) mg/mL, range between 14 and 40 mg/mL] (P <.003) of ovarian cancer patients when compared with the levels detectable in the plasma samples of normal female controls tested [mean (SD) 15 (2) mg/mL, range between 12 and 18 mg/mL].
  
  
  CONCLUSIONS
  Increased serum levels of total gangliosides may reflect shedding or release of gangliosides from the surface of ovarian tumour cells. Secretion of gangliosides may play an important role in the inhibition of anti-tumour immune function commonly observed in advanced ovarian cancer.
authors.affiliation: Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA.
authors.forename: Alessandro D
authors.initials: AD
authors.lastname: Santin
authors.forename: Mepur H
authors.initials: MH
authors.lastname: Ravindranath
authors.forename: Stefania
authors.initials: S
authors.lastname: Bellone
authors.forename: Sakunthala
authors.initials: S
authors.lastname: Muthugounder
authors.forename: Michela
authors.initials: M
authors.lastname: Palmieri
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: O'Brien
authors.forename: Juan
authors.initials: J
authors.lastname: Roman
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Cannon
authors.forename: Sergio
authors.initials: S
authors.lastname: Pecorelli
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001202:Ascitic Fluid / Q000737:chemistry*; D045744:Cell Line, Tumor; D005260:Female; D005732:Gangliosides / Q000032:analysis*; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000097:blood / Q000378:metabolism*; D011446:Prospective Studies
chemical_list: D005732:Gangliosides