title: A cDNA microarray technique applied for analysis of global gene expression profiles in tributyltin-exposed ascidians.
abstract: To analyze global gene expressions, we constructed a cDNA microarray from a basal chordate, the ascidian Ciona intestinalis. Ciona is a cosmopolitan species and a genomic analysis of Ciona revealed that ascidians had approximately 15,500 protein-coding genes. Our "Ciona intestinalis cDNA chip version 1 (Ci cDNA chip ver. 1)" has arrayed 13,400 unique Ciona cDNAs. To establish a detection system for gene expression profiles in wild ascidians using a cDNA microarray, we analyzed gene expressions in the whole body of Ciona adults after exposure to 100 nM tributyltin (TBT) for 24 h. In our preliminary array data using Ci cDNA chip ver. 1, we found more than 200 genes that showed strong differential expressions. These genes encoded proteins that were concerned with stress response, detoxification, oxidoreduction reaction, biosynthesis, and catabolism. This, the first large cDNA microarray of this animal, should facilitate analyses of global gene expressions following exposure to TBT.
authors.affiliation: CREST, Japan Science and Technology Corporation, Tokyo, Japan. kaoru@pharm.hokudai.ac.jp
authors.forename: Kaoru
authors.initials: K
authors.lastname: Azumi
authors.forename: Manabu
authors.initials: M
authors.lastname: Fujie
authors.forename: Takeshi
authors.initials: T
authors.lastname: Usami
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Miki
authors.forename: Nori
authors.initials: N
authors.lastname: Satoh
mesh_terms: D000818:Animals; D002938:Ciona intestinalis / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling / Q000379:methods; D007564:Japan; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010140:Pacific Ocean; D014220:Trialkyltin Compounds / Q000633:toxicity*
chemical_list: D014220:Trialkyltin Compounds; C011559:tributyltin