title: [Effects of pentylenetetrazol at subconvulsant and convulsant dose on NMDA receptor subunits in rats].
abstract: To investigate the expression of N-methyl-D-asparate (NMDA) receptor subunit proteins after administration of different doses of pentylenetetrazol (PTZ).
  
  
  
  After ip injection of a subconvulsant (35 mg/kg) and convulsant (50 mg/kg) dose of PTZ, the rats were decapitated at different time points. The levels of cortical NR1  NR2A and NR2B subunit proteins were detected by immunoblotting.
  
  
  
  35 mg/kg PTZ and 50 mg/kg PTZ elicited different behavioral changes (P<0.001). The NR2A subunit in the cortex significantly increased 1 h after PTZ injection (P<0.05). For the 50 mg/kg group, both the NR2A and NR2B subunits proteins increased at 1 h in the cortex and then decreased; the protein levels returned to normal after 48 h. However, NR1 subunit had no changes.
  
  
  
  The NR2 subunit is involved in PTZ-induced seizure.
authors.affiliation: Department of Neurobiology, College of Medicine, Zhejiang University, Hangzhou 310031, China.
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Zhu
authors.forename: Min-zhen
authors.initials: MZ
authors.lastname: Wang
authors.forename: Xiao-hua
authors.initials: XH
authors.lastname: Wu
authors.forename: Shu-jun
authors.initials: SJ
authors.lastname: Xu
authors.forename: Jian-hong
authors.initials: JH
authors.lastname: Luo
authors.forename: Zhong
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D008297:Male; D010433:Pentylenetetrazole / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000032:analysis*; D012640:Seizures / Q000139:chemically induced* / Q000378:metabolism
chemical_list: D010433:Pentylenetetrazole; D016194:Receptors, N-Methyl-D-Aspartate; C409466:NR1 NMDA receptor; C121001:NR2B NMDA receptor