title: Acute and chronic changes of vascular endothelial growth factor (VEGF) in induced sputum of toluene diisocyanate (TDI)-induced asthma patients.
abstract: Vascular endothelial growth factor (VEGF) is a multi-functional cytokine involved in inflammation, repair and angiogenesis in asthmatic airway. This study aimed to evaluate the role of VEGF in immediate bronchoconstriction induced by TDI inhalation, and in chronic TDI-asthma patients. 11 newly diagnosed TDI-asthma patients (group I), 12 chronic TDI-asthma patients with persistent asthma symptoms followed for >4 yr and 15 unexposed healthy controls were enrolled. In group I, induced sputum and serum were collected before and 7 hr after placebo- and TDI-bronchoprovocation test (BPT). In group II, induced sputum and serum were collected every 2 yr. VEGF levels were measured by ELISA. There were no significant differences in sputum and serum VEGF levels between patients and controls. Before and after placebo and TDI-BPT, no significant changes were noted in sputum and serum VEGF levels of group I. In group II patients, sputum VEGF showed variable changes at 1-yr, then decreased significantly at 2-yr (p<0.05), while serum VEGF showed variable changes at 2-yr, which decreased significantly at 4-yr (p<0.05). These results suggest that VEGF may play a minor role in immediate bronchoconstriction after TDI-BPT. In chronic TDI-asthma, VEGF may be involved to 2 yr after the diagnosis and the contribution may decrease after then.
authors.affiliation: Department of Allergy and Rheumatology, Ajou University School of Medicine, Suwon, Korea.
authors.forename: Jeong-Hee
authors.initials: JH
authors.lastname: Choi
authors.forename: Yu-Jin
authors.initials: YJ
authors.lastname: Suh
authors.forename: Soo-Keol
authors.initials: SK
authors.lastname: Lee
authors.forename: Chang-Hee
authors.initials: CH
authors.lastname: Suh
authors.forename: Dong-Ho
authors.initials: DH
authors.lastname: Nahm
authors.forename: Hae-Sim
authors.initials: HS
authors.lastname: Park
mesh_terms: D000328:Adult; D001249:Asthma / Q000139:chemically induced* / Q000378:metabolism*; D001980:Bronchi / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D015444:Exercise; D006801:Humans; D016210:Methacholine Chloride / Q000494:pharmacology; D008875:Middle Aged; D010919:Placebos; D013183:Sputum / Q000378:metabolism*; D013997:Time Factors; D014051:Toluene 2,4-Diisocyanate / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D010919:Placebos; D042461:Vascular Endothelial Growth Factor A; D016210:Methacholine Chloride; D014051:Toluene 2,4-Diisocyanate