4 Hz EMF treated physiological solution depresses Ach-induced neuromembrane current.
The effect of 4 Hz EMF treated physiological solution (PS) on acetylcholine (Ach) sensitivity of the snail neuron was studied. The 4 Hz EMF treated normal PS at room temperature (23 degrees C) has a depressing effect on Ach induced current, while in cold medium (12 degrees C) this effect disappeared. EMF treated, ouabain containing, K-free PS elevates the Ach-induced current at room temperature. It is suggested that the metabotropic effect of EMF treated PS is due to the activation of cGMP-dependent Na:Ca exchange, leading to the decrease of the number of functional active receptors in the membrane, through Na-K pump-induced cell shrinkage, and to increase the receptors affinity to Ach, as the result of decrease of intracellular Ca concentration.
Ayrapetyan
S N
SN
UNESCO Chair-Life Sciences International Educational Center, Yerevan, Armenia. life@arminco.com
Hunanyan
A Sh
ASh
Hakobyan
S N
SN
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects*; D048429:Cell Size / Q000187:drug effects; D006152:Cyclic GMP / Q000378:metabolism; D004553:Electric Conductivity; D004574:Electromagnetic Fields; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006372:Helix, Snails; D007552:Isotonic Solutions / Q000494:pharmacology / Q000528:radiation effects*; D009474:Neurons / Q000187:drug effects*; D010042:Ouabain / Q000494:pharmacology; D011950:Receptors, Cholinergic / Q000187:drug effects*; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013696:Temperature
D004791:Enzyme Inhibitors; D007552:Isotonic Solutions; D011950:Receptors, Cholinergic; D010042:Ouabain; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D006152:Cyclic GMP; D002118:Calcium