Preparation and structure elucidation of alginate oligosaccharides degraded by alginate lyase from Vibro sp. 510.
Alginate that was purified from the fermentation solution of marine bacteria Vibro sp. 510 under specific reaction conditions was hydrolyzed by alginate lyase. Seven oligosaccharides, including di-, tri- and tetrasaccharides, were isolated through low-pressure, gel-permeation chromatography (LP-GPC) and semipreparative strong-anion exchange (SAX) fast-protein liquid chromatography (FPLC). The oligosaccharide structures were elucidated based on ESIMS and 2D NMR spectral analysis. The hydrolytic specificity of this alginate lyase to alginate is discussed.
Zhang
Zhenqing
Z
Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, China.
Yu
Guangli
G
Guan
Huashi
H
Zhao
Xia
X
Du
Yuguo
Y
Jiang
Xiaolu
X
D000464:Alginates / Q000737:chemistry*; D001419:Bacteria / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D020723:Glucuronic Acid / Q000737:chemistry*; D006603:Hexuronic Acids / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D011133:Polysaccharide-Lyases / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
D000464:Alginates; D006603:Hexuronic Acids; D009844:Oligosaccharides; D020723:Glucuronic Acid; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase