[Serological screening of xenogeneic antigens from rat testis].
OBJECTIVE
It is the intent of this study to analyzed the rat testis library by employing a modified SEREX (serological analysis of recombinant cDNA expression library) approach to find the xenogeneic homologous tumor antigens which could be useful in developing cancer vaccines.


METHODS
The screening serum was obtained from the immunized rabbits with human ovarian cancer cells, and 10 positive clones were isolated from the rat testis cDNA library using SEREX technology.


RESULTS
We found these 10 clones encoded seven different proteins by means of bioinformatic analysis. Among them, OV-2 and OV-4 encode proteins related to carcinoma in human, yet OV-6 and OV-7 are novel and their encoded proteins remain unknown.


CONCLUSIONS
This study indicates that the utilization of xenogeneic immunized serum in the serological screening of xenogeneic homologous tumor antigens may expand the application of traditional SEREX technology.
Peng
Feng
F
Key Laboratory of Biotherapy of Human Diseases of Ministry of Education, Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China.
Tian
Ling
L
Deng
Hong-xin
HX
Li
Yu
Y
Yang
Han-shuo
HS
Kan
Bing
B
Zhao
Xia
X
Liao
Jian
J
Wei
Yu-quan
YQ
D000818:Animals; D015478:Antigens, Heterophile / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000276:immunology; D001943:Breast Neoplasms / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics; D051381:Rats; D011994:Recombinant Proteins / Q000276:immunology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012698:Serologic Tests / Q000379:methods; D013737:Testis / Q000276:immunology*
D015478:Antigens, Heterophile; D000951:Antigens, Neoplasm; D018076:DNA, Complementary; D009363:Neoplasm Proteins; D011994:Recombinant Proteins