title: Analysis of chromosomes 8 and 17 aneuploidies in laryngeal squamous cell carcinoma by fluorescence in situ hybridization.
abstract: OBJECTIVE
  The objectives were to investigate chromosomes 8 and 17 numerical aberrations by using fluorescence in situ hybridization in laryngeal squamous cell carcinoma and also to determine whether there is any association between chromosomes 8 and 17 aneuploidies and TNM classification and subgroups of laryngeal squamous cell carcinomas.
  
  
  METHODS
  Descriptive study.
  
  
  METHODS
  Tumor and control samples were taken from 23 patients with LSCC by surgical operation. Fluorescence in situ hybridization analysis with chromosomes 8- and 17-specific alpha-satellite DNA probes was performed on the interphase nuclei.
  
  
  RESULTS
  The percentages for chromosomes 8 and 17 aneuploidies were 33% (SD = 25.7%) (median value, 26.2%; range, 3.5%-81.8%) and 19.2% (SD = 15.8%) (median value, 9.8%; range, 3.6%-63.7%), respectively. There was a significant difference between stage 2 and stage 3 (P <.05) and between stage 2 and stage 4 for chromosome 8 aneuploidy (P <.05) but not for chromosome 17 aneuploidy (P >.05). There was also a significant difference for the T classification for chromosome 8 aneuploidy (P <.05) but not for chromosome 17 (P >.05).
  
  
  CONCLUSIONS
  Chromosome 8 aneuploidy may be related to stage and T classification of laryngeal squamous cell carcinoma and its progression.
authors.affiliation: Departments of Otolaryngology, Meram Medical Faculty, Selçuk University, Konya, Turkey. kayhan@medscape.com
authors.forename: Kayhan
authors.initials: K
authors.lastname: Oztürk
authors.forename: Hasan
authors.initials: H
authors.lastname: Acar
authors.forename: Ercan
authors.initials: E
authors.lastname: Durmuş
authors.forename: Adnan
authors.initials: A
authors.lastname: Oztürk
authors.forename: Necip
authors.initials: N
authors.lastname: Mutlu
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D000782:Aneuploidy; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002886:Chromosomes, Human, Pair 17; D002898:Chromosomes, Human, Pair 8; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D018709:Statistics, Nonparametric