title: fgf17b, a novel member of Fgf family, helps patterning zebrafish embryos.
abstract: Fibroblast growth factors (Fgfs) play important roles in the pattern formation of early vertebrate embryos. We have identified a zebrafish ortholog of human FGF17, named fgf17b. The first phase of fgf17b expression occurs in the blastodermal margin of late blastulae and in the embryonic shield of early gastrulae. The second phase starts after the onset of segmentation, mainly in the presomitic mesoderm and newly formed somites. Injection of fgf17b mRNA into one-cell embryos induces expression of the mesodermal marker no tail (ntl) and rescues ntl expression suppressed by overexpression of lefty1 (lft1). Overexpression of fgf17b dorsalizes zebrafish gastrulae by enhancing expression of chordin (chd), which is an antagonist of the ventralizing signals BMPs. In addition, overexpression of fgf17b posteriorizes the neuroectoderm. Simultaneous knockdown of fgf17b and fgf8 with antisense morpholinos results in reduction of chd and ntl. Knockdown of fgf17b can alleviate inhibitory effect of ectopic expression of fgf3 on otx1. These data together suggest that Fgf17b plays a role in early embryonic patterning. We also demonstrate that fgf17b and fgf8 have stronger mesoderm inducting activity than fgf3, whereas fgf17b and fgf3 have stronger activity in posteriorizing the neuroectoderm than fgf8. Like fgf8, activation of fgf17b expression depends on Nodal signaling.
authors.affiliation: Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane & Membrane Biotechnology, Tsinghua University, Beijing 100084, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Cao
authors.forename: Jue
authors.initials: J
authors.lastname: Zhao
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Sun
authors.forename: Zhixing
authors.initials: Z
authors.lastname: Zhao
authors.forename: John
authors.initials: J
authors.lastname: Postlethwait
authors.forename: Anming
authors.initials: A
authors.lastname: Meng
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D019521:Body Patterning / Q000235:genetics / Q000502:physiology*; D002874:Chromosome Mapping; D017931:DNA Primers; D005326:Fetal Proteins; D051518:Fibroblast Growth Factor 3; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors / Q000235:genetics* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D017403:In Situ Hybridization; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D055458:Left-Right Determination Factors; D008845:Microinjections; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020825:T-Box Domain Proteins / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology*; D029961:Zebrafish Proteins / Q000378:metabolism; D000098908:Brachyury Protein
chemical_list: D017931:DNA Primers; D005326:Fetal Proteins; D051518:Fibroblast Growth Factor 3; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D055458:Left-Right Determination Factors; D020825:T-Box Domain Proteins; D016212:Transforming Growth Factor beta; D029961:Zebrafish Proteins; D000098908:Brachyury Protein; C484724:FGF17 protein, human; C526161:LEFTY1 protein, human; C489200:fgf3 protein, zebrafish; C484725:fibroblast growth factor 17, zebrafish; C054234:chordin