title: High-performance liquid chromatographic assay of clonazepam in human plasma using a non-porous silica column.
abstract: A new HPLC method has been developed for measuring clonazepam (CZP) in plasma, using a reversed-phase non-porous silica column packed with 2 microm particles. CZP in plasma was first purified with a column extraction technique and injected onto a non-porous silica column. The calibration curve was linear from 5-200 ng/ml. The recoveries of CZP added to plasma were more than 94.0%, with a coefficient of variation in the range of 5.1-13.8%. We developed a rapid routine method using a non-porous silica column that was accurate and improved solvent consumption in the measurement of CZP.
authors.affiliation: Department of Pharmacy, Gifu University Hospital, Yanagido, Japan. mnaka@cc.gifu-u.ac.jp
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Nakamura
authors.forename: Kana
authors.initials: K
authors.lastname: Fukawa
authors.forename: Tadashi
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Katagiri
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002998:Clonazepam / Q000097:blood*; D006801:Humans; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000032:analysis*
chemical_list: D002998:Clonazepam; D012822:Silicon Dioxide