title: DNA strand breakage in mussels (Mytilus edulis L.) deployed in intertidal and subtidal zone in Reykjavík harbour.
abstract: DNA single-strand breaks were analysed in the blue mussel (Mytilus edulis L.) deployed in intertidal and subtidal zones in the PAH contaminated Reykjavík harbour and at a reference site, Hvalfjörethur, Iceland. DNA strand breaks were analysed by Comet assay in isolated gill and haemocyte cells from six mussels from each site and depth. Increased DNA damage in both gill cells and haemocytes were observed in mussels deployed in Reykjavík harbour compared to the reference site. Intertidal mussels from Reykjavík harbour had higher DNA damage in haemocytes compared to subtidal mussels. The Comet assay seems to be useful for measuring genotoxic exposure in mussels from the field, and that DNA damage might be higher in the intertidal zone either due to higher exposure to contaminants or because of physiological and biochemical responses to variations in oxygen availability.
authors.affiliation: Institute of Applied Environmental Research (ITMx), Stockholm University, S-106 91 Stockholm, Sweden. halldor@hi.is
authors.forename: Halldór P
authors.initials: HP
authors.lastname: Halldórsson
authors.forename: Gunilla
authors.initials: G
authors.lastname: Ericson
authors.forename: Jörundur
authors.initials: J
authors.lastname: Svavarsson
mesh_terms: D000818:Animals; D001267:Atlantic Ocean; D049872:Bivalvia / Q000235:genetics / Q000378:metabolism*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects* / Q000235:genetics; D004781:Environmental Exposure; D004784:Environmental Monitoring / Q000379:methods*; D005880:Gills / Q000378:metabolism; D006434:Hemocytes / Q000378:metabolism; D007055:Iceland; D010100:Oxygen / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D010100:Oxygen