Preparative scale purification of shidasterone, 2-deoxy-polypodine b and 9a,20-dihydroxyecdysone from Silene italica ssp. nemoralis.
A suitable combination of preparative scale separation methods results in effective clean-up of the ecdysteroids of Silene italica ssp. nemoralis (Waldst. and Kit.) Nyman. The isolation of minor ecdysteroids from the partially purified extract is based on the use of both droplet counter-current chromatography and low-pressure reversed-phase liquid chromatography. The purification is completed by preparative thin-layer chromatography and preparative high-performance liquid chromatography to obtain the minor ecdysteroids, such as 2-deoxy-20-hydroxyecdysone, shidasterone, 2-deoxy-polypodine B, makisterone C, and 9alpha,20-dihydroxyecdysone.
Báthori
Mária
M
Department of Pharmacognosy, University of Szeged, Eötvös utca 6, H-6720 Szeged, Hungary. bathori@pharma.szote.u-szeged.hu
Pongrácz
Zita
Z
Omacht
Róbert
R
Máthé
Imre
I
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003377:Countercurrent Distribution; D004440:Ecdysone / Q000031:analogs & derivatives* / Q000302:isolation & purification*; D026461:Ecdysteroids / Q000302:isolation & purification*; D029751:Silene / Q000737:chemistry*; D013329:Structure-Activity Relationship
C481865:2-deoxy-5beta,20-dihydroxyecdysone; C476839:9alpha,20-dihydroxyecdysone; D026461:Ecdysteroids; C011352:shidasterone; D004440:Ecdysone