title: Oxidative stress in neonates: evaluation using specific biomarkers.
abstract: Increased oxidative stress has been implicated in pathogenesis of serious diseases in neonates. We measured urinary levels of 8-hydroxy-2'-deoxyguanosine (a marker of oxidative DNA damage), acrolein-lysine adduct (a marker of lipid peroxidation and oxidative protein damage), and nitrite/nitrate (a marker of endogenous nitric oxide formation) in one-month-old neonates to examine the status of oxidative stress and its relationship to the degree of prematurity and clinical condition in neonates. Study subjects comprised three groups: healthy term neonates, clinically stable preterm neonates requiring no supplemental oxygen, and clinically sick preterm neonates requiring supplemental oxygen and ventilator support. Urinary levels of 8-hydroxy-2'-deoxyguanosine and acrolein-lysine adduct were significantly higher in sick preterm neonates than those of stable preterm and healthy term neonates. In the sick preterm group, neonates developing active retinopathy showed significantly higher levels of acrolein-lysine adduct than the other neonates without retinopathy. There were no significant differences in both urinary markers of oxidative stress between stable preterm and healthy term neonates. The urinary nitrite/nitrate levels were not significantly different among the three groups, suggesting no difference in endogenous nitric oxide formation. Collectively, these results provide evidence of augmentation of oxidative damage to DNA, lipids and proteins, especially in clinically sick preterm neonates.
authors.affiliation: Department of Pediatrics, Fukui Medical University, Fukui 910-1193, Japan. htsuka@fmsrsa.fukui-med.ac.jp
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Tsukahara
authors.forename: Mi-Zu
authors.initials: MZ
authors.lastname: Jiang
authors.forename: Naoko
authors.initials: N
authors.lastname: Ohta
authors.forename: Shuko
authors.initials: S
authors.lastname: Sato
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tamura
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hiraoka
authors.forename: Masayuki
authors.initials: M
authors.lastname: Maeda
authors.forename: Mitsufumi
authors.initials: M
authors.lastname: Mayumi
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000171:Acrolein / Q000378:metabolism; D015415:Biomarkers; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D015227:Lipid Peroxidation; D008239:Lysine / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000502:physiology; D018384:Oxidative Stress
chemical_list: D015415:Biomarkers; D009569:Nitric Oxide; D000171:Acrolein; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D008239:Lysine