[Construction of eukaryotic expression vector of murine SLC gene and characterization of its chemotactic function].
OBJECTIVE
To construct the eukaryotic expression vector of murine SLC gene and study the chemotactic function of murine SLC in-vitro and in-vivo.


METHODS
Murine SLC gene was cloned by RT-PCR from the thymus tissue of a C57BL/6 mouse. Eukaryotic expression vector of SLC gene-pcDNA3.1 mSLC was constructed and transfected into B16F10 cells by gene gun. Culture supernatant was collected 48 hours after the transfection and chemotactic function of expression product to lymphocytes was detected by a chemotaxis chamber. SLC expression was detected by RT-PCR. Lymphocytic infiltration was observed in SLC gene transfected murine abdominal skin.


RESULTS
The gene cloned from the C57BL/6 mouse thymus tissue was SLC gene Scya21b. Transfected cells expressed SLC mRNA, and culture supernatant of those cells had a potent chemotactic function to lymphocytes. Histological examination of transfected skin showed obvious lymphocytic infiltration.


CONCLUSIONS
The SLC gene was cloned from the mouse thymus tissue and could be expressed in B16F10 cells. The expression product in-vitro and in-vivo had a remarkable chemotactic function to lymphocytes.
Ma
Fei
F
Cancer Hospital &, Cancer Institute, Chinese Academy of Medical Sciences, Beijing 100021,China. mcmf@sina.com
Zhang
Shu-ren
SR
Ning
Li
L
Sun
Wen-xin
WX
Liang
Xiao
X
Zhang
Xue-yan
XY
Fu
Ming
M
Lin
Chen
C
D000818:Animals; D054421:Chemokine CCL21; D019742:Chemokines, CC / Q000235:genetics* / Q000502:physiology; D002634:Chemotaxis, Leukocyte; D003001:Cloning, Molecular; D005822:Genetic Vectors; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000473:pathology; D014162:Transfection
C515479:Ccl21c protein, mouse; D054421:Chemokine CCL21; D019742:Chemokines, CC