title: Beta-catenin associates with human parainfluenza virus type 3 ribonucleoprotein complex and activates transcription of viral genome RNA in vitro.
abstract: Several studies have indicated that human parainfluenza virus type 3 (HPIV-3) requires polymeric actin for transcription of its genome RNA in vitro and in vivo. In the current study, we have identified beta-catenin, an actin-bound protein, as one of the transcriptional activators for HPIV-3 genome RNA. Beta-catenin was packaged within the purified HPIV-3 virions and was associated with the HPIV-3 ribonucleoproteins (RNP) from infected cells. Moreover, purified beta-catenin interacted with bacterially expressed HPIV-3 nucleocapsid protein (N) and phosphoprotein (P) fused to glutathione S-transferase (GST). Double-labeled immunofluorescent confocal microscopic analysis revealed colocalization of beta-catenin with HPIV-3 RNP at cell periphery in infected cells. The HPIV-3 RNP-associated beta-catenin functioned as a transactivator of HPIV-3 genome, because purified beta-catenin stimulated transcription of viral RNP in an in vitro transcription assay. These results demonstrate that beta-catenin, a multifunctional protein that is involved in cell-cell adhesion and embryogenesis, acts as one of the transcriptional activators of HPIV-3 genome RNA.
authors.affiliation: Department of Virology, The Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA. boses@ccf.org
authors.forename: Santanu
authors.initials: S
authors.lastname: Bose
authors.forename: Amiya K
authors.initials: AK
authors.lastname: Banerjee
mesh_terms: D003598:Cytoskeletal Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D006801:Humans; D010224:Parainfluenza Virus 3, Human / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D012367:RNA, Viral / Q000096:biosynthesis*; D012261:Ribonucleoproteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation / Q000235:genetics*; D051176:beta Catenin
chemical_list: C495270:CTNNB1 protein, human; D003598:Cytoskeletal Proteins; D012367:RNA, Viral; D012261:Ribonucleoproteins; D015534:Trans-Activators; D051176:beta Catenin