[The cloning of human augmentor of liver regeneration-interacting proteins].
OBJECTIVE
To screen human augmentor of liver regeneration(hALR)-interacting proteins and to explore the mechanisms of hALR in liver regeneration.


METHODS
The open reading frame of hALR was used to construct the "bait" plasmid and the genes encoding hALR-interacting proteins were screened from the human liver cDNA library that was pretransformed into yeast Y187 by yeast two-hybrid system. Bioinformatic analysis of the sequences of the positive clones were performed.


RESULTS
The positive clones encoding metallothionein, albumin, selenoprotein P,Na/K-ATPase, and 2 unknown proteins were screened out.


CONCLUSIONS
The successful cloning of the genes encoding proteins interacting with hALR may pave the way for studying the interaction between the above proteins and hALR, and the molecular mechanisms of biological effect of hALR.
Tong
Ming-hua
MH
Center of Infectious Diseases, The 458th Hospital of PLA, Guangzhou 510602, China. minghuat@163.net
Chen
Si-qiang
SQ
Yao
Ru-hua
RH
Kong
Xiang-ping
XP
D000418:Albumins / Q000235:genetics; D003001:Cloning, Molecular; D015723:Gene Library; D006801:Humans; D008668:Metallothionein / Q000235:genetics; D011506:Proteins / Q000235:genetics / Q000502:physiology*; D051149:Selenoprotein P; D051140:Selenoproteins; D017422:Sequence Analysis, DNA; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics; D020798:Two-Hybrid System Techniques; D050862:Oxidoreductases Acting on Sulfur Group Donors
D000418:Albumins; D008668:Metallothionein; D011506:Proteins; D051149:Selenoprotein P; D051140:Selenoproteins; D000254:Sodium-Potassium-Exchanging ATPase; C088825:GFER protein, rat; D050862:Oxidoreductases Acting on Sulfur Group Donors