title: Use of a recombinant envelope protein subunit antigen for specific serological diagnosis of West Nile virus infection.
abstract: Serological diagnosis of West Nile virus (WNV) infection is complicated by extensive antigenic cross-reactivity with other closely related flaviviruses, such as St. Louis encephalitis virus. Here we describe a recombinant, bacterially expressed antigen equivalent to structural domain III of the WNV envelope protein that has allowed clear discrimination of antibody responses to WNV from those against other related flaviviruses in indirect enzyme-linked immunosorbent assays using standardized control antisera and field-collected samples.
authors.affiliation: Department of Pathology, UTMB, 301 University Blvd., Galveston, TX 77555-0609, USA.
authors.forename: David W C
authors.initials: DW
authors.lastname: Beasley
authors.forename: Michael R
authors.initials: MR
authors.lastname: Holbrook
authors.forename: Amelia P A
authors.initials: AP
authors.lastname: Travassos Da Rosa
authors.forename: Lark
authors.initials: L
authors.lastname: Coffey
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Carrara
authors.forename: Kathrine
authors.initials: K
authors.lastname: Phillippi-Falkenstein
authors.forename: Rudolf P
authors.initials: RP
authors.lastname: Bohm
authors.forename: Marion S
authors.initials: MS
authors.lastname: Ratterree
authors.forename: Kristy M
authors.initials: KM
authors.lastname: Lillibridge
authors.forename: George V
authors.initials: GV
authors.lastname: Ludwig
authors.forename: Jose
authors.initials: J
authors.lastname: Estrada-Franco
authors.forename: Scott C
authors.initials: SC
authors.lastname: Weaver
authors.forename: Robert B
authors.initials: RB
authors.lastname: Tesh
authors.forename: Robert E
authors.initials: RE
authors.lastname: Shope
authors.forename: Alan D T
authors.initials: AD
authors.lastname: Barrett
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D021122:Protein Subunits; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D012698:Serologic Tests; D014901:West Nile Fever / Q000175:diagnosis*; D014902:West Nile virus / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D021122:Protein Subunits; D011994:Recombinant Proteins