Molecular cloning of hepatic glucose-6-phosphatase catalytic subunit from gilthead sea bream (Sparus aurata): response of its mRNA levels and glucokinase expression to refeeding and diet composition.
To examine the relationship between structure and function of glucose-6-phosphatase (G6Pase) in fish, we undertook molecular cloning and modulation of G6Pase expression by starvation and refeeding on diets with different nutrient composition in the liver of the carnivorous fish, Sparus aurata. A cDNA encoding the full-length G6Pase catalytic subunit from the liver of S. aurata was isolated. This cDNA encodes a 350-amino acid protein, with low homology to the mammalian G6Pase, although it contains most of the key residues required for catalysis. Based on hydrophobicity and membrane structure prediction, we propose a model containing nine-transmembrane regions for S. aurata G6Pase. Northern blots showed that refeeding after a prolonged starvation rapidly reverses the glucose/glucose-6-phosphate substrate cycle flux in the fish liver through decreased G6Pase expression and strong glucokinase (GK) induction. The effect of refeeding different diets on G6Pase and GK expression, indicated that hepatic intermediary metabolism of fish fed diets with low protein/high carbohydrate diets is impelled towards utilization of dietary carbohydrates, by means of modulation of GK mRNA levels rather than G6Pase expression. These findings challenge the role attributed to dysregulation of G6Pase or GK expression in the low ability of carnivorous fish to metabolise glucose.
Metón
Isidoro
I
Departament de Bioquímica i Biologia Molecular, Facultat de Farmàcia, Universitat de Barcelona, Avgda. Diagonal 643, 08028 Barcelona, Spain.
Caseras
Anna
A
Fernández
Felipe
F
Baanante
Isabel V
IV
D000595:Amino Acid Sequence; D000821:Animal Feed; D001483:Base Sequence; D020134:Catalytic Domain; D003001:Cloning, Molecular; D004032:Diet; D005941:Glucokinase / Q000235:genetics* / Q000378:metabolism; D005952:Glucose-6-Phosphatase / Q000235:genetics* / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D021541:Sea Bream / Q000502:physiology*; D017386:Sequence Homology, Amino Acid
D012333:RNA, Messenger; D005941:Glucokinase; D005952:Glucose-6-Phosphatase