Normal differentiation and functions of mouse dendritic cells derived from RAG-deficient bone marrow progenitors.
Dendritic cells (DC) mature upon infectious agent detection to elicit immune responses. It has been suggested that T cells influence peripheral DC function. However, it is not known if lymphocytes influence DC progenitors. Therefore, we determined the ability of bone marrow progenitors from T and B cell-deficient mice to generate functional DC. We report that bone marrow-derived DC from RAG-2(-/-) mice differentiate and proliferate normally. Moreover, such generated DC efficiently internalize particles, mature in response to various Toll-like receptor engagement, and activate allogenic T cells. This work strongly supports that early signals delivered during DC ontogeny by mature lymphocytes do not influence the functional differentiation of DC progenitors.
Faure
Mathias
M
Laboratoire d'Immunochimie, Institut National de la Santé et de la Recherche Médicale, Unité 548, Commissariat à l'Energie Atomique de Grenoble, Université Joseph Fourier, 38000 Grenoble, France. mfaure@cea.fr
Villiers
Christian L
CL
Marche
Patrice N
PN
D000818:Animals; D002454:Cell Differentiation; D004268:DNA-Binding Proteins / Q000235:genetics; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D004705:Endocytosis; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D013601:T-Lymphocytes / Q000276:immunology
D004268:DNA-Binding Proteins; C499308:Rag2 protein, mouse; C064655:V(D)J recombination activating protein 2