title: Isolation of photosystem I reaction center subunit polypeptides from spinach.
abstract: Methods to prepare photosystem (PS)I reaction center (RC) subunit polypeptides are described. For PsaC, PsaD, PsaE, and PsaY, the filtrate resulting from the ultrafiltration in Chapter 6 is used for preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). PsaA and PsaB, Type II PSI particles, prepared as described in the preceding chapter, are treated with sodium dodecyl sulfate, then applied on preparative SDS-PAGE. After electrophoresis, each band is cut out and electroeluted. A simple method to prepare PsaC is also presented.
authors.affiliation: Department of Biochemistry and Molecular Biology, Saitama University, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Hiyama
mesh_terms: D010455:Peptides / Q000302:isolation & purification; D045322:Photosynthetic Reaction Center Complex Proteins / Q000302:isolation & purification*; D045331:Photosystem I Protein Complex / Q000737:chemistry*; D010940:Plant Proteins / Q000302:isolation & purification; D021122:Protein Subunits / Q000302:isolation & purification*; D018724:Spinacia oleracea / Q000737:chemistry*
chemical_list: D010455:Peptides; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins; D021122:Protein Subunits