Phospholipase C-dependent phosphoinositide breakdown induced by ELF-EMF in Peganum harmala calli.
With the aim of examining the response of plant cells to extremely low frequency (ELF) electromagnetic fields (EMF), we investigated the behaviour of the phosphatidylinositol 4,5 bisphosphate (PtdIns 4,5-P(2)) molecule (the precursor of the phosphoinositide signal transduction cascade) by exposing callus cells from Peganum harmala to 50 Hz, 1 gauss EMF for 10 min and by examining the level and the fatty acid composition of PtdIns 4,5-P(2) after the exposure. Our results evidenced a statistically significant decrease in PtdIns 4,5-P(2) concentrations and a different involvement of the constituting fatty acids in the induced breakdown. The manipulation of the lipid-based signalling pathway by phosphoinositide-phospholipase C (PI-PLC) inhibitors (i.e., neomycin, U-73122 and ET-18-OCH(3)) seems to support the hypothesis that, as in animals, also in plants, the cell membrane is the primary impact site of ELF electromagnetic stimulus and that this interaction could probably involve the activation of PI signal transduction pathway including a heterotrimeric G protein.
Piacentini
Maria Piera
MP
Istituto di Chimica Biologica Giorgio Fornaini, Università degli Studi di Urbino, Via Saffi 2, 61029 Urbino, Italy.
Piatti
Elena
E
Fraternale
Daniele
D
Ricci
Donata
D
Albertini
Maria Cristina
MC
Accorsi
Augusto
A
D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004574:Electromagnetic Fields; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism; D032443:Peganum / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism / Q000528:radiation effects*; D018514:Plant Structures / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D012639:Seeds / Q000737:chemistry; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors / Q000378:metabolism*
D000975:Antioxidants; D004791:Enzyme Inhibitors; D005227:Fatty Acids; D019269:Phosphatidylinositol 4,5-Diphosphate; D010738:Type C Phospholipases; D001205:Ascorbic Acid