Evaluation of the antioxidant effects of some flavonylthiazolidinediones by determining their effects on lipid peroxidation, superoxide anion formation, and 2,2-diphenyl-1-picrylhydrazyl stable free radical.
The in vitro antioxidant effects of some flavonylthiazolidinediones (Ia-d, IIa-d) on rat liver microsomal NADPH-dependent lipid peroxidation (LP) levels were determined by measuring the formation of 2-thiobarbituric acid reactive substance. The free radical-scavenging properties of the compounds were examined in vitro by determining the capacity to scavenge superoxide anion formation and of the interaction with the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). The compounds had no inhibitory effects on LP. However, they had variable inhibitory influence on superoxide anion production and DPPH radical in a concentration-dependent manner. The most active compound, 3-(2,5-dimethoxyphenacyl)-5-[2-phenyl-4H-4-oxo-1-benzopyran-6-yl)methylenyl]-thiazolidine-2,4-dione, Id showed 98% inhibition of superoxide anion production and 95% inhibition of DPPH stable free radical at 10(-3) M.
Tuncbilek
Meral
M
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Ankara University, Turkey. tuncbile@pharmacy.ankara.edu.tr
Can Eke
Benay
B
Ayhan-Kilcigil
Gülgün
G
Coban
Tülay
T
Iscan
Mümtaz
M
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001713:Biphenyl Compounds; D004353:Drug Evaluation, Preclinical / Q000379:methods; D047309:Flavones / Q000737:chemistry / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D010851:Picrates / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism*; D045162:Thiazolidinediones / Q000494:pharmacology*
D000975:Antioxidants; D001713:Biphenyl Compounds; D047309:Flavones; D005609:Free Radicals; D010851:Picrates; D045162:Thiazolidinediones; D013481:Superoxides; C004931:1,1-diphenyl-2-picrylhydrazyl