Human follitropin receptor (FSHR) interacts with the adapter protein 14-3-3tau.
The human follitropin (follicle stimulating hormone, FSH) receptor (FSHR) is a G protein-coupled receptor (GPCR). To identify cytoplasmic proteins that may regulate FSHR function, a yeast-based interaction trap was performed. A linked construct of the first and second intracellular loops (iL1-iL2 bait) of FSHR was used as bait and a human ovarian cDNA library was used as prey. Among the proteins identified that interacted with the bait was 14-3-3tau, a member of a family of homodimeric cytoplasmic adapter proteins. Human granulosa cells, the site of FSHR expression in the ovary, were found to contain 14-3-3tau. Importantly, 14-3-3tau co-immunoprecipitated with FSHR stably expressed in HEK 293 cells. Its association with FSHR was follitropin-dependent. Over-expression of 14-3-3tau resulted in a modest decrease of follitropin-induced cAMP accumulation. Collectively, these data support a role for 14-3-3tau in follitropin action. The finding that 14-3-3tau interacts with FSHR is novel and should lead to new insights into the regulation of GPCR in general and FSHR specifically.
Cohen
Brian D
BD
Wadsworth Center, David Axelrod Institute for Public Health, New York State Department of Health, 120 New Scotland Ave., Albany, NY 12208, USA.
Nechamen
Cheryl A
CA
Dias
James A
JA
D048948:14-3-3 Proteins / Q000235:genetics / Q000378:metabolism*; D020816:Amino Acid Motifs; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D008184:Luteal Cells / Q000378:metabolism; D011485:Protein Binding; D011962:Receptors, FSH / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D020798:Two-Hybrid System Techniques
D048948:14-3-3 Proteins; D011962:Receptors, FSH; D000242:Cyclic AMP