title: Individual-specific variation of gene expression in peripheral blood leukocytes.
abstract: DNA microarray technology is used to determine gene expression profiles of various cell types, especially abnormal cells, such as cancer. By contrast, relatively little attention has been given to expression profiling of normal tissues. Here we describe studies of gene expression in peripheral blood leukocytes (PBL) from normal individuals sampled multiple times over periods ranging from several weeks up to 6 months. We demonstrate stable patterns of gene expression that differ between individuals. Among the genes whose expression varies by individual is a group of genes responsive to interferon stimulation. Certain individuals ( approximately 10-20% of those tested) showed higher baseline levels and lower inducibility of these genes in response to in vitro interferon stimulation. These studies demonstrate the feasibility of using DNA microarrays to measure the variations in gene expression of PBL from different individuals in response to environmental and genetic factors.
authors.affiliation: Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, D4-100, Seattle, WA 98109, USA. jradich@fhcrc.org
authors.forename: Jerald P
authors.initials: JP
authors.lastname: Radich
authors.forename: Mao
authors.initials: M
authors.lastname: Mao
authors.forename: Sergey
authors.initials: S
authors.lastname: Stepaniants
authors.forename: Matt
authors.initials: M
authors.lastname: Biery
authors.forename: John
authors.initials: J
authors.lastname: Castle
authors.forename: Terry
authors.initials: T
authors.lastname: Ward
authors.forename: Greg
authors.initials: G
authors.lastname: Schimmack
authors.forename: Sumire
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Michael
authors.initials: M
authors.lastname: Carleton
authors.forename: Johanna
authors.initials: J
authors.lastname: Lampe
authors.forename: Peter S
authors.initials: PS
authors.lastname: Linsley
mesh_terms: D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D014644:Genetic Variation; D006801:Humans; D007372:Interferons / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D008070:Lipopolysaccharides; D007372:Interferons