title: [Purification of colorectal cancer-associated antigen by immuno-affinity chromatography].
abstract: OBJECTIVE
  To purify and characterize the colorectal cancer-associated antigen from cultured colorectal cancer cells.
  
  
  METHODS
  The colorectal cancer cell lines that highly expressed the associated antigen were selected by flow cytometry with five specific monoclonal antibodies (mAbs) CYL1-5. Western blot was used to determine the binding ability of five mAbs to the associated antigens released from colorectal cells lysed with single or triplex-detergent, respectively. The mAb with highest binding ability was employed as the ligand for the immuno-affinity chromatography. The antigens purified through immuno-affinity chromatography were identified by Western blot.
  
  
  RESULTS
  The associated antigens were highly expressed on the colorectal cancer cell lines Hce-8693. The binding ability of mAb CYL-2 to the antigen was higher than that of CYL-1, CYL-3-5. The purified associated antigen binding to mAb CYL-2 was a heterodimer composed of two subunits with relative molecular mass (M(r)) of 60 x 10(3) and 70 x 10(3), respectively.
  
  
  CONCLUSIONS
  The purified associated-antigen binding to mAb CYL-2 was obtained from the colorectal cancer cell line Hce-8693 through immuno-affinity chromatography with mAb CYL-2.
authors.affiliation: Department of Cell Biology, Fourth Military Medical University, Xi'an 710032, China. fmmu_yy@163.com
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Xian-hui
authors.initials: XH
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Shang
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Ai-rong
authors.initials: AR
authors.lastname: Qian
authors.forename: Xi-ying
authors.initials: XY
authors.lastname: Yao
authors.forename: Zhi-nan
authors.initials: ZN
authors.lastname: Chen
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000302:isolation & purification*; D045744:Cell Line, Tumor; D002846:Chromatography, Affinity; D015179:Colorectal Neoplasms / Q000276:immunology* / Q000473:pathology; D006801:Humans
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm