Cloning and sequence analysis of the self-fertilizing fish Rivulus marmoratus immediate early gene c-fos.
We have cloned the proto-oncogene c-fos from a self-fertilizing fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae) after screening of R. marmoratus lambdaGEM-11 genomic DNA library, and sequenced over 12 kb including all exons, introns and the promoter region. The R. marmoratus c-fos gene consisted of one noncoding exon and four exons with high similarity to those of fugu and mammals. We sequenced approximately 7 kb of the R. marmoratus c-fos gene promoter region to gain a better understanding of the molecular anatomy of the immediate response of this gene upon cellular damage. In the promoter region, R. marmoratus c-fos gene has seven xenobiotic response elements (XREs) and eight metal response elements (MREs) as well as two estradiol (E2), 4 NFkappaB, 2 CarG, 2 prolactin (PRL) motifs and one pit1 site, while the 3'-UTR of this gene contains the estrogen response element (ERE). The seven XRE and eight MRE motifs raise the possibility of its regulation by exposure to environmental pollutants. In this paper, we discuss the gene structure of R. marmoratus c-fos gene and compare its promoter region with those of other organisms' c-fos genes. We propose its potential use in ecotoxicology.
Li
Yan
Y
Department of Biochemistry, College of Medicine, Hanyang University, Seoul 133-791, Republic of Korea.
Kim
Il-Chan
IC
Kim
Young Ja
YJ
Kim
Moon Kyoo
MK
Yoon
Yong-Dal
YD
Lee
Yong-Sung
YS
Lee
Jae-Seong
JS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003532:Cyprinodontiformes / Q000235:genetics*; D017931:DNA Primers; D040461:Gene Components; D016762:Genes, fos / Q000235:genetics*; D015698:Genomic Library; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D020218:Response Elements / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D017931:DNA Primers