Copper-induced oxidative stress in the chlorophycean microalga Chlorella vulgaris: response of the antioxidant system.
A concentration dependent increase in lipid peroxidation, carotenoid content and activity of superoxide dismutase was observed in the green microalga Chlorella vulgaris following copper exposure. In contrast, activities of catalase, ascorbate peroxidase and glutathione reductase, and the cellular GSH, ascorbate and K+ pool depicted a reverse trend. However, a significant rise in intracellular proline content was also evident in copper supplemented cultures. Though this study depicted the malfunction of the major antioxidant system of C. vulgaris under copper stress the test organism was found to survive and grow even at 3.0 microg mL(-1) of Cu treatment (32% growth). Further study is needed to establish the role of proline in metal toxicity regulation.
Mallick
Nirupama
N
Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur-721302, India. nm@agfe.iitkgp.ernet.in
D060387:Ascorbate Peroxidases; D002338:Carotenoids / Q000032:analysis; D002374:Catalase / Q000032:analysis; D002708:Chlorella / Q000187:drug effects* / Q000502:physiology; D003300:Copper / Q000494:pharmacology*; D003470:Culture Media; D005980:Glutathione Reductase / Q000032:analysis; D006861:Hydrogen Peroxide / Q000032:analysis; D015227:Lipid Peroxidation; D018384:Oxidative Stress / Q000187:drug effects*; D010544:Peroxidases / Q000032:analysis; D011392:Proline / Q000502:physiology; D013482:Superoxide Dismutase / Q000032:analysis
D003470:Culture Media; D002338:Carotenoids; D003300:Copper; D011392:Proline; D006861:Hydrogen Peroxide; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D002374:Catalase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase