Enhanced immunodetection of cell-free synthesized erythropoietin under denaturing conditions.
A monoclonal antibody produced by hydridoma cell line, ATCC HB8209, was used to detect and purify erythropoietin synthesized in a cell-free system. The antibody was raised against the N-terminal 20 residues of erythropoietin. It retained anti-erythropoietin activity in 6 M urea in which most of the cell-free synthesized erythropoietin became soluble and gave an enhanced activity of the antibody.
Kang
Taek Jin
TJ
School of Chemical Engineering, College of Engineering, Seoul National University, Seoul 151-742, Korea.
Song
Hui Kyoung
HK
Ahn
Jin Ho
JH
Kim
Tae Wan
TW
Yoon
Yeo Joon
YJ
Choi
Cha Yong
CY
D000818:Animals; D000911:Antibodies, Monoclonal; D002474:Cell-Free System / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004921:Erythropoietin / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D011489:Protein Denaturation
D000911:Antibodies, Monoclonal; D004921:Erythropoietin