[Comparing two kinds of labeled streptavidin biotin methods and selecting the best antigen retrieval method].
OBJECTIVE
To compare two kinds of labeled streptavidin biotin(LsAB) methods and provide the optimal method of antigen retrieval.


METHODS
The alkaline phosphatase(AP) LsAB method and the horseradish peroxidase (HRP) LsAB method were used to stain anti-tryptase in paraffin embedding tissue of patients with chronic obstructive pulmonary disease (COPD) and their staining effects were compared. The antigens were repaired by high pressure cooking, tryptin digestion and microwave, and the repairing effects were compared.


RESULTS
The backgrounds of stained sections were more distinct and the color distinction of nucleus and positive signal were brighter by AP-LsAB, compared with those by HRP-LsAB; the repairing effect of high pressure cooking was better than the repairing effect of tryptin digestion and than that of microwave.


CONCLUSIONS
AP-LsAB and high pressure cooking retrieval were recommended for use in immunohistochemical staining of pulmonary inflammatory tissue.
Zhao
Ying
Y
Department of Laboratory Medicine, West China School of Medicine, Sichuan University, Chengdu 610041, China.
Li
Ping
P
Li
Gui-xing
GX
Deng
Wen-bin
WB
D000469:Alkaline Phosphatase; D000941:Antigens / Q000032:analysis; D001710:Biotin / Q000737:chemistry*; D006735:Horseradish Peroxidase; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008168:Lung / Q000473:pathology; D008872:Microwaves; D011312:Pressure; D029424:Pulmonary Disease, Chronic Obstructive / Q000473:pathology; D019809:Streptavidin / Q000737:chemistry*; D014357:Trypsin
D000941:Antigens; D001710:Biotin; D019809:Streptavidin; D006735:Horseradish Peroxidase; D000469:Alkaline Phosphatase; D014357:Trypsin