High-density lipoproteins contribute to the alpha-1-globulin zone in capillary electrophoresis of human serum proteins.
Alpha-1-antitrypsin (AAT) and alpha-1-acid glycoprotein (AAG) are reported to be the main proteins contributing to the alpha-1-globulin capillary zone electrophoresis (CZE) zone, but the sum (AAT + AAG) showed lower than the alpha-1-globulin. We investigated the role of high-density lipoprotein (HDL), an additional protein migrating in the alpha-1-globulin zone, as a possible cause for such a gap. In a set of 98 sera we measured the alpha-1-globulin with a dedicated clinical capillary electrophoresis system, and AAT, AAG and apolipoprotein A-1 (ApoA) by immunonephelometry. The alpha-1-globulin were consistently higher than the sum (AAT + AAG), by (mean value +/- standard deviation) 1.70 +/- 0.88 g/L in 49 sera with low ApoA, and by 3.59 +/- 0.75 g/L in 49 sera with high ApoA. Corresponding figures in the comparison alpha-1-globulin/(AAT + AAG + ApoA) were reduced to 1.08 +/- 0.77 g/L and 1.67 +/- 0.70 g/L. It is concluded that HDL significantly contribute to the CZE alpha-1-globulin zone, but do not completely explain the differences between the electrophoretic and the immunochemical measurements. However, CZE alpha-1-globulin measurements give information about increases of the two major acute phase proteins comparable to specific protein measurements.
Luraschi
Paola
P
Laboratorio di Chimica Clinica, Ospedale L. Sacco, Via G.B. Grassi 74, I-20157 Milan, Italy.
Pagani
Anna
A
Infusino
Ilenia
I
Moraschinelli
Irene
I
Franzini
Carlo
C
D000510:Alpha-Globulins / Q000737:chemistry*; D016632:Apolipoprotein A-I / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D008075:Lipoproteins, HDL / Q000737:chemistry*; D009961:Orosomucoid / Q000737:chemistry*
D000510:Alpha-Globulins; D016632:Apolipoprotein A-I; D008075:Lipoproteins, HDL; D009961:Orosomucoid; C028184:alpha-1 serum globulin