Antimicrobial activity of isocytisoside and extracts of Aquilegia vulgaris L.
OBJECTIVE
The aim of this study was to analyse the antimicrobial properties extracts of Aquilegia vulgaris, and their principial flavonoid component and to compare the obtained results with the activity of gentamicin and nystatin.


RESULTS
The ethanol, acetone and isopropanol extracts as well as the subextracts isolated from the methanol extract together with the main flavonoid: 4'-methoxy-5,7-dihydroxyflavone 6-C-glucoside (isocytisoside) were obtained from the leaves with stems of Aquilegia vulgaris L. All the extracts were analysed by TLC to confirm flavonoids and phenolic acids occurrence. The antimicrobial activity was tested by the method of series dilutions against different Gram-positive, Gram-negative bacteria and also fungi. The results have shown that the extracts, subextracts and isocytisoside inhibit growth of all studied micro-organisms, revealing the greatest activity against Gram-positive Staphylococcus aureus, Staph. epidermidis and the mould Aspergillus niger.


CONCLUSIONS
The antimicrobial activity of the tested materials it is possibly related to the content of isocytisoside.


CONCLUSIONS
This study has determined new activity of A. vulgaris and suggested the necessity of further studies.
Bylka
W
W
Department of Pharmacognosy, University of Medical Sciences in Poznan, Poznan, Poland. wieslawabylka@o2.pl
Szaufer-Hajdrych
M
M
Matławska
I
I
Goślińska
O
O
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D031884:Aquilegia / Q000737:chemistry*; D005658:Fungi / Q000187:drug effects*; D005839:Gentamicins / Q000494:pharmacology; D005960:Glucosides / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D007529:Isoflavones / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009761:Nystatin / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D005839:Gentamicins; D005960:Glucosides; D007529:Isoflavones; D010936:Plant Extracts; D009761:Nystatin; C111464:isocytisoside