Expression in sugar beet of the introduced cercosporin toxin export (CFP) gene from Cercospora kikuchii, the causative organism of purple seed stain in soybean.
The Cercospora kikuchii cercosporin export gene, CFP, introduced into Beta vulgaris L. by conjugation with Rhizobium radiobacter, was stably maintained during vegetative propagation as verified by PCR using primers specific for the CFP gene. Transcriptional expression of the CFP gene in leaves was determined by RT-PCR using CFP-specific primers. CFP protein was detected using Western analysis with an affinity-purified polypeptide-specifc antibody. Analysis of the relative susceptibility of CFP-transgenic and non-transgenic sugar beet plants is planned but will probably take several years to complete.
Kuykendall
L David
LD
Agricultural Research Service, U.S. Department of Agriculture, Molecular Plant Pathology Lab, Plant Sciences Institute, Beltsville Agricultural Research Center, Beltsville, MD 20705, USA. kuykendl@ba.ars.usda.gov
Upchurch
Robert G
RG
D016960:Agrobacterium tumefaciens / Q000235:genetics; D001203:Ascomycota / Q000235:genetics; D027461:Beta vulgaris / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D015153:Blotting, Western; D004247:DNA / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000235:genetics*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D005838:Genotype; D026901:Membrane Transport Proteins / Q000235:genetics*; D010957:Plasmids / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012313:RNA / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013025:Glycine max / Q000382:microbiology*; D013997:Time Factors; D014158:Transcription, Genetic; D019076:Transgenes
D017931:DNA Primers; D005656:Fungal Proteins; D026901:Membrane Transport Proteins; C400126:cercosporin transporter protein, Cercospora; D012313:RNA; D004247:DNA