title: Molecular typing of enterotoxigenic Staphylococcus aureus isolated from bovine mastitis in Korea.
abstract: One hundred and sixty-six Staphylococcus aureus isolates from mastitic milk samples from different cows on 26 farms were investigated for staphylococcal enterotoxins(SEs) and toxic shock syndrome toxin-1(TSST-1) by polymerase chain reaction(PCR) and reverse passive latex agglutination assay(RPLA). SEs and the TSST-1 gene were detected in thirty-seven isolates based on a multiplex PCR; SEA was detected in 32 isolates, SEB in 3 isolates, SEC in 1 isolate, and SEA and the TSST-1 gene in 1 isolate. Of the 37 enterotoxigenic isolates, thirty-three isolates were enterotoxigenic according to RPLA, where 29 isolates produced SEA, 3 isolates produced SEB, and 1 isolate produced SEC. The enterotoxin-producing S. aureus isolates were further characterized by pulsed-field gel electrophoresis(PFGE). A macrorestriction analysis revealed 11 PFGE patterns. Among the 33 enterotoxigenic S. aureus isolates, 45.4% exhibited the same PFGE pattern I. Accordingly, although the enterotoxin-producing S. aureus isolates from bovine mastitis were genetically diverse, 1 common genotype prevailed on the farms, indicating that PFGE pattern I isolates may be the most disseminated in Korea.
authors.affiliation: National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry, Anyang, Kyonggido, South Korea.
authors.forename: Suk-Kyung
authors.initials: SK
authors.lastname: Lim
authors.forename: Yi-Seok
authors.initials: YS
authors.lastname: Joo
authors.forename: Jin-San
authors.initials: JS
authors.lastname: Moon
authors.forename: Ae-Ri
authors.initials: AR
authors.lastname: Lee
authors.forename: Hyang-Mi
authors.initials: HM
authors.lastname: Nam
authors.forename: Sung-Hwan
authors.initials: SH
authors.lastname: Wee
authors.forename: Hong-Bum
authors.initials: HB
authors.lastname: Koh
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism / Q000382:microbiology*; D003612:Dairying; D016521:Electrophoresis, Gel, Pulsed-Field; D004768:Enterotoxins / Q000235:genetics / Q000378:metabolism*; D007723:Korea; D007841:Latex Fixation Tests; D008414:Mastitis, Bovine / Q000382:microbiology*; D008892:Milk / Q000382:microbiology; D013203:Staphylococcal Infections / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000737:chemistry*; D018089:Superantigens / Q000235:genetics / Q000378:metabolism*
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal