Esterification of vertebrate-like steroids in the eastern oyster (Crassostrea virginica).
The esterification of two model vertebrate steroid hormones - estradiol (E2) and dehidroepiandrosterone (DHEA) - was studied in the oyster Crassostrea virginica. The activity of acyl-CoA:steroid acyltransferase was characterized in microsomal fractions isolated from oyster digestive glands. The apparent Km and Vmax values changed with the fatty acid acyl-CoA used (C20:4, C18:2, C18:1, C16:1, C18:0 or C16:0), and were in the range of 9-17 microM, and 35-74 pmol/min/mg protein for E2, and in the range of 45-120 microM, and 30-182 pmol/min/mg protein for DHEA. Kinetic parameters were also assessed in gonadal tissue. The enzyme saturated at similar concentrations, although conjugation rates were lower than in digestive gland. Preliminary data shows that tributyltin (TBT) in the low microM range (1-50) strongly inhibits E2 and DHEA esterification, the esterification of E2 being more sensitive to inhibition than that of DHEA. Overall, results indicate that apolar conjugation occurs in oysters, in both digestive gland and gonads, at a very similar rate to mammals, suggesting that this is a well conserved conjugation pathway during evolution. Esterification, together with other mechanisms, can modulate endogenous steroid levels in C. virginica, and might be a target for endocrine disrupters, such as TBT.
Janer
Gemma
G
Department of Environmental Chemistry, IIQAB-CSIC, Jordi Girona 18, 08034 Barcelona, Spain. gjgqam@cid.csic.es
Mesia-Vela
Sonia
S
Wintermyer
Margy L
ML
Cooper
Keith R
KR
Kauffman
Fred C
FC
Porte
Cinta
C
D000818:Animals; D015256:Coenzyme A-Transferases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D003687:Dehydroepiandrosterone / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004952:Esters; D004958:Estradiol / Q000378:metabolism*; D006066:Gonads / Q000378:metabolism; D007700:Kinetics; D008861:Microsomes / Q000378:metabolism; D010125:Ostreidae / Q000378:metabolism*; D014220:Trialkyltin Compounds / Q000633:toxicity*
D004952:Esters; D014220:Trialkyltin Compounds; D003687:Dehydroepiandrosterone; D004958:Estradiol; C011559:tributyltin; D015256:Coenzyme A-Transferases