Enhancement of re-closure capacity by the intra-amniotic injection of human embryonic stem cells in surgically induced spinal open neural tube defects in chick embryos.
To evaluate the re-closure promoting capacity of human embryonic stem (hES) cells injected into the amniotic cavity on spinal open neural tube defects (ONTDs) of chick embryos, neural tubes were opened at Hamburger and Hamilton stage 18 or 19 and the embryos were divided into three groups: a control group (no injection), a vehicle group, and a hES cell group (injection of 20,000 hES cells immediately after neural tube incision). On postoperative days 3, 5, and 7, ONTDs were significantly more re-closed in the hES cell group than in the other two groups. hES cells were present at the area in the process of re-closure, and covered ONTDs, but were not found in the re-closed area, suggesting indirect effects rather than cell replacement on the neural tissue.
Lee
Do-Hun
DH
Division of Pediatric Neurosurgery and Laboratory for Fetal Medicine Research, Seoul National University Children's Hospital, 28 Yongon-dong, Chongno-gu, Seoul 110-744, Republic of Korea.
Park
Sepill
S
Kim
Eun Young
EY
Kim
Seung-Ki
SK
Chung
You-Nam
YN
Cho
Byung-Kyu
BK
Lee
Young Jae
YJ
Lim
Jinho
J
Wang
Kyu-Chang
KC
D000650:Amnion; D000818:Animals; D001755:Blastocyst / Q000502:physiology; D002642:Chick Embryo; D005314:Embryonic and Fetal Development; D006801:Humans; D009436:Neural Tube Defects / Q000473:pathology / Q000628:therapy*; D033581:Stem Cell Transplantation; D016019:Survival Analysis