Techniques in protein methylation.
Proteins can be methylated on the side-chain nitrogens of arginine and lysine residues or on carboxy-termini. Protein methylation is a way of subtly changing the primary sequence of a peptide so that it can encode more information. This common posttranslational modification is implicated in the regulation of a variety of processes including protein trafficking, transcription and protein-protein interactions. In this chapter, we will use the arginine methyltransferases to illustrate different approaches that have been developed to assess protein methylation. Both in vivo and in vitro methylation techniques are described, and the use of small molecule inhibitors of protein methylation will be demonstrated.
Lee
Jaeho
J
Deaprtment of Carcinogenesis, Science Park-Research Division, The University of Texas M.D. Anderson Cancer Center, Smithville, TX, USA.
Cheng
Donghang
D
Bedford
Mark T
MT
D048868:Adaptor Proteins, Signal Transducing; D000241:Adenosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D004268:DNA-Binding Proteins; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005982:Glutathione Transferase / Q000737:chemistry / Q000235:genetics; D006367:HeLa Cells; D006657:Histones / Q000737:chemistry / Q000187:drug effects; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008745:Methylation / Q000187:drug effects; D008780:Methyltransferases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D039122:Poly(A)-Binding Protein I / Q000378:metabolism; D011233:Precipitin Tests / Q000379:methods; D011499:Protein Processing, Post-Translational; D011484:Protein-Arginine N-Methyltransferases; D016601:RNA-Binding Proteins / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification; D012436:S-Adenosylmethionine / Q000737:chemistry / Q000494:pharmacology; D015398:Signal Transduction; D013379:Substrate Specificity
D048868:Adaptor Proteins, Signal Transducing; D004268:DNA-Binding Proteins; D004791:Enzyme Inhibitors; D006657:Histones; D047908:Intracellular Signaling Peptides and Proteins; C489559:KHDRBS1 protein, human; D039122:Poly(A)-Binding Protein I; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; C027579:periodate-oxidized adenosine; D012436:S-Adenosylmethionine; D008780:Methyltransferases; C077013:PRMT2 protein, human; D011484:Protein-Arginine N-Methyltransferases; D005982:Glutathione Transferase; D000241:Adenosine; C006235:sinefungin