Cytogenetic markers, DNA single-strand breaks, urinary metabolites, and DNA repair rates in styrene-exposed lamination workers.
The effect of occupational exposure to styrene on frequencies of chromosomal aberrations and binucleated cells with micronuclei and on single-strand break levels in peripheral blood lymphocytes was studied in 86 reinforced plastic workers and 42 control individuals (including 16 maintenance workers with intermittent, low-dose exposure). In these individuals, the irradiation-specific DNA repair rates and the repair rates of 8-oxoguanines were investigated. We assessed the exposure by measuring the concentrations of styrene in air and in blood and of mandelic acid, phenylglyoxylic acid, 4-vinyl phenol conjugates and regioisomeric phenyl hydroxyethyl mercapturic acids in urine. All these parameters correlated with one another. No clear relationship was found between the styrene exposure and the frequencies of chromosomal aberrations. Binucleated cells with micronuclei were moderately related to the parameters of styrene exposure. We found a negative correlation between all exposure parameters and single-strand breaks. The positive correlation between exposure parameters and DNA repair rates suggests that particular DNA repair pathways may be induced by styrene exposure.
Vodicka
Pavel
P
Institute of Experimental Medicine, Academy of Science of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic. pvodicka@biomed.cas.cz
Tuimala
Jarno
J
Stetina
Rudolf
R
Kumar
Rajiv
R
Manini
Paola
P
Naccarati
Alessio
A
Maestri
Luciano
L
Vodickova
Ludmila
L
Kuricova
Miroslava
M
Järventaus
Hilkka
H
Majvaldova
Zuzana
Z
Hirvonen
Ari
A
Imbriani
Marcello
M
Mutti
Antonio
A
Migliore
Lucia
L
Norppa
Hannu
H
Hemminki
Kari
K
D000328:Adult; D016022:Case-Control Studies; D002617:Chemical Industry; D002869:Chromosome Aberrations; D004249:DNA Damage; D004260:DNA Repair; D005260:Female; D006801:Humans; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D016273:Occupational Exposure; D010969:Plastics; D020058:Styrene / Q000506:poisoning*
D010969:Plastics; D020058:Styrene