Identification of a novel allele HLA-A*1113 in a Japanese donor.
Anew HLA-A*11 allele, A*1113, was identified in a healthy Japanese female. She was typed as HLA-A11?, A2, B46, B67, Cw1, Cw7 (Bw6) with unusual serological reactivity of A11, suggesting possible presence of a new A*11 allele. The novel A*1113 allele was identified by haplotypic group-specific allele amplification using A*11 allele-specific primer pairs and sequence-based typing. The A*1113 allele differs from A*11011 by one nucleotide substitution in exon 3 at position 503 (A --> G) which causes an amino acid change in the alfa2 domain at residue 144 (lysine : K --> arginine : R), thus resulting in the unusual serological reactivity.
Takahashi
D
D
Hokkaido Red Cross Blood Center, Sapporo, Japan.
Miyazaki
T
T
Araseki
M
M
Sekimoto
T
T
Sato
S
S
Kato
T
T
Ikeda
H
H
D000483:Alleles; D019943:Amino Acid Substitution; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D005260:Female; D015234:HLA-A Antigens / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D015234:HLA-A Antigens; D004247:DNA