[CCND1 gene amplification in the adenoid cystic carcinoma of the minor salivary glands].
OBJECTIVE
Adenoid cystic carcinoma (ACC) is a tumour of epithelial origin that represents the most common malignant neoplasm of the minor salivary glands. However, little is known about the genes involved in the development and progression of this tumour. Cyclin D1 gene (CCND1) plays a key role in the control of the cell cycle, and its amplification is described in numerous cancers. The aim of this study is to determine the amplification of the CCND1 gene in the ACC of the minor salivary glands.


METHODS
A retrospective study was performed on 12 patients with ACC of the head and neck. The amplification of the CCND1 was determined using multiple PCR.


RESULTS
Amplification of the CCND1 was found in 4 patients (33.3%). No correlation was found between CCND1 amplification and clinicopathological parameters, although disease-free survival was diminished in patients with amplification.


CONCLUSIONS
Our study demonstrates for the first time the amplification of the CCND1 gene in ACC. We have found an amplification rate similar to others neoplasms. CCND1 amplification seems to be associated with a poorer prognosis in these tumours, although this needs to be confirmed in larger studies.
Sequeiros Santiago
G
G
Servicio de ORL, Hospital Central de Asturias, Oviedo. gsequeiros_orl@hotmail.com
Rodrigo Tapia
J P
JP
García-Carracedo
D
D
García Pedrero
J
J
Suárez Nieto
C
C
González Meana
M V
MV
D000328:Adult; D000368:Aged; D003528:Carcinoma, Adenoid Cystic / Q000235:genetics* / Q000473:pathology*; D019938:Cyclin D1 / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D006801:Humans; D008297:Male; D008875:Middle Aged; D012468:Salivary Gland Neoplasms / Q000235:genetics* / Q000473:pathology*
D019938:Cyclin D1