title: Polymorphism in the N-acetyltransferase 1 alleles NAT1*10 and NAT1*14A and cytological gradings of exfoliated urothelial cells in benzidine-exposed Chinese workers: discussion of ethnic differences.
abstract: N-acetyltransferase 1 (NAT1) modifies bladder cancer risk in European populations exposed to aromatic amines in cigarette smoke. The present study was performed to investigate a possible association between NAT1*10 and NAT1*14A genotypes and bladder cancer risk in benzidine-exposed Chinese workers. Based on the cytological gradings of exfoliated urothelial cells according to Papanicolaou, an exposed research cohort was stratified into subgroups. An allele-specific PCR-based procedure was used to detect the polymorphism in the polyadenylation signal at the locus NAT1 T(1088)A. A nested PCR-RFLP procedure was conducted to differentiate NAT1*14A (T(1088)A, C(1095)A, and G(560)A) from NAT1*10 (T(1088)A, C(1095)A). No significantly different frequencies of homozygous and heterozygous NAT1*10 alleles were found among the subgroups with (i) gradings according to Papanicolaou < or = II (18.3 and 40.2%, respectively), (ii) higher gradings according to Papanicolaou (> II; 28.0 and 34.1%, respectively), and (iii) with bladder cancer (26.3 and 34.2%, respectively). The present data show that NAT1*10 neither displayed an association with an elevated grading of urothelial cells nor a clear impact on the risk for bladder cancer in benzidine-exposed Chinese workers. Discrepancies with the findings in European populations could point to ethnic differences in the disposition of aromatic amines.
authors.affiliation: Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 225 Chongqing Rd. (S.), 200025 Shanghai, China.
authors.forename: Wei Chao
authors.initials: WC
authors.lastname: Guo
authors.forename: Guo Fang
authors.initials: GF
authors.lastname: Lin
authors.forename: Ji Gang
authors.initials: JG
authors.lastname: Chen
authors.forename: Klaus
authors.initials: K
authors.lastname: Golka
authors.forename: Jian Hua
authors.initials: JH
authors.lastname: Shen
mesh_terms: D000328:Adult; D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D001560:Benzidines / Q000009:adverse effects*; D002273:Carcinogens / Q000009:adverse effects*; D002295:Carcinoma, Transitional Cell / Q000139:chemically induced / Q000453:epidemiology / Q000235:genetics; D002681:China / Q000453:epidemiology; D004273:DNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D005006:Ethnicity; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008297:Male; D016273:Occupational Exposure; D065006:Papanicolaou Test; D011110:Polymorphism, Genetic / Q000235:genetics*; D012907:Smoking / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced / Q000453:epidemiology / Q000235:genetics; D019459:Urothelium / Q000166:cytology*; D014626:Vaginal Smears
chemical_list: D001560:Benzidines; D002273:Carcinogens; D004273:DNA, Neoplasm; D007527:Isoenzymes; D001191:Arylamine N-Acetyltransferase; C089384:N-acetyltransferase 1