[Transfection of human endostatin gene with lipofectamin and the expression of hES protein in Tca8113 cell].
OBJECTIVE
The purpose of this study was to establish transfergeneic Tca8113 cell and evaluate the expression of human endostatin (hES) gene in the cell colone in vitro.


METHODS
To transfect hES gene into Tca8113 cells, lipofectamin was complexed with plasmid encoding hES gene, and blasticidin S antibiotic was adopted to select Tca8113--hES cell clone. Immunohistochemistry S-P method was adopted to detect the expression of hES in the transfergenic Tca8113 cell in vitro.


RESULTS
Transfected by hES, the transfergenic Tca8113 cells could grow and proliferate in RPMI--1640 culture medium containing blasticidin S antibiotic. The expression rate of hES reached 100%.


CONCLUSIONS
hES gene can express in hES-transfected Tca8113 cell in vitro.
Pan
Chao-bin
CB
Dept. of Oral and Maxillofacial Surgery, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China.
Huang
Hong-zhang
HZ
Wang
Jian-guang
JG
Hou
Jin-song
JS
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002455:Cell Division; D003001:Cloning, Molecular; D043169:Endostatins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008055:Lipids / Q000494:pharmacology; D014062:Tongue Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D014162:Transfection; D014407:Tumor Cells, Cultured
D043169:Endostatins; D008055:Lipids; C086724:Lipofectamine