New amperometric biosensors based on diamond paste for the assay of L- and D-pipecolic acids in serum samples.
Monocrystalline natural diamond, L-amino acid oxidase (L-AAOD), D-amino acid oxidase (D-AAOD), and paraffin oil were used for the design of the modified diamond paste. The technique used for the direct voltammetric assay was differential pulse voltammetry (DPV) with applied potential pulse amplitude of 25 mV vs. Ag/AgCl. Using the new amperometric biosensors L-pipecolic acid (L-PA) and D-pipecolic acid (D-PA) were determined reliably from serum samples at 700 and 200 mV vs. Ag/AgCl, respectively, with low limits of detection.
Stefan
Raluca-Ioana
RI
Department of Chemistry, University of Pretoria, Pretoria, South Africa. raluca-stefan@chem.up.ac.za
Nejem
R'afat Mahmoud
RM
van Staden
Jacobus F
JF
Aboul-Enein
Hassan Y
HY
D000594:Amino Acid Oxidoreductases / Q000737:chemistry*; D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D018130:Diamond / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D006801:Humans; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000175:diagnosis; D008107:Liver Diseases / Q000175:diagnosis; D009821:Oils / Q000737:chemistry; D009824:Ointments / Q000737:chemistry; D010232:Paraffin; D010875:Pipecolic Acids / Q000032:analysis* / Q000097:blood; D012680:Sensitivity and Specificity; D044967:Serum / Q000737:chemistry*; D013237:Stereoisomerism
D015415:Biomarkers; D009821:Oils; D009824:Ointments; D010875:Pipecolic Acids; D018130:Diamond; D010232:Paraffin; C015418:paraffin oils; D000594:Amino Acid Oxidoreductases; C031345:pipecolic acid