Amphetamines and 3,4-methylendioxymetamphetamine (MDMA): evaluation of KIMS (kinetic interaction of microparticles in solution) assay at two cut-off levels.
Two screening methods for the assay of amphetamines and their derivatives have been applied to the same analytical instrument for their evaluation. In addition to an assay at a cut-off of 1000 microg/l, a new specific reagent was evaluated for an ultra-sensitive assay of amphetamines and 3,4-methylendioxymetamphetamine with a cut-off of 300 microg/l. The assay confirmation was performed using high-performance liquid chromatography and gas chromatography/ mass spectrometry techniques. The results were positive for both screening methods, confirming the efficacy of two simultaneous methods with different cut-off levels.
Marchioro
Lucio
L
Department of Laboratory Medicine, University-Hospital of Padova, Padova, Italy.
Bassetto
Flavia
F
Dall'Olio
Giuliano
G
Tedeschi
Luciano
L
Castagna
Franca
F
Plebani
Mario
M
D015104:3,4-Methylenedioxyamphetamine / Q000652:urine; D000293:Adolescent; D000328:Adult; D000662:Amphetamines / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D019963:Clinical Chemistry Tests / Q000379:methods* / Q000706:statistics & numerical data; D017248:Enzyme Multiplied Immunoassay Technique / Q000706:statistics & numerical data; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000706:statistics & numerical data; D008297:Male; D008694:Methamphetamine / Q000652:urine; D008875:Middle Aged; D018817:N-Methyl-3,4-methylenedioxyamphetamine / Q000652:urine*; D049329:Nanostructures; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D019966:Substance-Related Disorders / Q000175:diagnosis / Q000652:urine
D000662:Amphetamines; D008694:Methamphetamine; D015104:3,4-Methylenedioxyamphetamine; D018817:N-Methyl-3,4-methylenedioxyamphetamine