Genetic regulation of mouse glycosylphosphatidylinositol-phospholipase D.
Glycosylphosphatidylinositol phospholipase D (GPI-PLD) has been proposed to be responsible for cleaving membrane-associated glycosylphosphatidyl inositol (GPI) molecules to generate inositol phosphoglycan (IPGs), which have growth factor-mimetic properties. We have cloned the mouse liver GPI-PLD cDNA, which has a sequence that differs from that previously isolated from a mouse glucagonoma cell library. Using a highly specific and very sensitive RNase protection assay, we found that the GPI-PLD expressed in adult/post-natal brain, antrum and insulin-producing cells is identical to that isolated from liver. The expression of mouse GPI-PLD in liver shows a complex genetic regulation with a mouse strain-specific variation. In addition, GPI-PLD mRNA levels were higher in 4-week old animals compared to older animals, and the GPI-PLD mRNA levels increased in mice that developed insulin dependent type 1 diabetes spontaneously. This suggests that the expression of liver GPI-PLD in mice is highly regulated.
Flores-Borja
Fabian
F
Department of Immunology and Molecular Pathology, University College London, 46 Cleveland street, London W1T 4JF, UK.
Kieszkievicz
Julius
J
Church
Vicki
V
Francis-West
Phillipa H
PH
Schofield
Julian
J
Rademacher
Thomas W
TW
Lund
Torben
T
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D018076:DNA, Complementary; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D005935:Glucagonoma / Q000378:metabolism / Q000473:pathology; D007328:Insulin / Q000378:metabolism; D008099:Liver / Q000196:embryology / Q000201:enzymology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD / Q000196:embryology; D008969:Molecular Sequence Data; D010739:Phospholipase D / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D007328:Insulin; D012333:RNA, Messenger; D012260:Ribonucleases; D010739:Phospholipase D; C065017:glycoprotein phospholipase D