title: [Proteomic analysis of proteins involved in the renal phenotype in renovascular hypertension].
abstract: Renovascular hypertension is characterised by stenosis of the renal artery and high plasma renin levels due to the recruitment of renin-producing cells along the afferent arterioles. This increase in myoepithelioid cells is mainly a result of the differentiation of existing smooth muscle cells with acquisition of a secretory phenotype. To understand the molecular mechanisms involved in this recruitment, we used the model of renovascular hypertension known as the two-kidney, one-clip model in the Lewis rat. Renal arterioles were isolated using magnetised iron suspension. Differential proteomic analysis was performed using two-dimensional electrophoresis gel followed by mass spectrometry for identification. The most striking protein revealed by proteomics is troponin T, which is down-regulated in the afferent arterioles of the clipped kidney. Confocal microscopy showed that troponin T is specific to the smooth muscle phenotype and absent in the myoepithelioid phenotype.
authors.affiliation: INSERM U508, Institut Pasteur de Lille, Lille, France. florence.pinet@pasteur-lille.fr
authors.forename: Florence
authors.initials: F
authors.lastname: Pinet
authors.forename: Florence
authors.initials: F
authors.lastname: Poirier
authors.forename: Sébastien
authors.initials: S
authors.lastname: Fuchs
authors.forename: Pierre-Louis
authors.initials: PL
authors.lastname: Tharaux
authors.forename: Michel
authors.initials: M
authors.lastname: Caron
authors.forename: Pierre
authors.initials: P
authors.lastname: Corvol
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Michel
authors.forename: Raymonde
authors.initials: R
authors.lastname: Joubert-Caron
mesh_terms: D000818:Animals; D001160:Arterioles / Q000378:metabolism / Q000473:pathology; D004591:Electrophoresis, Polyacrylamide Gel; D006978:Hypertension, Renovascular / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D013058:Mass Spectrometry; D010641:Phenotype; D011506:Proteins / Q000235:genetics*; D040901:Proteomics; D051381:Rats; D011917:Rats, Inbred Lew; D012079:Renal Circulation
chemical_list: D011506:Proteins