Optimization of procedures for isolation of mycobacteria from soil and water samples obtained in northern India.
For isolation of environmental mycobacteria, a decontamination procedure has been standardized by which treatment with 3% sodium dodecyl sulfate plus 4% NaOH (15 and 30 min for rapid and slow growers, respectively) is followed by incubation with 2% cetrimide (5 and 15 min for fast- and slow-growing mycobacteria, respectively); this procedure was found to completely eliminate contamination with other organisms and resulted in the isolation of only mycobacteria.
Parashar
Deepti
D
Central JALMA Institute for Leprosy and Other Mycobacterial Diseases, Indian Council of Medical Research, Tajganj, Agra 28201, India.
Chauhan
D S
DS
Sharma
V D
VD
Chauhan
Aradhana
A
Chauhan
S V S
SV
Katoch
V M
VM
D001431:Bacteriological Techniques; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology; D004203:Disinfection / Q000379:methods; D007194:India; D009170:Nontuberculous Mycobacteria / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D012972:Sodium Hydroxide / Q000494:pharmacology; D012988:Soil Microbiology; D014871:Water Microbiology
D002593:Cetrimonium Compounds; D012967:Sodium Dodecyl Sulfate; D012972:Sodium Hydroxide; D000077286:Cetrimonium