Single-molecule spectroscopy for nucleic acid analysis: a new approach for disease detection and genomic analysis.
Recently developed single-molecule spectroscopy (SMS) permits the analysis of fluorescent mixtures one molecule at a time. SMS methods provide the means to make rapid measurements on small, complex samples without the need for separations and target amplification enabling a new class of ultrasensitive nucleic acid assays. Here we give a brief overview of the current state of the art of SMS nucleic acid analysis and discuss ongoing work in our laboratory on two-color single-molecule fluorescence detection of specific nucleic acid sequences. In the future, two-color SMS nucleic acid assays will be used for a variety of applications including: gene expression analysis, disease detection and genomics.
Goodwin
Peter M
PM
Los Alamos National Laboratory, Bioscience Division, New Mexico 87545, USA. pmg@lanl.gov
Nolan
Rhiannon L
RL
Cai
Hong
H
D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation / Q000379:methods*; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods*; D005820:Genetic Testing / Q000379:methods*; D023281:Genomics / Q000295:instrumentation / Q000379:methods; D006239:Haplotypes; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods; D008967:Molecular Biology / Q000295:instrumentation / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods
D004247:DNA