title: Reactive oxygen species (ROS) generation inhibited by aporphine and phenanthrene alkaloids semi-synthesized from natural boldine.
abstract: Four phenanthrene and one aporphine alkaloids semi-synthesized from boldine were evaluated for their inhibitory effect on reactive oxygen species (ROS) generation. ROS generation by neutrophils stimulated with N-formyl-methionyl-leucyl-phenylalanine was inhibited in a concentration dependent manner. Alkaloids exerted similar inhibitory effect in the hypoxanthine-xanthine oxidase system than in stimulated neutrophils, which could be attributed to a direct ROS scavenging activity. None of the alkaloids assayed had any effect on xanthine oxidase activity. Therefore the synthesized alkaloids might constitute an alternative therapy in inflammation disorders in which ROS generation is involved.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Valencia, Avda. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain.
authors.forename: Lara
authors.initials: L
authors.lastname: Milián
authors.forename: Rossana
authors.initials: R
authors.lastname: Estellés
authors.forename: Belén
authors.initials: B
authors.lastname: Abarca
authors.forename: Rafael
authors.initials: R
authors.lastname: Ballesteros
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Sanz
authors.forename: María Amparo
authors.initials: MA
authors.lastname: Blázquez
mesh_terms: D000470:Alkaloids / Q000138:chemical synthesis / Q000494:pharmacology*; D001060:Aporphines / Q000138:chemical synthesis / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D010616:Phenanthrenes / Q000138:chemical synthesis / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D000470:Alkaloids; D001060:Aporphines; D010616:Phenanthrenes; D017382:Reactive Oxygen Species; C081279:aporphine; C011686:boldine