The generation of high biomass from chlororespiring bacteria using a continuous fed-batch bioreactor.
A continuous fed-batch reactor system was developed to rapidly obtain dense chlororespiring cultures of Anaeromyxobacter dehalogenans strain 2CP-C. A syringe pump continuously delivered concentrated 2,6-dichlorophenol (50-150 mM) to an anaerobic reactor vessel at a rate that sustained linear growth but prevented the substrate toxicity of chlorophenol. Dechlorination was not significantly inhibited by end product phenol up to 8 mM. A cell density of 76.8 mg protein l(-1) was obtained in 24 h. Specific growth rates averaged 0.033 h(-1)at 50% substrate limitation, which was in agreement with the maximum specific growth rate of 0.068 h(-1). This reactor system provides an efficient, cost-effective, and convenient method to rapidly obtain dense dechlorinating biomass and is promising to accelerate investigations of enzymes involved in chlororespiration.
He
Qiang
Q
Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801-2352, USA.
Sanford
Robert A
RA
D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000032:analysis; D001673:Biodegradation, Environmental; D018533:Biomass; D019149:Bioreactors; D017606:Chlorine Compounds / Q000378:metabolism*; D002733:Chlorophenols / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism; D009231:Myxococcales / Q000254:growth & development* / Q000378:metabolism*; D019800:Phenol / Q000378:metabolism
D001426:Bacterial Proteins; D017606:Chlorine Compounds; D002733:Chlorophenols; D004798:Enzymes; D019800:Phenol; C032729:2,6-dichlorophenol