Nitroxide-labeled guanine as an ESR spin probe for structural study of DNA.
A guanine derivative with a covalently linked nitroxide spin label has been devised. The spin label was incorporated into oligodeoxynucleotides by post-synthetic modification. The local environment of a variety of G-rich DNA is detectable by ESR using this spin probe.
Okamoto
Akimitsu
A
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University and SORST, Japan Science and Technology Corporation, Kyoto 615-8510, Japan.
Inasaki
Takeshi
T
Saito
Isao
I
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004577:Electron Probe Microanalysis; D004578:Electron Spin Resonance Spectroscopy; D006147:Guanine / Q000737:chemistry*; D009589:Nitrogen Oxides / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D013113:Spin Labels
D009589:Nitrogen Oxides; D013113:Spin Labels; D006147:Guanine; D004247:DNA