Multiplex PCR assay for rapid detection and genotyping of Helicobacter pylori directly from biopsy specimens.
We developed and evaluated a simple, novel multiplex PCR assay for rapid detection of Helicobacter pylori infection and for the determination of vacA and cagA genotypes directly from gastric biopsy specimens. This assay did not require culturing of strains or extraction of DNA from biopsy samples. This multiplex PCR assay would be of particularly great value for laboratories in developing countries.
Chattopadhyay
Santanu
S
National Institute of Cholera and Enteric Diseases, Calcutta 700010, India.
Patra
Rajashree
R
Ramamurthy
T
T
Chowdhury
Abhijit
A
Santra
Amal
A
Dhali
G K
GK
Bhattacharya
S K
SK
Berg
Douglas E
DE
Nair
G Balakrish
GB
Mukhopadhyay
Asish K
AK
D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001706:Biopsy; D005838:Genotype; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori