Acute and chronic changes of vascular endothelial growth factor (VEGF) in induced sputum of toluene diisocyanate (TDI)-induced asthma patients.
Vascular endothelial growth factor (VEGF) is a multi-functional cytokine involved in inflammation, repair and angiogenesis in asthmatic airway. This study aimed to evaluate the role of VEGF in immediate bronchoconstriction induced by TDI inhalation, and in chronic TDI-asthma patients. 11 newly diagnosed TDI-asthma patients (group I), 12 chronic TDI-asthma patients with persistent asthma symptoms followed for >4 yr and 15 unexposed healthy controls were enrolled. In group I, induced sputum and serum were collected before and 7 hr after placebo- and TDI-bronchoprovocation test (BPT). In group II, induced sputum and serum were collected every 2 yr. VEGF levels were measured by ELISA. There were no significant differences in sputum and serum VEGF levels between patients and controls. Before and after placebo and TDI-BPT, no significant changes were noted in sputum and serum VEGF levels of group I. In group II patients, sputum VEGF showed variable changes at 1-yr, then decreased significantly at 2-yr (p<0.05), while serum VEGF showed variable changes at 2-yr, which decreased significantly at 4-yr (p<0.05). These results suggest that VEGF may play a minor role in immediate bronchoconstriction after TDI-BPT. In chronic TDI-asthma, VEGF may be involved to 2 yr after the diagnosis and the contribution may decrease after then.
Choi
Jeong-Hee
JH
Department of Allergy and Rheumatology, Ajou University School of Medicine, Suwon, Korea.
Suh
Yu-Jin
YJ
Lee
Soo-Keol
SK
Suh
Chang-Hee
CH
Nahm
Dong-Ho
DH
Park
Hae-Sim
HS
D000328:Adult; D001249:Asthma / Q000139:chemically induced* / Q000378:metabolism*; D001980:Bronchi / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D015444:Exercise; D006801:Humans; D016210:Methacholine Chloride / Q000494:pharmacology; D008875:Middle Aged; D010919:Placebos; D013183:Sputum / Q000378:metabolism*; D013997:Time Factors; D014051:Toluene 2,4-Diisocyanate / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000378:metabolism
D010919:Placebos; D042461:Vascular Endothelial Growth Factor A; D016210:Methacholine Chloride; D014051:Toluene 2,4-Diisocyanate