title: Nonlinear matching measure for the analysis of on-off type DNA microarray images.
abstract: We propose a new nonlinear matching measure for automatic analysis of the on-off type DNA microarray images in which the hybridized spots are detected by a template-matching method. The proposed measure is obtained by binary thresholding over the entire template region and taking the number of white pixels inside the spotted area. This measure is compared with the normalized covariance method in terms of classifying the ability to successfully locate markers. The proposed measure was evaluated for scanned images of human papillomavirus (HPV) DNA microarrays where locating markers is a critical issue because of the small number of spots. The targeting spots of HPV DNA chips are designed for genotyping twenty-two types of the human papillomavirus. The proposed measure is proven to give a more discriminative response, reducing the missed cases of successful marker location. The locating accuracy of the proposed method is also shown to have the same performance as that of the normalized covariance.
authors.affiliation: Seoul National University, Graduate School, Interdisciplinary Program in Biomedical Engineering Major, Seoul 110-744, Korea. mhr@bio.bmelab.co.kr
authors.forename: Munho
authors.initials: M
authors.lastname: Ryu
authors.forename: Jong
authors.initials: J
authors.lastname: Dae Kim
authors.forename: Byoung
authors.initials: B
authors.lastname: Goo Min
authors.forename: Myung-Geol
authors.initials: MG
authors.lastname: Pang
authors.forename: Jongwon
authors.initials: J
authors.lastname: Kim
mesh_terms: D000465:Algorithms; D015344:DNA Probes, HPV; D005820:Genetic Testing / Q000379:methods; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D017711:Nonlinear Dynamics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D010363:Pattern Recognition, Automated; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D015344:DNA Probes, HPV