Detection of Grb-2-related adaptor protein gene (GRAP) and peptide molecule in salivary glands of MRL/lpr mice and patients with Sjögren's syndrome.
The pathogenesis of Sjögren's syndrome (SS) is poorly understood. In this study we used an in-house mouse spleen cDNA microarray to analyse genes in spleens from MRL/lpr (an SS mouse model) mice. We have previously demonstrated that GRAP genes were up-regulated in salivary glands of the same mice. The microarray analysis showed that seven out of 2304 genes were highly expressed in spleens from the MRL/lpr mice, one of which was the GRAP gene. In other words, the GRAP gene is highly expressed in the salivary glands and spleen of MRL/lpr mice. We also carried out immunohistochemical studies. Mouse and human Grb-2-related adaptor protein (GRAP) antigens were expressed on ductal cells and infiltrating lymphocytes in salivary glands of MRL/lpr mice and SS patients, but only weakly in controls (MRL/+ mice and individuals with salivary cysts). These results suggest that the GRAP gene might have a role in the pathogenesis of SS.
Shiraiwa
H
H
First Department of Internal Medicine, Nihon University School of Medicine, Nerima Hikarigaoka Nihon University Hospital, Tokyo, Japan.
Takei
M
M
Yoshikawa
T
T
Azuma
T
T
Kato
M
M
Mitamura
K
K
Ueki
T
T
Kida
A
A
Horie
T
T
Seki
N
N
Sawada
S
S
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D019463:Mice, Inbred MRL lpr; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D012469:Salivary Glands / Q000166:cytology / Q000473:pathology / Q000502:physiology*; D012859:Sjogren's Syndrome / Q000378:metabolism*; D013154:Spleen / Q000502:physiology
D048868:Adaptor Proteins, Signal Transducing; C409587:GRAP2 protein, human; C510602:Grap protein, human; C498726:Mona protein, mouse; D010455:Peptides