A complex rearrangement of chromosome 7 in human astrocytoma.
Chromosome 7 is a frequent site of cytogenetic aberrations in human astrocytomas. One region that is often targeted in human astrocytomas is on 7p. The U251 human glioblastoma cell line has a region of gain of genetic material on 7p similar to that seen in human astrocytomas. We used several cytogenetic techniques to study chromosome 7 in U251 cells and identified a complex rearrangement that accounts for gain of chromosome 7 genetic material in the cell line. The characteristic rearrangement suggests a mechanism leading to 7p gain in primary grade IV astrocytomas.
Misra
Anjan
A
Division of Neurology, Barrow Neurological Institute, Phoenix, AZ, USA. amisra@cc.ucsf.edu
Pellarin
Malgorzata
M
Shapiro
Joan R
JR
Feuerstein
Burt G
BG
D001254:Astrocytoma / Q000235:genetics*; D001932:Brain Neoplasms / Q000235:genetics*; D002869:Chromosome Aberrations; D002897:Chromosomes, Human, Pair 7; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping