Simultaneous PCR detection of ica cluster and methicillin and mupirocin resistance genes in catheter-isolated Staphylococcus.
Recent data show that more than 50% of catheter-associated bloodstream infections are caused by staphylococci. Staphylococcal infections produced by intercellular-adhesion cluster (ica) carriers can be even more problematic due to the presence of methicillin and mupirocin resistance genes. In the present study, a multiplex PCR protocol that allows the simultaneous identification of staphylococci and detection of both the ica and methicillin and/or mupirocin resistance genes was developed. Furthermore, the method allows differential detection of the ica locus from Staphylococcus aureus and Staphylococcus epidermidis.
Martín-López
Juana V
JV
Molecular Biology Laboratory, Research Unit, Nuestra Señora de Candelaria University Hospital, Santa Cruz de Tenerife, 38010 Santa Cruz de Tenerife, Spain.
Díez-Gil
Oscar
O
Morales
Manuel
M
Batista
Ninivé
N
Villar
Jesús
J
Claverie-Martín
Félix
F
Méndez-Alvarez
Sebastián
S
D001483:Base Sequence; D002408:Catheters, Indwelling; D017931:DNA Primers; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics*; D016712:Mupirocin / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods; D013210:Staphylococcus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification
D017931:DNA Primers; D016712:Mupirocin