title: [Expression of substance P receptor positive cells in the Corti's organ with acute middle ear infection in guinea pigs].
abstract: OBJECTIVE
  To investigate the expression of substance P receptor(SPR), SPR positive cells in the Corti's organ with acute middle ear infection in guinea pigs using the polyclonal antibody of SPR.
  
  
  METHODS
  Twelve healthy guinea pigs were employed in the experiment. After general anesthesia, by injecting 1 x 10(8)/L staphylococcosis aureus into the middle cavity of right ear with the left ear serving as control, the acute middle ear infection model was established. Then three days later, immunohistochemical staining of SPR was performed in the Cochlear base membrane preparation.
  
  
  RESULTS
  Microscopic examination of whole cochlear preparation revealed a number of SPR positive cells expression in the cochlear base membrane, these labeled cells usually did not exist in normal cochlear tissue. Obvious difference in morphology and distribution could be identified with inner hair cells, outer hair cells vascular endothelial cell and spiral ganglion neurons. Labeled SP receptor positive cells were similar to "neurons", scattering distributed in the free margin of cochlear base membrane, with larger size and multiple projections which was 6-12 times than the erythrocyte. There were the vesicle and granular substances in the cytoplasm of the labeled cells.
  
  
  CONCLUSIONS
  Acute middle ear infection could induce the expression of SP receptor positive nonspecific cells in the Corti's organ of guinea pigs. These cells did not exist in the normal base membrane and might participate in initiating or inducing the immune response of inner ear.
authors.affiliation: Center of Otorhinolaryngology of Chinese PLA, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. qiujh@fmmu.edu.cn
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Qiu
authors.forename: Shun-li
authors.initials: SL
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Su
authors.forename: Li
authors.initials: L
authors.lastname: Qiao
mesh_terms: D000208:Acute Disease; D000818:Animals; D001485:Basement Membrane / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D009925:Organ of Corti / Q000378:metabolism*; D010033:Otitis Media / Q000378:metabolism* / Q000382:microbiology; D011817:Rabbits; D018040:Receptors, Neurokinin-1 / Q000378:metabolism*; D013203:Staphylococcal Infections / Q000378:metabolism; D013373:Substance P / Q000378:metabolism
chemical_list: D018040:Receptors, Neurokinin-1; D013373:Substance P