title: Induction of cell differentiation in human leukemia U-937 cells by 5-oxygenated-6,7-methylenedioxycoumarins from Pterocaulon polystachyum.
abstract: The present study focused on the effect of a series of extracts and two 5,6,7-trioxygenated coumarins isolated from Pterocaulon polystachyum on the proliferation and differentiation of human promonocytic U-937 cells. The petroleum ether extract was the only extract that significantly reduced cell proliferation and induced cell differentiation. Treatment with pure 5-methoxy-6,7-methylenedioxycoumarin (C1) and 5-(3-methyl-2-butenyloxy)-6,7-methylenedioxycoumarin (C2), present in the petroleum ether extract, showed a time and concentration-dependent inhibition on cell proliferation. In addition, the coumarin derivatives were also able to induce CD88 functionality and NBT reduction, markers of monocytic cell differentiation. These results suggest that C1 and C2 might have a potential therapeutic role in the management of leukemia.
authors.affiliation: Laboratorio de Radioisótopos, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956 PB, 1113. Capital Federal, Buenos Aires, Argentina.
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Riveiro
authors.forename: Carina
authors.initials: C
authors.lastname: Shayo
authors.forename: Federico
authors.initials: F
authors.lastname: Monczor
authors.forename: Natalia
authors.initials: N
authors.lastname: Fernández
authors.forename: Alberto
authors.initials: A
authors.lastname: Baldi
authors.forename: Norbert
authors.initials: N
authors.lastname: De Kimpe
authors.forename: Javier
authors.initials: J
authors.lastname: Rossi
authors.forename: Silvia
authors.initials: S
authors.lastname: Debenedetti
authors.forename: Carlos
authors.initials: C
authors.lastname: Davio
mesh_terms: D019659:Asteraceae / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects*; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology*; D009000:Monocytes / Q000502:physiology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D003374:Coumarins; D010936:Plant Extracts