title: Differential distribution of TASK-1, TASK-2 and TASK-3 immunoreactivities in the rat and human cerebellum.
abstract: In this work, the distributions of some acid-sensitive two-pore-domain K+ channels (TASK-1, TASK-2 and TASK-3) were investigated in the rat and human cerebellum. Astrocytes situated in rat cerebellar tissue sections were positive for TASK-2 channels. Purkinje cells were strongly stained and granule cells and astrocytes were moderately positive for TASK-3. Astrocytes isolated from the hippocampus, cerebellum and cochlear nucleus expressed TASK channels in a primary tissue culture. Our results suggest that TASK channel expression may be significant in the endoplasmic reticulum of the astrocytes. The human cerebellum showed weak TASK-2 immunolabelling. The pia mater, astrocytes, Purkinje and granule cells demonstrated strong TASK-1 and TASK-3 positivities. The TASK-3 labelling was stronger in general, but it was particularly intense in the Purkinje cells and pia mater.
authors.affiliation: Department of Physiology, Medical and Health Science Centre, University of Debrecen, 4012 Debrecen, Hungary. rz@phys.dote.hu
authors.forename: Z
authors.initials: Z
authors.lastname: Rusznák
authors.forename: K
authors.initials: K
authors.lastname: Pocsai
authors.forename: I
authors.initials: I
authors.lastname: Kovács
authors.forename: A
authors.initials: A
authors.lastname: Pór
authors.forename: B
authors.initials: B
authors.lastname: Pál
authors.forename: T
authors.initials: T
authors.lastname: Bíró
authors.forename: G
authors.initials: G
authors.lastname: Szücs
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D015153:Blotting, Western; D002478:Cells, Cultured; D002531:Cerebellum / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D010841:Pia Mater / Q000378:metabolism; D015221:Potassium Channels / Q000096:biosynthesis*; D024683:Potassium Channels, Tandem Pore Domain; D011689:Purkinje Cells / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: C410295:KCNK5 protein, human; C498655:KCNK9 protein, human; C498740:Kcnk5 protein, rat; C407082:Kcnk9 protein, rat; D009419:Nerve Tissue Proteins; D015221:Potassium Channels; D024683:Potassium Channels, Tandem Pore Domain; C420132:potassium channel subfamily K member 3