title: Reduction of acrylamide uptake by dietary proteins in a caco-2 gut model.
abstract: The report of elevated acrylamide levels in some foods raised an international health alarm, because acrylamide probably has carcinogenic, neurotoxic, and genotoxic properties. However, data on the bioavailability of acrylamide from food matrices in humans are limited. In particular, only little is known about the interactions of acrylamide with food ingredients. Using a human intestine model (Caco-2 cells), this study shows that acrylamide monomers are highly bioavailable and pass the cell monolayer via passive diffusion. Furthermore, acrylamide binds to dietary proteins such as chicken egg albumin under intestinal and cooking conditions. This binding reduces the concentration of acrylamide monomers and leads to a reduced uptake by Caco-2 cells. Hence, it is concluded that a protein-rich diet may reduce acrylamide uptake.
authors.affiliation: Institut für Pharmazie und Molekulare Biotechnologie, Universität Heidelberg, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Schabacker
authors.forename: Thomas
authors.initials: T
authors.lastname: Schwend
authors.forename: Michael
authors.initials: M
authors.lastname: Wink
mesh_terms: D020106:Acrylamide / Q000378:metabolism*; D001682:Biological Availability; D018938:Caco-2 Cells; D004044:Dietary Proteins / Q000378:metabolism / Q000494:pharmacology*; D004058:Diffusion; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D008954:Models, Biological
chemical_list: D004044:Dietary Proteins; D020106:Acrylamide