title: High-efficiency detection of severe acute respiratory syndrome virus genetic material.
abstract: A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log(10) times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log(10) times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.
authors.affiliation: Department of Virology, Institute of Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herder-Str. 11, 79104 Freiburg, Germany. weidmann@ukl.uni-freiburg.de
authors.forename: Manfred
authors.initials: M
authors.lastname: Weidmann
authors.forename: Paolo M D A
authors.initials: PM
authors.lastname: Zanotto
authors.forename: Friedemann
authors.initials: F
authors.lastname: Weber
authors.forename: Martin
authors.initials: M
authors.lastname: Spiegel
authors.forename: Hans Rheinhard
authors.initials: HR
authors.lastname: Brodt
authors.forename: Frank T
authors.initials: FT
authors.lastname: Hufert
mesh_terms: D001992:Bronchoalveolar Lavage Fluid / Q000821:virology; D006801:Humans; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics*; D012680:Sensitivity and Specificity
chemical_list: D012367:RNA, Viral