title: Subtilisin-catalyzed glycopeptide condensation.
abstract: This method describes the use of subtilisin-catalyzed peptide condensation to form a 15-residue glycopeptide from two smaller synthetic peptides. A 12-residue peptide ester is synthesized by solid-phase peptide synthesis using a PAM-modified Rink amide resin that allows the formation of a peptide ester suitable for subtilisin ligation. The 12-residue acyl donor peptide ester is then ligated to a 3-residue acyl acceptor glycopeptide amide using subtilisin (EC 3.4.21.62) in a buffered mixture of water and DMF (1:9).
authors.affiliation: Department of Chemistry, Indiana University, Bloomington, USA.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Tolbert
authors.forename: Chi-Huey
authors.initials: CH
authors.lastname: Wong
mesh_terms: D000577:Amides / Q000138:chemical synthesis; D000596:Amino Acids; D005449:Fluorenes; D006020:Glycopeptides / Q000138:chemical synthesis*; D012117:Resins, Synthetic; D020860:Subtilisin
chemical_list: D000577:Amides; D000596:Amino Acids; D005449:Fluorenes; D006020:Glycopeptides; D012117:Resins, Synthetic; C075825:Rink amide resin; D020860:Subtilisin