Photosystem II reconstitution into proteoliposomes: structure-function characterization.
This chapter discusses the photosystem II (PSII) reconstitution into proteoliposomes. In the first part of the chapter, protocols are outlined for the preparation of lipid bilayer vesicles (liposomes) constituted of individual thylakoid lipids or their mixtures for the preparation of PSII particles, and for the incorporation of the PSII particles into the liposomes. In the second part of the chapter, methodologies are described for the structure-function characterization of the PSII-lipid complexes (proteoliposomes). This includes the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) determination of the PSII proteins, measurement of oxygen-evolving activity of PSII in the proteoliposomes, and the study of structural changes of the PSII proteins upon their incorporation into the lipid bilayers by Fourier transform infrared (FTIR) spectroscopy.
Fragata
Mário
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Département de Chimie-Biologie, Université du Québec à Trois-Rivières, Québec, Canada.
D002736:Chloroplasts / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D001467:Hordeum / Q000737:chemistry; D007425:Intracellular Membranes / Q000737:chemistry; D008563:Membrane Lipids / Q000737:chemistry; D010100:Oxygen / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000737:chemistry* / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism; D011510:Proteolipids / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
D008563:Membrane Lipids; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D011510:Proteolipids; C020485:proteoliposomes; D010100:Oxygen