title: High phylogenetic diversity of transconjugants carrying plasmid pJP4 in an activated sludge-derived microbial community.
abstract: Transconjugants of plasmid pJP4, originating from an agar plate mating of a Pseudomonas putida donor with an activated sludge-derived microbial community, were isolated and identified by partial 16S rDNA sequencing. The transconjugant strains belonged to a variety of genera of the alpha-, beta-, gamabeta- and gamma-classes of the Proteobacteria, mostly to the families Rhizobiaceae and Comamonadaceae and the genus Stenotrophomonas. Only P. putida and Delftia spp. strains were able to grow on 2,4-D as the sole carbon source.
authors.affiliation: Institute of Water Quality Control and Waste Management, Technical University of Munich, Am Coulombwall, 85748 Garching, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Bathe
authors.forename: Michael
authors.initials: M
authors.lastname: Lebuhn
authors.forename: Joachim W
authors.initials: JW
authors.lastname: Ellwart
authors.forename: Stefan
authors.initials: S
authors.lastname: Wuertz
authors.forename: Martina
authors.initials: M
authors.lastname: Hausner
mesh_terms: D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D017753:Ecosystem; D014644:Genetic Variation; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics*; D020560:Proteobacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016958:Pseudomonas putida / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012722:Sewage