Synthesis and characterization of deoxyribonucleic acid-conjugated gold nanoparticles.
Gold nanoparticles functionalized with thiol-modified single-stranded oligonucleotides are highly useful reagents for a variety of applications, ranging from materials science to bioanalytics. In this chapter, the preparation of citrate stabilized 15-nm Au nanoparticles is described. The nanoparticles are conjugated with 3'-thiol-modified deoxyribonucleic acid oligomers and the resulting conjugates are characterized by determining their shape, size, and surface coverage. The hybridization capabilities are quantified in a microplate assay.
Hazarika
Pompi
P
Biologisch-Chemische Mikrostrukturtechnik, Fachbereich Chemie der Universität Dortmund, Germany.
Giorgi
Tatiana
T
Reibner
Martina
M
Ceyhan
Buelent
B
Niemeyer
Christof M
CM
D004247:DNA / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D017979:Gold Colloid / Q000138:chemical synthesis; D008854:Microscopy, Electron; D036103:Nanotechnology / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016622:Silver Staining; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D019809:Streptavidin
D017979:Gold Colloid; D009838:Oligodeoxyribonucleotides; D006046:Gold; D004247:DNA; D019809:Streptavidin