title: [Establishment and characterization of human alloantigen induced T cell clones].
abstract: OBJECTIVE
  To estabilish and analyze stable human T cell clones cultured in-vitro.
  
  
  METHODS
  The activated T cells from mixed lymphocyte culture (MLC) were cloned by limiting dilution using irradiated PBMCs as feeder cells. T cell clones were then characterized by immunofluorescence staining and flow cytometry analysis.
  
  
  RESULTS
  16 clones were obtained, of which all but one were alphabetaT cells. Among the 15 alphabetaT cell clones, 9 clones belonged to Th subset, including 3 Th0 and 6 Th2, and 6 clones belonged to Tc subset, including 5 Tc0 and 1 Tc2.
  
  
  CONCLUSIONS
  Fifteen human T cell clones were successfully established, which lays the foundation for study on markers and function of Tc1 and Tc2 subsets.
authors.affiliation: Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Wei-ning
authors.initials: WN
authors.lastname: Han
authors.forename: Yun-xin
authors.initials: YX
authors.lastname: Cao
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Xie
authors.forename: Xue-song
authors.initials: XS
authors.lastname: Liu
authors.forename: Bo-quan
authors.initials: BQ
authors.lastname: Jin
mesh_terms: D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D015789:HLA-A2 Antigen / Q000032:analysis; D006801:Humans; D007519:Isoantigens / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D000098862:T Lineage-Specific Activation Antigen 1
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D000098862:T Lineage-Specific Activation Antigen 1; D015789:HLA-A2 Antigen; D007519:Isoantigens