Sequence-specific Rho-RNA interactions in transcription termination.
The bacteriophage lambda tR1 terminator encodes a region of the nascent cro transcript containing RNA residues recognized by termination factor Rho. To identify ribonucleotide-protein interactions contributing to termination, a library of reporter gene plasmids was constructed containing predominantly single-nucleotide substitutions in a 24 nt region previously shown to be critical for efficient termination. Screening 16 822 bacterial transformants identified 110 terminator mutants, most of which contained two or more nucleotide substitutions. Although the vast majority of single base changes did not reduce tR1 function, 11 specific single-nucleotide substitutions at eight positions interspersed in the upstream part of the target region (5'-ATAACCCCGCTCTT ACACATTCCA-3') did reduce termination. About half of these substitutions also reduced Rho-dependent termination on cro gene templates transcribed by purified RNA polymerase, indicating specific residues critical for optimal terminator function. Other termination defects were not reproduced in these in vitro assays, and likely resulted from indirect effects of altering interactions between tR1 and additional cellular factors capable of attenuating Rho function. Our results indicate that while Rho is able to recognize a wide variety of similar rut site sequences by interacting with alternate nucleotides at critical positions, interactions with specific individual ribonucleotides of the tR1 transcript provide highly efficient Rho-dependent termination.
Graham
James E
JE
Department of Biology, Indiana University, Bloomington, IN 47405, USA. j.graham@louisville.edu
D000251:Adenosine Triphosphatases / Q000378:metabolism; D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D004268:DNA-Binding Proteins; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral / Q000235:genetics; D016296:Mutagenesis / Q000235:genetics; D009154:Mutation / Q000235:genetics; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics; D012234:Rho Factor / Q000378:metabolism*; D013379:Substrate Specificity; D013728:Terminator Regions, Genetic / Q000235:genetics*; D013816:Thermodynamics; D014158:Transcription, Genetic / Q000235:genetics*; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins
D004268:DNA-Binding Proteins; D012367:RNA, Viral; D012097:Repressor Proteins; D012234:Rho Factor; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins; D000251:Adenosine Triphosphatases