[Blood levels of the adhesion molecules ICAM-1 and VCAM-1 in patients with chronic myeloid leukemia during remission and exacerbation].
A change in ICAM-1 and VCAM-1 concentrations is most likely to occur during the course of chronic myleoid leukemia (CML). The purpose of our study was to examine concentrations of these adhesion molecules in blood plasma, culture supernatant and isolated, broken granulocytes in 20 patients (45-65 years old) with CML in exacerbation and during the remission of the disease and in 10 healthy control subjects. The concentration assay of substances mentioned above was made using ready immunoenzymatic sets of ELISA type. The examinations were carried out on the cultures of cells stimulated and nonstimulated with mitogen--Neupogen of Roche with a the dose of 1 mu/4 ml of culture. Mitogen was added to activate granulocytes and to induce blastic transformation. A significant increase in of plasma ICAM-1 concentration was found in CML exacerbation and remission. The difference between the concentrations of the adhesion molecules with mitogen stimulated and nonstimulated cultures were observed. A significant increase in ICAM-1 and VCAM-1 concentration could suggest a higher secretory function of granulocytes. The values of ICAM-1 were increased in culture supernatants and broken granulocytes before and after adding mitogen in comparison to control groups. The difference in concentration we observed could be characteristic for leukaemic cells.
Kiersnowska-Rogowska
Beata
B
Klinika Hematologii Akademii Medycznej w Białymstoku.
Parfieńczyk
Adam
A
Rogowski
Franciszek
F
D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000097:blood*; D012074:Remission Induction; D019010:Vascular Cell Adhesion Molecule-1 / Q000097:blood*
D014408:Biomarkers, Tumor; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1