The effect of naringin, a bioflavonoid on ischemia-reperfusion induced renal injury in rats.
There is increasing evidence to suggest that toxic oxygen radicals play a role in the pathogenesis of ischemia/reperfusion (I/R) injury in the kidney. This study was designed to investigate the effects of naringin (Ng), a bioflavonoid in I/R induced renal failure in rats. The protective effect of naringin against the damage inflicted by reactive oxygen species (ROS) during renal I/R was investigated in Sprague-Dawley rats using histopathological and biochemical parameters. In one set of experiments animals were unilaterally nephrectomized, and subjected to 45 min of left renal pedicle occlusion and in another set both the renal pedicles were occluded for 45 min followed by 24h of reperfusion. Naringin (400 mg kg(-1), p.o.) was administered 60 min prior to ischemia. At the end of the reperfusion period, rats were sacrificed. Thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH) levels, catalase (CAT), and superoxide dismutase (SOD) activities were determined in renal tissue. Serum creatinine and blood urea nitrogen (BUN) concentrations were measured for the evaluation of renal function. Ischemic control animals demonstrated severe deterioration of renal function, renal morphology and a significant renal oxidative stress. Pretreatment of animals with naringin markedly attenuated renal dysfunction, morphological alterations, reduced elevated TBARS levels and restored the depleted renal antioxidant enzymes. The findings imply that ROS play a causal role in I/R induced renal injury and naringin exert renoprotective effects probably by the radical scavenging and antioxidant activities.
Singh
Devinder
D
Pharmacology Division, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh 160014, India. dr_chopra_k@yahoo.com
Chopra
Kanwaljit
K
D000284:Administration, Oral; D000818:Animals; D001806:Blood Urea Nitrogen; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D003404:Creatinine / Q000378:metabolism; D004195:Disease Models, Animal; D044950:Flavanones / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*; D005419:Flavonoids / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*; D005609:Free Radicals / Q000378:metabolism; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism; D007668:Kidney / Q000098:blood supply* / Q000293:injuries / Q000648:ultrastructure; D007674:Kidney Diseases / Q000188:drug therapy* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009392:Nephrectomy; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D044950:Flavanones; D005419:Flavonoids; D005609:Free Radicals; D017392:Thiobarbituric Acid Reactive Substances; D003404:Creatinine; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; C005274:naringin