Application of NMR and EPR methods to the study of RNA.
The application of techniques based on magnetic resonance, specifically electron paramagnetic resonance (EPR) and nuclear magnetic resonance (NMR), has provided a wealth of new information on RNA structures, as well as insights into the dynamics and function of these important biomolecules. NMR spectroscopy is very successful for determining the solution structures of small RNA domains, aptamers and ribozymes, and exploring their intramolecular dynamics and interactions with ligands. EPR-based methods have been used to map local dynamic and structural features of RNA, to explore different modes of RNA-ligand interaction, to obtain long-range structural restraints and to probe metal-ion-binding sites.
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Peter Z
PZ
Department of Chemistry, University of Southern California, LJS-251, 840 Downey Way, Los Angeles, California 90089-0744, USA. pzq@usc.edu
Dieckmann
Thorsten
T
D001665:Binding Sites; D015195:Drug Design; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008670:Metals / Q000378:metabolism; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D013113:Spin Labels
D008024:Ligands; D008670:Metals; D013113:Spin Labels; D012313:RNA