Molecular microbial ecology: land of the one-eyed king.
Studies of microbial biodiversity have made astounding discoveries of late due to the use of methodologies based on phylogenetic analyses of small subunit ribosomal RNA sequences. Although there are limitations to these methods, they can nonetheless be very useful if these limitations are kept in mind. These limitations range from technical problems such as obtaining representative genomic DNA and suitable primers, to conceptual problems such as defining and using meaningful taxonomic units of diversity (species). Here we discuss several of the limitations inherent in studies of microbial diversity that must be considered when interpreting the results obtained using these approaches.
Forney
Larry J
LJ
Department of Biological Sciences, University of Idaho, Moscow, Idaho, USA. lforney@uidaho.edu
Zhou
Xia
X
Brown
Celeste J
CJ
D001105:Archaea / Q000145:classification* / Q000235:genetics; D001419:Bacteria / Q000145:classification* / Q000235:genetics; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000032:analysis; D017753:Ecosystem; D004586:Electrophoresis; D007778:Lactobacillus / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D016415:Sequence Alignment
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S