Liquid-liquid extraction of xylitol dehydrogenase from Candida guilliermondii homogenate by reversed micelles.
The intracellular enzyme xylitol dehydrogenase (XD, EC 1.1.1.9) from Candida guilliermondii, grown in sugarcane bagasse hydrolysate, was separated by reversed micelles of BDBAC [N-benzyl-N-dodecyl-N-bis (2-hydroxyethyl) ammonium chloride] cationic surfactant. An experimental design was employed to evaluate the influence of the following factors on the enzyme separation: temperature, co-solvent concentration and surfactant concentration. The results showed that just the temperature did not show significant effect on XD recovery. A model was used to represent the activity recovery and fit the experimental data. Under optimized conditions, the recovery of total activity was about 121%, and the purity increased 2.3-fold.
Cortez
Ely Vieira
EV
Biochemical and Pharmaceutical Technology Department/FCF, University of São Paulo, P O Box 66083, CEP 05315-970 São Paulo, SP, Brazil.
Pessoa
Adalberto
A
Felipe Md
Maria das Graças de Almeida
Md
Roberto
Inês Conceição
IC
Vitolo
Michele
M
D002175:Candida / Q000201:enzymology*; D002474:Cell-Free System; D050542:D-Xylulose Reductase; D008823:Micelles; D013401:Sugar Alcohol Dehydrogenases / Q000302:isolation & purification*; D013501:Surface-Active Agents
D008823:Micelles; D013501:Surface-Active Agents; D013401:Sugar Alcohol Dehydrogenases; D050542:D-Xylulose Reductase