Separation, purification, and characterization of polypeptide composition of subcomplexes of the main light-harvesting chlorophyll a/b-protein complex of photosystem II.
The protocols regarding separation and purification of LHCII subcomplexes by non-denaturing isoelectric focusing (ndIEF), confirmation of trimeric nature of the subcomplexes, and establishing their polypeptide composition are described below.
Jackowski
Grzegorz
G
Adam Mickiewicz University, Department of Plant Physiology, Poznan, Poland.
D017360:Arabidopsis / Q000737:chemistry; D002942:Circular Dichroism / Q000379:methods; D029749:Dianthus / Q000737:chemistry; D007425:Intracellular Membranes / Q000737:chemistry; D007525:Isoelectric Focusing / Q000379:methods; D045342:Light-Harvesting Protein Complexes / Q000235:genetics / Q000302:isolation & purification*; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification*; D045332:Photosystem II Protein Complex / Q000235:genetics / Q000302:isolation & purification*; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification*; D011487:Protein Conformation; D021122:Protein Subunits / Q000235:genetics / Q000302:isolation & purification*; D020524:Thylakoids / Q000737:chemistry
D045342:Light-Harvesting Protein Complexes; D010455:Peptides; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D021122:Protein Subunits