Characterization of the bombesin-like peptide receptor family in primates.
In mammals, bombesin-like peptides mediate a broad range of physiological functions through binding to three highly conserved G-protein-coupled receptors: the neuromedin B-preferring, the gastrin-releasing peptide-preferring, and the bombesin-receptor subtype 3. Selective modulation of these receptors presents opportunities for the development of novel therapeutics. To ascertain if rhesus monkey could serve as a surrogate animal model for the development of modulators of bombesin-like receptor function, we undertook a search for additional receptor family members and studied the expression profiles of the three known bombesin-related receptors. We found no evidence for additional receptor family members in mammals, suggesting that the expression of the previously described bombesin-receptor subtype 4 is limited to amphibians. We studied the distribution of the three receptors in a broad array of human and rhesus monkey tissues. Based on the similarity between the human and the rhesus expression profiles, we conclude that the rhesus monkey may be a suitable animal model to evaluate the clinical efficacy and potential side effects of bombesin-like peptide ligands.
Sano
Hideki
H
Department of Metabolic Disorders, Merck Research Laboratories, P.O. Box 2000, Rahway, NJ 07065, USA.
Feighner
Scott D
SD
Hreniuk
Donna L
DL
Iwaasa
Hisashi
H
Sailer
Andreas W
AW
Pan
Jie
J
Reitman
Marc L
ML
Kanatani
Akio
A
Howard
Andrew D
AD
Tan
Carina P
CP
D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000235:genetics / Q000378:metabolism; D000663:Amphibians / Q000235:genetics / Q000378:metabolism; D000818:Animals; D019886:Gastrin-Releasing Peptide / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology; D006801:Humans; D008253:Macaca mulatta / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D015287:Neurokinin B / Q000031:analogs & derivatives* / Q000378:metabolism; D009928:Organ Specificity / Q000235:genetics; D018004:Receptors, Bombesin / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity
D029845:Amphibian Proteins; D018004:Receptors, Bombesin; D019886:Gastrin-Releasing Peptide; D015287:Neurokinin B; C038393:neuromedin B