Quantification of solid cell material by detection of membrane-associated proteins and peptidoglycan.
Quantification of solid cell material (cell debris) is necessary for the optimisation of the efficiency of bioseparations. Cell debris can be quantified by detection of a component present in the cell wall that can act as a marker for cell debris. Membrane-associated proteins have previously been used as a marker for cell debris. This marker was quantified by SDS-PAGE with densiometry. In this paper cell debris quantification methods are presented that are faster and more accurate, i.e. membrane-associated protein quantification with the Protein 50 Labchip of Agilent Technologies, or that make use of peptidoglycan as marker for cell debris, i.e. a spectrophotometric muramic acid assay.
van Hee
P
P
Department of Biotechnology, Delft University of Technology Julianalaan 67, 2628 BC Delft, The Netherlands.
Middelberg
A P J
AP
van der Lans
R G J M
RG
van der Wielen
L A M
LA
D002849:Chromatography, Gas; D004591:Electrophoresis, Polyacrylamide Gel; D005285:Fermentation; D008565:Membrane Proteins / Q000032:analysis*; D010457:Peptidoglycan / Q000032:analysis*
D008565:Membrane Proteins; D010457:Peptidoglycan