title: Noggin gene delivery inhibits cementoblast-induced mineralization.
abstract: Bone morphogenetic proteins (BMPs) are known to promote periodontal tissue regeneration, while noggin inhibits the biological activities of BMP-2, -4, and -7. To investigate the effect of BMPs and noggin gene transfer on cementogenesis, we used cloned murine cementoblasts (OCCM). Cells were transduced using adenoviruses encoding BMP-7 (Ad-BMP-7), noggin devoid of the heparin binding site (Ad-NOGDeltaB2), or a control adenovirus encoding green fluorescent protein (Ad-GFP). Cells were seeded into 3D polymer scaffolds and implanted into SCID mice to determine the in vivo mineral-inducing ability of the cells. Cells transduced with Ad-NOGDeltaB2 at 3 and 6 weeks postimplantation exhibited reduced mineral formation compared with all other groups. Although gene expression of osteocalcin and bone sialoprotein increased after Ad-BMP-7 transduction in vitro, following BMP-7 gene transfer in vivo, transcripts for OCN and BSP were not significantly different from controls, and mineral density was not significantly increased compared with Ad-GFP and NT groups. These results indicate that in mature cementoblast populations, gene transfer of noggin inhibits biomineralization induced by cementoblasts, whereas exogenous BMP has minimal effects on mineralization.
authors.affiliation: Center for Craniofacial Regeneration and Department of Periodontics/Prevention/Geriatrics, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109-1078, USA.
authors.forename: Q-M
authors.initials: QM
authors.lastname: Jin
authors.forename: M
authors.initials: M
authors.lastname: Zhao
authors.forename: A N
authors.initials: AN
authors.lastname: Economides
authors.forename: M J
authors.initials: MJ
authors.lastname: Somerman
authors.forename: W V
authors.initials: WV
authors.lastname: Giannobile
mesh_terms: D000818:Animals; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000494:pharmacology; D002352:Carrier Proteins; D002454:Cell Differentiation / Q000187:drug effects; D002461:Cell Line, Transformed; D025021:Cementogenesis / Q000187:drug effects*; D002999:Clone Cells; D003739:Dental Cementum / Q000166:cytology / Q000502:physiology*; D018014:Gene Transfer Techniques; D051379:Mice; D016513:Mice, SCID; D008903:Minerals / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000494:pharmacology*; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000494:pharmacology; D000099176:Noggin Protein
chemical_list: D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D002352:Carrier Proteins; D008903:Minerals; D011506:Proteins; D016212:Transforming Growth Factor beta; D000099176:Noggin Protein