[Establishment of methods of mechanical dissociation and culture of spiral ganglion neurons in postnatal mice].
OBJECTIVE
To conduct a study on the methods for establishing a cytological model of mechanical dissociation and culture of spiral ganglion neurons (SGNs) in postnatal mice.


METHODS
The spiral ganglion neurons were taken from 1-6-day old mice for primary culture. The morphology and the process of axon were observed by use of an inverted/phase contrast microscope. Immunocytochemical identification was performed using fluorescent microscopy.


RESULTS
In the state of primary culture in vitro, the SGNs were found to have good cell morphology and axon regeneration power, the surviving cells of SGN(5-7 d) amounted to (78.10 +/- 4.33)/cm2 and the survival period was 7-14 d.


CONCLUSIONS
We successfully established the methods of mechanical dissociation and culture of SGN cytological model; the cell amount and survival period of SGNs were able to meet the requirement of cytological experiments.
Deng
Jia-hong
JH
Department of Otolaryngology, West China Hospital of Sichuan University, Chengdu 610041, China.
Fan
Jian-gang
JG
Liang
Chuan-yu
CY
Tang
Yue-di
YD
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D003051:Cochlea / Q000166:cytology; D051379:Mice; D009474:Neurons / Q000166:cytology*; D013136:Spiral Ganglion / Q000166:cytology*