[Study on extraction and isolation of active constituents from Sorbaria sorbifolia and antitumor effect of the constituents in vivo].
OBJECTIVE
To study the constituents from Sorbaria sorbifolia and their antitumor activities.


METHODS
The constituents were isolated with silia gel column chromatography and identified by physicochemical properties and spectroscopic analysis. Thirty six mice were inoculated with sarcocarcinoma 180 according to the standard method, Sorbaria sorbifolia ethyl acetate etract was administrated for 10 days, tumor and body weight, the activities of NK cell and the contents of TNF-alpha, IL-2 in serum were detected.


RESULTS
Three compounds were isolated from the ethyl acetate extract and identified as 5,2',4'-trihydroxy-6,7,5'-trimethoxyflavone(I), succinic acid(II) and p-hydroxybenzoic acid(III). The tumor of the test groups were lighter than that of control group(P < 0.05). There was no remarkable difference between the control and test groups in body weight (P > 0.05). The activity of NK cell and the contents of TNF-alpha, IL-2 in serum were higher than that of control group (P < 0.05).


CONCLUSIONS
Compounds I and II were isolated from Sorbaria sorbifolia ethyl acetate extract for the first time. The ethyl acetate extract could improve immune function and strengthen antitumor effect in sarcocarcinoma 180.
Zhang
Xuewu
X
Medical College, Yanbian University, Yanji, Jilin 133000.
Zhang
Yumei
Y
Guan
Liping
L
Quan
Yingchun
Y
Sun
Quan
Q
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D047309:Flavones / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology; D007376:Interleukin-2 / Q000097:blood; D051379:Mice; D010226:Parabens / Q000032:analysis / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry; D027824:Rosaceae / Q000737:chemistry*; D012510:Sarcoma 180 / Q000473:pathology*; D019802:Succinic Acid / Q000032:analysis / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
C494889:5,2',4'-trihydroxy-6,7,5'-trimethoxyflavone; D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D047309:Flavones; D007376:Interleukin-2; D010226:Parabens; D014409:Tumor Necrosis Factor-alpha; D019802:Succinic Acid; C038193:4-hydroxybenzoic acid