Identification of key immune mediators regulating T helper 1 responses in swine.
This publication describes the cloning of full or partial length sequences for pig TBX21 (T-bet), MYD88, ICSBP1, CD8A (CD8alpha), CD8B (CD8beta), and CD28 cDNAs. Real-time PCR assays have been developed for the relative quantitation of these products as well as previously characterized transcripts that encode exon A-containing CD45, HLX1, IRF1, STAT1 and RPL32. When used for examining temporal immune gene expression in the liver of Toxoplasma gondii infected pigs, the positive regulators of Th1 responses, IRF1, MYD88, and STAT1, were found to be expressed prior to the simultaneous upregulation of interferon gamma (IFNG), HLX1 and TBX21 gene expression. In contrast, in the mesenteric lymph node (MLN), only expression of IRF1 and IFNG was significantly upregulated. Based on their demonstrated utility in establishing an immune response pathway, these PCR assays should be valuable additions to our swine immune toolkit.
Dawson
Harry D
HD
Nutrient Requirements and Functions Laboratory, Beltsville Human Nutrition Research Center, USDA, Bldg. 307C, Room 213, BARC-East, Beltsville, MD 20705, USA.
Royaee
Atabak R
AR
Nishi
Sandra
S
Kuhar
Daniel
D
Schnitzlein
William M
WM
Zuckermann
Federico
F
Urban
Joseph
J
Lunney
Joan K
JK
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D018106:CD28 Antigens / Q000235:genetics / Q000276:immunology; D016827:CD8 Antigens / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D053594:Myeloid Differentiation Factor 88; D016053:RNA, Protozoan / Q000737:chemistry / Q000235:genetics; D011971:Receptors, Immunologic / Q000235:genetics / Q000276:immunology; D012097:Repressor Proteins / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000469:parasitology*; D020825:T-Box Domain Proteins / Q000235:genetics / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*; D014122:Toxoplasma / Q000235:genetics / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000276:immunology* / Q000469:parasitology
D048868:Adaptor Proteins, Signal Transducing; D000943:Antigens, Differentiation; D018106:CD28 Antigens; D016827:CD8 Antigens; C431698:CD8alpha antigen; C477867:CD8beta antigen; D053594:Myeloid Differentiation Factor 88; D016053:RNA, Protozoan; D011971:Receptors, Immunologic; D012097:Repressor Proteins; D020825:T-Box Domain Proteins