Laser spectral characterization in multiphoton microscopy.
Spectral and temporal characterization is a fundamental task when a tunable Ti:sapphire ultrafast laser system is operated for multiphoton microscopy applications. In the present paper simple procedures are reported that perform laser-peak-emission wavelength and bandwidth measurements without the need of any further instrumentation but a simple and inexpensive diffraction grating, by taking advantage of the confocal microscope imaging capabilities.
Quercioli
Franco
F
Istituto Nazionale di Ottica Applicata, Laboratorio di Biofotonica, Largo Enrico Fermi 6, 50125 Firenze, Italy. quercioli@ino.it
Tiribilli
Bruno
B
Vassalli
Massimo
M
Ghirelli
Alessandro
A
D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods*; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods*; D012031:Refractometry / Q000295:instrumentation* / Q000706:statistics & numerical data*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity