title: Ruling out Bacillus anthracis.
abstract: Optimization of methods for ruling out Bacillus anthracis leads to increased yields, faster turnaround times, and a lighter workload. We used 72 environmental non-B. anthracis bacilli to validate methods for ruling out B. anthracis. Most effective were the use of horse blood agar, motility testing after isolates had a 2-h incubation in trypticase soy broth, and screening isolates with a B. anthracis-selective agar.
authors.affiliation: National and Kapodistrian University of Athens Medical School, Athens, Greece.
authors.forename: Joseph
authors.initials: J
authors.lastname: Papaparaskevas
authors.forename: Dimitra P
authors.initials: DP
authors.lastname: Houhoula
authors.forename: Maria
authors.initials: M
authors.lastname: Papadimitriou
authors.forename: Georgios
authors.initials: G
authors.lastname: Saroglou
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Legakis
authors.forename: Loukia
authors.initials: L
authors.lastname: Zerva
mesh_terms: D000818:Animals; D001408:Bacillus anthracis / Q000302:isolation & purification*; D023482:Bioterrorism; D003470:Culture Media; D006461:Hemolysis; D006801:Humans; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies
chemical_list: D003470:Culture Media