Searching for novel CYP members using cDNA library from a minke whale liver.
The contaminant-induced cytochrome P450 (CYP) members in minke whale (Balaenoptera acutorostrata) can be potential biomarkers of the contaminant exposure and toxic effects. In this study, we constructed a cDNA library from the liver of minke whale from the North Pacific, and further screened a total of 6930 clones randomly selected in the library for the isolation of cDNA clones encoding novel members of CYP superfamily. The screening revealed the isolation of six novel CYP cDNA clones that are classified into CYP1A, CYP2C, CYP2E, CYP3A, CYP4, and CYP4A subfamilies. The BLAST homology search using the partial cDNA fragments of four CYP subfamilies (CYP1A, CYP2C, CYP2E and CYP4A) demonstrated that the minke whale CYPs were most closely related to pig CYPs (81-91%). Identification of multiple CYP genes in marine mammal species such as minke whale will provide new insights into the metabolic or toxicological functions of individual CYP members.
Kim
Eun-Young
EY
Ehime Prefectural Institute of Public Health and Environmental Sciences, 8-234 Sankan-cho, Matsuyama 990-0003, Japan.
Iwata
Hisato
H
Fujise
Yoshihiro
Y
Tanabe
Shinsuke
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015415:Biomarkers; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D030541:Databases, Genetic; D015723:Gene Library; D008099:Liver / Q000737:chemistry*; D008297:Male; D008969:Molecular Sequence Data; D010140:Pacific Ocean; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014907:Whales / Q000235:genetics*
D015415:Biomarkers; D003577:Cytochrome P-450 Enzyme System