Comparison of versions 1.0 AND 1.5 of the UltraSensitive AMPLICOR HIV-1 MONITOR test for subjects with low viral load.
We compared the performance of two UltraSensitive AMPLICOR HIV-1 MONITOR kits (version 1.5 [v1.5] versus v1.0) by retesting 404 plasma samples with low viral loads (<3,000 copies/ml) with both kits. With 292 samples that initially had <50 copies/ml by the v1.0 kit, the v1.5 assay was more sensitive than the v1.0 assay for samples with human immunodeficiency virus type 1 RNA near the 50-copy/ml cutoff (P = 0.0146). Median numbers of copies per milliliter were similar for 112 samples with 50 to 3,000 copies/ml with no difference in sensitivity with a 200-copy/ml cutoff.
Jackson
J Brooks
JB
Johns Hopkins Medical Institutions, Carnegie Building, Room 415, 600 North Wolfe St., Baltimore, MD 21287, USA. bjackso@jhmi.edu
Piwowar-Manning
Estelle
E
Johnson-Lewis
LeTanya
L
Bassett
Roland
R
Demeter
Lisa M
LM
Brambilla
Donald
D
D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D012367:RNA, Viral / Q000032:analysis*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D019562:Viral Load
D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic