Applications of single-molecule detection to the analysis of pathogenic DNA.
We have devised a new technique based on single fluorescent molecule detection for the analysis of specific sequences of DNA. The method consists of synthesizing a fluorescent reporter molecule using a polymerase extension reaction and labeled nucleotides. The fluorescent reporter products are analyzed in a laser-based single-molecule detection system. We have applied this method to the detection of pUC19 and Bacillus anthracis DNA targets. We expect that this method will have applications in rapid detection and identification of DNA from pathogens as well as other sources, and that it will be used for processing of large number of samples in a short period of time.
Marina
Oana
O
Physics Division, Los Alamos National Laboratory, Los Alamos, NM, USA.
Castro
Alonso
A
D001408:Bacillus anthracis / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D004784:Environmental Monitoring / Q000379:methods; D017930:Genes, Reporter / Q000235:genetics; D008967:Molecular Biology / Q000379:methods; D008969:Molecular Sequence Data; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D004269:DNA, Bacterial