title: Decreased histamine-stimulated phosphoinositide hydrolysis in the cerebral cortex of a rat line selectively bred for high alcohol preference.
abstract: This study sheds light on the comparative analysis of agonist-stimulated phosphoinositide (PI) hydrolysis in the cerebral cortex of alcohol-naive rats from established lines selectively bred for low alcohol preference (LAP) and high alcohol preference (HAP). The effect of histamine (1.0 mM), but neither norepinephrine (0.1 mM) nor carbachol (0.5 mM), on PI hydrolysis was significantly reduced in HAP rats (0.4 +/- 5.0 fmol/mg protein [3H]inositol phosphates formed over basal) compared with LAP rats (25.5 +/- 10.0 fmol/mg protein). The contents of monoamines (dopamine, norepinephrine, and serotonin) and histamine in the cerebral cortex did not significantly differ between LAP and HAP rats, nor did the contents of their metabolites, except 3-methoxy-4-hydroxyphenylglycol (one of the metabolites of norepinephrine) and N(tau)-methylhistamine, which was not detected in our system. The histamine stimulatory effect was unchanged in the cerebral cortex of an intact Wistar rat that was treated with intraperitoneal injection of alcohol (1.0 g/kg once per day for 14 days). The results of the current study indicate that the decrease in the histamine effect on PI hydrolysis in HAP rats might be attributed to that particular rat line.
authors.affiliation: Department of Pharmacology, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan.
authors.forename: Nobue
authors.initials: N
authors.lastname: Kitanaka
authors.forename: Junichi
authors.initials: J
authors.lastname: Kitanaka
authors.forename: Minori
authors.initials: M
authors.lastname: Nishiguchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kinoshita
authors.forename: Harumi
authors.initials: H
authors.lastname: Ouchi
authors.forename: Takako
authors.initials: T
authors.lastname: Minami
authors.forename: Shigeru
authors.initials: S
authors.lastname: Hishida
authors.forename: Motohiko
authors.initials: M
authors.lastname: Takemura
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D004361:Drug Tolerance / Q000235:genetics; D000431:Ethanol / Q000494:pharmacology*; D005518:Food Preferences / Q000187:drug effects / Q000502:physiology; D006632:Histamine / Q000494:pharmacology*; D006868:Hydrolysis; D010716:Phosphatidylinositols / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D010716:Phosphatidylinositols; D000431:Ethanol; D006632:Histamine