title: Brachyspira pilosicoli isolated from two beagles and one mongrel in Japan.
abstract: The feces, colonic contents, and colonic mucosa from two beagles and a mongrel with and without mucohemorrhagic diarrhea were anaerobically cultured and after 72 hr, lucent and rough colonies with weak beta-hemolysis were observed. Small spirochetes with one or two loose waves in the colonies were observed under a phase contrast microscope and the spirochete cells sometimes aggregated. The 16SrDNA sequencing results demonstrated that the canine isolates were B. pilosicoli and the base alignments registered in DDBJ. This is the first report concerning the isolation of B. pilosicoli from dogs in Japan.
authors.affiliation: Animal Health Laboratory, School of Agriculture, Ibaraki University, Ami, Japan.
authors.forename: Masami
authors.initials: M
authors.lastname: Manabe
authors.forename: Itaru
authors.initials: I
authors.lastname: Suenaga
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Ogawa
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Adachi
mesh_terms: D000818:Animals; D001483:Base Sequence; D003106:Colon / Q000382:microbiology; D004275:DNA, Ribosomal / Q000235:genetics; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D005243:Feces / Q000382:microbiology; D007564:Japan; D008858:Microscopy, Phase-Contrast; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013144:Spirochaetales / Q000235:genetics*; D013145:Spirochaetales Infections / Q000662:veterinary*
chemical_list: D004275:DNA, Ribosomal