Isolation and characterisation of an Indian strain of Mycoplasma mycoides subsp. mycoides type LC from a case of caprine arthritis.
Isolation and characterisation of an Indian strain of Mycoplasma mycoides subsp. mycoides LC from a case of caprine arthritis is reported in the study. The isolate was identified based on biochemical, digitonin sensitivity and growth inhibition tests. The virulence of the organism was studied by pathogenicity test in mice and goat. The antigenic and genomic profile of the isolate was compared with that of the standard strain (Y-Goat). Using different sets of primers, polymerase chain reaction was employed for rapid detection of the strain.
Singh
Vijendra P
VP
National Referral Laboratory on Mycoplasma, Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, UP, India. vijendra@ivri.up.nic.in
Srivastava
N C
NC
Kumar
Manoj
M
Sunder
M Jai
MJ
Varshney
J P
JP
D000818:Animals; D001168:Arthritis / Q000150:complications / Q000276:immunology / Q000662:veterinary*; D001426:Bacterial Proteins / Q000378:metabolism; D015153:Blotting, Western / Q000662:veterinary; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D015511:Goat Diseases / Q000276:immunology / Q000382:microbiology*; D006041:Goats; D007194:India; D051379:Mice; D009176:Mycoplasma mycoides / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000472:pathogenicity; D011002:Pleuropneumonia, Contagious / Q000276:immunology / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D014774:Virulence
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes