In situ-formed, tissue-adhesive co-gel composed of styrenated gelatin and styrenated antibody: potential use for local anti-cytokine antibody therapy on surgically resected tissues.
Styrenated antibody (ST-Ab) and styrenated gelatin (ST-gelatin) were prepared by condensation reaction of antibody or gelatin with 4-vinylbenzoic acid, respectively. The affinity loss of ST-Ab to its antigen was minimal. ST-Ab and ST-gelatin were copolymerized with by visible-light irradiation in the presence of a water-soluble camphorquinone as a photoinitiator to produce a tissue-adhesive, in situ-formed co-gel of ST-gelatin and ST-Ab. The amount of non-reacted ST-Ab released from the co-gel of ST-gelatin and ST-Ab into the medium was minimal. The confocal laser scanning microscopy observation showed that local accumulation of rhodamine-labeled bovine serum albumin (BSA) as a model antigen was noticed in the surface-to-subsurface region of the co-gel of ST-gelatin and anti-BSA ST-Ab, indicating that the gel prevented the permeation of BSA into the gel. In invasion double chamber assay using anti-hepatocyte growth factor (HGF) antibody, the co-gel prevented HGF-dependent invasion of pancreatic cancer cells. The discussion was made for potential application of an in situ-formed tissue-adhesive co-gel of ST-gelatin and ST-Ab, developed in this study, as a cytokine-barrier on a surgically resected tissue where cancer cells might still remain after resection of cancerous tissue.
Manabe
Tatsuya
T
Division of Surgery and Oncology, Graduate School of Medicine, Kyushu University, 3-1-1 Maidashi, Fukuoka 812-8582, Japan.
Okino
Hidenobu
H
Tanaka
Masao
M
Matsuda
Takehisa
T
D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D000941:Antigens / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D003094:Collagen / Q000737:chemistry; D004396:Coloring Agents / Q000494:pharmacology; D003470:Culture Media; D016207:Cytokines / Q000737:chemistry; D004338:Drug Combinations; D004797:Enzyme-Linked Immunosorbent Assay; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D007797:Laminin / Q000737:chemistry; D008027:Light; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D009361:Neoplasm Invasiveness; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology; D010777:Photochemistry / Q000379:methods; D011108:Polymers / Q000737:chemistry; D011509:Proteoglycans / Q000737:chemistry; D012235:Rhodamines / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013343:Styrenes / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors; D014014:Tissue Adhesives; D046509:Tissue Culture Techniques; D023822:Tissue Engineering / Q000379:methods*; D014945:Wound Healing
D000906:Antibodies; D000941:Antigens; D001672:Biocompatible Materials; D004396:Coloring Agents; D003470:Culture Media; D016207:Cytokines; D004338:Drug Combinations; D007797:Laminin; D011108:Polymers; D011509:Proteoglycans; D012235:Rhodamines; D013343:Styrenes; D014014:Tissue Adhesives; C059485:matrigel; D012710:Serum Albumin, Bovine; D005780:Gelatin; D003094:Collagen