Genomic cloning and expression of vitellogenin gene from the self-fertilizing fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae).
We cloned the vitellogenin gene from the self-fertilizing fish Rivulus marmoratus, and sequenced 12,326 bp. The number of exons of R. marmoratus and rainbow trout vitellogenin genes were different, and also the splicing junctions are different throughout most of the exons and introns but the amino acid similarity of R. marmoratus vitellogenin gene to other species was rather high. In promoter region of R. marmoratus vitellogenin gene, there were several E2 binding sites and the estrogen response element (ERE). We discuss here the gene structure and expression of R. marmoratus vitellogenin gene.
Kim
Il-Chan
IC
Department of Biochemistry, College of Medicine, Hanyang University, Seoul 133-791, Republic of Korea.
Chang
Sung Yeoul
SY
Williams
Tim D
TD
Ja Kim
Young
Y
Yoon
Yong-Dal
YD
Lee
Yong-Sung
YS
Park
Eun-Ho
EH
Lee
Jae-Seong
JS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003532:Cyprinodontiformes / Q000235:genetics*; D017931:DNA Primers; D040461:Gene Components; D015698:Genomic Library; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D020218:Response Elements / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014819:Vitellogenins / Q000235:genetics*
D017931:DNA Primers; D014819:Vitellogenins