TFF3 expression at the esophagogastric junction is increased in gastro-esophageal reflux disease (GERD).
At the gastric cardia, the molecular mechanisms of inflammation and metaplasia are incompletely understood. Thus, the aim of this study was to determine the expression of TFF1, TFF2 and TFF3 at this site and correlate these data with Helicobacter pylori infection or gastro-esophageal reflux disease (GERD). In 27 patients without intestinal metaplasia at the cardia, endoscopic biopsies were obtained for histology and RT-PCR. TFF1 and TFF2 were expressed in all cardia samples. TFF3 expression was significantly more frequent at the cardia (n = 15/24) than in the corpus (n = 2/26). TFF3 expression at the cardia was mainly observed in GERD patients, and there was a clear tendency towards higher interleukin-8 (IL-8) transcription levels; whereas TFF3 expression was not correlated with the H. pylori status or to tumor necrosis factor-alpha (TNF-alpha) expression. The expression of TFF3 at the cardia may represent an adaptation to GERD and precede the development of Barrett's esophagus.
Peitz
Ulrich
U
Department of Gastroenterology, Hepatology, and Infectiology, Otto-von-Guericke University, Leipziger Str. 44, D-39120 Magdeburg, Germany. ulrich.peitz@medizin.uni-magdeburg.de
Kouznetsova
Irina
I
Wex
Thomas
T
Gebert
Ingrid
I
Vieth
Michael
M
Roessner
Albert
A
Hoffmann
Werner
W
Malfertheiner
Peter
P
D001471:Barrett Esophagus / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D002299:Cardia / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D004943:Esophagogastric Junction / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D005764:Gastroesophageal Reflux / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D016481:Helicobacter Infections / Q000378:metabolism / Q000473:pathology; D016480:Helicobacter pylori / Q000378:metabolism; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D009077:Mucins / Q000378:metabolism*; D009124:Muscle Proteins / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D000071163:Trefoil Factor-1; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D025521:Tumor Suppressor Proteins
D016209:Interleukin-8; D009077:Mucins; D009124:Muscle Proteins; D010455:Peptides; D011506:Proteins; C051838:TFF1 protein, human; C000604007:TFF2 protein, human; C082495:TFF3 protein, human; C439524:TNF protein, human; D000071163:Trefoil Factor-1; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; D014409:Tumor Necrosis Factor-alpha; D025521:Tumor Suppressor Proteins