title: Cribriform-morular variant of papillary thyroid carcinoma. Report of a case showing morules with peculiar nuclear clearing.
abstract: BACKGROUND
  There have been only 4 reported cases of cribriform-morular variant of papillary thyroid carcinoma (CMVPTC) with cytologic findings from fine needle aspiration. In these reports, the cytologic findings do not fully reflect the histologic characteristics of this entity. We report a case of CMVPTC in which a cribriform pattern without colloid and epithelial morules with peculiar nuclear clearing (PNC) were present in smears, thus fulfilling the criteria for a cytologic diagnosis of CMVPTC. Protein truncation tests for APC molecule abnormality indicated the presence of germline mutation in the patient's APC gene.
  
  
  METHODS
  A 30-year-old woman had multiple thyroid tumors. Aspiration cytology revealed a large number of round to spindle-shaped atypical-cells showing sheet-like, cribriform, follicular, whorl-like and solid, 3-dimensional arrangements. The cribriform and follicular arrangements did not contain colloid in the lumen. The powdery chromatin pattern characteristic of papillary carcinoma was not observed, but there were scattered intranuclear cytoplasmic pseudoinclusions and grooved nuclei. The nuclei of the atypical cells presenting in the whorl formations showed enlargement, thickened nuclear membranes and entirely clear contents, consistent with PNC. Hyalinelike necrotic cells were also observed in the cell clusters or in the background. Histologic and immunohistochemical findings were typical of CMVPTC.
  
  
  CONCLUSIONS
  The cribriform pattern without colloid, fascicular or whorl formation of spindle cells, and morules with PNC are identifiable on cytologic smears and are sufficiently distinctive to allow a cytologic diagnosis of CMVPTC.
authors.affiliation: Department of Pathology, University of Tokushima School of Medicine, 3-18-15 Kuramoto-cho, Tokushima, 770-8503, Japan. skuma@basic.med.tokushima-u.ac.jp
authors.forename: Seiji
authors.initials: S
authors.lastname: Kuma
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Hirokawa
authors.forename: Bing
authors.initials: B
authors.lastname: Xu
authors.forename: Akira
authors.initials: A
authors.lastname: Miyauchi
authors.forename: Kennichi
authors.initials: K
authors.lastname: Kukudo
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Sano
mesh_terms: D000236:Adenoma / Q000473:pathology; D000328:Adult; D044963:Biopsy, Fine-Needle; D018276:Carcinoma, Medullary / Q000175:diagnosis; D002291:Carcinoma, Papillary / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D002467:Cell Nucleus / Q000473:pathology; D003598:Cytoskeletal Proteins / Q000032:analysis; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis; D019394:Ki-67 Antigen / Q000032:analysis; D018396:Mucin-1 / Q000378:metabolism; D013954:Thyroglobulin / Q000032:analysis; D013964:Thyroid Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D013965:Thyroidectomy; D015534:Trans-Activators / Q000032:analysis; D014463:Ultrasonography; D014746:Vimentin / Q000032:analysis; D051176:beta Catenin
chemical_list: C495270:CTNNB1 protein, human; D003598:Cytoskeletal Proteins; D019394:Ki-67 Antigen; D018396:Mucin-1; D015534:Trans-Activators; D014746:Vimentin; D051176:beta Catenin; D007633:Keratins; D013954:Thyroglobulin