title: Adjuvant can improve protection induced by OMV vaccine against Neisseria meningitidis serogroups B/C in neonatal mice.
abstract: Meningococcal outer membrane vesicle (OMV) vaccines are weak antigens in infants. This study aimed at investigating alternative adjuvants for induction of functional antibodies in newborn mice. Serogroup B/C anti-meningococcal vaccines, consisting of capsular polysaccharide from serogroup C (PSC) conjugated to OMV from one serogroup B serosubtype prevalent in Brazil, combined with OMV from another prevalent serosubtype, were tested in newborn and adult mice with the following adjuvants: aluminum hydroxide, MPL (monophosphoryl lipid A), Titermax and MF59. Total IgG, IgG avidity index determination and bactericidal assay were performed with sera from immunized mice. Antibodies induced against PSC in newborn mice showed avidity and bactericidal titers, similar to those obtained in adult mice, independently of the adjuvant. Evidence is presented that the inclusion of MF59 enhanced the immune response against OMV in newborn mice.
authors.affiliation: Centro de Biotecnologia, Instituto Butantan, Avenida Vital Brasil 1500, CEP 05503-900 São Paulo, Brazil.
authors.forename: Lucila O
authors.initials: LO
authors.lastname: Fukasawa
authors.forename: Waldely O
authors.initials: WO
authors.lastname: Dias
authors.forename: Rocilda P F
authors.initials: RP
authors.lastname: Schenkman
authors.forename: I
authors.initials: I
authors.lastname: Raw
authors.forename: Martha M
authors.initials: MM
authors.lastname: Tanizaki
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000831:Animals, Newborn; D000915:Antibody Affinity; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001770:Blood Bactericidal Activity; D004195:Disease Models, Animal; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008589:Meningococcal Infections / Q000382:microbiology / Q000517:prevention & control*; D022401:Meningococcal Vaccines / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D038541:Neisseria meningitidis, Serogroup B / Q000276:immunology*; D038542:Neisseria meningitidis, Serogroup C / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology; D011136:Polysorbates / Q000008:administration & dosage; D013185:Squalene / Q000008:administration & dosage; D018074:Vaccines, Conjugate / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D001425:Bacterial Outer Membrane Proteins; D007074:Immunoglobulin G; C089950:MF59 oil emulsion; D022401:Meningococcal Vaccines; D011135:Polysaccharides, Bacterial; D011136:Polysorbates; D018074:Vaccines, Conjugate; C037676:meningococcal group C polysaccharide; D013185:Squalene