title: TFG is a novel fusion partner of NOR1 in extraskeletal myxoid chondrosarcoma.
abstract: Extraskeletal myxoid chondrosarcoma (EMC) is characterized by recurrent chromosomal translocations resulting in fusions of the nuclear receptor gene NOR1 (also known as CHN or TEC) to various N-terminal partners, including EWS and TAF2N (or RBP56). Significant structural homology of EWS or TAF2N to TLS (or FUS) prompted us to investigate a potential novel gene fusion of NOR1 to TLS in EMCs without detectable known NOR1 fusions. In one of the EMCs examined, our reverse-transcription polymerase chain reaction using NOR1 and TLS primers unexpectedly amplified a cDNA sequence derived not from a TLS/NOR1 fusion but from a TFG/NOR1 fusion, a hitherto undescribed fusion type in EMC, probably a result of incidental misannealing by the TLS primer, which has a sequence partially identical to TFG. Encoding a protein with a putative coiled-coil structure, TFG previously was identified by a homology search in the Expressed Sequence Tag Database as having an SPYGQ-rich region similar to the N-terminal parts of EWS and TLS. TFG/NOR1 fusion appears to play an oncogenic role equivalent to those of other NOR1 fusions in EMC.
authors.affiliation: Department of Pathology and Oncology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Hisaoka
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Ishida
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Imamura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hashimoto
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D001483:Base Sequence / Q000235:genetics; D002813:Chondrosarcoma / Q000235:genetics*; D006801:Humans; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D012983:Soft Tissue Neoplasms / Q000235:genetics*; D033921:Vesicular Transport Proteins
chemical_list: D026901:Membrane Transport Proteins; D015514:Oncogene Proteins, Fusion; D011506:Proteins; C479458:OSCP1 protein, human; C096163:TFG protein, human; D033921:Vesicular Transport Proteins