[Effect of blockers of NO-synthase, NO donors, and exogenous E2 and F2alpha prostaglandins on mouse oocytes].
The effects of nitric oxide (NO) synthase (NOS) blocker (L-NMMA), NO donor (sodium nitroprusside, Na-NP), and exogenous prostaglandins (PGs) F2 alpha and E2 on the meiotic maturation (MM) of murine oocytes were studied. The exogenous PGs in a dose of 3.00 micrograms/kg inhibited the MM of oocytes. The effect of L-NMMA on the MM of oocytes was studied for the first time depending on the size of a follicle from which they were isolated. The maximum inhibition was observed for oocytes isolated from small follicles. Na-NP induced an increase in the number of oocytes from small and medium follicles, capable of forming polar bodies after a 20-h cultivation. The introduction of PG F2 alpha to the test animals pretreated with L-NMMA potentiated the inhibition of MM. The NO donor decreased the inhibiting effect of PG F2 alpha on the MM of oocytes isolated from small and medium follicles. The results confirm that NO/NOS ovarian system is involved into the mechanism of the PG action on the MM of murine oocytes and, probably, in the general mechanism of PG activity.
Voznesenskaia
T Iu
TIu
Immunology and Cytotoxic Sera Department, Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, ul. Bogomol'tsa 4, Kiev, Ukraine.
Blashkiv
T V
TV
D000818:Animals; D015237:Dinoprost / Q000494:pharmacology*; D015232:Dinoprostone / Q000494:pharmacology*; D005260:Female; D008540:Meiosis / Q000187:drug effects; D051379:Mice; D008808:Mice, Inbred CBA; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
D020030:Nitric Oxide Donors; D015237:Dinoprost; D019001:Nitric Oxide Synthase; D015232:Dinoprostone