Features of dnaK operon genes of the obligate thermophile Bacillus thermoglucosidasius KP1006.
The dnaK gene was cloned from the obligate thermophile Bacillus thermoglucosidasius KP1006, together with the grpE and dnaJ genes in the same operon. The dnaK, grpE and dnaJ genes showed high identity with those of other bacterial strains, particularly with those of Bacillus stearothermophilus NUB36, despite an extremely low homology for the corresponding total genomic DNA. There were significant differences in the proline content of the DnaK operon proteins which is closely correlated with the thermostability of enzyme proteins. The proline content was higher in the GrpE, DnaK and DnaJ proteins of the thermophilic as opposed to the mesophilic strains. The overexpression of the B. thermoglucosidasius DnaK protein in Escherichia coli MV1184 results in extreme filamentation without inhibition on cell growth. The B. thermoglucosidasius DnaK protein seemed to exclusively disturb septation in E. coli cells which suggests that it interacts with key protein(s) involved in cell septation.
Watanabe
K
K
Department of Applied Biochemistry, Kyoto Prefectural University, Shimogamo, Sakyo, Kyoto 606-8522, Japan.
Iwashiro
T
T
Suzuki
Y
Y
D000251:Adenosine Triphosphatases / Q000235:genetics*; D001407:Bacillus / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics*; D002455:Cell Division; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004795:Enzyme Stability / Q000235:genetics; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000235:genetics; D018832:Molecular Chaperones / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon; D011392:Proline / Q000032:analysis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C494635:DnaJ protein, E coli; D029968:Escherichia coli Proteins; C068113:GrpE protein, Bacteria; C489400:GrpE protein, E coli; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D011994:Recombinant Proteins; D011392:Proline; D000251:Adenosine Triphosphatases; C115251:DnaK protein, Brevibacillus choshinensis