Aggregation and G-quadruplex DNA-binding study of 6a,12a-diazadibenzo-[a,g]fluorenylium derivative.
The aggregation and DNA binding behavior of a new G-quadruplex selective ligand, 6a,12a-diazadibenzo-[a,g]fluorenylium derivative, was studied by UV-vis absorption and fluorescence spectroscopy. The formation of ligand aggregates with different spectral characteristics was observed at low and high concentration of NaCl, respectively. The ligand binds to G-quadruplex with much higher affinity than to single- and double-stranded DNA.
Juskowiak
Bernard
B
Faculty of Chemistry, A. Mickiewicz University, 60-780 Poznan, Poland. juskowia@amu.edu.pl
Galezowska
Elzbieta
E
Koczorowska
Natalia
N
Hermann
Tadeusz W
TW
D001372:Aza Compounds / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D005449:Fluorenes / Q000737:chemistry / Q000378:metabolism*; D005456:Fluorescent Dyes; D054856:G-Quadruplexes; D006147:Guanine / Q000737:chemistry*; D008024:Ligands; D009690:Nucleic Acid Conformation; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D001372:Aza Compounds; D004277:DNA, Single-Stranded; D005449:Fluorenes; D005456:Fluorescent Dyes; D008024:Ligands; D006147:Guanine; D004247:DNA