title: Schwann cell seeded guidance tubes restore erectile function after ablation of cavernous nerves in rats.
abstract: OBJECTIVE
  Dissection of the cavernous nerves eliminates spontaneous erections. We evaluated the ability of Schwann cell seeded nerve guidance tubes to restore erections after bilateral cavernous nerve resection in rats.
  
  
  METHODS
  Sections (5 mm) of the cavernous nerve were excised bilaterally, followed by immediate bilateral microsurgical reconstruction. In 10 animals per group (20 study nerves) reconstruction was performed by genitofemoral nerve interposition, interposition of silicone tubes or interposition of silicone tubes seeded with homologous Schwann cells. As the control 10 animals (20 study nerves) underwent sham operation (positive control) and bilateral nerve ablation (without reconstruction) was performed in a further 10 (negative control). Erectile function was evaluated 3 months postoperatively by relaparotomy, electrical nerve stimulation and intracavernous pressure recording.
  
  
  RESULTS
  After 3 months neurostimulation resulted in an intact erectile response in 90% (18 of 20) of Schwann cell grafts, while treatment with autologous nerves (30% or 6 of 20) or tubes only (50% or 10 of 20) was less successful (p <0.01). Whereas untreated ablated rats showed no inducible erections (0% or 0 of 20), all sham operated animals had an intact erectile response (100% or 20 of 20). Maximum intracavernous pressure upon electrostimulation was significantly elevated using Schwann cell grafts compared to results in the other treatment groups (p <0.001). Morphological evaluation revealed advanced regeneration within Schwann cell grafts.
  
  
  CONCLUSIONS
  Schwann cell seeded guidance tubes restore erectile function after the ablation of cavernous nerves in rats and they are superior to autologous nerve grafts.
authors.affiliation: Department of Urology, Technische Universität, Munich, Germany. mayflorian@aol.com
authors.forename: F
authors.initials: F
authors.lastname: May
authors.forename: N
authors.initials: N
authors.lastname: Weidner
authors.forename: K
authors.initials: K
authors.lastname: Matiasek
authors.forename: C
authors.initials: C
authors.lastname: Caspers
authors.forename: T
authors.initials: T
authors.lastname: Mrva
authors.forename: M
authors.initials: M
authors.lastname: Vroemen
authors.forename: J
authors.initials: J
authors.lastname: Henke
authors.forename: A
authors.initials: A
authors.lastname: Lehmer
authors.forename: H
authors.initials: H
authors.lastname: Schwaibold
authors.forename: W
authors.initials: W
authors.lastname: Erhardt
authors.forename: B
authors.initials: B
authors.lastname: Gänsbacher
authors.forename: R
authors.initials: R
authors.lastname: Hartung
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D002478:Cells, Cultured; D004558:Electric Stimulation; D008297:Male; D009416:Nerve Regeneration / Q000502:physiology*; D010410:Penile Erection / Q000502:physiology*; D010413:Penis / Q000294:innervation*; D051381:Rats; D011916:Rats, Inbred F344; D012583:Schwann Cells / Q000502:physiology*