Cloning and heterologous expression of a novel insecticidal gene (tccC1) from Xenorhabdus nematophilus strain.
We have identified and cloned a novel toxin gene (tccC1/xptB1) from Xenorhabdus nematophilus strain isolated from Korea-specific entomophagous nematode Steinernema glaseri MK. The DNA sequence of cloned toxin gene (3048 bp) has an open reading frame encoding 1016 amino acids with a predicted molecular mass of 111058 Da. The toxin sequence shares 50-96% identical amino acid residues with the previously reported tccC1 cloned from X. nematophilus, Photorhabdus luminescens W14 P. luminescens TTO1, and Yersinia pestis CO92. The toxin gene was successfully expressed in Escherichia coli, and the recombinant toxin protein caused a rapid cessation in mortality of Galleria mellonella larvae (80% death of larvae within 2 days). Conclusively, the heterologous expression of the novel gene tccC1 cloned into E. coli plasmid vector produced recombinant toxin with high insecticidal activity.
Joo Lee
Pom
P
Department of Chemical and Biological Engineering, Korea University, Anam-Dong 5-1, Sungbuk-Ku, Seoul 136-701, South Korea.
Ahn
Ji-Young
JY
Kim
Yang-Hoon
YH
Wook Kim
Seung
S
Kim
Ji-Yeon
JY
Park
Jae-Sung
JS
Lee
Jeewon
J
D000595:Amino Acid Sequence; D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics* / Q000378:metabolism* / Q000494:pharmacology; D003001:Cloning, Molecular; D007814:Larva / Q000187:drug effects; D008969:Molecular Sequence Data; D009036:Moths / Q000187:drug effects; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D020593:Xenorhabdus / Q000235:genetics* / Q000378:metabolism*
D001427:Bacterial Toxins; D011994:Recombinant Proteins