The modulating effects of quercetin and rutin on the mitomycin C induced DNA damage.
The present study was carried out to investigate the modulating effects of the two flavonoids quercetin and rutin on the mutagenic anticancer drug mitomycin C by single cell gel electrophoresis (Comet assay) in human lymphocytes. Lymphocytes were incubated with different concentrations of quercetin and rutin, with or without mitomycin C, and DNA damage was evaluated. Concentrations of 0.03, 0.15, 0.3, 0.6, 1.5 and 3mM quercetin significantly reduced the DNA strand breakage induced by mitomycin C (P<0.001) but the highest concentration of 6mM quercetin did not show a protective effect. The frequency of damaged cells induced by mitomycin C was not changed at 0.02 mM, and also at the highest concentrations of 1.64 and 3.28 mM rutin. However, at concentrations of 0.08, 0.16, 0.33 and 0.82 mM rutin cells were protected from DNA damage. Thus, in human lymphocytes quercetin and rutin displayed protective effects on DNA damage induced by mitomycin C, in a concentration-dependent manner.
Undeğer
Ulkü
U
Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Hacettepe University, TR-06100 Ankara, Turkey.
Aydin
Sevtap
S
Başaran
A Ahmet
AA
Başaran
Nurşen
N
D000903:Antibiotics, Antineoplastic / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D020552:Comet Assay; D004249:DNA Damage; D004347:Drug Interactions; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology; D008875:Middle Aged; D016685:Mitomycin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology*; D012431:Rutin / Q000494:pharmacology*
D000903:Antibiotics, Antineoplastic; D000975:Antioxidants; D016685:Mitomycin; D012431:Rutin; D011794:Quercetin