Activation of NF-kappaB/Rel transcription factors in human primary peripheral blood mononuclear cells by interleukin 7.
Pathways that regulate the activation of NF-kappaB/Rel transcription factors are known to include signaling through a number of cytokine receptors. Interleukin 7 (IL-7), produced by bone marrow and other stromal cells, is a key factor for differentiation and survival in the lymphoid and other compartments. We found that human recombinant IL-7 induced NF-kappaB/Rel activation, analyzed by electrophoretic mobility shift assay (EMSA), in human peripheral blood T lymphocytes from healthy donors. Induced complexes included p65 and p50 NF-kappaB/Rel subunits. These results demonstrate for the first time that IL-7 can participate in signaling leading to NF-kappaB/Rel activation.
Lamberti
Annalisa
A
Department of Biochemistry and Biomedical Technologies, University Federico II, via Pansini 5, I-80131 Naples, Italy.
Petrella
Antonello
A
Pascale
Maria
M
Romano
Maria Fiammetta
MF
Bisogni
Rita
R
Vita
Natalio
N
Venuta
Salvatore
S
Turco
Maria Caterina
MC
D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015851:Interleukin-7 / Q000235:genetics / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008213:Lymphocyte Activation; D016328:NF-kappa B / Q000378:metabolism*; D020569:Proto-Oncogene Proteins c-rel / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism
D015851:Interleukin-7; D016328:NF-kappa B; D020569:Proto-Oncogene Proteins c-rel; D011994:Recombinant Proteins; D014157:Transcription Factors