Detoxification of amitriptyline by oligochitosan derivatives.
Oligo-chitosans were chemically modified with dinitrophenyl groups for selective and rapid adsorption of amitriptyline by forming pi-pi complexes. 1H-NMR was utilized not only for characterization of modified chitosans but also for monitoring the aromatic-aromatic interaction. The variation in the chemical shift of aromatic protons was followed to monitor the aromatic-aromatic interaction. Upfield shift of aromatic protons of dinitrophenyl groups supports aromatic-aromatic interactions with amitriptyline. Drug uptake test by HPLC reveals that dinitrophenyl chitosan particles (1-2 microm) at 0.4 wt% (w/v) in a saline solution (pH 6.9) adsorb 90% amitriptyline within 30 min.
Lee
Dong-Won
DW
Department of Materials Science and Engineering, University of Florida, Gainesville, FL 32611, USA.
Baney
Ronald
R
D018759:Adrenergic Uptake Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D000327:Adsorption; D000639:Amitriptyline / Q000737:chemistry / Q000494:pharmacology*; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D008658:Inactivation, Metabolic; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D009844:Oligosaccharides; D011522:Protons; D013696:Temperature; D013997:Time Factors
D018759:Adrenergic Uptake Inhibitors; D009844:Oligosaccharides; D011522:Protons; C493484:oligochitosan; D002686:Chitin; D000639:Amitriptyline; D048271:Chitosan