title: [The mechanism for up-regulation of HLA-I expression on HepG2 cells by HBV].
abstract: OBJECTIVE
  To explore the mechanism of up-regulation of HLA-I expression on HepG2 cells by wild type (WT) and nucleocapsid mutants(L97 and V60) of hepatitis B virus (HBV).
  
  
  METHODS
  The HBV-stable expression vectors EBO-WT, EBO-L97 and EBO-V60 were transfected into HepG2 cells via the liposome mediation, respectively. The cells were assayed by semi-quantitative RT-PCR for HLA-A gene and antigen presentation-related genes LMP2, TAP1, and tapasin mRNA expression. Western blot was applied for analysis of HLA-I protein expression in the cells.
  
  
  RESULTS
  HepG2 cells transfected by the 3 HBV expression vectors expressed HLA-A and TAP1, while there was no expression of HLA-A and only marginal expression of TAP1 in HepG2 cells transfected by control vector EBO. The expression level of HLA-A in the transfected cells decreased successively in the order of EBO-L97, EBO-WT and EBO-V60. There was no significant difference in the expression level of TAP1 between HepG2 cells transfected by the 3 HBV expression vectors. No detectable expression of LMP2 and tapasin was observed for all transfected cells.
  
  
  CONCLUSIONS
  HBV can induce the expression of HLA-I molecule and TAP1 in HepG2 cells.
authors.affiliation: Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. chenwhong101@163.com
authors.forename: Wei-hong
authors.initials: WH
authors.lastname: Chen
authors.forename: Jin
authors.initials: J
authors.lastname: Ding
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Gong
authors.forename: Jiu-ping
authors.initials: JP
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Pan
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Sha
authors.initials: S
authors.lastname: Hong
authors.forename: Yong-xing
authors.initials: YX
authors.lastname: Zhou
mesh_terms: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D002460:Cell Line; D005786:Gene Expression Regulation; D015234:HLA-A Antigens / Q000235:genetics; D006515:Hepatitis B virus / Q000502:physiology*; D015395:Histocompatibility Antigens Class I / Q000032:analysis / Q000235:genetics*; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D014162:Transfection; D015854:Up-Regulation
chemical_list: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters; D015234:HLA-A Antigens; D015395:Histocompatibility Antigens Class I; D012333:RNA, Messenger; C073119:TAP1 protein, human