title: [Preparation and characterization of monoclonal antibodies against recombinant human NDPK-A].
abstract: OBJECTIVE
  To prepare monoclonal antibodies (mAb) against recombinant human nucleoside diphosphate kinase-A(NDPK-A) and characterize their properties.
  
  
  METHODS
  BALB/c mice were immunized with rhNDPK-A, and mAb was prepared by hybridoma technique. Ig subclass and specificity was analysed by double immunodiffusion and western blot respectively. The titres of mAbs in ascitic fluid, relative affinity and epitopes recognized by mAbs were determined by indirect ELISA.
  
  
  RESULTS
  6 hybridoma cell lines secreting anti-rhNDPK-A mAbs were obtained. Their Ig subclass belonged to IgG1. The titers of 6 mAbs in ascitic fluid were 1x10(-4)-5x10(-6). Relative affinity of mAbs were 4.5x10(-9)-2.8x10(-10) mol/L. They recognized 3 different epitopes on rhNDPK-A molecule.
  
  
  CONCLUSIONS
  6 mAbs against rhNDPK-A have been prepared successfully which provide useful reagent for clinical diagnosis and further research.
authors.affiliation: Biomedicine Research & Development Center of Jinan University, Guangzhou 510632, China.
authors.forename: Xue-ling
authors.initials: XL
authors.lastname: Li
authors.forename: Yi-fei
authors.initials: YF
authors.lastname: Wang
authors.forename: Sheng
authors.initials: S
authors.lastname: Xiong
authors.forename: Mei-ying
authors.initials: MY
authors.lastname: Zhang
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Jiu-xiang
authors.initials: JX
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Zuo-ya
authors.initials: ZY
authors.lastname: Zheng
authors.forename: Cong-zhu
authors.initials: CZ
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase; D011506:Proteins / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000911:Antibodies, Monoclonal; D054778:NM23 Nucleoside Diphosphate Kinases; D011506:Proteins; D011994:Recombinant Proteins; C517939:Nme1 protein, mouse; D009701:Nucleoside-Diphosphate Kinase