title: [The influence of TLSFJM on the proportion of Th1/Th2-like cell subsets].
abstract: OBJECTIVE
  To explore the influence of TLSF(JM) on the proportion of alloantigen activated Th1 and Th2-like cell subsets.
  
  
  METHODS
  TLSF(JM) or IL-4 was added to mixed lymocyte reaction(MLR) system. The influence of TLSF(JM) on the proportion of Th1 and Th2-like cell subsets was analyzed by intracellular immunofluorescence staining and FACS.
  
  
  RESULTS
  In the TLSF(JM) group, the proportion of IFN-gamma(+) cells differentiated from activated lymphoblast descended from 49.8% to 43.1%, IL-4(+) cells from 75.4% to 43.7% and IL-6(+) cells from 67.8% to 52.6%. The similar tendency was also observed in the unactivated small lymphocytes.
  
  
  CONCLUSIONS
  TLSF(JM) can inhibit both the Th1 and Th2-like cell subsets, but mainly inhibit the Th2-like cell subset, thereby reducing the proportion of Th2-like cell subsets.
authors.affiliation: Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Wei-ning
authors.initials: WN
authors.lastname: Han
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yun-xin
authors.initials: YX
authors.lastname: Cao
authors.forename: Xin
authors.initials: X
authors.lastname: Xie
authors.forename: Bo-quan
authors.initials: BQ
authors.lastname: Jin
mesh_terms: D045744:Cell Line, Tumor; D017077:Culture Media, Conditioned; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D015847:Interleukin-4 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000473:pathology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D016393:Lymphoma, B-Cell / Q000473:pathology; D013491:Suppressor Factors, Immunologic / Q000302:isolation & purification / Q000494:pharmacology*; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D017077:Culture Media, Conditioned; D015850:Interleukin-6; D013491:Suppressor Factors, Immunologic; D015847:Interleukin-4; D007371:Interferon-gamma