Effect of Iloprost on endothelin-1-induced free radical activation in rabbit brain stem.
Iloprost, a stable analogue of prostacyclin, was used to reverse the early period of vasoconstriction provoked by Endothelin-1 by administering into the rabbit basilar artery. We observed if this produced an effect on the central nervous system parenchyma mediated by free radical system. The red neurons were counted in brain stem sections stained with haematoxylin and eosin, while superoxide dismutase and malondialdehyde levels were measured in brain stem tissue samples as a marker of reactive oxygen metabolites; both 30 and 90 min after administration of either Endothelin-1 (0.25 ng) alone or Endothelin-1 followed by Iloprost (0.5 microg/kg) into the basilar artery. Endothelin-1 significantly increased the number of red neurons, while Iloprost significantly reduced them after 30 and 90 min. However, regarding the reactive oxygen metabolites; a similar reversing effect of Iloprost was not observed although superoxide dismutase levels were significantly decreased after Endothelin-1 infusion.
Keskil
I Semih
IS
Department of Neurosurgery, Ky?ry?kkale University School of Medicine Hospital, Kỳrỳkkale 71100, Turkey. sk06-k@tr.net
Kemali Baykaner
M
M
Aricioğlu
Aysel
A
Kaymaz
Memduh
M
Uysal
S Selçuk
SS
Ataoğlu
O Omür
OO
Balabanli
Barbaros
B
Ercan
Sevim
S
Ceviker
Necdet
N
D000818:Animals; D001488:Basilar Artery; D001923:Brain Chemistry; D001933:Brain Stem / Q000378:metabolism*; D019332:Endothelin-1 / Q000008:administration & dosage*; D005609:Free Radicals / Q000378:metabolism*; D016285:Iloprost / Q000008:administration & dosage*; D007269:Injections, Intra-Arterial; D008315:Malondialdehyde / Q000032:analysis; D009474:Neurons / Q000378:metabolism; D011817:Rabbits; D013482:Superoxide Dismutase / Q000032:analysis; D014661:Vasoconstriction / Q000187:drug effects*; D014665:Vasodilator Agents / Q000008:administration & dosage*
D019332:Endothelin-1; D005609:Free Radicals; D014665:Vasodilator Agents; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D016285:Iloprost