Isolation of photosystem II-enriched membranes and the oxygen-evolving complex subunit proteins from higher plants.
Methods for the isolation of highly active oxygen-evolving photosystem (PS)II membranes from higher plants and the purification of the oxygen-evolving complex (OEC) subunits are described. Membrane samples used as the material for various in vitro studies of PSII are prepared by solubilization of thylakoid membranes with the non-ionic detergent Triton X-100. The OEC subunit proteins are dissociated from the PSII-enriched membranes by alkaline treatment or salt treatment, and then purified by ion-exchange chromatography using an automated high-performance liquid chromatography system.
Yamamoto
Yasusi
Y
Graduate School of Natural Science and Technology, Okayama University, Japan.
Sakuma
Shinsuke
S
Shen
Jian-Ren
JR
D002021:Buffers; D002845:Chromatography / Q000379:methods; D003902:Detergents / Q000737:chemistry; D007425:Intracellular Membranes / Q000737:chemistry* / Q000378:metabolism; D046911:Macromolecular Substances; D017830:Octoxynol / Q000737:chemistry; D010100:Oxygen / Q000378:metabolism*; D018532:Pisum sativum / Q000737:chemistry; D045332:Photosystem II Protein Complex / Q000737:chemistry* / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry*; D021122:Protein Subunits / Q000737:chemistry*; D018724:Spinacia oleracea / Q000737:chemistry
D002021:Buffers; D003902:Detergents; D046911:Macromolecular Substances; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D021122:Protein Subunits; D017830:Octoxynol; D010100:Oxygen