Cloning and identification of NS5ATP2 gene and its spliced variant transactivated by hepatitis C virus non-structural protein 5A.
OBJECTIVE
To clone, identify and study new NS5ATP2 gene and its spliced variant transactivated by hepatitis C virus non-structural protein 5A.


METHODS
On the basis of subtractive cDNA library of genes transactivated by NS5A protein of hepatitis C virus, the coding sequence of new gene and its spliced variant were obtained by bioinformatics method. Polymerase chain reaction (PCR) was conducted to amplify NS5ATP2 gene.


RESULTS
The coding sequence of a new gene and its spliced variant were cloned and identified successfully.


CONCLUSIONS
A new gene has been recognized as the new target transactivated by HCV NS5A protein. These results brought some new clues for studying the biological functions of new genes and pathogenesis of the viral proteins.
Yang
Qian
Q
Department of Infectious Diseases, First Hospital of Xi'an Jiaotong University, Shaanxi Province, China.
Cheng
Jun
J
Liu
Yan
Y
Hong
Yuan
Y
Wang
Jian-Jun
JJ
Zhang
Shu-Lin
SL
D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D001483:Base Sequence; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D015967:Gene Expression Regulation, Viral; D015723:Gene Library; D016174:Hepacivirus / Q000235:genetics*; D018197:Hepatoblastoma; D006801:Humans; D008113:Liver Neoplasms; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012326:RNA Splicing; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000378:metabolism; D012324:RNA-Dependent RNA Polymerase
D000952:Antigens, Polyomavirus Transforming; D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase