ARChip epoxy and ARChip UV for covalent on-chip immobilization of pmoA gene-specific oligonucleotides.
ARChip Epoxy and ARChip UV are presented as novel chip platforms for oligonucleotide immobilization. ARChip Epoxy is made of reactive epoxy resin available commercially. ARChip UV consists of photoactivatable poly(styrene-co-4-vinylbenzylthiocyanate). Both ARChip surfaces are tested in a model assay based on oligonucleotide probes from a real-life genotyping project and are evaluated in comparison with five commercial chip surfaces based on nitrocellulose, epoxy, and aldehyde polymer, and two different aminosilanes. Optimum print buffer, spotter compatibility, and data normalization are discussed.
Preininger
Claudia
C
Division of Environmental and Life Sciences, ARC Seibersdorf Research GmbH, Biotechnology Unit, A-2444 Seibersdorf, Austria. claudia.preininger@arcs.ac.at
Bodrossy
Levente
L
Sauer
Ursula
U
Pichler
Rudolf
R
Weilharter
Alexandra
A
D001419:Bacteria / Q000235:genetics*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004853:Epoxy Resins / Q000737:chemistry*; D046228:Microarray Analysis / Q000379:methods*; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry*; D010105:Oxygenases / Q000235:genetics*; D011137:Polystyrenes / Q000737:chemistry*
D003432:Cross-Linking Reagents; D004853:Epoxy Resins; D009841:Oligonucleotides; D011137:Polystyrenes; D010105:Oxygenases; C021555:methane monooxygenase