title: Proteomic characterization of novel serum amyloid P component variants from human plasma and urine.
abstract: Serum amyloid P component (SAP) is a human plasma protein that has been widely studied for its influence on amyloid plaque formation and stabilization. SAP was characterized directly from human plasma and urine samples via novel affinity mass spectrometry-based proteomic technology that is able to readily discriminate between mass-altered protein variants. These analyses were able to identify several variants of SAP that have not been previously reported. These variants include microheterogeneity of the glycan structure, from the loss of one or both terminal sialic acid residues, as well as the loss of the C-terminal valine residue. Moreover, the analysis of urine allowed for the consistent identification of serum amyloid P component as a normal constituent of the urine proteome.
authors.affiliation: Intrinsic Bioprobes Inc., Tempe, AZ 85281, USA. ukiernan@intrinsicbio.com
authors.forename: Urban A
authors.initials: UA
authors.lastname: Kiernan
authors.forename: Dobrin
authors.initials: D
authors.lastname: Nedelkov
authors.forename: Kemmons A
authors.initials: KA
authors.lastname: Tubbs
authors.forename: Eric E
authors.initials: EE
authors.lastname: Niederkofler
authors.forename: Randball W
authors.initials: RW
authors.lastname: Nelson
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D014644:Genetic Variation; D006801:Humans; D007118:Immunoassay; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D008969:Molecular Sequence Data; D010949:Plasma / Q000737:chemistry*; D020543:Proteome; D000683:Serum Amyloid P-Component / Q000737:chemistry* / Q000235:genetics / Q000652:urine*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020543:Proteome; D000683:Serum Amyloid P-Component