Expression and characterization of Saccharomyces cerevisiae Cne1p, a calnexin homologue.
The calnexin homologue (Cne1p) of Saccharomyces cerevisiae was expressed in Escherichia coli to evaluate its chaperone function. The chaperone function was examined as to the effects on the suppression of thermal denaturation and the enhancement of refolding, using citrate synthase (CS) as a nonspecific chaperone substrate. Cne1p effectively suppressed the thermal denaturation of CS and enhanced the refolding of thermally or chemically denatured CS in a concentration-dependent manner. In addition, the chaperone function of Cne1p was greatly affected in the presence of monoglucosylated oligosaccharides (G1M9) that specifically bind to the lectin site. These results indicated that Cne1p functions as a molecular chaperone in Saccharomyces cerevisiae.
Xu
Xiaohua
X
Department of Biological Chemistry, Yamaguchi University, Yamaguchi 753-8515.
Kanbara
Kunitoshi
K
Azakami
Hiroyuki
H
Kato
Akio
A
D037281:Calnexin / Q000378:metabolism; D002950:Citrate (si)-Synthase / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006358:Hot Temperature; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry; D018832:Molecular Chaperones / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry; D011485:Protein Binding; D017510:Protein Folding; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000737:chemistry; D013997:Time Factors
C080582:CNE1 protein, S cerevisiae; D008565:Membrane Proteins; D018832:Molecular Chaperones; D009844:Oligosaccharides; D029701:Saccharomyces cerevisiae Proteins; D037281:Calnexin; D002950:Citrate (si)-Synthase; D005982:Glutathione Transferase