title: Differences between betaproteobacterial ammonia-oxidizing communities in marine sediments and those in overlying water.
abstract: To assess links between betaproteobacterial ammonia-oxidizing bacteria (AOB) in marine sediment and in overlying water, communities in Loch Duich, Scotland, were characterized by analysis of clone libraries and denaturant gradient gel electrophoresis of 16S rRNA gene fragments. Nitrosospira cluster 1-like sequences were isolated from both environments, but different sequence types dominated water and sediment samples. Detailed phylogenetic analysis of marine Nitrosospira cluster 1-like sequences in Loch Duich and surrounding regions suggests the existence of at least two different phylogenetic subgroups, potentially indicative of new lineages within the betaproteobacterial AOB, representing different marine ecotypes.
authors.affiliation: Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, United Kingdom.
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Freitag
authors.forename: James I
authors.initials: JI
authors.lastname: Prosser
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D020562:Betaproteobacteria / Q000145:classification* / Q000235:genetics; D017753:Ecosystem; D004586:Electrophoresis / Q000379:methods; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012606:Scotland; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D012336:RNA, Ribosomal, 16S; D000641:Ammonia