Comprehensive gene expression analysis of the response to straight-chain alcohols in Saccharomyces cerevisiae using cDNA microarray.
OBJECTIVE
The purpose of this study was to examine the gene expression profiles of yeast Saccharomyces cerevisiae subjected to straight-chain alcohols.


RESULTS
Lipophilic alcohols with high log Pow values were more toxic to yeast than those with low log Pow values. Morphological changes after exposure to ethanol, 1-pentanol, 1-octanol were observed, whereas n-pentane as a model hydrocarbon affected the surface of the outer membrane, with little change in organelles. Using cDNA microarrays, quite a few up-regulated gene categories were classified into the category 'cell rescue, defence and virulence' by ethanol, and the category 'energy' and 'metabolism' by 1-pentanol. Meanwhile, the characteristic genes up-regulated by n-pentane were not observed, and the expression profile was distantly related to ethanol, 1-pentanol and 1-octanol.


CONCLUSIONS
This study suggests that gene expression profiles at the whole genome level were intimately associated with the cell growth inhibition and morphological changes by straight-chain alcohols with differing log Pow values.


CONCLUSIONS
The study of comprehensive gene expression profiles by cDNA microarrays elucidates the straight-chain alcohol adaptation mechanisms.
Fujita
K
K
Japan Bioindustry Association (JBA), Daicel Chemical Industries, Ltd, Branch, Tsukuba, Japan. ka-fujita@aist.go.jp
Matsuyama
A
A
Kobayashi
Y
Y
Iwahashi
H
H
D020003:1-Octanol / Q000494:pharmacology; D000438:Alcohols / Q000494:pharmacology*; D016172:DNA Fingerprinting; D018076:DNA, Complementary / Q000032:analysis*; D004271:DNA, Fungal / Q000032:analysis*; D000431:Ethanol / Q000494:pharmacology; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis; D000439:Pentanols / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*
D000438:Alcohols; D018076:DNA, Complementary; D004271:DNA, Fungal; D000439:Pentanols; D000431:Ethanol; C024999:n-pentanol; D020003:1-Octanol