title: Laser spectral characterization in multiphoton microscopy.
abstract: Spectral and temporal characterization is a fundamental task when a tunable Ti:sapphire ultrafast laser system is operated for multiphoton microscopy applications. In the present paper simple procedures are reported that perform laser-peak-emission wavelength and bandwidth measurements without the need of any further instrumentation but a simple and inexpensive diffraction grating, by taking advantage of the confocal microscope imaging capabilities.
authors.affiliation: Istituto Nazionale di Ottica Applicata, Laboratorio di Biofotonica, Largo Enrico Fermi 6, 50125 Firenze, Italy. quercioli@ino.it
authors.forename: Franco
authors.initials: F
authors.lastname: Quercioli
authors.forename: Bruno
authors.initials: B
authors.lastname: Tiribilli
authors.forename: Massimo
authors.initials: M
authors.lastname: Vassalli
authors.forename: Alessandro
authors.initials: A
authors.lastname: Ghirelli
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods*; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods*; D012031:Refractometry / Q000295:instrumentation* / Q000706:statistics & numerical data*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity