[The increase in the variability of microsatellite-associated repeats in the genome of gamma-irradiated male mice is tissue specific].
The arbitrarily primed polymerase chain reaction (AP-PCR) was used to measure the level of polymorphism of microsatellite (MCS)-associated repeating sequences of spleen, lung, and brain DNA in the F1 progeny of male BALB/c mice exposed to acute gamma-radiation at doses of 50 cGy and 200 cGy 15 days before mating with unirradiated females. The variability of MCS-associated sequences in the genome of brain and lung cells was higher as compared to the spleen cells of the progeny of unirradiated males. In the progeny of irradiated males, a 20% increase in MCS polymorphism of spleen DNA was found as an increase in the frequency of "non-parent" bands in DNA-fingerprints as against to the progeny of unirradiated males. Significant changes in this parameter were revealed for brain tissue and not for lung tissue only in the progeny of males exposed to 200 cGy. The results suggest a tissue-specific character of transmission of radiation-induced alterations in the genome of germ cells of male parents to the somatic cells of the progeny.
Bezlepkin
V G
VG
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, 142290 Russia. bezlepkin@iteb.ru
Lomaeva
M G
MG
Vasil'eva
G V
GV
Sirota
N P
NP
Shaĭkhaev
G O
GO
Gaziev
A I
AI
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000528:radiation effects* / Q000648:ultrastructure; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays; D016678:Genome; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018895:Microsatellite Repeats / Q000235:genetics / Q000528:radiation effects*; D009928:Organ Specificity; D018812:Paternal Exposure; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic