Surfactant-aided size exclusion chromatography.
The flexibility and selectivity of size exclusion chromatography (SEC) for protein purification can be modified by adding non-ionic micelle-forming surfactants to the mobile phase. The micelles exclude proteins from a liquid phase similar to the exclusion effect of the polymer fibers of the size exclusion resin. This surfactant-aided size exclusion chromatography technology (SASEC) is demonstrated on the separation of two model proteins; bovine serum albumin (BSA) and myoglobin (Myo). The effect of the added surfactants on the distribution behavior of the proteins is predicted adequately by a size exclusion model presented in this paper.
Horneman
D A
DA
Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
Wolbers
M
M
Zomerdijk
M
M
Ottens
M
M
Keurentjes
J T F
JT
van der Wielen
L A M
LA
D002850:Chromatography, Gel / Q000379:methods*; D008962:Models, Theoretical; D009211:Myoglobin / Q000302:isolation & purification; D012710:Serum Albumin, Bovine / Q000302:isolation & purification; D013501:Surface-Active Agents / Q000737:chemistry*
D009211:Myoglobin; D013501:Surface-Active Agents; D012710:Serum Albumin, Bovine