Putative odorant-degrading esterase cDNA from the moth Mamestra brassicae: cloning and expression patterns in male and female antennae.
An esterase cDNA was isolated from the cabbage armyworm Mamestra brassicae antennae by PCR strategy. The full-length cDNA, designated as Mbra-EST, contains a 1638 bp open reading frame encoding a predicted protein of 546 amino acids. This predicted protein presents the structural characteristics of known insect carboxyl-esterases, in particular the Ser-His-Glu catalytic triad. The expression pattern of the gene was studied by RT-PCR, Northern-blot and in situ hybridization. The ribosomal protein rpL8 gene from M. brassicae was also cloned to obtain a normalized tool for the comparative gene expression studies. Mbra-EST transcripts are specifically expressed in the antennae of males and females and in the proboscis of males. In antennae of both sexes, expression is restricted to the olfactory sensilla trichodea, suggesting a role in degradation of odorant acetate compounds, such as pheromones as well as plant volatile acetate components.
Maïbèche-Coisne
Martine
M
INRA Unite de Phytopharmacie et Mediateurs Chimiques, Bat. A, route de Saint-Cyr, F-78026 Versailles Cedex, France. maibeche@versailles.inra.fr
Merlin
Christine
C
François
Marie-Christine
MC
Queguiner
Isabelle
I
Porcheron
Patrick
P
Jacquin-Joly
Emmanuelle
E
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000032:analysis; D004950:Esterases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D008297:Male; D008969:Molecular Sequence Data; D009036:Moths / Q000201:enzymology* / Q000378:metabolism; D010675:Pheromones / Q000378:metabolism; D016133:Polymerase Chain Reaction; D018035:Receptors, Odorant / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics
D018076:DNA, Complementary; D010675:Pheromones; D018035:Receptors, Odorant; D004950:Esterases