Interaction of horse heart and thermus thermophilus type c cytochromes with phospholipid vesicles and hydrophobic surfaces.
The binding of horse heart cytochrome c (cyt-c) and Thermus thermophilus cytochrome c(552) (cyt-c(552)) to dioleoyl phosphatidylglycerol (DOPG) vesicles was investigated using Fourier transform infrared (FTIR) spectroscopy and turbidity measurements. FTIR spectra revealed that the tertiary structures of both cytochromes became more open when bound to DOPG vesicles, but this was more pronounced for cyt-c. Their secondary structures were unchanged. Turbidity measurements showed important differences in their behavior bound to the negatively charged DOPG vesicles. Both cytochromes caused the liposomes to aggregate and flocculate, but the ways they did so differed. For cyt-c, more than a monolayer was adsorbed onto the liposome surface prior to aggregation due to charge neutralization, whereas cyt c(552) caused aggregation at a protein/lipid ratio well below that required for charge neutralization. Therefore, although cyt-c may cause liposomes to aggregate by electrostatic interaction, cyt-c(552) does not act in this way. FTIR-attenuated total reflection spectroscopy (FTIR-ATR) revealed that cyt-c lost much of its secondary structure when bound to the hydrophobic surface of octadecyltrichlorosilane, whereas cyt-c(552) folds its domains into a beta-structure. This hydrophobic effect may be the key to the difference between the behaviors of the two cytochromes when bound to DOPG vesicles.
Bernad
Sophie
S
Laboratoire de Dynamique, Interactions et Reactivite, CNRS-Universite Paris VI, Thais, France. sophie.bernad@glvt-cnrs.fr
Oellerich
Silke
S
Soulimane
Tewfik
T
Noinville
Sylvie
S
Baron
Marie-Helène
MH
Paternostre
Maite
M
Lecomte
Sophie
S
D000818:Animals; D003574:Cytochrome c Group / Q000737:chemistry*; D018397:Equidae / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D008081:Liposomes / Q000737:chemistry*; D008958:Models, Molecular; D010715:Phosphatidylglycerols / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D017433:Protein Structure, Secondary; D012821:Silanes / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D016963:Thermus thermophilus / Q000737:chemistry
D003574:Cytochrome c Group; D008081:Liposomes; D010715:Phosphatidylglycerols; D010743:Phospholipids; D012821:Silanes; C061189:octadecyltrichlorosilane; C076615:cytochrome c''; C051388:1,2-dioleoyl-sn-glycero-3-phosphoglycerol