Standardisation of Gymnema sylvestre r. Br. with reference to gymnemagenin by high-performance thin-layer chromatography.
A simple and reproducible HPTLC method for the determination of gymnemagenin (1) in Gymnema sylvestre has been developed. Components were separated on pre-coated silica gel 60 F254 plates with chloroform:methanol (9:1) and scanned using a densitometric scanner in the UV reflectance mode at 290 nm. Linearity of determination of 1 was observed in the range 4-10 microg. The average percentage recovery of 1 from an extract was 99.09 +/- 0.29, and the content of 1 in leaves of the title plant was 1.61% (dry weight).
Puratchimani
V
V
Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra, Ranchi-835 215, India. puratchi_v@rediffmail.com
Jha
S
S
D002855:Chromatography, Thin Layer / Q000379:methods*; D003720:Densitometry; D031104:Gymnema sylvestre / Q000737:chemistry*; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis*
D012503:Saponins