[Effects of baicalin on the expression of pro-MMP-1 and MMP-3 in human gingival fibroblasts and periodontal ligament cells].
OBJECTIVE
To investigate the influence of baicalin on the IL-1beta induced pro-MMP-1 in HGF and the effects of baicalin on MMP-3 expression in periodontal ligament cells (PDLCs).


METHODS
The amount of secreted pro-MMP-1 and MMP-3 expression was detected by ELISA and cell immunochemistry.


RESULTS
(1) The amount of secreted pro-MMP-1 (3.333 +/- 0.123) microg/L increased significantly following 1 microg/L of IL-1beta, compared with control group (1.960 +/- 0.180) microg/L. Addition of baicalin to cell culture medium for 1 hour following IL-1beta decreased pro-MMP-1 secretion in a dose-dependent manner in the range of 10 approximately 1,000 microg/L. (2) 1 microg/L IL-1beta could significantly stimulate the synthesis and secretion of MMP-3 in PDLCs. (3) The baicalin could not interfere the synthesis of MMP-3, but could inhibit the release of MMP-3 from PDLCs.


CONCLUSIONS
Baicalin could inhibit the secretion of pro-MMP-1 and MMP-3 expression in IL-1beta induced HGF and PDLCs, which suggests that baicalin may play an important role in preventing and treating periodontal disease.
Li
Cheng-zhang
CZ
Department of Periodontology, School of Stomatology, Wuhan University, Wuhan 430079, China. lcz56@163.com
Cao
Zheng-guo
ZG
Yang
Ru
R
Shang
Zhu-huan
ZH
Jin
Li-jian
LJ
Cobert
E F
EF
D017364:Collagenases / Q000096:biosynthesis* / Q000235:genetics; D004792:Enzyme Precursors / Q000096:biosynthesis* / Q000235:genetics; D005347:Fibroblasts / Q000201:enzymology / Q000473:pathology; D005419:Flavonoids / Q000494:pharmacology*; D005881:Gingiva / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D053583:Interleukin-1beta; D020781:Matrix Metalloproteinase 1; D008666:Metalloendopeptidases / Q000096:biosynthesis* / Q000235:genetics; D010446:Peptide Fragments / Q000494:pharmacology; D010513:Periodontal Ligament / Q000201:enzymology* / Q000473:pathology; D010518:Periodontitis / Q000201:enzymology / Q000473:pathology; D031363:Scutellaria / Q000737:chemistry
D004792:Enzyme Precursors; D005419:Flavonoids; D007375:Interleukin-1; D053583:Interleukin-1beta; D010446:Peptide Fragments; C052994:interleukin-1beta (163-171); C038044:baicalin; D017364:Collagenases; D008666:Metalloendopeptidases; C048047:prostromelysin; D020781:Matrix Metalloproteinase 1