[Cloning and eukaryotic expression of mouse interleukin 21].
OBJECTIVE
To clone mouse interleukin 21(mIL-21) gene and construct its eukaryotic expression vector.


METHODS
mIL-21 cDNA was amplified from ConA-activated mouse T cells by RT-PCR. And then the cDNA was cloned into eukaryotic expression plasmid pcDNA3.1 to construct recombinant plasmid pcDNA3.1/mIL-21 which was introduced into Sp2/0 cells by lipofectin. Expression of mIL-21 gene was detected by RT-PCR and NK cytotoxicity assay.


RESULTS
Recombinant plasmid pcDNA3.1/mIL-21 was constructed correctly and mIL-21 gene was expressed in transfected Sp2/0 cells.


CONCLUSIONS
mIL-21 gene was cloned and expressed successfully, which lays the foundation for further studying anti-tumor effect of mIL-21 gene therapy in animal models.
Chen
Guo-bing
GB
Department of Microbiology and Immunology, Medical College, Southeast University, Nanjing 210009, China. pawnking@vip.sina.com
Dou
Jun
J
Chen
Jun-song
JS
Hong
Xiao-wu
XW
D000818:Animals; D003001:Cloning, Molecular; D003602:Cytotoxicity, Immunologic; D005260:Female; D015316:Genetic Therapy; D007378:Interleukins / Q000235:genetics*; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000378:metabolism; D014162:Transfection; D000098546:Interleukin-21
D007378:Interleukins; D000098546:Interleukin-21