title: Self-assembling peptide as a potential carrier of hydrophobic compounds.
abstract: Microcrystals of a hydrophobic cargo were stabilized by EAK16 II, a self-assembling oligopeptide, and suspended in aqueous solution. Pyrene was used as a model hydrophobic compound. Egg phosphatidylcholine (EPC) vesicles were prepared to mimic a cell membrane. Pyrene was released from its EAK16 II coating into EPC vesicles. The excimer decay profiles were acquired. They showed that pyrene is present in the crystalline form when stabilized by EAK16 II, it is molecularly dispersed in EPC vesicles, and it is completely released from its EAK16 II coating into the membrane bilayers. The release of pyrene from the microcrystals coated with EAK16 II into the EPC membrane was followed by fluorescence as a function of time. The amount of pyrene released into the EPC vesicles at a given time was quantified using a calibration curve. The concentration of pyrene released was determined as a function of time, and the concentration-versus-time profile was fitted with one exponential. The rate of pyrene release was found to depend on the peptide-to-pyrene molecular ratio. Higher peptide-to-pyrene ratios lead to slower transfer of pyrene to the lipophilic environment. Scanning electron micrographs demonstrated that a thicker coating on the pyrene crystals results in a slower release. The data presented in this work demonstrate that the self-assembling EAK16 II can stabilize a hydrophobic cargo in aqueous solution and deliver it into a lipophilic environment, and that the rate of transfer can be adjusted by tuning the peptide-to-pyrene ratio.
authors.affiliation: Department of Chemistry , University of Waterloo, 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada.
authors.forename: Christine
authors.initials: C
authors.lastname: Keyes
authors.forename: Jean
authors.initials: J
authors.lastname: Duhamel
authors.forename: Shan-Yu
authors.initials: SY
authors.lastname: Fung
authors.forename: Jeremy
authors.initials: J
authors.lastname: Bezaire
authors.forename: P
authors.initials: P
authors.lastname: Chen
mesh_terms: D004337:Drug Carriers / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008051:Lipid Bilayers / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D011721:Pyrenes / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: D004337:Drug Carriers; C098329:EAK16 peptide; D008051:Lipid Bilayers; D008563:Membrane Lipids; D009842:Oligopeptides; D010713:Phosphatidylcholines; D011721:Pyrenes