title: Identification of genes induced in wound-treated wild rice (Oryza minuta).
abstract: A subtracted library was constructed from wound-treated wild rice (Oryza minuta) by suppression subtractive hybridization (SSH) in combination with mirror orientation selection (MOS). To distinguish between differentially expressed transcripts and false positive clones, DNA chips containing 960 random clones were applied as a form of reverse Northern screening. Based on the signal intensities and expression ratios obtained from experiments performed in triplicate, 371 clones were selected. ESTs produced from the subtracted library showed 63.2% redundancy, and 72% of all clones could be matched to the GenBank nonredundant database. Functional categorization placed the identified enriched genes in categories of subcellular localization, metabolism, cell rescue and defense, and transcription. These EST-related resistance mechanisms could be used in investigations into the defense mechanisms of wild species, and to provide new routes to improving the germplasm of cultivated rice.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
authors.forename: Sung Ki
authors.initials: SK
authors.lastname: Cho
authors.forename: Ji Ung
authors.initials: JU
authors.lastname: Jeung
authors.forename: Kyung Ho
authors.initials: KH
authors.lastname: Kang
authors.forename: Kyu Suk
authors.initials: KS
authors.lastname: Shim
authors.forename: Kwang Wook
authors.initials: KW
authors.lastname: Jung
authors.forename: Min Kyoung
authors.initials: MK
authors.lastname: You
authors.forename: Sung Han
authors.initials: SH
authors.lastname: Ok
authors.forename: Young Soo
authors.initials: YS
authors.lastname: Chung
authors.forename: Heung Gu
authors.initials: HG
authors.lastname: Hwang
authors.forename: Hae Chune
authors.initials: HC
authors.lastname: Choi
authors.forename: Huhn Pal
authors.initials: HP
authors.lastname: Moon
authors.forename: Jeong Sheop
authors.initials: JS
authors.lastname: Shin
mesh_terms: D030561:Databases, Nucleic Acid; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*