Induction of MIP-1alpha in Kupffer cell by portal venous transfusion.
BACKGROUND
Previous studies have shown that portal venous transfusion (PVT) induces a state of immunosuppression, and Kupffer cells may be involved in the mechanism.


OBJECTIVE
This study was aimed to investigate the effect of PVT on Kupffer cell gene expression.


METHODS
Each BALB/C mouse was subjected to laparotomy and received one of five treatments: PVT, portal venous saline injection (PVS), inferior vena caval transfusion (IVCT), inferior vena caval saline injection (IVCS) or sham operation (S). The blood for PVT and IVCT was sampled from C57BL/6J mice. Kupffer cells were then isolated 1 or 24 h after each of the 5 treatments, for a total of 10 experimental groups (1-h PVT, PVS, IVCT, IVCS and S, and 24-h PVT, PVS, IVCT, IVCS and S) from BALB/C mice. To examine the effect of PVT on Kupffer cell gene expression, RT-PCR differential display was performed.


RESULTS
Increase in the expression of MIP-1alpha mRNA post PVT and IVCT was identified by differential display. PVT groups revealed higher levels of serum MIP-1alpha than any other groups.


CONCLUSIONS
These results suggest that MIP-1alpha may be involved in a cascade of signaling events associated with the PVT-mediated immunologic modulation in Kupffer cells.
Park
Jong Kwon
JK
Department of Surgery, College of Medicine, Dankook University, Cheonan, Chungnam 330-714, South Korea. hkpark@dankook.ac.kr
Cho
Kiho
K
Johnson
Jeremy
J
Perez
Richard V
RV
D000595:Amino Acid Sequence; D000818:Animals; D001803:Blood Transfusion; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D003001:Cloning, Molecular; D020869:Gene Expression Profiling / Q000379:methods; D007165:Immunosuppression Therapy / Q000379:methods; D007728:Kupffer Cells / Q000276:immunology*; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D011169:Portal Vein; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D017422:Sequence Analysis, DNA; D015854:Up-Regulation
D054405:Chemokine CCL3; D054407:Chemokine CCL4; D019402:Macrophage Inflammatory Proteins; D012333:RNA, Messenger