Phenoxazine compounds produced by the reactions with bovine hemoglobin show antimicrobial activity against non-tuberculosis mycobacteria.
We studied the anti-microbial effects of phenoxazines produced by the reaction of o-aminophenol or its derivatives with bovine hemoglobin, on seven species of mycobacteria such as Mycobacterium tuberculosis, Mycobacterium marinum, Mycobacterium intracellulare, Mycobacterium scrofulaceum, Mycobacterium fortuitum, Mycobacterium kansasii and Mycobacterium smegmatis and some bacteria such as Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica serovar Typhimurium, Staphylococcus aureus, Listeria monocytogeneses. These phenoxazines, including 2-amino-4, 4alpha-dihydro-4alpha, 7-dimethyl-3H-phenoxzine-3-one (Phx-1), 3-amino-1, 4alpha-dihydro-4alpha, 8-dimethyl-2H-phenoxazine-2-one (Phx-2), and 2-aminophenoxazine-3-one (Phx-3), prevented the proliferation of four non-tuberculosis mycobacteria including M. scrofulaceum, M. kansasii, M. marinum, and M. intracellulare dose-dependently, though the inhibitory effects of these phenoxazines differed according to the species of mycobacteria. However these phenoxazines failed to prevent the proliferation of M. tuberculosis, M. fortuitum, and M. smegmatis, and the concerned bacteria other than mycobacteria. The present results may contribute to development of novel antibiotics against non-tuberculolsis mycobacteria.
Shimizu
Shigetaka
S
Department of Otorhinolaryngology, Research Institute of Immunological Treatment, Tokyo Medical University, Tokyo 160-0032, Japan.
Suzuki
Mamoru
M
Tomoda
Akio
A
Arai
Sadao
S
Taguchi
Haruhiko
H
Hanawa
Tomoko
T
Kamiya
Shigeru
S
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D002417:Cattle; D006454:Hemoglobins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D009161:Mycobacterium / Q000187:drug effects* / Q000254:growth & development; D010078:Oxazines / Q000737:chemistry* / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D006454:Hemoglobins; D010078:Oxazines; C039203:phenoxazine