In vivo and in vitro anti-inflammatory activity of Mangifera indica L. extract (VIMANG).
A standard aqueous extract of Mangifera indica L., used in Cuba as an antioxidant under the brand name of VIMANG, was tested in vivo for its anti-inflammatory activity using commonly accepted assays. M. indica extract, administered topically (0.5-2 mg per ear), reduced ear edema induced by arachidonic acid (AA) and phorbol myristate acetate (PMA, ED50 = 1.1 mg per ear) in mice. In the PMA model, M. indica extract also reduced myeloperoxidase (MPO) activity. This extract p.o. administered also inhibited tumor necrosis factor alpha (TNFalpha) serum levels in both models of inflammation (AA, ED50 = 106.1 mg kg(-1) and PMA, ED50 = 58.2 mg kg(-1)). In vitro studies were performed using the macrophage cell line RAW264.7 stimulated with pro-inflammatory stimuli (LPS-IFNgamma or the calcium ionophore A23187) to determine PGE2 or LTB4 release, respectively. The extract inhibited the induction of PGE2 with IC50 = 64.1 microg ml(-1) and LTB4 IC50 = 22.9 microg ml(-1). M. indica extract also inhibited human synovial secretory phospholipase (PL)A2 with IC 50 = 0.7 microg ml(-1). These results represent an important contribution to the elucidation of the mechanism involved in the anti-inflammatory and anti-nociceptive effects reported by the standard M. indica extract VIMANG.
Garrido
Gabino
G
Laboratorio de Farmacología. Centro de Química Farmacéutica, Atabey, 200 y 21 Atabey, Playa, Apartado Postal 16042 Ciudad de La Habana, Cuba. gabino.garrido@infomed.sld.cu
González
Deyarina
D
Lemus
Yeny
Y
García
Dagmar
D
Lodeiro
Lizt
L
Quintero
Gypsy
G
Delporte
Carla
C
Núñez-Sellés
Alberto J
AJ
Delgado
René
R
D000287:Administration, Topical; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D016718:Arachidonic Acid / Q000008:administration & dosage / Q000009:adverse effects / Q000037:antagonists & inhibitors; D000001:Calcimycin / Q000494:pharmacology; D003462:Cuba; D003907:Dexamethasone / Q000494:pharmacology; D015232:Dinoprostone / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004359:Drug Therapy, Combination; D004431:Ear, External / Q000187:drug effects / Q000503:physiopathology; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D015777:Eicosanoids / Q000378:metabolism; D007213:Indomethacin / Q000494:pharmacology; D007371:Interferon-gamma / Q000378:metabolism / Q000494:pharmacology; D007975:Leukotriene B4 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D031022:Mangifera / Q000737:chemistry*; D051379:Mice; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D009195:Peroxidase / Q000009:adverse effects / Q000037:antagonists & inhibitors; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000378:metabolism; D008517:Phytotherapy; D024301:Plant Bark; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D018547:Plant Stems; D010946:Plants, Medicinal / Q000737:chemistry; D013755:Tetradecanoylphorbol Acetate / Q000008:administration & dosage / Q000009:adverse effects / Q000037:antagonists & inhibitors; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000378:metabolism; D014867:Water; D044004:Xanthones / Q000494:pharmacology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D015777:Eicosanoids; D008070:Lipopolysaccharides; C501277:Mangifera indica extract; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D044004:Xanthones; C118309:petrosaspongiolide m; D014867:Water; D007975:Leukotriene B4; C013592:mangiferin; D016718:Arachidonic Acid; D000001:Calcimycin; D009828:Oleanolic Acid; D003907:Dexamethasone; D007371:Interferon-gamma; D009195:Peroxidase; D010741:Phospholipases A; D015232:Dinoprostone; D013755:Tetradecanoylphorbol Acetate; D007213:Indomethacin