[The effect of somatostatin analogue on expression of connective tissue growth factor gene of rat hepatic stellate cells].
OBJECTIVE
To investigate the effect of somatostatin analogue-octreotide (OCT) on expression of connective tissue growth factor (CTGF) gene of murine hepatic stellate cells (HSCs) in vitro.


METHODS
HSCs separated from Sprague Dawley rats by in situ perfusion and Nycodenz gradient were divided into 5 groups. HSCs in 4 out of 5 groups were co-cultured with octreotide at different dosages, and the remaining group served as control. The expression of CTGF and TGF-beta mRNA were assessed by reverse transcription-polymerase chain reaction (RT-PCR).


RESULTS
OCT down-regulates the expression of CTGF and TGF-beta mRNA in HSCs. The effect is increased with a dose dependent manner.


CONCLUSIONS
OCT could exert the inhibitory effect on HSCs by down-regulating the expression of CTGF and TGF-beta. This provides a potential for the prevention and management of hepatic fibrosis.
Song
Sheng-han
SH
Department of Hepatobiliary Surgery, Peking University People's Hospital, Beijing 100044, China.
Leng
Xi-sheng
XS
Li
Tao
T
Peng
Ji-run
JR
Zhao
Li
L
Qin
Zhi-zhong
ZZ
Wei
Yu-hua
YH
Yu
Xin
X
D000818:Animals; D002478:Cells, Cultured; D055513:Connective Tissue Growth Factor; D015870:Gene Expression / Q000187:drug effects; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D017874:Immediate-Early Proteins / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D008297:Male; D015282:Octreotide / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013004:Somatostatin / Q000031:analogs & derivatives; D016212:Transforming Growth Factor beta / Q000235:genetics*
C526316:CCN2 protein, rat; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D055513:Connective Tissue Growth Factor; D013004:Somatostatin; D015282:Octreotide