[Activation of mouse macrophages by the alkali-extracted polysaccharide from spore of Ganoderma lucidum].
OBJECTIVE
To investigate the activation of mouse macrophages by the alkali-extracted polysaccharides from the spore of Ganoderma lucidum (LZSBS).


METHODS
The mouse macrophages cultured in-vitro were stimulated by LZSBS. IL-1beta and TNF-alpha in the culture supernatants were detected by ELISA. NO production was detected by Griess assay. The percentage of phagocytosis of latex beads by mouse macrophages was counted under microscope.


RESULTS
The mouse macrophages stimulated by LZSBS increased in volume and darkened in appearance under phase-contrast microscope. LZSBS-activated mouse macrophages secreted IL-1beta and TNF-alpha produced a large amount of NO. The percentage of phagocytosis of latex beads by mouse macrophages was also significantly increased in the presence of LZSBS.


CONCLUSIONS
LZSBS can activate markedly the mouse macrophages.
Tang
Qing-jiu
QJ
Institute of Molecularbiology and Biochemistry, Free University Berlin, Berlin, Germany. tangqingjiu2003@yahoo.com.cn
Zhang
Jin-song
JS
Pan
Ying-jie
YJ
Reutter
Werner
W
Fan
Hua
H
D000818:Animals; D007375:Interleukin-1 / Q000378:metabolism*; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D020940:Reishi / Q000737:chemistry; D013172:Spores, Fungal / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D007375:Interleukin-1; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide