[Effects of c-jun on hCG-induced testosterone secretion of rat Leydig cells in vitro].
OBJECTIVE
To investigate the effects of c-jun on hCG-induced testosterone secretion in isolated rat Leydig cells by antisense oligodeoxynucleotides(ASODNs).


METHODS
c-jun ASODNs were used to antagonise the effects of c-jun, hCG was used to induce the testosterone secretion of LC cultured in vitro and testosterone was measured by radioimmunoassay.


RESULTS
The testosterone secretion of LC in vitro could be induced by hCG, which was a good model for the functional study of LC. c-jun ASODNs decreased the hCG-induced testosterone secretion of LC in a dose-dependent manner(P < 0.05).


CONCLUSIONS
It is suggested that c-jun proto-oncogene enhances the testosterone secretion of LC.
Yuan
Shuanghu
S
Department of Radiology, Shandong Tumor Hospital & Institute, Jinan, Shandong 250117, China. yuanshuanghu@sina.com
Xu
Sifan
S
Yang
Xinhua
X
Liu
Xiuqing
X
Hao
Junfang
J
Qian
Ming
M
D000818:Animals; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D016755:Proto-Oncogene Proteins c-jun / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D016376:Oligonucleotides, Antisense; D016755:Proto-Oncogene Proteins c-jun; D013739:Testosterone