title: Molecular signature linked to acquired resistance to cisplatin in esophageal cancer cells.
abstract: To clarify the molecular basis of acquired cisplatin (CDDP) resistance in esophageal squamous cell carcinoma (ESCC), we used cDNA microarray technology. A CDDP-resistant cell line (YES-2/CDDP), which shows a 7.5-fold increase in resistance to CDDP and a 3-fold decrease in CDDP accumulation compared with the parental YES-2 ESCC cell line, was generated from YES-2 by exposure to increased concentrations of CDDP. By cDNA microarray analysis, we identified 44 genes with significantly different expression levels between YES-2/CDDP and YES-2 cells. Interestingly, 15 of these 44 genes encoded ribosome-related proteins, almost all of which were underexpressed in YES-2/CDDP cells. Our present data suggest that many ribosome-related genes may be involved in the acquired resistance to CDDP in ESCC and that such information may allow us to better understand the mechanism of CDDP resistance.
authors.affiliation: Department of Pathology II, Yamaguchi University School of Medicine, 1-1-1 Minami-kogushi, Ube, Yamaguchi 755-8505, Japan. h.10432@po.cc.yamaguchi-u.ac.jp
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Toshimitsu
authors.forename: Kiichiro
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Akira
authors.initials: A
authors.lastname: Tangoku
authors.forename: Norio
authors.initials: N
authors.lastname: Iizuka
authors.forename: Kohtaro
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Shigeto
authors.initials: S
authors.lastname: Kawauchi
authors.forename: Atsunori
authors.initials: A
authors.lastname: Oga
authors.forename: Tomoko
authors.initials: T
authors.lastname: Furuya
authors.forename: Masaaki
authors.initials: M
authors.lastname: Oka
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Sasaki
mesh_terms: D000970:Antineoplastic Agents; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology / Q000627:therapeutic use*; D053938:DNA Fragmentation / Q000187:drug effects; D019008:Drug Resistance, Neoplasm; D004938:Esophageal Neoplasms / Q000188:drug therapy*; D018628:Gene Dosage; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000970:Antineoplastic Agents; D002945:Cisplatin