Molecular cloning of superoxide dismutase (Cu/Zn-SOD) from aquatic molluscs.
The potential of the first line of the active oxygen-scavenging system, partial cDNA encoding Cu/Zn superoxide dismutase (SOD) was isolated in three aquatic mollusc species: Ruditapes decussatus (marine clam), Dreissena polymorpha (continental water mussel) and Bathymodiolus azoricus (hydrothermal vent mussel). These SOD cDNA fragments were amplified by PCR with degenerate oligonucleotide primers derived from the amino acid sequence conserved in the Cu/Zn-SOD from several other organisms. A partial cDNA of CuZn-SOD was obtained for R. decussates (510 bp), D. polymorpha (510 bp) and B. azoricus (195 bp). The deduced amino acid sequence showed high similarity among the three mollusc species (57-63%) and among other species (50-65%). The residues involved in coordinating copper (His-47, 49, 64, 121) and zinc (His-64, 72, 81 and Asp-84) were well conserved among the three Cu/Zn-SOD sequences.
Geret
F
F
DEL-PC, Ifremer, Rue de l'île d'Yeu, B.P. 21105, 44311 Nantes Cedex 3, France.
Manduzio
H
H
Company
R
R
Leboulenger
F
F
Bebianno
M J
MJ
Danger
J M
JM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000235:genetics* / Q000378:metabolism; D017124:Conserved Sequence; D003300:Copper / Q000633:toxicity; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004790:Enzyme Induction / Q000187:drug effects; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000235:genetics*; D013997:Time Factors
D017931:DNA Primers; D018076:DNA, Complementary; D003300:Copper; D013482:Superoxide Dismutase