The mannose receptor fails to enhance processing and presentation of a glycoprotein antigen in transfected fibroblasts.
One function proposed for the mannose receptor found on dendritic cells as well as on macrophages and hepatic endothelial cells is in enhancing uptake and processing of glycoprotein antigens for presentation by major histocompatibility complex (MHC) class II molecules. In this study, a direct assessment of the possible role of the mannose receptor in this process was made in the absence of other endocytic receptors that can internalize glycoproteins. Presentation of RNase A and B peptides was compared in transfected fibroblasts coexpressing the mannose receptor and MHC class II molecules. RNase B bears a high-mannose oligosaccharide and is a ligand for the mannose receptor, whereas RNase A is not glycosylated and is taken up by pinocytosis. Incubation of RNase A or B with the transfected cells resulted in identical stimulation of ribonuclease-specific T cells, indicating that endocytosis of the glycosylated protein by the mannose receptor does not enhance presentation of this antigen. The postulated role of the mannose receptor in presentation of glycoprotein-derived antigen is reevaluated in light of these results.
Napper
Catherine E
CE
Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
Taylor
Maureen E
ME
D000818:Animals; D017951:Antigen Presentation / Q000235:genetics / Q000502:physiology*; D000941:Antigens / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line; D005347:Fibroblasts / Q000276:immunology / Q000378:metabolism*; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000502:physiology; D006801:Humans; D037181:Lectins, C-Type / Q000235:genetics / Q000502:physiology*; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000235:genetics / Q000502:physiology*; D012259:Ribonuclease, Pancreatic / Q000276:immunology / Q000378:metabolism; D012260:Ribonucleases / Q000276:immunology / Q000378:metabolism; D014162:Transfection
D000941:Antigens; D006023:Glycoproteins; D000949:Histocompatibility Antigens Class II; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; D012260:Ribonucleases; C029600:ribonuclease B; D012259:Ribonuclease, Pancreatic