title: [Construction and characterization of osteosarcoma 9901 cell cDNA library].
abstract: OBJECTIVE
  To construct human osteosarcoma 9901 cell cDNA expression library for screening osteosarcoma-specific antigens.
  
  
  METHODS
  Total RNA was extracted from human osteosarcoma cell line 9901 and mRNA was purified. cDNA was synthesized by reverse transcription, and cDNA fragments larger than 400 bp were ligated with dephosphorylated arms of lambdagt11. The recombinants were packaged in-vitro, and a small portion of packaged phage was used to infect E.coli Y1090 for titration. The size of cDNA inserts and the diversity of library were detected by PCR.
  
  
  RESULTS
  The osteosarcoma 9901 cell line cDNA library consisting of 1.5x10(6) recombinant bacteriophages was constructed. The average length of exogenous inserts in the recombinants was about 1.4 kb.
  
  
  CONCLUSIONS
  The cDNA library reported herein is suitable for screening osteosarcoma-specific antigens.
authors.affiliation: Department of Orthopedics, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. liaobo_@163.com
authors.forename: Bo
authors.initials: B
authors.lastname: Liao
authors.forename: Bao-an
authors.initials: BA
authors.lastname: Ma
authors.forename: Hui-zhong
authors.initials: HZ
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Long
authors.forename: De-gang
authors.initials: DG
authors.lastname: Fan
authors.forename: Qing-yu
authors.initials: QY
authors.lastname: Fan
mesh_terms: D001859:Bone Neoplasms / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D012516:Osteosarcoma / Q000235:genetics*; D016133:Polymerase Chain Reaction