[Content determination of S-citalopram by chiral high-performance liquid chromatography].
OBJECTIVE
To separate the enantiomers of citalopram and determine the content of S-citalopram using chiral high-performance liquid chromatography (HPLC).


METHODS
The chiral column CHIROBIOTIC V was used with the mobile phase using methanol-acetic acid-triethylamine (100:0.1:0.1) at the detection wavelength of 240 nm, column temperature of 20 degrees Celsius and flow rate of 1.0 ml/min.


RESULTS
Complete separation of the enantiomers of citalopram was achieved, and S-citalopram exhibited good linearity within the concentration range of 10 to 150 microg/ml (r=0.9991, n=5).


CONCLUSIONS
This method allows accurate quantification of S- and R-citalopram and is well suited for drug interaction investigations.
Yang
Xue-mei
XM
Department of Chemistry, First Military Medical University, Guangzhou 510515, China.
Liu
Xu
X
Yan
Yi-chen
YC
Xu
Jiang-ping
JP
D002851:Chromatography, High Pressure Liquid; D015283:Citalopram / Q000032:analysis*; D013237:Stereoisomerism
D015283:Citalopram