title: RalA but not RalB enhances polarized delivery of membrane proteins to the basolateral surface of epithelial cells.
abstract: RalA and RalB constitute a family of highly similar (85% identity) Ras-related GTPases. Recently, active forms of both RalA and RalB have been shown to bind to the exocyst complex, implicating them in the regulation of cellular secretion. However, we show here that only active RalA enhances the rate of delivery of E-cadherin and other proteins to their site in the basolateral membrane of MDCK cells, consistent with RalA being a regulator of exocyst function. One reason for this difference is that RalA binds more effectively to the exocyst complex than active RalB does both in vivo and in vitro. Another reason is that active RalA localizes to perinuclear recycling endosomes, where regulation of vesicle sorting is thought to take place, while active RalB does not. Strikingly, analysis of chimeras made between RalA and RalB reveals that high-affinity exocyst binding by RalA is due to unique amino acid sequences in RalA that are distal to the common effector-binding domains shared by RalA and RalB. Moreover, these chimeras show that the perinuclear localization of active RalA is due in part to its unique variable domain near the C terminus. This distinct localization appears to be important for RalA effects on secretion because all RalA mutants tested that failed to localize to the perinuclear region also failed to promote basolateral delivery of E-cadherin. Interestingly, one of these inactive mutants maintained binding to the exocyst complex, suggesting that RalA binding to the exocyst is necessary but not sufficient for RalA to promote basolateral delivery of membrane proteins.
authors.affiliation: Department of Biochemistry, Tufts University School of Medicine, Boston, MA 02111, USA.
authors.forename: Michail
authors.initials: M
authors.lastname: Shipitsin
authors.forename: Larry A
authors.initials: LA
authors.lastname: Feig
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D016764:Cell Polarity / Q000502:physiology*; D004285:Dogs; D011992:Endosomes / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism*; D020558:GTP Phosphohydrolases / Q000502:physiology*; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000378:metabolism*; D011485:Protein Binding; D021381:Protein Transport; D011993:Recombinant Fusion Proteins; D020725:ral GTP-Binding Proteins / Q000502:physiology*
chemical_list: D015820:Cadherins; D046911:Macromolecular Substances; D008565:Membrane Proteins; C082190:Ralb protein, rat; D011993:Recombinant Fusion Proteins; D020558:GTP Phosphohydrolases; C492197:Rala protein, rat; D020725:ral GTP-Binding Proteins