[Effect of homocysteine on NF-kappaB activity in cultured rat vascular smooth muscle cells].
To investigate the effect of homocysteine (Hcy) on nuclear factor-kappaB (NF-kappaB) activity in cultured rat vascular smooth muscle cells (VSMCs).



Rat VSMCs were stimulated with 0.25 mmol/L Hcy. Cells were collected and nuclear protein was extracted at 30 min, 1 h and 2 h following stimulation. NF-kappaB activity was examined by electrophoretic mobility shift assay (EMSA).



Baseline NF-kappaB nuclear binding in VSMCs increased more than 1.76-fold at 30 min (P<0.01), 1.91-fold at 1h (P<0.01) and 1.84-fold at 2 h (P<0.01) following stimulation of Hcy.



Hcy can enhance transient mobilization of N-kappaB in VSMCs, which suggests that some regulating effect of Hcy on VSMCs might be exerted through NF-kappaB activation pathway.
Zheng
Liang-rong
LR
The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Zhu
Jian-hua
JH
Zhang
Li
L
D000818:Animals; D001161:Arteriosclerosis / Q000209:etiology; D024202:Electrophoretic Mobility Shift Assay; D005260:Female; D006710:Homocysteine / Q000494:pharmacology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D016328:NF-kappa B; D006710:Homocysteine