Characterisation of the core part of the lipopolysaccharide O-antigen of Francisella novicida (U112).
Francisella novicida (U112), a close relative of the highly virulent bacterium F. tularensis, is known to produce a lipopolysaccharide that is significantly different in biological properties from the LPS of F. tularensis. Here we present the results of the structural analysis of the F. novicida LPS core part, which is found to be similar to that of F. tularensis, differing only by one additional alpha-Glc residue:where R is an O-chain, linked via a beta-bacillosamine (2,4-diamino-2,4,6-trideoxyglucose) residue. The lipid part of F. novicida LPS contains no phosphate substituent and apparently has a free reducing end, a feature also noted in F. tularensis LPS.
Vinogradov
Evgeny
E
Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada K1A 0R6. evguenii.vinogradov@nrc-cnrc.gc.ca
Perry
Malcolm B
MB
D019342:Acetic Acid / Q000737:chemistry; D000096:Acetone / Q000737:chemistry; D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005603:Francisella / Q000276:immunology* / Q000472:pathogenicity; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology; D012015:Reference Standards; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002247:Carbon Isotopes; D008070:Lipopolysaccharides; D019081:O Antigens; D000096:Acetone; D019342:Acetic Acid