title: Upregulation of GluR2 decreases intracellular Ca2+ following ischemia in developing gerbils.
abstract: Developing animals are known to be resistant to cerebral ischemia. To investigate the mechanisms by which developing animals exhibit ischemic resistance, we examined the changes in intracellular calcium ([Ca2+]i) after oxygen-glucose deprivation (OGD) using hippocampal slices from gerbils. We found that increases of [Ca2+]i in hippocampal CA1 neurons is significantly less after OGD in developing gerbils than in adults. Western blot analysis of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid) receptors (AMPARs) showed that GluR2 expression, but not that of the other AMPARs is significantly higher in developing gerbils than in adults. Expression of the anti-apoptotic proteins such as HSP70, Bcl-XL, and plasma membrane Ca2+-ATPase type1 (PMCA1) are not higher in the developing gerbils than in adults. These results suggest that the higher expression of GluR2 is important for the smaller increases in [Ca2+]i and enhanced resistance to ischemia-induced neuronal damage in developing animals.
authors.affiliation: Department of Surgical Neurology, Jichi Medical School, Minamikawachi-machi, Tochigi 329-0498, Japan. oguro@jichi.ac.jp
authors.forename: Keiji
authors.initials: K
authors.lastname: Oguro
authors.forename: Takahiro
authors.initials: T
authors.lastname: Miyawaki
authors.forename: Hidenori
authors.initials: H
authors.lastname: Yokota
authors.forename: Kengo
authors.initials: K
authors.lastname: Kato
authors.forename: Tatsushi
authors.initials: T
authors.lastname: Kamiya
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Katayama
authors.forename: Masahiro
authors.initials: M
authors.lastname: Fukaya
authors.forename: Masahiko
authors.initials: M
authors.lastname: Watanabe
authors.forename: Kuniko
authors.initials: K
authors.lastname: Shimazaki
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D015153:Blotting, Western; D002545:Brain Ischemia / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000096:biosynthesis / Q000235:genetics; D017536:Carotid Artery, Common / Q000502:physiology; D005456:Fluorescent Dyes; D005849:Gerbillinae; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D006624:Hippocampus / Q000378:metabolism; D066298:In Vitro Techniques; D018091:Receptors, AMPA / Q000096:biosynthesis* / Q000235:genetics*; D015854:Up-Regulation / Q000502:physiology*
chemical_list: D005456:Fluorescent Dyes; D018840:HSP70 Heat-Shock Proteins; D018091:Receptors, AMPA; D000252:Calcium-Transporting ATPases; C104722:glutamate receptor ionotropic, AMPA 2; D002118:Calcium