title: [Hematopoietic stem/progenitor cells and phenotypes of lymphocyte subpopulations in human placenta].
abstract: OBJECTIVE
  To study whether human placenta contains hematopoietic stem/progenitor cells (HSPCs), and analyze phenotypes of lymphocyte subpopulations in the placenta.
  
  
  METHODS
  Nucleated cells from fresh human placenta were analyzed for phenotypes of HSPCs and lymphocyte subpopulations by flow cytometry (FCM). And CD(34)(+) cells were sorted from human placenta nucleated cells by FCM or MiniMACS.
  
  
  RESULTS
  (1) CD(34)(+) cells, CD(34)(+)/CD(38)(+) cells, and CD(34)(+)/CD(38)(-) cells from a human placenta were 8.8, 4.6 and 11.9 times higher than those from umbilical cord blood (UCB), respectively. (2) The yields and purity of CD(34)(+) cells isolated from human placenta by FCM sorting system were (63.05 +/- 10.14)% and (86.39 +/- 11.27)%, respectively. (3) Lymphocytes, T cells (CD(3)(+)/CD(2)(+)), B cells (CD(19)(+)), Th cells (CD(3)(+), CD(4)(+)), and Th/Ts ratio in the placenta tissue were apparently lower than those in the UCB, while the CD(8)(+)/CD(28)(-) T suppressor cells were higher in the placenta than in the UCB.
  
  
  CONCLUSIONS
  Human placenta is rich in HSPCs, and has important hematopoietic function in ontogeny. It is probable that human placenta would be graft resource for HSPCs transplantation. CD(8)(+)/CD(28)(-) T suppressor cells might play an important role in feto-maternal immunologic tolerance.
authors.affiliation: The Affiliated Hospital of Zunyi Medical College, Key Laboratory of Cell Engineering of Guizhou Province, Zunyi 563003, China.
authors.forename: Dai-Xiong
authors.initials: DX
authors.lastname: Chen
authors.forename: Ning
authors.initials: N
authors.lastname: Fang
authors.forename: Zu-Lin
authors.initials: ZL
authors.lastname: Liu
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Wan
authors.forename: Ying
authors.initials: Y
authors.lastname: Qi
authors.forename: Jin-Wei
authors.initials: JW
authors.lastname: Liu
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Xiao
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
mesh_terms: D002478:Cells, Cultured; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology*; D006801:Humans; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000166:cytology / Q000276:immunology*; D008297:Male; D010920:Placenta / Q000166:cytology / Q000276:immunology*; D011247:Pregnancy