title: [Preparation and identification of the monoclonal antibody against human endostatin].
abstract: OBJECTIVE
  To prepare monoclonal antibody against human endostatin for the in depth study on the mechanism of anti-tumor effect of endostatin.
  
  
  METHODS
  Monoclonal antibody specific for endostatin was prepared using hybridoma technique and screened with ELISA. Ascites fluids were produced in BALB/c mice following in sequentical intraperitoneal injection of pristine and hybridoma cells. The mAb was purified by affinify chromatography with protein A sepharose CL-4B.
  
  
  RESULTS
  One hybridoma cell line 4E7 was established, which could produce mAb against human endostatin. The titers of mAb 4E7 in culture supernatant and ascites fluid were 1:128-1:516 and 1:10(-4)-1:10(-6), respectively. The mAb 4E7 belonged to IgG1 (lambda type). Western blot analysis showed that the mAb 4E7 could react to the human endostatin expressed by yeast and E.coli, but it had no cross reaction to other cytokines such as bFGF.
  
  
  CONCLUSIONS
  The mAb 4E7 is able to react specifically with human endostatin and can be used for further investigation.
authors.affiliation: Department of Genetic Engineering, Beijing Institute of Biotechnology, Beijing 100850, China. feifeicat@mailcity.com
authors.forename: Yu-mei
authors.initials: YM
authors.lastname: Feng
authors.forename: Yi
authors.initials: Y
authors.lastname: Feng
authors.forename: Yan-hong
authors.initials: YH
authors.lastname: Zhang
authors.forename: Lie
authors.initials: L
authors.lastname: Ren
authors.forename: Zhao-chun
authors.initials: ZC
authors.lastname: Zeng
authors.forename: Qing-jun
authors.initials: QJ
authors.lastname: Ma
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D043169:Endostatins / Q000276:immunology*; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D043169:Endostatins