Voltage-gated sodium channel expressed in cultured human smooth muscle cells: involvement of SCN9A.
Voltage-gated Na(+) channel (I(Na)) is expressed under culture conditions in human smooth muscle cells (hSMCs) such as coronary myocytes. The aim of this study is to clarify the physiological, pharmacological and molecular characteristics of I(Na) expressed in cultured hSMCs obtained from bronchus, main pulmonary and coronary artery. I(Na), was recorded in these hSMCs and inhibited by tetrodotoxin (TTX) with an IC(50) value of approximately 10 nM. Reverse transcriptase/polymerase chain reaction (RT-PCR) analysis of mRNA showed the prominent expression of transcripts for SCN9A, which was consistent with the results of real-time quantitative RT-PCR. These results provide novel evidence that TTX-sensitive Na(+) channel expressed in cultured hSMCs is mainly composed of Na(v)1.7.
Jo
Taisuke
T
Department of Cardiovascular, Respiratory Medicine and Gastroenterology, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
Nagata
Taiji
T
Iida
Haruko
H
Imuta
Hiroyuki
H
Iwasawa
Kuniaki
K
Ma
Ji
J
Hara
Kei
K
Omata
Masao
M
Nagai
Ryozo
R
Takizawa
Hajime
H
Nagase
Takahide
T
Nakajima
Toshiaki
T
D001980:Bronchi / Q000166:cytology; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000166:cytology; D004594:Electrophysiology; D015870:Gene Expression; D006801:Humans; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D062556:NAV1.7 Voltage-Gated Sodium Channel; D009543:Nifedipine / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D011651:Pulmonary Artery / Q000166:cytology; D026941:Sodium Channel Blockers / Q000494:pharmacology; D015222:Sodium Channels / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D013779:Tetrodotoxin / Q000494:pharmacology; D014018:Tissue Distribution
D062556:NAV1.7 Voltage-Gated Sodium Channel; C486866:SCN9A protein, human; D026941:Sodium Channel Blockers; D015222:Sodium Channels; D013779:Tetrodotoxin; D009543:Nifedipine