title: [Participation of ICAM-1 in the cytotoxicity of TM-TNF-alpha mediated by TNF receptor I].
abstract: OBJECTIVE
  To identify membrane-associated molecules involved in the tumoricidal cytotoxicity of TM-TNF-alpha and to explore the molecular mechanism underlying the tumoricidal cytotoxicity of TM-TNF-alpha and differences of biological effects between TM-TNF-alpha and sTNF.
  
  
  METHODS
  Antibody blocking test and RT-PCR were used to evaluate the role of ICAM-1 and VCAM-1 in the cytotoxicity of TM-TNF-alpha mediated by TNFR I or TNFR II.
  
  
  RESULTS
  After the ICAM-1 expressed on the membrane of MCF-7 cells(expressing TNFRI) was blocked, the cytotoxicity of TM-TNF-alpha to this cell lines decreased significantly, while blocking VCAM-1 had no effect. Blocking ICAM-1 or VCAM-1 expressed on the membrane of HL-60 cells(expressing TNFRII)had no significant effects on the cytotoxicity of TM-TNF-alpha to this cell lines.
  
  
  CONCLUSIONS
  ICAM-1 participates in the cytotoxicity of TM-TNF mediated by TNFRI.
authors.affiliation: Institute of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. zhengfangtj@yahoo.com
authors.forename: Fang
authors.initials: F
authors.lastname: Zheng
authors.forename: Wen-fang
authors.initials: WF
authors.lastname: Shi
authors.forename: Wei
authors.initials: W
authors.lastname: Feng
authors.forename: Xiao-dan
authors.initials: XD
authors.lastname: Jiang
authors.forename: Yong
authors.initials: Y
authors.lastname: Xu
authors.forename: Fang
authors.initials: F
authors.lastname: Fan
authors.forename: Zhuo-ya
authors.initials: ZY
authors.lastname: Li
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology*; D051379:Mice; D018124:Receptors, Tumor Necrosis Factor / Q000502:physiology*; D047888:Receptors, Tumor Necrosis Factor, Type I; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000502:physiology
chemical_list: D015703:Antigens, CD; D018124:Receptors, Tumor Necrosis Factor; D047888:Receptors, Tumor Necrosis Factor, Type I; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1