Polymorphism in the N-acetyltransferase 1 alleles NAT1*10 and NAT1*14A and cytological gradings of exfoliated urothelial cells in benzidine-exposed Chinese workers: discussion of ethnic differences.
N-acetyltransferase 1 (NAT1) modifies bladder cancer risk in European populations exposed to aromatic amines in cigarette smoke. The present study was performed to investigate a possible association between NAT1*10 and NAT1*14A genotypes and bladder cancer risk in benzidine-exposed Chinese workers. Based on the cytological gradings of exfoliated urothelial cells according to Papanicolaou, an exposed research cohort was stratified into subgroups. An allele-specific PCR-based procedure was used to detect the polymorphism in the polyadenylation signal at the locus NAT1 T(1088)A. A nested PCR-RFLP procedure was conducted to differentiate NAT1*14A (T(1088)A, C(1095)A, and G(560)A) from NAT1*10 (T(1088)A, C(1095)A). No significantly different frequencies of homozygous and heterozygous NAT1*10 alleles were found among the subgroups with (i) gradings according to Papanicolaou < or = II (18.3 and 40.2%, respectively), (ii) higher gradings according to Papanicolaou (> II; 28.0 and 34.1%, respectively), and (iii) with bladder cancer (26.3 and 34.2%, respectively). The present data show that NAT1*10 neither displayed an association with an elevated grading of urothelial cells nor a clear impact on the risk for bladder cancer in benzidine-exposed Chinese workers. Discrepancies with the findings in European populations could point to ethnic differences in the disposition of aromatic amines.
Guo
Wei Chao
WC
Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 225 Chongqing Rd. (S.), 200025 Shanghai, China.
Lin
Guo Fang
GF
Chen
Ji Gang
JG
Golka
Klaus
K
Shen
Jian Hua
JH
D000328:Adult; D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D001560:Benzidines / Q000009:adverse effects*; D002273:Carcinogens / Q000009:adverse effects*; D002295:Carcinoma, Transitional Cell / Q000139:chemically induced / Q000453:epidemiology / Q000235:genetics; D002681:China / Q000453:epidemiology; D004273:DNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D005006:Ethnicity; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008297:Male; D016273:Occupational Exposure; D065006:Papanicolaou Test; D011110:Polymorphism, Genetic / Q000235:genetics*; D012907:Smoking / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced / Q000453:epidemiology / Q000235:genetics; D019459:Urothelium / Q000166:cytology*; D014626:Vaginal Smears
D001560:Benzidines; D002273:Carcinogens; D004273:DNA, Neoplasm; D007527:Isoenzymes; D001191:Arylamine N-Acetyltransferase; C089384:N-acetyltransferase 1