Ginsenoside Rg1 promotes proliferation of hippocampal progenitor cells.
Neural progenitor cells (NPCs) exist not only in the developing brain, but also in certain areas in adult brain in mammals. Recent studies suggest that promoting neurogenesis in adult mammals might provide a therapeutic way to cure age-related neurodegenerative diseases. So, it will be of great value to find out drugs that can increase the proliferation and/or differentiation ability of neural progenitors. The present study investigated the influence of ginsenoside Rg1, an active ingredient of Panax ginseng C.A. Meyer, on proliferation ability of rodent hippocampal progenitor cells both in vitro and in vivo. Incubation of NPCs with ginsenoside Rg1 resulted in significant increase in absorbency value, 3H-thymidine incorporation and the number of proliferating progenitor cell spheres; In addition, 2 weeks Rg1 administration (i.p.) led to marked enhancement of the number of dividing cells in the hippocampus of adult mice. These findings suggest that ginsenoside Rg1 is involved in the regulation of proliferation of hippocampal progenitor cells and this effect may serve as one of the elementary mechanisms underlying its nootropic and anti-aging actions.
Shen
Li-Hong
LH
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, PR of China.
Zhang
Jun-Tian
JT
D000818:Animals; D000831:Animals, Newborn; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002491:Central Nervous System Agents / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D036145:Ginsenosides / Q000737:chemistry / Q000494:pharmacology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology*; D007150:Immunohistochemistry / Q000379:methods; D007381:Intermediate Filament Proteins / Q000378:metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D051381:Rats; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
D002491:Central Nervous System Agents; D036145:Ginsenosides; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; C577486:Nes protein, rat; D064231:Nestin; D014404:Tubulin; C462941:beta3 tubulin, mouse; D014316:Tritium; D005346:Fibroblast Growth Factors; D001973:Bromodeoxyuridine; C035054:ginsenoside Rg1; D013936:Thymidine