title: The acidic nature of the CcmG redox-active center is important for cytochrome c maturation in Escherichia coli.
abstract: Cytochrome c biogenesis in Escherichia coli is a complex process requiring at least eight genes (ccmABCDEFGH). One of these genes, ccmG, encodes a thioredoxin-like protein with unusually specific redox activity. Here, we investigate the basis for CcmG function and demonstrate the importance of acidic residues surrounding the redox-active center.
authors.affiliation: Institute for Molecular Bioscience, The University of Queensland, Brisbane QLD 4072, Australia.
authors.forename: Melissa A
authors.initials: MA
authors.lastname: Edeling
authors.forename: Umesh
authors.initials: U
authors.lastname: Ahuja
authors.forename: Begoña
authors.initials: B
authors.lastname: Heras
authors.forename: Linda
authors.initials: L
authors.lastname: Thöny-Meyer
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Martin
mesh_terms: D000595:Amino Acid Sequence; D045304:Cytochromes c / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009154:Mutation; D010084:Oxidation-Reduction; D011490:Protein Disulfide Reductase (Glutathione) / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D045304:Cytochromes c; C485980:Ccmg protein, E coli; D011490:Protein Disulfide Reductase (Glutathione)