title: Two-dimensional maps and databases of the human macrophage proteome and secretome.
abstract: Macrophages exert a crucial, but still incompletely known, role in complex disorders such inflammatory, immunological, and infectious diseases. A differential proteomic approach should help to elucidate the macrophage dysfunctions involved in these diseases. With this goal in mind, we established the first two-dimensional maps of the human macrophage proteome and secretome. Intracellular and secreted proteins were extracted from monocyte-derived macrophages obtained from healthy donors (n = 16), and separated by two-dimensional gel electrophoresis. Silver-stained gels were analyzed using Progenesis software. A high level of between-gel reproducibility was obtained, allowing us to generate two patterns specific of the macrophage proteome and secretome, respectively. A total of 127 and 66 distinct intracellular and secreted polypeptide spots, corresponding to 100 and 38 different proteins, respectively, were identified by matrix assisted laser desorption/ionisation-mass spectrometry. The two-dimensional reference maps and databases resulting from this study confirm that macrophages are involved in a wide range of biological functions, and that they provide a useful tool for a wide array of investigators involved in macrophage biology, allowing to investigate the macrophage protein changes associated with various disorders or environmental stimuli.
authors.affiliation: INSERM U508, Institut Pasteur de Lille, Lille, France.
authors.forename: Annabelle
authors.initials: A
authors.lastname: Dupont
authors.forename: Caroline
authors.initials: C
authors.lastname: Tokarski
authors.forename: Olivia
authors.initials: O
authors.lastname: Dekeyzer
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Guihot
authors.forename: Philippe
authors.initials: P
authors.lastname: Amouyel
authors.forename: Christian
authors.initials: C
authors.lastname: Rolando
authors.forename: Florence
authors.initials: F
authors.lastname: Pinet
mesh_terms: D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008264:Macrophages / Q000737:chemistry*; D013058:Mass Spectrometry; D010449:Peptide Mapping / Q000379:methods*; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014018:Tissue Distribution
chemical_list: D011506:Proteins; D020543:Proteome