Cardiac chimerism in recipients of peripheral-blood and bone marrow stem cells.
Multipotent progenitor cells have the ability to differentiate into most somatic cell types, including cardiac myocytes. We sought to investigate cardiac chimerism after peripheral-blood and bone marrow stem cell transplantation. Between 10 and 17 highly polymorphic short tandem repeat (STR) markers were assayed in DNA obtained from donors' peripheral blood, recipients' peripheral blood before transplantation, and the recipient's heart in every patient. Gender and non-gender STR donor alleles were identified in the recipient heart in three patients. Using a highly sensitive PCR assay to determine donor and recipient genotypes, we confirmed the existence of cardiac chimerism in recipients of peripheral-blood and bone marrow stem cells.
Bayes-Genis
Antoni
A
Cardiology Service, Hospital de la Santa Creu i Sant Pau, C/Sant Antoni Ma Claret 167, 08025 Barcelona, Spain. abayesgenis@hsp.santpau.es
Muñiz-Diaz
Eduardo
E
Catasus
Lluis
L
Arilla
Marina
M
Rodriguez
Carmen
C
Sierra
Jordi
J
Madoz
Pedro J
PJ
Cinca
Juan
J
D000328:Adult; D000483:Alleles; D016026:Bone Marrow Transplantation; D005188:False Negative Reactions; D005260:Female; D005838:Genotype; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000628:therapy; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000628:therapy; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000166:cytology; D032383:Myocytes, Cardiac / Q000637:transplantation; D036102:Peripheral Blood Stem Cell Transplantation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020080:Tandem Repeat Sequences / Q000235:genetics; D014184:Transplantation, Homologous; D016896:Treatment Outcome