Cross-linking of P-selectin glycoprotein ligand-1 induces death of activated T cells.
Increasing evidence has shown that death signaling in T cells is regulated in a complicated way. Molecules other than death receptors can also trigger T-cell death. Here, we demonstrate for the first time that P-selectin glycoprotein ligand-1 (PSGL-1) or CD162 molecules cross-linked by an anti-PSGL-1 monoclonal antibody, TAB4, can trigger a death signal in activated T cells. In contrast to classic cell death, PSGL-1-mediated T-cell death is caspase independent. It involves translocation of apoptosis-inducing factor from mitochondria to nucleus and mitochondrial cytochrome c release. Ultrastructurally, both peripheral condensation of chromatin and apoptotic body were observed in PSGL-1-mediated T-cell death. Collectively, this study demonstrates a novel role for PSGL-1 in controlling activated T-cell death and, thus, advances our understanding of immune regulation.
Chen
Shu-Ching
SC
Graduate Institute of Immunology and Department of Anatomy and Cell Biology, Colege of Medicine, National Taiwan University, Taipei, Taiwan.
Huang
Chiu-Chen
CC
Chien
Chung-Liang
CL
Jeng
Chung-Jiuan
CJ
Su
Ho-Ting
HT
Chiang
Evelyn
E
Liu
Meng-Ru
MR
Wu
C H Herbert
CH
Chang
Chung-Nan
CN
Lin
Rong-Hwa
RH
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D051033:Apoptosis Inducing Factor; D016466:CHO Cells; D020169:Caspases / Q000378:metabolism; D016923:Cell Death / Q000276:immunology*; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D006224:Cricetinae; D003432:Cross-Linking Reagents / Q000378:metabolism; D045304:Cytochromes c / Q000378:metabolism; D019040:E-Selectin / Q000378:metabolism; D005420:Flavoproteins / Q000378:metabolism; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D008647:Mesocricetus; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008854:Microscopy, Electron; D008928:Mitochondria / Q000378:metabolism / Q000648:ultrastructure; D019007:P-Selectin / Q000378:metabolism; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism*; D014162:Transfection
D000911:Antibodies, Monoclonal; D051033:Apoptosis Inducing Factor; D003432:Cross-Linking Reagents; D019040:E-Selectin; D005420:Flavoproteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D019007:P-Selectin; C084882:P-selectin ligand protein; C494830:AIFM1 protein, mouse; D045304:Cytochromes c; D020169:Caspases