Rapid detection of quinolone-resistant Salmonella by real time SNP genotyping.
A total of 63 isolates were screened for the gyrA mutation (87Asp-Tyr) in Salmonella enterica serovars using real time PCR. All of the isolates were successfully identified as resistant or susceptible, consistent with the MIC result of the agar dilution method and gyrA sequencing.
Esaki
Hidetake
H
National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1 Tokura, Kokubunji, Tokyo 185-8511, Japan.
Noda
Ken
K
Otsuki
Noriyuki
N
Kojima
Akemi
A
Asai
Tetsuo
T
Tamura
Yutaka
Y
Takahashi
Toshio
T
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D027081:DNA Gyrase / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005456:Fluorescent Dyes / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D015363:Quinolones / Q000494:pharmacology*; D012481:Salmonella Infections, Animal / Q000382:microbiology; D019779:Salmonella enterica / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification
D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D005456:Fluorescent Dyes; D015363:Quinolones; D027081:DNA Gyrase