[Effect of ATRA and DNR on the expression and secretion of VEGF in leukemic cells].
OBJECTIVE
To investigate the expression of VEGF mRNA and secretion of VEGF protein in NB4 and HL-60 cells affected by all-trans retinoic acid (ATRA) and daunorubincin (DNR) respectively.


METHODS
Semi-quantitative RT-PCR and ELISA were used to study the expression of VEGF mRNA and secretion of VEGF protein in NB4 and HL-60 cell lines treated by ATRA and DNR respectively.


RESULTS
VEGF was expressed in both NB4 and HL-60 cells. The expression of VEGF mRNA and secretion of VEGF protein could be down-regulated by ATRA and DNR respectively in a time and dose dependent manner.


CONCLUSIONS
Besides inducing apoptosis and restraining proliferation of leukemic cells, ATRA and DNR exerted their anti-leukemia effects by reducing angiogenesis via reduction of angiogenic reaction stimulating signals.
Wang
Chen
C
Department of Leukemia Research, Shanghai Institute of Hematology, Shanghai 200001, China.
Chen
Fang-Yuan
FY
Gu
Chun-Hong
CH
Han
Jie-Ying
JY
Zhong
Hua
H
Zhong
Ji-Hua
JH
Teng
Ye
Y
Ouyang
Ren-Rong
RR
D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003630:Daunorubicin / Q000494:pharmacology*; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D014212:Tretinoin / Q000494:pharmacology*; D042442:Vascular Endothelial Growth Factors / Q000235:genetics* / Q000378:metabolism*
D042442:Vascular Endothelial Growth Factors; D014212:Tretinoin; D003630:Daunorubicin