A novel method for the preparation of nucleoside triphosphates from activated nucleoside phosphoramidates.
[reaction: see text] A novel method for the preparation of nucleoside triphosphates has been developed. The strategy employs a highly reactive pyrrolidinium phosphoramidate zwitterion intermediate that undergoes efficient coupling with tris(tetra-n-butylammonium) hydrogen pyrophosphate to generate nucleoside triphosphate.
Wu
Weidong
W
Department of Medicinal Chemistry and Molecular Pharmacology and Cancer Center, Purdue University, West Lafayette, Indiana 47907, USA.
Freel Meyers
Caren L
CL
Borch
Richard F
RF
D000577:Amides / Q000138:chemical synthesis / Q000737:chemistry; D011756:Diphosphates / Q000737:chemistry; D009711:Nucleotides / Q000138:chemical synthesis*; D010756:Phosphoric Acids / Q000138:chemical synthesis / Q000737:chemistry; D011759:Pyrrolidines / Q000737:chemistry; D013936:Thymidine / Q000737:chemistry; D013942:Thymine Nucleotides / Q000737:chemistry
D000577:Amides; D011756:Diphosphates; D009711:Nucleotides; D010756:Phosphoric Acids; D011759:Pyrrolidines; D013942:Thymine Nucleotides; C011067:phosphoramidic acid; D013936:Thymidine