[Mechanisms of the effect of brain histamine on chronic epilepsy induced by pentylenetetrazole].
To investigate the mechanisms of histamine on chronic epilepsy induced by pentylenetetrazole (PTZ).



To induce chemical kindling, a subconvulsive dose (35mg/kg) of PTZ was ip injected every 48 h in rats. Behavior changes were observed for 30 min after every injection of PTZ.



Ip injection of histidine or icv injection of clobenpropit inhibited the development of kindling induced by PTZ, presenting prolonged latency for myoclonic jerks and clonic generalized seizures and depressed seizure stages in a dose-dependent manner. H(3)receptor agonist, immepip, and histidine decarboxylase, alpha-fluoromethylhistidine reversed the ameliorating effect of clobenpropit on seizure development in a dose-dependent manner.



Brain histamine plays an important role in protection against myoclonic jerks and clonic generalized clonic seizures and its action may be via H(3)receptor.
Zhang
Li-san
LS
Department of Pharmacology, College of Medicine, Zhejiang University, Hangzhou 310031, China.
Shen
Hai-qing
HQ
Jin
Chun-lei
CL
Hu
Wei-wei
WW
Zhao
Meng-hui
MH
Chen
Zhong
Z
D000818:Animals; D001921:Brain / Q000502:physiology*; D002908:Chronic Disease; D004305:Dose-Response Relationship, Drug; D004827:Epilepsy / Q000139:chemically induced*; D006632:Histamine / Q000502:physiology*; D006639:Histidine / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology; D008297:Male; D010433:Pentylenetetrazole / Q000494:pharmacology*; D010880:Piperidines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013890:Thiourea / Q000031:analogs & derivatives* / Q000494:pharmacology
D007093:Imidazoles; D010880:Piperidines; C085755:4-(1H-imidazol-4-ylmethyl)piperidine; D006639:Histidine; D006632:Histamine; D013890:Thiourea; C086108:clobenpropit; D010433:Pentylenetetrazole