Application of electrospray ionization mass spectrometry in rapid typing of fengycin homologues produced by Bacillus subtilis.
OBJECTIVE
To rapidly type the fengycin homologues produced by Bacillus subtilis strains with electrospray ionization/collision-induced dissociation (ESI/CID) mass spectrometry.


RESULTS
Fengycin homologues produced by Bacillus subtilis JA were analysed. When each homologue was subjected to ESI/CID analysis, ions representing characteristic fragmentations were detected. These ions can help to identify the homologues; even homologues of the same nominal mass can be discriminated by their ESI/CID spectra. Based on the CID results, fengycin homologues can be correctly assigned.
ESI/CID leads to rapid detection and structural characterization of fengycin homologues or lipopeptides with similar properties. It will be very useful in studying the regulatory expression of these peptides.
Wang
J
J
Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Science, Hefei, Anhui, China. wangjun0457@sina.com
Liu
J
J
Wang
X
X
Yao
J
J
Yu
Z
Z
D001412:Bacillus subtilis / Q000737:chemistry / Q000145:classification*; D015373:Bacterial Typing Techniques; D055666:Lipopeptides; D008074:Lipoproteins / Q000737:chemistry / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D055666:Lipopeptides; D008074:Lipoproteins; C049972:fengycin