[Proteomic analysis of proteins involved in the renal phenotype in renovascular hypertension].
Renovascular hypertension is characterised by stenosis of the renal artery and high plasma renin levels due to the recruitment of renin-producing cells along the afferent arterioles. This increase in myoepithelioid cells is mainly a result of the differentiation of existing smooth muscle cells with acquisition of a secretory phenotype. To understand the molecular mechanisms involved in this recruitment, we used the model of renovascular hypertension known as the two-kidney, one-clip model in the Lewis rat. Renal arterioles were isolated using magnetised iron suspension. Differential proteomic analysis was performed using two-dimensional electrophoresis gel followed by mass spectrometry for identification. The most striking protein revealed by proteomics is troponin T, which is down-regulated in the afferent arterioles of the clipped kidney. Confocal microscopy showed that troponin T is specific to the smooth muscle phenotype and absent in the myoepithelioid phenotype.
Pinet
Florence
F
INSERM U508, Institut Pasteur de Lille, Lille, France. florence.pinet@pasteur-lille.fr
Poirier
Florence
F
Fuchs
Sébastien
S
Tharaux
Pierre-Louis
PL
Caron
Michel
M
Corvol
Pierre
P
Michel
Jean-Baptiste
JB
Joubert-Caron
Raymonde
R
D000818:Animals; D001160:Arterioles / Q000378:metabolism / Q000473:pathology; D004591:Electrophoresis, Polyacrylamide Gel; D006978:Hypertension, Renovascular / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D013058:Mass Spectrometry; D010641:Phenotype; D011506:Proteins / Q000235:genetics*; D040901:Proteomics; D051381:Rats; D011917:Rats, Inbred Lew; D012079:Renal Circulation
D011506:Proteins