In vitro semisynthesis and applications of C-terminally modified rab proteins.
Expressed protein ligation is a powerful tool for the generation of natively folded proteins composed of recombinantly generated and chemically synthesized polypeptides. Using this approach, we developed protocols for the production of prenylated and/or otherwise-labeled Rab GTPase. The protocols are generally applicable to most small GTPases that can be supplied with a variety of new chemical functionalities. We used semisynthetic fluorescently labeled Rab7 GTPase as a molecular probe to study protein-protein interactions with components of the prenylation machinery
Durek
Thomas
T
Max-Planck-Institut für Molekulare Physiologie, Dortmund, Germany.
Goody
Roger S
RS
Alexandrov
Kirill
K
D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D011499:Protein Processing, Post-Translational; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013194:Staining and Labeling; D020691:rab GTP-Binding Proteins / Q000138:chemical synthesis* / Q000737:chemistry; D000090802:rab7 GTP-Binding Proteins
D000090802:rab7 GTP-Binding Proteins; D020691:rab GTP-Binding Proteins