The acidic nature of the CcmG redox-active center is important for cytochrome c maturation in Escherichia coli.
Cytochrome c biogenesis in Escherichia coli is a complex process requiring at least eight genes (ccmABCDEFGH). One of these genes, ccmG, encodes a thioredoxin-like protein with unusually specific redox activity. Here, we investigate the basis for CcmG function and demonstrate the importance of acidic residues surrounding the redox-active center.
Edeling
Melissa A
MA
Institute for Molecular Bioscience, The University of Queensland, Brisbane QLD 4072, Australia.
Ahuja
Umesh
U
Heras
Begoña
B
Thöny-Meyer
Linda
L
Martin
Jennifer L
JL
D000595:Amino Acid Sequence; D045304:Cytochromes c / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009154:Mutation; D010084:Oxidation-Reduction; D011490:Protein Disulfide Reductase (Glutathione) / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D029968:Escherichia coli Proteins; D045304:Cytochromes c; C485980:Ccmg protein, E coli; D011490:Protein Disulfide Reductase (Glutathione)