[Effect of HMBA on differentiation and apoptosis of HL-60 and U937 cells and its mechanism].
OBJECTIVE
To explore the effect of hexamethylene bisacetamide (HMBA) on the differentiation and apoptosis of HL-60 and U937 cells, and its mechanism.


METHODS
Flow cytometry was used to evaluate the expressions of cellular surface antigen CD(11b), CD(14), apoptotic marker Annexin V, cell cycle distribution and endocytic antigen cyclin D, cyclin E and p27. Changes of c-myc, Rb, Bcl-2 gene mRNA levels were detected by RT-PCR.


RESULTS
After 72 hours of HMBA treatment, CD(11b) expressions increased significantly, apoptosis increased under high-dose HMBA, cells were arrested in G(0)/G(1) phase and reduced cyclin E, increased cyclin D and p27 were significant in a dose-dependent manner in HL-60 and U937 cells. RT-PCR showed that c-myc and bcl-2 mRNA was significantly down-regulated and Rb mRNA up-regulated in HL-60 and U937 cells.


CONCLUSIONS
HMBA can induce the differentiation of HL-60 and U937 cells, while apoptosis of these cell is induced only by high dose of HMBA. The possible mechanism of HMBA inducing differentiation might be related to the changes of cell cycle regulators and certain proliferation and differentiation related genes.
Wang
Qin-Hong
QH
Haematology Department, Fudan University Affiliated Huashan Hospital, Shanghai 200040, China.
Xie
Yi
Y
Fan
Hua-Hua
HH
Gao
Li
L
Liu
Yan
Y
Xie
Yan-Hui
YH
D000081:Acetamides / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D020298:U937 Cells
D000081:Acetamides; D018797:Cell Cycle Proteins; C014026:hexamethylene bisacetamide