The proton-driven rotor of ATP synthase: ohmic conductance (10 fS), and absence of voltage gating.
The membrane portion of F(0)F(1)-ATP synthase, F(0), translocates protons by a rotary mechanism. Proton conduction by F(0) was studied in chromatophores of the photosynthetic bacterium Rhodobacter capsulatus. The discharge of a light-induced voltage jump was monitored by electrochromic absorption transients to yield the unitary conductance of F(0). The current-voltage relationship of F(0) was linear from 7 to 70 mV. The current was extremely proton-specific (>10(7)) and varied only slightly ( approximately threefold) from pH 6 to 10. The maximum conductance was approximately 10 fS at pH 8, equivalent to 6240 H(+) s(-1) at 100-mV driving force, which is an order-of-magnitude greater than of coupled F(0)F(1). There was no voltage-gating of F(0) even at low voltage, and proton translocation could be driven by deltapH alone, without voltage. The reported voltage gating in F(0)F(1) is thus attributable to the interaction of F(0) with F(1) but not to F(0) proper. We simulated proton conduction by a minimal rotary model including the rotating c-ring and two relay groups mediating proton exchange between the ring and the respective membrane surface. The data fit attributed pK values of approximately 6 and approximately 10 to these relays, and placed them close to the membrane/electrolyte interface.
Feniouk
Boris A
BA
Division of Biophysics, Faculty of Biology/Chemistry, University of Osnabruck, Osnabruck, Germany.
Kozlova
Maria A
MA
Knorre
Dmitry A
DA
Cherepanov
Dmitry A
DA
Mulkidjanian
Armen Y
AY
Junge
Wolfgang
W
D025181:ATP Synthetase Complexes / Q000378:metabolism*; D002856:Chromatophores / Q000201:enzymology*; D003580:Cytochromes / Q000378:metabolism; D006096:Gramicidin / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D015640:Ion Channel Gating / Q000502:physiology*; D008962:Models, Theoretical; D009995:Osmosis / Q000502:physiology; D011522:Protons; D016354:Rhodobacter capsulatus / Q000201:enzymology*; D013053:Spectrophotometry
D003580:Cytochromes; D011522:Protons; D006096:Gramicidin; D025181:ATP Synthetase Complexes