The determination of deoxyribonucleic acids with triadimenol based on the enhancement of resonance light scattering.
For the first time, triadimenol was used to determine nucleic acid (DNA) using the resonance light scattering (RLS) technique. The RLS of triadimenol was greatly enhanced by DNA in the range of pH 1.6 to approximately 1.9. A resonance light-scattering peak at 310 nm was found, and the enhanced intensity of RLS at this wavelength was proportional to the concentration of DNA. The linear range of the calibration curve was 0 to approximately 9 microg/ml with the detection limit of 24 ng ml(-1). The mechanism studies of the system indicated that the enhanced RLS is due to the aggregation of triadimenol on DNA. The nucleic acids in synthetic samples and in rice seedling extraction were analyzed with satisfactory results. Compared with other methods, this method is convenient, rapid, inexpensive and simple.
Jie
Nianqin
N
College of Applied Chemistry, China Agricultural University, Beijing, PR China. aydy-826@163.com
Hou
Shicong
S
Du
Fengpei
F
Zhang
Chunrong
C
Qin
Guoxin
G
D004247:DNA / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D012015:Reference Standards; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D014230:Triazoles
D014230:Triazoles; C032910:triadimefon; D004247:DNA