Analysis of chromosomes 8 and 17 aneuploidies in laryngeal squamous cell carcinoma by fluorescence in situ hybridization.
OBJECTIVE
The objectives were to investigate chromosomes 8 and 17 numerical aberrations by using fluorescence in situ hybridization in laryngeal squamous cell carcinoma and also to determine whether there is any association between chromosomes 8 and 17 aneuploidies and TNM classification and subgroups of laryngeal squamous cell carcinomas.


METHODS
Descriptive study.


METHODS
Tumor and control samples were taken from 23 patients with LSCC by surgical operation. Fluorescence in situ hybridization analysis with chromosomes 8- and 17-specific alpha-satellite DNA probes was performed on the interphase nuclei.


RESULTS
The percentages for chromosomes 8 and 17 aneuploidies were 33% (SD = 25.7%) (median value, 26.2%; range, 3.5%-81.8%) and 19.2% (SD = 15.8%) (median value, 9.8%; range, 3.6%-63.7%), respectively. There was a significant difference between stage 2 and stage 3 (P <.05) and between stage 2 and stage 4 for chromosome 8 aneuploidy (P <.05) but not for chromosome 17 aneuploidy (P >.05). There was also a significant difference for the T classification for chromosome 8 aneuploidy (P <.05) but not for chromosome 17 (P >.05).


CONCLUSIONS
Chromosome 8 aneuploidy may be related to stage and T classification of laryngeal squamous cell carcinoma and its progression.
Oztürk
Kayhan
K
Departments of Otolaryngology, Meram Medical Faculty, Selçuk University, Konya, Turkey. kayhan@medscape.com
Acar
Hasan
H
Durmuş
Ercan
E
Oztürk
Adnan
A
Mutlu
Necip
N
D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D000782:Aneuploidy; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002886:Chromosomes, Human, Pair 17; D002898:Chromosomes, Human, Pair 8; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D018709:Statistics, Nonparametric