title: [Apoptosis and modulatory mechanism of human AHH-1 T lymphocytes induced by gamma-irradiation].
abstract: OBJECTIVE
  To explore the apoptotic characteristics and modulatory mechanism of human AHH-1 T lymphoblast induced by ionizing radiation and provide an experimental basis for preventing and treating immune damage in acute radiation sickness.
  
  
  METHODS
  TdT-mediated dUTP nick end labeling (TUNEL) and May-Grunwald Giemsa (MGG) staining, MTT colorimetry and immunohistochemical staining were used to detect the T lymphocyte apoptosis, cell survival and the expressions of Bax, Bcl-XL and caspase-3 proteins.
  
  
  RESULTS
  (1) Over a definite dose range, apoptotic rate of AHH-1T lymphocytes increased with the elevation of radiation doses, while the lymphocytic survival rate decreased sharply, both showing a good dose-effect relationship. (2) The expression of apoptosis-accelerating protein Bax enhanced obviously with the increase of radiation doses, while apoptosis-inhibiting protein Bcl-XL trended to persistent decline. (3)The activity of activated apoptosis-executing protein caspase-3 heightened significantly with increased radiation doses, which revealed a better corresponding relationship to apoptotic rate.
  
  
  CONCLUSIONS
  A great number of apoptotic AHH-1 T cells may be one of major accounts for the lymphocyte rapid reduction and depressed organism immune function induced by irradiation. Bax, Bcl-XL and caspase-3 proteins play an important role in the regulation of radiation-induced T cell apoptosis.
authors.affiliation: Department of Immunology, Institute of Radiation Medicine, Beijing 100850, China. yufangc@vip.sina.com
authors.forename: Yu-fang
authors.initials: YF
authors.lastname: Cui
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiao-lan
authors.initials: XL
authors.lastname: Liu
authors.forename: Li-hong
authors.initials: LH
authors.lastname: Wang
authors.forename: Zhu-jun
authors.initials: ZJ
authors.lastname: Jiang
authors.forename: Qing-yang
authors.initials: QY
authors.lastname: Gu
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.forename: Han
authors.initials: H
authors.lastname: Xu
authors.forename: Bin-zhi
authors.initials: BZ
authors.lastname: Mao
mesh_terms: D017209:Apoptosis / Q000528:radiation effects*; D053148:Caspase 3; D020169:Caspases / Q000096:biosynthesis; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006801:Humans; D011518:Proto-Oncogene Proteins / Q000032:analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D013601:T-Lymphocytes / Q000166:cytology / Q000528:radiation effects*; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein
chemical_list: C494826:BAX protein, human; C494811:BCL2L1 protein, human; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases