Hyperplasia of dermal microvascular pericytes in scleroderma.
BACKGROUND
Pericytes (PCs) are smooth muscle-like mural cells of capillaries and venules, which can synthesize matrix components and fibroblast-activating cytokines, and are thus potential mediators of pathological changes in scleroderma. In this study, alterations in microvessels were quantitatively imaged, taking PC into account for the first time.


METHODS
Skin biopsies from systemic (12) and localized (14) scleroderma forms as well as age-, sex-, and body location-matched controls were examined with respect to capillary and venular densities as well as endothelial cell (EC) and PC counts using a newly developed (in respect of PC and EC) indirect collagen IV immunostaining-based method.


RESULTS
Hyperplasia of the PC that doubled the microvascular PC density was the most conspicuous characteristic. In the capillaries of the upper dermal plexus of the periphery of the sclerotic zones, median ratios of PC : EC were 0.23 (controls 0.10) or 0.18 (controls 0.11) in systemic or localized scleroderma, respectively. Furthermore, an increase in capillary density in the upper dermal plexus could be demonstrated in the marginal zones of both types of disease.


CONCLUSIONS
The observed PC increase in the peripheral zones of active disease supports the hypothesis of a vascular pathogenesis of scleroderma and directs the focus to microvascular PC.
Helmbold
Peter
P
Universitätsklinik und Poliklinik für Dermatologie und Venerologie der Martin-Luther-Universität Halle - Wittenberg, Halle (Saale), Germany. peter.helmbold@medizin.uni-halle.de
Fiedler
Eckhard
E
Fischer
Matthias
M
Marsch
Wolfgang Ch
WCh
D000293:Adolescent; D000328:Adult; D000368:Aged; D002196:Capillaries / Q000378:metabolism / Q000473:pathology; D002452:Cell Count; D024141:Collagen Type IV / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D006965:Hyperplasia / Q000473:pathology*; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008875:Middle Aged; D020286:Pericytes / Q000473:pathology*; D012594:Scleroderma, Localized / Q000473:pathology*; D012595:Scleroderma, Systemic / Q000473:pathology*; D012867:Skin / Q000098:blood supply* / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000378:metabolism
D024141:Collagen Type IV; D016212:Transforming Growth Factor beta