title: DLEU2 encodes an antisense RNA for the putative bicistronic RFP2/LEU5 gene in humans and mouse.
abstract: Our group previously identified two novel genes, RFP2/LEU5 and DLEU2, within a 13q14.3 genomic region of loss seen in various malignancies. However, no specific inactivating mutations were found in these or other genes in the vicinity of the deletion, suggesting that a nonclassical tumor-suppressor mechanism may be involved. Here, we present data showing that the DLEU2 gene encodes a putative noncoding antisense RNA, with one exon directly overlapping the first exon of the RFP2/LEU5 gene in the opposite orientation. In addition, the RFP2/LEU5 transcript can be alternatively spliced to produce either several monocistronic transcripts or a putative bicistronic transcript encoding two separate open-reading frames, adding to the complexity of the locus. The finding that these gene structures are conserved in the mouse, including the putative bicistronic RFP2/LEU5 transcript as well as the antisense relationship with DLEU2, further underlines the significance of this unusual organization and suggests a biological function for DLEU2 in the regulation of RFP2/LEU5.
authors.affiliation: Molecular Biology Laboratory, Royal Bournemouth Hospital, Bournemouth, United Kingdom.
authors.forename: Martin M
authors.initials: MM
authors.lastname: Corcoran
authors.forename: Marianne
authors.initials: M
authors.lastname: Hammarsund
authors.forename: Chaoyong
authors.initials: C
authors.lastname: Zhu
authors.forename: Mikael
authors.initials: M
authors.lastname: Lerner
authors.forename: Bagrat
authors.initials: B
authors.lastname: Kapanadze
authors.forename: Bill
authors.initials: B
authors.lastname: Wilson
authors.forename: Catharina
authors.initials: C
authors.lastname: Larsson
authors.forename: Lars
authors.initials: L
authors.lastname: Forsberg
authors.forename: Rachel E
authors.initials: RE
authors.lastname: Ibbotson
authors.forename: Stefan
authors.initials: S
authors.lastname: Einhorn
authors.forename: David G
authors.initials: DG
authors.lastname: Oscier
authors.forename: Dan
authors.initials: D
authors.lastname: Grandér
authors.forename: Olle
authors.initials: O
authors.lastname: Sangfelt
mesh_terms: D017398:Alternative Splicing / Q000235:genetics; D000818:Animals; D001482:Base Composition / Q000235:genetics; D045744:Cell Line, Tumor; D002875:Chromosomes / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology; D005796:Genes / Q000235:genetics*; D005807:Genes, Overlapping; D006367:HeLa Cells; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D007668:Kidney / Q000166:cytology / Q000196:embryology; D051379:Mice; D016366:Open Reading Frames / Q000235:genetics; D011506:Proteins / Q000235:genetics* / Q000502:physiology; D016372:RNA, Antisense / Q000235:genetics*; D062085:RNA, Long Noncoding; D012333:RNA, Messenger / Q000235:genetics; D022661:RNA, Untranslated / Q000235:genetics; D014166:Transferases; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: C109420:DLEU2 lncRNA, human; D004268:DNA-Binding Proteins; D011506:Proteins; D016372:RNA, Antisense; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D022661:RNA, Untranslated; C112217:TRIM13 protein, human; D025521:Tumor Suppressor Proteins; C486375:Dleu2 protein, mouse; D014166:Transferases