[Anti-endotoxin core glycolipid antibody: the preparation of immune serum of E. Coli J5].
OBJECTIVE
To prepare high titer anti-endotoxin core glycolipid (J5) antibody (CGL) for the treatment of Gram-negative bacteremia and septic shock.


METHODS
Nontoxic bacterial vaccine (50 x 10(12)U/L) against E.Coli O111:B4 mutant strain J5 was prepared. J5 bacterial vaccine was injected into rabbits through ear marginal vein (saline as control preparation), one time pre three days, totally five times. Injected doses were as following: 0.1 ml, 0.2 ml, 0.4 ml, 0.6 ml, and 0.8 ml. One week after fifth injection, blood samples from heart were collected and immune serum was isolated. Indirect clotting test was used to determine the titer of antibody and cross reaction.


RESULTS
Among 12 immunized rabbits, titers of antibody against E. Coli J5 were exceeding 1:1 024 in 6 rabbits, and they had cross reaction with various kinds of Gram-negative bacterial endotoxins.


CONCLUSIONS
The titer of anti-endotoxin core glycolipid (E.Coli J5) antibody prepared by us appears to be high, and it can combine with various kinds of Gram-negative bacterial endotoxins.
Xu
Xin-Nu
XN
Key Lab for Critical Care Medicine of the Ministry of Health, First Central Hospital, Tianjin 300192, China.
Li
Zhi-Jun
ZJ
Li
Yin-Ping
YP
D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology; D004731:Endotoxins / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology*; D006017:Glycolipids / Q000276:immunology; D007106:Immune Sera / Q000302:isolation & purification; D007136:Immunoglobulins / Q000302:isolation & purification*; D011817:Rabbits
D001428:Bacterial Vaccines; D004731:Endotoxins; D006017:Glycolipids; D007106:Immune Sera; D007136:Immunoglobulins; C042866:antilipopolysaccharide antibodies; C042058:core endotoxin