Cathepsins K, L, B, X and W are differentially expressed in normal and chronically inflamed gastric mucosa.
The expression of cathepsins K, L, B, X and W was studied by quantitative RT-PCR in normal and inflamed gastric mucosa (antrum, corpus, cardia). Cathepsins B, L, K and X were expressed ubiquitously. In contrast, cathepsin W was expressed at very low levels. Infection by Helicobacter pylori caused a significant induction of cathepsin X (p<0.008), whereas the other cathepsins were not or only locally affected by H. pylori infection or reflux disease. Immunohistochemistry revealed specific expression of cathepsin X (macrophages), cathepsin K (parietal cells) and cathepsin W (lymphocytes), whereas cathepsins B and L were predominantly expressed in epithelial cells.
Bühling
Frank
F
Institute of Immunology, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, D-39120 Magdeburg, Germany.
Peitz
Ulrich
U
Krüger
Sabine
S
Küster
Dörthe
D
Vieth
Michael
M
Gebert
Ingrid
I
Roessner
Albert
A
Weber
Ekkehard
E
Malfertheiner
Peter
P
Wex
Thomas
T
D002401:Cathepsin B / Q000235:genetics / Q000378:metabolism; D056657:Cathepsin K; D056668:Cathepsin L; D056664:Cathepsin W; D002403:Cathepsins / Q000235:genetics / Q000378:metabolism*; D002908:Chronic Disease; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism; D005753:Gastric Mucosa / Q000201:enzymology* / Q000473:pathology; D005756:Gastritis / Q000201:enzymology* / Q000382:microbiology / Q000473:pathology; D005764:Gastroesophageal Reflux / Q000201:enzymology; D015870:Gene Expression; D016481:Helicobacter Infections / Q000201:enzymology* / Q000473:pathology; D006801:Humans
D002403:Cathepsins; C534187:CTSW protein, human; D056664:Cathepsin W; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; C534281:CTSL protein, human; D056668:Cathepsin L; C534130:CTSK protein, human; D056657:Cathepsin K