Effect of technological processing on the allergenicity of mangoes (Mangifera indica L.).
In parallel with the rising popularity of exotic fruits in Europe, allergy against mango is of increasing importance. Because mangoes are also consumed as processed products such as chutneys or beverages, the influences of different process conditions on their allergenicity were investigated. Mango purees and nectars were manufactured at small pilot-plant scale, and the allergenic potencies of the resulting intermediate and final products were determined by means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), immunoblotting and inhibitive enzyme allergosorbent tests (EAST-inhibition), using a pool serum of 9 individuals with manifest mango allergy. The mango allergens were shown to be very stable during technological processing. Irrespective of enzymatic matrix decomposition, mechanical tissue disintegration and heating during peeling, mash treatment, and pasteurization, significant loss of allergenicity could not be observed in the extracts of mango purees and nectars derived thereof. These results were confirmed by analogous investigation of commercial mango drinks and nectars. Hence, conventional mango processing into pulp-containing products typical for this species obviously does not allow complete elimination of the allergenic potency.
Dube
Mark
M
Institute of Biochemistry and Food Chemistry, Section of Food Chemistry, University of Hamburg, Grindelallee 117, D-20146 Hamburg, Germany.
Zunker
Katy
K
Neidhart
Sybille
S
Carle
Reinhold
R
Steinhart
Hans
H
Paschke
Angelika
A
D000485:Allergens / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005511:Food Handling; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000097:blood; D031022:Mangifera / Q000276:immunology*; D010936:Plant Extracts / Q000276:immunology
D000485:Allergens; D010936:Plant Extracts; D007073:Immunoglobulin E