title: Antagonism of nitrous oxide antinociception in mice by antisense oligodeoxynucleotide directed against neuronal nitric oxide synthase enzyme.
abstract: Nitric oxide (NO) has been implicated in the antinociceptive effect of nitrous oxide (N2O) in mice. This study was conducted to determine the sensitivity of N2O-induced antinociception to antagonism by an antisense oligodeoxynucleotide (AS-ODN) against neuronal nitric oxide synthase (nNOS). The AS-ODN significantly antagonized the antinociceptive effect of N2O in the abdominal constriction test, but a mismatch ODN was without effect. This result implicates the specific involvement of nNOS in N2O-induced antinociception.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, Pullman 99164-6534, USA.
authors.forename: Shuang
authors.initials: S
authors.lastname: Li
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Bieber
authors.forename: Raymond M
authors.initials: RM
authors.lastname: Quock
mesh_terms: D000700:Analgesics / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D004347:Drug Interactions; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000235:genetics; D052248:Nitric Oxide Synthase Type I; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D010147:Pain Measurement / Q000187:drug effects
chemical_list: D000700:Analgesics; D020319:Oligodeoxyribonucleotides, Antisense; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496321:Nos1 protein, mouse