title: A novel method of Multiplexed Competitive Antibody Binning for the characterization of monoclonal antibodies.
abstract: We have developed a novel method of high-throughput Multiplexed Competitive Antibody Binning (MCAB). Using only a small amount of antibody and antigen, this method enables the sorting of a large, complex panel of monoclonal antibodies into different bins based on cross-competition for antigen binding. The MCAB assay builds on Luminex multiplexing bead-based technology to detect antibody competition. Because of its high sensitivity, the MCAB method is immediately applicable after identification of antigen-positive mAbs, providing information useful for advancing mAb candidates into further testing. The MCAB assay also can be used for sorting mAbs into binding groups after screening for functional activity.
authors.affiliation: Abgenix, Inc., 6701 Kaiser Drive, Fremont, CA 94555, USA.
authors.forename: Xiao-Chi
authors.initials: XC
authors.lastname: Jia
authors.forename: Robert
authors.initials: R
authors.lastname: Raya
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Orit
authors.initials: O
authors.lastname: Foord
authors.forename: Wynn L
authors.initials: WL
authors.lastname: Walker
authors.forename: Michael L
authors.initials: ML
authors.lastname: Gallo
authors.forename: Mary
authors.initials: M
authors.lastname: Haak-Frendscho
authors.forename: Larry L
authors.initials: LL
authors.lastname: Green
authors.forename: C Geoffrey
authors.initials: CG
authors.lastname: Davis
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology*; D001666:Binding Sites, Antibody / Q000276:immunology; D001667:Binding, Competitive / Q000276:immunology*; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology; D006801:Humans; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes