title: Overexpression of LI-cadherin in gastric cancer is associated with lymph node metastasis.
abstract: Gastric cancer remains the second leading cause of cancer deaths worldwide. Patients usually present late with local invasion or metastatic diseases. The present study investigated the expression level of liver-intestine cadherin (LI-cadherin) by RT-PCR and its correlation with clinicopathological data in 71 pairs of tumor and non-cancerous gastric mucosa. Protein expression level of LI-cadherin was determined by Western blotting and immunohistochemistry. The mRNA of LI-cadherin was highly expressed in tumor as compared to non-cancerous mucosa. Lymph node metastasis was significantly associated with the expression of LI-cadherin (p=0.038). On multivariate analysis, T staging and LI-cadherin expression were found to be independent factors associated with lymph node metastasis.
authors.affiliation: Department of Surgery, University of Hong Kong Medical Center, Queen Mary Hospital, Hong Kong.
authors.forename: Samuel
authors.initials: S
authors.lastname: Ko
authors.forename: Kent-Man
authors.initials: KM
authors.lastname: Chu
authors.forename: John M
authors.initials: JM
authors.lastname: Luk
authors.forename: Bonnie W
authors.initials: BW
authors.lastname: Wong
authors.forename: Siu-Tsan
authors.initials: ST
authors.lastname: Yuen
authors.forename: Suet-Yi
authors.initials: SY
authors.lastname: Leung
authors.forename: John
authors.initials: J
authors.lastname: Wong
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D015153:Blotting, Western; D015820:Cadherins; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis / Q000235:genetics*; D008297:Male; D026901:Membrane Transport Proteins; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C490584:CDH17 protein, human; D015820:Cadherins; D002352:Carrier Proteins; D017931:DNA Primers; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; C067802:intestinal peptide-proton cotransporter