Ruling out Bacillus anthracis.
Optimization of methods for ruling out Bacillus anthracis leads to increased yields, faster turnaround times, and a lighter workload. We used 72 environmental non-B. anthracis bacilli to validate methods for ruling out B. anthracis. Most effective were the use of horse blood agar, motility testing after isolates had a 2-h incubation in trypticase soy broth, and screening isolates with a B. anthracis-selective agar.
Papaparaskevas
Joseph
J
National and Kapodistrian University of Athens Medical School, Athens, Greece.
Houhoula
Dimitra P
DP
Papadimitriou
Maria
M
Saroglou
Georgios
G
Legakis
Nicholas J
NJ
Zerva
Loukia
L
D000818:Animals; D001408:Bacillus anthracis / Q000302:isolation & purification*; D023482:Bioterrorism; D003470:Culture Media; D006461:Hemolysis; D006801:Humans; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies
D003470:Culture Media