[Changes of telomerase activity and hTERT mRNA expression in the process of apoptosis of HL-60 cells induced by arsenic sulfide].
OBJECTIVE
To explore the effect of arsenic sulfide on telomerase activity of HL-60 cells.


METHODS
Telomerase activity was determined by PCR-ELISA. The expression of hTERT-mRNA was analyzed by semi-quantitative RT-PCR. Flow cytometry was used to analyze the cell cycle,apoptosis and CD11b expression of HL-60 cells.


RESULTS
Treament of HL-60 cells with 0.3-0.6 mg/L arsenic sulfide for 72 h did not induce apoptosis of the cells, while 1.5-3.0 mg/L did. Furthermore, 1.5-3.0 mg/L arsenic sulfide inhibited the telomerase activity and hTERT-mRNA expression in HL-60 cells. Proportion of the cells in G2/M phase was increased when being treated with arsenic sulfide. 3.0 mg/L arsenic sulfide upregulated CD11b expression in HL-60 cells from 1.0 % to 6.8%.


CONCLUSIONS
Arsenic sulfide can inhibit telomerase activity. The increase of proportion of HL-60 cells in G2/M phase may be related to the reduction of telomerase activity. High dosage of arsenic sulfide can slightly induce differentiation of HL-60 cells.
Li
Jing
J
Department of Hematology, First Hospital of Xi'an Jiaotong University, Xi'an 710061, China. anlj1676@eyou.com
Liu
Shan-xi
SX
D017209:Apoptosis / Q000187:drug effects*; D001152:Arsenicals / Q000494:pharmacology*; D039481:CD11b Antigen / Q000032:analysis; D002453:Cell Cycle / Q000187:drug effects; D004268:DNA-Binding Proteins; D018922:HL-60 Cells; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis*; D013440:Sulfides / Q000494:pharmacology*; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism*
D001152:Arsenicals; D039481:CD11b Antigen; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D013440:Sulfides; C045816:arsenic trisulfide; D019098:Telomerase