title: Accumulation of 8-nitroguanine in human gastric epithelium induced by Helicobacter pylori infection.
abstract: Helicobacter pylori infection causes chronic inflammation, which can lead to gastric carcinoma. A double immunofluorescence labeling study demonstrated that the level of 8-nitroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) apparent in gastric gland epithelium was significantly higher in gastritis patients with H. pylori infection than in those without infection. A significant accumulation of proliferating cell nuclear antigen, a prognostic factor for gastric cancer, was observed in gastric gland epithelial cells in patients with H. pylori infection as compared to those without infection, and its accumulation was closely correlated with the formation of 8-nitroguanine and 8-oxodG. These results suggest that nitrosative and oxidative DNA damage in gastric epithelial cells and their proliferation by H. pylori infection may lead to gastric carcinoma. 8-Nitroguanine could be not only a promising biomarker for inflammation but also a useful indicator of the risk of gastric cancer development in response to chronic H. pylori infection.
authors.affiliation: Department of Anatomy, Mie University School of Medicine, Tsu, Mie 514-8507, Japan.
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Adachi
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Hiraku
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Horiki
authors.forename: Shinichirou
authors.initials: S
authors.lastname: Horiike
authors.forename: Ichiro
authors.initials: I
authors.lastname: Imoto
authors.forename: Somchai
authors.initials: S
authors.lastname: Pinlaor
authors.forename: Mariko
authors.initials: M
authors.lastname: Murata
authors.forename: Reiji
authors.initials: R
authors.lastname: Semba
authors.forename: Shosuke
authors.initials: S
authors.lastname: Kawanishi
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism* / Q000382:microbiology; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism*; D016481:Helicobacter Infections / Q000378:metabolism* / Q000382:microbiology; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism
chemical_list: C095838:8-nitroguanine; D018809:Proliferating Cell Nuclear Antigen; D006147:Guanine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine