Detection of griseofulvin in a marine strain of Penicillium waksmanii by ion trap mass spectrometry.
A marine strain of Penicillium waksmanii Zaleski was isolated from a sample of seawater from shellfish-farming area in the Loire estuary (France). The in vitro marine culture showed an important antifungal activity. Bioassay-guided fractionation was used to purify the crude extract. Dereplication by electrospray-ion trap/mass spectrometry (ESI-IT/MS) afforded the identification of the antifungal compound, after a semi-purification consisting of two stages. A comparison of the ionic composition between the active and the non-active fractions allowed the detection of a monocharged ion at m/z 353 containing a chlorine atom, which could be attributed to the antifungal griseofulvin [C17H17ClO6+H]+. Multi-stage fragmentation (MSn) confirmed the identity of the m/z 353 ion of the antifungal fraction as griseofulvin. It is the first description of griseofulvin production by a strain of P. waksmanii and the first chemical study of a strain of this species isolated from marine temperate cold water.
Petit
K E
KE
SMAB (Substances Marines à Activité Biologique), Université de Nantes, ISOMer, Faculté de Pharmacie, BP 53 508, 44035 Nantes cedex 01, France.
Mondeguer
F
F
Roquebert
M F
MF
Biard
J F
JF
Pouchus
Y F
YF
D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006118:Griseofulvin / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D010407:Penicillium / Q000737:chemistry / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology*; D021241:Spectrometry, Mass, Electrospray Ionization; D014871:Water Microbiology
D000935:Antifungal Agents; D006118:Griseofulvin