[Specific promoter activity of CIITA-pI in dendritic cells].
OBJECTIVE
To detect whether CIITA-pI can drive reporter gene expression and its specific promoter activity in DCs.


METHODS
Luciferase reporter system was used to detect 112 bp CIITA-pI specific promoter activity in DCs.


RESULTS
CIITA-pI could drive expression of reporter gene in DCs but not in HEK 293 cells and its promoter activity increased with the maturation of DCs.


CONCLUSIONS
Our work provides a basis for further study of targeting DC gene therapy using CIITA-pI as DC-specific promoter.
Zhang
Yan
Y
Department of Laboratory Diagnosis, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China.
Shen
Qian
Q
D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003713:Dendritic Cells / Q000378:metabolism*; D005802:Genes, MHC Class II; D015316:Genetic Therapy; D006801:Humans; D008156:Luciferases / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009687:Nuclear Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D015534:Trans-Activators / Q000235:genetics*
C083728:MHC class II transactivator protein; D009687:Nuclear Proteins; D015534:Trans-Activators; D008156:Luciferases