Essential role for poly (ADP-ribosyl)ation in mouse preimplantation development.
BACKGROUND
Poly (ADP-ribosyl)ation is a covalent modification of many nuclear proteins. It has a strong chromatin modifying potential involved in DNA repair, transcription and replication. Its role during preimplantation development is unknown.


RESULTS
We have observed strong but transient synthesis of poly ADP-ribose polymers on decondensing chromosomes of fertilized and parthenogenetically activated mouse oocytes. Inhibition of this transient upregulation with a specific enzyme inhibitor, 3-aminobenzamide, has long-term effects on the postimplantation development of the embryos. In addition, inhibition of poly (ADP-ribosyl)ation at the 4-8 cell stage selectively blocks morula compaction.


CONCLUSIONS
These observations suggest that poly (ADP-ribosyl)ation is involved in the epigenetic chromatin remodeling in the zygote.
Imamura
Takuya
T
INSERM E367, Institut Jacques Monod; 2, place Jussieu, 75005 Paris France. imamura@nibb.ac.jp
Neildez
Thi My Anh
TM
Thenevin
Catherine
C
Paldi
Andras
A
D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D001755:Blastocyst / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly / Q000502:physiology; D003433:Crosses, Genetic; D004622:Embryo, Mammalian / Q000737:chemistry / Q000196:embryology; D005314:Embryonic and Fetal Development / Q000502:physiology*; D044127:Epigenesis, Genetic / Q000187:drug effects / Q000502:physiology; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009028:Morula / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D009865:Oocytes / Q000737:chemistry / Q000201:enzymology / Q000254:growth & development; D011064:Poly Adenosine Diphosphate Ribose / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000502:physiology; D011108:Polymers / Q000378:metabolism; D015053:Zygote / Q000201:enzymology / Q000254:growth & development / Q000502:physiology
D001549:Benzamides; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011108:Polymers; D011064:Poly Adenosine Diphosphate Ribose; C025160:3-aminobenzamide; D011065:Poly(ADP-ribose) Polymerases