title: Methods evaluating vanadium tolerance in bacteria isolated from crude oil contaminated land.
abstract: Investigations into bacterial responses to vanadium are rare, and in this study were initiated by isolating cultures from crude oil contaminated soil from Russia and Saudi Arabia. Addition of vanadyl sulphate and vanadium pentoxide created acid conditions in the media whilst sodium metavanadate and sodium orthovanadate produced neutral and alkaline effects, respectively. Buffers were introduced for wider comparison of the sample set treatments and to distinguish between the effects of pH and compound toxicity. This study has resulted in the creation of protocols for the pH stabilisation of media containing vanadium compounds and revealed that, although vanadium salts demonstrated some toxic effects, as revealed by MIC and bioluminescence decay tests, the effects were mainly due to pH rather than inherent toxicity of the metal. Capacity for sorption of vanadium to biomass was also investigated.
authors.affiliation: School of Life Sciences, Napier University, 10 Colinton Road, Edinburgh EH10 5DT, Scotland, UK.
authors.forename: Jennifer M L
authors.initials: JM
authors.lastname: Bell
authors.forename: Jim C
authors.initials: JC
authors.lastname: Philp
authors.forename: Maria S
authors.initials: MS
authors.lastname: Kuyukina
authors.forename: Irena B
authors.initials: IB
authors.lastname: Ivshina
authors.forename: Sandra A
authors.initials: SA
authors.lastname: Dunbar
authors.forename: Colin J
authors.initials: CJ
authors.lastname: Cunningham
authors.forename: Peter
authors.initials: P
authors.lastname: Anderson
mesh_terms: D001419:Bacteria / Q000187:drug effects* / Q000302:isolation & purification; D015169:Colony Count, Microbial; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements; D010084:Oxidation-Reduction; D010578:Petroleum; D012988:Soil Microbiology; D012989:Soil Pollutants; D014639:Vanadium / Q000494:pharmacology*
chemical_list: D010578:Petroleum; D012989:Soil Pollutants; D014639:Vanadium