title: Jelleines: a family of antimicrobial peptides from the Royal Jelly of honeybees (Apis mellifera).
abstract: Four antimicrobial peptides were purified from Royal Jelly of honeybees, by using reverse phase-HPLC and sequenced by using Q-Tof-MS/MS: PFKLSLHL-NH(2) (Jelleine-I), TPFKLSLHL-NH(2) (Jelleine-II), EPFKLSLHL-NH(2) (Jelleine-III), and TPFKLSLH-NH(2) (Jelleine-IV). The peptides were synthesized on-solid phase, purified and submitted to different biological assays: antimicrobial activity, mast cell degranulating activity and hemolysis. The Jelleines-I-III presented exclusively antimicrobial activities against yeast, Gram+ and Gram- bacteria; meanwhile, Jelleine-IV was not active in none of the assays performed. These peptides do not present any similarity with the other antimicrobial peptides from the honeybees; they are produced constitutively by the workers and secreted into Royal Jelly.
authors.affiliation: University of Santa Cruz, Ilhéus, BA, Brazil.
authors.forename: Renato
authors.initials: R
authors.lastname: Fontana
authors.forename: Maria Anita
authors.initials: MA
authors.lastname: Mendes
authors.forename: Bibiana Monson
authors.initials: BM
authors.lastname: de Souza
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Konno
authors.forename: Lílian Mari Marcondes
authors.initials: LM
authors.lastname: César
authors.forename: Osmar
authors.initials: O
authors.lastname: Malaspina
authors.forename: Mario Sergio
authors.initials: MS
authors.lastname: Palma
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001516:Bees / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000737:chemistry*; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D009842:Oligopeptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D021241:Spectrometry, Mass, Electrospray Ionization; D015003:Yeasts / Q000187:drug effects; D000099219:Royal Jelly
chemical_list: D023181:Antimicrobial Cationic Peptides; D005227:Fatty Acids; D009842:Oligopeptides; D000099219:Royal Jelly; C495931:Jelleine I; C495932:Jelleine II; C495933:Jelleine III