Cytotoxicity and oncostatic activity of the thiazolidinedione derivative CGP 52608 on central nervous system cancer cells.
Oncostatic activity of CGP 52608 at concentrations 1 microM or lower, and cytotoxicity at 5 and 10 microM, are showed in central nervous system cancer cell lines. Inhibition of cell growth is accompanied by arrest of the cell cycle in G0/G1 or G2/M, depending on the cells, and suggests different intracellular pathways used on each cell type. Cytotoxicity is not mediated by oxidative stress, since no intra or extracellular peroxides were found after treatment and antioxidants failed to rescue cells from apoptosis induced by CGP 52608. Its use as anticancer drug susceptible of being concomitantly administered with antioxidants must be considered provided its antitumoral effects do not rely on free radical production.
Herrera
Federico
F
Departamento de Morfología y Biología Celular, Facultad de Medicina, c/Julian Claveria, 33006 Oviedo, Spain.
Mayo
Juan C
JC
Martín
Vanesa
V
Sainz
Rosa M
RM
Antolin
Isaac
I
Rodriguez
Carmen
C
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D053148:Caspase 3; D020169:Caspases; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D016543:Central Nervous System Neoplasms / Q000188:drug therapy* / Q000473:pathology; D004354:Drug Screening Assays, Antitumor; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D018384:Oxidative Stress; D016716:PC12 Cells; D051381:Rats; D013844:Thiazoles / Q000008:administration & dosage / Q000494:pharmacology*; D013882:Thiosemicarbazones / Q000008:administration & dosage / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D013844:Thiazoles; D013882:Thiosemicarbazones; C092451:CGP 52608; C505407:CASP3 protein, human; C505409:Casp3 protein, mouse; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases