title: Optimization of procedures for isolation of mycobacteria from soil and water samples obtained in northern India.
abstract: For isolation of environmental mycobacteria, a decontamination procedure has been standardized by which treatment with 3% sodium dodecyl sulfate plus 4% NaOH (15 and 30 min for rapid and slow growers, respectively) is followed by incubation with 2% cetrimide (5 and 15 min for fast- and slow-growing mycobacteria, respectively); this procedure was found to completely eliminate contamination with other organisms and resulted in the isolation of only mycobacteria.
authors.affiliation: Central JALMA Institute for Leprosy and Other Mycobacterial Diseases, Indian Council of Medical Research, Tajganj, Agra 28201, India.
authors.forename: Deepti
authors.initials: D
authors.lastname: Parashar
authors.forename: D S
authors.initials: DS
authors.lastname: Chauhan
authors.forename: V D
authors.initials: VD
authors.lastname: Sharma
authors.forename: Aradhana
authors.initials: A
authors.lastname: Chauhan
authors.forename: S V S
authors.initials: SV
authors.lastname: Chauhan
authors.forename: V M
authors.initials: VM
authors.lastname: Katoch
mesh_terms: D001431:Bacteriological Techniques; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology; D004203:Disinfection / Q000379:methods; D007194:India; D009170:Nontuberculous Mycobacteria / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D012972:Sodium Hydroxide / Q000494:pharmacology; D012988:Soil Microbiology; D014871:Water Microbiology
chemical_list: D002593:Cetrimonium Compounds; D012967:Sodium Dodecyl Sulfate; D012972:Sodium Hydroxide; D000077286:Cetrimonium