A 9 angstroms single particle reconstruction from CCD captured images on a 200 kV electron cryomicroscope.
Sub-nanometer resolution structure determination is becoming a common practice in electron cryomicroscopy of macromolecular assemblies. The data for these studies have until now been collected on photographic film. Using cytoplasmic polyhedrosis virus (CPV), a previously determined structure, as a test specimen, we show the feasibility of obtaining a 9 angstroms structure from images acquired from a 4 k x 4 k Gatan CCD on a 200 kV electron cryomicroscope. The match of the alpha-helices in the protein components of the CPV with the previous structure of the same virus validates the suitability of this type of camera as the recording media targeted for single particle reconstructions at sub-nanometer resolution.
Booth
Christopher R
CR
Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, TX 77030, USA.
Jiang
Wen
W
Baker
Matthew L
ML
Zhou
Z Hong
ZH
Ludtke
Steven J
SJ
Chiu
Wah
W
D020285:Cryoelectron Microscopy / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D012087:Reoviridae / Q000737:chemistry / Q000648:ultrastructure; D014771:Virion / Q000737:chemistry* / Q000648:ultrastructure*