title: Insulin cell mass is altered in Csf1op/Csf1op macrophage-deficient mice.
abstract: Macrophages play an important role in organ development, tissue homeostasis, and remodeling. Thus, we monitored the presence of F4/80-positive macrophages in the pancreas of wild-type mice, and some developmental features of this complex tissue were compared throughout life in wild-type and macrophage-deficient Csf1op/Csf1op (op/op) mice. The combined use of immunohistochemistry, morphometry, and cell quantification allows us to evaluate insulin and glucagon cell mass, total and insulin cell proliferation, and apoptosis in fetuses (E18.5), weanings (postnatal day 21), nonpregnant adults, and adults in late pregnancy (18.5 days). F4/80-positive macrophages were found in pancreases recovered from Csf1op/Csf1+ (op/+) mice but were extremely scarce or absent in pancreas recovered from op/op ones at all studied time-points. The macrophage-deficient op/op phenotype was clearly associated with a major insulin mass deficit in fetuses and adults, abnormal postnatal islet morphogenesis, and impaired pancreatic cell proliferation at weaning and late pregnancy. We also obtained indirect evidence of increased neogenesis in this model at time-points when pancreatic remodeling does occur. The demonstration of the colony-stimulating factor 1-dependent macrophage involvement in life-time pancreas development/remodeling allows us to pinpoint the tissue-modeling and remodeling functions of this leukocyte lineage.
authors.affiliation: INSERM U457, Paris, France.
authors.forename: Linda
authors.initials: L
authors.lastname: Banaei-Bouchareb
authors.forename: Valerie
authors.initials: V
authors.lastname: Gouon-Evans
authors.forename: Dinane
authors.initials: D
authors.lastname: Samara-Boustani
authors.forename: Marie Claire
authors.initials: MC
authors.lastname: Castellotti
authors.forename: Paul
authors.initials: P
authors.lastname: Czernichow
authors.forename: Jeffrey W
authors.initials: JW
authors.lastname: Pollard
authors.forename: Michel
authors.initials: M
authors.lastname: Polak
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D007515:Islets of Langerhans / Q000378:metabolism*; D016173:Macrophage Colony-Stimulating Factor / Q000172:deficiency / Q000235:genetics*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice
chemical_list: D016173:Macrophage Colony-Stimulating Factor