[Immunobiological properties of monoclonal antibodies to Mycoplasma hominis antigens].
Approaches to obtaining stable mouse hybridomas synthesizing monoclonal antibodies (McAb) to M. hominis key antigens were developed. 4 clones capable of the stable synthesis of McAb of different IgG classes were obtained. Clones A3/2 and A5/D produced antibodies to the thermostable determinant to with a mol. wt. of 80-120 kD, sensitive to sodium periodate and resistant to potassium proteinase. Clone H9/B2 synthesized McAb which interacted with potassium proteinase-sensitive M. hominis thermolabile determinant with a mol. wt. of 80 kD. McAb of clone A3/2, labeled with fluorescein isothiocyanate and horse-radish peroxidase, specifically reacted with M. hominis antigens in the immunofluorescence test and the immunoenzyme assay (EIA). The sensitivity of EIA was 0.25 ng/ml of antigen protein. These data may serve as prerequisites for the development of diagnostic test systems aimed at the detection of M. hominis antigens in different clinical substances.
Gorina
L G
LG
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia.
Kiseleva
T B
TB
Drobyshevskaia
E I
EI
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology*; D010450:Endopeptidases; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D016650:Fluorescein-5-isothiocyanate; D005455:Fluorescent Antibody Technique; D006735:Horseradish Peroxidase; D006825:Hybridomas; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D019535:Mycoplasma hominis / Q000276:immunology*; D010504:Periodic Acid; D013194:Staining and Labeling
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D007074:Immunoglobulin G; D010504:Periodic Acid; C009288:metaperiodate; D006735:Horseradish Peroxidase; D010450:Endopeptidases; D016650:Fluorescein-5-isothiocyanate