Differential prevention of experimental autoimmune encephalomyelitis with antigen-specific DNA vaccination.
We compared the potential therapeutic effect of vaccination with DNA constructs encoding two encephalitogenic proteins, PLP and MOG, on the outcome of subsequent sensitization of EAE induced in SJL/J and C57/B6 mice. Early sensitization for EAE (4 weeks after DNA vaccination) caused recipient animals to develop enhanced disease with DNA-encoding PLP but not with DNA-encoding MOG. Late sensitization (more than 10 weeks) resulted in an amelioration of EAE in animals vaccinated with both PLP and MOG DNA constructs. These results, confirming the DNA-mediated ameliorating effect on EAE, also indicate significant differences in the kinetics of development of EAE tolerance in response to vaccination with different DNA-encoding myelin antigens. Since PLP and MOG require different MHC presentation and induce different EAE models, the results point to potential differences in immune system requirements for efficient DNA-induced amelioration of the autoimmune response.
Walczak
Agata
A
Department of Neurology, Medical University of Lodz, 22 Kopcinskiego St., 90-153 Lodz, Poland.
Szymanska
Bozena
B
Selmaj
Krzysztof
K
D000818:Animals; D001324:Autoantigens / Q000276:immunology*; D019976:Cloning, Organism / Q000379:methods; D017931:DNA Primers / Q000276:immunology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000378:metabolism / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007114:Immunization / Q000379:methods*; D007279:Injections, Subcutaneous; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D007376:Interleukin-2 / Q000096:biosynthesis / Q000276:immunology; D015847:Interleukin-4 / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein / Q000096:biosynthesis / Q000276:immunology*; D018992:Myelin-Associated Glycoprotein / Q000096:biosynthesis / Q000276:immunology*; D063308:Myelin-Oligodendrocyte Glycoprotein; D010957:Plasmids / Q000008:administration & dosage; D016133:Polymerase Chain Reaction; D012313:RNA / Q000276:immunology; D013997:Time Factors; D019444:Vaccines, DNA / Q000008:administration & dosage*
D001324:Autoantigens; D017931:DNA Primers; D007376:Interleukin-2; C571327:Mog protein, mouse; D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D019444:Vaccines, DNA; D015847:Interleukin-4; D012313:RNA; D007371:Interferon-gamma