Syntaxin 1A and receptor for activated C kinase interact with the N-terminal region of human dopamine transporter.
The dopamine transporter (DAT) regulates the extent and duration of dopamine receptor activation through sodium-dependant reuptake of dopamine into presynaptic neurons, resulting in termination of dopaminergic neurotransmission. Using the yeast two-hybrid system, we have identified novel interactions between DAT, the SNARE protein syntaxin 1A, and the receptor for activated C kinases (RACK1). This association involves the intracellular N-terminal domain of human DAT (hDAT). Our data suggest that hDAT may exist as dimers or oligomers and that its protein-protein interactions with syntaxin 1A and RACK1 form functional regulatory complexes that may mediate DAT trafficking through modulation of hDAT phosphorylation by PKC.
Lee
Ki-Hwan
KH
Department of Biochemistry, College of Medicine, Hanyang University, Seoul, Korea.
Kim
Mi-Young
MY
Kim
Dong-Hwan
DH
Lee
Yong-Sung
YS
D000954:Antigens, Surface / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D003001:Cloning, Molecular; D050483:Dopamine Plasma Membrane Transport Proteins; D006801:Humans; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins / Q000737:chemistry* / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D050827:Syntaxin 1
D000954:Antigens, Surface; D050483:Dopamine Plasma Membrane Transport Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; C493214:SLC6A3 protein, human; C494212:STX1A protein, human; D050827:Syntaxin 1; D011493:Protein Kinase C