[The role of nitric oxide in the cytotoxic effect of macrophages].
OBJECTIVE
To explore the role of nitric oxide (NO) in cytotoxicity of macrophages.


METHODS
20 mice of Kunming species were divided into two groups. One group were injected subcutaneously with rhabdosarcoma cells S180 and the other were normal controls. The macrophages in peritoneal lavage fluid of two groups of mice were co-cultured with tumor cells K562. L-arginine(L-Arg) or NG nitro L-arginine methyleste(L-NAME) was added to the culture medium, and the cytotoxicity of macrophages was measured.


RESULTS
Macrophages from tumor bearing mice had stronger cytotoxicity than those from normal mice. L-Arg increased and L-NAME decreased the cytotoxicity of macrophages from tumor bearing mice, but both had no effect on the cytotoxicity of macrophages from normal mice.


CONCLUSIONS
NO is an effector molecule in cytotoxicity of activated macrophages.
Zhang
Jie
J
Department of Oncology, Shanghai Pulmonary Hospital, Shanghai 200433, China. liqiang_sh@lawyer.org.cn
Zhou
Cai-cun
CC
Su
Bo
B
D000818:Animals; D001120:Arginine / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D006801:Humans; D020014:K562 Cells; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000502:physiology*; D012510:Sarcoma 180 / Q000276:immunology
D009569:Nitric Oxide; D001120:Arginine; D019331:NG-Nitroarginine Methyl Ester