title: Large dense-core vesicle exocytosis in pancreatic beta-cells monitored by capacitance measurements.
abstract: This article discusses the currently used methodologies for monitoring exocytosis as changes in cell capacitance. Details are given on composition of solutions, experimental protocols, and how the observed responses can be interpreted physiologically. The concepts are illustrated by examples from our own work on insulin-releasing pancreatic beta-cells. Finally, we consider the feasibility of applying capacitance measurements to endocrine cells in intact pancreatic islets, where the cells are electrically coupled to each other.
authors.affiliation: Department of Physiology, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kanno
authors.forename: Xiasong
authors.initials: X
authors.lastname: Ma
authors.forename: Sebastian
authors.initials: S
authors.lastname: Barg
authors.forename: Lena
authors.initials: L
authors.lastname: Eliasson
authors.forename: Juris
authors.initials: J
authors.lastname: Galvanovskis
authors.forename: Sven
authors.initials: S
authors.lastname: Göpel
authors.forename: Max
authors.initials: M
authors.lastname: Larsson
authors.forename: Erik
authors.initials: E
authors.lastname: Renström
authors.forename: Patrik
authors.initials: P
authors.lastname: Rorsman
mesh_terms: D000818:Animals; D003594:Cytoplasmic Granules / Q000378:metabolism* / Q000648:ultrastructure; D005089:Exocytosis / Q000502:physiology*; D007515:Islets of Langerhans / Q000378:metabolism*; D013572:Synaptic Vesicles / Q000378:metabolism*