title: Transcriptional control of TFF3 (intestinal trefoil factor) via promoter binding sites for the nuclear factor kappaB and C/EBPbeta.
abstract: The acute phase response is strictly connected with modulation of gene expression. Transcriptional control of many genes is mediated by binding of diverse transcription factors to cis-acting DNA motifs in the respective promoter sequence. We now describe such regulatory elements for the TFF3 gene coding for a peptide involved in response to gut irritation. TNF-alpha stimulation, which induces NF-kappaB activation, evoked up to 10-fold reduction of TFF3 expression in the colon tumour cell line HT-29. Several consensus binding sites for members of the NF-kappaB transcription factor subunits are located in the 5'-flanking region of TFF3. Mutating these sites was aimed at discovering which one is responsible for NF-kappaB binding and thus regulation of TFF3 expression.
authors.affiliation: Division of Molecular Genetics, Institute of Anthropology & Human Genetics, University of Tübingen, Wilhelmstr 27, 72074, Germany.
authors.forename: Mirela
authors.initials: M
authors.lastname: Baus-Loncar
authors.forename: Ezzal-din
authors.initials: ED
authors.lastname: Al-azzeh
authors.forename: Hanna
authors.initials: H
authors.lastname: Romanska
authors.forename: El-Nasir
authors.initials: el-N
authors.lastname: Lalani
authors.forename: Gordon W H
authors.initials: GW
authors.lastname: Stamp
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Blin
authors.forename: Tuncay
authors.initials: T
authors.lastname: Kayademir
mesh_terms: D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics; D024202:Electrophoretic Mobility Shift Assay; D019073:HT29 Cells; D006801:Humans; D009077:Mucins / Q000235:genetics*; D009124:Muscle Proteins / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D016328:NF-kappa B / Q000235:genetics*; D010455:Peptides; D011401:Promoter Regions, Genetic; D051996:Transcription Factor RelA; D014157:Transcription Factors / Q000235:genetics*; D015533:Transcriptional Activation / Q000187:drug effects*; D000071165:Trefoil Factor-3; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D009077:Mucins; D009124:Muscle Proteins; D016328:NF-kappa B; D010455:Peptides; C082495:TFF3 protein, human; C439524:TNF protein, human; D051996:Transcription Factor RelA; D014157:Transcription Factors; D000071165:Trefoil Factor-3; D014409:Tumor Necrosis Factor-alpha