title: Enterocyte metabolism during early adaptation after extensive intestinal resection in a rat model.
abstract: OBJECTIVE
  A better knowledge of intestinal adaptation after resection is required to improve the nutritional support that is given to patients. The aim of this study was to understand the metabolic changes underlying early adaptation after massive intestinal resection.
  
  
  METHODS
  Rats were assigned to either 80% intestinal resection or transection. All animals received the same intragastric nutrition. On day 8, plasma glutamine turnover was measured. Substrate use was determined on isolated enterocytes that were incubated in the presence of D-[U-(14)C] glucose (2 mmol/L), L-[U-(14)C] glutamine (2 mmol/L), L-[U-(14)C] arginine (1 mmol/L), or L-[1-(14)C] ornithine (1 mmol/L).
  
  
  RESULTS
  Plasma glutamine turnover was similar in both groups. The rate of enterocyte glutamine use was significantly increased in the resection group, although the maximal glutaminase activity was unchanged. Glutathione generation was enhanced 3-fold in remnant intestine as compared with transected intestine (P <.05). L-ornithine decarboxylation was increased markedly in resected animals (P <.05), without any detectable change of maximal ornithine decarboxylase activity.
  
  
  CONCLUSIONS
  The early phase of intestinal adaptation after resection induces changes in enterocyte glutamine and ornithine metabolism that may be related, in part, to increased de novo polyamine synthesis. This observation suggests that a supplementation of artificial nutrition by nutrients that lead to the generation of trophic agents may be of potential interest.
authors.affiliation: Laboratoire de Nutrition et Sécurité Alimentaire, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
authors.forename: Hubert
authors.initials: H
authors.lastname: Lardy
authors.forename: Béatrice
authors.initials: B
authors.lastname: Mouillé
authors.forename: Muriel
authors.initials: M
authors.lastname: Thomas
authors.forename: Béatrice
authors.initials: B
authors.lastname: Darcy-Vrillon
authors.forename: Pierre
authors.initials: P
authors.lastname: Vaugelade
authors.forename: François
authors.initials: F
authors.lastname: Blachier
authors.forename: Françoise
authors.initials: F
authors.lastname: Bernard
authors.forename: Claire
authors.initials: C
authors.lastname: Cherbuy
authors.forename: Véronique
authors.initials: V
authors.lastname: Robert
authors.forename: Odile
authors.initials: O
authors.lastname: Corriol
authors.forename: Claude
authors.initials: C
authors.lastname: Ricour
authors.forename: Olivier
authors.initials: O
authors.lastname: Goulet
authors.forename: Pierre-Henri
authors.initials: PH
authors.lastname: Duée
authors.forename: Virginie
authors.initials: V
authors.lastname: Colomb
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D001120:Arginine / Q000378:metabolism; D001158:Arteries; D001835:Body Weight; D002469:Cell Separation; D002956:Citrulline / Q000096:biosynthesis; D003653:Decarboxylation; D020895:Enterocytes / Q000201:enzymology / Q000378:metabolism*; D005972:Glutaminase / Q000378:metabolism; D005973:Glutamine / Q000097:blood / Q000494:pharmacology; D005978:Glutathione / Q000096:biosynthesis; D007421:Intestine, Small / Q000473:pathology; D007422:Intestines / Q000503:physiopathology* / Q000601:surgery*; D008297:Male; D009952:Ornithine / Q000096:biosynthesis / Q000378:metabolism; D009955:Ornithine Decarboxylase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005973:Glutamine; D002956:Citrulline; D001120:Arginine; D009952:Ornithine; D005972:Glutaminase; D009955:Ornithine Decarboxylase; D005978:Glutathione