Ex vivo phenotype and frequency of influenza virus-specific CD4 memory T cells.
Recent advances in class II tetramer staining technology have allowed reliable direct ex vivo visualization of antigen-specific CD4 T cells. In order to define the frequency and phenotype of a prototype response to a nonpersistent pathogen, we have used such techniques to analyze influenza virus-specific memory CD4 T cells directly from blood. These responses are stably detectable ex vivo at low frequencies (range, 0.00012 to 0.0061% of CD4 T cells) and display a distinct "central memory" CD62L(+) phenotype.
Lucas
Michaela
M
Nuffield Department of Medicine, University of Oxford, United Kingdom.
Day
Cheryl L
CL
Wyer
Jessica R
JR
Cunliffe
Sharon L
SL
Loughry
Andrew
A
McMichael
Andrew J
AJ
Klenerman
Paul
P
D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D005802:Genes, MHC Class II; D006684:HLA-DR Antigens; D006801:Humans; D007156:Immunologic Memory; D019041:L-Selectin / Q000378:metabolism*; D009975:Orthomyxoviridae / Q000276:immunology*; D010641:Phenotype
D006684:HLA-DR Antigens; D019041:L-Selectin