Immunohistochemical localization of endothelial and inducible nitric oxide synthase within neurons of cattle with rabies.
The expression of constitutive endothelial nitric oxide synthase (eNOS) and inducible NOS (iNOS) in the brains of cattle with natural rabies was studied. Increased expression of eNOS was detected in neurons of the brain stem and Purkinje cells of cerebellum. By contrast, iNOS was diffusely localized in the cytoplasm of affected neurons, and some inflammatory cells were positive. eNOS and rabies antigen were co-localized in inclusion bodies (Negri bodies) in neurons. The specific localization of eNOS, but not iNOS, in the Negri bodies suggests that eNOS is involved in the formation of rabies virus inclusion bodies.
Shin
Taekyun
T
Department of Veterinary Medicine, Cheju National University, Jeju, Republic of Korea.
Weinstock
Daniel
D
Castro
Marlene D
MD
Hamir
Amir N
AN
Wampler
Thomas
T
Walter
Mark
M
Kim
Hyun Young
HY
Acland
Helen
H
D000818:Animals; D001921:Brain / Q000378:metabolism; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism*; D015870:Gene Expression; D007150:Immunohistochemistry; D007181:Inclusion Bodies, Viral / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; D011818:Rabies / Q000378:metabolism / Q000662:veterinary*
D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III