Characterization of bacterial diversity in pulque, a traditional Mexican alcoholic fermented beverage, as determined by 16S rDNA analysis.
The bacterial diversity in pulque, a traditional Mexican alcoholic fermented beverage, was studied in 16S rDNA clone libraries from three pulque samples. Sequenced clones identified as Lactobacillus acidophilus, Lactobacillus strain ASF360, L. kefir, L. acetotolerans, L. hilgardii, L. plantarum, Leuconostoc pseudomesenteroides, Microbacterium arborescens, Flavobacterium johnsoniae, Acetobacter pomorium, Gluconobacter oxydans, and Hafnia alvei, were detected for the first time in pulque. Identity of 16S rDNA sequenced clones showed that bacterial diversity present among pulque samples is dominated by Lactobacillus species (80.97%). Seventy-eight clones exhibited less than 95% of relatedness to NCBI database sequences, which may indicate the presence of new species in pulque samples.
Escalante
Adelfo
A
Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca, Morelos, 62250, Mexico.
Rodríguez
María Elena
ME
Martínez
Alfredo
A
López-Munguía
Agustín
A
Bolívar
Francisco
F
Gosset
Guillermo
G
D027882:Agave / Q000382:microbiology*; D000434:Alcoholic Beverages / Q000382:microbiology*; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis*; D017753:Ecosystem; D005285:Fermentation; D015723:Gene Library; D007778:Lactobacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D008800:Mexico; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D015183:Restriction Mapping
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S