[Effect of resveratrol alone and its combination with cyclosporin A on the immune function of human peripheral blood T lymphocytes].
OBJECTIVE
To investigate the effect of resveratrol (Res) alone and its combination with cyclosporin A (CsA) on the proliferation of human peripheral blood T lymphocytes (hPBTCs), transformation into lymphoblasts, as well as IL-2 and INF-gamma production.


METHODS
The proliferation of hPBTCs was detected by MTT colorimetry. The lymphocyte transformation rate was examined by morphologic method. The levels of IL-2 and INF-gamma in lymphocyte culture supernatant were detected by ELISA.


RESULTS
When the concentration of resveratrol was over 2.5 mg/L, especially 10 mg/L, the proliferation and transformation rate of hPBTCs decreased significantly (P<0.05). The levels of IL-2 and INF-gamma in lymphocyte culture supernatant reduced markedly (P<0.05), on condition that the concentration of resveratrol was over 5 mg/L. The combination of more than 2.5 mg/L of resveratrol with cyclosporine A had synergism.


CONCLUSIONS
Resveratrol can suppress notably the proliferation and transformation of human lymphocytes and the combination of resveratrol at a given concentration with cyclosporine A can enhance immune suppression.
Yu
Liang
L
Department of Hepatobiliary Surgery, First Hospital of Xi'an Jiaotong University, Xi'an 710061, China. Yuliang@163.com
Wu
Sheng-li
SL
Zhang
Mei
M
Pan
Cheng-en
CE
D016572:Cyclosporine / Q000494:pharmacology*; D004357:Drug Synergism; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000187:drug effects; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
D007376:Interleukin-2; D013267:Stilbenes; D007371:Interferon-gamma; D016572:Cyclosporine; D000077185:Resveratrol