title: Heterogeneous nuclear ribonucleoprotein A2/B1 identified as an autoantigen in autoimmune hepatitis by proteome analysis.
abstract: Our aim was to characterize antinuclear antibody (ANA) targets in type 1 autoimmune hepatitis (AIH1) using a proteomic tool. ANA-positive sera from 29 patients with AIH1 and 14 negative controls were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The principal antigens identified were a 30 kDa triplet band which was recognized by 79% of patient sera and 14% of control sera, and a 36 kDa antigen which was recognized by 52% and 14% of sera, respectively. The latter antigen was studied in more detail using two-dimensional electrophoresis (2-DE) and mass spectrometry (MS). Its identification corresponded to heterogeneous nuclear ribonucleoprotein A2/B1. This paper presents the first description of this protein as an antigen in AIH1.
authors.affiliation: Service d'Immunologie et Hématologie biologiques, Hôpital Saint-Antoine, Paris, France.
authors.forename: Stèphanie
authors.initials: S
authors.lastname: Huguet
authors.forename: Valèrie
authors.initials: V
authors.lastname: Labas
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Duclos-Vallee
authors.forename: Arnaud
authors.initials: A
authors.lastname: Bruneel
authors.forename: Joëlle
authors.initials: J
authors.lastname: Vinh
authors.forename: Didier
authors.initials: D
authors.lastname: Samuel
authors.forename: Catherine
authors.initials: C
authors.lastname: Johanet
authors.forename: Eric
authors.initials: E
authors.lastname: Ballot
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000974:Antibodies, Antinuclear / Q000032:analysis; D001324:Autoantigens / Q000276:immunology*; D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005602:France; D019693:Hepatitis, Autoimmune / Q000276:immunology*; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D006801:Humans; D015151:Immunoblotting; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D008970:Molecular Weight; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D016622:Silver Staining; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000974:Antibodies, Antinuclear; D001324:Autoantigens; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D020543:Proteome