Characterization of endothelial internalization and targeting of antibody-enzyme conjugates in cell cultures and in laboratory animals.
Streptavidin-biotin conjugates of enzymes with carrier antibodies provide a versatile means for targeting selected cellular populations in cell cultures and in vivo. Both specific delivery to cells and proper subcellular addressing of enzyme cargoes are important parameters of targeting. This chapter describes methodologies for evaluating the binding and internalization of labeled conjugates directed to endothelial surface adhesion molecules in cell cultures using anti-intercellular adhesion molecule/catalase or antiplatelet endothelial cell adhesion molecule/catalase conjugates as examples. It also describes protocols for characterization of biodistribution and pulmonary targeting of radiolabeled conjugates in rats using anti-intercellular adhesion molecule/tPA conjugates as an example. The experimental procedures, results, and notes provided may help in investigations of vascular immunotargeting of reporter, experimental, diagnostic, or therapeutic enzymes to endothelial and, perhaps, other cell types, both in vitro and in vivo.
Muro
Silvia
S
Institute for Environmental Medicine, University of Pennsylvania Medical Center, Philadelphia, USA.
Muzykantov
Vladimir R
VR
Murciano
Juan-Carlos
JC
D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D016503:Drug Delivery Systems; D004727:Endothelium / Q000378:metabolism*; D004798:Enzymes / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D018796:Immunoconjugates / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D008168:Lung / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009928:Organ Specificity; D016719:Radioimmunodetection / Q000379:methods; D051381:Rats
D000906:Antibodies; D004798:Enzymes; D018796:Immunoconjugates