title: Evidence for existence of oligoclonal tumor-infiltrating lymphocytes and predominant production of T helper 1/T cytotoxic 1 type cytokines in gastric and colorectal tumors.
abstract: Tumor-infiltrating lymphocytes (TIL) play a central role in cellular immunity against tumor. We have revealed the characteristics of TILs in terms of T-cell receptor (TCR) repertoire, T-cell clonality, and cytokine production. TCR repertoire analyses and CDR3 size spectratyping were performed using peripheral blood mononuclear cells (PBMCs) and tissue specimens of gastric or colorectal cancers surgically resected from 11 patients. The cytokine expression was measured by real-time quantitative polymerase chain reaction. TCR repertoires were similar among multiple tissue specimens from different sites of the same tumor. Similar peak patterns of CDR3 size spectratyping were observed among these tumor specimens, but not in normal tissues or PBMCs. In addition, identical peaks were detected in multiple specimens of the same tumor. The ratio of the levels of IFN-gamma to that of IL-4 is significantly higher for tumor lesions compared with PBMCs. These results suggested that a limited number of TILs locally expand in response to tumor antigens exiting within gastric or colorectal cancers and local predominant production of the T helper 1/T cytotoxic 1 type cytokine may affect the anti-tumor immune response of TILs.
authors.affiliation: Division of Immunology and Embryology, Department of Cell Biology, Tohoku University School of Medicine, Sendai 980-8575, Japan. matsu@immem.med.tohoku.ac.jp
authors.forename: Takaji
authors.initials: T
authors.lastname: Matsutani
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Shiiba
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yoshioka
authors.forename: Yuji
authors.initials: Y
authors.lastname: Tsuruta
authors.forename: Ryuji
authors.initials: R
authors.lastname: Suzuki
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ochi
authors.forename: Tsunetoshi
authors.initials: T
authors.lastname: Itoh
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Musha
authors.forename: Takayuki
authors.initials: T
authors.lastname: Mizoi
authors.forename: Iwao
authors.initials: I
authors.lastname: Sasaki
mesh_terms: D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D003110:Colonic Neoplasms / Q000276:immunology / Q000473:pathology / Q000601:surgery; D015179:Colorectal Neoplasms / Q000276:immunology* / Q000473:pathology / Q000601:surgery; D022801:Complementarity Determining Regions / Q000235:genetics; D016207:Cytokines / Q000032:analysis / Q000235:genetics*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010446:Peptide Fragments; D016133:Polymerase Chain Reaction; D012004:Rectal Neoplasms / Q000276:immunology / Q000473:pathology / Q000601:surgery; D013274:Stomach Neoplasms / Q000276:immunology* / Q000473:pathology / Q000601:surgery; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000473:pathology
chemical_list: D022801:Complementarity Determining Regions; D016207:Cytokines; D010446:Peptide Fragments; D015847:Interleukin-4; D007371:Interferon-gamma