Detection and analysis of tumor fluorescence using a two-photon optical fiber probe.
The utility of a two-photon optical fiber fluorescence probe (TPOFF) for sensing and quantifying tumor fluorescent signals was tested in vivo. Xenograft tumors were developed in athymic mice using MCA207 cells expressing green fluorescent protein (GFP). The TPOFF probe was able to detect ex vivo fluorescence from excised tumors containing as little as 0.3% GFP-expressing cells. TPOFF results were similar to both flow-cytometric analysis of tumor cells after isolation and suspension, and fluorescence determined by microscope images of cryosectioned tumors. TPOFF was then used to measure GFP fluorescence from tumors in live mice. The fiber probe detected fluorescently-labeled Herceptin antibody targeted to HER2-expressing tumors in severe combined immunodeficient mice. Dendrimer nanoparticles targeted by folic acid and having 6-TAMRA as a fluorescent probe were also used to label KB cell tumors in vivo. The fiber probe documented a fourfold increase in tumor fluorescence in animals that received the targeted dendrimer. These results suggest TPOFF can be used as a minimally invasive system for identifying tumor markers and monitoring drug therapy.
Thomas
Thommey P
TP
Center for Biologic Nanotechnology, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Myaing
Mon Thiri
MT
Ye
Jing Yong
JY
Candido
Kimberly
K
Kotlyar
Alina
A
Beals
James
J
Cao
Peter
P
Keszler
Balazs
B
Patri
Anil K
AK
Norris
Theodore B
TB
Baker
James R
JR
D000818:Animals; D003001:Cloning, Molecular; D016616:Cryoultramicrotomy; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry*; D005492:Folic Acid / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D017785:Photons; D014183:Transplantation, Heterologous / Q000473:pathology*; D014407:Tumor Cells, Cultured
D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D005492:Folic Acid