[Changes of intestinal mucosal lymphocyte homing in rats with multiple organ dysfunction syndromes].
OBJECTIVE
To observe the changes of intestinal mucosal lymphocyte homing in rats with multiple organ dysfunction syndromes (MODS) and study the role of intestinal mucosal lymphocyte play in MODS.


METHODS
A rat model of MODS was reproduced by occluding the superior mesenteric artery for 45 minutes followed by reperfusion for 6 hours. From the fifth hour after reperfusion, intestinal lymph of rats (MODS group 1, n=10) was collected for 1 hour. Intestinal lymph was also collected from rats of control group 1 (n=6). The population of lymphocytes and the percentage of T and B cells were measured in both groups. From the third hour after reperfusion, intestinal lymph of rats (MODS group 2, n=6) was collected for 2 hours. Then, intestinal lymphocytes were labeled with (51)Cr and were infused into blood circulation again at the sixth hour of reperfusion. Various organs were taken out 1 hour later for measurement of (51)Cr-lymphocytes distribution in organs by gamma-counter. Intestinal lymph of control rats (group 2, n=6) was collected. Labeled lymphocytes were also infused into blood circulation of rats again.


RESULTS
The number of lymphocytes from intestinal mucosa migrated into blood circulation was decreased, the counts were (0.28+/-0.15)X10(7)/h and (2.69+/-0.61)X10(7)/h respectively, while 51 Cr-intestinal lymphocytes homed to intestinal mucosa were increased in MODS induced by intestine ischemia/reperfusion. At the same time, the levels of endotoxin and tumor necrosis factor-alpha (TNF-alpha) in intestinal lymph were also elevated.


CONCLUSIONS
Increased homing of intestinal mucosal lymphocyte is involved in MODS of rats.
Yang
Hui
H
Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.
Tang
Cheng-Wei
CW
D000818:Animals; D004195:Disease Models, Animal; D041981:Gastrointestinal Tract / Q000098:blood supply / Q000473:pathology / Q000503:physiopathology; D007413:Intestinal Mucosa / Q000098:blood supply / Q000473:pathology / Q000503:physiopathology*; D007511:Ischemia / Q000378:metabolism / Q000503:physiopathology; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D009102:Multiple Organ Failure / Q000473:pathology / Q000503:physiopathology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D014409:Tumor Necrosis Factor-alpha