title: Nuclear sequestration of cellular chaperone and proteasomal machinery during herpes simplex virus type 1 infection.
abstract: Herpes simplex virus type 1 (HSV-1) encodes a portal protein that forms a large oligomeric structure believed to provide the conduit for DNA entry and exit from the capsid. Chaperone proteins often facilitate the folding and multimerization of such complex structures. In this report, we show that cellular chaperone proteins, components of the 26S proteasome, and ubiquitin-conjugated proteins are sequestered in discrete foci in the nucleus of the infected cell. The immediate-early viral protein ICP0 was shown to be necessary to establish these foci at early times during infection and sufficient to redistribute chaperone molecules in transfected cells. Furthermore, we found that not only is the portal protein, UL6, localized to these sites during infection, but it is also a substrate for ubiquitin modification. Our results suggest that HSV-1 has evolved an elegant mechanism for facilitating protein quality control at specialized foci within the nucleus.
authors.affiliation: Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, 06030, USA.
authors.forename: April D
authors.initials: AD
authors.lastname: Burch
authors.forename: Sandra K
authors.initials: SK
authors.lastname: Weller
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D002522:Chlorocebus aethiops; D050883:HSC70 Heat-Shock Proteins; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism*; D018259:Herpesvirus 1, Human / Q000472:pathogenicity*; D006801:Humans; D017874:Immediate-Early Proteins / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex; D025801:Ubiquitin / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases; D014709:Vero Cells; D014764:Viral Proteins
chemical_list: D036022:Capsid Proteins; D050883:HSC70 Heat-Shock Proteins; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C494371:HSPA8 protein, human; D006360:Heat-Shock Proteins; D017874:Immediate-Early Proteins; D025801:Ubiquitin; D014764:Viral Proteins; C069185:DNA cleavage and packaging proteins, Herpesvirus; D044767:Ubiquitin-Protein Ligases; C051804:Vmw110 protein, Human herpesvirus 1; D010447:Peptide Hydrolases; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease