Effects of Helicobacter pylori water extract on expression of endothelial adhesion molecules.
The present study investigated whether Helicobacter pylori water extract induces the upregulation of intercellular adhesion molecule-1, vascular cell adhesion molecule-1 and E-selectin on human umbilical vein endothelial cells, using an ELISA. The nature of the substances mediating this upregulation was also analyzed. H pylori water extract derived from type strain (NCTC 11637) significantly upregulated intercellular adhesion molecule-1, vascular cell adhesion molecule-1 and E-selectin to the same extent as interleukin-1. Treatments with extracts from clinical strains showed no significant increases in expression of these adhesion molecules. In a fractionation study, approximately 7 kDa fraction showed peak activity. This activity was tolerant to heating and trypsin digestion. These results indicate that H pylori water extract contains water-soluble, low-molecular, nonprotein substances which induce upregulation of adhesion molecules on human umbilical vein endothelial cells, suggesting that products of H pylori may elicit gastric mucosal inflammation by promoting expression of endothelial adhesion molecules.
Yoshida
Norimasa
N
Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Japan. nyoshida@koto.kpu-m.ac.jp
Kassai
Kyoichi
K
Murase
Hironobu
H
Tanaka
Yukiko
Y
Kokura
Satoshi
S
Ichikawa
Hiroshi
H
Naito
Yuji
Y
Okanoue
Takeshi
T
Yoshikawa
Toshikazu
T
D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000187:drug effects; D002850:Chromatography, Gel; D019040:E-Selectin / Q000032:analysis / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D016480:Helicobacter pylori / Q000737:chemistry*; D006358:Hot Temperature; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000187:drug effects; D007375:Interleukin-1 / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000032:analysis; D008970:Molecular Weight; D012995:Solubility; D014357:Trypsin / Q000187:drug effects; D014471:Umbilical Veins / Q000166:cytology; D015854:Up-Regulation; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis / Q000187:drug effects; D014867:Water
D015815:Cell Adhesion Molecules; D019040:E-Selectin; D007375:Interleukin-1; D008070:Lipopolysaccharides; D019010:Vascular Cell Adhesion Molecule-1; D014867:Water; D018799:Intercellular Adhesion Molecule-1; D014357:Trypsin