Cloning of IP15, a pancreatitis-induced gene whose expression inhibits cell growth.
We describe the cloning and expression of the mouse gene interferon-inducible-protein 15 (IP15), whose activation is related to the acute phase of experimental pancreatitis. Analysis of its structure indicates that it encodes a putative transmembrane protein of 137 amino acids. This gene contains a predicted IFN-stimulable-response element. In vivo studies showed that IP15 is strongly activated in pancreas early during caerulein-induced pancreatitis. In situ hybridization of IP15 mRNA showed that its expression is restricted to acinar cells. IP15 was also induced in pancreas under systemic-lipopolysaccharide treatment and in intestine under Salmonella infection. In vitro studies using NIH3T3 fibroblasts showed that IP15 is induced by IFN-alpha. Growth rate was significantly lower in cells transfected with pcDNA4/IP15 plasmid. In addition, cells expressing IP15 showed less capacity to develop colonies after antibiotic selection. In conclusion, we identified a new interferon-inducible gene that is activated early in pancreas with pancreatitis and whose expression inhibits cell growth.
Ropolo
Alejandro
A
Department of Physiology, School of Medicine, Buenos Aires University, Buenos Aires, Argentina.
Tomasini
Richard
R
Grasso
Daniel
D
Dusetti
Nelson J
NJ
Cerquetti
María C
MC
Iovanna
Juan L
JL
Vaccaro
María I
MI
D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division / Q000502:physiology*; D002460:Cell Line; D002108:Ceruletide / Q000633:toxicity; D003001:Cloning, Molecular; D005260:Female; D005333:Fetus / Q000502:physiology; D006801:Humans; D017403:In Situ Hybridization; D007372:Interferons / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010179:Pancreas / Q000166:cytology / Q000502:physiology; D010195:Pancreatitis / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D020218:Response Elements; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
C488102:IP15 protein, mouse; D008565:Membrane Proteins; D012333:RNA, Messenger; D002108:Ceruletide; D007372:Interferons