[The change of the erythrocyte chemokine receptor binding activity in the shock stage of burn rats].
OBJECTIVE
To investigate the change of the erythrocyte chemokine receptor(ECKR) binding activity in the shock stage of burn rats.


METHODS
SD rats were randomly divided into two group, burn and control groups. In the burn group rats, 30% total body surface area (TBSA)were scalded to III degree. The binding activity of rat ECKR in the shock stage was detected by ELISA using IL-8 as ligand at various time points (0.5, 2, 4, 8, 16, 24 and 48 hours) after burn.


RESULTS
Compared with control group, the binding activity of rat ECKR declined significantly half an hour after burn and maintained at low level for 48 hours (P<0.01). The comparison of the binding activity of rat ECKR at various time points after burn indicated that the binding activity declined gradually from 2 hours (P<0.01-0.05), reached lowest value 24 hrs, and then rose significantly 48 hrs after burn (P<0.01).


CONCLUSIONS
The ECKR binding activity declined significantly after burn, suggesting erythrocytes may participate in the regulation of chemokines and play some role in the inflammation.
Chen
Xiao-dong
XD
Provincial Burns Institute, Union Hospital, Fujian Medical University, Fuzhou 350001, China. fzxdchen@sina.com
Wang
Shun-bin
SB
Wu
Bo-yu
BY
Jiang
Qiong
Q
D000818:Animals; D002056:Burns / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D016209:Interleukin-8 / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019707:Receptors, Chemokine / Q000378:metabolism*; D012774:Shock, Traumatic / Q000276:immunology*; D013997:Time Factors
D016209:Interleukin-8; D019707:Receptors, Chemokine