Reduced expression of the let-7 microRNAs in human lung cancers in association with shortened postoperative survival.
In this study, we report for the first time reduced expression of the let-7 microRNA in human lung cancers. Interestingly, 143 lung cancer cases that had undergone potentially curative resection could be classified into two major groups according to let-7 expression in unsupervised hierarchical analysis, showing significantly shorter survival after potentially curative resection in cases with reduced let-7 expression (P = 0.0003). Multivariate COX regression analysis showed this prognostic impact to be independent of disease stage (hazard ratio = 2.17; P = 0.009). In addition, overexpression of let-7 in A549 lung adenocarcinoma cell line inhibited lung cancer cell growth in vitro. This study represents the first report of reduced expression of let-7 and the potential clinical and biological effects of such a microRNA alteration.
Takamizawa
Junichi
J
Division of Molecular Oncology, Aichi Cancer Center Research Institute, Nagoya, Japan. tak@aichi-cc.jp
Konishi
Hiroyuki
H
Yanagisawa
Kiyoshi
K
Tomida
Shuta
S
Osada
Hirotaka
H
Endoh
Hideki
H
Harano
Tomoko
T
Yatabe
Yasushi
Y
Nagino
Masato
M
Nimura
Yuji
Y
Mitsudomi
Tetsuya
T
Takahashi
Takashi
T
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D002455:Cell Division / Q000235:genetics; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D014176:Protein Biosynthesis; D011506:Proteins
D035683:MicroRNAs; D011506:Proteins; C487931:mirnlet7 microRNA, human