title: Quantification of solid cell material by detection of membrane-associated proteins and peptidoglycan.
abstract: Quantification of solid cell material (cell debris) is necessary for the optimisation of the efficiency of bioseparations. Cell debris can be quantified by detection of a component present in the cell wall that can act as a marker for cell debris. Membrane-associated proteins have previously been used as a marker for cell debris. This marker was quantified by SDS-PAGE with densiometry. In this paper cell debris quantification methods are presented that are faster and more accurate, i.e. membrane-associated protein quantification with the Protein 50 Labchip of Agilent Technologies, or that make use of peptidoglycan as marker for cell debris, i.e. a spectrophotometric muramic acid assay.
authors.affiliation: Department of Biotechnology, Delft University of Technology Julianalaan 67, 2628 BC Delft, The Netherlands.
authors.forename: P
authors.initials: P
authors.lastname: van Hee
authors.forename: A P J
authors.initials: AP
authors.lastname: Middelberg
authors.forename: R G J M
authors.initials: RG
authors.lastname: van der Lans
authors.forename: L A M
authors.initials: LA
authors.lastname: van der Wielen
mesh_terms: D002849:Chromatography, Gas; D004591:Electrophoresis, Polyacrylamide Gel; D005285:Fermentation; D008565:Membrane Proteins / Q000032:analysis*; D010457:Peptidoglycan / Q000032:analysis*
chemical_list: D008565:Membrane Proteins; D010457:Peptidoglycan