Impact of dextran sulfate sodium load on the severity of inflammation in experimental colitis.
In dextran sulfate sodium (DSS)-induced inflammatory bowel disease in mice the relationship between the amount of ingested DSS and the severity of colitis has not been systematically investigated. We examined whether (1) the severity of colitis is DSS load-dependent, and (2) there is a critical DSS load required to reliably induce colitis. DSS load was calculated as: (drinking volume (ml) x [DSS (g)/100 ml])/body weight (g). A minimum DSS load > or = 30 mg/g body weight over 7 days resulted in a significantly elevated colonic myeloperoxidase (MPO) activity, compared to mice receiving less DSS and controls (P < 0.05). Histomorphologic data correlated with MPO activity and revealed significantly higher damage scores once the DSS load was > or = 30 mg/g body weight. Our findings demonstrate the importance of monitoring DSS load in this model of experimental colitis.
Vowinkel
Thorsten
T
Department of Molecular and Cellular Physiology, LSU Health Sciences Center, 1501 Kings Highway, Shreveport, Louisiana 71130-3932, USA.
Kalogeris
Theodore J
TJ
Mori
Mikiji
M
Krieglstein
Christian F
CF
Granger
D Neil
DN
D000818:Animals; D001707:Biopsy, Needle; D003092:Colitis / Q000139:chemically induced* / Q000473:pathology*; D003106:Colon / Q000187:drug effects* / Q000473:pathology; D016264:Dextran Sulfate / Q000009:adverse effects* / Q000494:pharmacology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011336:Probability; D011897:Random Allocation; D012016:Reference Values; D012044:Regression Analysis; D012307:Risk Factors; D013045:Species Specificity
D016264:Dextran Sulfate