Effect of alpha-tocopherol and simvastatin on male fertility in hypercholesterolemic rats.
The effect of alpha-tocopherol, simvastatin and both on male fertility in hypercholesterolemic rats was studied. Induction of hypercholesterolemia was done by feeding rats on a diet containing 1% cholesterol for 30 days. Hypercholesterolemic rats were orally given alpha-tocopherol (3 mg kg(-1) BW) or simvastatin (1 mg kg(-1) BW) or both for 65 days. Fertility index, serum testosterone level, sex organs weight, semen analysis and histopathological examination of testes, seminal vesicles and prostate glands were the parameters used to evaluate the reproductive efficiency of rats. In hypercholesterolemic rats (control +ve), there was a marked decrease in fertility index, testicular weight, sperm cell count, and percentages of sperm motility and viability associated with a significant increase in sperm cell abnormalities. Oral administration of either alpha-tocopherol or simvastatin to hypercholesterolemic rats for 65 days significantly improved the fertility index, testicular weight and semen quality. Concomitant administration of alpha-tocopherol and simvastatin to hypercholesterolemic rats markedly increased fertility index and sperm motility and viability associated with a significant reduction of sperm cell abnormalities. Histopathological examination revealed that testes of hypercholesterolemic rats (control +ve) had degenerated, non-functioning and atrophied seminiferous tubules associated with arrest of spermatogenesis. Oral administration of alpha-tocopherol and simvastatin concomitantly to hypercholesterolemic rats resulted in active mature and full functioning seminiferous tubules. In conclusion, concomitant administration of alpha-tocopherol and simvastatin to hypercholesterolemic male rats improved their reproductive efficiency and produced additional protection against reduced fertility induced by hypercholesterolemia.
Shalaby
M A
MA
Pharmacology Department, Faculty of Veterinary Medicine, Cairo University, P.O. Box 12211, Giza, Egypt.
el-Zorba
H Y
HY
Kamel
Gehan M
GM
D000284:Administration, Oral; D000818:Animals; D002214:Capsules; D002791:Cholesterol, Dietary / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology; D004032:Diet; D004334:Drug Administration Schedule; D004359:Drug Therapy, Combination; D005260:Female; D005780:Gelatin; D006937:Hypercholesterolemia / Q000139:chemically induced / Q000150:complications / Q000188:drug therapy*; D007248:Infertility, Male / Q000150:complications / Q000188:drug therapy*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011467:Prostate / Q000187:drug effects; D051381:Rats; D012098:Reproduction / Q000187:drug effects / Q000502:physiology; D012661:Semen / Q000737:chemistry / Q000187:drug effects; D012671:Seminiferous Tubules / Q000187:drug effects / Q000473:pathology; D019821:Simvastatin / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D013076:Sperm Count / Q000379:methods; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000002:abnormalities / Q000187:drug effects; D013737:Testis / Q000737:chemistry / Q000187:drug effects / Q000648:ultrastructure; D013739:Testosterone / Q000097:blood; D013997:Time Factors; D024502:alpha-Tocopherol / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
D002214:Capsules; D002791:Cholesterol, Dietary; D013739:Testosterone; D005780:Gelatin; D019821:Simvastatin; D024502:alpha-Tocopherol