title: Green tea polyphenols prevent ultraviolet light-induced oxidative damage and matrix metalloproteinases expression in mouse skin.
abstract: Chronic exposure of solar ultraviolet (UV) light to human skin results in photoaging. UV-induced oxidative damage and induction of matrix metalloproteinases (MMP) have been implicated in this process. Because polyphenols from green tea (GTP) prevent other cutaneous adverse effects of UV radiation we hypothesized that UV irradiation-induced oxidative damage and induction of MMP might be prevented in vivo in mouse skin by oral administration of GTP. GTP was administered in drinking water (0.2%, wt/vol) to SKH-1 hairless mice, which were then exposed to multiple doses of UVB (90 mJ per cm2, for 2 mo on alternate days) following in vivo photoaging animal protocol. Treatment of GTP resulted in inhibition of UVB-induced protein oxidation in vivo in mouse skin, a hallmark of photoaging, when analyzed biochemically, by immunoblotting, and immunohistochemistry. GTP treatment also inhibited UVB-induced protein oxidation in vitro in human skin fibroblast HS68 cells, which supports in vivo observations. Moreover, oral administration of GTP also resulted in inhibition of UVB-induced expression of matrix degrading MMP, such as MMP-2 (67%), MMP-3 (63%), MMP-7 (62%), and MMP-9 (60%) in hairless mouse skin. These data suggest that GTP as a dietary supplement could be useful to attenuate solar UVB light-induced premature skin aging.
authors.affiliation: Department of Dermatology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA.
authors.forename: Praveen K
authors.initials: PK
authors.lastname: Vayalil
authors.forename: Anshu
authors.initials: A
authors.lastname: Mittal
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Hara
authors.forename: Craig A
authors.initials: CA
authors.lastname: Elmets
authors.forename: Santosh K
authors.initials: SK
authors.lastname: Katiyar
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002478:Cells, Cultured; D024141:Collagen Type IV / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology / Q000528:radiation effects; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D051379:Mice; D008812:Mice, Hairless; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects*; D010636:Phenols / Q000494:pharmacology*; D059808:Polyphenols; D012867:Skin / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D013047:Specific Pathogen-Free Organisms; D013662:Tea / Q000737:chemistry*; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D024141:Collagen Type IV; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D013662:Tea; D020782:Matrix Metalloproteinases; D019278:Matrix Metalloproteinase 3; D020783:Matrix Metalloproteinase 7; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9