title: Modulation of sciatic nerve expression of class 3 semaphorins by nerve injury.
abstract: Beginning with the unexpected finding by cDNA array analysis that neuropilin-2 is induced in sciatic nerve distal to a transection, we document, for the first time, up-regulation in the axotomized adult peripheral nervous system of class 3 semaphorins and their receptors, which are known to play prominent roles in axonal guidance during neural development. Previously, we described the use of cDNA arrays to screen for novel peripheral nervous system axotomy-induced candidate neurotrophic proteins. A novel finding of that prior study was substantial induction of neuropilin 2 (NP2) mRNA in the axotomized nerve segments. Following up on that initial observation, we have now used real-time quantitative reverse transcription-polymerase chain reaction to demonstrate induction of genes encoding neuropilin 1 (NP1), which, like NP2, serves as a coreceptor for members of the class 3 semaphorin family of axonal guidance molecules and of five of the six known class 3 semaphorins (Sema3A, Sema3B, Sema3C, Sema3E, and Sema3F, but not Sema3D) in crushed or transected sciatic nerves.
authors.affiliation: Division of Neurology, Joseph Stokes, Jr. Research Institute, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA.
authors.forename: Jahan
authors.initials: J
authors.lastname: Ara
authors.forename: Peter
authors.initials: P
authors.lastname: Bannerman
authors.forename: Ashleigh
authors.initials: A
authors.lastname: Hahn
authors.forename: Sylvia
authors.initials: S
authors.lastname: Ramirez
authors.forename: David
authors.initials: D
authors.lastname: Pleasure
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005786:Gene Expression Regulation / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D009409:Nerve Crush; D039921:Neuropilins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012584:Sciatic Nerve / Q000293:injuries* / Q000503:physiopathology*; D039961:Semaphorins / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D017931:DNA Primers; D039921:Neuropilins; D012333:RNA, Messenger; D039961:Semaphorins