[The cytokine secretion of peripheral blood mononucleocytes from patients infected with HCV].
OBJECTIVE
To detect the levels of cytokines secreted by PBMC from patients infected with HCV after culture for 72 hours in-vitro, so as to reflect the immune state of the HCV-infected patients.


METHODS
The levels of cytokines in the culture supernatant of PBMCs were detected by ELISA.


RESULTS
(1)Compared with the cytokine level of normal control, the levels of IFN-gamma, TNF-alpha, and IL-10 of HCV patients notably increased while IL-2, IL-4, and IL-12 were not detected in the culture supernatant of PBMCs from both normal control and HCV patients after culture for 72 hours. (2)There was no statistic difference in secretion of cytokines among the patients with mild, and moderate chronic hepatitis, and compensated or uncompensated cirrhosis.


CONCLUSIONS
(1)PMBCs from the HCV-infected patients tend to secret Th2 type cytokines. (2)Lack of the IL-2 secretion from PBMCs of HCV patients may account for the immune escape of HCV.
Dong
Yi
Y
Department of Immunology, Institute of Infectious Diseases, 302th Hospital of PLA, Beijing 100039, China. yuncheng21@yahoo.com
Zhang
Hong-fei
HF
Chen
Hao
H
Yang
Xiao-jin
XJ
Li
Jing
J
Shu
Cui-li
CL
Cheng
Yun
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D002648:Child; D016207:Cytokines / Q000097:blood*; D005260:Female; D019698:Hepatitis C, Chronic / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D016753:Interleukin-10 / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000276:immunology; D008297:Male; D008875:Middle Aged; D018418:Th2 Cells / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma