title: Versatile analysis of multiple macromolecular interactions by SPR imaging: application to p53 and DNA interaction.
abstract: The greatest challenge in the postgenomic era is the description of proteome interactions, such as protein-protein or protein-DNA interactions. Surface plasmon resonance (SPR) is an optical technique in which binding of an analyte to the surface changes the refractive index at the surface/solution interface. Molecular interactions are analysed in real time without a labeling step. Currently, the limit to SPR imaging is the small number of reactions that can be simultaneously analysed. Using a novel grafting technology and a new imaging system, we increased the throughput of SPR imaging. The interaction between p53 and DNA was chosen as a paradigm for validation of this assay. Using a tagged DNA methodology, we simultaneously targeted multiple DNA sequences on a single chip. The interaction between p53 and these DNA sequences was monitored by SPR imaging. Qualitative and quantitative analysis provides results similar to those obtained with conventional technologies.
authors.affiliation: Laboratoire Charles Fabry de l'Institut d'Optique (LCFIO), Centre National de la Recherche Scientifique CNRS UMR 8501, Bâtiment 503, Université Paris XI, 91403 Orsay, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Maillart
authors.forename: Karen
authors.initials: K
authors.lastname: Brengel-Pesce
authors.forename: Delphine
authors.initials: D
authors.lastname: Capela
authors.forename: André
authors.initials: A
authors.lastname: Roget
authors.forename: Thierry
authors.initials: T
authors.lastname: Livache
authors.forename: Michael
authors.initials: M
authors.lastname: Canva
authors.forename: Yves
authors.initials: Y
authors.lastname: Levy
authors.forename: Thierry
authors.initials: T
authors.lastname: Soussi
mesh_terms: D004247:DNA / Q000378:metabolism*; D006801:Humans; D020349:Surface Plasmon Resonance / Q000379:methods*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D016159:Tumor Suppressor Protein p53; D004247:DNA