NaoXinQing, an anti-stroke herbal medicine, reduces hydrogen peroxide-induced injury in NG108-15 cells.
NaoXinQing (NXQ) is a patented and approved drug of Traditional Chinese Medicine that has been used for years for the treatment of syndrome of apoplexy, but the underlying mechanism remains unclear. The present study is designed to investigate the effects of NXQ on hydrogen peroxide (H(2)O(2))-induced cell damage in NG108-15 cells. Exposure to H(2)O(2) induces apoptosis-like cell injury. Preincubation of cells with NXQ alleviated H(2)O(2)-induced cell injury and apoptosis. This herb medicine also improves redox imbalance in cells under the exposure of H(2)O(2) as indicated by the attenuation in the reduction of activities of intracellular endogenous antioxidants, glutathione and glutathione peroxidase as well as catalase, and by the decrease in the leak of lactate dehydrogenase and the accumulation of malondialdehyde. These results indicate that NXQ significantly protects NG108-15 cells against H(2)O(2) challenge by improving redox imbalance and inhibiting apoptosis, which might represent mechanisms underlying its potential usage in the prevention and treatment of syndrome of apoplexy.
Bei
WeiJian
W
Department of Biochemistry, College of Life Sciences, Sun Yatsen (Zhongshan) University, 135 W. Xingang Road, Guangzhou, Guangdong, 510275, PR China.
Peng
Wenlie
W
Ma
Yan
Y
Xu
Anlong
A
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D005434:Flow Cytometry / Q000379:methods; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006836:Hydro-Lyases / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D008516:Medicine, Chinese Traditional; D051379:Mice; D009447:Neuroblastoma; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D020521:Stroke / Q000188:drug therapy / Q000473:pathology; D013778:Tetrazolium Salts; D013844:Thiazoles; D014407:Tumor Cells, Cultured
D004365:Drugs, Chinese Herbal; D013778:Tetrazolium Salts; D013844:Thiazoles; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D006836:Hydro-Lyases; C045581:lactate dehydratase; C022616:thiazolyl blue; D005978:Glutathione