Purification of plastocyanin and cytochrome c6 from plants, green algae, and cyanobacteria.
Plastocyanin and cytochrome c6 are widely distributed over the oxygen-evolving photosynthetic organisms. The two proteins are functionally equivalent, but strongly differ in their global electrostatic charge. In fact, they are acidic in eukaryotes, but either neutral or basic in cyanobacteria. The difference in their electrostatic features is a critical factor in designing the purification procedure, which must be modified and adapted accordingly. This chapter reports the methods for producing (including cell cultures), isolating and purifying plastocyanin and cytochrome c6-which greatly differ in their isoelectric point-from a number of eukaryotic and prokaryotic organisms.
Navarro
José A
JA
Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla y Consejo Superior de Investigaciones Científicas, Américo Vespucio, Spain.
Hervás
Manuel
M
De la Rosa
Miguel A
MA
D020418:Algal Proteins / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000302:isolation & purification*; D000460:Chlorophyta / Q000737:chemistry; D002845:Chromatography / Q000379:methods; D000458:Cyanobacteria / Q000737:chemistry; D045365:Cytochromes c6 / Q000302:isolation & purification*; D010940:Plant Proteins / Q000302:isolation & purification*; D010970:Plastocyanin / Q000302:isolation & purification*; D018724:Spinacia oleracea / Q000737:chemistry
D020418:Algal Proteins; D001426:Bacterial Proteins; D045365:Cytochromes c6; D010940:Plant Proteins; D010970:Plastocyanin