Selective depletion of the Type I, Type II, and Type III isozymes of hexokinase in mammalian cells using small interfering RNAs.
Constructs based on the pSUPER vector [Science 296 (2002) 550] and encoding small interfering RNAs specific for the Type I, Type II, or Type III isozymes of mammalian (rat) hexokinase were prepared. Transfection of Chinese hamster ovary and HeLa cells with these vectors resulted in selective depletion of the respective isozymes. A Zeocin marker was incorporated into the modified pSUPER vector, permitting isolation of stably transfected cell lines selectively depleted of the respective isozyme.
Sui
Dexin
D
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA.
Wilson
John E
JE
D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006593:Hexokinase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D014162:Transfection
D007527:Isoenzymes; D034741:RNA, Small Interfering; D006593:Hexokinase