title: [Deuterium oxide as a stress factor to the methylotrophic bacterium Methylophilus sp].
abstract: The adaptation of the methylotrophic bacterium Methylophilus sp. B-7741 to growth in highly deuterated media was studied. For the first time, we showed the cross adaptation of bacterial cells to deuterated media and oxidative and osmotic stresses. The activity at catalase in deuterated cells was higher than in the control cells. Deuterated cell-free culture liquids showed protective effects on the growth of Methylophilus sp. B-7741 in deuterated media, which was manifested as an increase in the deuterated biomass yield. These data and the data available in the literature suggest that the mechanisms of bacterial cell adaptation to heavy water and to oxidative and osmotic stresses are similar.
authors.affiliation: Department of Biotechnology, Lomonosov State Academy of Fine Chemical Technology, pr. Vernadskogo 86, Moscow, 117571 Russia.
authors.forename: A N
authors.initials: AN
authors.lastname: Nevo
authors.forename: A B
authors.initials: AB
authors.lastname: Pshenichnikova
authors.forename: D A
authors.initials: DA
authors.lastname: Skladnev
authors.forename: V I
authors.initials: VI
authors.lastname: Shvets
mesh_terms: D000222:Adaptation, Physiological; D002374:Catalase / Q000378:metabolism; D017077:Culture Media, Conditioned; D017666:Deuterium Oxide; D020629:Methylophilus / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D009995:Osmosis; D018384:Oxidative Stress
chemical_list: D017077:Culture Media, Conditioned; D002374:Catalase; D017666:Deuterium Oxide