title: Second-harmonic optical coherence tomography.
abstract: Second-harmonic optical coherence tomography, which uses coherence gating of second-order nonlinear optical responses of biological tissues for imaging, is described and demonstrated. Femtosecond laser pulses were used to excite second-harmonic waves from collagen harvested from rat tail tendon and a reference non-linear crystal. Second-harmonic interference fringe signals were detected and used for image construction. Because of the strong dependence of second-harmonic generation on molecular and tissue structures, this technique imparts contrast and resolution enhancement to conventional optical coherence tomography.
authors.affiliation: Beckman Laser Institute, Department of Biomedical Engineering, University of California, Irvine, Irvine, California 92612, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.forename: Ivan
authors.initials: I
authors.lastname: Tomov
authors.forename: Yimin
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D001718:Birefringence; D003094:Collagen / Q000648:ultrastructure*; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D051381:Rats; D013623:Tail / Q000166:cytology*; D041623:Tomography, Optical Coherence / Q000379:methods*
chemical_list: D003094:Collagen