title: The CGG repeat and the FMR1 gene.
abstract: This review intends to provide the different DNA methods for diagnosis of the repeat in the FMR1 gene. The two DNA methods to determine the CGG repeat size are Southern blot hybridization and the polymerase chain reaction (PCR), including bisulfite treatment.
authors.affiliation: Department of Clinical Genetics, Erasmus MC, Rotterdam, The Netherlands.
authors.forename: Violeta
authors.initials: V
authors.lastname: Stoyanova
authors.forename: Ben A
authors.initials: BA
authors.lastname: Oostra
mesh_terms: D015139:Blotting, Southern; D017931:DNA Primers; D005260:Female; D051860:Fragile X Messenger Ribonucleoprotein 1; D006801:Humans; D008297:Male; D009419:Nerve Tissue Proteins / Q000235:genetics*; D010375:Pedigree; D016133:Polymerase Chain Reaction; D016601:RNA-Binding Proteins; D018911:Trinucleotide Repeats
chemical_list: D017931:DNA Primers; D051860:Fragile X Messenger Ribonucleoprotein 1; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins; C497683:FMR1 protein, human