[Study on the immunogenicity of HIV-1 gag vaccine].
OBJECTIVE
To detect the immunogenicity of HIV-1 gag DNA vaccine.


METHODS
The serum antibody titers of the immunized mice were detected by ELISA. The numbers of spleen's CD4(+) and CD8(+) T cells were analyzed by the fluorescent antibody staining and the cytotoxic activity was determined by lactate dehydrogenase (LDH) release assay.


RESULTS
The titers of the serum antibodies and the numbers and cytotoxic activity of spleen's T lymphocyte subsets had significant differences (P<0.05 and P<0.01, respectively) between pVAXGAG and pVAX1 vaccinated groups.


CONCLUSIONS
HIV-1 DNA vaccine plasmid pVAXGAG could elicit both the specific humoral and cellular immunities in BALB/c mice.
Jiang
Wen-Zheng
WZ
Key Laboratory of Genetic Engineering, Quartermaster University of PLA, Changchun 130062, China. wzjiang@hotmail.com
Jin
Ning-Yi
NY
Li
Zi-Jian
ZJ
Zhang
Li-Shu
LS
D016915:AIDS Vaccines / Q000276:immunology*; D000818:Animals; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005260:Female; D015683:Gene Products, gag / Q000276:immunology*; D015483:HIV Antibodies / Q000032:analysis; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D018655:Lymphocyte Count; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D019444:Vaccines, DNA / Q000276:immunology*
D016915:AIDS Vaccines; D015683:Gene Products, gag; D015483:HIV Antibodies; D019444:Vaccines, DNA