title: Removal of AMPA receptors (AMPARs) from synapses is preceded by transient endocytosis of extrasynaptic AMPARs.
abstract: AMPA receptors (AMPARs) are dynamically regulated at synapses, but the time course and location of their exocytosis and endocytosis are not known. Therefore, we have used ecliptic pHluorin-tagged glutamate receptor 2 to visualize changes in AMPAR surface expression in real time. We show that synaptic and extrasynaptic AMPARs respond very differently to NMDA receptor activation; there is a rapid internalization of extrasynaptic AMPARs that precedes the delayed removal of synaptic AMPARs.
authors.affiliation: Medical Research Council, Centre for Synaptic Plasticity, Department of Anatomy, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom.
authors.forename: Michael C
authors.initials: MC
authors.lastname: Ashby
authors.forename: Sarah A
authors.initials: SA
authors.lastname: De La Rue
authors.forename: G Scott
authors.initials: GS
authors.lastname: Ralph
authors.forename: James
authors.initials: J
authors.lastname: Uney
authors.forename: Graham L
authors.initials: GL
authors.lastname: Collingridge
authors.forename: Jeremy M
authors.initials: JM
authors.lastname: Henley
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D004705:Endocytosis / Q000502:physiology*; D049452:Green Fluorescent Proteins; D006624:Hippocampus / Q000166:cytology; D006863:Hydrogen-Ion Concentration; D008164:Luminescent Proteins / Q000235:genetics; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology; D051381:Rats; D018091:Receptors, AMPA / Q000235:genetics / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013569:Synapses / Q000378:metabolism*
chemical_list: D008164:Luminescent Proteins; D018091:Receptors, AMPA; D016194:Receptors, N-Methyl-D-Aspartate; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; C104722:glutamate receptor ionotropic, AMPA 2