title: Development of donor-specific microchimerism in liver transplant recipient with HLA-DRB1 and -DQB1 mismatch related to rejection episodes.
abstract: Migration of donor-derived cells to recipient tissues after liver transplantation has been suggested as a mechanism to induce and maintain allograft tolerance, although important issues remain including acute rejection posttransplantation mortality, and complications related to immunosuppressive therapy. We therefore examined the relation of rejection to chimerism based upon recipient and donor mismatch of HLA-DRB1 and -DQB1 alleles. Laboratory analysis of peripheral blood was performed before and 10 days to 16 months after liver transplantation in 32 recipients, using ganglion or spleen cell samples of respective donors. DNA was extracted for HLA-DRB1 and DQB1 allele typing using polymerase chain reactions with sequence-specific primers (PCR-SSP). Microchimerism was analyzed through nested PCR. Our results confirmed that patients with one or two mismatched HLA-DRB1 and-DQB1 alleles showed microchimerism and no rejection (P <.05). Microchimerism was present in 71.88% of the patients, and a significant association of rejection P <.05 was found when microchimerism was correlated to graft rejection. These results suggest that the presence of microchimerism may be associated with acceptance, tolerance and survival of the allograft.
authors.affiliation: Immunogenetics Transplant Laboratory, Clinical Pathology Department, School of Medical Sciences, Campinas, SP, Brazil.
authors.forename: M B
authors.initials: MB
authors.lastname: Araújo
authors.forename: L S
authors.initials: LS
authors.lastname: Leonardi
authors.forename: I F S F
authors.initials: IF
authors.lastname: Boin
authors.forename: M I
authors.initials: MI
authors.lastname: Leonardi
authors.forename: L A
authors.initials: LA
authors.lastname: Magna
authors.forename: E A
authors.initials: EA
authors.lastname: Donadi
authors.forename: M H S
authors.initials: MH
authors.lastname: Kraemer
mesh_terms: D006084:Graft Rejection / Q000453:epidemiology / Q000276:immunology*; D006683:HLA-DQ Antigens / Q000235:genetics / Q000276:immunology*; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics / Q000276:immunology*; D059811:HLA-DRB1 Chains; D006650:Histocompatibility Testing; D006801:Humans; D016031:Liver Transplantation / Q000276:immunology*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D014019:Tissue Donors; D018183:Transplantation Chimera
chemical_list: D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains