[The mechanism of KAI1 gene in inhibition of metastasis of primary pancreatic cancer].
OBJECTIVE
To study the effect on invasive and motor ability of MiaPaCa II pancreatic cancer cell transfected by recombinant plasmid pCMV-KAI1, alteration of KAI1 in the transfecting cancer cells, and the metastatic mechanism in the malignancy.


METHODS
MiaPaCa II pancreatic cancer cell was transfected with pCMV-KAI1 by liposome, the invasive and gelatinase ability of two pancreatic cancer cell lines were analyzed with transwell culture cell and polyacrylamided gel electrophoresis (PAGE) before and after KAI1 cDNA transfection.


RESULTS
After KAI1 cDNA transfected into MiaPaCa II, the migrative ability of passing through the membrane filter decreased evidently (P < 0.05). The width of negative staining band narrowed, the intensity level of tape weakened through PAGE. These results showed that reduced the ability of degrading basement membrane and extra-cell base and metastasis were influenced after MiaPaCa II pancreatic cancer cells were transfected.


CONCLUSIONS
The suppressed invasion and motor function of pancreatic cancer cells seems to influence their metastatic ability and thereby entrances the malignant potential of pancreatic cancer.
Guo
Xiao-zhong
XZ
Department of Gastroenterology, General Hospital of Shenyang Military Region, Shenyang 110016, China. guoxz@pub.sy.ln.cn
Xu
Jian-hua
JH
Liu
Min-pei
MP
Ren
Li-nan
LN
Wang
Di
D
Li
Hong-yu
HY
Wu
Chun-yan
CY
D015703:Antigens, CD / Q000235:genetics*; D045744:Cell Line, Tumor; D018093:Gelatinases / Q000378:metabolism; D006801:Humans; D051861:Kangai-1 Protein; D008562:Membrane Glycoproteins / Q000235:genetics*; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D010190:Pancreatic Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D010957:Plasmids / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D014162:Transfection
D015703:Antigens, CD; C497688:CD82 protein, human; D051861:Kangai-1 Protein; D008562:Membrane Glycoproteins; D011518:Proto-Oncogene Proteins; D018093:Gelatinases