title: Morphologic, immunohistochemical, and molecular characterization of hepatosplenic T-cell lymphoma in a dog.
abstract: A 13-year-old neutered male Jack Russell Terrier (Parson Russell Terrier) was presented to the Texas Veterinary Medical Center with a history of lethargy, depression, vomiting, and fever. The dog had mildly regenerative anemia, severe thrombocytopenia and low antithrombin activity. Marked splenomegaly was found on physical examination and imaging studies, and malignant round cell neoplasia and marked extramedullary hematopoiesis were diagnosed on aspirates of the spleen. The dog underwent exploratory laporatomy and splenectomy. Because of a rapid decline in clinical condition postsurgery, the dog was euthanized. Splenic and hepatic biopsies were submitted for histopathologic evaluation. A neoplastic population of round cells was found throughout the splenic parenchyma and within hepatic sinusoids. The neoplastic cells stained strongly positive for CD3 (T-cell marker) and were negative for CD79a (B-cell marker) and lysozyme (histiocytic marker). A diagnosis of T-cell lymphoma was confirmed by assessment of T-cell clonality using canine-specific polymerase chain reaction-based techniques. Although expression of the gammadelta T-cell receptor was not evaluated, this case shares many similarities with a rare syndrome in humans known as hepatosplenic gammadelta T-cell lymphoma.
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX., USA. ecienava@cvm.tamu.edu
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Cienava
authors.forename: Kirstin F
authors.initials: KF
authors.lastname: Barnhart
authors.forename: Raquel
authors.initials: R
authors.lastname: Brown
authors.forename: Joanne
authors.initials: J
authors.lastname: Mansell
authors.forename: Robert
authors.initials: R
authors.lastname: Dunstan
authors.forename: Kelly
authors.initials: K
authors.lastname: Credille
mesh_terms: D000818:Animals; D003937:Diagnosis, Differential; D004283:Dog Diseases / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D004285:Dogs; D017809:Fatal Outcome; D015329:Gene Rearrangement, T-Lymphocyte / Q000235:genetics; D007150:Immunohistochemistry / Q000662:veterinary; D008113:Liver Neoplasms / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D016399:Lymphoma, T-Cell / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics; D013156:Splenectomy / Q000662:veterinary; D013160:Splenic Neoplasms / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D013163:Splenomegaly / Q000209:etiology / Q000473:pathology / Q000601:surgery / Q000662:veterinary
chemical_list: D016692:Receptors, Antigen, T-Cell, gamma-delta