title: Hyphae formation of Candida albicans is regulated by polyamines.
abstract: Candida albicans generally grows in hyphae form in RPMI1640 medium. However, addition of 1,4-diamino-2-butanone (DAB), a competitive inhibitor of ornithine decarboxylase, decreased the amount of polyamines in C. albicans, and induced the proliferation of the C. albicans yeast form. The expression of CYR1 mRNA was significantly inhibited by the addition of DAB compared with that of the control. The amount of intracellular cAMP was also decreased by the addition of DAB. A specific adenylate cyclase inhibitor, cis-N-[2-phenylcyclopentyl]-azacyclotridec-1-en-2-amine (MDL-12,330A) promoted the growth of the yeast form. These results indicated that polyamines exist upstream of the adenylate cyclase-cAMP signal pathway and regulate the transformation of C. albicans.
authors.affiliation: Department of Microbiology, Tohoku Pharmaceutical University, Sendai, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Ueno
authors.forename: Makoto
authors.initials: M
authors.lastname: Fukumatsu
authors.forename: Ayako
authors.initials: A
authors.lastname: Ogasawara
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Watanabe
authors.forename: Takeshi
authors.initials: T
authors.lastname: Mikami
authors.forename: Tatsuzi
authors.initials: T
authors.lastname: Matsumoto
mesh_terms: D002176:Candida albicans / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D025301:Hyphae / Q000187:drug effects / Q000378:metabolism*; D011073:Polyamines / Q000378:metabolism* / Q000494:pharmacology; D011700:Putrescine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D011073:Polyamines; C014458:1,4-diaminobutanone; D011700:Putrescine