title: [Accelerated hydrophobic chromatography of plant extracts: a promising tool for study of biologically active polyphenols].
abstract: A simple and efficient technique for fast isolation and purification of a total polyphenol fraction from dry herbal plant extract ("Phytoprost" preparation) has been developed. The method is based on sequential cascade of 1.0-5.0 kDa ultrafiltration and the following subsequent column chromatography of resultant oligo/polymeric compounds on Phenyl-Sepharose 4B-CL. This novel procedure allows to obtain a highly purified (not more than 0.5-0.6% of aromatic and heterocyclic impurities) fraction of plant polyphenols in amounts required for pharmacological/biochemical screening (0.85 +/- 0.06 A280/g dry extract) within 8-9 hours.
authors.affiliation: I.M. Sechenov Moscow Medical Academy, Moscow, Russia 119881, Moscow, M. Trubetskaya, 8. markaria@rol.ru
authors.forename: A A
authors.initials: AA
authors.lastname: Markarian
authors.forename: T D
authors.initials: TD
authors.lastname: Dargaeva
authors.forename: R N
authors.initials: RN
authors.lastname: Aliautdin
authors.forename: V L
authors.initials: VL
authors.lastname: Burkov
mesh_terms: D002850:Chromatography, Gel / Q000379:methods; D005419:Flavonoids / Q000032:analysis*; D057927:Hydrophobic and Hydrophilic Interactions; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D059808:Polyphenols; D014462:Ultrafiltration
chemical_list: D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols