[Effect of titanium particles loading on the viability of mesenchymal stem cells after osteoblastic induction].
OBJECTIVE
To assess the effect of titanium particles on the proliferation of rMSCs after osteoblastic induction.


METHODS
MTT-assay and Confocal Laser Scanning Microscopy are utilized to detect rMSCs' proliferation rate and morphologic variety.


RESULTS
It was found that Ti particles loading suppressed the proliferation of osteogenesis behavior of rMSCs, but the down-regulation effect varied in different circle diameter, different concentration and different loading duration of Ti particles. Especially, among three different particles, the submicron Ti particles (0.9 micron) had the most suppressive effect on rMSCs. Under the same diameter condition, the inhibitory effect induced by Ti particles loading showed a manner dependent on the particles concentration and exposure duration. The reductive response from 1 g/L Ti was the earliest and most intense one to come forth. And the lower the concentration was in use, the weaker would be the reductive response. Furthermore, with the elongation of exposure to Ti particles, proliferation was steadily decreasing and its lowest level was at 32 h of exposure.


CONCLUSIONS
These findings demonstrated that Ti particles loading could suppress the viability of rMSCs' osteoblastic differentiation in a manner dependent on the circle diameter, particles concentration, and treatment time.
Cao
Hui
H
Institute of Biomedical Engineering, West China School of Preclinical and Foreasic Medicine, Sichuan University, Chengdu 610041, China.
Chen
Huai-qing
HQ
Wu
Jiang
J
Li
Liang
L
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003864:Depression, Chemical; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D018613:Microscopy, Confocal; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis / Q000187:drug effects; D010316:Particle Size; D011475:Prosthesis Failure; D051381:Rats; D014025:Titanium / Q000633:toxicity*
D014025:Titanium