[Analysis of methylation status of the promoter of mdr1 gene in K562 and K562/DNR cells].
OBJECTIVE
To analyze the methylation patterns of mdr1 gene promotor in both K562 cell and K562/DNR cells and the relationship between promotor methylation and P-gp expression.


METHODS
mdr1 gene expression was analyzed by flow cytometry and RT-PCR, methylation status of the promotor of mdr1 gene by bisulfite-sequencing (including two GC-box).


RESULTS
mdr1 gene was found methylated at GC-box and not expressed in K562 cells, but unmethylated and expressed respectively in K562/DNR cells.


CONCLUSIONS
The methylation patterns of mdr1 gene promotor in K562/DNR and K562 cells were different. mdr1 gene silencing was associated with the promotor methylation.
Gao
Feng
F
Department of Medical Oncology, The First Affiliated Hospital, China Medical University, Shenyang 110001, China.
Zhang
Jing-dong
JD
Liu
Yun-peng
YP
Lu
Xiang-lan
XL
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D019175:DNA Methylation; D005434:Flow Cytometry; D020868:Gene Silencing; D006801:Humans; D020014:K562 Cells; D011401:Promoter Regions, Genetic / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1