[Reversing effect of histamine on neuron death induced by N-methyl-D-aspartate].
To determine the effect of histamine on N-methyl-D-aspartate (NMDA) induced neuron death and to elucidate its mechanism.



The primary cortical cell culture was adopted. Neuron morphology and MTT assay were used to evaluate the drugs effects.



Histamine at doses of 10(-4) 10(-6) 10(-7) 10(-8) mol/L reversed the neuron death induced by NMDA (50 micromol/L) for 3 h. The protection of histamine peaked at doses of 10(-4) mol/L and 10(-7)mol/L. The effect of histamine of 10(-7) mol/L was reversed only by cimetidine an H(2)receptor antagonist. However, the effect of histamine of 10(-4) mol/L was reversed only by pyrilamine but not cimetidine.



Histamine could reduce neuron death induced by NMDA; its protection at a low dose might be mediated by H(2)receptor, and at a high dose by H(1)receptor.
Dai
Hai-bin
HB
Department of Pharmacology, College of Medicine, Zhejiang University, Hangzhou 310031, China.
Chen
Zhong
Z
Huang
Yu-wen
YW
Ge
Qiu-fu
QF
Zhang
Zhong-miao
ZM
Wei
Er-qing
EQ
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006632:Histamine / Q000494:pharmacology*; D016202:N-Methylaspartate / Q000633:toxicity*; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011969:Receptors, Histamine H1 / Q000502:physiology; D011970:Receptors, Histamine H2 / Q000502:physiology
D018696:Neuroprotective Agents; D011969:Receptors, Histamine H1; D011970:Receptors, Histamine H2; D016202:N-Methylaspartate; D006632:Histamine