beta-hydroxybutyrate induces acute pulmonary endothelial dysfunction in rabbits.
The authors examined the effects of high ketone body and glucose concentrations on endothelial cell (EC) function in perfused rabbit lungs. beta-Hydroxybutyrate (beta OHB), at 5 mM, decreased endothelial angiotensin-converting enzyme (eACE) activity, whereas 25 mM glucose (HG), 1 mM beta OHB, or 10 mM acetoacetate (AcAc) did not. Dry to wet weight ratios were also reduced in lungs perfused with 5 mM beta OHB, but not with AcAc. beta OHB, at 5 mM, caused massive hemorrhage and interstitial and alveolar neutrophil infiltration; AcAc only produced engorgement of septal capillaries. Thus, pulmonary EC dysfunction occurs in rabbit lungs acutely perfused with beta OHB, but not with AcAc or glucose.
McCloud
Laryssa L
LL
Vascular Biology Center, Medical College of Georgia, Augusta, GA 30912-2500, USA. lmccloud@mail.mcg.edu
Parkerson
James B
JB
Freant
Lawrence
L
Hoffman
William H
WH
Catravas
John D
JD
D020155:3-Hydroxybutyric Acid / Q000494:pharmacology*; D000208:Acute Disease; D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D016883:Diabetic Ketoacidosis / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D007657:Ketone Bodies / Q000378:metabolism; D008168:Lung / Q000098:blood supply / Q000187:drug effects* / Q000473:pathology; D008171:Lung Diseases / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D011652:Pulmonary Circulation / Q000187:drug effects; D011817:Rabbits
D007657:Ketone Bodies; D007703:Peptidyl-Dipeptidase A; D020155:3-Hydroxybutyric Acid