Identification of hibernating myocardium by acoustic microscopy.
Hibernating myocardium is viable myocardium that recovers after revascularization. The observation of loss of contractile proteins (myofibrils) and accumulation of glycogen in hibernating cardiomyocytes provide the basis for diagnosing hibernating myocardium. In this pilot study, acoustic microscopy was used to identify the cellular structure of normal vs. hibernating myocardium. Sections cut at 5-microm of archival paraffin blocks on glass slides were used for this study. Acoustic microscopy of normal cardiomyocytes showed intracellular linear echoes suggestive of myofibrils, and cardiomyocytes of hibernating myocardium revealed absence of myofibrils and dense intracellular echoes that corresponded to glycogen accumulation on optical microscopy. This modality of visualization allows a definitive diagnosis of hibernating myocardium.
Ng
Daniel Wing Chong
DW
Division of Cardiology, LAC+USC Medical Center, University of Southern California School of Medicine, Los Angeles, CA, USA.
Sathish
Shamachary
S
Khan
Aameera
A
Chandrasoma
Parakrama
P
Wijns
William
W
Chandraratna
P A N
PA
D006003:Glycogen / Q000032:analysis; D006801:Humans; D033401:Microscopy, Acoustic / Q000379:methods*; D017682:Myocardial Stunning / Q000000981:diagnostic imaging*; D032383:Myocytes, Cardiac / Q000000981:diagnostic imaging / Q000502:physiology; D009210:Myofibrils / Q000000981:diagnostic imaging; D010865:Pilot Projects; D014617:Vacuoles / Q000000981:diagnostic imaging
D006003:Glycogen