title: Bovine glue (BioGlue) is catabolized by enzymatic reaction in the vascular dog model.
abstract: OBJECTIVE
  The aim of the study is to explore the feasibility, patency, and histologic changes of a sutureless vascular anastomotic technique using biological glue as sole fixation method.
  
  
  METHODS
  Eight mongrel dogs (+/-15 kg) underwent direct reanastomosis of their transsected iliac arteries. Both ends were placed on a 5-mm balloon and the anastomosis was secured with biological glue (BioGlue, Cryolife, Kennesaw, GA). No intravascular suture material was used. All survivors were angiographically controlled for patency after 6 weeks and 3 months. Then the animals were euthanized and tissues were obtained for histologic and pathologic examination by light and electron microscopy.
  
  
  RESULTS
  All procedures were successful except for 1 animal that died of uncontrollable bleeding at the anastomotic site. All first-time angiographically controlled grafts except three were patent. One animal showed manifest signs of fungal infection. Histology detected early granulocyte infiltration with an important enzymatic reaction adjacent to the surface of glue. Later on, the glue gradually regressed to disappear completely. Fibroblastic neointimal lining was noticed in most of the anastomoses, with some marked differences in the endothelium compared with normal.
  
  
  CONCLUSIONS
  Good permeability (57%) was observed in this new sutureless anastomotic technique in the canine model. In contrast to previous reported studies we noticed a clear enzymatic breakdown of the glue before total disappearance. It is not yet known to what extend use of the bovine glue was responsible for this phenomenon.
authors.affiliation: Heart Centre University Hospital Ghent, Ghent, Belgium.
authors.forename: Yves
authors.initials: Y
authors.lastname: Van Belleghem
authors.forename: Ramses G
authors.initials: RG
authors.lastname: Forsyth
authors.forename: Kishan
authors.initials: K
authors.lastname: Narine
authors.forename: Annelies
authors.initials: A
authors.lastname: Moerman
authors.forename: Yves
authors.initials: Y
authors.lastname: Taeymans
authors.forename: Guido J
authors.initials: GJ
authors.lastname: Van Nooten
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D004285:Dogs; D005260:Female; D005549:Foreign-Body Reaction / Q000209:etiology / Q000473:pathology; D006098:Granulocytes / Q000201:enzymology* / Q000473:pathology; D007083:Iliac Artery / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery*; D011506:Proteins / Q000737:chemistry; D011859:Radiography; D014014:Tissue Adhesives / Q000737:chemistry; D014654:Vascular Patency; D014945:Wound Healing
chemical_list: C472676:Bio-glue; D011506:Proteins; D014014:Tissue Adhesives