title: PKA-phosphorylation of PDE4D3 facilitates recruitment of the mAKAP signalling complex.
abstract: mAKAP (muscle-selective A-kinase-anchoring protein) co-ordinates a cAMP-sensitive negative-feedback loop comprising PKA (cAMP-dependent protein kinase) and the cAMP-selective PDE4D3 (phosphodiesterase 4D3). In vitro and cellular experiments demonstrate that PKA-phosphorylation of PDE4D3 on Ser-13 increases the affinity of PDE4D3 for mAKAP. Our data suggest that activation of mAKAP-anchored PKA enhances the recruitment of PDE4D3, allowing for quicker signal termination.
authors.affiliation: Howard Hughes Medical Institute and Vollum Institute, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239-3098, USA.
authors.forename: Jennifer J
authors.initials: JJ
authors.lastname: Carlisle Michel
authors.forename: Kimberly L
authors.initials: KL
authors.lastname: Dodge
authors.forename: Wei
authors.initials: W
authors.lastname: Wong
authors.forename: Nicole C
authors.initials: NC
authors.lastname: Mayer
authors.forename: Lorene K
authors.initials: LK
authors.lastname: Langeberg
authors.forename: John D
authors.initials: JD
authors.lastname: Scott
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D048868:Adaptor Proteins, Signal Transducing / Q000502:physiology*; D001665:Binding Sites; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4; D004789:Enzyme Activation / Q000502:physiology; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000196:embryology / Q000201:enzymology; D018716:Molecular Mimicry / Q000502:physiology; D010446:Peptide Fragments / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D010766:Phosphorylation; D011485:Protein Binding / Q000502:physiology; D025941:Protein Interaction Mapping; D012694:Serine / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D010446:Peptide Fragments; D010455:Peptides; D012694:Serine; D017868:Cyclic AMP-Dependent Protein Kinases; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4