Preparation of native and recombinant light-harvesting chlorophyll-a/b complex.
Procedures to isolate native light-harvesting chlorophyll-a/b complex (LHCIIb) and to reconstitute recombinant LHCIIb are described. Separation of trimeric from monomeric forms and free pigment by sucrose density-gradient ultracentrifugation can be applied to both native and reconstituted complexes. The preparations are characterized by their pigment composition, protein pattern and spectral properties.
Rühle
Wolfgang
W
Institut f. Allgemeine Botanik der Johannes-Gutenberg-Universität, Mainz, Germany.
Paulsen
Harald
H
D019281:Dimerization; D045342:Light-Harvesting Protein Complexes / Q000235:genetics / Q000302:isolation & purification*; D018532:Pisum sativum / Q000737:chemistry / Q000166:cytology; D045332:Photosystem II Protein Complex / Q000235:genetics / Q000302:isolation & purification*; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification*; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D020524:Thylakoids / Q000737:chemistry
D045342:Light-Harvesting Protein Complexes; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D011994:Recombinant Proteins