title: Excitation-emission fluorimeter based on linear interference filters.
abstract: We describe the design, properties, and performance of an excitation-emission (EE) fluorimeter that enables spectral characterization of an object simultaneously with respect to both its excitation and its emission properties. Such devices require two wavelength-selecting elements, one in the optical path of the excitation broadband light to obtain tunable excitation and the other to analyze the resulting fluorescence. Existing EE instruments are usually implemented with two monochromators. The key feature of our EE fluorimeter is that it employs lightweight and compact linear interference filters (LIFs) as the wavelength-selection elements. The spectral tuning of both the excitation and the detection LIFs is achieved by their mechanical shift relative to each other by use of two computer-controlled linear step motors. The performance of the LIF-based EE fluorimeter is demonstrated with the fluorescent spectra of various dyes and their mixtures.
authors.affiliation: Department of Electrical and Computer Engineering, State University of New York at Stony Brook, Stony Brook, New York 11794-2350, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Gouzman
authors.forename: Nadia
authors.initials: N
authors.lastname: Lifshitz
authors.forename: Serge
authors.initials: S
authors.lastname: Luryi
authors.forename: Oleg
authors.initials: O
authors.lastname: Semyonov
authors.forename: Dmitry
authors.initials: D
authors.lastname: Gavrilov
authors.forename: Vyacheslav
authors.initials: V
authors.lastname: Kuzminskiy