title: Genomic cloning and expression of vitellogenin gene from the self-fertilizing fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae).
abstract: We cloned the vitellogenin gene from the self-fertilizing fish Rivulus marmoratus, and sequenced 12,326 bp. The number of exons of R. marmoratus and rainbow trout vitellogenin genes were different, and also the splicing junctions are different throughout most of the exons and introns but the amino acid similarity of R. marmoratus vitellogenin gene to other species was rather high. In promoter region of R. marmoratus vitellogenin gene, there were several E2 binding sites and the estrogen response element (ERE). We discuss here the gene structure and expression of R. marmoratus vitellogenin gene.
authors.affiliation: Department of Biochemistry, College of Medicine, Hanyang University, Seoul 133-791, Republic of Korea.
authors.forename: Il-Chan
authors.initials: IC
authors.lastname: Kim
authors.forename: Sung Yeoul
authors.initials: SY
authors.lastname: Chang
authors.forename: Tim D
authors.initials: TD
authors.lastname: Williams
authors.forename: Young
authors.initials: Y
authors.lastname: Ja Kim
authors.forename: Yong-Dal
authors.initials: YD
authors.lastname: Yoon
authors.forename: Yong-Sung
authors.initials: YS
authors.lastname: Lee
authors.forename: Eun-Ho
authors.initials: EH
authors.lastname: Park
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003532:Cyprinodontiformes / Q000235:genetics*; D017931:DNA Primers; D040461:Gene Components; D015698:Genomic Library; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D020218:Response Elements / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014819:Vitellogenins / Q000235:genetics*
chemical_list: D017931:DNA Primers; D014819:Vitellogenins