Use of herpesvirus papio 2 as an alternative antigen in immunoblotting assay for B virus diagnosis.
Herpesvirus papio 2 (HVP2), which infects baboons, is much more closely related genetically and antigenically to monkey B virus (BV) than to human herpes simplex virus 1(HSV1) and other related herpes viruses. The usefulness of HVP2 as an alternative test antigen in immunoblotting assays to detect BV-antibody in macaque monkey sera was assessed. Six HVP2 proteins reacted with BV-positive sera in immunoblotting. No specific bands could be detected with BV-negative sera. These results show the usefulness of HVP2 antigen as an alternative and safer antigen than authentic BV antigen in detecting BV antibody in immunoblotting.
Tanaka
Seiichi
S
Division of Laboratory Animal Sciences, Department of Life Science, Institute of Scientific Research, Oita University, Hasama-machi, Japan.
Mannen
Kazuaki
K
Sato
Hiroshi
H
D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification; D000956:Antigens, Viral / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D006568:Herpesvirus 1, Cercopithecine / Q000276:immunology / Q000302:isolation & purification*; D015151:Immunoblotting / Q000379:methods*; D008251:Macaca; D008992:Monkey Diseases / Q000175:diagnosis* / Q000821:virology*; D018139:Simplexvirus / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral