title: [In vitro and in vivo study on proliferation of adnoid cystic carcinoma cell lines after nm23-h1. Introduction].
abstract: OBJECTIVE
  To study the effect of nm23-h1 transfection on proliferation characteristics of adnoid carcinoma cell lines in vitro and in vivo.
  
  
  METHODS
  In vitro ACC-M cell lines were incubated after putative anti-metastatic gene nm23-h1 was introduced into the cells with the help of G418 selective incubation base. The ACC-M cells were transplanted into 10 BALB/C nude mices subcutaneously and non-transfected cell lines were taken as control. Immunohisto chemistry and Ki67 antibody were employed to study the proliferation character of cell crawling pieces and paraffin-bedded slice, meanwhile, the solid tumor of both groups were prepared for flow cytometry(FCM).
  
  
  RESULTS
  Transfected cells grew slower than non-transfected cells and this trend became more obvious as passages passed on. In vitro the expression of Ki67 of transfected cells was little stronger than non-transfected cells, while the expression of Ki67 in solid slices was almost negative in both groups. Transfected cells presented slower growth than non-transfected cells in the early stage (2 weeks) and 2 weeks later there was no obvious difference in size(P > 0.05). FCM value accorded well with the result.
  
  
  CONCLUSIONS
  Introduction of nm23-h1 into the ACC-M cell lines may have transient inhibitory effects on its proliferation.
authors.affiliation: Dept. of Otolaryngology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.forename: Yu-ming
authors.initials: YM
authors.lastname: Wen
authors.forename: Li-hong
authors.initials: LH
authors.lastname: Wang
authors.forename: Zhan
authors.initials: Z
authors.lastname: Yang
authors.forename: Rui-lin
authors.initials: RL
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis; D003528:Carcinoma, Adenoid Cystic / Q000235:genetics / Q000473:pathology*; D002455:Cell Division; D045744:Cell Line, Tumor; D005260:Female; D015316:Genetic Therapy; D019394:Ki-67 Antigen / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000235:genetics / Q000473:pathology*; D054778:NM23 Nucleoside Diphosphate Kinases; D009368:Neoplasm Transplantation; D009701:Nucleoside-Diphosphate Kinase; D011506:Proteins / Q000235:genetics*; D014162:Transfection
chemical_list: D000906:Antibodies; D019394:Ki-67 Antigen; D054778:NM23 Nucleoside Diphosphate Kinases; D011506:Proteins; C517939:Nme1 protein, mouse; D009701:Nucleoside-Diphosphate Kinase