Oral plasmid DNA delivery systems for genetic immunisation.
The use and optimisation of plasmid DNA delivery systems for the purposes of eliciting transgene specific immune responses to orally administered DNA encoded antigen represents a significant challenge. Here, we have outlined a multicomponent polymer modified liposomal delivery system that offers potential for oral administration of plasmid DNA. It is shown that the polymer/liposome formulated DNA is able to elicit markedly enhanced transgene specific cytokine production following in vitro restimulation of splenocytes with recombinant antigen. This is discussed with reference to recent publications and the potential of plasmid DNA delivery systems for the purposes of genetic immunisation, as reported in selected literature, is assessed.
Somavarapu
S
S
Centre for Drug Delivery Research, School of Pharmacy, University of London, UK.
Bramwell
V W
VW
Alpar
H O
HO
D000284:Administration, Oral; D000818:Animals; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000276:immunology; D007371:Interferon-gamma / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D019344:Lactic Acid / Q000737:chemistry; D008081:Liposomes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry; D019076:Transgenes; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology*
D006514:Hepatitis B Surface Antigens; D015850:Interleukin-6; D008081:Liposomes; D011108:Polymers; D019444:Vaccines, DNA; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D007371:Interferon-gamma; D011142:Polyvinyl Alcohol