Inhibition of virus production in JC virus-infected cells by postinfection RNA interference.
RNA interference has been applied for the prevention of virus infections in mammalian cells but has not succeeded in eliminating infections from already infected cells. We now show that the transfection of JC virus-infected SVG-A human glial cells with small interfering RNAs that target late viral proteins, including agnoprotein and VP1, results in a marked inhibition both of viral protein expression and of virus production. RNA interference directed against JC virus genes may thus provide a basis for the development of new strategies to control infections with this polyomavirus.
Orba
Yasuko
Y
Laboratory of Molecular and Cellular Pathology, Hokkaido University School of Medicine, Kita-ku, Sapporo 060-8638, Japan.
Sawa
Hirofumi
H
Iwata
Hiroshi
H
Tanaka
Shinya
S
Nagashima
Kazuo
K
D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D002461:Cell Line, Transformed; D015967:Gene Expression Regulation, Viral; D006801:Humans; D007577:JC Virus / Q000472:pathogenicity* / Q000502:physiology*; D009457:Neuroglia / Q000821:virology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D054334:Viral Regulatory and Accessory Proteins; D014779:Virus Replication; D000086082:Viroporin Proteins
D036022:Capsid Proteins; D034741:RNA, Small Interfering; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C062481:VP1 protein, polyomavirus; C042559:agnoprotein, polyomavirus; D000086082:Viroporin Proteins