Biosensor based on Langmuir-Blodgett films of poly(3-hexyl thiophene) for detection of galactose in human blood.
An amperometric biosensor was developed to estimate galactose in human blood serum. Monolayers of poly(3-hexyl thiophene) were placed on glass plates coated with indium tin oxide formed by dispensing a mixed solution of stearic acid in chloroform on to a water sub-phase. Galactose oxidase was mixed with poly(3-hexyl thiophene)/stearic acid in chloroform and dispensed on to the air-water interface of Langmuir-Blodgett trough. These monolayers were transferred on to glass plates which were used as working electrodes with platinum as a reference electrode. The amperometric galactose biosensor thus fabricated had a linear response from 0.05 to 0.5 g galactose l(-1) in blood serum. The normal level in blood is < 0.05 g galactose l(-1) in adults and 0-0.2 g galactose l(-1) in infants. In case of galactosemia, this increases to above 0.2 g galactose l(-1) in infants.
Sharma
Sandeep K
SK
Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India.
Singhal
Rahul
R
Malhotra
B D
BD
Sehgal
Neeta
N
Kumar
Ashok
A
D000328:Adult; D015374:Biosensing Techniques / Q000379:methods*; D020099:Coated Materials, Biocompatible; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000378:metabolism; D005690:Galactose / Q000097:blood*; D006801:Humans; D011108:Polymers / Q000737:chemistry*; D013876:Thiophenes / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D011108:Polymers; D013876:Thiophenes; C066730:polythiophene; D005690:Galactose