Expression of connexins in human preimplantation embryos in vitro.
Intercellular communication via gap junctions is required to coordinate developmental processes in the mammalian embryo. We have investigated if the connexin (Cx) isoforms known to form gap junctions in rodent preimplantation embryos are also expressed in human embryos, with the aim of identifying species differences in communication patterns in early development. Using a combination of polyA PCR and immunocytochemistry we have assessed the expression of Cx26, Cx31, Cx32, Cx40, Cx43 and Cx45 which are thought to be important in early rodent embryos. The results demonstrate that Cx31 and Cx43 are the main connexin isoforms expressed in human preimplantation embryos and that these isoforms are co-expressed in the blastocyst. Cx45 protein is expressed in the blastocyst but the protein may be translated from a generally low level of transcripts: which could only be detected in the PN to 4-cell embryos. Interestingly, Cx40, which is expressed by the extravillous trophoblast in the early human placenta, was not found to be expressed in the blastocyst trophectoderm from which this tissue develops. All of the connexin isoforms in human preimplantation embryos are also found in rodents pointing to a common regulation of these connexins in development of rodent and human early embryos and perhaps other species.
Bloor
Debra J
DJ
School of Biological Sciences, University of Manchester, Manchester, M13 9PT, UK. djbloor@btopenworld.com
Wilson
Yvonne
Y
Kibschull
Mark
M
Traub
Otto
O
Leese
Henry J
HJ
Winterhager
Elke
E
Kimber
Susan J
SJ
D001755:Blastocyst / Q000737:chemistry* / Q000378:metabolism*; D000072259:Connexin 26; D018031:Connexin 43 / Q000235:genetics / Q000276:immunology; D017630:Connexins / Q000235:genetics* / Q000276:immunology; D046508:Culture Techniques; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D011247:Pregnancy; D000097002:Gap Junction beta-1 Protein
D000072259:Connexin 26; D018031:Connexin 43; D017630:Connexins; D000097002:Gap Junction beta-1 Protein; C000607042:GJB2 protein, human; C082920:connexin 45; C068044:GJB3 protein, human