An improved method for analyzing coenzyme Q homologues and multiple detection of rare biological samples.
We have developed a simple method for the estimation of coenzyme Q homologues, neurotransmitters, metal ions, lipid peroxidation, gene expression, and DNA fragmentation simultaneously from genetically engineered mice brain regions and cultured neurons. The primary objective of this study was to improve conventional time-consuming, cumbersome, and less efficient procedures, and reduce the cost of conducting kinetic studies in rare biological samples. The improved method is novel, precise, efficient, accurate, sensitive, economical, versatile, and highly reproducible. The recovery and shelf life of coenzyme Q homologues was significantly increased and the chromatograms exhibited reduced background and retention times. It is envisaged that in addition to coenzyme Q homologues, the improved method could be utilized for the multiple analyses of DNA, RNA and proteins from clinically significant biopsy and autopsy samples.
Sharma
Sushil K
SK
Department of Pharmacology, Physiology, & Therapeutics, University of North Dakota School of Medicine & Health Sciences, 501 North Columbia Road, Grand Forks, ND 58203, USA. skumar@medicine.nodak.edu
Ebadi
Manuchair
M
D000818:Animals; D001923:Brain Chemistry / Q000235:genetics*; D002460:Cell Line; D002845:Chromatography / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D053938:DNA Fragmentation / Q000235:genetics; D005260:Female; D015870:Gene Expression / Q000235:genetics; D015227:Lipid Peroxidation / Q000235:genetics; D008297:Male; D008668:Metallothionein / Q000172:deficiency / Q000235:genetics; D008670:Metals / Q000032:analysis / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000235:genetics; D009448:Neurochemistry / Q000295:instrumentation / Q000379:methods*; D018377:Neurotransmitter Agents / Q000032:analysis / Q000378:metabolism; D009696:Nucleic Acids / Q000032:analysis* / Q000235:genetics; D010472:Perchlorates / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012985:Software Design; D051843:Synucleins; D013997:Time Factors; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000032:analysis* / Q000378:metabolism
D008670:Metals; D009419:Nerve Tissue Proteins; D018377:Neurotransmitter Agents; D009696:Nucleic Acids; D010472:Perchlorates; D051843:Synucleins; D014451:Ubiquinone; D008668:Metallothionein