Synthesis and immunogenicity of polysaccharide-protein conjugate composed of galactoglucoxylomannan of Cryptococcus laurentii.
Galactoglucoxylomannan (GalGXMan) antigen of Cryptococcus laurentii was conjugated to protein carrier by a simple one step reaction. The conjugate was immunogenic in rabbits and reinjection elicited booster response with significant increase of serum IgG (H+L) level. Induction of this Ig-isotype was confirmed in experiments with Protein A. Effectiveness of immune serum to inhibit the growth of C. laurentii was demonstrated. The results indicate that the prepared conjugate could be considered as effective immunogen with potential for incorporation in the vaccine.
Bystrický
Slavomír
S
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 842 38 Bratislava, Slovakia. chembyst@savba.sk
Paulovicová
Ema
E
Machová
Eva
E
D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood; D003454:Cryptococcus / Q000737:chemistry / Q000254:growth & development* / Q000276:immunology*; D005657:Fungal Vaccines / Q000008:administration & dosage / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008351:Mannans / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits; D012709:Serum Albumin / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D018074:Vaccines, Conjugate / Q000008:administration & dosage / Q000276:immunology*
D000908:Antibodies, Fungal; D005657:Fungal Vaccines; D007106:Immune Sera; D007074:Immunoglobulin G; D008351:Mannans; D012709:Serum Albumin; D018074:Vaccines, Conjugate; C432127:galactoglucoxylomannan