[Mast cells in cellular leiomyoma and endometrial stromal sarcoma of the uterus].
OBJECTIVE
To study the role of mast cells in the differential diagnosis of cellular leiomyoma and endometrial stromal sarcoma of uterus and its mechanism.


METHODS
Using SP immunohistochemical technique, the expression of proliferating cell nuclear antigen (PCNA) and mast cells in 25 cellular leiomyoma (CL) and 26 endometrial stromal sarcoma (ESS) of uterus were examined. The expression of estrogen receptor (ER) and CD44v3 in cellular leiomyoma was also studied.


RESULTS
The expression of PCNA was not significantly different from CL or ESS (P > 0.05), while mast cell count was statistically different between them (P < 0.01). Using a value of less than 7 mast cells per high power field was useful for the diagnosis of ESS, yielding 100% sensitivity and 92.0% specificity. There was a positive correlation between the mast cell count and CD44v3 in CL (r(s) = 0.589, P < 0.01), though no correlation was observed between mast cell count and PCNA or ER.


CONCLUSIONS
Number of mast cells is valuable for the discrimination of CL from ESS in the uterus. The mechanism and the role of higher quantity of mast cells in CL need further study.
Zhu
Xue-Qiong
XQ
Department of Gynecology, Women's Hospital, Medical College of Zhejiang University, Hangzhou 310006, China.
Shi
Yi-Fu
YF
Zhou
Cai-Yun
CY
D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis; D007889:Leiomyoma / Q000737:chemistry / Q000473:pathology*; D008407:Mast Cells / Q000473:pathology*; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011960:Receptors, Estrogen / Q000032:analysis; D018203:Sarcoma, Endometrial Stromal / Q000737:chemistry / Q000473:pathology*; D014594:Uterine Neoplasms / Q000737:chemistry / Q000473:pathology*
C121450:CD44V3,8-10; D018960:Hyaluronan Receptors; D018809:Proliferating Cell Nuclear Antigen; D011960:Receptors, Estrogen