Microbial degradation of quinoline: kinetics study with Burkholderia picekttii.
OBJECTIVE
To investigate the kinetics of quinoline biodegradation by Burkholderia pickttii, a gram negative rod-shaped aerobe, isolated in our laboratory.


METHODS
HPLC (Hewlett-Packard model 5050 with an UV detector) was used for the analysis of quinoline concentration. GC/MS method was used to identify the intermediate metabolites of quinoline degradation.


RESULTS
The biodegradation of quinoline was inhibited by quinoline at a high concentration, and the degradation process could be described by the Haldane model. The kinetic parameters based on Haldane substrate inhibition were evaluated. The values were vmax = 0.44 h(-1), K(S) = 166.7 mg/L, Ki = 650 mg/L, respectively. The quinoline concentration to avoid substrate inhibition was inferred theoretically and determined to be 329 mg/L.


CONCLUSIONS
The biodegradation of quinoline conforms to the Haldane inhibition model and the main intermediate metabolite of quinoline biodegradation is 2-hydroxy-quinoline.
Wang
Jian-Long
JL
Laboratory of Environmental Technology, INET, Tsinghua University, Beijing 100084, China. wangjl@tsinghua.edu.cn
Wu
Wei-Zhong
WZ
Zhao
Xuan
X
D001673:Biodegradation, Environmental; D018533:Biomass; D019117:Burkholderia / Q000254:growth & development* / Q000302:isolation & purification; D004785:Environmental Pollutants / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D007700:Kinetics; D011804:Quinolines / Q000032:analysis*; D012722:Sewage / Q000382:microbiology
D004785:Environmental Pollutants; D011804:Quinolines; D012722:Sewage; C037219:quinoline