title: Antifungal activity of Bacillus subtilis 355 against wood-surface contaminant fungi.
abstract: A strain of Bacillus subtilis was examined for antifungal activity against phytopathogenic and wood-surface contaminant fungi. The bacterium was grown in five culture media with different incubation times in order to study cell development, sporulation, and the production of metabolites with antifungal activity. The anti-sapstain and anti-mould activity of the bacterium grown in yeast extract glucose broth (YGB) medium in wood was also evaluated. In YGB, the bacterium inhibited the growth of several fungi and displayed a broader spectrum of activity than in the other media tested. A relationship between bacterial spore production and the formation of metabolites with antifungal activity was detected. YGB medium displayed effective control in wood block tests. YGB medium was extracted with solvents of increasing polarity and the dry residues were applied to silicagel plates, resolved with the appropriate solvent and sprayed with different solutions, detecting the presence, of amines, and higher alcohols. The bioautographic method revealed the presence of at least two active compounds against the blue-stain fungus Cladosporium cucumerinum.
authors.affiliation: Departamento de Tecnologia de Indústrias Químicas, Instituto Nacional de Engenharia e Tecnologia Industrial, Estrada ao Paço do Lumiar, 1699-038 Lisbon, Portugal. sonia.feio@ineti.pt
authors.forename: Sonia Savluchinske
authors.initials: SS
authors.lastname: Feio
authors.forename: Ana
authors.initials: A
authors.lastname: Barbosa
authors.forename: Manuela
authors.initials: M
authors.lastname: Cabrita
authors.forename: Lina
authors.initials: L
authors.lastname: Nunes
authors.forename: Alexandra
authors.initials: A
authors.lastname: Esteves
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Roseiro
authors.forename: Maria João Marcelo
authors.initials: MJ
authors.lastname: Curto
mesh_terms: D000898:Antibiosis; D000935:Antifungal Agents / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D001681:Biological Assay; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005658:Fungi / Q000187:drug effects; D014934:Wood
chemical_list: D000935:Antifungal Agents; D017077:Culture Media, Conditioned