Characterization of avipoxviruses from wild birds in Norway.
Avipoxviruses from different geographic regions of the world have been characterized to study their genetic and biological properties, but so far, no such work has been performed on Norwegian isolates. Lesions suggestive of avian pox, found on a Norwegian wild sparrow (Passer domesticus) and wood pigeon (Palumbus palumbus), were obtained in 1972 and 1996, respectively. Histologically, these lesions were demonstrated to be characteristic of poxvirus infections and the poxvirus was observed using an electron microscope. The resulting viruses were propagated in chicken embryo fibroblast cells. Restriction fragment length polymorphism of genomes from 2 Norwegian isolates and fowl pox vaccine strain, generated by BamHI, revealed a high degree of heterogeneity among the isolates. The profiles of avipoxviruses isolated from wild birds were clearly distinct from each other and also to the fowl poxvirus strain. Furthermore, chickens experimentally infected with pigeon poxvirus had higher antibody titers and extensive lesions compared to other isolates. This may suggest that pigeon poxvirus is more virulent than the other isolates.
Weli
Simon Chioma
SC
Department of Microbiology and Virology, University of Tromsø, Brevika, N-9037 Tromsø, Norway. simonw@fagmed.uit.no
Okeke
Malachy Ifeanyi
MI
Tryland
Morten
M
Nilssen
Oivind
O
Traavik
Terje
T
D000818:Animals; D000835:Animals, Wild / Q000821:virology; D018150:Avipoxvirus / Q000145:classification / Q000302:isolation & purification / Q000472:pathogenicity / Q000648:ultrastructure*; D001715:Bird Diseases / Q000473:pathology / Q000821:virology*; D001717:Birds; D002645:Chickens; D010856:Columbidae / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D008854:Microscopy, Electron / Q000662:veterinary; D009664:Norway; D011201:Poultry Diseases / Q000821:virology; D011213:Poxviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D046370:Sparrows / Q000821:virology*; D014774:Virulence
D004279:DNA, Viral