[Effects of pentylenetetrazol at subconvulsant and convulsant dose on NMDA receptor subunits in rats].
To investigate the expression of N-methyl-D-asparate (NMDA) receptor subunit proteins after administration of different doses of pentylenetetrazol (PTZ).



After ip injection of a subconvulsant (35 mg/kg) and convulsant (50 mg/kg) dose of PTZ, the rats were decapitated at different time points. The levels of cortical NR1  NR2A and NR2B subunit proteins were detected by immunoblotting.



35 mg/kg PTZ and 50 mg/kg PTZ elicited different behavioral changes (P<0.001). The NR2A subunit in the cortex significantly increased 1 h after PTZ injection (P<0.05). For the 50 mg/kg group, both the NR2A and NR2B subunits proteins increased at 1 h in the cortex and then decreased; the protein levels returned to normal after 48 h. However, NR1 subunit had no changes.



The NR2 subunit is involved in PTZ-induced seizure.
Zhu
Li-jun
LJ
Department of Neurobiology, College of Medicine, Zhejiang University, Hangzhou 310031, China.
Wang
Min-zhen
MZ
Wu
Xiao-hua
XH
Xu
Shu-jun
SJ
Luo
Jian-hong
JH
Chen
Zhong
Z
D000818:Animals; D002540:Cerebral Cortex / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D008297:Male; D010433:Pentylenetetrazole / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000032:analysis*; D012640:Seizures / Q000139:chemically induced* / Q000378:metabolism
D010433:Pentylenetetrazole; D016194:Receptors, N-Methyl-D-Aspartate; C409466:NR1 NMDA receptor; C121001:NR2B NMDA receptor