title: Leptin receptor expression on T lymphocytes modulates chronic intestinal inflammation in mice.
abstract: BACKGROUND
  Leptin regulates appetite through the long isoform of its receptor in the hypothalamus. Although leptin regulates immune responses, it is still unknown whether a direct effect of leptin on lymphocytes is required.
  
  
  OBJECTIVE
  To clarify whether expression of leptin receptors on T lymphocytes modulates intestinal inflammation in mice.
  
  
  METHODS
  The model of colitis induced by transfer of CD4(+)CD45RB(high) (RB(high)) cells into scid mice was used. Wild-type (WT) or leptin receptor deficient (db/db) RB(high) cells were transferred into scid mice and development of colitis evaluated.
  
  
  RESULTS
  Leptin receptors were expressed on both RB(high) and RB(low) cells. Intestinal lymphocytes of mice with colitis expressed high leptin levels compared with healthy controls whereas the opposite was true for serum leptin levels. Transfer of RB(high) cells from db/db mice induced delayed disease compared with transfer of WT cells. A high rate of apoptosis in lamina propria lymphocytes and reduced cytokine production were observed early on in scid mice receiving db/db RB(high) cells. These effects were not due to the high levels of glucocorticoids present in db/db mice as administration of corticosterone to WT mice failed to reproduce this phenomenon. High expression of peroxisome proliferator activated receptor gamma was observed in the colon of recipients of db/db compared with WT cells. Freshly isolated db/db RB(high) cells produced low levels of interferon gamma. Despite delayed onset of colitis, as disease progressed differences between mice receiving WT or db/db cells were no longer apparent.
  
  
  CONCLUSIONS
  These results suggest that leptin affects the immune response, partly by acting on the long isoform of its receptor expressed on T lymphocytes.
authors.affiliation: Charite Universitaetsmedizin Berlin, Campus Benjamin Franklin, Medizinische Klinik I, Hindenburgdamm 30, 12200 Berlin, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Siegmund
authors.forename: J A
authors.initials: JA
authors.lastname: Sennello
authors.forename: J
authors.initials: J
authors.lastname: Jones-Carson
authors.forename: F
authors.initials: F
authors.lastname: Gamboni-Robertson
authors.forename: H A
authors.initials: HA
authors.lastname: Lehr
authors.forename: A
authors.initials: A
authors.lastname: Batra
authors.forename: I
authors.initials: I
authors.lastname: Fedke
authors.forename: M
authors.initials: M
authors.lastname: Zeitz
authors.forename: G
authors.initials: G
authors.lastname: Fantuzzi
mesh_terms: D000818:Animals; D002908:Chronic Disease; D003092:Colitis / Q000276:immunology*; D003345:Corticosterone / Q000097:blood; D016207:Cytokines / Q000096:biosynthesis; D018450:Disease Progression; D004198:Disease Susceptibility; D018928:Immunity, Mucosal; D020738:Leptin / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000032:analysis; D017710:Lymphocyte Transfusion; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D011956:Receptors, Cell Surface / Q000276:immunology*; D054411:Receptors, Leptin; D013601:T-Lymphocytes / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology
chemical_list: D016207:Cytokines; D020738:Leptin; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; C515447:leptin receptor, mouse; D017493:Leukocyte Common Antigens; D003345:Corticosterone