title: [Study on the immunogenicity of HIV-1 gag vaccine].
abstract: OBJECTIVE
  To detect the immunogenicity of HIV-1 gag DNA vaccine.
  
  
  METHODS
  The serum antibody titers of the immunized mice were detected by ELISA. The numbers of spleen's CD4(+) and CD8(+) T cells were analyzed by the fluorescent antibody staining and the cytotoxic activity was determined by lactate dehydrogenase (LDH) release assay.
  
  
  RESULTS
  The titers of the serum antibodies and the numbers and cytotoxic activity of spleen's T lymphocyte subsets had significant differences (P<0.05 and P<0.01, respectively) between pVAXGAG and pVAX1 vaccinated groups.
  
  
  CONCLUSIONS
  HIV-1 DNA vaccine plasmid pVAXGAG could elicit both the specific humoral and cellular immunities in BALB/c mice.
authors.affiliation: Key Laboratory of Genetic Engineering, Quartermaster University of PLA, Changchun 130062, China. wzjiang@hotmail.com
authors.forename: Wen-Zheng
authors.initials: WZ
authors.lastname: Jiang
authors.forename: Ning-Yi
authors.initials: NY
authors.lastname: Jin
authors.forename: Zi-Jian
authors.initials: ZJ
authors.lastname: Li
authors.forename: Li-Shu
authors.initials: LS
authors.lastname: Zhang
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D000818:Animals; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005260:Female; D015683:Gene Products, gag / Q000276:immunology*; D015483:HIV Antibodies / Q000032:analysis; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D018655:Lymphocyte Count; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D015683:Gene Products, gag; D015483:HIV Antibodies; D019444:Vaccines, DNA