title: Enhanced immunodetection of cell-free synthesized erythropoietin under denaturing conditions.
abstract: A monoclonal antibody produced by hydridoma cell line, ATCC HB8209, was used to detect and purify erythropoietin synthesized in a cell-free system. The antibody was raised against the N-terminal 20 residues of erythropoietin. It retained anti-erythropoietin activity in 6 M urea in which most of the cell-free synthesized erythropoietin became soluble and gave an enhanced activity of the antibody.
authors.affiliation: School of Chemical Engineering, College of Engineering, Seoul National University, Seoul 151-742, Korea.
authors.forename: Taek Jin
authors.initials: TJ
authors.lastname: Kang
authors.forename: Hui Kyoung
authors.initials: HK
authors.lastname: Song
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Ahn
authors.forename: Tae Wan
authors.initials: TW
authors.lastname: Kim
authors.forename: Yeo Joon
authors.initials: YJ
authors.lastname: Yoon
authors.forename: Cha Yong
authors.initials: CY
authors.lastname: Choi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002474:Cell-Free System / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004921:Erythropoietin / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D011489:Protein Denaturation
chemical_list: D000911:Antibodies, Monoclonal; D004921:Erythropoietin