[Separation of high-quality sperm by PureSperm centrifugation applied to intrauterine insemination cycles].
OBJECTIVE
To separate high-quality sperm by PureSperm centrifugation applied to intrauterine insemination (IUI) cycles.


METHODS
We compared the separate results after washing the semen with one-layer and two-layer PureSperm gradient centrifugation methods and two-layer Percoll gradient centrifugation method, and used the recovered high-quality sperm for IUI.


RESULTS
The density of the sperm washed with one-layer PureSperm centrifugation method was significantly higher than that washed with two-layer Percoll and two-layer PureSperm centrifugation methods(P < 0.01), but there were no differences in all the results between the use of two-layer Percoll and two-layer PureSperm(P > 0.05). No significant differences in the motility, teratozoospermia and IUI results were found when the three methods were used for sperm preparation(P > 0.05). The percentage of morphologically normal sperm was markedly increased, and the non-sperm components such as leucocytes, epithelial cells and cellular fragments were significantly reduced after washed by the two methods.


CONCLUSIONS
PureSperm centrifugation is a safe, efficient and easy method for separating high-quality sperm on intrauterine insemination cycles.
Zhang
Yan
Y
Human Clinical Reproductive Medicine Center, Department of Obstetrics & Gynecology, First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Qian
Yun
Y
Feng
Ting
T
Cai
Lingbo
L
Liu
Jiayin
J
D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D005260:Female; D006801:Humans; D007315:Insemination, Artificial; D008297:Male; D013094:Spermatozoa / Q000166:cytology*