title: Improved extension of platelet storage in a polyolefin container with higher oxygen permeability.
abstract: This study evaluated a newly developed polyolefin bag, which has a 50%/m(2) higher oxygen permeability to extend the shelf-life of platelets. Single-donor aphaeresis platelets were pooled, separated equally into two bags, PO-80 (0.8 l capacity) and KBP-PO (1 l capacity) for control, and stored in plasma for up to 7 d. Platelet biochemical and functional parameters were monitored in bags containing high (4.2 x 10(11)/250 ml/bag, n = 9) and low (2.0 x 10(11)/200 ml/bag, n = 3) concentrations of platelets over the storage period. After 7 d of storage, the PO-80 bags containing high concentrations of platelets had a better pH (mean pH 6.74) than those stored in KBP-PO (pH 6.32, P < 0.01); none of the nine PO-80 bags with a high platelet concentration had a pH below 6.2, compared with four of nine controls (P < 0.05). Similarly, lactate values were 20.19 mmol/l and 28.09 mmol/l respectively (P < 0.05). Aerobic metabolism was maintained better with greater O(2) consumption and less lactate generation in high-platelet concentration PO-80 bags than in the control bags. A significant difference was also found in pH, pCO(2) and lactate levels between the two bags containing low concentrations of platelets during the 7-d period. The in vitro characteristics of platelets declined less over 7 d when stored in a higher oxygen permeable container than in a marketed bag.
authors.affiliation: Division of Blood Transfusion and Transplantation Immunology, Fukushima Medical University School of Medicine, Fukushima, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yuasa
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ohto
authors.forename: Reiko
authors.initials: R
authors.lastname: Yasunaga
authors.forename: Takanori
authors.initials: T
authors.lastname: Kai
authors.forename: Noriaki
authors.initials: N
authors.lastname: Shirahama
authors.forename: Takashi
authors.initials: T
authors.lastname: Ogata
mesh_terms: D001792:Blood Platelets / Q000378:metabolism; D001793:Blood Preservation / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010979:Platelet Function Tests; D011090:Polyenes
chemical_list: D007773:Lactates; D011090:Polyenes; C035051:PL 732; D010100:Oxygen