title: Discovery of the chemical function of glycosidases: design, synthesis, and evaluation of mass-differentiated carbohydrate libraries.
abstract: [reaction: see text] Discovery of the catalytic chemical function of the many putative glycosidases coded in genomes currently relies on individual testing of possible substrates, usually as their p-nitrophenol conjugate. Herein, we present an alternative chemical proteomics approach using a synthetic mass-differentiated heat-stable substrate library with mass spectrometry readout. Library components do not serve as reaction inhibitors and both primary and secondary enzyme substrates can be delineated.
authors.affiliation: Department of Chemistry and the Plant Sciences Institute, Gilman Hall, Iowa State University, Ames, Iowa 50011-3111, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Kwang-Seuk
authors.initials: KS
authors.lastname: Ko
authors.forename: Corbin J
authors.initials: CJ
authors.lastname: Zea
authors.forename: Nicola L
authors.initials: NL
authors.lastname: Pohl
mesh_terms: D002241:Carbohydrates / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry; D002384:Catalysis; D020650:Combinatorial Chemistry Techniques; D005069:Evaluation Studies as Topic; D006026:Glycoside Hydrolases / Q000032:analysis / Q000737:chemistry; D020027:Pyrococcus furiosus / Q000201:enzymology; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D002241:Carbohydrates; D006026:Glycoside Hydrolases