Improved method for the isolation of RNA from (standing liquid cultures of) Streptomycetes.
Streptomycetes are complex soil bacteria capable of producing aerial reproductive mycelium and secondary metabolites. We observed novel phenomena such as an extended life cycle including flotation and anaerobiosis using standing liquid cultures. This paper describes an improved method for isolating good quality RNA from standing liquid cultures of S. coelicolor via excellent cell lysis.
van Keulen
Geertje
G
Department of Microbiology, Groningen Biotechnology and Biomolecular Science Institute (GBB), University of Groningen, PO Box 14, 9750 AA Haren, The Netherlands. g.van.Keulen@biol.rug.nl
Siebring
Jeroen
J
Rembacz
Krzysztof P
KP
Hoogeveen
Martin
M
Tomczynska
Magdalena
M
Dijkhuizen
Lubbert
L
D015152:Blotting, Northern; D003850:Deoxyribonuclease I / Q000737:chemistry; D006146:Guanidines / Q000737:chemistry; D017879:Isothiocyanates / Q000737:chemistry; D019800:Phenol / Q000737:chemistry; D012329:RNA, Bacterial / Q000302:isolation & purification*; D013171:Spores, Bacterial; D013302:Streptomyces / Q000737:chemistry / Q000235:genetics*
D006146:Guanidines; D017879:Isothiocyanates; D012329:RNA, Bacterial; C054435:guanidine isothiocyanate; D019800:Phenol; D003850:Deoxyribonuclease I