Progesterone determination in Iberian red deer by time-resolved fluorometry: an alternative method to RIA.
The validation for Iberian red deer of a commercially available Time-resolved fluoroimmunoassay (TR-FIA) designed for analysis of progesterone in human beings was carried out. Intra-assay coefficients of variation ranged from 3.6% to 7.4%, while inter-assay coefficients of variation varied from 5.2% to 15.5%. Accuracy, evaluated by comparing results yielded by TR-FIA with those obtained from a validated radioimmunoassay (RIA) in the measurement of 14 samples, provided a high regression coefficient (R(2)= 0.93). Different progesterone concentrations added to pool plasma showed percentages of recovery that ranged between 102.6% and 82.48%. The limit of detection was 0.102 nmol/L. The results obtained indicate that the present method is suitable for the measurement of progesterone in female Iberian red deer.
Parra
María Dolores
MD
Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Murcia, 30100 Murcia, Spain.
García
Andrés José
AJ
Bernal
Luis Jesús
LJ
Landete-Castillejos
Tomás
T
Cerón
José Joaquín
JJ
D000818:Animals; D003670:Deer / Q000097:blood*; D005260:Female; D005470:Fluorometry / Q000379:methods; D011374:Progesterone / Q000097:blood*; D011863:Radioimmunoassay; D012044:Regression Analysis; D015203:Reproducibility of Results; D013030:Spain; D013997:Time Factors
D011374:Progesterone