title: Chromatin from two classes of platyhelminthes display both protist H1 and higher eukaryote core histones.
abstract: Histones from the parasitic platyhelminthes, Echinococcus granulosus and Fasciola hepatica, were systematically characterized. Core histones H2A, H2B, H3 and H4, which were identified on the basis of amino acid sequencing and mass spectrometry data, showed conserved electrophoretic patterns. Histones H1, identified on the basis of physicochemical properties, amino acid composition and amino acid sequencing, showed divergence, both in their number and electrophoretic mobilities, between the two species and among other organisms. According to these data, core histones but not H1 histones, would be stabilized during evolution at the level of platyhelminthes.
authors.affiliation: Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Casilla 70061, Correo 7, Santiago, Chile.
authors.forename: Mario
authors.initials: M
authors.lastname: Galindo
authors.forename: Nelson
authors.initials: N
authors.lastname: Varela
authors.forename: Ingrid
authors.initials: I
authors.lastname: Espinoza
authors.forename: Gabriela Cecilia
authors.initials: GC
authors.lastname: Toro
authors.forename: Ulf
authors.initials: U
authors.lastname: Hellman
authors.forename: Christer
authors.initials: C
authors.lastname: Wernstedt
authors.forename: Norbel
authors.initials: N
authors.lastname: Galanti
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002843:Chromatin / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004446:Echinococcus / Q000737:chemistry* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005210:Fasciola hepatica / Q000737:chemistry* / Q000378:metabolism; D006657:Histones / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012617:Sea Urchins / Q000737:chemistry / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013950:Thymus Gland / Q000737:chemistry / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000737:chemistry / Q000378:metabolism
chemical_list: D000596:Amino Acids; D002843:Chromatin; D006657:Histones