title: [Construction and expression of the fusion vector of His-tagged human ARPC2 gene].
abstract: OBJECTIVE
  To construct the expression vector of His-ARPC2 fusion protein and obtain its expression and purification in E. coli.
  
  
  METHODS
  ARPC2 cDNA codon region was amplified by PCR from human liver cDNA library and cloned into pET-14b vector following the routine procedures. After identification by enzyme digestion, PCR and sequencing, the positive clones were transformed into BL21 (DE3) competent cells, and the expression of His-ARPC2 fusion protein was induced with IPTG and further purified by Ni-NTA affinity chromatography.
  
  
  RESULTS
  The constructed His-ARPC2 fusion protein vector was highly efficiently expressed in E. coli. With Ni-NTA affinity chromatography, a purified His fusion protein with relative molecular mass of approximately 36 000 was obtained.
  
  
  CONCLUSIONS
  The expression vector of His-ARPC2 fusion protein is constructed, expressed and purified under non-denaturing conditions, which may significantly facilitate future study of the physiological functions of ARPC2 and characterization of its interaction proteins.
authors.affiliation: Department of Pathophysiology and Key Laboratory of Shock and Microcirculation of PLA, First Military Medical University, Guangzhou 510515, China.
authors.forename: Xiao-wei
authors.initials: XW
authors.lastname: Gong
authors.forename: Yi
authors.initials: Y
authors.lastname: Peng
authors.forename: Jing-hua
authors.initials: JH
authors.lastname: Liu
authors.forename: Zhi-jie
authors.initials: ZJ
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Deng
authors.forename: Qing-he
authors.initials: QH
authors.lastname: Qin
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D051377:Actin-Related Protein 2; D051378:Actin-Related Protein 3; D003598:Cytoskeletal Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins / Q000235:genetics*
chemical_list: C495926:ACTR2 protein, human; C495928:ACTR3 protein, human; D051377:Actin-Related Protein 2; D051378:Actin-Related Protein 3; D003598:Cytoskeletal Proteins; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins