title: Simultaneous identification of rifampin-resistant Mycobacterium tuberculosis and nontuberculous mycobacteria by polymerase chain reaction-single strand conformation polymorphism and sequence analysis of the RNA polymerase gene (rpoB).
abstract: Interspecies variations and mutations associated with rifampin resistance in rpoB of Mycobacterium allow for the simultaneous identification of rifampin-resistant Mycobacterium tuberculosis and nontuberculous mycobacteria by PCR-SSCP analysis and PCR- sequencing. One hundred and ten strains of rifampin-susceptible M. tuberculosis, 14 strains of rifampin-resistant M. tuberculosis, and four strains of the M. avium complex were easily identified by PCR-SSCP. Of another seven strains, which showed unique SSCP patterns, three were identified as rifampin-resistant M. tuberculosis and four as M. terrae complex by subsequent sequence analysis of their rpoB DNAs (306 bp). These results were concordant with those obtained by susceptibility testing, biochemical identification, and 16S rDNA sequencing.
authors.affiliation: Department of Microbiology and Cancer Research Institute, Institute of Endemic Diseases, SNUMRC, Seoul National University College of Medicine, and Clinical Research Institute, Seoul National University Hospital, Chongno-Gu, South Korea.
authors.forename: Bum-Joon
authors.initials: BJ
authors.lastname: Kim
authors.forename: Keun-Hwa
authors.initials: KH
authors.lastname: Lee
authors.forename: Yeo-Jun
authors.initials: YJ
authors.lastname: Yun
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Park
authors.forename: Young-Gil
authors.initials: YG
authors.lastname: Park
authors.forename: Gil-Han
authors.initials: GH
authors.lastname: Bai
authors.forename: Chang-Yong
authors.initials: CY
authors.lastname: Cha
authors.forename: Yoon-Hoh
authors.initials: YH
authors.lastname: Kook
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D008969:Molecular Sequence Data; D015269:Mycobacterium avium Complex / Q000145:classification / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012293:Rifampin / Q000494:pharmacology*; D016415:Sequence Alignment; D014376:Tuberculosis / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents; D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D012293:Rifampin