title: Establishment of a pure culture of the hitherto uncultured unicellular cyanobacterium Aphanothece sacrum, and phylogenetic position of the organism.
abstract: Aphanothece sacrum, an edible freshwater unicellular cyanobacterium, was isolated by using novel synthetic media (designated AST and AST-5xNP). The media were designed on the basis of the ratio of inorganic elements contained in A. sacrum cells cultured in a natural pond. The isolated strain exhibits unicellular rod-shaped cells approximately 6 microm in length that are scattered in an exopolysaccharide matrix, a feature similar to that of natural A. sacrum. DNA analysis of the isolated strain revealed that it carried two ferredoxin genes whose deduced amino acid sequences were almost identical to previously published sequences of ferredoxins from natural A. sacrum. Analysis of the 16S rRNA gene and ferredoxin genes revealed that A. sacrum occupies a phylogenetically unique position among the cyanobacteria.
authors.affiliation: Hagiwara Institute of Health, Kasai, Hyogo 679-0105, Japan. fujishiro@hih.gr.jp
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Fujishiro
authors.forename: Takahira
authors.initials: T
authors.lastname: Ogawa
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Matsuoka
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nagahama
authors.forename: Yasunobu
authors.initials: Y
authors.lastname: Takeshima
authors.forename: Hideaki
authors.initials: H
authors.lastname: Hagiwara
mesh_terms: D001431:Bacteriological Techniques; D001483:Base Sequence; D003470:Culture Media; D000458:Cyanobacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000032:analysis; D005288:Ferredoxins / Q000737:chemistry / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D003470:Culture Media; D004275:DNA, Ribosomal; D005288:Ferredoxins; D012336:RNA, Ribosomal, 16S