title: Effects of high static magnetic field exposure on different DNAs.
abstract: The effects of magnetic fields produced by permanent magnets on different DNA sources were investigated in vivo and in vitro. Escherichia coli DNA, plasmid, and amplification products of different lengths were used as the magnetic field target. The in vivo assays did not reveal any DNA alterations following exposure, demonstrating the presence of cell dependent mechanisms, such as the repair system and the buffering action of the heat shock proteins DNA K/J (Hsp 70/40). The in vitro assays displayed interactions between the magnetic field and DNA, revealing principally that magnetic field exposure induces DNA alterations in terms of point mutations. We speculate that the magnetic field can perturb DNA stability interacting with DNA directly or potentiating the activity of oxidant radicals. This genotoxic effect of the magnetic field, however, is minimized in living organisms due to the presence of protective cellular responses.
authors.affiliation: Istituto di Chimica Biologica "Giorgio Fornaini", Università Degli Studi di Urbino, Urbino (PU), Italy. l.potenza@uniurb.it
authors.forename: Lucia
authors.initials: L
authors.lastname: Potenza
authors.forename: Luigi
authors.initials: L
authors.lastname: Cucchiarini
authors.forename: Elena
authors.initials: E
authors.lastname: Piatti
authors.forename: Umberta
authors.initials: U
authors.lastname: Angelini
authors.forename: Marina
authors.initials: M
authors.lastname: Dachà
mesh_terms: D002021:Buffers; D019610:Cytoprotection; D004247:DNA / Q000187:drug effects / Q000528:radiation effects*; D004260:DNA Repair; D004269:DNA, Bacterial / Q000187:drug effects / Q000528:radiation effects; D004926:Escherichia coli / Q000235:genetics; D005609:Free Radicals / Q000494:pharmacology; D018840:HSP70 Heat-Shock Proteins / Q000502:physiology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008280:Magnetics; D018832:Molecular Chaperones / Q000502:physiology; D016877:Oxidants / Q000494:pharmacology; D010957:Plasmids / Q000187:drug effects / Q000528:radiation effects; D017354:Point Mutation / Q000528:radiation effects; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA
chemical_list: D002021:Buffers; D004269:DNA, Bacterial; D005609:Free Radicals; D018840:HSP70 Heat-Shock Proteins; D018832:Molecular Chaperones; D016877:Oxidants; D004247:DNA; D006861:Hydrogen Peroxide