Purification and characterization of liver lectins from a lizard, Sceloporus spinosus.
This study discusses the purification of soluble beta-galactose lectins obtained from the lizard liver of Sceloporus spinosus. The first lectin named lizard hepatic lectin-1 (LHL-1) presented a molecular weight of 31,750, with an isoelectric point of 4.25. The highest specific hemagglutinating activity was achieved using human blood type A1: N-acetylgalactosamine (GalNAc)-galactose (Gal)-fucose (Fuc). Carbohydrate inhibition assays indicated a higher lectin specificity for GalNAc. For LHL-2 the molecular weight obtained was 23,850 with an isoelectric point of 3.25. The highest carbohydrate specificity was observed for Gal. These lizard hepatic lectins are similar to the mammal hepatic lectins previously reported. However, it is different from the alligator hepatic lectin (AHL). The homology analyses of LHL-1 resulted in 100% identity with the Steroidogenic acute regulatory protein (StAR), while LHL-2 was similar to adenylate kinase (75% identity). We suggest that these liver lectins are related to the inherent functions of liver previously reported.
Fenton
N Bertha
NB
División de Estudios de Posgrado, Facultad de Medicina Dr. Ignacio Chávez UMSNH, Dr. Rafael Carrillo Esq., Dr. Salvador Gonzales Herrejón, Morelia, Michoacán, México. betha00_mx@yahoo.com
Arreguín
L Barbarin
LB
Méndez
C Fausto
CF
Arreguín
E Roberto
ER
D000017:ABO Blood-Group System / Q000737:chemistry; D000116:Acetylgalactosamine / Q000737:chemistry; D000371:Agglutination; D000595:Amino Acid Sequence; D000818:Animals; D001769:Blood; D005643:Fucose / Q000737:chemistry; D005690:Galactose / Q000737:chemistry; D006801:Humans; D037102:Lectins / Q000737:chemistry* / Q000302:isolation & purification*; D008099:Liver / Q000737:chemistry*; D008116:Lizards; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid
D000017:ABO Blood-Group System; D037102:Lectins; D005643:Fucose; D000116:Acetylgalactosamine; D005690:Galactose