Gene cataloging and expression profiling in human gastric cancer cells by expressed sequence tags.
To understand the molecular mechanism associated with gastric carcinogenesis, we identified genes expressed in gastric cancer cell lines and tissues. Of 97,609 high-quality ESTs sequenced from 36 cDNA libraries, 92,545 were coalesced into 10,418 human Unigene clusters (Build 151). The gene expression profile was produced by counting the cluster frequencies in each library. Although the profiles of highly expressed genes varied greatly from library to library, those genes related to cell structure formation, heat shock proteins, the glycolysis pathway, and the signaling pathway were highly represented in human gastric cancer cell lines and in primary tumors. Conversely, the genes encoding immunoglobulins, ribosomal proteins, and digestive proteins were down-regulated in gastric cancer cell lines and tissues compared to normal tissues. The transcription levels of some of these genes were confirmed by RT-PCR. We found that genes related to cell adhesion, apoptosis, and cytoskeleton formation were particularly up-regulated in the gastric cancer cell lines established from malignant ascites compared to those from primary tumors. This comprehensive molecular profiling of human gastric cancer should be useful for elucidating the genetic events associated with human gastric cancer.
Kim
Nam-Soon
NS
Laboratory of Human Genomics, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, South Korea.
Hahn
Yoonsoo
Y
Oh
Jung-Hwa
JH
Lee
Ju-Yeon
JY
Oh
Kyung-Jin
KJ
Kim
Jeong-Min
JM
Park
Hong-Seog
HS
Kim
Sangsoo
S
Song
Kyu-Sang
KS
Rho
Seung-Moo
SM
Yoo
Hyang-Sook
HS
Kim
Yong Sung
YS
D001483:Base Sequence; D045744:Cell Line, Tumor; D019295:Computational Biology; D015536:Down-Regulation / Q000235:genetics; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D013274:Stomach Neoplasms / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics