title: Changes in cytokine production and impaired hematopoiesis in patients with anorexia nervosa: the effect of refeeding.
abstract: The changes in cytokines and hormones involved in hematopoiesis were studied in the serum of 7 girls with anorexia nervosa, 15-24 yr old, on admission and after 5% and 10% weight gain. Hematopoiesis was studied by in-vitro culturing of circulating granulocyte-macrophage colony forming cells and erythroid burst forming cells. Nutritional status was studied by anthropometric measurements and resting energy expenditure. On admission, granulocyte-macrophage colony forming cells and erythroid burst forming cells were significantly lower than in age-matched controls and increased significantly along weight gain. Blood leptin and erythropoietin levels increased significantly with weight gain. TNF-alpha levels tended to decrease while IL-1beta levels were lower than in the controls on admission (p <0.05) and did not change significantly during weight gain. IL-3, GM-CSF and IL-6 were undetected on admission or along weight gain. The changes in granulocyte-macrophage colony forming cells and erythroid burst forming cells positively correlated with changes in resting energy expenditure and fat free mass. These results may suggest that undernutrition affects hematopoiesis as indicated by the reduction of hematopoietic progenitor cells before treatment and the significant increase with weight gain. The changes in the levels of hormones and cytokines known to be involved in hematopoiesis along refeeding may suggest a role for these factors in anorexia nervosa.
authors.affiliation: Clinical Nutrition Unit, Sourasky Tel Aviv Medical Center, 6 Weizmann Street, Tel-Aviv 64239, Israel. vaisman@tasmc.health.gov.il
authors.forename: Nachum
authors.initials: N
authors.lastname: Vaisman
authors.forename: Talia
authors.initials: T
authors.lastname: Hahn
authors.forename: Yocheved
authors.initials: Y
authors.lastname: Karov
authors.forename: Erica
authors.initials: E
authors.lastname: Sigler
authors.forename: Yigal
authors.initials: Y
authors.lastname: Barak
authors.forename: Vivian
authors.initials: V
authors.lastname: Barak
mesh_terms: D000293:Adolescent; D000328:Adult; D000856:Anorexia Nervosa / Q000097:blood* / Q000178:diet therapy* / Q000503:physiopathology; D001835:Body Weight; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood*; D004921:Erythropoietin / Q000097:blood; D005260:Female; D006410:Hematopoiesis; D006728:Hormones / Q000097:blood; D006801:Humans; D020738:Leptin / Q000097:blood; D009752:Nutritional Status
chemical_list: D016207:Cytokines; D006728:Hormones; D020738:Leptin; D004921:Erythropoietin