title: Ultrasensitive ATP detection using firefly luciferase entrapped in sugar-modified sol-gel-derived silica.
abstract: Firefly luciferase (FL) was entrapped in sol-gel-derived silica containing precursors based on covalent linkage of d-gluconolactone or d-maltonolactone to (aminopropyl)triethoxysilane to form N-(3-triethoxysilylpropyl)gluconamide or N-(3-triethoxysilylpropyl)maltonamide. The enzyme was active and stable in this material and showed catalytic constants close to those in solution. As little as 20 amol ATP could be detected with the entrapped FL, and the entrapped enzyme could be used over several cycles.
authors.affiliation: Department of Chemistry, McMaster University, 1280 Main Street West, Hamilton ON, Canada L8S 4M1.
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Cruz-Aguado
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Nadine H
authors.initials: NH
authors.lastname: Elowe
authors.forename: Michael A
authors.initials: MA
authors.lastname: Brook
authors.forename: John D
authors.initials: JD
authors.lastname: Brennan
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D002241:Carbohydrates / Q000737:chemistry*; D002384:Catalysis; D001517:Coleoptera / Q000201:enzymology*; D005782:Gels / Q000737:chemistry*; D008156:Luciferases / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D002241:Carbohydrates; D005782:Gels; D012822:Silicon Dioxide; D000255:Adenosine Triphosphate; D008156:Luciferases