[Effect of Astragalus polysaccharides in promoting neutrophil-vascular endothelial cell adhesion and expression of related adhesive molecules].
OBJECTIVE
To explore the detoxication and tissue generation effect of Astragalus (As) in wound healing and its relation with inflammatory reaction, through observing the effect of Astragalus polysaccharides (AP) on neutrophil-endothelial cell adhesion and expression of related adhesive molecules.


METHODS
Human polymorphonuclear leucocyte (PMN) or human umbilical vein endothelial cell (HUVEC) was treated separately with AP, AP plus interleukin 1 (IL-1) and tumor necrosis factor (TNF) to study the effect of AP on PMN adhesion with HUVEC by rose bengal staining, and that on expression of superficial adhesive factor by means of Cell-ELISA and APAAP method.


RESULTS
When AP acted on HUVEC, it could significantly promote the adhesion of HUVEC with PMN, while when AP acted on PMN, the adhesion would not increase. When HUVEC was treated by AP plus IL-1, the IL-1 induced PMN adhesion with HUVEC could be strengthened, and the expression of HUVEC superficial adhesive factor ICAM-1 induced by IL-1 and TNF was strengthened also, but when PMN treated with AP, it showed no effect on the expression of adhesive factor CD18.


CONCLUSIONS
AP promotes the adhesion between neutrophil and endothelial cell by way of promoting the expression of superficial I-CAM-1 on surface of endothelial cells, so as to improve the inflammatory reaction in the wound healing course, it possibly is one of the biological bases of the detoxication and tissue generation effects of AP.
Hao
Yu
Y
Department of Immunology, Beijing University of CM, Beijing 100029.
Qiu
Quan-ying
QY
Wu
Jun
J
D027885:Astragalus propinquus / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D007375:Interleukin-1 / Q000096:biosynthesis; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology; D014945:Wound Healing / Q000187:drug effects
D007375:Interleukin-1; D011134:Polysaccharides; D018799:Intercellular Adhesion Molecule-1