Cloning of cytochrome P450 1A (CYP1A) genes from the hermaphrodite fish Rivulus marmoratus and the Japanese medaka Oryzias latipes.
To use two small fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae) and the Japanese medaka Oryzias latipes (Belloniformes) as testing models in molecular ecotoxicology, we have cloned the cytochrome P450 1A (CYP1A) gene after screening of both genomic DNA libraries, and sequenced 11,863 and 7,243 bp including all the exons and introns with promoter regions, respectively. The Rivulus and the medaka CYP1A gene consisted of seven exons (including non-coding exons) with high homology to mammals. In the promoter region, Rivulus CYP1A gene has seven xenobiotic response elements (XREs) and two metal response elements (MREs), while the Japanese medaka CYP1A gene has six XREs and four MREs. Interestingly, medaka CYP1A gene has a number of MREs at the promoter, which may affect its response on metal exposure. We describe here the gene structure of both fish CYP1A genes.
Kim
Il-Chan
IC
Department of Biochemistry, College of Medicine, Hanyang University, Seoul 133-791, Republic of Korea.
Kim
Young Ja
YJ
Yoon
Yong-Dal
YD
Kawamura
Shoji
S
Lee
Yong-Sung
YS
Lee
Jae-Seong
JS
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016000:Cluster Analysis; D003532:Cyprinodontiformes / Q000235:genetics*; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D040461:Gene Components; D008969:Molecular Sequence Data; D009990:Oryzias / Q000235:genetics*; D010802:Phylogeny; D020218:Response Elements / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D015262:Xenobiotics
D015262:Xenobiotics; D019363:Cytochrome P-450 CYP1A1