title: B[a]P-DNA binding in early life-stages of Atlantic tomcod: population differences and chromium modulation.
abstract: Atlantic tomcod (Microgadus tomcod) from the Hudson River (HR) are resistant at the molecular and organismic levels to the effects of exposure to dioxin-like aromatic hydrocarbon (AH) compounds, but much less so to benzo[a]pyrene (B[a]P). The aims of this study were to determine in early life-stages of tomcod exposed to B[a]P: (1) if DNA binding levels differed between fish from the HR and Miramichi River (MR), and (2) if co-exposure to chromium could modulate this genotoxic effect. After exposure to [(3)H]B[a]P alone, DNA-bound radioactivity was 5-10-fold higher in embryos and larvae of MR than HR descent. Co-exposure to chromium modulated DNA binding levels in offspring of both populations. In MR embryos, co-exposure to chromium inhibited B[a]P uptake. These results demonstrated resistance to the genotoxic effects of B[a]P in early life stages of HR tomcod at an ecologically important endpoint and suggest the ability of chromium to modulate AH-induced genotoxicity.
authors.affiliation: Nelson Institute of Environmental Medicine, School of Medicine, New York University, 57 Old Forge Road, Tuxedo, NY 10987, USA.
authors.forename: C
authors.initials: C
authors.lastname: Sorrentino
authors.forename: N K
authors.initials: NK
authors.lastname: Roy
authors.forename: R C
authors.initials: RC
authors.lastname: Chambers
authors.forename: S C
authors.initials: SC
authors.lastname: Courtenay
authors.forename: I
authors.initials: I
authors.lastname: Wirgin
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism*; D002857:Chromium / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005399:Fishes / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D009509:New Brunswick; D009518:New York; D045483:Rivers; D013045:Species Specificity; D014316:Tritium
chemical_list: D002857:Chromium; D014316:Tritium; D001564:Benzo(a)pyrene; D004247:DNA