Leukocyte mitochondrial alterations after cardiac surgery involving cardiopulmonary bypass: clinical correlations.
Cardiac surgery with the use of cardiopulmonary bypass (CPB) is known to initiate systemic inflammatory responses that are associated with immune dysregulations, but the pathomechanisms underlying these changes remain elusive. Mitochondrial transmembrane potential (MTP) is an important determinant of leukocytic functions and viability, and may be altered as a part of the cellular responses to systemic inflammatory insults. Therefore, we examined MTP in three subsets of peripheral leukocytes in 18 patients receiving uncomplicated cardiac surgery involving CPB. The MTP of neutrophils and lymphocytes significantly increased, whereas that of monocytes significantly declined, after the surgery. The alterations in leukocytic MTP were transient, normalizing 3 days to 1 week after the surgery, and were accompanied by transient overproduction of intracellular oxidants, including nitric oxide and superoxide. Despite these perturbations, the viability status of leukocytes remained unaltered. Positive correlations were found between the changes of leukocyte MTP and various clinical parameters, implying that leukocyte mitochondrial alterations are parts of the systemic immune perturbations induced by the bypass surgery.
Kong
Chi-Woon
CW
Division of Critical Care, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan. cwkong@vghtpe.gov.tw
Huang
Cheng-Hsiung
CH
Hsu
Tai-Ger
TG
Tsai
Kelvin K C
KK
Hsu
Chen-Fu
CF
Huang
Mei-Chun
MC
Chen
Lung-Ching
LC
D000368:Aged; D015415:Biomarkers / Q000097:blood; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D002470:Cell Survival; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000473:pathology*; D008297:Male; D008564:Membrane Potentials / Q000502:physiology; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology*; D009569:Nitric Oxide / Q000097:blood / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013903:Thoracic Surgery
D015415:Biomarkers; D013481:Superoxides; D009569:Nitric Oxide