Utility of reverse transcriptase PCR for rapid diagnosis of influenza a virus infection and detection of amantadine-resistant influenza a virus isolates.
A reverse transcriptase PCR was developed to detect 50 or 5,000 RNA copies of influenza A virus per ml in throat swab specimens. The assay was more sensitive than the Directigen Flu A test. The technique was also used to detect amantadine-resistant isolates.
Pachucki
Constance T
CT
Department of Infectious Disease and Department of Laboratory Medicine and Pathology, Edward Hines Jr. Veterans Administration Hospital, 111-P, Hines, IL 60141, USA. constance.pachucki@med.va.gov
Khurshid
M Aijaz
MA
Nawrocki
John
J
D000547:Amantadine / Q000494:pharmacology*; D024882:Drug Resistance, Viral; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis*; D010614:Pharynx / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
D000547:Amantadine