title: Immunolocalization of TASK-3 (KCNK9) to a subset of cortical neurons in the rat CNS.
abstract: Tandem pore domain (2P) K channels constitute the most diverse family of K channels and are responsible for background (leak or baseline) K currents. Of the 15 human 2P K channels, TASK-1, TASK-2, and TASK-3 are uniquely sensitive to physiologic pH changes as well as being inhibited by local anesthetics and activated by volatile anesthetics. In this study polyclonal antibodies selective for TASK-3 have been used to localize its expression in the rat central nervous system (CNS). TASK-3 immunostaining was found in rat cortex, hypothalamus, and hippocampus. Double immunofluorescent studies identified a discrete population of TASK-3 expressing neurons scattered throughout cortex. Using immunogold electron microscopy TASK-3 was identified at the cell surface associated with synapses and within the intracellular synthetic compartments. These results provide a more finely detailed picture of TASK-3 expression and indicate a role for TASK-3 in modulating cerebral synaptic transmission and responses to CNS active drugs.
authors.affiliation: Department of Anesthesia and Perioperative Care, University of California, 513 Parnassus Ave., Room S-261, San Francisco, CA 94143-0542, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Callahan
authors.forename: Dmitriy A
authors.initials: DA
authors.lastname: Labunskiy
authors.forename: Anna
authors.initials: A
authors.lastname: Logvinova
authors.forename: Mona
authors.initials: M
authors.lastname: Abdallah
authors.forename: Canhui
authors.initials: C
authors.lastname: Liu
authors.forename: Joseph F
authors.initials: JF
authors.lastname: Cotten
authors.forename: C Spencer
authors.initials: CS
authors.lastname: Yost
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D006863:Hydrogen-Ion Concentration; D008297:Male; D008854:Microscopy, Electron; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D015221:Potassium Channels / Q000378:metabolism*; D024683:Potassium Channels, Tandem Pore Domain; D051381:Rats; D017207:Rats, Sprague-Dawley; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: C407082:Kcnk9 protein, rat; D015221:Potassium Channels; D024683:Potassium Channels, Tandem Pore Domain