title: Elevated circulating endothelial membrane microparticles in paroxysmal nocturnal haemoglobinuria.
abstract: We analysed endothelial cell membrane microparticles (ECMP) in the peripheral blood of patients with paroxysmal nocturnal haemoglobinuria (PNH) (n = 9), aplastic anaemia (AA) (n = 10), sickle cell disease (SCD) (n = 8), and healthy donors (HD) (n = 11). There was no clinically manifested thrombosis in the PNH or AA group, except one cured thrombophlebitis (PNH), while all SCD patients had a history of vaso-occlusive crises. We used three-colour flow cytometry with blood cell-specific antibodies and antibodies to endothelial antigens CD105 and CD144. Phosphatidylserine-positive microparticles were detected using the annexin V-binding (AVB) assay. The population of CD105+AVB+ ECMP was significantly (P < 0.05) higher in SCD (median: 0.568 x 10(9)/l; 25-75th percentile range: 0.351-0.976 x 10(9)/l) and PNH (0.401 x 10(9)/l; 0.19-0.441 x 10(9)/l) patients when compared with AA (0.122 x 10(9)/l; 0.061-0.172 x 10(9)/l) or HD (0.180 x 10(9)/l; 0.137-0.217 x 10(9)/l) group. Even more pronounced differences were observed in ECMP exhibiting a marker of inflammatory stimulation CD54 (CD105+CD54+). Similarly, ECMP that exhibited endothelial specific and proteolysis-sensitive antigen CD144 were increased in SCD and PNH, but not in AA. Elevated CD54+ ECMP may reflect the inflammatory status of endothelial cells in SCD and PNH, while CD144+ ECMP could indicate continuous endothelial stimulation and/or injury. Analysis of circulating ECMP appears promising to provide useful information on the status of the vascular endothelium in PNH and SCD.
authors.affiliation: Laboratory of Cellular Hematology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD, USA. jan.simak@fda.hhs.gov
authors.forename: Jan
authors.initials: J
authors.lastname: Simak
authors.forename: Karel
authors.initials: K
authors.lastname: Holada
authors.forename: Antonio M
authors.initials: AM
authors.lastname: Risitano
authors.forename: Jan H
authors.initials: JH
authors.lastname: Zivny
authors.forename: Neal S
authors.initials: NS
authors.lastname: Young
authors.forename: Jaroslav G
authors.initials: JG
authors.lastname: Vostal
mesh_terms: D000741:Anemia, Aplastic / Q000097:blood; D000755:Anemia, Sickle Cell / Q000097:blood; D015703:Antigens, CD / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D016022:Case-Control Studies; D002462:Cell Membrane / Q000276:immunology / Q000473:pathology*; D004730:Endothelium, Vascular / Q000276:immunology / Q000473:pathology*; D005434:Flow Cytometry; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood* / Q000276:immunology; D006801:Humans
chemical_list: D015703:Antigens, CD; D015415:Biomarkers