Development of a PCR assay for Streptococcus iniae based on the lactate oxidase (lctO) gene with potential diagnostic value.
Streptococcus iniae is a well-known pathogen of both fish and humans that is difficult to identify by conventional biochemical tests. A PCR assay based on the lactate oxidase (lctO) gene of S. iniae was developed for the rapid and specific detection and identification of this pathogen from different sources. The PCR assay had a detection limit of 62-31 cells, and 25 pg of DNA per PCR reaction mixture. The PCR was also effective in detecting the bacterium from inoculated tissue homogenates, suggesting its potential use for a rapid and accurate diagnosis of S. iniae infections.
Mata
Ana I
AI
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain.
Blanco
M Mar
MM
Domínguez
Lucas
L
Fernández-Garayzábal
José F
JF
Gibello
Alicia
A
D000818:Animals; D017756:Aquaculture; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005393:Fish Diseases / Q000175:diagnosis / Q000382:microbiology*; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000235:genetics*; D017686:Oncorhynchus mykiss; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D021541:Sea Bream; D012680:Sensitivity and Specificity; D013290:Streptococcal Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D013291:Streptococcus / Q000201:enzymology / Q000235:genetics*
D004269:DNA, Bacterial; D006899:Mixed Function Oxygenases; C021497:lactate 2-monooxygenase