Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis.
In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of approximately 49 kDa size, and weak signals for proteins of approximately 41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of approximately 56 and 59 kDa size, and also a weak immunoreaction with a protein of approximately 43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14-40 ng/g DW in controls to 373-638 ng/g DW in exposed mussels.
Jonsson
H
H
Department of Biological Oceanography, Baltic Sea Research Institute, Seestrasse 15, D-18119 Rostock, Germany. henrik.jonsson@io-warnemuende.de
Sandnes
K Viken
KV
Schiedek
D
D
Schneider
R
R
Grøsvik
B E
BE
Goksøyr
A
A
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D049872:Bivalvia / Q000276:immunology*; D015153:Blotting, Western; D020111:Chlorodiphenyl (54% Chlorine) / Q000633:toxicity; D002849:Chromatography, Gas; D017124:Conserved Sequence / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D030562:Databases, Protein; D004790:Enzyme Induction / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D008099:Liver / Q000276:immunology; D008861:Microsomes / Q000276:immunology; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D011078:Polychlorinated Biphenyls / Q000378:metabolism; D016415:Sequence Alignment
D000906:Antibodies; D010446:Peptide Fragments; D020111:Chlorodiphenyl (54% Chlorine); D003577:Cytochrome P-450 Enzyme System; D011078:Polychlorinated Biphenyls