Differences in genetic expression profiles between young-age and old-age gastric adenocarcinoma using cDNA microarray for endocrine disruptor study.
In the current study, we compared the differential expression profiles of genes affected by endocrine disruptor in gastric carcinomas of the young and old using cDNA microarray. Five gastric carcinoma tissues of young patients (< or =35 years) were compared with 5 disease-free gastric tissues of age-matched healthy young people (experiment 1) and 5 gastric carcinoma tissues of old patients (> or =65 years) (experiment 2), respectively. We hybridized the tissues with IntelliGene Human DNA Chip for endocrine disruption study, and identified finally the results common to the two experiments. Of the 220 genes represented on the array, 6 genes were up-regulated and 3 genes were down-regulated in the gastric carcinomas of young patients compared with those of old patients and those of the young volunteers. In conclusion, the genes affected by endocrine disruptors were differently expressed in young-age gastric carcinoma versus the old-age gastric carcinoma. So, we suggest that the gastric carcinoma in the young is the result of a genetic background that differs from that of the old and endocrine disruptors may play an important role in the carcinogenesis of gastric carcinoma in the young.
Chung
Hye Won
HW
Department of Internal Medicine, Institute of Gastroenterology, Yonsei University College of Medicine, Seoul, Korea.
Park
Seung Woo
SW
Chung
Jae Bock
JB
Kang
Jin Kyung
JK
Kim
Ju Won
JW
Kim
Han-Soo
HS
Hyung
Woo Jin
WJ
Noh
Sung Hoon
SH
Song
Si Young
SY
D000230:Adenocarcinoma / Q000235:genetics*; D000328:Adult; D000367:Age Factors; D000368:Aged; D017931:DNA Primers; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics*
D017931:DNA Primers; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm