title: BKV and SV40 infection of human kidney tubular epithelial cells in vitro.
abstract: The interaction of BKV with its natural target cells, human kidney epithelial cells, has not been studied. In vitro infections of human primary kidney epithelial cells were performed to investigate a BKV infection in its natural host cell. BKV undergoes a lytic replication cycle in this system: high levels of T antigen expression were first detected at 36 h postinfection, while viral DNA replication, capsid protein expression, and progeny virus were observed at 48 h postinfection. It was observed that the related polyomavirus SV40 is incapable of infecting human kidney epithelium except in the presence of the GM1 ganglioside, recently reported to be an SV40 receptor.
authors.affiliation: Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Low
authors.forename: H David
authors.initials: HD
authors.lastname: Humes
authors.forename: Mark
authors.initials: M
authors.lastname: Szczypka
authors.forename: Michael
authors.initials: M
authors.lastname: Imperiale
mesh_terms: D000818:Animals; D000957:Antigens, Viral, Tumor / Q000378:metabolism; D001739:BK Virus / Q000472:pathogenicity*; D002478:Cells, Cultured; D004261:DNA Replication; D004847:Epithelial Cells / Q000821:virology*; D006801:Humans; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000821:virology*; D013539:Simian virus 40 / Q000472:pathogenicity*; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D000957:Antigens, Viral, Tumor; D014764:Viral Proteins