title: Effect of intrauterine infusion of Escherichia coli on hormonal patterns in gilts during the oestrous cycle.
abstract: The aim of this study was to determine the effect of intrauterine Escherichia coli infusion on the patterns of plasma LH, prolactin, progesterone, androstenedione, testosterone, oestrone, oestradiol-17beta, cortisol and 13,14-dihydro-15-keto-prostaglandin F2alpha (PGFM) in gilts during the oestrous cycle. On day 4 of the oestrous cycle (day 0), 25 mL of saline or 25 mL of Escherichia coli suspension, containing 10(7) colony forming units x mL(-1), was infused once into the each uterine horn in group I or II respectively. The control gilts developed a new oestrous cycle at the expected time but not bacteria-treated. Endometritis and vaginal discharge developed in all gilts after Escherichia coli infusion. The administration of Escherichia coli resulted in a reduction of plasma levels of LH, prolactin, oestrone and oestradiol-17beta (P < 0.05-0.001), mainly on days 15-18 after treatment (expected perioestrous period). During this time, the plasma androstenedione level was elevated (P < 0.05-0.001) after bacteria infusion. In the gilts receiving bacteria, progesterone concentration decreased from day 8 after treatment and was low until the end of the study (P < 0.05-0.001). On days 8-12 after bacteria administration, the level of PGFM was higher (P < 0.001) than that found in the control group. These results suggest that the developing inflammatory process of the endometrium in gilts following Escherichia coli infusion significantly affects the pituitary-ovarian axis function as well as prostaglandin production leading to anoestrus.
authors.affiliation: Division of Reproductive Endocrinology and Pathophysiology, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, 10-747 Olsztyn, Tuwima 10, Poland. baja@pan.olsztyn.pl
authors.forename: Barbara
authors.initials: B
authors.lastname: Jana
authors.forename: Jan
authors.initials: J
authors.lastname: Kucharski
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Ziecik
mesh_terms: D000735:Androstenedione / Q000097:blood; D000818:Animals; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood; D004716:Endometritis / Q000097:blood / Q000503:physiopathology / Q000662:veterinary*; D004926:Escherichia coli; D004927:Escherichia coli Infections / Q000097:blood / Q000503:physiopathology / Q000662:veterinary*; D004958:Estradiol / Q000097:blood; D004970:Estrone / Q000097:blood; D004971:Estrus / Q000097:blood*; D005260:Female; D006854:Hydrocortisone / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood; D011374:Progesterone / Q000097:blood; D011388:Prolactin / Q000097:blood; D011460:Prostaglandins F / Q000097:blood; D011897:Random Allocation; D013552:Swine / Q000097:blood / Q000502:physiology*; D013553:Swine Diseases / Q000097:blood / Q000503:physiopathology*; D013739:Testosterone / Q000097:blood; D014599:Uterus / Q000378:metabolism* / Q000382:microbiology; D014621:Vagina / Q000382:microbiology / Q000473:pathology
chemical_list: D011460:Prostaglandins F; C008228:15-keto-13,14-dihydroprostaglandin F2alpha; D004970:Estrone; D013739:Testosterone; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol; D011388:Prolactin; D007986:Luteinizing Hormone; D015237:Dinoprost; D006854:Hydrocortisone