title: Cyclooxygenase-2 expression in Schwann cells and macrophages in the sciatic nerve after single spinal nerve injury in rats.
abstract: Recent evidence suggested that cyclooxygenase-2 (COX-2) expression in the peripheral nerve early after nerve injury might be involved in the development of neuropathic pain. Although previous investigators have demonstrated that COX-2 is expressed in peripheral nerve at a late phase (2-4 weeks) after nerve injury, COX-2 up-regulation at an early phase after nerve injury has not been determined. Using immunohistochemistry, we observed biphasic increases of COX-2 expression after L5 single spinal nerve injury. The first increment of COX-2 positive cells was noted 1 day after nerve injury and these cells co-expressed the Schwann cell marker S-100. A second increment was noted after 7-14 days and these cells co-expressed the macrophage marker ED-1. These results suggested that the cellular sources of COX-2 expression might be different between the early and late phases after nerve injury.
authors.affiliation: Department of Anesthesiology, Nara Medical University, Kashihara, Nara 634-8521, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Takahashi
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kawaguchi
authors.forename: Keiji
authors.initials: K
authors.lastname: Shimada
authors.forename: Noboru
authors.initials: N
authors.lastname: Konishi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Furuya
authors.forename: Toshikatsu
authors.initials: T
authors.lastname: Nakashima
mesh_terms: D000818:Animals; D002452:Cell Count; D051546:Cyclooxygenase 2; D053331:Ectodysplasins; D007839:Functional Laterality / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods; D007527:Isoenzymes / Q000378:metabolism*; D008264:Macrophages / Q000201:enzymology*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D009418:S100 Proteins / Q000378:metabolism; D012583:Schwann Cells / Q000201:enzymology*; D012584:Sciatic Nerve / Q000166:cytology*; D013119:Spinal Cord Injuries / Q000201:enzymology* / Q000378:metabolism; D013997:Time Factors
chemical_list: D053331:Ectodysplasins; D007527:Isoenzymes; D008565:Membrane Proteins; D009418:S100 Proteins; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases