Isolation of high quality RNA from Streptomyces.
Several molecular techniques require high quality RNA, completely free of DNA. Standard methods to isolate total RNA from Streptomyces spp. are based on the application of a 'Modified Kirby Mix' [Practical Streptomyces Genetics. The John Innes Foundation, Norwich, pp. 613]. Here we present an alternative procedure using Triton X-100 and EDTA for the isolation of total RNA from Streptomyces.
Van Dessel
W
W
Laboratory of Bacteriology, Rega Institute, K.U.Leuven, 10 Minderbroedersstraat, B-3000 Leuven, Belgium.
Van Mellaert
L
L
Geukens
N
N
Lammertyn
E
E
Anné
J
J
D004492:Edetic Acid / Q000737:chemistry; D006146:Guanidines / Q000737:chemistry; D017830:Octoxynol / Q000737:chemistry; D010636:Phenols / Q000737:chemistry; D012329:RNA, Bacterial / Q000302:isolation & purification*; D013302:Streptomyces / Q000737:chemistry / Q000235:genetics*
D006146:Guanidines; D010636:Phenols; D012329:RNA, Bacterial; C411644:trizol; D017830:Octoxynol; D004492:Edetic Acid