Chemical structure of aeromonas gum--extracellular polysaccharide from Aeromonas nichidenii 5797.
Aeromonas (A) gum, an extracellular heteropolysaccharide produced by the bacterium Aeromonas nichidenii strain 5797, was studied by 1H and 13C NMR spectroscopy including 2D COSY, TOCSY, 1H, 13C HMQC, HMBC and ROESY experiments after O-deacetylation and Smith degradation. These investigations revealed the presence of an O-acetylated pentasaccharide repeating unit composed of mannose, glucose, xylose and glucuronic acid, and it has the following structure: [Image: see text]
Xu
Xiaojuan
X
Department of Chemistry, Wuhan University, 430072, China.
Ruan
Dong
D
Jin
Yong
Y
Shashkov
Alexander S
AS
Senchenkova
Sof'ya N
SN
Kilcoyne
Michelle
M
Savage
Angela V
AV
Zhang
Lina
L
D000333:Aeromonas / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D002849:Chromatography, Gas; D005947:Glucose / Q000032:analysis / Q000737:chemistry; D020723:Glucuronic Acid / Q000032:analysis / Q000737:chemistry; D008358:Mannose / Q000032:analysis / Q000737:chemistry; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D012015:Reference Standards; D013696:Temperature; D014994:Xylose / Q000032:analysis / Q000737:chemistry
D002247:Carbon Isotopes; D011134:Polysaccharides; D020723:Glucuronic Acid; D014994:Xylose; D005947:Glucose; D008358:Mannose