Effects of HMGB-1 overexpression on cell-cycle progression in MCF-7 cells.
High mobility group-1 (HMGB-1) enhances the DNA interactions and possesses a transcriptional activation potential for several families of sequence-specific transcriptional activators. In order to examine the effect of HMGB-1 on the cell cycle progression in MCF-7 cells, the HMGB-1 expression vector was transfected into synchronized MCF-7 cells, and the effect of HMGB-1 overexpression on the cell cycle was examined. The HMGB-1 protein level in the transfected cells increased 4.87-fold compared to the non-transfected cells. There were few changes in the cell cycle phase distribution after HMGB-1 overexpression in the MCF-7 cells. Following the estrogen treatment, the cell cycle progressed in both the HMGB-1 overexpressed MCF-7 and the mock-treated cells. However, a larger proportion of HMGB-1 overexpressing MCF-7 cells progressed to the either S or G2 phase than the mock-treated cells. The mRNA levels of the cell cycle regulators changed after being treated with estrogen in both the HMGB-1 overexpressing MCF-7 and the mock-treated cells, but the changes in the expression level of the cell cycle regulator genes were more prominent in the HMGB-1 overexpressing MCF-7 cells than in the mock-treated cells. In conclusion, HMGB-1 overexpression itself does not alter the MCF-7 cell cycle progression, but the addition of estrogen to the HMGB-1 overexpressing MCF-7 cells appears to accelerate the cell cycle progression.
Yoon
Sarah
S
Center for Clinical Medicine & Samsung Biomedical Research Institute, University School of Medicine, Seoul, Korea.
Lee
Jin Young
JY
Yoon
Byung-Koo
BK
Bae
Duk Soo
DS
Choi
Doo Seok
DS
D015153:Blotting, Western; D002453:Cell Cycle; D045744:Cell Line, Tumor; D003720:Densitometry; D004967:Estrogens / Q000378:metabolism; D016195:G2 Phase; D005822:Genetic Vectors; D024243:HMGB1 Protein / Q000096:biosynthesis*; D006801:Humans; D007700:Kinetics; D009841:Oligonucleotides / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016196:S Phase; D013997:Time Factors; D015533:Transcriptional Activation; D014162:Transfection
D004967:Estrogens; D024243:HMGB1 Protein; D009841:Oligonucleotides; D012333:RNA, Messenger