A wide-range integrative yeast expression vector system based on Arxula adeninivorans-derived elements.
An Arxula adeninivorans integration vector was applied to a range of alternative yeast species including Saccharomyces cerevisiae, Debaryomyces hansenii, Debaryomyces polymorphus, Hansenula polymorpha and Pichia pastoris. The vector harbours a conserved A. adeninivorans-derived 25S rDNA sequence for targeting, the A. adeninivorans-derived TEF1 promoter for expression control of the reporter sequence, and the Escherichia coli-derived hph gene conferring resistance against hygromycin B for selection of recombinants. Heterologous gene expression was assessed using a green fluorescent protein (GFP) reporter gene. The plasmid was found to be integrated into the genome of the various hosts tested; recombinant strains of all species exhibited heterologous gene expressions of a similar high level.
Terentiev
Yaroslav
Y
Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstrasse 3, 06466 Gatersleben, Germany.
Pico
Almudena Huarto
AH
Böer
Erik
E
Wartmann
Thomas
T
Klabunde
Jens
J
Breuer
Uta
U
Babel
Wolfgang
W
Suckow
Manfred
M
Gellissen
Gerd
G
Kunze
Gotthard
G
D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D004718:Saccharomycetales / Q000235:genetics*; D014170:Transformation, Genetic; D015003:Yeasts / Q000235:genetics* / Q000378:metabolism
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins