title: Fluorescence optical diffusion tomography using multiple-frequency data.
abstract: A method is presented for fluorescence optical diffusion tomography in turbid media using multiple-frequency data. The method uses a frequency-domain diffusion equation model to reconstruct the fluorescent yield and lifetime by means of a Bayesian framework and an efficient, nonlinear optimizer. The method is demonstrated by using simulations and laboratory experiments to show that reconstruction quality can be improved in certain problems through the use of more than one frequency. A broadly applicable mutual information performance metric is also presented and used to investigate the advantages of using multiple modulation frequencies compared with using only one.
authors.affiliation: School of Electrical and Computer Engineering, Purdue University, West Lafayette, Indiana 47907-2035, USA.
authors.forename: Adam B
authors.initials: AB
authors.lastname: Milstein
authors.forename: Jonathan J
authors.initials: JJ
authors.lastname: Stott
authors.forename: Seungseok
authors.initials: S
authors.lastname: Oh
authors.forename: David A
authors.initials: DA
authors.lastname: Boas
authors.forename: R P
authors.initials: RP
authors.lastname: Millane
authors.forename: Charles A
authors.initials: CA
authors.lastname: Bouman
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Webb
mesh_terms: D000465:Algorithms; D003198:Computer Simulation; D003238:Connective Tissue / Q000378:metabolism* / Q000648:ultrastructure*; D004058:Diffusion; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D008954:Models, Biological; D019047:Phantoms, Imaging; D013050:Spectrometry, Fluorescence / Q000379:methods*; D041622:Tomography, Optical / Q000379:methods*