Inhibition of the gamma interferon response by a Sendai virus C protein mutant with no STAT1-binding ability.
Sendai virus C protein interacts with the signal transducer and activator of transcription (STAT) 1. This interaction is believed to be essential for the Sendai virus inhibition of the interferon (IFN) response. We here analyzed C(F170S) (a C protein mutant with the F170S mutation) with no STAT1-binding ability. C(F170S) lacked the ability to inhibit the IFN-alpha response, but retained the ability to inhibit the IFN-gamma response. IFN-gamma stimulation caused STAT1 phosphorylation, formation of the gamma-activated factor capable of binding to a gamma-activated sequence DNA probe, and STAT1 nuclear translocation, even in the presence of C(F170S). These results suggest that C protein has the STAT1-binding-independent anti-IFN-gamma mechanism, which targets processes after the STAT1 nuclear translocation event.
Gotoh
Bin
B
Microbiology Section, Department of Pathological Sciences, Faculty of Medical Sciences, University of Fukui, Shimoaizuki 23-3, Matsuoka-cho, Yoshida-gun, Fukui 910-1193, Japan. bin@fmsra.fukui-med.ac.jp
Takeuchi
Kenji
K
Komatsu
Takayuki
T
D019943:Amino Acid Substitution; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D007371:Interferon-gamma / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008156:Luciferases / Q000378:metabolism; D010748:Phosphopeptides / Q000378:metabolism; D010766:Phosphorylation; D011485:Protein Binding; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D050794:STAT1 Transcription Factor; D029082:Sendai virus / Q000737:chemistry; D015534:Trans-Activators / Q000378:metabolism*; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*
D004268:DNA-Binding Proteins; D010748:Phosphopeptides; D011993:Recombinant Fusion Proteins; D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; D015534:Trans-Activators; D014764:Viral Proteins; C051221:nonstructural C protein, Sendai virus; D007371:Interferon-gamma; D008156:Luciferases