NMR solution structure and position of transportan in neutral phospholipid bicelles.
Transportan is a chimeric cell-penetrating peptide constructed from the peptides galanin and mastoparan, which has the ability to internalize living cells carrying a hydrophilic load. In this study, we have determined the NMR solution structure and investigated the position of transportan in neutral bicelles. The structure revealed a well-defined alpha-helix in the C-terminal mastoparan part of the peptide and a weaker tendency to form an alpha-helix in the N-terminal domain. The position of the peptide in relation to the membrane, as studied by adding paramagnetic probes, shows that the peptide lies parallel to, and in the head-group region of the membrane surface. This result is supported by amide proton secondary chemical shifts.
Bárány-Wallje
Elsa
E
Department of Biochemistry and Biophysics, The Arrhenius Laboratories, Stockholm University, S-10691 Stockholm, Sweden.
Andersson
August
A
Gräslund
Astrid
A
Mäler
Lena
L
D000577:Amides / Q000737:chemistry; D000595:Amino Acid Sequence; D002942:Circular Dichroism; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D019004:Galanin; D005994:Glycerophosphates / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D008823:Micelles; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010742:Phospholipid Ethers / Q000737:chemistry*; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry*; D013113:Spin Labels; D014862:Wasp Venoms
C052298:1,2-dihexadecyl-sn-glycero-3-phosphocholine; D000577:Amides; D004337:Drug Carriers; D005994:Glycerophosphates; D008051:Lipid Bilayers; D008823:Micelles; D010742:Phospholipid Ethers; D011993:Recombinant Fusion Proteins; D013113:Spin Labels; D014862:Wasp Venoms; C110068:transportan; D010767:Phosphorylcholine; C028810:dodecylphosphocholine; D019004:Galanin; D004134:Dimyristoylphosphatidylcholine