[Development of human myeloid leukemia-like phenotype in NUP98-PMX1 transgenic mice].
OBJECTIVE
In order to investigate the leukemogenic potential of NUP98-PMX1 fusion gene in vivo.


METHODS
NUP98-PMX1 transgenic mice were generated, in which the fusion gene was driven by hCG promoter and expressed in myeloid cells at early stage of differentiation. Molecular cloning technology was used to construct NUP98-PMX1 transgenic plasmid. The genotype and phenotype of the NUP98-PMX1 transgenic mice were analyzed by PCR, RT-PCR, peripheral blood count (PBC), bone marrow (BM) cells morphology and pathological examination.


RESULTS
NIH3T3 cells transfected with NUP98-PMX1 fusion gene grew faster, formed colonies in soft agar, and developed tumors in 10 inoculated nude mice. Among 8 disordered NUP98-PMX1 transgenic mice, 4 developed myeloid leukemia-like phenotype, including 3 resembling human chronic myeloid leukemia.


CONCLUSIONS
NUP98-PMX1 has oncogenic activity and plays a crucial role in leukemogenesis.
Wang
Yang
Y
Lab of Medical & Molecular Genetics, Department of Medical Genetics, Shanghai Second Medical University, Shanghai 200025, China.
Gu
Ming-min
MM
Tan
Yi
Y
Lu
Shun-yuan
SY
Wang
Long
L
Kong
Hui
H
Sun
Yue-ping
YP
Lu
Zhen-yu
ZY
Chen
Sai-juan
SJ
Wang
Zhen-yi
ZY
Wang
Zhu-gang
ZG
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D015973:Gene Expression Regulation, Leukemic; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008819:Mice, Nude; D008822:Mice, Transgenic; D041681:NIH 3T3 Cells; D028861:Nuclear Pore Complex Proteins / Q000235:genetics* / Q000378:metabolism; D015514:Oncogene Proteins, Fusion / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
C120274:NUP98-PMX1 fusion protein, human; D028861:Nuclear Pore Complex Proteins; D015514:Oncogene Proteins, Fusion; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins