Effect of social isolation on stress-related behavioural and neuroendocrine state in the rat.
The present study investigated the effects of post-weaning social isolation (SI) on behavioural and neuroendocrine reactivity to stress of male and female rats. Innate aspects of fear and anxiety were assessed in the open field and elevated plus maze tests. Spontaneous startle reflex and conditioned fear response were further investigated. The neuroendocrine response of isolates was examined by measuring basal and stress release of ACTH and corticosterone and by evaluating the mRNA expression of mineralocorticoid (MR) and glucocorticoid (GR) receptors using in situ hybridization. Locomotor activity in the open field was not modified by chronic SI. In males, but not females, SI produced an anxiogenic profile in the elevated plus maze. Male isolates showed a trend towards increased startle reflex amplitude relative to socially-reared controls. Moreover, SI in males produced alterations of the HPA axis functioning as reflected by higher basal levels of ACTH, and enhanced release of ACTH and corticosterone following stress. In contrast, startle response or HPA axis functioning were not altered in female isolates. Social isolates from both genders showed reduced contextual fear-conditioning. Finally, the mRNA expression of MR and GR was not modified by SI. The results of the present study suggest that chronic SI increases emotional reactivity to stress and produces a hyperfunction of the HPA axis in adult rats, particularly in males.
Weiss
Isabelle C
IC
Behavioural Neurobiology Laboratory, Swiss Federal Institute of Technology Zurich, Schorenstrasse 16, 8603 Schwerzenbach, Switzerland.
Pryce
Christopher R
CR
Jongen-Rêlo
Ana L
AL
Nanz-Bahr
Nina I
NI
Feldon
Joram
J
D000324:Adrenocorticotropic Hormone / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001522:Behavior, Animal / Q000502:physiology*; D001921:Brain / Q000378:metabolism; D003213:Conditioning, Psychological; D003345:Corticosterone / Q000097:blood; D005239:Fear; D005260:Female; D005938:Glucocorticoids / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D007668:Kidney / Q000378:metabolism; D008297:Male; D018782:Maze Learning / Q000502:physiology; D009043:Motor Activity / Q000502:physiology; D009490:Neurosecretory Systems / Q000502:physiology*; D011863:Radioimmunoassay / Q000379:methods; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D018161:Receptors, Mineralocorticoid / Q000378:metabolism; D012022:Reflex, Acoustic / Q000502:physiology; D012737:Sex Factors; D012934:Social Isolation / Q000523:psychology*; D013312:Stress, Physiological / Q000378:metabolism / Q000503:physiopathology*; D014886:Weaning
D005938:Glucocorticoids; D018161:Receptors, Mineralocorticoid; D000324:Adrenocorticotropic Hormone; D003345:Corticosterone