Tissue alterations in the Guinea pig lateral prostate following antiandrogen flutamide therapy.
The flutamide antiandrogenic effects on the Guinea pig male prostate morphology in puberal, post-puberal and adult ages were evaluated in the present study. Daily-treated group animals received flutamide subcutaneous injection at a dose of 10 mg/Kg body weight for 10 days. The control group animals received a pharmacological vehicle under the same conditions. The lateral prostate was removed, fixed and processed for light and transmission electron microscopy. The results revealed an increase of the acinus diameter in the treated puberal animals and straitness in the stromal compartment around the acini. The epithelial cells exhibited cubic phenotype. In the post-puberal and adult animals, a decrease of the acinus diameter was observed, as well as an increase of the smooth muscle layer and presence of the folds at epithelium. The ultrastructural evaluation of the secretory cells in the treated group demonstrated endomembrane enlargement, mainly in the rough endoplasmic reticulum and Golgi apparatus. In addition, a decrease of the microvilli and alterations in the distribution patterns and density of the stromal fibrillar components were observed. In conclusion, the flutamide treatment exerts tissue effects on the lateral prostate, promoting stroma/epithelium alterations.
Cordeiro
Renato Simões
RS
Department of Cell Biology, Institute of Biology, UNICAMP, CP 6109, 13084-971, Campinas, SP, Brazil.
Scarano
Wellerson Rodrigo
WR
Góes
Rejane Maira
RM
Taboga
Sebastião Roberto
SR
D000367:Age Factors; D000726:Androgen Antagonists / Q000494:pharmacology*; D000818:Animals; D048429:Cell Size / Q000187:drug effects / Q000502:physiology; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000648:ultrastructure; D004847:Epithelial Cells / Q000187:drug effects* / Q000648:ultrastructure; D005485:Flutamide / Q000494:pharmacology*; D006056:Golgi Apparatus / Q000187:drug effects / Q000648:ultrastructure; D006168:Guinea Pigs; D008297:Male; D008854:Microscopy, Electron; D008871:Microvilli / Q000187:drug effects / Q000648:ultrastructure; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000648:ultrastructure; D011467:Prostate / Q000187:drug effects* / Q000648:ultrastructure; D012741:Sexual Maturation; D017154:Stromal Cells / Q000187:drug effects / Q000648:ultrastructure
D000726:Androgen Antagonists; D005485:Flutamide