Role of reporter gene imaging in molecular and cellular biology.
Molecular imaging, including reporter gene methods, provides a unique opportunity to study biology in a living subject, thereby allowing physiological events to be monitored in an intact microenvironment. This review takes a molecular and cell biology perspective on recent studies which utilize reporter gene imaging as a tool to non-invasively monitor specific molecular biology pathways in vivo. Studies in rodent models demonstrate the feasibility of reporter gene imaging to visualize and measure key cellular pathways, such as transcription, translation and protein-protein interactions. The review indicates that molecular imaging is likely to be useful in the translation of molecular biology to medicine and biotechnological applications.
Mayer-Kuckuk
Philipp
P
Department of Pharmacology, The Cancer Institute of New Jersey, UMDNJ, New Brunswick, NJ 08903, USA. mayerkup@mskcc.org
Menon
Lata G
LG
Blasberg
Ronald G
RG
Bertino
Joseph R
JR
Banerjee
Debabrata
D
D000818:Animals; D015870:Gene Expression; D017930:Genes, Reporter; D008163:Luminescent Measurements / Q000379:methods; D051379:Mice; D008967:Molecular Biology; D055095:Optics and Photonics; D049268:Positron-Emission Tomography / Q000379:methods; D011506:Proteins / Q000378:metabolism; D015398:Signal Transduction; D015533:Transcriptional Activation
D011506:Proteins