Flow-injection determination of isoniazid using sodium dichloroisocyanurate- and trichloroisocyanuric acid-luminol chemiluminescence systems.
A chemiluminescent (CL) method for the determination of isoniazid is described. The method is based on the CL generated during the oxidation of luminol by sodium dichloroisocyanurate (SDCC) and trichloroisocyanuric acid (TCCA) in alkaline medium. It was found that isoniazid greatly enhances this CL intensity when present in the luminol solution. Based on this observation, a new flow-injection CL method for the determination of isoniazid has been proposed in this paper. The detection limits were 2 and 3 ng ml(-1) isoniazid for the SDCC-luminol and TCCA-luminol CL systems, respectively. The relative CL intensity was linear with the isoniazid concentration in the range of 4-100 and 100-200 ng ml(-1) for the SDCC-luminol CL system, and 6-200 and 200-1000 ng ml(-1) for the TCCA-luminol CL system. The results obtained for the assay of pharmaceutical preparations compared well with those obtained by the official methods and demonstrated good accuracy and precision.
Safavi
A
A
Department of Chemistry, Faculty of Sciences, Shiraz University, Shiraz 71454, Iran. savafi@chem.susc.ac.ir
Karimi
M A
MA
Hormozi Nezhad
M R
MR
D002623:Chemistry Techniques, Analytical / Q000379:methods; D017022:Flow Injection Analysis / Q000295:instrumentation; D005544:Forecasting; D007492:Iran; D007538:Isoniazid / Q000032:analysis*; D008163:Luminescent Measurements; D008165:Luminol / Q000031:analogs & derivatives* / Q000737:chemistry*; D016877:Oxidants / Q000737:chemistry; D012972:Sodium Hydroxide / Q000737:chemistry; D014227:Triazines / Q000737:chemistry*
D016877:Oxidants; D014227:Triazines; C500992:trichloroisocyanuric acid-luminol; D012972:Sodium Hydroxide; D008165:Luminol; C011765:troclosene; D007538:Isoniazid