title: Preparation and structure elucidation of alginate oligosaccharides degraded by alginate lyase from Vibro sp. 510.
abstract: Alginate that was purified from the fermentation solution of marine bacteria Vibro sp. 510 under specific reaction conditions was hydrolyzed by alginate lyase. Seven oligosaccharides, including di-, tri- and tetrasaccharides, were isolated through low-pressure, gel-permeation chromatography (LP-GPC) and semipreparative strong-anion exchange (SAX) fast-protein liquid chromatography (FPLC). The oligosaccharide structures were elucidated based on ESIMS and 2D NMR spectral analysis. The hydrolytic specificity of this alginate lyase to alginate is discussed.
authors.affiliation: Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, China.
authors.forename: Zhenqing
authors.initials: Z
authors.lastname: Zhang
authors.forename: Guangli
authors.initials: G
authors.lastname: Yu
authors.forename: Huashi
authors.initials: H
authors.lastname: Guan
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.forename: Yuguo
authors.initials: Y
authors.lastname: Du
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001419:Bacteria / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D020723:Glucuronic Acid / Q000737:chemistry*; D006603:Hexuronic Acids / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D011133:Polysaccharide-Lyases / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D009844:Oligosaccharides; D020723:Glucuronic Acid; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase