Membrane-mediated capillary electrophoresis: interaction of cationic peptides with bicelles.
Electrokinetic capillary chromatography is applied to determine the membrane affinity of peptides using both 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) micelles and DHPC/1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) bicelles under controlled conditions. The effect of temperature and the bicelle q value in surface association with cationic peptides is studied. The cationic peptides selected have a well-defined membrane structure (indolicidin), induced secondary structure (melittin, magainin 2), or do not possess classical secondary structure (atrial natriuretic peptide (ANP) 1-28, 4-28, 5-27). Electrokinetic capillary chromatography facilitated by DMPC and DHPC additives provides a rapid means of estimating lipophilicity and screening for peptides that have membrane affinity.
Mills
John O
JO
Department of Chemistry, West Virginia University, Morgantown, WV, USA.
Holland
Lisa A
LA
D023181:Antimicrobial Cationic Peptides / Q000737:chemistry; D002784:Cholesterol / Q000737:chemistry; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D054806:Magainins; D008563:Membrane Lipids / Q000737:chemistry*; D008566:Membranes / Q000737:chemistry*; D008823:Micelles; D010742:Phospholipid Ethers / Q000737:chemistry*; D013696:Temperature; D029867:Xenopus Proteins
C052298:1,2-dihexadecyl-sn-glycero-3-phosphocholine; D023181:Antimicrobial Cationic Peptides; D054806:Magainins; D008563:Membrane Lipids; D008823:Micelles; D010742:Phospholipid Ethers; D029867:Xenopus Proteins; C073143:indolicidin; C053301:magainin 2 peptide, Xenopus; D002784:Cholesterol; D004134:Dimyristoylphosphatidylcholine