[Comparison of genomes of new gigantic Pseudomonas aeruginosa phages from native populations from different regions].
To study the genome diversity of bacteriophages from geographically distant natural populations, new giant phi KZ-like Pseudomonas aeruginosa phages isolated in two different regions were compared with earlier known phages of three species (phi KZ, Lin68, EL). A broad spectrum of lytic activity was demonstrated for all phi KZ-like phages. Phages of the phi KZ species proved to be common in natural populations of various regions, while IL- and Lin68-related phages were extremely rare. Most phi KZ-related phages had unique DNA restriction patterns, but the differences between these were only minor, and the genomes did not contain nonhomologous fragments. The spectrum of capsid polypeptides proved to be conserved in each species, and was proposed as a character necessary and sufficient for express classification of phages with an accuracy of species. Phages isolated in different geographical regions showed no substantial difference. Some phages only slightly differing in DNA restriction pattern from phi KZ may be used to study the origin of phi KZ genes coding for orthologs of proteins of unrelated species (other phages, pathogenic bacteria, eukaryotes).
Krylov
V N
VN
State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, 113545 Russia. krylov@genetika.ru
Burkal'tseva
M V
MV
Sykilinda
N N
NN
Pleteneva
E A
EA
Shaburova
O V
OV
Kadykov
V A
VA
Miller
S
S
Biebl
M
M
D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D016679:Genome, Viral; D008854:Microscopy, Electron; D017105:Pseudomonas Phages / Q000235:genetics* / Q000648:ultrastructure; D011550:Pseudomonas aeruginosa / Q000821:virology*