Developments in peptide and amide synthesis.
The solid-phase methodology is key for an effective synthesis of peptides, from a milligram scale for research to a multi-kilo scale for drug production. Indeed, small peptides containing up to 20-30 amino acids are most readily synthesized by a solid-phase strategy. Larger peptides (up to 60 amino acids) should be synthesized by a convergent approach (i.e. synthesis of protected constituent peptides in solid-phase and combination of these units in solution). Larger peptides and proteins are prepared by chemical ligation, where unprotected segments have been prepared in solid-phase.
Albericio
Fernando
F
Department of Organic Chemistry, Martí i Franqués 1, University of Barcelona, 08028-Barcelona, Spain. albericio@pcb.ub.es
D000577:Amides / Q000138:chemical synthesis*; D000596:Amino Acids / Q000737:chemistry; D002625:Chemistry, Organic / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry; D008956:Models, Chemical; D010455:Peptides / Q000138:chemical synthesis*; D010456:Peptides, Cyclic / Q000138:chemical synthesis; D012116:Resins, Plant / Q000737:chemistry
D000577:Amides; D000596:Amino Acids; D003432:Cross-Linking Reagents; D010455:Peptides; D010456:Peptides, Cyclic; D012116:Resins, Plant