[Stimulating the expression of macrophage inflammatory protein-2gamma by lipopolysaccharide in-vitro].
OBJECTIVE
To study the expression level of macrophage inflammatory protein-2gamma(MIP-2gamma)stimulated by lipopolysaccharide(LPS)in-vitro.


METHODS
Murine monocyte macrophage like cell line RAW264.7 and BALB/c mice primary kidney cell culture were stimulated with LPS. MIP-2gamma mRNA expression level in these cells was detected by real time fluorescence quantitative RT-PCR.


RESULTS
MIP-2gamma mRNA expression level of RAW264.7 cell line increased quickly and reached the peak (7 times the normal level) within 2 hours after LPS stimulation and dropped to the base level within 16 hours, while that of cultured promary kidney cells of BALB/c mice elevated about 50 times compared with base level 12 hours following LPS stimulation.


CONCLUSIONS
LPS can significantly stimulate the expression of MIP-2gamma mRNA, suggesting that MIP-2gamma may play some role in inflammatory process.
Yang
Jia-hui
JH
Department of Laborotory Diagnosis, Changhai Hospital, Secondary Military Medical University, Shanghai 200433, China. yjhsmmu@yahoo.com.cn
Shen
Qian
Q
D000818:Animals; D054426:Chemokine CXCL2; D015870:Gene Expression / Q000187:drug effects; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D015846:Monokines / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis
D054426:Chemokine CXCL2; D008070:Lipopolysaccharides; D015846:Monokines; D012333:RNA, Messenger