title: Multiplex PCR assay for rapid detection and genotyping of Helicobacter pylori directly from biopsy specimens.
abstract: We developed and evaluated a simple, novel multiplex PCR assay for rapid detection of Helicobacter pylori infection and for the determination of vacA and cagA genotypes directly from gastric biopsy specimens. This assay did not require culturing of strains or extraction of DNA from biopsy samples. This multiplex PCR assay would be of particularly great value for laboratories in developing countries.
authors.affiliation: National Institute of Cholera and Enteric Diseases, Calcutta 700010, India.
authors.forename: Santanu
authors.initials: S
authors.lastname: Chattopadhyay
authors.forename: Rajashree
authors.initials: R
authors.lastname: Patra
authors.forename: T
authors.initials: T
authors.lastname: Ramamurthy
authors.forename: Abhijit
authors.initials: A
authors.lastname: Chowdhury
authors.forename: Amal
authors.initials: A
authors.lastname: Santra
authors.forename: G K
authors.initials: GK
authors.lastname: Dhali
authors.forename: S K
authors.initials: SK
authors.lastname: Bhattacharya
authors.forename: Douglas E
authors.initials: DE
authors.lastname: Berg
authors.forename: G Balakrish
authors.initials: GB
authors.lastname: Nair
authors.forename: Asish K
authors.initials: AK
authors.lastname: Mukhopadhyay
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001706:Biopsy; D005838:Genotype; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori