Antitumor activity of levan polysaccharides from selected microorganisms.
Levans were isolated from the cultures of Gluconoacetobacter xylinus (G-levan; Mw = 40,000), Microbacterium laevaniformans (M; Mw = 710,000), Rahnella aquatilis (R; Mw = 380,000), and Zymomonas mobilis (Z; Mw = 570,000). The levans were composed mainly of fructose residues when analyzed by TLC and HPLC, and their main backbones were beta-(2,6)-linkages with beta-(2,1)-branches by GC-MS and NMR. In the in vitro antitumor activity test of the levans against eight different tumor cell lines, relatively stronger activity was observed from the SNU-1 and HepG2. The M- (52.54-62.05%) and R-levan (52.15-58.58%) showed the significantly high activity against SNU-1, while M-levan showed the highest (49.93-61.82%) activity against HepG2. During the in vivo analysis of inhibitory activity of the levans against Sarcoma-180 growth, M-, R- and Z-levans showed strong antitumor activity (average 66%) but G-levan (42%) had significantly lower activity.
Yoo
Sang-Ho
SH
Department of Food Science and Technology, Sejong University, 98 Gunja-dong, Gwangjin-gu, Seoul 143-747, South Korea.
Yoon
Eun Ju
EJ
Cha
Jaeho
J
Lee
Hyeon Gyu
HG
D000192:Actinomycetales / Q000737:chemistry; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002236:Carbohydrate Conformation; D002241:Carbohydrates / Q000032:analysis; D004354:Drug Screening Assays, Antitumor; D005630:Fructans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D020576:Gluconobacter / Q000737:chemistry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008813:Mice, Inbred ICR; D015394:Molecular Structure; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D020638:Rahnella / Q000737:chemistry; D012513:Sarcoma, Experimental / Q000188:drug therapy; D014407:Tumor Cells, Cultured; D017355:Zymomonas / Q000737:chemistry
D000970:Antineoplastic Agents; D002241:Carbohydrates; D005630:Fructans; D011135:Polysaccharides, Bacterial; C072599:levan