Post-ovulatory ageing and egg quality: a proteomic analysis of rainbow trout coelomic fluid.
BACKGROUND
In fish, oocyte post-ovulatory ageing is associated with egg quality decrease. During this period, eggs are held in the body cavity where they bath in a semi-viscous liquid known as coelomic fluid (CF). CF components are suspected to play a role in maintaining oocyte fertility and developmental competence (egg quality). However, CF proteic composition remains poorly studied. Thus rainbow trout CF proteome was studied during the egg quality decrease associated with oocyte post-ovulatory ageing.


METHODS
High resolution two-dimensional gel electrophoresis was used to analyze the proteome of rainbow trout (Oncorhynchus mykiss) CF in relationship with the egg quality decrease associated with oocyte post-ovulatory ageing. A first experiment was performed using CF pools originating from 17 females sampled at ovulation as well as 7, 14 and 21 days later. These observations were verified using a second set of CF pools originating from 22 females sampled 5 and 16 days following ovulation.


RESULTS
Approximately 200 protein spots of 10-105 kDa molecular mass and 3-10 pI were detected in CF samples. Several protein spots, while undetected at the time of ovulation, exhibited a progressive and strong accumulation in CF during post-ovulatory ageing. After silver-staining and Matrix-Assisted Laser Desorption Time Of Flight (MALDI-TOF) mass spectrometer analysis, some of these protein spots were identified as lipovitellin II fragments.


CONCLUSIONS
These observations suggest that egg protein fragments accumulate in the CF during the post-ovulatory period and could therefore be used to detect egg quality defects associated with oocyte post-ovulatory ageing.
Rime
Hélène
H
Institut National de la Recherche Agronomique, SCRIBE, Campus de Beaulieu, 35042, Rennes Cedex, France. rime@beaulieu.rennes.inra.fr
Guitton
Nathalie
N
Pineau
Charles
C
Bonnet
Emilie
E
Bobe
Julien
J
Jalabert
Bernard
B
D000375:Aging / Q000502:physiology*; D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005260:Female; D029941:Fish Proteins / Q000378:metabolism; D008297:Male; D017686:Oncorhynchus mykiss / Q000235:genetics*; D009865:Oocytes / Q000502:physiology*; D010060:Ovulation / Q000502:physiology*; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
D029941:Fish Proteins; D020543:Proteome