title: [A cytogenetic and molecular genetic study on microdeletion of AZF region on Y chromosome].
abstract: OBJECTIVE
  To study the morphology of Y chromosome and microdeletion of the correlated specific azoospermia factor(AZF) region on Y chromosome in cases of azoospermia and to identify the genetic diagnosis made for male infertility patients.
  
  
  METHODS
  Peripheral blood samples were taken from two patients with azoospermia, and then were examined by use of G banding, C banding cytogenetic analysis and multiplex polymerase chain reaction (PCR) microdeletion analysis.
  
  
  RESULTS
  The karyotypes of the two cases were 45, X, -Y, -22, +der(Y)t(Y;22)(q11.2;q11.2) and 46, XY, del(Y)(q11.2) respectively. In 12 sequence-tagged sites(STS) of AZFa, AZFb, AZFd, AZFc, only one was detected in the first case and two were detected in the other case.
  
  
  CONCLUSIONS
  The cytogenetic analysis and the detection of AZF microdeletion on Y chromosome are essential to the final genetic diagnosis to be made for male infertility patients.
authors.affiliation: Laboratory of Genetics, Reproductive Medical Center, Shenzhen Hospital of Beijing University, Shenzhen, Gungdong, 518036 PR China. Xiaosu_xiao@yahoo.com.cn
authors.forename: Xiao-su
authors.initials: XS
authors.lastname: Xiao
authors.forename: Xiao-yi
authors.initials: XY
authors.lastname: Liu
authors.forename: Yong-qiang
authors.initials: YQ
authors.lastname: Wang
authors.forename: Yin-han
authors.initials: YH
authors.lastname: Zhang
authors.forename: Yuan-hui
authors.initials: YH
authors.lastname: Yang
authors.forename: Li-bing
authors.initials: LB
authors.lastname: Liao
authors.forename: Zhi-ming
authors.initials: ZM
authors.lastname: Cai
mesh_terms: D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics*; D056426:Genetic Loci; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D029589:Seminal Plasma Proteins / Q000235:genetics*
chemical_list: D029589:Seminal Plasma Proteins