[Construction of a DNA vaccine against human B cell lymphoma].
OBJECTIVE
To construct a DNA vaccine based on surface Ig V(H) gene of tumor cells in the human B-cell lymphoma biopsy tissue.


METHODS
The V(H) gene fragment was amplified by RT-PCR using Ig superfamily primers. Also, the murine monocyte chemotactic protein 3(MCP-3) cDNA was cloned. The fusion gene fragment of MCP-3 gene with V(H) gene was constructed by recombinant PCR and then cloned into the eukaryotic expression vector pcDNA3.1 to construct the DNA vaccine plasmid pcDNA3.1/MCP-V(H). The vaccine plasmid was transiently expressed in the eukaryotic cell line COS-7.


RESULTS
The DNA vaccine plasmid was successfully constructed and expressed in COS-7 cells in the form of fusion protein MCP-V(H).


CONCLUSIONS
The DNA vaccine plasmid pcDNA3.1/MCP-V(H) is constructed and expressed successfully, which plays the foundation for further experimental research in animal model.
Zhang
Yun-sheng
YS
Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
Zhong
Kai-li
KL
Yuan
Ye
Y
Zhang
Ming-wei
MW
Zhang
Yi
Y
Mao
Ning
N
D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D019496:Cancer Vaccines / Q000276:immunology*; D054410:Chemokine CCL7; D016207:Cytokines; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D016393:Lymphoma, B-Cell / Q000276:immunology*; D008969:Molecular Sequence Data; D018945:Monocyte Chemoattractant Proteins / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*
C515450:CCL7 protein, human; D019496:Cancer Vaccines; D054410:Chemokine CCL7; D016207:Cytokines; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D018945:Monocyte Chemoattractant Proteins; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA