title: [Preparation and characterization of rabbit antibody against human selenoprotein P].
abstract: OBJECTIVE
  To prepare rabbit antibody against human selenoprotein P (HSelP) by using HSelP expressed in the prokaryotic expression system and identify its specificity.
  
  
  METHODS
  HSelP gene fragment was expressed in E.Coli via IPTG induction and purified through DEAE and Ni-NTA columns sequentially. The purified HSelP as immunogen was used to immunize rabbit. The specificity of rabbit anti-HSelP antibody was analyzed by Western blot.
  
  
  RESULTS
  HSelP protein had been expressed and purified successfully. The polyclonal anti-HSelP antibody could specifically recognize natural HSelP from human serum.
  
  
  CONCLUSIONS
  Polyclonal anti-HSelP antibody with high specificity and purity has been prepared by using purified HSelP as immunogen, which lays the foundation for further research on the function and distribution of HSelP, as well as developing a simple method for detecting HSelP.
authors.affiliation: National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China. qingfang71@yahoo.com
authors.forename: Qing
authors.initials: Q
authors.lastname: Fang
authors.forename: Ming-cheng
authors.initials: MC
authors.lastname: Zhang
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Zha
authors.forename: Sheng-li
authors.initials: SL
authors.lastname: Bi
authors.forename: Zhi-wei
authors.initials: ZW
authors.lastname: Li
authors.forename: Jian-guo
authors.initials: JG
authors.lastname: Yang
authors.forename: Zhu-xiu
authors.initials: ZX
authors.lastname: Xiao
authors.forename: Chen
authors.initials: C
authors.lastname: Lin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D018076:DNA, Complementary / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D011817:Rabbits; D051149:Selenoprotein P; D051140:Selenoproteins
chemical_list: D000906:Antibodies; D018076:DNA, Complementary; D011506:Proteins; D051149:Selenoprotein P; D051140:Selenoproteins