[Detection of pentachlorophenol in biomaterial using HPLC].
OBJECTIVE
To develop a method of detecting pentachlorophenol in biomaterial.


METHODS
The samples were extracted by ether, and the characters of the high performance liquid chromatography (HPLC) method were studied.


RESULTS
A method for the detection of pentachlorophenol in fish tissue samples was set up with the conditions of chromatography as follows: the compounds are separated on Hypersil ODS column(4.6 mm i.d. x 150 mm, 5 microns) and eluted with methanol which contains 2 g/L NH4Ac (20:80) using 305 nm as the detecting wavelength, the recovery of method being 76%, the linear range 0.042 microgram/g-1.344 micrograms/g, and the correlation coefficient 0.9994, the limit of quantification 0.014 microgram/g. Finally, pentachlorophenol was successfully detected in the fish tissue samples.


CONCLUSIONS
The method has been proven useful in the forensic cases involved in the poisoning of pentachlorophenol.
Liao
Lin-chuan
LC
Department of Analytical Toxicology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Yan
You-yi
YY
Lin
Lan
L
Yang
Lin
L
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005399:Fishes; D005554:Forensic Medicine; D008099:Liver / Q000737:chemistry; D010416:Pentachlorophenol / Q000032:analysis*; D012680:Sensitivity and Specificity
D010416:Pentachlorophenol