Catalytic activation of multimeric RNase E and RNase G by 5'-monophosphorylated RNA.
RNase E is an endonuclease that plays a central role in RNA processing and degradation in Escherichia coli. Like its E. coli homolog RNase G, RNase E shows a marked preference for cleaving RNAs that bear a monophosphate, rather than a triphosphate or hydroxyl, at the 5' end. To investigate the mechanism by which 5'-terminal phosphorylation can influence distant cleavage events, we have developed fluorogenic RNA substrates that allow the activity of RNase E and RNase G to be quantified much more accurately and easily than before. Kinetic analysis of the cleavage of these substrates by RNase E and RNase G has revealed that 5' monophosphorylation accelerates the reaction not by improving substrate binding, but rather by enhancing the catalytic potency of these ribonucleases. Furthermore, the presence of a 5' monophosphate can increase the specificity of cleavage site selection within an RNA. Although monomeric forms of RNase E and RNase G can cut RNA, the ability of these enzymes to discriminate between RNA substrates on the basis of their 5' phosphorylation state requires the formation of protein multimers. Among the molecular mechanisms that could account for these properties are those in which 5'-end binding by one enzyme subunit induces a protein structural change that accelerates RNA cleavage by another subunit.
Jiang
Xunqing
X
Skirball Institute of Biomolecular Medicine and Department of Microbiology, New York University School of Medicine, New York, NY 10016, USA.
Belasco
Joel G
JG
D000595:Amino Acid Sequence; D002384:Catalysis; D003001:Cloning, Molecular; D004722:Endoribonucleases / Q000737:chemistry / Q000378:metabolism*; D004789:Enzyme Activation; D000939:Epitopes / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D029968:Escherichia coli Proteins; D016259:Genes, myc; D007700:Kinetics; D008969:Molecular Sequence Data; D010766:Phosphorylation; D010957:Plasmids; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity
D000939:Epitopes; D029968:Escherichia coli Proteins; D011506:Proteins; D011994:Recombinant Proteins; D012313:RNA; D004722:Endoribonucleases; C081905:rng protein, E coli; C022537:ribonuclease E