title: [Modified nested polymerase chain reaction in one tube for detection of Enterovirus].
abstract: A method of a modified nest-type polymerase chain reaction (MN-PCR), made in one tube, was elaborated that enhances the sensitivity and cuts the risk of cross-contamination in enteroviruses (EV) detection. The method, as described in detail above, was used to detect EV RNA in 76.9% and 31.25% of examined autopsy samples (13 liquor and 16 cardiac-tissue samples, respectively). It enabled the detection of EV RNA in 6.25% of samples that used to be negative, when tested by MN-PCR in 2 tubes. MN-PCR with one tube is a reliable, sensitive and specific diagnostic tool; it can be recommended for the routine diagnostics of enterovirus infection.
authors.forename: N V
authors.initials: NV
authors.lastname: Poklonskaia
authors.forename: T V
authors.initials: TV
authors.lastname: Amvros'eva
authors.forename: O V
authors.initials: OV
authors.lastname: D'iakonova
authors.forename: O B
authors.initials: OB
authors.lastname: Shcherbakova
authors.forename: R Ia
authors.initials: RIa
authors.lastname: Voĭlokova
mesh_terms: D004770:Enterovirus / Q000235:genetics* / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000175:diagnosis; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods