title: [Expression and biological activities of TRAIL fusion protein gene].
abstract: OBJECTIVE
  To obtain and express the full-length of TRAIL gene in prokaryocytes.
  
  
  METHODS
  The full-length of TRAIL gene was amplified from peripheral blood cells(PBCs)by RT-PCR and then cloned into the expression vector pGEX-2T. The TRAIL-GST was expressed in E.coli.
  
  
  RESULTS
  Sequence analysis indicated that the sequence of TRAIL gene was identical with that in the GenBank. The TRAIL-GST was expressed in E.coli. Expressed product could inhibit the proliferation of KB and Hela cells and finally lead to their apoptosis.
  
  
  CONCLUSIONS
  The TRAIL-GST with biological activity has been expressed in the prokaryotic system.
authors.affiliation: Institute of Beijing Infectious Diseases, Beijing 100039, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Zheng
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Du
authors.forename: Cui-li
authors.initials: CL
authors.lastname: Shu
authors.forename: Yun
authors.initials: Y
authors.lastname: Cheng
mesh_terms: D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins; D005434:Flow Cytometry; D005982:Glutathione Transferase / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D007624:KB Cells; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D053221:TNF-Related Apoptosis-Inducing Ligand; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000502:physiology
chemical_list: D051017:Apoptosis Regulatory Proteins; D008562:Membrane Glycoproteins; D011993:Recombinant Fusion Proteins; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; D014409:Tumor Necrosis Factor-alpha; D005982:Glutathione Transferase