Selenium distribution in a Se-enriched mushroom species of the genus Ganoderma.
Data reported here show that Ganoderma lucidum could biotransform inorganic selenite in the substrate into organic forms by intergrating Se into proteins (56-61%) and polysaccharides (11-18%) and other components. Furthermore, water- and alkaline-soluble protein components were mainly responsible for the storage of organic Se, and Se-Met accounts for only a minor (8.2-18.3%) amount of the selenocompounds present in proteins. The molecular mass of most proteins or protein subunits containing Se was no more than 16 kDa. A low concentration of Se (<100 microg/g) in the substrate facilitated the synthesis of total protein and amino acids in G. lucidum, but a high concentration of Se (>150 microg/g) played a reverse role. Additionally, Se concentration in the culture had no significant effect on the distribution of the amino acids and proteins.
Zhao
Lei
L
DAPR Laboratory, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, People's Republic of China.
Zhao
Guanghua
G
Zhao
Zhengdong
Z
Chen
Peng
P
Tong
Jiyun
J
Hu
Xiaosong
X
D000596:Amino Acids / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis / Q000737:chemistry; D032902:Ganoderma / Q000737:chemistry*; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry; D012643:Selenium / Q000032:analysis*; D051140:Selenoproteins; D014018:Tissue Distribution
D000596:Amino Acids; D005656:Fungal Proteins; D011506:Proteins; D051140:Selenoproteins; D012643:Selenium