title: Assessment of hormonal activity of UV filters in tadpoles of frog Xenopus laevis at environmental concentrations.
abstract: Residues of UV filters used as ultraviolet absorbers in sunscreens, cosmetics and in light protection have been found in surface water and fish. Recently some of them were shown bearing estrogenic activity, however, very little is known about possible adverse effects to aquatic life. Here we investigate whether two UV filters, 4-methylbenzylidene camphor (4-MBC) and 3-benzylidene camphor (3-BC), interfere with the thyroid and sex hormone system during frog metamorphosis. Xenopus laevis tadpoles were exposed to 1, 5 and 50 microg/L of 4-MBC and 3-BC, respectively, for 35 days (NF stage 52-66). The rate of metamorphosis was not affected, and no obvious differences in body and tail length compared to controls were observed. Neither 4-MBC, nor 3-BC led to effects on the sex ratio or gross gonad morphology of X. laevis at stage 66. Our results indicate that these UV filters do not negatively affect the thyroid system and sex ratio of frogs at environmental concentrations.
authors.affiliation: Springborn Smithers Laboratories (Europe) AG, Seestrasse 21, CH-9326 Horn, Switzerland.
authors.forename: Petra Y
authors.initials: PY
authors.lastname: Kunz
authors.forename: Héctor F
authors.initials: HF
authors.lastname: Galicia
authors.forename: Karl
authors.initials: K
authors.lastname: Fent
mesh_terms: D000818:Animals; D002164:Camphor / Q000031:analogs & derivatives*; D004305:Dose-Response Relationship, Drug; D006066:Gonads / Q000254:growth & development; D008675:Metamorphosis, Biological / Q000187:drug effects*; D012744:Sex Ratio; D013473:Sunscreening Agents / Q000633:toxicity*; D036401:Toxicity Tests, Chronic; D014982:Xenopus laevis / Q000254:growth & development* / Q000378:metabolism
chemical_list: D013473:Sunscreening Agents; D002164:Camphor; C038939:enzacamene