Naming progastrin-derived peptides.
The antral hormone gastrin continues to be in focus, because its hormonal and growth promoting effects are essential both for the function of the normal stomach and for the pathogenesis of major dyspeptic and neoplastic diseases. Deduction of the progastrin structure has improved the insight in the cellular synthesis of gastrin, but has also revealed that the biosynthetic machinery is complex, and, accordingly, that progastrin is processed to a multitude of more or less bioactive fragments. The naming of these fragments has, however, become inconsistent and confusing. Therefore, we propose a systematic nomenclature for progastrin-derived peptides of which there are three classes: (I) The gastrins with the evolutionary preserved tetrapeptide amide (Trp-Met-Asp-PheNH2) at the C-terminus, which ensures high-affinity binding to the gastrin (CCK-B) receptor. Among the gastrins, gastrin-34 and gastrin-17 constitute the primary forms. (II) Processing intermediates, which are early products of progastrin that contain the structure of the primary gastrins within their sequence, but still cannot bind the gastrin receptor due to insufficient processing at their C-terminus. (III) Flanking fragments from the N- and C-termini of progastrin that do not contain any primary gastrin in their sequence, but nevertheless may undergo posttranslational processing. Each fragment can be specified with suffixes corresponding to the derived sequence in progastrin.
Rehfeld
Jens F
JF
Department of Clinical Biochemistry (KB 3014), Rigshospitalet, University of Copenhagen, DK-2100, Denmark. rehfeld@rh.dk
Bundgaard
Jens R
JR
Goetze
Jens P
JP
Friis-Hansen
Lennart
L
Hilsted
Linda
L
Johnsen
Anders H
AH
D000595:Amino Acid Sequence; D000818:Animals; D005755:Gastrins / Q000737:chemistry* / Q000145:classification*; D006801:Humans; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000737:chemistry* / Q000145:classification*; D011499:Protein Processing, Post-Translational; D043887:Receptor, Cholecystokinin B / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D009626:Terminology as Topic
D005755:Gastrins; D011498:Protein Precursors; D043887:Receptor, Cholecystokinin B; C013706:big gastrin