FMRFamide tagging--how an ionotropic receptor can be used to measure peptide secretion.
In this chapter, we describe a technique, FMRFamide tagging, that in principle can be used to measure the release of any sequenced neuropeptide. The method relies upon the addition of an "electrophysiologically active" tag to the prohormone that encodes the neuropeptide of interest. Secretion of the electrophysiological tag (and thus the peptide of interest) is detected by activation of the ionotropic "tag receptor." Both the tagged prohormone and the tag receptor are expressed in the cell type under investigation. Since the tag and the neuropeptide of interest are on the same prohormone they are co-secreted and thus secretion of the tag reflects the co-secretion of the neuropeptide of interest. This method can be used to detect neuropeptide secretion on a millisecond timescale.
Whim
Matthew D
MD
Department of Biology, Penn State University, State College, PA 16802, USA. mdw13@psu.edu
Moss
Guy W J
GW
D000818:Animals; D002460:Cell Line; D019835:FMRFamide; D008564:Membrane Potentials; D009479:Neuropeptides / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D017470:Receptors, Glutamate / Q000502:physiology*; D018010:Receptors, Invertebrate Peptide / Q000502:physiology*; D011994:Recombinant Proteins; D014162:Transfection
C055209:FMRFamide receptor; D009479:Neuropeptides; D017470:Receptors, Glutamate; D018010:Receptors, Invertebrate Peptide; D011994:Recombinant Proteins; D019835:FMRFamide