title: Cardiac chimerism in recipients of peripheral-blood and bone marrow stem cells.
abstract: Multipotent progenitor cells have the ability to differentiate into most somatic cell types, including cardiac myocytes. We sought to investigate cardiac chimerism after peripheral-blood and bone marrow stem cell transplantation. Between 10 and 17 highly polymorphic short tandem repeat (STR) markers were assayed in DNA obtained from donors' peripheral blood, recipients' peripheral blood before transplantation, and the recipient's heart in every patient. Gender and non-gender STR donor alleles were identified in the recipient heart in three patients. Using a highly sensitive PCR assay to determine donor and recipient genotypes, we confirmed the existence of cardiac chimerism in recipients of peripheral-blood and bone marrow stem cells.
authors.affiliation: Cardiology Service, Hospital de la Santa Creu i Sant Pau, C/Sant Antoni Ma Claret 167, 08025 Barcelona, Spain. abayesgenis@hsp.santpau.es
authors.forename: Antoni
authors.initials: A
authors.lastname: Bayes-Genis
authors.forename: Eduardo
authors.initials: E
authors.lastname: Muñiz-Diaz
authors.forename: Lluis
authors.initials: L
authors.lastname: Catasus
authors.forename: Marina
authors.initials: M
authors.lastname: Arilla
authors.forename: Carmen
authors.initials: C
authors.lastname: Rodriguez
authors.forename: Jordi
authors.initials: J
authors.lastname: Sierra
authors.forename: Pedro J
authors.initials: PJ
authors.lastname: Madoz
authors.forename: Juan
authors.initials: J
authors.lastname: Cinca
mesh_terms: D000328:Adult; D000483:Alleles; D016026:Bone Marrow Transplantation; D005188:False Negative Reactions; D005260:Female; D005838:Genotype; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000628:therapy; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000628:therapy; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000166:cytology; D032383:Myocytes, Cardiac / Q000637:transplantation; D036102:Peripheral Blood Stem Cell Transplantation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020080:Tandem Repeat Sequences / Q000235:genetics; D014184:Transplantation, Homologous; D016896:Treatment Outcome