Immunohistochemical detection of growth hormone (GH) in canine hepatoid gland tumors.
The aim of this study was to detect immunohistochemically means growth hormone (GH) in 24 hepatoid gland adenomas and 5 hepatoid gland carcinomas and to compare the difference of immunoreactivity between types of tumors. The tumors were classified according to the WHO standards. Tissue sections which were prepared from formalin-fixed, paraffin wax-embedded tissues from 25 male and 4 female dogs were carried out immunostaining using polyclonal primary anti-hGH and EnVision method. Of 24 hepatoid gland adenomas (perianal gland adenomas) 23 (95.8%) were positive. All 5 hepatoid gland carcinomas (perianal gland carcinomas) were positive. No statistically significant differences in percentage of labelled cells between malignant and benign tumors were seen. The present demonstration of GH in hepatoid gland tumors adds new data on GH in extra-pituitary tissues and hormon-dependent tumors.
Petterino
Claudio
C
Department of Public Health, Comparative Pathology and Veterinary Hygiene, Faculty of Veterinary Medicine, University of Padua, Legnaro, Italy.
Martini
Marco
M
Castagnaro
Massimo
M
D000236:Adenoma / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D000694:Anal Gland Neoplasms / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D004283:Dog Diseases / Q000378:metabolism* / Q000473:pathology; D004285:Dogs; D013006:Growth Hormone / Q000378:metabolism*; D007150:Immunohistochemistry; D010481:Perianal Glands / Q000473:pathology; D010903:Pituitary Gland, Anterior / Q000473:pathology
D013006:Growth Hormone