[Cloning of a DnaJ homolog chaperon PBP and its subcellular localization].
OBJECTIVE
To isolate and identify a human DnaJ homolog chaperon, PBP, from a human skeleton cDNA library, and to analyze its expression and distribution in transfected mammalian cells.


METHODS
(32)p-dCTP labeled probe hybridization was used to screen the human skeleton cDNA library and sequence of the positive clones were analyzed. Then PBP gene was transfected into COS-7 cells using lipofectamin. PBP expressed in the cells were detected by Western-blot and indirect immunofluorescence staining.


RESULTS
A full-length(1.5 kb) cDNA of peripherin-binding protein (PBP) was identified, which is identical with that of mrj. Full length PBP was mainly localized to cytoplasms of COS-7 cells in interphase, and to nuclei in mitosis.


CONCLUSIONS
The results indicate that besides cooperating with DnaK (HSP70), PBP itself plays an important role as a member of DnaJ family. PBP may also be involved in the regulation of cell cycle.
Liu
Ying
Y
Center for Clinical Molecular Biology Research, Airforce General Hospital of Chinese PLA, Beijing 100036, China. yingr21hotmail.com
Zhu
Mei-cai
MC
Wang
Yin-jing
YJ
Zhan
Zhi
Z
Liu
Cheng-gang
CG
D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D019556:COS Cells; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D003001:Cloning, Molecular; D006224:Cricetinae; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D018832:Molecular Chaperones / Q000032:analysis / Q000235:genetics*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064531:Peripherins
D002352:Carrier Proteins; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D018832:Molecular Chaperones; D009419:Nerve Tissue Proteins; C578994:PRPH protein, human; D064531:Peripherins