title: Liquid-liquid extraction of xylitol dehydrogenase from Candida guilliermondii homogenate by reversed micelles.
abstract: The intracellular enzyme xylitol dehydrogenase (XD, EC 1.1.1.9) from Candida guilliermondii, grown in sugarcane bagasse hydrolysate, was separated by reversed micelles of BDBAC [N-benzyl-N-dodecyl-N-bis (2-hydroxyethyl) ammonium chloride] cationic surfactant. An experimental design was employed to evaluate the influence of the following factors on the enzyme separation: temperature, co-solvent concentration and surfactant concentration. The results showed that just the temperature did not show significant effect on XD recovery. A model was used to represent the activity recovery and fit the experimental data. Under optimized conditions, the recovery of total activity was about 121%, and the purity increased 2.3-fold.
authors.affiliation: Biochemical and Pharmaceutical Technology Department/FCF, University of São Paulo, P O Box 66083, CEP 05315-970 São Paulo, SP, Brazil.
authors.forename: Ely Vieira
authors.initials: EV
authors.lastname: Cortez
authors.forename: Adalberto
authors.initials: A
authors.lastname: Pessoa
authors.forename: Maria das Graças de Almeida
authors.initials: Md
authors.lastname: Felipe Md
authors.forename: Inês Conceição
authors.initials: IC
authors.lastname: Roberto
authors.forename: Michele
authors.initials: M
authors.lastname: Vitolo
mesh_terms: D002175:Candida / Q000201:enzymology*; D002474:Cell-Free System; D050542:D-Xylulose Reductase; D008823:Micelles; D013401:Sugar Alcohol Dehydrogenases / Q000302:isolation & purification*; D013501:Surface-Active Agents
chemical_list: D008823:Micelles; D013501:Surface-Active Agents; D013401:Sugar Alcohol Dehydrogenases; D050542:D-Xylulose Reductase