Somatic DNA recombination yielding circular DNA and deletion of a genomic region in embryonic brain.
In this study, a mouse genomic region is identified that undergoes DNA rearrangement and yields circular DNA in brain during embryogenesis. External region-directed inverse polymerase chain reaction on circular DNA extracted from late embryonic brain tissue repeatedly detected DNA of this region containing recombination joints. Wide-range genomic PCR and digestion-circularization PCR analysis showed this region underwent recombination accompanied with deletion of intervening sequences, including the circularized regions. This region was mapped by fluorescence in situ hybridization to C1 on mouse chromosome 16, where no gene and no physiological DNA rearrangement had been identified. DNA sequence in the region has segmental homology to an orthologous region on human chromosome 3q.13. These observations demonstrated somatic DNA recombination yielding genomic deletions in brain during embryogenesis.
Maeda
Toyoki
T
Department of Molecular Genetics, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Higashi-ku, Maidashi, Fukuoka 812-8582, Japan. maedat@beppu.kyushu-u.ac.jp
Chijiiwa
Yoshiharu
Y
Tsuji
Hideo
H
Sakoda
Saburo
S
Tani
Kenzaburo
K
Suzuki
Tomokazu
T
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000502:physiology*; D002875:Chromosomes / Q000235:genetics / Q000378:metabolism; D004270:DNA, Circular / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D004622:Embryo, Mammalian / Q000033:anatomy & histology / Q000502:physiology*; D017353:Gene Deletion; D015321:Gene Rearrangement; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011995:Recombination, Genetic
D004270:DNA, Circular