A novel method of Multiplexed Competitive Antibody Binning for the characterization of monoclonal antibodies.
We have developed a novel method of high-throughput Multiplexed Competitive Antibody Binning (MCAB). Using only a small amount of antibody and antigen, this method enables the sorting of a large, complex panel of monoclonal antibodies into different bins based on cross-competition for antigen binding. The MCAB assay builds on Luminex multiplexing bead-based technology to detect antibody competition. Because of its high sensitivity, the MCAB method is immediately applicable after identification of antigen-positive mAbs, providing information useful for advancing mAb candidates into further testing. The MCAB assay also can be used for sorting mAbs into binding groups after screening for functional activity.
Jia
Xiao-Chi
XC
Abgenix, Inc., 6701 Kaiser Drive, Fremont, CA 94555, USA.
Raya
Robert
R
Zhang
Li
L
Foord
Orit
O
Walker
Wynn L
WL
Gallo
Michael L
ML
Haak-Frendscho
Mary
M
Green
Larry L
LL
Davis
C Geoffrey
CG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology*; D001666:Binding Sites, Antibody / Q000276:immunology; D001667:Binding, Competitive / Q000276:immunology*; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology; D006801:Humans; D051379:Mice
D000911:Antibodies, Monoclonal; D000939:Epitopes