title: [Content determination of S-citalopram by chiral high-performance liquid chromatography].
abstract: OBJECTIVE
  To separate the enantiomers of citalopram and determine the content of S-citalopram using chiral high-performance liquid chromatography (HPLC).
  
  
  METHODS
  The chiral column CHIROBIOTIC V was used with the mobile phase using methanol-acetic acid-triethylamine (100:0.1:0.1) at the detection wavelength of 240 nm, column temperature of 20 degrees Celsius and flow rate of 1.0 ml/min.
  
  
  RESULTS
  Complete separation of the enantiomers of citalopram was achieved, and S-citalopram exhibited good linearity within the concentration range of 10 to 150 microg/ml (r=0.9991, n=5).
  
  
  CONCLUSIONS
  This method allows accurate quantification of S- and R-citalopram and is well suited for drug interaction investigations.
authors.affiliation: Department of Chemistry, First Military Medical University, Guangzhou 510515, China.
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Yang
authors.forename: Xu
authors.initials: X
authors.lastname: Liu
authors.forename: Yi-chen
authors.initials: YC
authors.lastname: Yan
authors.forename: Jiang-ping
authors.initials: JP
authors.lastname: Xu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D015283:Citalopram / Q000032:analysis*; D013237:Stereoisomerism
chemical_list: D015283:Citalopram