Studies of calmodulin-dependent regulation.
Methods are presented for purifying bovine testes calmodulin and the calmodulin-regulated plasma-membrane calcium ATPase from human erythrocytes by calcium dependent affinity chromatography. The assay of CaM Kinase II using a synthetic peptide substrate is also described.
Brandt
Paul C
PC
Department of Medical Pharmacology and Toxicology, Texas A&M University Health Science Center, College Station, TX, USA.
Vanaman
Thomas C
TC
D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000737:chemistry / Q000302:isolation & purification; D002147:Calmodulin / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002148:Calmodulin-Binding Proteins / Q000302:isolation & purification*; D002417:Cattle; D002457:Cell Extracts / Q000032:analysis; D002846:Chromatography, Affinity / Q000379:methods*; D002847:Chromatography, Agarose; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D008297:Male; D010640:Phenothiazines / Q000737:chemistry; D013737:Testis / Q000737:chemistry / Q000378:metabolism; D014020:Tissue Extracts / Q000032:analysis
D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D002457:Cell Extracts; D010640:Phenothiazines; D014020:Tissue Extracts; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D000252:Calcium-Transporting ATPases; C031637:phenothiazine