[The cloning, expression, purification and identification of SARS virus S2 gene and study on its immunological characteristics].
OBJECTIVE
To express S2 protein of SARS virus fused with Trx and then detect its reactivity to the sera from convalescent SARS patients.


METHODS
The Trx-S2 fusion protein was expressed in E.coli. After purification, the Trx-S2 fusion protein was detected by Western blot with 6 serum samples of convalescent SARS patients and 6 serum samples of healthy donors.


RESULTS
According to the SDS-PAGE analysis, the relative molecular mass (M(r)) of the Trx-S2 fusion protein is about 76 x 10(3). The fusion protein could react with all the sera from convalescent SARS patients but not with the sera from healthy donors.


CONCLUSIONS
The Trx-S2 fusion protein provides a basis for the research on its role in the course of SARS virus infection of host cells and preparation of recombinant vaccine against SARS virus.
Dong
Xue-yuan
XY
Department of Immunology, Health Science Center, Peking University, Beijing 100083, China.
Yang
Xiao-ang
XA
Li
Yan
Y
Xu
Guo-bin
GB
Feng
Zhen-ru
ZR
Wang
Yue-dan
YD
Chen
Wei-feng
WF
D000914:Antibodies, Viral / Q000032:analysis; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D006801:Humans; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D021122:Protein Subunits / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D045473:Severe acute respiratory syndrome-related coronavirus / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D044967:Serum / Q000378:metabolism; D045169:Severe Acute Respiratory Syndrome / Q000097:blood* / Q000276:immunology; D064370:Spike Glycoprotein, Coronavirus; D013879:Thioredoxins / Q000096:biosynthesis; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D014764:Viral Proteins / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification
D000914:Antibodies, Viral; D008562:Membrane Glycoproteins; D021122:Protein Subunits; D011994:Recombinant Proteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D013879:Thioredoxins