SOCS5 is expressed in primary B and T lymphoid cells but is dispensable for lymphocyte production and function.
Suppressors of cytokine signaling (SOCSs) are key regulators of cytokine-induced responses in hematopoietic as well as nonhematopoietic cells. SOCS1 and SOCS3 have been shown to modulate T-cell responses, whereas the roles of other SOCS family members in the regulation of lymphocyte function are less clear. Here, we report the generation of mice with a targeted disruption of the Socs5 gene. Socs5(-/-) mice were born in a normal Mendelian ratio and were healthy and fertile. We found that SOCS5 is expressed in primary B and T cells in wild-type mice. However, no abnormalities in the lymphocyte compartment were seen in SOCS5-deficient mice. We examined antigen- and cytokine-induced proliferative responses in B and T cells in the absence of SOCS5 and found no deviations from the responses seen in wild-type cells. Because SOCS5 has been implicated in Th1 differentiation, we also investigated the importance of SOCS5 in T helper cell responses. Unexpectedly, SOCS5-deficient CD4 T cells showed no abnormalities in Th1/Th2 differentiation and Socs5(-/-) mice showed normal resistance to infection with Leishmania major. Therefore, although SOCS5 is expressed in primary B and T cells, it appears to be dispensable for the regulation of lymphocyte function.
Brender
Christine
C
The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia.
Columbus
Ruth
R
Metcalf
Donald
D
Handman
Emanuela
E
Starr
Robyn
R
Huntington
Nick
N
Tarlinton
David
D
Ødum
Niels
N
Nicholson
Sandra E
SE
Nicola
Nicos A
NA
Hilton
Douglas J
DJ
Alexander
Warren S
WS
D000818:Animals; D001402:B-Lymphocytes / Q000737:chemistry / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation; D005260:Female; D006410:Hematopoiesis; D018320:Leishmania major / Q000276:immunology; D016773:Leishmaniasis, Cutaneous / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D018345:Mice, Knockout; D014176:Protein Biosynthesis; D011506:Proteins / Q000276:immunology*; D050826:Suppressor of Cytokine Signaling Proteins; D013601:T-Lymphocytes / Q000737:chemistry / Q000166:cytology / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000737:chemistry / Q000166:cytology
D011506:Proteins; C485811:Socs5 protein, mouse; D050826:Suppressor of Cytokine Signaling Proteins