Purification, characterization and sequence determination of BmKK4, a novel potassium channel blocker from Chinese scorpion Buthus martensi Karsch.
The scorpion neurotoxin BmKK4 was purified from the venom of the Chinese scorpion Buthus martensi Karsch by a combination of gel-filtration, ion exchange and reversed phase chromatography. The primary sequence of BmKK4 was determined using the tandem MS/MS technique and the cDNA database searching as followings: ZTQCQ SVRDC QQYCL TPDRC SYGTC YCKTT (NH(2)). BmKK4 is the first isolated member of a new subfamily alpha-KTx17 of scorpion K(+) toxins.
Zhang
Naixia
N
State Key Laboratory of Bio-organic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, PR China.
Wu
Gong
G
Wu
Houming
H
Chalmers
Michael J
MJ
Gaskell
Simon J
SJ
D000595:Amino Acid Sequence; D000818:Animals; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000737:chemistry* / Q000302:isolation & purification*; D026902:Potassium Channel Blockers / Q000737:chemistry* / Q000302:isolation & purification*; D012604:Scorpion Venoms / Q000737:chemistry*; D012605:Scorpions / Q000378:metabolism; D016415:Sequence Alignment
C495934:BmKK4 neurotoxin, Buthus martensii; D009498:Neurotoxins; D026902:Potassium Channel Blockers; D012604:Scorpion Venoms