Dispensable role for interferon-gamma in the burn-induced acute phase response: a proteomic analysis.
We examined the role of the pleiotropic cytokine interferon-gamma (IFN-gamma) in initiating the burn injury-induced acute phase response (APR). Two-dimensional (2-D) electrophoresis was used to obtain serum protein profiles from wild-type (WT) and IFN-gamma knockout mice following sham-burn or 20% burn injury. Serum 2-D images from both groups of burn-injured mice were characterized by the upregulation of a similar panel of protein spots. These included the three major murine acute phase proteins haptoglobin, serum amyloid A, and serum amyloid P, that were identified by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF)-mass spectrometry. Furthermore, the changes in the levels of these protein spots were very similar between these two groups of mice, as determined by image analysis. Other features of burn-induced APR such as a decrease in total serum protein concentration, an elevated circulation level of the cytokine interleukin-6 (IL-6), and activation of the IL-6 signal transduction protein STAT3 were also evaluated and found to be similar between wild-type and IFN-gamma knockout mice. These results suggest a dispensable role of IFN-gamma in the induction of the hepatic APR in mice following burn injury.
Duan
Xunbao
X
Center for Engineering in Medicine/Department of Surgery, Massachusetts General Hospital, Harvard Medical School and Shriners Burns Hospital, Boston 02114, USA.
Yarmush
David M
DM
Jayaraman
Arul
A
Yarmush
Martin L
ML
D000210:Acute-Phase Reaction / Q000209:etiology / Q000473:pathology*; D000818:Animals; D015153:Blotting, Western; D002056:Burns / Q000378:metabolism* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D007371:Interferon-gamma / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D013058:Mass Spectrometry; D051379:Mice; D018345:Mice, Knockout; D008954:Models, Biological; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015854:Up-Regulation
D020543:Proteome; D007371:Interferon-gamma