Muscle-nerve-muscle neurotization for the reinnervation of denervated somatic muscle.
Muscle-Nerve-Muscle (MNM) is the reinnervation of a denervated (recipient) muscle via a nerve graft inserted into the belly of an innervated (donor) muscle. MNM is studied for the reinnervation of intrinsic denervated somatic skeletal muscle by evaluating both restored muscle contractile ability and innervation state. In a rat model, muscle function is tested following MNM neurotization from an innervated (donor), extensor digitorum longus muscle to a denervated (recipient), peroneus digit quinti (PDQ) muscle. PDQ muscle cross-sections labeled for neural cell adhesion molecule protein (NCAM), a marker for fiber denervation. MNM neurotization results in the recovery of PDQ muscle force generating capacity (58% of Normal-control) and a significantly lower percentage of residual muscle fiber denervation (38% denervated) compared with the Denervated-control (79% denervated) group. MNM neurotization reinnervates 62% of the previously denervated muscle fibers in the PDQ muscle. No decrement in force capacity is observed in the donor EDL muscle. Nerve grafting for MNM neurotization may restore modest contractile function to denervated muscle and reinnervate relatively more denervated muscle fibers than the Denervated-control.
Urbanchek
Melanie G
MG
Section of Plastic Surgery, Department of Surgery, University of Michigan, Ann Arbor, MI 48109-0340, USA. melurban@umich.edu
Ganz
Diana E
DE
Aydin
Mustafa A
MA
van der Meulen
Jack H
JH
Kuzon
William M
WM
D019412:Anatomy, Cross-Sectional / Q000379:methods; D000818:Animals; D002038:Bungarotoxins / Q000378:metabolism; D004576:Electromyography / Q000379:methods; D019054:Evoked Potentials, Motor / Q000502:physiology; D007839:Functional Laterality; D007150:Immunohistochemistry / Q000379:methods; D023421:Models, Animal; D009119:Muscle Contraction / Q000502:physiology; D009121:Muscle Denervation; D018485:Muscle Fibers, Skeletal / Q000502:physiology / Q000637:transplantation; D018482:Muscle, Skeletal / Q000294:innervation* / Q000503:physiopathology*; D009416:Nerve Regeneration / Q000502:physiology*; D016067:Nerve Transfer; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D014048:Tolonium Chloride
D002038:Bungarotoxins; D019006:Neural Cell Adhesion Molecules; D014048:Tolonium Chloride