Analyzing checkpoint controls in human skin.
A short-term whole-skin organ culture model has been established to enable the investigation of cell cycle perturbations in epidermal layer cells following exposure to ultraviolet radiation (UVR). This model affords the opportunity to manipulate the growth and nutrient conditions, and to perform detailed biochemical and immunohistochemical analysis of skin cells in their normal epidermal layer microenvironment. The use of this model is described in this chapter.
Pavey
Sandra
S
Queensland Institute of Medical Research, PO Royal Brisbane Hospital, Australia.
Gabrielli
Brian G
BG
D001973:Bromodeoxyuridine / Q000494:pharmacology; D002453:Cell Cycle; D004396:Coloring Agents / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D004817:Epidermis / Q000378:metabolism; D005557:Formaldehyde / Q000378:metabolism; D018816:Genes, cdc; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D009924:Organ Culture Techniques; D012867:Skin / Q000378:metabolism*; D013997:Time Factors; D014466:Ultraviolet Rays
D004396:Coloring Agents; D003470:Culture Media; D005557:Formaldehyde; D001973:Bromodeoxyuridine