title: [Preparation and the anti-tumor activity of mutant Staphylococcal enterotoxin B].
abstract: OBJECTIVE
  To prepare mutant Staphylococcal enterotoxin B(SEB) and observe its anti-tumor activity.
  
  
  METHODS
  The expressed mutant SEB-K172E in inclusion body was denatured and renatured, and then isolated and purified. The anti-tumor activity of the mutant SEB-K172E was compared with wild-type SEB.
  
  
  RESULTS
  Renaturation and purification method of the mutant SEB-K172E was developed. The anti-tumor activity of acquired mutant protein was ten times higher than that of wild-type SEB.
  
  
  CONCLUSIONS
  The anti-tumor activity of the mutant SEB-K172E is much higher than that of wild-type SEB, suggesting that the mutant SEB-K172E may become a potential anti-tumor drug.
authors.affiliation: College of Animal Science and Technology, Northwest Science and Technology University of Agriculture and Forestry, Yangling 712100, China. jiangyq@nic.bmi.ac.cn
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Deng
authors.forename: Yu-ling
authors.initials: YL
authors.lastname: Zheng
authors.forename: Feng
authors.initials: F
authors.lastname: Yu
authors.forename: Bao-an
authors.initials: BA
authors.lastname: Ning
authors.forename: Yong-qiang
authors.initials: YQ
authors.lastname: Jiang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D004768:Enterotoxins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D019073:HT29 Cells / Q000187:drug effects; D006801:Humans; D017354:Point Mutation; D020673:Protein Renaturation; D013211:Staphylococcus aureus / Q000235:genetics; D018089:Superantigens / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D004768:Enterotoxins; D018089:Superantigens; C031818:enterotoxin B, staphylococcal