[Antibody production and its neutralization of Abeta42's cytoxicity in BALB/c mice induced by Abeta42 and its subunit vaccines].
OBJECTIVE
To explore the production of anti-Abeta42 antibody after immunization with Abeta42 and its subunit peptide vaccines.


METHODS
Seventy five male BALB/c with the age of 6 weeks were randomly divided into 5 groups, namely, control group, Abeta42 group, Abeta(36-42) group, Abeta(1-15)group and F Abeta(1-15) group. The BALB/c mice were immunized four times with PBS+MF59 adjuvant, Abeta42+MF59, Abeta(36-42)+heptalysine (MAP)+MF59, Abeta(1-15)+MAP+MF59 and Abeta(1-15)+MAP+Freud's adjuvant, respectively. The titers of specific antibodies in sera and supernatants of brain tissue homogenates from the immunized mice in every group were detected by indirect ELISA. After Abeta42, Abeta(36-42), Abeta(1-15) and F Abeta(1-15) were co-cultured together with cultured PC12 cells for 7 days, the cytotoxicity of the 3 antigen peptides to PC12 cells were determined by MTT colorimetry. In addition, after immune sera from each group were added to culture medium containing 20 mg/L Abeta42 and co-cultured with PC12 cells for 7 days, the survival rate of PC12 cells were examined by MTT assay.


RESULTS
The production of anti-Abeta42 antibodies was detected in sera of each experimental group after the second time immunization, and the titer of antibody rose with the increase of immunizing times. In addition, the anti-Abeta42 antibody with low titer was also detected in supernatants of brain tissue homogenates. The Abeta42 could reduce the survival rate of PC12 cells, whereas Abeta(36-42) and Abeta(1-15) had no obvious effect on survival rate of PC12 cells. After immune sera from 4 experimental groups and Abeta42 were co-cultured with PC12 cells, their survival rate was found improved.


CONCLUSIONS
Combination of Abeta42 and its subunit (Abeta(36-42) and Abeta(1-15)) vaccines with MF59 adjuvant can induce BALB/c mice to produce anti-Abeta42 antibody. The antibody may neutralize the cytotoxicity of Abeta42.
Hu
Jin-jia
JJ
Department of Anatomy, Preclinical Medical School, Sun Yat-Sen University, Guangzhou 510080, China.
Wang
Hua-qiao
HQ
Qu
Huai-gang
HG
Xu
Jie
J
Yao
Zhi-bin
ZB
D000276:Adjuvants, Immunologic; D016229:Amyloid beta-Peptides / Q000276:immunology* / Q000633:toxicity; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000917:Antibody Formation; D002470:Cell Survival / Q000187:drug effects*; D007106:Immune Sera / Q000096:biosynthesis / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016716:PC12 Cells; D010446:Peptide Fragments / Q000276:immunology* / Q000633:toxicity; D011136:Polysorbates; D011897:Random Allocation; D051381:Rats; D013185:Squalene; D022223:Vaccines, Subunit / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D016229:Amyloid beta-Peptides; D000906:Antibodies; D007106:Immune Sera; C089950:MF59 oil emulsion; D010446:Peptide Fragments; D011136:Polysorbates; D022223:Vaccines, Subunit; C480626:amyloid beta-protein (1-15); C075222:amyloid beta-protein (1-42); C000621368:amyloid beta-protein (36-42); D013185:Squalene