[Study of gene expression profile of chronic myeloid leukemia in chronic and blastic phases].
OBJECTIVE
To study the gene expression profile of chronic myeloid leukemia (CML) in chronic and blast phases for providing information about the potential mechanisms of blastic crisis.


METHODS
The gene expression difference of bone marrow mononuclear cells between CML in chronic and in blastic phases was examined with DNA array.


RESULTS
In the blastic phase, 68 genes were obviously up-regulated in the 1176 tested genes. Among the 68 genes, the transcription factors accounted for 23.5%, cell surface antigens 22.1%, regulation proteins 19.1% and others 35.3%. There were 17 genes expressed only in the blastic phase and 11 (16.2%) genes related to G protein.


CONCLUSIONS
Obvious difference of gene expression profile existed between chronic and blastic phases of CML. Up-regulation of gene expression is one of the characteristics of CML in blastic phase. The genes related to G protein may play an important role in the blastic transformation of CML.
Wu
Bin
B
Department of Hematology, Nanfang Hospital, the First Military Medical University, Guangzhou 510515, China.
Zhou
Shu-Yun
SY
Liu
Xiao-Li
XL
D001752:Blast Crisis / Q000235:genetics* / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male