[Study on MGMT assay and tumor individual predictable chemotherapy].
How to predict and surmount the cell resistance in tumor chemotherapy is a prompt problem. We have observed that there were close correlation among O6-Methylguanine-DNA methyltransferase (MGMT) enzyme activity, protein expression and cell resistance to alkylating agents especially to nitrosourea anti-tumor compounds by a series of experiments including cell survival, xenografts in nude mice, tumor patient biopsy and molecule biology assay. We found that those tumors with high MGMT activity and abundance of MGMT protein were resistant to alkylating agents killing effect, while those with low MGMT activity and little MGMT protein were sensitive to alkylating anti-tumor drugs. We proposed a new tactics for tumor predictable chemotherapy treated with alkylating agents based on MGMT protein detection. By means of preparing MGMT monoclone antibody, we have succeeded in developing MGMT immunohistochemistry diagnostic kit.
Zhang
Yang-Pei
YP
Institute of Blood Transfusion, Academy of Military Medical Sciences, Beijing, PR China. zhangyp@nic.bmi.ac.cn
D000477:Alkylating Agents / Q000494:pharmacology; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology; D002330:Carmustine / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology; D015376:Nimustine / Q000494:pharmacology; D019853:O(6)-Methylguanine-DNA Methyltransferase / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011933:Reagent Kits, Diagnostic
D000477:Alkylating Agents; D000970:Antineoplastic Agents; D012333:RNA, Messenger; D011933:Reagent Kits, Diagnostic; D015376:Nimustine; D019853:O(6)-Methylguanine-DNA Methyltransferase; D002330:Carmustine