title: Comparison of two beta-glucosidases for the enzymatic synthesis of beta-(1-6)-beta-(1-3)-gluco-oligosaccharides.
abstract: A domain of epiglucan was synthesized by beta-glucosidases. Two beta-glucosidases, an extracellular beta-glucosidase derived from Sclerotinia sclerotiorum grown on xylose, and a commercial lyophilized preparation of beta-glucosidase from Aspergillus niger, were used to synthesize gluco-oligosaccharides from cellobiose and, specially, beta-(1-6) branched beta-(1-3) gluco-oligosaccharides, corresponding to the structure of epiglucan. Gentiobiose, cellotriose, cellotetraose, beta-Glc-(1-3)-beta-Glc-(1-4)-Glc, beta-Glc-(1-6)-beta-Glc-(1-4)-Glc and beta-Glc-(1-6)-beta-Glc-(1-3)-Glc were synthesized from cellobiose by both enzymes. The latter compound was preferentially synthesized by the beta-glycosidase from Sclerotinia sclerotiorum. Under the best conditions, only 7 g l(-1) of beta-Glc-(1-6)-beta-Glc-(1-3)-Glc was synthesized by the beta-glycosidase from Aspergillus niger compared to 20 g l(-1) synthesized with beta-glycosidase from Sclerotinia sclerotiorum.
authors.affiliation: Unité de Génie Biologique, Institut National de Sciences Appliquées et de Technologie, BP 676,1080 Tunis Cedex, Tunisia.
authors.forename: M Issam
authors.initials: MI
authors.lastname: Smaali
authors.forename: Nicolas
authors.initials: N
authors.lastname: Michaud
authors.forename: Nejib
authors.initials: N
authors.lastname: Marzouki
authors.forename: Marie Dominique
authors.initials: MD
authors.lastname: Legoy
authors.forename: Thierry
authors.initials: T
authors.lastname: Maugard
mesh_terms: D001203:Ascomycota / Q000201:enzymology*; D001234:Aspergillus niger / Q000201:enzymology*; D002475:Cellobiose / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005936:Glucans / Q000096:biosynthesis*; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D005936:Glucans; D009844:Oligosaccharides; C033671:epiglucan; D002475:Cellobiose; D001617:beta-Glucosidase