Immunolocalization of TASK-3 (KCNK9) to a subset of cortical neurons in the rat CNS.
Tandem pore domain (2P) K channels constitute the most diverse family of K channels and are responsible for background (leak or baseline) K currents. Of the 15 human 2P K channels, TASK-1, TASK-2, and TASK-3 are uniquely sensitive to physiologic pH changes as well as being inhibited by local anesthetics and activated by volatile anesthetics. In this study polyclonal antibodies selective for TASK-3 have been used to localize its expression in the rat central nervous system (CNS). TASK-3 immunostaining was found in rat cortex, hypothalamus, and hippocampus. Double immunofluorescent studies identified a discrete population of TASK-3 expressing neurons scattered throughout cortex. Using immunogold electron microscopy TASK-3 was identified at the cell surface associated with synapses and within the intracellular synthetic compartments. These results provide a more finely detailed picture of TASK-3 expression and indicate a role for TASK-3 in modulating cerebral synaptic transmission and responses to CNS active drugs.
Callahan
Robert
R
Department of Anesthesia and Perioperative Care, University of California, 513 Parnassus Ave., Room S-261, San Francisco, CA 94143-0542, USA.
Labunskiy
Dmitriy A
DA
Logvinova
Anna
A
Abdallah
Mona
M
Liu
Canhui
C
Cotten
Joseph F
JF
Yost
C Spencer
CS
D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D006863:Hydrogen-Ion Concentration; D008297:Male; D008854:Microscopy, Electron; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D015221:Potassium Channels / Q000378:metabolism*; D024683:Potassium Channels, Tandem Pore Domain; D051381:Rats; D017207:Rats, Sprague-Dawley; D013347:Subcellular Fractions / Q000378:metabolism
C407082:Kcnk9 protein, rat; D015221:Potassium Channels; D024683:Potassium Channels, Tandem Pore Domain