title: Total oxyradical scavenging capacity of the deep-sea amphipod Eurythenes gryllus.
abstract: Environmental concern for the deep-sea ecosystem is increasing as contaminants, originating from anthropogenic activities, have been detected in deep-sea biota. However, little is known on the xenobiotics metabolising capability of deep-sea fauna. In this study, the deep-sea amphipod Eurythenes gryllus was selected as sentinel species to measure the total oxyradical scavenging capacity (TOSC). Individuals of E. gryllus were sampled at 2000 m depth in the Arctic Ocean. The TOSC assay was measured on the cytosolic fraction and the soluble fraction (3 kDa) of the digestive gland and on the cell-free haemolymph toward peroxyl, hydroxyl and peroxynitrite radicals according to the method of Winston et al. [Free Radical Biology and Medicine 24 (3) (1998) 480] and Regoli and Winston [Toxicology and Applied Pharmacology 156 (1999) 96]. These results provide the first baseline data set for total antioxidant capacity in a deep-sea amphipod.
authors.affiliation: The University Centre on Svalbard, PB 156, 9170 Longyearbyen, Norway. lionel.caus@rf.no
authors.forename: L
authors.initials: L
authors.lastname: Camus
authors.forename: B
authors.initials: B
authors.lastname: Gulliksen
mesh_terms: D033304:Amphipoda / Q000378:metabolism*; D000818:Animals; D001110:Arctic Regions; D004784:Environmental Monitoring; D016166:Free Radical Scavengers / Q000378:metabolism*; D006458:Hemolymph / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D009792:Oceans and Seas; D010545:Peroxides / Q000378:metabolism; D030421:Peroxynitrous Acid / Q000378:metabolism
chemical_list: D016166:Free Radical Scavengers; D010545:Peroxides; D030421:Peroxynitrous Acid; C049375:perhydroxyl radical; D017665:Hydroxyl Radical