[The effect of polyporus umbellatus polysaccharide on the immunosuppression property of culture supernatant of S180 cells].
OBJECTIVE
To investigate the effect of polyporus umbellatus polysaccharide (PUPS) on the immunosuppression property of tumor cell line S180 culture supernatant.


METHODS
The inhibitory effects of the culture supernatant of S180 cells in the presence or absence of PUPS on the ConA-induced mouse splenocyte proliferation, IL-2 production, killer activity and the reactivity of CTLL-2 cells to IL-2 were detected by MTT colorimetry. The effect of the culture supernatant on the IL-2Ralpha expression on murine splenocytes was detected by flow cytometry.


RESULTS
The culture supernatant of S180 cells could strongly inhibit immunity in terms of the above five indexes, while PUPS could reverse the immunosuppression.


CONCLUSIONS
PUPS can offset the immunosuppression of the supernatant from S180 cell culture, which may be mediated by down-regulating the synthesis and/or secretion of immunosuppressive substance by S180 cells.
Yang
Li-juan
LJ
Department of Immunology, Institute of Basic Medical Science, Hebei Medical University, Shijiazhuang 050017, China.
Wang
Run-tian
RT
Liu
Jing-sheng
JS
Tong
Hui
H
Deng
Yu-qing
YQ
Li
Quan-hai
QH
D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D005260:Female; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007376:Interleukin-2 / Q000096:biosynthesis; D053645:Interleukin-2 Receptor alpha Subunit; D008297:Male; D051379:Mice; D011124:Polyporaceae / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D012510:Sarcoma 180 / Q000378:metabolism* / Q000473:pathology; D013154:Spleen / Q000166:cytology / Q000378:metabolism
D017077:Culture Media, Conditioned; C508018:Il2ra protein, mouse; D007166:Immunosuppressive Agents; D007376:Interleukin-2; D053645:Interleukin-2 Receptor alpha Subunit; D011134:Polysaccharides; D015375:Receptors, Interleukin-2