Microarray-based genomic surveying of gene polymorphisms in Chlamydia trachomatis.
By comparing two fully sequenced genomes of Chlamydia trachomatis using competitive hybridization on DNA microarrays, a logarithmic correlation was demonstrated between the signal ratio of the arrays and the 75-99% range of nucleotide identities of the genes. Variable genes within 14 uncharacterized strains of C. trachomatis were identified by array analysis and verified by DNA sequencing. These genes may be crucial for understanding chlamydial virulence and pathogenesis.
Brunelle
Brian W
BW
Program in Infectious Diseases, University of California, Berkeley, CA 94720-7360, USA.
Nicholson
Tracy L
TL
Stephens
Richard S
RS
D000818:Animals; D002692:Chlamydia trachomatis / Q000235:genetics* / Q000472:pathogenicity; D004195:Disease Models, Animal; D005798:Genes, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009928:Organ Specificity / Q000235:genetics; D011014:Pneumonia / Q000382:microbiology; D011110:Polymorphism, Genetic / Q000235:genetics*; D012107:Research Design; D013045:Species Specificity; D018523:Tropism / Q000235:genetics