title: Differential effects of emphysema on skeletal muscle fibre atrophy in hamsters.
abstract: Patients afflicted with emphysema demonstrate altered peripheral skeletal muscle fibre composition and atrophy. It is unknown whether these alterations are general to all skeletal muscles independent of function, phenotype or oxidative capacity. Therefore, the purpose of this investigation was to determine whether emphysema induces alterations in muscle fibre composition or atrophy in respiratory and locomotory muscles with diverse fibre types and metabolic profiles. Fibre composition and cross-sectional area were measured in selected hindlimb muscles and diaphragm of hamsters following saline (control, n=7) or elastase (emphysema, n=15) instillation. Excised lung volume increased 145% with emphysema. Fibre composition was largely unaltered, with the exception of a 13% reduction in IIB fibres in the tibialis anterior muscle of emphysema animals. Type I fibre size was also mainly unaltered, except for a diminished cross-sectional area in plantaris muscle. However, fibre cross-sectional area of fast-twitch types IIA, IIX and/or IIB fibres was reduced in the caudal biceps femoris, vastus lateralis, tibialis anterior, gastrocnemius and plantaris muscles of emphysema animals. In contrast, there was a trend for emphysema to increase the cross-sectional area of type IIA fibres in the diaphragm. These data demonstrate that emphysema-induced atrophy primarily affects locomotory muscles, independent of phenotype or oxidative capacity.
authors.affiliation: College of Health, University of Utah, Salt Lake City, UT 84112-0920, USA. jmattson@hsc.utah.edu
authors.forename: J P
authors.initials: JP
authors.lastname: Mattson
authors.forename: M D
authors.initials: MD
authors.lastname: Delp
authors.forename: D C
authors.initials: DC
authors.lastname: Poole
mesh_terms: D000818:Animals; D001284:Atrophy; D006224:Cricetinae; D003964:Diaphragm; D006614:Hindlimb; D007150:Immunohistochemistry; D008297:Male; D008647:Mesocricetus; D018485:Muscle Fibers, Skeletal / Q000473:pathology*; D018482:Muscle, Skeletal / Q000473:pathology*; D010196:Pancreatic Elastase; D011656:Pulmonary Emphysema / Q000139:chemically induced / Q000473:pathology*
chemical_list: D010196:Pancreatic Elastase