[Role of nitric oxide--NF-kappaB signaling pathway in differentiation of human naive T lymphocytes into Th1/Th2 cells].
OBJECTIVE
To explore the role of nitric oxide (NO)--NF-kappaB signaling pathway in the differentiation of human naive T lymphocytes into Th1/Th2 cells.


METHODS
Human naive T lymphocytes were isolated from umbilical blood. Various concentrations of NO donor sodium nitroprusside (SNP), NO inhibitor NAME and NF-kappaB inhibitor PDTC were added to the culture medium to induce the differentiation of naive T cells towards Th1/Th2 cells. The expressions of intracellular cytokine IFN-gamma and IL-4 were detected by flow cytometry.


RESULTS
The treatment of SNP, NAME and PDTC made no difference on the percentage of cells expressing IFN-gamma (Th1) or IL-4 (Th2) in comparison with that of the control group (P < 0.05).


CONCLUSIONS
Signaling pathway of NO--NF-kappaB had no effect on differentiation of human naive T lymphocytes into Th1 and Th2 cells. The role of NO--NF-kappaB signaling pathway in the regulation of types 1 and 2 cytokines may occur mainly at the level of mature Th cells.
Zhang
Ning
N
Department of Respiratory Medicine, Tongji Hospital of Tongji Medical College, Huazhong University of Sciences and Technology, Wuhan 430030, China. zhangning_tj@hotmail.com
Xu
Yong-jian
YJ
Zhang
Zhen-xiang
ZX
Deng
Qun-yi
QY
Xiong
Wei-ning
WN
Liu
Xian-sheng
XS
D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000037:antagonists & inhibitors*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D011759:Pyrrolidines / Q000494:pharmacology; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects; D018417:Th1 Cells / Q000166:cytology* / Q000378:metabolism; D018418:Th2 Cells / Q000166:cytology* / Q000378:metabolism; D013859:Thiocarbamates / Q000494:pharmacology
D016328:NF-kappa B; D020030:Nitric Oxide Donors; D011759:Pyrrolidines; D013859:Thiocarbamates; D009599:Nitroprusside; C020972:pyrrolidine dithiocarbamic acid; D009569:Nitric Oxide; D007371:Interferon-gamma; D019331:NG-Nitroarginine Methyl Ester