title: Embryonic chromosomal abnormalities obtained after rescue intracytoplasmic sperm injection of 1-day-old unfertilized oocytes.
abstract: OBJECTIVE
  To study if second day intracytoplasmic sperm injection (ICSI) results in chromosomal abnormalities in the embryos.
  
  
  METHODS
  Rescue ICSI was performed on 14 metaphase II (MII) oocytes after unsuccessful conventional IVF, four were fertilized. Fluorescent in situ hybridization (FISH) was performed on these four embryos and was informative for three.
  
  
  RESULTS
  There were two tetraploid embryos, one mosaic embryo with trisomy 21, tetrasomy 18, and tetrasomy for sex chromosomes in one cell and trisomy 22 in another cell.
  
  
  CONCLUSIONS
  We discourage the use of second day ICSI due to the observed increase in chromosomal abnormalities in these embryos.
authors.affiliation: Instituto Valenciano de Infertilidad Valencia, Spain.
authors.forename: Tugce
authors.initials: T
authors.lastname: Pehlivan
authors.forename: Carmen
authors.initials: C
authors.lastname: Rubio
authors.forename: Amparo
authors.initials: A
authors.lastname: Ruiz
authors.forename: Jose
authors.initials: J
authors.lastname: Navarro
authors.forename: José
authors.initials: J
authors.lastname: Remohí
authors.forename: Antonio
authors.initials: A
authors.lastname: Pellicer
authors.forename: Carlos
authors.initials: C
authors.lastname: Simón
mesh_terms: D000328:Adult; D002869:Chromosome Aberrations; D004622:Embryo, Mammalian / Q000002:abnormalities*; D047108:Embryonic Development; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D009865:Oocytes; D011247:Pregnancy; D013048:Specimen Handling; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013094:Spermatozoa / Q000473:pathology; D013997:Time Factors; D017211:Treatment Failure