title: Activation of NF-kappaB/Rel transcription factors in human primary peripheral blood mononuclear cells by interleukin 7.
abstract: Pathways that regulate the activation of NF-kappaB/Rel transcription factors are known to include signaling through a number of cytokine receptors. Interleukin 7 (IL-7), produced by bone marrow and other stromal cells, is a key factor for differentiation and survival in the lymphoid and other compartments. We found that human recombinant IL-7 induced NF-kappaB/Rel activation, analyzed by electrophoretic mobility shift assay (EMSA), in human peripheral blood T lymphocytes from healthy donors. Induced complexes included p65 and p50 NF-kappaB/Rel subunits. These results demonstrate for the first time that IL-7 can participate in signaling leading to NF-kappaB/Rel activation.
authors.affiliation: Department of Biochemistry and Biomedical Technologies, University Federico II, via Pansini 5, I-80131 Naples, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Lamberti
authors.forename: Antonello
authors.initials: A
authors.lastname: Petrella
authors.forename: Maria
authors.initials: M
authors.lastname: Pascale
authors.forename: Maria Fiammetta
authors.initials: MF
authors.lastname: Romano
authors.forename: Rita
authors.initials: R
authors.lastname: Bisogni
authors.forename: Natalio
authors.initials: N
authors.lastname: Vita
authors.forename: Salvatore
authors.initials: S
authors.lastname: Venuta
authors.forename: Maria Caterina
authors.initials: MC
authors.lastname: Turco
mesh_terms: D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015851:Interleukin-7 / Q000235:genetics / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008213:Lymphocyte Activation; D016328:NF-kappa B / Q000378:metabolism*; D020569:Proto-Oncogene Proteins c-rel / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D015851:Interleukin-7; D016328:NF-kappa B; D020569:Proto-Oncogene Proteins c-rel; D011994:Recombinant Proteins; D014157:Transcription Factors