[Genetic variability and interrelationship of Siberian and Far Eastern larches from RAPD-analysis data].
Genetic diversity of larches from six geographically isolated regions, Tomsk, Irkutsk, Ulan-Ude (Siberia), and Blagoveshchensk, Khabarovsk, Yuzhno-Sakhalinsk (Far East) was examined by means of RAPD analysis. Tree DNA samples were compared using 457 RAPD loci (97% of which were polymorphic), identified with 17 primers of random sequences. In the samples examined, 32 to 49% of the genes were in heterozygous state, mean expected heterozygosity (Hexp) varied from 0.1373 to 0.1891, and the genetic distances (DN) for different sample pairs varied from 0.0361 to 0.1802. The main population parameters were determined for Larix sibirica Ledeb., L. gmelinni (Rupr.) Rupr., and L. kamtschatica (Rupr.) Carr. Analysis of the genetic relationships showed that L. kamtschatica was characterized by highest genetic differentiation from the other larches examined, while larches from Primorskii krai were genetically close to L. sibirica.
Kozyrenko
M M
MM
Institute of Biology and Soil Science, Russian Academy of Sciences, Vladivostok, 690022 Russia.
Artiukova
E V
EV
Reunova
G D
GD
Levina
E A
EA
Zhuravlev
Iu N
IuN
D018744:DNA, Plant; D006579:Heterozygote; D028221:Larix / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D012800:Siberia; D013045:Species Specificity
D018744:DNA, Plant