Pkc-a differentially affects rutabaga and wild-type Drosophila neuronal potassium current.
Learning and memory are defective in the Drosophila mutant rutabaga, which has a low intracellular cyclic adenosine monophosphate (cAMP) concentration. The aim of this study was to compare modulation effects of protein kinase C activator (PKC-A) on the delayed-rectifier potassium current (IKDR) in wild-type and rutabaga neurons. IKDR was measured from cultured (2 days) wild-type and rutabaga neurons. The authors examined the effects of PKC-A on IKDR in wild-type and rutabaga neurons. IKDR was measured from neurons before and after addition of PKC-A to the external solution. IKDR was smaller in rutabaga neurons (380 +/- 25 pA) than in wild-type neurons (529 +/- 44 pA). IKDR was reduced by PKC-A more in wild-type (decreasing 55 +/- 6%) than in rutabaga (decreasing 35 +/- 8%) neurons (single-cell studies). In the presence of PKC-A, there was no difference in IKDR between wild-type (229 +/- 31 pA) and rutabaga (242 +/- 26 pA) neurons (population studies). These results indicate that PKC-A differentially affects the delayed-rectifier channel in wild-type rutabaga.
Alshuaib
Waleed B
WB
Department of Physiology, Faculty of Medicine, Kuwait University, Safat, Kuwait. waleed@hsc.kuniv.edu.kw
Mathew
Mini V
MV
Hasan
Mohammed Y
MY
Fahim
Mohamed A
MA
D000818:Animals; D030801:Animals, Genetically Modified; D002478:Cells, Cultured; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism*; D004553:Electric Conductivity; D004594:Electrophysiology; D004625:Embryo, Nonmammalian; D020536:Enzyme Activators / Q000494:pharmacology*; D005838:Genotype; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D018408:Patch-Clamp Techniques / Q000379:methods; D011188:Potassium / Q000378:metabolism; D024661:Potassium Channels, Inwardly Rectifying / Q000187:drug effects / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*
D029721:Drosophila Proteins; D020536:Enzyme Activators; D024661:Potassium Channels, Inwardly Rectifying; D011493:Protein Kinase C; D011188:Potassium