[The expression of truncated YggG protein and preparation of its polyclonal antibody].
OBJECTIVE
To express truncated YggG protein (TYP) in Escherichia coli and to prepare anti-TYPE antibody.


METHODS
Truncated yggg gene was amplified by PCR from the plasmid containing full length yggg gene and was cloned into the expression vector pDH2. The expression of TYP was achieved by thermal induction. Antiserum was prepared by immunizing rabbit with TYP,The titer and specificity of polyclonal antibody were detected by Western blot.


RESULTS
Thin-layer scan analysis showed that TYP accounted for about 37.1% of total bacterial protein. The antiserum was about 1:2,000 in titier and highly specific. Full-length rggG protein could also be recognized by the antiserum.


CONCLUSIONS
The TYP was highly expressed, and specific anti-TYP antiserum was prepared successfully.
Huang
Yong
Y
Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710032, China.
Chen
Su-min
SM
Chen
Nan-chun
NC
Li
Jie
J
Zhang
Bin
B
Zhang
Xiao-nan
XN
D000818:Animals; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D005798:Genes, Bacterial; D005822:Genetic Vectors; D007106:Immune Sera / Q000096:biosynthesis* / Q000276:immunology; D010957:Plasmids; D011817:Rabbits; D014169:Transformation, Bacterial
D029968:Escherichia coli Proteins; D007106:Immune Sera