title: Molecular diversity of Epstein-Barr virus IgG and IgA antibody responses in nasopharyngeal carcinoma: a comparison of Indonesian, Chinese, and European subjects.
abstract: Epstein-Barr virus (EBV)-specific immunoblot analysis was used to reveal the molecular diversity of immunoglobulin (Ig) G and IgA antibody responses against Epstein-Barr nuclear antigen (EBNA), early antigen (EA), and viral capsid antigen (VCA) in serum samples from patients with nasopharyngeal carcinoma (NPC) and control subjects, by use of immunofluorescence assay (IFA). Control donors (n=150) showed IgG responses to few EBV proteins--VCA-p18, VCA-p40, EBNA1, and Zebra--and sporadically weak IgA reactivity to EBNA1 and VCA-p18. Patients with NPC stage 1 (n=6) had similar response patterns. Patients with NPC stage 2-4 (n=132) showed significantly more diverse IgG and IgA responses to EA and VCA proteins--VCA-p18/-p40, EBNA1, Z-encoded broadly reactive activator, and EAd-p47/54, -DNAse, -thymidine kinase, and -p138. No correlation was found between IFA titers and the number of EBV proteins recognized by IgG or IgA. Our results reveal dissimilarity between EBV polypeptides recognized by IgG and IgA antibodies, which suggests independent B cell triggering events.
authors.affiliation: Gadjah Mada University, Yogyakarta, Indonesia.
authors.forename: Jajah
authors.initials: J
authors.lastname: Fachiroh
authors.forename: Tabitha
authors.initials: T
authors.lastname: Schouten
authors.forename: Bambang
authors.initials: B
authors.lastname: Hariwiyanto
authors.forename: Dewi K
authors.initials: DK
authors.lastname: Paramita
authors.forename: Ahmad
authors.initials: A
authors.lastname: Harijadi
authors.forename: Sofia M
authors.initials: SM
authors.lastname: Haryana
authors.forename: Mun H
authors.initials: MH
authors.lastname: Ng
authors.forename: Jaap M
authors.initials: JM
authors.lastname: Middeldorp
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology; D044466:Asian People; D036022:Capsid Proteins / Q000276:immunology; D002277:Carcinoma / Q000453:epidemiology / Q000208:ethnology / Q000276:immunology / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay; D020031:Epstein-Barr Virus Infections / Q000453:epidemiology / Q000276:immunology / Q000821:virology; D019309:Epstein-Barr Virus Nuclear Antigens / Q000276:immunology; D005060:Europe / Q000453:epidemiology; D014644:Genetic Variation; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology*; D006723:Hong Kong / Q000453:epidemiology; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood*; D007214:Indonesia / Q000453:epidemiology; D009303:Nasopharyngeal Neoplasms / Q000453:epidemiology / Q000208:ethnology / Q000276:immunology / Q000821:virology*; D044465:White People
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D019309:Epstein-Barr Virus Nuclear Antigens; C041788:Epstein-Barr viral capsid antigen; C033305:Epstein-Barr virus early antigen; D007070:Immunoglobulin A; D007074:Immunoglobulin G; C417029:EBV-encoded nuclear antigen 1