Large dense-core vesicle exocytosis in pancreatic beta-cells monitored by capacitance measurements.
This article discusses the currently used methodologies for monitoring exocytosis as changes in cell capacitance. Details are given on composition of solutions, experimental protocols, and how the observed responses can be interpreted physiologically. The concepts are illustrated by examples from our own work on insulin-releasing pancreatic beta-cells. Finally, we consider the feasibility of applying capacitance measurements to endocrine cells in intact pancreatic islets, where the cells are electrically coupled to each other.
Kanno
Takahiro
T
Department of Physiology, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan.
Ma
Xiasong
X
Barg
Sebastian
S
Eliasson
Lena
L
Galvanovskis
Juris
J
Göpel
Sven
S
Larsson
Max
M
Renström
Erik
E
Rorsman
Patrik
P
D000818:Animals; D003594:Cytoplasmic Granules / Q000378:metabolism* / Q000648:ultrastructure; D005089:Exocytosis / Q000502:physiology*; D007515:Islets of Langerhans / Q000378:metabolism*; D013572:Synaptic Vesicles / Q000378:metabolism*