Gypsy-like retrotransposons in Pyrenophora: an abundant and informative class of molecular markers.
This paper describes the development of S-SAP (sequence-specific amplified polymorphism) using a primer derived from the LTR (long terminal repeat) of the Pyggy retrotransposon isolated from Pyrenophora graminea. Fragments were amplified by S-SAP from different Pyrenophora spp., indicating the presence of Pyggy-like sequences in these genomes. The bands were highly polymorphic between isolates and the number of bands differed by as much as 10-fold between species, demonstrating the potential of this method for genetic analysis in fungi. The phylogenetic relationship among the isolates as deduced using S-SAP data is presented, and shows evidence of genetic exchange between P. graminea and P. teres.
Taylor
Emily J A
EJ
Molecular Research Group, NIAB, Cambridge, UK.
Konstantinova
Pavlina
P
Leigh
Fiona
F
Bates
Jayne A
JA
Lee
David
D
D001203:Ascomycota / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005819:Genetic Markers; D018626:Retroelements
D017931:DNA Primers; D004271:DNA, Fungal; D005819:Genetic Markers; D018626:Retroelements