2-Ureidoquinoline: a useful molecular element for stabilizing single cytosine and thymine bulges.
We have demonstrated that aromatic heterocycles having hydrogen-bonding surfaces complementary to those of nucleotide bases are effective molecular elements for the binding to single nucleotide bulges and base mismatches. We here report that a new molecule, 2-ureidoquinoline having an alignment of hydrogen-bonding groups in the order of acceptor-donor-donor stabilizes single cytosine and thymine bulges in duplex DNAs. Furthermore, a dimeric form of 2-ureidoquinoline stabilizes cytosine-cytosine and cytosine-thymine mismatches.
Kobori
Akio
A
PRESTO, Japan Science and Technology Agency, Kyoto 615-8510, Japan.
Murase
Takashi
T
Suda
Hitoshi
H
Saito
Isao
I
Nakatani
Kazuhiko
K
D020137:Base Pair Mismatch; D001483:Base Sequence; D001665:Binding Sites; D003596:Cytosine / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D019281:Dimerization; D006860:Hydrogen Bonding; D009287:Naphthyridines / Q000737:chemistry; D009690:Nucleic Acid Conformation; D011084:Polycyclic Aromatic Hydrocarbons / Q000494:pharmacology; D011804:Quinolines / Q000737:chemistry*; D013941:Thymine / Q000737:chemistry*
C495733:2-ureidoquinoline; D009287:Naphthyridines; D011084:Polycyclic Aromatic Hydrocarbons; D011804:Quinolines; D003596:Cytosine; D004247:DNA; D013941:Thymine