title: Nigrostriatal modifications after vanadium inhalation: an immunocytochemical and cytological approach.
abstract: Vanadium (V) has increased in the air as a component of suspended particles originated from fuel combustion. In this report, a model of inhaled V in mice was implemented to identify the effect that V has in the corpus striatum and substantia nigra, structures with high concentrations of dopamine and scarce antioxidants burden. Mice inhaled 0.02 M V2O5 1 h twice a week and were sacrificed at points from 1 to 8 weeks after inhalation, perfused, and processed for Golgi method and for tyroxine hidroxylase (TH) inmunocytochemistry. Cytological analysis consisted in counting the number of dendritic spines in 20 medium-size spiny neurons and the number of TH immunoreactive neurons in the substatia nigra pars compacta. Dendritic spine density decreased drastically after V exposure; the same was observed with the TH-positive neurons, which decreased in a time-dependent mode. No previous morphological studies about V and nervous system have been reported. The decrease in spine density and in TH-positive neurons might have functional repercussions that should be studied because the trend of this element in the atmosphere is to increase.
authors.affiliation: FES Izatacala, Neurociencias, National University of Mexico, México City.
authors.forename: M R
authors.initials: MR
authors.lastname: Avila-Costa
authors.forename: E
authors.initials: E
authors.lastname: Montiel Flores
authors.forename: L
authors.initials: L
authors.lastname: Colin-Barenque
authors.forename: J L
authors.initials: JL
authors.lastname: Ordoñez
authors.forename: A L
authors.initials: AL
authors.lastname: Gutiérrez
authors.forename: H G
authors.initials: HG
authors.lastname: Niño-Cabrera
authors.forename: P
authors.initials: P
authors.lastname: Mussali-Galante
authors.forename: T I
authors.initials: TI
authors.lastname: Fortoul
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000473:pathology*; D003712:Dendrites / Q000187:drug effects; D008297:Male; D051379:Mice; D009474:Neurons / Q000187:drug effects; D009498:Neurotoxins / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity; D013378:Substantia Nigra / Q000187:drug effects / Q000473:pathology*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D014639:Vanadium / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity*
chemical_list: D009498:Neurotoxins; D014639:Vanadium; D014446:Tyrosine 3-Monooxygenase