Evaluation of the methicillin-resistant Staphylococcus aureus (MRSA)-Screen latex agglutination test for detection of MRSA of animal origin.
Methicillin (oxacillin)-resistant staphylococci (MRS) have emerged as major clinical and epidemiological pathogens, and there have been frequent reports of MRS infections in the veterinary field. The MRSA-Screen latex agglutination test (Denka Seiken Co., Ltd., Tokyo, Japan) was compared with an oxacillin agar screen test, MIC determination, and mecA PCR assay, the "gold standard." In an analysis of 15 mecA-positive and 48 mecA-negative S. aureus animal isolates, as well as 9 mecA-positive and 147 mecA-negative, coagulase-negative staphylococcal animal isolates, the latex agglutination test surpassed the widely used oxacillin agar screen method and MIC determination, with a sensitivity and a specificity of 100%. The MRSA-Screen test is a reliable and rapid method of detecting MRS in the veterinary field.
Lee
John H
JH
College of Veterinary Medicine, Chonbuk National University, Chonju 561-756, South Korea. johnhlee@chonbuk.ac.kr
Jeong
Jae-Myung
JM
Park
Yong-Ho
YH
Choi
Sung-Sun
SS
Kim
Yong-Hwan
YH
Chae
Joon-Seok
JS
Moon
Jin-San
JS
Park
Hyun
H
Kim
Shin
S
Eo
Seong-Kug
SK
D000818:Animals; D002417:Cattle; D002645:Chickens; D007841:Latex Fixation Tests; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D013211:Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification*; D013552:Swine