title: Wallerian degeneration after crush or chronic constriction injury of rodent sciatic nerve is associated with a depletion of endoneurial interleukin-10 protein.
abstract: We used enzyme-linked immunoassay (ELISA), immunohistochemistry (IHC), and quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) to determine whether interleukin (IL)-10 protein is changed after unilateral crush or chronic constriction injury (CCI) of mouse or rat sciatic nerve and whether IL-10 protein and mRNA are differentially regulated. In the mouse sciatic nerve, IL-10 protein declined rapidly to 10-20% of baseline early after crush or CCI, while the IL10 mRNA was up-regulated with a maximum on Days 1 and 3. In the rat sciatic nerve, IL-10 protein was significantly reduced on Day 3 after CCI, and IL-10 mRNA was up-regulated in both models. These results suggest that changes of the local cytokine network during wallerian degeneration include an early deficiency of the antiinflammatory cytokine IL-10 despite up-regulation at the mRNA level.
authors.affiliation: Neurologische Universitätsklinik Würzburg, Germany.
authors.forename: Annette
authors.initials: A
authors.lastname: George
authors.forename: Christoph
authors.initials: C
authors.lastname: Kleinschnitz
authors.forename: Marek
authors.initials: M
authors.lastname: Zelenka
authors.forename: Jörg
authors.initials: J
authors.lastname: Brinkhoff
authors.forename: Guido
authors.initials: G
authors.lastname: Stoll
authors.forename: Claudia
authors.initials: C
authors.lastname: Sommer
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism / Q000473:pathology; D002908:Chronic Disease; D004195:Disease Models, Animal; D018450:Disease Progression; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D007249:Inflammation / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D016753:Interleukin-10 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D008026:Ligation; D051379:Mice; D008810:Mice, Inbred C57BL; D009409:Nerve Crush; D010525:Peripheral Nerves / Q000166:cytology / Q000378:metabolism*; D010523:Peripheral Nervous System Diseases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000235:genetics; D012584:Sciatic Nerve / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D020426:Sciatic Neuropathy / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015854:Up-Regulation / Q000235:genetics; D014855:Wallerian Degeneration / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D016753:Interleukin-10