title: Quantitative analysis of free ubiquitin and multi-ubiquitin chain in colorectal cancer.
abstract: Accumulation of ubiquitin (Ub) has been documented in various cancers. The levels of the two forms of Ub, i.e. free ubiquitin (FUb) and multi-ubiquitin chain (MUC) were measured in colorectal cancers and in matched normal colonic tissues in 43 patients. When compared to normal colonic tissues, the levels of both FUb and MUC were significantly higher in the cancerous tissues (P < 0.01, respectively). The level of FUb was related to the depth of invasion (P < 0.05). The level of MUC was related to blood vessel invasion and lymphatic vessel invasion (P < 0.05). The levels of FUb and MUC had no correlation with histological grade, lymph node metastasis, liver metastasis or relapse-free survival. We conclude that the levels of FUb and MUC were up-regulated in colorectal cancer and were correlated with the pathological findings.
authors.affiliation: Department of Surgery, Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan. ishibashi@jikei.ac.jp
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Ishibashi
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Hanyu
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Yanai
authors.forename: Kenichi
authors.initials: K
authors.lastname: Tashiro
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Usuba
authors.forename: Shuichi
authors.initials: S
authors.lastname: Iwabuchi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Takahashi
authors.forename: Koji
authors.initials: K
authors.lastname: Takada
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ohkawa
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Urashima
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Yanaga
mesh_terms: D000328:Adult; D000368:Aged; D003106:Colon / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011863:Radioimmunoassay; D025801:Ubiquitin / Q000378:metabolism*
chemical_list: D025801:Ubiquitin