[In vitro and in vivo study on proliferation of adnoid cystic carcinoma cell lines after nm23-h1. Introduction].
OBJECTIVE
To study the effect of nm23-h1 transfection on proliferation characteristics of adnoid carcinoma cell lines in vitro and in vivo.


METHODS
In vitro ACC-M cell lines were incubated after putative anti-metastatic gene nm23-h1 was introduced into the cells with the help of G418 selective incubation base. The ACC-M cells were transplanted into 10 BALB/C nude mices subcutaneously and non-transfected cell lines were taken as control. Immunohisto chemistry and Ki67 antibody were employed to study the proliferation character of cell crawling pieces and paraffin-bedded slice, meanwhile, the solid tumor of both groups were prepared for flow cytometry(FCM).


RESULTS
Transfected cells grew slower than non-transfected cells and this trend became more obvious as passages passed on. In vitro the expression of Ki67 of transfected cells was little stronger than non-transfected cells, while the expression of Ki67 in solid slices was almost negative in both groups. Transfected cells presented slower growth than non-transfected cells in the early stage (2 weeks) and 2 weeks later there was no obvious difference in size(P > 0.05). FCM value accorded well with the result.


CONCLUSIONS
Introduction of nm23-h1 into the ACC-M cell lines may have transient inhibitory effects on its proliferation.
Li
Wen
W
Dept. of Otolaryngology, West China Hospital, Sichuan University, Chengdu 610041, China.
Wen
Yu-ming
YM
Wang
Li-hong
LH
Yang
Zhan
Z
Zhang
Rui-lin
RL
D000818:Animals; D000906:Antibodies / Q000032:analysis; D003528:Carcinoma, Adenoid Cystic / Q000235:genetics / Q000473:pathology*; D002455:Cell Division; D045744:Cell Line, Tumor; D005260:Female; D015316:Genetic Therapy; D019394:Ki-67 Antigen / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000235:genetics / Q000473:pathology*; D054778:NM23 Nucleoside Diphosphate Kinases; D009368:Neoplasm Transplantation; D009701:Nucleoside-Diphosphate Kinase; D011506:Proteins / Q000235:genetics*; D014162:Transfection
D000906:Antibodies; D019394:Ki-67 Antigen; D054778:NM23 Nucleoside Diphosphate Kinases; D011506:Proteins; C517939:Nme1 protein, mouse; D009701:Nucleoside-Diphosphate Kinase