title: [Transfection of human endostatin gene with lipofectamin and the expression of hES protein in Tca8113 cell].
abstract: OBJECTIVE
  The purpose of this study was to establish transfergeneic Tca8113 cell and evaluate the expression of human endostatin (hES) gene in the cell colone in vitro.
  
  
  METHODS
  To transfect hES gene into Tca8113 cells, lipofectamin was complexed with plasmid encoding hES gene, and blasticidin S antibiotic was adopted to select Tca8113--hES cell clone. Immunohistochemistry S-P method was adopted to detect the expression of hES in the transfergenic Tca8113 cell in vitro.
  
  
  RESULTS
  Transfected by hES, the transfergenic Tca8113 cells could grow and proliferate in RPMI--1640 culture medium containing blasticidin S antibiotic. The expression rate of hES reached 100%.
  
  
  CONCLUSIONS
  hES gene can express in hES-transfected Tca8113 cell in vitro.
authors.affiliation: Dept. of Oral and Maxillofacial Surgery, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China.
authors.forename: Chao-bin
authors.initials: CB
authors.lastname: Pan
authors.forename: Hong-zhang
authors.initials: HZ
authors.lastname: Huang
authors.forename: Jian-guang
authors.initials: JG
authors.lastname: Wang
authors.forename: Jin-song
authors.initials: JS
authors.lastname: Hou
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002455:Cell Division; D003001:Cloning, Molecular; D043169:Endostatins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008055:Lipids / Q000494:pharmacology; D014062:Tongue Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D043169:Endostatins; D008055:Lipids; C086724:Lipofectamine