Use of suppressive subtractive hybridization and cDNA arrays to discover patterns of altered gene expression in the liver of dihydrotestosterone and 11-ketotestosterone exposed adult male largemouth bass (Micropterus salmoides).
In this study male largemouth bass (LMB) were exposed to the naturally occurring androgens, dihydrotestosterone (DHT) or 11-ketotestosterone (11-KT) in order to identify genes that are differentially regulated by these steroid hormones. Using subtractive hybridization on livers of fish treated with DHT against vehicle control, many novel LMB genes were cloned. These genes were added to our gene library and arrayed. Six genes were up-regulated and five were down-regulated by both androgens. But, each androgen also regulated specific genes. One gene that was identified as a potential androgen marker was spermidine-spermine-N(1)-acetyltransferase that was up-regulated by both androgens. Determining which genes are responsive to natural androgens will help to identify biochemical pathways that are impacted.
Blum
Jason L
JL
Interdisciplinary Program for Biomedical Research, Department of Pharmacology, University of Florida, Gainesville, FL 32610, USA.
Knoebl
Iris
I
Larkin
Patrick
P
Kroll
Kevin J
KJ
Denslow
Nancy D
ND
D000123:Acetyltransferases / Q000378:metabolism; D000818:Animals; D001492:Bass / Q000235:genetics*; D013196:Dihydrotestosterone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D015723:Gene Library; D008099:Liver / Q000378:metabolism; D009693:Nucleic Acid Hybridization / Q000379:methods; D013739:Testosterone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000099228:Diamine N-Acetyltransferase
D000123:Acetyltransferases; D013196:Dihydrotestosterone; D013739:Testosterone; D000099228:Diamine N-Acetyltransferase; C003600:11-ketotestosterone