[The mechanism for up-regulation of HLA-I expression on HepG2 cells by HBV].
OBJECTIVE
To explore the mechanism of up-regulation of HLA-I expression on HepG2 cells by wild type (WT) and nucleocapsid mutants(L97 and V60) of hepatitis B virus (HBV).


METHODS
The HBV-stable expression vectors EBO-WT, EBO-L97 and EBO-V60 were transfected into HepG2 cells via the liposome mediation, respectively. The cells were assayed by semi-quantitative RT-PCR for HLA-A gene and antigen presentation-related genes LMP2, TAP1, and tapasin mRNA expression. Western blot was applied for analysis of HLA-I protein expression in the cells.


RESULTS
HepG2 cells transfected by the 3 HBV expression vectors expressed HLA-A and TAP1, while there was no expression of HLA-A and only marginal expression of TAP1 in HepG2 cells transfected by control vector EBO. The expression level of HLA-A in the transfected cells decreased successively in the order of EBO-L97, EBO-WT and EBO-V60. There was no significant difference in the expression level of TAP1 between HepG2 cells transfected by the 3 HBV expression vectors. No detectable expression of LMP2 and tapasin was observed for all transfected cells.


CONCLUSIONS
HBV can induce the expression of HLA-I molecule and TAP1 in HepG2 cells.
Chen
Wei-hong
WH
Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. chenwhong101@163.com
Ding
Jin
J
Gong
Wei-dong
WD
Wang
Jiu-ping
JP
Pan
Lei
L
Zhang
Yan
Y
Hong
Sha
S
Zhou
Yong-xing
YX
D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D002460:Cell Line; D005786:Gene Expression Regulation; D015234:HLA-A Antigens / Q000235:genetics; D006515:Hepatitis B virus / Q000502:physiology*; D015395:Histocompatibility Antigens Class I / Q000032:analysis / Q000235:genetics*; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D014162:Transfection; D015854:Up-Regulation
D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters; D015234:HLA-A Antigens; D015395:Histocompatibility Antigens Class I; D012333:RNA, Messenger; C073119:TAP1 protein, human