title: Molecular identification of SGPP-5, a novel pacifastin-like peptide precursor in the desert locust.
abstract: Recently, a new serine protease inhibitor family has been described in arthropods. Eight members were purified from locusts and 13 peptides have been identified by cDNA cloning. The peptides share a conserved cysteine array (Cys-Xaa(9-12)-Cys-Asn-Xaa-Cys-Xaa-Cys-Xaa(2-3)-Gly-Xaa(3-6)-Cys-Thr-Xaa(3)-Cys) with nine inhibitory domains (PLDs) of the light chain of the crayfish protease inhibitor, pacifastin. A molecular identification of a pacifastin-related precursor (SGPP-5) with three novel PLD-related peptides is presented in this study. This is a first report, identifying the presence of a SGPP-transcript in the brain, fore- and hindgut, including a 100-fold difference in fat body SGPP-transcript level of male as compared with female locust.
authors.affiliation: Laboratory of Developmental Physiology, Genomics and Proteomics, K U Leuven, Naamsestraat 59, B-3000 Leuven, Belgium. Gert.simonet@bio.kuleuven.ac.be
authors.forename: Gert
authors.initials: G
authors.lastname: Simonet
authors.forename: Ilse
authors.initials: I
authors.lastname: Claeys
authors.forename: Sofie
authors.initials: S
authors.lastname: Van Soest
authors.forename: Bert
authors.initials: B
authors.lastname: Breugelmans
authors.forename: Vanessa
authors.initials: V
authors.lastname: Franssens
authors.forename: Arnold
authors.initials: A
authors.lastname: De Loof
authors.forename: Jozef
authors.initials: J
authors.lastname: Vanden Broeck
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005260:Female; D015870:Gene Expression; D006110:Grasshoppers / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D011498:Protein Precursors / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment; D015842:Serine Proteinase Inhibitors / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D010455:Peptides; D011498:Protein Precursors; D011506:Proteins; D012333:RNA, Messenger; D015842:Serine Proteinase Inhibitors; C106709:pacifastin