[Study on the inducible expression of toll-like receptors in human bladder cell line].
OBJECTIVE
To investigate and compare the expression of Toll-like receptors (TLRs) in human bladder cells under the stimulus of Escherichia coli (E. coli) versus that under the stimulus of Bacillus Calmette-Guérin (BCG).


METHODS
Human bladder cancer cell line T24 was cultured for 1 hour under the stimuli of various doses (bacillus-cell ratio) of E. coli and BCG respectively. The levels of expression of TLR-2 and TLR-4 mRNA in T24 cells were assessed by RT-PCR method.


RESULTS
By comparison with control, there was no difference observed on the expression of either TLR-2 or TLR-4 in T24 cells under the stimulus of E. coli. The expression level of either TLR-2 or TLR-4 was increased under the stimulus of BCG in a dose-dependent manner. The effects reached the highest level when the dose of BCG was 10 bacilli per cell.


CONCLUSIONS
There exist different expression patterns of TLRs in bladder transitional cells under the different stimuli of E. coli and BCG respectively.
Li
Xiang
X
Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.
Gong
Zhi-yong
ZY
Shi
Ming
M
Wei
Qiang
Q
Li
Hong
H
Yang
Yu-ru
YR
D001500:BCG Vaccine / Q000276:immunology / Q000494:pharmacology*; D002295:Carcinoma, Transitional Cell / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D004926:Escherichia coli / Q000276:immunology; D006801:Humans; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology
D001500:BCG Vaccine; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; C495342:TLR2 protein, human; C495345:TLR4 protein, human; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors