Surface plasmon resonance immunosensor using self-assembled protein G for the detection of Salmonella paratyphi.
A surface plasmon resonance (SPR) based immunosensor using self-assembled protein G was developed for the detection of Salmonella paratyphi. In order to endow a solid substrate binding affinity to protein G, the free amine (-NH2) of protein G was substituted into thiol (-SH) using 2-iminothiolane. Thus, self-assembled protein G was fabricated on gold (Au) substrate. The formation of protein G layer on Au surface, and the binding of antibody and antigen in series were confirmed by SPR spectroscopy. The surface morphology analysis of the protein G layer on Au surface was performed by atomic force microscope (AFM). Consequently, an immunosensor based on SPR for the detection of S. paratyphi using self-assembled protein G was developed with a detection range of 10(2)-10(7) CFU/ml. The current fabrication technique of a SPR immunosensor for the detection of S. paratyphi could be applied to construct other immnosensors or protein chips.
Oh
Byung-Keun
BK
Department of Chemical and Biomolecular Engineering, Sogang University, 1 Shinsu-dong, Mapo-gu, Seoul 121-742, South Korea.
Lee
Woochang
W
Kim
Young-Kee
YK
Lee
Won Hong
WH
Choi
Jeong-Woo
JW
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D015169:Colony Count, Microbial / Q000295:instrumentation* / Q000379:methods; D003460:Crystallization / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D046912:Multiprotein Complexes / Q000737:chemistry; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D012482:Salmonella paratyphi A / Q000276:immunology / Q000302:isolation & purification*; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods
D020099:Coated Materials, Biocompatible; C029152:G-substrate; D046912:Multiprotein Complexes; D009419:Nerve Tissue Proteins