Removal of promoter nucleosomes by disassembly rather than sliding in vivo.
Previous work demonstrated the removal of nucleosomes from the PHO5 promoter upon transcriptional activation in yeast. Removal could occur by nucleosome disassembly or by sliding of nucleosomes away from the promoter. We have now activated the PHO5 promoter on chromatin circles following excision from the chromosomal locus. Whereas sliding would conserve the number of nucleosomes on the circle, we found that the number was diminished, demonstrating chromatin remodeling by nucleosome disassembly.
Boeger
Hinrich
H
Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Griesenbeck
Joachim
J
Strattan
J Seth
JS
Kornberg
Roger D
RD
D000135:Acid Phosphatase; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly / Q000235:genetics*; D009707:Nucleosomes / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D014158:Transcription, Genetic / Q000235:genetics; D015533:Transcriptional Activation / Q000235:genetics*
D002352:Carrier Proteins; D002843:Chromatin; D009707:Nucleosomes; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; C026989:thiamine-binding protein; D000135:Acid Phosphatase; C488913:PHO3 protein, S cerevisiae; C488916:PHO5 protein, S cerevisiae