title: Assaying cytochrome C translocation during apoptosis.
abstract: Translocation of proteins from the mitochondrial intermembrane space to the cytoplasm is a critical event during apoptosis. There are several methods for assaying this event cited in the literature. In this chapter, we highlight separation of cytosolic and mitochondrial fractions of cultured cells using digitonin as the method for measuring cytochrome c release that, in our hands has been the simplest and most reproducible.
authors.affiliation: Cancer Immunology, Research Division, Peter MacCallum Cancer Center, Melbourne, Australia.
authors.forename: Nigel J
authors.initials: NJ
authors.lastname: Waterhouse
authors.forename: Rohan
authors.initials: R
authors.lastname: Steel
authors.forename: Ruth
authors.initials: R
authors.lastname: Kluck
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Trapani
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D045304:Cytochromes c / Q000032:analysis / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology / Q000378:metabolism; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D007425:Intracellular Membranes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D045304:Cytochromes c