Separation and characterization of phenolic compounds in fennel (Foeniculum vulgare) using liquid chromatography-negative electrospray ionization tandem mass spectrometry.
Liquid chromatography (LC) diode array detection (DAD) coupled to negative electrospray ionization (ESI) tandem mass spectrometry (MS/MS) was used for the rapid and sensitive identification of water-soluble phenolic compounds in fennel waste. The plant material was first extracted and then chromatographed on Sephadex LH-20 to afford seven fractions, each of them being subjected to LC-MS analysis. Identification of the compounds was carried out by interpretation of UV, MS, and MS/MS spectra. Forty-two phenolic substances were identified, 27 of which had not previously been reported in fennel, including hydroxycinnamic acid derivatives, flavonoid glycosides, and flavonoid aglycons.
Parejo
Irene
I
Departament de Productes Naturals, Biologia Vegetal i Edafologia, Facultat de Farmàcia, Universitat de Barcelona, Avinguda Joan XXIII s/n, 08028 Barcelona, Catalunya, Spain.
Jauregui
Olga
O
Sánchez-Rabaneda
Ferran
F
Viladomat
Francesc
F
Bastida
Jaume
J
Codina
Carles
C
D005591:Chemical Fractionation; D002853:Chromatography, Liquid; D003373:Coumaric Acids / Q000032:analysis / Q000302:isolation & purification; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification; D030001:Foeniculum / Q000737:chemistry*; D006027:Glycosides / Q000032:analysis / Q000302:isolation & purification; D010636:Phenols / Q000032:analysis / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization
D003373:Coumaric Acids; D005419:Flavonoids; D006027:Glycosides; D010636:Phenols