title: Gypsy-like retrotransposons in Pyrenophora: an abundant and informative class of molecular markers.
abstract: This paper describes the development of S-SAP (sequence-specific amplified polymorphism) using a primer derived from the LTR (long terminal repeat) of the Pyggy retrotransposon isolated from Pyrenophora graminea. Fragments were amplified by S-SAP from different Pyrenophora spp., indicating the presence of Pyggy-like sequences in these genomes. The bands were highly polymorphic between isolates and the number of bands differed by as much as 10-fold between species, demonstrating the potential of this method for genetic analysis in fungi. The phylogenetic relationship among the isolates as deduced using S-SAP data is presented, and shows evidence of genetic exchange between P. graminea and P. teres.
authors.affiliation: Molecular Research Group, NIAB, Cambridge, UK.
authors.forename: Emily J A
authors.initials: EJ
authors.lastname: Taylor
authors.forename: Pavlina
authors.initials: P
authors.lastname: Konstantinova
authors.forename: Fiona
authors.initials: F
authors.lastname: Leigh
authors.forename: Jayne A
authors.initials: JA
authors.lastname: Bates
authors.forename: David
authors.initials: D
authors.lastname: Lee
mesh_terms: D001203:Ascomycota / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005819:Genetic Markers; D018626:Retroelements
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; D005819:Genetic Markers; D018626:Retroelements