title: Selectivity of quadruplex DNA stationary phases toward amino acids in homodipeptides and alanyl dipeptides.
abstract: Series of dipeptides, including homodipeptides and alanyl dipeptides, were separated using quadruplex (G-quartet) DNA stationary phases in open-tubular capillary electrochromatography (OTCEC). The stationary phases were constructed by covalently attaching the DNA oligonucleotides to the inner capillary surface. Three different G-quartet forming oligonucleotides were investigated: the two-plane G-quartet forming thrombin-binding aptamer, the four-plane analogue of the thrombin-binding aptamer, and a two-plane oligonucleotide identical to the thrombin-binding aptamer except for the replacement of the guanine by thymine in the central bridging loop of the G-quartet structure. Results were compared with results obtained using capillary electrophoresis on a bare capillary and OTCEC using an oligonucleotide with the same base composition as the thrombin-binding aptamer but in a different sequence that does not allow G-quartet formation as the stationary phase.
authors.affiliation: Department of Chemistry, P. M. Gross Chemical Laboratory, Duke University, Durham, NC, USA.
authors.forename: Trang U
authors.initials: TU
authors.lastname: Vo
authors.forename: Linda B
authors.initials: LB
authors.lastname: McGown
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D052157:Aptamers, Nucleotide; D002845:Chromatography; D004247:DNA / Q000737:chemistry*; D004151:Dipeptides / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D054856:G-Quadruplexes; D057927:Hydrophobic and Hydrophilic Interactions; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D011485:Protein Binding; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D052157:Aptamers, Nucleotide; D004151:Dipeptides; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; C081676:thrombin aptamer; D004247:DNA