title: Quantitative PCR analysis of FosB mRNA expression after short duration oxygen and light stress.
abstract: Quantitative polymerase chain reaction (QPCR) was used to examine changes in FosB mRNA expression in models of oxygen and light stress to the retina. C57BL/6 mice or Sprague-Dawley (SD) albino rats were subjected to several experimental paradigms: short-term light or oxygen stress, extended hyperoxia (75% oxygen), or a model of oxygen-induced retinopathy (OIR). Control animals were subjected to room air and 5 lux cyclic light. FosB expression dramatically increases in response to light stress as well as in a model of OIR, but not in response to sustained 75% oxygen. These data suggest that both hypoxia and light stress induce expression of FosB in the retina.
authors.affiliation: School of Optometry, University of California, Berkeley, CA 94720-2020, USA. sgeller@socrates.berkeley.edu
authors.forename: Scott F
authors.initials: SF
authors.lastname: Geller
authors.forename: Jonathan
authors.initials: J
authors.lastname: Stone
mesh_terms: D000818:Animals; D008027:Light; D051379:Mice; D008810:Mice, Inbred C57BL; D010100:Oxygen / Q000008:administration & dosage*; D016133:Polymerase Chain Reaction / Q000379:methods; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D013312:Stress, Physiological / Q000378:metabolism*; D013997:Time Factors; D014157:Transcription Factors
chemical_list: C483819:Fosb protein, mouse; C483820:Fosb protein, rat; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D014157:Transcription Factors; D010100:Oxygen