[Inhibitory effect of hDAF displayed on yeast cells on deposition of complement on the yeast cells].
OBJECTIVE
To investigate whether the hDAF displayed on the surface of yeast cells can inhibit the deposition of C5b-9.


METHODS
After treatment with normal human serum, deposition of human complement C5b-9 and DAF expression on yeast cells EBY100 were analyzed by flow cytometry.


RESULTS
Yeast cells EBY100 could activate the complement molecules in human serum and lead to the deposition of C5b-9, while the deposition of C5b-9 on the surface of yeast cells with hDAF could significantly reduce the deposition of C5b-9.


CONCLUSIONS
hDAF displayed on the surface of yeast cells can fold correctly and exhibit activity of complement's inhibition.
Guo
Bo
B
Department of Immunology, Third Military Medical University, Chongqing 400038, China.
Zheng
Ping
P
Xie
Pei-rong
PR
Wu
Yu-zhang
YZ
Zou
Qiang
Q
D018958:CD55 Antigens / Q000378:metabolism*; D002478:Cells, Cultured; D003167:Complement Activation; D015938:Complement Membrane Attack Complex / Q000378:metabolism*; D006801:Humans; D017510:Protein Folding; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
D018958:CD55 Antigens; D015938:Complement Membrane Attack Complex