In vitro and in vivo inhibitory effects of disodium cromoglycate on influenza virus infection.
Disodium cromoglycate (DSCG) is one of the safest drugs for the prevention of bronchial asthma and allergic rhinitis attacks. The effect of DSCG on acute upper respiratory tract viral infection is still controversial. Here we investigated DSCG inhibition of influenza virus infection in vivo and in vitro. In vivo effects of DSCG on viral infection were assessed using a murine model of respiratory tract infection. Intranasal administration of DSCG protected mice from death induced by infection with influenza virus A/PR/8/34. We analyzed DSCG anti-viral effects in vitro by either (i) treating cells prior to viral adsorption, (ii) treating cells concurrently with viral adsorption, or (iii) treating cells after viral adsorption. DSCG treatment of cells during or after, but not before, viral adsorption significantly inhibited influenza viral infection, indicating DSCG acts on events late in viral infection. DSCG exerts anti-influenza effect both in vitro and in vivo at the doses compatible with treatment for asthma. DSCG marginally inhibited influenza viral neuraminidase and membrane fusion functions, suggesting that DSCG inhibition of viral neuraminidase and fusion activities may partially mediate this anti-influenza effect. Our results indicate that treatment of patients including children with DSCG may take advantages for prevention from influenza virus infection.
Hidari
Kazuya I P J
KI
Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Yada, Shizuoka, Japan. hidari@u-shizuoka-ken.ac.jp
Tsujii
Eisaku
E
Hiroi
Jun
J
Mano
Eriko
E
Miyatake
Akihiko
A
Miyamoto
Daisei
D
Suzuki
Takashi
T
Suzuki
Yasuo
Y
D000281:Administration, Intranasal; D000818:Animals; D002460:Cell Line; D004205:Cromolyn Sodium / Q000008:administration & dosage*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects* / Q000254:growth & development; D007251:Influenza, Human / Q000188:drug therapy* / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C
D004205:Cromolyn Sodium