[Expression of hMSH2 gene in esophageal cancer].
OBJECTIVE
To observe the expression of DNA mismatch repair gene hMSH2 mRNA in esophageal cancer tissues.


METHODS
This study included 32 esophageal cancer patients who received no previous radiotherapy, chemotherapy or other treatments. Within 30 min following surgical removal of the tumor tissues, specimens of the tumor, the tissue adjacent to the tumor and normal tissue at the esophageal stump (1 cmx1 cmx1 cm in size for each specimen) were obtained for examining hMSH2 expression with hMSH2 ISH detection kit.


RESULTS
The positivity rate of hMSH2 was 46.88% in the esophageal cancer tissues, 53.12% in the adjacent tissues, and 84.38% in normal tissues at the esophageal stump, showing significant difference of the former two tissues from the normal tissue (P<0.05). No significant correlation was noted between the positivity rate of hMSH2 and such factors as the patients' age, sex, tumor size, tumor location, pathological type, histological grade, lymphatic metastasis or degree of tumor invasion (P>0.05).


CONCLUSIONS
The deletion of hMSH2 is an early event in the development of esophageal cancer.
Zhang
Gong-yuan
GY
Key Laboratory of Molecular Medicne, Zhengzhou University, Zhengzhou 450052, China. zgy@zzu.edu.cn
Liu
Qiu-liang
QL
Ma
Chun-xiao
CX
Le
Xiao-ping
XP
Ge
Xiu-feng
XF
Ding
Yi
Y
Zhang
Qin-xian
QX
D000368:Aged; D020137:Base Pair Mismatch; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D051718:MutS Homolog 2 Protein; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis
D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; C497172:MSH2 protein, human; D051718:MutS Homolog 2 Protein