title: [Study on the inducible expression of toll-like receptors in human bladder cell line].
abstract: OBJECTIVE
  To investigate and compare the expression of Toll-like receptors (TLRs) in human bladder cells under the stimulus of Escherichia coli (E. coli) versus that under the stimulus of Bacillus Calmette-Guérin (BCG).
  
  
  METHODS
  Human bladder cancer cell line T24 was cultured for 1 hour under the stimuli of various doses (bacillus-cell ratio) of E. coli and BCG respectively. The levels of expression of TLR-2 and TLR-4 mRNA in T24 cells were assessed by RT-PCR method.
  
  
  RESULTS
  By comparison with control, there was no difference observed on the expression of either TLR-2 or TLR-4 in T24 cells under the stimulus of E. coli. The expression level of either TLR-2 or TLR-4 was increased under the stimulus of BCG in a dose-dependent manner. The effects reached the highest level when the dose of BCG was 10 bacilli per cell.
  
  
  CONCLUSIONS
  There exist different expression patterns of TLRs in bladder transitional cells under the different stimuli of E. coli and BCG respectively.
authors.affiliation: Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Gong
authors.forename: Ming
authors.initials: M
authors.lastname: Shi
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wei
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Yu-ru
authors.initials: YR
authors.lastname: Yang
mesh_terms: D001500:BCG Vaccine / Q000276:immunology / Q000494:pharmacology*; D002295:Carcinoma, Transitional Cell / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D004926:Escherichia coli / Q000276:immunology; D006801:Humans; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology
chemical_list: D001500:BCG Vaccine; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; C495342:TLR2 protein, human; C495345:TLR4 protein, human; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors