title: An assessment of different DNA delivery systems for protection against respiratory syncytial virus infection in the murine model: gene-gun delivery induces IgG in the lung.
abstract: Immunization with plasmid DNA (pDNA) has the potential to overcome the difficulties of neonatal vaccination that may be required for protection against infection with respiratory syncytial virus (RSV); however, little is known about optimal delivery modalities. In this pilot study we compared mucosal delivery of pDNA encoding RSV F protein encapsulated in poly(DL-lactide-co-glycolide) with delivery of pDNA by gene-gun for the induction of immunity in mice. Intra-gastric or intra-nasal immunization with various doses of microparticles induced weak low levels of RSV-specific serum antibodies in a proportion of mice; in contrast, gene-gun vaccination led to protective immunity associated with a humoral response. Interestingly, RSV-specific antibody was detected in lung fragment cultures following intradermal vaccination with the gene-gun.
authors.affiliation: Health Protection Agency, Porton Down, Salisbury, Wiltshire SP4 0JG, UK.
authors.forename: Julia A
authors.initials: JA
authors.lastname: Tree
authors.forename: Gary
authors.initials: G
authors.lastname: Bembridge
authors.forename: Sam
authors.initials: S
authors.lastname: Hou
authors.forename: Geraldine
authors.initials: G
authors.lastname: Taylor
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Fashola-Stone
authors.forename: José
authors.initials: J
authors.lastname: Melero
authors.forename: Martin P
authors.initials: MP
authors.lastname: Cranage
mesh_terms: D000818:Animals; D002460:Cell Line; D016503:Drug Delivery Systems / Q000592:standards; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology*; D008171:Lung Diseases / Q000517:prevention & control / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology / Q000517:prevention & control*; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology
chemical_list: D007074:Immunoglobulin G; D019444:Vaccines, DNA; D014764:Viral Proteins