Changes in pit membrane porosity due to deflection and stretching: the role of vestured pits.
The effect of increasing pressure difference (DeltaP) on intervessel pit membrane porosity was studied in two angiosperm tree species with differing pit architecture. Fraxinus americana L. possesses typical angiosperm bordered pit structure while Sophora japonica L. exhibits well-developed vestures in intervessel pit chambers. It was hypothesized (a) that large DeltaP across intervessel pits would cause the deflection of pit membranes in the stems of F. americana resulting in significant increases in porosity and thus lower cavitation thresholds, and (b) that the presence of vestures would prevent the deflection of pit membranes in S. japonica. To determine if the porosity of pit membranes increased under mechanical stress, suspensions of colloidal gold, 5 nm and 20 nm in diameter, were perfused across intervessel pit membranes at DeltaP ranging from 0.25 MPa to 6.0 MPa. The effect of increasing DeltaP on membrane porosity was also tested by comparing air seeding thresholds (Pa) in stems perfused with water or a solution with lower surface tension. Air seeding and colloidal gold experiments indicated that pit membrane porosity increased significantly with DeltaP in F. americana. In S. japonica, increases in permeability to colloidal gold with DeltaP were small and maximum pore diameters predicted from Pa were independent of DeltaP, suggesting that vestures limited the degree to which the membrane can be deflected from the centre of the pit cavity. This provides the first experimental evidence that vestures reduce the probability of air seeding through pit membranes.
Choat
Brendan
B
Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, MA 02138, USA. bchoat@fas.harvard.edu
Jansen
Steven
S
Zwieniecki
Maciej A
MA
Smets
Erik
E
Holbrook
N Michele
NM
D000388:Air; D002463:Cell Membrane Permeability; D003034:Coated Pits, Cell-Membrane / Q000502:physiology* / Q000648:ultrastructure; D020285:Cryoelectron Microscopy; D031661:Fraxinus / Q000502:physiology*; D019684:Magnoliopsida / Q000502:physiology / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D029909:Sophora / Q000502:physiology*; D013314:Stress, Mechanical