title: [Typing for HLA-DR DNA by polymerase chain reaction with sequence-specific primers].
abstract: OBJECTIVE
  To type the HLA-DR DNA for renal transplantation by PCR with sequence specific primers (PCR-SSP).
  
  
  METHODS
  According to nucleotide sequences of HLA-DR, 16 pairs of specific primers and a pair of positive control primers were designed and synthesized for PCR-SSP. Then HLA-DR sites of 52 donors and recipients for renal transplantation were typed by the PCR-SSP.
  
  
  RESULTS
  All the samples were successfully typed by PCR-SSP with the synthesized primers. The results were available within 3 hours after sampling and the accuracy and reproducibility were 100%.
  
  
  CONCLUSIONS
  Genotyping for HLA-DR sites by PCR-SSP with primers reported herein was a simple and accurate technique suitable for clinical application.
authors.affiliation: Department of Urology Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. wuguojun@fmmu.edu.cn
authors.forename: Guo-jun
authors.initials: GJ
authors.lastname: Wu
authors.forename: He
authors.initials: H
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Yu
authors.forename: Jian-lin
authors.initials: JL
authors.lastname: Yuan
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D017931:DNA Primers; D005260:Female; D005838:Genotype; D006684:HLA-DR Antigens / Q000235:genetics*; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D006684:HLA-DR Antigens