Screening specifically expressed ESTs related to piglet diarrhea resistance.
RNA was extracted from spleens of diarrhea and non-diarrhea piglets of Jiuyang Pig, Jianbai Pig and Landrace. RNA pools were established and DDRT-PCR using three anchored and seven arbitrary primers combined with silver staining was conducted to identify ESTs differentially expressed in diarrhea resistance to E. coli K88. Five cDNA were identified in the non-diarrhea RNA pools. Among them two are new sequences and three are highly identical to the ESTs in the GeneBank, of which two have known functions. One is the mammalian Nck adaptor protein 1 and the other is the human LINE-1 reverse transcriptase.
Qi
Xiao-Hong
XH
College of Animal Science and Technology, China Agricultural University, Beijing 100094, China.
Zhang
Qin
Q
Liu
Pei-Qiong
PQ
Song
Chun-Yun
CY
Xu
Ru-Hai
RH
D000818:Animals; D001483:Base Sequence; D003967:Diarrhea / Q000235:genetics / Q000662:veterinary*; D004198:Disease Susceptibility; D020224:Expressed Sequence Tags; D020084:Long Interspersed Nucleotide Elements; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013552:Swine; D013553:Swine Diseases / Q000235:genetics*