[Biocompatibility evaluation of chitosan-g-polyvinylpyrrolidone].
OBJECTIVE
To evaluate the biocompatibility of chitosan-g-polyvinylpyrrolidone as a new scaffold material.


METHODS
The material was tested and measured for water absorption and contact angle, followed by evaluation of the biocompatibility by implantation into rabbits and in vitro cultured with the corneal epithelial cells.


RESULTS
The water absorption rate of the material reached 1 100% with contact angle of 83-86. The results of implantation revealed partial degradation of the material 3 months after implantation, and much collagen and numerous corneal stromal cells appeared on the material without obvious inflammation reactions. In vitro coculture with epithelial cells showed good adhesion of the cells to the material which induced no obvious cytotoxicity.


CONCLUSIONS
The novel chitosan derivative has excellent biocompatibility and can be used as a tissue scaffold material.
Deng
Zheng-xing
ZX
College of Life Science and Technology, Jinan University, Guangzhou 510632, China. deam@tom.com
Zhang
Zhi-xiong
ZX
Li
Li-hua
LH
Zhou
Chang-ren
CR
D000818:Animals; D002478:Cells, Cultured; D002686:Chitin / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000737:chemistry; D048271:Chitosan; D003315:Cornea / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects; D005260:Female; D008297:Male; D008422:Materials Testing; D011205:Povidone / Q000009:adverse effects / Q000737:chemistry; D011817:Rabbits; D023822:Tissue Engineering
D002686:Chitin; D048271:Chitosan; D011205:Povidone