title: Population balance approach to modeling hairy root growth.
abstract: Though numerous models have been developed to describe the growth of microbial cell cultures, far fewer models are available to describe the growth of hairy root cultures. Here a population balance model is proposed to simulate the growth of hairy roots. The model accounts for the increase in biomass due to elongation of a branch by cell division as well as the formation of new branches. The model incorporates the fact that although the likelihood of the formation of a new lateral branch is a maximum at a specific age of the parent branch, lateral branches can form over a distribution of ages of the parent branch. Model parameters are estimated using the genetic algorithm based on experimental data for batch and continuous bioreactors. The model proposed here may provide a better understanding of the increase in biomass of hairy root cultures.
authors.affiliation: Department of Chemical Engineering, Colorado State University, Fort Collins, Colorado 80523-1370, USA.
authors.forename: Binbing
authors.initials: B
authors.lastname: Han
authors.forename: James C
authors.initials: JC
authors.lastname: Linden
authors.forename: Ninad P
authors.initials: NP
authors.lastname: Gujarathi
authors.forename: S Ranil
authors.initials: SR
authors.lastname: Wickramasinghe
mesh_terms: D000465:Algorithms; D018533:Biomass; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D003198:Computer Simulation; D008954:Models, Biological; D018517:Plant Roots / Q000166:cytology* / Q000254:growth & development*; D011157:Population Dynamics