Nanoliter-scale synthesis of arrayed biomaterials and application to human embryonic stem cells.
Identification of biomaterials that support appropriate cellular attachment, proliferation and gene expression patterns is critical for tissue engineering and cell therapy. Here we describe an approach for rapid, nanoliter-scale synthesis of biomaterials and characterization of their interactions with cells. We simultaneously characterize over 1,700 human embryonic stem cell-material interactions and identify a host of unexpected materials effects that offer new levels of control over human embryonic stem cell behavior.
Anderson
Daniel G
DG
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Levenberg
Shulamit
S
Langer
Robert
R
D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology*; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D004622:Embryo, Mammalian / Q000166:cytology*; D006801:Humans; D046228:Microarray Analysis / Q000379:methods*; D011108:Polymers / Q000138:chemical synthesis / Q000494:pharmacology; D013234:Stem Cells / Q000187:drug effects* / Q000378:metabolism
D001672:Biocompatible Materials; D011108:Polymers