title: Quantification of intra- and extra-cellular thermophilic lipase/esterase production by Thermus sp.
abstract: Four Thermus strains produced lipolytic activity when grown in liquid medium for 30 h at 70 degrees C. The highest total lipase/esterase activity (57 U l(-1)) was in Thermus aquaticus YT-1, followed by Thermus thermophilus HB27 and HB8 (33 and 25 U l(-1), respectively), and finally by Thermus sp. (16 U l(-1)). Extra-cellular activity was detected in T. aquaticus YT-1 and T. thermophilus HB27 (33 and 17 U l(-1)). All enzymes were stable at 80 degrees C over 30 min, and their activity towards fatty acid esters increased as substrate chain-length diminished (i.e. hydrolysis rate was up to 6-fold higher on p-nitrophenyl caproate than on laurate).
authors.affiliation: Department of Chemical Engineering, University of Vigo, 36200 Vigo, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Domínguez
authors.forename: A
authors.initials: A
authors.lastname: Sanromán
authors.forename: P
authors.initials: P
authors.lastname: Fuciños
authors.forename: M L
authors.initials: ML
authors.lastname: Rúa
authors.forename: L
authors.initials: L
authors.lastname: Pastrana
authors.forename: M A
authors.initials: MA
authors.lastname: Longo
mesh_terms: D001709:Biotechnology / Q000379:methods; D049109:Cell Proliferation; D004950:Esterases / Q000737:chemistry; D004952:Esters; D005227:Fatty Acids / Q000737:chemistry; D006868:Hydrolysis; D008049:Lipase / Q000737:chemistry; D013379:Substrate Specificity; D013696:Temperature; D013824:Thermus / Q000201:enzymology*; D016963:Thermus thermophilus / Q000201:enzymology; D013997:Time Factors
chemical_list: D004952:Esters; D005227:Fatty Acids; D004950:Esterases; D008049:Lipase