[Experimental study on islet cells in rats under condition of three-dimensional microgravity].
OBJECTIVE
To study the survival rate and secretory function of islet cells in rats under condition of three-dimensional microgravity.


METHODS
Isolated islet cells were assigned to flask-cultured or bioreactor-cultured. Survival rate of islets cultured for days 3, 7, 14 in stationary flasks or microgravity bioreactors was measured by AO-PI double-staining. Cultured islets were identified by dithizone (DTZ) staining, and insulin contents of different culture liquids were measured by radioimmunoassay.


RESULTS
Pancreatic islets stained nacarat with DTZ were easily visualized. When islet cells were cultured for 7 days and 14 days, survival rate of bioreactor-cultured islets was (0.9000 +/- 0.0107)% and 0.8038% +/- 0.0092% and higher than flask-cultured islets (P < 0.01). Insulin level of bioreactor-cultured islets is (70.875 +/- 0.31) m micro /L on the cultured 7 days while flask-cultured islets is (41.246 +/- 0.35) m micro /L. There was statistically significant difference of insulin production between the two groups (P < 0.01). Bioreactor-cultured islet contents were higher than flask-cultured on the cultured 14, 21 and 30 days (P < 0.01).


CONCLUSIONS
Islet cells survival rate and secretory function revealed that bioreactor-cultured islets functioned better compared with flask-cultured islets.
Song
Chun
C
The Key Laboratory of Cell Transplantation of Ministry of Health, Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
Duan
Xiu-qing
XQ
Zhou
Yi
Y
Li
Xi
X
Han
Li-ou
LO
Xu
Ping
P
Song
Chun-fang
CF
Jin
Lian-hong
LH
D000818:Animals; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival; D002478:Cells, Cultured; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018474:Weightlessness Simulation
D007328:Insulin