[Modified nested polymerase chain reaction in one tube for detection of Enterovirus].
A method of a modified nest-type polymerase chain reaction (MN-PCR), made in one tube, was elaborated that enhances the sensitivity and cuts the risk of cross-contamination in enteroviruses (EV) detection. The method, as described in detail above, was used to detect EV RNA in 76.9% and 31.25% of examined autopsy samples (13 liquor and 16 cardiac-tissue samples, respectively). It enabled the detection of EV RNA in 6.25% of samples that used to be negative, when tested by MN-PCR in 2 tubes. MN-PCR with one tube is a reliable, sensitive and specific diagnostic tool; it can be recommended for the routine diagnostics of enterovirus infection.
Poklonskaia
N V
NV
Amvros'eva
T V
TV
D'iakonova
O V
OV
Shcherbakova
O B
OB
Voĭlokova
R Ia
RIa
D004770:Enterovirus / Q000235:genetics* / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000175:diagnosis; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods