title: Magnetically labeled insulin-secreting cells.
abstract: Iron oxide nanoparticles have been shown to magnetically label cells in order to visualize them in vivo via MR imaging. This technology has yet to be implemented in insulin secreting cells, thus it is not known whether the presence of these nanoparticles in the cytoplasm of the cells affects insulin secretion. This study investigates the effectiveness and consequence of labeling mouse insulinoma betaTC3 and betaTC-tet cells with monocrystalline iron oxide nanoparticles (MION). Our data show that MION can be internalized in both betaTC3 and betaTC-tet cells following a 24h exposure to 0.02mg/ml MION solution. The metabolic and secretory activities of both MION-labeled cell lines were statistically indistinguishable from sham treatment. Furthermore, cell viability and apoptosis remained constant throughout the cell's exposure to MION. Finally, MR images demonstrated significant contrast between labeled and sham-treated cells. Thus, labeling murine insulinoma cell lines with magnetic iron oxide nanoparticles does not hinder their insulin secretion, while it provides MR imaging contrast.
authors.affiliation: Division of Endocrinology, Department of Medicine, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Jose
authors.initials: J
authors.lastname: Oca-Cossio
authors.forename: Hui
authors.initials: H
authors.lastname: Mao
authors.forename: Nata
authors.initials: N
authors.lastname: Khokhlova
authors.forename: Christopher McE
authors.initials: CM
authors.lastname: Kennedy
authors.forename: Jonathan W
authors.initials: JW
authors.lastname: Kennedy
authors.forename: Cheryl L
authors.initials: CL
authors.lastname: Stabler
authors.forename: Encai
authors.initials: E
authors.lastname: Hao
authors.forename: Athanassios
authors.initials: A
authors.lastname: Sambanis
authors.forename: Nicholas E
authors.initials: NE
authors.lastname: Simpson
authors.forename: Ioannis
authors.initials: I
authors.lastname: Constantinidis
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D008280:Magnetics; D051379:Mice
chemical_list: D007328:Insulin