Binding of the new morphiceptin analogs to human MCF-7 breast cancer cells and their effect on growth.
In the present study, we reported on the synthesis of two new mu-opioid peptide analogs, [D-1-Nal3]morphiceptin and [D-1-Nal4]-morphiceptin [1-Nal=3-(1-naphthyl)-alanine] which expressed receptor binding affinities at least at the level of the primary opioid ligands. The new analogs also labeled mu-opioid receptors on the cells of human breast cancer MCF-7 cell line with affinity much higher than that of endomorphins and morphiceptin, the well-known mu-selective opioid peptides. However, none of the tested peptides significantly decreased cell proliferation of MCF-7 cells.
Janecka
Anna
A
Department of Medicinal Chemistry, Medical University, Lodz, Mazowiecka 6/8, 92-215, Poland. ajanecka@zdn.am.lodz.pl
Fichna
Jakub
J
Kosson
Piotr
P
Zalewska-Kaszubska
Jadwiga
J
Krajewska
Urszula
U
Mirowski
Marek
M
Rozalski
Marek
M
D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects*; D003292:Convulsants / Q000378:metabolism*; D004723:Endorphins / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D017450:Receptors, Opioid, mu / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D003292:Convulsants; D004723:Endorphins; D008024:Ligands; D009842:Oligopeptides; D017450:Receptors, Opioid, mu; C105293:endomorphin 1; C105294:endomorphin 2; C028889:morphiceptin