Interferences in clinical capillary zone electrophoresis of serum proteins.
Capillary zone electrophoresis (CZE) of serum proteins has become a well-accepted method for the separation of serum proteins and for the detection of monoclonal proteins in clinical laboratories. As CZE uses ultraviolet detection for direct protein quantification, exogenous ultraviolet-absorbing substances are a novel challenge, not present in conventional gel-based methods in which protein is quantified by dye binding. This minireview gives a survey of interfering substances, including iodinated contrast agents and antibiotics.
Bossuyt
Xavier
X
Laboratory Medicine/Immunology, University Hospitals Leuven, Herestraat 49, B-3000 Leuven, Belgium. xavier.bossuyt@uz.kuleuven.ac.be
D000042:Absorption; D000900:Anti-Bacterial Agents / Q000737:chemistry; D000890:Anti-Infective Agents / Q000737:chemistry*; D001797:Blood Protein Electrophoresis / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis*; D003287:Contrast Media / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D006801:Humans; D007482:Iothalamate Meglumine / Q000737:chemistry; D013420:Sulfamethoxazole / Q000737:chemistry
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D001798:Blood Proteins; D003287:Contrast Media; C001694:meglumine ioxithalamate; D013420:Sulfamethoxazole; D007482:Iothalamate Meglumine