title: Hypoxia inhibits the expression of the CCR5 chemokine receptor in macrophages.
abstract: Hypoxia, a decrease in oxygen tension occurring in pathological tissues, has a profound effect on macrophage functions. Here, we provide the first evidence that hypoxia inhibits CCR5 chemokine receptor expression in mouse macrophages. CCR5 was constitutively expressed in macrophages and upregulated by IFNgamma. Hypoxia downregulated both constitutive and IFNgamma-induced CCR5 mRNA and protein. Reoxygenation of hypoxic cells reverted CCR5 inhibition. CCR5 upregulation by IL-10, LPS, and IL-4 was also antagonized by hypoxia. CCR5 inhibition may be a way to retain/concentrate recruited macrophages at hypoxic sites or a feedback mechanism to control the autocrine activation of macrophages which produce CCR5 ligands.
authors.affiliation: Laboratory of Molecular Biology, G. Gaslini Institute, 16147 Genova, Italy. mcbosco1@virgilio.it
authors.forename: Maria Carla
authors.initials: MC
authors.lastname: Bosco
authors.forename: Gioia
authors.initials: G
authors.lastname: Reffo
authors.forename: Maura
authors.initials: M
authors.lastname: Puppo
authors.forename: Luigi
authors.initials: L
authors.lastname: Varesio
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D002460:Cell Line; D015536:Down-Regulation; D005786:Gene Expression Regulation; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism; D019713:Receptors, CCR5 / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D019713:Receptors, CCR5