In vivo protein markers of human peritoneal mesothelial cells: do they differ according to their anatomical sites?
OBJECTIVE
To highlight the in vivo protein markers in human peritoneal mesothelial cells according to their anatomical distribution and their interest in theories of peritoneal repair.


METHODS
Clinical laboratory study.


METHODS
University Hospital, France.


METHODS
Sampling of mesothelial cells during 8 laparotomies by impression smears.


METHODS
Immunochemical semiquantitative measures of proteins.


RESULTS
There were uniform ratios of protein markers among the whole peritoneal mesothelial cell population, whatever their anatomical origin (liver, stomach, omentum, small bowel, and abdominal wall).


CONCLUSIONS
This is consistent with the surrounding uninjured mesothelial cells or the exfoliated mesothelial cells, or both, having a role in postoperative peritoneal mesothelial repair.
Serre
Valérie
V
Laboratoire Cytosquelette et Développement, CNRS UMR 7000, Faculté de Médecine Pitié-Salpêtrière, Paris, France.
Tézenas du Montcel
Sophie
S
Kontula
Linda
L
Simon
Monique
M
Perret
Emmanuelle
E
Nédellec
Patrick
P
Vicart
Patrick
P
Duron
Jean-Jacques
JJ
D000368:Aged; D015415:Biomarkers / Q000032:analysis; D004847:Epithelial Cells / Q000737:chemistry* / Q000166:cytology; D005455:Fluorescent Antibody Technique; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis*; D018613:Microscopy, Confocal; D008875:Middle Aged; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000032:analysis*; D010537:Peritoneum / Q000737:chemistry / Q000166:cytology*; D014781:Viscera / Q000737:chemistry / Q000166:cytology*; D038203:alpha-Crystallin B Chain / Q000032:analysis*
D015415:Biomarkers; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; D038203:alpha-Crystallin B Chain; D007633:Keratins