[A study on the effects of cells and scaffolds tissue engineering on the periodontal regeneration].
OBJECTIVE
To observe the effects of cells and scaffolds tissue engineering on the periodontal regeneration, and to evaluate the feasibility of nano-Hap-collagen (nHAC) as the scaffold material for periodontal tissue engineering.


METHODS
Dog autogenous periodontal ligament cells (PDLCs) cultured in vitro were collected and seeded on the three-dimensional framework of nHAC. The cell growth in the scaffolds was observed by scanning electron microscope. And then the PDLCs-nHAC composites were transplanted into man-made periodontal defects, and the groups filled with nothing or filled only with nHAC were the controls. The dogs were sacrificed after 8 weeks and the periodontal regeneration was observed histologically.


RESULTS
Scanning electron microscope showed the porous structure of nHAC and the eugonic growth of cells in the nHAC scaffolds. The histological observation showed that the PDLCs-nHAC groups exhibited more new bone, new periodontal ligament and new cementum occupying the majority of the defects than the control groups, and the epitheliums were not observed.


CONCLUSIONS
Periodontal regeneration could be enhanced by the cells and scaffolds tissue engineering, and the PDLCs and nHAC could be used as the seed cell and the scaffold material for periodontal tissue engineering.
Lu
Hong
H
Department of Periodontology, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Wu
Zhi-Fen
ZF
Tian
Yu
Y
D000818:Animals; D001672:Biocompatible Materials; D002478:Cells, Cultured; D004285:Dogs; D048091:Guided Tissue Regeneration; D008297:Male; D017622:Periodontal Attachment Loss / Q000601:surgery*; D010513:Periodontal Ligament / Q000166:cytology*; D012038:Regeneration; D012092:Replantation / Q000379:methods; D023822:Tissue Engineering / Q000379:methods*; D014162:Transfection
D001672:Biocompatible Materials