Subcellular distribution of alpha1 and beta2/3 GABA(A) receptor subunits in sensory neurons of the bovine trigeminal mesencephalic nucleus: evidence suggesting their axoplasmic transport.
Neurons were free-hand isolated under the stereomicroscope from the bovine trigeminal mesencephalic nucleus. These neurons were challenged with monoclonal antibodies against the alpha1 and the beta2/3 subunits of the GABA(A) receptor. The neurons showed a strong reaction to both antibodies. The reaction was mainly intracellular in the round cell bodies and in the axoplasm of the axon emerging from these pseudounipolar cells. This suggests the synthesis of these subunits in the cell body and their transport along the axon.
Cupello
Aroldo
A
Istituto di Bioimmagini e Fisiologia Molecolare, CNR, Sezione di Genova, Genova, Italy. dcupel@neurologia.unige.it
Luccardini
Camilla
C
Rapallino
Maria Vittoria
MV
Robello
Mauro
M
Seitun
Andrea
A
D000818:Animals; D001370:Axonal Transport; D002417:Cattle; D008636:Mesencephalon / Q000502:physiology*; D009475:Neurons, Afferent / Q000502:physiology*; D021122:Protein Subunits / Q000378:metabolism; D011963:Receptors, GABA-A / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
D021122:Protein Subunits; D011963:Receptors, GABA-A