title: The effects of long-term diet and omega-3 fatty acid supplementation on coagulation factor VII and serum phospholipids with special emphasis on the R353Q polymorphism of the FVII gene.
abstract: The aim of the present study was to investigate the effect of long-term diet and very long chain n-3 fatty acids (VLC n-3) intervention on plasma coagulation factor VII (FVII), choline-containing phospholipids (PC) and triglycerides (TG), especially related to the R353Q polymorphism of the FVII gene. The present investigation included 219 subjects from the Diet and Omega-3 Intervention Trial on atherosclerosis (DOIT), a 2x2 factorial designed study in elderly men with long-standing hypercholesterolemia. The subjects were randomly allocated to receive placebo capsules (corn oil) (control), placebo capsules and dietary advice ("Mediterranean type" diet), VLC n-3 capsules, or VLC n-3 capsules and dietary advice combined. The R353Q genotype and the levels of FVIIc, FVIIag, FVIIa, PC, and TG at baseline and after 6 months were determined. Diet intervention was followed by a significant reduction of 5.1% in the levels of FVIIag and 2.4 mU/ml in FVIIa (95% CI -7.4, -2.9, and -3.8, -1.1, respectively) (both p<0.001) compared to the no diet group, independent of genotype. No effects of diet intervention on FVIIc, PC or TG were observed. After VLC n-3 supplementation the TG levels were significantly reduced compared to placebo (p=0.01), whereas all FVII levels and PC remained unchanged. Dietary advice towards a "Mediterranean type" diet, but not VLC n-3 supplementation, was shown to reduce the levels of FVIIag and FVIIa after 6 months, independent of genotype. The results indicate the dietary advice to be more favourable in reducing this risk factor for CVD as compared to specific VLC n-3 supplementation.
authors.affiliation: The University College of Akershus, Lillestrøm, Norway. anjal@basalmed.uio.no
authors.forename: Anja S
authors.initials: AS
authors.lastname: Lindman
authors.forename: Jan I
authors.initials: JI
authors.lastname: Pedersen
authors.forename: Elsa M
authors.initials: EM
authors.lastname: Hjerkinn
authors.forename: Harald
authors.initials: H
authors.lastname: Arnesen
authors.forename: Marit B
authors.initials: MB
authors.lastname: Veierød
authors.forename: Ingrid
authors.initials: I
authors.lastname: Ellingsen
authors.forename: Ingebjørg
authors.initials: I
authors.lastname: Seljeflot
mesh_terms: D000368:Aged; D001161:Arteriosclerosis / Q000188:drug therapy; D003327:Coronary Disease / Q000517:prevention & control; D019587:Dietary Supplements; D005167:Factor VII / Q000032:analysis / Q000187:drug effects* / Q000235:genetics*; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D008297:Male; D020125:Mutation, Missense; D010713:Phosphatidylcholines / Q000097:blood; D010743:Phospholipids / Q000097:blood*; D020641:Polymorphism, Single Nucleotide; D012306:Risk; D014280:Triglycerides / Q000097:blood
chemical_list: D015525:Fatty Acids, Omega-3; D010713:Phosphatidylcholines; D010743:Phospholipids; D014280:Triglycerides; D005167:Factor VII