title: Agonistic effect of imidazole and triazole fungicides on in vitro oocyte maturation in rainbow trout (Oncorhynchus mykiss).
abstract: The purpose of the present study was to assess the in vitro effect of some imidazole (prochloraz, imazalil) and triazole (epoxiconazole) agricultural fungicides on gonadotropin-induced oocyte maturation in rainbow trout. Results show that prochloraz, epoxiconazole and imazalil strongly potentiated the induction of oocyte maturation by gonadotropin in a dose-dependent manner. Furthermore, 10(-5) M prochloraz and epoxiconazole alone induced oocyte maturation. The mRNA biosynthesis inhibitor, actinomycin d, completely inhibited oocyte maturation induced by fungicides, suggesting that the gonadotropin-like effect of these chemicals is at least dependent on de novo gene expression.
authors.affiliation: INRA, SCRIBE, Campus de Beaulieu, 35042 Rennes cedex, France. monod@beaulieu.rennes.inra.fr
authors.forename: Gilles
authors.initials: G
authors.lastname: Monod
authors.forename: Hélène
authors.initials: H
authors.lastname: Rime
authors.forename: Julien
authors.initials: J
authors.lastname: Bobe
authors.forename: Bernard
authors.initials: B
authors.lastname: Jalabert
mesh_terms: D000818:Animals; D003609:Dactinomycin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005659:Fungicides, Industrial / Q000037:antagonists & inhibitors / Q000633:toxicity*; D007093:Imidazoles / Q000037:antagonists & inhibitors / Q000633:toxicity*; D066298:In Vitro Techniques; D017686:Oncorhynchus mykiss / Q000254:growth & development / Q000378:metabolism*; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012741:Sexual Maturation / Q000187:drug effects*; D014230:Triazoles / Q000037:antagonists & inhibitors / Q000633:toxicity*
chemical_list: D005659:Fungicides, Industrial; D007093:Imidazoles; D011500:Protein Synthesis Inhibitors; D014230:Triazoles; D003609:Dactinomycin