title: Comparison of antioxidant activity and bioavailability of tea epicatechins with their epimers.
abstract: Canned and bottled tea drinks contain not only green tea epicatechins (GTE), namely (-)-epigallocatechin gallate (EGCG), (-)-epicatechin gallate (ECG), (-)-epigallocatechin (EGC) and (-)-epicatechin (EC), but also four GTE epimers, namely (-)-gallocatechin gallate (GCG), (-)-catechin gallate (CG), (-)-gallocatechin (GC) and (-)-catechin (C). In the present study we examined the antioxidant activity and bioavailability of these epimers compared with their corresponding precursors. The epimerisation reaction was induced by autoclaving GTE extract derived from longjing green tea at 120 degrees C for 20 min. Isolation and purification of each GTE and epimer were accomplished by various column chromatographic and semi-preparative HPLC techniques. The antioxidant activity of each epimer with its corresponding GTE precursor was conducted in the three in vitro systems, namely human LDL oxidation, ferric reducing-antioxidant power (FRAP), and anti-2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical assays. The results of all three assays demonstrated that CG had similar antioxidant activity with its precursor ECG, while GC was less potent as an antioxidant than its precursor EGC. Regarding EGCG and GCG, the antioxidant potency was similar for both LDL oxidation and DPPH free radical assays, but GCG was statistically less effective than EGCG in the FRAP assay. For EC and C, the latter had less anti-free radical activity in the DPPH assay, but in LDL oxidation and FRAP assays the antioxidant activity was similar. Oral and intravenous dosing of GTE-epimer mixture led to increase in total plasma antioxidant capacity in rats. In general, both epicatechins and epimers had low bioavailability (0.08-0.31) and most of the observed differences between epicatechins and their corresponding epimers were small, even if they were statistically significant in some cases. It was concluded that the epimerisation reaction occurring in manufacturing canned and bottled tea drinks would not significantly affect antioxidant activity and bioavailability of total tea polyphenols.
authors.affiliation: Department of Biochemistry, The Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Jin Ze
authors.initials: JZ
authors.lastname: Xu
authors.forename: Sai Ying Venus
authors.initials: SY
authors.lastname: Yeung
authors.forename: Qi
authors.initials: Q
authors.lastname: Chang
authors.forename: Yu
authors.initials: Y
authors.lastname: Huang
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Chen
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000493:pharmacokinetics / Q000494:pharmacology*; D001682:Biological Availability; D001713:Biphenyl Compounds / Q000737:chemistry; D002392:Catechin / Q000493:pharmacokinetics / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000378:metabolism; D006801:Humans; D006834:Hydrazines / Q000737:chemistry; D007501:Iron / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D008657:Metabolic Clearance Rate; D010851:Picrates; D051381:Rats; D017207:Rats, Sprague-Dawley; D013662:Tea / Q000737:chemistry*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D006834:Hydrazines; D008077:Lipoproteins, LDL; D010851:Picrates; D013662:Tea; D017392:Thiobarbituric Acid Reactive Substances; D002392:Catechin; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron