[Efficiency of lymphocytes adhesion to endothelial cells of distinct tissue origin from children with asthma].
Lymphocytes extravasation is determined by their adhesion to the endothelial cells. The process goes along with high degree selectivity and is limited to lymph nodes and lymphatic tissue. It comes to uncontrolled extravasation to the tissues of involved organs in diseases with allergic origin as well in inflammation. Most information concerning adhesive interactions comes from researches dealing with normal lymphocytes. However, this phenomenon plays also crucial role in inflammation, metastasis and other pathologies. The aim of the work was to search for the possible distinct efficacy of adhesive interactions between peripheral blood lymphocytes from children with asthma and endothelial cells isolated from lung, skin and intestine. Isolated peripheral blood leukocytes were overlayered into endothelial cell monolayer. After washing the unbound cells, adhering lymphocytes were collected with endothelial cells. For quantifying the percentage of the particular cell populations cytofluorometric method was applied. The results are presented as a number of adhering lymphocytes per one endothelial cell. It has been shown that lymphocytes from asthmatic children have significantly greater adhesive potential towards endothelial cell lines from lungs and skin as compared with adhesion to endothelium of intestine origin. However, their B lymphocytes subpopulation demonstrated significantly higher percentage of cells adhering to all endothelial cell lines tested, as compared with B lymphocytes from normal controls. There were not statistically different adhesion efficiencies of T lymphocytes and NK cells. These findings indicate that local, tissue specific adhesive leukocyte-endothelial interactions may be of some importance in pathogenesis of allergic diseases.
Lewandowicz-Uszyńska
Aleksandra
A
Katedra Propedeutyki Pediatrii Kliniki Immunologii Wieku Rozwojowego AM we Wrocławiu. alusz@dilnet.wroc.pl
Paprocka
Maria
M
Kieda
Claudine
C
Duś
Danuta
D
D000293:Adolescent; D000328:Adult; D001249:Asthma / Q000378:metabolism / Q000503:physiopathology*; D002448:Cell Adhesion; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D004727:Endothelium / Q000378:metabolism*; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male