Characterization of canine caspase-3.
The canine caspase-3 gene was cloned and sequenced. The canine caspase-3 cDNA clone was 1473 base pairs in length and encoded 277 amino acids. The predicted amino acid sequence showed 88.4%, 88.0%, 85.9%, 65.9% and 60.6% homology with that of human, pig, mouse, chicken and zebra fish caspase-3, respectively. The caspase-3 mRNA was confirmed to express in skin, lymph node, bone marrow, small intestine and lung from a healthy dog by RT-PCR analysis.
Sano
Junichi
J
Department of Pathobiology, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, Japan.
Oguma
Keisuke
K
Kano
Rui
R
Hasegawa
Atsuhiko
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D053148:Caspase 3; D020169:Caspases / Q000235:genetics*; D016000:Cluster Analysis; D017931:DNA Primers; D004285:Dogs / Q000235:genetics*; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000378:metabolism; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D012333:RNA, Messenger; C505407:CASP3 protein, human; C505409:Casp3 protein, mouse; D053148:Caspase 3; D020169:Caspases