Enhancement of photosynthetic O2 evolution in Chlorella vulgaris under high light and increased CO2 concentration as a sign of acclimation to phosphate deficiency.
The photosynthetic oxygen evolution of Chlorella vulgaris (Beijer.) cells taken from phosphate-deficient (-P) and control cultures was measured during 8 days of culture growth. Under inorganic carbon concentration (50 microM) in the measuring cell suspension and irradiance (150 micromol m(-2) s(-1)), the same as during culture growth, there were no marked differences in the photosynthetic O2 evolution rate between the -P cells and the controls. The much slower growth of -P cultures indicated that the utilization of absorbed photosynthetically active radiation (PAR) in the CO2 assimilation and biomass production were in -P cells less efficient than in the controls. Alga cells under the phosphorus stress utilized more of the absorbed PAR in the nitrate reduction than the control cells. However, under conditions of more efficient CO2 supply (inorganic carbon concentration 150 microM, introducing of exogenous carbonic anhydrase to the measuring cell suspension) and under increased irradiance (500 micromol m(-2) s(-1)), the photosynthetic O2 evolution in -P cells reached a higher rate than in the controls. The results suggest that in -P cells the restricted CO2 availability limits the total photosynthetic process. But under conditions more favorable for the CO2 uptake and under high irradiance, the -P cells may reveal a higher photosynthetic oxygen evolution rate than the controls. It is concluded that an increased potential activity of the photosynthetic light energy absorption and conversion in the C. vulgaris cells from -P cultures is a sign of acclimation to phosphorus stress by a sun-type like adaptation response of the photosynthetic apparatus.
Kozłowska-Szerenos
Bozena
B
Institute of Biology, The University of Białystok, Swierkowa 20B, 15-950 Białystok, Poland. bokoz@uwb.edu.pl
Bialuk
Izabela
I
Maleszewski
Stanisław
S
D002256:Carbonic Anhydrases / Q000737:chemistry / Q000378:metabolism; D002708:Chlorella / Q000378:metabolism*; D007700:Kinetics; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000737:chemistry / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism; D010788:Photosynthesis; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
D009566:Nitrates; D010710:Phosphates; D000644:Quaternary Ammonium Compounds; D010758:Phosphorus; D009565:Nitrate Reductases; D050901:Nitrate Reductase; D002256:Carbonic Anhydrases; D010100:Oxygen