title: Fly ash leachate induces oxidative stress in freshwater fish Channa punctata (Bloch).
abstract: Oxidative stress inducing potential of fly ash leachate (FAL) was studied in a freshwater fish, Channa punctata (Bloch). Fish were exposed to fly ash leachate for 24 h and lipid peroxidation (LPO) was studied as a marker of oxidative stress. Catalase (CAT), glutathione S-transferase (GST) activities and levels of reduced glutathione (GSH) were also estimated in the exposed fish. FAL (1 ml/l) induced LPO in all the organs and most prominent response was in the gill. It also caused induction of enzymes and glutathione. Liver showed highest level of induction of enzyme activities. The results of this study demonstrate that fly ash constituents have potential to induce oxidative stress in fish and gills are the most vulnerable organs. It is also suggested that in case of exposure to FAL, along with LPO antioxidant defense is also activated to counteract the reactive oxygen species (ROS) at least partly in the initial stages of exposure.
authors.affiliation: Department of Medical Elementology and Toxicology, Ecotoxicology Laboratory, Jamia Hamdard (Hamdard University), New Delhi 110062, India.
authors.forename: M
authors.initials: M
authors.lastname: Ali
authors.forename: S
authors.initials: S
authors.lastname: Parvez
authors.forename: S
authors.initials: S
authors.lastname: Pandey
authors.forename: F
authors.initials: F
authors.lastname: Atif
authors.forename: M
authors.initials: M
authors.lastname: Kaur
authors.forename: H
authors.initials: H
authors.lastname: Rehman
authors.forename: S
authors.initials: S
authors.lastname: Raisuddin
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002244:Carbon / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D060729:Coal Ash; D004790:Enzyme Induction / Q000187:drug effects; D005618:Fresh Water; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007194:India; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D052638:Particulate Matter; D010473:Perciformes / Q000502:physiology*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D060729:Coal Ash; D052638:Particulate Matter; D014874:Water Pollutants, Chemical; D002244:Carbon; D002374:Catalase; D005982:Glutathione Transferase; D005978:Glutathione