[Usefulness of the E-test and its assay conditions in the study of the interaction of antifungal agents. A pilot study].
Preliminary data from a pilot study to assess the usefulness of the E-test in the study of antimycoctics are presented, evaluating assay and reproduction conditions. Results are compared with those obtained using the checkerboard method and mortality-time curves. Trials were carried out with a strain of Candida albicans (ATCC 90028). The E-test strips were combined in direct proportion, MIC-MIC, and in inverse proportion. The results showed that the method can be reproduced, is easy to carry out and may be suitable for the study of the in vitro interaction of antimycotics on yeast. The directly and inversely proportionate strip combination appeared to be the most suitable. At the prediffusion stage, the most highly water-soluble antimycotic should be used. The recommended time for prediffusion is one hour for water-soluble antimycotics, and two hours for non-water-soluble ones. The E-test showed good correlation with mortality-time curves. Nonetheless, in vivo correlation studies are required to determine the usefulness of the results in vitro and the most suitable method of measurement
Cantón
E
E
Unidad de Microbiologia Experimental, Centro de Investigacion y Servicio de Microbiologia, Hospital Universitario La Fe, Valencia, Spain. canton_emi@gva.es
Pemán
J
J
Romero
M
M
Gobernado
M
M
D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D001393:Azoles / Q000494:pharmacology*; D004347:Drug Interactions; D005437:Flucytosine / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009281:Naphthalenes / Q000494:pharmacology*; D010865:Pilot Projects; D015203:Reproducibility of Results; D000077291:Terbinafine
D000935:Antifungal Agents; D001393:Azoles; D009281:Naphthalenes; D000666:Amphotericin B; D005437:Flucytosine; D000077291:Terbinafine