Quantitative analysis of free ubiquitin and multi-ubiquitin chain in colorectal cancer.
Accumulation of ubiquitin (Ub) has been documented in various cancers. The levels of the two forms of Ub, i.e. free ubiquitin (FUb) and multi-ubiquitin chain (MUC) were measured in colorectal cancers and in matched normal colonic tissues in 43 patients. When compared to normal colonic tissues, the levels of both FUb and MUC were significantly higher in the cancerous tissues (P < 0.01, respectively). The level of FUb was related to the depth of invasion (P < 0.05). The level of MUC was related to blood vessel invasion and lymphatic vessel invasion (P < 0.05). The levels of FUb and MUC had no correlation with histological grade, lymph node metastasis, liver metastasis or relapse-free survival. We conclude that the levels of FUb and MUC were up-regulated in colorectal cancer and were correlated with the pathological findings.
Ishibashi
Yoshio
Y
Department of Surgery, Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan. ishibashi@jikei.ac.jp
Hanyu
Nobuyoshi
N
Suzuki
Yutaka
Y
Yanai
Shinichiro
S
Tashiro
Kenichi
K
Usuba
Teruyuki
T
Iwabuchi
Shuichi
S
Takahashi
Tomoko
T
Takada
Koji
K
Ohkawa
Kiyoshi
K
Urashima
Mitsuyoshi
M
Yanaga
Katsuhiko
K
D000328:Adult; D000368:Aged; D003106:Colon / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011863:Radioimmunoassay; D025801:Ubiquitin / Q000378:metabolism*
D025801:Ubiquitin