Identification of unique transcripts from a mouse full-length, subtracted inner ear cDNA library.
A small-scale full-length library construction approach was developed to facilitate production of a mouse full-length cDNA encyclopedia representing approximately 250 enriched, normalized, and/or subtracted cDNA libraries. One library produced using this approach was a subtracted adult mouse inner ear cDNA library (sIEa). The average size of the inserts was approximately 2.5 kb, with the majority ranging from 0.5 to 7.0 kb. From this library 22,574 sequence reads were obtained from 15,958 independent clones. Sequencing and chromosomal localization established 5240 clusters, with 1302 clusters being unique and 359 representing new ESTs. Our sIEa library contributed 56.1% of the 7773 nonredundant Unigene clusters associated with the four mouse inner ear libraries in the NCBI dbEST. Based on homologous chromosomal regions between human and mouse, we identified 1018 UniGene clusters associated with the deafness locus critical regions. Of these, 59 clusters were found only in our sIEa library and represented approximately 50% of the identified critical regions.
Beisel
Kirk W
KW
Department of Biomedical Sciences, Creighton University, 2500 California, Omaha, NE 68178, USA. beisel@creighton.edu
Shiraki
Toshiyuki
T
Morris
Ken A
KA
Pompeia
Celine
C
Kachar
Bechara
B
Arakawa
Takahiro
T
Bono
Hidemasa
H
Kawai
Jun
J
Hayashizaki
Yoshihide
Y
Carninci
Piero
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003638:Deafness / Q000235:genetics*; D007758:Ear, Inner / Q000378:metabolism*; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D015723:Gene Library; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic