Flow-cytometric DNA analysis of intracranial tumors in children.
The objective of this study was to investigate flow-cytometric DNA values of pediatric intracranial tumors, and to establish DNA analysis as a potential prognostic parameter. Twenty-nine brain tumor specimens from 26 pediatric patients were cryo-preserved within a 3-year period. The DNA content was measured by flow cytometry. Six of the tumor specimens had aneuploid DNA patterns. The median of the proliferation index was lower in the survivor group compared with the non-survivor group (36.4% and 47.5%, respectively). Ten of the 26 patients are still alive, eight were lost to follow up, and eight died. Flow-cytometric DNA analysis may be a helpful tool for examining brain tumors in children. The small size of this study could not establish flow cytometry as a definite prognostic factor, but further prospective multicenter studies will evaluate the prognostic significance of flow-cytometric DNA analysis.
Habermehl
P
P
Pediatric Hospital and Ambulant Clinic of Johannes Gutenberg-University Mainz, Germany. pirmin.habermehl@uni-mainz.de
Knuf
M
M
Schwarz
M
M
Bohl
J
J
Bartels
U
U
Gutjahr
P
P
Hohenfellner
K
K
D000293:Adolescent; D000782:Aneuploidy; D001932:Brain Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D049109:Cell Proliferation; D002648:Child; D002675:Child, Preschool; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007223:Infant; D008297:Male; D011379:Prognosis; D015996:Survival Rate
D004273:DNA, Neoplasm