title: Structural elucidation of the extracellular and cell-wall teichoic acids of Staphylococcus epidermidis RP62A, a reference biofilm-positive strain.
abstract: The ability to adhere to artificial surfaces and form biofilms is considered as a virulence factor of Staphylococcus epidermidis, one of the major causes of nocosomial infections, especially those related to implanted medical devices. Cell-wall teichoic acid is known to play an important role in biofilm formation of staphylococci. The structure of the cell wall and extracellular teichoic acids of S. epidermidis RP62A, a reference biofilm-positive strain, was studied by NMR spectroscopy and capillary electrophoresis-mass spectrometry. Their structures were found to be a (1-->3)-linked poly(glycerol phosphate), substituted at the 2-position of glycerol residues with alpha-Glc, alpha-GlcNAc, D-Ala and alpha-Glc6Ala. D-Alanyl acylation of a sugar hydroxyl group seems to be a novel structural feature of teichoic acids from staphylococci.
authors.affiliation: Laboratoire de Recherche sur les Biomatériaux et les Biotechnologies, Université du Littoral-Côte d'Opale, INSERM IR2 Bassin Napoléon BP 120, F-62327 Boulogne-sur-mer, France.
authors.forename: Irina
authors.initials: I
authors.lastname: Sadovskaya
authors.forename: Evgueny
authors.initials: E
authors.lastname: Vinogradov
authors.forename: Jianjun
authors.initials: J
authors.lastname: Li
authors.forename: Saïd
authors.initials: S
authors.lastname: Jabbouri
mesh_terms: D018441:Biofilms; D002473:Cell Wall / Q000737:chemistry*; D005110:Extracellular Space / Q000737:chemistry*; D015394:Molecular Structure; D013212:Staphylococcus epidermidis / Q000737:chemistry*; D013682:Teichoic Acids / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D013682:Teichoic Acids