Association between CD62 ligand on naïve and memory T cells and history of cow's milk hypersensitivity in atopic patients.
BACKGROUND
Both IgE and non-IgE cell-mediated reactions can contribute to the immunopathogenesis of food hypersensitivity.


OBJECTIVE
To study L-selectin expression in naïve (CD45RA+) and memory (CD45RO+) T cells in atopic patients with a history of cow's milk allergy and CD69 expression.


METHODS
We evaluated 12 children with a history of cow's milk allergy. All 12 children underwent clinical history, examination, in vivo skin prick testing to inhalants and milk allergen, and in vitro radioallergosorbent testing for milk and total serum IgE levels. L-selectin levels on naive and memory T cells from patients and controls were analyzed by flow cytometry with fluorochrome-conjugated monoclonal antibodies after 4 hours or 7 days of incubation with medium alone, 1 microg/mL of pokeweed mitogen, or 50 microg/mL of casein.


RESULTS
The percentage of CD45RA+ 62L+ cells with casein was 56% +/- 11% vs 38% +/- 7.7% in healthy controls (P < .009). The percentage of CD45RO- 62L+ cells was 22% +/- 7.1% vs 39% +/- 7.6% in healthy controls (P < .001), whereas the expression of CD62L+ in CD45RA+ and CD45RO+ cells decreased 149% +/- 33% vs 540% +/- 36% for healthy controls (P < .001) and 89% -+/- 16% vs 402% +/- 103% for healthy controls (P < .001), respectively.


CONCLUSIONS
The results of the present study demonstrate the presence of CD62 ligand on naive and memory T cells, which might serve as a predictive marker for atopic dermatitis withother overlapping atopic disorders, such as asthma and allergic rhinitis.
Frieri
Marianne
M
Division of Allergy Immunology and Clinical Immunopathology, Nassau University Medical Center, East Meadow, New York 11554, USA. mfrieri@numc.edu
Bennuri
Bindu
B
Dias
Celso
C
Huang
Yu-Ching
YC
Schaefer
Peter
P
D000293:Adolescent; D015703:Antigens, CD / Q000096:biosynthesis / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis / Q000276:immunology; D015415:Biomarkers; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood; D007223:Infant; D019041:L-Selectin / Q000096:biosynthesis* / Q000276:immunology; D037181:Lectins, C-Type; D017493:Leukocyte Common Antigens / Q000276:immunology; D008297:Male; D016269:Milk Hypersensitivity / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D015415:Biomarkers; D007073:Immunoglobulin E; D019041:L-Selectin; D037181:Lectins, C-Type; D017493:Leukocyte Common Antigens; D000099192:CD69 Antigens