title: Electrochemical genosensor for mitomycin C-DNA interaction based on guanine signal.
abstract: The interaction of mitomycin C (MC) with fish sperm or calf thymus DNA immobilized onto carbon screen-printed electrodes (CSPE) and carbon paste electrode (CPE) have been studied by using electrochemical techniques as square wave voltammetry (SWV) and differential pulse voltammetry (DPV). After the interaction was occurred between DNA and MC on electrode surface, it was observed that the guanine signal was higher with bare electrode than DNA-modified one. The changes in the experimental parameters such as the concentration of MC, and the accumulation time of MC were studied by using SWV and DPV. In addition, reproducibility, and detection limit parameters were determined using both electrodes. The partition coefficient of MC was also calculated before and after interaction of MC with dsDNA at CPE surface. These results showed that these two different DNA biosensors could be used for the sensitive, rapid and cost effective detection of MC-DNA interaction.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Ege University, 35100 Bornova-Izmir, Turkey.
authors.forename: Dilsat
authors.initials: D
authors.lastname: Ozkan
authors.forename: Hakan
authors.initials: H
authors.lastname: Karadeniz
authors.forename: Arzum
authors.initials: A
authors.lastname: Erdem
authors.forename: Marco
authors.initials: M
authors.lastname: Mascini
authors.forename: Mehmet
authors.initials: M
authors.lastname: Ozsoz
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004563:Electrochemistry; D006147:Guanine / Q000737:chemistry / Q000378:metabolism*; D016685:Mitomycin / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction
chemical_list: D016685:Mitomycin; D006147:Guanine; D004247:DNA