NADH oxidation by mitochondria from the thermogenic plant Arum orientale.
The enzyme content of the mitochondrial respiratory chain was investigated in the heat-producing plant Arum orientale. It is shown that mitochondria isolated from thermogenic tissues of this plant (with respect to non-thermogenic tissues of A. orientale or to Zea mays) demonstrate significantly elevated levels of activities of two non-coupled NADH dehydrogenases oxidizing intramitochondrial and cytoplasmic NADH pools. It is postulated that operation of a completely non-coupled respiratory chain consisting of non-coupled NADH:quinone oxidoreductases and cyanide-resistant alternative quinol-oxidase is the main mechanism of heat production in thermogenic plants.
Bertsova
Yu V
YV
Department of Molecular Energetics of Microorganisms, Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119899, Russia.
Popov
V N
VN
Bogachev
A V
AV
D031058:Arum / Q000502:physiology*; D042967:Electron Transport Complex I / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins; D009243:NAD / Q000378:metabolism*; D009245:NADH Dehydrogenase / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D010940:Plant Proteins; D013053:Spectrophotometry; D022722:Thermogenesis / Q000502:physiology*; D000099188:Alternative Oxidase
D042967:Electron Transport Complex I; D024101:Mitochondrial Proteins; D009243:NAD; D009245:NADH Dehydrogenase; D010088:Oxidoreductases; D010940:Plant Proteins; D000099188:Alternative Oxidase