title: Potentiation by amino acid of the therapeutic effect of highly purified vitamin B2 in mice with lipopolysaccharide-induced shock.
abstract: The aim of this experiment was to clarify whether an amino acid supplement could enhance the therapeutic effect of vitamin B2 (riboflavin 5'-sodium phosphate; purity > 97%) in mice with lipopolysaccharide-induced shock. Six hours after injection of a lethal dose of lipopolysaccharide, treatment (6-h i.v. infusion) was commenced. All mice died in the groups treated with saline or aminolevane (an amino acids mixture used to treat hepatopathy); however, the survival rates in the vitamin B2 (10 mg/kg/6 h) and vitamin B2 plus aminolevane groups were 45% (P < 0.05) and 80% (P < 0.05), respectively. Valine (200 mg/kg/6 h) alone had little effect on the survival rate (10%), but the combination of vitamin B2 (10 mg/k/g/6 h) and valine was highly effective (80%, P < 0.05). Clinical trials of vitamin B2 plus amino acids for the treatment of patients with sepsis would appear to be warranted.
authors.affiliation: Tsukuba Research Laboratories, Eisai Co., Ltd., 5-1-3, Tokodai, Tsukuba 300-2635, Ibaraki, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Toyosawa
authors.forename: Mamoru
authors.initials: M
authors.lastname: Suzuki
authors.forename: Kohtarou
authors.initials: K
authors.lastname: Kodama
authors.forename: Seiichi
authors.initials: S
authors.lastname: Araki
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007262:Infusions, Intravenous; D007275:Injections, Intravenous; D015850:Interleukin-6 / Q000378:metabolism; D007564:Japan; D019344:Lactic Acid / Q000378:metabolism; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000009:adverse effects* / Q000737:chemistry; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D012256:Riboflavin / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D012772:Shock, Septic / Q000139:chemically induced* / Q000188:drug therapy* / Q000401:mortality; D013997:Time Factors; D014633:Valine / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*
chemical_list: D015850:Interleukin-6; D008070:Lipopolysaccharides; D019344:Lactic Acid; D014633:Valine; D005947:Glucose; D012256:Riboflavin