[Effects of total alkaloids of Tongbiling prescription on Th1 type cytokine expression in T lymphocytes].
OBJECTIVE
To study the effects of total alkaloid of Tongbiling prescription(TBL) on Th1 type cytokine expression in T cells in order to elucidate the anti-inflammatory mechanism of TBL.


METHODS
The lymphocytes were isolated from mouse mesenteric lymph nodes and cultured in-vitro. Various concentrations of TBL were added to the culture followed by phorbol ester and inomycin treatment and then incubated for another 4 hours. The expressions of IFN-gamma and TNF-alpha in the lymphocytes were analyzed by flow cytometry.


RESULTS
200 mg/L and 100 mg/L TBL could obviously inhibit IFN-gamma and TNF-alpha expressions in T lymphocytes.


CONCLUSIONS
Inhibiting Th1 cytokine expression may be one mechanism by which TBL can treat rheumatoid arthritis.
Li
Xiao-juan
XJ
Laboratory of Immunology, Guangzhou University of TCM, Guangzhou 510405, China. leexiaojuan@263.net
Chen
Guang-xing
GX
Liu
Liang
L
Wang
Pei-xun
PX
Hu
Ying-jie
YJ
Zeng
Yiao-ying
YY
Chen
Ji-fan
JF
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D001172:Arthritis, Rheumatoid / Q000188:drug therapy / Q000276:immunology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D007371:Interferon-gamma / Q000032:analysis*; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma