title: Utility of reverse transcriptase PCR for rapid diagnosis of influenza a virus infection and detection of amantadine-resistant influenza a virus isolates.
abstract: A reverse transcriptase PCR was developed to detect 50 or 5,000 RNA copies of influenza A virus per ml in throat swab specimens. The assay was more sensitive than the Directigen Flu A test. The technique was also used to detect amantadine-resistant isolates.
authors.affiliation: Department of Infectious Disease and Department of Laboratory Medicine and Pathology, Edward Hines Jr. Veterans Administration Hospital, 111-P, Hines, IL 60141, USA. constance.pachucki@med.va.gov
authors.forename: Constance T
authors.initials: CT
authors.lastname: Pachucki
authors.forename: M Aijaz
authors.initials: MA
authors.lastname: Khurshid
authors.forename: John
authors.initials: J
authors.lastname: Nawrocki
mesh_terms: D000547:Amantadine / Q000494:pharmacology*; D024882:Drug Resistance, Viral; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis*; D010614:Pharynx / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D000547:Amantadine