title: Application of electrospray ionization mass spectrometry in rapid typing of fengycin homologues produced by Bacillus subtilis.
abstract: OBJECTIVE
  To rapidly type the fengycin homologues produced by Bacillus subtilis strains with electrospray ionization/collision-induced dissociation (ESI/CID) mass spectrometry.
  
  
  RESULTS
  Fengycin homologues produced by Bacillus subtilis JA were analysed. When each homologue was subjected to ESI/CID analysis, ions representing characteristic fragmentations were detected. These ions can help to identify the homologues; even homologues of the same nominal mass can be discriminated by their ESI/CID spectra. Based on the CID results, fengycin homologues can be correctly assigned.
  ESI/CID leads to rapid detection and structural characterization of fengycin homologues or lipopeptides with similar properties. It will be very useful in studying the regulatory expression of these peptides.
authors.affiliation: Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Science, Hefei, Anhui, China. wangjun0457@sina.com
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Yao
authors.forename: Z
authors.initials: Z
authors.lastname: Yu
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry / Q000145:classification*; D015373:Bacterial Typing Techniques; D055666:Lipopeptides; D008074:Lipoproteins / Q000737:chemistry / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D055666:Lipopeptides; D008074:Lipoproteins; C049972:fengycin