title: Microautophagy and macropexophagy may occur simultaneously in Hansenula polymorpha.
abstract: We subjected methanol-grown cells of wild type Hansenula polymorpha simultaneously to nitrogen depletion and excess glucose conditions. Both treatments induce the degradation of peroxisomes, either selective (via excess glucose) or non-selective (via nitrogen limitation). Our combined data strongly suggest that both processes occur simultaneously under these conditions. The implications of these findings on studies of autophagy and related transport pathways to the vacuole in yeast are discussed.
authors.affiliation: Laboratory of Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, P.O. Box 14, 9750 AA Haren, The Netherlands.
authors.forename: Iryna
authors.initials: I
authors.lastname: Monastryska
authors.forename: Klaas
authors.initials: K
authors.lastname: Sjollema
authors.forename: Ida J
authors.initials: IJ
authors.lastname: van der Klei
authors.forename: Jan A K W
authors.initials: JA
authors.lastname: Kiel
authors.forename: Marten
authors.initials: M
authors.lastname: Veenhuis
mesh_terms: D001343:Autophagy; D009584:Nitrogen / Q000378:metabolism; D010843:Pichia / Q000378:metabolism / Q000502:physiology*
chemical_list: D009584:Nitrogen