TFF2 (trefoil family factor2) inhibits apoptosis in breast and colorectal cancer cell lines.
We have recently demonstrated that human TFF2 inhibits apoptosis in the non-TFF2 expressing breast adenocarcinoma cell line MCF-7. In this study we examined the impact of TFF2 and an anti-TFF2 antibody (hSP3) on the survival of other human adenocarcinoma cell lines; TFF2-positive (LS174T and SW480) and TFF2-negative (MCF-7 and T47D). Addition of TFF2 protected the (TFF2-) lines but had no effect on those constitutively expressing TFF2. Blocking with hSP3 significantly increased apoptosis in the (TFF2+) cell lines with minimal effect on the (TFF2-) cells. Our results show that the cytoprotective effect of TFF2 seen in MCF-7 cells is not cell line-specific and can be abrogated by inhibition of its expression.
Siu
Lai-San
LS
Department of Histopathology (L-SS, HR, GWHS, ENL), Imperial College, Hammersmith Campus, London W12 0NN, UK.
Romanska
Hanna
H
Abel
Paul D
PD
Baus-Loncar
Mirela
M
Kayademir
Tuncay
T
Stamp
Gordon W H
GW
Lalani
El-Nasir
el-N
D000906:Antibodies / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism*; D006801:Humans; D009077:Mucins / Q000494:pharmacology*; D009124:Muscle Proteins / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D000071164:Trefoil Factor-2; D014407:Tumor Cells, Cultured
D000906:Antibodies; D009077:Mucins; D009124:Muscle Proteins; D010455:Peptides; D011994:Recombinant Proteins; C000604007:TFF2 protein, human; D000071164:Trefoil Factor-2