title: Fatty acid transport in adipocytes monitored by imaging intracellular free fatty acid levels.
abstract: Transport of free fatty acids (FFA) across the adipocyte plasma membrane is critical for maintaining homeostasis. To determine the membrane's role in regulating transport we describe here the first measurements of the intracellular (unbound) FFA concentration ([FFA(i)]) and their use in monitoring transport of FFA across 3T3F442A adipocytes. [FFA(i)] was measured by microinjecting cells with ADIFAB, a fluorescently labeled fatty acid-binding protein that is used to measure unbound FFA levels. We used ratio fluorescence microscopy of intracellular ADIFAB to image unbound FFA levels and determined the time course of [FFA(i)] in response to changing the extracellular unbound FFA concentration ([FFA(o)]). [FFA(o)] was clamped at defined levels using complexes of FFA and bovine serum albumin. We show that FFA influx is slow, requiring about 300 s to reach steady state (rate constant approximately 0.02 s(-1)) and saturable (K(o) approximately 200 nm). Efflux is twice as fast as influx, for zero [FFA(o)], but decreases with increasing [FFA(o)]. Surprisingly, at steady state [FFA(i)] is 2-5-fold (average 2-fold) greater than [FFA(o)] and this [FFA(i)]/[FFA(o)] gradient is abolished by depleting cellular ATP. Our results indicate that FFA transport across adipocyte membranes is highly regulated, involving an ATP-driven pump and a mechanism for gating efflux that is sensitive to [FFA(o)]. These characteristics are well described by a membrane carrier model but are not consistent with FFA transport across the membrane's lipid phase. We suggest that these characteristics are important in regulating circulating FFA levels by the adipocyte.
authors.affiliation: Torrey Pines Institute for Molecular Studies, San Diego, California 92121, USA.
authors.forename: J Patrick
authors.initials: JP
authors.lastname: Kampf
authors.forename: Alan M
authors.initials: AM
authors.lastname: Kleinfeld
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins; D002460:Cell Line; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism*; D005453:Fluorescence; D007091:Image Processing, Computer-Assisted; D051379:Mice; D008845:Microinjections; D011994:Recombinant Proteins
chemical_list: D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D011994:Recombinant Proteins; C079339:acrylodated intestinal fatty acid binding protein, recombinant