Quantification of intra- and extra-cellular thermophilic lipase/esterase production by Thermus sp.
Four Thermus strains produced lipolytic activity when grown in liquid medium for 30 h at 70 degrees C. The highest total lipase/esterase activity (57 U l(-1)) was in Thermus aquaticus YT-1, followed by Thermus thermophilus HB27 and HB8 (33 and 25 U l(-1), respectively), and finally by Thermus sp. (16 U l(-1)). Extra-cellular activity was detected in T. aquaticus YT-1 and T. thermophilus HB27 (33 and 17 U l(-1)). All enzymes were stable at 80 degrees C over 30 min, and their activity towards fatty acid esters increased as substrate chain-length diminished (i.e. hydrolysis rate was up to 6-fold higher on p-nitrophenyl caproate than on laurate).
Domínguez
A
A
Department of Chemical Engineering, University of Vigo, 36200 Vigo, Spain.
Sanromán
A
A
Fuciños
P
P
Rúa
M L
ML
Pastrana
L
L
Longo
M A
MA
D001709:Biotechnology / Q000379:methods; D049109:Cell Proliferation; D004950:Esterases / Q000737:chemistry; D004952:Esters; D005227:Fatty Acids / Q000737:chemistry; D006868:Hydrolysis; D008049:Lipase / Q000737:chemistry; D013379:Substrate Specificity; D013696:Temperature; D013824:Thermus / Q000201:enzymology*; D016963:Thermus thermophilus / Q000201:enzymology; D013997:Time Factors
D004952:Esters; D005227:Fatty Acids; D004950:Esterases; D008049:Lipase