[The study on correlation of c-myc gene expression with vascular smooth muscle cell proliferation in patients with portal hypertension].
OBJECTIVE
To investigate c-myc proto-oncogene expression and the relationship of PCNA protein expression of extrahepatic vascular smooth muscle cell in patients with portal hypertension and normal vessels.


METHODS
RT-PCR was used to demonstrate the expression of c-myc mRNA and immuno-chemistry strain was performed to detect the expression of PCNA protein in splenic veins of 28 patients with portal hypertension and 12 normal vessels.


RESULTS
The straining of PCNA protein was (29.8 +/- 4.7)% in splenic veins with portal hypertension, Normal vessels did not detect PCNA protein expression (P < 0.01); RT-PCR showed that the expression of c-myc mRNA in PCNA-positive control and in negative control of splenic veins with portal hypertension were (7.61 +/- 1.04)% and (3.82 +/- 0.92)%, respectively. There ws different between two groups (P < 0.01) and significant different (P < 0.01) when compared with (1.01 +/- 0.21)% in normal vessels.


CONCLUSIONS
The c-myc was immediate-early gene when it modulated proliferation of vascular smooth muscle cell. Hemodynamic disturbance of portal vein system activate the proto-oncogene of smooth muscle cells in splenic vein of patients with portal hypertension, promoting the proliferation, migrating and phenotypic change and resulting in vascular remodelling of splenic veins.
Zeng
Jin-hua
JH
Department of General surgery, Tongji Hospital, Tongji Medical College of Hua Zhong University of Science and Technology, Wuhan 430030, China.
Yang
Zhen
Z
Xu
Jun
J
D000328:Adult; D000368:Aged; D049109:Cell Proliferation; D005260:Female; D015870:Gene Expression; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D006975:Hypertension, Portal / Q000235:genetics* / Q000473:pathology; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger / Q000235:genetics; D013162:Splenic Vein / Q000166:cytology / Q000378:metabolism
C000715470:MAS1 protein, human; D018809:Proliferating Cell Nuclear Antigen; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger