title: Bacillus thuringiensis serovar shandongiensis strain 89-T-34-22 produces multiple cytotoxic proteins with similar molecular masses against human cancer cells.
abstract: OBJECTIVE
  To prove that Bacillus thuringiensis serovar shandongiensis strain 89-T-34-22 produces several novel cytotoxic proteins against human leukaemic T cells.
  
  
  RESULTS
  Parasporal inclusion protein was solubilized and processed by proteinase K and was separated by anion-exchange chromatography. Cytopathic effects of each fraction against MOLT-4 and Jurkat cells were monitored.
  
  
  CONCLUSIONS
  Existence of at least two novel cytotoxic proteins was suggested and N-terminal sequences of the newly identified proteins were determined to be QSTTDVIREY and X (Y or I) (P or I) NLANELA (X indicates uncertain amino acids). Molecular masses of the two proteins were approx. 27-28 kDa.
  
  
  CONCLUSIONS
  In this study, we demonstrated that the strain 89-T-34-22 produces at least two novel cytotoxic proteins with similar molecular masses against human cancer cells. This is the first strain of B. thuringiensis which produces multiple cytotoxic proteins against human cancer cells.
authors.affiliation: Fukuoka Industrial Technology Center, Fukuoka, Japan. sokumura@fitc.pref.fukuoka.jp
authors.forename: S
authors.initials: S
authors.lastname: Okumura
authors.forename: T
authors.initials: T
authors.lastname: Akao
authors.forename: K
authors.initials: K
authors.lastname: Higuchi
authors.forename: H
authors.initials: H
authors.lastname: Saitoh
authors.forename: E
authors.initials: E
authors.lastname: Mizuki
authors.forename: M
authors.initials: M
authors.lastname: Ohba
authors.forename: K
authors.initials: K
authors.lastname: Inouye
mesh_terms: D000595:Amino Acid Sequence; D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D001413:Bacillus thuringiensis / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D003603:Cytotoxins / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D019169:Jurkat Cells; D015458:Leukemia, T-Cell; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D001426:Bacterial Proteins; D003603:Cytotoxins