[Experimental study on the effect of VEGF165 antisense RNA on human squamous cell carcinoma of esophagus].
OBJECTIVE
To investigate the effect of antisense RNA against vascular endothelial growth factor 165 (VEGF165) on human esophagus squamous cell carcinoma cell line EC109.


METHODS
Eukaryotic expression vector for VEGF165 antisense RNA was constructed and identified. Recombinant plasmid was transfected into EC109 cells and the transfected EC109 cells were inoculated subcutaneously to nude mice. The biological characteristics and tumorigenicity of transfected EC109 cells were observed by in situ hybridization, laser confocal microscope, transmission electron microscopy and flow cytometry.


RESULTS
The eukaryotic expression vector pCEP-AVEGF165 was successfully constructed and expressed in transfected EC109 cells. The rate of VEGF165 expression dropped by 75% in transfected cells. The morphology and cell cycle of transfected EC109 cells were not affected by the antisense RNA, but the tumorigenicity and angiogenesis of transfected EC109 cells were greatly reduced in nude mice. The volume of tumors in pCEP-AVEGF165 transfected group, empty vector transfected group and control group were (820+/-112.5) mm3, (7 930+/-1 035) mm3 and (7 850+/-950) mm3, respectively. The microvessel density of the three groups were (8.5+/-1.2)/mm2, (44.3+/-9.4)/mm2 and (46.4+/-12.6)/mm2, respectively.


CONCLUSIONS
The angiogenesis and tumorigenicity of human esophageal squamous cell carcinoma were effectively inhibited by VEGF165 antisense RNA, which may be applied to treat solid tumor in the future.
Gu
Zhong-ping
ZP
Department of Thoracic Surgery, Fourth Military Medical University, Xi'an 710038, China. zhongpg@pub.xaonline.com
Wang
Yun-jie
YJ
Zhou
Yong-an
YA
Liu
Kun
K
Cheng
Qing-shu
QS
Li
Jin-ge
JG
Bai
Xue-fan
XF
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D004938:Esophageal Neoplasms / Q000378:metabolism / Q000473:pathology*; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic / Q000517:prevention & control*; D010957:Plasmids; D016372:RNA, Antisense / Q000378:metabolism / Q000502:physiology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000378:metabolism
D016372:RNA, Antisense; D011994:Recombinant Proteins; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A