Use of a recombinant envelope protein subunit antigen for specific serological diagnosis of West Nile virus infection.
Serological diagnosis of West Nile virus (WNV) infection is complicated by extensive antigenic cross-reactivity with other closely related flaviviruses, such as St. Louis encephalitis virus. Here we describe a recombinant, bacterially expressed antigen equivalent to structural domain III of the WNV envelope protein that has allowed clear discrimination of antibody responses to WNV from those against other related flaviviruses in indirect enzyme-linked immunosorbent assays using standardized control antisera and field-collected samples.
Beasley
David W C
DW
Department of Pathology, UTMB, 301 University Blvd., Galveston, TX 77555-0609, USA.
Holbrook
Michael R
MR
Travassos Da Rosa
Amelia P A
AP
Coffey
Lark
L
Carrara
Anne-Sophie
AS
Phillippi-Falkenstein
Kathrine
K
Bohm
Rudolf P
RP
Ratterree
Marion S
MS
Lillibridge
Kristy M
KM
Ludwig
George V
GV
Estrada-Franco
Jose
J
Weaver
Scott C
SC
Tesh
Robert B
RB
Shope
Robert E
RE
Barrett
Alan D T
AD
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D021122:Protein Subunits; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D012698:Serologic Tests; D014901:West Nile Fever / Q000175:diagnosis*; D014902:West Nile virus / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D021122:Protein Subunits; D011994:Recombinant Proteins