title: IgG reactivity with a 100-kDa tissue and endothelial cell antigen identified as topoisomerase 1 distinguishes between limited and diffuse systemic sclerosis patients.
abstract: We have analyzed antibody (Ab) reactivities of patients with limited systemic sclerosis (SSc) and anti-centromere Ab, patients with diffuse SSc and anti-topoisomerase 1 (anti-topo 1) Ab, patients with diffuse SSc without anti-topo 1 or anti-centromere Ab and age- and gender-matched healthy controls with normal human tissue and endothelial cell (EC) antigens. IgG reactivities with tissue antigens differed significantly between patients with anti-topo 1 Ab and patients with anti-centromere Ab. One 100-kDa band identified as topoisomerase 1 in macrovascular and microvascular EC extracts was recognized by IgG from patients with anti-topo 1 Ab and 50% of patients without specific Ab. IgG from patients with limited SSc and anti-centromere Ab, but not those of other patients or controls specifically recognized a 80-kDa band only in microvascular EC. Our results indicate that Ab from patients with limited or diffuse SSc with or without anti-topo 1 Ab exhibit specific and mutually exclusive reactivity patterns.
authors.affiliation: UPRES EA 3408, UFR-SMBH Léonard-de-Vinci, Bobigny, France.
authors.forename: Paloma
authors.initials: P
authors.lastname: García de la Peña-Lefebvre
authors.forename: Youri
authors.initials: Y
authors.lastname: Chanseaud
authors.forename: Mathieu C
authors.initials: MC
authors.lastname: Tamby
authors.forename: Joseph
authors.initials: J
authors.lastname: Reinbolt
authors.forename: Frédéric
authors.initials: F
authors.lastname: Batteux
authors.forename: Yannick
authors.initials: Y
authors.lastname: Allanore
authors.forename: André
authors.initials: A
authors.lastname: Kahan
authors.forename: Olivier
authors.initials: O
authors.lastname: Meyer
authors.forename: Olivier
authors.initials: O
authors.lastname: Benveniste
authors.forename: Olivier
authors.initials: O
authors.lastname: Boyer
authors.forename: Loïc
authors.initials: L
authors.lastname: Guillevin
authors.forename: Marie-Christophe
authors.initials: MC
authors.lastname: Boissier
authors.forename: Luc
authors.initials: L
authors.lastname: Mouthon
mesh_terms: D001323:Autoantibodies / Q000032:analysis*; D015153:Blotting, Western; D002503:Centromere / Q000276:immunology; D004264:DNA Topoisomerases, Type I / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D042783:Endothelial Cells / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008297:Male; D008875:Middle Aged; D012595:Scleroderma, Systemic / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D004264:DNA Topoisomerases, Type I