Kinetics of improved productivity of beta-galactosidase by a cycloheximide-resistant mutant of Kluyveromyces marxianus.
The maximum volumetric productivity of beta-galactosidase by a Kluyveromyces marxianus mutant, grown on lactose/corn steep liquor medium for 3 d, was 150 IU l(-1) h(-1) which is twice that of the parent organism. During product formation, mutated cells provided more resistance against thermal inactivation.
Rajoka
M I
MI
National Institute for Biotechnology and Genetic Engineering, P.O. Box 577, Jhang Road, Faisalabad, Pakistan. mirajoka@nibge.org
Latif
F
F
Khan
S
S
Shahid
Riaz
R
D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003513:Cycloheximide / Q000494:pharmacology*; D019277:Entropy; D005285:Fermentation; D006358:Hot Temperature; D007700:Kinetics; D007716:Kluyveromyces / Q000235:genetics*; D009154:Mutation; D011134:Polysaccharides / Q000737:chemistry; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D013696:Temperature; D013816:Thermodynamics; D013997:Time Factors; D001616:beta-Galactosidase / Q000737:chemistry*
D011134:Polysaccharides; D011500:Protein Synthesis Inhibitors; D002244:Carbon; D003513:Cycloheximide; D001616:beta-Galactosidase