Instability of PRV-1 mRNA: a factor to be considered in PRV-1 quantification for the diagnosis of polycythemia vera.
High expression of PRV-1 mRNA in granulocytes has been proposed as a new diagnostic marker for polycythemia vera. We used real-time reverse transcription polymerase chain reaction (RT-PCR) to measure the levels of PRV-1 mRNA, GAPDH mRNA and 18S rRNA in granulocytes obtained from blood samples processed 2, 24 and 48 hours after collection and observed a significant decrease of PRV-1 levels after 24 and 48 hours. The instability of PRV-1 mRNA may affect the diagnostic value of the PRV-1 test in blood samples stored for extended periods.
Jelinek
Jaroslav
J
Jedlickova
Katerina
K
Guan
Yongli
Y
Prchal
Josef T
JT
D003951:Diagnostic Errors; D058851:GPI-Linked Proteins; D006098:Granulocytes / Q000737:chemistry; D006801:Humans; D007519:Isoantigens / Q000032:analysis / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000032:analysis / Q000235:genetics*; D011087:Polycythemia Vera / Q000175:diagnosis*; D020871:RNA Stability; D012333:RNA, Messenger / Q000032:analysis; D011956:Receptors, Cell Surface / Q000032:analysis / Q000235:genetics*
C407242:CD177 protein, human; D058851:GPI-Linked Proteins; D007519:Isoantigens; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface