[Expression of MAGE-1 and MAGE-3 genes in gastric cancer and gastric biopsy tissues and its clinical significance].
OBJECTIVE
To detect the expression of melanoma antigen-1 and -3 genes (MAGE-1 and MAGE-3) and explore its significance in immunotherapeutic application.


METHODS
The expression of MAGE-1 and MAGE-3 in gastric cancer, para-tumorous normal mucosa, and gastric biopsy tissues were detected by RT-PCR. The target genes in RT-PCR product were verified by means of DNA sequencing.


RESULTS
MAGE-1 expression was positive in 26 out of 36 (72.22%) cases of gastric cancer, MAGE-3 positive in 25 out of 36(69.44%), and both MAGE-1 and MAGE-3 positive in 19 out of 36 (52.78%). Positive expression rates of MAGE-1, MAGE-3 and both MAGE-1 and MAGE-3 were 47.22%, 44.44% and 30.55%, respectively in para-tumorous normal mucosa, while 29.73%, 27.03% and 8.1% in gastric biopsy tissues The positive rates of MAGE-1 and MAGE-3 gene expressions in tumor tissues was significant higher than those its parallelism mucosae and biopsy tissues from chronic gastritis. The expressions of MAGE-1 and MAGE-3 genes in tumor tissues were relevant to intruded depth and the differentiated degree of tumor cells (P<0.05).


CONCLUSIONS
MAGE-1 and MAGE-3 protein may be used as targets molecules of immunotherapy for gastric cancer and indexes for follow-up study of chronic gastritis as well.
Chen
Xue-hua
XH
Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China. xhchensh@yahoo.com
Liu
Bing-ya
BY
Zhang
Dong-qing
DQ
Zhang
Yi
Y
Zhang
Yi
Y
Li
Jian-fang
JF
Zhu
Zheng-gang
ZG
D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D005753:Gastric Mucosa / Q000378:metabolism*; D005756:Gastritis / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D058950:Melanoma-Specific Antigens; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009367:Neoplasm Staging; D009857:Oncogenes; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology
D000951:Antigens, Neoplasm; C072011:MAGEA1 protein, human; C072013:MAGEA3 protein, human; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins