Conversion of aquifex aeolicus 3-deoxy-d-manno-octulosonate 8-phosphate synthase, a metalloenzyme, into a nonmetalloenzyme.
The Aquifex aeolicus 3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS), a class II metalloenzyme, is converted into an active nonmetalloenzyme by a single amino acid mutation, namely, C11N. The result may provide insight into the evolutionary link between the two KDO8PS classes as well as the potential role of the metal and/or asparagine in the catalytic mechanism.
Li
Jingjing
J
Department of Medicinal Chemistry and Chemistry, University of Michigan, Ann Arbor, Michigan 48109-1065, USA.
Wu
Jing
J
Fleischhacker
Angela S
AS
Woodard
Ronald W
RW
D000446:Aldehyde-Lyases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D019943:Amino Acid Substitution; D001216:Asparagine / Q000737:chemistry; D001419:Bacteria / Q000201:enzymology*; D002384:Catalysis; D004926:Escherichia coli / Q000201:enzymology; D008670:Metals / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D009154:Mutation
D008670:Metals; D001216:Asparagine; C020011:2-dehydro-3-deoxyphosphooctonate aldolase; D000446:Aldehyde-Lyases