title: Trans10,cis12-18:2 is a more potent inhibitor of de novo fatty acid synthesis and desaturation than cis9,trans11-18:2 in the mammary gland of lactating mice.
abstract: To investigate the effects of 2 conjugated linoleic acid (CLA) isomers and trans11-18:1 (TVA) on de novo lipogenesis and desaturation in liver and mammary gland, lactating mice were fed diets containing 3% canola oil (control) or 2% canola oil plus 1% stearic acid (SA), TVA, cis9,trans11 CLA (c9t11), or trans10,cis12 CLA (t10c12). In mammary tissue, TVA and CLA isomers reduced mRNA for acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS) compared with control, but only c9t11 and t10c12 reduced mammary ACC activity. Of the 2 CLA isomers, t10c12 caused a greater reduction in mammary ACC activity. Hepatic ACC or FAS activity and mRNA abundance were not affected by dietary treatments. Feeding TVA, c9t11, or t10c12 reduced mammary stearoyl-CoA desaturase 1 (SCD) mRNA and activity. Reduction was greater due to feeding t10c12 compared with c9t11. Hepatic SCD mRNA was not affected by dietary treatments, but both CLA isomers depressed hepatic SCD activity. Results indicated that t10c12 is a more potent inhibitor of mammary lipogenesis and desaturation than is c9t11. A net gain of 77 and 1690 micro g of c9t11 in liver and mammary tissue, respectively, was found in the TVA-fed group over the control and SA-fed group. However, reduced mammary SCD mRNA or activity due to feeding TVA may indicate a limited capacity for desaturation of dietary TVA to c9t11 in vivo.
authors.affiliation: Dairy Science Department, Virginia Tech, Blacksburg, VA 24061-0315, USA. xlin@im.wustl.edu
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Lin
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Loor
authors.forename: Joseph H
authors.initials: JH
authors.lastname: Herbein
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000235:genetics; D000818:Animals; D044943:Fatty Acid Desaturases / Q000037:antagonists & inhibitors*; D064429:Fatty Acid Synthases / Q000235:genetics; D005227:Fatty Acids / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000378:metabolism; D005260:Female; D007527:Isoenzymes / Q000235:genetics; D007536:Isomerism; D007774:Lactation / Q000378:metabolism*; D044243:Linoleic Acids, Conjugated / Q000378:metabolism / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D009829:Oleic Acids / Q000378:metabolism / Q000494:pharmacology*; D009994:Osmolar Concentration; D012333:RNA, Messenger / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase / Q000235:genetics
chemical_list: D005227:Fatty Acids; D007527:Isoenzymes; D044243:Linoleic Acids, Conjugated; D009829:Oleic Acids; D012333:RNA, Messenger; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; D064429:Fatty Acid Synthases; D000103:Acetyl-CoA Carboxylase; C050413:11-octadecenoic acid