title: A wide-range integrative yeast expression vector system based on Arxula adeninivorans-derived elements.
abstract: An Arxula adeninivorans integration vector was applied to a range of alternative yeast species including Saccharomyces cerevisiae, Debaryomyces hansenii, Debaryomyces polymorphus, Hansenula polymorpha and Pichia pastoris. The vector harbours a conserved A. adeninivorans-derived 25S rDNA sequence for targeting, the A. adeninivorans-derived TEF1 promoter for expression control of the reporter sequence, and the Escherichia coli-derived hph gene conferring resistance against hygromycin B for selection of recombinants. Heterologous gene expression was assessed using a green fluorescent protein (GFP) reporter gene. The plasmid was found to be integrated into the genome of the various hosts tested; recombinant strains of all species exhibited heterologous gene expressions of a similar high level.
authors.affiliation: Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstrasse 3, 06466 Gatersleben, Germany.
authors.forename: Yaroslav
authors.initials: Y
authors.lastname: Terentiev
authors.forename: Almudena Huarto
authors.initials: AH
authors.lastname: Pico
authors.forename: Erik
authors.initials: E
authors.lastname: Böer
authors.forename: Thomas
authors.initials: T
authors.lastname: Wartmann
authors.forename: Jens
authors.initials: J
authors.lastname: Klabunde
authors.forename: Uta
authors.initials: U
authors.lastname: Breuer
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Babel
authors.forename: Manfred
authors.initials: M
authors.lastname: Suckow
authors.forename: Gerd
authors.initials: G
authors.lastname: Gellissen
authors.forename: Gotthard
authors.initials: G
authors.lastname: Kunze
mesh_terms: D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D004718:Saccharomycetales / Q000235:genetics*; D014170:Transformation, Genetic; D015003:Yeasts / Q000235:genetics* / Q000378:metabolism
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins