title: Expression profiling: toward an application in sepsis diagnostics.
abstract: Sepsis is a common and serious health problem whereby improvements in diagnosis are crucial in increasing survival rates. To test whether profiling transcription is applicable to sepsis diagnosis, we analyzed whole blood using a microarray containing probes for 340 genes relevant to inflammation. The patient's gene expression pattern was highly homogenous, resulting in 69% of differentially expressed genes. With a positive predictive value of 98%, a list of 50 differentially expressed genes was compiled, and randomly chosen transcripts were confirmed by PCR. Here, we present the first evidence that microarrays can identify typical gene expression profiles in the blood of patients with severe sepsis. Regardless of the heterogeneity of the patients, we observed a striking correlation between the conventional diagnostic classification and our approach. The unity of responses suggests that the principle of this multiparameter approach can be adapted to early stage sepsis diagnosis.
authors.affiliation: Hospital Na Homolce, Prague, Czech Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Prucha
authors.forename: Andriy
authors.initials: A
authors.lastname: Ruryk
authors.forename: Hinnerk
authors.initials: H
authors.lastname: Boriss
authors.forename: Eva
authors.initials: E
authors.lastname: Möller
authors.forename: Roman
authors.initials: R
authors.lastname: Zazula
authors.forename: Ivan
authors.initials: I
authors.lastname: Herold
authors.forename: Ralf A
authors.initials: RA
authors.lastname: Claus
authors.forename: Konrad A
authors.initials: KA
authors.lastname: Reinhart
authors.forename: Peter
authors.initials: P
authors.lastname: Deigner
authors.forename: Stefan
authors.initials: S
authors.lastname: Russwurm
mesh_terms: D018806:APACHE; D000328:Adult; D000368:Aged; D001483:Base Sequence; D004247:DNA / Q000097:blood / Q000235:genetics; D017931:DNA Primers; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D007249:Inflammation / Q000235:genetics; D008297:Male; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012769:Shock / Q000175:diagnosis* / Q000235:genetics*; D012772:Shock, Septic / Q000175:diagnosis / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D004247:DNA