title: Measurement of nitric oxide by reconversion of nitrate/nitrite to NO.
abstract: A simple and sensitive method is presented to measure the unstable molecule nitric oxide (NO) by reconversion of nitrate/nitrite to NO. Nitrate and nitrite are the stable degradation products of NO that accumulate in supernatants of biological samples that release nitric oxide. First, nitrate is enzymatically converted to nitrite using nitrate reductase. In a second step, nitrite is reduced to equimolar NO concentrations by an acidic iodide solution and quantified with an amperometric Clark-type electrode. This method provides the ability to assess basal-and agonist-stimulated cumulative NO formation in different biological models and is a sensitive alternative to the widely used Griess assay.
authors.affiliation: Institute of Pharmacology, University of Cologne, Germany.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Berkels
authors.forename: Svenja
authors.initials: S
authors.lastname: Purol-Schnabel
authors.forename: Renate
authors.initials: R
authors.lastname: Roesen
mesh_terms: D000818:Animals; D003331:Coronary Vessels / Q000378:metabolism*; D004566:Electrodes; D004730:Endothelium, Vascular / Q000378:metabolism*; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000378:metabolism; D009566:Nitrates / Q000032:analysis* / Q000737:chemistry; D009569:Nitric Oxide / Q000032:analysis* / Q000737:chemistry; D009573:Nitrites / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D013552:Swine
chemical_list: D009566:Nitrates; D009573:Nitrites; D009569:Nitric Oxide; D009565:Nitrate Reductases; D050901:Nitrate Reductase