title: Ex vivo phenotype and frequency of influenza virus-specific CD4 memory T cells.
abstract: Recent advances in class II tetramer staining technology have allowed reliable direct ex vivo visualization of antigen-specific CD4 T cells. In order to define the frequency and phenotype of a prototype response to a nonpersistent pathogen, we have used such techniques to analyze influenza virus-specific memory CD4 T cells directly from blood. These responses are stably detectable ex vivo at low frequencies (range, 0.00012 to 0.0061% of CD4 T cells) and display a distinct "central memory" CD62L(+) phenotype.
authors.affiliation: Nuffield Department of Medicine, University of Oxford, United Kingdom.
authors.forename: Michaela
authors.initials: M
authors.lastname: Lucas
authors.forename: Cheryl L
authors.initials: CL
authors.lastname: Day
authors.forename: Jessica R
authors.initials: JR
authors.lastname: Wyer
authors.forename: Sharon L
authors.initials: SL
authors.lastname: Cunliffe
authors.forename: Andrew
authors.initials: A
authors.lastname: Loughry
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: McMichael
authors.forename: Paul
authors.initials: P
authors.lastname: Klenerman
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D005802:Genes, MHC Class II; D006684:HLA-DR Antigens; D006801:Humans; D007156:Immunologic Memory; D019041:L-Selectin / Q000378:metabolism*; D009975:Orthomyxoviridae / Q000276:immunology*; D010641:Phenotype
chemical_list: D006684:HLA-DR Antigens; D019041:L-Selectin