Use of paraffin-embedded tissue for identification of Saccharomyces cerevisiae in a baker's lung nodule by fungal PCR and nucleotide sequencing.
A 40-year-old healthy male employed in a bakery presented with a single lung nodule and underwent investigations to rule out pulmonary carcinoma. Biopsy was positive for yeast cells, which did not match common fungal pathogens. PCR assay of paraffin-embedded tissue and nucleotide sequencing with ribosomal ITS1-ITS2 universal primers revealed the presence of Saccharomyces cerevisiae.
Ren
Ping
P
Mycology Laboratory, Wadsworth Center, New York State Department of Health, SUNY School of Public Health, University at Albany, Albany, NY 12201-2002, USA.
Sridhar
Sundara
S
Chaturvedi
Vishnu
V
D000328:Adult; D001483:Base Sequence; D006801:Humans; D008168:Lung / Q000382:microbiology*; D008297:Male; D008969:Molecular Sequence Data; D016273:Occupational Exposure; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA