[Establishment and characterization of human alloantigen induced T cell clones].
OBJECTIVE
To estabilish and analyze stable human T cell clones cultured in-vitro.


METHODS
The activated T cells from mixed lymphocyte culture (MLC) were cloned by limiting dilution using irradiated PBMCs as feeder cells. T cell clones were then characterized by immunofluorescence staining and flow cytometry analysis.


RESULTS
16 clones were obtained, of which all but one were alphabetaT cells. Among the 15 alphabetaT cell clones, 9 clones belonged to Th subset, including 3 Th0 and 6 Th2, and 6 clones belonged to Tc subset, including 5 Tc0 and 1 Tc2.


CONCLUSIONS
Fifteen human T cell clones were successfully established, which lays the foundation for study on markers and function of Tc1 and Tc2 subsets.
Han
Wei-ning
WN
Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.
Cao
Yun-xin
YX
Zhang
Yun
Y
Li
Qi
Q
Xie
Xin
X
Liu
Xue-song
XS
Jin
Bo-quan
BQ
D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D015789:HLA-A2 Antigen / Q000032:analysis; D006801:Humans; D007519:Isoantigens / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D000098862:T Lineage-Specific Activation Antigen 1
D000945:Antigens, Differentiation, T-Lymphocyte; D000098862:T Lineage-Specific Activation Antigen 1; D015789:HLA-A2 Antigen; D007519:Isoantigens