Enzyme activities in some types of peripheral leukocytes of thoroughbred race horses before and after the races.
Glucose, free fatty acid (FFA), triglyceride (TG) and immunoreactive insulin (IRI) concentrations in plasma and activities of enzymes related to energy metabolism in some types of peripheral leukocytes were measured in thoroughbred race horses before and after racing. Glucose, FFA, TG and IRI concentrations and enzyme activities did not change significantly in plasma. However, the activities of cytosolic hexokinase, malate dehydrogenase (MDH), mitochondrial MDH and aspartate aminotransferase decreased significantly in leukocytes of the horses after the races. The cytosolic ratio of MDH/LDH activity (ML ratio) in leukocytes decreased significantly after racing, and the ratio may be a useful indicator to evaluate energy metabolism in race horses.
Hosoya
M
M
Division of Veterinary Biochemistry, Department of Veterinary Science, Nippon Veterinary and Animal Science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan.
Inoue
A
A
Kimura
N
N
Arai
T
T
D000594:Amino Acid Oxidoreductases / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003600:Cytosol / Q000201:enzymology; D004734:Energy Metabolism / Q000502:physiology; D004798:Enzymes / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D006736:Horses / Q000097:blood*; D007328:Insulin / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007962:Leukocytes / Q000166:cytology / Q000201:enzymology*; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D010805:Physical Conditioning, Animal; D014280:Triglycerides / Q000097:blood
D001786:Blood Glucose; D004798:Enzymes; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D014280:Triglycerides; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D000594:Amino Acid Oxidoreductases; C025745:aspartate dehydrogenase; D006593:Hexokinase; D005952:Glucose-6-Phosphatase