Unilamellar vesicles as potential capreomycin sulfate carriers: preparation and physicochemical characterization.
The aim of this work was to evaluate unilamellar liposomes as new potential capreomycin sulfate (CS) delivery systems for future pulmonary targeting by aerosol administration. Dipalmitoylphosphatidylcholine, hydrogenated phosphatidylcholine, and distearoylphosphatidylcholine were used for liposome preparation. Peptide-membrane interaction was investigated by differential scanning calorimetry (DSC) and attenuated total internal reflection Fourier-transform infrared spectroscopy (ATIR-FTIR). Peptide entrapment, size, and morphology were evaluated by UV spectrophotometry, photocorrelation spectroscopy, and transmission electron microscopy, respectively. Interaction between CS and the outer region of the bilayer was revealed by DSC and ATIR-FTIR. DSPC liposomes showed enhanced interdigitation when the CS molar fraction was increased. Formation of a second phase on the bilayer surface was observed. From kinetic and permeability studies, CS loaded DSPC liposomes resulted more stable if compared to DPPC and HPC over the period of time investigated. The amount of entrapped peptide oscillated between 10% and 13%. Vesicles showed a narrow size distribution, from 138 to 166 nm, and a good morphology. These systems, in particular DSPC liposomes, could represent promising carriers for this peptide.
Giovagnoli
Stefano
S
Department of Chemistry and Technology of Drugs, Università degli Studi di Perugia, 06123 Perugia, Italy.
Blasi
Paolo
P
Vescovi
Claudia
C
Fardella
Giuseppe
G
Chiappini
Ione
I
Perioli
Luana
L
Ricci
Maurizio
M
Rossi
Carlo
C
D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D002207:Capreomycin / Q000008:administration & dosage* / Q000737:chemistry; D004337:Drug Carriers; D016503:Drug Delivery Systems; D004355:Drug Stability; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry*; D010316:Particle Size; D010539:Permeability; D017550:Spectroscopy, Fourier Transform Infrared; D013678:Technology, Pharmaceutical
D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D008081:Liposomes; D002207:Capreomycin