Brief exposure to ethanol augments vascular contractility in human chorionic plate arteries.
Heavy alcohol consumption has been known as a risk factor for hypertension, although the mechanism by which alcohol intake causes hypertension remains elusive. We tested the hypothesis that brief exposure to ethanol augments vascular contractility through the stress response in human chorionic plate arteries. Human chorionic plate arteries were mounted in organ baths and exposed to 5% ethanol for 15, 30 or 45 min. Brief exposure for 45 min, but not 15 min, not only augmented contractility to KCl and 5-hydroxytryptamine 5 h after the end of exposure, but also increased the expression of heat shock protein (HSP) 70 in the tissues. Reverse transcription-polymerase chain reaction showed gradual increases of hsp70 mRNA expression, but not heat shock cognate 70 (hsc70), hsp90alpha or glucose regulatory protein 78 (grp78) mRNA expression, in an exposure time-dependent manner 3 h after the end of exposure. These results indicate that ethanol augments vascular contractility through the stress response.
Hong
Eun Hui
EH
Department of Pharmacology, Kyungpook National University School of Medicine, Daegu, Republic of Korea.
Kang
Na Young
NY
Kim
Jee Ahn
JA
Koo
Tae Bon
TB
Kang
Bong-Seok
BS
Kim
In Kyeom
IK
D001158:Arteries / Q000187:drug effects / Q000502:physiology; D002823:Chorion / Q000098:blood supply* / Q000187:drug effects* / Q000502:physiology; D000091342:Endoplasmic Reticulum Chaperone BiP; D000431:Ethanol / Q000008:administration & dosage*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D066298:In Vitro Techniques; D014661:Vasoconstriction / Q000187:drug effects* / Q000502:physiology
D000091342:Endoplasmic Reticulum Chaperone BiP; D018840:HSP70 Heat-Shock Proteins; C000717247:HSPA5 protein, human; D000431:Ethanol