Isolation and functional assessment of a tomato proteinase inhibitor II gene.
A genomic clone encoding a serine proteinase inhibitor II, designated as TPI-2, was isolated from tomato (Lycopersicon esculentum Mill.) seedling. It consisted of a 990 bp upstream regulatory region and a 680 bp transcription region containing an intron. As shown by northern hybridization, mechanical injury activated its expression in roots, stems and leaves, and so did exogenous hormones jasmonic acid (JA) and alpha-Linolenic acid (LA), though abscisic acid (ABA) and NaCl failed to induce its expression. Salicylic acid (SA) was found to inhibit the inducing effect of LA but not those of mechanical injury and JA. As demonstrated experimentally, TPI-2 could be expressed effectively in tobacco cells and the protein products showed insecticidal activity.
Zhang
Hui-yong
HY
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100080 Beijing, People's Republic of China.
Xie
Xian-zhi
XZ
Xu
Yun-zhe
YZ
Wu
Nai-hu
NH
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000494:pharmacology; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005822:Genetic Vectors; D007438:Introns; D018551:Solanum lycopersicum / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D054883:Oxylipins; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis* / Q000737:chemistry*; D018517:Plant Roots / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics; D011480:Protease Inhibitors / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020156:Salicylic Acid / Q000494:pharmacology; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology; D014026:Nicotiana / Q000235:genetics; D017962:alpha-Linolenic Acid / Q000494:pharmacology
D003517:Cyclopentanes; D018076:DNA, Complementary; D004791:Enzyme Inhibitors; D054883:Oxylipins; D010940:Plant Proteins; D011480:Protease Inhibitors; D015842:Serine Proteinase Inhibitors; D017962:alpha-Linolenic Acid; D012965:Sodium Chloride; C011006:jasmonic acid; D000040:Abscisic Acid; D020156:Salicylic Acid