Development of Abeta terminal end-specific antibodies and sensitive ELISA for Abeta variant.
Alzheimer's disease (AD) is a neurodegenerative affliction associated with memory dysfunction. Senile plaques are a pathological hallmark of AD, and amyloid beta (Abeta) peptides are a major component of these plaques. Abeta peptides are derived from proteolytic cleavage of the Abeta protein precursor (APP) by beta- and gamma-secretases to generate two principal species, Abeta1-40 and Abeta1-42. We have developed antibodies against the N- and C-termini of these peptides, and an ELISA for accurate and sensitive quantitative assessment. Sandwich ELISA composed of N-terminus (Abeta1) end-specific antibody, clone 82E1, and C-termini end-specific antibodies, and clones 1A10 and 1C3 for Abeta40 and Abeta42, respectively, detects full-length Abeta1-40 and 1-42 with a sensitivity in the sub single digit fmol/ml (equivalent to single digit pg/ml) range with no cross-reactivity to APP. A combination of C-termini antibodies and an antibody against the middle region of Abeta detects mouse Abeta in non-transgenic mouse brains.
Horikoshi
Yuko
Y
Immuno-Biological Laboratories Co., Ltd., Fujioka-shi, Gunma 375-0005, Japan.
Sakaguchi
Gaku
G
Becker
Amanda G
AG
Gray
Audrey J
AJ
Duff
Karen
K
Aisen
Paul S
PS
Yamaguchi
Haruyasu
H
Maeda
Masahiro
M
Kinoshita
Noriaki
N
Matsuoka
Yasuji
Y
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000097:blood / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D001921:Brain / Q000166:cytology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism*
D016229:Amyloid beta-Peptides; D000906:Antibodies; D010446:Peptide Fragments