[Distribution of -477C/T single nucleotide polymorphism in the promoter region of ABCA1 gene and its significance for plasma lipids levels in normal Chinese Han population].
OBJECTIVE
To investigate the distribution of -477C/T single nucleotide polymorphisms (SNP) in the promoter region of ATP binding cassette transporter (ABCA1) gene and its relation to plasma lipid levels in normal Chinese Han population.


METHODS
The three genotypes of -477C/T polymorphisms in the promoter region of the ABCA1 gene were detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 113 normal Chinese Han subjects.


RESULTS
-477C/T SNP was present in normal Chinese Han population, and 37.2% (42) of the examined subjects belonged to the genotype of CC, 46.9% (53) belonged to CT and 15.9% (18) to TT. The individuals with the TT genotype had a lower plasma high-density lipoprotein cholesterol (HDL-C) level than those with the CC genotype (P<0.05). No association was found between the genotypes and plasma levels of total cholesterol, triglyceride, low-density lipoprotein, very low-density lipoprotein and body mass index.


CONCLUSIONS
The TT genotype significantly influences the plasma levels of HDL-C in normal Chinese Han individuals.
Liu
Sheng-lin
SL
Department of Cardiology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Guo
Zhi-gang
ZG
Lai
Wen-yan
WY
Tu
Yan
Y
Chen
Jian-ting
JT
D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000328:Adult; D000367:Age Factors; D000368:Aged; D002681:China / Q000208:ethnology; D005260:Female; D006801:Humans; D008055:Lipids / Q000097:blood*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012737:Sex Factors
C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters; D008055:Lipids