Stable carbon isotopic fractionations associated with inorganic carbon fixation by anaerobic ammonium-oxidizing bacteria.
Isotopic analyses of Candidatus "Brocadia anammoxidans," a chemolithoautotrophic bacterium that anaerobically oxidizes ammonium (anammox), show that it strongly fractionates against (13)C; i.e., lipids are depleted by up to 47 per thousand versus CO(2). Similar results were obtained for the anammox bacterium Candidatus "Scalindua sorokinii," which thrives in the anoxic water column of the Black Sea, suggesting that different anammox bacteria use identical carbon fixation pathways, which may be either the Calvin cycle or the acetyl coenzyme A pathway.
Schouten
Stefan
S
Department of Marine Biogeochemistry and Toxicology, Royal Netherlands Institute for Sea Research, 1790 AB Den Burgh, The Netherlands. schouten@nioz.nl
Strous
Marc
M
Kuypers
Marcel M M
MM
Rijpstra
W Irene C
WI
Baas
Marianne
M
Schubert
Carsten J
CJ
Jetten
Mike S M
MS
Sinninghe Damsté
Jaap S
JS
D000693:Anaerobiosis; D001421:Bacteria, Anaerobic / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D019149:Bioreactors; D017554:Carbon Compounds, Inorganic / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes / Q000378:metabolism*; D003470:Culture Media; D050356:Lipid Metabolism; D010084:Oxidation-Reduction; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D012623:Seawater
D017554:Carbon Compounds, Inorganic; D002247:Carbon Isotopes; D003470:Culture Media; D000644:Quaternary Ammonium Compounds; D002245:Carbon Dioxide