Transcriptional control of TFF3 (intestinal trefoil factor) via promoter binding sites for the nuclear factor kappaB and C/EBPbeta.
The acute phase response is strictly connected with modulation of gene expression. Transcriptional control of many genes is mediated by binding of diverse transcription factors to cis-acting DNA motifs in the respective promoter sequence. We now describe such regulatory elements for the TFF3 gene coding for a peptide involved in response to gut irritation. TNF-alpha stimulation, which induces NF-kappaB activation, evoked up to 10-fold reduction of TFF3 expression in the colon tumour cell line HT-29. Several consensus binding sites for members of the NF-kappaB transcription factor subunits are located in the 5'-flanking region of TFF3. Mutating these sites was aimed at discovering which one is responsible for NF-kappaB binding and thus regulation of TFF3 expression.
Baus-Loncar
Mirela
M
Division of Molecular Genetics, Institute of Anthropology & Human Genetics, University of Tübingen, Wilhelmstr 27, 72074, Germany.
Al-azzeh
Ezzal-din
ED
Romanska
Hanna
H
Lalani
El-Nasir
el-N
Stamp
Gordon W H
GW
Blin
Nikolaus
N
Kayademir
Tuncay
T
D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics; D024202:Electrophoretic Mobility Shift Assay; D019073:HT29 Cells; D006801:Humans; D009077:Mucins / Q000235:genetics*; D009124:Muscle Proteins / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D016328:NF-kappa B / Q000235:genetics*; D010455:Peptides; D011401:Promoter Regions, Genetic; D051996:Transcription Factor RelA; D014157:Transcription Factors / Q000235:genetics*; D015533:Transcriptional Activation / Q000187:drug effects*; D000071165:Trefoil Factor-3; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D022762:CCAAT-Enhancer-Binding Proteins; D009077:Mucins; D009124:Muscle Proteins; D016328:NF-kappa B; D010455:Peptides; C082495:TFF3 protein, human; C439524:TNF protein, human; D051996:Transcription Factor RelA; D014157:Transcription Factors; D000071165:Trefoil Factor-3; D014409:Tumor Necrosis Factor-alpha