Lentiviral-mediated gene transfer to model triplet repeat disorders.
This chapter describes the potential use of viral-mediated gene transfer in the central nervous system as a new strategy in developing animal models of neurodegenerative diseases. To illustrate the approach, procedures for the production of lentiviral vectors encoding polyQ proteins are provided, as well as methods for the determination of viral titers, in vitro infection, and basic protocols for in vivo studies in rodents.
Régulier
Etienne
E
Institute of Neuroscience, School of Life Sciences, Swiss Federal Institute of Technology Lausanne, Switzerland.
Zala
Diana
D
Aebischer
Patrick
P
Déglon
Nicole
N
D000818:Animals; D002460:Cell Line; D005260:Female; D018014:Gene Transfer Techniques; D030342:Genetic Diseases, Inborn / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D016086:Lentivirus / Q000235:genetics*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D018911:Trinucleotide Repeats / Q000235:genetics*