Gene transfer of nitric oxide synthase via the use of adeno-associated virus vectors.
Adeno-associated virus (AAV) is a replication-defective dependovirus and is not known to cause disease in humans. AAV vectors have a broad host range and can transduce both dividing and nondividing cells. Five primate AAV serotypes have been characterized in the literature and are designated as AAV types 1-5 (AAV1-5). There is divergence in homology and tropism for various AAV serotypes. AAV vectors can be produced by triple plasmids transfection system (adenovirus-free system) and purified by ultracentrifugation using CsCl gradient. The titer of AAV vectors is determined by dot-blot analysis. We have produced the AAV type 2 vectors encoding endothelial NOS (eNOS) gene and transduced rat aortic segments.
Maeda
Yoshikazu
Y
Division of Cardiovascular Medicine, Jichi Medical School, Tochigi, Japan.
Shimada
Kazuyuki
K
Ikeda
Uichi
U
D000818:Animals; D001011:Aorta / Q000378:metabolism*; D003001:Cloning, Molecular; D000229:Dependovirus / Q000235:genetics*; D018014:Gene Transfer Techniques; D007150:Immunohistochemistry; D019001:Nitric Oxide Synthase / Q000235:genetics* / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III; D051381:Rats
D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat