[Polymorphism and multiplex amplification of 3 X-chromosome specific short tandem repeat loci].
OBJECTIVE
To devise a multiplex PCR system of three X-chromosome specific short tandem repeat (X-STR) loci and study the genetic polymorphism.


METHODS
DXS6799, DXS6804 and DXS6854 were amplified simultaneously using a multiplex system and were typed by polyacrylamide gel electrophoresis and silver staining.


RESULTS
A total of 262 male and 255 female individuals from Guangdong Han population were tested; each locus showed 7 alleles. 73 haplotypes were detected in the male individuals. The haplotype diversity reached 0.9674.


CONCLUSIONS
The 3 X-STR multiplex system is relatively abundant in polymorphic information for forensic identification and paternity testing.
Liu
Qiu-ling
QL
Faculty of Forensic Medicine, Zhongshan Medical College, Sun Yat-sen University, Guangzhou, Guangdong, 510089 PR China. qiulinguser@tom.com
Lü
De-jian
DJ
Cui
Wei
W
D002874:Chromosome Mapping; D041321:Chromosomes, Human, X; D005260:Female; D005784:Gene Amplification; D006239:Haplotypes; D006801:Humans; D008297:Male; D010334:Paternity; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences