title: Enhanced activity and enantioselectivity of Candida rugosa lipase immobilized on macroporous adsorptive resins for ibuprofen resolution.
abstract: The lipase from Candida rugosa was immobilized on three commercially available macroporous adsorptive resins for kinetic resolution of ibuprofen. One resin, CRB02, increased the enzyme activity by 50% to 0.027 g g(-1) min(-1). The deactivation constant (0.19 h(-1)) of the immobilized enzyme was half of that of the native enzyme and the enantioselectivity (E = 29.2) of the immobilized lipase was 2.2 times as much as that of the native lipase for the kinetic resolution of ibuprofen with 1-propanol in isooctane at 30 degrees C.
authors.affiliation: Chemical and Process Engineering Centre, National University of Singapore, 4 Engineering Drive 4, Singapore. cpeyuhw@nus.edu.sg
authors.forename: Hongwei
authors.initials: H
authors.lastname: Yu
authors.forename: Jinchuan
authors.initials: J
authors.lastname: Wu
authors.forename: Chi Bun
authors.initials: CB
authors.lastname: Ching
mesh_terms: D000327:Adsorption; D002175:Candida / Q000737:chemistry / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D007052:Ibuprofen / Q000737:chemistry* / Q000302:isolation & purification; D007700:Kinetics; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism*; D012117:Resins, Synthetic / Q000737:chemistry*; D013237:Stereoisomerism; D015854:Up-Regulation
chemical_list: D004800:Enzymes, Immobilized; D012117:Resins, Synthetic; D008049:Lipase; D007052:Ibuprofen