title: ROS-related enzyme expressions in endothelial cells regulated by tea polyphenols.
abstract: OBJECTIVE
  Elevation of reactive oxygen species (ROS), especially the level of superoxide is a key event in many forms of cardiovascular diseases. To study the mechanism of tea polyphenols against cardiovascular diseases, we observed the expressions of ROS-related enzymes in endothelial cells.
  
  
  METHODS
  Tea polyphenols were co-incubated with bovine carotid artery endothelial cells (BCAECs) in vitro and intracellular NADPH oxidase subunits p22phox and p67phox, SOD-1, and catalase protein were detected using Western blot method.
  
  
  RESULTS
  Tea polyphenols of 0.4 microg/mL and 4.0 microg/mL (from either green tea or black tea) down-regulated NADPH oxidase p22phox and p67phox expressions in a dose-negative manner (P < 0.05), and up-regulated the expressions of catalase (P < 0.05).
  
  
  CONCLUSIONS
  Tea polyphenols regulate the enzymes involved in ROS production and elimination in endothelial cells, and may be beneficial to the prevention of endothelial cell dysfunction and the development of cardiovascular diseases.
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, Hubei, China. yingchenjiang@yahoo.com
authors.forename: Chen-Jiang
authors.initials: CJ
authors.lastname: Ying
authors.forename: Xiu-Fa
authors.initials: XF
authors.lastname: Sun
authors.forename: Shu-Lin
authors.initials: SL
authors.lastname: Zhang
authors.forename: Xi-Ping
authors.initials: XP
authors.lastname: Zhang
authors.forename: Li-Mei
authors.initials: LM
authors.lastname: Mao
authors.forename: Xue-Zhi
authors.initials: XZ
authors.lastname: Zuo
authors.forename: Ping
authors.initials: P
authors.lastname: Yao
mesh_terms: D000818:Animals; D028241:Camellia sinensis / Q000737:chemistry*; D002339:Carotid Arteries / Q000166:cytology; D002374:Catalase / Q000096:biosynthesis; D002417:Cattle; D002478:Cells, Cultured; D015536:Down-Regulation; D042783:Endothelial Cells / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D026901:Membrane Transport Proteins / Q000096:biosynthesis; D009252:NADPH Dehydrogenase / Q000096:biosynthesis; D019255:NADPH Oxidases; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010750:Phosphoproteins / Q000096:biosynthesis; D059808:Polyphenols; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000096:biosynthesis; D000072105:Superoxide Dismutase-1; D015854:Up-Regulation
chemical_list: D005419:Flavonoids; D026901:Membrane Transport Proteins; D010636:Phenols; D010750:Phosphoproteins; D059808:Polyphenols; D017382:Reactive Oxygen Species; C000606290:SOD1 protein, human; C079789:neutrophil cytosol factor 67K; D002374:Catalase; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D019255:NADPH Oxidases; C086986:CYBA protein, human; D009252:NADPH Dehydrogenase