NF-kappaB p50 facilitates neutrophil accumulation during LPS-induced pulmonary inflammation.
BACKGROUND
Transcription factors have distinct functions in regulating immune responses. During Escherichia coli pneumonia, deficiency of NF-kappaB p50 increases gene expression and neutrophil recruitment, suggesting that p50 normally limits these innate immune responses. p50-deficient mice were used to determine how p50 regulates responses to a simpler, non-viable bacterial stimulus in the lungs, E. coli lipopolysaccharide (LPS).


RESULTS
In contrast to previous results with living E. coli, neutrophil accumulation elicited by E. coli LPS in the lungs was decreased by p50 deficiency, to approximately 30% of wild type levels. Heat-killed E. coli induced neutrophil accumulation which was not decreased by p50 deficiency, demonstrating that bacterial growth and metabolism were not responsible for the different responses to bacteria and LPS. p50 deficiency increased the LPS-induced expression of kappaB-regulated genes essential to neutrophil recruitment, including KC, MIP-2, ICAM-1, and TNF-alpha suggesting that p50 normally limited this gene expression and that decreased neutrophil recruitment did not result from insufficient expression of these genes. Neutrophils were responsive to the chemokine KC in the peripheral blood of p50-deficient mice with or without LPS-induced pulmonary inflammation. Interleukin-6 (IL-6), previously demonstrated to decrease LPS-induced neutrophil recruitment in the lungs, was increased by p50 deficiency, but LPS-induced neutrophil recruitment was decreased by p50 deficiency even in IL-6 deficient mice.


CONCLUSIONS
p50 makes essential contributions to neutrophil accumulation elicited by LPS in the lungs. This p50-dependent pathway for neutrophil accumulation can be overcome by bacterial products other than LPS and does not require IL-6.
Mizgerd
Joseph P
JP
Physiology Program, Harvard School of Public Health, Boston, MA, 02115 USA. jmizgerd@hsph.harvard.edu
Lupa
Michal M
MM
Spieker
Matt S
MS
D000818:Animals; D018925:Chemokines / Q000502:physiology; D022361:Escherichia coli Vaccines / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics; D006358:Hot Temperature; D015850:Interleukin-6 / Q000172:deficiency / Q000502:physiology; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D052002:NF-kappa B p50 Subunit; D020556:Neutrophil Infiltration / Q000502:physiology*; D009504:Neutrophils / Q000737:chemistry / Q000502:physiology; D011014:Pneumonia / Q000473:pathology*; D014157:Transcription Factors / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D014613:Vaccines, Attenuated / Q000378:metabolism
D018925:Chemokines; D022361:Escherichia coli Vaccines; D015850:Interleukin-6; D008070:Lipopolysaccharides; D052002:NF-kappa B p50 Subunit; D014157:Transcription Factors; D014613:Vaccines, Attenuated; C073701:Nfkb1 protein, mouse