Cell responses to xenobiotics: comparison of MCF7 multi-drug- and mussel blood cell multi-xenobiotic-defense mechanisms.
Multi-drug resistance (MDR) in MCF7 breast cancer cells and multi-xenobiotic resistance (MXR) in mussel (Mytilus edulis) blood cells (MBC) are well known mechanisms that contribute to the decrease in intracellular concentrations of many unrelated but cytotoxic compounds. In the present work, we have carried out comparative investigations of the MDR/MXR protective mechanisms using a rapid colorimetric assay for cell viability and calcein accumulation for MDR/MXR activities. These studies were performed using cultured MCF7 and MBC before and after in vitro exposure to xenobiotics. Our results indicate that a 5-day exposure to doxorubicin or vincristine decreased calcein accumulation in MBC which is consistent with an induction of multi-xenobiotic resistance. The increase in calcein accumulation provoked by 1-h treatment with 50 microM verapamil was much lower in MBC when compared to the P-glycoprotein overexpressing MCF7 cell line. We conclude that such microplate assays could be used in primary cultures of MBC to estimate the effects of various chemicals on MXR activity.
Marin
Matthieu
M
Laboratory of Ecotoxicology, UPRES-EA 3222, IFRMP 23, University of Le Havre, BP 540 76058 Le Havre, France. matthieu.marin@univ-lehavre.fr
Legros
Hélène
H
Poret
Agnès
A
Leboulenger
François
F
Le Foll
Frank
F
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D049872:Bivalvia / Q000378:metabolism*; D045744:Cell Line, Tumor; D003124:Colorimetry; D004317:Doxorubicin / Q000494:pharmacology; D018432:Drug Resistance, Multiple / Q000187:drug effects*; D005452:Fluoresceins / Q000378:metabolism*; D005470:Fluorometry; D006434:Hemocytes / Q000378:metabolism*; D006801:Humans; D013778:Tetrazolium Salts; D013844:Thiazoles; D014700:Verapamil / Q000494:pharmacology; D014750:Vincristine / Q000494:pharmacology; D015262:Xenobiotics / Q000494:pharmacology
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D005452:Fluoresceins; D013778:Tetrazolium Salts; D013844:Thiazoles; D015262:Xenobiotics; D014750:Vincristine; D004317:Doxorubicin; D014700:Verapamil; C022616:thiazolyl blue; C007740:fluorexon