title: [Change in intestinal function in sepsis in rat].
abstract: OBJECTIVE
  To study the barrier function, absorption, permeability and peristalsis of intestine in sepsis in rats.
  
  
  METHODS
  A Wistar rat model of sepsis was reproduced by ischemia/reperfusion (I/R) of the intestine combined with endotoxin challenge. Animal were randomly divided into normal, I/R 1 hours (I/R 1), I/R 2 hours (I/R 2), I/R 4 hours (I/R 4) and I/RL groups. The following parameters were measured in the experiments: (1) diamin oxidase activity (DAO), D-lactate and D-xylose levels in blood using spectrophotometry; (2) transit function of small intestine; (3) pathological examination of small intestine by light microscope.
  
  
  RESULTS
  The results showed that plasma DAO activity was increased in I/R 1, I/R 4 and I/RL (all P<0.05), and small intestinal tissue DAO was decreased in I/R 2 and I/RL (both P<0.05). Negative correlations were found between plasma and intestinal DAO (r=-0.909, P<0.001). Plasma D-lactate was elevated significantly in I/R 1, I/R 2, and I/RL (all P<0.05). D-xylose content was increased at I/R1 and I/RL groups (both P<0.05), and it was significantly higher than controls at 3 hours. Similarly, a positive correlation was found between plasma DAO activity and plasma D-lactate level (r=0.559, P<0.05).
  
  
  CONCLUSIONS
  The intestinal barrier function, absorption function, permeability, and transit are impaired after gut ischemia/reperfusion combined with endotoxin challenge.
authors.affiliation: Research Department of Burn Institute, 304th Hospital of PLA, Beijing 100037, China.
authors.forename: Jun-You
authors.initials: JY
authors.lastname: Li
authors.forename: Dan
authors.initials: D
authors.lastname: Sun
authors.forename: Yi
authors.initials: Y
authors.lastname: Lu
authors.forename: Hua
authors.initials: H
authors.lastname: Jin
authors.forename: Xiao-Guo
authors.initials: XG
authors.lastname: Jiang
authors.forename: Sen
authors.initials: S
authors.lastname: Hu
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Sheng
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000097:blood; D000818:Animals; D004195:Disease Models, Animal; D007422:Intestines / Q000098:blood supply / Q000502:physiology / Q000503:physiopathology*; D007511:Ischemia / Q000378:metabolism / Q000503:physiopathology; D019344:Lactic Acid / Q000097:blood; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000503:physiopathology*; D013053:Spectrophotometry; D014994:Xylose / Q000097:blood
chemical_list: D019344:Lactic Acid; D014994:Xylose; D006631:Amine Oxidase (Copper-Containing)