Immobilization of lipases on different carriers and their use in synthesis of pentyl isovalerates.
Porcine pancreatic lipase (PPL) and Candida cylindracea lipase (CCL) were immobilized on Celite and Amberlite IRA 938 by deposition from the aqueous solution by the addition of hexane. The influence of the immobilization on the activities of the immobilized lipase derivatives has been studied. The immobilized lipases were used in synthesis of pentyl isovalerates. Various reaction parameters affecting the synthesis of pentyl isovalerates were investigated. The reaction rates were compared with the rates of esterification with free lipases. The immobilized lipases were found to be very effective in the esterification reaction. The lipases immobilized on Celite 545 exhibited better operational stabilities than that of immobilized on Amberlite IRA-938.
Sagiroglu
Ayten
A
Department of Chemistry, Faculty of Sciences and Letter, Trakya University, Edirne, Turkey. atensa@hotmail.com
Telefoncu
Azmi
A
D000818:Animals; D002175:Candida / Q000201:enzymology; D002384:Catalysis; D007692:Diatomaceous Earth / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D004951:Esterification; D004987:Ethers / Q000138:chemical synthesis* / Q000737:chemistry*; D045782:Hemiterpenes; D006586:Hexanes / Q000737:chemistry; D007475:Ion Exchange Resins / Q000737:chemistry; D007668:Kidney / Q000201:enzymology; D008049:Lipase / Q000737:chemistry*; D010421:Pentanoic Acids / Q000737:chemistry*; D000439:Pentanols / Q000737:chemistry*; D013379:Substrate Specificity; D013552:Swine
D004800:Enzymes, Immobilized; D004987:Ethers; D045782:Hemiterpenes; D006586:Hexanes; D007475:Ion Exchange Resins; D010421:Pentanoic Acids; D000439:Pentanols; C008216:isovaleric acid; C488839:pentyl ether; D007692:Diatomaceous Earth; D008049:Lipase