title: Isolation and characterisation of an Indian strain of Mycoplasma mycoides subsp. mycoides type LC from a case of caprine arthritis.
abstract: Isolation and characterisation of an Indian strain of Mycoplasma mycoides subsp. mycoides LC from a case of caprine arthritis is reported in the study. The isolate was identified based on biochemical, digitonin sensitivity and growth inhibition tests. The virulence of the organism was studied by pathogenicity test in mice and goat. The antigenic and genomic profile of the isolate was compared with that of the standard strain (Y-Goat). Using different sets of primers, polymerase chain reaction was employed for rapid detection of the strain.
authors.affiliation: National Referral Laboratory on Mycoplasma, Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, UP, India. vijendra@ivri.up.nic.in
authors.forename: Vijendra P
authors.initials: VP
authors.lastname: Singh
authors.forename: N C
authors.initials: NC
authors.lastname: Srivastava
authors.forename: Manoj
authors.initials: M
authors.lastname: Kumar
authors.forename: M Jai
authors.initials: MJ
authors.lastname: Sunder
authors.forename: J P
authors.initials: JP
authors.lastname: Varshney
mesh_terms: D000818:Animals; D001168:Arthritis / Q000150:complications / Q000276:immunology / Q000662:veterinary*; D001426:Bacterial Proteins / Q000378:metabolism; D015153:Blotting, Western / Q000662:veterinary; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D015511:Goat Diseases / Q000276:immunology / Q000382:microbiology*; D006041:Goats; D007194:India; D051379:Mice; D009176:Mycoplasma mycoides / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000472:pathogenicity; D011002:Pleuropneumonia, Contagious / Q000276:immunology / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes