title: The G-protein-coupled CCK2 receptor associates with phospholipase Cgamma1.
abstract: In ElasCCK2 transgenic mice expressing cholecystokinin (CCK2) receptor in acinar cells, pancreatic phenotypic alterations and preneoplastic lesions are observed. We determined whether activation of phospholipase C gamma1 (PLCgamma1), known to contribute to the tumorigenesis pathophysiology, could take place as a new signaling pathway induced by the CCK2 receptor. Overexpression and activation of the PLCgamma1 in response to gastrin was observed in acinar cells. The possibility that the C-terminal tyrosine 438 of the CCK2 receptor associates with the SH2 domains of PLCgamma1 was examined. A specific interaction was demonstrated using surface plasmon resonance, confirmed in a cellular system and by molecular modeling.
authors.affiliation: INSERM U531, Institut Louis Bugnard, IFR31, CHU Rangueil, Bât L3, Toulouse, France.
authors.forename: Marika
authors.initials: M
authors.lastname: Arnould
authors.forename: Amina
authors.initials: A
authors.lastname: Tassa
authors.forename: Audrey
authors.initials: A
authors.lastname: Ferrand
authors.forename: Elodie
authors.initials: E
authors.lastname: Archer
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Estève
authors.forename: Virginie
authors.initials: V
authors.lastname: Pénalba
authors.forename: Ghislaine
authors.initials: G
authors.lastname: Portolan
authors.forename: Achim
authors.initials: A
authors.lastname: Escherich
authors.forename: Luis
authors.initials: L
authors.lastname: Moroder
authors.forename: Daniel
authors.initials: D
authors.lastname: Fourmy
authors.forename: Catherine
authors.initials: C
authors.lastname: Seva
authors.forename: Marlène
authors.initials: M
authors.lastname: Dufresne
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004789:Enzyme Activation; D007150:Immunohistochemistry; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D051966:Phospholipase C gamma; D011485:Protein Binding; D043887:Receptor, Cholecystokinin B / Q000737:chemistry / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D010738:Type C Phospholipases / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism
chemical_list: D043887:Receptor, Cholecystokinin B; D014443:Tyrosine; D010738:Type C Phospholipases; D051966:Phospholipase C gamma