Microsomal estrogen metabolism in channel catfish.
Our goal was to study the involvement of cytochrome P450 genes in estrogen metabolism and the extent to which the potentially carcinogenic 4-hydroxyestradiol metabolite is formed by channel catfish (Ictalurus punctatus; CC). Estradiol metabolism and ethoxyresorufin-O-deethylase (EROD) activity were assessed in several tissues from fish collected from three variably contaminated sites in the Mississippi River Delta, from laboratory control fish, and from fish exposed to 20 mg/kg benzo(a)pyrene (BaP) i.p. for 4 days. Liver EROD activity was induced by BaP, but Delta fish EROD activities were not statistically higher than activities in control fish. Gill microsomal EROD activity was also induced by BaP, but activities were 8- to 77-fold lower than those from liver. The predominant estrogen metabolites formed by CC liver, gill and gonad microsomes were 2-hydroxyestradiol and estrone as detected by GC/MS. Liver and gill 2-hydroxyestradiol formation was induced in BaP-dosed fish. The trends in hydroxyestradiol formation in field collected fish were more variable. In all fish liver microsomes there was more 2- compared to 4-hydroxyestradiol formed. However, BaP-treatment increased the 4:2 hydroxyestradiol ratio from 0.04 in control fish to 0.2 in BaP-exposed fish, suggesting that BaP induces the formation of the potentially genotoxic estrogen metabolite. No detectable 4-hydroxyestradiol was produced by gill and gonad microsomes. These results will ultimately help in determining which fish P450 genes are susceptible to environmental contamination and may be involved in estrogen genotoxicity.
Butala
Harshala
H
Department of Chemical Engineering, University of Mississippi, University, MS 38677-1848, USA.
Metzger
Christine
C
Rimoldi
John
J
Willett
Kristine L
KL
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002397:Catfishes / Q000378:metabolism*; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis*; D004790:Enzyme Induction / Q000187:drug effects; D004958:Estradiol / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism*; D004970:Estrone / Q000096:biosynthesis; D005618:Fresh Water; D008401:Gas Chromatography-Mass Spectrometry; D005880:Gills / Q000378:metabolism; D006066:Gonads / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism*; D008922:Mississippi
D004970:Estrone; D001564:Benzo(a)pyrene; D004958:Estradiol; C001390:2-hydroxyestradiol; D019363:Cytochrome P-450 CYP1A1