Polyethylene glycol-grafted polystyrene particles.
Densely pegylated particles that can serve as a model system for artificial cells were prepared by covalently grafting amino polyethylene glycol (PEG, molecular weight 3400 or 5000) onto carboxyl polystyrene particles (PS-COOH) using carbodiimide chemistry. PEG-modified particles (PS-PEG) were characterized by determination of the PEG surface concentration, zeta-potential, size, and morphology. Under optimized grafting conditions, a dense "brush-like" PEG layer was formed. A PEG surface concentration of approximately 60 pmol/cm2, corresponding with an average distance between grafted PEG chains of approximately 17 A can be realized. It was shown that grafting of PEG onto PS-COOH reduced the adsorption of proteins from human plasma (85 vol %) in phosphate-buffered saline up to 90%.
Meng
Fenghua
F
Polymer Chemistry and Biomaterials Group, Department of Chemical Technology, Institute for Biomedical Technology (BMTI), University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
Engbers
Gerard H M
GH
Feijen
Jan
J
D000327:Adsorption; D000588:Amines / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D001798:Blood Proteins / Q000737:chemistry; D004553:Electric Conductivity; D004563:Electrochemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D010316:Particle Size; D010949:Plasma / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D013499:Surface Properties; D014302:Trinitrobenzenesulfonic Acid / Q000737:chemistry
D000588:Amines; D001672:Biocompatible Materials; D001798:Blood Proteins; D007202:Indicators and Reagents; D011137:Polystyrenes; D011092:Polyethylene Glycols; D014302:Trinitrobenzenesulfonic Acid