title: Analysis of the sinusitis nasal lavage fluid proteome using capillary liquid chromatography interfaced to electrospray ionization-quadrupole time of flight- tandem mass spectrometry.
abstract: The nasal lavage fluids (NLFs) from four subjects with acute sinusitis were analyzed to investigate the amount of proteins expressed in this pathology at the beginning of the event (day 1) and after 6 days of treatment with antibiotics and a nasal steroid spray. The protein identification was performed with capillary liquid chromatography-electrospray-quadrupole time of flight-(LC-ESI-Q-TOF)-mass spectrometry. The samples collected on the first day contained high-abundant plasma proteins, such as albumin and immunoglobulins, glandular serous cell proteins (lysozyme, lactoferrin, and polymeric immunoglobulin receptor), epithelial keratins, and inflammatory cell proteins (myeloperoxidase, IL-16, and IL-17E). After six days of therapy, the complexity of the proteome was reduced to plasma proteins and lysozyme with no inflammatory markers. The presence of hemoglobin, however, suggested that significant squamous metaplasia with breaches in the epithelial barrier, or nasal steroid-related bleeding, had occurred. The proteomic approach presented here allowed us to identify, in the high complexity of acute sinusitis nasal secretions, the proteins that respond to a pharmacological treatment and that could be suitable as markers of this pathology.
authors.affiliation: Division of Rheumatology, Georgetown University, Washington, DC, USA. bc48@georgetown.edu
authors.forename: Begona
authors.initials: B
authors.lastname: Casado
authors.forename: Lewis K
authors.initials: LK
authors.lastname: Pannell
authors.forename: Simona
authors.initials: S
authors.lastname: Viglio
authors.forename: Paolo
authors.initials: P
authors.lastname: Iadarola
authors.forename: James N
authors.initials: JN
authors.lastname: Baraniuk
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001798:Blood Proteins / Q000378:metabolism*; D002853:Chromatography, Liquid; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D019410:Interleukin-16 / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009113:Muramidase / Q000378:metabolism*; D017817:Nasal Lavage Fluid / Q000737:chemistry*; D009195:Peroxidase / Q000378:metabolism; D020543:Proteome; D012852:Sinusitis / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000900:Anti-Bacterial Agents; D001798:Blood Proteins; D016207:Cytokines; D006454:Hemoglobins; D019410:Interleukin-16; D020381:Interleukin-17; D020543:Proteome; D009195:Peroxidase; D009113:Muramidase