title: [The effect of CTLA4Ig-modified dendritic cells on proliferation and cytotoxicity of lymphocytes in-vitro].
abstract: OBJECTIVE
  To study the effect of dendritic cells (DCs) modified by CTLA4Ig on T-cell proliferation and the cytotoxic T lymphocyte killer activity.
  
  
  METHODS
  The expressing vector pG/CTLA4Ig was transfected into DCs via lipofectamine reagent mediation. CTLA4Ig fusion protein secreted by DCs in the culture supernatant was confirmed by sandwich ELISA and SDS-PAGE. Peripheral blood mononuclear cells (PBMC) from C57BL/6 mice (as reaction cells) and DCs modified or unmodified by CTLA4Ig (as stimulation cells) were co-cultured for 6 days. MTT colorimetry was used to detect lymphocyte proliferation. Lactate dehydrogenase release method and sandwich ELISA assay was used to examine the cytotoxic activity and T-cell apoptosis.
  
  
  RESULTS
  CTLA4Ig fusion protein and DCs modified by CTLA4Ig could significantly inhibit lymphocyte proliferation response and cytotoxic activity of specific cytotoxic T lymphocytes, and induce T-cell apoptosis, while unmodified-DCs could markedly induce lymphocyte proliferation response.
  
  
  CONCLUSIONS
  The DCs of stably expressing CTLA4Ig fusion protein could not only markedly inhibit T-cell proliferation and cytotoxic activity of CTL, but also induce T-cell apoptosis.
authors.affiliation: Department of Immunology, Tongji Medical College,Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Wen-hong
authors.initials: WH
authors.lastname: Qiu
authors.forename: Kai-wen
authors.initials: KW
authors.lastname: Guo
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jin
authors.initials: J
authors.lastname: Yang
authors.forename: Guo-hua
authors.initials: GH
authors.lastname: Wang
authors.forename: Wen-jun
authors.initials: WJ
authors.lastname: Liao
authors.forename: Guan-xin
authors.initials: GX
authors.lastname: Shen
mesh_terms: D000069594:Abatacept; D000818:Animals; D017209:Apoptosis; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000502:physiology*; D018796:Immunoconjugates / Q000494:pharmacology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008810:Mice, Inbred C57BL; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014162:Transfection
chemical_list: D018796:Immunoconjugates; D000069594:Abatacept