Novel splicing variant of the human orphan nuclear receptor Nurr1 gene.
BACKGROUND
Nurr1 is a member of the nuclear receptor superfamily of transcription factors. The objective of the present study was to identify novel splicing variants of the gene in neuronal and non-neuronal tissues and determine their functions.


METHODS
Reverse transcription-polymerase chain reaction (RT-PCR) analysis was used to screen for Nurr1 splice variants in the adult human central nervous system (CNS) and in other tissues such as lymphocytes, and liver, muscle, and kidney cells. Functional assays of the variants were performed by measuring Nurr1 response element (NuRE) transcriptional activity in vitro.


RESULTS
In this study, the authors identified a novel splicing variant of Nurr1 within exon 5, found in multiple adult human tissues, including lymphocytes, and liver, muscle, and kidney cells, but not in the brain or spinal cord. Sequencing analysis showed the variant has a 75 bp deletion between nucleotides 1402 and 1476. A functional assay of the Nurr1-c splicing variant, performed by measuring NuRE transcriptional activity in vitro, detected a 39% lower level of luciferase (LUC) activity (P < 0.05).


CONCLUSIONS
A novel splicing variant of Nurr1 exists in human non-neuronal tissues and functional assays suggest that the variant may act as an alternate transcription regulator.
Xu
Ping-yi
PY
Department of Neurology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
Le
Wei-dong
WD
D000328:Adult; D017398:Alternative Splicing; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics*; D006801:Humans; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics*
D004268:DNA-Binding Proteins; C539882:NR4A2 protein, human; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2; D014157:Transcription Factors