title: Methionine adenosyltransferase alpha-helix structure unfolds at lower temperatures than beta-sheet: a 2D-IR study.
abstract: Two-dimensional infrared spectroscopy has been used to characterize rat liver methionine adenosyltransferase and the events taking place during its thermal unfolding. Secondary structure data have been obtained for the native recombinant enzyme by fitting the amide I band of infrared spectra. Thermal denaturation studies allow the identification of events associated with individual secondary-structure elements during temperature-induced unfolding. They are correlated to the changes observed in enzyme activity and intrinsic fluorescence. In all cases, thermal denaturation proved to be an irreversible process, with a T(m) of 47-51 degrees C. Thermal profiles and two-dimensional infrared spectroscopy show that unfolding starts with alpha-helical segments and turns, located in the outer part of the protein, whereas extended structure, associated with subunit contacts, unfolds at higher temperatures. The data indicate a good correlation between the denaturation profiles obtained from activity measurements, fluorescence spectroscopy, and the behavior of the infrared bands. A study of the sequence of events that takes place is discussed in light of the previous knowledge on methionine adenosyltransferase structure and oligomerization pathway.
authors.affiliation: Unidad de Biofisica (Centro Mixto CSIC-UPV) and Departamento de Bioquimica, Universidad del Pais Vasco, Bilbao, Spain.
authors.forename: Ibon
authors.initials: I
authors.lastname: Iloro
authors.forename: Rosana
authors.initials: R
authors.lastname: Chehín
authors.forename: Félix M
authors.initials: FM
authors.lastname: Goñi
authors.forename: María A
authors.initials: MA
authors.lastname: Pajares
authors.forename: José-Luis R
authors.initials: JL
authors.lastname: Arrondo
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000235:genetics; D008716:Methionine Adenosyltransferase / Q000737:chemistry*; D017433:Protein Structure, Secondary / Q000502:physiology; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry*; D013055:Spectrophotometry, Infrared / Q000379:methods*; D013696:Temperature
chemical_list: D011994:Recombinant Proteins; D008716:Methionine Adenosyltransferase