Formation of phosphatidic acid, ceramide, and diglyceride on radiolysis of lipids: identification by MALDI-TOF mass spectrometry.
By use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, phosphatidic acid was found to be the main product of gamma radiolysis of cardiolipin, phosphatidylinositol, and phosphatidylglycerol. It has been shown that gamma irradiation of such glycolipids as cerebroside and galactosyl diglyceride leads to formation of ceramide and diglyceride, respectively. These findings, combined with those obtained earlier, allowed an assumption to be made that, owing to radiation-induced free radical fragmentation of lipids in their polar moiety, formation of signaling molecules can occur.
Shadyro
Oleg
O
Department of Chemistry, Belarusian State University, Minsk, Belarus. shadyro@open.by
Yurkova
Irina
I
Kisel
Mikhail
M
Brede
Ortwin
O
Arnhold
Jürgen
J
D000818:Animals; D002308:Cardiolipins / Q000737:chemistry; D002518:Ceramides / Q000737:chemistry* / Q000378:metabolism; D002645:Chickens; D002855:Chromatography, Thin Layer; D053938:DNA Fragmentation; D004075:Diglycerides / Q000737:chemistry*; D004307:Dose-Response Relationship, Radiation; D005609:Free Radicals; D038983:Galactolipids / Q000737:chemistry; D007477:Ions; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000378:metabolism; D008956:Models, Chemical; D010712:Phosphatidic Acids / Q000737:chemistry*; D010715:Phosphatidylglycerols / Q000737:chemistry; D010716:Phosphatidylinositols / Q000737:chemistry; D015398:Signal Transduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002308:Cardiolipins; D002518:Ceramides; D004075:Diglycerides; D005609:Free Radicals; D038983:Galactolipids; D007477:Ions; D008055:Lipids; D008081:Liposomes; D010712:Phosphatidic Acids; D010715:Phosphatidylglycerols; D010716:Phosphatidylinositols; C475298:diacylgalactosylglycerol