WT1 gene expression: useful marker for minimal residual disease in childhood myelodysplastic syndromes and juvenile myelo-monocytic leukemia?
The WT1 gene is considered to be highly expressed in patients with acute myeloid leukemia (AML), acute lymphoblastic leukemia and chronic myeloid leukemia and is thought to play a key role in maintaining the viability of leukemia cells. However, little is known about the WT1 gene expression levels in pediatric patients with juvenile myelo-monocytic leukemia (JMML) and myelodysplastic syndromes (MDS). We studied WT1 expression in diagnostic bone marrow (BM) and peripheral blood (PB) samples of 90 patients with JMML, low grade MDS, advanced MDS and myelodysplasia-related AML in BM (n = 20) and PB (n = 18) samples of normal healthy volunteer donors.
Bader
Peter
P
University Children's Hospital Tübingen, Germany. peter.bader@med.uni-tuebingen.de
Niemeyer
Charlotte
C
Weber
Gerrit
G
Coliva
Tiziana
T
Rossi
Vincenzo
V
Kreyenberg
Hermann
H
Gerecke
Anja
A
Biondi
Andrea
A
D000754:Anemia, Refractory, with Excess of Blasts / Q000175:diagnosis; D014408:Biomarkers, Tumor / Q000032:analysis*; D001853:Bone Marrow / Q000737:chemistry; D002648:Child; D015870:Gene Expression; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis; D015479:Leukemia, Myelomonocytic, Acute / Q000175:diagnosis*; D009190:Myelodysplastic Syndromes / Q000175:diagnosis*; D018365:Neoplasm, Residual; D012333:RNA, Messenger / Q000032:analysis / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025721:WT1 Proteins / Q000235:genetics*
D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D025721:WT1 Proteins