[Constructing the tumor cell vaccine based on homologous matrix metalloproteinase-2 and exploring its effects].
OBJECTIVE
To construct the tumor cell vaccine based on homologous matrix metalloproteinase-2 (MMP-2), and explore its anti-tumor effects.


METHODS
The tumor cell vaccine was constructed by transfecting chicken MMP-2 to tumor cells. MTT colorimetric assay was used to evaluate the activation of the transfectants. Then we observed the formative tumor response in vivo and evaluated the effects of the tumour cell vaccine in immunized mice rechallenged with parental cells.


RESULTS
Using RT-PCR, we picked out the cell strain with the highest expression level from the transfectants. The MTT colorimetric assay revealed that the transfectants increased the expression of the MMP-2 of tumor cells while they did not change the trend of tumor cells' growth. And in animal experiments the vaccine was preliminarily found to be effective for inhibiting the tumour growth and prolonging the survival time of animals.


CONCLUSIONS
We have constructed the tumor cell vaccine based on homologous matrix metalloproteinase-2. The experiments demonstrated that breaking the immune tolerance of MMP-2 by means of active immunity should be a useful approach to cancer therapy.
Yi
Tao
T
Key Laboratory of Biotherapy of Human Diseases of Ministry of Education, Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China.
Tian
Ling
L
Su
Jing-mei
JM
Liu
Jian
J
Wei
Yu-quan
YQ
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000818:Animals; D019496:Cancer Vaccines / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D002645:Chickens; D003110:Colonic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014162:Transfection; D014407:Tumor Cells, Cultured
D019496:Cancer Vaccines; D020778:Matrix Metalloproteinase 2