Kinetic characters and resistance to inhibition of crude and purified brain acetylcholinesterase of three freshwater fishes by organophosphates.
Acetylcholinesterase (AChE) was purified from the brain of three fresh-water fishes, topmouth gudgeon (Pseudorasbora parva), goldfish (Carassius auratus auratus) and rainbow trout (Oncorrhychus mykiss, formerly named Salmo gairdneri) by PEG2000/phosphate-salt two phases extraction, DEAE-Sephadex A-50 and Sephadex G-200 chromatography. Kinetic characters and resistance to inhibition of crude and purified enzymes by organophosphates were then studied. Although the crude enzyme from the trout displayed a different specific activity, kinetic curve, Vmax, and sensitivity to inhibition by oxidized malathion and triazopos compared with the two cyprinoids (i.e. topmouth gudgeon and goldfish), the purified enzymes of all the three species showed no significant difference in all aspects. The result suggested a negligible intrinsic difference of brain AChEs among the tested species.
Shaonan
Li
L
Institute of Pesticides and Environmental Toxicology, College of Agriculture and Biotechnology, Zhejiang University, Zhejiang Hangzhou 310029, China. snli@zju.edu.cn
Xianchuan
Xie
X
Guonian
Zhu
Z
Yajun
Tan
T
D000110:Acetylcholinesterase / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D005399:Fishes / Q000378:metabolism*; D005618:Fresh Water; D007306:Insecticides / Q000633:toxicity*; D007700:Kinetics; D008294:Malathion / Q000633:toxicity; D063086:Organothiophosphates / Q000633:toxicity; D014230:Triazoles / Q000633:toxicity
D007306:Insecticides; D063086:Organothiophosphates; D014230:Triazoles; C012584:triazophos; D000110:Acetylcholinesterase; D008294:Malathion