title: [Cloning and eukaryotic expression of mouse interleukin 21].
abstract: OBJECTIVE
  To clone mouse interleukin 21(mIL-21) gene and construct its eukaryotic expression vector.
  
  
  METHODS
  mIL-21 cDNA was amplified from ConA-activated mouse T cells by RT-PCR. And then the cDNA was cloned into eukaryotic expression plasmid pcDNA3.1 to construct recombinant plasmid pcDNA3.1/mIL-21 which was introduced into Sp2/0 cells by lipofectin. Expression of mIL-21 gene was detected by RT-PCR and NK cytotoxicity assay.
  
  
  RESULTS
  Recombinant plasmid pcDNA3.1/mIL-21 was constructed correctly and mIL-21 gene was expressed in transfected Sp2/0 cells.
  
  
  CONCLUSIONS
  mIL-21 gene was cloned and expressed successfully, which lays the foundation for further studying anti-tumor effect of mIL-21 gene therapy in animal models.
authors.affiliation: Department of Microbiology and Immunology, Medical College, Southeast University, Nanjing 210009, China. pawnking@vip.sina.com
authors.forename: Guo-bing
authors.initials: GB
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Dou
authors.forename: Jun-song
authors.initials: JS
authors.lastname: Chen
authors.forename: Xiao-wu
authors.initials: XW
authors.lastname: Hong
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D003602:Cytotoxicity, Immunologic; D005260:Female; D015316:Genetic Therapy; D007378:Interleukins / Q000235:genetics*; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000378:metabolism; D014162:Transfection; D000098546:Interleukin-21
chemical_list: D007378:Interleukins; D000098546:Interleukin-21