title: [Effects of tea polyphenols and tea pigments on telomerase activity of HepG2 cells].
abstract: OBJECTIVE
  This study is to investigate the effect of tea polyphenols and tea pigments on telomerase activity of human liver cancer cell line, HepG2 cells.
  
  
  METHODS
  TRAP-PCR-ELISA was applied to investigate the telomerase activity.
  
  
  RESULTS
  Telomerase was positive in tea polyphenols treated groups, tea pigments treated groups and blank control group. Telomerase activities (A(450 approximately 690) values) were 1.56 and 1.46 in 50 mg/L and 100 mg/L tea polyphenols-treated groups, 1.55 and 1.49 in 50 mg/L and 100 mg/L tea pigments-treated groups, respectively. The results showed that telomerase activity was significantly inhibited by tea polyphenols and tea pigments treatment as compared with the blank control group (A(450 approximately 690) = 2.11).
  
  
  CONCLUSIONS
  Tea polyphenols and tea pigments could significantly inhibit telomerase activity of HepG2 cells, and telomerase activity may be a useful biomarker for cancer chemoprevention.
authors.affiliation: Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Xu-dong
authors.initials: XD
authors.lastname: Jia
authors.forename: Chi
authors.initials: C
authors.lastname: Han
authors.forename: Jun-shi
authors.initials: JS
authors.lastname: Chen
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000473:pathology; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology / Q000473:pathology; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010860:Pigments, Biological / Q000302:isolation & purification / Q000494:pharmacology*; D059808:Polyphenols; D013662:Tea / Q000737:chemistry*; D019098:Telomerase / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D005419:Flavonoids; D010636:Phenols; D010860:Pigments, Biological; D059808:Polyphenols; D013662:Tea; D019098:Telomerase