title: Identification of the heat shock protein 60 epitope involved in receptor binding on macrophages.
abstract: In the present study, we identified the human heat shock protein 60 (HSP60) epitope responsible for binding to macrophages. Studies using overlapping 15- and 20-mer peptides of the human HSP60 sequence to compete with binding of HSP60 to macrophages indicated that surface binding was accounted for by the region aa481-500. Deletion mutants of HSP60, lacking the N-terminal 137, 243 or 359 amino acids, strongly inhibited HSP60 binding to macrophages. Monoclonal antibodies addressing regions aa1-200, aa335-366 or aa383-447 did not block HSP60 binding. We conclude that a single C-terminal region, aa481-500, accounts for the binding of HSP60 to macrophages.
authors.affiliation: German Diabetes Research Institute, Leibniz Institute at the Heinrich-Heine-University of Düsseldorf, D-40225 Düsseldorf, Germany. christiane.habich@ddfi.uni-duesseldorf.de
authors.forename: Christiane
authors.initials: C
authors.lastname: Habich
authors.forename: Karina
authors.initials: K
authors.lastname: Kempe
authors.forename: Volker
authors.initials: V
authors.lastname: Burkart
authors.forename: Ruurd
authors.initials: R
authors.lastname: Van Der Zee
authors.forename: Mark
authors.initials: M
authors.lastname: Lillicrap
authors.forename: Hill
authors.initials: H
authors.lastname: Gaston
authors.forename: Hubert
authors.initials: H
authors.lastname: Kolb
mesh_terms: D000818:Animals; D002460:Cell Line; D018834:Chaperonin 60 / Q000276:immunology* / Q000378:metabolism; D000939:Epitopes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D018834:Chaperonin 60; D000939:Epitopes; D011956:Receptors, Cell Surface