[Development of an antigen for the diagnosis of Kilham rat parvovirus by hemagglutination inhibition test].
An antigen of rat parvovirus (Kilham virus) was developed for the diagnosis of viral infection in rat colonies by using hemagglutination inhibition (HAI) test. Primary cell cultures from rat embryos were infected with Kilham rat virus. Infected cells obtained at different time post infection were scraped, centrifuged, concentrated one hundred times, sonicated and centrifuged again. The supernatants obtained were titrated by hemagglutination. The specificity was confirmed with positive and negative reference sera. Ninety eight serum samples were studied by using HAI test. The results coincided with those obtained in a reference laboratory. Kilham rat parvovirus antigen obtained from 5 days-infected-cells was specific, sensitive, easy to prepare, with a high yield and it is useful to detect this virus in experimental and production rat colonies.
Ayala
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MA
Cátedra de Animales de Laboratorio,, Facultad de Ciencias Veterinarias, UNLP, Calle 60 y 118, 1900 La Plata, Argentina.
Laborde
J
J
Milocco
S
S
Carbone
C
C
Cid de la Paz
V
V
Galosi
C M
CM
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000032:analysis*; D002478:Cells, Cultured / Q000821:virology; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary*; D007756:Laboratory Animal Science / Q000379:methods; D010322:Parvoviridae Infections / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D017992:Parvovirus / Q000276:immunology* / Q000302:isolation & purification; D051381:Rats / Q000196:embryology / Q000821:virology*; D011921:Rats, Inbred WKY; D012376:Rodent Diseases / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D013047:Specific Pathogen-Free Organisms; D014776:Virus Cultivation
D000914:Antibodies, Viral; D000956:Antigens, Viral