title: Rat adult stem cells (marrow stromal cells) engraft and differentiate in chick embryos without evidence of cell fusion.
abstract: Cell fusion was recently reported to account for the plasticity of adult stem cells in vivo. Adult stem cells, referred to as mesenchymal stem cells or marrow stromal cells, from rat marrow, were infused into 1.5- to 2-day-old chick embryos. After 4 days, the rat cells had expanded 1.3- to 33-fold in one-third of surviving embryos. The cells engrafted into many tissues, and no multinuclear cells were detected. The most common site of engraftment was the heart, apparently because the cells were infused just above the dorsal aorta. Some of the cells in the heart expressed cardiotin, and alpha-heavy-chain myosin. GFP(+) cells reisolated from the embryos had a rat karyotype. Therefore, the cells engrafted and partially differentiated without evidence of cell fusion.
authors.affiliation: Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA 70112, USA.
authors.forename: Radhika R
authors.initials: RR
authors.lastname: Pochampally
authors.forename: Brian T
authors.initials: BT
authors.lastname: Neville
authors.forename: Emily J
authors.initials: EJ
authors.lastname: Schwarz
authors.forename: Marilyn M
authors.initials: MM
authors.lastname: Li
authors.forename: Darwin J
authors.initials: DJ
authors.lastname: Prockop
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002459:Cell Fusion; D002642:Chick Embryo / Q000166:cytology* / Q000502:physiology; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D007621:Karyotyping; D008164:Luminescent Proteins / Q000032:analysis; D051381:Rats; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D014183:Transplantation, Heterologous
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins