A molecular description of brain trauma pathophysiology using microarray technology: an overview.
It has been estimated that 50% of human transcriptome, the collection of mRNA in a cell, is expressed in the brain, making it one of the most complex organs to understand in terms of genomic responses to injury. The availability of genome sequences for several organisms coupled with the increasing affordability of microarray technologies makes it feasible to monitor the mRNA levels of thousands of genes simultaneously. In this paper, we provide an overview of findings using both cDNA- and oligonucleotide-based microarray analyses after experimental traumatic brain injury (TBI). Specifically, the utility of this methodology as a means of cataloging the biochemical sequelae of brain trauma and elucidating novel genes or pathways for further study is discussed. Furthermore, we offer future directions for the continued evaluation of microarray results and discuss the usefulness of microarray techniques as a testing format for determining the efficacy of mechanism-based therapies.
Dash
Pramod K
PK
Department of Neurobiology and Anatomy, and The Vivian L. Smith Center for Neurologic Research, The University of Texas Medical School, Houston, Texas 77225, USA. p.dash@uth.tmc.edu
Kobori
Nobuhide
N
Moore
Anthony N
AN
D000818:Animals; D001921:Brain / Q000473:pathology / Q000503:physiopathology*; D001930:Brain Injuries / Q000235:genetics* / Q000503:physiopathology*; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D009838:Oligodeoxyribonucleotides; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015203:Reproducibility of Results; D014158:Transcription, Genetic / Q000235:genetics
D018076:DNA, Complementary; D009838:Oligodeoxyribonucleotides