title: Somatic DNA recombination yielding circular DNA and deletion of a genomic region in embryonic brain.
abstract: In this study, a mouse genomic region is identified that undergoes DNA rearrangement and yields circular DNA in brain during embryogenesis. External region-directed inverse polymerase chain reaction on circular DNA extracted from late embryonic brain tissue repeatedly detected DNA of this region containing recombination joints. Wide-range genomic PCR and digestion-circularization PCR analysis showed this region underwent recombination accompanied with deletion of intervening sequences, including the circularized regions. This region was mapped by fluorescence in situ hybridization to C1 on mouse chromosome 16, where no gene and no physiological DNA rearrangement had been identified. DNA sequence in the region has segmental homology to an orthologous region on human chromosome 3q.13. These observations demonstrated somatic DNA recombination yielding genomic deletions in brain during embryogenesis.
authors.affiliation: Department of Molecular Genetics, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Higashi-ku, Maidashi, Fukuoka 812-8582, Japan. maedat@beppu.kyushu-u.ac.jp
authors.forename: Toyoki
authors.initials: T
authors.lastname: Maeda
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Chijiiwa
authors.forename: Hideo
authors.initials: H
authors.lastname: Tsuji
authors.forename: Saburo
authors.initials: S
authors.lastname: Sakoda
authors.forename: Kenzaburo
authors.initials: K
authors.lastname: Tani
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000502:physiology*; D002875:Chromosomes / Q000235:genetics / Q000378:metabolism; D004270:DNA, Circular / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D004622:Embryo, Mammalian / Q000033:anatomy & histology / Q000502:physiology*; D017353:Gene Deletion; D015321:Gene Rearrangement; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011995:Recombination, Genetic
chemical_list: D004270:DNA, Circular