[Analysis of the change in clonotypes of T cells accumulated in 4 feet joints of SKG mice].
OBJECTIVE
To understand the relationship between T cell clonotypes and RA invasion through clonotypes of analysis of T cells accumulated in 4 feet joints of SKG mice.


METHODS
Comparative analysis of T cells clonotypes of accumulated in the joints of SKG mice, a spontaneous RA mouse model, was carried out by RT-PCR/SSCP at the initial and late-stages of RA.


RESULTS
The identical rate of Vbeta2 and Vbeta8.2 T cell clonotypes in 4 feet joints increased significantly at the late stage of RA (72.3% and 60.2%, respectively).


CONCLUSIONS
The T cells with Vbeta2 or Vbeta8.2 TCR may play an important role in the RA development of SKG mice.
Zhao
Wen-ming
WM
Department of Immunology, Capital University of Medical Sciences, Beijing 100054, China. zhao-wenming@163.com
Yamamoto
Kazuhiko
K
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D005260:Female; D033023:Foot Joints / Q000276:immunology*; D019674:Genes, T-Cell Receptor beta; D008297:Male; D051379:Mice; D018807:Polymorphism, Single-Stranded Conformational; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology*