Hyphae formation of Candida albicans is regulated by polyamines.
Candida albicans generally grows in hyphae form in RPMI1640 medium. However, addition of 1,4-diamino-2-butanone (DAB), a competitive inhibitor of ornithine decarboxylase, decreased the amount of polyamines in C. albicans, and induced the proliferation of the C. albicans yeast form. The expression of CYR1 mRNA was significantly inhibited by the addition of DAB compared with that of the control. The amount of intracellular cAMP was also decreased by the addition of DAB. A specific adenylate cyclase inhibitor, cis-N-[2-phenylcyclopentyl]-azacyclotridec-1-en-2-amine (MDL-12,330A) promoted the growth of the yeast form. These results indicated that polyamines exist upstream of the adenylate cyclase-cAMP signal pathway and regulate the transformation of C. albicans.
Ueno
Yukihiro
Y
Department of Microbiology, Tohoku Pharmaceutical University, Sendai, Japan.
Fukumatsu
Makoto
M
Ogasawara
Ayako
A
Watanabe
Toshihiko
T
Mikami
Takeshi
T
Matsumoto
Tatsuzi
T
D002176:Candida albicans / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D025301:Hyphae / Q000187:drug effects / Q000378:metabolism*; D011073:Polyamines / Q000378:metabolism* / Q000494:pharmacology; D011700:Putrescine / Q000031:analogs & derivatives* / Q000494:pharmacology
D011073:Polyamines; C014458:1,4-diaminobutanone; D011700:Putrescine