title: The interaction of F4 fimbriae with porcine enterocytes as analysed by surface plasmon resonance.
abstract: Fimbriae often play a prominent role in anchoring bacterial cells to host tissue and mediate the first step in pathogenesis. As a consequence, there is a continuous development of new strategies to block the binding of fimbriae to their specific receptor on host cells. The present study demonstrates the specific interaction of F4 (K88) fimbriae and porcine enterocytes using a real-time biomolecular interaction analysis system (BIAcore 3000), based on the principles of surface plasmon resonance (SPR). This method offers new opportunities to screen therapeutics for prevention of adhesion and subsequent disease without receptor purification.
authors.affiliation: Laboratory of Veterinary Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. frank.verdonck@ugent.be
authors.forename: Frank
authors.initials: F
authors.lastname: Verdonck
authors.forename: Eric
authors.initials: E
authors.lastname: Cox
authors.forename: Sabine
authors.initials: S
authors.lastname: Vancaeneghem
authors.forename: Bruno M
authors.initials: BM
authors.lastname: Goddeeris
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000378:metabolism*; D001422:Bacterial Adhesion; D015374:Biosensing Techniques; D020895:Enterocytes / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D036781:Fimbriae Proteins / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000379:methods*; D013552:Swine
chemical_list: D000942:Antigens, Bacterial; D029968:Escherichia coli Proteins; C026888:K88 antigen, E coli; D011956:Receptors, Cell Surface; D036781:Fimbriae Proteins