Regulation of Tilapia metallothionein gene expression by heavy metal ions.
Tilapia is a common fish species inhabiting inland waters and estuarine regions in Hong Kong and Southeast Asia, and useful for bio-monitoring of metal pollution. Metallothionein (MT) gene expression in fish tissues has been useful to sub-lethal risk assessment as biomarker of exposure to metal ions in fishes inhabiting metal contaminated area. To investigate metal inductions of Tilapia MT gene expression in vivo, Tilapias were injected with different concentrations of heavy metals and tissues were then removed for quantitative PCR assay using mimic PCR methods. All of the metal ions tested (Cu(2+), Cd(2+), Hg(2+), Ni(2+), Pb(2+) and Zn(2+)) were able to induce hepatic MT mRNA levels. Renal MT mRNA levels of Cd(2+) and Zn(2+) treated fish was not induced with significant fold induction, however MT mRNA levels in gills were sensitive to the administrations of these metal ions. These data indicated that Tilapia MT mRNA levels in gills and liver are sensitive biomarker of exposure to various metal ions.
Cheung
Andrew P L
AP
Department of Biochemistry, and Environmental Science Programme, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR, P.R. China.
Lam
Timothy H-J
TH
Chan
King Ming
KM
D000818:Animals; D015415:Biomarkers; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation; D005880:Gills / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008668:Metallothionein / Q000096:biosynthesis / Q000235:genetics*; D019216:Metals, Heavy / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism*; D017210:Tilapia / Q000235:genetics / Q000378:metabolism*
D015415:Biomarkers; D017931:DNA Primers; D018076:DNA, Complementary; D019216:Metals, Heavy; D012333:RNA, Messenger; D008668:Metallothionein