title: Microevolution between paired antral and paired antrum and corpus Helicobacter pylori isolates recovered from individual patients.
abstract: Sequence variations located at the signal sequence and mid-region within the vacA gene, the 3'-end of the cagA gene, the indel motifs at the 3'-end of the cag pathogenicity island and the regions upstream of the vacA and ribA genes were determined by PCR in 19 paired antral or antrum and corpus Helicobacter pylori isolates obtained at the same endoscopic session, and three antral pairs taken sequentially. Random amplification of polymorphic DNA (RAPD)-PCR and fluorescent amplified fragment length polymorphism (FAFLP)-PCR fingerprinting were applied to these paired clinical isolates. The FAFLP-PCR profiles generated were phylogenetically analysed. For the 22 paired isolates there were no differences within pairs at five of the genetic loci studied. However, six pairs of isolates (27%), of which four were antrum and corpus pairs, showed differences in the numbers of repeats located at the 3'-end of the cagA gene. RAPD-PCR fingerprinting showed that 16 (73%) pairs, nine of which were antrum and corpus pairs, possessed identical profiles, while six (27%) displayed distinctly different profiles, indicating mixed infections. Three of the six pairs showing differences at the 3'-end of the cagA gene yielded identical RAPD-PCR fingerprints. FAFLP-PCR fingerprinting and phylogenetic analysis revealed that all 16 pairs that displayed identical RAPD-PCR profiles had highly similar, but not identical, fingerprints, demonstrating that these pairs were ancestrally related but had undergone minor genomic alterations. Two antrum and corpus pairs of isolates, within the latter group, were isolates obtained from two siblings from the same family. This analysis demonstrated that each sibling was colonized by ancestrally related strains that exhibited differences in vacA genotype characteristics.
authors.affiliation: Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, University of Dublin, Dublin 2, Ireland 2Centre for DNA Fingerprinting and Diagnostics (CDFD), Nacharam, Hyderabad, 50 00076 India 3Owaisi Hospital and Research Centre, Deccan College of Medical Sciences, Kanchanbagh, Santoshnagar, Hyderabad, India 4Department of Gastroenterology, The Adelaide and Meath Hospital, Tallaght, Dublin 24, Ireland.
authors.forename: Ian M
authors.initials: IM
authors.lastname: Carroll
authors.affiliation: Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, University of Dublin, Dublin 2, Ireland 2Centre for DNA Fingerprinting and Diagnostics (CDFD), Nacharam, Hyderabad, 50 00076 India 3Owaisi Hospital and Research Centre, Deccan College of Medical Sciences, Kanchanbagh, Santoshnagar, Hyderabad, India 4Department of Gastroenterology, The Adelaide and Meath Hospital, Tallaght, Dublin 24, Ireland.
authors.forename: Niyaz
authors.initials: N
authors.lastname: Ahmed
authors.affiliation: Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, University of Dublin, Dublin 2, Ireland 2Centre for DNA Fingerprinting and Diagnostics (CDFD), Nacharam, Hyderabad, 50 00076 India 3Owaisi Hospital and Research Centre, Deccan College of Medical Sciences, Kanchanbagh, Santoshnagar, Hyderabad, India 4Department of Gastroenterology, The Adelaide and Meath Hospital, Tallaght, Dublin 24, Ireland.
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Beesley
authors.affiliation: Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, University of Dublin, Dublin 2, Ireland 2Centre for DNA Fingerprinting and Diagnostics (CDFD), Nacharam, Hyderabad, 50 00076 India 3Owaisi Hospital and Research Centre, Deccan College of Medical Sciences, Kanchanbagh, Santoshnagar, Hyderabad, India 4Department of Gastroenterology, The Adelaide and Meath Hospital, Tallaght, Dublin 24, Ireland.
authors.forename: Aleem A
authors.initials: AA
authors.lastname: Khan
authors.affiliation: Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, University of Dublin, Dublin 2, Ireland 2Centre for DNA Fingerprinting and Diagnostics (CDFD), Nacharam, Hyderabad, 50 00076 India 3Owaisi Hospital and Research Centre, Deccan College of Medical Sciences, Kanchanbagh, Santoshnagar, Hyderabad, India 4Department of Gastroenterology, The Adelaide and Meath Hospital, Tallaght, Dublin 24, Ireland.
authors.forename: Sheikh
authors.initials: S
authors.lastname: Ghousunnissa
authors.affiliation: Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, University of Dublin, Dublin 2, Ireland 2Centre for DNA Fingerprinting and Diagnostics (CDFD), Nacharam, Hyderabad, 50 00076 India 3Owaisi Hospital and Research Centre, Deccan College of Medical Sciences, Kanchanbagh, Santoshnagar, Hyderabad, India 4Department of Gastroenterology, The Adelaide and Meath Hospital, Tallaght, Dublin 24, Ireland.
authors.forename: Colm A Ó
authors.initials: CAÓ
authors.lastname: Moráin
authors.affiliation: Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, University of Dublin, Dublin 2, Ireland 2Centre for DNA Fingerprinting and Diagnostics (CDFD), Nacharam, Hyderabad, 50 00076 India 3Owaisi Hospital and Research Centre, Deccan College of Medical Sciences, Kanchanbagh, Santoshnagar, Hyderabad, India 4Department of Gastroenterology, The Adelaide and Meath Hospital, Tallaght, Dublin 24, Ireland.
authors.forename: C M
authors.initials: CM
authors.lastname: Habibullah
authors.affiliation: Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, University of Dublin, Dublin 2, Ireland 2Centre for DNA Fingerprinting and Diagnostics (CDFD), Nacharam, Hyderabad, 50 00076 India 3Owaisi Hospital and Research Centre, Deccan College of Medical Sciences, Kanchanbagh, Santoshnagar, Hyderabad, India 4Department of Gastroenterology, The Adelaide and Meath Hospital, Tallaght, Dublin 24, Ireland.
authors.forename: Cyril J
authors.initials: CJ
authors.lastname: Smyth
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D004381:Duodenal Ulcer / Q000382:microbiology; D019143:Evolution, Molecular; D006136:GTP Cyclohydrolase / Q000235:genetics; D005798:Genes, Bacterial; D005838:Genotype; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D010802:Phylogeny; D011110:Polymorphism, Genetic / Q000235:genetics; D011706:Pyloric Antrum / Q000382:microbiology; D019105:Random Amplified Polymorphic DNA Technique; D013270:Stomach / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori; D006136:GTP Cyclohydrolase; C111303:RibBA protein, Helicobacter pylori