title: Zymosan-induced luminol-dependent chemiluminescence response of circulating and extravasated leukocytes in experimental sepsis.
abstract: This study examines a concurrent profiling of circulating and extravasated polymorphonuclear leukocytes (PMNs) in a rat model of experimental sepsis. Fecal peritonitis was induced in Wistar male rats by intraperitoneal instillation of a fecal suspension in saline (1:1 w/v). Blood and peritoneal fluid were collected 8 h following fecal inoculation for the evaluation of inflammatory response of PMNs using zymosan-induced luminol-dependent chemiluminescence. Fifty microliters of pre-diluted blood or peritoneal fluid samples were mixed with 150 microl of reaction mixture (4 x 10(-4) M luminol+50 microg opsonized zymosan+0.1% gelatin in Hank's balanced salt solution) and the chemiluminescence signal was measured in a luminometer at 37 degrees C. Fecal peritonitis caused a significant leukocytopenia (3540+/-297 mm(-3) versus control value of 7525+/-711 mm(-3), p < 0.001) accompanied by massive infiltration of PMNs in the peritoneal cavity (34700+/-4006 versus 7325+/-425 mm(-3), p < 0.001). The phagocytic activity of circulating blood PMNs was down-regulated whereas a significant up-regulation was observed in the activity of PMNs from peritoneal fluid. In conclusion, this study clearly demonstrates sepsis-induced alterations in both blood and peritoneal fluid PMNs and their quantitative assessment may be helpful in disease evaluation and designing effective therapies.
authors.affiliation: Research Center, Riyadh Al Kharj Hospital Program, Saudi Arabia. khan_haseeb@yahoo.com
authors.forename: Haseeb Ahmad
authors.initials: HA
authors.lastname: Khan
mesh_terms: D000818:Animals; D001202:Ascitic Fluid / Q000737:chemistry; D005243:Feces / Q000382:microbiology; D049449:Luminescence; D008165:Luminol / Q000378:metabolism*; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D010587:Phagocytosis; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000139:chemically induced / Q000276:immunology*; D015054:Zymosan / Q000378:metabolism*
chemical_list: D008165:Luminol; D015054:Zymosan