[The polymorphism distributions of MICA and MICB microsatellite in Guangdong Han population].
OBJECTIVE
To investigate the genetic polymorphism of microsatellite in the exon 5 of MICA gene and the intron 1 of MICB gene in Guangdong Han population.


METHODS
One hundred and six samples of Guangdong Han population were genotyped by polymerase chain reaction and fluorescent technique (6-FAM). Gene frequency, power of discrimination, expected heterozygosity, polymorphism information content and probability of paternity exclusion were calculated.


RESULTS
The genotype distributions of MICA and MICB microsatellite met Hardy-Weinberg equilibrium. MICA A5 was the most common allele (0.2877), whereas A4 was the least popular one (0.1321). The genotype distribution frequencies of A5-5.1 (14.15%) and A5-5 (10.38%) are high. MICB CA14 was the most common allele (0.3255), and CA19,28 was the least popular one (0.0047). CA27 was not observed. The genotype distribution frequency of CA14-CA14(14.15%) is high.


CONCLUSIONS
The microsatellite of the exon 5 of MICA gene and the intron 1 of MICB gene could be used as the genetic markers of Chinese population in the studies of anthropology, linkage analysis of genetic disease genes, individual identification and paternity test in forensic medicine.
Feng
Ming-liang
ML
Shanghai Blood Center, Shanghai, 200051 PR China. fengml@sh163.net
Zhang
Jing-yi
JY
Xie
Jun-hua
JH
Ji
Yun
Y
Lu
Qiong
Q
Chen
Liang
L
Yang
Jian-hao
JH
Guo
Xiao-jun
XJ
D002681:China / Q000208:ethnology; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic
D015395:Histocompatibility Antigens Class I; C099187:MHC class I-related chain A; C098080:MICB antigen