title: The yeast genes, ARL1 and CCZ1, interact to control membrane traffic and ion homeostasis.
abstract: The yeast ARL1 gene, encoding a guanine-nucleotide binding protein of the Arf-like family, exhibits a synthetic genetic interaction with CCZ1. An arl1 Delta ccz1 Delta double mutant was viable but grew slowly, was more sensitive to caffeine, Ca(2+), Zn(2+), and hygromycin B than either single mutant, and had a more severe vacuolar protein sorting phenotype. Overexpression of ARL1 did not suppress ccz1 Delta mutant phenotypes, nor did overexpression of CCZ1 suppress arl1 Delta mutant phenotypes. We conclude that ARL1 and CCZ1 independently contribute to both ion homeostasis and protein sorting.
authors.affiliation: Department of Biology, Georgetown University, Washington, DC 20057, USA.
authors.forename: Sherie L
authors.initials: SL
authors.lastname: Love
authors.forename: Cara Marie A
authors.initials: CM
authors.lastname: Manlandro
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Testa
authors.forename: Anne E
authors.initials: AE
authors.lastname: Thomas
authors.forename: Kari-Elise
authors.initials: KE
authors.lastname: Tryggestad
authors.forename: Anne G
authors.initials: AG
authors.lastname: Rosenwald
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002110:Caffeine / Q000494:pharmacology; D043402:Cathepsin A / Q000378:metabolism; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics* / Q000378:metabolism*; D006706:Homeostasis; D006921:Hygromycin B / Q000494:pharmacology; D007477:Ions / Q000378:metabolism*; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D010641:Phenotype; D021381:Protein Transport / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; C441960:Ccz1 protein, S cerevisiae; D020662:Guanine Nucleotide Exchange Factors; D007477:Ions; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins; D002110:Caffeine; D006921:Hygromycin B; D043402:Cathepsin A; C487039:ARL1 protein, S cerevisiae; D020559:Monomeric GTP-Binding Proteins