title: Malaria vector anopheles (Nyssorhynchus) nuneztovari comprises one genetic species in colombia based on homogeneity of nuclear ITS2 rDNA.
abstract: Previous studies indicated that two distinct chromosomal forms of Anopheles nuneztovari Gabaldón, cytotypes B and C, occurred on the west and east of the Latin American Andes Mountains, respectively. To determine the taxonomic status ofAnopheles (Nyssorhynchus) nuneztovari in Colombia, link-reared specimens were collected from four sites: in the departments of Chocó (La Pacurita) and Valle (Sitronella) in the west, and Norte de Santander (Guaramito andl Tibú) in the east. Nuclear ITS2 sequences were generated for 46 individuals. Only two specimens (4.4%) showed divergent haplotypes, varying from the consensus by a single-base polymorphism (0.18%). These results suggest that populations of An. nuneztovari corresponding to cytotypes (B and C) are conspecific.
authors.affiliation: Programa de Estudio y Control de Enfermedades Tropicales, Facultad de Medicina, Universidad de Antioquia, Medellin, Colombia.
authors.forename: Diana M
authors.initials: DM
authors.lastname: Sierra
authors.forename: Ivan Dario
authors.initials: ID
authors.lastname: Velez
authors.forename: Yvonne-Marie
authors.initials: YM
authors.lastname: Linton
mesh_terms: D000818:Animals; D000852:Anopheles / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D002467:Cell Nucleus / Q000235:genetics*; D003105:Colombia; D016384:Consensus Sequence; D004275:DNA, Ribosomal / Q000235:genetics*; D005843:Geography; D006801:Humans; D008288:Malaria / Q000635:transmission*; D008969:Molecular Sequence Data; D011156:Population Density; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004275:DNA, Ribosomal