title: [Effect of curcumin on STAT5 signaling molecule in K562 cells].
abstract: OBJECTIVE
  To investigate the effect of curcumin on STAT 5 signaling molecule in K562 cells and its molecular mechanism of antileukemia.
  
  
  METHODS
  Cell proliferation was studied by tetrazolium dye assay. The expressions of STAT5 mRNA and protein were assayed by in situ hybridization, and Western blotting respectively.
  
  
  RESULTS
  Curcumin could inhibit K562 cell proliferation in a time- and dose-dependent manner. The percentage of STAT5-positive cells was 19% in curcunin group, significantly less than 31% of that in K562 cell group (P < 0.01). The A value of the expression level of STAT5 protein in curcumin group was 15 266 +/- 769, significantly less than 25 781 +/- 1240 of that in K562 cell group (P < 0.01).
  
  
  CONCLUSIONS
  The expressions of STAT5 mRNA and protein in K562 cells were inhibited by curcumin and curcumin could inhibit K562 cell proliferation.
authors.affiliation: Department of Hematology, Affiliated Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Jun-Xia
authors.initials: JX
authors.lastname: Gu
authors.forename: Jing
authors.initials: J
authors.lastname: He
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology*; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D050799:STAT5 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D050799:STAT5 Transcription Factor; D003474:Curcumin