title: Purification and functional assessment of C3a, C4a and C5a of the common carp (Cyprinus carpio) complement.
abstract: Promotion of inflammatory response is an important role of the complement system, but this kind of function is poorly documented for the lower vertebrates. Here we report chemotactic activity of purified anaphylactic fragments derived from the complement components C3, C4 and C5 of the common carp. The purified anaphylatoxins are two C5a-desArg peptides derived from the C5-I isotype, an intact form and a desArg form of C4a from C4-2 isotype, and an intact form and a desArg form of C3a from C3-H1 isoform. These were identified by N-terminal sequencing, mass spectrometry, and peptide mass fingerprinting. In the chemotaxis assay using carp kidney neutrophils, the two C5a-desArg fragments, which are probably allotypic variants, showed a potent chemotactic activity at 0.5-1 nM, whereas C3a or C4a showed no significant activity. The results suggest that C3a, C4a and C5a of bony fish have functionally diverged to the state similar to their mammalian homologs.
authors.affiliation: Laboratory of Marine Biochemistry, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kato
authors.forename: Miki
authors.initials: M
authors.lastname: Nakao
authors.forename: Motoyuki
authors.initials: M
authors.lastname: Shimizu
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Wariishi
authors.forename: Tomoki
authors.initials: T
authors.lastname: Yano
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002347:Carps / Q000276:immunology*; D002634:Chemotaxis, Leukocyte; D015926:Complement C3a / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D015934:Complement C4a / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D015936:Complement C5a / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000166:cytology; D010449:Peptide Mapping; D020033:Protein Isoforms / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020033:Protein Isoforms; D015926:Complement C3a; D015934:Complement C4a; D015936:Complement C5a