title: [Urotensin II induces hypertrophy of in vitro cultured neonatal rat cardiac myocytes].
abstract: OBJECTIVE
  To examine whether urotensinII (UII) induces hypertrophy of neonatal rat cardiomyocytes cultured in vitro.
  
  
  METHODS
  The primary cardiac myocytes cultured for 40 h followed by further culture in serum-free media for another 24 h were subjected to exposure to UII of varied concentrations for 24 h, after which the changes in the size of the cells were analyzed by flow cytometry with (3)H-leucine incorporation also measured.
  
  
  RESULTS
  At the concentration of 1x10(-7) mol/L, UIIcould increase the size of the cultured cardiac myocardial cells (P=0.021) and 3H-Leucine incorporation (P=0.015).
  
  
  CONCLUSIONS
  UII may induce hypertrophy of neonatal rat cardiac myocytes cultured in vitro.
authors.affiliation: Department of Cardiology, Guangzhou Red-Cross Hospital and the Fourth Hospital of Jinan University, Guangzhou 510220, China. hhzuo2002@sohu.com
authors.forename: Hui-hua
authors.initials: HH
authors.lastname: Zuo
authors.forename: Jie-min
authors.initials: JM
authors.lastname: Hong
authors.forename: Heng-shan
authors.initials: HS
authors.lastname: Guo
authors.forename: Jian-rui
authors.initials: JR
authors.lastname: Wei
authors.forename: Yan-hua
authors.initials: YH
authors.lastname: He
authors.forename: Zhi-liang
authors.initials: ZL
authors.lastname: Li
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D006332:Cardiomegaly / Q000139:chemically induced*; D002478:Cells, Cultured; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D043562:Receptors, G-Protein-Coupled / Q000032:analysis; D014579:Urotensins / Q000633:toxicity*
chemical_list: D043562:Receptors, G-Protein-Coupled; D014579:Urotensins; C473142:Uts2r protein, rat; C029677:urotensin II