Discovery of the chemical function of glycosidases: design, synthesis, and evaluation of mass-differentiated carbohydrate libraries.
[reaction: see text] Discovery of the catalytic chemical function of the many putative glycosidases coded in genomes currently relies on individual testing of possible substrates, usually as their p-nitrophenol conjugate. Herein, we present an alternative chemical proteomics approach using a synthetic mass-differentiated heat-stable substrate library with mass spectrometry readout. Library components do not serve as reaction inhibitors and both primary and secondary enzyme substrates can be delineated.
Yu
Yang
Y
Department of Chemistry and the Plant Sciences Institute, Gilman Hall, Iowa State University, Ames, Iowa 50011-3111, USA.
Ko
Kwang-Seuk
KS
Zea
Corbin J
CJ
Pohl
Nicola L
NL
D002241:Carbohydrates / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry; D002384:Catalysis; D020650:Combinatorial Chemistry Techniques; D005069:Evaluation Studies as Topic; D006026:Glycoside Hydrolases / Q000032:analysis / Q000737:chemistry; D020027:Pyrococcus furiosus / Q000201:enzymology; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D002241:Carbohydrates; D006026:Glycoside Hydrolases