Caenorhabditis elegans development requires mitochondrial function in the nervous system.
The mitochondrial respiratory chain (MRC) supplies the majority of the energy requirements of most eucaryotic cells. A null mutation in the Caenorhabditis elegans nuo-1 gene encoding a subunit of complex I (NADH-ubiquinone oxidoreductase) is lethal, leading to a developmental arrest at the third larval stage. To identify the tissues that regulate development in response to mitochondrial dysfunction, we restored nuo-1 expression with tissue-specific promoters. Only expression of nuo-1 ubiquitously or in the nervous system supported development to the adult stage. Pharyngeal expression of nuo-1 allowed development to proceed to the fourth larval stage. Expression of nuo-1 in the body muscles or in the germline had no effect. Furthermore, only ubiquitous or nervous system expression of nuo-1 allowed exit from the dauer state. Our results indicate that MRC function in the nervous system is needed to send and receive signals that control larval development and exit from dauer.
Ndegwa
Sarah
S
Department of Biochemistry, Membrane Protein Research Group of the Canadian Institutes of Health Research, University of Alberta, Edmonton, Alta., Canada T6G 2H7.
Lemire
Bernard D
BD
D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000033:anatomy & histology* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D004579:Electron Transport / Q000502:physiology; D042967:Electron Transport Complex I / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D009154:Mutation; D009420:Nervous System / Q000033:anatomy & histology / Q000378:metabolism*; D010641:Phenotype; D011401:Promoter Regions, Genetic; D019076:Transgenes
D029742:Caenorhabditis elegans Proteins; D042967:Electron Transport Complex I; C488200:NUO-1 protein, C elegans