title: Gene expression profiling of 1p35-36 genes in neuroblastoma.
abstract: Deletion of the chromosome 1p36 region is a frequent abnormality in neuroblastoma. To gain further insights into the role of this alteration in oncogenesis, we have constructed a specific cDNA microarray representing most known genes and ESTs from the 1p35-36 region and analysed the expression profiles of 15 neuroblastoma cell lines and 28 neuroblastoma tumours. Hierarchical clustering using expression levels of 320 cDNAs from 1p35-36 separated localized or 4S cases without 1p deletion from advanced stages and cell lines. Supervised learning classification enabled to predict reliably the status of chromosome 1p according to its expression profile. Around 15% of the genes or ESTs presented a significantly decreased expression in samples with 1p deletion as compared to 1p-normal samples suggesting that 1p deletion results in a gene dosage effect on a subset of genes critical for the development of 1p-deleted neuroblastoma. Several genes presumed to have functions in neural differentiation (CDC42, VAMP3, CLSTN1), signal transduction in neural cells (GNB1) and cell cycle regulation (STMN1, RPA2, RBAF600, FBXO6, MAD2L2) exhibited a decreased expression in samples presenting 1p deletion. The identification of such genes provides baseline information for further studies to elucidate how these genes could individually or collectively play a critical role in neuroblastoma tumorigenesis.
authors.affiliation: INSERM U509, Laboratoire de Pathologie Moléculaire des Cancers, Institut Curie--Section de Recherche, Pavillon Trouillet-Rossignol 26, rue d'Ulm, 75248 Paris Cedex 05, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Janoueix-Lerosey
authors.forename: Eugene
authors.initials: E
authors.lastname: Novikov
authors.forename: Marta
authors.initials: M
authors.lastname: Monteiro
authors.forename: Nadège
authors.initials: N
authors.lastname: Gruel
authors.forename: Gudrun
authors.initials: G
authors.lastname: Schleiermacher
authors.forename: Béatrice
authors.initials: B
authors.lastname: Loriod
authors.forename: Catherine
authors.initials: C
authors.lastname: Nguyen
authors.forename: Olivier
authors.initials: O
authors.lastname: Delattre
mesh_terms: D045744:Cell Line, Tumor; D002878:Chromosomes, Human, Pair 1; D020869:Gene Expression Profiling; D006801:Humans; D019656:Loss of Heterozygosity; D009154:Mutation; D009447:Neuroblastoma / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic